{ "genome": { "genus": "Mycoplasmopsis", "species": "fermentans", "strain": "PG18", "taxon": "Mycoplasmopsis fermentans PG18", "complete": false, "gram": "?", "translation_table": 11 }, "stats": { "size": 1004014, "gc": 0.26870804649092517, "n_ratio": 0.002003956120133783, "n50": 1004014, "n90": 1004014, "coding_ratio": 0.6512332310713951 }, "features": [ { "type": "gap", "sequence": "contig_1", "start": 184, "stop": 184, "strand": ".", "length": 1, "id": "OAIKOHHJFB_1786" }, { "type": "gap", "sequence": "contig_1", "start": 252, "stop": 252, "strand": ".", "length": 1, "id": "OAIKOHHJFB_1787" }, { "type": "gap", "sequence": "contig_1", "start": 291, "stop": 291, "strand": ".", "length": 1, "id": "OAIKOHHJFB_1788" }, { "type": "gap", "sequence": "contig_1", "start": 419, "stop": 419, "strand": ".", "length": 1, "id": "OAIKOHHJFB_1789" }, { "type": "gap", "sequence": "contig_1", "start": 478, "stop": 478, "strand": ".", "length": 1, "id": "OAIKOHHJFB_1790" }, { "type": "gap", "sequence": "contig_1", "start": 497, "stop": 497, "strand": ".", "length": 1, "id": "OAIKOHHJFB_1791" }, { "type": "cds", "sequence": "contig_1", "start": 832, "stop": 984, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013527148.1", "SO:0001217", "UniParc:UPI0001F59906", "UniRef:UniRef100_A0AB32XDD3", "UniRef:UniRef50_A0A168RBX0", "UniRef:UniRef90_A0AB32XDD3" ], "nt": "ATGAGAATACAAAATTTTAAGCGTGATAAATTTAAATCTAAATTTGTATTATTAAACTTTTGCGAAGATAAAAATCCAGATCTTCCATTTTTTGTTATTGAGTATTCTCAAAACGTGTTTGATTTAGCCTCCCTAAGAAGTCAATCGATTTAA", "aa": "MRIQNFKRDKFKSKFVLLNFCEDKNPDLPFFVIEYSQNVFDLASLRSQSI", "aa_hexdigest": "ca5310ae2eb824651c99281c974007b1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59906", "ncbi_nrp_id": "WP_013527148.1", "uniref100_id": "UniRef100_A0AB32XDD3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59906", "RefSeq:WP_013527148.1", "UniRef:UniRef100_A0AB32XDD3" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XDD3", "uniref90_id": "UniRef90_A0AB32XDD3" }, "psc": { "uniref90_id": "UniRef90_A0AB32XDD3", "uniref50_id": "UniRef50_A0A168RBX0" }, "pscc": { "uniref50_id": "UniRef50_A0A168RBX0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A168RBX0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6017.886799999999, "isoelectric_point": 8.977689933776855 }, "id": "OAIKOHHJFB_46", "locus": "GCA000209735_00001" }, { "type": "cds", "sequence": "contig_1", "start": 1105, "stop": 1500, "strand": "+", "frame": 1, "gene": null, "product": "Multiple sugar ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEI0", "UniRef:UniRef90_A0A3P8MEI0" ], "nt": "ATGATTAGGAAAAATAATACACCTTATATTATTGAATTAAAAGAAGTAGTTAAAGAATTCGATGATAAGGTTGTTTTGGACAATGTTGATTTAAAAATCAATAAAGGTGAATTTGTTACTTTGCTAGGACCTTCAGGATCTGGCAAAACTACAATCCTTAGACTTATTGCTGGTTTTGAAAGAGCAACACGTGGTGAAATTAAATTTCATGGTGTAGATATTAAAGACTTGCCGCCTCACAAAAGAGACCTTTCAACTATTTTTCAAGATTATGCATTATTTCCTCACTTAGATGTTGAAGGAAACATTAAATATGGTTTAGCTCTTAAAAGAAAACCAAAAGAAGTTATTAATGCTAAACATGAAAAATTATTAGTTCAAAAACAAAAACAATGA", "aa": "MIRKNNTPYIIELKEVVKEFDDKVVLDNVDLKINKGEFVTLLGPSGSGKTTILRLIAGFERATRGEIKFHGVDIKDLPPHKRDLSTIFQDYALFPHLDVEGNIKYGLALKRKPKEVINAKHEKLLVQKQKQ", "aa_hexdigest": "132d9bd15df8f049e074421499d0a8cf", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 1105, "stop": 1698, "observations": { "mutation": [ 1498, 1612, 1645 ], "stop": [ 1498, 1612, 1645 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8MEI0", "query_cov": 0.9923664122137404, "subject_cov": 0.6372549019607843, "identity": 0.9540000000000001, "score": 246.0, "evalue": 1.55e-81, "gene_start": 1, "gene_end": 130, "reference_start": 1, "reference_end": 130, "reference_sequence": "MIKKNNSPYIIELKEVVKEFDDKVVLENVDLKINKGEFVTLLGPSGSGKTTILRLIAGFERATRGEIKFHGVDIKDLPPHKRDLSTIFQDYALFPHLNVEGNIKYGLALKRMPKEVINAKHEKLLAQKQKKWEERAKAEMKKLDVLQTKYEREMKTLKKNTFAYRRRQKWLDKSDFNYSYWENYVDQQTENFEKRYLPSLTNQK" }, "paralog": false, "identity": 0.7614213197969543, "score": 285.0, "evalue": 2.96e-99, "description": "Internal stop codon at 1498, 1612, 1645. Nonsense mutation around 1498, 1612, 1645.", "psc": { "uniref90_id": "UniRef90_A0A3P8MEI0", "product": "Multiple sugar ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A3P8MEI0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8MEI0", "UniRef:UniRef50_A0A3P8MEI0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MEI0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEI0" ], "product": "Multiple sugar ABC transporter ATP-binding protein" } }, "id": "OAIKOHHJFB_47", "locus": "GCA000209735_00002" }, { "type": "cds", "sequence": "contig_1", "start": 1522, "stop": 1614, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATTAGATGCTCTACAAGAAAAATATGAAAGAGAAATGGAAACATTAAAACCAAATACTTTTGCATATCGTCGTAGACAAAAATGA", "aa": "MKKLDALQEKYEREMETLKPNTFAYRRRQK", "aa_hexdigest": "84a39694df3e377086249004b9412e74", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3802.3878000000013, "isoelectric_point": 9.867998313903808 }, "id": "OAIKOHHJFB_48", "locus": "GCA000209735_00003" }, { "type": "cds", "sequence": "contig_1", "start": 1720, "stop": 2079, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021D517A5" ], "nt": "ATGGACCAAGAAGTTAAAGAAATTGTAGAATTAGTCGGACTTCAAGGTAATGAAAAAAGATCTATTAATGAACTTTCGGGTGGTATGAAACAACGTGTTGCTTTAGCCAGATCTCTTGTTATTGAACCTGCTATTCTTTTATTAGATGAACCTCTTAGTGCTCTTGATGCCAAAATTAGACAAAAAATGCAAATATTATTAAGAAGCTTACAACAAAAATTAGGTCTTACATTTATTTTTGTTACACATGATCAAGATGAAGCTCTTGAACTTTCAGATAGAATTGCAATTATGCGTGGTGGAAAAATTGAACAATATGACACGCCAAAAAATATTTATGACTATCCAGTTAATAAATGA", "aa": "MDQEVKEIVELVGLQGNEKRSINELSGGMKQRVALARSLVIEPAILLLDEPLSALDAKIRQKMQILLRSLQQKLGLTFIFVTHDQDEALELSDRIAIMRGGKIEQYDTPKNIYDYPVNK", "aa_hexdigest": "65238b594da9ee4225894c7f760cd10d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021D517A5", "query_cov": 0.9663865546218487, "subject_cov": 0.8273381294964028, "identity": 0.53, "score": 123.0, "evalue": 5.73e-34, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI0021D517A5" }, "pscc": { "uniref50_id": "UniRef50_UPI0021D517A5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021D517A5" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "OAIKOHHJFB_49", "locus": "GCA000209735_00004" }, { "type": "cds", "sequence": "contig_1", "start": 2242, "stop": 2406, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTATGATGAAAAGAAAAAAGACAAGCTTGTTGGTGAAATTATTAACATGGCTTACCGTGGAAGTTATTACTACTTAAAAATTCAACTTGACAATGATGAAATCATTTATGTTGAAACAGCTAAAAAATTCGAAATAGGTGAAAAAGTAAACTTAAGCTGA", "aa": "MAYDEKKKDKLVGEIINMAYRGSYYYLKIQLDNDEIIYVETAKKFEIGEKVNLS", "aa_hexdigest": "eb4577405dac820bbf002ec5575ec7dc", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6382.2701, "isoelectric_point": 5.277580070495604 }, "id": "OAIKOHHJFB_50", "locus": "GCA000209735_00005" }, { "type": "cds", "sequence": "contig_1", "start": 2462, "stop": 2638, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTCAAAAATTAAAAACAAACTTGGTTTAAATAAAAGATTACTACTTTTATTACCTTATTTATTTATTGCTATCTTTTTAATAATTTTGCCAATTTTATTAATCGTAATTAATGCTTTAAAACCACAAGATGGCTTTAATGCATCAGCTTTATTAGCTGAAAAAGGAATTTGA", "aa": "MNSKIKNKLGLNKRLLLLLPYLFIAIFLIILPILLIVINALKPQDGFNASALLAEKGI", "aa_hexdigest": "29f58f503bfeb1c9cc758624beca915a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6428.9259999999995, "isoelectric_point": 10.121810340881346 }, "id": "OAIKOHHJFB_51", "locus": "GCA000209735_00006" }, { "type": "cds", "sequence": "contig_1", "start": 2870, "stop": 3280, "strand": "+", "frame": 2, "gene": null, "product": "ABC transmembrane type-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDP5", "UniRef:UniRef90_C4XDP5" ], "nt": "ATGGTTTTTGGTTTAACTTATTTAAACTTGCCATACATGATTATGCCACTTTACACAGTCTTTAAAGACATGCCTAAAAACATTCTTGAAGCTAGTGCAGACTTAGGTTACAACAAATTTCAAACATTATTTAAAGTAGTTGTTCCATATAGTTTCAAAGCTATTTTAAGTGGTTTCAGTATGATCTTCTTATCATCAGCAACCACCTTTGTTATTAGTGCAAAATTACTTCCAAATGGTACTCAATTACAAACTATTGGATCTGTAATTAATGATTATTCAAGCCCTTCAAACAAATATGAATTAGCTTTAGGAAGCACACTTGTTTTAGTTGTATCAGCCATATTTATAGGTTGTTACGGATTAATCAATTTTGTTCCTAAAATTATAATTAGACTTAAAAAAAACTAA", "aa": "MVFGLTYLNLPYMIMPLYTVFKDMPKNILEASADLGYNKFQTLFKVVVPYSFKAILSGFSMIFLSSATTFVISAKLLPNGTQLQTIGSVINDYSSPSNKYELALGSTLVLVVSAIFIGCYGLINFVPKIIIRLKKN", "aa_hexdigest": "b68dbcb0c8256da2e8bc05779c125563", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 2570, "stop": 3302, "observations": { "insertion": [ 3266 ], "mutation": [ 2636, 2648, 2864 ], "stop": [ 2636, 2648, 2864 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDP5", "query_cov": 1.0, "subject_cov": 0.4874551971326165, "identity": 0.993, "score": 261.0, "evalue": 5.3e-86, "gene_start": 1, "gene_end": 136, "reference_start": 137, "reference_end": 272, "reference_sequence": "MNSKIKNKLGLNKRLLLLLPYLFIAIFLIILPILLIVINALKPQDGFNASALLAEKGIWLIIWRSLKIGLISAILCLIIAFPYAYFIATTKSKYLAIYALSLILSPMIIFTIAKIYAIRGFFLSIFEEDSLNAEWFMVFGLTYLNLPYMIMPLYTVFKDMPKNILEASADLGYNKFQTLFKVVVPYSFKAILSGFSMIFLSSATTFVISAKLLPNGTQLQTIGSVINDYSSPSNKYELALGSTLVLVVSAIFIGCYGLINFVPKIIIRLKKTKGGKKHD" }, "paralog": false, "identity": 0.9876543209876543, "score": 452.0, "evalue": 1.12e-163, "description": "Internal stop codon at 2636, 2648, 2864. Frameshift due to insertion around 3266. Nonsense mutation around 2636, 2648, 2864.", "psc": { "uniref90_id": "UniRef90_C4XDP5", "product": "ABC transmembrane type-1 domain-containing protein", "uniref50_id": "UniRef50_C4XDP5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDP5", "UniRef:UniRef50_C4XDP5" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDP5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDP5" ], "product": "ABC transmembrane type-1 domain-containing protein" } }, "id": "OAIKOHHJFB_52", "locus": "GCA000209735_00007" }, { "type": "cds", "sequence": "contig_1", "start": 3709, "stop": 3846, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAGAGGAATAGTAGGTCACACAATTATGGCTTTACCTTATGGTATTACTTTAATGTATCCAAGAAGTGAAAAATTCAATGCTTCATTATTTGAAGCGAGCCAAGATTTAGGTTATTCAAAAATTAGATCATGA", "aa": "MIRGIVGHTIMALPYGITLMYPRSEKFNASLFEASQDLGYSKIRS", "aa_hexdigest": "5a6f6131de25d47b54e7db228b0d492f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5063.871700000001, "isoelectric_point": 9.396283149719238 }, "id": "OAIKOHHJFB_53", "locus": "GCA000209735_00008" }, { "type": "cds", "sequence": "contig_1", "start": 3868, "stop": 3999, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTCCATCAATTGTTTTTGCTGGTTTAGTTTCCATTTTCTTATCATTTGATGACTTTATTATTTTACAAACAACTTCAAATGTAAGTACACTTGGAACCAAACTTTATGAAGGTCAATTTAAAGCTTGA", "aa": "MIPSIVFAGLVSIFLSFDDFIILQTTSNVSTLGTKLYEGQFKA", "aa_hexdigest": "583831a1e41804129e9a082136436fe3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4713.4439, "isoelectric_point": 4.556577491760254 }, "id": "OAIKOHHJFB_54", "locus": "GCA000209735_00009" }, { "type": "cds", "sequence": "contig_1", "start": 4115, "stop": 4849, "strand": "+", "frame": 2, "gene": "potD", "product": "PotD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8K8Q4", "UniRef:UniRef90_Q6GZ00" ], "nt": "ATGAACAACAACAAACCAAAGAACAACAAATTTAAAACACTTATTAAAACCAAAATTTTAACCAAAACTTTTGGTACAAGTGCTCTTGCAGTTGTTGCTGCAGCTGCTTTAGTTGGTGCTTTATCTTATAAATATAGTAACAAAAATAACTTCAAGCCTTCATTCTACAACTATCAATCTTATATTGATCCAAATACCAAAGCAAAAATAAATAAAAACTTCAACTTCAAAGAATTTGCTGAAATTCAAGATTTTACTAAAGCTATTTTAACTAATAAAGCAGCTGCCGGAATTGGCAACGATTCTCAAGCAGTTCAACTTATTAGAGAGGGTAAACTTCGCAAAATTGATTATGCTAAAATGTTTGGTGATCCTAAATTTAATGACCGCAATGAAGTTTTAAAACTTTTAACTGACACAGTAAAAAATCATCTTGAGTCATATAACAAATATTTAACTAAAGATAAATGTATTAATGCCGATGGTTCGCAAGCTACTGAGGATCGTCACTTAATTGACTACTTTGCTCCATACTTTTCACAAGATATGGTAGTAGCTTATAATCCTGCTAAAGTTTTAAATCTTAAAAAAGATGACCCTGAATATCAAAATAAAATCAATGCTCATCAACAAGCAATGCAAAATCACCTAATTAATGAAATAAAAAACAACAAAGGTGAATTTAGACTCATTGATATTCTTAAAACATTAAAAGCTAACAAATATGAACATTGA", "aa": "MNNNKPKNNKFKTLIKTKILTKTFGTSALAVVAAAALVGALSYKYSNKNNFKPSFYNYQSYIDPNTKAKINKNFNFKEFAEIQDFTKAILTNKAAAGIGNDSQAVQLIREGKLRKIDYAKMFGDPKFNDRNEVLKLLTDTVKNHLESYNKYLTKDKCINADGSQATEDRHLIDYFAPYFSQDMVVAYNPAKVLNLKKDDPEYQNKINAHQQAMQNHLINEIKNNKGEFRLIDILKTLKANKYEH", "aa_hexdigest": "e1bfe170e3e67bb5f4a7d5dea9992b56", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "potD" ], "truncated": "3-prime", "pseudogene": { "start": 4115, "stop": 5152, "observations": { "mutation": [ 4847, 4970 ], "stop": [ 4847, 4970 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q6GZ00", "query_cov": 1.0, "subject_cov": 0.4756335282651072, "identity": 1.0, "score": 476.0, "evalue": 8.75e-166, "gene_start": 1, "gene_end": 244, "reference_start": 1, "reference_end": 244, "reference_sequence": "MNNNKPKNNKFKTLIKTKILTKTFGTSALAVVAAAALVGALSYKYSNKNNFKPSFYNYQSYIDPNTKAKINKNFNFKEFAEIQDFTKAILTNKAAAGIGNDSQAVQLIREGKLRKIDYAKMFGDPKFNDRNEVLKLLTDTVKNHLESYNKYLTKDKCINADGSQATEDRHLIDYFAPYFSQDMVVAYNPAKVLNLKKDDPEYQNKINAHQQAMQNHLINEIKNNKGEFRLIDILKTLKANKYEHWEATNAVRDNMIYGSAYVYDVNKGFVDDHATGKGTSKDKPNWYKEHIDHFVELFNHGIGYSVRDTKHINFNGDGQFLLNNLINPDSKTCAGLIYNGDAVDAYFSEDNYATVPQSTIRFLRPKVNLLLVDGLVISENTSDYFTDLIYKTVHKSFLGGVEKDKWINGPKWNSTTEEWNNIEGYESFTTFDFVGYTPAWKSQYEFVKNNYFVDDKYPQYIASLYGINKEQTIFDVDNTTPKMKYEVKHFPIEPESNQPMAEIKVYYDTKLKS" }, "paralog": false, "identity": 0.9942028985507246, "score": 696.0, "evalue": 8.82e-255, "description": "Internal stop codon at 4847, 4970. Nonsense mutation around 4847, 4970.", "psc": { "uniref90_id": "UniRef90_Q6GZ00", "product": "PotD", "uniref50_id": "UniRef50_A0A3P8K8Q4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q6GZ00", "UniRef:UniRef50_A0A3P8K8Q4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8K8Q4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8K8Q4" ], "product": "Hypothetical lipoprotein (MG045 family)" } }, "id": "OAIKOHHJFB_55", "locus": "GCA000209735_00010" }, { "type": "cds", "sequence": "contig_1", "start": 4877, "stop": 4972, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTATGGTTCAGCTTATGTTTATGACGTTAATAAAGGCTTTGTTGATGATCACGCTACTGGAAAAGGCACATCAAAAGATAAGCCTAATTGA", "aa": "MIYGSAYVYDVNKGFVDDHATGKGTSKDKPN", "aa_hexdigest": "261fd1280e2c1c445ec61071177c4d8f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3378.677200000001, "isoelectric_point": 6.505472755432128 }, "id": "OAIKOHHJFB_56", "locus": "GCA000209735_00011" }, { "type": "cds", "sequence": "contig_1", "start": 5177, "stop": 5332, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTCAAAGTACTATTAGATTCTTAAGACCAAAAGTTAATTTATTATTAGTTGATGGGTTAGTTATCTCAGAAAATACAAGTGATTATTTTACTGATTTAATTTATAAAACAGTTCACAAGAGCTTTCTTGGTGGAGTTGAAAAAGATAAATGA", "aa": "MPQSTIRFLRPKVNLLLVDGLVISENTSDYFTDLIYKTVHKSFLGGVEKDK", "aa_hexdigest": "2300050d00982d2ac0e47781460d3841", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5827.7034, "isoelectric_point": 8.085769844055175 }, "id": "OAIKOHHJFB_57", "locus": "GCA000209735_00012" }, { "type": "cds", "sequence": "contig_1", "start": 5561, "stop": 5656, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATATGAAGTTAAACATTTTCCTATTGAACCAGAAAGTAAACAAACAATGGCTGAAATCAAAGTTTACTATGATACAAAACTAAAAAGTTAA", "aa": "MKYEVKHFPIEPESKQTMAEIKVYYDTKLKS", "aa_hexdigest": "1de529985a91ef3d671f0a9d10a6f1d5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3762.3519000000006, "isoelectric_point": 8.029940223693849 }, "id": "OAIKOHHJFB_58", "locus": "GCA000209735_00013" }, { "type": "cds", "sequence": "contig_1", "start": 5948, "stop": 6226, "strand": "+", "frame": 2, "gene": null, "product": "AAA domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDP8", "UniRef:UniRef50_UPI000FF29B85", "UniRef:UniRef90_C4XDP8", "UniRef:UniRef90_UPI000FF29B85" ], "nt": "ATGAATGAAATAAAAGAATTGTTTAATAACAAAAATGTTAAATCTTATGAAGTTGATGATTTAATTAAATTTATTTCTTTTGCAAAAAGCGGTTTTGAATTTGTGCCAAATGAAACTTTAATATTTTTTGATGAAATTCAAGAACAACCTAATTGCATTACAAGTTTAAAAAGTTTTGCCTTAGACAAAAGATTTGATGTTATTTGCTCAGGTTTTTTATTAGGAATTAATTTTAAAGAACACAATATTAGACACATTGGAGTCGGTTATATTTCTTAG", "aa": "MNEIKELFNNKNVKSYEVDDLIKFISFAKSGFEFVPNETLIFFDEIQEQPNCITSLKSFALDKRFDVICSGFLLGINFKEHNIRHIGVGYIS", "aa_hexdigest": "c7c822c944341a0033cd2393e79e7013", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000FF29B85", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.9890000000000001, "score": 186.0, "evalue": 4.78e-60, "valid": true, "uniref50_id": "UniRef50_UPI000FF29B85" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF29B85", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF29B85" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 5786, "stop": 6226, "observations": { "mutation": [ 5822 ], "stop": [ 5822 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDP8", "query_cov": 1.0, "subject_cov": 0.6301369863013698, "identity": 1.0, "score": 189.0, "evalue": 3.25e-60, "gene_start": 1, "gene_end": 92, "reference_start": 55, "reference_end": 146, "reference_sequence": "MLKRKIDAFLLDWKNDANKKPLIIKGARQIGKTTSILEFAKNNYKYVVYINFALMNEIKELFNNKNVKSYEVDDLIKFISFAKSGFEFVPNETLIFFDEIQEQPNCITSLKSFALDKRFDVICSGFLLGINFKEHNIRHIGVGYIS" }, "paralog": false, "identity": 0.9931506849315068, "score": 290.0, "evalue": 2.15e-102, "description": "Internal stop codon at 5822. Nonsense mutation around 5822.", "psc": { "uniref90_id": "UniRef90_C4XDP8", "product": "AAA domain-containing protein", "uniref50_id": "UniRef50_C4XDP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDP8", "UniRef:UniRef50_C4XDP8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDP8" ], "product": "AAA domain-containing protein" } }, "id": "OAIKOHHJFB_59", "locus": "GCA000209735_00014" }, { "type": "cds", "sequence": "contig_1", "start": 6711, "stop": 7565, "strand": "+", "frame": 3, "gene": null, "product": "SF4 helicase domain-containing protein", "db_xrefs": [ "EC:5.6.2.3", "KEGG:K02314", "RefSeq:WP_013526930.1", "SO:0001217", "UniParc:UPI00003B49A9", "UniRef:UniRef100_C4XDP9", "UniRef:UniRef50_C4XDP9", "UniRef:UniRef90_C4XDP9" ], "nt": "ATGCTACTCACTACAAAACAGTTACAGGAATTTTACAAAATTGAAATGGAAAATATTGCAAAAAATAAATCTCTATTAACTAACTTCAATATCATTGATAATTATGCAAATGGTTTAAAACCTAAACAACTTTATTTTTTAGCTGCAAGACCAGGCGTTGGAAAAACTTCTTTTGCTCTTAACATTATTTTTAATGCTTTGGAAAATTTGAAAGAAGATGAATGCATTGTTTTGTTTAGTCTTGAAATGGACTCAATTGAAATATATTCAAAACTTTTAGCTCTTGCCAATTCTTGTTCACTTAAAGAAGTTGAAACAAATTCTAAGAAAATAGATTTTGAAGATTTAAACAAAAAGAACCTTTTTATATTTGATAGATATAGAAACAATTTTAATAATGAAAAATTAAGTGAAGTGACTCCTGACATTATTTATAAAAGTTTGAATTCTATTGGTTTAAAGATCAAAGCTGTTTTTATTGATTATTTTCAATTGCTTGATAATCAAAAATATTCAAGTGAAAGAGAAAAGTTGTCTCAATGCTCTAAACAATTAAAAGAATTGTCAAAAATATTTAATTGTAATTTTTTTGTTTTATCTCAACTTTCAAGAGAGTATGAAAAGAAAACCAATAATGAACCATCTTTTTCTGATTTAAAAGGAACTGGTTCTATTGAACAAGACGCTGATTTAATTATGTTTCTTTATAATTCTTCAAATAATTTTAATGTTGGCACTTTAATCCCTAACAAGAAAACAATTTTGAACTTATCAATTGCAAAAAATAGAAATGGTGAATTGGTCAAAACAAATATTGATTTTATTCCGCATTTAGCAAAATTTATAGAAAGTTAA", "aa": "MLLTTKQLQEFYKIEMENIAKNKSLLTNFNIIDNYANGLKPKQLYFLAARPGVGKTSFALNIIFNALENLKEDECIVLFSLEMDSIEIYSKLLALANSCSLKEVETNSKKIDFEDLNKKNLFIFDRYRNNFNNEKLSEVTPDIIYKSLNSIGLKIKAVFIDYFQLLDNQKYSSEREKLSQCSKQLKELSKIFNCNFFVLSQLSREYEKKTNNEPSFSDLKGTGSIEQDADLIMFLYNSSNNFNVGTLIPNKKTILNLSIAKNRNGELVKTNIDFIPHLAKFIES", "aa_hexdigest": "4a93e6f8cdf2090e0fd7683161a28ac6", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00003B49A9", "ncbi_nrp_id": "WP_013526930.1", "uniref100_id": "UniRef100_C4XDP9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00003B49A9", "RefSeq:WP_013526930.1", "UniRef:UniRef100_C4XDP9" ] }, "ips": { "uniref100_id": "UniRef100_C4XDP9", "uniref90_id": "UniRef90_C4XDP9" }, "psc": { "uniref90_id": "UniRef90_C4XDP9", "product": "SF4 helicase domain-containing protein", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_C4XDP9", "kegg_orthology_id": "K02314" }, "pscc": { "uniref50_id": "UniRef50_C4XDP9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDP9" ], "product": "SF4 helicase domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_60", "locus": "GCA000209735_00015" }, { "type": "cds", "sequence": "contig_1", "start": 7728, "stop": 7889, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013526932.1", "SO:0001217", "UniParc:UPI00003B49BC", "UniRef:UniRef100_C4XDQ0", "UniRef:UniRef50_C4XDQ0", "UniRef:UniRef90_C4XDQ0" ], "nt": "ATGAATAAAGAAAAACTGATTAAATTTCTTATTGATTTAACAGAAAAAGAAAATACAAAATTAAAATCAGATGTTAAAATTCTAAAATCTAAACTTAAAAAAATAAAAAAAGAATATGAAGAACAAGAAAAAGCTTTTGATGACTTATTAAAAAAACTTTAG", "aa": "MNKEKLIKFLIDLTEKENTKLKSDVKILKSKLKKIKKEYEEQEKAFDDLLKKL", "aa_hexdigest": "b9b9b01faeae9033cd045880907e0c37", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI00003B49BC", "ncbi_nrp_id": "WP_013526932.1", "uniref100_id": "UniRef100_C4XDQ0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00003B49BC", "RefSeq:WP_013526932.1", "UniRef:UniRef100_C4XDQ0" ] }, "ips": { "uniref100_id": "UniRef100_C4XDQ0", "uniref90_id": "UniRef90_C4XDQ0" }, "psc": { "uniref90_id": "UniRef90_C4XDQ0", "uniref50_id": "UniRef50_C4XDQ0" }, "pscc": { "uniref50_id": "UniRef50_C4XDQ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDQ0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6395.590999999999, "isoelectric_point": 9.488601875305175 }, "id": "OAIKOHHJFB_61", "locus": "GCA000209735_00016" }, { "type": "cds", "sequence": "contig_1", "start": 7926, "stop": 8132, "strand": "+", "frame": 3, "gene": null, "product": "DNA cytosine methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021806FCC", "UniRef:UniRef90_UPI0021806FCC" ], "nt": "TTGTATGATGACGGCAATATGAGTTATTTTAAAGCATTACAAAACAATGAAAATTGTGTTATTTATTCTATAGGAATACAAGAACATAAAGATTTAAAGAATTATTATAATATTGACCTTTCATTAAATAATTTTAATTTAATTGACCAACTAAAACTAATTCCAAAACCTGACATTATAATTGCTTCTCCACCTTGTGAGAGTTGA", "aa": "MYDDGNMSYFKALQNNENCVIYSIGIQEHKDLKNYYNIDLSLNNFNLIDQLKLIPKPDIIIASPPCES", "aa_hexdigest": "3e37fdb238bb2c41f499446157961cd2", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 7893, "stop": 8277, "observations": { "insertion": [ 8226 ], "mutation": [ 7920, 8130, 8160, 8184 ], "stop": [ 7920, 8130, 8160, 8184 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0021806FCC", "query_cov": 1.0, "subject_cov": 0.5354330708661418, "identity": 0.941, "score": 136.0, "evalue": 4.9e-40, "gene_start": 1, "gene_end": 68, "reference_start": 12, "reference_end": 79, "reference_sequence": "MNENKKITIWALYDDGNMSYFKALQNNENCVIYSIGIQEHKDLKNYYNIDFSLNNVNLINQLKLIPKPDIIIASPPCESWSRADCSLAIWKDISEASWKLNNYEFYKTVETKKTKSEIFTLKNKKEY" }, "paralog": false, "identity": 0.8976377952755905, "score": 223.0, "evalue": 9.13e-77, "description": "Internal stop codon at 7920, 8130, 8160, 8184. Frameshift due to insertion around 8226. Nonsense mutation around 7920, 8130, 8160, 8184.", "psc": { "uniref90_id": "UniRef90_UPI0021806FCC", "product": "DNA cytosine methyltransferase", "uniref50_id": "UniRef50_UPI0021806FCC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0021806FCC", "UniRef:UniRef50_UPI0021806FCC" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0021806FCC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021806FCC" ], "product": "DNA cytosine methyltransferase" } }, "id": "OAIKOHHJFB_62", "locus": "GCA000209735_00017" }, { "type": "cds", "sequence": "contig_1", "start": 8394, "stop": 8693, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013526934.1", "SO:0001217", "UniParc:UPI0001F5AD4A", "UniRef:UniRef100_A0AB32XBS1", "UniRef:UniRef50_A0AB32XBS1", "UniRef:UniRef90_A0AB32XBS1" ], "nt": "TTGAATGGAATTAAAAATAATACCTATTACTCAAGCTATAACAAAGAATTTAGTGTTAAGCCAACTTGTTTTTATTCAAATATTAATTTAAATTTGAAAAACAAAAGAAAACCAGGCAATGCTGAACACTATTCAAAAGGTAGTTATTATCAACGCTCAATGATTCCAAAAGAATTAATTAAAGATTTATTTTTAAATGTTTTTGTCCAATTGAAAAAAATTTCTGTATTAAGTAAAGAATATACAAAAAACTATATTTCAAATTCACTAGAGAAAGATCAGATCTCCTTATTTGATTAA", "aa": "MNGIKNNTYYSSYNKEFSVKPTCFYSNINLNLKNKRKPGNAEHYSKGSYYQRSMIPKELIKDLFLNVFVQLKKISVLSKEYTKNYISNSLEKDQISLFD", "aa_hexdigest": "156bf1aca29644cfe6b9ec01445bd65c", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F5AD4A", "ncbi_nrp_id": "WP_013526934.1", "uniref100_id": "UniRef100_A0AB32XBS1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5AD4A", "RefSeq:WP_013526934.1", "UniRef:UniRef100_A0AB32XBS1" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XBS1", "uniref90_id": "UniRef90_A0AB32XBS1" }, "psc": { "uniref90_id": "UniRef90_A0AB32XBS1", "uniref50_id": "UniRef50_A0AB32XBS1" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XBS1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XBS1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11665.21419999999, "isoelectric_point": 9.567897987365722 }, "id": "OAIKOHHJFB_63", "locus": "GCA000209735_00018" }, { "type": "cds", "sequence": "contig_1", "start": 9127, "stop": 9471, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTTCTAAAAGAACCTCCTGTTTTTACAGAAGAACAATTTAAAGAATTAGTTCATTATACTGATTTAGAAGATAATCCAACTTTTGAAGCTTACAAATCTAATGCATTTTTCAAAATCTATTCAGATGCTAAAGGTGTTTCAAAATCTTTTAGTAGAATGATTGAAACTGTTTATAGATTAGTGAATGAAAAAAATTTTAATAGTTTTTGGTGTAGAAATCAATATAACCACATTAAAGGAACATTAAAACCATTATTAGAAAAAGTTTTAAGTTTTCTTGCTGCTAAAGGTTTAGATTATAGACCTTATATTAGTATTTACAATACTGAAGCTTATTGA", "aa": "MKFLKEPPVFTEEQFKELVHYTDLEDNPTFEAYKSNAFFKIYSDAKGVSKSFSRMIETVYRLVNEKNFNSFWCRNQYNHIKGTLKPLLEKVLSFLAAKGLDYRPYISIYNTEAY", "aa_hexdigest": "520962d1ecdaad8930985ed86dc5849a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13546.303799999983, "isoelectric_point": 8.573408699035646 }, "id": "OAIKOHHJFB_64", "locus": "GCA000209735_00019" }, { "type": "cds", "sequence": "contig_1", "start": 9790, "stop": 10005, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTCAGGAACAGATTTTTTAAAGAAGATCAATTTAAATTTAGCAAAAGAATTAATTGAAAATACTTATTTGCAAAAAGAAGACTTAGCTTTTAAAGAGGGAATCGGAATTATTGTCACAATGTTTTCTAAATATTTTGTTCCTAAAAATGAATTAAGTGATTTGCAATTAAAACAATTTGAAGCTCTAAAAACTAAAGATTATAAATTATGA", "aa": "MYSGTDFLKKINLNLAKELIENTYLQKEDLAFKEGIGIIVTMFSKYFVPKNELSDLQLKQFEALKTKDYKL", "aa_hexdigest": "69f97ab9b287ff0fef02fd134c9dfc9c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8310.629899999996, "isoelectric_point": 7.8927515029907225 }, "id": "OAIKOHHJFB_65", "locus": "GCA000209735_00020" }, { "type": "cds", "sequence": "contig_1", "start": 10006, "stop": 10098, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTACTGTTGCTGGTTTTGCTTTTGAAGAGCCACCTGAAAGATATGATTATTTTATGAAAAGTGTTTTGCTCAACAAAAATGGCAATTGA", "aa": "MITVAGFAFEEPPERYDYFMKSVLLNKNGN", "aa_hexdigest": "80388edbdc6eb996ef0a3dcc1fa895a4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3481.947400000001, "isoelectric_point": 4.867827796936035 }, "id": "OAIKOHHJFB_66", "locus": "GCA000209735_00021" }, { "type": "cds", "sequence": "contig_1", "start": 10456, "stop": 10557, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGAAGTTTTTATTAACAATGGGATAAATACTAAATTGTTACCTGCTTCAAGATTTAAAGGCAAAAAAGGCTTTGGAATAATTGACAGACAAGTATGA", "aa": "MTEVFINNGINTKLLPASRFKGKKGFGIIDRQV", "aa_hexdigest": "c94b5dbbcc53bf80dbeedb9fc5400956", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3693.3227000000015, "isoelectric_point": 10.446408653259276 }, "id": "OAIKOHHJFB_67", "locus": "GCA000209735_00022" }, { "type": "cds", "sequence": "contig_1", "start": 10790, "stop": 10921, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCAAAACAATTATTAATAAATTAGAAAATGAAAGCAACAAATATTTTAGTTTAAGGTTTAATAATAAACCTGATTCATATAATCAATTTTTAAAATTTGGAACTCAAACAACTATTAACACTATTTGA", "aa": "MSKTIINKLENESNKYFSLRFNNKPDSYNQFLKFGTQTTINTI", "aa_hexdigest": "642a23fba149adc6f0842031d0c11584", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5089.690500000001, "isoelectric_point": 9.601614952087402 }, "id": "OAIKOHHJFB_68", "locus": "GCA000209735_00023" }, { "type": "cds", "sequence": "contig_1", "start": 11054, "stop": 11167, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTGCTCAAGTTTTAGATTATTTGCTTGATAGAAATGGTAACTTGGTAAAACTTAAAACAAGAATCAAATTTTATTCATCAAATATAAGTTTCTATATTGATGAAGAGTGA", "aa": "MPAQVLDYLLDRNGNLVKLKTRIKFYSSNISFYIDEE", "aa_hexdigest": "c5530ee6537ad677a5cf30013a1d86db", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4394.997, "isoelectric_point": 6.101006507873534 }, "id": "OAIKOHHJFB_69", "locus": "GCA000209735_00024" }, { "type": "cds", "sequence": "contig_1", "start": 11459, "stop": 11854, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGTCTTAAAGACATGATCAAACAAGGACTTAAAGATCCTAATGATAGATTTATTCCAATTAATACTGCTATTCAAATGGATGGAACTCCTCCTGTTCAACTTGTGCAAGGCAATAGTCAAGCAATACCATTAATTGAAAAAATAAGATTCAATATTAAACAAATTAAAAAATTTGCTTTCTTAAAAGAAGATTCAAGTGATTCTGGAACTAAAAATATGCATAATGCTGAAGTTCAACAAATTAATAGTGATTTTGAAGATCATATTGAAGCAAAATCAAACTTAAGACAAATGCAACTATTAAACTTTTTCAAAACTTTTGAACCTAGTTTGCTAATTGATAAAGTTTTAATTTTTGGTTCAACTGAATGGCTAAAAAATGAAGCTTGA", "aa": "MNGLKDMIKQGLKDPNDRFIPINTAIQMDGTPPVQLVQGNSQAIPLIEKIRFNIKQIKKFAFLKEDSSDSGTKNMHNAEVQQINSDFEDHIEAKSNLRQMQLLNFFKTFEPSLLIDKVLIFGSTEWLKNEA", "aa_hexdigest": "0dd1a897a9693c93b3eab5c088ec459b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14978.032499999988, "isoelectric_point": 6.10345058441162 }, "id": "OAIKOHHJFB_70", "locus": "GCA000209735_00025" }, { "type": "cds", "sequence": "contig_1", "start": 11949, "stop": 12059, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACTTTATGGAATTAAAATCTTTTTTAGAGCCAAGAACTGGTGAATATATTTTAATAGACAATAAATCACTTTCTTTTGAAAATGGTTCAGTTAATGTAATATCTTGA", "aa": "MDFMELKSFLEPRTGEYILIDNKSLSFENGSVNVIS", "aa_hexdigest": "53b58b1c26164d20f808658d99ee974a", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4124.644200000002, "isoelectric_point": 4.3588346481323255 }, "id": "OAIKOHHJFB_71", "locus": "GCA000209735_00026" }, { "type": "cds", "sequence": "contig_1", "start": 12204, "stop": 12596, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAACTAGATAATAAATTTTATATTTTAGATGCTTTAAATAACCAATATGATTTGGAGCAAAAAAATAATGCTTATTCATATGGAATAGCTCAACCAATTTCTCAAATTGAAAATGAGAAAAAAATAAAATTTGTTTTCATTGAAGAAAAGCCAAATGAAATTAATGTCAGTTTAAGAACTCCATATTTGCACCAAAAAGCTTTAGAAATTGAAAACAATGGTTTAACTAGTGAATTGCCAACAAATGGCCTAGCTTTTGATATGTTTGTTAATCCAATTGAAATTAATGGTATTGACAATAAATACTATTTAAATACTTTGAGCAAATTTTCTCATTTATATTTGAATATTATTTTGAACAAAGAAAATGATTTAATGAAAAACTTTTGA", "aa": "MKLDNKFYILDALNNQYDLEQKNNAYSYGIAQPISQIENEKKIKFVFIEEKPNEINVSLRTPYLHQKALEIENNGLTSELPTNGLAFDMFVNPIEINGIDNKYYLNTLSKFSHLYLNIILNKENDLMKNF", "aa_hexdigest": "050fd94a5c6fe42199a7b8547b95ee1b", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15210.12469999998, "isoelectric_point": 5.154751014709471 }, "id": "OAIKOHHJFB_72", "locus": "GCA000209735_00027" }, { "type": "cds", "sequence": "contig_1", "start": 12648, "stop": 12887, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAATGACTGAAGAAGAAATAAAAAAATTTAAAGAGACTATTTTTGCTGGAAACAATTTAAAAGATTTTGTTGATTTTAAATTTTACACTTTGATTAACTTATATACAAAGAAAGAAAAATTTTTAAAAACTTTATTTGCAATGAGTTCAGGCAATGTTCCTTCAACATATTGTATTGATAATGGTGAAACTGAAAAACATGAACTTTATTTGCCTTTTTATTTTGATAAAAATTGA", "aa": "MGMTEEEIKKFKETIFAGNNLKDFVDFKFYTLINLYTKKEKFLKTLFAMSSGNVPSTYCIDNGETEKHELYLPFYFDKN", "aa_hexdigest": "ca3d6f91fed08138f14434c30ee4989d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9356.634399999995, "isoelectric_point": 5.774637031555175 }, "id": "OAIKOHHJFB_73", "locus": "GCA000209735_00028" }, { "type": "cds", "sequence": "contig_1", "start": 13080, "stop": 13625, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATTGACCAAAAACATGAAGAATTTTTAATGTTTAATAAAGAAAAATTTAATGAAGCTTTTCAATTATCATCAATTCAAACTTCTAGATATACTATGACACCAGAAGAAGCTAGAAAAGAAATTAAAGACTTAGAACCTGGTTTAACTAAAAAGCAAATGTTTGTTCAATTAATTAAAAAAAGCGTTAAAAAAGAAATAAACATTGTTAACGCTTTTGCAAAGGTTCAATTAAACCCAACATATACAGCTAAAATTGCACATGGTGATGAACCTGGAATGATTCATTATACTTATGACACAATTCCTGCTGCATTTAAAATTGTTGATTTAGATGATTTAAATGATTGGTTAGAAGTTGTTAATTTTTCAGGTCTTGATTACAAAAATCTTGACGATAACTATGAATTAATAAATGAAACTTATGTTCCTATTTATACAATTGAATATCAAGTATATGAAAAAGAAGATTTTAAATCAAATATATATGACACTTATTTTAAGTTTCTAAACAATGATTTTATTATCAATAAAAATCAATGA", "aa": "MFIDQKHEEFLMFNKEKFNEAFQLSSIQTSRYTMTPEEARKEIKDLEPGLTKKQMFVQLIKKSVKKEINIVNAFAKVQLNPTYTAKIAHGDEPGMIHYTYDTIPAAFKIVDLDDLNDWLEVVNFSGLDYKNLDDNYELINETYVPIYTIEYQVYEKEDFKSNIYDTYFKFLNNDFIINKNQ", "aa_hexdigest": "e48a24573744bd0463c5248f7d8d07ab", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21492.101900000005, "isoelectric_point": 4.87601261138916 }, "id": "OAIKOHHJFB_74", "locus": "GCA000209735_00029" }, { "type": "cds", "sequence": "contig_1", "start": 13838, "stop": 14161, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDQ6", "UniRef:UniRef90_C4XDQ6" ], "nt": "ATGCATAAAATTATTAGTGTTTTATCTAGCAAACTAGGAATTGAAGAAAGTGAACTCCTTAAAAAACTTTCATTAAATGAAAATTCTTCTCAAAAGGAGATTGCTTCTTCGCTTGGAGTTTATGAGTTTTTCCAAGAAAAAGATGACTTGACTTCATTCATCTTAAATAAAACTCAAAACCAACATAAAGATAATCAAAATTTAAAAACTGAAAATGAAAAGTTAAAAAATGATTATCAAAGTCTTGAGCAACAATTCAACACCAATTTAGAAAATTATAAAAACTTGTCCGGAAAAGTTAAAGATACTTTTAAAAATGAATGA", "aa": "MHKIISVLSSKLGIEESELLKKLSLNENSSQKEIASSLGVYEFFQEKDDLTSFILNKTQNQHKDNQNLKTENEKLKNDYQSLEQQFNTNLENYKNLSGKVKDTFKNE", "aa_hexdigest": "2df2c8230f62cbfa481e4dfd77cc15a9", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 13838, "stop": 14368, "observations": { "mutation": [ 14159, 14342 ], "stop": [ 14159, 14342 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDQ6", "query_cov": 1.0, "subject_cov": 0.6079545454545454, "identity": 0.981, "score": 173.0, "evalue": 1.93e-53, "gene_start": 1, "gene_end": 107, "reference_start": 1, "reference_end": 107, "reference_sequence": "MHKIISVLSSKLGIEESELLKKLSLNENSSQKEIASSLGVYEFFQEKDDLTSFILNKTQNQHKDNQNLKTENEKLKNDYQSLEQQFNTNLENYKNLSGKVKDTFKNEWNKYKLNEIDFEKIDVNSINLNNLFQSVEQVAKENNITIKTKEPNSINNINQDSSSNSSDPWSPYIRNY" }, "paralog": false, "identity": 0.9772727272727273, "score": 315.0, "evalue": 6.83e-112, "description": "Internal stop codon at 14159, 14342. Nonsense mutation around 14159, 14342.", "psc": { "uniref90_id": "UniRef90_C4XDQ6", "uniref50_id": "UniRef50_C4XDQ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDQ6", "UniRef:UniRef50_C4XDQ6" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDQ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDQ6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 12444.701699999987, "isoelectric_point": 5.738146400451661 }, "id": "OAIKOHHJFB_75", "locus": "GCA000209735_00030" }, { "type": "cds", "sequence": "contig_1", "start": 14174, "stop": 14344, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGAAATTGATTTTGAGAAGATAGATGTAAATTCAATTAATCTAAATAATTTGTTTCAAAGTGTTGAGCAAGTGGCAAAAGAAAATAACATAACTATTAAGACTAAAGAACCTAATTCTGTAAATAATATTAACCAAGATTCTAGTTCTAATAGTTCTGATCCATGA", "aa": "MNEIDFEKIDVNSINLNNLFQSVEQVAKENNITIKTKEPNSVNNINQDSSSNSSDP", "aa_hexdigest": "478824230b7a48d6d4a4cf1a37b17b6c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6281.705800000002, "isoelectric_point": 4.207586097717285 }, "id": "OAIKOHHJFB_76", "locus": "GCA000209735_00031" }, { "type": "cds", "sequence": "contig_1", "start": 14381, "stop": 14608, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTTATCAAATACCAACTTTAAAACAATATTTAACTGCTGAATCTGCTACTTTATTTGAACAAGGAGCTGATAAAGCTTATACCATATCAAATGGTGGTCTAACTAAAATTTCAACTTTATTGAATGCAATTTTAGAAACAAACTTTTTAGGTAGTACATTAACTCCTGCAAGTGATCCAAATTCACCAGTTATTGAATATTTAATTTCTTTAGATCAAGAATGA", "aa": "MSYQIPTLKQYLTAESATLFEQGADKAYTISNGGLTKISTLLNAILETNFLGSTLTPASDPNSPVIEYLISLDQE", "aa_hexdigest": "8f6d975d7cc68fc30c5c7462443b62c8", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8113.025099999999, "isoelectric_point": 4.246691322326661 }, "id": "OAIKOHHJFB_77", "locus": "GCA000209735_00032" }, { "type": "cds", "sequence": "contig_1", "start": 14891, "stop": 15112, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTAAAAAAACAGATGAAGAAGTTTATAGTGAAATTGTAAACATGGCATTAAGATTAATGGAACATGAAGATTCAACATTAGGAATTGACGGAATTGAACAAAGCAAAATTGGAATTTTTGTCAAACCAAGTATTTATCAAAAAATAGTCAGATATTCAACACAAGGAAACTTTGTAAAAGAATCAATTCAAGGTAATCCTAAAGACCCAAGAACATGA", "aa": "MTKKTDEEVYSEIVNMALRLMEHEDSTLGIDGIEQSKIGIFVKPSIYQKIVRYSTQGNFVKESIQGNPKDPRT", "aa_hexdigest": "bc0ef6f55afd932b9a9a0218ba8d1c7a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8333.397099999996, "isoelectric_point": 5.746331214904786 }, "id": "OAIKOHHJFB_78", "locus": "GCA000209735_00033" }, { "type": "cds", "sequence": "contig_1", "start": 15278, "stop": 15373, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAATTCAAAACTTTTTTGGAATAGTTTGTGATTCAAACTTTGAAGCTATTAAAAAACCAACAACTGCACCAATTCCAAAACCTGGCTCTTAA", "aa": "MEIQNFFGIVCDSNFEAIKKPTTAPIPKPGS", "aa_hexdigest": "593e187a9ec315ef0a1f70ce5ea08b3f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3380.8853000000013, "isoelectric_point": 5.934922981262207 }, "id": "OAIKOHHJFB_79", "locus": "GCA000209735_00034" }, { "type": "cds", "sequence": "contig_1", "start": 15376, "stop": 15642, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATTTATTCTAACCCAAAACCAAAAACAACCTTGCAATTAAGCCCAAAATCATTTGTTATTAATGATTCAAAAAAACAAATGAATCCTTTTTTGCGAGGTTTTCTTTCATTCCTACATGTTGCTTTGGATATAGGAGCAAGTTTTTTAGGTAGTTTTATTAATACCTTTGCGCCTGGTCTTGGTCTTGTTATTGAATATGGTCTAAGTACTGCCTTAGATTTAGGTTTTAATGCTGCTATAAACTATGGAAATATCAATTGA", "aa": "MLIYSNPKPKTTLQLSPKSFVINDSKKQMNPFLRGFLSFLHVALDIGASFLGSFINTFAPGLGLVIEYGLSTALDLGFNAAINYGNIN", "aa_hexdigest": "babb1ec3af0953af48148397bc63a708", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9554.99199999999, "isoelectric_point": 9.157943534851075 }, "id": "OAIKOHHJFB_80", "locus": "GCA000209735_00035" }, { "type": "cds", "sequence": "contig_1", "start": 15685, "stop": 16167, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF6197B", "UniRef:UniRef90_UPI000FF6197B" ], "nt": "GTGACAAGAGGAATAAGATATGCAGCAAACACACATAAAACTAACAAGTTTTTTCAAATTGCAAAAAATCTTAAAAAAGAGGGTCAAGAAGAATTAAGTGAAAAAATAACAAAAATAGCTGCTAATTATTCTGAATTAGGAGTTGACAAAGCTAAATTTATTGACAATAAATCACTTTTAAAAAAAGAACTAAAATTATTTAATAATGATTCATTTTTGAGAACTTTATCTGATAACAAAAATATTTATTATTTTTTGAATAAAAAGGTTCAAAATATACAAAAAGCTTTCACACATTTAAGATCTATGGTCTCATTATTAACCTCTCCCTCATATTTAGCAAAAAAAGCAGTTGACACTGTATTTAAACACCCAAAAGCCTATTTGAACAAACAACTTGGCAAATTTACCAAATTATTAAAAAATACTTTTAAATCAAAACCAATTAAGAAAGTAGTTAAGAAAGCTTCTAATTATATTTGA", "aa": "MTRGIRYAANTHKTNKFFQIAKNLKKEGQEELSEKITKIAANYSELGVDKAKFIDNKSLLKKELKLFNNDSFLRTLSDNKNIYYFLNKKVQNIQKAFTHLRSMVSLLTSPSYLAKKAVDTVFKHPKAYLNKQLGKFTKLLKNTFKSKPIKKVVKKASNYI", "aa_hexdigest": "f2b10e432bf332c6d2062da8dfad3aa6", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 15385, "stop": 16105, "observations": { "insertion": [ 16039 ], "mutation": [ 15640 ], "stop": [ 15640, 16070 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000FF6197B", "query_cov": 0.8875, "subject_cov": 0.5867768595041323, "identity": 0.8590000000000001, "score": 218.0, "evalue": 3.07e-69, "gene_start": 1, "gene_end": 142, "reference_start": 104, "reference_end": 238, "reference_sequence": "MLIYSNPKPKTTLQLSPKSFVINDSKKQMNPFLRGFLSFLHVALDIGASFLGSFVNTFAPGLGLVIEYGLSTALDLGFNAAINYGNINWQDFGISAGINAIPFVTRGIRYAANTHKTNKFFQLAKNLKKEGQEELSEKITKIAANYSELGVDKAKFIDQKSLLKKELKLFNNDSFLRTLSDNKNIYYFLNKKVQNIQKAFTHLRSMVSLLTSPSYLAKKAVALYLNTQKPIWTNNLANLQNY" }, "paralog": false, "identity": 0.9707112970711297, "score": 451.0, "evalue": 2.11e-163, "description": "Internal stop codon at 15640, 16070. Frameshift due to insertion around 16039. Nonsense mutation around 15640.", "psc": { "uniref90_id": "UniRef90_UPI000FF6197B", "uniref50_id": "UniRef50_UPI000FF6197B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000FF6197B", "UniRef:UniRef50_UPI000FF6197B" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000FF6197B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF6197B" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 18511.5323, "isoelectric_point": 10.1827974319458 }, "id": "OAIKOHHJFB_81", "locus": "GCA000209735_00036" }, { "type": "cds", "sequence": "contig_1", "start": 16336, "stop": 16542, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FEDB9C1", "UniRef:UniRef90_UPI000FEDB9C1" ], "nt": "TTGATTAAATTAGTTTGTGCTAAAAGTCCTGGAAGATACTATTTAAATAATATTGCTTATGGCCATATAGTAGGAAGATTTATTAGAAAATTTGAAATGTTTCAAGCTGTTCCAATACACCCTATTTTCTCCAATTTAATAGGAACTTCATTATTTACTTTTAGAACAATTAATTCTCTTATTATGAATTTTAAAAGACAAAAATGA", "aa": "MIKLVCAKSPGRYYLNNIAYGHIVGRFIRKFEMFQAVPIHPIFSNLIGTSLFTFRTINSLIMNFKRQK", "aa_hexdigest": "bd01a20bf3fc41619262206686a58e9c", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 16336, "stop": 16791, "observations": { "mutation": [ 16540, 16546, 16615, 16660, 16705, 16753, 16765 ], "stop": [ 16540, 16546, 16615, 16660, 16705, 16753, 16765 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000FEDB9C1", "query_cov": 1.0, "subject_cov": 0.4503311258278146, "identity": 1.0, "score": 141.0, "evalue": 1.06e-41, "gene_start": 1, "gene_end": 68, "reference_start": 1, "reference_end": 68, "reference_sequence": "MIKLVCAKSPGRYYLNNIAYGHIVGRFIRKFEMFQAVPIHPIFSNLIGTSLFTFRTINSLIMNFKRQKWVWLEDKNIILNEIVKGVKENVFKGWRAPGFQFISSMARSWATGNVGYQTKAALRWANKKAFTSKTQKTNYWVNHWVKNKYKK" }, "paralog": false, "identity": 0.9470198675496688, "score": 276.0, "evalue": 1.56e-97, "description": "Internal stop codon at 16540, 16546, 16615, 16660, 16705, 16753, 16765. Nonsense mutation around 16540, 16546, 16615, 16660, 16705, 16753, 16765.", "psc": { "uniref90_id": "UniRef90_UPI000FEDB9C1", "uniref50_id": "UniRef50_UPI000FEDB9C1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000FEDB9C1", "UniRef:UniRef50_UPI000FEDB9C1" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000FEDB9C1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FEDB9C1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7952.441199999995, "isoelectric_point": 10.73645191192627 }, "id": "OAIKOHHJFB_82", "locus": "GCA000209735_00037" }, { "type": "cds", "sequence": "contig_1", "start": 16991, "stop": 17248, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTATTTTGTTAAAACTCAAAGTGACTCCAAAGCTATTAAAAATATTATTGGAGAAACAAGATTTGAAAACTTCAAAAAAGCTATTTATTTGGAAATTAAATATAGACTTTCAAGTAAAAGTTTTCCAGAATATTCAAATAGTGTTTTTCTGGTTTCTAATAATATTTCACTTAATGGTGAAACAAGAGATTTTGGTTCATTATCAAAATTAATAAATTATGAATCTATTCAATTATTAACATTTCCAAGTTGA", "aa": "MSYFVKTQSDSKAIKNIIGETRFENFKKAIYLEIKYRLSSKSFPEYSNSVFLVSNNISLNGETRDFGSLSKLINYESIQLLTFPS", "aa_hexdigest": "ae7805db04171dbe811287e695ce2f8f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9788.01729999999, "isoelectric_point": 9.259674644470213 }, "id": "OAIKOHHJFB_83", "locus": "GCA000209735_00038" }, { "type": "cds", "sequence": "contig_1", "start": 17300, "stop": 17503, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATCATTAAAAGAATTTGTTAACAATAAAGATATTGAATTAGAAAACAAAATAAAAAGCTTGGAAGAATATGACAAATCTCAAACTCAAAATAATATTAATCAAGAAGCGCGTATTAAAAATTTAGAATATAAAACTAATGTTATTAAAAGCAAAAGCGAGATACGACTTGAAGACAATGAGGGAAAATTTCATTTATGA", "aa": "MKSLKEFVNNKDIELENKIKSLEEYDKSQTQNNINQEARIKNLEYKTNVIKSKSEIRLEDNEGKFHL", "aa_hexdigest": "b8d6947ecc42db5bd9d1dded588e4115", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7998.901199999998, "isoelectric_point": 6.583569526672363 }, "id": "OAIKOHHJFB_84", "locus": "GCA000209735_00039" }, { "type": "cds", "sequence": "contig_1", "start": 17537, "stop": 17746, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTTATGCATTTCAAAATGAAAGTTTTAAAAAACTAAAAGACATTTTAGACAACTACGAAAATTTAAAGCAAGCTTTTGAATCCTTTAAAATTGCGCAAGAAAACCAAAATACTAAAAATGTCCAAATCAATTCCACACAAGATAATCAAATAAAAGCTTTAGAAGATAAAGTTGCTACTTTAGAAAATACCACATCTAATGTTTGA", "aa": "MLYAFQNESFKKLKDILDNYENLKQAFESFKIAQENQNTKNVQINSTQDNQIKALEDKVATLENTTSNV", "aa_hexdigest": "60efe6ccc3bc9e3f07fae9f50c636036", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8000.782500000001, "isoelectric_point": 4.927224540710449 }, "id": "OAIKOHHJFB_85", "locus": "GCA000209735_00040" }, { "type": "cds", "sequence": "contig_1", "start": 18377, "stop": 18487, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59902", "UniRef:UniRef100_A0AB32XCS4", "UniRef:UniRef50_A0AB32XCS4", "UniRef:UniRef90_A0AB32XCS4" ], "nt": "ATGAAAATTGAAAAAAGAAATTTAGCTAAAAGAATCAAAGAAGCTTCCAAAGCTTTGAAAACAAATACATTAGACAATAAAATTGAACATGATAAGAAAGGAAACAAATAA", "aa": "MKIEKRNLAKRIKEASKALKTNTLDNKIEHDKKGNK", "aa_hexdigest": "b832ac12d69be293f91c2f306a418c97", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59902", "uniref100_id": "UniRef100_A0AB32XCS4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59902", "UniRef:UniRef100_A0AB32XCS4" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCS4", "uniref90_id": "UniRef90_A0AB32XCS4" }, "psc": { "uniref90_id": "UniRef90_A0AB32XCS4", "uniref50_id": "UniRef50_A0AB32XCS4" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XCS4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCS4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4192.8879000000015, "isoelectric_point": 10.170935249328611 }, "id": "OAIKOHHJFB_86", "locus": "GCA000209735_00041" }, { "type": "cds", "sequence": "contig_1", "start": 18487, "stop": 19134, "strand": "+", "frame": 1, "gene": null, "product": "Mem", "db_xrefs": [ "RefSeq:WP_013526945.1", "SO:0001217", "UniParc:UPI00003B49C6", "UniRef:UniRef100_C4XDR0", "UniRef:UniRef50_Q5QGJ3", "UniRef:UniRef90_Q5QGJ3" ], "nt": "ATGAAAGATTTTTTCGCAAACCCAACTAACTTAATTATATTTTTAGGTGGTTTTGTTTCTTTAATAGCTTCTATTACTGGAATCACAACCACTTTCATTAAAACTCATAATTTAAAGCTTCAAAATAATAACCTTAAACAAGAAAATAAAGACATAAGATTTGAACTTCTTCAAACTCAAAAAGAATTGGCTGATAAAAACAATTCACTTTCAAAACTTGAAGTTATGTTTGACAACCAAAGAAAAGAAATTGAAAGACTAAGTGAAGAATTAAAAGCTTTACAAAGTGGCAATGTTGATCCTTTGGTTAAAAAGCTAAAAGAACAAATTGAGAACCTTGTTAATGAAAAGAAACAATTAACTGAACAAGTCTATAATGTTAAAGCTGAATTAAATGAGTCTAAATATCATTCTGGAGCTTATGCAAATGAAATTGTTGAATTGAAAAGGGAAAATAGTGAGCTTAAAGAGCAATTAAAGCTTGAAAGAGCTAAGATTGTTGAAGACATAGCAAATATATTAACTCCCAAAAACAATGAGGCAAATAAGGCTTCTAATGAAGCAATAAAGGAAAATAAAATAGAAGCCCCAAAAGCAGAAACCTTTGAAGCTCCAAAAAATAATGAAAATGTTAGCCTATATATTTAA", "aa": "MKDFFANPTNLIIFLGGFVSLIASITGITTTFIKTHNLKLQNNNLKQENKDIRFELLQTQKELADKNNSLSKLEVMFDNQRKEIERLSEELKALQSGNVDPLVKKLKEQIENLVNEKKQLTEQVYNVKAELNESKYHSGAYANEIVELKRENSELKEQLKLERAKIVEDIANILTPKNNEANKASNEAIKENKIEAPKAETFEAPKNNENVSLYI", "aa_hexdigest": "e970ed109eecf36c29fe7895627855cd", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00003B49C6", "ncbi_nrp_id": "WP_013526945.1", "uniref100_id": "UniRef100_C4XDR0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00003B49C6", "RefSeq:WP_013526945.1", "UniRef:UniRef100_C4XDR0" ] }, "ips": { "uniref100_id": "UniRef100_C4XDR0", "uniref90_id": "UniRef90_Q5QGJ3" }, "psc": { "uniref90_id": "UniRef90_Q5QGJ3", "product": "Mem", "uniref50_id": "UniRef50_Q5QGJ3" }, "pscc": { "uniref50_id": "UniRef50_Q5QGJ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5QGJ3" ], "product": "Mem" }, "genes": [], "id": "OAIKOHHJFB_87", "locus": "GCA000209735_00042" }, { "type": "cds", "sequence": "contig_1", "start": 19194, "stop": 19568, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAGACACTTCTTTATTGTTAATGCTCATTTAAACATTGGAAGAACAGAAGAAGTTGCAAAGCTTGTTGGTCATGTTAACTCACAAACAACAGCAACAACATATTTAAACTTTAGCAATGTTGCTCTTGAAAATATTATGGAACAAGCTCAAATGGAAAATGAAAGTGCTTTTGACTATTCAAAAATTAAAATTAAATATAAAGAACTAAGCGAAGAAAATGCTCAACTCAAATTAACAATTAGTGAACTAAACAACCAATTAGACAAATACAAAAAAGTTTTAGGTGAAACAAAAGAAAATGTTAATTTTACAGAAGTATTAAATAGCAATATAAGTAAATTAAAAAATTTAATTAAAAATATTAAGTAA", "aa": "MLRHFFIVNAHLNIGRTEEVAKLVGHVNSQTTATTYLNFSNVALENIMEQAQMENESAFDYSKIKIKYKELSEENAQLKLTISELNNQLDKYKKVLGETKENVNFTEVLNSNISKLKNLIKNIK", "aa_hexdigest": "556fdf48c55ed646aa196dd5a0660b85", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14263.132599999979, "isoelectric_point": 7.916024589538574 }, "id": "OAIKOHHJFB_88", "locus": "GCA000209735_00043" }, { "type": "cds", "sequence": "contig_1", "start": 19815, "stop": 19937, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGAATATTTAAAAATATTCAATTATGTTGAATTATGGGGAGATCTTAAATTCAAATTAGTATTCCAAATTTTAGCTATTACAGGAGTTAGAATTGGAGAACTTGCAAATGTTAATTGA", "aa": "MEEYLKIFNYVELWGDLKFKLVFQILAITGVRIGELANVN", "aa_hexdigest": "c802affec5a2eff5b9b4a496be02debb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4688.4856, "isoelectric_point": 5.002479362487793 }, "id": "OAIKOHHJFB_89", "locus": "GCA000209735_00044" }, { "type": "cds", "sequence": "contig_1", "start": 20079, "stop": 20285, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTACAAAAATAAAATTGTTTTAAATAAAGAAATTGACTATGTTAAAGTTTACTTAGATCAATTTAAAATCTTAGTTGAAAGAAAACAAAAAAGTAAAAAAACTTTTCAACATTATTTAACTTTATTGAAAAGACTTAAAAGATTTGGATCGGTTGAAGAATTACTAGACCAAATGAATCATTATTTAGCTTCTTCAAATTGA", "aa": "MFYKNKIVLNKEIDYVKVYLDQFKILVERKQKSKKTFQHYLTLLKRLKRFGSVEELLDQMNHYLASSN", "aa_hexdigest": "14ae6d86cf0451ed63e4c3bddb6dc20a", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8313.694899999997, "isoelectric_point": 9.776324272155762 }, "id": "OAIKOHHJFB_90", "locus": "GCA000209735_00045" }, { "type": "cds", "sequence": "contig_1", "start": 20355, "stop": 20504, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001004FEA6", "UniRef:UniRef90_UPI001004FEA6" ], "nt": "ATGTTTGAAAAAATAAAAATGAACAGATGGCGTTGCCGTTGTTGTCAAACATTAACAAATGACAAAAAAAGAGAAGTTTTTAATTACAAAAATGAAGAATATGTGTTCCCTTATTTTTGTCAAAAATGTTGTCAAAGATTAATTAATTGA", "aa": "MFEKIKMNRWRCRCCQTLTNDKKREVFNYKNEEYVFPYFCQKCCQRLIN", "aa_hexdigest": "c145cb3c93868780b3d52f69aada1530", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI001004FEA6", "query_cov": 1.0, "subject_cov": 0.8909090909090909, "identity": 0.98, "score": 112.0, "evalue": 1.11e-31, "valid": true, "uniref50_id": "UniRef50_UPI001004FEA6" }, "pscc": { "uniref50_id": "UniRef50_UPI001004FEA6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001004FEA6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6250.3111, "isoelectric_point": 9.21532039642334 }, "id": "OAIKOHHJFB_91", "locus": "GCA000209735_00046" }, { "type": "cds", "sequence": "contig_1", "start": 21153, "stop": 21248, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATAAGTGAAAAAGAATATTTTGAAAGAAATGGTATGAAATTTCTAAAACAAACAGAACAAAGAATAGATATTGTTGAAGAAAGATTATGA", "aa": "MLISEKEYFERNGMKFLKQTEQRIDIVEERL", "aa_hexdigest": "29fdb8ea267d583168c0458b7e0f4927", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3874.4435999999996, "isoelectric_point": 5.189195442199705 }, "id": "OAIKOHHJFB_92", "locus": "GCA000209735_00047" }, { "type": "cds", "sequence": "contig_1", "start": 21764, "stop": 21964, "strand": "+", "frame": 2, "gene": null, "product": "Archaeal holliday junction resolvase (hjc)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF36", "UniRef:UniRef90_C4XF36" ], "nt": "TTGTCTATTCTAAAAGCTAAAGGTTACCAAGCTAAGAGAACAAATCTTAAAATGGATGGCATATATAAAATTGATATAGTGGCAACAAAAGGAAATGAGAAGATTTATATTCAAGTTAAGTTAAACAAACCAAAAGCTTTAGAATTGAAGAATTTGCATTCATTCTTAAGATCTAAGGCCTTTCAAGGACTAATTGCATGA", "aa": "MSILKAKGYQAKRTNLKMDGIYKIDIVATKGNEKIYIQVKLNKPKALELKNLHSFLRSKAFQGLIA", "aa_hexdigest": "0f7b71e56878e69c8b68c51f46a7ce5f", "start_type": "TTG", "rbs_motif": "AGxAG", "genes": [], "truncated": "both", "pseudogene": { "start": 21503, "stop": 22000, "observations": { "mutation": [ 21617, 21962, 21980 ], "stop": [ 21617, 21962, 21980 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF36", "query_cov": 1.0, "subject_cov": 0.4, "identity": 0.985, "score": 125.0, "evalue": 1.78e-35, "gene_start": 1, "gene_end": 66, "reference_start": 88, "reference_end": 153, "reference_sequence": "MYENFSRYNLTMKMPNYKDLNFNIKRELNKLKPKSFEEWKEAKSKVFCLALGKYLTKINSHQPDIFKNYYQVLFYHSFINYKREEELLSILKAKGYQAKRTNLKMDGIYKIDIVATKGNEKIYIQVKLNKPKALELKNLHSFLRSKAFQGLIAWKESTEWKFKEI" }, "paralog": false, "identity": 0.9696969696969697, "score": 317.0, "evalue": 1.76e-113, "description": "Internal stop codon at 21617, 21962, 21980. Nonsense mutation around 21617, 21962, 21980.", "psc": { "uniref90_id": "UniRef90_C4XF36", "product": "Archaeal holliday junction resolvase (hjc)", "uniref50_id": "UniRef50_C4XF36", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF36", "UniRef:UniRef50_C4XF36" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF36", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF36" ] } }, "id": "OAIKOHHJFB_93", "locus": "GCA000209735_00048" }, { "type": "cds", "sequence": "contig_1", "start": 21987, "stop": 22565, "strand": "-", "frame": 3, "gene": null, "product": "Type I restriction modification DNA specificity domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDR5", "UniRef:UniRef90_C4XDR5" ], "nt": "GTGTCAAAGGAAAATGGTATAAGAATATTAAGAATATCTGATTTTGATGAACGTGGTTTAAAAAATAACAATATTGTTTATTATAAATATGAAAGTAAAATGTTTGATTATTTTTTAAATAATAAAAACATTGTTATTTGTATGACAGGTGGAACAGTTGGTAAATCTTTATTAATTAAAGAATTAAAAGAAAAAATTCTTGTAAATCAAAGAGTTGGCAATATAAAAATATTAAATGAAATGTTACCTGATTATGTAGATATTGTTATGAAAAGTGAACTAATATCAAAAATTATCAGAAAAAATAAAAATTCTACAAATGATAATATTTCAATAGAGCTCATAAAATTGTTTTTTATTCCAGTTCCCTCTATTGAAGAACAATTAAAAATTATAGTTAAATATAACAAATTGTTAACACAATTGACATTATATAAAAATATATTCCCTTATATATTCCTACTATATATTCCCGTCAATATCTGGTATTTAAGATTTTTAGTGAACAAAACAATTCACTTTTCAACTTTATTTTATATCAAAATTTTAAATATGTTAAAAAATGTTAAATTTCTTTGA", "aa": "MSKENGIRILRISDFDERGLKNNNIVYYKYESKMFDYFLNNKNIVICMTGGTVGKSLLIKELKEKILVNQRVGNIKILNEMLPDYVDIVMKSELISKIIRKNKNSTNDNISIELIKLFFIPVPSIEEQLKIIVKYNKLLTQLTLYKNIFPYIFLLYIPVNIWYLRFLVNKTIHFSTLFYIKILNMLKNVKFL", "aa_hexdigest": "4ca6e3ba34f1e5c32a8d4d0bd41e49bb", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 21936, "stop": 22865, "observations": { "mutation": [ 21989, 22628, 22634 ], "stop": [ 21989, 22628, 22634 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDR5", "query_cov": 1.0, "subject_cov": 0.5907692307692308, "identity": 0.9840000000000001, "score": 355.0, "evalue": 1.36e-121, "gene_start": 1, "gene_end": 192, "reference_start": 117, "reference_end": 308, "reference_sequence": "MKINRLTAEQLRKSILQLAIQGKLVKQDPNDEPASKLIERIYQEKQKLIKEGKIKKDKQESFIFKGEDNHYYEKIGKNIKDITEELPFEIPENWMWVRLKNISIINGGFAFKSSEFVSKENGIRILRISDFDERGLKNNNIVYYKYESKMFDYFLNNKNIVICMTGGTVGKSLLIKELKEKILVNQRVGNIKILNEMLPDYVDIVMKSELISKIIRKNKNSTNDNISIELIKLFFIPVPSIEEQLKIIVKYNKLLTQLTLYKNIFLYIFPLYIPVNIWYLRFLVNKTIHFSTLFYIKILNMLKNVKFLWIFTQLTLFMQLVLERP" }, "paralog": false, "identity": 0.9838187702265372, "score": 590.0, "evalue": 8.27e-217, "description": "Internal stop codon at 21989, 22628, 22634. Nonsense mutation around 21989, 22628, 22634.", "psc": { "uniref90_id": "UniRef90_C4XDR5", "product": "Type I restriction modification DNA specificity domain-containing protein", "uniref50_id": "UniRef50_C4XDR5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDR5", "UniRef:UniRef50_C4XDR5" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDR5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDR5" ], "product": "Type I restriction modification DNA specificity domain-containing protein" } }, "id": "OAIKOHHJFB_94", "locus": "GCA000209735_00049" }, { "type": "cds", "sequence": "contig_1", "start": 22632, "stop": 22913, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGATTAATAGACTTACAGCAGAACAATTGAGAAAATCTATTCTTCAATTGGCTATTCAAGGAAAATTAGTTAAACAAGATCCAAATGATGAGCCTGCTAGCAAACTTATTGAGAGAATTTATCAAGAAAAACAAAAATTAATTAAAGAAGGAAAAATAAAGAAAGATAAACAAGAATCTTTCATTTTTAAAGGTGAGGATAATCATTATTATGAGAAGATAGGCAAAAATATTAAAGATATTACTGAAGAATTGCCTTTTGAAATACCAGAGAATTGA", "aa": "MKINRLTAEQLRKSILQLAIQGKLVKQDPNDEPASKLIERIYQEKQKLIKEGKIKKDKQESFIFKGEDNHYYEKIGKNIKDITEELPFEIPEN", "aa_hexdigest": "086a9e3314cbc420a472e1686bec0810", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI002ED51773", "query_cov": 0.9247311827956989, "subject_cov": 1.0, "identity": 0.628, "score": 103.0, "evalue": 3.61e-27, "valid": false, "uniref50_id": "UniRef50_K1U594" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10984.550299999992, "isoelectric_point": 8.752953147888185 }, "id": "OAIKOHHJFB_95", "locus": "GCA000209735_00050" }, { "type": "cds", "sequence": "contig_1", "start": 23227, "stop": 24012, "strand": "-", "frame": 2, "gene": null, "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ1V4Q7" ], "nt": "ATGAAGGACGGTGTTTTATTAAGACAAGTTATTAATGTAATTAATGAATTAGATTTTGCTGACTATAAAGAAAAACATGAGTTTGGTACTATATATGAAACTATTTTAAAAAGCTTGCAAAGTGCTGGAAATGCAGGTGAATTTTATACACCAAGAGCAGTAACTGATTTTATGGTTAAAATGATTAATCCAAAATTAGGAGAAAAAATTGCAGATTTTGCTTGCGGAACAGGTGGATTCTTAACATCTTCATTGAAGCATTTAGAGGATCAAAAGAAAACAGTTGAAGATGAAAACTTATATGATAATTCAGTCTATGGCATTGAGAAAAAACCATTGCCATATCTTTTATGTATTACTAATATGTTAATTCATGATGTAGATGAACCAAAAATATTTCATGATAATTCTTTAGAAAAAAGAGTACAAGATTATACTGAAGCTGATAAATTCGACATTATCTTAATGAATCCCCCATATGGTGGTTCAGAAAAAGAAGCTGTAAAAAATAATTTTCCAGCTGATTTAAGAAGTAGTGAAACTGCAGATTTATTTATGAACGTTATTATGTATCGTTTAAAGAAAAAAGGACGTTGTGCAGTTATATTGCCTGATGGTTTTTTATTTGGAGCAGATAATGCAAAAGTTGCAATAAAAACTAAATTATTAAAAGAATTTAATTTACACACCATTATTAGAATGCCGCATAGTGTTTTTGCTCCATATACTTCAATAACAACAAATATTTTGTTTTTTGATCACACTCATCCAACTGAAGAAGTTTGA", "aa": "MKDGVLLRQVINVINELDFADYKEKHEFGTIYETILKSLQSAGNAGEFYTPRAVTDFMVKMINPKLGEKIADFACGTGGFLTSSLKHLEDQKKTVEDENLYDNSVYGIEKKPLPYLLCITNMLIHDVDEPKIFHDNSLEKRVQDYTEADKFDIILMNPPYGGSEKEAVKNNFPADLRSSETADLFMNVIMYRLKKKGRCAVILPDGFLFGADNAKVAIKTKLLKEFNLHTIIRMPHSVFAPYTSITTNILFFDHTHPTEEV", "aa_hexdigest": "5ac17f212597497eccde84c953af62dc", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003461D892", "query_cov": 0.8275862068965517, "subject_cov": 1.0, "identity": 0.6940000000000001, "score": 325.0, "evalue": 3.53e-110, "valid": false, "product": "class I SAM-dependent DNA methyltransferase", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0AAJ1V4Q7", "kegg_orthology_id": "K03427" }, "pscc": { "uniref50_id": "UniRef50_A0AAJ1V4Q7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ1V4Q7" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "OAIKOHHJFB_96", "locus": "GCA000209735_00051" }, { "type": "cds", "sequence": "contig_1", "start": 24310, "stop": 24405, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATTGGAAATTTAATTAAAAGAATTCAAGATATTATGAGAAACGATGCAGGTGTTGATGGCGATGCTCAACGTATTAGTCAAATGACTTGA", "aa": "MAIGNLIKRIQDIMRNDAGVDGDAQRISQMT", "aa_hexdigest": "0be4c54aad361adb3bac644183bc58d7", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3431.9204999999993, "isoelectric_point": 5.854097938537596 }, "id": "OAIKOHHJFB_97", "locus": "GCA000209735_00052" }, { "type": "cds", "sequence": "contig_1", "start": 24424, "stop": 24786, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCAAAGGCATTTTAATTGAAAACTTAAGAAATATTTATCCAAGAGATTTTGATGATTTCGATTTAATCTGTAGCATTGCATATGGATCAAAATTACTAAAAAGATCTGAAAGAGTAAATAATAAAAAAGTTGTTCAAATAATAGAAAGACAAAATGTTGAAACATGTAGAAAAATTTTACAAATTCTTATAAATAAATATCAAGAAGAAGTCATTGATGAATTAACAGATAACAATAATTTGAGATTAAAAGAATTTGAACAATTTGGCGGTCCTTTAAAAATTGCTAAACTTTTTGGTGGAAAAGAACAATATATTCAGACTGTTAAAGAAATTCAAAAAGCACTTTATATAGTATAA", "aa": "MSKGILIENLRNIYPRDFDDFDLICSIAYGSKLLKRSERVNNKKVVQIIERQNVETCRKILQILINKYQEEVIDELTDNNNLRLKEFEQFGGPLKIAKLFGGKEQYIQTVKEIQKALYIV", "aa_hexdigest": "a621653482aa9d1c3d44a020045661ab", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14093.229899999988, "isoelectric_point": 8.847399330139162 }, "id": "OAIKOHHJFB_98", "locus": "GCA000209735_00053" }, { "type": "cds", "sequence": "contig_1", "start": 25021, "stop": 25662, "strand": "-", "frame": 2, "gene": null, "product": "Type I restriction-modification enzyme R subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A377ZNK3" ], "nt": "GTGGAAGACAATGAATACAATGTAAAGGATTTTGATAAAACTATTGTAATAGAAGAAAGAACAAAGGAGGTAGCTAAAAAAATTACCGATTATTTAAAATCAATTAACAATAGATATGCAAAAACAATTGTATTTTGTGACGACATAGAACATGCTGAGAGAATGAGACAAGCTTTAGTTCACTACAATAGTGATATGATGGCCAAAGATCATAGATATATTGTTCGTATTACTGGTGACAATGAAGAAGGTAAAAAACAATTAGATAATTTCATTGATGATAATGAGCTATATCCAACAATAGTTACAACCAGTGAACTTATGACTACAGGTGTCAATTGTAAATTGTGTCGTGTTATTGCACTTGATAATACTTTTGGTGAAAATGGTATGACTAAATTTAAACAAATAATTGGCCGAGGCACCAGAATTAAAGAATTTGATGATCCTAAAGATAAACATAAAAACAAATTGTATTTCACTATTTTAGACTTTAAAAATGCTACTAAATTATTTGCTGATCCTGATTTTAATGGCCCAGCAATCCAAGAAGAATTTTTTAATCCTGAAATTCTTAGAAATAAAAAAGCAAAACAAGAAACAAAAAAAGAATATGAATTAGTTGATGAGGGATTAGTCTGA", "aa": "MEDNEYNVKDFDKTIVIEERTKEVAKKITDYLKSINNRYAKTIVFCDDIEHAERMRQALVHYNSDMMAKDHRYIVRITGDNEEGKKQLDNFIDDNELYPTIVTTSELMTTGVNCKLCRVIALDNTFGENGMTKFKQIIGRGTRIKEFDDPKDKHKNKLYFTILDFKNATKLFADPDFNGPAIQEEFFNPEILRNKKAKQETKKEYELVDEGLV", "aa_hexdigest": "a5e57a0eeff3a2d57b4c593c32ad93f3", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A377ZNK3", "query_cov": 0.8544600938967136, "subject_cov": 0.9479166666666666, "identity": 0.56, "score": 189.0, "evalue": 1.55e-57, "valid": false, "product": "Type I restriction-modification enzyme R subunit", "uniref50_id": "UniRef50_A0A377ZNK3" }, "pscc": { "uniref50_id": "UniRef50_A0A377ZNK3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A377ZNK3" ], "product": "Type I restriction-modification enzyme R subunit" }, "genes": [], "id": "OAIKOHHJFB_99", "locus": "GCA000209735_00054" }, { "type": "cds", "sequence": "contig_1", "start": 25900, "stop": 26136, "strand": "-", "frame": 2, "gene": null, "product": "DEAD/DEAH box helicase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0031B703C8" ], "nt": "TTGATTGATCAAACTATAATAGGGGATTTTAAACCTTTCAAAAATAGTATGGTTAAAGTTTCAAAAAGAAATATGGACACATCTTATGAAATTTATTTATCTCTATATCAACAATTATCTGAAAATGGCTCCGAAGATTCACTCAAAAAATTAAAAGAAAGCTTTAAACCAGACTTTTTCGATTTAATAATTGTTGATGAATGTCACAGAGGTAGTGCAAGTGAAGATTCTAAATGA", "aa": "MIDQTIIGDFKPFKNSMVKVSKRNMDTSYEIYLSLYQQLSENGSEDSLKKLKESFKPDFFDLIIVDECHRGSASEDSK", "aa_hexdigest": "595984c7b02e00fba0ae456d81d14e2e", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00300492D9", "query_cov": 0.9871794871794872, "subject_cov": 0.9058823529411765, "identity": 0.5579999999999999, "score": 91.3, "evalue": 1.45e-22, "valid": false, "product": "DEAD/DEAH box helicase family protein", "uniref50_id": "UniRef50_UPI0031B703C8" }, "pscc": { "uniref50_id": "UniRef50_UPI0031B703C8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0031B703C8" ], "product": "DEAD/DEAH box helicase family protein" }, "genes": [], "id": "OAIKOHHJFB_100", "locus": "GCA000209735_00055" }, { "type": "cds", "sequence": "contig_1", "start": 26185, "stop": 26715, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13588.12" ], "nt": "ATGGAATATTATATTTCTGCTGGAAAAGTGATTTTCGAAAATGGTGAGGCTAAAAAACTAAAAGCAAAAAGAATTGATTATTTGTTGAATTACAATGGAGATGGTTCACCGCTCGCAATTATTGAAGCAAAAGGTGAAGATCATAAAGAAGGCGACGGATTACTCCAAGCTCAAAATTATGCTAAAAGGTTGGGCGTTCGTTTTGTTTTTACATCAAATGGTAACAATTTTCGATTTTATGATATGAAAACAGGAATAGAGAAAACAATAGGGTTAGATGAATTTTTCACGCCCGATGAATTATTTGAGATGACATATGGTGAAGAAATCAAGGAAAATAAGGCTTTAGAAAAAATAATTTCCACTCCTTATTATTATGGACAAAATTCTCATATTCCTAGATATTATCAAAAAAGAGCCATAAATGATACAGTAGACGCTGTTGCTAAAGGAAAAGATAGAATTTTACTAGTTATGGCCACTGGCACAGGTAAAACATATGTTGCTTTTCAAATAATCTGGAGATTGTGA", "aa": "MEYYISAGKVIFENGEAKKLKAKRIDYLLNYNGDGSPLAIIEAKGEDHKEGDGLLQAQNYAKRLGVRFVFTSNGNNFRFYDMKTGIEKTIGLDEFFTPDELFEMTYGEEIKENKALEKIISTPYYYGQNSHIPRYYQKRAINDTVDAVAKGKDRILLVMATGTGKTYVAFQIIWRL", "aa_hexdigest": "dc17bafc6ae91c0dfe2829e24a1fbdf1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13588.12", "name": "HSDR_N_2", "length": 76, "aa_cov": 0.42613636363636365, "hmm_cov": 0.9868421052631579, "evalue": 1.406618040910839e-08, "score": 30.914335250854492, "start": 20, "stop": 94 } ], "seq_stats": { "molecular_weight": 20151.709899999994, "isoelectric_point": 7.782317161560059 }, "id": "OAIKOHHJFB_101", "locus": "GCA000209735_00056" }, { "type": "cds", "sequence": "contig_1", "start": 27112, "stop": 27444, "strand": "+", "frame": 1, "gene": null, "product": "protein adenylyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6G5QHC3" ], "nt": "ATGAAAAATTCAATAGATCAAAAAAGTAAAATAAAGGCTTATAACTTATTTGACTTAATAATAGAAGATAAATTAGAAATAGGCACCACTAAAAGTTTAATGCAAATTCATGGTTATTTATTTGGTGGTCTTTATTCTTTTGCTGGTCAAATTAGAAAAAAAGATATTTCAAAAGGTGGCTTCAGATTTGCTAATTCTACATATTTAAATGAAACACTTAAAAATATTGAAAATATGCCCGAAAGTAATTTGGAGCAAATAATAGATAAATATATTGAAATGAATATAGCGCATCCATTTCTAGAAGGCAATGGACGAAGTACTAGAATTTGA", "aa": "MKNSIDQKSKIKAYNLFDLIIEDKLEIGTTKSLMQIHGYLFGGLYSFAGQIRKKDISKGGFRFANSTYLNETLKNIENMPESNLEQIIDKYIEMNIAHPFLEGNGRSTRI", "aa_hexdigest": "973984e6cb6815f6a28c7ffd5ddc9218", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A6G5QHC3", "query_cov": 1.009090909090909, "subject_cov": 0.8473282442748091, "identity": 0.613, "score": 132.0, "evalue": 7.2e-38, "valid": false, "product": "protein adenylyltransferase", "uniref50_id": "UniRef50_A0A6G5QHC3" }, "pscc": { "uniref50_id": "UniRef50_A0A6G5QHC3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6G5QHC3" ], "product": "protein adenylyltransferase" }, "genes": [], "id": "OAIKOHHJFB_102", "locus": "GCA000209735_00057" }, { "type": "cds", "sequence": "contig_1", "start": 27523, "stop": 27648, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A134CFU1" ], "nt": "ATGCAAGAGAGCCCTTATGATGATACAAAAATTAAAAATTTAATTAAAAAATCATTAACCGATAAGATTAATGATCGCGAATTATTTATGAAGGGGATTGATTATTCTTACTACTATGAAGAGTAA", "aa": "MQESPYDDTKIKNLIKKSLTDKINDRELFMKGIDYSYYYEE", "aa_hexdigest": "c7979606339603708649a7e1e6577ae1", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A134CFU1", "query_cov": 1.0, "subject_cov": 0.803921568627451, "identity": 0.585, "score": 48.5, "evalue": 1.11e-06, "valid": false, "uniref50_id": "UniRef50_A0A134CFU1" }, "pscc": { "uniref50_id": "UniRef50_A0A134CFU1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A134CFU1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5026.603400000001, "isoelectric_point": 4.920460700988768 }, "id": "OAIKOHHJFB_103", "locus": "GCA000209735_00058" }, { "type": "cds", "sequence": "contig_1", "start": 28021, "stop": 28254, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAAATTAAACCTAAGGAATTATTCGACAAAGTTAAAGAAGCAATTAAAAATAAAGAAGTAGGTGACAAAAGTGACTATAGATATAAACTAAAAGGTAATTTAGCAAATATTAAATATGCCAGATTATATAAAGATTTAATGGATATAAATAATATGATTCTTGCTAATTTATGTGATTTAGTTAGAAATGAAGATATATCTAAAAGTTCTTTAGGTGGTAAACCTTGA", "aa": "MKEIKPKELFDKVKEAIKNKEVGDKSDYRYKLKGNLANIKYARLYKDLMDINNMILANLCDLVRNEDISKSSLGGKP", "aa_hexdigest": "fb181170c2c7abb9c6fc525910557e75", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8877.295499999995, "isoelectric_point": 9.35205783843994 }, "id": "OAIKOHHJFB_104", "locus": "GCA000209735_00059" }, { "type": "cds", "sequence": "contig_1", "start": 28735, "stop": 28923, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACTATTTAATATTATAACTTCAGATGACATTAATGATGAAGTTGGTTGCGACATCATGGAAGAATATGAATTAGAAATGTATGAAGAAGTTAGAAATTATATAAAACGCGAAATAGGCAACAAAAAAACATCTAAAGTAGTGGCTTATCAACTTTGTGATTCAAAAGGTAAAAGTATTTGA", "aa": "MKKLFNIITSDDINDEVGCDIMEEYELEMYEEVRNYIKREIGNKKTSKVVAYQLCDSKGKSI", "aa_hexdigest": "a31bd328f002aa5802a348a92729451f", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7269.242900000002, "isoelectric_point": 4.850491905212402 }, "id": "OAIKOHHJFB_105", "locus": "GCA000209735_00060" }, { "type": "cds", "sequence": "contig_1", "start": 29251, "stop": 29853, "strand": "+", "frame": 1, "gene": null, "product": "HTH rpiR-type domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDS0", "UniRef:UniRef90_C4XDS0" ], "nt": "TTGCAAATTTATGTTGCACGTAAAGTGGAGCAATTTTTACCAAACGAAACCTATAAAATTGATGAAATAAAAGATGCAAAAGATTGTATTTTAAAAGTTTTTCAACAATATCAAAATATTTCAAAAGCAGGTTTTAATACAATAGAAACTATTCAAATTGAAAAACTTGTTAATAATTTAATCAAATACAAAAAGTTAATTATTTTTGGAATAGGTACTAGTGGAATTATAGGTTCTTATTTAGCCAAGCAATTAACAAGAATTGGAATAATTTCTGTATGTATAAATTCGATCCATGATTTTAAGGATGCATACAATGAATCAAATAAAGATGATGTTTTTTATATCATTATTTCTAAATCATTTAAAAATCATGAAGTTAAAGAAGCTGCAGAAATACTAGATAAAAGCAATTTTAATTTTCAAATATTGACAAGAAACGAAAATGTTTCTTATGCAAATTGCACCTCACCAATAATTTATGATTATATTCCTGCACAAAAAAACTTTAATTATGATATTGTGTCAAAATTGTCTTTATTTATGCTCATAGATATAATTTATATCTACGCCAAAACCTTAATAGACAATAAAAATAAGTAA", "aa": "MQIYVARKVEQFLPNETYKIDEIKDAKDCILKVFQQYQNISKAGFNTIETIQIEKLVNNLIKYKKLIIFGIGTSGIIGSYLAKQLTRIGIISVCINSIHDFKDAYNESNKDDVFYIIISKSFKNHEVKEAAEILDKSNFNFQILTRNENVSYANCTSPIIYDYIPAQKNFNYDIVSKLSLFMLIDIIYIYAKTLIDNKNK", "aa_hexdigest": "226807ccab47cad368ce4d053dd09b1e", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 29050, "stop": 29853, "observations": { "mutation": [ 29125 ], "stop": [ 29125 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDS0", "query_cov": 1.0, "subject_cov": 0.7490636704119851, "identity": 0.995, "score": 389.0, "evalue": 1.01e-135, "gene_start": 1, "gene_end": 200, "reference_start": 68, "reference_end": 267, "reference_sequence": "MMKRIKIFDKKLVENYKDTYSYLIKWIEENPHEFLACSQHQITEKIYVSQSSLSRFTKFLGFESYRELQIYVARKVEQFLPNETYKIDEIKDAKDCILKVFQQYQNISKAGFNTIETIQIEKLVNNLIKYKKLIIFGIGTSGIIGSYLAKQLTRIGIISVCINSIHDFKDAYNESNKDDVFYIIISKSFKNHEVKEAAEILDKSNFNFQILTRNENVSYANCTSPIIYDYIPAQKNFNYDIVSKLSLFMLIDIIYIYAKTLIDNKNK" }, "paralog": false, "identity": 0.9925093632958801, "score": 525.0, "evalue": 1.44e-191, "description": "Internal stop codon at 29125. Nonsense mutation around 29125.", "psc": { "uniref90_id": "UniRef90_C4XDS0", "product": "HTH rpiR-type domain-containing protein", "uniref50_id": "UniRef50_C4XDS0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDS0", "UniRef:UniRef50_C4XDS0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDS0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDS0" ], "product": "HTH rpiR-type domain-containing protein" } }, "id": "OAIKOHHJFB_106", "locus": "GCA000209735_00061" }, { "type": "cds", "sequence": "contig_1", "start": 30007, "stop": 30168, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAGAAAAATTAGTTAAATACTTAAAAATGACTATTGTTATTTTCTTAGCTGGTAGCCTAATTGGAATTTATTTTCTAAAAACAGGTCAATTTGAAAATCATAGTCAAAAAATACTTTTAGATAGATTAAGTAATAATTACAACCGTAATTTTGCTTGA", "aa": "MKEKLVKYLKMTIVIFLAGSLIGIYFLKTGQFENHSQKILLDRLSNNYNRNFA", "aa_hexdigest": "a38f002e3941c1695047e633cfac5783", "start_type": "GTG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6223.3135999999995, "isoelectric_point": 9.90029697418213 }, "id": "OAIKOHHJFB_107", "locus": "GCA000209735_00062" }, { "type": "cds", "sequence": "contig_1", "start": 30337, "stop": 30510, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTTTAACAGTTACGTATGTTCCAAAAGTTCAAAAGAAATTGGTTATTGCTGATTTAGAAGACAACAAGAAAAAAATACAAGAAGATAACCAAAAACTTAAAGAGGCTATTAGCTTTAAGAAAAAAGAAGAAGTTGTTTCTGAACAAGAAACTTATGAAGACGGAATTTAA", "aa": "MLLTVTYVPKVQKKLVIADLEDNKKKIQEDNQKLKEAISFKKKEEVVSEQETYEDGI", "aa_hexdigest": "af3c8b88a027043851a269e600c6744a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6653.6034, "isoelectric_point": 5.384835243225097 }, "id": "OAIKOHHJFB_108", "locus": "GCA000209735_00063" }, { "type": "cds", "sequence": "contig_1", "start": 30520, "stop": 31092, "strand": "+", "frame": 1, "gene": "kduI", "product": "5-dehydro-4-deoxy-D-glucuronate isomerase", "db_xrefs": [ "EC:5.3.1.17", "GO:0008697", "GO:0045490", "KEGG:K01815", "SO:0001217", "UniRef:UniRef50_A0A449A2L5", "UniRef:UniRef90_C4XDS2" ], "nt": "ATGAGATTTAAAACAACATATGCAGTTTCAGCAAATGAAACATCAAGAATGACAACAGAAGAACTGAGAAGTAATTTCTTAATTGAAGATTTATTTGAAAGCGGAAAGCTTAATGTTCAATATCTTCACTATGACAGAATAATTGTTGGTGGTGCAACGCCTACATCAAAAGGTTTAAATTTAGATGAAGTAGCAAAAGAAATTGCTGCACCATATTTCTGTGAAAGAAGAGAAATAGGAATTATAAACATTGGCAATACTGGTTATATTGAATTTGACAATGGAATTGAAAAACTAGAAAATAAAGATGGTTTATATTTACCTGTAGGTAACAAAAAAATTATTTTTAAATCAGCTGATAGTAAAAAACCTGCAAAATTTTATTTCTTTTCAACTCAAGGCCTTAAACCACTACCAGTTAGAAAAGTAAATATTAAAGATGCAATTCCAAATAAATTAGGAACACCAGCTGAAAGTAATGTTAGAACAATTTACCAATATTTTCATCCAAAAGTATGTCAAACAAATTCACTATTAATGGGGCTTACAATTTTAGAACCTAACAATATGTGA", "aa": "MRFKTTYAVSANETSRMTTEELRSNFLIEDLFESGKLNVQYLHYDRIIVGGATPTSKGLNLDEVAKEIAAPYFCERREIGIINIGNTGYIEFDNGIEKLENKDGLYLPVGNKKIIFKSADSKKPAKFYFFSTQGLKPLPVRKVNIKDAIPNKLGTPAESNVRTIYQYFHPKVCQTNSLLMGLTILEPNNM", "aa_hexdigest": "28b78351f25c7f444add990abbaa8553", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "kduI" ], "truncated": "3-prime", "pseudogene": { "start": 30517, "stop": 31359, "observations": { "mutation": [ 31090, 31246, 31291 ], "stop": [ 31090, 31246, 31291 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDS2", "query_cov": 1.0, "subject_cov": 0.6785714285714286, "identity": 1.0, "score": 381.0, "evalue": 1.68e-132, "gene_start": 1, "gene_end": 190, "reference_start": 2, "reference_end": 191, "reference_sequence": "MMRFKTTYAVSANETSRMTTEELRSNFLIEDLFESGKLNVQYLHYDRIIVGGATPTSKGLNLDEVAKEIAAPYFCERREIGIINIGNTGYIEFDNGIEKLENKDGLYLPVGNKKIIFKSADSKKPAKFYFFSTQGLKPLPVRKVNIKDAIPNKLGTPAESNVRTIYQYFHPKVCQTNSLLMGLTILEPNNMWNTMPCHTHERRTECYLYIDVAEGQRVIHLMGKPQETKHLVVKNEGFVISAPWSIHSGVGTSNYAFIWAMGGENLDYTDMQGVKTIDLK" }, "paralog": false, "identity": 0.9857142857142858, "score": 556.0, "evalue": 5.18e-204, "description": "Internal stop codon at 31090, 31246, 31291. Nonsense mutation around 31090, 31246, 31291.", "psc": { "uniref90_id": "UniRef90_C4XDS2", "gene": "kduI", "product": "5-dehydro-4-deoxy-D-glucuronate isomerase", "ec_ids": [ "5.3.1.17" ], "uniref50_id": "UniRef50_A0A449A2L5", "kegg_orthology_id": "K01815", "go_ids": [ "GO:0008697", "GO:0045490" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDS2", "EC:5.3.1.17", "UniRef:UniRef50_A0A449A2L5", "KEGG:K01815", "GO:0008697", "GO:0045490" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449A2L5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A2L5" ], "product": "4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase" } }, "id": "OAIKOHHJFB_109", "locus": "GCA000209735_00064" }, { "type": "cds", "sequence": "contig_1", "start": 31099, "stop": 31248, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCATGTCATACACATGAAAGAAGAACAGAATGCTACTTATATATAGATGTAGCAGAAGGTCAAAGAGTAATTCACTTAATGGGCAAACCTCAAGAAACTAAACACTTAGTTGTTAAAAATGAAGGATTTGTCATTTCGGCTCCTTGA", "aa": "MPCHTHERRTECYLYIDVAEGQRVIHLMGKPQETKHLVVKNEGFVISAP", "aa_hexdigest": "e3d1cba795fd1407655ae716ebcf170c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5662.5078, "isoelectric_point": 6.891182136535646 }, "id": "OAIKOHHJFB_110", "locus": "GCA000209735_00065" }, { "type": "cds", "sequence": "contig_1", "start": 31359, "stop": 31556, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTAGAAATCTAAATAATTCATTAATCTATGAAAAAGAATTCAATGATACAAAACTATTAACTAAAGAAGAAATTAAAAAAGCTCTTGATTTAATAATCAAGCAAGTTGATCAAAATATTGATTATTTTGGCAAAGAATTTATGATTGCCAACACATTCAATAATAAATATCAAAAAATTTATTCTAGTGAATGA", "aa": "MFRNLNNSLIYEKEFNDTKLLTKEEIKKALDLIIKQVDQNIDYFGKEFMIANTFNNKYQKIYSSE", "aa_hexdigest": "4f717bc35b55e86fe7931c661e90bf37", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7844.878000000002, "isoelectric_point": 6.243842887878418 }, "id": "OAIKOHHJFB_111", "locus": "GCA000209735_00066" }, { "type": "cds", "sequence": "contig_1", "start": 31695, "stop": 31832, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGATTCTTGTATTCACCAAGTTGTGTTGCAGCATATAAATTAACTAAAAATCAAGAAGCAAAAGAGGCTGCTCTTTTAGCTGCAGATAATCTTATAAGTAGATATCAAGAAAAAGGCAAATTTATACAAGCTTGA", "aa": "MGFLYSPSCVAAYKLTKNQEAKEAALLAADNLISRYQEKGKFIQA", "aa_hexdigest": "008699fd48050ab2f7dac701666d2cec", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4981.7018, "isoelectric_point": 8.889368247985843 }, "id": "OAIKOHHJFB_112", "locus": "GCA000209735_00067" }, { "type": "cds", "sequence": "contig_1", "start": 32316, "stop": 32459, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAAATTACTCAAAAAGACTATAAAACAGATTTATACGAAAAATCAGCTCATTTAATTTTAAGAAATTTAATTAATAAATATCACAATAAAAAAATTGAACCTGGAACACCAATTTTATTGCATGGAGTTTATTCATGA", "aa": "MLKITQKDYKTDLYEKSAHLILRNLINKYHNKKIEPGTPILLHGVYS", "aa_hexdigest": "a2005db7a4beef07139611715a016d0b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5539.4529999999995, "isoelectric_point": 9.573958015441892 }, "id": "OAIKOHHJFB_113", "locus": "GCA000209735_00068" }, { "type": "cds", "sequence": "contig_1", "start": 32637, "stop": 32780, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGTAAATGGATCAAACTCAGTTATTGTTGCTAACGATGAAGCAGCAGTTTCGGAAACTCAACAACAATTAATGAAAGTTACTGTACCAGAAGGGGTTAGATGTAGCTTTTATTCAGTTGATAAATTAATAGAAATTTGA", "aa": "MQVNGSNSVIVANDEAAVSETQQQLMKVTVPEGVRCSFYSVDKLIEI", "aa_hexdigest": "fdbc5a0ad85c6edb553bf231e3ff5b5a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5128.764699999999, "isoelectric_point": 4.3588346481323255 }, "id": "OAIKOHHJFB_114", "locus": "GCA000209735_00069" }, { "type": "cds", "sequence": "contig_1", "start": 33133, "stop": 33240, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGACCTATACAGCAACACCAATAGTTTATGCTTTAGGTGTTGGAATAATTTTAGGGGATATTCAAACCGCTGTTATTGTTGGAGCTGCTGGTCAAACTGTTTGA", "aa": "MGTYTATPIVYALGVGIILGDIQTAVIVGAAGQTV", "aa_hexdigest": "850023f892d116746b7ff6b3a76fe1a3", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3434.9936999999995, "isoelectric_point": 4.0500284194946286 }, "id": "OAIKOHHJFB_115", "locus": "GCA000209735_00070" }, { "type": "cds", "sequence": "contig_1", "start": 33406, "stop": 33615, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATTATTAACAACATTATTATTCACATTAATGTCACCGATAAGTGAAATAGCCAAAAAACAAATTACAAAAGGCAAATTTAAAACTTTTAAATTCTTATCTCATGTAACTATTTTAGTCTTGATGATCATTACATTTGGATTTGGTTTATTAGTTGGTGTTTCAGCTAATGGAATAGCTAGATTCTCAAGCAGTATTCCAGATTGA", "aa": "MQLLTTLLFTLMSPISEIAKKQITKGKFKTFKFLSHVTILVLMIITFGFGLLVGVSANGIARFSSSIPD", "aa_hexdigest": "642d306918ad83035b34f00046d454a9", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7562.090899999997, "isoelectric_point": 10.294907951354979 }, "id": "OAIKOHHJFB_116", "locus": "GCA000209735_00071" }, { "type": "cds", "sequence": "contig_1", "start": 33646, "stop": 33939, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTCCTGCTTTAGGTTTTGCTTTAATATTAAAAGTTATGCTCAAAAAAGAATATATTGGTTTTGCTTTATTAGGTTACTTTTTAGCTTTGGTATTTGAAGTTATTTCAAAAATCACAGGTCAAGCATTCTCGGTATTAGCTCTTGCTCTTACTGCCATGGCTTTTGTCTTGATCATAATATCAATAAAAAAATTAGCAGGTATTAAAGAAGCAGCTGGAGTAGCTCGAAACATAAGTAACACAAGCAGTAATAATGAAAATAACAAGGGAGGTCAACAAGATGGAATCTAA", "aa": "MLPALGFALILKVMLKKEYIGFALLGYFLALVFEVISKITGQAFSVLALALTAMAFVLIIISIKKLAGIKEAAGVARNISNTSSNNENNKGGQQDGI", "aa_hexdigest": "dfcdad617cfb3ed09a556a990b2ab440", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10287.194299999986, "isoelectric_point": 9.631657218933103 }, "id": "OAIKOHHJFB_117", "locus": "GCA000209735_00072" }, { "type": "cds", "sequence": "contig_1", "start": 34256, "stop": 34678, "strand": "+", "frame": 2, "gene": null, "product": "PTS system mannose/fructose/sorbose family IID component", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A2J7", "UniRef:UniRef90_C4XDS6" ], "nt": "ATGTCATTGATGGGACCTTTGAGTGGTATTGGGGATTCATTAACACAATATGCAATTTATCCTTTATTTTCTGTTATAGCAATTGGTTTTGCTCAAAGCGGAAATATACTAGGACCTATTATCTTTATAGTAGGAATGAACATCACATTAATAACAATCAGATTAACTTTAGGTATTTTAGGATACAAACTAGGTGAAGTTGCAATAGCTAAATTATCTAAACACATGCAAAGCATAATTAAAATTGCTAGCATGCTTGGGGTAACAATTATTTCAGCTCTTGCATTAAGATTAACAAAAGTTAAATTTACTTTACAATTTTCACAACGTGTTGATGCAGGTGCATCAGGATTTCAAGAACAAGTTATTTCAATTCAAAATATTTTAGACAACATTATGCCACTATTTGCTTCAGGAATATGA", "aa": "MSLMGPLSGIGDSLTQYAIYPLFSVIAIGFAQSGNILGPIIFIVGMNITLITIRLTLGILGYKLGEVAIAKLSKHMQSIIKIASMLGVTIISALALRLTKVKFTLQFSQRVDAGASGFQEQVISIQNILDNIMPLFASGI", "aa_hexdigest": "6bb174903953e55ef2f94aae8d55bd27", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 33956, "stop": 34792, "observations": { "mutation": [ 33992, 34232, 34676, 34715 ], "stop": [ 33992, 34232, 34676, 34715 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDS6", "query_cov": 1.0, "subject_cov": 0.4713804713804714, "identity": 1.0, "score": 255.0, "evalue": 2.11e-83, "gene_start": 1, "gene_end": 140, "reference_start": 120, "reference_end": 259, "reference_sequence": "MMKITREVNKMESNNKDFVQVNQAQTKKLSIWTYIIVALRSYFLQSGFNYANFQGLGYANIIYPALKKIYGYGTQQFKDALLNNIEFYNTNPQCIPLITSIHIQLLNNGTSWADARTIKMSLMGPLSGIGDSLTQYAIYPLFSVIAIGFAQSGNILGPIIFIVGMNITLITIRLTLGILGYKLGEVAIAKLSKHMQSIIKIASMLGVTIISALALRLTKVKFTLQFSQRVDAGASGFQEQVISIQNILDNIMPLFASGIWVIFIYFMVTKYKWSPYKVIVVTILVGVTAGVFGILGV" }, "paralog": false, "identity": 0.9856115107913669, "score": 520.0, "evalue": 2.85e-190, "description": "Internal stop codon at 33992, 34232, 34676, 34715. Nonsense mutation around 33992, 34232, 34676, 34715.", "psc": { "uniref90_id": "UniRef90_C4XDS6", "product": "PTS system mannose/fructose/sorbose family IID component", "uniref50_id": "UniRef50_A0A449A2J7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDS6", "UniRef:UniRef50_A0A449A2J7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449A2J7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A2J7" ], "product": "PTS system mannose-specific EIID component" } }, "id": "OAIKOHHJFB_118", "locus": "GCA000209735_00073" }, { "type": "cds", "sequence": "contig_1", "start": 34792, "stop": 35199, "strand": "+", "frame": 1, "gene": null, "product": "PTS EIIA type-4 domain-containing protein", "db_xrefs": [ "RefSeq:WP_041594167.1", "SO:0001217", "UniParc:UPI00030B9569", "UniRef:UniRef100_C4XDS7", "UniRef:UniRef50_C4XDS7", "UniRef:UniRef90_C4XDS7" ], "nt": "ATGGAAACAAAAATTTTGGTAATAGGACATTCACAATTTCCCCGAGTTGTTGTTGAAGGTCTTAAATTTGTTAGTGGTATTGAAGAAGACATTGATTATAAATATATAGATAATGATCATTCAATAGATGAACTTAAAAATGATTTAGAAAAATATTTAGAAAAATGTGAAAATATAATCATTTTTGCGGATTTACCAGGCGGATCACCGCATCGTCTAGCGGTTGAAACTATTGAAAAAACTAAAAAGAAAAATGCAATAGTTATTGCTGGTGCAGGAATGCCTATGATTATGGATATTGCTTTTAAAGTTATACTAATAAAATTCAAATCATTTGACGAAATAATCTGTTTTATAGGTGAAAAGAAAAAAAATTTAGATAAATACATTATGATCACAGGAGCTTAA", "aa": "METKILVIGHSQFPRVVVEGLKFVSGIEEDIDYKYIDNDHSIDELKNDLEKYLEKCENIIIFADLPGGSPHRLAVETIEKTKKKNAIVIAGAGMPMIMDIAFKVILIKFKSFDEIICFIGEKKKNLDKYIMITGA", "aa_hexdigest": "c256892f9e95ecccdbe1aaaf2ec19991", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00030B9569", "ncbi_nrp_id": "WP_041594167.1", "uniref100_id": "UniRef100_C4XDS7", "db_xrefs": [ "SO:0001217", "UniParc:UPI00030B9569", "RefSeq:WP_041594167.1", "UniRef:UniRef100_C4XDS7" ] }, "ips": { "uniref100_id": "UniRef100_C4XDS7", "uniref90_id": "UniRef90_C4XDS7" }, "psc": { "uniref90_id": "UniRef90_C4XDS7", "product": "PTS EIIA type-4 domain-containing protein", "uniref50_id": "UniRef50_C4XDS7" }, "pscc": { "uniref50_id": "UniRef50_C4XDS7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDS7" ], "product": "PTS EIIA type-4 domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_119", "locus": "GCA000209735_00074" }, { "type": "cds", "sequence": "contig_1", "start": 35202, "stop": 35537, "strand": "+", "frame": 3, "gene": "yajC", "product": "Preprotein translocase subunit YajC", "db_xrefs": [ "KEGG:K03210", "RefSeq:WP_013354710.1", "SO:0001217", "UniParc:UPI0001E32E90", "UniRef:UniRef100_C4XDS8", "UniRef:UniRef50_C4XDS8", "UniRef:UniRef90_C4XDS8" ], "nt": "ATGGATTTAGCAGTTAAAATTGTTTTATTTATTTTACTAGGTTTATTTATTCTACAAATAATAGTTGTAACAGTTATTTTGCCTATTTTAAAAAAGAAAAGAGATGAAAAAATAGCAAGGGAACATAAAGAACTAGTTAAGAATATTAAAAGTGGTCATGAAGTTTTACTTCAAAATGGAATTTATGGAACATATGTAAATTCTAGTGGCGACGAAACTTTTAATATGAGAATAAATGAAGAAAATAATGTAATTATCAAAGTTAACAAAAGTGCAATACTTGGTTTCTTTGACAAGGATATTGCAAAAAAGATTGAACAAAGAAAACAAAAATAA", "aa": "MDLAVKIVLFILLGLFILQIIVVTVILPILKKKRDEKIAREHKELVKNIKSGHEVLLQNGIYGTYVNSSGDETFNMRINEENNVIIKVNKSAILGFFDKDIAKKIEQRKQK", "aa_hexdigest": "b6902950ec64bb2a671c1fd0db03b859", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32E90", "ncbi_nrp_id": "WP_013354710.1", "uniref100_id": "UniRef100_C4XDS8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32E90", "RefSeq:WP_013354710.1", "UniRef:UniRef100_C4XDS8" ] }, "ips": { "uniref100_id": "UniRef100_C4XDS8", "uniref90_id": "UniRef90_C4XDS8" }, "psc": { "uniref90_id": "UniRef90_C4XDS8", "product": "Preprotein translocase subunit YajC", "uniref50_id": "UniRef50_C4XDS8", "kegg_orthology_id": "K03210" }, "pscc": { "uniref50_id": "UniRef50_C4XDS8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDS8" ], "product": "Preprotein translocase subunit YajC" }, "genes": [ "yajC" ], "id": "OAIKOHHJFB_120", "locus": "GCA000209735_00075" }, { "type": "cds", "sequence": "contig_1", "start": 35550, "stop": 35891, "strand": "+", "frame": 3, "gene": "kduD", "product": "2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase KduD", "db_xrefs": [ "EC:1.1.1.127", "SO:0001217", "UniRef:UniRef50_A0A975S3K9", "UniRef:UniRef90_C4XDS9" ], "nt": "ATGGATTTTAAATTTAATAAAGACTATTTTTCAGTTAAGGACAAAGTTGTTATTATTACAGGTGCAAACATGGGATTAGGTTTAGCTTATGCTAAAGCTTTATCAAATTGTGGCGCCAAATTAGTTATTAGTCATTTTGACACAAATACAAAAGAAATAGAAAACTTTTGTGCTAAAAACAAAACAGAATTATTGCTTGTTCAAGGTGACTTAACTAAAGAAGCAGATAGAAATAAATTAATAGAAGAAGCTATTAAAAAATTCAAACATATTGATGCTCTAGTAAACAATGCTGGCACTATAATTCGTGAACCAATATTGGAATTTAGTGAAAAATCTTGA", "aa": "MDFKFNKDYFSVKDKVVIITGANMGLGLAYAKALSNCGAKLVISHFDTNTKEIENFCAKNKTELLLVQGDLTKEADRNKLIEEAIKKFKHIDALVNNAGTIIREPILEFSEKS", "aa_hexdigest": "fa7ef4aaa710400d80d15db49a94e0ac", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "kduD" ], "truncated": "3-prime", "pseudogene": { "start": 35550, "stop": 36194, "observations": { "mutation": [ 35889, 35925 ], "stop": [ 35889, 35925 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDS9", "query_cov": 1.0, "subject_cov": 0.4396887159533074, "identity": 1.0, "score": 224.0, "evalue": 2.75e-72, "gene_start": 1, "gene_end": 113, "reference_start": 1, "reference_end": 113, "reference_sequence": "MDFKFNKDYFSVKDKVVIITGANMGLGLAYAKALSNCGAKLVISHFDTNTKEIENFCAKNKTELLLVQGDLTKEADRNKLIEEAIKKFKHIDALVNNAGTIIREPILEFSEKSWNKVIDINMNAVWFLSQKVAQIMKKQGYGKIINIASMLSYQGGILVPAYTASKHAVAGLTKAFTTELAKYNIQVNAIAPGYVETANTQAIRDDAKRNQSILERIPAGHWAKPEEIAGAVVFLCSKASDYISGAIIPVDGGFLCR" }, "paralog": false, "identity": 0.9906542056074766, "score": 409.0, "evalue": 1.16e-147, "description": "Internal stop codon at 35889, 35925. Nonsense mutation around 35889, 35925.", "psc": { "uniref90_id": "UniRef90_C4XDS9", "gene": "kduD", "product": "2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase KduD", "ec_ids": [ "1.1.1.127" ], "uniref50_id": "UniRef50_A0A975S3K9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDS9", "EC:1.1.1.127", "UniRef:UniRef50_A0A975S3K9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A975S3K9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A975S3K9" ], "product": "2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase KduD" } }, "id": "OAIKOHHJFB_121", "locus": "GCA000209735_00076" }, { "type": "cds", "sequence": "contig_1", "start": 35955, "stop": 36215, "strand": "+", "frame": 3, "gene": null, "product": "2-keto-3-deoxygluconate oxidoreductase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A377BY30" ], "nt": "ATGAAAAAGCAAGGCTATGGAAAAATTATAAATATTGCATCAATGCTTTCATACCAAGGTGGAATATTAGTTCCAGCTTATACAGCTTCTAAACATGCAGTTGCAGGATTAACAAAAGCTTTTACAACTGAGCTTGCAAAATATAACATTCAAGTTAATGCAATTGCTCCAGGTTATGTAGAAACAGCAAATACACAAGCAATAAGAGATGACGCTAAAAGAAATCAATCAATTTTAGAAAGAATTCCTGCAGGTCATTGA", "aa": "MKKQGYGKIINIASMLSYQGGILVPAYTASKHAVAGLTKAFTTELAKYNIQVNAIAPGYVETANTQAIRDDAKRNQSILERIPAGH", "aa_hexdigest": "c17948bc9b73689f5eed682bff6a069d", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI0015F461D0", "query_cov": 0.8488372093023255, "subject_cov": 0.9733333333333334, "identity": 0.589, "score": 88.6, "evalue": 1.76e-21, "valid": false, "product": "SDR family NAD(P)-dependent oxidoreductase", "uniref50_id": "UniRef50_A0A377BY30" }, "pscc": { "uniref50_id": "UniRef50_A0A377BY30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A377BY30" ], "product": "2-keto-3-deoxygluconate oxidoreductase" }, "genes": [], "id": "OAIKOHHJFB_122", "locus": "GCA000209735_00077" }, { "type": "cds", "sequence": "contig_1", "start": 36314, "stop": 36811, "strand": "+", "frame": 2, "gene": null, "product": "2-dehydro-3-deoxyphosphogluconate aldolase", "db_xrefs": [ "EC:4.1.2.14", "EC:4.1.3.42", "KEGG:K01625", "SO:0001217", "UniRef:UniRef50_D5E5I3", "UniRef:UniRef90_C4XDT0" ], "nt": "ATGTCGTTAAGTGCTTTAGATTTAATTAAAAAAGAAAAACTAATTGCCGTAATAAGATATAACTCTTATGAAGAAGCAAAAAAAGCTATTGAAACATGCATAAAAGGTGGAATTAAAATAATAGAAATTACTTTAACCACACCTGAAGCTTTGAAGCTTATTAAAGAATTTTCTAAATCAAAAGTCTTAATTGGAGCTGGGACAGTTGTAAGTCATGAAGATGCCTATAAATCAGTAAAAAGTGGAGCTAAATTTTTAGTTAGTCCACACTTTAGTGATTCAGTCAACGAAGTTTCGCAAATATCAGAGATACCTTATTTAGCTGGTGTTATGACGCCAACAGAAGCAATAAGTGCTTATAGACAAGGTAGTAAATTACTAAAAGTTTTTCCAAGTGAAATTGTTGGTCCAAATTTTATAAGCGGTCTACTTAAACCTTTTCCATTTTTGGAATTGATGCCAAGTGGAGGAGTAAATTTGGATAATGCAGCAGCTTGA", "aa": "MSLSALDLIKKEKLIAVIRYNSYEEAKKAIETCIKGGIKIIEITLTTPEALKLIKEFSKSKVLIGAGTVVSHEDAYKSVKSGAKFLVSPHFSDSVNEVSQISEIPYLAGVMTPTEAISAYRQGSKLLKVFPSEIVGPNFISGLLKPFPFLELMPSGGVNLDNAAA", "aa_hexdigest": "1b6e50eb9980b0490f624c012d0f7917", "start_type": "ATG", "rbs_motif": "GGxGG", "psc": { "uniref90_id": "UniRef90_C4XDT0", "query_cov": 1.0, "subject_cov": 0.8168316831683168, "identity": 1.0, "score": 316.0, "evalue": 1.55e-108, "valid": true, "product": "2-dehydro-3-deoxyphosphogluconate aldolase", "ec_ids": [ "4.1.2.14", "4.1.3.42" ], "uniref50_id": "UniRef50_D5E5I3", "kegg_orthology_id": "K01625" }, "pscc": { "uniref50_id": "UniRef50_D5E5I3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E5I3" ], "product": "Khg/kdpg aldolase" }, "genes": [], "id": "OAIKOHHJFB_123", "locus": "GCA000209735_00078" }, { "type": "cds", "sequence": "contig_1", "start": 36915, "stop": 37427, "strand": "+", "frame": 3, "gene": "pfkB", "product": "Carbohydrate kinase PfkB domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDT1", "UniRef:UniRef90_C4XDT1" ], "nt": "ATGGCTAAAATATTATGTATCGGCGAAACTTTAATAAGACATCAAAAAGATTATTACAACAGTCAAAGCATAATTAAATATTATGTTGGCGGAGACGCTTTAAATGTAGCAACTAGTTTATCAATATTAGGCAATGATGTTGAATATTTAAGTGTTTTAAATAAAGAAAGTTTTTATTATCAAGACATTTTAAACCACTTGAATAAATACAATATTAAACAAAATTTTATGATTTATGAAAATCAAAGAATTGGTCTCTATAATGTTATCAAAGGCAATGAATTTATTAGCAGCAAAGTAATTTATGATCGTGATAATTCATGCTATAAATTAATCAAAGATTTTCAAAATTTTAATGACAAATTGCTAGCGAATATTGATTATATTTATCTCAGCGGAATAACAATTTCGATTAATAAAAAATTAAATTCTTATATTTTAGAAATATTAAAAAAAGCTAAAAAATTAAACAAAAAAATTGTTATAGATATCAATTATCGACCAACTTTATGA", "aa": "MAKILCIGETLIRHQKDYYNSQSIIKYYVGGDALNVATSLSILGNDVEYLSVLNKESFYYQDILNHLNKYNIKQNFMIYENQRIGLYNVIKGNEFISSKVIYDRDNSCYKLIKDFQNFNDKLLANIDYIYLSGITISINKKLNSYILEILKKAKKLNKKIVIDINYRPTL", "aa_hexdigest": "8f957613d390c60272f158a2246db4d6", "start_type": "ATG", "rbs_motif": null, "genes": [ "pfkB" ], "truncated": "3-prime", "pseudogene": { "start": 36915, "stop": 37730, "observations": { "mutation": [ 37425, 37497 ], "stop": [ 37425, 37497 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDT1", "query_cov": 1.0, "subject_cov": 0.5167173252279635, "identity": 1.0, "score": 332.0, "evalue": 8.45e-113, "gene_start": 1, "gene_end": 170, "reference_start": 1, "reference_end": 170, "reference_sequence": "MAKILCIGETLIRHQKDYYNSQSIIKYYVGGDALNVATSLSILGNDVEYLSVLNKESFYYQDILNHLNKYNIKQNFMIYENQRIGLYNVIKGNEFISSKVIYDRDNSCYKLIKDFQNFNDKLLANIDYIYLSGITISINKKLNSYILEILKKAKKLNKKIVIDINYRPTLWNNKYADFKKEIDPFLNLSDIVFGWISEQNYCKSNSSISAKDFDKLTCLIDKKYKNIELIISPNKVISQQIANIKSLAISNKKIYESESIKYQPIYPIGSGDSFVAAFLNYYVNNKKIKECLDFATKAYALKNYFEYDFNPCNQEQINNVSNESFEIER" }, "paralog": false, "identity": 0.992619926199262, "score": 531.0, "evalue": 1.14e-193, "description": "Internal stop codon at 37425, 37497. Nonsense mutation around 37425, 37497.", "psc": { "uniref90_id": "UniRef90_C4XDT1", "product": "Carbohydrate kinase PfkB domain-containing protein", "uniref50_id": "UniRef50_C4XDT1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDT1", "UniRef:UniRef50_C4XDT1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDT1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDT1" ], "product": "Carbohydrate kinase PfkB domain-containing protein" } }, "id": "OAIKOHHJFB_124", "locus": "GCA000209735_00079" }, { "type": "cds", "sequence": "contig_1", "start": 37599, "stop": 37904, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00294.29" ], "nt": "TTGATAATTTCACCAAACAAGGTTATTAGCCAACAAATTGCTAATATAAAATCCTTAGCTATTTCTAATAAAAAAATATATGAATCTGAATCAATAAAATATCAACCAATTTATCCAATTGGGTCGGGCGATTCCTTTGTGGCAGCTTTTCTAAATTATTATGTCAACAATAAAAAAATAAAAGAATGTTTAGATTTTGCAACTAAAGCATATGCATTAAAAAATTATTTTGAATATGACTTTAATCCTTGCAATCAAGAACAAATAAATAATGTAAGTAATGAATCTTTTGAACTTGAAAGGTAA", "aa": "MIISPNKVISQQIANIKSLAISNKKIYESESIKYQPIYPIGSGDSFVAAFLNYYVNNKKIKECLDFATKAYALKNYFEYDFNPCNQEQINNVSNESFELER", "aa_hexdigest": "219ac7e742b71f3a532614327ce8f539", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00294.29", "name": "PfkB", "length": 51, "aa_cov": 0.49504950495049505, "hmm_cov": 1.0, "evalue": 9.362342552157961e-09, "score": 31.065217971801758, "start": 21, "stop": 70 } ], "seq_stats": { "molecular_weight": 11662.07439999999, "isoelectric_point": 6.231167793273925 }, "id": "OAIKOHHJFB_125", "locus": "GCA000209735_00080" }, { "type": "cds", "sequence": "contig_1", "start": 37907, "stop": 38917, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAAAGAAAATAATATTGCCATTATCAATGAGTGCAGCAATAATGCCTGCACTAAGTTCTTCATGCACTAATTTAAAATCTGAAGGCTTTGATAAATATGGAAATAGAATACCTAGCGAAATTAATCAATTAAAAAACTATTTAATTTTTAAAGAAAGAAAAGTTGCTATTGAAAACACAAAAGCAAGTGTTGCAGCCAATGCAATTAACAATGTTGAAAATGAAGATAGATATAATCAAATACTTTCTTTCTTAGAACCTGAATTTGAAATATTACCTAAAAATTTTGAGTATCAATATAGTGCAAAAGTAATCAATAAATCAGAAGGCGATTTATTAGAAATTAATATTTTAGTAATTCAAACAAGCACAGGCTCAAAAGAAAATATTAAATTAACTTTAGGTCAAGATACTAAATTTAAAAAAGATTCTAACTTATCAAAAAGTGATTATGAGCAAATATTAAAAAACATTTTAATTGTTAATGATCTTAAATATTTAGTCGAAGAAGATGAATCAAAACTTAGCGATAATGATAAAAAGAATAAAAATAATGCAGTTAGATTTACCGATATTATCTTAAACAAATATAATGAAAATCTTTTTATGGATCATCCTCTTGCTAACTTATATTATGCTGGGACATATAATAATGGAACAGGAATAAAAGCTACTGACTCAAACGAAGTAAAAGCTAAAAAAATTTTTGATCGAGAATTAAAAGCAATAGAAGAGGCTAAAGAATTAATTTATAGAAAAGAAAGTCAACCTGTTCAAGATCCTGAAACAAAAAGAATAGTAGATGAAGGCTATAGTGATGCTCAAAAAGTTAGAGTAGCAATTCAAAAATTACTATTTTTAGCTGCTGCGTATCAAATAAAACCAAAAGGAATATTTACTAATAAGTTTTATCAAAATGCAAAATTAAAAAATATTATTGAGAATGTTTTAGATGATTTTGCAAAAAATTATTATTACAGCGGACAACAACAATATGTTAACTGA", "aa": "MKLKKIILPLSMSAAIMPALSSSCTNLKSEGFDKYGNRIPSEINQLKNYLIFKERKVAIENTKASVAANAINNVENEDRYNQILSFLEPEFEILPKNFEYQYSAKVINKSEGDLLEINILVIQTSTGSKENIKLTLGQDTKFKKDSNLSKSDYEQILKNILIVNDLKYLVEEDESKLSDNDKKNKNNAVRFTDIILNKYNENLFMDHPLANLYYAGTYNNGTGIKATDSNEVKAKKIFDRELKAIEEAKELIYRKESQPVQDPETKRIVDEGYSDAQKVRVAIQKLLFLAAAYQIKPKGIFTNKFYQNAKLKNIIENVLDDFAKNYYYSGQQQYVN", "aa_hexdigest": "5222acf1ecc6d19b1106a586c0bd355d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 38543.423000000024, "isoelectric_point": 8.490115547180178 }, "id": "OAIKOHHJFB_126", "locus": "GCA000209735_00081" }, { "type": "cds", "sequence": "contig_1", "start": 39029, "stop": 39727, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08124.17" ], "nt": "TTGCCAAATCCTCGCTATGGTGGGGCAGCACCAACAGCTATTCTAGCTTATAAACCAGTTACTAAAAAAAGAGCTCAAACAGGGGCTAATGCTGTAGATAATGCTAAGCCAATTTTAATTTCAGGTATTCTAAATGAAGACACTTCAAAAATTGAAGATTGTATTCTTTCTTTATATGACAATATCTTTCGAGATTTTGTTAAAAAAGGTGACGGTTTTTATGTTGATGGATCATATATGCAACATACAAACATACCTTATGTAGGAAGTTATGGTGAAGTTTTATTCACTAATATTGCAGATATATTCAAATATTTTGAAAATACAGATTTGGCTTTAGGCAAAGATAAAAGATTTGAAAAAATTTATCAATTTATTGAACTCTCGATTATGCCTTTTATGTTTAAAGGCGCAATAAGTGATGGTCTTAGTGGTAGAAGTATTGTTCGTGATGATCACTCAGATCTCAAAAAAGGTTTAAACATTTTAGGCTCTTTAGCAATTATTGAAAAATCTGCTTCTCCTATATATAAAAGTAGATTAAGAGAATTTATTAAAAATCAAATATCTCATTTAAATAAAACTCAACTACAAAAATTAGCATCAGAACACAAAGTTAGACCATTATTAATAGATGCTTTATTAGAAATTAAAGACTTACCAGATTTACCACATCCTCCTAGAAATAAGGAAGATTGA", "aa": "MPNPRYGGAAPTAILAYKPVTKKRAQTGANAVDNAKPILISGILNEDTSKIEDCILSLYDNIFRDFVKKGDGFYVDGSYMQHTNIPYVGSYGEVLFTNIADIFKYFENTDLALGKDKRFEKIYQFIELSIMPFMFKGAISDGLSGRSIVRDDHSDLKKGLNILGSLAIIEKSASPIYKSRLREFIKNQISHLNKTQLQKLASEHKVRPLLIDALLEIKDLPDLPHPPRNKED", "aa_hexdigest": "62471aaada51bafc485f8b5451088f2f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08124.17", "name": "Lyase_8_N", "length": 169, "aa_cov": 0.728448275862069, "hmm_cov": 0.9822485207100592, "evalue": 1.6809660828129946e-40, "score": 135.17376708984375, "start": 18, "stop": 186 } ], "seq_stats": { "molecular_weight": 26091.72250000003, "isoelectric_point": 8.74012393951416 }, "id": "OAIKOHHJFB_127", "locus": "GCA000209735_00082" }, { "type": "cds", "sequence": "contig_1", "start": 39896, "stop": 40039, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCAATATTAATCTTCATGGAAGAATTAGTGGTTTTCCTGAAGCCTCTGTTCAAGAAAATATGGATTCTTATTATCAATATGATGGCTCAACTTTTTTAGCAACTAATAATAACTATTCGCCTTATTCGAACAAATATTGA", "aa": "MFNINLHGRISGFPEASVQENMDSYYQYDGSTFLATNNNYSPYSNKY", "aa_hexdigest": "4289bd61e48713dd358c909d4c653a7a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5449.817700000001, "isoelectric_point": 4.74920482635498 }, "id": "OAIKOHHJFB_128", "locus": "GCA000209735_00083" }, { "type": "cds", "sequence": "contig_1", "start": 40364, "stop": 40588, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTTTTGTTAAGGCAGAAGTAATTAACTACAACAATACCTTAATTACTGATAAAGTTTATTTTATGGTTAATGGAATAATAATAGTTATTGGAAACACAAATATTAAAAATAAGCTAGTTAATACAACGATTGAAAATAGAATGATCATTGATAAAAACACTGATATGTTTAGTAACCAAAAAGTAAACATAAATGGAAAGGAACAAGATTTATTTATTTGA", "aa": "MGFVKAEVINYNNTLITDKVYFMVNGIIIVIGNTNIKNKLVNTTIENRMIIDKNTDMFSNQKVNINGKEQDLFI", "aa_hexdigest": "2919218f8863a3f368b4fd31959b0426", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8482.781299999999, "isoelectric_point": 8.007634162902832 }, "id": "OAIKOHHJFB_129", "locus": "GCA000209735_00084" }, { "type": "cds", "sequence": "contig_1", "start": 40655, "stop": 41095, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCATGAAGCTAAATATCCTATTATTACAAATATGAATAGTAAAAAAATGGTTCCAGTTGTTCGTGATTTTGCTTCTTTATCAATTAATCATGAACAAAATAATACTTTCATGTATGCAATTGTTCCAAACTATGAAGACAGTAAAAAATCAAATTATTTAGATATTTTAAACAACATCAAAATTATCAAGAATGATCAAAATTTCATAGCTTTAAAATATCAAAATAAAGATTCCTTAGGCCAAATTAATGATTATTATTTTGTTGCTAGTTTATATGAAAATGGAAACATTAAATATAAAGAACAACTAAACTCATTAGGAAGTTATGATAATGAAGTTTCAATAAATTACAATGAAATTCCTGAATTTGAAAGAAACTATAAAGCTTATAAAAATGGTCTTTACCTTGAAGATTTAGATCTAAAAATTAATTTATGA", "aa": "MHEAKYPIITNMNSKKMVPVVRDFASLSINHEQNNTFMYAIVPNYEDSKKSNYLDILNNIKIIKNDQNFIALKYQNKDSLGQINDYYFVASLYENGNIKYKEQLNSLGSYDNEVSINYNEIPEFERNYKAYKNGLYLEDLDLKINL", "aa_hexdigest": "20624892c748cbec32c183ed6ffe1637", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17188.1405, "isoelectric_point": 5.3910306930542 }, "id": "OAIKOHHJFB_130", "locus": "GCA000209735_00085" }, { "type": "cds", "sequence": "contig_1", "start": 41108, "stop": 41362, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATTAAGAAAGTTCAAAATTCTTCAATTTTAGATGTTTTAGTTTCAAATGATTTAAATGAAAATCAATATGAAATTGAATTTTCAAAAGCATTCCAAAATATTACTTCACGTAAAATAACAAACAAAAAATTTAGTGCAAAAAATGTTATCAATTTAAGCGAAGTAACTTTAAATAATGTTTTAGGTCAATCAAAAATTAGAGTTGATAATCGATTAGGAGAAGTTAATGATTATAATCATATTTCATGA", "aa": "MIIKKVQNSSILDVLVSNDLNENQYEIEFSKAFQNITSRKITNKKFSAKNVINLSEVTLNNVLGQSKIRVDNRLGEVNDYNHIS", "aa_hexdigest": "c46baadd3b26ba60394deb0c52212957", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9596.759499999996, "isoelectric_point": 9.048927497863769 }, "id": "OAIKOHHJFB_131", "locus": "GCA000209735_00086" }, { "type": "cds", "sequence": "contig_1", "start": 41391, "stop": 41501, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACATAAAATTATATTTGCTTTAACTCCATTCACTCTCATGCCTTTAACTGCAATAAGTTGTAGTATTGAGAGTTCAAATGATTATGAAAACGCTAATAAGTGA", "aa": "MKKHKIIFALTPFTLMPLTAISCSIESSNDYENANK", "aa_hexdigest": "37792286e1ab56c56d51f2a437f292f6", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4057.710900000001, "isoelectric_point": 7.922987174987794 }, "id": "OAIKOHHJFB_132", "locus": "GCA000209735_00087" }, { "type": "cds", "sequence": "contig_1", "start": 41526, "stop": 41762, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAATCTGAAAACATATTAATCAATAAATTAATTAAAAGTGAATTTAAGATTACTAATTTTTATAATGAAATGATTCTTAATAAAGAATTTAATATTAATGAAGTTGGTTCAAAAATAACTTACAATAAAAATTTTAAAGCATTTAATAATTTAGATGAAACAACTACAAATTTTGACCAAAATTCTTTTAAAACAGGCAAAATAGTCAATTTAAAAAAATATTTTACTTGA", "aa": "MKESENILINKLIKSEFKITNFYNEMILNKEFNINEVGSKITYNKNFKAFNNLDETTTNFDQNSFKTGKIVNLKKYFT", "aa_hexdigest": "05ac174fc673d49731b133c65794c07c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9255.451099999995, "isoelectric_point": 9.188759422302244 }, "id": "OAIKOHHJFB_133", "locus": "GCA000209735_00088" }, { "type": "cds", "sequence": "contig_1", "start": 41805, "stop": 42014, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAATCTTACTTCAATCCATTAAGTTCAAAATTTGCAGATTATGTTACAGAAGATACATTGTATTTAAAACTAACGCTATTCAATTCTTGTGCTTTGAATCTTAAAACTCAACACATAATTTATGCTTTTACAAACTCAAACAAAACAGGTAGATTAATTACTAAAGATAATACTTTAGAACTTCAAAAACTAACACAAATAAGTTAG", "aa": "MQSYFNPLSSKFADYVTEDTLYLKLTLFNSCALNLKTQHIIYAFTNSNKTGRLITKDNTLELQKLTQIS", "aa_hexdigest": "6bf9ac25f9c3518010822bd5738a8748", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7953.0176999999985, "isoelectric_point": 8.819097709655765 }, "id": "OAIKOHHJFB_134", "locus": "GCA000209735_00089" }, { "type": "cds", "sequence": "contig_1", "start": 42029, "stop": 42391, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04200.17" ], "nt": "ATGAAAAAAATTAAAATGATTTTAACGCTTAGTTCTCTTACAACTTGTTTACCAATAATTGCTACTAGTTGTAAAAATGAAAAAGAAGAAAAAATTACAAATTTTGATGAAGTTAAATCAAAATTAACTTTTGATGTTGATAATAAATCAAATATTAAAGCATCAGAATTTAAAGATACAAATCTTATTAAAATTAATAACTTACCATCTGATTTTAAAAAAGAAATTACTTTGGAAAATTATGAAGATGGAAAAATTAAATTTAAATTAATATTAATTGACAAAAATAATAAAAAATCTCAAGCATATACTATTACTATTTCAGGTTTTAAAGCAGAAACTGCTACACCTACAATTAATTGA", "aa": "MKKIKMILTLSSLTTCLPIIATSCKNEKEEKITNFDEVKSKLTFDVDNKSNIKASEFKDTNLIKINNLPSDFKKEITLENYEDGKIKFKLILIDKNNKKSQAYTITISGFKAETATPTIN", "aa_hexdigest": "88211fb482ff6b73c1ed9741f28ed660", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04200.17", "name": "Lipoprotein_17", "length": 85, "aa_cov": 0.6416666666666667, "hmm_cov": 0.9647058823529412, "evalue": 2.3227507219654155e-08, "score": 30.36296272277832, "start": 35, "stop": 111 } ], "seq_stats": { "molecular_weight": 13624.682299999977, "isoelectric_point": 9.148466682434083 }, "id": "OAIKOHHJFB_135", "locus": "GCA000209735_00090" }, { "type": "cds", "sequence": "contig_1", "start": 42563, "stop": 42841, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAAGACACTTCAAATTCACCAGTATATACTTTTGAAATATCTAATTTTGAATTTACAGAAGAATTTGACTTCGTTAATTTTGATGAAGTTGCAAGCAAATATAAAGAACTAAAATTACTTACTGCAAACAAAGCAGAAGCTAAAAATGTTATAGCTGCATACAATGCAACAAAATCATCAAGTCTTGATGCAAAAATCAAAGATAATATTTTAGTTTTAATGGCTGGTATTTTTGCTAAAGGCAAAAACTCAAGTAAGCCTCAAGCAAGTTGA", "aa": "MTKDTSNSPVYTFEISNFEFTEEFDFVNFDEVASKYKELKLLTANKAEAKNVIAAYNATKSSSLDAKIKDNILVLMAGIFAKGKNSSKPQAS", "aa_hexdigest": "43177eb6ec6e342c5437a931d5148af9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10166.370999999994, "isoelectric_point": 6.232304573059082 }, "id": "OAIKOHHJFB_136", "locus": "GCA000209735_00091" }, { "type": "cds", "sequence": "contig_1", "start": 43076, "stop": 43267, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGAATTAATTGCTAGAACTTTTGCCACTTTAAGTGATACACAAAAAACTGCAAATAAAACTAAATTAGACATTCTTTATAATGCAACAAAAATATTCTTACCAAAAGCTTCAAACTTAAATTCATTTAAAGGTGAAGATGCAAACTTGTCAGATGGCAAAAATTACAAACCTACATTTAACCGTTGA", "aa": "MLELIARTFATLSDTQKTANKTKLDILYNATKIFLPKASNLNSFKGEDANLSDGKNYKPTFNR", "aa_hexdigest": "1b942679f5eb2234a6ad148529c40b56", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7082.998200000002, "isoelectric_point": 9.630754661560058 }, "id": "OAIKOHHJFB_137", "locus": "GCA000209735_00092" }, { "type": "cds", "sequence": "contig_1", "start": 44291, "stop": 44953, "strand": "+", "frame": 2, "gene": null, "product": "nicotinate phosphoribosyltransferase", "db_xrefs": [ "EC:6.3.4.21", "KEGG:K00763", "SO:0001217", "UniRef:UniRef50_D5E5M9", "UniRef:UniRef90_C4XDT5" ], "nt": "ATGACAAGCAAAAAAATGGATAAATACATTGCTAGTTATTTTTACAAAACTGAAAAGATATTAGCAAAATATAATAAAAATAATGTAATTGTTTTACAATTTTTCCAACGCCGTGATAATAGCATGCTAGCTGGTATGCAAGAAGTATTAGATTTATTAGAAAAGAATACTGATACTTCTAAATACATTATTAGATATTTACCTGATGGCACAATTATTAATAATTTAGAAGTAGCTTTAGAATTAGAAGGTCATTATCAAGACTTCGGTAAATTCGAAGGAATGATTGATGGAATTTTAGCTCGTTCAAGCTCAATTGCTTCAAATGCTTATGATTGTGTCCAAGCTGCTAAAGGCAAAAATGTAATTTTTATGGGAGATCGAGCTGATCACTATTTAATGCAAGAAATAGACGGAAGAGCTGTTGCTTTAGCTGGAATTAAATCAGTTTCAACTGATGCTCAAAATATTCATAATTATGAATTAACCTTTGGATCAGTACCTCACATTTTAATTCAAAACTTTGATGGTGATACAGCTAAAGCTATGCAAGCTTATGGCGAATTATTCCCTAAAAATAAAATCATTTCTTTAGTTGACTACCACAATAATGTTATTACTCAAGCATTAGAATCATATAAAGTATTAGGCAAAAAACTTTGA", "aa": "MTSKKMDKYIASYFYKTEKILAKYNKNNVIVLQFFQRRDNSMLAGMQEVLDLLEKNTDTSKYIIRYLPDGTIINNLEVALELEGHYQDFGKFEGMIDGILARSSSIASNAYDCVQAAKGKNVIFMGDRADHYLMQEIDGRAVALAGIKSVSTDAQNIHNYELTFGSVPHILIQNFDGDTAKAMQAYGELFPKNKIISLVDYHNNVITQALESYKVLGKKL", "aa_hexdigest": "e0c4b595eac16f195cfadef4cc951529", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 44273, "stop": 45256, "observations": { "mutation": [ 44951 ], "stop": [ 44951 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDT5", "query_cov": 1.0, "subject_cov": 0.6358381502890174, "identity": 1.0, "score": 435.0, "evalue": 2.8e-152, "gene_start": 1, "gene_end": 220, "reference_start": 7, "reference_end": 226, "reference_sequence": "MIRMVQMTSKKMDKYIASYFYKTEKILAKYNKNNVIVLQFFQRRDNSMLAGMQEVLDLLEKNTDTSKYIIRYLPDGTIINNLEVALELEGHYQDFGKFEGMIDGILARSSSIASNAYDCVQAAKGKNVIFMGDRADHYLMQEIDGRAVALAGIKSVSTDAQNIHNYELTFGSVPHILIQNFDGDTAKAMQAYGELFPKNKIISLVDYHNNVITQALESYKVLGKKLWGVRIDTSKNMVDHMFDNEPENPEHYGVNIEQVKNLRKALDQAGAKDVKIVVSSGFNAEKIRKFEAANTPVDSYGVGQSIFKLVSSFSADAVLLNGKKQAKEGRVYRPNPKLLVYKSKRK" }, "paralog": false, "identity": 0.9938837920489296, "score": 639.0, "evalue": 4.96e-235, "description": "Internal stop codon at 44951. Nonsense mutation around 44951.", "psc": { "uniref90_id": "UniRef90_C4XDT5", "product": "nicotinate phosphoribosyltransferase", "ec_ids": [ "6.3.4.21" ], "uniref50_id": "UniRef50_D5E5M9", "kegg_orthology_id": "K00763", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDT5", "EC:6.3.4.21", "UniRef:UniRef50_D5E5M9", "KEGG:K00763" ] }, "pscc": { "uniref50_id": "UniRef50_D5E5M9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E5M9" ], "product": "nicotinate phosphoribosyltransferase" } }, "id": "OAIKOHHJFB_138", "locus": "GCA000209735_00093" }, { "type": "cds", "sequence": "contig_1", "start": 44981, "stop": 45313, "strand": "+", "frame": 2, "gene": null, "product": "Nicotinate phosphoribosyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LBM3", "UniRef:UniRef90_A0A3P8LBM3" ], "nt": "ATGGTTGACCATATGTTTGATAATGAACCTGAAAATCCAGAACATTATGGTGTAAATATTGAACAAGTTAAAAACTTGAGAAAGGCATTAGATCAAGCTGGTGCTAAGGATGTTAAAATTGTCGTAAGTTCAGGATTTAATGCTGAAAAAATTAGAAAATTTGAAGCAGCAAATACACCGGTAGATAGTTATGGTGTAGGTCAAAGTATCTTTAAATTAGTTTCAAGTTTTAGTGCTGATGCTGTACTTTTAAATGGTAAAAAACAAGCTAAAGAAGGACGTGTTTATAGACCTAATCCAAAACTTTTAGTTTACAAATCAAAAAGAAAATAA", "aa": "MVDHMFDNEPENPEHYGVNIEQVKNLRKALDQAGAKDVKIVVSSGFNAEKIRKFEAANTPVDSYGVGQSIFKLVSSFSADAVLLNGKKQAKEGRVYRPNPKLLVYKSKRK", "aa_hexdigest": "a57741ad51398ee1c19539f8136b4dc4", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 44885, "stop": 45369, "observations": { "insertion": [ 45245, 45315 ], "mutation": [ 44951 ], "stop": [ 44951, 45288, 45322 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8LBM3", "query_cov": 0.8272727272727273, "subject_cov": 0.52, "identity": 0.835, "score": 150.0, "evalue": 2.78e-44, "gene_start": 1, "gene_end": 91, "reference_start": 33, "reference_end": 123, "reference_sequence": "MDYHNNVIEQALESYKVLGNKLWGVRIDTSKNMVDHMFDNQEENPEHYGVNIEQVKNLRSALDKVGANDVKIVVSSGFNAAKIKKFEEADTPVDSYGVGAGIFKFVSSFSADAVLLNGKKKQKRVANIDLIQNCKFTILKNNQFSKTPTKQSFWKIFIINNTLQKISKFKKLSII" }, "paralog": false, "identity": 0.75625, "score": 225.0, "evalue": 2.31e-76, "description": "Internal stop codon at 44951, 45288, 45322. Frameshift due to insertion around 45245, 45315. Nonsense mutation around 44951.", "psc": { "uniref90_id": "UniRef90_A0A3P8LBM3", "product": "Nicotinate phosphoribosyltransferase", "uniref50_id": "UniRef50_A0A3P8LBM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8LBM3", "UniRef:UniRef50_A0A3P8LBM3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LBM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LBM3" ], "product": "Nicotinate phosphoribosyltransferase" } }, "id": "OAIKOHHJFB_139", "locus": "GCA000209735_00094" }, { "type": "cds", "sequence": "contig_1", "start": 45442, "stop": 45801, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDT6", "UniRef:UniRef90_C4XDT6" ], "nt": "ATGAAAAAACAATCTACAGCTTTTGTTGCCGTTGCTTTATTGCAAACATCAATTATTATCATTCTATTTATTTTAGGAATGATTGAAGCAATTAATATAAATGGAGCTTCGCTTAGAATAGGCATCTATGGTGCAGTTGGATTTACTTTAGTTACACAAATAGTATTATTATTCTTTGCATTTGTTTACAATAAACCAGGATACAATGGTAAATTGGGAATATTATTAATTGTTTTCTTATTCTTGCTTTTAGCTGCATCAATTGTTTCATTATCATACACAATTTGTTCAACAGAAGGAGCTAACATTAATAATGATGGCTATAAAGTGTTTGGTATTATTTCAACAATATTCACTTGA", "aa": "MKKQSTAFVAVALLQTSIIIILFILGMIEAININGASLRIGIYGAVGFTLVTQIVLLFFAFVYNKPGYNGKLGILLIVFLFLLLAASIVSLSYTICSTEGANINNDGYKVFGIISTIFT", "aa_hexdigest": "158bb4985583269e648457ca26a8d0c8", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A3P8LIM6", "query_cov": 1.0, "subject_cov": 0.875, "identity": 0.6809999999999999, "score": 147.0, "evalue": 1.98e-43, "valid": false, "uniref50_id": "UniRef50_C4XDT6" }, "pscc": { "uniref50_id": "UniRef50_C4XDT6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDT6" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 45379, "stop": 45858, "observations": { "mutation": [ 45799 ], "stop": [ 45799 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDT6", "query_cov": 1.0, "subject_cov": 0.7484276729559748, "identity": 1.0, "score": 216.0, "evalue": 1.62e-70, "gene_start": 1, "gene_end": 119, "reference_start": 22, "reference_end": 140, "reference_sequence": "MLNNLIIKYNLNIIFLRRELVMKKQSTAFVAVALLQTSIIIILFILGMIEAININGASLRIGIYGAVGFTLVTQIVLLFFAFVYNKPGYNGKLGILLIVFLFLLLAASIVSLSYTICSTEGANINNDGYKVFGIISTIFTWVLATIFLICTIVYAVRSK" }, "paralog": false, "identity": 0.9937106918238994, "score": 298.0, "evalue": 5.72e-105, "description": "Internal stop codon at 45799. Nonsense mutation around 45799.", "psc": { "uniref90_id": "UniRef90_C4XDT6", "uniref50_id": "UniRef50_C4XDT6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDT6", "UniRef:UniRef50_C4XDT6" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDT6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDT6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 12818.22569999998, "isoelectric_point": 9.20713291168213 }, "id": "OAIKOHHJFB_140", "locus": "GCA000209735_00095" }, { "type": "cds", "sequence": "contig_1", "start": 45968, "stop": 46267, "strand": "+", "frame": 2, "gene": "efp", "product": "elongation factor P", "db_xrefs": [ "GO:0003746", "GO:0005737", "GO:0006414", "KEGG:K02356", "SO:0001217", "UniRef:UniRef50_Q98Q55", "UniRef:UniRef90_A0A3P8LBD3" ], "nt": "ATGATTAATGTTAACACATTCAAGCCAGGAATTACATTTGAAGATGAAGGCGAAATTTTTGTTGTATTAGAAGCTCAACACTCAAAACAAGGTCGTGGACAAGCTAACGTTAAAGCTAAAGTCAAAAACTTAAGAACAGGTTCAATTACTATTAAATCATACACAGGTGGTGTTATGGTTTCTAAAGCTCACATTGACAAACGTCCAATGAATTACTTATATACAGATGGTAACAACATTATCTTAATGGACAATGAAACCTATGAACAAATTGAAGTACCTCTTAGTCATGTTGAATGA", "aa": "MINVNTFKPGITFEDEGEIFVVLEAQHSKQGRGQANVKAKVKNLRTGSITIKSYTGGVMVSKAHIDKRPMNYLYTDGNNIILMDNETYEQIEVPLSHVE", "aa_hexdigest": "7b2f40e187a81d911ad9a1851c7c4198", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "efp" ], "truncated": "3-prime", "pseudogene": { "start": 45890, "stop": 46528, "observations": { "mutation": [ 46265 ], "stop": [ 46265 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8LBD3", "query_cov": 1.0, "subject_cov": 0.4669811320754717, "identity": 1.0, "score": 200.0, "evalue": 9.8e-64, "gene_start": 1, "gene_end": 99, "reference_start": 27, "reference_end": 125, "reference_sequence": "MLFLRKIKLSSIILIHILKLNEKGEFMINVNTFKPGITFEDEGEIFVVLEAQHSKQGRGQANVKAKVKNLRTGSITIKSYTGGVMVSKAHIDKRPMNYLYTDGNNIILMDNETYEQIEVPLSHVEWEMNFIKEGSTVKVRMYKDEILDIEIDDTVTLEVIEAPDAVKGNTTTNPQKKVKVETGYELETPMFIKEGDLIVISTDTGKYNGKGK" }, "paralog": false, "identity": 0.9905660377358491, "score": 414.0, "evalue": 2.84e-150, "description": "Internal stop codon at 46265. Nonsense mutation around 46265.", "psc": { "uniref90_id": "UniRef90_A0A3P8LBD3", "gene": "efp", "product": "elongation factor P", "uniref50_id": "UniRef50_Q98Q55", "kegg_orthology_id": "K02356", "go_ids": [ "GO:0003746", "GO:0005737", "GO:0006414" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8LBD3", "UniRef:UniRef50_Q98Q55", "KEGG:K02356", "GO:0003746", "GO:0005737", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q55", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q55" ], "product": "Elongation factor P" } }, "id": "OAIKOHHJFB_141", "locus": "GCA000209735_00096" }, { "type": "cds", "sequence": "contig_1", "start": 46271, "stop": 46528, "strand": "+", "frame": 2, "gene": "efp", "product": "elongation factor P", "db_xrefs": [ "GO:0003746", "GO:0005737", "GO:0006414", "KEGG:K02356", "SO:0001217", "UniRef:UniRef50_Q98Q55", "UniRef:UniRef90_A0A3P8LBD3" ], "nt": "ATGAACTTCATTAAAGAAGGATCAACAGTTAAGGTGCGTATGTATAAAGATGAAATTTTGGACATTGAAATTGATGATACAGTTACTTTAGAAGTTATTGAAGCACCTGATGCAGTTAAAGGCAATACAACAACAAATCCTCAAAAGAAAGTTAAAGTTGAAACAGGTTATGAATTAGAAACACCTATGTTTATTAAAGAAGGTGATTTAATTGTTATTTCAACTGACACTGGCAAATATAATGGTAAAGGAAAATAA", "aa": "MNFIKEGSTVKVRMYKDEILDIEIDDTVTLEVIEAPDAVKGNTTTNPQKKVKVETGYELETPMFIKEGDLIVISTDTGKYNGKGK", "aa_hexdigest": "9c49cf9b22f296322c4146d5fc2768cf", "start_type": "ATG", "rbs_motif": null, "genes": [ "efp" ], "truncated": "5-prime", "pseudogene": { "start": 45971, "stop": 46528, "observations": { "mutation": [ 46265 ], "stop": [ 46265 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8LBD3", "query_cov": 1.0, "subject_cov": 0.4009433962264151, "identity": 1.0, "score": 167.0, "evalue": 4.48e-51, "gene_start": 1, "gene_end": 85, "reference_start": 128, "reference_end": 212, "reference_sequence": "MLFLRKIKLSSIILIHILKLNEKGEFMINVNTFKPGITFEDEGEIFVVLEAQHSKQGRGQANVKAKVKNLRTGSITIKSYTGGVMVSKAHIDKRPMNYLYTDGNNIILMDNETYEQIEVPLSHVEWEMNFIKEGSTVKVRMYKDEILDIEIDDTVTLEVIEAPDAVKGNTTTNPQKKVKVETGYELETPMFIKEGDLIVISTDTGKYNGKGK" }, "paralog": false, "identity": 0.9945945945945946, "score": 365.0, "evalue": 1.78e-131, "description": "Internal stop codon at 46265. Nonsense mutation around 46265.", "psc": { "uniref90_id": "UniRef90_A0A3P8LBD3", "gene": "efp", "product": "elongation factor P", "uniref50_id": "UniRef50_Q98Q55", "kegg_orthology_id": "K02356", "go_ids": [ "GO:0003746", "GO:0005737", "GO:0006414" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8LBD3", "UniRef:UniRef50_Q98Q55", "KEGG:K02356", "GO:0003746", "GO:0005737", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q55", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q55" ], "product": "Elongation factor P" } }, "id": "OAIKOHHJFB_142", "locus": "GCA000209735_00097" }, { "type": "cds", "sequence": "contig_1", "start": 46531, "stop": 46833, "strand": "+", "frame": 1, "gene": null, "product": "MMB_0454 family protein", "db_xrefs": [ "RefSeq:WP_013354736.1", "SO:0001217", "UniParc:UPI0001E32EA9", "UniRef:UniRef100_C4XDT8", "UniRef:UniRef50_C4XDT8", "UniRef:UniRef90_C4XDT8" ], "nt": "ATGAATTTAGTTACAACAAATTGTGGTTTAAATCTTTCTTATTCAATTACAGAAAACGTTTTTAGTCAATTGATTGATCAATGCTTTGAAGAAGTTAAAAATGTTAAATTAGCTAATGAAGCAAGAATTTCATTTGATAAAGAACAAACAAATGTTGCTTTTGTAATTGATATTAAAATTAAAAAAGGTAGTGGTCTCCAACAAGTTATTGAAAATTTAACTCAAGAATTAGAATCTCGCTGCTTAGGTTTATTAGATATTAAACCTCGTAGCATTAGACTATGCTTTGTTGGATATTATTAA", "aa": "MNLVTTNCGLNLSYSITENVFSQLIDQCFEEVKNVKLANEARISFDKEQTNVAFVIDIKIKKGSGLQQVIENLTQELESRCLGLLDIKPRSIRLCFVGYY", "aa_hexdigest": "d78de6b5c1f9791c3efd907a1110f8c4", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001E32EA9", "ncbi_nrp_id": "WP_013354736.1", "uniref100_id": "UniRef100_C4XDT8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EA9", "RefSeq:WP_013354736.1", "UniRef:UniRef100_C4XDT8" ] }, "ips": { "uniref100_id": "UniRef100_C4XDT8", "uniref90_id": "UniRef90_C4XDT8" }, "psc": { "uniref90_id": "UniRef90_C4XDT8", "product": "MMB_0454 family protein", "uniref50_id": "UniRef50_C4XDT8" }, "pscc": { "uniref50_id": "UniRef50_C4XDT8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDT8" ] }, "genes": [], "id": "OAIKOHHJFB_143", "locus": "GCA000209735_00098" }, { "type": "cds", "sequence": "contig_1", "start": 46938, "stop": 47432, "strand": "-", "frame": 3, "gene": "lemA", "product": "LemA family protein", "db_xrefs": [ "KEGG:K03744", "SO:0001217", "UniRef:UniRef50_C4XDT9", "UniRef:UniRef90_C4XDT9" ], "nt": "ATGAAAATAAGTAGCGATGCTTCATTAATTGATACTCAATTGGCTAAAAGAGCAGGAACAATTCTTAAGTTAGTTGAATCAGTAAGTTCACACATGAAATTCGAAAAAGATGTTATGACACAAGTTGCAGCATACCGTTCAGCAGTTGCTACAATTCGAGAACAATCAGGAAATACTTCAGCAGATGCTATGGCAGTACGTCAAAACTTTGAAAGTCAAACAGAATCATTCTTAACAAGATTTATGGCTCAATTCGAAAAATATCCAGAACTTAAGAGTGCTGAATTAGTTAAAGGTTTAAATGATGAAATCGTTGAATGTGAAAACAACATTGCTTCAGCTAGAAGAATTTACAATACTTCAGCCACAACATTTAACCAAGAAATCTTTACATATCCAAAAAGTGTTGTTGCTTCAAGTATGAGATTAACAACAATTCTATTATTCCAAGCTAAAGAAGCTGATAGACAAGATGTTAGTGTGAAATTGTATTAA", "aa": "MKISSDASLIDTQLAKRAGTILKLVESVSSHMKFEKDVMTQVAAYRSAVATIREQSGNTSADAMAVRQNFESQTESFLTRFMAQFEKYPELKSAELVKGLNDEIVECENNIASARRIYNTSATTFNQEIFTYPKSVVASSMRLTTILLFQAKEADRQDVSVKLY", "aa_hexdigest": "e8ec4ee496bed29dc3dfc5e235ceb26e", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [ "lemA" ], "truncated": "3-prime", "pseudogene": { "start": 46938, "stop": 47663, "observations": { "mutation": [ 47459, 47507 ], "stop": [ 47459, 47507 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDT9", "query_cov": 1.0, "subject_cov": 0.6804979253112033, "identity": 1.0, "score": 301.0, "evalue": 3.52e-102, "gene_start": 1, "gene_end": 164, "reference_start": 78, "reference_end": 241, "reference_sequence": "MCIEHKNNYIEGEKMANLYNRENSRAINEKGYEPRSMSTEVPVKVNFFGKFIWYISFIFLIPIFIHISWVNYFRRQQMKISSDASLIDTQLAKRAGTILKLVESVSSHMKFEKDVMTQVAAYRSAVATIREQSGNTSADAMAVRQNFESQTESFLTRFMAQFEKYPELKSAELVKGLNDEIVECENNIASARRIYNTSATTFNQEIFTYPKSVVASSMRLTTILLFQAKEADRQDVSVKLY" }, "paralog": false, "identity": 0.9875518672199171, "score": 459.0, "evalue": 7.48e-167, "description": "Internal stop codon at 47459, 47507. Nonsense mutation around 47459, 47507.", "psc": { "uniref90_id": "UniRef90_C4XDT9", "product": "LemA family protein", "uniref50_id": "UniRef50_C4XDT9", "kegg_orthology_id": "K03744", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDT9", "UniRef:UniRef50_C4XDT9", "KEGG:K03744" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDT9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDT9" ], "product": "LemA family protein" } }, "id": "OAIKOHHJFB_144", "locus": "GCA000209735_00099" }, { "type": "cds", "sequence": "contig_1", "start": 47505, "stop": 47621, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAATTTATACAATCGTGAAAACAGTCGTGCTATTAATGAAAAAGGCTATGAACCACGTTCAATGAGTACAGAAGTACCAGTTAAAGTAAACTTTTTTGGAAAATTTATTTGA", "aa": "MANLYNRENSRAINEKGYEPRSMSTEVPVKVNFFGKFI", "aa_hexdigest": "3130e5d19c8ce1b5d638747949276b32", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4438.0085, "isoelectric_point": 9.39608974456787 }, "id": "OAIKOHHJFB_145", "locus": "GCA000209735_00100" }, { "type": "cds", "sequence": "contig_1", "start": 47707, "stop": 47904, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF014FE", "PFAM:PF02911.23" ], "nt": "ATGCAAGAAGCAATCAATATTATTAGAGCTTATGAGATGAATCCTGGTGCTTACACTTTTATTGATAATAAAAGAATTAAGATCTTTTTTGCAACCAAAAAAGAAATTAAAAATGTACCTAAATTAAAATTTAAAGATGGTTATTTATTTGCCACTGATTATCAATATGAATCTAAAAAACGAGTTAAGCTGGCATAG", "aa": "MQEAINIIRAYEMNPGAYTFIDNKRIKIFFATKKEIKNVPKLKFKDGYLFATDYQYESKKRVKLA", "aa_hexdigest": "161e228b91d84bd13897f2a070d15771", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI002FF014FE", "query_cov": 0.9846153846153847, "subject_cov": 0.810126582278481, "identity": 0.531, "score": 76.6, "evalue": 4.47e-17, "valid": false, "uniref50_id": "UniRef50_UPI002FF014FE" }, "pscc": { "uniref50_id": "UniRef50_UPI002FF014FE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF014FE" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02911.23", "name": "Formyl_trans_C", "length": 33, "aa_cov": 0.5076923076923077, "hmm_cov": 1.0, "evalue": 6.240015814057673e-07, "score": 25.66942024230957, "start": 3, "stop": 35 } ], "seq_stats": { "molecular_weight": 7740.009099999999, "isoelectric_point": 9.776259803771971 }, "id": "OAIKOHHJFB_146", "locus": "GCA000209735_00101" }, { "type": "cds", "sequence": "contig_1", "start": 48016, "stop": 48543, "strand": "-", "frame": 2, "gene": "fmt", "product": "methionyl-tRNA formyltransferase", "db_xrefs": [ "EC:2.1.2.9", "GO:0004479", "GO:0005737", "GO:0071951", "SO:0001217", "UniRef:UniRef50_D3VRI0", "UniRef:UniRef90_C4XDU0" ], "nt": "ATGAAATTATTATTAGCCGGAACTCCTGCTTTTGCAGTTGAAATATTTGAAAAATTAATCAACAACTTTGAAGTTGTTGCAATTGTTTCGCAACCTGATCGCCCAAGTGTCAGAGGTCATAAATTAGCTCCAACGCCAACAAAATTATTAGCTCAAAAATATAACATTAAATGTTTTCAACCAGAAAAAATATCACAAATAAAAGATGAATTACAAACTTTAAATTATGACTATTTAATTACCTGTGCCTTTGGTCAATATATTCCTGAAAGTGTTTTAAATATTGCTAAAAAATTAAGTCTAAATATTCATGGTTCTTTACTTCCAAAATATCGTGGAGCAGCACCAATTCAATATTCACTTTTAAATAATGATCAAGAAACTGGTATTAGTCTAATGGAAATGATTAAACAAATGGATGCAGGTGATGTTTTTGTCCAAAAAGCCATTAAAATAGATGAATATGACACAGCAAGTACTTTATTTAATAAGCTTTCAAAGCTTAGTGCAGATAACATTGTGCAATGA", "aa": "MKLLLAGTPAFAVEIFEKLINNFEVVAIVSQPDRPSVRGHKLAPTPTKLLAQKYNIKCFQPEKISQIKDELQTLNYDYLITCAFGQYIPESVLNIAKKLSLNIHGSLLPKYRGAAPIQYSLLNNDQETGISLMEMIKQMDAGDVFVQKAIKIDEYDTASTLFNKLSKLSADNIVQ", "aa_hexdigest": "82b0558bc26312d350f2db7090d9a928", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FF2EE64", "query_cov": 0.9942857142857143, "subject_cov": 0.9560439560439561, "identity": 0.644, "score": 227.0, "evalue": 1.77e-73, "valid": false, "product": "methionyl-tRNA formyltransferase", "uniref50_id": "UniRef50_A0A3B0PK42" }, "genes": [ "fmt" ], "truncated": "5-prime", "pseudogene": { "start": 47713, "stop": 48543, "observations": { "mutation": [ 48018 ], "stop": [ 48018 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDU0", "query_cov": 1.0, "subject_cov": 0.6294964028776978, "identity": 1.0, "score": 342.0, "evalue": 1.43e-117, "gene_start": 1, "gene_end": 175, "reference_start": 1, "reference_end": 175, "reference_sequence": "MKLLLAGTPAFAVEIFEKLINNFEVVAIVSQPDRPSVRGHKLAPTPTKLLAQKYNIKCFQPEKISQIKDELQTLNYDYLITCAFGQYIPESVLNIAKKLSLNIHGSLLPKYRGAAPIQYSLLNNDQETGISLMEMIKQMDAGDVFVQKAIKIDEYDTASTLFNKLSKLSADNIVQWLNDLDQNKLKRIKQDESKVTLSPKLEKEKALLNESLTMQEAINIIRAYEMNPGAYTFIDNKRIKIFFATKKEIKNVPKLKFKDGYLFATDYQYESKKRVKLA" }, "paralog": false, "identity": 0.9963768115942029, "score": 537.0, "evalue": 7.79e-197, "description": "Internal stop codon at 48018. Nonsense mutation around 48018.", "psc": { "uniref90_id": "UniRef90_C4XDU0", "gene": "fmt", "product": "methionyl-tRNA formyltransferase", "ec_ids": [ "2.1.2.9" ], "uniref50_id": "UniRef50_D3VRI0", "go_ids": [ "GO:0004479", "GO:0005737", "GO:0071951" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDU0", "EC:2.1.2.9", "UniRef:UniRef50_D3VRI0", "GO:0004479", "GO:0005737", "GO:0071951" ] }, "pscc": { "uniref50_id": "UniRef50_D3VRI0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D3VRI0" ], "product": "Methionyl-tRNA formyltransferase" } }, "id": "OAIKOHHJFB_147", "locus": "GCA000209735_00102" }, { "type": "cds", "sequence": "contig_1", "start": 48785, "stop": 49006, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTAAAGGTATATTTGAACATGATAGCAATAATGTAAAAATTATTTATGTTGTTTCATTATTAGCACATATAATCATTCATATTACAATGATTGCTATTCTTGTTTATGCAATTAAAGCATTTGTAAAATCAATAGCTTCGAAAAATGTAATTAGCATTATTTTAAATATTTTGGTATTTGCGGCAGTTGTTTTAACAATTATTTGTTATGTTGGCTGA", "aa": "MIKGIFEHDSNNVKIIYVVSLLAHIIIHITMIAILVYAIKAFVKSIASKNVISIILNILVFAAVVLTIICYVG", "aa_hexdigest": "0a424a6c8e1bc6c805bd31726ec706d8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8023.816999999997, "isoelectric_point": 9.30312633514404 }, "id": "OAIKOHHJFB_148", "locus": "GCA000209735_00103" }, { "type": "cds", "sequence": "contig_1", "start": 49482, "stop": 49625, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTCTTCAATAGCGGCGTATATTTTAATAAGATTATTTGATAAAAAACGTTTAGCTGACATTAGTGATTTTCTCGGTGAACGTTCTGTTGAAATTCATGAACAAGTTGAGCAAAGCAAAAAAGATCGTAACAAAATGTGA", "aa": "MASSIAAYILIRLFDKKRLADISDFLGERSVEIHEQVEQSKKDRNKM", "aa_hexdigest": "49879b9c837c8368de215a3a0fab766b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5481.2681, "isoelectric_point": 8.120969581604005 }, "id": "OAIKOHHJFB_149", "locus": "GCA000209735_00104" }, { "type": "cds", "sequence": "contig_1", "start": 49917, "stop": 50264, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAAATATCAACCTATTCAAAATTCAGCAAGTATCGAAATTGTTCAAAATAACCACGACTACAAATTCAATAGTGTAAGTAGTCGTGTTTATCGTGATCCAAAAAATCAAATATCACCTCGTGTTTATCGCGTCATCCGTGCTGAACAAATGCTTAAGATTTTAATCCTTTCTATTTCATTTGTAATTATGGCTGCTTCAATTATTCTCTTCACATTAGTCTATGCTAAAGTTGGTATCTTCAACAAAGAACAAAGATTAGTTGGCTACCTTGTACTTTTTGGATCAAGTGCCTTTATCTCAATGGCAATTGGTTTTAAAAATCTGATTGAAAATATTCAATGA", "aa": "MRKYQPIQNSASIEIVQNNHDYKFNSVSSRVYRDPKNQISPRVYRVIRAEQMLKILILSISFVIMAASIILFTLVYAKVGIFNKEQRLVGYLVLFGSSAFISMAIGFKNLIENIQ", "aa_hexdigest": "1f470b6d0be182ce1045367f9bd195de", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13219.433999999987, "isoelectric_point": 10.056503868103025 }, "id": "OAIKOHHJFB_150", "locus": "GCA000209735_00105" }, { "type": "cds", "sequence": "contig_1", "start": 50492, "stop": 53287, "strand": "-", "frame": 1, "gene": "smc", "product": "chromosome segregation protein SMC", "db_xrefs": [ "GO:0005524", "GO:0007059", "GO:0030261", "KEGG:K03529", "SO:0001217", "UniRef:UniRef50_Q98PK8", "UniRef:UniRef90_C4XDU3" ], "nt": "ATGGATGATGTTATCTTCGCTGGTTCTAAAACTGCAAAACCTATGGATAAAGCAGTAGTTACTTTAACTTTTGATAATCGAAATGGTATTAGTTCAATCCCGCATGAAACTATTTCAATTAGTCGTGTGCTTGAAAGAGGAAATGGAAACAACCAATATTTCTTAAATGGCGAACCATGTCGTCAAAAAGATATTAAAGATATTGCTATGGAAAGCGGTATTGGTAAAAGTTCATTAGCTATTATTTCTCAAGGTACAGTTAGTGATATTGCTGAAGCTACACCTGAAGAAAGAAAGTCTATTTTTGAAGAAGCTGCTGGTGTTTCAAAATACAAATTTCGTAAAAAAGAAGCTTTAAGCAAATTGGCTAAAACTCAAGAAGGTTTAGACCAAATTAAACTTGTTATTAGTGAAATTGAAAGAAAATTAATTCCATTAAGAAAACAAGCAGAAAAAGCTAAAGTTTACATTGCTAAAAGTGAAGAATTAAAAGCTGTTGAAGTTGGTCTTTTAGTAGACAACATCAAAACTTTTGGTGATATGTACGAAAGACTTGTAACTGAACTTGAAGGTGTTCAAGAAACTAAAAATGATCTTGATGCTCGTATTTCATCAGCTGAAGCTGCTATTACTCAAAATACTAAATTCAGATCAGAAGTTGAAGCCGAATTAAAAGAACTTTCAATTGAATATGAAACAGTTAAAGAAAGATTAAACAAATTAAACATTTCTTTAGCTAAAGAAAATGAACGTGAAAGATTAATTGCTGCTGGTGAAATTCAAGCAAACCAAGCTGAACAAATCGAAGCATACAAAACTATGCTTACAAGACATGCAAATAAATTAAATTACTACGCTCGTGAAATTGAAAATCAAGCAAACCAAATTGCTGAAAACCAAAAAACAATTTCAAAATATGACGAAGAAATTAATTTAATGAACATTAAAATTAACAAAAAACGTACAGACTTATTAAAAATTGAAACACAGTTAAACTTGCTTAACCAACAAAAAGAAAGTAACTCTTTATTATTTAAAGGGACCAAAAACATTTTAGAAAATAAAGCTTACTTTGGAAAAGCACTAAAAGGAACTGTAGCTGATTTAATTAAAGTTACAACAGAATATATTCCTGCTATTGACTCAATTTTAGCTAATGCTTCACAACACTTAGTGGTTGATAAAGCTGAAACAGCAGTTAAAGCTGTTAACTTCTTAAAAGATAATAATGGTGGTCGTGCTACATTTATTCCACTCACATCAATTCAACCAAAAATGATTCGTGATGATCATCTTTTAGCAATCCAAGGACACCCTGGATTTATTGATGTTGCAAGTAATTTAGTAACAATTGATCCTCAATATGAAATTCTTAATAAATTCCTTTTAGGTAATGTTATTGTTGCTAGCGACATTGAAAATGCTAACAAAATTTCAAGTATTCTCGAACGTCGTTACATGGTAGTTTCACTTGATGGTGACACCATTCGTTCAGGCGGTGTGATTATTGGTGGAGCTAAAAATACAGCTCAATCATTACTTGGATTTGATGAAAAAATTAAAGAGCTTGAAAATATTGTTACAGGTATGAGAGCAGCAGTTCAAGCTGATGAATCAAAAATCAATGAAATAAGAAACTATCGTCGTAGTGCTCTTGAATTAGACAGCCAATTAAAACAACAAATTACTAGTCTAAAAACCCAAAAGAGCAGTGAACAAGGTCTTTACGACGAATTACAATTAAAACTTCAAAATATTACAAACAAAGAATTCAAAGTTAAAGAAGGTGTTTCAGCATCAAGCCCTGAAGCTTCAATTGAAGTTTTAGAAAGTCAAAAAATTGCTCTTGAATCACAATTCAGAGCTAAAAATGAACGTGTAAATACAATTGCTGCCGAAATCAATTCAACTTTAGCTCTTAAAAATGATTATGAAGCTACACGTCGTAAATTAACAGATGCTTTCTCAGCTAAAATTGGTGAAAGAGAAAGATCAAAAACTTTACTTGAACAAAGTAGAGAAAGACTTGCTTCTCAATACAAAATGACATTTGAAGCAGCTGAAAAAGTTCATCACTTAGAAATGAATCGTGATCAAGCTGAAGAAATTGTTCGTCAATTAAGAGAAGAAATTGAAGGCTTAGGTAACGTTAACTTAGAAGCTCTTGAACAATTAGTTGAAGAAGAAAAACGTTACAATGAATATGCCGAAGGTGAAAGAGAATTAAGTGCTGCAAAAGAAACACTTGAAAGCGCTATTGCAGACATGGATAAAATCATTATCACTCGTCTAACTAACATTATTAATGATGTTAATGATGAATTTAACAATGTCTTTAGAACAATGTTCGGTGGTGGTACTGCAAAATTATTTTTCAGCAACCCTAAAGATATTTTAGAATCAGGTGTTGAAATCGAAGCTCAACCTCCTGGAAAAAATATTAAAAACCTTAAATTATTCTCAGGCGGTGAAAAATCACTTATCGCTATCTCATTATTATTTGGTATTCTTAAAGCTCGTCCATTACCTCTTTGTATCCTTGACGAAGTTGAAGCTGCACTTGACGAAGCTAATGTTGTTCGTTATGCAGAATACCTTCAACAACTTAAAGAAAAAACTCAATTCTTGGTTATTACACACCGTCATGGAACAATGTCAAGAGTTGACACATTATTTGGTGCAACAATGCAAAACCGTGGTGTTACAAGCTTCTTCAGCATTCAATTAGCAGATGCTAAAAAACTTGTTGCAGACGAACAAAAAGAAGAATTAGAACCTTCAAACAAAAACAAATAA", "aa": "MDDVIFAGSKTAKPMDKAVVTLTFDNRNGISSIPHETISISRVLERGNGNNQYFLNGEPCRQKDIKDIAMESGIGKSSLAIISQGTVSDIAEATPEERKSIFEEAAGVSKYKFRKKEALSKLAKTQEGLDQIKLVISEIERKLIPLRKQAEKAKVYIAKSEELKAVEVGLLVDNIKTFGDMYERLVTELEGVQETKNDLDARISSAEAAITQNTKFRSEVEAELKELSIEYETVKERLNKLNISLAKENERERLIAAGEIQANQAEQIEAYKTMLTRHANKLNYYAREIENQANQIAENQKTISKYDEEINLMNIKINKKRTDLLKIETQLNLLNQQKESNSLLFKGTKNILENKAYFGKALKGTVADLIKVTTEYIPAIDSILANASQHLVVDKAETAVKAVNFLKDNNGGRATFIPLTSIQPKMIRDDHLLAIQGHPGFIDVASNLVTIDPQYEILNKFLLGNVIVASDIENANKISSILERRYMVVSLDGDTIRSGGVIIGGAKNTAQSLLGFDEKIKELENIVTGMRAAVQADESKINEIRNYRRSALELDSQLKQQITSLKTQKSSEQGLYDELQLKLQNITNKEFKVKEGVSASSPEASIEVLESQKIALESQFRAKNERVNTIAAEINSTLALKNDYEATRRKLTDAFSAKIGERERSKTLLEQSRERLASQYKMTFEAAEKVHHLEMNRDQAEEIVRQLREEIEGLGNVNLEALEQLVEEEKRYNEYAEGERELSAAKETLESAIADMDKIIITRLTNIINDVNDEFNNVFRTMFGGGTAKLFFSNPKDILESGVEIEAQPPGKNIKNLKLFSGGEKSLIAISLLFGILKARPLPLCILDEVEAALDEANVVRYAEYLQQLKEKTQFLVITHRHGTMSRVDTLFGATMQNRGVTSFFSIQLADAKKLVADEQKEELEPSNKNK", "aa_hexdigest": "20505c0f9b5a90f00739f5cc1e29eb6d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XDU3", "query_cov": 1.0, "subject_cov": 0.9300699300699301, "identity": 1.0, "score": 1701.0, "evalue": 0.0, "valid": true, "gene": "smc", "product": "chromosome segregation protein SMC", "uniref50_id": "UniRef50_Q98PK8", "kegg_orthology_id": "K03529", "go_ids": [ "GO:0005524", "GO:0007059", "GO:0030261" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PK8" ], "product": "Chromosome partition protein Smc" }, "genes": [ "smc" ], "id": "OAIKOHHJFB_151", "locus": "GCA000209735_00106" }, { "type": "cds", "sequence": "contig_1", "start": 53330, "stop": 53467, "strand": "-", "frame": 1, "gene": null, "product": "AAA family ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFD687", "UniRef:UniRef90_UPI0024CCD326" ], "nt": "ATGAAACTCATTAAAGTTGAAGCCCACGGATTCAAATCATTTGCTGATCCTATTGTGCTTAAATTTGATGGTGGAGTAGCCGGAATTGTTGGCCCTAATGGTTCTGGTAAATCAAATATTAACGATGCTATCAAATGA", "aa": "MKLIKVEAHGFKSFADPIVLKFDGGVAGIVGPNGSGKSNINDAIK", "aa_hexdigest": "566a740d3cb0dc930db18b8fd124f59e", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 53234, "stop": 53467, "observations": { "mutation": [ 53332 ], "stop": [ 53332 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0024CCD326", "query_cov": 1.0, "subject_cov": 0.5555555555555556, "identity": 0.9109999999999999, "score": 87.8, "evalue": 7.69e-22, "gene_start": 1, "gene_end": 45, "reference_start": 1, "reference_end": 45, "reference_sequence": "MKLVKLEAHGFKSFADPIVLRFDGGVAGIVGPNGSGKSNINDAIRWVLGEQSSKELRGDTMEDVIFAGSKTVQPMNKNRFL" }, "paralog": false, "identity": 0.8441558441558441, "score": 136.0, "evalue": 1.62e-43, "description": "Internal stop codon at 53332. Nonsense mutation around 53332.", "psc": { "uniref90_id": "UniRef90_UPI0024CCD326", "product": "AAA family ATPase", "uniref50_id": "UniRef50_UPI0021DFD687", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0024CCD326", "UniRef:UniRef50_UPI0021DFD687" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0021DFD687", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFD687" ], "product": "AAA family ATPase" } }, "id": "OAIKOHHJFB_152", "locus": "GCA000209735_00107" }, { "type": "cds", "sequence": "contig_1", "start": 53565, "stop": 54140, "strand": "-", "frame": 3, "gene": "rnc", "product": "ribonuclease III", "db_xrefs": [ "EC:3.1.26.3", "GO:0003723", "GO:0004525", "GO:0006364", "GO:0006396", "SO:0001217", "UniRef:UniRef50_C4XDU4", "UniRef:UniRef90_C4XDU4" ], "nt": "GTGAGTGAAAGAGTTGGCAATTATCAAGATCTTGAATTTCTCGGAGACGCTATTTTACAGTATCTTTCGTCAGATTTTGTATTCAAAAAATTTCCAAATTTAAGTGCTGGTAATATGACTTTAATTCGTTCTAAATTAGTTTGCACAAAGTCACTAAATGAAATTAGCACTAAAATTGGATTAAAAGATTTTTTACTAACTGCACCTGGTCAAATGAGTAAAGATGCTAAACGCTCAGTTAAAGTTGGCGCTGACATTTTTGAAGCCTTTATTGCTGCTATTTATTTGGATACAAATCTAAAAGTAGTCAAAGAATTTTTAAACAAAACTGTTTTTGATATCAAAATTGGAGACTTTGACATCAAGCATCTTAAAGATGCTAAAACTGCTTTTCAAGAACAAATTCAATCATTCAGTAAACTAGCTGTTATGTATATAGTTAGTGAAAAAAGTCCAACTGAATTTGAAGCTCACGCAGTTCACGACAATAAAATTTATGGTATTGGTTTTGGTAAAAGCAAAGCTGAAGCCGAAGAAAATGCTGCACTAGATGCATTGCATAAAATGAAAAAATAA", "aa": "MSERVGNYQDLEFLGDAILQYLSSDFVFKKFPNLSAGNMTLIRSKLVCTKSLNEISTKIGLKDFLLTAPGQMSKDAKRSVKVGADIFEAFIAAIYLDTNLKVVKEFLNKTVFDIKIGDFDIKHLKDAKTAFQEQIQSFSKLAVMYIVSEKSPTEFEAHAVHDNKIYGIGFGKSKAEAEENAALDALHKMKK", "aa_hexdigest": "8e9a13a2f6ad83f7d183e08768bbcbaa", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XDU4", "query_cov": 1.0, "subject_cov": 0.809322033898305, "identity": 0.995, "score": 371.0, "evalue": 2.38e-129, "valid": true, "gene": "rnc", "product": "ribonuclease III", "ec_ids": [ "3.1.26.3" ], "uniref50_id": "UniRef50_C4XDU4", "go_ids": [ "GO:0003723", "GO:0004525", "GO:0006364", "GO:0006396" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDU4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDU4" ], "product": "Ribonuclease 3" }, "genes": [ "rnc" ], "id": "OAIKOHHJFB_153", "locus": "GCA000209735_00108" }, { "type": "cds", "sequence": "contig_1", "start": 54828, "stop": 55289, "strand": "-", "frame": 3, "gene": "plsX", "product": "phosphate acyltransferase PlsX", "db_xrefs": [ "EC:2.3.1.274", "GO:0008610", "GO:0043811", "KEGG:K03621", "SO:0001217", "UniRef:UniRef50_Q98R48", "UniRef:UniRef90_C4XDU5" ], "nt": "ATGTACAAAATAGCATTTGATATTAATGGCAATGACAACGGCATTAATTCAGCTTTAGCAGCAACTAAATTATTTTTAAAAACCAACAAAAATTATGCAATTATTTTAGTTGGTCCTAAGGCTGAAATTGAAAAATATTATGGCAAAAACATGCCAGAAAATTTAGAAATAATTGACAACTCATCAGTGCCAAGCGATGTTAAAAACATTCGCCAATCTTTAAGAGAAAATACCTCAATGAATGTTGCACTTGATTTAGTAGCAAATAAAAAAGCAGATGCTGTACTTTCTTCAGGAGACTCTGGCACATATCTTGCAGCTGCTACTTTTAAAGTTAAAAGATTAGAAGGCATTTCAAGAGCAGCATTTATGCCCTTAATGCCTACAATCACAGGACGTAAATTCTTACTTCTTGATGTTGGAGCTAACTTAGAAACCACAAGTGATTATTTAGTTGAATGA", "aa": "MYKIAFDINGNDNGINSALAATKLFLKTNKNYAIILVGPKAEIEKYYGKNMPENLEIIDNSSVPSDVKNIRQSLRENTSMNVALDLVANKKADAVLSSGDSGTYLAAATFKVKRLEGISRAAFMPLMPTITGRKFLLLDVGANLETTSDYLVE", "aa_hexdigest": "3c509b4348dba4600e103eb9c6b8ce5d", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "plsX" ], "truncated": "5-prime", "pseudogene": { "start": 54525, "stop": 55346, "observations": { "mutation": [ 54830 ], "stop": [ 54830 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDU5", "query_cov": 1.0, "subject_cov": 0.43714285714285717, "identity": 1.0, "score": 292.0, "evalue": 5.22e-97, "gene_start": 1, "gene_end": 153, "reference_start": 20, "reference_end": 172, "reference_sequence": "MVLNLTTTSLLVLNKIRSKMYKIAFDINGNDNGINSALAATKLFLKTNKNYAIILVGPKAEIEKYYGKNMPENLEIIDNSSVPSDVKNIRQSLRENTSMNVALDLVANKKADAVLSSGDSGTYLAAATFKVKRLEGISRAAFMPLMPTITGRKFLLLDVGANLETTSDYLVEWTKIANSFDRVLLKNLAPRCALINIGTEDYKGTELIKEANNKLKEQHQLNYIGFQEPRDLLNGVCDVAVIDGYGGNLILKSLEGAILSFKNLLTSKIKLKPIRKLGYLLARGAFKDVAETLDYRNVGAAWIAGVNGVVIKSHGSSDEKAYYGALNQIKLALDNHILSEVKKDLNINEE" }, "paralog": false, "identity": 0.9963369963369964, "score": 525.0, "evalue": 3.44e-191, "description": "Internal stop codon at 54830. Nonsense mutation around 54830.", "psc": { "uniref90_id": "UniRef90_C4XDU5", "gene": "plsX", "product": "phosphate acyltransferase PlsX", "ec_ids": [ "2.3.1.274" ], "uniref50_id": "UniRef50_Q98R48", "kegg_orthology_id": "K03621", "go_ids": [ "GO:0008610", "GO:0043811" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDU5", "EC:2.3.1.274", "UniRef:UniRef50_Q98R48", "KEGG:K03621", "GO:0008610", "GO:0043811" ] }, "pscc": { "uniref50_id": "UniRef50_Q98R48", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98R48" ], "product": "Phosphate acyltransferase" } }, "id": "OAIKOHHJFB_154", "locus": "GCA000209735_00109" }, { "type": "cds", "sequence": "contig_1", "start": 55303, "stop": 56928, "strand": "-", "frame": 2, "gene": "dhaL", "product": "DhaL domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354744.1", "SO:0001217", "UniParc:UPI0001A8C31B", "UniRef:UniRef100_C4XDU6", "UniRef:UniRef50_A0A449B6P5", "UniRef:UniRef90_C4XDU6" ], "nt": "ATGACTAAGATTTTAGACGGTAAGCTTTTTTGTCAACTTTTTATTTCCGGAGCTAATAACATTATTAACTCTAAAAATAAGATCGACGCTTTAAATGTTTTTCCTGTTCCAGATGGCGACACAGGAACTAACATGTCATCAACAGCTCAAGCTGCTGCTGATGCTTTGGCTTTAAATAACTCAAATCACTTAGGTGAAGTAACTAACACTATTTCAAGAAATATGCTTTTAGGAGCTCGCGGAAACTCAGGTGTTATTTTAAGCCAAATTTTTAAAGGTTTTGCAATTGCGTTTAAAGAAGTTGAAAGCGTTGACATTAAAGGACTTTTAGAAGGATTTAAGCAAGCGACTAAAAAAGCTTATTCATCAGTTTTAAAACCTGTTGAAGGTACCATTTTAACAGTTATTAGAGAAACAACAGAAGCATTAGAAAAAGAAGTAAATAATAAAACTTCACTAGAAGAATTTTTCAAATTAGCTAAAAATGCAGCTCGTAAAGCATGTGACAATACTCCTAACAAATTAAAGATCTTACGTGAAGTTGGCGTAACTGACTCAGGTGGTGAAGGATTATATACAATTATTAGTGGTATGGAAGCAGCATTAAATGCTGAAGCCATTAAAATTAGTGAATCAGAAGAAAACATTGATACTTTTATCAGCTCAAAAGAAGTTTATGATGGCGAATTTGGTTATTGTACTGAATTCATTATTGAATTAAATGACGAAAAAGACTTTGATAAAGCTAATTTTGAAAAATCATTACTTAAAAAAGCTAATTCTTTAGTTGTAGTTCAAGATGAAAAAATTGTTAAAGTTCATGGTCATACATTAAAACCTGGTGATATGTTAAACTTTGGTCAAAAATATGGTGAATTTATTAAAATAAAGTCAGAAAATATGACTCGTCAAGCAAGTGAAAGTCGTAATAAAAACGTTATTATTAACCAAAATGATGAACAATCAAACAAAAAATGTGCCATTATTTCATGTAACTTAGGCAGTGGAATTATTGCAAGAATGAAAGAATTTGGAGTTGACGCTATTATTGAAAGTGGTCAGACACAAAACCCTTCAGCTCAAGATATTATTGATGCAATTAACTCAGTAAATAGCAAAAACGTTTTCATTTTACCTAATAACTCAAACATTTTCCTAGCAGCTGAACAAGCAGCTCAAGCTATTACAAATAAAAAGGTATTTATTATTCAAACTAGAAGTCAAATTCAAGGATTAACAGCTTTAATGAACTTTAATTCTGAATCAAGTCCTAAAGAAAACCGTGAAACAATGTCTGAATCTATTAAGCTTGTAACCACAGCTGAAGTAACCCAAGCAATTAGAAGCACAAAAATTGACAATGTTAAAATCAAAGAAGGTCAATTCATTGGCATTACTGATGGCAAGATTTTAGTTAGTGAAAATAACTACATTGATGCAGCTAAAAAATTGATTAAAAAATCAATTAACAGTGATCATGAACTTGTTACAGTTTACTATGGAAATGATGCAAGTGAAGTTGATGCTAACGAAATTAAAAACTTTTTAGAAAGCCATTATGATGTTGAAGTTGAAGTAGTGAATGGTGCTCAACCTAACTACCACTTCATTATTGGTTTTGAATAA", "aa": "MTKILDGKLFCQLFISGANNIINSKNKIDALNVFPVPDGDTGTNMSSTAQAAADALALNNSNHLGEVTNTISRNMLLGARGNSGVILSQIFKGFAIAFKEVESVDIKGLLEGFKQATKKAYSSVLKPVEGTILTVIRETTEALEKEVNNKTSLEEFFKLAKNAARKACDNTPNKLKILREVGVTDSGGEGLYTIISGMEAALNAEAIKISESEENIDTFISSKEVYDGEFGYCTEFIIELNDEKDFDKANFEKSLLKKANSLVVVQDEKIVKVHGHTLKPGDMLNFGQKYGEFIKIKSENMTRQASESRNKNVIINQNDEQSNKKCAIISCNLGSGIIARMKEFGVDAIIESGQTQNPSAQDIIDAINSVNSKNVFILPNNSNIFLAAEQAAQAITNKKVFIIQTRSQIQGLTALMNFNSESSPKENRETMSESIKLVTTAEVTQAIRSTKIDNVKIKEGQFIGITDGKILVSENNYIDAAKKLIKKSINSDHELVTVYYGNDASEVDANEIKNFLESHYDVEVEVVNGAQPNYHFIIGFE", "aa_hexdigest": "daf6d8778c82e3384ca53ddab710e206", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001A8C31B", "ncbi_nrp_id": "WP_013354744.1", "uniref100_id": "UniRef100_C4XDU6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C31B", "RefSeq:WP_013354744.1", "UniRef:UniRef100_C4XDU6" ] }, "ips": { "uniref100_id": "UniRef100_C4XDU6", "uniref90_id": "UniRef90_C4XDU6" }, "psc": { "uniref90_id": "UniRef90_C4XDU6", "product": "DhaL domain-containing protein", "uniref50_id": "UniRef50_A0A449B6P5" }, "pscc": { "uniref50_id": "UniRef50_A0A449B6P5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B6P5" ], "product": "Dihydroxyacetone (Glycerone) kinase, DhaK2 subunit" }, "genes": [ "dhaL" ], "id": "OAIKOHHJFB_155", "locus": "GCA000209735_00110" }, { "type": "cds", "sequence": "contig_1", "start": 56921, "stop": 57211, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013354745.1", "SO:0001217", "UniParc:UPI0001A8C31C", "UniRef:UniRef100_C4XDU7", "UniRef:UniRef50_C4XDU7", "UniRef:UniRef90_C4XDU7" ], "nt": "ATGACTTTAGAAGAAATTAGAAAAGTTTTTTTGAATATTCTTGAATCAACGCCGGGGGTTTATGGAATCTGTGAAAATGCAGAACAATGTCCTAATTTAGGCCAAGAATATTTAAGTATTCAAAATATAAACAATGAATGGCATTTCAGCGCTACAATTATTATTTTAAAATATGCTAATGCAAAAAATGTTGTTAACTCAATTAGTTCTCACTTAAGATACGAACTCAATAAGAAAAAGGAACATTTAGGCAAATTAAATGTATTTATAGGAGGTCTTGCAAATGACTAA", "aa": "MTLEEIRKVFLNILESTPGVYGICENAEQCPNLGQEYLSIQNINNEWHFSATIIILKYANAKNVVNSISSHLRYELNKKKEHLGKLNVFIGGLAND", "aa_hexdigest": "3a51432363c8cd1ca400ef9de6aa183e", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C31C", "ncbi_nrp_id": "WP_013354745.1", "uniref100_id": "UniRef100_C4XDU7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C31C", "RefSeq:WP_013354745.1", "UniRef:UniRef100_C4XDU7" ] }, "ips": { "uniref100_id": "UniRef100_C4XDU7", "uniref90_id": "UniRef90_C4XDU7" }, "psc": { "uniref90_id": "UniRef90_C4XDU7", "uniref50_id": "UniRef50_C4XDU7" }, "pscc": { "uniref50_id": "UniRef50_C4XDU7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDU7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10877.31659999999, "isoelectric_point": 6.275900077819824 }, "id": "OAIKOHHJFB_156", "locus": "GCA000209735_00111" }, { "type": "cds", "sequence": "contig_1", "start": 57345, "stop": 57887, "strand": "+", "frame": 3, "gene": "recO", "product": "DNA repair protein RecO", "db_xrefs": [ "GO:0003677", "GO:0006281", "GO:0006310", "KEGG:K03584", "SO:0001217", "UniRef:UniRef50_C4XDU8", "UniRef:UniRef90_C4XDU8" ], "nt": "ATGGCTGAAAAAATTTTACAAGTTGTTGTTTTAGACATTCAAAATGCTAATACAAGTGATAATGATGCTATTGTTCAAGTTTATTCAAAAAATGGAATTTTCTCACTTTTAGCAAAAGGTGTAAATAAAAGTGAGTCAAAAAATCGAGTTAACTTACAAATAGGATCCTTAGTTGAAATTGAATATTTTCAAGCTCGATTGAACAACAAAGTGAGCTTGCTTAAAAAAGCCACTTTAATTTCAGACATAGATTATTCAAATCGTTTTAATTTAATTTTTATTCAAAAAGCAGTTAATTTATTAAAGCATTTTCCTAAGCCTTATTACACATTATTTGAAACTTATGTTAACTGTTTAAATTACTTAGGATTAGGCAAAAATTCAATGCTTTATACTTATCTTTTAGCTCATACTTTAAACTATTTTGGACTAGATGTAAATGTAAACAAATGTTGTGAATGCAATAGTCCAAGCAACTTGTGTGATTTTAAATTTTATAAAGGTGGATTTTTGTGCGGTAACCACATGAGTGAGCAACGTTGA", "aa": "MAEKILQVVVLDIQNANTSDNDAIVQVYSKNGIFSLLAKGVNKSESKNRVNLQIGSLVEIEYFQARLNNKVSLLKKATLISDIDYSNRFNLIFIQKAVNLLKHFPKPYYTLFETYVNCLNYLGLGKNSMLYTYLLAHTLNYFGLDVNVNKCCECNSPSNLCDFKFYKGGFLCGNHMSEQR", "aa_hexdigest": "bfc34cb513bf9df5df8f4a04af468ad4", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "recO" ], "truncated": "3-prime", "pseudogene": { "start": 57345, "stop": 58025, "observations": { "mutation": [ 57885 ], "stop": [ 57885 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDU8", "query_cov": 1.0, "subject_cov": 0.7964601769911505, "identity": 1.0, "score": 362.0, "evalue": 3.42e-126, "gene_start": 1, "gene_end": 180, "reference_start": 1, "reference_end": 180, "reference_sequence": "MAEKILQVVVLDIQNANTSDNDAIVQVYSKNGIFSLLAKGVNKSESKNRVNLQIGSLVEIEYFQARLNNKVSLLKKATLISDIDYSNRFNLIFIQKAVNLLKHFPKPYYTLFETYVNCLNYLGLGKNSMLYTYLLAHTLNYFGLDVNVNKCCECNSPSNLCDFKFYKGGFLCGNHMSEQRWTKELKSIYYMYNDLKIFLQICISKVNQTIFYELNKYLEENGIYLN" }, "paralog": false, "identity": 0.995575221238938, "score": 455.0, "evalue": 3.75e-165, "description": "Internal stop codon at 57885. Nonsense mutation around 57885.", "psc": { "uniref90_id": "UniRef90_C4XDU8", "gene": "recO", "product": "DNA repair protein RecO", "uniref50_id": "UniRef50_C4XDU8", "kegg_orthology_id": "K03584", "go_ids": [ "GO:0003677", "GO:0006281", "GO:0006310" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDU8", "UniRef:UniRef50_C4XDU8", "KEGG:K03584", "GO:0003677", "GO:0006281", "GO:0006310" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDU8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDU8" ], "product": "DNA replication/recombination mediator RecO N-terminal domain-containing protein" } }, "id": "OAIKOHHJFB_157", "locus": "GCA000209735_00112" }, { "type": "cds", "sequence": "contig_1", "start": 58208, "stop": 58810, "strand": "+", "frame": 2, "gene": null, "product": "Nitroreductase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDU9", "UniRef:UniRef90_C4XDU9" ], "nt": "ATGTCTTTTATTGAAAATAATTTAAAAAGAGGATCAACAAGAGATTATCAAATTGGCTATGAAATTAGTGAAGAAGATAAAGAAAAATTGATCAAAACTATTCAAAATGCACCAACAAGTAACAACTATTTTGCTTCTTCAGTTATTGTAATTGAAGACCAAAATACTAAGGACAAATTAGCTGAAATTTTACAACAAAAACAAGTTAGAGAATGCTCAATGTTTTTTATCTTTTTAGCTGACAATAATCGTATTGATTATGTCTTAAAAACTAAAAATAAAAATATTGAAAATAAAACTCTCAACTCATTATTACTTTCAGTTGGAGATGCTTTTATTCAAGCAACAATGCTTCAAGATGCTGCTACTGAATTGGATTTAGGAACATGCTTTATTGGAGGAGTTAGAGGACACATTAGTGAAATCAATAGATTACTTAAAATCAAAAACTCAGCTTTTCCAGCAGTTGCTTTAACAGTTGGTAAAGCAGCTAAAAAAGCAGATCTTAAACCAAAAATTAATAGAATTTACTATGAACAATATTCATATGCACAAGTTGTTGATGAAATTGTTCCATATAATGAAAAATTAACAAACTATTGA", "aa": "MSFIENNLKRGSTRDYQIGYEISEEDKEKLIKTIQNAPTSNNYFASSVIVIEDQNTKDKLAEILQQKQVRECSMFFIFLADNNRIDYVLKTKNKNIENKTLNSLLLSVGDAFIQATMLQDAATELDLGTCFIGGVRGHISEINRLLKIKNSAFPAVALTVGKAAKKADLKPKINRIYYEQYSYAQVVDEIVPYNEKLTNY", "aa_hexdigest": "ba2d98a50dfbad4f7a08c7e2fc3e9f55", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_C4XDU9", "query_cov": 1.0, "subject_cov": 0.8403361344537815, "identity": 1.0, "score": 386.0, "evalue": 4.21e-135, "valid": true, "product": "Nitroreductase domain-containing protein", "uniref50_id": "UniRef50_C4XDU9" }, "pscc": { "uniref50_id": "UniRef50_C4XDU9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDU9" ], "product": "Nitroreductase domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_158", "locus": "GCA000209735_00113" }, { "type": "cds", "sequence": "contig_1", "start": 58993, "stop": 59136, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAATTTAAACGTGTTTTTATGATTGTTACAGATGGTCTAGGAATTGGACCAGATAAAGATCAAAGAGCTTTTGGTGACAAAGGCGCTAACACAATTCTTTCAGCTTCAAAAAGCTCAATGTTCTTTATTGACACATGA", "aa": "MAKFKRVFMIVTDGLGIGPDKDQRAFGDKGANTILSASKSSMFFIDT", "aa_hexdigest": "073303c3d294ddbd4feec3b74c7ef7c5", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5113.885799999999, "isoelectric_point": 9.397443580627442 }, "id": "OAIKOHHJFB_159", "locus": "GCA000209735_00114" }, { "type": "cds", "sequence": "contig_1", "start": 59263, "stop": 59496, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGGGAATTAAAACAATGGTTCCATTCCCTACATTTACTGAAAATGGTTTCCCTCAAGATTTATTAGATGAATTATCTAAAGCTTTTGATGGTCGTAAAATTATTTGCAACAAATCAGGTTCAGGTACTGAAATGATTGATGAATATGCAGAACAACAAAAGAAAGATGGTTCAATTATTGTTTATACATCAATGGACTCAGTATTACAAATTGCAGCTCATGAAGAATGA", "aa": "MMGIKTMVPFPTFTENGFPQDLLDELSKAFDGRKIICNKSGSGTEMIDEYAEQQKKDGSIIVYTSMDSVLQIAAHEE", "aa_hexdigest": "6b70b7a38edd571932330ad7b6fe5ca5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8577.6579, "isoelectric_point": 4.5660696029663095 }, "id": "OAIKOHHJFB_160", "locus": "GCA000209735_00115" }, { "type": "cds", "sequence": "contig_1", "start": 59662, "stop": 60126, "strand": "+", "frame": 1, "gene": null, "product": "Phosphopentomutase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1C5TNN4" ], "nt": "ATGATTCTAAATGAATTACAAAAAGCTGGTGTTAATGTTATTGGTATTGGTAAAATCTTTGACATTTTCGTAGGTCAAGGTATTAGTGAATCGCTTCACTCAGATGGTGATGCTCATGGTATGGACCTTACAATTAAATGTGCAGAAGAAAGACCAGAAAACACATTTGTATTCACAAACTTAGTTCAATTTGACTCGCACTATGGACACCGTAGAGATGTTGATGGTTATGCTTCAAACATTGCATTACTTGACTCAAAATTAGGTAAATTAATTAATGCTATGAAAGAAGACGACTTATTAATTATAACAAGTGACCACGGTAATGATCCTCTTTATCCAGGATTCAACCACACTCGTGAATTACTTCCTTTAACAATCTTTTCAAAAAGATTCAAAAAACCTAAAGTTCTTGAAGATGTTCTTGGTTTAGGAACAAGTGGAAATATTGTTGCCCGTAACTGA", "aa": "MILNELQKAGVNVIGIGKIFDIFVGQGISESLHSDGDAHGMDLTIKCAEERPENTFVFTNLVQFDSHYGHRRDVDGYASNIALLDSKLGKLINAMKEDDLLIITSDHGNDPLYPGFNHTRELLPLTIFSKRFKKPKVLEDVLGLGTSGNIVARN", "aa_hexdigest": "cd97e1534fa4d8b09c15c4b3354eb5a3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1C5TNN4", "query_cov": 0.8961038961038961, "subject_cov": 0.8518518518518519, "identity": 0.5, "score": 146.0, "evalue": 4.76e-42, "valid": false, "product": "Phosphopentomutase", "ec_ids": [ "5.4.2.7" ], "uniref50_id": "UniRef50_A0A1C5TNN4", "kegg_orthology_id": "K01839" }, "pscc": { "uniref50_id": "UniRef50_A0A1C5TNN4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1C5TNN4" ], "product": "Phosphopentomutase" }, "genes": [], "id": "OAIKOHHJFB_161", "locus": "GCA000209735_00116" }, { "type": "cds", "sequence": "contig_1", "start": 60268, "stop": 61056, "strand": "+", "frame": 1, "gene": "whiA", "product": "DNA-binding protein WhiA", "db_xrefs": [ "GO:0003677", "GO:0043937", "SO:0001217", "UniRef:UniRef50_C4XDV1", "UniRef:UniRef90_C4XDV1" ], "nt": "ATGAACTTTACTAAATTCGTTAAAAATGAAATTATCAATCGCAAAAAAAATGAAGTTGAATCTCTTGAATACTTAAGAGGTTTAATTTTTGGAAATGGCTTAATTAAAAATGATTCCATTCATTTAAACATTCGTGATGAAGAATTTTTTTATTTAATAATTGAATTAATTAAAAAAGTAAACTTAGATTATCAAATTAAAAATTCTAAAACAATAGTTTTAAATAAAAAAGATATTGACTTAGATGTAAAATTTTTAAAGCCTTCTTTATTCTTTGGTGGAGTATTTCAAACAGGTGGAAGTATTAGCAATCTTGGTAAAACTTCATACCACTTGCAATTAAGCTCAAACTATGAAAATTTTATAGATATTATTATGAGCAAATTGAATGAATATTACTTTAATTTTCAAAAATTAAAACACCAAAATAAATACATAATTTATGTCAAACAAAAAGAAAAAATTGAAGATTTTTTGAAAGCAATTTTAGCTTTAGAATCATTTTATAAATTTACCGATATTACGATAAAAAGGGACTTTGAGAATAGTTTTAATCGAATAAATAACATAGATATTTATAATATTAAAAAGGCAGTGGAAGCTAATGTAAAACATATTGAAAACTTAAATTATATTTTTGAAAACAAACTTGAAAATAAATTCAAAGATGAGCAAATATTACTTTATAAAACAGTTCTAAATAATCCAGAAGCGCCTTTATCTAACATCATTTTATCACTTGAAAAAGAGCATAATTTAATTAAAAGCAAAAGCACAATTCATCATTGA", "aa": "MNFTKFVKNEIINRKKNEVESLEYLRGLIFGNGLIKNDSIHLNIRDEEFFYLIIELIKKVNLDYQIKNSKTIVLNKKDIDLDVKFLKPSLFFGGVFQTGGSISNLGKTSYHLQLSSNYENFIDIIMSKLNEYYFNFQKLKHQNKYIIYVKQKEKIEDFLKAILALESFYKFTDITIKRDFENSFNRINNIDIYNIKKAVEANVKHIENLNYIFENKLENKFKDEQILLYKTVLNNPEAPLSNIILSLEKEHNLIKSKSTIHH", "aa_hexdigest": "4079e155a7f43b90c638e0046b8a8881", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XDV1", "query_cov": 1.0, "subject_cov": 0.9390681003584229, "identity": 1.0, "score": 507.0, "evalue": 4.52e-181, "valid": true, "gene": "whiA", "product": "DNA-binding protein WhiA", "uniref50_id": "UniRef50_C4XDV1", "go_ids": [ "GO:0003677", "GO:0043937" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDV1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDV1" ] }, "genes": [ "whiA" ], "id": "OAIKOHHJFB_162", "locus": "GCA000209735_00117" }, { "type": "cds", "sequence": "contig_1", "start": 61107, "stop": 61565, "strand": "+", "frame": 3, "gene": null, "product": "DUF86 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDV2", "UniRef:UniRef90_C4XDV2" ], "nt": "ATGAAAGAGCGAATGAAATATCTTAACATAAAAATTCAAGAAAAAATGAATCACATTCACTATTTAACTGGAACTGTTGTTCATAGTGTACAAAAACTTGAATATCGTTTAACATATTTAATCACTTTAAAAAAAACAATCGATGAATATGAAAAAAAAGGTAAATTTAAACATAATTTATTTTTAAAAGTTGTGCTAGAAGCATCTTCTGAAGCTCAAAAAATCTTTAGAAGTTTGTATGAAAATACTTTTGGTAATTTAAGCCAAAAAGCCTATCAAAATGGCATTTTGAGCAAAAATGAACATTTAGAATTATCTAAAGTTGTTGAAGATCGCAATGAATTAATTCATTTCTTTTTTAAGGATATTGAATTTAAACAAACCGATGATCAATTAGCTTTATATCTTGATGAAAGAATTAATTTTTTAGAAGATCTTTTTTCAAGAACTAGACGATGA", "aa": "MKERMKYLNIKIQEKMNHIHYLTGTVVHSVQKLEYRLTYLITLKKTIDEYEKKGKFKHNLFLKVVLEASSEAQKIFRSLYENTFGNLSQKAYQNGILSKNEHLELSKVVEDRNELIHFFFKDIEFKQTDDQLALYLDERINFLEDLFSRTRR", "aa_hexdigest": "a3620180200999e4e1966a354983dd62", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_C4XDV2", "query_cov": 1.0, "subject_cov": 0.9156626506024096, "identity": 1.0, "score": 295.0, "evalue": 9.02e-101, "valid": true, "product": "DUF86 domain-containing protein", "uniref50_id": "UniRef50_C4XDV2" }, "pscc": { "uniref50_id": "UniRef50_C4XDV2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDV2" ], "product": "DUF86 domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_163", "locus": "GCA000209735_00118" }, { "type": "cds", "sequence": "contig_1", "start": 61657, "stop": 62622, "strand": "+", "frame": 1, "gene": "holA", "product": "DNA polymerase III subunit delta", "db_xrefs": [ "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0006260", "RefSeq:WP_013526971.1", "SO:0001217", "UniParc:UPI0001A8C322", "UniRef:UniRef100_C4XDV3", "UniRef:UniRef50_A0A3P8KAF3", "UniRef:UniRef90_C4XDV3" ], "nt": "ATGAATTTTATTTATGGCTCAGAAAATTTTTTTATTGAACAAGAAATCAATAAAATCGTTAAAAAATTTCCAAATTCTGCTATTACAACATTTAACATTGAAAACGATGAAAATGTTGATAGTTTAATTCAATTTATAAGCAGTGGAGATTTGTTTAATAAAAAAAGAATCATTGTAATTAATGATTTATTTTACTTTGAAAAAAAATTAAGCTCAAATGATGAAACTAATGTTAATAATTTAATTAAAAGTATTGACAATAATTCAAGTGATGAATTAATTTTTGTAAATACAAACATAAGCAATAAAGAGCAAATAAATAAAAACTTTTTTACTAATTTTATTTTCAACAAATTGCAGCAAAATAATTTTATTGAAGCTAATAGTATTGATGATAATCAATTAACTAAAAAAGTTGTTTCATTAGTACAACAAAAAGGCGGTACTATTGATGCTTTAGCAACCAGCGCTTTACTTAAAAAAATACCAAATGATCTTTATTTAATTAACTTAGAAATTGATAAATTAATTAATCAAAATAAACACATAACTGAAAATATGATTAATGTTTCAGTAATTGATATTTATGTAGAAGACGCTTTTGGTTTTAGCAATAGTTTTGAAACAAATGATTTCAATCTTATTTGGAAAAAATATAAAGAAAAATTAATTGAAGGAGTTGATGTTACTGTTTTAATTGCACAAACTTCACAATTATTTATTTTAGCTAACCAAATTTATGCATATAAAAATATAGGTAAAACTTTAGAAAATTTAGCAAGCGATTTTAAAATTAATCAATATAGAGTTAAAAAAGTCAGTTATTTATTAACAAGACTTGGCACTTTAAAAATTAGAAGAATGATTAAATCTCTTGCAAAACTTGACCAAGATATAAAAGAAGGAAAAACTGATCCAACAATTGGATTTGAAAGGTTCTTAATAAAATTCTTTATTAATGGATAA", "aa": "MNFIYGSENFFIEQEINKIVKKFPNSAITTFNIENDENVDSLIQFISSGDLFNKKRIIVINDLFYFEKKLSSNDETNVNNLIKSIDNNSSDELIFVNTNISNKEQINKNFFTNFIFNKLQQNNFIEANSIDDNQLTKKVVSLVQQKGGTIDALATSALLKKIPNDLYLINLEIDKLINQNKHITENMINVSVIDIYVEDAFGFSNSFETNDFNLIWKKYKEKLIEGVDVTVLIAQTSQLFILANQIYAYKNIGKTLENLASDFKINQYRVKKVSYLLTRLGTLKIRRMIKSLAKLDQDIKEGKTDPTIGFERFLIKFFING", "aa_hexdigest": "75553d84ac2538df7f415568daa5558a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C322", "ncbi_nrp_id": "WP_013526971.1", "uniref100_id": "UniRef100_C4XDV3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C322", "RefSeq:WP_013526971.1", "UniRef:UniRef100_C4XDV3" ] }, "ips": { "uniref100_id": "UniRef100_C4XDV3", "uniref90_id": "UniRef90_C4XDV3" }, "psc": { "uniref90_id": "UniRef90_C4XDV3", "gene": "holA", "product": "DNA polymerase III subunit delta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_A0A3P8KAF3", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KAF3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KAF3" ], "product": "DNA-directed DNA polymerase" }, "genes": [ "holA" ], "id": "OAIKOHHJFB_164", "locus": "GCA000209735_00119" }, { "type": "ncRNA", "class": [ "RNase_P_RNA", "SO:0000386" ], "sequence": "contig_1", "start": 63372, "stop": 63644, "strand": "+", "gene": "RNaseP_bact_b", "product": "Bacterial RNase P class B", "score": 209.8, "evalue": 2.6e-25, "db_xrefs": [ "RFAM:RF00011", "SO:0000386" ], "nt": "AGCATTGGCTAACAGCTGCTTGAAAGAGTAGAGGAAAGTCCACGCTAGCACAACCTGCGATGGTTGTAGTGATCATGCTAGGCCCAATAAGCCTAGGCTTAGACGACTAGTGCCACAGAGACGAGAATTGTGAAACGCGGTAAACTCCATGAGTTAGAAACCCAAATATTGGTAGGGGAACTTTTCAACGGAAATTGAACCAAAGAAAAGAGTTGATTAATCAACTAGATAAATTGTTAGCACCTGAGAGGGTACAAAACGTGGCTTATAAAT", "id": "OAIKOHHJFB_42", "locus": "GCA000209735_00120" }, { "type": "cds", "sequence": "contig_1", "start": 63736, "stop": 63858, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013354753.1", "SO:0001217", "UniParc:UPI0001E32EB1", "UniRef:UniRef100_A0AB32XC49", "UniRef:UniRef50_A0AB32XC49", "UniRef:UniRef90_A0AB32XC49" ], "nt": "ATGAGTGTAATTGATAAAATTAAAGAATTAATAAAAGAAAACAATGGTTATATGTCCACAGCAATTTTAGATAAAAACAAAATTTCAAGAAATTATTTGAAATTCTTAATTGAAAAAAATTAA", "aa": "MSVIDKIKELIKENNGYMSTAILDKNKISRNYLKFLIEKN", "aa_hexdigest": "180a7bcc61b14991a91355f416da1c11", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32EB1", "ncbi_nrp_id": "WP_013354753.1", "uniref100_id": "UniRef100_A0AB32XC49", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EB1", "RefSeq:WP_013354753.1", "UniRef:UniRef100_A0AB32XC49" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XC49", "uniref90_id": "UniRef90_A0AB32XC49" }, "psc": { "uniref90_id": "UniRef90_A0AB32XC49", "uniref50_id": "UniRef50_A0AB32XC49" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XC49", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XC49" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4716.521099999999, "isoelectric_point": 9.456109809875489 }, "id": "OAIKOHHJFB_165", "locus": "GCA000209735_00121" }, { "type": "cds", "sequence": "contig_1", "start": 64115, "stop": 64315, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDV6", "UniRef:UniRef90_C4XDV6" ], "nt": "ATGTTTGGCAATTATGCTAAAACATACAATAAAGAAAGAATAATTTGTGACTTAATTTTGGATATCAATAAATATGAACCAAAATTATGTCAAACGACTATAGAACTATATAAAGATTCAAAGCATGATTTGAAAAGACTTCTTAAATATGCAAAGATTTTTAATATAGTTGAAAGAATAAAATTGTTGTTTAATTTATAA", "aa": "MFGNYAKTYNKERIICDLILDINKYEPKLCQTTIELYKDSKHDLKRLLKYAKIFNIVERIKLLFNL", "aa_hexdigest": "9db485b1af58dc8b45d309d01aa42a9d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 63881, "stop": 64315, "observations": { "mutation": [ 63893, 63905, 63908, 63953, 64079 ], "stop": [ 63893, 63905, 63908, 63953, 64079 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDV6", "query_cov": 1.0, "subject_cov": 0.4583333333333333, "identity": 1.0, "score": 135.0, "evalue": 1.07e-39, "gene_start": 1, "gene_end": 66, "reference_start": 79, "reference_end": 144, "reference_sequence": "MCRYWYCRWWTLCISIEIQKVHFFWISAMFLQKITNVLYHRTEANFSTNYNTSKIDKKIKCHRIIDWKLNLGVEKVKIMFGNYAKTYNKERIICDLILDINKYEPKLCQTTIELYKDSKHDLKRLLKYAKIFNIVERIKLLFNL" }, "paralog": false, "identity": 0.9583333333333334, "score": 276.0, "evalue": 2.3e-97, "description": "Internal stop codon at 63893, 63905, 63908, 63953, 64079. Nonsense mutation around 63893, 63905, 63908, 63953, 64079.", "psc": { "uniref90_id": "UniRef90_C4XDV6", "uniref50_id": "UniRef50_C4XDV6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDV6", "UniRef:UniRef50_C4XDV6" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDV6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDV6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7998.4307999999955, "isoelectric_point": 9.329042625427245 }, "id": "OAIKOHHJFB_166", "locus": "GCA000209735_00122" }, { "type": "cds", "sequence": "contig_1", "start": 64467, "stop": 65354, "strand": "+", "frame": 3, "gene": "ychF", "product": "redox-regulated ATPase YchF", "db_xrefs": [ "GO:0005524", "GO:0005525", "GO:0016887", "SO:0001217", "UniRef:UniRef50_A0A3D5Z7W8", "UniRef:UniRef90_C4XDV7" ], "nt": "ATGTCATTAAAAGCTGGTATTGTTGGATTACCAAATGTTGGAAAAAGTACACTTTTTAGTGCTTTAACTAAATATCAAGTTGAAGCTTCTAATTATGCTTTTACAACAATTGAACCAAATATTAGTAGTGTCCCGCTTAAAGATCCAAGATTATATGAATTAGCAAAGATAGTTAAACCTGGAAGAATTGTACCTGCAACTTTTGACTTCGTTGATATTGCAGGTTTAGTTAAAGGTGCAAGTAAAGGTGAAGGCTTAGGCAATAAATTTTTAGGTAATATTCGTGAAGTCGATGCTGTTGTGCATGTAGTTAGATGTTTTGATGATAAAAATATAATGCATGTGGCTAATGAAGTTAATCCAGTTAATGATAAAGATGTTATTAATATGGAATTAATGTTAGCTGATTTAGAAACCATTAATAATATTATTAATAGAATTCACAAAAAAGCTAAAAGTGGTGACAAGGAAGCTATTTTAGAAGAAAATTTAGCTTTAAAAATCAAAGATCTTTTAGAAAATAATAAACCAGCAAGAATGATTAAAAATTTAAGTGAAGATGAAGAAAAATTAATTAAAAATTATCATCTTTTAACTTCAAAACCAATGATTTATGTTGCTAATATGTCAGCTGAAGAAATAGCAGAGTATGAAAATGCTCCTTTATATAAACAATTAAAAGTTAGTGTTGAAGACGATGTAAAAATTTTGCCTATTTGTGTTCAACTCGAAAGCGAAATTAGCCAAATGGATGAAAGTGAAAGCAAAGAATGGTTAGATAGCTACAACATTAAATTTAGTGGTTTAGACCTTCTTGCTAGAGAAGCTTTTGATTTGTTAAAACTTAAAACTTATTTTACAGCTGGTGAAATGGAAGTTAAAGCTTGA", "aa": "MSLKAGIVGLPNVGKSTLFSALTKYQVEASNYAFTTIEPNISSVPLKDPRLYELAKIVKPGRIVPATFDFVDIAGLVKGASKGEGLGNKFLGNIREVDAVVHVVRCFDDKNIMHVANEVNPVNDKDVINMELMLADLETINNIINRIHKKAKSGDKEAILEENLALKIKDLLENNKPARMIKNLSEDEEKLIKNYHLLTSKPMIYVANMSAEEIAEYENAPLYKQLKVSVEDDVKILPICVQLESEISQMDESESKEWLDSYNIKFSGLDLLAREAFDLLKLKTYFTAGEMEVKA", "aa_hexdigest": "bb607f6fba9700308db2b1169e322269", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_C4XDV7", "query_cov": 1.0, "subject_cov": 0.8060109289617486, "identity": 1.0, "score": 565.0, "evalue": 4.06e-202, "valid": true, "gene": "ychF", "product": "redox-regulated ATPase YchF", "uniref50_id": "UniRef50_A0A3D5Z7W8", "go_ids": [ "GO:0005524", "GO:0005525", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3D5Z7W8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D5Z7W8" ], "product": "Ribosome-binding ATPase YchF" }, "genes": [ "ychF" ], "id": "OAIKOHHJFB_167", "locus": "GCA000209735_00123" }, { "type": "cds", "sequence": "contig_1", "start": 65370, "stop": 65567, "strand": "+", "frame": 3, "gene": "ychF", "product": "YchF C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0P9MY34" ], "nt": "ATGTTAGCTCCTGCTTGTGCTGGCATAATTCACTCAGATTTTGAAAAGAAATTTATTAAAGCAGATGTAATTAGTTATCAAGATTACATTGATAATAATGGCGAATTAGGCGCTCGTAATGCAGGAAAAATTAGATCAGAAGGTAAAAATTATGTAATGCAAGATGGCGATATTTGTCACTTTAAATTTGGCAAATAA", "aa": "MLAPACAGIIHSDFEKKFIKADVISYQDYIDNNGELGARNAGKIRSEGKNYVMQDGDICHFKFGK", "aa_hexdigest": "332c967efb390a89ade3681ad845c738", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A378MX97", "query_cov": 0.9384615384615385, "subject_cov": 0.8591549295774648, "identity": 0.639, "score": 92.0, "evalue": 2.89e-23, "valid": false, "product": "GTP-dependent nucleic acid-binding protein engD", "uniref50_id": "UniRef50_A0A0P9MY34" }, "pscc": { "uniref50_id": "UniRef50_A0A0P9MY34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0P9MY34" ], "product": "YchF C-terminal domain-containing protein" }, "genes": [ "ychF" ], "id": "OAIKOHHJFB_168", "locus": "GCA000209735_00124" }, { "type": "tRNA", "sequence": "contig_1", "start": 65579, "stop": 65652, "strand": "+", "gene": "trnW", "product": "tRNA-Trp(cca)", "amino_acid": "Trp", "anti_codon": "cca", "score": 65.6, "nt": "AGGGGTTTAGTATAATGGTAGTACCGCAGTCTCCAAAACTGCTCGCATGGGTTCGATTCCTATAACCCCTGCCA", "db_xrefs": [ "SO:0000271" ], "anti_codon_pos": [ 65611, 65613 ], "id": "OAIKOHHJFB_1", "locus": "GCA000209735_00125" }, { "type": "cds", "sequence": "contig_1", "start": 66103, "stop": 66213, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGGACCTTTTACCTTATTTATTATTTTAATAGTTGCAGCTATTTTATTAAAAATAGAAATTAAAGAAAACACAAGATTTGCAACAAGAGTTACAGGATATCGTTGA", "aa": "MFGPFTLFIILIVAAILLKIEIKENTRFATRVTGYR", "aa_hexdigest": "d709db95fa6de6d389cb775ec6b87363", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4157.017100000002, "isoelectric_point": 10.266412925720214 }, "id": "OAIKOHHJFB_169", "locus": "GCA000209735_00126" }, { "type": "cds", "sequence": "contig_1", "start": 66960, "stop": 67277, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGGTGATGGTGTTAATGATGCTCCCGCTCTTAAAACTAGTGACATTGGTTGTGCTATGGGAATTACAGGAACTGAAGTTTCAAAACAAGCAGCTGATTTAATCTTAGTTGACGATAACTTCAATACTATTGTTAATTCAGTAAGAAATGGTCGTTTAATTTTTGACAAAATCAAAACAGTGATACTTAACTTATTAATTAGCTCACTAACAGAAATTTTAGTAATGCTTATTGGTATGGTCGCTTTCTTCTTAGCATATAAAGATTATTATCCAGATGGATTTTACATTTTTGGAGCTAGCCAATTATTATGA", "aa": "MTGDGVNDAPALKTSDIGCAMGITGTEVSKQAADLILVDDNFNTIVNSVRNGRLIFDKIKTVILNLLISSLTEILVMLIGMVAFFLAYKDYYPDGFYIFGASQLL", "aa_hexdigest": "4bc9ea45c1223c7fa7907418ac6d2104", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11385.136099999992, "isoelectric_point": 4.392767524719238 }, "id": "OAIKOHHJFB_170", "locus": "GCA000209735_00127" }, { "type": "cds", "sequence": "contig_1", "start": 67302, "stop": 67568, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00702.32" ], "nt": "ATGATTGATCCACCTCGGGCAGAGGTTGCAAATTCAATTAGAGAAGCTCAAGGTGCTGGTATTAAAGTTGTTATGATCACAGGTGATCAAGTAACTACAGCAAGAGCTATAGCTCAAAATTTAGGCATTTATCAAGAAAGCGATACTTGTCTTGAAGGCAAAGATTTAGCCAAAATGAGTGAAGAAGAATTAAGAGCTAAAGTTGAACATATTTCAGTTTATGCTCGTGTTAATCCTGAAGACAAATTAAGAATTGTTCAAGCATGA", "aa": "MIDPPRAEVANSIREAQGAGIKVVMITGDQVTTARAIAQNLGIYQESDTCLEGKDLAKMSEEELRAKVEHISVYARVNPEDKLRIVQA", "aa_hexdigest": "0a8369a165fe0e7449d5ec3d92cfbd6b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00702.32", "name": "Hydrolase", "length": 85, "aa_cov": 0.9431818181818182, "hmm_cov": 0.8117647058823529, "evalue": 7.2459971360409805e-09, "score": 32.37348937988281, "start": 3, "stop": 85 } ], "seq_stats": { "molecular_weight": 9651.904999999993, "isoelectric_point": 5.089272499084472 }, "id": "OAIKOHHJFB_171", "locus": "GCA000209735_00128" }, { "type": "cds", "sequence": "contig_1", "start": 67674, "stop": 67823, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTTTCGATTCAAATCGTAAAATGCACTCTGTAGTATTCAAAGAAAAAGATAGAATGCTTTTAGTTACTAAAGGTGCTCCTGATGTTTTATTAAAAAGATGTAATAATTTAACTATTGATGATATTAAAGAAATTAATCACCAATGA", "aa": "MPFDSNRKMHSVVFKEKDRMLLVTKGAPDVLLKRCNNLTIDDIKEINHQ", "aa_hexdigest": "5574b6ef4dc0134333c5777e7845c54c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5739.6747, "isoelectric_point": 8.978850364685059 }, "id": "OAIKOHHJFB_172", "locus": "GCA000209735_00129" }, { "type": "cds", "sequence": "contig_1", "start": 68022, "stop": 68165, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCCAAACAAAAAGGGTTGGTTAAAAACCTTTTAGCAGTTGAAACCCTAGGTTCTGCTTCAATTATTTGTAGTGATAAAACAGGAACACTAACAGAAAATAAAATGACAGTAGTTGATGCTTATCGTTTTAGAACTCTTTGA", "aa": "MSKQKGLVKNLLAVETLGSASIICSDKTGTLTENKMTVVDAYRFRTL", "aa_hexdigest": "7cff828fbd3b514d5e27dff5fb1ad487", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5132.972300000001, "isoelectric_point": 9.428839683532715 }, "id": "OAIKOHHJFB_173", "locus": "GCA000209735_00130" }, { "type": "cds", "sequence": "contig_1", "start": 68364, "stop": 69110, "strand": "-", "frame": 3, "gene": null, "product": "HAD-IC family P-type ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF3AF86" ], "nt": "ATGAACAAAAAAGACTATTCCAATTATCAAGGCTTGACTTCAAAAGAAGTTGAAGCAAACCTTGCAAAGTATGGTGAAAACGCTTTAATTAAAAAGAAAAAACTAAATCCAATAGTTGCTTTCTTTAGACAATTTGTTGATCCAATGGTTATTCTTTTAATCATAGCGGCAGCAATTAGTTTAACTTTGGCAATTGTTGAACATTTTCAAGGCGGAAAAAGCACAACCGATTTAATTATCTCTTATGTTGAACCTGGAATTATTATGCTAGTTATTATTTTAAATAGTATGTTAGGAGCTTATCAAGAGGTCAAAAGTGATCAAGCGGTGCGAGCCCTTGAAAAAGTTAATCAGGTCAATGCTACAGTAATTAGGGATGGCAAAGTCCAAATTATTCCCGCTAATTTATTAGTGCCTGGTGACTTAGTTGTTTTAGCTGCAGGAGATACAATTAATGCTGATGGTCGCTTAATTGAAGCTTCAAACTTAGAAGTTGTAGAAGCTTCATTAACTGGTGAAAGTTTACCAGTAAGTAAAGTCGCAAATTTAAATGCAACTGATGACAAAATCTTAGCTAACAACAAACACTTAGTTTACTCAGGCACTTATGTAATTAATGGACGGGCAACTTTTGTAGTTGAACATACAGCTAGTGAAACTGAAATTGGCAAAATTAATTCAATGATTCAAAAACAAAATAAAAATGCAACACCACTACAAATCAAACTTAATAAATTAAGCAAATGA", "aa": "MNKKDYSNYQGLTSKEVEANLAKYGENALIKKKKLNPIVAFFRQFVDPMVILLIIAAAISLTLAIVEHFQGGKSTTDLIISYVEPGIIMLVIILNSMLGAYQEVKSDQAVRALEKVNQVNATVIRDGKVQIIPANLLVPGDLVVLAAGDTINADGRLIEASNLEVVEASLTGESLPVSKVANLNATDDKILANNKHLVYSGTYVINGRATFVVEHTASETEIGKINSMIQKQNKNATPLQIKLNKLSK", "aa_hexdigest": "4e78b4d62684d3dfb594b35b06e5267a", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0021FFE00D", "query_cov": 1.0120967741935485, "subject_cov": 0.8869257950530035, "identity": 0.502, "score": 222.0, "evalue": 9.12e-69, "valid": false, "product": "cation-transporting P-type ATPase", "uniref50_id": "UniRef50_UPI002FF3AF86" }, "pscc": { "uniref50_id": "UniRef50_UPI002FF3AF86", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF3AF86" ], "product": "HAD-IC family P-type ATPase" }, "genes": [], "id": "OAIKOHHJFB_174", "locus": "GCA000209735_00131" }, { "type": "tRNA", "sequence": "contig_1", "start": 69542, "stop": 69633, "strand": "-", "gene": "trnS", "product": "tRNA-Ser(gct)", "amino_acid": "Ser", "anti_codon": "gct", "score": 73.9, "nt": "GGAACAGTACTCAAGTCGGTCGAAGAGGCGGTCCTGCTAAGACTGTAGGGGTGGCAACACCCGCGGAGGTTCGAATCCTCTCTGTTCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 69596, 69598 ], "id": "OAIKOHHJFB_36", "locus": "GCA000209735_00132" }, { "type": "cds", "sequence": "contig_1", "start": 69832, "stop": 70092, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGCGATACCAACATAAAACTAGGAAATCAAGCAACTGCTTTTAGTGAAATACTAAAAGATCAATATTATAAAGCACTGTTAACTGACTCAAAACAACACAAGAGTTCATTGAGTAATAGTACATGGGGAAAATATTCTGAACCTTATGACAAGATTTTTACTAGAACCAATTTAAAATATGAAAACGCAAACTTCTTTCCTTTATACGATATTTTTAAAGATAATATTCTCAAAGATTATCAAACAATAGAAGAATGA", "aa": "MGDTNIKLGNQATAFSEILKDQYYKALLTDSKQHKSSLSNSTWGKYSEPYDKIFTRTNLKYENANFFPLYDIFKDNILKDYQTIEE", "aa_hexdigest": "d6af4ff35a32557b368bc92d5ce06ce7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10102.138099999993, "isoelectric_point": 5.775148582458496 }, "id": "OAIKOHHJFB_175", "locus": "GCA000209735_00133" }, { "type": "cds", "sequence": "contig_1", "start": 70132, "stop": 70305, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGTTAAATTTAAAACTTTAGGACCAATTCAAAATAATTTCACTTTTGTTATAGATCATTTAGATAGTCCAGGAGCAGCAAAAAGTGGCGAATCAAAATCAAGTTTATTAAGTAAGCAAGGCGCTCAAGAAGCTGATGAAGCTTACAACTTAAAGAACCTTATGGATTGA", "aa": "MGVKFKTLGPIQNNFTFVIDHLDSPGAAKSGESKSSLLSKQGAQEADEAYNLKNLMD", "aa_hexdigest": "5532db168bd029edaecbddbe88526eef", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6142.8354, "isoelectric_point": 5.641122245788575 }, "id": "OAIKOHHJFB_176", "locus": "GCA000209735_00134" }, { "type": "cds", "sequence": "contig_1", "start": 70507, "stop": 70680, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGGAATATTTTAAATTTATATGAAACATCTGAAGAATACAAAAAATATGGTTTAGCTAGTGTTATTGAACATTTAAATCTTGATGTTATTGGGCTTATTGAAAATAAAACAAAAGCTTCAGCACAAAAATTTTGTGAATACTTAAAAAAATTTACGGGAAACAACAATTGA", "aa": "MWNILNLYETSEEYKKYGLASVIEHLNLDVIGLIENKTKASAQKFCEYLKKFTGNNN", "aa_hexdigest": "f427d57dde27cf0904ea9bfeb67a583a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6628.495500000001, "isoelectric_point": 6.543952751159668 }, "id": "OAIKOHHJFB_177", "locus": "GCA000209735_00135" }, { "type": "cds", "sequence": "contig_1", "start": 71482, "stop": 71649, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAAAACAATTTATTAAATTATTGGTAGGGTCTTTTTCTTTAATATCCTTACCTTTTTTATCAACTCAATGTAGCTTTTTTGAAATGAATAAAACACAAGAAAAAAACAAAGATGAAATTCAAAATGACTTAAATAATGCGGTTGAAAAAATCAAAATTTGA", "aa": "MKKKQFIKLLVGSFSLISLPFLSTQCSFFEMNKTQEKNKDEIQNDLNNAVEKIKI", "aa_hexdigest": "1d662c39a195fe31e3711c808814489d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6408.464800000002, "isoelectric_point": 9.252131843566893 }, "id": "OAIKOHHJFB_178", "locus": "GCA000209735_00136" }, { "type": "cds", "sequence": "contig_1", "start": 72168, "stop": 72995, "strand": "-", "frame": 3, "gene": null, "product": "UTP--glucose-1-phosphate uridylyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W5UT80", "UniRef:UniRef90_C4XDW2" ], "nt": "ATGACAAAAATTATTCATAAAGAATTAGTGCCTATTCTTGATACACCTGTTATTGATAGATTAGTTAATGAAGCACTTGATTCAGGTATTGAGGAAATTTTAATTGTTTTAAGTGAAAGGAAAAAGGATTTATTAAAATTTTTCTCAGTTGATCAAGATTTACAAACAGAATTAATTATCAAAGGTAAAAAAGATATTTTAGAAAAAGTTAAAAGAACAAATCGCTCTTCATGTATTACTAGAGTAATTCAAAAGGAACAATTTGGTTTAGGTCATGCACTTGCAAGTTGTAAAGAATATATTGACAATGAGCCGTTTGCTATTATTTTAGGTGATGACTTAATTAAATCAAAAACTCCTGCTATTAAGCAATTAATCGAGTTTTATAATAAAACTGGAGCAAATGTTATTGGTGTACAATCAGTAGCAAAAGAATATATTCATAAATATGGAATTGTTAATCCAATAAACTTGAAAGATAAAGATAAGAAATATTTTGAAATTAATGGTGCAGTTGAAAAACCTAAGGCTGATAAAGCACCATCAAATAAAGCTATACTTGGACGTTATGTCTTTAATCCAGAAATTTTAGATATCTTATCTAGAATTGAATATGATGGCAAAAATGAAATTCAAGCAGTTGATGCATTTGATGAATTAATGAAAAAATATAAACAAAAAATTTATGCCTATGAATTTGAAGGCATCAGATATGATCTTGGATCAATGGAAGGCTTTGTTAAAGCAAACATTGATTATGCTTTAGAAGAACCTGAAATTGCAGACAAGATTAAAAGTTTTATTAATGAAAAAGTTAAAGAATTTTAA", "aa": "MTKIIHKELVPILDTPVIDRLVNEALDSGIEEILIVLSERKKDLLKFFSVDQDLQTELIIKGKKDILEKVKRTNRSSCITRVIQKEQFGLGHALASCKEYIDNEPFAIILGDDLIKSKTPAIKQLIEFYNKTGANVIGVQSVAKEYIHKYGIVNPINLKDKDKKYFEINGAVEKPKADKAPSNKAILGRYVFNPEILDILSRIEYDGKNEIQAVDAFDELMKKYKQKIYAYEFEGIRYDLGSMEGFVKANIDYALEEPEIADKIKSFINEKVKEF", "aa_hexdigest": "35ea3657a8e612087c2d43f66493c65d", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_C4XDW2", "query_cov": 1.0, "subject_cov": 0.9105960264900662, "identity": 1.0, "score": 533.0, "evalue": 7.68e-191, "valid": true, "product": "UTP--glucose-1-phosphate uridylyltransferase", "uniref50_id": "UniRef50_W5UT80" }, "pscc": { "uniref50_id": "UniRef50_W5UT80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W5UT80" ], "product": "UTP--glucose-1-phosphate uridylyltransferase" }, "genes": [], "id": "OAIKOHHJFB_179", "locus": "GCA000209735_00137" }, { "type": "tRNA", "sequence": "contig_1", "start": 73193, "stop": 73268, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(tcg)", "amino_acid": "Arg", "anti_codon": "tcg", "score": 65.7, "nt": "GATCTTATAGCTCAATGGACAGAGTGTTGGGTTTCGGCCCCAAAGGTTAAGGGTTCGAGTCCCTTTAAGATCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 73233, 73235 ], "id": "OAIKOHHJFB_35", "locus": "GCA000209735_00138" }, { "type": "cds", "sequence": "contig_1", "start": 73940, "stop": 74095, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCAGATATATTTTTGGATTTATTAAAGTTATATCACCTGCAAAAAAATTAGTTGGTTCATTATTTTCATTGAATAAATTTTTGCTATTTTTAAAATTTGTCGCATATGCTTTTCTTGCATTTTTATCTCATTCTGATGCGAAAACGCGTTGA", "aa": "MIRYIFGFIKVISPAKKLVGSLFSLNKFLLFLKFVAYAFLAFLSHSDAKTR", "aa_hexdigest": "48ea6dff18e84ddbb1ae43ed7d0f4d75", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5859.0645, "isoelectric_point": 10.461107444763183 }, "id": "OAIKOHHJFB_180", "locus": "GCA000209735_00139" }, { "type": "tRNA", "sequence": "contig_1", "start": 74287, "stop": 74362, "strand": "-", "gene": "trnF", "product": "tRNA-Phe(gaa)", "amino_acid": "Phe", "anti_codon": "gaa", "score": 79.1, "nt": "GGCTCTGTAGCTCAGTTGGTAGAGCAACGGACTGAAGCTCCGTGTGTCGCTGGTTCGATTCCTGCCGGAGCCACCA", "db_xrefs": [ "SO:0000267" ], "anti_codon_pos": [ 74327, 74329 ], "id": "OAIKOHHJFB_34", "locus": "GCA000209735_00140" }, { "type": "tRNA", "sequence": "contig_1", "start": 74367, "stop": 74443, "strand": "-", "gene": "trnD", "product": "tRNA-Asp(gtc)", "amino_acid": "Asp", "anti_codon": "gtc", "score": 75.8, "nt": "GGTCCAGTGGTAAAGTGGTTTAATACGTCTCCCTGTCACGGAGAAGATCGCGGGTTCAATTCCCGTCTGGACCGCCA", "db_xrefs": [ "SO:0000256" ], "anti_codon_pos": [ 74440, 74442 ], "id": "OAIKOHHJFB_33", "locus": "GCA000209735_00141" }, { "type": "tRNA", "sequence": "contig_1", "start": 74447, "stop": 74523, "strand": "-", "gene": "trnfM", "product": "tRNA-fMet(cat)", "amino_acid": "fMet", "anti_codon": "cat", "score": 72.3, "nt": "CGCGGAGTGGAGAAGTTTGGTATCTCGCGAGGCTCATAACCTCGAGGCCGCTGGTTCGAGTCCAGCCTCCGCCACCA", "db_xrefs": [ "SO:0000266" ], "id": "OAIKOHHJFB_32", "locus": "GCA000209735_00142" }, { "type": "tRNA", "sequence": "contig_1", "start": 74563, "stop": 74655, "strand": "-", "gene": "trnS", "product": "tRNA-Ser(tga)", "amino_acid": "Ser", "anti_codon": "tga", "score": 76.1, "nt": "GGGTGATTACCCAAGCCTGGCTGAAGGGACTAGTCTTGAAAACTAGCAGGGGCTTCACGGCCCGCGGGGGTTCGAATCCCTCATCACCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 74650, 74652 ], "id": "OAIKOHHJFB_31", "locus": "GCA000209735_00143" }, { "type": "tRNA", "sequence": "contig_1", "start": 74657, "stop": 74731, "strand": "-", "gene": "trnI", "product": "tRNA-Ile2(cat)", "amino_acid": "Ile2", "anti_codon": "cat", "score": 82.6, "nt": "GGACCTATAGCTCAACGGCAGAGCAACCGGCTCATAACCGGTTGGTTACTGGTTCGAATCCAGTTGGGTCCACCA", "db_xrefs": [ "SO:0000263" ], "id": "OAIKOHHJFB_30", "locus": "GCA000209735_00144" }, { "type": "tRNA", "sequence": "contig_1", "start": 74759, "stop": 74835, "strand": "-", "gene": "trnM", "product": "tRNA-Met(cat)", "amino_acid": "Met", "anti_codon": "cat", "score": 78.8, "nt": "GGCGTTGTAGCTCAGTTGGTTAGAGCATCCGGTTCATACCCGGAAGGTCATGAGTTCGACTCTCATCGACGCTACCA", "db_xrefs": [ "SO:0000266" ], "anti_codon_pos": [ 74808, 74810 ], "id": "OAIKOHHJFB_29", "locus": "GCA000209735_00145" }, { "type": "tRNA", "sequence": "contig_1", "start": 74841, "stop": 74917, "strand": "-", "gene": "trnA", "product": "tRNA-Ala(tgc)", "amino_acid": "Ala", "anti_codon": "tgc", "score": 83.0, "nt": "GGGGGATTAGCTCATTTGGCTAGAGCACTTGCCTTGCACGCAAGGGGTGGTGGGTTCGAATCCCATATCCTCCACCA", "db_xrefs": [ "SO:0000254" ], "anti_codon_pos": [ 74886, 74888 ], "id": "OAIKOHHJFB_28", "locus": "GCA000209735_00146" }, { "type": "tRNA", "sequence": "contig_1", "start": 74921, "stop": 74997, "strand": "-", "gene": "trnP", "product": "tRNA-Pro(tgg)", "amino_acid": "Pro", "anti_codon": "tgg", "score": 81.9, "nt": "GGAGAAGTAGCTCAGCTTGGCAGAGCGCTACGTTTGGGACGTAGTGGTCGCAGGTTCAAATCCTGTCTTCTTCACCA", "db_xrefs": [ "SO:0000268" ], "anti_codon_pos": [ 74978, 74980 ], "id": "OAIKOHHJFB_27", "locus": "GCA000209735_00147" }, { "type": "cds", "sequence": "contig_1", "start": 75303, "stop": 75539, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAAAGATAAAATCTGATTTATTAAACAAAATTAAAAACATTCATCGAGAAGAATTTTTATATTTAAATTCAAAAATACTTGATTCATATGCTAAGGGAAAATTAATAAGTTTTTACGATGAAACAGATAAAAACAATTTCTTTATTTTTGAAATCGTTGAAATACAAAAAGTTAATTTTGCAGATATTAAAGAACCTTATATTATTAATACAAGTAAAAGAGATCATTGA", "aa": "MKLKIKSDLLNKIKNIHREEFLYLNSKILDSYAKGKLISFYDETDKNNFFIFEIVEIQKVNFADIKEPYIINTSKRDH", "aa_hexdigest": "d5e72f9b9cdf92903d1042cffd5882ee", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9370.73569999999, "isoelectric_point": 8.831411170959473 }, "id": "OAIKOHHJFB_181", "locus": "GCA000209735_00148" }, { "type": "cds", "sequence": "contig_1", "start": 75579, "stop": 76343, "strand": "+", "frame": 3, "gene": null, "product": "Sucrose-6F-phosphate phosphohydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8L7N0", "UniRef:UniRef90_C4XDW6" ], "nt": "ATGGTTGATAACTTTGTATTTGATATGGATGGTACTCTTTTAAATGAAAATAAAGCTATTTTAAAAGAAAATATTGACGCATTAAAAGAACTTAAGAAAATGGGCAAAAATATTATTGTAGCAACTGGACGTCCATTGTATACATTGCATCATATTGAAGAAGTTCCAACTGAGTTTCCAATTATTTGTTCTAATGGAGCAATTATATATAACAAAGACCATAGTTTATTAAAAGCATATGAAATAGATAAGGAAACAGTTAAAAGTATTTACAAATATTTAAAAAGTCAAGATATGGAATTCCTAATGTATTCGCCTAATTATGTTTTAGGTTGTGAAAATAGTAATTGCAAATATCTTGAAATGCGTAATTATGCTGCAAGTGTTGGTGAAAATTATAAACAAGGTGAGTTCTTTAATGAATTAGAAAAATATAGAATTTGTAAGTTCTTAATACATATACCTTGTACAACAGAAGAAAAGTTAGCAAATTTAAAAGAATATTTAAAACAATTTGATGTTTATTTAGTAAGATCACAATCGACTCTTGCTGATATTATGAGAACAGATGCAACTAAAGGTGAAGCAGTTAAAAATGTTGCTCAAAAATATAATCTTGATTTAAATCGAACAATTTGTTTTGGCGATGCAGAAAATGATACAAGTATGTTCTCAATCTCTAAATACTCAGCTAGTCCTTCAAGTGGAAATATTAAAGCTAAAAATAGATCTTTATTACGTGTTAGAAATCATCAAGTGGCTTGA", "aa": "MVDNFVFDMDGTLLNENKAILKENIDALKELKKMGKNIIVATGRPLYTLHHIEEVPTEFPIICSNGAIIYNKDHSLLKAYEIDKETVKSIYKYLKSQDMEFLMYSPNYVLGCENSNCKYLEMRNYAASVGENYKQGEFFNELEKYRICKFLIHIPCTTEEKLANLKEYLKQFDVYLVRSQSTLADIMRTDATKGEAVKNVAQKYNLDLNRTICFGDAENDTSMFSISKYSASPSSGNIKAKNRSLLRVRNHQVA", "aa_hexdigest": "951c9adc58108e834de067f91d4e433b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 75303, "stop": 76376, "observations": { "mutation": [ 75537, 75564, 76341 ], "stop": [ 75537, 75564, 76341 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDW6", "query_cov": 1.0, "subject_cov": 0.711484593837535, "identity": 1.0, "score": 508.0, "evalue": 2.75e-180, "gene_start": 1, "gene_end": 254, "reference_start": 93, "reference_end": 346, "reference_sequence": "MKLKIKSDLLNKIKNIHREEFLYLNSKILDSYAKGKLISFYDETDKNNFFIFEIVEIQKVNFADIKEPYIINTSKRDHWYKLRIKKDWFDLSMVDNFVFDMDGTLLNENKAILKENIDALKELKKMGKNIIVATGRPLYTLHHIEEVPTEFPIICSNGAIIYNKDHSLLKAYEIDKETVKSIYKYLKSQDMEFLMYSPNYVLGCENSNCKYLEMRNYAASVGENYKQGEFFNELEKYRICKFLIHIPCTTEEKLANLKEYLKQFDVYLVRSQSTLADIMRTDATKGEAVKNVAQKYNLDLNRTICFGDAENDTSMFSISKYSASPSSGNIKAKNRSLLRVRNHQVAWLKEFVEQFKD" }, "paralog": false, "identity": 0.9915966386554622, "score": 704.0, "evalue": 5.15e-260, "description": "Internal stop codon at 75537, 75564, 76341. Nonsense mutation around 75537, 75564, 76341.", "psc": { "uniref90_id": "UniRef90_C4XDW6", "product": "Sucrose-6F-phosphate phosphohydrolase", "uniref50_id": "UniRef50_A0A3P8L7N0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDW6", "UniRef:UniRef50_A0A3P8L7N0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8L7N0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8L7N0" ], "product": "Haloacid dehalogenase-like hydrolase" } }, "id": "OAIKOHHJFB_182", "locus": "GCA000209735_00149" }, { "type": "rRNA", "sequence": "contig_1", "start": 76365, "stop": 77757, "strand": "-", "gene": "rrs", "product": "16S ribosomal RNA", "coverage": 0.908675799086758, "score": 302.9, "evalue": 1.4e-12, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00177", "KEGG:K01977", "SO:0001000" ], "nt": "TTTTTCGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCTGTGTGCCTAATACATGCATGTCGAGCGAAGGTAGCAATACCTTAGCGGCGAATGGGTGAGTAACACGTGCTCAACGTACCCTTCAGTTTGGCATAGCGACTGGAAACAGTCGATAATTTCAAATACTCGTAGTTTTCGCATGAAGATTACGGAAAAGAAGCGTTTGCTTCGCTGGAGGAGCGGGGTGCGTAACATTAGCTAGTTGGTGAGGTAACGGCCCACCAAGGCGATGATGTTTAGCGGGGTTGAGAGACTGAACCGCCACACTGGGACTGAGATACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATATTCCACAATGGGCGAAAGCCTGATGGAGCGACACAGCGTGAAGGATGAAGGTCCTATGGATTGTAAACTTCTGTGGTAAGGGAAGAAAAGACAGAATAGGAAATGATTTTGTTTTGACGGTACCTTATTAGAAGCAACGGCTAACTATGTGCAGCAGCCGCGGTATACATAGGTTGAAGCGTTATCGGAATATTGGCGTAAGAGTCTGTAGTGTTGNTAGTCGGCGTTAATTTGGGCTCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGCAGGTGCGAAAGCAGGACTTAGTGATCCGGCTGTACATTGTGGAATGGCAGTCGCTCAACGGATAAAAGTTACCCTGGGGATAACAGGCTTATCTTGCCCAAGAGATCACATCGACGGCAAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGGAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAAAACGTCGTGAGACAGTTTGGTCCCTATCTGATGTGGGCGTTGGAATATTGATGAGAGCCGCTCTTAGTACGAGAGGACCGGAGTGGACGTACCACTGGTGTTCCAGTTGTTCCGCCAGGAGCATAGCTGGGTAGCTAAGTACGGAAAGGATAACCGCTGAAAGCATCTAAGTGGGAAGCCTCCTCAGAGATAAGTATTCCCTTGAGATTCCTTATAGACTATGAGGTTGATAGGTTGGAGGTGTAAGCGTAGTAATACGTTGAGCTGACCAATACTAATAAATCGATCGGTTTAAAAGTAGAAGTGTATTTATGACATTTCAATGAATTATTGTTACTATTCAGTTTTCAGAGAACATGTGTCACAAGTGTGACATTTTTTATTTCATTTTTTTACCAAAAAGCCTATAAAACAATTAAAAAAGTAAAAAAATTAAAATAGTTTTAATACTATTTTAATTAGTCTTTGAA", "id": "OAIKOHHJFB_39", "locus": "GCA000209735_00150" }, { "type": "gap", "sequence": "contig_1", "start": 77059, "stop": 77158, "strand": ".", "length": 100, "id": "OAIKOHHJFB_1792" }, { "type": "gap", "sequence": "contig_1", "start": 77183, "stop": 77183, "strand": ".", "length": 1, "id": "OAIKOHHJFB_1793" }, { "type": "cds", "sequence": "contig_1", "start": 78008, "stop": 79141, "strand": "-", "frame": 1, "gene": null, "product": "putative tRNA sulfurtransferase", "db_xrefs": [ "RefSeq:WP_015510682.1", "SO:0001217", "UniParc:UPI0001A8C330", "UniRef:UniRef100_C4XDW7", "UniRef:UniRef50_B3PLZ2", "UniRef:UniRef90_C4XDW7" ], "nt": "ATGTACAATAAGATTTTAATTAGATATGGTGAATTGACTCTAAAAGGCAAAAATAGAGTTAATTTTATTGATACTTTAGCTTCAAATATTCGTCATAAAACTAAAGATAAATTAGAGGTTGAATTTGACCGTATGTTTTTGCCTTATAGTCAAGAAAATTTAGATGCATTGCAATACATTTTTGGTATTACTTCTTTCTCACCTGTTATTATTTGTGCTAATGATATTGAAGAAATTAAAAAGCAAGCTTTAAATTTAGCAAAAAATAAAAATGGAACTTTTAAAGTTGCTGCTCGTAGAAACAATAAAAGTTTTCAATACCAATCAAGTCAAATTAACAATATAGTTGGCGGTTTTTTATTAGAAAACAACCAAAATTTAAAAGTTGATGTTAATAATCCAGACTTTATTGTTTATATTGAAGTTAGAGGCAAATTTACTTATGTTTTTAGTGATTTTGTCCAAGCTTTAGGCGGCCTCCCTGTTGGTGTTAGTGGGCAAGTATTGCACTTAATGAGTGGTGGAATTGATAGTCCTGTTGCTGCTTTTCAACTTATGAAAAGAGGTCTTAAAGTTAATTTCTTATCTTTTATTACACCTCCTCAAACTGATCAAGCAACAGTAAATAAAATGAAAGAATTAATCAAAATATTAACTAAATATCAAAACACAAGCACTTTTTATTTAGCTAACTATTCTACTTTAATGAACTACATAGCTTTAGTTTCAAATCAAGCTTATAAAATTACTCTTATGCGTCGGAGCTTTTATCGTATTGCTGCTAAATTAGCTCAAAGTAAAAAAAATCTAGCCATTTCTAATGGCGAAAACTTAGGTCAAGTAGCTTCACAAACTTTAGAATCACTTTATACAATTAGTCATGATATTAACATGCCAATTTTGCGGCCACTATTAACTAATGACAAAATTGAAACAATCAATTTAGCTAAAAAAATTCAAACCTATGATCTTTCAATTACTAAATGTAATGAAACATGTGAGCTTTTTGCTCCTAAAGAACCAGTAACTAAACCTAATGTTCAAGAAGCTCTTAAATTAGAAGAAGAATTAAACATGATTAATGACTTAGAAAAAGAATGCTTAGAAAAAAATATTGAAATAATCAAATTCTAA", "aa": "MYNKILIRYGELTLKGKNRVNFIDTLASNIRHKTKDKLEVEFDRMFLPYSQENLDALQYIFGITSFSPVIICANDIEEIKKQALNLAKNKNGTFKVAARRNNKSFQYQSSQINNIVGGFLLENNQNLKVDVNNPDFIVYIEVRGKFTYVFSDFVQALGGLPVGVSGQVLHLMSGGIDSPVAAFQLMKRGLKVNFLSFITPPQTDQATVNKMKELIKILTKYQNTSTFYLANYSTLMNYIALVSNQAYKITLMRRSFYRIAAKLAQSKKNLAISNGENLGQVASQTLESLYTISHDINMPILRPLLTNDKIETINLAKKIQTYDLSITKCNETCELFAPKEPVTKPNVQEALKLEEELNMINDLEKECLEKNIEIIKF", "aa_hexdigest": "c4f07ca10f714877b3ce2eed61040d2a", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001A8C330", "ncbi_nrp_id": "WP_015510682.1", "uniref100_id": "UniRef100_C4XDW7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C330", "RefSeq:WP_015510682.1", "UniRef:UniRef100_C4XDW7" ] }, "ips": { "uniref100_id": "UniRef100_C4XDW7", "uniref90_id": "UniRef90_C4XDW7" }, "psc": { "uniref90_id": "UniRef90_C4XDW7", "product": "Probable tRNA sulfurtransferase", "uniref50_id": "UniRef50_B3PLZ2" }, "pscc": { "uniref50_id": "UniRef50_B3PLZ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PLZ2" ], "product": "Probable tRNA sulfurtransferase" }, "genes": [], "id": "OAIKOHHJFB_183", "locus": "GCA000209735_00151" }, { "type": "cds", "sequence": "contig_1", "start": 79131, "stop": 79280, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAAAAGCATGCATTATCGATTTAATAATCCAGACTTTAATGAACTCTTAATTTCAGTCAATAAACACATGCGAGCTAAGAAATTTGATGAAGCTTTCTCACTACTAGCAACCTGCATGAAAAAAGGAAAAAAATATGTACAATAA", "aa": "MLKSMHYRFNNPDFNELLISVNKHMRAKKFDEAFSLLATCMKKGKKYVQ", "aa_hexdigest": "e844a480200caf064afe2d9ccdfffa01", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5852.8982000000005, "isoelectric_point": 9.844273948669432 }, "id": "OAIKOHHJFB_184", "locus": "GCA000209735_00152" }, { "type": "cds", "sequence": "contig_1", "start": 79509, "stop": 79913, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAGAAAGAATCAAAAAAATCTATGCTGATTATTATACTTTAAACAAGAAAACTGCTCTTAATGCTGAGTTTCAAGATTTCATAAATAATGAATTAGAAAACATAGGCAAAATGATTAATGAGCTTGATCAAAAATTAAGCTATATTTCAATTGATTTAGATGATTTTGACAGCAATTTTGTAACCAAGATTTCACAAGAATTGTTACAACTTAGAAACCAATATGATAGCTTTAATAAAGCTAATAAAGCTAATGGCAATGTTAGTTTAATGGAGATGCAAACACTACTTCATGGCATAATGGAATTAATCAAAAAATGCAATGATAAAATTGAGATTTTTAACCTCGATACTTATCAAAAAAATTACAATGCTTACTACTTAAAAATTTTGGAAACTTGA", "aa": "MQERIKKIYADYYTLNKKTALNAEFQDFINNELENIGKMINELDQKLSYISIDLDDFDSNFVTKISQELLQLRNQYDSFNKANKANGNVSLMEMQTLLHGIMELIKKCNDKIEIFNLDTYQKNYNAYYLKILET", "aa_hexdigest": "9c40a421b6c921aefbe53378678d0e5e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15871.892799999992, "isoelectric_point": 5.021918296813964 }, "id": "OAIKOHHJFB_185", "locus": "GCA000209735_00153" }, { "type": "cds", "sequence": "contig_1", "start": 80307, "stop": 80732, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGTAATTAAAATTATTGTACCAATAGTTATTTTGGCCTGTTTAGGTCTTTTGGGCATTTTTATTTTCCTTTATATTAGAAAAACTAATCTTAATAAATACATTAAATTTTTAGATGATTCAACTAAAAATATTAAAAATAATCTAACAGGACGTAACGCAACAATTCAACGTTTTATTACTTTATCAAATACTCAAGAAACATATAAAGCTGCTTTAAATCAATTAAAAAGTTTAGACAACAAATTAAAAAAAATAATTGTTGATTTAAATGATAAATATAATGCATTAAGAAAAGCGGCTAAAGCTTATAAACTTAAAGAAGCTAAAAAACTTTACTTAGAAATTTTGCCTAAATATGAAGAATGCATTTCAATGCATAAACAATTTGATGAAAAAACTAAGAACTTAAATAAACATTGA", "aa": "MQVIKIIVPIVILACLGLLGIFIFLYIRKTNLNKYIKFLDDSTKNIKNNLTGRNATIQRFITLSNTQETYKAALNQLKSLDNKLKKIIVDLNDKYNALRKAAKAYKLKEAKKLYLEILPKYEECISMHKQFDEKTKNLNKH", "aa_hexdigest": "160bce9d077f1db600af8b17f19b07a8", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16433.37039999999, "isoelectric_point": 9.837633705139158 }, "id": "OAIKOHHJFB_186", "locus": "GCA000209735_00154" }, { "type": "cds", "sequence": "contig_1", "start": 80836, "stop": 81036, "strand": "-", "frame": 2, "gene": "rpmF", "product": "50S ribosomal protein L32", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015934", "RefSeq:WP_013354850.1", "SO:0001217", "UniParc:UPI0001E32F3D", "UniRef:UniRef100_A0AB32XC94", "UniRef:UniRef50_Q4A6N8", "UniRef:UniRef90_C4XDW9" ], "nt": "GTGGCTATAGTTCCAAAACGTAAGACATCTAAACAACGTAAACACAAAAGACGTACCCATGATGCTTTACCTGTTCAAAATCTTGTTGCATGCAAAAACTGTTCTCAATTAATTCAACAACACCGTGTATGTGAATCTTGTGGATTTTATAAAGGTAAAAAAGTTGAAGGTTACAAAAGTTTAGATATGCGTAATGCTTAA", "aa": "MAIVPKRKTSKQRKHKRRTHDALPVQNLVACKNCSQLIQQHRVCESCGFYKGKKVEGYKSLDMRNA", "aa_hexdigest": "ed8e2872a40e75b33585b60cb4069913", "start_type": "GTG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32F3D", "ncbi_nrp_id": "WP_013354850.1", "uniref100_id": "UniRef100_A0AB32XC94", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F3D", "RefSeq:WP_013354850.1", "UniRef:UniRef100_A0AB32XC94" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XC94", "uniref90_id": "UniRef90_C4XDW9" }, "psc": { "uniref90_id": "UniRef90_C4XDW9", "gene": "rpmF", "product": "50S ribosomal protein L32", "uniref50_id": "UniRef50_Q4A6N8", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A6N8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6N8" ], "product": "Large ribosomal subunit protein bL32" }, "genes": [ "rpmF" ], "id": "OAIKOHHJFB_187", "locus": "GCA000209735_00155" }, { "type": "cds", "sequence": "contig_1", "start": 81199, "stop": 81945, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATATTAATGAACAACTAAAGAGTGCAAATTATCAAATTTATCAAAATGAAATGAAGCAAATTTTAGATGATTTTTATAGCAAAAATGGAGATTATAAAAATCAAAAAATTCAATTTACATTACCTATTTTTGCTGAAAAGAGTTCATTTATTGACAGTTTATATAAAAGAATAGCCTTCTTGTACAACAACCTAGACAATCGTTTAAATGTTAATTTTGAATATGCTTCAAAAGAAGCAAATAATTACTTTTCTAAATATCAAAACTTTAGTGCAGTTGATAATTCATTTAGTAACTATTTAATTAATTTAATAACAGCAAATAACCAATTCTTTTTAAATTTATTCTTATATTCAAAAGTCAATTTAGAATCAATTAACTATGATAAAAATATCAAAAAAATTATTTCAACAATAGAAGAAACAATTAATAAAGTTGATTACTTTAAAAATTATGAGTTGCAAAAAATTAAAGCAAACAGTTCACTTTTAGATCAAATTTTGCAAAAAAATAATTTGAAATTGAAAGAATTCAAAGCTAGATTTAAAGAAAAAATTCAAGCTTTAAATTTTGATCAACAATTCAATATTTTAAAAAGTATTGACTCATTTTTAGGAATCAGAGTAACTGAAGATAATTTCCTTTTTACTAACGAATACCAAAAATCAATAGTTCAATACTTCTACACAAAACCAATAAATCTAGATGGTTTTACTTATTTTCAAGATATTACTTTTAACTAA", "aa": "MDINEQLKSANYQIYQNEMKQILDDFYSKNGDYKNQKIQFTLPIFAEKSSFIDSLYKRIAFLYNNLDNRLNVNFEYASKEANNYFSKYQNFSAVDNSFSNYLINLITANNQFFLNLFLYSKVNLESINYDKNIKKIISTIEETINKVDYFKNYELQKIKANSSLLDQILQKNNLKLKEFKARFKEKIQALNFDQQFNILKSIDSFLGIRVTEDNFLFTNEYQKSIVQYFYTKPINLDGFTYFQDITFN", "aa_hexdigest": "b6e3b16ac68c30c558a6730a4e6cb1bc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29635.134500000044, "isoelectric_point": 8.384322929382325 }, "id": "OAIKOHHJFB_188", "locus": "GCA000209735_00156" }, { "type": "cds", "sequence": "contig_1", "start": 82150, "stop": 83229, "strand": "-", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX0", "UniRef:UniRef90_C4XDX0" ], "nt": "GTGACTCTGAAGGATAATAGTCAAAAAATTTTTAATAGTGATGATGTTAAAGAAAAAGTTGATCAAGCTCTTCAAAGTAGTACTAAAGTTAATGAAGATGGAGCATTAAGCGGTATTAAACAAATTTATTCTGATGAAAATAATAATATAAATTCTAAAGAATTTTTTGAAGCTTTAAATAGTGCAACTAAAATTGAATTTGAATTAAAAGACGATGTTTATTATTCAGATTATAAAGGCAAAAAAACAAATCATAAATTACAAGCCCAAGACTATTTAACTAGCTTTAATTTGAAACAAAATCGTGAACAATTTTCAGCTCTTTTAGACAACTATTCTTTGAAAAATTTAGGCCAAAATAGCATCCAAAATAACAAGTTAATTTTTGAATTAAACGATGCTCAAAAAAGCAAAAATAATAACTTTATTAAACTATTTACAACTCAAGAAATTACTAATAATTTGATCTTCAATCCAATATCAACAGACTACTATAAAAATAATAAAAATAGTTATGGCAAAAATTACCAAAACATCTGTTTTATAGGTTATTATGTTTTAAATAAGAACACTGTTGATGAGCAAATTTTTATTAAAAATAATAACTCTGCTTTAGATAGTTTTAACCAAAATGAAAAGCAAAATATTCTTAATAAAGTGACTATAAAATATAATCCAATTCAAAACGATGAGCCTACCTTTAGATTACAAAGTTATAATGCATTTAAACAAAATCTTTTAAGTGAAGCTTCTTATGATATTTTTAACCAATTGCAAAAAGATGAAATAGATAATTTCTCTAATCTTTTTGGTGTTACTTATACATTATTTAAACCAAATAATAGTAATGTTATAAGCTATTTTTACAATGTTAATCCATATGCTAAAAATAACAAAGATTTCAAATTTAATGAAGCTTTTAGCTTACTATTTTATAATCAGAAATTAAATGAATTAAAAGATCAAAATTTAGTAAATAAAAAATATTATGAACCACATCAAATTGTGTTCAGAAATATTTTAAATAACTGTATTAATCAATATTCATTTATTAAAAATTTGAATCATAATACTTACTGA", "aa": "MTLKDNSQKIFNSDDVKEKVDQALQSSTKVNEDGALSGIKQIYSDENNNINSKEFFEALNSATKIEFELKDDVYYSDYKGKKTNHKLQAQDYLTSFNLKQNREQFSALLDNYSLKNLGQNSIQNNKLIFELNDAQKSKNNNFIKLFTTQEITNNLIFNPISTDYYKNNKNSYGKNYQNICFIGYYVLNKNTVDEQIFIKNNNSALDSFNQNEKQNILNKVTIKYNPIQNDEPTFRLQSYNAFKQNLLSEASYDIFNQLQKDEIDNFSNLFGVTYTLFKPNNSNVISYFYNVNPYAKNNKDFKFNEAFSLLFYNQKLNELKDQNLVNKKYYEPHQIVFRNILNNCINQYSFIKNLNHNTY", "aa_hexdigest": "736004efb93a7cc0682ce9e6cea033de", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 81847, "stop": 83529, "observations": { "mutation": [ 82152, 83328 ], "stop": [ 82152, 83328 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDX0", "query_cov": 1.0, "subject_cov": 0.45790816326530615, "identity": 0.997, "score": 688.0, "evalue": 3.88e-243, "gene_start": 1, "gene_end": 359, "reference_start": 109, "reference_end": 467, "reference_sequence": "MKRKIKKYGSLFLISATLTSSVATLSCNNIRQVNPKDSYIKKYNEPNKIKDNFIINKVKRIDNDFESLLTAPLIRWKSLGKSKFDHLNKFFYKTTTRFLEFYLAKSITVTLKDNSQKIFNSDDVKEKVDQALQSSTKVNEDGALSGIKQIYSDENNNINSKEFFEALNSATKIEFELKDDVYYSDYKGKKTNHKLQAQDYLTSFNLKQNREQFSALLDNYSLKNLGQNSIQNNKLIFELNDAQKSKNNNFIKLFTTQEITNNLIFNPISTDYYKNNKNSYGKNYQNICFIGYYVLNKNTVDEQIFIKNNNSALDSFNQNEKQNILNKVTIKYNPIQNDEPTFRLQSYNAFKQNLLSEASYDIFNQLQKDEIDNFSNLFGVTYTLFKPNNSNVISYFYNVNPYAKNNKDFKFNEAFSLLFYNQKLNELKDQNLVNKKYYEPHQIVFRNILNNCINQYSFIKNLNHNTYWNSFASQSLNYDSSNNSGILDNQNVNFLDDINRVYDYHFDYKTNKFDNKIIDFNGSDGLIKHSINSKNILDINEQLKSANYQIYQNEMKQILDDFYSKNGDYKNQKIQFTLPIFAEKSSFIDSLYKRIAFLYNNLDNRLNVNFEYASKEANNYFSKYQNFSAVDNSFSNYLINLITANNQFFLNLFLYSKVNLESINYDKNIKKIISTIEETINKVDYFKNYELQKIKANSSLLDQILQKNNLKLKEFKARFKEKIQALNFDQQFNILKSIDSFLGIRVTEDNFLFTNEYQKSIVQYFYTKPINLDGFTYFQDITFN" }, "paralog": false, "identity": 0.9964285714285714, "score": 1123.0, "evalue": 0.0, "description": "Internal stop codon at 82152, 83328. Nonsense mutation around 82152, 83328.", "psc": { "uniref90_id": "UniRef90_C4XDX0", "product": "Lipoprotein", "uniref50_id": "UniRef50_C4XDX0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDX0", "UniRef:UniRef50_C4XDX0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDX0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX0" ], "product": "Lipoprotein" } }, "id": "OAIKOHHJFB_189", "locus": "GCA000209735_00157" }, { "type": "cds", "sequence": "contig_1", "start": 83326, "stop": 83553, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGAAAAATTAAAAAATATGGCAGCTTATTTTTAATTAGTGCAACACTTACTTCTAGTGTCGCTACTCTTTCATGCAACAATATTAGACAAGTTAATCCTAAAGATAGTTATATTAAAAAATATAATGAACCAAACAAAATTAAAGACAATTTTATTATTAACAAAGTTAAAAGAATTGATAATGATTTTGAGAGTTTATTAACTGCACCTTTAATTAGATGA", "aa": "MKRKIKKYGSLFLISATLTSSVATLSCNNIRQVNPKDSYIKKYNEPNKIKDNFIINKVKRIDNDFESLLTAPLIR", "aa_hexdigest": "a39414891b0d2d57b81b953746b23222", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8661.062799999996, "isoelectric_point": 10.00628299713135 }, "id": "OAIKOHHJFB_190", "locus": "GCA000209735_00158" }, { "type": "cds", "sequence": "contig_1", "start": 83562, "stop": 83771, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGCTTTAGCTTCATTAGCTTTCTTTAATATTTATAAAATTGATCAAAGCTTAAATATTGGAAACGTTTTTAAAGATATAGTTGACAATCTAAAAAATGTTGAATATACAATTTTTGTTTCAATATTAGCAAGTTTTTATGTAATATTAGTAAAATTCTTAGGTATAAATTTCTTTATTGCGAGTGTACCTAAATTAGAAAAGTAA", "aa": "MLALASLAFFNIYKIDQSLNIGNVFKDIVDNLKNVEYTIFVSILASFYVILVKFLGINFFIASVPKLEK", "aa_hexdigest": "f37d752acdb7695fd3bab4d7348c5f6d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7835.241599999996, "isoelectric_point": 8.006925010681151 }, "id": "OAIKOHHJFB_191", "locus": "GCA000209735_00159" }, { "type": "cds", "sequence": "contig_1", "start": 83850, "stop": 84710, "strand": "-", "frame": 3, "gene": null, "product": "ABC transmembrane type-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX1", "UniRef:UniRef90_C4XDX1" ], "nt": "ATGGCATTTAATCATGAGCACCACCATTCTCACGAAAAAGCAGGCCAAAGTCATATTGAATTTGAAAAAAGTCTTTTCACTTTAACTCACCATAAACACTTCCAAGTAAATATCAGCAATAACAAAAGTGAATTTAAACAATACTTAATTCGTTTCTTTAGCAAAAAGATCAACATTGCTTTAGTTTCAATTATTGGAATCTTCTTACTGTTTTTGACTTTAAGTGCTATTTTTAGCCCTTATTCGCCAAAAACTAGTGTCATCGATTCACAACTTGCTTATAATTTGCCTAACTACTTAAATGCAAGTATTACTAAAAAGTTTGCACAAAGTGACCCCTTATATCAATTTATTTTGCAAACTCAAAAAGATCATCCTAATTTAAAAATAGTCATAAATGAAACACCTGATTTTGACTTAGTTAATTTAACTTACAATCCATATCAATTGCTTTATGCCTTAAAAGGCACTAACTATATCTTTTTATATGGTACCAATTCTCAAGGTATAGACCGCTTTAGTTTCTTTATTCATTCATTTGGCATAAGTATTTTAGTAACATTTGTGGCTGCTTTAATCCAATTATTTTTAGGGACCTTTTTAGGTTCAATAGTGGCTTATTATTCAAATAAAAGCAGTGCTAAATTTAGTTACTATTTAATTAGTACTATTAATATTGTGCCTTTCCTTATTGTAGTTTTCTTGATTTTTAAAATAAGTTCATATAACCATCTTAATGCTATATTAATTTTAGGTTTTATTGGTTCCCTTAGCTTCTTTTATTCAAGTTATTCAATAGGGCTTGAAATCAAAAACAGTGAATTTATTTTAGCTTACAAAGCTAATGGCTTGAGTAATTGA", "aa": "MAFNHEHHHSHEKAGQSHIEFEKSLFTLTHHKHFQVNISNNKSEFKQYLIRFFSKKINIALVSIIGIFLLFLTLSAIFSPYSPKTSVIDSQLAYNLPNYLNASITKKFAQSDPLYQFILQTQKDHPNLKIVINETPDFDLVNLTYNPYQLLYALKGTNYIFLYGTNSQGIDRFSFFIHSFGISILVTFVAALIQLFLGTFLGSIVAYYSNKSSAKFSYYLISTINIVPFLIVVFLIFKISSYNHLNAILILGFIGSLSFFYSSYSIGLEIKNSEFILAYKANGLSN", "aa_hexdigest": "b20b7d7adee4e54de4dedb54bcbec789", "start_type": "ATG", "rbs_motif": "AGGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 83562, "stop": 84710, "observations": { "mutation": [ 83813, 83852 ], "stop": [ 83813, 83852 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDX1", "query_cov": 1.0, "subject_cov": 0.7486910994764397, "identity": 1.0, "score": 540.0, "evalue": 5.68e-192, "gene_start": 1, "gene_end": 286, "reference_start": 1, "reference_end": 286, "reference_sequence": "MAFNHEHHHSHEKAGQSHIEFEKSLFTLTHHKHFQVNISNNKSEFKQYLIRFFSKKINIALVSIIGIFLLFLTLSAIFSPYSPKTSVIDSQLAYNLPNYLNASITKKFAQSDPLYQFILQTQKDHPNLKIVINETPDFDLVNLTYNPYQLLYALKGTNYIFLYGTNSQGIDRFSFFIHSFGISILVTFVAALIQLFLGTFLGSIVAYYSNKSSAKFSYYLISTINIVPFLIVVFLIFKISSYNHLNAILILGFIGSLSFFYSSYSIGLEIKNSEFILAYKANGLSNWRIIYKIIFIHCLWRNIALVSDSLSLNMLALASLAFFNIYKIDQSLNIGNVFKDIVDNLKNVEYTIFVSILASFYVILVKFLGINFFIASVPKLEK" }, "paralog": false, "identity": 0.9947643979057592, "score": 740.0, "evalue": 1.57e-273, "description": "Internal stop codon at 83813, 83852. Nonsense mutation around 83813, 83852.", "psc": { "uniref90_id": "UniRef90_C4XDX1", "product": "ABC transmembrane type-1 domain-containing protein", "uniref50_id": "UniRef50_C4XDX1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDX1", "UniRef:UniRef50_C4XDX1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDX1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX1" ], "product": "ABC transmembrane type-1 domain-containing protein" } }, "id": "OAIKOHHJFB_192", "locus": "GCA000209735_00160" }, { "type": "cds", "sequence": "contig_1", "start": 84700, "stop": 85332, "strand": "-", "frame": 2, "gene": null, "product": "ABC transmembrane type-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX2", "UniRef:UniRef90_C4XDX2" ], "nt": "ATGACTGATTTAGTCCTCTCATTTATTGTCTTTATTTTAGCTGCTATTCCTACTTTTATCTTTGCACCAATAATGCTAATTATTTCAGAAGTCAATGACTTACCAGTTAATTTTATAGAGCCATCAGCTTTTGGTTTTGGTTATACATTGCTTTCTTTAATACTTCCTATTTCACTTTTATCTTTAGGAATCATTGCATTCTTTGCAACCATTACCAAAAATAATCTTGTTCAAATTCTAAAAAAAGATTATGTAATCACTTTAAAAGCAAATGGCTTAAGTACTTTACAAATATTTAATAAAGCTGTTATTAAAAATTTATTTATTTCATTAGTTAACCAAATAGTGCCAATTTTAGTTTTAACTATAAGTTTTAGCTTAATTATCGAACGTATTTTTCAAATACCAGGTCAAAGTGTTATTTTAATTAGCATGTTTGATCAAAAAGAAATTAATGTTATTATGGCTTTAGTATTTTTTAAAGCTTTGCTTTTATTCTTATTAGCTTTTATTTGTGAATTAACTTATGATTTATTGCAAGTTGAAAATGGTTATAATTTTAATTTCAATTTTAACTTCAAAGAAAATAGAGAAAAAGATAGATTCAGAAAGGAGGCGCATAATGGCATTTAA", "aa": "MTDLVLSFIVFILAAIPTFIFAPIMLIISEVNDLPVNFIEPSAFGFGYTLLSLILPISLLSLGIIAFFATITKNNLVQILKKDYVITLKANGLSTLQIFNKAVIKNLFISLVNQIVPILVLTISFSLIIERIFQIPGQSVILISMFDQKEINVIMALVFFKALLLFLLAFICELTYDLLQVENGYNFNFNFNFKENREKDRFRKEAHNGI", "aa_hexdigest": "13cfeb48ab48407962d2b587c9679db2", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 84700, "stop": 85632, "observations": { "mutation": [ 85356 ], "stop": [ 85356 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDX2", "query_cov": 1.0, "subject_cov": 0.6306306306306306, "identity": 1.0, "score": 354.0, "evalue": 1.05e-120, "gene_start": 1, "gene_end": 210, "reference_start": 124, "reference_end": 333, "reference_sequence": "MKRFFYVERWIFMNFSKYFFKKILLTFLGIFITVTLGYFVIALFINNSQINREPIIKNYFTFLGSIFSGFGKPFSTNNSGFNSSLELFFHYYKFSLLFVSISFVFSFFIGVFVGIWLGYKNNKMTDLVLSFIVFILAAIPTFIFAPIMLIISEVNDLPVNFIEPSAFGFGYTLLSLILPISLLSLGIIAFFATITKNNLVQILKKDYVITLKANGLSTLQIFNKAVIKNLFISLVNQIVPILVLTISFSLIIERIFQIPGQSVILISMFDQKEINVIMALVFFKALLLFLLAFICELTYDLLQVENGYNFNFNFNFKENREKDRFRKEAHNGI" }, "paralog": false, "identity": 0.9870967741935484, "score": 570.0, "evalue": 3.72e-208, "description": "Internal stop codon at 85356. Nonsense mutation around 85356.", "psc": { "uniref90_id": "UniRef90_C4XDX2", "product": "ABC transmembrane type-1 domain-containing protein", "uniref50_id": "UniRef50_C4XDX2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDX2", "UniRef:UniRef50_C4XDX2" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDX2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX2" ], "product": "ABC transmembrane type-1 domain-containing protein" } }, "id": "OAIKOHHJFB_193", "locus": "GCA000209735_00161" }, { "type": "cds", "sequence": "contig_1", "start": 85354, "stop": 85665, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTTAGCAAATACTTTTTCAAAAAAATTCTACTTACATTCTTAGGTATTTTTATAACAGTTACCCTTGGCTATTTTGTTATAGCTTTATTTATTAATAACTCACAAATAAACAGAGAACCAATTATCAAAAATTATTTCACTTTCTTAGGTTCTATTTTTAGTGGTTTTGGTAAACCATTTTCAACTAATAATAGTGGTTTCAATTCATCGTTAGAATTATTCTTCCATTATTATAAATTTAGTTTACTTTTTGTAAGCATTTCTTTTGTATTTAGCTTTTTTATAGGTGTTTTTGTCGGAATATGA", "aa": "MNFSKYFFKKILLTFLGIFITVTLGYFVIALFINNSQINREPIIKNYFTFLGSIFSGFGKPFSTNNSGFNSSLELFFHYYKFSLLFVSISFVFSFFIGVFVGI", "aa_hexdigest": "4782be8f2d41c46e4e81b958565d33d3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11920.856699999988, "isoelectric_point": 9.756339073181149 }, "id": "OAIKOHHJFB_194", "locus": "GCA000209735_00162" }, { "type": "cds", "sequence": "contig_1", "start": 85719, "stop": 85988, "strand": "-", "frame": 3, "gene": null, "product": "Phosphocarrier protein HPr", "db_xrefs": [ "RefSeq:WP_013354854.1", "SO:0001217", "UniParc:UPI0001A8C336", "UniRef:UniRef100_C4XDX3", "UniRef:UniRef50_A0A1H9FWZ8", "UniRef:UniRef90_A0A3P8MEF8" ], "nt": "ATGAAAGAATTTACAGCTTCAATTATAGATCCAATTGGTCTTCACGCAAGACCTGCTAGTGTTGTTTCAACAATTGCTTCAAAATTCAAGAGTGATGTGCAAATTGTTTTAGACAAGACAGGAAAAGTTGGAAATCTTAAATCAATTATGAATGTTATGTCTTTAGGTGTTAAAAAAGGCGACGTAATTACTATTAAAATCAATGGAGCAGATGAAGAAGAAGCATACAAAGCTATTTATGATGCTTTTAAATCAAATGAAATTATTTAA", "aa": "MKEFTASIIDPIGLHARPASVVSTIASKFKSDVQIVLDKTGKVGNLKSIMNVMSLGVKKGDVITIKINGADEEEAYKAIYDAFKSNEII", "aa_hexdigest": "3b24c14dcfdb3c64b760de42af70285b", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C336", "ncbi_nrp_id": "WP_013354854.1", "uniref100_id": "UniRef100_C4XDX3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C336", "RefSeq:WP_013354854.1", "UniRef:UniRef100_C4XDX3" ] }, "ips": { "uniref100_id": "UniRef100_C4XDX3", "uniref90_id": "UniRef90_A0A3P8MEF8" }, "psc": { "uniref90_id": "UniRef90_A0A3P8MEF8", "product": "Phosphocarrier protein HPr", "uniref50_id": "UniRef50_A0A1H9FWZ8" }, "pscc": { "uniref50_id": "UniRef50_A0A1H9FWZ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1H9FWZ8" ], "product": "Phosphocarrier protein HPr" }, "genes": [], "id": "OAIKOHHJFB_195", "locus": "GCA000209735_00163" }, { "type": "cds", "sequence": "contig_1", "start": 86191, "stop": 86643, "strand": "-", "frame": 2, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX4", "UniRef:UniRef90_C4XDX4" ], "nt": "TTGATCTTAGATGAGCCTGCAGCAAACTTAGATCCAAAAGTTAGAAATGAACTTTATTTTAACTTAAGCAAACTTCACAAACAAGGCAAAACCATTTTTGTTTCTTCTCATATTTTAAGTGAGCTAGAACAATATATTGATAGTCTAACTGTTTTAGATAAAGGTAAAGTTTTATATTCTGGTGACTTAGAAATTTTAAAAAATTCAAACTTTGAAGTATTTATTAAAACTGAACAAATTAATGAAACTATTAAAGCCTTAGAAAAAGCAAAACTAGTTTCATCAAACAAAGAATATCAATGTCGAGAAACGGGTATTGCTGTTGATTTACCTTTCAATAAAATCACAAACATAACAAAAGCATTAATTAGTAGTAATATCGATTTTGAAAACATTGGAAAAAATATTCAAACCTTAAACGAAAAGTTTTTCAAAGTTGAAAAAGCAGAATAA", "aa": "MILDEPAANLDPKVRNELYFNLSKLHKQGKTIFVSSHILSELEQYIDSLTVLDKGKVLYSGDLEILKNSNFEVFIKTEQINETIKALEKAKLVSSNKEYQCRETGIAVDLPFNKITNITKALISSNIDFENIGKNIQTLNEKFFKVEKAE", "aa_hexdigest": "6b69a40d31f2dd67ac384d48409265bd", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 86191, "stop": 86943, "observations": { "mutation": [ 86727 ], "stop": [ 86727 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDX4", "query_cov": 1.0, "subject_cov": 0.49019607843137253, "identity": 0.993, "score": 288.0, "evalue": 3.69e-96, "gene_start": 1, "gene_end": 150, "reference_start": 157, "reference_end": 306, "reference_sequence": "MSQNAIEFKNVTMSFKNFKALDNVTFSIKKGEFHGFIGANGAGKTTSFRSLLYFYPEVQGEILIDGINFKNKKSRIKIGYVPEVATFPKSLNIYDYLVALAELNGLKNDEAKKRIEELLIELDVNKKIWKKIGKNLSSGQQKKVILVQALIHNPEILILDEPAANLDPKVRNELYFNLSKLHKQGKTIFVSSHILSELEQYIDSLTVLDKGKVLYSGDLEILKNSNFEVFIKTEQINETIKALEKAKLVSSNKEYQCRETGIAVDLPFNKITNITKALISSNIDFENIGKNIQTLNEKFFKVEKAE" }, "paralog": false, "identity": 0.996, "score": 479.0, "evalue": 8e-174, "description": "Internal stop codon at 86727. Nonsense mutation around 86727.", "psc": { "uniref90_id": "UniRef90_C4XDX4", "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_C4XDX4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDX4", "UniRef:UniRef50_C4XDX4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDX4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX4" ], "product": "ABC transporter domain-containing protein" } }, "id": "OAIKOHHJFB_196", "locus": "GCA000209735_00164" }, { "type": "cds", "sequence": "contig_1", "start": 86725, "stop": 87111, "strand": "-", "frame": 2, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX4", "UniRef:UniRef90_C4XDX4" ], "nt": "ATGTCACAAAACGCAATTGAATTTAAAAATGTTACAATGAGTTTTAAAAATTTTAAAGCTTTAGACAATGTGACATTTTCAATCAAAAAAGGTGAATTTCATGGATTTATTGGAGCAAATGGAGCAGGTAAAACTACAAGTTTCCGTTCATTATTGTATTTTTATCCAGAAGTCCAAGGTGAAATTTTAATAGATGGAATTAACTTTAAAAACAAAAAAAGTAGAATCAAAATTGGTTATGTTCCTGAAGTAGCAACATTTCCTAAAAGTCTGAATATTTATGATTACTTAGTTGCTTTAGCTGAATTAAATGGACTTAAAAATGACGAAGCTAAAAAAAGAATTGAAGAATTATTAATCGAGTTAGATGTTAACAAAAAAATATGA", "aa": "MSQNAIEFKNVTMSFKNFKALDNVTFSIKKGEFHGFIGANGAGKTTSFRSLLYFYPEVQGEILIDGINFKNKKSRIKIGYVPEVATFPKSLNIYDYLVALAELNGLKNDEAKKRIEELLIELDVNKKI", "aa_hexdigest": "fefebced50cd257dea0e9a8c79248d32", "start_type": "ATG", "rbs_motif": "AGGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 86422, "stop": 87111, "observations": { "mutation": [ 86727 ], "stop": [ 86727 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDX4", "query_cov": 1.0, "subject_cov": 0.41830065359477125, "identity": 1.0, "score": 252.0, "evalue": 1.98e-82, "gene_start": 1, "gene_end": 128, "reference_start": 1, "reference_end": 128, "reference_sequence": "MSQNAIEFKNVTMSFKNFKALDNVTFSIKKGEFHGFIGANGAGKTTSFRSLLYFYPEVQGEILIDGINFKNKKSRIKIGYVPEVATFPKSLNIYDYLVALAELNGLKNDEAKKRIEELLIELDVNKKIWKKIGKNLSSGQQKKVILVQALIHNPEILILDEPAANLDPKVRNELYFNLSKLHKQGKTIFVSSHILSELEQYIDSLTVLDKGKVLYSGDLEILKNSNFEVFIKTEQINETIKALEKAKLVSSNKEYQCRETGIAVDLPFNKITNITKALISSNIDFENIGKNIQTLNEKFFKVEKAE" }, "paralog": false, "identity": 0.9956331877729258, "score": 444.0, "evalue": 3.41e-160, "description": "Internal stop codon at 86727. Nonsense mutation around 86727.", "psc": { "uniref90_id": "UniRef90_C4XDX4", "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_C4XDX4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDX4", "UniRef:UniRef50_C4XDX4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDX4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDX4" ], "product": "ABC transporter domain-containing protein" } }, "id": "OAIKOHHJFB_197", "locus": "GCA000209735_00165" }, { "type": "cds", "sequence": "contig_1", "start": 87154, "stop": 87624, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGCCATTAATATATAGAAGTACCATTTTTCTCTCAGCTAATGAAGATCCTTCAAAAGCAAAAAATGAAGAAAGAGAATTCAGAAATTTTGTTGAAGATAATTTAACTTTTGACAAAAAAGAAGAATTATTTAAAAAAACTCGTTACTTAAGCTTTGACTATAACTCTATTGCAAAAAAACTTTTCTTTACAATCTTGGTAAAAAATAATTTATATTCTATAGTTGATGCAATGTCAAAAGTTATTATGAATGATTATCGCGAAACTTTAAAAATAAATCAAAATAAAGAATTCTTTAATTCAAGACTTGAAGAAATTTTTGGCAGCATGCTAATTAATAAAGATTTATTAAAAGAAAACAACATTGATTTGAAATTTTACAAAGTACAAAAAGTTAATATATTTCCATCTTACATGCAAATAATTATTTACTTTTTAATGGCTACACCTTTAGCTATTGGCGGATGA", "aa": "MVPLIYRSTIFLSANEDPSKAKNEEREFRNFVEDNLTFDKKEELFKKTRYLSFDYNSIAKKLFFTILVKNNLYSIVDAMSKVIMNDYRETLKINQNKEFFNSRLEEIFGSMLINKDLLKENNIDLKFYKVQKVNIFPSYMQIIIYFLMATPLAIGG", "aa_hexdigest": "9e6cfaac78431b44398c31123c76e476", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18518.32829999999, "isoelectric_point": 8.940878486633302 }, "id": "OAIKOHHJFB_198", "locus": "GCA000209735_00166" }, { "type": "cds", "sequence": "contig_1", "start": 87868, "stop": 88137, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTTTATAATGATTTTATTTTAGATGATCCAAAGAGCAACCGCTTTATTCCAATTATGAGTGAAGGAGTTCATAACCAATATTTCTATAAAAGAGATATGGAATCTGTTTTGAATAATTATGATTATGTCACAAATGGAAATCAAAAGATTTATATTATTGATTATGATTTAAGCACAACAGATCGACTTAATCCATATCAAAAACAAGAAACCTTAGAACAAAAAATTACTTATATGGCTCGCAAAAAACACATTATAAAATGA", "aa": "MNFYNDFILDDPKSNRFIPIMSEGVHNQYFYKRDMESVLNNYDYVTNGNQKIYIIDYDLSTTDRLNPYQKQETLEQKITYMARKKHIIK", "aa_hexdigest": "cc82759af5f94e1ac0d146dbe6ee141e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10841.131899999993, "isoelectric_point": 6.715038108825683 }, "id": "OAIKOHHJFB_199", "locus": "GCA000209735_00167" }, { "type": "cds", "sequence": "contig_1", "start": 88241, "stop": 88681, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTATTTCATTTTCTTCTTTACTATCAAAATTAGCTTTTAAATTTTCTGCTTTACTTATTTTTGATATTTGGTGGTATTCTTCTGGCGTTGAAAAAATTTTAATAATGTTAAAAAGTGCAGGTGTTGCCATAAATACAGGTAAAGTTATACCAAGTAATGCTTTAGATGAAACTTTTGAAAAAATAATTGAAATAAATGAAATTACAAAAGTTCCAATAATAAGAGCTTCAAAAATAAAAGCTATATGTAAAAGTCAAATATTACCAATAAATTTGGTTCCTTTTAGATCATCGGATAATCCTAAAGCTGCAAAAGATATAATAATTGCTGAGAATAATGCATTAATAGTAGCATTAATTACATTGATTAAAAATGCACTAACAAAAGAAGATCAAAACAGTTCAGTTCTTGATACTGGTTTGGAATATAAAACATAA", "aa": "MFISFSSLLSKLAFKFSALLIFDIWWYSSGVEKILIMLKSAGVAINTGKVIPSNALDETFEKIIEINEITKVPIIRASKIKAICKSQILPINLVPFRSSDNPKAAKDIIIAENNALIVALITLIKNALTKEDQNSSVLDTGLEYKT", "aa_hexdigest": "87d9c83fd9661d4200fecd684c50428f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16112.85119999998, "isoelectric_point": 8.990196800231931 }, "id": "OAIKOHHJFB_200", "locus": "GCA000209735_00168" }, { "type": "cds", "sequence": "contig_1", "start": 88636, "stop": 88926, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAAATTATTAAAGTTAAACTTTAAAACAGCATACAAAGACAAGTCAATATTAATTATTAACTTTTTCCTAGTACCGTTTTTACTTTTAATAGGTACCCTTATTATTCTTATTGTATCTCACACAAATTCAGCTCGAGATATAGTTATTTATTATGGAAGCATGTCACCTATTTTAATTATATTGGCCGCAATTATTACGGGTTCTTATATTTTTAAGATGCCTCAAAATGAAGGCTTAACTTATGTTTTATATTCCAAACCAGTATCAAGAACTGAACTGTTTTGA", "aa": "MGKLLKLNFKTAYKDKSILIINFFLVPFLLLIGTLIILIVSHTNSARDIVIYYGSMSPILIILAAIITGSYIFKMPQNEGLTYVLYSKPVSRTELF", "aa_hexdigest": "14d51bf7c06e3b26995d4a4dfff6635b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10844.998199999989, "isoelectric_point": 9.646807289123537 }, "id": "OAIKOHHJFB_201", "locus": "GCA000209735_00169" }, { "type": "cds", "sequence": "contig_1", "start": 89063, "stop": 89329, "strand": "-", "frame": 1, "gene": "rpsO", "product": "30S ribosomal protein S15", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354857.1", "SO:0001217", "UniParc:UPI0001A8C339", "UniRef:UniRef100_C4XDX6", "UniRef:UniRef50_P0C0K0", "UniRef:UniRef90_C4XDX6" ], "nt": "ATGATTACAAAAGAAGAAAAAGCTAAATTAGTTGCTAAGTATGGTGCTAATAAAAAAGACACAGGAAACACATTTGTTCAAATCGCAATTTTAACAGCAGAAATTGAAGATTTGAAAAAACACTTTGCAGCAAATCCTAAAGACAACCACTCACGTCGTGGTTTCTTAGCAAAAATTTCAAAACGTCGTGTTTTATTACAACACCTTAAGTCAAATGACTTAGAAACATACAACAAAGTTTTAGCTGAATTAAACTTAAGAAAATAG", "aa": "MITKEEKAKLVAKYGANKKDTGNTFVQIAILTAEIEDLKKHFAANPKDNHSRRGFLAKISKRRVLLQHLKSNDLETYNKVLAELNLRK", "aa_hexdigest": "ffd16c098c72fee1d0f9208017cda9d8", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C339", "ncbi_nrp_id": "WP_013354857.1", "uniref100_id": "UniRef100_C4XDX6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C339", "RefSeq:WP_013354857.1", "UniRef:UniRef100_C4XDX6" ] }, "ips": { "uniref100_id": "UniRef100_C4XDX6", "uniref90_id": "UniRef90_C4XDX6" }, "psc": { "uniref90_id": "UniRef90_C4XDX6", "gene": "rpsO", "product": "30S ribosomal protein S15", "uniref50_id": "UniRef50_P0C0K0", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P0C0K0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0C0K0" ], "product": "Small ribosomal subunit protein uS15" }, "genes": [ "rpsO" ], "id": "OAIKOHHJFB_202", "locus": "GCA000209735_00170" }, { "type": "cds", "sequence": "contig_1", "start": 89651, "stop": 90070, "strand": "+", "frame": 2, "gene": "rpsL", "product": "30S ribosomal protein S12", "db_xrefs": [ "GO:0000049", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "KEGG:K02950", "RefSeq:WP_013354858.1", "SO:0001217", "UniParc:UPI0001A8C33A", "UniRef:UniRef100_C4XDX7", "UniRef:UniRef50_P92532", "UniRef:UniRef90_A5IZ35" ], "nt": "ATGCCTACAACAAATCAACTTGTTACAAGTGGTAGAGTTTCTAAAGTTCGTAAGCAAAAAGCACCTGCTTTGAACTTAAGTTTCAATACACTAACAAAAGAAGGTCGTAAGTTGCCAGCTCCATTTAAACGTGGTGTATGTACTCGTGTTGCAACTATGACTCCTAAAAAACCTAACTCAGCTATGCGTAAATATGCTCGTGTTAAGTTATCAAATGGTATGGAAGTTACTGCCTACATTGGTGGTGAAGGACACAACTTACAAGAACACTCAGTTGTTCTTATTCGTGGTGGTAGAGTAAAGGATTTACCTGGTGTTAGATACCACATCGTTCGTGGTACTCAAGATTTAGCTGGTGTTAATAACCGTAAACAAGGACGTAGTCTTTACGGAACTAAAAAAGAAAAGGCTGCTAAATAG", "aa": "MPTTNQLVTSGRVSKVRKQKAPALNLSFNTLTKEGRKLPAPFKRGVCTRVATMTPKKPNSAMRKYARVKLSNGMEVTAYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGTQDLAGVNNRKQGRSLYGTKKEKAAK", "aa_hexdigest": "7688e7a0833844e3d3b9aa9f6605bfa5", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001A8C33A", "ncbi_nrp_id": "WP_013354858.1", "uniref100_id": "UniRef100_C4XDX7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C33A", "RefSeq:WP_013354858.1", "UniRef:UniRef100_C4XDX7" ] }, "ips": { "uniref100_id": "UniRef100_C4XDX7", "uniref90_id": "UniRef90_A5IZ35" }, "psc": { "uniref90_id": "UniRef90_A5IZ35", "gene": "rpsL", "product": "30S ribosomal protein S12", "uniref50_id": "UniRef50_P92532", "kegg_orthology_id": "K02950", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "genes": [ "rpsL" ], "id": "OAIKOHHJFB_203", "locus": "GCA000209735_00171" }, { "type": "cds", "sequence": "contig_1", "start": 90096, "stop": 90404, "strand": "+", "frame": 3, "gene": null, "product": "Ribosomal protein S7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920EYB2" ], "nt": "ATGTCAAGAAAACATTCTGCACCTATAAGAGAAGTTTTAGCTGATCCAGTTTTTAACTCAGTATTAGTTACAAAATTAATTAACACTATTATGCTAGATGGTAAAAAATCTATCGCTCAAGATATCCTTTATTCAGCTTTCAATATCATTAAAGAAAAAACAAACAAAGAACCAATGGAAGTGTTTTTAGCTGCTGTGGAAAATGTTACTCCACAACTTGAAGTTAGAACACGTAGGATTGGTGGAACAAACTATCAAGTTCCAACAGAAGTTTCAGATCGTCGTAAACAAACATTAAGTTTAAGATGA", "aa": "MSRKHSAPIREVLADPVFNSVLVTKLINTIMLDGKKSIAQDILYSAFNIIKEKTNKEPMEVFLAAVENVTPQLEVRTRRIGGTNYQVPTEVSDRRKQTLSLR", "aa_hexdigest": "a0f42759ce6eb781d4da3fa6591175b7", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A2W4JVM3", "query_cov": 0.9901960784313726, "subject_cov": 0.8145161290322581, "identity": 0.505, "score": 111.0, "evalue": 1.39e-29, "valid": false, "gene": "rpsG", "product": "30S ribosomal protein S7", "uniref50_id": "UniRef50_A0A920EYB2", "kegg_orthology_id": "K02992", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_A0A920EYB2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920EYB2" ], "product": "Ribosomal protein S7" }, "genes": [], "id": "OAIKOHHJFB_204", "locus": "GCA000209735_00172" }, { "type": "cds", "sequence": "contig_1", "start": 90408, "stop": 90563, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00177.27" ], "nt": "GTGCAATATGCACGTCTTAGAAATGAAAAAACAATGGATGTTAGATTAGCTAACGAAATCATTGATGCATCAAACAAAACAGGTGGCGCAATTAAGAAACGTGAAGACACACACAAAATGGCTGAAGCTAACCGCGCATTTGCACACTTCAGATGA", "aa": "MQYARLRNEKTMDVRLANEIIDASNKTGGAIKKREDTHKMAEANRAFAHFR", "aa_hexdigest": "338a9f9397997fad27c6d53d83c43be9", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A380FBG9", "query_cov": 0.8431372549019608, "subject_cov": 0.86, "identity": 0.7909999999999999, "score": 73.9, "evalue": 1.41e-16, "valid": false, "product": "30S ribosomalprotein S7p (S5e)", "uniref50_id": "UniRef50_A0A645CV26" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00177.27", "name": "Ribosomal_S7", "length": 44, "aa_cov": 0.8627450980392157, "hmm_cov": 1.0, "evalue": 1.3295245225846493e-18, "score": 63.072261810302734, "start": 2, "stop": 45 } ], "seq_stats": { "molecular_weight": 5905.67, "isoelectric_point": 9.983783531188966 }, "id": "OAIKOHHJFB_205", "locus": "GCA000209735_00173" }, { "type": "cds", "sequence": "contig_1", "start": 90578, "stop": 90733, "strand": "+", "frame": 2, "gene": "lepA", "product": "GTP-binding protein lepA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KNQ4", "UniRef:UniRef90_A0A3P8KNQ4" ], "nt": "ATGGCAAGACAATATAAATTAGAAGATTACCTTAATATCGGTATTATGGCCCACATTGATGCAGGTAAAACAACAACAACAGAAAGAATTTTGCTTCACACAGGTAAAATTCACAAATTAGGTGAAACCCACGACGGTGCTAGCCAAATGGACTGA", "aa": "MARQYKLEDYLNIGIMAHIDAGKTTTTERILLHTGKIHKLGETHDGASQMD", "aa_hexdigest": "f4783571c42bba6996e71a462a482f55", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003516DBAA", "query_cov": 1.0, "subject_cov": 0.9622641509433962, "identity": 0.706, "score": 80.5, "evalue": 3.84e-19, "valid": false, "product": "GTP-binding protein", "uniref50_id": "UniRef50_A0A962BSW7" }, "genes": [ "lepA" ], "truncated": "3-prime", "pseudogene": { "start": 90578, "stop": 90798, "observations": { "deletion": [ 90782 ], "mutation": [ 90731 ], "stop": [ 90731 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8KNQ4", "query_cov": 1.0, "subject_cov": 0.6891891891891891, "identity": 0.98, "score": 105.0, "evalue": 7.9e-29, "gene_start": 1, "gene_end": 51, "reference_start": 1, "reference_end": 51, "reference_sequence": "MARQYKLEDYRNIGIMAHIDAGKTTTTERILLHTGKIHKLGETHDGASQMDWMAQEQERGITITSAATNSILKW" }, "paralog": false, "identity": 0.9726027397260274, "score": 135.0, "evalue": 4.49e-43, "description": "Internal stop codon at 90731. Frameshift due to deletion around 90782. Nonsense mutation around 90731.", "psc": { "uniref90_id": "UniRef90_A0A3P8KNQ4", "product": "GTP-binding protein lepA", "uniref50_id": "UniRef50_A0A3P8KNQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8KNQ4", "UniRef:UniRef50_A0A3P8KNQ4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KNQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KNQ4" ], "product": "GTP-binding protein lepA" } }, "id": "OAIKOHHJFB_206", "locus": "GCA000209735_00174" }, { "type": "cds", "sequence": "contig_1", "start": 90986, "stop": 92101, "strand": "+", "frame": 2, "gene": null, "product": "Elongation factor G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A379SQ57" ], "nt": "ATGGATAAAGCAGGAGCTGACTTCTTTGCAGCTGTTAAATCAGTTAAAGAAAGATTAAACGGTAATGCAGTTGCAATTCAAATTCCTATTGGAGCAGAAGCTCATTTCAAAGGTTTAGTTGACCTTGTTGAAATGAAAGCTTATGAATACGATGGAAAACCTGAAGAAAATGCAAAAGAAATTGTTATTCCTTCAGACTTAGCTGAATTAGCAAAAACAAAAAGACAAGAATTAATTGAAGCTGTTGCTTCATATGATGATGACTTTATGATGCAAGTGCTTGAAGGTGTTGAACCTGATTTAGCAACACTTAAAAAAGTTATTCGTAAAGCAACCCTTACAGCTGAATTCTATCCAGCAGTTTGTGGAACAAGTTTCAAAAACAAAGGTGTTAAGAAAATGATCGACGCAGTTGTTGATTACTTACCATCACCTTTAGATATTCCAGCTACAAAAGCTCACTTAGATGATAAAGAAGTAGCTATTCCAGCTACAGATGATCATGACTTTATTGGTTTAGCATTCAAAGTTATGACTGACCCTTATGTTGGTTCATTAACATTCGTTCGTGGATATGCAGGTATTCTTAATAAGGGATCATATGTATATAACTCAACTAAAGATGTTAAAGAAAGAATTGGACGTTTAATCCAAATGCATGCAAACAGCCGTCAAGAAATTGATGAATGTTATGCAGGTGATATTGTGGCAGCTGTTGGTCTTAAATCAACAACCACAGGTGACACATTAGTTGCTGAAAAATCTCCAAAATACATTTTGGAAAAAATGATTTTCCCTGAACCGGTTATTTCACAAGCTCTTGAACCTGAAAGTAAAGATGCTATGGAAAAACTTGCTTTAGGACTTCAAAAATTAGCTGCTGAAGACCCTACATTTAGAACATACACAGATGAAGAAACAGGTCAAACAATTATTGCTGGTATGGGTGAATTACACCTTGACATTATCGTTGACCGTCTCAAACGTGAACATGGTGTTAAAGCTAAAGTTGGTGCACCTCAAGTTTCATACCGTGAAACAATTACAAAAAGTGCTGAAGTTGAAGGTAAACACATTAAACAATCTGGTGGTAAAGGTCAATATGGTCACGTATGA", "aa": "MDKAGADFFAAVKSVKERLNGNAVAIQIPIGAEAHFKGLVDLVEMKAYEYDGKPEENAKEIVIPSDLAELAKTKRQELIEAVASYDDDFMMQVLEGVEPDLATLKKVIRKATLTAEFYPAVCGTSFKNKGVKKMIDAVVDYLPSPLDIPATKAHLDDKEVAIPATDDHDFIGLAFKVMTDPYVGSLTFVRGYAGILNKGSYVYNSTKDVKERIGRLIQMHANSRQEIDECYAGDIVAAVGLKSTTTGDTLVAEKSPKYILEKMIFPEPVISQALEPESKDAMEKLALGLQKLAAEDPTFRTYTDEETGQTIIAGMGELHLDIIVDRLKREHGVKAKVGAPQVSYRETITKSAEVEGKHIKQSGGKGQYGHV", "aa_hexdigest": "7bf6df4d164d87d183a46180eabbe2f6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00255A9B97", "query_cov": 0.9892183288409704, "subject_cov": 1.0027322404371584, "identity": 0.504, "score": 356.0, "evalue": 1.78e-118, "valid": false, "product": "translation factor GTPase family protein", "uniref50_id": "UniRef50_A0A379SQ57" }, "pscc": { "uniref50_id": "UniRef50_A0A379SQ57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A379SQ57" ], "product": "Elongation factor G" }, "genes": [], "id": "OAIKOHHJFB_207", "locus": "GCA000209735_00175" }, { "type": "cds", "sequence": "contig_1", "start": 92213, "stop": 92668, "strand": "+", "frame": 2, "gene": null, "product": "Elongation factor G domain protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PQ45" ], "nt": "ATGGCTGGTGGTATCTTAGCAGGATATCCTATGATTGATATTAAAGCTACATTATTTGATGGATCATACCATGATGTCGACTCATCAGAATTAGCTTACAAAATTGCTGCATCTAAAGCTTTAACCAAAGCTAAAGACTTATTAGGTACAGTATTACTTGAACCTATTATGGACGTTGCTGTAGTTATTCCTGAAGATTACTTTGGTGATGTTATGGGTGACTTAACACGTCGTCGTGGACAAATTGAAGACAATGAAACAAGAAATGATGGAGCTCACATTATTCGTTCATTAGTTCCTCTTAAAGAAATGTTTGGTTATGCTACTGACTTACGTAGTATGACAGCTGGTCGTGGAACATACCAAATGCAATTCCACCACTATGAAAAATGTCCAAAAAATATTGCAGATGAAATTATCAAACGTCGTAACATTCAAGTTAAAGACGAAGATTAA", "aa": "MAGGILAGYPMIDIKATLFDGSYHDVDSSELAYKIAASKALTKAKDLLGTVLLEPIMDVAVVIPEDYFGDVMGDLTRRRGQIEDNETRNDGAHIIRSLVPLKEMFGYATDLRSMTAGRGTYQMQFHHYEKCPKNIADEIIKRRNIQVKDED", "aa_hexdigest": "7c133eebb2053ee313796d038b4a2d0c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PQ45", "query_cov": 1.0, "subject_cov": 0.8628571428571429, "identity": 0.7809999999999999, "score": 246.0, "evalue": 1.33e-81, "valid": false, "product": "Elongation factor G domain protein", "uniref50_id": "UniRef50_A0A3B0PQ45" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PQ45", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PQ45" ], "product": "Elongation factor G domain protein" }, "genes": [], "id": "OAIKOHHJFB_208", "locus": "GCA000209735_00176" }, { "type": "cds", "sequence": "contig_1", "start": 93176, "stop": 93313, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDY1", "UniRef:UniRef90_C4XDY1" ], "nt": "ATGTATACTCTTTCTTTAAGTAAAATGAATTTAAGTACTAAAGAAGCTTCGCTTTCAAAATTACTAAATGAAAGTAAACTTGAAACTAAAACTTTAAATACTCCTTTTACATATCAAGACATTGCCCAAATTGCTTGA", "aa": "MYTLSLSKMNLSTKEASLSKLLNESKLETKTLNTPFTYQDIAQIA", "aa_hexdigest": "d889d697825ad7dea3f2ec514fd6c993", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 93077, "stop": 93412, "observations": { "mutation": [ 93128, 93311, 93323, 93368 ], "stop": [ 93128, 93311, 93323, 93368 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDY1", "query_cov": 1.0, "subject_cov": 0.40540540540540543, "identity": 1.0, "score": 82.0, "evalue": 3.33e-19, "gene_start": 1, "gene_end": 45, "reference_start": 34, "reference_end": 78, "reference_sequence": "MKLVLEEYILTGSILSNWDKIQHSNVDRIGMLNMYTLSLSKMNLSTKEASLSKLLNESKLETKTLNTPFTYQDIAQIAWKDGWPSLINNNSNALVQIWFDYYINNIMNLDE" }, "paralog": false, "identity": 0.963963963963964, "score": 203.0, "evalue": 1.89e-69, "description": "Internal stop codon at 93128, 93311, 93323, 93368. Nonsense mutation around 93128, 93311, 93323, 93368.", "psc": { "uniref90_id": "UniRef90_C4XDY1", "uniref50_id": "UniRef50_C4XDY1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDY1", "UniRef:UniRef50_C4XDY1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDY1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDY1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5066.796700000002, "isoelectric_point": 8.064301872253417 }, "id": "OAIKOHHJFB_209", "locus": "GCA000209735_00177" }, { "type": "cds", "sequence": "contig_1", "start": 93563, "stop": 93799, "strand": "+", "frame": 2, "gene": null, "product": "DUF4143 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000F03D707", "UniRef:UniRef90_UPI000F03D707" ], "nt": "TTGAATATTGTTTCAAAATTATTTATTATTGAAAAACAAGAATCATATATTCCAAATTATTTGTCAAGTAAAAAAGTTTTAAATACTGCAAAATTAAGATTTATTGATTCTTCTATTCCAATAAACATTTTAGGCTTAACACCCAAATTTCTTGTTGATAATCCAAACATGTTAGGTGTCTTTTTTGAATCATTAGTTATTAGAGACTTGCGCGTTTATGGTCAATTATTTAATTGA", "aa": "MNIVSKLFIIEKQESYIPNYLSSKKVLNTAKLRFIDSSIPINILGLTPKFLVDNPNMLGVFFESLVIRDLRVYGQLFN", "aa_hexdigest": "7361005b9c6b6c11cf413be80a4df78c", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 93536, "stop": 94039, "observations": { "mutation": [ 93797, 94010 ], "stop": [ 93797, 94010 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000F03D707", "query_cov": 1.0, "subject_cov": 0.46706586826347307, "identity": 0.987, "score": 154.0, "evalue": 1.25e-46, "gene_start": 1, "gene_end": 78, "reference_start": 10, "reference_end": 87, "reference_sequence": "MDTRTIDDYLNIVSKLFIIEKQESYIPNYLSSKKVLNTAKLRFIDSSIPINILGLTPKFLVDNPNMLGVFFESLVIRDLRVYGQLFNWNILHYRDKWSNEVDCILQLKNGDFAAIKIKLSNSYEEQTATGLLRFKKETEEEQKLAKINREKMYKKVPKWLIIISGTA" }, "paralog": false, "identity": 0.9760479041916168, "score": 318.0, "evalue": 1.03e-113, "description": "Internal stop codon at 93797, 94010. Nonsense mutation around 93797, 94010.", "psc": { "uniref90_id": "UniRef90_UPI000F03D707", "product": "DUF4143 domain-containing protein", "uniref50_id": "UniRef50_UPI000F03D707", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000F03D707", "UniRef:UniRef50_UPI000F03D707" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000F03D707", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000F03D707" ], "product": "DUF4143 domain-containing protein" } }, "id": "OAIKOHHJFB_210", "locus": "GCA000209735_00178" }, { "type": "cds", "sequence": "contig_1", "start": 93848, "stop": 94012, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAACTTAAAAATGGAGACTTTGCAGCCATTAAAATTAAATTAAGTAATAGTTATGAAGAGCAAACAGCTACAGGCTTACTTAGGTTTAAAAAAGAAATGGAAGAAGAACAAAAATTAGCTAAAATCAATAGGGAAAAAATGTACAAAAAAGTACCTAAATGA", "aa": "MQLKNGDFAAIKIKLSNSYEEQTATGLLRFKKEMEEEQKLAKINREKMYKKVPK", "aa_hexdigest": "8bd64d598b7c0ce0390fdad9750648b8", "start_type": "TTG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6376.428400000001, "isoelectric_point": 9.646807289123537 }, "id": "OAIKOHHJFB_211", "locus": "GCA000209735_00179" }, { "type": "cds", "sequence": "contig_1", "start": 94145, "stop": 94516, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDY3", "UniRef:UniRef90_C4XDY3" ], "nt": "ATGGCTTTTATCTTTTATTTGTGCATCTTTATTATGCCAATTTACAAAGGTTTAGAGTATAAATATGACAATAATATAATGAAGCAAACTAAAGATTTAGCGGCGACTGCAGCAGCATTTTGTATGATAGTTGTAATTGTTTATTTAAGTATTACAGTTGAATCATTTCTAATCTTGAAAAAGTTAATAAAATTCCCTGAAGTAACAGCTGAAATTTATTATATGACTTTTCTTTTTCATAATGATCGTACAATGTTCAAAAATTATTTTGAAAGTGAAAAAATTAATCTAGATCCAAGAATTAAAAAAGAAATTATGGACATAGTTTCTAATGAATCAAAAGCCAAAGGAATAAAGATCATTTTTAGATAA", "aa": "MAFIFYLCIFIMPIYKGLEYKYDNNIMKQTKDLAATAAAFCMIVVIVYLSITVESFLILKKLIKFPEVTAEIYYMTFLFHNDRTMFKNYFESEKINLDPRIKKEIMDIVSNESKAKGIKIIFR", "aa_hexdigest": "7c54bb72b7a9c82fba4767c6508ee47e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 94145, "stop": 94816, "observations": { "mutation": [ 94573, 94648, 94759 ], "stop": [ 94573, 94648, 94759 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDY3", "query_cov": 1.0, "subject_cov": 0.5394736842105263, "identity": 1.0, "score": 237.0, "evalue": 1.11e-77, "gene_start": 1, "gene_end": 123, "reference_start": 106, "reference_end": 228, "reference_sequence": "MLETFNQEAQKYHTEEVKLRRDLSWSSLFAFISLLVAFTITVTFLSLALKNYDYYLSGLNYWYNFFVYIATSSIISLITLILACYFWIKFDRKGVSKYNIIASCLMAFIFYLCIFIMPIYKGLEYKYDNNIMKQTKDLAATAAAFCMIVVIVYLSITVESFLILKKLIKFPEVTAEIYYMTFLFHNDRTMFKNYFESEKINLDPRIKKEIMDIVSNESKAKGIKIIFR" }, "paralog": false, "identity": 0.9865470852017937, "score": 427.0, "evalue": 4.2e-155, "description": "Internal stop codon at 94573, 94648, 94759. Nonsense mutation around 94573, 94648, 94759.", "psc": { "uniref90_id": "UniRef90_C4XDY3", "uniref50_id": "UniRef50_C4XDY3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDY3", "UniRef:UniRef50_C4XDY3" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDY3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDY3" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 14554.295399999986, "isoelectric_point": 9.001543235778808 }, "id": "OAIKOHHJFB_212", "locus": "GCA000209735_00180" }, { "type": "cds", "sequence": "contig_1", "start": 95100, "stop": 96194, "strand": "+", "frame": 3, "gene": "metK", "product": "methionine adenosyltransferase", "db_xrefs": [ "EC:2.5.1.6", "GO:0004478", "GO:0005524", "GO:0006556", "KEGG:K00789", "SO:0001217", "UniRef:UniRef50_P72871", "UniRef:UniRef90_C4XDY4" ], "nt": "ATGAACTTAAATAAACAAATTTTATTCACAAGCGAATCAGTTGGACAAGGGCATCCTGATAAAATTTGTGATCAAATTAGCGATGCAATTTTAGATGAATATTTAAGAAAAGATCCTTATTCTCGTTGTGCTATTGAAACTATGGCTACAGGTAATATGCTAATTATTGCAGGTGAAGCAAGCTCAAAAGCAAATGTTGATCATAAGCAGATTGCGGTTAATGTATTAAAGAAAGCGGGTCAATATTCAGATCATTTAAGATTCCATCTTGATGTTAAAAAACAAAGTACAGATATAAGTCAAGGTGTTATTAGAGCCAATGAAGAAATTGGTGCAGGTGACCAAGGAATTATGTTTGGTTATGCAACAAATGAAACAAGAAACTATATGCCTTTAGCTATTACATTAGCTCATGAAATTGTTAAACGTGCTGATAAATTAAGAGAACGAGGCCTATTTAAATATGCTAAAAGTGATATGAAAAGTCAAGTGACTTTAGACTATACAGATAGTAAAAGAATTAAAGTTGATACTATTTTATTTTCATGTCAACATGAAGAAAACTTTAATGAAAAAGAATTTAAAGAATATATTGTAAAGAATATCTTTGAGCCTGTTTTACAGGACTATTCGTTAAAATTGCCTGTTAAAATTTTAATCAATCCTACAGGGCGTTTTGTTATAGGCGGACCTATTGGAGACACTGGCTTAACAGGTCGTAAAATCATAGTAGATACTTACGGTGGAGCAGCTCGTCATGGTGGCGGAGCTTTTAGTGGTAAGGATGCGACTAAGGTTGATAGATCAGCAGCTTACGCATGTCGTTATGTAACTAAAAATTTAGTTGCTGCAGGTTGTGCAGATAAAATTGAATTACAAGTTTCTTATGCTATTGGTGTTGCTGAACCTGTTTCAATTTTAGTTGAAACTTTTGGTACAGAAAAAATCGCAAAGGATAAAATTGTTTCAATTGTTCATAAACTTTTTGACTTACGTCCAAAACACATTATTGAAAGTTTAAATTTAAGAAGACCTATTTATCAACAAACCTCATACTTTGGACACTTTGGTCGTGAAGATTTAGATCTCCCTTGA", "aa": "MNLNKQILFTSESVGQGHPDKICDQISDAILDEYLRKDPYSRCAIETMATGNMLIIAGEASSKANVDHKQIAVNVLKKAGQYSDHLRFHLDVKKQSTDISQGVIRANEEIGAGDQGIMFGYATNETRNYMPLAITLAHEIVKRADKLRERGLFKYAKSDMKSQVTLDYTDSKRIKVDTILFSCQHEENFNEKEFKEYIVKNIFEPVLQDYSLKLPVKILINPTGRFVIGGPIGDTGLTGRKIIVDTYGGAARHGGGAFSGKDATKVDRSAAYACRYVTKNLVAAGCADKIELQVSYAIGVAEPVSILVETFGTEKIAKDKIVSIVHKLFDLRPKHIIESLNLRRPIYQQTSYFGHFGREDLDLP", "aa_hexdigest": "13f77e9da3324e0f50c0b51c052ed3c0", "start_type": "ATG", "rbs_motif": "GGAGG", "psc": { "uniref90_id": "UniRef90_C4XDY4", "query_cov": 1.0, "subject_cov": 0.9405684754521964, "identity": 1.0, "score": 715.0, "evalue": 5.39e-260, "valid": true, "gene": "metK", "product": "methionine adenosyltransferase", "ec_ids": [ "2.5.1.6" ], "uniref50_id": "UniRef50_P72871", "kegg_orthology_id": "K00789", "go_ids": [ "GO:0004478", "GO:0005524", "GO:0006556" ] }, "pscc": { "uniref50_id": "UniRef50_P72871", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P72871" ], "product": "S-adenosylmethionine synthase" }, "genes": [ "metK" ], "id": "OAIKOHHJFB_213", "locus": "GCA000209735_00181" }, { "type": "cds", "sequence": "contig_1", "start": 96318, "stop": 96434, "strand": "+", "frame": 3, "gene": null, "product": "MarR-family transcription repressor", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C342", "UniRef:UniRef100_C4XDY5", "UniRef:UniRef50_C4XDY5", "UniRef:UniRef90_C4XDY5" ], "nt": "ATGCGTAAAATTGTTAGAGCAAAAAACAAAGAACGTGCAGCTAAAAGACTTCACAAATTACAACTTGAAGAAGCAAGAGATAAAAGAAGAGCAGCAAGAGAAGCTGAAGCAAAATAA", "aa": "MRKIVRAKNKERAAKRLHKLQLEEARDKRRAAREAEAK", "aa_hexdigest": "aa1de1c111f157ffb37add61c45210a8", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001A8C342", "uniref100_id": "UniRef100_C4XDY5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C342", "UniRef:UniRef100_C4XDY5" ] }, "ips": { "uniref100_id": "UniRef100_C4XDY5", "uniref90_id": "UniRef90_C4XDY5" }, "psc": { "uniref90_id": "UniRef90_C4XDY5", "product": "MarR-family transcription repressor", "uniref50_id": "UniRef50_C4XDY5" }, "pscc": { "uniref50_id": "UniRef50_C4XDY5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDY5" ], "product": "MarR-family transcription repressor" }, "genes": [], "id": "OAIKOHHJFB_214", "locus": "GCA000209735_00182" }, { "type": "cds", "sequence": "contig_1", "start": 96744, "stop": 97115, "strand": "-", "frame": 3, "gene": "rplL", "product": "50S ribosomal protein L7/L12", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354867.1", "SO:0001217", "UniParc:UPI0001A8C361", "UniRef:UniRef100_C4XDY6", "UniRef:UniRef50_Q5ZZS0", "UniRef:UniRef90_A0A3P8K8J4" ], "nt": "ATGGCAAAATTAACAAAAGAATCATTCATTTCATCATTAAAAGAAATGTCAATTAAAGAAGTTATGGAATTAGTTGAAGCAATGAAAGAAGAATTTGGAATTGACCCATCAGCTGTTGCTGTTGCTGCTGCTCCTGCTGCAGCTGCAGCTGCTGAAGGTCCTTCAGTAGTTACAGTTTCAATTACTTCAGACAATGGTAAAAAATTACCTATTGTTAAAGCAGTTAAAGAATTATTAAACTTAGCTTTAATGGATGCAAACAAATTAGTTTCAAACCTTCCAGCTGTTGTTAAAGAAAACATCAAACCAGATGAAGCAGAAGCTATCAAAGCTAAACTTGTTGAAGCTGGTGCTACAGTTGAAGTTAAATAA", "aa": "MAKLTKESFISSLKEMSIKEVMELVEAMKEEFGIDPSAVAVAAAPAAAAAAEGPSVVTVSITSDNGKKLPIVKAVKELLNLALMDANKLVSNLPAVVKENIKPDEAEAIKAKLVEAGATVEVK", "aa_hexdigest": "ef67351208754f1f940e309ae7ffc49c", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001A8C361", "ncbi_nrp_id": "WP_013354867.1", "uniref100_id": "UniRef100_C4XDY6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C361", "RefSeq:WP_013354867.1", "UniRef:UniRef100_C4XDY6" ] }, "ips": { "uniref100_id": "UniRef100_C4XDY6", "uniref90_id": "UniRef90_A0A3P8K8J4" }, "psc": { "uniref90_id": "UniRef90_A0A3P8K8J4", "gene": "rplL", "product": "50S ribosomal protein L7/L12", "uniref50_id": "UniRef50_Q5ZZS0", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q5ZZS0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5ZZS0" ], "product": "Large ribosomal subunit protein bL12" }, "genes": [ "rplL" ], "id": "OAIKOHHJFB_215", "locus": "GCA000209735_00183" }, { "type": "cds", "sequence": "contig_1", "start": 97137, "stop": 97646, "strand": "-", "frame": 3, "gene": "rplJ", "product": "50S ribosomal protein L10", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015934", "RefSeq:WP_013527046.1", "SO:0001217", "UniParc:UPI0001F59855", "UniRef:UniRef100_C4XDY7", "UniRef:UniRef50_Q98QS1", "UniRef:UniRef90_A0A3P8MDE2" ], "nt": "ATGCAAAAAAGACATGAATCTGCTTTTAGATTAGTTAAAAAAGATACAGTTAAAGAAATTCAAAATAAACTTGAATCTTCACAAGCTCTTGTTATAGCTGAATATCGTGGATTAAGTGTTGCTGAATTGACTTCATTACGTCAAGAAGCTAAAAAAGAAGGTATTGACATTAAAGTTTACAAAAACCGTCTTTTCAAAATAGCTGCTAAAAATCATGGCATTAATGATTTAGATCAACACTTAGTAGGACCAAACATTTTTGCTTTTGGTACAACCGATGCTCTAATTGCTGCAAAAGTTTTAGTTAAATTCTCTAAAGAAAACAAACTTTTGGTTCCTAAAGCTGGTATTTTTGAAGGCAAAGTTATCGATGCTAAAGGTGTTCAAGAAGTTGCTTCATTACCAAACTATGAAGAAGCTCTTACAATTCTTGCTCGTTCACTTATGTCACCATTACAACAACTCTCTCTTTCTCTAAAAATGTTTAGTGAAAAAGAAGAAAACAAATAA", "aa": "MQKRHESAFRLVKKDTVKEIQNKLESSQALVIAEYRGLSVAELTSLRQEAKKEGIDIKVYKNRLFKIAAKNHGINDLDQHLVGPNIFAFGTTDALIAAKVLVKFSKENKLLVPKAGIFEGKVIDAKGVQEVASLPNYEEALTILARSLMSPLQQLSLSLKMFSEKEENK", "aa_hexdigest": "93bac3e43720b579ced83327b01eaf7c", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI0001F59855", "ncbi_nrp_id": "WP_013527046.1", "uniref100_id": "UniRef100_C4XDY7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59855", "RefSeq:WP_013527046.1", "UniRef:UniRef100_C4XDY7" ] }, "ips": { "uniref100_id": "UniRef100_C4XDY7", "uniref90_id": "UniRef90_A0A3P8MDE2" }, "psc": { "uniref90_id": "UniRef90_A0A3P8MDE2", "gene": "rplJ", "product": "50S ribosomal protein L10", "uniref50_id": "UniRef50_Q98QS1", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QS1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QS1" ], "product": "Large ribosomal subunit protein uL10" }, "genes": [ "rplJ" ], "id": "OAIKOHHJFB_216", "locus": "GCA000209735_00184" }, { "type": "cds", "sequence": "contig_1", "start": 98053, "stop": 98604, "strand": "-", "frame": 2, "gene": null, "product": "Hemolysin C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDY8", "UniRef:UniRef90_C4XDY8" ], "nt": "ATGGCTCAAAATGCTTTAGACTTAGATAGTACCAAAGTTAAAAAACACTACATAAGATTAAAAGATGTATCTATAATTGATGCTAATGCTAATATTCAAGAAGCATTTGAAATTTTTAAAGATACAAACTATAGCCGTATTCCTGTTAAAAAAGATGAAAATTTAATTGGCATTTTACACTTAAAAGATGTCTTCTTTTTACAAAAAGGTAAAGTTATAAACTATTTTAAAACTGTGCCTACTATTAGTGCTAATGCTTCTCTTTCTTCGGCTCTAGAAAAAATGAGGTACCAAAGAGCTCAAATGGCTTTTGTTACACCAAATAACAACTCAAGTGAAATAATTGGAATTATTACAATTGAAGACATTATTGAAGAAATTGTTGGCGAAATTTATGATGAATTTGATGATGAAGAATTAAAAGACATTTTTGAAATTAGTCTTGAACACTTCCATGTCTCAGCTTCTTTAACAATGAAAGAATTAATTAAAAAACTAGAAATAGAATTAGATCTTGAAGAAGAAGAAAATGAATTAACACTTCATAAATGA", "aa": "MAQNALDLDSTKVKKHYIRLKDVSIIDANANIQEAFEIFKDTNYSRIPVKKDENLIGILHLKDVFFLQKGKVINYFKTVPTISANASLSSALEKMRYQRAQMAFVTPNNNSSEIIGIITIEDIIEEIVGEIYDEFDDEELKDIFEISLEHFHVSASLTMKELIKKLEIELDLEEEENELTLHK", "aa_hexdigest": "89d118c33660164ee6c9df534fe2acf8", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 97870, "stop": 98904, "observations": { "mutation": [ 98055, 98730 ], "stop": [ 98055, 98730 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDY8", "query_cov": 1.0, "subject_cov": 0.4255813953488372, "identity": 1.0, "score": 346.0, "evalue": 1.33e-116, "gene_start": 1, "gene_end": 183, "reference_start": 187, "reference_end": 369, "reference_sequence": "MDPKLKLGLSIFGLVILLIASGIFSASETAYTSLNVGKIETMVGNKEFGHKIIKKQHTFFNQTLSTILICNNIVNIAASALTSWLLSTYLGKKFESYNVLISTAVMTPIIVLFGEITPKLIAKSKPEATVKVFCYLLIILYYLFWPITYPISKIGKKIYITNTENDVKNIIDIAQNEGVLEANESIMAQNALDLDSTKVKKHYIRLKDVSIIDANANIQEAFEIFKDTNYSRIPVKKDENLIGILHLKDVFFLQKGKVINYFKTVPTISANASLSSALEKMRYQRAQMAFVTPNNNSSEIIGIITIEDIIEEIVGEIYDEFDDEELKDIFEISLEHFHVSASLTMKELIKKLEIELDLEEEENELTLHKWLEKRLGHKLYKNTKYEYNDVYFKVIDLGTNRFKNVRVDIELGNKADILNTDETLTDNEKN" }, "paralog": false, "identity": 0.9941860465116279, "score": 664.0, "evalue": 2.31e-244, "description": "Internal stop codon at 98055, 98730. Nonsense mutation around 98055, 98730.", "psc": { "uniref90_id": "UniRef90_C4XDY8", "product": "Hemolysin C", "uniref50_id": "UniRef50_C4XDY8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDY8", "UniRef:UniRef50_C4XDY8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDY8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDY8" ], "product": "Hemolysin C" } }, "id": "OAIKOHHJFB_217", "locus": "GCA000209735_00185" }, { "type": "cds", "sequence": "contig_1", "start": 98911, "stop": 99162, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01595.25" ], "nt": "ATGGACCCGAAACTGAAATTAGGATTAAGCATATTCGGACTAGTTATTTTGTTAATAGCTAGCGGAATTTTTAGTGCCAGTGAAACTGCTTATACATCCTTAAATGTTGGCAAAATTGAAACAATGGTTGGCAATAAAGAATTTGGCCACAAAATAATCAAAAAACAACATACATTTTTCAACCAAACTTTAAGTACTATTTTAATATGCAACAATATAGTTAATATTGCTGCTTCGGCCTTAACATCCTGA", "aa": "MDPKLKLGLSIFGLVILLIASGIFSASETAYTSLNVGKIETMVGNKEFGHKIIKKQHTFFNQTLSTILICNNIVNIAASALTS", "aa_hexdigest": "4539d5b404e9c43d6236a0c757912a45", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01595.25", "name": "CNNM", "length": 69, "aa_cov": 0.8313253012048193, "hmm_cov": 1.0, "evalue": 1.6542364074868342e-18, "score": 63.04677200317383, "start": 14, "stop": 82 } ], "seq_stats": { "molecular_weight": 8940.45019999999, "isoelectric_point": 9.302997398376462 }, "id": "OAIKOHHJFB_218", "locus": "GCA000209735_00186" }, { "type": "cds", "sequence": "contig_1", "start": 99249, "stop": 99599, "strand": "-", "frame": 3, "gene": null, "product": "Replicative DNA helicase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W1AXE1" ], "nt": "TTGATTTCATCAGACTCAAATAGAAGAAATGAAAGTCGGCAAAATGAAATAGCTAAAATTAGCCGTTCATTAAAAACATTAGCCTTAGAATTAAATGTTCCAATTATTGCACTAAGCCAACTTTCCCGTGAAGTTGAAAAACGTGAAGACAAAAGACCTATTATGTCTGACTTACGTGAATCAGGTAACATTGAACAAGATGCCGATATTGTTATGTTCTTATATCGTGAAAATTATTATAAAAAACAAGTCTCACAAGAAACCAAAGATCTCTTTGGAGATATTGGAGAAAAAATAGATATCATTATTGCAAAACACAGAAATGGACCAACAGGAATTTTTCCATTATGA", "aa": "MISSDSNRRNESRQNEIAKISRSLKTLALELNVPIIALSQLSREVEKREDKRPIMSDLRESGNIEQDADIVMFLYRENYYKKQVSQETKDLFGDIGEKIDIIIAKHRNGPTGIFPL", "aa_hexdigest": "454095d752080a09bbd74ecb7cd29104", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001AC6C2CE", "query_cov": 0.9913793103448276, "subject_cov": 0.8455882352941176, "identity": 0.565, "score": 111.0, "evalue": 3.35e-29, "valid": false, "product": "DnaB-like helicase C-terminal domain-containing protein", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_A0A7W1AXE1", "kegg_orthology_id": "K02314" }, "pscc": { "uniref50_id": "UniRef50_A0A7W1AXE1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7W1AXE1" ], "product": "Replicative DNA helicase" }, "genes": [], "id": "OAIKOHHJFB_219", "locus": "GCA000209735_00187" }, { "type": "cds", "sequence": "contig_1", "start": 99660, "stop": 100589, "strand": "-", "frame": 3, "gene": "dnaB", "product": "replicative DNA helicase", "db_xrefs": [ "EC:5.6.2.3", "GO:0003677", "GO:0003678", "GO:0005524", "GO:0006260", "SO:0001217", "UniRef:UniRef50_C4XDY9", "UniRef:UniRef90_C4XDY9" ], "nt": "ATGCCAAATAATAATCCAAGTAATGCACCAATAAATACATCTCGTCATATTAACTCTAGATATGAGGAGTCTCTTTTAGGCTTAATCTTAACAGACAACAAAAACGCACCTAAAATTGTGCCTTACTTACTTGAAGAAGACTTTGCAATTTTAGAAAATCAAAGACTTTTCTCTGTTTTTAAAGAACTATTTGAAAACAACATTTCGATTAATGAAGAAAACATTTTTACTGTTGCTAAAAAAAGAAACTTTGTTCAAATAACACCTTTATATTTATCTAAACTTTATCATACTGCTGGTTTCTCATCTAATATTCAAACTTACTTAGAAGAATTAGTTAAGTTAACAAAATTACGTTTAATTGAATCACGTTTAGCTTTAGTTCAAAGAAGACTTGATGTTGAAAAAGCAATCAATGAAAAAGATGTTATTTCAGATTTACAAAACCTATTACTTGACATTGATCGTAGTCAAGTTAGTGCAGACTTTTTAACAGCCAAAGAAGTTAGCGATGAATACTTTAAAGATTTAGAAAATCGCCACTCAAGAGATCTAAATGAATTAAATGGACTAGCAACAGGTTACGACAATTTAGACCAAATTACTCAAGGTCTACATGGTGGTGAATTAATTATTATTGCTGCTCGTCCAGCAATGGGTAAAACTGCTTTTGCTCTTAATATTGCTTCAAATGTTGCTAAAAGAAAAAACAGAAGAGCTGTCTTCTTTACGCTTGAAATGTCATCAAACCAACTTATGGGTAGAATTTATAGCTTGAACACAAATGTGCCACTTCAAAAACTAAAACAACCTCAAACAATTACAGATAATGAATTTCTTTTAATCAACAGTTTAAAACAAGATGTAATTGACAAAATGAACTTATTTATAGATGAATCAGTAAATAATGAATTAAATACACTGCTTTGA", "aa": "MPNNNPSNAPINTSRHINSRYEESLLGLILTDNKNAPKIVPYLLEEDFAILENQRLFSVFKELFENNISINEENIFTVAKKRNFVQITPLYLSKLYHTAGFSSNIQTYLEELVKLTKLRLIESRLALVQRRLDVEKAINEKDVISDLQNLLLDIDRSQVSADFLTAKEVSDEYFKDLENRHSRDLNELNGLATGYDNLDQITQGLHGGELIIIAARPAMGKTAFALNIASNVAKRKNRRAVFFTLEMSSNQLMGRIYSLNTNVPLQKLKQPQTITDNEFLLINSLKQDVIDKMNLFIDESVNNELNTLL", "aa_hexdigest": "b6a30272d6c6d868258c3cee7841b824", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [ "dnaB" ], "truncated": "5-prime", "pseudogene": { "start": 99357, "stop": 100589, "observations": { "mutation": [ 99662 ], "stop": [ 99662 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDY9", "query_cov": 1.0, "subject_cov": 0.6518987341772152, "identity": 1.0, "score": 586.0, "evalue": 2.12e-208, "gene_start": 1, "gene_end": 309, "reference_start": 1, "reference_end": 309, "reference_sequence": "MPNNNPSNAPINTSRHINSRYEESLLGLILTDNKNAPKIVPYLLEEDFAILENQRLFSVFKELFENNISINEENIFTVAKKRNFVQITPLYLSKLYHTAGFSSNIQTYLEELVKLTKLRLIESRLALVQRRLDVEKAINEKDVISDLQNLLLDIDRSQVSADFLTAKEVSDEYFKDLENRHSRDLNELNGLATGYDNLDQITQGLHGGELIIIAARPAMGKTAFALNIASNVAKRKNRRAVFFTLEMSSNQLMGRIYSLNTNVPLQKLKQPQTITDNEFLLINSLKQDVIDKMNLFIDESVNNELNTLLWKCRRLHKVSPIDLIVIDYLQLISSDSNRRNESRQNEIAKISRSLKTLALELNVPIIALSQLSREVEKREDKRPIMSDLRESGNIEQDADIVMFLYRENYYKKQVSQETKDLFGDIGEKIDIIIAKHRNGPTGIFPLWFKMSCGRFINFENEQIAVSSDDDEEEE" }, "paralog": false, "identity": 0.9975609756097561, "score": 786.0, "evalue": 9.24e-290, "description": "Internal stop codon at 99662. Nonsense mutation around 99662.", "psc": { "uniref90_id": "UniRef90_C4XDY9", "gene": "dnaB", "product": "replicative DNA helicase", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_C4XDY9", "go_ids": [ "GO:0003677", "GO:0003678", "GO:0005524", "GO:0006260" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDY9", "EC:5.6.2.3", "UniRef:UniRef50_C4XDY9", "GO:0003677", "GO:0003678", "GO:0005524", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDY9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDY9" ], "product": "Replicative DNA helicase" } }, "id": "OAIKOHHJFB_220", "locus": "GCA000209735_00188" }, { "type": "cds", "sequence": "contig_1", "start": 100579, "stop": 101034, "strand": "-", "frame": 2, "gene": "rplI", "product": "50S ribosomal protein L9", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02939", "RefSeq:WP_013527049.1", "SO:0001217", "UniParc:UPI0001A8C365", "UniRef:UniRef100_C4XDZ0", "UniRef:UniRef50_Q4A5Y3", "UniRef:UniRef90_C4XDZ0" ], "nt": "ATGAAAGTAATATTGATTAAAGATTGTGATAAAGGTAAAGCCAATACAATTATTGAAGTTTCAGCTGGTTATGGTAATAACTTCTTAATTGCAAAAGGTTTTGCAGTTGTCTATAATGACAAGACTAAAAAAGAGCTTGAACAAAGACTTAGTGAGCTTACTAAAAATGAAATGGAAAAACGTTCAGAAGCTCTTGAACTTAAAGATCAAATTGAAAAATTAACTTTAAAATATACTTTAGATGCGCATATTGACAATAATGGAAATTTAATTGCTCATGGTGCTGTATCAACCAAAGATATTGTTAAAGATTTAGTCCAAAATGGCTATAAATTAGATAAATACGCAGTTCAAAAAGTTCATTTAGTATCTAATGGATTGCATGATATAGATGTTATTTTATATAAAGATATCATTGCAAAATTGAAAGTAGAAGTGATAATTAATGCCAAATAA", "aa": "MKVILIKDCDKGKANTIIEVSAGYGNNFLIAKGFAVVYNDKTKKELEQRLSELTKNEMEKRSEALELKDQIEKLTLKYTLDAHIDNNGNLIAHGAVSTKDIVKDLVQNGYKLDKYAVQKVHLVSNGLHDIDVILYKDIIAKLKVEVIINAK", "aa_hexdigest": "93fd4b1ac987040e6f87959cbecf40f6", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001A8C365", "ncbi_nrp_id": "WP_013527049.1", "uniref100_id": "UniRef100_C4XDZ0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C365", "RefSeq:WP_013527049.1", "UniRef:UniRef100_C4XDZ0" ] }, "ips": { "uniref100_id": "UniRef100_C4XDZ0", "uniref90_id": "UniRef90_C4XDZ0" }, "psc": { "uniref90_id": "UniRef90_C4XDZ0", "gene": "rplI", "product": "50S ribosomal protein L9", "uniref50_id": "UniRef50_Q4A5Y3", "kegg_orthology_id": "K02939", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5Y3" ], "product": "Large ribosomal subunit protein bL9" }, "genes": [ "rplI" ], "id": "OAIKOHHJFB_221", "locus": "GCA000209735_00189" }, { "type": "cds", "sequence": "contig_1", "start": 101429, "stop": 101929, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01368.27" ], "nt": "TTGGATGCAATCGGTTCAGCTTTAGGAGTTTATGCTATAGCTAAAACATTTGATAAAGAAGCTTACATTTGCACAATGACTCAAGATGTTACAACTAAAAAAGCAATTGCTAAATACTTTGGTGAAGATAATCCAATTTTCATTAAAGCTCATCAAGCTAACACATTGACTGATGAAAATACACTTGTATTTTTAGTTGATAACTCAGCTCCAAATAGGACTGATAATCCAAAATGTGTTGAAAAAGCTAAAAACACAAATATCTTTATTTTAGACCACCATAGATTAAATTCTTCAGTTGACTTTTGTTCAAAAATTAATCGCCACATTGAGCCTTCAGCTTCAAGTGCTAGTGAAATAGTAACTGAATTAATGATGTTTTTAGAAAAGCAAGTTAAAGTTAAAAAGAACATTGCTCAAATGTTATTAAATGGTATTTATCTTGACACCTTACAATTTCAAAAACATGTTACTTCAAGAACATTTGAAGCAGCAAGTTGA", "aa": "MDAIGSALGVYAIAKTFDKEAYICTMTQDVTTKKAIAKYFGEDNPIFIKAHQANTLTDENTLVFLVDNSAPNRTDNPKCVEKAKNTNIFILDHHRLNSSVDFCSKINRHIEPSASSASEIVTELMMFLEKQVKVKKNIAQMLLNGIYLDTLQFQKHVTSRTFEAAS", "aa_hexdigest": "9b3686e0e379f9225b4e17774aef36eb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01368.27", "name": "DHH", "length": 151, "aa_cov": 0.8795180722891566, "hmm_cov": 0.7549668874172185, "evalue": 2.0342273222342313e-19, "score": 66.18602752685547, "start": 1, "stop": 146 } ], "seq_stats": { "molecular_weight": 18592.078499999996, "isoelectric_point": 6.955978584289551 }, "id": "OAIKOHHJFB_222", "locus": "GCA000209735_00190" }, { "type": "cds", "sequence": "contig_1", "start": 101996, "stop": 102151, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF24898.1" ], "nt": "ATGGAACAAGCTAAAAATGCTTTATTACAAGCTCAAAGTCGTGGTGGTGACCAAGTAGCTATTTTCTCAAGAAATAATTTACCTCGTTACTTTGGTTCAACAAGTGAAATCTTGCCAAATATCAACCGTGCAAGAATCAAAGCACTTGCTAATTGA", "aa": "MEQAKNALLQAQSRGGDQVAIFSRNNLPRYFGSTSEILPNINRARIKALAN", "aa_hexdigest": "e3af2c8c559a26432e1e324b3da8177b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF24898.1", "name": "GGDEF_GdpP", "length": 26, "aa_cov": 0.5098039215686274, "hmm_cov": 1.0, "evalue": 1.5291410532605513e-09, "score": 34.28099060058594, "start": 2, "stop": 27 } ], "seq_stats": { "molecular_weight": 5644.3451, "isoelectric_point": 10.877960014343262 }, "id": "OAIKOHHJFB_223", "locus": "GCA000209735_00191" }, { "type": "cds", "sequence": "contig_1", "start": 102158, "stop": 102352, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF24898.1" ], "nt": "GTGTGTGATTACAACTTAGTTTATCGTCAATATAATACAAACGGTAAATTTCTTATTGTAACTAACAAGCAAGCTTTAGATAAAATGAGTCAAGTAAACTTTAAGTTTTTTGATGATTTACATAAAACACTAAAAAGTCAAAACATTATTATTTCAGTTTCTGCAGGTTTTGCATATGGAACAAAAAACTTATGA", "aa": "MCDYNLVYRQYNTNGKFLIVTNKQALDKMSQVNFKFFDDLHKTLKSQNIIISVSAGFAYGTKNL", "aa_hexdigest": "68e3dcb4fbc18fedaea0f8016eeccf5f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF24898.1", "name": "GGDEF_GdpP", "length": 62, "aa_cov": 0.96875, "hmm_cov": 0.967741935483871, "evalue": 2.9990092559909426e-18, "score": 62.42011642456055, "start": 3, "stop": 64 } ], "seq_stats": { "molecular_weight": 7399.440099999999, "isoelectric_point": 9.41704196929932 }, "id": "OAIKOHHJFB_224", "locus": "GCA000209735_00192" }, { "type": "cds", "sequence": "contig_1", "start": 102689, "stop": 102985, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTAAAACAAAAAATAATTTTATATTCTTTAATAGCAGTTGCTGGCGTTTTCCTAGCTGTAGGGTCAGTAATTTTGTATTTTACTCGTTCAGATATTTATAGCAGCATTTTTGGAATTTTTGGCATATGTCTTTCATTAATAATCGTTGGATTATTTGGAATTTTATCTGTTAAAAGTTTTTTATCAACTCAAGCTATAGTAAAAAAATCTTTTAACAGTTTCATTGATGAAATCATTAGTCATAATGCAATTGGAGTGCTTATTTTTGACAGTGAAGGCCAAATTCTTTGA", "aa": "MNLKQKIILYSLIAVAGVFLAVGSVILYFTRSDIYSSIFGIFGICLSLIIVGLFGILSVKSFLSTQAIVKKSFNSFIDEIISHNAIGVLIFDSEGQIL", "aa_hexdigest": "7b6b56416ddb61cfdc0263c92ea312e3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10664.598699999986, "isoelectric_point": 7.878761863708497 }, "id": "OAIKOHHJFB_225", "locus": "GCA000209735_00193" }, { "type": "cds", "sequence": "contig_1", "start": 103238, "stop": 103420, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTAATATTGACCACAATAATAATGATTCTAAAAAACTTATAAATTCCTTTAATGAAATGCTTGATTTAAATATTGAAAACAAACTCCAATTTTTTATAGATGAATTTAGAAAAACAACAGATGATAAAATACTAAAATTTGTAGTTATCAAAAAAATGAGTAAGAAAAAAAATTCTTAA", "aa": "MANIDHNNNDSKKLINSFNEMLDLNIENKLQFFIDEFRKTTDDKILKFVVIKKMSKKKNS", "aa_hexdigest": "e48f5d5431332c899e8c89359da58c78", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7148.2021, "isoelectric_point": 9.34754505157471 }, "id": "OAIKOHHJFB_226", "locus": "GCA000209735_00194" }, { "type": "cds", "sequence": "contig_1", "start": 103727, "stop": 103888, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAACATAATGCTTTATCTAGTTAATAATAAATTAACCACTTACAATTCACAGTATATTTTTAGAGAAAAAGACAAACGCTATATCTTTGAAAAAAGCTCCCTAAATGAAATATTAAATAAAAATTTAAATGCAAACACAGGTCAAATTATAACCTGA", "aa": "MKNIMLYLVNNKLTTYNSQYIFREKDKRYIFEKSSLNEILNKNLNANTGQIIT", "aa_hexdigest": "bfacfb99c1df866093beadbb76364c8f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6345.262999999998, "isoelectric_point": 9.630754661560058 }, "id": "OAIKOHHJFB_227", "locus": "GCA000209735_00195" }, { "type": "cds", "sequence": "contig_1", "start": 103925, "stop": 104137, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAAAATTTGAAGCAAATCTAGAATTTTATAACAATTTTTCAGATTGTTTAAACTTTATAGATAGCTTAACAAATGTTTTTCCAAAAGATAACACTTTTTACAAAAAAACCTATTTTACAGTTAATAACGACAATTTGTTCTATATGCAAAACTCAAATCTTTATAAAATAATTTTAAATAAAAGACATCTTGAAAATATGAATACTTGA", "aa": "MEKFEANLEFYNNFSDCLNFIDSLTNVFPKDNTFYKKTYFTVNNDNLFYMQNSNLYKIILNKRHLENMNT", "aa_hexdigest": "7cc9564dcdc57bd2c423cbda59968218", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8537.515099999997, "isoelectric_point": 5.635949897766112 }, "id": "OAIKOHHJFB_228", "locus": "GCA000209735_00196" }, { "type": "cds", "sequence": "contig_1", "start": 105089, "stop": 105223, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5985A", "UniRef:UniRef100_A0AB32XD23", "UniRef:UniRef50_A0AB32XD23", "UniRef:UniRef90_A0AB32XD23" ], "nt": "ATGGAAGATAAAGATTTATTAATTAAACAATTAAAGGAAGAAAATAAAATACTTAAATTGGAAAACGCGATAGCAAAAAAAGTGAGCGTTTTGGTTCAATTGAAAGACTCACTAACAAAGAAAAATTCCAAATAA", "aa": "MEDKDLLIKQLKEENKILKLENAIAKKVSVLVQLKDSLTKKNSK", "aa_hexdigest": "61d7f2fbdd701153f3a3f94a31e5d7c2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F5985A", "uniref100_id": "UniRef100_A0AB32XD23", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5985A", "UniRef:UniRef100_A0AB32XD23" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XD23", "uniref90_id": "UniRef90_A0AB32XD23" }, "psc": { "uniref90_id": "UniRef90_A0AB32XD23", "uniref50_id": "UniRef50_A0AB32XD23" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XD23", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XD23" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5066.009200000001, "isoelectric_point": 9.574538230895993 }, "id": "OAIKOHHJFB_229", "locus": "GCA000209735_00197" }, { "type": "cds", "sequence": "contig_1", "start": 105496, "stop": 105630, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDZ4", "UniRef:UniRef90_C4XDZ4" ], "nt": "ATGATTCAAAAATTTAGACCTGAATTAGTTGGACGTTTTAAAGATATCTCATTTTTGGATAAATTTAATGCTAAACAAGTTAAGGCTTTAATTAACAAAAAAAATCAAAGAAGACGAAATCAATATTTATTGTGA", "aa": "MIQKFRPELVGRFKDISFLDKFNAKQVKALINKKNQRRRNQYLL", "aa_hexdigest": "70c4483868b5529ad3f62102c7b32ddb", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 105358, "stop": 105663, "observations": { "mutation": [ 105628 ], "stop": [ 105628 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDZ4", "query_cov": 1.0, "subject_cov": 0.43564356435643564, "identity": 1.0, "score": 86.7, "evalue": 3.64e-21, "gene_start": 1, "gene_end": 44, "reference_start": 47, "reference_end": 90, "reference_sequence": "MDECKFINYINVGYDCSKLILIFQSNVSQDNYSNNKDKNSQKEQEKMIQKFRPELVGRFKDISFLDKFNAKQVKALINKKNQRRRNQYLLWKSYFKFIYRY" }, "paralog": false, "identity": 0.9801980198019802, "score": 203.0, "evalue": 1.33e-69, "description": "Internal stop codon at 105628. Nonsense mutation around 105628.", "psc": { "uniref90_id": "UniRef90_C4XDZ4", "uniref50_id": "UniRef50_C4XDZ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDZ4", "UniRef:UniRef50_C4XDZ4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDZ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDZ4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5394.352700000001, "isoelectric_point": 11.112367057800292 }, "id": "OAIKOHHJFB_230", "locus": "GCA000209735_00198" }, { "type": "cds", "sequence": "contig_1", "start": 105833, "stop": 106102, "strand": "+", "frame": 2, "gene": null, "product": "HAD family hydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0022A714CF" ], "nt": "TTGTTGTATAATACAAAAACTATGGATAAAAACCAAAAAACATATTTTATAGACTTAGACGGGACTTTATTCGACCAAAGGAAAAAAAGTAGAATCAGTGCTAAAAATATTAGTGCAATTTTATTACTAAAAAATTTTGCTAATGTGGTACTTTCAACAGGGAGAAGTTTTAGTGATCAAAGAGTCCAACAAGCAATTTATCAATTAGGAATCAAGGATGTTATTACTTCTTCTGGAGCTGAAATTTATATTGATAATAATTTAGTTTGA", "aa": "MLYNTKTMDKNQKTYFIDLDGTLFDQRKKSRISAKNISAILLLKNFANVVLSTGRSFSDQRVQQAIYQLGIKDVITSSGAEIYIDNNLV", "aa_hexdigest": "46192d372421775d71caf6ae801fc9ea", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0022A714CF", "query_cov": 0.8539325842696629, "subject_cov": 0.8735632183908046, "identity": 0.618, "score": 102.0, "evalue": 9.09e-27, "valid": false, "product": "HAD family hydrolase", "uniref50_id": "UniRef50_UPI0022A714CF" }, "pscc": { "uniref50_id": "UniRef50_UPI0022A714CF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0022A714CF" ], "product": "HAD family hydrolase" }, "genes": [], "id": "OAIKOHHJFB_231", "locus": "GCA000209735_00199" }, { "type": "cds", "sequence": "contig_1", "start": 106256, "stop": 106693, "strand": "+", "frame": 2, "gene": null, "product": "COF family HAD hydrolase protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDZ5", "UniRef:UniRef90_C4XDZ5" ], "nt": "ATGCATGGTATTAAAAGAAGTAAAAACTTCAATTTTAACAATCACAAGAACATTACAAAAATTGCTTTAATTTTAAAAAATATAGCAGGCGCCAAAAACATATTAAAAACATTCCAAGTTAATTTTGATGATATTTGCAATTCATATTTAGCTAGTGCTAATTATGTTGTTGAAATCACAACTATTGATGCTAATAAAGGATTATCTGAATCTGCTTATTGTGCATTAAAAGGTATTAATCAAAATAATACTATTCATATTGGTGACTCAATGGCTGATGCTTCAGTTAAAGGTTATGTTGGAAAATTAGTGGCAATGGGTAATGCCACTAGTGATCTTAAAGTTGTTGCAGATGAAATAGCTCCTAACTACAAAAGTGGTGGTTTGTATCGTTATTTTGTTTCAAGTAAGGGCAAGAAAGGAAGCAATAATGATTAA", "aa": "MHGIKRSKNFNFNNHKNITKIALILKNIAGAKNILKTFQVNFDDICNSYLASANYVVEITTIDANKGLSESAYCALKGINQNNTIHIGDSMADASVKGYVGKLVAMGNATSDLKVVADEIAPNYKSGGLYRYFVSSKGKKGSNND", "aa_hexdigest": "ceccd4252a15f9e9331a4fd27c9d8dcc", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 105956, "stop": 106693, "observations": { "mutation": [ 106100, 106226 ], "stop": [ 106100, 106226 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDZ5", "query_cov": 1.0, "subject_cov": 0.48494983277591974, "identity": 1.0, "score": 285.0, "evalue": 3.93e-95, "gene_start": 1, "gene_end": 145, "reference_start": 155, "reference_end": 299, "reference_sequence": "MGSFLFANNIHYFLLYNTKTMDKNQKTYFIDLDGTLFDQRKKSRISAKNISAILLLKNFANVVLSTGRSFSDQRVQQAIYQLGIKDVITSSGAEIYIDNNLVWQRTFKKDTANKIQKFAEENKILYVIFDEEGETIYVHNAFDYWINKLFLSKKMHGIKRSKNFNFNNHKNITKIALILKNIAGAKNILKTFQVNFDDICNSYLASANYVVEITTIDANKGLSESAYCALKGINQNNTIHIGDSMADASVKGYVGKLVAMGNATSDLKVVADEIAPNYKSGGLYRYFVSSKGKKGSNND" }, "paralog": false, "identity": 0.9918367346938776, "score": 477.0, "evalue": 4.19e-173, "description": "Internal stop codon at 106100, 106226. Nonsense mutation around 106100, 106226.", "psc": { "uniref90_id": "UniRef90_C4XDZ5", "product": "COF family HAD hydrolase protein", "uniref50_id": "UniRef50_C4XDZ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDZ5", "UniRef:UniRef50_C4XDZ5" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDZ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDZ5" ], "product": "COF family HAD hydrolase protein" } }, "id": "OAIKOHHJFB_232", "locus": "GCA000209735_00200" }, { "type": "cds", "sequence": "contig_1", "start": 106686, "stop": 107234, "strand": "+", "frame": 3, "gene": null, "product": "deoxyribonuclease IV", "db_xrefs": [ "EC:3.1.21.2", "GO:0003677", "GO:0006281", "GO:0008270", "SO:0001217", "UniRef:UniRef50_Q4A6S5", "UniRef:UniRef90_C4XDZ6" ], "nt": "ATGATTAAATTAGGTAGTCACATTTCTTTTAAAAGTCCAGATTATTTAGTTGGCTCAATTAAAGAATCACTTAAAAATAAAGCTAACTGTACCATGATTTATTTGGGCGCTCCTCAGAATGCTAAAAGAGTTGATACAAAAGTTTATAAATATGAAGAATATAAAGCTAATTATTCTAATTTAATTAAACCAGAAGACATTGTAGTTCATGCTCCTTATATTATTAATCCCTCAAGTAAAGATAAGGTTAAAAATGCTTTTGCAGTTGATTTTTTAATTAAAGAAATTCAAAGAATGAATTATATTGGAGTTAAATATTTAGTACTTCATCCAGGCGCTTATACAACTTATGAATTACAAGAATCACTCGCACAATTAGTTAAGTCTTTAAAAGAAGTTTTAGCTAAAACTAAAGATGTTGTTATATGTATTGAAACTATGTCTGGAAAGGGTACTGAAGTTGGTACTAATTTTGCCCAATTACGCTATTTAATAGATGAATTAGCAAATGACAGATTACAAATTTGCTTGGATACATGTCATGTTTGA", "aa": "MIKLGSHISFKSPDYLVGSIKESLKNKANCTMIYLGAPQNAKRVDTKVYKYEEYKANYSNLIKPEDIVVHAPYIINPSSKDKVKNAFAVDFLIKEIQRMNYIGVKYLVLHPGAYTTYELQESLAQLVKSLKEVLAKTKDVVICIETMSGKGTEVGTNFAQLRYLIDELANDRLQICLDTCHV", "aa_hexdigest": "16a1bf1126283fd3cab12f0ebe39ca55", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 106632, "stop": 107525, "observations": { "mutation": [ 107232 ], "stop": [ 107232 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDZ6", "query_cov": 1.0, "subject_cov": 0.6127946127946128, "identity": 1.0, "score": 363.0, "evalue": 3.14e-125, "gene_start": 1, "gene_end": 182, "reference_start": 19, "reference_end": 200, "reference_sequence": "MVVCIVILFQVRARKEAIMIKLGSHISFKSPDYLVGSIKESLKNKANCTMIYLGAPQNAKRVDTKVYKYEEYKANYSNLIKPEDIVVHAPYIINPSSKDKVKNAFAVDFLIKEIQRMNYIGVKYLVLHPGAYTTYELQESLAQLVKSLKEVLAKTKDVVICIETMSGKGTEVGTNFAQLRYLIDELANDRLQICLDTCHVWDAGYNIKNYQEFKSEINKYNLLKHIKVIHLNDSKNDLNSHKDRHENIDKGFIGIDTLAKFVHDKDFDNVPIILETPIPENGPIYDQEIAALLAYKK" }, "paralog": false, "identity": 0.9932659932659933, "score": 590.0, "evalue": 2.81e-217, "description": "Internal stop codon at 107232. Nonsense mutation around 107232.", "psc": { "uniref90_id": "UniRef90_C4XDZ6", "product": "deoxyribonuclease IV", "ec_ids": [ "3.1.21.2" ], "uniref50_id": "UniRef50_Q4A6S5", "go_ids": [ "GO:0003677", "GO:0006281", "GO:0008270" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDZ6", "EC:3.1.21.2", "UniRef:UniRef50_Q4A6S5", "GO:0003677", "GO:0006281", "GO:0008270" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A6S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6S5" ], "product": "Probable endonuclease 4" } }, "id": "OAIKOHHJFB_233", "locus": "GCA000209735_00201" }, { "type": "cds", "sequence": "contig_1", "start": 108073, "stop": 108393, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATATAATTAATGTTATGAAAATTGAAGTTAATAGTGTTAGGAAAAGATTAAAAATTTTCGCTCGTGAAAAAAAGAATGAAAATGACGTAGTTGGCGATGTTAGTTATTCAATTCCAGGAGAAATTTTAAAATTTCTCATTTTGCTTTTTGTTGCCTTAGCTTTTATTATGTTTCAATATCAAGATCTCTTTTTTCACAATGATAATAAAGTAATAAATATTATTCGCAAAATTTATTTATATAGTTTCGGCTTAGCTTTTGGTGACATAATAACACTTGTAATAATAGGATCATTTTTAACACTTATGGTGCGTTGA", "aa": "MYIINVMKIEVNSVRKRLKIFAREKKNENDVVGDVSYSIPGEILKFLILLFVALAFIMFQYQDLFFHNDNKVINIIRKIYLYSFGLAFGDIITLVIIGSFLTLMVR", "aa_hexdigest": "3cf7fa56839314ecec6b0798db4808aa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12384.719499999985, "isoelectric_point": 9.474999046325685 }, "id": "OAIKOHHJFB_234", "locus": "GCA000209735_00202" }, { "type": "cds", "sequence": "contig_1", "start": 108850, "stop": 110130, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDZ7", "UniRef:UniRef90_C4XDZ7" ], "nt": "ATGGTTGAACAATACTTGCAAAAAATAAATAGTTTATTCTACTACACACCTCAAGTTAGAGAACTATTCAGATTCTACAAAGATGCAGCTCAAGTCTTAAAAGTAGATTACTTTAAATTTAATTTTAGATACTTACAAAAATTAGTTAAAAAGAATTTGACTCTTTTAGCTCAACATACCCTCACAGCTAAATACTTTGAAAATGTTAAAGCTAAAAAGACAGCTAGTCAACTTGAAGCAGAAGAATTTCTTAAAATAGTTGAAACTACTCAAACTAATACAACTTCTGCAACAATAACTTTAAATGCAGCAGCTGATATTAAAATGCCAGCAATCAATGCTCAAAATCTTTTAGTTAATAAACATAATAAAAATAATACAAATACATACATTAGCAATGAATCAACAAGTGAATACTCAAAAGATTTTGATTTAACAGAAGAAAGATATATGTCTAATTATGTTGAAACTATTGAGGTCGAAGCTCAAAGTCAAGAATCAACAAAATTATTAATTGATGATTTAGAAAACAAAAAATCAGATACAGAAGAAATCAAAGCTAAAACTAAAGAAATGACTGCTAAATTTGAAGCAGTACAACCTACTGAAGAAAAAACAAAAGAAATCACAAATATTTTATTAGCAGATCAAGTAATTAGTGAAGTAACTAAGGAAGAAAAACTTGCTCCAACTATAGAACAAGTTAGTCAACAATTCTTAGTAGACACCAACGAATTAACAGATACAAATGATGTTGAAAAAGACCAAACTCAAAGCAATATAATTGACCAAAACAATAGTAAAACAGTAAAATTAGTAAAAGAAACCATCGAAAATGAAGAAAGTAAAACCTTAGAACAAGAAATAGCATTCTTAATTGATTCAAGTGAAAATACATTTGTAATGAGTGCTGAAAAGAATACTCAAAATATAACTCAAACAATGAGTCAACCTGTAGAAGTTAAAGCTCAAACTATTGAACAAAAAATAGAAGAAAATGTTGAAAACAAAAAGCAAACTATAGGTTTCATTATTCCTGAAGATTCAAATGAAACAATAATTGAAAAAGATGACGATCTAAAAACTAAAGAAGAATTTATTGCTCAACTAGACAGCATTGAAGCAGATAATTTGGCTTCAAAAGAAATTAAACTTGAACAACCAGAAAGTGAAAAGAGACAAAATTTCCAATTCACTTTTGATACTAATACAATTGTAATCAACAATGATTCAAATAAATCAAAAGATAGAGTAGATCACTTCGAAGATGATAATGATTGA", "aa": "MVEQYLQKINSLFYYTPQVRELFRFYKDAAQVLKVDYFKFNFRYLQKLVKKNLTLLAQHTLTAKYFENVKAKKTASQLEAEEFLKIVETTQTNTTSATITLNAAADIKMPAINAQNLLVNKHNKNNTNTYISNESTSEYSKDFDLTEERYMSNYVETIEVEAQSQESTKLLIDDLENKKSDTEEIKAKTKEMTAKFEAVQPTEEKTKEITNILLADQVISEVTKEEKLAPTIEQVSQQFLVDTNELTDTNDVEKDQTQSNIIDQNNSKTVKLVKETIENEESKTLEQEIAFLIDSSENTFVMSAEKNTQNITQTMSQPVEVKAQTIEQKIEENVENKKQTIGFIIPEDSNETIIEKDDDLKTKEEFIAQLDSIEADNLASKEIKLEQPESEKRQNFQFTFDTNTIVINNDSNKSKDRVDHFEDDND", "aa_hexdigest": "b08ef718b132cee0c4260278337a4d56", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 108550, "stop": 110157, "observations": { "mutation": [ 108559, 108604, 108766, 108778, 110128 ], "stop": [ 108559, 108604, 108766, 108778, 110128 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDZ7", "query_cov": 1.0, "subject_cov": 0.6016949152542372, "identity": 1.0, "score": 684.0, "evalue": 1.41e-241, "gene_start": 1, "gene_end": 426, "reference_start": 274, "reference_end": 699, "reference_sequence": "MIINTKLDINIYIKMYIINVMKIEVNSVRKRLKIFAREKKNENDVVGDVSYSIPGEILKFLILLFVALAFIMFQYQDLFFHNDNKVINIIRKIYLYSFGLAFGDIITLVIIGSFLTLMVRWFGSIFKNHYFAWFKSYTRVDYWILRKRIISFVWLTLLFVVLIYHVVLVSFRVENIWYYSTADVKNIYLKGWYYTFTNQADLLDGKSVDYPVAYLNIGFLLDTLFNLFYVVTFSPYLAIISAFLIWTLAWLFLVTLNPIKYFKNINEHKKTIVMVEQYLQKINSLFYYTPQVRELFRFYKDAAQVLKVDYFKFNFRYLQKLVKKNLTLLAQHTLTAKYFENVKAKKTASQLEAEEFLKIVETTQTNTTSATITLNAAADIKMPAINAQNLLVNKHNKNNTNTYISNESTSEYSKDFDLTEERYMSNYVETIEVEAQSQESTKLLIDDLENKKSDTEEIKAKTKEMTAKFEAVQPTEEKTKEITNILLADQVISEVTKEEKLAPTIEQVSQQFLVDTNELTDTNDVEKDQTQSNIIDQNNSKTVKLVKETIENEESKTLEQEIAFLIDSSENTFVMSAEKNTQNITQTMSQPVEVKAQTIEQKIEENVENKKQTIGFIIPEDSNETIIEKDDDLKTKEEFIAQLDSIEADNLASKEIKLEQPESEKRQNFQFTFDTNTIVINNDSNKSKDRVDHFEDDNDWVSPILSDN" }, "paralog": false, "identity": 0.9906542056074766, "score": 932.0, "evalue": 0.0, "description": "Internal stop codon at 108559, 108604, 108766, 108778, 110128. Nonsense mutation around 108559, 108604, 108766, 108778, 110128.", "psc": { "uniref90_id": "UniRef90_C4XDZ7", "uniref50_id": "UniRef50_C4XDZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDZ7", "UniRef:UniRef50_C4XDZ7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XDZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XDZ7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 49151.044900000095, "isoelectric_point": 4.635867881774902 }, "id": "OAIKOHHJFB_235", "locus": "GCA000209735_00203" }, { "type": "cds", "sequence": "contig_1", "start": 110277, "stop": 110390, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACCAATTGTTGAAAGTGTTGAAGAAGAATATAAAGAATACAATGAAATTATTTTTCTAGAAGTTGATGCTGAAAAAGCTCAATTATTTAGAGAAATCAATAACCGTTGA", "aa": "MKPIVESVEEEYKEYNEIIFLEVDAEKAQLFREINNR", "aa_hexdigest": "d035d9e1e33c59dcdacd73cadc0fb5e4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4517.0309, "isoelectric_point": 4.431986427307129 }, "id": "OAIKOHHJFB_236", "locus": "GCA000209735_00204" }, { "type": "cds", "sequence": "contig_1", "start": 110634, "stop": 111017, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCACCAATATTTTGAAGGTGCAATTAACTCATATAAAACAAGTAGGCTTGTTGAAGAAATAAGAGCTTACGAAGTTTTTATTTGCAAAACCAATAAAGAAGCATTATTACTTGAAAAACTTTACATTGATAAATATAATCCTGAATATAATATTTTGCTTTTGGATGACCGTCGTTATCCATATTTAAAAGTACAATTAAGTGACAGGGGTTTAGATATTTCATTGTCAAGAAAAATAGGCAAAAAGAATGAAACTAATAAATTATTTTACTTTGGACCTTTTCCAATAGGTTATGGTTCTATTCAAATATTGAAATTATTAGAACGTGAAGCATTTTATGAAAAAGGTTTAAAAATCAAAAATAATGATCCTAAATTTTGA", "aa": "MHQYFEGAINSYKTSRLVEEIRAYEVFICKTNKEALLLEKLYIDKYNPEYNILLLDDRRYPYLKVQLSDRGLDISLSRKIGKKNETNKLFYFGPFPIGYGSIQILKLLEREAFYEKGLKIKNNDPKF", "aa_hexdigest": "fee39558eb5b77120edeffab94938226", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15099.314499999984, "isoelectric_point": 9.176897239685058 }, "id": "OAIKOHHJFB_237", "locus": "GCA000209735_00205" }, { "type": "cds", "sequence": "contig_1", "start": 111252, "stop": 112163, "strand": "+", "frame": 3, "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "db_xrefs": [ "GO:0006289", "GO:0009380", "GO:0009381", "KEGG:K03703", "SO:0001217", "UniRef:UniRef50_O84898", "UniRef:UniRef90_C4XDZ9" ], "nt": "ATGACAATCCTTTTTTATCGTTCAGGTCTATTAATTAATAAAGATAATCAAGCTTTAAATATTGACATTGATGAAATTGAAACACTTGAAAAATTCTTTAATAAATATTATGAAAATAAAATAAAGCCAGATGAAATTTTAGTTGAAGAAAAAATTAACAATTTAAACTTAAATTTAGATGAAAATTTAAATATAGTTACACCTAAAATAGGTGATAAAAAACGTGTTTTAGAAATAGCACAATTAAACCTTGAAGAGTATTTTAGTAAAGAACATTTACTAATTAAAAATAAAAATGATCATGCATTATTAATGCTTGAAGAATTTAAAAAGCTAATCAAAATTGATTCATTAAAAAATATCATTTTATTTGATAACTCAAATTTAGCTAATTCTAATCCTGTCGGTGTTGCTATAGTTTATTCTAATGGTTTAAAAAACAAAAATTTATACAGAAAATTTAATCACATTGATATTTATTCTAGAGAAGCAGATGTTGAATACATGAAAGAATCAATTAAAAAATACTTTAGTTCAGCAGACAACAAAAAAGATTTTGATTTAATTATTGTTGATGGTGGTTTAGCTCAAATACATGAAGCTAAAAAAGTTTTGTTTGATTTAGATATTAAAATACCAATTGTTGGTTTAGTTAAAGATGATTTTCATAGAACAAGAGCTTTAATTGATTTACAAGAAAATTTAGTTAAAATAAAAAATAAAGATTTACTAAACTTTTTGGCAAGTATGCAAATTGAAGTCGATCGCTTTGCTAAAAGCCATTTGAGAAATAGAAATCGTATCACTACTTTTGAAGGCAGCTTACAATCAATTAAAGGTTTAGGTAAATGTGCGGAAACTAAATTACTAAATACATTAAAACATATGTTAATATCTTTAACGCTCCTTTAG", "aa": "MTILFYRSGLLINKDNQALNIDIDEIETLEKFFNKYYENKIKPDEILVEEKINNLNLNLDENLNIVTPKIGDKKRVLEIAQLNLEEYFSKEHLLIKNKNDHALLMLEEFKKLIKIDSLKNIILFDNSNLANSNPVGVAIVYSNGLKNKNLYRKFNHIDIYSREADVEYMKESIKKYFSSADNKKDFDLIIVDGGLAQIHEAKKVLFDLDIKIPIVGLVKDDFHRTRALIDLQENLVKIKNKDLLNFLASMQIEVDRFAKSHLRNRNRITTFEGSLQSIKGLGKCAETKLLNTLKHMLISLTLL", "aa_hexdigest": "4322776c6a9a76647afa13a6a9e22a47", "start_type": "ATG", "rbs_motif": null, "genes": [ "uvrC" ], "truncated": "both", "pseudogene": { "start": 110952, "stop": 112234, "observations": { "deletion": [ 112128 ], "mutation": [ 111015, 111150 ], "stop": [ 111015, 111150 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XDZ9", "query_cov": 0.9702970297029703, "subject_cov": 0.5121951219512195, "identity": 0.997, "score": 563.0, "evalue": 5.08e-198, "gene_start": 1, "gene_end": 294, "reference_start": 248, "reference_end": 541, "reference_sequence": "MTKEEIITSLKNVSTAPGIYLWKDKNDQVIYVGKAKNLRKRMHQYFEGAINSYKTSRLVEEIRAYEVFICKTNKEALLLEKLYIDKYNPEYNILLLDDRRYPYLKVQLSDRGLDISLSRKIGKKNETNKLFYFGPFPIGYGSIQILKLLEREAFYEKGLKIKNNDPKFWQEKFNKIKSILSFKDNSYINEIKEKMFKEAETEHFELARDLKLSWEYLKKLNEEQIIELKDIHNLDVFAYKTVDNIIYMTILFYRSGLLINKDNQALNIDIDEIETLEKFFNKYYENKIKPDEILVEEKINNLNLNLDENLNIVTPKIGDKKRVLEIAQLNLEEYFSKEHLLIKNKNDHALLMLEEFKKLIKIDSLKNIILFDNSNLANSNPVGVAIVYSNGLKNKNLYRKFNHIDIYSREADVEYMKESIKKYFSSADNKKDFDLIIVDGGLAQIHEAKKVLFDLDIKIPIVGLVKDDFHRTRALIDLQENLVKIKNKDLLNFLASMQIEVDRFAKSHLRNRNRITTFEGSLQSIKGLGKCAETKLLNTFKTYVNIFNAPLEELEKVVSKKIALEIKKLYESKK" }, "paralog": false, "identity": 0.9929742388758782, "score": 818.0, "evalue": 4.91e-301, "description": "Internal stop codon at 111015, 111150. Frameshift due to deletion around 112128. Nonsense mutation around 111015, 111150.", "psc": { "uniref90_id": "UniRef90_C4XDZ9", "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "uniref50_id": "UniRef50_O84898", "kegg_orthology_id": "K03703", "go_ids": [ "GO:0006289", "GO:0009380", "GO:0009381" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XDZ9", "UniRef:UniRef50_O84898", "KEGG:K03703", "GO:0006289", "GO:0009380", "GO:0009381" ] }, "pscc": { "uniref50_id": "UniRef50_O84898", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O84898" ], "product": "UvrABC system protein C" } }, "id": "OAIKOHHJFB_238", "locus": "GCA000209735_00206" }, { "type": "cds", "sequence": "contig_1", "start": 112630, "stop": 113055, "strand": "+", "frame": 1, "gene": null, "product": "6-phospho-beta-glucosidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE01", "UniRef:UniRef90_C4XE01" ], "nt": "TTGTTCAAAGAATTAAAGAGAAGAAAAATAAAAACAATAGTTACTCTTTGTCATTATGAAATGCCTTTACATTTAGCACTTAAATACAATGGTTTTAATAATAAAAAAGTTGTTAAATACTTTTTAAATTATGCTAAAGTAGTTCTAAAATACTTTAATAAATACATAGATTATTTAGTAGCTTTTAATGAAATTAATTCACTTTTACATATTCCTTTTTGTAATGGCGGAATTTACAAAAATCAAGAAGATTTAAATATTTTATATCAAGCAACTCATAATATGCTTTATGCTTATGCTAAAGTCAAAGAAATAATTAAAAATAATTACAATCAGATTCAAGTAGGTTCAATGGTATTAGCTATGCCTTTTTATACTAAAAGTGCAAAAGTAGAAGATCAAATATTGAAAATGGAAAAAGAATGA", "aa": "MFKELKRRKIKTIVTLCHYEMPLHLALKYNGFNNKKVVKYFLNYAKVVLKYFNKYIDYLVAFNEINSLLHIPFCNGGIYKNQEDLNILYQATHNMLYAYAKVKEIIKNNYNQIQVGSMVLAMPFYTKSAKVEDQILKMEKE", "aa_hexdigest": "31ca9bdaaede8a489d8725233dc27a84", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 112528, "stop": 113213, "observations": { "deletion": [ 113125 ], "mutation": [ 112561, 113053 ], "stop": [ 112561, 113053 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE01", "query_cov": 1.0, "subject_cov": 0.618421052631579, "identity": 0.9790000000000001, "score": 278.0, "evalue": 1.71e-93, "gene_start": 1, "gene_end": 141, "reference_start": 35, "reference_end": 175, "reference_sequence": "MGIKVLRISIAWSRIFPKGDESKPNQKGLEFYLKLFKELKRRKIKTIVTLCHYEMPLHLALKYNGFSNKKVVKYFLNYAKVVLKYFNKYIDYLVAFNEINSLLHIPFCNGGIYKNQEDLNILYQATHNMLYASAKVKEIIKNNYNQIQVGSMVLAMPFYTKSAKVEDQILKMEKEWQNLYFTDVLARGVYPKYLDNYFLKNNIKINKTKEELEVIKNNIVDYIGFSYC" }, "paralog": false, "identity": 0.9824561403508771, "score": 444.0, "evalue": 1.68e-161, "description": "Internal stop codon at 112561, 113053. Frameshift due to deletion around 113125. Nonsense mutation around 112561, 113053.", "psc": { "uniref90_id": "UniRef90_C4XE01", "product": "6-phospho-beta-glucosidase", "uniref50_id": "UniRef50_C4XE01", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE01", "UniRef:UniRef50_C4XE01" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE01", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE01" ], "product": "6-phospho-beta-glucosidase" } }, "id": "OAIKOHHJFB_239", "locus": "GCA000209735_00207" }, { "type": "cds", "sequence": "contig_1", "start": 113315, "stop": 113416, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCF1", "UniRef:UniRef90_A0AB32XCF1" ], "nt": "ATGTTAAGCAGAGAATATTACACCTTTAATAATATAAATTGCATTAAAGATTACACAAAAAACATTCATTTAATCATTTTAGATGATCCAGATAGTGAATGA", "aa": "MLSREYYTFNNINCIKDYTKNIHLIILDDPDSE", "aa_hexdigest": "bf6438b1ab969c940bd1670c9fe5b05a", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 113315, "stop": 113482, "observations": { "mutation": [ 113414 ], "stop": [ 113414 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XCF1", "query_cov": 1.0, "subject_cov": 0.6, "identity": 1.0, "score": 70.9, "evalue": 1.2e-15, "gene_start": 1, "gene_end": 33, "reference_start": 1, "reference_end": 33, "reference_sequence": "MLSREYYTFNNINCIKDYTKNIHLIILDDPDSEWISNNYFRNKVHIIFKSEGGKF" }, "paralog": false, "identity": 0.9818181818181818, "score": 115.0, "evalue": 1.06e-35, "description": "Internal stop codon at 113414. Nonsense mutation around 113414.", "psc": { "uniref90_id": "UniRef90_A0AB32XCF1", "uniref50_id": "UniRef50_A0AB32XCF1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XCF1", "UniRef:UniRef50_A0AB32XCF1" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB32XCF1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCF1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 3992.4436000000005, "isoelectric_point": 4.676394081115721 }, "id": "OAIKOHHJFB_240", "locus": "GCA000209735_00208" }, { "type": "cds", "sequence": "contig_1", "start": 113552, "stop": 113704, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACTTGTTGTTAACTTTATTTTAGTTTGCTTAATATATTTAATTGGATTGTTTCTATTTCCTCTCACTTATTTCAAATGTAAAAACTATGAAACAGATTTAAAAAACAATGTTAAGTTCAATGTAGACTTATATGGAGAAGCTAAATAA", "aa": "MKLVVNFILVCLIYLIGLFLFPLTYFKCKNYETDLKNNVKFNVDLYGEAK", "aa_hexdigest": "70377fa20cdf25e2d7eb34176df54173", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5910.058200000002, "isoelectric_point": 8.638263893127444 }, "id": "OAIKOHHJFB_241", "locus": "GCA000209735_00209" }, { "type": "cds", "sequence": "contig_1", "start": 113796, "stop": 114062, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTACTCTTTCATCATTAAAGGTCGCTTTAAAGATAAAGCTTTTAAGCACTAAAGCAAAAATTAAAGGAATAATAATAATTGCAGCATATATCACTATTTGGGTAGTGCTAACAAATGTATTTGCATTGGTTGTAGTATCACGATTCCGTAAATTGAGTCATGATGCTAATGCGCCTATACCGATTGTTGCAATATTAGCAGCAAAAGCAATTCAAGATAATTTTTTTATCTTATCAATAATAGATAATTGTTCTTCCATATGA", "aa": "MITLSSLKVALKIKLLSTKAKIKGIIIIAAYITIWVVLTNVFALVVVSRFRKLSHDANAPIPIVAILAAKAIQDNFFILSIIDNCSSI", "aa_hexdigest": "7af78ef7a1ecb8640d0d73e7f2e97d05", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9577.599199999993, "isoelectric_point": 10.106337928771971 }, "id": "OAIKOHHJFB_242", "locus": "GCA000209735_00210" }, { "type": "cds", "sequence": "contig_1", "start": 114432, "stop": 114923, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13635.12" ], "nt": "ATGCCTGAAGTAGTTTTAGTAGATAATGAAAAAGAAAAGAAACAAATATTAAAAGATCTTTTAACAAATGTTTATGTTAATGACATTGTTGAGAGACATAAACTTAATAGTACTCAAGATGTTTTAAACATAGTTGAATATCTTGCTTCAATAGTTTCATCTCAATGTAATTCAAGTAGAATTGCCAACTCTTTTAATTCTTTAAATAATAAAAAGACTTCTACTTATCAAATAGATAAAATATTAGATGCACTAGAAGATTCTTTCTTAATAGATAAAATAAGAAAATATGATATTAAAGGTTTAAAACTTCTTTCGGGTCAATATAAATACTATTTTGTTGACAACGGATTAAGAAATGTAGCTCTCAATTTTAGAAATCTAGGACAAGGTCAATTAATCGAAAACGCCTTTTTTAATGCTCTAAAAATAAATTACTTCGATATTAGTTATGGGGCAATTAATCAAAAAAATAATATTAATAATCAATGA", "aa": "MPEVVLVDNEKEKKQILKDLLTNVYVNDIVERHKLNSTQDVLNIVEYLASIVSSQCNSSRIANSFNSLNNKKTSTYQIDKILDALEDSFLIDKIRKYDIKGLKLLSGQYKYYFVDNGLRNVALNFRNLGQGQLIENAFFNALKINYFDISYGAINQKNNINNQ", "aa_hexdigest": "d1f872c9c0f9a504df55fe1e5a861e93", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13635.12", "name": "DUF4143", "length": 127, "aa_cov": 0.7361963190184049, "hmm_cov": 0.952755905511811, "evalue": 1.4567564089435346e-13, "score": 46.9279670715332, "start": 27, "stop": 146 } ], "seq_stats": { "molecular_weight": 18749.07219999999, "isoelectric_point": 8.526024436950681 }, "id": "OAIKOHHJFB_243", "locus": "GCA000209735_00211" }, { "type": "cds", "sequence": "contig_1", "start": 114948, "stop": 115130, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTTTAAATTCATTTCAAGATCAAGATAATATTGATATTTATGTTACTGGCAGTAATTCTAAATTCTTATNCAAAGATATAGCTACTACTTTTGCAGGTCGAGGTATGGAGATTAATGTTTTGCCTTTATCATTTCAAGAATATTTAAACTTTAGTAATCTTGATATAAATGAAGCTTGA", "aa": "MVLNSFQDQDNIDIYVTGSNSKFLXKDIATTFAGRGMEINVLPLSFQEYLNFSNLDINEA", "aa_hexdigest": "9148b757c54c8f414d438434ab2e1e41", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": null, "isoelectric_point": 4.0500284194946286 }, "id": "OAIKOHHJFB_244", "locus": "GCA000209735_00212" }, { "type": "gap", "sequence": "contig_1", "start": 115057, "stop": 115057, "strand": ".", "length": 1, "id": "OAIKOHHJFB_1794" }, { "type": "cds", "sequence": "contig_1", "start": 115230, "stop": 115439, "strand": "-", "frame": 3, "gene": null, "product": "AAA domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4Y8VNC6" ], "nt": "ATGAATAAAGTTATTAAAAGAGACAATTATTTAAATATGCTAATTAATAAAATCAATAACAAAAAAATTAAAATAATTACAGGTATAAGAAGAGTAGGCAAAAGTTTTTTACTCTTCAATTTATTCTATAGTTATTTATTAGCTAATAAGGTGGAAGCTGACCAAATAATAACAATAAAACTTGACCGAGTTGAAAATGAAGAACTTTGA", "aa": "MNKVIKRDNYLNMLINKINNKKIKIITGIRRVGKSFLLFNLFYSYLLANKVEADQIITIKLDRVENEEL", "aa_hexdigest": "178371902963c983f955048731aec3f6", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI002FEFEF55", "query_cov": 0.9420289855072463, "subject_cov": 0.9154929577464789, "identity": 0.615, "score": 80.5, "evalue": 1.28e-18, "valid": false, "product": "AAA family ATPase", "uniref50_id": "UniRef50_A0A4Y8VNC6" }, "pscc": { "uniref50_id": "UniRef50_A0A4Y8VNC6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4Y8VNC6" ], "product": "AAA domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_245", "locus": "GCA000209735_00213" }, { "type": "cds", "sequence": "contig_1", "start": 115697, "stop": 115888, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCV5", "UniRef:UniRef50_UPI000FF82D94", "UniRef:UniRef90_A0AB32XCV5", "UniRef:UniRef90_UPI000FF82D94" ], "nt": "ATGATTAAAATTGCTGAATTGTTAAAAGTTCAAATAGAAGAAGTTAGAAACAATTATCAAAACTTTTTATGTTTGTTTAGCGAAGCGAGACTAAGAGTTAGATTGTGTAGAAAAGAAAACATGCATTTTGGCGATGAATTACAAATAGACGCTTGTATATATGAATCATTTGATAGGACTATGAGTGCATAA", "aa": "MIKIAELLKVQIEEVRNNYQNFLCLFSEARLRVRLCRKENMHFGDELQIDACIYESFDRTMSA", "aa_hexdigest": "5f33095ddcfbf7a9d2333b8e8e03b991", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000FF82D94", "query_cov": 1.0, "subject_cov": 0.9545454545454546, "identity": 0.9840000000000001, "score": 127.0, "evalue": 3e-37, "valid": true, "uniref50_id": "UniRef50_UPI000FF82D94" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF82D94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF82D94" ] }, "genes": [], "truncated": "both", "pseudogene": { "start": 115688, "stop": 116107, "observations": { "mutation": [ 115699, 116029 ], "stop": [ 115699, 116029 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XCV5", "query_cov": 1.0, "subject_cov": 0.45323741007194246, "identity": 1.0, "score": 129.0, "evalue": 2.2e-37, "gene_start": 1, "gene_end": 63, "reference_start": 74, "reference_end": 136, "reference_sequence": "MEEYTEYIKNKINGIKNVNDATIKSFWKEIRIVDYSHVGYEKQISYQTLVNRLTSLGFLTIFSHKKTRRKVRKMIKIAELLKVQIEEVRNNYQNFLCLFSEARLRVRLCRKENMHFGDELQIDACIYESFDRTMSAYYH" }, "paralog": false, "identity": 0.9856115107913669, "score": 273.0, "evalue": 2.87e-96, "description": "Internal stop codon at 115699, 116029. Nonsense mutation around 115699, 116029.", "psc": { "uniref90_id": "UniRef90_A0AB32XCV5", "uniref50_id": "UniRef50_A0AB32XCV5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XCV5", "UniRef:UniRef50_A0AB32XCV5" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB32XCV5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCV5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7543.685099999997, "isoelectric_point": 5.732689857482911 }, "id": "OAIKOHHJFB_246", "locus": "GCA000209735_00214" }, { "type": "cds", "sequence": "contig_1", "start": 116484, "stop": 116744, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTTTTATTGATTATTTTTTAGATTGCTTAATTATTTCCTACAATAAATTAATAGATGTAAATAAAAAAGATTATTTAGAAAATCAAATAATCGAAATTTTAACTACTAGCTTAATTCCTTTATCAACTAAAGAAATAAGTGAACGACTTGTTTCAATTTCAAGAAGAACATTAGAAAGAAAACTTAAAGTCTTATTAGAACAAAATAAAATCAAAAAAGTTGGTCAATATAAAAATGCTAAATATAAATTAACATAA", "aa": "MPFIDYFLDCLIISYNKLIDVNKKDYLENQIIEILTTSLIPLSTKEISERLVSISRRTLERKLKVLLEQNKIKKVGQYKNAKYKLT", "aa_hexdigest": "3ebfb0117e7a39f810d8dc0f7f8bffd5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10177.961699999987, "isoelectric_point": 9.519095420837402 }, "id": "OAIKOHHJFB_247", "locus": "GCA000209735_00215" }, { "type": "cds", "sequence": "contig_1", "start": 116766, "stop": 117530, "strand": "-", "frame": 3, "gene": null, "product": "Fido domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE05", "UniRef:UniRef90_C4XE05" ], "nt": "ATGGTTAAAAAAGAAAAAAGGTTTTTTGATTATTCATTTTTAAAGAACATTATTAATAATGAAATTAACAAAAAATTAAAACAAATTTATGAGTTAAATGGACTTATAAATGATTTTTCTAATATTCCAGAACAAACTAAAAATACTTTAATTAAAAATAGTATTTTTCAAAGTGTTACTTCTTCAAACAAAATTGAAAATATTGTTTCAACTACAAACAAAATTGAAAATATTATTTTTAATAATAAAAAATATACAGGCGAAAACGAATTAGAAATTCAAGGTTATAATGATGTATTAAATATTATTTTAAATAATTTTGATAATATAGATATTACTAAATCAAACATCTTAAATTTACATAATCAATTATATAAATATAGTAATAAAACTTATTTAAAAGGAAAGTTTAAAGAAATGAACAATTATATTTTTTCAATTGACCAAAACAACTATAAAACTGTTATATTTGAACCGGTTGATCATTCTTTGGTTGATGATTACATAACAAAAATATGTGATGAATACAATAAAAGAAATGATGAAAAAATAATTGATCCTTTAATACTAATTCCAACATTTATTTTGGATTTTTTATGTATTCACCCTTTTAGTGATGGCAATGGTAGAATTTCAAGATTATTAACTAACTGTTTATTATTTCAAAATGATTTTATGATTAATAAATACATTAGTTTTGAAAAAATCATAGAAGAAAATTTAAATCAATATTATTTCACTTTAAGAAAAAGCTCAACTAATTGA", "aa": "MVKKEKRFFDYSFLKNIINNEINKKLKQIYELNGLINDFSNIPEQTKNTLIKNSIFQSVTSSNKIENIVSTTNKIENIIFNNKKYTGENELEIQGYNDVLNIILNNFDNIDITKSNILNLHNQLYKYSNKTYLKGKFKEMNNYIFSIDQNNYKTVIFEPVDHSLVDDYITKICDEYNKRNDEKIIDPLILIPTFILDFLCIHPFSDGNGRISRLLTNCLLFQNDFMINKYISFEKIIEENLNQYYFTLRKSSTN", "aa_hexdigest": "ef0bf0dce3874e5ff86f93460df0962a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 116484, "stop": 117539, "observations": { "mutation": [ 116768 ], "stop": [ 116768 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE05", "query_cov": 1.0, "subject_cov": 0.7236467236467237, "identity": 1.0, "score": 472.0, "evalue": 2.27e-166, "gene_start": 1, "gene_end": 254, "reference_start": 4, "reference_end": 257, "reference_sequence": "MIIMVKKEKRFFDYSFLKNIINNEINKKLKQIYELNGLINDFSNIPEQTKNTLIKNSIFQSVTSSNKIENIVSTTNKIENIIFNNKKYTGENELEIQGYNDVLNIILNNFDNIDITKSNILNLHNQLYKYSNKTYLKGKFKEMNNYIFSIDQNNYKTVIFEPVDHSLVDDYITKICDEYNKRNDEKIIDPLILIPTFILDFLCIHPFSDGNGRISRLLTNCLLFQNDFMINKYISFEKIIEENLNQYYFTLRKSSTNWQYSQNDYLPFIDYFLDCLIISYNKLIDVNKKDYLENQIIEILTTSLIPLSTKEISERLVSISRRTLERKLKVLLEQNKIKKVGQYKNAKYKLT" }, "paralog": false, "identity": 0.9943019943019943, "score": 669.0, "evalue": 2.1e-246, "description": "Internal stop codon at 116768. Nonsense mutation around 116768.", "psc": { "uniref90_id": "UniRef90_C4XE05", "product": "Fido domain-containing protein", "uniref50_id": "UniRef50_C4XE05", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE05", "UniRef:UniRef50_C4XE05" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE05", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE05" ], "product": "Fido domain-containing protein" } }, "id": "OAIKOHHJFB_248", "locus": "GCA000209735_00216" }, { "type": "cds", "sequence": "contig_1", "start": 117945, "stop": 118802, "strand": "-", "frame": 3, "gene": null, "product": "ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_R9RA50", "UniRef:UniRef90_UPI000FEE809E" ], "nt": "ATGCTTAAAAGCGCAGGTTTAATTCTAAAAGATCCTGAAACCAATAAAGAAGGAATAACTATAGCAGCTATTTTGCTTTTTGGAAAAGATCAAACTATTATGTCTGTTCTTCCTCAATATCGTACAGATGCTATTTTTAGAGTTAAAAATAAAGATAGATATGATGATAGAGAAATAGTAGCAACCAATTTAATCGATAGCTATGAGAAATTAATTAATTTTGGTGAAAAACACTTAAGCGATATTTTTGTTTTAGATGGTATTGTAAGTGTTAGTGCTCGTAATTTGATACTTAGAGAAATCATTTCTAATACACTAGCTCATAGAAAATATGCAAGCAATATGACAGCTACTATTGTCATTGATGAAAACAATATAACTGTTGAAAATGATAATAGTTCAAGCAGTAAAGAGGAATTAGATCCTAAAACCTTTAGACCAAATCCAAAAAATCCACCAATTTCAAAAGTTTTTAGAGAAATTGGTTTAGCTGATGAATTAGGTTCAGGAATTAGAAAAACTTATAAATATACAAAACTTTATTCAAATAATAAACCTACATTTTTGGATAGTAACATATTTAAAACGATTATTCCTATTCCAAAAATTGCTACATTAAAAGTTGGAAAAATGACCTCTAATATAGATACAGATAATAAAAATATTACAAACAAAAAACAATTAAATATTGAAAGAATTTTCAATTTAGTTAAAGAAAATTCTACAATAAAAAGAAGTGAAATTGCAGCAATTTTAAATTTAACTGAAAAAACTATTTCAAGATATATTAGAGAAATAAAAAACATAAAATATGTTGGAAAAGGTAAAAATGGTTATTGGGAAATTATTGATAAATAG", "aa": "MLKSAGLILKDPETNKEGITIAAILLFGKDQTIMSVLPQYRTDAIFRVKNKDRYDDREIVATNLIDSYEKLINFGEKHLSDIFVLDGIVSVSARNLILREIISNTLAHRKYASNMTATIVIDENNITVENDNSSSSKEELDPKTFRPNPKNPPISKVFREIGLADELGSGIRKTYKYTKLYSNNKPTFLDSNIFKTIIPIPKIATLKVGKMTSNIDTDNKNITNKKQLNIERIFNLVKENSTIKRSEIAAILNLTEKTISRYIREIKNIKYVGKGKNGYWEIIDK", "aa_hexdigest": "247aaeb9d07c8ab815028e24a9562fe0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000FEE809E", "query_cov": 1.0, "subject_cov": 0.8431952662721893, "identity": 0.996, "score": 541.0, "evalue": 1.91e-193, "valid": true, "product": "ATP-binding protein", "uniref50_id": "UniRef50_R9RA50" }, "pscc": { "uniref50_id": "UniRef50_R9RA50", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_R9RA50" ], "product": "Helix-turn-helix type 11 domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_249", "locus": "GCA000209735_00217" }, { "type": "cds", "sequence": "contig_1", "start": 119031, "stop": 119366, "strand": "-", "frame": 3, "gene": null, "product": "helix-turn-helix domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF015A0", "UniRef:UniRef90_UPI000FF015A0" ], "nt": "ATGAATAAAGAAGAATTGAAAAAACTTTTAATCCAAGGTGAAGGCGAAGAAATAGAATTCAAAACATGCAAAGATGCCTTATCTAAAAGTGTTTTTGATACCGTTTCAGCTTTTAGCAATAAAAATGGAGGTCATCTAATTTTAGGTATTGGAGACAATGGTGAAGTTTTAGGTGTAGACAAAACTAAAGTTAAAAAAATAGTAAATGACTTCTTGAATTCAATTCATGATTCAACTAAAATTAATCCTCCTTTATTTATTGAACCTGAAGTAATAGATTTTGATGATAAAAAAATAATTTATATTTATGTTCCAGTTGGAGTAAATGTTTGTTGA", "aa": "MNKEELKKLLIQGEGEEIEFKTCKDALSKSVFDTVSAFSNKNGGHLILGIGDNGEVLGVDKTKVKKIVNDFLNSIHDSTKINPPLFIEPEVIDFDDKKIIYIYVPVGVNVC", "aa_hexdigest": "3b9ecac3630c9d99ab28510e6fa7eb9a", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 118922, "stop": 119366, "observations": { "insertion": [ 118943 ], "mutation": [ 118961, 119033 ], "stop": [ 118961, 119033 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000FF015A0", "query_cov": 1.0, "subject_cov": 0.7551020408163265, "identity": 1.0, "score": 217.0, "evalue": 3.89e-71, "gene_start": 1, "gene_end": 111, "reference_start": 1, "reference_end": 111, "reference_sequence": "MNKEELKKLLIQGEGEEIEFKTCKDALSKSVFDTVSAFSNKNGGHLILGIGDNGEVLGVDKTKVKKIVNDFLNSIHDSTKINPPLFIEPEVIDFDDKKIIYIYVPVGVNVCWHNGRIFERSFEGDVDITNKDELVWKLFQKKKVFVL" }, "paralog": false, "identity": 0.9863945578231292, "score": 283.0, "evalue": 1.68e-99, "description": "Internal stop codon at 118961, 119033. Frameshift due to insertion around 118943. Nonsense mutation around 118961, 119033.", "psc": { "uniref90_id": "UniRef90_UPI000FF015A0", "product": "helix-turn-helix domain-containing protein", "uniref50_id": "UniRef50_UPI000FF015A0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000FF015A0", "UniRef:UniRef50_UPI000FF015A0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000FF015A0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF015A0" ], "product": "helix-turn-helix domain-containing protein" } }, "id": "OAIKOHHJFB_250", "locus": "GCA000209735_00218" }, { "type": "cds", "sequence": "contig_1", "start": 119940, "stop": 120215, "strand": "+", "frame": 3, "gene": null, "product": "MAGa4850 family ICE element protein", "db_xrefs": [ "RefSeq:WP_258408915.1", "SO:0001217", "UniParc:UPI0021AD3CCC", "UniRef:UniRef100_C4XEU5", "UniRef:UniRef50_C4XF94", "UniRef:UniRef90_C4XF94" ], "nt": "ATGAGTGTATACGGCGAACTAAGGTTAATTGCAAATGAAGGAAGCGAAAACGAAGTTAGAAAGTTTGAAGCTAACTTGGAATGGAGAAAAAATTATTTTGGCAAGAAGGTTTATGACAAGTTAAGCCAAGATACAGTTAGTGAAATTTTAAAAACAAAGATAGTACTCGGTGAAAGCTACTATAAGATTTTGAGAACTGAAAAAGTTGCTTTTGAAGTGTATGTCTGTTTAAGGTTTCTTGGGGCTAAAAAACGCTATGTAAATTTTTATGAATAG", "aa": "MSVYGELRLIANEGSENEVRKFEANLEWRKNYFGKKVYDKLSQDTVSEILKTKIVLGESYYKILRTEKVAFEVYVCLRFLGAKKRYVNFYE", "aa_hexdigest": "f16727b976cacd1413f78d7ab6ad0433", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0021AD3CCC", "ncbi_nrp_id": "WP_258408915.1", "uniref100_id": "UniRef100_C4XEU5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0021AD3CCC", "RefSeq:WP_258408915.1", "UniRef:UniRef100_C4XEU5" ] }, "ips": { "uniref100_id": "UniRef100_C4XEU5", "uniref90_id": "UniRef90_C4XF94" }, "psc": { "uniref90_id": "UniRef90_C4XF94", "product": "MAGa4850 family ICE element protein", "uniref50_id": "UniRef50_C4XF94" }, "pscc": { "uniref50_id": "UniRef50_C4XF94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF94" ] }, "genes": [], "id": "OAIKOHHJFB_251", "locus": "GCA000209735_00219" }, { "type": "gap", "sequence": "contig_1", "start": 120342, "stop": 120441, "strand": ".", "length": 100, "id": "OAIKOHHJFB_1795" }, { "type": "cds", "sequence": "contig_1", "start": 120496, "stop": 120906, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_258408916.1", "SO:0001217", "UniParc:UPI0002F51F6A", "UniRef:UniRef100_UPI0002F51F6A", "UniRef:UniRef50_C4XE08", "UniRef:UniRef90_C4XE08" ], "nt": "TTGAAGAAATTACCTGCTCTTGCATTAAAAGGGTTCTTATGTCTTCAAGCATATTTAGTGGGCGGTTTGCCTTTAGTAATAGGTATAACTGCTATTAAATATTTCTTTGATAAAAGAAAAGAAAAAGCTTTAGCTAAAAATGAGATAAAGCCGGAAATTGCAAAGAACTTTAAGTTGAGCGATGGTGTTGCTAAAGAGATAACAAAAACGTTTAATGAAGCAATGAAAGAAATTGTTGTTTCAACTAAAGAGGCTGTGATGAGCCAATACCAAAAGGATTTAGCAAGTGGAAAGATTATAGCTGACGCATATGATAATGCGAATATTGAAAAAGCACAAAATGCAGCTATGAATAAAGAAATTGCTAAAGAAACAAAAATTGAAAATCCAAAGTCAAAGGCTAAAAAATAG", "aa": "MKKLPALALKGFLCLQAYLVGGLPLVIGITAIKYFFDKRKEKALAKNEIKPEIAKNFKLSDGVAKEITKTFNEAMKEIVVSTKEAVMSQYQKDLASGKIIADAYDNANIEKAQNAAMNKEIAKETKIENPKSKAKK", "aa_hexdigest": "e13302b49fa5df8924127e6d23a420ac", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0002F51F6A", "ncbi_nrp_id": "WP_258408916.1", "uniref100_id": "UniRef100_UPI0002F51F6A", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002F51F6A", "RefSeq:WP_258408916.1", "UniRef:UniRef100_UPI0002F51F6A" ] }, "ips": { "uniref100_id": "UniRef100_UPI0002F51F6A", "uniref90_id": "UniRef90_C4XE08" }, "psc": { "uniref90_id": "UniRef90_C4XE08", "uniref50_id": "UniRef50_C4XE08" }, "pscc": { "uniref50_id": "UniRef50_C4XE08", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE08" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15035.591299999985, "isoelectric_point": 9.595812797546387 }, "id": "OAIKOHHJFB_252", "locus": "GCA000209735_00220" }, { "type": "cds", "sequence": "contig_1", "start": 120915, "stop": 121103, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013526823.1", "SO:0001217", "UniParc:UPI00000C02A5", "UniRef:UniRef100_C4XE09", "UniRef:UniRef50_C4XE09", "UniRef:UniRef90_C4XE09" ], "nt": "ATGGAAAAGAAGAAAGGAAAAGGAAAAATTATGCATGAGATTAAAAACAATAAATTTGATGAAATTTTATTAAGTGAAGAATCAAAACAAAAAATAGATCAAGATTTAAAACAAAATGGTATCAAAAGTACCTTAGCTAAAAAATATCTTGAAGAATTAAAAACAATAGGAGAAAAATATGGAATATAG", "aa": "MEKKKGKGKIMHEIKNNKFDEILLSEESKQKIDQDLKQNGIKSTLAKKYLEELKTIGEKYGI", "aa_hexdigest": "f1ce7457fea8a13f6a0f5878f8f91e20", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C02A5", "ncbi_nrp_id": "WP_013526823.1", "uniref100_id": "UniRef100_C4XE09", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C02A5", "RefSeq:WP_013526823.1", "UniRef:UniRef100_C4XE09" ] }, "ips": { "uniref100_id": "UniRef100_C4XE09", "uniref90_id": "UniRef90_C4XE09" }, "psc": { "uniref90_id": "UniRef90_C4XE09", "uniref50_id": "UniRef50_C4XE09" }, "pscc": { "uniref50_id": "UniRef50_C4XE09", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE09" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7209.3438000000015, "isoelectric_point": 9.331879234313966 }, "id": "OAIKOHHJFB_253", "locus": "GCA000209735_00221" }, { "type": "cds", "sequence": "contig_1", "start": 121093, "stop": 121593, "strand": "+", "frame": 1, "gene": null, "product": "HpyAIV family type II restriction enzyme", "db_xrefs": [ "EC:3.1.21.4", "GO:0009036", "GO:0009307", "SO:0001217", "UniRef:UniRef50_C4XE10", "UniRef:UniRef90_C4XE10" ], "nt": "ATGGAATATAGTCAATTTGAAGCAGAACTTAAAAGCGCCTTAACAAATGACCCTACATTAAAACTTTTAAAAGAAATAATCAATAAACCTTTTAATTTTGTTTCACCAACCAACCCATTTGATGTTAAGACTAAAATTAAACAATCATTTTTGAAGCATCAAGAAAGAATTTATGACAAATTTATTCAATATTGTGCTTTAAGTTTAATTACTCAATATGGTTTTAGTTTACAAAGAAGCAATTTTAGATACAATTTGCCTCTTAAAGAGGGACAACACTTAGGAGACATTCCTGAACAAGTAATAATCAATGCATCTTTCATTTTTAGATTGAATAATAATTCAACAAAAGATGAACCAACAGATATGTTTGTTGTTTATTTAAGAAAGAGAGATTGCTATTCACATAAAGCAATGAAAAAAACTTGTGAGAATATCATTAAACAATTAGAAGCCTTAAAAATTATTTACCCAAAAATGAATATTAAGGGTGCTTTATGA", "aa": "MEYSQFEAELKSALTNDPTLKLLKEIINKPFNFVSPTNPFDVKTKIKQSFLKHQERIYDKFIQYCALSLITQYGFSLQRSNFRYNLPLKEGQHLGDIPEQVIINASFIFRLNNNSTKDEPTDMFVVYLRKRDCYSHKAMKKTCENIIKQLEALKIIYPKMNIKGAL", "aa_hexdigest": "def91864d865c35b1e877f7777dd1f12", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 121093, "stop": 121896, "observations": { "mutation": [ 121591, 121714, 121840 ], "stop": [ 121591, 121714, 121840 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE10", "query_cov": 1.0, "subject_cov": 0.5763888888888888, "identity": 1.0, "score": 335.0, "evalue": 1.55e-114, "gene_start": 1, "gene_end": 166, "reference_start": 1, "reference_end": 166, "reference_sequence": "MEYSQFEAELKSALTNDPTLKLLKEIINKPFNFVSPTNPFDVKTKIKQSFLKHQERIYDKFIQYCALSLITQYGFSLQRSNFRYNLPLKEGQHLGDIPEQVIINASFIFRLNNNSTKDEPTDMFVVYLRKRDCYSHKAMKKTCENIIKQLEALKIIYPKMNIKGALWFLDNDYIKNIRLFNNFYYEQVDLRPYYGADFFEILDKKEDWLKIESFIKKFKENNQNYFSKMPDLDQSDEALIALLYLEKSEWTKLNSNKEIYRNIKENIFNLNDKNSNLFKAIAKRNKSY" }, "paralog": false, "identity": 0.9887640449438202, "score": 528.0, "evalue": 2.52e-193, "description": "Internal stop codon at 121591, 121714, 121840. Nonsense mutation around 121591, 121714, 121840.", "psc": { "uniref90_id": "UniRef90_C4XE10", "product": "HpyAIV family type II restriction enzyme", "ec_ids": [ "3.1.21.4" ], "uniref50_id": "UniRef50_C4XE10", "go_ids": [ "GO:0009036", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE10", "EC:3.1.21.4", "UniRef:UniRef50_C4XE10", "GO:0009036", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE10", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE10" ] } }, "id": "OAIKOHHJFB_254", "locus": "GCA000209735_00222" }, { "type": "gap", "sequence": "contig_1", "start": 122202, "stop": 122301, "strand": ".", "length": 100, "id": "OAIKOHHJFB_1796" }, { "type": "cds", "sequence": "contig_1", "start": 122312, "stop": 122587, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAATTGGAACGATAGTTCCTTTAGCAAGTTTAACGACGAGTTGTTTTGGTCCTATTTTTGAGCCACATCAACCTCCAAATCCAAATCCGAACCCTAATCCAAACCCTAGTGGCGGAGAAAGCACGGATTGGAGCGATGTGGATGGCTATGTACATTATGATGCAGAAAAGAATCCAAAAGATTTTCTAAAGAAGGACAATCAATATTATTTTGCACAAGTATTGCATAATGAGCAAAAACATGATGCCGATGTGTATAGAAATGATAATTGA", "aa": "MAIGTIVPLASLTTSCFGPIFEPHQPPNPNPNPNPNPSGGESTDWSDVDGYVHYDAEKNPKDFLKKDNQYYFAQVLHNEQKHDADVYRNDN", "aa_hexdigest": "ae43d046df7e4bf851f103dbb97d06d4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10230.988599999999, "isoelectric_point": 4.691001701354979 }, "id": "OAIKOHHJFB_255", "locus": "GCA000209735_00223" }, { "type": "cds", "sequence": "contig_1", "start": 122603, "stop": 122866, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAAAATCAATGTTCGTTTAGACTTGGATAAAGACTGGTATACTAGCCAACCTGAATTAAACGAAATTAAAGATTTATTCGATGTTGATTATAGCGCGCCGGTGAAGTTGAATAATATGATGAGAAACTTTTTGAATCCTGAATATAAAGATTTAGGTCTTGTGGATTCAAAGAATTATATTAATTCAATGGCTAGTTATTTAGAAGGACCGCAAAAAGATGTATTTGCCTATAAGGATAGTTATCATGGTAATAAATGA", "aa": "MPKINVRLDLDKDWYTSQPELNEIKDLFDVDYSAPVKLNNMMRNFLNPEYKDLGLVDSKNYINSMASYLEGPQKDVFAYKDSYHGNK", "aa_hexdigest": "14665cc564c7c5abfd5e5a988a3ba75f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10213.393699999997, "isoelectric_point": 4.955473518371581 }, "id": "OAIKOHHJFB_256", "locus": "GCA000209735_00224" }, { "type": "cds", "sequence": "contig_1", "start": 122867, "stop": 123193, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGAGGACCAAAAGGAAGTAAGAGAACACTATCACTATTATTTTGATATGGACCTTGATAATTACTATAAACAATTCATCAATGATTTTGCTCTTAAAGGCACAAGCAAGACATTTAAAGATATTGCTATTGATGGTTTATATAAACAAGGAATATGGAATAATTATTTCGACTTAGGAAGCGATTATTCAAAATGGTTTTATTTAGCTTTTACAAGAAGTAATGTTGAAAAATACCATGAAAGCTTTAAGATTTATGAGACTGCAAAACAAAACAAAGATTTCATAATGCAATTGCCTACGGGTGATGATTATAATCTTTGA", "aa": "MFEDQKEVREHYHYYFDMDLDNYYKQFINDFALKGTSKTFKDIAIDGLYKQGIWNNYFDLGSDYSKWFYLAFTRSNVEKYHESFKIYETAKQNKDFIMQLPTGDDYNL", "aa_hexdigest": "a0ba9ed63962212ed2112a9f49446b5d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13219.521199999988, "isoelectric_point": 5.049883079528808 }, "id": "OAIKOHHJFB_257", "locus": "GCA000209735_00225" }, { "type": "cds", "sequence": "contig_1", "start": 123218, "stop": 123583, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTGGTAAGATGGTTAAGCTAACGTTAGTCAATAACGAAGCTTATAAAAAGTATTTAGAGTTATTGCAAATGTTTGGAACTATTCAAGCTGGAAATAAGAAGTGGTTTGAAATTGAACCAATATATAGAAATTATTTTAAAGAGCACTTGGATAAAGCAACAAATGCATTGCTTAATGTTCTTAAATATGGAGCAGTCTTTGGTGTGGAAATGGAAGAATTAGGCAAAGAGAAAAAAACCTTAGTTAAGATGTTTCCAGTAGCTATTGGTGATAGTCAATTTGCTGATTATGAATATAGCTACGTGTGGGCGTATAGAACACTATATGATGTGCTAATGCCTATGTATGGGGCTAATGATTGA", "aa": "MAGKMVKLTLVNNEAYKKYLELLQMFGTIQAGNKKWFEIEPIYRNYFKEHLDKATNALLNVLKYGAVFGVEMEELGKEKKTLVKMFPVAIGDSQFADYEYSYVWAYRTLYDVLMPMYGAND", "aa_hexdigest": "bda667bc8220b44beca36b09b39af68d", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14106.255799999994, "isoelectric_point": 6.592834281921387 }, "id": "OAIKOHHJFB_258", "locus": "GCA000209735_00226" }, { "type": "cds", "sequence": "contig_1", "start": 123587, "stop": 123793, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCATATAGTTTTACTGAATATGATAATCAAAAGTCAGGCAATACACCATATGCTCCAATTTACGAATACATAAACTTTTTTACAAATACTGTTTTTAAAGCTAAAGATAAAAGTTTTAAAGAACTTGAATTAAACACTAAATTTATGAATAAAATGCAAGAGGCTGAATATAAAAAAGAAGCTAAAAGTAAGTTTCATAGCTGA", "aa": "MPYSFTEYDNQKSGNTPYAPIYEYINFFTNTVFKAKDKSFKELELNTKFMNKMQEAEYKKEAKSKFHS", "aa_hexdigest": "b716a7d05e6a6399036cfb1c65aa8a41", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8168.116399999998, "isoelectric_point": 8.803818702697757 }, "id": "OAIKOHHJFB_259", "locus": "GCA000209735_00227" }, { "type": "gap", "sequence": "contig_1", "start": 123997, "stop": 124096, "strand": ".", "length": 100, "id": "OAIKOHHJFB_1797" }, { "type": "cds", "sequence": "contig_1", "start": 124107, "stop": 124610, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTATACAGAAAGATTACGATTAAGAAAAAATAGATTCGTTAATAGCAGAAGAAATACAAAATTAACACGTTATGTTCATTTGTATGATGAAGAACACTTGTATATTAGTGAAAAGAATATAACTACTTTGAACTATATATCAGATACTACAAAGCTTGTGCGTAAGCTTGATTGCGGGACAATACATACCACGCAAAATCCTAGTGATTATGCTTCAAGCACGGCAGTTGAACAAATTGCTAAAAAGATTACGGGGAACTGTCAATATTCATTTTTTCTAGGGTTAGTAGGAGATGATATTGAGGCGGTTAAAAAGTTATACAAAACGGCTGAAGCTCCATTACTAGATTCTGAAGTTAAGTTTATTTCAGGAAGAAGAGTGGGGAAAGTTTTAGCAAGTATGAGTAATGCACTTAGATACAAAATTAATCTACATTACAATCCAAAGGAAAAGGAATTATTCTTTGAAAAGGGGGAAGATGAAAACATGAGAAATTAG", "aa": "MLYTERLRLRKNRFVNSRRNTKLTRYVHLYDEEHLYISEKNITTLNYISDTTKLVRKLDCGTIHTTQNPSDYASSTAVEQIAKKITGNCQYSFFLGLVGDDIEAVKKLYKTAEAPLLDSEVKFISGRRVGKVLASMSNALRYKINLHYNPKEKELFFEKGEDENMRN", "aa_hexdigest": "c35085f5a7ae73bcaf330acbf3abed42", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19437.9694, "isoelectric_point": 9.333942222595216 }, "id": "OAIKOHHJFB_260", "locus": "GCA000209735_00228" }, { "type": "gap", "sequence": "contig_1", "start": 125079, "stop": 125178, "strand": ".", "length": 100, "id": "OAIKOHHJFB_1798" }, { "type": "cds", "sequence": "contig_1", "start": 125675, "stop": 125872, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGAAAATTCATATATTTCATGTGGAAGTAAAAATGAAATTTATGATTATTTAAAAAAGAAAAATAAAGTTAAATTTATTCATCAAAATGATCAAATTCATTTCAAAACTTCAAGTAAAGAAATATATGAACTAACTGACACAATTAAGACTTTTGAAGAATTTAAATTGAATGAAAAAGATTATGATTTTTAA", "aa": "MTENSYISCGSKNEIYDYLKKKNKVKFIHQNDQIHFKTSSKEIYELTDTIKTFEEFKLNEKDYDF", "aa_hexdigest": "688b8295a0d7b972ecd5bec4c87494d4", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7909.776199999997, "isoelectric_point": 6.071563911437988 }, "id": "OAIKOHHJFB_261", "locus": "GCA000209735_00229" }, { "type": "cds", "sequence": "contig_1", "start": 126050, "stop": 126334, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGTTGTGTTTATTAATTATATTGACTTCAATTTCAATAGTTCTGATGTTGTCATTTTTTGAAAAGTCATATAAGATTGAAATTTTAAAATGGGCTACTGTTGCAATTTTGATTATAACAAATTTGTTTTGCGGAATAAGTTTTACATTTTCAAGAAAGGCAAAAGTTATTGAAAAAAGTCGAAATTTGGCAAAAATTAATCTTAATAATTCATTAAATTATTTATTTAAATTACTTAATTTTGAATACAAAAATGAAGAAAAAAATAACAATATTCCATGA", "aa": "MGLCLLIILTSISIVLMLSFFEKSYKIEILKWATVAILIITNLFCGISFTFSRKAKVIEKSRNLAKINLNNSLNYLFKLLNFEYKNEEKNNNIP", "aa_hexdigest": "7f2ddd0760bd29f8ea5d9ede8ebf7c38", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10878.915399999985, "isoelectric_point": 9.554681968688964 }, "id": "OAIKOHHJFB_262", "locus": "GCA000209735_00230" }, { "type": "cds", "sequence": "contig_1", "start": 126344, "stop": 126463, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAAACATTTAATCAATTTTTATCTCGTTCATATGTTCCTTTGTCTAAACATTTAAATGAACAATTAAATAAAGAAGATTCAGAAAACAAAAAGCTTAGTAAATCTTTCTTATGA", "aa": "MKETFNQFLSRSYVPLSKHLNEQLNKEDSENKKLSKSFL", "aa_hexdigest": "fcb684531ba649301cf0f7cda11a0bc9", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4659.2364, "isoelectric_point": 9.224345970153806 }, "id": "OAIKOHHJFB_263", "locus": "GCA000209735_00231" }, { "type": "cds", "sequence": "contig_1", "start": 126626, "stop": 126715, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGTTTCAGTTAATCACTTTGAACAATATTTTGATAAAAACAAAGATTTTGGATTATTTTTAAAAAGAGATTTAATTAGAGGCTGA", "aa": "MEVSVNHFEQYFDKNKDFGLFLKRDLIRG", "aa_hexdigest": "a0119ad52b43e4144f5ae7d993f6585b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3547.004200000001, "isoelectric_point": 6.52206974029541 }, "id": "OAIKOHHJFB_264", "locus": "GCA000209735_00232" }, { "type": "cds", "sequence": "contig_1", "start": 127097, "stop": 127342, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGAAAACAAAATTCGATTGTTACAAATATTGACAAAGGTCTAAATGAAGAAGATATTTACAAGCAAATTTTTAACCAAATTAACTATAAAATAGTTGAGTCGGAATATTTAGAAAATGTAAGTGAACAAGAAGAAAAGAATTTTTTATCAAGAGAATTAAAGAAAAAAAGTAATATAGAAGAAAGAAAAGAATTAACTAAAAAAATAAATGATATTTTAATTAATGAAATACCTTTTAACTGA", "aa": "MRKQNSIVTNIDKGLNEEDIYKQIFNQINYKIVESEYLENVSEQEEKNFLSRELKKKSNIEERKELTKKINDILINEIPFN", "aa_hexdigest": "a09586b7cf99781ca23b287d946dad0a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9762.92729999999, "isoelectric_point": 5.2397253036499025 }, "id": "OAIKOHHJFB_265", "locus": "GCA000209735_00233" }, { "type": "cds", "sequence": "contig_1", "start": 127466, "stop": 127636, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAGATTTTTGTACCAAAGACTTTTGCGGAATTTAAAGATACAAATAAACAGTTTGTAAATGACAAAATACAATTAATTATAGACAATAAAAATAAAGAAATTAAGACAAGTTTTTTATATTTAATTACATCAATTGTTTTAATATTTCTAGGAATAATTTTTTGA", "aa": "MAKIFVPKTFAEFKDTNKQFVNDKIQLIIDNKNKEIKTSFLYLITSIVLIFLGIIF", "aa_hexdigest": "918a411f3120123dc14306e17b3dd998", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6553.787999999997, "isoelectric_point": 9.45688343048096 }, "id": "OAIKOHHJFB_266", "locus": "GCA000209735_00234" }, { "type": "cds", "sequence": "contig_1", "start": 128332, "stop": 128808, "strand": "-", "frame": 2, "gene": null, "product": "DUF3137 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE16", "UniRef:UniRef90_C4XE16" ], "nt": "ATGGGAGTTACAACTAAACCAAAGAGTTATTTAGATTTTGTGAATGAAAACTTACAATCATTTAAAGAAATAGTTTCAAACAGTTTTGATTCAGTTATTAAAAATAAAAAGAATATTTTGTCAAAATTAAAAATATTACTAATTGTTTCAATAGTACTATTAGTTTTAGGTGGAATTGGATTAATATTATCTTTCTTTTTCTTCATAAAAACTAATTCAAAAAATACAGCAATATTTGCTGGATTAATTATTTCTGCAATAGTAACAGGAATTGGTTCAATTGTATTATCAGTATTTTTAGGAAAAAGGAAAAAACTTTTTAAAGAACTCAAACACGTTCTTGATAAAGAAAAATTATTTGACAAAGCTTTTGAAAAATTATCATACAAAGCAATCGATCCAAATGACCCAGAACAAGCACAATTATTAGAAAAATTTAAACAAGGCTATGATAAAAATAGAGATTGTTTAGACTGA", "aa": "MGVTTKPKSYLDFVNENLQSFKEIVSNSFDSVIKNKKNILSKLKILLIVSIVLLVLGGIGLILSFFFFIKTNSKNTAIFAGLIISAIVTGIGSIVLSVFLGKRKKLFKELKHVLDKEKLFDKAFEKLSYKAIDPNDPEQAQLLEKFKQGYDKNRDCLD", "aa_hexdigest": "08c5df4cc96faed715c139dffb82d168", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 128029, "stop": 128808, "observations": { "mutation": [ 128208, 128235, 128307, 128334 ], "stop": [ 128208, 128235, 128307, 128334 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE16", "query_cov": 1.0, "subject_cov": 0.4328767123287671, "identity": 1.0, "score": 290.0, "evalue": 8.36e-96, "gene_start": 1, "gene_end": 158, "reference_start": 1, "reference_end": 158, "reference_sequence": "MGVTTKPKSYLDFVNENLQSFKEIVSNSFDSVIKNKKNILSKLKILLIVSIVLLVLGGIGLILSFFFFIKTNSKNTAIFAGLIISAIVTGIGSIVLSVFLGKRKKLFKELKHVLDKEKLFDKAFEKLSYKAIDPNDPEQAQLLEKFKQGYDKNRDCLDWVDHIPGAYWIPSDANVDSTGKIYFVMDKNENPWVIQTVRYHWVREERDLKGNITIRHIYDYATLFEGIFYSLPKSKNFTLAIGNRCGLTNNLKQNIKYENQEFDKLFKAYCSDSLKGHNIYQPYPMEITLEHCKQNKSYVKSFGMLLDDQNIKCWVRPRGSILEIDMPSNTINKDKIVKTVCKDILEDTYSVYWLFSLLTIPGYFN" }, "paralog": false, "identity": 0.9845559845559846, "score": 498.0, "evalue": 4.54e-180, "description": "Internal stop codon at 128208, 128235, 128307, 128334. Nonsense mutation around 128208, 128235, 128307, 128334.", "psc": { "uniref90_id": "UniRef90_C4XE16", "product": "DUF3137 domain-containing protein", "uniref50_id": "UniRef50_C4XE16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE16", "UniRef:UniRef50_C4XE16" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE16" ], "product": "DUF3137 domain-containing protein" } }, "id": "OAIKOHHJFB_267", "locus": "GCA000209735_00235" }, { "type": "cds", "sequence": "contig_1", "start": 129046, "stop": 129180, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59BDB", "UniRef:UniRef100_A0AB32XAH3", "UniRef:UniRef50_A0AB32XAH3", "UniRef:UniRef90_A0AB32XAH3" ], "nt": "ATGGAATTAGAATTTCTAGAATTTAATTATTTATTTAAGTCCCCTATTCAAATAGTAAATTATTATTCTATGAAAAAAATGATTAAAGTTGCAAAACTAAATTCAGAATTATTAAAACTTTTGGTATGCAATTAG", "aa": "MELEFLEFNYLFKSPIQIVNYYSMKKMIKVAKLNSELLKLLVCN", "aa_hexdigest": "2cd743ec5c70526871689779ff580ecb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59BDB", "uniref100_id": "UniRef100_A0AB32XAH3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59BDB", "UniRef:UniRef100_A0AB32XAH3" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XAH3", "uniref90_id": "UniRef90_A0AB32XAH3" }, "psc": { "uniref90_id": "UniRef90_A0AB32XAH3", "uniref50_id": "UniRef50_A0AB32XAH3" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XAH3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XAH3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5287.3682, "isoelectric_point": 8.85242786407471 }, "id": "OAIKOHHJFB_268", "locus": "GCA000209735_00236" }, { "type": "cds", "sequence": "contig_1", "start": 129238, "stop": 130044, "strand": "-", "frame": 2, "gene": "rlmD", "product": "23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD", "db_xrefs": [ "EC:2.1.1.190", "GO:0008649", "KEGG:K03215", "SO:0001217", "UniRef:UniRef50_C4XE18", "UniRef:UniRef90_C4XE18" ], "nt": "ATGTATTTAAACAAAAATATTAACAATTACAAATGTTTTATAGAAAACGGACAATTTTTAAAACAAATTGTTGTTAGATATTCAGAATCATATGATCAAATGATGTTAGCTTTTGTTCAAGACAAACCATTTGAATTATCTGAAGATTTATTAAAAGATTTAACCTCAAATTTTAAAAATTTAAAAGTTATATTAAAAGAAGAAAAAGTTAAAAAAACAACTTATACTTATTATTCAGAAGCTAAAAACATACAAGACAAAATTAGCAATTTAACTTTTAATATTGATTACAATAGTTTCTTTCAAGTTAACTCGTATCAAACCAATAATTTATATAACTTGCTAGTTAATAATTTGCACCTTGATAAAACTATGAATGTAGTTGATGCATATTCTGGAATCGGAACAATAAGTTTAAAAATTGCAGAAAAAGTTAAAAAAGTTTATGGACTTGAAATAGTAAAAGAAGCAGTTATAAATGCTAAGCAAAATGCAAAATTAAATAACATAAATAATACAGAATTTTATGAAGGCGATGTTTTAAAAACTATTGATAACATTAAAGAAAAAATAGACACAATAATAATAGATCCTCCAAGAGCAGGAGTATCGAAAGAAGTTTTAGAAAAAATATTAAAAATTGAACCAAAACAAATTGGTTACATAAGTTGTAATCCTCACACACTTTGCAGAGATATAGATTTATTAACATTATCAAAAAAATACAAATTAGAATTTTTAAAACCATGTGATATGTTTTGTCAAACACACCATGTAGAAACAGTTGCAATTTTAAAATTAAAATAA", "aa": "MYLNKNINNYKCFIENGQFLKQIVVRYSESYDQMMLAFVQDKPFELSEDLLKDLTSNFKNLKVILKEEKVKKTTYTYYSEAKNIQDKISNLTFNIDYNSFFQVNSYQTNNLYNLLVNNLHLDKTMNVVDAYSGIGTISLKIAEKVKKVYGLEIVKEAVINAKQNAKLNNINNTEFYEGDVLKTIDNIKEKIDTIIIDPPRAGVSKEVLEKILKIEPKQIGYISCNPHTLCRDIDLLTLSKKYKLEFLKPCDMFCQTHHVETVAILKLK", "aa_hexdigest": "53bd3085092ef572d0638fc831bfa033", "start_type": "ATG", "rbs_motif": null, "genes": [ "rlmD" ], "truncated": "3-prime", "pseudogene": { "start": 129238, "stop": 130344, "observations": { "mutation": [ 130185 ], "stop": [ 130185 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE18", "query_cov": 1.0, "subject_cov": 0.6077097505668935, "identity": 1.0, "score": 525.0, "evalue": 1.37e-185, "gene_start": 1, "gene_end": 268, "reference_start": 174, "reference_end": 441, "reference_sequence": "MRNMKYELGQIIKGVEAINFTYEALGLVKADDYSIFVENLLPQEKADIQIKKSNTKFAFAIVVKRYNDSPKRIKVLNQKLMLSGSTPLAILNYADQLEFKENVVKYLFQRNIHFNETQKILPCEKQWNYRNKITVFVESSKGKIKFGLYEKNTHKLVEQDSYDLANESINKLLMYLNKNINNYKCFIENGQFLKQIVVRYSESYDQMMLAFVQDKPFELSEDLLKDLTSNFKNLKVILKEEKVKKTTYTYYSEAKNIQDKISNLTFNIDYNSFFQVNSYQTNNLYNLLVNNLHLDKTMNVVDAYSGIGTISLKIAEKVKKVYGLEIVKEAVINAKQNAKLNNINNTEFYEGDVLKTIDNIKEKIDTIIIDPPRAGVSKEVLEKILKIEPKQIGYISCNPHTLCRDIDLLTLSKKYKLEFLKPCDMFCQTHHVETVAILKLK" }, "paralog": false, "identity": 0.9972826086956522, "score": 729.0, "evalue": 2.62e-268, "description": "Internal stop codon at 130185. Nonsense mutation around 130185.", "psc": { "uniref90_id": "UniRef90_C4XE18", "gene": "rlmD", "product": "23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD", "ec_ids": [ "2.1.1.190" ], "uniref50_id": "UniRef50_C4XE18", "kegg_orthology_id": "K03215", "go_ids": [ "GO:0008649" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE18", "EC:2.1.1.190", "UniRef:UniRef50_C4XE18", "KEGG:K03215", "GO:0008649" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE18", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE18" ], "product": "TRAM domain-containing protein" } }, "id": "OAIKOHHJFB_269", "locus": "GCA000209735_00237" }, { "type": "cds", "sequence": "contig_1", "start": 130183, "stop": 130554, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01938.26" ], "nt": "ATGAAATATGAATTAGGCCAAATAATAAAAGGAGTGGAAGCAATAAATTTTACTTATGAAGCATTAGGCTTAGTTAAAGCAGATGATTATTCTATTTTTGTTGAGAATTTATTGCCACAAGAAAAAGCAGATATACAAATTAAAAAATCGAACACAAAATTTGCTTTTGCTATAGTTGTAAAAAGATATAACGATAGTCCAAAAAGAATAAAAGTTTTAAATCAAAAATTAATGCTAAGTGGTTCAACTCCATTAGCAATTTTAAACTATGCAGATCAATTAGAATTTAAAGAAAATGTTGTTAAATATTTATTTCAAAGAAATATCCATTTTAATGAAACACAAAAAATTTTGCCGTGTGAAAAACAATGA", "aa": "MKYELGQIIKGVEAINFTYEALGLVKADDYSIFVENLLPQEKADIQIKKSNTKFAFAIVVKRYNDSPKRIKVLNQKLMLSGSTPLAILNYADQLEFKENVVKYLFQRNIHFNETQKILPCEKQ", "aa_hexdigest": "b300418954365ea01cdf738fe394eb96", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01938.26", "name": "TRAM", "length": 40, "aa_cov": 0.3089430894308943, "hmm_cov": 0.975, "evalue": 4.3316837124135513e-07, "score": 25.837770462036133, "start": 23, "stop": 60 } ], "seq_stats": { "molecular_weight": 14244.428599999983, "isoelectric_point": 9.118231010437015 }, "id": "OAIKOHHJFB_270", "locus": "GCA000209735_00238" }, { "type": "cds", "sequence": "contig_1", "start": 130628, "stop": 131221, "strand": "-", "frame": 1, "gene": null, "product": "M24 family metallopeptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00313C6812" ], "nt": "ATGACAGAAAAAGAAGTAGCTGCATACTTAAATTATTTAATGAAAAAACATGGATCAGATAAAGAAAGTTTTGATGAAATAGTTGCTACAGGGCCTTCATCAGCTGAACCTCACCACCATCCAACAGATAGAAAATTAAAAGAAGGGGATCTTCTTAAAATCGACTTTGGTGCTCTTTACAAAGGATACAGTGCAGATATTACTAGAACATGTATTTTAGGTGGAGACAAAAAAGCGAATGATCCTAAACAATTAGAAATTTTACAAATAGTTATGGAAGCTGCAAAAGCTGGTCGTGATGCAGTAAGACCTGGAATTAAAGCAAGTGAAATTGATAAAATTTGTCGTGATTACATAACTAAAAAAGGATATGGTAAATACTTTGTTCACTCAACTGGTCATGGACTTGGAATTGATGTACATGAATTACCTAATGTTAGATCAACAAGTGACTATATTTTAGAACCTGGAATGATAATTACTGTTGAACCTGGTATTTATATTGAAGGTTTAGGCGGAGCAAGAAATGAAGATGATGTTCTTGTTACAGAAAAAGGTCGTTATGTATTCAGTAGACCTGAAGAAAAATCTTAG", "aa": "MTEKEVAAYLNYLMKKHGSDKESFDEIVATGPSSAEPHHHPTDRKLKEGDLLKIDFGALYKGYSADITRTCILGGDKKANDPKQLEILQIVMEAAKAGRDAVRPGIKASEIDKICRDYITKKGYGKYFVHSTGHGLGIDVHELPNVRSTSDYILEPGMIITVEPGIYIEGLGGARNEDDVLVTEKGRYVFSRPEEKS", "aa_hexdigest": "bbf05903b3e9a818f78b4c01a085a7bf", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00313C6812", "query_cov": 0.8883248730964467, "subject_cov": 0.8663366336633663, "identity": 0.514, "score": 177.0, "evalue": 4.17e-53, "valid": false, "product": "M24 family metallopeptidase", "uniref50_id": "UniRef50_UPI00313C6812" }, "pscc": { "uniref50_id": "UniRef50_UPI00313C6812", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00313C6812" ], "product": "M24 family metallopeptidase" }, "genes": [], "id": "OAIKOHHJFB_271", "locus": "GCA000209735_00239" }, { "type": "cds", "sequence": "contig_1", "start": 131752, "stop": 132579, "strand": "-", "frame": 2, "gene": "era", "product": "GTPase Era", "db_xrefs": [ "GO:0003723", "GO:0005525", "SO:0001217", "UniRef:UniRef50_Q98QI1", "UniRef:UniRef90_C4XE20" ], "nt": "ATGAAAGTATGTTTAGCTAGTATTATCGGCAGACCTAATGTAGGAAAATCATCATTAATAAATCAAATTGTTAAATATGATGTTGCAATTGTAACTAATGTTCCGCAAACAACAAGAGATCAAATTGTCGGCGTTTATAACGAAGATGATTTTCAATTAGTTTTTGTTGATACACCAGGTATCCACAAACCATTAAATTTATTAGGTGAAGCCTTAAATAAAAGTGCTTTTGAATCTACAAAAGATGTTGATTGTTTAATATTTCTTTCTCCTGTTAATGAAAAAGTTGGACAAGGAGATATTTTAATTTTAGATAAGATCAAAAATACCAAGAATAAAATTGCAGTCATTTCTAAAATTGATTTAGCCAAAAAACCAGAAGAAATACAAGAAAAAATTGAAGAATTAAGCAAATACAGTTTTGACAAAATATTGTCTGTTTCAAGAAATAATGAAAACTCAATTGAAGAATTAATTAAAGTTATTAAGCAATATGCCTATGAAGGTGAGCCATTTTATGATGAAGACTATATAACAGATAAATCAATGAGATTTTTAGCAAAAGAGATAATAAGAGAAAGTGCGATTAATCTTTTAAGAGAAGAGTTGCCACACTCAATTGCTGTAGAAGTTATTGATTTTATTGAAGAAGATGATATTAAAATAAATGCCATCATTTATGTCAAAAAAGCTTCTCAAAAAGGAATGGTAATTGGCAAAAATGGTCAAATGATAAAACAAATAGGAACCAACGCAAGAAAGAAAATGATGAGTCAATTTGATTCAAAAGTGGAATTAAATTTAAAAGTTAAAATAGCAAACAAATGA", "aa": "MKVCLASIIGRPNVGKSSLINQIVKYDVAIVTNVPQTTRDQIVGVYNEDDFQLVFVDTPGIHKPLNLLGEALNKSAFESTKDVDCLIFLSPVNEKVGQGDILILDKIKNTKNKIAVISKIDLAKKPEEIQEKIEELSKYSFDKILSVSRNNENSIEELIKVIKQYAYEGEPFYDEDYITDKSMRFLAKEIIRESAINLLREELPHSIAVEVIDFIEEDDIKINAIIYVKKASQKGMVIGKNGQMIKQIGTNARKKMMSQFDSKVELNLKVKIANK", "aa_hexdigest": "fd3af8ade559fc7fb09080e965318b26", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_C4XE20", "query_cov": 1.0, "subject_cov": 0.9482758620689655, "identity": 1.0, "score": 518.0, "evalue": 3.06e-185, "valid": true, "gene": "era", "product": "GTPase Era", "uniref50_id": "UniRef50_Q98QI1", "go_ids": [ "GO:0003723", "GO:0005525" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QI1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QI1" ], "product": "GTPase Era" }, "genes": [ "era" ], "id": "OAIKOHHJFB_272", "locus": "GCA000209735_00240" }, { "type": "cds", "sequence": "contig_1", "start": 132579, "stop": 132701, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAGAATTTATGCCCGATGATGGTTTAATTTACCAATACAATAAAAATGGCGACAAATGTAGAGTAAATAAAGTAATTGAAATGGTCCCATTTCCTATTAGAACAGAGCAAATTAAATAA", "aa": "MTEFMPDDGLIYQYNKNGDKCRVNKVIEMVPFPIRTEQIK", "aa_hexdigest": "2888be777c1f96d3b037da4c5c27f0e2", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4762.5082, "isoelectric_point": 6.058149909973144 }, "id": "OAIKOHHJFB_273", "locus": "GCA000209735_00241" }, { "type": "cds", "sequence": "contig_1", "start": 132975, "stop": 133196, "strand": "-", "frame": 3, "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C77EC7" ], "nt": "ATGCCAATTTATCCCTTAGGTGAATTAGTAATAAGCCATGAAAAAGTTGAAGCACAAGCTCAAGAGTTTGTTCATTCAATTCGTAGAGAATATTGTTATTTATTTGCTCATGGATTAATTCACTTGATGGGATATGATCATGAAACAGAAGTCGAAAGAGCTGAAATGAATAAACTTGTTGATTTAATATTTGAACCATTAAAAATAAGTAGAGAGGAATAA", "aa": "MPIYPLGELVISHEKVEAQAQEFVHSIRREYCYLFAHGLIHLMGYDHETEVERAEMNKLVDLIFEPLKISREE", "aa_hexdigest": "798387293e6febc0af70aaa44c56a0e4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0024C77EC7", "query_cov": 0.8904109589041096, "subject_cov": 1.0, "identity": 0.631, "score": 90.1, "evalue": 1.98e-22, "valid": false, "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "uniref50_id": "UniRef50_UPI0024C77EC7", "go_ids": [ "GO:0004540", "GO:0042254", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0024C77EC7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C77EC7" ], "product": "rRNA maturation RNase YbeY" }, "genes": [ "ybeY" ], "id": "OAIKOHHJFB_274", "locus": "GCA000209735_00242" }, { "type": "cds", "sequence": "contig_1", "start": 133212, "stop": 133430, "strand": "-", "frame": 3, "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "db_xrefs": [ "GO:0004540", "GO:0042254", "GO:0046872", "SO:0001217", "UniRef:UniRef50_L0RUR5", "UniRef:UniRef90_C4XE22" ], "nt": "ATGGTTGAATTAAATGTTGTAAACAAGACTAAAAATAAATTTTTGTTTGAAAAAGAATACAAGCAAATATTAGAAAATTTAGCTAAAGAATTTGGGCTTAATCAAACTGTTTCAGTTGATGTAACTATTACAAATAATGATGAAATCCAAAAACTGAATAAAGAATATAGAAACAAAGATTATCCAACAGATATCTTGTCTTTTGGTTTAGAAGATTGA", "aa": "MVELNVVNKTKNKFLFEKEYKQILENLAKEFGLNQTVSVDVTITNNDEIQKLNKEYRNKDYPTDILSFGLED", "aa_hexdigest": "9720586fa9bf70882e50697d09f9bd76", "start_type": "ATG", "rbs_motif": null, "genes": [ "ybeY" ], "truncated": "5-prime", "pseudogene": { "start": 132975, "stop": 133496, "observations": { "mutation": [ 133214 ], "stop": [ 133214 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE22", "query_cov": 1.0, "subject_cov": 0.4161849710982659, "identity": 1.0, "score": 141.0, "evalue": 2.43e-41, "gene_start": 1, "gene_end": 72, "reference_start": 23, "reference_end": 94, "reference_sequence": "MCGSSHSIKFNIKFIINNIIRIMVELNVVNKTKNKFLFEKEYKQILENLAKEFGLNQTVSVDVTITNNDEIQKLNKEYRNKDYPTDILSFGLEDWELFENMPIYPLGELVISHEKVEAQAQEFVHSIRREYCYLFAHGLIHLMGYDHETEVERAEMNKLVDLIFEPLKISREE" }, "paralog": false, "identity": 0.9884393063583815, "score": 343.0, "evalue": 2.62e-123, "description": "Internal stop codon at 133214. Nonsense mutation around 133214.", "psc": { "uniref90_id": "UniRef90_C4XE22", "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "uniref50_id": "UniRef50_L0RUR5", "go_ids": [ "GO:0004540", "GO:0042254", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE22", "UniRef:UniRef50_L0RUR5", "GO:0004540", "GO:0042254", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_L0RUR5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RUR5" ], "product": "Endoribonuclease YbeY" } }, "id": "OAIKOHHJFB_275", "locus": "GCA000209735_00243" }, { "type": "cds", "sequence": "contig_1", "start": 133451, "stop": 133789, "strand": "-", "frame": 1, "gene": null, "product": "MAG1140 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LI05", "UniRef:UniRef90_A0AB32XAW0" ], "nt": "TTGTTAGCTATTGCAACAGTAACTTTAATTGTTTTTATTTGTTTATATCATGTTGACAAAACAATAAGAGTTAAGTTACAAGTTAATGACAAAAAAGACTTAACTTTGTTAGCAAATGATAAGACTGCTTATTTATTAAAGAAAAATCAAAAAATAATAATTAATGTAAATGACCAAATTTTCAATTTAAAAATCCGCAACTTAAAAATTGAAAATCAAACGATTAAAGTAACCTTCTATAATTTGCCTAATAGTTTGAAACTTTTACCTAATACAACTTTAGATGCCACGATATTTGTGGATCGAGCCACTCTATTAAATTTAATATTAAATTCATAA", "aa": "MLAIATVTLIVFICLYHVDKTIRVKLQVNDKKDLTLLANDKTAYLLKKNQKIIINVNDQIFNLKIRNLKIENQTIKVTFYNLPNSLKLLPNTTLDATIFVDRATLLNLILNS", "aa_hexdigest": "4928e7b648ebfbc2b2b36daba65916e0", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB32XAW0", "query_cov": 1.0, "subject_cov": 0.875, "identity": 0.991, "score": 202.0, "evalue": 1.92e-65, "valid": true, "product": "MAG1140 family protein", "uniref50_id": "UniRef50_A0A3P8LI05" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LI05", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LI05" ] }, "genes": [], "id": "OAIKOHHJFB_276", "locus": "GCA000209735_00244" }, { "type": "cds", "sequence": "contig_1", "start": 133812, "stop": 134618, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGATGTTCTTAAATGGAATAAATTTCTTTGTTAATCCAATGCTAAATATGAGCTCATTATTACTTTCTCAAACAATGATCAAAAGAAATGTTAATTTAGTAAATTTTGTTTTAAATTTAGACGAAAAAAGCAATTTAGATACTGGTTTATTCATCGATAAAATTAAAAAAATTGAATTAGAAAATGTTAATTTTTATTACGAAAACGGAAAAAATATTTTAAATATTCCTTCATTTACGATTGACCAAAATATTCAATTAAATGGTAAAAATGGTAGTGGTAAAACCACTATGCTAAGTTTTATAAACGGCTGTTTTAAAGGTTATTTAGGAAAAATGAAGATTAATGATATTGATTTTGATTCAATAAATTTGATTAATTATCAAAGTAAAAATATATTGATAAATTCTTCCAGCCATTTACCAGATGTTTCTTTTCTCGAATATATTACAAGTAACAATGAAAATTATAAAAATGAATTGAATAATAATATTCAAAAATATGATCTATACAAAATATTTGAAGAAATGAATTTTAATTTAAATGGCAGCATAATTAGTAATGGAAGCAATTTGTCAAGTGGACAAAAACAACTTTTATTATTACTAAAATTATTTGTAAGAAAATATGACTTAATCATGCTTGATGAAGCCTTTGAAAATATTAATCCAAGTAATTGTGAATTATTGAAAAAGGCTATAAAGAAATATCAAGATGAAGCATTATTTATTGAAATAAGTCACAATAGGAACTATTTATTTAAAACAAAGGAGGTGTGCTTTGAAAAAATTAACAACTGTTTCTAA", "aa": "MMFLNGINFFVNPMLNMSSLLLSQTMIKRNVNLVNFVLNLDEKSNLDTGLFIDKIKKIELENVNFYYENGKNILNIPSFTIDQNIQLNGKNGSGKTTMLSFINGCFKGYLGKMKINDIDFDSINLINYQSKNILINSSSHLPDVSFLEYITSNNENYKNELNNNIQKYDLYKIFEEMNFNLNGSIISNGSNLSSGQKQLLLLLKLFVRKYDLIMLDEAFENINPSNCELLKKAIKKYQDEALFIEISHNRNYLFKTKEVCFEKINNCF", "aa_hexdigest": "61d6e27bcc3f2d7163edc3a59f445cf4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 140, "aa_cov": 0.5149253731343284, "hmm_cov": 0.9, "evalue": 7.035788481651579e-18, "score": 61.646934509277344, "start": 82, "stop": 219 } ], "seq_stats": { "molecular_weight": 31045.39700000003, "isoelectric_point": 6.744537544250489 }, "id": "OAIKOHHJFB_277", "locus": "GCA000209735_00245" }, { "type": "cds", "sequence": "contig_1", "start": 134709, "stop": 134822, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAATGTAGATTATTCAAGATTTATTTCTAAAAACCAATTTAAAAACATGTTGGAATTTAAGAAAAAAGAATATTCATTTGCTTCTAAAGATGATATTAAAAACTTTTGA", "aa": "MDNVDYSRFISKNQFKNMLEFKKKEYSFASKDDIKNF", "aa_hexdigest": "5e6c384aeaedc959b54a9a48799bcbae", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4557.1226000000015, "isoelectric_point": 9.100179862976074 }, "id": "OAIKOHHJFB_278", "locus": "GCA000209735_00246" }, { "type": "cds", "sequence": "contig_1", "start": 134985, "stop": 135236, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATTAATCGGTTGTTTAAAAATCATGTTATTAAAAAAATAAAAAACGATTTAGAGTTTACTTTAACAAAAGCATTTTTTGACAAAATAAAAAGAGTGCCATCAAATTATTTATCTAAATTAACAAGCAATGATTACTTTAAAAGACTTACTTATATAAAAGAAATATGTGAATTTCAATCTAATTTTGTCTTTACTTTTTTAAGTGAAATATTTTCTTTTATAGGTTCAACAATCTTATTGATTTGA", "aa": "MFINRLFKNHVIKKIKNDLEFTLTKAFFDKIKRVPSNYLSKLTSNDYFKRLTYIKEICEFQSNFVFTFLSEIFSFIGSTILLI", "aa_hexdigest": "ada8443b97f9896a4645d090619f7959", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9958.638099999993, "isoelectric_point": 9.673174858093262 }, "id": "OAIKOHHJFB_279", "locus": "GCA000209735_00247" }, { "type": "cds", "sequence": "contig_1", "start": 135249, "stop": 135875, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAATAAAAAAACAAGAAGATATAAAAGACTGTGGTTTATATGTTTTGCAGTATTTTGTGGCAAAACATAATGATGACTACATTGATATTAATTATTTTAAAAATAAGGCAACTTACTCTTCAGAAGGAGTAAATATTTCAACACTTAAAAAATTAGCTTCTGAACACGATATCGATTTAAATAGTTATACAGGTGATTTTGAATCTCTTGAATCTTTGGACAATTCAAATATGCCTATAGCTTTATTATTAAATAGAAAAGGATATAGCCATTATGTAATTTTAGAAAAAATGAAGGACAATTATTTTATTGTTCAAGATCCAGCTTATGGTCGCAAGATGAAAGTTAAAAAGAAAGACTTGGCTAAGGAATTTGCCAATGTGATACTATTTTTTGATCCAAAAGAAGAAAAATCAAAAAATAAAAATAAAAACTATTCACTTGATAATAAAATAAGCACATTATTTTCATTTAAAAAATATACTTATCCTTTATTCATTTCAGCACTTTTAGGATTGTTTTTATCATTTGGATCAACATTTTTTATAAAAATAGTTTTTGATTTTATATTGCCTAATTATCTTAAAAATAATTTGATTATTATTTTTGTATTTTTCCTATGA", "aa": "MRIKKQEDIKDCGLYVLQYFVAKHNDDYIDINYFKNKATYSSEGVNISTLKKLASEHDIDLNSYTGDFESLESLDNSNMPIALLLNRKGYSHYVILEKMKDNYFIVQDPAYGRKMKVKKKDLAKEFANVILFFDPKEEKSKNKNKNYSLDNKISTLFSFKKYTYPLFISALLGLFLSFGSTFFIKIVFDFILPNYLKNNLIIIFVFFL", "aa_hexdigest": "54bc59a349a4f6b16e512665ed051fdc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24377.981800000027, "isoelectric_point": 9.086319160461429 }, "id": "OAIKOHHJFB_280", "locus": "GCA000209735_00248" }, { "type": "cds", "sequence": "contig_1", "start": 136008, "stop": 136454, "strand": "+", "frame": 3, "gene": null, "product": "Ribose-5-phosphate isomerase", "db_xrefs": [ "RefSeq:WP_013526638.1", "SO:0001217", "UniParc:UPI0001F59BD7", "UniRef:UniRef100_C4XE24", "UniRef:UniRef50_A0A449BAM0", "UniRef:UniRef90_C4XE24" ], "nt": "ATGGCTAAAAAAAATATTATTGCTCTTGCTAGCGACCATGGTGGTTGCGAATTAAAAAATGAAATGCGCGATTATGTAAAAAGTTTAGGTTATGAAGTTGTTGACTTAGGACCTGCGGATGGCTCAAAAAGTATTTCATATGCTGAACAAGGGCATAACCTAGCTAAATATATTGATGAACATAAAAACACAATTGGTTTAGGTTTTTGTGGTACAGGTTTAGGTATTTCATATGCCTTAAACCGTCATAAACACATTCGCGCAGCTAGAGTAGTTACAGTTGAAGATGCAAGGCTTGCTAAATTACATAATAATGCTAATGTTTTAGTTATGGGTGGTAGATATGTACCTTTTGCTGAAGCACAAGCTATGTTTAACGAATACATTAAAACTGAATATGAAGGTGGCAGACACCAAGCAAGAATCGATCAAATCGATGAATTCTAA", "aa": "MAKKNIIALASDHGGCELKNEMRDYVKSLGYEVVDLGPADGSKSISYAEQGHNLAKYIDEHKNTIGLGFCGTGLGISYALNRHKHIRAARVVTVEDARLAKLHNNANVLVMGGRYVPFAEAQAMFNEYIKTEYEGGRHQARIDQIDEF", "aa_hexdigest": "4f5c7f5bd1fa86bfcb99770a47fce7af", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001F59BD7", "ncbi_nrp_id": "WP_013526638.1", "uniref100_id": "UniRef100_C4XE24", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59BD7", "RefSeq:WP_013526638.1", "UniRef:UniRef100_C4XE24" ] }, "ips": { "uniref100_id": "UniRef100_C4XE24", "uniref90_id": "UniRef90_C4XE24" }, "psc": { "uniref90_id": "UniRef90_C4XE24", "product": "Ribose-5-phosphate isomerase", "uniref50_id": "UniRef50_A0A449BAM0" }, "pscc": { "uniref50_id": "UniRef50_A0A449BAM0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449BAM0" ], "product": "Ribose-5-phosphate isomerase B" }, "genes": [], "id": "OAIKOHHJFB_281", "locus": "GCA000209735_00249" }, { "type": "cds", "sequence": "contig_1", "start": 136521, "stop": 136895, "strand": "+", "frame": 3, "gene": null, "product": "Impact N-terminal domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354345.1", "SO:0001217", "UniParc:UPI0001E32F4A", "UniRef:UniRef100_C4XE25", "UniRef:UniRef50_D5E655", "UniRef:UniRef90_C4XE25" ], "nt": "ATGGAAAAAGCACTTTTATATGAAATCAAAAAATCAAAGTTTTATTCTTTAGCTTTTAATGTGACTAGTAAGGAACAAATAAAAGAAGTAGAAGCTAATTTAAGAAAAGAATACAAGAAAGCAACTCATATTTGTCATGGTTATAGCTTTTTAAATAATGGAGTGATCAATGCAGGCTTTAGCGATGATGGCGAACCTAATGGTACAGCCGGCAGACCAATTAGAGATCTTTTAATTATGCGCAAAATTAATAATGTTGTTGTTTTTGTAATTCGCTATTTTGGTGGAATTAAGTTGGGAGCCAACGGTTTAGCTAGAGCTTATATTAAAAGTGCAAACTTAGCTATAGAAGAATATATAAAGGAGGGAGCCTAA", "aa": "MEKALLYEIKKSKFYSLAFNVTSKEQIKEVEANLRKEYKKATHICHGYSFLNNGVINAGFSDDGEPNGTAGRPIRDLLIMRKINNVVVFVIRYFGGIKLGANGLARAYIKSANLAIEEYIKEGA", "aa_hexdigest": "28f4398ba41d5b95b867a2e497c60f17", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32F4A", "ncbi_nrp_id": "WP_013354345.1", "uniref100_id": "UniRef100_C4XE25", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F4A", "RefSeq:WP_013354345.1", "UniRef:UniRef100_C4XE25" ] }, "ips": { "uniref100_id": "UniRef100_C4XE25", "uniref90_id": "UniRef90_C4XE25" }, "psc": { "uniref90_id": "UniRef90_C4XE25", "product": "Impact N-terminal domain-containing protein", "uniref50_id": "UniRef50_D5E655" }, "pscc": { "uniref50_id": "UniRef50_D5E655", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E655" ], "product": "Impact N-terminal domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_282", "locus": "GCA000209735_00250" }, { "type": "cds", "sequence": "contig_1", "start": 136897, "stop": 137103, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGCTTTAATTGGTCAAATTGCCGTGGGAAAGAGTACTTTTTTAGAAAATTTTAAGAAATTAGGTCTTAAAACTTTTAATTGTGACGAGTTTATTAATCGTGAATATCAAAAAAATGGCAAAATTTACCACAAAATTAATCAAGAATTAGGTGATTTTTTAAATGATAAAAATGGTATAAGTAAGGAAAAAATTAAACTTTGA", "aa": "MIALIGQIAVGKSTFLENFKKLGLKTFNCDEFINREYQKNGKIYHKINQELGDFLNDKNGISKEKIKL", "aa_hexdigest": "efc83422adb763fcb088466d62bf4452", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7849.048899999999, "isoelectric_point": 9.336520957946778 }, "id": "OAIKOHHJFB_283", "locus": "GCA000209735_00251" }, { "type": "cds", "sequence": "contig_1", "start": 137125, "stop": 137469, "strand": "+", "frame": 1, "gene": "coaE", "product": "dephospho-CoA kinase", "db_xrefs": [ "EC:2.7.1.24", "GO:0004140", "GO:0005524", "GO:0005737", "GO:0015937", "KEGG:K00859", "SO:0001217", "UniRef:UniRef50_A0A3P8KM71", "UniRef:UniRef90_C4XE26" ], "nt": "TTGGGAGAATTGGAAAAAATCATTTATCCTTTAATTTTTGAAGAAATAAAAGATGGAAATTTTTTCATTGTTGAGATACCAAATTTATTGCCAAAAAATTTTAAATTTATTTCTTTGTTTTCTGCAGTTTTGTGCTTAGAAACTAGCCAAAAAAATAGGTTAAAAAATTTTAAAAAACGTAATGTGGATAAAACTAAAATTAAGGCAATAGATGAAAAAAACAACCCTTTTTCGATAAAAAATCAACTTTTTGGGTCTATACCAATTGTGGATTTCTATGGGCATAACTTCACTAATAATGAAAATATTTTAGATTTTTTAAAACTTCTATTTTTTATCTCATAA", "aa": "MGELEKIIYPLIFEEIKDGNFFIVEIPNLLPKNFKFISLFSAVLCLETSQKNRLKNFKKRNVDKTKIKAIDEKNNPFSIKNQLFGSIPIVDFYGHNFTNNENILDFLKLLFFIS", "aa_hexdigest": "ded5c8f640009da2b7fb50f435665ff9", "start_type": "TTG", "rbs_motif": null, "genes": [ "coaE" ], "truncated": "5-prime", "pseudogene": { "start": 136897, "stop": 137469, "observations": { "mutation": [ 137101 ], "stop": [ 137101 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE26", "query_cov": 1.0, "subject_cov": 0.6, "identity": 0.991, "score": 224.0, "evalue": 3.29e-73, "gene_start": 1, "gene_end": 114, "reference_start": 77, "reference_end": 190, "reference_sequence": "MIALIGQIAVGKSTFLENFKKLGLKTFNCDEFINREYQKNGKIYHKINQELGDFLNDKNGISKEKIKLWIDQKAHNLGELEKIIYPLIFEEIKDGNFFIVEIPNLLPKNFKFISLFSAVLCLETSQKNRLKNFKKRNVDKTKIKAIDEKNNPFSIKNQLFGSIPIVDFYGHNFTNNENILDFLKLLFFIS" }, "paralog": false, "identity": 0.9947368421052631, "score": 379.0, "evalue": 1.16e-136, "description": "Internal stop codon at 137101. Nonsense mutation around 137101.", "psc": { "uniref90_id": "UniRef90_C4XE26", "gene": "coaE", "product": "dephospho-CoA kinase", "ec_ids": [ "2.7.1.24" ], "uniref50_id": "UniRef50_A0A3P8KM71", "kegg_orthology_id": "K00859", "go_ids": [ "GO:0004140", "GO:0005524", "GO:0005737", "GO:0015937" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE26", "EC:2.7.1.24", "UniRef:UniRef50_A0A3P8KM71", "KEGG:K00859", "GO:0004140", "GO:0005524", "GO:0005737", "GO:0015937" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KM71", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KM71" ], "product": "Dephospho-CoA kinase" } }, "id": "OAIKOHHJFB_284", "locus": "GCA000209735_00252" }, { "type": "cds", "sequence": "contig_1", "start": 137519, "stop": 138481, "strand": "+", "frame": 2, "gene": "dnaD", "product": "DnaD domain-containing protein", "db_xrefs": [ "RefSeq:WP_013526636.1", "SO:0001217", "UniParc:UPI0001A8C3A8", "UniRef:UniRef100_C4XE27", "UniRef:UniRef50_N9V249", "UniRef:UniRef90_C4XE27" ], "nt": "ATGCTAAAAAATATTCCTTACGCATTCTTCAGAATCAAACATTCTTCATCAGTTAGTGGAGAAGATTTAAAAAACTTACGCAAGTTTTACGCTCCTATTTTAGGTAGTGATGCAATTTTGCTTTATGAATACCTTAGAGATCTATCATTTTCTCAATCTCCAGAAACTGGTTTTTATGACTATGATAGCCTTACTTATCTATTAAATATGGATGTAAATAAACTAAATCAAGCACGTATAAAATTAGAATCTGTATCTTTAATTTCTACTTTTATAGATGAGTTTAATCGCAAAACACTTTTTGTTATTGAAAAACCACTTGATAATACAGGTTTCAAAAAGAATTTAATTCTAGCAAACAAACTCATTAGTATTATTGGTAGCAAAAACTTTGAAAGATTAGTTGGACGTGAAAGAAATATTTATTTAAGTAAAGCTTCAAATCTTTTGGATGCTTCAGCTTCTTTTGAAGAAGTTTTTCCTGAACCAAGTGAAGAATTAACTATTACTTTTTCACATGAGGTTGACTCAAGTATGATTAAAACTCAAGAAATAGTTGAAGAAATTCAAACCAAATTGTCACTTAATACTTTTGATTATCCTAATCCTTATGAAGCAATACTTAAAACTGATTCAAGATACTTCTATTCTCAAATTAGTCACAAAATACCACGTCAAAGTGTTTTAGATTTAATTAAAGAAAGTAGAGCAGCTGGCTTAGGTGATCCTTGCTTAAACTTAGTATTCTTTTATTCAAATGAAATAAATAATTGCATAAATACCAATTATGTGGGTAAAATTATTAAGGATTTAATTAAAAAAGAAATCTTCTCATTTGAAGCAATTGAAAATTATTTAGATGACATACTTAAAGTTAAAAACAGAATTGCAGTTTCAAAAATTAATTTATATAAAGCTACCTATTTAGAATCACTTCAAAGAGAAGACAACAACAATATTTAA", "aa": "MLKNIPYAFFRIKHSSSVSGEDLKNLRKFYAPILGSDAILLYEYLRDLSFSQSPETGFYDYDSLTYLLNMDVNKLNQARIKLESVSLISTFIDEFNRKTLFVIEKPLDNTGFKKNLILANKLISIIGSKNFERLVGRERNIYLSKASNLLDASASFEEVFPEPSEELTITFSHEVDSSMIKTQEIVEEIQTKLSLNTFDYPNPYEAILKTDSRYFYSQISHKIPRQSVLDLIKESRAAGLGDPCLNLVFFYSNEINNCINTNYVGKIIKDLIKKEIFSFEAIENYLDDILKVKNRIAVSKINLYKATYLESLQREDNNNI", "aa_hexdigest": "baca658575854f61784cbbd66cb31001", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001A8C3A8", "ncbi_nrp_id": "WP_013526636.1", "uniref100_id": "UniRef100_C4XE27", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C3A8", "RefSeq:WP_013526636.1", "UniRef:UniRef100_C4XE27" ] }, "ips": { "uniref100_id": "UniRef100_C4XE27", "uniref90_id": "UniRef90_C4XE27" }, "psc": { "uniref90_id": "UniRef90_C4XE27", "product": "DnaD domain-containing protein", "uniref50_id": "UniRef50_N9V249" }, "pscc": { "uniref50_id": "UniRef50_N9V249", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9V249" ], "product": "DnaD domain-containing protein" }, "genes": [ "dnaD" ], "id": "OAIKOHHJFB_285", "locus": "GCA000209735_00253" }, { "type": "cds", "sequence": "contig_1", "start": 138495, "stop": 138800, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGCAAAAGAAGAAATTAAAAATGAGTTATTTTTTAATTCCAAGGCTGAATTTGAAAACAAGGCACTTGAAATAATGCATGGCCATCCGCAACTAGAAGCATTAATTCAAGCTAACAATATTACAAATGAAGAAATTTTGGAAAACTTATTTACATTTATTAATATTAAAGAATCATTGGATAATTCTCAAATTTATCCATGGATTTTTTCTATTTCAAGAAAAAATGAAAAATTAGTTTTTAAAAAGATTGCTGCAAAAAATGAATACAAATCAGTTGTTACACGACACATCAATTTATGA", "aa": "MAAKEEIKNELFFNSKAEFENKALEIMHGHPQLEALIQANNITNEEILENLFTFINIKESLDNSQIYPWIFSISRKNEKLVFKKIAAKNEYKSVVTRHINL", "aa_hexdigest": "389e0559edf23678371fb16b54db587f", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11823.435999999983, "isoelectric_point": 6.295850563049316 }, "id": "OAIKOHHJFB_286", "locus": "GCA000209735_00254" }, { "type": "cds", "sequence": "contig_1", "start": 138876, "stop": 139184, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00004.35", "PFAM:PF00308.24", "PFAM:PF01695.23" ], "nt": "ATGTTTGAATATATTAATCATCTAAAAGTTGCAATAGCTAAAAAGAAACAAAAAGGACTAAAAGGCATTTATGTCTATGGAGCTAACAACACTGGCAAAACTTACATTGCTAGTGCAATAGCCAACGAATTTGCAGCTGAAGGTATTTCATCAGTTTATTTAACAACCAGTGCTTTATATTCATTCTTAATTTCAAACATGGGTAGCAATGCTACAAATGCTAATGCAGTTATTGACATAATTAACAAACTCAAAAAAGTTAATGCATTAATTATTGACGAAATTGGTTTAGAAAAAAATAACTACTGA", "aa": "MFEYINHLKVAIAKKKQKGLKGIYVYGANNTGKTYIASAIANEFAAEGISSVYLTTSALYSFLISNMGSNATNANAVIDIINKLKKVNALIIDEIGLEKNNY", "aa_hexdigest": "cdc3287e94fa236ed8c6793449701b29", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00004.35", "name": "AAA", "length": 73, "aa_cov": 0.7156862745098039, "hmm_cov": 0.9041095890410958, "evalue": 4.624401300372532e-08, "score": 29.68245506286621, "start": 23, "stop": 95 }, { "id": "PF00308.24", "name": "Bac_DnaA", "length": 89, "aa_cov": 0.8529411764705882, "hmm_cov": 0.9438202247191011, "evalue": 8.362536249751732e-07, "score": 25.41262435913086, "start": 9, "stop": 95 }, { "id": "PF01695.23", "name": "IstB_IS21", "length": 76, "aa_cov": 0.7450980392156863, "hmm_cov": 0.9342105263157895, "evalue": 2.909561061923127e-08, "score": 29.561506271362305, "start": 23, "stop": 98 } ], "seq_stats": { "molecular_weight": 11094.677899999986, "isoelectric_point": 9.30196590423584 }, "id": "OAIKOHHJFB_287", "locus": "GCA000209735_00255" }, { "type": "cds", "sequence": "contig_1", "start": 139203, "stop": 139388, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGATAATCGAAAGCAGAATCGCAAATAATAAAATTACATTTTTCTTCAGTGGCATGAACAAAAAAGAATTTGAATCTTACTACCATGATCAACTTAAAAGAGAGCCTTACAAAGTTAAAACTTTTACTTCTCGAATTCTTAATAACACACTTGAATTTTGCATTGATGAAATTGAAAAATAA", "aa": "MEIIESRIANNKITFFFSGMNKKEFESYYHDQLKREPYKVKTFTSRILNNTLEFCIDEIEK", "aa_hexdigest": "af2a623284fc58496cd29cefca5b5ed6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7429.4203, "isoelectric_point": 6.563391685485839 }, "id": "OAIKOHHJFB_288", "locus": "GCA000209735_00256" }, { "type": "cds", "sequence": "contig_1", "start": 140328, "stop": 140513, "strand": "+", "frame": 3, "gene": null, "product": "IS30 family transposase", "db_xrefs": [ "GO:0004803", "SO:0001217", "UniRef:UniRef50_UPI002805CAB8", "UniRef:UniRef90_UPI000F044D4B" ], "nt": "ATGGACTCAAATGAAAATTTCAATGGTTTAGTTAGAAGATTTTTTAAGAAAAAAACTAATTTTGATGATATTAATGAAGAAGAAACTCTAAAAGTACAAAATGAAATAAACAATCGTCCAAGAGAAATTTTTGGATATTTATCTGCTTTGGAAATGTACGAAAAATTGAATGCAGAAAATCCTTGA", "aa": "MDSNENFNGLVRRFFKKKTNFDDINEEETLKVQNEINNRPREIFGYLSALEMYEKLNAENP", "aa_hexdigest": "46b18a51e020676b557ee352632478bd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 140124, "stop": 140564, "observations": { "mutation": [ 140169, 140202, 140511 ], "stop": [ 140169, 140202, 140511 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000F044D4B", "query_cov": 1.0, "subject_cov": 0.4178082191780822, "identity": 0.9840000000000001, "score": 119.0, "evalue": 4.51e-33, "gene_start": 1, "gene_end": 61, "reference_start": 69, "reference_end": 129, "reference_sequence": "ISRYGFLVKIPNKNPWNLNLYLWNLIWKHKLNVKSITQDNGLEFSTLFFIGYKLKIFIYKADPYASFQMDSNENFNGLVRRFFKKKTNFDDINEEEILKVQNEINNRPREIFGYLSALEMYEKLNAENPWNSTGIDKVLYGKQKRN" }, "paralog": false, "identity": 0.958904109589041, "score": 283.0, "evalue": 2.64e-100, "description": "Internal stop codon at 140169, 140202, 140511. Nonsense mutation around 140169, 140202, 140511.", "psc": { "uniref90_id": "UniRef90_UPI000F044D4B", "product": "IS30 family transposase", "uniref50_id": "UniRef50_UPI002805CAB8", "go_ids": [ "GO:0004803" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000F044D4B", "UniRef:UniRef50_UPI002805CAB8", "GO:0004803" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002805CAB8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002805CAB8" ], "product": "IS30 family transposase" } }, "id": "OAIKOHHJFB_289", "locus": "GCA000209735_00257" }, { "type": "cds", "sequence": "contig_1", "start": 140843, "stop": 141358, "strand": "-", "frame": 1, "gene": "apt", "product": "adenine phosphoribosyltransferase", "db_xrefs": [ "EC:2.4.2.7", "GO:0002055", "GO:0003999", "GO:0005737", "GO:0006166", "GO:0006168", "GO:0009116", "GO:0016208", "GO:0044209", "KEGG:K00759", "RefSeq:WP_013526633.1", "SO:0001217", "UniParc:UPI0000125CDD", "UniRef:UniRef100_Q9RFQ2", "UniRef:UniRef50_Q9RFQ2", "UniRef:UniRef90_Q9RFQ2" ], "nt": "ATGGATTTAAAAAAATATATTCGAGATATTGAAAATTTTCCTAAAACAGGCATAATGTTTAAAGATATTTCACCATTATTGGCAAATGGTAAAGCATTAAATTATACAATCACAGAAATGGCAAAATTAGCTCAAGATGTTGATGTTATTGTTGGACCAGATGCGAGAGGATTTTTATTTGGCACGCCTACTGCAGCATTCTTGAAGAAACCATTTATTATGGTTAGAAAACCAGGAAAATTACCTGGTGATGTTATTTCAAAAAGTTACGATTTGGAATATGGAAATAATGTTTTACAAATTCAAAAAGGGTTTATTAAAAAAGGTCAAACAGTTGCAATTATTGATGATGTTTTAGCAACGGGTGGAACAACTAAAGCTATTATAAAACTATTAGAAGAACAAGGTGCAATTGTTAAAAAAGTTATTTTACTTCTTGAATTAGTAGATCTTAATGGTAGACAATTAATCACTAAAGATAATGATATTGAAATAGTTTCCTTAGTTAAATTTTAA", "aa": "MDLKKYIRDIENFPKTGIMFKDISPLLANGKALNYTITEMAKLAQDVDVIVGPDARGFLFGTPTAAFLKKPFIMVRKPGKLPGDVISKSYDLEYGNNVLQIQKGFIKKGQTVAIIDDVLATGGTTKAIIKLLEEQGAIVKKVILLLELVDLNGRQLITKDNDIEIVSLVKF", "aa_hexdigest": "4bb0073cef756d6de0022d2f5d20bfd0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0000125CDD", "ncbi_nrp_id": "WP_013526633.1", "uniref100_id": "UniRef100_Q9RFQ2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000125CDD", "RefSeq:WP_013526633.1", "UniRef:UniRef100_Q9RFQ2" ] }, "ips": { "uniref100_id": "UniRef100_Q9RFQ2", "uniref90_id": "UniRef90_Q9RFQ2" }, "psc": { "uniref90_id": "UniRef90_Q9RFQ2", "gene": "apt", "product": "adenine phosphoribosyltransferase", "ec_ids": [ "2.4.2.7" ], "uniref50_id": "UniRef50_Q9RFQ2", "kegg_orthology_id": "K00759", "go_ids": [ "GO:0002055", "GO:0003999", "GO:0005737", "GO:0006166", "GO:0006168", "GO:0009116", "GO:0016208", "GO:0044209" ] }, "pscc": { "uniref50_id": "UniRef50_Q9RFQ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9RFQ2" ], "product": "Adenine phosphoribosyltransferase" }, "genes": [ "apt" ], "id": "OAIKOHHJFB_290", "locus": "GCA000209735_00258" }, { "type": "cds", "sequence": "contig_1", "start": 141388, "stop": 143025, "strand": "-", "frame": 2, "gene": "infB", "product": "translation initiation factor IF-2", "db_xrefs": [ "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006413", "SO:0001217", "UniRef:UniRef50_Q98R05", "UniRef:UniRef90_A0A3P8LI10" ], "nt": "ATGAAAGGAAAGCTTTATCAAATTAACTACGTACTTGATGAAGAAGAAATTGCTGAAGTATGTATGGAAAGAGGACTTGACTTTAGAAAAGAACAAAACATTGATGCTGCAAATTTTTTGCATGCAGTTAAATTTGAAGATGATGAAAAATCATTAGTAAAAAGACCTCCAATTATTACAGTTATGGGTCATGTTGATCACGGAAAAACTACATTAATTGACTTTATTAGAAAAACTAAAGTTGCTGAAACTGAAAGTAGTGGTATTACTCAACATACTGGTGCTTACCAAGTAGTTTACAAAGGCAACAAAATTACATTTATTGACACTCCTGGACATGAAGCATTTACTGAAATGCGTTCACGTGGAGCTAAAATTACAGACATTATTATTTTAGTAGTAGCTGCTGATGATGGTGTTATGCCACAAACTAGAGAAGCTATTGACCACGCAAAAGCAGCTGATGTTCCTATCGTTGTTTTTGTAAATAAAATAGATAAACCAAATAAGGATCTTGAAAGAATTAAAAGAGAATTAGCTGAATGCAACGTTATTGTTGAAGAATATGGTGGTGACGTTCAAATGGTAACTGGTTCTGCTTTAAAAGGTCAAGGTATTGAAGATTTATTTGAAGCAATTAGTTTACTTGCTCAATTATTGGATTTAAAAGCCAACCGTTCAAGATATCCAGTTGGTACTGTTATTGAAAGTAAAATAGATAAAGGTGTTGGTGTTGTTTCAACTTTAATTGTTGAAAACGGTAGCTTATATAAAGGAGACTTTATTGTAGCTGGCTCAAGTTATGGGCGTGTGCGTTCATTAAAAGATGCTACTGGAAAACAAATGGAACATGCTCACCCTGGAACACCAGTTGTTGTTACTGGATTAAATAATTCACCACTTGCAGGTGATAGATTTATTGGTTTTCAAGATGAAAAATTTGCTAAAAAATTAGCTGAAGAAAAAGCTCAAATCGATAAGAACAGAGCTTTATATGACAAGAGCCAAACTTCAGAAATTGAAATTGGCAAAAAGGTATTTAATGTAATCATTAGATCAGATGTTCAAGGAACAGCTGAAGCTATCAAAGGCAAAATAGATGGCATGTGTAATGATGAAGCAATGATTAAAGTTATTGGCGCTCAAGCGGGACAAATAACAAATGCTGACTTATTATTAGCTCAAGCCTCAGATGCAAGGATTATAACTTTCAATGTTAAGCCATCTGCAGCTATTAAGCAAAGTGCAAAACATTCGGGAATTACAATTGTTAATTATTCTGTTATTTACAAAATTATTGAAGATATGGAAGCTCACCTTAAGGGTCAAAAAGCAGTTGTTTATGAAGAAAAGAAAATCGGATCAGCTCATATTATCAAAGTTTTCTTCTATTCAAAAGTTGGAGCAATTGCTGGATGTATGATGGATGAAGGCGTTGTAAAAGCTAATTGTAAAGTTAAAGTTTTTAGAGGCAGAAAAATGATTCATGATGGTGTAATTGAAACACTTAAACGTGAATTAAATGATTCAAAAGAAGTTGAGAAAGGTAAAGATTTTGGTACTCACATTAAGAAGTTTGATGATATCAAGGAAGATGATATTTTAGAATTCTTTGAAGATGTTCCAGTATCTTTATAA", "aa": "MKGKLYQINYVLDEEEIAEVCMERGLDFRKEQNIDAANFLHAVKFEDDEKSLVKRPPIITVMGHVDHGKTTLIDFIRKTKVAETESSGITQHTGAYQVVYKGNKITFIDTPGHEAFTEMRSRGAKITDIIILVVAADDGVMPQTREAIDHAKAADVPIVVFVNKIDKPNKDLERIKRELAECNVIVEEYGGDVQMVTGSALKGQGIEDLFEAISLLAQLLDLKANRSRYPVGTVIESKIDKGVGVVSTLIVENGSLYKGDFIVAGSSYGRVRSLKDATGKQMEHAHPGTPVVVTGLNNSPLAGDRFIGFQDEKFAKKLAEEKAQIDKNRALYDKSQTSEIEIGKKVFNVIIRSDVQGTAEAIKGKIDGMCNDEAMIKVIGAQAGQITNADLLLAQASDARIITFNVKPSAAIKQSAKHSGITIVNYSVIYKIIEDMEAHLKGQKAVVYEEKKIGSAHIIKVFFYSKVGAIAGCMMDEGVVKANCKVKVFRGRKMIHDGVIETLKRELNDSKEVEKGKDFGTHIKKFDDIKEDDILEFFEDVPVSL", "aa_hexdigest": "55a4826e0a8eda6a849cfc1cc2bdce66", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3P8LI10", "query_cov": 1.0, "subject_cov": 0.9068219633943427, "identity": 1.0, "score": 1055.0, "evalue": 0.0, "valid": true, "gene": "infB", "product": "translation initiation factor IF-2", "uniref50_id": "UniRef50_Q98R05", "go_ids": [ "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_Q98R05", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98R05" ], "product": "Translation initiation factor IF-2" }, "genes": [ "infB" ], "id": "OAIKOHHJFB_291", "locus": "GCA000209735_00259" }, { "type": "cds", "sequence": "contig_1", "start": 143032, "stop": 143193, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAGAAAGAACAGAATAAGTAACGTTGATGAAGTCAAGAACCAACTTTCAGATATTAAAACTGAAGTTAAGAATGGTGTTTTCATTTTTACAAACAAAATGCCATTAGGAGAATTTGCACAAAAAATTGGTATGAATGCCAATGATTTGATCAAGTGA", "aa": "MPRKNRISNVDEVKNQLSDIKTEVKNGVFIFTNKMPLGEFAQKIGMNANDLIK", "aa_hexdigest": "3364c9d5629f3127b5f868b7a157eec0", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6035.9703, "isoelectric_point": 9.600390052795408 }, "id": "OAIKOHHJFB_292", "locus": "GCA000209735_00260" }, { "type": "cds", "sequence": "contig_1", "start": 143467, "stop": 143733, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATTCATTACAAGAAGATGACTCAAACTTCGAAGAATTAGTTAAGAAAATTGAAAGCGAATATGCAGTTGAAAATGAAGTAGAAAACATTAAAAATGTTGAAGTAACAAAACCAGTTGCTCAAGATAACAAAATTTCCGCATCTGACTATAAAAAAGCTAAAGAAGTCGCAAAAGACTTTGTTGTTGATGATGATTTAAAGAATTTTGGAATTGACGATTTTGATTTAAGTGACATTGATTTTGATGAAGAAGAAAACAAATAA", "aa": "MDSLQEDDSNFEELVKKIESEYAVENEVENIKNVEVTKPVAQDNKISASDYKKAKEVAKDFVVDDDLKNFGIDDFDLSDIDFDEEENK", "aa_hexdigest": "1994556343f9dcef3bd3cc6ad8fea579", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10124.812699999997, "isoelectric_point": 4.052472496032714 }, "id": "OAIKOHHJFB_293", "locus": "GCA000209735_00261" }, { "type": "cds", "sequence": "contig_1", "start": 144376, "stop": 144534, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAACAGTAATGTTAATGCATATTTACCAGCAGGATTAATTAGTACAAAAAGAAAAGCAAACCCAGGTCAATATTTTGATGTTGTTATTGAAAAAGTTTCAGAAGAAACAAAATTGAGTCAAATTGAAGTTTCATTAGACTCACCTAAAATTGTTTGA", "aa": "MNSNVNAYLPAGLISTKRKANPGQYFDVVIEKVSEETKLSQIEVSLDSPKIV", "aa_hexdigest": "2af832be75027ba1af4df14ab9309e20", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5722.4785999999995, "isoelectric_point": 6.090263938903808 }, "id": "OAIKOHHJFB_294", "locus": "GCA000209735_00262" }, { "type": "cds", "sequence": "contig_1", "start": 144736, "stop": 145017, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08529.16" ], "nt": "TTGATAGGTTATTTTGAAAAAGAAAAACTTCATATTGAAGAAGTTATCAAAATTTTTTCAGAAGAAACTACACGAATTATTAACAAAATCTTAGATCCAAATGCTGAAATAGTTTTTGAATTAGACAAAGAAAAACAAGAAGTTCACATTTTTAACAACAAAACATGTGTAACTAAAGATGAAGAATTATTGCTTGATCTTGAAAATCAAATAAGTTTTGTTGGATATAAAGAAGCAAAAGTAAGAATGCCTAAAGTTAAAGAAGATGATGAAATTAAATGA", "aa": "MIGYFEKEKLHIEEVIKIFSEETTRIINKILDPNAEIVFELDKEKQEVHIFNNKTCVTKDEELLLDLENQISFVGYKEAKVRMPKVKEDDEIK", "aa_hexdigest": "9b49a6bdd72f0a512a7393ca428cc9df", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08529.16", "name": "NusA_N", "length": 88, "aa_cov": 0.9354838709677419, "hmm_cov": 0.9204545454545454, "evalue": 7.620471921598306e-07, "score": 25.491579055786133, "start": 6, "stop": 92 } ], "seq_stats": { "molecular_weight": 11041.588099999995, "isoelectric_point": 4.879707145690918 }, "id": "OAIKOHHJFB_295", "locus": "GCA000209735_00263" }, { "type": "cds", "sequence": "contig_1", "start": 145174, "stop": 145446, "strand": "-", "frame": 2, "gene": "rimP", "product": "Ribosome maturation factor RimP", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE34", "UniRef:UniRef90_C4XE34" ], "nt": "TTGTTAGACATTAGTGTCAATTATCATGACTTAAATAAGGTTGAAGAAATATCAAAACAACTTAATGAATTTATAGATTCTAATTATCCATCTTTGGATATTGATTCATTATCAATACATTCTCCAGGTGTCGAATTAACCTATAAAACAGATGAATTAGGAAAACACATTGGAGAAATGTTAACTATAAAATTAAATAAAAGTATTGAAAAACAAGACAAATTTAATGGTAAATTATTAGAAGTTTTAGAAGGCAAAATTCAAATAAGTTGA", "aa": "MLDISVNYHDLNKVEEISKQLNEFIDSNYPSLDIDSLSIHSPGVELTYKTDELGKHIGEMLTIKLNKSIEKQDKFNGKLLEVLEGKIQIS", "aa_hexdigest": "91f3260c4d36865ff043a97367d2ffa2", "start_type": "TTG", "rbs_motif": "AGxAG", "genes": [ "rimP" ], "truncated": "both", "pseudogene": { "start": 145093, "stop": 145725, "observations": { "mutation": [ 145176, 145521, 145581, 145602 ], "stop": [ 145176, 145521, 145581, 145602 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE34", "query_cov": 1.0, "subject_cov": 0.42857142857142855, "identity": 0.9890000000000001, "score": 173.0, "evalue": 3.89e-53, "gene_start": 1, "gene_end": 90, "reference_start": 94, "reference_end": 183, "reference_sequence": "MQLLKTVMRKLNVEKEKATTTKKNNFKTCTSASFFVDLKILWYNKTIKWILQSCNLQLFVIFVGGFMNWKEILEKQFGSQILEAKIIEEDDLKLLDISVNYHDLNKVEEISKQLNEFIDSNYPSLDIDSLSIHSPGVELTYKTDELGKHIGEMLTIKLNKSIEKQDKFNGKLLEVLEGKIQISWNNKGQFRKVFIDKNNIVKVEKYIKFN" }, "paralog": false, "identity": 0.9809523809523809, "score": 397.0, "evalue": 4.64e-144, "description": "Internal stop codon at 145176, 145521, 145581, 145602. Nonsense mutation around 145176, 145521, 145581, 145602.", "psc": { "uniref90_id": "UniRef90_C4XE34", "product": "Ribosome maturation factor RimP", "uniref50_id": "UniRef50_C4XE34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE34", "UniRef:UniRef50_C4XE34" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE34" ], "product": "Ribosome maturation factor RimP" } }, "id": "OAIKOHHJFB_296", "locus": "GCA000209735_00264" }, { "type": "cds", "sequence": "contig_1", "start": 145784, "stop": 146008, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAATAATTTAAAAACAGTTGGTAAAGTTGCTTATACAACTATTGATTTAAAAACAACAAAAGCTTTAGGCAAAAAACCAAATCAAGTTGTTAGAGTAAATACAATTGGGAATATTATTGGTTATCCATTTTGGGTTCAATTCAATGAAGAAGAAAATGGCAATGTTAGAATTGAATTTAGATCAAGTGGTCCTTGTGTTAGAAATGTTGCAGTTAAATGA", "aa": "MLNNLKTVGKVAYTTIDLKTTKALGKKPNQVVRVNTIGNIIGYPFWVQFNEEENGNVRIEFRSSGPCVRNVAVK", "aa_hexdigest": "2970673e9479e747f725cac31bca1e1e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8280.499499999998, "isoelectric_point": 9.917961311340331 }, "id": "OAIKOHHJFB_297", "locus": "GCA000209735_00265" }, { "type": "cds", "sequence": "contig_1", "start": 146264, "stop": 146647, "strand": "-", "frame": 1, "gene": "nrnA", "product": "bifunctional oligoribonuclease/PAP phosphatase NrnA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C6B900" ], "nt": "ATGGGACACATGAAAATTGGTAGCTTAAAAGAAGCAACAAAATTAATTAAAAAATATGACTCAATAGTTATTTTTCATCACATTAGACCAGATGGTGACTGCTTAGGATCACAAGCTGGTTTAGCAGAATTAATTAGAGCTAATTTTCCAGAAAAGAAAGTTTACACAGTTGGCGATAATGAAGGTATGTTTCCATTTTTAAATCTTAAATATGATAAATGTCCTTCAGATAATGTACTTAAAAAATCTTTAGGCATTATTCCGGATGCAAATGGTAAAGAAAGAATTCAATGTCGAGAAATTTTAGATAAAAACTTATTTGCTGAAACACTTAGAATTGATCATCATCCAAATGGTGATGACTTAGATAAATGTACCAGATGA", "aa": "MGHMKIGSLKEATKLIKKYDSIVIFHHIRPDGDCLGSQAGLAELIRANFPEKKVYTVGDNEGMFPFLNLKYDKCPSDNVLKKSLGIIPDANGKERIQCREILDKNLFAETLRIDHHPNGDDLDKCTR", "aa_hexdigest": "1ef3fc7e774a6e89631548d43958c6ca", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0024C6B900", "query_cov": 0.984251968503937, "subject_cov": 0.8741258741258742, "identity": 0.6559999999999999, "score": 175.0, "evalue": 3.75e-54, "valid": false, "product": "bifunctional oligoribonuclease/PAP phosphatase NrnA", "uniref50_id": "UniRef50_UPI0024C6B900" }, "pscc": { "uniref50_id": "UniRef50_UPI0024C6B900", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C6B900" ], "product": "bifunctional oligoribonuclease/PAP phosphatase NrnA" }, "genes": [ "nrnA" ], "id": "OAIKOHHJFB_298", "locus": "GCA000209735_00266" }, { "type": "cds", "sequence": "contig_1", "start": 147181, "stop": 147615, "strand": "-", "frame": 2, "gene": null, "product": "DHH family phosphoesterase", "db_xrefs": [ "EC:3.1.13.3", "EC:3.1.3.7", "KEGG:K06881", "SO:0001217", "UniRef:UniRef50_A0A449B7A5", "UniRef:UniRef90_C4XE36" ], "nt": "ATGAAAATAGGAAATTATAAAGTTGCATTACAAGCAATCGAAAAATATAAGAATATTATTATTTTTCATCACATTAGACCAGATGGTGACTGCTTAGGATCACAAGCTGGTTTAGCAGAATTAATAAAAAAGAATTATCCAAAGAAAAAAGTTTTTACAGTTGGAGATAATGTTCATACTTTTGATTTTATGAGTTACCACTATGACCCAATTGAAACGGTTGATTTTAAAAATTCATTAGGTATTGTAGTTGATGCTTCAAGTAGTGATCGGATTCAAGATGGACATTTATTAGTAGAAAAAAAATGTACAGCTGCTTTAAGAATTGATCACCATCCAAATGGTTCAGATATCGAATATGATTATTTATGGATCGATGAACACTATGTAGCTGCGGCTGAAATGGTGGCTATGTTAGCTAAAAGTGCAAAATGA", "aa": "MKIGNYKVALQAIEKYKNIIIFHHIRPDGDCLGSQAGLAELIKKNYPKKKVFTVGDNVHTFDFMSYHYDPIETVDFKNSLGIVVDASSSDRIQDGHLLVEKKCTAALRIDHHPNGSDIEYDYLWIDEHYVAAAEMVAMLAKSAK", "aa_hexdigest": "323886bd708bbedc1b7ebd8def08d762", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 146878, "stop": 147642, "observations": { "mutation": [ 147042, 147183 ], "stop": [ 147042, 147183 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE36", "query_cov": 1.0, "subject_cov": 0.4350453172205438, "identity": 1.0, "score": 297.0, "evalue": 2.09e-99, "gene_start": 1, "gene_end": 144, "reference_start": 10, "reference_end": 153, "reference_sequence": "MNIKLKEKNMKIGNYKVALQAIEKYKNIIIFHHIRPDGDCLGSQAGLAELIKKNYPKKKVFTVGDNVHTFDFMSYHYDPIETVDFKNSLGIVVDASSSDRIQDGHLLVEKKCTAALRIDHHPNGSDIEYDYLWIDEHYVAAAEMVAMLAKSAKWEITKKAAEHIYLGINTDSGRFLYPDTSARTYKLVAHLIEKGGLDSQWILRELNKRSSRDIKYTGEILSGFKQSGRVLYFEVTKEIMDKYGMNDLEAAIFVNELANIDDNSCWAFFIQLADGTVRCRLRSNGPLVNNVARLHNGGGHDNAAGCSINSWDEVPVVLKELNDCIKEWEAK" }, "paralog": false, "identity": 0.9881889763779528, "score": 503.0, "evalue": 8.16e-183, "description": "Internal stop codon at 147042, 147183. Nonsense mutation around 147042, 147183.", "psc": { "uniref90_id": "UniRef90_C4XE36", "product": "DHH family phosphoesterase", "ec_ids": [ "3.1.13.3", "3.1.3.7" ], "uniref50_id": "UniRef50_A0A449B7A5", "kegg_orthology_id": "K06881", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE36", "EC:3.1.13.3", "EC:3.1.3.7", "UniRef:UniRef50_A0A449B7A5", "KEGG:K06881" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449B7A5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B7A5" ], "product": "Bifunctional oligoribonuclease and PAP phosphatase nrnA" } }, "id": "OAIKOHHJFB_299", "locus": "GCA000209735_00267" }, { "type": "tRNA", "sequence": "contig_1", "start": 147799, "stop": 147875, "strand": "-", "gene": "trnH", "product": "tRNA-His(gtg)", "amino_acid": "His", "anti_codon": "gtg", "score": 71.4, "nt": "GCGGATGTGGTGAAGTGGTCTAACACAGCGGGTTGTGGTCCCGTCATTCGAGGGTTCGAATCCCTTCATCCGCCCCA", "db_xrefs": [ "SO:0000262" ], "anti_codon_pos": [ 147867, 147869 ], "id": "OAIKOHHJFB_26", "locus": "GCA000209735_00268" }, { "type": "cds", "sequence": "contig_1", "start": 147938, "stop": 149791, "strand": "+", "frame": 2, "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "db_xrefs": [ "GO:0002098", "GO:0008033", "KEGG:K03495", "RefSeq:WP_013526627.1", "SO:0001217", "UniParc:UPI0001F59AAE", "UniRef:UniRef100_C4XE37", "UniRef:UniRef50_Q98QV8", "UniRef:UniRef90_C4XE37" ], "nt": "ATGAATAAGAAAAGTAATAATGTACATAAATATGATGCTGTTGTTATAGGTGGAGGGCACGCTGGTTTAGAAGCTGCTTTTGCCTTAGCACACAAAAATCATAAAACTGTTTTGATATCTTTAAATAAGGAACGTTTAGGTATGATGCCATGTAATCCTTCAATTGGTGGACCAGCCAAAGGAATCATAACTAGAGAAATAGATGCCTTAGGCGGAATGCAAGGACTTTGGGCTGATTTAAGCATGATCCAATTGAAAATGCTTAATCAATCAAAAGGTCCTGCAGTTAGAGCACTTAGAGCTCAAATTGACAAAGAAAAATACATGAAGATAGCTTTAGAATACGTGTTAAAAGAACCTAACTTAGATCTTATTGAAGATATAGCTGAAGAAATATTAGTAAATAGTAAAAAAGAATTTAAAGGAATTAAACTAGCTAATGGCGATATAGTTGAAGCTGAGGTCTGTGTTGTAACTACTGGAACATATATGGACAGTAGAATTCTTAGAGGAGATGTTATTAAAATCAGTGGACCTGATAATGAAAAAACTACACCTAAATTAAGTGCTTCATTAAAAGAACATGGTTTTATAATTCAAAGACTTAAAACTGGAACACCTCCAAGAATCTTTTCAGATTCAATTGACTACTCGCAAGTAGAACAAGAAGTATTAAGTGATACTAACTTATCATTTTCATCTAGATCAAATGTAAAATTACCTCGTCAAATTGCTTGTTACTTAACTTATACAACTCCTGAAACTCACAAAATAATAGAAGAAAATTTAACTAAAAGTGCTATGTATGGAGGAATAATCAAAGGTATTGGCCCTCGTTATTGTCCTTCAGTTGAAGACAAAATTGTTAAGTTTCCTTCAAAAGAAAGACATCAAATTTTCTTCGAACCTGAAACTGCTGATGGTTCAATTACTTATGTTAATGGGCTCTCTACTTCAATGCCAGTTGAAGTCCAAGAACTAATGATTAGAAGTATTCCTGGTCTTCAAAAATGTAAGATTCAAAAGTATGCTTATGCTATTGAATATGATGCAATTGATCCTTTGCAATTAAAACCTACTTTAGAAACTAAAATCATTAAAAATTTTTATACTGCAGGTCAAATTAATGGAACTAGTGGTTACGAAGAGGCTGCTGCTCAAGGTTTAATTGCAGGTATTAATGCAGCCTTAAACTTAGAAAAAAAAGATGGTATAGTTATTTTAAGAAACCATGCTTATATAGGAGTTTTAATTGATGATTTGGTTACTAAAGGAACTAAAGAACCTTATAGAATGCTAACTTCAAGAGCTGAATATAGATTACTTTTAAGAAATGATAATGCTGATATTCGTTTAGCCGAATATGGCTATAAAATAGGCTTAATTGACAAACATCAATATCAAAAGGTTATTAAAAAATATGAATTAATTGACCAAGAAATTCAAAGACTTCATACTACTTATCTTTCTTCAAAAGATCCTGTGGCTATAAAATATGGAATCACAACAGGTATCTCATTATTACAAACTTTAGCTCGTCCTGCAGTTGATCCTTATGATATCATTCCTGATTTTCCATACCTAGAAGAATTAACAACAATGGTTAGACTTCATGGTTATATTGAAAAACAAAAAAGTGATGCTAATAAAGCTGTAAGACTAGAAAATTACAAAATACCAGAAGACTTGGACTACAACAAAATTAATAATATTGCTACAGAAGCTAAACAAAAATTAATCAAAGTTAAACCAACAACAATCGGCCAAGCAAGTAGAATCAGTGGCATTAATCCAGCTGATATTCAAATGCTTATGTTTTATTTAGAAACTACAAGAAAAAAGAATAATGCATAA", "aa": "MNKKSNNVHKYDAVVIGGGHAGLEAAFALAHKNHKTVLISLNKERLGMMPCNPSIGGPAKGIITREIDALGGMQGLWADLSMIQLKMLNQSKGPAVRALRAQIDKEKYMKIALEYVLKEPNLDLIEDIAEEILVNSKKEFKGIKLANGDIVEAEVCVVTTGTYMDSRILRGDVIKISGPDNEKTTPKLSASLKEHGFIIQRLKTGTPPRIFSDSIDYSQVEQEVLSDTNLSFSSRSNVKLPRQIACYLTYTTPETHKIIEENLTKSAMYGGIIKGIGPRYCPSVEDKIVKFPSKERHQIFFEPETADGSITYVNGLSTSMPVEVQELMIRSIPGLQKCKIQKYAYAIEYDAIDPLQLKPTLETKIIKNFYTAGQINGTSGYEEAAAQGLIAGINAALNLEKKDGIVILRNHAYIGVLIDDLVTKGTKEPYRMLTSRAEYRLLLRNDNADIRLAEYGYKIGLIDKHQYQKVIKKYELIDQEIQRLHTTYLSSKDPVAIKYGITTGISLLQTLARPAVDPYDIIPDFPYLEELTTMVRLHGYIEKQKSDANKAVRLENYKIPEDLDYNKINNIATEAKQKLIKVKPTTIGQASRISGINPADIQMLMFYLETTRKKNNA", "aa_hexdigest": "23272e9ffd7deed5b575b5c56c0a4d03", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59AAE", "ncbi_nrp_id": "WP_013526627.1", "uniref100_id": "UniRef100_C4XE37", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59AAE", "RefSeq:WP_013526627.1", "UniRef:UniRef100_C4XE37" ] }, "ips": { "uniref100_id": "UniRef100_C4XE37", "uniref90_id": "UniRef90_C4XE37" }, "psc": { "uniref90_id": "UniRef90_C4XE37", "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "uniref50_id": "UniRef50_Q98QV8", "kegg_orthology_id": "K03495", "go_ids": [ "GO:0002098", "GO:0008033" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QV8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QV8" ], "product": "tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG" }, "genes": [ "mnmG" ], "id": "OAIKOHHJFB_300", "locus": "GCA000209735_00269" }, { "type": "cds", "sequence": "contig_1", "start": 149784, "stop": 150230, "strand": "+", "frame": 3, "gene": null, "product": "Ribosomal RNA large subunit methyltransferase H", "db_xrefs": [ "EC:2.1.1.177", "KEGG:K00783", "RefSeq:WP_013354334.1", "SO:0001217", "UniParc:UPI0001E32F40", "UniRef:UniRef100_C4XE38", "UniRef:UniRef50_Q98QV7", "UniRef:UniRef90_C4XE38" ], "nt": "ATGCATAAGATTAAAATAATTGCAGTTGGTTCACTTAGTCCAGAATTTAAAGTTTTATTTAATCAATATGCTAAAAACATAAGTCATTTTGCTGAATTAAATGTTGTTGAAGTAAAAGAATTGAGTGAGGAAAAAAATATCGAAGTCAAGAAAGACAAGGAAACAAAATTAATTAATTCTTATGTTGATAATAATAGTTTTGTTATTTATGCTTCATTAAAAGGAAAAAGTTTAGATTCAGTTGAATTAAGCGAAATAGTTAATAACAATGTTAATTTAACCTTTATAATTGGCGGCAGTGATGGAGTTAATGAAGAATTAATTAAATGCAATATGAAAATTAATTCTTCAAAATTAACTTGGCCACATCAATTGTTTAGAGTTATGTTAGCTGAACAAATCTATCGTTCATTTATGATCATTAATAATAAAAAGTATCATAAATAA", "aa": "MHKIKIIAVGSLSPEFKVLFNQYAKNISHFAELNVVEVKELSEEKNIEVKKDKETKLINSYVDNNSFVIYASLKGKSLDSVELSEIVNNNVNLTFIIGGSDGVNEELIKCNMKINSSKLTWPHQLFRVMLAEQIYRSFMIINNKKYHK", "aa_hexdigest": "5a24f8abde1e52ce51a1ab84206930eb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32F40", "ncbi_nrp_id": "WP_013354334.1", "uniref100_id": "UniRef100_C4XE38", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F40", "RefSeq:WP_013354334.1", "UniRef:UniRef100_C4XE38" ] }, "ips": { "uniref100_id": "UniRef100_C4XE38", "uniref90_id": "UniRef90_C4XE38" }, "psc": { "uniref90_id": "UniRef90_C4XE38", "product": "Ribosomal RNA large subunit methyltransferase H", "ec_ids": [ "2.1.1.177" ], "uniref50_id": "UniRef50_Q98QV7", "kegg_orthology_id": "K00783" }, "pscc": { "uniref50_id": "UniRef50_Q98QV7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QV7" ], "product": "Ribosomal RNA large subunit methyltransferase H" }, "genes": [], "id": "OAIKOHHJFB_301", "locus": "GCA000209735_00270" }, { "type": "cds", "sequence": "contig_1", "start": 150265, "stop": 150459, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE39", "UniRef:UniRef90_C4XE39" ], "nt": "ATGTCTGAAAAAGGAAAAAAATTTAAAAAGAAACGTACAGGAATGATAATTGCTAGTTTAATATTTCTTTTTATAAGTATTGGATTAGCTGGAGCATTATTTCCAATAATTCATATAGTTGTTACAACTAAAAATTCAATTGGTTCAATAATAGCCATTATCATATCAGCTGTTTTTTTCATAATAACAATTTGA", "aa": "MSEKGKKFKKKRTGMIIASLIFLFISIGLAGALFPIIHIVVTTKNSIGSIIAIIISAVFFIITI", "aa_hexdigest": "9d77ab5e6f8731718071735c0a82944e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 150265, "stop": 150705, "observations": { "mutation": [ 150457, 150658 ], "stop": [ 150457, 150658 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE39", "query_cov": 1.0, "subject_cov": 0.4383561643835616, "identity": 1.0, "score": 108.0, "evalue": 6.46e-29, "gene_start": 1, "gene_end": 64, "reference_start": 1, "reference_end": 64, "reference_sequence": "MSEKGKKFKKKRTGMIIASLIFLFISIGLAGALFPIIHIVVTTKNSIGSIIAIIISAVFFIITIWFINFSIGTNYKVVNYKDKTIEVLVGVSYIELLVDGKTVDKLNVFKKRNASLSHNLDGDLIHVRIDGWVFYQVNIKINNELL" }, "paralog": false, "identity": 0.9863013698630136, "score": 273.0, "evalue": 1.87e-96, "description": "Internal stop codon at 150457, 150658. Nonsense mutation around 150457, 150658.", "psc": { "uniref90_id": "UniRef90_C4XE39", "uniref50_id": "UniRef50_C4XE39", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE39", "UniRef:UniRef50_C4XE39" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE39", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE39" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6935.540999999996, "isoelectric_point": 10.751730918884277 }, "id": "OAIKOHHJFB_302", "locus": "GCA000209735_00271" }, { "type": "cds", "sequence": "contig_1", "start": 150526, "stop": 150660, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTGGAGTTTCATATATTGAACTTTTAGTTGATGGTAAAACTGTAGATAAATTAAATGTTTTTAAGAAAAGAAATGCTTCATTAAGTCATAATTTAGATGGCGACCTAATTCATGTTAGAATTGATGGCTGA", "aa": "MVGVSYIELLVDGKTVDKLNVFKKRNASLSHNLDGDLIHVRIDG", "aa_hexdigest": "0cd091e3115818f9704f451dec542a25", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4880.5796, "isoelectric_point": 6.700998878479004 }, "id": "OAIKOHHJFB_303", "locus": "GCA000209735_00272" }, { "type": "cds", "sequence": "contig_1", "start": 150823, "stop": 151179, "strand": "-", "frame": 2, "gene": "rplS", "product": "50S ribosomal protein L19", "db_xrefs": [ "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02884", "RefSeq:WP_013354332.1", "SO:0001217", "UniParc:UPI0001A8C3B5", "UniRef:UniRef100_C4XE40", "UniRef:UniRef50_O31742", "UniRef:UniRef90_A5IZ68" ], "nt": "ATGAGAAATAAATTAATCGAATTAGTAGAATCAAACCAATTACGTACAGACTTCCCAGAATTCAGAGTTGGAGATAATGTTAAAGTTCACGTTCGTATTCGTGAAGGTGAAAAAGAACGTATCCAAATTTTTGAAGGTTTAGTTATTAGCAAAAAAGAATCAGGTACACGTGAAACATTTACAGTTAGAAAAATTTCTTTTGGTATAGGTGTTAATAGAACATTTCCATTACACTCACCTATGATCGCTTCAATTGAAGTTGTTCGTTCAAATAAAGTACGTCGTGCAAAACTTTACTACATGAAAAACAGAAGTGGTAAAAGTGCTCGTCTTAAGGAAATTAAACGTACAAAATAA", "aa": "MRNKLIELVESNQLRTDFPEFRVGDNVKVHVRIREGEKERIQIFEGLVISKKESGTRETFTVRKISFGIGVNRTFPLHSPMIASIEVVRSNKVRRAKLYYMKNRSGKSARLKEIKRTK", "aa_hexdigest": "1dd843e14e47f79b7c4df38568a503bd", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001A8C3B5", "ncbi_nrp_id": "WP_013354332.1", "uniref100_id": "UniRef100_C4XE40", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C3B5", "RefSeq:WP_013354332.1", "UniRef:UniRef100_C4XE40" ] }, "ips": { "uniref100_id": "UniRef100_C4XE40", "uniref90_id": "UniRef90_A5IZ68" }, "psc": { "uniref90_id": "UniRef90_A5IZ68", "gene": "rplS", "product": "50S ribosomal protein L19", "uniref50_id": "UniRef50_O31742", "kegg_orthology_id": "K02884", "go_ids": [ "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_O31742", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O31742" ], "product": "Large ribosomal subunit protein bL19" }, "genes": [ "rplS" ], "id": "OAIKOHHJFB_304", "locus": "GCA000209735_00273" }, { "type": "cds", "sequence": "contig_1", "start": 151169, "stop": 151870, "strand": "-", "frame": 1, "gene": "trmD", "product": "tRNA (guanosine(37)-N1)-methyltransferase TrmD", "db_xrefs": [ "EC:2.1.1.228", "GO:0008033", "GO:0052906", "KEGG:K00554", "RefSeq:WP_013354331.1", "SO:0001217", "UniParc:UPI0001E32F1D", "UniRef:UniRef100_C4XE41", "UniRef:UniRef50_Q98Q98", "UniRef:UniRef90_C4XE41" ], "nt": "ATGAAAATAAATTTTTTAACTCTTTTTTCAAATTACTACAAACCATTTGTAGAAGAATCAATTATTTCAAAAGCTATTGAAAAGAAATTGATTGAAATTAATGTAGTTGATTTTAGACTTTTCAGCAAAGATAAACATCACAAAGTTGATGATGAAATTTTTGGTGGAGGTCATGGAATGCTCCTGCAAATAGAACCTATTGATTTAGCACTTGATTCTCTTAAAGAAAGAGGAGGTTACAAAATTCTTGTAACTCCTCAAGGAAAGAAATTTGATCAACAAATTGCTAATGAATTAGCTAAGAAAAAAGAAATAACTTTTATTTCTGGTCGCTATGAAGGCTTTGATGAAAGAATAGTAGACCTTGTTGATGAAGAAATTTCAATTGGTGATTATGTTTTAACCGGAGGCGAATTACCTTCAATGGTAATGGCAGACAGTATCATAAGATTAGTTCCTGGCGTAATAAGAGAAGAAAGTTATGCTAATGATTCATTTCAAAATGAAGGTTTGTTAGATTATCCTCAATACACTAGACCAAGAGTGTACAAGAGCAAAGCTAAAAAAGAAGCTTTAATAGTGCCTGAAGTTTTAGTTAATGGAAATCATGCAGAAATTGAAAAATGGAAAAAAGAAGCTCAATTAAAAAAGACAAAGAAAAATCGTCCAGATATAATCGAAAGGATTAAAAATGAGAAATAA", "aa": "MKINFLTLFSNYYKPFVEESIISKAIEKKLIEINVVDFRLFSKDKHHKVDDEIFGGGHGMLLQIEPIDLALDSLKERGGYKILVTPQGKKFDQQIANELAKKKEITFISGRYEGFDERIVDLVDEEISIGDYVLTGGELPSMVMADSIIRLVPGVIREESYANDSFQNEGLLDYPQYTRPRVYKSKAKKEALIVPEVLVNGNHAEIEKWKKEAQLKKTKKNRPDIIERIKNEK", "aa_hexdigest": "cf67f528c10da360f56575dc0b732524", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32F1D", "ncbi_nrp_id": "WP_013354331.1", "uniref100_id": "UniRef100_C4XE41", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F1D", "RefSeq:WP_013354331.1", "UniRef:UniRef100_C4XE41" ] }, "ips": { "uniref100_id": "UniRef100_C4XE41", "uniref90_id": "UniRef90_C4XE41" }, "psc": { "uniref90_id": "UniRef90_C4XE41", "gene": "trmD", "product": "tRNA (guanosine(37)-N1)-methyltransferase TrmD", "ec_ids": [ "2.1.1.228" ], "uniref50_id": "UniRef50_Q98Q98", "kegg_orthology_id": "K00554", "go_ids": [ "GO:0008033", "GO:0052906" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q98", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q98" ], "product": "tRNA (guanine-N(1)-)-methyltransferase" }, "genes": [ "trmD" ], "id": "OAIKOHHJFB_305", "locus": "GCA000209735_00274" }, { "type": "cds", "sequence": "contig_1", "start": 151884, "stop": 152090, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAACGCACACAAAGAAGCTACAAAAGCACAAGAAGTTAAAAAACTTGCAGCTAAACCAGCTACAAAACCTGCTTCAAAATCAACTGCTCCTAAAAAAGTAGTTGCTGACAAAAAAGTTGCAACAAAAGCAGCAACAACTAAAACTACAAAAGCTGCTGCTAAACCTTCAGCTAAAACTGTTCGTAAAACTACAAAAAAATAG", "aa": "MNNAHKEATKAQEVKKLAAKPATKPASKSTAPKKVVADKKVATKAATTKTTKAAAKPSAKTVRKTTKK", "aa_hexdigest": "362355c0a988b88a03a798e7950e342f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7115.3544000000065, "isoelectric_point": 10.71852970123291 }, "id": "OAIKOHHJFB_306", "locus": "GCA000209735_00275" }, { "type": "cds", "sequence": "contig_1", "start": 152163, "stop": 152330, "strand": "-", "frame": 3, "gene": null, "product": "30S ribosomal protein S16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FB42" ], "nt": "ATGGTTAAAATTAGATTAAAGAGATTAGGAAGCAAATTTAATGCTTGCTACAAAATTGTGGCTGCTGACTCACACGCTCCTAGAGATGGGAAATATATTGAAGCATTAGGCCAATATAATCCACACTCAAAGGAATTTGTTTTAAATGAAGAAGCAACACAAAGATGA", "aa": "MVKIRLKRLGSKFNACYKIVAADSHAPRDGKYIEALGQYNPHSKEFVLNEEATQR", "aa_hexdigest": "78461022c437652e957a87f8934ac54f", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A432FB42", "query_cov": 0.8727272727272727, "subject_cov": 0.8727272727272727, "identity": 0.688, "score": 75.5, "evalue": 4.6e-17, "valid": false, "gene": "rpsP", "product": "30S ribosomal protein S16", "uniref50_id": "UniRef50_A0A432FB42", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_A0A432FB42", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FB42" ], "product": "30S ribosomal protein S16" }, "genes": [], "id": "OAIKOHHJFB_307", "locus": "GCA000209735_00276" }, { "type": "cds", "sequence": "contig_1", "start": 152570, "stop": 152842, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01418.23" ], "nt": "ATGAAAAATAATGACAAAAGAATAATAAGCCGTCCATTAATGGATGATACAGTTGAATTAACTAAAAGTGAAAAAAGAATTCGAGATTTTATTAATAATTTTGAAGGTAATTATTTTAACTTTTCACAAAATAAGTTAGCTAAAAATACTGAATCAAGTGATGCGGGAGTTTCTAGATTTATTCGTAAATATGGTTTTAAAGACTATCGCACTTTTATTGCTTCAATAAATAACAAATTAACAGAATTTGAAAAAACTTATCCTACTATTTGA", "aa": "MKNNDKRIISRPLMDDTVELTKSEKRIRDFINNFEGNYFNFSQNKLAKNTESSDAGVSRFIRKYGFKDYRTFIASINNKLTEFEKTYPTI", "aa_hexdigest": "82241fe3d94061dd2cd7df428f06b7f5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01418.23", "name": "HTH_6", "length": 60, "aa_cov": 0.6666666666666666, "hmm_cov": 1.0, "evalue": 2.211448264060991e-11, "score": 39.7202033996582, "start": 18, "stop": 77 } ], "seq_stats": { "molecular_weight": 10671.916599999988, "isoelectric_point": 9.598262596130368 }, "id": "OAIKOHHJFB_308", "locus": "GCA000209735_00277" }, { "type": "cds", "sequence": "contig_1", "start": 153014, "stop": 153406, "strand": "+", "frame": 2, "gene": "rpiR", "product": "RpiR family transcriptional regulator", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE43", "UniRef:UniRef90_C4XE43" ], "nt": "ATGAATTTAGTTTCTAATTTATTAAAAATTGGTAAACCTGTTATAGCTCATAGTGACTTTCATATTTTCTTTCCATCCTTAGCTCATGTTACTGAAAATGATGTTGTAATTGTTTACTCAAATAATTTACAAACAATGGAAGCTCACTTTGTTATCGAGCAAGCTAAAAAACAAAAAGCGCCAATTATTGTTTTAACTTCTAGCCAAGAAGAAGATAAATTAATTGCAGTTAAATTAAGATATCACAAAATTCAAAGTTCAACTATGTTAGTTCCTTCAAGTTCAAAAATAGCTCAAATGTTAATTACCGATTTATTATTTGAAGCTGTTTTAGAGCATAATAAGGAAAATACAAGTAAACTTAAAAAAGCAAGAATACTTATTAAAAATTGA", "aa": "MNLVSNLLKIGKPVIAHSDFHIFFPSLAHVTENDVVIVYSNNLQTMEAHFVIEQAKKQKAPIIVLTSSQEEDKLIAVKLRYHKIQSSTMLVPSSSKIAQMLITDLLFEAVLEHNKENTSKLKKARILIKN", "aa_hexdigest": "dcf30baf8efb0cff998da307d29276ff", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpiR" ], "truncated": "both", "pseudogene": { "start": 152714, "stop": 153436, "observations": { "mutation": [ 152840, 153404 ], "stop": [ 152840, 153404 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE43", "query_cov": 1.0, "subject_cov": 0.4377104377104377, "identity": 1.0, "score": 246.0, "evalue": 4.33e-80, "gene_start": 1, "gene_end": 130, "reference_start": 158, "reference_end": 287, "reference_sequence": "MKKFNIITIMKNNDKRIISRPLMDDTVELTKSEKRIRDFINNFEGNYFNFSQNKLAKNTESSDAGVSRFIRKYGFKDYRTFIASINNKLTEFEKTYPTIWNDNEVDNDYKFIAASHRYAIDNVYDEDLLKDINKAAEIINKSKNIYIHGCGSSQRISMNLVSNLLKIGKPVIAHSDFHIFFPSLAHVTENDVVIVYSNNLQTMEAHFVIEQAKKQKAPIIVLTSSQEEDKLIAVKLRYHKIQSSTMLVPSSSKIAQMLITDLLFEAVLEHNKENTSKLKKARILIKNWSLIDKAHSE" }, "paralog": false, "identity": 0.9916666666666667, "score": 462.0, "evalue": 1.33e-167, "description": "Internal stop codon at 152840, 153404. Nonsense mutation around 152840, 153404.", "psc": { "uniref90_id": "UniRef90_C4XE43", "product": "RpiR family transcriptional regulator", "uniref50_id": "UniRef50_C4XE43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE43", "UniRef:UniRef50_C4XE43" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE43" ], "product": "RpiR family transcriptional regulator" } }, "id": "OAIKOHHJFB_309", "locus": "GCA000209735_00278" }, { "type": "cds", "sequence": "contig_1", "start": 153447, "stop": 153692, "strand": "+", "frame": 3, "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "db_xrefs": [ "GO:0006364", "KEGG:K02834", "SO:0001217", "UniRef:UniRef50_A0A3P8L789", "UniRef:UniRef90_A0A3P8L789" ], "nt": "ATGCCTAAAAGTATAAACACATTAAGAAGAGAATCACAAATTAGAACATTATTAGCAGAAATAATAACAAATGATTTAACAAATACAAATATTATTAATCCTACATTGGTTGATTGCGAATTATCAGGTGATTTATCACATGTAAAAATCTATATGTCATTTAGCGAAAATGAAAAGAGAGGATTAGAAGCTTTACAAAATGCTTCTGGATACATTAGAACAATTTTGGCTAAAAGTTTAAATTGA", "aa": "MPKSINTLRRESQIRTLLAEIITNDLTNTNIINPTLVDCELSGDLSHVKIYMSFSENEKRGLEALQNASGYIRTILAKSLN", "aa_hexdigest": "5888db5fd4d08b4f705c51a66dd6f053", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "rbfA" ], "truncated": "3-prime", "pseudogene": { "start": 153447, "stop": 153845, "observations": { "mutation": [ 153690 ], "stop": [ 153690 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8L789", "query_cov": 1.0, "subject_cov": 0.5664335664335665, "identity": 0.889, "score": 142.0, "evalue": 5.04e-42, "gene_start": 1, "gene_end": 81, "reference_start": 1, "reference_end": 81, "reference_sequence": "MAKNINVLRRESQIRTLLAQIITNDLTNTNIINPTLVDCELSADLSHVKVYMSFSENEKRGLEALQNASGYIRSSLSKSLNWRKVPELHFYIDEVSKKGLSIDKILYNIRDKENFETNNKVSFEQKETNYGNDVNPKTCTKKL" }, "paralog": false, "identity": 0.8181818181818182, "score": 219.0, "evalue": 1.13e-74, "description": "Internal stop codon at 153690. Nonsense mutation around 153690.", "psc": { "uniref90_id": "UniRef90_A0A3P8L789", "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "uniref50_id": "UniRef50_A0A3P8L789", "kegg_orthology_id": "K02834", "go_ids": [ "GO:0006364" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8L789", "UniRef:UniRef50_A0A3P8L789", "KEGG:K02834", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8L789", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8L789" ], "product": "Ribosome-binding factor A" } }, "id": "OAIKOHHJFB_310", "locus": "GCA000209735_00279" }, { "type": "cds", "sequence": "contig_1", "start": 153885, "stop": 154424, "strand": "+", "frame": 3, "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "db_xrefs": [ "GO:0006364", "KEGG:K02834", "SO:0001217", "UniRef:UniRef50_A0A3P8LI57", "UniRef:UniRef50_C4XE44", "UniRef:UniRef90_C4XE44" ], "nt": "TTGCATACCTTAAATAAAAAGAATGAATTAATGAAAATAAGTTATTTAAAAAATGAACAAAATGAAAAATCATCTAAAGCTTGGTATGTTTTAACAACAAACAATTCATCTTATGGTCTTTTTGGCTTAAAAAACTATAAAACAGATGTTTTGGAAAAAAATTGTTTTAAAAAAATAAATTTAAAAAATAGATCTTTTATTAATTTTAATAATGACTTTGTACACTCTGCTTATACTTCAGACGATGAATTAAGATTATTAGAAATATCAGTTAATACTAAAAAAACTGAAGTAAAAAATTTTAAAGATTTAAATAAAATTAACAATGAAAATTCATCTACATCAATGAATAGATATTATAAGGAAGATTGTATTGTTTTTTACAATAAAAAAACTAGAATTACATTATTTAATGATAATAAAGAATATATCGTAAAAAGAAAATGTTTAGTTTTTGATTGCATCAGTGAAACGCTTTATAGAGCAAATAAAGGCGATGAACTAAACTTTCATAAAATGTTAATAATTGAAAAAAGATAA", "aa": "MHTLNKKNELMKISYLKNEQNEKSSKAWYVLTTNNSSYGLFGLKNYKTDVLEKNCFKKINLKNRSFINFNNDFVHSAYTSDDELRLLEISVNTKKTEVKNFKDLNKINNENSSTSMNRYYKEDCIVFYNKKTRITLFNDNKEYIVKRKCLVFDCISETLYRANKGDELNFHKMLIIEKR", "aa_hexdigest": "b80e09c667bb6c244e28f358f999ec67", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3P8LI57", "query_cov": 0.9497206703910615, "subject_cov": 1.0059171597633136, "identity": 0.7, "score": 234.0, "evalue": 1.72e-76, "valid": false, "uniref50_id": "UniRef50_A0A3P8LI57" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LI57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LI57" ] }, "genes": [ "rbfA" ], "truncated": "5-prime", "pseudogene": { "start": 153585, "stop": 154424, "observations": { "mutation": [ 153690 ], "stop": [ 153690 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE44", "query_cov": 1.0, "subject_cov": 0.5343283582089552, "identity": 0.9940000000000001, "score": 352.0, "evalue": 1.31e-120, "gene_start": 1, "gene_end": 179, "reference_start": 157, "reference_end": 335, "reference_sequence": "MKLIANKGDIMPKSINTLRRESQIRTLLAEIITNDLTNTNIINPTLVDCELSGDLSHVKIYMSFSENEKRGLEALQNASGYIRTILAKSLNWRKVPELHFYIDEVSKKCLSIDRILYDIKDEKNFEANNKISYNEHLTNFGNSKIFELDKYEPQIVLHTLNKKNELMKISYLKNEQNEKSSKAWYVLTTNNSSYGLFGLKNYKTDVLEKNCFKKINLKNRSFINFNNDFVHSAYTSDDELRLLEISVNTKKTEVKNFKDLNKINNENSSTSMNRYYKEDCIVFYNKKTRITLFNDNKEYIVKRKCLVFDCISETLYRANKGDELNFHKMLIIEKR" }, "paralog": false, "identity": 0.996415770609319, "score": 561.0, "evalue": 1.9e-205, "description": "Internal stop codon at 153690. Nonsense mutation around 153690.", "psc": { "uniref90_id": "UniRef90_C4XE44", "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "uniref50_id": "UniRef50_C4XE44", "kegg_orthology_id": "K02834", "go_ids": [ "GO:0006364" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE44", "UniRef:UniRef50_C4XE44", "KEGG:K02834", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE44", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE44" ], "product": "Ribosome-binding factor A" } }, "id": "OAIKOHHJFB_311", "locus": "GCA000209735_00280" }, { "type": "cds", "sequence": "contig_1", "start": 154986, "stop": 155312, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAACTTAAACCTTTCAGCGAAAATGAATTAAAAGTGTTATTTGAAAAATTTAATGACTATATTATTGTTGGAGGAATGCCAGAGATTGTTAAAAATTTCATAAAGCAAAAAAATTTTTCAAATATTTTAAATCAACAAAAAGAATTACTTGAAGTTTATAAGCAAGACATTGGTAAATATGCAGAAAGTTTGGATAGATACAAAATTTTAAAAGTTTATGAAAGTATTTCTCCATTTTTAGGTAAAGAAAACAAAAGATTTATGTTTTCAAAAGTTGAACATAATGCAAGGTTTAGAGATTATTTTTCAACAATGGAATGA", "aa": "MKELKPFSENELKVLFEKFNDYIIVGGMPEIVKNFIKQKNFSNILNQQKELLEVYKQDIGKYAESLDRYKILKVYESISPFLGKENKRFMFSKVEHNARFRDYFSTME", "aa_hexdigest": "dacaee29711aa2e76541ce43d720af53", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13003.91509999999, "isoelectric_point": 9.023849296569825 }, "id": "OAIKOHHJFB_312", "locus": "GCA000209735_00281" }, { "type": "cds", "sequence": "contig_1", "start": 155949, "stop": 156179, "strand": "-", "frame": 3, "gene": null, "product": "Sigma-70, region 4", "db_xrefs": [ "KEGG:K09787", "RefSeq:WP_013354325.1", "SO:0001217", "UniParc:UPI0001A8C3BB", "UniRef:UniRef100_C4XE46", "UniRef:UniRef50_C4XE46", "UniRef:UniRef90_C4XE46" ], "nt": "ATGAATAAAGATATTAAAGATATAGAAAATAGAGAGCACTTATTAGAACTATTTGATAAATTTAATTCATTTTTAACTCAAACACAAAAACAAGCTTTTCAATTATATTTCATTGAAGATCTTTCTTATTCTGAAGTTGCCAACATTACAGCAACCACCCGTTCTGGAGCTTATGATAGTGTGAATAAAGCAATTAAAAAATTACTAGATATTGAAGCAAAAATGCAATAA", "aa": "MNKDIKDIENREHLLELFDKFNSFLTQTQKQAFQLYFIEDLSYSEVANITATTRSGAYDSVNKAIKKLLDIEAKMQ", "aa_hexdigest": "ca972cf6120e9f43c803240e82563c66", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI0001A8C3BB", "ncbi_nrp_id": "WP_013354325.1", "uniref100_id": "UniRef100_C4XE46", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C3BB", "RefSeq:WP_013354325.1", "UniRef:UniRef100_C4XE46" ] }, "ips": { "uniref100_id": "UniRef100_C4XE46", "uniref90_id": "UniRef90_C4XE46" }, "psc": { "uniref90_id": "UniRef90_C4XE46", "product": "Sigma-70, region 4", "uniref50_id": "UniRef50_C4XE46", "kegg_orthology_id": "K09787" }, "pscc": { "uniref50_id": "UniRef50_C4XE46", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE46" ], "product": "Sigma-70, region 4" }, "genes": [], "id": "OAIKOHHJFB_313", "locus": "GCA000209735_00282" }, { "type": "cds", "sequence": "contig_1", "start": 156172, "stop": 156495, "strand": "-", "frame": 2, "gene": "ftsY", "product": "Cell division protein ftsY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_E4QS11" ], "nt": "ATGAAAGAACTTGAAAAAATGTATGGCATTATTGCTAGATTTCAAGATAAAGCGCCACATGAATGCTTATTAGTTTTAGATGCAACAACTGGTCAAAATGGAGTTAGCCAAGCTAGAAGTTTTAAAGAAGTTGCGGCTCCAACTGGAATTATATTAACTAAAATGGATGGAACTTCTAAAGGTGGAATTGTACTTTTAATTAAAGACGAATTTGATTTGAATGTTAAATATATTGGACTTGGCGAAGGGCTAGATGATCTTCAAGAATTTGATTTAGATAACTTCATTTATGAAATGACAAAGGACTTAATTGTAAATGAATAA", "aa": "MKELEKMYGIIARFQDKAPHECLLVLDATTGQNGVSQARSFKEVAAPTGIILTKMDGTSKGGIVLLIKDEFDLNVKYIGLGEGLDDLQEFDLDNFIYEMTKDLIVNE", "aa_hexdigest": "994f238c5003b8b035c44361ee952128", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PPJ9", "query_cov": 0.9719626168224299, "subject_cov": 0.9203539823008849, "identity": 0.75, "score": 155.0, "evalue": 2.7e-47, "valid": false, "product": "Signal recognition particle-docking protein FtsY", "uniref50_id": "UniRef50_E4QS11" }, "pscc": { "uniref50_id": "UniRef50_E4QS11", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_E4QS11" ], "product": "Cell division protein ftsY" }, "genes": [ "ftsY" ], "id": "OAIKOHHJFB_314", "locus": "GCA000209735_00283" }, { "type": "cds", "sequence": "contig_1", "start": 156649, "stop": 156978, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00448.28" ], "nt": "ATGAGTGATATTAATGCTAAATTAGTTTATGCAATTATCACACACATTAAAAACGAAGTTAGAGCTAGAGGTTTAAAAGATACTAAAGATATAGGTGAATTAGTTGCTGATCAAATGTTTGTTGTTTATACAAACAAAAGTATTGTTGATACAACTTTAAATTATCAAGACGGGAGATTAAATATCTTTATTTTTGTTGGAGTTAATGGTAGTGGTAAAACAACTTCAATTGCTAAAATAGCCTATAAATATATAAAAGAAGGAAAAAAAGTTTTAATTGCTGCAGGCGATACTTTTAGAGCTGGTGCAGTAGATCAATTAGGTGTGTGA", "aa": "MSDINAKLVYAIITHIKNEVRARGLKDTKDIGELVADQMFVVYTNKSIVDTTLNYQDGRLNIFIFVGVNGSGKTTSIAKIAYKYIKEGKKVLIAAGDTFRAGAVDQLGV", "aa_hexdigest": "54fa3717e7fe33575a41645ca6c556b8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00448.28", "name": "SRP54", "length": 48, "aa_cov": 0.44036697247706424, "hmm_cov": 1.0, "evalue": 7.812625091097789e-20, "score": 67.41764831542969, "start": 61, "stop": 108 } ], "seq_stats": { "molecular_weight": 11912.65809999999, "isoelectric_point": 9.362372779846194 }, "id": "OAIKOHHJFB_315", "locus": "GCA000209735_00284" }, { "type": "cds", "sequence": "contig_1", "start": 157326, "stop": 157529, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021806470", "UniRef:UniRef90_UPI0021806470" ], "nt": "TTGAGATATTTACTTAGTAATCAATTAGGGTATAAAGAAGATTTAGTTGCATATGATAATAAAGGTGTAGTAGAAATAATTAACAATGACATTTTCAATGTGTTTGTATTGAAATTTGAAGGCAATGAGTTTTCATTCCTGAGTTATAATGATGCAATTTCATTTTTAAAAGATTACATAATAACAAGATCAAAATTAATATAA", "aa": "MRYLLSNQLGYKEDLVAYDNKGVVEIINNDIFNVFVLKFEGNEFSFLSYNDAISFLKDYIITRSKLI", "aa_hexdigest": "e1482f762bc20ed36c7d2630a9d9637d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021806470", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.985, "score": 131.0, "evalue": 7.65e-39, "valid": true, "uniref50_id": "UniRef50_UPI0021806470" }, "pscc": { "uniref50_id": "UniRef50_UPI0021806470", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021806470" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7862.912499999995, "isoelectric_point": 4.810363578796387 }, "id": "OAIKOHHJFB_316", "locus": "GCA000209735_00285" }, { "type": "cds", "sequence": "contig_1", "start": 157614, "stop": 157955, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTTTCAAAGAAAAACATGTATAGAATTTTTAAACAAAATAATTTCTTCTAAATCATTTCCAAGTGCTTCTGAACCCGAAGACAAACAAAAACTTTTAAAAGAAATTATTGAAGAACATAAGAATAAAAACAAATTAAATAATAGCTTTTTTGAAAATAATTTGCCCTTACTTATTAATGATAAAAACATTGAATTATTTTCTTTCTATTCAAATAATAATGTTGACATCTTATCAAAAAGATATTCAATACCTTCTAACATACTACTTGAGTTTCCAACTTTTTATTCTAAAGATGATTATGAAAATAACATAGCTCTTTATTTTGACGAAAATTGA", "aa": "MIFQRKTCIEFLNKIISSKSFPSASEPEDKQKLLKEIIEEHKNKNKLNNSFFENNLPLLINDKNIELFSFYSNNNVDILSKRYSIPSNILLEFPTFYSKDDYENNIALYFDEN", "aa_hexdigest": "847c1d7cacac38fcc8b6662a8cebaf44", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13409.995499999979, "isoelectric_point": 5.182545280456542 }, "id": "OAIKOHHJFB_317", "locus": "GCA000209735_00286" }, { "type": "cds", "sequence": "contig_1", "start": 158847, "stop": 159635, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTAAAAAATTATTTTTATCTTTAATTCCAACAGTTGTTTTGATTCCATCATGCAATAATTCAGCCTCTATGAATTATTATATGCAAAACAATAATTTAAAAAACGCAAGCAAATATGAAATTAATTTAGAAGGACAAAAATACCAATATACTGATATTGAATCATTTATGTCTAATTTAATAAATAGTAATAAAATAAAAGTTGAAAACAAATTAGTAATAGGCAAAAAGAAAAACAATAGTTCAAATAAAATTCTAGATTCTACTATTTTTTATAATTTCGACCCAAATAAACTTAAAAAAGTTTATCAAAATAAATTCCAAAAAACGGTTTATTCAGTTGATGAAGTAATAGAATCGTTTAGAGAAGAAAGCTCATATATTAAAAAATATCTTTTTGCCAATAAAGAATTTGATTCAGAAGAAAAAGTAAAACAGTATATAAAAACTCAACTTAAAAACGGTTTTAATAAATATGTTTATTATTCTAATAATGGGAAAAAATATAATGCTATGAATTGGCAAGATTCATTAGAATTGCTAAAAGATAAATATAATAAAGATAACAATGTGTTTTATCCTATTTTTGATAACAACAATAATGTAATTTATAATAATTTACTTAATTATGAAACAATGAATTCTATTAGTAAAAAAATTCTTGAAAAGTTAATGAAGTTTAAAGTAGAAATAAAATTTGGTTTAGATCTCAATGATGAAAAGTCTTATGACTACAATGAAAAGGATTTAGGTTTTAAAGTAAATGTAAAAAATAATAAATGA", "aa": "MKIKKLFLSLIPTVVLIPSCNNSASMNYYMQNNNLKNASKYEINLEGQKYQYTDIESFMSNLINSNKIKVENKLVIGKKKNNSSNKILDSTIFYNFDPNKLKKVYQNKFQKTVYSVDEVIESFREESSYIKKYLFANKEFDSEEKVKQYIKTQLKNGFNKYVYYSNNGKKYNAMNWQDSLELLKDKYNKDNNVFYPIFDNNNNVIYNNLLNYETMNSISKKILEKLMKFKVEIKFGLDLNDEKSYDYNEKDLGFKVNVKNNK", "aa_hexdigest": "466aad8a694c451aa6689ecf538d868b", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 31110.143100000023, "isoelectric_point": 9.340324592590331 }, "id": "OAIKOHHJFB_318", "locus": "GCA000209735_00287" }, { "type": "cds", "sequence": "contig_1", "start": 159664, "stop": 160170, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATCCTATTTATTATTCAAAAAAACCAATAGAAACAGAAGATGGTTACACCGACAAGCTATTTCCTATTGATCAATTTGATCCAACAAAAAAAGCTTTTGAAGACAAAAAAATACTAGCAAATATTTATAATGAAGATCCATTAAAAAATTGCTTTTTATATTCAGAAATAGGAAACTTTAATATTCAAAGCGATTTGTTAAAAAATAATAGAAACAAATTTATTCCATTTTTAAATTCATTGAATATAAGAACAAAAGAAGTTTCAAATATATCATTTTTTAATAATTCAAATTATCAAGATATGGAATTGTTGCAAACAAAAACACTTTATTATTTTGATTATAAAAACAATAAATACTTCTTTACAAATTTAGACAAAGCGAGAAATTATTTTATCAATGTTATTATTAACCCAAAAATGGAATTATATAATTTAACCAAAAGAACCAAAATTATATTTATGAATAAAGAGTTCTGTACATTGAATGAAGCAGTATCTTGA", "aa": "MNPIYYSKKPIETEDGYTDKLFPIDQFDPTKKAFEDKKILANIYNEDPLKNCFLYSEIGNFNIQSDLLKNNRNKFIPFLNSLNIRTKEVSNISFFNNSNYQDMELLQTKTLYYFDYKNNKYFFTNLDKARNYFINVIINPKMELYNLTKRTKIIFMNKEFCTLNEAVS", "aa_hexdigest": "7e129a86dc9ae178808e7d802a2d0e76", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20145.779200000008, "isoelectric_point": 8.669337654113772 }, "id": "OAIKOHHJFB_319", "locus": "GCA000209735_00288" }, { "type": "cds", "sequence": "contig_1", "start": 160411, "stop": 160587, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACGGTTATACTGAAAGTTTTTATTACTTCAATGGTAAAAAATATAACTTTGTTGATATAGATTTATTAAAAAGAGATGTTGTAAATTACTATTTAAAAGGAAACAACAAAATTATAAACAACTTATTAGAAAAAGATGAAGCTGAAAAGAACATAAAAATTATTTACTCATGA", "aa": "MDGYTESFYYFNGKKYNFVDIDLLKRDVVNYYLKGNNKIINNLLEKDEAEKNIKIIYS", "aa_hexdigest": "5f30dddf43e072cd30bfbcd985bdd02e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6978.839899999999, "isoelectric_point": 6.139145469665528 }, "id": "OAIKOHHJFB_320", "locus": "GCA000209735_00289" }, { "type": "cds", "sequence": "contig_1", "start": 160642, "stop": 161013, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGATTTGTTGCCAGTAGAAAAAAAATCATTTGCTAAATATTTATTATATGCTTTCATAGAAATAGATAATATAGTTAAAAAAGGTAGTTGGTCAAGCAAAGTTAATCTTTTAGTAGAAACTACAAAATATATTCATTCAATAATAGATGCAAAAGCAGCAGCATTAGAAATAGCTTTAAATGTTGTCGGAGGTCCAGTTTCTGCTTTTGGTGGTTTATTGTCTAACTTTTATGTTAAATTTTACAAAGCATTATTTGGTGAATTTAGCCAATTTAACTATAAATACGTTGTCAATGCAGGAGATAAAGGTAATGTCGATTATATATGGAATGGTGGATTGCGTTATGTTGGTTTATTTCATTGA", "aa": "MKKDLLPVEKKSFAKYLLYAFIEIDNIVKKGSWSSKVNLLVETTKYIHSIIDAKAAALEIALNVVGGPVSAFGGLLSNFYVKFYKALFGEFSQFNYKYVVNAGDKGNVDYIWNGGLRYVGLFH", "aa_hexdigest": "c9ad9b5a9f148db23e3d8e95c12b079b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13798.869099999989, "isoelectric_point": 9.323756217956543 }, "id": "OAIKOHHJFB_321", "locus": "GCA000209735_00290" }, { "type": "cds", "sequence": "contig_1", "start": 161110, "stop": 161208, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCGACAGTAAAAAACAACTTTGATAATGTGAATGAATTAGTAAAAGATATTCTTCTAAATAATGAAGATTTATTAAACAAAGATAAAAGTTTTTGA", "aa": "MSTVKNNFDNVNELVKDILLNNEDLLNKDKSF", "aa_hexdigest": "16e0d69176099dc4f8a134ae5fe80175", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3725.1410000000005, "isoelectric_point": 4.528214836120605 }, "id": "OAIKOHHJFB_322", "locus": "GCA000209735_00291" }, { "type": "cds", "sequence": "contig_1", "start": 161224, "stop": 161487, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGCTAAATTATTGAAATATAAAATAGCATGCAATTTTTTGGATGTAATTGCAAGTGGCATATTTAATGCAGTATATAGATTTTCAAGTGAAGATTATGATCCAGAATCTCTCATTTTTCCTGAATATGCAATATATGATAATTATGAACATTATTTTGAAATTTATAGTTTAAAGCGATTTAAAAAACCAGTTTTTTCAAAAAATTATGACAAATTTACTAAACAATCATATGATACTAACAACAGCTTACAAGAATGA", "aa": "MIAKLLKYKIACNFLDVIASGIFNAVYRFSSEDYDPESLIFPEYAIYDNYEHYFEIYSLKRFKKPVFSKNYDKFTKQSYDTNNSLQE", "aa_hexdigest": "58bb8d6021ab725a8297f91dfee73712", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10441.674799999988, "isoelectric_point": 5.790495109558106 }, "id": "OAIKOHHJFB_323", "locus": "GCA000209735_00292" }, { "type": "cds", "sequence": "contig_1", "start": 161533, "stop": 161970, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAGTATTGTCACATCACTTTTCAAAATTGTGTATTTTAATGAAAACATAAAGAAAAATGTTGAAATTACACCATTTACACCTATAACAATAAATGTTGAAAAAGATCATTTAAACAATTTCAAAAAATGTACCAAAAACTTTTGTTTTGAAGGCACTAACCTATACGATTATTTATATAACAAATATGATCTTTTAGCAAAAGGAAAGAATCTAATTTTCTTTCTAGTTTTATATATTATATGTTGCGGGTTTAACAAAATTATAGAATATAAAGCAAATCAAAAAGTGGTTGATGAATATAAAACATATTTTAAAATGATCATAAATGACATTAGCAAAATTTTTGCATCATTATTACCAAAATATTTCATTCATGAAGGAATTAGTGAATCAGAAAACTCAGATAATCAAGTAAATATATCAAAAATTTGA", "aa": "MNSIVTSLFKIVYFNENIKKNVEITPFTPITINVEKDHLNNFKKCTKNFCFEGTNLYDYLYNKYDLLAKGKNLIFFLVLYIICCGFNKIIEYKANQKVVDEYKTYFKMIINDISKIFASLLPKYFIHEGISESENSDNQVNISKI", "aa_hexdigest": "ecc91630ab01a4ebb295dfa50394d25b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17045.63569999999, "isoelectric_point": 8.55664691925049 }, "id": "OAIKOHHJFB_324", "locus": "GCA000209735_00293" }, { "type": "cds", "sequence": "contig_1", "start": 162028, "stop": 162612, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCGATATTTTTAGGACGAAACGGGAAAGTTTTTACTTCATTAGAAAAAGCAAGAGATTCATATTTTAATATAAATCAAGTTTACAAATTAGGCAATATTTATGCAAACAGTAAAGAGGAACTTTACAATTTCATTTTAAATAAATCAGTAAATAATAAAGATTTTTTGATAGAAGTATTAAATGAAATAAAAAGCAAATTGAATTTTAATTCACCTAACGCAACTATTACAGAATTTTTCAAAAGTCCAAGCGGACAAATTACAAGTTTTAAAAAAGAAGACCTTAAAGATGCAGACAAAAAAAGCAGAATTGAAGATTACATTAAAAATGTTGCAAAGAAATATGTAATCTATTATGATGAAGAAAATAATCCTCACTTAAAAGCGGTTGAAGATTATATATTTGATAGAATATCAAACTCTGAATTTATTAAGAGTAAAATCACTAGAATTAATTCTAATAAAGGATATAACAAATATTATGTAGACAATTCCAAAGATGATGAATTTACTTTGTATGGGCCAAGAATTTTAGAGACTCCTTTTGAAAATATTGAAGAAATAACTGATACAACTAAATGA", "aa": "MPIFLGRNGKVFTSLEKARDSYFNINQVYKLGNIYANSKEELYNFILNKSVNNKDFLIEVLNEIKSKLNFNSPNATITEFFKSPSGQITSFKKEDLKDADKKSRIEDYIKNVAKKYVIYYDEENNPHLKAVEDYIFDRISNSEFIKSKITRINSNKGYNKYYVDNSKDDEFTLYGPRILETPFENIEEITDTTK", "aa_hexdigest": "2054cc1f1f9055abe870cfdebc8b67bb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22761.299300000013, "isoelectric_point": 6.642227363586426 }, "id": "OAIKOHHJFB_325", "locus": "GCA000209735_00294" }, { "type": "cds", "sequence": "contig_1", "start": 162658, "stop": 162945, "strand": "-", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "RefSeq:WP_015510734.1", "SO:0001217", "UniParc:UPI0001A8C3BF", "UniRef:UniRef100_C4XE50", "UniRef:UniRef50_C4XE50", "UniRef:UniRef90_C4XE50" ], "nt": "ATGATGGGGGCATCATTTGTTGTCGCATCATCTTGTGGTTCTAAAGAACAAGAGCAAGAAAAAGTTTTAAGTGATAATGAATATGTTGAGAAACACTCTATAACAAGAGACACAAATATTAAGGAAACAAGATATATTAGTCAAAATGTAAACAAAGTTTTTGACAACCACATACAATTAGAAAATTTTGTTAAAAATAATATAAAATCTGTTGATTTGAAATTTTTAAAAAACTCAAAAAAATTTGATGATATAAATAAAAATAATCCACAATTTATGTCCAATTAA", "aa": "MMGASFVVASSCGSKEQEQEKVLSDNEYVEKHSITRDTNIKETRYISQNVNKVFDNHIQLENFVKNNIKSVDLKFLKNSKKFDDINKNNPQFMSN", "aa_hexdigest": "7662c19fd6cefd5fd407ba5ab9e325aa", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C3BF", "ncbi_nrp_id": "WP_015510734.1", "uniref100_id": "UniRef100_C4XE50", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C3BF", "RefSeq:WP_015510734.1", "UniRef:UniRef100_C4XE50" ] }, "ips": { "uniref100_id": "UniRef100_C4XE50", "uniref90_id": "UniRef90_C4XE50" }, "psc": { "uniref90_id": "UniRef90_C4XE50", "product": "Lipoprotein", "uniref50_id": "UniRef50_C4XE50" }, "pscc": { "uniref50_id": "UniRef50_C4XE50", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE50" ], "product": "Lipoprotein" }, "genes": [], "id": "OAIKOHHJFB_326", "locus": "GCA000209735_00295" }, { "type": "cds", "sequence": "contig_1", "start": 162982, "stop": 163221, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCATTATTAAACAAACAATTGATAAAATGAATGAACTTGTATCTAATTTAGGAAATTATCAAGCGTTTAAATTTGAAGAAAACGTAAATCCCAAAAACTTTTATGACAATACAAAAATAATAAAACTAAATAATTTGACATATGACCTGATAAACTTATTTTTAATAACATTTGTTATTTTAACACTCAGCATTTTGCCAATAATATTTACTTTTAAAAGGAGAAAACATGAATAG", "aa": "MFIIKQTIDKMNELVSNLGNYQAFKFEENVNPKNFYDNTKIIKLNNLTYDLINLFLITFVILTLSILPIIFTFKRRKHE", "aa_hexdigest": "093021895d27c9b3ac1f070b46c997bc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9458.04919999999, "isoelectric_point": 9.346964836120605 }, "id": "OAIKOHHJFB_327", "locus": "GCA000209735_00296" }, { "type": "cds", "sequence": "contig_1", "start": 163252, "stop": 163563, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCAAAATTAATTTCTTTGATTTTTTAAATCATTTATACAGATCAAATCAAACAAAATTTAACACCACAATTAACATGGCTGAATTTTATATTGTTAAAGATAATAATTTAATAAAATACTTAGATTCAAATAGTAAAATAACTGATTCAGATTTAAATTTTATTAGCGAAAAAATGGTGAATTTTGTTGGTGATAAAGTTATTATTGATTTTAATAAATATGCACAAAGTGAACTTGAAAATTCAATAACAACAACAATTACTAAATTCAATTTATATTTTCCTGAAATAGATTCAAAAGAAAAATGA", "aa": "MFKINFFDFLNHLYRSNQTKFNTTINMAEFYIVKDNNLIKYLDSNSKITDSDLNFISEKMVNFVGDKVIIDFNKYAQSELENSITTTITKFNLYFPEIDSKEK", "aa_hexdigest": "fa56cdd38d71c654c2de82f713b72066", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12219.709499999986, "isoelectric_point": 5.297644233703613 }, "id": "OAIKOHHJFB_328", "locus": "GCA000209735_00297" }, { "type": "cds", "sequence": "contig_1", "start": 163705, "stop": 164532, "strand": "-", "frame": 2, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE51", "UniRef:UniRef90_C4XE51" ], "nt": "ATGATTTTTCTATTGGATTTTATTATAGTTTGTGTGTCAACATTATTTATTCTGCATTTAACATTTGTATCAAACCAAAAATCGATATATGAAAACACATTAAATTGTAGATATTTTAATAAACCTAAAATTGTTTTAGCTTGGTCAATATATTCATTTATTTTATTATCCGCATTTTCATTATTAAAGTCTTTTGCAATAAGTTCATTGCATTTATATAGAGACACGAATATAAAAAGTTTTCTACTTTATTTTATAGGCTATTTTCTAAGTAATTTATTGATAATCATTTTTATTATTTTAGTTTTTGCAAGTTTTATATTTATAACAAAATCTTATAAAAAGTCTCTCGTCATTGCAACTTGTATATTACCTTTTTTTACTGTTGTGTCTTTATCATCAAAAACAATAGAAGCGCGAGCAAATAAAAAGGTAGAAATAAACAAGGATATAATAGTTTCTGGTGACAAGTATTACTTTTTATCAGATGATGAGATTAAAAGTAAATATAATTTGAACTTAATTTCATCTATATATTTTTTACCCAAAAATACTATAAATAAGTACTTTGATGAGAATTTCTTGACTTATAAAAATAATTCAGATACAAAAAATTATATATTCTCAACATTCAATTTGTCTATTCTAAAAAATAATAAAAAATTTGACAAAGATAAATTTAGAATTTATCATAACGATGGTGAGTTTTTTATAAAAATGAGTACAAATGAATTGGCTAATTATTTATATAGCAAATTAAAAAAATATTTTGAATCAGGTGGAGTTAATTTAGATAAATTACTTAAAAAAATAAAATCTAATATATGA", "aa": "MIFLLDFIIVCVSTLFILHLTFVSNQKSIYENTLNCRYFNKPKIVLAWSIYSFILLSAFSLLKSFAISSLHLYRDTNIKSFLLYFIGYFLSNLLIIIFIILVFASFIFITKSYKKSLVIATCILPFFTVVSLSSKTIEARANKKVEINKDIIVSGDKYYFLSDDEIKSKYNLNLISSIYFLPKNTINKYFDENFLTYKNNSDTKNYIFSTFNLSILKNNKKFDKDKFRIYHNDGEFFIKMSTNELANYLYSKLKKYFESGGVNLDKLLKKIKSNI", "aa_hexdigest": "d0db43746aa6a7a40d47c6510953ecc0", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 163402, "stop": 164679, "observations": { "mutation": [ 163599, 163614, 163707, 164535, 164628 ], "stop": [ 163599, 163614, 163707, 164535, 164628 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE51", "query_cov": 1.0, "subject_cov": 0.48672566371681414, "identity": 1.0, "score": 512.0, "evalue": 1.33e-178, "gene_start": 1, "gene_end": 275, "reference_start": 50, "reference_end": 324, "reference_sequence": "MTKKILSLTFKFNKKNKWVYIGLSLSFIISFIFCSLFLFDNYLFYNYFWMIFLLDFIIVCVSTLFILHLTFVSNQKSIYENTLNCRYFNKPKIVLAWSIYSFILLSAFSLLKSFAISSLHLYRDTNIKSFLLYFIGYFLSNLLIIIFIILVFASFIFITKSYKKSLVIATCILPFFTVVSLSSKTIEARANKKVEINKDIIVSGDKYYFLSDDEIKSKYNLNLISSIYFLPKNTINKYFDENFLTYKNNSDTKNYIFSTFNLSILKNNKKFDKDKFRIYHNDGEFFIKMSTNELANYLYSKLKKYFESGGVNLDKLLKKIKSNIWIKNNFTGNEYNLVMHLLGKDDFNFQYLQRDWNDNLWNAAKLKSNLNSMFKINFFDFLNHLYRSNQTKFNTTINMAEFYIVKDNNLIKYLDSNSKITDSDLNFISEKMVNFVGDKVIIDFNKYAQSELENSITTTITKFNLYFPEIDSKEKWKNLVYSSSNHLFIIKQTIDKMNELVSNLGNYQAFKFEENVNPKNFYDNTKIIKLNNLTYDLINLFLITFVILTLSILPIIFTFKRRKHE" }, "paralog": false, "identity": 0.9882352941176471, "score": 826.0, "evalue": 1.05e-304, "description": "Internal stop codon at 163599, 163614, 163707, 164535, 164628. Nonsense mutation around 163599, 163614, 163707, 164535, 164628.", "psc": { "uniref90_id": "UniRef90_C4XE51", "product": "ABC transporter permease", "uniref50_id": "UniRef50_C4XE51", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE51", "UniRef:UniRef50_C4XE51" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE51", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE51" ], "product": "ABC transporter permease" } }, "id": "OAIKOHHJFB_329", "locus": "GCA000209735_00298" }, { "type": "cds", "sequence": "contig_1", "start": 164672, "stop": 165418, "strand": "-", "frame": 1, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000B41DE", "UniRef:UniRef100_C4XE52", "UniRef:UniRef50_C4XE52", "UniRef:UniRef90_C4XE52" ], "nt": "ATGTTTTGGTTTCTTATTGAAAGATTTTTATATATAATTACATTTATGATAAAAATAGTTAATTTATCAAAAAAAGATAAAAAAAATCGATTGATTTTAAACAACTTAAATTTCAATATTCATGAAAATGAATTGCATGTTTTTTTGGGCAAAAATGGTGCTGGAAAAACAGTTACTTTAGGAATAATTTGTGGATTAGATTCAGAATTTTCTGGAAAAATTTTAAAAGATAATGTAGATATTTACAAATCAAATAAAACTAATAATTATTTGTTTGTTCCAACAGATATTAGATTTCCAAATCATTTAAAAGTTATTGATTTAATTAAATTTTATGTGAATGAAATTTTTGAATTAGAAACAAATGAATTATTTATAAATGAAACATTAAAAGAATTCCATTTAGATGAAAGAAAGGACTATCTTCCTAAAAAATTATCTTCTGGAGAAAAAAGAAAATTAATGCTATTAATATCTAAATTAGTAAAACCAAAATATTTATTCTTAGATGAACCAGAAGCTAATCTAGATCCTTATTCAAGAAATGAATTATTTAAACTTTTGAATATGTTAAAAAAAGAAGGAACTTCAATTTTCGCATCATCTCACTTAATATCTGAAGAATTGCAAGATATAGATAGAATAACAATTTTGCATAACAAAACTATCGTTCTAAGTGAAAAAGTAAAATCAGCAAAAAATTTAAAAATTATTTTTGATAATTTTAAGAGAGGTGGTTATGACTAA", "aa": "MFWFLIERFLYIITFMIKIVNLSKKDKKNRLILNNLNFNIHENELHVFLGKNGAGKTVTLGIICGLDSEFSGKILKDNVDIYKSNKTNNYLFVPTDIRFPNHLKVIDLIKFYVNEIFELETNELFINETLKEFHLDERKDYLPKKLSSGEKRKLMLLISKLVKPKYLFLDEPEANLDPYSRNELFKLLNMLKKEGTSIFASSHLISEELQDIDRITILHNKTIVLSEKVKSAKNLKIIFDNFKRGGYD", "aa_hexdigest": "da481b283756ebdcdf5fc56b9c84cf08", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000B41DE", "uniref100_id": "UniRef100_C4XE52", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000B41DE", "UniRef:UniRef100_C4XE52" ] }, "ips": { "uniref100_id": "UniRef100_C4XE52", "uniref90_id": "UniRef90_C4XE52" }, "psc": { "uniref90_id": "UniRef90_C4XE52", "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_C4XE52" }, "pscc": { "uniref50_id": "UniRef50_C4XE52", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE52" ], "product": "ABC transporter domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_330", "locus": "GCA000209735_00299" }, { "type": "cds", "sequence": "contig_1", "start": 166330, "stop": 166536, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEU7", "UniRef:UniRef90_C4XEU7" ], "nt": "ATGTCTTATACAGGAAATTTTGATACTTTTTCAACATTCTTTCAAAACTATGGATTAAATTATGACAAGTTAGAATCAGAAAAAAAACAAATAATAATTAATAAAAAATTATTACCAGAGAAAACGGCAAATTCTAAAATTTCTGAAACTAAAACTGAATCTTTGTTCAAAAAGATTAGTTCAAAATCTTTATTTGAAAAAATGTGA", "aa": "MSYTGNFDTFSTFFQNYGLNYDKLESEKKQIIINKKLLPEKTANSKISETKTESLFKKISSKSLFEKM", "aa_hexdigest": "d14ed2add281c7a34f2d13d149b41d1e", "start_type": "ATG", "rbs_motif": "AGGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 166330, "stop": 166638, "observations": { "mutation": [ 166534 ], "stop": [ 166534 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEU7", "query_cov": 1.0, "subject_cov": 0.6666666666666666, "identity": 1.0, "score": 126.0, "evalue": 1.49e-36, "gene_start": 1, "gene_end": 68, "reference_start": 1, "reference_end": 68, "reference_sequence": "MSYTGNFDTFSTFFQNYGLNYDKLESEKKQIIINKKLLPEKTANSKISETKTESLFKKISSKSLFEKMWKSKKYLNFADKALIFLSTLFKMKIQYQKEFLMN" }, "paralog": false, "identity": 0.9901960784313726, "score": 198.0, "evalue": 2.93e-67, "description": "Internal stop codon at 166534. Nonsense mutation around 166534.", "psc": { "uniref90_id": "UniRef90_C4XEU7", "uniref50_id": "UniRef50_C4XEU7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEU7", "UniRef:UniRef50_C4XEU7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEU7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEU7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7940.015899999999, "isoelectric_point": 9.398668479919433 }, "id": "OAIKOHHJFB_331", "locus": "GCA000209735_00300" }, { "type": "gap", "sequence": "contig_1", "start": 166642, "stop": 166642, "strand": ".", "length": 1, "id": "OAIKOHHJFB_1799" }, { "type": "gap", "sequence": "contig_1", "start": 166746, "stop": 166845, "strand": ".", "length": 100, "id": "OAIKOHHJFB_1800" }, { "type": "cds", "sequence": "contig_1", "start": 166961, "stop": 167311, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGGGCATTAGCCCTCCCTAAAATATTTGGCGACTTGTCGCCCTTTCTAAAATATTATTTTAATTTTCCTATTTTTTTGAAAAACAAATTTCTATTCTTGTTACTTTCTCAGTTTCTCTTTTGTTTTCCATATAATAATTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCGTACATTTCCAAAGCAGATAAATATCCAAAAATTTCTCTTGGACGATTGTTTATTTCATTTTGTACTTTTAGAATTTCTTCTTCAGTAATATCATCAAAATTAGTTTTTTTCTTAAAAAATCTTCTAACTAAACCATTGAAATTTTCATTTGAACCCCTTTGA", "aa": "MRALALPKIFGDLSPFLKYYFNFPIFLKNKFLFLLLSQFLFCFPYNNLSIPVEFQGFSAFNFSYISKADKYPKISLGRLFISFCTFRISSSVISSKLVFFLKNLLTKPLKFSFEPL", "aa_hexdigest": "6a907dccef0cbbba0bbf260e0f56003e", "start_type": "GTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13576.067399999976, "isoelectric_point": 9.912288093566893 }, "id": "OAIKOHHJFB_332", "locus": "GCA000209735_00301" }, { "type": "cds", "sequence": "contig_1", "start": 167446, "stop": 167994, "strand": "-", "frame": 2, "gene": "rsmD", "product": "16S rRNA (guanine(966)-N(2))-methyltransferase RsmD", "db_xrefs": [ "EC:2.1.1.171", "GO:0031167", "GO:0052913", "KEGG:K08316", "RefSeq:WP_013526667.1", "SO:0001217", "UniParc:UPI0001F59D2D", "UniRef:UniRef100_C4XE55", "UniRef:UniRef50_A0A3P8MDG1", "UniRef:UniRef90_C4XE55" ], "nt": "ATGTTAAGAATTATCAGTGGAAAATATCGTCATTTAAATATAGAACAACCTAAAACTTTGGAAACAAGACCAACTATGGATCGTGTTCGTGAAGCTATTTTTTCATCAATAAGATTTAACTTAGAAGGTGCTTGCATTTTAGATCTTTTTGCAGGTAGTGGTGCTTTTAGTTTTGAAGCATTAAGCAACTATGCGGTAAAAGCAATAGCTGTTGATAATAATAATGAAGCAATTAAAGCAATAAGAAATAATGCTTTAAAACTAAAAGCTGAAAATATCGATATTGTAAAAGATGATGTTTTGAATTATTTACAAAAGAATTCGGGACGTAAATTTGATTTTATTTTTATTGATGCTCCATACAAGGAATATCAATTGATTAATAAATCTCTTGAATTAATAGTTGATAATAAATTTTTAACTAAAAATGGTTTAATTATTTTAGAAACTGACAATTTCTTAATGATTGATATTCCAAAAAAGCTTACAATACAAAAACAAAAACAATATGGAAAAGTAAATATTTTGTTTATCTCAAATAATAATTAA", "aa": "MLRIISGKYRHLNIEQPKTLETRPTMDRVREAIFSSIRFNLEGACILDLFAGSGAFSFEALSNYAVKAIAVDNNNEAIKAIRNNALKLKAENIDIVKDDVLNYLQKNSGRKFDFIFIDAPYKEYQLINKSLELIVDNKFLTKNGLIILETDNFLMIDIPKKLTIQKQKQYGKVNILFISNNN", "aa_hexdigest": "39e8e1dbd7c392c265564285e26a746c", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001F59D2D", "ncbi_nrp_id": "WP_013526667.1", "uniref100_id": "UniRef100_C4XE55", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59D2D", "RefSeq:WP_013526667.1", "UniRef:UniRef100_C4XE55" ] }, "ips": { "uniref100_id": "UniRef100_C4XE55", "uniref90_id": "UniRef90_C4XE55" }, "psc": { "uniref90_id": "UniRef90_C4XE55", "gene": "rsmD", "product": "16S rRNA (guanine(966)-N(2))-methyltransferase RsmD", "ec_ids": [ "2.1.1.171" ], "uniref50_id": "UniRef50_A0A3P8MDG1", "kegg_orthology_id": "K08316", "go_ids": [ "GO:0031167", "GO:0052913" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MDG1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDG1" ], "product": "N6-adenine-specific methylase" }, "genes": [ "rsmD" ], "id": "OAIKOHHJFB_333", "locus": "GCA000209735_00302" }, { "type": "cds", "sequence": "contig_1", "start": 168065, "stop": 168634, "strand": "+", "frame": 2, "gene": "def", "product": "peptide deformylase", "db_xrefs": [ "EC:3.5.1.88", "GO:0036211", "GO:0042586", "KEGG:K01462", "RefSeq:WP_013526668.1", "SO:0001217", "UniParc:UPI0001F59D2E", "UniRef:UniRef100_C4XE56", "UniRef:UniRef50_Q98PN3", "UniRef:UniRef90_C4XE56" ], "nt": "ATGAAATATGAAATAAAACTAGTTGAATTACCTGAAAAAATTTTAAGGAAGAAATCAAAAGAAGTCAAAATTCCTCTTTCAAAAGAAGATGAAGAATTAGCTAAAAAAATGATTTATCACATAGACGAAAGCCAGAAAGAAGATTCTAAATTAGGTTTTAGACCTGGTGTTGGAGTTGCAGCTATTCAATATGGAATTCCAAAAAGAATGTTTTATATAAACATTAACAATGAAACAATAAATGGAGCTAAAATTGAAGACTTTAGAGATGTATTAATTAATCCAAAAGTTTTAGCAACCAGCGAATATGAAGTTTCATTACCTGGCGAAGGTTGTTTAAGTGTTGGGGACAACATTAAAAATCAAGAAGGCTATGTTTATCGTAAGAAAAGAATCGTTTTAGAAGCTTATTCATATTTTGAAAAGAAAGTGAAGCATTATGATTTAGTTGATTATGCAGCAATTGTTGCGCAACATGAATTTGATCACCTGGAAGGAAAATTATTCATCGATAGAATTAACAAAAAAGATCCTTTTGTAATCAAAGATGATTCAGAAATTTTAGAATAA", "aa": "MKYEIKLVELPEKILRKKSKEVKIPLSKEDEELAKKMIYHIDESQKEDSKLGFRPGVGVAAIQYGIPKRMFYININNETINGAKIEDFRDVLINPKVLATSEYEVSLPGEGCLSVGDNIKNQEGYVYRKKRIVLEAYSYFEKKVKHYDLVDYAAIVAQHEFDHLEGKLFIDRINKKDPFVIKDDSEILE", "aa_hexdigest": "be95a1707f560b5646e6a1050d46db22", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59D2E", "ncbi_nrp_id": "WP_013526668.1", "uniref100_id": "UniRef100_C4XE56", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59D2E", "RefSeq:WP_013526668.1", "UniRef:UniRef100_C4XE56" ] }, "ips": { "uniref100_id": "UniRef100_C4XE56", "uniref90_id": "UniRef90_C4XE56" }, "psc": { "uniref90_id": "UniRef90_C4XE56", "gene": "def", "product": "peptide deformylase", "ec_ids": [ "3.5.1.88" ], "uniref50_id": "UniRef50_Q98PN3", "kegg_orthology_id": "K01462", "go_ids": [ "GO:0036211", "GO:0042586" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PN3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PN3" ], "product": "Peptide deformylase" }, "genes": [ "def" ], "id": "OAIKOHHJFB_334", "locus": "GCA000209735_00303" }, { "type": "cds", "sequence": "contig_1", "start": 168724, "stop": 169434, "strand": "+", "frame": 1, "gene": "cvpA", "product": "CvpA family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE57", "UniRef:UniRef90_C4XE57" ], "nt": "TTGATAGTCCTTTTGGTCGCAATTGCTATTGGGGCTAAAAGAGGATTTTATGCAGGTTTAATGTCTTTAGGATTTAACTTTTTAGGCTTTATCTTAGGTATAATTGTGGCTCCTCTTATTATTGAGCAATCAATTAGTACCAAACAGTCTAATTTAAATTTAATGAATAAACTTAAAACACTAAAACCGATGTTAGTTGGGGTCATAATTTTAGGTTTCACAGCAATTTTTGGTCTAATTGGTGAAATAGTATTTCTTTGTCTAAAAGCATTTTTCAAAAGACCAATTATTAAAAGAAAAAATAAGGGCGAAAGCGTAATATTCTACCGTTCAATGGGAGCTATCGCCTCAGGTTTAGCTTTATTACCAGGTGCAATTCTAATGACAAACTTAGTAGGTTTTGTTGATCAAAACTCTGCAATTAACAAAATCAATAACAAAGTTCTCAATTTTATTACAATTGACCAAGCAAAAGGAGTTAGTGAATATGCTCCAGGTTTAATGGCTTTAAGCAAACTTAAAAGAGATGACCAACTTAAAAAAGATGTTAATAAATTCTTCCAATCAATTTTTGATTTAAACAATTATGTTTTTCAAATGACTTCTAAAGGTACTAGCCTAAAAGCAACCGATGAGTCAATTAATGATCAAAATATAAAGACAGATAATTCGAATTTACGTCAAATTCATTTTGTTCCTCTTGCAAGTTGA", "aa": "MIVLLVAIAIGAKRGFYAGLMSLGFNFLGFILGIIVAPLIIEQSISTKQSNLNLMNKLKTLKPMLVGVIILGFTAIFGLIGEIVFLCLKAFFKRPIIKRKNKGESVIFYRSMGAIASGLALLPGAILMTNLVGFVDQNSAINKINNKVLNFITIDQAKGVSEYAPGLMALSKLKRDDQLKKDVNKFFQSIFDLNNYVFQMTSKGTSLKATDESINDQNIKTDNSNLRQIHFVPLAS", "aa_hexdigest": "91c3aba2a0cf73cb27aa852bf5b278e7", "start_type": "TTG", "rbs_motif": null, "genes": [ "cvpA" ], "truncated": "both", "pseudogene": { "start": 168631, "stop": 169737, "observations": { "mutation": [ 168670, 168694, 169432, 169474, 169603 ], "stop": [ 168670, 168694, 169432, 169474, 169603 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE57", "query_cov": 1.0, "subject_cov": 0.48459958932238195, "identity": 0.996, "score": 439.0, "evalue": 1.41e-151, "gene_start": 1, "gene_end": 236, "reference_start": 32, "reference_end": 267, "reference_sequence": "MINEVFMRTYEDNWKALATNGWCVLISISVFLIVLLVAIAIGAKRGFYAGLMSLGFNFLGFILGIIVAPLIIEQSISTKQSNLNLMNKLKTLKPMLVGVIILGFTAIFGLIGEIVFLCLKAFFKRPIIKRKNKGESVIFYRSMGAIASGLALLPGAILMTNLVGFVDQNSAINKINNKVLNFITIDQAKGVSEYAPGLMALSKLKRDDQLKKDVNKFFQSIFDLNNYVFQMTSKGTSLKATDESINDQNIKTDNSNLRQIHFVPLASWSDEEIERLPKVIDWLNYCFNDEASFDLLNNSYNYPTGETIKYYFKGVYSNDPSKATWNTKVLKYGNVYYYKKMNFGGPDSWSPATTTYAESLENKFDFKTMFSWNIFKWSQTLRTLSKIDLDSKFEVSDSKVEDFTKIKKIKNKDIQFKLNVENEVVRNKIKSIFYKLISLENEISNTKIDESKVLGFSTDSANKNQINDNTIKRFQLYFDNLLDTIMG" }, "paralog": false, "identity": 0.9836956521739131, "score": 706.0, "evalue": 5.55e-259, "description": "Internal stop codon at 168670, 168694, 169432, 169474, 169603. Nonsense mutation around 168670, 168694, 169432, 169474, 169603.", "psc": { "uniref90_id": "UniRef90_C4XE57", "product": "CvpA family protein", "uniref50_id": "UniRef50_C4XE57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE57", "UniRef:UniRef50_C4XE57" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE57" ], "product": "CvpA family protein" } }, "id": "OAIKOHHJFB_335", "locus": "GCA000209735_00304" }, { "type": "cds", "sequence": "contig_1", "start": 169936, "stop": 170094, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTCTCTTGAAAATGAGATATCAAACACAAAAATCGACGAATCAAAAGTATTAGGTTTCTCAACTGATTCAGCCAACAAAAATCAAATTAATGACAACACAATTAAAAGATTCCAATTGTATTTTGACAACTTACTTGATACAATTATGGGCTAA", "aa": "MISLENEISNTKIDESKVLGFSTDSANKNQINDNTIKRFQLYFDNLLDTIMG", "aa_hexdigest": "47c6fdda95958ade5fbdcc9d29a5f90a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5940.5796, "isoelectric_point": 4.4576208114624025 }, "id": "OAIKOHHJFB_336", "locus": "GCA000209735_00305" }, { "type": "cds", "sequence": "contig_1", "start": 170245, "stop": 170361, "strand": "+", "frame": 1, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A829GPU2", "UniRef:UniRef90_UPI0020BDD874" ], "nt": "ATGCAAAAATATGACGAATCAAGTATACAACAATTAAAAGGCTTAGAAGCTGTTCGAAAACGTCCAGGGATGTACATTGGAAGTACAGATGTTAATGGACTTCATCACTTAATTTGA", "aa": "MQKYDESSIQQLKGLEAVRKRPGMYIGSTDVNGLHHLI", "aa_hexdigest": "b449758f9a54df4fdf5a724069df0dba", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 170254, "stop": 170385, "observations": { "mutation": [ 170359 ], "stop": [ 170359 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0020BDD874", "query_cov": 0.9210526315789473, "subject_cov": 0.660377358490566, "identity": 0.8, "score": 60.8, "evalue": 1.33e-11, "gene_start": 4, "gene_end": 38, "reference_start": 11, "reference_end": 45, "reference_sequence": "MEQKQMPENAYDESQIQVLEGLEAVRKRPGMYIGSTSGKGLHHLVWEIVDNSI" }, "paralog": false, "identity": 0.813953488372093, "score": 72.0, "evalue": 4.97e-19, "description": "Internal stop codon at 170359. Nonsense mutation around 170359.", "psc": { "uniref90_id": "UniRef90_UPI0020BDD874", "uniref50_id": "UniRef50_A0A829GPU2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0020BDD874", "UniRef:UniRef50_A0A829GPU2" ] }, "pscc": { "uniref50_id": "UniRef50_A0A829GPU2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A829GPU2" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" } }, "id": "OAIKOHHJFB_337", "locus": "GCA000209735_00306" }, { "type": "cds", "sequence": "contig_1", "start": 170452, "stop": 170748, "strand": "+", "frame": 1, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920EUL8" ], "nt": "ATGGATAATGGTCGTGGAATTCCAGTTGGTAAAACTCCTTCAGGTAAAAGTGCAGTTGAATTAGTTTTCACTGAACTTCATGCTGGTGGAAAATTTAATGAAGGAGCTTATAAAACTAGTGGTGGACTTCATGGTGTTGGGTCTTCAGTAGTTAATGCTTTAAGCACAAAATTAATTGTCACAGTTTACCGCGACAAAAAAATCTATGAAACAATTTTTGAAAATGGTGATAATATTGTCCAAAGAACACTCGAAGTTGGAAATACAAACAAACATGGTACCATGGTTGAATTTTGA", "aa": "MDNGRGIPVGKTPSGKSAVELVFTELHAGGKFNEGAYKTSGGLHGVGSSVVNALSTKLIVTVYRDKKIYETIFENGDNIVQRTLEVGNTNKHGTMVEF", "aa_hexdigest": "a1a737656f6194c0eeefed80e0d6070d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A920EUL8", "query_cov": 0.8163265306122449, "subject_cov": 0.8333333333333334, "identity": 0.588, "score": 102.0, "evalue": 1.67e-26, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0A920EUL8" }, "pscc": { "uniref50_id": "UniRef50_A0A920EUL8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920EUL8" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "OAIKOHHJFB_338", "locus": "GCA000209735_00307" }, { "type": "cds", "sequence": "contig_1", "start": 170884, "stop": 171315, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00204.31" ], "nt": "ATGTATGAAGATGGTCTCCATGCTTTTGTTGATTTCATTAATGATTCAAAAAATGCACTTGGGGACATCAAAACTTTTAAAGAAACAAAAAGAGATATTGAAGTCGAATGCGGCTTCCAATACACAGATAGTTATAATGAAACAGTTTTAAGTTTTGTTAATAATGTTAAGACCAAAGATGGTGGTACACATGAAATAGGACTTAAGTCTGCTTTAACTAAAACATTTAATGAATTTGCTTTAGAAGAAAAAGCTTTAAAAGGCAAAAACACTTTTGATGGTGATGACATTAGAGAGGGTCTAACTATTATTCTTTCAGTTAAGATTCCTGAAAAATTACTTGAATTTGTTAGTCAAACAAAAGAAAAGTTAGGAACACCAGAAGCTAAAGGCGTAGTTGAAGAAATAGTTTCTAAAAACTTTAAAATTTGA", "aa": "MYEDGLHAFVDFINDSKNALGDIKTFKETKRDIEVECGFQYTDSYNETVLSFVNNVKTKDGGTHEIGLKSALTKTFNEFALEEKALKGKNTFDGDDIREGLTIILSVKIPEKLLEFVSQTKEKLGTPEAKGVVEEIVSKNFKI", "aa_hexdigest": "d3567811b9e0fe67cd93cb1d3264be85", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00204.31", "name": "DNA_gyraseB", "length": 142, "aa_cov": 0.965034965034965, "hmm_cov": 0.9859154929577465, "evalue": 4.15287349332824e-46, "score": 152.67640686035156, "start": 5, "stop": 142 } ], "seq_stats": { "molecular_weight": 16044.96759999999, "isoelectric_point": 5.041698265075683 }, "id": "OAIKOHHJFB_339", "locus": "GCA000209735_00308" }, { "type": "cds", "sequence": "contig_1", "start": 171481, "stop": 171888, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTGCTGAAAAAGAATTATTCTTAGTCGAAGGTGACTCTGCTGGAGGTAGTGCAAAACTTGGACGTGATCGTAAATATCAAGCAATTTTACCTTTAAGAGGAAAAGTTATTAATACTGAAAAAGCTCGTCTTTTTGAGATTTTAAAAAATGAAGAAATTGGAACAATAATTAATACAATCGGCGCTGGTGTTGGTGACGAATTTGACCTTAAAAAAGCTCAATACGGAAAAGTTGTTATTATGACCGATGCCGATACTGATGGTGCTCATATTCAAATTTTATTACTTACTTTCTTCTTTAGACACATGCGCCAATTAGTTGAAAATGGAATGGTTTATATAGCTCTTCCACCTTTATTTAAATTAACTTCAACTAGAGGTAAAAAAGAAGTTTTATATGCTTGA", "aa": "MPAEKELFLVEGDSAGGSAKLGRDRKYQAILPLRGKVINTEKARLFEILKNEEIGTIINTIGAGVGDEFDLKKAQYGKVVIMTDADTDGAHIQILLLTFFFRHMRQLVENGMVYIALPPLFKLTSTRGKKEVLYA", "aa_hexdigest": "e22811784afa56e0e4e29a121b6eb988", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002AC415C3", "query_cov": 0.9629629629629629, "subject_cov": 0.8609271523178808, "identity": 0.5770000000000001, "score": 162.0, "evalue": 6.93e-49, "valid": false, "product": "toprim domain-containing protein", "uniref50_id": "UniRef50_A0A353NP07" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15027.384699999982, "isoelectric_point": 8.829992866516115 }, "id": "OAIKOHHJFB_340", "locus": "GCA000209735_00309" }, { "type": "cds", "sequence": "contig_1", "start": 172000, "stop": 172110, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCCTATAACTAGAACTCTTGTACAAGTAAAAATAGAAGATGCTGCTTTAGCTGAACGTCGTGTTTCAACACTTATGGGTGACAATGTTGAACCACGTAAAGAATGA", "aa": "MNPITRTLVQVKIEDAALAERRVSTLMGDNVEPRKE", "aa_hexdigest": "8cc26119281ffd9c9602e0f30e1db464", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4081.676299999999, "isoelectric_point": 6.16506404876709 }, "id": "OAIKOHHJFB_341", "locus": "GCA000209735_00310" }, { "type": "cds", "sequence": "contig_1", "start": 172171, "stop": 172371, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00521.26" ], "nt": "ATGAATAAAAATCAAGAAAACAAATTAAATGATTTATTAAGCAAGATTATTAAAGAAAATCTTGATACTATAATGGCTGACCGTTTTAGTCGTTATTCAAAATATATTATTCAACAACGTGCTTTACCTGATGTTAGAGATGGACTTAAACCTGTTCAAAGAAGAATTCTTTATTCTATGTATGAATTAGGTTTGCAATGA", "aa": "MNKNQENKLNDLLSKIIKENLDTIMADRFSRYSKYIIQQRALPDVRDGLKPVQRRILYSMYELGLQ", "aa_hexdigest": "488e542d0a74fbc41414112f39c48729", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00521.26", "name": "DNA_topoisoIV", "length": 24, "aa_cov": 0.36363636363636365, "hmm_cov": 1.0, "evalue": 2.2717028864865335e-12, "score": 42.56110763549805, "start": 40, "stop": 63 } ], "seq_stats": { "molecular_weight": 7887.083800000003, "isoelectric_point": 9.62727336883545 }, "id": "OAIKOHHJFB_342", "locus": "GCA000209735_00311" }, { "type": "cds", "sequence": "contig_1", "start": 172486, "stop": 173916, "strand": "+", "frame": 1, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D5N2", "UniRef:UniRef90_C4XE59" ], "nt": "ATGGGACATACACTTTTAGAAATGCATGGGAATGTTGGATCTATTGATGATGACCCTGCAGCTGCAATGCGTTATACTGAAGTTAGATTAGCTGCTTTAGCTGACTTAGTTATTGGTGACTTAAAGAAAAATACAGTTAAGTTTGCTCCTAACTTTGATGATAGTGAAAAAGAGCCAGTTGTTTTACCTTCACTTATTCCAAATTTATTATTAAATGGGGCTAAAGGTATAGCTTCAGGTTTTGCTACTGAAATGCCACCGCACAACTTAAATGAAATATTAGATGCCTCAATTGCAAAAATTAAAAACCCTGATATTCAGCTTTCAAAATTATTTAAATATGTAAAGGGACCCGATTTTCCAACAGGTGGTGTTGTCTATGGACAACAAGGAATTTATGAAGCTTTTGAAAGAGGAAGAGGAAGAATTACTCTTGTTTCAAGATATAGAACTTTTGAAGACAAAAAAAATAAATTTATCGAAATTAATGAAATTCCTTATGGTGTTATTAAATCAAAATTAGTTCATGATATTGATTTAATTATTGCTAAAGAAAGTGTTAGTGGTCTACTTGAAATTAAGGATCAATCAGACCGAAATGGAATTAGCATTCTTATCACTTTAGATAAAAAAGCCAATGAAGAAGTTATTTTAAATTACTTGCTTCAAAAAACTGAAATGCAAATTTACTATACCTATAATAATGTGGCTATTAAAAATAATTCACCACAAACTTTAGGTTTAAATGATTTATTAAATTGCTATCTTGATCACGTTCGTGATGTCAAAAGAAAAACACTCGAATTTGATTTAGTTAAATATAAAGCTAGATTAGAAATTGTTTTAGGTTTTCTTAAAGTTGCAGAAATTACTGATGAAGTAATCGCAGTTATTAGAAAAAGTGAAAACTCAAAAGCTGGTGTTATTCAAAACTTAATGAACCACTTTGGTTTTACCTTGAATCAAGCAACAGCTATTGCTGAATTAAGACTTTACAAACTTTCCAAAACTGATAAAGAAGCTTTCTTATTAGAAAAAGAAGAATTAGAAAAACAAATCAAACATGTCGAAATGTTACTAAATGATAAAGAAGCTTTTGATCAATACTTAATTGAAACATTGCAAGAAATTAAAAAACAATTTGGCCGTCCAAGAAGAACAACTATTACTGAAGAAGCATTTAAAGTAAGTTACAATGAAGCTGACTTAGTTAAAGAAGAAGAAACTTATATTGGAGTTACAAAACATGGCTACTTAAAAAGAATGAGCAACAAAGTTGTGAACTCAAATGACTTCTTAACTTATGGAATTAAAGAAGGCGACAAAATTATTTGTTTTGAAAAAAGCAACACAATTAACAATTTATTGCTTTTCACAAATTTAGGTAATTATGCAATGGTACCTATTTATAAAATAGAAGAAAGCAAATGA", "aa": "MGHTLLEMHGNVGSIDDDPAAAMRYTEVRLAALADLVIGDLKKNTVKFAPNFDDSEKEPVVLPSLIPNLLLNGAKGIASGFATEMPPHNLNEILDASIAKIKNPDIQLSKLFKYVKGPDFPTGGVVYGQQGIYEAFERGRGRITLVSRYRTFEDKKNKFIEINEIPYGVIKSKLVHDIDLIIAKESVSGLLEIKDQSDRNGISILITLDKKANEEVILNYLLQKTEMQIYYTYNNVAIKNNSPQTLGLNDLLNCYLDHVRDVKRKTLEFDLVKYKARLEIVLGFLKVAEITDEVIAVIRKSENSKAGVIQNLMNHFGFTLNQATAIAELRLYKLSKTDKEAFLLEKEELEKQIKHVEMLLNDKEAFDQYLIETLQEIKKQFGRPRRTTITEEAFKVSYNEADLVKEEETYIGVTKHGYLKRMSNKVVNSNDFLTYGIKEGDKIICFEKSNTINNLLLFTNLGNYAMVPIYKIEESK", "aa_hexdigest": "ed2c4de08bd2fa5763c72c9c2d79f045", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 172186, "stop": 174219, "observations": { "mutation": [ 172369, 172480, 173914 ], "stop": [ 172369, 172480, 173914 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE59", "query_cov": 1.0, "subject_cov": 0.5554259043173863, "identity": 1.0, "score": 923.0, "evalue": 0.0, "gene_start": 1, "gene_end": 476, "reference_start": 107, "reference_end": 582, "reference_sequence": "MMNKNQENKLNDLLSKIIKENLDTIMADRFSRYSKYIIQQRALPDVRDGLKPVQRRILYSMYELGLQWNKPFKKSARVVGDVIGKYHPHGDSSIYEAMVRMAQDWKMGHTLLEMHGNVGSIDDDPAAAMRYTEVRLAALADLVIGDLKKNTVKFAPNFDDSEKEPVVLPSLIPNLLLNGAKGIASGFATEMPPHNLNEILDASIAKIKNPDIQLSKLFKYVKGPDFPTGGVVYGQQGIYEAFERGRGRITLVSRYRTFEDKKNKFIEINEIPYGVIKSKLVHDIDLIIAKESVSGLLEIKDQSDRNGISILITLDKKANEEVILNYLLQKTEMQIYYTYNNVAIKNNSPQTLGLNDLLNCYLDHVRDVKRKTLEFDLVKYKARLEIVLGFLKVAEITDEVIAVIRKSENSKAGVIQNLMNHFGFTLNQATAIAELRLYKLSKTDKEAFLLEKEELEKQIKHVEMLLNDKEAFDQYLIETLQEIKKQFGRPRRTTITEEAFKVSYNEADLVKEEETYIGVTKHGYLKRMSNKVVNSNDFLTYGIKEGDKIICFEKSNTINNLLLFTNLGNYAMVPIYKIEESKWKDFGVHLSDFVELVSGEELVGAINVTDFNIQNYVCLFTKEAQGKRVVLKEFEVSRPNKTFTAMKLRKGDELVGAKLSNGYKDVLLITKRGMASLYTENDVQIYGTKSNGTKSCYMPPMDSIVAFAMVEAHETLAMLANETYVKRIHVSNITKSGKKNLGKPIFAQLKTKPYIVTDAYPMTVDDYIFVTNDENQVLLERIVEYPITSINEGFSKIKINNSAALSFKKNETSSSSKPVEIKFEQPAPTEEAKFEKSEETIEVLSKRVEELLKKFGK" }, "paralog": false, "identity": 0.9955686853766618, "score": 1316.0, "evalue": 0.0, "description": "Internal stop codon at 172369, 172480, 173914. Nonsense mutation around 172369, 172480, 173914.", "psc": { "uniref90_id": "UniRef90_C4XE59", "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_I5D5N2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE59", "UniRef:UniRef50_I5D5N2" ] }, "pscc": { "uniref50_id": "UniRef50_I5D5N2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D5N2" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" } }, "id": "OAIKOHHJFB_343", "locus": "GCA000209735_00312" }, { "type": "cds", "sequence": "contig_1", "start": 174103, "stop": 174741, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACTTAGAAAAGGTGATGAATTAGTTGGAGCTAAACTTTCAAATGGATATAAAGATGTTTTATTAATTACCAAACGTGGTATGGCTTCTTTATATACTGAAAATGATGTTCAAATTTATGGTACCAAATCAAATGGAACTAAGAGTTGTTATATGCCACCAATGGATTCAATTGTTGCTTTTGCAATGGTTGAAGCTCATGAAACTTTAGCAATGTTAGCTAATGAAACTTATGTTAAAAGAATTCATGTAAGCAACATCACTAAATCAGGCAAAAAGAATTTAGGTAAACCAATTTTTGCTCAACTTAAAACAAAACCTTATATTGTTACAGATGCCTATCCAATGACAGTTGATGATTACATTTTTGTAACAAATGATGAAAACCAAGTTTTACTCGAAAGAATTGTTGAATATCCTATTACTTCTATTAATGAAGGATTTTCAAAAATTAAGATCAATAATTCAGCTGCATTATCATTTAAAAAGAATGAAACTTCTTCATCTTCTAAACCTGTAGAAATCAAATTTGAACAACCAGCTCCTACTGAAGAAGCTAAGTTTGAAAAATCAGAAGAAACAATTGAAGTACTTTCAAAAAGAGTTGAAGAACTCTTGAAAAAATTTGGCAAATAA", "aa": "MKLRKGDELVGAKLSNGYKDVLLITKRGMASLYTENDVQIYGTKSNGTKSCYMPPMDSIVAFAMVEAHETLAMLANETYVKRIHVSNITKSGKKNLGKPIFAQLKTKPYIVTDAYPMTVDDYIFVTNDENQVLLERIVEYPITSINEGFSKIKINNSAALSFKKNETSSSSKPVEIKFEQPAPTEEAKFEKSEETIEVLSKRVEELLKKFGK", "aa_hexdigest": "db991e1d924fe66d1fc9ba1eb9d19c86", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23818.17920000002, "isoelectric_point": 8.704215049743652 }, "id": "OAIKOHHJFB_344", "locus": "GCA000209735_00313" }, { "type": "cds", "sequence": "contig_1", "start": 174840, "stop": 175115, "strand": "+", "frame": 3, "gene": null, "product": "Phosphoesterase", "db_xrefs": [ "KEGG:K07095", "SO:0001217", "UniRef:UniRef50_C4XE60", "UniRef:UniRef90_C4XE60" ], "nt": "ATGGAAAAAGAAATAAAAATTTTAGTAGTCAGTGATATTCACGGGAAAAAATCAATAGCTGACAAAATCTTAAAAAATGAGGACTATGATTATGCAATAAGTTGTGGCGATCATTTACTAGATAAAGACTATATGATAAGCAATTTTGATTATTGCGTTGATGGTAATAATGATATTTATTTTGACAAATATTGCTTAAACAAGCCTACTTTAGATTTCACTTTAGCTGATTATAAATTTCATGTTGAACATGGTCATATGATCGGAGATTATTGA", "aa": "MEKEIKILVVSDIHGKKSIADKILKNEDYDYAISCGDHLLDKDYMISNFDYCVDGNNDIYFDKYCLNKPTLDFTLADYKFHVEHGHMIGDY", "aa_hexdigest": "afec43428a0c5d56c07ac79c75d19c96", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 174798, "stop": 175364, "observations": { "mutation": [ 174807, 175113, 175266 ], "stop": [ 174807, 175113, 175266 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE60", "query_cov": 1.0, "subject_cov": 0.4789473684210526, "identity": 1.0, "score": 194.0, "evalue": 6.67e-62, "gene_start": 1, "gene_end": 91, "reference_start": 17, "reference_end": 107, "reference_sequence": "MWIIFWFYFIILKEKIMEKEIKILVVSDIHGKKSIADKILKNEDYDYAISCGDHLLDKDYMISNFDYCVDGNNDIYFDKYCLNKPTLDFTLADYKFHVEHGHMIGDYWSLRTPNVLFDETKNIDCDFLICGHSHFPIYYKDVKTHKILLNPGSPSLPRWNSKPSYAIITIKNEKKYENVEVIFKEVNEKS" }, "paralog": false, "identity": 0.9840425531914894, "score": 384.0, "evalue": 7.16e-139, "description": "Internal stop codon at 174807, 175113, 175266. Nonsense mutation around 174807, 175113, 175266.", "psc": { "uniref90_id": "UniRef90_C4XE60", "product": "Phosphoesterase", "uniref50_id": "UniRef50_C4XE60", "kegg_orthology_id": "K07095", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE60", "UniRef:UniRef50_C4XE60", "KEGG:K07095" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE60", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE60" ], "product": "Phosphoesterase" } }, "id": "OAIKOHHJFB_345", "locus": "GCA000209735_00314" }, { "type": "cds", "sequence": "contig_1", "start": 175436, "stop": 175600, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGAGTAACAAAAAAAATAAACCTCAATATTTATTTGCTATTGACCTTGATGGAACACTTTTAGCATCAAGTGCAACAGGTGAAGTTCATAATACAACAGCTGAAGCAATAGCAAGAGCCAAAAAAGAAGGCCACATTGTTTGTGCTTTAACTGGTAGACCATGA", "aa": "MSNKKNKPQYLFAIDLDGTLLASSATGEVHNTTAEAIARAKKEGHIVCALTGRP", "aa_hexdigest": "c214cadee41183746f14a728cbb58d16", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 43, "aa_cov": 0.7962962962962963, "hmm_cov": 0.9534883720930233, "evalue": 3.491002087420403e-10, "score": 36.10066223144531, "start": 12, "stop": 54 } ], "seq_stats": { "molecular_weight": 5739.4995, "isoelectric_point": 8.979430580139162 }, "id": "OAIKOHHJFB_346", "locus": "GCA000209735_00315" }, { "type": "cds", "sequence": "contig_1", "start": 176150, "stop": 176368, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGTTTAAAGTTGCTAATATTTCTGTAGCTGTTGGTAATGCACCAGATGCAGTTAAAAAATATGCAACAATCAAATTAAAGAAAACAAACCGCCAGGGAGCAGTTGGTGAATACATCAATAAATTCCTTGATAATCCTGAAAAAGAAATAGAAAAAAGTAATAAAGTTATGGCTAAATTAATTAAACGTCGTGACGAAGAAGCAGATTTGGAAGACTAA", "aa": "MFKVANISVAVGNAPDAVKKYATIKLKKTNRQGAVGEYINKFLDNPEKEIEKSNKVMAKLIKRRDEEADLED", "aa_hexdigest": "0a12320d2db7049ce71bfbd74952a03b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 39, "aa_cov": 0.5416666666666666, "hmm_cov": 0.9743589743589743, "evalue": 1.7449923944756713e-07, "score": 27.257429122924805, "start": 1, "stop": 39 } ], "seq_stats": { "molecular_weight": 8123.280099999999, "isoelectric_point": 9.222154045104979 }, "id": "OAIKOHHJFB_347", "locus": "GCA000209735_00316" }, { "type": "cds", "sequence": "contig_1", "start": 176371, "stop": 176802, "strand": "+", "frame": 1, "gene": null, "product": "Ribonuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6M4JBT7", "UniRef:UniRef90_C4XE62" ], "nt": "ATGAAATTTCTTGAATACAATTTTTCTTTGGATATTAAAGACAAAAAACTCATTGGAGTTGACGAAGTAGGAGTGGGCGATTATTTTGGTCCACTTTGTTGTGCTGCAGTCTTTATTCCTCAAGATAAGATTCAAGAAGTTATTGATTTAGGTGTCAAAGATAGCAAAAAATTAAGTGATAAACAAATTAAAATGATGGCTTATAAACTTAAAAAAATTGTTAAGTATGCAATTCATCATTTAAGTCCAAAAGGTTACAATACATTAAATAAAACTTACAATGGAAATTTTTTAAAAATGTTTACACATTTAAGTGCAATTAGTTCACTTCAAGAAAAAATAGGCGCTGTAGATTATGTTTTTATTGATCAATATTCAACAAAAAGCAGTATTGAAAGATATTACACAGATTTAGTTAAGCTAAATAATTGA", "aa": "MKFLEYNFSLDIKDKKLIGVDEVGVGDYFGPLCCAAVFIPQDKIQEVIDLGVKDSKKLSDKQIKMMAYKLKKIVKYAIHHLSPKGYNTLNKTYNGNFLKMFTHLSAISSLQEKIGAVDYVFIDQYSTKSSIERYYTDLVKLNN", "aa_hexdigest": "90c5062305b19d17c4a57a36634ae7b4", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 176368, "stop": 177105, "observations": { "mutation": [ 176800 ], "stop": [ 176800 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE62", "query_cov": 1.0, "subject_cov": 0.572, "identity": 1.0, "score": 285.0, "evalue": 7.11e-96, "gene_start": 1, "gene_end": 143, "reference_start": 2, "reference_end": 144, "reference_sequence": "MMKFLEYNFSLDIKDKKLIGVDEVGVGDYFGPLCCAAVFIPQDKIQEVIDLGVKDSKKLSDKQIKMMAYKLKKIVKYAIHHLSPKGYNTLNKTYNGNFLKMFTHLSAISSLQEKIGAVDYVFIDQYSTKSSIERYYTDLVKLNNWADFNQIQGDILLAYKAESISLEVACASIIARDYFLYFMQKMNEQYNCKFPFGAGSKVKEFAREFFKNHPDSETKNNVCKKNFKMDLEDQNTTTINLFATDFENKK" }, "paralog": false, "identity": 0.9918367346938776, "score": 491.0, "evalue": 2.15e-179, "description": "Internal stop codon at 176800. Nonsense mutation around 176800.", "psc": { "uniref90_id": "UniRef90_C4XE62", "product": "Ribonuclease", "uniref50_id": "UniRef50_A0A6M4JBT7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE62", "UniRef:UniRef50_A0A6M4JBT7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6M4JBT7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6M4JBT7" ], "product": "Ribonuclease" } }, "id": "OAIKOHHJFB_348", "locus": "GCA000209735_00317" }, { "type": "cds", "sequence": "contig_1", "start": 176914, "stop": 177120, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAAATGAATGAGCAATATAATTGCAAATTTCCCTTTGGAGCAGGCTCAAAAGTCAAAGAATTTGCTCGTGAATTCTTCAAAAATCATCCTGACAGTGAAACAAAAAATAATGTTTGTAAAAAGAATTTTAAAATGGATTTAGAAGATCAAAACACAACAACTATTAATTTGTTTGCAACAGATTTTGAAAATAAAAAATAA", "aa": "MQKMNEQYNCKFPFGAGSKVKEFAREFFKNHPDSETKNNVCKKNFKMDLEDQNTTTINLFATDFENKK", "aa_hexdigest": "0bd711aaf46cf49880db4efe71bc2519", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8046.993199999998, "isoelectric_point": 8.599067115783694 }, "id": "OAIKOHHJFB_349", "locus": "GCA000209735_00318" }, { "type": "cds", "sequence": "contig_1", "start": 177390, "stop": 177998, "strand": "+", "frame": 3, "gene": "dnaK", "product": "Chaperone protein DnaK", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W7C0L0" ], "nt": "ATGGCAAAAGAAACTATTATTGGTATAGACTTAGGTACAACTAACTCAGCTGTAGCTATTGTTGATGGTGGTACACCAATCGTTCTTGAAAACTACAATGGTAAAAGAACAACTCCATCTGTTGTAAGTTTCAAAGATGGCGAAATTATTGTTGGTGAAAATGCCAAAAACCAAATCGAAACAAACCCAGATACTATTGCATCTGTAAAAAGATTCATGGGTACAAAAAAAATATTTAAAGCAAATGGAAAAGAATACAAACCAGAAGAAATTTCAGCTATTATTCTTGACCACTTAAGAAAATATGCAGAAGAAAAAGTTGGACACAAAATTGAAGCTGTTATTACAGTTCCTGCTTACTTTGACAATGCACAACGTGAAGCCACAAAAATCGCAGGTAAAATTGCAGGTTTAGACGTTCTTCGTATTATTAACGAACCTACTGCTGCTGCTTTAGCATTTGGACTTGATAAAACAAACAAAGAAATGAAAGTTCTTGTATTTGACCTTGGTGGTGGTACATTCGACGTTTCAATTCTTGAATTAGCTGATGGTACATTTGAAGTTCTTGCAACTTCAGGTGACAACAAACTTGGTGGTGATGACTGA", "aa": "MAKETIIGIDLGTTNSAVAIVDGGTPIVLENYNGKRTTPSVVSFKDGEIIVGENAKNQIETNPDTIASVKRFMGTKKIFKANGKEYKPEEISAIILDHLRKYAEEKVGHKIEAVITVPAYFDNAQREATKIAGKIAGLDVLRIINEPTAAALAFGLDKTNKEMKVLVFDLGGGTFDVSILELADGTFEVLATSGDNKLGGDD", "aa_hexdigest": "8bd49832f4183bd953212e299777438e", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_A0A7Y0YRM2", "query_cov": 0.9801980198019802, "subject_cov": 0.8181818181818182, "identity": 0.6970000000000001, "score": 266.0, "evalue": 2.32e-87, "valid": false, "product": "Chaperone protein DnaK", "uniref50_id": "UniRef50_W7C0L0" }, "pscc": { "uniref50_id": "UniRef50_W7C0L0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W7C0L0" ], "product": "Chaperone protein DnaK" }, "genes": [ "dnaK" ], "id": "OAIKOHHJFB_350", "locus": "GCA000209735_00319" }, { "type": "cds", "sequence": "contig_1", "start": 178071, "stop": 179144, "strand": "+", "frame": 3, "gene": "dnaK", "product": "molecular chaperone DnaK", "db_xrefs": [ "GO:0005524", "GO:0006457", "GO:0016887", "SO:0001217", "UniRef:UniRef50_Q49539", "UniRef:UniRef90_C4XE63" ], "nt": "ATGGCAATGGCTCGTCTAAAAGCTGCTGCTGAAAAAGCAAAAATTGACTTATCTTCATCATTAGTTGCTCACATTTCATTACCATTCCTTGTTTTATTAGACAATCATGAACCTATTAACGTTGAAGCTGAATTAAAACGTAGTGAATTCGAAAAAATGACTGCAAAACTTGTTGAACGTTGCCGTAGACCAATACAAGATGCTTTAAGTGAAGCTAAACTCAAGATTTCAGACTTAGATGAAATCTTACTTGTTGGTGGTTCAACACGTATTCCTGCTGTTCAAGCTCTTGTTGAAAAAATATTAAATAGAAAACCAAATAAATCAGTTAATCCTGATGAAGTTGTTGCAATGGGTGCTGCAATTCAAGGCGCTGTTCTTGCAGGTGACATTAACGACATTCTTTTAGTTGACGTTACACCTCTTACACTTGGTATTGAAACAGCTGGTGGTATCTCAACACCTCTTATTCCAAGAAACACACGTATTCCTATTACAAAGAGTGAAACATTTACAACATTTGAAAACAACCAAACAGATGTTACAATTAAAATTGTTCAAGGTGAAAGACCAGTTGCTTCAGAAAACAAATTATTAGGTCAATTTAACTTAACAGGTATTCGTCCAGCTCCTCGTGGAATTCCACAAATCGAAGTAAGTTTCAAAATTGATGCTAACGGTATAACAACAGTTTCTGCAAAAGATAAAGATACTCAAAAAGAACAAAGTATTACTATTAAAAACTCATCAAAACTTTCTGAAGAAGAAGTTGAAAGAATGATTAAAGAAGCTGAAGAAAACCGTGAAGCTGATGCAAAACGTGCTGCAGATATAGAAATTATTGTTCGTGCTGAAACAATGGTTGCTAAATTTGAAAGTGTTTTAGAAGAAAACAAAGACAAATTAACACAAGATCAAATTAATCAAGCTCAAGCTGAAATTGACAAAATCAATGGTTTTATCAAAGAAAAAGAATATGACCAACTTCGTTTAACAATCAAAGCTTTTGAAGAATTATTAGATTCAATGAGCAATGCAGACTCATCATCATTTAAAGAAGAAGATGCTGAATAG", "aa": "MAMARLKAAAEKAKIDLSSSLVAHISLPFLVLLDNHEPINVEAELKRSEFEKMTAKLVERCRRPIQDALSEAKLKISDLDEILLVGGSTRIPAVQALVEKILNRKPNKSVNPDEVVAMGAAIQGAVLAGDINDILLVDVTPLTLGIETAGGISTPLIPRNTRIPITKSETFTTFENNQTDVTIKIVQGERPVASENKLLGQFNLTGIRPAPRGIPQIEVSFKIDANGITTVSAKDKDTQKEQSITIKNSSKLSEEEVERMIKEAEENREADAKRAADIEIIVRAETMVAKFESVLEENKDKLTQDQINQAQAEIDKINGFIKEKEYDQLRLTIKAFEELLDSMSNADSSSFKEEDAE", "aa_hexdigest": "f932c8163746fec5f053c5d8492b0d77", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PNF8", "query_cov": 0.8991596638655462, "subject_cov": 0.9250720461095101, "identity": 0.579, "score": 346.0, "evalue": 4.94e-115, "valid": false, "product": "Chaperone protein DnaK", "uniref50_id": "UniRef50_A0A8S2D270" }, "genes": [ "dnaK" ], "truncated": "5-prime", "pseudogene": { "start": 177771, "stop": 179144, "observations": { "mutation": [ 177996, 178017 ], "stop": [ 177996, 178017 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE63", "query_cov": 1.0, "subject_cov": 0.6102564102564103, "identity": 1.0, "score": 645.0, "evalue": 2.81e-229, "gene_start": 1, "gene_end": 357, "reference_start": 229, "reference_end": 585, "reference_sequence": "MAKETIIGIDLGTTNSAVAIVDGGTPIVLENYNGKRTTPSVVSFKDGEIIVGENAKNQIETNPDTIASVKRFMGTKKIFKANGKEYKPEEISAIILDHLRKYAEEKVGHKIEKAVITVPAYFDNAQREATKIAGKIAGLDVLRIINEPTAAALAFGLDKTNKEMKVLVFDLGGGTFDVSILELADGTFEVLATSGDNKLGGDDWDHEIVDWLVAKIKNDHKIDIRENKMAMARLKAAAEKAKIDLSSSLVAHISLPFLVLLDNHEPINVEAELKRSEFEKMTAKLVERCRRPIQDALSEAKLKISDLDEILLVGGSTRIPAVQALVEKILNRKPNKSVNPDEVVAMGAAIQGAVLAGDINDILLVDVTPLTLGIETAGGISTPLIPRNTRIPITKSETFTTFENNQTDVTIKIVQGERPVASENKLLGQFNLTGIRPAPRGIPQIEVSFKIDANGITTVSAKDKDTQKEQSITIKNSSKLSEEEVERMIKEAEENREADAKRAADIEIIVRAETMVAKFESVLEENKDKLTQDQINQAQAEIDKINGFIKEKEYDQLRLTIKAFEELLDSMSNADSSSFKEEDAE" }, "paralog": false, "identity": 0.9956236323851203, "score": 861.0, "evalue": 1.12e-316, "description": "Internal stop codon at 177996, 178017. Nonsense mutation around 177996, 178017.", "psc": { "uniref90_id": "UniRef90_C4XE63", "gene": "dnaK", "product": "molecular chaperone DnaK", "uniref50_id": "UniRef50_Q49539", "go_ids": [ "GO:0005524", "GO:0006457", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE63", "UniRef:UniRef50_Q49539", "GO:0005524", "GO:0006457", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_Q49539", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49539" ], "product": "Chaperone protein DnaK" } }, "id": "OAIKOHHJFB_351", "locus": "GCA000209735_00320" }, { "type": "cds", "sequence": "contig_1", "start": 179333, "stop": 180010, "strand": "+", "frame": 2, "gene": null, "product": "AAA+ ATPase domain-containing protein", "db_xrefs": [ "KEGG:K07478", "SO:0001217", "UniParc:UPI0001A8C085", "UniRef:UniRef100_C4XE64", "UniRef:UniRef50_A0A449A4D0", "UniRef:UniRef90_C4XE64" ], "nt": "ATGAAAAATTTAGCTAATGAAATAAGACCAAGAACTTTAGATGACATTGTCGGCCAAAAGCATGTTGTCTCTTTATTAAAAGAAATAGTTAAAAATAAAGCCTCAACAAGCTTTATATTTTTTGGTGAAAGTGGAACTGGAAAAAGCTCAGCAGCAGTTGCTCTTGCTAATGACTTAAAACTTAAATATGGTTATTTTAATGCAACAATCAACAATAAAGATGAATTGGTCAATACCATTCAAACAAATGACATCATTATTATTGATGAAATTCACAGATTAAATAAAGACAAACAAGACATCTTACTTTCTTATTTAGAATATGACAAAATAATTGTTTATGCAACTACAACTGAGAATCCTTATTTTAAAGTCAATCCAGCTTTAAGAAGCAGAATGCAAATCTTGCAATTTAATAAACTTAGTGAAGAAGAAATTTTATTTGGACTTAAAAAAATCATCAAAACTTATTATCCAAAATTTAAAATCAAAGATGACATACTAAAAGTATTAATCAAAAATTCAACTGGCGATTATCGATCTTGTATTAACAATTTGCAAATTTTAACTTTACTTAATAAAGACAAAGAAGTCACTGAAGAAAGCATAAAAACTTTAATTCCAAATGTCAACTTTTATAGTGATAAAGAATCAAATGCTCACTACAACAACCTATAA", "aa": "MKNLANEIRPRTLDDIVGQKHVVSLLKEIVKNKASTSFIFFGESGTGKSSAAVALANDLKLKYGYFNATINNKDELVNTIQTNDIIIIDEIHRLNKDKQDILLSYLEYDKIIVYATTTENPYFKVNPALRSRMQILQFNKLSEEEILFGLKKIIKTYYPKFKIKDDILKVLIKNSTGDYRSCINNLQILTLLNKDKEVTEESIKTLIPNVNFYSDKESNAHYNNL", "aa_hexdigest": "a2c29c173beeaac2d41027341d4466c8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C085", "uniref100_id": "UniRef100_C4XE64", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C085", "UniRef:UniRef100_C4XE64" ] }, "ips": { "uniref100_id": "UniRef100_C4XE64", "uniref90_id": "UniRef90_C4XE64" }, "psc": { "uniref90_id": "UniRef90_C4XE64", "product": "AAA+ ATPase domain-containing protein", "uniref50_id": "UniRef50_A0A449A4D0", "kegg_orthology_id": "K07478" }, "pscc": { "uniref50_id": "UniRef50_A0A449A4D0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A4D0" ], "product": "ATPase" }, "genes": [], "id": "OAIKOHHJFB_352", "locus": "GCA000209735_00321" }, { "type": "cds", "sequence": "contig_1", "start": 180092, "stop": 180547, "strand": "+", "frame": 2, "gene": "mgsA", "product": "MgsA AAA+ ATPase C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE65", "UniRef:UniRef90_C4XE65" ], "nt": "ATGGGTCTTTTTAGAAGATTAACAGCAGCCACATATGAAGATGTAGGTTTAGCTGATCCAACATTACCAATGCGAGTAGAAGCTGCTTTTAATGCAGTGGAAAGATTAGGTTTTCCTGAAGCTTACTTACCTTTATATTACATAATAATTTCAGTAGCAATAGCTCCTAAAAGTAACACAGTTTATAAAGCAATTCAAAAAGTTGAACCTTTTATTGAGCAAGGTAACATTTATGAAATACCAAAACATTTAAGAGATGCACACTATAAATCAGCAAGCAAATTAGGAGATGGTCTTGATTATAAATATCCGCATGATTTTCCTAATCACTGGGTAAAACAGGAGTATTTGCCAAAAAGAATAGCAAGTAAAAAGTTTTTTGAACCAGATAATAATGATTCACCAAGCTTACTAAAATATTACAATTTTATAAAAAACATTAAAGGAGATAAATAA", "aa": "MGLFRRLTAATYEDVGLADPTLPMRVEAAFNAVERLGFPEAYLPLYYIIISVAIAPKSNTVYKAIQKVEPFIEQGNIYEIPKHLRDAHYKSASKLGDGLDYKYPHDFPNHWVKQEYLPKRIASKKFFEPDNNDSPSLLKYYNFIKNIKGDK", "aa_hexdigest": "8fffd4571d8719c4cc398c1fcbd391e9", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_C4XE65", "query_cov": 1.0, "subject_cov": 0.8728323699421965, "identity": 1.0, "score": 316.0, "evalue": 3.19e-109, "valid": true, "product": "MgsA AAA+ ATPase C-terminal domain-containing protein", "uniref50_id": "UniRef50_C4XE65" }, "pscc": { "uniref50_id": "UniRef50_C4XE65", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE65" ], "product": "MgsA AAA+ ATPase C-terminal domain-containing protein" }, "genes": [ "mgsA" ], "id": "OAIKOHHJFB_353", "locus": "GCA000209735_00322" }, { "type": "cds", "sequence": "contig_1", "start": 180975, "stop": 181196, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01409.25" ], "nt": "ATGCACGATTCATTATATTTAAATCCAACAACTCTTTTAAGAACACACAACACTGGAATTAGTGCACTTGAACTTGAAAGAAATGCAAACAAAGAAATGAATAATTTCGCTATTGGAAAAGTTTATCGTAATGATGAAGATGATGCAACTCACTCACATCAATTTACACAATTAGACTTTGTCAGTGTTGGCAAAAGTGTAAGCTTCAACAATTTAATTTGA", "aa": "MHDSLYLNPTTLLRTHNTGISALELERNANKEMNNFAIGKVYRNDEDDATHSHQFTQLDFVSVGKSVSFNNLI", "aa_hexdigest": "803e37801c05792fafefc81ae2c211d1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01409.25", "name": "tRNA-synt_2d", "length": 79, "aa_cov": 0.9863013698630136, "hmm_cov": 1.0, "evalue": 7.484430049807698e-21, "score": 70.78111267089844, "start": 1, "stop": 72 } ], "seq_stats": { "molecular_weight": 8295.0804, "isoelectric_point": 5.667268180847168 }, "id": "OAIKOHHJFB_354", "locus": "GCA000209735_00323" }, { "type": "cds", "sequence": "contig_1", "start": 181338, "stop": 181511, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01409.25" ], "nt": "ATGCTTCATCCAAATGTTATGGAACTTGCAGGTTATGATTCAAAAGAATTTAACGGTTTTGCTGCAGGCATTGGAATTGAACGTTTGACAATGATTAAATACAACATCAAGGATGTTAGAGAATTCTATAAAAATGACTTGAGAACTTTAAATCAATTTAACTATGAAGAATAG", "aa": "MLHPNVMELAGYDSKEFNGFAAGIGIERLTMIKYNIKDVREFYKNDLRTLNQFNYEE", "aa_hexdigest": "e6961cf0370b84c54345c6dea50dd53e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01409.25", "name": "tRNA-synt_2d", "length": 53, "aa_cov": 0.9298245614035088, "hmm_cov": 0.9811320754716981, "evalue": 1.4886412788312396e-19, "score": 66.52803802490234, "start": 1, "stop": 53 } ], "seq_stats": { "molecular_weight": 6748.5875, "isoelectric_point": 5.2735445022583 }, "id": "OAIKOHHJFB_355", "locus": "GCA000209735_00324" }, { "type": "cds", "sequence": "contig_1", "start": 181501, "stop": 181827, "strand": "+", "frame": 1, "gene": null, "product": "uracil-DNA glycosylase", "db_xrefs": [ "EC:3.2.2.27", "GO:0004844", "GO:0006284", "KEGG:K03648", "SO:0001217", "UniRef:UniRef50_N9V498", "UniRef:UniRef90_C4XE67" ], "nt": "ATGAAGAATAGCTTCCAAACATTTTTAAAACAAGAAGAATCTAAAAACTATTATCAAGAATTAATAAAAAAAGTTACTCAAGAAGAACAAAAACACAATGTTTTTCCACCTAAAGAATTACGTTTTGATGCTTTAAATTATTTTGAAGCAGAAGAGACTAAATTAATAATAATTGGACAAGATCCTTATTATTTAAAAAACCAAGCAGATGGTTTAGCTTTTAGTACTCAAGGCAATATTTGTCCTAAAAGTTTAAACAATATTTTTAAAGAATTAAAGAAAGATTATTCTGAAACAAACATTGAAACTTATAGCTTAAAAAGTTGA", "aa": "MKNSFQTFLKQEESKNYYQELIKKVTQEEQKHNVFPPKELRFDALNYFEAEETKLIIIGQDPYYLKNQADGLAFSTQGNICPKSLNNIFKELKKDYSETNIETYSLKS", "aa_hexdigest": "37aceefcce60e4e6c6652ad0d75aad80", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 181489, "stop": 182130, "observations": { "mutation": [ 181825, 181906, 181975 ], "stop": [ 181825, 181906, 181975 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE67", "query_cov": 1.0, "subject_cov": 0.47577092511013214, "identity": 1.0, "score": 214.0, "evalue": 7.71e-69, "gene_start": 1, "gene_end": 108, "reference_start": 5, "reference_end": 112, "reference_sequence": "MNLTMKNSFQTFLKQEESKNYYQELIKKVTQEEQKHNVFPPKELRFDALNYFEAEETKLIIIGQDPYYLKNQADGLAFSTQGNICPKSLNNIFKELKKDYSETNIETYSLKSWAKQGVLLINTCLSVNENQPLSHKNFGWEIFVINLIKYILKKNKNVIFGIWGNDAKRILQIVQNDYKIDENKIIFTSHPSPLAYSKTTNSFKDSKFFIRVNSLLKNPINWDLRKD" }, "paralog": false, "identity": 0.9812206572769953, "score": 416.0, "evalue": 1.19e-150, "description": "Internal stop codon at 181825, 181906, 181975. Nonsense mutation around 181825, 181906, 181975.", "psc": { "uniref90_id": "UniRef90_C4XE67", "product": "uracil-DNA glycosylase", "ec_ids": [ "3.2.2.27" ], "uniref50_id": "UniRef50_N9V498", "kegg_orthology_id": "K03648", "go_ids": [ "GO:0004844", "GO:0006284" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE67", "EC:3.2.2.27", "UniRef:UniRef50_N9V498", "KEGG:K03648", "GO:0004844", "GO:0006284" ] }, "pscc": { "uniref50_id": "UniRef50_N9V498", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9V498" ], "product": "Uracil-DNA glycosylase" } }, "id": "OAIKOHHJFB_356", "locus": "GCA000209735_00325" }, { "type": "cds", "sequence": "contig_1", "start": 182174, "stop": 182752, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01588.26" ], "nt": "ATGATTATTTCATTAAGAGAATTAAATAAATTTATGCCTAAAATTAAGTTAGATGAAACTATTGAAAAGGTCATTAATAATTTAGGTTATGAAGTTGAAAGCATGACTAAATTTAGTGATGTTAAAGGAATTAAGTTTGCTAGAATAATTGATGTTAAACCTAATCCAAAATCAAATGGTTTAACAATTGTTGAAGTTGAAACTGATAAAGGAAAATTAACAATTCAAACCAAAGCACAAAATGCGCAGAAGAACAAAATAGCTGTTATTTTTGTTAAAGGAAGTCAAAAAGGCGACTTTGTATTTCAAGATAAAGAAATGGCAGGCGTTGTTTCTCAAGGTATGTTTTCAGGTTATAGTGAGTTAGGTTATGATCCAAATAAATTGCCTTTTGATAAAGATGATATTATTTTGTTAAATCCTAATAGATATACAAGTTTAAAAACTGATCCTATGGAATATTTTGGCTTAAATGACTATATTATTGATATTACCACTCCAGCAAATAGAGGCGATTCAAATAGTTATTATGTTTTAGCTCAAGAAATAGCAGCCTACTATCAAACTCCTTTTAAATGA", "aa": "MIISLRELNKFMPKIKLDETIEKVINNLGYEVESMTKFSDVKGIKFARIIDVKPNPKSNGLTIVEVETDKGKLTIQTKAQNAQKNKIAVIFVKGSQKGDFVFQDKEMAGVVSQGMFSGYSELGYDPNKLPFDKDDIILLNPNRYTSLKTDPMEYFGLNDYIIDITTPANRGDSNSYYVLAQEIAAYYQTPFK", "aa_hexdigest": "0ebbddf26e49b7727938c6bf77eb5f09", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01588.26", "name": "tRNA_bind", "length": 84, "aa_cov": 0.40625, "hmm_cov": 0.9047619047619048, "evalue": 4.117160217165083e-08, "score": 29.353538513183594, "start": 44, "stop": 121 } ], "seq_stats": { "molecular_weight": 21730.671700000006, "isoelectric_point": 6.372071647644043 }, "id": "OAIKOHHJFB_357", "locus": "GCA000209735_00326" }, { "type": "cds", "sequence": "contig_1", "start": 183119, "stop": 184051, "strand": "+", "frame": 2, "gene": null, "product": "phenylalanine--tRNA ligase subunit beta", "db_xrefs": [ "EC:6.1.1.20", "KEGG:K01890", "SO:0001217", "UniRef:UniRef50_A0A3P8L797", "UniRef:UniRef90_C4XE68" ], "nt": "ATGGGCTTAGAAGAAACTGCAGCAAATCGTAAAAGTAAATATGTTGTTTTTGAAATTGGTAATTTTAATCCTTCTGATGTTAGACATACAGCTAAAGAAATTAAAATTGATTCAGCTTCATCATTGCAAGGCGGTAAGGTTTTAAGTACAGAATTAGTAAGAAATGGTATGGAATATTTAAAATATAAAGCTTTTGAAGATAAGCAACCTTCTTCAGTTTTAATTAATTTGCCATCAAAGACTAAGAATAAAATTTTCTTACAACAAAAGAAATTATTAGGAAAATACTCAGGATTGCAAATCAAGGATTTGGACAAAGAATTTGAAAAAGTTGAGGATCAATTGCAAAGAATTGGATTCAAAATATTTTATAATCGTCTTTCAGCTCCAACTTATAGAACTGATTTAAATTGTTATGAAGACATAATTGAAGAATACTTTAGATTCTATGGTTATGACAAATTTAAAACTGAAGCTCCTGCATTAGCTCCATTTAAAATTAACAAAAGGAATAATGATAAAAGCTTATTACAATCAATGGGTTATCAAGAAGTAAGAACATTTACTTTAACAAATATTGATGCTCTTAAATTTAATCCTTTTGAATTTAAAGAAGATTTAAAACTTCAAACTTTTGTTTCAAAAGAAAGAGAAGCAATTAGAAATTCAATTGTTCCTTCACTTTTAGAAGTTGCAGAATACAACCTAAAAAGAAAAATGAATCGTTTAAATTTATTTGAATGTGGAATGATTAATAATGGAAAATATGTTTACGGTTTACTATCAAATACAAAAACTTTTGAACAAGTAAAACAAGATGTAGTTAACTTTTTAAATGATAATAATTTAGTATTTAAACCTTTTAAAGATAATCAATTTATACATCCAAATGTATCAGCAAAAATTTACCAAAACAACAGTTTTATAGGTTGA", "aa": "MGLEETAANRKSKYVVFEIGNFNPSDVRHTAKEIKIDSASSLQGGKVLSTELVRNGMEYLKYKAFEDKQPSSVLINLPSKTKNKIFLQQKKLLGKYSGLQIKDLDKEFEKVEDQLQRIGFKIFYNRLSAPTYRTDLNCYEDIIEEYFRFYGYDKFKTEAPALAPFKINKRNNDKSLLQSMGYQEVRTFTLTNIDALKFNPFEFKEDLKLQTFVSKEREAIRNSIVPSLLEVAEYNLKRKMNRLNLFECGMINNGKYVYGLLSNTKTFEQVKQDVVNFLNDNNLVFKPFKDNQFIHPNVSAKIYQNNSFIG", "aa_hexdigest": "24897d433d68fab6222ca4e15ece78af", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 182819, "stop": 184354, "observations": { "mutation": [ 182921, 184049, 184091 ], "stop": [ 182921, 184049, 184091 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE68", "query_cov": 1.0, "subject_cov": 0.4246575342465753, "identity": 1.0, "score": 612.0, "evalue": 4.72e-215, "gene_start": 1, "gene_end": 310, "reference_start": 318, "reference_end": 627, "reference_sequence": "MNMIISLRELNKFMPKIKLDETIEKVINNLGYEVESMTKFSDVKGIKFARIIDVKPNPKSNGLTIVEVETDKGKLTIQTKAQNAQKNKIAVIFVKGSQKGDFVFQDKEMAGVVSQGMFSGYSELGYDPNKLPFDKDDIILLNPNRYTSLKTDPMEYFGLNDYIIDITTPANRGDSNSYYVLAQEIAAYYQTPFKWFDYKKNYKTGIHKQIQKVDNGEANALSFLECRFKHKDTKIEDMLFLAKHNVEAKNVWAIDVTNLNLIYTGAPTHVYDRSEIGEELTCKKYSGKVTILGNKEIEVKNALAIYSKDKVVSLASVMGLEETAANRKSKYVVFEIGNFNPSDVRHTAKEIKIDSASSLQGGKVLSTELVRNGMEYLKYKAFEDKQPSSVLINLPSKTKNKIFLQQKKLLGKYSGLQIKDLDKEFEKVEDQLQRIGFKIFYNRLSAPTYRTDLNCYEDIIEEYFRFYGYDKFKTEAPALAPFKINKRNNDKSLLQSMGYQEVRTFTLTNIDALKFNPFEFKEDLKLQTFVSKEREAIRNSIVPSLLEVAEYNLKRKMNRLNLFECGMINNGKYVYGLLSNTKTFEQVKQDVVNFLNDNNLVFKPFKDNQFIHPNVSAKIYQNNSFIGWLGKVNPSYNSVDAWVCEVHKEVNKGYKLFENYDSTPFKSIDLTFELKPHQSIDDKIIEIKKVAFIFEIYQIDEFAKPESRNVTLRIIGSNEEIQKINNEFNN" }, "paralog": false, "identity": 0.9941291585127201, "score": 1004.0, "evalue": 0.0, "description": "Internal stop codon at 182921, 184049, 184091. Nonsense mutation around 182921, 184049, 184091.", "psc": { "uniref90_id": "UniRef90_C4XE68", "product": "phenylalanine--tRNA ligase subunit beta", "ec_ids": [ "6.1.1.20" ], "uniref50_id": "UniRef50_A0A3P8L797", "kegg_orthology_id": "K01890", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE68", "EC:6.1.1.20", "UniRef:UniRef50_A0A3P8L797", "KEGG:K01890" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8L797", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8L797" ], "product": "Phenylalanine--tRNA ligase beta subunit" } }, "id": "OAIKOHHJFB_358", "locus": "GCA000209735_00327" }, { "type": "cds", "sequence": "contig_1", "start": 184369, "stop": 184908, "strand": "+", "frame": 1, "gene": null, "product": "Serine hydroxymethyltransferase", "db_xrefs": [ "EC:2.1.2.1", "KEGG:K00600", "SO:0001217", "UniRef:UniRef50_P56089", "UniRef:UniRef90_C4XE69" ], "nt": "ATGTATAACAAAATTAAATTATTGGACAAAAAAATTGAAGAAGCAATTAACAATGAAACTGATCGCCAAAATAATCATATTGAATTAATTGCATCAGAAAACTATGTTTCAGAAGATGTTTTAAAAGCAACAGGAAGCATTCTAACAAATAAATATGGTGAAGGTTATCCTTACCGTAGATATTATGGTGGCTGCGAAAATGTTGATGTTGTTGAAAATTTAGCAATCGAAAGAGCCAAAAAATTATTTAATGTTAAATTTGCTAATGTTCAACCTTATTCAGGCAGTGTTGCTAATGCAGCAGCCTTAGCTTCTTTAGCAAAAACAGGCGAAAAAATTATGGGGCTTTCACTTGCATCGGGTGGTCACTTAACTCATGGTTACAAGATTTCATTTAGTGGAATTTTATATAATAGCGTTTCATATGAAGTAGATAAAAATGGATTCTTAGATTATGAAGCAATTAAAAAAATAGCTATTAAAGAAAAACCAAAAGTAATTATTGCTGGTTACTCAGCTTATTCAAGAATTATTGATTGA", "aa": "MYNKIKLLDKKIEEAINNETDRQNNHIELIASENYVSEDVLKATGSILTNKYGEGYPYRRYYGGCENVDVVENLAIERAKKLFNVKFANVQPYSGSVANAAALASLAKTGEKIMGLSLASGGHLTHGYKISFSGILYNSVSYEVDKNGFLDYEAIKKIAIKEKPKVIIAGYSAYSRIID", "aa_hexdigest": "d9f4a5bd7785b51daa98e8967244b4ad", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI002618815A", "query_cov": 0.9776536312849162, "subject_cov": 0.8293838862559242, "identity": 0.5770000000000001, "score": 197.0, "evalue": 3.74e-61, "valid": false, "product": "serine hydroxymethyltransferase", "ec_ids": [ "2.1.2.1" ], "uniref50_id": "UniRef50_A0A533YJM1", "kegg_orthology_id": "K00600" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 184357, "stop": 185211, "observations": { "mutation": [ 184906, 185116 ], "stop": [ 184906, 185116 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE69", "query_cov": 1.0, "subject_cov": 0.4221698113207547, "identity": 1.0, "score": 345.0, "evalue": 1.87e-116, "gene_start": 1, "gene_end": 179, "reference_start": 5, "reference_end": 183, "reference_sequence": "MGGIMYNKIKLLDKKIEEAINNETDRQNNHIELIASENYVSEDVLKATGSILTNKYGEGYPYRRYYGGCENVDVVENLAIERAKKLFNVKFANVQPYSGSVANAAALASLAKTGEKIMGLSLASGGHLTHGYKISFSGILYNSVSYEVDKNGFLDYEAIKKIAIKEKPKVIIAGYSAYSRIIDWAKFREIADACGAYLMADIAHISGLIIAGVHPSPVGYADVITTTTHKTLRGARGAIIMTNNEELAKKIDRWVFPGYQGGPLFHTIAGKAVAFGEALLPSFKEYGKKIVENSKEFAQAFLDKNVAIVSGGTDNHLFTINVYQSYKISGKDAESLLNKFNITVNKNTVPFDTLSPMIASGIRLGTAAMTSRKFTKWRELADIIDTILKNHETILKPESEKLFNSLKKKVRDFTKKFPIITVYR" }, "paralog": false, "identity": 0.9894366197183099, "score": 554.0, "evalue": 5.3e-201, "description": "Internal stop codon at 184906, 185116. Nonsense mutation around 184906, 185116.", "psc": { "uniref90_id": "UniRef90_C4XE69", "product": "Serine hydroxymethyltransferase", "ec_ids": [ "2.1.2.1" ], "uniref50_id": "UniRef50_P56089", "kegg_orthology_id": "K00600", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE69", "EC:2.1.2.1", "UniRef:UniRef50_P56089", "KEGG:K00600" ] }, "pscc": { "uniref50_id": "UniRef50_P56089", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P56089" ], "product": "Serine hydroxymethyltransferase" } }, "id": "OAIKOHHJFB_359", "locus": "GCA000209735_00328" }, { "type": "cds", "sequence": "contig_1", "start": 184951, "stop": 185118, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00464.25" ], "nt": "ATGGCTGATATTGCGCATATTTCAGGTTTAATTATTGCAGGTGTACATCCTAGTCCAGTAGGCTATGCTGATGTTATTACCACTACAACTCATAAAACTTTAAGAGGTGCTAGAGGTGCAATTATAATGACTAACAATGAAGAGTTAGCTAAGAAAATTGATCGCTGA", "aa": "MADIAHISGLIIAGVHPSPVGYADVITTTTHKTLRGARGAIIMTNNEELAKKIDR", "aa_hexdigest": "345e31943a1700467e5aca3128ae9edf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00464.25", "name": "SHMT", "length": 46, "aa_cov": 0.8363636363636363, "hmm_cov": 1.0, "evalue": 2.3854474460262562e-22, "score": 75.16732025146484, "start": 1, "stop": 46 } ], "seq_stats": { "molecular_weight": 5839.704599999999, "isoelectric_point": 8.247972297668458 }, "id": "OAIKOHHJFB_360", "locus": "GCA000209735_00329" }, { "type": "cds", "sequence": "contig_1", "start": 185173, "stop": 185487, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00464.25" ], "nt": "GTGGCTTTTGGAGAAGCGTTATTGCCTTCATTTAAAGAATATGGTAAAAAAATAGTAGAAAATTCTAAAGAATTTGCACAAGCATTTTTAGATAAAAACGTTGCTATTGTTTCTGGTGGAACTGATAACCATTTATTTACAATCAATGTATATCAAAGCTATAAAATTTCAGGTAAAGATGCTGAAAGTTTGTTAAATAAATTTAACATAACAGTTAACAAAAATACAGTGCCTTTTGACACTTTAAGTCCTATGATTGCGAGCGGTATTAGATTAGGCACAGCAGCTATGACTTCAAGAAAATTTACTAAATGA", "aa": "MAFGEALLPSFKEYGKKIVENSKEFAQAFLDKNVAIVSGGTDNHLFTINVYQSYKISGKDAESLLNKFNITVNKNTVPFDTLSPMIASGIRLGTAAMTSRKFTK", "aa_hexdigest": "11dd0db7be7e1ddd133574db06e7805b", "start_type": "GTG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00464.25", "name": "SHMT", "length": 102, "aa_cov": 0.9807692307692307, "hmm_cov": 0.9803921568627451, "evalue": 2.7885565361532164e-34, "score": 114.4564437866211, "start": 2, "stop": 103 } ], "seq_stats": { "molecular_weight": 11428.000799999985, "isoelectric_point": 9.435479927062985 }, "id": "OAIKOHHJFB_361", "locus": "GCA000209735_00330" }, { "type": "cds", "sequence": "contig_1", "start": 185907, "stop": 186164, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCTGCAACCATTATTCAGTTAGCAATATATTTGCTTCCAAATTTGTTGAAAATACCAATGGCAATACCAAACATACTTGGCATAATTACAGCAAAAGTTATAAAGTTTTCCATAATTGAAGCCATTATTGATTTACCAATATCATTTGTTCCTTGGAAAACAAGAAGTGAGCTAATTGATAAACTCAAAGCAATAACTCTTAGTGTAACTATTCTAATAAATAAAGTAGTTCCAGCAGCTCTATCTGGTTGA", "aa": "MNAATIIQLAIYLLPNLLKIPMAIPNILGIITAKVIKFSIIEAIIDLPISFVPWKTRSELIDKLKAITLSVTILINKVVPAALSG", "aa_hexdigest": "38101b8e94f8a3d0d2697a829e0a3953", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9218.25609999999, "isoelectric_point": 9.777162361145017 }, "id": "OAIKOHHJFB_362", "locus": "GCA000209735_00331" }, { "type": "cds", "sequence": "contig_1", "start": 186254, "stop": 186514, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTGCCTACTTTTGCCTTAGGTTTTAGTGGTTTCATAAGATCATTTGGTGTTGCTATTTCATTTGCTGTTATGAACATTTTCTTAACCAAAACTCCTTTTGCGGAACCACTTTCATTCCAATATTATTGGTCAAAAGGTATGCCGATTGTTTCATTATTCTTAGTTTCTATTTTTGGTATTAATGATGGAGCTAGAAGCTTAATGTCATATAACTATACGCGAAGAAACTTTAAAAGATGCAAAGAAGTTTATCTTTGA", "aa": "MLPTFALGFSGFIRSFGVAISFAVMNIFLTKTPFAEPLSFQYYWSKGMPIVSLFLVSIFGINDGARSLMSYNYTRRNFKRCKEVYL", "aa_hexdigest": "9d5d739d0015717808804b47359bbafe", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9872.533399999991, "isoelectric_point": 9.916414070129392 }, "id": "OAIKOHHJFB_363", "locus": "GCA000209735_00332" }, { "type": "cds", "sequence": "contig_1", "start": 186560, "stop": 186664, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGTATTAGGTGGTGTTGTTGCTACTATTATTGGTTGGTCAATTAACTTAACTGCCTACTTAGCTTATGTGGTAATTAATAGCAAGCGGAATAAAACCTGA", "aa": "MGVLGGVVATIIGWSINLTAYLAYVVINSKRNKT", "aa_hexdigest": "3d1d62a2394cb888ef13294ae4f8eb2c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3623.2691000000004, "isoelectric_point": 9.996161460876465 }, "id": "OAIKOHHJFB_364", "locus": "GCA000209735_00333" }, { "type": "cds", "sequence": "contig_1", "start": 187028, "stop": 187294, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTCAATTTTTACCAGATACTTTAAACTCTTTAACTTCAAACGAATTGAAGCAGAAATACTTGGATATCATAAATAACTCCAGTTATAAAGATCTGAATTTAGGAAAAATTGATGCTAATGGGGTTGTTTCAATGACCACAGCTGCAATTCAACCTTTAATTATTTTTTCTAACTCAATAGTATTTTTAGTACCTGTTGGTGCAAGTATTTATTACACAAAATGTATTTCTAAAAAATTAGAAAAAAGTGGAAGAGACTTGTGA", "aa": "MIQFLPDTLNSLTSNELKQKYLDIINNSSYKDLNLGKIDANGVVSMTTAAIQPLIIFSNSIVFLVPVGASIYYTKCISKKLEKSGRDL", "aa_hexdigest": "203bcc40dc06ae7e616f1f5464935e5e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9728.218799999995, "isoelectric_point": 8.759271049499514 }, "id": "OAIKOHHJFB_365", "locus": "GCA000209735_00334" }, { "type": "tRNA", "sequence": "contig_1", "start": 187576, "stop": 187651, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(ggt)", "amino_acid": "Thr", "anti_codon": "ggt", "score": 75.0, "nt": "GCCGTCATAGCTCAGTCGGTAGAGCACATCCATGGTAAGGATGGGGTCGCTGGTTCAAGTCCAGTTGTCGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 187632, 187634 ], "id": "OAIKOHHJFB_25", "locus": "GCA000209735_00335" }, { "type": "cds", "sequence": "contig_1", "start": 187901, "stop": 189022, "strand": "+", "frame": 2, "gene": "dnaJ", "product": "Chaperone protein DnaJ", "db_xrefs": [ "RefSeq:WP_013526678.1", "SO:0001217", "UniParc:UPI0001A8C08C", "UniRef:UniRef100_C4XE71", "UniRef:UniRef50_C4XE71", "UniRef:UniRef90_C4XE71" ], "nt": "ATGCCTAAAAAGAATTATTATGATGTGCTAGGTGTTCCAAAAACAGCTAGTGAAAGAGAAATTAAAACTGCTTACCGTAAATTAGCAATGAAATATCACCCTGATAAATTAAAAGATGGTACCAGTGATCAAAAGATGCAAGAGCTAAATGAAGCTTATGAAGTTTTAAGTGATCCAACCAAAAAATCTAATTATGATCGTTTTGGTTCACCAGATGGTCCACGACCTGGACAAGGTTTTGGAATGAACTTTGGTGGAGAAATGAGAGACTTTAGCAAGTTTACTCAAAATATTTTTGATACAGTATTCAATTTTGGAGGTGGAGCTTCAAAACAAAAATCTTCAAAAGTAGAAAAAGCAAGAGGCGATGATATTTACACTGAAATTGTAGTTGATTTCATTGAAGCTGTTAAAGGTAAAGAGTTTAAAGAAAAATTAAATAAATTTGAATTATGTAGTAGATGTAAAGGAACAGGAGCTGATAATCCAAGTGATGTTAGAACTTGTAATGTTTGTCAAGGAAAGGGACAAAAAGAAACACAAACACGTTCTGTACTAGGTTACATGAAAATGGTTTCAACATGTGATAAATGTAATGGAACAGGTAAATTAATTGGCAAAACTTGTCGTGAATGTAATGGATCTTTATATACGAAAAAATTAAAAAATGTTACTTTCAAAATTCCGGAAGGTTCAGACAGCGGCGACAAAATTAAAATCGATGGTTATGGCGAAAAAGGTCGTAATGGTGGTGAATCAGGTGACTTATACATTGTTATCATTGTTAAAGAACATAAATACTTCAAGAGAAATGGTCTAGATTTATTCTTAGAATTGCCAGTTTCTTTCTTAGATATTGTTAAAGAAAATTCAGTTTTAGTTCCAACACCATACGGATTTGAAAGAATTAAAATGAAAAATACTTACCAATTTGGTAAAGTTCTTAATTTAACAGGAAAAGGGATTAGAACTAAAAAAGGAACGGGAAATCTAAAAGTAGCTCTTCAAGTTGTTATGCCTAATTTCACAAGTGACCAATATAATGAAATGGCAAAAATTTTAGAACCATATGAAGATAATGTTAATGCAGAATTTTCAGAATTAGTTAAAAGCGAAAAATAA", "aa": "MPKKNYYDVLGVPKTASEREIKTAYRKLAMKYHPDKLKDGTSDQKMQELNEAYEVLSDPTKKSNYDRFGSPDGPRPGQGFGMNFGGEMRDFSKFTQNIFDTVFNFGGGASKQKSSKVEKARGDDIYTEIVVDFIEAVKGKEFKEKLNKFELCSRCKGTGADNPSDVRTCNVCQGKGQKETQTRSVLGYMKMVSTCDKCNGTGKLIGKTCRECNGSLYTKKLKNVTFKIPEGSDSGDKIKIDGYGEKGRNGGESGDLYIVIIVKEHKYFKRNGLDLFLELPVSFLDIVKENSVLVPTPYGFERIKMKNTYQFGKVLNLTGKGIRTKKGTGNLKVALQVVMPNFTSDQYNEMAKILEPYEDNVNAEFSELVKSEK", "aa_hexdigest": "2808ef31d4a480422d963c1a69d9a2ce", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C08C", "ncbi_nrp_id": "WP_013526678.1", "uniref100_id": "UniRef100_C4XE71", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C08C", "RefSeq:WP_013526678.1", "UniRef:UniRef100_C4XE71" ] }, "ips": { "uniref100_id": "UniRef100_C4XE71", "uniref90_id": "UniRef90_C4XE71" }, "psc": { "uniref90_id": "UniRef90_C4XE71", "product": "Chaperone protein DnaJ", "uniref50_id": "UniRef50_C4XE71" }, "pscc": { "uniref50_id": "UniRef50_C4XE71", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE71" ], "product": "Chaperone protein DnaJ" }, "genes": [ "dnaJ" ], "id": "OAIKOHHJFB_366", "locus": "GCA000209735_00336" }, { "type": "cds", "sequence": "contig_1", "start": 189059, "stop": 189295, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE72", "UniRef:UniRef90_C4XE72" ], "nt": "ATGAAAAATGACAAATTAGTTCAAGAAAAATTAAACCAAATTAATGGCACATATGATGAAAATTCAATGTTTAAAAGTTTAATTAATAGCGATGAAGAATTGCTCAAAAATTTTAAATTGTTTTGTGAAATTTATCAAAACAACAATTTTCTTGAACAAAAATTTTTAAACCATACAGTCAATGAATTTAAGAGAAATAAAAATGAAGAAATTGATTTATTTGACTTAAAAAAATGA", "aa": "MKNDKLVQEKLNQINGTYDENSMFKSLINSDEELLKNFKLFCEIYQNNNFLEQKFLNHTVNEFKRNKNEEIDLFDLKK", "aa_hexdigest": "7eb248a538f98237d26455d7d21a1f52", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 189056, "stop": 189535, "observations": { "mutation": [ 189293 ], "stop": [ 189293 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE72", "query_cov": 1.0, "subject_cov": 0.49056603773584906, "identity": 1.0, "score": 152.0, "evalue": 8.01e-46, "gene_start": 1, "gene_end": 78, "reference_start": 2, "reference_end": 79, "reference_sequence": "MMKNDKLVQEKLNQINGTYDENSMFKSLINSDEELLKNFKLFCEIYQNNNFLEQKFLNHTVNEFKRNKNEEIDLFDLKKWIDLKNKVDSYDTQNFDLSNNFFKKMSLKSGELSFLYDSFINENDEFGEFDFGEESRFNNNETSVIAKTTEVVVNKVLKL" }, "paralog": false, "identity": 0.9874213836477987, "score": 315.0, "evalue": 1.29e-112, "description": "Internal stop codon at 189293. Nonsense mutation around 189293.", "psc": { "uniref90_id": "UniRef90_C4XE72", "uniref50_id": "UniRef50_C4XE72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE72", "UniRef:UniRef50_C4XE72" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE72" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9479.60649999999, "isoelectric_point": 5.366135215759278 }, "id": "OAIKOHHJFB_367", "locus": "GCA000209735_00337" }, { "type": "cds", "sequence": "contig_1", "start": 189368, "stop": 189535, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTTTAAAATCAGGAGAATTGTCATTTCTTTATGATTCATTTATTAATGAAAATGATGAATTTGGCGAATTTGATTTTGGAGAGGAATCAAGATTCAATAATAATGAAACTTCAGTCATTGCAAAAACAACAGAAGTTGTAGTTAACAAAGTTTTAAAACTATAA", "aa": "MSLKSGELSFLYDSFINENDEFGEFDFGEESRFNNNETSVIAKTTEVVVNKVLKL", "aa_hexdigest": "4d58b6faf28baa5f09a76ea62af54572", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6282.857500000002, "isoelectric_point": 4.286308097839356 }, "id": "OAIKOHHJFB_368", "locus": "GCA000209735_00338" }, { "type": "cds", "sequence": "contig_1", "start": 189654, "stop": 189800, "strand": "+", "frame": 3, "gene": null, "product": "4'-phosphopantetheinyl transferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E5U8", "UniRef:UniRef90_C4XE73" ], "nt": "ATGATAGGTGTTGATTTAGTTAAAATTTCCAGATTTGAAAAAGCAAAAATTGATTTTGTTAAAAAGTTTTTACATCCAAACGAACTGGAAAAATATTTGCTGATAAAAGACAATTATTTGAAAAGTAAATTTTTAGCAGCAATTTGA", "aa": "MIGVDLVKISRFEKAKIDFVKKFLHPNELEKYLLIKDNYLKSKFLAAI", "aa_hexdigest": "d87b1d34bf5650a801bdbba8e7e3782f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 189642, "stop": 189962, "observations": { "mutation": [ 189798, 189882 ], "stop": [ 189798, 189882 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE73", "query_cov": 1.0, "subject_cov": 0.4528301886792453, "identity": 1.0, "score": 95.9, "evalue": 1.1e-24, "gene_start": 1, "gene_end": 48, "reference_start": 5, "reference_end": 52, "reference_sequence": "MIRTMIGVDLVKISRFEKAKIDFVKKFLHPNELEKYLLIKDNYLKSKFLAAIWAIKEAMFKVDNSLFEFKNIKLQKENESWQHKNFDISISHEDDYLVAVVLKKKE" }, "paralog": false, "identity": 0.9716981132075472, "score": 199.0, "evalue": 4.16e-68, "description": "Internal stop codon at 189798, 189882. Nonsense mutation around 189798, 189882.", "psc": { "uniref90_id": "UniRef90_C4XE73", "product": "4'-phosphopantetheinyl transferase domain-containing protein", "uniref50_id": "UniRef50_D5E5U8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE73", "UniRef:UniRef50_D5E5U8" ] }, "pscc": { "uniref50_id": "UniRef50_D5E5U8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E5U8" ], "product": "Holo-[acyl-carrier-protein] synthase" } }, "id": "OAIKOHHJFB_369", "locus": "GCA000209735_00339" }, { "type": "cds", "sequence": "contig_1", "start": 190183, "stop": 190581, "strand": "+", "frame": 1, "gene": null, "product": "Phospholipid/glycerol acyltransferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE74", "UniRef:UniRef90_C4XE74" ], "nt": "TTGATTCCAAACCACAAATCAAATGCTGATGCTTTGATCTTAATGGAAGCTTTATCTAAGAAAACCGCATCAAAAGAAGAAATGAACAAAATAGCAACATTTTTAGCTAAAAAAGAATTATCTAAAAAAGCTGTTGTTAAAAGTGCACTTAATTTAATTGACTCAATTTACATTGATAGAAATAACTTTAGAGATGCTTTTGAAAGCTTAACTAAATTTGGAACTTTTATTAAAGAAAATAGAACTTATGGTGTTATCTTTCCTGAAGGTACAAGAGTTGAAGAAGATGGCTTAGGTGAATTTAAATCAGGTGCTTTCAAAGTTGCAATCAGTCATTATTTACCAATAGTACCTGTAGTTATTAGTGACACAAGAAAAGCACTTAACAAAAACAGATGA", "aa": "MIPNHKSNADALILMEALSKKTASKEEMNKIATFLAKKELSKKAVVKSALNLIDSIYIDRNNFRDAFESLTKFGTFIKENRTYGVIFPEGTRVEEDGLGEFKSGAFKVAISHYLPIVPVVISDTRKALNKNR", "aa_hexdigest": "23c9f92da5bd43a2c14388764de830ea", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 189937, "stop": 190710, "observations": { "mutation": [ 189946, 189997, 190003, 190009, 190090, 190579 ], "stop": [ 189946, 189997, 190003, 190009, 190090, 190579 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE74", "query_cov": 1.0, "subject_cov": 0.5136186770428015, "identity": 0.992, "score": 254.0, "evalue": 1.2e-83, "gene_start": 1, "gene_end": 132, "reference_start": 83, "reference_end": 214, "reference_sequence": "MWFWKRRSNMNYHLKMFLTWWIWLWRIYRISVYARKYRHTPDYYTPQQRYDWLLKKVKKYLKLYNIKVTVEGYDNLPKAPCLLIPNHKSNADALILMEALSKKTASKEEMNKIATFLAKKELSKKAVVKSALNLIDSIYIDRNNFRDAFESLTKFGTFIKENRTYGVIFPEGTRVEEDGLGEFKSGAFKVAISHYLPIVPVVISDTRKALNKNRWKKQEFKVKFLNPIKPSTFMSMDPGALADRVKSLMERELENES" }, "paralog": false, "identity": 0.9727626459143969, "score": 491.0, "evalue": 1.28e-179, "description": "Internal stop codon at 189946, 189997, 190003, 190009, 190090, 190579. Nonsense mutation around 189946, 189997, 190003, 190009, 190090, 190579.", "psc": { "uniref90_id": "UniRef90_C4XE74", "product": "Phospholipid/glycerol acyltransferase domain-containing protein", "uniref50_id": "UniRef50_C4XE74", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE74", "UniRef:UniRef50_C4XE74" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE74", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE74" ], "product": "Phospholipid/glycerol acyltransferase domain-containing protein" } }, "id": "OAIKOHHJFB_370", "locus": "GCA000209735_00340" }, { "type": "cds", "sequence": "contig_1", "start": 190700, "stop": 191458, "strand": "+", "frame": 2, "gene": null, "product": "segregation/condensation protein A", "db_xrefs": [ "KEGG:K05896", "RefSeq:WP_013354376.1", "SO:0001217", "UniParc:UPI0001A8C090", "UniRef:UniRef100_C4XE75", "UniRef:UniRef50_A0A449B1R9", "UniRef:UniRef90_C4XE75" ], "nt": "ATGAAAGCTAGTTTATATGCAACAGATGATCATAAATATGACATTAAGTTAGAAAACTTTGATGGACCACTTGATCTTCTATTAGCACTAGTTCAAGAAAAAAATGTTGATATCATGGATGTTGACGTTAGTGAACTTGCTACAGCTTACTTGAAAATTATTCAAAATTTACAAGAGCATGAAATTGATATTGCTGGTGATTATTTAGTTATGGCTGCTACACTTTTAGCTCTTAAAACTAAAATGCTTCTCTTTACTCCAGAAGAAAAACCAGAAATTGAAGAAGACAAACGAGAAATCTTACGCCGTCTTTATGAATACCAACAATTCAAAGAAATTTCACAAACACTTAGACAACAAGAAGCTTTACGTCATGAAATCTTTATTAAGAGTCCAAGTGATGTTGAAGAATTTATGGTTGATGATGACTCAACTGCACTTGATGGAGCTTCAAATCCACTTAAGTTAATAACAACACTTAGAAAAATGTTTGAGCGTACTTACGCAATGCAGCTTAGAAGAACTAAGCTTGAAACATTTAACTTAACACCTAAGGATCAAATACCTTTTATCTTAAACTTATTTGACCACTGTGACACAGTCACATTCGAAATGATTTTTAAACAACCATCATTAAATCACTTCGTTATCACACTTTTAGCGGTTTTAGACTTATCTAGAAGACAAATTATTATATTAAAACAAAATGAACAATTTGGCACAATTACATTTGAAAAGGGGCCTGAATATGAAAAATAA", "aa": "MKASLYATDDHKYDIKLENFDGPLDLLLALVQEKNVDIMDVDVSELATAYLKIIQNLQEHEIDIAGDYLVMAATLLALKTKMLLFTPEEKPEIEEDKREILRRLYEYQQFKEISQTLRQQEALRHEIFIKSPSDVEEFMVDDDSTALDGASNPLKLITTLRKMFERTYAMQLRRTKLETFNLTPKDQIPFILNLFDHCDTVTFEMIFKQPSLNHFVITLLAVLDLSRRQIIILKQNEQFGTITFEKGPEYEK", "aa_hexdigest": "74a996ae10002adb3c97e3bb0b11aa93", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001A8C090", "ncbi_nrp_id": "WP_013354376.1", "uniref100_id": "UniRef100_C4XE75", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C090", "RefSeq:WP_013354376.1", "UniRef:UniRef100_C4XE75" ] }, "ips": { "uniref100_id": "UniRef100_C4XE75", "uniref90_id": "UniRef90_C4XE75" }, "psc": { "uniref90_id": "UniRef90_C4XE75", "product": "segregation/condensation protein A", "uniref50_id": "UniRef50_A0A449B1R9", "kegg_orthology_id": "K05896" }, "pscc": { "uniref50_id": "UniRef50_A0A449B1R9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B1R9" ], "product": "Segregation and condensation protein A" }, "genes": [], "id": "OAIKOHHJFB_371", "locus": "GCA000209735_00341" }, { "type": "cds", "sequence": "contig_1", "start": 191448, "stop": 192032, "strand": "+", "frame": 3, "gene": "scpB", "product": "SMC-Scp complex subunit ScpB", "db_xrefs": [ "GO:0005515", "GO:0007059", "KEGG:K06024", "RefSeq:WP_013526679.1", "SO:0001217", "UniParc:UPI0001F59D37", "UniRef:UniRef100_C4XE76", "UniRef:UniRef50_Q6KHG8", "UniRef:UniRef90_C4XE76" ], "nt": "ATGAAAAATAAAATATTAGAAGCTTTACTATATGTTCAAGGAGATGAAGGATTAACATTAATCCAAGTTAAAGAAATCTTTGGACTTAACACAATTGCAGAAGCTAGAAAAGTTATGAATGATTTTATTAAATCATACAATGATGCTGATGGTGCATTAAAAATAGTTAACTTCAATGAAGTTTATAAATTAGCTACTCGTGAAACATATAAAGAATACATTACCAAAATGGTGCAAGTAGTTAAAAAACACCGCTTATCAAATGCTGCTATTGAAGTTGCTGGAATTACAGCTTATAAACAACCAGTTACACGTTCAATGATTAACAACATTCGTGGTGTTCAAAGTGATCAAGTTTTAAACTCACTTTTAACAAAAGGTGTTATTGAAGAAGTCGGAATTAGCCCTACACCAGGTAACCCAATTCTTTATGGAATTACAAACAAGTTTTATGATTACTTTAAAATTAAATCATTAGCTGAATTACCAAAATTAACAGAATTTAACTTTGTTGATGGTGTTGAAAATGAAGCTGAAGACTTTAACTTATTTGATAGCCAAAGACCAGAAAACTATGATAATTAA", "aa": "MKNKILEALLYVQGDEGLTLIQVKEIFGLNTIAEARKVMNDFIKSYNDADGALKIVNFNEVYKLATRETYKEYITKMVQVVKKHRLSNAAIEVAGITAYKQPVTRSMINNIRGVQSDQVLNSLLTKGVIEEVGISPTPGNPILYGITNKFYDYFKIKSLAELPKLTEFNFVDGVENEAEDFNLFDSQRPENYDN", "aa_hexdigest": "7ddafeacf8dce10e28c72552807be4df", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001F59D37", "ncbi_nrp_id": "WP_013526679.1", "uniref100_id": "UniRef100_C4XE76", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59D37", "RefSeq:WP_013526679.1", "UniRef:UniRef100_C4XE76" ] }, "ips": { "uniref100_id": "UniRef100_C4XE76", "uniref90_id": "UniRef90_C4XE76" }, "psc": { "uniref90_id": "UniRef90_C4XE76", "gene": "scpB", "product": "SMC-Scp complex subunit ScpB", "uniref50_id": "UniRef50_Q6KHG8", "kegg_orthology_id": "K06024", "go_ids": [ "GO:0005515", "GO:0007059" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KHG8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KHG8" ], "product": "Segregation and condensation protein B" }, "genes": [ "scpB" ], "id": "OAIKOHHJFB_372", "locus": "GCA000209735_00342" }, { "type": "cds", "sequence": "contig_1", "start": 192086, "stop": 192925, "strand": "+", "frame": 2, "gene": null, "product": "RNA-binding S4 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AI96", "UniRef:UniRef90_C4XE77" ], "nt": "ATGCTTGAATTTATTGCAACCAAAAATGATGATGGCCGTAAATTAATTAGCTATTTAAAACGAATATTAATTAATACGCCAACATCACATATTTACAAAATTTTGCGTAATAAAGATATTAAAATTAATGGTGTTAGAATCAATGATCCTCAATACATAATTAATGAAAAAGATATCATACAAATTTATGGAATTGAGGCTAATGATTACCAAAATAAGAAGTTTGAAAATAGTGCAAAAAAAGATTTTAAAATCATTTATGAAGATGACAATATTTTGATTGTTTCTAAGCCAATAAACTTAGCTGTTCATTCAGAAAATAATTGTCTAGATAAACAAGTTTTAAGTTACTTAAAATATAATCAAAAAGATAGTTTTAAACCAAGTCATGTTGGACGTCTAGACAAAGTGACAAGTGGATTAATGATATATGCAAAAAATTATGCTACTCTTGTCGAATTAAACTATAAAACAGGTTTTTTTGAAAAAATATATAAATTAAAAAGTGATTTTAATGAAGTTAAAAAACTTGTAGAAATTAATATTCAGCATGATGAAAAATTACACAAAATGGTTGTTACAAATAATGCTAAAGATCCTTTAGCTAAAACACTTTTTTATACAAAAAATAATGAAATTTTTGCATCTATTTTAACTGGCAAAAAACATCAAATTAGATTAAGCATGTCTCATTTAAAAACACCTATTTATGGAGACATAAAATATGACGGTAAAAAAGCTGACAGATTATATTTGCATTGTTATTCAATAACATTTAAAAATTTAGATAATAGTTTAAAATATTTAAATAATAAAGAATTTAATGACCCTATTAATTGA", "aa": "MLEFIATKNDDGRKLISYLKRILINTPTSHIYKILRNKDIKINGVRINDPQYIINEKDIIQIYGIEANDYQNKKFENSAKKDFKIIYEDDNILIVSKPINLAVHSENNCLDKQVLSYLKYNQKDSFKPSHVGRLDKVTSGLMIYAKNYATLVELNYKTGFFEKIYKLKSDFNEVKKLVEINIQHDEKLHKMVVTNNAKDPLAKTLFYTKNNEIFASILTGKKHQIRLSMSHLKTPIYGDIKYDGKKADRLYLHCYSITFKNLDNSLKYLNNKEFNDPIN", "aa_hexdigest": "363e4e2f86aef649538d891c8121866f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XE77", "query_cov": 1.0, "subject_cov": 0.9425675675675675, "identity": 1.0, "score": 556.0, "evalue": 7.36e-200, "valid": true, "product": "RNA-binding S4 domain-containing protein", "uniref50_id": "UniRef50_A0A449AI96" }, "pscc": { "uniref50_id": "UniRef50_A0A449AI96", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AI96" ], "product": "Ribosomal large subunit pseudouridine synthase C" }, "genes": [], "id": "OAIKOHHJFB_373", "locus": "GCA000209735_00343" }, { "type": "cds", "sequence": "contig_1", "start": 192937, "stop": 193032, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAGTTACAAAGGAAGAACTTAAAAAAATAGTTGCTTCATTAATGCTTGAACCAACTAATGAAGTTTTAGACCAAATTATGACTGAATGA", "aa": "MKKVTKEELKKIVASLMLEPTNEVLDQIMTE", "aa_hexdigest": "cd56f7c12800aee7ae9f920db93b2950", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3590.2747, "isoelectric_point": 5.232790946960448 }, "id": "OAIKOHHJFB_374", "locus": "GCA000209735_00344" }, { "type": "cds", "sequence": "contig_1", "start": 193057, "stop": 193239, "strand": "+", "frame": 1, "gene": null, "product": "Glutamyl-tRNA amidotransferase subunit C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D4XV61", "UniRef:UniRef50_UPI0021AE6C6E", "UniRef:UniRef90_C4XE78" ], "nt": "ATGCTTAAAAAATTAAATACAACAAATGTTAAACCATTAAGTCACATCAATGAAGAAGCTTTAGTAGACTTCTTAAGAGAAGATGTTGAAGACACTTCTTATAGTATCAACAAACAAACTGCATTAAACAATGCTATGGACAAAGATCAAGATTACATTATTACAACAAAGGTGGTGAAATAA", "aa": "MLKKLNTTNVKPLSHINEEALVDFLREDVEDTSYSINKQTALNNAMDKDQDYIITTKVVK", "aa_hexdigest": "8a18ba8f4a1d0b68e9f1595b3dce1efd", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021AE6C6E", "query_cov": 0.9333333333333333, "subject_cov": 1.0, "identity": 0.518, "score": 57.8, "evalue": 6.02e-10, "valid": false, "product": "Glu-tRNAGln amidotransferase C subunit", "uniref50_id": "UniRef50_UPI0021AE6C6E" }, "pscc": { "uniref50_id": "UniRef50_UPI0021AE6C6E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AE6C6E" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 192922, "stop": 193239, "observations": { "mutation": [ 193030 ], "stop": [ 193030 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE78", "query_cov": 1.0, "subject_cov": 0.5714285714285714, "identity": 1.0, "score": 117.0, "evalue": 7.51e-33, "gene_start": 1, "gene_end": 60, "reference_start": 46, "reference_end": 105, "reference_sequence": "MIRRIMKKVTKEELKKIVASLMLEPTNEVLDQIMTEWKVLNKNLNMLKKLNTTNVKPLSHINEEALVDFLREDVEDTSYSINKQTALNNAMDKDQDYIITTKVVK" }, "paralog": false, "identity": 0.9809523809523809, "score": 199.0, "evalue": 1.22e-67, "description": "Internal stop codon at 193030. Nonsense mutation around 193030.", "psc": { "uniref90_id": "UniRef90_C4XE78", "product": "Glutamyl-tRNA amidotransferase subunit C", "uniref50_id": "UniRef50_D4XV61", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE78", "UniRef:UniRef50_D4XV61" ] }, "pscc": { "uniref50_id": "UniRef50_D4XV61", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D4XV61" ], "product": "Glutamyl-tRNA(Gln) and/or aspartyl-tRNA(Asn) amidotransferase, C subunit" } }, "id": "OAIKOHHJFB_375", "locus": "GCA000209735_00345" }, { "type": "cds", "sequence": "contig_1", "start": 193239, "stop": 194141, "strand": "+", "frame": 3, "gene": null, "product": "amidase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D5FF4" ], "nt": "ATGAAACTACAAGTCTTAGGTGATTTTGATTTAGCCCAAGCTGAATTAAAGAAAAACAAAAATAATGCAGTTGCTTATGTTTATGATAAAGCTTTAAAAGAAGGCGATGGTTTATTAAACAATGCAGTCTTTACAATTAAAGAAGTTTTTGCAACTAAAGATGCAAAAACAACAGCTTCGAGCTTAATTTTAGAAAACTTCAAACCTGGCTACAATGCAACCTGTGTGCAAAAATTAATTGATGCAGGTGCTTTAAGAGTTGCAAAAGTTTATTGTGATGAATTGGCTCTTGGTGGCACTGGTACTTTTAGTGCTTTTGGTTTAATTAAAAATCCACTAGATAAAGAAAGATTAGCGGGTGGTTCATCTTCAGGATCTGTAGCTACATTTACAGATAATATTTCTTTTGCACTTGGTTCAGATACTGGTGATAGTGTTAGACTGCCAGCGAGTTATAATGGAAATGTTGGATTTAAACCAAGTTATGGAGCAATTAGTCGTTATGGTATGTTTGCTTATGCTTCAAGCTTAGATACAGTTAGCTACTTTGCACACAATGTTAATGATATTGCAGTGATTTCGCAAGTTTTATTTGGAGTAGACAACAAAGATTTTACTTCTAAAAAAGTTAAAATTGATAATGTCCAAAAACTTCAACCTAAGAAAATTGGAGTTTTGAATGTAAGCAAGAATTTAAGCAACTTTGTTTCAAACAACTTTCAAGAATTAATAGTTAAATTAAAAGCAAATAAAAATATTAAAGTTGAAGTTATTGAGCCTCAAGAAGATTTACTCAATGCAGTTAAAGCAATCTATGACATAATTTCTTATTCAGAAGCGAGCTCAAATTTAGCAAATTTAAATGGTATTGCTTTTGGTTCAAGAAAAGAAGGCGAAAGCTGA", "aa": "MKLQVLGDFDLAQAELKKNKNNAVAYVYDKALKEGDGLLNNAVFTIKEVFATKDAKTTASSLILENFKPGYNATCVQKLIDAGALRVAKVYCDELALGGTGTFSAFGLIKNPLDKERLAGGSSSGSVATFTDNISFALGSDTGDSVRLPASYNGNVGFKPSYGAISRYGMFAYASSLDTVSYFAHNVNDIAVISQVLFGVDNKDFTSKKVKIDNVQKLQPKKIGVLNVSKNLSNFVSNNFQELIVKLKANKNIKVEVIEPQEDLLNAVKAIYDIISYSEASSNLANLNGIAFGSRKEGES", "aa_hexdigest": "63363736c68a2c6f5b2a195d571f1738", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI00215D5FF4", "query_cov": 0.83, "subject_cov": 0.9841897233201581, "identity": 0.51, "score": 230.0, "evalue": 1.47e-71, "valid": false, "product": "amidase family protein", "uniref50_id": "UniRef50_UPI00215D5FF4" }, "pscc": { "uniref50_id": "UniRef50_UPI00215D5FF4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D5FF4" ], "product": "amidase family protein" }, "genes": [], "id": "OAIKOHHJFB_376", "locus": "GCA000209735_00346" }, { "type": "cds", "sequence": "contig_1", "start": 194151, "stop": 194291, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTAACACAAGAAGTCAAGGTTTTGGAAAAATGGTTCAAAGAAGACTTACTTTAGGCACTTTCTTCTTACATTCAGAAAATCAAAAAGATTTATTTTTAAAAGCTCAACAAGGCCGTAGATTATTTAAAGAATATTGA", "aa": "MTNTRSQGFGKMVQRRLTLGTFFLHSENQKDLFLKAQQGRRLFKEY", "aa_hexdigest": "c4269d1ec5cc4fa248b92c99039a2fde", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5508.303, "isoelectric_point": 11.000063133239745 }, "id": "OAIKOHHJFB_377", "locus": "GCA000209735_00347" }, { "type": "cds", "sequence": "contig_1", "start": 194298, "stop": 194444, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCACAAACAATATGACATTGTGATTTATCCAGCTTATGCTGATGTTGCTCCTTATATTAATAAGAAGAATAAAAACTACAAATATATGGAATACATTTTAACTGGATCTAACTTAGCTGGGAATCCATCAATTACAATCCCATGA", "aa": "MHKQYDIVIYPAYADVAPYINKKNKNYKYMEYILTGSNLAGNPSITIP", "aa_hexdigest": "42b402ce2ced37fc65c407ffb3b23ffb", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5525.311700000001, "isoelectric_point": 8.964216041564942 }, "id": "OAIKOHHJFB_378", "locus": "GCA000209735_00348" }, { "type": "cds", "sequence": "contig_1", "start": 194554, "stop": 195441, "strand": "+", "frame": 1, "gene": "gatB", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002963D433" ], "nt": "ATGAATAATTTTGAAGTAATTATTGGAATTGAAATTCACCTTGAGTTAAATACAAAAACTAAAATGTTTTCGCCAGCAAGAATAGATTTTAATGCTGAACCTAATACAACAATGAACCAAATCGATTTAGGTTATCCAGGAACTTTACCTTTAGTTAATAAGGAAGCAGTTTTAAGTGGAATTAAATTAGCCAAAGCATTAAATATGAAAATAGATGACGAATTGCACTTTGATCGTAAAAATTACTTTTATCCTGACTTACCTAAAGGCTACCAAATTACTCAATTTTTAAGACCAATAGGATCAGAAGGTTATGTCACAATTAACACTGATAAAGGACCTAAAAAAATCCAAATTGAAAGAATTCACCTTGAAGAAGATACAGCTCGTCAACACCATGATGAAGAAGGGACTAAATTAGATTACAACCGTGCAGGTGTGCCTTTAATTGAAATAGTTACTCATCCTGTTTTAAGAAGTGCAGATGAAGCCGTAGCTTATGTTGATATGATTCGTAAGATAGCTTTAAGCTTAAATATTAGTGATGCTAAAATGGAACAAGGTAGTTTACGTGCTGATGTTAATGTGTCACTTAGACCATATGGTTACAAAGGTTTTGGTACAAAAGTTGAAATCAAAAACATGAACTCATTTAGAGCAATCAAGAATGCTCTTGAATTTGAAATTGATTTTCAAAGAAAACAAATTTTGACCAATAAGCCTATTTTACAGCAAACTAAGCGTTATAATGAAGCTACTTTAAGTACAGTTGTAATGCGTACAAAAACAGGTGAAGTTGATTATAAATACTTCCCAGAGCCAAATATTCCATTTATTAAATTAAGCAAAAAATTAATTGATTCAGTTAAATTAAATGAACTTCCTTGA", "aa": "MNNFEVIIGIEIHLELNTKTKMFSPARIDFNAEPNTTMNQIDLGYPGTLPLVNKEAVLSGIKLAKALNMKIDDELHFDRKNYFYPDLPKGYQITQFLRPIGSEGYVTINTDKGPKKIQIERIHLEEDTARQHHDEEGTKLDYNRAGVPLIEIVTHPVLRSADEAVAYVDMIRKIALSLNISDAKMEQGSLRADVNVSLRPYGYKGFGTKVEIKNMNSFRAIKNALEFEIDFQRKQILTNKPILQQTKRYNEATLSTVVMRTKTGEVDYKYFPEPNIPFIKLSKKLIDSVKLNELP", "aa_hexdigest": "d6148eafd332b0aedca401f8909b698b", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_UPI002963D433", "query_cov": 0.9830508474576272, "subject_cov": 0.8787878787878788, "identity": 0.541, "score": 298.0, "evalue": 2.51e-97, "valid": false, "gene": "gatB", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB", "uniref50_id": "UniRef50_UPI002963D433", "go_ids": [ "GO:0016884" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002963D433", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002963D433" ], "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB" }, "genes": [ "gatB" ], "id": "OAIKOHHJFB_379", "locus": "GCA000209735_00349" }, { "type": "cds", "sequence": "contig_1", "start": 195667, "stop": 195804, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02637.23" ], "nt": "ATGGACCAAGATATTATTTCAGGTCGTTCATTTAAAAAACTTGTTCCTTTATTAGTTAATTTTGATGGTGATTTTGACAAATTAGTTGTTGATAATAATCTTAAACAAATTAGCGATGAAGCCACAATTACAAAATGA", "aa": "MDQDIISGRSFKKLVPLLVNFDGDFDKLVVDNNLKQISDEATITK", "aa_hexdigest": "61620e15f46e48a5677bbd62617ae68e", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02637.23", "name": "GatB_Yqey", "length": 44, "aa_cov": 0.9777777777777777, "hmm_cov": 1.0, "evalue": 1.4543829122296144e-07, "score": 27.535066604614258, "start": 2, "stop": 45 } ], "seq_stats": { "molecular_weight": 5081.7497, "isoelectric_point": 4.609039878845214 }, "id": "OAIKOHHJFB_380", "locus": "GCA000209735_00350" }, { "type": "cds", "sequence": "contig_1", "start": 196105, "stop": 196692, "strand": "+", "frame": 1, "gene": null, "product": "Abortive infection protein AbiGI", "db_xrefs": [ "RefSeq:WP_013354381.1", "SO:0001217", "UniParc:UPI0001A8C096", "UniRef:UniRef100_C4XE81", "UniRef:UniRef50_C4XE81", "UniRef:UniRef90_C4XE81" ], "nt": "ATGCAAGAAGAAAAAGTTATTAAAATTGGAAAAAAACACAATGGTGTAATTACAACAAAGGAGTGCGCAATGCAAAGAATTCACAATCAAACATTACACAGGTTATTGTCAAAAAAAATTATTGAAAAAATTTCCCATGGTATTTATTTATTGACCGAGTATTTAGAAGATGATCATTATTTGTTTCAATTAAGATACCCAAAGACAATTTTTTCATATGAAACAGCCTTATATTTAATTCACGAAACAGACTTTTTACCTATAAATTTTGATGTTTCTGTAACAAAAGGGTACAAATTCAATGATAAAGAAAATATAAGAGTTAATTATGTTTCTGATGAGTTAGCTGATTTAGGAAAAATAAAAGTTAAAACAATGTTTAATAATGATGTTTATGTTTATTGTTATGAAAGAGTATTGTGCGATTTAATAAAAAATAGAAAAAAAGCAAATTTAGAAACTTATGTTAATGCAATTAAAAGATATGCACAATATAAAAATAAAAATTTGGATTTATTGTATGAAATAGCTAAGAAAATGAATATTTTAGATGAAATGAAAAATATTATGATCGGAATAATTTATTAA", "aa": "MQEEKVIKIGKKHNGVITTKECAMQRIHNQTLHRLLSKKIIEKISHGIYLLTEYLEDDHYLFQLRYPKTIFSYETALYLIHETDFLPINFDVSVTKGYKFNDKENIRVNYVSDELADLGKIKVKTMFNNDVYVYCYERVLCDLIKNRKKANLETYVNAIKRYAQYKNKNLDLLYEIAKKMNILDEMKNIMIGIIY", "aa_hexdigest": "0f07e925844bbe441d709750411fb93b", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C096", "ncbi_nrp_id": "WP_013354381.1", "uniref100_id": "UniRef100_C4XE81", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C096", "RefSeq:WP_013354381.1", "UniRef:UniRef100_C4XE81" ] }, "ips": { "uniref100_id": "UniRef100_C4XE81", "uniref90_id": "UniRef90_C4XE81" }, "psc": { "uniref90_id": "UniRef90_C4XE81", "product": "Abortive infection protein AbiGI", "uniref50_id": "UniRef50_C4XE81" }, "pscc": { "uniref50_id": "UniRef50_C4XE81", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE81" ], "product": "Abortive infection protein AbiGI" }, "genes": [], "id": "OAIKOHHJFB_381", "locus": "GCA000209735_00351" }, { "type": "cds", "sequence": "contig_1", "start": 196694, "stop": 196879, "strand": "+", "frame": 2, "gene": null, "product": "Nucleotidyl transferase AbiEii/AbiGii toxin family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE82", "UniRef:UniRef90_C4XE82" ], "nt": "ATGACAATAGAAAAAATTAAAATGATAAGCAAGGAAATAAGCAAAAAAACTAACATACCATTTAATAAAATATTTCATAATTATTTTCTTGAATTAATTTTAAGAAAAATATCTAAAAGCAAATATTCAAATTTTTTTGTATTAAAAGGCGGATACTTAATAACAACAATTTTTAACTATAAATGA", "aa": "MTIEKIKMISKEISKKTNIPFNKIFHNYFLELILRKISKSKYSNFFVLKGGYLITTIFNYK", "aa_hexdigest": "ce0a3ea49624b32cb75c3919e102d53f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 196688, "stop": 197011, "observations": { "mutation": [ 196877 ], "stop": [ 196877 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE82", "query_cov": 1.0, "subject_cov": 0.5700934579439252, "identity": 1.0, "score": 120.0, "evalue": 5.02e-34, "gene_start": 1, "gene_end": 61, "reference_start": 3, "reference_end": 63, "reference_sequence": "MNMTIEKIKMISKEISKKTNIPFNKIFHNYFLELILRKISKSKYSNFFVLKGGYLITTIFNYKWRTTVDLDFMSIFKNKNLGEIKDIVNKILNEKDENDTIYKIYKT" }, "paralog": false, "identity": 0.9813084112149533, "score": 209.0, "evalue": 7.64e-72, "description": "Internal stop codon at 196877. Nonsense mutation around 196877.", "psc": { "uniref90_id": "UniRef90_C4XE82", "product": "Nucleotidyl transferase AbiEii/AbiGii toxin family protein", "uniref50_id": "UniRef50_C4XE82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE82", "UniRef:UniRef50_C4XE82" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE82" ], "product": "Nucleotidyl transferase AbiEii/AbiGii toxin family protein" } }, "id": "OAIKOHHJFB_382", "locus": "GCA000209735_00352" }, { "type": "cds", "sequence": "contig_1", "start": 196904, "stop": 197011, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAATTTTTAAAAACAAAAATTTAGGTGAAATAAAAGACATTGTTAATAAAATTTTGAATGAAAAAGATGAAAATGACACTATTTACAAAATATATAAAACATAA", "aa": "MSIFKNKNLGEIKDIVNKILNEKDENDTIYKIYKT", "aa_hexdigest": "4ec8de24b34104968cd2f97803fa8802", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4173.783000000001, "isoelectric_point": 8.007634162902832 }, "id": "OAIKOHHJFB_383", "locus": "GCA000209735_00353" }, { "type": "cds", "sequence": "contig_1", "start": 197048, "stop": 197242, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATGTAAAAGTAAAAATAAAAAACATAACAGAAATTGTTCCTTTAGACATTGTTGAAGAATCAAAAATTGATCCTACTTATATTTCAAAAGAATTTAATTCTATTTATGGTGAAAATTTTACTTTTCTAATTTATAACATTGAAAATGTAATTGCTGAAAAGGTTTTTGCTATTTATACCAACCGTTTTTGA", "aa": "MYVKVKIKNITEIVPLDIVEESKIDPTYISKEFNSIYGENFTFLIYNIENVIAEKVFAIYTNRF", "aa_hexdigest": "bfc267f768b0b7193ae3276e881156a2", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7569.673999999996, "isoelectric_point": 4.9316011428832995 }, "id": "OAIKOHHJFB_384", "locus": "GCA000209735_00354" }, { "type": "cds", "sequence": "contig_1", "start": 197771, "stop": 198064, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAAACTAGTTGAAAAATATATTCAACAAGCTGGATATGTTGAAAACAAAACATATTTTAAACAAATATATTCAAGTGAGATCTATGAAAAGTTTGGAATTAATTTAAATAATATTCAAGAAACAACTGCAAATAAAAGATTTGATTTTGGAATCATTAAAAATCATAAATTTTATGCAATTGAAGTAAATTTTTATACGGCTCAAGGTTCTAAACTTAATGAAGTTTCAAGAAGCTATGAAAAAATTTATTTATCTACAAAAACAATTACTAATTTTGAATTCATTTGA", "aa": "MEKLVEKYIQQAGYVENKTYFKQIYSSEIYEKFGINLNNIQETTANKRFDFGIIKNHKFYAIEVNFYTAQGSKLNEVSRSYEKIYLSTKTITNFEFI", "aa_hexdigest": "f4fe23e10362a9884fb675aa0d21918a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11564.959199999988, "isoelectric_point": 8.730066871643068 }, "id": "OAIKOHHJFB_385", "locus": "GCA000209735_00355" }, { "type": "cds", "sequence": "contig_1", "start": 198507, "stop": 199556, "strand": "-", "frame": 3, "gene": null, "product": "Site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "EC:2.1.1.72", "KEGG:K06223", "RefSeq:WP_041594275.1", "SO:0001217", "UniParc:UPI0002ECF00F", "UniRef:UniRef100_C4XE85", "UniRef:UniRef50_A0A1V5HP79", "UniRef:UniRef90_C4XE85" ], "nt": "ATGGAAACACTTTTTAAAACAATTGGCGACAATATTAAAATGGAAAGACTTGCAAAAAATATGACACAACAAGAATTAGCAGAATTAAGTAGCACCGAAAGATCTCAATTAACTAAAATTGAAAAAGGTCAAATTAAGGGCGTTCAGTTTTTTACAATTTGCAAAATATATAATGCTTTGAATTTGAATAATATTTATATTGGAAAATTAAACACAAAACTTTCACCATTTGTAAAATGGGCTGGTGGCAAAACTCAATTATTAGATAAACTTAAAAGTTTAATGCCAAAAACATATAACAATTATTTGGAACCTTTTGTTGGTGGCGGAGCATTATTACTAAATCTAAAGCCGCAAAATTTCATAATAAATGATTTTAATAAAGAATTAATTAATGTTTTTAATTGCTTTAAAAATGATAAAGATTTTAAATTATTAAAAAAAGAATTAATTAATCATGAAAACAATCATAATGATGGGTATTACTATAAAATAAGAGATTTGGACAAATTAGATAAATACGATAAACTGCCAAATTACAAAAAAGCTGCCCGAACAATATACTTAAATAAAGCGTGTTTTAATGGTTTATATCGAGTAAATTCTAAAGGACAATTTAATGTACCTTCTGGCAAGAAAAACTCAGTTAATTGCTTTGATAGAGACAATTTTGAAAATTTAAAAAACTTTTTAAAAACTACAAATAATCAAATTTTCTGTGAAGATTTTGAAGTATTTTGTGAAAAAGCTCAAAAAGGAGATTTTGTTTATTTTGATCCTCCTTATGATGTCATTGAAAACAAAAATACTTTCACTTCATATAATTCAGAAAGTTTTGGAAAAGAAGAACAAAAACGATTATCCGAAGTTTTTAAAAAACTTGACAAAAAAGGTGTCAAAGTAATGTTAAGTAATCACAACACAAAATATATTAATGAATTATATGCAGACTTTAATATTAATGTTGTTAAAGCAAAAAGAATGATAAATTCAAATGGAAGTGGACGAGGAGAAGTTGAAGAAGTAATAATTACAAATTATAGGGAGTAA", "aa": "METLFKTIGDNIKMERLAKNMTQQELAELSSTERSQLTKIEKGQIKGVQFFTICKIYNALNLNNIYIGKLNTKLSPFVKWAGGKTQLLDKLKSLMPKTYNNYLEPFVGGGALLLNLKPQNFIINDFNKELINVFNCFKNDKDFKLLKKELINHENNHNDGYYYKIRDLDKLDKYDKLPNYKKAARTIYLNKACFNGLYRVNSKGQFNVPSGKKNSVNCFDRDNFENLKNFLKTTNNQIFCEDFEVFCEKAQKGDFVYFDPPYDVIENKNTFTSYNSESFGKEEQKRLSEVFKKLDKKGVKVMLSNHNTKYINELYADFNINVVKAKRMINSNGSGRGEVEEVIITNYRE", "aa_hexdigest": "1d5dc5de2bf46fa4d728d379609e56fe", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0002ECF00F", "ncbi_nrp_id": "WP_041594275.1", "uniref100_id": "UniRef100_C4XE85", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002ECF00F", "RefSeq:WP_041594275.1", "UniRef:UniRef100_C4XE85" ] }, "ips": { "uniref100_id": "UniRef100_C4XE85", "uniref90_id": "UniRef90_C4XE85" }, "psc": { "uniref90_id": "UniRef90_C4XE85", "product": "Site-specific DNA-methyltransferase (adenine-specific)", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A1V5HP79", "kegg_orthology_id": "K06223" }, "pscc": { "uniref50_id": "UniRef50_A0A1V5HP79", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5HP79" ], "product": "Site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "OAIKOHHJFB_386", "locus": "GCA000209735_00356" }, { "type": "cds", "sequence": "contig_1", "start": 199643, "stop": 199813, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13412.12", "PFAM:PF13730.12", "PFAM:PF01978.25" ], "nt": "TTGCCAGATTCTGCTTATTATGTATATATGGCTTTAAAAAATAATCCAAAAGAAACAGCTGGTTCACTAGCTAAAAAAATAAAAAAATCTTCGAGAACTATTCAAAGAATTTTAGATCTTTTAAAAGACAAGGGCTACATAGCTAGAATTGGTAAGACAAGAGGATATTGA", "aa": "MPDSAYYVYMALKNNPKETAGSLAKKIKKSSRTIQRILDLLKDKGYIARIGKTRGY", "aa_hexdigest": "7ef3bf57f3b285762e7fce32fe31178b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13412.12", "name": "HTH_24", "length": 37, "aa_cov": 0.6607142857142857, "hmm_cov": 1.0, "evalue": 1.5698859766615403e-06, "score": 23.758155822753906, "start": 11, "stop": 47 }, { "id": "PF13730.12", "name": "HTH_36", "length": 35, "aa_cov": 0.5714285714285714, "hmm_cov": 1.0, "evalue": 2.742173608943454e-07, "score": 26.415742874145508, "start": 16, "stop": 47 }, { "id": "PF01978.25", "name": "TrmB", "length": 45, "aa_cov": 0.8035714285714286, "hmm_cov": 1.0, "evalue": 4.377727857731836e-07, "score": 25.81563377380371, "start": 8, "stop": 52 } ], "seq_stats": { "molecular_weight": 6365.430400000001, "isoelectric_point": 10.118457984924316 }, "id": "OAIKOHHJFB_387", "locus": "GCA000209735_00357" }, { "type": "cds", "sequence": "contig_1", "start": 199814, "stop": 199993, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAATTTAGTTGATGAAGCTAAAATTTGTAATAATGGTTTCAAGAGAGTATATGAAGAATGTGAAAAAACTAATGCTAAAATTACTTATCAAAAAGGCAATTATGGTTTTGCATTTATATTTTTAAGAGAAAACAAAAGTTTAATTAGTAAAGATGAAGAATTTAAAGAATACTGA", "aa": "MINLVDEAKICNNGFKRVYEECEKTNAKITYQKGNYGFAFIFLRENKSLISKDEEFKEY", "aa_hexdigest": "415fb4a15b0c0f217fed02bb453cd76a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7021.913099999998, "isoelectric_point": 6.222414588928221 }, "id": "OAIKOHHJFB_388", "locus": "GCA000209735_00358" }, { "type": "cds", "sequence": "contig_1", "start": 200237, "stop": 200422, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATGAAAAACTAACAAATGCTGCTCATTATCTTTTTTCGCATAGAGAACCTATAGTTTTAAAAATGGCTGTTTTTGCAACAGATCAAAAATTAACATTTTTAGATATCAATAGAATTTCTGGTAACATCTTTCAATTAATTGAGAAAGCTAATGAATATGTTAAATCTAAAATCAATTGA", "aa": "MKNEKLTNAAHYLFSHREPIVLKMAVFATDQKLTFLDINRISGNIFQLIEKANEYVKSKIN", "aa_hexdigest": "f8413889cba5f0f0837b8715b90f8b0b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7096.193599999999, "isoelectric_point": 9.455529594421389 }, "id": "OAIKOHHJFB_389", "locus": "GCA000209735_00359" }, { "type": "cds", "sequence": "contig_1", "start": 200561, "stop": 200959, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04326.19" ], "nt": "ATGAATTTTGGAATCGAAAGCGAAACACTTGAATTTAAGAAAAATACAAATGAAATAAATGAGGCTATGAAATCAATTTGTGCCATGTTAAATAAACACGGAAAAGGCACATTATATTTTGGCATCGGAGATGATGGATTAGTATATGATCAAAAAATCAACATAAATACTTTAAGAGATATTTGCAGAAAAATTTATGAAAATATTGAACCTGTAATTAGTCCTATTATTGAGAAAAAAATTGTTGATAATAAACAAATAATAGAGGTAACTTTTTGTGGGAATGATGCTCCATATTCAGTTGAAGGCATCTATTATATTAGAAATGATGATGAGGATAAAATACTCAGTCCTAATCAATTAAGACAGCTTTTTGAATTCAATAAAAACAAAAGCTGA", "aa": "MNFGIESETLEFKKNTNEINEAMKSICAMLNKHGKGTLYFGIGDDGLVYDQKININTLRDICRKIYENIEPVISPIIEKKIVDNKQIIEVTFCGNDAPYSVEGIYYIRNDDEDKILSPNQLRQLFEFNKNKS", "aa_hexdigest": "c41e6403b0996c4f92f8cefef0c78054", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04326.19", "name": "SLFN_AlbA_2", "length": 117, "aa_cov": 0.8333333333333334, "hmm_cov": 0.9914529914529915, "evalue": 3.8096127471611764e-21, "score": 71.92584991455078, "start": 6, "stop": 115 } ], "seq_stats": { "molecular_weight": 15230.18459999999, "isoelectric_point": 5.072220802307128 }, "id": "OAIKOHHJFB_390", "locus": "GCA000209735_00360" }, { "type": "cds", "sequence": "contig_1", "start": 201108, "stop": 201395, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACAATACAAAAACATAAAAGAAGTTGAAGAAAAATACACTTTTGATCTTGAAGATATCTTAAAAGGGCAAACAATTTATGATTTATTTAATGAGTTTATTGAGCTTTCTAAAATAGCTTTAAAAACAAAAGACACAAAATATGAAAATGTCGAAAACTACATTAAATCAGTTGAATTGGGAAAAGATATGGGAGTTTTATCTAATAGAATTCACAATTATCTTTCTAATCATAGTAATCAAAATTTAGTAGATTCTAAATGGACTGATTTAAGCACAAAATGA", "aa": "MKQYKNIKEVEEKYTFDLEDILKGQTIYDLFNEFIELSKIALKTKDTKYENVENYIKSVELGKDMGVLSNRIHNYLSNHSNQNLVDSKWTDLSTK", "aa_hexdigest": "570e72111223cc751911b6ccbc54df73", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11240.561299999988, "isoelectric_point": 5.662323188781738 }, "id": "OAIKOHHJFB_391", "locus": "GCA000209735_00361" }, { "type": "cds", "sequence": "contig_1", "start": 201573, "stop": 202151, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATCAAAGTTCAAGTGCAAATCCTGACTATGAATCTATTTTTAATATTATTTCAGATAGCGAATTAGACTATGGTTTTGCAACTGATTCAAAAGGAAAAAAATATAAATTGTCACCAGCAATTAAAGCAAAATTTATGAAAAAAGATGATTTTAAAATTAGAAAATCAACACGTGATTCATTTTTAAAAGCGCACATGAAACATAAAGATTCAATGGCTAACTTACTTTATCAACATTTCAAAGGATTAACTGTAACTGCAAAAATACGTAAATACAAAAATACTATTGATATGTTAACTCACAGTGATAAAGTAGATGATTCTATGTTGCAATTTTTATTTGACAAAGTGTCGACATTAAAACATACAATAAAAAATAGAAACAAGTATTACAAAAAGTTTTATGAAGCAAAATTTAAAGAAAAATATCATGCAAAATATGATTCATATCGTGAATTAGTCAAAGTTAAAAGTACTTACACAGTTGAACAAATGCAAAATATTGTTTCAGAAGCTCTTAAACCTTTTGGATCTGAATACCACAAAATGATTACTAAAGCAATTAATGAAAGATGA", "aa": "MNQSSSANPDYESIFNIISDSELDYGFATDSKGKKYKLSPAIKAKFMKKDDFKIRKSTRDSFLKAHMKHKDSMANLLYQHFKGLTVTAKIRKYKNTIDMLTHSDKVDDSMLQFLFDKVSTLKHTIKNRNKYYKKFYEAKFKEKYHAKYDSYRELVKVKSTYTVEQMQNIVSEALKPFGSEYHKMITKAINER", "aa_hexdigest": "a42a758137a74d58059930eb56efc4a3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22652.824700000012, "isoelectric_point": 9.68361873626709 }, "id": "OAIKOHHJFB_392", "locus": "GCA000209735_00362" }, { "type": "cds", "sequence": "contig_1", "start": 202161, "stop": 202499, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGTTGATAACAAACTTTCAGGAGCTTATAGCATCGGCAATACTTATGGTTTGGATAAGAAATATATTTTAATGAATTTTGATGGTGAATTAGGTTCAGTTGAAACTTTAGCTCATGAATTAGGCCACTCAATGCATTCTTATTTTTCAGATAAAAATAATGATATAAGTAATGCTTCTTATCCAATTATTTTGGCTGAAATAGCTTCAATATTTAATGAACTTATGCTTTATAACTATTTATTAAAAACAAGTAAAAATGATTTATTTAAATTTAAAATTTTGGACAACATGATTGACGGGTTTGTTGGAACCGTATTTAGACAAATACTTTGA", "aa": "MTVDNKLSGAYSIGNTYGLDKKYILMNFDGELGSVETLAHELGHSMHSYFSDKNNDISNASYPIILAEIASIFNELMLYNYLLKTSKNDLFKFKILDNMIDGFVGTVFRQIL", "aa_hexdigest": "4883482d946ef3c0ce0354fe6dd4bc16", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12672.344699999985, "isoelectric_point": 5.1619695663452125 }, "id": "OAIKOHHJFB_393", "locus": "GCA000209735_00363" }, { "type": "cds", "sequence": "contig_1", "start": 202593, "stop": 202898, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATATGCAACCAAGAAAATTAAATACAAAAAAGAAAAAAATATTATGTCGGTTTATGTACCTCATTATTACTACGGATTTTACGTATACAAGTATGCTATAGGTCAATTGGTTGCAAATTACTTTTACACAAGAGTCAAAAATGAAGGCGAAAAATACTTGCAAGTTTACATCAATGATTTCTTAAGTGCTGGAGATAGAGATTATCCTCTTGAAACACTTAAAAAAGTTGGTGCTGACTTAAAAGATCCTCAATTTTATGAAATTGGTTTTAGTTATTTTAAAGAAATAGTTAAAGAATGA", "aa": "MKYATKKIKYKKEKNIMSVYVPHYYYGFYVYKYAIGQLVANYFYTRVKNEGEKYLQVYINDFLSAGDRDYPLETLKKVGADLKDPQFYEIGFSYFKEIVKE", "aa_hexdigest": "4d2b96b4d73b5534a500c5b01b53f353", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12170.87519999999, "isoelectric_point": 9.17128849029541 }, "id": "OAIKOHHJFB_394", "locus": "GCA000209735_00364" }, { "type": "cds", "sequence": "contig_1", "start": 203241, "stop": 203381, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTAATATTTGCATTTAGAAATCAAAAAAGATATAAAAGAGATTTTGCTGATTTTATATTGAAAAAATATAAAAAAGCGACAAAAGCTGCTTTTTTACCTTTTCTTATTTCAACTTTAGCATTTTATTTAGCTTGA", "aa": "MNLIFAFRNQKRYKRDFADFILKKYKKATKAAFLPFLISTLAFYLA", "aa_hexdigest": "e553bb910996a9e17b526de4f0921ddd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5523.583899999999, "isoelectric_point": 10.354412269592284 }, "id": "OAIKOHHJFB_395", "locus": "GCA000209735_00365" }, { "type": "cds", "sequence": "contig_1", "start": 203676, "stop": 203786, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTCAATTATTGCAGCAATTTTAAATATTGTTTTAACATTGTCATTTCAACATTTACATAATCAAATATTTGGTGATTTAAAAAATTTATTACCTATTAAAGAATGA", "aa": "MISIIAAILNIVLTLSFQHLHNQIFGDLKNLLPIKE", "aa_hexdigest": "4ba0ee04945a45c1b7f24c9baa578c93", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4071.8679, "isoelectric_point": 6.703556632995605 }, "id": "OAIKOHHJFB_396", "locus": "GCA000209735_00366" }, { "type": "cds", "sequence": "contig_1", "start": 204312, "stop": 204509, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGAAGAATTATTAAAAGAAAACAAAAGAATAGCTTTCATTCCTAAAATAGATCTTAAAGATTTAGATCCTAAAAAAGGTGCAACATTAGAAATTACATTTCCTCTTTTACCTGATTTTGAAAAAATTCAAATCAAATCACCTAAAACAAAATTTGAATTAATCAAAATTGAAAAGAAAGATATTGAAGATTGA", "aa": "MKEELLKENKRIAFIPKIDLKDLDPKKGATLEITFPLLPDFEKIQIKSPKTKFELIKIEKKDIED", "aa_hexdigest": "378f1d9c066f4018d6a3a668c29f5142", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7653.0238999999965, "isoelectric_point": 7.911253929138184 }, "id": "OAIKOHHJFB_397", "locus": "GCA000209735_00367" }, { "type": "cds", "sequence": "contig_1", "start": 204876, "stop": 205589, "strand": "+", "frame": 3, "gene": "tig", "product": "trigger factor", "db_xrefs": [ "EC:5.2.1.8", "GO:0003755", "GO:0005854", "GO:0015031", "GO:0051082", "GO:0051083", "SO:0001217", "UniRef:UniRef50_C4XE89", "UniRef:UniRef90_C4XE89" ], "nt": "ATGTCAGAAGCTAAAAATGCTGCTGAAGTTAAAAAAGAAGTTCAATTACTTTTAGAAATTGGTGCTTTAGATGAATCTATTGAAAACTATTTTAATGAATTAGTTGAAGAATTTGACAGGAAAAATGATTTTATTATTCATAAAGCATTTATTAAAGAAGATGTTAAAAAATCACTTTCAGAATTAGAAAGTAATTTAAAACAACAAAAAATCAAATTACATGAATATCTTGAATTATTAGGTCAAACAAAAGAACAATTAATGGATTTATTAGCTAAAGACCACTCATCAAAAGTTAAAAAACAAACTATTTCAACTGAAATCGTTAAACAAGTTAAACCTGAATTTCATAAAGATAAATTTGCATTTAGAATTTTATCATTAGCCTATAATACAGGCTTAGACACCAAATTTATTGAAGACACATTTTTTGATGAAAAAGGTGAAATTAAATCAAGTGTTAATGCAATGGACACATTAAAAACATTCATTATTATTGAAAAAGTTTTAGAAAAATTTGACAAAAACGGTTTTGCGACATACTTAAAATCTAAAGAAGCTTTCTCAAAAGAATTAGATAAAAGAGTTAAAGCATATACTAAAAAAAGAGAAGAAGAATTAGCTAAAGGTGAAAAAGAAGCTAAAAAAGAAGAATCTAAACCGCAACCAACAAAAAAAGCTGCTGCCAAAAAAACAACAAAAAATAAAAACTAA", "aa": "MSEAKNAAEVKKEVQLLLEIGALDESIENYFNELVEEFDRKNDFIIHKAFIKEDVKKSLSELESNLKQQKIKLHEYLELLGQTKEQLMDLLAKDHSSKVKKQTISTEIVKQVKPEFHKDKFAFRILSLAYNTGLDTKFIEDTFFDEKGEIKSSVNAMDTLKTFIIIEKVLEKFDKNGFATYLKSKEAFSKELDKRVKAYTKKREEELAKGEKEAKKEESKPQPTKKAAAKKTTKNKN", "aa_hexdigest": "6f712900f68a8c31b92226e2dcb4f1d1", "start_type": "ATG", "rbs_motif": null, "genes": [ "tig" ], "truncated": "5-prime", "pseudogene": { "start": 204576, "stop": 205589, "observations": { "mutation": [ 204720 ], "stop": [ 204720 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE89", "query_cov": 1.0, "subject_cov": 0.4637964774951076, "identity": 1.0, "score": 423.0, "evalue": 7.58e-145, "gene_start": 1, "gene_end": 237, "reference_start": 275, "reference_end": 511, "reference_sequence": "MRRIMAENNKKFTIDEKKSELVYTLEINGKEWEKLYADAKAKEVKNIKIPGFRPGKAPQHEIEKRVNPLSVASEVVDQYWRNNHDRLKEELLKENKRIAFIPKIDLKDLDPKKGATLEITFPLLPDFEKIQIKSPKTKFELIKIEKKDIEDWIAQQLEKNALLLPLKKDEKTKDGDTVTIDYKGFIKDEPFDGGEAQGFDLKLGSHTFIDTFEDQLIGKKIGWKGEVKVTFPKEYAVEKLKGQPATFEVEIKDAKRREKVELTDKNFGTVRLAKMSEAKNAAEVKKEVQLLLEIGALDESIENYFNELVEEFDRKNDFIIHKAFIKEDVKKSLSELESNLKQQKIKLHEYLELLGQTKEQLMDLLAKDHSSKVKKQTISTEIVKQVKPEFHKDKFAFRILSLAYNTGLDTKFIEDTFFDEKGEIKSSVNAMDTLKTFIIIEKVLEKFDKNGFATYLKSKEAFSKELDKRVKAYTKKREEELAKGEKEAKKEESKPQPTKKAAAKKTTKNKN" }, "paralog": false, "identity": 0.9970326409495549, "score": 654.0, "evalue": 4.76e-238, "description": "Internal stop codon at 204720. Nonsense mutation around 204720.", "psc": { "uniref90_id": "UniRef90_C4XE89", "gene": "tig", "product": "trigger factor", "ec_ids": [ "5.2.1.8" ], "uniref50_id": "UniRef50_C4XE89", "go_ids": [ "GO:0003755", "GO:0005854", "GO:0015031", "GO:0051082", "GO:0051083" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE89", "EC:5.2.1.8", "UniRef:UniRef50_C4XE89", "GO:0003755", "GO:0005854", "GO:0015031", "GO:0051082", "GO:0051083" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE89", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE89" ], "product": "Trigger factor" } }, "id": "OAIKOHHJFB_398", "locus": "GCA000209735_00368" }, { "type": "cds", "sequence": "contig_1", "start": 205716, "stop": 206024, "strand": "+", "frame": 3, "gene": null, "product": "tRNA/rRNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH69" ], "nt": "ATGCTTAATATTGTTTTATTTGAACCAGAAATATCACCTAACACAGGAAATATAATTAGAACCTGTTTTGCACTTGGAGCAAAACTTCATATTATTAAACCAATTGGTTTTGATTTAGATCCTAAGTATTTATCGCGTCCTGCAGCAGGCAGATTATTAAGTGATATTCAACATGAAGTGCACGCTTCGTATAAAGATTTTTATGAAAAATATAAAAACAAAAATATCTTTTATTTAACTCGTTATGGTTTAAAAACTTATACAGATGTTGATTTTAAAAAAGAATACAAAAAGAATAAAGAAGTTTGA", "aa": "MLNIVLFEPEISPNTGNIIRTCFALGAKLHIIKPIGFDLDPKYLSRPAAGRLLSDIQHEVHASYKDFYEKYKNKNIFYLTRYGLKTYTDVDFKKEYKKNKEV", "aa_hexdigest": "22b6a188d1e5174ba9a3513e0f65bdfc", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A449AH69", "query_cov": 0.9607843137254902, "subject_cov": 0.9514563106796117, "identity": 0.7240000000000001, "score": 150.0, "evalue": 1.55e-45, "valid": false, "product": "tRNA/rRNA methyltransferase", "ec_ids": [ "2.1.1.207" ], "uniref50_id": "UniRef50_A0A449AH69", "kegg_orthology_id": "K03216" }, "pscc": { "uniref50_id": "UniRef50_A0A449AH69", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH69" ], "product": "tRNA/rRNA methyltransferase" }, "genes": [], "id": "OAIKOHHJFB_399", "locus": "GCA000209735_00369" }, { "type": "cds", "sequence": "contig_1", "start": 206028, "stop": 206249, "strand": "+", "frame": 3, "gene": "trmH", "product": "TrmH family RNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH15" ], "nt": "ATGTTTGGAAAAGAATCAACTGGAATTGACAAAGAAATTTTGCAAACTCATTTAGAAAATTGCTTAAGAATACCTATGATGAGTGAAATGAGATCAATCAATTTAGCCAATACTGTTGCAATAGTAGGTTTTGAAATTATGCGTCAATTAGATTTTAAAGAGTTAAGTCTATATGAAGTTCAAAAAGGAAAGGACTTCTTAATCAAATGGAAAAAACAGTAA", "aa": "MFGKESTGIDKEILQTHLENCLRIPMMSEMRSINLANTVAIVGFEIMRQLDFKELSLYEVQKGKDFLIKWKKQ", "aa_hexdigest": "57d40f84f2e8ab4431ba3a570ef850f3", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_A0A449AH15", "query_cov": 0.958904109589041, "subject_cov": 0.8433734939759037, "identity": 0.643, "score": 94.7, "evalue": 4.76e-24, "valid": false, "product": "TrmH family RNA methyltransferase", "uniref50_id": "UniRef50_A0A449AH15" }, "pscc": { "uniref50_id": "UniRef50_A0A449AH15", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH15" ], "product": "TrmH family RNA methyltransferase" }, "genes": [ "trmH" ], "id": "OAIKOHHJFB_400", "locus": "GCA000209735_00370" }, { "type": "cds", "sequence": "contig_1", "start": 206234, "stop": 206947, "strand": "+", "frame": 2, "gene": "spoU", "product": "SpoU rRNA Methylase family", "db_xrefs": [ "RefSeq:WP_013354390.1", "SO:0001217", "UniParc:UPI0001E32F90", "UniRef:UniRef100_C4XE91", "UniRef:UniRef50_A0A3P8KX10", "UniRef:UniRef90_C4XE91" ], "nt": "ATGGAAAAAACAGTAATTTCAAGTGTAAGTAATCCTAAAATTAAGTATTTAAAAGAGGTTCAAAAAGACAATAAAAGTGATTACTTTTTAGTATCTGGTTACCATTTAGTACAAGAAGCTTTAGCTAAAAATTTAGTTATTGAAATTTTTGAAGAAGAAAGCAAAAGCACCTATAAAACAGCTACTTTGGTAAAAAGTAATGTTTTAAAAAGTATCTCAAGTACAATGCATCCAGATGGTGTTGTAGCCTTGTGTTTAAAACCTAAAAGTCAAATAAAAAATAAAGTCATTTTGCTTGATAATGTCCAAGATCCAGGTAATGTTGGCACTATTATTAGAAATGCTGCATGCTTTGGTTTTGATACGGTTATTTCGAATGTAAATTTTTATAATGACAAAGTAGTTAGAGCAACTCAAGGTGCACTTTTCCAAGTTTCATTAATCAAAGAAAACAATAATGAAGATTTAATTAAAGTACTAAATAATGAAGGTTATTTTATTGTTGCCACTTCTCTCGATAAAGATTCAATTAAACTAGATGAATTAAAAATAAATAAAGAAAAAGTGGTTCTAATTTTAGGAAATGAAGGCAACGGAATCAATAAAAATTTATATAAATATTGTGACCAAAAAGTGTACATCCCTATTGAATTTGAAAGCCTAAATGTTGCGGTAGCAAGTGGAATAATTTTAAATAAAATATATAATAAATAA", "aa": "MEKTVISSVSNPKIKYLKEVQKDNKSDYFLVSGYHLVQEALAKNLVIEIFEEESKSTYKTATLVKSNVLKSISSTMHPDGVVALCLKPKSQIKNKVILLDNVQDPGNVGTIIRNAACFGFDTVISNVNFYNDKVVRATQGALFQVSLIKENNNEDLIKVLNNEGYFIVATSLDKDSIKLDELKINKEKVVLILGNEGNGINKNLYKYCDQKVYIPIEFESLNVAVASGIILNKIYNK", "aa_hexdigest": "49962aab1b0023aa589850a65d2a39c5", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI0001E32F90", "ncbi_nrp_id": "WP_013354390.1", "uniref100_id": "UniRef100_C4XE91", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F90", "RefSeq:WP_013354390.1", "UniRef:UniRef100_C4XE91" ] }, "ips": { "uniref100_id": "UniRef100_C4XE91", "uniref90_id": "UniRef90_C4XE91" }, "psc": { "uniref90_id": "UniRef90_C4XE91", "product": "SpoU rRNA Methylase family", "uniref50_id": "UniRef50_A0A3P8KX10" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KX10", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KX10" ], "product": "tRNA/rRNA methylase" }, "genes": [ "spoU" ], "id": "OAIKOHHJFB_401", "locus": "GCA000209735_00371" }, { "type": "cds", "sequence": "contig_1", "start": 206959, "stop": 207060, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAACAAATAACGAAAAATTTTTCAACAAAAATTTTAGTAGAGAATTAAATGGTTACTCAGTTCCTGAAGTAGATAATTTTATTGATCAATTGTGA", "aa": "MKKTNNEKFFNKNFSRELNGYSVPEVDNFIDQL", "aa_hexdigest": "8611b7902e270cc7485b45ba406ab0c1", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3967.376000000001, "isoelectric_point": 6.038369941711425 }, "id": "OAIKOHHJFB_402", "locus": "GCA000209735_00372" }, { "type": "cds", "sequence": "contig_1", "start": 207257, "stop": 207475, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAAGCCATGCGTGAAATTAAGGAAAATGCAACCTTAGCCGATATCTTTTTAATTGTTCTAGATGCTAGAATTCCAATTAGCAGTTATAATGAAGATTTTGATAATATCGCGCCTCAAAAACCAAGACTTTTTGTAATCACAAAAAGTGATTTAATGGATAAAAGTAAAAAAGAAAAGATCCAAAAAAGATTTCAAAATGAAAAACTTTTGTGA", "aa": "MAKAMREIKENATLADIFLIVLDARIPISSYNEDFDNIAPQKPRLFVITKSDLMDKSKKEKIQKRFQNEKLL", "aa_hexdigest": "8eca640255274886bdac0eb287ae0541", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8410.785899999999, "isoelectric_point": 9.345675468444824 }, "id": "OAIKOHHJFB_403", "locus": "GCA000209735_00373" }, { "type": "cds", "sequence": "contig_1", "start": 207728, "stop": 208033, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATACACCTGGAATTTTATTGCCTAAACATGAAGATCAAAGTGCAGCAATTAAATTAGCAATGATAGGGTCAATTGAAACAAATATTTTTCCTCAAAGATTTTTAGCAGAAAACTTTTTAAAAGTTATTGTTAAATATTATCCAAACAAAATTAAAGAAGAATTCAATATTGATATTGAATCAAAGGATGATTTGAGCGAAACTGAAGCTTATAACATAGTTGCTCAAATTGCTGATAATAAAAACTTTAAAGTTGATAAAAAAATAGATTTTGACCGAACATATAAATATATATTGAATTGA", "aa": "MDTPGILLPKHEDQSAAIKLAMIGSIETNIFPQRFLAENFLKVIVKYYPNKIKEEFNIDIESKDDLSETEAYNIVAQIADNKNFKVDKKIDFDRTYKYILN", "aa_hexdigest": "4bd537d463d1bbe23bd18d8bac485910", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11745.310899999982, "isoelectric_point": 5.1215002059936525 }, "id": "OAIKOHHJFB_404", "locus": "GCA000209735_00374" }, { "type": "cds", "sequence": "contig_1", "start": 208076, "stop": 208264, "strand": "+", "frame": 2, "gene": null, "product": "Phage protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE94", "UniRef:UniRef90_C4XE94" ], "nt": "ATGGATCAAGAAATAAAAAACGAAAAGAAAAAATATTCTTTTGAAGAAAAAGTAGAAGCTTATAAAAAAGTGTACAAAAGCAATTTAGATCATTTGAATCTTAGAAATCAAATGAATATTAAAGCTTTTGGACTCCTATTTATTTTTATGATAATTCTATTGATCATAACTGTAATCGCTTATGCTTGA", "aa": "MDQEIKNEKKKYSFEEKVEAYKKVYKSNLDHLNLRNQMNIKAFGLLFIFMIILLIITVIAYA", "aa_hexdigest": "d1022672db1a9e878e1e3842c3b10eff", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 208076, "stop": 208507, "observations": { "mutation": [ 208262, 208307 ], "stop": [ 208262, 208307 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE94", "query_cov": 1.0, "subject_cov": 0.43356643356643354, "identity": 1.0, "score": 111.0, "evalue": 4.72e-30, "gene_start": 1, "gene_end": 62, "reference_start": 1, "reference_end": 62, "reference_sequence": "MDQEIKNEKKKYSFEEKVEAYKKVYKSNLDHLNLRNQMNIKAFGLLFIFMIILLIITVIAYAWQNKAAPSITYTTLLWILICVFSILTILSLYLLILFFIEYSLIKKIGLKKSEQEIEASIRKFVKFGFKKYPKKQMEMLEKF" }, "paralog": false, "identity": 0.986013986013986, "score": 269.0, "evalue": 7.39e-95, "description": "Internal stop codon at 208262, 208307. Nonsense mutation around 208262, 208307.", "psc": { "uniref90_id": "UniRef90_C4XE94", "product": "Phage protein", "uniref50_id": "UniRef50_C4XE94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE94", "UniRef:UniRef50_C4XE94" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE94" ], "product": "Phage protein" } }, "id": "OAIKOHHJFB_405", "locus": "GCA000209735_00375" }, { "type": "cds", "sequence": "contig_1", "start": 208633, "stop": 208866, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter related protein", "db_xrefs": [ "RefSeq:WP_232048437.1", "SO:0001217", "UniParc:UPI000301A973", "UniRef:UniRef100_UPI0001E32F93", "UniRef:UniRef50_D1J8M4", "UniRef:UniRef90_A0AB32XAQ9" ], "nt": "ATGCCAAAACTAACTGAAACTCAAGAAACAAGAATGAGCAACTCAATTAAAGAATTCGTTGAAAATCTTGAAAAACATGAAAACAGAATTTTAAATCACCCTGCAATTGAACCTAGAGAATATGATGGACTAGAAAAGCTTATAGAAATAAAAGGCCTATGTAAAGAATATAAAATTAAAGGCAAAAAATTTAGAGCCGTTGATGATATGAACTTAACATTGTACAAAAGTTAA", "aa": "MPKLTETQETRMSNSIKEFVENLEKHENRILNHPAIEPREYDGLEKLIEIKGLCKEYKIKGKKFRAVDDMNLTLYKS", "aa_hexdigest": "3aa1817c577c0b6e9995348bb9c32f82", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000301A973", "ncbi_nrp_id": "WP_232048437.1", "uniref100_id": "UniRef100_UPI0001E32F93", "db_xrefs": [ "SO:0001217", "UniParc:UPI000301A973", "RefSeq:WP_232048437.1", "UniRef:UniRef100_UPI0001E32F93" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E32F93", "uniref90_id": "UniRef90_A0AB32XAQ9" }, "psc": { "uniref90_id": "UniRef90_A0AB32XAQ9", "product": "ABC transporter related protein", "uniref50_id": "UniRef50_D1J8M4" }, "pscc": { "uniref50_id": "UniRef50_D1J8M4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M4" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "OAIKOHHJFB_406", "locus": "GCA000209735_00376" }, { "type": "cds", "sequence": "contig_1", "start": 208918, "stop": 209484, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE96", "UniRef:UniRef90_C4XE96" ], "nt": "ATGCTTGTTGGTATTCTTAAACCAAGTGCAGGAAATATTAAATACTTTTTTGGCGACTCAATGGATAAAGGAAAAATTGATGCAACAAAAATTGGAATTCAATTTCAAGATTCCTCATATCCACAAGGGTTAACTGTTCGTGATGTAATTAAATCAATGAACAAAATCTATGGTAACAAAATGAAAGGTTTAGAGCTAGATTATTTAATTAAAATTTTTGGTGTTGATGAATATATAGATAATAAAGCAGCTTCACTAAGTGGTGGTCAACATCAAAGATTAAATGCTCTTTTAGCTTTAATTCATAAACCTGAATTAGTTATCTTAGATGAATTAAGTACTGGATTGGATATTAAAATTAAGACCAGACTTGTTGAATTTATTAACAATTTTGTTAAGGATAATAAATCAACTTTATTGATTGTTTCACATGATATTAATGAAATTAATACACTAGCAGATCGTATTGTAATTATGAACAAAGGCAAAGTTATTTTCGATAAAACCAAAGACGAAGCAGTAAAAGAATTCAAGACTCTGGCAGCTTGCTTAGAGTACTACATTTAA", "aa": "MLVGILKPSAGNIKYFFGDSMDKGKIDATKIGIQFQDSSYPQGLTVRDVIKSMNKIYGNKMKGLELDYLIKIFGVDEYIDNKAASLSGGQHQRLNALLALIHKPELVILDELSTGLDIKIKTRLVEFINNFVKDNKSTLLIVSHDINEINTLADRIVIMNKGKVIFDKTKDEAVKEFKTLAACLEYYI", "aa_hexdigest": "1b2fae4c9dbc52059fe1f75e53f6fca3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XE96", "query_cov": 1.0, "subject_cov": 0.9215686274509803, "identity": 1.0, "score": 367.0, "evalue": 4.58e-128, "valid": true, "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_C4XE96" }, "pscc": { "uniref50_id": "UniRef50_C4XE96", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE96" ], "product": "ABC transporter domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_407", "locus": "GCA000209735_00377" }, { "type": "cds", "sequence": "contig_1", "start": 209493, "stop": 209627, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGATAAAGAAAAATTAAATGAAACAAACAATAATCGAAAAAACATCAAAAAACAAAAAAGAATTATGCAAAATTCTAAACAAATGAATTCTTTTTTAGGTGTTTTTAGTATTATTAATACACACTTTTGA", "aa": "MEDKEKLNETNNNRKNIKKQKRIMQNSKQMNSFLGVFSIINTHF", "aa_hexdigest": "524f818323dd96e7ee2b338dfc14b92b", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5298.046899999999, "isoelectric_point": 10.118586921691893 }, "id": "OAIKOHHJFB_408", "locus": "GCA000209735_00378" }, { "type": "cds", "sequence": "contig_1", "start": 209709, "stop": 209897, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTAGGTTCTTCTATTACAATAGGACCTGTGGCTGTAGCTTGTGTTTCACTTCCTACTGCAATCTTCGAATTTAAAAAATCAACATTGCTTAAGAGAATTGGGGCTTCTAATATAAAACCGCTAGCTTTCTTAAGTTATACGGCCTTTTATTACTTCTTTGTTATGGTTCTTTCTGCTATTTGA", "aa": "MILGSSITIGPVAVACVSLPTAIFEFKKSTLLKRIGASNIKPLAFLSYTAFYYFFVMVLSAI", "aa_hexdigest": "9101cc85ce64dfdd67f680e611eaccea", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6722.067, "isoelectric_point": 9.655059242248537 }, "id": "OAIKOHHJFB_409", "locus": "GCA000209735_00379" }, { "type": "cds", "sequence": "contig_1", "start": 209916, "stop": 210035, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTTGGACCTCAATATTTTAATGAAGGTAAAGTATTATTTGAAACAACATTTGGTTCACAAACTATAGCTGTTAGAACAACTCCAATGAAAGACTTATTTACAAGAATTCATTGA", "aa": "MVFGPQYFNEGKVLFETTFGSQTIAVRTTPMKDLFTRIH", "aa_hexdigest": "89a559746d51c1689a36966f10a29b8c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4509.1675, "isoelectric_point": 8.274984550476077 }, "id": "OAIKOHHJFB_410", "locus": "GCA000209735_00380" }, { "type": "cds", "sequence": "contig_1", "start": 210270, "stop": 211079, "strand": "+", "frame": 3, "gene": null, "product": "ABC-2 type transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE97", "UniRef:UniRef50_UPI000FF82EAB", "UniRef:UniRef90_C4XE97", "UniRef:UniRef90_UPI000FF82EAB" ], "nt": "ATGGCTTTTAGTGGTAGTGCATATTTAAGCTCATTATATGATGGTTATAACTTTACTGCTGGAGCTGCTGAAAAAATTTATGCTTTAAACAATTTGCAAACATTTTTGGAAACAATAGCTAAAAATACGGAAACAAATATAATAACAGCAACACAAATTCATTCTAAAGGCAGTGCACTTTTTCCTGAATCTGCTCAAATAAAAGCATATATTGATGAAATAGCAAAATCAATAAAAATGATAATGTTTAATTTGACGGATCATGCTATAAATAAGGATGATGCAATTAATTTAACTATTCGTTATAATTCATATAATATCTTTAATGCAACAGAAGTTTACAAATCGATTAATGCTTTAAAAACTTATGAAGGAACTAACTTAACATTTGATGAAGCTGTTAAAAACTCAATTTATGAAATTTTGAAAAAAGCAGTAAACGAAAATGAAAATGATAATTATATTAAGCTTTTAAATGGTATGTCAAATCAAGATTTATGTCCAAATATTCAAGATTATCCATATAAAGTAATAAATGGTAAATCTATTGAAAAAGTAAAAGACCTTGATTTAAATAAATTCATTGATGATTATCGTAAAGAAATGAATGAAAGTATGAAACAATCAATGTTAGCTGTAAAAACAAATGAAATTTACAATCTAACAATGAATAATATTAAAACCAATTATAAAAATTTTATTGATACAAATAAAGCTTATGTTCAATTATCTCAAGGTGGTATTAATAAAGATTCGATTTTAGAAATTGGCAGTGTTGTTGAAAACTATGTTAACTTTATTCTTCCATGA", "aa": "MAFSGSAYLSSLYDGYNFTAGAAEKIYALNNLQTFLETIAKNTETNIITATQIHSKGSALFPESAQIKAYIDEIAKSIKMIMFNLTDHAINKDDAINLTIRYNSYNIFNATEVYKSINALKTYEGTNLTFDEAVKNSIYEILKKAVNENENDNYIKLLNGMSNQDLCPNIQDYPYKVINGKSIEKVKDLDLNKFIDDYRKEMNESMKQSMLAVKTNEIYNLTMNNIKTNYKNFIDTNKAYVQLSQGGINKDSILEIGSVVENYVNFILP", "aa_hexdigest": "7cffe5bea967eec53048794c8ac67e56", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000FF82EAB", "query_cov": 0.9330855018587361, "subject_cov": 0.9061371841155235, "identity": 1.0, "score": 480.0, "evalue": 1.83e-170, "valid": true, "uniref50_id": "UniRef50_UPI000FF82EAB" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF82EAB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF82EAB" ] }, "genes": [], "truncated": "both", "pseudogene": { "start": 209970, "stop": 211139, "observations": { "mutation": [ 210033, 210219, 211077, 211083, 211125 ], "stop": [ 210033, 210219, 211077, 211083, 211125 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE97", "query_cov": 1.0, "subject_cov": 0.4908759124087591, "identity": 1.0, "score": 515.0, "evalue": 5.12e-180, "gene_start": 1, "gene_end": 269, "reference_start": 260, "reference_end": 528, "reference_sequence": "MEDKEKLNETNNNRKNIKKQKRIMQNSKQMNSFLGVFSIINTHFWKAFVGPFFAFGYPIVFVIILGAVFNYEMILGSSITIGPVAVACVSLPTAIFEFKKSTLLKRIGASNIKPLAFLSYTAFYYFFVMVLSAIWTALVALMVFGPQYFNEGKVLFETTFGSQTIAVRTTPMKDLFTRIHWAGYIYSAIVLTLVSIAVGLFIVSVSKSILTIQAIGSSLLIISMFLTGQVLPISQIIGQKVMWYLSYITPFKSPITQNTMAFSGSAYLSSLYDGYNFTAGAAEKIYALNNLQTFLETIAKNTETNIITATQIHSKGSALFPESAQIKAYIDEIAKSIKMIMFNLTDHAINKDDAINLTIRYNSYNIFNATEVYKSINALKTYEGTNLTFDEAVKNSIYEILKKAVNENENDNYIKLLNGMSNQDLCPNIQDYPYKVINGKSIEKVKDLDLNKFIDDYRKEMNESMKQSMLAVKTNEIYNLTMNNIKTNYKNFIDTNKAYVQLSQGGINKDSILEIGSVVENYVNFILPWLWIVVLVGLSQKSFTWNTR" }, "paralog": false, "identity": 0.987146529562982, "score": 736.0, "evalue": 1.8e-269, "description": "Internal stop codon at 210033, 210219, 211077, 211083, 211125. Nonsense mutation around 210033, 210219, 211077, 211083, 211125.", "psc": { "uniref90_id": "UniRef90_C4XE97", "product": "ABC-2 type transporter domain-containing protein", "uniref50_id": "UniRef50_C4XE97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE97", "UniRef:UniRef50_C4XE97" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE97" ], "product": "ABC-2 type transporter domain-containing protein" } }, "id": "OAIKOHHJFB_411", "locus": "GCA000209735_00381" }, { "type": "cds", "sequence": "contig_1", "start": 211241, "stop": 211411, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE98", "UniRef:UniRef90_C4XE98" ], "nt": "TTGTTTAAAAAATATAAACTTGAAATTCAAATAGATTTAACTAATGATAAAGAAGATCTTGAAAAAAGAAACCATACAACAGCTAATAAATATATGGATTTAGTCTATAAAGTCAAAATTGCTGAAAAAAAGATTAAAGAAGGTTTTGCTGCATATTTAAAAATGCTATGA", "aa": "MFKKYKLEIQIDLTNDKEDLEKRNHTTANKYMDLVYKVKIAEKKIKEGFAAYLKML", "aa_hexdigest": "c98de4fdb68c65f42dc564070b0ffdba", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 211232, "stop": 211498, "observations": { "mutation": [ 211243, 211444 ], "stop": [ 211243, 211444 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE98", "query_cov": 1.0, "subject_cov": 0.6363636363636364, "identity": 0.982, "score": 108.0, "evalue": 8.74e-30, "gene_start": 1, "gene_end": 56, "reference_start": 30, "reference_end": 85, "reference_sequence": "MYNNKKVFPFIKQEFNENWKLLTKEIVQNLFKKYKLEIQIDLTNDKEDLEKRNHTTANKYMDLVYKVKIAEKKIKEGFAAYLKMLWNV" }, "paralog": false, "identity": 0.9659090909090909, "score": 169.0, "evalue": 2.05e-56, "description": "Internal stop codon at 211243, 211444. Nonsense mutation around 211243, 211444.", "psc": { "uniref90_id": "UniRef90_C4XE98", "uniref50_id": "UniRef50_C4XE98", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE98", "UniRef:UniRef50_C4XE98" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE98", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE98" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6740.905500000001, "isoelectric_point": 9.363597679138184 }, "id": "OAIKOHHJFB_412", "locus": "GCA000209735_00382" }, { "type": "cds", "sequence": "contig_1", "start": 211511, "stop": 211627, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59E84", "UniRef:UniRef100_A0AB32XB43", "UniRef:UniRef50_A0AB32XB43", "UniRef:UniRef90_A0AB32XB43" ], "nt": "ATGAATAGTGCATTAAGTACTTTAGCAACCTTACTTTTAACCAGTGCTTCATGCATTAAAGAAGACAGAATGTATGACAAGCCTGCTAAAGAAATTGATAAATATAAGCAGCTTTAA", "aa": "MNSALSTLATLLLTSASCIKEDRMYDKPAKEIDKYKQL", "aa_hexdigest": "ed80be0d1b049de55233c5bf81ab5965", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001F59E84", "uniref100_id": "UniRef100_A0AB32XB43", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59E84", "UniRef:UniRef100_A0AB32XB43" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XB43", "uniref90_id": "UniRef90_A0AB32XB43" }, "psc": { "uniref90_id": "UniRef90_A0AB32XB43", "uniref50_id": "UniRef50_A0AB32XB43" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XB43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XB43" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4290.974300000002, "isoelectric_point": 7.869091606140137 }, "id": "OAIKOHHJFB_413", "locus": "GCA000209735_00383" }, { "type": "cds", "sequence": "contig_1", "start": 211748, "stop": 212455, "strand": "+", "frame": 2, "gene": null, "product": "Uridine phosphorylase", "db_xrefs": [ "EC:2.4.2.1", "KEGG:K03784", "RefSeq:WP_013526694.1", "SO:0001217", "UniParc:UPI0001F59E85", "UniRef:UniRef100_C4XE99", "UniRef:UniRef50_C5J6Z6", "UniRef:UniRef90_C4XE99" ], "nt": "ATGCCAACACCACATATTAGTGCGCAAAAAGGTGAAATAGCTAAAATTGTATTAATGCCAGGAGATCCCTTAAGAGCAGAATACATGGCTAAAAAATTTTTAAAGAATCCTAAATTAGTTTCAAGCGTTAGAAATGTTTTATTTTATACAGGTAAATATAATGGCAAAGATGTAACAATAGGGGCTAGTGGTATGGGTTCAGCTTCAATAGGAATTTATGCTTATGAGCTTTTTACTGTTTATGAAGTTGAAACAATAGTTAGAGTTGGTTCAACAGGTTCATATATCAAAGAATTAAATGTTAAGCAACCTGTGTTAGTTAAAAGAGCTTATGCTGATGGAACAGGCTTTGTTGAATTAATGGTTGGAAATAAAAAACAGGAAAATTATCCTGATTTGGAAACTTATGAAAAGCTTAAACAAACAACCAAAGAAATGAATGTTGAATTGAAAGAAGTTTCTTGCCATTCAACTGATGTTTTTTATGGATTAAGATCACTAGAGGATACTATAAAAATAACTAAATGTCAAACTGTTGATAATGAATGTTTTGCTCTTTTTGCAACAGCACAAAGATGCAAGAAAAAAGCAGCTGCATTATTAAGTGTTTCTGAAAATATTGTAACTGGAGCGTCATTAAGTTCAGACGAAAGACTCAAAGAATTTAGTACAATGTTTTTAGTAGCCTTAAATGCTTTAACTAAATAA", "aa": "MPTPHISAQKGEIAKIVLMPGDPLRAEYMAKKFLKNPKLVSSVRNVLFYTGKYNGKDVTIGASGMGSASIGIYAYELFTVYEVETIVRVGSTGSYIKELNVKQPVLVKRAYADGTGFVELMVGNKKQENYPDLETYEKLKQTTKEMNVELKEVSCHSTDVFYGLRSLEDTIKITKCQTVDNECFALFATAQRCKKKAAALLSVSENIVTGASLSSDERLKEFSTMFLVALNALTK", "aa_hexdigest": "2052ad9099d0a63fd2ed41041f4d0e6f", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001F59E85", "ncbi_nrp_id": "WP_013526694.1", "uniref100_id": "UniRef100_C4XE99", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59E85", "RefSeq:WP_013526694.1", "UniRef:UniRef100_C4XE99" ] }, "ips": { "uniref100_id": "UniRef100_C4XE99", "uniref90_id": "UniRef90_C4XE99" }, "psc": { "uniref90_id": "UniRef90_C4XE99", "product": "Uridine phosphorylase", "ec_ids": [ "2.4.2.1" ], "uniref50_id": "UniRef50_C5J6Z6", "kegg_orthology_id": "K03784" }, "pscc": { "uniref50_id": "UniRef50_C5J6Z6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C5J6Z6" ], "product": "Uridine phosphorylase" }, "genes": [], "id": "OAIKOHHJFB_414", "locus": "GCA000209735_00384" }, { "type": "cds", "sequence": "contig_1", "start": 212466, "stop": 212975, "strand": "+", "frame": 3, "gene": "tpx", "product": "thiol peroxidase", "db_xrefs": [ "EC:1.11.1.-", "EC:1.11.1.24", "GO:0008379", "GO:0016684", "KEGG:K11065", "RefSeq:WP_013354398.1", "SO:0001217", "UniParc:UPI0001A8C0CB", "UniRef:UniRef100_C4XEA0", "UniRef:UniRef50_A8MM68", "UniRef:UniRef90_C4XEA0" ], "nt": "ATGAATAAAAGACAAGAAACAACTTTTTTCAAAAATAAAGTTACTTTATTAGGTGATAAAATTGAAGTAGGTCAAAAAGCACCAAATTTTACAGCCTTAAATAATGAATTAAAATCTGTTCAATTAAAAGATTTTAAAGATAAAGTAATATTAATCTCATCTGTTCCTTCTTTAGACACTGGAGTTTGTCAAATGCAAACTGTAAGATTTAACAAAGAAGTTGAACAAATTAAAGGGCTAAAAGTAATTACAATTAGTTGTGATTTGCCTTTTGCTCAAAGTAGATTTTGTACTTCATTTAACACTAATAATGTTGTTACTTTATCAGATCATAAAAATTTAGATTTTGGTCTTAAATACGGATGTGTAATAGAAGAATTAAGGCTCTTAAATAGAGCTATATTTGTAATTGATAAAAATGGCATAATTCAATATGTAGAATATGTTAAAGAAAACACAGATCATCCTAATTATGATAAAGCTTTAAAATGTGTTAAAAATATAATTTAG", "aa": "MNKRQETTFFKNKVTLLGDKIEVGQKAPNFTALNNELKSVQLKDFKDKVILISSVPSLDTGVCQMQTVRFNKEVEQIKGLKVITISCDLPFAQSRFCTSFNTNNVVTLSDHKNLDFGLKYGCVIEELRLLNRAIFVIDKNGIIQYVEYVKENTDHPNYDKALKCVKNII", "aa_hexdigest": "2cd7c50fc38f1dd5528e8b82e42a6e94", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C0CB", "ncbi_nrp_id": "WP_013354398.1", "uniref100_id": "UniRef100_C4XEA0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C0CB", "RefSeq:WP_013354398.1", "UniRef:UniRef100_C4XEA0" ] }, "ips": { "uniref100_id": "UniRef100_C4XEA0", "uniref90_id": "UniRef90_C4XEA0" }, "psc": { "uniref90_id": "UniRef90_C4XEA0", "gene": "tpx", "product": "thiol peroxidase", "ec_ids": [ "1.11.1.-", "1.11.1.24" ], "uniref50_id": "UniRef50_A8MM68", "kegg_orthology_id": "K11065", "go_ids": [ "GO:0008379", "GO:0016684" ] }, "pscc": { "uniref50_id": "UniRef50_A8MM68", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A8MM68" ], "product": "Thiol peroxidase" }, "genes": [ "tpx" ], "id": "OAIKOHHJFB_415", "locus": "GCA000209735_00385" }, { "type": "cds", "sequence": "contig_1", "start": 213321, "stop": 213554, "strand": "+", "frame": 3, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEA1", "UniRef:UniRef90_C4XEA1" ], "nt": "ATGAAAAAATTATATTTTGGACTTGTTGGCGCTAGTGCCTTATTGCCAGTAATGACAGTTGTTTCGTGTAGTAACGAAGACAAGAAACTTCATAATGAAGCACAAAAATTATATGAAGAATATAAAACTAAGAAAGAAAAATTATACAAAGCTAAACTTAATGCAAATGAAATTACTCAAAGCTTGTATGATGATGTTGTAAACTCATCAAGACACAAAGATGTTAAAGACTGA", "aa": "MKKLYFGLVGASALLPVMTVVSCSNEDKKLHNEAQKLYEEYKTKKEKLYKAKLNANEITQSLYDDVVNSSRHKDVKD", "aa_hexdigest": "28275387d2515573e29c00381c066fbf", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 213318, "stop": 213671, "observations": { "mutation": [ 213552, 213579, 213639 ], "stop": [ 213552, 213579, 213639 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEA1", "query_cov": 1.0, "subject_cov": 0.6581196581196581, "identity": 1.0, "score": 147.0, "evalue": 2.01e-44, "gene_start": 1, "gene_end": 77, "reference_start": 2, "reference_end": 78, "reference_sequence": "MMKKLYFGLVGASALLPVMTVVSCSNEDKKLHNEAQKLYEEYKTKKEKLYKAKLNANEITQSLYDDVVNSSRHKDVKDWAKEFLSKDWDKNKNTKKVIIFNLKRFLKWTTEELNKYQ" }, "paralog": false, "identity": 0.9658119658119658, "score": 221.0, "evalue": 5.15e-76, "description": "Internal stop codon at 213552, 213579, 213639. Nonsense mutation around 213552, 213579, 213639.", "psc": { "uniref90_id": "UniRef90_C4XEA1", "product": "Lipoprotein", "uniref50_id": "UniRef50_C4XEA1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEA1", "UniRef:UniRef50_C4XEA1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEA1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEA1" ], "product": "Lipoprotein" } }, "id": "OAIKOHHJFB_416", "locus": "GCA000209735_00386" }, { "type": "cds", "sequence": "contig_1", "start": 213717, "stop": 213926, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTTTATCACTATTTTTGGATTTTTAACATCATTAATTAACTATGACAAATATGGTATTATAGGCTCATTTATGTATATTGCATTATTTGTTTCATTCTTAACATATTTTATGTTTTTAGGAATTATAGTTAATGAAGCATATTACAAAACTTATGTGTCTTCATACACTGTTTCTAAACGTAGTATATTTGGCAAAAAATTTTAA", "aa": "MIFITIFGFLTSLINYDKYGIIGSFMYIALFVSFLTYFMFLGIIVNEAYYKTYVSSYTVSKRSIFGKKF", "aa_hexdigest": "716b9c63099d5cc5a312984a991830d6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8094.541599999997, "isoelectric_point": 9.477190971374515 }, "id": "OAIKOHHJFB_417", "locus": "GCA000209735_00387" }, { "type": "cds", "sequence": "contig_1", "start": 214269, "stop": 214433, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGTTTTACTTTCGTTAATTCCTGGTTATCATACTTTATTTATTAATGAAGTTTTAACTCGAATTATTCCTGGTGTTACTTCTATTATTAAGATCCCTGACACCACAAAAAAAGGCGCTCAATTAACTTCTTTAATTATTCTTTGTTTGGCTTCTTTATGA", "aa": "MIVLLSLIPGYHTLFINEVLTRIIPGVTSIIKIPDTTKKGAQLTSLIILCLASL", "aa_hexdigest": "f908270ace9a5d457ffeac582491fca2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5847.109199999999, "isoelectric_point": 9.10185604095459 }, "id": "OAIKOHHJFB_418", "locus": "GCA000209735_00388" }, { "type": "cds", "sequence": "contig_1", "start": 214816, "stop": 215220, "strand": "-", "frame": 2, "gene": "engA", "product": "GTP-binding protein EngA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0QDV4" ], "nt": "ATGGTTGAATTCGAAAAGAAAATGCGTAACAAATTTCATTTTGTACCTTGGGTTCCTATTTGTTTTGTTTCAGTTAAAACAAACTCAAGAATGGACAAATTAATTGACACAATAATTAAAGTTAAACAAAATCTTGAACGTGATGTTAAACCTTCAGTTCTTTCTAACTTAATCAGAGAAACACAAATGATTCAGCCAGCTCCTCCTTATAATGGTGGCCGTTTAAATATTTATTTTGCTCGTAAAACTCAAGATCCAGTTCCTACTTTTGTTTTCTTTGTTAACAATAAAAAATATGTCCACTTTAGCTATGAAAGATTTTTAGAAAAGCAAATTAGACAAGTTTTAGACTACACTGGTTGTCCAATTAATTTAGTTTTCAAAAACAAAAGTGGTTTAGAATAA", "aa": "MVEFEKKMRNKFHFVPWVPICFVSVKTNSRMDKLIDTIIKVKQNLERDVKPSVLSNLIRETQMIQPAPPYNGGRLNIYFARKTQDPVPTFVFFVNNKKYVHFSYERFLEKQIRQVLDYTGCPINLVFKNKSGLE", "aa_hexdigest": "368c090a2b6a3155c58b38da933c87c6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0QDV4", "query_cov": 0.8432835820895522, "subject_cov": 0.9912280701754386, "identity": 0.6459999999999999, "score": 159.0, "evalue": 3.45e-48, "valid": false, "product": "GTP-binding protein EngA", "uniref50_id": "UniRef50_A0A3B0QDV4" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0QDV4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0QDV4" ], "product": "GTP-binding protein EngA" }, "genes": [ "engA" ], "id": "OAIKOHHJFB_419", "locus": "GCA000209735_00389" }, { "type": "cds", "sequence": "contig_1", "start": 215248, "stop": 215718, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00350.29", "PFAM:PF02421.24" ], "nt": "GTGGATCATATTTTTCCTATTTCAGCTCAACATGGTAATGGTGTTGGTGATGTCTTAGACCAATGTCTTAAACATCTTAGACTTGATGATGAAAAGAGTGACAAACTATTCAAACTTTCAATTATTGGACGTCCTAATTCAGGTAAAAGTTCAATGTTAAATAGATTAGCAAATGAAAACCGTTCAATAGTTTCTGATATTGCGGGAACTACTAGAGATAGTGTTAAAAGTATTGTAAAAATCAAAGATGAAGTTTTTGAAATCATTGATACAGCTGGTATTACTAGAAAAAGTAAAATTGAAGATAGCGTTGACCATTATGCTTTAAAACGCGCTATGAGTTCACTTGACGAATCTAACTTATCTTTAGTTTTAATTGACTCAACTCAAGAATTATCACACTTCGATTCTAGAATTATAGGCTATGCTTTAGACAACAATAAACCAATAATTATTGTGGTTAATAAATGA", "aa": "MDHIFPISAQHGNGVGDVLDQCLKHLRLDDEKSDKLFKLSIIGRPNSGKSSMLNRLANENRSIVSDIAGTTRDSVKSIVKIKDEVFEIIDTAGITRKSKIEDSVDHYALKRAMSSLDESNLSLVLIDSTQELSHFDSRIIGYALDNNKPIIIVVNK", "aa_hexdigest": "0d3731e8754831daa3dbc867dd11e066", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00350.29", "name": "Dynamin_N", "length": 78, "aa_cov": 0.5, "hmm_cov": 0.9102564102564102, "evalue": 4.008259004605064e-09, "score": 32.799156188964844, "start": 78, "stop": 155 }, { "id": "PF02421.24", "name": "FeoB_N", "length": 118, "aa_cov": 0.7564102564102564, "hmm_cov": 0.9491525423728814, "evalue": 3.6734084363883805e-10, "score": 35.63899230957031, "start": 38, "stop": 155 } ], "seq_stats": { "molecular_weight": 17318.51749999999, "isoelectric_point": 6.193256187438965 }, "id": "OAIKOHHJFB_420", "locus": "GCA000209735_00390" }, { "type": "cds", "sequence": "contig_1", "start": 215731, "stop": 215859, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGATGGTGCTTCTGACATCACTAATGACGATTTAATGATTTACAGTATGCTTCAAAAATCTGGCAAACCTATTGTCGTAGTTGCAAACAAATTAGATAATATTTCACAGTTTAATTATTCATGA", "aa": "MVDGASDITNDDLMIYSMLQKSGKPIVVVANKLDNISQFNYS", "aa_hexdigest": "20814215acf8a8b0796e5cd1a8cbeede", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4636.2374, "isoelectric_point": 4.282727241516113 }, "id": "OAIKOHHJFB_421", "locus": "GCA000209735_00391" }, { "type": "cds", "sequence": "contig_1", "start": 215980, "stop": 216123, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02421.24" ], "nt": "ATGAGAAACAACGTTATAGCCATTATTGGAAAACCAAATGTTGGTAAAAGTACCCTATTTAATAGGCTAATTGGTAAAAGAAGTTCAATAATTTATGACCGTCCTGGAGTAACAAGGGACCGTCTTTATGAAACCTTTTCTTGA", "aa": "MRNNVIAIIGKPNVGKSTLFNRLIGKRSSIIYDRPGVTRDRLYETFS", "aa_hexdigest": "34ef83a718c1b72f31f2c9c8bf5c3e78", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3S4P1B8", "query_cov": 0.8297872340425532, "subject_cov": 0.8297872340425532, "identity": 0.615, "score": 56.6, "evalue": 8.25e-10, "valid": false, "product": "GTP-binding protein EngA", "uniref50_id": "UniRef50_A0A4Z0KSI3" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02421.24", "name": "FeoB_N", "length": 37, "aa_cov": 0.7872340425531915, "hmm_cov": 0.972972972972973, "evalue": 5.4119344013552565e-09, "score": 31.843690872192383, "start": 5, "stop": 41 } ], "seq_stats": { "molecular_weight": 5368.181400000001, "isoelectric_point": 10.956482505798338 }, "id": "OAIKOHHJFB_422", "locus": "GCA000209735_00392" }, { "type": "cds", "sequence": "contig_1", "start": 216127, "stop": 216435, "strand": "-", "frame": 2, "gene": "cmk", "product": "(d)CMP kinase", "db_xrefs": [ "EC:2.7.4.25", "GO:0004127", "GO:0005524", "GO:0015949", "SO:0001217", "UniRef:UniRef50_A5IZG6", "UniRef:UniRef90_C4XEA4" ], "nt": "ATGGATGGAAGAGATACTACATTCAAAATTATGCCTTATGCAGAATTAAAAATCTTTTTAACAGCAACACCAGAAGAAAGAGCAATGAGAAGATTAAACCAAAACATTGCTTTAGGATTTAATATGGACTACAACAAAGTTCTTGAAGAAGTTAAATTAAGAGACTATCAAGACATGAATCGTGAAGTAGACCCTCTTCATAAAGTTAAAGAAGCAAAAGAAATAGACTGTACAAATATGGGTTTTGAAGCAGTTGTTGTTGAAATTATTGAACTAGTTAAAAAAACAATAGAAAAACTTAATAAATAA", "aa": "MDGRDTTFKIMPYAELKIFLTATPEERAMRRLNQNIALGFNMDYNKVLEEVKLRDYQDMNREVDPLHKVKEAKEIDCTNMGFEAVVVEIIELVKKTIEKLNK", "aa_hexdigest": "d55537de57d95860022538db926cbe00", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "cmk" ], "truncated": "3-prime", "pseudogene": { "start": 216127, "stop": 216735, "observations": { "mutation": [ 216651 ], "stop": [ 216651 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEA4", "query_cov": 1.0, "subject_cov": 0.4473684210526316, "identity": 1.0, "score": 202.0, "evalue": 3.19e-64, "gene_start": 1, "gene_end": 102, "reference_start": 127, "reference_end": 228, "reference_sequence": "MTKITKINIAIDGPCGAGKSTVSQEVARRLGYTFINSGSVYRALALNAKLLGVDWNNEEEINKTLEKDIITLDERENIFLREKNVSVEIREDSISYGSSTIATYKKVRQYVVNLIQNITKKGKGYIMDGRDTTFKIMPYAELKIFLTATPEERAMRRLNQNIALGFNMDYNKVLEEVKLRDYQDMNREVDPLHKVKEAKEIDCTNMGFEAVVVEIIELVKKTIEKLNK" }, "paralog": false, "identity": 0.995049504950495, "score": 391.0, "evalue": 3.82e-141, "description": "Internal stop codon at 216651. Nonsense mutation around 216651.", "psc": { "uniref90_id": "UniRef90_C4XEA4", "gene": "cmk", "product": "(d)CMP kinase", "ec_ids": [ "2.7.4.25" ], "uniref50_id": "UniRef50_A5IZG6", "go_ids": [ "GO:0004127", "GO:0005524", "GO:0015949" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEA4", "EC:2.7.4.25", "UniRef:UniRef50_A5IZG6", "GO:0004127", "GO:0005524", "GO:0015949" ] }, "pscc": { "uniref50_id": "UniRef50_A5IZG6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IZG6" ], "product": "Cytidylate kinase" } }, "id": "OAIKOHHJFB_423", "locus": "GCA000209735_00393" }, { "type": "cds", "sequence": "contig_1", "start": 216649, "stop": 216813, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13207.12", "PFAM:PF02224.24" ], "nt": "ATGACAAAAATTACAAAGATTAATATTGCAATTGATGGACCTTGCGGTGCAGGTAAATCAACTGTTTCACAAGAAGTAGCAAGAAGGCTTGGATACACATTTATTAATAGTGGAAGTGTGTATAGAGCATTAGCTTTAAATGCAAAATTATTAGGTGTTGATTGA", "aa": "MTKITKINIAIDGPCGAGKSTVSQEVARRLGYTFINSGSVYRALALNAKLLGVD", "aa_hexdigest": "2701fd7a256c8bd6ee28a59ae86263a8", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13207.12", "name": "AAA_17", "length": 38, "aa_cov": 0.7037037037037037, "hmm_cov": 1.0, "evalue": 8.083867100475973e-09, "score": 31.983428955078125, "start": 13, "stop": 50 }, { "id": "PF02224.24", "name": "Cytidylate_kin", "length": 40, "aa_cov": 0.7407407407407407, "hmm_cov": 1.0, "evalue": 2.002576479185176e-16, "score": 56.42131042480469, "start": 9, "stop": 48 } ], "seq_stats": { "molecular_weight": 5683.5617, "isoelectric_point": 9.743251991271972 }, "id": "OAIKOHHJFB_424", "locus": "GCA000209735_00394" }, { "type": "cds", "sequence": "contig_1", "start": 216902, "stop": 217132, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGTTTTTAACTCAAATTATAAACTTCAAAGTGTTGATTTATATACCTTTGTAGATAGATTAGAAAAATTAATCAAAAATTCCCAATTGCCCTATGAAAAAGACAAAAAAGGAACTGAAAAAGCTGAAGAAAAATATAAAAATTTTAAAAGAAATTTTCTTGAACCTATTGACAATATGAAGCAAATTATAAATGATACAGTGCTATTAATTAATGAATACAAATAA", "aa": "MIVFNSNYKLQSVDLYTFVDRLEKLIKNSQLPYEKDKKGTEKAEEKYKNFKRNFLEPIDNMKQIINDTVLLINEYK", "aa_hexdigest": "6ac33935e39f8d85c25834f75ffd5f4b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9179.484499999995, "isoelectric_point": 8.802593803405763 }, "id": "OAIKOHHJFB_425", "locus": "GCA000209735_00395" }, { "type": "cds", "sequence": "contig_1", "start": 217541, "stop": 217924, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGAATATGAAAAAAATAATTTTGTTAGCAATCTAAAACAAAATGTCTCTATTTATACAAATAACTTTAATAAATTAGTTAAATTTATGGACAAGGATAACCACAAATTGTCATATCAATCATTTTATTATAAGGAATATGAAAAAACTAAAGAAATAACTGATTTCACATTTGAAAACTTTTTATTAAAAAACTCAAAAGCTTTTGAAGAAAAAGCATATAAATTTATGGTTAATAATGTTGATGAAAGTACTAAATTCAAAAAATATTTTGAAGACTTTACATCTACTCCTAGTGATAATACTAATTTTAATTTTTATAAAAATGTAACCTATTTGAAGAAAATAAATGATGAAATAAATTCAATTTTAAATGACTGA", "aa": "MGEYEKNNFVSNLKQNVSIYTNNFNKLVKFMDKDNHKLSYQSFYYKEYEKTKEITDFTFENFLLKNSKAFEEKAYKFMVNNVDESTKFKKYFEDFTSTPSDNTNFNFYKNVTYLKKINDEINSILND", "aa_hexdigest": "d57bb2f903f709e1ed7fd177013c66c6", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15383.972799999987, "isoelectric_point": 6.605282020568848 }, "id": "OAIKOHHJFB_426", "locus": "GCA000209735_00396" }, { "type": "cds", "sequence": "contig_1", "start": 218002, "stop": 218385, "strand": "-", "frame": 2, "gene": null, "product": "Pseudouridine synthase", "db_xrefs": [ "EC:5.4.99.22", "KEGG:K06178", "SO:0001217", "UniRef:UniRef50_A0A454AP59", "UniRef:UniRef90_C4XEA6" ], "nt": "ATGATTAATAAACTAACACATCCTAAATACGAAATTTTACGTACATATCGTGTTAGAATTGATTCTCCTTTAAGCTTAAAAGAATTCAAATTAATTAACGCTGGTTTGATTGTTAATGGTAAGTTTTCAAAACAAATAGTTGACCAAGTTGATACTAAAAGTTATTTAGTAACTCTTCATGTCGGCTCATACCATCACATCAAAGAATTATTCAAAGCATTAAATCATGAAGTTATTAATCTTAAAAGAGTGTCATTTGCTAACTTAACAGTTGAAAAAATGCCTGAAGGCTCATATCGAACTTTAACTCTTAAAGAAATAAAAGATCTTAAAGTTTTAATTTCATTGCAAGAAAAGAAATTATGTCAAAAAGAAGAAAATTAA", "aa": "MINKLTHPKYEILRTYRVRIDSPLSLKEFKLINAGLIVNGKFSKQIVDQVDTKSYLVTLHVGSYHHIKELFKALNHEVINLKRVSFANLTVEKMPEGSYRTLTLKEIKDLKVLISLQEKKLCQKEEN", "aa_hexdigest": "55c819be69260b4bad3d8a552b4cfb79", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 218002, "stop": 218685, "observations": { "mutation": [ 218391 ], "stop": [ 218391 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEA6", "query_cov": 1.0, "subject_cov": 0.4721189591078067, "identity": 1.0, "score": 248.0, "evalue": 3.89e-81, "gene_start": 1, "gene_end": 127, "reference_start": 143, "reference_end": 269, "reference_sequence": "MYTRTTKNLWQMNEINFLVERFAMKELIRVQKLIAQTGYCSRRKAEEYIEKKLVKVNGKIVQLGDKASENDEIKINNQLISLQKPNFVYFLLNKPKKTISTVKDPKGRKTVVDLINYSERIAPVGRLDYDTTGALLLTNDWEMINKLTHPKYEILRTYRVRIDSPLSLKEFKLINAGLIVNGKFSKQIVDQVDTKSYLVTLHVGSYHHIKELFKALNHEVINLKRVSFANLTVEKMPEGSYRTLTLKEIKDLKVLISLQEKKLCQKEEN" }, "paralog": false, "identity": 0.9955947136563876, "score": 441.0, "evalue": 1.01e-159, "description": "Internal stop codon at 218391. Nonsense mutation around 218391.", "psc": { "uniref90_id": "UniRef90_C4XEA6", "product": "Pseudouridine synthase", "ec_ids": [ "5.4.99.22" ], "uniref50_id": "UniRef50_A0A454AP59", "kegg_orthology_id": "K06178", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEA6", "EC:5.4.99.22", "UniRef:UniRef50_A0A454AP59", "KEGG:K06178" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454AP59", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454AP59" ], "product": "Pseudouridine synthase" } }, "id": "OAIKOHHJFB_427", "locus": "GCA000209735_00397" }, { "type": "cds", "sequence": "contig_1", "start": 218389, "stop": 218778, "strand": "-", "frame": 2, "gene": null, "product": "pseudouridine synthase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FC9400C" ], "nt": "ATGAATGAAATAAATTTTTTGGTAGAAAGGTTTGCAATGAAAGAATTGATTAGGGTTCAAAAACTAATCGCACAAACAGGATATTGTTCAAGAAGAAAAGCTGAAGAATATATTGAAAAAAAACTAGTTAAAGTTAATGGAAAAATTGTTCAATTAGGTGACAAAGCAAGTGAAAACGATGAGATAAAAATTAACAATCAACTAATCTCATTACAAAAACCAAATTTTGTATATTTCCTATTAAATAAGCCTAAAAAGACAATTTCAACAGTTAAAGATCCTAAGGGCAGAAAAACAGTTGTTGATTTAATTAATTACTCAGAAAGAATTGCTCCTGTAGGTCGTTTAGACTATGATACAACTGGCGCTTTGTTATTAACAAATGATTGA", "aa": "MNEINFLVERFAMKELIRVQKLIAQTGYCSRRKAEEYIEKKLVKVNGKIVQLGDKASENDEIKINNQLISLQKPNFVYFLLNKPKKTISTVKDPKGRKTVVDLINYSERIAPVGRLDYDTTGALLLTND", "aa_hexdigest": "67f54b5998efcf146e26742f1cbe475a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FC9400C", "query_cov": 0.9147286821705426, "subject_cov": 0.9147286821705426, "identity": 0.508, "score": 112.0, "evalue": 1.1e-29, "valid": false, "product": "pseudouridine synthase", "uniref50_id": "UniRef50_UPI002FC9400C" }, "pscc": { "uniref50_id": "UniRef50_UPI002FC9400C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FC9400C" ], "product": "pseudouridine synthase" }, "genes": [], "id": "OAIKOHHJFB_428", "locus": "GCA000209735_00398" }, { "type": "cds", "sequence": "contig_1", "start": 219051, "stop": 219356, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08668.18" ], "nt": "ATGGTACCTGAAAAAGTTAACCAATATATAGGAAGTCACTTTTTATATGCTTTTGAAATAGTTCACGCTTTGCTTAGTGCAAAAAGAGCAAAACATTCAGTTTTTGCAGCAACTTTTGCTGCTGAGTTAGCTAAAACAATAAAATATGATGCAAAAGTGGCTTACTATGCTGGACTATTTCATGATATTTGTAAAGAATTAAGTGAAAATGAAAGTAGAAAATTTATTGCTCAATATGATAAAAAAGCTAACAACAAATCTATTTATCCAAATCATGTTTTACATCAAGTTTGTGGAGCTTTATGA", "aa": "MVPEKVNQYIGSHFLYAFEIVHALLSAKRAKHSVFAATFAAELAKTIKYDAKVAYYAGLFHDICKELSENESRKFIAQYDKKANNKSIYPNHVLHQVCGAL", "aa_hexdigest": "d91cccf887183e7bb44afaeda972a71e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08668.18", "name": "HDOD", "length": 62, "aa_cov": 0.6039603960396039, "hmm_cov": 1.0, "evalue": 1.1822153396779323e-06, "score": 24.255308151245117, "start": 29, "stop": 89 } ], "seq_stats": { "molecular_weight": 11435.049399999989, "isoelectric_point": 9.077486991882324 }, "id": "OAIKOHHJFB_429", "locus": "GCA000209735_00399" }, { "type": "cds", "sequence": "contig_1", "start": 219513, "stop": 219860, "strand": "-", "frame": 3, "gene": null, "product": "nicotinate-nucleotide adenylyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBE0" ], "nt": "ATGAAAATAGGATTATTTGGTGGATCATTCAATCCAATCCATAGTGGACATATCAAAATTGCTGAATATGCCTATAAAACACTTAAATTAGACAAAATGTTTTTTATACCAACAGCGATAAGCCCATTTAAAAAGAATAATAAAGTAGCCCCTAATAAAGACAGAGTTAATATGATTAATATAGCTATTGAAAATCTAGAAGGCAATTATGCTGTGCATGATTTTGAAATCAAAAAAGGTGGCGTTAGTTATACTTTTGAAACAATTAGATATTTCAAACAACAATATCCAAATGATGAATTATATTTCTTAATAGGCAGCGATTTATTGCCAAAATTAAATAAATGA", "aa": "MKIGLFGGSFNPIHSGHIKIAEYAYKTLKLDKMFFIPTAISPFKKNNKVAPNKDRVNMINIAIENLEGNYAVHDFEIKKGGVSYTFETIRYFKQQYPNDELYFLIGSDLLPKLNK", "aa_hexdigest": "b1dec7b34b8b3b87832ee5bfd24113d9", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A432FBE0", "query_cov": 1.0, "subject_cov": 1.0267857142857142, "identity": 0.5479999999999999, "score": 123.0, "evalue": 3.13e-34, "valid": false, "gene": "nadD", "product": "nicotinate (nicotinamide) nucleotide adenylyltransferase", "ec_ids": [ "2.7.7.18" ], "uniref50_id": "UniRef50_A0A432FBE0", "go_ids": [ "GO:0000309", "GO:0004515", "GO:0005737", "GO:0009435" ] }, "pscc": { "uniref50_id": "UniRef50_A0A432FBE0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBE0" ], "product": "nicotinate-nucleotide adenylyltransferase" }, "genes": [], "id": "OAIKOHHJFB_430", "locus": "GCA000209735_00400" }, { "type": "cds", "sequence": "contig_1", "start": 220507, "stop": 220833, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF11798.14" ], "nt": "GTGTTTTCAATTGATGAATGTTGGCTTGATGTAACTGATATTATCAAAAATAGAGATCCAAAAGATTTGGCTAAAAGTATCCAAAGTGACATATTAAATACTTTTGACATTCCTGCAACAATTGGAATTTCATACAACAAATTTTTAGCAAAATTATCAACTAATTTAGGTAAACCATTTGGAATTCTAGAAACAAAAGAGGAAGACATAGAAAAAAGGATTTGGCCTTTACCAATTATTGAATTTTTCGGTATTGGTGCTCCAACAGCTGAGAGATTAATTAGAATGCATATTGATACTATTGGCAAATTAGCAAAATGTAATTGA", "aa": "MFSIDECWLDVTDIIKNRDPKDLAKSIQSDILNTFDIPATIGISYNKFLAKLSTNLGKPFGILETKEEDIEKRIWPLPIIEFFGIGAPTAERLIRMHIDTIGKLAKCN", "aa_hexdigest": "6a332b7d0156303f109f8fd90ed536aa", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF11798.14", "name": "IMS_HHH", "length": 31, "aa_cov": 0.28703703703703703, "hmm_cov": 1.0, "evalue": 7.316905127758249e-07, "score": 25.29746437072754, "start": 68, "stop": 98 } ], "seq_stats": { "molecular_weight": 12236.127199999983, "isoelectric_point": 5.35516529083252 }, "id": "OAIKOHHJFB_431", "locus": "GCA000209735_00401" }, { "type": "cds", "sequence": "contig_1", "start": 220960, "stop": 221136, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAATAAAAAAACAATTTTTCACATGGATTTTGATTCATATTTTGTAAGTGCTCATCGCTCAATTAATCCTGAATTAAAAGGAAAATCAGTAGCTATTGGTAGAAGATTAAAAAGATCAATAGTTTCTTCAATTAGTTATGAGTTAAAAAGAAAAGGCGCTAAAGTTGGCTGA", "aa": "MENKKTIFHMDFDSYFVSAHRSINPELKGKSVAIGRRLKRSIVSSISYELKRKGAKVG", "aa_hexdigest": "607e38b44feedb1fde82dc651c692c95", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6612.6438, "isoelectric_point": 10.345902442932129 }, "id": "OAIKOHHJFB_432", "locus": "GCA000209735_00402" }, { "type": "cds", "sequence": "contig_1", "start": 221126, "stop": 221554, "strand": "-", "frame": 1, "gene": "sufU", "product": "Fe-S cluster assembly sulfur transfer protein SufU", "db_xrefs": [ "GO:0005198", "GO:0016226", "RefSeq:WP_013526700.1", "SO:0001217", "UniParc:UPI0001F59E8A", "UniRef:UniRef100_A0AB32XAS2", "UniRef:UniRef50_C4XEA9", "UniRef:UniRef90_C4XEA9" ], "nt": "TTGTCTTATAATAAAGATCAAAAACGTCAAATAATAATGGAACATTATTTGAAACCTAGACACAAAAGTTCAAGTTTAAAAAACAAAATTATTAAGCACGGAGAAAGCTGTGCTGATTATTTAGAATGTGAACTAAATATAAAAAATAATCAAATTGAAGACATTAAATTTGATGGTCAAGGTTGTGCATTTTTTATAGCTTCAACTGATTTATTAATCGACTTATTAAAAGGTAAAAAAATTGACGAAAGCATCTGTTTGATAGAACTTTACGAAAAATTCATACTAGATGAAAAATTAAATGAAAATGATATTGAAAAGCTAGAAACTTTAGCTATTTTTGATAATGTTAAAACACAATTTAATCGCGTTAATTGTGCTTTAATGTTAGCTAGAGCAATAAAGGAAAAATTGCAAGATGGAAAATAA", "aa": "MSYNKDQKRQIIMEHYLKPRHKSSSLKNKIIKHGESCADYLECELNIKNNQIEDIKFDGQGCAFFIASTDLLIDLLKGKKIDESICLIELYEKFILDEKLNENDIEKLETLAIFDNVKTQFNRVNCALMLARAIKEKLQDGK", "aa_hexdigest": "2e35a2eeba09b0c09c824cfe5cee97ee", "start_type": "TTG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001F59E8A", "ncbi_nrp_id": "WP_013526700.1", "uniref100_id": "UniRef100_A0AB32XAS2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59E8A", "RefSeq:WP_013526700.1", "UniRef:UniRef100_A0AB32XAS2" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XAS2", "uniref90_id": "UniRef90_C4XEA9" }, "psc": { "uniref90_id": "UniRef90_C4XEA9", "gene": "sufU", "product": "Fe-S cluster assembly sulfur transfer protein SufU", "uniref50_id": "UniRef50_C4XEA9", "go_ids": [ "GO:0005198", "GO:0016226" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEA9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEA9" ], "product": "NIF system FeS cluster assembly NifU N-terminal domain-containing protein" }, "genes": [ "sufU" ], "id": "OAIKOHHJFB_433", "locus": "GCA000209735_00403" }, { "type": "cds", "sequence": "contig_1", "start": 221544, "stop": 221966, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00266.25" ], "nt": "TTGTCAACAGCAGCTGATCGCTTTGAAGCTGGAACACCTAATTTAGCAGCAATTAATATGTTTAGTGTTTCAATAAACTTTTTTAATCAAATTGGTTATTGTGAAACTCAAAAAATTTTAGACGAATTAAGCTTTTATGCTTATCAAGAATTAAGCAAAATTGAAGGCATAAAACTATATAGCAATCCTAAAAATCACATTATAATATTCAATATAAAAAACTATAATAGTCATGATGTTTCACATTATTTAGGAACAAAAAATATTTATGTAAGAGCAGGTGTATTTTGTGATCATTATCTTAAAAAAATCACCAAAGAAAAGTCTTTTGTAAGAGTTTCTTTGGGTATTTATAACACCAAAAAAGATATTGATAAATTAGTTAAAGCATTAAAAGAAGGAGGTGATTTCCTTGTCTTATAA", "aa": "MSTAADRFEAGTPNLAAINMFSVSINFFNQIGYCETQKILDELSFYAYQELSKIEGIKLYSNPKNHIIIFNIKNYNSHDVSHYLGTKNIYVRAGVFCDHYLKKITKEKSFVRVSLGIYNTKKDIDKLVKALKEGGDFLVL", "aa_hexdigest": "74f1b85d2cf308b30f7136d608f54491", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00266.25", "name": "Aminotran_5", "length": 128, "aa_cov": 0.8857142857142857, "hmm_cov": 1.0, "evalue": 9.474684898452274e-30, "score": 99.8290023803711, "start": 4, "stop": 127 } ], "seq_stats": { "molecular_weight": 16000.246899999984, "isoelectric_point": 8.969631385803225 }, "id": "OAIKOHHJFB_434", "locus": "GCA000209735_00404" }, { "type": "cds", "sequence": "contig_1", "start": 222339, "stop": 222704, "strand": "-", "frame": 3, "gene": null, "product": "aminotransferase class V-fold PLP-dependent enzyme", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C98C43" ], "nt": "ATGAAAACATTAGACAAATTAATTCAAGAACAATTCCCTTTAGCAAAAAAGATAACTTATTTGGATAGTAGTGCATTAGTTTTAAAACCAAAAATGGCTATTGATGCAGTTAACAATTTTTATTTAAATGAGTCAATTAGTACCCGTACTATTAACACTAAATTAGGAATTGAAAGCGCGCAAAAAATAAACAACTTACGTAAAAAAGTTGCTACTTTGTTGTCTTGTCAAGAACAAGAAGTTATATTTACTTCAGGAGCAACTGATTCACTAAACAAGTTTGCTTTAATGATCAAAAATCAAATTAAAAAAGGGGATATTATCCTTTTAGATGTTTATAATCATTCTTCAAATATGGCGCCTTGA", "aa": "MKTLDKLIQEQFPLAKKITYLDSSALVLKPKMAIDAVNNFYLNESISTRTINTKLGIESAQKINNLRKKVATLLSCQEQEVIFTSGATDSLNKFALMIKNQIKKGDIILLDVYNHSSNMAP", "aa_hexdigest": "2f416e543c03322904c436cf14dba10b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028BE3002", "query_cov": 0.9421487603305785, "subject_cov": 0.8837209302325582, "identity": 0.526, "score": 117.0, "evalue": 1.75e-31, "valid": false, "product": "aminotransferase class V-fold PLP-dependent enzyme", "uniref50_id": "UniRef50_UPI0024C98C43" }, "pscc": { "uniref50_id": "UniRef50_UPI0024C98C43", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C98C43" ], "product": "aminotransferase class V-fold PLP-dependent enzyme" }, "genes": [], "id": "OAIKOHHJFB_435", "locus": "GCA000209735_00405" }, { "type": "cds", "sequence": "contig_1", "start": 222935, "stop": 223390, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13692.12" ], "nt": "TTGGTTTGGGAAAAAAATATTAAAAAAGTTCTATTAACTTATAAAAAACTTGTTGAAGAAAATTCTAATTACCATTTAACAATGGTTGGTGGTGATATCAATTACAAGGAAATTGTTAAATATGCCTATAAAATAGGTTTAAAAGATAAAATATTGTTTACTGGCCCTATAGAAAATCAAGAAGAATTAAAAGGCATTTATTTATCTCATGATCTTTTTTTCTATCCAAGTTTATATGAAACTTATGGACTTGTTGTTAGAGAATCTGCAGCTTTAAAGTTGCCTCTCTTAGTTGTTGAAAACACAGCATGTGCCGAAAATGTGATAGATGGACAAAATAGTTTTATTTGCCAAGACGATGTTGACAATATGTACGAAAAAATTAAACAAATTTTTAAAGATAAAAATAATCTAATTAATGTGAAAAATAATATGAACAATTTATTTAATTCATGA", "aa": "MVWEKNIKKVLLTYKKLVEENSNYHLTMVGGDINYKEIVKYAYKIGLKDKILFTGPIENQEELKGIYLSHDLFFYPSLYETYGLVVRESAALKLPLLVVENTACAENVIDGQNSFICQDDVDNMYEKIKQIFKDKNNLINVKNNMNNLFNS", "aa_hexdigest": "0b68726ac7b29655a1b4aa37cd2c1a18", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13692.12", "name": "Glyco_trans_1_4", "length": 135, "aa_cov": 0.8675496688741722, "hmm_cov": 0.9555555555555556, "evalue": 1.205471613906785e-19, "score": 67.07129669189453, "start": 4, "stop": 134 } ], "seq_stats": { "molecular_weight": 17527.011300000006, "isoelectric_point": 5.728370094299317 }, "id": "OAIKOHHJFB_436", "locus": "GCA000209735_00406" }, { "type": "cds", "sequence": "contig_1", "start": 223433, "stop": 223564, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCAAAAGTTGCGCAAAAAGCTTTAATTGAATATGGATATAAAAAAGAAAGTGAAATTGTACAATTAGGGACAAATTTCTTTTATCCAGAAAATGCAGATGAATTAAAAAATAAAGTTATAAAAAAATGA", "aa": "MSKVAQKALIEYGYKKESEIVQLGTNFFYPENADELKNKVIKK", "aa_hexdigest": "e5d117b08f961fae864ee84be65e4df8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4993.729100000001, "isoelectric_point": 9.004702186584474 }, "id": "OAIKOHHJFB_437", "locus": "GCA000209735_00407" }, { "type": "cds", "sequence": "contig_1", "start": 223790, "stop": 224050, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAATAGTGAAATTAGAGTTATTTTAATTAATGATAGTTTTACTCCTGTAATAGATGGAGTTGTCAGGGTAATGCAAAATTATGCTTCTAATTTTATTAAAAAAAATGTTAAAGTTCTGGTTTTGGCTCCAGAATACAAAAAAATGAAATTAGATGAAGATAAAGGTCTTAATTATTCTGTCATTAGAATACCTAGACAATTAGCTAAAATTGGTGGTTATGAAGTAATGAACACTATTTTGAGTAGAAAAATTTGA", "aa": "MTNSEIRVILINDSFTPVIDGVVRVMQNYASNFIKKNVKVLVLAPEYKKMKLDEDKGLNYSVIRIPRQLAKIGGYEVMNTILSRKI", "aa_hexdigest": "91b8d0591ea5591d0999f147bee4d1b0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9842.572999999995, "isoelectric_point": 9.791796684265137 }, "id": "OAIKOHHJFB_438", "locus": "GCA000209735_00408" }, { "type": "cds", "sequence": "contig_1", "start": 224136, "stop": 224357, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAAAAACCAAATTATCAAAAAGCTCTTGAGACTTTGTATGAACCAAATTCAAATATTCATCATGTAATTAATTGGCCTTTTAAAAAGGCTACAAAAATTAACATATATGAAAATATCAATTTTTTTGATACTATTGATGTTAAATTTGAAGATATAAAAATTCGGGTGCCAAAAAATTATGAAACAATATTTTTACAAAACTATGGTCCAAATTGA", "aa": "MFKKPNYQKALETLYEPNSNIHHVINWPFKKATKINIYENINFFDTIDVKFEDIKIRVPKNYETIFLQNYGPN", "aa_hexdigest": "0595a260e0fd2fc1e42b5d7c9cb9dc8d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8838.021799999995, "isoelectric_point": 8.89278507232666 }, "id": "OAIKOHHJFB_439", "locus": "GCA000209735_00409" }, { "type": "cds", "sequence": "contig_1", "start": 224397, "stop": 224567, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCCCTAAATTTTCAAAAGATAAAGATTCTCAAAAAGATGTAACTTTTTTAGATCTTTTTATCGATATACCAACAACTAAAAAAAGAATAAAAAAATATTTATCTCTTAAAAATAAATTGGGTTATTTGAACGCTTATTCTCAAAGAAAGTTTTTTGATATTCAATGA", "aa": "MFPKFSKDKDSQKDVTFLDLFIDIPTTKKRIKKYLSLKNKLGYLNAYSQRKFFDIQ", "aa_hexdigest": "4bef104fc0092c4b94310fbf3e1ec783", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6751.8451000000005, "isoelectric_point": 9.8733491897583 }, "id": "OAIKOHHJFB_440", "locus": "GCA000209735_00410" }, { "type": "cds", "sequence": "contig_1", "start": 224670, "stop": 224804, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04991.19" ], "nt": "ATGAATGAAAAACAAACTAAAGTTTATGAACTGTTGAAAGAGTTTATTGAAATTTGTAATAAACACAATTTAGATTATTGTGCTATGTATGGAACAGCATTAGGAGCAATAAGACACAATGGATTTATACCTTGA", "aa": "MNEKQTKVYELLKEFIEICNKHNLDYCAMYGTALGAIRHNGFIP", "aa_hexdigest": "d5025df6f2ee5b016ad3728226234bea", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04991.19", "name": "LicD", "length": 26, "aa_cov": 0.5909090909090909, "hmm_cov": 1.0, "evalue": 3.105400183289342e-09, "score": 33.315032958984375, "start": 19, "stop": 44 } ], "seq_stats": { "molecular_weight": 5117.8992, "isoelectric_point": 6.6983842849731445 }, "id": "OAIKOHHJFB_441", "locus": "GCA000209735_00411" }, { "type": "cds", "sequence": "contig_1", "start": 224881, "stop": 225870, "strand": "-", "frame": 2, "gene": null, "product": "D-lactate dehydrogenase", "db_xrefs": [ "EC:1.1.1.399", "KEGG:K25634", "RefSeq:WP_041594192.1", "SO:0001217", "UniParc:UPI0002FBDD0C", "UniRef:UniRef100_C4XEB3", "UniRef:UniRef50_A0A3P8K9L4", "UniRef:UniRef90_C4XEB3" ], "nt": "ATGAAAATTATTACTTTTAAAGTTAGAAATGTTGAAAAACCAATATTTGAAGAAATTAACAAAAAATTTGGTTATAAACTTGAAATTCATAGTGAAAGTTTAACTATGGAAAATGTCAATTTAACAAAAGGTTTTGACGCTGTTATTGTTAGAGTTGATGGTTCAATAAATAAAGAAATGTTAGATGCATTAAAAGCAAATGGCATTAAATATCTTTTAACAAGAACTGTCAGAACAGACCATATTGATTTAGAATATGCTCATAAATTAGGCTTTAAAATGGCTAGAGTTCCTTCATATTCACCAACAGCAATTGCTGAATTAGCTTTTGCTCATGCTTTAATGTTATCAAGACAAACACTTCATTTTGGACATAAAGGTTTTAACAAAGACTTTACTATTGATCCTGAAGGTTTTGCTACTGAATTAAAAAATAAAACAGTTGGAATAGTTGGGGTTGGAAAAATAGGACTTGCAGCTGCTAAAATGTTTAAAGTTATGACAAACAATGTTTTAGGCTTCGATTTATATCCTTCAGAAGAAGGCAAAAAAGTTGTTAAATATACAAAAACTCTAGAAGAATTAGTTAAAAAATCAGATATCATTTCTTTCCATTGCCCCTATATTAAAGGTAAAAACGACAAAATGATTAATGATGCATTAATTAAGCAAATGAAAAAAGGTGTAATTATTGTTAACACAGCTAGAGGCCAAATTCAAGATGAAGAAGCATTATTAAAAGGTCTAAAAAGTGGCAAAATTCAAGCTATTGCTACAGATGTTTTGAACAATGAAAAATCAATTTTCTTTAAAAAGCATGCAGAAATAGAAAACAAAACATTTCGCCAATTATTAGAATTCTATCCTCGTTTTGTTTTAACACCTCACATTGGTTCTTATACTGATGAAGCTGTTGAAAACATGGTTGAATACACTTATGACAATCTAAAAGAATATATCGAAACTGATCAATGCAAAAATGCAATTTAA", "aa": "MKIITFKVRNVEKPIFEEINKKFGYKLEIHSESLTMENVNLTKGFDAVIVRVDGSINKEMLDALKANGIKYLLTRTVRTDHIDLEYAHKLGFKMARVPSYSPTAIAELAFAHALMLSRQTLHFGHKGFNKDFTIDPEGFATELKNKTVGIVGVGKIGLAAAKMFKVMTNNVLGFDLYPSEEGKKVVKYTKTLEELVKKSDIISFHCPYIKGKNDKMINDALIKQMKKGVIIVNTARGQIQDEEALLKGLKSGKIQAIATDVLNNEKSIFFKKHAEIENKTFRQLLEFYPRFVLTPHIGSYTDEAVENMVEYTYDNLKEYIETDQCKNAI", "aa_hexdigest": "1680e4c93dc0b833ce5a656ba1f61eac", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0002FBDD0C", "ncbi_nrp_id": "WP_041594192.1", "uniref100_id": "UniRef100_C4XEB3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002FBDD0C", "RefSeq:WP_041594192.1", "UniRef:UniRef100_C4XEB3" ] }, "ips": { "uniref100_id": "UniRef100_C4XEB3", "uniref90_id": "UniRef90_C4XEB3" }, "psc": { "uniref90_id": "UniRef90_C4XEB3", "product": "D-lactate dehydrogenase", "ec_ids": [ "1.1.1.399" ], "uniref50_id": "UniRef50_A0A3P8K9L4", "kegg_orthology_id": "K25634" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8K9L4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8K9L4" ], "product": "D-lactate dehydrogenase" }, "genes": [], "id": "OAIKOHHJFB_442", "locus": "GCA000209735_00412" }, { "type": "cds", "sequence": "contig_1", "start": 225909, "stop": 226043, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATTTTTGCAGCTACACCACCTGCTACAGTTGCTGTTGGTTTTGCAATTAGTGAAAATAAATGTGCTAGATTAGCAAGCTCTGCTTCAGCATTTAGTACATTACTTGCAGTTATCTTTTTACCTATTTGA", "aa": "MVIFAATPPATVAVGFAISENKCARLASSASAFSTLLAVIFLPI", "aa_hexdigest": "c24c58320307d048a84b3244beda3903", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4468.282899999997, "isoelectric_point": 7.975980186462403 }, "id": "OAIKOHHJFB_443", "locus": "GCA000209735_00413" }, { "type": "cds", "sequence": "contig_1", "start": 226290, "stop": 226490, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATCAATTAATACCAGGTGCAGGAGCAACATATAAAGATTTTAGTCAAACTATTAAAGTTAATGGAAGTGATGTAGTTATTAAATATTCAGCTACTTTTGGTGAAAACTTTAAAACAGTTTTAACACCAGACAAAAAAGTATTTTATATGTTAAATGCAAATAATGAATATATTAAAACCACTAAACCAGTTGGTTGA", "aa": "MNQLIPGAGATYKDFSQTIKVNGSDVVIKYSATFGENFKTVLTPDKKVFYMLNANNEYIKTTKPVG", "aa_hexdigest": "fa4802d779d6d150b0055bd852f23f15", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7308.300000000002, "isoelectric_point": 9.302352714538571 }, "id": "OAIKOHHJFB_444", "locus": "GCA000209735_00414" }, { "type": "cds", "sequence": "contig_1", "start": 226491, "stop": 226598, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAATGAAAGGTTTAAAATTCAACAAAGAAAATATTAAAGCTTCAGCAAAATCAATGTTTTTAAACCCAATTATTATTGCAACTTTTGCTGGATTAATTCTTTGA", "aa": "MQMKGLKFNKENIKASAKSMFLNPIIIATFAGLIL", "aa_hexdigest": "78cfbf956a516b43818a46747f703483", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3882.7434999999987, "isoelectric_point": 10.176350593566895 }, "id": "OAIKOHHJFB_445", "locus": "GCA000209735_00415" }, { "type": "cds", "sequence": "contig_1", "start": 226734, "stop": 226847, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTCATAAAACACCAGAATCAGTGGAAAATGCTCAAGAAAACACTATTATTGGTGAAATGAGTGAAACAAAAACAGTTGATGAATCTCGTCATCGCGCAATGGTTGTTTGA", "aa": "MPHKTPESVENAQENTIIGEMSETKTVDESRHRAMVV", "aa_hexdigest": "10755b970deafaacb484efd14149e65c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4182.627900000001, "isoelectric_point": 5.086316871643065 }, "id": "OAIKOHHJFB_446", "locus": "GCA000209735_00416" }, { "type": "cds", "sequence": "contig_1", "start": 226863, "stop": 226994, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTGCTTTACCAGCTTTAGCACTCAAAGGCTTTATGAGCGATATTAGTCTTGGACTTCTTCAAGAACAAGGTATTATCTTAGGAATTGCATTTGGATTCTATATCTTGCTTTCATTAGTGGCTTGA", "aa": "MKIALPALALKGFMSDISLGLLQEQGIILGIAFGFYILLSLVA", "aa_hexdigest": "8d80b6e757dbfe528ccce887fcdb80e1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4550.551999999999, "isoelectric_point": 5.8228364944458 }, "id": "OAIKOHHJFB_447", "locus": "GCA000209735_00417" }, { "type": "cds", "sequence": "contig_1", "start": 227550, "stop": 227801, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATGCTATTGATCCTTTTGTTTCTATATTCTTTAGATATGTTGTTTCATTGGTTGTTTTAGGTAGCATTGTTTTACCATCAGCAAAAATCTATGAAAAAATGGATGAACTATTTGCTAATACAAACATTTTATTAATTGTTGCTTCTGGAATTGTGGGTTGCATTTCATATATGACATTCTATTATGCACTTGATAAAATAGGTGTTCCAATGGCTACAGGATTAAATATTTCCTATGTTGGTTGA", "aa": "MKNAIDPFVSIFFRYVVSLVVLGSIVLPSAKIYEKMDELFANTNILLIVASGIVGCISYMTFYYALDKIGVPMATGLNISYVG", "aa_hexdigest": "3e0fff2e79b66575fee525df8745586a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9090.746299999993, "isoelectric_point": 5.996877479553221 }, "id": "OAIKOHHJFB_448", "locus": "GCA000209735_00418" }, { "type": "cds", "sequence": "contig_1", "start": 227868, "stop": 228086, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTATGACAATGTATGTCCTTGGTATTGTTTATGTCGGTACTGGAATAAGTAGTGCAATTACAGTTTGTTATCCTATTGTTGGCGCAATGCTTGCTTTTATCTTTTTGAAACAAAAAACTACAAAAAATGGTTTATTTGGATCAATAATTTCGCTTTTATGTATTTTAACTTTAGGAATTTTACAATTTCATAAAGGCGATATTAAAAATGGATGA", "aa": "MGMTMYVLGIVYVGTGISSAITVCYPIVGAMLAFIFLKQKTTKNGLFGSIISLLCILTLGILQFHKGDIKNG", "aa_hexdigest": "02f003578eb20d1715acdfe84f1f5df8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7686.295599999993, "isoelectric_point": 9.391705894470213 }, "id": "OAIKOHHJFB_449", "locus": "GCA000209735_00419" }, { "type": "cds", "sequence": "contig_1", "start": 228327, "stop": 228437, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGATAAAAATTCAAATGTAGAAGTTAATGAAATTCAAAACGATAAAAAAGTTAAAAATAAAAATTTATTTAATAAATTTGTTGCTTTATTTTCAGGGATTATGTGA", "aa": "MNDKNSNVEVNEIQNDKKVKNKNLFNKFVALFSGIM", "aa_hexdigest": "f5070d8a45990f166b5e2f6e68fd5c90", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4171.753500000001, "isoelectric_point": 9.303642082214353 }, "id": "OAIKOHHJFB_450", "locus": "GCA000209735_00420" }, { "type": "cds", "sequence": "contig_1", "start": 228566, "stop": 228775, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATAATCTATTTTTTTCTTCTTTAGTTGGTTTTCTTTGTAAATATGTTTCTAAAAGTCAATTAGCTTCTTTGACACCATATTCATTATCCATGCACATACCAGCTAAATCTAAAAATGGATCACTATTAGCCGCATATTCTCAATCAATTAATTTAATTTTATTATCCTTATTTATTAAGATATTTCCTGAAGTTGAATCAATATGA", "aa": "MYNLFFSSLVGFLCKYVSKSQLASLTPYSLSMHIPAKSKNGSLLAAYSQSINLILLSLFIKIFPEVESI", "aa_hexdigest": "1399650964bfb56fc1c382f6599735c2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7664.005399999998, "isoelectric_point": 9.25226078033447 }, "id": "OAIKOHHJFB_451", "locus": "GCA000209735_00421" }, { "type": "cds", "sequence": "contig_1", "start": 228709, "stop": 228984, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATTAGCTTTAAATAGTTATCGCAAACTACATAATCAAAATTTATCAGTTGATTTTGAATTTGATATTCTAAAAATTTTGGACTATTACATTTCAATAATTGGAGAAAAATACAAAAGATTTCCAAAACATTTATTAGACAATGAAGCTAAAATTAGAAAAATGCTTGCTTATCATAAAAGTAAAAATTATCCAAAAGTTTTATGTCATATTGATTCAACTTCAGGAAATATCTTAATAAATAAGGATAATAAAATTAAATTAATTGATTGA", "aa": "MELALNSYRKLHNQNLSVDFEFDILKILDYYISIIGEKYKRFPKHLLDNEAKIRKMLAYHKSKNYPKVLCHIDSTSGNILINKDNKIKLID", "aa_hexdigest": "d9c6b851be682b510d6594e446a48221", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10796.503999999984, "isoelectric_point": 9.32594814300537 }, "id": "OAIKOHHJFB_452", "locus": "GCA000209735_00422" }, { "type": "cds", "sequence": "contig_1", "start": 229027, "stop": 229434, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATATCTTAAAAGATATAGAGCTTAAGAAATACAATTGTGACCAAAAGGTCTATGAAATAGACGATTTCAAAGATTTATTGACATTTAATAGAAAAGCTCCTTGCTTTATAAACGATGTTTATTTAAACAAAATTAAAGAAGTTTTTAATGCCAAATTTGAAGATATTTTAGATATTCAACCAGTTAAAAAAGGAATGACTAATAACTCATTTTCTTTTAAAATAAAAGATAAAAAATATATTGCTAGAATACCAAAAGATGGCTCAAAGAGTTTAATTAATAGATACCAAGAAGAAAAAATTTATCAAAAACTAAAAGGCAATGAATTAGTTGAAAATTTAATTTATTTTGAAACCAAAAATAATATTGATGATCCTTTACATGGTTTTAAAATAACTGAATGA", "aa": "MDILKDIELKKYNCDQKVYEIDDFKDLLTFNRKAPCFINDVYLNKIKEVFNAKFEDILDIQPVKKGMTNNSFSFKIKDKKYIARIPKDGSKSLINRYQEEKIYQKLKGNELVENLIYFETKNNIDDPLHGFKITE", "aa_hexdigest": "5b961e929c2f8c8d42b547d59ec90c57", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16068.357999999991, "isoelectric_point": 8.37097797393799 }, "id": "OAIKOHHJFB_453", "locus": "GCA000209735_00423" }, { "type": "cds", "sequence": "contig_1", "start": 229858, "stop": 230046, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACAAAAATACTCAAATAATAATTTTAGCTGCTGGCTTTGGTTCAAGACTAACGCCTCTTTCACTTAAGACGCCAAAAGGATTATTAGAAATTAAAAAAGATTTAAAAATTGTTCCTCATCTTTTATCTTATTTAAAAGATTATAAAAATAAGACAATAGTAACTGGCTACAATTATCAATCTTGA", "aa": "MDKNTQIIILAAGFGSRLTPLSLKTPKGLLEIKKDLKIVPHLLSYLKDYKNKTIVTGYNYQS", "aa_hexdigest": "e210dcd2e2b6ed5bc32040c1362c3f38", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6993.214900000004, "isoelectric_point": 9.795277976989745 }, "id": "OAIKOHHJFB_454", "locus": "GCA000209735_00424" }, { "type": "cds", "sequence": "contig_1", "start": 230390, "stop": 230674, "strand": "+", "frame": 2, "gene": null, "product": "50S ribosomal protein L13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P644" ], "nt": "ATGGGTGATTATGTAATTGTTGTTAATGCCGAAAAAGTTATTTTAACAGCTAAAAAAGAAGAAGATAAAGTTTACTACCACCACTCAGGATATCCTGGTGGACTAAAAAGTATTACAGCTGCGAAATTAAGAGCTAAAAAACCTACAGCTCTTGTTGAAAAAGCAATCTATGGTATGTTACCACACACAAAATTAGGAAACAAACAACGTAAAAACTTAAGTGTTGTTGCAGGTCCTGACCACAAACACCAAGCACAAAAACCAGAAAGTTTAGAGGTAAGATAA", "aa": "MGDYVIVVNAEKVILTAKKEEDKVYYHHSGYPGGLKSITAAKLRAKKPTALVEKAIYGMLPHTKLGNKQRKNLSVVAGPDHKHQAQKPESLEVR", "aa_hexdigest": "029cc29247d3ecb5cd02735fdeba0c5b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P644", "query_cov": 1.0, "subject_cov": 0.831858407079646, "identity": 0.755, "score": 145.0, "evalue": 2.95e-43, "valid": false, "gene": "rplM", "product": "50S ribosomal protein L13", "uniref50_id": "UniRef50_A0A3B0P644", "kegg_orthology_id": "K02871", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P644", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P644" ], "product": "50S ribosomal protein L13" }, "genes": [], "id": "OAIKOHHJFB_455", "locus": "GCA000209735_00425" }, { "type": "cds", "sequence": "contig_1", "start": 230676, "stop": 231095, "strand": "+", "frame": 3, "gene": "rpsI", "product": "30S ribosomal protein S9", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02996", "RefSeq:WP_013354416.1", "SO:0001217", "UniParc:UPI0001A8C0DC", "UniRef:UniRef100_C4XEB8", "UniRef:UniRef50_Q8R7Y9", "UniRef:UniRef90_C4XEB8" ], "nt": "ATGGCTACAGCTAAAAAAGTTACAAAAAAAGAAGTTGTTGAATACAGAGGACTTGGTCGTCGTAAATCATCAACAGCTCGTGTTATTTTGAGACCTGGAAAAGGTAAATTCACAATTAACAAACGTGAAGCTCTTGCATACTTAACATCAAATTTATATATTCAAGATGCTGAACAACCTTTAGTTTTAACAGAAACAAAAGGACAATTCGATATAACTGTTAATGTTTCAGGTGGTGGTTTAAGTGGACAAGCTGGTGCTATCCGTTTAGGTATTGCTCGTGCTTTATTAGCAGCTTCAGATACATATAGAGAAAAATTGAAACCTGCAGGAATGTTAACTCGTGATGCGCGTGTTACAGAACGTAAAAAACCAGGTTTGAACAAAGCACGTCGTGCAAGACAATTCTCAAAACGTTAA", "aa": "MATAKKVTKKEVVEYRGLGRRKSSTARVILRPGKGKFTINKREALAYLTSNLYIQDAEQPLVLTETKGQFDITVNVSGGGLSGQAGAIRLGIARALLAASDTYREKLKPAGMLTRDARVTERKKPGLNKARRARQFSKR", "aa_hexdigest": "6865a3611ec67d39576e69dc1c2da313", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001A8C0DC", "ncbi_nrp_id": "WP_013354416.1", "uniref100_id": "UniRef100_C4XEB8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C0DC", "RefSeq:WP_013354416.1", "UniRef:UniRef100_C4XEB8" ] }, "ips": { "uniref100_id": "UniRef100_C4XEB8", "uniref90_id": "UniRef90_C4XEB8" }, "psc": { "uniref90_id": "UniRef90_C4XEB8", "gene": "rpsI", "product": "30S ribosomal protein S9", "uniref50_id": "UniRef50_Q8R7Y9", "kegg_orthology_id": "K02996", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q8R7Y9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8R7Y9" ], "product": "Small ribosomal subunit protein uS9" }, "genes": [ "rpsI" ], "id": "OAIKOHHJFB_456", "locus": "GCA000209735_00426" }, { "type": "tRNA", "sequence": "contig_1", "start": 231152, "stop": 231228, "strand": "+", "gene": "trnI", "product": "tRNA-Ile(gat)", "amino_acid": "Ile", "anti_codon": "gat", "score": 82.2, "nt": "GCGAGCGTAGCTCAGCTGGTTAGAGCACACGACTGATAATCGTGAGGTCGATGGTTCAAGTCCATTCGTTCGCACCA", "db_xrefs": [ "SO:0000263" ], "anti_codon_pos": [ 231186, 231188 ], "id": "OAIKOHHJFB_2", "locus": "GCA000209735_00427" }, { "type": "cds", "sequence": "contig_1", "start": 231380, "stop": 231490, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGATCAATTACCTTATTTGCCAAAAAATTTAAAAAGAAGACAACAAAAAAGTTTTGAATTATTAAAAGTTTTAGACAAAATTTGCCGTGAAAACAATTTGTGA", "aa": "MKKDQLPYLPKNLKRRQQKSFELLKVLDKICRENNL", "aa_hexdigest": "00fb306c34e80ce74b54c09c2d458fbd", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4429.2621, "isoelectric_point": 10.01279430389404 }, "id": "OAIKOHHJFB_457", "locus": "GCA000209735_00428" }, { "type": "cds", "sequence": "contig_1", "start": 231860, "stop": 232087, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTATTTTTAGTACCACCTTTAGATATTAAAACAACTATTGAAAAAACTAAGGTTTCAGAAAAAGAAAAAGAAATGTATTACATTACTAATTGGCCTAATAAAAGAATTTTTCATCAAGTTACTTTAACACTAGAACAAATTGAAAATGGCATGCAAGATATAGAAATGAATGGACATTATTTCAAAAGTTTAAAAGAAAATGAATCAGTTCTTGAAAGATGA", "aa": "MFLFLVPPLDIKTTIEKTKVSEKEKEMYYITNWPNKRIFHQVTLTLEQIENGMQDIEMNGHYFKSLKENESVLER", "aa_hexdigest": "cf17c2726fbf78c83a8070c50a237ccf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9036.344099999998, "isoelectric_point": 5.637996101379394 }, "id": "OAIKOHHJFB_458", "locus": "GCA000209735_00429" }, { "type": "tRNA", "sequence": "contig_1", "start": 232270, "stop": 232353, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(caa)", "amino_acid": "Leu", "anti_codon": "caa", "score": 67.2, "nt": "GCCCAAGTGGTGGAATGGTAGACGCTGTGGACTCAAAATCCACTGGAGAGATCCGTGCAGGTTCAAGTCCTGTCTTGGGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 232348, 232350 ], "id": "OAIKOHHJFB_24", "locus": "GCA000209735_00430" }, { "type": "rRNA", "sequence": "contig_1", "start": 232427, "stop": 232533, "strand": "-", "gene": "rrf", "product": "5S ribosomal RNA", "coverage": 0.8991596638655462, "score": 69.1, "evalue": 1.5e-11, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00001", "KEGG:K01985", "SO:0000652" ], "nt": "TCCAGTGGTTATAGCGAAGGGGACCCACCTGATTCCATTCCGAACTCAGTAGTTAAGCCCTTTAGTGCCGACGATACCAGAGATGGGAAAATAGGGAGCTGCTATTT", "id": "OAIKOHHJFB_40", "locus": "GCA000209735_00431" }, { "type": "cds", "sequence": "contig_1", "start": 232894, "stop": 233127, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTATTAAAATTAGTGGTTTTATTTTTGGACCTTTAATTGGTGGAATTGTTGGACTAATTAGTGATCTTTTATCCTTCCTTTTTGTTCCTTCTTTTTATAATCCTATTTATACTGTAGCAGCAATGGTCGATGGAATAATTTCTGGTTTAGTCGGCTTATTATTTATTAAATTATTTGGTTACTATTTTGGTGGTGAATTTCAAGATAATGTTCATGAAAATAGAATTTGA", "aa": "MPIKISGFIFGPLIGGIVGLISDLLSFLFVPSFYNPIYTVAAMVDGIISGLVGLLFIKLFGYYFGGEFQDNVHENRI", "aa_hexdigest": "82836e21f88382467fca2d6588f01146", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8433.870399999996, "isoelectric_point": 4.8263353347778315 }, "id": "OAIKOHHJFB_459", "locus": "GCA000209735_00432" }, { "type": "cds", "sequence": "contig_1", "start": 233401, "stop": 233631, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTATCTGGTTACACATTAATGTTCTTCTTTATTATAATAGCTCGCTTTAAAATGAAACCATCTAACTATTTAGTTATTGTTCCAATTGTTATTTTTTCTGCTTTAATTGAATTAATTAATGTGCCTCTTTTATCATTTGCTGACCAACAATCATTGCAAAAAGGAGAAGATAATAATATCTTTGTATTTGTTTTCAACCATATTTTATTTAGCCCTGTTAAGATTTGA", "aa": "MVSGYTLMFFFIIIARFKMKPSNYLVIVPIVIFSALIELINVPLLSFADQQSLQKGEDNNIFVFVFNHILFSPVKI", "aa_hexdigest": "004f4485996cecc998465410b5c0913e", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8741.412499999995, "isoelectric_point": 8.120389366149901 }, "id": "OAIKOHHJFB_460", "locus": "GCA000209735_00433" }, { "type": "cds", "sequence": "contig_1", "start": 234875, "stop": 234991, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACAATGTTAAGTATTGCTCTTGAATATATTTCTGAAAAAAGTAAAGATGGTAAGAATATCAAATTCAATGATATTTTTAAAGAAATCAAAGCAAATTTAAATGACAAATGA", "aa": "MKTMLSIALEYISEKSKDGKNIKFNDIFKEIKANLNDK", "aa_hexdigest": "15155089558628a0506e499f424d0955", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4419.124800000002, "isoelectric_point": 9.100760078430177 }, "id": "OAIKOHHJFB_461", "locus": "GCA000209735_00434" }, { "type": "cds", "sequence": "contig_1", "start": 235168, "stop": 235257, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAGTAAATGCATCAAGTATGCTTAAAAAAGCTAAAAAAGGAAAGTACGCAGTTCCTCACATTAATATTAACAATTTAGAATGA", "aa": "MKLVNASSMLKKAKKGKYAVPHININNLE", "aa_hexdigest": "63c020b77579d9ad77c92bd5bc852cc4", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3240.8828000000003, "isoelectric_point": 10.12516269683838 }, "id": "OAIKOHHJFB_462", "locus": "GCA000209735_00435" }, { "type": "cds", "sequence": "contig_1", "start": 235336, "stop": 235722, "strand": "+", "frame": 1, "gene": null, "product": "class II fructose-bisphosphate aldolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035C6842C" ], "nt": "ATGGGAGGTATGAATATCATCGTTGGAATGATTAATGGTATGATGAAAGACTACAATATCACAGTTCCAGTTGCACTTCACTTAGACCATGGAACATATGAAGGAGCTAAAAAAGCTATTAAAGCTGGATACACTTCATTAATGTTTGATGGTTCACACTTACCATATGAAGAAAACTTAGCTAAAACAAAAGAATTAGTTAAATTAGCTAAAAAAGTTAACATGTCAATGGAAGCCGAAGTTGGAACAATCGGTGGTGAAGAAGATGGTATTATCGGTGCAGGCGAATTTGCTGATCCAGCTGAAGCTAAAGAAATGGCTGAAACAGGTATTGATGTTTTAGCAGCTGGTATTGGTAACATTCATGGTCCATATCCTATAAGCTGA", "aa": "MGGMNIIVGMINGMMKDYNITVPVALHLDHGTYEGAKKAIKAGYTSLMFDGSHLPYEENLAKTKELVKLAKKVNMSMEAEVGTIGGEEDGIIGAGEFADPAEAKEMAETGIDVLAAGIGNIHGPYPIS", "aa_hexdigest": "b8ece84c05e30ae815d3fcafb7083b00", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0015C42C51", "query_cov": 0.9921875, "subject_cov": 0.8523489932885906, "identity": 0.504, "score": 132.0, "evalue": 4.97e-37, "valid": false, "product": "class II fructose-bisphosphate aldolase", "ec_ids": [ "4.1.2.13" ], "uniref50_id": "UniRef50_UPI0035C6842C", "kegg_orthology_id": "K01624" }, "pscc": { "uniref50_id": "UniRef50_UPI0035C6842C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035C6842C" ], "product": "class II fructose-bisphosphate aldolase" }, "genes": [], "id": "OAIKOHHJFB_463", "locus": "GCA000209735_00436" }, { "type": "cds", "sequence": "contig_1", "start": 236258, "stop": 236461, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00565.23" ], "nt": "TTGTATTTTACCAATGATACTATAAAACAAGGTGTTAGATTTTTTGGAATAGATACTCCAGAGATATTTAAAGGCAAAAATAATCAACATTTAGCTTTGCTTGAAAACTATTATGGACAAAAAGCAAAAAATTATTTATACAATTTAACATATCAAAAAAATATAAAAATTAAATACATAACGAAGGATAAATATAATCGGTGA", "aa": "MYFTNDTIKQGVRFFGIDTPEIFKGKNNQHLALLENYYGQKAKNYLYNLTYQKNIKIKYITKDKYNR", "aa_hexdigest": "39ea9fe130d16a562402197c5ffa17dd", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00565.23", "name": "SNase", "length": 56, "aa_cov": 0.835820895522388, "hmm_cov": 0.8928571428571429, "evalue": 1.2065131256201743e-09, "score": 34.69647979736328, "start": 12, "stop": 67 } ], "seq_stats": { "molecular_weight": 8120.213899999999, "isoelectric_point": 9.698124122619632 }, "id": "OAIKOHHJFB_464", "locus": "GCA000209735_00437" }, { "type": "cds", "sequence": "contig_1", "start": 236876, "stop": 237811, "strand": "-", "frame": 1, "gene": "pyk", "product": "pyruvate kinase", "db_xrefs": [ "EC:2.7.1.40", "GO:0000287", "GO:0004743", "GO:0006096", "GO:0030955", "KEGG:K00873", "SO:0001217", "UniRef:UniRef50_S5M1G4", "UniRef:UniRef90_A0A3P8K9P9" ], "nt": "ATGGCTCAAGACTTAGTTGTTGGAAACCAAGTTCTCTTTGACGATGGTAAATTATCAACAATTGTAACTAAAGTTGATAGAAAAGCTGGTTATGTTGTTGTTAAAACACTTAACAAGCACTTATTAAAAACTAACAAACGTATTAATTTACCAGGTGTTGACTTTTCTCTTCCATTTTTAGCTCAAAAAGACATTGATGATGTTAAATTTGGTATTAAAAACGGAATTAACTATGTAGCAGCATCATTTGTTAACTCATCTAAAAATGTCCAAGAATTACGTAAATTATTAGATGACAATGGTGGAAAACACGTTAAAATCATTTCTAAAATTGAAAGCCACTTAGGAACCAAAAACATTGATGAAATCATTCAAGCATCAGATGGTGTTATGGTTGCTCGTGGGGACTTAGGTCTTGAAATTCCTTACTATGATGTTCCTTATTTTGAAAAAATAATGATCAGAAAATGTCGTGAAGCGGGTAAACCAGTTGTTGTTGCAACTCAAATGTTAGACTCAATGGAAAACTCACCACAACCTACACGTGCTGAAGTTACTGACGTTTACTATGCAACAGAAATGGGTGCTGACTCAACAATGCTTTCAGGTGAAAGTGCATCAGGCAACTATCCTTTAGAAGCAGTTACAGTTATGTCAAAAATTAACAAACGTGCTGAAAGTGAATTTTATGACAAAAATTACTATGATGTTCAATTAGAAGAAATTAGAAAAAATTCTTCAAATTCAAAACGTTCAAGAATTGCTTATGACGTTGCAAACAGAACACGTTATGGTGACTATAGATTTGCTGTTGTTCTTTCAAGAACAGGTCAATTATTAAGTGAAGTTGCTAAATTTAGACCAAATACATTAATCGTTGGTATTTTAAATGATGAAAACCTAATCGGTGGATTTGGTGTTACTTCTAGTGTTTGA", "aa": "MAQDLVVGNQVLFDDGKLSTIVTKVDRKAGYVVVKTLNKHLLKTNKRINLPGVDFSLPFLAQKDIDDVKFGIKNGINYVAASFVNSSKNVQELRKLLDDNGGKHVKIISKIESHLGTKNIDEIIQASDGVMVARGDLGLEIPYYDVPYFEKIMIRKCREAGKPVVVATQMLDSMENSPQPTRAEVTDVYYATEMGADSTMLSGESASGNYPLEAVTVMSKINKRAESEFYDKNYYDVQLEEIRKNSSNSKRSRIAYDVANRTRYGDYRFAVVLSRTGQLLSEVAKFRPNTLIVGILNDENLIGGFGVTSSV", "aa_hexdigest": "1f4f1a05519381d88e406f276f439efe", "start_type": "ATG", "rbs_motif": null, "genes": [ "pyk" ], "truncated": "both", "pseudogene": { "start": 236732, "stop": 238111, "observations": { "mutation": [ 236878, 237877 ], "stop": [ 236878, 237877 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8K9P9", "query_cov": 1.0, "subject_cov": 0.6519916142557652, "identity": 1.0, "score": 601.0, "evalue": 2.09e-214, "gene_start": 1, "gene_end": 311, "reference_start": 119, "reference_end": 429, "reference_sequence": "MNITDKRSKLVITVGPSSDNYKMLRSLIEAGATCVRANFSHGDHAEQLNKFVLAQKISKDLHLPISLMLDTKGPEIRLGKMKDGKQEIPANKEIKVWTTKEKYETVEGTEKELTVAYDMAQDLVVGNQVLFDDGKLSTIVTKVDRKAGYVVVKTLNKHLLKTNKRINLPGVDFSLPFLAQKDIDDVKFGIKNGINYVAASFVNSSKNVQELRKLLDDNGGKHVKIISKIESHLGTKNIDEIIQASDGVMVARGDLGLEIPYYDVPYFEKIMIRKCREAGKPVVVATQMLDSMENSPQPTRAEVTDVYYATEMGADSTMLSGESASGNYPLEAVTVMSKINKRAESEFYDKNYYDVQLEEIRKNSSNSKRSRIAYDVANRTRYGDYRFAVVLSRTGQLLSEVAKFRPNTLIVGILNDENLIGGFGVTSSVWVSLDSIELFAKIKKDKSKALNALKPYGVKKGDRFLVVENESITEFVA" }, "paralog": false, "identity": 0.9956427015250545, "score": 888.0, "evalue": 0.0, "description": "Internal stop codon at 236878, 237877. Nonsense mutation around 236878, 237877.", "psc": { "uniref90_id": "UniRef90_A0A3P8K9P9", "gene": "pyk", "product": "pyruvate kinase", "ec_ids": [ "2.7.1.40" ], "uniref50_id": "UniRef50_S5M1G4", "kegg_orthology_id": "K00873", "go_ids": [ "GO:0000287", "GO:0004743", "GO:0006096", "GO:0030955" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8K9P9", "EC:2.7.1.40", "UniRef:UniRef50_S5M1G4", "KEGG:K00873", "GO:0000287", "GO:0004743", "GO:0006096", "GO:0030955" ] }, "pscc": { "uniref50_id": "UniRef50_S5M1G4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_S5M1G4" ], "product": "Pyruvate kinase" } }, "id": "OAIKOHHJFB_465", "locus": "GCA000209735_00438" }, { "type": "cds", "sequence": "contig_1", "start": 237875, "stop": 238165, "strand": "-", "frame": 1, "gene": null, "product": "pyruvate kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B60A9" ], "nt": "ATGAACATTACTGACAAAAGAAGTAAGTTAGTTATTACAGTTGGTCCATCAAGTGATAACTACAAAATGCTTCGTTCATTAATTGAAGCAGGTGCAACCTGTGTTCGTGCTAACTTTAGCCACGGAGACCATGCAGAACAATTAAACAAATTTGTTCTTGCACAAAAAATTTCTAAAGATTTACATCTTCCTATATCTCTTATGTTAGATACTAAGGGTCCGGAAATTCGTTTAGGAAAAATGAAAGATGGAAAACAAGAAATTCCAGCAAACAAAGAAATTAAAGTTTGA", "aa": "MNITDKRSKLVITVGPSSDNYKMLRSLIEAGATCVRANFSHGDHAEQLNKFVLAQKISKDLHLPISLMLDTKGPEIRLGKMKDGKQEIPANKEIKV", "aa_hexdigest": "d00046a4307e35aa0ac1a254290185b4", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI00387B60A9", "query_cov": 0.8645833333333334, "subject_cov": 0.9540229885057471, "identity": 0.711, "score": 125.0, "evalue": 8.67e-36, "valid": false, "product": "pyruvate kinase", "ec_ids": [ "2.7.1.40" ], "uniref50_id": "UniRef50_UPI00387B60A9", "kegg_orthology_id": "K00873" }, "pscc": { "uniref50_id": "UniRef50_UPI00387B60A9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B60A9" ], "product": "pyruvate kinase" }, "genes": [], "id": "OAIKOHHJFB_466", "locus": "GCA000209735_00439" }, { "type": "cds", "sequence": "contig_1", "start": 238349, "stop": 238597, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATTTAAAGATGATTTTAAAAATAGTCACCTTTACCAATTATTCTATTGTACAAAACAAACAGAAGATTATGATAAAGACAAAACAAAATATAAAAATGAAGGTTTAGTTTCATTTGATCCAGCAAAAGGCGAAGCTACCTTTGCAATTGGTATTAGAGTTAAAAATTCAAATAATAATTATAAGTCTTCTTCAATTCCTGAATATATTGGACATATAACTTTAGGTGGTTTTAAAAAATCATAA", "aa": "MKFKDDFKNSHLYQLFYCTKQTEDYDKDKTKYKNEGLVSFDPAKGEATFAIGIRVKNSNNNYKSSSIPEYIGHITLGGFKKS", "aa_hexdigest": "ba99a65ba617ad9e85d4b70fba226503", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9440.507699999998, "isoelectric_point": 9.16303653717041 }, "id": "OAIKOHHJFB_467", "locus": "GCA000209735_00440" }, { "type": "cds", "sequence": "contig_1", "start": 238667, "stop": 238864, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAAGGACAAAAATGATAAAACATCTTTAATAAAATCAAGAATGATTGACATAAAAAATCCTATTGTTCAAGAAATAGATATTAATTATTTTGAACTTGAAAAAGTATTGAAAAATCATGTTGAAATATCTATTTTAGGTAACTATGAAATTAAAAATATTGACTTTAAAACACCTTCAACTTCACTTGAATGA", "aa": "MPKDKNDKTSLIKSRMIDIKNPIVQEIDINYFELEKVLKNHVEISILGNYEIKNIDFKTPSTSLE", "aa_hexdigest": "56d1e0ee84b5825f31a63661b8e70e94", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7592.694899999999, "isoelectric_point": 5.780320930480956 }, "id": "OAIKOHHJFB_468", "locus": "GCA000209735_00441" }, { "type": "cds", "sequence": "contig_1", "start": 239009, "stop": 239164, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATATTAGAGATAAAATAAGACAAACTTTTTACAAGAAAGAAAAGAGTAGTCTTGATGAATTACTTGCTGCATTACCACAAAAAGATCTTTCAAGTGATTTAAACAAAAATAAAGAAGAAATTTTAAAAAATAATTCAACAATTGAAAGTTGA", "aa": "MDIRDKIRQTFYKKEKSSLDELLAALPQKDLSSDLNKNKEEILKNNSTIES", "aa_hexdigest": "2fa731d2308752bb98229033ee3aa0b9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5939.660099999998, "isoelectric_point": 6.175920295715332 }, "id": "OAIKOHHJFB_469", "locus": "GCA000209735_00442" }, { "type": "cds", "sequence": "contig_1", "start": 239264, "stop": 239464, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGAAATCAATTATTTTCATTAAGAAATAATCGTTTTGATAGAATTGACAAATTTTATAAAGATTACAATATTTTCTACCATTCATATGCAAATGATTTTACAGGTACACTCTATTTGACTGTTTCATTAGAAGATAAAGCTAATGAAAAAGATAAAGATAAAAAAACAAGAAACATTAGTGATAGAATAAAAAACTGA", "aa": "MRNQLFSLRNNRFDRIDKFYKDYNIFYHSYANDFTGTLYLTVSLEDKANEKDKDKKTRNISDRIKN", "aa_hexdigest": "70cab7cf96435a1a43b11fa1e643cbbd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8089.936699999999, "isoelectric_point": 9.474483299255372 }, "id": "OAIKOHHJFB_470", "locus": "GCA000209735_00443" }, { "type": "cds", "sequence": "contig_1", "start": 239534, "stop": 239830, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCGTAAATTATCATTAACATTAGGAACATTAGCAACAGGTGTTGTTGCAGTTGGAATTGGTACGGCTATTTATGCTTTAGTTAAAACTAATAAAGACAATAAAAGTATTGAAAGACAGCTAAGCTCTTTTACTTTTTCTCTTGCAAAACCTAAAGACAAATTATACCAAACTAATATTGCAAATCACTTAGCAAGTGAATTTACTTACTCAAATTATTACTATTTTGAAACATACAATAGCAACAAAGAACCAACTTTGAAAAACACAAAAGAATATGAAAAAAATTATTGA", "aa": "MNRKLSLTLGTLATGVVAVGIGTAIYALVKTNKDNKSIERQLSSFTFSLAKPKDKLYQTNIANHLASEFTYSNYYYFETYNSNKEPTLKNTKEYEKNY", "aa_hexdigest": "a5046db0ae9ce25ddb62516bcc737bcb", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11177.487099999991, "isoelectric_point": 9.397894859313965 }, "id": "OAIKOHHJFB_471", "locus": "GCA000209735_00444" }, { "type": "cds", "sequence": "contig_1", "start": 240085, "stop": 240216, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCTTGCGGTTAAAGATTTTTTAAAAAATGAACCTTCTCCAAAACATATCTTTTTGCTTATAGATAGTCGAACAAGATTTACTAAAAATGATTTAGAAACCTTGACATTTTTAAATTCATTAAACTGA", "aa": "MNLAVKDFLKNEPSPKHIFLLIDSRTRFTKNDLETLTFLNSLN", "aa_hexdigest": "9a7b4fa6c0f92d811c625176db386597", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5035.772399999999, "isoelectric_point": 8.211225318908692 }, "id": "OAIKOHHJFB_472", "locus": "GCA000209735_00445" }, { "type": "cds", "sequence": "contig_1", "start": 240477, "stop": 240905, "strand": "-", "frame": 3, "gene": "coaD", "product": "pantetheine-phosphate adenylyltransferase", "db_xrefs": [ "EC:2.7.7.3", "GO:0004595", "GO:0015937", "KEGG:K00954", "RefSeq:WP_013526711.1", "SO:0001217", "UniParc:UPI0001F59FC9", "UniRef:UniRef100_C4XEC9", "UniRef:UniRef50_A0A449A8S0", "UniRef:UniRef90_C4XEC9" ], "nt": "ATGATTAAAAAAGCAATTTTTCCAGGATCTTTTGATCCTATACACAAAGGTCATATATCAGTAATTGAAAAAGCACTAAAGCTTTTTGATGAGCTTATAGTAATAGTTTCAATTAACCCTGATAAAAACAATTTAGGTAATTTAGAAGAAAGATATCAATCAGTCAAAAAAACCTTAAAAGACTATAAAACAGTCACTGTGGTAAAAAATGAAAATGATTTGATAGCTCAAATTGCTAAAGAAAAAAATGTAAAGTTCCTAGTTAGATCAGCCAGAAATAATTTAGATTATGACTATGAGCTAGTACTAGCAGCTGCTAATCATCACTTAAATTCTGAGCTTGAAACTATACTTATAGTACCAGACTATGAAAACATAGACTATTCTTCAACTCTTTTAAGACATAAACAAAAATTAGGTAAAAAATAA", "aa": "MIKKAIFPGSFDPIHKGHISVIEKALKLFDELIVIVSINPDKNNLGNLEERYQSVKKTLKDYKTVTVVKNENDLIAQIAKEKNVKFLVRSARNNLDYDYELVLAAANHHLNSELETILIVPDYENIDYSSTLLRHKQKLGKK", "aa_hexdigest": "087e78ccb649164a0c260c2e363af15b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59FC9", "ncbi_nrp_id": "WP_013526711.1", "uniref100_id": "UniRef100_C4XEC9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59FC9", "RefSeq:WP_013526711.1", "UniRef:UniRef100_C4XEC9" ] }, "ips": { "uniref100_id": "UniRef100_C4XEC9", "uniref90_id": "UniRef90_C4XEC9" }, "psc": { "uniref90_id": "UniRef90_C4XEC9", "gene": "coaD", "product": "pantetheine-phosphate adenylyltransferase", "ec_ids": [ "2.7.7.3" ], "uniref50_id": "UniRef50_A0A449A8S0", "kegg_orthology_id": "K00954", "go_ids": [ "GO:0004595", "GO:0015937" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449A8S0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A8S0" ], "product": "Phosphopantetheine adenylyltransferase" }, "genes": [ "coaD" ], "id": "OAIKOHHJFB_473", "locus": "GCA000209735_00446" }, { "type": "cds", "sequence": "contig_1", "start": 240908, "stop": 241426, "strand": "-", "frame": 1, "gene": null, "product": "Acetate kinase", "db_xrefs": [ "EC:2.7.2.1", "KEGG:K00925", "SO:0001217", "UniRef:UniRef50_L0RV90", "UniRef:UniRef90_C4XED0" ], "nt": "ATGGGACTTACACCTTTAGCAGGAATTATGATGGGATCGCGTTCAGGCGATATTGATCCTTCAATTCATGAATTTATTAATGAACAACTTGGTTTAGATATTCACCAAATCACAGAAGCTCTTAACAAGAAAAGTGGGCTTTTAGGAGTTTCAGGTGTTTCAAGCGATATGCGTGATGTATGTAAAGCAGCAGACGAAGGCAATAAACGTGCTATTTTCACACTTGAATTGTATGCACAAAAAATAGCTGATTTTACCGCTAATTATGCAAACAAAATTGGCAACAAATTAGATGCTATTGTTTTCACTGCTGGTGTTGGTGAAAACACTCCTTCAATGCGTCAAATGGTAGTTGATAAACTACATTTTGCAAAGATTAAATTAGATGACAAAAAGAATAATTCTAAAATTGAAGATTATGCTTTAATTTCTACAAAAGATAGCGAAATCAAAGTATATGTAATTAGAACAAATGAAGAATTATTAATTGCAAAACATGCAATAAATTTGTATAAATAA", "aa": "MGLTPLAGIMMGSRSGDIDPSIHEFINEQLGLDIHQITEALNKKSGLLGVSGVSSDMRDVCKAADEGNKRAIFTLELYAQKIADFTANYANKIGNKLDAIVFTAGVGENTPSMRQMVVDKLHFAKIKLDDKKNNSKIEDYALISTKDSEIKVYVIRTNEELLIAKHAINLYK", "aa_hexdigest": "43a499d540fc49d4941e831af02a5074", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_R6YLS3", "query_cov": 0.9651162790697675, "subject_cov": 0.8383838383838383, "identity": 0.518, "score": 145.0, "evalue": 5.34e-41, "valid": false, "product": "Acetate kinase", "ec_ids": [ "2.7.2.1" ], "uniref50_id": "UniRef50_R6YLS3", "kegg_orthology_id": "K00925" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 240908, "stop": 241726, "observations": { "mutation": [ 241600 ], "stop": [ 241600 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XED0", "query_cov": 1.0, "subject_cov": 0.4321608040201005, "identity": 1.0, "score": 333.0, "evalue": 2.17e-112, "gene_start": 1, "gene_end": 172, "reference_start": 227, "reference_end": 398, "reference_sequence": "MMSKQKVLVINAGSSSIKLSLFDKASLEIIASGIAERITLLEGKITIKYNGQKFNRDVTMPNHTVAVENILELMSQIGLIDNPKEIELIGFRVVHGGPYFTTTVKLNTKEIDLIEKAKIYAPLHNPGAVQAMKAFKKVMPHAKMSVDFDTAFHTTIPDVNAIYPIPYQWTEDLKIRRYGAHGISHEFITLKIQELVKKEKVNLVNLHIGNGASLCAIKDRKSFDTSMGLTPLAGIMMGSRSGDIDPSIHEFINEQLGLDIHQITEALNKKSGLLGVSGVSSDMRDVCKAADEGNKRAIFTLELYAQKIADFTANYANKIGNKLDAIVFTAGVGENTPSMRQMVVDKLHFAKIKLDDKKNNSKIEDYALISTKDSEIKVYVIRTNEELLIAKHAINLYK" }, "paralog": false, "identity": 0.9963235294117647, "score": 533.0, "evalue": 2.59e-193, "description": "Internal stop codon at 241600. Nonsense mutation around 241600.", "psc": { "uniref90_id": "UniRef90_C4XED0", "product": "Acetate kinase", "ec_ids": [ "2.7.2.1" ], "uniref50_id": "UniRef50_L0RV90", "kegg_orthology_id": "K00925", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XED0", "EC:2.7.2.1", "UniRef:UniRef50_L0RV90", "KEGG:K00925" ] }, "pscc": { "uniref50_id": "UniRef50_L0RV90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RV90" ], "product": "Acetate kinase" } }, "id": "OAIKOHHJFB_474", "locus": "GCA000209735_00447" }, { "type": "cds", "sequence": "contig_1", "start": 241598, "stop": 242101, "strand": "-", "frame": 1, "gene": null, "product": "Acetate kinase", "db_xrefs": [ "EC:2.7.2.1", "KEGG:K00925", "SO:0001217", "UniRef:UniRef50_L0RV90", "UniRef:UniRef50_UPI0028BDD23C", "UniRef:UniRef90_C4XED0" ], "nt": "ATGAGTAAGCAAAAAGTATTAGTTATTAATGCAGGAAGTAGTTCAATAAAGTTATCACTTTTTGATAAAGCTTCTTTAGAAATTATTGCAAGTGGTATTGCAGAGAGGATTACCTTATTAGAAGGTAAAATAACAATCAAATATAACGGTCAAAAATTTAACCGTGATGTAACAATGCCTAACCACACTGTGGCTGTTGAAAACATTTTAGAATTAATGTCACAAATTGGTTTAATTGACAATCCAAAAGAAATCGAATTAATTGGCTTTAGAGTTGTTCATGGTGGACCTTACTTTACAACAACAGTTAAATTGAATACTAAAGAAATTGATTTAATTGAAAAAGCTAAAATTTATGCACCACTTCACAATCCAGGTGCAGTTCAAGCCATGAAAGCATTTAAAAAAGTTATGCCACATGCCAAAATGAGTGTTGACTTTGATACAGCATTCCACACAACAATTCCAGATGTCAATGCAATTTATCCAATTCCATATCAATGA", "aa": "MSKQKVLVINAGSSSIKLSLFDKASLEIIASGIAERITLLEGKITIKYNGQKFNRDVTMPNHTVAVENILELMSQIGLIDNPKEIELIGFRVVHGGPYFTTTVKLNTKEIDLIEKAKIYAPLHNPGAVQAMKAFKKVMPHAKMSVDFDTAFHTTIPDVNAIYPIPYQ", "aa_hexdigest": "b3d35d81c65fe847b2a9740e420e5e5e", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0021AE5BBA", "query_cov": 0.9640718562874252, "subject_cov": 0.8256410256410256, "identity": 0.609, "score": 192.0, "evalue": 9.47e-60, "valid": false, "product": "Acetokinase family", "uniref50_id": "UniRef50_UPI0028BDD23C" }, "pscc": { "uniref50_id": "UniRef50_UPI0028BDD23C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0028BDD23C" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 241295, "stop": 242104, "observations": { "mutation": [ 241600 ], "stop": [ 241600 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XED0", "query_cov": 1.0, "subject_cov": 0.41959798994974873, "identity": 1.0, "score": 327.0, "evalue": 9.51e-110, "gene_start": 1, "gene_end": 167, "reference_start": 2, "reference_end": 168, "reference_sequence": "MMSKQKVLVINAGSSSIKLSLFDKASLEIIASGIAERITLLEGKITIKYNGQKFNRDVTMPNHTVAVENILELMSQIGLIDNPKEIELIGFRVVHGGPYFTTTVKLNTKEIDLIEKAKIYAPLHNPGAVQAMKAFKKVMPHAKMSVDFDTAFHTTIPDVNAIYPIPYQWTEDLKIRRYGAHGISHEFITLKIQELVKKEKVNLVNLHIGNGASLCAIKDRKSFDTSMGLTPLAGIMMGSRSGDIDPSIHEFINEQLGLDIHQITEALNKKSGLLGVSGVSSDMRDVCKAADEGNKRAIFTLELYAQKIADFTANYANKIGNKLDAIVFTAGVGENTPSMRQMVVDKLHFAKIKLDDKKNNSKIEDYALISTKDSEIKVYVIRTNEELLIAKHAINLYK" }, "paralog": false, "identity": 0.9925650557620818, "score": 525.0, "evalue": 4.74e-190, "description": "Internal stop codon at 241600. Nonsense mutation around 241600.", "psc": { "uniref90_id": "UniRef90_C4XED0", "product": "Acetate kinase", "ec_ids": [ "2.7.2.1" ], "uniref50_id": "UniRef50_L0RV90", "kegg_orthology_id": "K00925", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XED0", "EC:2.7.2.1", "UniRef:UniRef50_L0RV90", "KEGG:K00925" ] }, "pscc": { "uniref50_id": "UniRef50_L0RV90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RV90" ], "product": "Acetate kinase" } }, "id": "OAIKOHHJFB_475", "locus": "GCA000209735_00448" }, { "type": "cds", "sequence": "contig_1", "start": 242111, "stop": 243064, "strand": "-", "frame": 1, "gene": null, "product": "Phosphate acetyl/butaryl transferase domain-containing protein", "db_xrefs": [ "RefSeq:WP_013526713.1", "SO:0001217", "UniParc:UPI0001A8C0E9", "UniRef:UniRef100_C4XED1", "UniRef:UniRef50_Q4A5B1", "UniRef:UniRef90_C4XED1" ], "nt": "ATGAAATTTACATCTTTAATTGAAAAAAGAATTAAAGCAATGAAAACTAAAAAAAGAATTGCTTTTATAGATGGTGATGATAGTAGAGCACTAGATACCATTAAACAATTAGAACAATTTCCTAATGTTGAATTAACTTTATTAGTTGAAACTGATGTTAAGTCAAAAACCAATGTTAAGTTTATAAACATTAATAAAGATAAACAAAAACAAAATGATTTAGCTCAAAAATATTTTGAATTAAGAAAAGGCAAAGAAACAATTGAACAAGCTCAAAGAAACCTAAAAACAAGACCTTTCTATGCTATGATGCTTTTAGCTACAGGTGAAGTTGATGGGGTAGTTGGCGGACTTAATTATGCAACTGCAGATATCTTAAGAGCAGCTTTTAAAGCTATAGGCGCTAGAGAAGGAGTAAAAACTATTTCTTCAGTTATGGTAGTACATAACGAAAAAAATACTTACTTCTTCAGTGACATTTCAGTAAATGTTAAGCCTGATCAAAAAGCTTTAGTAGAAATTGGACTTAATGCTGCTCAATTTGCTCAAAGCATGGGGATAGAACCTAAAATAGCCTTCTTAAGTTTCTCAACTAACTATTCAGCAAAAACACCTGAAAGTGAATTAGTACACAATGCTGTTGAAGAATTCAATACCATTTATCAAGGTACTAAAGCTATTGGAGAAATTCAATTAGATGCTGCTTTAGATTTAGAAGTTAGAAAAGCTAAATATAAATTAGATTACTATAAAGAACCAGCAAATACTTTAATTTTCCCTGATTTAGATGCTGGAAATATAGGCTATAAATTAGCTCAAAGATTAGGAAATTATGGGGCAATTGGACCTATTGTTGTTGGTACTAAAAAACCAGTCAATGATCTTTCAAGAGGTTCAAGTGTAAAAGATGTAATTAATACAATTTTAATCACTGCATTGCAGGCAGAGAAATAG", "aa": "MKFTSLIEKRIKAMKTKKRIAFIDGDDSRALDTIKQLEQFPNVELTLLVETDVKSKTNVKFININKDKQKQNDLAQKYFELRKGKETIEQAQRNLKTRPFYAMMLLATGEVDGVVGGLNYATADILRAAFKAIGAREGVKTISSVMVVHNEKNTYFFSDISVNVKPDQKALVEIGLNAAQFAQSMGIEPKIAFLSFSTNYSAKTPESELVHNAVEEFNTIYQGTKAIGEIQLDAALDLEVRKAKYKLDYYKEPANTLIFPDLDAGNIGYKLAQRLGNYGAIGPIVVGTKKPVNDLSRGSSVKDVINTILITALQAEK", "aa_hexdigest": "42d7ecc2928b49aab7ff94df45fca491", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001A8C0E9", "ncbi_nrp_id": "WP_013526713.1", "uniref100_id": "UniRef100_C4XED1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C0E9", "RefSeq:WP_013526713.1", "UniRef:UniRef100_C4XED1" ] }, "ips": { "uniref100_id": "UniRef100_C4XED1", "uniref90_id": "UniRef90_C4XED1" }, "psc": { "uniref90_id": "UniRef90_C4XED1", "product": "Phosphate acetyl/butaryl transferase domain-containing protein", "uniref50_id": "UniRef50_Q4A5B1" }, "pscc": { "uniref50_id": "UniRef50_Q4A5B1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5B1" ], "product": "Phosphate acetyltransferase" }, "genes": [], "id": "OAIKOHHJFB_476", "locus": "GCA000209735_00449" }, { "type": "cds", "sequence": "contig_1", "start": 243525, "stop": 243779, "strand": "+", "frame": 3, "gene": null, "product": "Transposase", "db_xrefs": [ "RefSeq:WP_013526716.1", "SO:0001217", "UniParc:UPI0001A8C0EB", "UniRef:UniRef100_C4XED3", "UniRef:UniRef50_C4XED3", "UniRef:UniRef90_C4XED3" ], "nt": "ATGGATATTGAAGCTGAAAAAAAGAATAGAGAAGAAGAATTAAGAAAGATGACAAGAGATCAACTTGAAATCTTAATAACTGATATTTCTTACAAAGAAATATTGGAAAGAAATAACAGTAAAGATTTAGATGAAGCAATTGAAAAATTCAAAAAAAGATATGGATTTTCTGTCACCATTAAACAAATTATCGCTATTTTAGGTATTAAAAAATCGACATATTATTATCAATTAAAACAAAGAAAAAAAGATTAA", "aa": "MDIEAEKKNREEELRKMTRDQLEILITDISYKEILERNNSKDLDEAIEKFKKRYGFSVTIKQIIAILGIKKSTYYYQLKQRKKD", "aa_hexdigest": "b784ba13d9bee4bf5f79cb33046cb416", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C0EB", "ncbi_nrp_id": "WP_013526716.1", "uniref100_id": "UniRef100_C4XED3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C0EB", "RefSeq:WP_013526716.1", "UniRef:UniRef100_C4XED3" ] }, "ips": { "uniref100_id": "UniRef100_C4XED3", "uniref90_id": "UniRef90_C4XED3" }, "psc": { "uniref90_id": "UniRef90_C4XED3", "product": "Transposase", "uniref50_id": "UniRef50_C4XED3" }, "pscc": { "uniref50_id": "UniRef50_C4XED3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XED3" ], "product": "Transposase" }, "genes": [], "id": "OAIKOHHJFB_477", "locus": "GCA000209735_00450" }, { "type": "cds", "sequence": "contig_1", "start": 244180, "stop": 244329, "strand": "+", "frame": 1, "gene": null, "product": "IS3 family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0009993DF3" ], "nt": "ATGTCAAGAGTTGGGAATTCATTATATAATAGAGAAATTGAATATTTCTTTTCTATATTAAAAACCGAAATCTTTCCTAATTTTTGCAAAAGAGTTAAGTTGCTCACTTTTAACGAATTGAAAAATGAAATTAAAGAATTTATTGATTGA", "aa": "MSRVGNSLYNREIEYFFSILKTEIFPNFCKRVKLLTFNELKNEIKEFID", "aa_hexdigest": "80df6f3186f2c7c96547632095695c59", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002AFF27A6", "query_cov": 1.0, "subject_cov": 0.8448275862068966, "identity": 0.6940000000000001, "score": 70.5, "evalue": 3.7e-15, "valid": false, "product": "IS3 family transposase", "uniref50_id": "UniRef50_UPI0009993DF3" }, "pscc": { "uniref50_id": "UniRef50_UPI0009993DF3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0009993DF3" ], "product": "IS3 family transposase" }, "genes": [], "id": "OAIKOHHJFB_478", "locus": "GCA000209735_00451" }, { "type": "cds", "sequence": "contig_1", "start": 244679, "stop": 245368, "strand": "-", "frame": 1, "gene": "folD", "product": "Bifunctional protein FolD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RXG1" ], "nt": "ATGAAAATCTTAGATGGAAAAACTCTTGCTCAAAAAAGAACTAAAGAACTCATTGCACAATTTAAGGATATAAAGGAAAAGTTAGGCAGAAAACCAATTTTATCAATTATTCAAGTTGGCGATAATCCCGCATCTACTCACTACATCAAAAACAAATTAAAGAAGGCTGAAGAATTAGGAGTAGAAGCAATTCATCATCATTTTCCAGCTGATATTAGACAAAAAGATTTGCTTAAGAAATTAGACATTATTAATGAAAAATCTGATGGTATTGTTATTCAACTTCCTTTACCTAAAGAATTTGCCACACAAGTTATTTTAGATAGCGTTCGCATTGAAAAGGATATTGATGGTTTATGTACCAGAAATGCATTCAATTTCTACAATCAAGCGGATGACTTTACCTTAGTTCCAGCTACTGCTATTGGTATTTTAGATTTATTAAATAGCTACAATATCAAAATGATTGACAAAAAAGCTGCTGTTATTGGTCGTTCAAGTTTAGTTGGAAAACCTACAGCATTTCTATTAAAACAAGAAGGTTGCAATGTTTCTACCTACAATAGAAACACAGGCATTAAAGGTGTTGAAAATGCTGATATCATTGTAGTTGCTGCTGGTGAAGCCAAACTTCTTAAAAAAGAAAACATTAAAGAAGGTGCTGTTGTAGTTGATGTTGGATCAAACTGA", "aa": "MKILDGKTLAQKRTKELIAQFKDIKEKLGRKPILSIIQVGDNPASTHYIKNKLKKAEELGVEAIHHHFPADIRQKDLLKKLDIINEKSDGIVIQLPLPKEFATQVILDSVRIEKDIDGLCTRNAFNFYNQADDFTLVPATAIGILDLLNSYNIKMIDKKAAVIGRSSLVGKPTAFLLKQEGCNVSTYNRNTGIKGVENADIIVVAAGEAKLLKKENIKEGAVVVDVGSN", "aa_hexdigest": "b0ba4cc88f8b47363debf5a24bf6dbee", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_A0A3P8L7E0", "query_cov": 1.0, "subject_cov": 0.8063380281690141, "identity": 0.873, "score": 403.0, "evalue": 1.38e-140, "valid": false, "product": "Bifunctional protein FolD", "uniref50_id": "UniRef50_L0RXG1" }, "pscc": { "uniref50_id": "UniRef50_L0RXG1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RXG1" ], "product": "Bifunctional protein FolD" }, "genes": [ "folD" ], "id": "OAIKOHHJFB_479", "locus": "GCA000209735_00452" }, { "type": "cds", "sequence": "contig_1", "start": 245365, "stop": 245940, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08264.19", "PFAM:PF10458.14" ], "nt": "ATGCCAAATAAAAAAGCTGCTTTAGATAACCTAAAATCATTACTTTTAATAGCTCATCCATTTTTACCTTTTATTACTGATCACTTATATAAAGTACTATTTAACAAAGAATTATTAGCTCAAGAATTTCCAAACTTAAAGAATCAAAAAGTTGAAAATCTTGAACAAATTGAACAAATAATTTCTATTGTAAATATACTTAGAAAATATCGTGAAGACAATAATATTAGTAAAAAAGAAGTGCTTTATTATGACACTAATATTAAACAAAATGAGTTTGTTAAAAATTCACTAAAATCACTATCAAACAGTGAAATTAGTGAAAATAAAGACACTTTATTTGTTGATGGAAATATTAATGTTTATATTAAAGAATCAGCCGAGCAAAAAAACACTTACATTGCTAAATTAAAAGAGAAAATAGCTAATTTAGAAAGCGAAGTTAAACGTTCAAACAATATTTTAAGTAATCAAAACTTTATAGCTAAAGCACCTACATCTAAAGTTGAAGAAGAAAAACAAAAACTAGCTAAATATCAAGAAGAATTAGACAAATACAAGGAGGAGTTAAAATGA", "aa": "MPNKKAALDNLKSLLLIAHPFLPFITDHLYKVLFNKELLAQEFPNLKNQKVENLEQIEQIISIVNILRKYREDNNISKKEVLYYDTNIKQNEFVKNSLKSLSNSEISENKDTLFVDGNINVYIKESAEQKNTYIAKLKEKIANLESEVKRSNNILSNQNFIAKAPTSKVEEEKQKLAKYQEELDKYKEELK", "aa_hexdigest": "11acec314f31c8c1d10b522c436ddeb1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08264.19", "name": "Anticodon_1", "length": 78, "aa_cov": 0.3769633507853403, "hmm_cov": 0.9871794871794872, "evalue": 2.7369856393980292e-14, "score": 49.35884475708008, "start": 9, "stop": 80 }, { "id": "PF10458.14", "name": "Val_tRNA-synt_C", "length": 58, "aa_cov": 0.3036649214659686, "hmm_cov": 1.0, "evalue": 7.656459116935418e-13, "score": 44.672828674316406, "start": 134, "stop": 191 } ], "seq_stats": { "molecular_weight": 22322.269500000017, "isoelectric_point": 8.473611640930176 }, "id": "OAIKOHHJFB_480", "locus": "GCA000209735_00453" }, { "type": "cds", "sequence": "contig_1", "start": 246238, "stop": 246402, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGTATTTTTTTGGATTAGAATTCATGAAACAAAAACCTTTTGATAGAGTATTAATTCATGGCCTTGTTAGAGACCAATTTGGTAAAAAAATGTCTAAATCATCAAACAATGGTGTTGATCCAATTCAAGTTATAAATGAAAATGGTTCTGATGCTTTAAGATGA", "aa": "MYFFGLEFMKQKPFDRVLIHGLVRDQFGKKMSKSSNNGVDPIQVINENGSDALR", "aa_hexdigest": "91455ff6f6a1dbabfc0de21e00dfe881", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 54, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 2.4217413715810287e-18, "score": 62.12314224243164, "start": 1, "stop": 54 } ], "seq_stats": { "molecular_weight": 6204.080200000002, "isoelectric_point": 9.300805473327635 }, "id": "OAIKOHHJFB_481", "locus": "GCA000209735_00454" }, { "type": "cds", "sequence": "contig_1", "start": 246676, "stop": 246771, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACAAATTAGCTCAAGATATTTTAGACCACTTAAAATCAAAAGATGCAGTTAACTTTTATCCAAAAAGATTTGTTAATGTTATCAAAAAATGA", "aa": "MDKLAQDILDHLKSKDAVNFYPKRFVNVIKK", "aa_hexdigest": "76a673df5861ddef108e6deeff302e9d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3675.3042000000005, "isoelectric_point": 9.601679420471193 }, "id": "OAIKOHHJFB_482", "locus": "GCA000209735_00455" }, { "type": "cds", "sequence": "contig_1", "start": 246781, "stop": 247263, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF093D7" ], "nt": "ATGTACTATATCAAGTATCCAATCTTGAATTCTAATAAATATTTAACAGTTGCCACAGTTAGAACTGAAACTATGCTTAGTGACGTTGCTGTTATTTACAATCCAAAAGACAAAAGATATAAAGATTTTAAAGACATTCAAATTGTTCATCCTTTAACTAAACAAATAATTCCATTTGTGCCTGATGAATATGCAGACATGAAATTTGGTAGTGGCTTAATGAAATTATCAGCTCATGCTGAAGTTGATATTGACATTATCAAAAAACACAAATTACAAATTAATGAAACTATTGATAAAGACGGTTTAATTAACTGTCCAAAAAGTCAATTCCACAAATTAACTCGTCAAGAAGCAAGAGAAAAAATTGGTCAATATTTAGCTGATAATAATTTATTAGTTAAAGTAGAAACCATTACTTCAAATGTTGGTTATTCAGAACGTTCAAATACTCCAGTTGAAACTCTTGTTATGCCACAATGA", "aa": "MYYIKYPILNSNKYLTVATVRTETMLSDVAVIYNPKDKRYKDFKDIQIVHPLTKQIIPFVPDEYADMKFGSGLMKLSAHAEVDIDIIKKHKLQINETIDKDGLINCPKSQFHKLTRQEAREKIGQYLADNNLLVKVETITSNVGYSERSNTPVETLVMPQ", "aa_hexdigest": "4df4a5302e1b8becaf4a3335513dd746", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FF093D7", "query_cov": 0.84375, "subject_cov": 0.8709677419354839, "identity": 0.593, "score": 161.0, "evalue": 5.81e-48, "valid": false, "uniref50_id": "UniRef50_UPI002FF093D7" }, "pscc": { "uniref50_id": "UniRef50_UPI002FF093D7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF093D7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18421.091699999994, "isoelectric_point": 8.502171134948732 }, "id": "OAIKOHHJFB_483", "locus": "GCA000209735_00456" }, { "type": "cds", "sequence": "contig_1", "start": 247507, "stop": 247617, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGGATCATGCTGGTATCGCAACTCAAAGCAAAGTTGAAAATACAATTTATCAAAAAGAAGGATTAAACAGACATGATTTAGGAAGAGAGAAATTTCTTAAACGTGTTTGA", "aa": "MDHAGIATQSKVENTIYQKEGLNRHDLGREKFLKRV", "aa_hexdigest": "4580192047fb739f6a76c75b47c368e3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 35, "aa_cov": 0.9722222222222222, "hmm_cov": 1.0, "evalue": 1.1735318949777734e-07, "score": 26.926347732543945, "start": 1, "stop": 35 } ], "seq_stats": { "molecular_weight": 4183.7084, "isoelectric_point": 9.396476554870606 }, "id": "OAIKOHHJFB_484", "locus": "GCA000209735_00457" }, { "type": "cds", "sequence": "contig_1", "start": 248037, "stop": 248240, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATTTTCTGATTCATTGGTTGAATCACAAGAGTTTGTGAAAACAAATACAAAAAATAAAAAGCATTTAAGACGGAACTTAATATGCTTATTGAACTTAAGAAATAGACCATTTTTAAAGTTGGTTTATTTTTTAAAACAATTGTTTTATAGTGATTTTGAAAAATTACAGGGATTAAAAATGGATGATAAAAAGATCTAA", "aa": "MEFSDSLVESQEFVKTNTKNKKHLRRNLICLLNLRNRPFLKLVYFLKQLFYSDFEKLQGLKMDDKKI", "aa_hexdigest": "311a3de9d2fae3a25f6669830a300332", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8154.533599999995, "isoelectric_point": 9.721655082702636 }, "id": "OAIKOHHJFB_485", "locus": "GCA000209735_00458" }, { "type": "cds", "sequence": "contig_1", "start": 248227, "stop": 248370, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTCATCGTCAAGAGATTTCATTTGTTAAAATGTTTGAAAATATTTTACATATTAACGGAAGCAAAAATTATTATGTAGCTACATTGTTATATTTAATATTTAATTTTGTTTTAGGTTATGCAATTCATGGAATTTTCTGA", "aa": "MSHRQEISFVKMFENILHINGSKNYYVATLLYLIFNFVLGYAIHGIF", "aa_hexdigest": "5c39bea7bddd000749e6b6aa04520755", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5553.4569999999985, "isoelectric_point": 8.157845497131351 }, "id": "OAIKOHHJFB_486", "locus": "GCA000209735_00459" }, { "type": "cds", "sequence": "contig_1", "start": 248905, "stop": 249096, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGACACAAATTCAATATTTTTTGTATAATTTTAAGCATGGATACAATGTCAAAATTTTATTTAAAATTTAATACTGAATTTGCAAATGGAAGACCAGAAGCTTTTAGCGCATTCCACTTGACATTTTTAGCAACATTTCTTATTTTAGGCATCATTTTATGTGTTGCTTTAAGAAATAGAAAAGAATGA", "aa": "MGHKFNIFCIILSMDTMSKFYLKFNTEFANGRPEAFSAFHLTFLATFLILGIILCVALRNRKE", "aa_hexdigest": "73c5e07f6cf408f0ac09f2c2f8fe65fa", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7292.634699999997, "isoelectric_point": 9.236852836608886 }, "id": "OAIKOHHJFB_487", "locus": "GCA000209735_00460" }, { "type": "cds", "sequence": "contig_1", "start": 249174, "stop": 249482, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGAAGCAAAGAAAAAAACAACAGCTGCAAATAAAGCTAAAAAACCAGTAGCAACCAAAACAACAAAAAAAGTTACAGCAGCTAAAACAATTAAAAAACCAGCTGCAACAAAAGCAAAAGTAGCTAAAAAACCAACATTAGATAAATATTATATCTCAGAAAAACCAATCAAAAAAACTGGTGATATTGAATTCAACTTTAAAAAAGCAAAACAAAAGACATCAAGAAAATATGATGAATTTATCGATGCTCTTAAAGAATTTATTTTTGTTAGCGAAACATCGAAAAATGATACAAGAGTTTGA", "aa": "MAEAKKKTTAANKAKKPVATKTTKKVTAAKTIKKPAATKAKVAKKPTLDKYYISEKPIKKTGDIEFNFKKAKQKTSRKYDEFIDALKEFIFVSETSKNDTRV", "aa_hexdigest": "19970ec1eb8ce9ec7cfbfc635afd95e5", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11472.35979999999, "isoelectric_point": 10.115041160583491 }, "id": "OAIKOHHJFB_488", "locus": "GCA000209735_00461" }, { "type": "cds", "sequence": "contig_1", "start": 249729, "stop": 250079, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGCTTCAGAAGTGCAAAAAGATGACATTGTTGCTTCAACACCAACAAGAGGTTGTGAAGTTTTTGTAACAAACATTTATCCAAGCAATGGACAAATGGAAGTTGAATATGTTGTTAAAAATGAAAACAATCAATCAGATTCTCAAATCAAAACTTTATATGGATTTAAACAAAATCCAGTTACTGAAGAAATAATGACTATTTCTGATGAAGAAGAAAAAAAATTAGAATCTAATATTGTTGAAGACACAGAACCTGCTGAAGTTGTTGATGTAGAAAGTGATGAAAATAAAAATACATCAGTTGAAAATGACAAAAAGTCAAAAATGTCAAAAGAACTTTTATGA", "aa": "MLASEVQKDDIVASTPTRGCEVFVTNIYPSNGQMEVEYVVKNENNQSDSQIKTLYGFKQNPVTEEIMTISDEEEKKLESNIVEDTEPAEVVDVESDENKNTSVENDKKSKMSKELL", "aa_hexdigest": "fcad94414cc4237ce97bcef126f916cd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13095.280099999996, "isoelectric_point": 4.27033634185791 }, "id": "OAIKOHHJFB_489", "locus": "GCA000209735_00462" }, { "type": "cds", "sequence": "contig_1", "start": 250304, "stop": 250645, "strand": "-", "frame": 1, "gene": null, "product": "Integral membrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XED9", "UniRef:UniRef90_C4XED9" ], "nt": "ATGATATTTATTCCAAATGAAGTCTTAAATAAATCGATATTTTTTGATTGTCACTCAATGATTCATCATGGAATTTTAATGTTAATTGGTATTGTTTTAATTTTTAATAATTATTCAAAATTCGACAAAGGAAATTATATTATTCATAATCTTTTTATGTTCCTAATAATGTTTAGTGTAGTAGTAATATTGAATGAAATTTTTTATCAAACAGCACACAAAGACTTAAATAAATTACAAGAAAATTATCCAAATTTATTAGCAATTAGTCATCATCTTAATAATCATTTGACACTATTATTTGAGAAAATTTTTAGTGTTAAATTAAATGGCAACTACTGA", "aa": "MIFIPNEVLNKSIFFDCHSMIHHGILMLIGIVLIFNNYSKFDKGNYIIHNLFMFLIMFSVVVILNEIFYQTAHKDLNKLQENYPNLLAISHHLNNHLTLLFEKIFSVKLNGNY", "aa_hexdigest": "59a1d99c492e5aadd43b78defb7acdf7", "start_type": "ATG", "rbs_motif": "GGxGG", "genes": [], "truncated": "both", "pseudogene": { "start": 250181, "stop": 250819, "observations": { "mutation": [ 250306, 250783, 250801 ], "stop": [ 250306, 250783, 250801 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XED9", "query_cov": 1.0, "subject_cov": 0.5330188679245284, "identity": 1.0, "score": 225.0, "evalue": 2.27e-73, "gene_start": 1, "gene_end": 113, "reference_start": 59, "reference_end": 171, "reference_sequence": "MDKDNVWYSKYKWSYFPLFFCSIPYFLFPIYLINQNKNLNKILIDFVSFTSLYGGISIMIFIPNEVLNKSIFFDCHSMIHHGILMLIGIVLIFNNYSKFDKGNYIIHNLFMFLIMFSVVVILNEIFYQTAHKDLNKLQENYPNLLAISHHLNNHLTLLFEKIFSVKLNGNYWVITLLYPVINFVLALAVYSLIIIIKVSNKIPENKNKMSFQ" }, "paralog": false, "identity": 0.9811320754716981, "score": 416.0, "evalue": 8.43e-151, "description": "Internal stop codon at 250306, 250783, 250801. Nonsense mutation around 250306, 250783, 250801.", "psc": { "uniref90_id": "UniRef90_C4XED9", "product": "Integral membrane protein", "uniref50_id": "UniRef50_C4XED9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XED9", "UniRef:UniRef50_C4XED9" ] }, "pscc": { "uniref50_id": "UniRef50_C4XED9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XED9" ], "product": "Integral membrane protein" } }, "id": "OAIKOHHJFB_490", "locus": "GCA000209735_00463" }, { "type": "cds", "sequence": "contig_1", "start": 251108, "stop": 251383, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGGCCATAAAAATGAAAATAATTCTTTATTAGTAACTGATGAATTGAGTCAAGAAAAAACTATTGAAACACAAATAAATGATTTAGAGAATCCTTTATTAGAAAAGACAATTATGATAGCTGCTTTCCAGCCTAATAAAATTAAAAATAACAAAAATTTGGATAAAACATTTACTCAAGAATTAGAAGCTATTCAAAAAGTAAATAATAGTAATCAAAAAGTTGAAACTGACTTGACAATGACTCAAGTATTATCAGATGACATATATTAA", "aa": "MQGHKNENNSLLVTDELSQEKTIETQINDLENPLLEKTIMIAAFQPNKIKNNKNLDKTFTQELEAIQKVNNSNQKVETDLTMTQVLSDDIY", "aa_hexdigest": "f1d9a50958cafc651587f3115446cffd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10449.592199999994, "isoelectric_point": 4.6114839553833 }, "id": "OAIKOHHJFB_491", "locus": "GCA000209735_00464" }, { "type": "cds", "sequence": "contig_1", "start": 251384, "stop": 251977, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGACTTTGTATTTAATAATGTTATTGTTCTTAAAAATAGTTTAGTTTTAGCTAAAAAAGTTTCTAATAAAATTCTTCACAAATTAGACCAATTTGACAGATTAATTGAAATTTTTGAAAAATCATCAATCAATACAAAGAGCTTAATTAGACCTTATGAAAATATTTCAAAAATTAACAAACTAAAAATGAACTTTATTTCTTCTATTATTAAGAAACCAAAACTAATTATTATTGCTGATGATCCTGATATTAAAGATACAAAACTTAAATTTGAATTTTTAAGAGCTGCTTTTGAATTATGTGAACAAAGTAAAATAAATATTATTTTTGTTACTCAAGATAAAGAATTAGTTGAAGACAATGAGTTTGACTATATCTATTTGATTGCTAATAATAAAGAAGTTGAATTTGGAGATTTAAAAGAAGTTTTAAATAACACAATTAACCCTGAAATTAAAAGTAAAATTAATGACCAAGCCATTTCACAAGAAATTATTAGAAGTCAAAAAGAATTTGTTTTTAGTGATATTTATGATGTAGATGAAGAGCACTACATTATTACACCTTCAAAATATTTCAAATTATGA", "aa": "MFDFVFNNVIVLKNSLVLAKKVSNKILHKLDQFDRLIEIFEKSSINTKSLIRPYENISKINKLKMNFISSIIKKPKLIIIADDPDIKDTKLKFEFLRAAFELCEQSKINIIFVTQDKELVEDNEFDYIYLIANNKEVEFGDLKEVLNNTINPEIKSKINDQAISQEIIRSQKEFVFSDIYDVDEEHYIITPSKYFKL", "aa_hexdigest": "459813f0047f9350c5f0921f759b0fd1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23178.578100000006, "isoelectric_point": 5.581043434143066 }, "id": "OAIKOHHJFB_492", "locus": "GCA000209735_00465" }, { "type": "cds", "sequence": "contig_1", "start": 252209, "stop": 253291, "strand": "-", "frame": 1, "gene": null, "product": "MAG1360 family OppF-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LI97", "UniRef:UniRef90_C4XEE0" ], "nt": "ATGGCTGAAAAAGAAGAACAAAATTTACAATATCCTTTAGTTTACAAATATGAAGAACTTAAAAAAAATTACGGTGAAAACAAGGAACGTATTAACCAATTAAATAAGACAGTTAATAAATACTATTATCCTGTTCGTCATGTTATTTTGAATATTTTAGTAGAACAAGCTAACTCATTAATGGCGATTTTTCGTGATTTTGAAAGAAACTTCAATAGTTTTTTAAATAAAACAACAAAAAATATTGCTGAATATAACTTTGATGAATTACAAACAAGATTTAAAGAAATTGAAAGCTACCAAGACTTATTTTTTGGTGAACAAATTACTTTAATATTAGACTTGTTTTCTCAAGTATTTAATAAATTTGAATTCTTTTATAATGCATCTAAATCAAATATTGACTACTTGGGTGATGAACAAATAAATCACTTAAAAAAGAGACTTTACTACTTAACCAAAATGAGAAAAACTTCTAAAAAAAGAGTGGAAGACAACTTAAAAATTAGAGACTTAAAACATGATATTTGTGCTTTAGATGAAATAGCAAAAGAAATTTGTCACAACTCAGATCAAAAAATTAAATACTTTGCTTTTAGATATAAAAGAAGTGCCGAAATTGATTTGTATAAATCAACTTTTTTTGCTAAAGAAAGCTCACAAAGACAAATTTTAAACAAAAGATATTTTGTTAAAATGTTTGCTCACAAGTTACTTAAAAAATATAAAAAATATTTTAAATATTTAAAAATTGATGAACTTGATGAAATGAGAAGAACACTAGATACCACCATCAAATTATTTATTAGCAATAGTTTAGGAAATTTAACTTACAGCAATAAAAACTTTTCAATTAAAAAAATTAAAAAGATTCTTAAAGAAGAATTCTACTTTAATGTCGAAAGTTATAAAATCAAATCAAAAAGCAATTTTGACTTAATTAAAGATTCAAAAGATCAAATTCAAAGTCAAATAATGTCTATTAAAAATAGATCATATGGAACATACTTCAATACTGTCCCTGAAAGATCTATCAAAGTTGCAAAAGAAATTTTAACTAATGCTCAAAGTGAACATAATTGA", "aa": "MAEKEEQNLQYPLVYKYEELKKNYGENKERINQLNKTVNKYYYPVRHVILNILVEQANSLMAIFRDFERNFNSFLNKTTKNIAEYNFDELQTRFKEIESYQDLFFGEQITLILDLFSQVFNKFEFFYNASKSNIDYLGDEQINHLKKRLYYLTKMRKTSKKRVEDNLKIRDLKHDICALDEIAKEICHNSDQKIKYFAFRYKRSAEIDLYKSTFFAKESSQRQILNKRYFVKMFAHKLLKKYKKYFKYLKIDELDEMRRTLDTTIKLFISNSLGNLTYSNKNFSIKKIKKILKEEFYFNVESYKIKSKSNFDLIKDSKDQIQSQIMSIKNRSYGTYFNTVPERSIKVAKEILTNAQSEHN", "aa_hexdigest": "60d9e0ab51f4738d19f5480ecea7d40e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 251906, "stop": 253591, "observations": { "mutation": [ 252076, 252211, 253348 ], "stop": [ 252076, 252211, 253348 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEE0", "query_cov": 1.0, "subject_cov": 0.43373493975903615, "identity": 1.0, "score": 659.0, "evalue": 6.07e-231, "gene_start": 1, "gene_end": 360, "reference_start": 104, "reference_end": 463, "reference_sequence": "MLFINMKKNKIFGIENCFKNLNFEDDYVDLQKIEIYDQLKTAILVTDKNRNLIGSNFYNTIFNNNLNILSFFSKNNKKGSGTNYWNIEFNKNNNAFSEINFFNMAEKEEQNLQYPLVYKYEELKKNYGENKERINQLNKTVNKYYYPVRHVILNILVEQANSLMAIFRDFERNFNSFLNKTTKNIAEYNFDELQTRFKEIESYQDLFFGEQITLILDLFSQVFNKFEFFYNASKSNIDYLGDEQINHLKKRLYYLTKMRKTSKKRVEDNLKIRDLKHDICALDEIAKEICHNSDQKIKYFAFRYKRSAEIDLYKSTFFAKESSQRQILNKRYFVKMFAHKLLKKYKKYFKYLKIDELDEMRRTLDTTIKLFISNSLGNLTYSNKNFSIKKIKKILKEEFYFNVESYKIKSKSNFDLIKDSKDQIQSQIMSIKNRSYGTYFNTVPERSIKVAKEILTNAQSEHNWKFNKIQSEFYNTLKSNQKLLNSYDTMTLINSKIKRKIMKLKTSLWFKRIFNRSINEKDKLNIKNFSANLKKIIEKFEMFDFVFNNVIVLKNSLVLAKKVSNKILHKLDQFDRLIEIFEKSSINTKSLIRPYENISKINKLKMNFISSIIKKPKLIIIADDPDIKDTKLKFEFLRAAFELCEQSKINIIFVTQDKELVEDNEFDYIYLIANNKEVEFGDLKEVLNNTINPEIKSKINDQAISQEIIRSQKEFVFSDIYDVDEEHYIITPSKYFKLWMQGHKNENNSLLVTDELSQEKTIETQINDLENPLLEKTIMIAAFQPNKIKNNKNLDKTFTQELEAIQKVNNSNQKVETDLTMTQVLSDDIY" }, "paralog": false, "identity": 0.9875222816399287, "score": 1049.0, "evalue": 0.0, "description": "Internal stop codon at 252076, 252211, 253348. Nonsense mutation around 252076, 252211, 253348.", "psc": { "uniref90_id": "UniRef90_C4XEE0", "product": "MAG1360 family OppF-related protein", "uniref50_id": "UniRef50_A0A3P8LI97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEE0", "UniRef:UniRef50_A0A3P8LI97" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LI97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LI97" ], "product": "ABC transporter ATP-binding protein" } }, "id": "OAIKOHHJFB_493", "locus": "GCA000209735_00466" }, { "type": "cds", "sequence": "contig_1", "start": 253346, "stop": 253585, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAAATAAAATATTTGGAATTGAAAATTGTTTTAAGAATCTAAATTTTGAAGATGATTATGTAGATTTACAAAAAATTGAGATTTACGATCAACTTAAAACTGCAATTTTAGTTACTGATAAAAACAGAAACCTAATTGGTTCCAATTTTTACAATACAATTTTCAATAACAATTTGAACATTTTATCTTTCTTTTCTAAGAATAATAAAAAAGGTTCAGGAACAAATTATTGA", "aa": "MKKNKIFGIENCFKNLNFEDDYVDLQKIEIYDQLKTAILVTDKNRNLIGSNFYNTIFNNNLNILSFFSKNNKKGSGTNY", "aa_hexdigest": "7fde6c4fb76b82243f2d789bc966aacc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9251.382599999994, "isoelectric_point": 9.068719291687014 }, "id": "OAIKOHHJFB_494", "locus": "GCA000209735_00467" }, { "type": "tmRNA", "sequence": "contig_1", "start": 253652, "stop": 254029, "strand": "+", "gene": "ssrA", "product": "transfer-messenger RNA, SsrA", "db_xrefs": [ "SO:0000584" ], "tag": { "start": 253735, "stop": 253824, "aa": "AEDKKAEEVNISSLMIAQKMQSQSNLAFA", "nt": "GCAGAAGATAAAAAAGCAGAAGAAGTTAATATTTCTTCACTTATGATTGCACAAAAAATGCAATCACAATCAAACCTTGCTTTCGCTTAG" }, "nt": "GGGGGTGTAATGGCTTCGACATGCATTGGGTGATACTAATATCAGTAGTTTGGCAGACTATAATGCATCTAGGCTTTATAATCGCAGAAGATAAAAAAGCAGAAGAAGTTAATATTTCTTCACTTATGATTGCACAAAAAATGCAATCACAATCAAACCTTGCTTTCGCTTAGTTAAAAGTGACAAGTGGTTTTAAAGTTGACATTTTCCTATATATTTTAAAATCGGCTTTTAAGGAGAACAGGAGTCTGAAAGGGTTCCAAAAATCTATATTGTTTGCATTTCGGTAGTATAGATTAATTAGAAATGATAAACTGTAAAAAGTATTGGTATTGACTTGGTGTGTGGACTCGGGTTCAACTCCCGACATCTCCACCA", "id": "OAIKOHHJFB_37", "locus": "GCA000209735_00468" }, { "type": "cds", "sequence": "contig_1", "start": 254158, "stop": 254259, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGGAATATCAGAAGAATTATTAAAAGGCAAAAGTAATGTTATGTCTTTAGGCAAATTGAACAATATAATTAAAGACAATTTAAAAAACAACTTTTGA", "aa": "MEGISEELLKGKSNVMSLGKLNNIIKDNLKNNF", "aa_hexdigest": "6d44cb23e3ab1d5550544ec4465c6797", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3692.2652000000007, "isoelectric_point": 8.140632438659669 }, "id": "OAIKOHHJFB_495", "locus": "GCA000209735_00469" }, { "type": "cds", "sequence": "contig_1", "start": 254434, "stop": 255228, "strand": "+", "frame": 1, "gene": "xseA", "product": "exodeoxyribonuclease VII large subunit", "db_xrefs": [ "EC:3.1.11.6", "GO:0006308", "GO:0008855", "GO:0009318", "KEGG:K03601", "SO:0001217", "UniRef:UniRef50_C4XEE1", "UniRef:UniRef90_C4XEE1" ], "nt": "ATGAATGTTAGCATATCGCCATATTCTTTAAATAATTCAGTTTCTTATTCAATTGAACAAATTATTGATTTAGATACTTATTCAAAAGAGAAAATTGAAAAAGAAAAATTAAAGAAACTTTATTTATCAAAAGGTTATTTTGATCCTAATAGAAAGAGACCAATTCCAAAATACTGTGAATATATTGCGGTTATTGCTGCAGAAAGTGGTCAAGCAGTTCGAGATGTTTATACAACTGTTTTATCAAGATTTCCAATTGCAAATTTAAAATTATTTAATTCATCAGTTCAAGGCCAACAAGCCTTAATTGATATACCGAAGCAAATTGCAAATGCTAATAAAGAAAACTTTGATGTTATTATTATTGCTCGTGGTGGTGGAAGTACAGTTGATTTAGAAATCTTTAACCAAGAAGCAATTGTTAATGCTATTGTTGAAAGTAAAATTCCAATTGTTACAGCTATTGGTCATACAGATGATATTAGTTTTAGTGATTTAATAGCAGATGCTTATGGTAATACTCCAACAGGAGCAGCAAAATTAGTTGTGCCTGATAAGGAAGAAATTCTAAGTTTATTAAGAAACTATCGAGCAAGAAATCAAAATTATATTAACAAAATGCTTTTGGATAGTCAAAATAAATTAAAAAAATTGGCTCAAAAAATTTATGAATTTAAACCAGAGTTAATAATTGAACAATATGAAAATAAAGTTAATAAATTTAAAGAATTTATAAAAAATCATAAAATTCTTTTATTACAAAAGTTAGAAACTAAAATTAAAGAATTTAAATAA", "aa": "MNVSISPYSLNNSVSYSIEQIIDLDTYSKEKIEKEKLKKLYLSKGYFDPNRKRPIPKYCEYIAVIAAESGQAVRDVYTTVLSRFPIANLKLFNSSVQGQQALIDIPKQIANANKENFDVIIIARGGGSTVDLEIFNQEAIVNAIVESKIPIVTAIGHTDDISFSDLIADAYGNTPTGAAKLVVPDKEEILSLLRNYRARNQNYINKMLLDSQNKLKKLAQKIYEFKPELIIEQYENKVNKFKEFIKNHKILLLQKLETKIKEFK", "aa_hexdigest": "9f3fcd30e93deb297757f71784593c89", "start_type": "ATG", "rbs_motif": null, "genes": [ "xseA" ], "truncated": "5-prime", "pseudogene": { "start": 254158, "stop": 255531, "observations": { "mutation": [ 254257, 254269, 254359 ], "stop": [ 254257, 254269, 254359 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEE1", "query_cov": 0.9545454545454546, "subject_cov": 0.5502183406113537, "identity": 1.0, "score": 478.0, "evalue": 5.29e-167, "gene_start": 1, "gene_end": 252, "reference_start": 93, "reference_end": 344, "reference_sequence": "MEGISEELLKGKSNVMSLGKLNNIIKDNLKNNFWQTYWIKASISKLSIKNHAYLNIVDGEYSSNAAIWASKIPNIGIDITSNNKDLYSHAYFMNVSISPYSLNNSVSYSIEQIIDLDTYSKEKIEKEKLKKLYLSKGYFDPNRKRPIPKYCEYIAVIAAESGQAVRDVYTTVLSRFPIANLKLFNSSVQGQQALIDIPKQIANANKENFDVIIIARGGGSTVDLEIFNQEAIVNAIVESKIPIVTAIGHTDDISFSDLIADAYGNTPTGAAKLVVPDKEEILSLLRNYRARNQNYINKMLLDSQNKLKKLAQKIYEFKPELIIEQYENKVNKFKEFIKNHKILLLQKLETKIKEFKELVKSIKELFLQKIQAKFEMAKTRFKNQALHCFNNQLATFSIVKEKVIQRNPLKILEKGFAKVTNENNQIIKTIKQVKVDKKLYIELKDGIAEVVVQKVNKK" }, "paralog": false, "identity": 0.9912472647702407, "score": 806.0, "evalue": 1.04e-298, "description": "Internal stop codon at 254257, 254269, 254359. Nonsense mutation around 254257, 254269, 254359.", "psc": { "uniref90_id": "UniRef90_C4XEE1", "gene": "xseA", "product": "exodeoxyribonuclease VII large subunit", "ec_ids": [ "3.1.11.6" ], "uniref50_id": "UniRef50_C4XEE1", "kegg_orthology_id": "K03601", "go_ids": [ "GO:0006308", "GO:0008855", "GO:0009318" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEE1", "EC:3.1.11.6", "UniRef:UniRef50_C4XEE1", "KEGG:K03601", "GO:0006308", "GO:0008855", "GO:0009318" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEE1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEE1" ], "product": "Exodeoxyribonuclease VII large subunit" } }, "id": "OAIKOHHJFB_496", "locus": "GCA000209735_00470" }, { "type": "cds", "sequence": "contig_1", "start": 255283, "stop": 255534, "strand": "+", "frame": 1, "gene": null, "product": "Exonuclease VII large subunit C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEE2", "UniRef:UniRef90_C4XEE2" ], "nt": "ATGGCTAAAACGAGATTCAAGAATCAAGCCTTGCATTGTTTCAATAATCAATTAGCAACTTTTTCAATAGTAAAAGAAAAAGTTATTCAACGTAATCCATTAAAAATATTAGAAAAAGGTTTTGCAAAAGTTACAAATGAAAACAACCAAATAATTAAAACAATAAAACAAGTTAAAGTTGATAAAAAACTTTACATTGAATTAAAAGATGGTATAGCTGAAGTAGTTGTACAAAAAGTTAATAAAAAATAA", "aa": "MAKTRFKNQALHCFNNQLATFSIVKEKVIQRNPLKILEKGFAKVTNENNQIIKTIKQVKVDKKLYIELKDGIAEVVVQKVNKK", "aa_hexdigest": "6faac461e12bae5fa60c055331936e23", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XEE2", "query_cov": 1.0, "subject_cov": 0.8217821782178217, "identity": 1.0, "score": 158.0, "evalue": 8.47e-49, "valid": true, "product": "Exonuclease VII large subunit C-terminal domain-containing protein", "uniref50_id": "UniRef50_C4XEE2" }, "pscc": { "uniref50_id": "UniRef50_C4XEE2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEE2" ], "product": "Exonuclease VII large subunit C-terminal domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_497", "locus": "GCA000209735_00471" }, { "type": "cds", "sequence": "contig_1", "start": 255544, "stop": 255732, "strand": "+", "frame": 1, "gene": "xseB", "product": "exodeoxyribonuclease VII small subunit", "db_xrefs": [ "EC:3.1.11.6", "GO:0006308", "GO:0008855", "GO:0009318", "RefSeq:WP_013354436.1", "SO:0001217", "UniParc:UPI0001E32FD5", "UniRef:UniRef100_C4XEE3", "UniRef:UniRef50_C4XEE3", "UniRef:UniRef90_C4XEE3" ], "nt": "ATGTCAGAAAAATTAAAATTTAATCAAATCATGAATCAAATAGATGAAATTAGCAACAAACTCGGATCAAACATTGATTTAGAGGATTCCATTAATTTATACAAACAAGCTGAAAAATTAATAGAAGAAGCTGAAACAAAACTAGTTGAAGTTAGAAAAATCGTTGCTAAAGAATCTATAAAACAATAA", "aa": "MSEKLKFNQIMNQIDEISNKLGSNIDLEDSINLYKQAEKLIEEAETKLVEVRKIVAKESIKQ", "aa_hexdigest": "bb796ea96a719634f36991ffb4c5135f", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32FD5", "ncbi_nrp_id": "WP_013354436.1", "uniref100_id": "UniRef100_C4XEE3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FD5", "RefSeq:WP_013354436.1", "UniRef:UniRef100_C4XEE3" ] }, "ips": { "uniref100_id": "UniRef100_C4XEE3", "uniref90_id": "UniRef90_C4XEE3" }, "psc": { "uniref90_id": "UniRef90_C4XEE3", "gene": "xseB", "product": "exodeoxyribonuclease VII small subunit", "ec_ids": [ "3.1.11.6" ], "uniref50_id": "UniRef50_C4XEE3", "go_ids": [ "GO:0006308", "GO:0008855", "GO:0009318" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEE3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEE3" ] }, "genes": [ "xseB" ], "id": "OAIKOHHJFB_498", "locus": "GCA000209735_00472" }, { "type": "cds", "sequence": "contig_1", "start": 255907, "stop": 256206, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACAAATTTTTGCAAACGATACAAACTCTATTAAAAGAATTGATGTTCAAAAAATGTATGCATCAAAACATAAAAAATTAGTTAATAACCTTTTAGATATTAAAAGTAAAGGCGATAATGCGACATTTTTTAAGACTTCAAATTTATCTTTAGATCTTAAAAAACTTATTGGAATTAAAAACTTAAGAGAAATTTTAAACAATGTTGAATTTAAAATTGCACTCAAAAATTCTTTTGATTCTAAAAAACTAAATGAATTAAAAAACATTAATACAGTTAGTCAATTTGAAGTCTGA", "aa": "MKQIFANDTNSIKRIDVQKMYASKHKKLVNNLLDIKSKGDNATFFKTSNLSLDLKKLIGIKNLREILNNVEFKIALKNSFDSKKLNELKNINTVSQFEV", "aa_hexdigest": "2eef6b667d819f30d1c02bf12a9934cd", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11401.177699999987, "isoelectric_point": 9.901650810241698 }, "id": "OAIKOHHJFB_499", "locus": "GCA000209735_00473" }, { "type": "cds", "sequence": "contig_1", "start": 256603, "stop": 257553, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12" ], "nt": "TTGACTGAAGCATTAAACAGTATTAAAAATATAGACAGTAAATATTTGAACAATTTAGATTATAAAGAAACTATATTCCAAGAACCATTTGCTGAATATTCTAAAGTCCAATTAGAAAATAATGGTTGTGTCGAACTATTTCCCGGAACTGTATTAATTGAAGCAAATCCAGGCGGTACTAAATTGCGTGAAGCAATGATTAATTTAATTTTAAATAACAAAATAGACAACTTGATTGAAATAGATGACTTAAAAGATTATACTAAAACAGCTTATGAAAAAGCATTAGGCAAAGGCAATATTGCACGTGTAACACCATCAGATTTTAGTCAAGAAAAAGCAATTATAGGAACACTAAATAATTCAGCAATTATTATTGGTCCTCCTGGTACAGGAAAGAGTCAAACTATTGCAAATATCTTAGTTAACATTGCGAAAGAAAACAAAAAAGCTTTATTTATTTCTCAAAAGAAAGTAGCTTTAGATGTTGTTTTAAAACGTCTTGGAAAATATCAAAATATGATGTTTCAATTCTCTGAAACAGCTAAAGTAAATCAAAGTGAAAAAGAATACTTTTATAAACCAATTATTGAGTATTTTAAAGAAATTAATGAAGTCCAAGAAAACCATGATATTTCTAAATATAATTCAAGCTTTTTCTCTAAAGGAGAGTTCAAATATTTAGATGCAAAAAGTGAAATTGGTGAAATATTTTCAAAAGACTTAAACTGTTATTTTAACCTAAAAAATAAGGGAAAAATTGAACAAAATATACCAAAATTTATTCAATTTTATAAGTTATATAATGAACTATTTTCTAAGAATAAATTTTTAAAAATATTCAACAATTATAAAACACTTTCAAAACGCGAAATAGCCTATAAAACAGGTTATTTGAAAAAATATGCAACTATTTTTACTAAATTACCAGCAGAGTTTAAAAGACTATGA", "aa": "MTEALNSIKNIDSKYLNNLDYKETIFQEPFAEYSKVQLENNGCVELFPGTVLIEANPGGTKLREAMINLILNNKIDNLIEIDDLKDYTKTAYEKALGKGNIARVTPSDFSQEKAIIGTLNNSAIIIGPPGTGKSQTIANILVNIAKENKKALFISQKKVALDVVLKRLGKYQNMMFQFSETAKVNQSEKEYFYKPIIEYFKEINEVQENHDISKYNSSFFSKGEFKYLDAKSEIGEIFSKDLNCYFNLKNKGKIEQNIPKFIQFYKLYNELFSKNKFLKIFNNYKTLSKREIAYKTGYLKKYATIFTKLPAEFKRL", "aa_hexdigest": "1210eea2f1b59b6bc06462f9fd20d031", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 74, "aa_cov": 0.20253164556962025, "hmm_cov": 1.0, "evalue": 4.763707991897561e-10, "score": 35.706966400146484, "start": 109, "stop": 172 } ], "seq_stats": { "molecular_weight": 36422.52059999997, "isoelectric_point": 9.223701286315915 }, "id": "OAIKOHHJFB_500", "locus": "GCA000209735_00474" }, { "type": "cds", "sequence": "contig_1", "start": 257626, "stop": 258093, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12" ], "nt": "ATGTCAAACTTTATTAGACTTAATTCATTTATTGAATTTGATAAAAATGAAAAGGATTACAACAAATTTTTAGAAACTAAAAAGTCAGATAATACCACACCTGAACTATTAGAAATGATTTATGAAAATAATGCTTTTGAAGCTAAAAAACGTTTAGATGACTATCGTCAAAAAAGTGAAGCACAACGTCAAAAATTAAACAAATTTTTTGGACGTTTAGAACGTAAAATTACACCTCCTTATATTATTAGTTCATTGTTTAAAGATATCATTAAAAATGTATACAACATTTATGTTGGTACACCTGAAATTTTAGGTAACTTTATTGATTTTGAAAATGACCATTATGATTATGTAATCTTCGATGAGGCTAGTCAGATTTACATTGAAAAAGCCATTCCTTTTATTTCAATAGCTGACAAAGTTATTGTTGCTGGCGATAATCAACAAATGCAGCCAAGTAACTGA", "aa": "MSNFIRLNSFIEFDKNEKDYNKFLETKKSDNTTPELLEMIYENNAFEAKKRLDDYRQKSEAQRQKLNKFFGRLERKITPPYIISSLFKDIIKNVYNIYVGTPEILGNFIDFENDHYDYVIFDEASQIYIEKAIPFISIADKVIVAGDNQQMQPSN", "aa_hexdigest": "945dd55853591f3ee62037c74490ae5b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 139, "aa_cov": 0.8903225806451613, "hmm_cov": 0.8920863309352518, "evalue": 5.640524982917057e-10, "score": 35.46651840209961, "start": 16, "stop": 153 } ], "seq_stats": { "molecular_weight": 18345.52439999998, "isoelectric_point": 5.138608741760252 }, "id": "OAIKOHHJFB_501", "locus": "GCA000209735_00475" }, { "type": "cds", "sequence": "contig_1", "start": 258175, "stop": 258333, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTAAATATTTACTTGAAATGAATTACCGTTCAAATGCTAGTGAGCTTGTTTTATTTTCTTCAAAAGAATTCTATGAATCAGGCCTTAAAGGTTTGGATAACTATCCAAGAAAAGAAAAAACTAGTGTTGAAGTTATTGATGTTGATGGTCAATGA", "aa": "MPKYLLEMNYRSNASELVLFSSKEFYESGLKGLDNYPRKEKTSVEVIDVDGQ", "aa_hexdigest": "939073b8192658e69ba8e270e9ded91f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6019.720900000002, "isoelectric_point": 4.893632698059082 }, "id": "OAIKOHHJFB_502", "locus": "GCA000209735_00476" }, { "type": "cds", "sequence": "contig_1", "start": 258367, "stop": 258738, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13087.12" ], "nt": "ATGATCCAAGTTTGTGAAAAGAATATCAAAAAATACAACTCAATTATTCTTCTAGCATTCAATAGAAAACAACAAGATTACATTCGTGAACAAATTGCCTTAAATAGTCCAAAAATCTTTGCTGAATTAGAAGACAAAGTTATCTTAAGAAACATTGAAAACATCCAAGGCGATGAAGCAGAATTAGTTATTGCTTCAGTAGGTTATACTGGTAAAACTGCTTTACATTCAACTTACATTGGTTCTAAAAAAGGTCGAAATGCTTTAAATGTTGCTATTACTAGAGCTAAAGATAAAATGATAATTATTAAAAGTATCAAAACTAGTGAAGTTGATGCTAAGAGTGAAAACTTGATGACATTTAAACGTTGA", "aa": "MIQVCEKNIKKYNSIILLAFNRKQQDYIREQIALNSPKIFAELEDKVILRNIENIQGDEAELVIASVGYTGKTALHSTYIGSKKGRNALNVAITRAKDKMIIIKSIKTSEVDAKSENLMTFKR", "aa_hexdigest": "224945089dfb186540a8e58bbdf3938f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13087.12", "name": "AAA_12", "length": 97, "aa_cov": 0.7886178861788617, "hmm_cov": 0.8969072164948454, "evalue": 2.4389440247509257e-12, "score": 42.88240432739258, "start": 7, "stop": 103 } ], "seq_stats": { "molecular_weight": 13944.106899999977, "isoelectric_point": 9.575053977966306 }, "id": "OAIKOHHJFB_503", "locus": "GCA000209735_00477" }, { "type": "cds", "sequence": "contig_1", "start": 258928, "stop": 259134, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATAATAAAAGCCAATTTGTAGTTGGCTTAAATGTAGATAGTTCAAGTGATGACATTGAAATTGATGATTTCATTGAAAAAAGAATAAAAACTGATTTTATGTCAGTTAAAGGATACCCAATTTATCGTGTTTCTAACTTTAGATTTGCAGAAAATCAGCCTTCAATTTATCGCTTTTTAAACACTTTAATTAGTAAAGCTTAA", "aa": "MDNKSQFVVGLNVDSSSDDIEIDDFIEKRIKTDFMSVKGYPIYRVSNFRFAENQPSIYRFLNTLISKA", "aa_hexdigest": "6cac69579f4fd64bfaa62e3c79a728c0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7914.845199999997, "isoelectric_point": 5.161571693420409 }, "id": "OAIKOHHJFB_504", "locus": "GCA000209735_00478" }, { "type": "cds", "sequence": "contig_1", "start": 259541, "stop": 259909, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTGCTGCAGCAACTGCAATTACATTAGGTTCACTTTATGGATGTGCGAAAAATTCAGATGAAAAACTTGGCAAGGCACATACGGGTAATCCTAAAGACTTTAAGAATTATTTTGTTAGTGTTGATAAAGCTGAAAGTAATTTTGTGACAACAGACAAAAGTAAAAAAGTTGCAAAATTTGACTTTGCTAAAAATCAAGTTATTAAAGAAAATGGTCAAGCTATTAGTGTTGACGAATTTTTAGATCAATATTATCGTGAACATCATAATCTACCATTTTTAAACATTAAATATGGTTCATTTAATTTTTACAATGAATATATTGAAGCAGTTAGTCCATTAGAATTTCAAAAATTTACTAACTGA", "aa": "MLAAATAITLGSLYGCAKNSDEKLGKAHTGNPKDFKNYFVSVDKAESNFVTTDKSKKVAKFDFAKNQVIKENGQAISVDEFLDQYYREHHNLPFLNIKYGSFNFYNEYIEAVSPLEFQKFTN", "aa_hexdigest": "9d12f38d8e66c3a7f8be3ad04c7937f9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13865.381299999992, "isoelectric_point": 6.824737358093262 }, "id": "OAIKOHHJFB_505", "locus": "GCA000209735_00479" }, { "type": "cds", "sequence": "contig_1", "start": 259982, "stop": 260290, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATGGAAATAGCATTACTTTAGGTGCTCACTCAAGTAAAAATAAAGAGTTAACTACTATTAAATTCTACCCAGATGCTTTCTTTGGAACACTACCAATTTACTCAGAATTAAGTGGAGCTGGTAATGCTCAAGATTCTTTAGTTTATAAAGTAAACCAAAAACTTTTAACATCAGAACAGTTAAAAGATTTTTTGGCTAACATTTCAAATTACAATGCAATTAGTAATTATTCAAAAACAACTTTAGACAATTATGGTTTTAGAAATCTTCTTGATTATAGAACATTAATTAATCAAAAAGTTTGA", "aa": "MHGNSITLGAHSSKNKELTTIKFYPDAFFGTLPIYSELSGAGNAQDSLVYKVNQKLLTSEQLKDFLANISNYNAISNYSKTTLDNYGFRNLLDYRTLINQKV", "aa_hexdigest": "d236ab7c6e2c75a156e90a3fe3e31119", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11459.742699999986, "isoelectric_point": 8.860808753967284 }, "id": "OAIKOHHJFB_506", "locus": "GCA000209735_00480" }, { "type": "cds", "sequence": "contig_1", "start": 260562, "stop": 260660, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAGTTACTTAAATATTACTTTTGAAGATGGAACAAGTTTTGATGTTTTTAATAATTTTGAAGATATTACTCATAAAAATCCTAAAAATGAATGA", "aa": "MDSYLNITFEDGTSFDVFNNFEDITHKNPKNE", "aa_hexdigest": "cb32795d8e0fccf327f9b3ecb853fab6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3783.0048000000006, "isoelectric_point": 4.279487419128418 }, "id": "OAIKOHHJFB_507", "locus": "GCA000209735_00481" }, { "type": "cds", "sequence": "contig_1", "start": 261918, "stop": 262340, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTCAAAAAGAATTAAAGGTTTATTTACACCATTTATAACTAAAAATGAAACTCTTCCAGAAAACTATTTAATTTTAGGAAATCATGGTGATTACCCTCTTACTAAATTTAACATCATAGAAAAATTTGATCAATATGATTCAAAAAATATACTTGAATACATTGATATTGTTTACAATGGATTATCAACAGCAACCAATCCTCATCATAAAGAAGGCTACAAAAAATATTACACTAGAATTGAAGAAAAAATTAAAGCTGGAGATGCTACAGCTTTTAATCTTTATTCACAACTTAAAGCATTGCAAGTTAAACGTTTTGAGATTATAGAAAAGCAAAATGATCCTAAAGAAGACAAAACTTTATTGCAAGCTCAATTGCTATCAATCACTTATCAATTTGACCAATTGAAAAAATAA", "aa": "MISKRIKGLFTPFITKNETLPENYLILGNHGDYPLTKFNIIEKFDQYDSKNILEYIDIVYNGLSTATNPHHKEGYKKYYTRIEEKIKAGDATAFNLYSQLKALQVKRFEIIEKQNDPKEDKTLLQAQLLSITYQFDQLKK", "aa_hexdigest": "a594f942a7a2dd69ad44d3979adbeac2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16442.641599999984, "isoelectric_point": 8.889432716369633 }, "id": "OAIKOHHJFB_508", "locus": "GCA000209735_00482" }, { "type": "cds", "sequence": "contig_1", "start": 262404, "stop": 262898, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTATGTAAACTCGATTTAAATCTTCAAACTGCGAAAAAAATAGCCGAACTTCATCAAAGTTTAATTAATGAATACAAACAAAAAAGACTTGAATTAGAAACAATTGAAAGAAAAATTGAGTTAGAAAACTTCAAAATCCTTTATATGACTCAAACAGCAAATTTTGCCGATCCTGTTGTTATTGAACAATACAATAGAATTCTTCATAAATTTGAAGAAAATATTAATACTCACATGCCGACTTATATTGCAAAAGTTGTTGATGAACAATTCAAAGCAGCAGATTTAAAAGGAAAACAAGTATTAGAAATACTTAAAAATCCTACAACTTATAATCAAGTATCAATTTTCATTTCGGAAATTGAAAAAACAAATCGATACTTTAAAGACTTTAAAAATAAAGTGTTATTATCTCAAGCTGAATTAGTTGCTAAAATTCAAGTTGAAGTTAATTCTGATGAAAATTTATATAATCTTAACAAAAAGTTTTGA", "aa": "MLCKLDLNLQTAKKIAELHQSLINEYKQKRLELETIERKIELENFKILYMTQTANFADPVVIEQYNRILHKFEENINTHMPTYIAKVVDEQFKAADLKGKQVLEILKNPTTYNQVSIFISEIEKTNRYFKDFKNKVLLSQAELVAKIQVEVNSDENLYNLNKKF", "aa_hexdigest": "540e9ee28e22a1aa6a8b723a4de1ee78", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19371.179500000002, "isoelectric_point": 7.757180976867676 }, "id": "OAIKOHHJFB_509", "locus": "GCA000209735_00483" }, { "type": "cds", "sequence": "contig_1", "start": 262962, "stop": 263384, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTTATGAAATTGAGGACAATAACAACAAAAAAATTTCTGTTACTAAAAAAGCAAAAGAAAGAATGTTCGATTCTTATGCACAAGAAGAAGATGAGTTGAATCAAGTTGCAACACATTTAACAAACAGAATTAGCACTCATCAAACTGTACTTGACAATATAAATGCAAAAATAAATCAATTACAAGTTGAATATAAAGATCCTAAAATAACTGCTGAAAGAAAAGTTGAAATAATTGAATTACTAAAACAAATAGCTAAACTTTATGACAATACCAAAACAGCTATTTATCATATAAATGATGTTTTAAGAGAAATTAAATTAGCAATTAGCAATTTATTAGAAATTAAACAAGGCATTACAAATGGTACGCACACATATTTGGATGCTTATGATTTAGATTCTAATTTATTGAGTTGA", "aa": "MTYEIEDNNNKKISVTKKAKERMFDSYAQEEDELNQVATHLTNRISTHQTVLDNINAKINQLQVEYKDPKITAERKVEIIELLKQIAKLYDNTKTAIYHINDVLREIKLAISNLLEIKQGITNGTHTYLDAYDLDSNLLS", "aa_hexdigest": "80eaa76e9341eaea77431378e92d201e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16194.129399999987, "isoelectric_point": 5.568140983581542 }, "id": "OAIKOHHJFB_510", "locus": "GCA000209735_00484" }, { "type": "cds", "sequence": "contig_1", "start": 263622, "stop": 264164, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATGTTGCTAATAAAATTGAAACTTCACTATATAAAGAAGCAATTAATAAAGAAATAGAATCAACACTTGCATATAAAGCAAGCGAAAATAGCATTAAAAACCAAATTGAATTTTTCTCTAATTTGATGCCAGATTATTCAAAAATTATTAATGGTTTAGCTAAAAATTCAGAAATTGGTAATATGATTCTCGGTTTAAATATCGAAGTGGAAAAAAGTTGTGCTAATGATCCTTTAATTAAAAAATTAAACAATGTTTTAAAAGACTTAGATGATCCAAATAGTGAAATCAATAAATTTAAAGAAAAAGCCCAAAATGCCTATAAAACAGCAGGATTGAAAGATCAATTGGATAATATTGAGTCTGAAAAAAGTAAATATGGAAAGATGACTGATTCATTTATTAAATTGTTAAATGATACTATTAAAGTCAAAATTTTATCTAATTATATTAAAAATGAAAGTATCGATATTGAATCTTATGTTAAAAATACAAATAATGAGGCAAGACTTGCTGATACAAAAGATGCTGTTACTTGA", "aa": "MDVANKIETSLYKEAINKEIESTLAYKASENSIKNQIEFFSNLMPDYSKIINGLAKNSEIGNMILGLNIEVEKSCANDPLIKKLNNVLKDLDDPNSEINKFKEKAQNAYKTAGLKDQLDNIESEKSKYGKMTDSFIKLLNDTIKVKILSNYIKNESIDIESYVKNTNNEARLADTKDAVT", "aa_hexdigest": "5e3c6fe1d93397057903f4bfbcf77360", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20293.77760000001, "isoelectric_point": 5.243760871887206 }, "id": "OAIKOHHJFB_511", "locus": "GCA000209735_00485" }, { "type": "cds", "sequence": "contig_1", "start": 264405, "stop": 264650, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTAAAGCTCATTTGGAAATTATTGATAAAAGTAGAATTGAAGCAATTGAAAAAAGTATGCCTAAATTAAATCAAGATGAAAAACTTGTTAAATATGAAGCAATTATTAATGAAGGTTTTCAAAAAACTATTGATAAACTTTATAACATTGATGAAAATCTAAAAACAATAAATCTTTTTGATAATGCTTTTGAGCGTATGGCTATTTCAAAAGATTTTATTGAAACAAGAACATATGATTGA", "aa": "MIKAHLEIIDKSRIEAIEKSMPKLNQDEKLVKYEAIINEGFQKTIDKLYNIDENLKTINLFDNAFERMAISKDFIETRTYD", "aa_hexdigest": "2b867cdb640047d6a05a2b41a46e878e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9596.920599999992, "isoelectric_point": 5.165266227722166 }, "id": "OAIKOHHJFB_512", "locus": "GCA000209735_00486" }, { "type": "cds", "sequence": "contig_1", "start": 265080, "stop": 265805, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAATAAAGCTGTTGATGATCATATGAAACAAACTGAAAAAATTTTAAAATATCAAGCTCCAATTTCAAATTTCTTCAATGAATTTAATAGAGAATACTTAACCTTATTAAATCCAAATATTGTTGCTTTTGAAAAAATAGCTAATTCTAAAATTGCTTCAATTAAAACAGCGATTGGCGAGTTAATTAATGCAAGAATTAGAGAAATCTTAATTTCAGGTCGCGAATTTCCATTATATGATTCAAAAAACAATGGATTTGAATACAAAGATGCTGACAGTATTATTGTTGCTAAAAGTAGAAGCGAATTAATTGATATTTTAAGCAAGAAAAAACTTGTTAATGCTAATGATAGTGTTGAAAAAATCAACAAAGATATTTACTTTGTAAGATTAAGTCATCTTAAAAAAGATGGTACAAAACTTGTTGTTACATTAAGAAATATATTAACAGATGAAAAAGAAATGTTTGCAAATGGCTACAGTGACAAATACATCAAATTTAACATTGATGCCAGTGTAAATGACAAACGTCTAAGTACCCTAAACGCCTTATTTACAGCCGCAAACTACAAAAAACAAGTTACTCCAACTCTTATTAGAGAAGAAGGAAACATCCGTGATGAAAATGGCAAAAGTGTTAAAGGTTACAGTGTCTTTGTTGATGCTTACCAAGACATGACTGAAAAACTCTTACATGAAGTTCCTTATGCTGGAGAATGA", "aa": "MFNKAVDDHMKQTEKILKYQAPISNFFNEFNREYLTLLNPNIVAFEKIANSKIASIKTAIGELINARIREILISGREFPLYDSKNNGFEYKDADSIIVAKSRSELIDILSKKKLVNANDSVEKINKDIYFVRLSHLKKDGTKLVVTLRNILTDEKEMFANGYSDKYIKFNIDASVNDKRLSTLNALFTAANYKKQVTPTLIREEGNIRDENGKSVKGYSVFVDAYQDMTEKLLHEVPYAGE", "aa_hexdigest": "333bc44d9304527628d94babca1320b2", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 27578.191400000025, "isoelectric_point": 8.748891639709473 }, "id": "OAIKOHHJFB_513", "locus": "GCA000209735_00487" }, { "type": "cds", "sequence": "contig_1", "start": 265929, "stop": 266294, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGACCCTAACTTCAAAGGTATTTCAACGGACTTCTTAAAATTTGTTGGAGCCCATGAATATGGTCACCATATTACTTTAAATGGAGCTCATGATTTAGGAAACAAAGGCAATAATCCTATTTATGTTTCAGCTTTAACTCCAGGTGCTACGCCTCATATTGACAACTACTATTCAAAAGATGCTCTTGAACTTTATCTTAAAGCTAGAACACACTTAGAATTAAATACAACTCGTTTGTTAAAAGAAAACAACGTTGTAATTGACTATGGTGAATATATTACTTTCGGTATTCCAAAAATTGAAATAGTTAATGGTAAACCAAACTTAATTTATAAAAATGAAAATGAAGAAAACATTTGA", "aa": "MTDPNFKGISTDFLKFVGAHEYGHHITLNGAHDLGNKGNNPIYVSALTPGATPHIDNYYSKDALELYLKARTHLELNTTRLLKENNVVIDYGEYITFGIPKIEIVNGKPNLIYKNENEENI", "aa_hexdigest": "e3d3f63e6fd1a4e7ad53e1f909ebee3d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13631.155199999985, "isoelectric_point": 5.810559272766113 }, "id": "OAIKOHHJFB_514", "locus": "GCA000209735_00488" }, { "type": "cds", "sequence": "contig_1", "start": 266355, "stop": 266534, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAAACTTCAAAGGACTTCTTGAAGCGGTTATTGCTCGTCGTCAAGCAAATGGTTTAGATGACGATTATGAAAAATATCTAACTGCTTTTGATCTTTGGTTAATGAACTCACTTGACCATCATTCAGGTACAATTAATCCATCATTAAGAGGCGGCACAGCTAAATATATGGTTTGA", "aa": "MQNFKGLLEAVIARRQANGLDDDYEKYLTAFDLWLMNSLDHHSGTINPSLRGGTAKYMV", "aa_hexdigest": "40be366d4999376e777c12fbbec82708", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6687.509600000001, "isoelectric_point": 5.999548912048339 }, "id": "OAIKOHHJFB_515", "locus": "GCA000209735_00489" }, { "type": "cds", "sequence": "contig_1", "start": 266715, "stop": 266978, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAATGAAGATGGTACACCAGTTATTAATGTTCCATTGCATGTTGACTTTAATGACAAAAGCTCACCTTATTATGATGCAAATGCTATTAAATATGTAAATAGTAAAATTAAAGCTATTATTGAAACAATTAAAAGTTTAATTGTTGAAAAATACAGCATTAATGGTTGGGATACAACAGCTACTGATTTAAGTCTTGAACCTAAGTTTATACTTTCTAATTTATATTTAAGTACTTTACTTGGCAAAAATGATGACTGA", "aa": "MFNEDGTPVINVPLHVDFNDKSSPYYDANAIKYVNSKIKAIIETIKSLIVEKYSINGWDTTATDLSLEPKFILSNLYLSTLLGKNDD", "aa_hexdigest": "40c2b22ba963ea8f116b9e1fcfc22e45", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9784.96219999999, "isoelectric_point": 4.748409080505371 }, "id": "OAIKOHHJFB_516", "locus": "GCA000209735_00490" }, { "type": "cds", "sequence": "contig_1", "start": 267045, "stop": 267389, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGATGATTCAAAACCACTACCTTATTTTGATAATAGTGGTAAGCTAATTTATCCATCAAATAAAAAAATACCTCAAGCTTCAATTTATGCGAATATTAAAAAATATGCATTAGGAATTAAGCGTCCCGAAGATTTAACAAGTTTAATGTTTGCAATGAATACAGCAGGTGATTCATTTAATAATGTAGTTAATAGTGGTGAAGGTCACATTCTTTATGTAGATAAAGACCATCAATATTTACCAAATATTAATTTAGCTCAAACATTTGAAAATAACTTTTTATACAATTATGTTGCTGATATGGCGGACGTATTACAACTTAAAAAAATAATGAAATGA", "aa": "MIDDSKPLPYFDNSGKLIYPSNKKIPQASIYANIKKYALGIKRPEDLTSLMFAMNTAGDSFNNVVNSGEGHILYVDKDHQYLPNINLAQTFENNFLYNYVADMADVLQLKKIMK", "aa_hexdigest": "fc7fa4955eac2188585ff404244219ba", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12968.71679999999, "isoelectric_point": 6.711570930480956 }, "id": "OAIKOHHJFB_517", "locus": "GCA000209735_00491" }, { "type": "cds", "sequence": "contig_1", "start": 267516, "stop": 267707, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAATTAATGAAAAAATTATTGAAAAAGATCCTGTCTTAAGCTTATTTAATGCCTTTTATTCAATTGAAGGTAGTCAAAAATTATTTGGTAAAGATGTTGCATTTACTGATTACAAAAAATTCCTTGAATTTGTCTCAGTAGACTTAAAACAAGCCAAATTGGACAAAAACACTAAAGTAGTAAATTGA", "aa": "MSINEKIIEKDPVLSLFNAFYSIEGSQKLFGKDVAFTDYKKFLEFVSVDLKQAKLDKNTKVVN", "aa_hexdigest": "57091bbb1cd30df09ed7768297cc6004", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7233.293100000003, "isoelectric_point": 7.942972373962403 }, "id": "OAIKOHHJFB_518", "locus": "GCA000209735_00492" }, { "type": "cds", "sequence": "contig_1", "start": 268170, "stop": 268472, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGAAAGTAAAAACTCAGTTTATGGTCTTTTATTAACACTTGGTAAAGGTATATTCAAAAAAGCAAAACCTTCTCAAGATGTTCTTGATTACTACGGTTCAAAAACTGAACGTAAATTTAATGAATTTTTTACAGACTATACATATAACTTTGCAGAGGTTATTAACCGTGACAACTTACAAATTACTTATAGTCCATCAAATTCAAACTTTGGTAACATGCCTTCATATTTAAGTAATTTAAGTGAATCAAATACTGGTCTTGAATATGTTGTTGATGGGGCTGCAACTTCTAGATGA", "aa": "MLESKNSVYGLLLTLGKGIFKKAKPSQDVLDYYGSKTERKFNEFFTDYTYNFAEVINRDNLQITYSPSNSNFGNMPSYLSNLSESNTGLEYVVDGAATSR", "aa_hexdigest": "95284cf6269465cfaa8a247f74256388", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11276.379299999991, "isoelectric_point": 5.302987098693847 }, "id": "OAIKOHHJFB_519", "locus": "GCA000209735_00493" }, { "type": "cds", "sequence": "contig_1", "start": 268548, "stop": 268694, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAATGAAGAAGCAAGAAGAAGATCTCAAATTCTTAATACTAGATTTATTGAAAGAGTATTTGCAAATCAAGACAATATGCAATATGGCCAAAATTATAAAAATAATTACTTTGGACAATTTAAAACTATTAATAATGGTTGA", "aa": "MLNEEARRRSQILNTRFIERVFANQDNMQYGQNYKNNYFGQFKTINNG", "aa_hexdigest": "1dfe9e9dded48c15f5990b26ac6cfcd1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5833.407800000001, "isoelectric_point": 9.812748908996582 }, "id": "OAIKOHHJFB_520", "locus": "GCA000209735_00494" }, { "type": "cds", "sequence": "contig_1", "start": 268911, "stop": 269165, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGGTTACTTATCAACTGCAATAGCTGATAAAGCAAATTATTTAGCATTCAAAAATGTTAGAACAGGCGAAGTAAAAACGCTTCGCATTACAAAACAAAATGCAAGCAATATGTTCTATTACAAAGAACAACAAGTTAATAATGAAATTCGTCACCAAAATGGGGAAAAAGTACGTCACTACTTAATTGATGAACCATTTGATTTTACAGATAAAAATGGTCATCAAAAAGGTACTGGATTTGTTGCTTGA", "aa": "MFGYLSTAIADKANYLAFKNVRTGEVKTLRITKQNASNMFYYKEQQVNNEIRHQNGEKVRHYLIDEPFDFTDKNGHQKGTGFVA", "aa_hexdigest": "87fccb8041248ebca426ed11b6fc2cd4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9747.825799999997, "isoelectric_point": 9.259610176086426 }, "id": "OAIKOHHJFB_521", "locus": "GCA000209735_00495" }, { "type": "cds", "sequence": "contig_1", "start": 269184, "stop": 269291, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTAGATATAGAAATAGATTACTTGATACAAATGAAGAATATTATGTATATTTTGCTTCAGATGAACAAGGAAATAAAGCCTTAGAAGCAGACTTAGGAACCTGA", "aa": "MSRYRNRLLDTNEEYYVYFASDEQGNKALEADLGT", "aa_hexdigest": "45c3ce5d1dbd9e45a270f0d42f4d2ea9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4133.422600000001, "isoelectric_point": 4.440682792663575 }, "id": "OAIKOHHJFB_522", "locus": "GCA000209735_00496" }, { "type": "cds", "sequence": "contig_1", "start": 269438, "stop": 269923, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGAAATAAATTAATATTATCTTTAGCTAGTTTAAGTACTATTAGTACTGCCAGCATAGTAGCTGTTTCATGTGGTAATAAAGCATATAAAACAAACTATGATCTTGGTCTAGTAACTGAACCTATTAACTCATTAAATTACTTGAAATTTAACTCAGTTTCAAAAGTTTTACCTTCTATTGTAGAAAGTCCATTAAAAACAGGTCCTAATGAAGCTCTTAAAAGAATTTTAAGTTTGCCAGAAATTCCTATGGGAGTTTATGGTAATGATGACAAAAGTAATTCTATGGATGATTACATAGCAAATAATCGTGCTCCAAAAGAAGCATCTGGTCGTTTTTATCCTCTAGATCAATTTGGTTCTGCGCCAGGAACAATTAACACTGACCGTTCTGAGTATCAACCTGTTAGTGTTGTTATGACTAAAAATAATAAAATCCTTTCACTTCATATAACTTTAAATAGAGGCGAAAGTAGATGA", "aa": "MKRNKLILSLASLSTISTASIVAVSCGNKAYKTNYDLGLVTEPINSLNYLKFNSVSKVLPSIVESPLKTGPNEALKRILSLPEIPMGVYGNDDKSNSMDDYIANNRAPKEASGRFYPLDQFGSAPGTINTDRSEYQPVSVVMTKNNKILSLHITLNRGESR", "aa_hexdigest": "00fcbe3ecaa3d9c41544572d195dd2dd", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17610.908699999993, "isoelectric_point": 9.324529838562015 }, "id": "OAIKOHHJFB_523", "locus": "GCA000209735_00497" }, { "type": "cds", "sequence": "contig_1", "start": 269966, "stop": 270142, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATTACATTCTTGATATTAATACTGGTAGTCAAAAACAAACAAACATTTTGCAAAGAAAATTTAGATCAAGCAGTAGCCTTATTGATGCTCAACAAGAGTACATTAAAAAACACAAAAAGGCTTATTCAAATCCATTTGCTTATCCAAAATTAATTAAAGAAAATGGACAATGA", "aa": "MHYILDINTGSQKQTNILQRKFRSSSSLIDAQQEYIKKHKKAYSNPFAYPKLIKENGQ", "aa_hexdigest": "c4826ade9e167ede28aa5312607bf347", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6799.682800000002, "isoelectric_point": 9.85201015472412 }, "id": "OAIKOHHJFB_524", "locus": "GCA000209735_00498" }, { "type": "cds", "sequence": "contig_1", "start": 270353, "stop": 271036, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTACCTAATCCAGAATATTCATTAAGTCGTCACACAGCTGAAGAATTAAAATTAATTCCTCAAAGAATTGCAACAAAAATTAGAAAATATCTTTATTTTGATCCTCGTCAAAGTTATTCTGAAACAAAATTTAAGCCTTTAGTTAAAAAAGCTAAAAAGCTTAAAAGCAGAATGAACAAAAATGTATCTTTTGAAAAAGATATTAATGAATACAACCAAGAAGTTAACAAACTTTATGGCAAAGAAAATACTTTAAATAATAACTCAATTGACTCAAGAGAATTTATGGAAAATAGGGTACTTGCTCTCGATGAATTTTCACTAAGAGTTGAATATGATAGCAATGAACCTACTTCTCTTTCAAATGCCTACAGCGATATTCAATCAACATTAATTCCAGTTAATAGAAAATTTGTTGAAAGTATTGGTGGCATTAGAGAATTCGGTTTAGATAAATCTAAATTTTTAACTAATGGCCCTTTCTATATTAAAAATATAGTTTTAGGTCCTCAAGGTTATATGGAATTAGTTAAAAATAATACTTATTATTCATCTACTAAAACAATTAGTGATTCAATTAAAATCTACTTTAGCTCAGATGCAAATATTAATTCAGCTATGTATGATGATGGTTATATAGCTGCAACTAAAATTCCAGCTGTTCAACAAATTAATTATTGA", "aa": "MLPNPEYSLSRHTAEELKLIPQRIATKIRKYLYFDPRQSYSETKFKPLVKKAKKLKSRMNKNVSFEKDINEYNQEVNKLYGKENTLNNNSIDSREFMENRVLALDEFSLRVEYDSNEPTSLSNAYSDIQSTLIPVNRKFVESIGGIREFGLDKSKFLTNGPFYIKNIVLGPQGYMELVKNNTYYSSTKTISDSIKIYFSSDANINSAMYDDGYIAATKIPAVQQINY", "aa_hexdigest": "1897cee0dcccce0a62f0b090b7ea841e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26218.381600000015, "isoelectric_point": 9.070331001281737 }, "id": "OAIKOHHJFB_525", "locus": "GCA000209735_00499" }, { "type": "cds", "sequence": "contig_1", "start": 271061, "stop": 271210, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATCTTCAGGTTTTGGTACTATAGCCCTTGCCTTTAATTTGGATCAAGAAACCAATAAAAATTCTTTAATTAATGATGTTGATTTACGTAATGCCCTATATTTTGGAATTAATCGGAACGAAATGCTAAACATAGTTGGTTGA", "aa": "MKKSSGFGTIALAFNLDQETNKNSLINDVDLRNALYFGINRNEMLNIVG", "aa_hexdigest": "3d45a261e11820698e48f301f580578c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5461.146999999999, "isoelectric_point": 6.0008562088012685 }, "id": "OAIKOHHJFB_526", "locus": "GCA000209735_00500" }, { "type": "cds", "sequence": "contig_1", "start": 271304, "stop": 271771, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACAAAAGTTTCTGACAAAAAAATTCCAATTCAAAACTATAATCACGTTGACCACTTAGCAAAACAATACAATAATGAACATGTTGATAGAACTGATTTAGGTTATAACTTAGAAATAGCTAGAGCTTACATGCAAAGATTCAAAGATAAACATCCAGATGTAAAACAAGTAACTCTTAAATATATTTCTAACTCAACTGATGAACAACAAAATGCAGGTATTGCACTTAAAGACTTTATCAAAAAAGCTTTTGGTGACTACTTAATTATTGATGTTCAAGGTTTACCTGAAAACGTTTATGAAGACTTTAGAACTACAGGTAAATATGATCTTATTTATAGAAACTTTGATACTTTTGGCTCAGATAGTTATAGTTATGTTAAAGTCTTCTTCAAACCAGATGAAATTGATAGAAAGAATCAAAAAACCACTGGTTTTAGAAACAACCCTTCTGGTAGTTGA", "aa": "MKTKVSDKKIPIQNYNHVDHLAKQYNNEHVDRTDLGYNLEIARAYMQRFKDKHPDVKQVTLKYISNSTDEQQNAGIALKDFIKKAFGDYLIIDVQGLPENVYEDFRTTGKYDLIYRNFDTFGSDSYSYVKVFFKPDEIDRKNQKTTGFRNNPSGS", "aa_hexdigest": "10a1e4ba01d5dafbc5d51422879e05b8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18135.033199999983, "isoelectric_point": 8.583530235290528 }, "id": "OAIKOHHJFB_527", "locus": "GCA000209735_00501" }, { "type": "cds", "sequence": "contig_1", "start": 272258, "stop": 272371, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAAAGAAATGATCAAAATTATTTCACTGGCACAGTAAATAAAGATCAATATAATCTTTATAACCGTGAAGTTGATTTTAGCGGTAAAGCTAAAAAAGATAATAGATGA", "aa": "MQKRNDQNYFTGTVNKDQYNLYNREVDFSGKAKKDNR", "aa_hexdigest": "4d5062402735ed610fcae5a083d11ceb", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4486.849100000002, "isoelectric_point": 9.520900535583493 }, "id": "OAIKOHHJFB_528", "locus": "GCA000209735_00502" }, { "type": "cds", "sequence": "contig_1", "start": 272705, "stop": 273064, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAGGTATTTTCTCAGTATTCTTAACATTAATTATAGGAATTCCTGTTGGTGTTTATGTTGGTATGAATCCTGATAAATTACCAGACCATATTGCAACATTAATTGTTTCAGTATTCTCATCAATTCCATCATTAGTTTTTGCCCTATTGCTCTTATTAATAGGCCGAAGCTTAAATATGCCTTACTTGTTTAATGAGCTTGATATTGCTACATATGTATTACCAGGATTAGCCTTAAGTTTAGGTTCAATTATTGTTTATATTAAATATATTCGTACAGAATTAAATCGAGAATTAAATTCTCAACATGCTAAATTCTGCTACTTAAAAGGTTTAAGCAAACGTCGTTTTGTTTGA", "aa": "MIGIFSVFLTLIIGIPVGVYVGMNPDKLPDHIATLIVSVFSSIPSLVFALLLLLIGRSLNMPYLFNELDIATYVLPGLALSLGSIIVYIKYIRTELNRELNSQHAKFCYLKGLSKRRFV", "aa_hexdigest": "b5f7ee1c8f44c5e7baa8aa8fe17ce081", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13272.822699999979, "isoelectric_point": 9.41491451263428 }, "id": "OAIKOHHJFB_529", "locus": "GCA000209735_00503" }, { "type": "cds", "sequence": "contig_1", "start": 273143, "stop": 273325, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGTTGAGCAAATCTTCTTTATTACAGGTAGTGGTGGATTATTAATCCAAGCTATTACTTCAAAAGACTACAACATTATTCTCTTTATGGTTACATTATTCTCATTAATTACAATTCTTTCATACGCATGTCGTGATGCTCTTTACCAAATCATTGATCCTCGTGTGAGAAGAAGATAG", "aa": "MFVEQIFFITGSGGLLIQAITSKDYNIILFMVTLFSLITILSYACRDALYQIIDPRVRRR", "aa_hexdigest": "a44c8e6b125770220820a8f7800758e5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6946.2058, "isoelectric_point": 9.098052406311037 }, "id": "OAIKOHHJFB_530", "locus": "GCA000209735_00504" }, { "type": "cds", "sequence": "contig_1", "start": 273500, "stop": 273721, "strand": "+", "frame": 2, "gene": null, "product": "N-terminal TM domain of oligopeptide transport permease C family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAE2EIN1" ], "nt": "ATGCATAAACAAAAGAAACCATTTTTAGAATTCTTCTATCGTTTCAGTCGTTCAACTGCTGGAGTATTTGGTTTTGTGGCTCTTGCTTTATTAATTATTCTCGCAATATTCTTGCCTTTTACAACCAAAGATCCTAATACTGTTAATACTGAAAAAAGATATTTAATCTTCTTTACAGATGGACACATTTTTGGAACTGATGCTTTAGGCCGCGATATTTGA", "aa": "MHKQKKPFLEFFYRFSRSTAGVFGFVALALLIILAIFLPFTTKDPNTVNTEKRYLIFFTDGHIFGTDALGRDI", "aa_hexdigest": "76472b4940abb58951f87b0170df7f8a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAE2EIN1", "query_cov": 1.0, "subject_cov": 0.9733333333333334, "identity": 0.6579999999999999, "score": 102.0, "evalue": 2.4e-27, "valid": false, "product": "N-terminal TM domain of oligopeptide transport permease C family protein", "uniref50_id": "UniRef50_A0AAE2EIN1" }, "pscc": { "uniref50_id": "UniRef50_A0AAE2EIN1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAE2EIN1" ], "product": "N-terminal TM domain of oligopeptide transport permease C family protein" }, "genes": [], "id": "OAIKOHHJFB_531", "locus": "GCA000209735_00505" }, { "type": "cds", "sequence": "contig_1", "start": 274112, "stop": 274336, "strand": "+", "frame": 2, "gene": null, "product": "ABC transmembrane type-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PSU9" ], "nt": "GTGTTCCATATTCCAGGTGTTATTCTTTCAGAAACTTCTTTAGCCTTCATTGGTTTATCAATAACAGATATTCCAACATTAGGCAACTTAATCAATGATGGTTCAAAAATCTTTACTACTTTCCCTCGTTATGTTTTAATTCCTTCAACAATGTTAATCATTGTAACAACAAGTATTCAATTAATGTCAGCTGCAATTCAAGACAGCTTACTAAGACAAAGATAA", "aa": "MFHIPGVILSETSLAFIGLSITDIPTLGNLINDGSKIFTTFPRYVLIPSTMLIIVTTSIQLMSAAIQDSLLRQR", "aa_hexdigest": "ff062bc2a790fe596087ebfb724b0aab", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PGD7", "query_cov": 1.0, "subject_cov": 0.8809523809523809, "identity": 0.662, "score": 100.0, "evalue": 2.64e-26, "valid": false, "uniref50_id": "UniRef50_A0A3B0PSU9" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PSU9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PSU9" ], "product": "ABC transmembrane type-1 domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_532", "locus": "GCA000209735_00506" }, { "type": "cds", "sequence": "contig_1", "start": 274629, "stop": 275396, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDR3", "UniRef:UniRef90_A0A3P8MDR3" ], "nt": "GTGGCTGCTGAAATTGAAGATGTTTACTTAACATTTAGAAATCCAGCAGATCCTAAAGAAAAGAATCTTGTTTTAAGAGGACCTTCTTTAAAAATTTATGAAGGAAAAGTTCACGCCATCATTGGTGAAAGTGGTAGTGGTAAAAGTGTTATTACTTCACTTCTATATGGACTTACAGGTTCAAATGCCATTATTGAATCTGGAAAAATTAAACTTTATGGCTTAGAAGTTCAAGATTACACAATGTACAATTGGGAAAAATCTAAACTTAGAGGCCGTGTTGTTAGTGCAGTATTCCAAAACCCTATGTCAATTTTGGACCCAACTATGAAAGTTGGAAAACAAATTATAGAAGGAATGCTAATTAATAAAATAGTTAAAACTAAAAAAGAAGCCAAAGCAGAAGCTATTAAATATTTGGAAATGACTAAAATTAATAACCCACAAAAAGTTATGGATTTATATCCTCATGAACTTTCGGGTGGTATGATCCAAAGAATTGCAATTGCAGCTATTGTTTCATTGAAACCTAAAATTTTAGTCATGGATGAGCCCACAACAGCTTTAGACCCAACAGTCCAAGCTTTAGTTTTAGATATTATTAGGGAACTTCAAGAAAGATTCCATATTGCTATAGCATTCATTACTCACGACTTAGGTGTTGTTGCTTCAATTTCTGATTTTATCAACATCATGTATGCTGGTCAAATTATTGAAAGTGGAACAAAAGAAGAAATATTACAAAATCCACAACACCCTTATACTTGA", "aa": "MAAEIEDVYLTFRNPADPKEKNLVLRGPSLKIYEGKVHAIIGESGSGKSVITSLLYGLTGSNAIIESGKIKLYGLEVQDYTMYNWEKSKLRGRVVSAVFQNPMSILDPTMKVGKQIIEGMLINKIVKTKKEAKAEAIKYLEMTKINNPQKVMDLYPHELSGGMIQRIAIAAIVSLKPKILVMDEPTTALDPTVQALVLDIIRELQERFHIAIAFITHDLGVVASISDFINIMYAGQIIESGTKEEILQNPQHPYT", "aa_hexdigest": "fccfa6d2da0b76016ecd842b85b4407c", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A3P8MDR3", "query_cov": 0.8117647058823529, "subject_cov": 1.0, "identity": 0.9520000000000001, "score": 376.0, "evalue": 1.63e-130, "valid": true, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A3P8MDR3" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MDR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDR3" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "OAIKOHHJFB_533", "locus": "GCA000209735_00507" }, { "type": "cds", "sequence": "contig_1", "start": 275412, "stop": 275645, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LB07", "UniRef:UniRef90_A0A3P8LB07" ], "nt": "ATGCCTGACTTTAATAAAGGTACAAAACTTCAAGTTATTAGAGGTGCTGTTCCTTCAAACTTAAATAATATTGATGGTGATGCTTTTGCAGTTAGAAATGACTATGCTTTAGGAATAGATTTCGAAGAAGAATCACCTTTCTATCAAATCTCTCCTACACACTTTGTTAAAAGTAATTTGCTTAATGGACAAGCCCCAAAATATGAACCTCCAAAAATTATTCAAAACCTTTGA", "aa": "MPDFNKGTKLQVIRGAVPSNLNNIDGDAFAVRNDYALGIDFEEESPFYQISPTHFVKSNLLNGQAPKYEPPKIIQNL", "aa_hexdigest": "6d3971f89d03edccc517277886356ddc", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 275412, "stop": 275837, "observations": { "mutation": [ 275643 ], "stop": [ 275643 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8LB07", "query_cov": 1.0, "subject_cov": 0.5460992907801419, "identity": 0.857, "score": 135.0, "evalue": 2.2e-39, "gene_start": 1, "gene_end": 77, "reference_start": 1, "reference_end": 77, "reference_sequence": "MPDFNKDSKLQVIRGAVPSNLNNIKGDAFAVRNDYALGIDFEEESPYYQISTTHFVKSNLLNKNVSKYEPPKIIKRLWDKYENQMNSIYGDDKYVSQKVYDEFVQKAYENNELVKKLIAKREEEKAIKQSTEVKKPGEKNA" }, "paralog": false, "identity": 0.723404255319149, "score": 206.0, "evalue": 1.31e-69, "description": "Internal stop codon at 275643. Nonsense mutation around 275643.", "psc": { "uniref90_id": "UniRef90_A0A3P8LB07", "uniref50_id": "UniRef50_A0A3P8LB07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8LB07", "UniRef:UniRef50_A0A3P8LB07" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LB07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LB07" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8607.587299999994, "isoelectric_point": 5.115418434143066 }, "id": "OAIKOHHJFB_534", "locus": "GCA000209735_00508" }, { "type": "cds", "sequence": "contig_1", "start": 275830, "stop": 276171, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGCCTAAATATTACTACGAAAAAAGAAAATTTGGCAAACCACTTGCTAGAGAAATTAGACAAGGTACAGATGAAATGCTTAATGCATATCCAAATAAAAAAGCTTTAGTAGAAATAAGAAATTTAGATATTGATTATGGTTTTGGAGCTAAAAAATTTAGTGCTATAAAAGATTTAAATTTGAATATTTATGATGGCGAAGTTTTAGGACTTGTTGGTGAAAGTGGCAGTGGTAAAAGTACCACTGGTAGAGCAATTATTGGTCTAACACCACACACTTATGGATATATAAAAATCCTTGATAGAATCATTCCAAAAAACTTGGATAAAGGTTTCAAGTGA", "aa": "MPKYYYEKRKFGKPLAREIRQGTDEMLNAYPNKKALVEIRNLDIDYGFGAKKFSAIKDLNLNIYDGEVLGLVGESGSGKSTTGRAIIGLTPHTYGYIKILDRIIPKNLDKGFK", "aa_hexdigest": "377bf9bd8060109b3c9803d3fbd39afc", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 46, "aa_cov": 0.40707964601769914, "hmm_cov": 1.0, "evalue": 4.468533635631652e-12, "score": 42.85324478149414, "start": 57, "stop": 102 } ], "seq_stats": { "molecular_weight": 12730.593599999986, "isoelectric_point": 9.572088432312011 }, "id": "OAIKOHHJFB_535", "locus": "GCA000209735_00509" }, { "type": "cds", "sequence": "contig_1", "start": 276202, "stop": 277068, "strand": "+", "frame": 1, "gene": null, "product": "Oligopeptide ABC transporter, ATP-binding component", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MRZ9" ], "nt": "ATGGTTAATAAAGTACAAATGATTTTCCAAGATCCAACTAATTCACTTAACCCTTACAAAAATGTTGAAACAGTTGTTGGTGAAGGTTTAACAAACTTAAAGAACTCAAAATATATTTTCTTAACACGCTTTGATCAAATAGTTTTAAAAGAAGTAAATAGATTGATAAATAAAATTGATTCAACTAATTCTTTGATTTCTAATTTATTAGAAAAATGTCGTGAAATAGAAGTTTCAATTGAAAAATCTTATAACTTTGTTTTTAAAGAATTAATTGATGAAATTAATAGAAGATATACAACTAATGCAAATTACAAAGATGCTTTAGAATACTTAGAGTCTCAAAAAGTTGAAAGAGATTCATTAGATAATTTAAGTGAAAAAGAATGTAAAAAACGTTTAATCATTGACATTCTTAAACAAGTAGGTTTAGATGAAACTGTACTTGGTCGTTTCCCGCTTGAATTCTCAGGTGGTCAACAACAACGTTTAGGAATTTGTCGTTCAGTGGTTTTACAACCTAAATTATTAATCGCAGATGAGCCTATTTCAGCTCTTGACGTTTCTATTCAAGCTCAAGTTATTAACATTTTTAATGAGCTTAAAGAAAAATATAACTTAACAATTCTCTTTATTGCCCATGACCTTCGGATGGTTGAATATATTTCAGATAGAATAGCTGTTATTAATAAAGGTGTCTTACTTGAAGTAGGTCCAACTGATGAAATTATTCATAATGCTCATCATCCTTACACAAGAAGTTTATTAGATGCCGTTCCTTCTATTGAAAGTGAAAAAGGCTCATTATTAGGTTATATTTATGATCCAAGTATGCATAAATATGATGAAAATAATCAACCAGTTTGA", "aa": "MVNKVQMIFQDPTNSLNPYKNVETVVGEGLTNLKNSKYIFLTRFDQIVLKEVNRLINKIDSTNSLISNLLEKCREIEVSIEKSYNFVFKELIDEINRRYTTNANYKDALEYLESQKVERDSLDNLSEKECKKRLIIDILKQVGLDETVLGRFPLEFSGGQQQRLGICRSVVLQPKLLIADEPISALDVSIQAQVINIFNELKEKYNLTILFIAHDLRMVEYISDRIAVINKGVLLEVGPTDEIIHNAHHPYTRSLLDAVPSIESEKGSLLGYIYDPSMHKYDENNQPV", "aa_hexdigest": "acebac3e5e9c77a1189a8b17d93a7de6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q6MRZ9", "query_cov": 1.0034722222222223, "subject_cov": 0.903125, "identity": 0.6509999999999999, "score": 359.0, "evalue": 1.07e-121, "valid": false, "product": "Oligopeptide ABC transporter, ATP-binding component", "uniref50_id": "UniRef50_Q6MRZ9" }, "pscc": { "uniref50_id": "UniRef50_Q6MRZ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MRZ9" ], "product": "Oligopeptide ABC transporter, ATP-binding component" }, "genes": [], "id": "OAIKOHHJFB_536", "locus": "GCA000209735_00510" }, { "type": "cds", "sequence": "contig_1", "start": 277093, "stop": 277818, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAGCTACCGATGAAGAATTAGCTAAATACAAAAGAGATAGAACTAGACTTAATGAAATTGAAGCTAAAAGAGAAAGAAATGAAAAGATTACACTTGATGACTTTATCATTGAAGAAAATGTTATTAATGAACAAGAAAAATATGATCTTGTAACTAAAATTAAAGAAGCTCAAATTGACTTAAATAACTATAATGAAGGGTCAGAAGTAAAAATTACTCCTTCTTATGTTAAAACTGCTACTGTTATTCAAGAAGAAGTTTCAACTTATGATTCACTAAAAGAAACTACTAAATTAATTAAAGAAGATGACTTACCTGAAGTCAAAGTAACTCCTTCTTATATAGAAGTGGCTAAAGTTATTGAAGATGATGAAATTAAAAAAACCGAGAAATCTTCAACTAAAAAAGTTGAAAAAATTACAATTATTTCAAATGTTCACAAAGATTCAACTGTAGAATTAATTCCTGAAAATGATAAAATTACAGTAGAAGAAAAACCAAAAAAAGTTAGAAAAACTAGAAAGGTGAGTGCAACATTGAAAAAACCTACTAAAAAATCAACAACCAAAAAACAAACAAAAACATCTACAATTACTGTTGACTTATCAAAGAAAATCAAACCTAACAAAGGTGTTACAAAAGCTGAATTAAGAGCACAAGCAAGAAAAGAAAAATCTGCAAACAAAAAAATAGTAGAAAAACTTGCTAAAGAAAATGAATAA", "aa": "MLATDEELAKYKRDRTRLNEIEAKRERNEKITLDDFIIEENVINEQEKYDLVTKIKEAQIDLNNYNEGSEVKITPSYVKTATVIQEEVSTYDSLKETTKLIKEDDLPEVKVTPSYIEVAKVIEDDEIKKTEKSSTKKVEKITIISNVHKDSTVELIPENDKITVEEKPKKVRKTRKVSATLKKPTKKSTTKKQTKTSTITVDLSKKIKPNKGVTKAELRAQARKEKSANKKIVEKLAKENE", "aa_hexdigest": "8331c57e384b9c4550972a514e6a31a5", "start_type": "GTG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 27677.369600000064, "isoelectric_point": 9.201137351989747 }, "id": "OAIKOHHJFB_537", "locus": "GCA000209735_00511" }, { "type": "cds", "sequence": "contig_1", "start": 277855, "stop": 278259, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013354444.1", "SO:0001217", "UniParc:UPI0001A8C120", "UniRef:UniRef100_C4XEF3", "UniRef:UniRef50_C4XEF3", "UniRef:UniRef90_C4XEF3" ], "nt": "ATGGATAAGGCTAAGAAAAAGAAAATTTTAATTCCACTCTTAAGTACTGTTGGTGTTTTAGCTGTTGTTACTCCAATTGTTGTTGTATCTGCAGCTAAAAGTAAACATAAGGAAATTGAAAAACAAGAAACAATAAATTCTTTTGAAGAATTTCCTAAATTAGAATATAACAACTATTACAAGTACATTAGAATAGGTGAAAAAGGCGAAGAATATATTGATGAAAATGTTGTGCCTTTAATAGTGAAAGATGTTTTTCAAAAACTAAACGCAGATTATCAAAGTAAAATTTCTTTTGATTATCAATTTGTAAGCAAAAATCATTTAGAACTTAAATTTAAATATGTTTCAGAAGACAAAACACTTTTCAAAACTTATGTTTTAAAAACTAAAAATATGGTTTAA", "aa": "MDKAKKKKILIPLLSTVGVLAVVTPIVVVSAAKSKHKEIEKQETINSFEEFPKLEYNNYYKYIRIGEKGEEYIDENVVPLIVKDVFQKLNADYQSKISFDYQFVSKNHLELKFKYVSEDKTLFKTYVLKTKNMV", "aa_hexdigest": "b103e33d00b36b030ab5faad621b356a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001A8C120", "ncbi_nrp_id": "WP_013354444.1", "uniref100_id": "UniRef100_C4XEF3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C120", "RefSeq:WP_013354444.1", "UniRef:UniRef100_C4XEF3" ] }, "ips": { "uniref100_id": "UniRef100_C4XEF3", "uniref90_id": "UniRef90_C4XEF3" }, "psc": { "uniref90_id": "UniRef90_C4XEF3", "uniref50_id": "UniRef50_C4XEF3" }, "pscc": { "uniref50_id": "UniRef50_C4XEF3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEF3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15662.085599999993, "isoelectric_point": 9.171546363830569 }, "id": "OAIKOHHJFB_538", "locus": "GCA000209735_00512" }, { "type": "cds", "sequence": "contig_1", "start": 279122, "stop": 279589, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCATGATATTTCGCAAGAACGTTATGTATACAAATCAGAATATTTAGATGCTCCATCTTATTTAACTAAAGATCACTATGATGTAAATTTTAAAGATGCATGCAATATTGTAAATGGTAATATTGAAATAAAAAGATATTTTACAGAAGATAGATTTGCAATTAAAATATTTTTTATTCAACCCAAAAATTTACATAAAAAATTCTTTGAAGGTTTTGATATTGAAATAAATGGAAAAAGAATTGATGGAAACATAATTAGAAGCTGCACAAAATTGGAAACAATGGATAGCAGTTTACTAAATGAAAGACTTAGTTTTAAAAAATGGTATTCGAAATTTACTGAAGAAACAAGTGCATATGGATTAACAATCCTTTCACCTAAAATTATAAATTTACCTTACGAAAAATTCCGTATTTTAAGAGTTTACTTTAGAAGTAAAGAAAAAACAGAAAAACAATATTGA", "aa": "MHDISQERYVYKSEYLDAPSYLTKDHYDVNFKDACNIVNGNIEIKRYFTEDRFAIKIFFIQPKNLHKKFFEGFDIEINGKRIDGNIIRSCTKLETMDSSLLNERLSFKKWYSKFTEETSAYGLTILSPKIINLPYEKFRILRVYFRSKEKTEKQY", "aa_hexdigest": "b7f7660e82ca0611824517e5a4888c5a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18660.153599999998, "isoelectric_point": 9.052537727355958 }, "id": "OAIKOHHJFB_539", "locus": "GCA000209735_00513" }, { "type": "cds", "sequence": "contig_1", "start": 279702, "stop": 279959, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEF5", "UniRef:UniRef90_C4XEF5" ], "nt": "ATGAATCTAACTTTTAATGATTATTTTATGGGCTTAATTTCACATAAAGATCAAAATAGTGTTTTACACAATATCTTTAAAATGGAAAAAGTCAATGAGCAAGCTTACAAAAAGACAATTGGAGGAGGCAACAAATCAAACATACTTAAAAACATCTTTAAACCAAAAAATAAAAGTCAGCATATTTTATCTATTATGAAACCTGAACTTGCGCAAATTATCAAAGAGGATTTCTTAAAAAGTCAATCTAAAAATTGA", "aa": "MNLTFNDYFMGLISHKDQNSVLHNIFKMEKVNEQAYKKTIGGGNKSNILKNIFKPKNKSQHILSIMKPELAQIIKEDFLKSQSKN", "aa_hexdigest": "622a308b90e7224fe28346681ade097f", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 279615, "stop": 279974, "observations": { "mutation": [ 279704 ], "stop": [ 279704 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEF5", "query_cov": 1.0, "subject_cov": 0.7142857142857143, "identity": 1.0, "score": 168.0, "evalue": 1.75e-52, "gene_start": 1, "gene_end": 85, "reference_start": 6, "reference_end": 90, "reference_sequence": "MERRKMNLTFNDYFMGLISHKDQNSVLHNIFKMEKVNEQAYKKTIGGGNKSNILKNIFKPKNKSQHILSIMKPELAQIIKEDFLKSQSKNWFKDYYSKNTYYKYKKQAVEEFLYHYFNE" }, "paralog": false, "identity": 0.9831932773109243, "score": 240.0, "evalue": 1.88e-83, "description": "Internal stop codon at 279704. Nonsense mutation around 279704.", "psc": { "uniref90_id": "UniRef90_C4XEF5", "uniref50_id": "UniRef50_C4XEF5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEF5", "UniRef:UniRef50_C4XEF5" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEF5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEF5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9839.358899999996, "isoelectric_point": 9.778387260437011 }, "id": "OAIKOHHJFB_540", "locus": "GCA000209735_00514" }, { "type": "cds", "sequence": "contig_1", "start": 280142, "stop": 280342, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAATTAGAGTTGATAAAGATTCCTTATTAAAACAAGCTGAATTTAGTCATGTTTCTCAAGATGAATTTAAAGACTATCATAAAAATATTATTAATAATACTTTCCCTTTAACTATTGGCGGTGGTATAGGGCAAAGTAGACTTTCAATGTTCTTGTTAGAGAAGAAACACATTGGTGAAGTTCAAGTAGGTATTTGA", "aa": "MGIRVDKDSLLKQAEFSHVSQDEFKDYHKNIINNTFPLTIGGGIGQSRLSMFLLEKKHIGEVQVGI", "aa_hexdigest": "28e07f933ce3c23cef55071f2989b38e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7429.448299999997, "isoelectric_point": 6.820531272888183 }, "id": "OAIKOHHJFB_541", "locus": "GCA000209735_00515" }, { "type": "cds", "sequence": "contig_1", "start": 280406, "stop": 280675, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03590.20" ], "nt": "TTGAAAAAAATAGTCAACACAATTTTTAAATGTATTCGTCATGTTGAATACAAACTTTTAGTTGAATTTCCACAACTAAGTCAAAAATTACCTGAAAATGTAACTTTTATTAGTAGTAGTGAGCTTTACAACTTATATCCAAAATTAAGCCCTGAAGAAAGAGAAACAAAATTTGCTAAAAAACACAAGGCTATATTTATTTATGAAATTGGTTATCCTTTAGCAGATAATAAGCCTCAATCAAAACGTGCTTTTGACTACGATGACTGA", "aa": "MKKIVNTIFKCIRHVEYKLLVEFPQLSQKLPENVTFISSSELYNLYPKLSPEERETKFAKKHKAIFIYEIGYPLADNKPQSKRAFDYDD", "aa_hexdigest": "94c5f14c16e70b051e30b92ae4251115", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03590.20", "name": "AsnA", "length": 89, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.308472290053367e-29, "score": 99.6573486328125, "start": 1, "stop": 89 } ], "seq_stats": { "molecular_weight": 10573.116699999986, "isoelectric_point": 8.967632865905763 }, "id": "OAIKOHHJFB_542", "locus": "GCA000209735_00516" }, { "type": "cds", "sequence": "contig_1", "start": 280841, "stop": 281071, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03590.20" ], "nt": "ATGTACAAGAGTAAATTAAACATTAAAGAAACTCAATTAGCAATTCAAGATTTGAAGTTTGCATTTACTAAAAAATTAAACAAGGAATTACATTTAACTAGAGTTTCAGCCCCTTTATTTGTTGCTAGAAATACCAAAATAAATGATGGCTTAAATGGGGAAGAACCTGTATCTTTTGTTCCTAAAAATTTTGATGAAACTGTTGAAATAGTTCACTCTCTTGCTAAGTGA", "aa": "MYKSKLNIKETQLAIQDLKFAFTKKLNKELHLTRVSAPLFVARNTKINDGLNGEEPVSFVPKNFDETVEIVHSLAK", "aa_hexdigest": "fd57b4d2e512df91a6ada4ac12db9f79", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03590.20", "name": "AsnA", "length": 67, "aa_cov": 0.8552631578947368, "hmm_cov": 1.0, "evalue": 2.3671422080272484e-19, "score": 66.10094451904297, "start": 12, "stop": 76 } ], "seq_stats": { "molecular_weight": 8657.9479, "isoelectric_point": 9.347029304504396 }, "id": "OAIKOHHJFB_543", "locus": "GCA000209735_00517" }, { "type": "cds", "sequence": "contig_1", "start": 281058, "stop": 281219, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCATTGTTAATTTACATATCACAATTATCAATGATGTTACACATCTTGAAAACAACAAAAAAAGCTATGCAAGAAGCTAAAAATAATCAAGAAAATTCAATTCCACAAAATGACCAAAACAACTATAAAACAGAGGCTAACGAAAATGTACAAGAGTAA", "aa": "MSLLIYISQLSMMLHILKTTKKAMQEAKNNQENSIPQNDQNNYKTEANENVQE", "aa_hexdigest": "d104dafb46ec731f98f5447d9804ae5b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6168.919100000002, "isoelectric_point": 5.697790718078613 }, "id": "OAIKOHHJFB_544", "locus": "GCA000209735_00518" }, { "type": "cds", "sequence": "contig_1", "start": 281298, "stop": 281657, "strand": "-", "frame": 3, "gene": null, "product": "PQ loop repeat", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEF7", "UniRef:UniRef90_C4XEF7" ], "nt": "ATGGCGGCTGTAGTTTATGCCAATATTTTGTGTGCTTATGTCTATTGTGTACTTTTATTCTTTATGTACAAATATGATGAAAAAGTAAAAAGAAACAAAAGAAAATTTGTAGTTTTAGGAATAACATTAACTTTAACTACAATTTGTGCAATTGTTGGTTTTGTCGGTTTATATCACAAAAACAGTGAAGGCAATGGTCTTAATTTTGATCCTAATACAACTGCTATTTTGTCTCAAATTATTCCAGTTATATCAACTTTTGCATTCTTACCACAAGTATTAAAAACATTTGAAAAGAGAGATATTGAAGGCTTATCAGTTGGTTTAATTTTAATGTTTGTATTAACAAATATTTTTTGA", "aa": "MAAVVYANILCAYVYCVLLFFMYKYDEKVKRNKRKFVVLGITLTLTTICAIVGFVGLYHKNSEGNGLNFDPNTTAILSQIIPVISTFAFLPQVLKTFEKRDIEGLSVGLILMFVLTNIF", "aa_hexdigest": "c7f5c9bfba956729557ae0903bac8929", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [], "truncated": "both", "pseudogene": { "start": 281058, "stop": 281897, "observations": { "mutation": [ 281228, 281288, 281300, 281696, 281723 ], "stop": [ 281228, 281288, 281300, 281696, 281723 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEF7", "query_cov": 1.0, "subject_cov": 0.4265232974910394, "identity": 1.0, "score": 233.0, "evalue": 1.6e-75, "gene_start": 1, "gene_end": 119, "reference_start": 81, "reference_end": 199, "reference_sequence": "MDFIRSSFAINKDVNDGIAAFVAIVGMIACIATISVSIPQLVYLLKRKKTGEVKFYSFWIFFAALCSWIIFGSFTGEEAKMAAVVYANILCAYVYCVLLFFMYKYDEKVKRNKRKFVVLGITLTLTTICAIVGFVGLYHKNSEGNGLNFDPNTTAILSQIIPVISTFAFLPQVLKTFEKRDIEGLSVGLILMFVLTNIFWIAYWVSLIIAEGKLTPALTSTLLWQTLSLLIYISQLSMMLHILKTTKKAMQEAKNNQENSIPQNDQNNYKTEANENVQE" }, "paralog": false, "identity": 0.982078853046595, "score": 523.0, "evalue": 3.89e-192, "description": "Internal stop codon at 281228, 281288, 281300, 281696, 281723. Nonsense mutation around 281228, 281288, 281300, 281696, 281723.", "psc": { "uniref90_id": "UniRef90_C4XEF7", "product": "PQ loop repeat", "uniref50_id": "UniRef50_C4XEF7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEF7", "UniRef:UniRef50_C4XEF7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEF7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEF7" ], "product": "PQ loop repeat" } }, "id": "OAIKOHHJFB_545", "locus": "GCA000209735_00519" }, { "type": "cds", "sequence": "contig_1", "start": 281721, "stop": 281897, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTTATACGTAGTTCTTTTGCTATAAATAAAGATGTTAATGATGGCATAGCAGCGTTTGTAGCAATAGTAGGAATGATAGCATGTATTGCAACAATAAGTGTATCAATTCCTCAATTAGTTTATTTATTAAAAAGAAAGAAAACTGGGGAGGTTAAATTCTATTCATTCTGA", "aa": "MDFIRSSFAINKDVNDGIAAFVAIVGMIACIATISVSIPQLVYLLKRKKTGEVKFYSF", "aa_hexdigest": "996b09043a343c4a26662b57c1bf715b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6401.559499999999, "isoelectric_point": 9.35966510772705 }, "id": "OAIKOHHJFB_546", "locus": "GCA000209735_00520" }, { "type": "cds", "sequence": "contig_1", "start": 282138, "stop": 282563, "strand": "+", "frame": 3, "gene": null, "product": "Gamma-glutamylcyclotransferase AIG2-like domain-containing protein", "db_xrefs": [ "RefSeq:WP_013526740.1", "SO:0001217", "UniParc:UPI0001A8C125", "UniRef:UniRef100_C4XEF8", "UniRef:UniRef50_C4XEF8", "UniRef:UniRef90_C4XEF8" ], "nt": "ATGAAAAAATATTACATTGCCTATGGATCTAATTTAAATTCGGGACATATGGAAAACTTATGTCCCTCAGCAAAATTTTTAGGTAAAGTTATTTTAAATAATTTTTCTTTAAGTTTTGAGGGAGAAAAAGATCGTTGTTATTTAAATATCAGAAAAGTAAAAGACAAAAATATTGAAGTTGGAGTTTTTGAATTAAATGATAAAGATATTTTTAAATTAGATGAATATGAAGATTATCCTTTTTTATATAAAAAAGAAGATATTGAAATTGAATTAAATAGCAAAAAAATTAAAGCTTTTTATTATTATATGGATTGCGGATATAGTCCTTGCAAACCAAATAAAGAATATGTTGAAATGTGCTTAAAAGGATACCAAGACTGCAGTATTAAAAAAGAACAACTTAGAGAGATTTTATACAAGTAA", "aa": "MKKYYIAYGSNLNSGHMENLCPSAKFLGKVILNNFSLSFEGEKDRCYLNIRKVKDKNIEVGVFELNDKDIFKLDEYEDYPFLYKKEDIEIELNSKKIKAFYYYMDCGYSPCKPNKEYVEMCLKGYQDCSIKKEQLREILYK", "aa_hexdigest": "5208f99e708b19b80dd149db43d84edf", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C125", "ncbi_nrp_id": "WP_013526740.1", "uniref100_id": "UniRef100_C4XEF8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C125", "RefSeq:WP_013526740.1", "UniRef:UniRef100_C4XEF8" ] }, "ips": { "uniref100_id": "UniRef100_C4XEF8", "uniref90_id": "UniRef90_C4XEF8" }, "psc": { "uniref90_id": "UniRef90_C4XEF8", "product": "Gamma-glutamylcyclotransferase AIG2-like domain-containing protein", "uniref50_id": "UniRef50_C4XEF8" }, "pscc": { "uniref50_id": "UniRef50_C4XEF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEF8" ], "product": "Gamma-glutamylcyclotransferase AIG2-like domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_547", "locus": "GCA000209735_00521" }, { "type": "cds", "sequence": "contig_1", "start": 282601, "stop": 282864, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5A111", "UniRef:UniRef100_A0AB32XB79", "UniRef:UniRef50_A0AB32XB79", "UniRef:UniRef90_A0AB32XB79" ], "nt": "ATGATTAACAAGGAATATAAGCCTATATTCAAGGTTACAAACAAAATAATTGAATTAATTGGCAAGATTAAAGAGAAATTGTCAAGCGTTTTTTTATGCAAAGATTTTTTGATTTTGAATAGAAAAATAAAAATTAAATCTATTTATTCAAGTGTGAAAATTGAAGGTAATCCCTATGATTTTCAAACTTTTTATAAAGTCATTAATAATGAAGAAAAAGAACAAAAAGATAAATATAAATTGGAATTTAAAAAGGTGCTGTAA", "aa": "MINKEYKPIFKVTNKIIELIGKIKEKLSSVFLCKDFLILNRKIKIKSIYSSVKIEGNPYDFQTFYKVINNEEKEQKDKYKLEFKKVL", "aa_hexdigest": "519bc18f3343baa767c8cdbcd657e2a3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F5A111", "uniref100_id": "UniRef100_A0AB32XB79", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5A111", "UniRef:UniRef100_A0AB32XB79" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XB79", "uniref90_id": "UniRef90_A0AB32XB79" }, "psc": { "uniref90_id": "UniRef90_A0AB32XB79", "uniref50_id": "UniRef50_A0AB32XB79" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XB79", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XB79" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10453.347299999992, "isoelectric_point": 9.630948066711426 }, "id": "OAIKOHHJFB_548", "locus": "GCA000209735_00522" }, { "type": "cds", "sequence": "contig_1", "start": 282959, "stop": 283165, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEU7", "UniRef:UniRef90_C4XEU7" ], "nt": "ATGTCTTATACAGGAAATTTTGATACTTTTTCAACATTCTTTCAAAACTATGGATTAAATTATGACAAGTTAGAATCAGAAAAAAAACAAATAATAATTAATAAAAAATTATTACCAGAGAAAACGGCAAATTCTAAAATTTCTGAAACTAAAACTGAATCTTTGTTCAAAAAGATTAGTTCAAAATCTTTATTTGAAAAAATGTGA", "aa": "MSYTGNFDTFSTFFQNYGLNYDKLESEKKQIIINKKLLPEKTANSKISETKTESLFKKISSKSLFEKM", "aa_hexdigest": "d14ed2add281c7a34f2d13d149b41d1e", "start_type": "ATG", "rbs_motif": "AGGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 282959, "stop": 283268, "observations": { "insertion": [ 283238 ], "mutation": [ 283163 ], "stop": [ 283163 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEU7", "query_cov": 1.0, "subject_cov": 0.6666666666666666, "identity": 1.0, "score": 126.0, "evalue": 1.49e-36, "gene_start": 1, "gene_end": 68, "reference_start": 1, "reference_end": 68, "reference_sequence": "MSYTGNFDTFSTFFQNYGLNYDKLESEKKQIIINKKLLPEKTANSKISETKTESLFKKISSKSLFEKMWKSKKYLNFADKALIFLSTLFKMKIQYQKEFLMN" }, "paralog": false, "identity": 0.9901960784313726, "score": 192.0, "evalue": 5.49e-65, "description": "Internal stop codon at 283163. Frameshift due to insertion around 283238. Nonsense mutation around 283163.", "psc": { "uniref90_id": "UniRef90_C4XEU7", "uniref50_id": "UniRef50_C4XEU7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEU7", "UniRef:UniRef50_C4XEU7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEU7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEU7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7940.015899999999, "isoelectric_point": 9.398668479919433 }, "id": "OAIKOHHJFB_549", "locus": "GCA000209735_00523" }, { "type": "cds", "sequence": "contig_1", "start": 283229, "stop": 283453, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATAACAATATCAAAAAGAATTTTTGATGAATTAGTATCAATGAATTCAAAGGTATGTTTTTATGTTTATATTTTTTTGATATATTCAGGAGCTAGAAGAAAATTTGTAGAATTAACAGGATTAATGAAATATGGTTTTGCTAGAAGTTCATTATTTTTTGCATACAAAAAATTGCTTAAAAGTAAATTGATTAAAACCATCAACAAAAACGATTCTTGA", "aa": "MKITISKRIFDELVSMNSKVCFYVYIFLIYSGARRKFVELTGLMKYGFARSSLFFAYKKLLKSKLIKTINKNDS", "aa_hexdigest": "ee1a46cfc99fa70b8a20f44b791b3aad", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8704.383199999993, "isoelectric_point": 10.111495399475096 }, "id": "OAIKOHHJFB_550", "locus": "GCA000209735_00524" }, { "type": "cds", "sequence": "contig_1", "start": 283565, "stop": 283837, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATTTATACCTAAATTTTTGTTTTTAACATATAAGTCTAAAAAAGTGTCTAAAAACAAATATGAAGTAAATACTGATAATAAAGTTTTAATTAATAATAAGTTTTTAAATTTGTCAAAAAATCAAGGCTATGTCTTTTTTAAATCAGTTGTTGCTGTTCTTGGAATTGATGTAATTAGCTCAGATTTACAAGCTTTCGAATATGTTAGAGAATATGACCTTAATTCACATTCTTTTAAATGCTTTCGTTGGCTAGTTATGAGAATATAA", "aa": "MKFIPKFLFLTYKSKKVSKNKYEVNTDNKVLINNKFLNLSKNQGYVFFKSVVAVLGIDVISSDLQAFEYVREYDLNSHSFKCFRWLVMRI", "aa_hexdigest": "a6cf9241f23083862b43c93386ae60e8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10698.441299999997, "isoelectric_point": 9.751826286315914 }, "id": "OAIKOHHJFB_551", "locus": "GCA000209735_00525" }, { "type": "cds", "sequence": "contig_1", "start": 284676, "stop": 285176, "strand": "+", "frame": 3, "gene": null, "product": "HpyAIV family type II restriction enzyme", "db_xrefs": [ "EC:3.1.21.4", "GO:0009036", "GO:0009307", "SO:0001217", "UniRef:UniRef50_C4XE10", "UniRef:UniRef90_C4XE10" ], "nt": "ATGGAATATAGTCAATTTGAAACAGAACTTAAAAGAGCCTTAACAAATGATGCCACATCAAAACTTATAAAAGAAATAATCAATAAACCTTTTAATTTTGTTTCACCAACCAACCCATTTGATGTTAAAACTAAAATTAAACAATCATTTTTGAAGCATCAAGAAAGAATTTATGAAAAATTTATTCAATATTGTGCTAAACGTTTAATTACTCAATATGGTTTTAGTTTACAAAGAAGTAATTTTAGATACAATTTGCCTCTTAAAGAGGGACAACACTTAGGAGACATTCCTGAACAAGTAATAATCAATGCATCTTTCATTTTTAGATTGAATAATAATTCAACAAAAGATGAACCAACAGATATGTTTGTTGTTTATTTAAGAAAGAGAGATTGCTATTCACATAAAGCAATGAAAAAAACTTGTGAGAATATCATTAAACAATTAGAAGCCTTAAAAATTATTTACCCAAAAATGAATATTAAGGGTGCTTTATGA", "aa": "MEYSQFETELKRALTNDATSKLIKEIINKPFNFVSPTNPFDVKTKIKQSFLKHQERIYEKFIQYCAKRLITQYGFSLQRSNFRYNLPLKEGQHLGDIPEQVIINASFIFRLNNNSTKDEPTDMFVVYLRKRDCYSHKAMKKTCENIIKQLEALKIIYPKMNIKGAL", "aa_hexdigest": "db6b55899c8f181b6b7105e638679bda", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 284676, "stop": 285479, "observations": { "mutation": [ 285174, 285297, 285423 ], "stop": [ 285174, 285297, 285423 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XE10", "query_cov": 1.0, "subject_cov": 0.5763888888888888, "identity": 0.9520000000000001, "score": 316.0, "evalue": 3.11e-107, "gene_start": 1, "gene_end": 166, "reference_start": 1, "reference_end": 166, "reference_sequence": "MEYSQFEAELKSALTNDPTLKLLKEIINKPFNFVSPTNPFDVKTKIKQSFLKHQERIYDKFIQYCALSLITQYGFSLQRSNFRYNLPLKEGQHLGDIPEQVIINASFIFRLNNNSTKDEPTDMFVVYLRKRDCYSHKAMKKTCENIIKQLEALKIIYPKMNIKGALWFLDNDYIKNIRLFNNFYYEQVDLRPYYGADFFEILDKKEDWLKIESFIKKFKENNQNYFSKMPDLDQSDEALIALLYLEKSEWTKLNSNKEIYRNIKENIFNLNDKNSNLFKAIAKRNKSY" }, "paralog": false, "identity": 0.9550561797752809, "score": 510.0, "evalue": 3.62e-186, "description": "Internal stop codon at 285174, 285297, 285423. Nonsense mutation around 285174, 285297, 285423.", "psc": { "uniref90_id": "UniRef90_C4XE10", "product": "HpyAIV family type II restriction enzyme", "ec_ids": [ "3.1.21.4" ], "uniref50_id": "UniRef50_C4XE10", "go_ids": [ "GO:0009036", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XE10", "EC:3.1.21.4", "UniRef:UniRef50_C4XE10", "GO:0009036", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE10", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE10" ] } }, "id": "OAIKOHHJFB_552", "locus": "GCA000209735_00526" }, { "type": "cds", "sequence": "contig_1", "start": 286283, "stop": 286753, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013526745.1", "SO:0001217", "UniParc:UPI0001F5A115", "UniRef:UniRef100_C4XEG3", "UniRef:UniRef50_C4XEG3", "UniRef:UniRef90_C4XEG3" ], "nt": "ATGGAATCAATTAGCAAATTAATAGATTCAAAAACAATAATCAATTTTGACATTGCAAAGTTGCAAAGTATTAATAAATATTTTGGATTTGGTATATCTGATATTAATTTATTAAAAATCAATCAGGTTTTATTTGATTATGAATTATTAAAAACTCTTAGATTAGAAAATAATTTACTTCAACTAAATATATATTATGAATTACTAAAAGCATTTAAATTGAATAAAAGTAATGATTTTAAAAAGAATTTCAAATTCCAAAGAATTAAAAATGAATTAATGGAAAAAATTAAAAAAATTTTTAAATTACTTGGAAATAATCAGATTGAAGAAAAAGAATATCAGGATTACATTTATGAAAAATATTGTTATGGCTTCAAAAAATCTCCTGAAAATATTTCATTCAAAATACAAAGAATTTTTATAAATATATTGTCTGAATATGAAAACTACTTAGATAGTTTTGATTAG", "aa": "MESISKLIDSKTIINFDIAKLQSINKYFGFGISDINLLKINQVLFDYELLKTLRLENNLLQLNIYYELLKAFKLNKSNDFKKNFKFQRIKNELMEKIKKIFKLLGNNQIEEKEYQDYIYEKYCYGFKKSPENISFKIQRIFINILSEYENYLDSFD", "aa_hexdigest": "915f0a1a2ff5d36ed2d11f97c42870a7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F5A115", "ncbi_nrp_id": "WP_013526745.1", "uniref100_id": "UniRef100_C4XEG3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5A115", "RefSeq:WP_013526745.1", "UniRef:UniRef100_C4XEG3" ] }, "ips": { "uniref100_id": "UniRef100_C4XEG3", "uniref90_id": "UniRef90_C4XEG3" }, "psc": { "uniref90_id": "UniRef90_C4XEG3", "uniref50_id": "UniRef50_C4XEG3" }, "pscc": { "uniref50_id": "UniRef50_C4XEG3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEG3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18867.681699999994, "isoelectric_point": 8.92366542816162 }, "id": "OAIKOHHJFB_553", "locus": "GCA000209735_00527" }, { "type": "cds", "sequence": "contig_1", "start": 286767, "stop": 286931, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTATATAGAAAAGGAAAAATAGTTTACAAAGATGGAAAAAATCTAATTTTTGAATCAGATAATGTTGGATACTATGTTACGTTTCCAGATAACAAAAGGATTAAATTAAATCAAGAATTAAAAATGTGTTTATATGAAATTAAAAATGATTTTACTTGA", "aa": "MVLYRKGKIVYKDGKNLIFESDNVGYYVTFPDNKRIKLNQELKMCLYEIKNDFT", "aa_hexdigest": "c8dc609ca0a49ee72ca57b660e6dd3fc", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6497.5178, "isoelectric_point": 9.09843921661377 }, "id": "OAIKOHHJFB_554", "locus": "GCA000209735_00528" }, { "type": "cds", "sequence": "contig_1", "start": 286883, "stop": 287074, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCCATCTCTAATTAAATTTATAGCTTTATCAAATCCAACATTTAAAACATTAAATGCTACTCTAGGACCAATGCCATTTAAATTAAGTAAATCAGCAAATAATTTTCTTTCTTCTAATTCTTTAAAACCATATAAAGATCAAGTAAAATCATTTTTAATTTCATATAAACACATTTTTAATTCTTGA", "aa": "MLPSLIKFIALSNPTFKTLNATLGPMPFKLSKSANNFLSSNSLKPYKDQVKSFLISYKHIFNS", "aa_hexdigest": "2f86f56e58c2d4a6a30a4f5d75ffa7b6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7080.271900000002, "isoelectric_point": 10.176221656799317 }, "id": "OAIKOHHJFB_555", "locus": "GCA000209735_00529" }, { "type": "cds", "sequence": "contig_1", "start": 287186, "stop": 287368, "strand": "+", "frame": 2, "gene": "ruvA", "product": "Holliday junction DNA helicase RuvA C-terminal domain-containing protein", "db_xrefs": [ "RefSeq:WP_013526656.1", "SO:0001217", "UniParc:UPI0001A8C12C", "UniRef:UniRef100_C4XEG5", "UniRef:UniRef50_C4XEG5", "UniRef:UniRef90_C4XEG5" ], "nt": "ATGTTTGAAAATTATGTAACAACATATAAATCATCAAAAGAAATCAATGAAACTTTGAAAATGTTAGGTTTTAAAGACTCTCTTATTGATGATGTAGTAAGAAAAATCAATTTAAATCAAGACATAGAATTTTCACTAGAAGAAGCAATTAAATTAATGTCTGATAAACCAAAAACACTATAA", "aa": "MFENYVTTYKSSKEINETLKMLGFKDSLIDDVVRKINLNQDIEFSLEEAIKLMSDKPKTL", "aa_hexdigest": "c6bdaa9e4aa39c8bf37f7321449d566e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C12C", "ncbi_nrp_id": "WP_013526656.1", "uniref100_id": "UniRef100_C4XEG5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C12C", "RefSeq:WP_013526656.1", "UniRef:UniRef100_C4XEG5" ] }, "ips": { "uniref100_id": "UniRef100_C4XEG5", "uniref90_id": "UniRef90_C4XEG5" }, "psc": { "uniref90_id": "UniRef90_C4XEG5", "product": "Holliday junction DNA helicase RuvA C-terminal domain-containing protein", "uniref50_id": "UniRef50_C4XEG5" }, "pscc": { "uniref50_id": "UniRef50_C4XEG5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEG5" ], "product": "Holliday junction DNA helicase RuvA C-terminal domain-containing protein" }, "genes": [ "ruvA" ], "id": "OAIKOHHJFB_556", "locus": "GCA000209735_00530" }, { "type": "cds", "sequence": "contig_1", "start": 287387, "stop": 287827, "strand": "+", "frame": 2, "gene": "ruvB", "product": "Holliday junction DNA helicase RuvB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32EA0", "UniRef:UniRef90_UPI0001E32EA0" ], "nt": "ATGTATAATTTAACACCAATAAATTTTAAAGAGTTTAGAGGACAAAAAGATTTAATAAAATCATTGATTATATTGCTTCAAGAAAGTAAAAAAATTCCAAATATGATTTTTGTTGCTCCAAAGGGATATGGCAAATATTCTTTAGCGAATATAATTTCTCAATTTAAGGATAGAAGTTTGCAACCTATAACTAGTAATAATATTGTTGATGAATTTGAACTGTTAAACATACTAGCAAATTTAAAAAATAATGCAATCTTATTCATTGAAGATATTAATAAATTAAAACCAGAGCTCAAAAAATTATTATTTGATATTTATAACTCGATAAATCTAGATAAAGTTGAAAAAATATGTTATTCAAACAAGCGTTTTAAGATTAAAAATTTTTCGATTATAGGGACAATAAATTCTTCAAATCAAATTGATTCCTCAAAATGA", "aa": "MYNLTPINFKEFRGQKDLIKSLIILLQESKKIPNMIFVAPKGYGKYSLANIISQFKDRSLQPITSNNIVDEFELLNILANLKNNAILFIEDINKLKPELKKLLFDIYNSINLDKVEKICYSNKRFKIKNFSIIGTINSSNQIDSSK", "aa_hexdigest": "f2a1161e604ea609c72117339484b6f6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0001E32EA0", "query_cov": 1.0, "subject_cov": 0.948051948051948, "identity": 0.993, "score": 279.0, "evalue": 5.88e-95, "valid": true, "product": "Holliday junction DNA helicase RuvB", "uniref50_id": "UniRef50_UPI0001E32EA0" }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32EA0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32EA0" ], "product": "Holliday junction DNA helicase RuvB" }, "genes": [ "ruvB" ], "id": "OAIKOHHJFB_557", "locus": "GCA000209735_00531" }, { "type": "cds", "sequence": "contig_1", "start": 288078, "stop": 288773, "strand": "+", "frame": 3, "gene": null, "product": "DNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32E9F", "UniRef:UniRef90_UPI0001E32E9F" ], "nt": "ATGAGAAAATCAATTAAGTTTTTTAAAGATATAGATAAAAAAATGTTTATTTGTGAAGCAAATTTAAACAAGGAATACAGTCCTAATAGTTTTAATTCAATATCTTCTTATTTAGCTATGTTTCCTGTTGAATTACCGTTATATTTTATAAAAAAATATACCAAGGAAAATGATACAGTTATGGATAACTTTTCAGGAAGAGGCACAACAGCTTATGCATCAAGAAAATTAAATAGAAAATTTGTAGGTAATGATTTAAACCCTTATGCATTTGTATTGTCAAAATCCAAACTTATAAATATATCAAATATAGAAAAGATTGAAAAACGAATAAAAGAATTAAAAAACAAGTATTTGCTTTTGCCAAGGTCTATAAATATAACGAGTGAAAAATATAATGATTTAAAAGTTTTTTATAGTGATTTTACACTTAAACAACTAATTTTTCTAAAAAATGAAATTGGCATTAATTGGAAAAATAACAAAGATATTGATAATTTGATACTTGCATTATCATTAGGACTTATGCACGGTCCAATGAAAAAAGATGGTACAACAATTTATTTTTCGTTAAATATGCCAAATACTATTTCAATGTCTACAAATTATGTTAAAAAATATTCTGAAATAAATAAATTAACTAAACCTAATGTTGACATATTCGATAATTTAATTAAAAGATTAAAAATTAAATGA", "aa": "MRKSIKFFKDIDKKMFICEANLNKEYSPNSFNSISSYLAMFPVELPLYFIKKYTKENDTVMDNFSGRGTTAYASRKLNRKFVGNDLNPYAFVLSKSKLINISNIEKIEKRIKELKNKYLLLPRSINITSEKYNDLKVFYSDFTLKQLIFLKNEIGINWKNNKDIDNLILALSLGLMHGPMKKDGTTIYFSLNMPNTISMSTNYVKKYSEINKLTKPNVDIFDNLIKRLKIK", "aa_hexdigest": "9122dc5204b70e2613022a967e5f69c7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0001E32E9F", "query_cov": 0.9653679653679653, "subject_cov": 1.0, "identity": 0.991, "score": 439.0, "evalue": 1.61e-155, "valid": true, "product": "DNA methyltransferase", "uniref50_id": "UniRef50_UPI0001E32E9F" }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32E9F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32E9F" ], "product": "DNA methyltransferase" }, "genes": [], "id": "OAIKOHHJFB_558", "locus": "GCA000209735_00532" }, { "type": "cds", "sequence": "contig_1", "start": 289294, "stop": 289425, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACACACCAAATAATAAAATTAATATGTTATCAACCATACCATTAAAAATTAAAGGTCGAGCAATTATTGAAGATTATCTAAAATTAAAAGTTAATTCTATTCTATTTATGGCAGAAGATGATGAATGA", "aa": "MNTPNNKINMLSTIPLKIKGRAIIEDYLKLKVNSILFMAEDDE", "aa_hexdigest": "1bc3e1363e697e2abdb23ef609e7e097", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4935.7792, "isoelectric_point": 6.142328453063963 }, "id": "OAIKOHHJFB_559", "locus": "GCA000209735_00533" }, { "type": "cds", "sequence": "contig_1", "start": 289426, "stop": 289761, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEG8", "UniRef:UniRef90_C4XEG8" ], "nt": "TTGTTTTTTGTTGCATGTTATCCAAAAAATGCTAAGGTAAAAAATGATAAAGAAAACTTAATTGACATAAGAAATAATCAAGTAAATTCAAAAGATGAATTAGATGATGTAAAAGAAATTTCATCTTATATTGACCTAAGTCATATTTTTAAGATACATGTAAAAAATTTAAAAGATTTTGTTAAATGTGCAACAGATGAAAGTTATCCATTTTTGAATTTACAAAAGCAACTTGAATATGTTGAAAAAATAACTAGTTTAACAAATGGCGAAAGATCAAAAGCGCCAAAATTAGTAAGAATAAACAAAAAAGGAGACAAGCTTATTGAAGCATAA", "aa": "MFFVACYPKNAKVKNDKENLIDIRNNQVNSKDELDDVKEISSYIDLSHIFKIHVKNLKDFVKCATDESYPFLNLQKQLEYVEKITSLTNGERSKAPKLVRINKKGDKLIEA", "aa_hexdigest": "05d6c4774ad92cc4040ff46cab4c8f18", "start_type": "TTG", "rbs_motif": "AGxAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 289264, "stop": 289761, "observations": { "mutation": [ 289423 ], "stop": [ 289423 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEG8", "query_cov": 1.0, "subject_cov": 0.6727272727272727, "identity": 0.991, "score": 216.0, "evalue": 2.87e-70, "gene_start": 1, "gene_end": 111, "reference_start": 55, "reference_end": 165, "reference_sequence": "MNNYKKQGENMNTPNNKINMLSTIPLKIKGRAIIEDYLKLKVNSILFMAEDDEWLFFVACYPKNAKVKNDKENLIDIRNNQVNSKDELDDVKEISSYIDLSHIFKIHVKNLKDFVKCATDESYPFLNLQKQLEYVEKITSLTNGERSKAPKLVRINKKGDKLIEA" }, "paralog": false, "identity": 0.9878787878787879, "score": 325.0, "evalue": 1.21e-115, "description": "Internal stop codon at 289423. Nonsense mutation around 289423.", "psc": { "uniref90_id": "UniRef90_C4XEG8", "uniref50_id": "UniRef50_C4XEG8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEG8", "UniRef:UniRef50_C4XEG8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEG8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEG8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 12876.668799999981, "isoelectric_point": 8.799241447448733 }, "id": "OAIKOHHJFB_560", "locus": "GCA000209735_00534" }, { "type": "cds", "sequence": "contig_1", "start": 289781, "stop": 290140, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATACAATAAATTCAAAGAAAAATTATTAGATGTTTTTAAATCAAGAAGAGTCAAAAGTTTAATTGATTTGATTATGAGAAAACCAAATAAATTTTTATCTAAATCTAAACCTTATAATAGTAAAGAAATTATAAATTTAGAATATTATAGACTTCAAAATGTTGAGTACTTCAAGTTTTTAAAAGAAGAAATTAAAAATGTGTTAAAAGAAAACAAATATACTATTGAAAAAACAACTGCCAAAGATATAAATACAATTTTAAAAGGCAAGGAATATAAAAACATAGAAGTGCCAATTAACTTTGATTTTATTTTTAGAAAAAATGGTGATACTTCTTGCTTAACTGTGTTATGA", "aa": "MTYNKFKEKLLDVFKSRRVKSLIDLIMRKPNKFLSKSKPYNSKEIINLEYYRLQNVEYFKFLKEEIKNVLKENKYTIEKTTAKDINTILKGKEYKNIEVPINFDFIFRKNGDTSCLTVL", "aa_hexdigest": "960afa9397d38dc95b18e550185890cb", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14329.662599999978, "isoelectric_point": 9.686004066467284 }, "id": "OAIKOHHJFB_561", "locus": "GCA000209735_00535" }, { "type": "cds", "sequence": "contig_1", "start": 290414, "stop": 290632, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATATTCAACTAATTTCAAAAAAGAATTTAAAAACTTATTTATTAAAGGAATTGATTTTGATAAAGATAAAAATGTTTTTGAAATCTTTAAAAAAATAAACAAAATCAAATTTTACAATTTCCTTTTTAAAGATGATTCTAAATTAGAACAAATTAGACAATATATATTTTCAAATGATAAAAACTCCAATAGATTAAAACTTTTAAGATATTGA", "aa": "MTYSTNFKKEFKNLFIKGIDFDKDKNVFEIFKKINKIKFYNFLFKDDSKLEQIRQYIFSNDKNSNRLKLLRY", "aa_hexdigest": "2a864b125d5f55f005552adb089c6bbc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8961.30509999999, "isoelectric_point": 9.801918220520019 }, "id": "OAIKOHHJFB_562", "locus": "GCA000209735_00536" }, { "type": "cds", "sequence": "contig_1", "start": 290667, "stop": 291113, "strand": "+", "frame": 3, "gene": null, "product": "DUF4116 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH0", "UniRef:UniRef90_C4XEH0" ], "nt": "ATGAAAAAATTTAGATATTATACAGACAGAGAAAGTTTACTAAAAGCTGTCACAAATGATGGAATGAGAATTGAGTATTGTCTTCCAGAATTTAAAAATGATAAAGAAATAGTGTTAAAAGCTGTAAAAAATAATGGAAGAGCTTATTACTATTCATCTCAAGAATTACAAAAAGATGATGATGTTATAAAAGCTTCTTTTATTCAATCAGGAGATACAAAAATTTTTGATAATTTTTCTGATAAATGTAAAAACAATAAAAAAATGATTTTAAAATTGATAGAAAGAAATCCATATATTTTCAGGTATTTACCTGATTCACTTAAAAAAGATTTAAATGTCTGTTTTAAAGCTTTTAATATTCACCCGGCAGTTATTAAATTAGCACCAGATGAAATTTTAAAGAATCAAAGCTTAATTTTAAGATTAATCAAGAACAAAGAATGA", "aa": "MKKFRYYTDRESLLKAVTNDGMRIEYCLPEFKNDKEIVLKAVKNNGRAYYYSSQELQKDDDVIKASFIQSGDTKIFDNFSDKCKNNKKMILKLIERNPYIFRYLPDSLKKDLNVCFKAFNIHPAVIKLAPDEILKNQSLILRLIKNKE", "aa_hexdigest": "4164854a7130acf16c96c160bbf5ab07", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 290628, "stop": 291257, "observations": { "mutation": [ 291111 ], "stop": [ 291111 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEH0", "query_cov": 1.0, "subject_cov": 0.7081339712918661, "identity": 0.986, "score": 158.0, "evalue": 3.12e-46, "gene_start": 1, "gene_end": 148, "reference_start": 14, "reference_end": 161, "reference_sequence": "MEKKILTKEERKKMKKFRYYTDRESLLKAVTNDGMRIEYCLPEFKNDKEIVLKAVKNNGRAYYYSSQELQKDDDVIKASFIQSGDTKIFDNFSDKCKNNKKMILKLIERNPYIFRYLPDSLKKDLNVCFKAFNIHPAVIKLAPDEILKNQSLILRLIKNKEWILKYCGELNSNKKFMVKAVSVNEKCLKFVSEDLKNDKDFIFKISKNL" }, "paralog": false, "identity": 0.9904306220095693, "score": 272.0, "evalue": 8.95e-94, "description": "Internal stop codon at 291111. Nonsense mutation around 291111.", "psc": { "uniref90_id": "UniRef90_C4XEH0", "product": "DUF4116 domain-containing protein", "uniref50_id": "UniRef50_C4XEH0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEH0", "UniRef:UniRef50_C4XEH0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEH0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH0" ], "product": "DUF4116 domain-containing protein" } }, "id": "OAIKOHHJFB_563", "locus": "GCA000209735_00537" }, { "type": "cds", "sequence": "contig_1", "start": 291156, "stop": 291257, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13475.11" ], "nt": "ATGGTTAAAGCTGTAAGTGTAAATGAAAAATGCTTGAAATTTGTTAGTGAAGATTTAAAAAACGATAAAGATTTTATCTTTAAAATAAGCAAAAATCTATAA", "aa": "MVKAVSVNEKCLKFVSEDLKNDKDFIFKISKNL", "aa_hexdigest": "dc21e3d4ff9a112668b03ef0eb09110b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13475.11", "name": "DUF4116", "length": 27, "aa_cov": 0.8181818181818182, "hmm_cov": 1.0, "evalue": 4.094135739155096e-09, "score": 32.01211929321289, "start": 3, "stop": 29 } ], "seq_stats": { "molecular_weight": 3831.5015000000003, "isoelectric_point": 8.931595039367679 }, "id": "OAIKOHHJFB_564", "locus": "GCA000209735_00538" }, { "type": "cds", "sequence": "contig_1", "start": 291263, "stop": 291403, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAAAAAGATCAAAATTCTGAAGTTTTTTCAAAAGAAAATAATACAATCCCAAAACAAATTCCAAGCACAGTTTATACTTTAGAAAGCACTATACAAGTGCCAATAACAAACTTTACTAAAAAAAGAATTTTTTGA", "aa": "MQEKDQNSEVFSKENNTIPKQIPSTVYTLESTIQVPITNFTKKRIF", "aa_hexdigest": "cc5458cac4667834d74f24e26816b731", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5374.040500000001, "isoelectric_point": 8.099630546569824 }, "id": "OAIKOHHJFB_565", "locus": "GCA000209735_00539" }, { "type": "cds", "sequence": "contig_1", "start": 291751, "stop": 291999, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCAGAAATAACTTTTTTATCACTAAAACCTTCTGCATTTTCCTTTTTTATAGTTGGGTTTTTAGATGGTTTTGGTGGATTAATAGGAAAAGCAAACCAATTACAACTAGTTGCTGTTAAAGCAGTTACAATTGGTAATGTTATTGTTGGTAATATTGTCTTTAATCATATCTTTTTAGTTTTCATATTGCTCCTTATTTGCTATATAAATATTATACTTTATATTTATATTAAAATAAATCTTTAA", "aa": "MAEITFLSLKPSAFSFFIVGFLDGFGGLIGKANQLQLVAVKAVTIGNVIVGNIVFNHIFLVFILLLICYINIILYIYIKINL", "aa_hexdigest": "f50b57062a5a2a7a8f94084e8acefc52", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9064.948599999994, "isoelectric_point": 8.93223972320557 }, "id": "OAIKOHHJFB_566", "locus": "GCA000209735_00540" }, { "type": "cds", "sequence": "contig_1", "start": 292247, "stop": 292405, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATATTTTGAAAATAAAAAATTTGAAGTTTTAGACAACAAAGAAGAAGTAGAAAAAAACCATAAAAGTCCAACATATATTTATAAAAATTACATTGAATGTGTAGATGCTTTAGGATATTTTATGGAAAATTATAAAGATCCAGACTTAAAATGA", "aa": "MKYFENKKFEVLDNKEEVEKNHKSPTYIYKNYIECVDALGYFMENYKDPDLK", "aa_hexdigest": "84e4dd2b6bf174ecff57cd3cc33d7ffb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6403.183200000001, "isoelectric_point": 5.264279747009277 }, "id": "OAIKOHHJFB_567", "locus": "GCA000209735_00541" }, { "type": "cds", "sequence": "contig_1", "start": 292601, "stop": 292909, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACTCAAAACAAATTCAAGCACTAGATAAAATTTATCTAATGATGGAAGAAGCAACAAGAGCTTTTTATAGAGTAAAGCATTTTGAAAATTTATGTAAACCATACAACACAATAGGTAATCAAATCAACTTATATGTATCAATGATTAATTTATTTAAAAACTTGTATAACTTAAAAATTGATACTAATAAAACATTAAAAGAACAATTGCAAGATTTAAAACCTGAAGTTTTAAGAGCTTTAGAGAAATCAAAAAATGCATTAAATAAATTATTACCTATTATTGATTCTGGTTTTGGATCGTGA", "aa": "MNSKQIQALDKIYLMMEEATRAFYRVKHFENLCKPYNTIGNQINLYVSMINLFKNLYNLKIDTNKTLKEQLQDLKPEVLRALEKSKNALNKLLPIIDSGFGS", "aa_hexdigest": "1cdeb99ea6952a6abf34c664964b4a43", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11882.784499999983, "isoelectric_point": 9.380423927307131 }, "id": "OAIKOHHJFB_568", "locus": "GCA000209735_00542" }, { "type": "cds", "sequence": "contig_1", "start": 293356, "stop": 293733, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59BE8", "UniRef:UniRef100_A0AB32XAQ5", "UniRef:UniRef50_C4XEH2", "UniRef:UniRef90_C4XEH2" ], "nt": "TTGATAAAAAGCAAGAAAAATACAAGCACTATTTCTGATAGTGCATTTTTTGCTTTTGGAAAGGAAAAAATGAAAATAACATCTTTATATTATGCAAACAATAATCCAGGAATATCAGTATTAAAAAATACATCGACTTATGTACTTGATGAATTTGTAAAATACTCTGCTATTATTTTTGGAATTTTGTTTGCAATTGCATTTGTAACATCAATAATCACAATTGCTATGAATGCTGCATTTACTTCAAAAGGCAACAATACTCCTGCAAAGCTAAAAAAGGCACTAAATGGTTCTTTAATTTCTTGTGGTGTTCTTTTAGCATTATTAATTTTGGGGCCTGGACTATGTGGTTTAATTTCTAATTCATTGAAATAA", "aa": "MIKSKKNTSTISDSAFFAFGKEKMKITSLYYANNNPGISVLKNTSTYVLDEFVKYSAIIFGILFAIAFVTSIITIAMNAAFTSKGNNTPAKLKKALNGSLISCGVLLALLILGPGLCGLISNSLK", "aa_hexdigest": "541bd4002d390de57c1f8e6291c84a4a", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59BE8", "uniref100_id": "UniRef100_A0AB32XAQ5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59BE8", "UniRef:UniRef100_A0AB32XAQ5" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XAQ5", "uniref90_id": "UniRef90_C4XEH2" }, "psc": { "uniref90_id": "UniRef90_C4XEH2", "uniref50_id": "UniRef50_C4XEH2" }, "pscc": { "uniref50_id": "UniRef50_C4XEH2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH2" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13307.625799999976, "isoelectric_point": 9.769168281555174 }, "id": "OAIKOHHJFB_569", "locus": "GCA000209735_00543" }, { "type": "cds", "sequence": "contig_1", "start": 293874, "stop": 294110, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTTGGTAAACCACTTTCTAATATTAATAGTTATGAAGATTTAAAGGTATTGCCTGGTGCATTTATTGGCATAGCTATATTTAGCATTTGTTTATTTTTTATAATCTTAGCAATTAGTGCTTTTAAGCTTTCTTATAATAAAAGTAAAGAAAGCTTTCAATCATTTAAAGCAAGTTTGAAAGCTATAATACCTAATTTAGTTTTTATAATAGTAATACCAATTTTAATTTGA", "aa": "MIFGKPLSNINSYEDLKVLPGAFIGIAIFSICLFFIILAISAFKLSYNKSKESFQSFKASLKAIIPNLVFIIVIPILI", "aa_hexdigest": "c4a50977911cf7020c43ced63351ec3e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8654.436699999991, "isoelectric_point": 9.519095420837402 }, "id": "OAIKOHHJFB_570", "locus": "GCA000209735_00544" }, { "type": "cds", "sequence": "contig_1", "start": 294264, "stop": 294467, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGATGAGTTGAGTCTATTAAATTCAAATATGGGGAGCTTATTTTTAAATAGCTTCATAGCTATTTTTGCTTTATATATTGTTGGCAATATAACAATCAATATAAGTATCAATGTTTATCAAGCTTTTTTCTTATTTATTCAAGCTCCTTTAATTAGTGTTGTTTCTATTGAAGATGGTGGTAAAAAATTAAAAGTTTGA", "aa": "MKDELSLLNSNMGSLFLNSFIAIFALYIVGNITINISINVYQAFFLFIQAPLISVVSIEDGGKKLKV", "aa_hexdigest": "bcb457581ccf759ebf931c004962c8ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7397.688899999997, "isoelectric_point": 5.970674705505371 }, "id": "OAIKOHHJFB_571", "locus": "GCA000209735_00545" }, { "type": "cds", "sequence": "contig_1", "start": 294489, "stop": 294950, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGCAATTATGATTTTGAATTTATCAATTTCGTTTATGGTCTTATTTTTTAGTGGCGCATCAAAAATCATTAAGCCTATGTCAGATGCAACAGGTATTGATAGTGGCATTTGTGCCTTGATTGTTTACACAGGTGCATTAATTACTTCATTATCATTAAATAAAGTTATGTTTGGCATATTCGGAGTTGAATATACAAGTTATCTAGGGAAATTTACCAAAAAATTAGCTGCAAAAGCAACAAAAAAAATTTCAAAACGTAAATCTAATTCAAAGAATAATTCATCTTCAGAACCTGAAACCACTTCGCAAACAGATTATCATCCTACAGGGAACGCAAAAAATGCTGAGTTGATTATAATAATGCAAAATAATAATCAAACAAAAAAACCAGAAACCACTCCACAGACTATAGGTGCCGCAAAAAAAGCTAAAAAACCCGAAACTCTAAATGACTGA", "aa": "MLAIMILNLSISFMVLFFSGASKIIKPMSDATGIDSGICALIVYTGALITSLSLNKVMFGIFGVEYTSYLGKFTKKLAAKATKKISKRKSNSKNNSSSEPETTSQTDYHPTGNAKNAELIIIMQNNNQTKKPETTPQTIGAAKKAKKPETLND", "aa_hexdigest": "f013f8d8f6533028ea4dd7abb837224c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16557.087599999977, "isoelectric_point": 9.739512825012206 }, "id": "OAIKOHHJFB_572", "locus": "GCA000209735_00546" }, { "type": "cds", "sequence": "contig_1", "start": 294981, "stop": 295118, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCAATTGTCTAAAACTCTTCGAGGTGAAAAAGACCTTAAAAAAACATCCGAAATAAGCAAAGAATTAAGTGAGTTAATTGAAAATGAATCATCCATTAAAAGAATAATAACAAGCATGGAAAGGAAAAAATAA", "aa": "MDQLSKTLRGEKDLKKTSEISKELSELIENESSIKRIITSMERKK", "aa_hexdigest": "90459cbe5d27ff28c389422274ab21d9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5252.027499999999, "isoelectric_point": 9.156267356872558 }, "id": "OAIKOHHJFB_573", "locus": "GCA000209735_00547" }, { "type": "cds", "sequence": "contig_1", "start": 295277, "stop": 295723, "strand": "+", "frame": 2, "gene": null, "product": "DUF304 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH4", "UniRef:UniRef90_C4XEH4" ], "nt": "GTGGCTTTTTGTTCTTTAATTTGGTTAATTATTCCAAGCAAAAAACATAACTGTAGATTATATGTTTTAATACTAAGAATATTAAAATTTAAATCTTCAGTAAAAAAATATAAATTAAACAGTTCAAATGACACAAAACTTTTAATACCATTTAAAAGAATAATTGACAATAAATATGTTGAAACATTTTCATCAGTAGGAAAAGGATTATTTACAGTTTTTAAGTTTTATGGTAATAATATTTATTCTAATAATAGGGATGATAAGGTGGCGTTTTTTTCTCAACTAATTAATGTTTTAAATTCAATTGAAACAAAATTCACTTTTGTAAAAAAAACTTCTTCAGTTAACATTGATGACAATATTAAAATTAATGAAAATCTCTATAAAACAATTGATTTGGTAAAAACCAATGATGTTGTAAAGAAACATTTCATTTATAGTTAA", "aa": "MAFCSLIWLIIPSKKHNCRLYVLILRILKFKSSVKKYKLNSSNDTKLLIPFKRIIDNKYVETFSSVGKGLFTVFKFYGNNIYSNNRDDKVAFFSQLINVLNSIETKFTFVKKTSSVNIDDNIKINENLYKTIDLVKTNDVVKKHFIYS", "aa_hexdigest": "2bebc0e5b3a7cf54880aa9bcbd1b07b5", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 295121, "stop": 295723, "observations": { "mutation": [ 295163, 295253 ], "stop": [ 295163, 295253 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEH4", "query_cov": 1.0, "subject_cov": 0.74, "identity": 0.993, "score": 240.0, "evalue": 1.05e-78, "gene_start": 1, "gene_end": 148, "reference_start": 53, "reference_end": 200, "reference_sequence": "MNQNKKISSLKLNIWKNLNIYDAIIILGISGFSAALGFANGLPLWIKIIIFLVAFCSLIWLIIPSKKHNCRLYVLILRILKFKSSVKKYKLNSSNDTKLLIPFKRIIDNKYVETFSSVGKGLFTVFKFYGNNIYSNNRDDKVAFFSQLINVLNSIETKFTFVKKTSSVNIDDNIKINENLYKTIDLVKTNDVVKKHFIYS" }, "paralog": false, "identity": 0.99, "score": 337.0, "evalue": 1.53e-119, "description": "Internal stop codon at 295163, 295253. Nonsense mutation around 295163, 295253.", "psc": { "uniref90_id": "UniRef90_C4XEH4", "product": "DUF304 domain-containing protein", "uniref50_id": "UniRef50_C4XEH4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEH4", "UniRef:UniRef50_C4XEH4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEH4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH4" ], "product": "DUF304 domain-containing protein" } }, "id": "OAIKOHHJFB_574", "locus": "GCA000209735_00548" }, { "type": "cds", "sequence": "contig_1", "start": 295793, "stop": 296176, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAACTTAAACAATATGAAAATGAATTTATTAAGTCTTTTGAAACTACAAAAATACCACTTAATGTTGTTACTGGTATGGAATTAATAGACTTTGCTAATATGATTTTTAAAAAAAATATTTCAAAAAGTGACAAACAAAATTATTTTGTTGAAGAATTGGATACTGAATTAACAAAAGAACTTGATATGAAAATGCAAGGTCAAAAGTTAAATGATAAAGTAATTAAAAAACTTCAAAGTGACAAAGAATACAAATTAATTTCTTTATTAGCACCAGAAAGAGTTGAATTTAATTCCAAATACATAAAAGTTGATAATGAGTATTTATCATTTCAAACTTTAGTTGATTTTAACTCTTTACAAATTAATGAAGGTTGA", "aa": "MNKLKQYENEFIKSFETTKIPLNVVTGMELIDFANMIFKKNISKSDKQNYFVEELDTELTKELDMKMQGQKLNDKVIKKLQSDKEYKLISLLAPERVEFNSKYIKVDNEYLSFQTLVDFNSLQINEG", "aa_hexdigest": "54de155b356ae5aa8a0b75791cec2e64", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14989.076699999989, "isoelectric_point": 5.307647895812988 }, "id": "OAIKOHHJFB_575", "locus": "GCA000209735_00549" }, { "type": "cds", "sequence": "contig_1", "start": 296267, "stop": 296602, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCATTAAATGAAGCAAACGCTGAAACTAAAAGTAATTTTAAAATGCAAAAAATCAGTGTAGAACAAAAAGTTCTAGATGAGTTAGTAGATTTTATTAATGTTCAAAAAATGAATTTATTTGATTCTAATATCATTTTTGTAAATCAAAGTAAAACCTTAGAGGGAATAAAAGATTTAGAAACTAAAAATGATATAAATGCTAAAAAAGCATTTGCAAATGTTTATAAAATGCATTATTTACAAAAGCAAGCATTGCAAAATTCAATTTTTATCAAAGATGATTTTCTAAATAATGGAATTGAAATGAGTTCAAGAAATATAGTATATGGTTGA", "aa": "MSLNEANAETKSNFKMQKISVEQKVLDELVDFINVQKMNLFDSNIIFVNQSKTLEGIKDLETKNDINAKKAFANVYKMHYLQKQALQNSIFIKDDFLNNGIEMSSRNIVYG", "aa_hexdigest": "d468523640bb645f71595be49c8c4145", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12798.509799999985, "isoelectric_point": 6.572315406799317 }, "id": "OAIKOHHJFB_576", "locus": "GCA000209735_00550" }, { "type": "cds", "sequence": "contig_1", "start": 296738, "stop": 296869, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTGGTTCAGGTAAATCAGCAACTACCTCTAAAATAGCTTTGCATCAATTTGTAGTAGGAAATGAAGTTATTATCATTGACCCTCAAAGAGAATACAAAGACTTATGCAAAAAATTAGATGGTAACTGA", "aa": "MPGSGKSATTSKIALHQFVVGNEVIIIDPQREYKDLCKKLDGN", "aa_hexdigest": "fc61c848a8aa12fc080e164cc7532130", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4702.368800000001, "isoelectric_point": 7.907514762878418 }, "id": "OAIKOHHJFB_577", "locus": "GCA000209735_00551" }, { "type": "cds", "sequence": "contig_1", "start": 296876, "stop": 297709, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05707.18" ], "nt": "ATGGGTAAAGGATTAGAAACAAAAATTAATCCATTACAAATCAATTATGAAATTGAAAAAGATAATATAAAAGTCAGTGATGAATTATTAATTAGTAATCATACAAAAATGGTTAAAGAATTTTTCTCATTACTTTATCCAGATTTAGATGCAAGAGATTTAAGAGTAGCTAATTTTTTATTAAATGAATTTTATTTATCTAATAAGATAAAAAATATTCAAAAATTAAAAAACACAGATTTTCCAACAATGAAAGACTATATCAAATTTTTAAAATCATATAAATTTAAAGACAAAAAAGCAGAGCTTTATTACAAAGATTCATTAAATAAATTGTCAATAATTTTTGATCAAGATTTCAATAATAATGGCATTTTTTCAAATCTTTATAATGGGCATACAAATATTAATTTTAATAAAACTTTAACAGTAATAGATACTTATAACTTAATTAATAACATTGCTTCAAATGAGGGACAAGCATCATTATATTTGATACTCTCATATGTTAGAAACAGAATATCAAATAACTTTTTTGAAAACAAAACTAGAAAAATTTGTCTTATTGTAGATGAAGCTCATAGATTTATTAATGCTAATAATATGTCATCATTAGAATTTTTATTTGACACAGTCAAAACAATAAGAAAATATAATGGTTCTGTCATTCTAACTACACAAAATCCAAGCGATTTTGAATTAAGTGATGAAGTTATGAACTTAACTAAAGGGATGGTTGAAAACATACAATACACATTAATTTTTAGACAAAATCAAAATGGTGTCAATGCTATTAGTCAAATGTACAAATCAAAAAATGAATTAACAAATTGA", "aa": "MGKGLETKINPLQINYEIEKDNIKVSDELLISNHTKMVKEFFSLLYPDLDARDLRVANFLLNEFYLSNKIKNIQKLKNTDFPTMKDYIKFLKSYKFKDKKAELYYKDSLNKLSIIFDQDFNNNGIFSNLYNGHTNINFNKTLTVIDTYNLINNIASNEGQASLYLILSYVRNRISNNFFENKTRKICLIVDEAHRFINANNMSSLEFLFDTVKTIRKYNGSVILTTQNPSDFELSDEVMNLTKGMVENIQYTLIFRQNQNGVNAISQMYKSKNELTN", "aa_hexdigest": "280b7bca572fc66e13b2387afc433124", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05707.18", "name": "Zot", "length": 49, "aa_cov": 0.16967509025270758, "hmm_cov": 1.0, "evalue": 3.5195921609405798e-06, "score": 22.68251609802246, "start": 185, "stop": 231 } ], "seq_stats": { "molecular_weight": 32361.543500000033, "isoelectric_point": 8.832313728332519 }, "id": "OAIKOHHJFB_578", "locus": "GCA000209735_00552" }, { "type": "cds", "sequence": "contig_1", "start": 297749, "stop": 297850, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTCAATAGATAATAAAACAAGATTTCAAATTAGTATTTATTACAATCAATTCGAAAAAGATATGATTTACAAATCTGGTGATTTAAGTAACATTTAA", "aa": "MFSIDNKTRFQISIYYNQFEKDMIYKSGDLSNI", "aa_hexdigest": "035e85ec4bfd28e346bfa357462f8ecf", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4010.5033000000003, "isoelectric_point": 5.9190080642700185 }, "id": "OAIKOHHJFB_579", "locus": "GCA000209735_00553" }, { "type": "cds", "sequence": "contig_1", "start": 299557, "stop": 299673, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAATATCTAATTTAGCTCCAGGAAATGAAATCAAATTAACAAAAAATGGTTTTTATTTAATATATGACATATCACTAGATAAAACAAATATTAATAAATTTAAAATTGTTTGA", "aa": "MEISNLAPGNEIKLTKNGFYLIYDISLDKTNINKFKIV", "aa_hexdigest": "46b9446de2d42f5c527e5fc58df131d3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4359.047700000001, "isoelectric_point": 8.06391506195068 }, "id": "OAIKOHHJFB_580", "locus": "GCA000209735_00554" }, { "type": "cds", "sequence": "contig_1", "start": 299709, "stop": 299945, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATTTCTGGTTTGATTTCAAAATTTTCTTCAAAAGATTTTTCAATTAAAAAATTATTATACTCATTAAAAATATCATCATATTTTAATTTGTCAAGATCTTTTTTTGTTGCGTTTAAATCAATAATTTTTTTCTTGGCTAAGTATTTTTCAAAATCATTTGAAACTTTAAGATTTTTATTTCTTCAAAATTTTTTGATAAATGATGGAGAATTTGCTTCTTCTGTTAAATAA", "aa": "MLISGLISKFSSKDFSIKKLLYSLKISSYFNLSRSFFVAFKSIIFFLAKYFSKSFETLRFLFLQNFLINDGEFASSVK", "aa_hexdigest": "ef675762ed1fa72f7ae50d5f3eee181e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9093.62449999999, "isoelectric_point": 9.997321891784669 }, "id": "OAIKOHHJFB_581", "locus": "GCA000209735_00555" }, { "type": "cds", "sequence": "contig_1", "start": 300187, "stop": 300693, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAACCTATTTTTATTAAATTTAATAATATTAATATTGAAGAACATATACCATTCAATGTATTTGTTAATGAGTTAATTAATAATTTTAATAATTTGATTGAATCTGCTAAAGATATAAATAATGAAGACATTGACTTGAATATTATCGAATTAAAAATTTTGAAAGAAAGTGATGAAAATCAAATAATAGTTAAAGCTATATCAAAAGATGCAAATTATTATATAGCTAATAACGATACATATACATACAATGTAAATTTACTTAAATCAGATTATAGCTATTTATTGAATTTTAATCCTGGAACTTTAAATGTTTTTGGTTTAAAAGATAAAGAAGAAATAAAAAAATACATTAAAGAACTATTAAAAAATTATTTAATCCAATACAATGAAAAAATTGATCAATCTCTTTACAAAAAAATTACAATAGATGATATTAATATATCGTTTAATGATGAAGAGTTAAACAAAATAATTAATCCAAAAGATGATGACATTTATTGA", "aa": "MKPIFIKFNNINIEEHIPFNVFVNELINNFNNLIESAKDINNEDIDLNIIELKILKESDENQIIVKAISKDANYYIANNDTYTYNVNLLKSDYSYLLNFNPGTLNVFGLKDKEEIKKYIKELLKNYLIQYNEKIDQSLYKKITIDDINISFNDEELNKIINPKDDDIY", "aa_hexdigest": "25aa009585af386a0ed42f8f656a45a5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19855.2482, "isoelectric_point": 4.60994930267334 }, "id": "OAIKOHHJFB_582", "locus": "GCA000209735_00556" }, { "type": "cds", "sequence": "contig_1", "start": 300844, "stop": 301281, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAACAAAAAAGACCCTGTTAAAATGGCTCAAATTAAAAATGAGACTATTAGACAAGTTAGAGAAAAAATAATAGAAGAATTTAAAAAGAGAGCTCCAAAACTTGAAATTGATAAGTTATATACTATTGATTTTACCGATTCATTTTTCAATAATTTAGATTTAGCTATCGGAACAACCTTAACTTTAAAAGGCAAGCATTTTATGTTGAAAAACCCTTATACAGTTAATGTTATAAATACTCATTCATCAGCAAACAAAAAATTAAATTTAAAAGACTTAAATTTTGAAGTTATTACTTTAAGTGAAAAGAATTTAAATGATTTCAAAAAAGCTTTAAATGAAAAAATTATTGATATTCTCAAAAAAGAAAATTATCCTGCTAAAGAAATAATCAAATTAATTGAATTTAAATTATTAGAAAACCAAAAGTGA", "aa": "MENKKDPVKMAQIKNETIRQVREKIIEEFKKRAPKLEIDKLYTIDFTDSFFNNLDLAIGTTLTLKGKHFMLKNPYTVNVINTHSSANKKLNLKDLNFEVITLSEKNLNDFKKALNEKIIDILKKENYPAKEIIKLIEFKLLENQK", "aa_hexdigest": "8d500fe1206dd8cb856ae29ceacad26a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17024.737599999993, "isoelectric_point": 9.435415458679199 }, "id": "OAIKOHHJFB_583", "locus": "GCA000209735_00557" }, { "type": "cds", "sequence": "contig_1", "start": 301321, "stop": 301458, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGATTCAATTAAAAGAAGAATCAAATTTAATAAGTGGAGATAAAGAATTTAAATTAATTAATATTTTTACAGAAGAAATGTTAAAAGAAGCTTTAGAAATTAAGTCTAAAAACAAGAAAAAATTACTTTCATGA", "aa": "MMIQLKEESNLISGDKEFKLINIFTEEMLKEALEIKSKNKKKLLS", "aa_hexdigest": "4b6526b274932fbecbd98c07284bc658", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5284.2575, "isoelectric_point": 8.009826087951662 }, "id": "OAIKOHHJFB_584", "locus": "GCA000209735_00558" }, { "type": "cds", "sequence": "contig_1", "start": 301621, "stop": 301974, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_015510827.1", "SO:0001217", "UniParc:UPI0001A8C161", "UniRef:UniRef100_C4XEH7", "UniRef:UniRef50_C4XEH7", "UniRef:UniRef90_C4XEH7" ], "nt": "ATGAATTTACAAGATAAGCAAAATTATTATGAAAGTAATTTGAATTTTTCATTAAGTTCATTAATTATGAATGATGAAAATTCATTAAATAAATTCATTGCTATTAACTTAATCAAAAATATGATTAACCAATTAATTAATTATAAATATAGTGATGAAAGAAAAAGTAAATATAAAGCTCATGATGTGATTATAAGAATTGTGAGATTTGAAAATTTTACATTTAAATTGAAAGTAGAAACATATAAAGATAAAACAACAAATAAGTTGCATACATTTTATCCTGATTATTTAAATTTTGGATCATCTATATGTGAAGATGAAAAAGAAAGAATTCAAAATTATGCTGGTTAA", "aa": "MNLQDKQNYYESNLNFSLSSLIMNDENSLNKFIAINLIKNMINQLINYKYSDERKSKYKAHDVIIRIVRFENFTFKLKVETYKDKTTNKLHTFYPDYLNFGSSICEDEKERIQNYAG", "aa_hexdigest": "a3620e1f0bb895d371518d9065fe4c6b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C161", "ncbi_nrp_id": "WP_015510827.1", "uniref100_id": "UniRef100_C4XEH7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C161", "RefSeq:WP_015510827.1", "UniRef:UniRef100_C4XEH7" ] }, "ips": { "uniref100_id": "UniRef100_C4XEH7", "uniref90_id": "UniRef90_C4XEH7" }, "psc": { "uniref90_id": "UniRef90_C4XEH7", "uniref50_id": "UniRef50_C4XEH7" }, "pscc": { "uniref50_id": "UniRef50_C4XEH7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14028.73179999998, "isoelectric_point": 8.557420539855961 }, "id": "OAIKOHHJFB_585", "locus": "GCA000209735_00559" }, { "type": "gap", "sequence": "contig_1", "start": 302030, "stop": 302129, "strand": ".", "length": 100, "id": "OAIKOHHJFB_1801" }, { "type": "cds", "sequence": "contig_1", "start": 302454, "stop": 302732, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH8", "UniRef:UniRef90_C4XEH8" ], "nt": "TTGTTAGATATATTAAATACAATTTATCTCTATTTTAGAGACTCTTTTGTTTTGAGCGATATTGATAGATACAATTTAAATAGTTTGATAAGATACACATCTAAACTTGAAAGTGGAATCATTAATAATGATTATAGTTCACTAATAGAAGGACACATATCTGTTTATATTAAAAGAAAACTAAACAAAAGCTTTGCATTATATTCTCAAAAAACAATAGAAGCTTTATTGTTGAATGATAATAAAATTCAAAATACTCTTATTCAGACATATCATTAG", "aa": "MLDILNTIYLYFRDSFVLSDIDRYNLNSLIRYTSKLESGIINNDYSSLIEGHISVYIKRKLNKSFALYSQKTIEALLLNDNKIQNTLIQTYH", "aa_hexdigest": "5dd97fea0cd43f7cf7d9092425b8453f", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_C4XEH8", "query_cov": 1.0, "subject_cov": 0.8141592920353983, "identity": 0.9890000000000001, "score": 177.0, "evalue": 2.98e-56, "valid": true, "uniref50_id": "UniRef50_C4XEH8" }, "pscc": { "uniref50_id": "UniRef50_C4XEH8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH8" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10826.261399999988, "isoelectric_point": 7.949612617492677 }, "id": "OAIKOHHJFB_586", "locus": "GCA000209735_00560" }, { "type": "cds", "sequence": "contig_1", "start": 302985, "stop": 303116, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATATTATTAAAAGTGCTGGAAAAACTAGATCTGGAAATGTTGGGGTTTATGAAAATGGAACATTGATTCATATGGCTCCACCAGCTAATTTTGTAAACAATCTTTTAATAGATTTATTTGAATGA", "aa": "MNNIIKSAGKTRSGNVGVYENGTLIHMAPPANFVNNLLIDLFE", "aa_hexdigest": "29bc878b2b3c9cdc4c11e320477ea167", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4674.316799999999, "isoelectric_point": 6.532073402404785 }, "id": "OAIKOHHJFB_587", "locus": "GCA000209735_00561" }, { "type": "cds", "sequence": "contig_1", "start": 303219, "stop": 303629, "strand": "+", "frame": 3, "gene": null, "product": "Fido domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH9", "UniRef:UniRef90_C4XEH9" ], "nt": "TTGTGGCACAGATTTTTATTAAATAAAGAAAATAAATTATATGAAAATTTACAACTTGAAAATATAGTTTATGAAAATGTCAATAACTATTATAAAGCTTTACATAACTCAAACCAAAAAGGAGATTGCAGTGAATTTATTGAATGCATGTTAACTTTTATTTTAAAAGCTATTGAAAGAAATGAAATCTATGATTTTGATTCATTAAATGAAGTAGATAAATTGACTTCAATATTATCTTCAGGCCCTAAAAGTAGACAAGAAATTATGGATTTATTAAATATAAAATCAAGAATCACATTACAAAAAAGTTATATAAAACCGGCTTTAGAACAACAAAAAGTAATAATTATTGGTAAATTAAACTCACCTAAAGTAAAATATAAATTAGTGGAAAATAATAAGGAGTAA", "aa": "MWHRFLLNKENKLYENLQLENIVYENVNNYYKALHNSNQKGDCSEFIECMLTFILKAIERNEIYDFDSLNEVDKLTSILSSGPKSRQEIMDLLNIKSRITLQKSYIKPALEQQKVIIIGKLNSPKVKYKLVENNKE", "aa_hexdigest": "108e7a4df35dd109f76d4b3a54969ad4", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 302964, "stop": 303629, "observations": { "mutation": [ 303114 ], "stop": [ 303114 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEH9", "query_cov": 1.0, "subject_cov": 0.6153846153846154, "identity": 0.993, "score": 267.0, "evalue": 2.02e-89, "gene_start": 1, "gene_end": 136, "reference_start": 86, "reference_end": 221, "reference_sequence": "MKIHKILMNNIIKSAGKTRSGNVGVYENGTLIHMAPPANFVNNLLIDLFEWYNNDISDVLIKSCAFHYELEFIHPFEDGNGRIGRLWHRFLLNKENKLYENLQLENIVYENVNNYYKALHNSNQKGDCSEFIECMLTFILKAIERNEIYDFDSLNEVDKLTSILSSGPKSRQEIMDLLNIKSRITLQKSYIKPALEQQKVIIIGKLNSPKVKYKLVENNKE" }, "paralog": false, "identity": 0.9909502262443439, "score": 441.0, "evalue": 1.77e-160, "description": "Internal stop codon at 303114. Nonsense mutation around 303114.", "psc": { "uniref90_id": "UniRef90_C4XEH9", "product": "Fido domain-containing protein", "uniref50_id": "UniRef50_C4XEH9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEH9", "UniRef:UniRef50_C4XEH9" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEH9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH9" ], "product": "Fido domain-containing protein" } }, "id": "OAIKOHHJFB_588", "locus": "GCA000209735_00562" }, { "type": "cds", "sequence": "contig_1", "start": 303633, "stop": 303770, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCAAATTTATAATTGATAAAAGTTTTTTTGAACTTTTTCCTCAAGCTAAACTTGGAGTAGTTTTAATTAATGATATTAAAAGTAATTCAAACAGTCCAAAAGAGCTGCAAGACTATTTAAGAAAAAGTACTTGA", "aa": "MSKFIIDKSFFELFPQAKLGVVLINDIKSNSNSPKELQDYLRKST", "aa_hexdigest": "b1c15633e11f4ecc7a0bc847eb4c2168", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5190.9633, "isoelectric_point": 9.22402362823486 }, "id": "OAIKOHHJFB_589", "locus": "GCA000209735_00563" }, { "type": "cds", "sequence": "contig_1", "start": 303858, "stop": 304145, "strand": "+", "frame": 3, "gene": null, "product": "B3/4 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000C0199FF" ], "nt": "ATGAAAAAAGCAAGATCAAGTGTTGAAATGATGCTTAAAAGAGCAAGTAAAGACGCACAATTTTCTTCAATTAACCCTTTAGTTGATATATACAATAGTGTTGGCTTACGTTTTGCATTTCCTTGTGGCGCAGAGGATATTAATAAATTTAGTGGCAATTTAATTTTAGGCATTACCAAAGGTGGAAATGAATTTTATAGAATTAATAGCACTGAAAATGAACCTGCTTTAGCAGGAGAGTTATGTTATTATGATGATAAAGGTGCTGTTTGTCGTAATTTTAATTGA", "aa": "MKKARSSVEMMLKRASKDAQFSSINPLVDIYNSVGLRFAFPCGAEDINKFSGNLILGITKGGNEFYRINSTENEPALAGELCYYDDKGAVCRNFN", "aa_hexdigest": "75375675ebb57b0e571a599c28ce7cbf", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000C0199FF", "query_cov": 0.968421052631579, "subject_cov": 0.8679245283018868, "identity": 0.5329999999999999, "score": 102.0, "evalue": 1.97e-26, "valid": false, "product": "B3/4 domain-containing protein", "uniref50_id": "UniRef50_UPI000C0199FF" }, "pscc": { "uniref50_id": "UniRef50_UPI000C0199FF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000C0199FF" ], "product": "B3/4 domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_590", "locus": "GCA000209735_00564" }, { "type": "cds", "sequence": "contig_1", "start": 304468, "stop": 305055, "strand": "+", "frame": 1, "gene": null, "product": "Abortive phage infection protein", "db_xrefs": [ "RefSeq:WP_013526752.1", "SO:0001217", "UniParc:UPI0001F5A11B", "UniRef:UniRef100_C4XEI1", "UniRef:UniRef50_C4XEI1", "UniRef:UniRef90_C4XEI1" ], "nt": "ATGAACTACAATGAAAAACTTGAAAAAGTTATTGAAGAATGTAATGGAGTTGTTACTACTAAAGAATGTAATAAAAGAAATATTCCTAATTCAATAATTTCAAGAGCTGCCAAAAAAGGCAGAATTGAAAAATATTCTGAAGGAATTTATGTTGCTGAAGAAATACCAATGGATCCACACTTTCTTTTTCAACTTCGATTTCCAAGAAGTATTTTTTCTTATGAAAGTGCATTGCAACTTCATGGAGTGAGTGAAAGATTTGAAAAAGCGCTAGAAGTCACAGTAAATACAGGATATAAATTTAATGATACTAAGAAAATCTTTCCGCACTATGTAAAAGATGAGTATCTTGAATTGGGAGTAGAAGAAATAAAAACAAGTCAAGGCAATAAGGTTAGAGTTTATTCTTTGGAAAGAACTTTTTGCGATATGGTAGCTAATAGAAATAAAGTAGATGCTGAAACTTTTGTAAAATTTATGATTACATTCCAAGGAATAAAAATAAATTATTCTCAACTTAATAGTATTGCTAAAAAAATGGGAGTCATTAACAAAGTTGTTGATGTAATGGGAATAATTCGTGAATAA", "aa": "MNYNEKLEKVIEECNGVVTTKECNKRNIPNSIISRAAKKGRIEKYSEGIYVAEEIPMDPHFLFQLRFPRSIFSYESALQLHGVSERFEKALEVTVNTGYKFNDTKKIFPHYVKDEYLELGVEEIKTSQGNKVRVYSLERTFCDMVANRNKVDAETFVKFMITFQGIKINYSQLNSIAKKMGVINKVVDVMGIIRE", "aa_hexdigest": "939390d8773cf80f17abb4e440145976", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001F5A11B", "ncbi_nrp_id": "WP_013526752.1", "uniref100_id": "UniRef100_C4XEI1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5A11B", "RefSeq:WP_013526752.1", "UniRef:UniRef100_C4XEI1" ] }, "ips": { "uniref100_id": "UniRef100_C4XEI1", "uniref90_id": "UniRef90_C4XEI1" }, "psc": { "uniref90_id": "UniRef90_C4XEI1", "product": "Abortive phage infection protein", "uniref50_id": "UniRef50_C4XEI1" }, "pscc": { "uniref50_id": "UniRef50_C4XEI1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEI1" ], "product": "Abortive phage infection protein" }, "genes": [], "id": "OAIKOHHJFB_591", "locus": "GCA000209735_00565" }, { "type": "cds", "sequence": "contig_1", "start": 305081, "stop": 305785, "strand": "+", "frame": 2, "gene": null, "product": "Abortive infection protein AbiGII", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEI2", "UniRef:UniRef90_C4XEI2" ], "nt": "TTGGTTTCAAAGAAATTTGATATTGATTACAATTCCATAAGAAAATTATTTTATCTTGAAGAAATACTAAAAAGAATTGCAAAAAGTAAATATAGAAATAATTTTATTTTTAAAGGCGGGATGTTGTTTTCAAACATAATAGGTATTGAAAATAGAAGAACTGTTGATATTGATTTTAGTTTGAAAGGTATAGAGTCTAATGAAAAAAATTTAAAAATTGTTTTTAATGAAATATTGTTAATAAAAAATGATGAAATAAATTTTATTTTTAATGATATACAACCTATAAAAACTGATTTAAATTATCCTTGTTACAGATTTAAATTTAGAGCTTCCTTAGAAAACGAGAAAGAAGATATTGTTCTTGATATTGGTGTTGGAGATAAAATATTTCCAAAACCAATTGAATACTCTTATAAAAGTTTTTTTAAAGATGAAAGTTTTCAAGTTTTGTCATACACTCTTGAAACATATTTTTCAGAAAAACTTTTTGCAATAATTTCTAAAGGAGAAGCTAATACAAGACTAAAAGATTATTATGATTTACATTTTTTAATATTAAATTGTAAAAAAATGATTGATAAATCAAATTTAGGCAAAGCAATTTCGATAACATTTGATAATAGAAATGAAAAATTTGACATTAAAACTGCAAAATTAAAACTTTTAGAGTTATCTAATTTTGAGTTATTGGATAATAAATGA", "aa": "MVSKKFDIDYNSIRKLFYLEEILKRIAKSKYRNNFIFKGGMLFSNIIGIENRRTVDIDFSLKGIESNEKNLKIVFNEILLIKNDEINFIFNDIQPIKTDLNYPCYRFKFRASLENEKEDIVLDIGVGDKIFPKPIEYSYKSFFKDESFQVLSYTLETYFSEKLFAIISKGEANTRLKDYYDLHFLILNCKKMIDKSNLGKAISITFDNRNEKFDIKTAKLKLLELSNFELLDNK", "aa_hexdigest": "9028a0862dc82d4cacd999c7fbaffc0f", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XEI2", "query_cov": 1.0, "subject_cov": 0.8447653429602888, "identity": 0.996, "score": 459.0, "evalue": 1.52e-162, "valid": true, "product": "Abortive infection protein AbiGII", "uniref50_id": "UniRef50_C4XEI2" }, "pscc": { "uniref50_id": "UniRef50_C4XEI2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEI2" ], "product": "Abortive infection protein AbiGII" }, "genes": [], "id": "OAIKOHHJFB_592", "locus": "GCA000209735_00566" }, { "type": "cds", "sequence": "contig_1", "start": 305930, "stop": 306319, "strand": "-", "frame": 1, "gene": null, "product": "Enolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A139PDG5" ], "nt": "ATGACAACTAAATACGGGCACCAAATTCAAATCGTTGGTGATGACTTAACAGTTACAAACCCTAAATTATTAGCGCGTGCTATTAAAGAAAAAGCAATGAATGCAATTTTAATTAAATTAAACCAAATTGGTTCATTAACAGAAACAATTGATGCTATTAACATGGCTCAAAAAGCTGGAATGGCTTGTGTTGTTTCACACCGTTCAGGTGAAACAGAAGACACAACATTAGCTGATGTTGCTGTTGCATTAAACACAGGACAAATCAAAACTGGATCAATGTCACGTACAGATAGAATTGCAAAATACAATAGATTATTAGCTATTGAAGAAGAATTAGATGGTGCTGATGAATTTGAAGGTCGTGAAGCATTCTACAACATTAAATAA", "aa": "MTTKYGHQIQIVGDDLTVTNPKLLARAIKEKAMNAILIKLNQIGSLTETIDAINMAQKAGMACVVSHRSGETEDTTLADVAVALNTGQIKTGSMSRTDRIAKYNRLLAIEEELDGADEFEGREAFYNIK", "aa_hexdigest": "5b4f7b6538a069aadc8a2cdbe95c8244", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A139MRN9", "query_cov": 1.0, "subject_cov": 0.8376623376623377, "identity": 0.628, "score": 164.0, "evalue": 1.48e-49, "valid": false, "product": "Enolase", "uniref50_id": "UniRef50_A0A139PDG5" }, "pscc": { "uniref50_id": "UniRef50_A0A139PDG5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A139PDG5" ], "product": "Enolase" }, "genes": [], "id": "OAIKOHHJFB_593", "locus": "GCA000209735_00567" }, { "type": "cds", "sequence": "contig_1", "start": 306335, "stop": 307090, "strand": "-", "frame": 1, "gene": "eno", "product": "phosphopyruvate hydratase", "db_xrefs": [ "EC:4.2.1.11", "GO:0000015", "GO:0000287", "GO:0004634", "GO:0006096", "KEGG:K01689", "SO:0001217", "UniRef:UniRef50_P75189", "UniRef:UniRef90_C4XEI3" ], "nt": "ATGACCGCAGTTGATCATGTTAACAAAGATATTGCACCTAAATTATTAGGAATGGAAGTTACAGATCAACGTGCAATCGACATGAAAATGATTAAATTAGATGGCACAGCAACAAAATCAAAATTTGGTGCTAACGCTATCTTAGGAGTTTCATTAGCAGTAGCTAGAGCAGCCGCAGATGAATTACAACTTCCTTTATATAGATACATTGGTGGTACAAATGCCCACGTATTACCATTACCTATGTTAAATGTTATGAATGGTGGAGCTCACGCATCAAACACAGTTGACTTCCAAGAATTCATGATTATGCCAGTTGGTGCTAAAAGCATTCGTGAAGCATTACAAATGGCAAACAAAGTATTCCATAACTTAGCAAAATTATTAAAAGCTGCAGGACACGGAACACAAGTTGGTGATGAAGGTGGATTTGCACCTGACTGCAAAAGTCATGAAGAAGTTCTTGACTACTTAGTTCAAGCTATTAAGGCTGCAGGTTACAAACCAGCTACAAGCGGTAAAGATGCTGTTGCTATTGCAATGGATGCTGCTTCAAGTGAAATTTACGACGAAAAAAGCAAAAAATATGTATTCAAAAAACTTAAAAAAGCTATTGAAGCTAAACACCCAGGTTTTGAAAAACTTACAAATGTTAAACTTGAATACACAACAGATGAAATGATTGAATACTATGGTACTTTAATTGCTAAATACCCAATTATCTCAATCGAAGATGGTTTAGCAGAAGATGACTGA", "aa": "MTAVDHVNKDIAPKLLGMEVTDQRAIDMKMIKLDGTATKSKFGANAILGVSLAVARAAADELQLPLYRYIGGTNAHVLPLPMLNVMNGGAHASNTVDFQEFMIMPVGAKSIREALQMANKVFHNLAKLLKAAGHGTQVGDEGGFAPDCKSHEEVLDYLVQAIKAAGYKPATSGKDAVAIAMDAASSEIYDEKSKKYVFKKLKKAIEAKHPGFEKLTNVKLEYTTDEMIEYYGTLIAKYPIISIEDGLAEDD", "aa_hexdigest": "7db3fce6604d83ecc25876a608756bf6", "start_type": "ATG", "rbs_motif": "GGxGG", "genes": [ "eno" ], "truncated": "both", "pseudogene": { "start": 306032, "stop": 307312, "observations": { "mutation": [ 306337, 307111, 307219 ], "stop": [ 306337, 307111, 307219 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEI3", "query_cov": 1.0, "subject_cov": 0.5456521739130434, "identity": 1.0, "score": 487.0, "evalue": 1.52e-170, "gene_start": 1, "gene_end": 251, "reference_start": 75, "reference_end": 325, "reference_sequence": "MKLRRKMSAIYKIHSREILDSRGNPTVQVEVWTEAGGYGLANVPSGASTGSKEALELRDQGTKYEKNWFGGKGVMTAVDHVNKDIAPKLLGMEVTDQRAIDMKMIKLDGTATKSKFGANAILGVSLAVARAAADELQLPLYRYIGGTNAHVLPLPMLNVMNGGAHASNTVDFQEFMIMPVGAKSIREALQMANKVFHNLAKLLKAAGHGTQVGDEGGFAPDCKSHEEVLDYLVQAIKAAGYKPATSGKDAVAIAMDAASSEIYDEKSKKYVFKKLKKAIEAKHPGFEKLTNVKLEYTTDEMIEYYGTLIAKYPIISIEDGLAEDDWAGFTKMTTKYGHQIQIVGDDLTVTNPKLLARAIKEKAMNAILIKLNQIGSLTETIDAINMAQKAGMACVVSHRSGETEDTTLADVAVALNTGQIKTGSMSRTDRIAKYNRLLAIEEELDGADEFEGREAFYNIK" }, "paralog": false, "identity": 0.9906103286384976, "score": 819.0, "evalue": 6.25e-304, "description": "Internal stop codon at 306337, 307111, 307219. Nonsense mutation around 306337, 307111, 307219.", "psc": { "uniref90_id": "UniRef90_C4XEI3", "gene": "eno", "product": "phosphopyruvate hydratase", "ec_ids": [ "4.2.1.11" ], "uniref50_id": "UniRef50_P75189", "kegg_orthology_id": "K01689", "go_ids": [ "GO:0000015", "GO:0000287", "GO:0004634", "GO:0006096" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEI3", "EC:4.2.1.11", "UniRef:UniRef50_P75189", "KEGG:K01689", "GO:0000015", "GO:0000287", "GO:0004634", "GO:0006096" ] }, "pscc": { "uniref50_id": "UniRef50_P75189", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75189" ], "product": "Enolase" } }, "id": "OAIKOHHJFB_594", "locus": "GCA000209735_00568" }, { "type": "cds", "sequence": "contig_1", "start": 307589, "stop": 308017, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATTGCTTACTTTTGGTATGAAAAAGGAAAATCAAGACATTGATTCTGCTTTTTCTAGACCATTGCAATTTATTTCTAATAAAGTCATTGCAAAACTTGGAGTAGGAAGCCATTTAATCAATGATTATACTGTTACAGCTTTAGGACATGCCTTCTGTGACTCAATTGTAGAATCTGAAAAAAATAATAAAGAATTCAAAATATTTTTATCAAATGATAACACATTTCATGCTTTACTTTATACAAATATTTTGGCTCGTATTTTTGACGAAAAAGGTTTTAAATCATACATATTTGAAAAAAATAGTTCATATCCAAGCGTACTAAAAAACTTGGCTGCAAAAGACGATGATTGTGATGTAATTATTAGTGTTGAAAGCTTTAAAGGTCTAAAAAATATTATGCAAATTAGTTTCAATTGA", "aa": "MKKLLTFGMKKENQDIDSAFSRPLQFISNKVIAKLGVGSHLINDYTVTALGHAFCDSIVESEKNNKEFKIFLSNDNTFHALLYTNILARIFDEKGFKSYIFEKNSSYPSVLKNLAAKDDDCDVIISVESFKGLKNIMQISFN", "aa_hexdigest": "2b03d5f235cc455860a869c3ad8112e1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16085.282099999982, "isoelectric_point": 7.737003135681152 }, "id": "OAIKOHHJFB_595", "locus": "GCA000209735_00569" }, { "type": "cds", "sequence": "contig_1", "start": 308069, "stop": 308362, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTCAGTAAATTATTTAAGTATAGACATTCCAAATCAAGGAATTAGTTTCAAATATAATCACTCTCCTTTAAATTATAATCGCGAAATCTTAAAACGTTATTCACCTTTAATTAGTACATTTAAAAAGCAAAATTTAAAATTCGGTGTTGATATTTCACAACTATCAACAGCTAATTTTTACTATGATACTTTAAATAGATTACAAATTGACTACTTTACTCATAATCGTAAAAAAGATGCTTACATTATTAATGCAAATAATCCAGATTGTTTAAAAAAAATATTCTGA", "aa": "MNSVNYLSIDIPNQGISFKYNHSPLNYNREILKRYSPLISTFKKQNLKFGVDISQLSTANFYYDTLNRLQIDYFTHNRKKDAYIINANNPDCLKKIF", "aa_hexdigest": "c189ee9498c31e6d549ab6f99fd898df", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11473.931199999983, "isoelectric_point": 9.519159889221193 }, "id": "OAIKOHHJFB_596", "locus": "GCA000209735_00570" }, { "type": "cds", "sequence": "contig_1", "start": 308759, "stop": 309202, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGAGTTAATGAGAATGATTGCTTTCTATAAAGAAAAATCATTAACTCTTTATGAAGTTTTACAACAAATTTATGATAAATATGGATTGCATCATGTTATCACTAAAACATTTAATTTAGATGATGAAATGGCTTCGAGATTTATTAAAAGAATACTTAAATCTGAAACAATTGGTAACCATAAAATAGTTAACTTTAAAGAATACAAAAATGCCACAATAGTCAATAATAATATCCATTTAAAGCTTTCTTTTGAAGGTGGCAATAGTATTACTGTTAAGTATTCTATAATTGAGAAAGAAATAACTATAGACTGTGAAGCAATAGCTAAAACTAATAGTCAAGAAGATCGGACCAATGTTGTAATTTTAGAAAGAGAACTTATGGATGGAATTTTAGAATTAAAAGAGGACTTTAAAATTCGCAAAATTACACCATGA", "aa": "MLELMRMIAFYKEKSLTLYEVLQQIYDKYGLHHVITKTFNLDDEMASRFIKRILKSETIGNHKIVNFKEYKNATIVNNNIHLKLSFEGGNSITVKYSIIEKEITIDCEAIAKTNSQEDRTNVVILERELMDGILELKEDFKIRKITP", "aa_hexdigest": "1f67790d191061df217ae854bdf05d66", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17229.84289999999, "isoelectric_point": 6.914940834045409 }, "id": "OAIKOHHJFB_597", "locus": "GCA000209735_00571" }, { "type": "cds", "sequence": "contig_1", "start": 309230, "stop": 309472, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTTTTATTGGAGTCATTATCTTCCTTTACTTTAGTATTTATAACTTCAAAGATGATAGTTTCTTTGGGGCTAATGGTGATATGGGTGAAATGTTCAAGCGGATGGGAATAGTAATTTTTAAAGATCACAAAACGCAGTTTGCATTCTTATCAATTGTTCTTTCGTTTTTTATTTGGACCTTATTTAATGCTATTATCTTCAAACGGCTTCTCGATTTCCAAGGTCAAAAAGTCAAATGA", "aa": "MTFIGVIIFLYFSIYNFKDDSFFGANGDMGEMFKRMGIVIFKDHKTQFAFLSIVLSFFIWTLFNAIIFKRLLDFQGQKVK", "aa_hexdigest": "be23b2457f79a1601612a08498fcf622", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9455.13379999999, "isoelectric_point": 9.455400657653808 }, "id": "OAIKOHHJFB_598", "locus": "GCA000209735_00572" }, { "type": "cds", "sequence": "contig_1", "start": 310325, "stop": 310525, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGGTCTTCATTGGTGAAAAGAGAGTTGACTTATATCAAATTAAAGAAAAGAATTTAAGACTTATTCAAAATGACAATATGTCAGCAACCACTAGAACAAAAACTAGATTTTACAAAATTAGCTTTACAATTTATACAATAATTATTATTGCTTTAGCAATTACAATATTATGCCCTCTAATGGGATCATATAAGTAG", "aa": "MLVFIGEKRVDLYQIKEKNLRLIQNDNMSATTRTKTRFYKISFTIYTIIIIALAITILCPLMGSYK", "aa_hexdigest": "a44c1c53148b18455fec6649fea04e5f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7718.2154999999975, "isoelectric_point": 9.841888618469238 }, "id": "OAIKOHHJFB_599", "locus": "GCA000209735_00573" }, { "type": "cds", "sequence": "contig_1", "start": 310762, "stop": 311433, "strand": "+", "frame": 1, "gene": null, "product": "GTP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF59E5" ], "nt": "ATGAAAGACTGCACATCGGTTGGTTTTAGCGCTAAAATACAATTTATTAATAAAATGAAAGGAAAATATTACATGGCAAAACAAGATTTTAATCGTAATAAAGACCACGTTAATATTGGTACCATTGGTCACGTTGACCACGGTAAAACTACTTTAACCGCTGCTATTGCTACAGTATTATCAAAGAAGGGTCTTGCAGAAGCTAAAGACTATGCTGCTATTGATAATGCACCAGAAGAAAAAGCTCGTGGTATAACAATTAATACATCACACATCGAATACGAAACTGAAAAACGTCACTATGCACACGTTGACTGTCCAGGTCACGCTGACTACATCAAAAACATGATTACAGGTGCTGCACAAATGGATGGTGCTATCTTAGTTGTTGCTGCAACAGATGGTGCTATGCCTCAAACACGTGAACACATTCTTCTTTCAAAACAAGTTGGTGTTCCTCGTATGGTTGTTTTCTTAAACAAATGCGACATGCTTAAAGGTGAAGAAGAAATGATCGAACTTGTTGAAATGGAAGTTCGTGAATTACTTTCAAAATATGGATTTGACGGAGACAACACTCCAGTTATCCGTGGTTCAGCTTTAGAAGCTCTTAAAGGTAACAAAGAATACGAAGACAAAATTATGGAATTAATGAATGCTGTTGACACATGA", "aa": "MKDCTSVGFSAKIQFINKMKGKYYMAKQDFNRNKDHVNIGTIGHVDHGKTTLTAAIATVLSKKGLAEAKDYAAIDNAPEEKARGITINTSHIEYETEKRHYAHVDCPGHADYIKNMITGAAQMDGAILVVAATDGAMPQTREHILLSKQVGVPRMVVFLNKCDMLKGEEEMIELVEMEVRELLSKYGFDGDNTPVIRGSALEALKGNKEYEDKIMELMNAVDT", "aa_hexdigest": "3e01d601cd1c824d862be759c3db4bc3", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI0021AF59E5", "query_cov": 0.874439461883408, "subject_cov": 0.8297872340425532, "identity": 0.836, "score": 335.0, "evalue": 1.73e-114, "valid": false, "product": "GTP-binding protein", "uniref50_id": "UniRef50_UPI0021AF59E5" }, "pscc": { "uniref50_id": "UniRef50_UPI0021AF59E5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF59E5" ], "product": "GTP-binding protein" }, "genes": [], "id": "OAIKOHHJFB_600", "locus": "GCA000209735_00574" }, { "type": "cds", "sequence": "contig_1", "start": 311473, "stop": 312021, "strand": "+", "frame": 1, "gene": "tuf", "product": "elongation factor Tu", "db_xrefs": [ "GO:0003746", "GO:0005525", "GO:0006414", "SO:0001217", "UniRef:UniRef50_O83217", "UniRef:UniRef90_C4XEI5" ], "nt": "ATGGCTGTTGAAGACGTTTTCACAATCACAGGTCGTGGTACAGTTGCTACAGGACGTGTTGAACGTGGAAGATTAAACCTTAACGAAGAAGTTGAAATTGTTGGTTTACACCCAACTAAGAAAACTGTTGTTACAGGTATGGAAATGTTCCGTAAAAACCTTAAAGAAGTTCAAGCTGGTGACAACGCTGGTTTATTACTTCGTGGTATTGAAAGAGCAGGAATCGAACGTGGACAAGTTCTTGCTAAACCAGGAACAATCATTCCTCACACAGAATTTACAGCAGCAATTTACGTATTAACCAAAGATGAAGGTGGACGTCACACACCATTCTTCAAAAACTATAAACCACAATTCTACTTCCGTACAACAGACGTTACAGGTGGTGTTGAATTTGAAAAAGGCCGTGAAATGGTTACACCAGGGGAAAATGTTAACTTAACAGTTAAACTTATCTCACCTATTGCCGTTGAAAACGGAACTAAATTCTCAATTCGTGAAGGTGGACGTACAGTTGGTTATGGTAATGTAACAAAAATTATTAAATAA", "aa": "MAVEDVFTITGRGTVATGRVERGRLNLNEEVEIVGLHPTKKTVVTGMEMFRKNLKEVQAGDNAGLLLRGIERAGIERGQVLAKPGTIIPHTEFTAAIYVLTKDEGGRHTPFFKNYKPQFYFRTTDVTGGVEFEKGREMVTPGENVNLTVKLISPIAVENGTKFSIREGGRTVGYGNVTKIIK", "aa_hexdigest": "ae0171f1ba10dc3f8e3ac9906b75c5a6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00211B1E30", "query_cov": 1.0, "subject_cov": 0.9479166666666666, "identity": 0.7090000000000001, "score": 270.0, "evalue": 2.93e-90, "valid": false, "product": "EF-Tu/IF-2/RF-3 family GTPase", "uniref50_id": "UniRef50_A0A0A8KZ15" }, "genes": [ "tuf" ], "truncated": "5-prime", "pseudogene": { "start": 311173, "stop": 312021, "observations": { "mutation": [ 311431 ], "stop": [ 311431 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEI5", "query_cov": 1.0, "subject_cov": 0.41935483870967744, "identity": 1.0, "score": 365.0, "evalue": 3.78e-124, "gene_start": 1, "gene_end": 182, "reference_start": 253, "reference_end": 434, "reference_sequence": "MFIKVILVNSMDRMQMKDCTSVGFSAKIQFINKMKGKYYMAKQDFNRNKDHVNIGTIGHVDHGKTTLTAAIATVLSKKGLAEAKDYAAIDNAPEEKARGITINTSHIEYETEKRHYAHVDCPGHADYIKNMITGAAQMDGAILVVAATDGAMPQTREHILLSKQVGVPRMVVFLNKCDMLKGEEEMIELVEMEVRELLSKYGFDGDNTPVIRGSALEALKGNKEYEDKIMELMNAVDTWIQTPVKEFDKPFLMAVEDVFTITGRGTVATGRVERGRLNLNEEVEIVGLHPTKKTVVTGMEMFRKNLKEVQAGDNAGLLLRGIERAGIERGQVLAKPGTIIPHTEFTAAIYVLTKDEGGRHTPFFKNYKPQFYFRTTDVTGGVEFEKGREMVTPGENVNLTVKLISPIAVENGTKFSIREGGRTVGYGNVTKIIK" }, "paralog": false, "identity": 0.9964539007092199, "score": 556.0, "evalue": 7.35e-202, "description": "Internal stop codon at 311431. Nonsense mutation around 311431.", "psc": { "uniref90_id": "UniRef90_C4XEI5", "gene": "tuf", "product": "elongation factor Tu", "uniref50_id": "UniRef50_O83217", "go_ids": [ "GO:0003746", "GO:0005525", "GO:0006414" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEI5", "UniRef:UniRef50_O83217", "GO:0003746", "GO:0005525", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_O83217", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O83217" ], "product": "Elongation factor Tu" } }, "id": "OAIKOHHJFB_601", "locus": "GCA000209735_00575" }, { "type": "cds", "sequence": "contig_1", "start": 312146, "stop": 312547, "strand": "+", "frame": 2, "gene": "tsaE", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE", "db_xrefs": [ "GO:0002949", "RefSeq:WP_013526755.1", "SO:0001217", "UniParc:UPI0001F5A25F", "UniRef:UniRef100_C4XEI6", "UniRef:UniRef50_A0A449B9H2", "UniRef:UniRef90_C4XEI6" ], "nt": "ATGAAAAATTTAAAAACTTTTATAACTAGAGATGTTAAAGTAGTTAAAAAAGTAGCTCAATATGTTTTAGATCATTTAACTAAAAGCAAATTAGTTTTGATGAATGGCGACTTAGGAGCTGGTAAAACAACTTTAACTAAAGAAATAGCTAAATTATTAAGCATTGATGAAGTTATTACTTCACCAACTTTTAACTATATGAAAGTGTATGATGGTCTTGTTCATATCGATGCTTATAATCTTAAAGGCGATATTAGTGAATTTGAAGATTACTTTGAAGATAATGTTGTTGTTATTGAATGGGCTAATAGAATCAAGCATTATTATAAAAACTATTTAGATATCAATATTACTTTAGATAAAGACAACAATCATGTATTTACTATTAAGGAGGCTAAATAA", "aa": "MKNLKTFITRDVKVVKKVAQYVLDHLTKSKLVLMNGDLGAGKTTLTKEIAKLLSIDEVITSPTFNYMKVYDGLVHIDAYNLKGDISEFEDYFEDNVVVIEWANRIKHYYKNYLDINITLDKDNNHVFTIKEAK", "aa_hexdigest": "e3374e7e7779f815b6d8b5972d02e756", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001F5A25F", "ncbi_nrp_id": "WP_013526755.1", "uniref100_id": "UniRef100_C4XEI6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5A25F", "RefSeq:WP_013526755.1", "UniRef:UniRef100_C4XEI6" ] }, "ips": { "uniref100_id": "UniRef100_C4XEI6", "uniref90_id": "UniRef90_C4XEI6" }, "psc": { "uniref90_id": "UniRef90_C4XEI6", "gene": "tsaE", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE", "uniref50_id": "UniRef50_A0A449B9H2", "go_ids": [ "GO:0002949" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449B9H2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B9H2" ], "product": "tRNA threonylcarbamoyladenosine biosynthesis protein TsaE" }, "genes": [ "tsaE" ], "id": "OAIKOHHJFB_602", "locus": "GCA000209735_00576" }, { "type": "cds", "sequence": "contig_1", "start": 312547, "stop": 313107, "strand": "+", "frame": 1, "gene": "tsaB", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB", "db_xrefs": [ "EC:2.3.1.234", "GO:0002949", "RefSeq:WP_013354458.1", "SO:0001217", "UniParc:UPI0001E33008", "UniRef:UniRef100_C4XEI7", "UniRef:UniRef50_N9VF08", "UniRef:UniRef90_C4XEI7" ], "nt": "ATGAAGTTATATTTAGATACAGCCAATGAAGACTTTGTTTTAGCTTTGCTTAATGATAAACATCAAGTAATAGCTCAAAAAGTTTTAGAATCATATCCTAAAAAAGTCGAATTGATACCCCAATTAACAGAAGAAATATTAAAAGAAAATAATTTTAAAATTCAAGATTTTGATACTTTTTATATTAATATAGGACCTGGTTTTTTTACAGGGGTTAGAATTGCTTTAGTTTATTTAAGAACTATAGTTTTAATTACAAAAGCTAATATTAAAACTATTAGTACTATGCAAATATTAGCTAAACAAAACAGAAGAAAAAGAAGTTTTAAAATTAATGCTCAAGGCAATAAATACTATTTATATGAAACTAATAAAAATAGTGCATTTAATTATAAAAATATTAAAGTTGAAACAGGTACTTTAAAAGCCTATGACAAAGTTAATTATTTTGACTTTTTAAGCTGTTTTATAGATTATTTGGACGAATTCAAAAGCTATAAAGATGTAATGGATATTGAACCTTATTACATTAAAATGCCACAGATAGGAGCTAAAAAATAA", "aa": "MKLYLDTANEDFVLALLNDKHQVIAQKVLESYPKKVELIPQLTEEILKENNFKIQDFDTFYINIGPGFFTGVRIALVYLRTIVLITKANIKTISTMQILAKQNRRKRSFKINAQGNKYYLYETNKNSAFNYKNIKVETGTLKAYDKVNYFDFLSCFIDYLDEFKSYKDVMDIEPYYIKMPQIGAKK", "aa_hexdigest": "7a8f44009b96a1347d13d10bc328d8dc", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001E33008", "ncbi_nrp_id": "WP_013354458.1", "uniref100_id": "UniRef100_C4XEI7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33008", "RefSeq:WP_013354458.1", "UniRef:UniRef100_C4XEI7" ] }, "ips": { "uniref100_id": "UniRef100_C4XEI7", "uniref90_id": "UniRef90_C4XEI7" }, "psc": { "uniref90_id": "UniRef90_C4XEI7", "gene": "tsaB", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_N9VF08", "go_ids": [ "GO:0002949" ] }, "pscc": { "uniref50_id": "UniRef50_N9VF08", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9VF08" ], "product": "Gcp-like domain-containing protein" }, "genes": [ "tsaB" ], "id": "OAIKOHHJFB_603", "locus": "GCA000209735_00577" }, { "type": "cds", "sequence": "contig_1", "start": 313107, "stop": 314033, "strand": "+", "frame": 3, "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "db_xrefs": [ "EC:2.3.1.234", "GO:0002949", "SO:0001217", "UniRef:UniRef50_Q4A734", "UniRef:UniRef90_C4XEI8" ], "nt": "ATGCGTATTTTAGGAATAGAAACAAGCCATGATGATACTAGTATAGCCCTCTTAGAAGATGGTAAAGTGTTAGCTTTAAAAACTATTAGTCAAATAGATATTTTCAAAGAATTTGGTGGCACAATTCCTGAAATATCTTCAAGAGAACATGTTAAAAATATTAACTTAATTGAAAAAATATTTTTACAAGAATTTGATCTTAATACTATTGACTACATTGCTTATACTCAAAAACCTGGATTAATCGGAACCTTGCAAATAGGTTTCTTATTTGCTAGTGCTTTAGCTCTTGCTTTAAATAAACCTCTTTTACCTATTAATCACCTTGAAGGTCACTTTTACTCAGCAGCTATTAAACAAGAAATAAAATATCCTGCATTAAGCTTATTAGTATCAGGTGGTCATACTCAATTGATGTTAGTTAAAAGTCCTTTTGACATTCAAATAATTGGACAAACACTAGATGATGCTGTTGGTGAAGCTTATGATAAAGTAGCTTCAAGATTAGGTTTTGGTTTTCCTGGTGGTCCTATTATTGACAAAATGTATCAAGATTACAAAGGCGACTTTGTAGCTTTAACCTTGCCTCATACTGATGGATTATATGATTTTAGTTTCAGTGGTCTTAAATCACAAGTTTTAAATCATTATAATAATGCTTTGCAAAGAAAGAAAGCTTTAAATAAAGAACAACTTGCTGTTAGTTTTCAAGAAACAGCAGTTACATATTTAATTAACAAAACTAAATTAGCATTAGATAATCATCCAGAAATTAAAACTTTAGTTTTAGCTGGAGGAGTTAGTGCCAATAGTGAGCTTAGAAAAAGATTTTTAAAATTAAGCACTAAAGCTATAGTGCCTAATTTAAAATATTCAACTGATAATGGTGCAATGATTGCTATGTGTGCTTATTGTAAGATTAAATGA", "aa": "MRILGIETSHDDTSIALLEDGKVLALKTISQIDIFKEFGGTIPEISSREHVKNINLIEKIFLQEFDLNTIDYIAYTQKPGLIGTLQIGFLFASALALALNKPLLPINHLEGHFYSAAIKQEIKYPALSLLVSGGHTQLMLVKSPFDIQIIGQTLDDAVGEAYDKVASRLGFGFPGGPIIDKMYQDYKGDFVALTLPHTDGLYDFSFSGLKSQVLNHYNNALQRKKALNKEQLAVSFQETAVTYLINKTKLALDNHPEIKTLVLAGGVSANSELRKRFLKLSTKAIVPNLKYSTDNGAMIAMCAYCKIK", "aa_hexdigest": "76797c6e264aceb77345a7d7f134f2ce", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_C4XEI8", "query_cov": 1.0, "subject_cov": 0.9903536977491961, "identity": 1.0, "score": 606.0, "evalue": 4.46e-219, "valid": true, "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_Q4A734", "go_ids": [ "GO:0002949" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A734", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A734" ], "product": "tRNA N6-adenosine threonylcarbamoyltransferase" }, "genes": [ "tsaD" ], "id": "OAIKOHHJFB_604", "locus": "GCA000209735_00578" }, { "type": "cds", "sequence": "contig_1", "start": 314308, "stop": 314487, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCCCATTCAATCTCTCCATTAACCCTTGATGAACATATTGAAAAAATATATGATGCATTTATAAAATTTATAAATGAAAAGTACAACAATGACACCACTACTTTTAGTGCTGAAAATTTCGCAAAAGAATTTAATGAAGTAAATGATGGTAATGGAGTTAAATTAGAGAATGTTTAG", "aa": "MAHSISPLTLDEHIEKIYDAFIKFINEKYNNDTTTFSAENFAKEFNEVNDGNGVKLENV", "aa_hexdigest": "469031b184769c8c4abd8857365a8739", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6767.3659000000025, "isoelectric_point": 4.562602424621583 }, "id": "OAIKOHHJFB_605", "locus": "GCA000209735_00579" }, { "type": "cds", "sequence": "contig_1", "start": 314701, "stop": 314847, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAAAACTTATCTTTAAAGAACCGCGTTATTTTGAAGATGCAACTCTAAGTGTAGATAATAATAACAAATATAAAATAAGTTTTGGAAATACCAAAATGCATGCTGCTGATTTTGAAAAAATAATTAATATTAATGATATTTGA", "aa": "MPKLIFKEPRYFEDATLSVDNNNKYKISFGNTKMHAADFEKIININDI", "aa_hexdigest": "57638aadb113e50bf9359a2750029b05", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5635.3845, "isoelectric_point": 6.533323860168457 }, "id": "OAIKOHHJFB_606", "locus": "GCA000209735_00580" }, { "type": "cds", "sequence": "contig_1", "start": 314998, "stop": 315336, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGATAATGAATTCTTGAATAAATCAATATTTGAATCTACTTTATCAAACGAAAACTTTGAATTTGAAGTTTCAAATCAAAATAATAAACCACAAATTACTTTAAAAATCAAGAAATTTAGACATTTTTCAAAAAAATATTTCAATTGGGACGAAATTGAAAACAATAATTACAACAATGTGTTATATGCAATCACAAAGAAATCAGAAATTATTTTTGATAATGTAGATAATGAATGTGAAACGTTGCTTGACTTAGCAATCAACGATTATATTGAAAAAAGAAAAAATGAAATACTTTTATACTCAGAACGTATGAAAACAACTGATTCATGA", "aa": "MFDNEFLNKSIFESTLSNENFEFEVSNQNNKPQITLKIKKFRHFSKKYFNWDEIENNNYNNVLYAITKKSEIIFDNVDNECETLLDLAINDYIEKRKNEILLYSERMKTTDS", "aa_hexdigest": "8d4c4933ad0dc1aac0b5485f225cf56c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13523.950899999982, "isoelectric_point": 4.929839134216308 }, "id": "OAIKOHHJFB_607", "locus": "GCA000209735_00581" }, { "type": "cds", "sequence": "contig_1", "start": 315658, "stop": 315930, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTAAGAAAAATCATTTGGCTAAAAATATTAGTAATACAGTACCAAATCTATCAAGTGAAACTAATATATCAACTTATGAAAAAAATGATGAAAAAATTATAAGAGTTGAAGATATTAAATTAAATGATACAGGAGCAGAAGATCCAAATACTCATGAAAAAGAATTTGAAAGATTTTTCTTGATTTCTTTAAATGAAAGAAAAATTCGTTCTAATGGTGAAAAAACTGGAATTAAAGAATTAAAATATGATGAAAAAATTACTAAATGA", "aa": "MIKKNHLAKNISNTVPNLSSETNISTYEKNDEKIIRVEDIKLNDTGAEDPNTHEKEFERFFLISLNERKIRSNGEKTGIKELKYDEKITK", "aa_hexdigest": "c4a771f85a435bd438e01046707582df", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10494.68619999999, "isoelectric_point": 6.744082832336426 }, "id": "OAIKOHHJFB_608", "locus": "GCA000209735_00582" }, { "type": "cds", "sequence": "contig_1", "start": 316786, "stop": 317580, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCGGATTTAAACAATGCCAATACTTTAAATATAGTTAAGGAATATTGTGGAATAAAAGATGAAAAAAATATAAATATTGTATTTCAAACTTTTATTAAAAAATACATTTCAAATTCAAAATTAGAATTCATAAGTCGTTTTGAAAATATACTTTCAAATGCGGATTACAACAAAAAAACATTTTATTATGATTCAGAAGTTGCAAAATTATCAGTTGATAAGAAATTCGATAAAATAACAAATTACTTTTACGGTACTAATTCAAATTTTTTTGATTCATTTATAAATGATAAATATACATTTAATATTTCATTAAAAAATATTGGTTTTAAAAAAGATAATACAACATATCTCGACATTAATAATTTAAATAAAGAAATAAGCGTAACATATGATATTGTAAACAAAATTTATGAATTTAATACAATAGGTTTTTTTGCTACAAACAATATCAACAAAATAGTTGATGAATTATTTAAAGAAAAAGATAATATCTTTAAATATCCTTTACAAGATTTAGATTATGAAAAATGTAGAAAAGAAATAATTGAAAAAATAAAAAATGATATTCAAAGTAAATTGACTAATGCTTATATTTCTGAAATTGATAATGAATGTGATTTATCAAAATTGAATATAAAAGCTGGTGTAAATTATCCTTCAGAATACAAAGATAAAAATATAGAAATTGTAACTCTTGAATCAAAAACATTAAAACCATCAGGTGTTTCTGACTTTGATAGTTTTAAAAAATATAACGAATTAAAAAATTATTTAAATAGTTCGATTTGA", "aa": "MPDLNNANTLNIVKEYCGIKDEKNINIVFQTFIKKYISNSKLEFISRFENILSNADYNKKTFYYDSEVAKLSVDKKFDKITNYFYGTNSNFFDSFINDKYTFNISLKNIGFKKDNTTYLDINNLNKEISVTYDIVNKIYEFNTIGFFATNNINKIVDELFKEKDNIFKYPLQDLDYEKCRKEIIEKIKNDIQSKLTNAYISEIDNECDLSKLNIKAGVNYPSEYKDKNIEIVTLESKTLKPSGVSDFDSFKKYNELKNYLNSSI", "aa_hexdigest": "8e6119a6635980c69c115b71b2737a3f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 30974.611400000045, "isoelectric_point": 5.829770851135254 }, "id": "OAIKOHHJFB_609", "locus": "GCA000209735_00583" }, { "type": "cds", "sequence": "contig_1", "start": 318601, "stop": 319125, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAACAATAAAAAAACAAACATTGATTATCCATTTTATTGTAGTGAACTATTAAAAAATTCTGATTTTAATAATTCGACAGTTTTTGGCAATGAATCAAATAAAGCTGAATTAAAAAATACCAACAATACTTTTAAAATTAGATATGATATTTCTCCAAGAATTAAGATTTTTGATATTTCAATCGATAAAACTAATAAAGAACATGCCGAAGAAGTTGAGTCATTATTAAAGAATAATAAATTAGATGACATTTTCAATAGAAAAATATTTTCAAATAATATTAAAAATGAATTATTACCTCCTGAAAAATTTAGTACTATTAAAGGTGAACCAGGTTGTGATGAGTTTATTGAATCTTACTCAGAAAATAAAAAATATAATACTAAACTAATTTCTTCTAATCCAAATACACCATTGATTAACTTATATTTCATTTCTGAAGACCAAAAAACTTTATACAAAGTACCTGAAAAAGTAGATAAAAATGACCTTGTTTATAATTTGTTTAAAGAATATTGA", "aa": "MENNKKTNIDYPFYCSELLKNSDFNNSTVFGNESNKAELKNTNNTFKIRYDISPRIKIFDISIDKTNKEHAEEVESLLKNNKLDDIFNRKIFSNNIKNELLPPEKFSTIKGEPGCDEFIESYSENKKYNTKLISSNPNTPLINLYFISEDQKTLYKVPEKVDKNDLVYNLFKEY", "aa_hexdigest": "9c710547700f4c05a9c03d0a7c309ff0", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20434.69460000001, "isoelectric_point": 5.627708244323729 }, "id": "OAIKOHHJFB_610", "locus": "GCA000209735_00584" }, { "type": "cds", "sequence": "contig_1", "start": 319186, "stop": 319314, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAGAATTGAGCTTGTTAAAGGTCAATGCAATTCAGAATATGATGTGGTTATTTTAAAAATAAACAACATTTATATAAGTTCAAATGATAAATCAATAGTTGAAAAAATAAAAAATGACATTTGA", "aa": "MPRIELVKGQCNSEYDVVILKINNIYISSNDKSIVEKIKNDI", "aa_hexdigest": "46fdcb4130c5689acf9d8b4fbef4e0ad", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4837.5699, "isoelectric_point": 6.057922554016113 }, "id": "OAIKOHHJFB_611", "locus": "GCA000209735_00585" }, { "type": "cds", "sequence": "contig_1", "start": 319720, "stop": 319827, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAAAAACAAAAGAAAAAAAGTTATAGCGACAGTGGCAACTGCACTCTCGTTAACAACACTTGGAGTAGTTAGTGCTGCTGCTTTTTACAATGTAAATAGCTGA", "aa": "MIKNKRKKVIATVATALSLTTLGVVSAAAFYNVNS", "aa_hexdigest": "9a5e590a97f90a0d09366438282a78dd", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3681.3502, "isoelectric_point": 10.582501411437985 }, "id": "OAIKOHHJFB_612", "locus": "GCA000209735_00586" }, { "type": "cds", "sequence": "contig_1", "start": 320179, "stop": 320283, "strand": "+", "frame": 1, "gene": "ydbC", "product": "YdbC family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4P6MMX1", "UniRef:UniRef90_UPI000FF2E50B" ], "nt": "ATGGCATACAAAAAACCAGAAATTACAAACAAGATTGTTCAACATGTTGGTGAAATTTCAACAACAAATAGTGGTTATAAAAAAGAACTCAATTTAGTTTCATGA", "aa": "MAYKKPEITNKIVQHVGEISTTNSGYKKELNLVS", "aa_hexdigest": "172154335c0cd7455d67729fa2d6f02c", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [ "ydbC" ], "truncated": "3-prime", "pseudogene": { "start": 320179, "stop": 320417, "observations": { "deletion": [ 320371 ], "mutation": [ 320281, 320317 ], "stop": [ 320281, 320317, 320395 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000FF2E50B", "query_cov": 1.0, "subject_cov": 0.43037974683544306, "identity": 1.0, "score": 70.5, "evalue": 3.25e-15, "gene_start": 1, "gene_end": 34, "reference_start": 1, "reference_end": 34, "reference_sequence": "MAYKKPEITNKIVQHVGEISTTNSGYKKELNLVSWNEGEAKYDIRDWSEDHLRSSKGITLSLEEFKTIKSFDWQRASKN" }, "paralog": false, "identity": 0.9493670886075949, "score": 139.0, "evalue": 6.8e-45, "description": "Internal stop codon at 320281, 320317, 320395. Frameshift due to deletion around 320371. Nonsense mutation around 320281, 320317.", "psc": { "uniref90_id": "UniRef90_UPI000FF2E50B", "product": "YdbC family protein", "uniref50_id": "UniRef50_A0A4P6MMX1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000FF2E50B", "UniRef:UniRef50_A0A4P6MMX1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A4P6MMX1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4P6MMX1" ], "product": "Transcriptional coactivator p15 (PC4) C-terminal domain-containing protein" } }, "id": "OAIKOHHJFB_613", "locus": "GCA000209735_00587" }, { "type": "cds", "sequence": "contig_1", "start": 320424, "stop": 321221, "strand": "+", "frame": 3, "gene": null, "product": "N-acetyltransferase domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354463.1", "SO:0001217", "UniParc:UPI0001E33029", "UniRef:UniRef100_C4XEJ2", "UniRef:UniRef50_C4XEJ2", "UniRef:UniRef90_C4XEJ2" ], "nt": "ATGATTTTAGCAACTAAAGATGATAAAGAAGTAATTCTTAAATTCTTAAATCAAGAGCCAATTTATAATTTACTAATTGTTGGCGACATTATGACTTATGGGGTTAGAACACCTTATCTTTTTACTTATATCATTAAAGAAGAAGAGCAAATTAAAGCAGTAATATTGATATTCAATAAAACAGTTTTAATTTATGATCCTGAATTTAAAGTAAGTCTAAAGGATTTAAAATCTTTAATTAAAAAACATTCACTTATTAATGTTAATTTAAGCGAGAAAATGTATCAACATTATCAAGATGAGTTTGAAAATAATGGAGATAAATACAAGGTTCATCGTCAAACTATAGCAATATTGGAAAATAGATACACTGGTGATACTTCACTAGCTAAAAAAGCTATTTTTACAGATTCAGAGCCAATAGTTCGTAGTAGATTTTTAATTGATGAATTTGTAGACTTTAGAGGGACTTATCAACAAGAAGTAAAATCTTATAAAAATGCTTTAATAAAAAAAGTTTCAACGCCTTTCATTATTAAAGACAAACAAAATCGAGTAGCATCACATGCTAATATATTGATATCTTCAGATAAAGCATCAATGATTGGCGGTGTGTTTACTTTAAAAGAATATCGACAAAAAGGTTATGCAATACAAGTAGTTGGCGCCTTATGCAACCATATTTTTGCCAACAAAAGAGTACCGATGCTTTTCTTTGATAACCCAGCAGCAGCACAACTATATCACAAAATAGGTTTTAAAGATTTTGGCAAGGTTTTTACAATTAAAATAAATTAA", "aa": "MILATKDDKEVILKFLNQEPIYNLLIVGDIMTYGVRTPYLFTYIIKEEEQIKAVILIFNKTVLIYDPEFKVSLKDLKSLIKKHSLINVNLSEKMYQHYQDEFENNGDKYKVHRQTIAILENRYTGDTSLAKKAIFTDSEPIVRSRFLIDEFVDFRGTYQQEVKSYKNALIKKVSTPFIIKDKQNRVASHANILISSDKASMIGGVFTLKEYRQKGYAIQVVGALCNHIFANKRVPMLFFDNPAAAQLYHKIGFKDFGKVFTIKIN", "aa_hexdigest": "811f442de7ab516ae9258b3f107138e7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E33029", "ncbi_nrp_id": "WP_013354463.1", "uniref100_id": "UniRef100_C4XEJ2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33029", "RefSeq:WP_013354463.1", "UniRef:UniRef100_C4XEJ2" ] }, "ips": { "uniref100_id": "UniRef100_C4XEJ2", "uniref90_id": "UniRef90_C4XEJ2" }, "psc": { "uniref90_id": "UniRef90_C4XEJ2", "product": "N-acetyltransferase domain-containing protein", "uniref50_id": "UniRef50_C4XEJ2" }, "pscc": { "uniref50_id": "UniRef50_C4XEJ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEJ2" ], "product": "N-acetyltransferase domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_614", "locus": "GCA000209735_00588" }, { "type": "cds", "sequence": "contig_1", "start": 321235, "stop": 321852, "strand": "+", "frame": 1, "gene": "ffh", "product": "signal recognition particle protein", "db_xrefs": [ "EC:3.6.5.4", "GO:0003924", "GO:0005048", "GO:0005525", "GO:0009306", "GO:0048501", "KEGG:K03106", "SO:0001217", "UniRef:UniRef50_A0A449BAE8", "UniRef:UniRef90_A0A3P8MDR8" ], "nt": "ATGCTTAATTTTTTAGAAAACAGAATTCAAAAGTCAATTCAAAAAATGGCTAGAAAAACTGTAGTTAATGAAGAAGACATTTTAGAAGTAACAAGAGACATAAAAATGGCTCTTTTAGAAGCTGACGTTAACTTAAAAATAGTTAAAGAATTTATTGTTAATGTTAAACAAAAAGCTTTAGAAAGCAAATTAGTTGGTTCACTTAATGCTAGTCAACAAATGATTAAAATTGTTCACAATGAACTTGTGGACATTTTAGGTGGCAAAGTCCAAGAAGTTAAAATTACGGCTCGTCCTTATATTATTATGATGGCAGGACTTCAAGGATCTGGTAAAACTACAGCTTGCGCTAAATTAGCTTATTACTTAAGAAAGAAAAACTTTGTAACAAAACCTTTAGTAGTAGCAGCTGATATTTATCGTCCAGCTGCTGTTCAACAATTAGTAACTTTAGCTAAAAGTATTCAAATTGATTACTTTGAAAAAGGCACTAATGTTAGTGCTCAAGATATTGTCAAAGAAGCATTGAAAGTCGCTGATGCACAAAAAAATGATTTAATAATCATCGATACTGCTGGTCGTTTGTCAATTGATGAGCCTCTTATGCAAGAACTTTGA", "aa": "MLNFLENRIQKSIQKMARKTVVNEEDILEVTRDIKMALLEADVNLKIVKEFIVNVKQKALESKLVGSLNASQQMIKIVHNELVDILGGKVQEVKITARPYIIMMAGLQGSGKTTACAKLAYYLRKKNFVTKPLVVAADIYRPAAVQQLVTLAKSIQIDYFEKGTNVSAQDIVKEALKVADAQKNDLIIIDTAGRLSIDEPLMQEL", "aa_hexdigest": "53b37c338906263ae741a9d209ed6956", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "ffh" ], "truncated": "3-prime", "pseudogene": { "start": 321217, "stop": 322155, "observations": { "mutation": [ 321850 ], "stop": [ 321850 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8MDR8", "query_cov": 1.0, "subject_cov": 0.4466230936819172, "identity": 1.0, "score": 383.0, "evalue": 2.28e-130, "gene_start": 1, "gene_end": 205, "reference_start": 7, "reference_end": 211, "reference_sequence": "MNKGDNMLNFLENRIQKSIQKMARKTVVNEEDILEVTRDIKMALLEADVNLKIVKEFIVNVKQKALESKLVGSLNASQQMIKIVHNELVDILGGKVQEVKITARPYIIMMAGLQGSGKTTACAKLAYYLRKKNFVTKPLVVAADIYRPAAVQQLVTLAKSIQIDYFEKGTNVSAQDIVKEALKVADAQKNDLIIIDTAGRLSIDEPLMQELWDLKKIANPSEIFFVADALSGQDILNVAETFNNKLKLTGTIITKLDSDARGGAALSLTKMLKLPIRFIGTGEKVSNLDLFYPDRMADRILGMGDVMSLIEKAEEVIDPKMANNMVAKILRGNFTLDDLMEQLTQIKKLGKMSKILKMIPGLARKIDEDKIAEAEMKMYNYEILISSMTPQERKNPKLLKNANRKARIIKGSGRSAQEYNRLLNDFDQLVKNMGEMAKKINAGNFSDIAKMGGFGGGAA" }, "paralog": false, "identity": 0.9935897435897436, "score": 587.0, "evalue": 4.27e-213, "description": "Internal stop codon at 321850. Nonsense mutation around 321850.", "psc": { "uniref90_id": "UniRef90_A0A3P8MDR8", "gene": "ffh", "product": "signal recognition particle protein", "ec_ids": [ "3.6.5.4" ], "uniref50_id": "UniRef50_A0A449BAE8", "kegg_orthology_id": "K03106", "go_ids": [ "GO:0003924", "GO:0005048", "GO:0005525", "GO:0009306", "GO:0048501" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8MDR8", "EC:3.6.5.4", "UniRef:UniRef50_A0A449BAE8", "KEGG:K03106", "GO:0003924", "GO:0005048", "GO:0005525", "GO:0009306", "GO:0048501" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449BAE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449BAE8" ], "product": "Signal recognition particle protein" } }, "id": "OAIKOHHJFB_615", "locus": "GCA000209735_00589" }, { "type": "cds", "sequence": "contig_1", "start": 321952, "stop": 322596, "strand": "+", "frame": 1, "gene": null, "product": "Signal recognition particle protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PFZ2" ], "nt": "TTGAAACTTACAGGTACTATTATTACTAAATTAGATTCAGATGCTCGTGGTGGAGCTGCATTAAGCTTAACCAAGATGTTAAAATTACCAATTAGATTTATTGGTACTGGTGAAAAAGTTTCAAACTTAGATCTTTTCTACCCTGATAGAATGGCTGATAGAATTTTAGGTATGGGTGATGTTATGAGCTTGATTGAAAAAGCTGAAGAAGTCATCGATCCTAAAATGGCTAACAATATGGTTGCTAAAATTTTAAGAGGTAATTTTACTCTAGATGATTTAATGGAACAATTAACTCAAATTAAAAAACTTGGCAAAATGAGCAAGATATTAAAAATGATTCCAGGACTTGCAAGAAAGATTGATGAAGATAAAATTGCTGAAGCAGAAATGAAAATGTATAACTATGAAATTTTGATTTCATCAATGACACCACAAGAAAGAAAAAATCCTAAATTACTTAAAAATGCTAATCGTAAAGCTAGAATTATTAAAGGTAGTGGACGCAGTGCTCAAGAATATAATCGTTTACTAAATGATTTTGATCAGTTAGTTAAAAACATGGGTGAAATGGCTAAAAAAATTAATGCAGGTAATTTTAGCGATATTGCTAAAATGGGAGGTTTTGGCGGAGGTGCGGCTTAA", "aa": "MKLTGTIITKLDSDARGGAALSLTKMLKLPIRFIGTGEKVSNLDLFYPDRMADRILGMGDVMSLIEKAEEVIDPKMANNMVAKILRGNFTLDDLMEQLTQIKKLGKMSKILKMIPGLARKIDEDKIAEAEMKMYNYEILISSMTPQERKNPKLLKNANRKARIIKGSGRSAQEYNRLLNDFDQLVKNMGEMAKKINAGNFSDIAKMGGFGGGAA", "aa_hexdigest": "3c1974398956a2d55a7827bd71c8d0d4", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PFZ2", "query_cov": 0.9719626168224299, "subject_cov": 0.9285714285714286, "identity": 0.635, "score": 249.0, "evalue": 4.86e-81, "valid": false, "product": "Signal recognition particle protein", "uniref50_id": "UniRef50_A0A3B0PFZ2" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PFZ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PFZ2" ], "product": "Signal recognition particle protein" }, "genes": [], "id": "OAIKOHHJFB_616", "locus": "GCA000209735_00590" }, { "type": "cds", "sequence": "contig_1", "start": 322923, "stop": 323087, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTATTAATTGCTCTTTCATTAATATTCAATAAACTCCATTATGTTAAATATTATGGCTTTTTAGCAATTATTTCTGGTTTATTGGCTTTAGTTAAAGTTGATTTTAACTTTACGCAAGAAGAAGGCAAGGATATAATATTTCCAATTGGAATTGATAATTGA", "aa": "MLLIALSLIFNKLHYVKYYGFLAIISGLLALVKVDFNFTQEEGKDIIFPIGIDN", "aa_hexdigest": "11aa76552efaa29230bbb8baa593fc89", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6133.240400000001, "isoelectric_point": 5.498115348815917 }, "id": "OAIKOHHJFB_617", "locus": "GCA000209735_00591" }, { "type": "cds", "sequence": "contig_1", "start": 324280, "stop": 324768, "strand": "+", "frame": 1, "gene": null, "product": "F0F1 ATP synthase subunit A", "db_xrefs": [ "EC:7.1.2.2", "SO:0001217", "UniRef:UniRef50_C4XEJ6", "UniRef:UniRef90_C4XEJ6" ], "nt": "ATGCCCCAAATGTTTTCCTTATTTATTTTGATATTAGTTATCTTTATTATTTCACTAACCACATTTATCTTAGTAAAGAAAACATCACGTCTAGATAAAGCGCCCATTGGATTCTTGATAGTTATGGAAAGTTATGTGACATTCTTGGATTCAAGTTTTGAAGAAAACACTGAAGGTGCTTTACCAAAAGCAAAATTTTACATTTTTGCATTGTTTACATTTTTATTGCTAGGTAATTTGTTAGGTCTTTTGGGCCTTAAACCAATTGCAACTAGTTATTCAATAACATTTGCTTTAGCATTCATTTCATTTGCTGGAATTTATGTTGTTGGACTAATTTATCAAAAATTCAGATTCTTTAGTAGATTCTTAAGTCCAATTGAACTTGTAGGTCAATTTGCACCTTTAATTTCACTTGGCTTTCGGATTTTTGGTAACATCACTGGTGGTGGAGTTATTGTGTTCTTACTTTATAGTTTCACAGGATGA", "aa": "MPQMFSLFILILVIFIISLTTFILVKKTSRLDKAPIGFLIVMESYVTFLDSSFEENTEGALPKAKFYIFALFTFLLLGNLLGLLGLKPIATSYSITFALAFISFAGIYVVGLIYQKFRFFSRFLSPIELVGQFAPLISLGFRIFGNITGGGVIVFLLYSFTG", "aa_hexdigest": "5f677a454876725bd33c4273e99f211d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 324199, "stop": 325038, "observations": { "mutation": [ 324268, 324274, 324766, 324772, 324802, 324907, 325012 ], "stop": [ 324268, 324274, 324766, 324772, 324802, 324907, 325012 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEJ6", "query_cov": 1.0, "subject_cov": 0.5806451612903226, "identity": 1.0, "score": 299.0, "evalue": 9.62e-101, "gene_start": 1, "gene_end": 162, "reference_start": 28, "reference_end": 189, "reference_sequence": "MKKTKKFKITRKGGQVEKLTQNLWKWNMPQMFSLFILILVIFIISLTTFILVKKTSRLDKAPIGFLIVMESYVTFLDSSFEENTEGALPKAKFYIFALFTFLLLGNLLGLLGLKPIATSYSITFALAFISFAGIYVVGLIYQKFRFFSRFLSPIELVGQFAPLISLGFRIFGNITGGGVIVFLLYSFTGWIWTQIPGLLGEWFFIGSLITPFLHMYFDMFGAIIQALVFCTLTTVYWSKEAVTNENKKQQDAQEKAESEKSVANVENKAVAWSNSSSVY" }, "paralog": false, "identity": 0.9713261648745519, "score": 512.0, "evalue": 3.86e-187, "description": "Internal stop codon at 324268, 324274, 324766, 324772, 324802, 324907, 325012. Nonsense mutation around 324268, 324274, 324766, 324772, 324802, 324907, 325012.", "psc": { "uniref90_id": "UniRef90_C4XEJ6", "product": "F0F1 ATP synthase subunit A", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_C4XEJ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEJ6", "EC:7.1.2.2", "UniRef:UniRef50_C4XEJ6" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEJ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEJ6" ], "product": "ATP synthase subunit a" } }, "id": "OAIKOHHJFB_618", "locus": "GCA000209735_00592" }, { "type": "cds", "sequence": "contig_1", "start": 325058, "stop": 325294, "strand": "+", "frame": 2, "gene": "atpE", "product": "ATP synthase F0 subunit C", "db_xrefs": [ "EC:7.1.2.2", "GO:0015078", "GO:0015986", "GO:0045263", "RefSeq:WP_013354468.1", "SO:0001217", "UniParc:UPI0001E3302E", "UniRef:UniRef100_C4XEJ7", "UniRef:UniRef50_C4XEJ7", "UniRef:UniRef90_C4XEJ7" ], "nt": "ATGACTCAAGGTGGAGGTTTAGTTGCAATCGGTATTGGTATTGCAATGTTAGGTAGTTTTGGAACAGGTTTAGGACAAGGCTTAGCTGCTGGTAAAGCTACTGAAGCTGTTGGACGTAATCCTGAAGCTGCAGCTAAAATTCGTTCAATGTTACTTATTGGACAAGGTGTTGCAGAATCTTCAGCTATCTATTGTTTAGTTGTTGCCTTTATTCTAGCTTTTGCTTTTACAAAATAA", "aa": "MTQGGGLVAIGIGIAMLGSFGTGLGQGLAAGKATEAVGRNPEAAAKIRSMLLIGQGVAESSAIYCLVVAFILAFAFTK", "aa_hexdigest": "c899121cf9fca3778600cf0a4ba77d9a", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001E3302E", "ncbi_nrp_id": "WP_013354468.1", "uniref100_id": "UniRef100_C4XEJ7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3302E", "RefSeq:WP_013354468.1", "UniRef:UniRef100_C4XEJ7" ] }, "ips": { "uniref100_id": "UniRef100_C4XEJ7", "uniref90_id": "UniRef90_C4XEJ7" }, "psc": { "uniref90_id": "UniRef90_C4XEJ7", "gene": "atpE", "product": "ATP synthase F0 subunit C", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_C4XEJ7", "go_ids": [ "GO:0015078", "GO:0015986", "GO:0045263" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEJ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEJ7" ], "product": "ATP synthase subunit c" }, "genes": [ "atpE" ], "id": "OAIKOHHJFB_619", "locus": "GCA000209735_00593" }, { "type": "cds", "sequence": "contig_1", "start": 325302, "stop": 325826, "strand": "+", "frame": 3, "gene": "atpF", "product": "F0F1 ATP synthase subunit B", "db_xrefs": [ "EC:7.1.2.2", "GO:0015078", "GO:0015986", "GO:0045263", "RefSeq:WP_013354469.1", "SO:0001217", "UniParc:UPI0001E3302F", "UniRef:UniRef100_C4XEJ8", "UniRef:UniRef50_C4XEJ8", "UniRef:UniRef90_C4XEJ8" ], "nt": "ATGCACACAATCAATAAATCTTTAGGAACAAAAATTGAGGAAGAATTAAACGGTTTATTTCCTAGTGTTCCGATGCTTGTGGCAACTTTAGTTGCTCTTGCAATTATTATTTTGATTCTTTTCTTCTTATTATATAAACCAATTAAAAAGTCTATAAAAGCAAGACAAGATTATATTCAAAGTAATATTGATCAAGCTAAAGCAACTAATGACTTGTCAAAAAACAAGTTAAAAGAAGCAAATGACAAATTGATTCTAGCTCATGAAGAAGCTAATAACTTAGTTAAAAACGCTAAAATTAGAGCAGAAAAAGTTATTATTTCTTATACAGCAAAAGCTAAGATGGAATCAAAAAGAATAATAGAAGAAGCAGAATTAGATATTGAACAACAAAAAGATTCTTTAATGGAAGATTCAAAAAATCAAATTGCCAAAGCAGCTACAGAATTGTCAAGACAAATACTCAAAAAAGAAGTTTCAAAGAAAACTGAAAGTGAAATTATTGACAAGTTTCTTGAATCTTAA", "aa": "MHTINKSLGTKIEEELNGLFPSVPMLVATLVALAIIILILFFLLYKPIKKSIKARQDYIQSNIDQAKATNDLSKNKLKEANDKLILAHEEANNLVKNAKIRAEKVIISYTAKAKMESKRIIEEAELDIEQQKDSLMEDSKNQIAKAATELSRQILKKEVSKKTESEIIDKFLES", "aa_hexdigest": "36b825b4af6c6c4ced67b7de4879d183", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E3302F", "ncbi_nrp_id": "WP_013354469.1", "uniref100_id": "UniRef100_C4XEJ8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3302F", "RefSeq:WP_013354469.1", "UniRef:UniRef100_C4XEJ8" ] }, "ips": { "uniref100_id": "UniRef100_C4XEJ8", "uniref90_id": "UniRef90_C4XEJ8" }, "psc": { "uniref90_id": "UniRef90_C4XEJ8", "gene": "atpF", "product": "F0F1 ATP synthase subunit B", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_C4XEJ8", "go_ids": [ "GO:0015078", "GO:0015986", "GO:0045263" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEJ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEJ8" ], "product": "ATP synthase subunit b" }, "genes": [ "atpF" ], "id": "OAIKOHHJFB_620", "locus": "GCA000209735_00594" }, { "type": "cds", "sequence": "contig_1", "start": 325868, "stop": 326425, "strand": "+", "frame": 2, "gene": "atpH", "product": "ATP synthase F1 subunit delta", "db_xrefs": [ "EC:7.1.2.2", "GO:0015986", "GO:0045261", "GO:0046933", "RefSeq:WP_013354470.1", "SO:0001217", "UniParc:UPI0001A8C1A4", "UniRef:UniRef100_C4XEJ9", "UniRef:UniRef50_C4XEJ9", "UniRef:UniRef90_C4XEJ9" ], "nt": "ATGTACTCAAAAGCTAATCCAGAAGGATATGCTTTGGCTCTTTTTGAATTGACAAAAGAAGAAAAAGATATGAAAGCTATTAATGAAGTTGTTGTCTCTCTTTATAACACAGTTGTAGAAAGTGATAAACTCGTTATGATATTAAATGATCAAGCTTTAGAAAAAAATAAAAAGTTTGATTACATTGACAAAATTTTTAAAAACATTTCAAGTAGTAAACTAATAGTTAACTTTTTAAAAGTTTTAGTAGAACGCAATGCAGCTACTCTTTTAAGAAGATCTCTTGAACATTATTTAAAGCTCTCAAATGAATTTTTAAACATTCGATTTGCTTTAATTACAAGTGCTTTTGAAATTAGTGAAGACAAACTTAAAAAAATTAAACAAAAACTTGAAAAGAAATATAACACTGAAATAATTCTTAAACATCAGATAGATGAAAGCTTAATTTCAGGTTTCAAAATTAAAATGGATAGTTTAGTTATTGAGCAAAACTTTAAAAAAGATTTACAAGAACTAAATACTATTCTTACTAAAAAAGGAGGTTTAGATGGCTAG", "aa": "MYSKANPEGYALALFELTKEEKDMKAINEVVVSLYNTVVESDKLVMILNDQALEKNKKFDYIDKIFKNISSSKLIVNFLKVLVERNAATLLRRSLEHYLKLSNEFLNIRFALITSAFEISEDKLKKIKQKLEKKYNTEIILKHQIDESLISGFKIKMDSLVIEQNFKKDLQELNTILTKKGGLDG", "aa_hexdigest": "cfc6a7b40b325693d6bb4832d6c95216", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C1A4", "ncbi_nrp_id": "WP_013354470.1", "uniref100_id": "UniRef100_C4XEJ9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C1A4", "RefSeq:WP_013354470.1", "UniRef:UniRef100_C4XEJ9" ] }, "ips": { "uniref100_id": "UniRef100_C4XEJ9", "uniref90_id": "UniRef90_C4XEJ9" }, "psc": { "uniref90_id": "UniRef90_C4XEJ9", "gene": "atpH", "product": "ATP synthase F1 subunit delta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_C4XEJ9", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEJ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEJ9" ], "product": "ATP synthase subunit delta" }, "genes": [ "atpH" ], "id": "OAIKOHHJFB_621", "locus": "GCA000209735_00595" }, { "type": "cds", "sequence": "contig_1", "start": 326418, "stop": 327164, "strand": "+", "frame": 3, "gene": null, "product": "ATP synthase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9E1" ], "nt": "ATGGCTAGTAGAACAGACGATATTAGTGCAATAATTAAAGATCGAATTCGCAATTTCGACTCTAAAGTTGACTACTCAGAAGTCGGAACTATTATTACTATTGGTGATGGTATCGCCTTAGTTAAAGGTCTTGACAATGCTAAAAACGGTGAAATTATTTTATTCAAAAATAATATCTATGGGTTAGTTTTAAACCTTGAAGAAGAAGTAGTTGGAGTTACCATTTTTGGTAATGCTAACTCACTTTCAGAAGGTGACACTTGCAAAAGAAGTGGTGAAGTTATCTCAATTCCAGTTGGTGACAAACTTCTTGGCCGTGTAGTTAATGCCTTAGGTCAACCAATTGATGGCAAAGGCAAAATTAATACTACTGTTAAAAAAGAAATCTTTAAAGTTGCTCCTGGTGTTATGACACGTCAAGAAGTTAACCAACCTCTTGAAACTGGTATTATTGCAATTGACTCAATGATTCCTATTGGCAAAGGTCAACGTGAATTAATCATTGGTGACCGTCAAACTGGTAAAACTGCTATTGCAATTGATACAATCATTAACCAAAAAGGAAAAAATGTTAACTGTATTTATGTGGCTATTGGACAAAAAAATTCAACAGTTGCTCAAATAGTTAAAAAACTTGAAGACACTGGCGCTATGGAATATACAACTGTTGTAGTTTCTGGAGCTAGTGAACTTGCGCCTCAACAATATATTGCACCTTATTCAGGCGTTACTATTGCTGAAGAATGA", "aa": "MASRTDDISAIIKDRIRNFDSKVDYSEVGTIITIGDGIALVKGLDNAKNGEIILFKNNIYGLVLNLEEEVVGVTIFGNANSLSEGDTCKRSGEVISIPVGDKLLGRVVNALGQPIDGKGKINTTVKKEIFKVAPGVMTRQEVNQPLETGIIAIDSMIPIGKGQRELIIGDRQTGKTAIAIDTIINQKGKNVNCIYVAIGQKNSTVAQIVKKLEDTGAMEYTTVVVSGASELAPQQYIAPYSGVTIAEE", "aa_hexdigest": "af621f36fcb4a40cce4bae0cd82dd14c", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_A0A223M9E1", "query_cov": 0.967741935483871, "subject_cov": 0.9411764705882353, "identity": 0.604, "score": 302.0, "evalue": 1.09e-100, "valid": false, "product": "ATP synthase subunit alpha", "uniref50_id": "UniRef50_A0A223M9E1" }, "pscc": { "uniref50_id": "UniRef50_A0A223M9E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9E1" ], "product": "ATP synthase subunit alpha" }, "genes": [], "id": "OAIKOHHJFB_622", "locus": "GCA000209735_00596" }, { "type": "cds", "sequence": "contig_1", "start": 327165, "stop": 328004, "strand": "+", "frame": 3, "gene": null, "product": "ATP synthase alpha chain", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q1RKD9" ], "nt": "ATGACCAAAGGTAAAGACTGCTTAATTGTTTATGATGACTTATCTAAACATGCTATAGCTTATAGAACACTTTCGCTTCTTCTTCGTCGTCCACCTGGACGTGAAGCCTACCCCGGGGATGTTTTCTATCTTCACTCTCAACTTTTAGAAAGAGCTGCTAGAGTTACAAAAGAATATGGTGGAGGTTCAATTACTGCTTTACCTATTATTGAAACTCAACAAGGTGATATTTCAGCTTATATTCCAACTAATGTTATTTCTATAACAGATGGTCAAATTTTTACTAAAGAAAACTTATTTAATTCAGGCCAAAGACCTGCTGTTGATGTTGGTTTTAGTGTTAGCCGTGTTGGTAGTGCTGCTCAAATTAAAGCTATGAAAAAAGTAGCTGGTTCACTTAAACTAGAATTAGCTCAATATAATGAAATGTTAGCCTTTGCTCAATTTGGATCTGATCTTGATGATTCAACTAAAGCAATCTTGCAACATGGTGCTAAAGTTTATGAAATCTTAAAGCAAGAACAATATGCACCTATTAGCCAAATTGCTCAAGTAGCTATTTTGCTCGGGGTTAAAGAAAGAATAATAAATCCTCTTCCAGCTAAATACATAGCTCTTTATAGAGAAGAAGTAATTAAATACATCGAAAGTGATCCTGAAGGTGTTTCAATTGCTCTAGAAATTCATAACAATAATAATGAATTTAGTGATGTTCATTTTGAAACAATAACCAAAGCAATTTTAAAAATAGTAAAAAGAATTATTGCTTCAATTCCTAATTATGATCCAAGTGATTATTTGCCAATGCCTGAAAAATACAGTGAAAAGAGTAAAAAATAA", "aa": "MTKGKDCLIVYDDLSKHAIAYRTLSLLLRRPPGREAYPGDVFYLHSQLLERAARVTKEYGGGSITALPIIETQQGDISAYIPTNVISITDGQIFTKENLFNSGQRPAVDVGFSVSRVGSAAQIKAMKKVAGSLKLELAQYNEMLAFAQFGSDLDDSTKAILQHGAKVYEILKQEQYAPISQIAQVAILLGVKERIINPLPAKYIALYREEVIKYIESDPEGVSIALEIHNNNNEFSDVHFETITKAILKIVKRIIASIPNYDPSDYLPMPEKYSEKSKK", "aa_hexdigest": "148b28ee401b2079b5aa7a475d0be7d3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q1RKD9", "query_cov": 0.8853046594982079, "subject_cov": 0.8487972508591065, "identity": 0.5870000000000001, "score": 277.0, "evalue": 5.93e-90, "valid": false, "gene": "atpA", "product": "F0F1 ATP synthase subunit alpha", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q1RKD9", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_Q1RKD9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q1RKD9" ], "product": "ATP synthase alpha chain" }, "genes": [], "id": "OAIKOHHJFB_623", "locus": "GCA000209735_00597" }, { "type": "cds", "sequence": "contig_1", "start": 328004, "stop": 328876, "strand": "+", "frame": 2, "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "db_xrefs": [ "EC:7.1.2.2", "GO:0015986", "GO:0045261", "GO:0046933", "KEGG:K02115", "RefSeq:WP_013354472.1", "SO:0001217", "UniParc:UPI0001E33031", "UniRef:UniRef100_C4XEK1", "UniRef:UniRef50_F9UJQ6", "UniRef:UniRef90_C4XEK1" ], "nt": "ATGGCTGGTACTCAAGCAATTAAAAGTAGAATTTCTACTGTTGAATCTATTCGCAAAATAACTCATGCTATGGAACTAGTTGCTGCTAGCAAACTTAGAAGAGCAAGAATTGAATTCGAAAATGTTAAAGCTTATAATGAAGTAATTAAAGAAACTTTTCGTTCAATTTTAAATCATTTAAGCCCAACAGAAATTAAACATTTGTTTCCAATTCATAACATTGAAAGCAGAATGTTTATCATTGTAACTAGCGATTTAGGATTAGCTGGAAGCTATAATTCAAACATTATAAAATTTGCTAAAAACACTATAAAAAAGACAGATAAGGTAATTTTAATTGGCCGTAAAGCTATTAGTTCCTTAGCTTCTTTATATGAAAATCAAATTGTCACACAATTTAACTGTGATAGCAGTGAAAAAGACTACAACATCGTTCACGACATTATGACAATTGTATTTGAACTTTACAATCGAAAACAAGTAAATAGTATTAATGTAATTTATAACAAATACATGAATAACCTTGTTCAACAAGAAACTTGCGATCAAGTTTTTCCTTTTAAATTTGATGAAGATAAAAAACAAAAACACGCTGAAAAACACAAGGAATCACTTAATTCAATTATCGAATTTGAACCAAGTGCACAACAAGTTTTAAGCGAATCAATCCCACTTTTTATTGACGCTGAATTATATTTAGCTTACACTTCAGCCAAACTCAGTGAAATGGCTGCAAGAAGAAGTGCTATGGAATCAGCAACTGATAATGCTGATGAATTAATTGACAACTTAAATCTTGAATTCAACAGAAAACGTCAATCATCTATTACTCAAGAATTAAATGAAATAGTTGCTGGAGCTGACGCAGTATAG", "aa": "MAGTQAIKSRISTVESIRKITHAMELVAASKLRRARIEFENVKAYNEVIKETFRSILNHLSPTEIKHLFPIHNIESRMFIIVTSDLGLAGSYNSNIIKFAKNTIKKTDKVILIGRKAISSLASLYENQIVTQFNCDSSEKDYNIVHDIMTIVFELYNRKQVNSINVIYNKYMNNLVQQETCDQVFPFKFDEDKKQKHAEKHKESLNSIIEFEPSAQQVLSESIPLFIDAELYLAYTSAKLSEMAARRSAMESATDNADELIDNLNLEFNRKRQSSITQELNEIVAGADAV", "aa_hexdigest": "bf8dd84f316fdf6e87e135e633de846f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001E33031", "ncbi_nrp_id": "WP_013354472.1", "uniref100_id": "UniRef100_C4XEK1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33031", "RefSeq:WP_013354472.1", "UniRef:UniRef100_C4XEK1" ] }, "ips": { "uniref100_id": "UniRef100_C4XEK1", "uniref90_id": "UniRef90_C4XEK1" }, "psc": { "uniref90_id": "UniRef90_C4XEK1", "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_F9UJQ6", "kegg_orthology_id": "K02115", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_F9UJQ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_F9UJQ6" ], "product": "ATP synthase gamma chain" }, "genes": [ "atpG" ], "id": "OAIKOHHJFB_624", "locus": "GCA000209735_00598" }, { "type": "cds", "sequence": "contig_1", "start": 328886, "stop": 330394, "strand": "+", "frame": 2, "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "db_xrefs": [ "EC:7.1.2.2", "GO:0015986", "GO:0045261", "GO:0046933", "RefSeq:WP_013354473.1", "SO:0001217", "UniParc:UPI0001A8C1A7", "UniRef:UniRef100_C4XEK2", "UniRef:UniRef50_Q1AVH9", "UniRef:UniRef90_C4XEK2" ], "nt": "ATGGCAGTAAAGAAAACTACAACTAAAAAAGTTACTAGTGCTAAAAAATCAACTACTAAAGTCACAATAACTAAAAAACTAACTGAAAAAATTAATAAAAACAATGTTGGTAAAATAATCCAAGTTTTAGGACCTGTAGTCGATGTTAAATTTAACAAAGGCTTTTTACCTTCTTTATTAAATGCACTTAAAGTTTATTCAAATGATGAAGTTTATACTTTAGAAGTAGCTCAACATATCGGCGATGATACTGTTAGAACTATTTCAATGGTTTCAACTAATGGACTTGCAAGAGGACTTGATGTTATTGACACAGGAGCTCCCATTAGTGTTCCTGTTGGAGATAAAGTTTTAGGTCGTATGTTTGACGTTTTAGGTCAACCTATTGACTTACAAGAAGCTCCTAAAGCTCCACTTATGCCAATTCATGCATCTGCTCCTAGCTATGAAGAACAAAAAACAGTTAGTGAAGTGTTAGAAACTGGTATTAAAGTTATTGACCTTTTAATTCCATATGCAAAAGGTGGAAAGATCGGACTTTTTGGTGGTGCTGGTGTTGGAAAAACAGTTTTAGTTCAAGAATTAATTAATAATATTGCTACAGAACATGGTGGACTTTCAGTTTTCGCTGGTGTTGGTGAAAGAACTCGTGAAGGTAATGACCTTTACCATGAAATGAAAGCAGCTGGTGTTTTAGATAAAACTGCATTAGTCTTTGGACAAATGAACGAACCTCCTGGAGCACGTATGCGTGTTGCTTTAACTGGTTTAACAATGGCTGAATATTTTAGAGATAAAGAAAACCAAGATGTGCTTTTATTTATTGACAACATATTCCGTTTCACTCAAGCTGGTAGTGAAGTTTCAGCCCTTTTAGGTCGTATGCCTTCAGCTGTTGGTTACCAACCAACACTTGCTACTGAAATGGGTCAACTACAAGAAAGAATTACTTCAACACGTCGTGGATCAATTACTTCAGTGCAAGCGGTTTATGTTCCTGCCGATGACTTAACTGACCCTGCTCCTGCAACAACATTTAGCCACCTTGATGCTAAAACTGTTTTGGATAGAAATATTGCTGCTTTAGGTATTTATCCAGCAGTTGATCCTCTTGAATCAAACTCAAGATTATTAGATCCTCTTGTAGTTGGTCAAGAACACTATGAAGTAGCTCATGGAGTTGTTAACATTCTTCAAAGATTTAAAGAACTTCAAGACATTATTGCAATTCTTGGTATGGGTGAATTAAGTGAAGAAGATAAATTACTTGTTGCTCGTGCTCGTAGAATTAGAAATTTCCTTTCGCAACCATTTAATGTTGCTGAAAAATTTTCAGGTAAAAAAGGTAAATACGTTCCTATATCAGATACTATTAGAAGCTTCAAAGATATTCTCGAAGGTAAATACGATGATTGCCCTGAAGAATTCTTCTTATATGTTGGCTCAATCGATGAAGTTGAAGCTAAATACAAAGAATATTTAGCTAAAAAAGAAGCAAATAAAAATTAA", "aa": "MAVKKTTTKKVTSAKKSTTKVTITKKLTEKINKNNVGKIIQVLGPVVDVKFNKGFLPSLLNALKVYSNDEVYTLEVAQHIGDDTVRTISMVSTNGLARGLDVIDTGAPISVPVGDKVLGRMFDVLGQPIDLQEAPKAPLMPIHASAPSYEEQKTVSEVLETGIKVIDLLIPYAKGGKIGLFGGAGVGKTVLVQELINNIATEHGGLSVFAGVGERTREGNDLYHEMKAAGVLDKTALVFGQMNEPPGARMRVALTGLTMAEYFRDKENQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGQLQERITSTRRGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLDRNIAALGIYPAVDPLESNSRLLDPLVVGQEHYEVAHGVVNILQRFKELQDIIAILGMGELSEEDKLLVARARRIRNFLSQPFNVAEKFSGKKGKYVPISDTIRSFKDILEGKYDDCPEEFFLYVGSIDEVEAKYKEYLAKKEANKN", "aa_hexdigest": "8a9106edcfa70595edde033d65814494", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001A8C1A7", "ncbi_nrp_id": "WP_013354473.1", "uniref100_id": "UniRef100_C4XEK2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C1A7", "RefSeq:WP_013354473.1", "UniRef:UniRef100_C4XEK2" ] }, "ips": { "uniref100_id": "UniRef100_C4XEK2", "uniref90_id": "UniRef90_C4XEK2" }, "psc": { "uniref90_id": "UniRef90_C4XEK2", "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q1AVH9", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_Q1AVH9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q1AVH9" ], "product": "ATP synthase subunit beta" }, "genes": [ "atpD" ], "id": "OAIKOHHJFB_625", "locus": "GCA000209735_00599" }, { "type": "cds", "sequence": "contig_1", "start": 330438, "stop": 330863, "strand": "+", "frame": 3, "gene": null, "product": "ATP synthase epsilon chain", "db_xrefs": [ "RefSeq:WP_013354474.1", "SO:0001217", "UniParc:UPI0001A8C1A8", "UniRef:UniRef100_C4XEK3", "UniRef:UniRef50_A0A3P8MDS1", "UniRef:UniRef90_C4XEK3" ], "nt": "ATGTTGAATAAAAAAACAACACATTTAAAGATAACAACATTGGAAGAAATCTTTTTCGAAGGTGATGTTCGAAGTGTAACTCTTAGAACTAAAACTGGTGGTGCAATTTGCTTGCAACCAAGCCGTACTCCTTTCTTTAGTACAATAGATATTTGCGAATTAACAATTAATAGTCCTAAAGACAATGACTTTAGAGTTTGTTCAATTGGCGGTGGCTTAGTTTATGCTGATGCAACCAACATTAATATCATTACTGATGACATTATTTTTGGTAAAAATATTGACATTAAACGTGCTAAAATCGATCGTGATTTTGCTATGGCTCAACTTGAAAAATTTCGTGATACAAAAGAAGAAGTTCAATATGAAATTAAGTTAAGAAAAGCTTTAAACAGGATTGATGTTTATAATCGTAATATCAAATAA", "aa": "MLNKKTTHLKITTLEEIFFEGDVRSVTLRTKTGGAICLQPSRTPFFSTIDICELTINSPKDNDFRVCSIGGGLVYADATNINIITDDIIFGKNIDIKRAKIDRDFAMAQLEKFRDTKEEVQYEIKLRKALNRIDVYNRNIK", "aa_hexdigest": "a1ab310e0a3c7964d2ecff8d80619e81", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C1A8", "ncbi_nrp_id": "WP_013354474.1", "uniref100_id": "UniRef100_C4XEK3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C1A8", "RefSeq:WP_013354474.1", "UniRef:UniRef100_C4XEK3" ] }, "ips": { "uniref100_id": "UniRef100_C4XEK3", "uniref90_id": "UniRef90_C4XEK3" }, "psc": { "uniref90_id": "UniRef90_C4XEK3", "product": "ATP synthase epsilon chain", "uniref50_id": "UniRef50_A0A3P8MDS1" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MDS1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDS1" ], "product": "ATP synthase epsilon chain" }, "genes": [], "id": "OAIKOHHJFB_626", "locus": "GCA000209735_00600" }, { "type": "tRNA", "sequence": "contig_1", "start": 330968, "stop": 331041, "strand": "+", "gene": "trnG", "product": "tRNA-Gly(tcc)", "amino_acid": "Gly", "anti_codon": "tcc", "pseudogene": true, "score": 44.9, "nt": "GCAGATGTAGTTCAATGGTAGAGCTTTAGCCTTCCAAGTTGACTATGTGGGTTCGATTCCCGACATCCGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 331000, 331002 ], "id": "OAIKOHHJFB_3", "locus": "GCA000209735_00601" }, { "type": "cds", "sequence": "contig_1", "start": 331824, "stop": 331916, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAGAAATAATAATTTAAATAACTATATGGAATTAGTTACTCTTGGAAAAGAATATGAAGATCGTTCAAATAGAGCCAATTTAGCCTGA", "aa": "MNRNNNLNNYMELVTLGKEYEDRSNRANLA", "aa_hexdigest": "4fce2eaae5b6407c283ff1ba7c0d9ef4", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3557.8830000000007, "isoelectric_point": 6.013872337341308 }, "id": "OAIKOHHJFB_627", "locus": "GCA000209735_00602" }, { "type": "cds", "sequence": "contig_1", "start": 332205, "stop": 332492, "strand": "-", "frame": 3, "gene": null, "product": "Smr domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354476.1", "SO:0001217", "UniParc:UPI0001E33033", "UniRef:UniRef100_C4XEK6", "UniRef:UniRef50_C4XEK6", "UniRef:UniRef90_C4XEK6" ], "nt": "ATGAGTAGAAAAAATAAGAGAAACTATAAAAAAATAATCAATTATGATAAAGCAGAATCAAATGTCATCGACTTACATGGGTTAAATATTTATGAAGCAGTAGCAGAAATTCAATTTGCAATTGCTAATGCTAAAGATAATTCAGAATTTAGCAATAATTACATATTAGCCATTATCGGCAAAGGAACTGAAACTATAAAAGCATATATTGAAGAATTTTTAAGAGATTCAAACTATGAATATATGTTCAATGAGGACTATACCACTATCAAAATATATATTGCTTAG", "aa": "MSRKNKRNYKKIINYDKAESNVIDLHGLNIYEAVAEIQFAIANAKDNSEFSNNYILAIIGKGTETIKAYIEEFLRDSNYEYMFNEDYTTIKIYIA", "aa_hexdigest": "ffc184f34b9af43a1aa7957a0ec31ef5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E33033", "ncbi_nrp_id": "WP_013354476.1", "uniref100_id": "UniRef100_C4XEK6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33033", "RefSeq:WP_013354476.1", "UniRef:UniRef100_C4XEK6" ] }, "ips": { "uniref100_id": "UniRef100_C4XEK6", "uniref90_id": "UniRef90_C4XEK6" }, "psc": { "uniref90_id": "UniRef90_C4XEK6", "product": "Smr domain-containing protein", "uniref50_id": "UniRef50_C4XEK6" }, "pscc": { "uniref50_id": "UniRef50_C4XEK6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEK6" ], "product": "Smr domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_628", "locus": "GCA000209735_00603" }, { "type": "cds", "sequence": "contig_1", "start": 332846, "stop": 333127, "strand": "+", "frame": 2, "gene": null, "product": "Transposase", "db_xrefs": [ "RefSeq:WP_013354477.1", "SO:0001217", "UniParc:UPI0001E33034", "UniRef:UniRef100_UPI0001E33034", "UniRef:UniRef50_C4XEK7", "UniRef:UniRef90_C4XEK7" ], "nt": "TTGTATAATGAACCCCAAATTCCGGACTTTTTGGAAAGGGGTTCATTTTTATGCAATTCAAATTTAAAAAAGAAAAAAGGAAGAAAATCTAAAATAAATTTAGATGCTCAAAAGACAACAAATTTTAAAATCAATATTGAAAACAAAATAGAAGATAAAGATTTACTAATTAAACAATTAAAAGAAGAAAATAAAATACTTAAATTGGAGAATGCGATAGCAAAAAAAATGAGCGCCTTGGTTCAATTGAAAGACTCACTAACAAAGAAAAATTCCAAATAA", "aa": "MYNEPQIPDFLERGSFLCNSNLKKKKGRKSKINLDAQKTTNFKINIENKIEDKDLLIKQLKEENKILKLENAIAKKMSALVQLKDSLTKKNSK", "aa_hexdigest": "1d2463fd289f62c22a2d77c16b95063d", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E33034", "ncbi_nrp_id": "WP_013354477.1", "uniref100_id": "UniRef100_UPI0001E33034", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33034", "RefSeq:WP_013354477.1", "UniRef:UniRef100_UPI0001E33034" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001E33034", "uniref90_id": "UniRef90_C4XEK7" }, "psc": { "uniref90_id": "UniRef90_C4XEK7", "product": "Transposase", "uniref50_id": "UniRef50_C4XEK7" }, "pscc": { "uniref50_id": "UniRef50_C4XEK7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEK7" ], "product": "Transposase" }, "genes": [], "id": "OAIKOHHJFB_629", "locus": "GCA000209735_00604" }, { "type": "cds", "sequence": "contig_1", "start": 333193, "stop": 333549, "strand": "+", "frame": 1, "gene": null, "product": "Transposase, IS861", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AEP7" ], "nt": "TTGAAAAAGTCAACTTTTTATGAGATATTAAAATCACAAAATAAACCTGATAAAGATGAAAATTTAAAAAAGGTTATTTTTGGCTTATTTAACTATAATAAAGGACTATACGGTTATAGACGTATTACTTTTGCTTTAAGAAATAAAGGAATAATAATCAATCATAAAAAAGTTCAAAAATTAATGAAATCATTAAATCTTTTTGGCAAAACATTAAGAAGAAAAAATAAGTATTCTTCATTCAAAGGCGATGCTCACAAAAACATTCCAAACTTACTTTTAGAAAAAGAAAATATAATAAAAGATTTTTTCAGACATATAAGAAATTTTTCTAATGAAAAATATTTAGATATATGA", "aa": "MKKSTFYEILKSQNKPDKDENLKKVIFGLFNYNKGLYGYRRITFALRNKGIIINHKKVQKLMKSLNLFGKTLRRKNKYSSFKGDAHKNIPNLLLEKENIIKDFFRHIRNFSNEKYLDI", "aa_hexdigest": "a43f80955edde4aaaabafb4298700a2b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A449AEN5", "query_cov": 0.8135593220338984, "subject_cov": 0.8727272727272727, "identity": 0.51, "score": 83.2, "evalue": 1.98e-18, "valid": false, "product": "Transposase, IS861", "uniref50_id": "UniRef50_A0A449AEP7" }, "pscc": { "uniref50_id": "UniRef50_A0A449AEP7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AEP7" ], "product": "Transposase, IS861" }, "genes": [], "id": "OAIKOHHJFB_630", "locus": "GCA000209735_00605" }, { "type": "cds", "sequence": "contig_1", "start": 333781, "stop": 334029, "strand": "+", "frame": 1, "gene": null, "product": "IS3 family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0003B3129C" ], "nt": "TTGAAAGAAAAAAATGCAATTCAAAGCATGTCAAGAAAAGGAAATTGTTTGGACAACAGCCCAACAGAATGTTTATTTAGTGTAATCAAAAGAGAATTTTGGTTTGGTGAAGAAAAGAAATTTAATAGTTTTAAAGAATTTAAAACTGCTTTAGAAGAATATATTTCATATTATAATAATGACAGAATTGTTAATAAATTAAAAGGACTTAGCCCTGTTCAATACAGGAATAAGTCCAAACATAATTAA", "aa": "MKEKNAIQSMSRKGNCLDNSPTECLFSVIKREFWFGEEKKFNSFKEFKTALEEYISYYNNDRIVNKLKGLSPVQYRNKSKHN", "aa_hexdigest": "2886c266660afe5f4866afcaa139d6a3", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0003B3129C", "query_cov": 0.9878048780487805, "subject_cov": 1.0, "identity": 0.877, "score": 143.0, "evalue": 3.96e-43, "valid": false, "product": "IS3 family transposase", "uniref50_id": "UniRef50_UPI0003B3129C" }, "pscc": { "uniref50_id": "UniRef50_UPI0003B3129C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0003B3129C" ], "product": "IS3 family transposase" }, "genes": [], "id": "OAIKOHHJFB_631", "locus": "GCA000209735_00606" }, { "type": "tRNA", "sequence": "contig_1", "start": 334166, "stop": 334239, "strand": "+", "gene": "trnG", "product": "tRNA-Gly(tcc)", "amino_acid": "Gly", "anti_codon": "tcc", "score": 79.5, "nt": "GCGGATGTAGTTCAATGGTAGAACTTCAGCCTTCCAAGCTGACTATGCGGGTTCGATTCCCGTCATCCGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 334198, 334200 ], "id": "OAIKOHHJFB_4", "locus": "GCA000209735_00607" }, { "type": "cds", "sequence": "contig_1", "start": 334339, "stop": 335088, "strand": "-", "frame": 2, "gene": null, "product": "PTS system, glucose/glucosamine/beta-glucoside-specific, IICBA component", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B3Q9", "UniRef:UniRef90_C4XEK9" ], "nt": "ATGGGTGGAATTGAAAATATTACAGGTTTTAATAACTGTGCTGTTCGTCTTAGATATGATGTTAAAGATACTTCATTAGTAAATGAAGAAGCTCTTAAAAAAGTTGGGGCAAGTGAAGTTATTTTTATTGGAAAAAGACATGTGCAAGCTAAATTTGGTGAAATTAGTGAAAAATTAAATTTAGATATTCAAGAAGCTGCACCATTCTTAGAAGAAGAAGCACTTAAAAATAAAAAAATTGAAGTTAAAAGTGATGTCAACTTATTTGAAGAAAAACCGGTTGAAGTTAAAAAAACTGTTGATGAAAATATAGTTTATACACCTTGCAAAGGTACAAGAAAATCAATTGAAGATTTAAGTGATCAAACCTTTAGTTTGCTAGGTTGTGGTTATGCAATTGAAATTGATAAAAAATTAAACAAATTAGAAGTATATGCACCAATTAATGGAAAATTAATTATGTCATATCCAACTAAACATGCATATGGTATAGCAAATGATAATGGTCTTGAAGTTTTAGTACATATTGGTATTGATACAGTTAAATTAAATGGTTTAGGCTTTGAAACAAATCTTAAACAAAATGATAAAGTTGAAAGAGGACAACTCTTAGCAACTGTTGATCTTAAGAAATTAAGAAATAGTAAAATTTGTAGTGATGTTATTGTTTTAATTACAGATAATGTTAATAAAAACAAACAAACAAAATTGCTTTGTCCTTTAGAAATCAAAGAAGTAAATGTTCCTTGA", "aa": "MGGIENITGFNNCAVRLRYDVKDTSLVNEEALKKVGASEVIFIGKRHVQAKFGEISEKLNLDIQEAAPFLEEEALKNKKIEVKSDVNLFEEKPVEVKKTVDENIVYTPCKGTRKSIEDLSDQTFSLLGCGYAIEIDKKLNKLEVYAPINGKLIMSYPTKHAYGIANDNGLEVLVHIGIDTVKLNGLGFETNLKQNDKVERGQLLATVDLKKLRNSKICSDVIVLITDNVNKNKQTKLLCPLEIKEVNVP", "aa_hexdigest": "c2732983f364223a965ba0d7fe98c794", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XEK9", "query_cov": 1.0, "subject_cov": 0.8469387755102041, "identity": 1.0, "score": 483.0, "evalue": 1.84e-171, "valid": true, "uniref50_id": "UniRef50_A0A449B3Q9" }, "pscc": { "uniref50_id": "UniRef50_A0A449B3Q9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B3Q9" ], "product": "PTS system, glucose/glucosamine/beta-glucoside-specific, IICBA component" }, "genes": [], "id": "OAIKOHHJFB_632", "locus": "GCA000209735_00608" }, { "type": "cds", "sequence": "contig_1", "start": 335447, "stop": 335659, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00389.36" ], "nt": "ATGAAAATAGCTTTTTTTGATGCAAAGGAATATGATAAAAAATATTTTGATGCAGTAAATAATGGACGACATGAAATTAAGTATTTCGACGAAAATTTAAACATTAACACAGTAACTAAAGCTAAAGGGTTTGATGCTATTTGTGCTTTTGTTAATACTTATGGTGACAAATACATTCTTGAACTTTTAGCCAAGATGGGTGTCAAGGTTTGA", "aa": "MKIAFFDAKEYDKKYFDAVNNGRHEIKYFDENLNINTVTKAKGFDAICAFVNTYGDKYILELLAKMGVKV", "aa_hexdigest": "24160e7e57cd710d2fea81ee33e9c68b", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00389.36", "name": "2-Hacid_dh", "length": 63, "aa_cov": 0.8857142857142857, "hmm_cov": 0.9523809523809523, "evalue": 2.091459232982839e-07, "score": 26.80187225341797, "start": 3, "stop": 64 } ], "seq_stats": { "molecular_weight": 8092.2386, "isoelectric_point": 7.8017866134643565 }, "id": "OAIKOHHJFB_633", "locus": "GCA000209735_00609" }, { "type": "cds", "sequence": "contig_1", "start": 335672, "stop": 336310, "strand": "+", "frame": 2, "gene": null, "product": "D-lactate dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEL0", "UniRef:UniRef90_C4XEL0" ], "nt": "ATGGGATACAACAAAATTGATTTAACAAAAGCTGCTGAATTAGGAATCTCAGTTTTTAGAGTACCTAATTACTCAGCCGAAAGTGTTGCTGAATTTGCAGCTGGAACTTTATTAGCTTTAAATAGAAATATTGTAAAAGCTGAAAAATTAGTATCAAAATATAACTTCTCACTTAATGGTCTAGATGGTAAAGCAATTCATGGTTCAACTGTGGGTGTTATTGGTGGCGGAAAAATTGGACAATGTTTTATAAGAATTATGAAAGGTATGGGAGCTAGAATCTTAGTATTTGATGCTTATAACCAAAAACACTTTCCTAATTTAGCAACTGAATTAGGTTTTGAATATGCTTCATTTACTAAAGTATTAAAAGAAAGTGACTTTATTTCACTTCATGCGCCTTTATTACCTTCAACAACTCACTTAATTGACAAAGATGCAGTTAAAATAATGAAAAAAGGTGTGATCTTAGTTAATACAGCTAGAGGTGAATTAATTGATATTCCCGGTGTTCTTTATGGACTTAAAAAAGGAATTATTAGAGGACTTGCCAGTGATGTTTTAGAAAGAGAAGAAGGAAGATTCTACCAAGATATTTCAAGTGAAGCAAATGAATACAAAAAGAATGATCCTGAATGA", "aa": "MGYNKIDLTKAAELGISVFRVPNYSAESVAEFAAGTLLALNRNIVKAEKLVSKYNFSLNGLDGKAIHGSTVGVIGGGKIGQCFIRIMKGMGARILVFDAYNQKHFPNLATELGFEYASFTKVLKESDFISLHAPLLPSTTHLIDKDAVKIMKKGVILVNTARGELIDIPGVLYGLKKGIIRGLASDVLEREEGRFYQDISSEANEYKKNDPE", "aa_hexdigest": "861682a6d127cd52b70f2926599b40dd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 335432, "stop": 336484, "observations": { "mutation": [ 335657, 336308 ], "stop": [ 335657, 336308 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEL0", "query_cov": 1.0, "subject_cov": 0.6057142857142858, "identity": 1.0, "score": 411.0, "evalue": 6.34e-143, "gene_start": 1, "gene_end": 212, "reference_start": 81, "reference_end": 292, "reference_sequence": "MLGEKMKIAFFDAKEYDKKYFDAVNNGRHEIKYFDENLNINTVTKAKGFDAICAFVNTYGDKYILELLAKMGVKVWLQRSMGYNKIDLTKAAELGISVFRVPNYSAESVAEFAAGTLLALNRNIVKAEKLVSKYNFSLNGLDGKAIHGSTVGVIGGGKIGQCFIRIMKGMGARILVFDAYNQKHFPNLATELGFEYASFTKVLKESDFISLHAPLLPSTTHLIDKDAVKIMKKGVILVNTARGELIDIPGVLYGLKKGIIRGLASDVLEREEGRFYQDISSEANEYKKNDPEWEELISNENVIITSHQAFLTDVALTQIAKITLENADMAQNKDFSKALVIMENGKVKNG" }, "paralog": false, "identity": 0.9914285714285714, "score": 677.0, "evalue": 1.73e-250, "description": "Internal stop codon at 335657, 336308. Nonsense mutation around 335657, 336308.", "psc": { "uniref90_id": "UniRef90_C4XEL0", "product": "D-lactate dehydrogenase", "uniref50_id": "UniRef50_C4XEL0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEL0", "UniRef:UniRef50_C4XEL0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEL0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEL0" ], "product": "D-lactate dehydrogenase" } }, "id": "OAIKOHHJFB_634", "locus": "GCA000209735_00610" }, { "type": "cds", "sequence": "contig_1", "start": 336885, "stop": 337121, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAATTAGCTATGCAAATAGTTGGCGGAATTTTAGCTGGTTTAATTATTTATGGTATTGGTCATAATGCTTCTGGAATAAAATTTGAAGCTAATTATCTAGCTAATGCAACTGATACAGCACATAAAGCTAACCACTACATTTCAGCACACAATGTAAGCGCAAAATCATTTATGCCTAACTTTGATAGTGCTTTTGCAAAAGAAGCGGTAATTACTAAAAAAACAATTTGA", "aa": "MAKLAMQIVGGILAGLIIYGIGHNASGIKFEANYLANATDTAHKANHYISAHNVSAKSFMPNFDSAFAKEAVITKKTI", "aa_hexdigest": "5de9688470557a7fcbe4adac6461d93b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8279.486499999999, "isoelectric_point": 9.399442100524901 }, "id": "OAIKOHHJFB_635", "locus": "GCA000209735_00611" }, { "type": "cds", "sequence": "contig_1", "start": 337152, "stop": 337253, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTAGGTGCAATTCTTTTATTCCCTATCTTTAGTCCAAGATTAGACAACAAATATCGTGACACATTTATTTGCTTCATTATTAGTTTTGATGTTTGA", "aa": "MVLGAILLFPIFSPRLDNKYRDTFICFIISFDV", "aa_hexdigest": "2db4f4ce743334ed205d39dad0d1a35c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3865.603400000001, "isoelectric_point": 5.791972923278809 }, "id": "OAIKOHHJFB_636", "locus": "GCA000209735_00612" }, { "type": "cds", "sequence": "contig_1", "start": 337254, "stop": 337382, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTATTCTGCTAGGTTCAGCTGCCATTAACCCTGTTCGTGGTTTAGCTCAACAAGTACCTACCTTATTCTTTGATATGAAACACTTAAGTGCTATGGCTCAATATGACATTCACATGGGTACTTGA", "aa": "MGILLGSAAINPVRGLAQQVPTLFFDMKHLSAMAQYDIHMGT", "aa_hexdigest": "463db2fbb911d5eeb66c7da318f8b90c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4545.332000000001, "isoelectric_point": 6.691620445251465 }, "id": "OAIKOHHJFB_637", "locus": "GCA000209735_00613" }, { "type": "ncRNA-region", "class": [ "riboswitch", "SO:0000035" ], "sequence": "contig_1", "start": 337897, "stop": 338069, "strand": "+", "label": "Lysine", "product": "Lysine riboswitch aptamer", "score": 60.4, "evalue": 4.5e-09, "db_xrefs": [ "RFAM:RF00168", "SO:0000035" ], "nt": "AGACGCTATAGAGGAGCTCTTAGTAATAGTATATTAGTTTAGCAGGCATGTGTTGAGACTATATAAAAAGGATATAAGAGCCGAACTGGTGATTGTGGCGGCAATTACTGGTGGTAACAATGCATAACAGTTTGTTACTCTTTGGACCTCAAACCATTGGTGCTATAGCTTTG", "id": "OAIKOHHJFB_44" }, { "type": "cds", "sequence": "contig_1", "start": 338146, "stop": 338370, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTATGAATCTTCAGTATATTTAGAGTTTACAACACTTCAGTTAGTTTGCCCTTTAATTAAGTCAAGATTAGGTATTACACGGCCTTTTCTTCCACAACTAGCACTGATTGCAACAGGCGCAAAAGCAGCTAATGTAGCTAATGGGGCAAAAGATCATAATAATCTTCTTTTTTTATTCATAATTTCCTTTCGATATATTAATATCTTTTTTAATAATAATTAA", "aa": "MYESSVYLEFTTLQLVCPLIKSRLGITRPFLPQLALIATGAKAANVANGAKDHNNLLFLFIISFRYINIFFNNN", "aa_hexdigest": "5f67508648e3a8f79c47ef80c01fa223", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8351.697699999997, "isoelectric_point": 9.42800159454346 }, "id": "OAIKOHHJFB_638", "locus": "GCA000209735_00614" }, { "type": "cds", "sequence": "contig_1", "start": 338951, "stop": 339232, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTCAAAAGCAACCAAAAACTTTACAAATGCTTTAAGATATCCTAACCAATATCTATATGGTTTATATGGTGTAGATAGCAACAAATTCAAAAACAGAAGTGAATTCTTAACTGAAAAAGATGGAAGGGAATTTATTAACTTTACACAACTTGCTGGTAAAGAAGATATTACAAGTTTTCAAGACTTACTTTACAATGTTGCAACATCAAAAGATTTTGTTGCAGCACCCTCACAATACATTAAAGAAACAATGAATTCACCTAAAATTTCTGTATGA", "aa": "MLSKATKNFTNALRYPNQYLYGLYGVDSNKFKNRSEFLTEKDGREFINFTQLAGKEDITSFQDLLYNVATSKDFVAAPSQYIKETMNSPKISV", "aa_hexdigest": "eae2c119c5f30dca23c210a897b02a19", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10662.89459999999, "isoelectric_point": 8.859325981140138 }, "id": "OAIKOHHJFB_639", "locus": "GCA000209735_00615" }, { "type": "cds", "sequence": "contig_1", "start": 339350, "stop": 339883, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTATGCAGGTAGATACTTCTCAGCTGGATACTACCCAGAAGCTTCAGAATATAGATTCTACAAAAATAGATTCTATTTTGATAAAGATTTTGTTGAATCAGAAAAAAACTTTGGTAGAGTTGTTACAAAATACTACTCAACAACTGAAGATGTCTTTGTTGCTCAAATATTTGATCTATATCAAAAAGGTAAATTACATACATTATCATTTGCTCAATTAAACCAACAACAACAACTTGATGTTACCAAAAATCCTCAAAAATATGGACTTGAATATCGTCAATCATTAAATAAATTAAATTCTCCATTTAAATTATTATTTAACACAATCCCTTATGGTAAAAACTTCAATGCTGACACTAAAAACCGATTTCTTAATGTTCATGCAGCAAAATTGTTCTATGGTTTAACAGATAATCAAGTACATAATGGAACAACCCAAGAAGAAACTAATGAAGACCTTTATGTAAACATGCTTTCAGGTAGATCAGTTGCATTTAGATCATTATTAACAGCTGCTGTTAACTGA", "aa": "MLYAGRYFSAGYYPEASEYRFYKNRFYFDKDFVESEKNFGRVVTKYYSTTEDVFVAQIFDLYQKGKLHTLSFAQLNQQQQLDVTKNPQKYGLEYRQSLNKLNSPFKLLFNTIPYGKNFNADTKNRFLNVHAAKLFYGLTDNQVHNGTTQEETNEDLYVNMLSGRSVAFRSLLTAAVN", "aa_hexdigest": "10f3d075eefbfa456539ad7b720d677d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20701.935900000004, "isoelectric_point": 9.079421043395996 }, "id": "OAIKOHHJFB_640", "locus": "GCA000209735_00616" }, { "type": "cds", "sequence": "contig_1", "start": 340265, "stop": 340372, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAAAGTATTCAAAAGTTTGGAATCATTAATTAAAAGTATTGATCCAAGATTGAAACCTTCATATAAAAAATTTGAAAGTTCAGAAAGTGACAAAGCAAAATGA", "aa": "MEKVFKSLESLIKSIDPRLKPSYKKFESSESDKAK", "aa_hexdigest": "c55eec0a479edb08ab97ae7dc1f732df", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4074.6954000000023, "isoelectric_point": 9.456109809875489 }, "id": "OAIKOHHJFB_641", "locus": "GCA000209735_00617" }, { "type": "cds", "sequence": "contig_1", "start": 340736, "stop": 340942, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCATCTGTTATTTCTTCACAATTCTTCGTTTCTTATGTAAAAAAACATACAAATGCTCAAGTATTAACCTTAACAAAAGAAATTATGAACTATATGGGAGTTGTTGTTAACCCTGCTAAAACAATTGCAAATGAAAACTTCGGTCCTACTTTAATTAATAGTGAATATTATGAAGTACCTTTTGCAGCAGATAACAATTTATGA", "aa": "MSSVISSQFFVSYVKKHTNAQVLTLTKEIMNYMGVVVNPAKTIANENFGPTLINSEYYEVPFAADNNL", "aa_hexdigest": "b4d34c1b5fd13e82d0bcc19f350314ac", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7590.5722, "isoelectric_point": 5.5737112045288075 }, "id": "OAIKOHHJFB_642", "locus": "GCA000209735_00618" }, { "type": "cds", "sequence": "contig_1", "start": 341005, "stop": 341313, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTAAGTATATATTCAAGAGAATAGCATTTGCTATTCTCACATTATTTATTATTATTATATTTTCATATTCGATTATTATCGCTTTTCAACAAAACCCTTATCGTATCGCAGCTTTAGAAGAAGGACTTTCAAAATTGGAAGTTCAACAAAAACTTCAACAAGCAGAAGAATTCGATCAAACGCCTGGTATTGTTAAATTATTTAACTATTTAATAAACATTTTCCAATTTAATTATGGCGAAGTTTATGTTTATAAATCAGAATATGAAACAATTCCAGAAATGTTTTTTGAACCCTTACAATGA", "aa": "MVKYIFKRIAFAILTLFIIIIFSYSIIIAFQQNPYRIAALEEGLSKLEVQQKLQQAEEFDQTPGIVKLFNYLINIFQFNYGEVYVYKSEYETIPEMFFEPLQ", "aa_hexdigest": "3b6c2ef26f2827f064866588a9387984", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12151.050899999995, "isoelectric_point": 4.854925346374511 }, "id": "OAIKOHHJFB_643", "locus": "GCA000209735_00619" }, { "type": "cds", "sequence": "contig_1", "start": 341259, "stop": 341492, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAAGATATTAAGGAAGAATGGAGCTAAAACAAATGAAGGAACAGCCACGAAGATAAAAATGAAAATGTTTATCAACGTATCTCAAATTGTTCCTCTTTTATATCCAGCTAAAACGCCAATTGCCACACCTAAAATGGCACTAATAACGAAAGCTGGTAATGAAATTAAAATAGTTCATTGTAAGGGTTCAAAAAACATTTCTGGAATTGTTTCATATTCTGATTTATAA", "aa": "MLKILRKNGAKTNEGTATKIKMKMFINVSQIVPLLYPAKTPIATPKMALITKAGNEIKIVHCKGSKNISGIVSYSDL", "aa_hexdigest": "bd6715f103415e5ef31bed2278136e27", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8372.052799999998, "isoelectric_point": 10.132060813903806 }, "id": "OAIKOHHJFB_644", "locus": "GCA000209735_00620" }, { "type": "cds", "sequence": "contig_1", "start": 341584, "stop": 341805, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTTTAGGTTCTCTTTCAGTTTATACTCTTTACTCAAGAAATCAAGTTATCACAGTTTTAACGTCAAACTATATTTTGATAGCTAAAACTAAAGGTTTAAGTGGTAGCAAAATATTCTTTAAATATGTATTTAGAAATATTTCAATTCCACTTGCAGCAATAATAATTCCTTCATATATTGGTCTATTAAGTGGAAGTATTATTATTGAAAGATTCTGA", "aa": "MTLGSLSVYTLYSRNQVITVLTSNYILIAKTKGLSGSKIFFKYVFRNISIPLAAIIIPSYIGLLSGSIIIERF", "aa_hexdigest": "7287c065c528190c491d732c78dba455", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8089.574099999996, "isoelectric_point": 10.08996295928955 }, "id": "OAIKOHHJFB_645", "locus": "GCA000209735_00621" }, { "type": "cds", "sequence": "contig_1", "start": 341806, "stop": 341985, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAAAAGGTACCTCAGATCTAATCGTTAACGCCTTCCCTAAAGGTGAGACAAATATTGTTATGTTTAACATTTTATTCTTTACCACATTATCCTTATTTACAGAGGTTTTAGTCGATGTAACATTTACATTGATTGACCCTAGAATTAAGTATTCAAAAAGTGGTGGAATTAACTGA", "aa": "MVKGTSDLIVNAFPKGETNIVMFNILFFTTLSLFTEVLVDVTFTLIDPRIKYSKSGGIN", "aa_hexdigest": "b90260ee76ab9263feebfa26e750f73e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6557.626, "isoelectric_point": 6.000572013854979 }, "id": "OAIKOHHJFB_646", "locus": "GCA000209735_00622" }, { "type": "cds", "sequence": "contig_1", "start": 342085, "stop": 342432, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12911.12" ], "nt": "ATGGATAGTAGGACTTTTAATGATAAGTACAATATTTCAGATGAATTAATTCAAAAGATTAAATTAGCTGAAAAAGAAAAAAAGTCAACTAATATTGTTGGTAAACCAAAAATATTGTTTTATGAAATTTGCAAAAGATTTTTCAAAAATCCAGTTGTGCTTATTTCATTTACAGCGTTTATTGCTCTTTTATTAACTGGTATTATAGTTACTCTTACTTCTCCTTATTCTCCAACTGGTTCAATATTAAAAGGTGGTGATGAATTAACTCAAAATTTACCTCCAGTCTTTAGTCCAGTAATAACTAATGCCAGCAATTACGAAACTTTTCAAAGAATGAAAGAATGA", "aa": "MDSRTFNDKYNISDELIQKIKLAEKEKKSTNIVGKPKILFYEICKRFFKNPVVLISFTAFIALLLTGIIVTLTSPYSPTGSILKGGDELTQNLPPVFSPVITNASNYETFQRMKE", "aa_hexdigest": "83bdb90f2be798f2de7b7e896720e0d8", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12911.12", "name": "OppC_N", "length": 59, "aa_cov": 0.4782608695652174, "hmm_cov": 0.847457627118644, "evalue": 1.2275237344674501e-08, "score": 30.81315803527832, "start": 41, "stop": 95 } ], "seq_stats": { "molecular_weight": 13009.031499999983, "isoelectric_point": 9.239431571960449 }, "id": "OAIKOHHJFB_647", "locus": "GCA000209735_00623" }, { "type": "cds", "sequence": "contig_1", "start": 342531, "stop": 342845, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAGCAATAATCAAATAACTGAAGGTGGTGCTTGGAAAATTTCAATGACCCTCATCATTACAGTGTCAAGTCATTTTCCAGCATGGAAACCAAGAATAGCACCAACAGCAACACCAATTACAACCTCAATAGTTGCAACAATTAAAGCAACTTTAATTGAGCTAATTGCACCAAATCAAGCGCCTGTTCAAATATCTGCTTCTTTAACTGTTGTACCAAAAAGAGTATTTAAATGTGGCATTGAAGCTTTGATGTTTTCTATATCTTCTGGTTTTAAAGGATTAATTTTTAAAGCACGTGATCAAATATAA", "aa": "MNSNNQITEGGAWKISMTLIITVSSHFPAWKPRIAPTATPITTSIVATIKATLIELIAPNQAPVQISASLTVVPKRVFKCGIEALMFSISSGFKGLIFKARDQI", "aa_hexdigest": "08d882d11204a7f87a87bd4c5ef4d39b", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11197.12149999999, "isoelectric_point": 10.132383155822755 }, "id": "OAIKOHHJFB_648", "locus": "GCA000209735_00624" }, { "type": "cds", "sequence": "contig_1", "start": 342928, "stop": 343185, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCATTATTACAGTTAAAGATGAAGAATATATTATTGCTGCCAAAAGTGTTGGTGCAAGCACAAGTCGTCAAATATTCTTCCATGCTTTACCAGCAATTTTAGGTAAAATTGCTATGAACTATGTTCGTAGAATTCCATCAATTATTCTTTCTATAGCTTCTTTAGCATTCTTAGGATTCTATAACAAAGATACAGATATTAACTTAGGTAAATTTATTTACGATAACATTAATGATGCAAAAACTAATCCATGA", "aa": "MFIITVKDEEYIIAAKSVGASTSRQIFFHALPAILGKIAMNYVRRIPSIILSIASLAFLGFYNKDTDINLGKFIYDNINDAKTNP", "aa_hexdigest": "b1dc4ed1cb0aa22119e4a5794d206c2c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9483.959199999985, "isoelectric_point": 9.048863029479982 }, "id": "OAIKOHHJFB_649", "locus": "GCA000209735_00625" }, { "type": "cds", "sequence": "contig_1", "start": 343301, "stop": 343624, "strand": "+", "frame": 2, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FDFF16B" ], "nt": "ATGACTTCAGTAAAAGAAAAAAAGAATATTAAAAAGAGAAATGAAGACATTAAAGCTGAAAAATTAGCTAATGATTTATTACTCAAAGTTCGTAATTTAAGAGTCGACTTTAAAGTTGGAAGAAACAAATATTTAAGAATAGTTAGAGGCATTGATTTAGATATTTTCAAAGGTCAAATTGTTGGCCTTGTTGGTGAAAGTGGTAGTGGAAAGAGTGTTACTTCAAAATCATTAATTAATGTTAATGAAGGTGCCACTACAACAGCCGATTTTATGCAAATCGAAGACATTAATTTAAACAAATTCAAAAAAGAAAGACAATGA", "aa": "MTSVKEKKNIKKRNEDIKAEKLANDLLLKVRNLRVDFKVGRNKYLRIVRGIDLDIFKGQIVGLVGESGSGKSVTSKSLINVNEGATTTADFMQIEDINLNKFKKERQ", "aa_hexdigest": "f9cf7bbf4a1e88555798cfcb2bb8ff90", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI002FDFF16B", "query_cov": 0.8411214953271028, "subject_cov": 0.8108108108108109, "identity": 0.611, "score": 101.0, "evalue": 7.27e-26, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI002FDFF16B" }, "pscc": { "uniref50_id": "UniRef50_UPI002FDFF16B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FDFF16B" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_650", "locus": "GCA000209735_00626" }, { "type": "cds", "sequence": "contig_1", "start": 343775, "stop": 344137, "strand": "+", "frame": 2, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223MAZ9", "UniRef:UniRef90_UPI002FE1EF1F" ], "nt": "TTGGTAGGCCTTTTGGACAAATTTGGTATTAGAGATTCTGAACAAGTTTTTGACCGTTACCCACATACTTTAAGTGGTGGTATGAAACAACGTGTTGTTATTACAATGGTTGTTGCATTAAAACCAAGCTTAATTATTGCTGACGAACCTACAACTGCTTTAGACCCAACTGTTCAAGCCTCTGTTCTTGCTTTATTCGAAAAAATTCGTCAAGAAATGAATATTTCAATTATTCTTATTTCTCACAACATTTCTGTTGTTGCTAAATTTTGTGATTATATTTATGTTATGTATGCAGGAAGAATAGTAGAAAAAGGAACACGTAAAGAAATCTTTACTGACCCTTGTCATCCTTATACTTGA", "aa": "MVGLLDKFGIRDSEQVFDRYPHTLSGGMKQRVVITMVVALKPSLIIADEPTTALDPTVQASVLALFEKIRQEMNISIILISHNISVVAKFCDYIYVMYAGRIVEKGTRKEIFTDPCHPYT", "aa_hexdigest": "abc1a5bcb977d74598da640d6d4e3d40", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0022487FDE", "query_cov": 0.9916666666666667, "subject_cov": 0.8623188405797102, "identity": 0.504, "score": 118.0, "evalue": 7.69e-32, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A838SM00" }, "genes": [], "truncated": "both", "pseudogene": { "start": 343697, "stop": 344422, "observations": { "mutation": [ 343739, 344135, 344324, 344414 ], "stop": [ 343739, 344135, 344324, 344414 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FE1EF1F", "query_cov": 1.0, "subject_cov": 0.4979253112033195, "identity": 0.8079999999999999, "score": 197.0, "evalue": 9.43e-62, "gene_start": 1, "gene_end": 120, "reference_start": 27, "reference_end": 146, "reference_sequence": "MGKQLLDALNNNEDWKDKTYSEKKNYLLDLLKTFGLRNVEKIFMSYPHTLSGGMKQRIVITMVVALKPDVIIADEPTTALDPTVQASVLALFEDIRQKMGISIILISHNISVIAKFCDYIYVMYAGRIVERGTKQEIFTDPAHPYTWALISAIPESKEEKLYNIKGTPPDMANLPIGDPFAVRSDYALEIDFIKEPPLIPITQTHAAATWLLSPEAPKIEKSDDLKKRLELFKKAFNHEQK" }, "paralog": false, "identity": 0.7219917012448133, "score": 355.0, "evalue": 1.67e-126, "description": "Internal stop codon at 343739, 344135, 344324, 344414. Nonsense mutation around 343739, 344135, 344324, 344414.", "psc": { "uniref90_id": "UniRef90_UPI002FE1EF1F", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A223MAZ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FE1EF1F", "UniRef:UniRef50_A0A223MAZ9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A223MAZ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223MAZ9" ], "product": "Oligopeptide transport ATP-binding protein OppD" } }, "id": "OAIKOHHJFB_651", "locus": "GCA000209735_00627" }, { "type": "cds", "sequence": "contig_1", "start": 344207, "stop": 344326, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAACTTGCCTCTTGGCGATCCTTTTGCTCCACGTAATGATTATGCTTTAGAAATTGATTTCTATAAAGAACCTCCTTTAATTGAAGTCAGTGAAACTCATGCAGCTGCAACTTGA", "aa": "MANLPLGDPFAPRNDYALEIDFYKEPPLIEVSETHAAAT", "aa_hexdigest": "67c325ea9b5612a2a08640ce93aaf67e", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4317.782400000001, "isoelectric_point": 4.192523765563965 }, "id": "OAIKOHHJFB_652", "locus": "GCA000209735_00628" }, { "type": "cds", "sequence": "contig_1", "start": 344409, "stop": 344807, "strand": "+", "frame": 3, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CA0BF5C" ], "nt": "ATGAATAAAAATATTGTTTTAGATATTAAAGGTCTTAAAAAATACTTTACTAATAATGGAATTACAAATAAAGCTGCAGATGATATTACATTCAATGTTCATGAAGGTGAAATAGTTGGACTTATTGGTGAAAGTGGTAGTGGTAAAACTACTGTTGGTCGTTCACTTTTAAGACTTTATGATGATTTTAATGGTTTTGTAACACTAGATGGAAAAATTGTTAGTGGTGCAAGGATTAGTAGAAAACAAAGAAAACATTTGAGAAGAAATATCCAAATGATTTTCCAAGACCCTCATGCTGCTTTAAATGGTCAAAAGAACATTTATTCAACTCTTAAAGAACCACTTGAAGTTAATGGAATTATTAAAGAAAGAATTCAAGATATCTTTAAAGACTGA", "aa": "MNKNIVLDIKGLKKYFTNNGITNKAADDITFNVHEGEIVGLIGESGSGKTTVGRSLLRLYDDFNGFVTLDGKIVSGARISRKQRKHLRRNIQMIFQDPHAALNGQKNIYSTLKEPLEVNGIIKERIQDIFKD", "aa_hexdigest": "2a23d78d0acce62e0e3ffc4d1872fe20", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00249F2F44", "query_cov": 0.9242424242424242, "subject_cov": 0.953125, "identity": 0.779, "score": 193.0, "evalue": 1.38e-61, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI000CA0BF5C" }, "pscc": { "uniref50_id": "UniRef50_UPI000CA0BF5C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CA0BF5C" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_653", "locus": "GCA000209735_00629" }, { "type": "cds", "sequence": "contig_1", "start": 344937, "stop": 346760, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEL6", "UniRef:UniRef90_C4XEL6" ], "nt": "ATGAATTATGATCAAAAGTTAGATATTGAAGACAATTTCAACAACTTTTATAACTATCTTGAAGAAAAACAAAATATGGAAAGTGAAATTGTTGACAACATGTATTCAAATACAGATAATCTTATTAATTTTTATTTTGAAAAACAAAAAGATTACCGTAGCGGAAATTTGGACTTTGATGAAACTAAGTTAATTAAAGCTAAATCAAACTATGAAGAAATACTTGAATTAACAAAAATATCAAAACAACTATATGAAGCAAAATTACAACTTGCTGAAAAAAGTAAAGAGTTAAAAGAATTAATTAAAAACTATAAAGAAAGAGTTAATGATAATAAAAACTCATTTAAAAACTTTATAGCAGAATACAAAAGTGAAGCTAGAATTTTTAAAGTTACACGTCTAACTTCTTATGATCTAGATTTTTACCTATTTAACTATAAAAATGAGTTAATCAACAAAAAAATAGTTAAAGAAATTAGTTTGATTAAGCCTAACTTAAAATTTATCGAATACCACAAAGTTTGCGACTTAATTGAAGATTTAAAAATTTATTCTAAAGAATTTTTTGAAAAATATTTAACACCTATTAAATATCAAAGAAACGTTATTAAAATTGTTGAAAAAACTATTAAAGACAACTTCAAATTTATATATGAAAAATATTCATTGCTTAACCAACAAGAAAAAGATTCTCATGAAAAAGAAGTTGCTACATTAAAAGAAGAAATCAAAAAAATAAAAAGCTTATTAAAAGAAAAACAAGAACCTTCAAAAACTGCTGAAGACTTAGAAAAAGCAAAACAAAATCTAGATAAAGTTAATAAATTCCATGAAGAAGAAATTGCTAAATATGTTGAAAAAAACAAATTAGAAATTGATGAAATTCAAAGGAATATTAAAGAACAAAGTGATAAATATAATTCACTCAAATCACAACAATCAATCACAGACTCAGAATTCAATAGAGTTAAAGATATATTCTTTAATGAGTTAAATAGAGTTATTGATGAAACCAAAAAAGTTAATACTCTTGAAAATAAAGCAAAAGTAAAAGAATTGACAAAAATCAAAAGTATTTTTAAAACAAAAATTTATGATAAATTGAACACACTTAAATCATTTAACATTGAAGTCAAATATCTTAACAAAGACATTAATGATATTAAGAAACTTCTTGGTATTAAAAACTCAAAAATGATTAAAGTGTTCGGAAATAAAGTTGGTAAATTCATTCAAAAACATTTTGCTAGATTTTATTACCATCATTCAATTTCAAATCTTTTAACTAAAACAACAATTTATAAAGCTTTAGAAGATGTTGGATTACTTAAACAATTTGCTTATCGTTATCCACACGAATTCTCAGGCGGACAACGTCAAAGAATAGTTATTGCTCGTGCTCTAATTATTCAACCTAAAGTAATAGTAGCTGATGAACCTATTGCCTCATTAGACATCTCTATTCAAGCTCAAGTTGTTAACTTATTAAAAGACTTATGCGATAAGAAAAACATTGGTATGATCTTTATTGCTCATGACTTGTCAATGGTTGAATACATTGCTGATAGAGTACAAATTATGCACCTTGGTAAGATTGTCGAAAGTGGCGACACTGAGGCTGTTTATAATAAGCCTATGCACCCTTATACAATTAATCTTTTCAAAGCTGTACCTAAAATTTCAAATGCAAATGAAAAATTTGAAAATGTAAGTTTTGAATTAGATTACCTTAAAGGTCAAGTTTATCCAAATGTTCCTCAACTTTATAGAGTTGAAAATAATCATTATCTTTATGGAACAGAATCACAAGTTGAAGAATGA", "aa": "MNYDQKLDIEDNFNNFYNYLEEKQNMESEIVDNMYSNTDNLINFYFEKQKDYRSGNLDFDETKLIKAKSNYEEILELTKISKQLYEAKLQLAEKSKELKELIKNYKERVNDNKNSFKNFIAEYKSEARIFKVTRLTSYDLDFYLFNYKNELINKKIVKEISLIKPNLKFIEYHKVCDLIEDLKIYSKEFFEKYLTPIKYQRNVIKIVEKTIKDNFKFIYEKYSLLNQQEKDSHEKEVATLKEEIKKIKSLLKEKQEPSKTAEDLEKAKQNLDKVNKFHEEEIAKYVEKNKLEIDEIQRNIKEQSDKYNSLKSQQSITDSEFNRVKDIFFNELNRVIDETKKVNTLENKAKVKELTKIKSIFKTKIYDKLNTLKSFNIEVKYLNKDINDIKKLLGIKNSKMIKVFGNKVGKFIQKHFARFYYHHSISNLLTKTTIYKALEDVGLLKQFAYRYPHEFSGGQRQRIVIARALIIQPKVIVADEPIASLDISIQAQVVNLLKDLCDKKNIGMIFIAHDLSMVEYIADRVQIMHLGKIVESGDTEAVYNKPMHPYTINLFKAVPKISNANEKFENVSFELDYLKGQVYPNVPQLYRVENNHYLYGTESQVEE", "aa_hexdigest": "9653af907871df6126bd86e7819f188a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 344637, "stop": 347060, "observations": { "mutation": [ 344805, 344916, 346758 ], "stop": [ 344805, 344916, 346758 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEL6", "query_cov": 1.0, "subject_cov": 0.6827896512935883, "identity": 1.0, "score": 994.0, "evalue": 0.0, "gene_start": 1, "gene_end": 607, "reference_start": 183, "reference_end": 789, "reference_sequence": "MERYLKMNKNIVLDIKGLKKYFTNNGITNKAADDITFNVHEGEIVGLIGESGSGKTTVGRSLLRLYDDFNGFVTLDGKIVSGARISRKQRKHLRRNIQMIFQDPHAALNGQKNIYSTLKEPLEVNGIIKERIQDIFKDWFDIKENFFYVFKQRALELKLKNLESINLLAEKFFPYWIEKFKKMNYDQKLDIEDNFNNFYNYLEEKQNMESEIVDNMYSNTDNLINFYFEKQKDYRSGNLDFDETKLIKAKSNYEEILELTKISKQLYEAKLQLAEKSKELKELIKNYKERVNDNKNSFKNFIAEYKSEARIFKVTRLTSYDLDFYLFNYKNELINKKIVKEISLIKPNLKFIEYHKVCDLIEDLKIYSKEFFEKYLTPIKYQRNVIKIVEKTIKDNFKFIYEKYSLLNQQEKDSHEKEVATLKEEIKKIKSLLKEKQEPSKTAEDLEKAKQNLDKVNKFHEEEIAKYVEKNKLEIDEIQRNIKEQSDKYNSLKSQQSITDSEFNRVKDIFFNELNRVIDETKKVNTLENKAKVKELTKIKSIFKTKIYDKLNTLKSFNIEVKYLNKDINDIKKLLGIKNSKMIKVFGNKVGKFIQKHFARFYYHHSISNLLTKTTIYKALEDVGLLKQFAYRYPHEFSGGQRQRIVIARALIIQPKVIVADEPIASLDISIQAQVVNLLKDLCDKKNIGMIFIAHDLSMVEYIADRVQIMHLGKIVESGDTEAVYNKPMHPYTINLFKAVPKISNANEKFENVSFELDYLKGQVYPNVPQLYRVENNHYLYGTESQVEEWVKPFNINSFSLATNKSIDEEYNSKIDHKPYDGHSEIPNNIEYTDEIKIDEPTCEIKISSNFDFSKPIVEVVETKVSTTKTTKKRSTSRKASSAKKTTKK" }, "paralog": false, "identity": 0.9962825278810409, "score": 1400.0, "evalue": 0.0, "description": "Internal stop codon at 344805, 344916, 346758. Nonsense mutation around 344805, 344916, 346758.", "psc": { "uniref90_id": "UniRef90_C4XEL6", "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_C4XEL6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEL6", "UniRef:UniRef50_C4XEL6" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEL6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEL6" ], "product": "ABC transporter domain-containing protein" } }, "id": "OAIKOHHJFB_654", "locus": "GCA000209735_00630" }, { "type": "cds", "sequence": "contig_1", "start": 347188, "stop": 347559, "strand": "+", "frame": 1, "gene": null, "product": "DUF3899 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEL7", "UniRef:UniRef90_C4XEL7" ], "nt": "TTGTATCGTACATATAAAACTGAAATTCCTTATAAAACTAATGTTAGTGACACACTATTACTAATAGGCGCAATATTGTTAGCTTATTCAATTGTAATCATTTTAGTCACCTTAGGTTTTGGTACCGCCTTATTCAAAAACTTAAGAAATAATTCTTTAACAAGAACTAAAAATGAATTAGAAGCTGAAAAAAGAAAACCAGCTTCAGAAGAACAAAGAGCCAAAATAAAAGTTCTTGAAAAAGAAATTGAAAGAAAAACTAGAAAAATTGAAGCGAGCGAAAACAAAAAAATTAATCGTTTTATATATTATTTGATGCTAATAATAGGATCGATACTCCTTATTTCCAGCGCCATAGTGGGTTATATGTAA", "aa": "MYRTYKTEIPYKTNVSDTLLLIGAILLAYSIVIILVTLGFGTALFKNLRNNSLTRTKNELEAEKRKPASEEQRAKIKVLEKEIERKTRKIEASENKKINRFIYYLMLIIGSILLISSAIVGYM", "aa_hexdigest": "4f93706ad97d0fb4bb6cc06fbf692075", "start_type": "TTG", "rbs_motif": "4Base/6BMM", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 347077, "stop": 347559, "observations": { "mutation": [ 347122 ], "stop": [ 347122 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEL7", "query_cov": 1.0, "subject_cov": 0.76875, "identity": 0.992, "score": 214.0, "evalue": 1.62e-69, "gene_start": 1, "gene_end": 123, "reference_start": 38, "reference_end": 160, "reference_sequence": "MTMNSTREYFREAFTWKKLLHLFIILLISLIAGVSLYLYRTYKTEIPYKTNVSDTLLLIGAILLAYSIVIILVTLGFGTALFKNLRNNSLTRTKNELEAEKRKPASEEQRAKIKVLEKEIERKTRKIEASENKKINRFIYYLMLIIGSILLISSAIVGYM" }, "paralog": false, "identity": 0.9875, "score": 298.0, "evalue": 6.83e-105, "description": "Internal stop codon at 347122. Nonsense mutation around 347122.", "psc": { "uniref90_id": "UniRef90_C4XEL7", "product": "DUF3899 domain-containing protein", "uniref50_id": "UniRef50_C4XEL7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEL7", "UniRef:UniRef50_C4XEL7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEL7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEL7" ], "product": "DUF3899 domain-containing protein" } }, "id": "OAIKOHHJFB_655", "locus": "GCA000209735_00631" }, { "type": "cds", "sequence": "contig_1", "start": 347651, "stop": 348736, "strand": "+", "frame": 2, "gene": null, "product": "Cytosol aminopeptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449ADP4" ], "nt": "ATGTCAAACTTATATCAAACTAAAAGAGACGATAATATTTTGTTAAAAGCTTACTATGATGCAAACAAAAATAAAGAAATTAAAATTGACCAATTAAACTATAAAACAGGCAAAATAACCGAATTTTTAAATAAAAAAGAGGCTTATATTTTCTTAGATAAAAACTTCAAAAATTATGATCAACTTGTTGATTTAATTGATACAAAAATAGTTACTAAAAATAGAAATTATCAAATAGATTTAGAAAGCTTTGTTAATAAAGATTTTCCTATTGAATCAGTACTAAAAGCATTCTATTCAAGAATCATTTTTTACACCTACAACTACTTCAATAAGAAAAAAGAAGCACCTAAAAAGAATCAATATACATTTTTATTAAAAGATAACTCACACAAAGAATTAGCTAACAAATTTGAACTAATTGCAAACAATAGAAACTTAGTAAGAAACTTACAAGTTATGCCAGAAAACTTCTGTAATTCAGAACAATTAGCTCAATACATAGTTAATGATTTTAAAAATGAAAAAGATTTAAAAGTAACTGTTTTAAACAAAAAAGAAATTGAAAAATTAAACATGGGTTTAATTATTTCAGTAAACAAAGGTTCAACACATGAACCACGTGTAGTTATTGTTGAATATAATGGAGACCCTAATAGTAAAGAAAAAATAGTTTATGTAGGTAAAGGAATTACATTTGATACTGGTGGTATGAATACTAAAGGTTATCACATGGAAGGCATGAAATATGACATGTCAGGTAGTGTTATAGTTGCTTACACAGTTAAAACATTAGCTCAATTAAAAGCTAAGAAAAATGTAGCTGCTATTATGTGTATTACTGATAATAGACAAGATGGTGATGCTTCACTTCCAGAAAACGTTTATGAATCAATGAGCGGAATCACTGTTGAAGTAACTGATACTGATGCTGAAGGTCGTTTAGTATTAGCTGATGGTCTTTACTATGGAGCTAAAAAACTTAATGCTACAACTCTTGTTGATGTTGCTACTTTAACTGGAGCTGTTTTAAGAGCTCTTGGAACAGTTTATTCGGGAATTTATTCAACAGATGAAAAGAACTGA", "aa": "MSNLYQTKRDDNILLKAYYDANKNKEIKIDQLNYKTGKITEFLNKKEAYIFLDKNFKNYDQLVDLIDTKIVTKNRNYQIDLESFVNKDFPIESVLKAFYSRIIFYTYNYFNKKKEAPKKNQYTFLLKDNSHKELANKFELIANNRNLVRNLQVMPENFCNSEQLAQYIVNDFKNEKDLKVTVLNKKEIEKLNMGLIISVNKGSTHEPRVVIVEYNGDPNSKEKIVYVGKGITFDTGGMNTKGYHMEGMKYDMSGSVIVAYTVKTLAQLKAKKNVAAIMCITDNRQDGDASLPENVYESMSGITVEVTDTDAEGRLVLADGLYYGAKKLNATTLVDVATLTGAVLRALGTVYSGIYSTDEKN", "aa_hexdigest": "d4d4bd4bd3e170e8dd229e1ff8ff588f", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI00068F9161", "query_cov": 1.005540166204986, "subject_cov": 0.9784366576819407, "identity": 0.6940000000000001, "score": 481.0, "evalue": 9.9e-168, "valid": false, "product": "M17 family metallopeptidase", "uniref50_id": "UniRef50_A0A449ADP4" }, "pscc": { "uniref50_id": "UniRef50_A0A449ADP4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449ADP4" ], "product": "Cytosol aminopeptidase" }, "genes": [], "id": "OAIKOHHJFB_656", "locus": "GCA000209735_00632" }, { "type": "cds", "sequence": "contig_1", "start": 348785, "stop": 349015, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00883.26" ], "nt": "ATGCCTCTTCATGAAGATTTCCATGAGCCAAATACTGCTTCAATAGTTGCTGATTTAAATAACTATAATAATGATGAAAAATCAGACTGTAATACAGCTGCAATGTTCTTAAAACAATTTACAAATGATGTTCCTTACATTCACTGTGATGTTGCAGGTACTGCAGACCTTAGTGGTAAACCACAAGGCGTTTTAGTAGATACTTTAGTTGAATTTGCTTTATTAAAATAA", "aa": "MPLHEDFHEPNTASIVADLNNYNNDEKSDCNTAAMFLKQFTNDVPYIHCDVAGTADLSGKPQGVLVDTLVEFALLK", "aa_hexdigest": "2d8095404762afb20e369da7dd37587b", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00883.26", "name": "Peptidase_M17", "length": 77, "aa_cov": 0.9078947368421053, "hmm_cov": 0.974025974025974, "evalue": 3.8645883716581904e-14, "score": 48.7059440612793, "start": 1, "stop": 69 } ], "seq_stats": { "molecular_weight": 8368.247, "isoelectric_point": 4.345022773742676 }, "id": "OAIKOHHJFB_657", "locus": "GCA000209735_00633" }, { "type": "cds", "sequence": "contig_1", "start": 349225, "stop": 349716, "strand": "+", "frame": 1, "gene": null, "product": "AAA domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MUD9" ], "nt": "ATGGAAATAAAAAGAGATTTTTACTTGAATAAATTAATAAATAAAAAACATAATAAATTAATAAAAATAGTTACTGGTCCAAGAAGAGTAGGTAAAAGTTATTTACTCTTTAATTTGTTTTATCATCATCTTTTACTAGAAGGTGTTAAAAATGATCAAATAATAAAAATAGCTTTAGATTCTATAGAAAATGAGCATTTAAGAAATGGCAAAGTTTTATTTGATTATGTCTTAAATTTTATTAAAGATGAAAACAAATATTATTATGTTCTATTAGATGAAATACAATATGTTTCAAAATTTATTGATGTTTTGAATGGCTTAATGAAACGTGAAAACATTGATATTTATGTGACTGGTAGTAATTCAAAATTATTATCAAGTGACATTGCAACAGAATTCGCTTCTAGAGGTGATGAAGTTAGAATATATGCTTTAAATTTTCAAGAATTCAAAAGCATTAATTTAAATAAAGATAATGAACAACTTTGA", "aa": "MEIKRDFYLNKLINKKHNKLIKIVTGPRRVGKSYLLFNLFYHHLLLEGVKNDQIIKIALDSIENEHLRNGKVLFDYVLNFIKDENKYYYVLLDEIQYVSKFIDVLNGLMKRENIDIYVTGSNSKLLSSDIATEFASRGDEVRIYALNFQEFKSINLNKDNEQL", "aa_hexdigest": "cd0eed35ea2c69e80d192ebeb0188957", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_Q6MUD9", "query_cov": 1.0552147239263803, "subject_cov": 0.8349514563106796, "identity": 0.512, "score": 173.0, "evalue": 4.4e-52, "valid": false, "product": "AAA domain-containing protein", "uniref50_id": "UniRef50_Q6MUD9", "kegg_orthology_id": "K07133" }, "pscc": { "uniref50_id": "UniRef50_Q6MUD9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MUD9" ], "product": "AAA domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_658", "locus": "GCA000209735_00634" }, { "type": "cds", "sequence": "contig_1", "start": 349741, "stop": 350475, "strand": "+", "frame": 1, "gene": null, "product": "ATPase", "db_xrefs": [ "KEGG:K07133", "SO:0001217", "UniRef:UniRef50_C4XEL9", "UniRef:UniRef90_C4XEL9" ], "nt": "ATGCCAAATACTGTTTTAAATGAAAACAGTGAAGAAAAAGAAAACTTTTTGCAAAACCTTTTTCAAAATACATACATGAATGATGTGATTTTAAGATATAAATTAAGAAACAAAGAATTTCTCAATGAAATAATAACTTTTCTTGCAAATAATATTGGATCATATATTAACACCAATAAAATATTTAATTATTTTAAATCATTAAAAAAGACAAATATAAGTTACAACACAATTAGTAAATATTTAGTCTATTTCAATGAAGCTTTTATTATTGAAAAAGCAAATAAACAAAGTGTTAAAAGTTATGAAGTGTTTAAAAGTTTAAACAAATACTATTTTGTTGATCTTGGTATTAGAAACTTTTTAACTAATATCTCTTCAAAAGATGAAGGACATATAATGGAAAATATTATCTTTCTAGAATTAAAAAGAAGAGGTTTCAAAGTATCTGTTGGTTCTCTAGAAAAAAGAGAATTAAACAGTAAAAATGAGTATATTAGAAAAGAATTTGAAATAGATTTTATTGCTAAAAAGAAAAATCAAACTTACTATATTCAAAGATGTCTTAATTTAAATGAAAGTAATGAAGAAAGAGAGAAAATACCTCTTAAAATGCTTGAAGATGCTCATCGACGAATCATCATAACTAAGGATGCTTTTACCTATAAAACTGATAATTACGGCATAGAAACAATTAACTTATTCGACTTTTTATTAAATGATAACATTTTTTAA", "aa": "MPNTVLNENSEEKENFLQNLFQNTYMNDVILRYKLRNKEFLNEIITFLANNIGSYINTNKIFNYFKSLKKTNISYNTISKYLVYFNEAFIIEKANKQSVKSYEVFKSLNKYYFVDLGIRNFLTNISSKDEGHIMENIIFLELKRRGFKVSVGSLEKRELNSKNEYIRKEFEIDFIAKKKNQTYYIQRCLNLNESNEEREKIPLKMLEDAHRRIIITKDAFTYKTDNYGIETINLFDFLLNDNIF", "aa_hexdigest": "302a1c02505692db7167977c11a47756", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 349441, "stop": 350475, "observations": { "mutation": [ 349714 ], "stop": [ 349714 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEL9", "query_cov": 1.0, "subject_cov": 0.5795724465558195, "identity": 1.0, "score": 471.0, "evalue": 3.59e-165, "gene_start": 1, "gene_end": 244, "reference_start": 178, "reference_end": 421, "reference_sequence": "MHEVFMEIKRDFYLNKLINKKHNKLIKIVTGPRRVGKSYLLFNLFYHHLLLEGVKNDQIIKIALDSIENEHLRNGKVLFDYVLNFIKDENKYYYVLLDEIQYVSKFIDVLNGLMKRENIDIYVTGSNSKLLSSDIATEFASRGDEVRIYALNFQEFKSINLNKDNEQLWKEYCEFGGMPNTVLNENSEEKENFLQNLFQNTYMNDVILRYKLRNKEFLNEIITFLANNIGSYINTNKIFNYFKSLKKTNISYNTISKYLVYFNEAFIIEKANKQSVKSYEVFKSLNKYYFVDLGIRNFLTNISSKDEGHIMENIIFLELKRRGFKVSVGSLEKRELNSKNEYIRKEFEIDFIAKKKNQTYYIQRCLNLNESNEEREKIPLKMLEDAHRRIIITKDAFTYKTDNYGIETINLFDFLLNDNIF" }, "paralog": false, "identity": 0.997093023255814, "score": 681.0, "evalue": 2.59e-250, "description": "Internal stop codon at 349714. Nonsense mutation around 349714.", "psc": { "uniref90_id": "UniRef90_C4XEL9", "product": "ATPase", "uniref50_id": "UniRef50_C4XEL9", "kegg_orthology_id": "K07133", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEL9", "UniRef:UniRef50_C4XEL9", "KEGG:K07133" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEL9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEL9" ], "product": "ATPase" } }, "id": "OAIKOHHJFB_659", "locus": "GCA000209735_00635" }, { "type": "cds", "sequence": "contig_1", "start": 350914, "stop": 351393, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEM0", "UniRef:UniRef90_C4XEM0" ], "nt": "ATGGCTAAAGCAGTTTATTTTTCAGATTTAAATTTAATTAAAGTAAAATTCCCAAACAATATTAATAAAGATTTAATTACGAGAGTTGCACATAAATTAGACGATATTTGTGAAGAAATGTGCAATCAAAAAAGCGAAATTAGTTTTTATGTTGAAAAAACCTTTCCATTTGGTCAAAAAATGTTAGCTATTGTTGATGACACACTTGCATATGACTTCAACATTATTTTAAAACCTAAAAAAACTATTAATTTAGAAAGTGCGGATTTCAACAAATATATACTTGAAAAGCTACAAAAACATATTTGTCCATTAGGTTTTAGTACTAAAATTGTTGAAAATTCAAAAAGTTTCAATTTAAAATTTACTGATTTCAAAAATTTTTCAATTGATATTGCTTTATACAGCGAAAATAAAAATAATGAAAGATTTGTTCTAGTTAGATCTAAAGATAAAGCTAATAAAATAGTTTACAATTGA", "aa": "MAKAVYFSDLNLIKVKFPNNINKDLITRVAHKLDDICEEMCNQKSEISFYVEKTFPFGQKMLAIVDDTLAYDFNIILKPKKTINLESADFNKYILEKLQKHICPLGFSTKIVENSKSFNLKFTDFKNFSIDIALYSENKNNERFVLVRSKDKANKIVYN", "aa_hexdigest": "0eb8ef20a1ae7f61cb77e947792d89d7", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "both", "pseudogene": { "start": 350713, "stop": 351429, "observations": { "mutation": [ 350847, 350916, 351420 ], "stop": [ 350847, 350916, 351420 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEM0", "query_cov": 1.0, "subject_cov": 0.6680672268907563, "identity": 1.0, "score": 317.0, "evalue": 1.48e-108, "gene_start": 1, "gene_end": 159, "reference_start": 13, "reference_end": 171, "reference_sequence": "MILWKKLEKRKIMAKAVYFSDLNLIKVKFPNNINKDLITRVAHKLDDICEEMCNQKSEISFYVEKTFPFGQKMLAIVDDTLAYDFNIILKPKKTINLESADFNKYILEKLQKHICPLGFSTKIVENSKSFNLKFTDFKNFSIDIALYSENKNNERFVLVRSKDKANKIVYNWTKVYDYEYTGDKVAEIKKYDRIWKETKELFLKKSEKNPDIDNFSHKTAFNLAVDAVYSVFKKYNNF" }, "paralog": false, "identity": 0.9831932773109243, "score": 465.0, "evalue": 4.1e-169, "description": "Internal stop codon at 350847, 350916, 351420. Nonsense mutation around 350847, 350916, 351420.", "psc": { "uniref90_id": "UniRef90_C4XEM0", "uniref50_id": "UniRef50_C4XEM0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEM0", "UniRef:UniRef50_C4XEM0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEM0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEM0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 18536.305899999996, "isoelectric_point": 8.994838523864747 }, "id": "OAIKOHHJFB_660", "locus": "GCA000209735_00636" }, { "type": "cds", "sequence": "contig_1", "start": 352104, "stop": 352253, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAAGGTAATTTATTAACTATTTTAATACCTAAAATTCCCTTAAAAATTCAAGAAAAAATAGTTGAAATCTTGGAGAGATTTCGTATACTAGAAGCGGAGCTGAAAGCGGAGTTGGAAGTGAGGGGGAAGCAATTTGATTTTTGA", "aa": "MTKGNLLTILIPKIPLKIQEKIVEILERFRILEAELKAELEVRGKQFDF", "aa_hexdigest": "0e817188939c189060c96e629294ee33", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5765.892599999998, "isoelectric_point": 8.100919914245605 }, "id": "OAIKOHHJFB_661", "locus": "GCA000209735_00637" }, { "type": "cds", "sequence": "contig_1", "start": 352263, "stop": 352895, "strand": "+", "frame": 3, "gene": null, "product": "Restriction endonuclease subunit S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A5B8JXU3" ], "nt": "TTGTTAAATTTTACCAACTTTGATAAAAATAATTCAAAAGAATTACAATCTATAGGTTGTTTTATTAGTGGTTTAAGATCAAAAAATAAAGATAGTTTTGTCAATGGAAACCAAAGATACGTTTCTTATTTGGATGTTTTCAATAACAAAGAAATTAATTATTTACCTAACAATTTTGTAAAAATATTTGATGATGAAAATCAAAACGATCTTAATTATGGCGATGTTATTTTTTGCGGTTCATCAGAAAATTTTGATGAAACAGGTTATGCATCTGTTTACACTATAAAAAATGATGAAAAAGTTTATTTAAATAGTTTTTCATTTATTTTTAGATTTAAAGATAACAATTTATTTTTGCCTAAATTTTCTAAATATTTTTTTAATTGTAAAGACTTTAGAGACTTACTTCTCAAATGTATAAATGGTGTAACTAGATTTAATTTATCAAAAGAAAAGATGTCAAAAATAAAAATACCTATTCCTCCTATTGAAACCCAAAACAAAATAGTTTCAATACTTGATAAACTATCAGAATATTCTCAAGAAATTAATTCAGGTTTACCTGCTGAAATAGAATTAAGAAGTAAACAATTTAAGTATTACAGAGATCAATTATTAGATTTTAAATAA", "aa": "MLNFTNFDKNNSKELQSIGCFISGLRSKNKDSFVNGNQRYVSYLDVFNNKEINYLPNNFVKIFDDENQNDLNYGDVIFCGSSENFDETGYASVYTIKNDEKVYLNSFSFIFRFKDNNLFLPKFSKYFFNCKDFRDLLLKCINGVTRFNLSKEKMSKIKIPIPPIETQNKIVSILDKLSEYSQEINSGLPAEIELRSKQFKYYRDQLLDFK", "aa_hexdigest": "c8a0ba698c3d30eab1b49210ab2b2522", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A5B8JXU3", "query_cov": 0.9428571428571428, "subject_cov": 0.8284518828451883, "identity": 0.545, "score": 207.0, "evalue": 2.32e-64, "valid": false, "product": "Restriction endonuclease subunit S", "uniref50_id": "UniRef50_A0A5B8JXU3" }, "pscc": { "uniref50_id": "UniRef50_A0A5B8JXU3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A5B8JXU3" ], "product": "Restriction endonuclease subunit S" }, "genes": [], "id": "OAIKOHHJFB_662", "locus": "GCA000209735_00638" }, { "type": "cds", "sequence": "contig_1", "start": 353138, "stop": 354112, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEM2", "UniRef:UniRef90_C4XEM2", "PFAM:PF04200.17", "PFAM:PF13306.12" ], "nt": "ATGAAAAAAATTAAATTATTAACAATTTTACCTACAGCTGCAATAGCCTTGCCTTTTTCTGTAATGAGCTGTGATGGCGGTGGACAAGAAAATAATTCAAGTACAACTACAAAACTTGATCTTTCGGCTGAATTAAATAGCGTTGTAGTTGAATATGACGGTAATAAATCAATTTTAAAACCATCTGAAGTTAAAGTTGAAAAAATTAGAATTACAGGATTTGGATCAGAATATGAAGTTGAGCCAAATTCAATTAATTTAGTTCCTCATGATGATAAAGGTACTTTAGAAGTAACATTCAAATTAAGACAAAAAAATCTAGTTTCTAATGTACAAAGTAAAACAGTAGAAATTAAAAACTTTTTAAATTCTTCTGGCAACGTTGATAAATATAAAGGATATGAACATCTTGTTATTGAAAATGATATTGTTAAAGGAGCAAAAAGTATTCCAGCTGGTGGAATTTTAGAAATTCCAGATGGTGTTACAAGTATTGCTGCAGCTGCTTTCAGTTTCAAAGCCGGAAACTTAAACACAGTTGTAGGATCTAGTGTTACTAAAAATCAAATTACCAGAGTTATTATTCCTGATAGTGTAACAACAATTGGAGCAGCAGCTTTTGCAGAAAATGTAAATTTAGAATCGGTTGTTATGTCTAAAAAAGTTAATAAAATCTCTGAAGCATTATTTGAAGGTTGCGTCAGATTGAAAACTATTAATTTGCCAAATGAAATAGACACAATTGGGAACTATGCTTTCTTAAGATGTAAAGCTTTAACCAAAATTGACTTACCTAAAAATTTAACCTCACTTGGATCAAGCATTGATTTAACAGCTGCTAAAACTTTCCAAGGAACAAGTTTAACAGAAATTACAATTCCAGCTAATGTTAAAATGATTCCTACTTCAACATTTGAAGGTTGTGAAAGCTTATCTAAAGTAACTTTAAATTCAAAATTAGAAAAAGTTTCTTGA", "aa": "MKKIKLLTILPTAAIALPFSVMSCDGGGQENNSSTTTKLDLSAELNSVVVEYDGNKSILKPSEVKVEKIRITGFGSEYEVEPNSINLVPHDDKGTLEVTFKLRQKNLVSNVQSKTVEIKNFLNSSGNVDKYKGYEHLVIENDIVKGAKSIPAGGILEIPDGVTSIAAAAFSFKAGNLNTVVGSSVTKNQITRVIIPDSVTTIGAAAFAENVNLESVVMSKKVNKISEALFEGCVRLKTINLPNEIDTIGNYAFLRCKALTKIDLPKNLTSLGSSIDLTAAKTFQGTSLTEITIPANVKMIPTSTFEGCESLSKVTLNSKLEKVS", "aa_hexdigest": "574849dca9cbecf60bc69a9b7c191e4b", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 353138, "stop": 354379, "observations": { "mutation": [ 354110 ], "stop": [ 354110 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEM2", "query_cov": 1.0, "subject_cov": 0.542713567839196, "identity": 1.0, "score": 464.0, "evalue": 3.29e-158, "gene_start": 1, "gene_end": 324, "reference_start": 1, "reference_end": 324, "reference_sequence": "MKKIKLLTILPTAAIALPFSVMSCDGGGQENNSSTTTKLDLSAELNSVVVEYDGNKSILKPSEVKVEKIRITGFGSEYEVEPNSINLVPHDDKGTLEVTFKLRQKNLVSNVQSKTVEIKNFLNSSGNVDKYKGYEHLVIENDIVKGAKSIPAGGILEIPDGVTSIAAAAFSFKAGNLNTVVGSSVTKNQITRVIIPDSVTTIGAAAFAENVNLESVVMSKKVNKISEALFEGCVRLKTINLPNEIDTIGNYAFLRCKALTKIDLPKNLTSLGSSIDLTAAKTFQGTSLTEITIPANVKMIPTSTFEGCESLSKVTLNSKLEKVSWAAFKGCTSLTEINLPENVKFIGKHAFLNCSNLAKVEMKNKVTEIKEGAFENVTALKNITLSTALTTLSKEAFKNSGITTIALPANLKAIEEGTFENCTALTKVTMGNNITAINSKAFYNCGVLNSINLSTSLVKIQNNAFENTTNLSVVTFPTTITLIDNGAFKNSGLVTLTVPGTLSNVEPESFMNCKKLTTVTFGTGWDNKDIKSKAFYGCDVLTTVTFASKVKNILKEAFFACPLLKEVSIHKGTDWKTGLMNKNKAFDETITTVKVIN" }, "paralog": false, "identity": 0.9975786924939467, "score": 493.0, "evalue": 3.98e-172, "description": "Internal stop codon at 354110. Nonsense mutation around 354110.", "psc": { "uniref90_id": "UniRef90_C4XEM2", "uniref50_id": "UniRef50_C4XEM2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEM2", "UniRef:UniRef50_C4XEM2" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEM2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEM2" ] } }, "hypothetical": true, "pfams": [ { "id": "PF04200.17", "name": "Lipoprotein_17", "length": 81, "aa_cov": 0.22530864197530864, "hmm_cov": 0.9259259259259259, "evalue": 1.763773634838339e-06, "score": 24.336694717407227, "start": 50, "stop": 122 }, { "id": "PF13306.12", "name": "LRR_5", "length": 86, "aa_cov": 0.2654320987654321, "hmm_cov": 0.9883720930232558, "evalue": 3.8388083009310604e-35, "score": 116.65596008300781, "start": 189, "stop": 274 } ], "seq_stats": { "molecular_weight": 34716.519000000044, "isoelectric_point": 8.186598396301271 }, "id": "OAIKOHHJFB_663", "locus": "GCA000209735_00639" }, { "type": "cds", "sequence": "contig_1", "start": 354221, "stop": 354712, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13306.12", "PFAM:PF13855.12" ], "nt": "ATGAAAAACAAAGTTACTGAAATTAAAGAAGGCGCTTTTGAAAATGTTACAGCTTTAAAAAATATTACATTAAGTACAGCTTTAACTACTTTATCAAAAGAAGCATTCAAAAACAGCGGTATTACTACAATTGCCTTACCAGCTAACTTAAAAGCTATTGAAGAAGGAACTTTTGAAAATTGTACTGCTTTAACAAAAGTTACAATGGGCAACAACATAACTGCAATTAATTCTAAGGCTTTCTACAACTGTGGTGTTTTAAATTCAATTAATCTTTCAACTTCATTAGTAAAAATTCAAAACAATGCATTTGAAAATACAACAAATTTAAGCGTAGTTACTTTCCCTACAAAAATTACCTTAATTGATAATGGTGCATTTAAAAATAGTGGTTTAGTAACTCTTACAGTACCCGGAACTTTATCAAATGTTGAACCAGAAAGTTTTATGAATTGTAAAAAACTTACAACTGTAACTTTTGGAACAGGTTGA", "aa": "MKNKVTEIKEGAFENVTALKNITLSTALTTLSKEAFKNSGITTIALPANLKAIEEGTFENCTALTKVTMGNNITAINSKAFYNCGVLNSINLSTSLVKIQNNAFENTTNLSVVTFPTKITLIDNGAFKNSGLVTLTVPGTLSNVEPESFMNCKKLTTVTFGTG", "aa_hexdigest": "01a0997db943ea752b9d7b5a90f958d4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13306.12", "name": "LRR_5", "length": 105, "aa_cov": 0.6441717791411042, "hmm_cov": 0.9904761904761905, "evalue": 7.190878823985005e-48, "score": 157.7946319580078, "start": 2, "stop": 106 }, { "id": "PF13855.12", "name": "LRR_8", "length": 46, "aa_cov": 0.27607361963190186, "hmm_cov": 1.0, "evalue": 3.1197820024748063e-16, "score": 55.0218391418457, "start": 49, "stop": 93 } ], "seq_stats": { "molecular_weight": 17363.746699999985, "isoelectric_point": 8.79028034210205 }, "id": "OAIKOHHJFB_664", "locus": "GCA000209735_00640" }, { "type": "cds", "sequence": "contig_1", "start": 355069, "stop": 356319, "strand": "+", "frame": 1, "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "db_xrefs": [ "EC:5.4.2.12", "GO:0006096", "GO:0046537", "KEGG:K15633", "SO:0001217", "UniRef:UniRef50_Q8EW33", "UniRef:UniRef90_C4XEM3" ], "nt": "ATGAAACGTACAATTTTAGTTGTTATTGATGGTTTAGGCTTACGTGAAGAAAAGCAAGGGAATGGTTTCAAGTTAGCTAAAACTCCAACATTTGATAAATTATTTAAGGAATATCCAAATAGCATTTTACAAGCTAGTGGTGAATATGTTGGCTTACCAAAAGGCCAAATGGGAAATTCTGAAGTAGGTCACTTAAACATTGGAGCTGGAACAGTTGTTTATACTGGTTTAAGCTTAATTAATAAAGCAATGAAAGATGGAGAATTTGAAAAAAACAAAACCTTCAATGAAGTTTTTGCAGATGTTAAAAAGAAAAATTCAACACTTCATTTAATGGGTCTTTTAAGTCCAGGCGGTGTGCATTCACTTGAAGATCACTTATTTAAATTAATTGATATGGCACATGCTTATGGACTTAAAAAAGTATCAGTTCATGCCTTTGGTGATGGTCGTGATGTAGCTCCAAAAAGTATTATCACTTCACTTAGAAAATTACAAACTCAAGTTGACAAATATGGCTATAAAATAGCCTCAATCGGCGGAAGATTCTATGGAATGGACCGTGACAAAATGTTTGATAGAGTTGAAAAACATTACGAAGCATTATTAGGAAATTCAGACCATACATATGAATCAGTTATTGATTATGTAAATAGCCAATATGATGAAGATATTACTGATGAATTCTTTATTCCAGCAATTGACAAAGGTGCAAACTTTGTTAAAGATAATGACTCAATTATTTTCTTTAACTTCCGTCCAGATAGGGCAAGACAATTAACTCATTTATTTATTGGTTCAAAACTTTATGACAATAAACCAAAACATCCAGTTAAAATTTCTAAATTCTGCTCAATGATGAAATATGAAGGAATTAACACTCTAGTAGCATTTAGTGAAATGGAAGTAAAAATGCCTATTGGTCGTGTGTTAGAAAAAGCAGGCAAAAGTCAATTACGTGTTGCAGAAACTCAAAAATATGCACACGTTACCTACTTTATGGATGGTGGAAACGATATTGAATTCAAAAATTCAAAACGAATCATGGTGCCTTCATTAAAGGTTGAATCTTATGCCAATGCTCCTCATATGTCAGCTGAAGGAATTACAGATGAATTAACTAAAAATGTTTTAGATTATGATGTAACAATTATGAACTATGCAAATCCTGACATGGTCGGTCATACAGGAAACTTAAAATCAACTATTGAAGCAGTTAGTTTCTTAGATACTCAAATTAAAAGACTTTGA", "aa": "MKRTILVVIDGLGLREEKQGNGFKLAKTPTFDKLFKEYPNSILQASGEYVGLPKGQMGNSEVGHLNIGAGTVVYTGLSLINKAMKDGEFEKNKTFNEVFADVKKKNSTLHLMGLLSPGGVHSLEDHLFKLIDMAHAYGLKKVSVHAFGDGRDVAPKSIITSLRKLQTQVDKYGYKIASIGGRFYGMDRDKMFDRVEKHYEALLGNSDHTYESVIDYVNSQYDEDITDEFFIPAIDKGANFVKDNDSIIFFNFRPDRARQLTHLFIGSKLYDNKPKHPVKISKFCSMMKYEGINTLVAFSEMEVKMPIGRVLEKAGKSQLRVAETQKYAHVTYFMDGGNDIEFKNSKRIMVPSLKVESYANAPHMSAEGITDELTKNVLDYDVTIMNYANPDMVGHTGNLKSTIEAVSFLDTQIKRL", "aa_hexdigest": "44271c866e7b4d78b026fe7ca9c32834", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_C4XEM3", "query_cov": 1.0, "subject_cov": 0.8205128205128205, "identity": 1.0, "score": 832.0, "evalue": 2.68e-303, "valid": true, "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "ec_ids": [ "5.4.2.12" ], "uniref50_id": "UniRef50_Q8EW33", "kegg_orthology_id": "K15633", "go_ids": [ "GO:0006096", "GO:0046537" ] }, "pscc": { "uniref50_id": "UniRef50_Q8EW33", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8EW33" ], "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase" }, "genes": [ "gpmI" ], "id": "OAIKOHHJFB_665", "locus": "GCA000209735_00641" }, { "type": "cds", "sequence": "contig_1", "start": 356434, "stop": 356568, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCATTTGTACAGATAAAAATATTAAACTTGCAAACGGTAAATTAGCTAATGTTGCACCAACAATTTTGGATTACATTGGTGTTAAACAACCAAAAGAAATGGATGAAAAATCATTATTAATTCGTAAATAA", "aa": "MFICTDKNIKLANGKLANVAPTILDYIGVKQPKEMDEKSLLIRK", "aa_hexdigest": "ea90937aacb199a5afd32d3c427db213", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4961.905999999999, "isoelectric_point": 9.30203037261963 }, "id": "OAIKOHHJFB_666", "locus": "GCA000209735_00642" }, { "type": "cds", "sequence": "contig_1", "start": 356701, "stop": 357132, "strand": "+", "frame": 1, "gene": null, "product": "Sucrose-6F-phosphate phosphohydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8L7G9", "UniRef:UniRef90_C4XEM4" ], "nt": "ATGAAAAATAATAATAAGTTAATTAAAAGAGTTTGCTTTAGCGATGTTGATGGCACAATTTATGGATTTCCTAACAAAGAATTAAAAAATGAAGTAGTTAATTCAGTTAGAAAAGCAAAAGAAGAAAATGATGTCGAATTTGTTATTACAACAGGAAATCCAGCCAATGAAAAAATCAAAAAATTAGCTAAAATAATGGGCTCAAGATATATCTCAACTGCTGGTGGAGCGAGTGTTTTTGATCTTGAAAAAGATGACTATATTCACCTTGAATTAATGGATAAAAAAGAAGCTCAAAAGATCTTTGACTTGTCTAAAAAACATCCTGATTTTGTGCATTTATATTATTTTGGAAAACATCAATTTTATCTTTATAATCCAAGCAAAGAAATGGAAGAATTTCTTAGTGAATTTTGTGAATATTATGACTGA", "aa": "MKNNNKLIKRVCFSDVDGTIYGFPNKELKNEVVNSVRKAKEENDVEFVITTGNPANEKIKKLAKIMGSRYISTAGGASVFDLEKDDYIHLELMDKKEAQKIFDLSKKHPDFVHLYYFGKHQFYLYNPSKEMEEFLSEFCEYYD", "aa_hexdigest": "f630fc66a06dddf3e2a4bf7ded7145ae", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 356701, "stop": 357435, "observations": { "mutation": [ 357130, 357310 ], "stop": [ 357130, 357310 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEM4", "query_cov": 1.0, "subject_cov": 0.4847457627118644, "identity": 1.0, "score": 291.0, "evalue": 1.67e-97, "gene_start": 1, "gene_end": 143, "reference_start": 1, "reference_end": 143, "reference_sequence": "MKNNNKLIKRVCFSDVDGTIYGFPNKELKNEVVNSVRKAKEENDVEFVITTGNPANEKIKKLAKIMGSRYISTAGGASVFDLEKDDYIHLELMDKKEAQKIFDLSKKHPDFVHLYYFGKHQFYLYNPSKEMEEFLSEFCEYYDWNKSGEINQDLHKIELFGTSDEIKKAYEELKKLKLKLKVIYFGTHIEITPKGVDKGYGLKWICENIFNCNFDYVMSIGDSENDIAMFEASGYSYAMDNAKEIAKKAAKYYTSDVEQNGLGEAINDYIFRTHLLLEKQNLEQKLDKQKAKRKY" }, "paralog": false, "identity": 0.9918032786885246, "score": 493.0, "evalue": 1.93e-179, "description": "Internal stop codon at 357130, 357310. Nonsense mutation around 357130, 357310.", "psc": { "uniref90_id": "UniRef90_C4XEM4", "product": "Sucrose-6F-phosphate phosphohydrolase", "uniref50_id": "UniRef50_A0A3P8L7G9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEM4", "UniRef:UniRef50_A0A3P8L7G9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8L7G9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8L7G9" ], "product": "Cof-like hydrolase" } }, "id": "OAIKOHHJFB_667", "locus": "GCA000209735_00643" }, { "type": "cds", "sequence": "contig_1", "start": 357352, "stop": 357588, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGTCAATAGGCGACAGTGAAAACGACATTGCAATGTTCGAAGCTTCGGGCTATTCTTATGCTATGGATAATGCTAAAGAAATTGCTAAAAAAGCAGCTAAATATTACACAAGTGATGTCGAACAAAATGGTTTAGGTGAAGCTATAAATGATTATATTTTTAGAACACACTTATTATTAGAAAAACAAAATTTAGAGCAAAAATTAGATAAGCAAAAAGCAAAAAGAAAATACTAG", "aa": "MSIGDSENDIAMFEASGYSYAMDNAKEIAKKAAKYYTSDVEQNGLGEAINDYIFRTHLLLEKQNLEQKLDKQKAKRKY", "aa_hexdigest": "8c425ea810c83680e259a29abca0aa1d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 49, "aa_cov": 0.6282051282051282, "hmm_cov": 1.0, "evalue": 3.242274254964662e-17, "score": 59.14257049560547, "start": 1, "stop": 49 } ], "seq_stats": { "molecular_weight": 8942.941199999994, "isoelectric_point": 5.769976234436036 }, "id": "OAIKOHHJFB_668", "locus": "GCA000209735_00644" }, { "type": "cds", "sequence": "contig_1", "start": 357882, "stop": 358043, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCTTAATGCTTGTCATGATCATTTTAGGTAGTTACTTCATTGCTCAACCTGATAATGGTTCAGGTAAAAATGTTGAAACATATATTTGTTTAATCTTCTTTGGTGTGTATGGTTTCATTATTACATTGCTATTAATGATTTACTTCTTAATCTTATGA", "aa": "MALMLVMIILGSYFIAQPDNGSGKNVETYICLIFFGVYGFIITLLLMIYFLIL", "aa_hexdigest": "683c983f6bacf6df334e5565c1924d1f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5998.331799999996, "isoelectric_point": 4.3690088272094725 }, "id": "OAIKOHHJFB_669", "locus": "GCA000209735_00645" }, { "type": "cds", "sequence": "contig_1", "start": 358341, "stop": 358439, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAATGTACCTCCAAGAGGCCCACAATTTGGTCCACAACCAAACCCTTATGCACAACAACAAAGTGGAATGTTCAATGTTCAACAAAGACCATGA", "aa": "MFNVPPRGPQFGPQPNPYAQQQSGMFNVQQRP", "aa_hexdigest": "4cad5b797311cc167a306173bb16a06e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3644.063, "isoelectric_point": 10.834379386901855 }, "id": "OAIKOHHJFB_670", "locus": "GCA000209735_00646" }, { "type": "cds", "sequence": "contig_1", "start": 358440, "stop": 358781, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAATGTACCTCCAAGAGGCCCACAATTTGGTCCACAACCAAACCCTTATGCACAACAACAAAGTGGTATGTTTAATGTACCTCCAAGAGGTCCACAATTTGGTCCACAACCAAATCCATATGCACAACAACAAAGTGGTATGTTTAATGTACCTCCAAGAGGTCCACAATTTGGTCCACAACCAAATCCATATGCACAACAACAAAGTGGAATGTTTAATGCACAACCTCAAAATCCACAACCAAACCCATATGGTCAACCAAATCCACAAGATTCAGGTATGTTCCAAGATCCTAATGGTCAAGACTTCGGTGGTTTTGATCCTCAAGGAAACTAA", "aa": "MFNVPPRGPQFGPQPNPYAQQQSGMFNVPPRGPQFGPQPNPYAQQQSGMFNVPPRGPQFGPQPNPYAQQQSGMFNAQPQNPQPNPYGQPNPQDSGMFQDPNGQDFGGFDPQGN", "aa_hexdigest": "c38993dfc5ccf0d0578af36a1f85a9a1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12354.314099999996, "isoelectric_point": 4.5761869430542 }, "id": "OAIKOHHJFB_671", "locus": "GCA000209735_00647" }, { "type": "cds", "sequence": "contig_1", "start": 358945, "stop": 360186, "strand": "+", "frame": 1, "gene": "secA", "product": "preprotein translocase subunit SecA", "db_xrefs": [ "EC:7.4.2.8", "GO:0005524", "GO:0006605", "GO:0006886", "GO:0017038", "KEGG:K03070", "SO:0001217", "UniRef:UniRef50_Q4AAP5", "UniRef:UniRef90_C4XEM6" ], "nt": "ATGGGTTTTAAATTTAAATCAACAGAAATGCTCATTGCAGAAAAAACATTGAAAAAAATTAATCAATTCGAGCCATTAATTTCTCGTTTAAGTGATGATGAACTTAAACAAAAAACACATCAATTTAAGGCTAGACTTGCAGATGGTGAAACTCTCGACAAAATGAGAGCAGAGGTTTTTGCTGTATGTCGTGAAGCTACAAAAAGAGTTTTAGGTAAAAGACCTTTTGATGTGCAAATGATTGGTGGTGTATTGCTTGATTTAAGTTCAATTGCTGAAATGAAAACTGGAGAAGGTAAAACTATTACTTCTATTGCTCCAGTATATTTAAATGCACTTAAAGGCAAGGGCGCAATCGTTTCGACAGTTAATGAATATCTTACTGAACGGGATGCTGAAGAAATAGGCCAAGTATTTAATTTCCTTGGAATGACTGTTGGTGTTAATAAAGCTCAAATGGATCCTTACTTAAAACGTAAAGCTTATGCTTGCGATATTACTTATTCTGTACACTCAGAATTAGGTTTTGATTATTTACGTGATAATATGGCAAAAACAATGGAAGACAAAGTACAACGCGGTTTACACTTTTGTTTAATTGACGAAGCTGACTCTATTCTTATTGATGAAGCTAAAACACCTCTTATTATTTCAGGCGGAGGTGCAACTGTAGATGGTAAGCCATCTAATTCAAATACTTATTATAGTGCTGATCAATTTGTTAGAACTTTAGATGACAAAGACTATGAAATTGATGAAGAAACAAAAGCTATTTCACTAACCTCAAGAGGTATTGAAAAAGCCAACAAATTCTTTAACTTTAAAAATCTTTATGATATTGAAAACTCAGAAATTGTTCATAGAGTTCAAAATGCTTTAAGAGCTCACAAGATTATGAAGAACAATGTTGAGTACATTGTTCGTGAAGGAAAAATTGAATTAGTTGATGCTTTTACTGGACGTGTTATGGAAGGCCGTGCCTATTCAGAAGGTCTTCAACAAGCACTACAAGCTAAAGAAATAGTTGAAATAGAATATGAAACTAGAACCTTTGCAACAATTACTTATCAAAACTTTTTCCGTATGTTTGATAAGCTTTGTGGAATGACTGGTACTGCTAAAACTGAAGAACAAGAATTCATTGACATTTACAACATGCGTGTAAATGTTGTTCCTACAAATAGACCAGTAATTCGTAAAGATTTAAGTGATTCTATTTATGCTTCATATGATGCAAAATGA", "aa": "MGFKFKSTEMLIAEKTLKKINQFEPLISRLSDDELKQKTHQFKARLADGETLDKMRAEVFAVCREATKRVLGKRPFDVQMIGGVLLDLSSIAEMKTGEGKTITSIAPVYLNALKGKGAIVSTVNEYLTERDAEEIGQVFNFLGMTVGVNKAQMDPYLKRKAYACDITYSVHSELGFDYLRDNMAKTMEDKVQRGLHFCLIDEADSILIDEAKTPLIISGGGATVDGKPSNSNTYYSADQFVRTLDDKDYEIDEETKAISLTSRGIEKANKFFNFKNLYDIENSEIVHRVQNALRAHKIMKNNVEYIVREGKIELVDAFTGRVMEGRAYSEGLQQALQAKEIVEIEYETRTFATITYQNFFRMFDKLCGMTGTAKTEEQEFIDIYNMRVNVVPTNRPVIRKDLSDSIYASYDAK", "aa_hexdigest": "8d6ecde115ec653d954f9de4a99d8131", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034361D20", "query_cov": 0.9782082324455206, "subject_cov": 0.9688249400479616, "identity": 0.557, "score": 440.0, "evalue": 4.32e-150, "valid": false, "product": "DEAD/DEAH box helicase", "ec_ids": [ "7.4.2.8" ], "uniref50_id": "UniRef50_A0A939UGC5", "kegg_orthology_id": "K03070" }, "genes": [ "secA" ], "truncated": "3-prime", "pseudogene": { "start": 358942, "stop": 360489, "observations": { "mutation": [ 360184 ], "stop": [ 360184 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEM6", "query_cov": 1.0, "subject_cov": 0.49108204518430437, "identity": 1.0, "score": 804.0, "evalue": 4.96e-287, "gene_start": 1, "gene_end": 413, "reference_start": 2, "reference_end": 414, "reference_sequence": "MMGFKFKSTEMLIAEKTLKKINQFEPLISRLSDDELKQKTHQFKARLADGETLDKMRAEVFAVCREATKRVLGKRPFDVQMIGGVLLDLSSIAEMKTGEGKTITSIAPVYLNALKGKGAIVSTVNEYLTERDAEEIGQVFNFLGMTVGVNKAQMDPYLKRKAYACDITYSVHSELGFDYLRDNMAKTMEDKVQRGLHFCLIDEADSILIDEAKTPLIISGGGATVDGKPSNSNTYYSADQFVRTLDDKDYEIDEETKAISLTSRGIEKANKFFNFKNLYDIENSEIVHRVQNALRAHKIMKNNVEYIVREGKIELVDAFTGRVMEGRAYSEGLQQALQAKEIVEIEYETRTFATITYQNFFRMFDKLCGMTGTAKTEEQEFIDIYNMRVNVVPTNRPVIRKDLSDSIYASYDAKWKAVTEKIKELYEIGQPVLVGTAQIEDSEILHQYLYKANIPHTVLNAKQNASEAEIVSKAGQVKAVTIATNMAGRGTDIKPSPEALELGGLYVIGTDRAESRRIDNQLRGRSGRQGDPGISKFFISLDDQLMRRFSNYEEFKESYALEGDKEITSKSLLHGFEQAQKKIEGFNYDSRKSVLHYDDVIRQQRDLFYAQRDLILINEDISFIVERMVRKTATMLSNYPIFKEKNGLFKHQVFTDYINDVFLGHGTKERLDYEEVKKIYDSEIKDFLIEKLVNFYKKWREQAIRNSNDDTDYINWYEKDLVLEIVDIYWQNHIDTMDKLRSNVNLVQYSQKNPYQVYTDEGSKKFDQMLENIAYDVTKKIFDDRIGIPSIIPYEVQQDDLFKQIKNTIIFDDSLTQEEREQQLLEMYNSIKGQIEQQKEN" }, "paralog": false, "identity": 0.996116504854369, "score": 1002.0, "evalue": 0.0, "description": "Internal stop codon at 360184. Nonsense mutation around 360184.", "psc": { "uniref90_id": "UniRef90_C4XEM6", "gene": "secA", "product": "preprotein translocase subunit SecA", "ec_ids": [ "7.4.2.8" ], "uniref50_id": "UniRef50_Q4AAP5", "kegg_orthology_id": "K03070", "go_ids": [ "GO:0005524", "GO:0006605", "GO:0006886", "GO:0017038" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEM6", "EC:7.4.2.8", "UniRef:UniRef50_Q4AAP5", "KEGG:K03070", "GO:0005524", "GO:0006605", "GO:0006886", "GO:0017038" ] }, "pscc": { "uniref50_id": "UniRef50_Q4AAP5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4AAP5" ], "product": "Protein translocase subunit SecA" } }, "id": "OAIKOHHJFB_672", "locus": "GCA000209735_00648" }, { "type": "cds", "sequence": "contig_1", "start": 360274, "stop": 361038, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF21090.3" ], "nt": "TTGCACCAATATTTATACAAAGCTAATATTCCACATACTGTTTTAAATGCTAAGCAAAATGCTTCAGAAGCTGAAATTGTTTCTAAAGCCGGTCAAGTTAAAGCAGTTACAATTGCAACCAACATGGCAGGTCGTGGTACTGATATTAAACCAAGTCCTGAAGCTTTAGAATTAGGTGGACTTTATGTTATTGGAACAGATAGAGCAGAAAGCCGTAGAATTGACAACCAGCTACGTGGACGTAGTGGACGTCAAGGTGATCCAGGGATTTCAAAATTCTTTATTTCACTTGACGACCAACTTATGAGACGTTTCTCAAACTATGAAGAATTCAAAGAATCTTATGCTTTAGAAGGCGACAAAGAAATAACTTCAAAATCGTTGTTACATGGTTTTGAACAGGCTCAAAAGAAAATTGAAGGGTTCAACTATGATAGTCGTAAAAGTGTTTTACACTATGATGATGTTATCCGTCAACAACGTGATTTATTCTATGCCCAACGCGATTTAATTTTAATCAATGAAGATATCTCATTCATCGTTGAAAGAATGGTTCGTAAAACTGCAACTATGTTAAGTAACTATCCAATTTTTAAAGAAAAGAATGGTTTGTTTAAACACCAAGTATTCACTGATTACATTAATGATGTTTTTCTTGGTCATGGTACAAAAGAACGCTTGGACTATGAAGAAGTTAAAAAAATTTATGACTCAGAAATTAAAGATTTCTTAATTGAAAAATTAGTAAATTTCTATAAAAAATGA", "aa": "MHQYLYKANIPHTVLNAKQNASEAEIVSKAGQVKAVTIATNMAGRGTDIKPSPEALELGGLYVIGTDRAESRRIDNQLRGRSGRQGDPGISKFFISLDDQLMRRFSNYEEFKESYALEGDKEITSKSLLHGFEQAQKKIEGFNYDSRKSVLHYDDVIRQQRDLFYAQRDLILINEDISFIVERMVRKTATMLSNYPIFKEKNGLFKHQVFTDYINDVFLGHGTKERLDYEEVKKIYDSEIKDFLIEKLVNFYKK", "aa_hexdigest": "9aa2252219956fa6b5019b8302c8044b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF21090.3", "name": "P-loop_SecA", "length": 54, "aa_cov": 0.2125984251968504, "hmm_cov": 1.0, "evalue": 4.3901859863143993e-48, "score": 159.6897430419922, "start": 54, "stop": 107 } ], "seq_stats": { "molecular_weight": 29412.01260000001, "isoelectric_point": 7.811456871032715 }, "id": "OAIKOHHJFB_673", "locus": "GCA000209735_00649" }, { "type": "cds", "sequence": "contig_1", "start": 361150, "stop": 361467, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAATTGCGTTCAAATGTTAACTTAGTTCAATATTCTCAAAAGAATCCATACCAAGTTTATACAGATGAAGGTAGCAAGAAATTTGACCAAATGCTTGAAAACATTGCCTATGATGTAACTAAAAAAATCTTTGATGATCGTATAGGTATTCCTTCAATTATTCCTTATGAAGTTCAACAAGATGATTTATTTAAACAAATTAAAAATACAATTATTTTTGATGATTCTTTAACTCAAGAAGAACGTGAACAACAATTACTTGAAATGTACAACAGCATTAAAGGACAAATTGAACAACAAAAAGAAAATTAA", "aa": "MDKLRSNVNLVQYSQKNPYQVYTDEGSKKFDQMLENIAYDVTKKIFDDRIGIPSIIPYEVQQDDLFKQIKNTIIFDDSLTQEEREQQLLEMYNSIKGQIEQQKEN", "aa_hexdigest": "966fb897797673cecf218e001389645b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12499.864199999993, "isoelectric_point": 4.600741386413574 }, "id": "OAIKOHHJFB_674", "locus": "GCA000209735_00650" }, { "type": "cds", "sequence": "contig_1", "start": 361634, "stop": 361759, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATACACCTCAAGACAACAAAAATCATGAAGATAAAAGAGTACAAGTAGAAATAAAAAGATCAAAAGTGAAACAATCATTTTTGCATTTTGGCTCACTTTATCGTGTTAAAAAATTAAGATGA", "aa": "MNTPQDNKNHEDKRVQVEIKRSKVKQSFLHFGSLYRVKKLR", "aa_hexdigest": "3f65eaa5943d982a099af793f7aab63e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4983.7145, "isoelectric_point": 10.446279716491699 }, "id": "OAIKOHHJFB_675", "locus": "GCA000209735_00651" }, { "type": "cds", "sequence": "contig_1", "start": 362438, "stop": 362836, "strand": "+", "frame": 2, "gene": null, "product": "DUF2179 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF5F3CE", "UniRef:UniRef90_UPI000FF5F3CE" ], "nt": "ATGGTTTTAATTAATGGGGTTGTGTTAAATGTTCTATTCCCTAAATATAAAATTGTTAAAGTTGAAATTTATTCATCTAAAATTCCTGAAATTCAAGAAAAAATTAATACTTTAAAAGATGTGAGATTTGCAACAAGCCACTTAAGTGTTAAAGGTGGCTACTCAGGTCAAGAACAAGAAGTTTTAGTTACAATGTGTTTATATTTAGATGCAGCACATCTTCTTGAAATTGTTCGAGAATTTGATGAAAATGCCTTATTTACAGTTACAGACCTTAAAAAGGTAGATGGATATTTCTATGTTTCAGTAGAAAATCACAAGAGTTTATTTAATAAAAAAATTGAAAATAAAGAACAAAAGAAAACTAAAGAAAATGTAGAAGATAACTCGAAAAAATAG", "aa": "MVLINGVVLNVLFPKYKIVKVEIYSSKIPEIQEKINTLKDVRFATSHLSVKGGYSGQEQEVLVTMCLYLDAAHLLEIVREFDENALFTVTDLKKVDGYFYVSVENHKSLFNKKIENKEQKKTKENVEDNSKK", "aa_hexdigest": "d0b89e52f80b83486cc237f32fe05585", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000FF5F3CE", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.992, "score": 246.0, "evalue": 2.92e-82, "valid": true, "product": "DUF2179 domain-containing protein", "uniref50_id": "UniRef50_UPI000FF5F3CE" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF5F3CE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF5F3CE" ], "product": "DUF2179 domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_676", "locus": "GCA000209735_00652" }, { "type": "cds", "sequence": "contig_1", "start": 362898, "stop": 363161, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAATAAAAATAATCAAAATGAATTAACAAACCAAAACGAAATAGAGATTCAACGTTCAAAAATTAAACAGTCGTTTTTGCATTTTGGTACTTTATATAGGGTTAGAAGTTTTAAAAAACAAATAATTTACACCATTATTATTGGAGTTCTTTTTGGTATAGTTCAACATTTACTTATTTCAATAACAGGACTTTATAATATGGGTTTAGACGGACTTTGTCAGTCAATTGCTCGGATAGTTAGACACTTTTTAAGATGA", "aa": "MENKNNQNELTNQNEIEIQRSKIKQSFLHFGTLYRVRSFKKQIIYTIIIGVLFGIVQHLLISITGLYNMGLDGLCQSIARIVRHFLR", "aa_hexdigest": "a108be0960c4f784d15c099e102f04d4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10177.83499999999, "isoelectric_point": 10.006669807434083 }, "id": "OAIKOHHJFB_677", "locus": "GCA000209735_00653" }, { "type": "cds", "sequence": "contig_1", "start": 363609, "stop": 364133, "strand": "+", "frame": 3, "gene": null, "product": "DUF2179 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00217FD9AD", "UniRef:UniRef90_UPI00217FD9AD" ], "nt": "TTGTTATTTTTATTTGCATGTAATTTAATTGGTAGTTATATTCCTTTTGCAGTTGAAGATCCTATTGCAGCTTCTTTTTCAAAACATCCAGGTAATAGATATGAATTAGAAAACTTTTTCAACCCTTCATTTGTCGCCGGATTTATTATGGTTGTAGTTAATGGTATTGTTTTAAATGTTTTATTTCCTAAATTTAAAATGGTTAAAGTTGAAATTATTTCAAATCAAGTTGAAATGATTCAACAAAAATTGAATACTTTAAAAGACCGTCGTTATGCTACAAGTACCTTAACTGTTATTGGTGGTTACAGTCATAAAGAACAAAAAATTCTTTTGACAACTTGCTTATATATTGATGCAGCTAAATTATTAGAAACAGTTCGTGGAATTGACCAAAATGCCTTATTTACAGTTATGGATCTTAAAAAAGCAGATGGTTATATTTATACAACTCACGAACATCAAAATCATTTATTTAAACATAAAAAACGTGACGAAATTAATGATCAACAAAAGCAAAATTAA", "aa": "MLFLFACNLIGSYIPFAVEDPIAASFSKHPGNRYELENFFNPSFVAGFIMVVVNGIVLNVLFPKFKMVKVEIISNQVEMIQQKLNTLKDRRYATSTLTVIGGYSHKEQKILLTTCLYIDAAKLLETVRGIDQNALFTVMDLKKADGYIYTTHEHQNHLFKHKKRDEINDQQKQN", "aa_hexdigest": "a5363b2b2ebc89e51b58f1a7dc4717ad", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00217FD9AD", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.9890000000000001, "score": 337.0, "evalue": 7.01e-117, "valid": true, "product": "DUF2179 domain-containing protein", "uniref50_id": "UniRef50_UPI00217FD9AD" }, "pscc": { "uniref50_id": "UniRef50_UPI00217FD9AD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00217FD9AD" ], "product": "DUF2179 domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_678", "locus": "GCA000209735_00654" }, { "type": "cds", "sequence": "contig_1", "start": 364554, "stop": 364880, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAACAAAAACATGAGAATTGATATTAATCCAAAAAATTATACTTTTACTTTAAAAAAATTAGGTAATAATAAAAAGGAAAAACCAATTAATCTAACTTCGCTTAAATTAACAAAAGAAGCAATTGAAGCTTACAAAAAAACTGGATTGAAAAAACCATCTCAAGTTATAACACATCCTTCAAAACAAATTACAGGTAAAAATGCAGGTTCTCATGGATTTTTATATACAGTTCTTGATAAAAATGGCAAAGTCAACATTATATTTTCAATTAAAGAAAATAATGTAAAATCTAAAGACTTCTTAGCTAAAATTGAAGACTAG", "aa": "MNNKNMRIDINPKNYTFTLKKLGNNKKEKPINLTSLKLTKEAIEAYKKTGLKKPSQVITHPSKQITGKNAGSHGFLYTVLDKNGKVNIIFSIKENNVKSKDFLAKIED", "aa_hexdigest": "81037d5d29170d4cc97526bc406084bd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12219.110099999985, "isoelectric_point": 9.999384880065918 }, "id": "OAIKOHHJFB_679", "locus": "GCA000209735_00655" }, { "type": "cds", "sequence": "contig_1", "start": 365115, "stop": 365432, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATATTCAAGCCACAACAATTAATTTAAATAATTTAATGACATTAAATTTTGATGATGCAACACGTAAATTAACTATTACGGTTTCATACACAACTAAATCAACTCCAAAAGAAAAGAAATTTGAATTAACTTTTGCAGAAAAATCATTTGTAGAATTAAAAGATACATTTTTAGCTGAAATTAATCTTTCATTACCTAACCCTTGTGATGTTACAAAGGCTACAGATAATGACATCATCTATGAAAAAACTAAATTAAATGTACCTACAACAACAAATGACATTTTCACATTAACTGGCGTAACTGTTGATTGA", "aa": "MNIQATTINLNNLMTLNFDDATRKLTITVSYTTKSTPKEKKFELTFAEKSFVELKDTFLAEINLSLPNPCDVTKATDNDIIYEKTKLNVPTTTNDIFTLTGVTVD", "aa_hexdigest": "97c03f957db9da0791b75b28d8881fa9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11798.298099999978, "isoelectric_point": 4.873852729797362 }, "id": "OAIKOHHJFB_680", "locus": "GCA000209735_00656" }, { "type": "cds", "sequence": "contig_1", "start": 365541, "stop": 365891, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAATTAACAAATGTTGACTTAAATGAAAAAGCAAGTAAAACAATTGAAGAATATATTGCAGCTCACACAAGTGATTTATTGGCTCAAGTTACAGCCACAACTCCAAGTGGTAAAACCTTAAGCGAATATCTAAGTGAAGAAAAGATTACAATTAATAAAACAGAATTAATTGTTGCTAATAATAATTTAGGTCAAGCAAAATTGAAATTAACTTTAAGTTTTGAAGATAAAAGTCAAGAAAATGTTGAAGTTGAATATGAAATTAATGATTTCAAAAAACCAGCTCCATATTCAGAAGACAAATTTGAAAACTTAATCGGTACTTTAAAAACTAAAATTAGTTAA", "aa": "MAKLTNVDLNEKASKTIEEYIAAHTSDLLAQVTATTPSGKTLSEYLSEEKITINKTELIVANNNLGQAKLKLTLSFEDKSQENVEVEYEINDFKKPAPYSEDKFENLIGTLKTKIS", "aa_hexdigest": "caff7d0361040972fdad526fa87b35bf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12940.428699999984, "isoelectric_point": 4.931942176818846 }, "id": "OAIKOHHJFB_681", "locus": "GCA000209735_00657" }, { "type": "cds", "sequence": "contig_1", "start": 365970, "stop": 366188, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATTTAAAAGATAAATCAACTAAAACTGCACAAGAATATGTTGAAGAATTTAATGCACTAACTGATCAAGAAAAAGAAGAAAAAATTACATTCACAAATGCAGATTATCAAAATGATTTATCTGTATTTAAAAACATGGAAGGTTTAAATTACATTAAATATCAATTAGAAGTTGTTGACAATTTTGAAGGAAAAGTTAAATTAGTTGCAAAATGA", "aa": "MDLKDKSTKTAQEYVEEFNALTDQEKEEKITFTNADYQNDLSVFKNMEGLNYIKYQLEVVDNFEGKVKLVAK", "aa_hexdigest": "1ff10af9a0e02a06a1b071213972da6d", "start_type": "GTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8431.336199999998, "isoelectric_point": 4.61694049835205 }, "id": "OAIKOHHJFB_682", "locus": "GCA000209735_00658" }, { "type": "cds", "sequence": "contig_1", "start": 366312, "stop": 367229, "strand": "-", "frame": 3, "gene": null, "product": "Lipoprotein-associated type-17 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEN1", "UniRef:UniRef90_C4XEN1" ], "nt": "ATGAAGAAGAAAATTAAATTAAGTCTTATTTTTTCAAGCGTAACAGCAGGTGTTTTACCTTTAATTTCATCTCAATGTCAAAACACAACAAAAGAAGAAATTAAAGCAGATGAAGACTTTAAACTTTTGTCTATTGCAGATATTAAGAATGCTGATTATGACTCTACAAACAAAGTATATAAGATTAGCACAACTACAAAAGAAGCTGATCAAATTAAAAAAGAAGATATTAAATTTGAAAGTACAACAAAGAAATATACATATATAATTGACAACTTAAAAGCAGATAAAGCTAAGGGTGAATTAGAAATTTTCCTTACTCAAAAAGAAGGAAATAAAGAAATTAAAAAATTCACAATTAAGGTTGTAGGATTTTATGTAGATACTGACAAATTTGACGAAAACATCAAATTTATAACTGGTTTGGACTTAGCAGAAAAAGACAAATACACAGTTAAAGAATACAAAAATCAATTTAATGATCTAGCTAAATTACAAGCTAAATTGTCAATAGCAACTTCAAATAGCAAAGATTTAGCTACTTTCTTAAAAGAATATGAAATATCAATTAAAAGCATAGAAATAGAAGAAGTAAGTGCTTCAAAAGGTGAAGCAAATATTAAAGCAACTTTTGAAAATAAAGCAAAAACTAAATCAAAAGAAATTACAATCAAATTAACTGGATTCAAAAAAGAAACTGAATCGTTACAAGCAACATTAGAAGGTATTTTTGATACTATTGATGTTTTAGAAAACAAAAAAGATTTCAATGTTTTCAACTATACAATTACAGATCTAAGATACTTCAAAAATAAAACACAAGTTGATGTAACAAAAGTATTTAATGAAAAAGGTATTGAAATTAAAAATAAAGAAGCTGATACCTCATCAGGTGAAACTGAACCTTATTCAGGATGA", "aa": "MKKKIKLSLIFSSVTAGVLPLISSQCQNTTKEEIKADEDFKLLSIADIKNADYDSTNKVYKISTTTKEADQIKKEDIKFESTTKKYTYIIDNLKADKAKGELEIFLTQKEGNKEIKKFTIKVVGFYVDTDKFDENIKFITGLDLAEKDKYTVKEYKNQFNDLAKLQAKLSIATSNSKDLATFLKEYEISIKSIEIEEVSASKGEANIKATFENKAKTKSKEITIKLTGFKKETESLQATLEGIFDTIDVLENKKDFNVFNYTITDLRYFKNKTQVDVTKVFNEKGIEIKNKEADTSSGETEPYSG", "aa_hexdigest": "ae850df99fd388f214e58ee19d300b84", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 366009, "stop": 367250, "observations": { "mutation": [ 366254, 366287, 366314 ], "stop": [ 366254, 366287, 366314 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEN1", "query_cov": 1.0, "subject_cov": 0.5360281195079086, "identity": 1.0, "score": 558.0, "evalue": 3.09e-196, "gene_start": 1, "gene_end": 305, "reference_start": 8, "reference_end": 312, "reference_sequence": "MLMKEKYMKKKIKLSLIFSSVTAGVLPLISSQCQNTTKEEIKADEDFKLLSIADIKNADYDSTNKVYKISTTTKEADQIKKEDIKFESTTKKYTYIIDNLKADKAKGELEIFLTQKEGNKEIKKFTIKVVGFYVDTDKFDENIKFITGLDLAEKDKYTVKEYKNQFNDLAKLQAKLSIATSNSKDLATFLKEYEISIKSIEIEEVSASKGEANIKATFENKAKTKSKEITIKLTGFKKETESLQATLEGIFDTIDVLENKKDFNVFNYTITDLRYFKNKTQVDVTKVFNEKGIEIKNKEADTSSGETEPYSGWLSINITFGWKDATKTETYVWSQVVAGFKEIKVNSELEAAFNVDLKDKSTKTAQEYVEEFNALTDQEKEEKITFTNADYQNDLSVFKNMEGLNYIKYQLEVVDNFEGKVKLVAKWQQWNQPEYAYNKEITGFVTKETIQQKMAKLTNVDLNEKASKTIEEYIAAHTSDLLAQVTATTPSGKTLSEYLSEEKITINKTELIVANNNLGQAKLKLTLSFEDKSQENVEVEYEINDFKKPAPYSEDKFENLIGTLKTKIS" }, "paralog": false, "identity": 0.9903147699757869, "score": 786.0, "evalue": 1.88e-288, "description": "Internal stop codon at 366254, 366287, 366314. Nonsense mutation around 366254, 366287, 366314.", "psc": { "uniref90_id": "UniRef90_C4XEN1", "product": "Lipoprotein-associated type-17 domain-containing protein", "uniref50_id": "UniRef50_C4XEN1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEN1", "UniRef:UniRef50_C4XEN1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEN1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEN1" ], "product": "Lipoprotein-associated type-17 domain-containing protein" } }, "id": "OAIKOHHJFB_683", "locus": "GCA000209735_00659" }, { "type": "cds", "sequence": "contig_1", "start": 367514, "stop": 367732, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAGAAAAACTAAAATAGGTATTTTAATTGGAACAAATATTATTGTTACTGCTGGACTAATAACAGCTTTAGCAATAGCGCCAACTAGAAATAATAGTAATCATTCAAATTCAGGCACAACAACAAGTGAAACAAGAAAAATAGAAGTCAAAGCTAAAGTGAGTGTTGATTTAAGCAAAAAAATTGATCAGTTATCTAATCCAAGCACTGAGTGA", "aa": "MTRKTKIGILIGTNIIVTAGLITALAIAPTRNNSNHSNSGTTTSETRKIEVKAKVSVDLSKKIDQLSNPSTE", "aa_hexdigest": "f7a31823f30009d5ceca04e301345f8a", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7649.6922, "isoelectric_point": 10.11536350250244 }, "id": "OAIKOHHJFB_684", "locus": "GCA000209735_00660" }, { "type": "cds", "sequence": "contig_1", "start": 369166, "stop": 371175, "strand": "+", "frame": 1, "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "db_xrefs": [ "EC:3.1.25.-", "GO:0003677", "GO:0005515", "GO:0005524", "GO:0006289", "GO:0009380", "GO:0016787", "GO:0016887", "RefSeq:WP_013354505.1", "SO:0001217", "UniParc:UPI0001A8C1E6", "UniRef:UniRef100_C4XEN3", "UniRef:UniRef50_Q8EUN2", "UniRef:UniRef90_C4XEN3" ], "nt": "ATGTCAATTTTTAAATTAATTTCACCTTATAAACCTGCAGGTGATCAACCTGAAGCAATTAAAGAACTTGTAGAAAATATTAAAGACAATATGCAACATCAAGTGCTTAAAGGAGTTACTGGTAGTGGTAAAACTTTTACTATTGCAAATGTTATTAAAGAATTTGATCGCCCTGTTTTAGTTTTAAGTCACAACAAAACTTTAGCTTCTCAACTTTACAGTGAGTTGAAAGGTTTCTTTCCTCATAATCGCGTTGAGTATTATGTTTCATATTTTGACTACTATCGACCCGAAGCTTATATTCCTTCTTCAGATACTTACATTGATAAACAATCTCAAAATAACAAAGATTTAGATGCTATGAGAATGTCAGCTATGAATGCTGTTTTAACTAGAAATGACACTATTATTGTTGCTTCAGTTTCGGCAATTTATGGTGCACTTAATCCTAGTGAATATTATGCTTCAGTTTTTCCTATTGAAGTAGGTATGCAAATTAAAAGGCAAGAATTCTTTAAAAAACTTGTTCAAAGAAACTATAAAAGAAACCAAATTGATTTAGAATTAGGCACTTTTAGCGCCAAAGGTGATGCAGTTTATATTCAACCAGCTGATAGTGATGAATATCAAATTCGAGTCGATTTTTTTGGCGATGAAATTGATTCAATTGCAACTTGCGATCCTATTACTAAAGAAGTAATAGAACGTTTTAGTCATTACACAATTTTCCCCGGAGATGCTTATACAGTTGATAGAAGTGTTATTGATGAAGCTTGTGACAAAATCTTAGTTGAATTAGATGAGAGAATTAAATATTTTAAAGCTAATAACCAATTAATTGAAGCTCAAAGAATCCAAGAAAGAGTTGAAAAAGATGTCGATAGCTTAAGAGAATTTGGTATGTGTCCTGGTATTGAAAACTATGCAAGATATATGGATAGAAGAGAAGCAGGCGAGCGTCCTTATACCTTATTAGACTATTTTAAAGGCAAAAAACCACTTTTATTTATTGATGAGAGTCACATGATGATTCCACAATTAAATGGAATGTATCATGGTGACTACTCACGTAAAAAAAACTTAGTTGATTATGGTTTTAGATTACCAAGTGCACTAGATAATAGACCACTGCTTTTTAAAGAATTTGAAAATGAATTTGATTTTCAAACTGTTTATATTTCAGCTACCCCTGATGATTATGAACTTGATAAAACTCATGGTTTATTAACCAAATTATTTGTTCGTCCTACTGGTCTTTTAGATCCTAAAATTGAAATTAGACTTTCTAAAAACCAAATTGAAGATATTTATGATGAATTGCAAAGACAAAAAGATAAAAATGAAAGAACTTTGATTTTAACTACAACTAAACGTTTAGCTGAAGAATTAAGTAGATACTTTTTAGAAAAAAATGAAAAAATTGCTTATATCCACTCAGAACATAATACTTTTGAACGGAATGAAATCTTAAGAAAACTTCGTAAAGGCGTTTACGACACTGTTATTGGAATTAACTTATTGCGTGAAGGTGTTGATTTACCTGAAGTAAGTTTAATTATGGTTTTAGATGCTGATAAAGAAGGCTTTATGCGTTCGACTAAAAGCTTAATCCAAATTTCAGGTCGGGCAGCTAGAAATGCTAATGGTCGAGTAATTTTCTATGCAGATAAAACTACTAAAAGTATGCAAGAGTGTATTGAAGATAATGAAATTAAAAGAAAGATGCAGATTGCTTATAATAAGAAACATAATATTACACCTAAAACCATTATTAAACCAATTGCTGAGCCAATTCATGGCGAAGGAATTGAAGGCGAAATTGAATTCTTTTTCAAAAACGAAAAACGAAATCAAACTCAAAGTGGCAAGAAAGCTAAAGAAGAACTTATTAAAAGATTACGTGAACAAATGAATCAAGCAGCTAAAGAATTGGACTATGAAAGAGCTATTGAATTGCGCGATATTATCCTAGAATTACAAGCTGGTAAAGATAAGAAAGTTGAGGAATAA", "aa": "MSIFKLISPYKPAGDQPEAIKELVENIKDNMQHQVLKGVTGSGKTFTIANVIKEFDRPVLVLSHNKTLASQLYSELKGFFPHNRVEYYVSYFDYYRPEAYIPSSDTYIDKQSQNNKDLDAMRMSAMNAVLTRNDTIIVASVSAIYGALNPSEYYASVFPIEVGMQIKRQEFFKKLVQRNYKRNQIDLELGTFSAKGDAVYIQPADSDEYQIRVDFFGDEIDSIATCDPITKEVIERFSHYTIFPGDAYTVDRSVIDEACDKILVELDERIKYFKANNQLIEAQRIQERVEKDVDSLREFGMCPGIENYARYMDRREAGERPYTLLDYFKGKKPLLFIDESHMMIPQLNGMYHGDYSRKKNLVDYGFRLPSALDNRPLLFKEFENEFDFQTVYISATPDDYELDKTHGLLTKLFVRPTGLLDPKIEIRLSKNQIEDIYDELQRQKDKNERTLILTTTKRLAEELSRYFLEKNEKIAYIHSEHNTFERNEILRKLRKGVYDTVIGINLLREGVDLPEVSLIMVLDADKEGFMRSTKSLIQISGRAARNANGRVIFYADKTTKSMQECIEDNEIKRKMQIAYNKKHNITPKTIIKPIAEPIHGEGIEGEIEFFFKNEKRNQTQSGKKAKEELIKRLREQMNQAAKELDYERAIELRDIILELQAGKDKKVEE", "aa_hexdigest": "dbfa3505caad1d745101853c8b8ae6bc", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C1E6", "ncbi_nrp_id": "WP_013354505.1", "uniref100_id": "UniRef100_C4XEN3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C1E6", "RefSeq:WP_013354505.1", "UniRef:UniRef100_C4XEN3" ] }, "ips": { "uniref100_id": "UniRef100_C4XEN3", "uniref90_id": "UniRef90_C4XEN3" }, "psc": { "uniref90_id": "UniRef90_C4XEN3", "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "ec_ids": [ "3.1.25.-" ], "uniref50_id": "UniRef50_Q8EUN2", "go_ids": [ "GO:0003677", "GO:0005515", "GO:0005524", "GO:0006289", "GO:0009380", "GO:0016787", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_Q8EUN2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8EUN2" ], "product": "UvrABC system protein B" }, "genes": [ "uvrB" ], "id": "OAIKOHHJFB_685", "locus": "GCA000209735_00661" }, { "type": "cds", "sequence": "contig_1", "start": 371178, "stop": 371420, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEN4", "UniRef:UniRef90_C4XEN4" ], "nt": "ATGAGAGTGACATTATTTGATTTAGTTAACAACTTAAAGTATTATTCTTCTTTAACTATTAACTTATATTCTATTAATAAAGCTATTAGACAACTATTAAAAATGATTCCAAATTTGAGTGCTAAAGAAAAAGAACTTTTAACTTTAGATTATTTAGGAACCGAGTTTAATAAATATTCGAGTGAGCTTTATAAACATGCAATTTGTATTGTTTATTTAGCTAATAAATATAAAGTTAATTGA", "aa": "MRVTLFDLVNNLKYYSSLTINLYSINKAIRQLLKMIPNLSAKEKELLTLDYLGTEFNKYSSELYKHAICIVYLANKYKVN", "aa_hexdigest": "da113e026a68a00f28314dbbeadff8ad", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 371172, "stop": 371684, "observations": { "mutation": [ 371418 ], "stop": [ 371418 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEN4", "query_cov": 1.0, "subject_cov": 0.47058823529411764, "identity": 1.0, "score": 156.0, "evalue": 3.67e-47, "gene_start": 1, "gene_end": 80, "reference_start": 3, "reference_end": 82, "reference_sequence": "MKMRVTLFDLVNNLKYYSSLTINLYSINKAIRQLLKMIPNLSAKEKELLTLDYLGTEFNKYSSELYKHAICIVYLANKYKVNWAVNFIKDLKLELNKVNDIEGFLDLVLTQKNQKLIKQKLSQIFKFVNLDGNVKDIIIIYDLICFIGLLVNLTNTEFELLPYDACNIIS" }, "paralog": false, "identity": 0.9882352941176471, "score": 329.0, "evalue": 9.64e-118, "description": "Internal stop codon at 371418. Nonsense mutation around 371418.", "psc": { "uniref90_id": "UniRef90_C4XEN4", "uniref50_id": "UniRef50_C4XEN4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEN4", "UniRef:UniRef50_C4XEN4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEN4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEN4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9398.959099999995, "isoelectric_point": 9.380746269226073 }, "id": "OAIKOHHJFB_686", "locus": "GCA000209735_00662" }, { "type": "cds", "sequence": "contig_1", "start": 371700, "stop": 372194, "strand": "+", "frame": 3, "gene": null, "product": "UvrABC system protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A848YIN1" ], "nt": "ATGTCAGCCAAAGAAGAAATTATAGTTAAAGGTGCTAGAGAACATAATTTAAAAAATGTTTCAGTAACTTTACCTAAAAATAAACTCATAGTTTTTACAGGGCTTTCAGGTAGTGGAAAGTCTTCTTTAGCATTCAATACTATTTATGAAGAAGGTAGAAGAAAATATGTTGATAGTTTAAGTAACTATGCTCGTTTATTTTTAGGTGGAACAAGTAAACCTGATGTTGATAGTATTGATGGGCTTAGTCCTTCTATTTCAATTGAACAAAAAACAACACATAATAACCCTCGTTCAACTGTTGGTACTGTTACTGAAATTTATGACTATTTTAGATTACTTTTTGCTCGTATTGGTAAACCATTTTGTCCTCACCATAATATTGAAATAACTAGTCAAACCAATAAAGATATTATTGATTCAGTTTTCAATTTAAAAGATGGATCAAAACTTTATATTTTAGCTCCTGTTGTTGATGGTGAAAAAGGAACCTGA", "aa": "MSAKEEIIVKGAREHNLKNVSVTLPKNKLIVFTGLSGSGKSSLAFNTIYEEGRRKYVDSLSNYARLFLGGTSKPDVDSIDGLSPSISIEQKTTHNNPRSTVGTVTEIYDYFRLLFARIGKPFCPHHNIEITSQTNKDIIDSVFNLKDGSKLYILAPVVDGEKGT", "aa_hexdigest": "9d7e153b83907ee5c6a8c1cceb991b4e", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_UPI0021D31D26", "query_cov": 0.9817073170731707, "subject_cov": 0.8944444444444445, "identity": 0.6459999999999999, "score": 218.0, "evalue": 6.86e-70, "valid": false, "uniref50_id": "UniRef50_A0A848YIN1" }, "pscc": { "uniref50_id": "UniRef50_A0A848YIN1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A848YIN1" ], "product": "UvrABC system protein A" }, "genes": [], "id": "OAIKOHHJFB_687", "locus": "GCA000209735_00663" }, { "type": "cds", "sequence": "contig_1", "start": 372327, "stop": 372596, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTTTAAATGAAGAAAATAGAAACAGAATTTCTGAAGCTTTAAGTGTTGCTGTGGAATATGGAAAAGGCATAGTTAAAGTTGAAACTACTGATGATGAAGTTAAAACATTTTCAAAATTACACTCTTGTGTTTATAAAGATTTTGAAATGCCTAAAATTGAAACTAAACTTTTCTCATTTAACTCGCCTTATGGTATGTGTCAAAATTGCAAAGGACTTGGAGTCGATTTAAAAGCTGACTTTGATGCTCTAACTCCAGAACCTTGA", "aa": "MVLNEENRNRISEALSVAVEYGKGIVKVETTDDEVKTFSKLHSCVYKDFEMPKIETKLFSFNSPYGMCQNCKGLGVDLKADFDALTPEP", "aa_hexdigest": "fc551f295422ab42edea8ee30980c0e5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10011.31079999999, "isoelectric_point": 4.910002326965332 }, "id": "OAIKOHHJFB_688", "locus": "GCA000209735_00664" }, { "type": "cds", "sequence": "contig_1", "start": 372906, "stop": 374522, "strand": "+", "frame": 3, "gene": null, "product": "UvrABC system protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P0N4" ], "nt": "ATGGGTACTTTTGAATGTTCAACTTGCCATGGAAGTAGATTAAATGAATATGCACTAGCAGTTAGAATTAATGGTTATAACATAGAAGACTTTAGTCGTATGTCAGTTGAAGATGCTTTGATTACTATCGAAAACTTAAAATTGAATGAAGAAGAGACTAAGATTTCTACTTTAATTATTAATGAATTAATTAACCGTTTAAGTTTTCTTAAAAATGTTGGACTTAACTATTTAACTTTAAATAGAAATGCCGAAACTTTAAGTGGTGGTGAAAGTCAAAGAATTAAATTAGCAACTCAAATTGGTTCTAATTTAACTGGTGTTTTATATGTTTTAGATGAACCATCAATTGGGCTTCATCAAAAAGATAATGACCGTTTAATTAAAACACTTAAAAGTATGGTTGAAATAGGTAATACTTTAATTGTTGTTGAACATGATGAAGAAACAATTAGATCAGCAGATTATATTGTTGATATTGGACCTAAGGCAGGAATTCATGGTGGTGAAATTATTGCTCAAGGAACTTTAGAAGACATTATTAAAGAACCAAAAAGTATCACTGGAAAATATTTAAGTGGTGAAATGAAAATCGAAGTACCAAGAGTTAGAAGAAGTGGAAATGGCAAAGTAGTTACTATTTTTCAAGCTGCAGAAAACAACTTAAAAAATATTGATGTCAAGTTTCCTTTAGGTAAATTTATAGGAGTTACAGGCTTAAGTGGCAGTGGTAAAAGTAGTCTTGTAAATGAAATCCTAGTTAAAGGTATCGAAAGCGTATTAGGCCATAAAACAGGCAAAATCGGAAAATTCAAGAGTATTAGTGGACTAAATCAAATTGATAAAATTGTGCCAATTAGCCAAAGCCCTATTGGCAGAACTCCTCGTTCTAATCCAGCAACTTATACCTCAGTTTTTGATGATATTAGAGAAATTTTTGCCTCAGTTGAAGAATCACGTGCTAGAGCTTATAGCCGTTCTCGTTTTAGTTTCAATGTGCCTGGCGGACGTTGTGAAAAATGTCAAGGAGATGGTTCAATTAAAATTGAAATGCACTTTTTACCTGATGTTTATGTACCTTGTGACGAGTGCGATGGTCAAAGATATAATCGTGAAACTTTAGAGATTAAATACAAAAATAAAAATATAGCTCAAGTCTTAGATATGACTGCTGATGAAGCACTTGAATTCTTTAGTACTAGAGCAAATATTAGAATTAAATTAGAAACTTTAGTTGCTGTTGGATTAGGTTATATTAAGCTTGGACAATCATCAACAACACTTAGTGGTGGCGAAGCTCAACGGGTAAAATTAGCTACTTATTTACAAAAGAAGGCGACAGGAAAAACCATTTATGTATTGGATGAACCTACTACAGGATTACATTCTTATGATGTTGCAAACTTATTAAAAGTTTTAAATAAAATTGTTGATAATGGTGACACAGTTGTTGTTATTGAACACAATTTAGATGTCATTAAATGTTGTGATTATATTATCGATCTTGGCCCTGAAGGTGGGGTAAATGGTGGCCGAGTTGTGGCTACTGGAACTCCTGAACAAGTGGCAGAAAATGAAAATAGTTACACAGGACAATACCTAAAAATGATTTTATAA", "aa": "MGTFECSTCHGSRLNEYALAVRINGYNIEDFSRMSVEDALITIENLKLNEEETKISTLIINELINRLSFLKNVGLNYLTLNRNAETLSGGESQRIKLATQIGSNLTGVLYVLDEPSIGLHQKDNDRLIKTLKSMVEIGNTLIVVEHDEETIRSADYIVDIGPKAGIHGGEIIAQGTLEDIIKEPKSITGKYLSGEMKIEVPRVRRSGNGKVVTIFQAAENNLKNIDVKFPLGKFIGVTGLSGSGKSSLVNEILVKGIESVLGHKTGKIGKFKSISGLNQIDKIVPISQSPIGRTPRSNPATYTSVFDDIREIFASVEESRARAYSRSRFSFNVPGGRCEKCQGDGSIKIEMHFLPDVYVPCDECDGQRYNRETLEIKYKNKNIAQVLDMTADEALEFFSTRANIRIKLETLVAVGLGYIKLGQSSTTLSGGEAQRVKLATYLQKKATGKTIYVLDEPTTGLHSYDVANLLKVLNKIVDNGDTVVVIEHNLDVIKCCDYIIDLGPEGGVNGGRVVATGTPEQVAENENSYTGQYLKMIL", "aa_hexdigest": "c9f1e1ab50166988e5f6cd6d899b9299", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D4XVF5", "query_cov": 1.0111524163568772, "subject_cov": 0.9963369963369964, "identity": 0.691, "score": 757.0, "evalue": 4.79e-271, "valid": false, "gene": "uvrA", "product": "excinuclease ABC subunit UvrA", "uniref50_id": "UniRef50_A0A3B0P0N4", "kegg_orthology_id": "K03701", "go_ids": [ "GO:0003677", "GO:0006289", "GO:0009380", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P0N4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P0N4" ], "product": "UvrABC system protein A" }, "genes": [], "id": "OAIKOHHJFB_689", "locus": "GCA000209735_00665" }, { "type": "cds", "sequence": "contig_1", "start": 374829, "stop": 374933, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGTTGCAACAAAAAAATATGTAAATAAATACCCTTACAAAGTTCGTAAATATGGACCTATTACCGAACGCCGTGATTACTCTGTAACTAAAATAACTTGA", "aa": "MDVATKKYVNKYPYKVRKYGPITERRDYSVTKIT", "aa_hexdigest": "5c7091208219028974a42cfdee7e2a1e", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4112.751300000001, "isoelectric_point": 9.917123222351073 }, "id": "OAIKOHHJFB_690", "locus": "GCA000209735_00666" }, { "type": "cds", "sequence": "contig_1", "start": 375078, "stop": 375245, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAATTCCTTCTAAAGAATATGAAGCTATTAGTGAAGCTAGAGTTAAAGGCACAAACTATGCTGCTAAACTTTATGCTAAATTTAGAGCTACAGCTACTGAAACAGGTATCGTTAAAGATGACAAAGTTCTTTTAGGTGAAATTCCACTTATGACTTCAGGTTGA", "aa": "MEIPSKEYEAISEARVKGTNYAAKLYAKFRATATETGIVKDDKVLLGEIPLMTSG", "aa_hexdigest": "fef94b549ee8ff0a83c844174dc2ced1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6006.8534, "isoelectric_point": 6.169042778015137 }, "id": "OAIKOHHJFB_691", "locus": "GCA000209735_00667" }, { "type": "cds", "sequence": "contig_1", "start": 375507, "stop": 376031, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATGAATCTTTTTGGTAAATCAGATGTTTTAAAAGAAACCATCAAAAAAGACAAAAAAGTTGATCCTGAGCTTGACCAAGACACAGTTATCAAAAACTGTCAAGAAGAATTATTTAGAGTTCTTCGTCGTGGTGACCGTATCACAGATGAATCAATTGCTAACTTGATTCCAAGCTTACTTTTCCACAGAAAACGTTACAGCTTAAGTGAAACTGGTCGTTATATGTTAAACAAAAAACTTAACTTAGTTGACCGTATTTCAGATACATTTTTAGGTGAAGACTTAGTTGTTATTAACAAAAATGGTGATGAAGAAAAAATTAAAAAAGGTACTTTGATTACACACAAAATTGCTTTATTAATTCAAAGCAACTTTGACAGTGGTAAATTACCTTCTGTTGAAATCGAAGGTGTAAGACCTGAAGTAGCATATGCTAAATTATTAAGTGATGCACAAAACAAATCACTTAAAAACAGAAACAAAATCATTATTTTAAAAATCTATCCAACCAAAAAATGA", "aa": "MIMNLFGKSDVLKETIKKDKKVDPELDQDTVIKNCQEELFRVLRRGDRITDESIANLIPSLLFHRKRYSLSETGRYMLNKKLNLVDRISDTFLGEDLVVINKNGDEEKIKKGTLITHKIALLIQSNFDSGKLPSVEIEGVRPEVAYAKLLSDAQNKSLKNRNKIIILKIYPTKK", "aa_hexdigest": "bfb120f8af0b7278200776ca119f93ac", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19969.0699, "isoelectric_point": 9.476546287536621 }, "id": "OAIKOHHJFB_692", "locus": "GCA000209735_00668" }, { "type": "cds", "sequence": "contig_1", "start": 376032, "stop": 377990, "strand": "+", "frame": 3, "gene": null, "product": "DNA-directed RNA polymerase subunit beta", "db_xrefs": [ "EC:2.7.7.6", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549", "KEGG:K03043", "SO:0001217", "UniRef:UniRef50_Q98Q23", "UniRef:UniRef90_C4XEN6" ], "nt": "ATGGATAAAGGTTTAACTCCAGTAAAAGTTATTGGAAATGACCCTAAATCAGTTGAACAACACTTATTAGTATCAGATATTATTGCTGCTGTAGGTTACTACTTCAACTTATTAGATGGCATTGGACAAGATGATGATCCTGACTCATTAACAAACAAACGTATTGTTGCAGTTGGTGAATTATTACAAAGCCAATTAAATGTCGGTTTAGTAAAACTTGAAAAAACTACACGTGAAAGAATGGGCGCTAAAGAACCTGACAAAGTAACACCTAAAAATGTTACAAACAATAAACTCATTTCAACACAAATGAAAACATTCTTTAACTCATCAAAACTTTCACAATTTATGGATCAAATTAATCCACTTGCTGAAATTTCAAATGAACGTCGTATCACTTCTCTAGGACCTGGTGGTCTTAACCGTGATACAGCTCAATTCGAAGTTCGTGACGTTCATGCAACTCACTATGGAAGAATTTGCCCTATTGAAACTCCTGAAGGACCTAACATTGGTCTTATCTTAAACTTTGCAACATATGCACAAGTTAATAAATATGGTTTTTTGCAAACTCCTTACTATAAAGTTGAAAATGGTGTAGTTGACTATTCAGAAGTTCATTATTTAACAGCTGCTGAAGAAATTGGATTTAATATTGCTCAATCAATTGTTAAAGTTGATGAAAACAATAAATTAGTTGATAAACAACTTACAATGCGTCACAATTACAACTATATTATTGGTAAACCTAAAGAAGTAGACTACATCGAAGTAGCTCCAAACCAAATGGTTTCAGTTGCTGCCGGATGTATTCCTTTCTTAGAAAATGACGATGCTAACCGTGCACTTATGGGTTCTAACATGCAACGTCAAGCAGTTCCTCTTTTAATTCCTGAAGCTCCTTTTGTTGCCACAGGTATTGAATCTGATATTGCTAAATATTCATCAGGTAACTTCGTTGCTAAAAATGATGGGCTTGTTGAATATGTTGATGGTAAGAAAATCAAAGTTAGAAATCAAAAAGGCACTTTAGATACTTACTATCTTAAAAACTTCCAACGTTCAAACCAAGATACAGTTGTGCACCAAAGACCTTTAGTTAAAGAAGGCCAAGAAATCAAAAAAGGTGATTTACTTGTTGATGGCTCAAGTTTCAAAAATGGTGAATTAGCATTAGGCAAAAACGTAGTTGTTGCCTTCACAACATATAAAGGATACAACTATGAGGATGCTGTTATTCTTAATGAACGTTTAGTTAAAGATGATGTCTTTACTTCAATTCATATTGAAGAACAAACAATTCAATTCAGAACTTCAAAAGCTGGAGATGATGAATTAACTTTAGATATTCCTAACGTTTCTAAATATGCAACACGTCATTTAGATGAACATGGAATTGTAAGAATTGGATCAGAAGTTGTTCCTGGAGATGTTTTAGTTGGTCGTGTAAGCCCTAAAGGTGATGACAACCCATCTCAAGAAGAAAAACTTCTTTCAGCTGTGCTTCAACAAAGACAATTAAATGTTAAAGATACTTCATTAAAAGTTAAAAATGGACACAATGGTACCGTTATTGGTGTTGATGTTTTAAGCCGTGAAAATGGTGATGCGTTAGAAGATGGAATTGAAAAAATCGTTAAGGTTTCAATTGCTATTAAACGTAAAATCAAAGTTGGTGACAAAATGTCAGGGCGTCACGGTAACAAAGGTGTTGTTTCAATCGTATTACCTGAAGAAGATATGCCTTATTTAGAAGATGGTACACCAGTTGATGTTATGCTTAACCCTCAAGGTGTTCCTTCACGTATGAACATTGGTCAAGTTCTTGAAATTCACTTAGGTATGGCTGCTAAAAAACTTGGATGTAAATTTGTAACTCCTACTTTTGATGGGGTTAAAAAAGAAGAAATTCAAGATGTTACTTTAGAAGCTGGTTTACCTTTAAGTGGAAAACAATGA", "aa": "MDKGLTPVKVIGNDPKSVEQHLLVSDIIAAVGYYFNLLDGIGQDDDPDSLTNKRIVAVGELLQSQLNVGLVKLEKTTRERMGAKEPDKVTPKNVTNNKLISTQMKTFFNSSKLSQFMDQINPLAEISNERRITSLGPGGLNRDTAQFEVRDVHATHYGRICPIETPEGPNIGLILNFATYAQVNKYGFLQTPYYKVENGVVDYSEVHYLTAAEEIGFNIAQSIVKVDENNKLVDKQLTMRHNYNYIIGKPKEVDYIEVAPNQMVSVAAGCIPFLENDDANRALMGSNMQRQAVPLLIPEAPFVATGIESDIAKYSSGNFVAKNDGLVEYVDGKKIKVRNQKGTLDTYYLKNFQRSNQDTVVHQRPLVKEGQEIKKGDLLVDGSSFKNGELALGKNVVVAFTTYKGYNYEDAVILNERLVKDDVFTSIHIEEQTIQFRTSKAGDDELTLDIPNVSKYATRHLDEHGIVRIGSEVVPGDVLVGRVSPKGDDNPSQEEKLLSAVLQQRQLNVKDTSLKVKNGHNGTVIGVDVLSRENGDALEDGIEKIVKVSIAIKRKIKVGDKMSGRHGNKGVVSIVLPEEDMPYLEDGTPVDVMLNPQGVPSRMNIGQVLEIHLGMAAKKLGCKFVTPTFDGVKKEEIQDVTLEAGLPLSGKQ", "aa_hexdigest": "f4a833893f9c61d3741c0dda25f49b89", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028FF9953", "query_cov": 0.8634969325153374, "subject_cov": 0.9825479930191972, "identity": 0.51, "score": 563.0, "evalue": 1.63e-192, "valid": false, "product": "DNA-directed RNA polymerase subunit beta", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_A0A7V5ZEA3", "kegg_orthology_id": "K03043", "go_ids": [ "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549" ] }, "genes": [], "truncated": "both", "pseudogene": { "start": 375732, "stop": 378293, "observations": { "mutation": [ 376029, 377988, 378183 ], "stop": [ 376029, 377988, 378183 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEN6", "query_cov": 1.0, "subject_cov": 0.5370675453047776, "identity": 1.0, "score": 1278.0, "evalue": 0.0, "gene_start": 1, "gene_end": 652, "reference_start": 411, "reference_end": 1062, "reference_sequence": "MMRKKEYYCMDVATKKYVNKYPYKVRKYGPITERRDYSVTKITWNVPDFLVTSKESFEWFKKTGIEESLREHYPITATNKKVSLEYIPNSADLEIPSKEYEAISEARVKGTNYAAKLYAKFRATATETGIVKDDKVLLGEIPLMTSGWSFIINGSEKVIVSQLIRSPGAYFGRGVRNKQSDDLFNKVEILPRIGSWVEISHKVTTKTPDSVKIKIDKNKNVNLVTFLGSLGLDRQMIMNLFGKSDVLKETIKKDKKVDPELDQDTVIKNCQEELFRVLRRGDRITDESIANLIPSLLFHRKRYSLSETGRYMLNKKLNLVDRISDTFLGEDLVVINKNGDEEKIKKGTLITHKIALLIQSNFDSGKLPSVEIEGVRPEVAYAKLLSDAQNKSLKNRNKIIILKIYPTKKWMDKGLTPVKVIGNDPKSVEQHLLVSDIIAAVGYYFNLLDGIGQDDDPDSLTNKRIVAVGELLQSQLNVGLVKLEKTTRERMGAKEPDKVTPKNVTNNKLISTQMKTFFNSSKLSQFMDQINPLAEISNERRITSLGPGGLNRDTAQFEVRDVHATHYGRICPIETPEGPNIGLILNFATYAQVNKYGFLQTPYYKVENGVVDYSEVHYLTAAEEIGFNIAQSIVKVDENNKLVDKQLTMRHNYNYIIGKPKEVDYIEVAPNQMVSVAAGCIPFLENDDANRALMGSNMQRQAVPLLIPEAPFVATGIESDIAKYSSGNFVAKNDGLVEYVDGKKIKVRNQKGTLDTYYLKNFQRSNQDTVVHQRPLVKEGQEIKKGDLLVDGSSFKNGELALGKNVVVAFTTYKGYNYEDAVILNERLVKDDVFTSIHIEEQTIQFRTSKAGDDELTLDIPNVSKYATRHLDEHGIVRIGSEVVPGDVLVGRVSPKGDDNPSQEEKLLSAVLQQRQLNVKDTSLKVKNGHNGTVIGVDVLSRENGDALEDGIEKIVKVSIAIKRKIKVGDKMSGRHGNKGVVSIVLPEEDMPYLEDGTPVDVMLNPQGVPSRMNIGQVLEIHLGMAAKKLGCKFVTPTFDGVKKEEIQDVTLEAGLPLSGKQWLIDPITGNKLDKPVSVGVMYMLKLNHMVDDKMHSRSVGPYSLITQQPLGGKSQNGGQRFGEMETWAIESYGATNVLQEILTYKSDDINGRNQLYAALASGRELPKPGVPESFNVLSYELKGLGMKLDLKKVKQDDNDFQQHFDIRGGDSNE" }, "paralog": false, "identity": 0.9964830011723329, "score": 1669.0, "evalue": 0.0, "description": "Internal stop codon at 376029, 377988, 378183. Nonsense mutation around 376029, 377988, 378183.", "psc": { "uniref90_id": "UniRef90_C4XEN6", "product": "DNA-directed RNA polymerase subunit beta", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_Q98Q23", "kegg_orthology_id": "K03043", "go_ids": [ "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEN6", "EC:2.7.7.6", "UniRef:UniRef50_Q98Q23", "KEGG:K03043", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q23", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q23" ], "product": "DNA-directed RNA polymerase subunit beta" } }, "id": "OAIKOHHJFB_693", "locus": "GCA000209735_00669" }, { "type": "cds", "sequence": "contig_1", "start": 378045, "stop": 378185, "strand": "+", "frame": 3, "gene": null, "product": "DNA-directed RNA polymerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920JZ79", "UniRef:UniRef90_A0A920JZ79" ], "nt": "ATGTACATGCTTAAACTTAACCACATGGTTGATGACAAGATGCACTCACGTTCAGTTGGACCTTACTCACTTATTACTCAACAACCTCTTGGAGGTAAGAGTCAAAACGGTGGTCAAAGATTTGGTGAAATGGAAACATGA", "aa": "MYMLKLNHMVDDKMHSRSVGPYSLITQQPLGGKSQNGGQRFGEMET", "aa_hexdigest": "6afa928b5687044909329d0b39d090c4", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 378045, "stop": 378235, "observations": { "deletion": [ 378216 ], "mutation": [ 378183 ], "stop": [ 378183 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A920JZ79", "query_cov": 0.9782608695652174, "subject_cov": 0.703125, "identity": 0.8220000000000001, "score": 84.0, "evalue": 1.72e-20, "gene_start": 1, "gene_end": 45, "reference_start": 1, "reference_end": 45, "reference_sequence": "MYIIKLNHLVDDKMHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALQAYGATNVPSRSVDS" }, "paralog": false, "identity": 0.746031746031746, "score": 100.0, "evalue": 1.29e-29, "description": "Internal stop codon at 378183. Frameshift due to deletion around 378216. Nonsense mutation around 378183.", "psc": { "uniref90_id": "UniRef90_A0A920JZ79", "product": "DNA-directed RNA polymerase", "uniref50_id": "UniRef50_A0A920JZ79", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A920JZ79", "UniRef:UniRef50_A0A920JZ79" ] }, "pscc": { "uniref50_id": "UniRef50_A0A920JZ79", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920JZ79" ], "product": "DNA-directed RNA polymerase" } }, "id": "OAIKOHHJFB_694", "locus": "GCA000209735_00670" }, { "type": "cds", "sequence": "contig_1", "start": 378228, "stop": 378446, "strand": "+", "frame": 3, "gene": null, "product": "DNA-directed RNA polymerase subunit beta", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P8L4" ], "nt": "TTGACATACAAATCAGATGACATTAATGGACGTAACCAACTATATGCTGCATTAGCTTCAGGACGTGAATTACCTAAACCAGGTGTCCCTGAATCATTTAACGTATTAAGCTATGAATTAAAGGGATTGGGAATGAAACTTGATCTTAAAAAAGTAAAACAAGACGATAATGATTTCCAACAACACTTCGACATTAGAGGAGGAGATTCAAATGAGTAA", "aa": "MTYKSDDINGRNQLYAALASGRELPKPGVPESFNVLSYELKGLGMKLDLKKVKQDDNDFQQHFDIRGGDSNE", "aa_hexdigest": "43872484d4cbd0d223949c4907053db8", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P8L4", "query_cov": 0.9305555555555556, "subject_cov": 0.8933333333333333, "identity": 0.537, "score": 74.7, "evalue": 3.08e-16, "valid": false, "product": "DNA-directed RNA polymerase subunit beta", "uniref50_id": "UniRef50_A0A3B0P8L4" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P8L4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P8L4" ], "product": "DNA-directed RNA polymerase subunit beta" }, "genes": [], "id": "OAIKOHHJFB_695", "locus": "GCA000209735_00671" }, { "type": "cds", "sequence": "contig_1", "start": 378439, "stop": 378534, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAACTTTATAGACAAAAACAAGTTAAATAAAGATCGTGAAATTGAAAAAATTACTCTTTCTTTAGCTACTAAAGATGACGTTCTTCAATGA", "aa": "MSNFIDKNKLNKDREIEKITLSLATKDDVLQ", "aa_hexdigest": "14a851522361cc5d273f42f8abbf6053", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3621.1214, "isoelectric_point": 6.026888465881346 }, "id": "OAIKOHHJFB_696", "locus": "GCA000209735_00672" }, { "type": "cds", "sequence": "contig_1", "start": 379099, "stop": 379515, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04997.17" ], "nt": "ATGGGTAAAGATTATGGTATCGACTTCTATGAATACAATGAAATTATTCACGAATACAGTGATGCTATTATCTCAACAGGTTCTAAAGCTATTGAATACTTATTAGAACATGTTGACTTACAAAAAGAAGCTCAATTAATTGAAGATGAAATTAAGAAAATCAATCGTTCAATTAAAGATAAAAATCACACAGGCACAAGAGGTCAAGAACGTTCAAAACTTTACAAACGTCTAGCTGTAATTAATGCCTTCATTAACTCAGGTCAAGATCCAAAAAGCATGCTTATTTATGATCTTCCAGTTATTCCAGCTGACTTACGTCCACTTGTTCAATTAGATGGTGGCCGTCACTCAACTAGTGATATTAATGAGCTTTATCGTCGTATTATTATTAGAAACAATCGTCTTAAAAAATGA", "aa": "MGKDYGIDFYEYNEIIHEYSDAIISTGSKAIEYLLEHVDLQKEAQLIEDEIKKINRSIKDKNHTGTRGQERSKLYKRLAVINAFINSGQDPKSMLIYDLPVIPADLRPLVQLDGGRHSTSDINELYRRIIIRNNRLKK", "aa_hexdigest": "c74c7a2c8f2075b913fff079790609c4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04997.17", "name": "RNA_pol_Rpb1_1", "length": 130, "aa_cov": 0.8623188405797102, "hmm_cov": 0.9615384615384616, "evalue": 1.0542394784950083e-22, "score": 76.9033432006836, "start": 20, "stop": 138 } ], "seq_stats": { "molecular_weight": 16003.04999999999, "isoelectric_point": 7.908546257019044 }, "id": "OAIKOHHJFB_697", "locus": "GCA000209735_00673" }, { "type": "cds", "sequence": "contig_1", "start": 379534, "stop": 379812, "strand": "+", "frame": 1, "gene": null, "product": "DNA-directed RNA polymerase subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B9S0R9" ], "nt": "ATGCTTATTAAGCAAAATGAATATCGTATGATTCAAGAAGCGGTTGACTCATTAATTGATAATGCACGTAAAAAACCAAATCCTGTTACTTCAAAAGATAGCCGTCCTCTTAAATCAATTTCAGACGCATTAACAGGTAAAAAAGGTCGTTTTCGTCAAAACTTACTTGGTAAACGTGTTGACTATTCAGGTCGTTCAGTTATTGTTGTTGGACCAGAATTAAAAATGCACCAAGTTGGTATTCCTCGTGAAATGGCTGCTAAATTATTTGAACCTTGA", "aa": "MLIKQNEYRMIQEAVDSLIDNARKKPNPVTSKDSRPLKSISDALTGKKGRFRQNLLGKRVDYSGRSVIVVGPELKMHQVGIPREMAAKLFEP", "aa_hexdigest": "cb37aaaa2b3672a596c9a0d2891c39d0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030FE3613", "query_cov": 0.8913043478260869, "subject_cov": 0.9647058823529412, "identity": 0.6459999999999999, "score": 109.0, "evalue": 1.75e-29, "valid": false, "uniref50_id": "UniRef50_A0A3B9S0R9" }, "pscc": { "uniref50_id": "UniRef50_A0A3B9S0R9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B9S0R9" ], "product": "DNA-directed RNA polymerase subunit" }, "genes": [], "id": "OAIKOHHJFB_698", "locus": "GCA000209735_00674" }, { "type": "cds", "sequence": "contig_1", "start": 379936, "stop": 380793, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00623.25", "PFAM:PF04983.23" ], "nt": "GTGCTTCTTAACCGTGCACCTACATTGCACCGTCTTTCAATTCAAGCTTATGAACCTGTTTTAATTCGTGGTAAAGCTATTAAACTTCACCCACTTTCATGTACTCCATTTAATGCTGACTTTGATGGTGACCAAATGGCAGTCCATGTTCCTATTAGTGATGAAGCAGTTAGAGAAGCCAAAGAATTGATGCTCGCTTCAAGAAATATTCTTGGGCCTAAAGATGGTGAACCTATTATCAACCCAGGTCAAGATATTATTTTAGGTCTTTATTACTTAACTCAAGAAGCTACAGATGATAAAGTAATTGGCGAAGGTCGTTATTTTGGATCATATGATGAAATGATGACAGCTTACGAAAATAAACTTGTTTCACTTCATGCTCGTGTTGTTTTACCAATTAGTGAAATCAATAAAAGAGTTATTCCAGCTGATGGTCAAGACCGTTACATAGTTTCAACTGTTGGTAAATTTATCTTCAACTCAATTTTCCCAGATAGCTTTGAATTTATCTTTGGAAAATATGTTGAAAGAAATATTAAAGAAGTGGATGGCAAAAAAGTAATCAGTGAAAAAGAAGTATTACACACTTCAGGTGATAAAAATCAACTTAAAAATTACTTATTACCTTATGGTATGAATTTCCCTGAAATTATTAAAAACATGCCACTTAACTTACCTTTAGGTAAAAAAGATATAGCTAAAATAGTACGTCGTATTTTTGACCGTTATGTAGCTATTGTTACAATGGAAGACTTAGCTGCTATTATTAGCGACTTAAATAGTGAAACAATTAACAACTTATTTGATAATTGTGCTAATTTAGTTGATTACAACAAAAACACTATTCCTGTATGA", "aa": "MLLNRAPTLHRLSIQAYEPVLIRGKAIKLHPLSCTPFNADFDGDQMAVHVPISDEAVREAKELMLASRNILGPKDGEPIINPGQDIILGLYYLTQEATDDKVIGEGRYFGSYDEMMTAYENKLVSLHARVVLPISEINKRVIPADGQDRYIVSTVGKFIFNSIFPDSFEFIFGKYVERNIKEVDGKKVISEKEVLHTSGDKNQLKNYLLPYGMNFPEIIKNMPLNLPLGKKDIAKIVRRIFDRYVAIVTMEDLAAIISDLNSETINNLFDNCANLVDYNKNTIPV", "aa_hexdigest": "4491e408f55da7532f23bf48b0a78893", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00623.25", "name": "RNA_pol_Rpb1_2", "length": 64, "aa_cov": 0.22456140350877193, "hmm_cov": 1.0, "evalue": 2.0110327689601944e-26, "score": 89.2982177734375, "start": 2, "stop": 65 }, { "id": "PF04983.23", "name": "RNA_pol_Rpb1_3", "length": 183, "aa_cov": 0.631578947368421, "hmm_cov": 0.73224043715847, "evalue": 4.9263475062335075e-23, "score": 77.87889862060547, "start": 69, "stop": 248 } ], "seq_stats": { "molecular_weight": 32178.772000000023, "isoelectric_point": 5.638109779357909 }, "id": "OAIKOHHJFB_699", "locus": "GCA000209735_00675" }, { "type": "cds", "sequence": "contig_1", "start": 380950, "stop": 381159, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTGATGTTTTAGACAAACTTAAAGCTTTAGGATATCATTATTCAACAATTTCAGGTACCACTATTTCAATTAGTGATGTTACTACTTTACCTTCAACAAAAGACAAAATTAAAGAAGGAGACAAATATATCGAACAATTAAGAGACTACTTTGAACAAGGCCTTTTAACTGATGATGAAAGATATGCTTTAACAATTCAAAAATGA", "aa": "MADVLDKLKALGYHYSTISGTTISISDVTTLPSTKDKIKEGDKYIEQLRDYFEQGLLTDDERYALTIQK", "aa_hexdigest": "748e7fd156653e0795e49424036d87ea", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7845.750899999997, "isoelectric_point": 4.898918724060058 }, "id": "OAIKOHHJFB_700", "locus": "GCA000209735_00676" }, { "type": "cds", "sequence": "contig_1", "start": 381232, "stop": 381999, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05000.22", "PFAM:PF04998.22" ], "nt": "ATGATGTTTACTTCTGGTGCTCGTGGTAACTCATCAAACTTTGTTCAATTAGCTGGTATGCGTGGACTTATGAATAACAACACTAAAATTCTTAAAGCTGACGCTGAAAATGAACGTGTTGTTCGTTCAACTGTTGAAATTCCAGTTAAATCATCATTCCTTGATGGTCTTACAGCTTATGAATTCTATTCATCAACCCACGGTGCTCGTAAAGGTCTTACCGATACAGCTCTTAACACAGCTAAATCAGGTTACTTAACTCGTCGTTTAGTTGATGTGGCTCAAGGTATCGTTCTTCGTGAAGATGATTGTGGAACTGACTATGGTTTCGAAGTTAAAGATATTCTTGATACAAAAACAAATACAATTATTGAATCACTTGGTGAAAGAATTGAAGGTCGTTTTTCAAACGTTCCTATTTATCATCCAAGAACTAAAGAAATGATTCTTGATGCAGATCAATTAATTACTCCTCAAATTGCTGAAGAAATTGTTAAAGCTGGTATTAAATCAGTTGAAATTCGTTCAGTACTTTCTTGCTATACTAAAAATGGTGTATGTAAAAAATGTTATGGTAAAGACTTAGCTCTTAACCGTGTAGTTAACATTGGTGAAGCTGTCGGAGTTGTTGCAGCTCAATCAATTGGTGAACCTGGTACACAGCTTACCATGCGTACATTCCATACTGGTGGTGTTGCTGGTGTTGAAGATATTACTGGTGGTTTTGGTCGTTTAATGGAATTAATCGATGCCTATGATTCACCATGA", "aa": "MMFTSGARGNSSNFVQLAGMRGLMNNNTKILKADAENERVVRSTVEIPVKSSFLDGLTAYEFYSSTHGARKGLTDTALNTAKSGYLTRRLVDVAQGIVLREDDCGTDYGFEVKDILDTKTNTIIESLGERIEGRFSNVPIYHPRTKEMILDADQLITPQIAEEIVKAGIKSVEIRSVLSCYTKNGVCKKCYGKDLALNRVVNIGEAVGVVAAQSIGEPGTQLTMRTFHTGGVAGVEDITGGFGRLMELIDAYDSP", "aa_hexdigest": "d497b97b7e240bf7c9ad743e60777c54", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05000.22", "name": "RNA_pol_Rpb1_4", "length": 56, "aa_cov": 0.20784313725490197, "hmm_cov": 0.9821428571428571, "evalue": 4.669889307377733e-12, "score": 41.91466522216797, "start": 1, "stop": 53 }, { "id": "PF04998.22", "name": "RNA_pol_Rpb1_5", "length": 195, "aa_cov": 0.7647058823529411, "hmm_cov": 0.5948717948717949, "evalue": 9.449907719511776e-46, "score": 152.45718383789062, "start": 56, "stop": 250 } ], "seq_stats": { "molecular_weight": 27683.191600000002, "isoelectric_point": 5.736384391784668 }, "id": "OAIKOHHJFB_701", "locus": "GCA000209735_00677" }, { "type": "cds", "sequence": "contig_1", "start": 382279, "stop": 382914, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGAAGTACAAAGACTTTATCGTCTTCAAGGTATCACAATTAGTGATAAATATATTGAAATCATTATTCGTCAAATGCTTTCAAAAATTGTTATTACTGATCCAGGAGATAGTAAATTCTTTAGTGGTAACTTAGTTGATATCTTTGCTTACCAACGTGAAAGTGCACGTTTAATTTCACAAGGTAAAAAGCCTCCTTTTGGTGAAGTTAAAATCAAAGGTGCAAAACAAACTCCTTTATTATCAGATTCATTCTTAGCTGCTGCTTCATACCAAGAAACTCCAAAAATTCTTGTTAATGCATCTATTGCTGCTCAATCAGATAAACTTGAAGGACTTAAAGAAAACATTATTTTAGGTCACAAAATTCCTGCTGGTACAAACTGTAATTTTGAAGAAAAAGGTAAATATGACATCCGTGATCCACGTTCATATTTCCAAAGTAAATTTGCAGAAAACTCAGATTTTCCAAATCCAAACTTTGAAGATAACTTAAATAATTTACAAAGTCAATTAGATGAATTCAACAGTGTTGATCCTGAAGAATTAGCAATAGAAGAAGAAATGGGAATCGAAGAAGTTTATGAAGATGATTACTACTCAGATTTAGATGAAAATAATGACAACAATTAA", "aa": "MKEVQRLYRLQGITISDKYIEIIIRQMLSKIVITDPGDSKFFSGNLVDIFAYQRESARLISQGKKPPFGEVKIKGAKQTPLLSDSFLAAASYQETPKILVNASIAAQSDKLEGLKENIILGHKIPAGTNCNFEEKGKYDIRDPRSYFQSKFAENSDFPNPNFEDNLNNLQSQLDEFNSVDPEELAIEEEMGIEEVYEDDYYSDLDENNDNN", "aa_hexdigest": "a21de706efa36cc86eee2f4bf5c11a92", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23991.430699999997, "isoelectric_point": 4.520939445495605 }, "id": "OAIKOHHJFB_702", "locus": "GCA000209735_00678" }, { "type": "cds", "sequence": "contig_1", "start": 383581, "stop": 384051, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTAAACTAAAACAAACTTTGATTATTTTATCAACCACTTTAGTTGGCACAATTGCTCTAGCAACTGGGCTTAGTTTCTTACCAACAAAAGACAAAACAATTCCTCGTGGTAAACAATCTTTCAATGATGACCATAAAAATTACAATGACCCTAATATTCCAAATTTACCTGGCAATAACGTTCCAACCAAAAACCCTAAACCTACATTAGTTTTAAAAAATCGACCTAAATCTGCAAATGCAATTACTAAAGAAAATAGCATAAAATATTTAGCTTTAGGCGACTCAATTTCAGCTGGATTTAATGCTCAACTTGATAAAGATTATCCAGGACAATTAGTTAATTCAAAAATTGAAGGTTTATCATTCCCAACTTATTTAGCTTCATTTTTCCAACAATTAGCAGACAAAAAATTAGAAAGTTTTGATAATAAAGCAGTTTCTGGAACAACCTTTAAACAATGA", "aa": "MKIKLKQTLIILSTTLVGTIALATGLSFLPTKDKTIPRGKQSFNDDHKNYNDPNIPNLPGNNVPTKNPKPTLVLKNRPKSANAITKENSIKYLALGDSISAGFNAQLDKDYPGQLVNSKIEGLSFPTYLASFFQQLADKKLESFDNKAVSGTTFKQ", "aa_hexdigest": "ff68db457853b9f942c62f73e6c18645", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17097.40229999998, "isoelectric_point": 9.726296806335448 }, "id": "OAIKOHHJFB_703", "locus": "GCA000209735_00679" }, { "type": "cds", "sequence": "contig_1", "start": 384100, "stop": 384600, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCAGAATTAAACAAATATTTTGGAACTGATTTAGTAAATATTAAAAATGATTTAATTGCTAAAATTAAAAATGCAAACTTAATAACAGTTACTTTAGGTGCTAATGATTTCTTAAAGGATTTATTATTTAAAATGGTAAGTAACATGCCAATTTTGGATTTAATTAAGCAAATTAAAAACAAAAATTTGGACTACAATATGTTAGTTTCACATTTTGATCAAGTTTTTAGAACACTTTTTGAACAACTTGAAAATCGTCAAAAACAATTTATTCAAACTATTAAAAGTCTAAATGATAAAGTTAATATTAATTTCATTGCTTATCCTTTACCATTAACTTTTATAATGGATTTAATTGATAAAGTGATTAACAAAGGCGATACAAATAATGCTTTAAGTATTGGTCAAACTATTTTAGATTTATTAAATAAAAAGCTTAAATATACTGCAATTAGTCAAAAAACATATTACATAAATCCATATCAAAGTAGCTTTTGA", "aa": "MAELNKYFGTDLVNIKNDLIAKIKNANLITVTLGANDFLKDLLFKMVSNMPILDLIKQIKNKNLDYNMLVSHFDQVFRTLFEQLENRQKQFIQTIKSLNDKVNINFIAYPLPLTFIMDLIDKVINKGDTNNALSIGQTILDLLNKKLKYTAISQKTYYINPYQSSF", "aa_hexdigest": "4313677772da1331c22646524de54cea", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19202.2308, "isoelectric_point": 9.280111122131348 }, "id": "OAIKOHHJFB_704", "locus": "GCA000209735_00680" }, { "type": "cds", "sequence": "contig_1", "start": 384979, "stop": 385080, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAATCAAAAATTTATGAAACTATTGATCCAAATAGAAAACTAGAAAAATTCATGCATCGTGATGACTATGCAAACCAAAAATCTTTACGTGACTGA", "aa": "MFKSKIYETIDPNRKLEKFMHRDDYANQKSLRD", "aa_hexdigest": "d0f0b5888402d395d3ba5483dc28b33d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4118.6532, "isoelectric_point": 9.222669792175292 }, "id": "OAIKOHHJFB_705", "locus": "GCA000209735_00681" }, { "type": "cds", "sequence": "contig_1", "start": 385360, "stop": 385905, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAATTAGTTAAATCATTCTACAACGAAAATATCAGCAAATATATTTCACAAAATGATTTAGCAAGTTTATTGAAATTGGTTTTAAATACTAAATCAATTAAATCAGCTGTTGGTGATTTAGTTGTTGAATTAATTAACAGTGCTCCAGAATATGCAAAATGTAAAAGCATCAATGAATTATTTAATGTATTTATTAGTAATAAAAATAACCAAAACACCATTAAAAAATTCATTGAATCAATTGTTAATGACTTATTAAGTCAAAACGAATTTAAACAAATTGTTTCGCGTCTTGCAAGTAAAATAATTAAACAATATCCAGAATTATTTAAAGATATAGATGAAAATAATTTAACCAATTTAATTTATGAATCATTATCACTTGGTGGTGAAATTAATAATGCTTTAGGGCTAGTGACGAAATTTGTCAACAGCCTTTTAAATGAGTTAACTACAACCAATATTAAAGAATTTAAATTTGATAATGTAATTAAAAATACATTAACTGAAATTAAAACATTATTTGACAAAGACAATTGA", "aa": "MLKLVKSFYNENISKYISQNDLASLLKLVLNTKSIKSAVGDLVVELINSAPEYAKCKSINELFNVFISNKNNQNTIKKFIESIVNDLLSQNEFKQIVSRLASKIIKQYPELFKDIDENNLTNLIYESLSLGGEINNALGLVTKFVNSLLNELTTTNIKEFKFDNVIKNTLTEIKTLFDKDN", "aa_hexdigest": "10cc769436aa87017ce8b826c6c254c1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20575.448400000016, "isoelectric_point": 7.697386360168458 }, "id": "OAIKOHHJFB_706", "locus": "GCA000209735_00682" }, { "type": "cds", "sequence": "contig_1", "start": 386137, "stop": 386520, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGCAATTTAATCTCACAACTTGTGAGTGATTTATTAAAAACAAATCAAACACAATTGATGGATGTAACAAATTTTTTTGATGCTATAAAAGTTATCTTTAAAAACTTTGTAAATACTTCAGCTTATGACTATATTTCACCTTTAATTAAGAAGGTATTTGAGTTTCCTGAAGCTAAAGACTTATTCAAAAAAGCAATGGCAAAAATACCAGCTCAAGAAGCTAAAAATCTTAATGACCGAGATGTAGCTGATATAGTAAGTTTTGTTCTAGATAACAACGAATTTAAAGACATCTTGCAAGATTTTTTAGTAAAAGGTATTTTTGGCGATGCAATAACAATTGAAAAATTAAAAGATGCAAATAACATTATTAAATCATGA", "aa": "MSNLISQLVSDLLKTNQTQLMDVTNFFDAIKVIFKNFVNTSAYDYISPLIKKVFEFPEAKDLFKKAMAKIPAQEAKNLNDRDVADIVSFVLDNNEFKDILQDFLVKGIFGDAITIEKLKDANNIIKS", "aa_hexdigest": "26b8481b3cb62cff42b8bd457e13f4a0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14425.519299999976, "isoelectric_point": 5.098309898376464 }, "id": "OAIKOHHJFB_707", "locus": "GCA000209735_00683" }, { "type": "cds", "sequence": "contig_1", "start": 386680, "stop": 386868, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAACTTTATTGGTGACTTCTTAACAATTATTCCTTCTTTAGATGAAGAATTTAGATTTATAAAAGATGTCTTAACCAAAATATTTAATGAATTAAAAGCAACCGGTAAAGCAATTGACTTTAAAAAAGTCATGAGCGAATTTGCTAAATGTATTAAAAATAAATTATTTAACAAGAATGATTGA", "aa": "MENFIGDFLTIIPSLDEEFRFIKDVLTKIFNELKATGKAIDFKKVMSEFAKCIKNKLFNKND", "aa_hexdigest": "0255e34fa769216ded9bbcf6fc28bc14", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7248.440299999999, "isoelectric_point": 7.8331182479858406 }, "id": "OAIKOHHJFB_708", "locus": "GCA000209735_00684" }, { "type": "cds", "sequence": "contig_1", "start": 386917, "stop": 387819, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09204.15" ], "nt": "ATGAATAAGCATCAAGCATTTTTAAGAAAATTTGTTAAAAATATTCTTGTTTATGTCAGTGAAGATATAGCATTAGGTAAAAAACTATTTAAAAGCTTATCTGAAAAAGCACACAAAATAATTGATAAACACATTGATGAAAATGACTTTAACAATATGTTTATTGACTTATTCCAACGCACTGAAGGTCTTCAAAGAATTATAGATAACATTCTTGATTCATTATTTAGCGATATTGATTCATATCAAAATGCAAATAGCTTAATTGACATTCTTAAAATTTATGTAATGCCTCAAGATCGTGCTGATCGTTTAGCTAAAAACTTAGAAGATGTTCTAAAAGAATTCTTGAAAAATGAAGCTCTTCATAAATTATTAAGAGGTCTATTTGAAGAAAATGTTGCTCCTTATGGAATTGATCCTAAGAGCCCAGGTAATAAAGCTTTAGTTGATGATTTATTAGCAAACTTGCATACATTCTTTGTTGATTTAAAAATATTGCCTAACTTAGCTAACTCAATGGTTGACAGCTTCAAAAAATACAATAACTTTAATGAAATTATTAAAAATATTGAACCGATTTTAGTCAATGGACTTAAATTAAAAGAATACTCATTTGTGGCTACAATTCTTAAAAATAATATTGTTAATAAACACAAAGAATCAATTAGAGATGATATCAACAAAGTTATCGCAGGTATCACTTCAAAAATTAATTTAGTTAACAAATTTATCGATGACTTTAATTTAATTAAACCTATTAAAGATATGGGCCTAAGTGAAACAAAAGCACGTGATTTCTTAACTCAAGTTATTCAGTCGCAAAACTTAAAAACTATAATAGATGTCTTTGTTAAAGAGCTTTTAGACAAAAACTTAGAATATGCTCAATTAAATTCATGA", "aa": "MNKHQAFLRKFVKNILVYVSEDIALGKKLFKSLSEKAHKIIDKHIDENDFNNMFIDLFQRTEGLQRIIDNILDSLFSDIDSYQNANSLIDILKIYVMPQDRADRLAKNLEDVLKEFLKNEALHKLLRGLFEENVAPYGIDPKSPGNKALVDDLLANLHTFFVDLKILPNLANSMVDSFKKYNNFNEIIKNIEPILVNGLKLKEYSFVATILKNNIVNKHKESIRDDINKVIAGITSKINLVNKFIDDFNLIKPIKDMGLSETKARDFLTQVIQSQNLKTIIDVFVKELLDKNLEYAQLNS", "aa_hexdigest": "43ea93ea515c6d8e67721c724751e3c5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09204.15", "name": "Colicin_immun", "length": 54, "aa_cov": 0.16, "hmm_cov": 1.0, "evalue": 1.5422678622244196e-06, "score": 24.79534912109375, "start": 37, "stop": 84 } ], "seq_stats": { "molecular_weight": 34552.618900000045, "isoelectric_point": 7.029585075378416 }, "id": "OAIKOHHJFB_709", "locus": "GCA000209735_00685" }, { "type": "cds", "sequence": "contig_1", "start": 387841, "stop": 387957, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTCACCAAGCGCATTAATAATTAAAGGAACAATTAAACAATACATAACTGAATTATTTGAAAAGAATGAAGAAGTAGCTTTTACAATTGGTAAACTTATAGCTAATGGTTGA", "aa": "MSSPSALIIKGTIKQYITELFEKNEEVAFTIGKLIANG", "aa_hexdigest": "02765bf2b02be693f886f10db481de01", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4155.807500000001, "isoelectric_point": 6.049339866638183 }, "id": "OAIKOHHJFB_710", "locus": "GCA000209735_00686" }, { "type": "cds", "sequence": "contig_1", "start": 388084, "stop": 388530, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGAGAATTAATACCTATGGCTTTGAAAAATTAATTGATAACTTAAGAGATGCTGCAAAAGAAGGAGCACTTAAGTTTATTAGCGAAGGTGGCAAAGTTAGATTATCAAAAATATTTGACCAAATACCTTTAATTAGAGATTTACTTGCTAAAGTACCTCAAAAAGAATTTTCAGATTTTCTTAACTTAGCATTTAGTTCAACTCCTAAAAATGATACAGAAGGATTATTCAAAGTGATGTTTAATTCTGATAAAGGAACATCTAAATTTGAAGTTGGTATTGGAGCAAGCGGAATTATCGATATTCTTAAAGGTAAAGTTGGTGATTTAGTTTCAGCTATTGCTTCACCTCTTGTTAAAGAATTCTTTAAACAAGCAAGTGAACAAAATTATAGTGATCATAATACATTAAGAAAAACTAATACAGGCTTCCAAGCTTTATGA", "aa": "MKRINTYGFEKLIDNLRDAAKEGALKFISEGGKVRLSKIFDQIPLIRDLLAKVPQKEFSDFLNLAFSSTPKNDTEGLFKVMFNSDKGTSKFEVGIGASGIIDILKGKVGDLVSAIASPLVKEFFKQASEQNYSDHNTLRKTNTGFQAL", "aa_hexdigest": "53a8667c8547f80e4d9fd11db7de92c7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16379.613899999986, "isoelectric_point": 9.272439384460448 }, "id": "OAIKOHHJFB_711", "locus": "GCA000209735_00687" }, { "type": "cds", "sequence": "contig_1", "start": 388624, "stop": 388776, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGGTTTTGTCAAAGCATTCAAAGATAACGTAAAAGACTATAAAACAGCCTTATTGGCAAAATATGGTGATAAAAGTTTAATAGGATTTAATAATAGTTTTGACCCTCTTGATTATTACTTAAGTGGTATGACAACAGTAAATTATTGA", "aa": "MDGFVKAFKDNVKDYKTALLAKYGDKSLIGFNNSFDPLDYYLSGMTTVNY", "aa_hexdigest": "5e365e2345442631321330a147c5852a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5680.373400000001, "isoelectric_point": 5.937992286682129 }, "id": "OAIKOHHJFB_712", "locus": "GCA000209735_00688" }, { "type": "cds", "sequence": "contig_1", "start": 388966, "stop": 389247, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAAAAGAATTTAAAAAGAGATTAATTGAATACTGCGAAACGGAAGGATTGAGCAGATATGAAGAAAATGTAGCTCATGCATTAGTGAAAAATATAAATTCAAAAAACTTTGAAGTTAACTATGACAATTTTGGATCTTTAATTTTGCATAAAAAATCAAAAGTTAAAGATGCTCCTAAATTTATGATAGCTGCTCATATGGATGAAGTTGGTTATTTAGTACGTCAAATTCATGAAGATGGACAAATTCTTTTAAGCTCAGTTGGTGGAATTTGA", "aa": "MNKKEFKKRLIEYCETEGLSRYEENVAHALVKNINSKNFEVNYDNFGSLILHKKSKVKDAPKFMIAAHMDEVGYLVRQIHEDGQILLSSVGGI", "aa_hexdigest": "9358c8ae01ae0baa5ada4f3e8ff7af1a", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10670.157199999998, "isoelectric_point": 7.862451362609863 }, "id": "OAIKOHHJFB_713", "locus": "GCA000209735_00689" }, { "type": "cds", "sequence": "contig_1", "start": 389338, "stop": 390051, "strand": "+", "frame": 1, "gene": "ysdC", "product": "Aminopeptidase ysdC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P1S5" ], "nt": "ATGGAAGCAGAAGCTAGAAATAGAGCGGTAACAAATAAAGAAATTTATGCTGATTTTGGCTTTAAAAACAAAGAAGAGGCATTAAAGAAAAATGTTCAAATTGGTGATCGTGTTTTAATGTCGGGTGAAACAATCAATTTTGACAATGAAAACTTAGTTGGAGCTAAAGCTATGGACAATAGAGCTGGAGTTACAACACTAGAATATATAGCTCATCAATTAAAAGATTTAGACTTAGCAGTTGATTTATATTTAGTTGGAACAGTTCAAGAAGAAGTAGGTACTAGAGGTGCTAAAACCTCAGTTACTGTTATTGATCCTCAAATTGCAATAGCACTAGATACAACCAGCACTCATGATACTATTGGTACTATTGCGGGTACTACCGCACTAGGTAAAGGCGCAGCCATTAGAATCATGGATGGAGCTATGATGGCTAATCCAAGACTAGTTGAATTAATGTGTAAAATAGGCAGAAAAGAAAAAATCAAACACTACAAATTTGTTGCTATGGGTGGCGGTACTGATGCTAGTGCGCTTCAATATGCTAAAGGTGGAGCAGCCACTTTAACTATCTCTTTAGCTCAAAGATATCTTCATTCTCCAATTGGAGTTTGTGACATAGAAGACTTGATGGCAGCTGGTGATTTAATTGTTGAAACAGTTAAAGCAATTAATCCTAAAGTTTACGATCAAGAAATTAAATATCATTAA", "aa": "MEAEARNRAVTNKEIYADFGFKNKEEALKKNVQIGDRVLMSGETINFDNENLVGAKAMDNRAGVTTLEYIAHQLKDLDLAVDLYLVGTVQEEVGTRGAKTSVTVIDPQIAIALDTTSTHDTIGTIAGTTALGKGAAIRIMDGAMMANPRLVELMCKIGRKEKIKHYKFVAMGGGTDASALQYAKGGAATLTISLAQRYLHSPIGVCDIEDLMAAGDLIVETVKAINPKVYDQEIKYH", "aa_hexdigest": "2bf573c54280bf9338c038ebe2726a29", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FE1AB9A", "query_cov": 0.8649789029535865, "subject_cov": 0.9715639810426541, "identity": 0.649, "score": 255.0, "evalue": 3.94e-83, "valid": false, "product": "M42 glutamyl aminopeptidase", "uniref50_id": "UniRef50_A0A3B0P1S5" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P1S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P1S5" ], "product": "Aminopeptidase ysdC" }, "genes": [ "ysdC" ], "id": "OAIKOHHJFB_714", "locus": "GCA000209735_00690" }, { "type": "cds", "sequence": "contig_1", "start": 390154, "stop": 390354, "strand": "+", "frame": 1, "gene": null, "product": "putative protein family (UPF0154)", "db_xrefs": [ "KEGG:K09976", "SO:0001217", "UniRef:UniRef50_Q98R64", "UniRef:UniRef90_A0A3P8KMY9" ], "nt": "ATGGCAATCTTATTCTTTTTAATGGGTGGTTTTATTGCTTTTATCGTTTCAAAGAAAATGTTCGAAAAACAAATTAAAGAAAACCCTCCTATTACTGAAAAAATGATTAGAGCTATGTTCTTACAAATGGGAAGAAAAGCTTCAGAATCACAAATTAAAGCTGTTATGCGTTCAATGACAAATGCTAAAGATGAAAAATAA", "aa": "MAILFFLMGGFIAFIVSKKMFEKQIKENPPITEKMIRAMFLQMGRKASESQIKAVMRSMTNAKDEK", "aa_hexdigest": "6a8e6265ae53ebfde947e8b0bc752ba5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A3P8KMY9", "query_cov": 0.9848484848484849, "subject_cov": 0.8125, "identity": 0.9229999999999999, "score": 114.0, "evalue": 7.78e-32, "valid": true, "product": "Uncharacterised protein family (UPF0154)", "uniref50_id": "UniRef50_Q98R64", "kegg_orthology_id": "K09976" }, "pscc": { "uniref50_id": "UniRef50_Q98R64", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98R64" ], "product": "UPF0154 protein MYPU_1460" }, "genes": [], "id": "OAIKOHHJFB_715", "locus": "GCA000209735_00691" }, { "type": "cds", "sequence": "contig_1", "start": 390656, "stop": 391402, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAGTTATCCTAGAACTGATAGTACTAGATTGAGTCAACATTTCTTAAATATTGCTAGAAGTTATATTAAAAATAAATTCGGTGAAGAATATATTCTTCAAGAAATCAAAGGCTTTGCAGGCGATCAAGATGCCCATGAAGCTATTAGACCAACTGATATAAACTTACTGCCTGAAGTTGCTAAAGCTAAATTTAATTTAGATAATAGTGAATACATTGTTTATAAGCTAATTTATGAACACACACTTATGTCTTTAATTAATCCACCAATTAAAGCTAATAAAACTTATACCTTTAAAAAAAATAAGTTAACTTTTAAAGCTAATGTAAGTAAAACCATATTCCCAGGTTACTATCTTGTTAAAAATGAAAATGAAGATATTGAAGATCCTAATTATCAAATACAACAAAAAGTGGATGTTTTAGAATATCAAATAACAAATCATGAAACTAAACCACCAGCTCGTTACACTGAAGGTTCATTAATTGAAGCTTTAGATAATATTAAAGTTGGACGTCCTTCAACTTTTGCAACTACTGTTGCAGTAAACTTGGAAAGAAAATATTTTGAAAATATTGATAAAAGTTTACATCATACAGAATTTGGCCGTTTAGTTTTAAGTAAATTATTAGATGGTTTTCCAGATATTATTAATGAAGGCTATACAGCTGAAGTTGAAGAACAATTAGACTTAATTGCTTCAAATAAAACTACTGTTCAACCTATTATGTATGATTTTTGA", "aa": "MISYPRTDSTRLSQHFLNIARSYIKNKFGEEYILQEIKGFAGDQDAHEAIRPTDINLLPEVAKAKFNLDNSEYIVYKLIYEHTLMSLINPPIKANKTYTFKKNKLTFKANVSKTIFPGYYLVKNENEDIEDPNYQIQQKVDVLEYQITNHETKPPARYTEGSLIEALDNIKVGRPSTFATTVAVNLERKYFENIDKSLHHTEFGRLVLSKLLDGFPDIINEGYTAEVEEQLDLIASNKTTVQPIMYDF", "aa_hexdigest": "28d32df3c851966589cfdf89fb77ea47", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28626.092700000023, "isoelectric_point": 5.59650363922119 }, "id": "OAIKOHHJFB_716", "locus": "GCA000209735_00692" }, { "type": "cds", "sequence": "contig_1", "start": 391613, "stop": 391852, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGATAGAATTATTGGTTTCAAATTAAGCCAATTAATGCAAAAGAAAATTTCAAATAGTCCTCGTACTCCAAGTGCAGGACGTGTACAATCAATTGCTCTTAAATTAGTAGTTGATCGTGAAGATGAAATTAATGCATTTGTGCCTCGTGGTTATCACAAAGTTGAAGCTAAATTAAATGAAACATTAAGTGCTTCTATTTATATAGATAAACATCCAGCAGCAGAAAAAAACTGA", "aa": "MLDRIIGFKLSQLMQKKISNSPRTPSAGRVQSIALKLVVDREDEINAFVPRGYHKVEAKLNETLSASIYIDKHPAAEKN", "aa_hexdigest": "3c96d2e6c57863e33521765f8e9558d3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8877.174699999994, "isoelectric_point": 9.628240394592286 }, "id": "OAIKOHHJFB_717", "locus": "GCA000209735_00693" }, { "type": "cds", "sequence": "contig_1", "start": 392138, "stop": 392293, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATTTAGTTATAGTGGAATCTCCAAACAAAGTTGAGACAATTAAAAAATATTTAGGAAGCGATTATGATGTGATTGCTTCTGTTGGTCATATCGCTATTATGAGTACTAGGAAAGGCGAACAAGGTTTAGGAATTGATTTAGAAAATTGA", "aa": "MNNLVIVESPNKVETIKKYLGSDYDVIASVGHIAIMSTRKGEQGLGIDLEN", "aa_hexdigest": "9faf956af32f225ce53d04ce4d2af35a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5561.301199999999, "isoelectric_point": 5.045847511291503 }, "id": "OAIKOHHJFB_718", "locus": "GCA000209735_00694" }, { "type": "cds", "sequence": "contig_1", "start": 392657, "stop": 392899, "strand": "+", "frame": 2, "gene": "rpsF", "product": "30S ribosomal protein S6", "db_xrefs": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "SO:0001217", "UniRef:UniRef50_C4XEP2", "UniRef:UniRef90_C4XEP2" ], "nt": "ATGCACAAATATGAAATTATGATGATACTTGATCCTAAGGCAGATGCCAAGAAGAGCATTGACTTAGTTGAAAGTATTTTCAAAAAATCAAATGTTAAAAAAGCTGAAAAGCTTGAAAGAACTGACTTAGCATATCCAATCAAAGGATCATTAAAAGCTCAATACTTACTTTTTGAAATTGAAGCTGAAAGTCACTTAATTGCTGAATTAACTCGTCGTGTTAATATCTCAAAAGATATTTGA", "aa": "MHKYEIMMILDPKADAKKSIDLVESIFKKSNVKKAEKLERTDLAYPIKGSLKAQYLLFEIEAESHLIAELTRRVNISKDI", "aa_hexdigest": "acdce77b6e5843fd69c6a76237c7d384", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "rpsF" ], "truncated": "3-prime", "pseudogene": { "start": 392657, "stop": 393130, "observations": { "mutation": [ 392897 ], "stop": [ 392897 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEP2", "query_cov": 1.0, "subject_cov": 0.5095541401273885, "identity": 1.0, "score": 152.0, "evalue": 1.16e-45, "gene_start": 1, "gene_end": 80, "reference_start": 1, "reference_end": 80, "reference_sequence": "MHKYEIMMILDPKADAKKSIDLVESIFKKSNVKKAEKLERTDLAYPIKGSLKAQYLLFEIEAESHLIAELTRRVNISKDIWRHLAINLDSERGYGKVKKESHRAKYEEKKEFKKSNKSAEACEKVAEEKAAKKPRSAKKESQEKEVKPRQSKAKKVE" }, "paralog": false, "identity": 0.9681528662420382, "score": 247.0, "evalue": 1.47e-85, "description": "Internal stop codon at 392897. Nonsense mutation around 392897.", "psc": { "uniref90_id": "UniRef90_C4XEP2", "gene": "rpsF", "product": "30S ribosomal protein S6", "uniref50_id": "UniRef50_C4XEP2", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEP2", "UniRef:UniRef50_C4XEP2", "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEP2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEP2" ], "product": "Small ribosomal subunit protein bS6" } }, "id": "OAIKOHHJFB_719", "locus": "GCA000209735_00695" }, { "type": "cds", "sequence": "contig_1", "start": 393158, "stop": 393307, "strand": "+", "frame": 2, "gene": null, "product": "Single strand binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEY7", "UniRef:UniRef90_A0A3P8MEY7" ], "nt": "ATGAACAAAGTATTACTTGTAGGTCGTATTTCAAATGATATAAGAATGTACAGAACAAATAGTGGCATTTCATATGCTAGAACTTCTATAGCAGTAAATCGTCGTTCTTCAAATGACAAAATTACTGATTTTATTCCTATTGTTGCATGA", "aa": "MNKVLLVGRISNDIRMYRTNSGISYARTSIAVNRRSSNDKITDFIPIVA", "aa_hexdigest": "624a0c9ac922e317e98f68c319adc8e4", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 393158, "stop": 393333, "observations": { "deletion": [ 393293 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8MEY7", "query_cov": 0.9183673469387755, "subject_cov": 0.7758620689655172, "identity": 0.9109999999999999, "score": 85.1, "evalue": 5.86e-21, "gene_start": 1, "gene_end": 45, "reference_start": 1, "reference_end": 45, "reference_sequence": "MNKVLLVGRISNEIRMYRTNSGIAYARTSVAVNRRSSNDKITDFVSYCRMKKFSRISK" }, "paralog": false, "identity": 0.896551724137931, "score": 101.0, "evalue": 4.26e-30, "description": "Frameshift due to deletion around 393293.", "psc": { "uniref90_id": "UniRef90_A0A3P8MEY7", "product": "Single strand binding protein", "uniref50_id": "UniRef50_A0A3P8MEY7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8MEY7", "UniRef:UniRef50_A0A3P8MEY7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MEY7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEY7" ], "product": "Single strand binding protein" } }, "id": "OAIKOHHJFB_720", "locus": "GCA000209735_00696" }, { "type": "cds", "sequence": "contig_1", "start": 393334, "stop": 393486, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTGTTCTTTGTTCTCTTACTGAACGAGGTTCAAGTAATGAAATATTGTCAACTGTAACTTCATATGATCAAACTGTTCCATTTTGTCCTTCATATTGTGATGAAGTAAATGAACCTTCAATTGAAACAAGAGCTCCTTTTGAAGCATAG", "aa": "MPVLCSLTERGSSNEILSTVTSYDQTVPFCPSYCDEVNEPSIETRAPFEA", "aa_hexdigest": "b4499a9fb398002494902aaefd897972", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5515.054299999999, "isoelectric_point": 4.0500284194946286 }, "id": "OAIKOHHJFB_721", "locus": "GCA000209735_00697" }, { "type": "cds", "sequence": "contig_1", "start": 393709, "stop": 393963, "strand": "+", "frame": 1, "gene": "rpsR", "product": "30S ribosomal protein S18", "db_xrefs": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "KEGG:K02963", "RefSeq:WP_013354516.1", "SO:0001217", "UniParc:UPI0001E3308C", "UniRef:UniRef100_C4XEP4", "UniRef:UniRef50_A5IY67", "UniRef:UniRef90_C4XEP4" ], "nt": "ATGGTATTTAATAACAAAAAGAAAAATAACTTTAGACGTCGTAGATGTGAAACTTGTGATCAAAAATTAACTTATGTTGATTACAAAGATGTTGAATTCTTAAGCAAATTTATTACAGCAACAGGTCAAATTAAACCTCACTCAGCTACTGGTGCATGTGCAAAAGATCAAAGAAAAATTGCAAATGCAATCAAAAGAGCTCGTTTTATGGCACTTATTCCTTATTCAAAAGATCGTGTTCGTTTAGTTAAATAA", "aa": "MVFNNKKKNNFRRRRCETCDQKLTYVDYKDVEFLSKFITATGQIKPHSATGACAKDQRKIANAIKRARFMALIPYSKDRVRLVK", "aa_hexdigest": "bb4e0704f05956295c561802130d9245", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E3308C", "ncbi_nrp_id": "WP_013354516.1", "uniref100_id": "UniRef100_C4XEP4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3308C", "RefSeq:WP_013354516.1", "UniRef:UniRef100_C4XEP4" ] }, "ips": { "uniref100_id": "UniRef100_C4XEP4", "uniref90_id": "UniRef90_C4XEP4" }, "psc": { "uniref90_id": "UniRef90_C4XEP4", "gene": "rpsR", "product": "30S ribosomal protein S18", "uniref50_id": "UniRef50_A5IY67", "kegg_orthology_id": "K02963", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_A5IY67", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IY67" ], "product": "Small ribosomal subunit protein bS18" }, "genes": [ "rpsR" ], "id": "OAIKOHHJFB_722", "locus": "GCA000209735_00698" }, { "type": "cds", "sequence": "contig_1", "start": 394056, "stop": 394226, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTTGAAAAAATATTTATTTGCACAACAGACACAGTAACAGGCATTGGCGGACCTATTTGCCAAGAAACCTTAGATGCTCTTTATTATCTTAAAAAAAGACCTAAAAGTAAAAAAATAATGATTTTAGTTGCCTCATTAAAACAAGCTCAAAGTTTTAAAGAATGA", "aa": "MNFEKIFICTTDTVTGIGGPICQETLDALYYLKKRPKSKKIMILVASLKQAQSFKE", "aa_hexdigest": "b85bc97ab39d8c249103dfa7341fc723", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6340.497000000001, "isoelectric_point": 9.301772499084471 }, "id": "OAIKOHHJFB_723", "locus": "GCA000209735_00699" }, { "type": "cds", "sequence": "contig_1", "start": 394305, "stop": 394502, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAATAACAAAAAATTATTAGAATTTTTAGAAAAAAATGGACCTATGTATGTAACAAGTGCTAACGTCTCAACTCAAGAACCGATTGATATTAAAGATGCTCATAAAGTTTTTCCAGAAGTTAAAAATGTTTATAACTTTGGAAAAGGCAAAGGTGTAGCTAGTCATATTATTGATGCTCAAAGTAATGAATGA", "aa": "MPNNKKLLEFLEKNGPMYVTSANVSTQEPIDIKDAHKVFPEVKNVYNFGKGKGVASHIIDAQSNE", "aa_hexdigest": "21ce2223b919c584756e23f5caaa5add", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7232.124700000004, "isoelectric_point": 6.7218019485473635 }, "id": "OAIKOHHJFB_724", "locus": "GCA000209735_00700" }, { "type": "cds", "sequence": "contig_1", "start": 394536, "stop": 395678, "strand": "-", "frame": 3, "gene": null, "product": "trigger factor-related chaperone", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEP6", "UniRef:UniRef90_C4XEP6" ], "nt": "ATGGAAAGCATTAAAGAAGGCAAGAAAATTTCACCTGACCAATTAATTGAAGAAGCTTTTAAATCAGATACAAATAAAATAGTTGAATCAATGGGTAAAGAATTAAAAGAAAAATATCTAAATTGTTTAGCTTTTTCGCCTATTGTTGTTGTCGAAGATATTACTAAAGATAAAGCTAAAGCTGTTGTTACAATTGTAATTTATCCAAAAGCTGAATTCGATAAAATTGACATAAATATTAAACCAGATGGTAAATATGAAATGGTGACAAAAGGTGCTATTGATGAAATTTACAAAAAATTTGTTGATGATTATCCTTTACTTAAAGAAGTTGAAGACCAATCAAAAGAAAATGATTATGTTAGATATAATATTTCAGTTTCTAAAGACAAAAAAGAAATATTGTCACGTAAAGATTTAGATAAAAAAATTGTAAAAGATGAAAATCCTAACTCAATTAATAGTCAATTAATGAATCGTAAAGTTAATGAATCATTTTCAGTAAATACACCAGAAAATGAAAACATTAAAATTACAGTTACTAAAGTTTTTCGTCCAATTAAAACTAAATTAACAAATGACAACATAAAAGAAATCAAAATTGAAGGCATTAATACTCTTGATGATCTTTATAATCAATTGTCAATGAATTTGAAAAATGAAATGGCTTCAAGTTATTTATTAGATTACATGAAAAAAGCAATTGAAACAGTTGGAAATAAATATGGTTTTGATTTACCACCTATTTTATTAGATACACAAGTAAATGATTTTATTAAAAGTAAATTAGCAATGTTACCTCCAGATAAAGCTAATCTATTAAGAAATGCAATAGAAAATAAACAAAAAGATGGTTATGAAATTTTTGATTTAGCAAAAACAAATGTTCGTAATTCTCTTTTAGACGCAATTATTGAATTTACTTTTTCATTGTTAATTAAAAACAAAATTACAGAAAAAGAAGCAGAAGAATTTTACAAATCAATTAAAGAAAAAACTTTAATTTCAACTGGCTCAAATGAATTAACAAAAGCTCAATCTGATGTTATTTTAGCTAAACAAAAAGCAGCTCTTAACTTGCTTTATCTTAATGATAAAGAAAGTTATGAAGCTGTTTCAAAAGATTTAGAATTAACTATTTAA", "aa": "MESIKEGKKISPDQLIEEAFKSDTNKIVESMGKELKEKYLNCLAFSPIVVVEDITKDKAKAVVTIVIYPKAEFDKIDINIKPDGKYEMVTKGAIDEIYKKFVDDYPLLKEVEDQSKENDYVRYNISVSKDKKEILSRKDLDKKIVKDENPNSINSQLMNRKVNESFSVNTPENENIKITVTKVFRPIKTKLTNDNIKEIKIEGINTLDDLYNQLSMNLKNEMASSYLLDYMKKAIETVGNKYGFDLPPILLDTQVNDFIKSKLAMLPPDKANLLRNAIENKQKDGYEIFDLAKTNVRNSLLDAIIEFTFSLLIKNKITEKEAEEFYKSIKEKTLISTGSNELTKAQSDVILAKQKAALNLLYLNDKESYEAVSKDLELTI", "aa_hexdigest": "33333a684d6b637092c16af47ea4ca91", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XEP6", "query_cov": 1.0, "subject_cov": 0.9223300970873787, "identity": 1.0, "score": 713.0, "evalue": 2.15e-258, "valid": true, "product": "trigger factor-related chaperone", "uniref50_id": "UniRef50_C4XEP6" }, "pscc": { "uniref50_id": "UniRef50_C4XEP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEP6" ], "product": "Trigger factor" }, "genes": [], "id": "OAIKOHHJFB_725", "locus": "GCA000209735_00701" }, { "type": "cds", "sequence": "contig_1", "start": 395784, "stop": 396122, "strand": "-", "frame": 3, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030841F7C", "UniRef:UniRef90_UPI0024C76683" ], "nt": "ATGAGAGGCTTCTTAGGAAGAATGTTATTTTCAAATGATTCAGTATTTAAAAATGTTAAAGTAACTTCAGGTGGAGAAAAAGCGAGATTGATGTTTTCAAGAATGATGCTTTTAGAATCTAACTTCTTGATTCTAGATCAACCTTTAGATCACCTTGATGCTGAAAGCATTGACAGTGTCATTGCAGGATTAAAAGCTTATAAGGGTGGTGTTATTGTAACAACATATAATAGAGCTTTAGTTAACCAAGTAGCTAATGTTATTGTTGAAATTGCTCCTGATAAAAGTTTTATTTATCATGGAACACTAGATGAATATGAAAAAATCATGAACTATTAA", "aa": "MRGFLGRMLFSNDSVFKNVKVTSGGEKARLMFSRMMLLESNFLILDQPLDHLDAESIDSVIAGLKAYKGGVIVTTYNRALVNQVANVIVEIAPDKSFIYHGTLDEYEKIMNY", "aa_hexdigest": "dce887d91e1c9a076258e30bafb102fa", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 395790, "stop": 396422, "observations": { "mutation": [ 396173, 396185 ], "stop": [ 396173, 396185 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0024C76683", "query_cov": 0.9910714285714286, "subject_cov": 0.4847161572052402, "identity": 0.8109999999999999, "score": 176.0, "evalue": 1.04e-53, "gene_start": 1, "gene_end": 110, "reference_start": 116, "reference_end": 226, "reference_sequence": "MNRDHGKQILNVENLTYKNENGDVLFQNISFSLKPGEKMVIVGEDDIAKTRLLECLYGIKKPTSGTIEWGQTITPSYFPNDNSKFFETDETILEWISKWPLENKEKVNQENDDARMRGFLGRMLFSNDSVFKKVNVTSGGEKFRLMFSRMMLLESNFLILDQPLDHLDTESIDSVIEGVNNYKGGAIFTTYNRAFVNQCANVILELQSPEKSFIFRGTLEEYEQIMLDE" }, "paralog": false, "identity": 0.6966824644549763, "score": 291.0, "evalue": 1.33e-101, "description": "Internal stop codon at 396173, 396185. Nonsense mutation around 396173, 396185.", "psc": { "uniref90_id": "UniRef90_UPI0024C76683", "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI0030841F7C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0024C76683", "UniRef:UniRef50_UPI0030841F7C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0030841F7C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030841F7C" ], "product": "ATP-binding cassette domain-containing protein" } }, "id": "OAIKOHHJFB_726", "locus": "GCA000209735_00702" }, { "type": "cds", "sequence": "contig_1", "start": 396183, "stop": 396425, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "TTGACTTATGTTGATGAAAAAGGAAATACACTATTCCAAAATGTTTCATTCACTTTAGCTCGTGGTGAAAAAATGGTTTTAATTGGTGAAGATGATATTGCTAAAACTAGATTTTTAGAATGCTTAATTGGTAAGATAAAACCAACAAAAGGCACTATAAAATGGGGCCAAACAATAAAACACACTTATTATCCAAATGAAAATAAAGAATATTTTAAAGAAGATATAACAATTCTAGAATGA", "aa": "MTYVDEKGNTLFQNVSFTLARGEKMVLIGEDDIAKTRFLECLIGKIKPTKGTIKWGQTIKHTYYPNENKEYFKEDITILE", "aa_hexdigest": "fb132871bd886214785d80abd84b6037", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 57, "aa_cov": 0.7, "hmm_cov": 0.9122807017543859, "evalue": 6.352413942737515e-09, "score": 32.64236068725586, "start": 13, "stop": 68 } ], "seq_stats": { "molecular_weight": 9305.617999999995, "isoelectric_point": 6.581352806091309 }, "id": "OAIKOHHJFB_727", "locus": "GCA000209735_00703" }, { "type": "cds", "sequence": "contig_1", "start": 396471, "stop": 396647, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00220019C3", "UniRef:UniRef90_UPI0021FF81A7", "PFAM:PF12848.13" ], "nt": "ATGAAACAATCAAATGCTAAAAAAGAAGTACAAATTGAAAAACTTAAAGCATTTATTGCTCGTTTTAGCGCCAACGCTTCTAAAAGTAAACAAGCAACCAGTAGAAAAAAATCTTTAGAAAAAATTACTCTTGATGAAATTAAACCTTCTAATCGTAAATATCCTTATATTAATTGA", "aa": "MKQSNAKKEVQIEKLKAFIARFSANASKSKQATSRKKSLEKITLDEIKPSNRKYPYIN", "aa_hexdigest": "bf4955c82b17bcc239a7c115be587dbd", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 396351, "stop": 396704, "observations": { "mutation": [ 396473, 396680 ], "stop": [ 396473, 396680 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0021FF81A7", "query_cov": 0.9827586206896551, "subject_cov": 0.4830508474576271, "identity": 0.877, "score": 94.0, "evalue": 1.36e-23, "gene_start": 1, "gene_end": 57, "reference_start": 20, "reference_end": 76, "reference_sequence": "MYTGNYSFWKQSSELARELMKQSNVKKEAQMEKLKEFIARFSANASKSKQYTSRKKALEKITLDEIKPSNRKYPFIRWDMNRDHGKQILNVENLTYKNENGDVLFQNISFSLKPGEKW" }, "paralog": false, "identity": 0.7606837606837606, "score": 176.0, "evalue": 1.49e-58, "description": "Internal stop codon at 396473, 396680. Nonsense mutation around 396473, 396680.", "psc": { "uniref90_id": "UniRef90_UPI0021FF81A7", "uniref50_id": "UniRef50_UPI00220019C3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0021FF81A7", "UniRef:UniRef50_UPI00220019C3" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00220019C3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00220019C3" ] } }, "hypothetical": true, "pfams": [ { "id": "PF12848.13", "name": "ABC_tran_Xtn", "length": 54, "aa_cov": 0.9310344827586207, "hmm_cov": 0.9629629629629629, "evalue": 3.5165151778769183e-14, "score": 48.78986740112305, "start": 3, "stop": 56 } ], "seq_stats": { "molecular_weight": 6685.7099, "isoelectric_point": 10.248297309875486 }, "id": "OAIKOHHJFB_728", "locus": "GCA000209735_00704" }, { "type": "cds", "sequence": "contig_1", "start": 396819, "stop": 396941, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGTCAGAATTAACTGCTAATCAAAAAATTAAAGTTTTATTAGCCAAAGCATTATTTGGTAATCCTGATATTTTAATCATGGACGAACCTACTAACAATCTTGACTTGCGTGCAATTAAATGA", "aa": "MSELTANQKIKVLLAKALFGNPDILIMDEPTNNLDLRAIK", "aa_hexdigest": "c3f1ca6a81b111cbf9e4bb5c858949f1", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 31, "aa_cov": 0.775, "hmm_cov": 1.0, "evalue": 1.2634304294220547e-06, "score": 25.197847366333008, "start": 2, "stop": 32 } ], "seq_stats": { "molecular_weight": 4453.2288, "isoelectric_point": 6.0008562088012685 }, "id": "OAIKOHHJFB_729", "locus": "GCA000209735_00705" }, { "type": "cds", "sequence": "contig_1", "start": 397011, "stop": 397397, "strand": "-", "frame": 3, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002614D0AA" ], "nt": "ATGATAGAAGTAAATAATCTAAGTAAAATTTTTAGCGACAAAAAATTATTTGAAAATGTTAATTTAAAATTTACAGCTGGAAATACATATGGAATAATTGGAGCCAATGGAGCTGGCAAAAGTACATTTTTAAAAATTTTAGCTGGTGAAATAGAAGCTACCGGCGGCCAAATAATAATTGAAAAAAGCCGAAGACTTAGTGTTTTAAGCCAAGATCATAATGCTTATGACAATATGAATGTAACCGATGTTGTAGTTATGGGAAATACTGATTTATATAAAATCAAACAAGAAAAAGATGCTATTTATGCAAATCCTGAAGCAACAATGGAAGACTATACAAGGGCAGGAGAATTAGAAGAAAAATTCGGCGAATTAGGTGGTTGA", "aa": "MIEVNNLSKIFSDKKLFENVNLKFTAGNTYGIIGANGAGKSTFLKILAGEIEATGGQIIIEKSRRLSVLSQDHNAYDNMNVTDVVVMGNTDLYKIKQEKDAIYANPEATMEDYTRAGELEEKFGELGG", "aa_hexdigest": "f387d22bab52c37a23d576cc4bd3c611", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI002614D0AA", "query_cov": 1.0, "subject_cov": 0.9343065693430657, "identity": 0.57, "score": 138.0, "evalue": 9.1e-40, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI002614D0AA" }, "pscc": { "uniref50_id": "UniRef50_UPI002614D0AA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002614D0AA" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_730", "locus": "GCA000209735_00706" }, { "type": "cds", "sequence": "contig_1", "start": 397480, "stop": 398070, "strand": "-", "frame": 2, "gene": null, "product": "trigger factor-related chaperone", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEP8", "UniRef:UniRef90_C4XEP8" ], "nt": "ATGCCACTAACCAATCAAAATATTTATTTTCTTAAAATACCTTATGTTACAAATCTAATTACTTTAAAAAACTTTTTCTTTTCAATGACTAAAAAACAAAATGCCATTAAAGAAGTTATGAGATATTTTGACAGCTTAATTAGTGAATTAGAAAATACTAATACAATTATTATTCCTGATGAATTTATAAATAGCAAAATATCAGATTATACTCATCAATATTTAGAAAAAGTAAATCCAAATAATCGAGCAGCTATTGAAAAAAGTATTCAAGAAAAAGATCAATATGGTAAACAAATAATAGATAATGCGATTAATAGTGCTAGAAGAGAAATTGTTGAAGACATTGTTAAAAAATTAGTTTACACATTAGAAAAAATAAAAGTCCATGACACAGAAGTAAATCAAGAATTAAATCTAAGCGAAAATATTTTATTTCAATCAAACAACTATTACAATAGAAATGAAAACAAAAATGTTAACACTGTAAAAGAAGATTTAATTAATATAAAAATAGCTTTAAAACTTTTACAACTAAATGCACCTCAATTATTTTTAGTAATTGCTAGAGATGCAGCTATTGCAGTATAG", "aa": "MPLTNQNIYFLKIPYVTNLITLKNFFFSMTKKQNAIKEVMRYFDSLISELENTNTIIIPDEFINSKISDYTHQYLEKVNPNNRAAIEKSIQEKDQYGKQIIDNAINSARREIVEDIVKKLVYTLEKIKVHDTEVNQELNLSENILFQSNNYYNRNENKNVNTVKEDLINIKIALKLLQLNAPQLFLVIARDAAIAV", "aa_hexdigest": "6337901f89b1b553956cb07860501614", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 397480, "stop": 398370, "observations": { "mutation": [ 398109 ], "stop": [ 398109 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEP8", "query_cov": 1.0, "subject_cov": 0.4666666666666667, "identity": 1.0, "score": 366.0, "evalue": 2.11e-124, "gene_start": 1, "gene_end": 196, "reference_start": 225, "reference_end": 420, "reference_sequence": "MRFFKWFGGRMINYLLKRENIKMNKDEIEAMINNTVRDLKIDRTKIDSMSLLEKSFDYKCKKIYNLISNEILKSDALAYIASPAIRKDIISNNTISAEIVVAYYPFEELKKINYKNTPVNYIYNFFDENSIGAIFENTIKNYTFKNSVNDFIKQNDLVKLNIKLFQNNILMKDDKNVLIIANHTNNFTINNSILNHRYGEIFSINDPLGRKWEMQVIEIKRTVSMPLTNQNIYFLKIPYVTNLITLKNFFFSMTKKQNAIKEVMRYFDSLISELENTNTIIIPDEFINSKISDYTHQYLEKVNPNNRAAIEKSIQEKDQYGKQIIDNAINSARREIVEDIVKKLVYTLEKIKVHDTEVNQELNLSENILFQSNNYYNRNENKNVNTVKEDLINIKIALKLLQLNAPQLFLVIARDAAIAV" }, "paralog": false, "identity": 0.9966216216216216, "score": 577.0, "evalue": 9.45e-210, "description": "Internal stop codon at 398109. Nonsense mutation around 398109.", "psc": { "uniref90_id": "UniRef90_C4XEP8", "product": "trigger factor-related chaperone", "uniref50_id": "UniRef50_C4XEP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEP8", "UniRef:UniRef50_C4XEP8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEP8" ], "product": "Trigger factor C-terminal domain-containing protein" } }, "id": "OAIKOHHJFB_731", "locus": "GCA000209735_00707" }, { "type": "cds", "sequence": "contig_1", "start": 398107, "stop": 398712, "strand": "-", "frame": 2, "gene": null, "product": "trigger factor-related chaperone", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEP8", "UniRef:UniRef90_C4XEP8" ], "nt": "ATGATCAATTATTTATTAAAAAGAGAAAATATTAAAATGAATAAAGATGAAATTGAGGCAATGATAAATAATACTGTTAGAGATTTAAAAATTGACAGAACTAAAATTGATTCAATGTCTTTATTAGAAAAATCTTTTGATTATAAATGCAAAAAAATATATAACTTAATTTCAAATGAAATTTTAAAAAGTGATGCTTTAGCCTATATTGCTAGTCCAGCAATTAGAAAAGATATTATTTCAAATAACACTATTAGTGCGGAAATTGTGGTTGCTTATTATCCTTTTGAAGAGCTTAAAAAAATTAACTATAAAAATACTCCAGTAAATTATATTTACAACTTTTTTGATGAAAATTCAATTGGTGCAATTTTTGAAAATACAATTAAAAATTACACATTTAAAAATTCAGTAAATGATTTTATTAAACAAAACGACTTAGTTAAGTTAAACATTAAATTATTTCAAAATAATATCTTAATGAAAGATGATAAAAATGTTCTTATAATAGCTAACCACACAAACAACTTTACTATTAATAATTCAATTCTTAATCATAGATATGGTGAAATTTTTAGCATCAATGATCCTTTAGGTAGAAAATGA", "aa": "MINYLLKRENIKMNKDEIEAMINNTVRDLKIDRTKIDSMSLLEKSFDYKCKKIYNLISNEILKSDALAYIASPAIRKDIISNNTISAEIVVAYYPFEELKKINYKNTPVNYIYNFFDENSIGAIFENTIKNYTFKNSVNDFIKQNDLVKLNIKLFQNNILMKDDKNVLIIANHTNNFTINNSILNHRYGEIFSINDPLGRK", "aa_hexdigest": "0a93f1f061d39da5c95b12f4aa85f321", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "both", "pseudogene": { "start": 397804, "stop": 398742, "observations": { "mutation": [ 398109, 398727 ], "stop": [ 398109, 398727 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEP8", "query_cov": 1.0, "subject_cov": 0.4785714285714286, "identity": 1.0, "score": 389.0, "evalue": 2.86e-133, "gene_start": 1, "gene_end": 201, "reference_start": 11, "reference_end": 211, "reference_sequence": "MRFFKWFGGRMINYLLKRENIKMNKDEIEAMINNTVRDLKIDRTKIDSMSLLEKSFDYKCKKIYNLISNEILKSDALAYIASPAIRKDIISNNTISAEIVVAYYPFEELKKINYKNTPVNYIYNFFDENSIGAIFENTIKNYTFKNSVNDFIKQNDLVKLNIKLFQNNILMKDDKNVLIIANHTNNFTINNSILNHRYGEIFSINDPLGRKWEMQVIEIKRTVSMPLTNQNIYFLKIPYVTNLITLKNFFFSMTKKQNAIKEVMRYFDSLISELENTNTIIIPDEFINSKISDYTHQYLEKVNPNNRAAIEKSIQEKDQYGKQIIDNAINSARREIVEDIVKKLVYTLEKIKVHDTEVNQELNLSENILFQSNNYYNRNENKNVNTVKEDLINIKIALKLLQLNAPQLFLVIARDAAIAV" }, "paralog": false, "identity": 0.9903846153846154, "score": 610.0, "evalue": 6.67e-223, "description": "Internal stop codon at 398109, 398727. Nonsense mutation around 398109, 398727.", "psc": { "uniref90_id": "UniRef90_C4XEP8", "product": "trigger factor-related chaperone", "uniref50_id": "UniRef50_C4XEP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEP8", "UniRef:UniRef50_C4XEP8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEP8" ], "product": "Trigger factor C-terminal domain-containing protein" } }, "id": "OAIKOHHJFB_732", "locus": "GCA000209735_00708" }, { "type": "tRNA", "sequence": "contig_1", "start": 399010, "stop": 399084, "strand": "+", "gene": "trnN", "product": "tRNA-Asn(gtt)", "amino_acid": "Asn", "anti_codon": "gtt", "score": 80.8, "nt": "GCCGATTTAGCTCAGCGGTAGAGCAGCTGGCTGTTAACCAGTTGGTCACAAGTTCAATCCTTGTAATCGGCGCCA", "db_xrefs": [ "SO:0000257" ], "anti_codon_pos": [ 399042, 399044 ], "id": "OAIKOHHJFB_5", "locus": "GCA000209735_00709" }, { "type": "tRNA", "sequence": "contig_1", "start": 399088, "stop": 399163, "strand": "+", "gene": "trnE", "product": "tRNA-Glu(ttc)", "amino_acid": "Glu", "anti_codon": "ttc", "score": 66.4, "nt": "GGTCTGTTGGTGAAGCGGTTAACACACATGGTTTTCATCCATGCATGCACGGGTTCGATTCCCGTACAGACTACCA", "db_xrefs": [ "SO:0000259" ], "anti_codon_pos": [ 399121, 399123 ], "id": "OAIKOHHJFB_6", "locus": "GCA000209735_00710" }, { "type": "tRNA", "sequence": "contig_1", "start": 399168, "stop": 399243, "strand": "+", "gene": "trnV", "product": "tRNA-Val(tac)", "amino_acid": "Val", "anti_codon": "tac", "score": 79.7, "nt": "GGAGAATTAGCTCAGTTGGGAGAGCATCGCCCTTACAAGGCGGAGGTCATGGGTTCGAGCCCCTTATTCTCCACCA", "db_xrefs": [ "SO:0000273" ], "anti_codon_pos": [ 399201, 399203 ], "id": "OAIKOHHJFB_7", "locus": "GCA000209735_00711" }, { "type": "tRNA", "sequence": "contig_1", "start": 399245, "stop": 399320, "strand": "+", "gene": "trnT", "product": "tRNA-Thr(tgt)", "amino_acid": "Thr", "anti_codon": "tgt", "score": 91.8, "nt": "GCCGTCTTAGCTCAGTTGGTAGAGCAACTGACTTGTAATCAGTAGGTCGTAGGTTCGAGTCCTATAGACGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 399278, 399280 ], "id": "OAIKOHHJFB_8", "locus": "GCA000209735_00712" }, { "type": "tRNA", "sequence": "contig_1", "start": 399412, "stop": 399496, "strand": "+", "gene": "trnL", "product": "tRNA-Leu(tag)", "amino_acid": "Leu", "anti_codon": "tag", "score": 59.4, "nt": "GCGCGAGTGGTGAAATTGGCAGACACGCTAGATTTAGGCTCTAGTGCTTTACGGCATGAGGGTTCAAGTCCCTCCCCGCGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 399440, 399442 ], "id": "OAIKOHHJFB_9", "locus": "GCA000209735_00713" }, { "type": "cds", "sequence": "contig_1", "start": 399658, "stop": 399795, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAAGATAATTAATTTTAAAATGTTTGAAAATGCAGCAATTAGACACAACTTGAAAATTTTGCAAGTTGATTATTTTGAAAAAAATATAACAATTGATATTTTTTGTGGCAACAATTCTTATATTAAATGA", "aa": "MKNKIINFKMFENAAIRHNLKILQVDYFEKNITIDIFCGNNSYIK", "aa_hexdigest": "babf2593f658da377b49c0a53648ce13", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5394.2961, "isoelectric_point": 9.302159309387207 }, "id": "OAIKOHHJFB_733", "locus": "GCA000209735_00714" }, { "type": "cds", "sequence": "contig_1", "start": 399944, "stop": 400108, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01330.27" ], "nt": "ATGGTTTTATATCGAATCGGAGAAATTGTTTATAAAAATAATCAAAATTTAATTTTTGAAAGTCAAGGAGTAGGTTATTCAATAATCTTTCCAGATCATGCTAGAGTTGAAGCTAAACAAAAAATGAAGTTGTATCTATATGAAATAAGAAACGATTACACTTGA", "aa": "MVLYRIGEIVYKNNQNLIFESQGVGYSIIFPDHARVEAKQKMKLYLYEIRNDYT", "aa_hexdigest": "48038776229e059bd268cfe29ed1aa7b", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01330.27", "name": "RuvA_N", "length": 51, "aa_cov": 0.9074074074074074, "hmm_cov": 1.0, "evalue": 7.747015408050398e-08, "score": 28.46644401550293, "start": 1, "stop": 49 } ], "seq_stats": { "molecular_weight": 6473.391900000002, "isoelectric_point": 8.005506706237792 }, "id": "OAIKOHHJFB_734", "locus": "GCA000209735_00715" }, { "type": "cds", "sequence": "contig_1", "start": 400409, "stop": 400546, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07499.19" ], "nt": "ATGTTAGGCTTTAAAGCAGCTCAAATTGATTCAGCATTAACTAAAGTTGAATTAAATCAAGATGTTGAATCAATGATTGAAGAAGCAATTAAATTTATTTCTCAAAGTGGTGCTCAAAATGAACAGCTTAATGCTTAG", "aa": "MLGFKAAQIDSALTKVELNQDVESMIEEAIKFISQSGAQNEQLNA", "aa_hexdigest": "7e7e499e2935b65702a950444eae9e99", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07499.19", "name": "RuvA_C", "length": 35, "aa_cov": 0.7333333333333333, "hmm_cov": 1.0, "evalue": 2.662620468409344e-08, "score": 30.333208084106445, "start": 2, "stop": 34 } ], "seq_stats": { "molecular_weight": 4911.4763, "isoelectric_point": 4.250954246520997 }, "id": "OAIKOHHJFB_735", "locus": "GCA000209735_00716" }, { "type": "cds", "sequence": "contig_1", "start": 400539, "stop": 401489, "strand": "+", "frame": 3, "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "db_xrefs": [ "EC:3.6.4.12", "GO:0003677", "GO:0005524", "GO:0006281", "GO:0006310", "GO:0009378", "SO:0001217", "UniRef:UniRef50_Q4A6N6", "UniRef:UniRef90_A0A3P8KA19" ], "nt": "ATGCTTAGACCAGCTAATTTTTTTGAATTTGTTGGCCAAGAAAAATTAATCTATACATTAAAGACAATGATCGAGGGTTCAAGGTATCGAAAGGAGCCTCTTGATCATATTCTTTTTTATGGTCCACCAGGGACAGGAAAAACTTCTTTAGCTAGTATTTTGGCTAATGAATTAGATACCAAAATACATTATTTACAAGGTGCTCTTTTAGAAAAGAAAAGTGATATTTTAACTGTTTTTGCTAATGTTAAAGAAAATGATATCATCTTTATAGATGAAATTCATAGCATGAATAAAAATGTTGAAGAAATTATTTATAATGCTATGGAAGATTACAAAATCGACATAATTATAGGGCCTGAGGGAAATAGTAAGGTTATGAGAATGAATCTTAAACCTTTTACTTTAATTGGCGCAACTACTAAAATTAACTTATTAACTCAGCCACTTAAAGATCGTTTTGGTTTTATCGCCAGACTTAGTCAATATAATGAAAAAGAAATAGCAAAAATATTAGAAAACAGTGAAATGGTGTTAAATATCAAAAGTGAAAGAGGAGTCATTCCTTTGATTGCAAAATATTCCAAAGGGACACCAAGAGTTGCTAATCATTTATTAAAAAGAATATTAGATTTTTCAATTCAAAATAAAGAAGATGAAATAACTAAAAAGACAACTTACTCAACATTTAAACATCTAGAATTATATGATCTTGGTTTAGGCCGAGAACACATTGAATATTTAAAAGTTTTAAACGAAACATTTGAAAATAAATTTGCTGCAATAGATTCAATTGCTGGTGTTTTAAATATTGATCGAGATATTTTAATTTATGAATTAGAACCACTCTTGCTTTATTTAAAATTAATTGCCAAAGGGCCAAGAGGACGAAAAATTACAACAAAAGGAATAGATTATTTGCTAAAAAATAAGTTAGATATCCATTATTAG", "aa": "MLRPANFFEFVGQEKLIYTLKTMIEGSRYRKEPLDHILFYGPPGTGKTSLASILANELDTKIHYLQGALLEKKSDILTVFANVKENDIIFIDEIHSMNKNVEEIIYNAMEDYKIDIIIGPEGNSKVMRMNLKPFTLIGATTKINLLTQPLKDRFGFIARLSQYNEKEIAKILENSEMVLNIKSERGVIPLIAKYSKGTPRVANHLLKRILDFSIQNKEDEITKKTTYSTFKHLELYDLGLGREHIEYLKVLNETFENKFAAIDSIAGVLNIDRDILIYELEPLLLYLKLIAKGPRGRKITTKGIDYLLKNKLDIHY", "aa_hexdigest": "338f66fcfd1b8885ba5e1fdd2cbede44", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3P8KA19", "query_cov": 1.0, "subject_cov": 0.9875, "identity": 1.0, "score": 623.0, "evalue": 2.4e-225, "valid": true, "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "ec_ids": [ "3.6.4.12" ], "uniref50_id": "UniRef50_Q4A6N6", "go_ids": [ "GO:0003677", "GO:0005524", "GO:0006281", "GO:0006310", "GO:0009378" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A6N6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6N6" ], "product": "Holliday junction branch migration complex subunit RuvB" }, "genes": [ "ruvB" ], "id": "OAIKOHHJFB_736", "locus": "GCA000209735_00717" }, { "type": "cds", "sequence": "contig_1", "start": 401692, "stop": 402153, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAATTGAATATGGTGGAGGTGTTGAAATCTTAGTTCAAGCACAAAAAAATGGCAAAAAAGTTAACGATAAATATGCCAAGAATATTGCTAACACTATGGATGACCGTTTAACTGGTGGTATTGCATTTAATGGAACTACTGTTAATGTTGAAGGTGATGGGATTTTAAAAATTACTCAAAATGGTAAATTAAGTTCAAAAGAAAAAGAAGAATTAGAAAAAATTGTTTCTTCAAAACCAAATTTAATTCTTACTGATGGCGATATGATTCCTTTATTTGTTAGAGGAAAATTTAATGAATCTAGAGAAAAAATAGATTATTCAAGAATTGAAGATTACATTCCACCTCTTAAGGAAAATGGAGCAAAATTTAAATTTATTCCACCAAGTGAAAAAGGTCGAAGTGGCTATTATGGTATAGATGTTTCTTTAAGAAATAACGCTGCCGCTGACGAATGA", "aa": "MSIEYGGGVEILVQAQKNGKKVNDKYAKNIANTMDDRLTGGIAFNGTTVNVEGDGILKITQNGKLSSKEKEELEKIVSSKPNLILTDGDMIPLFVRGKFNESREKIDYSRIEDYIPPLKENGAKFKFIPPSEKGRSGYYGIDVSLRNNAAADE", "aa_hexdigest": "4667b0b884826d93bf19f3a9421556b0", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16875.891599999984, "isoelectric_point": 6.404697227478027 }, "id": "OAIKOHHJFB_737", "locus": "GCA000209735_00718" }, { "type": "cds", "sequence": "contig_1", "start": 402415, "stop": 403056, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02355.23" ], "nt": "TTGAAAGGTACTCAATTTTTCATTTCAGGTAGATTTAATGAAGCTCAAGCAAAAAAAACAGCAGCTAACATTAATTTTGGTTCAGCAAACTACGATTTAAAAATTTTATCTTCTAACTATATTGAAGCAAGCAAAACCTCAAATTCATTTAGAAGTGCATTGTTAGCTGGAGCAGTTGTCTTTAGTATTATTGCAGTCTTTATGATTGTTAACTATGGATTACTTGGTGCTTTAAGTACAATATCAATTGCTTTATATATGTTCTTAACTTTATTATTGTTCACAGTTTTACGTGGAGAATATTCACCAGTAACAATTGCTGCTTTAATTATTGGTATTGGAATTAGTGTCGACTCAAATATTATTACCTTCGAACGGCTCAAAAATGAGGTTTATGCAGGTGATAGGCTGAAGAAGGCTATGAAGAATGCAAATCATAGGACATTAGGTTCAATTTTAGATGCAAATATCTTAACTTTATTGATTTCATTTGTTCTATTCTTCTTTGGTACTTCAACTATTAAAGGCTTCAGTATTTCACTTGTATTCTCAGTTACATTCATCTTAATTGTTATGCTTATATTTACTAGAGGTTTAAGTGTATTGCTAGTAAATACAGGCGCATTCGACAAAAAACTGTGA", "aa": "MKGTQFFISGRFNEAQAKKTAANINFGSANYDLKILSSNYIEASKTSNSFRSALLAGAVVFSIIAVFMIVNYGLLGALSTISIALYMFLTLLLFTVLRGEYSPVTIAALIIGIGISVDSNIITFERLKNEVYAGDRLKKAMKNANHRTLGSILDANILTLLISFVLFFFGTSTIKGFSISLVFSVTFILIVMLIFTRGLSVLLVNTGAFDKKL", "aa_hexdigest": "2a71420e1fa4ac53e07a7acd7d525e25", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02355.23", "name": "SecD_SecF_C", "length": 161, "aa_cov": 0.7417840375586855, "hmm_cov": 1.0, "evalue": 4.371316146374248e-15, "score": 51.66889190673828, "start": 30, "stop": 187 } ], "seq_stats": { "molecular_weight": 23212.17610000001, "isoelectric_point": 9.897847175598145 }, "id": "OAIKOHHJFB_738", "locus": "GCA000209735_00719" }, { "type": "cds", "sequence": "contig_1", "start": 403243, "stop": 403353, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATTTGGTGGCGGAGTTAGAATTTCTATTGAAAGTGCTAATAGTGCTTATGTTAATAGTGATATTTTGGACATTCAAAACAAATTTATGGCTCATATGAATAAATGA", "aa": "MEFGGGVRISIESANSAYVNSDILDIQNKFMAHMNK", "aa_hexdigest": "bc270f5c0f2dd1d01024fd82a607eb59", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3988.4829, "isoelectric_point": 5.4648645401000975 }, "id": "OAIKOHHJFB_739", "locus": "GCA000209735_00720" }, { "type": "cds", "sequence": "contig_1", "start": 403393, "stop": 403629, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTAGGCAATGGGAACTTCAAACTTCAAATTGAAACTTCTCAAGACTTAAGTAATGTTTTAAGTAAAATTGAAAGTGATGTTGCAACATTCGTTTCTTCAGGAAACAAACCTAGATTTATAGCATATGCAATTTCAACTTCGCGTGCTTTTGACTTACTTAAATACTCAATGATTGCTATAGCTGGAGCATTCCTTGTTGTTATTGTATATACCTTTATTAGATTTAAATGA", "aa": "MDLGNGNFKLQIETSQDLSNVLSKIESDVATFVSSGNKPRFIAYAISTSRAFDLLKYSMIAIAGAFLVVIVYTFIRFK", "aa_hexdigest": "0ca290aeffc772b4f154dd0e124788c8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8643.935499999996, "isoelectric_point": 9.156267356872558 }, "id": "OAIKOHHJFB_740", "locus": "GCA000209735_00721" }, { "type": "cds", "sequence": "contig_1", "start": 403675, "stop": 404028, "strand": "+", "frame": 1, "gene": null, "product": "Protein export membrane protein SecD/SecF C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432F9G7" ], "nt": "ATGTCAATAGCATTTATTGTTGTAGCTAGATTGCAACTTTCACCAATTATGATTGCTGCATTATTATCAATTACAGCCTTTTCAATAAACGATACTATTATTGTTTTTGAAAGAATTAAAGAAACAATTAATACTCAATATCATGGTGTTTTCTTATCAAAAGCATTAATTAAAGAAATTGCAAACAACGCAATTAAAGATACATTCAAACGTAGTTTATACACAACATTAACCACTGTAGTATCAATTCTTGTTTTATTAATGTTTGGTAATGCTACTGATTTATCATTCAATATTGTTATGATATTTGGACTTGTTGTTGGTTCATATAGCTCAATCTTTATTTGTACATGA", "aa": "MSIAFIVVARLQLSPIMIAALLSITAFSINDTIIVFERIKETINTQYHGVFLSKALIKEIANNAIKDTFKRSLYTTLTTVVSILVLLMFGNATDLSFNIVMIFGLVVGSYSSIFICT", "aa_hexdigest": "b62689f9cb1c4fc95ff0771e77b94d8f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A432F9G7", "query_cov": 0.9743589743589743, "subject_cov": 0.9193548387096774, "identity": 0.579, "score": 109.0, "evalue": 9.97e-29, "valid": false, "product": "Protein export membrane protein SecD/SecF C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A432F9G7" }, "pscc": { "uniref50_id": "UniRef50_A0A432F9G7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432F9G7" ], "product": "Protein export membrane protein SecD/SecF C-terminal domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_741", "locus": "GCA000209735_00722" }, { "type": "cds", "sequence": "contig_1", "start": 404256, "stop": 404561, "strand": "+", "frame": 3, "gene": "truB", "product": "TruB family pseudouridylate synthase (N terminal domain)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF45FC1", "UniRef:UniRef90_UPI000FF45FC1" ], "nt": "ATGTTTTATTTAATTAAAAAAACAAAAGGAATTTCATCATTTTCTTGTATAAAAACATTTGCTAAAAAAATGAATATTAAAAAAATAGGACACAGCGGAACACTTGATCCCTTAGCCTCTGGCTTATTGTTATGTGCTAGTGAAGATGACACAAAATTAATACCTTATATTAGCCATAAAAGTAAAACATATATCAGTAAAATAATTTTTGGTAAACATACTTCTACATATGATTCTGAAGGTGAAATTACTAACACTTCAGAAGTCAAAATTAAGGAAGAAGATTTAGAAAAAATCAACAAATGA", "aa": "MFYLIKKTKGISSFSCIKTFAKKMNIKKIGHSGTLDPLASGLLLCASEDDTKLIPYISHKSKTYISKIIFGKHTSTYDSEGEITNTSEVKIKEEDLEKINK", "aa_hexdigest": "22f91c7a57129b1d5b2754c594a64c1a", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI000FF45FC1", "query_cov": 1.0, "subject_cov": 0.8859649122807017, "identity": 1.0, "score": 197.0, "evalue": 5.24e-64, "valid": true, "product": "TruB family pseudouridylate synthase (N terminal domain)", "uniref50_id": "UniRef50_UPI000FF45FC1" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF45FC1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF45FC1" ] }, "genes": [ "truB" ], "id": "OAIKOHHJFB_742", "locus": "GCA000209735_00723" }, { "type": "cds", "sequence": "contig_1", "start": 404814, "stop": 405107, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGAATTAGAAAGAATTAAAATAGGCAAACTAGCTTTAAGTAATACCAAAAATGAATATGAGCAAATTGAAATAAATAACTTATTTGATCTTGACTTTTATAATGCAACAGATAGTGAAATTGAATCTCTTAAAAAAGGTCAAGTTATTAACGCTAACAAAAAATTAAAAGACAATAAATATTTATTAATTAATAAAACAAAAGATCAAAACCTAATATTAGGAATTTTAGAATTAAAAAATAACAATTTAAAAGTTGTTAAATTATTTGGCAACCGATTAGAGGAGTAA", "aa": "MSELERIKIGKLALSNTKNEYEQIEINNLFDLDFYNATDSEIESLKKGQVINANKKLKDNKYLLINKTKDQNLILGILELKNNNLKVVKLFGNRLEE", "aa_hexdigest": "6f7e1c7ec8dc8dd5f9e3cb8b3a390db3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11254.82969999999, "isoelectric_point": 7.867995643615723 }, "id": "OAIKOHHJFB_743", "locus": "GCA000209735_00724" }, { "type": "cds", "sequence": "contig_1", "start": 405110, "stop": 405478, "strand": "+", "frame": 2, "gene": null, "product": "YcsE-related riboflavin metabolism phosphatase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B5A7", "UniRef:UniRef90_A0A3P8LIG5" ], "nt": "ATGAAAAAAGAAGAATTAAAAGAAAATATTAAATTAGCTGCTTTCGATATTGATGGCACAATTTTACCTTATGCTCAACCAGAATTCAGCAATAATATTGAATTAATGTTTAAAAAACTAGAAGAATCAAACATTAAATCAGCTCTAGCCACAGCTAGGGAATTTGTTACTATTGGTAAATTAATGAACAAAGCTTCTAACCTTGATTATTTTATTGGCGCTAATGGTGCTTTTGTTTATGACTGCAAACACAAAGATTTAATTTTTGAACGCACAATTAAATTTGAAGACTTCAAAATTCTTTATGAATTCTTTAACAAATATATTACCTGTGAAGGATTCACGATAATGGATCGTAATTATGGTTGA", "aa": "MKKEELKENIKLAAFDIDGTILPYAQPEFSNNIELMFKKLEESNIKSALATAREFVTIGKLMNKASNLDYFIGANGAFVYDCKHKDLIFERTIKFEDFKILYEFFNKYITCEGFTIMDRNYG", "aa_hexdigest": "f68b02b222dbfe34258929425b1c9a06", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 405080, "stop": 405781, "observations": { "mutation": [ 405476, 405506, 405683, 405740 ], "stop": [ 405476, 405506, 405683, 405740 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8LIG5", "query_cov": 1.0, "subject_cov": 0.4206896551724138, "identity": 1.0, "score": 245.0, "evalue": 5.03e-80, "gene_start": 1, "gene_end": 122, "reference_start": 11, "reference_end": 132, "reference_sequence": "MIWQPIRGVIMKKEELKENIKLAAFDIDGTILPYAQPEFSNNIELMFKKLEESNIKSALATAREFVTIGKLMNKASNLDYFIGANGAFVYDCKHKDLIFERTIKFEDFKILYEFFNKYITCEGFTIMDRNYGWYSPKMNINTWFLSPHQAKMKPVDYDAVEKDHLHVITITAKDIKSTNDCAKKAKELIEKYNMDLEVNSQWATGVFVSPKGITKSHTLDWLAKYLKLDSKKNLIAFGDSSNDYEMIRDAAYGVTMEKASEYLKAVANDVALDPTSDGAYFKLKELDLIK" }, "paralog": false, "identity": 0.9785407725321889, "score": 455.0, "evalue": 5.89e-165, "description": "Internal stop codon at 405476, 405506, 405683, 405740. Nonsense mutation around 405476, 405506, 405683, 405740.", "psc": { "uniref90_id": "UniRef90_A0A3P8LIG5", "product": "YcsE-related riboflavin metabolism phosphatase", "uniref50_id": "UniRef50_A0A449B5A7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8LIG5", "UniRef:UniRef50_A0A449B5A7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449B5A7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B5A7" ], "product": "COF family HAD hydrolase protein" } }, "id": "OAIKOHHJFB_744", "locus": "GCA000209735_00725" }, { "type": "cds", "sequence": "contig_1", "start": 405533, "stop": 405685, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACCAGTTGATTATGATGCTGTGGAAAAAGACCACCTTCATGTCATAACTATTACAGCTAAAGACATTAAAAGCACAAATGATTGTGCTAAAAAAGCAAAAGAATTAATTGAAAAATACAACATGGATTTAGAAGTTAATTCACAATGA", "aa": "MKPVDYDAVEKDHLHVITITAKDIKSTNDCAKKAKELIEKYNMDLEVNSQ", "aa_hexdigest": "19fc09a76ae14d87ebb826c9a48e764f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5734.4913, "isoelectric_point": 5.507266426086424 }, "id": "OAIKOHHJFB_745", "locus": "GCA000209735_00726" }, { "type": "cds", "sequence": "contig_1", "start": 405815, "stop": 405952, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTCGAGATGCAGCTTATGGTGTTACTATGGAAAAAGCAAGTGAATACTTAAAAGCTGTGGCTAATGATGTAGCTCTTGATCCAACATCTGATGGAGCTTACTTTAAACTAAAAGAATTAGACTTAATTAAATAA", "aa": "MIRDAAYGVTMEKASEYLKAVANDVALDPTSDGAYFKLKELDLIK", "aa_hexdigest": "feb24920720995d9d47f54d63584c650", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4965.651199999998, "isoelectric_point": 4.716181373596191 }, "id": "OAIKOHHJFB_746", "locus": "GCA000209735_00727" }, { "type": "cds", "sequence": "contig_1", "start": 405972, "stop": 406844, "strand": "+", "frame": 3, "gene": null, "product": "FAD synthase", "db_xrefs": [ "RefSeq:WP_013354548.1", "SO:0001217", "UniParc:UPI0001E330A9", "UniRef:UniRef100_C4XEQ5", "UniRef:UniRef50_C4XEQ5", "UniRef:UniRef90_C4XEQ5" ], "nt": "ATGTCTTTAAAAGTTTATACATTTGATAATATTCCTAAATTTCAAAAACCTATTTTCATAATAGGCTCTTTTGAATCATTCCATTTAGGTCATTATAAACTTTTAGAAAAAGCTCGTGAATTAGCAAAAGAAAGTGAAAAAGAAAGAGACATTGTTTTAGTTTATTTTGAAGATATTGAGAATTTATCTAAAACAAAAAACTTAATTTTTTCAGATGCAAAAAACCGCGTTCAAGAGTTTGCAAATTTAAAAATAAATTTTGCTATAAGCCTGTTTTATAGTCAAATTAGTCATTTAAGTGCCCAAGATTTTATTGATAAATTAATAGCAAATCAAGATGATTATTCTTTTGTAATTGGTGAAGAATTTAAATTTGGCTTTAAACGTTCAGGCAATGTTCAATTCTTAGAAAATCATTACAATAAAGATAAAGTTTTTGCTCAAGAAGTTGTGAGATTAAAAAACAAACAAAAGATTTCAACTTCATATTTAAAAGAATTAATTGAATTTGGAGACATTGATTTACTTAACACTATCAACATGTTTCCTTATAGTTTTAGTGCTAAATTTTCAACTATTGAAAATAAAATTGAATTAAAAAAACATGAACTTTTAATTCCGCTAAGACAAGGTATTTATGCAGTTTTAGTTGAATTAAATAATTACAATTATTATGCATTAATGCAAGTTAATTTTGACACTAAATATTTCATTGAATTCTTAGATTTTAAAATCAAAGAAAACCAAAACATTGATGCTCGAATTAAAGTTTTAAAAGCGATTAGATTCTTTAGAAATAAAGAACTAGAATCAATTAATGAACAAAACAAATTAAATGCCAAAAGCATACTTTTAGATCTTAATAATAATTAG", "aa": "MSLKVYTFDNIPKFQKPIFIIGSFESFHLGHYKLLEKARELAKESEKERDIVLVYFEDIENLSKTKNLIFSDAKNRVQEFANLKINFAISLFYSQISHLSAQDFIDKLIANQDDYSFVIGEEFKFGFKRSGNVQFLENHYNKDKVFAQEVVRLKNKQKISTSYLKELIEFGDIDLLNTINMFPYSFSAKFSTIENKIELKKHELLIPLRQGIYAVLVELNNYNYYALMQVNFDTKYFIEFLDFKIKENQNIDARIKVLKAIRFFRNKELESINEQNKLNAKSILLDLNNN", "aa_hexdigest": "8be05d7590d4d50ccd4ba131fd99ec20", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E330A9", "ncbi_nrp_id": "WP_013354548.1", "uniref100_id": "UniRef100_C4XEQ5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330A9", "RefSeq:WP_013354548.1", "UniRef:UniRef100_C4XEQ5" ] }, "ips": { "uniref100_id": "UniRef100_C4XEQ5", "uniref90_id": "UniRef90_C4XEQ5" }, "psc": { "uniref90_id": "UniRef90_C4XEQ5", "product": "FAD synthase", "uniref50_id": "UniRef50_C4XEQ5" }, "pscc": { "uniref50_id": "UniRef50_C4XEQ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEQ5" ], "product": "FAD synthase" }, "genes": [], "id": "OAIKOHHJFB_747", "locus": "GCA000209735_00728" }, { "type": "cds", "sequence": "contig_1", "start": 407059, "stop": 407649, "strand": "+", "frame": 1, "gene": null, "product": "nucleotidyltransferase", "db_xrefs": [ "EC:6.3.1.22", "KEGG:K27343", "SO:0001217", "UniRef:UniRef50_C4XEQ6", "UniRef:UniRef90_C4XEQ6" ], "nt": "ATGAGTGATAAATTTAGCCAACGTGGAGAATTAACAGTTGTTCCTTTTAGTATTAGAAAAAAATATGCAAAAAAATATGGTGTCAATAAAGTAATTAAATTGAAATTTGAAGAAACTGTGCAAGCAGCTCATATTTTTGCATACAATGCTGTAATGAAACTTTACAAAGCTAAAGTTGACAAAATTGTTTTTGGTAGTGAAAGTAACAATCCAGATTCAATGCTTTATTGCGCTAAAGTTATGAAAGAAAAACATAATGAATTTTCTTTTGCTTTATTGCAAAAAATGAAAAAATCAGGTTTAAGTTATCCTAAAGCAGTTAGTGAAGTTATGAAAGAATTAGTTGGCGAAAGTTTTGAAATGCCAAATGATATCTTAGGTTTTGAATACATAAAAGTTATAGTATATAATAATTTGCCAATTAAGATTTATACATTAAGAAGAGAAGTTGGTTATCATTCGGATAAAGTTGTAGATGAATTTGCCTCAGCTTCATATTTAAGAAAACTTATTTATCAAGGTCAAGATATTAGAGCTTATAGTCCAATGACTTTTAAAAAAGTACCAAGAACTACAGCTTCACTATATTGA", "aa": "MSDKFSQRGELTVVPFSIRKKYAKKYGVNKVIKLKFEETVQAAHIFAYNAVMKLYKAKVDKIVFGSESNNPDSMLYCAKVMKEKHNEFSFALLQKMKKSGLSYPKAVSEVMKELVGESFEMPNDILGFEYIKVIVYNNLPIKIYTLRREVGYHSDKVVDEFASASYLRKLIYQGQDIRAYSPMTFKKVPRTTASLY", "aa_hexdigest": "03e392d826bce26bb90ca861f94cc335", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 406828, "stop": 407838, "observations": { "mutation": [ 407017, 407647 ], "stop": [ 407017, 407647 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEQ6", "query_cov": 1.0, "subject_cov": 0.5833333333333334, "identity": 1.0, "score": 384.0, "evalue": 1.29e-132, "gene_start": 1, "gene_end": 196, "reference_start": 78, "reference_end": 273, "reference_sequence": "MLIIISKKTNYFYFALLFASKINIWFFIFIYYNNKYLFKRRTMAVGIIAEYNPFHNGHIKQINWVKEHFPGEKIVVVMSDKFSQRGELTVVPFSIRKKYAKKYGVNKVIKLKFEETVQAAHIFAYNAVMKLYKAKVDKIVFGSESNNPDSMLYCAKVMKEKHNEFSFALLQKMKKSGLSYPKAVSEVMKELVGESFEMPNDILGFEYIKVIVYNNLPIKIYTLRREVGYHSDKVVDEFASASYLRKLIYQGQDIRAYSPMTFKKVPRTTASLYWKFQKIMNHTSLKKIKRIPLISEGIENLLKKNITYPTYESFVKHVLQKDIQLKNPKNNSLNFK" }, "paralog": false, "identity": 0.9910714285714286, "score": 661.0, "evalue": 1.37e-244, "description": "Internal stop codon at 407017, 407647. Nonsense mutation around 407017, 407647.", "psc": { "uniref90_id": "UniRef90_C4XEQ6", "product": "nucleotidyltransferase", "ec_ids": [ "6.3.1.22" ], "uniref50_id": "UniRef50_C4XEQ6", "kegg_orthology_id": "K27343", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEQ6", "EC:6.3.1.22", "UniRef:UniRef50_C4XEQ6", "KEGG:K27343" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEQ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEQ6" ], "product": "tRNA(Met) cytidine acetate ligase" } }, "id": "OAIKOHHJFB_748", "locus": "GCA000209735_00729" }, { "type": "cds", "sequence": "contig_1", "start": 407665, "stop": 407838, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCATACTTCACTTAAGAAAATCAAAAGAATACCTTTAATTTCAGAAGGTATTGAAAACTTACTTAAGAAAAATATTACTTATCCAACTTATGAATCATTTGTGAAGCATGTACTTCAAAAAGATATACAGCTCAAGAATCCAAAGAATAATAGCTTGAATTTTAAATAG", "aa": "MNHTSLKKIKRIPLISEGIENLLKKNITYPTYESFVKHVLQKDIQLKNPKNNSLNFK", "aa_hexdigest": "fe7f9a9dfc638b362e5513358b3ed3fe", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6708.8272000000015, "isoelectric_point": 9.998740196228027 }, "id": "OAIKOHHJFB_749", "locus": "GCA000209735_00730" }, { "type": "cds", "sequence": "contig_1", "start": 408018, "stop": 408425, "strand": "+", "frame": 3, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CA0BF5C" ], "nt": "ATGGACTCAACAAATAAGAAAAAAATCCTTGAAGTCCAAGGATTAAAAAAATATTTTTTTAACAACAACAAAATCAATAAAGCAGTTGATAATGTTAGCTTCAATTTATATGAAGGCCAAATTTTAGGTTTAATTGGTGAAAGTGGAAGTGGTAAAACCACTGTTGGAAGATCAATAGCAAAACTATATGATGATTTTCAAGGCCTTTTATTAGTTGATGGTAAATTAATTAACAATAAAAAAATGAGTGAAAGTGATCGTAAATTTTTAAGAAAAAATATTCAAATGATTTTTCAAAATCCTAAAAACTCATTGGATGAACAAAAAAGTATTTATTCAATTTTAAAAGAATCGTTGATAGCTAATAATATTTTAAAAATCAAAACAAAAGAAATCTTTAAAAATTGA", "aa": "MDSTNKKKILEVQGLKKYFFNNNKINKAVDNVSFNLYEGQILGLIGESGSGKTTVGRSIAKLYDDFQGLLLVDGKLINNKKMSESDRKFLRKNIQMIFQNPKNSLDEQKSIYSILKESLIANNILKIKTKEIFKN", "aa_hexdigest": "5e0d2e4b6d055a4df5b2ba9abe5ff329", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI00249F2F44", "query_cov": 0.9185185185185185, "subject_cov": 0.96875, "identity": 0.621, "score": 147.0, "evalue": 4.09e-43, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI000CA0BF5C" }, "pscc": { "uniref50_id": "UniRef50_UPI000CA0BF5C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CA0BF5C" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_750", "locus": "GCA000209735_00731" }, { "type": "cds", "sequence": "contig_1", "start": 408675, "stop": 408872, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGAATTCTATGAACAAAAACAAATTGACTATACTAATGGTAATTTTCCTTTTGGCATATTTGACTTTATTGAATTAGAAAACGAAAGAAAAAAAGCTTTAATTTTAACTAAAATGTCAAAAGAAAATTATGAATCAATGCAATTAATTGATAACTTAAATAATGAAATGAAAAACATTAAAAAAGAGTTTTGA", "aa": "MLEFYEQKQIDYTNGNFPFGIFDFIELENERKKALILTKMSKENYESMQLIDNLNNEMKNIKKEF", "aa_hexdigest": "d14b506dad54c79ae4b3d9f26d882cc5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7917.971699999996, "isoelectric_point": 4.8526517868041985 }, "id": "OAIKOHHJFB_751", "locus": "GCA000209735_00732" }, { "type": "cds", "sequence": "contig_1", "start": 408954, "stop": 409565, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATACTTTAGATGAAGAATTATACAAATTTGACTATAAAAAAGCTTTTTTAGCAAAAATGCAAATTGATTTATTAAATAAAAATAAGAAGCTAATTAACAACTTATTAGTTAAAGATATTAAAGAACTTATTTATGATTTAAAAAACTATAATTTAGATTTTTTTAATAAGTATTTCCAACCACTTGAATTTAACAAAAAAATCAAAGAAAATATGCAATCAACTTATGAAACATATTACGAATTTGATTTCAATAAATACATAATCAGAAGTAATTTGAATTTAACTCAATATGAAGAAAAAATTGACCACATTAAAAAATTAATTTCTAAGCAAATGATTCTATCTAAAAATGCCGGTGATGTTAAAAATTCAATTGAATATTTAAATGAGTTGAAAACAAAAATAGAAAAAATTGATCTTGAATTTGAAGATAAACTTGTAAATCATAAAGAAGAAACACTTAAAAAAGTGAAACAAGTTGAAACTGAATTAGATGAAAAAGAAAAAATTAATAATGTTTTAAAAATCAGGCAATCTAATTTTTATCAAAATTATTCAGACATCAAATTACTTAAACTTTTCAATACTTTTTTAGCTAATTGA", "aa": "MNNTLDEELYKFDYKKAFLAKMQIDLLNKNKKLINNLLVKDIKELIYDLKNYNLDFFNKYFQPLEFNKKIKENMQSTYETYYEFDFNKYIIRSNLNLTQYEEKIDHIKKLISKQMILSKNAGDVKNSIEYLNELKTKIEKIDLEFEDKLVNHKEETLKKVKQVETELDEKEKINNVLKIRQSNFYQNYSDIKLLKLFNTFLAN", "aa_hexdigest": "67a7453ca611bcae005df33fb02c1ac2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24647.12770000004, "isoelectric_point": 8.653800773620606 }, "id": "OAIKOHHJFB_752", "locus": "GCA000209735_00733" }, { "type": "cds", "sequence": "contig_1", "start": 409716, "stop": 410348, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4R0XTG3" ], "nt": "ATGGGATTAAGTCATAAAAACTCAAAAAGTTTTTTTGGAGTTTTCAAAGACTTTTTTACTAATCAAAAAATAAAAAGAAATGTTAAAGAATTATTATGCAAAAACATAATTTATAAAGCTTTAGAAGATGTCGGATTACTTAAACAATTTGCTTACCGTTATCCACATGAATTCTCAGGTGGTCAATTACAAAGAGTTGCAATAGCTAGAGCCTTAATTGTTGAACCTAAAATAATTATTGCTGATGAACCTATTGCTTCACTAGACATTTCAATTCAAGCACAAGTAGTTAACTTGCTTAAAGAACTATGCGAAAAAAGAAACATTGGAGTTATCTTTATAGCTCATGATCTTTCGATGATTGAATATTTAGCTGATGAAGTGCAAATTATGCATCATGGTAAATTTGTTGAAGGCGGTAAAACCAATATTATCTACAAAAAACCAATGCACCCTTATACTCAAAACTTATTTGCCTCAATTCCTAGAGTTGAAAATGCTAATAAAAAATTTATCAACCAAGACTTTGAAGCTAAATATCTCGAAGAACAAAAATTTTCTAATACAACTGATTTATACCTAATTGAGCCAAATCACTATTTATATGGAACTAGAAATCAAGTGCTCAAGTGA", "aa": "MGLSHKNSKSFFGVFKDFFTNQKIKRNVKELLCKNIIYKALEDVGLLKQFAYRYPHEFSGGQLQRVAIARALIVEPKIIIADEPIASLDISIQAQVVNLLKELCEKRNIGVIFIAHDLSMIEYLADEVQIMHHGKFVEGGKTNIIYKKPMHPYTQNLFASIPRVENANKKFINQDFEAKYLEEQKFSNTTDLYLIEPNHYLYGTRNQVLK", "aa_hexdigest": "10e973adcf75266caea3e3530c76d264", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0024CC3F60", "query_cov": 0.9333333333333333, "subject_cov": 0.9468599033816425, "identity": 0.633, "score": 246.0, "evalue": 7.85e-80, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A4R0XTG3" }, "pscc": { "uniref50_id": "UniRef50_A0A4R0XTG3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4R0XTG3" ], "product": "ABC transporter domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_753", "locus": "GCA000209735_00734" }, { "type": "cds", "sequence": "contig_1", "start": 410798, "stop": 410995, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTTAGATACAATTAGTTCACTTGCTTTATTAACTCCAGTTTTGGCTGCTAGTTGTAAGCCAATTTTAGTTGTTAAAGAAGAATATAACAAGGAAAAAAGTTATCTTAAAACATTTCTAAAAACTATTAATAGCAAAAGCGATCTAGAAAAAAATGAATTCAAAAAATTTAGAATTAATTTTCATACTTTTTAA", "aa": "MILDTISSLALLTPVLAASCKPILVVKEEYNKEKSYLKTFLKTINSKSDLEKNEFKKFRINFHTF", "aa_hexdigest": "106acd6d13e317bcbc94095e529e3111", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7538.819599999997, "isoelectric_point": 9.374750709533693 }, "id": "OAIKOHHJFB_754", "locus": "GCA000209735_00735" }, { "type": "cds", "sequence": "contig_1", "start": 411197, "stop": 411493, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FEECEC7", "UniRef:UniRef90_UPI000FEECEC7" ], "nt": "TTGCCATTAAGCACTATAATTTCCACATTTGCTAAGAATTTTTTAAATAAAAAAGTTAATATAAAGCAAGACATTTTAGAATCTTTTTATTTTAAAAACAACAAAAATAGAAAAATCTTTGTTTCATTAACAATCGTTTCAATGTTAATTACCTTTTTATTGGTTTTTTTACCAATTTTTGTTAGTTTAAATAATTATATTTATTTGCCTTTAATAATTGTTTTTTTAAGTTGTTCAACTATTTTTGCTTTTGTTTATTACGCTTTTTCTTATGCTACAGTTCGATTACAAGAATGA", "aa": "MPLSTIISTFAKNFLNKKVNIKQDILESFYFKNNKNRKIFVSLTIVSMLITFLLVFLPIFVSLNNYIYLPLIIVFLSCSTIFAFVYYAFSYATVRLQE", "aa_hexdigest": "c9c5ba0b2045cbcfddbd7c7a8d9c4e8b", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 411110, "stop": 411625, "observations": { "mutation": [ 411119, 411491 ], "stop": [ 411119, 411491 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000FEECEC7", "query_cov": 1.0, "subject_cov": 0.5664739884393064, "identity": 0.99, "score": 176.0, "evalue": 7.38e-55, "gene_start": 1, "gene_end": 98, "reference_start": 30, "reference_end": 127, "reference_sequence": "MPIWGQILLVLFSSIFLSTIFVVLTYKFFLPLSTIISTFAKNFLNKKVNIKQDILESFYFKNNKNRKIFVSLTIVSMLITFLLVFLPIFVSLNNYIYLPLIIVFLSCSTIFAFVYYAFSYATVRLQEWIESNKGYEEEFTFYNKAYEYQIDFFDFDLKSAKYMVVESNNLLRL" }, "paralog": false, "identity": 0.9824561403508771, "score": 325.0, "evalue": 4.91e-116, "description": "Internal stop codon at 411119, 411491. Nonsense mutation around 411119, 411491.", "psc": { "uniref90_id": "UniRef90_UPI000FEECEC7", "uniref50_id": "UniRef50_UPI000FEECEC7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000FEECEC7", "UniRef:UniRef50_UPI000FEECEC7" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000FEECEC7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FEECEC7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11468.611399999989, "isoelectric_point": 9.673819541931152 }, "id": "OAIKOHHJFB_755", "locus": "GCA000209735_00736" }, { "type": "cds", "sequence": "contig_1", "start": 411596, "stop": 411850, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGTCGAATCAAATAATTTATTGCCGGCTTTAAAAGTTAAACATTTTATTAGCTTTAAAAAGTTACAAAAAATATACAAAAAAACACTTCATATTCAACTTTTAGAAAATGAATGTTACCTTTATTCATTGATTAATACTGATCATTGTTTTATTAAAAAAACCAAGGTCAAAAATATACAATATTTTGCCCTATTGCAAGATTTTATAGAGAAATTTAAAAAAGTAAGAGCTTTCTTAAATTTAAATTAG", "aa": "MVVESNNLLPALKVKHFISFKKLQKIYKKTLHIQLLENECYLYSLINTDHCFIKKTKVKNIQYFALLQDFIEKFKKVRAFLNLN", "aa_hexdigest": "4efa76a6fa17cc2ee86ba8a17d6f4548", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10091.987599999991, "isoelectric_point": 9.732163429260254 }, "id": "OAIKOHHJFB_756", "locus": "GCA000209735_00737" }, { "type": "cds", "sequence": "contig_1", "start": 411941, "stop": 412276, "strand": "-", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XER0", "UniRef:UniRef90_C4XER0" ], "nt": "ATGCAAAGAAAAAAAATCAAAAATATTTTAATTGTTTCTTCTTTTGCTATTCCAATTTCAATGACTATTTCAGCAAGTGGCTGCAATTTAAAAGCTATTGAAAATAAATTCAATAATGAGATAGTGAAATCAAAAGAATGTGTCAAAGAATTTGAAAATAAAAAAGATATTCAAGAAGAAAAATATGAAGAATTAAGAGAAAGTTTAGTGAATTTTTCTAAATATATTGTTCAAGTAGATACTTTAAAACCTGGTCCAGTATCACTAAGAAATAGAAAAATATATCAATATGCAACAAGATTACTTCAAAGTTTTCAACAATTGCATAAAAATTGA", "aa": "MQRKKIKNILIVSSFAIPISMTISASGCNLKAIENKFNNEIVKSKECVKEFENKKDIQEEKYEELRESLVNFSKYIVQVDTLKPGPVSLRNRKIYQYATRLLQSFQQLHKN", "aa_hexdigest": "716207286e8245e04cf5dfd285df89da", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 411854, "stop": 412282, "observations": { "mutation": [ 411877, 411943 ], "stop": [ 411877, 411943 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XER0", "query_cov": 1.0, "subject_cov": 0.7816901408450704, "identity": 1.0, "score": 209.0, "evalue": 5.24e-68, "gene_start": 1, "gene_end": 111, "reference_start": 3, "reference_end": 113, "reference_sequence": "MEMQRKKIKNILIVSSFAIPISMTISASGCNLKAIENKFNNEIVKSKECVKEFENKKDIQEEKYEELRESLVNFSKYIVQVDTLKPGPVSLRNRKIYQYATRLLQSFQQLHKNWYEYILKNGNDENCDDKMTQELWDFYLKK" }, "paralog": false, "identity": 0.9788732394366197, "score": 276.0, "evalue": 7.69e-97, "description": "Internal stop codon at 411877, 411943. Nonsense mutation around 411877, 411943.", "psc": { "uniref90_id": "UniRef90_C4XER0", "product": "Lipoprotein", "uniref50_id": "UniRef50_C4XER0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XER0", "UniRef:UniRef50_C4XER0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XER0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XER0" ], "product": "Lipoprotein" } }, "id": "OAIKOHHJFB_757", "locus": "GCA000209735_00738" }, { "type": "cds", "sequence": "contig_1", "start": 412585, "stop": 413148, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGCTAATAAGCTTATTATTTCTCTTGGTACAGCATTTGCAGCATTGTCTCCAGTTGTATTAAGTGTTGGATGTGGAGGTGGAGGTTCTTCCAACCTAGATTCAATTAATGGTGAAAGTACTCCATCAAATGAAAACTCAGCTTATGCAAACGATAAAAAAGGTTACAATATTAAAGCCAATGACAAAGTTCAAATTGCTACAACTTTCTCTAAAGGTAGAGCTCAATATGAAGCTTTAAATGATATAATCAAAAATTACAACAGATTAATGAAAGATCAACCTGGTTTTAAAGAAGTTGAACAAGTTAGTTTAGGTTCAGGTTATGACGGTCAAGCTGACGTGGTTAGAAAAAAAATAACAGCCAAAGAAGCTCAAATGCCTAACTTACTTCTTAACTATTCATCTTTAGCATCATCTCTTGCCGAAAGTGGAGTATTGTTGAACTTTACAACTAAAGAAAATGGGGGCAAAGAATCAATTTCTAATAGTGATTTTGCAGAATCATTTTCAAGAGCAAATAGAACAACATCAAAAATTAATAATGGTGGTTCATGA", "aa": "MKKANKLIISLGTAFAALSPVVLSVGCGGGGSSNLDSINGESTPSNENSAYANDKKGYNIKANDKVQIATTFSKGRAQYEALNDIIKNYNRLMKDQPGFKEVEQVSLGSGYDGQADVVRKKITAKEAQMPNLLLNYSSLASSLAESGVLLNFTTKENGGKESISNSDFAESFSRANRTTSKINNGGS", "aa_hexdigest": "d2fd6ad824e216f376b320711043b5f1", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19829.867400000006, "isoelectric_point": 9.07793827056885 }, "id": "OAIKOHHJFB_758", "locus": "GCA000209735_00739" }, { "type": "cds", "sequence": "contig_1", "start": 413434, "stop": 413556, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGAAAAATCATCAGCAGTGACTACTTTAGAAGGACCACATGCCTTAGGTATTGATGATATTACAGGATTTATTCAAACTTTAGGATATGCTTCAGTTGGAGCCGATCCTGAATCATGA", "aa": "MFEKSSAVTTLEGPHALGIDDITGFIQTLGYASVGADPES", "aa_hexdigest": "7925ceb9795947445814e282e18d460b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4126.508000000001, "isoelectric_point": 4.0500284194946286 }, "id": "OAIKOHHJFB_759", "locus": "GCA000209735_00740" }, { "type": "cds", "sequence": "contig_1", "start": 413611, "stop": 413850, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAGCTACATCACAAGCTAATAGTAAATTTAAAGAACTTTATGATATGTTTGTTCCTAAATTGAAATCTCAATCTGTTGTTTTCCAACAAAGTGGTGCTTATACATCTAGTGAACAAATAAGACATAACTTTATGTTTGATATTGGTTCAACAGCTGGATATACTCATAACTTTGCTAAAAAAAGCGAAACATCTATTGAAATTAAAAATAGTGAAGGTAAATTCTCTTCTGGATGA", "aa": "MEATSQANSKFKELYDMFVPKLKSQSVVFQQSGAYTSSEQIRHNFMFDIGSTAGYTHNFAKKSETSIEIKNSEGKFSSG", "aa_hexdigest": "17b6ffe1b6509320390a670c815b5cc7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8840.721599999999, "isoelectric_point": 8.00570011138916 }, "id": "OAIKOHHJFB_760", "locus": "GCA000209735_00741" }, { "type": "cds", "sequence": "contig_1", "start": 414073, "stop": 414408, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGAAACCAAAAATAAAGATGATATTAAAACTTTAGATGCAGCTTTAGCAGATGGAAAACTTGTAAAAGTTGGTGAATTTGTTAATAATCAAAATAAAGAAGCTATTTTATACCTTGCTTTTGGTAAAGGTGCTGGTATTTCAGGACTTAAAATTGTTGAAAAAGGTTTAGATTCTCTTCTTGAAAAAGACGAATTCTTTAAATTTGCAACTCCTTCAAAAACAAAAGACACTGATGCTAAAAATGTTTATTATGCACAAGGTCCTTCATTATTTGGAATTAGATCTAGTGATAATGCAATGAATCAAGCAACAAGAATGTTTGTTAAATGA", "aa": "MIETKNKDDIKTLDAALADGKLVKVGEFVNNQNKEAILYLAFGKGAGISGLKIVEKGLDSLLEKDEFFKFATPSKTKDTDAKNVYYAQGPSLFGIRSSDNAMNQATRMFVK", "aa_hexdigest": "df6c7e4fc1bd61b7dac4879eb2adff91", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12183.815599999984, "isoelectric_point": 8.776741981506348 }, "id": "OAIKOHHJFB_761", "locus": "GCA000209735_00742" }, { "type": "cds", "sequence": "contig_1", "start": 414493, "stop": 414690, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTCAGAACAAGCATCATATATTTTTGCTGTTAAAGATTTTGAAAAAACACCAATGACAACAACCAATCCTTACTTACGTGAATGTTTTGAAATGTTTAAAAAAACAATTGAAGATCCAGAACACAATGCTATCTATGAGGAATATGGTGATCCAAATGGTGATGCATTTAGAGGTGCATTAGGAACAGCATGA", "aa": "MFSEQASYIFAVKDFEKTPMTTTNPYLRECFEMFKKTIEDPEHNAIYEEYGDPNGDAFRGALGTA", "aa_hexdigest": "ccd26344ed9bce850cc6cb66f87f58e6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7465.2107, "isoelectric_point": 4.482516288757325 }, "id": "OAIKOHHJFB_762", "locus": "GCA000209735_00743" }, { "type": "cds", "sequence": "contig_1", "start": 414869, "stop": 415279, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGTTTAAAAATAATACTTTAAATTTTTGTTTGAATACAATTGTTCCTTCAATGGCTATTGTAGTTTGTTCTTCATGTCAAAGTAAAGATGACAAAATTTATAATGAAGCTTTTAAAGAAGACATCAAGTTCGATTATGTAGCTTATAATCCAGCTACTGGAAAATATGATATTGTTGACAAAAATATTGAAAATCAATTGACAACTAGTTTGAATAATTTATCAAATATTTCAAGAGAATTTACTTATGATGGTGAAGAATATAAACTTGAAATAGATACAATTTCTCTTGCAAGAACATTTGATAAAAACAAAACTTTTAGTGAAAACTTGAAAGATCCAAACTTTATTAATAATCAAAGGGAATATACTTACAAAATTTTCAAAAAAAATCCTAAAGGTAAATGA", "aa": "MKFKNNTLNFCLNTIVPSMAIVVCSSCQSKDDKIYNEAFKEDIKFDYVAYNPATGKYDIVDKNIENQLTTSLNNLSNISREFTYDGEEYKLEIDTISLARTFDKNKTFSENLKDPNFINNQREYTYKIFKKNPKGK", "aa_hexdigest": "5ab25517ebd08e93a55a015ffe71c73a", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15859.692099999982, "isoelectric_point": 7.595303535461427 }, "id": "OAIKOHHJFB_763", "locus": "GCA000209735_00744" }, { "type": "cds", "sequence": "contig_1", "start": 415310, "stop": 415411, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAGAAATTTGACGTTAAGTTAAATTTAGTTGACAATTTTGACAAAATGGATACTAGAATTGATTATTCAAAATTACAAGCTACAGCTTATGATTGA", "aa": "MTKKFDVKLNLVDNFDKMDTRIDYSKLQATAYD", "aa_hexdigest": "f428e6a48ff83c30e7cda2c2c0749920", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3928.4445000000005, "isoelectric_point": 6.022057151794433 }, "id": "OAIKOHHJFB_764", "locus": "GCA000209735_00745" }, { "type": "cds", "sequence": "contig_1", "start": 415517, "stop": 415717, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00565.23" ], "nt": "ATGGTAGTTGAAACCATTAGAATTCAAGGAGTTGATACTCCTGAAAAAGCTGTTGGCAAAGAAATTGCTCCAATTTTTGAAAAGAGATTCGCGGAAAAATCATCTGAATTTGCTAATGTAAATTTACCAGCTGGTACAAAAGTTAGATTTATTTATTCTAATAAAGATGCTTTTAAAAGAATCGTAGCTCATTTATTTTGA", "aa": "MVVETIRIQGVDTPEKAVGKEIAPIFEKRFAEKSSEFANVNLPAGTKVRFIYSNKDAFKRIVAHLF", "aa_hexdigest": "33a7fc38db56d2043591462342e6f0ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00565.23", "name": "SNase", "length": 61, "aa_cov": 0.9242424242424242, "hmm_cov": 0.9180327868852459, "evalue": 1.757641817189498e-06, "score": 24.523019790649414, "start": 5, "stop": 65 } ], "seq_stats": { "molecular_weight": 7453.5775, "isoelectric_point": 9.519997978210448 }, "id": "OAIKOHHJFB_765", "locus": "GCA000209735_00746" }, { "type": "cds", "sequence": "contig_1", "start": 415742, "stop": 416047, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTATGAATATTCTGCTGCTATTACTAGATTTGGATTTACATTGCCTTATGCTGATGGTACAGAATTAATAAATATGACTAATGCTGGTAAAACTCAACATTATACATTCTTACAAATAGGTGAAGCTTTTGAAGCTGCTATGGCTGAAAAGACAGGATTCTTTAAATGTTTAAGTGATCCTGATGACATATCAAGATATGTTTATTTATCAAAAAGAAATGGTAAATACACCATTTTTCAAAAGAAATCAAATTCAAATGTATTTAAATTTAAAGATAAAGCTTACAAAAACCCTATAAACTAG", "aa": "MYEYSAAITRFGFTLPYADGTELINMTNAGKTQHYTFLQIGEAFEAAMAEKTGFFKCLSDPDDISRYVYLSKRNGKYTIFQKKSNSNVFKFKDKAYKNPIN", "aa_hexdigest": "5661ce40177eeabb1f7757512e22f194", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11612.045599999985, "isoelectric_point": 9.162327384948732 }, "id": "OAIKOHHJFB_766", "locus": "GCA000209735_00747" }, { "type": "cds", "sequence": "contig_1", "start": 416091, "stop": 416531, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGATATTTATTAATAGATTAATTAATCTTTTTAAGAAAAAAACAACCAAGTTTAATAAAGAAGATGAAACAGAATTTTTACGTCTAAAGCAAGAAATAGAATCAAAGAAAAATATTGATCGTAACTCTGCTATAGAATTAAAAAATGTTTTTATCGACTTTGGTGAAACTTTAGCTGTAGATGATGTTAGTTTTAAAATTCCAGAAGGTTCATTAGTAACTCTTTTAGGACCTTCAGGATCAGGTAAAACAACAACTCTTAATGCTATCTCAGGGTTATTAACAATAACAAGTGGGAAGGTGTTTTTTAGAGGAAGAGACGTTACAAGATTAACACCTCAACAAAGGAAATTGGGTTTCGTTTTTCAAAACTATGCCTTATATCCTCATATGAGTGTTTATGACAACATCGCATTCCCTCTTAAAAATGATGCAGATTGA", "aa": "MIFINRLINLFKKKTTKFNKEDETEFLRLKQEIESKKNIDRNSAIELKNVFIDFGETLAVDDVSFKIPEGSLVTLLGPSGSGKTTTLNAISGLLTITSGKVFFRGRDVTRLTPQQRKLGFVFQNYALYPHMSVYDNIAFPLKNDAD", "aa_hexdigest": "90bd6f8ed3309e634846891e505fe558", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 82, "aa_cov": 0.547945205479452, "hmm_cov": 0.9634146341463414, "evalue": 9.233703422644843e-21, "score": 70.98065948486328, "start": 61, "stop": 140 } ], "seq_stats": { "molecular_weight": 16553.856099999983, "isoelectric_point": 9.329300498962404 }, "id": "OAIKOHHJFB_767", "locus": "GCA000209735_00748" }, { "type": "cds", "sequence": "contig_1", "start": 416742, "stop": 417632, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XER3", "UniRef:UniRef90_C4XER3" ], "nt": "ATGTCAAAAGTTCACTATACAGCTGATTTAAGCTTAGCTTCTAAATTGGCTTTAAAAAGCTTTGAAAGCTTGAAAAAGTCAAAACATGATCAACTTAATGCTATTAACGAAGAATACAATCTTCTTAAAAATAACAATAGACTAAAAGCTGAAGAAGAATATAAACAATCAAAATTCTTAACTGATTTTGATAAAAACTTATTAAAAAGAAGCAAACCAAAAAGTATAGAAGAAGTACAAGAAGAACTTGACAAAACTCAAAAACTTATTGAACAAGTTGTTTTAGAAAACCGTAGTGAATTTTCTCATGAAACTCAAAGACAACTTGTTAAAACTGAAAATCAATTAATGAAAAATAATATTTATTACAAATATTACATGAACCTTAAAAAGGCTATTGTTAAATATGAACCATTAATTGATAAATACAAAAAAACTTACAAAGAAGTTAAAAAAGAATATAAAAATTCTGTCCGTAATAATTCAAAATTAAAAAGATTAGTACGCAATGAAAAAATTATGAAAATGTACGCATTAGTTAATTTTGAAAAAATTCAAAATGAAATTTATCAAAAATACAACTTAAAGCAAATTATTGATGAAGATCACAAATTAAAAAATGCGAACTTAACAGATGAAGACAGAAACCAAATTGTTGAATATTCTAAAGATATAATTAGCCTTAAAAAAGCAATTCATAGAGAAGTGCTTGAAGTTGCTGAACGTGTTGAAATTCTTCCAATCTTACAAAAGAAACCAACTCGTCTTTCAGGTGGTCAACAACAACGTGTGGCAATTGCCCGTGCTATTGTTAAAAAACCTCAAATTCTTCTTATGGACGAACCTCTTTCAAACTTGGATGCTAAATTACGTATTTCAACTCGACAATGA", "aa": "MSKVHYTADLSLASKLALKSFESLKKSKHDQLNAINEEYNLLKNNNRLKAEEEYKQSKFLTDFDKNLLKRSKPKSIEEVQEELDKTQKLIEQVVLENRSEFSHETQRQLVKTENQLMKNNIYYKYYMNLKKAIVKYEPLIDKYKKTYKEVKKEYKNSVRNNSKLKRLVRNEKIMKMYALVNFEKIQNEIYQKYNLKQIIDEDHKLKNANLTDEDRNQIVEYSKDIISLKKAIHREVLEVAERVEILPILQKKPTRLSGGQQQRVAIARAIVKKPQILLMDEPLSNLDAKLRISTRQ", "aa_hexdigest": "98f83c74bab74ce76359384bfbb2f653", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 416442, "stop": 417935, "observations": { "mutation": [ 416529, 416640, 417630 ], "stop": [ 416529, 416640, 417630 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XER3", "query_cov": 1.0, "subject_cov": 0.42105263157894735, "identity": 1.0, "score": 522.0, "evalue": 4.43e-180, "gene_start": 1, "gene_end": 296, "reference_start": 223, "reference_end": 518, "reference_sequence": "MKGKNMIFINRLINLFKKKTTKFNKEDETEFLRLKQEIESKKNIDRNSAIELKNVFIDFGETLAVDDVSFKIPEGSLVTLLGPSGSGKTTTLNAISGLLTITSGKVFFRGRDVTRLTPQQRKLGFVFQNYALYPHMSVYDNIAFPLKNDADWQNKVLDRKTEAIINIRNIYLKSLGASDLEIKQINEAWKIYKSIYRETQSELANYRVELREKFDKAFTDYKMSKVHYTADLSLASKLALKSFESLKKSKHDQLNAINEEYNLLKNNNRLKAEEEYKQSKFLTDFDKNLLKRSKPKSIEEVQEELDKTQKLIEQVVLENRSEFSHETQRQLVKTENQLMKNNIYYKYYMNLKKAIVKYEPLIDKYKKTYKEVKKEYKNSVRNNSKLKRLVRNEKIMKMYALVNFEKIQNEIYQKYNLKQIIDEDHKLKNANLTDEDRNQIVEYSKDIISLKKAIHREVLEVAERVEILPILQKKPTRLSGGQQQRVAIARAIVKKPQILLMDEPLSNLDAKLRISTRQWIRDIQQKLGITTVFVTHDQEEAMSISDIVICMSMSKVQQIGSPMELYNKPRNKFVARFLGMPEMGMFDSKYKDGKLTVLGVEIPGIKLNDVDFKDLSVGVRAEDFEIKNRKQDAQFSGVVKAAENFGKESKLIVTLENGENINFLVDNDYNYKAKDEIYFNIPKHRLHIFDKLTEERIEYEIKK" }, "paralog": false, "identity": 0.993963782696177, "score": 940.0, "evalue": 0.0, "description": "Internal stop codon at 416529, 416640, 417630. Nonsense mutation around 416529, 416640, 417630.", "psc": { "uniref90_id": "UniRef90_C4XER3", "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_C4XER3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XER3", "UniRef:UniRef50_C4XER3" ] }, "pscc": { "uniref50_id": "UniRef50_C4XER3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XER3" ], "product": "ABC transporter domain-containing protein" } }, "id": "OAIKOHHJFB_768", "locus": "GCA000209735_00749" }, { "type": "cds", "sequence": "contig_1", "start": 417699, "stop": 418187, "strand": "+", "frame": 3, "gene": null, "product": "TOBE domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C8186B" ], "nt": "ATGTCAATTAGTGACATTGTTATCTGTATGTCAATGTCTAAAGTTCAACAAATTGGTTCACCAATGGAACTTTACAACAAACCACGTAACAAATTTGTAGCTAGATTTTTAGGAATGCCAGAAATGGGCATGTTCGACTCTAAATATAAAGATGGAAAACTTACTGTTTTAGGTGTTGAAATTCCTGGAATCAAATTAAACGATGTTGACTTCAAAGATCTTAGTGTTGGTGTTAGAGCCGAAGACTTTGAAATTAAAAATAGAAAGCAAGATGCACAATTCAGTGGCGTAGTTAAAGCTGCTGAAAACTTTGGTAAAGAAAGCAAATTAATAGTAACTTTAGAAAATGGTGAAAATATCAACTTTTTAGTTGACAATGACTATAATTACAAAGCTAAAGATGAAATTTATTTCAACATACCAAAACATCGTCTTCACATTTTTGACAAGTTAACAGAAGAAAGAATTGAATATGAAATCAAAAAATAA", "aa": "MSISDIVICMSMSKVQQIGSPMELYNKPRNKFVARFLGMPEMGMFDSKYKDGKLTVLGVEIPGIKLNDVDFKDLSVGVRAEDFEIKNRKQDAQFSGVVKAAENFGKESKLIVTLENGENINFLVDNDYNYKAKDEIYFNIPKHRLHIFDKLTEERIEYEIKK", "aa_hexdigest": "803def7f3858fe188acb441811a784e7", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI0024C8186B", "query_cov": 0.808641975308642, "subject_cov": 0.9492753623188406, "identity": 0.534, "score": 130.0, "evalue": 7.14e-36, "valid": false, "product": "TOBE domain-containing protein", "uniref50_id": "UniRef50_UPI0024C8186B" }, "pscc": { "uniref50_id": "UniRef50_UPI0024C8186B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C8186B" ], "product": "TOBE domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_769", "locus": "GCA000209735_00750" }, { "type": "cds", "sequence": "contig_1", "start": 418327, "stop": 418569, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCAACTTTACTTGTTATTACAGCATTTACATTAGTTCCTTTTATAATAAACATTATTCAATCATTTACTGTACAAAATGCTAAAAATGGCGTTTTACATAATTTTGAATCTCTATTTAAGGAACCAAAATTTGCTGTAGGGGTTAGAAACTCAATTTTATATGCTATTGTTGTTCTACCTTTTGTTATGATGGTATCTTTATTAATATCTTCATGTATCGCAAACGTTTATCGTAAGTGA", "aa": "MPTLLVITAFTLVPFIINIIQSFTVQNAKNGVLHNFESLFKEPKFAVGVRNSILYAIVVLPFVMMVSLLISSCIANVYRK", "aa_hexdigest": "10afcda7012ffb74a434f2cbb33af95f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8944.721199999996, "isoelectric_point": 9.744928169250485 }, "id": "OAIKOHHJFB_770", "locus": "GCA000209735_00751" }, { "type": "cds", "sequence": "contig_1", "start": 418816, "stop": 419196, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTCAGTTGACAAAAACCTCTATAAATCAGCCTCAATCGACGGAATTGGACCAGTTAAACAATTCTTTACAATTACCTTGCCTTCTATTAAATCAACTACTACTTTCTTAGTAACAATGGGAATTATAAACGGAATAAAAGTCTTTCCTCTTGCATTATTCAATAATAGACCTGAAGAAGCAGTTTTAAAAGGTGCTTCAACCTTAATGATCTTCGTTTACTACTACACAAGTCTTGGTGGAGCAGAAAACTTATCATTAGCTGCTGCAGGTTCTATATTGTTATTTATTATTGGTATAGTTTACTCAACTATTATTAGAGGGGGATTTGGAACAATAATGAGAGTATCATTAAACTTAGGAGAAAGCAATGTTTGA", "aa": "MLSVDKNLYKSASIDGIGPVKQFFTITLPSIKSTTTFLVTMGIINGIKVFPLALFNNRPEEAVLKGASTLMIFVYYYTSLGGAENLSLAAAGSILLFIIGIVYSTIIRGGFGTIMRVSLNLGESNV", "aa_hexdigest": "8b9d57ddc326d6fd3e561ff740d2ff53", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13488.749299999985, "isoelectric_point": 9.346255683898928 }, "id": "OAIKOHHJFB_771", "locus": "GCA000209735_00752" }, { "type": "cds", "sequence": "contig_1", "start": 419294, "stop": 419452, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTACTTGGTTTATTTCTTAAATTATTGGCATTAACATTTTTTGCTCTTATTATTTTGTTCCCATTCTTCTTTATGTTGATGATTTCTTTGATGCCTGAAACTCAAGCAAAAACATTACAATCATCATTCTCAATAATTCCTAAATCATTAACTTGA", "aa": "MLLGLFLKLLALTFFALIILFPFFFMLMISLMPETQAKTLQSSFSIIPKSLT", "aa_hexdigest": "c9463c2ae26de4d5c9eac5ef58a0baf9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5928.348499999999, "isoelectric_point": 9.700251579284668 }, "id": "OAIKOHHJFB_772", "locus": "GCA000209735_00753" }, { "type": "cds", "sequence": "contig_1", "start": 419711, "stop": 419944, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCACTTAAAAATAATTTCATAGGTGCTATGGTTATTATTGCTGTACCATTTGTGGCTTCAATCTTCAATGCTTTAATGTTTAAAAATGCATTTGAATCGATTCCTTCAAGAATTAAAGAAGTTGCTTTAGTCGATGGAGCTGCGGGTGCTAAATATCTGTTTAAAATAGCTATACCTATGGTTACACCAACAACTTTAACAGTGGTAATTCTAACAGCCTTAGCTGCTTGA", "aa": "MALKNNFIGAMVIIAVPFVASIFNALMFKNAFESIPSRIKEVALVDGAAGAKYLFKIAIPMVTPTTLTVVILTALAA", "aa_hexdigest": "b984adcf4cc5919a3b14d1bcd3edaec6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8140.814599999996, "isoelectric_point": 9.69799518585205 }, "id": "OAIKOHHJFB_773", "locus": "GCA000209735_00754" }, { "type": "cds", "sequence": "contig_1", "start": 420065, "stop": 420172, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTGGTGCCGTACTTGTTATTATACCAATGTTTATTTTCTACTTTGCCTTTAGAAAAAGAATTATGAATGCTATATCTCGTCAAGGAAGTACTATTAAAGGTTAG", "aa": "MAGAVLVIIPMFIFYFAFRKRIMNAISRQGSTIKG", "aa_hexdigest": "cc64338f5b148f1a69f97488c4a299f7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3948.8095999999996, "isoelectric_point": 11.733906745910645 }, "id": "OAIKOHHJFB_774", "locus": "GCA000209735_00755" }, { "type": "cds", "sequence": "contig_1", "start": 420471, "stop": 420647, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAAAAATGAAATTTAAAAAATTAATGTTTATTGCTGCTTCAACTTTAGCCTTAGGAGTTGCACCTGCAATTACAATGTCATGTAATTTCTATATTAAATCTAATCCATTCTTAGAATATCTTAAAGACAAATCAGAAAATTTAAGTTTTTATAAGAAAACTCCATATTGA", "aa": "MNKKMKFKKLMFIAASTLALGVAPAITMSCNFYIKSNPFLEYLKDKSENLSFYKKTPY", "aa_hexdigest": "c912bcb3fc7340d47c9f219846e6fca3", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6705.986799999999, "isoelectric_point": 9.76465549468994 }, "id": "OAIKOHHJFB_775", "locus": "GCA000209735_00756" }, { "type": "cds", "sequence": "contig_1", "start": 420690, "stop": 421718, "strand": "+", "frame": 3, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XER7", "UniRef:UniRef90_C4XER7" ], "nt": "TTGGCTTACAACTTTTTTGCTCCACATCATGCTAAAGTTTTACGTTACGATTCAAATGGTGCTTTCCCTTATTTAACTTCAACAAGGGAATCAACTTTATATGATTCAAACTTTAGTGAAAAAGGCATTTTTGATAACCTTTCTTATACAGAAAGTCAACAAGTTAAAAAACTTGAAGAATTTAAAAATACAAAAGAATACATTAAACAACATCCAAATGAAGTTGGCAAATACTTAACAAAATATTTTTATCAATTAACTAATAAACAAGCTGAAGATGTTTTTATGTATTACTTCAACAATACTCAATATGGTTACTACGTTTTAACGAATTATTATCGTATGTTCAAAGTTGAAAATGATTTTATTTACGATGGAAGTGAAAAACCTCTTAAAAACATTTCAACAATTTCAGACGGTACTAATAAACGTTATTTTGACATTGTTAATCTATATGCATTAGAACATTTAGGTGATTTTATTAAAGAAAATAAAAATTTAATTAATGAAAATTTTGAAGACTTTTTTATTAAATTCTTAAATGAAAGTAAAAAATATGGTCTAAGAATATCTTCAAAAAACGATAGTTTAGTAGCACAAGAAGGTATGCAAAATGCTTTTGCTCCTGATTACTATTTAAGAAATCAAGATCGTAAACAAGAAGACTTAAAGGATGCCGCATTATTTTACAAAAATGCTGATTTAAACCCTGAAGGACAAATTTATAAAGACTTTATGAAAAATCCTGTTGAGTTTATTAAAAAACATCCAAACGTTATTAAAGCTGATGCTTCAGAAACAAAAATTAATGACCGTACAAATAAAAATTACACAATTACTGTTCTTAATAAAATAAAAAGCGAAATTAATAAAATAAAAAATTATGGTGTTAATGAAGAAACCATTTTAAGAGCACTATTTACAATTTTAGAATTTCATGGATTAAAAGATTATCAACTTGGATTTGCATACAATAAAACAACTAAAAATCTTGAATTTTATTTTGAAAAATTAGTTAATAACAAATGA", "aa": "MAYNFFAPHHAKVLRYDSNGAFPYLTSTRESTLYDSNFSEKGIFDNLSYTESQQVKKLEEFKNTKEYIKQHPNEVGKYLTKYFYQLTNKQAEDVFMYYFNNTQYGYYVLTNYYRMFKVENDFIYDGSEKPLKNISTISDGTNKRYFDIVNLYALEHLGDFIKENKNLINENFEDFFIKFLNESKKYGLRISSKNDSLVAQEGMQNAFAPDYYLRNQDRKQEDLKDAALFYKNADLNPEGQIYKDFMKNPVEFIKKHPNVIKADASETKINDRTNKNYTITVLNKIKSEINKIKNYGVNEETILRALFTILEFHGLKDYQLGFAYNKTTKNLEFYFEKLVNNK", "aa_hexdigest": "cbd715eb545ffe891fd68025ccd9d655", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 420459, "stop": 421967, "observations": { "mutation": [ 420645, 421716, 421839 ], "stop": [ 420645, 421716, 421839 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XER7", "query_cov": 1.0, "subject_cov": 0.6812749003984063, "identity": 0.997, "score": 675.0, "evalue": 1.08e-242, "gene_start": 1, "gene_end": 342, "reference_start": 78, "reference_end": 419, "reference_sequence": "MRRIMNKKMKFKKLMFIAASTLALGVAPAITMSCNFYIKSNPFLEYLKDKSENLSFYKKTPYWLQKNAKDKTSNQLELAYNFFAPHHAKVLRYDSNGAFPYLTSTRESTLYDSNFSEKGIFDNLSYTESQQVKKLEEFKNTKEYIKQHPNEVGKYLTKYFYQLTNKQAEDVFMYYFNNTQYGYYVLTNYYRMFKVENDFIYDGSEKPLKNISTISDGTNKRYFDIVNLYALEHLGDFIKENKNLINENFEDFFIKFLNESKKYGLRISSKNDSLVAQEGMQNAFAPDYYLRNQDRKQEDLKDAALFYKNADLNPEGQIYKDFMKNPVEFIKKHPNVIKADASETKINDRTNKNYTITVLNKIKSEINKIKNYGVNEETILRALFTILEFHGLKDYQLGFAYNKTTKNLEFYFEKLVNNKWKIFSLSNFFDNLRKKFDRTNPVDEEFTKDKLITYDAFPSDWTFDLPNTVTKDDVDKMSIYLENGVKELKFTLTKIKQYSNIK" }, "paralog": false, "identity": 0.9920318725099602, "score": 1004.0, "evalue": 0.0, "description": "Internal stop codon at 420645, 421716, 421839. Nonsense mutation around 420645, 421716, 421839.", "psc": { "uniref90_id": "UniRef90_C4XER7", "product": "Lipoprotein", "uniref50_id": "UniRef50_C4XER7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XER7", "UniRef:UniRef50_C4XER7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XER7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XER7" ], "product": "Lipoprotein" } }, "id": "OAIKOHHJFB_776", "locus": "GCA000209735_00757" }, { "type": "cds", "sequence": "contig_1", "start": 422048, "stop": 422740, "strand": "-", "frame": 1, "gene": "rmuC", "product": "DNA recombination protein RmuC", "db_xrefs": [ "KEGG:K09760", "SO:0001217", "UniRef:UniRef50_C4XER8", "UniRef:UniRef90_C4XER8" ], "nt": "ATGAACCCAAATAGCGAAAGAGTTGACTTTGTTATTAAAATGCCTAATGGAGAAGCTAACTCTCAAAATGATCGTTACTTGCCTATTGATTGTAAGTTTCCACTTGATTCTTTTAATGCATATCAAGATGCAGACTCTAAAGAAGAAAGAATTAAAGCTCAAATTAACTTTAGAAAAGTTATTCTCGAACAAGCTAAAAGTATTAAAAGTAAATACATTATTAAAAATGTAACTACCAAGCAAGCAATTATGTATCTACCATCTGAAGCTATTTTTGCTTTAGCAGTTAGTGACAATGAATTGGTTTCAGCATTGAATAAAGATCATGATATTATGTTGGCTGGACCAAGTACAATTTTGATGATTATTCAAACCATTGCTTATGCAAATTATGGAATTAACATAAAACAAAATATTGACATTCTTTTAGATGCATTCTTATTTATTCGTCAAAGTGAAGATAATATTTATACCAAAATTGAACAAAGTATCAAGAAAAATGATAAAACAGGAGTTGAATTAAATAGAGCATTAAAAATAGTCGATAGAGTAAAGAAAAGAATTCAAAATTCTATTGATAGTGCACGAAAAAAAGGTATTATAAAAGAAGATTATGACATTGATAAAGTTGCAATGAATATGCCTATGATTGAAGATTTAAGTGATGAAAATGATGATAAAGAAGAAGAATAA", "aa": "MNPNSERVDFVIKMPNGEANSQNDRYLPIDCKFPLDSFNAYQDADSKEERIKAQINFRKVILEQAKSIKSKYIIKNVTTKQAIMYLPSEAIFALAVSDNELVSALNKDHDIMLAGPSTILMIIQTIAYANYGINIKQNIDILLDAFLFIRQSEDNIYTKIEQSIKKNDKTGVELNRALKIVDRVKKRIQNSIDSARKKGIIKEDYDIDKVAMNMPMIEDLSDENDDKEEE", "aa_hexdigest": "fd352c1047aa627bcd1878a783a3777e", "start_type": "ATG", "rbs_motif": null, "genes": [ "rmuC" ], "truncated": "3-prime", "pseudogene": { "start": 422048, "stop": 423040, "observations": { "mutation": [ 422815 ], "stop": [ 422815 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XER8", "query_cov": 1.0, "subject_cov": 0.5287356321839081, "identity": 1.0, "score": 431.0, "evalue": 2.12e-149, "gene_start": 1, "gene_end": 230, "reference_start": 206, "reference_end": 435, "reference_sequence": "MEIGILIVVILTMILAISSIVVSFILYKKNKTEVIDQDKMTNNIKNQITEDLSSQFVKFNKNVDEKIANSFNMINKNFSDLREEIDKKMKSFDDLVIKEVQIIKGAIDEKFENEVAKKMEENFKNIKENMENLDKNLVRFETLQDSVNSLQKTFDGTKSRGNYGEFNLKSILNEHLSKDLWAAQVDLKKIAKKGLDEAAAVDFEDMNPNSERVDFVIKMPNGEANSQNDRYLPIDCKFPLDSFNAYQDADSKEERIKAQINFRKVILEQAKSIKSKYIIKNVTTKQAIMYLPSEAIFALAVSDNELVSALNKDHDIMLAGPSTILMIIQTIAYANYGINIKQNIDILLDAFLFIRQSEDNIYTKIEQSIKKNDKTGVELNRALKIVDRVKKRIQNSIDSARKKGIIKEDYDIDKVAMNMPMIEDLSDENDDKEEE" }, "paralog": false, "identity": 0.996969696969697, "score": 612.0, "evalue": 6.17e-223, "description": "Internal stop codon at 422815. Nonsense mutation around 422815.", "psc": { "uniref90_id": "UniRef90_C4XER8", "product": "DNA recombination protein RmuC", "uniref50_id": "UniRef50_C4XER8", "kegg_orthology_id": "K09760", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XER8", "UniRef:UniRef50_C4XER8", "KEGG:K09760" ] }, "pscc": { "uniref50_id": "UniRef50_C4XER8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XER8" ], "product": "DNA recombination protein RmuC" } }, "id": "OAIKOHHJFB_777", "locus": "GCA000209735_00758" }, { "type": "cds", "sequence": "contig_1", "start": 422813, "stop": 423355, "strand": "-", "frame": 1, "gene": "rmuC", "product": "DNA recombination protein RmuC", "db_xrefs": [ "KEGG:K09760", "SO:0001217", "UniRef:UniRef50_C4XER8", "UniRef:UniRef90_C4XER8" ], "nt": "ATGGAAATAGGAATTCTAATTGTAGTAATATTGACTATGATTTTAGCTATTAGTAGTATTGTCGTTAGTTTCATCTTATATAAAAAAAATAAGACAGAAGTTATCGATCAAGACAAAATGACAAATAATATTAAAAATCAAATAACTGAAGATTTGTCTAGTCAATTTGTCAAATTCAATAAAAATGTTGATGAAAAAATTGCCAATTCATTCAACATGATTAATAAAAACTTTAGTGATTTAAGAGAAGAAATTGACAAAAAAATGAAATCGTTTGATGATTTAGTAATTAAGGAAGTGCAAATAATTAAAGGTGCTATTGATGAAAAATTTGAAAATGAAGTAGCTAAAAAGATGGAAGAAAACTTTAAAAATATTAAAGAAAACATGGAAAATCTTGACAAGAATTTAGTACGTTTTGAGACCTTACAAGATTCAGTTAATTCATTACAAAAAACTTTTGATGGAACAAAAAGTCGCGGTAATTATGGTGAGTTTAATTTAAAGAGTATTTTGAATGAACATTTATCAAAAGACTTGTGA", "aa": "MEIGILIVVILTMILAISSIVVSFILYKKNKTEVIDQDKMTNNIKNQITEDLSSQFVKFNKNVDEKIANSFNMINKNFSDLREEIDKKMKSFDDLVIKEVQIIKGAIDEKFENEVAKKMEENFKNIKENMENLDKNLVRFETLQDSVNSLQKTFDGTKSRGNYGEFNLKSILNEHLSKDL", "aa_hexdigest": "a8011aee3ee43a45f6749d607917feee", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "rmuC" ], "truncated": "5-prime", "pseudogene": { "start": 422510, "stop": 423355, "observations": { "mutation": [ 422815 ], "stop": [ 422815 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XER8", "query_cov": 1.0, "subject_cov": 0.41379310344827586, "identity": 1.0, "score": 300.0, "evalue": 1.42e-98, "gene_start": 1, "gene_end": 180, "reference_start": 1, "reference_end": 180, "reference_sequence": "MEIGILIVVILTMILAISSIVVSFILYKKNKTEVIDQDKMTNNIKNQITEDLSSQFVKFNKNVDEKIANSFNMINKNFSDLREEIDKKMKSFDDLVIKEVQIIKGAIDEKFENEVAKKMEENFKNIKENMENLDKNLVRFETLQDSVNSLQKTFDGTKSRGNYGEFNLKSILNEHLSKDLWAAQVDLKKIAKKGLDEAAAVDFEDMNPNSERVDFVIKMPNGEANSQNDRYLPIDCKFPLDSFNAYQDADSKEERIKAQINFRKVILEQAKSIKSKYIIKNVTTKQAIMYLPSEAIFALAVSDNELVSALNKDHDIMLAGPSTILMIIQTIAYANYGINIKQNIDILLDAFLFIRQSEDNIYTKIEQSIKKNDKTGVELNRALKIVDRVKKRIQNSIDSARKKGIIKEDYDIDKVAMNMPMIEDLSDENDDKEEE" }, "paralog": false, "identity": 0.99644128113879, "score": 515.0, "evalue": 1.29e-185, "description": "Internal stop codon at 422815. Nonsense mutation around 422815.", "psc": { "uniref90_id": "UniRef90_C4XER8", "product": "DNA recombination protein RmuC", "uniref50_id": "UniRef50_C4XER8", "kegg_orthology_id": "K09760", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XER8", "UniRef:UniRef50_C4XER8", "KEGG:K09760" ] }, "pscc": { "uniref50_id": "UniRef50_C4XER8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XER8" ], "product": "DNA recombination protein RmuC" } }, "id": "OAIKOHHJFB_778", "locus": "GCA000209735_00759" }, { "type": "cds", "sequence": "contig_1", "start": 423535, "stop": 423708, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATAGTTAAATTAACTAAAAAAGAATATAAAGGTAAAGAATTCTGTGTCCAATATAAAACAAAAGGCTATTACTTTATAGAACATACTAAAAATGGATTTAGTTTTGTTTACAAAAAATTTGATCAAGAAAAAACTTTTGGTTACTGTGACAAGTTTTTTCAAGATTGA", "aa": "MKIVKLTKKEYKGKEFCVQYKTKGYYFIEHTKNGFSFVYKKFDQEKTFGYCDKFFQD", "aa_hexdigest": "e7e1c937be06c6942f6b226f649f2982", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7032.078200000002, "isoelectric_point": 9.369270896911619 }, "id": "OAIKOHHJFB_779", "locus": "GCA000209735_00760" }, { "type": "cds", "sequence": "contig_1", "start": 423865, "stop": 424059, "strand": "+", "frame": 1, "gene": null, "product": "N-acetyltransferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A358PH68" ], "nt": "ATGAAAAAAATGATTGCAAAAGCTAAAACAACACCTGCTAGAATGATTGTTTTAGAAACTGAAACATGTAATGAAAAAGCAATAAGATTTTATCAAAAATTCAATTTTGAAGTTATTGGATTTGACTTGTATGCATATACAAATGAAGATCCAAAAGAACATGAAGTTAGAGTTGAAATGGGTTTAAAAATCTAG", "aa": "MKKMIAKAKTTPARMIVLETETCNEKAIRFYQKFNFEVIGFDLYAYTNEDPKEHEVRVEMGLKI", "aa_hexdigest": "05d928382105f62a47e001beb02f031c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1H9U7D2", "query_cov": 0.953125, "subject_cov": 0.8356164383561644, "identity": 0.557, "score": 78.2, "evalue": 9.01e-18, "valid": false, "product": "Acetyltransferase (GNAT) family protein", "uniref50_id": "UniRef50_A0A358PH68" }, "pscc": { "uniref50_id": "UniRef50_A0A358PH68", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A358PH68" ], "product": "N-acetyltransferase domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_780", "locus": "GCA000209735_00761" }, { "type": "cds", "sequence": "contig_1", "start": 424103, "stop": 424387, "strand": "+", "frame": 2, "gene": null, "product": "DJ-1/PfpI domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDU0", "UniRef:UniRef90_C4XES0" ], "nt": "ATGAAATTATTAGTTTTAACTCATCCAATGTTTAATGACATTGAATTAACAACAGTCCTTGGTTGTTTAATTAGAAGTGGCAAAATGAGCAAAATTACTCTTTATAATCACAAGCATAAAAGTGCTACAGGACAATTTGGAGTAACTACTTTGGCTTTAGAAAATAAAGTTAATTTAGATGAATACGATGCTATCTTTATTCCAGGCGGTAAAGGCGCACAAGAATTAAGAAAAGATCCTGAAAGTTTAAAAGTAATAGATTACTTTATTAAAAATGATAAATGA", "aa": "MKLLVLTHPMFNDIELTTVLGCLIRSGKMSKITLYNHKHKSATGQFGVTTLALENKVNLDEYDAIFIPGGKGAQELRKDPESLKVIDYFIKNDK", "aa_hexdigest": "3013309bd069b81796dec4c8369696a4", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 424103, "stop": 424642, "observations": { "mutation": [ 424385 ], "stop": [ 424385 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XES0", "query_cov": 1.0, "subject_cov": 0.5251396648044693, "identity": 1.0, "score": 191.0, "evalue": 1.25e-60, "gene_start": 1, "gene_end": 94, "reference_start": 1, "reference_end": 94, "reference_sequence": "MKLLVLTHPMFNDIELTTVLGCLIRSGKMSKITLYNHKHKSATGQFGVTTLALENKVNLDEYDAIFIPGGKGAQELRKDPESLKVIDYFIKNDKWVCAICDAPNVLYENHFIDDKVKYSSFPIIPLVGGKNRNEKMVTVNNKIITGRCAASSMEFGLTLVKEFFGEDVYEPIRKGMFGA" }, "paralog": false, "identity": 0.994413407821229, "score": 360.0, "evalue": 7.42e-130, "description": "Internal stop codon at 424385. Nonsense mutation around 424385.", "psc": { "uniref90_id": "UniRef90_C4XES0", "product": "DJ-1/PfpI domain-containing protein", "uniref50_id": "UniRef50_A0A3P8MDU0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XES0", "UniRef:UniRef50_A0A3P8MDU0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MDU0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDU0" ], "product": "Putative intracellular protease/amidase (Putative glutaminase)" } }, "id": "OAIKOHHJFB_781", "locus": "GCA000209735_00762" }, { "type": "cds", "sequence": "contig_1", "start": 424508, "stop": 424642, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAACAGTAAATAACAAAATTATTACTGGTCGTTGTGCAGCAAGTTCAATGGAATTTGGTTTAACTTTAGTTAAAGAATTCTTTGGTGAAGATGTTTACGAGCCTATTCGTAAAGGAATGTTTGGAGCTTAA", "aa": "MVTVNNKIITGRCAASSMEFGLTLVKEFFGEDVYEPIRKGMFGA", "aa_hexdigest": "db2917e9cc46a20c64f75ee3c74e2c13", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4858.635200000001, "isoelectric_point": 6.068665122985839 }, "id": "OAIKOHHJFB_782", "locus": "GCA000209735_00763" }, { "type": "cds", "sequence": "contig_1", "start": 424936, "stop": 425097, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAATAGTTATACAAATCGAAACAACTTACTTTCATTCAAATACAATCAATTAGCTGAAAGAGTTGGCAAGATTAAAATTAGCAAAAAATCATACAAATATTCTTTTGATAAACTAGAAAATTTAATTAAATATTTCTCATTAAATTACAAAAGATGA", "aa": "MLNSYTNRNNLLSFKYNQLAERVGKIKISKKSYKYSFDKLENLIKYFSLNYKR", "aa_hexdigest": "eb7899aac6b2d12912ba15f767e07369", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6474.4464, "isoelectric_point": 10.054183006286618 }, "id": "OAIKOHHJFB_783", "locus": "GCA000209735_00764" }, { "type": "cds", "sequence": "contig_1", "start": 425509, "stop": 426213, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XES1", "UniRef:UniRef90_C4XES1" ], "nt": "ATGCAAACAAATAATCAACAATTAAAAGAGCAAATTATCGTTTCAAACGATAATCTTTTAATTCGCAATGATACCTTTTTATTTAAAACAAATAACAAAGAAAAAATAGAACTCTCATCAATCTTGCGACTATTGCAAGCTATAAATAAAACTATTTTAAAAAGCAATAGTAACATTTTATTAAGCTTTGAAGGTCAAGAATTTTATAATGAAAATATTCAATATTTTAGCTCTTATTTTATCAAAAATAATCATAATGTTTTTAAGTATCACAATAACTTTTCAATTAGTTTGATTTTAGATGAATATGCCTATAAAAAAGCTTCATATGACTTTTTAATCAAATTTAAAAATATTAAAAATGATAACTATATTACAATTCAAATATTTGATAAAAACTTTGAAATTTTGACCTCTGAAAACTTACAAAAAATTTATGATTATTATCAATTAAACAAATGGCAAAATAAAGAAATTGTCATCAAAGATCCTAAACTAGTTAATGAATTTGAACTGATTGATGAATTAGCTTCAAAAGAACAAATTCTTAAAGCTTTCGTTAATGTTAAACAAAGATATAAAACATTAAGTTACTTTGCTAGTGACAATAAATTTAGTCAAAGTATTGGTACAGAACTATTAAATAATTATCAAAATAAATACATTACAAACAATAAAAATATTTCGCTTTTAAACCTAAAATGA", "aa": "MQTNNQQLKEQIIVSNDNLLIRNDTFLFKTNNKEKIELSSILRLLQAINKTILKSNSNILLSFEGQEFYNENIQYFSSYFIKNNHNVFKYHNNFSISLILDEYAYKKASYDFLIKFKNIKNDNYITIQIFDKNFEILTSENLQKIYDYYQLNKWQNKEIVIKDPKLVNEFELIDELASKEQILKAFVNVKQRYKTLSYFASDNKFSQSIGTELLNNYQNKYITNNKNISLLNLK", "aa_hexdigest": "8537e5c8cd94727749f5b0d6e3142810", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 425206, "stop": 426228, "observations": { "mutation": [ 425394, 425484, 425511 ], "stop": [ 425394, 425484, 425511 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XES1", "query_cov": 1.0, "subject_cov": 0.45525291828793774, "identity": 1.0, "score": 446.0, "evalue": 5.89e-154, "gene_start": 1, "gene_end": 234, "reference_start": 6, "reference_end": 239, "reference_sequence": "MIGNFMQTNNQQLKEQIIVSNDNLLIRNDTFLFKTNNKEKIELSSILRLLQAINKTILKSNSNILLSFEGQEFYNENIQYFSSYFIKNNHNVFKYHNNFSISLILDEYAYKKASYDFLIKFKNIKNDNYITIQIFDKNFEILTSENLQKIYDYYQLNKWQNKEIVIKDPKLVNEFELIDELASKEQILKAFVNVKQRYKTLSYFASDNKFSQSIGTELLNNYQNKYITNNKNISLLNLKWLTFFAASSWYRENKIQNIFHFDINSNLNVCLRLNDKFKWLNSHELVLIYLDFFLEEIKRNGIDISNSYVVVPQNTTYQILELLKQYKVKYYFYDLKNINKQLKNESCLFAYIDNKFIANPRYSTQFNNYYFFICLIWMLNSYTNRNNLLSFKYNQLAERVGKIKISKKSYKYSFDKLENLIKYFSLNYKRWFTAFNAYRVWNDHKYMLFKLINNKKHQMIFYWDDVKGKLVIEYQMCCNFENISNTVWWDKLKLQILLFVLIKKAQKHKIEKEE" }, "paralog": false, "identity": 0.9882352941176471, "score": 664.0, "evalue": 5.25e-242, "description": "Internal stop codon at 425394, 425484, 425511. Nonsense mutation around 425394, 425484, 425511.", "psc": { "uniref90_id": "UniRef90_C4XES1", "uniref50_id": "UniRef50_C4XES1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XES1", "UniRef:UniRef50_C4XES1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XES1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XES1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 27937.44870000004, "isoelectric_point": 9.023269081115721 }, "id": "OAIKOHHJFB_784", "locus": "GCA000209735_00765" }, { "type": "cds", "sequence": "contig_1", "start": 426248, "stop": 426355, "strand": "-", "frame": 1, "gene": "gyrA", "product": "DNA gyrase subunit A", "db_xrefs": [ "EC:5.6.2.2", "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0006259", "GO:0006265", "KEGG:K02469", "SO:0001217", "UniParc:UPI0001A8C215", "UniRef:UniRef100_C4XES2", "UniRef:UniRef50_B3PN30", "UniRef:UniRef90_A0A3P8MFB5" ], "nt": "ATGGCTGCAACTTCTGTTGTTCAAAGCATTTATGAACAAAGTCAAGAAATCAACTTAGATGATAACTCAAACGATGAAATTGATATTGATTCAAACAATGAAGAATAA", "aa": "MAATSVVQSIYEQSQEINLDDNSNDEIDIDSNNEE", "aa_hexdigest": "182db44856b17f41fdb52df12d9b036a", "start_type": "ATG", "rbs_motif": "AGxAG", "ups": { "uniparc_id": "UPI0001A8C215", "uniref100_id": "UniRef100_C4XES2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C215", "UniRef:UniRef100_C4XES2" ] }, "ips": { "uniref100_id": "UniRef100_C4XES2", "uniref90_id": "UniRef90_A0A3P8MFB5" }, "psc": { "uniref90_id": "UniRef90_A0A3P8MFB5", "gene": "gyrA", "product": "DNA gyrase subunit A", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_B3PN30", "kegg_orthology_id": "K02469", "go_ids": [ "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0006259", "GO:0006265" ] }, "pscc": { "uniref50_id": "UniRef50_B3PN30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN30" ], "product": "DNA gyrase subunit A" }, "genes": [ "gyrA" ], "id": "OAIKOHHJFB_785", "locus": "GCA000209735_00766" }, { "type": "cds", "sequence": "contig_1", "start": 426496, "stop": 429027, "strand": "-", "frame": 2, "gene": "gyrA", "product": "DNA gyrase subunit A", "db_xrefs": [ "EC:5.6.2.2", "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0006259", "GO:0006265", "KEGG:K02469", "SO:0001217", "UniRef:UniRef50_B3PN30", "UniRef:UniRef90_A0A3P8MFB5" ], "nt": "ATGAGTTCAAAAGAACAAGATAAAGAAAAAGAATACAAAAGCAAACTTAATCAAGAAGAAGAAAAAGAAAATGCAATCGAAGAATTAGATGAAGATTACATTCCTGATGAAGAGCTTTTTGTTGCTTTTAAACCACAAAAAGAAGAAACTAAAGTTATTGAAGGGGAGGAAGAAGAAGTTCCTCAAAATAAAGACAACTATGTAGTTCAACCACAACTTTTAGATGCACCTAAAGATGGTATTCATCCAGTTGAAGTTCACAAAGAAATGAAAAACTCATTCTTAGAATATGCAATGAGTGTTATTGTTTCTCGTGCTTTACCAGATGCTCGTGATGGACTTAAACCAGTACATAGACGTATTCTTTTTGATATGAATGAATTAGGAATTACATTTGGATCGCAACATAGAAAAAGCGCTCGTATTGTCGGGGACGTTTTAGGTAAGTACCACCCACATGGCGACAGTTCAGTTTATGAAGCTATGGTTCGTATGGCGCAAGATTTTAGTATGCGTTATCCTTTAGTTGATGGTCACGGTAACTTTGGATCTATTGATGGTGATGAAGCTGCTGCGATGCGTTATACTGAAGCAAGAATGAGCAAATTAGCTGCTGAAATGCTTGAAGGTATTAAAAAAGATACAGTAGATTTTGTTGATAACTATGATGCTAGTGAAAAAGAACCTTCAGTATTACCATCAAGATTCCCTAACCTTTTAGTTTCAGGTGGTAGTGGTATTGCTGTTGGTATGGCAACAAGTATTCCACCTCACAACTTAGGTGAAACTATTGATGCAACTATTGCTTTAGCTAACAATCCAGAAATTGATATTTATGGCTTAATGGAATTTTTACCTGGTCCAGACTTTCCTACTGGAGCTATGATTTTAGGCAATGCAGGTATTAAAGATGCATACTTAACAGGCAAGGGATCCATTCCAATCAGATCAATAGCTAAAATAGAAGAATACTCAAATGGTAAAAGCAAAATTATTGTTACTGAAATTCCTTATGAAGTTAAGAAAACTGCAATTATTGAAAAAATAGCTGAATTAGTTAAAGATAAACAAATCGAAGGTATCGCCGACTTACGTGATGAAAGTAGCCGTGAAGGTATTAGAATTGTTATTGACGTTAAAAAGAATGTTAATCCTCATGTTTTATTAAACAAGCTTTATCGTCAAACAAACTTACAAGTAAACTACAATGTTAACTTTGTTGCTTTAGTTGATGGTGAACCTAAAGTTTTAAACTTAAAACAAGCTTTAGAAGTTTACTTAAGACACCAAGAAGAAGTAGTGACTAGAAGATTAAAATTTGATTTAATTAAAGCTCAAGACCGTCTTCACATTTTAGAAGGTCTTAAAATTGCAGTTGAAAATATTGATGAAGTTATTGCAATTATTAAGAAATCTAAAACAGATGGTGAAGCTCAAGCTAACTTAGCAAAAAGATTTAATTTAACTGAAAAACAAACAAAAGCTATTGTTGACATGCGTTTAGGTCGTCTTACAGGACTTGCTATTGAAAACATGCTTGAAGAAATGGCAGAGTTAAGAAAAGAAATTGAAAGAATTGAATTTATTTTGGCTAACCATAGTGAATTAATTAAATTAATTGTTAAAGAATTAACTGAAATTAAAGAAAAATACAATGACAAACGTCGCACACAAATTAACTATGAAATTCAAGGTACTATTACTGATGAAGACTTGATTCCTCAACGTGACATTGTTATTACAACCAGCGCCAAAGGCTTTGTAAAAAGAATGGACTTAGAAGAATACAAAACTCAACGTCGTGGTGGAGTTGGTATTGGTACTATGAAAACATATGACGATGATGATATTGCCTCAATATTAACAACTACAACTCACACCGACTTACTATTATTCTCAAATCATGCTCGTGTCTACCGTGTTAGAGCTCATGAAATTCCAGAATTATCTCGTCAAAGTAAAGGTGTAGCATTCATTAATATTATTCCTTCACTTCGTGTTAAAGAAGGTGAAAGAATTGTTTCAATGCTTCCAGTAGACACTTACGATGAAGATCACTACTTATTTACAGCAACTAAAAATGGTATTATCAAGAAAACTAGATTAACTGAATACGAAAGAATTAACGCTAATGGTAAATATGCTTTCAACTTACGTGAAGGCGATGAATTAATTAGAGCAATGATTATTACTGATGAAGACTTAATTCTTTTAGCAAATGATGAAGAAAGAATTGTTAAATTCCAAGCTATTGACTTTAGACCAATTAGTAGAACTGCAACTGGTGTTAAAGGTATAACACTTGATAGCCATCAAACAGTTTCAAGCACTTCAACAAGTCAAGAAGGTGAATTAATCTTAACTATTGGTAAAAAAGGTTTTGGAAAACTTACTCACCACTCACTATTTAGATTAATTAAACGTGGTGGTAAAGGTGTTAAAGGAATTAATAGCAAATTAGCAGGCGACATTGTTTTTGCTAGATTCGTAAATCCTCAAGATGAACTTTTATTATTACAACAAGTGGTTTAA", "aa": "MSSKEQDKEKEYKSKLNQEEEKENAIEELDEDYIPDEELFVAFKPQKEETKVIEGEEEEVPQNKDNYVVQPQLLDAPKDGIHPVEVHKEMKNSFLEYAMSVIVSRALPDARDGLKPVHRRILFDMNELGITFGSQHRKSARIVGDVLGKYHPHGDSSVYEAMVRMAQDFSMRYPLVDGHGNFGSIDGDEAAAMRYTEARMSKLAAEMLEGIKKDTVDFVDNYDASEKEPSVLPSRFPNLLVSGGSGIAVGMATSIPPHNLGETIDATIALANNPEIDIYGLMEFLPGPDFPTGAMILGNAGIKDAYLTGKGSIPIRSIAKIEEYSNGKSKIIVTEIPYEVKKTAIIEKIAELVKDKQIEGIADLRDESSREGIRIVIDVKKNVNPHVLLNKLYRQTNLQVNYNVNFVALVDGEPKVLNLKQALEVYLRHQEEVVTRRLKFDLIKAQDRLHILEGLKIAVENIDEVIAIIKKSKTDGEAQANLAKRFNLTEKQTKAIVDMRLGRLTGLAIENMLEEMAELRKEIERIEFILANHSELIKLIVKELTEIKEKYNDKRRTQINYEIQGTITDEDLIPQRDIVITTSAKGFVKRMDLEEYKTQRRGGVGIGTMKTYDDDDIASILTTTTHTDLLLFSNHARVYRVRAHEIPELSRQSKGVAFINIIPSLRVKEGERIVSMLPVDTYDEDHYLFTATKNGIIKKTRLTEYERINANGKYAFNLREGDELIRAMIITDEDLILLANDEERIVKFQAIDFRPISRTATGVKGITLDSHQTVSSTSTSQEGELILTIGKKGFGKLTHHSLFRLIKRGGKGVKGINSKLAGDIVFARFVNPQDELLLLQQVV", "aa_hexdigest": "ee7dc7f191ebfb4828e8d31e739d1879", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_A0A3P8MFB5", "query_cov": 0.9952550415183867, "subject_cov": 0.906047516198704, "identity": 0.998, "score": 1563.0, "evalue": 0.0, "valid": true, "gene": "gyrA", "product": "DNA gyrase subunit A", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_B3PN30", "kegg_orthology_id": "K02469", "go_ids": [ "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0006259", "GO:0006265" ] }, "pscc": { "uniref50_id": "UniRef50_B3PN30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN30" ], "product": "DNA gyrase subunit A" }, "genes": [ "gyrA" ], "id": "OAIKOHHJFB_786", "locus": "GCA000209735_00767" }, { "type": "cds", "sequence": "contig_1", "start": 429445, "stop": 429549, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAATTAATAGAATGATAACGAAATTTGATGAAGAAAATCCAGGTATTTTAGTCAAAATGCAAAAGGCTCATATTGCTCCATCTTTTTTAGCTTATATTTGA", "aa": "MTINRMITKFDEENPGILVKMQKAHIAPSFLAYI", "aa_hexdigest": "52ceff1930d05be9e6b2bd35a8ec370e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3921.6504999999997, "isoelectric_point": 8.211289787292483 }, "id": "OAIKOHHJFB_787", "locus": "GCA000209735_00768" }, { "type": "cds", "sequence": "contig_1", "start": 429690, "stop": 429848, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACATAACTTTTTTATCAAGTGTAATATTTTTACGATTTAAAATCAATATGACTATTCCAATTAATGGATTAATTGCATGTACAATTGTGCTTAAAGCTAATCTAAAGGAACCATACTCTGATCCAAATATTTTTGGAAAAATTAAAGTTCAATAA", "aa": "MYITFLSSVIFLRFKINMTIPINGLIACTIVLKANLKEPYSDPNIFGKIKVQ", "aa_hexdigest": "99409788cfdf9cb41ca922d09144a65b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5918.127000000001, "isoelectric_point": 9.655059242248537 }, "id": "OAIKOHHJFB_788", "locus": "GCA000209735_00769" }, { "type": "cds", "sequence": "contig_1", "start": 429961, "stop": 430107, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATAGTGATATTGTAGTTTATGGCTTTTTAAACTTTATTGAACCATTTTTTTATCGCGGAGGTAATATTGCAATAGTTATTACATTAGACTTATTAATGATTATAATAGCAGTCTTTGTACCTATTGGCTTAAGTTATTTTTGA", "aa": "MDSDIVVYGFLNFIEPFFYRGGNIAIVITLDLLMIIIAVFVPIGLSYF", "aa_hexdigest": "2925e74b6286f27787552784d1df5c43", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5431.448099999998, "isoelectric_point": 4.0500284194946286 }, "id": "OAIKOHHJFB_789", "locus": "GCA000209735_00770" }, { "type": "cds", "sequence": "contig_1", "start": 430396, "stop": 430887, "strand": "+", "frame": 1, "gene": null, "product": "Small ribosomal subunit protein uS4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPZ2" ], "nt": "ATGAGATATAGAGGTCCCGTTTTCAAGAAAAGCCGTCGTTATGGATTTTCAATTTTAGAAACAGGTAAAGAATTTTCAAAAGGTAGAAAAAGAACTTATGCTCCAGGTCAACATGGTGACAAACGTGTTAAATTAAGTGACTATGGACTTCACCTTTATGAAAAACAAAAAGTTAAATACTTATATGGTTTAAATGAAAAACAAATGCACAAATACTACATTCGTGCAACCAAAATTAAAGGTGTTACAGGTACAGTATTGTTACAACTTTTAGAAAGTCGTTTAGACAACTTAGTTTATAGAGCAGGTTTTGCTTCAACACGTCGTCAAGCACGTCAATTAGTAAGCCACGGCCACTTTACATTAAATGGTCACAAAGCCGACATTCCTTCAATGAATGTTGAAGTTGGCTCAAAAATCCAATTAAAAGAAAAATCCCAAAAGAATAAAATTATTGAAGCTAACCTTAAAGATCGTTCAGCTGCTAAATGA", "aa": "MRYRGPVFKKSRRYGFSILETGKEFSKGRKRTYAPGQHGDKRVKLSDYGLHLYEKQKVKYLYGLNEKQMHKYYIRATKIKGVTGTVLLQLLESRLDNLVYRAGFASTRRQARQLVSHGHFTLNGHKADIPSMNVEVGSKIQLKEKSQKNKIIEANLKDRSAAK", "aa_hexdigest": "fee83a092c3b0cd7e14f7ad20f689366", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A974WKF8", "query_cov": 1.0, "subject_cov": 0.8232323232323232, "identity": 0.816, "score": 258.0, "evalue": 8.85e-86, "valid": false, "gene": "rpsD", "product": "30S ribosomal protein S4", "uniref50_id": "UniRef50_Q9PPZ2", "kegg_orthology_id": "K02986", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPZ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPZ2" ], "product": "Small ribosomal subunit protein uS4" }, "genes": [], "id": "OAIKOHHJFB_790", "locus": "GCA000209735_00771" }, { "type": "cds", "sequence": "contig_1", "start": 431012, "stop": 431251, "strand": "+", "frame": 2, "gene": "rpmE", "product": "50S ribosomal protein L31", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354566.1", "SO:0001217", "UniParc:UPI0001A8C219", "UniRef:UniRef100_C4XES6", "UniRef:UniRef50_A0A449BAA4", "UniRef:UniRef90_A0A3P8KXC8" ], "nt": "ATGAAAAAAAATATTCATCCACAATATTTTACAGTTGAAGCAACATGTTCAACATGTGGTAAAAAATTTCAATTTGGTTCAACTAAAAAATCAATTTCTGTCGATGTATGTTCAGGTTGCCACGTAGTTTATACAGGCGACAGAACAAAAACAAAAGCAACTGGTATGATTGACAAATTCAACCAACGTTTAGCAAAACAAAAAGCTGCTATTGAAAAATCAAAAGGTAAGAAAAAATAA", "aa": "MKKNIHPQYFTVEATCSTCGKKFQFGSTKKSISVDVCSGCHVVYTGDRTKTKATGMIDKFNQRLAKQKAAIEKSKGKKK", "aa_hexdigest": "a74ba465a9352977cd327abffc417088", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C219", "ncbi_nrp_id": "WP_013354566.1", "uniref100_id": "UniRef100_C4XES6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C219", "RefSeq:WP_013354566.1", "UniRef:UniRef100_C4XES6" ] }, "ips": { "uniref100_id": "UniRef100_C4XES6", "uniref90_id": "UniRef90_A0A3P8KXC8" }, "psc": { "uniref90_id": "UniRef90_A0A3P8KXC8", "gene": "rpmE", "product": "50S ribosomal protein L31", "uniref50_id": "UniRef50_A0A449BAA4", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449BAA4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449BAA4" ], "product": "Large ribosomal subunit protein bL31" }, "genes": [ "rpmE" ], "id": "OAIKOHHJFB_791", "locus": "GCA000209735_00772" }, { "type": "cds", "sequence": "contig_1", "start": 431438, "stop": 432232, "strand": "+", "frame": 2, "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "db_xrefs": [ "GO:0003677", "GO:0006355", "KEGG:K03705", "SO:0001217", "UniRef:UniRef50_N9VB78", "UniRef:UniRef90_C4XES7" ], "nt": "ATGGCTAATATAAATTTAAATGACGAAAAAAAGAAAATTCTTAAATATGTTATTGAATCTTACATTGAAGAAGGGATCCCGATTGGTTCTCAATTATTGGTTGACAAATATAAATTGAACATTTCTTCATCCAAAGTTAGATACATTTTAAATGAATTTGAAGAAGCTGGACTTTTAGAAAAAACACACACTTCAAGTGGACGTATTCCTTCAAATCTTGGTTATAACTATTATGTAAAAAATCTAGTAAATAAAGATTCAAACATACTATATGAAAAATTAAAAGATATTTTTGCTCGCCGTCGTGTTTCAATTGATCAAACAGTTAAAGAAGCGGCAAAAACCATCTGTGAAACAGTTGGAATAACACTTGTTACTTCAGAAAGTAATGAAGATGCAACACTTAAAAGTATTCAACTTGTACCACTTAATACTGCTGAAGCAACTGTTATTTTAGTAACCTCTTATGGTGAAGTTACATCTAAAACAATAACAATTGACTTAAACCTTGTAACAATGTCAGATTTGAAAATAGCTATTAGAATTTTCAAAGAAAGATTAATCGATGTACCAATTTTAAAACTTAAAAGTACAGCAGAAGCATTGACCCCAATTCTTGCTGATACAATTAAAAACTATGAAAGTATTTTGCAAAGTTTTGTAACAAATGTTTTTGATTTCCAAATCAAGAATGAAAATGTGATTTATGGAAAAGATAATATAATTTTAGCAGACGATATTTCAAGACAAGATTTAAGTAAAATACTTCATTTAATCGAAACTAAATCAATATGA", "aa": "MANINLNDEKKKILKYVIESYIEEGIPIGSQLLVDKYKLNISSSKVRYILNEFEEAGLLEKTHTSSGRIPSNLGYNYYVKNLVNKDSNILYEKLKDIFARRRVSIDQTVKEAAKTICETVGITLVTSESNEDATLKSIQLVPLNTAEATVILVTSYGEVTSKTITIDLNLVTMSDLKIAIRIFKERLIDVPILKLKSTAEALTPILADTIKNYESILQSFVTNVFDFQIKNENVIYGKDNIILADDISRQDLSKILHLIETKSI", "aa_hexdigest": "78cd355c64ac915be62402a5bae62818", "start_type": "ATG", "rbs_motif": null, "genes": [ "hrcA" ], "truncated": "3-prime", "pseudogene": { "start": 431435, "stop": 432460, "observations": { "mutation": [ 432230 ], "stop": [ 432230 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XES7", "query_cov": 1.0, "subject_cov": 0.7741935483870968, "identity": 1.0, "score": 488.0, "evalue": 9.56e-173, "gene_start": 1, "gene_end": 264, "reference_start": 2, "reference_end": 265, "reference_sequence": "MMANINLNDEKKKILKYVIESYIEEGIPIGSQLLVDKYKLNISSSKVRYILNEFEEAGLLEKTHTSSGRIPSNLGYNYYVKNLVNKDSNILYEKLKDIFARRRVSIDQTVKEAAKTICETVGITLVTSESNEDATLKSIQLVPLNTAEATVILVTSYGEVTSKTITIDLNLVTMSDLKIAIRIFKERLIDVPILKLKSTAEALTPILADTIKNYESILQSFVTNVFDFQIKNENVIYGKDNIILADDISRQDLSKILHLIETKSIWQTIESEIDEDENIKIAICDDHSTFISKKLETQSKIKEIALVGTSRMDYEKGFSALKALEEFIENKPCKSKDEGDK" }, "paralog": false, "identity": 0.9941348973607038, "score": 647.0, "evalue": 1.01e-237, "description": "Internal stop codon at 432230. Nonsense mutation around 432230.", "psc": { "uniref90_id": "UniRef90_C4XES7", "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "uniref50_id": "UniRef50_N9VB78", "kegg_orthology_id": "K03705", "go_ids": [ "GO:0003677", "GO:0006355" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XES7", "UniRef:UniRef50_N9VB78", "KEGG:K03705", "GO:0003677", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_N9VB78", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9VB78" ], "product": "Heat-inducible transcription repressor HrcA" } }, "id": "OAIKOHHJFB_792", "locus": "GCA000209735_00773" }, { "type": "cds", "sequence": "contig_1", "start": 432368, "stop": 432460, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTATGAAAAAGGATTTAGTGCTCTTAAAGCCTTAGAGGAATTTATTGAAAATAAACCATGCAAATCAAAAGATGAAGGAGATAAATAA", "aa": "MDYEKGFSALKALEEFIENKPCKSKDEGDK", "aa_hexdigest": "c21a28a2550c58b0a55b5ed381902f00", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3450.8446000000013, "isoelectric_point": 4.812637138366699 }, "id": "OAIKOHHJFB_793", "locus": "GCA000209735_00774" }, { "type": "cds", "sequence": "contig_1", "start": 432460, "stop": 433713, "strand": "+", "frame": 1, "gene": "grpE", "product": "Protein GrpE", "db_xrefs": [ "KEGG:K03687", "RefSeq:WP_041594211.1", "SO:0001217", "UniParc:UPI00030DD68F", "UniRef:UniRef100_C4XES8", "UniRef:UniRef50_C4XES8", "UniRef:UniRef90_C4XES8" ], "nt": "ATGAAAGTAGTTCCTAATTTAAACAAATTTAAAACTGGTGAAGGTATTATTGCAAATATATCAGTTTATAATAGCTCAAATGCTTATCAAAAACAATTATCAAAAGAAAAAGTAAGCTTTGTTTTAGGTGAAAATACATTTTTACCAAACTTTGACAACAATATTGTTGAACAAACTGTTAGACCAGTTTTAGATTTTGTTGTTACATTTCCAAAAAGCTATGAAGTAGCAGAATTCAAAGACAAAACATTTAGATTTGTTATTGAAATTTTAGACTACCAAGATAGTGAATATGTTAAAGCATACAAACAAATGTTAGCTTTAAGAGAAGAAATACAAAAATTAAATGTTGCTTCAGGCAATCATTTAAAAGAACTTGAAGAATTAAAAGCTAAAAATGAAAAACAAATGAGTGAAATTAGTAATTTAAAAACTGAAAATGATAAACATACTAAAGATTCTGCTGATCTTAAAAATCAATATCAAGAACAAGAAAAAACTATTCAAAAACTTATTGAAGATAACAAAAAATTTATAGCAGACAATAATGCTTTAAAAGAAGATAATGAAAACAAAGGTAAACAAATTAAAGAATTAATGTCAGGAAAAACCACAAAATTAATGGAGCAAACTAGTGAACATGAAGCAAAAGGTGTTAATCCTGCAGTTTTAAGAAAACAAGTTGATGTTTTAAGAAAACAACTTGAAGAACAATCAATTGAATTAAAAGAACTTAGAAAAAATAAAGCTCCTGAAGCTGTTCAAAGAGTGACGGTTCCTAAAGAAGAAGTTGACAAAATTAAACAATATGCATTACAAAAATTCTTCGAAGATTTTTCAACTCATTATACAACTTTAAAAGGTGCTGTTAAAGCTGGTGCTAAATCAGAAAACAGTAGTGTTAAAAACTATGTAGTTGGTTTTGAAATGATTTTAAACTTAATCAATGGCGTTTTAGAAAAACACTGTATTAAAGCAATTGAACCAAAAATTGGTGATGAATTTGATCCTAATACTCAAAAAGTTCTTGAAGTTGAAGAATGCAAAGATAAAAAACACAATACAATTGTTAAAGTTAGTGCTATTGGATTTAAATTACATGATAGAGTTATTAAACCTGCTTTAGTTGTAATTGCTCAAGATTCAAGTGCAAAAGACAAAAAACTAGCAGCGGAAACTAAAAAACCAGAAGCTCCAAAGGCTAAAGAAACAACTAAGAAAACTGAAACTAAAAAAACAACAACCAAAAAATAA", "aa": "MKVVPNLNKFKTGEGIIANISVYNSSNAYQKQLSKEKVSFVLGENTFLPNFDNNIVEQTVRPVLDFVVTFPKSYEVAEFKDKTFRFVIEILDYQDSEYVKAYKQMLALREEIQKLNVASGNHLKELEELKAKNEKQMSEISNLKTENDKHTKDSADLKNQYQEQEKTIQKLIEDNKKFIADNNALKEDNENKGKQIKELMSGKTTKLMEQTSEHEAKGVNPAVLRKQVDVLRKQLEEQSIELKELRKNKAPEAVQRVTVPKEEVDKIKQYALQKFFEDFSTHYTTLKGAVKAGAKSENSSVKNYVVGFEMILNLINGVLEKHCIKAIEPKIGDEFDPNTQKVLEVEECKDKKHNTIVKVSAIGFKLHDRVIKPALVVIAQDSSAKDKKLAAETKKPEAPKAKETTKKTETKKTTTKK", "aa_hexdigest": "f5a5d193eeaf45d7dd2bb034c86121a8", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00030DD68F", "ncbi_nrp_id": "WP_041594211.1", "uniref100_id": "UniRef100_C4XES8", "db_xrefs": [ "SO:0001217", "UniParc:UPI00030DD68F", "RefSeq:WP_041594211.1", "UniRef:UniRef100_C4XES8" ] }, "ips": { "uniref100_id": "UniRef100_C4XES8", "uniref90_id": "UniRef90_C4XES8" }, "psc": { "uniref90_id": "UniRef90_C4XES8", "product": "Protein GrpE", "uniref50_id": "UniRef50_C4XES8", "kegg_orthology_id": "K03687" }, "pscc": { "uniref50_id": "UniRef50_C4XES8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XES8" ], "product": "Protein GrpE" }, "genes": [ "grpE" ], "id": "OAIKOHHJFB_794", "locus": "GCA000209735_00775" }, { "type": "cds", "sequence": "contig_1", "start": 433933, "stop": 434301, "strand": "+", "frame": 1, "gene": null, "product": "Large conductance mechanosensitive channel", "db_xrefs": [ "KEGG:K03282", "SO:0001217", "UniRef:UniRef50_A0A077LBI7", "UniRef:UniRef90_C4XES9" ], "nt": "ATGTTCATGTTAGCAATTGGTCTTTTATTAGGTACCGCATTTAATGCTGTTGTTTCATCTTTAGCAAATGATGTTATTATGGCAGCAATTGCGAAAGCATTCAATGTGGATGAAGTTAAAGACTTAAAAGCTGGCCCAATTTTAATTGGCAAATTCTTAGCTGCTTTAATTTCATTTTTGATTGTGGCAACAGTTATATTTATATTGTTAGTTGTTGTCTTTTTAGTTAAAAATGCTATAGAAACTAGAAAATTTAAAAAGAACCCACCTGTTGCGGAAGAACCAAAACCTACAACTGAAGAACTTATTTTAGCTGAAATTAAAAAACTAAATGAAAGACTTGAAAAACTAGAACAACCTAAAAAATAA", "aa": "MFMLAIGLLLGTAFNAVVSSLANDVIMAAIAKAFNVDEVKDLKAGPILIGKFLAALISFLIVATVIFILLVVVFLVKNAIETRKFKKNPPVAEEPKPTTEELILAEIKKLNERLEKLEQPKK", "aa_hexdigest": "4071d75cdfd5135218d201d4f67d5683", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XES9", "query_cov": 1.0, "subject_cov": 0.8776978417266187, "identity": 1.0, "score": 209.0, "evalue": 1.06e-67, "valid": true, "product": "Large conductance mechanosensitive channel", "uniref50_id": "UniRef50_A0A077LBI7", "kegg_orthology_id": "K03282" }, "pscc": { "uniref50_id": "UniRef50_A0A077LBI7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077LBI7" ], "product": "Large-conductance mechanosensitive channel" }, "genes": [], "id": "OAIKOHHJFB_795", "locus": "GCA000209735_00776" }, { "type": "cds", "sequence": "contig_1", "start": 434414, "stop": 434521, "strand": "+", "frame": 2, "gene": null, "product": "HTH-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEC1", "UniRef:UniRef90_A0AB32XCG9" ], "nt": "ATGGTTATTTTTGACTTATTTAACTATAATAAAGGACTATACGGTTATAGACGTATTACATTTGTTTTAAGAAATAAAGAAGTAATGATTAATTATAAAAAGGATTAA", "aa": "MVIFDLFNYNKGLYGYRRITFVLRNKEVMINYKKD", "aa_hexdigest": "09eee22433129b8a8b122a6da5976d4e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 434417, "stop": 434565, "observations": { "deletion": [ 434519 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XCG9", "query_cov": 0.9428571428571428, "subject_cov": 0.6111111111111112, "identity": 0.848, "score": 63.9, "evalue": 7.18e-13, "gene_start": 2, "gene_end": 34, "reference_start": 4, "reference_end": 36, "reference_sequence": "MKKIIFDLFNYNKGLYGYRRITFALRNKGIMINHKKVQKLIKSLNLFGKTLRKK" }, "paralog": false, "identity": 0.8235294117647058, "score": 80.1, "evalue": 3.99e-22, "description": "Frameshift due to deletion around 434519.", "psc": { "uniref90_id": "UniRef90_A0AB32XCG9", "uniref50_id": "UniRef50_C4XEC1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XCG9", "UniRef:UniRef50_C4XEC1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEC1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEC1" ], "product": "HTH-like domain-containing protein" } }, "id": "OAIKOHHJFB_796", "locus": "GCA000209735_00777" }, { "type": "cds", "sequence": "contig_1", "start": 434753, "stop": 434893, "strand": "+", "frame": 2, "gene": null, "product": "Integrase catalytic domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET0", "UniRef:UniRef90_C4XET0" ], "nt": "ATGAATTTTAGGACCAGAAAGATTTTAGGTTATTCAATTACTAAATCTCCAAATTTAAGAATGATAGGAAAAATGTTAGAAAATGTTGAAGAAAATGGTCATAGTTTAAACAATGTATTAATACATTCTGATCAAGGATGA", "aa": "MNFRTRKILGYSITKSPNLRMIGKMLENVEENGHSLNNVLIHSDQG", "aa_hexdigest": "8f2563d043fc78156bf87b6c69c55c2d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 434600, "stop": 434947, "observations": { "mutation": [ 434690, 434891 ], "stop": [ 434690, 434891 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XET0", "query_cov": 1.0, "subject_cov": 0.4, "identity": 1.0, "score": 94.0, "evalue": 7.5e-24, "gene_start": 1, "gene_end": 46, "reference_start": 52, "reference_end": 97, "reference_sequence": "MPNLLLDKENITEDFFRYKRNFSNEKYLDIWGTDVTEFKLINEEKIYFSPVMNFRTRKILGYSITKSPNLRMIGKMLENVEENGHSLNNVLIHSDQGWQYTHQDYIDYLKEKNAI" }, "paralog": false, "identity": 0.9739130434782609, "score": 228.0, "evalue": 4.25e-79, "description": "Internal stop codon at 434690, 434891. Nonsense mutation around 434690, 434891.", "psc": { "uniref90_id": "UniRef90_C4XET0", "product": "Integrase catalytic domain-containing protein", "uniref50_id": "UniRef50_C4XET0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XET0", "UniRef:UniRef50_C4XET0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XET0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET0" ], "product": "Integrase catalytic domain-containing protein" } }, "id": "OAIKOHHJFB_797", "locus": "GCA000209735_00778" }, { "type": "cds", "sequence": "contig_1", "start": 435293, "stop": 435490, "strand": "+", "frame": 2, "gene": null, "product": "Class IIb bacteriocin, lactobin A/cerein 7B family", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MFB6", "UniRef:UniRef90_C4XET1" ], "nt": "ATGAAAGACAAACAAATTCAATTAAAACCTTTAACAGAAGAACAAGAAGCAAACATTCATGGTGGATCAATTATTGCTACCATTTCAGGAATTGTTGGCATTTTAGCAACAATAGCTACAACAGTAAATGGAATTGTTAAAAGTCATAAATCACCTAAAGGCGAATTTAAAGACAAAACAGGAAGTCAAATTAAATGA", "aa": "MKDKQIQLKPLTEEQEANIHGGSIIATISGIVGILATIATTVNGIVKSHKSPKGEFKDKTGSQIK", "aa_hexdigest": "2b2fb9a0f6957ba4c47116ceac72589a", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 435254, "stop": 435532, "observations": { "mutation": [ 435488 ], "stop": [ 435488 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XET1", "query_cov": 1.0, "subject_cov": 0.6989247311827957, "identity": 1.0, "score": 127.0, "evalue": 4.98e-37, "gene_start": 1, "gene_end": 65, "reference_start": 14, "reference_end": 78, "reference_sequence": "MLPKRKVKNERRTMKDKQIQLKPLTEEQEANIHGGSIIATISGIVGILATIATTVNGIVKSHKSPKGEFKDKTGSQIKWDNSPATNGNIFYAW" }, "paralog": false, "identity": 0.9782608695652174, "score": 178.0, "evalue": 1.06e-59, "description": "Internal stop codon at 435488. Nonsense mutation around 435488.", "psc": { "uniref90_id": "UniRef90_C4XET1", "uniref50_id": "UniRef50_A0A3P8MFB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XET1", "UniRef:UniRef50_A0A3P8MFB6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MFB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MFB6" ], "product": "Class IIb bacteriocin, lactobin A/cerein 7B family" } }, "id": "OAIKOHHJFB_798", "locus": "GCA000209735_00779" }, { "type": "cds", "sequence": "contig_1", "start": 435812, "stop": 436432, "strand": "+", "frame": 2, "gene": "infC", "product": "translation initiation factor IF-3", "db_xrefs": [ "GO:0003743", "GO:0005829", "GO:0006413", "GO:0016020", "GO:0032790", "GO:0043022", "SO:0001217", "UniParc:UPI0001E330C1", "UniRef:UniRef100_C4XET2", "UniRef:UniRef50_Q05426", "UniRef:UniRef90_Q05426" ], "nt": "ATGAAAAAACCAACAGCAGAACACATGATTAATGAAAATATTCCATTTCAAAAAGTGTTTGTAGTTGGCGCAAATGGTGAAAAAGTTGGTGTTTTATCAACACGTGAGGCCATTGAAATGGCCAAAGAACAAAGATTAGATTTAGTTCTAATTAGTGTTCAACCTAAACCAATTGCCCGTATTTTAGATTATGGAAAATTTAAATACGAAAGAAAAAAGAAACAAAAAGCAGCTAAAGAAAAACAAACTGTAATTCAAAATCGTCAAATTAGACTTACTCCACTTATTGGAGAACATGACTTAAATGTTAAAGCAAGAAAAGCTAAAGAATTCTTATTAGATGGTGATAGAATTAAAGTAAGTTTGAAATTTAGAGGTCGTGAATCGGCTCGTCCTGAACTAGGTCACAAAACTTTAGAAAAATTTTACAAAGCTGTAGAAGACTTAGCTGATATTGCTAAAGAAGCAGAATTAGTTAATGATCGTTTCTTAGATATGTATTTGCAACCAAATAAAATCAAGGTTAATAAATATAGAAAAGAACATAACTTAGTAGATAAAAATTCAGATAGTCAAGATAAATGTGCTTCAGAAGAAGACACAAATGAAGGAGAGCAATAA", "aa": "MKKPTAEHMINENIPFQKVFVVGANGEKVGVLSTREAIEMAKEQRLDLVLISVQPKPIARILDYGKFKYERKKKQKAAKEKQTVIQNRQIRLTPLIGEHDLNVKARKAKEFLLDGDRIKVSLKFRGRESARPELGHKTLEKFYKAVEDLADIAKEAELVNDRFLDMYLQPNKIKVNKYRKEHNLVDKNSDSQDKCASEEDTNEGEQ", "aa_hexdigest": "a0f29309140a45a2b7ddf14c8c28bc66", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E330C1", "uniref100_id": "UniRef100_C4XET2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330C1", "UniRef:UniRef100_C4XET2" ] }, "ips": { "uniref100_id": "UniRef100_C4XET2", "uniref90_id": "UniRef90_Q05426" }, "psc": { "uniref90_id": "UniRef90_Q05426", "gene": "infC", "product": "translation initiation factor IF-3", "uniref50_id": "UniRef50_Q05426", "go_ids": [ "GO:0003743", "GO:0005829", "GO:0006413", "GO:0016020", "GO:0032790", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_Q05426", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q05426" ], "product": "Translation initiation factor IF-3" }, "genes": [ "infC" ], "id": "OAIKOHHJFB_799", "locus": "GCA000209735_00780" }, { "type": "cds", "sequence": "contig_1", "start": 436434, "stop": 436622, "strand": "+", "frame": 3, "gene": "rpmI", "product": "50S ribosomal protein L35", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "KEGG:K02916", "RefSeq:WP_013526795.1", "SO:0001217", "UniParc:UPI000016F938", "UniRef:UniRef100_Q05428", "UniRef:UniRef50_Q05428", "UniRef:UniRef90_Q05428" ], "nt": "ATGCCTAAAATGAAGACTAAGAGTGCTCTTAAGAAACGTATTAAGATCACTGGAACTGGAAAAATTATGCGTGAACAAGCGTATCGTTCTCACTTAAGCCAAAATAAAACAACTAAACAAAAACGTCAAGCTCGTAAATCAGTACAAATGCACTCATCAGATGTAAAAAGATTTAAAGCATTAATCTAA", "aa": "MPKMKTKSALKKRIKITGTGKIMREQAYRSHLSQNKTTKQKRQARKSVQMHSSDVKRFKALI", "aa_hexdigest": "cdc21f893c75ed1d684dbb06b25c3927", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI000016F938", "ncbi_nrp_id": "WP_013526795.1", "uniref100_id": "UniRef100_Q05428", "db_xrefs": [ "SO:0001217", "UniParc:UPI000016F938", "RefSeq:WP_013526795.1", "UniRef:UniRef100_Q05428" ] }, "ips": { "uniref100_id": "UniRef100_Q05428", "uniref90_id": "UniRef90_Q05428" }, "psc": { "uniref90_id": "UniRef90_Q05428", "gene": "rpmI", "product": "50S ribosomal protein L35", "uniref50_id": "UniRef50_Q05428", "kegg_orthology_id": "K02916", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "genes": [ "rpmI" ], "id": "OAIKOHHJFB_800", "locus": "GCA000209735_00781" }, { "type": "cds", "sequence": "contig_1", "start": 437007, "stop": 437312, "strand": "+", "frame": 3, "gene": null, "product": "Transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET5", "UniRef:UniRef90_C4XET5" ], "nt": "ATGCAAAATAATAATATTAAAATTCTATTTAAAATGAACCACAAATTCCGGACTTTTGGAAAGGGTTCATTTTTATACAATTTAAATTTAGAAAATAAAAAAGGAAGAAAATCTAAAATAAATTTAGACGCTCAAAAGGCAACAAATTTTAAAATCAATATTGAAAATAAAATAGAAAATAAGGATTTATTAATTAAACAATTAAAAGAAGAAAACAAAATACTCAAATTGGAAAACGCGATAGCAAAAAAAGTGAGTGCCTTGCTTCAATTGAAAGACTCACTAACAAAGAAAAATTCTAAATAA", "aa": "MQNNNIKILFKMNHKFRTFGKGSFLYNLNLENKKGRKSKINLDAQKATNFKINIENKIENKDLLIKQLKEENKILKLENAIAKKVSALLQLKDSLTKKNSK", "aa_hexdigest": "2fd688d2ae438f4492d977d31a37dcb3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XET5", "query_cov": 1.0, "subject_cov": 0.9901960784313726, "identity": 1.0, "score": 185.0, "evalue": 3.87e-59, "valid": true, "product": "Transposase", "uniref50_id": "UniRef50_C4XET5" }, "pscc": { "uniref50_id": "UniRef50_C4XET5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET5" ], "product": "Transposase" }, "genes": [], "id": "OAIKOHHJFB_801", "locus": "GCA000209735_00782" }, { "type": "cds", "sequence": "contig_1", "start": 437378, "stop": 438067, "strand": "+", "frame": 2, "gene": null, "product": "IS256 family transposase", "db_xrefs": [ "GO:0004803", "SO:0001217", "UniRef:UniRef50_C4XFF0", "UniRef:UniRef90_C4XET6" ], "nt": "TTGAAAAAGTCAACTTTTTATAAGATATTAAAATCAAAAAATAAACCTGACAAAGATAAAAATTTGAAAAAGATTATATTTGACTTATTTGACTATAATAAAGGGCTATACGGTTATAGACGTATTACTTTTGCTTTAAGAAATAAAGGAATAATAATCAATCATAAAAAAGTGTCGCTTATAAATGCGAGATATACTATTAAGGATTTTTTATTTATGAAAAGAAAACCAATAAATCCTGTTATTGATGAAATAACAGAAAAAATTTTAGAAAATTATAATCCAGTTACTTCTGGTGATCTTAGTATGGCTATGAAAGAAGTATTTCAAAATACAATCCAAAAAATGATGAACAAAGAATTTGATAATTTTATGGGTTATGAAAAAAATGATAATAAAGTTCAAAAAGAAAATTATAGAAACGGTTTTTCTAAGAAAAATGTTAATAGTCAATATGGTCAAATGGAAATTGACATTCCAAGAGATCGTGAAGCGAAATTTGAACCTATAATCATCAAAAAGTATGAACGTGATATTTCTGAATTAGTTGATATGGTATTTGCTTTATATTCTAGAGGAATGTCAACAAGAGATGTAAGTGATTTTATGTTTAGCAAATATGGCGTTAATTATTCTCCAACACAAATAAGCCAATTAACTAATGAAATTGTTGAAGATGCAAGATTATGA", "aa": "MKKSTFYKILKSKNKPDKDKNLKKIIFDLFDYNKGLYGYRRITFALRNKGIIINHKKVSLINARYTIKDFLFMKRKPINPVIDEITEKILENYNPVTSGDLSMAMKEVFQNTIQKMMNKEFDNFMGYEKNDNKVQKENYRNGFSKKNVNSQYGQMEIDIPRDREAKFEPIIIKKYERDISELVDMVFALYSRGMSTRDVSDFMFSKYGVNYSPTQISQLTNEIVEDARL", "aa_hexdigest": "6c839917bf40975022c366e9fab0935c", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 437309, "stop": 438319, "observations": { "mutation": [ 438065, 438245 ], "stop": [ 438065, 438245 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XET6", "query_cov": 1.0, "subject_cov": 0.6815476190476191, "identity": 0.996, "score": 446.0, "evalue": 7.69e-157, "gene_start": 1, "gene_end": 229, "reference_start": 24, "reference_end": 252, "reference_sequence": "MTIELKQEFKKLFSIKSILEKIKLKKSTFYKILKSKNKPDKDKNLKKIIFDLFDYNKGLYGYRRITFALRNKGIIINHKKVSLINARYTIKDFLFMKRKPINPVIDEITEKILENYNPVTSGDLSMAMKEVFQNTIQKMMNKEFDNFMGYEKNDNKVQKENYRNGFSKKNVNSQYGQMEIDIPRDREAKFEPIIIKKYERDISELVDMVFALYSRGMSTRDVSDFMFSKYGVNYSPTQISQLTNEIVEDARLWQERKLETYYPIIYIDAVHFHIVDNNVVTKKATYVIMGINGDGQKEILGLYIRENESAKFWMSVLNALKNRGISKIDIICSQII" }, "paralog": false, "identity": 0.9910714285714286, "score": 656.0, "evalue": 6.8e-242, "description": "Internal stop codon at 438065, 438245. Nonsense mutation around 438065, 438245.", "psc": { "uniref90_id": "UniRef90_C4XET6", "product": "IS256 family transposase", "uniref50_id": "UniRef50_C4XFF0", "go_ids": [ "GO:0004803" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XET6", "UniRef:UniRef50_C4XFF0", "GO:0004803" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFF0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFF0" ], "product": "Mutator family transposase" } }, "id": "OAIKOHHJFB_802", "locus": "GCA000209735_00783" }, { "type": "cds", "sequence": "contig_1", "start": 438301, "stop": 438516, "strand": "+", "frame": 1, "gene": null, "product": "Mutator family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET7", "UniRef:UniRef90_C4XET7" ], "nt": "TTGCTCACAGATAATTTAAAAGGTATACAACAAGCTATAGAAGCTGTTTTTCCTAATAGTAAGCAACAACGTTGCATTGTACATATGATTAGAAATTCTGTTAAATATGTTAACTACAAGGATATGAAAGAATTTTGCAAAGATTTAAAATCTGTATATCAATCTAATGATGCAAAAAATGCAATGGATAATTTGGATATTTTTGAAGATAAATGA", "aa": "MLTDNLKGIQQAIEAVFPNSKQQRCIVHMIRNSVKYVNYKDMKEFCKDLKSVYQSNDAKNAMDNLDIFEDK", "aa_hexdigest": "6adb078ae440bcf8fb6cce689e7cc88c", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 438301, "stop": 438819, "observations": { "mutation": [ 438514, 438547, 438559, 438742, 438766 ], "stop": [ 438514, 438547, 438559, 438742, 438766 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XET7", "query_cov": 1.0, "subject_cov": 0.4127906976744186, "identity": 1.0, "score": 144.0, "evalue": 1.38e-42, "gene_start": 1, "gene_end": 71, "reference_start": 1, "reference_end": 71, "reference_sequence": "MLTDNLKGIQQAIEAVFPNSKQQRCIVHMIRNSVKYVNYKDMKEFCKDLKSVYQSNDAKNAMDNLDIFEDKWGKKYPTSISIWRRNWSEISTMFDYSLEIRTLIYTTNSIENLNSVFRKYTKTKTVFPSDDSLLKILFLASQQIIKKWSNSRIRNWSSILNEFKIIDFENEE" }, "paralog": false, "identity": 0.9651162790697675, "score": 320.0, "evalue": 2.33e-114, "description": "Internal stop codon at 438514, 438547, 438559, 438742, 438766. Nonsense mutation around 438514, 438547, 438559, 438742, 438766.", "psc": { "uniref90_id": "UniRef90_C4XET7", "product": "Mutator family transposase", "uniref50_id": "UniRef50_C4XET7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XET7", "UniRef:UniRef50_C4XET7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XET7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET7" ], "product": "Mutator family transposase" } }, "id": "OAIKOHHJFB_803", "locus": "GCA000209735_00784" }, { "type": "cds", "sequence": "contig_1", "start": 439227, "stop": 439322, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCATTTTCTAATACATTATTCTCATATTTAAGAACAGTAGATCATGCTTCAACTACTTTCTTATTAAAACATAATGGTGAACCTATTTCATGA", "aa": "MPFSNTLFSYLRTVDHASTTFLLKHNGEPIS", "aa_hexdigest": "926f94348d4dc2b9400c4f72fc0640c7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3524.9525000000003, "isoelectric_point": 6.694576072692871 }, "id": "OAIKOHHJFB_804", "locus": "GCA000209735_00785" }, { "type": "cds", "sequence": "contig_1", "start": 439539, "stop": 439703, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTCAAATATCAAAAAAACATCAGATTCTAAAATAATGTTTAAAAATGATTTTCAAAGCAATTTTGTTAATTTAAATGATGCTCTTTACAAAATAAAATATGCCTTCGATTTCATTAAATACAATAATCCAACAAGCAATGGATATTATGAAGGAATATGA", "aa": "MLSNIKKTSDSKIMFKNDFQSNFVNLNDALYKIKYAFDFIKYNNPTSNGYYEGI", "aa_hexdigest": "dbccbc6003fce992d206baffb5cabe3e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6355.1217000000015, "isoelectric_point": 8.963700294494632 }, "id": "OAIKOHHJFB_805", "locus": "GCA000209735_00786" }, { "type": "cds", "sequence": "contig_1", "start": 440184, "stop": 440402, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCAATTAAAAATGATCAAATTTCTAAATTAGATAGTGAAGGTTTAAAAGCTTTGCAACAACTTGATGGTGGCGTTGATTTTGCAAAAATTAATTCAATTAAAAATTATTTAGAAAATGATTATTTATTAGCCGTTCAAGATTTAAGAGAATATTTCTTAAACAAACTAATTTACTTATTACATGAAAAATTCAATTATGGTGATGTATACTGA", "aa": "MNAIKNDQISKLDSEGLKALQQLDGGVDFAKINSIKNYLENDYLLAVQDLREYFLNKLIYLLHEKFNYGDVY", "aa_hexdigest": "be262ada710dde32b74c0c884593f0b0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8388.426699999996, "isoelectric_point": 4.867032051086425 }, "id": "OAIKOHHJFB_806", "locus": "GCA000209735_00787" }, { "type": "cds", "sequence": "contig_1", "start": 440514, "stop": 440681, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGCAAAATTAGCAGCAATAGCAAATGTAGATTCTAAAGTATATGAATATAAAAACAAAATTTTTGATGCTGCAATTGAAATTTATAAAAATGTTAATTCTACAGAAAACTTTAGTGTTATTCAAGCTGATAAAGACAACAAAGTTAACTTTGTTAAAAAATGA", "aa": "MDAKLAAIANVDSKVYEYKNKIFDAAIEIYKNVNSTENFSVIQADKDNKVNFVKK", "aa_hexdigest": "cde530d978da5e11e1277961cd9f5198", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6257.042100000002, "isoelectric_point": 7.942263221740723 }, "id": "OAIKOHHJFB_807", "locus": "GCA000209735_00788" }, { "type": "cds", "sequence": "contig_1", "start": 441504, "stop": 442043, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8", "UniRef:UniRef90_C4XFE8" ], "nt": "ATGTCAAACATAGATAAAATAGAAGAATTATCAGCAAAAATAGAAGATGCTAATACTATTGCAAGTAATAATTCTCCAATTTTAAATCCTATTAATGCTGTTAAAAAGATTTTATTATCAAGATTTGACTCTAATAATTATGACGTTATTTTAGAAAATGGTGGTGTTTTAGTAAATAGAAGTGTTATTATAAAAGATATTATTAAGGCATGTAAAACATTAGAACAATCATATGGAAATACAAAGGAATATATAAATCGTTATGTTAACACTTTTGTCAAAACAATTGAAAGTACAAAATTATTTAACAAATCTCAAATTTTAGACTTAAGCAGCAAATCATTAACGCCTATTGTTAATGCTCTTAATGAATTTAACTCATTTATTGAAAGTTCAAATCAAATTTCTTTTGAAAAATTAGATGAATTTATTAACAAAATTCAATTTATTCTAAACGCTGAAAATGATTTGACAACTTCTATTCAGTATGTTATTAAAGAATTTTTAAATGCTTATATTTCAAATGTTGATTCAATTTAA", "aa": "MSNIDKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILENGGVLVNRSVIIKDIIKACKTLEQSYGNTKEYINRYVNTFVKTIESTKLFNKSQILDLSSKSLTPIVNALNEFNSFIESSNQISFEKLDEFINKIQFILNAENDLTTSIQYVIKEFLNAYISNVDSI", "aa_hexdigest": "c60772a4f62025db3c02eb512f82dd76", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 441213, "stop": 442043, "observations": { "mutation": [ 441413, 441506 ], "stop": [ 441413, 441506 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE8", "query_cov": 1.0, "subject_cov": 0.6485507246376812, "identity": 0.922, "score": 314.0, "evalue": 2.02e-106, "gene_start": 1, "gene_end": 179, "reference_start": 1, "reference_end": 179, "reference_sequence": "MSNIDKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILEKNGVSVNRSVIIEDIIKACKTLEQSYGNTKEYINRYVNTFVKTIENTKLFNKSQILDLSNKSLTPIINGLNEFNSFVESSNQISFEKLDEFINKIQAILNVENDLTTSIQSVIKEFLNAYNSNADSIETTVKLAKLNNSKQIIDEFVQKSKIELSSLKWDNYIEGRNKLINEYVNSKNELDSIKNGFKKAFVNIKSRLNEFNLTLEANKTTLNEFYLFETSYKS" }, "paralog": false, "identity": 0.8804347826086957, "score": 464.0, "evalue": 7.02e-168, "description": "Internal stop codon at 441413, 441506. Nonsense mutation around 441413, 441506.", "psc": { "uniref90_id": "UniRef90_C4XFE8", "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE8", "UniRef:UniRef50_C4XFE8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 20239.749000000007, "isoelectric_point": 4.97110424041748 }, "id": "OAIKOHHJFB_808", "locus": "GCA000209735_00789" }, { "type": "cds", "sequence": "contig_1", "start": 442098, "stop": 442562, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7", "UniRef:UniRef90_C4XFE7" ], "nt": "TTGTGTCAAACTCAAATTGACTCAAATGAAAAACTTATTAAAGATTTACAAGGAATTATTGATCAACACTCTTCATATCATCTTGGTCACCACTTTGGTAACAAAAGTTATATGAGAACTGTTAAACATAGTAATGAAAAGAAAATAATTCAAAATCTTTTACCAGAAATGGTAGAAAGAACAAAACAAGTTATTAATCAAAGCGAATATGAAAATATCAAAAAATCAATCCAAGATTTTGTTGTTCGTAGTAATGATTTATTAACTTTAAAAACTGCAGATGAAAAATTTGTTGATGCTAATAGTAAAAGTGAATTAAATTCTTTAGAACTTAAAATTAAAGATTATCAAAACACTTTAAGTAAAATCGAAGAAGAAGCACTTTCAAGTAACACAAAACCTAACATTTCAACTGAAATGATTAATAAGGCAGCAGATTTTATTAAATCTGATTTTCATGTTTGA", "aa": "MCQTQIDSNEKLIKDLQGIIDQHSSYHLGHHFGNKSYMRTVKHSNEKKIIQNLLPEMVERTKQVINQSEYENIKKSIQDFVVRSNDLLTLKTADEKFVDANSKSELNSLELKIKDYQNTLSKIEEEALSSNTKPNISTEMINKAADFIKSDFHV", "aa_hexdigest": "17efb620cb5c875ee72ec80fcc4d0074", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 442062, "stop": 442823, "observations": { "mutation": [ 442100, 442577, 442592, 442616, 442694 ], "stop": [ 442100, 442577, 442592, 442616, 442694 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE7", "query_cov": 1.0, "subject_cov": 0.6039215686274509, "identity": 0.968, "score": 280.0, "evalue": 1.78e-93, "gene_start": 1, "gene_end": 154, "reference_start": 88, "reference_end": 241, "reference_sequence": "MKNSTKKIVALTMDISAGIVGAVSAGTLIGYAVDHSNYKKEKLWIENNIKYLKESNQKLKEKDNKLKEIWKKYENQEWLSSLWNELSLCQTQIDSNEKLIKDLQGIIDQHSSYHVGRRFGNKSYMRAVKHSNEKKIIQNLLPEMVERTKQVINQSEYENIKKSIQDFVVRSNDLLTLKTADEKFVDANSKSELNSLELKIKDYQNTLSKIEEEALSSNTKPNISTEMINKAADFIKSDFHVWNDKIIKKYVKKTA" }, "paralog": false, "identity": 0.9209486166007905, "score": 441.0, "evalue": 1.71e-159, "description": "Internal stop codon at 442100, 442577, 442592, 442616, 442694. Nonsense mutation around 442100, 442577, 442592, 442616, 442694.", "psc": { "uniref90_id": "UniRef90_C4XFE7", "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE7", "UniRef:UniRef50_C4XFE7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17770.864899999997, "isoelectric_point": 6.302102851867676 }, "id": "OAIKOHHJFB_809", "locus": "GCA000209735_00790" }, { "type": "cds", "sequence": "contig_1", "start": 442692, "stop": 442823, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAGCACAAAAAAAATTGTAGCTTTAACAATGGGTATTTCTGCAGGGATTGTTGGTGCAGTATCAGCCGGTACTTTAATTGGTTATGCAGTTGACCATTCAAACTATAAAAAAGAAAAGTTGTGA", "aa": "MKNSTKKIVALTMGISAGIVGAVSAGTLIGYAVDHSNYKKEKL", "aa_hexdigest": "28888bcde023630159e6cd6262b36ece", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4466.227499999999, "isoelectric_point": 9.778580665588379 }, "id": "OAIKOHHJFB_810", "locus": "GCA000209735_00791" }, { "type": "cds", "sequence": "contig_1", "start": 443064, "stop": 443492, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATTAACAACTATTAAATGCATCTCTAATTTTGATGAAATCCAAAAGTATATAGGATTGTTGTTTTTCAATGTTTTAATAATAAAACTTCAATTTTTTTCTAATTTATCAATGTTTTTAATAAATTCTTTATATTATCCAACTCTAATACCAGTTGAAATAAACACACGAAAAATGATACTAAATTTAGTGTTTTTGTGGCTTTTTAATTCTTCAACAAGCTTAATGAATTCCTTTTCAAGTATAGCTTGTCTTTTGTCTTTTTCTTCTTTTATATATAGAAGTTCATTTTTAACATCTGTATTTGTATTAGTGATTTTGCAAAATCAATTTAAAAAAGAAGAAAATATGTTTCTTTTAGTTAATTTAGTATTATTTGAAATTTTATTATTTGCTAATTCACGATTCATCCTTATAATTAATTGA", "aa": "MKLTTIKCISNFDEIQKYIGLLFFNVLIIKLQFFSNLSMFLINSLYYPTLIPVEINTRKMILNLVFLWLFNSSTSLMNSFSSIACLLSFSSFIYRSSFLTSVFVLVILQNQFKKEENMFLLVNLVLFEILLFANSRFILIIN", "aa_hexdigest": "19400a15aad0ab8224e517766e270f2b", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16629.7832, "isoelectric_point": 9.263607215881347 }, "id": "OAIKOHHJFB_811", "locus": "GCA000209735_00792" }, { "type": "cds", "sequence": "contig_1", "start": 443757, "stop": 444434, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTCAAGTAGTTGAAGTAACTAAAGAAATACCAACAACTCAATTAGAAATTCTTGAAGCAACAAACCAAGAAATTAAAGAAGCAAATGAAGTTATTGAAGAAGAAAATCAAGTTAAAGCAATAATAATTAATGAAAATAGTCAAGTAATTAACGTTAATGAAGAAGACTTTATCAGTCAAGAACACTCAGATACTATTATAGTTGAAGAAATTAAAGAAGTACCAGTTTTAGATAATGAAAAGAAAGATGAAACTATTGAAGAAGTTTTAGAAAAAATTAGCCAAGATCGTGAAAACACCAAAACTCAAGAAATTGAAAAATCTAAAGAAAATACAAAAGTTGTTGAAGCTATCAAAAAAGAAGAAACAGCTCAAGACACAATTACTTTGAATGCAGAAAAAACTCAAGAAGTTGGTTTTATTAAAGATGAAGCAACTATGGTTATTGAGCTTAATGTTGAAGAACAAAATGAACAAATTCAAAGTGTTTCACATTCGACTGAAAACACTATAAAACAGGGAGAAACAAAAAATATTGAAGCTGAAAATGCAATTGAAGAACAAGAAAAAACACTTGATCAATTAATCGAAGAAGGCGATAAAATTAAACAAAGTGATTATCTTGAAAGAGAAGAAGTTCCAGTTAAAGATTTTGAAGAATCAACAAAGGAATGA", "aa": "MTQVVEVTKEIPTTQLEILEATNQEIKEANEVIEEENQVKAIIINENSQVINVNEEDFISQEHSDTIIVEEIKEVPVLDNEKKDETIEEVLEKISQDRENTKTQEIEKSKENTKVVEAIKKEETAQDTITLNAEKTQEVGFIKDEATMVIELNVEEQNEQIQSVSHSTENTIKQGETKNIEAENAIEEQEKTLDQLIEEGDKIKQSDYLEREEVPVKDFEESTKE", "aa_hexdigest": "7a300d8eea78da7a586e90152292cf34", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25872.054900000025, "isoelectric_point": 4.257149696350097 }, "id": "OAIKOHHJFB_812", "locus": "GCA000209735_00793" }, { "type": "cds", "sequence": "contig_1", "start": 444489, "stop": 444788, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAACCATTGAAACTGAACCAGTTGAAGTTGAGAATTCATTATTAAATCAAGAAATCAAAAGCACAATTCTTGAAGCAATTAAAGATAAACCTTATCTAAAATTAATTCCAAATGAAACACTTGGAACACTTAAAGTTGATTATGTTTTAAGAAGAAAACATGAACCAATAATGACATTTATGATCGATGATTACAAATATGTTAATAATCCTGAAGACTTTGTTATTTTTAAAGACTTGTGTAAATTTATTAGATCTAAAAAATATATTGTTTATAAACTAGATCGAATTTTATGA", "aa": "MQTIETEPVEVENSLLNQEIKSTILEAIKDKPYLKLIPNETLGTLKVDYVLRRKHEPIMTFMIDDYKYVNNPEDFVIFKDLCKFIRSKKYIVYKLDRIL", "aa_hexdigest": "4c678a3d073495c3420da6f6874b85dc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11841.784199999986, "isoelectric_point": 6.582262229919434 }, "id": "OAIKOHHJFB_813", "locus": "GCA000209735_00794" }, { "type": "cds", "sequence": "contig_1", "start": 444867, "stop": 445199, "strand": "-", "frame": 3, "gene": null, "product": "AAA domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00313CCABD" ], "nt": "TTGTTGTGCTTCAATGCTAAACAACAAGATTCAATTACCACAAGCATTTTCAAAAATCATCCTGAATTAGAAAAAGCAATTAATAATGGAAACTTGTTATTAAGAAACATTGAAAATATTCAAGGCGATGAAGCTGATTTAGTTATAGCTTCAGTAGCTTATGATAAAGATACAGCAATTCACTCAACTTATGTTGGACGTCCTGGCGGTATGAATGCGTTAAATGTGGCAGTTAGTCGTGCTAAGGATAAAATGATTGTTATTAAATCACTTAAATCTAGTGACTTAACTATAATGACAGGTAATGAAGATGCTATTATGTTCAAACATTGA", "aa": "MLCFNAKQQDSITTSIFKNHPELEKAINNGNLLLRNIENIQGDEADLVIASVAYDKDTAIHSTYVGRPGGMNALNVAVSRAKDKMIVIKSLKSSDLTIMTGNEDAIMFKH", "aa_hexdigest": "8fbb0e89d4f835c318bccc7213e428da", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00313CCABD", "query_cov": 0.990909090909091, "subject_cov": 0.8384615384615385, "identity": 0.55, "score": 114.0, "evalue": 1.37e-30, "valid": false, "product": "AAA domain-containing protein", "uniref50_id": "UniRef50_UPI00313CCABD" }, "pscc": { "uniref50_id": "UniRef50_UPI00313CCABD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00313CCABD" ], "product": "AAA domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_814", "locus": "GCA000209735_00795" }, { "type": "cds", "sequence": "contig_1", "start": 445281, "stop": 445433, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTTTAGACAAAAACTATCGTTCAAATTATGCTTCACTGATGACTTTTAGCTCTAAATACTTCTACAAAAGTACTTTGGATGTTATCGATTCAGCTAATATGGAAGCTAATTTCAAGCCAATTGAAGTACATGAAGTTAATGGCCTTTGA", "aa": "MLLDKNYRSNYASLMTFSSKYFYKSTLDVIDSANMEANFKPIEVHEVNGL", "aa_hexdigest": "f812a67849f10f5f88119ebd0c2cab77", "start_type": "GTG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5796.5169, "isoelectric_point": 5.564048576354979 }, "id": "OAIKOHHJFB_815", "locus": "GCA000209735_00796" }, { "type": "cds", "sequence": "contig_1", "start": 445656, "stop": 446006, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAACACATTTAAAGAATTTCAAGGAAATTTAAATGATTCAGTTCAAGTTTTAAAAAACTTAAAGCAAGAAGACTTTGAATTTTTAAATGATTTAATAAACATTTTGCCTTCTAATAATACTCAAGAAACTAGCGATGAAAATGAATTAAAGAAATTTATTGCTCAAGGTATTATTAAACGAATTAACAAATTTACTGATGAAGAAAAAGATCAATACTCAGAATTTGCAGCCACAATTAGATTAGGAACATTAGAACCTTACAAGTTTATTAAGCGTTTTCCTAATATGATTAAAAAGATTTTCCCTATTGTAATTGTGACACCTGAAGCTGACTTAAGTGCCTGA", "aa": "MFNTFKEFQGNLNDSVQVLKNLKQEDFEFLNDLINILPSNNTQETSDENELKKFIAQGIIKRINKFTDEEKDQYSEFAATIRLGTLEPYKFIKRFPNMIKKIFPIVIVTPEADLSA", "aa_hexdigest": "0e7b14cd6ddc9d0f5dd14f3f8bc4719b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13523.284199999978, "isoelectric_point": 5.02566967010498 }, "id": "OAIKOHHJFB_816", "locus": "GCA000209735_00797" }, { "type": "cds", "sequence": "contig_1", "start": 446187, "stop": 446513, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTAACCTTTTAGTTTATGGCAAAAATTCTTTAGTTTGTTCTCAAAAGAAAGCGGCTCTTGAAGTTATTAAAAATAGAATGGGCGCTTTAAAAATCTTTTGTCTTTTCATGCTTAATTCAAAAAACATGAACAAAAAGAATTTCTACTTGCCTCTTAGAGAATATTTAGATTATTTAGAAAACTATGATGATAATCCTAATTTAAAACCATTAAGAATCATTAACCAAAAAGATATTCGTTTTGTTAACAATATAGCTAATTTCAGTAGTGATCCACGTTTTGTTAATATCACTCAAGCTTTAGCTAAAGTTCATAATAATTGA", "aa": "MTNLLVYGKNSLVCSQKKAALEVIKNRMGALKIFCLFMLNSKNMNKKNFYLPLREYLDYLENYDDNPNLKPLRIINQKDIRFVNNIANFSSDPRFVNITQALAKVHNN", "aa_hexdigest": "04326da75199e2c7d39d167e4568af7c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12600.588499999976, "isoelectric_point": 9.696447944641115 }, "id": "OAIKOHHJFB_817", "locus": "GCA000209735_00798" }, { "type": "cds", "sequence": "contig_1", "start": 446556, "stop": 446810, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATTTGAACCAGGCGTAGTTTTAGGCTTATTCCAACCAAGTGGTGGCTATGCCAGAAATAGAATGCTTGAAATAATTAAGAATAAAGAATTGAACAAAATTTTAGATATAGAATTTAACAAAAACATCTATAAAAATAGGGTAAAACAAACAATTTACAACCCTAAAACATCTATTTTTAAAATTACACCTACCAACTATTCTCAAGATAAAGCTATAGCTTCAAGCTTAAATCAAAACACAATTATTTGA", "aa": "MEFEPGVVLGLFQPSGGYARNRMLEIIKNKELNKILDIEFNKNIYKNRVKQTIYNPKTSIFKITPTNYSQDKAIASSLNQNTII", "aa_hexdigest": "161ac7f1ea6832377a31b4414712c963", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9665.068599999991, "isoelectric_point": 9.646098136901855 }, "id": "OAIKOHHJFB_818", "locus": "GCA000209735_00799" }, { "type": "cds", "sequence": "contig_1", "start": 446928, "stop": 447134, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCACTTAGGTTTCTTATTTGTTAAATTAGTTATTGACGATAAAGCTGTTTATGGTCCTTTATTTTTAAAAGAAGTTTATTTAGAAATTAGAAATGCTAGACCATATTTAGTTTCAAATGGTGACATTAAAATTAATGAAAAATTGCTATTCTTATTAAGTAATGCAGGTTTTGATTTAAATATCTCGGAAAACTATGGTGATTGA", "aa": "MHLGFLFVKLVIDDKAVYGPLFLKEVYLEIRNARPYLVSNGDIKINEKLLFLLSNAGFDLNISENYGD", "aa_hexdigest": "267726fef962aee9d0c9b16e4333d5e5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7777.940599999999, "isoelectric_point": 5.184250450134276 }, "id": "OAIKOHHJFB_819", "locus": "GCA000209735_00800" }, { "type": "cds", "sequence": "contig_1", "start": 447189, "stop": 447566, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAACAATGAAGAAAAACAATATCAAAGTATTCTAGACAATTTACTAGATGTTAGTCCAAATGACAGTGCAGTATTTACTAGAGTAAATAACAAATTTGCTTTTGATTTTTATACTATTTTTGGACCTGAAAGTTTTAGCAAAATTTACAACAATAAAAACTTTGACATACCTATTCTTGAAGTTAATTTGATCAGAATTTGTAATTTACTTGAAGAAGCAAATACATATGAAGAAATTATTAAAATTCTTGAAGAAAACAATATTGAAGTAAGTGAAAAACGTAATAGTCAATTAAAGAAAAATCCTCTTGGAATTAAAAATAACATTATTAATGAATTAAAAGTTAATTTTCAAAAACAATCATTAAAATGA", "aa": "MSNNEEKQYQSILDNLLDVSPNDSAVFTRVNNKFAFDFYTIFGPESFSKIYNNKNFDIPILEVNLIRICNLLEEANTYEEIIKILEENNIEVSEKRNSQLKKNPLGIKNNIINELKVNFQKQSLK", "aa_hexdigest": "ce8210b51537f9379f31bdd9e1b7bf39", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14605.367199999982, "isoelectric_point": 5.0644338607788075 }, "id": "OAIKOHHJFB_820", "locus": "GCA000209735_00801" }, { "type": "cds", "sequence": "contig_1", "start": 447774, "stop": 447914, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAAGAAAGCGATGAAAAATTCATCGGTGGAGTAATACCATTTATCCAATTCTTTACACACATTGGTGTTGTGGTAGTTTCTATTGTTGGTATTATTATTGAAAGCGTTAAAGGTAAAGAAAAGAAAAATGTTTGA", "aa": "MTKESDEKFIGGVIPFIQFFTHIGVVVVSIVGIIIESVKGKEKKNV", "aa_hexdigest": "ece4e6e2520054c07d59d020cc718c29", "start_type": "ATG", "rbs_motif": "GGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5061.974999999999, "isoelectric_point": 8.121743202209473 }, "id": "OAIKOHHJFB_821", "locus": "GCA000209735_00802" }, { "type": "cds", "sequence": "contig_1", "start": 448047, "stop": 448244, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAAGTAAGTGAAAAAAGTGGAGATGCAGGTAAGTTTTTAATAGCAAACAGACTTGTAACAAATGGAATTTTTGCAGACTTTTCAAACTTCACAAGTAATTTTTCAATAATTAAAAGCGTAGTTGAATCAGACACAATGGTTTATATAGCTTGTGGAATCTCAGCATTGTATTTTATCATTTATATTTCATGA", "aa": "MAKVSEKSGDAGKFLIANRLVTNGIFADFSNFTSNFSIIKSVVESDTMVYIACGISALYFIIYIS", "aa_hexdigest": "b777bb95341859c8d374dde6e0ccc2be", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7080.114299999999, "isoelectric_point": 5.997616386413574 }, "id": "OAIKOHHJFB_822", "locus": "GCA000209735_00803" }, { "type": "cds", "sequence": "contig_1", "start": 448245, "stop": 448349, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGATAAAGGCAAAAAAATTAAATACGCTTCTGTCATTACTTTGTCAATTATTTTAGCAATATTTACTGTTTTATTAATTATTGTTGCTAAAGGAAGTTGA", "aa": "MSDKGKKIKYASVITLSIILAIFTVLLIIVAKGS", "aa_hexdigest": "fadfcd6737dfef9ae20a803efaf1bb28", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3635.4870999999994, "isoelectric_point": 10.000287437438963 }, "id": "OAIKOHHJFB_823", "locus": "GCA000209735_00804" }, { "type": "cds", "sequence": "contig_1", "start": 449064, "stop": 449234, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGTTTTAATTAAAAAAGGACATACTAACATTTCATATCGAGATGAAAATAAGTTTATTCAAGAAAAAATTTTAAATAAATTTAATCACAAATTAGAATACAGTTTATTAAAAGATTTTAGTTTTACACCAAAACTTATTAGCCAAAATGACAAAGAAATCACTTGA", "aa": "MKVLIKKGHTNISYRDENKFIQEKILNKFNHKLEYSLLKDFSFTPKLISQNDKEIT", "aa_hexdigest": "96cc984c8c4b9940453da2d23516638b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6727.7394, "isoelectric_point": 9.522963523864746 }, "id": "OAIKOHHJFB_824", "locus": "GCA000209735_00805" }, { "type": "cds", "sequence": "contig_1", "start": 449321, "stop": 450292, "strand": "+", "frame": 2, "gene": null, "product": "Elongation factor 4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZP8" ], "nt": "ATGGACAAAAGCAAAATTAGAAATTTTTCAATTATTGCTCACATAGATCATGGTAAAAGTACGCTTGCTGATAGAATTCTAGAAATTACTAATACAGTTAGTGACCATGATATGGATGATCAATTTTTGGACCAAATGGATTTAGAACGTGAAAGAGGAATCACAATTAAATTAAATGCTGTACAAATTAAGTATAAAGATTACGTATTCCATTTAATTGATACACCAGGTCACGTTGATTTTACTTATGAAGTTTCACGTTCATTAGCTGCTAGTGAAGGAGCATTATTATTAGTTGATGCTACTCAAGGAATTGAAGCTCAAACTTTAGCTAATGTTTATTTAGCTCTTGAAAATAATTTAACTATTATTCCTATTATTAATAAAATTGATTTACCTTCAGCTGATATTGAAGCCACAAAAAATGAAATAGAAGAAGTGATTGGTCTACCAACTGATAATGCAGTTTGCATTTCAGCTAAAACTGGTTTAAATTGTGAAGCAGTTTTAGCTGCAATTGAAAAATATATTCCAGCACCTAAAGATGCTGATGATTCTAAACCTCTTAAAGCTTTAATTTTTGACTCGTACTTCGATGAATATCGTGGTGTAGTTATGCTTATAAGAATTTTTGAAGGTGAATTAAAAGTTGGCGATGAATTTATGTTTATGTCGAATGGACAAAGATATTTTGTTAGTGAACTTGGAGTTAGAAGTCCAAAAGAAACTAAGAAAAAGAGACTTGAAGCAGGTGAAGTTGGATATGTAGCAGCTACTATTAGAGATGCTAGAGAAGTAAGCGTTGGAGATACTATAACTTTAGTTAATAGACCAGCTGAAAAAGCACTGCCAGGTTACAAAAAGAAAAAACCTGTGCTTTATACTGGGTTTTATCCAATTGATACTCGCGACTATATGGAACTTAAAGAAAGTTTAGAAAAAATAGCTCTTTCAGATAGCTCAATAACTTGA", "aa": "MDKSKIRNFSIIAHIDHGKSTLADRILEITNTVSDHDMDDQFLDQMDLERERGITIKLNAVQIKYKDYVFHLIDTPGHVDFTYEVSRSLAASEGALLLVDATQGIEAQTLANVYLALENNLTIIPIINKIDLPSADIEATKNEIEEVIGLPTDNAVCISAKTGLNCEAVLAAIEKYIPAPKDADDSKPLKALIFDSYFDEYRGVVMLIRIFEGELKVGDEFMFMSNGQRYFVSELGVRSPKETKKKRLEAGEVGYVAATIRDAREVSVGDTITLVNRPAEKALPGYKKKKPVLYTGFYPIDTRDYMELKESLEKIALSDSSIT", "aa_hexdigest": "3da9e17740625522f70716f9a6309cba", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A449BB04", "query_cov": 0.9256965944272446, "subject_cov": 0.9373040752351097, "identity": 0.833, "score": 494.0, "evalue": 1.66e-174, "valid": false, "product": "GTP-binding protein lepA", "uniref50_id": "UniRef50_A0A448ZZP8" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZP8" ], "product": "Elongation factor 4" }, "genes": [], "id": "OAIKOHHJFB_825", "locus": "GCA000209735_00806" }, { "type": "cds", "sequence": "contig_1", "start": 450347, "stop": 451120, "strand": "+", "frame": 2, "gene": "lepA", "product": "translation elongation factor 4", "db_xrefs": [ "EC:3.6.5.n1", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005886", "GO:0043022", "GO:0045727", "GO:1990606", "SO:0001217", "UniRef:UniRef50_Q8GCP5", "UniRef:UniRef90_Q8GCP5" ], "nt": "ATGTTACATATGGAGATATTGCAAGAGAGATTAAATCGTGAATATAAAATCGGTATTATAGCAACTAGTCCTTCAGTTGAATATGATGTTCATATGACAGATGGCAGCATTAAAAAAATATCAAACCCTGCACTTTTACCTGATCGAACTTTTATCGATCATATTGATGAACCTTACATTAAAGCTGAGATTTTGGTACCTAATGAATACATAGGAAATGTTATGGAACTATGTCAAAATAAACGTGGAATTTATATCAATATGGACTATATTGATGCTAATAGATCAAGGCTTATTTATGAAATGCCACTAGGCGAAATTATCTTTGATTTCTTTGATAGACTTAAGAGTCTAACTAAAGGTTATGCATCATTTGAATATGACCTAATAGGCTATAAAACAAGTGATTTGGTAAAAGTAGACATACTTTTAAATGGTGATAAAATTGATGCTTTTTCAATTATTACTCATAAAGATTCAGCTTATAGTAAAGCTCGTGATTTAACTCAAAAATTAAAAGAAGCTATACCAAGACAAAACTTTGAAGTACCTGTTCAAGCAACTATTGGAAGTAAAATTATTGCTAGAGAAACTATTAAAGCTTATCGTAAAGATGTTACTTCAAAACTTCATGCAGCTGATGTTTCAAGATATAAAAAACTTTTAGAAAAGCAAAAAGCGGGTAAGAAAAAAATGAAAATGCTTGGTACTGTTGAAGTTCCTCAAGAAGCATTTTTATCAATTCTTAAAACTAATATTGATAAGAAAAAATAG", "aa": "MLHMEILQERLNREYKIGIIATSPSVEYDVHMTDGSIKKISNPALLPDRTFIDHIDEPYIKAEILVPNEYIGNVMELCQNKRGIYINMDYIDANRSRLIYEMPLGEIIFDFFDRLKSLTKGYASFEYDLIGYKTSDLVKVDILLNGDKIDAFSIITHKDSAYSKARDLTQKLKEAIPRQNFEVPVQATIGSKIIARETIKAYRKDVTSKLHAADVSRYKKLLEKQKAGKKKMKMLGTVEVPQEAFLSILKTNIDKKK", "aa_hexdigest": "fc4c3be7f24ef9cedbc56b387e762b6f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A449BBE2", "query_cov": 1.0, "subject_cov": 0.8741496598639455, "identity": 0.767, "score": 399.0, "evalue": 4.02e-138, "valid": false, "product": "GTP-binding protein lepA", "uniref50_id": "UniRef50_A0A449BBE2" }, "genes": [ "lepA" ], "truncated": "5-prime", "pseudogene": { "start": 450047, "stop": 451120, "observations": { "mutation": [ 450290 ], "stop": [ 450290 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q8GCP5", "query_cov": 1.0, "subject_cov": 0.4290484140233723, "identity": 1.0, "score": 501.0, "evalue": 7.02e-174, "gene_start": 1, "gene_end": 257, "reference_start": 343, "reference_end": 599, "reference_sequence": "MDKSKIRNFSIIAHIDHGKSTLADRILEITNTVSDHDMDDQFLDQMDLERERGITIKLNAVQIKYKDYVFHLIDTPGHVDFTYEVSRSLAASEGALLLVDATQGIEAQTLANVYLALENNLTIIPIINKIDLPSADIEATKNEIEEVIGLPTDNAVCISAKTGLNCEAVLAAIEKYIPAPKDADDSKPLKALIFDSYFDEYRGVVMLIRIFEGELKVGDEFMFMSNGQRYFVSELGVRSPKETKKKRLEAGEVGYVAATIRDAREVSVGDTITLVNRPAEKALPGYKKKKPVLYTGFYPIDTRDYMELKESLEKIALSDSSITWEQETSKALGFGFRVGFLGMLHMEILQERLNREYKIGIIATSPSVEYDVHMTDGSIKKISNPALLPDRTFIDHIDEPYIKAEILVPNEYIGNVMELCQNKRGIYINMDYIDANRSRLIYEMPLGEIIFDFFDRLKSLTKGYASFEYDLIGYKTSDLVKVDILLNGDKIDAFSIITHKDSAYSKARDLTQKLKEAIPRQNFEVPVQATIGSKIIARETIKAYRKDVTSKLHAADVSRYKKLLEKQKAGKKKMKMLGTVEVPQEAFLSILKTNIDKKK" }, "paralog": false, "identity": 0.9971988795518207, "score": 695.0, "evalue": 9.25e-253, "description": "Internal stop codon at 450290. Nonsense mutation around 450290.", "psc": { "uniref90_id": "UniRef90_Q8GCP5", "gene": "lepA", "product": "translation elongation factor 4", "ec_ids": [ "3.6.5.n1" ], "uniref50_id": "UniRef50_Q8GCP5", "go_ids": [ "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005886", "GO:0043022", "GO:0045727", "GO:1990606" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q8GCP5", "EC:3.6.5.n1", "UniRef:UniRef50_Q8GCP5", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005886", "GO:0043022", "GO:0045727", "GO:1990606" ] }, "pscc": { "uniref50_id": "UniRef50_Q8GCP5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8GCP5" ], "product": "Elongation factor 4" } }, "id": "OAIKOHHJFB_826", "locus": "GCA000209735_00807" }, { "type": "cds", "sequence": "contig_1", "start": 451405, "stop": 451875, "strand": "+", "frame": 1, "gene": null, "product": "MAGa4850 family ICE element protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF94", "UniRef:UniRef90_C4XF94" ], "nt": "ATGAGTGTATACGGCGAACTAAGGTTAATTGCAAATGAAGGAAGCGAAAACGAAGTTAGAAAGTTTGAAGCTAACTTGGAATGGAGAAAAAATTATTTTGGCAAGAAGGTTTATGACAAGTTAAGCCAAGATACAGTTAGTGAAATTTTAAAAACAAAGATAGTACTCGGTGAAAGCTACTATAAGATTTTGAGAACTGAAAAAGTTGCTTTTGAAGTGTATGTCTGTTTAAGGTTTCTTGGGGCTAAAAAACGCTATGTAAATTTTTATGAATTAGTGGCTTTTGGCTTCAAGAAAACAAGCTTGCAAAGAGCGGTAAAGTTTTTAACAGATATAGGGCTAATTTTAAAAGTTAGAAACGCTGTGAAAATTAAGAAGTTTAAGCTAACTAATGACGATAGAAAGTTCATTGTTATTAGTGGTGATAAAGACTGGAAGATTTTCTTGTTGTTTGGATTAGCTAATTTATGA", "aa": "MSVYGELRLIANEGSENEVRKFEANLEWRKNYFGKKVYDKLSQDTVSEILKTKIVLGESYYKILRTEKVAFEVYVCLRFLGAKKRYVNFYELVAFGFKKTSLQRAVKFLTDIGLILKVRNAVKIKKFKLTNDDRKFIVISGDKDWKIFLLFGLANL", "aa_hexdigest": "64ceadfbbde65f76d363fcfbc070e5a5", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 451333, "stop": 452124, "observations": { "mutation": [ 451873, 451894 ], "stop": [ 451873, 451894 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF94", "query_cov": 1.0, "subject_cov": 0.5931558935361216, "identity": 0.9940000000000001, "score": 300.0, "evalue": 2.18e-101, "gene_start": 1, "gene_end": 156, "reference_start": 25, "reference_end": 180, "reference_sequence": "MLPVRPVVYIIPQSLLYVQKKGEIMSVYGELRLIANEGSENEVRKFEANLEWRKNYFGKKVYDKLSQDTVSEILKTKIVLGESYYKILRTEKVAFEVYVCLRFLGAKKRYVNFYELVAFGFKKTSLQRAVKFLTDIGLILKVRNAVKIKKFKLTNDDRKFIVISGYKDWKIFLLFGLANLWAYKTLVWKSKELGTKKFVKSRKMKVIVQNNFLGLKGSTAYRYLKNICLVLGLRTDELFIIQRSVDNLQHLSFKTQRYLVIRI" }, "paralog": false, "identity": 0.9695817490494296, "score": 496.0, "evalue": 4.23e-181, "description": "Internal stop codon at 451873, 451894. Nonsense mutation around 451873, 451894.", "psc": { "uniref90_id": "UniRef90_C4XF94", "product": "MAGa4850 family ICE element protein", "uniref50_id": "UniRef50_C4XF94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF94", "UniRef:UniRef50_C4XF94" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF94" ] } }, "id": "OAIKOHHJFB_827", "locus": "GCA000209735_00808" }, { "type": "cds", "sequence": "contig_1", "start": 452636, "stop": 452809, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTAGCCAATATTCTGGTGTTTATGATATGTTAAACGATTCTAGTGCTGATGTAAATATCGATAACGTTAAGGAAGAATTAAAGAGTATTGATGAACAAACATTTTATGAAGGCATTTTAAACTTAGGTGATTTAGGTTTAGAACATAATGTTTTAACTGCTAATGATTGA", "aa": "MGSQYSGVYDMLNDSSADVNIDNVKEELKSIDEQTFYEGILNLGDLGLEHNVLTAND", "aa_hexdigest": "a6e4eac163e8f8773d728fed3a44313e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6295.746999999999, "isoelectric_point": 4.0500284194946286 }, "id": "OAIKOHHJFB_828", "locus": "GCA000209735_00809" }, { "type": "cds", "sequence": "contig_1", "start": 452927, "stop": 453067, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTTGAAAAGACTAAAAAAAGAGCAAAAAGAAAATTAAACATCGACGATATAAAAATGCTAAGAATGAAGATTACAAACAAGCTTATTAAAAACTTTGATTATCAAAATCTTAAGGATTTAACAAGTAAGTTATGA", "aa": "MFFEKTKKRAKRKLNIDDIKMLRMKITNKLIKNFDYQNLKDLTSKL", "aa_hexdigest": "3818316b54ed4b32cdd1dece0c67879e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5661.7964, "isoelectric_point": 10.291749000549316 }, "id": "OAIKOHHJFB_829", "locus": "GCA000209735_00810" }, { "type": "cds", "sequence": "contig_1", "start": 453173, "stop": 453514, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGAATAGGTTCAATAAGTCTTATAAACTCGCTTCACCAGAAGTTAAAGAAGCGATAAACAAGATAGAAACATTTTTATATAAATATGATGATGATTTTAAAGGAAAGATGGATAACTATCAAAAGGCATTTGATTTAGTTATGCAACTAAAGGATGAAACGAATTCAACGTTTTTAAAAAAGAGAATCAATAATGTTTTGAGAACTGAAAAAGATGAGTTTTCTAGACAGCTAGGGAACAAGATAATAAAAGCAATTCTAAAATCAAGAAATGCTAAGAGCGCTAAAAGCTTTACAAAATTATTCTTTTATAACTTGAATGTAAATGATGATTCTTGA", "aa": "MENRFNKSYKLASPEVKEAINKIETFLYKYDDDFKGKMDNYQKAFDLVMQLKDETNSTFLKKRINNVLRTEKDEFSRQLGNKIIKAILKSRNAKSAKSFTKLFFYNLNVNDDS", "aa_hexdigest": "ed4157fbcdf2d18665df0a32937d7513", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13380.135499999978, "isoelectric_point": 9.546301078796386 }, "id": "OAIKOHHJFB_830", "locus": "GCA000209735_00811" }, { "type": "gap", "sequence": "contig_1", "start": 453737, "stop": 453836, "strand": ".", "length": 100, "id": "OAIKOHHJFB_1802" }, { "type": "cds", "sequence": "contig_1", "start": 454022, "stop": 454228, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEU7", "UniRef:UniRef90_C4XEU7" ], "nt": "ATGTCTTATACAGGAAATTTTGATACTTTTTCAACATTCTTTCAAAACTATGGATTAAATTATGACAAGTTAGAATCAGAAAAAAAACAAATAATAATTAATAAAAAATTATTACCAGAGAAAACGGCAAATTCTAAAATTTCTGAAACTAAAACTGAATCTTTGTTCAAAAAGATTAGTTCAAAATCTTTATTTGAAAAAATGTGA", "aa": "MSYTGNFDTFSTFFQNYGLNYDKLESEKKQIIINKKLLPEKTANSKISETKTESLFKKISSKSLFEKM", "aa_hexdigest": "d14ed2add281c7a34f2d13d149b41d1e", "start_type": "ATG", "rbs_motif": "AGGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 453929, "stop": 454228, "observations": { "mutation": [ 453952, 453958, 454024 ], "stop": [ 453952, 453958, 454024 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEU7", "query_cov": 1.0, "subject_cov": 0.6666666666666666, "identity": 1.0, "score": 126.0, "evalue": 1.49e-36, "gene_start": 1, "gene_end": 68, "reference_start": 1, "reference_end": 68, "reference_sequence": "MSYTGNFDTFSTFFQNYGLNYDKLESEKKQIIINKKLLPEKTANSKISETKTESLFKKISSKSLFEKMWKSKKYLNFADKALIFLSTLFKMKIQYQKEFLMN" }, "paralog": false, "identity": 0.9191919191919192, "score": 173.0, "evalue": 2.02e-57, "description": "Internal stop codon at 453952, 453958, 454024. Nonsense mutation around 453952, 453958, 454024.", "psc": { "uniref90_id": "UniRef90_C4XEU7", "uniref50_id": "UniRef50_C4XEU7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEU7", "UniRef:UniRef50_C4XEU7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEU7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEU7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7940.015899999999, "isoelectric_point": 9.398668479919433 }, "id": "OAIKOHHJFB_831", "locus": "GCA000209735_00812" }, { "type": "cds", "sequence": "contig_1", "start": 454598, "stop": 454726, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTATGAGTTATACAATGTCATATTAACTTTAAGAAAAGAAATTAAAGAAAACTTTGAAAAAGATGTTCTAGTTATTGAAGAAGAACTTAAAAAAAGCGGTCATAAAAAATTAGCAGAAATATGA", "aa": "MIYELYNVILTLRKEIKENFEKDVLVIEEELKKSGHKKLAEI", "aa_hexdigest": "592dbaac8b237b3688e3fe44e99cd674", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5047.9042, "isoelectric_point": 5.739283180236817 }, "id": "OAIKOHHJFB_832", "locus": "GCA000209735_00813" }, { "type": "cds", "sequence": "contig_1", "start": 454883, "stop": 455305, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCTTTTGAATATGACAATAATGTTTTGTATGACAAATCATTAGATATGACATTATCGAAAAATCAAGAAAACATAGCTCAAATATGTGACATGATGGATATGATAATTGGCAGCAGTCAAACCGGAATAATAAATAGTGATAATGTTGAATTAAATACTTTAATGAAAGAAGTTGAATCAGCAGTTAATTTTAAAGACAAATTGAGCTTTTTTACAAGTCAAAGTGGAAAAGACAAATTTTACCAATTCTTCGATAAAATGCTTTATTTAGCTTTAGATGTTAACGCTCAAATATTCAATGATTCAAGATTATTAGATGGTTTAACAGAAGACGATGAAAATCAACAACAAAGAAGTAGATTCTATATTCTTAAGCATAAATATTTTGAAAAAAATAAACAAATTTTAAATGAAGACTGA", "aa": "MSFEYDNNVLYDKSLDMTLSKNQENIAQICDMMDMIIGSSQTGIINSDNVELNTLMKEVESAVNFKDKLSFFTSQSGKDKFYQFFDKMLYLALDVNAQIFNDSRLLDGLTEDDENQQQRSRFYILKHKYFEKNKQILNED", "aa_hexdigest": "d439b38e85e9fa7d10c06e131121e0cb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16448.27789999999, "isoelectric_point": 4.554019737243652 }, "id": "OAIKOHHJFB_833", "locus": "GCA000209735_00814" }, { "type": "cds", "sequence": "contig_1", "start": 456223, "stop": 456861, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01653.23" ], "nt": "ATGCTTTCTTTATCTAAAGCATATGACTATAGTGAAATTGAAAAATATGTCAATAATATTGAAAAATTAGTTCCAATTGAAGACATCAATTTTTCAATTGAGCCTAAAATTGATGGTCTTTCAATTTCACTTCATTATAATCATGGCTTCTTAAACCAAGCTTTAACAAGAGGTGATGGTCATGAGGGTGAAGATGTAACTGAAAATATTTATCAAATAGCTTCAATACCAAAAATAATTAATTATGATGCTGAATTAGAAGTTAGAGGTGAAGTATTTTTACCTAAAGCTGAGTTTCAAAGATTAAATGAAAATTTAATTAAAGCAGGCGAAAAACCTTTTTCAAATCCAAGAAATGCAGCAAGTGGGACTTTAAGACAATTAGATCCTACTATTGTTAAAAATAGACAACTTGCTTCCTTTTTATATGAATTAGTTGAGCCTCAAAAACATAATATTACCACTCAAGAACAAGCTTTAGAATTCTTTCAAAAATTAAATATACCCACTAATCCATATCATAAAGTAGTTGAAGTTGAAGAATTGGAAGAAGCAATTAGTTTTTTTGCTGAAATGAAAAATAAACTCAATTATGATGCTGATGGTTTAGTAATTAAGCTAAATAATTTAACCTATTGA", "aa": "MLSLSKAYDYSEIEKYVNNIEKLVPIEDINFSIEPKIDGLSISLHYNHGFLNQALTRGDGHEGEDVTENIYQIASIPKIINYDAELEVRGEVFLPKAEFQRLNENLIKAGEKPFSNPRNAASGTLRQLDPTIVKNRQLASFLYELVEPQKHNITTQEQALEFFQKLNIPTNPYHKVVEVEELEEAISFFAEMKNKLNYDADGLVIKLNNLTY", "aa_hexdigest": "62ccb3cb0c9a68c7c406150ba77312b8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01653.23", "name": "DNA_ligase_aden", "length": 218, "aa_cov": 0.9952830188679245, "hmm_cov": 1.0, "evalue": 1.0172512822207865e-71, "score": 237.5041961669922, "start": 1, "stop": 211 } ], "seq_stats": { "molecular_weight": 24290.117800000015, "isoelectric_point": 4.9036363601684565 }, "id": "OAIKOHHJFB_834", "locus": "GCA000209735_00815" }, { "type": "cds", "sequence": "contig_1", "start": 456868, "stop": 457965, "strand": "+", "frame": 1, "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "db_xrefs": [ "EC:6.5.1.2", "GO:0003677", "GO:0003911", "GO:0006260", "GO:0006281", "SO:0001217", "UniRef:UniRef50_A0A953T6S3", "UniRef:UniRef90_C4XEU9" ], "nt": "ATGGGTAAAACTGCTAAGTTTCCAAAACATTCAATTGCTTTTAAATATGAAGTTGAAACAGCAATTAGTACTATTAAAGATATTAAAACCACAGTGGGTCGTACTGGCAAAATTACTTATGTTGCAAGTTTAGAACCCGTTGAATTAAATCAAACAATAGTTAGAGCAGCAACTCTTCATAATTATAATTTTATTGAAGACTTAAACATTAACATTAATGATCAAGTTCAAATAGTTAAGGCTGGTGAAATAATTCCTAAAGTTTTAGCTTTAGTTAAAAAACAGTCTAAAGGTGTTTTTGGTAAAACTTTAGAATGTCCTTCTTGTGGCAGTGTATTAGTCGAAATTGAAGATAATGTTGATCAATTTTGTGTTAACAAAAATTGTCCTGAAATGACAATTAATGCAATTTATCACTTTGCTTCAAGAAAATCGTTAAATATTGTAGGTTTAGGATTAATCACAGTTAAAGATTTCTATAAACACAATTTAGTTAAAAGCATTGAAGATATTTTTAATTTACATAAACATAAAGATGAATTATTAAAACTTGAACGTTATGCAGAGTTAAAAGTTAACAATTTATTAACTAATATTGGAAAAGCAAAAGACTCGACTTTTAGTAAGGTTTTATTTGCTCTTGGTATTAAACATGTGGGTGAAAGAGCTGCAAAATTAATTTCAAAACAATATCATAATTTTGACGAATTAATTTGTGAAAAAGATGAATTGGCAAAAATATCAGCTATAAATAATATTGGTCCTAAAATTTTAGATAGCTTAAAAGAATATCTAAAAAATGAAGAAAATGTTAAACTTTTAAGAAAATTTGATGAGATATTTAATTATCAAAAAATTGCTACAGTTGCTAGTGAAAAATTAATTAATTTATCATTTGTTATCACAGGTAAATTATCAAACTCAAGGGATCACTATGTTGATTTAGTTGAAGCTAATGGAGGCAAAGTATCTTCATCAGTTTCTTCTAAAACGTCATTTCTTTTAGTAGGAGAAGATGCAGGTAGCAAATTAGATAAAGCTAAAAAATTAAATATTGCAATAATAAATGAAGAACAATTTAATGAAATGTTAAAATAG", "aa": "MGKTAKFPKHSIAFKYEVETAISTIKDIKTTVGRTGKITYVASLEPVELNQTIVRAATLHNYNFIEDLNININDQVQIVKAGEIIPKVLALVKKQSKGVFGKTLECPSCGSVLVEIEDNVDQFCVNKNCPEMTINAIYHFASRKSLNIVGLGLITVKDFYKHNLVKSIEDIFNLHKHKDELLKLERYAELKVNNLLTNIGKAKDSTFSKVLFALGIKHVGERAAKLISKQYHNFDELICEKDELAKISAINNIGPKILDSLKEYLKNEENVKLLRKFDEIFNYQKIATVASEKLINLSFVITGKLSNSRDHYVDLVEANGGKVSSSVSSKTSFLLVGEDAGSKLDKAKKLNIAIINEEQFNEMLK", "aa_hexdigest": "37df700e8a13e5c038b373723d1a13b1", "start_type": "ATG", "rbs_motif": null, "genes": [ "ligA" ], "truncated": "5-prime", "pseudogene": { "start": 456568, "stop": 457965, "observations": { "mutation": [ 456859 ], "stop": [ 456859 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEU9", "query_cov": 1.0, "subject_cov": 0.5496987951807228, "identity": 1.0, "score": 701.0, "evalue": 5.64e-250, "gene_start": 1, "gene_end": 365, "reference_start": 300, "reference_end": 664, "reference_sequence": "MRRAMNALDIKKKIIELSNKLNQWNKEYYQDNNPSVSDFEYDKALQELEQLEKKYPQFIYVNSPTQKLGSFADNKFKKVVHQKPMLSLSKAYDYSEIEKYVNNIEKLVPIEDINFSIEPKIDGLSISLHYNHGFLNQALTRGDGHEGEDVTENIYQIASIPKIINYDAELEVRGEVFLPKAEFQRLNENLIKAGEKPFSNPRNAASGTLRQLDPTIVKNRQLASFLYELVEPQKHNITTQEQALEFFQKLNIPTNPYHKVVEVEELEEAISFFAEMKNKLNYDADGLVIKLNNLTYWNKMGKTAKFPKHSIAFKYEVETAISTIKDIKTTVGRTGKITYVASLEPVELNQTIVRAATLHNYNFIEDLNININDQVQIVKAGEIIPKVLALVKKQSKGVFGKTLECPSCGSVLVEIEDNVDQFCVNKNCPEMTINAIYHFASRKSLNIVGLGLITVKDFYKHNLVKSIEDIFNLHKHKDELLKLERYAELKVNNLLTNIGKAKDSTFSKVLFALGIKHVGERAAKLISKQYHNFDELICEKDELAKISAINNIGPKILDSLKEYLKNEENVKLLRKFDEIFNYQKIATVASEKLINLSFVITGKLSNSRDHYVDLVEANGGKVSSSVSSKTSFLLVGEDAGSKLDKAKKLNIAIINEEQFNEMLK" }, "paralog": false, "identity": 0.9978494623655914, "score": 905.0, "evalue": 0.0, "description": "Internal stop codon at 456859. Nonsense mutation around 456859.", "psc": { "uniref90_id": "UniRef90_C4XEU9", "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "ec_ids": [ "6.5.1.2" ], "uniref50_id": "UniRef50_A0A953T6S3", "go_ids": [ "GO:0003677", "GO:0003911", "GO:0006260", "GO:0006281" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEU9", "EC:6.5.1.2", "UniRef:UniRef50_A0A953T6S3", "GO:0003677", "GO:0003911", "GO:0006260", "GO:0006281" ] }, "pscc": { "uniref50_id": "UniRef50_A0A953T6S3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A953T6S3" ], "product": "DNA ligase" } }, "id": "OAIKOHHJFB_835", "locus": "GCA000209735_00816" }, { "type": "cds", "sequence": "contig_1", "start": 458098, "stop": 458592, "strand": "+", "frame": 1, "gene": "tpiA", "product": "triose-phosphate isomerase", "db_xrefs": [ "EC:5.3.1.1", "GO:0004807", "GO:0006096", "KEGG:K01803", "SO:0001217", "UniRef:UniRef50_P50919", "UniRef:UniRef90_C4XEV0" ], "nt": "ATGAACAAAAATTTTACAGAAACCATGGACTTTATTTTATCTTTTAAAGATGAATACAAAAAATTAAAGAAAAACAAAGAAGCATATGCAATTAGTCAACTAAATGAATTTGCTGTTGCAATACCTCATGCTAATTTATCAGCTTATCAAATTAACAAAGTTAATGAATTAAAACTATGTGCTCAAGATGTTAGTCAAAATTGCAATGGTGCTTTTACAGGTGATGTTAGCGCTTCAATGCTACAAGATCTTAATGTTAACTATGTAATTCTAGGTCACTCAGAAAGAAGACAATATCATCACGAAACAAATGAGTTAGTTAACTTAAAAGCTAAACAAGCTCTTGAATACAATTTGACACCAGTTATTTGTGTTGGTGAAACATTAGAACAATATGAAAAAGGTCAAACAAAAGCAATTGTTAAAAAACAAATTGAAGAAAGTTTAAAAGATTTAGATTATTCAAAAATTATAGTTGCTTATGAACCAATTTGA", "aa": "MNKNFTETMDFILSFKDEYKKLKKNKEAYAISQLNEFAVAIPHANLSAYQINKVNELKLCAQDVSQNCNGAFTGDVSASMLQDLNVNYVILGHSERRQYHHETNELVNLKAKQALEYNLTPVICVGETLEQYEKGQTKAIVKKQIEESLKDLDYSKIIVAYEPI", "aa_hexdigest": "daac67b9ba546fb79c39dce817c0ffaf", "start_type": "ATG", "rbs_motif": null, "genes": [ "tpiA" ], "truncated": "both", "pseudogene": { "start": 458026, "stop": 458814, "observations": { "mutation": [ 458092, 458590 ], "stop": [ 458092, 458590 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEV0", "query_cov": 1.0, "subject_cov": 0.6259541984732825, "identity": 1.0, "score": 319.0, "evalue": 1.02e-108, "gene_start": 1, "gene_end": 164, "reference_start": 25, "reference_end": 188, "reference_sequence": "MYNLINYLGVIMDIRKYVVIGNWKMNKNFTETMDFILSFKDEYKKLKKNKEAYAISQLNEFAVAIPHANLSAYQINKVNELKLCAQDVSQNCNGAFTGDVSASMLQDLNVNYVILGHSERRQYHHETNELVNLKAKQALEYNLTPVICVGETLEQYEKGQTKAIVKKQIEESLKDLDYSKIIVAYEPIWAIGTGKVATPEIAQDVCQYIHSITSKELVVQYGGSVNPKNISDLAAQKDINGFLVGGASLNVDSFIKLITLGK" }, "paralog": false, "identity": 0.9885496183206107, "score": 509.0, "evalue": 3.69e-186, "description": "Internal stop codon at 458092, 458590. Nonsense mutation around 458092, 458590.", "psc": { "uniref90_id": "UniRef90_C4XEV0", "gene": "tpiA", "product": "triose-phosphate isomerase", "ec_ids": [ "5.3.1.1" ], "uniref50_id": "UniRef50_P50919", "kegg_orthology_id": "K01803", "go_ids": [ "GO:0004807", "GO:0006096" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEV0", "EC:5.3.1.1", "UniRef:UniRef50_P50919", "KEGG:K01803", "GO:0004807", "GO:0006096" ] }, "pscc": { "uniref50_id": "UniRef50_P50919", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P50919" ], "product": "Triosephosphate isomerase" } }, "id": "OAIKOHHJFB_836", "locus": "GCA000209735_00817" }, { "type": "cds", "sequence": "contig_1", "start": 458905, "stop": 459642, "strand": "-", "frame": 2, "gene": "nagB", "product": "glucosamine-6-phosphate deaminase", "db_xrefs": [ "EC:3.5.99.6", "GO:0004342", "GO:0006044", "RefSeq:WP_013354622.1", "SO:0001217", "UniParc:UPI0001A8C230", "UniRef:UniRef100_C4XEV1", "UniRef:UniRef50_C4XEV1", "UniRef:UniRef90_C4XEV1" ], "nt": "ATGAAAGTATATATTGAAAAAGATGCTTTAGCAGTAGCACAAAAAGCATTAAGTTTAATTAAGAATCAAATTACTAAAAAAGCAGATTCAAGAATTTGCTTTGCCACAGGTTCAACACCTTTAGATTTATATAAATTATTAATTGAAGATTATCAAAAAGGTAAAATTGATTTTAGTAAAATGGTTACTTTTAACTTGGATGAATACCTTGGATTAGATTTAAAAAATGAACATTCTTATCATTATTTTATGGATCATAATTTGTTTAATCACATTAATGTTAAAAGAGAAAATATCAATTTTCCTGATGTCATAAAATATGAAAAAGAACATCAAAATGGTAGCTATGAACAAGTAATTGAAAAACTTGGAGGTATTGATTTTACTATTTTAGGTATTGGCGAAAACGGTCATATTGCATTTAATGAACCAGATAGTCAAGAACAAGATATTACAAGAGTAGTTGATTTAACTGAAAGCACAATTAATGCAAACAAAAGATTTTTTGCTAGTGAAAAAGATGTTCCTCGTAAAGCAGTAACTATGGGAATAGGAACTATTTTAAAAAGTAAAAAAATTATGTTATTAGCAACCGGGCCTAAAAAAGCTCAAGCAATTTATGATACTTTACTTAAACCAGTAAATTCAAATTGTCCAGCCAGCTTTTTACAAAATCATAAAGATGTAACAATTATTGTTGATGAATTATCAGCGAAATTAATCAAAAATAAAATTTAA", "aa": "MKVYIEKDALAVAQKALSLIKNQITKKADSRICFATGSTPLDLYKLLIEDYQKGKIDFSKMVTFNLDEYLGLDLKNEHSYHYFMDHNLFNHINVKRENINFPDVIKYEKEHQNGSYEQVIEKLGGIDFTILGIGENGHIAFNEPDSQEQDITRVVDLTESTINANKRFFASEKDVPRKAVTMGIGTILKSKKIMLLATGPKKAQAIYDTLLKPVNSNCPASFLQNHKDVTIIVDELSAKLIKNKI", "aa_hexdigest": "a3a0e82afc87760eaa544d701bc3c27e", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C230", "ncbi_nrp_id": "WP_013354622.1", "uniref100_id": "UniRef100_C4XEV1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C230", "RefSeq:WP_013354622.1", "UniRef:UniRef100_C4XEV1" ] }, "ips": { "uniref100_id": "UniRef100_C4XEV1", "uniref90_id": "UniRef90_C4XEV1" }, "psc": { "uniref90_id": "UniRef90_C4XEV1", "gene": "nagB", "product": "glucosamine-6-phosphate deaminase", "ec_ids": [ "3.5.99.6" ], "uniref50_id": "UniRef50_C4XEV1", "go_ids": [ "GO:0004342", "GO:0006044" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEV1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEV1" ], "product": "Glucosamine-6-phosphate deaminase" }, "genes": [ "nagB" ], "id": "OAIKOHHJFB_837", "locus": "GCA000209735_00818" }, { "type": "cds", "sequence": "contig_1", "start": 459655, "stop": 460140, "strand": "-", "frame": 2, "gene": "nagA", "product": "N-acetylglucosamine-6-phosphate deacetylase", "db_xrefs": [ "EC:3.5.1.25", "GO:0006044", "GO:0008448", "KEGG:K01443", "SO:0001217", "UniRef:UniRef50_L0RWM1", "UniRef:UniRef90_C4XEV2" ], "nt": "ATGAGTGGAATTGATTCAAGAAAACCGGGAATCTTAGAAGCTTCATTAGAAAGACATGATGTTTATAGTGAATTAATTGTTGATTTTCATCACATTTGTCAAGAAAGTATAGTTTTTACGCTTAATCATAAAGATATAAACCACTTAATGTGTGTTAGCGATGCAATTCGACCTGCTTATGGACCTGATGGTAAAAGCATTTCAGGTGGAATTAATATTGAAAAACATGGTTTAACAATTTATTTAGAGGGCACAACAACTATAGCTGGAAGTGGAATTTGCTTACATGATTCATTTAAAAATTTAGCTTCATTAGATTTAGATTATCGTGATATTGTTAAATTAACTTCATATAATGCAGCTAAAGCTCATAATTTAAAAGATCGAGCACAAATTAAAGCAAATAAATTAGCAGATTTAGTTTTAATGGATTTAAAAACTTTAGATATTAAAGAAGTTTATATTGATGGGCAAAAAGTCAAATAA", "aa": "MSGIDSRKPGILEASLERHDVYSELIVDFHHICQESIVFTLNHKDINHLMCVSDAIRPAYGPDGKSISGGINIEKHGLTIYLEGTTTIAGSGICLHDSFKNLASLDLDYRDIVKLTSYNAAKAHNLKDRAQIKANKLADLVLMDLKTLDIKEVYIDGQKVK", "aa_hexdigest": "c2794937e22f911759187dc2058a9756", "start_type": "ATG", "rbs_motif": null, "genes": [ "nagA" ], "truncated": "3-prime", "pseudogene": { "start": 459655, "stop": 460440, "observations": { "mutation": [ 460149, 460365 ], "stop": [ 460149, 460365 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEV2", "query_cov": 1.0, "subject_cov": 0.4410958904109589, "identity": 1.0, "score": 323.0, "evalue": 8.47e-109, "gene_start": 1, "gene_end": 161, "reference_start": 205, "reference_end": 365, "reference_sequence": "MLIKNVKIVNHNQIIECADIEFSKKITKITPTKNKAQYLLVPGFIDTHIHGFMNKDVMDSSAAVEDISLNLARNGITSFMPTAMTASWENIQIALKNIALAKCQGAKNIGIHIEGPFIGEAKKGAHKLEWLRRATREDILEMLNSSNHQLKKISYDPLMVEEKLVPFMVQNDIMPSIGHSAASYDQAMKYYDLGSTNTCHLWNAMSGIDSRKPGILEASLERHDVYSELIVDFHHICQESIVFTLNHKDINHLMCVSDAIRPAYGPDGKSISGGINIEKHGLTIYLEGTTTIAGSGICLHDSFKNLASLDLDYRDIVKLTSYNAAKAHNLKDRAQIKANKLADLVLMDLKTLDIKEVYIDGQKVK" }, "paralog": false, "identity": 0.9923371647509579, "score": 517.0, "evalue": 1.27e-187, "description": "Internal stop codon at 460149, 460365. Nonsense mutation around 460149, 460365.", "psc": { "uniref90_id": "UniRef90_C4XEV2", "gene": "nagA", "product": "N-acetylglucosamine-6-phosphate deacetylase", "ec_ids": [ "3.5.1.25" ], "uniref50_id": "UniRef50_L0RWM1", "kegg_orthology_id": "K01443", "go_ids": [ "GO:0006044", "GO:0008448" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEV2", "EC:3.5.1.25", "UniRef:UniRef50_L0RWM1", "KEGG:K01443", "GO:0006044", "GO:0008448" ] }, "pscc": { "uniref50_id": "UniRef50_L0RWM1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RWM1" ], "product": "N-acetylglucosamine-6-phosphate deacetylase" } }, "id": "OAIKOHHJFB_838", "locus": "GCA000209735_00819" }, { "type": "cds", "sequence": "contig_1", "start": 460147, "stop": 460329, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAATAGTTCAAATCATCAATTGAAAAAAATTAGTTATGATCCTTTAATGGTTGAAGAAAAATTAGTTCCTTTTATGGTTCAAAATGACATTATGCCTTCAATTGGTCACTCAGCTGCAAGCTATGATCAAGCTATGAAATACTATGATTTGGGTTCAACTAATACTTGTCACTTATGA", "aa": "MLNSSNHQLKKISYDPLMVEEKLVPFMVQNDIMPSIGHSAASYDQAMKYYDLGSTNTCHL", "aa_hexdigest": "0a06cc54445ef5fe5a341ea80c828741", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6806.729700000002, "isoelectric_point": 5.756505393981934 }, "id": "OAIKOHHJFB_839", "locus": "GCA000209735_00820" }, { "type": "cds", "sequence": "contig_1", "start": 460489, "stop": 460752, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01979.26" ], "nt": "ATGCTAATAAAAAACGTTAAAATAGTTAATCACAACCAAATTATTGAGTGTGCTGACATTGAGTTTAGTAAAAAAATTACTAAAATAACTCCAACTAAAAATAAGGCTCAATATTTATTAGTTCCAGGCTTTATTGACACTCATATTCATGGTTTTATGAATAAGGATGTAATGGATAGTAGTGCAGCAGTTGAAGATATTAGTTTAAATTTAGCTAGAAATGGAATTACTAGTTTTATGCCTACAGCAATGACAGCAAGTTGA", "aa": "MLIKNVKIVNHNQIIECADIEFSKKITKITPTKNKAQYLLVPGFIDTHIHGFMNKDVMDSSAAVEDISLNLARNGITSFMPTAMTAS", "aa_hexdigest": "6961c50969b1e300b5bdb9937c776592", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01979.26", "name": "Amidohydro_1", "length": 58, "aa_cov": 0.5287356321839081, "hmm_cov": 1.0, "evalue": 1.366428838013047e-07, "score": 27.16958236694336, "start": 40, "stop": 85 } ], "seq_stats": { "molecular_weight": 9636.156699999994, "isoelectric_point": 7.90738582611084 }, "id": "OAIKOHHJFB_840", "locus": "GCA000209735_00821" }, { "type": "cds", "sequence": "contig_1", "start": 460985, "stop": 461398, "strand": "+", "frame": 2, "gene": null, "product": "Phosphoglycerate kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A378MFN7" ], "nt": "ATGAAAAAAACTATTGACGATTTAGTATTAAAAGGCAAGAAAGTTTTAATGAGAGTTGACTTTAATGTGCCTCTTAAAGATGGTGTTATTACATCAAATAAGAGAATTGTAGCTGCTTTACCTACAATTGAAAAAGTTATCAAGGAAAAAGGAAAATTAATTTTATTTTCTCACTTAGGACGCGTTAAAAGTGAAGAAGATAAAGCTAAAAACAACTTATTACCTGTAGCTAATGAATTAGCCAGATTACTTAAAAAACCAGTACTTTTTGTTGACTCAACTAGAGGCGCTGAATTAGAAAAAGCAATTGCTAAAATGGAACCAGGCGAAGTGATTTTAGTTCAAAACACTCGTTATGAAGACTTAAACGAAAAAGCTGAAAGTAAAAACAATCCTATTTTAGGAAAATACTGA", "aa": "MKKTIDDLVLKGKKVLMRVDFNVPLKDGVITSNKRIVAALPTIEKVIKEKGKLILFSHLGRVKSEEDKAKNNLLPVANELARLLKKPVLFVDSTRGAELEKAIAKMEPGEVILVQNTRYEDLNEKAESKNNPILGKY", "aa_hexdigest": "34e8d9ace610e7b2b32a6d05df889a28", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A378MFN7", "query_cov": 0.9343065693430657, "subject_cov": 0.8767123287671232, "identity": 0.617, "score": 151.0, "evalue": 1.64e-44, "valid": false, "product": "Phosphoglycerate kinase", "ec_ids": [ "2.7.2.3" ], "uniref50_id": "UniRef50_A0A378MFN7", "kegg_orthology_id": "K00927" }, "pscc": { "uniref50_id": "UniRef50_A0A378MFN7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A378MFN7" ], "product": "Phosphoglycerate kinase" }, "genes": [], "id": "OAIKOHHJFB_841", "locus": "GCA000209735_00822" }, { "type": "cds", "sequence": "contig_1", "start": 461510, "stop": 461929, "strand": "+", "frame": 2, "gene": null, "product": "phosphoglycerate kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002ACD8294" ], "nt": "ATGGAAAAAGAAGTTAATGCTTTAACTAAAGCTATCAAAAACCCTAAGAAACCTTATGTAGCTATTATTGGTGGAGCTAAAGTTAGTGATAAAATTCAAGTTTTAGAAAACTTAATTCCACTTGTAGACAAAATGATTATTGGTGGTGGAATGGCATATACATTCTTAAAAAGCCAAGGATACAAAATTGGTAAAAGCTTAGTAGAAGATGAATTTATTCCTTTTGCTACAGAATTCTTAAAGAAAAACAAAGATAAAATCATCTTACCGGTTGATCATGCATGTGCAAAAGAATTTGCCGATGTAGAACCAGTTGTTTATGGATTAGACATTGATGACAATTACGATGGACTTGACTTAGGACCTAAATCAATCGAAAAATTCGTATCTGCTTTAGAAGGCGCAAAAACAGTTGTTTGA", "aa": "MEKEVNALTKAIKNPKKPYVAIIGGAKVSDKIQVLENLIPLVDKMIIGGGMAYTFLKSQGYKIGKSLVEDEFIPFATEFLKKNKDKIILPVDHACAKEFADVEPVVYGLDIDDNYDGLDLGPKSIEKFVSALEGAKTVV", "aa_hexdigest": "c0d9705ec49c9e350b532b7ff52e19a5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002ACD8294", "query_cov": 0.8992805755395683, "subject_cov": 0.984251968503937, "identity": 0.536, "score": 124.0, "evalue": 4.22e-34, "valid": false, "product": "phosphoglycerate kinase", "ec_ids": [ "2.7.2.3" ], "uniref50_id": "UniRef50_UPI002ACD8294", "kegg_orthology_id": "K00927" }, "pscc": { "uniref50_id": "UniRef50_UPI002ACD8294", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002ACD8294" ], "product": "phosphoglycerate kinase" }, "genes": [], "id": "OAIKOHHJFB_842", "locus": "GCA000209735_00823" }, { "type": "cds", "sequence": "contig_1", "start": 461939, "stop": 462169, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00162.24" ], "nt": "ATGGGTGTTACAGAATTTGAAAACTACAAAACAGGTACTTTAGAAGTTGCTAAAGCTGTTGCTGCATTAAAAGGTTGCTACTCAATTGTTGGTGGTGGTGACTCAGTTGCTGCTGTTCAAAAACTTAAAATGGAAAGCAAATTCTCACATGTTTCAACAGGCGGTGGAGCAAGTCTTGAATTCTTACAAGGTATTGCACTTCCAGGTGTAGAAGCTATTCAAAATAAATAG", "aa": "MGVTEFENYKTGTLEVAKAVAALKGCYSIVGGGDSVAAVQKLKMESKFSHVSTGGGASLEFLQGIALPGVEAIQNK", "aa_hexdigest": "df63e89285e91b8aa4874a865f3a43e0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002A7EA9FD", "query_cov": 1.0263157894736843, "subject_cov": 0.9176470588235294, "identity": 0.5770000000000001, "score": 85.5, "evalue": 2.57e-20, "valid": false, "product": "phosphoglycerate kinase", "ec_ids": [ "2.7.2.3" ], "uniref50_id": "UniRef50_A0A166IH01", "kegg_orthology_id": "K00927" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00162.24", "name": "PGK", "length": 65, "aa_cov": 0.8421052631578947, "hmm_cov": 1.0, "evalue": 5.291765248958528e-25, "score": 84.2491226196289, "start": 1, "stop": 64 } ], "seq_stats": { "molecular_weight": 7790.853999999998, "isoelectric_point": 6.544691658020019 }, "id": "OAIKOHHJFB_843", "locus": "GCA000209735_00824" }, { "type": "cds", "sequence": "contig_1", "start": 462224, "stop": 462349, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAATTGCAAGATACAAAATTCATTACAGGAATTATGATTAGCACTAATAAAATTTCATTAGTTATTGGTGATTATGCACGTAAGTTAGCTCTTGAAATGAATGAATATTTAGAAAAGTAA", "aa": "MDKLQDTKFITGIMISTNKISLVIGDYARKLALEMNEYLEK", "aa_hexdigest": "9cc1c20f22f06a4ddd509858ca720f13", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4735.540999999999, "isoelectric_point": 6.060423469543457 }, "id": "OAIKOHHJFB_844", "locus": "GCA000209735_00825" }, { "type": "cds", "sequence": "contig_1", "start": 462750, "stop": 463034, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCAAAAAAAGATTATTAAGTGGTATTAAACCAACAGGTGATTTAACCCTAGGAAATTACATTGGAGCTTTACGTAATTTTGTTAAATTACAAGAAAATTATGATGAAGCTTACTATTTTGTAGCTGATTTACATGCTTTAACAACAGGTTATGTTGATCCTAAAGAGTTATACCAAGCAAGAAGAGAAATAGTAACAATGTATTTAGCATGTGGCTTAGATCCTAAAAAAAGCACAATATTTTACCAAAGTGATGTTGTTGAACATACTTTGGCTCAATGA", "aa": "MSKKRLLSGIKPTGDLTLGNYIGALRNFVKLQENYDEAYYFVADLHALTTGYVDPKELYQARREIVTMYLACGLDPKKSTIFYQSDVVEHTLAQ", "aa_hexdigest": "12e4e8eaa0cebc5bd1158ac7bc69e37f", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10710.146499999997, "isoelectric_point": 6.712594032287597 }, "id": "OAIKOHHJFB_845", "locus": "GCA000209735_00826" }, { "type": "cds", "sequence": "contig_1", "start": 463038, "stop": 463751, "strand": "+", "frame": 3, "gene": null, "product": "Tryptophan--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PAD4" ], "nt": "ATGACTACTGAAATATCAATTGGTGAATTAAGCAGAATGACTCAATTTAAAGACAAAAGTCAAAAGATGTCAAAACAAGCTAATGGTACAGAAAAAATTCCAGTTGGCTTATTAATGTATCCAGTTTTAATGGCAGCAGATATTTTAATTTATAATCCTGACTTTGTTCCTATTGGTGAAGACCAAAAGCAACACTTAGAGTTAGCACGGACTATAGCAGAAAGATTGAACCGTGATTATAAAACGAACTTTAAGTTAGCAGAAGGAATTATTCCTCCAGTAGGCGCTAGAATTATGTCACTAACTGATCCAACTAAAAAAATGTCAAAAAGTGACAAAGGCACTAAAGCAACAATTTATCTTCATGATGATCCTGAAGTTGCTTACAAAAAAATTATGAAAAGCGTCACAGACAGTGAAAACAAAGTTTACATTTCGGAAGATAAACCTGGAGTATTAAACTTATTAAATATTTATGCTTCACTAACTAATAAAACTTTAAAAGAAGCAGAAAAAGAATTTAAAGACAAAGACTATGCTCAATTCAAGAGTGCTGTAGCAATAGTTGTCAAAGATGAATTAACTAAAATTCAAGCTAATTATGCAAAAGCAACCAAAATGGTTGATAAAGTTGTGGCCGAAGGCGCACAAAAAGCTCAAGCAATCTGTCAACCTATTGTTGAAGATTTGATGAAGAAAATGGGGTATAGATAA", "aa": "MTTEISIGELSRMTQFKDKSQKMSKQANGTEKIPVGLLMYPVLMAADILIYNPDFVPIGEDQKQHLELARTIAERLNRDYKTNFKLAEGIIPPVGARIMSLTDPTKKMSKSDKGTKATIYLHDDPEVAYKKIMKSVTDSENKVYISEDKPGVLNLLNIYASLTNKTLKEAEKEFKDKDYAQFKSAVAIVVKDELTKIQANYAKATKMVDKVVAEGAQKAQAICQPIVEDLMKKMGYR", "aa_hexdigest": "6bb5c218c02ae2bd8eb195662435a1b9", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PAD4", "query_cov": 0.9915611814345991, "subject_cov": 0.9791666666666666, "identity": 0.574, "score": 260.0, "evalue": 1.1e-84, "valid": false, "gene": "trpS", "product": "tryptophan--tRNA ligase", "ec_ids": [ "6.1.1.2" ], "uniref50_id": "UniRef50_A0A3B0PAD4", "go_ids": [ "GO:0000166", "GO:0004830", "GO:0005524", "GO:0006436" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PAD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PAD4" ], "product": "Tryptophan--tRNA ligase" }, "genes": [], "id": "OAIKOHHJFB_846", "locus": "GCA000209735_00827" }, { "type": "cds", "sequence": "contig_1", "start": 464015, "stop": 464449, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07973.19" ], "nt": "ATGAAAATACAAGTAGACAAGTTACTTAATCATACAACAAGTCACCTTTTAGGTGCCGCAGTTGAAAAACTTTATCCAGAAGTTAAATTAGGTTTTGGACCTGCAACCGAAGAAGGTTTTTACTATGATTTCGAATTTAAAGAACCTTTAAGTGTTAATGAATTAAGTAAGATTGAAAAGTTAATGAAAAAATTAGCAAGCAGGAATCTGGTAACTATTCAAATTCCTGAAAAAGATTATGACTTTAAAAACAAACCATATAAAAAAGAACTATATGATGAATTAAAAGCACAAGGCAAAAAGATTACTTTTTATGCTCTTCAAGATCCTCTTAATAAAGAAATAATATTTAAAGATTTATGTGCTGGTGGTCATGTTGAAAGTACAAAAGTTATTAAAAACTTTAAACTTTTAAGTATTGCTGGAGCTTACTGA", "aa": "MKIQVDKLLNHTTSHLLGAAVEKLYPEVKLGFGPATEEGFYYDFEFKEPLSVNELSKIEKLMKKLASRNLVTIQIPEKDYDFKNKPYKKELYDELKAQGKKITFYALQDPLNKEIIFKDLCAGGHVESTKVIKNFKLLSIAGAY", "aa_hexdigest": "4a2b8493d6aab52ff09f2d16689b2883", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07973.19", "name": "tRNA_SAD", "length": 37, "aa_cov": 0.2569444444444444, "hmm_cov": 0.8378378378378378, "evalue": 2.99362515832469e-08, "score": 29.6651611328125, "start": 103, "stop": 139 } ], "seq_stats": { "molecular_weight": 16492.008699999984, "isoelectric_point": 8.765911293029784 }, "id": "OAIKOHHJFB_847", "locus": "GCA000209735_00828" }, { "type": "cds", "sequence": "contig_1", "start": 464645, "stop": 465151, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "ATGTACATTCATAATGAAATTAAAAACTTAGTATTAAAAATGGACCGTAAATATGGATTTACTGAAGTTTTAACTCCACACTTTGGTTCAGAAGACTTATACAAAACCAGTGGTCATTTAGCTCACTATAAAGATGATATGTTTGCACCATTAGTTATTGAAAACGAAAGATTGATTCCTCGTCCAATGACTTGTCCTCACCACATTGTGTGTTATAACTTAGAAAAAAGATCATATCGTGATTTACCAATTAGATATTCAGAACAAAGTCAACTTTATCGTTATGAAAAATCAGGTGCTTTAACAGGACTTGAACGTGTTAGAGGAATGCTTTTAACTGAAGGTCACTTATTTGTTAGAGAAGATCAAATTGAACAAGAAATTAAAAGTATGTATCAATTAATTGAAGAAACATTAAAAATTTTCAATATTGATATTAGCTATATTTCACTTTCACTTCGTGATAAAAATGACAAAGAAAAATACTTTAATGACAATAAAATGTGA", "aa": "MYIHNEIKNLVLKMDRKYGFTEVLTPHFGSEDLYKTSGHLAHYKDDMFAPLVIENERLIPRPMTCPHHIVCYNLEKRSYRDLPIRYSEQSQLYRYEKSGALTGLERVRGMLLTEGHLFVREDQIEQEIKSMYQLIEETLKIFNIDISYISLSLRDKNDKEKYFNDNKM", "aa_hexdigest": "1733c70d9481a3e87c054679e4699825", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 105, "aa_cov": 0.6130952380952381, "hmm_cov": 0.9809523809523809, "evalue": 1.9315332599718043e-18, "score": 63.21187973022461, "start": 54, "stop": 156 } ], "seq_stats": { "molecular_weight": 20087.83030000001, "isoelectric_point": 6.385428810119628 }, "id": "OAIKOHHJFB_848", "locus": "GCA000209735_00829" }, { "type": "cds", "sequence": "contig_1", "start": 465167, "stop": 465457, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "ATGCTTAAAAAAGCTCTTGATGATATGGGAGTTAAGTATACAACCGTTGTTGGTGAAGCTGCTTTTTATGGACCAAAAATTGATATTCAAATTGACACAGCACTAGGCCATGAAATTACTGTTTCAACCATACAATTAGACTTTTTACAACCTAAAAACTTTGATATTACTTATACAGATAAAAATGGTAAAGAAGCAAGACCGGTTATGATTCACCGTGGTTTAATCGGAACTTATGAAAGATTTGTTGCTATTTTACTTGAACAAACTAAAGGTAACTTACCATTCTGA", "aa": "MLKKALDDMGVKYTTVVGEAAFYGPKIDIQIDTALGHEITVSTIQLDFLQPKNFDITYTDKNGKEARPVMIHRGLIGTYERFVAILLEQTKGNLPF", "aa_hexdigest": "1c0de043d776b6270796897da626c34f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0029CA135A", "query_cov": 0.8645833333333334, "subject_cov": 0.8217821782178217, "identity": 0.639, "score": 109.0, "evalue": 2.29e-29, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "ec_ids": [ "6.1.1.3" ], "uniref50_id": "UniRef50_Q71HR4", "kegg_orthology_id": "K01868" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 89, "aa_cov": 0.8645833333333334, "hmm_cov": 0.9775280898876404, "evalue": 1.5101029269001276e-15, "score": 53.78683853149414, "start": 7, "stop": 89 } ], "seq_stats": { "molecular_weight": 10774.359399999992, "isoelectric_point": 6.056899452209472 }, "id": "OAIKOHHJFB_849", "locus": "GCA000209735_00830" }, { "type": "cds", "sequence": "contig_1", "start": 465611, "stop": 465763, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25" ], "nt": "ATGTCAAAATCAAAATACCAAGTGATTTTGGGGGCTAATGAAGAAAAAGACAAAACAATTTCTTATCGTGAATATGGTAAAGAAGATACAAAAACTGTGACAGTTGAAGAATTTATCAACATTCTTACAAAATTAAGAAATAACTATGAATAG", "aa": "MSKSKYQVILGANEEKDKTISYREYGKEDTKTVTVEEFINILTKLRNNYE", "aa_hexdigest": "1018cd1ce96b3544e9adc90a81fea955", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 42, "aa_cov": 0.84, "hmm_cov": 1.0, "evalue": 2.2744348943774433e-06, "score": 23.8287296295166, "start": 3, "stop": 44 } ], "seq_stats": { "molecular_weight": 5918.595899999999, "isoelectric_point": 6.2491857528686525 }, "id": "OAIKOHHJFB_850", "locus": "GCA000209735_00831" }, { "type": "cds", "sequence": "contig_1", "start": 466319, "stop": 466846, "strand": "+", "frame": 2, "gene": null, "product": "Isochorismatase-like domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354629.1", "SO:0001217", "UniParc:UPI0001E330EE", "UniRef:UniRef100_C4XEV9", "UniRef:UniRef50_A0A077L748", "UniRef:UniRef90_C4XEV9" ], "nt": "ATGAAAAAAATAATCTGTGTTATTGACATGCTTGAAGGTTTTTGCAATGAAGGCGCTTTGGCAAGTCCAATAGTTAAAAAAATAATACCTAATATTGAAAAAGTATTAAAAGACAATAAAAAAGAAGACAATTTATTTATTTGCGATAGTCATAATACTTTTGATTTGGAGATGAAACAATATCCACTTCATTGCCTTAAAGACACAAAAGAGGCTGAAATTGTTAAGGAATTAAAACCTTATGTTAAAAGTGTTTTAGAAAAAAATTCAACTAATGCATTTCATCTTTTTGACAAAAAACTAATTGAAAAATATGATCAATTTGTTTTGACTGGTTGTTGCACAGATATTTGTGTTTTACAATTTGCTTTGAGTTTAAAAACATATTTAAATGAAAATAGAATTGATAAAAAAGTTATTGTTTTAAAAGATGCTGTTGCCACATTTGATGCTCCAGGACATAATGCTCAACAATTTCACAATTTCACCTTAAACTTAATGGCTAATGCAGGCATTGAAATTCAATAA", "aa": "MKKIICVIDMLEGFCNEGALASPIVKKIIPNIEKVLKDNKKEDNLFICDSHNTFDLEMKQYPLHCLKDTKEAEIVKELKPYVKSVLEKNSTNAFHLFDKKLIEKYDQFVLTGCCTDICVLQFALSLKTYLNENRIDKKVIVLKDAVATFDAPGHNAQQFHNFTLNLMANAGIEIQ", "aa_hexdigest": "d5053b3198c14a52349f1c515f70fa1e", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E330EE", "ncbi_nrp_id": "WP_013354629.1", "uniref100_id": "UniRef100_C4XEV9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330EE", "RefSeq:WP_013354629.1", "UniRef:UniRef100_C4XEV9" ] }, "ips": { "uniref100_id": "UniRef100_C4XEV9", "uniref90_id": "UniRef90_C4XEV9" }, "psc": { "uniref90_id": "UniRef90_C4XEV9", "product": "Isochorismatase-like domain-containing protein", "uniref50_id": "UniRef50_A0A077L748" }, "pscc": { "uniref50_id": "UniRef50_A0A077L748", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L748" ], "product": "Isochorismatase-like domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_851", "locus": "GCA000209735_00832" }, { "type": "cds", "sequence": "contig_1", "start": 466849, "stop": 467130, "strand": "+", "frame": 1, "gene": null, "product": "Smr domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354630.1", "SO:0001217", "UniParc:UPI0001E330EF", "UniRef:UniRef100_C4XEW0", "UniRef:UniRef50_C4XEW0", "UniRef:UniRef90_C4XEW0" ], "nt": "ATGACTAAAACAATTGATTTACATAAATTAAATGTTGAACAAGCTACTTCAAAAGTTATTTTAGCTTTAAGTGAAGCCCAAGAGAAAAAACTTACTTCTTTAGAAATAATAACTGGTTATGGTAGTGGTGCTCTCAGAGCAAGAACTCTAGAATTACTAGAATCTGAAAATTTAACTTATATTGAAGAAGGACCAATGGTAATTGTTTATTTTTTTAATAATAATGAAGATGAAAACGATGATAATTTTTCAGATATAGAATATCAAAAGAAGTTCCAATAA", "aa": "MTKTIDLHKLNVEQATSKVILALSEAQEKKLTSLEIITGYGSGALRARTLELLESENLTYIEEGPMVIVYFFNNNEDENDDNFSDIEYQKKFQ", "aa_hexdigest": "e7b8c658c22a427049059c3588eff924", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E330EF", "ncbi_nrp_id": "WP_013354630.1", "uniref100_id": "UniRef100_C4XEW0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330EF", "RefSeq:WP_013354630.1", "UniRef:UniRef100_C4XEW0" ] }, "ips": { "uniref100_id": "UniRef100_C4XEW0", "uniref90_id": "UniRef90_C4XEW0" }, "psc": { "uniref90_id": "UniRef90_C4XEW0", "product": "Smr domain-containing protein", "uniref50_id": "UniRef50_C4XEW0" }, "pscc": { "uniref50_id": "UniRef50_C4XEW0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEW0" ], "product": "Smr domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_852", "locus": "GCA000209735_00833" }, { "type": "cds", "sequence": "contig_1", "start": 467185, "stop": 467370, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGACTTTTATTTAATATACTTAGCTTTACAATTATTAGTTCCTTTTGGACTTGAAATATCAGTCAAAGAAGAAATTGTCAACAAGCCTTTAGAAAGTGATTTAACTTTTGAAGGTGAAAAAGAACAAAATAAAGATAATAATCAGGAAAATAATCAAACACAATCAAAAAATAGTAAATAG", "aa": "MDDFYLIYLALQLLVPFGLEISVKEEIVNKPLESDLTFEGEKEQNKDNNQENNQTQSKNSK", "aa_hexdigest": "6cba0285e07590f901d81f9ad8ff4fde", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7090.793200000002, "isoelectric_point": 4.339623069763184 }, "id": "OAIKOHHJFB_853", "locus": "GCA000209735_00834" }, { "type": "cds", "sequence": "contig_1", "start": 467411, "stop": 467605, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTGGCTTCAAAGATTAAATCAATTAATTCAAAAGGAATGTCAAATCAAATAAATGCTTCTGTATTTCTAATTAAGAATGATAAATTATTTAATAAATATTTTTTATTCGCTTTAGTGTTAGGAACACTAATTTGGCTAATAGTCAATTCATTGAAAATTTCATTTAAGAATTCTAAATTATTTTTTTGA", "aa": "MVLASKIKSINSKGMSNQINASVFLIKNDKLFNKYFLFALVLGTLIWLIVNSLKISFKNSKLFF", "aa_hexdigest": "2cb561f63287978b91bc3b3c5172e282", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7300.754299999998, "isoelectric_point": 10.368079566955565 }, "id": "OAIKOHHJFB_854", "locus": "GCA000209735_00835" }, { "type": "cds", "sequence": "contig_1", "start": 467878, "stop": 468228, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTATTTGGTATTTCATTAACTTTAACTATCCCTGGCTTAATTGTTCTTTTAATAGGACAAAAAGCTAAAAAATATGTGCTGAAAGATGTTATTACCAAATTTGATAAATATAAATTAATCAAAAAATTATTTAACTTCTTTACTCTTGGTTACAAAGAAAATATCGTTGATGAAAATAACAAAATTCACAAAAATTATGACTTAATCAATTTTTATAAAAACACACATAATTTCAAAAATTTTGAATTTGGTTTTGCTCCAATCCATAAAAAATATGAACAATATGAAGCTTTAACTGATAACCAATATTTAAAGATGCAAAATGTTTACTACGAAGGCAACATTTGA", "aa": "MLFGISLTLTIPGLIVLLIGQKAKKYVLKDVITKFDKYKLIKKLFNFFTLGYKENIVDENNKIHKNYDLINFYKNTHNFKNFEFGFAPIHKKYEQYEALTDNQYLKMQNVYYEGNI", "aa_hexdigest": "7fff1fc8bc39a78472e457ec01c4bc53", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13861.003299999984, "isoelectric_point": 9.357150840759278 }, "id": "OAIKOHHJFB_855", "locus": "GCA000209735_00836" }, { "type": "cds", "sequence": "contig_1", "start": 468367, "stop": 468498, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGTATCAAATAAGTTAATTTCAAAAGAAGCTTTTATTCGTTCTAAACGTCCAACAATAAACAAATTAACTACTCAATTAATTAATAATTATTATGCTGATAAAAAGAAAAGCAAAAGGATTAATTGA", "aa": "MSVSNKLISKEAFIRSKRPTINKLTTQLINNYYADKKKSKRIN", "aa_hexdigest": "69bbbeab83aa6042f17205e2270e75ba", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5041.871600000003, "isoelectric_point": 10.573346900939939 }, "id": "OAIKOHHJFB_856", "locus": "GCA000209735_00837" }, { "type": "cds", "sequence": "contig_1", "start": 468700, "stop": 469086, "strand": "-", "frame": 2, "gene": null, "product": "Deoxyribose-phosphate aldolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A139MP28" ], "nt": "ATGCTTACAAGTGCTAAGGCATTTGAAGCTAAAGAAGCTATTAAAGCTGGTGCTGATGAAATTGACATGGTTATTCAAATTGGAAAAATGAAAATGGGTGACTATGACTATGTTTTAGAAGACATTAAAGCGGTTAAAAAAGCATGCGGCAAACATGTTTTAAAAGTTATTATTGAAACAGCTTTATTAACAGAAGCTGAAATTAGAAAAGCTACTGAAATCGTTATGGCTTCTGATGCTGAATTCATTAAAACTTCAACAGGTTTCTCAACAAGAGGCGCAACACTTGAAGATGTTAAAATCATGAAAAGTGTTTGTGGAGACAAATTATTAATCAAAGCTGCTGGTGGAATCAGCAACAAAGATGACATGATCAACATGGCTTAA", "aa": "MLTSAKAFEAKEAIKAGADEIDMVIQIGKMKMGDYDYVLEDIKAVKKACGKHVLKVIIETALLTEAEIRKATEIVMASDAEFIKTSTGFSTRGATLEDVKIMKSVCGDKLLIKAAGGISNKDDMINMA", "aa_hexdigest": "931b0c9145b6fe36632b5b56fb5c8497", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003672295A", "query_cov": 0.984375, "subject_cov": 0.8181818181818182, "identity": 0.603, "score": 149.0, "evalue": 8.51e-44, "valid": false, "gene": "deoC", "product": "deoxyribose-phosphate aldolase", "ec_ids": [ "4.1.2.4" ], "uniref50_id": "UniRef50_A0A139MP28", "kegg_orthology_id": "K01619", "go_ids": [ "GO:0004139", "GO:0005737", "GO:0009264" ] }, "pscc": { "uniref50_id": "UniRef50_A0A139MP28", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A139MP28" ], "product": "Deoxyribose-phosphate aldolase" }, "genes": [], "id": "OAIKOHHJFB_857", "locus": "GCA000209735_00838" }, { "type": "cds", "sequence": "contig_1", "start": 469162, "stop": 469293, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAATTAATTACAACAAAATGATTGATCACACTTATTTAAAAGCTGATGCGACAAATAAAGAAATTGATAATTTATTAGATGAATCCAAGAAATATGACTTCAAAACTGTTTGTGTTAATTCATCATGA", "aa": "MQINYNKMIDHTYLKADATNKEIDNLLDESKKYDFKTVCVNSS", "aa_hexdigest": "c1efecf7749e121598ca40dd3d531873", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5042.630900000001, "isoelectric_point": 5.553703880310058 }, "id": "OAIKOHHJFB_858", "locus": "GCA000209735_00839" }, { "type": "cds", "sequence": "contig_1", "start": 469316, "stop": 469540, "strand": "-", "frame": 1, "gene": null, "product": "Pyrimidine-nucleoside phosphorylase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDY4", "UniRef:UniRef90_A0A3P8MDY4" ], "nt": "ATGAAACTTGGCGCCGGCCGTGCTAAAAAAGAAGATAGTATTGACTTTGAAGCAGGAATTACTCTTCACGCTAAAACAAATCATAAAGTAACTAAAGGGCAAAAACTATTTACTCTTTATTCTTCAAATCCGATTGACAAGAATTTAGTTAAAGAATTAGAAACAGGTTACAAAATTGGCAATTCAAAAGTTGAAAATAAGATTATAATTGCTAAGTTGAAATAA", "aa": "MKLGAGRAKKEDSIDFEAGITLHAKTNHKVTKGQKLFTLYSSNPIDKNLVKELETGYKIGNSKVENKIIIAKLK", "aa_hexdigest": "96115f3718663ff416c9a1c04620841f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3P8MDY4", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.905, "score": 132.0, "evalue": 3.06e-39, "valid": true, "product": "Pyrimidine-nucleoside phosphorylase", "uniref50_id": "UniRef50_A0A3P8MDY4" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MDY4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDY4" ], "product": "Pyrimidine-nucleoside phosphorylase" }, "genes": [], "id": "OAIKOHHJFB_859", "locus": "GCA000209735_00840" }, { "type": "cds", "sequence": "contig_1", "start": 469838, "stop": 470611, "strand": "-", "frame": 1, "gene": null, "product": "thymidine phosphorylase", "db_xrefs": [ "EC:2.4.2.4", "GO:0006206", "SO:0001217", "UniRef:UniRef50_R5VCS9", "UniRef:UniRef90_A0A3P8LBE3" ], "nt": "ATGCGTGTCGTTGATTTAATCGAAAAGAAAAGAGAAGGTAAAGAATTAACAACTCAAGAAATTGAATTCTTAATTACTTCTTATGTATCAGGTAATACACCTGATTACCAAATGGCTGCTTTTATAATGGCAGTTATGTTCCAAGGAATGGCAAAAAAAGAATTAGCAACTTTTACTAAAACCATGATGCACTCAGGTGAAGTTATTGATTTAAGTGAAATTCCAGGGATTAAAGTTGATAAACACTCAACAGGTGGAGTTGGAGATAAAACTACTTTAGCAGTTGCTCCTATTTGTGCCGCTTTAGGTGCTCCAGTAGCGAAAATGAGTGGTAGAGGTTTAGGCCACACTGGTGGAACAATTGATAAACTTGAATCTATTCCAGGATTTCATGTTGAATTAACTGAACGTCAATTTATTGAACAAGTTAAAAAACATAATATTTCAGTTATTGTTCAATCAAAAGAATTAGTACCAGCTGATAAAAAACTTTATGCTTTAAGAGATGTTACTGCTTGTGTTAACTCAATTCCTCTTATTGCTTCAAGTATTATGTCTAAAAAATTAGCTACAGGTTCAAATGCTATTTTATTAGATGTTAAATGCGGTAAAGGCGCCTTCATGAAAAATGAAGACCAAGCTAAAGAATTAGCTAAAACTATGATTTCAATTGGTAAAGAATTAAACGTTGATGTTCGTGCAGAAATTACCAACATGAACCGTCCAATTGGTTGTGAAATTGGAAACAAAAATGAAGTTTTAGAAGCTATTTGA", "aa": "MRVVDLIEKKREGKELTTQEIEFLITSYVSGNTPDYQMAAFIMAVMFQGMAKKELATFTKTMMHSGEVIDLSEIPGIKVDKHSTGGVGDKTTLAVAPICAALGAPVAKMSGRGLGHTGGTIDKLESIPGFHVELTERQFIEQVKKHNISVIVQSKELVPADKKLYALRDVTACVNSIPLIASSIMSKKLATGSNAILLDVKCGKGAFMKNEDQAKELAKTMISIGKELNVDVRAEITNMNRPIGCEIGNKNEVLEAI", "aa_hexdigest": "81c73730e64c57a6a3bb4398ce8f3e9f", "start_type": "ATG", "rbs_motif": "GGAGG", "psc": { "uniref90_id": "UniRef90_A0A3P8LBE3", "query_cov": 1.0, "subject_cov": 0.9178571428571428, "identity": 0.914, "score": 454.0, "evalue": 4.1e-160, "valid": true, "product": "thymidine phosphorylase", "ec_ids": [ "2.4.2.4" ], "uniref50_id": "UniRef50_R5VCS9", "go_ids": [ "GO:0006206" ] }, "genes": [], "id": "OAIKOHHJFB_860", "locus": "GCA000209735_00841" }, { "type": "cds", "sequence": "contig_1", "start": 470625, "stop": 471329, "strand": "-", "frame": 3, "gene": "deoD", "product": "purine-nucleoside phosphorylase", "db_xrefs": [ "EC:2.4.2.1", "GO:0004731", "GO:0006139", "KEGG:K03784", "RefSeq:WP_013526885.1", "SO:0001217", "UniParc:UPI0001A8C23D", "UniRef:UniRef100_C4XEW4", "UniRef:UniRef50_S5MIP4", "UniRef:UniRef90_C4XEW4" ], "nt": "ATGACACCACACATTAATGCCAAAGAAGGTGATATTGCTAAGACAGTTTTAATGCCAGGAGATCCTTTAAGAGCTAAATTTATTGCTGAAACATTTTTAGACAAAGGATATAGACTTGTTAATACTGTCAGAAACATGTACATGTATACAGGTACATATAAAGGTCGTCCAGTAACAATCGCTGGAAGTGGAATGGGTTGTCCATCAATTGGGATCTATTCATATGAATTATTCAAATTCTACAATGTAGATAACATTATTAGAATCGGTACAACTGGTTCATACAAAAAAGAAATCAAACTATATGAAACGATTTTAGCAACTGAAGCTTATGCAGATGGTGCTAGTTATCGTCGTTTAGTTTTAGGAGACAAAAGCCATGTAGCTTTACCTAGTGCTAAATTAAATAAAGAAATTGAAAAAGTGGCTAAAAATTTGAACATTAAATTAACTTTAGGCCGTGTACACTCTTCAGATGTATTCTATTCATCAGTTCCTCTTCAACAAAGAATCGATGAAACACATGCGCTTTGTGTTGAAATGGAATCATATGCTTTATTTACTAATGCTGAAAAATTAGGTAAAAATGCAGCATGTCTTTTAACAGTTAGTGACAACTTAATTACAAATGACGAAACAACACCCGAAGAAAGACAAACAGCTTTCAAAAACATGATGGAAATTGCTTTAGGTGTTGCAAAATAA", "aa": "MTPHINAKEGDIAKTVLMPGDPLRAKFIAETFLDKGYRLVNTVRNMYMYTGTYKGRPVTIAGSGMGCPSIGIYSYELFKFYNVDNIIRIGTTGSYKKEIKLYETILATEAYADGASYRRLVLGDKSHVALPSAKLNKEIEKVAKNLNIKLTLGRVHSSDVFYSSVPLQQRIDETHALCVEMESYALFTNAEKLGKNAACLLTVSDNLITNDETTPEERQTAFKNMMEIALGVAK", "aa_hexdigest": "86abc929af10e3782e99a3cae902b30d", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C23D", "ncbi_nrp_id": "WP_013526885.1", "uniref100_id": "UniRef100_C4XEW4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C23D", "RefSeq:WP_013526885.1", "UniRef:UniRef100_C4XEW4" ] }, "ips": { "uniref100_id": "UniRef100_C4XEW4", "uniref90_id": "UniRef90_C4XEW4" }, "psc": { "uniref90_id": "UniRef90_C4XEW4", "gene": "deoD", "product": "purine-nucleoside phosphorylase", "ec_ids": [ "2.4.2.1" ], "uniref50_id": "UniRef50_S5MIP4", "kegg_orthology_id": "K03784", "go_ids": [ "GO:0004731", "GO:0006139" ] }, "pscc": { "uniref50_id": "UniRef50_S5MIP4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_S5MIP4" ], "product": "Uridine phosphorylase" }, "genes": [ "deoD" ], "id": "OAIKOHHJFB_861", "locus": "GCA000209735_00842" }, { "type": "cds", "sequence": "contig_1", "start": 471719, "stop": 472456, "strand": "+", "frame": 2, "gene": null, "product": "ATP-dependent 6-phosphofructokinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V6J2C4" ], "nt": "ATGAAAAAAATTAAGAGAATTGCTATTCTAACTTCAGGTGGCGATGCTCCTGGAATGAATAGTGCAATTAGAGCAGCAGCTAAAATGGCTCGTAGTCGTGGAATTGAAACATTTTTAGTATATGAAGGTTACAAAGGATTAGTTGATGATAATATTGTGCCTTCAACAAAAATTGATTTAGATTTCTTTAACAGTCGTGGAGGCACTTGTATTTATTCAGCAAGGTATCCTCAATTTAAAGATCCAGAAATTCGTGAAATTGCTATTAAAAATTTAAACAAACGTAAAATTGATGCTTTGATAGTTATTGGTGGTGATGGTTCTTATGCAGGAGCACAATTATTACATGAAAAAGGAATTAAAACTATTGGACTTCCAGGGACAATTGATAATGATATAGCTTCAAGTGATTTTACTATTGGCTATGATACAGCTTTAAATACAGTTGTTAGATGTATTGACCAAATTAGAGATACAGCAGCAAGTCATAAAAGAATTATGATTATTGAAGTTATGGGTAATAACTGTGGTGACTTAGCATTACACACAGGACTTGCAACAGGGGCAGAAGTTATTGCAACAAGTGAATACCGTCTTGATGAAAAAGAAATAGTTAAAACTTGTGTTGAATTAACTACAAATGCTAAACCTGTTAGAAGAAATATTACAATAGTTGTTAGTGAAAAATTATATAACATTAAACAACTAGCTAAAGACATTGAAAAAGCAACAGGATGA", "aa": "MKKIKRIAILTSGGDAPGMNSAIRAAAKMARSRGIETFLVYEGYKGLVDDNIVPSTKIDLDFFNSRGGTCIYSARYPQFKDPEIREIAIKNLNKRKIDALIVIGGDGSYAGAQLLHEKGIKTIGLPGTIDNDIASSDFTIGYDTALNTVVRCIDQIRDTAASHKRIMIIEVMGNNCGDLALHTGLATGAEVIATSEYRLDEKEIVKTCVELTTNAKPVRRNITIVVSEKLYNIKQLAKDIEKATG", "aa_hexdigest": "ce9d337683932a4658b53dc08bd059e9", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A975M207", "query_cov": 0.9795918367346939, "subject_cov": 0.8791208791208791, "identity": 0.5, "score": 233.0, "evalue": 1.75e-73, "valid": false, "product": "6-phosphofructokinase", "uniref50_id": "UniRef50_A0A7V6J2C4" }, "pscc": { "uniref50_id": "UniRef50_A0A7V6J2C4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V6J2C4" ], "product": "ATP-dependent 6-phosphofructokinase" }, "genes": [], "id": "OAIKOHHJFB_862", "locus": "GCA000209735_00843" }, { "type": "cds", "sequence": "contig_1", "start": 472580, "stop": 472711, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTATTGGAATTATTGATAATGATATTGTTGCTTCACCAATCTCAGAAGCATTAAGTATGATTAATAAATCTAAAGCTAAAGTTAGAAATAAAGCAATTAAATTTAATGAATTAAACCGTTTAGATTAA", "aa": "MAIGIIDNDIVASPISEALSMINKSKAKVRNKAIKFNELNRLD", "aa_hexdigest": "de38ff7d544e9981c8d979ad599baa0f", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4742.520399999999, "isoelectric_point": 9.397443580627442 }, "id": "OAIKOHHJFB_863", "locus": "GCA000209735_00844" }, { "type": "cds", "sequence": "contig_1", "start": 472938, "stop": 473264, "strand": "+", "frame": 3, "gene": null, "product": "HAD family hydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00210774DB" ], "nt": "ATGAGTCAAAAAGAAGATAAAAAACCAATAGTTTTTAGTGATGTTGATGGTACACTTTATGCTCGTGATTGTTATGTATCAAACTTCAATTTAAACATCATTAAACAAGATAATTTAAGTTTTAATATTGCTACTGGTAATCCAATTTGTCCAAGAATGTTAAAACTTGCAAAATTAGTAGATGCTGATTATATGATTGGATCTTCAGGTGTACAAATTTATGATTACAAACATAATAAATATTTACATGAAGAATATCTTAAAGCAAGCGTAGTTAAAAAGATTTTTAAACTCTTTAAAGAAAGCAAAGTTTCAGCTGCTGCTTGA", "aa": "MSQKEDKKPIVFSDVDGTLYARDCYVSNFNLNIIKQDNLSFNIATGNPICPRMLKLAKLVDADYMIGSSGVQIYDYKHNKYLHEEYLKASVVKKIFKLFKESKVSAAA", "aa_hexdigest": "f023992a6533a1e1c792cddc4a92b4ef", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00210774DB", "query_cov": 0.9074074074074074, "subject_cov": 0.875, "identity": 0.7240000000000001, "score": 147.0, "evalue": 8.7e-44, "valid": false, "product": "HAD family hydrolase", "uniref50_id": "UniRef50_UPI00210774DB" }, "pscc": { "uniref50_id": "UniRef50_UPI00210774DB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00210774DB" ], "product": "HAD family hydrolase" }, "genes": [], "id": "OAIKOHHJFB_864", "locus": "GCA000209735_00845" }, { "type": "cds", "sequence": "contig_1", "start": 473577, "stop": 473762, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGGTTATTGGTGACAGTGAAAATGATTATTCAATGTTCCACAAATTCAACTATTCTTATGTTATGGATAATGCTAAAGATGAAGTTAAAGATAAAGCTAACTTTATAACCAAAGCCGTTCAAGAACATGGTTTAGGTTATGCTGTTATGGATTATTTAGAAAAATTTAACAAGAGAAATAACTAG", "aa": "MVIGDSENDYSMFHKFNYSYVMDNAKDEVKDKANFITKAVQEHGLGYAVMDYLEKFNKRNN", "aa_hexdigest": "d61069b349d6d13fc53c2b8168a08c7c", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 48, "aa_cov": 0.7868852459016393, "hmm_cov": 1.0, "evalue": 1.1269964365025028e-15, "score": 54.09297561645508, "start": 1, "stop": 48 } ], "seq_stats": { "molecular_weight": 7174.945400000002, "isoelectric_point": 5.50738010406494 }, "id": "OAIKOHHJFB_865", "locus": "GCA000209735_00846" }, { "type": "cds", "sequence": "contig_1", "start": 474078, "stop": 474917, "strand": "+", "frame": 3, "gene": null, "product": "AAA domain-containing protein", "db_xrefs": [ "KEGG:K07133", "SO:0001217", "UniRef:UniRef50_C4XEW7", "UniRef:UniRef90_C4XEW7" ], "nt": "TTGTTAAAACTTGGAGTATTGAGTCAAAATATTATTCAAATAAATTTAGAACTTGAGGAAAATAAAAAACTAAGAAAAAGTCTTAATCTTTTAAACTTCATTAAAGAACAAATAATAAATGAAAACAAATATTATGTTTTAATCGATGAAATTCAAAATGTTAAAAATTTTGAAGATTCAGTTAATTATTTATTAAATATTAAAAATGTTGATCTTTATATTACTGGCAGTAATTCTAAATTTTTATCAAGAGATATTGAAACTCAAATTAGTAGTCGAGCTACAGAAATCAAAGTTAGACCATTAACATTCAAAGAATTTTACAGTGTTAAAAAAGAATTAAACATCGATGAAGTTTTTAAAGAATATTTGACTTTTGGTGGTATGCCTTTTTTAGTTAATTTAAATTATGAAAGCAAAAAGAAATATTTAAAAGAACTTTTTGAAACTACTTATATTAATGATATAGTTGAACGCAATAAAATAAAAAGTGATGAATACTTACAAGAATTTGTTGATTTTTTAACTTCTAATGTTGGTTCATTAACTAACAATTTAAATATCTGTAATACATATAAAACACTAAAACAAAACAATATAGCCTACTTTACTATTGATAAATATCTTAAAAATTTATCTCATACATATTTGATTGATTTAGTACAACTATTTAATTTAAAAGGAAAAAGAATAATCAAAAGATTAAACAAAATTTATTTTTATGACTTAGGAATTAGAAATTCAAGAATCGGCTACAACAATATTAAAATCGAACATGCAATTGAAAACATTGTTTACAATGAACTAGTTATAAGAAACTATGATGTAAATATTGGTTAA", "aa": "MLKLGVLSQNIIQINLELEENKKLRKSLNLLNFIKEQIINENKYYVLIDEIQNVKNFEDSVNYLLNIKNVDLYITGSNSKFLSRDIETQISSRATEIKVRPLTFKEFYSVKKELNIDEVFKEYLTFGGMPFLVNLNYESKKKYLKELFETTYINDIVERNKIKSDEYLQEFVDFLTSNVGSLTNNLNICNTYKTLKQNNIAYFTIDKYLKNLSHTYLIDLVQLFNLKGKRIIKRLNKIYFYDLGIRNSRIGYNNIKIEHAIENIVYNELVIRNYDVNIG", "aa_hexdigest": "089c8ef35cfe98923aeaf5bea060da5b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XEW7", "query_cov": 1.0, "subject_cov": 0.8353293413173652, "identity": 0.996, "score": 538.0, "evalue": 3.05e-192, "valid": true, "product": "AAA domain-containing protein", "uniref50_id": "UniRef50_C4XEW7", "kegg_orthology_id": "K07133" }, "pscc": { "uniref50_id": "UniRef50_C4XEW7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEW7" ] }, "genes": [], "id": "OAIKOHHJFB_866", "locus": "GCA000209735_00847" }, { "type": "cds", "sequence": "contig_1", "start": 475232, "stop": 476197, "strand": "+", "frame": 2, "gene": null, "product": "GTPase Obg", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q3XWL6" ], "nt": "ATGGCAAGATTTATTGATGAAATCAAAATAACTCTTCAAGCTGGTAAAGGTGGAGATGGCATGATCTCTTTCCGTCGTGAAGCTCATGTTGACAAAGGCGGACCAGATGGAGGAGATGGTGGAAGAGGCGGAAACATCTATTTTATAGGTGATTTAGGAAAAAACACTTTACTTAATTTATATGGAAATAAAAAGATAACTGCTGAAGATGGTATTAATGGCGGGCCTAAAAATCTTTATGGTGCAGCTGGCAAAGACACCTATGTTAAAGTTCCTCTTGGTACTGTTGTTTATAAAAATAATAAAGTGGTAGCTGATATTATTGAGCCTAATAAAAATTATTTAGTAGCTCAAGGTGGCCAAGGTGGCCGTGGCAATATGAAATTTAAAAGCCCTAGAAATACAGCTCCTAGAATTTGCGAAAATGGTACAAAAGGCGAAAAATTTGAAGCACATATTGTACTTAAAGTTATGTCGGATGTTGGTGTAGTTGGTAAACCTAGTGCAGGTAAAAGTACACTACTTTCAGTAATTAGTAACGCTAAAGCAAAAATTGCTGAATATGAATTTACAACCTTAGTTCCTCAATTGGGATTGGTTAGATATTTTGATAATTCATTTACTGTAGCTGACTTACCAGGATTAATTAAAGGTGCTAGTTTAGGTAAAGGTTTGGGTTTTCAATTTTTAAAACATATTGAAAGATGTCGTGTGATAGCTCATATTATTGACTTTGGTTTAAGTGAAAAAAATCCAATTGAAGACTATGAAACTATTAATAATGAATTAGCTTCATATAGCATGCATTTAGAAAATAAACCACAAATTGTGGTCGCTAATAAAAGTGATATGCCAGATTTTAAAGATCATCTTAAAGCGTTTAAAAAGAAATATCCTAAAGTTAAAGTTATAGTAATTAGTGCTTTAGAGCAAGAAAACCTTGATCAATTAAAAGCTGAGCTTTGA", "aa": "MARFIDEIKITLQAGKGGDGMISFRREAHVDKGGPDGGDGGRGGNIYFIGDLGKNTLLNLYGNKKITAEDGINGGPKNLYGAAGKDTYVKVPLGTVVYKNNKVVADIIEPNKNYLVAQGGQGGRGNMKFKSPRNTAPRICENGTKGEKFEAHIVLKVMSDVGVVGKPSAGKSTLLSVISNAKAKIAEYEFTTLVPQLGLVRYFDNSFTVADLPGLIKGASLGKGLGFQFLKHIERCRVIAHIIDFGLSEKNPIEDYETINNELASYSMHLENKPQIVVANKSDMPDFKDHLKAFKKKYPKVKVIVISALEQENLDQLKAEL", "aa_hexdigest": "e41655d052ef5ddce95ed7a8d8e7ce4d", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_Q3XWL6", "query_cov": 1.02803738317757, "subject_cov": 0.9041095890410958, "identity": 0.515, "score": 313.0, "evalue": 3.57e-102, "valid": false, "gene": "obgE", "product": "GTPase ObgE", "uniref50_id": "UniRef50_Q3XWL6", "go_ids": [ "GO:0003924" ] }, "pscc": { "uniref50_id": "UniRef50_Q3XWL6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q3XWL6" ], "product": "GTPase Obg" }, "genes": [], "id": "OAIKOHHJFB_867", "locus": "GCA000209735_00848" }, { "type": "cds", "sequence": "contig_1", "start": 476252, "stop": 476416, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09269.16" ], "nt": "ATGGTTGAAATTAAACTTGAAGATGATTTCACTATTGCAAATCCATATCGTGGTTTCTTTGAAGTTAGTGGGCCAAAAGTTGAAAAAATCTATGAAAAAATCCCTCTAATTTCTTATGATAATTTGATTAGATTTAATAATATGCTCAAAAAAATTGGTGTATGA", "aa": "MVEIKLEDDFTIANPYRGFFEVSGPKVEKIYEKIPLISYDNLIRFNNMLKKIGV", "aa_hexdigest": "ab4751304e8aae3c9754b8556ed596ba", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09269.16", "name": "DUF1967", "length": 46, "aa_cov": 0.8333333333333334, "hmm_cov": 1.0, "evalue": 1.456163488505088e-10, "score": 37.1574821472168, "start": 10, "stop": 54 } ], "seq_stats": { "molecular_weight": 6337.3634, "isoelectric_point": 6.147955513000488 }, "id": "OAIKOHHJFB_868", "locus": "GCA000209735_00849" }, { "type": "cds", "sequence": "contig_1", "start": 476596, "stop": 477063, "strand": "+", "frame": 1, "gene": null, "product": "Glucosamine-6-phosphate deaminase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEX0", "UniRef:UniRef90_C4XEX0" ], "nt": "ATGGCAATCAAATATTTAAGTATGAATAATTATTCTGATATTTGTGAATATGTTTCACAAGTTATTGAAAATCAGTTGTTTATGAAACCTAATTCAAATATAGCGATTAATGCAACTGATGATTTTAGAGGAATTTATAGTCAAATTGTTAATGATGTTAAAAAAGGAAAATACAGTTTTAATAAAGTTAATTTCTTTTTAACTGAAGAAATTGCAAATGTAACAAAAAATACAATTACTTCTGTTTATAGTTTCTTTGATAAGAATCTTTTAGCGCCAATTAACATGCCAACTAATCAAATTTTCTTTCCTATAACTTTCGCTCCAACAAAAGATCAACAATATGATCCCTTTATTTTCAAAAATAAAGCATTCGACTTGTCTATAGTGTTTTTGGGCAAAAACGGTGAGCTAGGTTATATCGATAAATTTAAGAATTACAACTCAGATTACACATCAAATTTTTGA", "aa": "MAIKYLSMNNYSDICEYVSQVIENQLFMKPNSNIAINATDDFRGIYSQIVNDVKKGKYSFNKVNFFLTEEIANVTKNTITSVYSFFDKNLLAPINMPTNQIFFPITFAPTKDQQYDPFIFKNKAFDLSIVFLGKNGELGYIDKFKNYNSDYTSNF", "aa_hexdigest": "fde3a6b565bab662ecd6ded12682939a", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 476578, "stop": 477333, "observations": { "mutation": [ 477061 ], "stop": [ 477061 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEX0", "query_cov": 1.0, "subject_cov": 0.6175298804780877, "identity": 1.0, "score": 314.0, "evalue": 4.61e-107, "gene_start": 1, "gene_end": 155, "reference_start": 7, "reference_end": 161, "reference_sequence": "MILRSPMAIKYLSMNNYSDICEYVSQVIENQLFMKPNSNIAINATDDFRGIYSQIVNDVKKGKYSFNKVNFFLTEEIANVTKNTITSVYSFFDKNLLAPINMPTNQIFFPITFAPTKDQQYDPFIFKNKAFDLSIVFLGKNGELGYIDKFKNYNSDYTSNFWLNNDLLNNFKIMQRNNQLNSLEIYSIGSGALFNSKKILVIALGLDKSNTIFKLMNTKTVNSKFAASMLIQHNDATLIADAPSGLAIKKN" }, "paralog": false, "identity": 0.9920318725099602, "score": 493.0, "evalue": 3.05e-180, "description": "Internal stop codon at 477061. Nonsense mutation around 477061.", "psc": { "uniref90_id": "UniRef90_C4XEX0", "product": "Glucosamine-6-phosphate deaminase", "uniref50_id": "UniRef50_C4XEX0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEX0", "UniRef:UniRef50_C4XEX0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEX0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEX0" ], "product": "Glucosamine-6-phosphate deaminase" } }, "id": "OAIKOHHJFB_869", "locus": "GCA000209735_00850" }, { "type": "cds", "sequence": "contig_1", "start": 477097, "stop": 477333, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGAGAAATAATCAACTTAATTCACTTGAAATTTATTCAATTGGTTCTGGTGCATTATTTAACTCGAAAAAGATTTTAGTTATTGCTTTAGGACTTGATAAAAGCAACACAATTTTTAAATTAATGAATACTAAAACAGTAAACTCTAAATTTGCAGCTTCAATGTTAATACAACATAATGATGCAACTTTAATTGCTGATGCTCCTTCGGGATTAGCTATTAAGAAAAACTAA", "aa": "MQRNNQLNSLEIYSIGSGALFNSKKILVIALGLDKSNTIFKLMNTKTVNSKFAASMLIQHNDATLIADAPSGLAIKKN", "aa_hexdigest": "8465db29de169fbeba7073fa9a33727a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8450.764899999995, "isoelectric_point": 9.901844215393066 }, "id": "OAIKOHHJFB_870", "locus": "GCA000209735_00851" }, { "type": "cds", "sequence": "contig_1", "start": 477367, "stop": 477717, "strand": "-", "frame": 2, "gene": null, "product": "Hypoxanthine phosphoribosyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PE13" ], "nt": "ATGACTACTTCTAGTTATGCAGGAGCAGGCAATAGTAGCGGTAGTGTTAAAATCATTATGGACTTGGCTCAAGATATTAGAAATAAAGATGTTTTAATTGTTGAAGACATTATTGATTCAGGAATTACACTTGATAAAATCAAGCAAATTCTTTTATCTCGTAAACCTCGTTCATTTAAAATAATAACTCTTTTAGACAAACCACACAACCGTAAAGTTGAATTGAAAGCTGATAAATCAGCCTTTATCGTGCCAAATGAATTCTTAGTTGGTTTTGGTTTGGATTATGATGAAAAAATGAGAAACCTCCCTTATATTGGAATTTTTGATAAAAAATATTTAAAAAAATAA", "aa": "MTTSSYAGAGNSSGSVKIIMDLAQDIRNKDVLIVEDIIDSGITLDKIKQILLSRKPRSFKIITLLDKPHNRKVELKADKSAFIVPNEFLVGFGLDYDEKMRNLPYIGIFDKKYLKK", "aa_hexdigest": "81af85f6aba0c8dde6570696b4e959be", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PE13", "query_cov": 0.9827586206896551, "subject_cov": 0.8085106382978723, "identity": 0.632, "score": 151.0, "evalue": 6.2e-45, "valid": false, "gene": "hpt", "product": "hypoxanthine phosphoribosyltransferase", "ec_ids": [ "2.4.2.8" ], "uniref50_id": "UniRef50_A0A3B0PE13", "kegg_orthology_id": "K00760", "go_ids": [ "GO:0006166" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PE13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PE13" ], "product": "Hypoxanthine phosphoribosyltransferase" }, "genes": [], "id": "OAIKOHHJFB_871", "locus": "GCA000209735_00852" }, { "type": "cds", "sequence": "contig_1", "start": 477835, "stop": 477924, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAGCAAAATATTGACTATCGAATTAAAAAGGTTTTATATACTCAAGAAGAAATTGAAGCTAAAATCAAAGAATTAGCAACTTGA", "aa": "MNKQNIDYRIKKVLYTQEEIEAKIKELAT", "aa_hexdigest": "53592a0486315c4d4995180bac52042d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3511.052100000001, "isoelectric_point": 8.064688682556149 }, "id": "OAIKOHHJFB_872", "locus": "GCA000209735_00853" }, { "type": "cds", "sequence": "contig_1", "start": 477917, "stop": 478450, "strand": "-", "frame": 1, "gene": null, "product": "Deoxynucleoside kinase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEX3", "UniRef:UniRef90_C4XEX2" ], "nt": "ATGTATGAAAAACAACCTAATATTCACATTGGTTTTCAATCATATATTATTGAAAGCTTATCAGACACATTTAAAAAAACAGTAGCTAAATTTAAAAATAATAACTTAAATTTTAGAGACAATCATATCTTTTTAGATCGTTTCAACATCGAACACTATGTTTTTGCTGTTGTCACTTTAATGGATAAAGATCCTAAATATCAAGCAGGTTTTGATGCTATGTTTGAAAAAATAGTTGATCCTGAAGATAATCCGGATTTGGCGATCTTTATTGATATTGATTTTAATGCATTCAAAGCTCGTTTATTAGCTCGAGGTCGCGCTTCAGAAGTTGATAATTATGCTCAAAATGAAACATACTTTAAATTACTTCATTCTTTATATAAAAAGCTTTATATAAGATTAGTTGAAAAATTTAAGATACCTTATGTTATCATTAATAGTAATAACAAAAGTGAAAAAGAAGTTTTACAAGAAGCTATTAATATAATAGATAGCTATGACTTCTCAAAATCAAAGAGGTATAATGAATAA", "aa": "MYEKQPNIHIGFQSYIIESLSDTFKKTVAKFKNNNLNFRDNHIFLDRFNIEHYVFAVVTLMDKDPKYQAGFDAMFEKIVDPEDNPDLAIFIDIDFNAFKARLLARGRASEVDNYAQNETYFKLLHSLYKKLYIRLVEKFKIPYVIINSNNKSEKEVLQEAINIIDSYDFSKSKRYNE", "aa_hexdigest": "f606b2530d97c1f8c64984f741358fc3", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 477917, "stop": 478600, "observations": { "mutation": [ 478453 ], "stop": [ 478453 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEX2", "query_cov": 1.0, "subject_cov": 0.7797356828193832, "identity": 1.0, "score": 350.0, "evalue": 2.36e-121, "gene_start": 1, "gene_end": 177, "reference_start": 51, "reference_end": 227, "reference_sequence": "MGLTMIIGISGMIGSGKSTLAKGLHKHYKKSVLLEEFQENDPVFNTFLKWMYEKQPNIHIGFQSYIIESLSDTFKKTVAKFKNNNLNFRDNHIFLDRFNIEHYVFAVVTLMDKDPKYQAGFDAMFEKIVDPEDNPDLAIFIDIDFNAFKARLLARGRASEVDNYAQNETYFKLLHSLYKKLYIRLVEKFKIPYVIINSNNKSEKEVLQEAINIIDSYDFSKSKRYNE" }, "paralog": false, "identity": 0.9911894273127754, "score": 451.0, "evalue": 2.33e-163, "description": "Internal stop codon at 478453. Nonsense mutation around 478453.", "psc": { "uniref90_id": "UniRef90_C4XEX2", "product": "Deoxynucleoside kinase domain-containing protein", "uniref50_id": "UniRef50_C4XEX3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEX2", "UniRef:UniRef50_C4XEX3" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEX3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEX3" ], "product": "Deoxynucleoside kinase domain-containing protein" } }, "id": "OAIKOHHJFB_873", "locus": "GCA000209735_00854" }, { "type": "cds", "sequence": "contig_1", "start": 478451, "stop": 478588, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATTGGTATAAGTGGCATGATTGGCAGTGGTAAAAGTACACTTGCTAAAGGCTTACATAAACATTATAAAAAATCAGTTTTATTAGAAGAATTTCAAGAAAATGACCCAGTTTTTAATACATTTTTAAAATGA", "aa": "MIIGISGMIGSGKSTLAKGLHKHYKKSVLLEEFQENDPVFNTFLK", "aa_hexdigest": "4738f28a360d814ddd5b12c268723004", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5007.825100000001, "isoelectric_point": 9.225441932678223 }, "id": "OAIKOHHJFB_874", "locus": "GCA000209735_00855" }, { "type": "cds", "sequence": "contig_1", "start": 478789, "stop": 479082, "strand": "-", "frame": 2, "gene": null, "product": "Deoxynucleoside kinase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEX3", "UniRef:UniRef90_C4XEX3" ], "nt": "GTGGAAAACCACACATCTAAATTAGCAGATTGTTTTAAAAAATTTAGCGAAAAAGGCTATAAATACAAGGATAATCACATATTTTTAGATCGTTTTAGTATTGAACATTACATTTTTGCTAATGTTAACTTAAGAACTAAAGGTAAAAAATATTTAGAAGGTTATGATGCACTTTTCTCTCATCTTATTACTAAAGATGAAACTCCAGATTTAGCAATTTTTCTAGATATGACTTTTGACACTTTCAAGAAAAGATTATTTGAAAGAGGTAGAGAAGTTGAAACTGAAAACTGA", "aa": "MENHTSKLADCFKKFSEKGYKYKDNHIFLDRFSIEHYIFANVNLRTKGKKYLEGYDALFSHLITKDETPDLAIFLDMTFDTFKKRLFERGREVETEN", "aa_hexdigest": "39cd8c50755ac4a863b55d0c19de2746", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 478597, "stop": 479289, "observations": { "mutation": [ 478791, 479133 ], "stop": [ 478791, 479133 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEX3", "query_cov": 1.0, "subject_cov": 0.4217391304347826, "identity": 0.99, "score": 202.0, "evalue": 3.92e-64, "gene_start": 1, "gene_end": 97, "reference_start": 70, "reference_end": 166, "reference_sequence": "MKKEVIIMVIGISGMISSGKSTLTRNLVKHYKNSMMLKEYEEDDDVFNTFLKWLYEKQPNLTIGFQSYVVENHTSKLADCFKKFSEKGYKYKDNHIFLDRFSIEHYIFANVNLRTKGKKYLEGYDALFSHLITKDETPDLAIFLDMTFDTFKKRLFERGREVETENWNKNKDYFRELYDLYKPLFIKQAKKYDLNYVIIETDNLTEKQVFEKAVEVINNYDFSKMERYNK" }, "paralog": false, "identity": 0.9869565217391304, "score": 458.0, "evalue": 1.53e-167, "description": "Internal stop codon at 478791, 479133. Nonsense mutation around 478791, 479133.", "psc": { "uniref90_id": "UniRef90_C4XEX3", "product": "Deoxynucleoside kinase domain-containing protein", "uniref50_id": "UniRef50_C4XEX3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEX3", "UniRef:UniRef50_C4XEX3" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEX3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEX3" ], "product": "Deoxynucleoside kinase domain-containing protein" } }, "id": "OAIKOHHJFB_875", "locus": "GCA000209735_00856" }, { "type": "cds", "sequence": "contig_1", "start": 479131, "stop": 479268, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATAGGTATAAGCGGAATGATTAGTAGTGGTAAAAGCACACTAACAAGAAATTTAGTTAAACACTACAAAAATTCAATGATGTTAAAAGAATATGAAGAGGACGATGATGTCTTCAATACATTCTTAAAATGA", "aa": "MVIGISGMISSGKSTLTRNLVKHYKNSMMLKEYEEDDDVFNTFLK", "aa_hexdigest": "ff5e553325210b21fc920c2e7d9c832b", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5171.940100000001, "isoelectric_point": 6.530084037780762 }, "id": "OAIKOHHJFB_876", "locus": "GCA000209735_00857" }, { "type": "cds", "sequence": "contig_1", "start": 479377, "stop": 479868, "strand": "-", "frame": 2, "gene": "greA", "product": "transcription elongation factor GreA", "db_xrefs": [ "GO:0003677", "GO:0032784", "GO:0070063", "RefSeq:WP_013354643.1", "SO:0001217", "UniParc:UPI0001A8C247", "UniRef:UniRef100_C4XEX4", "UniRef:UniRef50_A5IYH4", "UniRef:UniRef90_A0A3P8MDY7" ], "nt": "ATGGCAGGTAAAAAGCAAGAAAAAATCATATTAGCTAAAGAAACATTAGAAAAATATCAAGCAGAATATGAACACTTAATTAAAGTTGAAAGACCTGAAATTCAAGCAGCTTTAAAAGAAGCTAGAGCACAAGGTGACCTTTCAGAAAACGCTGAATATGATGCAGCCAGAGATCGTCAAGGTATTGTTGAAGCAAGAATTGTTGAACTTGAAGGCATTTTAGCAAATGCTGAAGTTATTGAAACAAACCAAAACAAAAAAGGTAAACTAAAAGCAGGTATCGGAGCAACAGTTAAATATCTTAACTTAAAAACAAATAAAGAAATTACAGTTAAAATCATGGGTATTCATGATTCTAATCCAATGGAAGGTAATATTTCTAATGAATCACCAGTAGCACAAGCTATCATGGAAGGTGATGAAGGCGCAACAGTTGAAGTTGATGTGCCTAATAAATACAGTATAAAAATCTTATCAATTCAATACAAATAG", "aa": "MAGKKQEKIILAKETLEKYQAEYEHLIKVERPEIQAALKEARAQGDLSENAEYDAARDRQGIVEARIVELEGILANAEVIETNQNKKGKLKAGIGATVKYLNLKTNKEITVKIMGIHDSNPMEGNISNESPVAQAIMEGDEGATVEVDVPNKYSIKILSIQYK", "aa_hexdigest": "9912c554e9a6fd621826c079a4171577", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001A8C247", "ncbi_nrp_id": "WP_013354643.1", "uniref100_id": "UniRef100_C4XEX4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C247", "RefSeq:WP_013354643.1", "UniRef:UniRef100_C4XEX4" ] }, "ips": { "uniref100_id": "UniRef100_C4XEX4", "uniref90_id": "UniRef90_A0A3P8MDY7" }, "psc": { "uniref90_id": "UniRef90_A0A3P8MDY7", "gene": "greA", "product": "transcription elongation factor GreA", "uniref50_id": "UniRef50_A5IYH4", "go_ids": [ "GO:0003677", "GO:0032784", "GO:0070063" ] }, "pscc": { "uniref50_id": "UniRef50_A5IYH4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IYH4" ], "product": "Transcription elongation factor GreA" }, "genes": [ "greA" ], "id": "OAIKOHHJFB_877", "locus": "GCA000209735_00858" }, { "type": "cds", "sequence": "contig_1", "start": 480386, "stop": 480619, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTTTTCAATTAATTCAACTGAAACAAATCAAGAATTATTTGACAAATATTATCCAATGTTAAGTGAAAAAGGGATGCTTGTTGTATTACAAAATTCAAATACAAAAAGTGGTTATAGAGTTTTAACTAAACATTTAAAACTTAATAAAATTGTTTATGAATTAAGTTATGTAAAAAGCGGATTCTTATATATAGTAAAAAGTAAAACTGAAATAAAAAATGAAAACTAA", "aa": "MIFSINSTETNQELFDKYYPMLSEKGMLVVLQNSNTKSGYRVLTKHLKLNKIVYELSYVKSGFLYIVKSKTEIKNEN", "aa_hexdigest": "dab3e62cf1061dcdea90814a94a25db2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9001.359299999998, "isoelectric_point": 9.363597679138184 }, "id": "OAIKOHHJFB_878", "locus": "GCA000209735_00859" }, { "type": "cds", "sequence": "contig_1", "start": 480713, "stop": 481114, "strand": "-", "frame": 1, "gene": null, "product": "Methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEX5", "UniRef:UniRef90_C4XEX5" ], "nt": "ATGATTAGTGCTGTAATCAATTTAAGTAAAGGTGCAAAAATTGGCTTAATTGTTTCTGCTATTGTTTTTGTGATTATTGCCTTTATAACTTATATTATTTTACTAGTTAAAACTAGAAAAATTCGTAATGAAATAAATACCAAAGAAGCTCAAAAAGCTCATGAAGCTATTTTGAAAATCAGAGGTGAAGAATTAGGAGAATTCCCTAAAGAATTAAAAGAATTTTTTAAAAGTAATCTTGATGATTATGATTTAGAAAACTTTATTAATACTATTTATTTAAATGATGTTCAAGAGATTTTACTTGTTGGAAGTAAGTTAGAATATCCAACAGCTTTATTTAAAAATAAATCACAAGCTCAAATTTGTTTAGAAAAACAAAACATGAATGTAAGTTTATGA", "aa": "MISAVINLSKGAKIGLIVSAIVFVIIAFITYIILLVKTRKIRNEINTKEAQKAHEAILKIRGEELGEFPKELKEFFKSNLDDYDLENFINTIYLNDVQEILLVGSKLEYPTALFKNKSQAQICLEKQNMNVSL", "aa_hexdigest": "572804c1c177a7f3bcdaabb328ea76d9", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 480410, "stop": 481114, "observations": { "mutation": [ 480715 ], "stop": [ 480715 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEX5", "query_cov": 1.0, "subject_cov": 0.5495867768595041, "identity": 0.992, "score": 242.0, "evalue": 2.79e-79, "gene_start": 1, "gene_end": 133, "reference_start": 1, "reference_end": 133, "reference_sequence": "MISAVINLSKGAKIGLIVSAIVFVIIAFITYIILLVKTRKIRNEINTKEAQKAHEAILKIRGEELGEFPKELKEFFKSNLDDYDLENFINTIYLNDVQEILLVGSKLEYPTALFKNKSQAQICLEKQKMNVSLWNKAVLELPKYFKDQPSFINVNELKTQDKKFKLIFSINSTETNQELFDKYYPMLSEKGMLVVLQNSNTKSGYRVLTKHLKLNKIVYELSYVKSGFLYIVKSKTEIKNEN" }, "paralog": false, "identity": 0.9871794871794872, "score": 445.0, "evalue": 1.44e-161, "description": "Internal stop codon at 480715. Nonsense mutation around 480715.", "psc": { "uniref90_id": "UniRef90_C4XEX5", "product": "Methyltransferase", "uniref50_id": "UniRef50_C4XEX5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEX5", "UniRef:UniRef50_C4XEX5" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEX5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEX5" ], "product": "Methyltransferase" } }, "id": "OAIKOHHJFB_879", "locus": "GCA000209735_00860" }, { "type": "cds", "sequence": "contig_1", "start": 481107, "stop": 481541, "strand": "-", "frame": 3, "gene": "ruvX", "product": "Holliday junction resolvase RuvX", "db_xrefs": [ "EC:3.1.21.10", "GO:0006364", "RefSeq:WP_013354645.1", "SO:0001217", "UniParc:UPI0001E330FB", "UniRef:UniRef100_A0AB32XC51", "UniRef:UniRef50_A5IYK4", "UniRef:UniRef90_A0AB32XC51" ], "nt": "ATGCGAGCTTTAGCTCTAGATTTAGGCACTAAAACTTGCGGTTTTGCAATTAGTGATGAAGGCATGATTATAGCTAGTGCACTTGAAACATTACATTTTGAAGAAAATGATTTTAATGCAATTATTGAGCAAATCAAAATTTACCAAAAAACATATAAAATAGATAAATTGGTATTAGGAAAGCCTTTAAGATCTAATAATACAAGTAGTGAAAGAACAGTGCTTTTTGAATCTTTTGCTCATAAACTAAAAGGTATTTTTAAAGACTACAAAATTTATCAAGTTAATGAATATGGAACTACTATAGCTGCTACTAATATTTTAAAAGAAGCAAAACTTTCTATTAAAAAAAGAAAAGCTCATAAAGATACTTTGTCAGCTGTTTTAATCTTGCAAGAATTTTTAAATTATGGAGGTGTAGAATTACATGATTAG", "aa": "MRALALDLGTKTCGFAISDEGMIIASALETLHFEENDFNAIIEQIKIYQKTYKIDKLVLGKPLRSNNTSSERTVLFESFAHKLKGIFKDYKIYQVNEYGTTIAATNILKEAKLSIKKRKAHKDTLSAVLILQEFLNYGGVELHD", "aa_hexdigest": "e8fd5b429388892961df9e96ae9ae147", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001E330FB", "ncbi_nrp_id": "WP_013354645.1", "uniref100_id": "UniRef100_A0AB32XC51", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330FB", "RefSeq:WP_013354645.1", "UniRef:UniRef100_A0AB32XC51" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XC51", "uniref90_id": "UniRef90_A0AB32XC51" }, "psc": { "uniref90_id": "UniRef90_A0AB32XC51", "gene": "ruvX", "product": "Holliday junction resolvase RuvX", "ec_ids": [ "3.1.21.10" ], "uniref50_id": "UniRef50_A5IYK4", "go_ids": [ "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_A5IYK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IYK4" ], "product": "Putative pre-16S rRNA nuclease" }, "genes": [ "ruvX" ], "id": "OAIKOHHJFB_880", "locus": "GCA000209735_00861" }, { "type": "cds", "sequence": "contig_1", "start": 481541, "stop": 482266, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02272.25", "PFAM:PF07973.19" ], "nt": "ATGGGCGCAATTATGACTATTGACGAAAGTGAATACATGGATCCTAATAACATTCGTATTGTTGACTTTAAAGGTATTACAAAAGACCTTTGCGGTGGTACTCACTTAGATAGAACCGGAATTTTAGAAGACTTTAAAATTACTAAAGTTGAGAAAAAAACTGCTGGTGTATTTAGAATAAGAGCTGTAGCTTCTCATAAATATGTTAAACAATATTTAACAGAATTAAATCAAAGTTTAAAAGAACAATTAGATGCTTTAGTTAAAAAAGCTCAAAGCTTTGATAGTAAATATCAAATTAAATTTACACCTAATAAAGATGTTGAAATTGAATGTGCAAACATTAAAAAAACAATTGATAAAGTTCATGAAGATATCAAAGAATTAATCAAAAACAAAAGCAATATAACCTTTGATTTTGATAGTGTTCAATTAACTAAAAACAAAAAATACCCTTATTATTTAAACATTGATTTTGATAGTTCCCAAGTTAAAGTTGCTGCTGCAACGCTTAGAGAAAAATTTCCTGAAGCTATAATTGTTTTAGGTGCTAAAAGTAATAATCAAATTCTTTTATGTGTGGCCGCTAAAGTGCTTGATGCTAACAAATTATTCCAACAAATAGCTTCAAAAACCAATGGTCGTGGTGGTGGAAGTTCAATTATTTCAATGGGTAAAGTTGACTATACTAAAGATCTTGAAAATATTATTAAGGAACTTATTTAA", "aa": "MGAIMTIDESEYMDPNNIRIVDFKGITKDLCGGTHLDRTGILEDFKITKVEKKTAGVFRIRAVASHKYVKQYLTELNQSLKEQLDALVKKAQSFDSKYQIKFTPNKDVEIECANIKKTIDKVHEDIKELIKNKSNITFDFDSVQLTKNKKYPYYLNIDFDSSQVKVAAATLREKFPEAIIVLGAKSNNQILLCVAAKVLDANKLFQQIASKTNGRGGGSSIISMGKVDYTKDLENIIKELI", "aa_hexdigest": "7d29e705574c7529a619c84fc12dbbe1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02272.25", "name": "DHHA1", "length": 89, "aa_cov": 0.35269709543568467, "hmm_cov": 0.9775280898876404, "evalue": 2.2973127545926596e-09, "score": 33.768638610839844, "start": 156, "stop": 240 }, { "id": "PF07973.19", "name": "tRNA_SAD", "length": 43, "aa_cov": 0.17842323651452283, "hmm_cov": 1.0, "evalue": 1.048758146089775e-13, "score": 47.12787628173828, "start": 18, "stop": 60 } ], "seq_stats": { "molecular_weight": 27148.053900000024, "isoelectric_point": 8.986135292053223 }, "id": "OAIKOHHJFB_881", "locus": "GCA000209735_00862" }, { "type": "cds", "sequence": "contig_1", "start": 482324, "stop": 483457, "strand": "-", "frame": 1, "gene": "alaS", "product": "alanine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.7", "GO:0000166", "GO:0004813", "GO:0005737", "GO:0006419", "SO:0001217", "UniRef:UniRef50_Q98Q12", "UniRef:UniRef90_C4XEX7" ], "nt": "ATGCAAGATGCTCCAACTAACTATGACAGCGATCTTTTTATTAACATAATTAAAGAAATAGAAAAATATGCCACAGTTAAATATGACATTGATAACTACTTTAAAAAAGATAAAAAGCAAACTGAAATCAACACTAACTTTAAAGTTATAGCAGACCACATTAGAACAGTGGTTAATGCTATAGCTGATGGAGCTAAAGTTTCAAATGTTGGCCGTGGTTACATTATTAGAAGACTTATTCGACGTTCAATTTACAAAGGAATGCAACTAGGAGTTAAAGGTCTATTTTTACATAAATTAGTAGATGTAGTACAAGATTCATTACCTTATGAATATGACAAAAAACCTGTTGTAGTAGCCATTAAAGAAGAAGAATTAGCTTTCCATCAAACTATTGAAAAAGGTAAATTATTACTTGAAAAATTTATGGATAATAAAACTAAAATTTTCTCAGGTGAAGATGCTTTTAATTTACTTGAAACTTATGGCTTTCCAATTGAGTTAACTGTTGAAATTTTAGCTAAGAAAAATATTAAAGTTGATCTTAAAGCTTTTGAAGAAGCTAAGGAAAGACACGCCAACTTAAGCAGGGGTGAAAAAGTTAACGGAATGGATAAAGTAATTAACTCATTAGCCTTAATTAAAGGCAAAATCGGTGAATTTATTGGTTATACAGACTTAGAAAGTAAATCAAAAATTCTTAAACTTTTAAATAATGAGGAAGAGGTAAAATCATTAAATGGTGAAGGTTATTTGATCTTAGATAAAACACCTTTTTATGCTACTAGTGGTGGTCAAAATCATGACCATGGTTTCATTTTACAAGGTAAAAATAAAATCAAAATTTTAGATGTTTTTAAAGACAAATTTGGTAACCATATTCATAAAATTGAAGGCAAAATTAACAATAAGGATACTGTCGAATGTTATGTTGATAAAGAAGTAAGAATTGGTATGGCTCGAGGTCACTCAGCAACTCACTTATTATTCCGTGCTTTAGTTAAAGTACTTGGACCTCATATTGATCAACTTGGTTCAGATATCACCGAAGAACGTTTAATATTTGACTTTCCAGCTGATGAAAAACCAACTGATGAACAAATTAAAAAAATCGAAGACATAATGCATGGTTGA", "aa": "MQDAPTNYDSDLFINIIKEIEKYATVKYDIDNYFKKDKKQTEINTNFKVIADHIRTVVNAIADGAKVSNVGRGYIIRRLIRRSIYKGMQLGVKGLFLHKLVDVVQDSLPYEYDKKPVVVAIKEEELAFHQTIEKGKLLLEKFMDNKTKIFSGEDAFNLLETYGFPIELTVEILAKKNIKVDLKAFEEAKERHANLSRGEKVNGMDKVINSLALIKGKIGEFIGYTDLESKSKILKLLNNEEEVKSLNGEGYLILDKTPFYATSGGQNHDHGFILQGKNKIKILDVFKDKFGNHIHKIEGKINNKDTVECYVDKEVRIGMARGHSATHLLFRALVKVLGPHIDQLGSDITEERLIFDFPADEKPTDEQIKKIEDIMHG", "aa_hexdigest": "ac67aa2f7429c6039832759aba239c3a", "start_type": "ATG", "rbs_motif": null, "genes": [ "alaS" ], "truncated": "both", "pseudogene": { "start": 482021, "stop": 483757, "observations": { "mutation": [ 482326, 483565, 483691, 483751 ], "stop": [ 482326, 483565, 483691, 483751 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEX7", "query_cov": 1.0, "subject_cov": 0.4308571428571429, "identity": 1.0, "score": 744.0, "evalue": 1.2e-263, "gene_start": 1, "gene_end": 377, "reference_start": 238, "reference_end": 614, "reference_sequence": "MTMEKLTSKEIREKWLRYFESKGHLRVESKSLIPVNDPSLLWINSGVATLKDYFSGKKKPPRNRLTNSQKAIRTNDIENVGVTSRHHTFFEMLGNFSIGDYFKKEAIAFGYEFLTNELQIDPKKLYMTYFAEDLDTKKYWMEQGVEESHLIPGTRDTNFWDVGSGPCGPDTEIFYDRGPKYNKRGLELLKKDIENDRYIEIWNIVFSQFNNDGENNYTELKQKNIDTGAGLERIASIMQDAPTNYDSDLFINIIKEIEKYATVKYDIDNYFKKDKKQTEINTNFKVIADHIRTVVNAIADGAKVSNVGRGYIIRRLIRRSIYKGMQLGVKGLFLHKLVDVVQDSLPYEYDKKPVVVAIKEEELAFHQTIEKGKLLLEKFMDNKTKIFSGEDAFNLLETYGFPIELTVEILAKKNIKVDLKAFEEAKERHANLSRGEKVNGMDKVINSLALIKGKIGEFIGYTDLESKSKILKLLNNEEEVKSLNGEGYLILDKTPFYATSGGQNHDHGFILQGKNKIKILDVFKDKFGNHIHKIEGKINNKDTVECYVDKEVRIGMARGHSATHLLFRALVKVLGPHIDQLGSDITEERLIFDFPADEKPTDEQIKKIEDIMHGWIKQNVEREYIVTNIKKAKEMGAIMTIDESEYMDPNNIRIVDFKGITKDLCGGTHLDRTGILEDFKITKVEKKTAGVFRIRAVASHKYVKQYLTELNQSLKEQLDALVKKAQSFDSKYQIKFTPNKDVEIECANIKKTIDKVHEDIKELIKNKSNITFDFDSVQLTKNKKYPYYLNIDFDSSQVKVAAATLREKFPEAIIVLGAKSNNQILLCVAAKVLDANKLFQQIASKTNGRGGGSSIISMGKVDYTKDLENIIKELI" }, "paralog": false, "identity": 0.9930795847750865, "score": 1137.0, "evalue": 0.0, "description": "Internal stop codon at 482326, 483565, 483691, 483751. Nonsense mutation around 482326, 483565, 483691, 483751.", "psc": { "uniref90_id": "UniRef90_C4XEX7", "gene": "alaS", "product": "alanine--tRNA ligase", "ec_ids": [ "6.1.1.7" ], "uniref50_id": "UniRef50_Q98Q12", "go_ids": [ "GO:0000166", "GO:0004813", "GO:0005737", "GO:0006419" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEX7", "EC:6.1.1.7", "UniRef:UniRef50_Q98Q12", "GO:0000166", "GO:0004813", "GO:0005737", "GO:0006419" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q12", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q12" ], "product": "Alanine--tRNA ligase" } }, "id": "OAIKOHHJFB_882", "locus": "GCA000209735_00863" }, { "type": "cds", "sequence": "contig_1", "start": 483749, "stop": 483895, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGGTAACTTTTCAATTGGCGATTACTTTAAAAAAGAAGCTATCGCTTTTGGTTATGAATTCTTAACTAATGAATTACAAATAGACCCTAAAAAACTTTACATGACTTATTTTGCAGAAGATTTGGATACTAAAAAATACTGA", "aa": "MLGNFSIGDYFKKEAIAFGYEFLTNELQIDPKKLYMTYFAEDLDTKKY", "aa_hexdigest": "ad41bcfa2a8b765b721ac15ac798e365", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5711.468700000001, "isoelectric_point": 4.7658586502075195 }, "id": "OAIKOHHJFB_883", "locus": "GCA000209735_00864" }, { "type": "cds", "sequence": "contig_1", "start": 484172, "stop": 484891, "strand": "-", "frame": 1, "gene": "mnmA", "product": "tRNA 2-thiouridine(34) synthase MnmA", "db_xrefs": [ "EC:2.8.1.13", "GO:0008033", "GO:0016740", "GO:0016783", "KEGG:K00566", "SO:0001217", "UniRef:UniRef50_Q600M2", "UniRef:UniRef90_C4XEX8" ], "nt": "ATGGGTCATTATGCCAAAGTTAAGAATGGTAAACTTTATCGGGCGAAAGATCAAAATAAAGATCAAACATATTTTTTAGCTCAATTAACTAATCAACAACTAGAAAAAGTTTTAATGCCTCTTGCTGATTATCCTAAAGAAGAAATAAGAAAAATAGCTAAAAAATTAGACTTAGCTACAGCTACAAAAAAAGATTCAACAGGTATTTGTTTTATTGGCGAAAGACATTTTACAAAATTCTTACAAAACTATATTCCAGCTCAAAAAGGAAAAATATTAGACATTAAAACTAAAGAAGTTTTAGGAACTCATGATGGTTGTTTCTATTACACTATCGGTCAAAGAAAAGGATTAAATTTAGGCGGCAAAAGTGAAGCTCACTATGTTTGCGGTCATGATGTCAAAAACAATATTATTTATGCTGCACCAGCTAGTGATCCTAGTTATTTAGAAAGTAATATGCTAATAGCTTCAAACTTATGTCTAAATCATCATCGCTTTAATAAAAACAAATTAAGTGCTAAATTTAGATATCGTCAAAAAGATCTTAAAGTTAAAGTTGAATTCTTAGAAAATAATAAAATTAAAGTATTTTATCCATCAAAAGCTCAAGCAGTAACCCCCGGTCAACAAGTTGTTTTATATGATCAAGACAAGTGCTTAGGCGGAGCAATAATTGAAGAAATATACTTAAACAATATTAAAAAAGATTACATTTAG", "aa": "MGHYAKVKNGKLYRAKDQNKDQTYFLAQLTNQQLEKVLMPLADYPKEEIRKIAKKLDLATATKKDSTGICFIGERHFTKFLQNYIPAQKGKILDIKTKEVLGTHDGCFYYTIGQRKGLNLGGKSEAHYVCGHDVKNNIIYAAPASDPSYLESNMLIASNLCLNHHRFNKNKLSAKFRYRQKDLKVKVEFLENNKIKVFYPSKAQAVTPGQQVVLYDQDKCLGGAIIEEIYLNNIKKDYI", "aa_hexdigest": "e6786ae432ac4a22986b42ab2e220612", "start_type": "ATG", "rbs_motif": null, "genes": [ "mnmA" ], "truncated": "3-prime", "pseudogene": { "start": 484172, "stop": 485191, "observations": { "mutation": [ 485032, 485179 ], "stop": [ 485032, 485179 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEX8", "query_cov": 1.0, "subject_cov": 0.6256544502617801, "identity": 1.0, "score": 477.0, "evalue": 3.9e-168, "gene_start": 1, "gene_end": 239, "reference_start": 144, "reference_end": 382, "reference_sequence": "MKLIFNIRINMHKKKVILGMSGGVDSSVAAYLLLQQGYDVEGLFMRNWDSYVNNDFLGNENISQDICPQEQDYQDALAVANKLGIKLHRVDFVKEYWDNVFENFIEEYKKGRTPNPDILCNKYIKFDAFLKYAFEKLNADYIAMGHYAKVKNGKLYRAKDQNKDQTYFLAQLTNQQLEKVLMPLADYPKEEIRKIAKKLDLATATKKDSTGICFIGERHFTKFLQNYIPAQKGKILDIKTKEVLGTHDGCFYYTIGQRKGLNLGGKSEAHYVCGHDVKNNIIYAAPASDPSYLESNMLIASNLCLNHHRFNKNKLSAKFRYRQKDLKVKVEFLENNKIKVFYPSKAQAVTPGQQVVLYDQDKCLGGAIIEEIYLNNIKKDYI" }, "paralog": false, "identity": 0.9941002949852508, "score": 687.0, "evalue": 1.82e-253, "description": "Internal stop codon at 485032, 485179. Nonsense mutation around 485032, 485179.", "psc": { "uniref90_id": "UniRef90_C4XEX8", "gene": "mnmA", "product": "tRNA 2-thiouridine(34) synthase MnmA", "ec_ids": [ "2.8.1.13" ], "uniref50_id": "UniRef50_Q600M2", "kegg_orthology_id": "K00566", "go_ids": [ "GO:0008033", "GO:0016740", "GO:0016783" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEX8", "EC:2.8.1.13", "UniRef:UniRef50_Q600M2", "KEGG:K00566", "GO:0008033", "GO:0016740", "GO:0016783" ] }, "pscc": { "uniref50_id": "UniRef50_Q600M2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q600M2" ], "product": "tRNA-specific 2-thiouridylase MnmA" } }, "id": "OAIKOHHJFB_884", "locus": "GCA000209735_00865" }, { "type": "cds", "sequence": "contig_1", "start": 485177, "stop": 485290, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02540.23", "PFAM:PF03054.22" ], "nt": "ATGCATAAAAAGAAAGTTATTTTAGGAATGTCTGGAGGAGTAGACTCTTCAGTAGCTGCATATTTATTACTTCAACAAGGTTATGATGTTGAAGGTCTTTTTATGCGGAATTGA", "aa": "MHKKKVILGMSGGVDSSVAAYLLLQQGYDVEGLFMRN", "aa_hexdigest": "bb6bd5eed5ff4427b49b46d59b44511e", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02540.23", "name": "NAD_synthase", "length": 22, "aa_cov": 0.5945945945945946, "hmm_cov": 1.0, "evalue": 1.6459041939253977e-07, "score": 26.732755661010742, "start": 2, "stop": 23 }, { "id": "PF03054.22", "name": "tRNA_Me_trans", "length": 34, "aa_cov": 0.918918918918919, "hmm_cov": 1.0, "evalue": 7.045166264792815e-13, "score": 44.75435256958008, "start": 4, "stop": 37 } ], "seq_stats": { "molecular_weight": 4056.7289999999994, "isoelectric_point": 8.160811042785646 }, "id": "OAIKOHHJFB_885", "locus": "GCA000209735_00866" }, { "type": "cds", "sequence": "contig_1", "start": 485982, "stop": 486071, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTCTTTAACTATTGCTCGTGCTGTTAATATTGTTTTAGAAAACGGATTAAGACTTTTAGGTGTTAGTTCAAAACACAAAATGTAA", "aa": "MNSLTIARAVNIVLENGLRLLGVSSKHKM", "aa_hexdigest": "10bc86e11d2336a6882e93b0147c64d7", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3165.7748, "isoelectric_point": 11.000127601623536 }, "id": "OAIKOHHJFB_886", "locus": "GCA000209735_00867" }, { "type": "cds", "sequence": "contig_1", "start": 486111, "stop": 486296, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05746.21" ], "nt": "TTGCTTAAGAAATCTAAATTAGATGTTTCAAAACTTAAAGCAACAAATATTGACAACGAACTTGATAAAAAATTAGTTTCGACATTGCTTGAATTTCCAGAAGTTATCATTAGTTCAGCAACTAAGTACTTAACACACTTACTAACACAATACTTAATTAGAGTTGCCAAAGAATTTAACTCATGA", "aa": "MLKKSKLDVSKLKATNIDNELDKKLVSTLLEFPEVIISSATKYLTHLLTQYLIRVAKEFNS", "aa_hexdigest": "67f166eb88701eb9e028287d6f393c8a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05746.21", "name": "DALR_1", "length": 62, "aa_cov": 0.9836065573770492, "hmm_cov": 1.0, "evalue": 3.0179079662843084e-10, "score": 36.31890106201172, "start": 2, "stop": 61 } ], "seq_stats": { "molecular_weight": 6981.156399999999, "isoelectric_point": 9.34754505157471 }, "id": "OAIKOHHJFB_887", "locus": "GCA000209735_00868" }, { "type": "cds", "sequence": "contig_1", "start": 486459, "stop": 486587, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATAGCAATGCAAGACTTAGGTTACGACCCAAACAAATTAATTGTTCTTTGTTTACAATTAGTAAGACTTATGAAAGATGGTCAAGAATACAAAATGTCAAAACGTAAAGGTACAAGTTTTTGA", "aa": "MKIAMQDLGYDPNKLIVLCLQLVRLMKDGQEYKMSKRKGTSF", "aa_hexdigest": "c6255dd01225134f784e6a40e94c48ce", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4891.8623, "isoelectric_point": 9.579889106750485 }, "id": "OAIKOHHJFB_888", "locus": "GCA000209735_00869" }, { "type": "cds", "sequence": "contig_1", "start": 487017, "stop": 487610, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03485.21" ], "nt": "ATGGATAAAAACATTATTAAAAGCAAAATTATTAAAGAATTAAAACCAATTTTAAACAAATTAGGGATTGAAAAGGACGATCTCTTAATTGAACCTAAAGTCAAAGCCGATTTCGCTTCTAACGTTGCATTAGGAAACAAAGGCAAAAATCCTATGGAACTTGCCAAAAAAATTGTTAAAGAATTAGAGCCTAAACTAACTTCATTAGGTCTAAAAAGTGTAGAAATTGCAGCTCCTGGATTTTTAAACTTTTACATTGATGATCAAACAATTATTAAAAGTTTGAACCAAATTATCAAACAAAATGAAAACTTTGGTCGTGGTAAATTAAAAGGAAATCTAAACATCGAATGGGTTTCAGCAAACCCTACTGGATACTTGCACATTGGTCATGCACGTAATGCTGCTATTGGATCAGCACTATACAACATTTGTACTTTCGCAGGTTTAAATGTTTTACGTGAATATTACATCAATGATGGTGGTAACCAAATTAACACTTTAGCTCAAAGTATATTTGCAAGATATAAACAATTTTATGAACCAGATTTCCCTATGCCAGAAGACTGCTATGGAGGTCAAGACATTGTTTGA", "aa": "MDKNIIKSKIIKELKPILNKLGIEKDDLLIEPKVKADFASNVALGNKGKNPMELAKKIVKELEPKLTSLGLKSVEIAAPGFLNFYIDDQTIIKSLNQIIKQNENFGRGKLKGNLNIEWVSANPTGYLHIGHARNAAIGSALYNICTFAGLNVLREYYINDGGNQINTLAQSIFARYKQFYEPDFPMPEDCYGGQDIV", "aa_hexdigest": "34b945c66ddadab51a07dedeb599802f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03485.21", "name": "Arg_tRNA_synt_N", "length": 87, "aa_cov": 0.41116751269035534, "hmm_cov": 0.9540229885057471, "evalue": 2.6087763727996026e-13, "score": 46.527488708496094, "start": 6, "stop": 86 } ], "seq_stats": { "molecular_weight": 21959.130199999992, "isoelectric_point": 8.692804145812985 }, "id": "OAIKOHHJFB_889", "locus": "GCA000209735_00870" }, { "type": "cds", "sequence": "contig_1", "start": 487882, "stop": 488217, "strand": "-", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEY0", "UniRef:UniRef90_C4XEY0" ], "nt": "ATGTTTAAGAAAAAACTATTAATATCAAGTTTAGCAGTTGCTTCATTTTTACCTGTATCAGTTGTTTGTGTCTCTTGTGGAGATGGTAAAACTGATGAAGAAAAATACTTAGAAGCTGCTAAAGTTAAATTTGAAAACATTAAAGCATATTTAGCCAAAATAAATGACTCTTATGAAGAGTATCAAAAAACTACAAAAATAACTGATTCTGCAATTATTGCTAAAAGAGATAAAGCTAAAAAACTTGGCGAAAAAATGAAATCTGAAAATTACTACAGCGAATTAATTAAAGACAAAAAAATAATAGAAATAAATACTATTTTAAATGACTTATGA", "aa": "MFKKKLLISSLAVASFLPVSVVCVSCGDGKTDEEKYLEAAKVKFENIKAYLAKINDSYEEYQKTTKITDSAIIAKRDKAKKLGEKMKSENYYSELIKDKKIIEINTILNDL", "aa_hexdigest": "72eec781810d4f72e02ba356db2a85c2", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_C4XEY0", "query_cov": 1.0, "subject_cov": 0.8740157480314961, "identity": 1.0, "score": 208.0, "evalue": 9.2e-68, "valid": true, "product": "Lipoprotein", "uniref50_id": "UniRef50_C4XEY0" }, "pscc": { "uniref50_id": "UniRef50_C4XEY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEY0" ], "product": "Lipoprotein" }, "genes": [], "id": "OAIKOHHJFB_890", "locus": "GCA000209735_00871" }, { "type": "cds", "sequence": "contig_1", "start": 488234, "stop": 488566, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTTACTTGCAGGCGTTAACAAAGATTATGTTGACTATTCAGTAGACAGTAGTCTAGGAAATGCAACACTTGAAGAATATGTAAAAGCTATTGATGCTTCAAGAGCTGAAATGTACAAAAACTTAATTATTTCTAGAGTTGCATTAGTATGTGTATTAGGAATATTTGCAATGTTAACATTCCTTCCTGCAGTTATTGAAGATGCAATTAACAAGAAAAAATATGGTTCTTTAGAAACTTATGAAGAATACAAAGCACAAAAATTAGCAGAATTGTCAAAAGAAGAAGCTACTTTAACTAAACAAAAACCAGTTGAAGTTCAAGCTTAA", "aa": "MFLLAGVNKDYVDYSVDSSLGNATLEEYVKAIDASRAEMYKNLIISRVALVCVLGIFAMLTFLPAVIEDAINKKKYGSLETYEEYKAQKLAELSKEEATLTKQKPVEVQA", "aa_hexdigest": "25fdb73ff790f550619a8d5400bec70e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12260.048999999988, "isoelectric_point": 4.990429496765136 }, "id": "OAIKOHHJFB_891", "locus": "GCA000209735_00872" }, { "type": "cds", "sequence": "contig_1", "start": 489002, "stop": 489394, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTGCTCCAGATCAAACATTTGCAGAAGCAATTAAATCAGGTAAAAGTGTTACTGCTTTACCTAAATTAGGAGCTGGTCTTGGTGTATTCCTTGCTGTTGCTGCTATCTTCTTTGCTTATGATGGATTCTATGTCGCTGCTGGAATTCAAAGTGAAATGAAAGAACCTAAAAAGACTCCTTTAGCAATTTTCATTGGTTTAGGAATCACAACCTTAATTTACTTAATTGTTGCTATTTCAATGTCACTTAACGGTGGATCATTCTTTGGCTTCAATGGATACATGAGTAGTTTATTCAAAAATGAAAAAGCTGGTCGAATTGTTTTTGGTTTAATTAACATTTGTATTTCAATTGGTGTTCTAGGTATTATTAATGGTTTCTCAATGTGA", "aa": "MPAPDQTFAEAIKSGKSVTALPKLGAGLGVFLAVAAIFFAYDGFYVAAGIQSEMKEPKKTPLAIFIGLGITTLIYLIVAISMSLNGGSFFGFNGYMSSLFKNEKAGRIVFGLINICISIGVLGIINGFSM", "aa_hexdigest": "0a0cda4308aec5277add6979894ad13c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13640.043899999986, "isoelectric_point": 9.13157596588135 }, "id": "OAIKOHHJFB_892", "locus": "GCA000209735_00873" }, { "type": "cds", "sequence": "contig_1", "start": 489587, "stop": 489712, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCTATATCTATTTACCTCTTACATACTTATTTATGCCTATGTATGTAATCATGTCACTTCAAGATGGTGTTGGGGCATTAATGAATAAAGATGCTTTAACATTCGGAACAAATGCTGACTGA", "aa": "MTYIYLPLTYLFMPMYVIMSLQDGVGALMNKDALTFGTNAD", "aa_hexdigest": "8845ad847763da78011822c1c28973e5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4624.438300000001, "isoelectric_point": 4.16927661895752 }, "id": "OAIKOHHJFB_893", "locus": "GCA000209735_00874" }, { "type": "cds", "sequence": "contig_1", "start": 489851, "stop": 490003, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGAAAAACAAACAAAAGAAAAGAAGATTTCCTTTATAGCTGCTATGTTGATCGTTATAGGTGGATCAATTGGTGCTGGTATCTTCTTTAAATCAGGTGCTGTTTTAAAAAGCTCGCACAGTAGTTTGATTCTAGCCATTTTCTGTTGA", "aa": "MEEKQTKEKKISFIAAMLIVIGGSIGAGIFFKSGAVLKSSHSSLILAIFC", "aa_hexdigest": "9af944d4ff5ad9f2f66088e98547419b", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5301.333899999998, "isoelectric_point": 9.430902671813964 }, "id": "OAIKOHHJFB_894", "locus": "GCA000209735_00875" }, { "type": "cds", "sequence": "contig_1", "start": 490057, "stop": 490989, "strand": "-", "frame": 2, "gene": null, "product": "Carbamate kinase", "db_xrefs": [ "RefSeq:WP_013354651.1", "SO:0001217", "UniParc:UPI0001E33100", "UniRef:UniRef100_C4XEY2", "UniRef:UniRef50_P78030", "UniRef:UniRef90_C4XEY2" ], "nt": "ATGTCAAAAATTGTTATAGCTTTAGGTGGAAACGCTTTAGGAAACAATCCTGAAGAACAAAAAGAATTAGTTAAATTACCTGCTAAAAAAATAGCAGATTTAGTTAAAGAAGGTCACCAAGTGCTTGTTGGTCATGGTAATGGTCCTCAAGTTGGAATGATCTTCAATGCCTTTGCTGATGCAAAAAAAGTTAATGAAAAAACTCCATTAGTACCTTTTGCAGAAGCAGGTGGTATGTCTCAAGGCTACATTGGTTATCACATGCTCACAGCGATAACCAATGAATTGAGAAAGAAAAAAATTGAAAAAGATGTTTTATACTTTTTGACTCAAACAATAGTTGATGCTAAAGATAAAGCATTTAAAAACCCTACAAAGCCAGTTGGTCCTTTCTATAAAACTTTAGAAGAAGCTGAATTGGCTAATCCTAATTCAACCATTAAAGAAGATGCTGGTCGTGGTTTTAGAAAAGTTGTTCCTTCACCACTTCCTCTTGACTTTATTGGAATGAAAACAATTAGAAAAACATTTGACGCTGATAATGTTGTTATAGTTGGTGGTGGTGGTGGCATACCTACTATCGACGAAAAAGGACAATATAAAGGTGTTGATGGCGTTATCGACAAAGATTTCGCTTTAAGTAAATTGGCTACAAAAGTTGAAGCAAACTTATTTGTGATTCTTACAGCAGTAGAACATGTTTGTGTTAACTTTGGTAAACCAAACCAAAAAGCTTTAAAAGAAGTAACTGTTTCTGAACTTGAACAATACATCAAAGAAGAACAATTTGCTCCAGGAAGTATGCTTCCTAAAGTTAAAGCTGCTATTGCTTTTGTTAACGAAAATCCAAAAAATGTAGCTGTTATTGCTGACTTAAACAAAGTATCACAAGCTATCGAAGGCAAAAGTGGTACAAGAATTATCAAAGGATAA", "aa": "MSKIVIALGGNALGNNPEEQKELVKLPAKKIADLVKEGHQVLVGHGNGPQVGMIFNAFADAKKVNEKTPLVPFAEAGGMSQGYIGYHMLTAITNELRKKKIEKDVLYFLTQTIVDAKDKAFKNPTKPVGPFYKTLEEAELANPNSTIKEDAGRGFRKVVPSPLPLDFIGMKTIRKTFDADNVVIVGGGGGIPTIDEKGQYKGVDGVIDKDFALSKLATKVEANLFVILTAVEHVCVNFGKPNQKALKEVTVSELEQYIKEEQFAPGSMLPKVKAAIAFVNENPKNVAVIADLNKVSQAIEGKSGTRIIKG", "aa_hexdigest": "9389ce12ac167fa28f76f2a1b13e5d56", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E33100", "ncbi_nrp_id": "WP_013354651.1", "uniref100_id": "UniRef100_C4XEY2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33100", "RefSeq:WP_013354651.1", "UniRef:UniRef100_C4XEY2" ] }, "ips": { "uniref100_id": "UniRef100_C4XEY2", "uniref90_id": "UniRef90_C4XEY2" }, "psc": { "uniref90_id": "UniRef90_C4XEY2", "product": "Carbamate kinase", "uniref50_id": "UniRef50_P78030" }, "pscc": { "uniref50_id": "UniRef50_P78030", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78030" ], "product": "Carbamate kinase-like protein" }, "genes": [], "id": "OAIKOHHJFB_895", "locus": "GCA000209735_00876" }, { "type": "cds", "sequence": "contig_1", "start": 491046, "stop": 491294, "strand": "-", "frame": 3, "gene": null, "product": "Ornithine carbamoyltransferase chain F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4U9HLN2" ], "nt": "ATGAAAATGATTAAAGCTGCAAAAGAAGACGTTATCTTCTTACACTGCTTACCAGCTTTCCACGATGACCACACATTATTCTCAGCTGAAATCAAAGAAAAATTTGGTAAAAAATACCCAGTAGTTGCAACTGGAGCTATGGAAGTTACTGATGAAGTATTCCAATCAAAATGCAACAGATCAATTGATCAAGCTGAAAACCGTTTACACACAATTAAAGCTGTTGTTTTAGCAACTATTGGTTACTAA", "aa": "MKMIKAAKEDVIFLHCLPAFHDDHTLFSAEIKEKFGKKYPVVATGAMEVTDEVFQSKCNRSIDQAENRLHTIKAVVLATIGY", "aa_hexdigest": "3b36ec5ac8edc23a46c8307d9ab284c0", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_A0A4U9HLN2", "query_cov": 0.9634146341463414, "subject_cov": 0.9080459770114943, "identity": 0.519, "score": 80.5, "evalue": 3.27e-18, "valid": false, "product": "Ornithine carbamoyltransferase chain F", "uniref50_id": "UniRef50_A0A4U9HLN2" }, "pscc": { "uniref50_id": "UniRef50_A0A4U9HLN2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4U9HLN2" ], "product": "Ornithine carbamoyltransferase chain F" }, "genes": [], "id": "OAIKOHHJFB_896", "locus": "GCA000209735_00877" }, { "type": "cds", "sequence": "contig_1", "start": 491364, "stop": 491669, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00185.30" ], "nt": "ATGTTGGCAGACTATATGACAATTAAGGAATTCAAAGGTGATCTTAAAGGCAAAAAAATTGTCTTTGCTGGAGACATCAAAAATAATGTTGCTCGTTCAATAATGATTGGTGCTGCTTTTACAGGAATGAACTGTGTTCTATGCGGACCTAAAGAACAAGCAAAAATTGTAAAAACTGGAAAAGGTTACAAAGAAGTTTACGAAAAATGTCTAGAATTATTCAAACGTAATGGTGGATCAATTACATTTGAAGACGACAAAATCAAAGCTGCTAAAGATGCAGATGCAATCTATACAGATGTTTGA", "aa": "MLADYMTIKEFKGDLKGKKIVFAGDIKNNVARSIMIGAAFTGMNCVLCGPKEQAKIVKTGKGYKEVYEKCLELFKRNGGSITFEDDKIKAAKDADAIYTDV", "aa_hexdigest": "83345eb2642fcf610372ace96df904cb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00185.30", "name": "OTCace", "length": 86, "aa_cov": 0.8514851485148515, "hmm_cov": 0.9186046511627907, "evalue": 1.4114720238962565e-18, "score": 63.5078125, "start": 16, "stop": 101 } ], "seq_stats": { "molecular_weight": 11120.914499999988, "isoelectric_point": 8.942103385925293 }, "id": "OAIKOHHJFB_897", "locus": "GCA000209735_00878" }, { "type": "cds", "sequence": "contig_1", "start": 491703, "stop": 492089, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02729.26" ], "nt": "ATGCCATTAAACTTAAAAGGTAGGAGTTTAGACTCAGCTCTAAATTTCACTACAGATCAAATTAATGCACTTATTGACCTTGCTATTGATTTAAAAAGATCAAAAAGACAAGGGTTACATGCTAACAATCGTCCACTTGTTGGAAAAAACATTGCTATCATGTTCCAAAAAGACTCTACTAGAACACGTTGCGCCTTTGAAGTGGCAGCAGCTGACTTAGGAGCATCTTGCACATATATCGGACCTGCAGGTTCTAACTTTGGTAAAAAAGAATCTATCGAAGACACTGCACAAGTTTTAGGAAGATTTTATGATGGTATCGAATTCAGAGGATTCAAACAATCTGATGTTGATGCGTTAGTTAAATATTCTGGAGTTCCAGTTTGA", "aa": "MPLNLKGRSLDSALNFTTDQINALIDLAIDLKRSKRQGLHANNRPLVGKNIAIMFQKDSTRTRCAFEVAAADLGASCTYIGPAGSNFGKKESIEDTAQVLGRFYDGIEFRGFKQSDVDALVKYSGVPV", "aa_hexdigest": "7de699fd2dd1b14b4ad631581d29788d", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0024BB40E1", "query_cov": 1.0, "subject_cov": 0.8152866242038217, "identity": 0.586, "score": 144.0, "evalue": 6.2e-42, "valid": false, "uniref50_id": "UniRef50_A0A3D1ABF9" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02729.26", "name": "OTCace_N", "length": 126, "aa_cov": 0.9375, "hmm_cov": 0.9841269841269841, "evalue": 5.187278088160682e-31, "score": 103.83216094970703, "start": 9, "stop": 128 } ], "seq_stats": { "molecular_weight": 13940.724999999984, "isoelectric_point": 8.974337577819824 }, "id": "OAIKOHHJFB_898", "locus": "GCA000209735_00879" }, { "type": "cds", "sequence": "contig_1", "start": 492180, "stop": 492443, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACCTATCTTAATTCCTATCGCAGGTAAAGGAGCAAGTCAATTAGATATTGATATCGAAACTCACTTCGATGGTACAAACTACTTGACAATTGCACCTAGTGTTGTAGTTGGATATTCAAGAAATGAAAAAACTGAAAAAGCTCTTAAAGCAGCTAAAGTTAAAGTTTTATCATTCGAAGGAAACCAACTTTCATTAGGCATGGGTTCAGCAAGATGTATGTCAATGCCTCTTATTAGAGAAGACATTAAGAAAAAATAA", "aa": "MKPILIPIAGKGASQLDIDIETHFDGTNYLTIAPSVVVGYSRNEKTEKALKAAKVKVLSFEGNQLSLGMGSARCMSMPLIREDIKKK", "aa_hexdigest": "2f2c5d3fcf96a4992e30421335741837", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9464.039899999998, "isoelectric_point": 9.373719215393066 }, "id": "OAIKOHHJFB_899", "locus": "GCA000209735_00880" }, { "type": "cds", "sequence": "contig_1", "start": 492591, "stop": 492689, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTCGTAAAATAGCAGCGGACAAAGAAGTTAAATTCAAAAGAATTTATGCAATCAATGTTCCTCCTATGCCAAACTTAATGCACTTAGATACATGA", "aa": "MARKIAADKEVKFKRIYAINVPPMPNLMHLDT", "aa_hexdigest": "c57cbf45ae18dee8a6fdae57809795db", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3712.4553000000014, "isoelectric_point": 9.819775962829588 }, "id": "OAIKOHHJFB_900", "locus": "GCA000209735_00881" }, { "type": "cds", "sequence": "contig_1", "start": 492801, "stop": 493016, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTGGTATCGACAAAAAAGAACTTGGTATCGAATTAGATAGAGATCTTGTAGTTGACCCTATGCCAAACTTATACTTTACACGTGATCCATTTGCATCAGTTGGTAATGGTATTTCATTACACCACATGAAATATGTCACAAGACAAAGAGAAACAATATTCTCAGAATTTATCTTTGACAATAACCTTGATTACAATACAGTACCTCGTTGA", "aa": "MAGIDKKELGIELDRDLVVDPMPNLYFTRDPFASVGNGISLHHMKYVTRQRETIFSEFIFDNNLDYNTVPR", "aa_hexdigest": "57e3b907be017ce5528ff33462303180", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8262.283799999994, "isoelectric_point": 5.127866172790527 }, "id": "OAIKOHHJFB_901", "locus": "GCA000209735_00882" }, { "type": "cds", "sequence": "contig_1", "start": 493122, "stop": 493394, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATTAATGTTTACAGTGAATACGGAAAACTTAAGGAAGTACTTGTTCACACACCTGGAGACGAAATTAGGCGCATCGCGCCTTCTCGCCTCGATGAACTATTATTTTCAGCAATTTTGGAACCAGATTCTGCAATTGCGGAACATAAAAGATTTGTTCAATTATTAAAAGATAACGGAATTAAAGTTATTCAATTGGACGAATTATTCGCTAAAACTTTTGACTTGGTTAGCGAATCAGTTAAGCAATCATTAATTGAAAGATGA", "aa": "MKKINVYSEYGKLKEVLVHTPGDEIRRIAPSRLDELLFSAILEPDSAIAEHKRFVQLLKDNGIKVIQLDELFAKTFDLVSESVKQSLIER", "aa_hexdigest": "5d92807252daa81b9ba8f499ed4ff6ce", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10324.860799999991, "isoelectric_point": 6.101233863830565 }, "id": "OAIKOHHJFB_902", "locus": "GCA000209735_00883" }, { "type": "cds", "sequence": "contig_1", "start": 493690, "stop": 493836, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATGATTATGACATATGATTTTATTATGGAAATAGTAGCTAGTGTATTTCCTAAAATTTACGAAAACGAGTATAAATTAAAGGAAAACCAATATTATGATTTTGATCCTAATAGCTTAGAATTAGTAATCAAAAACAACTAA", "aa": "MAMIMTYDFIMEIVASVFPKIYENEYKLKENQYYDFDPNSLELVIKNN", "aa_hexdigest": "ed2ff75802bea58ddd481f1811b27e41", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5816.648800000003, "isoelectric_point": 4.333427619934083 }, "id": "OAIKOHHJFB_903", "locus": "GCA000209735_00884" }, { "type": "cds", "sequence": "contig_1", "start": 494044, "stop": 494625, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF11074.14" ], "nt": "TTGAATCCTTATATTCAAATCACTCACCAAGTTTCAATTATTGAAACAATTAATGGACAAATAACAAATAAAAATAATATTGTTAAAGACCCTAAAAATATCACGATTTATGATCTTCTAGACAATATTATTGCTGTTTATTCAAATAAAGCTGACTACTACATTGTTTATAACAAAGGTTATGAAAATACCAGAAACAATGAACTTATAGAATTAGCTAAACTTTATTATGCTGATAGTCAACATAAAAAAGACATGGAACACTATTTATCTATAAATAAGCTCGATATGGAAAAATTAGAACAATTAATTACTTATGTTAATGAACATACTATTGACTTATTAGATTGTTTTAAACCAATATCTAAGAATAAATTCGATGAACGTTATGGCATGGATGCTAATAAAAATTATTATGTTTTTAAAACTAATTATGTCCCTCAAGCTAAATTTCATAGTGAAAATTTAAATAAAGAAATTTTATATACTTCAGTTTCTAAAAATAGTATTCCTTTTAACGTTGAAAATTTAGCAACTTACTATAAAGACGAAAGATTTTCAACTTTCATTTATGAATTATGA", "aa": "MNPYIQITHQVSIIETINGQITNKNNIVKDPKNITIYDLLDNIIAVYSNKADYYIVYNKGYENTRNNELIELAKLYYADSQHKKDMEHYLSINKLDMEKLEQLITYVNEHTIDLLDCFKPISKNKFDERYGMDANKNYYVFKTNYVPQAKFHSENLNKEILYTSVSKNSIPFNVENLATYYKDERFSTFIYEL", "aa_hexdigest": "5dc021c36ce31ad97f9460d4e05b8c47", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF11074.14", "name": "DUF2779", "length": 125, "aa_cov": 0.6217616580310881, "hmm_cov": 0.768, "evalue": 1.2717793742475624e-13, "score": 47.455665588378906, "start": 1, "stop": 120 } ], "seq_stats": { "molecular_weight": 22937.642800000016, "isoelectric_point": 5.750025749206543 }, "id": "OAIKOHHJFB_904", "locus": "GCA000209735_00885" }, { "type": "cds", "sequence": "contig_1", "start": 494770, "stop": 495390, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTCTAAAACAACTAATAAAGATTATTTAGAAGCTAATTACTTATATCAAGTAGCTAAAAAAGCCAAAGTTGAAGTTAAAAAAATTCAAAATATTATTTTTGATGACTATACTCAAAATGGTTTTGTTTTTAGAAAAGATAAACTAAACTTAAGATTAATTGAAGCTTCAAGTTGTGGTAAAAACAAAATGGACAAAAAGGCTTTAAGAAGTGATCTTGGTTACACAATTAATGATGGTTCTTATTTTGCTTCAAAAAATGAAGCTAATGTTCATGGAGATTACCTTTTTGCCGCACTTCAAGAAGATATTTTCACTCAACCAAGTGCACGCAAAATGCCAAAAACTTCTTTAGAATTTATGAGCAAAGATCCAAGAAGTAAATTTGGAGACTTTAAAGAAGTAATAAGTAAAATTGTAGAAGCTTATTATTTAACTAAACCAACATTTTCATTTGATAATCAAATTATGTTGCCGGATTCAGATATGTTTTATGGTAAAAACAAAGATTTAATGAATAAAGTTAGAGTAGCTGTTACACCTCTTGAATTACAATATTCTCCTGCTGTCAAAAAATGTTTAAAAACTTTTGATCAAGAATACTTAAATCAATTTTGA", "aa": "MNSKTTNKDYLEANYLYQVAKKAKVEVKKIQNIIFDDYTQNGFVFRKDKLNLRLIEASSCGKNKMDKKALRSDLGYTINDGSYFASKNEANVHGDYLFAALQEDIFTQPSARKMPKTSLEFMSKDPRSKFGDFKEVISKIVEAYYLTKPTFSFDNQIMLPDSDMFYGKNKDLMNKVRVAVTPLELQYSPAVKKCLKTFDQEYLNQF", "aa_hexdigest": "32560bdf123e582d890723fb91325331", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23845.065899999998, "isoelectric_point": 9.1501428604126 }, "id": "OAIKOHHJFB_905", "locus": "GCA000209735_00886" }, { "type": "cds", "sequence": "contig_1", "start": 496082, "stop": 496225, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGCAGCTGCATTCTTAAAAGAAGAAGACTACGCCGATCCTGAAAACTTTCCAATTAATGAAAGATATTTTGCAACTTATCCTTTTAAAGTTGATAAAAAAATGGCTGAAGATGATGCAAATACCATAATTGAATTTTGA", "aa": "MIAAAFLKEEDYADPENFPINERYFATYPFKVDKKMAEDDANTIIEF", "aa_hexdigest": "59a9b8a7fb826e66fc6d94f67f779798", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5523.117300000002, "isoelectric_point": 4.214918327331543 }, "id": "OAIKOHHJFB_906", "locus": "GCA000209735_00887" }, { "type": "cds", "sequence": "contig_1", "start": 496307, "stop": 496831, "strand": "-", "frame": 1, "gene": null, "product": "MAGa7180 family putative nuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF8613E", "UniRef:UniRef90_UPI000FF8613E" ], "nt": "ATGAACAGAGTTAAAAAATTAACAAAAAGAAAATTCTATAATGGACAAGATTACATTTTAGATGAAAGAAAACAAACAATTAAATTAACAACCAATTTTCATAACACACTTTTAAATAGTTTAGGAACTTTTAATGGTTTCAAAAAATTTGGTGGTAGCTCAATTGGTAACGTCTTAGAAACAGATAGCTTCAAAAGTCATTTCCAAGCTTTTTGTTTTATGTCAAGATTAAGTATGCCAGTGTTACAAACTAAATATATAGATGCCGGGGTAGCTATAGAACCTAAAGTTTTTGAACTTATGCAAAATAAATTTAATGAAATGCATCAAAAAGATATTAAAAGAGGTAAAGTTAGTGCTGATAGTCAACCAACAAAAGTAATTCATATTGAAGCAGCAAATGTCGGATATGACTATTTTAAAGGCAAACACGACATTTTAGGTGGTGTTCCTGATGGTCTTATTCCTTCAAAAAATATTGTAGTTGAAGTTAAAACTGTTCAAGAAAAGAAAAAGGTTGATTGA", "aa": "MNRVKKLTKRKFYNGQDYILDERKQTIKLTTNFHNTLLNSLGTFNGFKKFGGSSIGNVLETDSFKSHFQAFCFMSRLSMPVLQTKYIDAGVAIEPKVFELMQNKFNEMHQKDIKRGKVSADSQPTKVIHIEAANVGYDYFKGKHDILGGVPDGLIPSKNIVVEVKTVQEKKKVD", "aa_hexdigest": "5ee56e127bb1e91fcf8a2a23d9b46c64", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI000FF8613E", "query_cov": 1.0, "subject_cov": 0.8877551020408163, "identity": 0.971, "score": 336.0, "evalue": 3.06e-116, "valid": true, "product": "MAGa7180 family putative nuclease", "uniref50_id": "UniRef50_UPI000FF8613E" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF8613E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF8613E" ], "product": "MAGa7180 family putative nuclease" }, "genes": [], "id": "OAIKOHHJFB_907", "locus": "GCA000209735_00888" }, { "type": "cds", "sequence": "contig_1", "start": 497021, "stop": 497797, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEY7", "UniRef:UniRef90_C4XEY7" ], "nt": "ATGCCTTATAAAGCTATTATTCAAAAAATATCTGAATGTGAAGAAAATAAAACAAATGTAACAAGTGTTAAAGAATTAGAAAAAATTAATGTTGAAGATGTCATTAGAATCGACGATGAATTTTTAGATCAAAAAATATTCAACAATAAAAACTTCTTATTTTTCGATTTTGAATCTAGATTAACAAAATTAAATGATAAAGTGGCAATTAGTTACTATCCAGTTCAATATTCTTCTTTTATATCAAACTTAAACAATAAAGAATTTAATGAAAAAGAAAGAAAAAACTTTTACAAAGGTGTAGTTAAAGACAACCGTAAGTTTTTCCAAATTGATGGTTTCATTTTAGATCAATATAGCAATATTTATCAAGATCAAAGTCAAAATTTAATAACAAGAGAAGAAGTTCAAAATATTTTAGATGCTAACAAAATCTTAATTTCTTATGGCATTAAAGCCGATTTAAACAACTTGAAAAAAATTGTAGGTAAAAAATATGTTGAAAGTCGATTAGTCAATTATATTGATGTTTGCAATTTTACAATGATAATTAAAAAATTAATTAGTAAAGAAAAAGAAACAATGTATAGACAAACATTATTTAATGTGGCAAAATATTTTTGTACTTATGATGATGCTTTACTTAGTGAAGAATTAAAAGAAGAATTAAAAAATAAAGATAAAAAAATAAATATACTTTTTCAAGAAGAATATAAATTTGAAAAATTGAATATGCATGATTCATTTGAAGATTCAAGAATCTTAACAATTCTTTGA", "aa": "MPYKAIIQKISECEENKTNVTSVKELEKINVEDVIRIDDEFLDQKIFNNKNFLFFDFESRLTKLNDKVAISYYPVQYSSFISNLNNKEFNEKERKNFYKGVVKDNRKFFQIDGFILDQYSNIYQDQSQNLITREEVQNILDANKILISYGIKADLNNLKKIVGKKYVESRLVNYIDVCNFTMIIKKLISKEKETMYRQTLFNVAKYFCTYDDALLSEELKEELKNKDKKINILFQEEYKFEKLNMHDSFEDSRILTIL", "aa_hexdigest": "b03ec4737b699f792aeab9e6971cef24", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 496844, "stop": 498097, "observations": { "mutation": [ 496885, 497023, 497806 ], "stop": [ 496885, 497023, 497806 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEY7", "query_cov": 1.0, "subject_cov": 0.5720620842572062, "identity": 1.0, "score": 494.0, "evalue": 1.35e-173, "gene_start": 1, "gene_end": 258, "reference_start": 135, "reference_end": 392, "reference_sequence": "MSNINGTIKKTFIEVIEELENNPSIFSKKQCYETQGYIFNKSLTADKDNNQFISLIVGDEIDTNDFFELKFFYYPKDKNKTDKEKSYSVIKKIFGFKVQTKTKQEKQNEANKRQNDLVKYSFVYTVSKNKNWHKMPYKAIIQKISECEENKTNVTSVKELEKINVEDVIRIDDEFLDQKIFNNKNFLFFDFESRLTKLNDKVAISYYPVQYSSFISNLNNKEFNEKERKNFYKGVVKDNRKFFQIDGFILDQYSNIYQDQSQNLITREEVQNILDANKILISYGIKADLNNLKKIVGKKYVESRLVNYIDVCNFTMIIKKLISKEKETMYRQTLFNVAKYFCTYDDALLSEELKEELKNKDKKINILFQEEYKFEKLNMHDSFEDSRILTILWNNVVHRFLQRELCNNLFYKKSIDDNDSKVEKRIETFRDLIFAIKYWLDKIYKEENSTN" }, "paralog": false, "identity": 0.9928057553956835, "score": 821.0, "evalue": 7.18e-305, "description": "Internal stop codon at 496885, 497023, 497806. Nonsense mutation around 496885, 497023, 497806.", "psc": { "uniref90_id": "UniRef90_C4XEY7", "uniref50_id": "UniRef50_C4XEY7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEY7", "UniRef:UniRef50_C4XEY7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEY7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEY7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 30831.941400000054, "isoelectric_point": 6.098789787292478 }, "id": "OAIKOHHJFB_908", "locus": "GCA000209735_00889" }, { "type": "cds", "sequence": "contig_1", "start": 497804, "stop": 498199, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCAATATAAACGGAACAATTAAAAAGACTTTTATCGAAGTTATTGAAGAATTAGAAAATAACCCAAGTATTTTTTCTAAAAAACAATGTTATGAAACACAAGGCTACATATTTAATAAAAGCTTAACGGCTGATAAAGATAATAATCAATTTATTTCTTTAATAGTTGGAGATGAAATTGATACTAATGATTTCTTTGAACTTAAATTCTTTTATTATCCAAAAGATAAAAATAAAACTGATAAAGAAAAATCATATTCTGTAATTAAAAAAATATTTGGTTTCAAAGTACAAACTAAAACTAAACAAGAAAAACAAAATGAAGCAAACAAAAGACAAAACGATTTAGTTAAATATAGTTTTGTTTATACAGTTTCAAAAAATAAAAATTGA", "aa": "MSNINGTIKKTFIEVIEELENNPSIFSKKQCYETQGYIFNKSLTADKDNNQFISLIVGDEIDTNDFFELKFFYYPKDKNKTDKEKSYSVIKKIFGFKVQTKTKQEKQNEANKRQNDLVKYSFVYTVSKNKN", "aa_hexdigest": "993e24a5051a319a611a075aabe5fd88", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15458.295499999987, "isoelectric_point": 9.128030204772951 }, "id": "OAIKOHHJFB_909", "locus": "GCA000209735_00890" }, { "type": "cds", "sequence": "contig_1", "start": 498315, "stop": 499070, "strand": "-", "frame": 3, "gene": null, "product": "Phosphoenolpyruvate-protein phosphotransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W6A961", "UniRef:UniRef90_A0A3P8LBF2" ], "nt": "ATGAAAGGTAAAAGAGTTGTTATTAGAACACTAGATATTGGTGGTGATAAAACTCTTAAATACTTTACATTCCCTCACGAAATGAACCCATTCTTAGGTTATCGTGCAATAAGACTTTGTCTTAAAGAAATTGAAATCTTTAAAACTCAACTTAGAGCTTTAGTTAGAGCAAGTGCTTATGGTAAAGTTGCTATTATGTTCCCAATGATAACAAACATTCCAGAATTCTTGCAAGCTAAAAAACTTTATGAAGAAGCTTATAGTGAAGTTAAAAAAGCAGGACACAAAATTGCTCCTAAAAAGGATATTGAAGTAGGTCTTATGATGGAAACCCCTGCTGCTGCTGTATTAAGCGATCAATTCTGTAAATATGCAGACTTTGTTTCGATTGGAACTAATGACTTAATTCAATACTCAATGGCTGCAGATAGAATGAGTGAAACTATTTCATACCTATATCAACCGCTTAACCCTTCTATTTTAAGATTAGTAAAAATGGTTATTGATGGTGCTCACAAACATGGTAAATGGGCTGGTATGTGCGGTGAAATGGCCGGAGATAGACGCGCATTGCCTTTACTTTTAGGTTTAGGTTTAGATGAATTTAGTATGTCTGCTGGTAATGTTTTAGCTTCAAGAGAATTGGTATCTCAATTAAGCAAAAAAGACATGGAAGAATTGGCTGCTCAAGCTATTGAATTAGATAGTGAAGAACAAGTTATTAAACTTTTAAATAAAGCTTTAAAATTAAAATAA", "aa": "MKGKRVVIRTLDIGGDKTLKYFTFPHEMNPFLGYRAIRLCLKEIEIFKTQLRALVRASAYGKVAIMFPMITNIPEFLQAKKLYEEAYSEVKKAGHKIAPKKDIEVGLMMETPAAAVLSDQFCKYADFVSIGTNDLIQYSMAADRMSETISYLYQPLNPSILRLVKMVIDGAHKHGKWAGMCGEMAGDRRALPLLLGLGLDEFSMSAGNVLASRELVSQLSKKDMEELAAQAIELDSEEQVIKLLNKALKLK", "aa_hexdigest": "421008abf5a5322aa455ec99bbb5cc7b", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI002FF43100", "query_cov": 0.9920318725099602, "subject_cov": 0.8861209964412812, "identity": 0.759, "score": 377.0, "evalue": 9.49e-130, "valid": false, "product": "phosphoenolpyruvate--protein phosphotransferase", "uniref50_id": "UniRef50_A0A938YW30" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 498315, "stop": 499370, "observations": { "mutation": [ 499121 ], "stop": [ 499121 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8LBF2", "query_cov": 1.0, "subject_cov": 0.42327150084317033, "identity": 1.0, "score": 483.0, "evalue": 3.01e-167, "gene_start": 1, "gene_end": 251, "reference_start": 343, "reference_end": 593, "reference_sequence": "MKSHKRLYVYIIKLHTYLNKMEVKMTIKGIGASRGIAVAKVFKIEELPVNITEKAKDADHELKLYNDAVKKAGEKVEATKKLAKTPEQAAIFDAHLQIINDPWALETITGRIKDNKETAEYATKAFYEEMATMFSNMDDAYMKERAADVKDVMKKLLYILNDIEEPDLTAIDKEVIIVAYDLSPSQTVQLNKKYVKGFATNIGGPTSHTAIMARSMNIPSVVGTNNVLESVKNGQTIIIDGIKGEVIVDPTKEESEHYKKEEAKYIKYLETIAKYKGKKSITKDKHHVELAANIGRPKDVDAVIDNDAEAIGLFRSEFLYMDNEHWPTEEEQFAAYKEVVERMKGKRVVIRTLDIGGDKTLKYFTFPHEMNPFLGYRAIRLCLKEIEIFKTQLRALVRASAYGKVAIMFPMITNIPEFLQAKKLYEEAYSEVKKAGHKIAPKKDIEVGLMMETPAAAVLSDQFCKYADFVSIGTNDLIQYSMAADRMSETISYLYQPLNPSILRLVKMVIDGAHKHGKWAGMCGEMAGDRRALPLLLGLGLDEFSMSAGNVLASRELVSQLSKKDMEELAAQAIELDSEEQVIKLLNKALKLK" }, "paralog": false, "identity": 0.9971509971509972, "score": 687.0, "evalue": 1.3e-249, "description": "Internal stop codon at 499121. Nonsense mutation around 499121.", "psc": { "uniref90_id": "UniRef90_A0A3P8LBF2", "product": "Phosphoenolpyruvate-protein phosphotransferase", "uniref50_id": "UniRef50_W6A961", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8LBF2", "UniRef:UniRef50_W6A961" ] }, "pscc": { "uniref50_id": "UniRef50_W6A961", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W6A961" ], "product": "Phosphoenolpyruvate-protein phosphotransferase" } }, "id": "OAIKOHHJFB_910", "locus": "GCA000209735_00891" }, { "type": "cds", "sequence": "contig_1", "start": 499119, "stop": 499709, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00391.29", "PFAM:PF02896.24" ], "nt": "ATGGCAACAATGTTTTCAAATATGGACGATGCCTACATGAAAGAAAGAGCTGCTGATGTTAAAGATGTTATGAAAAAACTTCTTTACATTTTAAATGACATTGAAGAACCTGATTTAACTGCTATTGATAAAGAAGTAATTATTGTGGCTTATGATTTAAGTCCTTCTCAAACTGTTCAATTAAATAAAAAATATGTTAAAGGTTTTGCAACCAACATTGGTGGACCAACAAGTCATACAGCAATTATGGCTAGAAGCATGAACATTCCTTCAGTAGTTGGAACAAACAATGTTTTAGAATCAGTTAAAAATGGTCAAACAATTATTATTGATGGAATCAAAGGCGAAGTAATTGTTGATCCTACAAAAGAAGAATCAGAACACTATAAAAAAGAAGAAGCTAAATACATTAAATATTTAGAAACAATTGCTAAATACAAAGGTAAAAAATCAATTACTAAAGATAAACATCATGTTGAATTAGCTGCAAATATTGGACGTCCAAAAGATGTTGATGCAGTTATTGACAATGATGCTGAAGCAATTGGGTTATTCCGTTCAGAATTCTTATACATGGATAATGAACACTGA", "aa": "MATMFSNMDDAYMKERAADVKDVMKKLLYILNDIEEPDLTAIDKEVIIVAYDLSPSQTVQLNKKYVKGFATNIGGPTSHTAIMARSMNIPSVVGTNNVLESVKNGQTIIIDGIKGEVIVDPTKEESEHYKKEEAKYIKYLETIAKYKGKKSITKDKHHVELAANIGRPKDVDAVIDNDAEAIGLFRSEFLYMDNEH", "aa_hexdigest": "61080fa331e7a2447a8542b4409ab0d0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00391.29", "name": "PEP-utilizers", "length": 73, "aa_cov": 0.37244897959183676, "hmm_cov": 0.9726027397260274, "evalue": 1.3761937534577172e-26, "score": 88.05028533935547, "start": 43, "stop": 115 }, { "id": "PF02896.24", "name": "PEP-utilizers_C", "length": 52, "aa_cov": 0.2653061224489796, "hmm_cov": 1.0, "evalue": 2.965774426149962e-13, "score": 45.6678466796875, "start": 144, "stop": 195 } ], "seq_stats": { "molecular_weight": 21956.865300000012, "isoelectric_point": 5.436558723449706 }, "id": "OAIKOHHJFB_911", "locus": "GCA000209735_00892" }, { "type": "cds", "sequence": "contig_1", "start": 499788, "stop": 500024, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAATTAAAGGAATCGGAGCAAGTCGTGGAATCGCGGTTGCAAAAGTTTTTAAAATTGAAGAACTACCAGTCAATATTACTGAAAAAGCTAAGGATGCAGATCATGAATTAAAACTATATAATGATGCTGTTAAAAAAGCTGGTGAAAAAGTTGAAGCTACTAAAAAATTAGCTAAAACTCCTGAACAAGCTGCTATTTTTGATGCGCATTTACAAATTATTAATGACCCTTGA", "aa": "MTIKGIGASRGIAVAKVFKIEELPVNITEKAKDADHELKLYNDAVKKAGEKVEATKKLAKTPEQAAIFDAHLQIINDP", "aa_hexdigest": "daf661ae6e2f783cd7f62229f659d7f7", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8483.747499999998, "isoelectric_point": 7.969017601013183 }, "id": "OAIKOHHJFB_912", "locus": "GCA000209735_00893" }, { "type": "cds", "sequence": "contig_1", "start": 500266, "stop": 500409, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTTAGCTGCTGATGTTCATGCGCCTAAGGATTTAAGAGCAAATATTCAAGCTAAAAACTTAGATGAATTTCATGTAACATTTAAAACAAAACCTAGTGATAAGATGTATTTAGCACCTGAAAAAAGAGTCAAAATCTGA", "aa": "MLLAADVHAPKDLRANIQAKNLDEFHVTFKTKPSDKMYLAPEKRVKI", "aa_hexdigest": "2456438a79a605ac355e2e761026d5e8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5393.290500000001, "isoelectric_point": 9.52199649810791 }, "id": "OAIKOHHJFB_913", "locus": "GCA000209735_00894" }, { "type": "cds", "sequence": "contig_1", "start": 500452, "stop": 500610, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGATCTTTTTGAAGGCCAAGATTCAGAATTTGGTAAATGTAATGGTAAATTAACTGTATCTGAAAATATTGCTGATGCTGGTGGAATTAGATGTGCATTAGAAGCTAGTCAAAAAGAATTAAATCATTCATCAAAAGAATTCTTTACAAACTGA", "aa": "MVDLFEGQDSEFGKCNGKLTVSENIADAGGIRCALEASQKELNHSSKEFFTN", "aa_hexdigest": "b883c3b01f53be6c7dc51cb7daaa4681", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5667.191499999998, "isoelectric_point": 4.638823509216309 }, "id": "OAIKOHHJFB_914", "locus": "GCA000209735_00895" }, { "type": "cds", "sequence": "contig_1", "start": 500656, "stop": 500865, "strand": "-", "frame": 2, "gene": null, "product": "M13-type metalloendopeptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0025523856" ], "nt": "ATGTCACCTGCTGAAGTGAATGCTTACTATAATCCTTTTATGAATCATATAGTTTTCCCTGCTGGGATTTTAGCAAAACCATTCTACTCATTAACCCAAAGTTCTTCAGCTAACTATGGTGGAATTGGAGCTGTTATTGCTCACGAAATTTCACATGCTTTTGATAATAATGGAGCTAAATTTGATGAAAAAGGTAATTTAAATTCATGA", "aa": "MSPAEVNAYYNPFMNHIVFPAGILAKPFYSLTQSSSANYGGIGAVIAHEISHAFDNNGAKFDEKGNLNS", "aa_hexdigest": "4d0f0ef07347504266be0d5db2a78593", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0025523856", "query_cov": 0.927536231884058, "subject_cov": 0.8205128205128205, "identity": 0.516, "score": 72.4, "evalue": 2.42e-15, "valid": false, "product": "M13-type metalloendopeptidase", "uniref50_id": "UniRef50_UPI0025523856" }, "pscc": { "uniref50_id": "UniRef50_UPI0025523856", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0025523856" ], "product": "M13-type metalloendopeptidase" }, "genes": [], "id": "OAIKOHHJFB_915", "locus": "GCA000209735_00896" }, { "type": "cds", "sequence": "contig_1", "start": 500869, "stop": 500988, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATAACTCCATATAAACATGAAGGTAATTTAGTAGTTAATGCATTAAATCTTAATGCAATTGAAAGCGAATATAACTTTAGTCAATACAAACAACCAATTAATAAAAATTTATGA", "aa": "MVITPYKHEGNLVVNALNLNAIESEYNFSQYKQPINKNL", "aa_hexdigest": "f06ea9320b2c4b85cf7627aa8624f18b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4508.071700000001, "isoelectric_point": 6.519455146789551 }, "id": "OAIKOHHJFB_916", "locus": "GCA000209735_00897" }, { "type": "cds", "sequence": "contig_1", "start": 501085, "stop": 501354, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATCTTAGAAACTTAATTAGATTTTCAAATCTTTTGGACAATAAAACTAGAATTACTGCAGGAGAATTTAACCGTGCAGTAAGTGGAATTGATAAAGCTTCAAGTGCAGCTAAAAGTGCTTTCTACTTAGCTTATTCATACTTTAAAATTCCTTTTGGTTTAAGATTTGCACTTGAAAATTTTGGTCCTGAAGCTAAAAGAAATGTTGAATATATGGTTCAAAAAATGATCAAAATTTATCAAGACCGTTTAAGAAACAACAGCTGA", "aa": "MYLRNLIRFSNLLDNKTRITAGEFNRAVSGIDKASSAAKSAFYLAYSYFKIPFGLRFALENFGPEAKRNVEYMVQKMIKIYQDRLRNNS", "aa_hexdigest": "a133bad68f540abfca27b32ddb24981f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10353.833199999994, "isoelectric_point": 10.05502109527588 }, "id": "OAIKOHHJFB_917", "locus": "GCA000209735_00898" }, { "type": "cds", "sequence": "contig_1", "start": 501355, "stop": 501822, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTTTTGATTTTGAAGTAATGCAAGATTTTAAAAATACCGAATTACAAACTTTAGGTTTTAGTGGACCAAGTTTAATTTTGCCAGAAAAATCATATTATGATGACAATCATCCAGCTAAACAACCGCTTTTAGAAGTATTTAAAAACATGTCAAGAAAATTATTAGCTGCTTATTTTAAAGATAAAAATAAAATTGAAGATATTATTTCTAATACATTAGAATTTGATAACTCATTAGTTAAATACACAATGAGTGCAGTTGAAAGTGCTGACTATCCCTCTTTATATAATCCTTTTACAATTGATGAAATTAATGAACATTCAAAAGTTTTTGATCTTAAAGAAATAGCAGAAAGATTAATTAAACAAGAAGTTAGCGAAAAAGTTGTTGTAATTTATCCTAAATTTATGAAAGGTTTAAATAGCTTATATAACTCAAGAACATTTGAAACATTTAAATGCTGA", "aa": "MPFDFEVMQDFKNTELQTLGFSGPSLILPEKSYYDDNHPAKQPLLEVFKNMSRKLLAAYFKDKNKIEDIISNTLEFDNSLVKYTMSAVESADYPSLYNPFTIDEINEHSKVFDLKEIAERLIKQEVSEKVVVIYPKFMKGLNSLYNSRTFETFKC", "aa_hexdigest": "9b945939e30db9d4665034f7a33c910f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18076.451199999996, "isoelectric_point": 5.122978019714354 }, "id": "OAIKOHHJFB_918", "locus": "GCA000209735_00899" }, { "type": "cds", "sequence": "contig_1", "start": 501886, "stop": 502038, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAGTGATTTAGTTAGAACTCGTGATGCAGGTGAATTAGAAGTTGGAGCAATTTTAAACTTCACAAATCTTTATTCAATGGCTAGTGATTTTACTTTTAGAAATAAATCTAAAGTTAAACCACTTAAACCTTACTTAAATAAAATTTGA", "aa": "MASDLVRTRDAGELEVGAILNFTNLYSMASDFTFRNKSKVKPLKPYLNKI", "aa_hexdigest": "0fb247e5823063ec4eb8a7e71f994ff8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5664.5136, "isoelectric_point": 9.599229621887208 }, "id": "OAIKOHHJFB_919", "locus": "GCA000209735_00900" }, { "type": "cds", "sequence": "contig_1", "start": 502246, "stop": 502674, "strand": "-", "frame": 2, "gene": null, "product": "16S rRNA (uracil(1498)-N(3))-methyltransferase", "db_xrefs": [ "EC:2.1.1.193", "KEGG:K09761", "SO:0001217", "UniRef:UniRef50_A0A449B7G8", "UniRef:UniRef90_C4XEZ0" ], "nt": "TTGCAAAAAGCTACTGAATTAGGAGTTACTACAATTATTCCTTTTGCCTCTAAATATACTGATGCAAAAATCTTAAAATATGAATTCAACAGTAAGCAAAATAGGTTTAATGAAATCATTAAAAATGCAGCTGAACAAAGCTTTAGAAACATCATTCCTTATTTAACTAAAATTATGAATTTAGAAGAAATTATTAAGACTTATCCTACTATGCAAAAGTACTTAGCTTATGAAAATCAAGACCAAAAAAATAACATTATCACTTATCCAACTAGTTCAATTTTAATAGTTGGACCTGAAGGTGGTTTTAGTCCTGAAGAAATAGAATTAGCTAAAGAAAACAATATTAAAATTACTTCATTAGGCAAAACAATCTTACGTGCTGAAACAGCTTGTTTGTATATGCTAGCTAATATCAAAGAAAAATAA", "aa": "MQKATELGVTTIIPFASKYTDAKILKYEFNSKQNRFNEIIKNAAEQSFRNIIPYLTKIMNLEEIIKTYPTMQKYLAYENQDQKNNIITYPTSSILIVGPEGGFSPEEIELAKENNIKITSLGKTILRAETACLYMLANIKEK", "aa_hexdigest": "2246d4ae69939870fd27bd08e12df65f", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 502246, "stop": 502944, "observations": { "mutation": [ 502680 ], "stop": [ 502680 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XEZ0", "query_cov": 1.0, "subject_cov": 0.6120689655172413, "identity": 0.993, "score": 280.0, "evalue": 3.54e-94, "gene_start": 1, "gene_end": 142, "reference_start": 91, "reference_end": 232, "reference_sequence": "MNERNKMHRFFVTKKSDQDHFELSKEILNHIKVARVEKDEFLCNYQDIFYKCKLENNKALIIEKLDINHEFNKPIILAAPIIKIKRFEWILQKATELGVTTIIPFASKYTDAKILKYEFNSKQNRFNEIIKNAAEQSFRNIIPYLTKIMNLEEIIKTYPTMQKYLAYENQDQKNNIITYPTSSILIVGPEGGFSPEEIELAKENNIKITSLGKTILRAETACLYMLANIKEK" }, "paralog": false, "identity": 0.9913793103448276, "score": 456.0, "evalue": 3.84e-166, "description": "Internal stop codon at 502680. Nonsense mutation around 502680.", "psc": { "uniref90_id": "UniRef90_C4XEZ0", "product": "16S rRNA (uracil(1498)-N(3))-methyltransferase", "ec_ids": [ "2.1.1.193" ], "uniref50_id": "UniRef50_A0A449B7G8", "kegg_orthology_id": "K09761", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XEZ0", "EC:2.1.1.193", "UniRef:UniRef50_A0A449B7G8", "KEGG:K09761" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449B7G8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B7G8" ], "product": "Ribosomal RNA small subunit methyltransferase E" } }, "id": "OAIKOHHJFB_920", "locus": "GCA000209735_00901" }, { "type": "cds", "sequence": "contig_1", "start": 502678, "stop": 502926, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATAGATTTTTTGTTACTAAAAAAAGTGATCAAGATCATTTTGAACTATCAAAAGAGATATTAAATCACATCAAAGTTGCACGTGTTGAAAAAGATGAATTTTTATGTAACTATCAAGACATTTTTTATAAGTGCAAATTAGAAAATAATAAAGCTTTAATTATCGAAAAATTAGATATAAATCATGAATTTAATAAGCCAATTATTCTTGCTGCACCAATTATTAAAATTAAAAGATTCGAATGA", "aa": "MHRFFVTKKSDQDHFELSKEILNHIKVARVEKDEFLCNYQDIFYKCKLENNKALIIEKLDINHEFNKPIILAAPIIKIKRFE", "aa_hexdigest": "da417bb2bf34f539d5efe3f686e7eaf7", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9904.495299999988, "isoelectric_point": 8.566639518737794 }, "id": "OAIKOHHJFB_921", "locus": "GCA000209735_00902" }, { "type": "cds", "sequence": "contig_1", "start": 503091, "stop": 504239, "strand": "-", "frame": 3, "gene": "ftsZ", "product": "cell division protein FtsZ", "db_xrefs": [ "GO:0000910", "GO:0003924", "GO:0005525", "KEGG:K03531", "RefSeq:WP_013354660.1", "SO:0001217", "UniParc:UPI0001E33107", "UniRef:UniRef100_C4XEZ1", "UniRef:UniRef50_C4XEZ1", "UniRef:UniRef90_C4XEZ1" ], "nt": "ATGGACTTAAATGCTGAAGATTTGGAAGTTAAACTAAAAGTCATTGGTGTTGGTGGTGCAGGTAATAACGCCATTAACTTGATGCTTGATGAAAACTTGCCAAATGTTGAATTACTAGTTGCTAACTCAGATCGTCAAGATTTGGTTAAAAGCTTATGTCCAAATAAAATACTTTTAGGTGATTCTACCAGAGGCTTTGGTGCTGGTGGGGACCCTAAAGTAGGACGTGAGTGCGCACTAGAAAGCATTAAAGAAATTCAAAAATCATTAGAAAACACAGATATTGTTATTATTTCAGCTGGCTTAGGCGGCGGAACAGGAACTGGAGCAGCACCAGTGATTGCCGAAGCTGCCAAAAAGATGGGCATTTTGACAGTGGCAGTAGTAACCACTCCTTTTGAATTAATTGAAGGAAAACATAAAAGCTTAATTGCTCAAGAAGGCTTGAAAAAATTAAGTGAAGTTGTTGATAGTTATATTGTAATTTCAAACCAAAAATTGGTTGAAAATTACAGAAACTTACCAGTTCAAGAAGCTTTTAAAGTTTCTAACTATACATTGAAAAATTCAATCAAAATAATTCGTGATATTATCTTTGAAACTGGGTTTATTAATTTAGATTTCAATGATTTACGTCAAGTTTTATTAGATGGAAAAGAAACAATTATAGGAATTGGTAATGGCTTTGGCAAAGACCGAGCTATTAAAGCAGTTGATGATGCTTTAATGACTCCACTTTTTCAAAGTGAAATTAAAAATTGTCAAAAAGTTGCTATTTTATTTCAGTGTGATAAAAGAGCAAGTTTAGATGACATTGAAACAGCAAAAAATCGAATTGATGAGTATTTAGCAAATAACCTTGAAGCTCAAACTTTTATCGGACTTCAATATATCGATACTCAAGATCGTGAAGAAATATTCAGAATTAGTATTATTGCTTCAAATCTTAATGCAAATGTTTCAACAAATACTTATGCGGTACCAAAATTATTGCAAAGAAATGTTAGTGAACATTATGAGCAAAATATGCAAAATACATATACACAAATTAATGGATCACATCATACTCGTATAATGAGTTATACTGCTCAAAACTATGAAGATGAAAATGAAGATGTAATGCCAAATTTCTTTGATGAAAATCATTAA", "aa": "MDLNAEDLEVKLKVIGVGGAGNNAINLMLDENLPNVELLVANSDRQDLVKSLCPNKILLGDSTRGFGAGGDPKVGRECALESIKEIQKSLENTDIVIISAGLGGGTGTGAAPVIAEAAKKMGILTVAVVTTPFELIEGKHKSLIAQEGLKKLSEVVDSYIVISNQKLVENYRNLPVQEAFKVSNYTLKNSIKIIRDIIFETGFINLDFNDLRQVLLDGKETIIGIGNGFGKDRAIKAVDDALMTPLFQSEIKNCQKVAILFQCDKRASLDDIETAKNRIDEYLANNLEAQTFIGLQYIDTQDREEIFRISIIASNLNANVSTNTYAVPKLLQRNVSEHYEQNMQNTYTQINGSHHTRIMSYTAQNYEDENEDVMPNFFDENH", "aa_hexdigest": "61bfc8f8ae42dae9f9463bea5f770824", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001E33107", "ncbi_nrp_id": "WP_013354660.1", "uniref100_id": "UniRef100_C4XEZ1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33107", "RefSeq:WP_013354660.1", "UniRef:UniRef100_C4XEZ1" ] }, "ips": { "uniref100_id": "UniRef100_C4XEZ1", "uniref90_id": "UniRef90_C4XEZ1" }, "psc": { "uniref90_id": "UniRef90_C4XEZ1", "gene": "ftsZ", "product": "cell division protein FtsZ", "uniref50_id": "UniRef50_C4XEZ1", "kegg_orthology_id": "K03531", "go_ids": [ "GO:0000910", "GO:0003924", "GO:0005525" ] }, "pscc": { "uniref50_id": "UniRef50_C4XEZ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEZ1" ], "product": "Cell division protein FtsZ" }, "genes": [ "ftsZ" ], "id": "OAIKOHHJFB_922", "locus": "GCA000209735_00903" }, { "type": "cds", "sequence": "contig_1", "start": 504324, "stop": 504620, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTTCTTTATTTGACCAAGCTATTCATAAAGTTAAAGCGTTTTTAGACCAAAATAATCAAAAAGTTGATGAGTTGGTTTTAAACTGCAAAAACTCAGAAATCTTTATGGATCGTTTTAACAAATTATATCCCGAAATGAATGTTGCAATTCTAGACGATAACGTCTCAACTTTATTCAATATACCTAATAGCCTTTTAGGCATTATTAATTTACTAAATCCTGAAAAAATCAATTGTGAAGACATGGTTAATACTTTGAATAATTTACCAGAATACAAACCAAAAAGTTTTTGA", "aa": "MISLFDQAIHKVKAFLDQNNQKVDELVLNCKNSEIFMDRFNKLYPEMNVAILDDNVSTLFNIPNSLLGIINLLNPEKINCEDMVNTLNNLPEYKPKSF", "aa_hexdigest": "bd784c4c0456baab6732eb2f6dcc214c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11317.950999999986, "isoelectric_point": 4.780182075500488 }, "id": "OAIKOHHJFB_923", "locus": "GCA000209735_00904" }, { "type": "cds", "sequence": "contig_1", "start": 504675, "stop": 504806, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCTAAAACATTTTTCTATGAAAAATCTAACATTGACTTTAAAAACTTTGTTAATTATTTATCAAAAGAATTAAATAAAAGTAAAGAAGATACAATTTTGTTACTTGAAGCTGTGGTAGCTAATTGA", "aa": "MNAKTFFYEKSNIDFKNFVNYLSKELNKSKEDTILLLEAVVAN", "aa_hexdigest": "56f21b868eee47f2cdce876dd7497f23", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5030.702700000001, "isoelectric_point": 6.089979743957519 }, "id": "OAIKOHHJFB_924", "locus": "GCA000209735_00905" }, { "type": "cds", "sequence": "contig_1", "start": 505149, "stop": 505457, "strand": "-", "frame": 3, "gene": null, "product": "MAG3720 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FEECA7D", "UniRef:UniRef90_UPI000FEECA7D" ], "nt": "ATGAAAAAAATTTATGTAAATTTCTTTCTTAAAAAAACTAGACTTGATGTGACAGTAGTGGAAGATATTAATTCTAAATATTTTCCAGTTTTTCAAACATATCTAGAAACCAAAAACTTTGAAGCGAATAAGATTAAAGAATTTATTACGAATTTCAAAAAATATTTGAAATCTAAAGTTGCTACCAAGAAAAATGATGTAGTTAAATATTCTTTTATAGTTAAAGATAGTTTATACAAAAGTTTAAAATTTGTTAACCAAACTTTGACTTTTGATGTTCCAAGTAATACAGTTGATCAACAAGTATGA", "aa": "MKKIYVNFFLKKTRLDVTVVEDINSKYFPVFQTYLETKNFEANKIKEFITNFKKYLKSKVATKKNDVVKYSFIVKDSLYKSLKFVNQTLTFDVPSNTVDQQV", "aa_hexdigest": "36e174daa2dba9331720acc57107e6a9", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 504994, "stop": 505457, "observations": { "deletion": [ 505043 ], "mutation": [ 505151 ], "stop": [ 505151 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000FEECA7D", "query_cov": 1.0, "subject_cov": 0.6623376623376623, "identity": 1.0, "score": 199.0, "evalue": 6.97e-64, "gene_start": 1, "gene_end": 102, "reference_start": 1, "reference_end": 102, "reference_sequence": "MKKIYVNFFLKKTRLDVTVVEDINSKYFPVFQTYLETKNFEANKIKEFITNFKKYLKSKVATKKNDVVKYSFIVKDSLYKSLKFVNQTLTFDVPSNTVDQQVWKEVKNKLEEQKESSTYELINSNIYLYETINNDVVKKLQYFSLKQTSSKIKY" }, "paralog": false, "identity": 0.9935064935064936, "score": 294.0, "evalue": 8.64e-104, "description": "Internal stop codon at 505151. Frameshift due to deletion around 505043. Nonsense mutation around 505151.", "psc": { "uniref90_id": "UniRef90_UPI000FEECA7D", "product": "MAG3720 family protein", "uniref50_id": "UniRef50_UPI000FEECA7D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000FEECA7D", "UniRef:UniRef50_UPI000FEECA7D" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000FEECA7D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FEECA7D" ], "product": "MAG3720 family protein" } }, "id": "OAIKOHHJFB_925", "locus": "GCA000209735_00906" }, { "type": "cds", "sequence": "contig_1", "start": 505507, "stop": 506469, "strand": "-", "frame": 2, "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "db_xrefs": [ "EC:2.1.1.199", "GO:0016434", "KEGG:K03438", "SO:0001217", "UniParc:UPI0001A8C25A", "UniRef:UniRef100_C4XEZ3", "UniRef:UniRef50_Q6KHR2", "UniRef:UniRef90_A0A3P8MEW3" ], "nt": "ATGACAGTGACATATCTTCAGCAGCACAAAAAATTCTTGAAAGAAGTGGTAAATAAAATGGAGAATCAACACTTTTCTGTTTTACTGCAAGAGTCTATTGATGCCCTTAATATAAAACCAGATGGTATTTATGTTGATTTAACTTTGGGCATGGGTGGACATTCTAGCGAGATTTTAAAGAGACTTGACACTGGTCGTTTGATTGCTTTTGATAAGGACACTTTTGCGATAGAAGAAAGTCGCAAACGTCTCAGCCAAATAAAATCAACTAATTTTCAACTAATTCATTCTGACTTCAAAAATATAAAAAGTGAATTAGATAAATTGAACATTAGCCAAGTAGATGGAATCATTGCTGATTTAGGTATTAGCAGTCCACAAGTTGATAATGATGAACGAGGTTTCTCTTATAACAAAAATGCACGTCTTGACATGCGCATGGATCAAAGTCAAAAGCTTGATGCCTACTATGTAGTTAATAATTATAGTGAAAGTCAATTGGAACAATTATTAAGAAACTACGCTGAGGTAAAACTTGCTCGTCAGGTTGCGAAGGCCATCATTAGTAAGCGGCCAATAACTACCACTTTGGAATTAGCTCAAGTAGTGCGAGATGCTTATCCTGCAAAATTAGTGAAACTAAAAAATCCTAATAAAGCAGTGTTCCAAGCTATCCGTATTGAAGTTAATAATGAATTTGATTCCCTTAAGCAAATGTTACAAGATGCTATTGAACTATTAAAACCTAATACTTCTTTAGCCGTTATAACTTTTCATTCATTGGAAGACAGCATTGTTAAACATTTTTTTGGTTCACTAATTAAAAGTAAATTACCAGCAAAAATGCCAATTAATGAAGAGAAGCAATATAAAGTAAAAGTATTTCTTCCAACCAATAATGAGTTAGAACTTAATAAACGCTCTCGTAGCGCCAAGTTAAGAGTTTTGAAAAAATGCTTTTAA", "aa": "MTVTYLQQHKKFLKEVVNKMENQHFSVLLQESIDALNIKPDGIYVDLTLGMGGHSSEILKRLDTGRLIAFDKDTFAIEESRKRLSQIKSTNFQLIHSDFKNIKSELDKLNISQVDGIIADLGISSPQVDNDERGFSYNKNARLDMRMDQSQKLDAYYVVNNYSESQLEQLLRNYAEVKLARQVAKAIISKRPITTTLELAQVVRDAYPAKLVKLKNPNKAVFQAIRIEVNNEFDSLKQMLQDAIELLKPNTSLAVITFHSLEDSIVKHFFGSLIKSKLPAKMPINEEKQYKVKVFLPTNNELELNKRSRSAKLRVLKKCF", "aa_hexdigest": "ca69d16297885b96282fb4baac62a1bb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C25A", "uniref100_id": "UniRef100_C4XEZ3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C25A", "UniRef:UniRef100_C4XEZ3" ] }, "ips": { "uniref100_id": "UniRef100_C4XEZ3", "uniref90_id": "UniRef90_A0A3P8MEW3" }, "psc": { "uniref90_id": "UniRef90_A0A3P8MEW3", "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "ec_ids": [ "2.1.1.199" ], "uniref50_id": "UniRef50_Q6KHR2", "kegg_orthology_id": "K03438", "go_ids": [ "GO:0016434" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KHR2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KHR2" ], "product": "Ribosomal RNA small subunit methyltransferase H" }, "genes": [ "rsmH" ], "id": "OAIKOHHJFB_926", "locus": "GCA000209735_00907" }, { "type": "cds", "sequence": "contig_1", "start": 506507, "stop": 506635, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGCAATACTCAAGAAATTGAACTTGACAACCAAGGACGTTTCGTTGTTCCAAAACAATTCCTCGACAAAGCTGCTATCCAAAAAGAAGCCATTTTTGTAGCCTCAGGTAATCTTGTAGAACTTTGA", "aa": "MSNTQEIELDNQGRFVVPKQFLDKAAIQKEAIFVASGNLVEL", "aa_hexdigest": "e6c6179a47da3362b311faa7d14a7294", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4692.305799999999, "isoelectric_point": 4.655875205993653 }, "id": "OAIKOHHJFB_927", "locus": "GCA000209735_00908" }, { "type": "cds", "sequence": "contig_1", "start": 506636, "stop": 506848, "strand": "-", "frame": 1, "gene": "mraZ", "product": "Transcriptional regulator MraZ", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_F9UK44", "UniRef:UniRef90_A0A3P8KXP2" ], "nt": "ATGTTTGGACAATACAAAAGAAACATTGACGACAAAAAAAGATTAGCTTTACCATCTAAATTGCGTGACGATTTAGGAAGCAAAATTTATTTAACTGTCGGACTTGAAGGCATTGTTGAAATTAGAAGCGAAAAAGAATTCAATAAATTCGTTGAAGTTCTAAATCAACAAAACTCTTTTGATACAAATGCTCGTTTTGTTAGAAGATTTTGA", "aa": "MFGQYKRNIDDKKRLALPSKLRDDLGSKIYLTVGLEGIVEIRSEKEFNKFVEVLNQQNSFDTNARFVRRF", "aa_hexdigest": "fc1bcb8076e3e37f9a1362ad29c21bbf", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "mraZ" ], "truncated": "5-prime", "pseudogene": { "start": 506414, "stop": 506878, "observations": { "mutation": [ 506509, 506638 ], "stop": [ 506509, 506638 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8KXP2", "query_cov": 1.0, "subject_cov": 0.45454545454545453, "identity": 1.0, "score": 142.0, "evalue": 6.29e-42, "gene_start": 1, "gene_end": 70, "reference_start": 11, "reference_end": 80, "reference_sequence": "MVVDSGRKWKMFGQYKRNIDDKKRLALPSKLRDDLGSKIYLTVGLEGIVEIRSEKEFNKFVEVLNQQNSFDTNARFVRRFWLSNTQEIELDNQGRFVVPKQFLDKAAIQKEAIFVASGNLVELWGSEKFDQYCQNFNDSDISSAAQKILERSGK" }, "paralog": false, "identity": 0.9805194805194806, "score": 295.0, "evalue": 6.58e-105, "description": "Internal stop codon at 506509, 506638. Nonsense mutation around 506509, 506638.", "psc": { "uniref90_id": "UniRef90_A0A3P8KXP2", "product": "Transcriptional regulator MraZ", "uniref50_id": "UniRef50_F9UK44", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8KXP2", "UniRef:UniRef50_F9UK44" ] }, "pscc": { "uniref50_id": "UniRef50_F9UK44", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_F9UK44" ], "product": "Transcriptional regulator MraZ" } }, "id": "OAIKOHHJFB_928", "locus": "GCA000209735_00909" }, { "type": "cds", "sequence": "contig_1", "start": 507092, "stop": 507769, "strand": "-", "frame": 1, "gene": null, "product": "Potassium uptake protein A", "db_xrefs": [ "RefSeq:WP_041594217.1", "SO:0001217", "UniParc:UPI0003191539", "UniRef:UniRef100_C4XEZ5", "UniRef:UniRef50_C4XEZ5", "UniRef:UniRef90_C4XEZ5" ], "nt": "ATGCCTCGTAAAAGAAAAAATGAATATATCTGTGTTATTGGAACAGGGCGTTTTGGTAGTGCGGTTGTAAGCCAACTTGCTAAAATGAATTGTCCTTTATTATTAATAGATAAAGATGAAGAAGTTTTAAAATCATATGATGATTTAGCACAAAAAGTTGTTATTGCTGATGCAGGTGATATGAAAGCTTTAAGAGCACTTAATCTTCAAGAAATTGGAACTGTTGTTGTTGCTGTTCCTGATAATATTGAAATTGTGGCTGCTTTACTCGAATTAAAAGTTAATAATATTATTGCTCGTGCAACAAGTAAAAGACATGCACGTGTATTAAAACAAATTGGAGTTAATGTTATTGTTAGACCTGAATATGAAGCCGGTGTTCGTACTGCTCTTTTAGCAGCTAATCCAAATTTTAGCCGTTATTCTCAAACATTACAAGAAATTGGCGATGGCTTCGTTATGGGTACTACTATTTTAAATAATCCAAAATTAATCAATAAACCAATCAAAGAATTAAAATTCTTTAACTTAGGAATTTCAGTTGTTTTAATTAAGAGAGGAACTGAAAGTATTTTACCTACTGGGGTAACTGTTTTACAAAAAAGTGATCTTATTACAATTCTAGGTAAGGTTGAAGATGTAACCAATGCTTTTGGTTTCTTAAATGTCGAAGACTAG", "aa": "MPRKRKNEYICVIGTGRFGSAVVSQLAKMNCPLLLIDKDEEVLKSYDDLAQKVVIADAGDMKALRALNLQEIGTVVVAVPDNIEIVAALLELKVNNIIARATSKRHARVLKQIGVNVIVRPEYEAGVRTALLAANPNFSRYSQTLQEIGDGFVMGTTILNNPKLINKPIKELKFFNLGISVVLIKRGTESILPTGVTVLQKSDLITILGKVEDVTNAFGFLNVED", "aa_hexdigest": "7a628c9151db2f41c7e4389389d832c4", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0003191539", "ncbi_nrp_id": "WP_041594217.1", "uniref100_id": "UniRef100_C4XEZ5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003191539", "RefSeq:WP_041594217.1", "UniRef:UniRef100_C4XEZ5" ] }, "ips": { "uniref100_id": "UniRef100_C4XEZ5", "uniref90_id": "UniRef90_C4XEZ5" }, "psc": { "uniref90_id": "UniRef90_C4XEZ5", "product": "Potassium uptake protein A", "uniref50_id": "UniRef50_C4XEZ5" }, "pscc": { "uniref50_id": "UniRef50_C4XEZ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEZ5" ], "product": "Potassium uptake protein A" }, "genes": [], "id": "OAIKOHHJFB_929", "locus": "GCA000209735_00910" }, { "type": "cds", "sequence": "contig_1", "start": 508225, "stop": 508434, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGGACAAGCAATAATTGCAATCCTGATCTTTTTAGGAGGATTAGGTATTTTTGCCTTAAGAATTTTCTTGATTAATTTAATCTTTTTTAAACGTAGAAATTCTTTAAGTGAGCTTGAAGTTGTGTCACATGAAAGAGGCAGCGGTGATAGTGGTCAAACTAAAAAATTAATTATGGACTCAATTGGAACCTTATTAATTATTTGA", "aa": "MFGQAIIAILIFLGGLGIFALRIFLINLIFFKRRNSLSELEVVSHERGSGDSGQTKKLIMDSIGTLLII", "aa_hexdigest": "72e2093f0a50a4cc2a10b6822ff0e2da", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7607.031999999997, "isoelectric_point": 9.687744712829591 }, "id": "OAIKOHHJFB_930", "locus": "GCA000209735_00911" }, { "type": "cds", "sequence": "contig_1", "start": 508624, "stop": 508767, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTTACTACCACAATATTGGATTACAAATTATTTTCTTGACTCTTTTAATAATTGGTGGTTTAGGTTATCCTGTAATTCATGATATTTTAAACTTCTTTAGATTCTTAATCAAATACAAAGGTAAAAGAAGATATCAATGA", "aa": "MPYYHNIGLQIIFLTLLIIGGLGYPVIHDILNFFRFLIKYKGKRRYQ", "aa_hexdigest": "b42de4efa5623b2c760ac6ac8621978d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5657.7618999999995, "isoelectric_point": 9.993905067443851 }, "id": "OAIKOHHJFB_931", "locus": "GCA000209735_00912" }, { "type": "cds", "sequence": "contig_1", "start": 509019, "stop": 509429, "strand": "+", "frame": 3, "gene": "trkG", "product": "potassium transporter TrkG", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AD00B6", "UniRef:UniRef90_UPI0021AD00B6" ], "nt": "ATGTTCATTGGTGCTGCTCCAGCTTCAACTGGTGGCGGTATTAGAACTACCACTATGGCAATATTATTCTTATCATTATTCTCAAAAATTATGGGTCGACCAAGTGTTAGAGCTTTTAGAAGAAGAATTGATGATGAAACAGTTAGAATGTCATCAATAGTTTTCTCAATTAGTATTTTCTTAATAATATTTATTTCATTTATTACCATGTCAAGTTTAAACAATTACAGTGGACAAATCGATTCAAACAAATATAATTTCATTCATATTTTCTTTGAAGTTAACTCAGCATTTGGAACTACAGGATTAACTTGTGGTATTACTGATAAATTAAATAATGCTTCAAAAGTTTTCTTAAGTATATTGATGTTCATTGGACAATTTGGTATTAGTAGCACAATATTAGTTTGA", "aa": "MFIGAAPASTGGGIRTTTMAILFLSLFSKIMGRPSVRAFRRRIDDETVRMSSIVFSISIFLIIFISFITMSSLNNYSGQIDSNKYNFIHIFFEVNSAFGTTGLTCGITDKLNNASKVFLSILMFIGQFGISSTILV", "aa_hexdigest": "83415cf343d9ab6d60be54f0641ea146", "start_type": "ATG", "rbs_motif": null, "genes": [ "trkG" ], "truncated": "3-prime", "pseudogene": { "start": 508968, "stop": 509492, "observations": { "mutation": [ 509427 ], "stop": [ 509427 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0021AD00B6", "query_cov": 1.0, "subject_cov": 0.7816091954022989, "identity": 1.0, "score": 256.0, "evalue": 7.61e-86, "gene_start": 1, "gene_end": 136, "reference_start": 18, "reference_end": 153, "reference_sequence": "MNFSKLTSTSLIICSILMFIGAAPASTGGGIRTTTMAILFLSLFSKIMGRPSVRAFRRRIDDETVRMSSIVFSISIFLIIFISFITMSSLNNYSGQIDSNKYNFIHIFFEVNSAFGTTGLTCGITDKLNNASKVFLSILMFIGQFGISSTILVWGKKKNYSNKYEYISEPVAIG" }, "paralog": false, "identity": 0.9885057471264368, "score": 332.0, "evalue": 7.57e-118, "description": "Internal stop codon at 509427. Nonsense mutation around 509427.", "psc": { "uniref90_id": "UniRef90_UPI0021AD00B6", "product": "potassium transporter TrkG", "uniref50_id": "UniRef50_UPI0021AD00B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0021AD00B6", "UniRef:UniRef50_UPI0021AD00B6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0021AD00B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AD00B6" ], "product": "potassium transporter TrkG" } }, "id": "OAIKOHHJFB_932", "locus": "GCA000209735_00913" }, { "type": "cds", "sequence": "contig_1", "start": 510028, "stop": 510777, "strand": "+", "frame": 1, "gene": "rpiR", "product": "RpiR family transcriptional regulator", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF00", "UniRef:UniRef90_C4XF00" ], "nt": "ATGACAAATGCTGAACAAAAAATTATTGATTATATCAATGAAAATCCTAATGAGTTTGCAGAAAACTCAATTGAAAAAAATGCTGTCGAGACTTTTGCAAGTACAGCCGTTTTATCTAGAATTTACAAAAAATTAGGATTTTCAAGTTATAAGGATATGCAATTTTATGTTAAGTATAATTATTTGTCAAATTTAAAAATACCGAAAGAATTACTAGATAATTCTATTTATAAAATAGCTAAAACTTATATGGAAGCTATTAATTTAACAAGCAAATATTTAGATATAAAAGAAATTGATTATATTTGTGAAGAAATAAACAAAAGCAAACTGGTTTATTGTTTTGGAATTGGTTCAAGTTCAATCAGTGCTAAAGAATTAAGCTTGAACATTGAAAAATTTGATTACTACAGTGTCTTCCATAATGATTTTCATAACTTCTTAATTTCTTTATATAGTCATGAATTAAAAGAAAATAAACCACCTGTTATTTTGTTTTCTAAAAGTGGTAAAACAAATGAGATTTTGTTTTTATGTGAAACATTATTAGAACACAAATTTCCATTTTTATTAATAACTGCAAATAGATCACTTAAAAATAAATATCCTCACGTTTTATTGCATGAAACAATTGAACAAAAAGAAAGATATCATGCTTTAAGTTCAAAAATTGTTCAACAATATATAGCTGATATTATTGCAGATCATTTAATTTCAAACAGTCCAAACAAATTAAATCGTAAGAATTGA", "aa": "MTNAEQKIIDYINENPNEFAENSIEKNAVETFASTAVLSRIYKKLGFSSYKDMQFYVKYNYLSNLKIPKELLDNSIYKIAKTYMEAINLTSKYLDIKEIDYICEEINKSKLVYCFGIGSSSISAKELSLNIEKFDYYSVFHNDFHNFLISLYSHELKENKPPVILFSKSGKTNEILFLCETLLEHKFPFLLITANRSLKNKYPHVLLHETIEQKERYHALSSKIVQQYIADIIADHLISNSPNKLNRKN", "aa_hexdigest": "d0c1eeafbd8e280d80748607b5a0287d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XF00", "query_cov": 1.0, "subject_cov": 0.9021739130434783, "identity": 0.996, "score": 484.0, "evalue": 1.65e-172, "valid": true, "product": "RpiR family transcriptional regulator", "uniref50_id": "UniRef50_C4XF00" }, "pscc": { "uniref50_id": "UniRef50_C4XF00", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF00" ], "product": "RpiR family transcriptional regulator" }, "genes": [ "rpiR" ], "id": "OAIKOHHJFB_933", "locus": "GCA000209735_00914" }, { "type": "cds", "sequence": "contig_1", "start": 510852, "stop": 511547, "strand": "-", "frame": 3, "gene": null, "product": "N-acetylmannosamine-6-phosphate 2-epimerase", "db_xrefs": [ "RefSeq:WP_013354668.1", "SO:0001217", "UniParc:UPI0001E3310F", "UniRef:UniRef100_A0AB32XC18", "UniRef:UniRef50_A0A097SSE0", "UniRef:UniRef90_A0AB32XC18" ], "nt": "ATGAGTCTTAAAAATAATTTGTTTATAGTTTCTTGTCAAGCATTGGAAAATGAGCCAATGTATGGTCCTGGTGTAATCGAAAGAATGGTTAAGTCTGTTTTATTAGGTGGTGCTCAAGGCATAAGAATTAGCCAAAGAGAAAATATTGAACCAGTCTTAAGAATAGTACCTAAAGACATTCCTGTTATTGGTATTACTAAAAAAATGTCTAAAGATTCTGAAGTATTTATTACTCCTGATTTAGATGATTTAAAATTCTTAATTGAAAGTAAATGTCCAATAATTGCAATAGATGCAACTTTAAGAAAAAGACCAAAAGAAAGTTTGGAAGACTTAGTTCAATATTTCAAGAAAAATAATCATAATCAGTTATTAATGGCAGATTGCTCTTCAATTGAAGATGTTGAAAATGCTTTTAAATTAGATTTTGATATTGTTTCAACTACTCTTAGAGGCTACACAAAAGACACATTAAATAAACCTAATAGTTACAATGATTTTGAATTTCTAAAAGAAGTAGTTGAATTAAATAAAAAGTACAAAAAATACATAATTGCTGAAGGCGGATTTAATACACCTGAAATGGTCCAAAAAGCCTATAAAATAGGTGCAAATGGTGTAGTTGTTGGCTCAGCAATTACTAGACCAACTTTTATAACAAAACAATTTGTTGACTATTTAAGCAATAAAAAATAA", "aa": "MSLKNNLFIVSCQALENEPMYGPGVIERMVKSVLLGGAQGIRISQRENIEPVLRIVPKDIPVIGITKKMSKDSEVFITPDLDDLKFLIESKCPIIAIDATLRKRPKESLEDLVQYFKKNNHNQLLMADCSSIEDVENAFKLDFDIVSTTLRGYTKDTLNKPNSYNDFEFLKEVVELNKKYKKYIIAEGGFNTPEMVQKAYKIGANGVVVGSAITRPTFITKQFVDYLSNKK", "aa_hexdigest": "982de2b82f08ad3af98b8d5627f9c901", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E3310F", "ncbi_nrp_id": "WP_013354668.1", "uniref100_id": "UniRef100_A0AB32XC18", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3310F", "RefSeq:WP_013354668.1", "UniRef:UniRef100_A0AB32XC18" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XC18", "uniref90_id": "UniRef90_A0AB32XC18" }, "psc": { "uniref90_id": "UniRef90_A0AB32XC18", "product": "N-acetylmannosamine-6-phosphate 2-epimerase", "uniref50_id": "UniRef50_A0A097SSE0" }, "pscc": { "uniref50_id": "UniRef50_A0A097SSE0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A097SSE0" ], "product": "Putative N-acetylmannosamine-6-phosphate 2-epimerase" }, "genes": [], "id": "OAIKOHHJFB_934", "locus": "GCA000209735_00915" }, { "type": "cds", "sequence": "contig_1", "start": 512074, "stop": 512277, "strand": "-", "frame": 2, "gene": null, "product": "PTS transporter subunit EIIC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00168119FF" ], "nt": "ATGGGTATTGGTTTAGCTATGATTCTTTTAGCTGAAAAAGAAAATCGTAAAAATGTAGCGATAACAATAGGTTCAGCAATGGGTGTTGCATTCTTAACAGGTATTACAGAACCTCTTGAATTTACATTCTTGTTTATTGCTCCTCAACTTTATTATGCTATTTATGTACCACTTAGTGGTTTAGCATATGCATTAATGGAATGA", "aa": "MGIGLAMILLAEKENRKNVAITIGSAMGVAFLTGITEPLEFTFLFIAPQLYYAIYVPLSGLAYALME", "aa_hexdigest": "27feea9e1a1b1384811f86f00fb7d837", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00168119FF", "query_cov": 0.9253731343283582, "subject_cov": 0.8611111111111112, "identity": 0.5, "score": 50.8, "evalue": 6.31e-07, "valid": false, "product": "PTS transporter subunit EIIC", "uniref50_id": "UniRef50_UPI00168119FF" }, "pscc": { "uniref50_id": "UniRef50_UPI00168119FF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00168119FF" ], "product": "PTS transporter subunit EIIC" }, "genes": [], "id": "OAIKOHHJFB_935", "locus": "GCA000209735_00916" }, { "type": "cds", "sequence": "contig_1", "start": 512404, "stop": 512658, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAGATCACCAATAGGTTTAGATGGATTTATTTATGGTATATTAGGCCGTGCTTTAATGCCTTTTGGTTTACACCACATTCCAATTGTTTTAGCATATCAAACTGAATTTGGTGGTGTGTTATCTAAGGAAATGATTGATGCGGCTGTAACAGCTGGTAAAATTTCTTCAAAAGTAGCTACTGATTTAAAAAATTCATTAGAGAGTTTTTCTAAAGGAAAAGATGTTATTACTGGTGACCAAAACATCTGA", "aa": "MQRSPIGLDGFIYGILGRALMPFGLHHIPIVLAYQTEFGGVLSKEMIDAAVTAGKISSKVATDLKNSLESFSKGKDVITGDQNI", "aa_hexdigest": "a473ba75fcc88d38b11e284fafc49bdf", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8979.317399999998, "isoelectric_point": 6.709013175964355 }, "id": "OAIKOHHJFB_936", "locus": "GCA000209735_00917" }, { "type": "cds", "sequence": "contig_1", "start": 512698, "stop": 512883, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATCATTAAATACAAGTATTTTTGGTGGTATGGTTATTGGAATTGTCACTGCATATACATTTAATTATTTCTCTAAAATTGAATTACCACCTGCACTTTCATTCTTCTCAGGAATTAGATTAGTGCCAATTATTTTAGTACCTATTTCTTTTGGTGTATCTATTGTATTCTTATTATTATGA", "aa": "MKSLNTSIFGGMVIGIVTAYTFNYFSKIELPPALSFFSGIRLVPIILVPISFGVSIVFLLL", "aa_hexdigest": "3096ce78a6fd002e495bc430cc4f19ad", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6654.0108999999975, "isoelectric_point": 9.522705650329591 }, "id": "OAIKOHHJFB_937", "locus": "GCA000209735_00918" }, { "type": "cds", "sequence": "contig_1", "start": 512926, "stop": 513219, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCCTATTGCAATTTTACCTATTGCTGGTTTATTATTAGGAATCGGTGGAGCTATTGGTGCAAATACAACTAATAGTGCTGGTCAAACAACTGCTAATATTTTCAAAGGTGTATCTGAAGTTATTTTTGGAAACTTACCTATTTTATTCTGTATTGCCATAGCGATCACATTTTCAAAAGACAAAGGTGGAGCAGGATTCTCGGCTGCTATTGCTTACCTTGTCTTTACTAGTTCTCAATTAGCATTCATCCAATTTGATGGTGTTGATATTGTTAAAAGTATTTTTTGA", "aa": "MLPIAILPIAGLLLGIGGAIGANTTNSAGQTTANIFKGVSEVIFGNLPILFCIAIAITFSKDKGGAGFSAAIAYLVFTSSQLAFIQFDGVDIVKSIF", "aa_hexdigest": "cae89d5cef978e68e85658c77fc48a9f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9895.530799999991, "isoelectric_point": 5.916791343688964 }, "id": "OAIKOHHJFB_938", "locus": "GCA000209735_00919" }, { "type": "cds", "sequence": "contig_1", "start": 513550, "stop": 514296, "strand": "+", "frame": 1, "gene": "cutC", "product": "PF03932 family protein CutC", "db_xrefs": [ "KEGG:K06201", "RefSeq:WP_013526898.1", "SO:0001217", "UniParc:UPI0001F5ABFD", "UniRef:UniRef100_C4XF03", "UniRef:UniRef50_A0A3P8LIN9", "UniRef:UniRef90_C4XF03" ], "nt": "ATGAAAAATATTGTTACAGAAGTAGCTGTTGATTCATATCAATCAGCATTAAATGCAGATTTAGCTAAAGCCAAAAGAGTTGAACTTTGCTCAGACTTAGTGTTAGGAGGCTTAACTCCTTCGGTTGGTTTATATAACTTAGTGAGAAAAAATACTAAGCTTGATATTGTAGTTATGATTAGACCAAGGTCAGGAGATTTTTGTTATAGTGCTAGTGAAATAGAACATATTATTGAAGATATTAAAGTATTTAGCAGTTTAAAAGCAGATGCTTTAGTTGTCGGAATTTTAAATGGTGACTTTAGCATTAATTTAGAAGCTATGAAAAAAGTAATAAAAGCTGCTAAAAATACTCCAATAGTATTTCATCGAGCTTTTGATGTAGTTAATGATCAATTTAAAGAATTAGAAAATCTTAAAAGACTTGGAGTTAAAAGAATTTTAACTTCAGGAGCTAAACCAAATTGTGATTTAGGTCTCGAAAGAATCAAAGAATTAGTAAAAGTAGCTAATGGCGAAATAACTATTATGCCAGGAGCTGGAGTTAATGCTAATAATGCTGATAAAATAGTGCAAGAAACTAAAGCTACTGAAATTCACTTTACTGCTAAAAGCATAGTAAATACAAGTAGTTTGGACAAAAGTAAGGAAGTAAATTATTCTTCAAAAGAATTAAATGAACATCTAAAAATTGAAAGTGAAGTAGCTAAAATTACTGAAATAATTGATAAGATTAAAGCAGCTTAA", "aa": "MKNIVTEVAVDSYQSALNADLAKAKRVELCSDLVLGGLTPSVGLYNLVRKNTKLDIVVMIRPRSGDFCYSASEIEHIIEDIKVFSSLKADALVVGILNGDFSINLEAMKKVIKAAKNTPIVFHRAFDVVNDQFKELENLKRLGVKRILTSGAKPNCDLGLERIKELVKVANGEITIMPGAGVNANNADKIVQETKATEIHFTAKSIVNTSSLDKSKEVNYSSKELNEHLKIESEVAKITEIIDKIKAA", "aa_hexdigest": "111ce63fb4a9eedea60cd8d3f8d47cd4", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001F5ABFD", "ncbi_nrp_id": "WP_013526898.1", "uniref100_id": "UniRef100_C4XF03", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5ABFD", "RefSeq:WP_013526898.1", "UniRef:UniRef100_C4XF03" ] }, "ips": { "uniref100_id": "UniRef100_C4XF03", "uniref90_id": "UniRef90_C4XF03" }, "psc": { "uniref90_id": "UniRef90_C4XF03", "product": "PF03932 family protein CutC", "uniref50_id": "UniRef50_A0A3P8LIN9", "kegg_orthology_id": "K06201" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LIN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LIN9" ], "product": "PF03932 family protein CutC" }, "genes": [ "cutC" ], "id": "OAIKOHHJFB_939", "locus": "GCA000209735_00920" }, { "type": "cds", "sequence": "contig_1", "start": 514528, "stop": 514647, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGAAAAAAAAGAAACAGCAGTTCAAGAAACTGCAAAAGTTGAAAAAAAATTTATCGTTTCTAAAGATAAATTATTAGAAGCTGGTACATATTTTGGCCACAAAGCAAGTATGTGA", "aa": "MSEKKETAVQETAKVEKKFIVSKDKLLEAGTYFGHKASM", "aa_hexdigest": "acea58cda84d9cc5bb1a24e6c4432506", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4389.0560000000005, "isoelectric_point": 9.10114688873291 }, "id": "OAIKOHHJFB_940", "locus": "GCA000209735_00921" }, { "type": "cds", "sequence": "contig_1", "start": 514657, "stop": 514875, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGAATTCTTATTACCACAACCAAAACGTGGAGTACATGTTATTAACTCAAACTTCACATTACAAAGACTTGAATTTATTTACAACTTATTGAATAAATTCTTAGCAAAAAATCCACATGCGTCATTTATTTTTGTTGGTACAAAAAAACAAGCACGCGAAACAATTAAAGATAATGCTTTAAGAATAAATAGCTACTATGTTACTGAAAGATGA", "aa": "MKEFLLPQPKRGVHVINSNFTLQRLEFIYNLLNKFLAKNPHASFIFVGTKKQARETIKDNALRINSYYVTER", "aa_hexdigest": "610e9e4f4734bb1e96dbed18d9de0d5b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8511.816199999997, "isoelectric_point": 10.163263511657714 }, "id": "OAIKOHHJFB_941", "locus": "GCA000209735_00922" }, { "type": "cds", "sequence": "contig_1", "start": 514927, "stop": 515520, "strand": "+", "frame": 1, "gene": "rpsB", "product": "30S ribosomal protein S2", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015935", "KEGG:K02967", "SO:0001217", "UniRef:UniRef50_A5IY48", "UniRef:UniRef90_C4XF04" ], "nt": "ATGGAAGAATTAGAAGCTAAAGCTGCTAAAGGTTTTGAAGGTTACACTAAAAAAGAAGGTCTTTTATTCCAAAAGAAATTAGACAAACTTCACAAAAACCTTGAAGGTATTCGTCGTATGCAAGGACTTCCAACATTTATGATTGTTGCAGATCCTAATGATGACGAAATTGCTGTTAAGGAAGCACGTAAAAAAGGTGTAAAAATTATTGGTATTTTAGACTCAAATGCTAACCCAGATTTAGTAGACTTTGGTATTCCTGCTAATGATGATTCAGCTAAAAGTATTACATTAATTATTACAATTTTAGCTGATGCTATTGCAACAGCTAGAGGTGGTAGAGCTCGTTTTGCTTTCCAACCTGATGAAAAAATTGTTTTACCAGAATTCAAAAGTGAAAGAAAAGAAAACAATTTTGTAAGAAGACCATTTAACCATAACAGAAACGAAAATCATGAAGCCGGTGAAAGAAAAGCTCACCATTCAGAAAATCATGAACAAGAAGTTAAAGAAGAAAAATCTTCAAAAGGCAAAAAACAGTCAGAAGAAGTAGTTGCTAAAAAACCAGATACTAAAAAAACTGCAACTAAATAG", "aa": "MEELEAKAAKGFEGYTKKEGLLFQKKLDKLHKNLEGIRRMQGLPTFMIVADPNDDEIAVKEARKKGVKIIGILDSNANPDLVDFGIPANDDSAKSITLIITILADAIATARGGRARFAFQPDEKIVLPEFKSERKENNFVRRPFNHNRNENHEAGERKAHHSENHEQEVKEEKSSKGKKQSEEVVAKKPDTKKTATK", "aa_hexdigest": "87e883a310d404affcc9da91bef69281", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "rpsB" ], "truncated": "5-prime", "pseudogene": { "start": 514627, "stop": 515520, "observations": { "mutation": [ 514645, 514873 ], "stop": [ 514645, 514873 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF04", "query_cov": 1.0, "subject_cov": 0.5969696969696969, "identity": 1.0, "score": 324.0, "evalue": 2.85e-109, "gene_start": 1, "gene_end": 197, "reference_start": 134, "reference_end": 330, "reference_sequence": "MSEKKETAVQETAKVEKKFIVSKDKLLEAGTYFGHKASMWNPRMKEFLLPQPKRGVHVINSNFTLQRLEFIYNLLNKFLAKNPHASFIFVGTKKQARETIKDNALRINSYYVTERWLGGTLTNSSTIFKRVKTMEELEAKAAKGFEGYTKKEGLLFQKKLDKLHKNLEGIRRMQGLPTFMIVADPNDDEIAVKEARKKGVKIIGILDSNANPDLVDFGIPANDDSAKSITLIITILADAIATARGGRARFAFQPDEKIVLPEFKSERKENNFVRRPFNHNRNENHEAGERKAHHSENHEQEVKEEKSSKGKKQSEEVVAKKPDTKKTATK" }, "paralog": false, "identity": 0.9932659932659933, "score": 526.0, "evalue": 4.52e-191, "description": "Internal stop codon at 514645, 514873. Nonsense mutation around 514645, 514873.", "psc": { "uniref90_id": "UniRef90_C4XF04", "gene": "rpsB", "product": "30S ribosomal protein S2", "uniref50_id": "UniRef50_A5IY48", "kegg_orthology_id": "K02967", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF04", "UniRef:UniRef50_A5IY48", "KEGG:K02967", "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_A5IY48", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IY48" ], "product": "Small ribosomal subunit protein uS2" } }, "id": "OAIKOHHJFB_942", "locus": "GCA000209735_00923" }, { "type": "cds", "sequence": "contig_1", "start": 515529, "stop": 515618, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAATTAGGTTTAATTAAAGAATTACGTGAACGTACCAATGGTGGTATGGTTGACTGTAAAAAAGCTTTAGAAGAATCAAACTGA", "aa": "MDKLGLIKELRERTNGGMVDCKKALEESN", "aa_hexdigest": "3f533d98b1c752413aa9c3129e34b77a", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3278.778800000001, "isoelectric_point": 6.088445091247558 }, "id": "OAIKOHHJFB_943", "locus": "GCA000209735_00924" }, { "type": "cds", "sequence": "contig_1", "start": 515739, "stop": 516401, "strand": "+", "frame": 3, "gene": "tsf", "product": "translation elongation factor Ts", "db_xrefs": [ "GO:0003746", "GO:0006414", "SO:0001217", "UniRef:UniRef50_A5IY49", "UniRef:UniRef90_C4XF05" ], "nt": "ATGGTTGAATTAAACTGTGAAACAGACTTTGTTGCACGTAATGAAAAATTCAAAGCTTTAGTAGAAAAAATTGCTAAATTAATTTTAGATTCTAAAGCTAAAACAGTTGAAGCTGCTCTTAAATTAAAAGATGGTAAAGAAACTGTTAACTCATTATGTGAAAATTTAACAGCAACAATTGGCGAAAAAATTACACTTCGTCGTTTTGAAATTGTTGAAGCTAAAAAAGGTGAAATTTTAGGTCACTTTGTTCACGTTAATGGTCAAATCGCTGCTATTGTTAAAGTAGCAGGTAGTCATGAAGAAAGTGCAAGAAATGTTGCAATGCACTTAAGTGCTATGAAACCAGAATTTATTTTTGCTAAAGATATTCCTGCAAGAAGACTTCAATTATTCAAAGATGAATTTGTAGTTCCAGCAAACTTTGACAAAAAACCAGAAAAAGTTCAAGAAATGATTCGTCAAGGATCACTTAACAAAAAAATTGGTGAAGTTACTTTAGAAGAACAACCATTTATGATGGATGAAAGTTTAACAATCAATAAATACTTAGCTAACCATAAAAGTAAATTAGTTTCAGCTATTCGTTATGGTGTAGGTGAAGGTATTGAAAAAGTTGCTTGTGACTTTGCTTCAGAAGTTGCTGCGCAAATGAAAAAATAA", "aa": "MVELNCETDFVARNEKFKALVEKIAKLILDSKAKTVEAALKLKDGKETVNSLCENLTATIGEKITLRRFEIVEAKKGEILGHFVHVNGQIAAIVKVAGSHEESARNVAMHLSAMKPEFIFAKDIPARRLQLFKDEFVVPANFDKKPEKVQEMIRQGSLNKKIGEVTLEEQPFMMDESLTINKYLANHKSKLVSAIRYGVGEGIEKVACDFASEVAAQMKK", "aa_hexdigest": "da67cf1959e64fb9ca5ad5025aa9f57e", "start_type": "ATG", "rbs_motif": null, "genes": [ "tsf" ], "truncated": "5-prime", "pseudogene": { "start": 515517, "stop": 516401, "observations": { "mutation": [ 515616, 515640 ], "stop": [ 515616, 515640 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF05", "query_cov": 1.0, "subject_cov": 0.7482993197278912, "identity": 1.0, "score": 416.0, "evalue": 9.07e-146, "gene_start": 1, "gene_end": 220, "reference_start": 75, "reference_end": 294, "reference_sequence": "MGVTMDKLGLIKELRERTNGGMVDCKKALEESNWDIEKAITWLKSNGKIKAAKKANRVSAEGLVAIAANATKAVMVELNCETDFVARNEKFKALVEKIAKLILDSKAKTVEAALKLKDGKETVNSLCENLTATIGEKITLRRFEIVEAKKGEILGHFVHVNGQIAAIVKVAGSHEESARNVAMHLSAMKPEFIFAKDIPARRLQLFKDEFVVPANFDKKPEKVQEMIRQGSLNKKIGEVTLEEQPFMMDESLTINKYLANHKSKLVSAIRYGVGEGIEKVACDFASEVAAQMKK" }, "paralog": false, "identity": 0.9897959183673469, "score": 556.0, "evalue": 3.92e-203, "description": "Internal stop codon at 515616, 515640. Nonsense mutation around 515616, 515640.", "psc": { "uniref90_id": "UniRef90_C4XF05", "gene": "tsf", "product": "translation elongation factor Ts", "uniref50_id": "UniRef50_A5IY49", "go_ids": [ "GO:0003746", "GO:0006414" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF05", "UniRef:UniRef50_A5IY49", "GO:0003746", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_A5IY49", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IY49" ], "product": "Elongation factor Ts" } }, "id": "OAIKOHHJFB_944", "locus": "GCA000209735_00925" }, { "type": "cds", "sequence": "contig_1", "start": 516863, "stop": 517162, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF06", "UniRef:UniRef90_C4XF06" ], "nt": "ATGGAAAATGGTGATAAAAAACCAATTACATTAGCTAAAGGAATTGATTTAAGCACAATTTTCAAAATTAATTTCCATGAACTTATAGAAAAGGTGGGACATAAATTAAATCAATTGCCGGCATTACCTTATTTAGGCTTAAAAAATCAATTGGAATTTGTTAACAGGATAACAGAAAACATTAACGAGGAAACTAAAGCCAATGCACCACGAATTGTTATAATTCGCAGAAGAGCAACTCTAAATGATTTTGATGTTAGAGAATGTAAAAGAAAATTAAATCAAGTTCAAGACTTTTAA", "aa": "MENGDKKPITLAKGIDLSTIFKINFHELIEKVGHKLNQLPALPYLGLKNQLEFVNRITENINEETKANAPRIVIIRRRATLNDFDVRECKRKLNQVQDF", "aa_hexdigest": "76153966046da841818a9a276aaf90d7", "start_type": "ATG", "rbs_motif": "AGGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 516632, "stop": 517162, "observations": { "mutation": [ 516803 ], "stop": [ 516803 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF06", "query_cov": 1.0, "subject_cov": 0.5625, "identity": 1.0, "score": 199.0, "evalue": 6.23e-64, "gene_start": 1, "gene_end": 99, "reference_start": 78, "reference_end": 176, "reference_sequence": "MMETKITTSNEQLPILKPILLASDFVEKIKGDKLYSDVMMLIAQDEDYVYFVSCLKEWEKNYELEPRFIKIYEEEGIMENGDKKPITLAKGIDLSTIFKINFHELIEKVGHKLNQLPALPYLGLKNQLEFVNRITENINEETKANAPRIVIIRRRATLNDFDVRECKRKLNQVQDF" }, "paralog": false, "identity": 0.9886363636363636, "score": 345.0, "evalue": 9.87e-124, "description": "Internal stop codon at 516803. Nonsense mutation around 516803.", "psc": { "uniref90_id": "UniRef90_C4XF06", "uniref50_id": "UniRef50_C4XF06", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF06", "UniRef:UniRef50_C4XF06" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF06", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF06" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11499.206599999983, "isoelectric_point": 9.413754081726076 }, "id": "OAIKOHHJFB_945", "locus": "GCA000209735_00926" }, { "type": "cds", "sequence": "contig_1", "start": 517257, "stop": 517376, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAGTATTTTAGCTTTAGATAATACTTTGGATAATAAAATTATGAAAGCCAAAAATGCTGATGAAATTTATGAAGTAATGAGTAAATTTAATGTTGATATGCACCAATACGAATAA", "aa": "MISILALDNTLDNKIMKAKNADEIYEVMSKFNVDMHQYE", "aa_hexdigest": "969d29bc32388b7cc542439509c441fe", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4577.236600000001, "isoelectric_point": 4.780352592468262 }, "id": "OAIKOHHJFB_946", "locus": "GCA000209735_00927" }, { "type": "cds", "sequence": "contig_1", "start": 517464, "stop": 517736, "strand": "-", "frame": 3, "gene": null, "product": "PTS EIIA type-2 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF07", "UniRef:UniRef90_C4XF07" ], "nt": "ATGAAATTCAATAATTTTTTAAACAAAAATTCTATTTTTGTACATTCAAATTTTGATAAAAAAGATGATGTTTTGCACTTTTTATCACATCAACTAAATGAACAAAACTTTGTTAGTGATGAAAAGAAAATATATGAAGCTTTTTTATATAGAGAAGAATTAATGAATACTGCAATAGGCGGTGGCATTGCAATTTGTCAAGCATTAAGCTCAACAGTTAAAAAAGACACATTATGTATTTTAACTATGAATAATGATGGGGTTAATGATTGA", "aa": "MKFNNFLNKNSIFVHSNFDKKDDVLHFLSHQLNEQNFVSDEKKIYEAFLYREELMNTAIGGGIAICQALSSTVKKDTLCILTMNNDGVND", "aa_hexdigest": "00845ba209b2df49145b0c4e31cd8a4c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 517257, "stop": 517778, "observations": { "mutation": [ 517466 ], "stop": [ 517466 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF07", "query_cov": 1.0, "subject_cov": 0.5202312138728323, "identity": 1.0, "score": 181.0, "evalue": 7.9e-57, "gene_start": 1, "gene_end": 90, "reference_start": 15, "reference_end": 104, "reference_sequence": "MRKKRNLIIERTTKMKFNNFLNKNSIFVHSNFDKKDDVLHFLSHQLNEQNFVSDEKKIYEAFLYREELMNTAIGGGIAICQALSSTVKKDTLCILTMNNDGVNDWKADDEKNVNLVFALVLSKNNRNLQIDTLQMISILALDNTLDNKIMKAKNADEIYEVMSKFNVDMHQYE" }, "paralog": false, "identity": 0.9884393063583815, "score": 339.0, "evalue": 1.72e-121, "description": "Internal stop codon at 517466. Nonsense mutation around 517466.", "psc": { "uniref90_id": "UniRef90_C4XF07", "product": "PTS EIIA type-2 domain-containing protein", "uniref50_id": "UniRef50_C4XF07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF07", "UniRef:UniRef50_C4XF07" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF07" ], "product": "PTS EIIA type-2 domain-containing protein" } }, "id": "OAIKOHHJFB_947", "locus": "GCA000209735_00928" }, { "type": "cds", "sequence": "contig_1", "start": 517925, "stop": 518170, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAATAAAACTTAATTATTTGATTGAGGGCGGTAGAGTTAATAATAGCTTTAAAAAATTCGTTTTAAAAACAATGTCTAAATTAAGAATTTATCCTTATGTTAAGTTTATTAAACAACATGTTTCAACAGCTGGAATAGCTCGTGGTGTTAAGGGTGCAATAATTCAAATATTTAAAAAATTAGGAGCTTTCGTTAAAGCAAAAGATCTCAAAACATTAACAGAAAACTTCAAAATTGACTGA", "aa": "MPIKLNYLIEGGRVNNSFKKFVLKTMSKLRIYPYVKFIKQHVSTAGIARGVKGAIIQIFKKLGAFVKAKDLKTLTENFKID", "aa_hexdigest": "4667e27c91505db3ca2c179c5788b992", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9217.032699999992, "isoelectric_point": 10.371818733215331 }, "id": "OAIKOHHJFB_948", "locus": "GCA000209735_00929" }, { "type": "cds", "sequence": "contig_1", "start": 518291, "stop": 518638, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATAGGTTTTATTGTTGACGGCTTTATTAGTGAACCGCAAGAACTAATTCTTAAAAAAGGTTATGGTTATTTTCCTTTTTTAACAGATATTGATGGTGTAGTTTATAAAGATGGTATTGACTTAGACCAAGAAGATTTATTGCATAAACTTGATCAAGGAAAATCAGTTAAATCATCTATGCCAAATGTTAAAGCTGTTTTAGATACAGTGGACAAAATGACTAAAGAATATGATGAAGTAATTTACTTCCCAATTGGTTCATCATTATCTTCAAGTTGCAACAACATTGCAACAATTTGCAAAGAATATAAAAACTTTCACGTTTATGATAACTGCTGA", "aa": "MKKIGFIVDGFISEPQELILKKGYGYFPFLTDIDGVVYKDGIDLDQEDLLHKLDQGKSVKSSMPNVKAVLDTVDKMTKEYDEVIYFPIGSSLSSSCNNIATICKEYKNFHVYDNC", "aa_hexdigest": "b035d9d7828fa00fd8885e999c2c6109", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13014.75079999999, "isoelectric_point": 4.889824485778808 }, "id": "OAIKOHHJFB_949", "locus": "GCA000209735_00930" }, { "type": "cds", "sequence": "contig_1", "start": 518831, "stop": 519256, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAATTGTGAAGAAGATGAAAACATTATTTTGGAAACAAATGAATATGACTTAATTAATTTAAATACATATGATCGACCAAAAACAACTTTATTAATTAAACCATTACAAAACGGATTTAATTATCGATTAATTAACAATGCTTTTAGTAATTTGAAAGAAGGCATTAACTTAGGTTTATATATCGAAAAGATAAATGACCGTTTAACTAACTATGTTAATATCGGAAATATCAAGGTTTTTGTTATATCGGAAAAGATTTGCAAATACATTGAAAAAGATCAAATTTTATACATAAAACTTTTAAACTTCGTTAAAACAATTAGCACTATTAAAAATCATGTTATTATTTATCAAAACTTACCAGAAGGCTTAGATGAACTTATGGTAAAAACATTAGATGTACAATATGTTTGCAAATAG", "aa": "MENCEEDENIILETNEYDLINLNTYDRPKTTLLIKPLQNGFNYRLINNAFSNLKEGINLGLYIEKINDRLTNYVNIGNIKVFVISEKICKYIEKDQILYIKLLNFVKTISTIKNHVIIYQNLPEGLDELMVKTLDVQYVCK", "aa_hexdigest": "a9dbe22fda2d6b8cf452d580def35fcf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16537.991999999987, "isoelectric_point": 5.228698539733886 }, "id": "OAIKOHHJFB_950", "locus": "GCA000209735_00931" }, { "type": "cds", "sequence": "contig_1", "start": 519296, "stop": 519457, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGATTATGAAAAGAAAAATGAATTGCAACAATATATAAAAGAAAACATTCCAATTATTTCATATAAAACTAAACGTCAAAGCAAAGTTGCAATTTTAAAAAATAGAGTCATAGGTTACTCTGACCAAGATTCAGAATTCTTTAATAATGTTTCGTGA", "aa": "MLDYEKKNELQQYIKENIPIISYKTKRQSKVAILKNRVIGYSDQDSEFFNNVS", "aa_hexdigest": "2770d6336b0c9917ac49cd5717d50604", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6328.123400000001, "isoelectric_point": 9.003799629211429 }, "id": "OAIKOHHJFB_951", "locus": "GCA000209735_00932" }, { "type": "cds", "sequence": "contig_1", "start": 519509, "stop": 519877, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTTATGCTTTGGGCATCAATGAATTTCTCTACAATAATGTCATTAGTAAATCAGACATTGAAGATATTTTGAGAATATTTGAGCTTAATAATATGTTTGGATATTACTACGTTAAAAACGGATTAATATTATTAATCTTTGAAACCAATTCGTCATTCTGGCTAAACCACTACACTAAAAAAGCACTTAAAAGATTAAAATTCTTACATGAAAGAAAAATTATTAATCCATTTTTCAATAGCTTTGCTTTTTTAAGTGTTAACACTTTCAAAAGTGTTGATGATGTTCAAGAATTAGTTGATAAATCAAAATACTTAATACAACACTTAAATAACAAACGCAAATTTGATAAATATTCTTCTTGA", "aa": "MAYALGINEFLYNNVISKSDIEDILRIFELNNMFGYYYVKNGLILLIFETNSSFWLNHYTKKALKRLKFLHERKIINPFFNSFAFLSVNTFKSVDDVQELVDKSKYLIQHLNNKRKFDKYSS", "aa_hexdigest": "9ce520f26f5d313bc2eb115ff611fbaf", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14595.680999999973, "isoelectric_point": 9.371076011657713 }, "id": "OAIKOHHJFB_952", "locus": "GCA000209735_00933" }, { "type": "cds", "sequence": "contig_1", "start": 519971, "stop": 520447, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF46DBA", "UniRef:UniRef90_UPI000FF46DBA" ], "nt": "ATGGATCAATTATTATTGGGAATAGCAATTATAATAGCATGTGTTATTTTAATAGACATCTTTATTATAGTTTTTATTTACTTAAATCATGTTCATAAAGTCAAAACAAATGGTTTAGCAATCTTTAAATTAGACTTTGGACAAAAAAAAATAATTCGCTTGAGTTCTAAAAGCTTAAGTGGATCTTTAAACATGGACTCACGTCTTCAAGGTCTTTCAGCTAATCAATATGTCGACTTTGATGTCTTTCTTTCTTACTTAGATGAAGAGAGCCAAAAAAACTTGACTGAATATCTTTCACAAAACGACAATAATGATAAAAGATTTAATATTTACTGCAAGATGAGTTCTAAAGGCTATCGAACACATCAAAATGCTCAAGGTTTAAAACTTCTAAACATTAAAATGACTAAAGAAAAAATTCTTTTAAAATTCTTCCCTCTTGGAAATAATCGATACTACTTAACAATCCACTGA", "aa": "MDQLLLGIAIIIACVILIDIFIIVFIYLNHVHKVKTNGLAIFKLDFGQKKIIRLSSKSLSGSLNMDSRLQGLSANQYVDFDVFLSYLDEESQKNLTEYLSQNDNNDKRFNIYCKMSSKGYRTHQNAQGLKLLNIKMTKEKILLKFFPLGNNRYYLTIH", "aa_hexdigest": "7982d92f1d45b1c475cf241714921b50", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI000FF46DBA", "query_cov": 1.0, "subject_cov": 0.8540540540540541, "identity": 0.9940000000000001, "score": 297.0, "evalue": 1.9e-101, "valid": true, "uniref50_id": "UniRef50_UPI000FF46DBA" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF46DBA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF46DBA" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18272.16730000001, "isoelectric_point": 9.394155693054199 }, "id": "OAIKOHHJFB_953", "locus": "GCA000209735_00934" }, { "type": "cds", "sequence": "contig_1", "start": 520491, "stop": 520994, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13361.12", "PFAM:PF13538.12" ], "nt": "ATGACAGTCCACTCTGCTAAAGGATTAGAATTTGACAATATCTTTTTAGTGGGAATGAGTGATCAAATTTTCCCTAACTATAAATCATTAAAATCAAACTCAGATGATGCTTTAGAAGAAGAAAGAAGACTTGCTTATGTGGCAATTACCAGAGCAAGAAATAGATTATTTATTAGCGATAGTCGTGGATATTTAATTGGTACAGATTTAGAAAAGAAACCATCACGTTTTATTCGTGAAATGGGAATTGAACTTAAAAACTTTATTCTTAAAACAGGTTCTGTTGGCCTTGATTTTGATGAAATAGTTGACGAAAAACAAATAAAAGCTTTGAACAAAAAAATTATAGTAGGTGACATTATTAGCCACACCTTCTTTGGCGAAGGCCAAGTTGTGGCTGTTGAAAACGATACAATTAGCGTTAGATTCACAGGACAAAAAACAATCAAAGTTTTAGCTAAAAATCACGCTTCAATTCGACTTTTAAAAAATAGAGATGATTAA", "aa": "MTVHSAKGLEFDNIFLVGMSDQIFPNYKSLKSNSDDALEEERRLAYVAITRARNRLFISDSRGYLIGTDLEKKPSRFIREMGIELKNFILKTGSVGLDFDEIVDEKQIKALNKKIIVGDIISHTFFGEGQVVAVENDTISVRFTGQKTIKVLAKNHASIRLLKNRDD", "aa_hexdigest": "40f03f5eca9a4eaaa1fba12918512889", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13361.12", "name": "UvrD_C", "length": 61, "aa_cov": 0.3652694610778443, "hmm_cov": 0.9672131147540983, "evalue": 1.6338739566557974e-26, "score": 89.57945251464844, "start": 1, "stop": 61 }, { "id": "PF13538.12", "name": "UvrD_C_2", "length": 23, "aa_cov": 0.1377245508982036, "hmm_cov": 1.0, "evalue": 5.893831174374378e-11, "score": 38.21773147583008, "start": 36, "stop": 58 } ], "seq_stats": { "molecular_weight": 18907.475199999997, "isoelectric_point": 8.77661304473877 }, "id": "OAIKOHHJFB_954", "locus": "GCA000209735_00935" }, { "type": "cds", "sequence": "contig_1", "start": 521097, "stop": 521651, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13361.12" ], "nt": "GTGGTCCAAAAAATTAATGAATTAAAGAAACAAAAAATTCAGCTTAAAAATATTGCTATTTTTTACCGTTCAAATTATTATTCGCGTCCTTTCGAAGAAGAATTAATTAATGAAAATATTAACCACAAGATTTTTAATGGTCAAAAATTCTTCCAAAGAACTGAAGTTAAGAATGCTCTAGCATTTTTAAGAGTTATCTATGATGGCTCTGATGTTTCATTAAATAGAATAATTAATGTTCCAAACCGTAAAATCGGAGAAGCAACTCTTAAAAAATTACAAGAAGCAGCAAATAATAAAAAATTAACTCTCTATTCATATTTAATTAAATATTACAAGGAATTGCCAGTACCTTTAAATGTTAAAAACAACATTGTTAAGTTTCTTAACAAAATCAACCTTTATCGTCAAGCTTTAAAAAGCAACTCAATTAGTGTTGTTTTAGATAAATTCTTAAAGGAAATTGGCTACTATGAACATATAGAAGAAGAAACCTCACTTCGTGGCACTGCTAAAGATAATGTTGCTGAATTAATTCGCTCAATAGTAACTTGA", "aa": "MVQKINELKKQKIQLKNIAIFYRSNYYSRPFEEELINENINHKIFNGQKFFQRTEVKNALAFLRVIYDGSDVSLNRIINVPNRKIGEATLKKLQEAANNKKLTLYSYLIKYYKELPVPLNVKNNIVKFLNKINLYRQALKSNSISVVLDKFLKEIGYYEHIEEETSLRGTAKDNVAELIRSIVT", "aa_hexdigest": "5e395766e0c7a5ec03084fa9c6a9d23f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13361.12", "name": "UvrD_C", "length": 109, "aa_cov": 0.5869565217391305, "hmm_cov": 1.0, "evalue": 3.4171544239774456e-24, "score": 81.94786834716797, "start": 2, "stop": 109 } ], "seq_stats": { "molecular_weight": 21590.796600000016, "isoelectric_point": 9.696447944641115 }, "id": "OAIKOHHJFB_955", "locus": "GCA000209735_00936" }, { "type": "cds", "sequence": "contig_1", "start": 521652, "stop": 521861, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13361.12" ], "nt": "TTGATCCTTGATTTTGATAAGGATTTCAAGAATACTAAAACGATTATTTTAAATACAAATTACCGCTCAACTAAAACAATTTTAGATGCAGCTAACAAATTAATCAAACACAACAAAAAACGTTTTAGTAAAGATTTAATAACAAATCGTGAACAAGGAACACCAATAGAATTTATTCATGCTTTTAGTGTCGAAGCTGAAGCTAGATGA", "aa": "MILDFDKDFKNTKTIILNTNYRSTKTILDAANKLIKHNKKRFSKDLITNREQGTPIEFIHAFSVEAEAR", "aa_hexdigest": "eed357a029a74435c1ec55ba62ef2466", "start_type": "TTG", "rbs_motif": "AGGAG/GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13361.12", "name": "UvrD_C", "length": 55, "aa_cov": 0.7971014492753623, "hmm_cov": 1.0, "evalue": 1.8137236011622613e-11, "score": 40.09764099121094, "start": 15, "stop": 69 } ], "seq_stats": { "molecular_weight": 8054.158399999998, "isoelectric_point": 9.62907848358154 }, "id": "OAIKOHHJFB_956", "locus": "GCA000209735_00937" }, { "type": "cds", "sequence": "contig_1", "start": 522216, "stop": 522662, "strand": "-", "frame": 3, "gene": null, "product": "UvrD-helicase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002204F9A8" ], "nt": "ATGAGCAAACGAGATGAAATTTTGAATTCATTAAATGCTGAACAAAAAGAAGCTTTAATGTATTTTGATGGTCCTTTAAGAATCATCGCTGGAGCTGGAAGTGGTAAAACTAGAGTTTTAACTAGGAAGGTGGCTTATTTAATTAATGAACTTGGAATTAGCCCACGTTCAATTTTAGCAGTGACTTTCACTAATAAAGCTGCGAACGAAATGAGCGAACGTGTTAAACAATACGTCGGCGAAGAAGCTAATCAAATCGATATTTGTACTTTCCACTCTTTAAGCTCAAAGATTTTAAGGAAGGAAGCTATTCATGTTAATTTAAGTAATGACTTCCAAATTATTGATGAAACAGATAAGAAGACAATTATTGCTCAACTTTATAAAGCTGAAGGCATTTCAAATGAAGATGTTTCATACAAAAATGTTATGAAGATTTTCTCATGA", "aa": "MSKRDEILNSLNAEQKEALMYFDGPLRIIAGAGSGKTRVLTRKVAYLINELGISPRSILAVTFTNKAANEMSERVKQYVGEEANQIDICTFHSLSSKILRKEAIHVNLSNDFQIIDETDKKTIIAQLYKAEGISNEDVSYKNVMKIFS", "aa_hexdigest": "3ac20ae09ec7741bfea9f24769d48083", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_UPI002961EF37", "query_cov": 0.8513513513513513, "subject_cov": 0.9197080291970803, "identity": 0.508, "score": 139.0, "evalue": 6.76e-40, "valid": false, "product": "UvrD-helicase domain-containing protein", "uniref50_id": "UniRef50_UPI002204F9A8" }, "pscc": { "uniref50_id": "UniRef50_UPI002204F9A8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002204F9A8" ], "product": "UvrD-helicase domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_957", "locus": "GCA000209735_00938" }, { "type": "cds", "sequence": "contig_1", "start": 522675, "stop": 522833, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAATGGCTTTAAGAACCAGACTTGGACTTTTTGATCGTTCAACAAACACCAGAGTAACATACTTAAAACATTACTCACAATCAAGCTATTTAGATGAAATCAAACTGTTACCATTCAAAAGTAATAATGAAGCTGTAATTTACATTAAAAAATAG", "aa": "MEMALRTRLGLFDRSTNTRVTYLKHYSQSSYLDEIKLLPFKSNNEAVIYIKK", "aa_hexdigest": "24259b18c6c6dda4e02fcec5cf01e20e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6184.107499999999, "isoelectric_point": 9.695158576965333 }, "id": "OAIKOHHJFB_958", "locus": "GCA000209735_00939" }, { "type": "cds", "sequence": "contig_1", "start": 523002, "stop": 523223, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTCCATAAAGGTGTTGTCAAGGCAATTAACTTTTATTCTTTTATTATATTTTCAACAGTTATTTTACTTGTGCTATTTTTACTAATTCCATTGTTTTATAGTAGTAGAACAATTGGAATGCTGATTTTTAGAGTACAAATAATACCTGCTAAAAAGATGACTCCTTTAGCTTTAAAATTATTACCTTTAAGAAGAAATGCATTTGCTTTAATTTTCTGA", "aa": "MVHKGVVKAINFYSFIIFSTVILLVLFLLIPLFYSSRTIGMLIFRVQIIPAKKMTPLALKLLPLRRNAFALIF", "aa_hexdigest": "364b28c26d48461e1e7a845902056279", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8381.402699999991, "isoelectric_point": 11.614382362365724 }, "id": "OAIKOHHJFB_959", "locus": "GCA000209735_00940" }, { "type": "cds", "sequence": "contig_1", "start": 523961, "stop": 524341, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00535.33" ], "nt": "ATGAAATTATCAATTATTTCACCAGGAATAAATAGCGCTAAAAGTTTAAAAAATATTTTAGAAGGTATGAAAGATCAAAATAATCAAGATTTTGAATTAATTTTAGCTTTAAGAAACCCTTCAGCTAAAATGTATTCTATTATTGAAAGATATTTAGCATTCTTTGGCACACGTTTAAAATTTATTACTAATAATCGTCAGAGAACTATGCAAAGTGATATAGTTTGTGCTTTTCACTTAGTTAAAGGAAACTATGCCACAATTATTAATAGTGACAACTCAATGCGTTCTTACTTTGCACAAGAATTAACAAACTATATTGAAAAATTTAATCCAGATGTTTTAGAATATCGTCCAAGATTGGTTGGCACAGTTAAATGA", "aa": "MKLSIISPGINSAKSLKNILEGMKDQNNQDFELILALRNPSAKMYSIIERYLAFFGTRLKFITNNRQRTMQSDIVCAFHLVKGNYATIINSDNSMRSYFAQELTNYIEKFNPDVLEYRPRLVGTVK", "aa_hexdigest": "93ef7331a216a15f14767417a79719b9", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00535.33", "name": "Glycos_transf_2", "length": 111, "aa_cov": 0.8809523809523809, "hmm_cov": 0.990990990990991, "evalue": 4.4835840731634195e-11, "score": 39.0565185546875, "start": 4, "stop": 114 } ], "seq_stats": { "molecular_weight": 14546.66419999998, "isoelectric_point": 9.586658287048337 }, "id": "OAIKOHHJFB_960", "locus": "GCA000209735_00941" }, { "type": "cds", "sequence": "contig_1", "start": 524351, "stop": 524485, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "TTGCGTCGTTTTGGCGATAGTGGTTCAAGCGGTTTTGGAGTTGGTTTTGAAAGATTAGTAATGTATGTCACAGGTGTTGACAATATTAGAGATGTTATTCCATATCCAAGAACATCTGGAAATATTAAAATGTAA", "aa": "MRRFGDSGSSGFGVGFERLVMYVTGVDNIRDVIPYPRTSGNIKM", "aa_hexdigest": "3d7ca7e8900fcfe6bdb806abfc6963ef", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A378BFT2", "query_cov": 0.9318181818181818, "subject_cov": 0.8541666666666666, "identity": 0.659, "score": 60.8, "evalue": 1.55e-11, "valid": false, "product": "Asparaginyl-tRNA synthetase", "uniref50_id": "UniRef50_A0A378BFT2" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 36, "aa_cov": 0.8181818181818182, "hmm_cov": 1.0, "evalue": 6.352188191360074e-11, "score": 37.924766540527344, "start": 3, "stop": 38 } ], "seq_stats": { "molecular_weight": 4883.5452000000005, "isoelectric_point": 9.684585762023925 }, "id": "OAIKOHHJFB_961", "locus": "GCA000209735_00942" }, { "type": "cds", "sequence": "contig_1", "start": 524495, "stop": 525010, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGATTGAGCCTGAAGTTGCTTTCTATGAATTAAAGGATGCAATCAAATTAGCTGATGACATGTTAAAATCTGTTATCAAAAATACAATTAAAAAACATCCTGCTGAATTTAAATTTTTAAATGAAACAGTTGACAAAGGTCTTTTAGAAAGATTAAATCACTTTTTAAAAACAAAACTTAGTGTTATTGACTATCGCGACGCAATTAAAGAATTGGCTAAAGTTAAAGACCAATTTGAAAATCAAGATATTGAATTCGGACTTGATTTAGCAACTGAACATGAAAGATATATTTCAGAAAAAATCTTTAATGGACCAGTAGCTGTAATTAATTTTCCAAAAAGTTTTAAAGCATTCTATATGCAACAAAATGATGACAATGAAACTGTAGCAGCTTTTGACTTATTAGTGCCAGGAATTGGCGAATTAATAGGTGGAAGCCAAAGAGAAGTTAGATATGACAAATTACTTCAAAGAGCTTTAGAAGTAGGAATCAGTCAAGAAGAATTGCAATGA", "aa": "MIEPEVAFYELKDAIKLADDMLKSVIKNTIKKHPAEFKFLNETVDKGLLERLNHFLKTKLSVIDYRDAIKELAKVKDQFENQDIEFGLDLATEHERYISEKIFNGPVAVINFPKSFKAFYMQQNDDNETVAAFDLLVPGIGELIGGSQREVRYDKLLQRALEVGISQEELQ", "aa_hexdigest": "27ce7c518c470dca834d2a40a8868e05", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 178, "aa_cov": 0.9883040935672515, "hmm_cov": 0.8089887640449438, "evalue": 3.0561668558485853e-21, "score": 71.81175231933594, "start": 1, "stop": 169 } ], "seq_stats": { "molecular_weight": 19666.273599999986, "isoelectric_point": 5.000831031799316 }, "id": "OAIKOHHJFB_962", "locus": "GCA000209735_00943" }, { "type": "cds", "sequence": "contig_1", "start": 525011, "stop": 525307, "strand": "-", "frame": 1, "gene": null, "product": "amino acid--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B779D" ], "nt": "ATGTTAATAAGATCAACGTTAGCTAAAGAAGTTAATAAATATTTTGATGAACACGGTTACTTATACTTCAATGCTCCTATTATTACCAGCAATGATGGTGAAGGAGCTGGAGAAACTTTTGTAGTTAATGATGGAAATTCAAAAGATCCTTTTTTTGGGACTAGAAAAGCTACTTTAGGAGTTACAGGACAATTGCATGCTGAAAGTTATGCAATTGGATTTAAAAAAGTTTATACTTTTGCACCTACTTTTAGAGCAGAACACTCAAATACCAAAAAACATGCAGCAGAATTTTGA", "aa": "MLIRSTLAKEVNKYFDEHGYLYFNAPIITSNDGEGAGETFVVNDGNSKDPFFGTRKATLGVTGQLHAESYAIGFKKVYTFAPTFRAEHSNTKKHAAEF", "aa_hexdigest": "c8911058b0c3505beaa206642a212cb9", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00387B779D", "query_cov": 0.8775510204081632, "subject_cov": 0.8514851485148515, "identity": 0.674, "score": 116.0, "evalue": 4.51e-32, "valid": false, "product": "amino acid--tRNA ligase-related protein", "uniref50_id": "UniRef50_UPI00387B779D" }, "pscc": { "uniref50_id": "UniRef50_UPI00387B779D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B779D" ], "product": "amino acid--tRNA ligase-related protein" }, "genes": [], "id": "OAIKOHHJFB_963", "locus": "GCA000209735_00944" }, { "type": "cds", "sequence": "contig_1", "start": 525999, "stop": 526328, "strand": "+", "frame": 3, "gene": null, "product": "PQ-loop repeat-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF44E35", "UniRef:UniRef90_UPI000FF44E35" ], "nt": "ATGGAAATAGCTCAATATGTTTTTGGAACTTTGGCTAGTTTATTAATGGTTTCAATCTGCATACCTCAAATTATCCAAACTCTAAAAACCAAAAGTGTTGGAAATGTTGCATATTCAACATTCATTATTTACTTTTTTGGTGGATTTATTTTTGTGCTTACTATGTTATTAAAAGATGGGGTTGGAACTTATGGTTTAAGCGATCCCCTTGTTAATATAATTGGTAATGTTACATTTGCAATTTTAATGGCCTTTACTATTACTTTATTCTTTATATATGATAAAAAAACTAATAAAGTTTTTAAAATTGGTATTGGTTCATTACTTTGA", "aa": "MEIAQYVFGTLASLLMVSICIPQIIQTLKTKSVGNVAYSTFIIYFFGGFIFVLTMLLKDGVGTYGLSDPLVNIIGNVTFAILMAFTITLFFIYDKKTNKVFKIGIGSLL", "aa_hexdigest": "b37ea1e873c200715c04adc101b217a4", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 525999, "stop": 526538, "observations": { "mutation": [ 526326, 526413 ], "stop": [ 526326, 526413 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000FF44E35", "query_cov": 1.0, "subject_cov": 0.5989010989010989, "identity": 1.0, "score": 205.0, "evalue": 5.96e-66, "gene_start": 1, "gene_end": 109, "reference_start": 1, "reference_end": 109, "reference_sequence": "MEIAQYVFGTLASLLMVSICIPQIIQTLKTKSVGNVAYSTFIIYFFGGFIFVLTMLLKDGVGTYGLSDPLVNIIGNVTFAILMAFTITLFFIYDKKTNKVFKIGIGSLLWILALSGLVFFIVVYANKNARTNLGPDNGWMIAMSVIATCCCALPFTIQIVKTIKSKSADGLSLPMLYLGKLY" }, "paralog": false, "identity": 0.9888268156424581, "score": 342.0, "evalue": 2.06e-122, "description": "Internal stop codon at 526326, 526413. Nonsense mutation around 526326, 526413.", "psc": { "uniref90_id": "UniRef90_UPI000FF44E35", "product": "PQ-loop repeat-containing protein", "uniref50_id": "UniRef50_UPI000FF44E35", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000FF44E35", "UniRef:UniRef50_UPI000FF44E35" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000FF44E35", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF44E35" ], "product": "PQ-loop repeat-containing protein" } }, "id": "OAIKOHHJFB_964", "locus": "GCA000209735_00945" }, { "type": "cds", "sequence": "contig_1", "start": 526416, "stop": 526601, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04193.19" ], "nt": "ATGATAGCGATGTCTGTTATTGCAACTTGTTGTTGTGCTTTACCTTTTACAATTCAAATTGTGAAAACAATTAAAAGTAAAAGCGCGGATGGTCTTAGCTTACCAATGCTTTATTTAGGAATTGTATTAAATGCATTATTATGTATTTATTTAGGACTAGTAGTTCCATTTAATACAGCTACTTGA", "aa": "MIAMSVIATCCCALPFTIQIVKTIKSKSADGLSLPMLYLGIVLNALLCIYLGLVVPFNTAT", "aa_hexdigest": "ebb8bc4c1a04248b81aee1cce0420332", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04193.19", "name": "PQ-loop", "length": 49, "aa_cov": 0.8032786885245902, "hmm_cov": 1.0, "evalue": 9.344917590874885e-09, "score": 31.071535110473633, "start": 3, "stop": 51 } ], "seq_stats": { "molecular_weight": 6462.937399999999, "isoelectric_point": 8.45897731781006 }, "id": "OAIKOHHJFB_965", "locus": "GCA000209735_00946" }, { "type": "cds", "sequence": "contig_1", "start": 526837, "stop": 527043, "strand": "+", "frame": 1, "gene": null, "product": "Aminopeptidase E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEW7", "UniRef:UniRef90_A0A3P8MEW7" ], "nt": "ATGCAAATTAATAAAAAAGAAATTAATAATTTATATAAAAAATATTTAAAAAATTCAAACAATAAATTAATAGAAAGCGCTGTTACTAAAAATGGTATTTTCAATGCAAGTTATAACAATGACATGCAAACAAAACATAATTTTGAATTTAATACAGAAGTAGCTAAAGCTGGAATGACTAATCAAAAAAGTTCAGGACGTTGCTGA", "aa": "MQINKKEINNLYKKYLKNSNNKLIESAVTKNGIFNASYNNDMQTKHNFEFNTEVAKAGMTNQKSSGRC", "aa_hexdigest": "a730b0eb2dd06b5c996edae0430fcb1b", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 526837, "stop": 527146, "observations": { "insertion": [ 527092 ], "mutation": [ 527041 ], "stop": [ 527041 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8MEW7", "query_cov": 1.0, "subject_cov": 0.6666666666666666, "identity": 0.8240000000000001, "score": 114.0, "evalue": 1.6e-31, "gene_start": 1, "gene_end": 68, "reference_start": 1, "reference_end": 68, "reference_sequence": "MKIDKKEINKFYKKYAKNSTNKLIESAMTKNGIFNASYNNDMQVKHNFEFNTQVTKTGITNQKSSGRCWIFSGLNMLKVKASKALMLKILNSAKTTFYFETN" }, "paralog": false, "identity": 0.7941176470588235, "score": 161.0, "evalue": 6.86e-53, "description": "Internal stop codon at 527041. Frameshift due to insertion around 527092. Nonsense mutation around 527041.", "psc": { "uniref90_id": "UniRef90_A0A3P8MEW7", "product": "Aminopeptidase E", "uniref50_id": "UniRef50_A0A3P8MEW7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8MEW7", "UniRef:UniRef50_A0A3P8MEW7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MEW7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEW7" ], "product": "Aminopeptidase E" } }, "id": "OAIKOHHJFB_966", "locus": "GCA000209735_00947" }, { "type": "cds", "sequence": "contig_1", "start": 527308, "stop": 527778, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTGAAACATTTAACTCAAGCAATACTCACGGTTTAAATACTGCATTAAATTGTCAATTAATTAGTGCGACAAAAGAAATTAGAGAAGCTATTAAAAATAAAGCTAGTCAAGAAGTTTTAATGAAAATTAAACATGAAGCTCTTGAAAGAATTTTTGAAATCAATGCTAAATCATTAGGTTTACCTCCTCAAAAATTTTCATTTGAATATCGTGATAAATATAAAAAATTTATCAGAATCGAAAATGTAACACCATTAGAATTCTTAGAAAAACATGTAGGTACAGATTTTCTTAATAAAGTTAATTTATTAGATGATCCTCGTAAAGAAAATCCTAAAAATAGATTGCTTGTAGCTAAATACTACAAATCAGTTATCGAAGAAAAAGGATTAGAGTCACTTAATGTTACAATGGATGTAATTAAAAAAGCTGTTATTGCTTCATTAAAAGATAATGAGCCAGTTTGA", "aa": "MPETFNSSNTHGLNTALNCQLISATKEIREAIKNKASQEVLMKIKHEALERIFEINAKSLGLPPQKFSFEYRDKYKKFIRIENVTPLEFLEKHVGTDFLNKVNLLDDPRKENPKNRLLVAKYYKSVIEEKGLESLNVTMDVIKKAVIASLKDNEPV", "aa_hexdigest": "68f7ecb7d4464509bad7743f4c82ce3c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17932.56199999999, "isoelectric_point": 9.059758186340332 }, "id": "OAIKOHHJFB_967", "locus": "GCA000209735_00948" }, { "type": "cds", "sequence": "contig_1", "start": 527854, "stop": 527976, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGAATATTAAAAATACTTTAACTAAAGAAGATAGAGTTAATTATCGTCTTTCAGTTCAAACTCATGCTATGACAATTGTTGGTGTTAATTTAGATAAAGATAATAATCCAATTAATTGA", "aa": "MLNIKNTLTKEDRVNYRLSVQTHAMTIVGVNLDKDNNPIN", "aa_hexdigest": "2b0d6b4aa31f0a9e3ef043cbe2504b00", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4584.1962, "isoelectric_point": 8.210580635070801 }, "id": "OAIKOHHJFB_968", "locus": "GCA000209735_00949" }, { "type": "cds", "sequence": "contig_1", "start": 528275, "stop": 528376, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGCTAAAGTTATTAAAACAATAGCTCAAGTTTTAGTTGTATTATCACTATTATTTATCTGTTCACTTGCTATTTTTAACATGCAAGCAGGACACTGA", "aa": "MTAKVIKTIAQVLVVLSLLFICSLAIFNMQAGH", "aa_hexdigest": "5ee581c4bc7dae510991c297905e9406", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3574.4103999999998, "isoelectric_point": 9.305705070495605 }, "id": "OAIKOHHJFB_969", "locus": "GCA000209735_00950" }, { "type": "cds", "sequence": "contig_1", "start": 528665, "stop": 528808, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAAACGTGCAAAGTTAATTACAGCTAATATTAAACCTGTGACAAAACCGATTAAGGTTTCTTTTCAAATAACTTTACCATATTCACCAGGTTCTATTTCTTTTAAAGCTAAGGCTCGATTGACTGAAATATTAGCTTGA", "aa": "MPKRAKLITANIKPVTKPIKVSFQITLPYSPGSISFKAKARLTEILA", "aa_hexdigest": "e981b0f201ed9c55f48b7cd127fac8e0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5168.235, "isoelectric_point": 10.79015407562256 }, "id": "OAIKOHHJFB_970", "locus": "GCA000209735_00951" }, { "type": "cds", "sequence": "contig_1", "start": 529003, "stop": 529596, "strand": "-", "frame": 2, "gene": "mgtE", "product": "magnesium transporter MgtE N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C659EE" ], "nt": "ATGAAACTACTACAAGAATTACAAAGTGATGAACTAGCTGATGTTTTAGAAGAATTACCTTCGAATGTCACAAGCAAAATCTTAGCTTATACACCTCAAGAAAAAAGAGCTGAATTAAATAAACTTTTAAGCTATGAAGATGATGAAGTTGGAAGCATAATGTCAATAGACATTTCTTCAATTGGTAATTCATATACTTGCGAACAAGCTTTACATAAAATTAGACGTGATTATCATAAGAAAAATGCTGAATTAGTGCATTACTATTACGTAGTTAATGAAACAAATAAACTAATTGGTGCTTTAACACTAGAAGAAATAGTTTTTGCTGATCCTAATGCTAAAATTGATGATATTTATAATCCTGTAACTTATGTTTCAGCTCATGATAAAACCGAAGTAGCAGCCCAAGTTTTTAGTGAACACGATATGTCAGTATTACCTGTAGTTAACCATGAAAAAAGATTAGTTGGAATGATTACAAGTGACGACGTTATTGATGTTATTAAAGAAGAAGCTACAGAAGATTTATACAAAATGGCAGGTATTAGTAGCCATGAAGTTAATGAAAGTGAATACTTAAAAACTCCATGA", "aa": "MKLLQELQSDELADVLEELPSNVTSKILAYTPQEKRAELNKLLSYEDDEVGSIMSIDISSIGNSYTCEQALHKIRRDYHKKNAELVHYYYVVNETNKLIGALTLEEIVFADPNAKIDDIYNPVTYVSAHDKTEVAAQVFSEHDMSVLPVVNHEKRLVGMITSDDVIDVIKEEATEDLYKMAGISSHEVNESEYLKTP", "aa_hexdigest": "b69a6432d78c5459c90f2ae14c5c1d39", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI0024C659EE", "query_cov": 0.9137055837563451, "subject_cov": 0.8411214953271028, "identity": 0.522, "score": 177.0, "evalue": 6.02e-53, "valid": false, "product": "magnesium transporter MgtE N-terminal domain-containing protein", "uniref50_id": "UniRef50_UPI0024C659EE" }, "pscc": { "uniref50_id": "UniRef50_UPI0024C659EE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024C659EE" ], "product": "magnesium transporter MgtE N-terminal domain-containing protein" }, "genes": [ "mgtE" ], "id": "OAIKOHHJFB_971", "locus": "GCA000209735_00952" }, { "type": "cds", "sequence": "contig_1", "start": 529600, "stop": 529851, "strand": "-", "frame": 2, "gene": null, "product": "Magnesium transporter", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KNN3" ], "nt": "ATGAGCGAAAGTCAAGAGATTAGTTTGCAAAACGAACTACATGAAATTATTAAAACTAAAAATGTAACTAAAGCCAGAGAATTTATTGATAATAATCCAATGGCTGATGTTGCAAACTTATTAAATGAATTACAGTTAGAAGAACAGCTAACTTTTTTGCCTTTACTTAAAACTAGTGAAGCTGCTGAATTATTTAGCTACCTTGATGAAGAAGTCCAAGCTAATTTAGCTCAAAGTTTTACAGAAGATTGA", "aa": "MSESQEISLQNELHEIIKTKNVTKAREFIDNNPMADVANLLNELQLEEQLTFLPLLKTSEAAELFSYLDEEVQANLAQSFTED", "aa_hexdigest": "e79bd320d1434f37e735e5c9a4e1b022", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_A0A3P8KNN3", "query_cov": 1.0, "subject_cov": 0.8645833333333334, "identity": 0.892, "score": 136.0, "evalue": 3.59e-40, "valid": false, "product": "Magnesium transporter", "uniref50_id": "UniRef50_A0A3P8KNN3" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KNN3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KNN3" ], "product": "Magnesium transporter" }, "genes": [], "id": "OAIKOHHJFB_972", "locus": "GCA000209735_00953" }, { "type": "cds", "sequence": "contig_1", "start": 529853, "stop": 530434, "strand": "-", "frame": 1, "gene": null, "product": "DAC domain-containing protein", "db_xrefs": [ "EC:2.7.7.85", "KEGG:K18672", "RefSeq:WP_013354684.1", "SO:0001217", "UniParc:UPI0001A8C272", "UniRef:UniRef100_C4XF17", "UniRef:UniRef50_L0RXD2", "UniRef:UniRef90_C4XF17" ], "nt": "ATGTGTTTAGAATCTAAAATCGGTTTTTATGTAATTGTTGCATTATTAGTTACTTTAATTTTAATTTCAATAATGCCACTTATAGTTAATGCAATTAAAAACAAAATGGCTAGAAGTCATTTCCAAAAATTAGGTCAAAGTAGTCAAATTAGATTAATTAACCAACTACGTGAAGCTGTTGAATATTTATCAAAAAATAAAATTGGAGCTTTAATTACAATTGAAAACAATGATAATATTGACAATTTGAGAACTGATGGGGTAATTTTAAATGCTAATATCAGTAGTAGCTTACTTATTTCAATATTCAATAAATATAGTCCATTGCATGATGGAGCTGTAATTATAAGAGATAATAAAATTTATTATGCTTCTACTTTTTATAAAATCACCAGAAAATCAATTGATAATCAATATGGTGCTCGTCATAGAGCTGCTATGGGAATTAGTGAACTTTGTGATGCTACCACAATAATTGTTAGTGAAGAAACAGGGGATGTTAAATTTGTTAAAAATGGTACTTTCTACAACATTCGTCTTGAACAATTCCAAGAACAACTAGTTAAATACTTAAAGGATTAA", "aa": "MCLESKIGFYVIVALLVTLILISIMPLIVNAIKNKMARSHFQKLGQSSQIRLINQLREAVEYLSKNKIGALITIENNDNIDNLRTDGVILNANISSSLLISIFNKYSPLHDGAVIIRDNKIYYASTFYKITRKSIDNQYGARHRAAMGISELCDATTIIVSEETGDVKFVKNGTFYNIRLEQFQEQLVKYLKD", "aa_hexdigest": "fd947db9bc030b75f4722be4eed0c1e6", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C272", "ncbi_nrp_id": "WP_013354684.1", "uniref100_id": "UniRef100_C4XF17", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C272", "RefSeq:WP_013354684.1", "UniRef:UniRef100_C4XF17" ] }, "ips": { "uniref100_id": "UniRef100_C4XF17", "uniref90_id": "UniRef90_C4XF17" }, "psc": { "uniref90_id": "UniRef90_C4XF17", "product": "DAC domain-containing protein", "ec_ids": [ "2.7.7.85" ], "uniref50_id": "UniRef50_L0RXD2", "kegg_orthology_id": "K18672" }, "pscc": { "uniref50_id": "UniRef50_L0RXD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RXD2" ], "product": "DAC domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_973", "locus": "GCA000209735_00954" }, { "type": "cds", "sequence": "contig_1", "start": 530444, "stop": 530746, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013526910.1", "SO:0001217", "UniParc:UPI0001F5AC06", "UniRef:UniRef100_C4XF18", "UniRef:UniRef50_C4XF18", "UniRef:UniRef90_C4XF18" ], "nt": "ATGGCAATTATTGATAGAGATCTAGCAACATGTGCTGAAAGAATTCAAAAAAGAAGAGAATTACTATTAAAACTTTATAATGAAAATCAAGCTGAATTAGCTAATCCAGCTTTGATTGAAGATGAAAAACCTATTACTGGTGAATGCCAAGAATTTATGTGTTTAGTAAATAAACATGAGAAAGAAGATCCTAGAGTTAGAAAGCAAAAAATTATTCTTAAAGTTTTAGGAGCTAGCTTATCAGTTTTAATTATTTTACTTTGCACACTACTAGCTGTTTTAATTTCAAGAGTTTATGCTTAG", "aa": "MAIIDRDLATCAERIQKRRELLLKLYNENQAELANPALIEDEKPITGECQEFMCLVNKHEKEDPRVRKQKIILKVLGASLSVLIILLCTLLAVLISRVYA", "aa_hexdigest": "6ab1b92ccf6a12287af4e5fdad6c5583", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001F5AC06", "ncbi_nrp_id": "WP_013526910.1", "uniref100_id": "UniRef100_C4XF18", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5AC06", "RefSeq:WP_013526910.1", "UniRef:UniRef100_C4XF18" ] }, "ips": { "uniref100_id": "UniRef100_C4XF18", "uniref90_id": "UniRef90_C4XF18" }, "psc": { "uniref90_id": "UniRef90_C4XF18", "uniref50_id": "UniRef50_C4XF18" }, "pscc": { "uniref50_id": "UniRef50_C4XF18", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF18" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11402.467599999987, "isoelectric_point": 7.644867134094238 }, "id": "OAIKOHHJFB_974", "locus": "GCA000209735_00955" }, { "type": "cds", "sequence": "contig_1", "start": 531021, "stop": 531383, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021ACBAA4", "UniRef:UniRef90_UPI0021ACBAA4" ], "nt": "TTGTATTTAAATGAAAGAAAAAAAGAAAAATTAAAAATCGATCCTTTTTATGAAAAAAATCATTTTGATGCAAATTCATATTTAATTGAAAAATTATCGAATTTTAAAAATAATTTTATTTACACAATTTCTATCGTTATAAAAGAAAATTCAGAAGAAAATCAATTATATATAAAGAATATCAAAAAACAGATAGACGAAATTAACAAAAAATTATTTGATAGCGAAAAAACATATTTTAAATTCTTTTATCTAAAAACAATTTTTGTCAAAGCTAAAAAACATTTTAAATTATTTACTAAGAAAATAACAAATAAAAGAAATTTGAAATTAAAAAAATTAAATATTGTTAATGAAAAATAG", "aa": "MYLNERKKEKLKIDPFYEKNHFDANSYLIEKLSNFKNNFIYTISIVIKENSEENQLYIKNIKKQIDEINKKLFDSEKTYFKFFYLKTIFVKAKKHFKLFTKKITNKRNLKLKKLNIVNEK", "aa_hexdigest": "9a39b435a8f3ed486c19c0add26b8843", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 531021, "stop": 531572, "observations": { "mutation": [ 531440, 531455, 531539 ], "stop": [ 531440, 531455, 531539 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0021ACBAA4", "query_cov": 1.0, "subject_cov": 0.6557377049180327, "identity": 0.992, "score": 223.0, "evalue": 9.59e-73, "gene_start": 1, "gene_end": 120, "reference_start": 64, "reference_end": 183, "reference_sequence": "MKLTFYYLDSFWNFALFLLPIILALFLNNEIPVLKTFNKWVILFWITFVYLTVSFVRIFFKKDLYLNERKKEKLKIDPFYEKNHFDANSYLIEKLSNFKNNFIYTISIVIKENSEENQLYIKNIKKQIDEINKKLFDSEKTYFKFFYLKTIFVKAKKHFKLFTKKITNKRNLKLKKLNIVNEK" }, "paralog": false, "identity": 0.9781420765027322, "score": 352.0, "evalue": 7.17e-126, "description": "Internal stop codon at 531440, 531455, 531539. Nonsense mutation around 531440, 531455, 531539.", "psc": { "uniref90_id": "UniRef90_UPI0021ACBAA4", "uniref50_id": "UniRef50_UPI0021ACBAA4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0021ACBAA4", "UniRef:UniRef50_UPI0021ACBAA4" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0021ACBAA4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021ACBAA4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 14756.17439999997, "isoelectric_point": 9.8444673538208 }, "id": "OAIKOHHJFB_975", "locus": "GCA000209735_00956" }, { "type": "cds", "sequence": "contig_1", "start": 531603, "stop": 531854, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATATTAGTATAGTAAAAAAAATTTTGGTAGAAAATAAAAACAATTTATTTATTATTTTTCAATGGAAAAATGAACATTTTAATATTAAATTACAAAACAAATTTAAAATTATAGATTTTATTAGGAATAGTTGTGAAGATATAGAATTTTTTAGTTTTTTTGATGCTTATTGTGAAAAAAATGATGAATCAAATATTGATTTACATGAGATATTAGGCGCGAAATATAATAAAAAAATTTGGTTTTGA", "aa": "MDISIVKKILVENKNNLFIIFQWKNEHFNIKLQNKFKIIDFIRNSCEDIEFFSFFDAYCEKNDESNIDLHEILGAKYNKKIWF", "aa_hexdigest": "c46cf596eefbbe7fecec248100533b47", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10140.582799999991, "isoelectric_point": 6.076167869567871 }, "id": "OAIKOHHJFB_976", "locus": "GCA000209735_00957" }, { "type": "cds", "sequence": "contig_1", "start": 532712, "stop": 532876, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTAAAGAGGAATTTTTCAAAGGCGTAAGTAGACCAGTAAATGAAAAATTACAAAAAATTTTTATGCAATTAGATCTAGTTGAACAAACTGGATATGGAGTGCCTTTAATTGTCAAAAAATATGACGAAGAAGCATTTGAAATTGATGAAAATACAATTTGA", "aa": "MTKEEFFKGVSRPVNEKLQKIFMQLDLVEQTGYGVPLIVKKYDEEAFEIDENTI", "aa_hexdigest": "42fc4fc0a2a96e81ee9b060082f65a0a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6339.2024, "isoelectric_point": 4.609210395812987 }, "id": "OAIKOHHJFB_977", "locus": "GCA000209735_00958" }, { "type": "cds", "sequence": "contig_1", "start": 532982, "stop": 533671, "strand": "-", "frame": 1, "gene": null, "product": "Putative ATP-dependent DNA helicase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6GYZ7", "UniRef:UniRef90_Q6GYZ7" ], "nt": "ATGCTCAATAAAAGAGGAACGGGAACAATTTATTTTAGTCTAAAAGATGATCATACAATTGTTGACTTAGATATTTGTAATAAATCCATGAGAGATATTTCTCAAGCTATTTCAAACAATGTTAAGCCAGAAATAATGCCTAGAATTTCGATTGAATTTATTGATAACCAAAATGTAATTAAAGTTGAAGCTGAAGGAGAACAAAAACCTTATTCAGCTTATGATAAATATTATATTCGTATTGGAGAAGAAGATAAAAAACTTAATAGAGATAAATTAGTAGATTTTGTGCAAAAATCAGTTTTTAAAATAAATATTTGTGAAATTGAAAACAAAGATCAAGATTTGACATTTAATAGTTTTTTGCTATATGCTAATATTAAAAATTTAACAGTAAATAAAGATACTTTCGAAAAAAATTTTGATTTAAAGACTAAAAATAATAAATACAATTTAATGTCTTATTTATTATCAAATAATAATGATATTTCAATTAAAGTTAATACTTTTAAAGATAATGATAAAAGTGTTTTGTTGAAAAGAAACGAATATGGAAATAAATGTTTATTTTATGCAATTGAGAGCATTTTAAATTATGTAGGTTCTATTAATGATACTTATGTAGATTTGTCAACTGCAACCAGGAGTAAACAAAAATTATTCGATTTTGAGTGTTTTAGAGAAGCATGA", "aa": "MLNKRGTGTIYFSLKDDHTIVDLDICNKSMRDISQAISNNVKPEIMPRISIEFIDNQNVIKVEAEGEQKPYSAYDKYYIRIGEEDKKLNRDKLVDFVQKSVFKINICEIENKDQDLTFNSFLLYANIKNLTVNKDTFEKNFDLKTKNNKYNLMSYLLSNNNDISIKVNTFKDNDKSVLLKRNEYGNKCLFYAIESILNYVGSINDTYVDLSTATRSKQKLFDFECFREA", "aa_hexdigest": "4cbfdae80d914b527267de72a2777514", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 532679, "stop": 533759, "observations": { "insertion": [ 533699 ], "mutation": [ 533744 ], "stop": [ 532714, 532957, 532984, 533744 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q6GYZ7", "query_cov": 1.0, "subject_cov": 0.5264367816091954, "identity": 0.93, "score": 422.0, "evalue": 9e-146, "gene_start": 1, "gene_end": 229, "reference_start": 30, "reference_end": 258, "reference_sequence": "MNKMPWESEEVEYKKSTSELKEAIISLTAMLNKKGTGTVYFGVKDDRTVVGLEIGNKTMRDISQAISNNVKPEIMPRISIEFIDNQNVIKVEAEGEQKPYSAYDKYYIRIGEEDKKLNRDKLVDFVQKSVSKINICEIENKDQDLTFNSFLLYANIKNLTVNKDTFEKNFDLKTKNNKYNLMSYLLADNNDISIKVNTFKDNDKSVLLKRNEYGNKCLFYAIESVLNYVGSINDTYVDLSTATRSEQKLFNFECFREAWLNACIHNRWDEKYPPQVNIFKDYIQIESNGGIPRNMTKEKFFKGVSRPVNEKLQKIFMQLDLVEQTGHGVPLIVKKYGEEAFEIDENTIWVKIPFNRKGFKRINEKENKNNLNDNQIKVIKTIEKESNITINQLSSTLSLSEGYIKKIINQLKNKNLIERNGLRKNGNWTIISYNL" }, "paralog": false, "identity": 0.9220055710306406, "score": 654.0, "evalue": 1.55e-239, "description": "Internal stop codon at 532714, 532957, 532984, 533744. Frameshift due to insertion around 533699. Nonsense mutation around 533744.", "psc": { "uniref90_id": "UniRef90_Q6GYZ7", "product": "Putative ATP-dependent DNA helicase", "uniref50_id": "UniRef50_Q6GYZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q6GYZ7", "UniRef:UniRef50_Q6GYZ7" ] }, "pscc": { "uniref50_id": "UniRef50_Q6GYZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6GYZ7" ], "product": "Putative ATP-dependent DNA helicase" } }, "id": "OAIKOHHJFB_978", "locus": "GCA000209735_00959" }, { "type": "cds", "sequence": "contig_1", "start": 533969, "stop": 534151, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAAATATTTAAATATATGCCAGCTAGAATTAAGTTTTTTGCTTTTCTAAGTGCCTTTTTTGCTATCTTACAACCTTTTTTATCAATGCTTAATCCTACTATTACAAAACAATTAATTACTTTGATAGCTAAAGTTCAAAATGGTACTAGTATGAATGAAGTAAGTTTACTATTTTGA", "aa": "MLKIFKYMPARIKFFAFLSAFFAILQPFLSMLNPTITKQLITLIAKVQNGTSMNEVSLLF", "aa_hexdigest": "add83737200df4372843180430a2d7d3", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6888.356699999998, "isoelectric_point": 10.294907951354979 }, "id": "OAIKOHHJFB_979", "locus": "GCA000209735_00960" }, { "type": "cds", "sequence": "contig_1", "start": 534182, "stop": 534457, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTATTTAATAATTATGGCCATAGGTTTTGCATTTTTGTTAATGATTGTTGCTTTTCTTAGTGCATTCTTTGCTGCTAGAACTTGTATTTATGGAACTTATTATTTAAGAAAAAATTTGTTTGAGCATCTTTTAACTCTTTCAAGAAGCAATTTAGAGAAATATTCAGTTGGTAGCTTAATTACTCGTTTTAGTAATGACTTAAACAAAATTAAAGATGTGATATTTGTAGTTGTTAGAGGGCTAGTTGTTTCACCTTTCTTTGTAGTTTGA", "aa": "MIYLIIMAIGFAFLLMIVAFLSAFFAARTCIYGTYYLRKNLFEHLLTLSRSNLEKYSVGSLITRFSNDLNKIKDVIFVVVRGLVVSPFFVV", "aa_hexdigest": "c025e796f0cb2ede63367e85fe28e841", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10424.44209999999, "isoelectric_point": 9.727586174011229 }, "id": "OAIKOHHJFB_980", "locus": "GCA000209735_00961" }, { "type": "cds", "sequence": "contig_1", "start": 534767, "stop": 535138, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTTTTGGCAATGTAGCTATTTTTGCCCTAGTAGCCTATTTAATAGGCTCTAAAGGCGATGACAATGTTGCAAAATTAATTGGTGATGTATCACAATTTACAGCGAATTTAAGCATGATTTCAATGGGCGTTTTTAACACACTTTTTACAATTAATAGACTATTTAGATCAAGTGTTTCAGCTAAAAGATATATTGAATTAATGAATTCAAAAAGTGAAATTCCTTTTGTAGTTAGTGATAAAAAAGTAACTAGTGGGGCTATTGAATTTAAGAATGTTAATTTTGCCTATCCTATCTATAAAAAAGGCAAATTGGTCGATACTAAAGAAGCTTTAAAGAATATAAATTTAAAAATTGAAGCAGGCTAA", "aa": "MLFGNVAIFALVAYLIGSKGDDNVAKLIGDVSQFTANLSMISMGVFNTLFTINRLFRSSVSAKRYIELMNSKSEIPFVVSDKKVTSGAIEFKNVNFAYPIYKKGKLVDTKEALKNINLKIEAG", "aa_hexdigest": "ba353abb194dd39e922159023d3b3b8b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13549.713299999981, "isoelectric_point": 9.618570137023926 }, "id": "OAIKOHHJFB_981", "locus": "GCA000209735_00962" }, { "type": "cds", "sequence": "contig_1", "start": 535313, "stop": 535756, "strand": "+", "frame": 2, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF22", "UniRef:UniRef90_C4XF22" ], "nt": "ATGATTATTTCTGGCTCAATTAAAGAAAATATTACTTTTTCAAATCATGAATTAAGTGAACAAGAAATTGAAAAAGCTATTAATATTTCATGTGCCAACTTTATTAAAGAATTAGATGACAAATATGAAGCTAGAGTTGATCAACGTGGTAAAAACTTTAGTGGTGGTCAACGTCAAAGAGTTGCTATTGCTCAAGCTTTAATTAAAAAACCTCAAATTTTAATTTTAGATGATTCAACTAGTGCTCTAGATAATGAAACTGATTTAAGAGTACGTCAAAACATTAAAGAAAACTTAAAAGATAGCACTCTAATAGTAATTAGTCAAAGAATCAATTCAATTAAAGATTCAAATCAAATCTTTGTTCTTGATAAAGGACAAATTGTTGCTAGCGGTAAACACAGTCAACTATTAAACACAAGTTCTATATATAAAGATATATGA", "aa": "MIISGSIKENITFSNHELSEQEIEKAINISCANFIKELDDKYEARVDQRGKNFSGGQRQRVAIAQALIKKPQILILDDSTSALDNETDLRVRQNIKENLKDSTLIVISQRINSIKDSNQIFVLDKGQIVASGKHSQLLNTSSIYKDI", "aa_hexdigest": "bfdff8d3d6f5f95d65ffa0401bc5a134", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 535142, "stop": 535786, "observations": { "mutation": [ 535754 ], "stop": [ 535754 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF22", "query_cov": 1.0, "subject_cov": 0.6869158878504673, "identity": 1.0, "score": 276.0, "evalue": 7.88e-93, "gene_start": 1, "gene_end": 147, "reference_start": 58, "reference_end": 204, "reference_sequence": "MGIIGKTGSGKSTLVKLLTHEYLLDKKDGEIIIDSHNINEIDTNNFYEKVTQVYQKPMIISGSIKENITFSNHELSEQEIEKAINISCANFIKELDDKYEARVDQRGKNFSGGQRQRVAIAQALIKKPQILILDDSTSALDNETDLRVRQNIKENLKDSTLIVISQRINSIKDSNQIFVLDKGQIVASGKHSQLLNTSSIYKDIWQSQEQKVGE" }, "paralog": false, "identity": 0.9906542056074766, "score": 409.0, "evalue": 1.28e-147, "description": "Internal stop codon at 535754. Nonsense mutation around 535754.", "psc": { "uniref90_id": "UniRef90_C4XF22", "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_C4XF22", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF22", "UniRef:UniRef50_C4XF22" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF22", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF22" ], "product": "ABC transporter domain-containing protein" } }, "id": "OAIKOHHJFB_982", "locus": "GCA000209735_00963" }, { "type": "cds", "sequence": "contig_1", "start": 535954, "stop": 536253, "strand": "+", "frame": 1, "gene": null, "product": "ABC transmembrane type-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF23", "UniRef:UniRef90_C4XF23" ], "nt": "ATGGGTTTTATTACTGATAAATTTTTAGATCCTAAAACTTTCAATTTAGCTAATTTTAAAATGAAAGACTATATTGTCTTTGTTGTAATGTTAGCTTTAGTTTATGTCTTTATAAAAATTTTTGAAATTTCGCAAAGAGTTATTGTTTCTAAGCTTAGTGCTCGCCTTGGTCGGAATTTAAGGCTGCAATGCTATCAAAAAATTCAACAAATGGAAATGAGTTTCTATGAAGAAGAACAAACTGGTGACTTAATGTCAGCATTAACTAATGATATTCAAAATATAACTGAAGCAATCTGA", "aa": "MGFITDKFLDPKTFNLANFKMKDYIVFVVMLALVYVFIKIFEISQRVIVSKLSARLGRNLRLQCYQKIQQMEMSFYEEEQTGDLMSALTNDIQNITEAI", "aa_hexdigest": "658ed711dbaeb7c5477cca72093968f8", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 535753, "stop": 536349, "observations": { "mutation": [ 535780, 535891, 535948, 536251 ], "stop": [ 535780, 535891, 535948, 536251 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF23", "query_cov": 1.0, "subject_cov": 0.5, "identity": 1.0, "score": 184.0, "evalue": 1.08e-57, "gene_start": 1, "gene_end": 99, "reference_start": 68, "reference_end": 166, "reference_sequence": "MTISRTKSRWIMPRFSNLRFRQKGEKKISFKIFFKFLSNFQISKSVWIFTVLFGLLVASLTNIASWLMGFITDKFLDPKTFNLANFKMKDYIVFVVMLALVYVFIKIFEISQRVIVSKLSARLGRNLRLQCYQKIQQMEMSFYEEEQTGDLMSALTNDIQNITEAIWDILISVISIIFEFSFITIMMFIYVPLLAAII" }, "paralog": false, "identity": 0.9797979797979798, "score": 362.0, "evalue": 4.33e-130, "description": "Internal stop codon at 535780, 535891, 535948, 536251. Nonsense mutation around 535780, 535891, 535948, 536251.", "psc": { "uniref90_id": "UniRef90_C4XF23", "product": "ABC transmembrane type-1 domain-containing protein", "uniref50_id": "UniRef50_C4XF23", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF23", "UniRef:UniRef50_C4XF23" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF23", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF23" ], "product": "ABC transmembrane type-1 domain-containing protein" } }, "id": "OAIKOHHJFB_983", "locus": "GCA000209735_00964" }, { "type": "cds", "sequence": "contig_1", "start": 536355, "stop": 536594, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCAATTAATGCTTCAGTAATTTTCTTAATTATTAGAAGTAACCAAAAATTCTACATCCAACAACAAGAAAGACTCGGTGAATTTAATGGTTATTTAGAAGAAATAATGGATGCTTTTCCTTTAGTTAACTTGCACCAACAAAAAGAAAATGTCGCTAAAGAATTTGACAAATATTCTCGTGCATTAATTAAACCTGATGCTAGTGCTACTACTAGAATGAATATTGTTTTCCCTTGA", "aa": "MPINASVIFLIIRSNQKFYIQQQERLGEFNGYLEEIMDAFPLVNLHQQKENVAKEFDKYSRALIKPDASATTRMNIVFP", "aa_hexdigest": "0d0b2f224b47c0053bfa35dccfd433aa", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9220.52449999999, "isoelectric_point": 6.555263710021973 }, "id": "OAIKOHHJFB_984", "locus": "GCA000209735_00965" }, { "type": "cds", "sequence": "contig_1", "start": 536688, "stop": 537104, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "TTGAGTGCTGGTGTGGTTATGTCATTTACTCTTTATTTATTTAGAATCAGCGACAATATTTCGTTATTGCTTGAAGTAATTAATGGTATTCAGCATGGGCTTGGATCAATAGTTAGAATTAATAAAATATTAGCTTTAGTGCCTCAATTTGATGAAGTTAAATTAAACGAATTAGACTATAAAACAGGCAAAATCGAATTTAAGAACGTTTCTTTTGCTTATGCTTCTAAACCAGATAAGTTAGTCTTAAAAAACATTAGTTTCACTATTGAAAAAGGTAAAAGCTTAGCTTTAGTTGGCCACACTGGTTGTGGTAAAACAACTATAGCTAAACTACTTTCAAAATTCTATGTACCAACTAGTGGCGACATCTTAATTGATGGCCAAAGTATTCTTAAAACAAAAAGTAAAAGTTGA", "aa": "MSAGVVMSFTLYLFRISDNISLLLEVINGIQHGLGSIVRINKILALVPQFDEVKLNELDYKTGKIEFKNVSFAYASKPDKLVLKNISFTIEKGKSLALVGHTGCGKTTIAKLLSKFYVPTSGDILIDGQSILKTKSKS", "aa_hexdigest": "cfd665868848676fff88c05bfc11cde4", "start_type": "TTG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 52, "aa_cov": 0.37681159420289856, "hmm_cov": 1.0, "evalue": 1.0024147831429254e-16, "score": 57.910335540771484, "start": 83, "stop": 134 } ], "seq_stats": { "molecular_weight": 15094.607899999983, "isoelectric_point": 9.547654914855958 }, "id": "OAIKOHHJFB_985", "locus": "GCA000209735_00966" }, { "type": "cds", "sequence": "contig_1", "start": 537252, "stop": 537617, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter, ATP-binding/permease protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A139QXF8" ], "nt": "ATGCCTGAAAAATTTGATACAGTTTTAAAATCAAATGGTGCTAATTTAAGTGAAGGGCAAAAACAATTAATTGCTATTAGCCGTTCATTAATTGCTTCTAAACCAATTTCAATTTTAGATGAAGCTACTTCTAATATTGACACAATAACAGAATTAAAAGTGCAACATGCAATGAAATACTTAATGAAAGATCGAAGTATGTTAATTATTGCTCACCGTTTAAGTACTATTAAAAATGTTGATGAAATTCTAGTTATTAATGAAGGTGAAATAATTGAACGTGGTAATCATGACCAATTAATGAAACAAAATGGACACTACAAAAAAATCTATTCTCTTGGTTTAAATGATACAAAAATTGATTAG", "aa": "MPEKFDTVLKSNGANLSEGQKQLIAISRSLIASKPISILDEATSNIDTITELKVQHAMKYLMKDRSMLIIAHRLSTIKNVDEILVINEGEIIERGNHDQLMKQNGHYKKIYSLGLNDTKID", "aa_hexdigest": "17e9f0fadd868aeee6f0f1183666f8c9", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A139QXF8", "query_cov": 0.9256198347107438, "subject_cov": 0.9491525423728814, "identity": 0.563, "score": 132.0, "evalue": 7.46e-38, "valid": false, "product": "ABC transporter, ATP-binding/permease protein", "uniref50_id": "UniRef50_A0A139QXF8" }, "pscc": { "uniref50_id": "UniRef50_A0A139QXF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A139QXF8" ], "product": "ABC transporter, ATP-binding/permease protein" }, "genes": [], "id": "OAIKOHHJFB_986", "locus": "GCA000209735_00967" }, { "type": "cds", "sequence": "contig_1", "start": 537654, "stop": 538370, "strand": "-", "frame": 3, "gene": null, "product": "DUF4143 domain-containing protein", "db_xrefs": [ "KEGG:K07133", "SO:0001217", "UniRef:UniRef50_UPI0001E32E7D", "UniRef:UniRef90_UPI0001E32E7D" ], "nt": "ATGAAAGAAGCCAACATTAAAACTAATTATTTGGTAAATGAGTTCAAAAGAACTTATTTAATAGATATAGTTGAAAAAAATAAAATAGAAGATATTGATAACTTAGATGACATAATCAATATTTTAGCTTCTAATATAGGTTCATACACTAATCCTTATAAATTATCATTAACTTTTAAATCAATTAAGAAAACAAACATTTCTGATGCAACAATTAGAAAATATATTGAAAATTTAAAAAATGCTTTCATAATTAATGAAGTCAAAAAATATGATATTAAAGGTAAAAAGTATATTAATGCTTCATACAAATATTATTTTGAAGATATAGGTTTAAGAAATGCTAAAATCAATTTTGCACAAAATGAAGAAAATCATTTAATGGAAAATATCATTTATAATGAATTGCTTTTAAGAGGTTACAATGTTAATGTTGGAATAGTAGAAATAAGACCTAATGCTAATCAGAAAGTTCAATTAGAAGTAGATTTTATTGCTCAAAAAGGTAATCAAAAAGTTTATATTCAATCTTGTTTAAATATGTATGATCAAAATAAAGAAATTCAAGAAAAAAGACCTTTTAAAAACATCAATGATAATTTTAAAAAAATTATTATTACTAAAGATGACCCTATTTTTGATACTGATACTAATGGTTATAAAATTTATGGAATCAAACAATTTTTACTTGATTTAGATATTCTAAATAAAAATTAA", "aa": "MKEANIKTNYLVNEFKRTYLIDIVEKNKIEDIDNLDDIINILASNIGSYTNPYKLSLTFKSIKKTNISDATIRKYIENLKNAFIINEVKKYDIKGKKYINASYKYYFEDIGLRNAKINFAQNEENHLMENIIYNELLLRGYNVNVGIVEIRPNANQKVQLEVDFIAQKGNQKVYIQSCLNMYDQNKEIQEKRPFKNINDNFKKIIITKDDPIFDTDTNGYKIYGIKQFLLDLDILNKN", "aa_hexdigest": "d1d0d9a95fd08e1e89f844b12b228ea6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0001E32E7D", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.971, "score": 445.0, "evalue": 9.39e-158, "valid": true, "product": "DUF4143 domain-containing protein", "uniref50_id": "UniRef50_UPI0001E32E7D", "kegg_orthology_id": "K07133" }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32E7D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32E7D" ], "product": "DUF4143 domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_987", "locus": "GCA000209735_00968" }, { "type": "cds", "sequence": "contig_1", "start": 538413, "stop": 538904, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAATTAAACGGGATATATATTTAGAAAAACTTAAAGAGATGATTGATATACCTCTTATTAAAATAATTTCAGGCTTAAGAAGAAGTGGAAAGTCTTTTTTGTTATTTAATTTATTCAAGGAATATTTAATAAATGAGTTAAAAGTTGATCAAAATCATATTATTGAAATTAATTTAGAAAATCAAGAAAATCATTCTTATTGTGATCCTAATAATTTTTTGGAATTTATAAAAAGTAAAATTAAAGATCGAAAAAAGTATTTTCTTTTAATTGATGAAGTGCAACTTTTAAAAGATTTTGTGCCTGTTTTAAATCAACTTAATAATAATCAACAACTTAATGTTTTTGTCACTGGCTCCAATTCTAAATTCTTAATTAATGATGTAGTTACAGAATTTAGAGGTAGATCGATTTCGCTTCATGTTTTTCCCTTATCTTTCTATGAAATAGCCTCACACAAAGAATTTAAAAACCTTGATGAGTTATGA", "aa": "MVIKRDIYLEKLKEMIDIPLIKIISGLRRSGKSFLLFNLFKEYLINELKVDQNHIIEINLENQENHSYCDPNNFLEFIKSKIKDRKKYFLLIDEVQLLKDFVPVLNQLNNNQQLNVFVTGSNSKFLINDVVTEFRGRSISLHVFPLSFYEIASHKEFKNLDEL", "aa_hexdigest": "060299fe7312b29129bebbc3566ea142", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19323.2052, "isoelectric_point": 6.912951469421387 }, "id": "OAIKOHHJFB_988", "locus": "GCA000209735_00969" }, { "type": "tRNA", "sequence": "contig_1", "start": 539080, "stop": 539164, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(aag)", "amino_acid": "Leu", "anti_codon": "aag", "score": 71.4, "nt": "GCCCGAGTGGTGAAATTGGCAAACACGCTAGACTAAGGATCTAGTGCTTAACGGCTTGCAGGTTCAAGTCCTGTCTCGGGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 539128, 539130 ], "id": "OAIKOHHJFB_23", "locus": "GCA000209735_00970" }, { "type": "cds", "sequence": "contig_1", "start": 539227, "stop": 539625, "strand": "-", "frame": 2, "gene": null, "product": "COF family HAD hydrolase protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF26", "UniRef:UniRef90_C4XF26" ], "nt": "TTGAAATATAGTGATTTAGGTCAAAAAACTAATGTAACTAAAATTTTAACTTTTGGTAAGGATGAAATAGCTACTAAAAATTTTAAGAACTTTTTAGCTAAAAAATATCCTTCACTTGCATTTCATATAGTTTCAAAAGGTTACTCAATTGAAATCACACATAAAAATGCAACCAAAGGTAAAGGTGCAGCATTTGTTTGTGACTTATTAAAAATAGATCCTAAAAACGCTGCTCACATTGGTGATAGCGGAAATGATTTAAATGCTTTACCTGAGATAGGAACATTCATTGCTATGGAAAATGCAATGGATGAGGTAAAAGAAGTAGCACATTATATAGGGCCTTCTTTTGAAAATAGTGGAATAGCCAAGCTATTTAAAGATTTAAAAGAAATATAA", "aa": "MKYSDLGQKTNVTKILTFGKDEIATKNFKNFLAKKYPSLAFHIVSKGYSIEITHKNATKGKGAAFVCDLLKIDPKNAAHIGDSGNDLNALPEIGTFIAMENAMDEVKEVAHYIGPSFENSGIAKLFKDLKEI", "aa_hexdigest": "0c273b52d5694e192a0637ad9698282b", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 539227, "stop": 539925, "observations": { "mutation": [ 539646 ], "stop": [ 539646 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF26", "query_cov": 1.0, "subject_cov": 0.43137254901960786, "identity": 0.992, "score": 265.0, "evalue": 3.24e-87, "gene_start": 1, "gene_end": 132, "reference_start": 175, "reference_end": 306, "reference_sequence": "MNIFYQYIYKTALLVNDWKLGNYRYIFFVSYIKSLYIIKIMKKEIQAYFIDLDGTLLDLPEGEETLSSANVKAVVKLNKTKPVIISTGRSNSEFVLSLVKLLKCPYAICQNGALIVDKKNKVLARYEMEQNQVDEIVKLLKEEEMFFIFNGENKIYCNYESSAYMRPWTAKLEKLKYSDLGQKTNVTKILTFGKDEIATKNFKNFLAKKYPSLAFHIVSKGYSIEITHKNATKGKGAAFVCDLLKIDPKNAAHIGDSGNDLNALPEIGTFIAMENAMDEVKEVAHYIGPSFENSGIAKLFKDLKEI" }, "paralog": false, "identity": 0.9956896551724138, "score": 458.0, "evalue": 6.58e-166, "description": "Internal stop codon at 539646. Nonsense mutation around 539646.", "psc": { "uniref90_id": "UniRef90_C4XF26", "product": "COF family HAD hydrolase protein", "uniref50_id": "UniRef50_C4XF26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF26", "UniRef:UniRef50_C4XF26" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF26" ], "product": "COF family HAD hydrolase protein" } }, "id": "OAIKOHHJFB_989", "locus": "GCA000209735_00971" }, { "type": "cds", "sequence": "contig_1", "start": 539644, "stop": 540027, "strand": "-", "frame": 2, "gene": null, "product": "COF family HAD hydrolase protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KXX5" ], "nt": "ATGAAAAAAGAAATTCAAGCTTATTTTATAGATTTAGATGGAACATTATTAGACTTACCAGAAGGTGAAGAAACTCTTTCAAGTGCTAATGTTAAAGCTGTTGTTAAATTAAATAAAACTAAACCAGTTATTATTTCAACAGGTAGAAGTAATAGCGAATTTGTTTTATCCTTAGTTAAATTGTTAAAGTGCCCTTATGCCATTTGTCAAAATGGAGCTTTAATAGTTGATAAGAAAAATAAAGTTTTGGCTAGATATGAGATGGAACAAAATCAAGTTGATGAAATAGTTAAGCTTTTAAAAGAAGAAGAAATGTTTTTTATTTTTAATGGCGAAAATAAAATCTATTGTAACTATGAATCAAGTGCCTACATGCGCCCTTGA", "aa": "MKKEIQAYFIDLDGTLLDLPEGEETLSSANVKAVVKLNKTKPVIISTGRSNSEFVLSLVKLLKCPYAICQNGALIVDKKNKVLARYEMEQNQVDEIVKLLKEEEMFFIFNGENKIYCNYESSAYMRP", "aa_hexdigest": "e43ef937a803625d914152e38a7e467f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3P8KXX5", "query_cov": 0.8110236220472441, "subject_cov": 0.9716981132075472, "identity": 0.6990000000000001, "score": 153.0, "evalue": 3.94e-46, "valid": false, "product": "COF family HAD hydrolase protein", "uniref50_id": "UniRef50_A0A3P8KXX5" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KXX5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KXX5" ], "product": "COF family HAD hydrolase protein" }, "genes": [], "id": "OAIKOHHJFB_990", "locus": "GCA000209735_00972" }, { "type": "cds", "sequence": "contig_1", "start": 540463, "stop": 540876, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF27", "UniRef:UniRef90_C4XF27" ], "nt": "TTGTACAGTTTTGTAAAAAATAAATCTTTAATTAAAAACATTGAATCATTTGGTTCAACTTTGATTAAAGATTTGATCAAAAGAATCAACAAACAAAAATATATTAGAGTTCAAAAAGAACTATTTGGCAGTGGAAGCGAAGAATTAATAGTTCAAAATGATAAAGGCGTAATTATAATAGATTATAACCTTATTGTTTATGCTTTTTTTGAAGGCAACAGTTTATATGACTGTAAAAAGATAAGAGAATTTGTAAAAAAGAATTTTGATGAATCACTAACTCAGCTTGGCTGCGAAATATCAAAAGATCATTCTCTTTCATTAACAGCTGAAAATCTAACAATTGATGATTATAAAGATACTCCTTTTATAATTAATTTAAATATTGTTTTTGAATTTGGACCAGAATATTGA", "aa": "MYSFVKNKSLIKNIESFGSTLIKDLIKRINKQKYIRVQKELFGSGSEELIVQNDKGVIIIDYNLIVYAFFEGNSLYDCKKIREFVKKNFDESLTQLGCEISKDHSLSLTAENLTIDDYKDTPFIINLNIVFEFGPEY", "aa_hexdigest": "73a70c9b9263c566981e8471c4803850", "start_type": "TTG", "rbs_motif": "AGGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 540202, "stop": 540876, "observations": { "mutation": [ 540273, 540345, 540411, 540417, 540465 ], "stop": [ 540273, 540345, 540411, 540417, 540465 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF27", "query_cov": 1.0, "subject_cov": 0.6116071428571429, "identity": 1.0, "score": 273.0, "evalue": 8.54e-92, "gene_start": 1, "gene_end": 137, "reference_start": 1, "reference_end": 137, "reference_sequence": "MYSFVKNKSLIKNIESFGSTLIKDLIKRINKQKYIRVQKELFGSGSEELIVQNDKGVIIIDYNLIVYAFFEGNSLYDCKKIREFVKKNFDESLTQLGCEISKDHSLSLTAENLTIDDYKDTPFIINLNIVFEFGPEYWIQLAHFKKSDAKKERWFWYNVPNTNSLMKRVEECKECDLWKDVQNIYLEKMNDFSKIFNRNADWFYCYAAAITEVENKNSDYDGED" }, "paralog": false, "identity": 0.9732142857142857, "score": 434.0, "evalue": 2.28e-157, "description": "Internal stop codon at 540273, 540345, 540411, 540417, 540465. Nonsense mutation around 540273, 540345, 540411, 540417, 540465.", "psc": { "uniref90_id": "UniRef90_C4XF27", "uniref50_id": "UniRef50_C4XF27", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF27", "UniRef:UniRef50_C4XF27" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF27", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF27" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 15895.071899999988, "isoelectric_point": 5.480665779113769 }, "id": "OAIKOHHJFB_991", "locus": "GCA000209735_00973" }, { "type": "cds", "sequence": "contig_1", "start": 541180, "stop": 541521, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATATTTTAGTATTCAAAAATAAATATGTTGATTATCAAGATACTGAAAATTACAATTTTGAAACTGTTTCAAATAATAGTTTCCAAAAGTTGGCAAAAGAAAAATATAATGAATTTTTAGCTAAATTTCAAGATTTAAAAATTAATAAAATTTTTGTTAAAGATCTTCAACCTTTTTATTCAAGTAATGACAGTGAAACTATAAAGCTTTTAGAATTTTATATTTATCTTTATAAAGCACTTTATTGTACTTTATCAAACTTTATATCATTAGCGTATAGTAAAGAAGATGCTTTAAATAAATGTATCAACATTAAAGATTATTGTCACGAGTATTGA", "aa": "MDILVFKNKYVDYQDTENYNFETVSNNSFQKLAKEKYNEFLAKFQDLKINKIFVKDLQPFYSSNDSETIKLLEFYIYLYKALYCTLSNFISLAYSKEDALNKCINIKDYCHEY", "aa_hexdigest": "47f00f766684777b481db34dbd78112b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13607.305399999987, "isoelectric_point": 5.3758546829223635 }, "id": "OAIKOHHJFB_992", "locus": "GCA000209735_00974" }, { "type": "cds", "sequence": "contig_1", "start": 541801, "stop": 542076, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAGATATAACAATTACACAAATGCAATTTAATGAATATTTATATGATTATAGTCAAGATCAAGAAAAGAAAATTCGTTTAGATGATTGGACTGCTTTTGATGAATATTATTATGAATTATTAAAAAAATATATCAAAGAGAAAGTTACTAATACTAATGAGAAAATAAGAATTTATTTCTTGCCTCGTTATGATAATAAATATTATGCTTTTAGACTTAAATATGAAAGTGAAAAAGAAGCTGAAAAAGAATTAGGCAAAGAAGAATGA", "aa": "MKLDITITQMQFNEYLYDYSQDQEKKIRLDDWTAFDEYYYELLKKYIKEKVTNTNEKIRIYFLPRYDNKYYAFRLKYESEKEAEKELGKEE", "aa_hexdigest": "d4bf6510253b5d325f7b78432f4ea127", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11487.806199999992, "isoelectric_point": 5.140995979309081 }, "id": "OAIKOHHJFB_993", "locus": "GCA000209735_00975" }, { "type": "cds", "sequence": "contig_1", "start": 542256, "stop": 542477, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XC57", "UniRef:UniRef90_A0AB32XC57" ], "nt": "ATGTTCAAAAATGTTAATATCGATTTTCTCCCTTATTACATTGTTGATGGCCAAGGTTATAAGAGCTTAATTAGAAAATATCAAGTAATAACTTCTTCTAAGAATGCAAAATTTGAAATAGTCTATGGTTACTTATTACTAGTAGCAAGCATTAACTTAATTTTATTAGATTATTTAAATCAAAATAGTATTAATAATTCTGCTTTTGAATATGAAGTTTAG", "aa": "MFKNVNIDFLPYYIVDGQGYKSLIRKYQVITSSKNAKFEIVYGYLLLVASINLILLDYLNQNSINNSAFEYEV", "aa_hexdigest": "ce759fb92ec6a27806a2e278e9e0d2d9", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 542256, "stop": 542600, "observations": { "mutation": [ 542498 ], "stop": [ 542498 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XC57", "query_cov": 1.0, "subject_cov": 0.6403508771929824, "identity": 1.0, "score": 143.0, "evalue": 7.36e-43, "gene_start": 1, "gene_end": 73, "reference_start": 42, "reference_end": 114, "reference_sequence": "MNNEFTNFIFKEKIITNLYDNDNKLTKELKNKNIWDILNYKMFKNVNIDFLPYYIVDGQGYKSLIRKYQVITSSKNAKFEIVYGYLLLVASINLILLDYLNQNSINNSAFEYEV" }, "paralog": false, "identity": 0.9824561403508771, "score": 224.0, "evalue": 1.75e-77, "description": "Internal stop codon at 542498. Nonsense mutation around 542498.", "psc": { "uniref90_id": "UniRef90_A0AB32XC57", "uniref50_id": "UniRef50_A0AB32XC57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XC57", "UniRef:UniRef50_A0AB32XC57" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB32XC57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XC57" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8493.687599999996, "isoelectric_point": 6.058888816833496 }, "id": "OAIKOHHJFB_994", "locus": "GCA000209735_00976" }, { "type": "cds", "sequence": "contig_1", "start": 542973, "stop": 543110, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCTGGATTAACAAGAGAAATTTATACTAGAAAAGGTAAAGTTAATAGTAGTGGAGCTTATCAAGGACCTAAATATTTCCAATTTAATGGTAAAAGGCTTACTGAATTGCCAATAATTGAAGAATGCCAATCTTAA", "aa": "MSGLTREIYTRKGKVNSSGAYQGPKYFQFNGKRLTELPIIEECQS", "aa_hexdigest": "c4eab319b03caa553623bfe6a41946fa", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5126.778799999998, "isoelectric_point": 9.358891487121582 }, "id": "OAIKOHHJFB_995", "locus": "GCA000209735_00977" }, { "type": "cds", "sequence": "contig_1", "start": 543114, "stop": 543608, "strand": "-", "frame": 3, "gene": null, "product": "Bacteriophage T5 Orf172 DNA-binding domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF30", "UniRef:UniRef90_C4XF30" ], "nt": "ATGCCAGAAAAAGGATATGTTTATGTTTTAACTAACCCAAGTTTTAGAGATGATTGGGTTAAAATTGGTAAAAGCAGTAGAATGCCTGAAGTTAGAGGTCGTGAATTATATAATACAGCTGTTCCACTTCCTTATGAAATCTATGCCACTTTACAAACTGAAAAATATAATGAAGTTGAAAAAATGATTCACAAAAGTATTGATCGAATTTCAAAATTAAGAATAAACAAAAATAGAGAATTTTTTAATATACCACCTGAAAAAGCTTATGACATTTTACTTGATATTAAGGAATTATTAGGAAATGAAGCTAAATTAGAATTAATGGGTGATAATGTCGAAGTACATACTAAAGAAAAAACAAAAAGAGGTGAACCATTTGACTTTTATAGTAGAGGTTTAAAAGATGGAGATGTTGTTTCATTCATAGAAAATTCAAACATAAAAGCAATTGTTGAATCAAATAAAACTGTATATTTTGAAAATCAAAGTTGA", "aa": "MPEKGYVYVLTNPSFRDDWVKIGKSSRMPEVRGRELYNTAVPLPYEIYATLQTEKYNEVEKMIHKSIDRISKLRINKNREFFNIPPEKAYDILLDIKELLGNEAKLELMGDNVEVHTKEKTKRGEPFDFYSRGLKDGDVVSFIENSNIKAIVESNKTVYFENQS", "aa_hexdigest": "af3ece7a340f895bec58c09b9b2a5269", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 542973, "stop": 543608, "observations": { "mutation": [ 543116 ], "stop": [ 543116 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF30", "query_cov": 1.0, "subject_cov": 0.7772511848341233, "identity": 1.0, "score": 328.0, "evalue": 5.47e-113, "gene_start": 1, "gene_end": 164, "reference_start": 1, "reference_end": 164, "reference_sequence": "MPEKGYVYVLTNPSFRDDWVKIGKSSRMPEVRGRELYNTAVPLPYEIYATLQTEKYNEVEKMIHKSIDRISKLRINKNREFFNIPPEKAYDILLDIKELLGNEAKLELMGDNVEVHTKEKTKRGEPFDFYSRGLKDGDVVSFIENSNIKAIVESNKTVYFENQSWSLSGLTREIYTRKGKVNSSGAYQGPKYFQFNGKRLTELPIIEECQS" }, "paralog": false, "identity": 0.995260663507109, "score": 422.0, "evalue": 1.03e-152, "description": "Internal stop codon at 543116. Nonsense mutation around 543116.", "psc": { "uniref90_id": "UniRef90_C4XF30", "product": "Bacteriophage T5 Orf172 DNA-binding domain-containing protein", "uniref50_id": "UniRef50_C4XF30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF30", "UniRef:UniRef50_C4XF30" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF30" ], "product": "Bacteriophage T5 Orf172 DNA-binding domain-containing protein" } }, "id": "OAIKOHHJFB_996", "locus": "GCA000209735_00978" }, { "type": "cds", "sequence": "contig_1", "start": 544206, "stop": 544355, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATTTTTTATAAATCAAGTATTTATCAAACATTAGTTAAAAATAATTCAAGAAGTGCGCAAGCCGGATTTAATAAAGACGATTTAAAAAATCTTTTTATGCCAATTCCTTCTTTAAATGAATCGTCAAGAATTGTAAGTAAATGA", "aa": "MFIFYKSSIYQTLVKNNSRSAQAGFNKDDLKNLFMPIPSLNESSRIVSK", "aa_hexdigest": "7a5dbe482dff17f5a06cda5d26a867b0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5629.4245999999985, "isoelectric_point": 9.871673011779784 }, "id": "OAIKOHHJFB_997", "locus": "GCA000209735_00979" }, { "type": "cds", "sequence": "contig_1", "start": 544452, "stop": 545129, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_A0AB32XCC0", "UniRef:UniRef90_A0AB32XCC0", "PFAM:PF01420.25" ], "nt": "TTGCAATATGCAATTCAGGGTAAATTAGTTAAACAAGATCCCAATGACGAACCAGCTTCTAAATTATTAGAAGCTATACAAATTGAAAAAAATGAGCTAATTAAAGAAGGAAAAATAAAGAAAGATAAACAAGAATCTTTCATTTTTCAAGGTGAAGATAAAAATTATTATGAGAAGATTGGTTCGAAAGTAATAAATATTACAAATGAAATACCTTTCGAAATACCAAAAAAATGGGCTTGGGTTAGACAAAAAAACATTTTAAAATTGACAAAAAATGAAGCTTCTAAAAATGGAAATTACCCTTATCTAGAAGCAAAGGTACTAAGAAAAATTATTAAACCGAAAATTATTAATAATGGGGTTTTAATAAATAAAGGAGATATTGTTATTTTGGTTGATGGTGAGAATTCAGGTGAAACATTTGTTCTAGACCAAACTGGTTATATGGGTAGTACATTTAAACTATTAAAAATAAATAACAAAATAGATCAGGAATATGTTTTAATGTTATTAAAATTTTATAAAGAATTATTCAAGAAAAATAAAAAAGGAGCTGCAATACCACATTTAAATATAGACATTTTCAATAATTTACTTTTGGCTATTCCAAACATAAAAGAACAAAAAGAAATCATTTTAAAACTTAAAAAGATCGATAATTTTATTAGTTATTAA", "aa": "MQYAIQGKLVKQDPNDEPASKLLEAIQIEKNELIKEGKIKKDKQESFIFQGEDKNYYEKIGSKVINITNEIPFEIPKKWAWVRQKNILKLTKNEASKNGNYPYLEAKVLRKIIKPKIINNGVLINKGDIVILVDGENSGETFVLDQTGYMGSTFKLLKINNKIDQEYVLMLLKFYKELFKKNKKGAAIPHLNIDIFNNLLLAIPNIKEQKEIILKLKKIDNFISY", "aa_hexdigest": "9ad2b836683eb0c06b55bf8f83e450fc", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB32XCC0", "query_cov": 1.0, "subject_cov": 0.9, "identity": 0.987, "score": 432.0, "evalue": 1.85e-152, "valid": true, "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_A0AB32XCC0", "kegg_orthology_id": "K01154" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XCC0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCC0" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 156, "aa_cov": 0.6533333333333333, "hmm_cov": 0.9935897435897436, "evalue": 9.903038661716084e-16, "score": 54.481224060058594, "start": 77, "stop": 223 } ], "seq_stats": { "molecular_weight": 26027.235200000032, "isoelectric_point": 9.443602943420409 }, "id": "OAIKOHHJFB_998", "locus": "GCA000209735_00980" }, { "type": "cds", "sequence": "contig_1", "start": 545091, "stop": 545480, "strand": "-", "frame": 3, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E52004D" ], "nt": "ATGAAAGATAAACAAATTATTGAAGATTCTAAGAAAAAAATATCTCTCCAAGCAAAAAAAGAAATATTTAATAATCAAATGTCCAAAAAAGGAACTTTATTATTGAGTTTTAAATTAACAATAGGTAAAACATCAATAATTAATCAAGATTCAGTTCATAATGAAGCAATTGTTTCAATAAATTTTTACAAAGATAATAATATAACAAAAATGTTTCTATTAATATTTTTAGGATTATTGATCAATAATTGTGAAAAAATTAATGCTATCAAAGGAAAAACATTAAATAAGGAAAAATTGCAAAAAATGTTAATTCCTATACCCCCTATCAAAAATCAAAATAATATTTTATTAATAACTAATAAAATTATCGATCTTTTTAAGTTTTAA", "aa": "MKDKQIIEDSKKKISLQAKKEIFNNQMSKKGTLLLSFKLTIGKTSIINQDSVHNEAIVSINFYKDNNITKMFLLIFLGLLINNCEKINAIKGKTLNKEKLQKMLIPIPPIKNQNNILLITNKIIDLFKF", "aa_hexdigest": "8b7d4485e7035ed4d65a5483751366dd", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001E52004D", "query_cov": 0.9534883720930233, "subject_cov": 0.8424657534246576, "identity": 0.504, "score": 103.0, "evalue": 7.61e-26, "valid": false, "product": "restriction endonuclease subunit S", "uniref50_id": "UniRef50_UPI001E52004D" }, "pscc": { "uniref50_id": "UniRef50_UPI001E52004D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E52004D" ], "product": "restriction endonuclease subunit S" }, "genes": [], "id": "OAIKOHHJFB_999", "locus": "GCA000209735_00981" }, { "type": "cds", "sequence": "contig_1", "start": 545592, "stop": 545987, "strand": "-", "frame": 3, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A83F32A" ], "nt": "TTGCTAAATATTTTAATTGCTATTCCGCCAATAAATGAACAAGAAAAAATCATTAACAAATTAATATCATTAGATAAATTTATGAAGAAATATTTAGAAAAAGAAAATCAATTATTCAAACTAGATTCTGAAATTAAAGACAAATTACAAAAATCTATCTTACAATATGCAATTCAAGGAAAATTAGTTAAACAAGATCCTAATGATGAACCAGCATCAAAATTATTAGAAGCTATACAAATTGAAAAAAACAAACTAATTAAAGAAGGAAAAATAAAGAAAGATAAACATGAATCTTTCATTTTTCAAGGTGAAGATAAAAATTATTATGAGAAGATTGGTTCGAAAGTAATAAATATTACAAATGAAATACCTTTCGAAATACCAATAAATTGA", "aa": "MLNILIAIPPINEQEKIINKLISLDKFMKKYLEKENQLFKLDSEIKDKLQKSILQYAIQGKLVKQDPNDEPASKLLEAIQIEKNKLIKEGKIKKDKHESFIFQGEDKNYYEKIGSKVINITNEIPFEIPIN", "aa_hexdigest": "7c1938e22b4beccb1cd65827274a58a5", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000A5E1817", "query_cov": 0.8320610687022901, "subject_cov": 0.8074074074074075, "identity": 0.541, "score": 99.0, "evalue": 3.93e-24, "valid": false, "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_UPI000A83F32A", "kegg_orthology_id": "K01154" }, "pscc": { "uniref50_id": "UniRef50_UPI000A83F32A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A83F32A" ], "product": "restriction endonuclease subunit S" }, "genes": [], "id": "OAIKOHHJFB_1000", "locus": "GCA000209735_00982" }, { "type": "gap", "sequence": "contig_1", "start": 546105, "stop": 546204, "strand": ".", "length": 100, "id": "OAIKOHHJFB_1803" }, { "type": "cds", "sequence": "contig_1", "start": 546501, "stop": 546812, "strand": "-", "frame": 3, "gene": null, "product": "Restriction endonuclease subunit S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_V6Z525" ], "nt": "GTGTTATATAAAAAAACAGAAAATCAACTATTTAAACTTGATTCCGAAATTAAAGATAAATTGCAAAAATCTATTTTGCAATATGCAATTCAGGGAAAATTGGTTAAACAAGATCCTAATGATGAACCAGCTTCTAAGCTTTTAGAAGCTATACAAATTGAAAAAAATAAGCTAATTAAAGAAGGAAAAATAAAGAAAGATAAACAAGAATCTTTAATTTTTCAAGGTGAAGATAAAAATTATTATGAGAAGATTGGCTCAAAAGTTATAAATATAACAAACGAAATACCTTTTGAAATACCAAAAAATTGA", "aa": "MLYKKTENQLFKLDSEIKDKLQKSILQYAIQGKLVKQDPNDEPASKLLEAIQIEKNKLIKEGKIKKDKQESLIFQGEDKNYYEKIGSKVINITNEIPFEIPKN", "aa_hexdigest": "e0757cd0c572be40ae549107e4c1f9d0", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A172W5V5", "query_cov": 0.8252427184466019, "subject_cov": 0.8673469387755102, "identity": 0.541, "score": 78.2, "evalue": 7.84e-17, "valid": false, "product": "HsdS-A9", "uniref50_id": "UniRef50_V6Z525" }, "pscc": { "uniref50_id": "UniRef50_V6Z525", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_V6Z525" ], "product": "Restriction endonuclease subunit S" }, "genes": [], "id": "OAIKOHHJFB_1001", "locus": "GCA000209735_00983" }, { "type": "gap", "sequence": "contig_1", "start": 547331, "stop": 547430, "strand": ".", "length": 100, "id": "OAIKOHHJFB_1804" }, { "type": "cds", "sequence": "contig_1", "start": 548751, "stop": 548900, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAAAAAAGGCTTTAAAGCTGGCAAGAGCAAGAAACAAGTTGCAATTAAGAAATTCAAAGAAATACAAATCTATAAAAAAGACAGAATTAAGGCTAAGCCGGTTGTTCTTAGCGATGAAGAAATTAATAAATTATTTGCAAAATAA", "aa": "MNKKGFKAGKSKKQVAIKKFKEIQIYKKDRIKAKPVVLSDEEINKLFAK", "aa_hexdigest": "d9f0168a356d3bfa98a930622ed41f2c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5693.836700000001, "isoelectric_point": 10.22006015777588 }, "id": "OAIKOHHJFB_1002", "locus": "GCA000209735_00984" }, { "type": "gap", "sequence": "contig_1", "start": 549252, "stop": 549252, "strand": ".", "length": 1, "id": "OAIKOHHJFB_1805" }, { "type": "gap", "sequence": "contig_1", "start": 549291, "stop": 549390, "strand": ".", "length": 100, "id": "OAIKOHHJFB_1806" }, { "type": "cds", "sequence": "contig_1", "start": 549687, "stop": 549887, "strand": "+", "frame": 3, "gene": null, "product": "Archaeal holliday junction resolvase (hjc)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF36", "UniRef:UniRef90_C4XF36" ], "nt": "TTGTCTATTCTAAAAGCTAAAGGTTACCAAGCTAAGAGAACAAATCTTAAAATGGATGGCATATATAAAATTGATATAGTGGCAACAAAAGGAAATGAGAAGATTTATATTCAAGTTAAGTTAAACAAACCAAAAGCTTTAGAATTGAAGAATTTGCATTCATTCTTAAGATCTAAGGCCTTTCAAGGACTAATTGCATGA", "aa": "MSILKAKGYQAKRTNLKMDGIYKIDIVATKGNEKIYIQVKLNKPKALELKNLHSFLRSKAFQGLIA", "aa_hexdigest": "0f7b71e56878e69c8b68c51f46a7ce5f", "start_type": "TTG", "rbs_motif": "AGxAG", "genes": [], "truncated": "both", "pseudogene": { "start": 549426, "stop": 549923, "observations": { "mutation": [ 549540, 549885, 549903 ], "stop": [ 549540, 549885, 549903 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF36", "query_cov": 1.0, "subject_cov": 0.4, "identity": 0.985, "score": 125.0, "evalue": 1.78e-35, "gene_start": 1, "gene_end": 66, "reference_start": 88, "reference_end": 153, "reference_sequence": "MYENFSRYNLTMKMPNYKDLNFNIKRELNKLKPKSFEEWKEAKSKVFCLALGKYLTKINSHQPDIFKNYYQVLFYHSFINYKREEELLSILKAKGYQAKRTNLKMDGIYKIDIVATKGNEKIYIQVKLNKPKALELKNLHSFLRSKAFQGLIAWKESTEWKFKEI" }, "paralog": false, "identity": 0.9757575757575757, "score": 319.0, "evalue": 3.04e-114, "description": "Internal stop codon at 549540, 549885, 549903. Nonsense mutation around 549540, 549885, 549903.", "psc": { "uniref90_id": "UniRef90_C4XF36", "product": "Archaeal holliday junction resolvase (hjc)", "uniref50_id": "UniRef50_C4XF36", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF36", "UniRef:UniRef50_C4XF36" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF36", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF36" ] } }, "id": "OAIKOHHJFB_1003", "locus": "GCA000209735_00985" }, { "type": "cds", "sequence": "contig_1", "start": 550271, "stop": 550396, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGAAAGTTGAAAGAATTTATGATATGGTTGGCAAATTCTTAGGACGTGAAAACAAGAAATTTGTTTTTAATGATCTTAATTTGAATTTAAAAAGCAAACGCTGTATACCTTCTATAGACTGA", "aa": "MMKVERIYDMVGKFLGRENKKFVFNDLNLNLKSKRCIPSID", "aa_hexdigest": "2baba0b95ee0a5e4699a863967c3e6b0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4891.8009, "isoelectric_point": 9.65273838043213 }, "id": "OAIKOHHJFB_1004", "locus": "GCA000209735_00986" }, { "type": "cds", "sequence": "contig_1", "start": 550508, "stop": 550645, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTATTTCACAATTAGATGTTGAAAGTATGAATGATTTAAGAATCAATAAAAACTTCAATACATATAATGGCGCCTTGTATGAAAATGCAATAGCTAATAGTTTATACAAACAGGACTTTAAATTATTTTATTGA", "aa": "MLISQLDVESMNDLRINKNFNTYNGALYENAIANSLYKQDFKLFY", "aa_hexdigest": "e7e701ce5ad9e6acd238915b2cca13f5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5323.961100000001, "isoelectric_point": 4.8610639572143555 }, "id": "OAIKOHHJFB_1005", "locus": "GCA000209735_00987" }, { "type": "cds", "sequence": "contig_1", "start": 551277, "stop": 551504, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTCTTAAGAAGACCTTTTGGTGTAACTCCTGCAATCTTAGCAATAGCAGATCACATTACAAAGCTAAAAGCGGTATGCATGAAATGTAAATCTAGTGCAGGTTTTAGTTTTAGAAAAACAAACGAACAATCATTAAATTCGCTTGGTGATGATACTTATGAAGCTCGTTGTAGAGTCTGCCACATCAAAGGTGAACTAAAGAAAAAAGAAGACAAAAAATAA", "aa": "MDFLRRPFGVTPAILAIADHITKLKAVCMKCKSSAGFSFRKTNEQSLNSLGDDTYEARCRVCHIKGELKKKEDKK", "aa_hexdigest": "679e689a618fc70ef86a201cca496f56", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8475.851199999996, "isoelectric_point": 9.509747505187988 }, "id": "OAIKOHHJFB_1006", "locus": "GCA000209735_00988" }, { "type": "cds", "sequence": "contig_1", "start": 551622, "stop": 551855, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATTTAAAACAAAGTGATGGGATTTTAGAAGTTATAACTGGCCCAATGTTCTCAGGTAAAACAGAAGAGCTTATTAAAAGAATTAAAATTTTAGAAATAGCAGATATTAAAACTTTAGTTCTTAAACCTAAATTTGATACTCGCTTTTCTGAAACAGAAATAGTAAGTAGAACAGGTGCAAAAGTTATAGCTAAAAATATTAAAAAATCAAGTGATATAATGAAATTTTGA", "aa": "MYLKQSDGILEVITGPMFSGKTEELIKRIKILEIADIKTLVLKPKFDTRFSETEIVSRTGAKVIAKNIKKSSDIMKF", "aa_hexdigest": "200ea860849e074e752178a5ca9bc592", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8732.321499999995, "isoelectric_point": 9.646098136901855 }, "id": "OAIKOHHJFB_1007", "locus": "GCA000209735_00989" }, { "type": "cds", "sequence": "contig_1", "start": 551928, "stop": 552155, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00883.26" ], "nt": "ATGCCATTTGATGAAGCATTCTCAAAAGGAATCAAAGCTTCTAAAGTTGCTGATCTTAAAAACACAGACTTAAGCGGTTATGGTGGATCAAGCTCAGCTGCTATGTTCTTAAAAGAATTTACAGAAGGCGTTGAATACATCCACTGTGATGTCGCAGGTACAGCAGACATTAATGAAGTGCCACAAGGTGTTTTAGTCAAAACACTTACAGAGCTTGCTTTAATTTAA", "aa": "MPFDEAFSKGIKASKVADLKNTDLSGYGGSSSAAMFLKEFTEGVEYIHCDVAGTADINEVPQGVLVKTLTELALI", "aa_hexdigest": "2494b773a88588688a397ca7310615fb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00883.26", "name": "Peptidase_M17", "length": 77, "aa_cov": 0.92, "hmm_cov": 0.974025974025974, "evalue": 1.836558068197406e-14, "score": 49.7672004699707, "start": 1, "stop": 69 } ], "seq_stats": { "molecular_weight": 7940.935899999997, "isoelectric_point": 4.534353446960449 }, "id": "OAIKOHHJFB_1008", "locus": "GCA000209735_00990" }, { "type": "cds", "sequence": "contig_1", "start": 552225, "stop": 552395, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00883.26" ], "nt": "ATGAATGGTAAAAGCGTTGAAATCAACAACACAGATGCTGAGGGTCGTTTAGTTATGGCTGATGGTTTAGTTTATGGAGCAACCAAACTTCATGCAACACGTTTAATTGACGTTGCTACATTAACTGGTGCTATTCTTATTGCTTTAGGTCAAACCTACACAGGTATTTGA", "aa": "MNGKSVEINNTDAEGRLVMADGLVYGATKLHATRLIDVATLTGAILIALGQTYTGI", "aa_hexdigest": "6db99d060ca59c84b65830bf83bc4fd0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00883.26", "name": "Peptidase_M17", "length": 56, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 9.248403010825994e-24, "score": 80.30740356445312, "start": 1, "stop": 56 } ], "seq_stats": { "molecular_weight": 5832.660399999999, "isoelectric_point": 5.498172187805174 }, "id": "OAIKOHHJFB_1009", "locus": "GCA000209735_00991" }, { "type": "cds", "sequence": "contig_1", "start": 552402, "stop": 553301, "strand": "-", "frame": 3, "gene": null, "product": "Cytosol aminopeptidase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47707", "UniRef:UniRef90_C4XF40" ], "nt": "ATGTCACTTTTTACAAAATTAGTAAATAAAGCTACTAAAGATGTTTTATTGCAAGCAACTTTTAAAGATGCTAAAAAGATTGAATATTTAATCGAAAAAAACAACGAAATTACAGAATATCTTAGTGAAAACAAAGCTTATGTTTACTTAGGAGAAGAAAGCAAGTTAGATTTTAGAACTCTTGAAAGATTTTTTAGATCTTTAAGTGAAAATAGCGCACGTAACTATTGTGTTGATTTAGCTTCATTTGCAACAAAAAATGTTTCAGTTGCTGATGTTGTTGAAGCTTTTACAAGAGCAGTTTACTTTGTTAAAGCTTCTTTATACAATGAAAAAGCTAAAGATAAAGATGCTAAAGAATTTACTTTAGTTCCTTATGTAGAAAAAGTAACTAAAGAAGTTGAAGAAAGATTAGAAAAAACATTAATTTTAGCTGAAGCTACAAATTATGCTCGTAACTTACAAGTTACACCACCAAACATTTGCAACTCAGAATATTTAGCAGACCAAATTGTTAAAGATTTCAAACAATACAAAAACTTAAAAGTTACTGTTTTAAATAAAAAACAAATTCAAGACCTTAAAATGGGATTATTACTTTCAGTTAACAGAGGGTCAGTTTATGAACCACGTGTAGTTGTTATCGAATATGCTGGTGATCCTTCAAGTAAAGAAAAAACAGTTTTAGTCGGTAAAGGTATTACTTTTGACTCAGGTGGTTACTCACTTAAGAGTCCAAGAAACATGTTAGGTATGAAATTCGACATGTCAGGTAGTGTTATTGTTGCTTCAACATTAAAAGCTATCGCACAACTTAAACCTAAGAAAAATGTTTCAGCAATTATGTGTATTACAGACAACAGAGTTAATGGTGATGCTTCATTACCTGACTCAGTTTGA", "aa": "MSLFTKLVNKATKDVLLQATFKDAKKIEYLIEKNNEITEYLSENKAYVYLGEESKLDFRTLERFFRSLSENSARNYCVDLASFATKNVSVADVVEAFTRAVYFVKASLYNEKAKDKDAKEFTLVPYVEKVTKEVEERLEKTLILAEATNYARNLQVTPPNICNSEYLADQIVKDFKQYKNLKVTVLNKKQIQDLKMGLLLSVNRGSVYEPRVVVIEYAGDPSSKEKTVLVGKGITFDSGGYSLKSPRNMLGMKFDMSGSVIVASTLKAIAQLKPKKNVSAIMCITDNRVNGDASLPDSV", "aa_hexdigest": "4c7d84cb938ae47eaed40a3780dfca4d", "start_type": "ATG", "rbs_motif": "AGGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 552099, "stop": 553331, "observations": { "mutation": [ 552161, 552206, 552227, 552404 ], "stop": [ 552161, 552206, 552227, 552404 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF40", "query_cov": 1.0, "subject_cov": 0.6402569593147751, "identity": 1.0, "score": 566.0, "evalue": 6.34e-201, "gene_start": 1, "gene_end": 299, "reference_start": 11, "reference_end": 309, "reference_sequence": "MIKLNNRRLIMSLFTKLVNKATKDVLLQATFKDAKKIEYLIEKNNEITEYLSENKAYVYLGEESKLDFRTLERFFRSLSENSARNYCVDLASFATKNVSVADVVEAFTRAVYFVKASLYNEKAKDKDAKEFTLVPYVEKVTKEVEERLEKTLILAEATNYARNLQVTPPNICNSEYLADQIVKDFKQYKNLKVTVLNKKQIQDLKMGLLLSVNRGSVYEPRVVVIEYAGDPSSKEKTVLVGKGITFDSGGYSLKSPRNMLGMKFDMSGSVIVASTLKAIAQLKPKKNVSAIMCITDNRVNGDASLPDSVWKAMNGKSVEINNTDAEGRLVMADGLVYGATKLHATRLIDVATLTGAILIALGQTYTGIWATSEKAWEDISKAADNAHELVWRMPFDEAFSKGIKASKVADLKNTDLSGYGGSSSAAMFLKEFTEGVEYIHCDVAGTADINEVPQGVLVKTLTELALI" }, "paralog": false, "identity": 0.9878048780487805, "score": 772.0, "evalue": 1.45e-284, "description": "Internal stop codon at 552161, 552206, 552227, 552404. Nonsense mutation around 552161, 552206, 552227, 552404.", "psc": { "uniref90_id": "UniRef90_C4XF40", "product": "Cytosol aminopeptidase domain-containing protein", "uniref50_id": "UniRef50_P47707", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF40", "UniRef:UniRef50_P47707" ] }, "pscc": { "uniref50_id": "UniRef50_P47707", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47707" ], "product": "Probable cytosol aminopeptidase" } }, "id": "OAIKOHHJFB_1010", "locus": "GCA000209735_00992" }, { "type": "cds", "sequence": "contig_1", "start": 553510, "stop": 553890, "strand": "-", "frame": 2, "gene": "phoU", "product": "PhoU domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF41", "UniRef:UniRef50_UPI0038737223", "UniRef:UniRef90_C4XF41" ], "nt": "ATGGCCAGATTCTTCTATCAAAACAAAAAAATTCCTCAAGAGGTTGTTAATGCTGCTTTAGCTATGAATATAGAATTAAGCAAAATTTTTAAAGAATATAGTAGCCGTCTTCAAGATGAAAAAAGTACTAAAGAATTCTTCAAAGAATTCCAAAAACAAAAAGTAGAATACAAACAATTCTGTGATAAAGAGATCAAAAAAATGGCTCGTAAATTAACCAAAAATGATGAGTATTCTAACGAAATAATTATTAAGTTTTCTTTTGAATTGCACTCATATTATCGTGTAGTTGAAAGACTAGAAAATATCATTGAAAATTTGATTTTCATTCATGATACAGATTTCTTTAGAAAGAAATTGGAAAATGAAGAAATGTTTTAA", "aa": "MARFFYQNKKIPQEVVNAALAMNIELSKIFKEYSSRLQDEKSTKEFFKEFQKQKVEYKQFCDKEIKKMARKLTKNDEYSNEIIIKFSFELHSYYRVVERLENIIENLIFIHDTDFFRKKLENEEMF", "aa_hexdigest": "db0ebe688e8bd3145b04cc565b6aeec5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0038737223", "query_cov": 1.0, "subject_cov": 1.024390243902439, "identity": 0.635, "score": 158.0, "evalue": 9.57e-48, "valid": false, "uniref50_id": "UniRef50_UPI0038737223" }, "pscc": { "uniref50_id": "UniRef50_UPI0038737223", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0038737223" ] }, "genes": [ "phoU" ], "truncated": "3-prime", "pseudogene": { "start": 553510, "stop": 554190, "observations": { "mutation": [ 553953, 553995 ], "stop": [ 553953, 553995 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF41", "query_cov": 1.0, "subject_cov": 0.5384615384615384, "identity": 1.0, "score": 242.0, "evalue": 2.29e-79, "gene_start": 1, "gene_end": 126, "reference_start": 109, "reference_end": 234, "reference_sequence": "MMPINYTILKKSEDNLRKMFFDYLDQAYQMHLKLTELISIYQNKIDIEGLFLEINNREKQSDLTQVNTIEELLWDISKDQPLLSHLRWYICVLTSSGDIERICDYLYFMARFFYQNKKIPQEVVNAALAMNIELSKIFKEYSSRLQDEKSTKEFFKEFQKQKVEYKQFCDKEIKKMARKLTKNDEYSNEIIIKFSFELHSYYRVVERLENIIENLIFIHDTDFFRKKLENEEMF" }, "paralog": false, "identity": 0.9911504424778761, "score": 442.0, "evalue": 6.76e-161, "description": "Internal stop codon at 553953, 553995. Nonsense mutation around 553953, 553995.", "psc": { "uniref90_id": "UniRef90_C4XF41", "product": "PhoU domain-containing protein", "uniref50_id": "UniRef50_C4XF41", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF41", "UniRef:UniRef50_C4XF41" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF41", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF41" ], "product": "PhoU domain-containing protein" } }, "id": "OAIKOHHJFB_1011", "locus": "GCA000209735_00993" }, { "type": "cds", "sequence": "contig_1", "start": 553993, "stop": 554211, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAATAAATTACACTATATTAAAGAAGAGCGAAGATAACTTAAGAAAAATGTTTTTTGATTACTTAGACCAAGCATATCAAATGCATCTTAAATTAACTGAACTTATTTCAATATATCAAAACAAAATTGATATTGAAGGCTTATTTTTAGAAATCAATAATCGTGAAAAACAAAGCGACTTAACTCAAGTAAATACCATTGAAGAATTGCTTTGA", "aa": "MPINYTILKKSEDNLRKMFFDYLDQAYQMHLKLTELISIYQNKIDIEGLFLEINNREKQSDLTQVNTIEELL", "aa_hexdigest": "60f59d4d414ef04a68b2c46a124400e2", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8668.875899999992, "isoelectric_point": 4.781091499328612 }, "id": "OAIKOHHJFB_1012", "locus": "GCA000209735_00994" }, { "type": "cds", "sequence": "contig_1", "start": 554216, "stop": 554827, "strand": "-", "frame": 1, "gene": "pstB", "product": "Phosphate import ATP-binding protein PstB 3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0A2V9" ], "nt": "TTGAATCGAATTCATGGGCCACAAGCTGGAAATTATCTAGAAGGTAAAGTTGAATACTTAAATCAAGATCTCTTTAGCAAAAAATTTATTAATGATATTGAATTAAGAAAGCAAGTCGGAATGATATTTCAACAGCCAAGTGTTTTCCCTCTTTCTATTTATGATAATGTTGCTATTGGACTAAGAAATATTGGAATTAGAGATAAAAAAATATTAGACCAAAAAGTGCAAGAAGCTTTAGAAAGAGCTGCACTTTATGATGAAGTTAAAGATAAAATTAAAGAAAATGCAGAAGCATTAAGCGGTGGTCAAAAGCAAAGATTAGCTATTGCTCGAGCAATAGCGCTAAGTCCAAAAGTACTTTTAATGGATGAACCTACTAGTGCGCTAGATCCAATTGCAACTCAAAAAATAGAGAACTTAATTTTAGATTTAAAAAATGATTACACCATTATTTTAGTAACTCACTCAATGGCTCAAGCTCAGAGAGTTAGTGATAAAACTGCATTCTTTTTAGCAGGTGAATTAATTGAAATTAATAGCACTAGAAACATATTTACTAAACCACTTAAAGAAGAAACAAAAGACTACATTATGGGAAAGATAGGATAG", "aa": "MNRIHGPQAGNYLEGKVEYLNQDLFSKKFINDIELRKQVGMIFQQPSVFPLSIYDNVAIGLRNIGIRDKKILDQKVQEALERAALYDEVKDKIKENAEALSGGQKQRLAIARAIALSPKVLLMDEPTSALDPIATQKIENLILDLKNDYTIILVTHSMAQAQRVSDKTAFFLAGELIEINSTRNIFTKPLKEETKDYIMGKIG", "aa_hexdigest": "fe4c4f74239b55db94544137b65ef815", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2N8PY15", "query_cov": 1.0, "subject_cov": 0.8055555555555556, "identity": 0.557, "score": 237.0, "evalue": 8.16e-76, "valid": false, "gene": "pstB", "product": "phosphate ABC transporter ATP-binding protein PstB", "ec_ids": [ "7.3.2.1" ], "uniref50_id": "UniRef50_P0A2V9", "go_ids": [ "GO:0005524", "GO:0006817", "GO:0009898", "GO:0015415", "GO:0055052" ] }, "pscc": { "uniref50_id": "UniRef50_P0A2V9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0A2V9" ], "product": "Phosphate import ATP-binding protein PstB 3" }, "genes": [ "pstB" ], "id": "OAIKOHHJFB_1013", "locus": "GCA000209735_00995" }, { "type": "cds", "sequence": "contig_1", "start": 554995, "stop": 555249, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGGCTAAAATCTTTTCAGAAACAACACCATTTATTTTAACTAGCGGACTAAGTAATTCAAAACATATTAGCTTATTATTATTTGGCCAAACATTAACAACTAGAATGAATGCTCAATTATCATTACCTTCTGCTTACTCAATTCCAACTATGTACTACTGTGCTATGATGTCATTTGTGACAATAGTTATCCTCTTTTCATTATCAGAATTTGTGATTCCAAAATTAAGCAAATTAAAAAGAAAACATTAG", "aa": "MLAKIFSETTPFILTSGLSNSKHISLLLFGQTLTTRMNAQLSLPSAYSIPTMYYCAMMSFVTIVILFSLSEFVIPKLSKLKRKH", "aa_hexdigest": "4dec9c3a8855e01ce8fb678273f9d50d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9449.254799999995, "isoelectric_point": 9.921055793762207 }, "id": "OAIKOHHJFB_1014", "locus": "GCA000209735_00996" }, { "type": "cds", "sequence": "contig_1", "start": 555385, "stop": 555690, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGCAACTTTAGAGCTTTATTTAACACTTTATATATCTCTTTAGTAGCAATTTTAATAACCTTCCCTTTTGCTTTTTTAGTAACAATTTATTTTGTTGAATTTAACAAAAGCAAAAAAGCAAAATTAGTATTTGATAACTTCATTTCAGTTATGACTTCAATGCCTGCTTTACTTTTTGGTGTCTTTGGTTCAGCTATCTTTTTAGGAACATTTCAATTGGCTTTAGGTGGTAAAACCAGTAACTCACTTTTAGCTGGAGCTTTAGCAATTAGCGTTATGTTATTTGCCTTCAGCTGTCGCTGA", "aa": "MGNFRALFNTLYISLVAILITFPFAFLVTIYFVEFNKSKKAKLVFDNFISVMTSMPALLFGVFGSAIFLGTFQLALGGKTSNSLLAGALAISVMLFAFSCR", "aa_hexdigest": "d563540ff53f872dd99ef91fa7232763", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11025.121799999988, "isoelectric_point": 9.899652290344235 }, "id": "OAIKOHHJFB_1015", "locus": "GCA000209735_00997" }, { "type": "cds", "sequence": "contig_1", "start": 555907, "stop": 556410, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAAAAATATATTTAATATGGAAAATGGGACAAACTTATTTAATGCCACAATGGTCTTAAGTTTAATGTGCATTCCAACTATGATCAATTCATTATTATTGCAATTTGAAAAAGTTGATTTATGTCTAAAACTCTCAGGTAGTGCTATTGGTTTAAGTGAATCAAAAGTAATTTATAAAATAATTAAAAAGTCATTAAACAAAAAAATATTTTTAATTTATATTAGCTCTTTTGCCAGAGTTATCGGTGAATCAGCTGCTTTAATGTTTATTTTAAATAGCAATAATTATTCAGAAAATTTTACAAATATTAAGAAATTTTTTAACTCTTCATTGCGTCCTCTTTCAGTATTTATTAATGCTAATTTCTTTGCTGAAAATGGTGGCGATGCAACTAGAAGTTACATGTTTAATTTAGCTTTTGTTCTATTAATTGTGATAACTTTAATTAATGCTGCAATCATTATTATTGTTAATTTAAAAGTCAAAAAACCTAAGTGA", "aa": "MNKNIFNMENGTNLFNATMVLSLMCIPTMINSLLLQFEKVDLCLKLSGSAIGLSESKVIYKIIKKSLNKKIFLIYISSFARVIGESAALMFILNSNNYSENFTNIKKFFNSSLRPLSVFINANFFAENGGDATRSYMFNLAFVLLIVITLINAAIIIIVNLKVKKPK", "aa_hexdigest": "db0b149afdec936373812f7fe8b69273", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18722.1494, "isoelectric_point": 9.791409873962401 }, "id": "OAIKOHHJFB_1016", "locus": "GCA000209735_00998" }, { "type": "cds", "sequence": "contig_1", "start": 556883, "stop": 557323, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAAGAAAAATCTTTTGCGAGCACTTGCCCCAATTTATTCCCTACCCTTAGTTACTTTTACAACCTCTTGTAACTTTTCAGTTATTAGTTTCAAGGGTTCAAGTAGCATGATTCAGTATTTAAATGATATTTCTAAAGAATTAGGTTATGAATATGAAATGAATATTGTAGGAGGAGGCAGTGGCACTGGCCTAAGTCAATTAATTCATGGCAAAACTGATTTTGCTTCAATAAGTAAAGATCCACGTTTTGGTTTGAATTCGTCAAAGAAAAAACAAGCTAAAAAAGAATATTTAGAAAATCAACTTAAGACTTTTTTAGTTGCAAAAGAAAATGTTGCTTTTGTACTTAAACTTCCTAAATCAAAGATTCAATTATCAAGTGGAGAAGTTGAAGCAAAAGATTTAAATGTTTATTTTAACAAAAATAATTGA", "aa": "MKKKKNLLRALAPIYSLPLVTFTTSCNFSVISFKGSSSMIQYLNDISKELGYEYEMNIVGGGSGTGLSQLIHGKTDFASISKDPRFGLNSSKKKQAKKEYLENQLKTFLVAKENVAFVLKLPKSKIQLSSGEVEAKDLNVYFNKNN", "aa_hexdigest": "d59e5789d143ab08865f42e83d5e5ab5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16238.613299999986, "isoelectric_point": 9.661248207092285 }, "id": "OAIKOHHJFB_1017", "locus": "GCA000209735_00999" }, { "type": "cds", "sequence": "contig_1", "start": 557360, "stop": 557593, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTTTAGCTGATATTACTTTAAATAATGATTCTAAATTAAAAGAATTTAACATTACTCCTTTTTATAAATCAGGAGGAGCAAACTTCAGCGGAACAGCTGAAGCTTTATCAAAACAAAATCCTTTTCTTGATATTAAACCAAGTGAAAAAGCAGCTAAATTTTTAGAAGATACAAGTGATAAACCAAAGTATGATGTCAAATTAGAAAATGATTCAAGTAATGAATTTTGA", "aa": "MTLADITLNNDSKLKEFNITPFYKSGGANFSGTAEALSKQNPFLDIKPSEKAAKFLEDTSDKPKYDVKLENDSSNEF", "aa_hexdigest": "6ffd9497766d16633e2882933a498a2a", "start_type": "TTG", "rbs_motif": "GGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8566.393699999999, "isoelectric_point": 4.791549873352051 }, "id": "OAIKOHHJFB_1018", "locus": "GCA000209735_01000" }, { "type": "cds", "sequence": "contig_1", "start": 557594, "stop": 557767, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAATGAAGCATTATGATAAACCATGTTTAGGCTTTTTTACCTTAGATTTTATTAAGAAAAATATAGAAGAGTTCAAACGATATAATTGCTTAGTTTTAAGTTATTTGAATCCTGATAATAACAAATATTATAGTCCTTTAAATGATCGTGAATTATTTGATTATGTATGA", "aa": "MSMKHYDKPCLGFFTLDFIKKNIEEFKRYNCLVLSYLNPDNNKYYSPLNDRELFDYV", "aa_hexdigest": "2aca2aa3ffb96819f2a44ba600bd40e4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6994.953500000001, "isoelectric_point": 6.528151512145995 }, "id": "OAIKOHHJFB_1019", "locus": "GCA000209735_01001" }, { "type": "cds", "sequence": "contig_1", "start": 559084, "stop": 559260, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAAAAGAAAAATAAATATTAAAGAAATTATTAAGGAATTCGAACTAACAATTTGTAATGAAGAATGTGATCCTATTTACCGAGATGTTCAAGAACCTGCGATTAAAAGATTAGGTTTAGAATTATCAGGTCAAATTGAAAACACTCGTTATTCAAGAAATATTATTTGTTGA", "aa": "MRKRKINIKEIIKEFELTICNEECDPIYRDVQEPAIKRLGLELSGQIENTRYSRNIIC", "aa_hexdigest": "7d727676140ae1a39f1dba749c1b15ad", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6925.002199999999, "isoelectric_point": 7.690281486511232 }, "id": "OAIKOHHJFB_1020", "locus": "GCA000209735_01002" }, { "type": "cds", "sequence": "contig_1", "start": 559236, "stop": 559460, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGATGTAGATGAGTTTTCAGTTGAGAAAACCGGTATTCTATATTTGTTGCAAACCTTTAAAATTCATTTTTTAGGTAACTCTCTAACCCCTTTTGAAAGAATAACTAAAGGCGGTTCTTCTTTAAGGACTCGAGCTAAAATGTTGATAGCTTCTTCTTTACCTTTTTTTGTAAAGAAAATTGATTCACTCGTACCTCAACAAATAATATTTCTTGAATAA", "aa": "MIDVDEFSVEKTGILYLLQTFKIHFLGNSLTPFERITKGGSSLRTRAKMLIASSLPFFVKKIDSLVPQQIIFLE", "aa_hexdigest": "773f5399c25e957ba0043d568d04dee5", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8418.863699999998, "isoelectric_point": 9.222669792175292 }, "id": "OAIKOHHJFB_1021", "locus": "GCA000209735_01003" }, { "type": "cds", "sequence": "contig_1", "start": 559702, "stop": 560025, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF66EA", "PFAM:PF07475.17" ], "nt": "TTGGAAGCTAGAGGTATTGGTTTGATTGATATGAAATATACTTATGGAGTTAAGTCAGTAACTGATAATTGTGAAATTGATTTAGTTGTTGAATTAGTAAGTAACCAAACAGAAGAAGCAAAAAATTTAGATCGCTTAGGTCTTTCAGATCTTAAGTTTCCAATTTTAGATGGAGCTTTACCTAAGATTGAAATTCCAGTTAAAGATGGTTCAAGTGCTGCTAGCTTAATCGAAGCAGCCGTAAGTACTTTCTTAGCAAGAAAAGATGGAATCGATGTTTTAAAACAAATACAAGAGAGAGGAGAAAAAGAACATGAATTATAA", "aa": "MEARGIGLIDMKYTYGVKSVTDNCEIDLVVELVSNQTEEAKNLDRLGLSDLKFPILDGALPKIEIPVKDGSSAASLIEAAVSTFLARKDGIDVLKQIQERGEKEHEL", "aa_hexdigest": "481b267776481435217f380f925715d1", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021AF66EA", "query_cov": 0.9065420560747663, "subject_cov": 0.9509803921568627, "identity": 0.546, "score": 103.0, "evalue": 9.78e-27, "valid": false, "uniref50_id": "UniRef50_UPI0021AF66EA" }, "pscc": { "uniref50_id": "UniRef50_UPI0021AF66EA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF66EA" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07475.17", "name": "Hpr_kinase_C", "length": 100, "aa_cov": 0.9345794392523364, "hmm_cov": 0.98, "evalue": 2.8525982749925204e-25, "score": 84.87510681152344, "start": 1, "stop": 100 } ], "seq_stats": { "molecular_weight": 11715.26409999999, "isoelectric_point": 4.65905818939209 }, "id": "OAIKOHHJFB_1022", "locus": "GCA000209735_01004" }, { "type": "cds", "sequence": "contig_1", "start": 560339, "stop": 560455, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCCTTTGTACTTGACATGATTTATGTTTATACCAAAAGAGATCTAGTTGACATTCGTAAAGTTGCTTCAATTATTATTCCAACAATTCTAATTGGTCAAGTTATTGGACGTTGA", "aa": "MAFVLDMIYVYTKRDLVDIRKVASIIIPTILIGQVIGR", "aa_hexdigest": "327c3a5344c25d6e7f5528bd1bc1e2af", "start_type": "TTG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4335.2679, "isoelectric_point": 9.518902015686034 }, "id": "OAIKOHHJFB_1023", "locus": "GCA000209735_01005" }, { "type": "cds", "sequence": "contig_1", "start": 560543, "stop": 560641, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATTAGCGATGATATTTCAAAAGGCGCTTATCGTTATCCTCTCTTCTTATATGAATCTCTTGCTAACTTAGTTGGTTACATTGTTTTAGTATGA", "aa": "MFISDDISKGAYRYPLFLYESLANLVGYIVLV", "aa_hexdigest": "194cdaf7206032b834f7b6ca8cb56998", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3671.260400000001, "isoelectric_point": 4.556577491760254 }, "id": "OAIKOHHJFB_1024", "locus": "GCA000209735_01006" }, { "type": "cds", "sequence": "contig_1", "start": 560723, "stop": 560848, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACCATTGCGTGAAGACCAATACCACATGTATACAATAACCTCTATTGTATTTATTGGTTTAGGTGGTTTAGCTTTTGTCTTCTTTGAATTCTTCAATCCAACTCACTATGTTCGTGTTTGA", "aa": "MEPLREDQYHMYTITSIVFIGLGGLAFVFFEFFNPTHYVRV", "aa_hexdigest": "7aeeeeb78b8d297c738c663332214a42", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4886.6017, "isoelectric_point": 5.34811725616455 }, "id": "OAIKOHHJFB_1025", "locus": "GCA000209735_01007" }, { "type": "cds", "sequence": "contig_1", "start": 560964, "stop": 561896, "strand": "+", "frame": 3, "gene": null, "product": "FAD/NAD(P)-binding domain-containing protein", "db_xrefs": [ "RefSeq:WP_013527129.1", "SO:0001217", "UniParc:UPI0001F598DF", "UniRef:UniRef100_C4XF47", "UniRef:UniRef50_C4XF47", "UniRef:UniRef90_C4XF47" ], "nt": "ATGGACAAAAAAGATATTAAAAAATTTGATGTTTTAATTGTTGGATCAGGACCCGCAGGACTAAATGCTGCTCTTTATACCTCTCGAGCAGGACTAAATACAGCTTTTATTGATAATGATGTCTTTGGTGGCAAAATTCAAAAAACAGCTAAGGTAGATAACTACCTTGGAATTCAAGATATAAGTGGTTTTGACTTATCACAAAAATTTTACGAACATGCCTTAAAATATGGCGCAAAACATCTTTATGGCAAGGTTGTAAAAATCAATTCAATTAATGAAAATTTACAAGAAGCAATTCTTGAAAATGGTGAAATTATTCAAGCTAAAAAATTAATTATAGCTTCAGGTATGATTAATAGATATCCTAAAGGTGTTGATAATTATCAAAGACTTGAAGAATATGTAAGCTTTTGTGCTATTTGCGATGGTGCATTATATAAGAATAGAAAAGTAGTTGTATTAGGCGGCGGAAATTCTGCAGTTGAAGAAGGAACTTTTTTAGCTAAATTATGTACTGATGTAACAATAGTTACAAATCAAAATACATTTAGTGCTGAGGAAGAATTGGTTGAGGAACTCAAAAAGCAAAAAAATGCTAAAATTTATTATGATCATGAATTAATTTCTTTAAATGGTGAAAATCAGTTAACTTCAGTAACTATAAGGAATAAAAAAACCAATAAAGAAATTAATATTGTAGCTGATGGCTTATTTGTTTTCATAGGCTTTATACCAAACCAAGATGCATTTAAGCATCTAAAAATAACAACTAAAGAAGGTTTTGTAAAAACCAATGAAGATATGGAAACAAAAGTAAAAGGCATCTATGCAATTGGTGATATCAGAGATAAAAAAGTAAGACAAATTATTACAGCAGCTGCTGATGGTGCAATAGCTGCAAAACACATTTCTGATACATTAAAAAATTAG", "aa": "MDKKDIKKFDVLIVGSGPAGLNAALYTSRAGLNTAFIDNDVFGGKIQKTAKVDNYLGIQDISGFDLSQKFYEHALKYGAKHLYGKVVKINSINENLQEAILENGEIIQAKKLIIASGMINRYPKGVDNYQRLEEYVSFCAICDGALYKNRKVVVLGGGNSAVEEGTFLAKLCTDVTIVTNQNTFSAEEELVEELKKQKNAKIYYDHELISLNGENQLTSVTIRNKKTNKEINIVADGLFVFIGFIPNQDAFKHLKITTKEGFVKTNEDMETKVKGIYAIGDIRDKKVRQIITAAADGAIAAKHISDTLKN", "aa_hexdigest": "41bc8ac8a4d9758835c2e67e1bf6a022", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001F598DF", "ncbi_nrp_id": "WP_013527129.1", "uniref100_id": "UniRef100_C4XF47", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F598DF", "RefSeq:WP_013527129.1", "UniRef:UniRef100_C4XF47" ] }, "ips": { "uniref100_id": "UniRef100_C4XF47", "uniref90_id": "UniRef90_C4XF47" }, "psc": { "uniref90_id": "UniRef90_C4XF47", "product": "FAD/NAD(P)-binding domain-containing protein", "uniref50_id": "UniRef50_C4XF47" }, "pscc": { "uniref50_id": "UniRef50_C4XF47", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF47" ], "product": "FAD/NAD(P)-binding domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_1026", "locus": "GCA000209735_01008" }, { "type": "cds", "sequence": "contig_1", "start": 561909, "stop": 562292, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF48", "UniRef:UniRef90_C4XF48" ], "nt": "ATGTTTAGAACTTTAAAAGAAAGATTTATAACATTAACAGTAATGTTTGCTCTTGCTTCATTAATGACTATTATAGCTATGGCATTATTTGGAGTTTCAAGAATTGTATTCACTACTTTAGGTGGTGTTACATCCTTAGGAATGTCAATAGCAGCATTTATCTTAGCAATTATAGCCTTTGTCTTTGTTCTTATTATTTACTTGCTTTACAATAGAATAATGGCAAAACGTACAGAAGATTTTAGTCGTTTTAGACACACAAAAGCATCACAAATTTTTGTTTTAGTAGCTTTAATTTGTTATGCTGTTGCAGCAGTTACAGCAATTTTGTATTTTGTAATGGTAGCTATTGTTCATAATAATAACTCAAGTACAATTAGTTGA", "aa": "MFRTLKERFITLTVMFALASLMTIIAMALFGVSRIVFTTLGGVTSLGMSIAAFILAIIAFVFVLIIYLLYNRIMAKRTEDFSRFRHTKASQIFVLVALICYAVAAVTAILYFVMVAIVHNNNSSTIS", "aa_hexdigest": "673262d270170ab64c1d70a6fb802551", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 561909, "stop": 562445, "observations": { "mutation": [ 562290, 562335, 562356 ], "stop": [ 562290, 562335, 562356 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF48", "query_cov": 1.0393700787401574, "subject_cov": 0.7213114754098361, "identity": 0.9390000000000001, "score": 204.0, "evalue": 5.22e-65, "gene_start": 1, "gene_end": 127, "reference_start": 1, "reference_end": 132, "reference_sequence": "MFRTLKERFITLTVMFALASLMTIIAMALFGVSRIAFATLSGVVIGVRTSLGMSIAAFILAIIAFVFVLIIYLLYNRIMAKRTEDFSRFRHTKASQIFVLVALICYAVAAVTAILYFVMVAIVHNNNSSTISWETVRAFSIVFEIFFWIGIVIGWLFIILYYVFFCIIVDNITRYRRAQARKK" }, "paralog": false, "identity": 0.9398907103825137, "score": 313.0, "evalue": 1.04e-110, "description": "Internal stop codon at 562290, 562335, 562356. Nonsense mutation around 562290, 562335, 562356.", "psc": { "uniref90_id": "UniRef90_C4XF48", "uniref50_id": "UniRef50_C4XF48", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF48", "UniRef:UniRef50_C4XF48" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF48", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF48" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 14112.980199999978, "isoelectric_point": 10.363308906555172 }, "id": "OAIKOHHJFB_1027", "locus": "GCA000209735_01009" }, { "type": "cds", "sequence": "contig_1", "start": 562553, "stop": 562753, "strand": "+", "frame": 2, "gene": "rpmB", "product": "50S ribosomal protein L28", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354947.1", "SO:0001217", "UniParc:UPI0001E32FEB", "UniRef:UniRef100_C4XF49", "UniRef:UniRef50_Q98PM6", "UniRef:UniRef90_A0A3P8MF76" ], "nt": "ATGTCAAGAAGAGACCAATTAACAGGCAAAGGACCTATGGTTGGAAACATTCGTTCACACGCAATGAATGCTTCAAAAAGAAAATTTAACGTTAACTTGCAAAAAGTAACAATGAATGTTAATGGTAAAAAAGTAACTTTAAGAGTTAGTGCTAAAACAGCAAAAACCTTAAAGAACAAAGGTGTTAGTGTTGTTGCCTAA", "aa": "MSRRDQLTGKGPMVGNIRSHAMNASKRKFNVNLQKVTMNVNGKKVTLRVSAKTAKTLKNKGVSVVA", "aa_hexdigest": "67da65a9a58e544d6c8369b3b20a907f", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32FEB", "ncbi_nrp_id": "WP_013354947.1", "uniref100_id": "UniRef100_C4XF49", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FEB", "RefSeq:WP_013354947.1", "UniRef:UniRef100_C4XF49" ] }, "ips": { "uniref100_id": "UniRef100_C4XF49", "uniref90_id": "UniRef90_A0A3P8MF76" }, "psc": { "uniref90_id": "UniRef90_A0A3P8MF76", "gene": "rpmB", "product": "50S ribosomal protein L28", "uniref50_id": "UniRef50_Q98PM6", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PM6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PM6" ], "product": "Large ribosomal subunit protein bL28" }, "genes": [ "rpmB" ], "id": "OAIKOHHJFB_1028", "locus": "GCA000209735_01010" }, { "type": "cds", "sequence": "contig_1", "start": 562871, "stop": 563416, "strand": "-", "frame": 1, "gene": null, "product": "Ribulose-phosphate 3-epimerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6T1", "UniRef:UniRef90_C4XF50" ], "nt": "ATGGATGGCAAATTTGTACCTAACACAGCTATTGAATTAGATGAAATTAAAAACATTAACAAGAATGCACCTAAACACTTTAAAGATGCTCACTTAATGGTTGAAAATCCTCTTGATTACTTAGATGAATTTAGAGACCATGTTGATATTACAACAATTCATTATGAAGCAGTTAATAAAAAAGAATTGTTGCAATTCTTAAAAGATCATCATTCAGCTTACAGTATAGGACTTGCTATTAAACCAGCAACCGAAGTTAAAGAAATAGTTAAAATACTTCCTTATTTACAACTTGTTTTAGTTATGTCTGTAGAACCTGGCAAGGGAGGACAAAAATTTATTGAACATGCTGTTGATAAAATTAGAGAACTTAAACAATTAAGAGACAAAAATGGTTACAAATATTTAATTCAAGTTGATGGTGGAATTAATGGTGAAACTGGACCAAGATGTTTTGCAGCTGGAGTAGATGCAACAGTAGCTGGAACTTATATTGTTTCAGAACCAACTAAGGAAAGAATTGCTAGTGTATTTGGTAAAAAATAA", "aa": "MDGKFVPNTAIELDEIKNINKNAPKHFKDAHLMVENPLDYLDEFRDHVDITTIHYEAVNKKELLQFLKDHHSAYSIGLAIKPATEVKEIVKILPYLQLVLVMSVEPGKGGQKFIEHAVDKIRELKQLRDKNGYKYLIQVDGGINGETGPRCFAAGVDATVAGTYIVSEPTKERIASVFGKK", "aa_hexdigest": "277d2a4d1646201ef23da892ae2f059b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XF50", "query_cov": 1.0, "subject_cov": 0.8153153153153153, "identity": 1.0, "score": 366.0, "evalue": 1.31e-127, "valid": true, "product": "Ribulose-phosphate 3-epimerase", "uniref50_id": "UniRef50_Q4A6T1" }, "pscc": { "uniref50_id": "UniRef50_Q4A6T1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6T1" ], "product": "Ribulose-phosphate 3-epimerase" }, "genes": [], "id": "OAIKOHHJFB_1029", "locus": "GCA000209735_01011" }, { "type": "cds", "sequence": "contig_1", "start": 563432, "stop": 563533, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAAAAGAAAAATATGTAACACCTTCATTATTAAATGTTGAAAAAGATAAACGTTGTGAAATGGCAACACTATTAGTTAATAATGGAATTAAATGA", "aa": "MNKKEKYVTPSLLNVEKDKRCEMATLLVNNGIK", "aa_hexdigest": "7d565ecce1ab86c360dfabbc5514f727", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3808.4932, "isoelectric_point": 9.360438728332518 }, "id": "OAIKOHHJFB_1030", "locus": "GCA000209735_01012" }, { "type": "cds", "sequence": "contig_1", "start": 563526, "stop": 563903, "strand": "-", "frame": 3, "gene": null, "product": "ribosome small subunit-dependent GTPase A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013679F51" ], "nt": "ATGGGTCAAAGCGGAGTTGGAAAAACTACAATAATTAATAAATTAACCAACAATAATTATGAAACTCAAAACATTTCAAAATCAGTAAATCGGGGTAAACATACCACAAGAATTGTTCAAATAATTCCAATATTAAATGGTGAATTAATTGACACTCCAGGCTTTAGCTCTTTTGATTTAAACATCTCAAAATTAGAACTTGCTTCTTCTTATTCTCAATTTAGAGAGCTAGGAAAATTATGTAAATTTAAATCATGCTTGCATGAAAATGAACCTGAAGAATATTGCAATATAAAACTCAATGTAAAAAACAAAACAATACCAGAATTTAGATATCAAAATTATCTAAAATTATTAAAGGAGATTAAACATGAATAA", "aa": "MGQSGVGKTTIINKLTNNNYETQNISKSVNRGKHTTRIVQIIPILNGELIDTPGFSSFDLNISKLELASSYSQFRELGKLCKFKSCLHENEPEEYCNIKLNVKNKTIPEFRYQNYLKLLKEIKHE", "aa_hexdigest": "54255a8149dd775762fd9cdb3e183f00", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0013679F51", "query_cov": 1.0, "subject_cov": 0.8865248226950354, "identity": 0.552, "score": 144.0, "evalue": 6.83e-42, "valid": false, "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "ec_ids": [ "3.1.3.100", "3.6.1.-" ], "uniref50_id": "UniRef50_UPI0013679F51", "kegg_orthology_id": "K06949", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0006412", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0013679F51", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013679F51" ], "product": "ribosome small subunit-dependent GTPase A" }, "genes": [], "id": "OAIKOHHJFB_1031", "locus": "GCA000209735_01013" }, { "type": "cds", "sequence": "contig_1", "start": 563949, "stop": 564362, "strand": "-", "frame": 3, "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "db_xrefs": [ "EC:3.1.3.100", "EC:3.6.1.-", "GO:0003924", "GO:0005525", "GO:0006412", "GO:0043022", "KEGG:K06949", "SO:0001217", "UniRef:UniRef50_L0RWK0", "UniRef:UniRef90_C4XF51" ], "nt": "TTGAAAGGCAAAATTTATTCAATTAATTCAGGTAAATATTACATAAATGATTTGCAAAATAAAATGCACATATTACCTGCAGCTGGTGTTTTTCGTCACAAAGAAATAACACCTTTAGTGGGTGATATTGTTGAGTTTGAAGAAGGCCAATATATCAAAGAAATATATCCTAGAAAAAATTCTTTTGTTCGTCCAAAAGTAGCAAACATCGACAATATTGTTGTGGTTATGTCAATTAAAGAACCTGAATTTCAAAGTTACTTAGTTGACAAATATTTAGCTTTTATAGAAAGTAAAAATATCAAACCAATTATCATTTTGACTAAAGATGATTTAGGTTCAACCCCTTACTTTGAACAATATAAAAATATGGGTTATGAAATTTACCAAATAAACTATAAAATAAATGATTGA", "aa": "MKGKIYSINSGKYYINDLQNKMHILPAAGVFRHKEITPLVGDIVEFEEGQYIKEIYPRKNSFVRPKVANIDNIVVVMSIKEPEFQSYLVDKYLAFIESKNIKPIIILTKDDLGSTPYFEQYKNMGYEIYQINYKIND", "aa_hexdigest": "4ec913c3114f4729477e10c9da87fa97", "start_type": "TTG", "rbs_motif": "AGGAG", "genes": [ "rsgA" ], "truncated": "5-prime", "pseudogene": { "start": 563646, "stop": 564362, "observations": { "mutation": [ 563951 ], "stop": [ 563951 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF51", "query_cov": 1.0, "subject_cov": 0.49280575539568344, "identity": 1.0, "score": 279.0, "evalue": 2.7e-93, "gene_start": 1, "gene_end": 137, "reference_start": 1, "reference_end": 137, "reference_sequence": "MKGKIYSINSGKYYINDLQNKMHILPAAGVFRHKEITPLVGDIVEFEEGQYIKEIYPRKNSFVRPKVANIDNIVVVMSIKEPEFQSYLVDKYLAFIESKNIKPIIILTKDDLGSTPYFEQYKNMGYEIYQINYKINDWVKIFTKIFDHKTCSLMGQSGVGKTTIINKLTNNNYETQNISKSVNRGKHTTRIVQIIPILNGELIDTPGFSSFDLNISKLELASSYSQFRELGKLCKFKSCLHENEPEEYCNIKLNVKNKTIPEFRYQNYLKLLKEIKHE" }, "paralog": false, "identity": 0.9915966386554622, "score": 473.0, "evalue": 6.79e-172, "description": "Internal stop codon at 563951. Nonsense mutation around 563951.", "psc": { "uniref90_id": "UniRef90_C4XF51", "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "ec_ids": [ "3.1.3.100", "3.6.1.-" ], "uniref50_id": "UniRef50_L0RWK0", "kegg_orthology_id": "K06949", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0006412", "GO:0043022" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF51", "EC:3.1.3.100", "EC:3.6.1.-", "UniRef:UniRef50_L0RWK0", "KEGG:K06949", "GO:0003924", "GO:0005525", "GO:0006412", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_L0RWK0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RWK0" ], "product": "Small ribosomal subunit biogenesis GTPase RsgA" } }, "id": "OAIKOHHJFB_1032", "locus": "GCA000209735_01014" }, { "type": "cds", "sequence": "contig_1", "start": 564375, "stop": 564566, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTCGTGATCTTATGACTTGTCTTGATACTAAAAGAGTTTATGAAAAACCTCTTGATCTTAGAAAAGTTAAACCTAAAAACACAATTCAAGAAAAAGTTAATAGCAAGAAATTTATTATTGCAGGTATTTCAATAATATTAGTTGTTTTGATTATAGCTTTAGTATTGATTGTTGTTTATGTTCACTAA", "aa": "MRRDLMTCLDTKRVYEKPLDLRKVKPKNTIQEKVNSKKFIIAGISIILVVLIIALVLIVVYVH", "aa_hexdigest": "8ad71a6af2053b45477d0ecbc3bec0a7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7290.9175, "isoelectric_point": 10.008668327331542 }, "id": "OAIKOHHJFB_1033", "locus": "GCA000209735_01015" }, { "type": "cds", "sequence": "contig_1", "start": 564570, "stop": 565373, "strand": "-", "frame": 3, "gene": null, "product": "Protein kinase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B4H5", "UniRef:UniRef90_C4XF52" ], "nt": "ATGGGAATTAAACCACAAAGTAATATATTTAAGAAATATCGTGTGCTATCAACTATTGGTGATGGTGATTTTAGTCAAGTTTATAAAGTTGAACTTTTAAATGATAAAAGCCCAATTAAAAGAAAGTTTGCACTTAAATATAGTGTTAATAAAAATAAAAATGATGAAGACATAACTCGTAGAAGATTTGAACAAGAAATCACGATATACAAAAAATTAAATACTGAACGTGTGGCATTATACTTTGACTCTTATAGCGATGAATATGAACAATATCTTGTAATGGAATTTGTTGAAGGACAAAATTTAAGAGAAATAATTAAAAGAAATGGTAAATTCACTACAACAGTTGCTGTTAACTATGCAATTCAAATAGCTGAAGGAATTAGTGAATTACATAACTTAGGTGTAATTCACCGTGATATTAAAAGTAATAACATTTTAATTACCAAAGATAAAAATGTAAAAATTATTGACCTTGGCCTAGCTTTAGATGATGAAAGTCAAAGACTTACACAAGAAAACAAAGTAGTCGGTTCTGTTTTCTATATGGCTCCTGAAATTTGTTTAGCTAATTCGAAACCATCTGTCCGTTCGGACATTTATGCTTTAGGCATTTTGTTATTTGAAATGCTAACTGGCTCATATCCTATTTCAGGTAAAGACCAACAAGAAACTTTAAGAATGCAAAGAACAACAAATGTTCCTAACTTATTAAACTTTGTTAATGCACCTCAAGCTTTAGCTAACGTTATCATCAAAGCAACAGCTAAAGATCCAAACAAAAGATATGCAACTATGTGA", "aa": "MGIKPQSNIFKKYRVLSTIGDGDFSQVYKVELLNDKSPIKRKFALKYSVNKNKNDEDITRRRFEQEITIYKKLNTERVALYFDSYSDEYEQYLVMEFVEGQNLREIIKRNGKFTTTVAVNYAIQIAEGISELHNLGVIHRDIKSNNILITKDKNVKIIDLGLALDDESQRLTQENKVVGSVFYMAPEICLANSKPSVRSDIYALGILLFEMLTGSYPISGKDQQETLRMQRTTNVPNLLNFVNAPQALANVIIKATAKDPNKRYATM", "aa_hexdigest": "ce25b8531044c041a076e2fa892663eb", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_C4XF52", "query_cov": 1.0, "subject_cov": 0.8042168674698795, "identity": 0.996, "score": 514.0, "evalue": 3.43e-183, "valid": true, "product": "Protein kinase domain-containing protein", "uniref50_id": "UniRef50_A0A449B4H5" }, "pscc": { "uniref50_id": "UniRef50_A0A449B4H5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B4H5" ], "product": "Serine/threonine protein kinase" }, "genes": [], "id": "OAIKOHHJFB_1034", "locus": "GCA000209735_01016" }, { "type": "cds", "sequence": "contig_1", "start": 565375, "stop": 565884, "strand": "-", "frame": 2, "gene": null, "product": "Serine/threonine phosphatase stp", "db_xrefs": [ "EC:3.1.3.16", "KEGG:K20074", "SO:0001217", "UniRef:UniRef50_A0A449B4I9", "UniRef:UniRef90_A0A3P8KWN9" ], "nt": "ATGATTAAAATTAGTGCTAATGATGAAGCAAAACTAGACATGGGAACAACAGTTACAGCTGCCTTACTTAATACAAAAACAAAATTATTATTAATATTTAATATTGGCGATTCACGTACTTATGCTATGACAACTTTTGGTGAATTAAAGCAAATTTCAATTGATCACAACTTATACAATAAGTTAATTAATGAAGAAGGTAAAAGTCCTTATGAGGCTAAATTGAATCGTTTCCATGCTTCATTAACTTCAGCTTTAGGTCCTGATAAAAAAACTAAAATTGAAGTTTTTGACTTAAGTAGTCAATACGAACATGTTTATAGTTTATTAGTCACAAGTGATGGTGTACACGACTTTATCGAAAAACCAAAAATGGAAATGATTTTAAGGGAAAATCTTAATGCTGAAGACATGACTAAAAAATTAGTGAAAGAGGCTTTAGATAATAAATCAACAGATAACTGCTCAGCAGGTATGGTCGTATTAGACAATCTAAAAGAATGGAGTTAA", "aa": "MIKISANDEAKLDMGTTVTAALLNTKTKLLLIFNIGDSRTYAMTTFGELKQISIDHNLYNKLINEEGKSPYEAKLNRFHASLTSALGPDKKTKIEVFDLSSQYEHVYSLLVTSDGVHDFIEKPKMEMILRENLNAEDMTKKLVKEALDNKSTDNCSAGMVVLDNLKEWS", "aa_hexdigest": "8a070b0d086ff4eb5b95e98eabab5063", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 565375, "stop": 566157, "observations": { "mutation": [ 565920 ], "stop": [ 565920 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8KWN9", "query_cov": 1.0, "subject_cov": 0.65, "identity": 1.0, "score": 328.0, "evalue": 1.82e-112, "gene_start": 1, "gene_end": 169, "reference_start": 92, "reference_end": 260, "reference_sequence": "MRYQIMEYGITSDMGRVRLENQDKASYFTKGNYVLLILCDGMGGHYGGGIASRTTINTFNKIFNNSIPAENSEIKDYVSWFKKTVDASRDEMIKISANDEAKLDMGTTVTAALLNTKTKLLLIFNIGDSRTYAMTTFGELKQISIDHNLYNKLINEEGKSPYEAKLNRFHASLTSALGPDKKTKIEVFDLSSQYEHVYSLLVTSDGVHDFIEKPKMEMILRENLNAEDMTKKLVKEALDNKSTDNCSAGMVVLDNLKEWS" }, "paralog": false, "identity": 0.9923076923076923, "score": 512.0, "evalue": 3.58e-187, "description": "Internal stop codon at 565920. Nonsense mutation around 565920.", "psc": { "uniref90_id": "UniRef90_A0A3P8KWN9", "product": "Serine/threonine phosphatase stp", "ec_ids": [ "3.1.3.16" ], "uniref50_id": "UniRef50_A0A449B4I9", "kegg_orthology_id": "K20074", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8KWN9", "EC:3.1.3.16", "UniRef:UniRef50_A0A449B4I9", "KEGG:K20074" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449B4I9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B4I9" ], "product": "Serine/threonine phosphatase stp" } }, "id": "OAIKOHHJFB_1035", "locus": "GCA000209735_01017" }, { "type": "cds", "sequence": "contig_1", "start": 565918, "stop": 566142, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATATGGAATAACAAGCGACATGGGAAGAGTAAGGTTGGAAAACCAAGACAAAGCTAGTTACTTTACAAAAGGTAACTATGTTTTATTAATTTTATGTGATGGAATGGGTGGTCACTATGGTGGTGGGATTGCTTCAAGGACAACAATAAATACTTTTAATAAAATTTTTAACAATTCAATTCCAGCTGAAAATTCTGAAATTAAAGATTATGTATCTTGA", "aa": "MEYGITSDMGRVRLENQDKASYFTKGNYVLLILCDGMGGHYGGGIASRTTINTFNKIFNNSIPAENSEIKDYVS", "aa_hexdigest": "60c8b73bca8efe0ae567b04e59b7b3b5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8188.092699999998, "isoelectric_point": 5.636006736755371 }, "id": "OAIKOHHJFB_1036", "locus": "GCA000209735_01018" }, { "type": "cds", "sequence": "contig_1", "start": 566142, "stop": 566750, "strand": "-", "frame": 3, "gene": "gmk", "product": "guanylate kinase", "db_xrefs": [ "EC:2.7.4.8", "GO:0004385", "GO:0006163", "SO:0001217", "UniRef:UniRef50_Q4A6S7", "UniRef:UniRef90_C4XF54" ], "nt": "ATGAATATTATGAACAATTTAATTAGCAAACGTAAGCCAATAATAATATTTACAGGACCTAGCGGAGTTGGCAAAGGAACAATTGAAAAGTACCTTTTTGATTTTAAAAATTTAAAATTACATTTATCATGTTCAGCAACAACTAGAGCTCCTCGTGAAGGTGAAATTAATGGCATTCATTACTTTTTTATAACAAAAGAAGAATTTGAAAAACACATTCAAAATGATGATCTTTTTGAATACAGTTTTCATTTTGATAATTATTATGGAACACTTTATAGTGAAATTGCAAGAATTCATAGAAATGGCGAAATTCCTGTTTTAGAAATTGAAACCAATGGTGCAAAACAAATATTAGAAAAGGTTTCAAATAACAAGAATTACAAATTGATCACACTTTTTATCTTACCTCCTTCAATTGAAGAACTTGAAAGAAGAATTATTAACCGAAAGACAGAAAGTCAAGAAGCTATTAAAAAACGTTTAGCTAAAGCGCAAGCAGAAATTGCAGAAAAAGGAATATTTAGACATCACATAATTAATGATGACCCTTTAAGAGCTGCAAAAGAAATAAAAGAAATTATAGAGAAAGAATACGGTATCAAATAA", "aa": "MNIMNNLISKRKPIIIFTGPSGVGKGTIEKYLFDFKNLKLHLSCSATTRAPREGEINGIHYFFITKEEFEKHIQNDDLFEYSFHFDNYYGTLYSEIARIHRNGEIPVLEIETNGAKQILEKVSNNKNYKLITLFILPPSIEELERRIINRKTESQEAIKKRLAKAQAEIAEKGIFRHHIINDDPLRAAKEIKEIIEKEYGIK", "aa_hexdigest": "d2c1cadc72c5d57840c14dfdae4cea65", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XF54", "query_cov": 1.0, "subject_cov": 0.9528301886792453, "identity": 1.0, "score": 397.0, "evalue": 9.73e-140, "valid": true, "gene": "gmk", "product": "guanylate kinase", "ec_ids": [ "2.7.4.8" ], "uniref50_id": "UniRef50_Q4A6S7", "go_ids": [ "GO:0004385", "GO:0006163" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A6S7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6S7" ], "product": "Guanylate kinase" }, "genes": [ "gmk" ], "id": "OAIKOHHJFB_1037", "locus": "GCA000209735_01019" }, { "type": "cds", "sequence": "contig_1", "start": 566840, "stop": 566974, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAGACTATATTGCACTTTCAAAAGCAACAGTTGAAAAATATGTTATGCCAATGATATTCTACTTATTTAATGAAATTAATGACACCAAAGTATTTAATGAAAAAACTCTTTCAGATTTATACGAAATTTAA", "aa": "MVDYIALSKATVEKYVMPMIFYLFNEINDTKVFNEKTLSDLYEI", "aa_hexdigest": "6448879fe5c36b8893f9243f0f1e2b57", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5241.0548, "isoelectric_point": 4.440682792663575 }, "id": "OAIKOHHJFB_1038", "locus": "GCA000209735_01020" }, { "type": "cds", "sequence": "contig_1", "start": 567203, "stop": 568135, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF55", "UniRef:UniRef90_C4XF55" ], "nt": "ATGAAAAACAAAATAACCAAACACAATATGTGTGAAATTTATGAGTCATTTCGTTTTATTCAACCTGAATATAAAAGTCGTGTAAATTCATTAAAAAATAAAAATGAAAATGACGAATTTTTAATCAAAGCACAAATGGAAAGTGCTATTTCATTGAATGAATATATTGATTCAAAATTAGAAAGAAAAAAGATCAGAATTGCTAATCGTAAGATTTCAAAAATCTCAAGTATCTTTATTCCACTAATTGTTTTCTTCTTAGTAACTGTTTTAATTGCAGTGTCTTATATTGCTTATGTAGCAGCTAACTATATGGTTAAAGATGCTGAAGTTTATACACTATTTGGTATAGCTATATTATCACTTATTTCAGCAGCAATATGCTTTGTAGTTTACTATAGAAAATACACGCGATATAACAATGATAAGGCTAAATATTTTGAAGATAAATTAAAAGAAGAAATCAAAATTGGAGATTTAAAATCTATAGTTTTAAAAAACAAAAATACATTAAATTATCAAGAAGATGTTGCTTCATTAGTTGTTGCTAACGATCTAAAATATCCTGATATTCCTGCAGGCTACAAAGTTGAAGCTTCAAAATACGCTAAACGTTTTACACTATATGGAATGAATGTTTTAGCACAAGATGCTCAACTAGTAAAAATTGACGAAAAGGCTAATGAAACAGCTGAATCAGACAAAAGTATTATTAGTAATAAAGCTAAAAAAATTAAGATTTTTGAATTCTCATTTATGAATAGTCAATTTGACAAAATTTATTTACCATTTAACTTATTTAACAAATTTGAAGTTTTCAAAGCTAAACTTGAACCAATCAAAAGTAACGTTTCTGATTTAGACAAAATCTTCAATATTCATGCAATCAAAAACCCGCTTAACAGTAAACTTTTCGATGCTCTTAAATTATGA", "aa": "MKNKITKHNMCEIYESFRFIQPEYKSRVNSLKNKNENDEFLIKAQMESAISLNEYIDSKLERKKIRIANRKISKISSIFIPLIVFFLVTVLIAVSYIAYVAANYMVKDAEVYTLFGIAILSLISAAICFVVYYRKYTRYNNDKAKYFEDKLKEEIKIGDLKSIVLKNKNTLNYQEDVASLVVANDLKYPDIPAGYKVEASKYAKRFTLYGMNVLAQDAQLVKIDEKANETAESDKSIISNKAKKIKIFEFSFMNSQFDKIYLPFNLFNKFEVFKAKLEPIKSNVSDLDKIFNIHAIKNPLNSKLFDALKL", "aa_hexdigest": "39f1f78ea27be4cedf8b794798c5ee14", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 566900, "stop": 568150, "observations": { "mutation": [ 567001, 567064, 567205 ], "stop": [ 567001, 567064, 567205 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF55", "query_cov": 1.0, "subject_cov": 0.7110091743119266, "identity": 1.0, "score": 577.0, "evalue": 1.26e-205, "gene_start": 1, "gene_end": 310, "reference_start": 6, "reference_end": 315, "reference_sequence": "MYGVSMKNKITKHNMCEIYESFRFIQPEYKSRVNSLKNKNENDEFLIKAQMESAISLNEYIDSKLERKKIRIANRKISKISSIFIPLIVFFLVTVLIAVSYIAYVAANYMVKDAEVYTLFGIAILSLISAAICFVVYYRKYTRYNNDKAKYFEDKLKEEIKIGDLKSIVLKNKNTLNYQEDVASLVVANDLKYPDIPAGYKVEASKYAKRFTLYGMNVLAQDAQLVKIDEKANETAESDKSIISNKAKKIKIFEFSFMNSQFDKIYLPFNLFNKFEVFKAKLEPIKSNVSDLDKIFNIHAIKNPLNSKLFDALKLWKNNIHFAHTFIPQESGVNYFNFKLTAIFTLKEECEDEFNIQTNAKYWNNPFKVELPETIDDSTKLPMWFIINNFYKMVDYIALSKATVEKYVMPMIFYLFNEINDTKVFNEKTLSDLYEI" }, "paralog": false, "identity": 0.9903846153846154, "score": 806.0, "evalue": 2.78e-298, "description": "Internal stop codon at 567001, 567064, 567205. Nonsense mutation around 567001, 567064, 567205.", "psc": { "uniref90_id": "UniRef90_C4XF55", "uniref50_id": "UniRef50_C4XF55", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF55", "UniRef:UniRef50_C4XF55" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF55", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF55" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 35912.49940000003, "isoelectric_point": 9.37848987579346 }, "id": "OAIKOHHJFB_1039", "locus": "GCA000209735_01021" }, { "type": "cds", "sequence": "contig_1", "start": 568349, "stop": 568585, "strand": "+", "frame": 2, "gene": "secG", "product": "preprotein translocase subunit SecG", "db_xrefs": [ "GO:0015136", "GO:0015739", "GO:0016020", "RefSeq:WP_013354940.1", "SO:0001217", "UniParc:UPI0001E32FE4", "UniRef:UniRef100_C4XF56", "UniRef:UniRef50_A0A4Y6I792", "UniRef:UniRef90_C4XF56" ], "nt": "ATGGCAGTTATATTTGTATCAATAATTATCGCTATTTTATCGATCGCAATTATTGTGATTTCATTGCTTATGTCACCAGACAGTAATGGTTTTTCTGGTGCACTTGTTGGTAGTGGTGATTTGGAACTTTTTAAATATTCAAAAGAAAGAGGACTTAAAAAGGTTTTAAAATACTCAATGTTATTTGGTGGAATAGCATTGCTAATTTTAGCTATTATTTTAAGGATTATTGTATAA", "aa": "MAVIFVSIIIAILSIAIIVISLLMSPDSNGFSGALVGSGDLELFKYSKERGLKKVLKYSMLFGGIALLILAIILRIIV", "aa_hexdigest": "9aa2fc4addf0534ccd25b6c256a57b1c", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32FE4", "ncbi_nrp_id": "WP_013354940.1", "uniref100_id": "UniRef100_C4XF56", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FE4", "RefSeq:WP_013354940.1", "UniRef:UniRef100_C4XF56" ] }, "ips": { "uniref100_id": "UniRef100_C4XF56", "uniref90_id": "UniRef90_C4XF56" }, "psc": { "uniref90_id": "UniRef90_C4XF56", "gene": "secG", "product": "preprotein translocase subunit SecG", "uniref50_id": "UniRef50_A0A4Y6I792", "go_ids": [ "GO:0015136", "GO:0015739", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_A0A4Y6I792", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4Y6I792" ], "product": "Protein-export membrane protein SecG" }, "genes": [ "secG" ], "id": "OAIKOHHJFB_1040", "locus": "GCA000209735_01022" }, { "type": "cds", "sequence": "contig_1", "start": 568589, "stop": 570772, "strand": "+", "frame": 2, "gene": "rnr", "product": "ribonuclease R", "db_xrefs": [ "EC:3.1.13.1", "GO:0003723", "GO:0004518", "KEGG:K12573", "RefSeq:WP_015511008.1", "SO:0001217", "UniParc:UPI0001A8C29A", "UniRef:UniRef100_C4XF57", "UniRef:UniRef50_A5IXZ7", "UniRef:UniRef90_C4XF57" ], "nt": "ATGAATCCGCAAGAAATATTAAATTTTTTAAAAAAACATGATAATTCAACTTTCATTGATATTGCAAAAGGCTTAAGAATAAGTTATAAAGATAACAAAAAATTAACCGAATTACTAAATAAATTAATTGATGAAAGAAAATTAATTAAAAATGGCAAACTCTCAACTTATTCATTAATAGAAAAAATTAAAATCGTCCAAGGAAAAATTCGATTTGCTTCAGAAGGCAAGTATGCTTTTGTAGAAGAAGAAAATGATAATCCTGAAGCTGAAAAAATAAGTTATTTTGTACCTGGTATTCATCTTAATAGAGCATTAGATGGAGACTTTGTTGAAATAACAGTTTTAAAATACTTTCATTCTTTAGATGATAAAACTTTTGCAATTGTTGAAAAAGTTTTAAGCAGAAACAGTGATAAATTCATTGGTATTTTAGGTATGCATGAAGGTTTTCTTTCATTTAAACCACTTAAAAATGACTACAAAAAAATTAATTTTCATATTAAAGAATTGGTTAAAGAAGCTAGACTTCATGATGTTGTTTTAGGTCAAATGGTTTCATTTAAAAATAATATTATTGAGCTTAACATTATCAAAAAAATAGCTAACAGTAATGATCCAATGGCTTATGTTAAAGCCTTAACAGTTATTAGAAATAAACCTGAAGATTTCCCACAAGAAGTTAAAGATTACTTAGTTAAAATTCCAAATAGTATTGCAGATGAAAAACTTAATAATAGACTTGACTTGAGAAAAGAATTAATTGTTACTATTGATGGTAATGACACAAAAGACTTTGATGATGCAATTAATGTTAAAAAGAATGCTGATGGAACTTATGAATTAGGCGTTCATATTGCTGATGTTAGTTATTATGTCAAAGAAGACAGCTCTCTTGATGTTGAAGCATTAAAAAGAGGAACCTCAAGATATTTAGTTAGTGGCGTTATTCCAATGTTGCCTCATAAATTATCAAATGGTATTTGCTCACTTAATCCTAATGAAGATCGCTTTACTCTTAGTGCAATAATGACAATTGATAAAAATGGTGATACTATTGATGCTAAATTTTATCAGTCAATAATTCAATCAAAATATCGTCTCACATATGATAGGGTTAATGAACTTTATGATAAAAATCAAAAATTTGATGACCCGAAATTAAATCGAATGCTCTATGAAGCAGCTGAATTGGCAAAAATAATTAGAAATTATAAAGTCAAAGAAGGCTACATTGATTTTGAAATTGAAGAACCCGAAATTGTCTTAGATGATAAAGGTAAAACAATTGATGTTGTAGTTAAGCCAACTGGTTTTAGTGAAAATCTTATTGAAGATTTCATGGTTAGAACTAATGAAGAAGTAGCTAAATTTTTAGCTAAAAGAAAAATACCTTTTATTTATCGTGTTCATGAAAGCCCTGATAGTGACAAAATTGATTCATTTTTGAGTGTACTTAATGTTTTAGGTATTAAAGTAAACATTGATCGAAATGATATTAATCCAAAAAGTTTTCAAAAAATAATCAACAAAATTAAAGAAACAAGAAATGATGACTTTTTAAAAACAGTTTTCTTAAGAACAATGCAAAAAGCTAAATATTCTCCAAATAATATTGGCCACTTTGGTTTAGCAAGTGAACACTATTGTCACTTTACTAGTCCAATTCGACGTTATCCAGATTTAGTTGTTCATAGGATTTTAAGAGAAATTGTTTTTAACAATGATAAAAGTAAAATTACTCACTACAAAAACATTTTAAGTAATGTTGCAGAATTAAATTCAAATAGTGAACAAGAAGCATTACAACTTGAACGTGATACAAATGATTTAAAATATGCTGAGTTTTTCAAAAACAAAATTGGTCAAACAATGAAAGCTCAAATTATTAGTATTTTACGCTTTGGTATGTTTGTCCAATTTGACTGCAAAATAGAGGCTTTAGTTCATATTTCAACATTATGTGATGGTGATTATGAAGTTAATGAAAACTTTACTGAATTAAGAAGTAAACAACACACTTTTAAACTTGGAGACCAAGTAGAAGTGGTTATTGCTGGAGCTGATGAAACTAACGGTAAAATTGATGCAGTGCTTGCAAAATGCTATGGTTCATACTTAGAAGAAATCAAGAAAAAACTTAATTCAAACTCAAATCGCAAACATAAAAAATCAGGTAAATAA", "aa": "MNPQEILNFLKKHDNSTFIDIAKGLRISYKDNKKLTELLNKLIDERKLIKNGKLSTYSLIEKIKIVQGKIRFASEGKYAFVEEENDNPEAEKISYFVPGIHLNRALDGDFVEITVLKYFHSLDDKTFAIVEKVLSRNSDKFIGILGMHEGFLSFKPLKNDYKKINFHIKELVKEARLHDVVLGQMVSFKNNIIELNIIKKIANSNDPMAYVKALTVIRNKPEDFPQEVKDYLVKIPNSIADEKLNNRLDLRKELIVTIDGNDTKDFDDAINVKKNADGTYELGVHIADVSYYVKEDSSLDVEALKRGTSRYLVSGVIPMLPHKLSNGICSLNPNEDRFTLSAIMTIDKNGDTIDAKFYQSIIQSKYRLTYDRVNELYDKNQKFDDPKLNRMLYEAAELAKIIRNYKVKEGYIDFEIEEPEIVLDDKGKTIDVVVKPTGFSENLIEDFMVRTNEEVAKFLAKRKIPFIYRVHESPDSDKIDSFLSVLNVLGIKVNIDRNDINPKSFQKIINKIKETRNDDFLKTVFLRTMQKAKYSPNNIGHFGLASEHYCHFTSPIRRYPDLVVHRILREIVFNNDKSKITHYKNILSNVAELNSNSEQEALQLERDTNDLKYAEFFKNKIGQTMKAQIISILRFGMFVQFDCKIEALVHISTLCDGDYEVNENFTELRSKQHTFKLGDQVEVVIAGADETNGKIDAVLAKCYGSYLEEIKKKLNSNSNRKHKKSGK", "aa_hexdigest": "68163b68384c38b97a678bf4f6ed4969", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "ups": { "uniparc_id": "UPI0001A8C29A", "ncbi_nrp_id": "WP_015511008.1", "uniref100_id": "UniRef100_C4XF57", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C29A", "RefSeq:WP_015511008.1", "UniRef:UniRef100_C4XF57" ] }, "ips": { "uniref100_id": "UniRef100_C4XF57", "uniref90_id": "UniRef90_C4XF57" }, "psc": { "uniref90_id": "UniRef90_C4XF57", "gene": "rnr", "product": "ribonuclease R", "ec_ids": [ "3.1.13.1" ], "uniref50_id": "UniRef50_A5IXZ7", "kegg_orthology_id": "K12573", "go_ids": [ "GO:0003723", "GO:0004518" ] }, "pscc": { "uniref50_id": "UniRef50_A5IXZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IXZ7" ], "product": "Ribonuclease R" }, "genes": [ "rnr" ], "id": "OAIKOHHJFB_1041", "locus": "GCA000209735_01023" }, { "type": "cds", "sequence": "contig_1", "start": 570775, "stop": 571443, "strand": "+", "frame": 1, "gene": null, "product": "Methyltransferase small domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXG4", "UniRef:UniRef90_C4XF58" ], "nt": "ATGAATAAAAAACAAAAATTAGTTAAAAATTCTTTAGGTTTTGATTCTAATTTATATGTTGTGCAAGACAAAGATATGTTCAACTATTCAGTTGACACTATTATGCTTGGCAACTTTGTTTTTTTAAATAATAAAATTAAAAACCTATTAGAAATTGGAGCAAACAATGGAGCTCTCAGTATTTTTATAGCTGAAAGAAATCCAAATTTAAAAATAGATGCAGTTGAAATCCAAACTAAAGCTTGTAAGTTAGCTCAAGACAATATTGAACTTAACAACAAGCAAAATCAAATCAAAATAATTAATCAAGATTTCAATGAATTTTATTTAGAAAAAATTAAAAATGCAGCTAGTAAATATGACTCAATAGTTTGCAATCCTCCTTTTTATACAATGCAAACTAATAAAGTTAGCAAAAATATTAGTGAAGAATTACTAATTGCAACACATGAGTATAAAATTAATTTAGAACAAATTATTTTAGGTTCTTCAAAATTAATTGAGCAAAAAGGCTACTTAACTTTAGTGATTCCAGTTGAACGCTTAGTTGATTGCTTTTGCTTAATGAGAAAATATAATTTTGAGCCTAAAAGAGTTCAATTCATTATTCCAAGAATGCAAGATAAACCTAAATTAGTTTTAGTTGAAGGCAGATATCAAGCTGGTTGA", "aa": "MNKKQKLVKNSLGFDSNLYVVQDKDMFNYSVDTIMLGNFVFLNNKIKNLLEIGANNGALSIFIAERNPNLKIDAVEIQTKACKLAQDNIELNNKQNQIKIINQDFNEFYLEKIKNAASKYDSIVCNPPFYTMQTNKVSKNISEELLIATHEYKINLEQIILGSSKLIEQKGYLTLVIPVERLVDCFCLMRKYNFEPKRVQFIIPRMQDKPKLVLVEGRYQAG", "aa_hexdigest": "bc2ae9df8dc10fd8e62d00e2fbd2b347", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_C4XF58", "query_cov": 1.0, "subject_cov": 0.844106463878327, "identity": 1.0, "score": 432.0, "evalue": 1.84e-152, "valid": true, "product": "Methyltransferase small domain-containing protein", "uniref50_id": "UniRef50_A0A448ZXG4" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXG4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXG4" ], "product": "DNA methylase" }, "genes": [], "id": "OAIKOHHJFB_1042", "locus": "GCA000209735_01024" }, { "type": "cds", "sequence": "contig_1", "start": 571563, "stop": 571658, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACAAAAGACTTTTTATATAACTACTCCTATTTATTATGCTTCAGGTCCGCTTCATATCGGACATCTTTATTGTACAATTATGGCTTGA", "aa": "MKKQKTFYITTPIYYASGPLHIGHLYCTIMA", "aa_hexdigest": "47b9a5c2cc8114a57fd64ec4c252bdb6", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3591.2688000000007, "isoelectric_point": 9.361470222473145 }, "id": "OAIKOHHJFB_1043", "locus": "GCA000209735_01025" }, { "type": "cds", "sequence": "contig_1", "start": 572487, "stop": 572882, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCCAAATCTTTAAATAATGTTATAGCCCCAGATTATTTATTTGACAAGTATCATCATGAGATGATTAAATACTATTTTGCAACTCAAATAATATTTGGAGAAGATGGTAATTTTAGTGAAGAAAAATTAAAAGAAGTTGTTAATGCAGATTTAATAAATAATTATGGTAATTTAGTTTCAAGAACACTAAAAATGATTCACAATTCTTTCCCAAAAGGTCTGCTTTATAGTGTTAATAGTAAAAAAATACATAAAGACATTGAAAAAGAAATTAAGAATTTCAGTGCTCAATTTCAAAAACATATGGATGCTTTTGAAATTGATAAAGGATTTAAAAAAGCAATTGAATTATCAGCTAAATTAAATAAATATATTGATGAAACAACACCATGA", "aa": "MSKSLNNVIAPDYLFDKYHHEMIKYYFATQIIFGEDGNFSEEKLKEVVNADLINNYGNLVSRTLKMIHNSFPKGLLYSVNSKKIHKDIEKEIKNFSAQFQKHMDAFEIDKGFKKAIELSAKLNKYIDETTP", "aa_hexdigest": "4486b9ec4ed3157aafe97fb5be130175", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15257.295799999984, "isoelectric_point": 7.874249076843262 }, "id": "OAIKOHHJFB_1044", "locus": "GCA000209735_01026" }, { "type": "cds", "sequence": "contig_1", "start": 572907, "stop": 573107, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAACCAATTTTAGTAAGATTATTAAATGGAATTTATGCTGTTAGTTGTGCATTACAAATAGTATTGCCTACAAAAATAAAAGAAGTTGCAAGTGTTTTAAAAGTTAAAAAATTTGACTTTGAAAAAATTAATGATTTCAATAAATTTGATAAAGTTATTTGTGATGAGAAATATTTGTTATTTAATAGATTAAAATAA", "aa": "MEPILVRLLNGIYAVSCALQIVLPTKIKEVASVLKVKKFDFEKINDFNKFDKVICDEKYLLFNRLK", "aa_hexdigest": "609aac8a8f3aae22747e0ef7c4f0caa4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7704.206999999998, "isoelectric_point": 9.23040599822998 }, "id": "OAIKOHHJFB_1045", "locus": "GCA000209735_01027" }, { "type": "cds", "sequence": "contig_1", "start": 573594, "stop": 573851, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00044.30" ], "nt": "ATGAAAAAAGTTGCAATCAACGGTTTCGGTCGTATTGGACGTTTAACACTTCGTCACTTACTTGAAACCCAAAATAAAGAAGTTCAAGTTGTTGCAGTTAACGACTTAACAGATCCAAAAATGTTAGCACATTTACTTAAATACGATACAGCTTTTGGCCCATTGGAAGCTAAGGTTGAAGTTAAAGAAAATGCTATTAAAGTTAATGGAAAAGAAATTAAAGTATTCTGCGAAAAAGATCCAGCTAACTTACCATGA", "aa": "MKKVAINGFGRIGRLTLRHLLETQNKEVQVVAVNDLTDPKMLAHLLKYDTAFGPLEAKVEVKENAIKVNGKEIKVFCEKDPANLP", "aa_hexdigest": "c073492bc640cadb5a1b7ec5609d0565", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0024BE2B32", "query_cov": 0.9764705882352941, "subject_cov": 0.8469387755102041, "identity": 0.6509999999999999, "score": 104.0, "evalue": 1.29e-27, "valid": false, "product": "glyceraldehyde 3-phosphate dehydrogenase NAD-binding domain-containing protein", "uniref50_id": "UniRef50_A0A2M8L0B5" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00044.30", "name": "Gp_dh_N", "length": 83, "aa_cov": 0.9764705882352941, "hmm_cov": 0.9759036144578314, "evalue": 6.182316685200041e-31, "score": 102.83222198486328, "start": 3, "stop": 85 } ], "seq_stats": { "molecular_weight": 9498.060999999992, "isoelectric_point": 9.131253623962404 }, "id": "OAIKOHHJFB_1046", "locus": "GCA000209735_01028" }, { "type": "cds", "sequence": "contig_1", "start": 574116, "stop": 574532, "strand": "+", "frame": 3, "gene": null, "product": "Glyceraldehyde-3-phosphate dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PH09" ], "nt": "ATGACAACAGTTCACGCTTACACAGGTGACCAAATGTTACAAGATGGTCCACACCGTAAAGGTGACTTAAGACGTGCTCGTGCTGCTGCTCAAAACATTGTTCCTTCAACAACAGGTGCTGCAAAAGCTATTGGTTTAGTTGTTCCCGAAGCTGATGGATTATTAGATGGTTCAGCATTGCGTGTTCCTACAATTACAGGTTCATTCGTTGACTTAACAGTTCAATTGAAAAAACAACCTACAGTTGAAGAAATTAATGCTGCATTCAAAAAAGCAGAAAACGAAACAATGAAATATGTAGTTGACCCTATTGTTTCTTCAGACGTTATTAACTCACACTATGGTTCATTATTCGATTCAACTTTAACAAAAGTTAAAGAAGGAAATGGTCAAAGACTTTACAAAATCTTTGCATGA", "aa": "MTTVHAYTGDQMLQDGPHRKGDLRRARAAAQNIVPSTTGAAKAIGLVVPEADGLLDGSALRVPTITGSFVDLTVQLKKQPTVEEINAAFKKAENETMKYVVDPIVSSDVINSHYGSLFDSTLTKVKEGNGQRLYKIFA", "aa_hexdigest": "6b35d1e376449254ce947b31e6efdcc8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D4XW52", "query_cov": 1.0, "subject_cov": 0.8679245283018868, "identity": 0.703, "score": 192.0, "evalue": 1.95e-60, "valid": false, "product": "Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain protein", "uniref50_id": "UniRef50_A0A3B0PH09" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PH09", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PH09" ], "product": "Glyceraldehyde-3-phosphate dehydrogenase" }, "genes": [], "id": "OAIKOHHJFB_1047", "locus": "GCA000209735_01029" }, { "type": "cds", "sequence": "contig_1", "start": 574701, "stop": 575402, "strand": "+", "frame": 3, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "RefSeq:WP_013354934.1", "SO:0001217", "UniParc:UPI0001E32FDF", "UniRef:UniRef100_C4XF62", "UniRef:UniRef50_C4XF62", "UniRef:UniRef90_C4XF62" ], "nt": "ATGAAGAAAAACTTATTAAAGTTAACAGGAATTATTGCTCCAGTTATTACAGCTATACCATTTGTTGCTTCATCATGTGGTAGTAAAGATGAAGCAACTTTAGCTGATCTTTTAAAGGAAAAATTGACAAAGGATGCAGATGCTTCTAAATATTCTGCACTAATTACTGACAAGGAAAAATTAAAACAATTCAAGGAATTAGTAGAAGAATTTAAAAAAGAAGGAGTAGATTTAACTGCTAAAGGTAATCCTTCTGATGTAGATAATGCAATTAAAACTATTAAAGAAAAAGCGCCATTAGTTTTAGCTAGAACAATTAAAGCAATGGTTGAAAACGGAAGTGCTTTTATAATTAAAGAAGATGGGATTGCTAGTGCTAAACAAAAACTAGAAAATGGTGATATATTTGCTCAAGTTTATCCTGCACAACATAAAATCGGAAGATCTGCTTATACAATTATTGAATTTACTGAAAAATTTAAGGCATTTTATTTAACTTCAAAAGTATTCGAAGTTGCTAGAATTTCAACATTTTATGAAAATAAATATGGTGGTAAAACTCAACCTCATGCAAACGGAGTAAACTTAGAATTTGTTAAAGGCAAGTTAAATATTAAATTTGTGATTTGTGATTTGCTAAAAGGCGACCAAACTGGTTCAGTTGTTTTAGTAAATAACTTATTATTTGCAGTTGAAAAATAA", "aa": "MKKNLLKLTGIIAPVITAIPFVASSCGSKDEATLADLLKEKLTKDADASKYSALITDKEKLKQFKELVEEFKKEGVDLTAKGNPSDVDNAIKTIKEKAPLVLARTIKAMVENGSAFIIKEDGIASAKQKLENGDIFAQVYPAQHKIGRSAYTIIEFTEKFKAFYLTSKVFEVARISTFYENKYGGKTQPHANGVNLEFVKGKLNIKFVICDLLKGDQTGSVVLVNNLLFAVEK", "aa_hexdigest": "4ad2567d9b653639e138017a546a76b7", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32FDF", "ncbi_nrp_id": "WP_013354934.1", "uniref100_id": "UniRef100_C4XF62", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FDF", "RefSeq:WP_013354934.1", "UniRef:UniRef100_C4XF62" ] }, "ips": { "uniref100_id": "UniRef100_C4XF62", "uniref90_id": "UniRef90_C4XF62" }, "psc": { "uniref90_id": "UniRef90_C4XF62", "product": "Lipoprotein", "uniref50_id": "UniRef50_C4XF62" }, "pscc": { "uniref50_id": "UniRef50_C4XF62", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF62" ], "product": "Lipoprotein" }, "genes": [], "id": "OAIKOHHJFB_1048", "locus": "GCA000209735_01030" }, { "type": "cds", "sequence": "contig_1", "start": 575716, "stop": 575919, "strand": "+", "frame": 1, "gene": null, "product": "DUF4044 domain-containing protein", "db_xrefs": [ "RefSeq:WP_013527119.1", "SO:0001217", "UniParc:UPI0001F598D7", "UniRef:UniRef100_C4XF63", "UniRef:UniRef50_A0A3P8MEH3", "UniRef:UniRef90_C4XF63" ], "nt": "ATGGTTTTAATTATTATCTTAGCTTCTATTAAGAACTTCGATGCTGCAGAATCTTTAAAAACAACAAATGCTAAATACGTAGCCATTGTTGCCTTATTTTTCTTTATATCTTTATGTGCTGCAATTTTTGGAACAGTTATTTTATCAATCTTAGATAAAAAAGCTGTTACAAAAAAAGCACAACAAAATCAAGGAGGTAAATAA", "aa": "MVLIIILASIKNFDAAESLKTTNAKYVAIVALFFFISLCAAIFGTVILSILDKKAVTKKAQQNQGGK", "aa_hexdigest": "03fee40d101b04b203a052afb30f3c4b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F598D7", "ncbi_nrp_id": "WP_013527119.1", "uniref100_id": "UniRef100_C4XF63", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F598D7", "RefSeq:WP_013527119.1", "UniRef:UniRef100_C4XF63" ] }, "ips": { "uniref100_id": "UniRef100_C4XF63", "uniref90_id": "UniRef90_C4XF63" }, "psc": { "uniref90_id": "UniRef90_C4XF63", "uniref50_id": "UniRef50_A0A3P8MEH3" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MEH3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEH3" ], "product": "DUF4044 domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_1049", "locus": "GCA000209735_01031" }, { "type": "cds", "sequence": "contig_1", "start": 575919, "stop": 576596, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGGTACAAAAACACAATCATCAAGACCTTCATTTGGAAAAAGACTATTAGAAAAACTTGGACGCATTGGTCGGGTATTGATGTTCCCTATTGCAGTTCTTCCTATTGCTGCTATTCTTCTTAGAGTTGGAGCTGATTTACCAACAGTTAAAGCTGGACCTGATATTTCACAAGTCGAAGCTAACTTTGTTAACTTTGTTCAACAAATGATAACAGCAGGCGGATCAGTTGTATTTGACAACTTGTACATTTTATTTGCCGTCGGAATAGGATTTGGTTTTACTAAAGATAACCGTGGTGAAGCTGCTTTTGCTGCTTTAATTAGTATTCTATTACTTTCACTTTTATTAAAAAGTGGTGGATATGTAGATAAAATCTACAGCACATTTAAACTCCCTGGAGCCGAATTTAATGAAGATGGTAAAAAACTTGAAGCTTTTATAGCTGAAGGTAAACCTGCTTCAGAATTTGTTATAGCTAATGGTTTTGCAGGTGTCTTTGGTGGCAAATACAATGCAATAATGTCTGGCAATGTACTTAATGGTATTATTGTTGGATTTATTGTTGCAGCTATTTATAACCATACAAATGATGTTCAATTACCTAAAGTTCTTGGATTCTTTTCGGGTAGAAGATTAGTTCCAGCTCTTGCAATTCTTTCAACTCTTATTTGA", "aa": "MTGTKTQSSRPSFGKRLLEKLGRIGRVLMFPIAVLPIAAILLRVGADLPTVKAGPDISQVEANFVNFVQQMITAGGSVVFDNLYILFAVGIGFGFTKDNRGEAAFAALISILLLSLLLKSGGYVDKIYSTFKLPGAEFNEDGKKLEAFIAEGKPASEFVIANGFAGVFGGKYNAIMSGNVLNGIIVGFIVAAIYNHTNDVQLPKVLGFFSGRRLVPALAILSTLI", "aa_hexdigest": "eab4b7a4d83c5d0a21045785c86786e9", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23917.7978, "isoelectric_point": 9.58169422149658 }, "id": "OAIKOHHJFB_1050", "locus": "GCA000209735_01032" }, { "type": "cds", "sequence": "contig_1", "start": 576641, "stop": 576865, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTCCTTTTGCTTTACCACTTAAGAAAATAAAGATATCACCAAATACAGGTGTACCATCAGGAGTTACATGTTCTCCTAATTGGAATCAGAATAAGTTGTTTGGAATGTGGTGTAATCCAAATGGAATTAATAGACGATTAATAACGCCATATACACCCATAATAGCAGCTGAAGCTCAACGACCTTTAGCTGCACCTGATAACACTTTTGAAAATGCATAA", "aa": "MFPFALPLKKIKISPNTGVPSGVTCSPNWNQNKLFGMWCNPNGINRRLITPYTPIIAAEAQRPLAAPDNTFENA", "aa_hexdigest": "08b603faac097bd854914613f42c5a16", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8155.374299999995, "isoelectric_point": 9.589817237854003 }, "id": "OAIKOHHJFB_1051", "locus": "GCA000209735_01033" }, { "type": "cds", "sequence": "contig_1", "start": 576903, "stop": 577247, "strand": "+", "frame": 3, "gene": null, "product": "PTS transporter subunit EIIC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035A088D1" ], "nt": "ATGTTTGGTCTTCCTGCTCTAGCATTTGCTTTCTACATGACTGCAGATGATAAAGTGCAAAAGAGAAGAGTAATGGCTATCTTTGGAGCAGCCGCAGTAGTATCAATTCTTTCAGGTATTACTGAACCTATCGAATTTGCATTTATGTTTGTTAGTCCATTATTATATGGTTTCCACTGCATTCTAACTGGTATATTTGCATTTATAACTGGTGCATTTGGAATTCAAATTGGATTTGGGTTCTCAGCTGGTTTAATGGATTACTTATTATCAATTCCTAAATCATTAGCAATTATTAAAGCTAACAAAACAGGTATTGATGCTGTATTAGCTAACCCTGGTTGA", "aa": "MFGLPALAFAFYMTADDKVQKRRVMAIFGAAAVVSILSGITEPIEFAFMFVSPLLYGFHCILTGIFAFITGAFGIQIGFGFSAGLMDYLLSIPKSLAIIKANKTGIDAVLANPG", "aa_hexdigest": "d33d0bc54d09b9fc48e069f20f333793", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_UPI0016429E17", "query_cov": 0.8070175438596491, "subject_cov": 0.9019607843137255, "identity": 0.5329999999999999, "score": 91.3, "evalue": 9.15e-22, "valid": false, "product": "PTS transporter subunit EIIC", "uniref50_id": "UniRef50_UPI0035A088D1" }, "pscc": { "uniref50_id": "UniRef50_UPI0035A088D1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035A088D1" ], "product": "PTS transporter subunit EIIC" }, "genes": [], "id": "OAIKOHHJFB_1052", "locus": "GCA000209735_01034" }, { "type": "cds", "sequence": "contig_1", "start": 577350, "stop": 577673, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00367.25" ], "nt": "TTGCCTGATCCAAATGCTTTAACAGAAGTTAAATCAACTTCAAGTGCTTCTGGTGAAGAAACTCCAACAGATTCGAAATTAAATAACCTAAGTCTAAAAGCAAAAGCTATTGTTGAAGCTGTTGGTGGTTACGAAAACATTACAACTTTTGCAAACTGTGCAACAAGACTTCGTTATGAAGTTAAAGACTCAGATATTGTTGATGAAGCAAAATTAAAACTTGCGGGATGCAGTGGTTTGATTAGATTAGGAAAGAATTCAATTCAAATTATTGTAGGTGTTGAAGCTGAAAACATTAACAATGAAATTGAATCAAATAAATAA", "aa": "MPDPNALTEVKSTSSASGEETPTDSKLNNLSLKAKAIVEAVGGYENITTFANCATRLRYEVKDSDIVDEAKLKLAGCSGLIRLGKNSIQIIVGVEAENINNEIESNK", "aa_hexdigest": "5af06d39b2de6278ce5dade57c7f8897", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00367.25", "name": "PTS_EIIB", "length": 34, "aa_cov": 0.3177570093457944, "hmm_cov": 1.0, "evalue": 1.5816729846709398e-16, "score": 55.58298110961914, "start": 35, "stop": 68 } ], "seq_stats": { "molecular_weight": 11426.709099999993, "isoelectric_point": 4.8286657333374015 }, "id": "OAIKOHHJFB_1053", "locus": "GCA000209735_01035" }, { "type": "cds", "sequence": "contig_1", "start": 577711, "stop": 578397, "strand": "-", "frame": 2, "gene": null, "product": "Phage-Barnase-EndoU-ColicinE5/D-RelE like nuclease 4 domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354931.1", "SO:0001217", "UniParc:UPI0001E32FDC", "UniRef:UniRef100_C4XF65", "UniRef:UniRef50_A0A3P8MDF4", "UniRef:UniRef90_C4XF65" ], "nt": "ATGAATAATCAAAATTTTAAGAATTTATTAACTAAATATATTGAATTCTTTTATAGGTATAAAAGCATTTCATTATTAAAAAAGGCAAATAATGAATCACAATTTGATGTTAAATTAAGATTAGTTTTAACACAAAAATTTTTACTGCATTCACTAGGTTTGCAATACAACAAAAAGTTATTTAGAAAATATAAGAACAATAATTTTGAACTTTATAGAGATATAAAAAATGATCAACTAAATTATGATGAGTTAATTAAAGACATAAGCAAAAACAAAAAAGATTTAATTGACAAAAAATTAAACGCTCTTGAGATTTTTGTCAATATTATAAAAAATATTAATCAAGAAAAACTATTTACTTTTGCATTTGAATTTTTTAAATTAAAACCAAAATTTGATATTGATGGAGCTTATAACCATTCACAATTGATTTTAGTTACAAAAAGCAATAAAGAAAATCAAAGATGTATTATTTATGGAATTTTGGGAGATTTATATACCACCAATTCAAAAGATAATAATCATTTTTTAAACGAAAATAAAAATCTTATGAACACAGTTTTTATACCTATATCAATAAAAATCAAAAACAATGAAAATCTTTTAAATTCTCAAAATATACAAAAAATTGATATTGCATTTTTAAACAAAAGAAGAATACAAGAAAAAGAAATAAGAGTATAA", "aa": "MNNQNFKNLLTKYIEFFYRYKSISLLKKANNESQFDVKLRLVLTQKFLLHSLGLQYNKKLFRKYKNNNFELYRDIKNDQLNYDELIKDISKNKKDLIDKKLNALEIFVNIIKNINQEKLFTFAFEFFKLKPKFDIDGAYNHSQLILVTKSNKENQRCIIYGILGDLYTTNSKDNNHFLNENKNLMNTVFIPISIKIKNNENLLNSQNIQKIDIAFLNKRRIQEKEIRV", "aa_hexdigest": "7616135b0e1370eea83caffd82d75333", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32FDC", "ncbi_nrp_id": "WP_013354931.1", "uniref100_id": "UniRef100_C4XF65", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FDC", "RefSeq:WP_013354931.1", "UniRef:UniRef100_C4XF65" ] }, "ips": { "uniref100_id": "UniRef100_C4XF65", "uniref90_id": "UniRef90_C4XF65" }, "psc": { "uniref90_id": "UniRef90_C4XF65", "uniref50_id": "UniRef50_A0A3P8MDF4" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MDF4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MDF4" ], "product": "Phage-Barnase-EndoU-ColicinE5/D-RelE like nuclease 4 domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_1054", "locus": "GCA000209735_01036" }, { "type": "cds", "sequence": "contig_1", "start": 578468, "stop": 578671, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAATGACACAATTGCAGCAATATCTTCAGGTGGTAAGATTAATCAAGCCATATCAATAATCAGAGTTAGTGGTCAAGATAGTATTGAAATAGTTTCTAAAATTTTTAAAGGAAAAATAGGAACAAATCAAACAATAACTTATGGCCATATTTTTGATAATAAAACAAAAGAAATTATTGATGAAGTTTTAATTGCTTGA", "aa": "MINDTIAAISSGGKINQAISIIRVSGQDSIEIVSKIFKGKIGTNQTITYGHIFDNKTKEIIDEVLIA", "aa_hexdigest": "16c3ea807b913ca42a1c14ac7cfdd624", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7233.255399999998, "isoelectric_point": 6.533664894104004 }, "id": "OAIKOHHJFB_1055", "locus": "GCA000209735_01037" }, { "type": "cds", "sequence": "contig_1", "start": 578783, "stop": 579727, "strand": "+", "frame": 2, "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "db_xrefs": [ "GO:0003924", "GO:0005525", "GO:0006400", "SO:0001217", "UniRef:UniRef50_A5IXJ1", "UniRef:UniRef90_C4XF66" ], "nt": "ATGGCACTGCCAGGTGAATTTAGTCGACGTTCATTTTTGAATGGTAAAATGGACTTAATTAAAGCTGAAGCAATTAATGATTTAATACATGCCAAAACATTAAAACAAAGTGAATTAGCCATTAAGAAATTTGATGGCAAAACTTCAAACATGATTAAATCATTTATTGACGAATTAGCCTATTTAATAGGTCAAATGGAAATAAATATAGATTATCCAGAGTATGAAGATTTTGAAAATGTTTTGACAAAAGATTTAATTTCTAGATTGCAAAAATTGCAAGACAAATTGAGCTTAATTATCAAAGAAAGTGAAAACTCAAGATTAATTTTTGATGGCATTAAAGTTGCTATTCTTGGTAAGCCAAATGTTGGAAAAAGTTCAATATTAAATTGCTTAATAAATGAAGATAAAGCAATAGTTACAGATCAGGCTGGAACAACTCGTGATCTTGTTGAAGCTTCATATCAAATTGATGGTTTATTATTCAAATTAGTTGATACCGCTGGAATTAGAAAAACTAATCGAAAAATTGAAAAAATAGGTATTGAAAAATCTTTTGAACAAATTGAAAAATGTGATGTTGTAATTCATGTTAATGAAGCTAATACTTTAGAAAATGATTACGATAAAAAAGTTGAAAAATTAGCTAAACAATTTAACAAACCATTAATTAAAGTAATTAATAAAGTTGATTTATTAAAAAATAATAAAAAGAAAAAGAATGTTATTTATGTCAGTGCTAAAAATAAAAACATTGATGATTTAAAGGAAGCATTAGTTAATATTTTTTCAAAGAATGAAATTAACAATGAAGAATACGTTGTTAATGCAAGACAACTAGCATTGGTTAAAAAGGCTCATTCAAGTATTTCTGATGCAATTGAATCAATAAATAATGGCATTGATGTTGATGTAGTAATTATTGATGTAAGACAAGCTTGA", "aa": "MALPGEFSRRSFLNGKMDLIKAEAINDLIHAKTLKQSELAIKKFDGKTSNMIKSFIDELAYLIGQMEINIDYPEYEDFENVLTKDLISRLQKLQDKLSLIIKESENSRLIFDGIKVAILGKPNVGKSSILNCLINEDKAIVTDQAGTTRDLVEASYQIDGLLFKLVDTAGIRKTNRKIEKIGIEKSFEQIEKCDVVIHVNEANTLENDYDKKVEKLAKQFNKPLIKVINKVDLLKNNKKKKNVIYVSAKNKNIDDLKEALVNIFSKNEINNEEYVVNARQLALVKKAHSSISDAIESINNGIDVDVVIIDVRQA", "aa_hexdigest": "d6cd6218eb82f148bc43dd1d7491eff9", "start_type": "ATG", "rbs_motif": null, "genes": [ "mnmE" ], "truncated": "both", "pseudogene": { "start": 578483, "stop": 579811, "observations": { "mutation": [ 578669, 579725 ], "stop": [ 578669, 579725 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF66", "query_cov": 1.0, "subject_cov": 0.6977777777777778, "identity": 1.0, "score": 587.0, "evalue": 3.83e-209, "gene_start": 1, "gene_end": 314, "reference_start": 109, "reference_end": 422, "reference_sequence": "MYIMINDTIAAISSGGKINQAISIIRVSGQDSIEIVSKIFKGKIGTNQTITYGHIFDNKTKEIIDEVLIAWFIGNKNFTGENTVEINCHGGVLVTNRILELLLANGARMALPGEFSRRSFLNGKMDLIKAEAINDLIHAKTLKQSELAIKKFDGKTSNMIKSFIDELAYLIGQMEINIDYPEYEDFENVLTKDLISRLQKLQDKLSLIIKESENSRLIFDGIKVAILGKPNVGKSSILNCLINEDKAIVTDQAGTTRDLVEASYQIDGLLFKLVDTAGIRKTNRKIEKIGIEKSFEQIEKCDVVIHVNEANTLENDYDKKVEKLAKQFNKPLIKVINKVDLLKNNKKKKNVIYVSAKNKNIDDLKEALVNIFSKNEINNEEYVVNARQLALVKKAHSSISDAIESINNGIDVDVVIIDVRQAWADLVDISGRADNELLLDEMFKNFCLGK" }, "paralog": false, "identity": 0.995475113122172, "score": 842.0, "evalue": 2.66e-312, "description": "Internal stop codon at 578669, 579725. Nonsense mutation around 578669, 579725.", "psc": { "uniref90_id": "UniRef90_C4XF66", "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "uniref50_id": "UniRef50_A5IXJ1", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0006400" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF66", "UniRef:UniRef50_A5IXJ1", "GO:0003924", "GO:0005525", "GO:0006400" ] }, "pscc": { "uniref50_id": "UniRef50_A5IXJ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IXJ1" ], "product": "tRNA modification GTPase MnmE" } }, "id": "OAIKOHHJFB_1056", "locus": "GCA000209735_01038" }, { "type": "cds", "sequence": "contig_1", "start": 579831, "stop": 580307, "strand": "+", "frame": 3, "gene": "tatD", "product": "TatD DNase family protein", "db_xrefs": [ "EC:3.1.21.-", "KEGG:K03424", "SO:0001217", "UniRef:UniRef50_C4XF67", "UniRef:UniRef90_C4XF67" ], "nt": "ATGTCAATAAAATATATTGATGCTCATACTCATCCATTAAAAGAATACTATGATGACAATTATCATGTAATTGAAAGAGCTCATGCTAAAGGTTTAGTAGCTTTATTAATTACCGGATGTAATGAACAAGAAAATGAAGAAGTTATTAAAATTGCCAAAAACTTTTCATATACTTTTCCTGTTATTGGAATTCATCCAAATGAATGTCATGGAAAGGTTGACGGCGAAATTATTGAAAAGCAATTAGATGATTCTGTTGTTGCAATTGGCGAAATTGGTTTAGATTACTTTTATACACCAGAAAAGAAAGATATTCAAATTGAATCATTGCATGCTCAAATTCAAGTAGCTAAAAAACACAATTTACCAGTAGTAATTCACATGCGTGATGCATATGAAGATTTGTACGAAATTATTAAACAATATTATAAAGATGTTGTTTTTATGATTCATACATATAGTGGTAACTTATATTGA", "aa": "MSIKYIDAHTHPLKEYYDDNYHVIERAHAKGLVALLITGCNEQENEEVIKIAKNFSYTFPVIGIHPNECHGKVDGEIIEKQLDDSVVAIGEIGLDYFYTPEKKDIQIESLHAQIQVAKKHNLPVVIHMRDAYEDLYEIIKQYYKDVVFMIHTYSGNLY", "aa_hexdigest": "ddfea2fffa4234f3aed689ee36d5ef5a", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "tatD" ], "truncated": "3-prime", "pseudogene": { "start": 579831, "stop": 580604, "observations": { "mutation": [ 580305, 580398 ], "stop": [ 580305, 580398 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF67", "query_cov": 1.0, "subject_cov": 0.6147859922178989, "identity": 1.0, "score": 323.0, "evalue": 1.42e-110, "gene_start": 1, "gene_end": 158, "reference_start": 1, "reference_end": 158, "reference_sequence": "MSIKYIDAHTHPLKEYYDDNYHVIERAHAKGLVALLITGCNEQENEEVIKIAKNFSYTFPVIGIHPNECHGKVDGEIIEKQLDDSVVAIGEIGLDYFYTPEKKDIQIESLHAQIQVAKKHNLPVVIHMRDAYEDLYEIIKQYYKDVVFMIHTYSGNLYWAKKFYELGCYFSFSGVATYKNGTETIEVLDWLPIDRILTETDAPFLSPAHKRGELNYPNYVIQTTHFIAGIKKIPIEKFTDQILKNAKDLFKINVSRK" }, "paralog": false, "identity": 0.9922178988326849, "score": 519.0, "evalue": 3.8e-190, "description": "Internal stop codon at 580305, 580398. Nonsense mutation around 580305, 580398.", "psc": { "uniref90_id": "UniRef90_C4XF67", "product": "TatD DNase family protein", "ec_ids": [ "3.1.21.-" ], "uniref50_id": "UniRef50_C4XF67", "kegg_orthology_id": "K03424", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF67", "EC:3.1.21.-", "UniRef:UniRef50_C4XF67", "KEGG:K03424" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF67", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF67" ], "product": "TatD DNase family protein" } }, "id": "OAIKOHHJFB_1057", "locus": "GCA000209735_01039" }, { "type": "cds", "sequence": "contig_1", "start": 580401, "stop": 580604, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01026.27" ], "nt": "TTGCCAATTGATAGAATATTAACTGAAACTGATGCTCCTTTTCTTTCTCCAGCTCACAAAAGAGGTGAATTAAATTATCCAAATTATGTAATTCAAACAACTCACTTTATTGCAGGTATTAAAAAAATACCAATTGAAAAATTTACTGACCAGATTTTAAAAAATGCAAAGGATTTATTTAAGATAAATGTTTCAAGAAAATAA", "aa": "MPIDRILTETDAPFLSPAHKRGELNYPNYVIQTTHFIAGIKKIPIEKFTDQILKNAKDLFKINVSRK", "aa_hexdigest": "6f4369414dd83fa7ff7eea226a90ebd1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01026.27", "name": "TatD_DNase", "length": 61, "aa_cov": 0.9104477611940298, "hmm_cov": 1.0, "evalue": 1.1986137698565326e-16, "score": 56.94118118286133, "start": 1, "stop": 61 } ], "seq_stats": { "molecular_weight": 7738.960099999998, "isoelectric_point": 9.62998104095459 }, "id": "OAIKOHHJFB_1058", "locus": "GCA000209735_01040" }, { "type": "cds", "sequence": "contig_1", "start": 580588, "stop": 581364, "strand": "+", "frame": 1, "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "db_xrefs": [ "EC:2.1.1.182", "GO:0000179", "GO:0006364", "RefSeq:WP_013527117.1", "SO:0001217", "UniParc:UPI0001A8C2A5", "UniRef:UniRef100_C4XF68", "UniRef:UniRef50_N9V0N0", "UniRef:UniRef90_C4XF68" ], "nt": "ATGTTTCAAGAAAATAAATTAAAAGCCAAAAAAAGATATGGTCAAAATTTTTTAAAAGATCAAAATGTTATTAACAAAATAATTGAATTGATTAAACCACAAGGTGAAAAGATTTTAGAAATAGGCCCCGGAAGAGGTGCTTTAACTAAAATTTTAAATCAACAAGCAAAAGAATTTACTGCTTTTGAAATAGATCAAGATATGGTTGATTATTTACAAAAAAATAATATCTTAAATCAAAATCAAATTGTCCAAGGAGATTTTTTAATAGCTGATTTGAAAAAATATAAAAGCTATGTTGTTGTTGGGAATATTCCTTATTACATAACTAGTGATATTATTTTTAAACTATTAGATTATCGACACAATTTTAAAAAAGCTATTCTTATGGTTCAAAATGAAGTGGCTCAAAGATTAGTGGCTAAACCTAATCAAAATGATTACAGCAAACTTTCAATAACAGTTCAATATTGTGCTGATGTTAAAAAAGAATTATTTGTTAAGAAGACTTATTTTGATCCAGTTCCTAAAGTTGATTCTGCTATTGTTTCTATTACTTTTAAAAATGAAGCAAATGATAATTATGAAAATTTAAAAGACTTTTTCAAATTATGTTTTTTAGCTAGAAGAAAAAAATTATCATTTGCTTTAGCTACAAAATATAGTAATGATAAAATTAAAATTGCATATAATAAATTAAATTTAAAAGAATTAACTAGAATTCAAGAATTAGATTTAAAAACTATATTAGCTTTATACGATTGCTTAGAAAATTAG", "aa": "MFQENKLKAKKRYGQNFLKDQNVINKIIELIKPQGEKILEIGPGRGALTKILNQQAKEFTAFEIDQDMVDYLQKNNILNQNQIVQGDFLIADLKKYKSYVVVGNIPYYITSDIIFKLLDYRHNFKKAILMVQNEVAQRLVAKPNQNDYSKLSITVQYCADVKKELFVKKTYFDPVPKVDSAIVSITFKNEANDNYENLKDFFKLCFLARRKKLSFALATKYSNDKIKIAYNKLNLKELTRIQELDLKTILALYDCLEN", "aa_hexdigest": "8ce331cce275527d49d2396d62fefb7c", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "ups": { "uniparc_id": "UPI0001A8C2A5", "ncbi_nrp_id": "WP_013527117.1", "uniref100_id": "UniRef100_C4XF68", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C2A5", "RefSeq:WP_013527117.1", "UniRef:UniRef100_C4XF68" ] }, "ips": { "uniref100_id": "UniRef100_C4XF68", "uniref90_id": "UniRef90_C4XF68" }, "psc": { "uniref90_id": "UniRef90_C4XF68", "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "ec_ids": [ "2.1.1.182" ], "uniref50_id": "UniRef50_N9V0N0", "go_ids": [ "GO:0000179", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_N9V0N0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9V0N0" ], "product": "Ribosomal RNA small subunit methyltransferase A" }, "genes": [ "rsmA" ], "id": "OAIKOHHJFB_1059", "locus": "GCA000209735_01041" }, { "type": "cds", "sequence": "contig_1", "start": 581372, "stop": 581521, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACTTAGATTTAGAATATGAAAAAGAATTAATTGTTGTTTGTAAAAACTATCATGAAGAAGCACAAGCTTATAAAAATGCCAAAAAGTTCATAATTATTTTAGTAACGCTTTGCTATCTTATTGCTTTTGGACTTTTTGCGGTTTGA", "aa": "MDLDLEYEKELIVVCKNYHEEAQAYKNAKKFIIILVTLCYLIAFGLFAV", "aa_hexdigest": "20f9c44fba52834201648f7fe98c8b53", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5727.751199999997, "isoelectric_point": 5.090181922912596 }, "id": "OAIKOHHJFB_1060", "locus": "GCA000209735_01042" }, { "type": "cds", "sequence": "contig_1", "start": 581645, "stop": 581797, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGCCATCAAGTATTATGAAAGTGATCAAAAAGAAAAAGCTATTAAATACTATAAAGCATATTGTACAATTGCTTTTAAACGCAAAAAAGATTACAGTGATAAAAATTCTAATAACAAAAATGATGATGACACAATAATAGTTAAATAA", "aa": "MKAIKYYESDQKEKAIKYYKAYCTIAFKRKKDYSDKNSNNKNDDDTIIVK", "aa_hexdigest": "238be7c57edffd201e402de07e650815", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6018.782800000004, "isoelectric_point": 9.352766990661618 }, "id": "OAIKOHHJFB_1061", "locus": "GCA000209735_01043" }, { "type": "cds", "sequence": "contig_1", "start": 581999, "stop": 582805, "strand": "+", "frame": 2, "gene": null, "product": "Glycerol-3-phosphate dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A168RLR2", "UniRef:UniRef90_C4XF70" ], "nt": "TTGGACTTAAATGAAGCTTTAAAAGAATTTGATTATTTAATTTTAGCTGTACCTTCAAGTGCAATTACTTCTGTGTTAGAACAAATAAAGGAAACAATCAAAAATAAAAAAATAAATGTTATTAATGTTGCAAAAGGTATTGATGAAAAAACAAATGAATTTTTTTCAGAAGTTTTAAACAAACAATTTGCGAATAATTTAAAAAATTATTGTACTTTAATTGGTCCTTCTTTTGCTGTTGAAGTTTTTGAAAATGTTTTAACCATGATTAATGTTGTTGGTCCAAAAACAAGATTTTTAAAAAAAGTTTCTGAATTATTTAATAATGACACTTTTAGACTTGTTGTTAATGAAAATGAACACGGTTCAGAATTGTTTGCTGCTCTAAAAAATGTTCTAGCTATTGGAATTGGAATGATTGATTATTTAAGACCATATCGAAATACAATGGCGGCCTTGCTTTCAATTGGTGTTAAAGAAATTCACATGGTTTACAAGAGAATATTTCCTGAATCAAATGACAATATGGGATTTGAATTAGCAGGAATTGGCGATATCTTTTTAACTTGTTCAAGTACAAAAAGCCGTAACTTTTCTTTTGGTAGACAAGTTGCAGAATTTGGCTTGGAAGCAACTTTAAAAAATAATAAGAAAACTATTGAAGGATATCATGCTGCTAAAATATTAGCTAATATTCTAAAGAAAAATAAAGATCTTAAAATTGTATTTTTAAAAAGCATAATTGATATTTTATATAAAAACAAGAAACCTGAATTACTTTTAGATTTTGTTAAAAAATATAACTAA", "aa": "MDLNEALKEFDYLILAVPSSAITSVLEQIKETIKNKKINVINVAKGIDEKTNEFFSEVLNKQFANNLKNYCTLIGPSFAVEVFENVLTMINVVGPKTRFLKKVSELFNNDTFRLVVNENEHGSELFAALKNVLAIGIGMIDYLRPYRNTMAALLSIGVKEIHMVYKRIFPESNDNMGFELAGIGDIFLTCSSTKSRNFSFGRQVAEFGLEATLKNNKKTIEGYHAAKILANILKKNKDLKIVFLKSIIDILYKNKKPELLLDFVKKYN", "aa_hexdigest": "c28994641dc0ad58ef5021e2f010c03b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XF70", "query_cov": 1.0, "subject_cov": 0.8072289156626506, "identity": 0.996, "score": 514.0, "evalue": 5.07e-183, "valid": true, "product": "Glycerol-3-phosphate dehydrogenase", "uniref50_id": "UniRef50_A0A168RLR2" }, "pscc": { "uniref50_id": "UniRef50_A0A168RLR2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A168RLR2" ], "product": "Glycerol-3-phosphate dehydrogenase" }, "genes": [], "id": "OAIKOHHJFB_1062", "locus": "GCA000209735_01044" }, { "type": "tRNA", "sequence": "contig_1", "start": 583050, "stop": 583124, "strand": "+", "gene": "trnC", "product": "tRNA-Cys(gca)", "amino_acid": "Cys", "anti_codon": "gca", "score": 69.0, "nt": "GACACCATAGCCAAATGGCCAAGGCATGAGTCTGCAAAACTCTGATTACGGGTTCGAATCCCGTTGGTGTCTCCA", "db_xrefs": [ "SO:0000258" ], "anti_codon_pos": [ 583073, 583075 ], "id": "OAIKOHHJFB_10", "locus": "GCA000209735_01045" }, { "type": "tRNA", "sequence": "contig_1", "start": 583129, "stop": 583205, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(acg)", "amino_acid": "Arg", "anti_codon": "acg", "score": 69.4, "nt": "GTACCCATAGCTCAACTGGACAGAGTGTCAGGTTACGGCCCTGAAGGTTGCGGGTTCGACTCCTGCTGGGTACGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 583163, 583165 ], "id": "OAIKOHHJFB_11", "locus": "GCA000209735_01046" }, { "type": "cds", "sequence": "contig_1", "start": 583511, "stop": 584029, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGTTTTTGGATCCTATGATTCAAAAGAATTAGTATTGAATAAAGTTAATAGACTTAATATTGTTCATTTAATTGTTATTTCAGGTTTGCATTTTGACTTATTATTCTATTTAATTTTGTTGATACTAAAACCTTTGGATAAAATTAAAATCAAAAGTAAATATGTGGCTTTTGCCCTCATATTCTATTATTTAACATTACTTGCTAATCCTGTTTCTGCTTTAAGAGCTTATATTATGCAAGTTGTTAAAAATTCAAATAATCCTTTTAGTTATAACAACAAATTTAAAAAATATGATGGTCTAGTAATTTCAATTTTAGTTTTATTTTTACTTAAAAATAATTACTTGTTTTCTTTATCATTTATTCTTAGTTTTTCATCAACTTTTACAATTATGTTATTAGTGCCAATGGTCAAAAGTAAAAAATTAAACAACTTTTTAAAAACATTAATTTTGATGACTTTAATATATCTTTTTAATATTCCAATGCTTTTATATATTAATGAATATTGA", "aa": "MLVFGSYDSKELVLNKVNRLNIVHLIVISGLHFDLLFYLILLILKPLDKIKIKSKYVAFALIFYYLTLLANPVSALRAYIMQVVKNSNNPFSYNNKFKKYDGLVISILVLFLLKNNYLFSLSFILSFSSTFTIMLLVPMVKSKKLNNFLKTLILMTLIYLFNIPMLLYINEY", "aa_hexdigest": "28fa8e154319eeb45cba1d1d4202ac9a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20042.13240000001, "isoelectric_point": 9.810234642028806 }, "id": "OAIKOHHJFB_1063", "locus": "GCA000209735_01047" }, { "type": "cds", "sequence": "contig_1", "start": 584033, "stop": 584416, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTTGACTTTATATTTAAGTTATTTTTTATCTAAAACAAAAATTTATTATGAAAAAGGTATTTATAAAATTGAAGGATCGATAAGCGTCCTAAGCAAAAAATATTTAGTTATATCAAATGAAAATAATAATATTTTAATTTACACAAGTGATTTAAAAAATAGCACTCCTTTAAGTCTAGGGGACCAAGTAGAAATTCAAGGTGAATTAAATCCTATTGAAATAGACAATAGTAAAATAGCAACATTTTATTTGCAAAATAATATAAATTATTTGCTAAAAAAAACAGTCATTTTAACTGTTATTAAACCAGAATTTTCTGTTGAAGAAAAAATAATGAATTATTCAAAAAATCATGGTGAATTATTTGATTCATATTGA", "aa": "MLLTLYLSYFLSKTKIYYEKGIYKIEGSISVLSKKYLVISNENNNILIYTSDLKNSTPLSLGDQVEIQGELNPIEIDNSKIATFYLQNNINYLLKKTVILTVIKPEFSVEEKIMNYSKNHGELFDSY", "aa_hexdigest": "61abfd4da6f33bf809c7d9d8be2e1711", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14675.736699999985, "isoelectric_point": 5.824996376037597 }, "id": "OAIKOHHJFB_1064", "locus": "GCA000209735_01048" }, { "type": "cds", "sequence": "contig_1", "start": 584743, "stop": 585081, "strand": "-", "frame": 2, "gene": null, "product": "MAG0490 family ComEA-like DNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MF63", "UniRef:UniRef90_C4XF72" ], "nt": "ATGAAATATAGAAAGATAATATTAGGAACTTTGTTAGCAGGCACAATTGTAATTTCAACAAGTACAATTGCTTTTAATATTCAAAGAACCAAGGTTGCAAAGGTTAAAAGCAATGATCCTGCATCAATAGGATCAAAATTTTGTATCAAAGTATCCGGTGCAGTTGCCTTTGCTAAAAATTATTATTTCGAAAAGCCTATTTATTTAAAAGAATTATTAGATATGGCTCAACCTTATGAAAATGCAGACTTAAGCAAAATTGACTTAAAAAAAGAATTAAAAGAAAATCTTAATATTTACATTCCATATCAAAAAACTAATAACCCTTTTTTGTATTGA", "aa": "MKYRKIILGTLLAGTIVISTSTIAFNIQRTKVAKVKSNDPASIGSKFCIKVSGAVAFAKNYYFEKPIYLKELLDMAQPYENADLSKIDLKKELKENLNIYIPYQKTNNPFLY", "aa_hexdigest": "b4bb191edb56bd4a657b021e31468864", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 584563, "stop": 585081, "observations": { "mutation": [ 584745 ], "stop": [ 584745 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF72", "query_cov": 1.0, "subject_cov": 0.6511627906976745, "identity": 1.0, "score": 218.0, "evalue": 3.23e-71, "gene_start": 1, "gene_end": 112, "reference_start": 1, "reference_end": 112, "reference_sequence": "MKYRKIILGTLLAGTIVISTSTIAFNIQRTKVAKVKSNDPASIGSKFCIKVSGAVAFAKNYYFEKPIYLKELLDMAQPYENADLSKIDLKKELKENLNIYIPYQKTNNPFLYWNNLSKEHFKLLVDFGIKKSIVNKILDLREKKNQITWNDLENISGVGKVTLKKLQGILIL" }, "paralog": false, "identity": 0.9941860465116279, "score": 335.0, "evalue": 1.78e-119, "description": "Internal stop codon at 584745. Nonsense mutation around 584745.", "psc": { "uniref90_id": "UniRef90_C4XF72", "product": "MAG0490 family ComEA-like DNA-binding protein", "uniref50_id": "UniRef50_A0A3P8MF63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF72", "UniRef:UniRef50_A0A3P8MF63" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MF63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MF63" ], "product": "Competence protein ComEA" } }, "id": "OAIKOHHJFB_1065", "locus": "GCA000209735_01049" }, { "type": "cds", "sequence": "contig_1", "start": 585218, "stop": 585706, "strand": "+", "frame": 2, "gene": "recU", "product": "Holliday junction resolvase RecU", "db_xrefs": [ "EC:3.1.21.10", "KEGG:K03700", "RefSeq:WP_041594224.1", "SO:0001217", "UniParc:UPI00030552D3", "UniRef:UniRef100_C4XF73", "UniRef:UniRef50_C4XF73", "UniRef:UniRef90_C4XF73" ], "nt": "ATGCAAAAAAATAGAGGAATGTTATTAGAAAAAATAATTAATAAAACAATTGATTACTATGCCAAAAATAAATTAGCTTATATTGAAAAGAAGTCTTTACCTGTTAAGTTTAAGAAGGTTAAAAATGATAATTTGAAATTGGATGAGGCTTATATATTAAAGAAGAGCACAGTTGATTACATCGGTTGCTATAAAGGATTTTTTGTTGCTTTTGAAGCAAAAAGCACAAATGAAAAAAGATTGCCAGCAAATAATATTGCTCGGCACCAAATTAAGTATTTATTGGATATTGAAAGTAATAGTGGATTAGCTTTCTTTATTATATTTTTTAGTTTTTGTGATGAGTTTTATCTTGTTAAAGCAAGTGAGTTAATTCAGCATATGAATAAATCTATTTCTTATGAAGAAATAAAAAACATAGGTTTTAGAATCGAATTAACCTTTCCAGGTATAATCGACTTTTTGCCCTATGTTCAAAATTTTTATTAG", "aa": "MQKNRGMLLEKIINKTIDYYAKNKLAYIEKKSLPVKFKKVKNDNLKLDEAYILKKSTVDYIGCYKGFFVAFEAKSTNEKRLPANNIARHQIKYLLDIESNSGLAFFIIFFSFCDEFYLVKASELIQHMNKSISYEEIKNIGFRIELTFPGIIDFLPYVQNFY", "aa_hexdigest": "9ca244e7cf4a86ce48c23f2688042f6a", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI00030552D3", "ncbi_nrp_id": "WP_041594224.1", "uniref100_id": "UniRef100_C4XF73", "db_xrefs": [ "SO:0001217", "UniParc:UPI00030552D3", "RefSeq:WP_041594224.1", "UniRef:UniRef100_C4XF73" ] }, "ips": { "uniref100_id": "UniRef100_C4XF73", "uniref90_id": "UniRef90_C4XF73" }, "psc": { "uniref90_id": "UniRef90_C4XF73", "gene": "recU", "product": "Holliday junction resolvase RecU", "ec_ids": [ "3.1.21.10" ], "uniref50_id": "UniRef50_C4XF73", "kegg_orthology_id": "K03700" }, "pscc": { "uniref50_id": "UniRef50_C4XF73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF73" ], "product": "Holliday junction resolvase RecU" }, "genes": [ "recU" ], "id": "OAIKOHHJFB_1066", "locus": "GCA000209735_01050" }, { "type": "cds", "sequence": "contig_1", "start": 585719, "stop": 585994, "strand": "-", "frame": 1, "gene": null, "product": "DNA-binding protein HU", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FB7", "UniRef:UniRef100_C4XF74", "UniRef:UniRef50_A0A0D5ZK80", "UniRef:UniRef90_C4XF74" ], "nt": "ATGACCAAAAAAGAATTTGTACAAAAAATTGCTGAAATGTTAGAAGTTCCAGTTAGAGTAGCAGATCAATACTTTGATGCATTTCTATTCGTTTTAAAAGAACAACTTATAGCTGAAGATAAAGTTCAACTTTCAGATTTAGGAACCTTCGAAACAAAAATTAGAAGAGGACGTGAAACAATTAATCCATTTACAAATGAACCAATTCAAGTGCCTGAAAAACGTGTTGTAAAATTCACACCTTCAAAATACTTAAGAGATATTGTAAACTTCTAA", "aa": "MTKKEFVQKIAEMLEVPVRVADQYFDAFLFVLKEQLIAEDKVQLSDLGTFETKIRRGRETINPFTNEPIQVPEKRVVKFTPSKYLRDIVNF", "aa_hexdigest": "752c415395f60aa5b520b05f65e60a9d", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001E32FB7", "uniref100_id": "UniRef100_C4XF74", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FB7", "UniRef:UniRef100_C4XF74" ] }, "ips": { "uniref100_id": "UniRef100_C4XF74", "uniref90_id": "UniRef90_C4XF74" }, "psc": { "uniref90_id": "UniRef90_C4XF74", "product": "DNA-binding protein HU", "uniref50_id": "UniRef50_A0A0D5ZK80" }, "pscc": { "uniref50_id": "UniRef50_A0A0D5ZK80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0D5ZK80" ], "product": "Viral histone-like protein" }, "genes": [], "id": "OAIKOHHJFB_1067", "locus": "GCA000209735_01051" }, { "type": "cds", "sequence": "contig_1", "start": 586189, "stop": 586524, "strand": "-", "frame": 2, "gene": "hinT", "product": "histidine triad protein HinT", "db_xrefs": [ "RefSeq:WP_013527111.1", "SO:0001217", "UniParc:UPI0001A8C2AC", "UniRef:UniRef100_C4XF75", "UniRef:UniRef50_A0A4P1QFY6", "UniRef:UniRef90_C4XF75" ], "nt": "ATGCAAAACATATTTCAATTAATAATTAACAAAAAAGCAGAAGCAAATATTATCTTTGAAGATGATAAATGTATAGCTTTTTATGACAAATTCCCAATTCAACCCGGACATTTCTTAGTGGTTCCAAAAAAACACAGTAAAAACATTACTGAAGCAGATGATGAAACAGCTGCACATTTAATAAATGTTGCTCGTAAACTTGGAAAAAAACATGTATTAGATAAGAAAATTGCAGGCTTCAAAATAATAATTAATACAGGCGAAGCTGCTGATCAAACAATTTTCCATACACACGTACACGTAATTCCATATCGTGAAAAAGCACAAGATAACTAA", "aa": "MQNIFQLIINKKAEANIIFEDDKCIAFYDKFPIQPGHFLVVPKKHSKNITEADDETAAHLINVARKLGKKHVLDKKIAGFKIIINTGEAADQTIFHTHVHVIPYREKAQDN", "aa_hexdigest": "ae4ed3bd0dadeae14978ab7253fc607f", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C2AC", "ncbi_nrp_id": "WP_013527111.1", "uniref100_id": "UniRef100_C4XF75", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C2AC", "RefSeq:WP_013527111.1", "UniRef:UniRef100_C4XF75" ] }, "ips": { "uniref100_id": "UniRef100_C4XF75", "uniref90_id": "UniRef90_C4XF75" }, "psc": { "uniref90_id": "UniRef90_C4XF75", "gene": "hinT", "product": "histidine triad protein HinT", "uniref50_id": "UniRef50_A0A4P1QFY6" }, "pscc": { "uniref50_id": "UniRef50_A0A4P1QFY6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4P1QFY6" ], "product": "HIT domain-containing protein" }, "genes": [ "hinT" ], "id": "OAIKOHHJFB_1068", "locus": "GCA000209735_01052" }, { "type": "cds", "sequence": "contig_1", "start": 586985, "stop": 587113, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGGTTCGGAAGATGAAAGTAAAAAAATAAGTGACATTGAAAAAATTACTGATAATGCATACGAAAGTCAATTATTAGGCTATCAAGGTATTAACTTAAACTTCAGTAATTACAACTTGAAATGA", "aa": "MKGSEDESKKISDIEKITDNAYESQLLGYQGINLNFSNYNLK", "aa_hexdigest": "28c62bf59b566fa09c777662ec10cfd6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4799.2388, "isoelectric_point": 4.715101432800293 }, "id": "OAIKOHHJFB_1069", "locus": "GCA000209735_01053" }, { "type": "cds", "sequence": "contig_1", "start": 587705, "stop": 587869, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAAAAAATTATTATTTTCTTTAGGAAGCATTAGCACTTTAGCAGCCTTCCCTATAGCCGCTGTTAGTTGTGGTCGTGTTGTTGAAACTCCAGCTAAAAAAATTCAAGATGATTTCTTAAAAGCTGGCGGCAGAAAAGAAGAAATCAAAAACATTTGA", "aa": "MKNKKLLFSLGSISTLAAFPIAAVSCGRVVETPAKKIQDDFLKAGGRKEEIKNI", "aa_hexdigest": "f1d824eabbbe97e3b76ca09f2e1daa01", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5833.865599999999, "isoelectric_point": 9.806624412536618 }, "id": "OAIKOHHJFB_1070", "locus": "GCA000209735_01054" }, { "type": "cds", "sequence": "contig_1", "start": 588038, "stop": 588361, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGAAATCTTAAAAAATGATTTTATTTTAAGCAAAAAATACAAATCAATTAATGCAACAAAATACAATGCATTAGATGTAATTTCTAAAAACTTAAAATCAGATTATTATGTAAATAAAGTTTTATTAGAAGATGCTGATTTTGTTAAATATCTCAAAGAACAAGAAAATATTTATGCACTTGATGCACAAGGCAAAGCAGTAAAAGGTGTTAAATATTCTGACAAAGATATTGAAGAATTGAAAAAATCGAATGAAATTAAATACAGAATTGATGCTGAACAAAACGCTCTGGATGTTAATAAGAAATTAACAACTTGA", "aa": "MLEILKNDFILSKKYKSINATKYNALDVISKNLKSDYYVNKVLLEDADFVKYLKEQENIYALDAQGKAVKGVKYSDKDIEELKKSNEIKYRIDAEQNALDVNKKLTT", "aa_hexdigest": "6d17360b7967e11d7914c9e665b67152", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12401.071199999984, "isoelectric_point": 8.596230506896976 }, "id": "OAIKOHHJFB_1071", "locus": "GCA000209735_01055" }, { "type": "cds", "sequence": "contig_1", "start": 588470, "stop": 588781, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAGAATTAGTTTATGGATCAACCTCTGTTCCCGAAATTGATTTAAAAGCATTATTTGAAAGTATTCAATCTAAATTAGAAACACTATTCCCTGAATTAGTTCCATTAAAACCAAATTATGATGCTACAGCTTCATTAGAAGAAAAAACTAAAAAAGTTGGAGATTACAACAAAAAACTTCTTGACATCATGAGAGATTTTGATAACAACAAGAAGAGTGGTTTATATGATGACAAATTAAAAGAAGAAATCATACCACTAATTGCTAAAGCTTTTGAAGGTGCTGACAAAAAAATAAGAACATTCTGA", "aa": "MPELVYGSTSVPEIDLKALFESIQSKLETLFPELVPLKPNYDATASLEEKTKKVGDYNKKLLDIMRDFDNNKKSGLYDDKLKEEIIPLIAKAFEGADKKIRTF", "aa_hexdigest": "568dd8127204cced78cae8887d949be6", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11740.414299999984, "isoelectric_point": 5.226254463195799 }, "id": "OAIKOHHJFB_1072", "locus": "GCA000209735_01056" }, { "type": "cds", "sequence": "contig_1", "start": 589088, "stop": 589249, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCATTTACAACAAATAGTTATGTTAAAGCATATAAAGATGCATCTGCATTTATTGATGAATACTATAAAAATGATAATCCATATGCATTCACACAATTTACGATTCCAGGTGTTGCACCTACAAAAATAGCTAAAAAAGGCGAAAAAGAATTAGAATGA", "aa": "MSFTTNSYVKAYKDASAFIDEYYKNDNPYAFTQFTIPGVAPTKIAKKGEKELE", "aa_hexdigest": "10cb30245bb335ec7585f92502734142", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6032.693200000001, "isoelectric_point": 6.175977134704589 }, "id": "OAIKOHHJFB_1073", "locus": "GCA000209735_01057" }, { "type": "cds", "sequence": "contig_1", "start": 589538, "stop": 589672, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCACGTGAAGGCAATGAAGAAGAAAATACTTCATTCAAATTAAAATCAATTGAAGAACTTAAAACAAAAGAAAGAAACAAGATTCTAGATATTCAAGAAAACACCAAAAGAACAGCCGGTTATGATAAATGA", "aa": "MSREGNEEENTSFKLKSIEELKTKERNKILDIQENTKRTAGYDK", "aa_hexdigest": "11e3adff4aa866077e77563db5e5371f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5202.721500000002, "isoelectric_point": 6.228837394714356 }, "id": "OAIKOHHJFB_1074", "locus": "GCA000209735_01058" }, { "type": "cds", "sequence": "contig_1", "start": 589676, "stop": 590077, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAAAGAAAAGAAACATTTTTCGAACGTTTGACAAGAAAAAATGCCGAACACGAATATAAAAATCAACAAGCAATGCCTAAAAAGAAAAAGCTAAAAGGTATAATCCTTGGAACACTTTTAACATCTGCAATTGCAACAGGTATCATTGTTCCAGTTGTAATTAATTCAACAAAAGTAACATATAAAGACGCACTCAAAGATGAAAGTGCTGTTTTAACATTTACATTACCAGGATCAAAAGAAAAACATTCTATTACAATCAAAGAATTAAAAAATTCAAGTAAAACTGAAGATGCTAAAGATCCAAGTAAATTGTTACAACAAATTAAACGTCTTGCGATGTTTTATCTTTATGATAAAGAAGCAAAAGCCTCAGCTGAATATCAAAGATTATGA", "aa": "MAKRKETFFERLTRKNAEHEYKNQQAMPKKKKLKGIILGTLLTSAIATGIIVPVVINSTKVTYKDALKDESAVLTFTLPGSKEKHSITIKELKNSSKTEDAKDPSKLLQQIKRLAMFYLYDKEAKASAEYQRL", "aa_hexdigest": "52ed278054ab1d2b5f2f7733a813549f", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15118.514799999979, "isoelectric_point": 9.835119438171386 }, "id": "OAIKOHHJFB_1075", "locus": "GCA000209735_01059" }, { "type": "cds", "sequence": "contig_1", "start": 590937, "stop": 591104, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAATACAAACGTATATTAGTAAAACTTTCAGGTGAAGGTTTAGTAAATAAACAAAAAAGTTTAGCTATTGACTATGATTTAGTAGAAGACATTGCTAAACAGCTTAAAAAAATTATTGACAAAGGTGTTCAAGTTAGTGTTGTTATTGGTGGAGGTAACTTTTGA", "aa": "MKYKRILVKLSGEGLVNKQKSLAIDYDLVEDIAKQLKKIIDKGVQVSVVIGGGNF", "aa_hexdigest": "467ce7109c83ee61f555fab9336f2359", "start_type": "GTG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6061.142000000002, "isoelectric_point": 9.573829078674315 }, "id": "OAIKOHHJFB_1076", "locus": "GCA000209735_01060" }, { "type": "cds", "sequence": "contig_1", "start": 591162, "stop": 591653, "strand": "+", "frame": 3, "gene": null, "product": "UMP kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P6F8" ], "nt": "ATGTTGGCAACTATCATGAATGGTCTAGCTCTTAAAAGTGGATTCGAAAAAGTTGGATTAAAAACTAGAATCCAATCATCAATTGCAATTGATCAAAAAGTTGCAGAAAATTATATTAATGAAAAGACTTTAAAATACCTTGAAGAAGGTGAAGTAGTTATTTTTGTTGGCGGTACAGGCCGTCCTTACTTTACAACAGATACAGCAGCAACATTATTTGCTTCAGAAATCAAAGCCGAAGTTATCTTAATGGGTAAAAATGGTGTTAGTGGAATCTATGATTCAGACCCTAAGAAAAATCCAAATGCCAAGAAATATGATGTTGTAACTTATGATGAAATTTTAGACAAAAAATTACAAGTCATGGACTTAACGGCCACAAGTATGGCTAGAGACAACAATATTAATTTAATTGTTTTCAACATTAAAGAAAAGAATGCTATTGTCAAAGCTCTTGAAGGTAAAATTGAACACACAGAGGTAATTAAATAA", "aa": "MLATIMNGLALKSGFEKVGLKTRIQSSIAIDQKVAENYINEKTLKYLEEGEVVIFVGGTGRPYFTTDTAATLFASEIKAEVILMGKNGVSGIYDSDPKKNPNAKKYDVVTYDEILDKKLQVMDLTATSMARDNNINLIVFNIKEKNAIVKALEGKIEHTEVIK", "aa_hexdigest": "04f05b835647ab07dc625ab87faab339", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_A0A3B0P6F8", "query_cov": 1.0, "subject_cov": 0.9588235294117647, "identity": 0.595, "score": 204.0, "evalue": 7.06e-65, "valid": false, "product": "UMP kinase", "ec_ids": [ "2.7.4.22" ], "uniref50_id": "UniRef50_A0A3B0P6F8", "kegg_orthology_id": "K09903" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P6F8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P6F8" ], "product": "UMP kinase" }, "genes": [], "id": "OAIKOHHJFB_1077", "locus": "GCA000209735_01061" }, { "type": "cds", "sequence": "contig_1", "start": 591653, "stop": 592204, "strand": "+", "frame": 2, "gene": "frr", "product": "ribosome recycling factor", "db_xrefs": [ "GO:0006412", "GO:0008135", "RefSeq:WP_013354916.1", "SO:0001217", "UniParc:UPI0001A8C2B1", "UniRef:UniRef100_C4XF80", "UniRef:UniRef50_Q98QZ1", "UniRef:UniRef90_C4XF80" ], "nt": "ATGGAATTAGATTTATATTTATTAGATTTTTTAGATGAGGCTGAAAAAGCAATTAATCATTATGCTTTTGAATTATCAAAAATTTCAACTGGACGTGCTAACCCTCAATTAATTAAGGGAGTTAGAATTAATTATTATGATACTTTGACACCTCTTGAAGAACTTGCTTCAATTACAGTACCAGAACCACAACAATTATTAATTAAACCTTTTGACGTTTCATGCAACCATGAAATTGCAAAAACAATTTTAAATGCTAACTTAGGTTTAAATCCTGTTGATGAAGGAAATCAAGTTAGAATAACTTTTCCTCCTCTTACAACTGAAAGACGTAAAGATATGGTTAAGAGTCTTGCAAAATTTACAGAAAATGCTAAAGTTGCAATTAGAAATGCAAGACAAAATGTTAACAAATCTATCAAGGCTGATGAAGAATTATCAGAAGATCAAGAAAAGAAATACTTAGATGAAATTCAAAAAGAAGTTGATAAAAAAATCGAACACATTAACAAAATGACAATCAACAAAGAAAAAGAATTAATGAGCATATAA", "aa": "MELDLYLLDFLDEAEKAINHYAFELSKISTGRANPQLIKGVRINYYDTLTPLEELASITVPEPQQLLIKPFDVSCNHEIAKTILNANLGLNPVDEGNQVRITFPPLTTERRKDMVKSLAKFTENAKVAIRNARQNVNKSIKADEELSEDQEKKYLDEIQKEVDKKIEHINKMTINKEKELMSI", "aa_hexdigest": "256d7dc815b02298fa3ae94b2c0cbff1", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001A8C2B1", "ncbi_nrp_id": "WP_013354916.1", "uniref100_id": "UniRef100_C4XF80", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C2B1", "RefSeq:WP_013354916.1", "UniRef:UniRef100_C4XF80" ] }, "ips": { "uniref100_id": "UniRef100_C4XF80", "uniref90_id": "UniRef90_C4XF80" }, "psc": { "uniref90_id": "UniRef90_C4XF80", "gene": "frr", "product": "ribosome recycling factor", "uniref50_id": "UniRef50_Q98QZ1", "go_ids": [ "GO:0006412", "GO:0008135" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QZ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QZ1" ], "product": "Ribosome-recycling factor" }, "genes": [ "frr" ], "id": "OAIKOHHJFB_1078", "locus": "GCA000209735_01062" }, { "type": "cds", "sequence": "contig_1", "start": 592304, "stop": 592459, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTTTATTAAGAAACTTAAACCATATTTTGAAAAATACAAAAGATCACAAGACAAAATTCATAATTTGATGCTATTAGATTGGTTGATAGCTCTTCTTTTAGTTTTATCATCTGTTATTATGGGAGTTTTACTTCATTATTTAAATAATTGA", "aa": "MLFIKKLKPYFEKYKRSQDKIHNLMLLDWLIALLLVLSSVIMGVLLHYLNN", "aa_hexdigest": "c7b85156445c61eadc330b4514ac14f8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6118.452799999997, "isoelectric_point": 9.698381996154787 }, "id": "OAIKOHHJFB_1079", "locus": "GCA000209735_01063" }, { "type": "cds", "sequence": "contig_1", "start": 592625, "stop": 592777, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAATAATTTAAAAGTTAATCTTAAATTAAAAGAATATAACGAATTAAAAAGATTAATATTTGATTTAGACACAAATATGAAAGAAACTATCCAATCAGTTACTAATAAAGAAAAGATTACATTATTAGATTCAACAGCTTTAATGTGA", "aa": "MLNNLKVNLKLKEYNELKRLIFDLDTNMKETIQSVTNKEKITLLDSTALM", "aa_hexdigest": "cfe4bc8845d516f8858587124dd4f538", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5869.8679, "isoelectric_point": 8.034904289245606 }, "id": "OAIKOHHJFB_1080", "locus": "GCA000209735_01064" }, { "type": "cds", "sequence": "contig_1", "start": 592846, "stop": 593085, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATATAAAATAGAATCACTAAGTGTTTCTTCATTATTTACAAATTGTAATAATTTGAAAATGCTTGATGACATTAGTCAATATCTATTAGGTATATTTACACCACGATATTTTTCAACTAAACTAGTTGGTATTAATTTTGCATATTCAATTTCAACTTGGTTATTGTTAATTTGCCCCACAAAAAGATTTTCTTTGTTTATTATTGCTTTATATTCTTTAATTTTGAAAACATGA", "aa": "MKYKIESLSVSSLFTNCNNLKMLDDISQYLLGIFTPRYFSTKLVGINFAYSISTWLLLICPTKRFSLFIIALYSLILKT", "aa_hexdigest": "8b269059cf18d5face1a4ee872d2143d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9125.817399999989, "isoelectric_point": 9.469454765319824 }, "id": "OAIKOHHJFB_1081", "locus": "GCA000209735_01065" }, { "type": "cds", "sequence": "contig_1", "start": 593177, "stop": 593641, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGCAGCTTTTTATGATCGTAGTGAATTCTTAAATTATTCAATTAGAATGCTTGATATTTATGAAGGAACAAAACTCAAAAACATTTTTACAGCTTTGGAAAACAATGCAAAATTTGGTTTAAAATCACTTAACCTATTTTACGAAAAGGTCATTAAAAAAGTTGTTTTCGACAAAAAAATAGTTGATTTATCACAATCAATTAAAAACACATTATTAGGTCGTGAACTAATACTTAATGAGTATTTTTCATCAACATCACTTAAATCATTTGACAATCATGCAATGTTTTTAAAATTTAAAGACGCAAAAGAAATGAATAAATATTTAAAGAAAATTGAACCAACTAAAATTCATTTTGAAACAAGCAAAGTATTCTTAAATTATCTTTCTCCAAAGGTAAATACAAACAAAGTTGACATTCGTTTTGTTTTAACTAAATTAGTTAACAATTATGTCTAA", "aa": "MFAAFYDRSEFLNYSIRMLDIYEGTKLKNIFTALENNAKFGLKSLNLFYEKVIKKVVFDKKIVDLSQSIKNTLLGRELILNEYFSSTSLKSFDNHAMFLKFKDAKEMNKYLKKIEPTKIHFETSKVFLNYLSPKVNTNKVDIRFVLTKLVNNYV", "aa_hexdigest": "bf7090c90e3190d404d3e8e6769c7931", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18152.047100000003, "isoelectric_point": 9.631077003479003 }, "id": "OAIKOHHJFB_1082", "locus": "GCA000209735_01066" }, { "type": "cds", "sequence": "contig_1", "start": 593673, "stop": 593798, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATTTAGCTAAATTTGTAAAAGACACTAGAAAAGAAATAATAGGAAATAAATCAAAAGCTGATTTTCAAAAATTAATTGATGATTTTAAAAATTTTTTAAAAAGTTTAAAAGTAAAAAAATAA", "aa": "MDLAKFVKDTRKEIIGNKSKADFQKLIDDFKNFLKSLKVKK", "aa_hexdigest": "0157d037a33d4ab7c75ab6e9e8358ce0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4829.7037, "isoelectric_point": 9.919701957702635 }, "id": "OAIKOHHJFB_1083", "locus": "GCA000209735_01067" }, { "type": "cds", "sequence": "contig_1", "start": 593799, "stop": 594053, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF82", "UniRef:UniRef90_C4XF82" ], "nt": "ATGGCAAAAAAGATGAGTAAAGGTGCTATTATTGGTATATCAGTAGGAGCTACAATTTTAGTAGCTAGCACAGTAACCGCTGCTCTTGTGTGTACATTGCATGGAAAAACTGTTTATAAAATTGAATACTCTAAGTATAAAAGAGCTAGAGAAAAAGGCAATATTGGAGACAAAATAGCTTTAGCTACTTTAGAAGGACTTATTGTAACAGGTGAAACAACTTTGAAAGAAATAAATGGAACAAACCAACAATGA", "aa": "MAKKMSKGAIIGISVGATILVASTVTAALVCTLHGKTVYKIEYSKYKRAREKGNIGDKIALATLEGLIVTGETTLKEINGTNQQ", "aa_hexdigest": "ab700fd94d47019bd72d806819e04e14", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 593673, "stop": 594113, "observations": { "mutation": [ 593801 ], "stop": [ 593801 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF82", "query_cov": 1.0, "subject_cov": 0.5753424657534246, "identity": 1.0, "score": 157.0, "evalue": 7.23e-48, "gene_start": 1, "gene_end": 84, "reference_start": 21, "reference_end": 104, "reference_sequence": "MFLYLVVKIVKLLYTFRRCIMAKKMSKGAIIGISVGATILVASTVTAALVCTLHGKTVYKIEYSKYKRAREKGNIGDKIALATLEGLIVTGETTLKEINGTNQQWLDLAKFVKDTRKEIIGNKSKADFQKLIDDFKNFLKSLKVKK" }, "paralog": false, "identity": 0.9863013698630136, "score": 277.0, "evalue": 1.67e-97, "description": "Internal stop codon at 593801. Nonsense mutation around 593801.", "psc": { "uniref90_id": "UniRef90_C4XF82", "uniref50_id": "UniRef50_C4XF82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF82", "UniRef:UniRef50_C4XF82" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF82", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF82" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8881.345699999996, "isoelectric_point": 9.673819541931152 }, "id": "OAIKOHHJFB_1084", "locus": "GCA000209735_01068" }, { "type": "cds", "sequence": "contig_1", "start": 594157, "stop": 594477, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATACGTCAATTTATACAATACAAGTGAATACACTTTTTTAGATTCTTTAATTAGAATAGATGATTTAGTTAGATTATCAAAAGAACACAATCTTCCTGCAGTAGTTTTAAGTGACCGAAACAATATGTTCGGTTTAGGTGCTTTTATGGAAGCATGCTTAAAATATGATATCAAAGGTATCATTGGAGTGGACTTAGATGTAGACAATTATCGCTTCATAGTCTTAGCGAAAAACTACCAAGGTTACCAAAAAATTAATCAACTAATTTATCAAAAAAGTATTAATAAAGCTATTGAAATTAGTGAATTATGA", "aa": "MKKYVNLYNTSEYTFLDSLIRIDDLVRLSKEHNLPAVVLSDRNNMFGLGAFMEACLKYDIKGIIGVDLDVDNYRFIVLAKNYQGYQKINQLIYQKSINKAIEISEL", "aa_hexdigest": "9686477b1aa1f35787b12f70e3cf0973", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12302.101399999989, "isoelectric_point": 6.556229972839356 }, "id": "OAIKOHHJFB_1085", "locus": "GCA000209735_01069" }, { "type": "cds", "sequence": "contig_1", "start": 595264, "stop": 597045, "strand": "+", "frame": 1, "gene": null, "product": "DNA-directed DNA polymerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D3VPK4", "UniRef:UniRef50_UPI00244ECC9B", "UniRef:UniRef90_C4XF83" ], "nt": "ATGGCAATTAGAGATATGGGCAGAGTTATGGGAATTAGCTTAATTGACATCAACCGCATCAGTAAAACTATTAAATCAGATAACTTAACTGATGCTTATAAAAATGATATTGACTTTAAAATTGAAATGGATAATTATCCTGAGTTATATAAACATGCACTTAAAATCGAAGGCTTACCTCGTCAACAAAGTTTCCATGCAGCAGGTTTTATTATTGCTAAGGACAAATTATTTAATTATGTCCCTGTTTGTTTAAGCAATTTAGACAATTACCAACAGGTACAAGTACCAATGAATTACATAGAGAGTTATGGACTTTTAAAAATTGACTTGCTAGGTTTAAGAACTTTAACTGAAATTCAGCTAATTGAAAAAAGTTTAGAAGAAAACCAAAAATTTGATTATTTAGTAAAAAACGATCCTCAAATTTTAAATGATCCTAAAACTTTAACAATGCTCAACTTAGGTTTTACTGAAGGAATATTTCAATTAGAATCTCCGGGCATGAAGAAGACAATTCAAAAAGTTGTCTTGAATTCTTTTGATGATTTATATGCCATTATTTCATTATTTAGACCTGGACCTATGGACTTTATCAATGACTATGCAACTAATAAGAAAAATCAAAAATTAATTAAAAAAATACATCCTGATTATGACAAAGTTGTTGCCAACACATATGGCATAATAGTTTATCAAGAACAAATTATGCAAATAGCTCAAAGTATTGGCGGAATGACTTTTGCTCAAGCTGATTTATTGCGTAAAGCAATTAGCAAAAAAAGTGAAGTTGATATCAAAAAATATAGAAGCATATTTTTTGAAAATGCCATTAAAAAAGGCATAAACGAAAAAATTATTAATGCTATTTATAACAATATTGAAAAATTTGGACTATACGGTTTTAATAAATCTCATGCTGTTAGTTATGCATATTTAACTATGAAAATGTCATACTATAAAGCTCGTTATGCTAACTTATTTTATAGCTCTTTAATTACAAATGCAGCTGGAGCACAAGCTACAATTAATAAATATGTAGCTGAAATTAAGAAACTTAATTTTCCAGTTTATTCGCCAAATATTTTGCATTCAAGTAATAAATGTATAGTAACAAACGATGGCATTTATTTACCTTTTGAAATGATTAAAGGTTTTGGAGCTGAAGGTGTTAATAAAATTGTGGCTGATTTAAATGAAAATGGACCATTTCAAAAGAATTTAATTGATACAATGCTTAGACTTCGTTTTGCAGGTATTAAAGATGCTGCTATGGAAATATTAATTAAAGCCAATGCTTTTAGAGACTTTGGACACATGAAATATATACTATCTTGTGATAATCAAATTAAAGACATTTTTAAGGTTTTATCTTCACATTCAACTTATAAAGAAGCATTAAAAGATATTCAAAAAATGGGTTACTATGATATAGTTTTTGATCAAGAAAATGAACGTGACATAAATTACGAAATGGACTATGAAATAGCTCTTTTAGGTGAATTATACAATGCTTTTAGTACTTCAATTTATGAAGCAAAATACAAAAATAAATTAAATACAATAACCTCTTCAATTCCTGTTTCTGTTGCTGCTGAAGTAGCTGATTTAAGAAGACCAAGAGGTAAAGAATTTAGCGTTTTAGAATTAAGAGATAGTACAGCTAAAATTACTTTCTTTGTTAACAACAAAGTATTGGCAAGCTCACTTCCTCTTAAAAAAGGACAAATAATTGAAGCTACAATTAGTTTCAGTCGCAACAAACATAATTTAATTAGCTGA", "aa": "MAIRDMGRVMGISLIDINRISKTIKSDNLTDAYKNDIDFKIEMDNYPELYKHALKIEGLPRQQSFHAAGFIIAKDKLFNYVPVCLSNLDNYQQVQVPMNYIESYGLLKIDLLGLRTLTEIQLIEKSLEENQKFDYLVKNDPQILNDPKTLTMLNLGFTEGIFQLESPGMKKTIQKVVLNSFDDLYAIISLFRPGPMDFINDYATNKKNQKLIKKIHPDYDKVVANTYGIIVYQEQIMQIAQSIGGMTFAQADLLRKAISKKSEVDIKKYRSIFFENAIKKGINEKIINAIYNNIEKFGLYGFNKSHAVSYAYLTMKMSYYKARYANLFYSSLITNAAGAQATINKYVAEIKKLNFPVYSPNILHSSNKCIVTNDGIYLPFEMIKGFGAEGVNKIVADLNENGPFQKNLIDTMLRLRFAGIKDAAMEILIKANAFRDFGHMKYILSCDNQIKDIFKVLSSHSTYKEALKDIQKMGYYDIVFDQENERDINYEMDYEIALLGELYNAFSTSIYEAKYKNKLNTITSSIPVSVAAEVADLRRPRGKEFSVLELRDSTAKITFFVNNKVLASSLPLKKGQIIEATISFSRNKHNLIS", "aa_hexdigest": "214f59e33a5be4b722ab07b28a5be231", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00244ECC9B", "query_cov": 0.8768971332209107, "subject_cov": 0.9683426443202979, "identity": 0.5, "score": 482.0, "evalue": 4.67e-162, "valid": false, "uniref50_id": "UniRef50_UPI00244ECC9B" }, "pscc": { "uniref50_id": "UniRef50_UPI00244ECC9B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00244ECC9B" ] }, "genes": [], "truncated": "both", "pseudogene": { "start": 594964, "stop": 597060, "observations": { "mutation": [ 594994, 597043 ], "stop": [ 594994, 597043 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF83", "query_cov": 1.0, "subject_cov": 0.6132368148914168, "identity": 1.0, "score": 1156.0, "evalue": 0.0, "gene_start": 1, "gene_end": 593, "reference_start": 370, "reference_end": 962, "reference_sequence": "MKKYVNLYNTSEYTFLDSLIRIDDLVRLSKEHNLPAVVLSDRNNMFGLGAFMEACLKYDIKGIIGVDLDVDNYRFIVLAKNYQGYQKINQLIYQKSINKAIEISELWNPNLIVLDHPRYGYYAQTKDNKYSASKNYYINSSDASLPNSILIKENKLFEASDNETLNYLQKLGNLEETKHYANYFDEVNVDSEIIDRIYSIINECNVIFPKKALHLAPFQNNDEATNEKLFVSLLKAGAKRLASELKNYPDWNKRLEYEFNVIKKLGYINYFLIIQDLVNWAKSQKIAIGPGRGSAAGSLVSYLLNITEINPLRYNLLFERFLNPDRVSWPDIDIDIQDDRRFEVFEYLQKKYGVDKTGLISTFQTIGAKMAIRDMGRVMGISLIDINRISKTIKSDNLTDAYKNDIDFKIEMDNYPELYKHALKIEGLPRQQSFHAAGFIIAKDKLFNYVPVCLSNLDNYQQVQVPMNYIESYGLLKIDLLGLRTLTEIQLIEKSLEENQKFDYLVKNDPQILNDPKTLTMLNLGFTEGIFQLESPGMKKTIQKVVLNSFDDLYAIISLFRPGPMDFINDYATNKKNQKLIKKIHPDYDKVVANTYGIIVYQEQIMQIAQSIGGMTFAQADLLRKAISKKSEVDIKKYRSIFFENAIKKGINEKIINAIYNNIEKFGLYGFNKSHAVSYAYLTMKMSYYKARYANLFYSSLITNAAGAQATINKYVAEIKKLNFPVYSPNILHSSNKCIVTNDGIYLPFEMIKGFGAEGVNKIVADLNENGPFQKNLIDTMLRLRFAGIKDAAMEILIKANAFRDFGHMKYILSCDNQIKDIFKVLSSHSTYKEALKDIQKMGYYDIVFDQENERDINYEMDYEIALLGELYNAFSTSIYEAKYKNKLNTITSSIPVSVAAEVADLRRPRGKEFSVLELRDSTAKITFFVNNKVLASSLPLKKGQIIEATISFSRNKHNLISWKGIQ" }, "paralog": false, "identity": 0.997134670487106, "score": 1367.0, "evalue": 0.0, "description": "Internal stop codon at 594994, 597043. Nonsense mutation around 594994, 597043.", "psc": { "uniref90_id": "UniRef90_C4XF83", "product": "DNA-directed DNA polymerase", "uniref50_id": "UniRef50_D3VPK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF83", "UniRef:UniRef50_D3VPK4" ] }, "pscc": { "uniref50_id": "UniRef50_D3VPK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D3VPK4" ], "product": "DNA-directed DNA polymerase" } }, "id": "OAIKOHHJFB_1086", "locus": "GCA000209735_01070" }, { "type": "cds", "sequence": "contig_1", "start": 597060, "stop": 597389, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02739.22" ], "nt": "ATGAACAAAGACAACTTTTTATTAATAGATGGGAATTATTTATTATTTTCTTCTTTCTATGCTTCATATAATCCGGATAGATTAGAAAATATTATGCGTTCACCACAAGGTTTAACAACTAATGGTGTTCATGTTTTTCTTATGACTTTAAGTAAACTAATAGCATACTTTAAACCTAAATATTTATTTATTGCTTTTGATGCTTATGGAGAAACTAGAAGACATAAAATATTCAAAGATTATAAAGCAGGTAGAACCAAAGCACCAGAAATAATTTTTGAACAATTTAGCTTAATTAAAGAAATTTTGACAAAATTAAATATCAAATGA", "aa": "MNKDNFLLIDGNYLLFSSFYASYNPDRLENIMRSPQGLTTNGVHVFLMTLSKLIAYFKPKYLFIAFDAYGETRRHKIFKDYKAGRTKAPEIIFEQFSLIKEILTKLNIK", "aa_hexdigest": "12525ab94bd27556be50f62534d62207", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02739.22", "name": "5_3_exonuc_N", "length": 104, "aa_cov": 0.9541284403669725, "hmm_cov": 0.9326923076923077, "evalue": 5.76799139748839e-27, "score": 90.50469970703125, "start": 6, "stop": 109 } ], "seq_stats": { "molecular_weight": 12796.798399999981, "isoelectric_point": 9.583112525939939 }, "id": "OAIKOHHJFB_1087", "locus": "GCA000209735_01071" }, { "type": "cds", "sequence": "contig_1", "start": 597720, "stop": 597944, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATCAAAATATTGATCAAATTAAAGGCAAAACTAAAGAAAAATTATTAGATGAAAAAGATCAAGCTTTTTTATGTAAAGCATTAGCAAAATTAAATTTAAATGTTGATATGAATAAAAACATTTCTGATTACCTATTGAACTTAAACTTAGAAGATGGCTTAGATGCAATGCGTTTTTATGGTTTAAATAAATGCGCAAATTTATTTGAAGAATTGAAATAA", "aa": "MYQNIDQIKGKTKEKLLDEKDQAFLCKALAKLNLNVDMNKNISDYLLNLNLEDGLDAMRFYGLNKCANLFEELK", "aa_hexdigest": "37366898112e2908f1ef2962eab44d26", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8586.866399999995, "isoelectric_point": 5.294802284240721 }, "id": "OAIKOHHJFB_1088", "locus": "GCA000209735_01072" }, { "type": "cds", "sequence": "contig_1", "start": 597993, "stop": 598118, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATATAATAAATTAAGTTTTATTTTTGAATGCAATAATGGAACATACACATTAATTCCCGACAAAGTACCTTACTTTTACGATGGTAATTATTTAACATATAATATAACAAAAATAAAATAG", "aa": "MEYNKLSFIFECNNGTYTLIPDKVPYFYDGNYLTYNITKIK", "aa_hexdigest": "cfe6e13862270492afbf66182e259e5d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4935.5799000000015, "isoelectric_point": 5.967207527160644 }, "id": "OAIKOHHJFB_1089", "locus": "GCA000209735_01073" }, { "type": "cds", "sequence": "contig_1", "start": 598173, "stop": 598397, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATCAAAAACATTAAATCCTATTTACAATATTACAAAAAATATGGACTTGTTTAGAGCTGAAAATTATATGGACACTTTAGATATTAATACATTTAATTTTGAATTAACAGAGAAAAGTTTGATGGGATTTAAAACAATCAGATGTTTAAAAGATGATTTTGAAATTTTCAAAGAAAAAAATATGGAAAAAACAACAAATAATAATGGAGAAGAAGTTTGA", "aa": "MESKTLNPIYNITKNMDLFRAENYMDTLDINTFNFELTEKSLMGFKTIRCLKDDFEIFKEKNMEKTTNNNGEEV", "aa_hexdigest": "8f8106aa575368676c84cbbdeeb297ed", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8811.912299999993, "isoelectric_point": 4.732835197448731 }, "id": "OAIKOHHJFB_1090", "locus": "GCA000209735_01074" }, { "type": "cds", "sequence": "contig_1", "start": 598698, "stop": 599522, "strand": "-", "frame": 3, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF85", "UniRef:UniRef90_C4XF85" ], "nt": "TTGAAAAAAGTCAAAAAAGAAATAGAAAATGAAATTAAAATAAGAGAAAATAAAAGTGATGTTATTTCTTTTAAAAATTTTTGTATTTCTTCAAATTTCTTTTCTTTAGAAACTAATGATTTGCAAAATCCTAAATTTATGAAAAGCATGTTAAGACTTTCTTTGTTGAAAGACTGCAATGTCAACTTTGACTACAGCACTCGAGATACATTCTATTTCTATAAAAGAATTAATATAAAATTATCAGAATTTAATAATGTTTTAATTAGCTCATTCAACAATTCAAAATATTCTAAAAATTACATGTCAGGGATTTATTTCTATTTAAATAATTTTGTAGCAGAGAAACAAAATATTATTGTTGAAAAAAATAATTTTAATTATCGTGAAGCAAATCAATTTCAAAATGTAAAAATAAATAATTTAACATTAAATAATTTTAAACCAGACTTTGAAAACGAATACATTATAGCCAAAAAACTTGAAGTTAATACTATAGAATTTGGTGATGACTTTCTGAATAATGTTCATATAGAAGAAACGTTTAAAAATATAGATTTTAATGACATAAAAGGCGTTCAAAATGTATTAAAAAATTTAGATTGTTCGAAATCGAAAAATAAAGTTTTTCTATTCCAAAAATTAAATAAAATAAATAGAATGCCAGATTCATTTAAAATTAATTTAAGAGAAGAAACATTATCTGATTTTAGTACTTTATGGATGGGCAATTTAGATACCATTTTAGGCGCATGTAAAGATACTCTTGACTTATCAGAATCAAGTTTAATAAAATGTTTATCTTTTGATGATTATGGTATCTGA", "aa": "MKKVKKEIENEIKIRENKSDVISFKNFCISSNFFSLETNDLQNPKFMKSMLRLSLLKDCNVNFDYSTRDTFYFYKRINIKLSEFNNVLISSFNNSKYSKNYMSGIYFYLNNFVAEKQNIIVEKNNFNYREANQFQNVKINNLTLNNFKPDFENEYIIAKKLEVNTIEFGDDFLNNVHIEETFKNIDFNDIKGVQNVLKNLDCSKSKNKVFLFQKLNKINRMPDSFKINLREETLSDFSTLWMGNLDTILGACKDTLDLSESSLIKCLSFDDYGI", "aa_hexdigest": "b2bddd44fc927f4b317fcd6dcfbd543a", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 598395, "stop": 599819, "observations": { "mutation": [ 598439, 598544, 598598, 598700, 599597, 599747 ], "stop": [ 598439, 598544, 598598, 598700, 599597, 599747 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF85", "query_cov": 1.0, "subject_cov": 0.4120300751879699, "identity": 0.996, "score": 534.0, "evalue": 1.1e-185, "gene_start": 1, "gene_end": 274, "reference_start": 157, "reference_end": 430, "reference_sequence": "MKKNKIFNSLLLTPLIITPITSCGSKPEEKKSFPLPRSCTDYDKKELNKIVEKNTKWIENSKTHKLVKYIDYDFFYDVVKKWYCDDNNNLKSDNFYFPVLDKLSVLQKEDDIALVSSYIFSTKKDLQEELDWINNNEKKAIQKYSKRNETLSDEENLKKVKKEIENEIKIRENKSDVISFKNFCISSNFFSLETNDLQNPKFMKSMLRLSLLKDCNVNFDYSTRDTFYFYKRINIKLSEFNNVLISSFNNSKYSKNYMSGIYFYLNNFVAEKQNIIVEKNNFNYREANQFQNVKINNLTLNNFKPDFENEYIIAKKLEVNTIEFGDDFLNNVHIEETFKNIDFNDIKGVQNVLKNLDCSKSKNKVFLFQKLNKINRMPDSFKINLREETLSDFSTLWMGNLDTILGACKDTLDLSESSLIKCLSFDDYGIWGQSFFKTKKIIFPKKIKEIEGQIYPGSFVNIKFWENIGFTSDNFKLFFGRNWRLDEDKQIEIYSFDSSKKFEVLNDAGIFLKDNGEWENKKIRLTIKIDYMESKTLNPIYNITKNMDLFRAENYMDTLDINTFNFELTEKSLMGFKTIRCLKDDFEIFKEKNMEKTTNNNGEEVWYFKNHADYFNVPVNTYPNMEYNKLSFIFECNNGTYTLIPDKVPYFYDGNYLTYNITKIK" }, "paralog": false, "identity": 0.9873417721518988, "score": 931.0, "evalue": 0.0, "description": "Internal stop codon at 598439, 598544, 598598, 598700, 599597, 599747. Nonsense mutation around 598439, 598544, 598598, 598700, 599597, 599747.", "psc": { "uniref90_id": "UniRef90_C4XF85", "product": "Lipoprotein", "uniref50_id": "UniRef50_C4XF85", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF85", "UniRef:UniRef50_C4XF85" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF85", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF85" ], "product": "Lipoprotein" } }, "id": "OAIKOHHJFB_1091", "locus": "GCA000209735_01075" }, { "type": "cds", "sequence": "contig_1", "start": 599820, "stop": 599990, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAATAAAATTTTTAATAGTTTATTATTAACTCCATTAATTATAACTCCTATAACATCTTGTGGTAGTAAGCCAGAAGAAAAAAAGTCATTCCCATTACCCAGATCTTGCACAGATTATGACAAAAAAGAATTGAATAAAATAGTTGAAAAAAATACAAAATGA", "aa": "MKKNKIFNSLLLTPLIITPITSCGSKPEEKKSFPLPRSCTDYDKKELNKIVEKNTK", "aa_hexdigest": "00075997bb042fceeb69ba3094980dd5", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6409.538000000002, "isoelectric_point": 9.597360038757323 }, "id": "OAIKOHHJFB_1092", "locus": "GCA000209735_01076" }, { "type": "tRNA", "sequence": "contig_1", "start": 600151, "stop": 600237, "strand": "+", "gene": "trnL", "product": "tRNA-Leu(taa)", "amino_acid": "Leu", "anti_codon": "taa", "score": 67.7, "nt": "GCCCAGGTGGCGGAATAGGTAGACGCAAGGGACTTAAAATCCCTCGAACGTTAGTTCGTGCTGGTTCAAGTCCAGTTCTGGGTACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 600185, 600187 ], "id": "OAIKOHHJFB_12", "locus": "GCA000209735_01077" }, { "type": "tRNA", "sequence": "contig_1", "start": 600266, "stop": 600341, "strand": "+", "gene": "trnK", "product": "tRNA-Lys(ttt)", "amino_acid": "Lys", "anti_codon": "ttt", "score": 78.2, "nt": "GCGTCCTTAGCTCAGGCGGTAGAGCAAATGGCTTTTAACCATTGGGTCGGAGGTTCGAGTCCTCTAGGACGTACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 600298, 600300 ], "id": "OAIKOHHJFB_13", "locus": "GCA000209735_01078" }, { "type": "cds", "sequence": "contig_1", "start": 600540, "stop": 600890, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGGGCATTAGTCCTCCCTAAAATATTTGGCGACTTGTCGCCCTTTCTAAAATATTATTTTAATTTTTTTATTTTTTTGAAAAACAAATTTCTATTCTTGTTACTTTCTCAGTTTCTCTTTTGTTTTCCATATAATACTTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCGTACATTTCCAAAGCAGATAAATATCCAAAAATTTCTCTTGGACGATTGTTTATTTCATTTTGTACTTTTAGAATTTCTTCTTCAGTAATATCATCAAAATTAGTTTTTTTCTTAAAAAATCTTCTAACTAAACCATTGAAATTTTCATTTGAACCCCTTTGA", "aa": "MRALVLPKIFGDLSPFLKYYFNFFIFLKNKFLFLLLSQFLFCFPYNTLSIPVEFQGFSAFNFSYISKADKYPKISLGRLFISFCTFRISSSVISSKLVFFLKNLLTKPLKFSFEPL", "aa_hexdigest": "097b322b767dca169ee8e2a053647a07", "start_type": "GTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13641.180399999976, "isoelectric_point": 9.912288093566893 }, "id": "OAIKOHHJFB_1093", "locus": "GCA000209735_01079" }, { "type": "cds", "sequence": "contig_1", "start": 602084, "stop": 602206, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAATAGGCGACAACTTTAATGACTTCTTTGATAGAAACTCAAAAGGTAAAGACAATGACAAAAGAACTCAACAATACGTTAAAAACACAGACATGAGAAAATTATTCACAGATTATTAA", "aa": "MRIGDNFNDFFDRNSKGKDNDKRTQQYVKNTDMRKLFTDY", "aa_hexdigest": "b8fbc57b438c5c1af0b50d8b6eb02fc8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4923.374200000001, "isoelectric_point": 9.221509361267088 }, "id": "OAIKOHHJFB_1094", "locus": "GCA000209735_01080" }, { "type": "cds", "sequence": "contig_1", "start": 602357, "stop": 602497, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTGGTTCAATGCCTTATGTAGCAATGGATGGAACTAAAGTTATTACAGACGAAATGACAAAAGATATGTCTAAGGGTGATCTTAAAAGCCTAGCTGAAAGAATGAAATTTACAAAAACTTTTGAAACTACACCATGA", "aa": "MRGSMPYVAMDGTKVITDEMTKDMSKGDLKSLAERMKFTKTFETTP", "aa_hexdigest": "5e824ebab18077695bfcb7de0d322eaa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5206.043, "isoelectric_point": 8.064495277404784 }, "id": "OAIKOHHJFB_1095", "locus": "GCA000209735_01081" }, { "type": "cds", "sequence": "contig_1", "start": 602498, "stop": 602872, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATATGCTGTAACCGATGGTTTTGAATATGGTCAAAACTTTAATACAACTAAACAAGTAAATAATGCTGCAACAGATAAATACATTCCAGTTATCTTTATGGACATTGATGAAACTGTTTTACAAAATGACTACACAGAAACATTTGGTATGTTAAATGGTGGTTACAATGGAGACACGAAAGAAAAGAACGACCTTAAAGGAAATAGATTTGCTGTGCCAGGCGCTATTGATTTCATTAACTATGTACAATCAAAAGGTGCTTTAGTTGTTTATAACTCAGATATGAACCAATCTACAGCAGTTAGAGAAGCTGTTAAAAAGAATTTGATAAACTTAGGTGTTAAATATGTTTCAGACTTCCAATTCTGA", "aa": "MKYAVTDGFEYGQNFNTTKQVNNAATDKYIPVIFMDIDETVLQNDYTETFGMLNGGYNGDTKEKNDLKGNRFAVPGAIDFINYVQSKGALVVYNSDMNQSTAVREAVKKNLINLGVKYVSDFQF", "aa_hexdigest": "42ac7cfd0b6ef03e36ecec4ef2d7b217", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13921.399399999984, "isoelectric_point": 4.818377876281737 }, "id": "OAIKOHHJFB_1096", "locus": "GCA000209735_01082" }, { "type": "cds", "sequence": "contig_1", "start": 602948, "stop": 603187, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAAGATTTTTATTAGGTGCTTTAGCTCCAGTTGTTGCTTTAAGCCCTATTGCTATTGCAGTTAGTTGTGGAAAACAACCAACCATTGAAGAACAATTCGCTAAATTAAATAATGAAGGAAAATTAGCCTTTATTAATGCCATTGACTACAAAGGAATTATGTCAATGGATGAAAAAGAAAAATTAATTTCTAAATTAAATAAAGATGCTGCTCAATTTGGATCAATTGTTTGA", "aa": "MKKRFLLGALAPVVALSPIAIAVSCGKQPTIEEQFAKLNNEGKLAFINAIDYKGIMSMDEKEKLISKLNKDAAQFGSIV", "aa_hexdigest": "55e34f3d3ff1294323d022cb6eb7df7a", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8567.070599999995, "isoelectric_point": 9.167484855651857 }, "id": "OAIKOHHJFB_1097", "locus": "GCA000209735_01083" }, { "type": "cds", "sequence": "contig_1", "start": 603360, "stop": 603575, "strand": "-", "frame": 3, "gene": null, "product": "MAG6790 family protein", "db_xrefs": [ "RefSeq:WP_013354908.1", "SO:0001217", "UniParc:UPI0001E32FAB", "UniRef:UniRef100_C4XF88", "UniRef:UniRef50_C4XF88", "UniRef:UniRef90_C4XF88" ], "nt": "ATGTATAAATTTAAAGCAAAATTAGTTTCAACTCAAGAAGTTGTTGCACAAGCAAACTCACTTGAAGAAATCGAAGGATTAATCCTTGGCTTCCGTCGTAAACAAAAATATGACGAACACACAAGAGCCAACGAAAAAATCCAAATTATACATGTTGAAAGGGATTCTTTAAAAGGTAAACATAAATCAAAAGAAGAAATTCTTAAAGTTGTTTAA", "aa": "MYKFKAKLVSTQEVVAQANSLEEIEGLILGFRRKQKYDEHTRANEKIQIIHVERDSLKGKHKSKEEILKVV", "aa_hexdigest": "3aa7ea5bd06dae26f05da1144afc132b", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32FAB", "ncbi_nrp_id": "WP_013354908.1", "uniref100_id": "UniRef100_C4XF88", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32FAB", "RefSeq:WP_013354908.1", "UniRef:UniRef100_C4XF88" ] }, "ips": { "uniref100_id": "UniRef100_C4XF88", "uniref90_id": "UniRef90_C4XF88" }, "psc": { "uniref90_id": "UniRef90_C4XF88", "product": "MAG6790 family protein", "uniref50_id": "UniRef50_C4XF88" }, "pscc": { "uniref50_id": "UniRef50_C4XF88", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF88" ], "product": "Transposase" }, "genes": [], "id": "OAIKOHHJFB_1098", "locus": "GCA000209735_01084" }, { "type": "cds", "sequence": "contig_1", "start": 603890, "stop": 604201, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTGTTTTGTTTCATTGTTTGTTGCTGTTTATTTACTTTTAAATAATAAAAATGAAACAATAAATAAAAATAATAATTTAGTTAAAATAAAAATACAAAGTAAAGAAGATAAAATAGGACTTATAAATCAAGATAAATATATTGAACAATATGCAAAAAGTCCTTCTTCATTAAATAATTTTGACAATGTTACAAATGATTTAAAAAATCAATTTATTGATTTAAAATTTAATGATTATATTTTGGCTAATAACACTGTTTTAAAGTATGATACGAAAAAACATAGCTATAAATATGATAAGAGAAATTGA", "aa": "MCFVSLFVAVYLLLNNKNETINKNNNLVKIKIQSKEDKIGLINQDKYIEQYAKSPSSLNNFDNVTNDLKNQFIDLKFNDYILANNTVLKYDTKKHSYKYDKRN", "aa_hexdigest": "b49c3b105b6b078a6e81cf5fca7a2304", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12145.707299999984, "isoelectric_point": 9.256064414978024 }, "id": "OAIKOHHJFB_1099", "locus": "GCA000209735_01085" }, { "type": "cds", "sequence": "contig_1", "start": 604370, "stop": 604576, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTTATGGACCGGAAATAAAATTTTTAGATTCTATAAACGTTAATGACTTTAGTATTATCACTAAAGAATCTGCAGGTATTTATTTGCCAACAATTCAAAGAATTTATTTAAATGGTGCGGCACTAAGTGAAAAAGGAGCAAAATTAAGTGTCAAAGTAAAACAAATACTTTATTCACTTTTTCATGAATACATGCACCATTGA", "aa": "MTYGPEIKFLDSINVNDFSIITKESAGIYLPTIQRIYLNGAALSEKGAKLSVKVKQILYSLFHEYMHH", "aa_hexdigest": "356b010b2c3ef79291ab8d8f2b341330", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7746.9088, "isoelectric_point": 8.02297763824463 }, "id": "OAIKOHHJFB_1100", "locus": "GCA000209735_01086" }, { "type": "cds", "sequence": "contig_1", "start": 604865, "stop": 605005, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATCTATTAAAGATGAAAATGACAAAGTAACTTTTAGAGAAACTAAAGATTTCGATTCAAAATATTTAATAGCCGATTATTCAATAAATAGTTTGCCTTATTATTATTCTCTAGTTGAACTAGTGCCAAGAGAATGA", "aa": "MKSIKDENDKVTFRETKDFDSKYLIADYSINSLPYYYSLVELVPRE", "aa_hexdigest": "574ec02ac4110de8a8b850d17618d522", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5525.1548, "isoelectric_point": 4.920460700988768 }, "id": "OAIKOHHJFB_1101", "locus": "GCA000209735_01087" }, { "type": "cds", "sequence": "contig_1", "start": 605192, "stop": 605659, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATAAAGCTCAATTAGAAGCTGATCGAAACTTTTATAGTAACAATATTTATAAAAGTGAAAATCAACAACTATTTTACAAATTGTTTCTTGAAACAATGGGTTATGGTAAAACTATAGCTCAAGTTAAAAGCAAAATAGATGTTAAAAAATTAATTGCTGGAGACACTGATAGTTATTCAGTTGAAATTGATAAAAATAATATTTTTCAAACAAGAATTAGTGGTTATTTAGATAACAAAAATATCAAAGGATTTTATTTCACAAATAATAAAGATGAAAAAGAGTTTGTTAAAATAAATTATTTAAATGCATTTAAATTTGAGGGAAAAGATAATTTATTAGATACTACTTTTACCTTATCGCCAAATATGGTTTTAAGTGATTTAATTTCAAAACAAGCAGCTTATACAAGTGATTATTTTGATTATAGAAATATAAAAGAAAATTCACCTATTGAATTCTGA", "aa": "MHKAQLEADRNFYSNNIYKSENQQLFYKLFLETMGYGKTIAQVKSKIDVKKLIAGDTDSYSVEIDKNNIFQTRISGYLDNKNIKGFYFTNNKDEKEFVKINYLNAFKFEGKDNLLDTTFTLSPNMVLSDLISKQAAYTSDYFDYRNIKENSPIEF", "aa_hexdigest": "02e5941cf22b06206b414c561ecac536", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18136.189799999986, "isoelectric_point": 6.595448875427247 }, "id": "OAIKOHHJFB_1102", "locus": "GCA000209735_01088" }, { "type": "cds", "sequence": "contig_1", "start": 605684, "stop": 605878, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGATAATGAATTAATCAAAGAATATGAAAAAGATCCATTGCCAGATAGATATTTAACTAGTAGTGATGCACCTAGTGAATTAGATTCAACGAATTCATATAAAATAGAAGCTAGCTTTATAAGCAAAAAACAATCAAATAAAAAATATGGAATATATTTTAAAAGGAGGGAAATGAATGTCTTTAGTTAA", "aa": "MQDNELIKEYEKDPLPDRYLTSSDAPSELDSTNSYKIEASFISKKQSNKKYGIYFKRREMNVFS", "aa_hexdigest": "70ef24e96a18522dcb8d8efb93d804de", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7575.3679999999995, "isoelectric_point": 6.205590248107909 }, "id": "OAIKOHHJFB_1103", "locus": "GCA000209735_01089" }, { "type": "cds", "sequence": "contig_1", "start": 605865, "stop": 605999, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTTTAGTTAATAAGGATAATCTTCTTATTAAAGAAGAAAATTTAAAACCTAAAAAAATAGTTTTTCCCTTAATTGGATCAATTATTAATGTCCAAGCTTTTAAATTTAATGGTGAATTATACCGCCAATGA", "aa": "MSLVNKDNLLIKEENLKPKKIVFPLIGSIINVQAFKFNGELYRQ", "aa_hexdigest": "a192cefd794e14205ccb8513098c5cb7", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5104.018300000002, "isoelectric_point": 9.601614952087402 }, "id": "OAIKOHHJFB_1104", "locus": "GCA000209735_01090" }, { "type": "cds", "sequence": "contig_1", "start": 606117, "stop": 606503, "strand": "+", "frame": 3, "gene": null, "product": "DUF402 domain-containing protein", "db_xrefs": [ "KEGG:K07586", "SO:0001217", "UniRef:UniRef50_D3VRN0", "UniRef:UniRef90_C4XF90" ], "nt": "ATGCCTAAAAATAGTATGTATAATGCCTTGATTTTATTAAAACCAAAAAGTAATTATGTCTATGTAAATTTAGCTTCAAAACCATATTATGAAGATAATACAATCAAATTTGTTGATTTTGATTTAGATGTTAAAAGCTATCCTAAAAAAGATTTTACAATTGTAGATAAAGAAGAATTTCGTTACAATTCTAGACGCCTCAAATATCCTAAAAAACTGATAAATATGATTTACGATAATTTAGAGGAAATAGTTAATAAACATAATAATGGTGAGTATTTCTTTAATCCAGATGTAATTGATTATTACATCAATCAAGCTAAAAAAGACAAATCAATTTATCGTGATTTTAGAGTTAAAAAAGCAAGTAGTTATAGTTTTAAATGA", "aa": "MPKNSMYNALILLKPKSNYVYVNLASKPYYEDNTIKFVDFDLDVKSYPKKDFTIVDKEEFRYNSRRLKYPKKLINMIYDNLEEIVNKHNNGEYFFNPDVIDYYINQAKKDKSIYRDFRVKKASSYSFK", "aa_hexdigest": "44b38b8bc1d95021df16e688c3f81a6c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 605826, "stop": 606521, "observations": { "mutation": [ 605862, 605997, 606087, 606111, 606501 ], "stop": [ 605862, 605997, 606087, 606111, 606501 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF90", "query_cov": 1.0, "subject_cov": 0.5541125541125541, "identity": 1.0, "score": 259.0, "evalue": 3.2e-86, "gene_start": 1, "gene_end": 128, "reference_start": 98, "reference_end": 225, "reference_sequence": "MKNMEYILKGGKWMSLVNKDNLLIKEENLKPKKIVFPLIGSIINVQAFKFNGELYRQWNGVKVLRNTPQHYVLFMYKTKVAETDKKGWVYREYVLWFMPKNSMYNALILLKPKSNYVYVNLASKPYYEDNTIKFVDFDLDVKSYPKKDFTIVDKEEFRYNSRRLKYPKKLINMIYDNLEEIVNKHNNGEYFFNPDVIDYYINQAKKDKSIYRDFRVKKASSYSFKWRNKNK" }, "paralog": false, "identity": 0.974025974025974, "score": 451.0, "evalue": 4.15e-164, "description": "Internal stop codon at 605862, 605997, 606087, 606111, 606501. Nonsense mutation around 605862, 605997, 606087, 606111, 606501.", "psc": { "uniref90_id": "UniRef90_C4XF90", "product": "DUF402 domain-containing protein", "uniref50_id": "UniRef50_D3VRN0", "kegg_orthology_id": "K07586", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF90", "UniRef:UniRef50_D3VRN0", "KEGG:K07586" ] }, "pscc": { "uniref50_id": "UniRef50_D3VRN0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D3VRN0" ], "product": "DUF402 domain-containing protein" } }, "id": "OAIKOHHJFB_1105", "locus": "GCA000209735_01091" }, { "type": "cds", "sequence": "contig_1", "start": 606830, "stop": 607033, "strand": "-", "frame": 1, "gene": null, "product": "Mutator family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFB3", "UniRef:UniRef90_C4XFB3" ], "nt": "ATGTTTGATTATTCTCTTGAAATAAGAACGCTCATTTATACCACTAATTCAATCGAAAATTTAAATAGCGTATTTAGAAAATATACAAAAACAAAAACGGTTTTCCCATCTGATGATAGTCTTTTAAAAATTCTTTTTCTAGCATCTCAACAAATCATAAAAAATGAAGTAATTCAAGAATTAGAAATTGATCTAGCATTTTAA", "aa": "MFDYSLEIRTLIYTTNSIENLNSVFRKYTKTKTVFPSDDSLLKILFLASQQIIKNEVIQELEIDLAF", "aa_hexdigest": "2117f64c331567bfe1a192fe98a2620d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 606792, "stop": 607186, "observations": { "deletion": [ 606874 ], "mutation": [ 607051, 607063, 607096 ], "stop": [ 606844, 606868, 607051, 607063, 607096 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFB3", "query_cov": 0.8059701492537313, "subject_cov": 0.4122137404580153, "identity": 0.9440000000000001, "score": 99.0, "evalue": 3.02e-25, "gene_start": 1, "gene_end": 54, "reference_start": 52, "reference_end": 105, "reference_sequence": "MKEFCKDLKSVYQSNDAKNAMDNLDIFEDKWGKKYPTSISIWRQNWSEISTMFDYSLEIRTLIYTTNPIENLNSVFRKYAKTKKVFPSDDSLLKILFLASQQIIKKWSNSRIRNWSSILNEFKIIDFENEE" }, "paralog": false, "identity": 0.9236641221374046, "score": 224.0, "evalue": 3.53e-77, "description": "Internal stop codon at 606844, 606868, 607051, 607063, 607096. Frameshift due to deletion around 606874. Nonsense mutation around 607051, 607063, 607096.", "psc": { "uniref90_id": "UniRef90_C4XFB3", "product": "Mutator family transposase", "uniref50_id": "UniRef50_C4XFB3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFB3", "UniRef:UniRef50_C4XFB3" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFB3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFB3" ], "product": "Mutator family transposase" } }, "id": "OAIKOHHJFB_1106", "locus": "GCA000209735_01092" }, { "type": "cds", "sequence": "contig_1", "start": 607094, "stop": 607360, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10551.15" ], "nt": "ATGTCTGTCCTAAATGCTCTTAAAAATAGAGGAATTAGTAAAATTAATATTATTTGTTCAGATAATTTAAAAGGTATACAACAAGCTATAGAAGCCGTTTTTCCTGATAGTAAGCAACAACGTTGCATTGTACATATGATTAGAAATTCTGTTAAATATGTTAACTACAAGGATATGAAAGAATTTTGCAAAGATTTAAAATCTGTATATCAATCTAATGATGCAAAAAATGCAATGGATAATTTGGATATTTTTGAAGATAAATGA", "aa": "MSVLNALKNRGISKINIICSDNLKGIQQAIEAVFPDSKQQRCIVHMIRNSVKYVNYKDMKEFCKDLKSVYQSNDAKNAMDNLDIFEDK", "aa_hexdigest": "b1d2697471a467a119612aa745a6f757", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10551.15", "name": "MULE", "length": 41, "aa_cov": 0.4659090909090909, "hmm_cov": 1.0, "evalue": 7.823055357175163e-09, "score": 32.01397705078125, "start": 9, "stop": 49 } ], "seq_stats": { "molecular_weight": 10129.612099999993, "isoelectric_point": 8.806848716735843 }, "id": "OAIKOHHJFB_1107", "locus": "GCA000209735_01093" }, { "type": "cds", "sequence": "contig_1", "start": 607541, "stop": 608014, "strand": "-", "frame": 1, "gene": null, "product": "Transposase, mutator type", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCS1" ], "nt": "ATGAAAAGAAAACCAATAAATCCTGTTATTGATGAAATAACAGAAAAAATTTTAGAAAATTATAATCCAGTTACTTCTGGTGATCTTAGTATGGCTATGAAAGAAGTATTTCAAAATACAATCCAAAAAATGATGAACAAAGAATTTGATAATTTTATGGGTTATGAAAAAAATGATAATAAAGTTCAAAAAGAAAATTATAGAAACGGTTTTTCTAAGAAAAATGTTAATAGTCAATATGGTCAAATGGAAATTGACATTCCAAGAGATCGTGAAGCGAAATTTGAACCTATAATCATCAAAAAGTATGAACGTGATATTTCTGAATTAGTTGATATGGTATTTGCTTTATATTCTAGAGGAATGTCAACAAGAGATGTAAGTGATTTTATGTTTAGCAAATATGGCGTTAATTATTCTCCAACACAAATAAGCCAATTAACTAATGAAATTGTTGAAGACGCAAGATTATGA", "aa": "MKRKPINPVIDEITEKILENYNPVTSGDLSMAMKEVFQNTIQKMMNKEFDNFMGYEKNDNKVQKENYRNGFSKKNVNSQYGQMEIDIPRDREAKFEPIIIKKYERDISELVDMVFALYSRGMSTRDVSDFMFSKYGVNYSPTQISQLTNEIVEDARL", "aa_hexdigest": "8168e37ca26391bc846580a83a60f9c7", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI00118833E2", "query_cov": 1.0, "subject_cov": 0.8219895287958116, "identity": 0.752, "score": 252.0, "evalue": 2.13e-83, "valid": false, "product": "transposase", "uniref50_id": "UniRef50_A0AB32XCS1" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XCS1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCS1" ], "product": "Transposase, mutator type" }, "genes": [], "id": "OAIKOHHJFB_1108", "locus": "GCA000209735_01094" }, { "type": "cds", "sequence": "contig_1", "start": 608096, "stop": 608239, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATATAACGAATTAAGCTTTACATTTGAATGTAAAGATGGAACTTATTTGTTGAAACCAGATGAAGATCCTTATTTTGCAAAAAATGGTGAATTTTCATCAAGAAATTATTTAACTTACAATATTCAAAAAATTAAATAA", "aa": "MKYNELSFTFECKDGTYLLKPDEDPYFAKNGEFSSRNYLTYNIQKIK", "aa_hexdigest": "80eb07601e6a0004a3c6edc11ee5f710", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5651.2928, "isoelectric_point": 6.104871559143065 }, "id": "OAIKOHHJFB_1109", "locus": "GCA000209735_01095" }, { "type": "cds", "sequence": "contig_1", "start": 608294, "stop": 608536, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTATAAAAATAAATAATTTAACAGCTTCAACGTTAAATCCAATTTATAATATCACACTCGGCAAGGAATATTTTGATGCAAGTCATTACAAAGATATTCTGGACGTTAACACATTAAATTTTACATTTGCTGAAAAAAGTTTAATGGGCTTTAAAACTGTTAGATGCTTAAATGATAATTTTGATGTATTTAAATCGTTAAATATGACAAAAGTAAATCACGAAGGAAAAGAAAAGTGA", "aa": "MTIKINNLTASTLNPIYNITLGKEYFDASHYKDILDVNTLNFTFAEKSLMGFKTVRCLNDNFDVFKSLNMTKVNHEGKEK", "aa_hexdigest": "73d55457139a8fe0920ec00f1f4aa43b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9210.458599999998, "isoelectric_point": 7.853490257263184 }, "id": "OAIKOHHJFB_1110", "locus": "GCA000209735_01096" }, { "type": "cds", "sequence": "contig_1", "start": 608714, "stop": 609382, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCTTGGAATAAAAACATCTATTTTTATTTAAATAATTTTATAGACGAAGAAAAAGATATTATTGTTGATAAAAATATATTTAATATGGAAAGTTATGAACAATTTAATAATGTAAAAATAAGAAATCTATATTTAAGAAATTTTAAATATAGTTTTAATACAGGTGGTTTAAATTTAGATTATTTAAATATTGCAAAAAAATTAAAAGTAAAAAAACTTATAATTGATAAGGATTTATCATTTAAAACATCTTTCAAATTATCCTTAAAAAACACAGATTTCGAGGACATAGAAGGTGCAGAATATTTAATTAAAAACTTATATGTTCCGGAATTAAGTGGAGGAAACTCTGATTTATTTATAAAATTATTGAAAAATAATAAAATTCCTAAATCTTTCAAGATTAAATTAAAAGATTATTCGTTTCCACCAGAAGAAAATGATTTATACGTTAATTGTCTTTATCGTTTATTTAATTTAAATTTAGATTTAGTAGATTTATCCGATTCTGATCCTGTAAATATTTTATATTTTGGAAACCCTAAAAATAGTCAAACCGATAATGATGCATTTAACTGTTATACAGATAAAGTAATTTATCCTAAAAAAGTTGAAAAGGTTGAGGGGGCAATAGCTGCGCATAGATTTAAAAATATTAAATTTTGA", "aa": "MSWNKNIYFYLNNFIDEEKDIIVDKNIFNMESYEQFNNVKIRNLYLRNFKYSFNTGGLNLDYLNIAKKLKVKKLIIDKDLSFKTSFKLSLKNTDFEDIEGAEYLIKNLYVPELSGGNSDLFIKLLKNNKIPKSFKIKLKDYSFPPEENDLYVNCLYRLFNLNLDLVDLSDSDPVNILYFGNPKNSQTDNDAFNCYTDKVIYPKKVEKVEGAIAAHRFKNIKF", "aa_hexdigest": "0d24f8feb620baa6388e230457778c91", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26123.635300000027, "isoelectric_point": 8.557742881774903 }, "id": "OAIKOHHJFB_1111", "locus": "GCA000209735_01097" }, { "type": "cds", "sequence": "contig_1", "start": 609501, "stop": 609809, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATCAAATACAAATTCGCAATTATTTAACAAAGAAATTCTAGAAAATTCTCTTTTTCAATCAAAATTATCATTTGAATTCATAGTTAAAAAGTTTGACGGCATTACTATTTTTTTGAATTTTTTTATATCTTTTTTGTTTTTTCTTAATTCGATAGCTTTTTCTATTTCTGATTTAATTTTTTTTAAATTTTCGTCTTCACTTAATTTAAAATTTCTATTTGAATATTTTATTTTAGCTTCTTCTTCATTGTTGTTTATTCAATTTAATTCACTTATCAAATCATCTTTAGATGAAAATAAATAA", "aa": "MESNTNSQLFNKEILENSLFQSKLSFEFIVKKFDGITIFLNFFISFLFFLNSIAFSISDLIFFKFSSSLNLKFLFEYFILASSSLLFIQFNSLIKSSLDENK", "aa_hexdigest": "f2c7cd8557c5ee9728189e2de00d6607", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11935.688599999981, "isoelectric_point": 5.25041103363037 }, "id": "OAIKOHHJFB_1112", "locus": "GCA000209735_01098" }, { "type": "cds", "sequence": "contig_1", "start": 609920, "stop": 610198, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATTATTAAAATAATAAGAAATTTAAGTATTTTATCACCAATACCAATGGTTTTGAATTCATGTTCAAATACCGATAAGCCTAAAAAACAAGAGGAACAAGAGGAAAAAAAAGTAAAAGAATATCATGTTCCGACTTCTTGCGTAGATTATGACAAAGATGAATTAAAAAATATATTAGAAAAAAATACTAAATTAGTAAAAAATGTTAATACAAATAAAAAAGAAAAATATATTGATTATGATTTCGTTTATGACGTTATACAAAAATGA", "aa": "MKKIIKIIRNLSILSPIPMVLNSCSNTDKPKKQEEQEEKKVKEYHVPTSCVDYDKDELKNILEKNTKLVKNVNTNKKEKYIDYDFVYDVIQK", "aa_hexdigest": "23f0fd9532dbe95eeb41542216cbed66", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10904.566399999992, "isoelectric_point": 8.985297203063965 }, "id": "OAIKOHHJFB_1113", "locus": "GCA000209735_01099" }, { "type": "cds", "sequence": "contig_1", "start": 610302, "stop": 610574, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13304.12" ], "nt": "ATGATCGAACCTAAGGTTTTACTTTTAGACGAACCTGAAGCAAATTTGGATCCTTATGCAAGAAATCAACTTTATCAAAGACTTCAAGCTTTAAAGCAAAAGGGCGTGTCAATAATTTTAGCATCTCACGTTATAGATTCAAATAATGAAATAATTGATTACATTACTATTTTGAACGAAAAGGAAATAGTATTTGAATCAAAAATAAACTCAGTAAAAGAATCATCTAGCATTTATGAAAATTTTAATAAAGAAATATATGATAAGATTTAA", "aa": "MIEPKVLLLDEPEANLDPYARNQLYQRLQALKQKGVSIILASHVIDSNNEIIDYITILNEKEIVFESKINSVKESSSIYENFNKEIYDKI", "aa_hexdigest": "2fc80ea22c636a358b4386f44b814cba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13304.12", "name": "AAA_21", "length": 40, "aa_cov": 0.4444444444444444, "hmm_cov": 1.0, "evalue": 1.1868253086788488e-09, "score": 34.73923873901367, "start": 4, "stop": 43 } ], "seq_stats": { "molecular_weight": 10441.781599999993, "isoelectric_point": 4.774384498596191 }, "id": "OAIKOHHJFB_1114", "locus": "GCA000209735_01100" }, { "type": "cds", "sequence": "contig_1", "start": 611169, "stop": 611639, "strand": "+", "frame": 3, "gene": null, "product": "MAGa4850 family ICE element protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF94", "UniRef:UniRef90_C4XF94" ], "nt": "ATGAGTGTATACGGCGAACTAAGGTTAATTGCAAATGAAGGAAGCGAAAACGAAGTTAGAAAGTTTGAAGCTAACTTGGAATGGAGAAAAAATTATTTTGGCAAGAAGGTTTATGACAAGTTAAGCCAAGATACAGTTAGTGAAATTTTAAAAACAAAGATAGTACTCGGTGAAAGCTACTATAAGATTTTGAGAACTGAAAAAGTTGCTTTTGAAGTGTATGTCTGTTTAAGGTTTCTTGGGGCTAAAAAACGCTATGTAAATTTTTATGAATTAGTGGCTTTTGGCTTCAAGAAAACAAGTTTGCAAAGAGCGGTAAAGTTTTTAACAGATATAGGGCTAATTTTAAAAGTTAGAAACGCTGTGAAAATTAAGAAGTTTAAGCTAACTAATGACGATAGAAAGTTCATTGTTATTAGTGGTTATAAAGACTGGAAGATTTTCTTGTTGTTTGGATTAGCTAATTTATGA", "aa": "MSVYGELRLIANEGSENEVRKFEANLEWRKNYFGKKVYDKLSQDTVSEILKTKIVLGESYYKILRTEKVAFEVYVCLRFLGAKKRYVNFYELVAFGFKKTSLQRAVKFLTDIGLILKVRNAVKIKKFKLTNDDRKFIVISGYKDWKIFLLFGLANL", "aa_hexdigest": "adc8cbbec5672ec1b725519e74a278c1", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 611097, "stop": 611888, "observations": { "mutation": [ 611637, 611658 ], "stop": [ 611637, 611658 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF94", "query_cov": 1.0, "subject_cov": 0.5931558935361216, "identity": 1.0, "score": 304.0, "evalue": 6.56e-103, "gene_start": 1, "gene_end": 156, "reference_start": 25, "reference_end": 180, "reference_sequence": "MLPVRPVVYIIPQSLLYVQKKGEIMSVYGELRLIANEGSENEVRKFEANLEWRKNYFGKKVYDKLSQDTVSEILKTKIVLGESYYKILRTEKVAFEVYVCLRFLGAKKRYVNFYELVAFGFKKTSLQRAVKFLTDIGLILKVRNAVKIKKFKLTNDDRKFIVISGYKDWKIFLLFGLANLWAYKTLVWKSKELGTKKFVKSRKMKVIVQNNFLGLKGSTAYRYLKNICLVLGLRTDELFIIQRSVDNLQHLSFKTQRYLVIRI" }, "paralog": false, "identity": 0.9885931558935361, "score": 506.0, "evalue": 6.6e-185, "description": "Internal stop codon at 611637, 611658. Nonsense mutation around 611637, 611658.", "psc": { "uniref90_id": "UniRef90_C4XF94", "product": "MAGa4850 family ICE element protein", "uniref50_id": "UniRef50_C4XF94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF94", "UniRef:UniRef50_C4XF94" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF94" ] } }, "id": "OAIKOHHJFB_1115", "locus": "GCA000209735_01101" }, { "type": "cds", "sequence": "contig_1", "start": 612119, "stop": 612310, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013526872.1", "SO:0001217", "UniParc:UPI00000C0690", "UniRef:UniRef100_C4XF95", "UniRef:UniRef50_C4XF95", "UniRef:UniRef90_C4XF95" ], "nt": "ATGAAATACAAAGAAGGACAATTAATTTTAGTTAACTATAAGTTTGCTCAAAAGTTTTTATCTTCATCAGTTGGAGATGATTTTCTCTATTTGGGATTACAAAATCGTAAAAACTTAGCTAAAAGACTTTCTCAAATTATGAATATACCAAAACTTACTCCTGAGTTTCATTGCATAGCTAAAGGGAGCTAG", "aa": "MKYKEGQLILVNYKFAQKFLSSSVGDDFLYLGLQNRKNLAKRLSQIMNIPKLTPEFHCIAKGS", "aa_hexdigest": "4e10fe405d634def018d3530d31e48fc", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C0690", "ncbi_nrp_id": "WP_013526872.1", "uniref100_id": "UniRef100_C4XF95", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C0690", "RefSeq:WP_013526872.1", "UniRef:UniRef100_C4XF95" ] }, "ips": { "uniref100_id": "UniRef100_C4XF95", "uniref90_id": "UniRef90_C4XF95" }, "psc": { "uniref90_id": "UniRef90_C4XF95", "uniref50_id": "UniRef50_C4XF95" }, "pscc": { "uniref50_id": "UniRef50_C4XF95", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF95" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7235.454599999999, "isoelectric_point": 9.779418754577634 }, "id": "OAIKOHHJFB_1116", "locus": "GCA000209735_01102" }, { "type": "cds", "sequence": "contig_1", "start": 612571, "stop": 612744, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTAGCCAATATTCTGGTGTTTATGATATGCTAAACGATTCTAGTGCTGATGTAAATATCGATAACGTTAAGGAAGAATTAAAGAGTATTGATGAACAAACATTTTATGAAGGCATTTTAAACTTAGGTAATTTAGGTTTAGAACATAATGTTTTAACTGCTAATGATTGA", "aa": "MGSQYSGVYDMLNDSSADVNIDNVKEELKSIDEQTFYEGILNLGNLGLEHNVLTAND", "aa_hexdigest": "2e7232a416963aea6061be918ee46d1c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6294.762199999999, "isoelectric_point": 4.0500284194946286 }, "id": "OAIKOHHJFB_1117", "locus": "GCA000209735_01103" }, { "type": "cds", "sequence": "contig_1", "start": 612862, "stop": 613002, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTTGAAAAGACTAAAAAAAGAGCAAAAAGAAAATTAAACATCGACGATATAAAAATGCTAAGAATGAAGATTACAAACAAGCTTATTAAAAACTTTGATTATCAAAATCTTAAGGATTTAACAAGTAAGTTATGA", "aa": "MFFEKTKKRAKRKLNIDDIKMLRMKITNKLIKNFDYQNLKDLTSKL", "aa_hexdigest": "3818316b54ed4b32cdd1dece0c67879e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5661.7964, "isoelectric_point": 10.291749000549316 }, "id": "OAIKOHHJFB_1118", "locus": "GCA000209735_01104" }, { "type": "cds", "sequence": "contig_1", "start": 613108, "stop": 613449, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGAATATGTTCAATAAGTCTTATAAACTTGCTTCACCAGAAGTTAAAGAAGCGATAAACAAGATAGAAACATTTTTATATAAATATGATGATGATTTTAAAGGAAAGATGGATAACTATCAAAAGGCATTTGATTTAGTTATGCAACTAAAGGATGAAACGAATTCAACGTTTTTAAAAAAGAGAATCAATAATGTTTTGAGAACTGAAAAAGATGAGTTTTCTAGACAGCTAGGGAACAAGATAATAAAAGCAATTCTAAAATCAAGAAATGCTAAGAGTGCTAAAAGCTTTACAAAATTATTCTTTTATAACTTGAATGTAAATGATGATTCTTGA", "aa": "MENMFNKSYKLASPEVKEAINKIETFLYKYDDDFKGKMDNYQKAFDLVMQLKDETNSTFLKKRINNVLRTEKDEFSRQLGNKIIKAILKSRNAKSAKSFTKLFFYNLNVNDDS", "aa_hexdigest": "ca03355bf950c1fafc7488b9f7c761da", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13355.14579999998, "isoelectric_point": 9.442893791198731 }, "id": "OAIKOHHJFB_1119", "locus": "GCA000209735_01105" }, { "type": "cds", "sequence": "contig_1", "start": 613543, "stop": 613755, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF97", "UniRef:UniRef90_C4XF97" ], "nt": "ATGGGAAACAATATATCTAAGATAGTTAAAAAAATTATAGAAACGGAAGATTGGAATGATGTTATAACGGGTATCAATTTACTTCATTATAAAAAGATTTTGAAAAACAATATGCCATTAAGAAACGTAGCACTTGTATTGTTTTCAATATCAATTTTAGTTTTCTTCATATTTGCTAGCCTAGTGAATGTATTAAATATAAGTTTTGCTTGA", "aa": "MGNNISKIVKKIIETEDWNDVITGINLLHYKKILKNNMPLRNVALVLFSISILVFFIFASLVNVLNISFA", "aa_hexdigest": "0635f0269fe1de7e3229df2928cd1cab", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 613540, "stop": 614010, "observations": { "mutation": [ 613753 ], "stop": [ 613753 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF97", "query_cov": 1.0, "subject_cov": 0.44871794871794873, "identity": 1.0, "score": 131.0, "evalue": 1.21e-37, "gene_start": 1, "gene_end": 70, "reference_start": 2, "reference_end": 71, "reference_sequence": "MMGNNISKIVKKIIETEDWNDVITGINLLHYKKILKNNMPLRNVALVLFSISILVFFIFASLVNVLNISFAWNISITVSSSLLMVVFLCMMLLIKPIRMKYKCDQKFIEHLGLSVEGYCQIIEECLCIAKAIPMQKLAKRIIVQKLDIFMAEGGGQ" }, "paralog": false, "identity": 0.9871794871794872, "score": 300.0, "evalue": 7.42e-107, "description": "Internal stop codon at 613753. Nonsense mutation around 613753.", "psc": { "uniref90_id": "UniRef90_C4XF97", "uniref50_id": "UniRef50_C4XF97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF97", "UniRef:UniRef50_C4XF97" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF97" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7962.459199999995, "isoelectric_point": 9.601679420471193 }, "id": "OAIKOHHJFB_1120", "locus": "GCA000209735_01106" }, { "type": "cds", "sequence": "contig_1", "start": 613789, "stop": 614010, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF97", "UniRef:UniRef90_C4XF97" ], "nt": "ATGGTTGTATTCCTTTGCATGATGTTATTAATAAAGCCTATAAGAATGAAATATAAATGCGACCAGAAGTTTATTGAACATTTAGGCTTAAGCGTAGAAGGCTATTGTCAAATAATTGAAGAATGCCTTTGCATAGCTAAAGCAATTCCAATGCAAAAGTTAGCTAAAAGAATTATTGTGCAAAAGCTTGATATTTTTATGGCTGAAGGCGGAGGTCAATAA", "aa": "MVVFLCMMLLIKPIRMKYKCDQKFIEHLGLSVEGYCQIIEECLCIAKAIPMQKLAKRIIVQKLDIFMAEGGGQ", "aa_hexdigest": "4e375f5225f369e001680770108e444f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 613540, "stop": 614010, "observations": { "mutation": [ 613753 ], "stop": [ 613753 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XF97", "query_cov": 1.0, "subject_cov": 0.46794871794871795, "identity": 1.0, "score": 147.0, "evalue": 3.96e-44, "gene_start": 1, "gene_end": 73, "reference_start": 84, "reference_end": 156, "reference_sequence": "MMGNNISKIVKKIIETEDWNDVITGINLLHYKKILKNNMPLRNVALVLFSISILVFFIFASLVNVLNISFAWNISITVSSSLLMVVFLCMMLLIKPIRMKYKCDQKFIEHLGLSVEGYCQIIEECLCIAKAIPMQKLAKRIIVQKLDIFMAEGGGQ" }, "paralog": false, "identity": 0.9871794871794872, "score": 300.0, "evalue": 8.3e-107, "description": "Internal stop codon at 613753. Nonsense mutation around 613753.", "psc": { "uniref90_id": "UniRef90_C4XF97", "uniref50_id": "UniRef50_C4XF97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XF97", "UniRef:UniRef50_C4XF97" ] }, "pscc": { "uniref50_id": "UniRef50_C4XF97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF97" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8364.290099999997, "isoelectric_point": 8.664953804016115 }, "id": "OAIKOHHJFB_1121", "locus": "GCA000209735_01107" }, { "type": "cds", "sequence": "contig_1", "start": 614010, "stop": 614306, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGATAAGAAAAAGAAAATATTAAAAATATCTTTAAGTGCTGTTGCAATACTTCTTGCAGCAATTATCATCTTCCTAGCTCTAGCCATTATATTTAATGTTAAGCGTTTGAAAAGTTTTAAGATGGTTTCAATTAAAGACTTTTTTGCACAGACAAAAGCAAGCATTTATGCATTTCCAAAACAGTTTTTAATTTCATACGCATTTTTATTAATTGCATCGATAGCGGGTATAAGTTATTTATTCTATAAAAATAAACTTAAAACATCATTATTAAAACCTAATAAGTATTGA", "aa": "MGDKKKKILKISLSAVAILLAAIIIFLALAIIFNVKRLKSFKMVSIKDFFAQTKASIYAFPKQFLISYAFLLIASIAGISYLFYKNKLKTSLLKPNKY", "aa_hexdigest": "a7ee8fb1bcb6146dfc81f181ec136612", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11069.498099999988, "isoelectric_point": 10.299291801452636 }, "id": "OAIKOHHJFB_1122", "locus": "GCA000209735_01108" }, { "type": "cds", "sequence": "contig_1", "start": 614445, "stop": 614747, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATGAACCTGAAAGTGATATTAACTCAGTTGTTTTGGGGGGTGTAGGTTCAGGTAAAACACAACGTGTACTATTGCCTAATATTATTTATAATTCACATCTAAGATATAAATATAGAGCTAATTTCTGTATTCCGGATCCAAAAAAAGAAATTATCAAAATGGTTGGTAAAAAGCTTGAAAAGCAAGGATACAAAATATATGCGGTAGATTTTAGCGATACAAAGAAAAGTGTTGGTTGGAATCCATTATCATTTGCTTATAAACTAGCTCATAGAGTGCCGGAAGGGCATCCTAAATGA", "aa": "MNEPESDINSVVLGGVGSGKTQRVLLPNIIYNSHLRYKYRANFCIPDPKKEIIKMVGKKLEKQGYKIYAVDFSDTKKSVGWNPLSFAYKLAHRVPEGHPK", "aa_hexdigest": "6390e01b408d03e3933cb69dff4b3dd5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11349.04659999999, "isoelectric_point": 9.68065319061279 }, "id": "OAIKOHHJFB_1123", "locus": "GCA000209735_01109" }, { "type": "cds", "sequence": "contig_1", "start": 615273, "stop": 615857, "strand": "+", "frame": 3, "gene": "traD", "product": "TraM recognition site of TraD and TraG", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XBH1", "UniRef:UniRef90_A0AB32XBH1" ], "nt": "ATGCTTATTGACCAAATATATCAAGCTTTAATTGATTGCGCTAATAGTAAGGAAGACTTGAAACTTGAACGGAAGTTCTTCTTCATGTTAGATGAAGCGGGGAACCTGCCTCCAATATTGAATCTAGATAATAAGGTCACAATATCGCGTAGTAGAAATGTATTTTTCCAACTTGTATTGCAAGATTACAATCAGCTTAAAAAATACAATACAAGCACTAATCATAATGTGGATAGCATTATTCGTAGTAATTTGCAATTTACTTATTTCTTGAACTCTATTGATGAAAAAACTTTAAGCGATTTATCTAATTCTTTAGGCAAAAAAGAAGTTATTAAAAAATCAACTTCAAAATCATCTTCAACATCAGCTAATGGTGGTTCATCTTCAGAAAGTGAATCGGTGGAAGAAAAGCCTTTAATGAGTGTGGCGGAAATTAAAGCGAAGAATAAAGAATATGCCATTATTCAAAAAATTGGTTTTCTTCCTATGATAGTAAAAACAAAATTAGCTTACAAATATTTTAGTAATGATAATTATGTTTTTGAATGTGACGAAACAGAAAACACTGTGAATGAATCTTGA", "aa": "MLIDQIYQALIDCANSKEDLKLERKFFFMLDEAGNLPPILNLDNKVTISRSRNVFFQLVLQDYNQLKKYNTSTNHNVDSIIRSNLQFTYFLNSIDEKTLSDLSNSLGKKEVIKKSTSKSSSTSANGGSSSESESVEEKPLMSVAEIKAKNKEYAIIQKIGFLPMIVKTKLAYKYFSNDNYVFECDETENTVNES", "aa_hexdigest": "f918f8a0bf6604f81688e687aaff7c58", "start_type": "ATG", "rbs_motif": null, "genes": [ "traD" ], "truncated": "both", "pseudogene": { "start": 614991, "stop": 616160, "observations": { "mutation": [ 615036, 615855, 616095 ], "stop": [ 615036, 615855, 616095 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XBH1", "query_cov": 1.0, "subject_cov": 0.470873786407767, "identity": 1.0, "score": 343.0, "evalue": 1.9e-115, "gene_start": 1, "gene_end": 194, "reference_start": 95, "reference_end": 288, "reference_sequence": "MSICYINAETNKRYYWAELFKNVKTIANIVPETLTGNLSHTTSALKNFTEDEFIKRLTRETKSFDLHGIVSSNEPYVIFIHYPDHKPSNHFLVSMLIDQIYQALIDCANSKEDLKLERKFFFMLDEAGNLPPILNLDNKVTISRSRNVFFQLVLQDYNQLKKYNTSTNHNVDSIIRSNLQFTYFLNSIDEKTLSDLSNSLGKKEVIKKSTSKSSSTSTNGGSSSESESVEEKPLMSVAEIKAKNKEYAIIQKIGFLPMIVKTKLAYKYFSNDNYVFECDETENTVNESWNYQDLPDVELPVRNPIKNYDQLNDSSRAKKAREESLNKELNDKKVQKKLILEMQNARGSIKELSEYKDKISEWPKLALAWQKILSFQQKYKNKLSSELVNDFTKSINICLDQMNDIKTAGAKT" }, "paralog": false, "identity": 0.9897172236503856, "score": 728.0, "evalue": 1.35e-269, "description": "Internal stop codon at 615036, 615855, 616095. Nonsense mutation around 615036, 615855, 616095.", "psc": { "uniref90_id": "UniRef90_A0AB32XBH1", "product": "TraM recognition site of TraD and TraG", "uniref50_id": "UniRef50_A0AB32XBH1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XBH1", "UniRef:UniRef50_A0AB32XBH1" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB32XBH1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XBH1" ] } }, "id": "OAIKOHHJFB_1124", "locus": "GCA000209735_01110" }, { "type": "cds", "sequence": "contig_1", "start": 615870, "stop": 616097, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAGATGTTGAATTACCGGTTAGAAATCCTATTAAAAACTATGACCAATTGAATGATAGTTCAAGAGCCAAGAAAGCTAGAGAGGAAAGCCTCAATAAAGAACTTAATGATAAGAAAGTTCAAAAGAAACTTATTTTGGAAATGCAAAATGCAAGAGGAAGTATTAAGGAGCTTAGCGAGTATAAAGACAAGATAAGCGAATGGCCTAAATTAGCACTTGCTTGA", "aa": "MPDVELPVRNPIKNYDQLNDSSRAKKAREESLNKELNDKKVQKKLILEMQNARGSIKELSEYKDKISEWPKLALA", "aa_hexdigest": "a7bce82dcd65675848f6e9422130e73d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8724.933399999994, "isoelectric_point": 9.221702766418456 }, "id": "OAIKOHHJFB_1125", "locus": "GCA000209735_01111" }, { "type": "cds", "sequence": "contig_1", "start": 616240, "stop": 616428, "strand": "+", "frame": 1, "gene": null, "product": "ICEF-IA ORF6", "db_xrefs": [ "RefSeq:WP_013526825.1", "SO:0001217", "UniParc:UPI00000C073C", "UniRef:UniRef100_Q8G8G5", "UniRef:UniRef50_Q8G8G5", "UniRef:UniRef90_Q8G8G5" ], "nt": "ATGAAAAGATATTTTTACAGAATTAGAGTCAAAAAGAATCTTGAATCTAAGAAAACAAAATCATATGCAGTTTGCTCTAACTTTAATGAGGCAGACAAGATAGCCAATGAAATTATAGAAAAAGGAGATTATGGTTGGGTGCAAGTTGAAAGAGTAGAAAAGATTAAACATTATTTTAAGGAGGTTTAA", "aa": "MKRYFYRIRVKKNLESKKTKSYAVCSNFNEADKIANEIIEKGDYGWVQVERVEKIKHYFKEV", "aa_hexdigest": "536d7d5d695db8bc2e435e625cfadd0d", "start_type": "ATG", "rbs_motif": "AGxAG", "ups": { "uniparc_id": "UPI00000C073C", "ncbi_nrp_id": "WP_013526825.1", "uniref100_id": "UniRef100_Q8G8G5", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C073C", "RefSeq:WP_013526825.1", "UniRef:UniRef100_Q8G8G5" ] }, "ips": { "uniref100_id": "UniRef100_Q8G8G5", "uniref90_id": "UniRef90_Q8G8G5" }, "psc": { "uniref90_id": "UniRef90_Q8G8G5", "product": "ICEF-IA ORF6", "uniref50_id": "UniRef50_Q8G8G5" }, "pscc": { "uniref50_id": "UniRef50_Q8G8G5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8G8G5" ], "product": "ICEF-IA ORF6" }, "genes": [], "id": "OAIKOHHJFB_1126", "locus": "GCA000209735_01112" }, { "type": "cds", "sequence": "contig_1", "start": 616430, "stop": 617377, "strand": "+", "frame": 2, "gene": null, "product": "MORN repeat protein", "db_xrefs": [ "RefSeq:WP_013526824.1", "SO:0001217", "UniParc:UPI00000C02A4", "UniRef:UniRef100_C4XF99", "UniRef:UniRef50_C4XF99", "UniRef:UniRef90_C4XF99" ], "nt": "ATGCAAGTATTTAATGCATTAGAAAACGAAGAAATTATTAATCGTAGAAACTATGAAAAGAACGGAACTAATTTTGAATTAATTAGTACAAAAAGTGGTTTGACTTTAGCACATAATGTTTTAAATGATAATAGTCAAGAATACTATGAAATGAACGAAGGAGCTTTTAGTGGACGCTATTACAAAATTGCTGAAAATAATCAGCTCGTTGGCGTTGCGAATTACCAAAATGATGAATTAAATGGTGAAGCAACAATTTATAACACTAAGGGCGATATTCTTTATAAAGCACATTTTGAAGATGGTTTATTAAATGGGGCTGTTGAAGAATATAAAGACGGCGCGCTAGTTAAAAGCACAATGTATGAAAATGGATTTAAAACTGGCGAAGAAGTTGTATATGATGCTAAAGGAGAAGTAGAAGGAAAAACTAATTTTATTACTAATAATGAAGTAAAAGAAAAAGAGGTAAAAGAATATAAAGAAGCTGATAAAGAAAAGAAAGCGGAAATAAGAAACAAGAACTTTAAGGAAGTTTTGAAGAAATTACCTGCTCTTGCATTAAAAGGGTTCTTATGTCTTCAAGCATATTTAGTGGGCGGTTTGCCTTTAGTAATAGGCATAACTGCTATTAAATATTTCTTTGATAAAAGAAAAGAAAAAGCTTTAGCTAAAAATGAGATAAAGCCGGAAATTGCAAAGAACTTTAAGTTGAGCGATGGTGTTGCTAAAGAGATAACAAAAACGTTTAATGAAGCAATGAAAGAAATTGTTGTTTCAACTAAAGAGGCTGTGATGAGCCAATACCAAAAGGATTTAGCAAGTGGAAAGATTATAGCTGACGCATATGATAATGCGAATATTGAAAAAGCACAAAATGCAGCTATGAATAAAGAAATTGCTAAAGAAACAAAAATTGAAAATCCAAAGTCAAAGGCTAAAAAATAG", "aa": "MQVFNALENEEIINRRNYEKNGTNFELISTKSGLTLAHNVLNDNSQEYYEMNEGAFSGRYYKIAENNQLVGVANYQNDELNGEATIYNTKGDILYKAHFEDGLLNGAVEEYKDGALVKSTMYENGFKTGEEVVYDAKGEVEGKTNFITNNEVKEKEVKEYKEADKEKKAEIRNKNFKEVLKKLPALALKGFLCLQAYLVGGLPLVIGITAIKYFFDKRKEKALAKNEIKPEIAKNFKLSDGVAKEITKTFNEAMKEIVVSTKEAVMSQYQKDLASGKIIADAYDNANIEKAQNAAMNKEIAKETKIENPKSKAKK", "aa_hexdigest": "0cdba05f663e8efc85d8182828c25163", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI00000C02A4", "ncbi_nrp_id": "WP_013526824.1", "uniref100_id": "UniRef100_C4XF99", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C02A4", "RefSeq:WP_013526824.1", "UniRef:UniRef100_C4XF99" ] }, "ips": { "uniref100_id": "UniRef100_C4XF99", "uniref90_id": "UniRef90_C4XF99" }, "psc": { "uniref90_id": "UniRef90_C4XF99", "product": "MORN repeat protein", "uniref50_id": "UniRef50_C4XF99" }, "pscc": { "uniref50_id": "UniRef50_C4XF99", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XF99" ], "product": "MORN repeat protein" }, "genes": [], "id": "OAIKOHHJFB_1127", "locus": "GCA000209735_01113" }, { "type": "cds", "sequence": "contig_1", "start": 617386, "stop": 617625, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013526869.1", "SO:0001217", "UniParc:UPI00000C02C2", "UniRef:UniRef100_C4XFA0", "UniRef:UniRef50_C4XFA0", "UniRef:UniRef90_C4XFA0" ], "nt": "ATGGAAAAGAAGAAAGGAAAAGGAAAAATTATGCATGAAATTAAAAACAATAAATTTGATGAAATTTTATTAAGTGAAGAATCAAAACAAAAAATAGATCAAGATTTAAAACAAAATGGTATCAAAAGTACCTTAGCTAAAAAATATCTTGAAGAATTCAAAAAAATGGAACCAGAAAAATATGAAAAATTTAGAAAAGAAAGCAATAAAAAAATATGCAATAAGCTTTTTGCTAAATAG", "aa": "MEKKKGKGKIMHEIKNNKFDEILLSEESKQKIDQDLKQNGIKSTLAKKYLEEFKKMEPEKYEKFRKESNKKICNKLFAK", "aa_hexdigest": "9df3ce0167d8cced414b46ed0b0c03a8", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C02C2", "ncbi_nrp_id": "WP_013526869.1", "uniref100_id": "UniRef100_C4XFA0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C02C2", "RefSeq:WP_013526869.1", "UniRef:UniRef100_C4XFA0" ] }, "ips": { "uniref100_id": "UniRef100_C4XFA0", "uniref90_id": "UniRef90_C4XFA0" }, "psc": { "uniref90_id": "UniRef90_C4XFA0", "uniref50_id": "UniRef50_C4XFA0" }, "pscc": { "uniref50_id": "UniRef50_C4XFA0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9481.049399999993, "isoelectric_point": 9.631012535095213 }, "id": "OAIKOHHJFB_1128", "locus": "GCA000209735_01114" }, { "type": "cds", "sequence": "contig_1", "start": 617638, "stop": 618354, "strand": "+", "frame": 1, "gene": null, "product": "Mbov_0392 family ICE element protein", "db_xrefs": [ "RefSeq:WP_013526868.1", "SO:0001217", "UniParc:UPI00000C0686", "UniRef:UniRef100_C4XFA1", "UniRef:UniRef50_C4XFA1", "UniRef:UniRef90_C4XFA1" ], "nt": "ATGATTGAAAAAGTTTTTTGTTTTAAGGATTTGTATTATCTAACTCAATATGCATTAAGTGATATTTCAGATGAAGAAGTTGTAGCTAAATTTAACTATTTAGCAAAAAATATCAAGAATAATACTATGCGGGATAAATATAAAAAACAAGTATTATCTATAGTAGACAAAATGTCTAATGTAGACGATGATATAAATCAAAAGATTTTTGATAAATTGTTTTATGTTGAAGATACAAATGAAAAAAATTTGGAGCAATACATCACTTTATTAAATATATGCAATGTCGAAGATTTAGAAAATATAGAAAATAATAGTTTAGATTTAAAATTCTCATTTAATGATTTTTTTGGTGAAAATGGTTATATAGAAAGTGAATATTTATTTGATTTAGTAGATGAAGATTTAAAACAAAATGGAATAAACAGTAAATATATTTATTCACTTGGTGATTTAAGTAGAATCGATACAGAATATGTTTATATTGATGATTATTTAAATTTTCATCCAGAAGATATTGATGACATAAAAAAAGAATATATAGAAAATTTAGCTTCTTTAAAATTAGATGAAATCATTGATTTAAATAATAAAGAAAGTATTGATGCAAATAATAAGGTTGAAATAACTGATAAAACTGTTAAAGAAGCTCAAAAACATGTTGCTGGTATTGTAGAAAAAGAAAGTGAAACAGCAAAAGTTAAAAATACAAAATAA", "aa": "MIEKVFCFKDLYYLTQYALSDISDEEVVAKFNYLAKNIKNNTMRDKYKKQVLSIVDKMSNVDDDINQKIFDKLFYVEDTNEKNLEQYITLLNICNVEDLENIENNSLDLKFSFNDFFGENGYIESEYLFDLVDEDLKQNGINSKYIYSLGDLSRIDTEYVYIDDYLNFHPEDIDDIKKEYIENLASLKLDEIIDLNNKESIDANNKVEITDKTVKEAQKHVAGIVEKESETAKVKNTK", "aa_hexdigest": "7a9c47a621909a378797adedb1d43586", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00000C0686", "ncbi_nrp_id": "WP_013526868.1", "uniref100_id": "UniRef100_C4XFA1", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C0686", "RefSeq:WP_013526868.1", "UniRef:UniRef100_C4XFA1" ] }, "ips": { "uniref100_id": "UniRef100_C4XFA1", "uniref90_id": "UniRef90_C4XFA1" }, "psc": { "uniref90_id": "UniRef90_C4XFA1", "product": "Mbov_0392 family ICE element protein", "uniref50_id": "UniRef50_C4XFA1" }, "pscc": { "uniref50_id": "UniRef50_C4XFA1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA1" ] }, "genes": [], "id": "OAIKOHHJFB_1129", "locus": "GCA000209735_01115" }, { "type": "cds", "sequence": "contig_1", "start": 618364, "stop": 618915, "strand": "+", "frame": 1, "gene": null, "product": "Single-stranded DNA-binding protein", "db_xrefs": [ "RefSeq:WP_013526867.1", "SO:0001217", "UniParc:UPI00000C02C3", "UniRef:UniRef100_C4XFA2", "UniRef:UniRef50_C4XFA2", "UniRef:UniRef90_C4XFA2" ], "nt": "ATGAATAAAGTTATATTAACGGGTAGAGTAGTAAGTGAAGACTACTTTAAAGCTAAAACAACAAGTGGTAAAGCTGTTTTAGGGTTTGTGATTGCTGTAAATAATGAATATGGTGGGGAACCTGATTTTCTAAATATTACAGCTTTTGGTTTGCAAGCAAGCAATTTTGAAAAGTACAATAAAAAAGGCGACTTAGTCGAAGTTATTGGAAAGATAAAGAGAAATGCTTATGTGGATAGAGATAATCATAAGCAATACAAAATGGAAATTGTTGCTGACAATATTATTTATAGTGCTAAAAAAGTTGATAGAAACGCTGAAGGTAATGATGAAGATATTATTTATAAAGATGAAACAAAATTACCTAAGGAGGAAAAAGAAAAGGTTGTTAAAGCGAGACTAAAGAAAAAGGATGAAATGATGGAACCTTTTAAACAATATATTGGTATGGATCCATTGTGTCAAAGCGTCTATGATAAATACGAAGAAGAAGCTTTTAACAAAGATGTTGAAGTAAAAGATGAAACTAAAAAACACGGTCAAACATTGTAA", "aa": "MNKVILTGRVVSEDYFKAKTTSGKAVLGFVIAVNNEYGGEPDFLNITAFGLQASNFEKYNKKGDLVEVIGKIKRNAYVDRDNHKQYKMEIVADNIIYSAKKVDRNAEGNDEDIIYKDETKLPKEEKEKVVKARLKKKDEMMEPFKQYIGMDPLCQSVYDKYEEEAFNKDVEVKDETKKHGQTL", "aa_hexdigest": "7876c7aa53c01bbcd9e8e8b7b6e1a631", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000C02C3", "ncbi_nrp_id": "WP_013526867.1", "uniref100_id": "UniRef100_C4XFA2", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000C02C3", "RefSeq:WP_013526867.1", "UniRef:UniRef100_C4XFA2" ] }, "ips": { "uniref100_id": "UniRef100_C4XFA2", "uniref90_id": "UniRef90_C4XFA2" }, "psc": { "uniref90_id": "UniRef90_C4XFA2", "product": "Single-stranded DNA-binding protein", "uniref50_id": "UniRef50_C4XFA2" }, "pscc": { "uniref50_id": "UniRef50_C4XFA2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA2" ], "product": "Single-stranded DNA-binding protein" }, "genes": [], "id": "OAIKOHHJFB_1130", "locus": "GCA000209735_01116" }, { "type": "cds", "sequence": "contig_1", "start": 618915, "stop": 619112, "strand": "+", "frame": 3, "gene": null, "product": "ICEF-IA ORF13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8G945", "UniRef:UniRef90_Q8G945" ], "nt": "ATGAAAAACTATTTGAAATTTAAGTTTTTTATTATTTACATAGTATTCTGGATATTAATGCTGATGATTTTAAGTTTATCTTTTGTGGCCGTTAATGGAATGTATGTTGATAAAGAATTGACAGAAACTAAGTATGGAGTTATCGAAGGAATAATTTTATTTCTTGGAATGGCGACTTTCATATATCCAGTATATTGA", "aa": "MKNYLKFKFFIIYIVFWILMLMILSLSFVAVNGMYVDKELTETKYGVIEGIILFLGMATFIYPVY", "aa_hexdigest": "37a7ed9202ae80cc53604b2722443644", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 618915, "stop": 619169, "observations": { "mutation": [ 619110, 619134 ], "stop": [ 619110, 619134 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q8G945", "query_cov": 1.0, "subject_cov": 0.7738095238095238, "identity": 1.0, "score": 122.0, "evalue": 3.68e-35, "gene_start": 1, "gene_end": 65, "reference_start": 1, "reference_end": 65, "reference_sequence": "MKNYLKFKFFIIYIVFWILMLMILSLSFVAVNGMYVDKELTETKYGVIEGIILFLGMATFIYPVYWVKKRIEYWKQSKKNSDLH" }, "paralog": false, "identity": 0.9761904761904762, "score": 162.0, "evalue": 1.27e-53, "description": "Internal stop codon at 619110, 619134. Nonsense mutation around 619110, 619134.", "psc": { "uniref90_id": "UniRef90_Q8G945", "product": "ICEF-IA ORF13", "uniref50_id": "UniRef50_Q8G945", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q8G945", "UniRef:UniRef50_Q8G945" ] }, "pscc": { "uniref50_id": "UniRef50_Q8G945", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8G945" ], "product": "ICEF-IA ORF13" } }, "id": "OAIKOHHJFB_1131", "locus": "GCA000209735_01117" }, { "type": "cds", "sequence": "contig_1", "start": 619193, "stop": 619498, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAGTAAAAAGTTTATGAAGTTTATGGCAATTGGAACGATAGTTCCTTTAGCAAGTTTAACGACGAGTTGTTTTGGTCCTATTTTTGAGCCACATCAACCTCCAAATCCAAATCCGAACCCTAATCCAAACCCTAGTGGCGGAGAAAGCACGGATTGGAGCGATGTGGATGGCTATGTACATTATGATGCAGAAAAGAATCCAAAAGATTTTCTAAAGAAGGACAATCAATATTATTTTGCGCAAGTATTGCATAATGAGCAAAAATATGATGCCGATGTGTATAGAAATGATAATTGA", "aa": "MKKSKKFMKFMAIGTIVPLASLTTSCFGPIFEPHQPPNPNPNPNPNPSGGESTDWSDVDGYVHYDAEKNPKDFLKKDNQYYFAQVLHNEQKYDADVYRNDN", "aa_hexdigest": "5ae4a8cbd911d43c80787cf35874157b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11541.700899999993, "isoelectric_point": 5.83141918182373 }, "id": "OAIKOHHJFB_1132", "locus": "GCA000209735_01118" }, { "type": "cds", "sequence": "contig_1", "start": 619514, "stop": 619777, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAAAATCAATGTTCGTTTAGACTTGGATAAAGACTGGTATACTAGCCAACCTGAATTAAACGAAATTAAAGATTTATTCGATGTTGATTATAGCGCGCCGGTGAAGTTGAATAATATGATGAGAAACTTTTTGAATCCTGAATATAAAGATTTAGGTCTTGTGGATTCAAAGAATTATATTAATTCAATGGCTAGTTATTTAGAAGGATCGCAAAAAGATGTATTTGCCTATAAGGATAGTTATCATGGTAATAAATGA", "aa": "MPKINVRLDLDKDWYTSQPELNEIKDLFDVDYSAPVKLNNMMRNFLNPEYKDLGLVDSKNYINSMASYLEGSQKDVFAYKDSYHGNK", "aa_hexdigest": "ea19c6d5ae4af4eb3676eadbf877fc68", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10203.355799999998, "isoelectric_point": 4.955473518371581 }, "id": "OAIKOHHJFB_1133", "locus": "GCA000209735_01119" }, { "type": "cds", "sequence": "contig_1", "start": 619778, "stop": 620104, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGAGGACCAAAAGGAAGTAAGAAAACACTATCACTATTATTTTGATATGAAGCTTGATAATTACTATGAACAATTCATCAATGATTTTGCTCTTAAAGGCACAAGCAAGACATTTAAAGATATTGCTATTGATGGTTTATATAAACAAGGAATGTGGAATAATTATTTCGACTTAGGAAAAGATTATTCAAAATGGTTTTATTTAGCTTTTACAAGAAGTAATGTTGAAAAATACCATGAAGGCTTTAAGATTTATGAGACTGCAAAACAAAACAAAGATTTCATAATGCAATTGCCTACGGGTGATGATTATAATCTTTGA", "aa": "MFEDQKEVRKHYHYYFDMKLDNYYEQFINDFALKGTSKTFKDIAIDGLYKQGMWNNYFDLGKDYSKWFYLAFTRSNVEKYHEGFKIYETAKQNKDFIMQLPTGDDYNL", "aa_hexdigest": "46c6c097a53fd654c69c00bd8f34eab2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13261.713499999989, "isoelectric_point": 5.872456932067871 }, "id": "OAIKOHHJFB_1134", "locus": "GCA000209735_01120" }, { "type": "cds", "sequence": "contig_1", "start": 620129, "stop": 620494, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTGGTAAGATGGTTAAGCTAACGTTAGTTAATAACGAAGCTTATAAAAAGTATTTAGAGTTATTGCAAATGTTTGGAACTATTCAAGCTGGAAATAAGAAGTGGTTTGAAATTGAACAAGTATATAGAAATTATTTTAAAGAGTACTTGGACAAAGCAACAAATGCATTGCTTGATGTTCTTAAATACGGAGCAGTCTTTGGCGTGGAAATGGAAGAATTAGGCAAAGAGAAAAAAACCTTAGTTAAGATGTTTCCAGTAGCTATTGGGGATAATCAATTTGCTGATTATGAATATAGCTACGTATGGGCGTATAGAACACTATATGATGTGTTAATGCCTATGTATGGGGCTAATGATTGA", "aa": "MAGKMVKLTLVNNEAYKKYLELLQMFGTIQAGNKKWFEIEQVYRNYFKEYLDKATNALLDVLKYGAVFGVEMEELGKEKKTLVKMFPVAIGDNQFADYEYSYVWAYRTLYDVLMPMYGAND", "aa_hexdigest": "013ce869a4b1cb22bf3d6b450ba5ea08", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14177.287199999993, "isoelectric_point": 5.539209938049315 }, "id": "OAIKOHHJFB_1135", "locus": "GCA000209735_01121" }, { "type": "cds", "sequence": "contig_1", "start": 620498, "stop": 620704, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCATATAGTTTTACTGAATATGATAATCAAAAGTCAGGCAATACACCATATGCTCCAATTTACGAATACATAAACTTTTTTACAAATACTGTTTTTAAAGCTAAAGATAAAAGTTTTAAAGAACTTGAATTAAACACTAAATTTATGAATAAAATGCAAGAGGCTGAATATAAAAAAGAAGCTAAAAGTAAGTTTCATAGCTGA", "aa": "MPYSFTEYDNQKSGNTPYAPIYEYINFFTNTVFKAKDKSFKELELNTKFMNKMQEAEYKKEAKSKFHS", "aa_hexdigest": "b716a7d05e6a6399036cfb1c65aa8a41", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8168.116399999998, "isoelectric_point": 8.803818702697757 }, "id": "OAIKOHHJFB_1136", "locus": "GCA000209735_01122" }, { "type": "cds", "sequence": "contig_1", "start": 620876, "stop": 621067, "strand": "+", "frame": 2, "gene": null, "product": "Mbov_0395 family pilin-like conjugal transfer protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA4", "UniRef:UniRef90_C4XFA4" ], "nt": "ATGAAACCTATTGATACAGAAGGATTAAGTAATTTAACAGAGCAAGTACAGAAAGTTGGACATATCATATTTGGACTTGTGGCTGTGGTGGCTGTTATTAGCGTTATTATTACCTCGATTGTGGCGGGGATACAAAACTCACATGAAGATGATCCACAAAAAAGAAAGCAAAACAATAAAAAGATTTTGTGA", "aa": "MKPIDTEGLSNLTEQVQKVGHIIFGLVAVVAVISVIITSIVAGIQNSHEDDPQKRKQNNKKIL", "aa_hexdigest": "5087b9cbe03a20493b3e281279e3d943", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 620870, "stop": 621157, "observations": { "mutation": [ 621065, 621101 ], "stop": [ 621065, 621101 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFA4", "query_cov": 1.0, "subject_cov": 0.6631578947368421, "identity": 1.0, "score": 117.0, "evalue": 4.48e-33, "gene_start": 1, "gene_end": 63, "reference_start": 3, "reference_end": 65, "reference_sequence": "MKMKPIDTEGLSNLTEQVQKVGHIIFGLVAVVAVISVIITSIVAGIQNSHEDDPQKRKQNNKKILWAGIVLILVIVGWGIFELVISIQASGGKTS" }, "paralog": false, "identity": 0.968421052631579, "score": 172.0, "evalue": 2.78e-57, "description": "Internal stop codon at 621065, 621101. Nonsense mutation around 621065, 621101.", "psc": { "uniref90_id": "UniRef90_C4XFA4", "product": "Mbov_0395 family pilin-like conjugal transfer protein", "uniref50_id": "UniRef50_C4XFA4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFA4", "UniRef:UniRef50_C4XFA4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFA4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA4" ] } }, "id": "OAIKOHHJFB_1137", "locus": "GCA000209735_01123" }, { "type": "cds", "sequence": "contig_1", "start": 621157, "stop": 621405, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGGATTTTTTACCCGTGTGATGGAGATTTTCAAATATGGTCCAACATATGTATTGTATTTATTGCTATTTTTTGTATCGCTAATTGTTTTTATATTATTAGCGATTCTATTATCGGTTGTTAAACTAGCAACTTTCGATTTATTAAACTTTGTTATTTTTGGCATAATGCCGAGACAAAACATTTTGGACCATCAAAGCCCATATATGTATGTTAGATTTGCGATAGTTTCGGCGGTTATATGA", "aa": "MSGFFTRVMEIFKYGPTYVLYLLLFFVSLIVFILLAILLSVVKLATFDLLNFVIFGIMPRQNILDHQSPYMYVRFAIVSAVI", "aa_hexdigest": "594dbf9addb063641ab0662d22f90068", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9502.483199999995, "isoelectric_point": 9.222605323791505 }, "id": "OAIKOHHJFB_1138", "locus": "GCA000209735_01124" }, { "type": "cds", "sequence": "contig_1", "start": 621472, "stop": 621783, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAGTGCGTTGGTATTTAGTGTTAAAAGTTTGTTTATACTTTTAGCATTTCAAATTACTATTTTACTTTTTAATCTATTTGTACAAAAAATAATTACATTGATGATTGGCGAAGGGAATGATATTGTTGTTGCTTTTATAAATGGCTTGCATAGGTTGTTTTATCCTCCAAAATTAGCTGGGGATGTTAAAGATATTTTAATTCCTGCGACTACAAATCCTTTTGGCTTTACTATCAACTTGGATATTAGTTTTGGTAGTTTTGTGGCCTTAGCAAAGAAAACTGAACATTTAAACATTGGTAATTGA", "aa": "MRSALVFSVKSLFILLAFQITILLFNLFVQKIITLMIGEGNDIVVAFINGLHRLFYPPKLAGDVKDILIPATTNPFGFTINLDISFGSFVALAKKTEHLNIGN", "aa_hexdigest": "1f530bd3ff05364bbc363b2280c88bc1", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11381.43519999998, "isoelectric_point": 9.223765754699706 }, "id": "OAIKOHHJFB_1139", "locus": "GCA000209735_01125" }, { "type": "cds", "sequence": "contig_1", "start": 621636, "stop": 622076, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATATTTGCGTTAAAAATAGATTTACAGCACCGAAGAAAGCAAAAAGTATTTGTAGTCCTAGGAAAAATACCCCCACCGATAACATATTTCCCACATACTTATCCTTTCATATCCTTACTTTCTTGCCATCATCAAGCAAGCTCAAAGGTAAAATAATTGGAAATATTAAATACAAGAAGAATAACGGAAATATCTTTCCTATGGCATCCATCCCCCCTAAAACAAGTGGAATAGCAATAAACACCGCTCCAATAATTGATAGAAACGCTGCAGTTATTAAAATGGATGCTCAATTACCAATGTTTAAATGTTCAGTTTTCTTTGCTAAGGCCACAAAACTACCAAAACTAATATCCAAGTTGATAGTAAAGCCAAAAGGATTTGTAGTCGCAGGAATTAAAATATCTTTAACATCCCCAGCTAATTTTGGAGGATAA", "aa": "MNICVKNRFTAPKKAKSICSPRKNTPTDNIFPTYLSFHILTFLPSSSKLKGKIIGNIKYKKNNGNIFPMASIPPKTSGIAINTAPIIDRNAAVIKMDAQLPMFKCSVFFAKATKLPKLISKLIVKPKGFVVAGIKISLTSPANFGG", "aa_hexdigest": "be119545e9f8a00836c1c4d3d042829e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15852.893799999973, "isoelectric_point": 10.454209327697754 }, "id": "OAIKOHHJFB_1140", "locus": "GCA000209735_01126" }, { "type": "cds", "sequence": "contig_1", "start": 622603, "stop": 622806, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAATTTAAGGCGATGCCAAAGAAGTATTCTAATGTGGTGGATGACAAGAATGTAAGAGATAGTTCTGATTTAAATCCATATTCAACATTAGTAGAAAACGATGTTGTTAAAAACAAGTCATTTTCAAATAAGTTGTTTCAAAAGAGTGAACATAGTAATTACTTTAGTGTTTTTAAATTAGGTGGAAAAAATATATGA", "aa": "MFKFKAMPKKYSNVVDDKNVRDSSDLNPYSTLVENDVVKNKSFSNKLFQKSEHSNYFSVFKLGGKNI", "aa_hexdigest": "da513e353c81c917ec7655a69dc05219", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7754.720499999998, "isoelectric_point": 9.601872825622557 }, "id": "OAIKOHHJFB_1141", "locus": "GCA000209735_01127" }, { "type": "cds", "sequence": "contig_1", "start": 623047, "stop": 623394, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGAAACATTTTATTTAATTTTAAATGCTCCAACATTAGAAGAATTGGATGAGTGTTATTCGACATTTATTCAAAAGGCTGAAGATTATGAAGTTATTAGCTATGAAAAGCTAAGTGGCTTTGAGTTATTATCATTTTTGAATAAACTTAATCACTTTAATAAAAGCGAAGAAGATATTGAAACGTTTTTAAATGAAAGTGATATTTACGAAAGTGAATATAGTTTGGACCGCTTGTTCAAGTATGAAAAGGTGGAGTTTAAAAGCAATTCATTAAAAATTGATGATAAGTTGTATAGCGTTAAATGTGCTGGGAACATTCCAATACGCTTGAATAACGAGTGA", "aa": "MIETFYLILNAPTLEELDECYSTFIQKAEDYEVISYEKLSGFELLSFLNKLNHFNKSEEDIETFLNESDIYESEYSLDRLFKYEKVEFKSNSLKIDDKLYSVKCAGNIPIRLNNE", "aa_hexdigest": "7329907af2238aaf3f1e5143e18b2cfa", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13622.117199999982, "isoelectric_point": 4.459382820129394 }, "id": "OAIKOHHJFB_1142", "locus": "GCA000209735_01128" }, { "type": "cds", "sequence": "contig_1", "start": 623500, "stop": 623913, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACGCTGGTAATTTGGATAATTCAATTATTAATAGATTCGGTTCAGCATTAGAGGAAGAATCGATTGCGGTTATGATTGACCAAATACAAGCGGATAATTTTAAGTTATTTGATTCAAATATTTTTGTTTTAGCTGAAGCGGAAAATGCTGAAGAATTGAAGCAAAATAATTTGAAAATTAAGAATGGTTTTACAAGATCTAATTTTGGAATTAATGATTTGACCTTTAGACAATTTGAAGGATTTTTAGATATGTCTAATTATCCATTAAGCAAATTAGATAGAGAGTACTATCAAATCACAAGTATAAATAACGCAATAGGCTATCCATTGAAAAATCTGCCACTTAATGACGGAAACAACTTGTTGCTTGGAAATGAAGTGGGAGGAAATGATAGTCCTATTGTATGA", "aa": "MDAGNLDNSIINRFGSALEEESIAVMIDQIQADNFKLFDSNIFVLAEAENAEELKQNNLKIKNGFTRSNFGINDLTFRQFEGFLDMSNYPLSKLDREYYQITSINNAIGYPLKNLPLNDGNNLLLGNEVGGNDSPIV", "aa_hexdigest": "60571d7151425553be189582d3abe5bb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15326.890099999975, "isoelectric_point": 4.264652442932129 }, "id": "OAIKOHHJFB_1143", "locus": "GCA000209735_01129" }, { "type": "cds", "sequence": "contig_1", "start": 623917, "stop": 624363, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12846.13", "PFAM:PF01935.23", "PFAM:PF10412.15" ], "nt": "ATGTTTAGCTTGACTTCAAGTAGAGCAAATCATAATGTGATGATATTGGGAACTTCAGGAATGGGCAAAAGTACTTTAACCAAGAAGATTCTTACTTCATCACTGGCTGCGGGGAATATAGCAATTATTATTGATCCGCAAGCTGAATATGTTAAATGGGCAAATAAAATGGGAGGCCAAATAGTTGATTTAGGTTCTGGAGATGGAACGGTTATTAATCCATTACAAGTCAAATCATTTATTGCGCGTGAAGAAGATGATGCCAATGTTAATTTCAATACTTTAATTAATAATCATTTGCTTTGGTTAGAAAAGTTCTTTAGTTTCATTTTTGATGATATGAATGAAATGAAGAAGAACATTTTGCAACTTGAAATTATTAAGTTATATCAAAGTTGGGGCATTTATAGATTAAAGGAAGTTAAAGAACTCAAAAACGATGGATGA", "aa": "MFSLTSSRANHNVMILGTSGMGKSTLTKKILTSSLAAGNIAIIIDPQAEYVKWANKMGGQIVDLGSGDGTVINPLQVKSFIAREEDDANVNFNTLINNHLLWLEKFFSFIFDDMNEMKKNILQLEIIKLYQSWGIYRLKEVKELKNDG", "aa_hexdigest": "7bdeadcb03d81e2d1d7547110e406fd8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12846.13", "name": "AAA_10", "length": 49, "aa_cov": 0.3310810810810811, "hmm_cov": 1.0, "evalue": 5.631852800372788e-08, "score": 28.243488311767578, "start": 9, "stop": 57 }, { "id": "PF01935.23", "name": "DUF87", "length": 108, "aa_cov": 0.722972972972973, "hmm_cov": 0.7777777777777778, "evalue": 1.9954882902224044e-10, "score": 37.21625900268555, "start": 12, "stop": 118 }, { "id": "PF10412.15", "name": "TrwB_AAD_bind", "length": 76, "aa_cov": 0.5135135135135135, "hmm_cov": 0.881578947368421, "evalue": 2.0698584022879457e-08, "score": 29.528656005859375, "start": 4, "stop": 79 } ], "seq_stats": { "molecular_weight": 16628.021899999992, "isoelectric_point": 6.732430839538575 }, "id": "OAIKOHHJFB_1144", "locus": "GCA000209735_01130" }, { "type": "cds", "sequence": "contig_1", "start": 624370, "stop": 625176, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2982C0" ], "nt": "ATGGATGATTTGATTAAGCAAATGAAAAAGTTTGTAGCTCCTAAAGACCATCACGATAAAGAAGGCTATACTAAAAAGGTTTTAGATATGGCTGAATTATTAGAGGGTAAATTTGGAAGATATGGAACTTTGGGTGCGTTATATAATGGTCACACCAATATCAATATTGAAAACGATTTGGTTGTTTTTAAAACAAGTAATTTAATCGATACTGAAGGTAGCGTAAATGCAAGAATTGGCATTATGGTTTTAATTAGCTTGGTGAATGGCTTTATCTTTGATAATGCTTTTAATAACAAGAAAAGAATTAACGAATATAAAGAAAAAAATAAGATTCGTTTAATTAGCAAGAAAGAAATACAAAAGTTAACACGTTATTGTTCATTTTGTATTGATGAAGAACACTTGTATATTAGTGAAAAGAATATAACTACTTTGAACTATATATCAGATACTACAAAGCTTGTGCGTAAGCTTGATTGCGGGACAATACATACCACGCAAAATCCTAGTGATTATGCTTCAAGCACGGCAGTTGAACAAATTGCTAAAAAGATTACGGGGAACTGTCAATATTCATTTTTTCTAGGGTTAGTAGGAGATGATATTGAGGCGGTTAAAAAGTTATACAAAACGGCTGAAGCTCCATTACTAGATTCTGAAGTTAAGTTTATTTCAGGAAGAAGAGTGGGGAAAGTTTTAGCAAGTATGAGTAATGCACTTAGATACAAAATTAATCTACATTACAATCCAAAGGAAAAGGAATTATTCTTTGAAAAGGGGGAAGATGAAAACATGAGAAATTAG", "aa": "MDDLIKQMKKFVAPKDHHDKEGYTKKVLDMAELLEGKFGRYGTLGALYNGHTNINIENDLVVFKTSNLIDTEGSVNARIGIMVLISLVNGFIFDNAFNNKKRINEYKEKNKIRLISKKEIQKLTRYCSFCIDEEHLYISEKNITTLNYISDTTKLVRKLDCGTIHTTQNPSDYASSTAVEQIAKKITGNCQYSFFLGLVGDDIEAVKKLYKTAEAPLLDSEVKFISGRRVGKVLASMSNALRYKINLHYNPKEKELFFEKGEDENMRN", "aa_hexdigest": "fd4e47e07cfb58f2a4c9ea07298295fc", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI001F2982C0", "query_cov": 0.9776119402985075, "subject_cov": 0.9225352112676056, "identity": 0.622, "score": 325.0, "evalue": 4.81e-109, "valid": false, "uniref50_id": "UniRef50_UPI001F2982C0" }, "pscc": { "uniref50_id": "UniRef50_UPI001F2982C0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2982C0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 30615.79740000003, "isoelectric_point": 8.78963565826416 }, "id": "OAIKOHHJFB_1145", "locus": "GCA000209735_01131" }, { "type": "cds", "sequence": "contig_1", "start": 625445, "stop": 625840, "strand": "+", "frame": 2, "gene": null, "product": "ICEF Integrative Conjugal Element-II", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA7", "UniRef:UniRef90_C4XFA7" ], "nt": "TTGACTCCATTTTATCGCAAGATTATAAATGAAATTAACAAGCAAAATATTGATTTGGTTTTGCTTGATTTAAAAGTCAATATGTTGTTAAATGCTCTTATAAGCGATAAAGGAATGTTAGATGAACCTAACAATAATTTGCTTAAAGAGCCAGAATATGCAAAGCGTTTGCGTGAAAGAATGAATGAATCGGCTTCTGCATGGTATAAGAAGGTAATTAAAAAGGTAAGAAATGAAGACAATTCATTAAGCAAGTTTGCAGAACAATTCGAAGACAATGAAATTGAAAAAGTGTTTGAAAAAGAAAATGCAAAACTTGAAGCTTCAGAATTAAATAATGCTAAAGAGGATGAAGATTTTGGTGATTTTGATTTAAGTGAGTTTTTTAAAAAATAA", "aa": "MTPFYRKIINEINKQNIDLVLLDLKVNMLLNALISDKGMLDEPNNNLLKEPEYAKRLRERMNESASAWYKKVIKKVRNEDNSLSKFAEQFEDNEIEKVFEKENAKLEASELNNAKEDEDFGDFDLSEFFKK", "aa_hexdigest": "1e201de3510ce021ade96f9646eff4b6", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 625157, "stop": 625840, "observations": { "mutation": [ 625166, 625436 ], "stop": [ 625166, 625436 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFA7", "query_cov": 1.0, "subject_cov": 0.5745614035087719, "identity": 0.992, "score": 242.0, "evalue": 2.31e-79, "gene_start": 1, "gene_end": 131, "reference_start": 98, "reference_end": 228, "reference_sequence": "MKTWEISQENKKGDKLYVKSSRASKNGSKCISTRLSEPDYVALYERWVKRIKQSGSTPYVELKDIIRNALIKQEKEEIAKDFKNDLFYALRKVSWASLTPFYRKIINEINKQNIDLVLLDLKVNMLLNALISDKGMLDEPNNNLLKEPEYAKRLRERMNESASAWYKKVIKKVRNEDNSLSKFAEQFEDNEIEKVFEKENAKLEASELNNAKEDEDFGDFDLSEFFKK" }, "paralog": false, "identity": 0.9780701754385965, "score": 429.0, "evalue": 1.4e-155, "description": "Internal stop codon at 625166, 625436. Nonsense mutation around 625166, 625436.", "psc": { "uniref90_id": "UniRef90_C4XFA7", "product": "ICEF Integrative Conjugal Element-II", "uniref50_id": "UniRef50_C4XFA7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFA7", "UniRef:UniRef50_C4XFA7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFA7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFA7" ], "product": "ICEF Integrative Conjugal Element-II" } }, "id": "OAIKOHHJFB_1146", "locus": "GCA000209735_01132" }, { "type": "cds", "sequence": "contig_1", "start": 625886, "stop": 626347, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCACGCGGAGGCACGGGCGAAGAATGAGGAAAAAATGAATAAGAAAAAGATGTTAAAATTAATTCCATTGAGTGTATTGAGTATTGCGATTCCGTCAGTACTTAATTTATCGTTAAATACTAATAAAAAACTTCTAGGTTATGGAAAAGATTTTAAAGCTCCTGAAGAAAAACATAAGATTGATACTAATAGTTTATTTTCCAATTTTACTAGTATGGAAGATGATGAAATTGCTAAAAATGAAAGACTACAAAATAGGTATCAAGTAAAATGGTCACAACTATGTGATAAAGGATTATTGTATGCACAGGACCAATGGGTTGGTTATCATAAAACTGGCCCAGTAAAAAATAAAATTACGTCAATAGTTTCTAGGCCAACTTTAGGCTCTTTTGAAGTGCGTTATGAAAAAGCCTATTACACAAATCCTAGTAATCATAATAGTTATGGCTGGTTTTGA", "aa": "MHAEARAKNEEKMNKKKMLKLIPLSVLSIAIPSVLNLSLNTNKKLLGYGKDFKAPEEKHKIDTNSLFSNFTSMEDDEIAKNERLQNRYQVKWSQLCDKGLLYAQDQWVGYHKTGPVKNKITSIVSRPTLGSFEVRYEKAYYTNPSNHNSYGWF", "aa_hexdigest": "547e92dcc2bb3430ecf9e1292e14eeb4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17690.019999999993, "isoelectric_point": 9.477900123596193 }, "id": "OAIKOHHJFB_1147", "locus": "GCA000209735_01133" }, { "type": "cds", "sequence": "contig_1", "start": 626459, "stop": 626710, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGAACATAAAGGTGCTAGTTTTACTAACCTGTTTGCTGAATATAAAGTTTCTCAAAAAACATTAGAGTTTTTAAATAATCTTGAAACTGATAAAGATAAAAGGGTGTATAGTGGGCTAACTGATGATAAAAATAAAATGTTTTTGTGGGGGCTTCATGATTTTGATTGTGAAATTGAAGGAATGTATGATGTAAGGTACTATAATAATAAAGTATTAAATAATCTAGAATATGCTAAAGTTGGCTGA", "aa": "MEEHKGASFTNLFAEYKVSQKTLEFLNNLETDKDKRVYSGLTDDKNKMFLWGLHDFDCEIEGMYDVRYYNNKVLNNLEYAKVG", "aa_hexdigest": "9bda0e8721a59f6b7453f6ce3f3a7db8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9826.907499999998, "isoelectric_point": 5.06073932647705 }, "id": "OAIKOHHJFB_1148", "locus": "GCA000209735_01134" }, { "type": "cds", "sequence": "contig_1", "start": 626753, "stop": 627103, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAGATTTGAATATGGGGTAAATAAGAAAATTGACCAAAATCACTACTCTATAGATTTATTAAGCGATACCTTGGGCGATTTGACTACATCAGTTGATGGAGTTTTTAAAGACAATGGAGGCAAATCGAATATAGAACATTTAAACAAAATATTCGATAAAGAATATGAGTTTGAGTATGCTAGTGGAATTAAAGTTAAATACAAATATGATTACAAGATAAAAAACGAAGCTGTTGGTGAAAAGAACAAAATTGATGTTTATATTAAGGTTTTATCAGTCAAGATTGATGATAAGGAAATTATCGATAAAGACTTGGATAAGATTCTTAATAATATTGAAGACTGA", "aa": "MNRFEYGVNKKIDQNHYSIDLLSDTLGDLTTSVDGVFKDNGGKSNIEHLNKIFDKEYEFEYASGIKVKYKYDYKIKNEAVGEKNKIDVYIKVLSVKIDDKEIIDKDLDKILNNIED", "aa_hexdigest": "12bff277ab04de805d151ec37261b111", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13474.997999999983, "isoelectric_point": 5.077279472351074 }, "id": "OAIKOHHJFB_1149", "locus": "GCA000209735_01135" }, { "type": "cds", "sequence": "contig_1", "start": 627230, "stop": 627430, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATAAAAGACTTAGTGGAGCTTCGTTAGCTTCACGGTTAACTATATCAACTTCTATTACTAATCCTGATGGAAGTATAAGTCAAATAGAAGCAAAGACAAAAGCTAATGGTGGCGATTTAAAAAATCTAATTAAAAGAGACGAACCTGAAAGGGTAAGCAATAAAGAACTTGAAAAAGATTTTGTCGGTAAGCCATGA", "aa": "MYKRLSGASLASRLTISTSITNPDGSISQIEAKTKANGGDLKNLIKRDEPERVSNKELEKDFVGKP", "aa_hexdigest": "e3d98ceff45638283a1d2f30053658e5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7205.081999999999, "isoelectric_point": 9.453788948059085 }, "id": "OAIKOHHJFB_1150", "locus": "GCA000209735_01136" }, { "type": "cds", "sequence": "contig_1", "start": 627506, "stop": 627781, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAAATAAGCAAAAGGTTAGTGTTTTAAATAGAATATTTGAAAAGAAATTAATTGATGAAAGAGTTAATGTTAATGATGCATCTAAAATACCTTTTGATAAAAACTCGGGAAGCGGTAATGGCGAAGGCGATGTTGCAAAAAATGAATATCTTGTTGAAGTTTATAGATACAAGAAAGGCTCAAATAATAGTGGAGAGCCGGAAGAAAAGTATGTAGTTATTTATGAAGTAATTGGAAATCAGTTTAAACAAAACATAAAATATTATGCTTGA", "aa": "MVNKQKVSVLNRIFEKKLIDERVNVNDASKIPFDKNSGSGNGEGDVAKNEYLVEVYRYKKGSNNSGEPEEKYVVIYEVIGNQFKQNIKYYA", "aa_hexdigest": "57d3445016ece3a9d5ba8fc5ce158842", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10439.608, "isoelectric_point": 8.752115058898926 }, "id": "OAIKOHHJFB_1151", "locus": "GCA000209735_01137" }, { "type": "cds", "sequence": "contig_1", "start": 627797, "stop": 627940, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCAAAAACAAATTATTTCTCCATATATGCTTGATTCAAAGGGAAAAGAGTTAAAGGATAAAGAAGGCAAATTAATAGTAAATAAGAATTATGATCCAAGTATTGATAAAGAAACAGGAACTAAAAAGCAGATTGTTTGA", "aa": "MDQKQIISPYMLDSKGKELKDKEGKLIVNKNYDPSIDKETGTKKQIV", "aa_hexdigest": "819168f07f3459a3efeb395e0df65970", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5396.195300000002, "isoelectric_point": 8.96376476287842 }, "id": "OAIKOHHJFB_1152", "locus": "GCA000209735_01138" }, { "type": "cds", "sequence": "contig_1", "start": 627953, "stop": 628417, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAGTGATGAATTAAAAGAACAACTTCGTTTTGTATATCCTAATATTAAAATAACTACAGATAAAGCAGATCTTGGAATCTATGCTGAAGCTAATGTGCTTGGTAAGGGGGCATTAAGAAACTTGGTGGTTGATAAAGTTTTAGAGTCATCTTCAAGATACTATAAGTGTTTGTTGTTTAGAAAAGAAAATGACAAATACGTTGAGATTGGTAGGAAAGAACAAAAACTAATTGAAATACCGGTAAATAATGGGACAAGTGAATATAGTTATATGTCTGAGGAAGGCATTTGGCTATTATTTACTGTAAATGAAAAATCAATATCAAATGTTAATTTGGTATTAATTGATGCAAAGAGCGAGCCAAAAAGTTTTTTCTTGGATTCAATTGAAAACAAGATAGAAAAAATTAATAATCAAAGTGAAGATTATTTTGAAGCATTTTTAGATGGTGGCAATTGA", "aa": "MFSDELKEQLRFVYPNIKITTDKADLGIYAEANVLGKGALRNLVVDKVLESSSRYYKCLLFRKENDKYVEIGRKEQKLIEIPVNNGTSEYSYMSEEGIWLLFTVNEKSISNVNLVLIDAKSEPKSFFLDSIENKIEKINNQSEDYFEAFLDGGN", "aa_hexdigest": "14f3e53a5efaf9d6cffd2a93f9497e12", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17724.8618, "isoelectric_point": 4.860722923278809 }, "id": "OAIKOHHJFB_1153", "locus": "GCA000209735_01139" }, { "type": "cds", "sequence": "contig_1", "start": 628622, "stop": 628789, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTATGGAGATGAAACTAATGAAGCTATTAAAGAAAATTATAGAAGACAAGTTAAAGAAATCGTGCTTAATTTTGTTAAAAGAAATATTGCTAATAGTGCTAAATTAAAGGCAATTGGAATAACTGAAAATGAAATTAATGGTGGCTGGGTAATAGCTAATTGA", "aa": "MNYGDETNEAIKENYRRQVKEIVLNFVKRNIANSAKLKAIGITENEINGGWVIAN", "aa_hexdigest": "81fb4d5b440c05c797eb70b5ddfcd8b0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6251.0062, "isoelectric_point": 8.06539783477783 }, "id": "OAIKOHHJFB_1154", "locus": "GCA000209735_01140" }, { "type": "cds", "sequence": "contig_1", "start": 628892, "stop": 629584, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGTGGAACGGGTAAATACATAAATCACAGCATAACATTTAATTTGAAAAATAGTGCGTACCACGTGTACTATCCTCCTATTGATTTATCATCACTAGACATTCAAAAACACTTCGAAATTGAAATGGGAAAGAATATTAGTAATGATTCAAAAGAATTAAAAGAAAAGATTAGAAATAAGTTGATAGGGCTAATAGAAGAACAATTCAACTTATACAAAAACAAAACTGGCATAGAAATGTTATTGAATAAAGATATTAAAGTAGAAGGACTAGATAACGTTTTAGATTTACTAGTACAGGGGAATAAGTTTAAAGATGGTATTGATATTAAGATAAAAGGTATTAATGCTAATTTAATTTCAAGCACTAAGATTCATATTATTAATGATACTGAAGCACCAGCATTTGATTTATCACTACTTAATGATACACTAGTAAAAAATCCTATTGTTATATCGCAAAAAGAACCTGAGAAAATTACCAAAGATATTATAAATAAGTTAAATGAGATAACGATTGCTTCAGGTATCGAGTTTAAAAAAGATTATTTAGTTTCACTTGTAAATGCTTCATTCTATTTACTTGGTTTAGTAGCTGATATTGATAAATTAAATGAAGCTAAATATGAAAAATTCAAAAAAGTTTATGAAGAAACTAAAGCAAAGGTAATGGCTGAATATAATACTTGA", "aa": "MRGTGKYINHSITFNLKNSAYHVYYPPIDLSSLDIQKHFEIEMGKNISNDSKELKEKIRNKLIGLIEEQFNLYKNKTGIEMLLNKDIKVEGLDNVLDLLVQGNKFKDGIDIKIKGINANLISSTKIHIINDTEAPAFDLSLLNDTLVKNPIVISQKEPEKITKDIINKLNEITIASGIEFKKDYLVSLVNASFYLLGLVADIDKLNEAKYEKFKKVYEETKAKVMAEYNT", "aa_hexdigest": "765f71697b058a00437b3fa17058fe4a", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26199.983000000033, "isoelectric_point": 6.923068809509277 }, "id": "OAIKOHHJFB_1155", "locus": "GCA000209735_01141" }, { "type": "cds", "sequence": "contig_1", "start": 631017, "stop": 631466, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8W0", "UniRef:UniRef90_C4XFA9" ], "nt": "TTGCTTGAAAGTAATATAGATAAAGCAATTTATCAAGATACTTCTGTATTAAATAAAAATGATAAATCATTGATAGTCGATGACCTAAGTCGCCGTATAAATGCAATTCCTCCCGATTTAAATATAATAAAGATATCAAATAATTTAAAGCACAATTTAGTTTTATATACTAACGATGAGTTAATAAATAATCAGCTTTTATCTACATTGTCTAATAATAGTAATAAAGTTAAGCGTGCAACAAAAAACTATTTTGCTAATAATTTTTCAAAATCCCAATTCCTAATTAACACAAACACTTCTAATACAAAATATACAAAAGAAGCTTTAAAGAATATTAAATTATGTATTGGTAAAGAGAAAAATATAATTAATCAAAATGATTTTGAGTATGAATTAGATAAAGAAACTAGTAATTATTTTATTAGAGAAATTGCTCATAATGATTGA", "aa": "MLESNIDKAIYQDTSVLNKNDKSLIVDDLSRRINAIPPDLNIIKISNNLKHNLVLYTNDELINNQLLSTLSNNSNKVKRATKNYFANNFSKSQFLINTNTSNTKYTKEALKNIKLCIGKEKNIINQNDFEYELDKETSNYFIREIAHND", "aa_hexdigest": "d11fea40fd11324cc7c0bad85465fa78", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 630726, "stop": 631667, "observations": { "mutation": [ 630756, 630960, 631464 ], "stop": [ 630756, 630960, 631464 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFA9", "query_cov": 1.0, "subject_cov": 0.476038338658147, "identity": 0.993, "score": 280.0, "evalue": 6.88e-93, "gene_start": 1, "gene_end": 149, "reference_start": 98, "reference_end": 246, "reference_sequence": "MYNFYMSLELWRPLKITNPLLEDIFHLNIDTKFKIHPLIIFENTKNKTYYCIRLQTARKSSSKYNLIIDNSSYQTNEYWKNHESVVVTKDIFIIDKELLESNIDKAIYQDTSVLNKNDKSLIVDDLSRRINAIPPDLNIIKISNNLKHNLVLYTNDELINNQLLSTLSNNSNKVKRATKNYFANNFSKSQFLINTNTSNTKYTKEALKNIKLCIGKEKNIINQNDFEYELDKETSNYFIREIAHNDWKDLDKTKDNVNIYSFENIGFLSKSSKSESSYIDPYDNKNIEKAKDKVAKEIPIKESKTDKDKTKLK" }, "paralog": false, "identity": 0.987220447284345, "score": 610.0, "evalue": 1.65e-225, "description": "Internal stop codon at 630756, 630960, 631464. Nonsense mutation around 630756, 630960, 631464.", "psc": { "uniref90_id": "UniRef90_C4XFA9", "uniref50_id": "UniRef50_A0A454C8W0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFA9", "UniRef:UniRef50_A0A454C8W0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C8W0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8W0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17230.18999999998, "isoelectric_point": 8.557549476623539 }, "id": "OAIKOHHJFB_1156", "locus": "GCA000209735_01142" }, { "type": "cds", "sequence": "contig_1", "start": 631883, "stop": 632629, "strand": "+", "frame": 2, "gene": null, "product": "Mbov_0401 family ICE element transposase-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFB0", "UniRef:UniRef90_C4XFB0" ], "nt": "ATGTATAAAGATAAAACTACAGGCAAAAGACTAGTTATTTATCCGAGTGAAGGATTAGCTGATAACGGTAGAAATAAATATTATAAAGAGTGCGTCGAAAAATGTGTTGAAAACAATATATACGCTTCAAAAGGTGATAATAGTAAGCTACTTATTCCTTATCACGTTTATTATTACCATAGGGAACAATTAAAGAAAGAAGTATTAAATAATTTGACTTTAAAAGACTATAAAAATCTAGAGTACAAGACTATACAAATAGATATTGATGATGCTTATATTAGAGTGTCTTTTAAAGGTAGAAAAATAAAGGTTAGAGTGCGTATGGCGACTATACATACTTTGCTTAGCGGAAGTAGTGTAAAAGCCATAGAAAATAAGACCTCTATTATGCAGTTTTTACCACTTGCAAGTGAGTTTAAAAACTTCAATAGTGAAGAAGATTTTGAAAACAAAATTAGAGAAAGTTTAAACAGTTTATATAAAGAAAATTGTGAAATTATTGTCTCTGGAGATGGTGCAAAATTCATAACTACAATAGCTGATAATTTAAAATCAACGAGAGTTTATGATCCATTTCATTTCAAAGCAAATATGTTTAAAGCTTTTGGATATAGCAAAAAAATTAATGTAAGTAATAAGAGATTTTTTAGGGATTATAAAGATGTTTATCGCGTGTTAGACAACTTGTTTTCTAAAGGATTAATTTATGAATTTGAGAAAGAACTTGACAGTGTTTTAGAGTGA", "aa": "MYKDKTTGKRLVIYPSEGLADNGRNKYYKECVEKCVENNIYASKGDNSKLLIPYHVYYYHREQLKKEVLNNLTLKDYKNLEYKTIQIDIDDAYIRVSFKGRKIKVRVRMATIHTLLSGSSVKAIENKTSIMQFLPLASEFKNFNSEEDFENKIRESLNSLYKENCEIIVSGDGAKFITTIADNLKSTRVYDPFHFKANMFKAFGYSKKINVSNKRFFRDYKDVYRVLDNLFSKGLIYEFEKELDSVLE", "aa_hexdigest": "d325320591f32ac6814f3d3679059197", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 631709, "stop": 632881, "observations": { "mutation": [ 631763, 632627, 632714 ], "stop": [ 631763, 632627, 632714 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFB0", "query_cov": 1.0, "subject_cov": 0.6358974358974359, "identity": 1.0, "score": 491.0, "evalue": 3.51e-173, "gene_start": 1, "gene_end": 248, "reference_start": 59, "reference_end": 306, "reference_sequence": "MIMEGAVKNKMSKTLADNWNAQAEVFKNSEERKIKYNVERKIERDIFIWGETVRIIYYMYKDKTTGKRLVIYPSEGLADNGRNKYYKECVEKCVENNIYASKGDNSKLLIPYHVYYYHREQLKKEVLNNLTLKDYKNLEYKTIQIDIDDAYIRVSFKGRKIKVRVRMATIHTLLSGSSVKAIENKTSIMQFLPLASEFKNFNSEEDFENKIRESLNSLYKENCEIIVSGDGAKFITTIADNLKSTRVYDPFHFKANMFKAFGYSKKINVSNKRFFRDYKDVYRVLDNLFSKGLIYEFEKELDSVLEWITKQEKNKTVYANAKKFKKYYKENREYIWNSVLVKNYFGGCAETVVGHDLKRFCSKKFATFSLKTIQLSIAKNMETKANLFFI" }, "paralog": false, "identity": 0.9897435897435898, "score": 768.0, "evalue": 5.1e-285, "description": "Internal stop codon at 631763, 632627, 632714. Nonsense mutation around 631763, 632627, 632714.", "psc": { "uniref90_id": "UniRef90_C4XFB0", "product": "Mbov_0401 family ICE element transposase-like protein", "uniref50_id": "UniRef50_C4XFB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFB0", "UniRef:UniRef50_C4XFB0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFB0" ], "product": "ISLre2 family transposase" } }, "id": "OAIKOHHJFB_1157", "locus": "GCA000209735_01143" }, { "type": "cds", "sequence": "contig_1", "start": 633446, "stop": 634003, "strand": "+", "frame": 2, "gene": null, "product": "IS3 family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZVD0", "UniRef:UniRef90_UPI00003A869D" ], "nt": "TTGAAAAAGTCAACTTTTTATGAGATATTAAAATCACAAAATAAACCTGATAAAGATGAAAATTTAAAAAAGGTTATTTTTGACTTATTTAACTATAATAAAGGACTATACGGTTATAGACGTATTACTTTTGCTTTAAGAAATAAAGGAATAATAATCAATCATAAAAAAGTTCAAAAATTAATGAAAGCAATGAATATTTTCGGCAAAACGCTAAGAAGAAAAAATAAATATTCTTCATTCAAAGGTGATGCTCACAAAAACATTCCAAACTTGCTTTTAGATAAAGAAAATATCACAGAAGATTTCTTCAGATACAAAAGAAATTTTTCAAATAATAAATATTTGAAAATACTAGGAACAGATGTTACTGAATTTAAATTAAAAAATGATGAAAAAGCATATTTTTCTCCTGTAGTTGATTTTGAAAACAGAGAGATTTTAGGTTATTCGATTTCTAAATCGCCTAATTTAAGAATGGTTGGTAAAATGTTAGAAAACGTAGAAGAGAATGGCCACAGCTTAAAAAATGTATTATTACATTCTGATCAAGGATGA", "aa": "MKKSTFYEILKSQNKPDKDENLKKVIFDLFNYNKGLYGYRRITFALRNKGIIINHKKVQKLMKAMNIFGKTLRRKNKYSSFKGDAHKNIPNLLLDKENITEDFFRYKRNFSNNKYLKILGTDVTEFKLKNDEKAYFSPVVDFENREILGYSISKSPNLRMVGKMLENVEENGHSLKNVLLHSDQG", "aa_hexdigest": "e9ee43c1414342ade693b8ebf6d8de59", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00003A869D", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.935, "score": 347.0, "evalue": 1.23e-120, "valid": true, "product": "IS3 family transposase", "uniref50_id": "UniRef50_A0A448ZVD0" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZVD0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZVD0" ], "product": "Transposase, IS861" }, "genes": [], "id": "OAIKOHHJFB_1158", "locus": "GCA000209735_01144" }, { "type": "cds", "sequence": "contig_1", "start": 634562, "stop": 634942, "strand": "+", "frame": 2, "gene": null, "product": "IS256 family transposase", "db_xrefs": [ "GO:0004803", "SO:0001217", "UniRef:UniRef50_C4XFB2", "UniRef:UniRef90_C4XFB2" ], "nt": "ATGGCTATGAAAGAAGTATTTCAAAATACAATCCAAAAAATGATGAACAAAGAATTTGATAATTTTATAGGTTACGAAAAAATGATAATAAAGTTCAAAAAAAATTATAGAAATGATTTTTTTAAGAAAAATGTTAATAGTCAATATGGCCAAATGGAAATTGACATTCCAAGAGATCGTGAAGCAAAAATTGAACCTATAATCATCAAGAAGTGTGAACGTGATATTTCTGAATTAGTTGATATGATATTTGCTTTATATTCTAGAGGAATGTCAACAAGAGATGTAAATGATTTTATGTTTAGCAAATATGGTGTTAATTATTCTCCAGCACAAATAAGTCAATTAACTAACGAAATTGTCGAAGACGCAAGATTATGA", "aa": "MAMKEVFQNTIQKMMNKEFDNFIGYEKMIIKFKKNYRNDFFKKNVNSQYGQMEIDIPRDREAKIEPIIIKKCERDISELVDMIFALYSRGMSTRDVNDFMFSKYGVNYSPAQISQLTNEIVEDARL", "aa_hexdigest": "f68bd9483c5e921774c802e29186cde5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 634562, "stop": 635245, "observations": { "mutation": [ 634940, 635120 ], "stop": [ 634940, 635120 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFB2", "query_cov": 1.0, "subject_cov": 0.49606299212598426, "identity": 1.0, "score": 249.0, "evalue": 5.66e-82, "gene_start": 1, "gene_end": 126, "reference_start": 1, "reference_end": 126, "reference_sequence": "MAMKEVFQNTIQKMMNKEFDNFIGYEKMIIKFKKNYRNDFFKKNVNSQYGQMEIDIPRDREAKIEPIIIKKCERDISELVDMIFALYSRGMSTRDVNDFMFSKYGVNYSPAQISQLTNEIVEDARLWQERKLETYYSIIYIDAVHFHVVDNNVVTKKATYVIMGINSDGQKEILGLYIGENESTKFWMSVLNALKNRGISKIDIICSDNLKGIQQSIEVVFPNSKQQRCIVHMIRNSVKWYERILQRFKICISI" }, "paralog": false, "identity": 0.9911894273127754, "score": 443.0, "evalue": 6.91e-161, "description": "Internal stop codon at 634940, 635120. Nonsense mutation around 634940, 635120.", "psc": { "uniref90_id": "UniRef90_C4XFB2", "product": "IS256 family transposase", "uniref50_id": "UniRef50_C4XFB2", "go_ids": [ "GO:0004803" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFB2", "UniRef:UniRef50_C4XFB2", "GO:0004803" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFB2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFB2" ], "product": "Mutator family transposase" } }, "id": "OAIKOHHJFB_1159", "locus": "GCA000209735_01145" }, { "type": "cds", "sequence": "contig_1", "start": 635123, "stop": 635281, "strand": "+", "frame": 2, "gene": null, "product": "transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0028527D83", "UniRef:UniRef90_UPI0028527D83" ], "nt": "ATGTCTGTCCTAAATGCTCTTAAAAATAGAGGAATTAGTAAAATTGATATTATTTGCTCAGATAATTTAAAAGGTATACAACAATCTATAGAAGTTGTTTTTCCTAATAGTAAGCAACAACGTTGCATTGTACATATGATTAGAAATTCTGTTAAATGA", "aa": "MSVLNALKNRGISKIDIICSDNLKGIQQSIEVVFPNSKQQRCIVHMIRNSVK", "aa_hexdigest": "4d2caef1bccf1cce25b0674586a48bbd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 634973, "stop": 635266, "observations": { "mutation": [ 635120 ], "stop": [ 635120 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0028527D83", "query_cov": 0.9038461538461539, "subject_cov": 0.47474747474747475, "identity": 0.915, "score": 89.4, "evalue": 4.19e-22, "gene_start": 1, "gene_end": 47, "reference_start": 51, "reference_end": 97, "reference_sequence": "MIYIDAVHFHVVDNNVVTKKATYVIMGINGEGQKEILGLYIGENESAKFWMSVLNALKNRGISKINIICSDNLKGIQQAIEAVFPDSKQQRCIVHMIEI" }, "paralog": false, "identity": 0.9072164948453608, "score": 177.0, "evalue": 1.67e-59, "description": "Internal stop codon at 635120. Nonsense mutation around 635120.", "psc": { "uniref90_id": "UniRef90_UPI0028527D83", "product": "transposase", "uniref50_id": "UniRef50_UPI0028527D83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0028527D83", "UniRef:UniRef50_UPI0028527D83" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0028527D83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0028527D83" ], "product": "transposase" } }, "id": "OAIKOHHJFB_1160", "locus": "GCA000209735_01146" }, { "type": "cds", "sequence": "contig_1", "start": 635283, "stop": 635375, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAATTTTGCAAAGATTTAAAATCTGTATATCAATCTAATGATGCAAAAAATGCAATGGATAATTTGGATATTTTTGAAGATAAATGA", "aa": "MKEFCKDLKSVYQSNDAKNAMDNLDIFEDK", "aa_hexdigest": "41d0c69f95dae21c1992124a4f6617f6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3540.9487, "isoelectric_point": 4.6018781661987305 }, "id": "OAIKOHHJFB_1161", "locus": "GCA000209735_01147" }, { "type": "cds", "sequence": "contig_1", "start": 635436, "stop": 635603, "strand": "+", "frame": 3, "gene": null, "product": "Mutator family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1I1BC38" ], "nt": "ATGTTTGATTACTCCCTTGAAATAAGAACGCTCATTTATACCACTAATCCAATCGAAAATTTAAATAGTGTATTTAGAAAATATGCAAAAACAAAAAAGGTTTTCCCATCTGATGATAGTCTTTTAAAAATTCTTTTTCTAGCATCTCAACAAATCATAAAAAAATGA", "aa": "MFDYSLEIRTLIYTTNPIENLNSVFRKYAKTKKVFPSDDSLLKILFLASQQIIKK", "aa_hexdigest": "d72a3c85770b93ad34e400e9ef47f997", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1I1BC38", "query_cov": 0.9818181818181818, "subject_cov": 0.8852459016393442, "identity": 0.574, "score": 65.9, "evalue": 3.47e-13, "valid": false, "product": "Mutator family transposase", "uniref50_id": "UniRef50_A0A1I1BC38" }, "pscc": { "uniref50_id": "UniRef50_A0A1I1BC38", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1I1BC38" ], "product": "Mutator family transposase" }, "genes": [], "id": "OAIKOHHJFB_1162", "locus": "GCA000209735_01148" }, { "type": "cds", "sequence": "contig_1", "start": 635854, "stop": 635985, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAGCACAAAAAAAATTGTAGCTTTAACAATGGGTATTTCTGCTGGGGTTGTTGGCGCAGTATCAGCCGGTACTTTAATTGGTTATGCAGTTGACCATTCAAACTATAAAAAAGAAAAATTGTGA", "aa": "MKNSTKKIVALTMGISAGVVGAVSAGTLIGYAVDHSNYKKEKL", "aa_hexdigest": "642402a76e340460a2aa707beead737e", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4452.200899999999, "isoelectric_point": 9.778580665588379 }, "id": "OAIKOHHJFB_1163", "locus": "GCA000209735_01149" }, { "type": "cds", "sequence": "contig_1", "start": 636115, "stop": 636579, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7", "UniRef:UniRef90_C4XFE7" ], "nt": "TTGTGCCAAACTCAAATTGATTCAAATGAAAAACTTATTAAAGATTTACAAGGAATTATTGATCAACACTCTTCATATCATGTTGGCCGCCACTTTGGTAACAAAAGTTATATAAGAGCTGCTAAACACAGTAATGAAAAGAAAATAATTCAAAATCTTTTACCAGAGATGATAGAAAGAACAAAACAAGTTATTAATCAAAGCGAATATGAAAATATCAAAAAATCAATCCAAGATTTTATTGTTCGTAGTAATGATTTATTAACTTTAAAAACCGCAGATGAAAAATTTGTTGATGCTAATAGTAAAAGTGAATTAGATTCTTTAGAACTCAAAATTAAAGATTATCAAAACATTTTAAGTAAAATCGAAGAAGAAGCACTTTCAAACAACACAAAACCTAACATTTCAACTGAAATGATTGATAAGGCAGCAGATTTTATTAAATCTGATTTTCATGTTTGA", "aa": "MCQTQIDSNEKLIKDLQGIIDQHSSYHVGRHFGNKSYIRAAKHSNEKKIIQNLLPEMIERTKQVINQSEYENIKKSIQDFIVRSNDLLTLKTADEKFVDANSKSELDSLELKIKDYQNILSKIEEEALSNNTKPNISTEMIDKAADFIKSDFHV", "aa_hexdigest": "d58bf7ad8ae3df16d904eca596400295", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 635854, "stop": 636615, "observations": { "mutation": [ 635983, 636061, 636085, 636100, 636577 ], "stop": [ 635983, 636061, 636085, 636100, 636577 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE7", "query_cov": 1.0, "subject_cov": 0.6039215686274509, "identity": 0.935, "score": 271.0, "evalue": 2.78e-90, "gene_start": 1, "gene_end": 154, "reference_start": 88, "reference_end": 241, "reference_sequence": "MKNSTKKIVALTMDISAGIVGAVSAGTLIGYAVDHSNYKKEKLWIENNIKYLKESNQKLKEKDNKLKEIWKKYENQEWLSSLWNELSLCQTQIDSNEKLIKDLQGIIDQHSSYHVGRRFGNKSYMRAVKHSNEKKIIQNLLPEMVERTKQVINQSEYENIKKSIQDFVVRSNDLLTLKTADEKFVDANSKSELNSLELKIKDYQNTLSKIEEEALSSNTKPNISTEMINKAADFIKSDFHVWNDKIIKKYVKKTA" }, "paralog": false, "identity": 0.8932806324110671, "score": 432.0, "evalue": 5.41e-156, "description": "Internal stop codon at 635983, 636061, 636085, 636100, 636577. Nonsense mutation around 635983, 636061, 636085, 636100, 636577.", "psc": { "uniref90_id": "UniRef90_C4XFE7", "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE7", "UniRef:UniRef50_C4XFE7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17768.86899999999, "isoelectric_point": 5.962433052062988 }, "id": "OAIKOHHJFB_1164", "locus": "GCA000209735_01150" }, { "type": "cds", "sequence": "contig_1", "start": 636634, "stop": 637266, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8", "UniRef:UniRef90_C4XFE8" ], "nt": "ATGTCAAACATATCTAAAATAGAAGAATTATCAGCAAAAATAGAAGATGCTAATACTATTGCAAGTAATAATTCTCCAATTTTAAATCCTATCAATGCTGTTAAAAAGATTTTATTGTCAAGATTTGACTCTAATAATTATGACGTTATATTAGAAAAAAACGGTGTTTCAGTAAATAGAAGTGTTATTATAGAAGATATTATTAAGGCATGTAAAACATTGGAACAATCATATGGAAATACAAAAGAATATATAAATCGTTATGTTAATACTTTTGTCAAAACAATTGAAAATACAAAATTATTTAACAAATCTCAAATTCTAGACTTAAGCAGCAAATCATTAACATCTATTATTAATGGTCTTAATGAATTTAATTCATTTGTTGAAAGTTCAAATCAAATTTCTTTTGAAAAATTAGACGAATTTATTAACAAAACTCAAGCTATTTTAAATGACGAAAATAATTTGACAACTTCTATTCAATCTGTTATTAAAGAATTTTTAAATGCTTATAATTCAAATGTTGATTCAATTGAAACAACAGTTAGATTAGCTAAACTAAATAACTCAAAACAAATTATTGATGAATTTGTACAAAAATCTAAAATTGAATTAAATTCTCTCAAATGA", "aa": "MSNISKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILEKNGVSVNRSVIIEDIIKACKTLEQSYGNTKEYINRYVNTFVKTIENTKLFNKSQILDLSSKSLTSIINGLNEFNSFVESSNQISFEKLDEFINKTQAILNDENNLTTSIQSVIKEFLNAYNSNVDSIETTVRLAKLNNSKQIIDEFVQKSKIELNSLK", "aa_hexdigest": "beb44c1c06e9e97a51f7aebf68fc9063", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 636634, "stop": 637464, "observations": { "mutation": [ 637264, 637345 ], "stop": [ 637264, 637345 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE8", "query_cov": 1.0, "subject_cov": 0.7608695652173914, "identity": 0.9570000000000001, "score": 369.0, "evalue": 2.45e-127, "gene_start": 1, "gene_end": 210, "reference_start": 1, "reference_end": 210, "reference_sequence": "MSNIDKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILEKNGVSVNRSVIIEDIIKACKTLEQSYGNTKEYINRYVNTFVKTIENTKLFNKSQILDLSNKSLTPIINGLNEFNSFVESSNQISFEKLDEFINKIQAILNVENDLTTSIQSVIKEFLNAYNSNADSIETTVKLAKLNNSKQIIDEFVQKSKIELSSLKWDNYIEGRNKLINEYVNSKNELDSIKNGFKKAFVNIKSRLNEFNLTLEANKTTLNEFYLFETSYKS" }, "paralog": false, "identity": 0.9420289855072463, "score": 494.0, "evalue": 3.08e-179, "description": "Internal stop codon at 637264, 637345. Nonsense mutation around 637264, 637345.", "psc": { "uniref90_id": "UniRef90_C4XFE8", "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE8", "UniRef:UniRef50_C4XFE8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 23710.505700000023, "isoelectric_point": 5.4200754165649405 }, "id": "OAIKOHHJFB_1165", "locus": "GCA000209735_01151" }, { "type": "cds", "sequence": "contig_1", "start": 637996, "stop": 638163, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGCAAAATTAGCAGCAATAGCAAATGTGGATTCTAAAGTATATGAATATAAAAACAAAATTTTTGATGCTGCAATTGAAACTTATAAAAATGTTAATTCTACAGAAAACTTTAGTGCTGTTCAAGCTGATAAAGACAACAAAGTTAACTTTGTTAAAAAATGA", "aa": "MDAKLAAIANVDSKVYEYKNKIFDAAIETYKNVNSTENFSAVQADKDNKVNFVKK", "aa_hexdigest": "1160043971d8aa939f463149be73f6fc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6202.908700000004, "isoelectric_point": 7.942263221740723 }, "id": "OAIKOHHJFB_1166", "locus": "GCA000209735_01152" }, { "type": "cds", "sequence": "contig_1", "start": 638275, "stop": 638493, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCAATTAAAAATGATCAAATTTCTAAATTAGATAGTGAAGGTTTAAAAGCTTTGCAACAACTTGATGGTGGCGTTGATTTTACAAACACTAATTTAATTAAAAATTATTTAGAAAATGATTATTTATTAGCTGTTAAAGATTTAAGAGAATATTTCATAAACAAACTAATTTACTTATTACATGAAAAATTCAATTATGGTGATGTATACTGA", "aa": "MNAIKNDQISKLDSEGLKALQQLDGGVDFTNTNLIKNYLENDYLLAVKDLREYFINKLIYLLHEKFNYGDVY", "aa_hexdigest": "15caef378a4fc0f00bb59b16fd4d993f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8418.452699999998, "isoelectric_point": 4.867032051086425 }, "id": "OAIKOHHJFB_1167", "locus": "GCA000209735_01153" }, { "type": "cds", "sequence": "contig_1", "start": 638992, "stop": 639156, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTCAAATATCAAAAAAATGTCAGATTCTAAAATAATGTTTAAAGATGATTTTCAAGGCAATTTTGTTAATTTAAATGATGCTCTTTACAAAATAAAATATGCTTTTGATTTTATTAAATACAACAATCCAACAAGCAATGGATATTATGAAGGAATATGA", "aa": "MLSNIKKMSDSKIMFKDDFQGNFVNLNDALYKIKYAFDFIKYNNPTSNGYYEGI", "aa_hexdigest": "18bfa3abdb1bd2c335e94e6ecba71249", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6356.1726, "isoelectric_point": 7.940844917297363 }, "id": "OAIKOHHJFB_1168", "locus": "GCA000209735_01154" }, { "type": "cds", "sequence": "contig_1", "start": 639297, "stop": 639422, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGCTGAATTATGTTCTACTGTTCTTAAATATGAGAATAATGTATTAGAAAATGGCATATATTCAAATAGTGGGTTAGTACTATCATATTTTTCATCTGATTTTCCACGTTTTGTTATATAG", "aa": "MKAELCSTVLKYENNVLENGIYSNSGLVLSYFSSDFPRFVI", "aa_hexdigest": "beb5d1d27752508245a5a3598068f700", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4649.2559, "isoelectric_point": 4.867770957946777 }, "id": "OAIKOHHJFB_1169", "locus": "GCA000209735_01155" }, { "type": "cds", "sequence": "contig_1", "start": 639956, "stop": 640129, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013354617.1", "SO:0001217", "UniParc:UPI0001E330E8", "UniRef:UniRef100_A0AB32XBI3", "UniRef:UniRef50_C4XFB5", "UniRef:UniRef90_C4XFB5" ], "nt": "TTGTTAAAATTAAGATATGAAAAATTTAAAGTCAAAGTTACTTCAAAAAAATATTTAAAATTTTTACCTGAAAATGAAACGTATAGATTCATAGTCGATTATATCGACAATAAAACAGAAATTTTTTTAAACAACTCTTCAATTGAATTAACTGAAAAAATGTTTGTTTCATAG", "aa": "MLKLRYEKFKVKVTSKKYLKFLPENETYRFIVDYIDNKTEIFLNNSSIELTEKMFVS", "aa_hexdigest": "3a10f14dffe7222b9b1a7448407b93a7", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E330E8", "ncbi_nrp_id": "WP_013354617.1", "uniref100_id": "UniRef100_A0AB32XBI3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330E8", "RefSeq:WP_013354617.1", "UniRef:UniRef100_A0AB32XBI3" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XBI3", "uniref90_id": "UniRef90_C4XFB5" }, "psc": { "uniref90_id": "UniRef90_C4XFB5", "uniref50_id": "UniRef50_C4XFB5" }, "pscc": { "uniref50_id": "UniRef50_C4XFB5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFB5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6974.100399999999, "isoelectric_point": 9.260125923156735 }, "id": "OAIKOHHJFB_1170", "locus": "GCA000209735_01156" }, { "type": "cds", "sequence": "contig_1", "start": 640256, "stop": 640504, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013526834.1", "SO:0001217", "UniParc:UPI0001F5A770", "UniRef:UniRef100_C4XFB6", "UniRef:UniRef50_C4XFB6", "UniRef:UniRef90_C4XFB6" ], "nt": "ATGCATGAAATAAGTAAAAACATCTTTGCTTACTATACTTCAAGCTTACAAAAAACTTATGAGTCAATTAACAAAGAAGAAATTAAAGAGTATTGCAATTTATTAAGCGAAACTAAAAATCACATATTTTTTGGTATTGGGCAAAGTGAAAAAGTAGCTAGTTATTTACGTGAAAATTTAAACAAAATTAGACTTACTTCATTACCTATTAACAATATGCATGATTTTTTTAACATAGTTTATGTTTAA", "aa": "MHEISKNIFAYYTSSLQKTYESINKEEIKEYCNLLSETKNHIFFGIGQSEKVASYLRENLNKIRLTSLPINNMHDFFNIVYV", "aa_hexdigest": "6cb27aec406fca34ebacc45794ec036a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F5A770", "ncbi_nrp_id": "WP_013526834.1", "uniref100_id": "UniRef100_C4XFB6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5A770", "RefSeq:WP_013526834.1", "UniRef:UniRef100_C4XFB6" ] }, "ips": { "uniref100_id": "UniRef100_C4XFB6", "uniref90_id": "UniRef90_C4XFB6" }, "psc": { "uniref90_id": "UniRef90_C4XFB6", "uniref50_id": "UniRef50_C4XFB6" }, "pscc": { "uniref50_id": "UniRef50_C4XFB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFB6" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 640136, "stop": 640504, "observations": { "mutation": [ 640223 ], "stop": [ 640223 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFB6", "query_cov": 1.0, "subject_cov": 0.6721311475409836, "identity": 1.0, "score": 166.0, "evalue": 9.8e-52, "gene_start": 1, "gene_end": 82, "reference_start": 41, "reference_end": 122, "reference_sequence": "MTRFARAASFISFKSLQIYITKRTIVTDDWTRINVGSNLSMHEISKNIFAYYTSSLQKTYESINKEEIKEYCNLLSETKNHIFFGIGQSEKVASYLRENLNKIRLTSLPINNMHDFFNIVYV" }, "paralog": false, "identity": 0.9836065573770492, "score": 238.0, "evalue": 2.16e-82, "description": "Internal stop codon at 640223. Nonsense mutation around 640223.", "psc": { "uniref90_id": "UniRef90_C4XFB6", "uniref50_id": "UniRef50_C4XFB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFB6", "UniRef:UniRef50_C4XFB6" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFB6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9712.957199999992, "isoelectric_point": 6.821440696716309 }, "id": "OAIKOHHJFB_1171", "locus": "GCA000209735_01157" }, { "type": "cds", "sequence": "contig_1", "start": 640504, "stop": 640611, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTCCAGATGCTTTTGTTACAGTTATTTCGCATTCTTTTGAAACAGCTGAAGTTATAAAAGTTTGTGAAATTTTGGAAAACAAGAACATTAATTATGCTTTATGA", "aa": "MGPDAFVTVISHSFETAEVIKVCEILENKNINYAL", "aa_hexdigest": "d3df982869dcd29e230ffbbbb843dc32", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3896.4423000000006, "isoelectric_point": 4.571128273010253 }, "id": "OAIKOHHJFB_1172", "locus": "GCA000209735_01158" }, { "type": "cds", "sequence": "contig_1", "start": 641085, "stop": 641711, "strand": "+", "frame": 3, "gene": "upp", "product": "uracil phosphoribosyltransferase", "db_xrefs": [ "EC:2.4.2.9", "GO:0004845", "GO:0009116", "RefSeq:WP_013354614.1", "SO:0001217", "UniParc:UPI0001A8C2FF", "UniRef:UniRef100_C4XFB8", "UniRef:UniRef50_Q98QP6", "UniRef:UniRef90_C4XFB8" ], "nt": "ATGTTAAAAGTTTTAGATCATCCGCTTATTTCAATTAAGCTGACTAATATGAGAGATAAAGAAGCAAATCACTCTCGTTTTAGAAGAAACTTAAATGAAATCGCATCATTAATGGTGTATGAAGTTTTAAGAAACTATAAAACTAAAGCTAGAAAAATAGTGACACCTTTAAATCAAGAATATGTAGGAGCTACTTTTGATAAAGAAATAGTTATTGTGCCAATTTTAAGAGCTGGGCTTGGAATGTTAGATGGCTTACTTGAATTAATGCCTGAAGCTAGAGTTGGTCACATTGGACTTTACCGCGATGAAGTAACTCATCAAGCTCATGAGTATTTTTACAAAATGCCAGAAGTTAAAAAAGATAGCTACATTTTTGTAGTTGATCCAATGCTCGCTACTGGCGGTAGCGCAGTTGATGCTATTAAAAAATTAAAATCAGATGGCTTTACTAATATTCATTTAGTGTGCTTAGTTGGAGTTAAAGAAGGAGTAGCAGCTGTTGAAAAGGCTTTTGGTAAAGACTTTGAAATTTATTTAGCTGCTCTAGACAAAAAACTCAATAATCAAAAATATATTGAACCTGGTTTAGGCGACGCAGGAGACCGTATTTTTGGAACAAAATAA", "aa": "MLKVLDHPLISIKLTNMRDKEANHSRFRRNLNEIASLMVYEVLRNYKTKARKIVTPLNQEYVGATFDKEIVIVPILRAGLGMLDGLLELMPEARVGHIGLYRDEVTHQAHEYFYKMPEVKKDSYIFVVDPMLATGGSAVDAIKKLKSDGFTNIHLVCLVGVKEGVAAVEKAFGKDFEIYLAALDKKLNNQKYIEPGLGDAGDRIFGTK", "aa_hexdigest": "257b399ec7c1330e5d86530cc756a37d", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001A8C2FF", "ncbi_nrp_id": "WP_013354614.1", "uniref100_id": "UniRef100_C4XFB8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C2FF", "RefSeq:WP_013354614.1", "UniRef:UniRef100_C4XFB8" ] }, "ips": { "uniref100_id": "UniRef100_C4XFB8", "uniref90_id": "UniRef90_C4XFB8" }, "psc": { "uniref90_id": "UniRef90_C4XFB8", "gene": "upp", "product": "uracil phosphoribosyltransferase", "ec_ids": [ "2.4.2.9" ], "uniref50_id": "UniRef50_Q98QP6", "go_ids": [ "GO:0004845", "GO:0009116" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QP6" ], "product": "Uracil phosphoribosyltransferase" }, "genes": [ "upp" ], "id": "OAIKOHHJFB_1173", "locus": "GCA000209735_01159" }, { "type": "cds", "sequence": "contig_1", "start": 641698, "stop": 641892, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAACAAAATAAAAGTATTTATTATGGCAAAAGTATTGTTAAAAAAATAGTCCAAACTGGAGTTTTATTAGCTTTAGCAATTGTAGTTAATATTACTTGCTCAAGCTTTATGAAATTTCCTTTAGCGCCTTTTTTAAAGTTTGATTTTGCACTTGTTGTCGTTACTTTTAGCGCTTTAGCAATTGCACCTTGA", "aa": "MEQNKSIYYGKSIVKKIVQTGVLLALAIVVNITCSSFMKFPLAPFLKFDFALVVVTFSALAIAP", "aa_hexdigest": "759bf4df9aeac6cfbb6557141f6737f0", "start_type": "TTG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6996.426699999998, "isoelectric_point": 9.581758689880367 }, "id": "OAIKOHHJFB_1174", "locus": "GCA000209735_01160" }, { "type": "cds", "sequence": "contig_1", "start": 642614, "stop": 642733, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGTTCCCTTATCCAAGTGGAAATATTCATATGGGGCATGTTAGAAACTATGCAATCGGCGATGTTTTAGCACGTTATTATCGTCGTAAAGGTTTTAATGTTTTACATCCTTTTGGTTGA", "aa": "MFPYPSGNIHMGHVRNYAIGDVLARYYRRKGFNVLHPFG", "aa_hexdigest": "d46ec1b438548f1f01d589ed7806e043", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 37, "aa_cov": 0.9487179487179487, "hmm_cov": 1.0, "evalue": 6.56202043513904e-08, "score": 27.757923126220703, "start": 3, "stop": 39 } ], "seq_stats": { "molecular_weight": 4552.2083, "isoelectric_point": 10.15604305267334 }, "id": "OAIKOHHJFB_1175", "locus": "GCA000209735_01161" }, { "type": "cds", "sequence": "contig_1", "start": 643190, "stop": 643651, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13603.12" ], "nt": "TTGGCAAAATTTGACATAGTTGAAAATAATCAAAATCACTTTAGTAATATTGATATTTTTACAAAAAACAAAGATATTTTAGTAACCATGAATTTTATAACTATTGGTGCAAATCATGGCCTTGTTAAACAATTAATTGAAGAAAATAAATTAAGTGATAAAGAAATTGAAAAACTTAATTTAATTAAAGCAAAAGCTACAGCAAAAGATTTTTCGCAAAAATTATGTTTAGCTTTACCACTTCAAGCAATTAATCCAGTAAATAATCAAAAATACAAAATTTATGTTAGTGATTTTTGTTCTCTCGGAGAAAAAAATAGAAGTCAAATTATCGATACTACTAAAGTTAAAAGTCATGAAGAATTTGCCAAATTTAATAACTTAGAAATTACTAATTATGCAAATAACAAAGCTGATTTTAGTAAGCTTGAAAACTCGAGTAAAATCAACTTACAAGATTGA", "aa": "MAKFDIVENNQNHFSNIDIFTKNKDILVTMNFITIGANHGLVKQLIEENKLSDKEIEKLNLIKAKATAKDFSQKLCLALPLQAINPVNNQKYKIYVSDFCSLGEKNRSQIIDTTKVKSHEEFAKFNNLEITNYANNKADFSKLENSSKINLQD", "aa_hexdigest": "3270587a8835bf9ee5d81e0e62c0dc66", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 128, "aa_cov": 0.8300653594771242, "hmm_cov": 0.9921875, "evalue": 1.7578792444818714e-13, "score": 46.50330352783203, "start": 13, "stop": 139 } ], "seq_stats": { "molecular_weight": 17468.729499999994, "isoelectric_point": 8.439249992370609 }, "id": "OAIKOHHJFB_1176", "locus": "GCA000209735_01162" }, { "type": "cds", "sequence": "contig_1", "start": 643691, "stop": 643810, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCAATTGTGCTAAGTGTGGTTTAGTTCCTGAAAAAGAAGAAAACTTACCAGTTACTTTGCCTTACAAAGTCAACTTTACTACTAGTGGCAACCCACTTAAAACAAATAATAAATGA", "aa": "MINCAKCGLVPEKEENLPVTLPYKVNFTTSGNPLKTNNK", "aa_hexdigest": "9efae394d5bc670e937c1677f55fc07d", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4306.978400000002, "isoelectric_point": 8.737867546081542 }, "id": "OAIKOHHJFB_1177", "locus": "GCA000209735_01163" }, { "type": "cds", "sequence": "contig_1", "start": 644009, "stop": 644245, "strand": "+", "frame": 2, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002381B5B9" ], "nt": "TTGCACTTACTGTATGCACGTTTCTTTACTAAAGTTTTAGCAGACTTAAAAATGGTTGACTTTAGAGAACCTTTTGATAATTTATTAACTCAAGGAATGATTCTTAAAGATGGACTTAAGATGTCTAAATCAAAAGGTAATGTTGTTAGCCCAGATGAAATGATTAAAAAATATGATGCTGATACTATGAGGCTTTTTGTTCTTTTTGCAGCACCGCCTGAAAAAGAACTTGAATGA", "aa": "MHLLYARFFTKVLADLKMVDFREPFDNLLTQGMILKDGLKMSKSKGNVVSPDEMIKKYDADTMRLFVLFAAPPEKELE", "aa_hexdigest": "24ecdb51c27b920da956198c777c61fb", "start_type": "TTG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_UPI002381B5B9", "query_cov": 0.9230769230769231, "subject_cov": 0.8780487804878049, "identity": 0.639, "score": 90.9, "evalue": 1.89e-22, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_UPI002381B5B9" }, "pscc": { "uniref50_id": "UniRef50_UPI002381B5B9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002381B5B9" ], "product": "class I tRNA ligase family protein" }, "genes": [], "id": "OAIKOHHJFB_1178", "locus": "GCA000209735_01164" }, { "type": "cds", "sequence": "contig_1", "start": 644468, "stop": 644575, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAACTTTCAATGCTTATGATAAGATTACTAACTCATTATTAATTAAAGAAATGTACTATGTTTTATTAAACATTTTGGAACCATTTATTCCACATTTAGCTTGA", "aa": "METFNAYDKITNSLLIKEMYYVLLNILEPFIPHLA", "aa_hexdigest": "2aedf62b5f480532fe37bba34bd7b74d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4158.8739000000005, "isoelectric_point": 4.830996131896971 }, "id": "OAIKOHHJFB_1179", "locus": "GCA000209735_01165" }, { "type": "cds", "sequence": "contig_1", "start": 644983, "stop": 645549, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF5D93C", "UniRef:UniRef90_UPI000FF5D93C" ], "nt": "ATGAAAGAAAAAATCTTACTGTTGGACGTCTTAGATAAAGAATATTTATCTGACGCAGTTTATAATCATGAAACAAAAATTTTAGAAATAAAAACAAAAAACCAAAGTTTTAGTGATCAATATTTAAATCACTTGTTTAATTATTTTGAATCAATTAGTCATGATGAAAAGAAAGAAAAAAATGACCAAGCTAAATATTTATTTATTGGTTATTTTGACTACGCAAAAAACAAATATGATGGCAGCGATGAAAGCAGAAAAAAAATAGTTACAAAAAGTAACAATGTAAACAAATATTGTTCTTATGTTAGAGTGCTTGATTTAAATATTGAATACAAACAAGATTCAAAAGAAATCGATAGATATAAACTTAAAGTAAAAGGAATTGGACGTTATAAAATTACTCATTTAGAAGATAATAGTGAAAAAACACCTGAAAATTCATATAATGTTGAATTTGCAGTTGCTTCAAAAAGTTCTTATGAAAATAAATTAAGTAAAGATTCAGTTAAAAAATTAAATGTATTAGATAAAACTATTACAAACTTAGTTGAAAAAGGATTATGA", "aa": "MKEKILLLDVLDKEYLSDAVYNHETKILEIKTKNQSFSDQYLNHLFNYFESISHDEKKEKNDQAKYLFIGYFDYAKNKYDGSDESRKKIVTKSNNVNKYCSYVRVLDLNIEYKQDSKEIDRYKLKVKGIGRYKITHLEDNSEKTPENSYNVEFAVASKSSYENKLSKDSVKKLNVLDKTITNLVEKGL", "aa_hexdigest": "538bdbb1a9a3e4b5b4c67d5328ad9018", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI000FF5D93C", "query_cov": 1.0, "subject_cov": 0.9082125603864735, "identity": 0.9890000000000001, "score": 361.0, "evalue": 9.85e-126, "valid": true, "uniref50_id": "UniRef50_UPI000FF5D93C" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF5D93C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF5D93C" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22128.702400000016, "isoelectric_point": 8.606029701232913 }, "id": "OAIKOHHJFB_1180", "locus": "GCA000209735_01166" }, { "type": "cds", "sequence": "contig_1", "start": 646561, "stop": 647964, "strand": "+", "frame": 1, "gene": null, "product": "endopeptidase La", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P0U7" ], "nt": "ATGTCCTTATTTAAAGAAGATGAAAAAAATGCTAAATTACAAACAGAATTTAACAATGTTCCTATTCTTACTTTAGTTGGTCCTCCAGGTACTGGTAAAACCTCTTTAGCCAGAGCTATTGCTGAAGCGCTTAACAAAAGTTACATTAAAATTAGTCTTGGTGGTGTGCATGATGAAAGTGAAATTAGAGGTCACAGACGTACTTATGTAGGTTCAATGCCAGGTAAAATTATTAAAGCTATCCAAAGAGCTGGTGTATCTAACCCACTTATTTTATTGGATGAAATTGACAAAATGTCATCTGATTATAAAGGTGATCCAGCTAGTGCAATGCTTGAAGTATTAGATCCTGAACAAAACACAAAATTCCAAGATAACTATCTTGAACATGAATATGACTTATCAAAAGTTATGTTTATTGCAACTGCTAACTACTACGAAAATATTCCTGCTCCTTTAATTGACCGTGTGGAAATTATCGAATTAAACTCATATACAATTAATGAAAAAATTAAAATTGCACGTGAACACTTAATTGATTCTACAATTCAACAAGCAGGTCTTAAAAAAGAACAATTCAATATTGATGATAAAACACTAGAATTTATTATCAAACACTATACAGCTGAAGCTGGTGTTCGTGGACTTAAGAGAGATTTAGATAAAATTGCTCGTAAAATAGTTACAAGAATTGTTGAAGGTGAAAAAATTGCTAAATTTACAATAACAAAAGATATTGTTACTGAAATGTTAGGTACTCCTAAATTTACAGATCACCAAGATACGAAAGAACCTCAAGTTGGAGTTGTTAACGGTTTAGCTTACACTTCAATAGGTGGAACAACATTGCAAATCGAAGTTTCAAGCTTCAAGAGTAAAAATAGCGGTATCAAATTAACCGGTTCGCTTAAAGATGTTATGAAGGAATCAGCCAATATTGCTCTTTCATATGTAAGAGCTAATGCTGAAAAATTTGATATTAAAGATTTTGCTTTTGATGAAAGAGAAATTCATATTCACGTTCCAGAAGGAGCTGTTCCTAAAGATGGGCCTAGTGCTGGTGTAACATTTACAACTGCTTTAGTTAGTGCTTTAAGTAATAAACCTGTACCTCAAGATATTGCAATGACAGGTGAAATTACTTTAAGAGGTCGTGTTTTTGAAATTGGTGGATTAAAAGAAAAATCATTTGCTGCATATAAAAAAGGAATCAAAACTGTATTTATTCCAAAAGACAATGAAAAGAATTTAGCAGATATTCCTGAAGAAGTTAAAAAAGCAATTACCTTTATTCCAGTTGAAACTTATGAAGAAATTTATAATCACATTTTTAAAGATCAAAAGCCTACAACTGATAAAAGCGATTCGACTAAAAAGTCTATAAAACAGGCAAATAAGAAATAA", "aa": "MSLFKEDEKNAKLQTEFNNVPILTLVGPPGTGKTSLARAIAEALNKSYIKISLGGVHDESEIRGHRRTYVGSMPGKIIKAIQRAGVSNPLILLDEIDKMSSDYKGDPASAMLEVLDPEQNTKFQDNYLEHEYDLSKVMFIATANYYENIPAPLIDRVEIIELNSYTINEKIKIAREHLIDSTIQQAGLKKEQFNIDDKTLEFIIKHYTAEAGVRGLKRDLDKIARKIVTRIVEGEKIAKFTITKDIVTEMLGTPKFTDHQDTKEPQVGVVNGLAYTSIGGTTLQIEVSSFKSKNSGIKLTGSLKDVMKESANIALSYVRANAEKFDIKDFAFDEREIHIHVPEGAVPKDGPSAGVTFTTALVSALSNKPVPQDIAMTGEITLRGRVFEIGGLKEKSFAAYKKGIKTVFIPKDNEKNLADIPEEVKKAITFIPVETYEEIYNHIFKDQKPTTDKSDSTKKSIKQANKK", "aa_hexdigest": "f6eb224dccc4e6d04b0e4a66fd25e01e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P0U7", "query_cov": 0.9271948608137045, "subject_cov": 0.8694779116465864, "identity": 0.674, "score": 573.0, "evalue": 2.45e-200, "valid": false, "gene": "lon", "product": "endopeptidase La", "ec_ids": [ "3.4.21.53" ], "uniref50_id": "UniRef50_A0A3B0P0U7", "go_ids": [ "GO:0004252", "GO:0005524", "GO:0006508", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P0U7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P0U7" ], "product": "endopeptidase La" }, "genes": [], "id": "OAIKOHHJFB_1181", "locus": "GCA000209735_01167" }, { "type": "cds", "sequence": "contig_1", "start": 648465, "stop": 648734, "strand": "-", "frame": 3, "gene": null, "product": "dUTPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9VF33", "UniRef:UniRef90_C4XFC3" ], "nt": "TTGCACTTTGTTGGCTCTTTTGCTTATCAATATGAAGTTGATCCTAACATCACACCATTAATAATGAGTAAGGATGTTAATAAACAAATTTGCTTATTATTCCAAACAATTTCTTCAGCAACAAATAACATCAATAAATATGTTATTGGAGAAATATTAGCTTTAACTTTAGGGGTTGCTAAATTATTAGGTTACAGTCAAAAAGATCTTTTAGAAGCTTATTACAACAAAAACAAAAAGAATTACGAACGCATTAAAAATAAATATTAG", "aa": "MHFVGSFAYQYEVDPNITPLIMSKDVNKQICLLFQTISSATNNINKYVIGEILALTLGVAKLLGYSQKDLLEAYYNKNKKNYERIKNKY", "aa_hexdigest": "36cb5dfe1e264b4a6a1c29ae44be49fc", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 648465, "stop": 649001, "observations": { "mutation": [ 648791, 648974 ], "stop": [ 648791, 648974 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFC3", "query_cov": 1.0, "subject_cov": 0.5, "identity": 0.9890000000000001, "score": 175.0, "evalue": 1.7e-54, "gene_start": 1, "gene_end": 89, "reference_start": 90, "reference_end": 178, "reference_sequence": "MALLNLLYNWNMNLTKIFTMQKDLDKAIAARNDIDAKTSKSKIKEFHYMKLLALLVETAEFANEVQTFKYWKAKKQIDKDKALEEFADILHFVGSFAYQYEVDPNITPLIMSKDVNKQICLLFQTISSATNNINKYVIGEILALTLGVAKLLGYSQKDLLEAYYNKNKKNYERIKNKY" }, "paralog": false, "identity": 0.9831460674157303, "score": 341.0, "evalue": 2.42e-122, "description": "Internal stop codon at 648791, 648974. Nonsense mutation around 648791, 648974.", "psc": { "uniref90_id": "UniRef90_C4XFC3", "product": "dUTPase", "uniref50_id": "UniRef50_N9VF33", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFC3", "UniRef:UniRef50_N9VF33" ] }, "pscc": { "uniref50_id": "UniRef50_N9VF33", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9VF33" ], "product": "dUTP diphosphatase" } }, "id": "OAIKOHHJFB_1182", "locus": "GCA000209735_01168" }, { "type": "cds", "sequence": "contig_1", "start": 648789, "stop": 648968, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08761.16" ], "nt": "ATGAATTTAACAAAAATATTTACAATGCAAAAAGATCTTGACAAAGCTATTGCAGCTCGTAATGATATTGATGCAAAGACTTCAAAAAGCAAAATTAAAGAATTTCACTACATGAAATTATTAGCTTTACTTGTTGAAACGGCAGAATTTGCAAATGAAGTCCAAACATTCAAATATTGA", "aa": "MNLTKIFTMQKDLDKAIAARNDIDAKTSKSKIKEFHYMKLLALLVETAEFANEVQTFKY", "aa_hexdigest": "c21748c1ffa36f76db06dbac1a3d977f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08761.16", "name": "dUTPase_2", "length": 58, "aa_cov": 0.9830508474576272, "hmm_cov": 0.896551724137931, "evalue": 3.3208196185433197e-13, "score": 46.32384490966797, "start": 2, "stop": 59 } ], "seq_stats": { "molecular_weight": 6855.9497999999985, "isoelectric_point": 9.004702186584474 }, "id": "OAIKOHHJFB_1183", "locus": "GCA000209735_01169" }, { "type": "cds", "sequence": "contig_1", "start": 649025, "stop": 649579, "strand": "-", "frame": 1, "gene": null, "product": "HEAT repeat domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFC4", "UniRef:UniRef90_C4XFC4" ], "nt": "ATGGATGAAGAAAGTGTAGATGCATTATTAAAACGATTAGAAAAAGAAGACAAACTTTATACTAAAATCGCTATTTGTGAAACTTTAGAAACAGGTAATGCTTTTACAGCTAATAAAATGATTGAATATTTGAGCAAAATTGGTAATAACCAAATTAAAGAATTGCCTAAATATATTAGTAATAAAAGTTCTTATCCACTACCAAGAGACATTATTGCCAAATCTTTAGCTAAAATGCATACTTCAATTTTTCCTCTTTTACTTGAAACTCTTAAGCAAGGAACAATTGAACAAAAACAAGAAGTTATTGATGCAATTGGTTACATTTGTTTTAATAAAAAAGAATTAGCTACTGAAAAACTTTTAAATGAGCTAATTGATGTTTATGGTCAAAATATTGATAATAAAATTTTTGTTTGGAAATTTTTAACATTATTATCAGCTTTTAATTTTGAAGCAAGTAAAGAAATACTTAACTCATATTTAAATAGCAAAGATACTTTATCAAAAGAAGCAACTCACTCTTTAGATATATTAAAGGAAGCAAATATTTAG", "aa": "MDEESVDALLKRLEKEDKLYTKIAICETLETGNAFTANKMIEYLSKIGNNQIKELPKYISNKSSYPLPRDIIAKSLAKMHTSIFPLLLETLKQGTIEQKQEVIDAIGYICFNKKELATEKLLNELIDVYGQNIDNKIFVWKFLTLLSAFNFEASKEILNSYLNSKDTLSKEATHSLDILKEANI", "aa_hexdigest": "0537e01ee56c60b62ad7e6c83d2e8095", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 649025, "stop": 649780, "observations": { "mutation": [ 649615, 649711 ], "stop": [ 649615, 649711 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFC4", "query_cov": 1.0, "subject_cov": 0.7330677290836654, "identity": 1.0, "score": 356.0, "evalue": 3.78e-123, "gene_start": 1, "gene_end": 184, "reference_start": 68, "reference_end": 251, "reference_sequence": "MSQIKAHFYIKRILMKPVENKKQWLSRGYVDQEDIAEFENKSKKELLELLQHEYAWVRSSAAYNLSPMDEESVDALLKRLEKEDKLYTKIAICETLETGNAFTANKMIEYLSKIGNNQIKELPKYISNKSSYPLPRDIIAKSLAKMHTSIFPLLLETLKQGTIEQKQEVIDAIGYICFNKKELATEKLLNELIDVYGQNIDNKIFVWKFLTLLSAFNFEASKEILNSYLNSKDTLSKEATHSLDILKEANI" }, "paralog": false, "identity": 0.9920318725099602, "score": 482.0, "evalue": 2.41e-175, "description": "Internal stop codon at 649615, 649711. Nonsense mutation around 649615, 649711.", "psc": { "uniref90_id": "UniRef90_C4XFC4", "product": "HEAT repeat domain-containing protein", "uniref50_id": "UniRef50_C4XFC4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFC4", "UniRef:UniRef50_C4XFC4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFC4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFC4" ], "product": "HEAT repeat domain-containing protein" } }, "id": "OAIKOHHJFB_1184", "locus": "GCA000209735_01170" }, { "type": "cds", "sequence": "contig_1", "start": 650172, "stop": 650270, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGGAGATCTATGTCAACAGAAAACAAAACAGAATCTTTAAACAAAGTAAACAATGAAAAATTGGAAGCAAAAGAATTAAGCTATACTAAATGA", "aa": "MKRRSMSTENKTESLNKVNNEKLEAKELSYTK", "aa_hexdigest": "5d5edf307d0e1169c7525b30cfa8f4ef", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3788.2687000000014, "isoelectric_point": 9.600390052795408 }, "id": "OAIKOHHJFB_1185", "locus": "GCA000209735_01171" }, { "type": "cds", "sequence": "contig_1", "start": 650384, "stop": 650527, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAATCTTAATTATAGGATTCTTCGTTGGAATATTAACATTCATCGGAGCATTTATTTATATTTCTGATGCTCAAAAAGAAAATAAAAACTACTTCAATTATTTTAAATCAGATGAATCAATTGACTCAAAAGCAGAATAG", "aa": "MIILIIGFFVGILTFIGAFIYISDAQKENKNYFNYFKSDESIDSKAE", "aa_hexdigest": "33a6840712a18ef3e6ea85f4642cdb96", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5415.169400000001, "isoelectric_point": 4.748352241516113 }, "id": "OAIKOHHJFB_1186", "locus": "GCA000209735_01172" }, { "type": "cds", "sequence": "contig_1", "start": 650645, "stop": 650803, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATAGAAAAAATCCTAAAAGTTCAGCTATTGCTTTGATTGTTTTATCACTTTTAAGCGCTGTTATAACAGGAATTGCAATATTTATGTTTATTGATGTTGCAGTTAAAATATTTAAAAGTGGAGAAGAAACAAATGAATATATTAAAAATAACTGA", "aa": "MHRKNPKSSAIALIVLSLLSAVITGIAIFMFIDVAVKIFKSGEETNEYIKNN", "aa_hexdigest": "9c018b39b53836b8fc329e9044cc8e2f", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5736.745, "isoelectric_point": 9.302868461608885 }, "id": "OAIKOHHJFB_1187", "locus": "GCA000209735_01173" }, { "type": "cds", "sequence": "contig_1", "start": 651465, "stop": 651662, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGATTAAATTAGCATCTATTTCTAAAGCTTTTTCGAAAACTTCTTTGGTTAAGTCAATAGCACAAACTATACTACGTAAAGGTTCTGATAAATTGAATTTAATAGAAAAACCACTTGGGTCTCATTCTTCTTTAAGATCAAGTGGTCACTTTTCTAATAAATATTTTGTTACTTTTCTAATTTGCATTATTTAA", "aa": "MMIKLASISKAFSKTSLVKSIAQTILRKGSDKLNLIEKPLGSHSSLRSSGHFSNKYFVTFLICII", "aa_hexdigest": "e26b4431c9406e3ce81c146386714cbd", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7186.508499999996, "isoelectric_point": 10.302902030944821 }, "id": "OAIKOHHJFB_1188", "locus": "GCA000209735_01174" }, { "type": "cds", "sequence": "contig_1", "start": 651655, "stop": 652158, "strand": "-", "frame": 2, "gene": null, "product": "sigma-70 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF345E0" ], "nt": "ATGGTTGAAACTATTAACAAAATCACTAAAATTGAACGTGAATTGCAACAAGAATTAGGTTATGAACCAACTGATGAAATGATTGCTGAAAGATATGGCAATGGCTATGACGCTGAAAAAGTTAGATACATTAGAAAAATCAACATTGACCCTATTTCTTTAGACAAACAAATTGGTAAAGAAAATGATTCATCATTTAGTGACTTTGTTAAAGATGAAAGTGTTGTTAATCCAGTTGATTATGCTTCACAAGAAGAATTAGTGGAAACACTAAATTCAATTCTTGAAAAATCACTTGATGATGATGAAAGAATTTTAATCTGCAAACGTTTCGGAGTTGGAGTTGACGAAAATGGAAATAAATACCCAATTCACTCACTTGAAAAATTAGCAGAAGAAAGAAATGTTTCTAAAGAAAGAATTCGTCAAATTGAAAACAAAATTTTAAGAAAACTTAAAAGTAGCACACGTAAAGGACGTCACTTGAAAGATTTCATTAAATAA", "aa": "MVETINKITKIERELQQELGYEPTDEMIAERYGNGYDAEKVRYIRKINIDPISLDKQIGKENDSSFSDFVKDESVVNPVDYASQEELVETLNSILEKSLDDDERILICKRFGVGVDENGNKYPIHSLEKLAEERNVSKERIRQIENKILRKLKSSTRKGRHLKDFIK", "aa_hexdigest": "d8910b176a042d5196fe307d3e10f9a7", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI002FF345E0", "query_cov": 0.8922155688622755, "subject_cov": 0.9933333333333333, "identity": 0.591, "score": 181.0, "evalue": 8.13e-56, "valid": false, "product": "sigma-70 domain-containing protein", "uniref50_id": "UniRef50_UPI002FF345E0" }, "pscc": { "uniref50_id": "UniRef50_UPI002FF345E0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF345E0" ], "product": "sigma-70 domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_1189", "locus": "GCA000209735_01175" }, { "type": "cds", "sequence": "contig_1", "start": 652225, "stop": 652431, "strand": "-", "frame": 2, "gene": null, "product": "sigma-70 family RNA polymerase sigma factor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021E0C4F6", "UniRef:UniRef90_UPI0021E0C4F6" ], "nt": "ATGGAAAAAGGTGGCTTTAGAGGAAAACGTGCTAGGGATAAACTTATCAAACGTAATTTACGTTTAGTTATCAATAATGCTAAAAAATACAAAAATAGAGGACTTAGCTTTATTGACTTAATCTCAGAAGGTAACTCAGGTATCGTAAAAGCAGTTTCAAAATATAACGTTTCAAAAGGCTTTAAATTCTCAACATATGCAACTTGA", "aa": "MEKGGFRGKRARDKLIKRNLRLVINNAKKYKNRGLSFIDLISEGNSGIVKAVSKYNVSKGFKFSTYAT", "aa_hexdigest": "ed6a4481227ac60f778d6860788d7ef0", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 652153, "stop": 652477, "observations": { "insertion": [ 652435 ], "mutation": [ 652459 ], "stop": [ 652224, 652227, 652459 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0021E0C4F6", "query_cov": 1.0, "subject_cov": 0.591304347826087, "identity": 0.868, "score": 117.0, "evalue": 7.01e-33, "gene_start": 1, "gene_end": 68, "reference_start": 19, "reference_end": 86, "reference_sequence": "MERWKTSRWCWRKKLCNEIEKGGFRGKRARDKLVLRNLRLVINNAKKYKNRGLSFIDLISEGNQGIMKAVQKFDVSKGYKFSTYATWWIRQAITRAVADQARTIRVYLYIYRNNK" }, "paralog": false, "identity": 0.7850467289719626, "score": 159.0, "evalue": 1.17e-51, "description": "Internal stop codon at 652224, 652227, 652459. Frameshift due to insertion around 652435. Nonsense mutation around 652459.", "psc": { "uniref90_id": "UniRef90_UPI0021E0C4F6", "product": "sigma-70 family RNA polymerase sigma factor", "uniref50_id": "UniRef50_UPI0021E0C4F6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0021E0C4F6", "UniRef:UniRef50_UPI0021E0C4F6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0021E0C4F6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021E0C4F6" ], "product": "sigma-70 family RNA polymerase sigma factor" } }, "id": "OAIKOHHJFB_1190", "locus": "GCA000209735_01176" }, { "type": "cds", "sequence": "contig_1", "start": 652501, "stop": 653124, "strand": "-", "frame": 2, "gene": null, "product": "RNA polymerase sigma factor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFC8", "UniRef:UniRef90_C4XFC8" ], "nt": "ATGAATAAAGCAAATGAAATTAAATCAATAATCTCAATGATTAAAAAACATGCAAAAAGCTTAAAGAAAAAACAAATAAGTCAGTCTGAAGTTTATGATTATTTAGATTCAATTAAAGTTGAAATTGATGATGAATCAATGGACGAAGTTTTAGGCCAACTTTTTGAATCAGGCTTAATTCTTGATGAAAGCGACGAGGGCGATTTAGACGATGTTAGTTTAGATGAATTAGACTCAGAATTAGAATTCGAAGAAAAAAGCAAAAAAGACGAAAACACCGATGAAATGGATGTTAAAAACAAAAAAAGTGATTTATATGACGAAGATTTAGATGAAGATGATAACTACAATTTAGTTGGTTCATATGGTGACGATGATGAAAGTCAAGAATACATTATTGAAGATGACTATGTTGATTCAAGTTATGATGAAGATGACGAAGATGAAGACGAAGAAGAATTAAGCAGTAAGAAAAAATCGAAAAAAAGTAAAAATAATGAAGATGAAGATTCACTTGAACTTGAATCTTATGAAAATGAAGAAATTAACCTTAATGATAACAAAGTAATTAAAGATAAAGATTTAACCAACAAACTTACCGAAACTAATGATATTGTTAAATGA", "aa": "MNKANEIKSIISMIKKHAKSLKKKQISQSEVYDYLDSIKVEIDDESMDEVLGQLFESGLILDESDEGDLDDVSLDELDSELEFEEKSKKDENTDEMDVKNKKSDLYDEDLDEDDNYNLVGSYGDDDESQEYIIEDDYVDSSYDEDDEDEDEEELSSKKKSKKSKNNEDEDSLELESYENEEINLNDNKVIKDKDLTNKLTETNDIVK", "aa_hexdigest": "250567412293912e59135d44f9265a0f", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 652198, "stop": 653124, "observations": { "mutation": [ 652224, 652227, 652491, 652503 ], "stop": [ 652224, 652227, 652491, 652503 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFC8", "query_cov": 1.0, "subject_cov": 0.4233128834355828, "identity": 1.0, "score": 308.0, "evalue": 9.79e-101, "gene_start": 1, "gene_end": 207, "reference_start": 1, "reference_end": 207, "reference_sequence": "MNKANEIKSIISMIKKHAKSLKKKQISQSEVYDYLDSIKVEIDDESMDEVLGQLFESGLILDESDEGDLDDVSLDELDSELEFEEKSKKDENTDEMDVKNKKSDLYDEDLDEDDNYNLVGSYGDDDESQEYIIEDDYVDSSYDEDDEDEDEEELSSKKKSKKSKNNEDEDSLELESYENEEINLNDNKVIKDKDLTNKLTETNDIVKWYMRWIGKYGKLLTIEEEKKLAQEMEKGGFRGKRARDKLIKRNLRLVINNAKKYKNRGLSFIDLISEGNSGIVKAVSKYNVSKGFKFSTYATWWIRQAITRAVADQARTIRVPVHMVETINKITKIERELQQELGYEPTDEMIAERYGNGYDAEKVRYIRKINIDPISLDKQIGKENDSSFSDFVKDESVVNPVDYASQEELVETLNSILEKSLDDDERILICKRFGVGVDENGNKYPIHSLEKLAEERNVSKERIRQIENKILRKLKSSTRKGRHLKDFIK" }, "paralog": false, "identity": 0.987012987012987, "score": 525.0, "evalue": 3.83e-188, "description": "Internal stop codon at 652224, 652227, 652491, 652503. Nonsense mutation around 652224, 652227, 652491, 652503.", "psc": { "uniref90_id": "UniRef90_C4XFC8", "product": "RNA polymerase sigma factor", "uniref50_id": "UniRef50_C4XFC8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFC8", "UniRef:UniRef50_C4XFC8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFC8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFC8" ], "product": "RNA polymerase sigma factor" } }, "id": "OAIKOHHJFB_1191", "locus": "GCA000209735_01177" }, { "type": "cds", "sequence": "contig_1", "start": 653117, "stop": 653467, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTCGGTAAATTAAATAATGAAGATTTAAAAAAAGAACTTTATCAAACATTTAGAGAAAGTAAAAGTATTAAGGTTGCTAAATTAAAGGAATATATCAAAGACTTAGAACAGGAAAATGTTGATATCGATTTAAATCAATTTTCAAAAAAATACAAAGAGGATAAAATTATTAAACAAAACTTTAAAGATGTTTATCGCCGAGCATTAAAGGAATCAGATAATGCTTATAATAAATACACAAGCAATCCTGAAGTTTTGAAACAAATCAGCAGTATCAAAAAAATGTATGATGAACACAAAGAAAAATTAAGAGAAATGCAAAAAAGAAAGGAAAAAAATGAATAA", "aa": "MFFGKLNNEDLKKELYQTFRESKSIKVAKLKEYIKDLEQENVDIDLNQFSKKYKEDKIIKQNFKDVYRRALKESDNAYNKYTSNPEVLKQISSIKKMYDEHKEKLREMQKRKEKNE", "aa_hexdigest": "64c6d18f5ecdff00593736bf46e57e19", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14194.076199999985, "isoelectric_point": 9.379585838317869 }, "id": "OAIKOHHJFB_1192", "locus": "GCA000209735_01178" }, { "type": "cds", "sequence": "contig_1", "start": 653552, "stop": 654187, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13662.12" ], "nt": "ATGGGTACAGCTTTAACCAATAATCATTTCCGATTACTAAAAAACAAAAAAGTAGTCATCTTTTTAGATGGTGATGATGCTGGTCAAAAAGCTTCACTAAAAAGTGCTAAGTTTTTATTAGACCATCGTATTGACACTTACATAGTACGTAACACAACTAAATATGACCCCGATGAATTATTTAATAGTTATGGTGCCGATATTCTTCACAAGTTAATTAAAGAAGCTCCAGCTGCATTAGATTTTATTTATGAAAATTTAAGCAAGAAATATAATCTCAGTGTTTCGCCAAACAATGAAATCAAAAATGTTAAAGCTTTTTGCAATGACTTTGTTAACTTCCTTGAACACCAAGAAATTAATGTTCAAGAATTCTACAAAAACAAAATTCAAACCGATTATGGTTTTAGCATTCAAGTAAAACACAACTTTAAGTTGCCTGATCCTTATGATTCTCAAGAAATTTATGCACCACAACAATTTCCAGAAGACTCAGGTGAATATCAATTATTAGAAAACAATATTCCCCCTTATGCTTATGATGAACAAAATTATAATCAAGTAGTTGAATACAGCAATAATGTCAATAATTTTAATCACCCTTTAAATAACGAAGTGCCATTAAGCGATTATTGA", "aa": "MGTALTNNHFRLLKNKKVVIFLDGDDAGQKASLKSAKFLLDHRIDTYIVRNTTKYDPDELFNSYGADILHKLIKEAPAALDFIYENLSKKYNLSVSPNNEIKNVKAFCNDFVNFLEHQEINVQEFYKNKIQTDYGFSIQVKHNFKLPDPYDSQEIYAPQQFPEDSGEYQLLENNIPPYAYDEQNYNQVVEYSNNVNNFNHPLNNEVPLSDY", "aa_hexdigest": "b73073e19d5197eec1d296505d8a2b58", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13662.12", "name": "Toprim_4", "length": 56, "aa_cov": 0.22274881516587677, "hmm_cov": 1.0, "evalue": 2.3672943462283753e-07, "score": 27.045278549194336, "start": 1, "stop": 47 } ], "seq_stats": { "molecular_weight": 24562.979999999996, "isoelectric_point": 5.014642906188963 }, "id": "OAIKOHHJFB_1193", "locus": "GCA000209735_01179" }, { "type": "cds", "sequence": "contig_1", "start": 654788, "stop": 655024, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01807.25" ], "nt": "ATGAGTAATAATAACCATGAAATAGTTGACTCAATTATTGCAAGTAATGATATTGTTGATGTTATCAACTCATTTGTTACTTTAAGCAAAAAAGGTCGTAGTTTTGTTTGCATTTGTCCTTTTCACCAAGACACAAATCCATCAATGAATGTCGACCCTAAAAAGCAAATATTTAAATGCTTTGTCTGCCAAGTTGGTGGTAATGTTTTAACATTTTTAATGAAATACAAAAAATGA", "aa": "MSNNNHEIVDSIIASNDIVDVINSFVTLSKKGRSFVCICPFHQDTNPSMNVDPKKQIFKCFVCQVGGNVLTFLMKYKK", "aa_hexdigest": "279c97a7e20988a35d5eda3ed4f4a468", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01807.25", "name": "Zn_ribbon_DnaG", "length": 73, "aa_cov": 0.9358974358974359, "hmm_cov": 1.0, "evalue": 1.2669441797049248e-28, "score": 94.85169219970703, "start": 6, "stop": 78 } ], "seq_stats": { "molecular_weight": 8798.178199999998, "isoelectric_point": 8.781061363220214 }, "id": "OAIKOHHJFB_1194", "locus": "GCA000209735_01180" }, { "type": "cds", "sequence": "contig_1", "start": 655058, "stop": 655516, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25" ], "nt": "ATGAAAGCTACTGGTGAAAACTTAGAATATGTTGACCCTGTAACAAACGAAAAATTTGTTCCTTTTGTTATTGAACCTTCAATAGGACTTGACCGTTTAATGTTAGCAATTTTAATTGATTCATATGACGAAGAAAAACTTGAAAAAGATGACTCAAGAGTTGTTTTACATCTACACAAAAATTTAGCTCCTTATCAAGTTGCAGTTTTACCTTTAGTTAAAAAACTAAATGATAAAGCAACAGAAGTTTATGAAAAATTACTAAAAGCAGGCTTATCTGTAACTTATGATGAAACTGGATCAATAGGCAAAAGATACCGTCGTCAAGATGCTATTGGTACACCTTATTGTGTTACAATAGACTTTGATTCACTTGAAAAAAATACTGTAACAATTAGAGACAGAGACAGTATGAAACAAAAAACTGTACTAATTAATAATTTACTAAAAGAACTATAA", "aa": "MKATGENLEYVDPVTNEKFVPFVIEPSIGLDRLMLAILIDSYDEEKLEKDDSRVVLHLHKNLAPYQVAVLPLVKKLNDKATEVYEKLLKAGLSVTYDETGSIGKRYRRQDAIGTPYCVTIDFDSLEKNTVTIRDRDSMKQKTVLINNLLKEL", "aa_hexdigest": "f97b55e36458c04480f1374f6be2bb55", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P9T9", "query_cov": 0.993421052631579, "subject_cov": 0.8830409356725146, "identity": 0.57, "score": 173.0, "evalue": 9.79e-53, "valid": false, "product": "Glycine--tRNA ligase", "uniref50_id": "UniRef50_A0A3B0P9T9" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 91, "aa_cov": 0.5723684210526315, "hmm_cov": 1.0, "evalue": 2.2914471302056905e-27, "score": 91.13040924072266, "start": 66, "stop": 152 } ], "seq_stats": { "molecular_weight": 17329.781799999997, "isoelectric_point": 5.477766990661621 }, "id": "OAIKOHHJFB_1195", "locus": "GCA000209735_01181" }, { "type": "cds", "sequence": "contig_1", "start": 655568, "stop": 655972, "strand": "-", "frame": 1, "gene": null, "product": "aminoacyl--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0017CB9A86" ], "nt": "ATGTTTGAAACCAAACAAGGAGTTACAGAAGAAACCAAAAACAAAATTTACTTACGTCCTGAAACTGCTCAAGGTATTTTTGTTAACTTTAAAAACGTACAAAGAAGCATGCGTTCAAAATTACCTATGGGCATAGGGCAAGTTGGAAAAAGTTTCAGAAATGAAGTAACACCAGGTAACTTTATTTTCCGTACTCGTGAATTTGAACAAATGGAACTTGAAGTATTTTGCCATCCAAAAGAAGCAAACAAACTTTACAAATACTACATTAAAAAAGCTGAAAAATTTGCTGAATATTTAGGCTTAAGCAAAGAAAATGTTCGTATCAGAGAACATGAAAAAGAAGAACTAAGTCATTATTCTTCAGGCACAAGTGACATTGAATATCTTTTCCCTTTTGGCTGA", "aa": "MFETKQGVTEETKNKIYLRPETAQGIFVNFKNVQRSMRSKLPMGIGQVGKSFRNEVTPGNFIFRTREFEQMELEVFCHPKEANKLYKYYIKKAEKFAEYLGLSKENVRIREHEKEELSHYSSGTSDIEYLFPFG", "aa_hexdigest": "8d4028caa58c5e3eefa13a1b12699004", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0017CB9A86", "query_cov": 0.8582089552238806, "subject_cov": 0.8273381294964028, "identity": 0.6, "score": 145.0, "evalue": 3.18e-42, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "uniref50_id": "UniRef50_UPI0017CB9A86" }, "pscc": { "uniref50_id": "UniRef50_UPI0017CB9A86", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0017CB9A86" ], "product": "aminoacyl--tRNA ligase-related protein" }, "genes": [], "id": "OAIKOHHJFB_1196", "locus": "GCA000209735_01182" }, { "type": "cds", "sequence": "contig_1", "start": 656315, "stop": 656419, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAATAATGAAAAAAATATGACTACTTTAATAAATCATTTAAAAGCTAGTGGATTTGTTTATCAAGGTAGTGAAATTTATGGTGGTTTAGCTAATACTTGA", "aa": "MKNNEKNMTTLINHLKASGFVYQGSEIYGGLANT", "aa_hexdigest": "051db83d179e4d9698cbf2e553135eee", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3745.2002, "isoelectric_point": 8.161391258239746 }, "id": "OAIKOHHJFB_1197", "locus": "GCA000209735_01183" }, { "type": "cds", "sequence": "contig_1", "start": 656499, "stop": 657068, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGAAGCTGTTTTAGCTGCTGAAAAATCTTCTAAAACTAAAAAACAACCTAAAACTGAAACTATAGTTACTGAAGTGCCTCCAGTAGTTGAAGAAGTTAAAGATGAATCAGTTGTTGAAGCTGAAGCTGTAGTTGAAGAAGTTAAACCAGTTGAAGAAAAACAACCAACAATTAAAGAAGAACCGCTTATTGTTTTAGCTCCTGAAGAAGCTAAAAAAGAAAAAGTTGAAGACAATGATTTTGTCTTAATTGACCATAAAAAAGCTGAAAAACCTAAAACAAGAGACTTTGCTGTTAGAGTTGTTGAAGAAAAACCTATTCAAGTAATTGAAGAAAAAGAAGCTCAACCTGAAGATAATTTCTACACAGGAATTAGAGGTAATGAACCAGTTGCTCTAGAATCAGAAGAATCATCAAACAATGTTTCAACTAAGAAAAAACGTTCAGCAGGATTCTACTTCTTAATCTTATTATTAGTACTAATTCTTGCAGGTCTTTTAACCTTAGATATTCTTGCTATTATTCAATTAGCAGGTGGTTATACATTCTTTAATTTTGGTGCTTAA", "aa": "MEEAVLAAEKSSKTKKQPKTETIVTEVPPVVEEVKDESVVEAEAVVEEVKPVEEKQPTIKEEPLIVLAPEEAKKEKVEDNDFVLIDHKKAEKPKTRDFAVRVVEEKPIQVIEEKEAQPEDNFYTGIRGNEPVALESEESSNNVSTKKKRSAGFYFLILLLVLILAGLLTLDILAIIQLAGGYTFFNFGA", "aa_hexdigest": "bcf4e3f85d9e51ff9a607d5f6a1ffabb", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21064.854000000007, "isoelectric_point": 4.723740959167479 }, "id": "OAIKOHHJFB_1198", "locus": "GCA000209735_01184" }, { "type": "cds", "sequence": "contig_1", "start": 657243, "stop": 657662, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTCAATGAAATGGCTCGTAAAATGGAACTTTTAAGACAAAACTACAAGCCACTTGAAGGTGTAACATACATGAACTACATCACATTAAGTTTTGATGACTATGTAGGTGGTGTTGCTTTATACACAGAAAAAATTAAAAATATTATTGAAACAACTCCAAAACAAAATATTAGTGAAGCTGATTACAATAATATTAAGAAAAACTATGCTAACTTCAATAAACAATTATTAGAAGTTGAAGCAAATCCTAACTTCCCACATGCTGATTTAGTTCAAAAAATGCATGCTGAATTAAGTACAACTTTTGAATCATTACTTAGTTTATTAGTCTTAGATCCTAAATTAGAAGATACACCAGCTAACCGCGGTTATTTCCTAAATAAAGAAACTAAGCAAAAACAAGAAATCTCATGA", "aa": "MNFNEMARKMELLRQNYKPLEGVTYMNYITLSFDDYVGGVALYTEKIKNIIETTPKQNISEADYNNIKKNYANFNKQLLEVEANPNFPHADLVQKMHAELSTTFESLLSLLVLDPKLEDTPANRGYFLNKETKQKQEIS", "aa_hexdigest": "573070d8fb7684ef898c1a37d418e986", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16130.149199999983, "isoelectric_point": 5.434739875793456 }, "id": "OAIKOHHJFB_1199", "locus": "GCA000209735_01185" }, { "type": "cds", "sequence": "contig_1", "start": 658403, "stop": 658627, "strand": "+", "frame": 2, "gene": null, "product": "S1 motif domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFD2", "UniRef:UniRef90_C4XFD2" ], "nt": "TTGACCAAAAAAATTGCTAAAAAATCAAATATTTTTGCATTTTTTCTTATTTTTTTCCATTTAATTATTAATATAAACATAAAGTTTAAGTATAATAAAAACACCATGAATCATAAAGGCGATATTTTATTCGGAAAAATAAAAAGTGTAAGTAGTGAAGGACTTACAATTTTATGCAATAATGATTATTCTTATTTTATTCCTCGTGATTTGATAACTGACTGA", "aa": "MTKKIAKKSNIFAFFLIFFHLIININIKFKYNKNTMNHKGDILFGKIKSVSSEGLTILCNNDYSYFIPRDLITD", "aa_hexdigest": "3302a32d0eb16ac32954932f9fb56dd5", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 658361, "stop": 658867, "observations": { "mutation": [ 658625 ], "stop": [ 658625 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFD2", "query_cov": 1.0, "subject_cov": 0.44047619047619047, "identity": 0.986, "score": 149.0, "evalue": 1.03e-44, "gene_start": 1, "gene_end": 74, "reference_start": 15, "reference_end": 88, "reference_sequence": "MSSKKLAKNKYFLFLTKKIAKKSNIFAFFLIFFHLIININIKFKYNKNTMNHKGDILFGKIKSVSSEGLTILCNNDYSYFIPRDLITDWNKINLRHEFKIGEKINFIVEKVDYHSHSGIGNFKINHATFSRSPFKENLSNTKHGFENLKNQIDQEIKNYHKEGNNGNN" }, "paralog": false, "identity": 0.9880952380952381, "score": 341.0, "evalue": 1.37e-122, "description": "Internal stop codon at 658625. Nonsense mutation around 658625.", "psc": { "uniref90_id": "UniRef90_C4XFD2", "product": "S1 motif domain-containing protein", "uniref50_id": "UniRef50_C4XFD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFD2", "UniRef:UniRef50_C4XFD2" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFD2" ], "product": "S1 motif domain-containing protein" } }, "id": "OAIKOHHJFB_1200", "locus": "GCA000209735_01186" }, { "type": "cds", "sequence": "contig_1", "start": 658640, "stop": 658867, "strand": "+", "frame": 2, "gene": null, "product": "S1 motif domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFD2", "UniRef:UniRef90_C4XFD2" ], "nt": "TTGCGTCATGAATTTAAGATCGGCGAAAAAATTAATTTTATAGTTGAAAAAGTTGATTATCACAGTCATAGTGGTATTGGTAATTTTAAAATTAATCATGCCACCTTTTCACGCAGTCCATTTAAAGAAAATTTAAGCAATACAAAACATGGTTTTGAAAACTTGAAAAACCAAATTGATCAAGAAATCAAAAATTATCATAAAGAAGGTAATAATGGCAACAATTAA", "aa": "MRHEFKIGEKINFIVEKVDYHSHSGIGNFKINHATFSRSPFKENLSNTKHGFENLKNQIDQEIKNYHKEGNNGNN", "aa_hexdigest": "8887cc25eed6748d55227d3c5a208f77", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 658361, "stop": 658867, "observations": { "mutation": [ 658625 ], "stop": [ 658625 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFD2", "query_cov": 1.0, "subject_cov": 0.44642857142857145, "identity": 0.987, "score": 149.0, "evalue": 1.53e-44, "gene_start": 1, "gene_end": 75, "reference_start": 94, "reference_end": 168, "reference_sequence": "MSSKKLAKNKYFLFLTKKIAKKSNIFAFFLIFFHLIININIKFKYNKNTMNHKGDILFGKIKSVSSEGLTILCNNDYSYFIPRDLITDWNKINLRHEFKIGEKINFIVEKVDYHSHSGIGNFKINHATFSRSPFKENLSNTKHGFENLKNQIDQEIKNYHKEGNNGNN" }, "paralog": false, "identity": 0.9880952380952381, "score": 341.0, "evalue": 1.42e-122, "description": "Internal stop codon at 658625. Nonsense mutation around 658625.", "psc": { "uniref90_id": "UniRef90_C4XFD2", "product": "S1 motif domain-containing protein", "uniref50_id": "UniRef50_C4XFD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFD2", "UniRef:UniRef50_C4XFD2" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFD2" ], "product": "S1 motif domain-containing protein" } }, "id": "OAIKOHHJFB_1201", "locus": "GCA000209735_01187" }, { "type": "cds", "sequence": "contig_1", "start": 658854, "stop": 659294, "strand": "+", "frame": 3, "gene": null, "product": "Glucose-6-phosphate isomerase", "db_xrefs": [ "RefSeq:WP_015511063.1", "SO:0001217", "UniParc:UPI0001A8C30E", "UniRef:UniRef100_C4XFD3", "UniRef:UniRef50_A0A3P8LHM8", "UniRef:UniRef90_C4XFD3" ], "nt": "ATGGCAACAATTAATTTGAAAGCTTATAATTATTCACCTGATTTTAAAAATGAAAGTTTGATTAATCGTGGCAATAACTTGATTAAGGATGTTAAATTAAAAAATATCGAGGGTTTTGAACATTTTGGTTTTCATGAATTAGCTTTAAATTTTAGTAATTTTAATGTTGATGAATTAAACAATTTTGTACGCTTAATAAAAAAGCAAGAAATTAAAGAAATTTTGATTATGACAACAAGCAAAATAATTGATAATTATCAAGCTGGAAATGACTTTTTATTTAAGTATGATTTATTAAAAAAATACAAGTTAAAATTTAATTTTATTAATGTTGATAATTATGAGCAAGATTACTATGCTAAGTTTGAAGAGTATAAGAAAAAATTTGAACAAAAAGGCATTGCTTTAATGTTTGGATCATTTACTAAATTTAGTGAATAA", "aa": "MATINLKAYNYSPDFKNESLINRGNNLIKDVKLKNIEGFEHFGFHELALNFSNFNVDELNNFVRLIKKQEIKEILIMTTSKIIDNYQAGNDFLFKYDLLKKYKLKFNFINVDNYEQDYYAKFEEYKKKFEQKGIALMFGSFTKFSE", "aa_hexdigest": "10d0b05a24399f7b04ed3e43ab80774c", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI0001A8C30E", "ncbi_nrp_id": "WP_015511063.1", "uniref100_id": "UniRef100_C4XFD3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C30E", "RefSeq:WP_015511063.1", "UniRef:UniRef100_C4XFD3" ] }, "ips": { "uniref100_id": "UniRef100_C4XFD3", "uniref90_id": "UniRef90_C4XFD3" }, "psc": { "uniref90_id": "UniRef90_C4XFD3", "uniref50_id": "UniRef50_A0A3P8LHM8" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LHM8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LHM8" ], "product": "Glucose-6-phosphate isomerase" }, "genes": [], "id": "OAIKOHHJFB_1202", "locus": "GCA000209735_01188" }, { "type": "cds", "sequence": "contig_1", "start": 659319, "stop": 659564, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCATTTTCAATTAAATCAAGGTTATTATCGAGCACTTGAAAGAGTTTTTTTAATCTCAAAAGAATCATTAGAAAAACAATTGGAATTTTTAAGTATTCCCCTTCAAAACAGACTTATTAGTCCAAATATCCTCTCAAATAATTTTGCTTTTTTTAGTGAAATTAATATGTTACTTTTGTTGCTTCAAGGAGCTAATATTAATGACTTATCTGAAGGCTATTCTTCAGGTTGCACAAACTGA", "aa": "MNHFQLNQGYYRALERVFLISKESLEKQLEFLSIPLQNRLISPNILSNNFAFFSEINMLLLLLQGANINDLSEGYSSGCTN", "aa_hexdigest": "d2087bb19412d48c5d7bab6746862451", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9285.50649999999, "isoelectric_point": 5.130821800231933 }, "id": "OAIKOHHJFB_1203", "locus": "GCA000209735_01189" }, { "type": "cds", "sequence": "contig_1", "start": 659682, "stop": 660047, "strand": "+", "frame": 3, "gene": null, "product": "Glucose-6-phosphate isomerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFD4", "UniRef:UniRef90_C4XFD4" ], "nt": "TTGAAGCTTTATAGCACAATAAATAATAGTCAATATTTAAAGTCAAATAATGTGACAATGTATGCAATTTTTAGTCAAGATATTTATACTTATGGACAATATTTAATTGATAATAATCGCAACTTTTTTACCTCAATTTTTAGCATTGAAAATGAAAAAATTGACTTTAGAATTAGTGATGAAATTAATAATACTCAAGATGGTTTAGAGAACCATAAAGATAACCGCATAAGCAGCTATTACAAAGTAGCAAAAAAAGGTTATTTGACAACACTTTCAAATGTTGCATCATTACCAGTTTTTGATATTTCATTGGAAAGTTCAAATGAATTAAATTTAGGATACTTAGTTGGATTTCTATATTGA", "aa": "MKLYSTINNSQYLKSNNVTMYAIFSQDIYTYGQYLIDNNRNFFTSIFSIENEKIDFRISDEINNTQDGLENHKDNRISSYYKVAKKGYLTTLSNVASLPVFDISLESSNELNLGYLVGFLY", "aa_hexdigest": "cc5ee58e5096f7b663262de3093daad5", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 659382, "stop": 660098, "observations": { "mutation": [ 659562, 660045 ], "stop": [ 659562, 660045 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFD4", "query_cov": 1.0, "subject_cov": 0.4548872180451128, "identity": 0.992, "score": 236.0, "evalue": 9.96e-77, "gene_start": 1, "gene_end": 121, "reference_start": 129, "reference_end": 249, "reference_sequence": "MNFIKLLMNHFQLNQGYYRALERVFLISKESLEKQLEFLSIPLQNRLISPNILSNNFAFFSEINMLLLLLQGANINDLSEGYSSGCTNWLSSNLEQNKAFQYAYIRYSLKGDRKNDLLIGTDERFKGILKLYSTINNSQYLKSNNVTMYAIFSQDIYTYGQYLIDNNRNFFTSIFSIENEKIDFRISDEINNTQDGLENHKDNRISSYYKVAKKGYLTTLSNVASLPVFDISLESSNELNLGYLVGFLYWSYIFECYLNKLNPFDI" }, "paralog": false, "identity": 0.9915966386554622, "score": 466.0, "evalue": 6.44e-170, "description": "Internal stop codon at 659562, 660045. Nonsense mutation around 659562, 660045.", "psc": { "uniref90_id": "UniRef90_C4XFD4", "product": "Glucose-6-phosphate isomerase", "uniref50_id": "UniRef50_C4XFD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFD4", "UniRef:UniRef50_C4XFD4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFD4" ], "product": "Glucose-6-phosphate isomerase" } }, "id": "OAIKOHHJFB_1204", "locus": "GCA000209735_01190" }, { "type": "cds", "sequence": "contig_1", "start": 660207, "stop": 660329, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGCAGTTAAAGTTAATTTACATAATTGTGTTGATGAAAAAACATTACTTTCTTATCAAGACAAAGTTAAACAAATTCATGATTCAATAATGAACAAAACTATTGCAGAAAAAGATTGA", "aa": "MKAVKVNLHNCVDEKTLLSYQDKVKQIHDSIMNKTIAEKD", "aa_hexdigest": "c5ee275feb6609528749c64ef5df2ee1", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4629.3396999999995, "isoelectric_point": 7.901132392883301 }, "id": "OAIKOHHJFB_1205", "locus": "GCA000209735_01191" }, { "type": "cds", "sequence": "contig_1", "start": 660483, "stop": 661031, "strand": "+", "frame": 3, "gene": null, "product": "glucose-6-phosphate isomerase", "db_xrefs": [ "EC:5.3.1.9", "GO:0004347", "GO:0006096", "SO:0001217", "UniRef:UniRef50_Q4A5S9", "UniRef:UniRef90_A0A3P8LAA1" ], "nt": "ATGTATGGACCTTACAGCATTAACAAACCAAAAATGGAATTATTATTTGCTGGAAATAGCATTAGTAGTGAAAGCTTAGTAGCAGCATTAAAATATGTTGAAAACAAAAAGTTTGCAATTAATGTTATTTCAAAAAGTGGAACAACTTTAGAACCTTCTATTGCTTTTAGAGAATTTAGAAAATTATTAGAAGCAAAAGTTGGAGCAGTAGAAGCTAAGAATTTAATTGTGGCTACTACTGATGCTAAAAAAGGCGTTTTATACTCATTAGCAACTCAAAAAGGATATGAAAAATTAATTATTCCTGATGATGTTGGTGGACGTTTTAGTGTTTTAAGTCCAGTTGGTTTATTCCCATTCATTTGTGCGGGAATTGATATTGAACAAATTCTTAAAGGTGCTCAAGAAGCTAATAAAGATGCAAATGATTTAAGTTTAAAAACAAATGATGCTTATCGTTATGCGGTTGCAAGACATGTTTTAAGTAAAAATTACACCATGGAAATGATGATTTCTTATGAGCCAAAAATGCAATATTTTGCAGAATGA", "aa": "MYGPYSINKPKMELLFAGNSISSESLVAALKYVENKKFAINVISKSGTTLEPSIAFREFRKLLEAKVGAVEAKNLIVATTDAKKGVLYSLATQKGYEKLIIPDDVGGRFSVLSPVGLFPFICAGIDIEQILKGAQEANKDANDLSLKTNDAYRYAVARHVLSKNYTMEMMISYEPKMQYFAE", "aa_hexdigest": "10efbc395c6977cd16e1c7548848d97a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 660207, "stop": 661334, "observations": { "mutation": [ 660327, 660336, 660402, 661029, 661032 ], "stop": [ 660327, 660336, 660402, 661029, 661032 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8LAA1", "query_cov": 1.0, "subject_cov": 0.4262295081967213, "identity": 0.945, "score": 339.0, "evalue": 6.19e-114, "gene_start": 1, "gene_end": 182, "reference_start": 93, "reference_end": 274, "reference_sequence": "MKAVKLNLHNCVDEKLLLSYEDKVKSIHNSIMSKSVVEKDWLGWLELPNKAGSDEVKKMKKIVELWESKNIEAVVVIGIGGSYLGAKSGYEFIYGQYSINKPKMELVFAGNSISSESLVAALKYVEGKKFAINVISKSGTTLEPSIAFREFRKLLEAKVGAVEAKNLIVATTDAKKGVLYSLASQKGYEKLIIPDDVGGRFSVLSPVGLFPFLCAGIDIDQILSGAQAANKDANDLSLKTNDAYRYAVARHILSKNYTMEMMISYEPKMQYFAEWWKQLFAESEGKDGKGLFPTSGIFSTDLHSLGQMIQDGNKVLFETILVLDKPKYDIDFKADKEDLDKLVYLDKNKLHSVNMTAFNATLDAHMNTGKVPNIVMNFAKFDEYTLGYLFQFFERALAISAYLLGVNPFNQPGVEVYKANMFKILKG" }, "paralog": false, "identity": 0.9093333333333333, "score": 667.0, "evalue": 8.46e-246, "description": "Internal stop codon at 660327, 660336, 660402, 661029, 661032. Nonsense mutation around 660327, 660336, 660402, 661029, 661032.", "psc": { "uniref90_id": "UniRef90_A0A3P8LAA1", "product": "glucose-6-phosphate isomerase", "ec_ids": [ "5.3.1.9" ], "uniref50_id": "UniRef50_Q4A5S9", "go_ids": [ "GO:0004347", "GO:0006096" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8LAA1", "EC:5.3.1.9", "UniRef:UniRef50_Q4A5S9", "GO:0004347", "GO:0006096" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5S9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5S9" ], "product": "Glucose-6-phosphate isomerase" } }, "id": "OAIKOHHJFB_1206", "locus": "GCA000209735_01192" }, { "type": "cds", "sequence": "contig_1", "start": 661128, "stop": 661487, "strand": "+", "frame": 3, "gene": null, "product": "glucose-6-phosphate isomerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A971BB97" ], "nt": "ATGATTCAAGATGGAAATAAAGTATTATTTGAAACAATTTTAGTTTTAGATAAACCAAAATACGATATTACTTTTAAAGCTGATAATGAAGATTTAGATAAACTTGTTTATTTAGATAAAAACAAATTGCATAATGTTAACATGACAGCTTTTACAGCAACTTTAGATGCTCACATGAACACAGGTAAAGTTCCAAACATTGTAATGAATTTTGCAAAATTTGATGAATACACATTAGGTTACTTATTCCAATTCTTTGAAAGAGCTTTAGCTATTAGTGCTTATCTTTTAGGTGTTAATCCATTCAACCAACCAGGTGTTGAAGTTTATAAAGCTAATATGTTTAAAATTCTTAAATAA", "aa": "MIQDGNKVLFETILVLDKPKYDITFKADNEDLDKLVYLDKNKLHNVNMTAFTATLDAHMNTGKVPNIVMNFAKFDEYTLGYLFQFFERALAISAYLLGVNPFNQPGVEVYKANMFKILK", "aa_hexdigest": "0ccad641ba841aa1870ecf5ded61c6ad", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A847B363", "query_cov": 0.9831932773109243, "subject_cov": 0.841726618705036, "identity": 0.513, "score": 111.0, "evalue": 2.88e-29, "valid": false, "product": "glucose-6-phosphate isomerase", "uniref50_id": "UniRef50_A0A971BB97" }, "pscc": { "uniref50_id": "UniRef50_A0A971BB97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A971BB97" ], "product": "glucose-6-phosphate isomerase" }, "genes": [], "id": "OAIKOHHJFB_1207", "locus": "GCA000209735_01193" }, { "type": "cds", "sequence": "contig_1", "start": 661670, "stop": 661771, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATATAAAAAAGAAATGTATTATGAAAGATTATTAGATCCAATAATAAGAAAATATTTAAAAGTTTTTGGAGCAATTTGCATTCAAGGACCTAAATGA", "aa": "MTYKKEMYYERLLDPIIRKYLKVFGAICIQGPK", "aa_hexdigest": "4959bb76fbeaae5b28d7f13d39b25b63", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4009.8433000000014, "isoelectric_point": 9.517548179626463 }, "id": "OAIKOHHJFB_1208", "locus": "GCA000209735_01194" }, { "type": "cds", "sequence": "contig_1", "start": 661991, "stop": 662206, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTGGTTCTTCTACTCCTGAACATAAAGGCCTATTTCATAGTGGTATTGGTCGAATTGCTACATTACAATTGAGACCTATGAGCTTATTTGAATCTAAAGATTCTTTAGGACAAATTTCATTAAAAGATTTAATAAATGACAATTTTGAAAATACAACTTATGAAGGTATTGAATTAAATAAAATTATTAATTTAATTATTAGAGGTGGTTGA", "aa": "MTGSSTPEHKGLFHSGIGRIATLQLRPMSLFESKDSLGQISLKDLINDNFENTTYEGIELNKIINLIIRGG", "aa_hexdigest": "e72666246ead2cdd8db9f28b1014b4e9", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7831.846499999996, "isoelectric_point": 6.034504890441894 }, "id": "OAIKOHHJFB_1209", "locus": "GCA000209735_01195" }, { "type": "cds", "sequence": "contig_1", "start": 662483, "stop": 662683, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13635.12" ], "nt": "TTGCAACCATTTAGTTTGAATATTAGATCCAATTTAAGAATTAAACAAAATGAAAAAAGACTTTTTGTCGATCCTTCTTTAGCAGCTGCGTTATTGAATGTTAATAATGAGAGTTTGCAAAATGATTTAAATACTTTGGGTTTTTTATTTGAAAACCTTGTCTTAAGAGATTTGAAAGTTTATGCTCAAAGTAATGAGTGA", "aa": "MQPFSLNIRSNLRIKQNEKRLFVDPSLAAALLNVNNESLQNDLNTLGFLFENLVLRDLKVYAQSNE", "aa_hexdigest": "62a5f12d2a6b060b2dd6002d34aa8ddc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13635.12", "name": "DUF4143", "length": 64, "aa_cov": 0.9696969696969697, "hmm_cov": 0.96875, "evalue": 2.08204056620732e-12, "score": 43.17341995239258, "start": 1, "stop": 64 } ], "seq_stats": { "molecular_weight": 7591.5720999999985, "isoelectric_point": 6.178137016296386 }, "id": "OAIKOHHJFB_1210", "locus": "GCA000209735_01196" }, { "type": "cds", "sequence": "contig_1", "start": 663928, "stop": 664248, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGGGCATTAACCCTTCCTAAAATATTTGGCGACTTGTCGCCCTTTCTAAAATATTATTTTAATTTTCCTATTTTTTTGAAAAACAAATTTCTATTCTTGTTGCTTTCTCAGTTTCTCTTTTGTTTTCCATATAATACTTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCGTACATTTCCAAAACAGATAAATATCCAAAAATTTCTCTTGGACGATTGTTTATTTCATTTTGTACTTTTAAAATTTCTTCTTCAGTAATATCATCAAAATTAGTCTTTTTCTTAAAAAAATCTTCTAACTAA", "aa": "MRALTLPKIFGDLSPFLKYYFNFPIFLKNKFLFLLLSQFLFCFPYNTLSIPVEFQGFSAFNFSYISKTDKYPKISLGRLFISFCTFKISSSVISSKLVFFLKKSSN", "aa_hexdigest": "d4b8debfab38e3e77715dbefa5e800f5", "start_type": "GTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12382.585999999981, "isoelectric_point": 9.913126182556148 }, "id": "OAIKOHHJFB_1211", "locus": "GCA000209735_01197" }, { "type": "cds", "sequence": "contig_1", "start": 664604, "stop": 665425, "strand": "+", "frame": 2, "gene": "mutM", "product": "bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase", "db_xrefs": [ "EC:3.2.2.23", "EC:4.2.99.18", "GO:0003676", "GO:0003684", "GO:0003906", "GO:0006281", "GO:0006284", "GO:0008270", "GO:0008534", "GO:0016799", "GO:0019104", "KEGG:K10563", "RefSeq:WP_013526847.1", "SO:0001217", "UniParc:UPI00000B1D7B", "UniRef:UniRef100_C4XFD9", "UniRef:UniRef50_A0A974WKQ3", "UniRef:UniRef90_C4XFD9" ], "nt": "ATGCCAGAATTACCAGAAGTTAGAAGTGTGGTCAAAGATTTAAGACCTAAAGTTGTTAATAGAAAAATAGTTAAAATTGATATCTTACATCCTAAATTAATTAAAGAGGTAAGTGTTGAAGAATTCAAGAATTTTTTAATTAATGAAACATTTTTAGATGTCAATAATTTAGCTAAACATATTATTTTTTCTTTAACAAATAATAAATATTTATTAAGCCACTTGAGAATGAGTGGAAAGTATTTTACACATTATAAATATCGTCCAGCAACAAAACATGATTACTTAATTTTTCATCTTGATGATAATAGTTGTATTTATTATAATGACTCACGTCAATTTGGCACTTTTCATATAAAAACTAAGGGCACTTTATATACAACTAAACCTTTAGATAAAGTTGCAAAAATTCCTTCAGAAACAAATATTAAAGAGTTATTTAATAAAATTAAAAATAAGAATATACCTATAAAACAGCTTCTTTTGGATCAAAGCTTTGTTTCAGGTATTGGAAATATTTATGCTAATGAAACTTTATTTGCCACTCAAATTAATCCTTTGACGCCTTCAAAAAATATAACTTTTGAACAGTTAGAAAAAATTATTAAAGCAGCTGCAAAAATTATGGATCAAGCTACTGAATTAGGTGGTTCAAGCATTGACACATATACTTCAGTTGATGGAGTTAAAGGTCAATTTCAAGATTTTTTACAAGTTCATGGACACTTTAATGATGTATGTAAAAGATGTAAAAAAGCTAAAATTAATAAAATTTTTATTAACAAAAGAGGGACTTATTATTGTCCAAATTATCAAAAATAA", "aa": "MPELPEVRSVVKDLRPKVVNRKIVKIDILHPKLIKEVSVEEFKNFLINETFLDVNNLAKHIIFSLTNNKYLLSHLRMSGKYFTHYKYRPATKHDYLIFHLDDNSCIYYNDSRQFGTFHIKTKGTLYTTKPLDKVAKIPSETNIKELFNKIKNKNIPIKQLLLDQSFVSGIGNIYANETLFATQINPLTPSKNITFEQLEKIIKAAAKIMDQATELGGSSIDTYTSVDGVKGQFQDFLQVHGHFNDVCKRCKKAKINKIFINKRGTYYCPNYQK", "aa_hexdigest": "d8905f5119a432459c837c4c7f468128", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000B1D7B", "ncbi_nrp_id": "WP_013526847.1", "uniref100_id": "UniRef100_C4XFD9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000B1D7B", "RefSeq:WP_013526847.1", "UniRef:UniRef100_C4XFD9" ] }, "ips": { "uniref100_id": "UniRef100_C4XFD9", "uniref90_id": "UniRef90_C4XFD9" }, "psc": { "uniref90_id": "UniRef90_C4XFD9", "gene": "mutM", "product": "bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase", "ec_ids": [ "3.2.2.23", "4.2.99.18" ], "uniref50_id": "UniRef50_A0A974WKQ3", "kegg_orthology_id": "K10563", "go_ids": [ "GO:0003676", "GO:0003684", "GO:0003906", "GO:0006281", "GO:0006284", "GO:0008270", "GO:0008534", "GO:0016799", "GO:0019104" ] }, "pscc": { "uniref50_id": "UniRef50_A0A974WKQ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A974WKQ3" ], "product": "Bifunctional DNA-formamidopyrimidine glycosylase/DNA-(Apurinic or apyrimidinic site) lyase" }, "genes": [ "mutM" ], "id": "OAIKOHHJFB_1212", "locus": "GCA000209735_01198" }, { "type": "cds", "sequence": "contig_1", "start": 665458, "stop": 665766, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13635.12" ], "nt": "TTGTGTTCTAGAGATTTAAAAATATATTCATCAAGTTTAAATGGAGAATTATCATATTATCACGATAGATATGGTCTTGAAATTGATTTTGTTTTGCACTTAAATGATGGCAGATATGCATTGATAGAATGTAAATTAGGTGGAAATCAAATTGAAAAAGCGAAAAATAATTTATTAAAAGTAAAACAGCTGATAAAAGAAAATGGACAAAGAGAACCGGATCTTATGATTATTTTAACAGCAGAAGATAGAGCATATGAAATAACAGATGGAATACTTATTATTCCAATTAGTCTACTAAAAGATTAA", "aa": "MCSRDLKIYSSSLNGELSYYHDRYGLEIDFVLHLNDGRYALIECKLGGNQIEKAKNNLLKVKQLIKENGQREPDLMIILTAEDRAYEITDGILIIPISLLKD", "aa_hexdigest": "50c3ca6f07f0e61a10b35c2d9dfeaa70", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_G6AUJ2", "query_cov": 0.9019607843137255, "subject_cov": 0.968421052631579, "identity": 0.522, "score": 95.1, "evalue": 1.46e-23, "valid": false, "product": "DUF4143 domain-containing protein", "uniref50_id": "UniRef50_G6AUJ2" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13635.12", "name": "DUF4143", "length": 36, "aa_cov": 0.3431372549019608, "hmm_cov": 1.0, "evalue": 1.4188833555454285e-08, "score": 30.7131404876709, "start": 12, "stop": 46 } ], "seq_stats": { "molecular_weight": 11685.353499999987, "isoelectric_point": 5.352891731262207 }, "id": "OAIKOHHJFB_1213", "locus": "GCA000209735_01199" }, { "type": "cds", "sequence": "contig_1", "start": 665911, "stop": 666438, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAAAAAGGGGTTATTTATTTTAACAGGCTCAAGTACACACAATAAATTAAATACTATGCATACTGGTACTGGTAGAATACATACAATGAAAATGTATCCAATGAGTTTATATGAAACTGGAGAATCTAGTGGAAAAATATCTTTACAAGATTTATTTGATAAAAAAATTAAAGTAAATGGTTTAAAATCAAATTTATCTTTTGAAGATTTAGCAAAAGCAATTTGCAGAGGAGGATGGCCAAGTACTCTAAATTTAAAAAAATATGAAAATCAAATCGAAATTCCTAAAGATTATGTCAATCAAATTTGCAATGTAGATATAAGTAAAATTGATAACATAAAAAGAAGCCCTGAAATTACTAGAAGAATATTGAAAAGTTATGCAAGAAATGTTGCTACTTTAGCTACTTCAAAAACTTTAATAAAAGATTGTAGTTTTAAAGATGAAGGTTCAAAAACAACTTTTTATGATTACACTACAAAACTTGAAGATTTAAATGTTATTGATAATATAACTGCATGA", "aa": "MGKKGLFILTGSSTHNKLNTMHTGTGRIHTMKMYPMSLYETGESSGKISLQDLFDKKIKVNGLKSNLSFEDLAKAICRGGWPSTLNLKKYENQIEIPKDYVNQICNVDISKIDNIKRSPEITRRILKSYARNVATLATSKTLIKDCSFKDEGSKTTFYDYTTKLEDLNVIDNITA", "aa_hexdigest": "a60c6c17d3e853e2d6fa181ffec30ae7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19724.474400000006, "isoelectric_point": 9.331621360778808 }, "id": "OAIKOHHJFB_1214", "locus": "GCA000209735_01200" }, { "type": "cds", "sequence": "contig_1", "start": 666490, "stop": 666723, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATATAAAAGAAGAATAATAGAAAAACAAATAAAAAAAAATCTTGAAATTTTTGGTGCTATAAACATAGAAGGAATTAAACAATGTGGTAAAACAACTACTGCACAAAAATTTTCAAAAACATTAATTAAATTACAAGATCCAGACAATTTAAAAACTTACAAAGAAACAATTGAATATAAACCTTCATTACTTTTAGAAGGAGAAAAACCATTATTAATAGATGAATGA", "aa": "MKYKRRIIEKQIKKNLEIFGAINIEGIKQCGKTTTAQKFSKTLIKLQDPDNLKTYKETIEYKPSLLLEGEKPLLIDE", "aa_hexdigest": "ce4ef8650e664774e20486f27b1139a7", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8968.482799999996, "isoelectric_point": 9.380681800842286 }, "id": "OAIKOHHJFB_1215", "locus": "GCA000209735_01201" }, { "type": "cds", "sequence": "contig_1", "start": 667426, "stop": 667746, "strand": "-", "frame": 2, "gene": null, "product": "Amino acid permease/ SLC12A domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE2", "UniRef:UniRef90_C4XFE2" ], "nt": "ATGATTACAGTTATAATAAATTATTCTTTAATTGCTATTTTTGCAATTTTACATAATAGTGGAGTTGTTGAACAAATAATTAAAAATACAATTGATCAAAGAACAAATCAACATTTAGTGCAAAGTTTAAGCATTATTTTTAGTCTATTCTTATTTATTGCTGTTGTTGGAGTATTAAATGATTTTACCAATTCATATGCACATAATATTAATAATGCTATTAATTTAAAAATAATAATTAATACTACTAAAATAAGAAACAAAATGAATCTTAAAAGAATGATGATTTTAATTATAAGTCTTATTTATAGTGCTATTTGA", "aa": "MITVIINYSLIAIFAILHNSGVVEQIIKNTIDQRTNQHLVQSLSIIFSLFLFIAVVGVLNDFTNSYAHNINNAINLKIIINTTKIRNKMNLKRMMILIISLIYSAI", "aa_hexdigest": "298fdedea63d1b0b8f16822e0146dae8", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 667369, "stop": 667983, "observations": { "mutation": [ 667404, 667428, 667872 ], "stop": [ 667404, 667428, 667872 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE2", "query_cov": 1.0, "subject_cov": 0.5196078431372549, "identity": 1.0, "score": 187.0, "evalue": 2.13e-58, "gene_start": 1, "gene_end": 106, "reference_start": 80, "reference_end": 185, "reference_sequence": "MINDLNFSGVNLISLYNALSFNKTDQLKNTKTNGISNWATQLGNKKFCYFISFNYTFLSVGSTANNLKKDNLPTVAIVGMITVIINYSLIAIFAILHNSGVVEQIIKNTIDQRTNQHLVQSLSIIFSLFLFIAVVGVLNDFTNSYAHNINNAINLKIIINTTKIRNKMNLKRMMILIISLIYSAIWLIISICIWVLGGDNILDG" }, "paralog": false, "identity": 0.9803921568627451, "score": 382.0, "evalue": 5.99e-138, "description": "Internal stop codon at 667404, 667428, 667872. Nonsense mutation around 667404, 667428, 667872.", "psc": { "uniref90_id": "UniRef90_C4XFE2", "product": "Amino acid permease/ SLC12A domain-containing protein", "uniref50_id": "UniRef50_C4XFE2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE2", "UniRef:UniRef50_C4XFE2" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE2" ], "product": "Amino acid permease/ SLC12A domain-containing protein" } }, "id": "OAIKOHHJFB_1216", "locus": "GCA000209735_01202" }, { "type": "cds", "sequence": "contig_1", "start": 668480, "stop": 668716, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCCCACAATCTTTAGCTTTAGGTGTAATCATTGGACATATTTTTCCAGTTTACTTTAAATTTAAAGGTGGTAAAGGTGTAGCTTGTACAATTGGTTTAATTGCCACAATTAATATTGTCTTTTTATTAATGGCTTCTATTATCTTCTTTATAGTTGTCTTAACTTCTAAATATGTATCTTTAGCTTCAATATTAACAGCGGCAATATTAGTGCCCTTTGCTTTTATTCCTTGA", "aa": "MSPQSLALGVIIGHIFPVYFKFKGGKGVACTIGLIATINIVFLLMASIIFFIVVLTSKYVSLASILTAAILVPFAFIP", "aa_hexdigest": "8d66a0f3ff6af79c81325c8b74145ed0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8288.133699999991, "isoelectric_point": 9.748087120056148 }, "id": "OAIKOHHJFB_1217", "locus": "GCA000209735_01203" }, { "type": "cds", "sequence": "contig_1", "start": 668753, "stop": 668956, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTATGAATATCACAATTGCTTTGTAACAAGTTTTATCTATTTAATAGCTGCAATATTAATTATTGCAATGCATCATAGTAATATTACTCGTTTAATTAATCATACAGAAAGTAAATTAGGCCAAAAAAAAAGCGAAGATAATTCAGATGATACTGAAGTTGTTTTAATTAGTGAAAAAGAAGAACATGAATCTACTAAATAA", "aa": "MYEYHNCFVTSFIYLIAAILIIAMHHSNITRLINHTESKLGQKKSEDNSDDTEVVLISEKEEHESTK", "aa_hexdigest": "dd1c6d49167307d3b3f504491373fbb0", "start_type": "GTG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7766.662299999998, "isoelectric_point": 5.370454978942871 }, "id": "OAIKOHHJFB_1218", "locus": "GCA000209735_01204" }, { "type": "ncRNA-region", "class": [ "riboswitch", "SO:0000035" ], "sequence": "contig_1", "start": 668990, "stop": 669091, "strand": "+", "label": "TPP", "product": "TPP riboswitch aptamer (THI element)", "score": 54.3, "evalue": 8e-09, "db_xrefs": [ "RFAM:RF00059", "SO:0000035" ], "nt": "TGAAATTGACAGAGGTATTTGCTTATATAACAAGCAGATTGAGAAAAACTCTTAGAGCTGATCAGGTTAATACCTGCGGAGCAAGTTCAATTAATTTGAATT", "id": "OAIKOHHJFB_45" }, { "type": "cds", "sequence": "contig_1", "start": 669143, "stop": 669448, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATATATAAATTTATTGTAGCAAGTTCAAGTATTTTTGCTTTAGGAAGTATTAGTGCTGGTTGCCAATATCAACCTAATATTGAAGAAATAAAAATTAAAATGGAAGGTATTAAATATGCAGATAAAGAAAAGTTAGATGCATATACAAAAGTATTTAATCAAGTTTTTAATATGAAAATGAAAGAAGCAAATTTAAATCAAAAAGTTGTTTCGTTTCATGATTCAGGAAATGGTGAAAGCGCAACACTTAAAGAAGTTAATTTAAACAAATTTCAAGTGGCTTTTCCCGGAGCTTGA", "aa": "MKKIYKFIVASSSIFALGSISAGCQYQPNIEEIKIKMEGIKYADKEKLDAYTKVFNQVFNMKMKEANLNQKVVSFHDSGNGESATLKEVNLNKFQVAFPGA", "aa_hexdigest": "438388db6a770fa377e83dcdf6951637", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11302.961399999991, "isoelectric_point": 9.187276649475098 }, "id": "OAIKOHHJFB_1219", "locus": "GCA000209735_01205" }, { "type": "cds", "sequence": "contig_1", "start": 669467, "stop": 669652, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAATAAAAATAAAAATGTTGTAGCTTTTATGCAAACAAAAACTAGAGCTTTTAAAACAAAAAGTAGTTTAAATTACAATGATCAAAGTCTTTATGATCCAAGTAAAAGTAATAACTTCGCAACTTTACAAGCTCAATATGAAAATGAATTATTCAATAGTGAAGGTGGCTATGTAAATTGA", "aa": "MNNKNKNVVAFMQTKTRAFKTKSSLNYNDQSLYDPSKSNNFATLQAQYENELFNSEGGYVN", "aa_hexdigest": "817667c37054e92ce57c53d97b033df4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7004.609200000002, "isoelectric_point": 9.049507713317869 }, "id": "OAIKOHHJFB_1220", "locus": "GCA000209735_01206" }, { "type": "cds", "sequence": "contig_1", "start": 669974, "stop": 670243, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGCAAGGATAATTCTTCAAATTATCATATAGCTTTTGATAATGAGAATTCTTTTGCTTATAGTAATCATGGAAATAAAGCATTAGAAAATAGAGATTTTTATGTTAAAGATAAAAATAAAACTTATGAAATGTTAACTTTAACTGATCCTATTCCTTATAATGTGGCTATAGCAAATAATTCACTAAGTAAAAATGTATTAGAATTATTAGCTCAAACTTATATTGAATTAGCAAATAAAAATAATAATTATGTTGGAAATTTATGA", "aa": "MGKDNSSNYHIAFDNENSFAYSNHGNKALENRDFYVKDKNKTYEMLTLTDPIPYNVAIANNSLSKNVLELLAQTYIELANKNNNYVGNL", "aa_hexdigest": "764a55c8c98340f759798a3f323c0fc6", "start_type": "TTG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10138.073699999995, "isoelectric_point": 5.528865242004394 }, "id": "OAIKOHHJFB_1221", "locus": "GCA000209735_01207" }, { "type": "cds", "sequence": "contig_1", "start": 670458, "stop": 670661, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAAAGCTATTTTAGAACCAAAATTAATAGCTAAAGGGCAAATATTAATTAATGATCAAGATATCAATAAAATTGATCAAAAGACCTATAAAACAGTTAAAAAAGATATTTCTTTTTTATTTCAAGAGCCTTCTTTAATTGAGACAGATGATGTCATTGAAAATATTAAAAGAAAGTCAAATAATATCTATAATAATTGA", "aa": "MIKAILEPKLIAKGQILINDQDINKIDQKTYKTVKKDISFLFQEPSLIETDDVIENIKRKSNNIYNN", "aa_hexdigest": "f7661284abff81a9b481ca7b3c3ea91a", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7805.951699999998, "isoelectric_point": 7.943101310729982 }, "id": "OAIKOHHJFB_1222", "locus": "GCA000209735_01208" }, { "type": "cds", "sequence": "contig_1", "start": 670791, "stop": 671060, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAACTAGCCTCAATCTTTTTTGAAAATAAAAATATTATTTTGGCTGATGAACCAACAAGTAATTTAGATTTTATTAATACTAAAAAAATATTAAAATATTTGCAAAAATATGTTCATGATAATAATGCATTATTATTATGTAACATTCATGATGTTACTAATTCGCTAGAATATATGGATTATATTTTATTATTAAAAAATAAGAGACTTGTTTTTATTAAACAAGCTAATAATATAAGCGAAAAAGAATTTCAAGAACAATATTAA", "aa": "MELASIFFENKNIILADEPTSNLDFINTKKILKYLQKYVHDNNALLLCNIHDVTNSLEYMDYILLLKNKRLVFIKQANNISEKEFQEQY", "aa_hexdigest": "76cb33609cef8b1a96b426de9eb5cd1f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10586.088499999993, "isoelectric_point": 5.592013359069824 }, "id": "OAIKOHHJFB_1223", "locus": "GCA000209735_01209" }, { "type": "cds", "sequence": "contig_1", "start": 671197, "stop": 671715, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAGCGAAAGTTATTCTATTTTCAAAGAATTACTAATTACTATCAAAATTACTATTGCCAGCATTTTCTTAGCTTTTATCTTTTCAATTTTTGCTTCTTTTTTTGCAAACGATAAAACAAATAATAAATTTGTGAAAATAATAAGTAAAATGCTTTTAGCATTCTTTAGAACTATACCAATAACCTTATTTTTCTATTTATCAATTAATCTTTATTATTCAAGAGAAGTCATTTTAATTTTAGCTTTATCTTTTACTACAATGCGTTCAATGAGTAAATTTTATACTGAATCAATTAATAATATTGATCAAAAATTAATTGGCAATTATTTATTATTAAATAAAAATAAAATAACTCTATATTTTGAATTTATTTTACCTAGAGTAATTAAAGATTTTTATAGTTATGCAGCTTTTAGATTCGAATTAATCTTTAGAAACATTATTAATTATGGAACTTTTGCAGCTGTTGGTTTAGGCGCTAGATTAAATTATTACCAAAACAAAAATGAATGA", "aa": "MKSESYSIFKELLITIKITIASIFLAFIFSIFASFFANDKTNNKFVKIISKMLLAFFRTIPITLFFYLSINLYYSREVILILALSFTTMRSMSKFYTESINNIDQKLIGNYLLLNKNKITLYFEFILPRVIKDFYSYAAFRFELIFRNIINYGTFAAVGLGARLNYYQNKNE", "aa_hexdigest": "2056a0524c234575a59fe4e34dc7d20a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20263.694399999993, "isoelectric_point": 9.74731349945068 }, "id": "OAIKOHHJFB_1224", "locus": "GCA000209735_01210" }, { "type": "cds", "sequence": "contig_1", "start": 672464, "stop": 672685, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTACTTCATAAAAAACTTAAAGATAAAATTCATAAATTATTTGCGGGATATGAAGAGTTTATATTATTAAAAGTAAATAATCTTTGTAGATCATTTTTTAATTGTTTTACTGAATTATTGTCCATAATTTTTCAATCAAAAGATAATAATGAATATAAACAAATTATTAGAATAAGCAAAATAAAAGTTACTTTAACTCATCTTTTGAGTTTTTTATAA", "aa": "MVLHKKLKDKIHKLFAGYEEFILLKVNNLCRSFFNCFTELLSIIFQSKDNNEYKQIIRISKIKVTLTHLLSFL", "aa_hexdigest": "a96987788128ee145e4ed8e57011b5a6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8718.346999999992, "isoelectric_point": 9.596844291687013 }, "id": "OAIKOHHJFB_1225", "locus": "GCA000209735_01211" }, { "type": "cds", "sequence": "contig_1", "start": 673061, "stop": 673192, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAGCACAAAAAAAATTGTAGCTTTAACAATGGATATTTCTGCCGGGATTGTTGGTGCAGTATCAGCTGGTACTTTAATTGGTTATGCAGTTGACCATTCAAACTATAAAAAAGAAAAATTGTGA", "aa": "MKNSTKKIVALTMDISAGIVGAVSAGTLIGYAVDHSNYKKEKL", "aa_hexdigest": "822f02c64f2d226d13eb065cc39a95f4", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4524.263599999999, "isoelectric_point": 9.523801612854005 }, "id": "OAIKOHHJFB_1226", "locus": "GCA000209735_01212" }, { "type": "cds", "sequence": "contig_1", "start": 673322, "stop": 673786, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7", "UniRef:UniRef90_C4XFE7" ], "nt": "TTGTGTCAAACTCAAATTGACTCAAATGAAAAACTTATTAAAGATTTACAAGGAATTATTGATCAACACTCTTCATATCATGTTGGCCGCCGCTTTGGTAACAAAAGTTATATGAGAGCTGTAAAACATAGTAATGAAAAGAAAATAATTCAAAATCTTTTACCAGAAATGGTGGAAAGAACAAAACAAGTTATTAATCAAAGCGAATATGAAAATATCAAAAAATCAATCCAAGATTTTGTTGTTCGTAGTAATGATTTATTAACTTTAAAAACTGCAGATGAAAAATTTGTTGATGCTAATAGTAAAAGTGAATTAAATTCTTTAGAACTTAAAATTAAAGATTATCAAAACACTTTAAGTAAAATCGAAGAAGAAGCACTTTCAAGTAACACAAAACCTAACATTTCAACTGAAATGATTAATAAGGCAGCAGATTTTATTAAATCTGATTTTCATGTTTGA", "aa": "MCQTQIDSNEKLIKDLQGIIDQHSSYHVGRRFGNKSYMRAVKHSNEKKIIQNLLPEMVERTKQVINQSEYENIKKSIQDFVVRSNDLLTLKTADEKFVDANSKSELNSLELKIKDYQNTLSKIEEEALSSNTKPNISTEMINKAADFIKSDFHV", "aa_hexdigest": "ccb8803a02cc51d36329f58c8d23c026", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 673061, "stop": 673828, "observations": { "mutation": [ 673190, 673268, 673292, 673307, 673784 ], "stop": [ 673190, 673268, 673292, 673307, 673784 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE7", "query_cov": 1.0, "subject_cov": 0.6039215686274509, "identity": 0.9940000000000001, "score": 286.0, "evalue": 3.25e-96, "gene_start": 1, "gene_end": 154, "reference_start": 88, "reference_end": 241, "reference_sequence": "MKNSTKKIVALTMDISAGIVGAVSAGTLIGYAVDHSNYKKEKLWIENNIKYLKESNQKLKEKDNKLKEIWKKYENQEWLSSLWNELSLCQTQIDSNEKLIKDLQGIIDQHSSYHVGRRFGNKSYMRAVKHSNEKKIIQNLLPEMVERTKQVINQSEYENIKKSIQDFVVRSNDLLTLKTADEKFVDANSKSELNSLELKIKDYQNTLSKIEEEALSSNTKPNISTEMINKAADFIKSDFHVWNDKIIKKYVKKTA" }, "paralog": false, "identity": 0.9490196078431372, "score": 457.0, "evalue": 6.95e-166, "description": "Internal stop codon at 673190, 673268, 673292, 673307, 673784. Nonsense mutation around 673190, 673268, 673292, 673307, 673784.", "psc": { "uniref90_id": "UniRef90_C4XFE7", "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE7", "UniRef:UniRef50_C4XFE7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17764.905099999993, "isoelectric_point": 6.913292503356933 }, "id": "OAIKOHHJFB_1227", "locus": "GCA000209735_01213" }, { "type": "cds", "sequence": "contig_1", "start": 673839, "stop": 674471, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8", "UniRef:UniRef90_C4XFE8" ], "nt": "ATGTCAAACATAGATAAAATAGAAGAATTATCAGCAAAAATAGAAGATGCTAATACTATTGCAAGTAATAATTCTCCAATTTTAAATCCTATTAATGCTGTTAAAAAGATTTTATTGTCAAGATTTGACTCTAATAATTATGACGTTATATTAGAAAAAAACGGTGTTTCAGTAAATAGAAGTGTTATTATAGAAGATATTATTAAAGCATGTAAAACATTAGAACAATCATATGGAAATACGAAGGAATATATAAATCGTTATGTTAATACTTTTGTCAAAACAATTGAAAATACAAAATTATTTAACAAATCTCAAATTCTAGACTTAAGCAACAAATCATTAACACCTATTATTAATGGTCTTAATGAATTTAACTCATTTGTTGAAAGTTCAAATCAAATTTCTTTTGAAAAATTAGATGAATTTATTAACAAAATTCAAGCTATTCTAAATGTTGAAAATGATTTAACAACTTCTATTCAGTCTGTTATTAAAGAATTTTTAAATGCCTATAATTCAAATGCTGATTCAATTGAAACAACAGTTAAATTAGCTAAACTAAATAACTCTAAACAAATTATTGATGAATTTGTACAAAAATCTAAAATTGAATTAAGTTCTCTCAAATGA", "aa": "MSNIDKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILEKNGVSVNRSVIIEDIIKACKTLEQSYGNTKEYINRYVNTFVKTIENTKLFNKSQILDLSNKSLTPIINGLNEFNSFVESSNQISFEKLDEFINKIQAILNVENDLTTSIQSVIKEFLNAYNSNADSIETTVKLAKLNNSKQIIDEFVQKSKIELSSLK", "aa_hexdigest": "eeab87916b5d962c26bbd893192ae7b5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 673839, "stop": 674669, "observations": { "mutation": [ 674469 ], "stop": [ 674469 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE8", "query_cov": 1.0, "subject_cov": 0.7608695652173914, "identity": 1.0, "score": 389.0, "evalue": 1.47e-135, "gene_start": 1, "gene_end": 210, "reference_start": 1, "reference_end": 210, "reference_sequence": "MSNIDKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILEKNGVSVNRSVIIEDIIKACKTLEQSYGNTKEYINRYVNTFVKTIENTKLFNKSQILDLSNKSLTPIINGLNEFNSFVESSNQISFEKLDEFINKIQAILNVENDLTTSIQSVIKEFLNAYNSNADSIETTVKLAKLNNSKQIIDEFVQKSKIELSSLKWDNYIEGRNKLINEYVNSKNELDSIKNGFKKAFVNIKSRLNEFNLTLEANKTTLNEFYLFETSYKS" }, "paralog": false, "identity": 0.9963768115942029, "score": 527.0, "evalue": 2.53e-192, "description": "Internal stop codon at 674469. Nonsense mutation around 674469.", "psc": { "uniref90_id": "UniRef90_C4XFE8", "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE8", "UniRef:UniRef50_C4XFE8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 23689.56930000002, "isoelectric_point": 5.2369401931762685 }, "id": "OAIKOHHJFB_1228", "locus": "GCA000209735_01214" }, { "type": "cds", "sequence": "contig_1", "start": 675199, "stop": 675366, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGCAAAATTAGCAGCAATAGCAAATGTGGATTCTAAAGTATATGAATATAAAAACAAAATTTTTGATGCTGCAATTGAAGCTTATAAAAATGTTAATTCTACAGAAAACTTTAGTGCTGTTCAAGCTGATAAAGACAACAAAGTTAACTTTGTTAAAAAATGA", "aa": "MDAKLAAIANVDSKVYEYKNKIFDAAIEAYKNVNSTENFSAVQADKDNKVNFVKK", "aa_hexdigest": "ffe7870c92ac45336f99773804f27cea", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6172.882700000004, "isoelectric_point": 7.942263221740723 }, "id": "OAIKOHHJFB_1229", "locus": "GCA000209735_01215" }, { "type": "cds", "sequence": "contig_1", "start": 675478, "stop": 675696, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCAATTAAAAATGATCAAATTTCTAAATTAGATAGTGAAGGTTTAAAAGCTTTGCAACAACTTGATGGTGGTGTTGATTTTACAAATAATGATTTAATTAAAAATTATTTAGAAAATGATTATTTATCAGCTGTTAAAGATTTAAGAGAATATTTCTTAAACAAACTAATTTACTTATTACATGAAAAATTTAATTATGGTGATGTATACTGA", "aa": "MNAIKNDQISKLDSEGLKALQQLDGGVDFTNNDLIKNYLENDYLSAVKDLREYFLNKLIYLLHEKFNYGDVY", "aa_hexdigest": "1d4319ff09a22acc5365a504b9799701", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8406.355899999999, "isoelectric_point": 4.6834421157836905 }, "id": "OAIKOHHJFB_1230", "locus": "GCA000209735_01216" }, { "type": "cds", "sequence": "contig_1", "start": 675678, "stop": 675953, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTCACTAGTAGTATTTCCTTTATCTACTCCATATCGGTGTCATGAATATTCAGCTCTAACTTTATCACCTTTAGAATTTCCTCAGCCTCCATTTGTATTACCTTTTGAGTATCCTCCAGTATCTAAATCTGAAATACGATCATATAGATGTTTTTTGCCTAAAACTTTAGGTTTAGCTCAAATTTTTGATTTTTCATCTCAATTTGCATTATTTTGTGAGACAGATGTTGGTCTATACAATTTTGTTGGAGATCAGTATACATCACCATAA", "aa": "MISLVVFPLSTPYRCHEYSALTLSPLEFPQPPFVLPFEYPPVSKSEIRSYRCFLPKTLGLAQIFDFSSQFALFCETDVGLYNFVGDQYTSP", "aa_hexdigest": "f6ce9c8dd16ef992161b08df3311427a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10396.849499999993, "isoelectric_point": 4.794903373718263 }, "id": "OAIKOHHJFB_1231", "locus": "GCA000209735_01217" }, { "type": "cds", "sequence": "contig_1", "start": 676135, "stop": 676341, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCCAAATTAATAGAATATGAGAAAACAGTAACTGAAAAAATGCTTTCAAATATCAAAAAAATATCAGATTCTAAAATAATGTTTAAAAATGATTTTAAAGACAATTTTGTTAATTTAAACGATGCTCTTTACAAAATAAAATATGCTTTTGATTTCATTAAATATAACAATCCAACAAGCAATGGATATTATGAAGGAATATGA", "aa": "MAKLIEYEKTVTEKMLSNIKKISDSKIMFKNDFKDNFVNLNDALYKIKYAFDFIKYNNPTSNGYYEGI", "aa_hexdigest": "84a9c29ce6fbec573d274b5d361b5d54", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8060.188600000002, "isoelectric_point": 8.803109550476076 }, "id": "OAIKOHHJFB_1232", "locus": "GCA000209735_01218" }, { "type": "cds", "sequence": "contig_1", "start": 677291, "stop": 677458, "strand": "-", "frame": 1, "gene": null, "product": "Mutator family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1I1BC38" ], "nt": "ATGTTTGATTATTCTCCTGAAATAAGAACGCTTATTTATACTACTAATCTAATCGAAAATTTAAATAGTGTATTTAGAAAATATACAAAAACAAAAAGGGTTTTCCCATCTGATGATAGTCTTTTAAAAATTATTTTTCTAGCATCTCAACAAATCATAAAAAAATGA", "aa": "MFDYSPEIRTLIYTTNLIENLNSVFRKYTKTKRVFPSDDSLLKIIFLASQQIIKK", "aa_hexdigest": "67e4356b6ed447bfc4d9282f75679d63", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1I1BC38", "query_cov": 0.9818181818181818, "subject_cov": 0.8852459016393442, "identity": 0.574, "score": 68.6, "evalue": 2.97e-14, "valid": false, "product": "Mutator family transposase", "uniref50_id": "UniRef50_A0A1I1BC38" }, "pscc": { "uniref50_id": "UniRef50_A0A1I1BC38", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1I1BC38" ], "product": "Mutator family transposase" }, "genes": [], "id": "OAIKOHHJFB_1233", "locus": "GCA000209735_01219" }, { "type": "cds", "sequence": "contig_1", "start": 677519, "stop": 677785, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10551.15" ], "nt": "ATGTCTGTCCTAAATGCTCTTAAAAATAGAGGAATTAGTAAAATTAATATTATTTGTTCAGATAATTTAAAAGGTATACAACAATCTATAGAAGCTGTTTTTCCTGATAGTAAGCAACAACGTTGCATTGTACATATGATTAGAAATTCTGTTAAATATGTTAACTACAAAGATATGAAAGAATTTTGCAAAGATTTAAAATCTGTATATCAATCTAATGATGTAAAAAATGCAATGGATAATTTGGATATTTTTGAAGATAAATGA", "aa": "MSVLNALKNRGISKINIICSDNLKGIQQSIEAVFPDSKQQRCIVHMIRNSVKYVNYKDMKEFCKDLKSVYQSNDVKNAMDNLDIFEDK", "aa_hexdigest": "21cc45ab9b26762f583e32f8a16b7771", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10551.15", "name": "MULE", "length": 41, "aa_cov": 0.4659090909090909, "hmm_cov": 1.0, "evalue": 4.780882784748458e-08, "score": 29.49219512939453, "start": 9, "stop": 49 } ], "seq_stats": { "molecular_weight": 10173.664599999995, "isoelectric_point": 8.806848716735843 }, "id": "OAIKOHHJFB_1234", "locus": "GCA000209735_01220" }, { "type": "cds", "sequence": "contig_1", "start": 677966, "stop": 678103, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATATTTGTTTTATATTCTAGAGGAATGCCAACAAGAGATGTAAGTGATTTTATGTTTAGCAAATATGGCGTTAATTATTCTCCAGCACAAATAAGCCAATTAACTAATGAAATTGTCGAAGATGTAAGATTATGA", "aa": "MIFVLYSRGMPTRDVSDFMFSKYGVNYSPAQISQLTNEIVEDVRL", "aa_hexdigest": "54078c20f8f59997e722d9839537b3cb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5218.9333, "isoelectric_point": 4.8610639572143555 }, "id": "OAIKOHHJFB_1235", "locus": "GCA000209735_01221" }, { "type": "gap", "sequence": "contig_1", "start": 678458, "stop": 678458, "strand": ".", "length": 1, "id": "OAIKOHHJFB_1807" }, { "type": "gap", "sequence": "contig_1", "start": 678560, "stop": 678659, "strand": ".", "length": 100, "id": "OAIKOHHJFB_1808" }, { "type": "cds", "sequence": "contig_1", "start": 679236, "stop": 679442, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCCAAATTAATAGAATATGAGAAAACAGTAACTGAAAAAATGCTTTCAAATATCAAAAAAATATCAGATTCTAAAATAATGTTTAAAAATGATTTTCAAGGCAATTTTGTTAATTTAAATGATGCTCTTTACAAAATAAAATATGCTTTTGATTTTATTAAATACAACAATCCAACAAGCAATGGATATTATGAAGGAATATGA", "aa": "MAKLIEYEKTVTEKMLSNIKKISDSKIMFKNDFQGNFVNLNDALYKIKYAFDFIKYNNPTSNGYYEGI", "aa_hexdigest": "512f6d302678aef6e46dcbb1e9f8c22a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8002.109400000004, "isoelectric_point": 8.830702018737792 }, "id": "OAIKOHHJFB_1236", "locus": "GCA000209735_01222" }, { "type": "gap", "sequence": "contig_1", "start": 679816, "stop": 679915, "strand": ".", "length": 100, "id": "OAIKOHHJFB_1809" }, { "type": "cds", "sequence": "contig_1", "start": 680321, "stop": 680716, "strand": "-", "frame": 1, "gene": null, "product": "Mutator family transposase", "db_xrefs": [ "RefSeq:WP_258408935.1", "SO:0001217", "UniParc:UPI0002DAEC8E", "UniRef:UniRef100_C4XFF2", "UniRef:UniRef50_C4XFF2", "UniRef:UniRef90_C4XFF2" ], "nt": "ATGAAAAGAAAAACAATAAATCCTGTTATTGATGAAATAACAGAAAAAATTTTAGAAAATTATAATCCAGTTACTTCTGGTGATCTTAGTATGGCTATGAAAGAAGTATTTCAAAATACAATCCAAAAAATGATGAACAAAGAATTTGATAATTTTATGGGTTATGAAAAAAATGATAATAAAGTTCAAAAAAATTATAGAAATGGTTTTTCTAATAAAAATGTTAATAGTCAATATGGTCAAATGGAAATTGACATTCCAAGAGATCGTGAAGCGAAATTTGAACCTATAATCATCAAAAAGTATGAACGTGATATTTCTGAATTAGTTGATATGGTATTTGCTTTATATTCTAGAGGAATGTTAACAAGAGATGTAAGTGATTTTTATGTTTAG", "aa": "MKRKTINPVIDEITEKILENYNPVTSGDLSMAMKEVFQNTIQKMMNKEFDNFMGYEKNDNKVQKNYRNGFSNKNVNSQYGQMEIDIPRDREAKFEPIIIKKYERDISELVDMVFALYSRGMLTRDVSDFYV", "aa_hexdigest": "2221bc545c92d53b9afd41c4f820ce29", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0002DAEC8E", "ncbi_nrp_id": "WP_258408935.1", "uniref100_id": "UniRef100_C4XFF2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002DAEC8E", "RefSeq:WP_258408935.1", "UniRef:UniRef100_C4XFF2" ] }, "ips": { "uniref100_id": "UniRef100_C4XFF2", "uniref90_id": "UniRef90_C4XFF2" }, "psc": { "uniref90_id": "UniRef90_C4XFF2", "product": "Mutator family transposase", "uniref50_id": "UniRef50_C4XFF2" }, "pscc": { "uniref50_id": "UniRef50_C4XFF2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFF2" ], "product": "Mutator family transposase" }, "genes": [], "id": "OAIKOHHJFB_1237", "locus": "GCA000209735_01223" }, { "type": "cds", "sequence": "contig_1", "start": 680807, "stop": 681097, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAACTATTATTACTTTATAGAGTTTATTTTAAAAATTGTTTTACAAGCTTATAAAGAATTTGAAAGCCGTTTTACAAGTTTAGAAAATAAAAAAATTGATGTAGCGGACAACATTTTAAAACTGATAGCTCAATATCAAAAAGTAAGTAAAAATGAGCTAATTAATTTACTACCTGAAAGCAGTAAAAGAACAATTGAAAGAAAACTAAATGTCTTATTAAAAGCTAAAAAAATACTTAAAATTAATCAAGGCAAAAATACTAAATACACTATAAAACAGCATTAA", "aa": "MNNYYYFIEFILKIVLQAYKEFESRFTSLENKKIDVADNILKLIAQYQKVSKNELINLLPESSKRTIERKLNVLLKAKKILKINQGKNTKYTIKQH", "aa_hexdigest": "d10838ab1d375a70a886bae245ca4225", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11452.370099999986, "isoelectric_point": 9.838729667663571 }, "id": "OAIKOHHJFB_1238", "locus": "GCA000209735_01224" }, { "type": "cds", "sequence": "contig_1", "start": 681218, "stop": 681856, "strand": "-", "frame": 1, "gene": null, "product": "Fido domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFF3", "UniRef:UniRef90_C4XFF3" ], "nt": "ATGAAAAATATTGATTATAAAAGAATTTTTAATAAAGGTATCCCCACAAATATTATTAACTTGTTGACTGAAATATATGAATATAAAGGAAGACAAAACATTTATATCAATAACAAGAATTTAGAAATCCAAAAGCTTAAAAAACAAGCTTTAATTGACAGTGCAGTTTATTCAAATAAAATAGAAAACATCAATATAAGCAAAAAAAGAATTAAAGATATTTTGCTTGAAAATGATGAGTTGCAAAATGAAAATGAAGAAGATATTAAAGGTTATTTTGAATTATTAAATTCTCTATATAATCAAGAAACATTTGAATTATTAAACACTAATTTGATTCTTGATCTTCATTATCAATTGTTTCAAAACTCAAATACCAAAATGAGTGGGCGTTTTAAGTTAAATGATAATTTTATTTTAGAAACAACCTTAGAAGGTACTCAAGAAATAAAATTTATTCCTGTCAAAGCATTTTTTATTAGTGAATATATAAATAATTTATGTAATAAATATAATGAAGAATATAAAAGTAAGAAGATTAATTCATTGCTTCTAACAACAGCTTTTGTTTTGGATTTTATTTGCATTTCGCCTTTTAATCACGGCAATAGCAGAATGTCTAGAATTCTTTTAAATTGA", "aa": "MKNIDYKRIFNKGIPTNIINLLTEIYEYKGRQNIYINNKNLEIQKLKKQALIDSAVYSNKIENINISKKRIKDILLENDELQNENEEDIKGYFELLNSLYNQETFELLNTNLILDLHYQLFQNSNTKMSGRFKLNDNFILETTLEGTQEIKFIPVKAFFISEYINNLCNKYNEEYKSKKINSLLLTTAFVLDFICISPFNHGNSRMSRILLN", "aa_hexdigest": "6769c60eb1042ae27a758429ca2b00fb", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 680915, "stop": 681856, "observations": { "mutation": [ 681109, 681220 ], "stop": [ 681109, 681220 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFF3", "query_cov": 1.0, "subject_cov": 0.6074498567335244, "identity": 1.0, "score": 414.0, "evalue": 5.27e-144, "gene_start": 1, "gene_end": 212, "reference_start": 1, "reference_end": 212, "reference_sequence": "MKNIDYKRIFNKGIPTNIINLLTEIYEYKGRQNIYINNKNLEIQKLKKQALIDSAVYSNKIENINISKKRIKDILLENDELQNENEEDIKGYFELLNSLYNQETFELLNTNLILDLHYQLFQNSNTKMSGRFKLNDNFILETTLEGTQEIKFIPVKAFFISEYINNLCNKYNEEYKSKKINSLLLTTAFVLDFICISPFNHGNSRMSRILLNWLLSVGDIKVVQYISLEKIIFENLEKYQNVQQKSSYKWHDNLNNYYYFIEFILKIVLQAYKEFESRFTSLENKKIDVADNILKLIAQYQKVSKNELINLLPESSKRTIERKLNVLLKAKKILKINQGKNTKYTIKQH" }, "paralog": false, "identity": 0.9936102236421726, "score": 610.0, "evalue": 1.07e-223, "description": "Internal stop codon at 681109, 681220. Nonsense mutation around 681109, 681220.", "psc": { "uniref90_id": "UniRef90_C4XFF3", "product": "Fido domain-containing protein", "uniref50_id": "UniRef50_C4XFF3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFF3", "UniRef:UniRef50_C4XFF3" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFF3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFF3" ], "product": "Fido domain-containing protein" } }, "id": "OAIKOHHJFB_1239", "locus": "GCA000209735_01225" }, { "type": "cds", "sequence": "contig_1", "start": 682118, "stop": 682267, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACAAAGTACAAATATGTCGATGTTAATAAAATTATGGACGATCCAAATAAGATTATTAGACATTTAGACATAGATGGTAAAGTAATTGAAAAAAATTACAAAGCACCACTAAGTAATGCTGAAGTTTTAGAAGCTTACAAATGA", "aa": "MKTKYKYVDVNKIMDDPNKIIRHLDIDGKVIEKNYKAPLSNAEVLEAYK", "aa_hexdigest": "ccdf004ab974bcd80bdb658e793756f4", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5753.6466, "isoelectric_point": 8.92650203704834 }, "id": "OAIKOHHJFB_1240", "locus": "GCA000209735_01226" }, { "type": "cds", "sequence": "contig_1", "start": 682268, "stop": 682402, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00676.26" ], "nt": "ATGGTACTATCTAGACAACAAGATACATACATGTTGCAATTGCAACGTCAAGGACGTATGCTTACATTTCCACCTAACTTAGGTGAAGAAGCATTACAAATTGCAACAGCAATGGCTATGGAAAATAAAGACTGA", "aa": "MVLSRQQDTYMLQLQRQGRMLTFPPNLGEEALQIATAMAMENKD", "aa_hexdigest": "ea683447884bb3f934dd9c164b4968d8", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00676.26", "name": "E1_dh", "length": 44, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 1.0119929017482304e-06, "score": 24.01605987548828, "start": 1, "stop": 44 } ], "seq_stats": { "molecular_weight": 5069.836700000002, "isoelectric_point": 5.0568742752075195 }, "id": "OAIKOHHJFB_1241", "locus": "GCA000209735_01227" }, { "type": "cds", "sequence": "contig_1", "start": 682523, "stop": 682690, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00676.26" ], "nt": "ATGCCGGTTAATATTGTTATTGCAACTCAAATTTCACAATGTGCTGGTATTGCTTATGCACAAAAACAACTTAAAACTGGTGGTGTAGCTATTTCATTTATTGGAAATGGCGGAACTACTGAAGGAGAATTTAGCGAAGGTGTTAATATTGCAACTGTTCAAGAATGA", "aa": "MPVNIVIATQISQCAGIAYAQKQLKTGGVAISFIGNGGTTEGEFSEGVNIATVQE", "aa_hexdigest": "d2b0ffa0a9c9fc647b7662acfb9fff92", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00676.26", "name": "E1_dh", "length": 53, "aa_cov": 0.9636363636363636, "hmm_cov": 1.0, "evalue": 2.861736354398763e-12, "score": 42.23418045043945, "start": 2, "stop": 54 } ], "seq_stats": { "molecular_weight": 5613.2896, "isoelectric_point": 4.686511421203612 }, "id": "OAIKOHHJFB_1242", "locus": "GCA000209735_01228" }, { "type": "cds", "sequence": "contig_1", "start": 683230, "stop": 683544, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02779.29" ], "nt": "ATGGCTGAAGAAAAATTAACTTTAAATAATGTTCAAGCAGTTAATAATGCTTTAGACATTGCTATGGCTAAAGACCCCCGTGTAGTTTGTTATGGTGAAGATGCTGGTGTTGAAGGTGGAGTTTTCAGAGCTACAGAAGGCTTACAAAAAAAATATGGTAAAAGTAGAGTATTTGATACCCCTATTTCAGAAGCAACAATTGCTGGTACCGCAATTGGTGCTGCAGTAGCAGGTCTTAGACCTATTGCCGAAATTCAATTCCAAGGTTTTAGTTATCCAGCTATGCAACAATTATTTACTCATGCAGCTAGATGA", "aa": "MAEEKLTLNNVQAVNNALDIAMAKDPRVVCYGEDAGVEGGVFRATEGLQKKYGKSRVFDTPISEATIAGTAIGAAVAGLRPIAEIQFQGFSYPAMQQLFTHAAR", "aa_hexdigest": "0e2861927209bd36f7e5d66cd7ade891", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02779.29", "name": "Transket_pyr", "length": 94, "aa_cov": 0.8942307692307693, "hmm_cov": 0.9787234042553191, "evalue": 9.437133588543148e-22, "score": 73.55757904052734, "start": 10, "stop": 102 } ], "seq_stats": { "molecular_weight": 11074.48989999999, "isoelectric_point": 5.746047019958497 }, "id": "OAIKOHHJFB_1243", "locus": "GCA000209735_01229" }, { "type": "cds", "sequence": "contig_1", "start": 683578, "stop": 684168, "strand": "+", "frame": 1, "gene": null, "product": "Pyruvate dehydrogenase E1 component subunit beta", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2H6AHZ3" ], "nt": "ATGATTTTAAGAATGCCTATGGGTGGTGGAATTAAAGCTATGGAACATCACTCAGAAGCTTTAGAAGCTATTTATGCTCACATTCCAGGTGTTAAAGTTGTTATGCCAGCCTTTCCTTATGATGTTAAAGGTTTATTATTAGCAGCTTTAAATGATCCAGATCCAGTAGTATTCTTAGAAAATAAGAAAATTTATCGTGCTGGGAAACAAGAAGTTCCAGCTGGCGAATATACAGTTGAAATTGGAAAAGCTAATGTCTTAACTCAAGGTAATGACTTAACTTTAGTAACTTATGGAGCTCAAGTATTTGACTCAATTAATGCTGTTAAAAAATATAAAGAAATCAATCCTAACGCTTCAATTGAATTAATTGATTTAAGAACAATTAAACCTCTTGATACTAAAACTATTGTTGAAAGTGTTAAGAAAACAGGACGTTTATTAGTAGTTCATGAAGCTGTTAAATCATTTTCAGTTTCTTCTGAAATTATGGCTAGAGTTAATGAAAAAGCATTTGAATATCTTAAAGCACCTATGACAAGATGTACAGGTTATGATGTAACTGTTCCTCTTGCAAAAGGCGAAGCATGA", "aa": "MILRMPMGGGIKAMEHHSEALEAIYAHIPGVKVVMPAFPYDVKGLLLAALNDPDPVVFLENKKIYRAGKQEVPAGEYTVEIGKANVLTQGNDLTLVTYGAQVFDSINAVKKYKEINPNASIELIDLRTIKPLDTKTIVESVKKTGRLLVVHEAVKSFSVSSEIMARVNEKAFEYLKAPMTRCTGYDVTVPLAKGEA", "aa_hexdigest": "d40c9db939a38851a1139fa7c3de90c4", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_A0A2H6AHZ3", "query_cov": 0.8520408163265306, "subject_cov": 0.8743455497382199, "identity": 0.503, "score": 167.0, "evalue": 3.5e-49, "valid": false, "product": "Pyruvate dehydrogenase E1 component subunit beta", "uniref50_id": "UniRef50_A0A2H6AHZ3" }, "pscc": { "uniref50_id": "UniRef50_A0A2H6AHZ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2H6AHZ3" ], "product": "Pyruvate dehydrogenase E1 component subunit beta" }, "genes": [], "id": "OAIKOHHJFB_1244", "locus": "GCA000209735_01230" }, { "type": "cds", "sequence": "contig_1", "start": 684291, "stop": 684608, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTAAATCAACCCCTATTGCAAGAGCTATGGCTGCTAAACTTGGAATTGATATTAACTTAGTTCCAGGTTCAGGAATTGATGGCCGTATTCTTATAGAAGATATTCAAAAATTTAAAAACAGTCCTATTTCAGGTGCTGCTCATTCAAACTTTGCACCAGCTCCAGTAGCTGATAATAAAGTAATTACTCAACCTAGTGCAGTAGCTCCTAAAGCTACTCCAGCACCTCGTCCTGCCATTGATCGTTCAGCTCACTCAGAAGCTATTACTCCAATTAGAAAAGCAATTTCTAAAGCTATGACTAACTCATGA", "aa": "MKIKSTPIARAMAAKLGIDINLVPGSGIDGRILIEDIQKFKNSPISGAAHSNFAPAPVADNKVITQPSAVAPKATPAPRPAIDRSAHSEAITPIRKAISKAMTNS", "aa_hexdigest": "4cf7105c98f0f1806126bdfc34695a65", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10904.566699999983, "isoelectric_point": 10.28098278045654 }, "id": "OAIKOHHJFB_1245", "locus": "GCA000209735_01231" }, { "type": "cds", "sequence": "contig_1", "start": 684648, "stop": 685160, "strand": "+", "frame": 3, "gene": null, "product": "Peripheral subunit-binding (PSBD) domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B197", "UniRef:UniRef90_C4XFF6" ], "nt": "ATGACTGCTCTTTGGGACTTACGTTCAAGAATCAAAGATATTGTACTTAAAAGTGATAATGTAAAAATTACATTCTTACCTTATATTATTAAAGCAGCTGCTATTGCTTTAAGAGATTTTCCAGTATTCACTGCTAAATATAATGAAGCAAATCAAACTTTAGATTACCCTGGTGTAATTAATGTTGGTATTGCTGTTGATACTGAAGCCGGTTTAATGGTTCCTGTTATTAATAATGCTGATAATTTAAGTATTGTTAATATTGCTAGTGAAGTAACTAGACTTGCAGGCGCTGCAAGAAATAGAACAATTAAACCTGCTGAAATGAAAGGTGCCGGTTTTACTATTACTAACTATGGTTCAGTTGGATCACTTTTTGGAGTTCCTGTTATTAACTATCCAGAATTAGCTATTGGTGGTGTTGGTGCTATTATTGACAAGCCTGTAGTTAAAAATGGACAAGTTGTACCTGGAAAAGTTATGTACCTAACTGTCGCTGCTGATCACCGTTGA", "aa": "MTALWDLRSRIKDIVLKSDNVKITFLPYIIKAAAIALRDFPVFTAKYNEANQTLDYPGVINVGIAVDTEAGLMVPVINNADNLSIVNIASEVTRLAGAARNRTIKPAEMKGAGFTITNYGSVGSLFGVPVINYPELAIGGVGAIIDKPVVKNGQVVPGKVMYLTVAADHR", "aa_hexdigest": "b8d0a3d66b2f42876d1e5f0b879e22bb", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 684348, "stop": 685241, "observations": { "mutation": [ 684606, 685158 ], "stop": [ 684606, 685158 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFF6", "query_cov": 1.0, "subject_cov": 0.5362776025236593, "identity": 1.0, "score": 327.0, "evalue": 3.77e-111, "gene_start": 1, "gene_end": 170, "reference_start": 121, "reference_end": 290, "reference_sequence": "MMKIKSTPIARAMAAKLGIDINLVPGSGIDGRILIEDIQKFKNSPISGAAHSNFAPAPVADNKVITQPSAVAPKATPAPRPAIDRSAHSEAITPIRKAISKAMTNSWSNVAYTNLVHEIDMTALWDLRSRIKDIVLKSDNVKITFLPYIIKAAAIALRDFPVFTAKYNEANQTLDYPGVINVGIAVDTEAGLMVPVINNADNLSIVNIASEVTRLAGAARNRTIKPAEMKGAGFTITNYGSVGSLFGVPVINYPELAIGGVGAIIDKPVVKNGQVVPGKVMYLTVAADHRWIDGAVIGRFASRIKELLEKPDVLGVY" }, "paralog": false, "identity": 0.9932659932659933, "score": 571.0, "evalue": 1.07e-209, "description": "Internal stop codon at 684606, 685158. Nonsense mutation around 684606, 685158.", "psc": { "uniref90_id": "UniRef90_C4XFF6", "product": "Peripheral subunit-binding (PSBD) domain-containing protein", "uniref50_id": "UniRef50_A0A449B197", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFF6", "UniRef:UniRef50_A0A449B197" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449B197", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B197" ], "product": "Pyruvate dehydrogenase E2 component (Dihydrolipoamide acetyltransferase)" } }, "id": "OAIKOHHJFB_1246", "locus": "GCA000209735_01232" }, { "type": "cds", "sequence": "contig_1", "start": 685243, "stop": 686151, "strand": "+", "frame": 1, "gene": "lpdA", "product": "dihydrolipoyl dehydrogenase", "db_xrefs": [ "EC:1.8.1.4", "GO:0004148", "GO:0050660", "KEGG:K00382", "SO:0001217", "UniRef:UniRef50_A0A449B5U3", "UniRef:UniRef90_C4XFF7" ], "nt": "ATGTACGTATTTAAATTTGCTGATATAGGTGAAGGATTACACGAAGGAACAGTTGGTGAAATCAGTGTTAAAGTTGGTGACAAGATTAAAGAAGGAGATACACTTTTTTCAGTAGAAACTGATAAAGTTGCTTCTGAAATTCCTTCTCCAGTAGATGGAATAATCAAAGAAATTAGAATGAAGACTGGTGATGTAATTCATGTAGGACAAGAAATATTTGTAATTGATGATGGCAAAGGTGGCTCAGTAGCTGCAGCTCCTGCTACTAAAGTTGAAGAATCGAAAAGTAGACCTGCTACTTCATCATCTACAAGCTTTTATGTTTTTAAATTTGCTGACATTGGTGAAGGTTTACACGAAGGAACAGTTGGTGAAATTCCTATTAAAATAGGTGATAAAGTAAAAGAAGGCGACACATTATTCTCAGTAGAAACTGATAAAGTTGCTTCTGAAATTCCTTCTCCAGTAGATGGAATAATCAAAGAAATTAAAATGAAAACAGGTGATGTAATTCATGTAGGACAAGAAGTTATTGTTATTGATGCAGGAAGTAAAAATCCTACTTCTTCTTCAGTTTCATCTGCACCAAGTTCCAATAAAGAAGAAGAAAAATGTTTAGTAGGTGATGCTCCAAATTCAAGTGAATTAATAAATCTAGATTTCAGTTCACTAAATTCACCCGCAAAAGAAACTCCTCAACCAGTTGCACCTCAAGCTTCAACTTATGAAGCTAACCAAGAAGAAGAAAGCAATGGTGTTTCATTTACTTCTGAAGGTAAAAAATACGAAGGCAAAGTTGATGAAGAATTTGATGTAATTATTGTAGGTAGTGGTCCTGGAGGATATTTAGCCGCTGAAGAATTAGGCAAAGCAGGCAAAAAAGTATTAATTGTTGAAAAAGAATTCTGA", "aa": "MYVFKFADIGEGLHEGTVGEISVKVGDKIKEGDTLFSVETDKVASEIPSPVDGIIKEIRMKTGDVIHVGQEIFVIDDGKGGSVAAAPATKVEESKSRPATSSSTSFYVFKFADIGEGLHEGTVGEIPIKIGDKVKEGDTLFSVETDKVASEIPSPVDGIIKEIKMKTGDVIHVGQEVIVIDAGSKNPTSSSVSSAPSSNKEEEKCLVGDAPNSSELINLDFSSLNSPAKETPQPVAPQASTYEANQEEESNGVSFTSEGKKYEGKVDEEFDVIIVGSGPGGYLAAEELGKAGKKVLIVEKEF", "aa_hexdigest": "6f9eccf1b22e5c5631c109bf3544958a", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "lpdA" ], "truncated": "3-prime", "pseudogene": { "start": 685237, "stop": 686454, "observations": { "mutation": [ 686149, 686173, 686293 ], "stop": [ 686149, 686173, 686293 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFF7", "query_cov": 1.0, "subject_cov": 0.4092140921409214, "identity": 1.0, "score": 511.0, "evalue": 2.86e-175, "gene_start": 1, "gene_end": 302, "reference_start": 3, "reference_end": 304, "reference_sequence": "MNMYVFKFADIGEGLHEGTVGEISVKVGDKIKEGDTLFSVETDKVASEIPSPVDGIIKEIRMKTGDVIHVGQEIFVIDDGKGGSVAAAPATKVEESKSRPATSSSTSFYVFKFADIGEGLHEGTVGEIPIKIGDKVKEGDTLFSVETDKVASEIPSPVDGIIKEIKMKTGDVIHVGQEVIVIDAGSKNPTSSSVSSAPSSNKEEEKCLVGDAPNSSELINLDFSSLNSPAKETPQPVAPQASTYEANQEEESNGVSFTSEGKKYEGKVDEEFDVIIVGSGPGGYLAAEELGKAGKKVLIVEKEFWGGVCLNIWCIPTKAMLKSTDVLETLTDAAGYGIVGNLDKLKIDLQKTWVKMHERKKGVVDQISSSVKKLMIASKCKIEEGEAEFVGAHEIKINGKVYRGTNIILATGSHSRRLRALPGFKVGYENNYVLSSREAINYDSKLPGSVVIVGGGVVGVEFAQVFASAGAKVTIIQNQNHLLPGIDHDVTNEILKHLEKHGVQIIYNATSTGLNNKKELLYEIGGKERKIKADVYLIAVGRIPSSKGIAEVGVNVGVREEVLVDEKMRTNVKGVYAIGDLTGQNMLAHVAYQHALIAVGNILGEKNVRYHNKPVPGCIYTNTEIAFIGLTEEEAKNKGYNIFTSKYMFSYLGKAIATKQTSGFVKLVVDREYGQILGAHIIGAHATDYISEIALAMEQEVSVKELAYTIHPHPTYSEIIWEAARAASLKLYLEENKK" }, "paralog": false, "identity": 0.9901234567901235, "score": 719.0, "evalue": 1.86e-259, "description": "Internal stop codon at 686149, 686173, 686293. Nonsense mutation around 686149, 686173, 686293.", "psc": { "uniref90_id": "UniRef90_C4XFF7", "gene": "lpdA", "product": "dihydrolipoyl dehydrogenase", "ec_ids": [ "1.8.1.4" ], "uniref50_id": "UniRef50_A0A449B5U3", "kegg_orthology_id": "K00382", "go_ids": [ "GO:0004148", "GO:0050660" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFF7", "EC:1.8.1.4", "UniRef:UniRef50_A0A449B5U3", "KEGG:K00382", "GO:0004148", "GO:0050660" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449B5U3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B5U3" ], "product": "Dihydrolipoyl dehydrogenase" } }, "id": "OAIKOHHJFB_1247", "locus": "GCA000209735_01233" }, { "type": "cds", "sequence": "contig_1", "start": 686194, "stop": 686295, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAATCAACTGATGTTTTAGAAACCTTAACCGATGCTGCTGGTTATGGTATTGTTGGGAACTTAGATAAATTAAAGATAGATCTACAAAAAACATGA", "aa": "MLKSTDVLETLTDAAGYGIVGNLDKLKIDLQKT", "aa_hexdigest": "f965803749b04d3a897180f271242a87", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3565.094500000001, "isoelectric_point": 4.777965354919433 }, "id": "OAIKOHHJFB_1248", "locus": "GCA000209735_01234" }, { "type": "cds", "sequence": "contig_1", "start": 686302, "stop": 687399, "strand": "+", "frame": 1, "gene": null, "product": "Dihydrolipoyl dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223MB31" ], "nt": "ATGCATGAACGTAAAAAAGGCGTAGTAGATCAAATTTCAAGTAGTGTTAAAAAATTAATGATTGCTTCTAAATGTAAAATAGAAGAAGGCGAAGCAGAATTTGTTGGAGCTCACGAAATTAAAATAAATGGTAAAGTATATCGTGGCACAAATATTATTTTAGCCACAGGCAGTCACTCACGTAGATTGCGTGCTTTACCTGGATTTAAGGTTGGATATGAAAATAATTATGTTCTTTCAAGCCGCGAAGCAATTAACTATGATTCAAAATTGCCTGGATCGGTAGTTATTGTTGGTGGTGGTGTTGTTGGCGTTGAATTTGCTCAAGTATTTGCTTCAGCAGGTGCTAAAGTAACTATTATTCAAAACCAAAACCACTTACTTCCTGGTATTGATCATGATGTAACAAATGAAATTCTTAAACATCTAGAAAAGCATGGGGTTCAAATTATTTACAATGCAACTTCAACAGGTTTAAATAATAAAAAAGAACTTCTTTACGAAATTGGTGGTAAAGAAAGAAAAATTAAAGCTGATGTTTATTTGATTGCTGTTGGACGTATTCCATCAAGTAAAGGTATTGCAGAAGTTGGAGTTAATGTCGGAGTTAGAGAGGAAGTTCTAGTTGATGAAAAAATGAGAACAAATGTTAAAGGCGTTTATGCAATTGGAGATTTAACTGGACAAAACATGTTAGCTCACGTTGCATATCAACATGCTTTAATTGCTGTTGGTAACATTTTAGGTGAAAAAAATGTTCGTTATCACAATAAACCAGTTCCAGGATGTATTTATACAAACACTGAAATTGCTTTCATTGGTTTAACTGAAGAAGAAGCAAAAAATAAAGGATACAATATTTTTACTTCAAAATACATGTTCTCTTATTTAGGAAAAGCTATTGCAACCAAACAAACTTCTGGTTTTGTTAAATTAGTTGTTGATCGTGAATATGGGCAAATATTAGGAGCTCACATTATTGGAGCTCATGCAACTGACTATATTTCGGAAATAGCACTTGCTATGGAACAAGAAGTTAGTGTCAAAGAATTAGCTTACACAATTCACCCACACCCAACTTATTCGGAAATTATTTGA", "aa": "MHERKKGVVDQISSSVKKLMIASKCKIEEGEAEFVGAHEIKINGKVYRGTNIILATGSHSRRLRALPGFKVGYENNYVLSSREAINYDSKLPGSVVIVGGGVVGVEFAQVFASAGAKVTIIQNQNHLLPGIDHDVTNEILKHLEKHGVQIIYNATSTGLNNKKELLYEIGGKERKIKADVYLIAVGRIPSSKGIAEVGVNVGVREEVLVDEKMRTNVKGVYAIGDLTGQNMLAHVAYQHALIAVGNILGEKNVRYHNKPVPGCIYTNTEIAFIGLTEEEAKNKGYNIFTSKYMFSYLGKAIATKQTSGFVKLVVDREYGQILGAHIIGAHATDYISEIALAMEQEVSVKELAYTIHPHPTYSEII", "aa_hexdigest": "bd6781793d9af842dfb6f9d3e85b2a63", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A223MB31", "query_cov": 0.8602739726027397, "subject_cov": 0.9661538461538461, "identity": 0.51, "score": 312.0, "evalue": 9.29e-102, "valid": false, "product": "Dihydrolipoyl dehydrogenase", "uniref50_id": "UniRef50_A0A223MB31" }, "pscc": { "uniref50_id": "UniRef50_A0A223MB31", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223MB31" ], "product": "Dihydrolipoyl dehydrogenase" }, "genes": [], "id": "OAIKOHHJFB_1249", "locus": "GCA000209735_01235" }, { "type": "cds", "sequence": "contig_1", "start": 687996, "stop": 688238, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00145.23" ], "nt": "ATGACTAGTAAAAAAGCTATTAGTAGCTTAAAAAACAAAAGTTATGAACTTAAAAAAGCCCTAGTAGCTGCAAATCTAATATCAAATAATTTTAATGATATTACAAAGATTACTAAAGAACAATTAGATCAGTTACATTTCGATTTAATAACTTATTCATTCCCTTGCCAAGGTCTTTCAAGTGCTAATATGGGTCGTTCGTTAGGAATTAAAAACAACAATTCAACTAGTCATTTAGTTTGA", "aa": "MTSKKAISSLKNKSYELKKALVAANLISNNFNDITKITKEQLDQLHFDLITYSFPCQGLSSANMGRSLGIKNNNSTSHLV", "aa_hexdigest": "486cbdf74bdb9759525870d8ecd6f476", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00145.23", "name": "DNA_methylase", "length": 40, "aa_cov": 0.5, "hmm_cov": 0.975, "evalue": 7.69827765209853e-10, "score": 34.73849868774414, "start": 24, "stop": 63 } ], "seq_stats": { "molecular_weight": 8846.046199999995, "isoelectric_point": 9.61212329864502 }, "id": "OAIKOHHJFB_1250", "locus": "GCA000209735_01236" }, { "type": "cds", "sequence": "contig_1", "start": 688635, "stop": 688991, "strand": "+", "frame": 3, "gene": null, "product": "DNA cytosine methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003080B288" ], "nt": "ATGGCTTTTGAAAATAAAAATTATTCAACTATTTATAAGAATCAAAAGATTGATGAATGTCGTATTAACACATTGACAACCAAACAAGATCGTCATCCTAATGTTGGGGTTTTAGATTATGATGCTAATTTAAAAGGTTTTTATAACAAAAGATTTGTAACAAATAGAGAAGCTTACAAAATTATGGGATTTGAAGATAAAGATTTTGAAAAAGTTAATGAATTTAAGAAAAAAAACATAATAACAAAAGAAGCTCTTTATCGTCAAGCAGGCAATTCAATTTGTGTGCCAGCTATAAAAAGTGTATTTCAGTTAATAGCAAAAATCGAAGAATATAATGGAGGTCAAAATGATTAG", "aa": "MAFENKNYSTIYKNQKIDECRINTLTTKQDRHPNVGVLDYDANLKGFYNKRFVTNREAYKIMGFEDKDFEKVNEFKKKNIITKEALYRQAGNSICVPAIKSVFQLIAKIEEYNGGQND", "aa_hexdigest": "fbec0743b0d70735c4ada3083e8f725b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003080B288", "query_cov": 0.9152542372881356, "subject_cov": 0.9908256880733946, "identity": 0.519, "score": 107.0, "evalue": 5.47e-28, "valid": false, "product": "DNA cytosine methyltransferase", "uniref50_id": "UniRef50_UPI003080B288" }, "pscc": { "uniref50_id": "UniRef50_UPI003080B288", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003080B288" ], "product": "DNA cytosine methyltransferase" }, "genes": [], "id": "OAIKOHHJFB_1251", "locus": "GCA000209735_01237" }, { "type": "cds", "sequence": "contig_1", "start": 689272, "stop": 689493, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGTTTTAGAAGAATTAAAAATATTAGATAATTTTCATGAAAATTATTACAAATTCGACGAAGATTTTATTATTGAATTAAAAGCTTATTTTGGAAGTAACTTAGAAAACGAATTAAACTATAAAAAAGTAGATTTGGAAAAATTTGTATATAAATATATTTTGCGAAATGTTAACAAAAAATTAAAACAAAATTTAATTAAAAAATTAAATAAATGA", "aa": "MEVLEELKILDNFHENYYKFDEDFIIELKAYFGSNLENELNYKKVDLEKFVYKYILRNVNKKLKQNLIKKLNK", "aa_hexdigest": "6a3ddd0d2dca754a56ee1c1e85ca5449", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9023.364699999993, "isoelectric_point": 7.862902641296388 }, "id": "OAIKOHHJFB_1252", "locus": "GCA000209735_01238" }, { "type": "cds", "sequence": "contig_1", "start": 689659, "stop": 689979, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCTTATTATAAGGAATTAATAAACAAAATTGAATTAAATAATGAAAACACAAATTTAAATTCTTTTAATGAAAATATGGATGAATTATTAAACATTTTAAACGCTAAAAAAGATGATGATATAAGTAATATTTTATATACAAATGAAGAAGCAATTGTTACAAAAGATTTGGATAGAAATAAATTTTATATTGATGATAATGAAGCAATTTATAAAGAATTAGCTCCTTCTTTTGATGTATTAAATATTCAATCATATTTTAATAAACTTTTAAATATGAAGATATCTATACCAGTATTTCAAAGAAGTTACGTATGA", "aa": "MSYYKELINKIELNNENTNLNSFNENMDELLNILNAKKDDDISNILYTNEEAIVTKDLDRNKFYIDDNEAIYKELAPSFDVLNIQSYFNKLLNMKISIPVFQRSYV", "aa_hexdigest": "9aeba6105ae60bbc1a27f0f1694fcf82", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12539.965599999989, "isoelectric_point": 4.467908668518065 }, "id": "OAIKOHHJFB_1253", "locus": "GCA000209735_01239" }, { "type": "cds", "sequence": "contig_1", "start": 690136, "stop": 690360, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACATTAATATTAATTGCATATTCTTTATATAAATTGTTTTTACATGCTAAAAATTTGAAATTATTTTTACCAAAAGATCCTGTCGAAAATATGTTTAGATATTACATTGATATAAAAGACAATTCTAATAGTTTATTGCCTAGAAAAATAACAAGTATTTATAACAATATTAGTGAAAGTGATTCATACAATTCTCTTAGCATCGCTTTAGGTGATAATTGA", "aa": "MTLILIAYSLYKLFLHAKNLKLFLPKDPVENMFRYYIDIKDNSNSLLPRKITSIYNNISESDSYNSLSIALGDN", "aa_hexdigest": "5dd3def6fe32bacddafe3c2f02afe84d", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8575.815299999995, "isoelectric_point": 7.963795661926269 }, "id": "OAIKOHHJFB_1254", "locus": "GCA000209735_01240" }, { "type": "cds", "sequence": "contig_1", "start": 690586, "stop": 690858, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTCAAAACCATTAACGTCATACGATTTAGTTCGTAATTTTGTTTATGGTCAACTAAGTGAAAAAAGTTCAAATGTAGAATCAAACATTAGCAAATTTGACAATTTCTTTAAACCATTATTTATTAGTAAAAGTGAAAGAAAAACACTTGTCTTGGATTCAAAATTGTTTGATAATTACGTTAACTCAATGGCAACTATTAATAACGTATCAATTAATTCAAATGAATTATCAAACAATTTAACCTACAATATTTTTGTAGAAGTTTGA", "aa": "MYSKPLTSYDLVRNFVYGQLSEKSSNVESNISKFDNFFKPLFISKSERKTLVLDSKLFDNYVNSMATINNVSINSNELSNNLTYNIFVEV", "aa_hexdigest": "7c943b39fd45e745b988763a6e7e5306", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10376.561799999992, "isoelectric_point": 6.161540031433105 }, "id": "OAIKOHHJFB_1255", "locus": "GCA000209735_01241" }, { "type": "cds", "sequence": "contig_1", "start": 691084, "stop": 691494, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTATTTAATGTCAATTTGGTCAAAGAATCGAGAATTAGTTTACTAAGAAGATTATCAAAAGTAGTTTATAAAGTTGCTAGAATTAGATTCTTTAACAAATTTATTTCATTTAAAGGTGAATCACTAACAATGCCAATTAAAAAAATAGTTCAAAGTTTAAATGATGACTGTACAAACTTTGATAAATTAGCATACAATAATTTTGTATTTTTAAGAAAATATGCTGAAATTGATGAAATAAATAAAAAGATGGAACAAGATTTGTTAAATAAGAAAACAGATTTATTAGCATCTTCAGACGATCCAGTTTCTTTATTACTTTTAATAAATTATAGTATAGGAAAAAATAATAAAAATCTACACTATTTTGAAAATGATGAACAATCTTCTTATTTTAAAGATTGA", "aa": "MNLFNVNLVKESRISLLRRLSKVVYKVARIRFFNKFISFKGESLTMPIKKIVQSLNDDCTNFDKLAYNNFVFLRKYAEIDEINKKMEQDLLNKKTDLLASSDDPVSLLLLINYSIGKNNKNLHYFENDEQSSYFKD", "aa_hexdigest": "73f666d7ac5f54d826365e1116d9d8af", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16030.319399999978, "isoelectric_point": 9.254775047302243 }, "id": "OAIKOHHJFB_1256", "locus": "GCA000209735_01242" }, { "type": "cds", "sequence": "contig_1", "start": 691954, "stop": 692436, "strand": "+", "frame": 1, "gene": null, "product": "C4-dicarboxylate transporter/malic acid transport protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFG0", "UniRef:UniRef90_C4XFG0" ], "nt": "ATGACTTTTAAAAATAAATTAGAAAAAGTACCTCTTGGTTTAGGAGGACTTGCACTTGGCATAAGTGGTTTAGCTGGTGCTTATAATGGGGCTTTTAATGAATTTAAAAACTCAAGCAATGGAAAATTAATTAATAATGTTTCAATTGGCATTCAAGCTTTTTGGATGTTAATTGCTTTTGCTTTAGCTTTTATTGTTTGCTTAAGATTTATTAAAGCAAGGCACGTATTTAATAAAGAAGCAAAAGCTCCAAATACAGCAGCATTTATACCCACTTTATTAATGACATTTATTTCTATTTTTACTTTTATAGCCTCAATATTAAATACTTATTTAGAAGCTAGTGAAGCTAAAAAATGGTTATATATTCCATTGGTATTTTCATTCATTGCTTTTATATTACAAATAGCTTATTTAATAACTTTTATAGTATTAATTATAGTTCAACATGATTTTAAATATGATGAAGCTTTTGCTTCTTGA", "aa": "MTFKNKLEKVPLGLGGLALGISGLAGAYNGAFNEFKNSSNGKLINNVSIGIQAFWMLIAFALAFIVCLRFIKARHVFNKEAKAPNTAAFIPTLLMTFISIFTFIASILNTYLEASEAKKWLYIPLVFSFIAFILQIAYLITFIVLIIVQHDFKYDEAFAS", "aa_hexdigest": "8072d51ff5118b8e2ac614d4681ec806", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 691954, "stop": 692739, "observations": { "mutation": [ 692434, 692524 ], "stop": [ 692434, 692524 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFG0", "query_cov": 1.0, "subject_cov": 0.4134366925064599, "identity": 1.0, "score": 303.0, "evalue": 1.27e-100, "gene_start": 1, "gene_end": 160, "reference_start": 1, "reference_end": 160, "reference_sequence": "MTFKNKLEKVPLGLGGLALGISGLAGAYNGAFNEFKNSSNGKLINNVSIGIQAFWMLIAFALAFIVCLRFIKARHVFNKEAKAPNTAAFIPTLLMTFISIFTFIASILNTYLEASEAKKWLYIPLVFSFIAFILQIAYLITFIVLIIVQHDFKYDEAFASWFIPTVGFVILTFGANNYQNIIPLLFYQILWFIGLLFCFLIFILVAFKVAFVGHKNHQDIPSLAIFCAPAMVLSFAFLTLFDPKNPNANILNNYVILYTFTGILFLFILVGWAAYFYSLAISIKSKKFYFTWACFTFPIEISSTALFKYAQVVFTKDLNVYVHFAFIILAILFLIFATVIINYVFYKYVVGLHKIFSDKDKNNSDIQNQKTIVEGIEVTKVDLDSLE" }, "paralog": false, "identity": 0.9923371647509579, "score": 509.0, "evalue": 2.08e-184, "description": "Internal stop codon at 692434, 692524. Nonsense mutation around 692434, 692524.", "psc": { "uniref90_id": "UniRef90_C4XFG0", "product": "C4-dicarboxylate transporter/malic acid transport protein", "uniref50_id": "UniRef50_C4XFG0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFG0", "UniRef:UniRef50_C4XFG0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFG0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFG0" ], "product": "C4-dicarboxylate transporter/malic acid transport protein" } }, "id": "OAIKOHHJFB_1257", "locus": "GCA000209735_01243" }, { "type": "cds", "sequence": "contig_1", "start": 692644, "stop": 692769, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTATTAAGTTTTGCATTTTTAACTTTATTTGATCCTAAGAATCCAAACGCAAATATTTTAAATAATTATGTAATTTTATATACTTTTACAGGAATATTATTCTTATTTATTTTAGTTGGTTGA", "aa": "MVLSFAFLTLFDPKNPNANILNNYVILYTFTGILFLFILVG", "aa_hexdigest": "f9947ede4f3897ac5b704ebd3b555576", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4668.535199999998, "isoelectric_point": 5.587011528015137 }, "id": "OAIKOHHJFB_1258", "locus": "GCA000209735_01244" }, { "type": "cds", "sequence": "contig_1", "start": 692887, "stop": 693117, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32FA1", "UniRef:UniRef90_UPI0001E32FA1" ], "nt": "GTGGTATTTACTAAGGATTTAAATGTTTATGTTCATTTTGCTTTTATTATTTTAGCTATCTTATTCTTAATCTTTGCAACAGTAATTATTAATTATGTTTTCTATAAATATGTTGTTGGTTTACATAAAATATTTAGCGATAAAGATAAAAACAATTCTGATATTCAAAATCAAAAAACAATTGTTGAAGGAATTGAAGTTACTAAAGTTGACTTAGATTCTTTAGAGTAA", "aa": "MVFTKDLNVYVHFAFIILAILFLIFATVIINYVFYKYVVGLHKIFSDKDKNNSDIQNQKTIVEGIEVTKVDLDSLE", "aa_hexdigest": "76c01552a3dd2aa9b2239952e9e571be", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 692644, "stop": 693117, "observations": { "mutation": [ 692767, 692827 ], "stop": [ 692767, 692827 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001E32FA1", "query_cov": 0.9868421052631579, "subject_cov": 0.47770700636942676, "identity": 1.0, "score": 138.0, "evalue": 2.94e-40, "gene_start": 2, "gene_end": 76, "reference_start": 83, "reference_end": 157, "reference_sequence": "MVLSFAFLTLFDPKNPNANILNNYVILYTFTGILFLFILVGWAAYFYSLAISIKSKKFYFTWACFTFPIAISSTALFKYAQVVFTKDLNVYVHFAFIILAILFLIFATVIINYVFYKYVVGLHKIFSDKDKNNSDIQNQKTIVEGIEVTKVDLDSLE" }, "paralog": false, "identity": 0.9808917197452229, "score": 300.0, "evalue": 1.36e-106, "description": "Internal stop codon at 692767, 692827. Nonsense mutation around 692767, 692827.", "psc": { "uniref90_id": "UniRef90_UPI0001E32FA1", "uniref50_id": "UniRef50_UPI0001E32FA1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001E32FA1", "UniRef:UniRef50_UPI0001E32FA1" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32FA1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32FA1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8816.216200000003, "isoelectric_point": 5.5337533950805655 }, "id": "OAIKOHHJFB_1259", "locus": "GCA000209735_01245" }, { "type": "cds", "sequence": "contig_1", "start": 693183, "stop": 693395, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF45E34", "UniRef:UniRef90_UPI000FF45E34" ], "nt": "GTGCTAAATGATATAAATTCTCAAGAAATAACAATTTTGCAAATTCCAGCCAAAAAATTTGAAATTTCAGATACTAAAGGAACGAACAAATTTCAAAAAACTAGAAGTGATAATAAATTTGATTTATATATAAATAATGTTTTATTCGAAAAACAAAATAAAATTGACTTTACAGAATATGTAATAGATAAAATTCGTTATTCTTCCTCATAA", "aa": "MLNDINSQEITILQIPAKKFEISDTKGTNKFQKTRSDNKFDLYINNVLFEKQNKIDFTEYVIDKIRYSSS", "aa_hexdigest": "dceb99835d3e0becdfbec2a52cad4861", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000FF45E34", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.9570000000000001, "score": 130.0, "evalue": 2.71e-38, "valid": true, "uniref50_id": "UniRef50_UPI000FF45E34" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF45E34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF45E34" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8281.2759, "isoelectric_point": 7.942907905578614 }, "id": "OAIKOHHJFB_1260", "locus": "GCA000209735_01246" }, { "type": "cds", "sequence": "contig_1", "start": 693954, "stop": 694160, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTAATCTGCGATAATTTAAGCATGACAACAAATACTAATAAAAATTCTTTAACAACAACCATTCAATTAATATTAGAAGATGGGACATTAGACGGGATTTTGTATCTCAAAACTTTAAGATGGCCTCTAGGAGGAATTATGTTGGTAAGTCCTCGCGATAAGGTTACTAAATTATTGGACGAATATGAAGAATGCAATAATTGA", "aa": "MLICDNLSMTTNTNKNSLTTTIQLILEDGTLDGILYLKTLRWPLGGIMLVSPRDKVTKLLDEYEECNN", "aa_hexdigest": "991e593e6f90afe58f95af4846ab850a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7688.845199999999, "isoelectric_point": 4.563057136535646 }, "id": "OAIKOHHJFB_1261", "locus": "GCA000209735_01247" }, { "type": "cds", "sequence": "contig_1", "start": 694264, "stop": 694749, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013527078.1", "SO:0001217", "UniParc:UPI0001F5988B", "UniRef:UniRef100_A0AB32XCW2", "UniRef:UniRef50_C4XFG2", "UniRef:UniRef90_C4XFG2" ], "nt": "GTGAAACTATCGATCAAAGAAATTATCAAGAGAATTAAAATTCTTGAGGACGAATTAATTGTTCTTAATCAAGAAGAAGATAAAGATAATTATGTTATATATTTAAAAGATGAAACAGTTGAAAAATCATCGTATAATTTTAATAAAACTAATAAAAAACGTCAAGAAATTAATCAAGAAATTTTAAGACTTAAAATTATTGTTCAAAAAGCAAACATCAAAACAATAACTAGTTATAAAGGATTAAGTATTTCTGAACTTTTAATTTTATTAGCACAAAAATCTTCTTTAGTTCAAAGATTTGATTTGTTATGTTCTAAAAAGCAAAAAACAAGAAAAACTACCAACGATGGTCAAATTGAATATATTGAATATTTATATGATATTAACGAATTAAAAAATACTAATTTAAAATTAAAACAAGAAATAGCAACAATGCAAGTTGCTATTGATAAAGCTAATATTGAAACATTAATTGAAATATAA", "aa": "MKLSIKEIIKRIKILEDELIVLNQEEDKDNYVIYLKDETVEKSSYNFNKTNKKRQEINQEILRLKIIVQKANIKTITSYKGLSISELLILLAQKSSLVQRFDLLCSKKQKTRKTTNDGQIEYIEYLYDINELKNTNLKLKQEIATMQVAIDKANIETLIEI", "aa_hexdigest": "20c7a4d9986065e034274e9e251fc3fc", "start_type": "GTG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001F5988B", "ncbi_nrp_id": "WP_013527078.1", "uniref100_id": "UniRef100_A0AB32XCW2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5988B", "RefSeq:WP_013527078.1", "UniRef:UniRef100_A0AB32XCW2" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCW2", "uniref90_id": "UniRef90_C4XFG2" }, "psc": { "uniref90_id": "UniRef90_C4XFG2", "uniref50_id": "UniRef50_C4XFG2" }, "pscc": { "uniref50_id": "UniRef50_C4XFG2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFG2" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18910.837600000003, "isoelectric_point": 8.96382923126221 }, "id": "OAIKOHHJFB_1262", "locus": "GCA000209735_01248" }, { "type": "cds", "sequence": "contig_1", "start": 694928, "stop": 695137, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_129622071.1", "SO:0001217", "UniParc:UPI000FF6D7AB", "UniRef:UniRef100_A0AB32XD63", "UniRef:UniRef50_A0AB32XD63", "UniRef:UniRef90_A0AB32XD63" ], "nt": "ATGGAAGTAAAGGGTTATACATATTTAGCTACATCTATGATGTGGCTTTTTTATTCTAAATTTAAATCAGATGCTTTTACTCCATTTTTACTTTTCATTTTTTCTAAAATAGTTTTAAGTTTATTTAATAATAATTCAATTCTTAATTTGTCTTATGGAAAAGATTATTTTATAGAAAATATAGATAATCCAAAATTTCTTAAAATATAA", "aa": "MEVKGYTYLATSMMWLFYSKFKSDAFTPFLLFIFSKIVLSLFNNNSILNLSYGKDYFIENIDNPKFLKI", "aa_hexdigest": "2a3110a198dad2a49e4108e22362e97d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000FF6D7AB", "ncbi_nrp_id": "WP_129622071.1", "uniref100_id": "UniRef100_A0AB32XD63", "db_xrefs": [ "SO:0001217", "UniParc:UPI000FF6D7AB", "RefSeq:WP_129622071.1", "UniRef:UniRef100_A0AB32XD63" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XD63", "uniref90_id": "UniRef90_A0AB32XD63" }, "psc": { "uniref90_id": "UniRef90_A0AB32XD63", "uniref50_id": "UniRef50_A0AB32XD63" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XD63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XD63" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8182.522899999993, "isoelectric_point": 8.963700294494632 }, "id": "OAIKOHHJFB_1263", "locus": "GCA000209735_01249" }, { "type": "cds", "sequence": "contig_1", "start": 695146, "stop": 695295, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGACTGTCGCCCAAGTGGTGCTTATGAACTTCATCAAAAACTAAATAATTCGCATTTAATTATGGTTGAAAAAGCAGCTCATTCACAAAGAGAAGTTAATATAACTAAGGCTTTAGTTAAAATTACAGATCAAATTAGAAAAAAATAG", "aa": "MDCRPSGAYELHQKLNNSHLIMVEKAAHSQREVNITKALVKITDQIRKK", "aa_hexdigest": "7ea3557e6a9d014839986bf3c9b253e5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5644.516, "isoelectric_point": 9.652931785583494 }, "id": "OAIKOHHJFB_1264", "locus": "GCA000209735_01250" }, { "type": "cds", "sequence": "contig_1", "start": 695827, "stop": 696090, "strand": "-", "frame": 2, "gene": null, "product": "prolyl aminopeptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V9FP21" ], "nt": "ATGTACAAAAAATATTTATATCCAAAAATTGAACCTTATCAAAAAGGTTATTTAAAAGTAGATGATATTTATCAAATTTATTATGAAGTTAGTGGCAATCCACAAGGTCAACCAATAGTTTATGTTCATGGTGGTCCTGGTGGAGGAACTAGTGAAGTATGTAGAAGATATTTTGACCCAAAATACTATAAAATAGTTTTATTTGACCAAAGAGGTTGCGGAAAAAGTACTCCAAGTTTAGAGTTAAAAAATAATACAACTTGA", "aa": "MYKKYLYPKIEPYQKGYLKVDDIYQIYYEVSGNPQGQPIVYVHGGPGGGTSEVCRRYFDPKYYKIVLFDQRGCGKSTPSLELKNNTT", "aa_hexdigest": "3de01bb3ebf32601049827166c585ef4", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_UPI0017C9CCE2", "query_cov": 0.9770114942528736, "subject_cov": 0.9042553191489362, "identity": 0.612, "score": 122.0, "evalue": 8.64e-35, "valid": false, "product": "alpha/beta fold hydrolase", "uniref50_id": "UniRef50_A0A7V9FP21" }, "pscc": { "uniref50_id": "UniRef50_A0A7V9FP21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V9FP21" ], "product": "prolyl aminopeptidase" }, "genes": [], "id": "OAIKOHHJFB_1265", "locus": "GCA000209735_01251" }, { "type": "cds", "sequence": "contig_1", "start": 696142, "stop": 696627, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13749.12", "PFAM:PF08279.18", "PFAM:PF08220.18" ], "nt": "TTGAGTGGCGTACCAACTATCCAACAATTTGAAAATAGAATTGAAATTATGTCTTATGGTGGATTACCAAAAGGTTTGAAACTTGAAGAATTTTATCAAGGAATAAGTAAGCCAAGAAATCCAAAATTAATGAAAATATTTATAGATCTTAATTTGGCAGAACAAACCGGACATGGCGTTCCACTTATTCTAAAAAAATATGGAAAAATGGCAATTGAAGTAGAAAAAAACTATATCAGGATAACAATACCTTTTAACAAGGAATTGCTACAAAAAAATAACAATTATGAAATTAAAGAAATAAAAGACAAATTAAACAAAATCGAAAATCAAATATTCGAATTAATTCAAAAAAATGAAGAATATACAGCTTATGAATTGTCAAACGAATTAGAAGTTTCTAAAAGAACAATAGAAAGAGCTTTAGCATCTTTAAAAAACAAACTAATTTTAAAAAGAATTGGTTCAAGAAAAACAGGAAAATGA", "aa": "MSGVPTIQQFENRIEIMSYGGLPKGLKLEEFYQGISKPRNPKLMKIFIDLNLAEQTGHGVPLILKKYGKMAIEVEKNYIRITIPFNKELLQKNNNYEIKEIKDKLNKIENQIFELIQKNEEYTAYELSNELEVSKRTIERALASLKNKLILKRIGSRKTGK", "aa_hexdigest": "664f051e93aff4d5ed5fb5b280f5b65a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13749.12", "name": "HATPase_c_4", "length": 66, "aa_cov": 0.39751552795031053, "hmm_cov": 1.0, "evalue": 2.0905042157279064e-15, "score": 52.635982513427734, "start": 6, "stop": 69 }, { "id": "PF08279.18", "name": "HTH_11", "length": 41, "aa_cov": 0.2484472049689441, "hmm_cov": 1.0, "evalue": 3.906120164311476e-09, "score": 32.40493392944336, "start": 110, "stop": 149 }, { "id": "PF08220.18", "name": "HTH_DeoR", "length": 45, "aa_cov": 0.2795031055900621, "hmm_cov": 1.0, "evalue": 1.316015661889516e-06, "score": 24.176462173461914, "start": 110, "stop": 154 } ], "seq_stats": { "molecular_weight": 18713.68890000001, "isoelectric_point": 9.579115486145017 }, "id": "OAIKOHHJFB_1266", "locus": "GCA000209735_01252" }, { "type": "cds", "sequence": "contig_1", "start": 696628, "stop": 697197, "strand": "-", "frame": 2, "gene": null, "product": "Schlafen AlbA-2 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFG5", "UniRef:UniRef90_C4XFG5" ], "nt": "ATGATTATGGGAATAAACGTTCAAAAAGGAGAAAAAGCTATTTATTACATAAAAAAATATGGTTGTTCTTCAAAAGGATGTTTAGTAAGAGTTGGGACAAGCACAAGAGAAATGTCAAATGAAGAAATAGAAGAAAGAAACAAAATTAGATTTTATGATGAGGACAACATAATAAATTATGTTAGTGATTTGGATGATTTGAAATTTTCAATATTAAAAATAAATTATGAACTAAATAATTTACATTTAGATGATAAAACATTTGAAAAAAATTTACATTTAAGAAATAAATTAGGTAGTTACAATAAGCTAGCTGAATTGCTAGCTAATGAAAATAAAATTGACTTGAAATATGTAAAATTTCAAGGAAATGATAAAACAGTCTTTTCAGAAATACAAAAATTCAGTAATAAAAGTATATTGGTTGGTTATTCACAAATTTTAGCAAGATTAGAAGTTGAAAATAAATGCTATGTAGATACTAGTTCAATGCAAAGAATTGAAAAGACTTTATTTGATTTTAATGTAGCTAAAGAGGCTTTATTAAATGCAATTGTACATAATGATTGA", "aa": "MIMGINVQKGEKAIYYIKKYGCSSKGCLVRVGTSTREMSNEEIEERNKIRFYDEDNIINYVSDLDDLKFSILKINYELNNLHLDDKTFEKNLHLRNKLGSYNKLAELLANENKIDLKYVKFQGNDKTVFSEIQKFSNKSILVGYSQILARLEVENKCYVDTSSMQRIEKTLFDFNVAKEALLNAIVHND", "aa_hexdigest": "fa3e48686e34c851a0f79465d26d48d3", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 696325, "stop": 697446, "observations": { "mutation": [ 696630, 697281 ], "stop": [ 696630, 697281 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFG5", "query_cov": 1.0, "subject_cov": 0.42567567567567566, "identity": 1.0, "score": 367.0, "evalue": 8.54e-125, "gene_start": 1, "gene_end": 189, "reference_start": 84, "reference_end": 272, "reference_sequence": "MKFVETNKIELKEKLNDNLIREIVGFLNTEGGTIYIGVKDNGEVIGIKDVNKAFTELNEIICDQIEPRAEKEVKSEIKFENNKMIMGINVQKGEKAIYYIKKYGCSSKGCLVRVGTSTREMSNEEIEERNKIRFYDEDNIINYVSDLDDLKFSILKINYELNNLHLDDKTFEKNLHLRNKLGSYNKLAELLANENKIDLKYVKFQGNDKTVFSEIQKFSNKSILVGYSQILARLEVENKCYVDTSSMQRIEKTLFDFNVAKEALLNAIVHNDWLSGVPTIQQFENRIEIMSYGGLPKGLKLEEFYQGISKPRNPKLMKIFIDLNLAEQTGHGVPLILKKYGKMAIEVEKNYIRITIPFNKELLQKNNNYEIKEIKDKLNKIENQIFELIQKNEEYTAYELSNELEVSKRTIERALASLKNKLILKRIGSRKTGKWSVLGIKPEN" }, "paralog": false, "identity": 0.9946380697050938, "score": 732.0, "evalue": 7.7e-271, "description": "Internal stop codon at 696630, 697281. Nonsense mutation around 696630, 697281.", "psc": { "uniref90_id": "UniRef90_C4XFG5", "product": "Schlafen AlbA-2 domain-containing protein", "uniref50_id": "UniRef50_C4XFG5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFG5", "UniRef:UniRef50_C4XFG5" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFG5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFG5" ], "product": "Schlafen AlbA-2 domain-containing protein" } }, "id": "OAIKOHHJFB_1267", "locus": "GCA000209735_01253" }, { "type": "cds", "sequence": "contig_1", "start": 697279, "stop": 697446, "strand": "-", "frame": 2, "gene": null, "product": "Schlafen AlbA-2 domain-containing protein", "db_xrefs": [ "RefSeq:WP_015511091.1", "SO:0001217", "UniParc:UPI0001A8C356", "UniRef:UniRef100_C4XFG5", "UniRef:UniRef50_C4XFG5", "UniRef:UniRef90_C4XFG5" ], "nt": "ATGAAATTTGTTGAAACAAATAAAATCGAATTAAAGGAAAAATTAAATGATAACTTAATTAGAGAAATTGTTGGCTTTTTAAACACAGAAGGCGGAACAATTTATATAGGTGTAAAAGATAATGGTGAAGTAATTGGCATAAAAGATGTAAATAAAGCTTTTACTTAA", "aa": "MKFVETNKIELKEKLNDNLIREIVGFLNTEGGTIYIGVKDNGEVIGIKDVNKAFT", "aa_hexdigest": "c4fd7bde90b352d5d50cc0510f8c447e", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001A8C356", "ncbi_nrp_id": "WP_015511091.1", "uniref100_id": "UniRef100_C4XFG5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C356", "RefSeq:WP_015511091.1", "UniRef:UniRef100_C4XFG5" ] }, "ips": { "uniref100_id": "UniRef100_C4XFG5", "uniref90_id": "UniRef90_C4XFG5" }, "psc": { "uniref90_id": "UniRef90_C4XFG5", "product": "Schlafen AlbA-2 domain-containing protein", "uniref50_id": "UniRef50_C4XFG5" }, "pscc": { "uniref50_id": "UniRef50_C4XFG5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFG5" ], "product": "Schlafen AlbA-2 domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_1268", "locus": "GCA000209735_01254" }, { "type": "cds", "sequence": "contig_1", "start": 697571, "stop": 697708, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATATTAGATAAAAGAAGAGAAGATACACACCAAGAAGCACAAGAAATAATAGATGCAATTCAAGAAGACAATAGTCAAAATAATACTGTTGAAAATGATCCAAAAGAAGAACAAGAAGACAATAAAAAAGAATAA", "aa": "MILDKRREDTHQEAQEIIDAIQEDNSQNNTVENDPKEEQEDNKKE", "aa_hexdigest": "81dcc8efedbbd55ba062378f701c432f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5368.595700000001, "isoelectric_point": 4.316603279113769 }, "id": "OAIKOHHJFB_1269", "locus": "GCA000209735_01255" }, { "type": "cds", "sequence": "contig_1", "start": 698370, "stop": 699011, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08264.19" ], "nt": "ATGCTCGGTAATTTAAATGGTTTTAAATATGACTCAAATCTCAAAAGAATTGGCGTACATGCCTATATTAAAGAGCAATTGGAAAAAGTTAAAGCTCAAGTTATTGAAGCTTATGATGAATATAAATTCATTAACGTTATTAAACTTTTAAATAATTATGTAGTTGAATTATCAAGCTTTTATTTAAACATTTCAAAAGATGTTTTATATGTCGAAGCTAAAGATTCAATTAACCGTCTTATGACTTTAACAAATATTTATGAAATTGTTGATTTTCTAATTATTGCTCTTGCTCCAATTCTTCCAACTACAGCTGAAGATGCTTACAAAAACTTTAATAAAGTAAATAAAGCTGAAAGTGTTCATCTCGAAACTTTAGCTAACCCACTCAAAGTTAACGAACAAGTTTTAACTCAATGGCAAGACTTTTTTAAACTTCGTGATGAAGTAAACTTATCACTTGAAAATGCAATTAAAGAAGGTTTAATTAAAAGAACTAATGAAGCTAAAGTTGTAATTAAAAGCGATAGCGACTTTATTAAAAGTCTTGATTTAAGACAATTATTAATGGTTGGTAAAGTTGAATTTGGAGCTCAAACTTCTGTTTCAGTTTTCAAATCAATTAAATGCCAAAGATGTTGA", "aa": "MLGNLNGFKYDSNLKRIGVHAYIKEQLEKVKAQVIEAYDEYKFINVIKLLNNYVVELSSFYLNISKDVLYVEAKDSINRLMTLTNIYEIVDFLIIALAPILPTTAEDAYKNFNKVNKAESVHLETLANPLKVNEQVLTQWQDFFKLRDEVNLSLENAIKEGLIKRTNEAKVVIKSDSDFIKSLDLRQLLMVGKVEFGAQTSVSVFKSIKCQRC", "aa_hexdigest": "7d34ec40ca24057487bbf102d1303a56", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08264.19", "name": "Anticodon_1", "length": 154, "aa_cov": 0.7136150234741784, "hmm_cov": 0.948051948051948, "evalue": 2.4236194887697427e-23, "score": 78.73269653320312, "start": 22, "stop": 173 } ], "seq_stats": { "molecular_weight": 24419.04140000001, "isoelectric_point": 7.664419746398925 }, "id": "OAIKOHHJFB_1270", "locus": "GCA000209735_01256" }, { "type": "cds", "sequence": "contig_1", "start": 699103, "stop": 699258, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTACTCAAAGACGTAAAATATCAGCTCCATTTTTGCTAATTATTTTAAGAGGATCAACAACATTACCTTTTGATTTAGACATTTTTTCACCTTTAGCATCTAAAACAAAACCGTGTGAAACAATGTTTTTGTATGGTGCTTTACCATTATAA", "aa": "MATQRRKISAPFLLIILRGSTTLPFDLDIFSPLASKTKPCETMFLYGALPL", "aa_hexdigest": "a57b935c98712aa1a5d40de0d71e357f", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5684.778299999999, "isoelectric_point": 9.622825050354006 }, "id": "OAIKOHHJFB_1271", "locus": "GCA000209735_01257" }, { "type": "cds", "sequence": "contig_1", "start": 699762, "stop": 700070, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432F9R0" ], "nt": "ATGCAATATGTAAGCCCACTTAATAAAAATGTATGTCCTGTAGTTATAGGCCACCACGTTACTTTAGAAAGTGGTAGTGGTTTAGTTCATATTGCTCCTCTTTTTGGTGAAGATGACTTCCAAATTGGAATCAAAAACTCACTTAAAATGATTATGCATATTGAAGATGATGGAAAAATTAATAAAGAAGGTGGAGCTTACCAAGGTCAATTTTATGAAAAAGCTAATGCTTTAATTATTGAAGAATTAACTAAATCTAAAAACTTACTTTTAGAAAGCTCAATAACTCACTCATATCCACATGACTGA", "aa": "MQYVSPLNKNVCPVVIGHHVTLESGSGLVHIAPLFGEDDFQIGIKNSLKMIMHIEDDGKINKEGGAYQGQFYEKANALIIEELTKSKNLLLESSITHSYPHD", "aa_hexdigest": "18fec76ff2f8107dfec1b0fcc070f139", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A432F9R0", "query_cov": 0.9901960784313726, "subject_cov": 0.9099099099099099, "identity": 0.545, "score": 114.0, "evalue": 8.22e-31, "valid": false, "uniref50_id": "UniRef50_A0A432F9R0" }, "pscc": { "uniref50_id": "UniRef50_A0A432F9R0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432F9R0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11306.75819999999, "isoelectric_point": 5.5876367568969725 }, "id": "OAIKOHHJFB_1272", "locus": "GCA000209735_01258" }, { "type": "cds", "sequence": "contig_1", "start": 700401, "stop": 700646, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGCTTGAGGAAGCACACTTAAAGAAAAATGAATTAAGTGTTGTTGACTTACGTAAAAAAGCTGCTCTTTATGCTCAAGATCAAGTTAATAAACAAGCTGAACAATTCAAAAGCATGCAACTTTTATGTGACTTTAAAAAATACTACATTACTAAAGATCCTAAATTCGAAGCTCAACAATTAAGACTTTTTAAAAAGATGATTTTTAGTGGTCTTATTTACAAAGGATTAAAACCTGTTTATTGA", "aa": "MLEEAHLKKNELSVVDLRKKAALYAQDQVNKQAEQFKSMQLLCDFKKYYITKDPKFEAQQLRLFKKMIFSGLIYKGLKPVY", "aa_hexdigest": "7ce3774468e2a5fef7a2aaf7e5f48aaa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 74, "aa_cov": 0.9135802469135802, "hmm_cov": 1.0, "evalue": 2.135302695026712e-11, "score": 39.24574279785156, "start": 8, "stop": 81 } ], "seq_stats": { "molecular_weight": 9619.299899999995, "isoelectric_point": 9.576665687561036 }, "id": "OAIKOHHJFB_1273", "locus": "GCA000209735_01259" }, { "type": "cds", "sequence": "contig_1", "start": 700866, "stop": 700964, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAATTGATTACAAAAAAACATTAAATATGCCTCAAACTAATTTCGATATGCGTGCTAACTTAACAACTAAAGAGCCAGCATTCCGAAAAGAATGA", "aa": "MAIDYKKTLNMPQTNFDMRANLTTKEPAFRKE", "aa_hexdigest": "77547607ab02993b7910716ce7b0018c", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3804.3769999999995, "isoelectric_point": 9.397443580627442 }, "id": "OAIKOHHJFB_1274", "locus": "GCA000209735_01260" }, { "type": "cds", "sequence": "contig_1", "start": 700976, "stop": 701743, "strand": "-", "frame": 1, "gene": null, "product": "CTP synthase", "db_xrefs": [ "EC:6.3.4.2", "GO:0003883", "GO:0006221", "GO:0006241", "KEGG:K01937", "SO:0001217", "UniRef:UniRef50_Q98PQ3", "UniRef:UniRef90_C4XFG8" ], "nt": "TTGAATAAATATCACGCAACTAAAAATTATAAAATTAACTTACTTTTAGTTGGTAAATATACTGAATTAGAAGATGCTTATTTATCAATTATTTCTTCATTAAAAATAGCTGCTGTTCATCAAAATGTCGAATTAAAATATGACTTAATTGATGCTGATTTAGTTAACAATAAAAACATTGAAAAATTAACAAAAGCACATGATGCAGTAATGATTTTACCAGGCTTTGGTGCTAGAGGTTTTGAATCTAAAGTTAATGTTGCGACTTACACAAGAGAACACAAAATTCCTACTTTAGGTATTTGTTTAGGTTTTCAAGCAATGTCAGTAGCTCAAGCTCGATTATTAGGCATTAAAGATGCAACCAGCAAAGAATTCGCTGAAAATGGTAAAAAACAAACTTTTGTTTTAACTCCTTTTTATGAAAATGGAGACAAAATGGCTATTGGTGGCACACTAAGACTTGGTGAAGATCAAGTTCAAGCTAAAAATAACACCTTAGCTCAAAAAATTTATGGTTCAAATATTTTCTATGAACGTCACAGACATAGATATGAAATTAATCCTGAATACTTAGAAAAATTACAAGATGATGAATTTACATTCAGTGCAATTCATCCAACTAAAAAAGTGGCTGAAATTTGTGAAGTTAAAAACCATCCTTTCTATTTAGGTGTTCAATATCACCCAGAATTTAGTACTAGAGTTTTAGCTTCAAATCCACTTTTTGATAATTTCTTCCAAGCAATTATTAAAAATCAAAAATAG", "aa": "MNKYHATKNYKINLLLVGKYTELEDAYLSIISSLKIAAVHQNVELKYDLIDADLVNNKNIEKLTKAHDAVMILPGFGARGFESKVNVATYTREHKIPTLGICLGFQAMSVAQARLLGIKDATSKEFAENGKKQTFVLTPFYENGDKMAIGGTLRLGEDQVQAKNNTLAQKIYGSNIFYERHRHRYEINPEYLEKLQDDEFTFSAIHPTKKVAEICEVKNHPFYLGVQYHPEFSTRVLASNPLFDNFFQAIIKNQK", "aa_hexdigest": "dce53efa9165266ca3dd04c6823f5906", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 700976, "stop": 702043, "observations": { "mutation": [ 701755 ], "stop": [ 701755 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFG8", "query_cov": 1.0, "subject_cov": 0.46703296703296704, "identity": 0.996, "score": 512.0, "evalue": 4.45e-179, "gene_start": 1, "gene_end": 255, "reference_start": 292, "reference_end": 546, "reference_sequence": "MNPIVLRRIIVTKFIVTTGGVLSGLGKGVTSASIGNLLKAQGFKVFALKLDPYLNIDPGVMSPIEHGEVFVTADGGETDLDLGHYERFIDVKLSKESNYTSGRIFTRIFEKERQGLYGGKTVQIVPHVIDEIISIIENTAKKQKPDFMLIEIGGTVGDIESNPYIYAISKFASLNPKSVFFAHLAFIPYLTASKEYKSKPSQVSIASLRSFGINPNILLLRSQGEVDERIINKVAEASFLDPNHVIGVPDKSSIYEIPMFLYEQNILKIIYSHFGIKKQINDKVYDSWKVFLNKYHATKNYKINLLLVGKYTELEDAYLSIISSLKIAAVHQNVELKYDLIDADLVNNKNIEKLTKAHDAVMILPGFGARGFESKVNVATYTREHKIPTLGICLGFQAMSVAQARLLGIKDATSKEFAENGKKQTFVLTPFYENGDKMAIGGTLRLGEDQVQAKNNTLAQKIYGSNIFYERHRHRYEINPEYLEKLQDDEFTFSAIHPTKKVAEICEVKNHPFYLGVQYHPEFSTRVLASNPLFDNFFQAIIKNQK" }, "paralog": false, "identity": 0.9971830985915493, "score": 703.0, "evalue": 8.21e-257, "description": "Internal stop codon at 701755. Nonsense mutation around 701755.", "psc": { "uniref90_id": "UniRef90_C4XFG8", "product": "CTP synthase", "ec_ids": [ "6.3.4.2" ], "uniref50_id": "UniRef50_Q98PQ3", "kegg_orthology_id": "K01937", "go_ids": [ "GO:0003883", "GO:0006221", "GO:0006241" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFG8", "EC:6.3.4.2", "UniRef:UniRef50_Q98PQ3", "KEGG:K01937", "GO:0003883", "GO:0006221", "GO:0006241" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PQ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PQ3" ], "product": "CTP synthase" } }, "id": "OAIKOHHJFB_1275", "locus": "GCA000209735_01261" }, { "type": "cds", "sequence": "contig_1", "start": 701753, "stop": 702586, "strand": "-", "frame": 1, "gene": null, "product": "CTP synthase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223MAG4" ], "nt": "GTGACTAAATTTATTGTGACAACTGGTGGAGTTTTATCTGGTTTAGGTAAGGGTGTTACCAGTGCAAGTATTGGAAATTTATTAAAGGCACAAGGCTTTAAAGTCTTTGCTTTAAAACTTGATCCATATTTAAATATTGACCCAGGAGTAATGAGTCCCATTGAACATGGTGAAGTATTTGTAACTGCTGATGGTGGTGAGACTGACTTAGACTTAGGACACTATGAAAGATTTATTGATGTTAAGCTAAGCAAGGAAAGTAACTACACAAGTGGAAGAATTTTTACTCGCATTTTTGAAAAAGAACGTCAAGGACTTTATGGTGGAAAAACTGTTCAAATAGTACCTCATGTTATTGATGAAATTATTAGCATTATCGAAAATACTGCTAAAAAACAAAAACCAGATTTCATGTTAATTGAAATCGGAGGAACTGTTGGAGACATTGAAAGTAATCCTTATATTTATGCAATTAGTAAGTTTGCTAGTTTAAATCCTAAAAGTGTTTTCTTTGCTCACTTAGCATTTATTCCTTATTTAACTGCTTCAAAAGAATACAAATCAAAACCATCTCAAGTTTCAATTGCTTCTTTAAGATCATTTGGAATTAACCCTAATATTTTACTTTTAAGAAGTCAAGGCGAAGTTGATGAAAGAATTATTAATAAAGTTGCTGAAGCTTCATTTTTGGACCCTAATCATGTAATTGGAGTTCCTGATAAAAGCAGTATCTATGAAATTCCAATGTTCTTATATGAACAAAATATTTTAAAAATAATTTACTCACATTTTGGAATTAAAAAACAAATTAATGACAAGGTTTATGATTCATGA", "aa": "MTKFIVTTGGVLSGLGKGVTSASIGNLLKAQGFKVFALKLDPYLNIDPGVMSPIEHGEVFVTADGGETDLDLGHYERFIDVKLSKESNYTSGRIFTRIFEKERQGLYGGKTVQIVPHVIDEIISIIENTAKKQKPDFMLIEIGGTVGDIESNPYIYAISKFASLNPKSVFFAHLAFIPYLTASKEYKSKPSQVSIASLRSFGINPNILLLRSQGEVDERIINKVAEASFLDPNHVIGVPDKSSIYEIPMFLYEQNILKIIYSHFGIKKQINDKVYDS", "aa_hexdigest": "2c924df9d86857f0914b074129c2acee", "start_type": "GTG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI00387385AD", "query_cov": 0.9855595667870036, "subject_cov": 0.8694267515923567, "identity": 0.6809999999999999, "score": 385.0, "evalue": 2.63e-132, "valid": false, "product": "CTP synthase", "ec_ids": [ "6.3.4.2" ], "uniref50_id": "UniRef50_A0A223MAG4", "kegg_orthology_id": "K01937", "go_ids": [ "GO:0003883", "GO:0006221", "GO:0006241" ] }, "pscc": { "uniref50_id": "UniRef50_A0A223MAG4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223MAG4" ], "product": "CTP synthase" }, "genes": [], "id": "OAIKOHHJFB_1276", "locus": "GCA000209735_01262" }, { "type": "cds", "sequence": "contig_1", "start": 702891, "stop": 703814, "strand": "+", "frame": 3, "gene": null, "product": "S1 motif domain-containing protein", "db_xrefs": [ "KEGG:K06959", "SO:0001217", "UniRef:UniRef50_L0RUW1", "UniRef:UniRef90_C4XFG9" ], "nt": "ATGGATTTATCTAAAAAATTTGTAGCACAAAAATTAAACTTAAAAGAAGACCAAGTTGAAGTTGTTCTTAAGTTAATGGATGAAGGAGCAACAGTACCTTTTATTGCCCGTTATCGTAAAAATGTAACTGGTGGACTTGATGAAGAAGTTATTCAACAAATAAGTGACATGTATGTTTACAATGTTGAATTAAACAAAAGAAAAGAAGCTATTATTGACATCTTAAAAGAAAAAGGATTACTCACACCTGAAATTGAAAATAAATTAAAAGCAGCTGAAACTAAAGCTGAAGTTGAAAACATTTATGAACCATTTAAAGTTGGTAAAAAAACTAAAGCTAGTGAAGCTATTGCTTTAGGTTTAGAACCTTTAGCTAAAGCAATTATGGAAGCAACTGATGAAAGTTTTGATCCTTATAAAGAAGCTAAAAAATACTTAAATGACAAAGTACCAACAGTTGAATTTGCTATTGAGCAAGCTCAATACATTATTAGCCAAATTATGTCTCAAGATGTTGAAAATAGAGAATATGTTAAAAAACAAATTTATGATTTTGGTTTCATTGTAACAAAAGTTAAAAAGAATGCTGAAGATGAAAAAGAAGTATTTCAACAATACTATGATTATAAAGAAAGAATCAAGTTTATTCCTAACCATCGTGTTTTAGCAATTAGTCGTGGTGAAGACAAAAAGATTTTAAGTTATGACATGACTTATAATTTAGCTAAAATTACTTATGACTTAAACAACAAATATTTTAAAATCAAAAGAACAGGCAAGATTTTGAACGAATCATTAAAAGACAGTTTAGATCGTTTGATTTTACCTTCAATTATTAGAGAAATCAAAAGTGATCTTTTTGCTCGTGCAGAAAAAGAAGCAATTAACCTTTTCGCAACTAATCTTGAAACAATGTTACTATGA", "aa": "MDLSKKFVAQKLNLKEDQVEVVLKLMDEGATVPFIARYRKNVTGGLDEEVIQQISDMYVYNVELNKRKEAIIDILKEKGLLTPEIENKLKAAETKAEVENIYEPFKVGKKTKASEAIALGLEPLAKAIMEATDESFDPYKEAKKYLNDKVPTVEFAIEQAQYIISQIMSQDVENREYVKKQIYDFGFIVTKVKKNAEDEKEVFQQYYDYKERIKFIPNHRVLAISRGEDKKILSYDMTYNLAKITYDLNNKYFKIKRTGKILNESLKDSLDRLILPSIIREIKSDLFARAEKEAINLFATNLETMLL", "aa_hexdigest": "f3778c4d57722586d524453307c6b7d7", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 702879, "stop": 704117, "observations": { "mutation": [ 703812 ], "stop": [ 703812 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFG9", "query_cov": 1.0, "subject_cov": 0.425207756232687, "identity": 1.0, "score": 578.0, "evalue": 7e-202, "gene_start": 1, "gene_end": 307, "reference_start": 5, "reference_end": 311, "reference_sequence": "MEENMDLSKKFVAQKLNLKEDQVEVVLKLMDEGATVPFIARYRKNVTGGLDEEVIQQISDMYVYNVELNKRKEAIIDILKEKGLLTPEIENKLKAAETKAEVENIYEPFKVGKKTKASEAIALGLEPLAKAIMEATDESFDPYKEAKKYLNDKVPTVEFAIEQAQYIISQIMSQDVENREYVKKQIYDFGFIVTKVKKNAEDEKEVFQQYYDYKERIKFIPNHRVLAISRGEDKKILSYDMTYNLAKITYDLNNKYFKIKRTGKILNESLKDSLDRLILPSIIREIKSDLFARAEKEAINLFATNLETMLLWPAVKNKTVMAIDPAFVNGCKIAILDPQGKFLKKALIYPNPPQNYVDKAKEIVNKLLDSYKVNIIVIGNGTASRETEEFIANLLKERSKVNPNEDISYAVVSEVGASVYSASKVAIDEFPDFSVEERSAVNIGRRFQDPLNELVKIDPKAIGVGQYQHDVNQKDLSKALDFKVDKVVNMVGVDLNSATKEILCYISGLTKTLAQNIIDYREKINKFKERKELLKVKGLGEKAYEQAVGFLRIHDSKNFYDRTAIHPESYPLAINIVNALQLDLNDIDLKTIEQQDANKLAKDFNSNEYDVKLILDSLSNPTKDIRDDKDGYILKKDVLKLEDLKPGMILDGSVQNITDFGIFVYIGIKQAALIHISNMKRNKDHYIKHSSEVVKTGDNIKIQILEIDKERERIQGKLIWED" }, "paralog": false, "identity": 0.9951456310679612, "score": 795.0, "evalue": 2.27e-289, "description": "Internal stop codon at 703812. Nonsense mutation around 703812.", "psc": { "uniref90_id": "UniRef90_C4XFG9", "product": "S1 motif domain-containing protein", "uniref50_id": "UniRef50_L0RUW1", "kegg_orthology_id": "K06959", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFG9", "UniRef:UniRef50_L0RUW1", "KEGG:K06959" ] }, "pscc": { "uniref50_id": "UniRef50_L0RUW1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RUW1" ], "product": "S1 motif domain-containing protein" } }, "id": "OAIKOHHJFB_1277", "locus": "GCA000209735_01263" }, { "type": "cds", "sequence": "contig_1", "start": 703839, "stop": 705038, "strand": "+", "frame": 3, "gene": null, "product": "RNA (S1 domain)-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PA84" ], "nt": "ATGGCAATAGACCCTGCTTTTGTTAATGGTTGCAAAATTGCAATCTTAGATCCTCAAGGTAAGTTCTTAAAAAAAGCTTTAATTTATCCTAACCCTCCTCAAAATTATGTTGATAAAGCTAAAGAAATAGTTAATAAACTATTAGATTCATACAAAGTTAATATTATTGTTATTGGCAATGGAACAGCTTCAAGAGAAACTGAAGAATTTATTGCTAATTTATTAAAAGAAAGATCAAAAGTAAATCCTAATGAAGATATTTCTTATGCTGTTGTTAGTGAAGTAGGAGCTAGTGTTTATTCAGCTTCTAAAGTTGCTATTGATGAATTTCCAGATTTTAGTGTTGAAGAAAGATCAGCTGTTAATATTGGACGTCGTTTCCAAGATCCTCTTAATGAATTAGTTAAAATTGATCCTAAAGCTATAGGGGTAGGTCAATATCAACATGATGTTAATCAAAAAGATTTATCTAAGGCTTTAGACTTTAAAGTTGATAAAGTAGTTAACATGGTTGGAGTTGACTTAAATAGTGCTACTAAAGAAATCTTATGCTACATTTCAGGTTTAACTAAAACATTAGCTCAAAACATTATTGATTATCGTGAAAAAATCAATAAATTTAAAGAAAGAAAAGAACTATTAAAGGTTAAAGGTTTAGGCGAAAAAGCCTATGAACAAGCGGTTGGCTTCTTAAGAATCCATGATTCTAAAAACTTTTATGACCGCACAGCTATTCACCCTGAATCATATCCTTTAGCAATTAATATTGTTAATGCGCTTCAATTAGATCTAAATGATATAGACTTAAAAACTATTGAACAACAAGATGCTAATAAATTAGCTAAAGACTTTAATTCAAATGAATATGATGTTAAGTTAATATTAGATTCTTTATCTAATCCAACTAAAGATATTCGTGATGACAAAGATGGCTACATTCTTAAAAAAGATGTTCTTAAGTTAGAAGACTTAAAACCAGGTATGATTCTAGATGGTTCAGTGCAAAATATTACAGACTTTGGTATCTTTGTTTATATTGGAATTAAGCAAGCTGCTTTAATTCACATTTCAAATATGAAAAGAAATAAAGATCACTACATCAAACACTCTAGCGAAGTAGTTAAAACAGGAGATAACATTAAAATCCAAATCCTTGAAATAGATAAAGAAAGAGAAAGAATTCAAGGCAAATTAATTTGA", "aa": "MAIDPAFVNGCKIAILDPQGKFLKKALIYPNPPQNYVDKAKEIVNKLLDSYKVNIIVIGNGTASRETEEFIANLLKERSKVNPNEDISYAVVSEVGASVYSASKVAIDEFPDFSVEERSAVNIGRRFQDPLNELVKIDPKAIGVGQYQHDVNQKDLSKALDFKVDKVVNMVGVDLNSATKEILCYISGLTKTLAQNIIDYREKINKFKERKELLKVKGLGEKAYEQAVGFLRIHDSKNFYDRTAIHPESYPLAINIVNALQLDLNDIDLKTIEQQDANKLAKDFNSNEYDVKLILDSLSNPTKDIRDDKDGYILKKDVLKLEDLKPGMILDGSVQNITDFGIFVYIGIKQAALIHISNMKRNKDHYIKHSSEVVKTGDNIKIQILEIDKERERIQGKLI", "aa_hexdigest": "26d8279fcec8ca17602fadcc6d320034", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PA84", "query_cov": 1.0, "subject_cov": 0.9322429906542056, "identity": 0.541, "score": 412.0, "evalue": 3.05e-139, "valid": false, "product": "RNA (S1 domain)-binding protein", "uniref50_id": "UniRef50_A0A3B0PA84", "kegg_orthology_id": "K06959" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PA84", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PA84" ], "product": "RNA (S1 domain)-binding protein" }, "genes": [], "id": "OAIKOHHJFB_1278", "locus": "GCA000209735_01264" }, { "type": "cds", "sequence": "contig_1", "start": 705384, "stop": 705713, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013527071.1", "SO:0001217", "UniParc:UPI0001F59884", "UniRef:UniRef100_A0AB32XCI0", "UniRef:UniRef50_A0AB32XCI0", "UniRef:UniRef90_A0AB32XCI0" ], "nt": "GTGCTTATTGATAAGGATGGTAGTATTTATATCGACATTTTAGGAATTGAAAAATTTCCTACTAAAACATTGACTAGAGAAGAACTTAACGAATATCAACTTTTTGTACACAATTTAGGTGTGACAGAAAGCACAATATCAAAACTTCTTTATACAAAAATCAAATCAACAAATAGTTTTTATAACTTAATAGAATCTAAAATTGAAAAATTAAAAAATCTTTTACTCACTCTAAATTTAGACAAAAATAAATTAGAAAGCGAAATAAAAGATTTTACTCATAACATCTGTAAGATCAATAAAAAAATAGGTGAATTAATTCAATTCTAA", "aa": "MLIDKDGSIYIDILGIEKFPTKTLTREELNEYQLFVHNLGVTESTISKLLYTKIKSTNSFYNLIESKIEKLKNLLLTLNLDKNKLESEIKDFTHNICKINKKIGELIQF", "aa_hexdigest": "4baa41c4450eac7e5c49f59b799df526", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59884", "ncbi_nrp_id": "WP_013527071.1", "uniref100_id": "UniRef100_A0AB32XCI0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59884", "RefSeq:WP_013527071.1", "UniRef:UniRef100_A0AB32XCI0" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCI0", "uniref90_id": "UniRef90_A0AB32XCI0" }, "psc": { "uniref90_id": "UniRef90_A0AB32XCI0", "uniref50_id": "UniRef50_A0AB32XCI0" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XCI0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCI0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12694.667799999981, "isoelectric_point": 7.791342735290527 }, "id": "OAIKOHHJFB_1279", "locus": "GCA000209735_01265" }, { "type": "cds", "sequence": "contig_1", "start": 706514, "stop": 706783, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGAATTAGAACTATCATTTAAATATACTGATGAGCTTGTCAATTATCTTCTAAAAATTGAAAAATACAAAAGTTCATTAGAATTTTTGTCTTTACCTACAAGAAGTAAACAAAGAATTCAATTTGAAGCCAAAATTAAACAAACTCATTTTTCAACAAGTATTGAAGGCAATGTTTTAAACATTGAACAAGTTAAAAACGTTATAAACAATAAAACACATCAAACTAGAATTAAAGCTGAACAAGAAGTATTAAATTATTGA", "aa": "MKKELELSFKYTDELVNYLLKIEKYKSSLEFLSLPTRSKQRIQFEAKIKQTHFSTSIEGNVLNIEQVKNVINNKTHQTRIKAEQEVLNY", "aa_hexdigest": "3d82860e9b0c0ebfc8c324f315e3afd0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10577.044399999992, "isoelectric_point": 9.362888526916507 }, "id": "OAIKOHHJFB_1280", "locus": "GCA000209735_01266" }, { "type": "cds", "sequence": "contig_1", "start": 707150, "stop": 707296, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGATGATTATGATTTCAAAGGATTTAATTCTTTTGAAGAATATTATAGTAATAACTTAAATGAATATTATCAAGCATTACAAATGGATTTAAGTCCTTTATTTTATAATGGACGTAATAAACTGCCACATTTAGAAAAATGA", "aa": "MLDDYDFKGFNSFEEYYSNNLNEYYQALQMDLSPLFYNGRNKLPHLEK", "aa_hexdigest": "8860f11e0467944d43d5caa6eeffcec1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5876.4092, "isoelectric_point": 4.499567985534668 }, "id": "OAIKOHHJFB_1281", "locus": "GCA000209735_01267" }, { "type": "cds", "sequence": "contig_1", "start": 707318, "stop": 707518, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTCTTAATGCACAAAATATATATTTAAGTGCTTTAGAAGCAAGCACTAAAGATAATAGTTATAATGATTTGTTAAATAATCTTAATAAAAAAGACTTAACTTTATTAAGATATTGCATAGAAAATAAAAAAAGTATTATTCTTATTAAAGATTTAGCTTTAGTCTTTGGCGTTACTTCTAGAGCTATATCAAAATGA", "aa": "MALNAQNIYLSALEASTKDNSYNDLLNNLNKKDLTLLRYCIENKKSIILIKDLALVFGVTSRAISK", "aa_hexdigest": "520df7bb3923570945a2a691df50f1fa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7402.5457, "isoelectric_point": 9.206681632995604 }, "id": "OAIKOHHJFB_1282", "locus": "GCA000209735_01268" }, { "type": "cds", "sequence": "contig_1", "start": 708211, "stop": 708393, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013527068.1", "SO:0001217", "UniParc:UPI0001A8C360", "UniRef:UniRef100_C4XFH5", "UniRef:UniRef50_C4XFH5", "UniRef:UniRef90_C4XFH5" ], "nt": "ATGTTATTAGTTATTTTAAAAGAAAACATCAATGACAAACAAAAATATTTAAAAGAATATTTAATAACATTCTTATTAAGTGATATATTAGAACACTATAATGTCAAAAATGACTATAAAATAGAGGAATTATTAAAATTCTTAGCTTCTAGTTCTTCATCTTTAACTAATACTAAAAAATAA", "aa": "MLLVILKENINDKQKYLKEYLITFLLSDILEHYNVKNDYKIEELLKFLASSSSSLTNTKK", "aa_hexdigest": "af8ef68bb503186a0d73662924daf43a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C360", "ncbi_nrp_id": "WP_013527068.1", "uniref100_id": "UniRef100_C4XFH5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C360", "RefSeq:WP_013527068.1", "UniRef:UniRef100_C4XFH5" ] }, "ips": { "uniref100_id": "UniRef100_C4XFH5", "uniref90_id": "UniRef90_C4XFH5" }, "psc": { "uniref90_id": "UniRef90_C4XFH5", "uniref50_id": "UniRef50_C4XFH5" }, "pscc": { "uniref50_id": "UniRef50_C4XFH5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFH5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7095.2107, "isoelectric_point": 7.947291755676269 }, "id": "OAIKOHHJFB_1283", "locus": "GCA000209735_01269" }, { "type": "gap", "sequence": "contig_1", "start": 708932, "stop": 709031, "strand": ".", "length": 100, "id": "OAIKOHHJFB_1810" }, { "type": "cds", "sequence": "contig_1", "start": 710662, "stop": 711024, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGGCCCATATCCCTACGATGGATATTATTTTGGTAAAAATGTTCCTGATGAAGAAATAGAAAAAAATATCGAAATTCTATATGGGCTAATAGATTTATATGACAAAATAAATAAAATTGACTTTCTTCAAGACGAATCAGTAAACAAAGATAGTTATTCAAATTTAAAACAATTAAGAAAATATAAAAATCATTTTGATTCAAACTTTGCTTCAAATCCTTTTGGTTCATTTTATCTAAAATATAAAAATAATAACTCATCTGTATTAGCAGAAGCTATTAAGACTCAATATTTAACTATTAATGCAAATTCAAATATTTTAATAAGAGAAATAAAACAAAATTCAATAGGAAAATGA", "aa": "MFGPYPYDGYYFGKNVPDEEIEKNIEILYGLIDLYDKINKIDFLQDESVNKDSYSNLKQLRKYKNHFDSNFASNPFGSFYLKYKNNNSSVLAEAIKTQYLTINANSNILIREIKQNSIGK", "aa_hexdigest": "d36c5471216c6bac02d91de7b169b522", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14013.55789999998, "isoelectric_point": 6.572372245788575 }, "id": "OAIKOHHJFB_1284", "locus": "GCA000209735_01270" }, { "type": "cds", "sequence": "contig_1", "start": 711049, "stop": 711165, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAATATCTAATTCAGCTCCTGGGAAAGAAATCAAATTAACTAAAAATGGTTTTTATTTAATATATGACATATCACTAGATAAAACAAATATTAATAAATTTAAAATTGTTTGA", "aa": "MEISNSAPGKEIKLTKNGFYLIYDISLDKTNINKFKIV", "aa_hexdigest": "4e8472f64594401e7d09d6fa39250277", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4347.0371000000005, "isoelectric_point": 9.158330345153807 }, "id": "OAIKOHHJFB_1285", "locus": "GCA000209735_01271" }, { "type": "cds", "sequence": "contig_1", "start": 711201, "stop": 711437, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATTTCTGGTTTGATTTCAAAATTTTCTTCAAAAGATTTTTCAATTAAAAAATTATTATACTCATTAAAAATATCATCATATTTTAATTTATCAAGATCTTTTTTTGTTATGTTTAAATCAATAATTTTTTTCTTAGCTAAGTATTTTTCGAAATCATTTGAAACTTTAAGATTTTTATTTCTTCAAAATTTCTTGATAAATGATGGAGAATTTGCTTCTTCTGTTAAATAA", "aa": "MLISGLISKFSSKDFSIKKLLYSLKISSYFNLSRSFFVMFKSIIFFLAKYFSKSFETLRFLFLQNFLINDGEFASSVK", "aa_hexdigest": "155e05d570100d73304fda214788c006", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9153.74259999999, "isoelectric_point": 9.997321891784669 }, "id": "OAIKOHHJFB_1286", "locus": "GCA000209735_01272" }, { "type": "cds", "sequence": "contig_1", "start": 711778, "stop": 712188, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAAATCTGCTAAAGATATAAATAACAAAGACATTGACTTGAATATTATCGAATTAAAACTTTTGAAAGATAGTGATGAAAATCAAATAATAGTTAAATCTATATCAAAAGACAAAAACTATTATATAGCTAATAATGATACATATACATACAATGTAAGTTTACTTAAATCAGATTATAGTTATTTATTGAATTTTAATCCTGGAACTTTAAATGTTTTTGGTTTAAAAGATAAAGAAGAAATCAAAAAATACATTAAAGAACTATTAAAGAATTATTTAATCAAATACAATGAAAAAATTGATCAATCTCTTTACAAAAAAATCACAATAGATGATATTAATATATCGTTCAATGATGAAGAGTTAAACAAAATAATTAATCCAAAAGACGATGACATTTATTGA", "aa": "MIKSAKDINNKDIDLNIIELKLLKDSDENQIIVKSISKDKNYYIANNDTYTYNVSLLKSDYSYLLNFNPGTLNVFGLKDKEEIKKYIKELLKNYLIKYNEKIDQSLYKKITIDDINISFNDEELNKIINPKDDDIY", "aa_hexdigest": "b4c53e52ea2b991297e5b2d952de8beb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16044.07829999999, "isoelectric_point": 5.063240242004394 }, "id": "OAIKOHHJFB_1287", "locus": "GCA000209735_01273" }, { "type": "cds", "sequence": "contig_1", "start": 712339, "stop": 712776, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAATAAAAAAGACCCTGTTAAAATGGCTCAAATTAAAAATGAGGCTATTAGACAAGTTAGAGAAAAAATAATAGAAGAATTTAAAAAGAGAGCTCCAAAGCTTGAAATTGATAAGTTATATACTATTGATTTTACTGATTCATTTTTCAATAATTTAGATTTGTCTATCGGAACAACCTTAACTTTAAAAGGCAAGCATTTTATGTTGAAAAATCCTTATACAGTTAATGTTATAAATACTCATTCATCAGCAAACAAAAAATTAAATTTAAAAGATTTAAATTTTGAAGTTATTACTTTAAGTGAAAAGAATTTAAATGATTTCAAAAAAGCTTTAAATGAAAAAATTATTGATATTCTCAAAAAAGAAAATTATCCTGCTAAAGAAATAATCAAATTAATTGAATTTAAATTATTAGAAAACCAAAAGTGA", "aa": "MENKKDPVKMAQIKNEAIRQVREKIIEEFKKRAPKLEIDKLYTIDFTDSFFNNLDLSIGTTLTLKGKHFMLKNPYTVNVINTHSSANKKLNLKDLNFEVITLSEKNLNDFKKALNEKIIDILKKENYPAKEIIKLIEFKLLENQK", "aa_hexdigest": "68bbb28bf58d56b7fd3ef29e1a1a764c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17010.710999999992, "isoelectric_point": 9.435415458679199 }, "id": "OAIKOHHJFB_1288", "locus": "GCA000209735_01274" }, { "type": "cds", "sequence": "contig_1", "start": 712816, "stop": 713013, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGATTCAATTAAAAGAAGAATCGAATTTAATAAGTGGAGATAAAGAATTTAAATTAATTAATATTTTTACAGAAGAAATGTTAAAAGAAGCTTTAGAAATTAAATCTAAAAACAAGAAAAAATTACTTTCATGGCTAATTCCAGTAATTATTTTAAGTGTTGCTGGAATTATTGCAACAGTTATTGCAATATGA", "aa": "MMIQLKEESNLISGDKEFKLINIFTEEMLKEALEIKSKNKKKLLSWLIPVIILSVAGIIATVIAI", "aa_hexdigest": "f14970bb7a2b7d51169f76b839fd9002", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7341.860199999997, "isoelectric_point": 8.009826087951662 }, "id": "OAIKOHHJFB_1289", "locus": "GCA000209735_01275" }, { "type": "cds", "sequence": "contig_1", "start": 713116, "stop": 713862, "strand": "+", "frame": 1, "gene": null, "product": "Mbov_0401 family ICE element transposase-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFH7", "UniRef:UniRef90_C4XFH7" ], "nt": "ATGAATTTACAAGATAAGCAAAATTATTATGAAAGTAATTTGAATTTTTCATTAAGTTCATTAATTATGAATGATGAAAATTCATTAAATAAATTCATTGCTATTAACTTAATTAAAAATATGATTAACCAATTAATTAATTATAAATATAGTGATGAAAGAAAAAGTAAATATAAAGCTCATGATGTGATTATAAGAATTGTGAGATTTGAAAATTTTACATTTAAATTGAAAGTAGAAAGATATAAAGATAAAACAACAAATAAGTTGCATACATTTTATCCTGATTATTTAAATTTTGGATCATCTATATGTGAAGATGAAAAAGAAAGAATTCAAAATTATGCTGTTAAAGATAGAATCACTTATTCCAGAATTGCGAGCAAATACAATGTAAGCAAAACTCAAATATATAAAATTGTAAAACAAATAAATTTAGATAAAATAAACAGTCTAAAAAACTACAAGAATGAATTAGTAAACAATAACATTTTTATAGGTATCGATGATACATTTATAAATTCAAATCAAGGAAGAACAAAACAAAAACTAAAAATAAGATTTTTAACCATATATCAATTGAAGAATAATAAAATAGTTAATTTGAATAAAATAATGAATATTTTGCCATCTTCTAAAATGATTTCTGGCAAATGTTTATCAAAGACAATTAAAGAAGCAATATCAAAAAATTATGGAGATTATAATAGCTTTAATGTTTATATTTTAGGGGATGGTGCTCATTGA", "aa": "MNLQDKQNYYESNLNFSLSSLIMNDENSLNKFIAINLIKNMINQLINYKYSDERKSKYKAHDVIIRIVRFENFTFKLKVERYKDKTTNKLHTFYPDYLNFGSSICEDEKERIQNYAVKDRITYSRIASKYNVSKTQIYKIVKQINLDKINSLKNYKNELVNNNIFIGIDDTFINSNQGRTKQKLKIRFLTIYQLKNNKIVNLNKIMNILPSSKMISGKCLSKTIKEAISKNYGDYNSFNVYILGDGAH", "aa_hexdigest": "c3fcc899803f7ec5ead3347237f6e376", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 713041, "stop": 714165, "observations": { "mutation": [ 713860, 714004 ], "stop": [ 713860, 714004 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFH7", "query_cov": 1.0, "subject_cov": 0.5767441860465117, "identity": 1.0, "score": 484.0, "evalue": 3.89e-170, "gene_start": 1, "gene_end": 248, "reference_start": 26, "reference_end": 273, "reference_sequence": "MKPIGFFFYFCDFTKNTIKQAYNKNMNLQDKQNYYESNLNFSLSSLIMNDENSLNKFIAINLIKNMINQLINYKYSDERKSKYKAHDVIIRIVRFENFTFKLKVERYKDKTTNKLHTFYPDYLNFGSSICEDEKERIQNYAVKDRITYSRIASKYNVSKTQIYKIVKQINLDKINSLKNYKNELVNNNIFIGIDDTFINSNQGRTKQKLKIRFLTIYQLKNNKIVNLNKIMNILPSSKMISGKCLSKTIKEAISKNYGDYNSFNVYILGDGAHWIQTLAKQLKGNFILCKFHFLRKFNDFIFKKRNRGFNNFIDSIQNLSGWKIEKEISEAYNSRDYGKLLDILNTIYLYFRDSFVLSDIDRYNLNSLIRYTSKLESGIINNDYSSLIEGHISVYIKRKLNKSFALYSQKTIEALLLNDNKIQNTLIQTY" }, "paralog": false, "identity": 0.9919786096256684, "score": 742.0, "evalue": 5.25e-274, "description": "Internal stop codon at 713860, 714004. Nonsense mutation around 713860, 714004.", "psc": { "uniref90_id": "UniRef90_C4XFH7", "product": "Mbov_0401 family ICE element transposase-like protein", "uniref50_id": "UniRef50_C4XFH7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFH7", "UniRef:UniRef50_C4XFH7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFH7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFH7" ], "product": "Transposase" } }, "id": "OAIKOHHJFB_1290", "locus": "GCA000209735_01276" }, { "type": "cds", "sequence": "contig_1", "start": 714058, "stop": 714333, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH8", "UniRef:UniRef90_C4XEH8" ], "nt": "TTGTTAGATATATTAAATACAATTTATCTCTATTTTAGAGACTCTTTTGTTTTGAGCGATATTGATAGATACAATTTAAATAGTTTGATAAGATACACATCTAAACTTGAAAGTGGAATCATTAATAATGATTATAGTTCACTAATAGAAGGACACATATCTGTTTATATTAAAAGAAAACTAAACAAAAGCTTTGCATTATATTCTCAAAAAACAATAGAAGCTTTATTGTTGAATGATAATAAAATTCAAAATACTCTTATTCAGACATATTAG", "aa": "MLDILNTIYLYFRDSFVLSDIDRYNLNSLIRYTSKLESGIINNDYSSLIEGHISVYIKRKLNKSFALYSQKTIEALLLNDNKIQNTLIQTY", "aa_hexdigest": "25f9e36794dd6b75a6d134d610038f68", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_C4XEH8", "query_cov": 1.0, "subject_cov": 0.8053097345132744, "identity": 0.9890000000000001, "score": 174.0, "evalue": 4.75e-55, "valid": true, "uniref50_id": "UniRef50_C4XEH8" }, "pscc": { "uniref50_id": "UniRef50_C4XEH8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH8" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10689.122099999988, "isoelectric_point": 7.929176139831544 }, "id": "OAIKOHHJFB_1291", "locus": "GCA000209735_01277" }, { "type": "cds", "sequence": "contig_1", "start": 714674, "stop": 715120, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTTATACCTAGAAATTTGGAATATCGCATGTCGATATTCCTTTTTAATTATTAATATTGTTATTATCTTTTGTCCGCTAGGACAAATAAAAGTGAGGGAATTAACCCTCCCTAAAATATTTGGCAACTTGTCGCTCTTTCTAAAATATTATTTTAATTTTCCTATTTTTTTGAAAAACAAATTTCTATTCTTGTTACTTTCTCAGTTTCTCTTTTGTTTTCCATATAATAATTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCATACATTTCCAAAGCAGATAAATATCCAAAAATTTCTCTTGGACGATTGTTTATTTCATTTTGTACTTTTAGAATTTCTTCTTCAGTAATATCATCAAAATTAGTTTTTTTCTTAAAAAATCTTCTAACTAAACCATTGAAATTTTCATTTGAACCCCTTTGA", "aa": "MSLYLEIWNIACRYSFLIINIVIIFCPLGQIKVRELTLPKIFGNLSLFLKYYFNFPIFLKNKFLFLLLSQFLFCFPYNNLSIPVEFQGFSAFNFSYISKADKYPKISLGRLFISFCTFRISSSVISSKLVFFLKNLLTKPLKFSFEPL", "aa_hexdigest": "2f3c5b7ff591f6f7e321ae457d1cb3ae", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17417.73539999999, "isoelectric_point": 9.727328300476074 }, "id": "OAIKOHHJFB_1292", "locus": "GCA000209735_01278" }, { "type": "cds", "sequence": "contig_1", "start": 715171, "stop": 715284, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATTTTTAGATGTTTTTAATGAAACAGATATAAAAGCTCAAATATTAGAAATCAAAAATTATCAAGATATGTCATTTAACACTATAGAACAATTAAAAAATATAAAATAG", "aa": "MEFLDVFNETDIKAQILEIKNYQDMSFNTIEQLKNIK", "aa_hexdigest": "416431f6fb9cd22a558de94e679412d7", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4465.0621, "isoelectric_point": 4.440682792663575 }, "id": "OAIKOHHJFB_1293", "locus": "GCA000209735_01279" }, { "type": "cds", "sequence": "contig_1", "start": 715573, "stop": 715869, "strand": "-", "frame": 2, "gene": null, "product": "MobA-like NTP transferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFH9", "UniRef:UniRef90_C4XFH9" ], "nt": "ATGAAATTCGGAATTATACTATCTGCTGGTAATCAAACTAGATTTAAAAGCGCAACTCCTAAAGCCTTGGTTAATGTAAATGGAAAAATTTTACTTGAGGAAAATATTTTGCGAATGTCAAAATATTGTGATCATATTTTTACGATATGTTCTTCCCAAAATGAGCATTTTTTTGAAAAATATAATCATATTGCTATCTCATCAGGTAAGGGTAGTGGAGATGCTGTACTAAAAGCATTATTGGAAATCAAACAAAAATATGGTTTTTCTTCAAATGATACTTGTTTTATATTATGA", "aa": "MKFGIILSAGNQTRFKSATPKALVNVNGKILLEENILRMSKYCDHIFTICSSQNEHFFEKYNHIAISSGKGSGDAVLKALLEIKQKYGFSSNDTCFIL", "aa_hexdigest": "e22e22e646e501c11daa6d2383647b4f", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 715270, "stop": 715869, "observations": { "mutation": [ 715575 ], "stop": [ 715575 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFH9", "query_cov": 1.0, "subject_cov": 0.4224137931034483, "identity": 1.0, "score": 199.0, "evalue": 7.13e-63, "gene_start": 1, "gene_end": 98, "reference_start": 1, "reference_end": 98, "reference_sequence": "MKFGIILSAGNQTRFKSATPKALVNVNGKILLEENILRMSKYCDHIFTICSSQNEHFFEKYNHIAISSGKGSGDAVLKALLEIKQKYGFSSNDTCFILWGDCLIEDLIYKKVVENYKKVGIVPCVFEEKPYVQIFQKQDNKIDVLFSKFGENASSGFHDLSIFYFNCEEILKYLLDFQNKIIGRDGNYVHKHGNEMEFLDVFNETDIKAQILEIKNYQDMSFNTIEQLKNIK" }, "paralog": false, "identity": 0.9949748743718593, "score": 404.0, "evalue": 3.58e-146, "description": "Internal stop codon at 715575. Nonsense mutation around 715575.", "psc": { "uniref90_id": "UniRef90_C4XFH9", "product": "MobA-like NTP transferase domain-containing protein", "uniref50_id": "UniRef50_C4XFH9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFH9", "UniRef:UniRef50_C4XFH9" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFH9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFH9" ], "product": "MobA-like NTP transferase domain-containing protein" } }, "id": "OAIKOHHJFB_1294", "locus": "GCA000209735_01280" }, { "type": "cds", "sequence": "contig_1", "start": 715882, "stop": 716064, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAGAACCGGTCTTGACTTATCAAATGTAAAAATTTTACTTAATAAAAGTGCAAAAGAAACCTTAAAATTAACCATACCAAACTATAATTTCATAGTCAAAGATATGGGAAATATTAAGGGTAAACAAGAACCAGAAAAAATGGTTAAATCTGTTATTGATACCTATTATGATAAATAA", "aa": "MERTGLDLSNVKILLNKSAKETLKLTIPNYNFIVKDMGNIKGKQEPEKMVKSVIDTYYDK", "aa_hexdigest": "af346afa7d4768c0f60280ef41288d9a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6905.020500000004, "isoelectric_point": 9.260577201843262 }, "id": "OAIKOHHJFB_1295", "locus": "GCA000209735_01281" }, { "type": "cds", "sequence": "contig_1", "start": 716329, "stop": 716436, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAATTGATGATTTCGTTGTTTTAAATTCATTTGATAATATCTTTATCAAGGCATTAACGGAATCTGGAACAATAATAGATGATGAAGGAAAAACTGTCTTTTGA", "aa": "MGIDDFVVLNSFDNIFIKALTESGTIIDDEGKTVF", "aa_hexdigest": "2f130130cd1fc4a362479ed7b1caabdb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3865.3156, "isoelectric_point": 4.0500284194946286 }, "id": "OAIKOHHJFB_1296", "locus": "GCA000209735_01282" }, { "type": "cds", "sequence": "contig_1", "start": 716592, "stop": 716903, "strand": "-", "frame": 3, "gene": "cofD", "product": "2-phospho-L-lactate transferase CofD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI1", "UniRef:UniRef90_C4XFI1" ], "nt": "ATGAAAATAACTATTATTTCAGGTGGATCAGGTAATGATGCACTAGTAAAAGGATTAAAAGCTTTTTACAAAGATAAAAATTTAAGCATTAAAGTTATTGTAAATGCTTATGATAATGGAAAATCTACTGGTGTATGTAGAAAAGTTACTGATACTTTAGGAGTATCAGATATAAGAAAAAATCACTCAAGAATGTATGTGAGTGAATATGGCGATAAAGTGGATAAAAACCTTTTAGAGTTTTATGAAAAAAGATTTGATTTCACTAAAAATAAAGAAGAATTAGAAATTTCTAAACTTTTAGATAAATGA", "aa": "MKITIISGGSGNDALVKGLKAFYKDKNLSIKVIVNAYDNGKSTGVCRKVTDTLGVSDIRKNHSRMYVSEYGDKVDKNLLEFYEKRFDFTKNKEELEISKLLDK", "aa_hexdigest": "04bbf442d71f3436bf8ce0bed53fd935", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "cofD" ], "truncated": "5-prime", "pseudogene": { "start": 716409, "stop": 716903, "observations": { "mutation": [ 716594 ], "stop": [ 716594 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFI1", "query_cov": 1.0, "subject_cov": 0.6280487804878049, "identity": 1.0, "score": 202.0, "evalue": 3.01e-65, "gene_start": 1, "gene_end": 103, "reference_start": 1, "reference_end": 103, "reference_sequence": "MKITIISGGSGNDALVKGLKAFYKDKNLSIKVIVNAYDNGKSTGVCRKVTDTLGVSDIRKNHSRMYVSEYGDKVDKNLLEFYEKRFDFTKNKEELEISKLLDKWNLKEYKKYVSNFFKNPKANEFNYKDFSVSNIVYSQMYKELGYEKTNKHFAIKWELMISLF" }, "paralog": false, "identity": 0.9939024390243902, "score": 328.0, "evalue": 2.62e-117, "description": "Internal stop codon at 716594. Nonsense mutation around 716594.", "psc": { "uniref90_id": "UniRef90_C4XFI1", "product": "2-phospho-L-lactate transferase CofD", "uniref50_id": "UniRef50_C4XFI1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFI1", "UniRef:UniRef50_C4XFI1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFI1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI1" ] } }, "id": "OAIKOHHJFB_1297", "locus": "GCA000209735_01283" }, { "type": "cds", "sequence": "contig_1", "start": 717547, "stop": 718110, "strand": "-", "frame": 2, "gene": null, "product": "Glycosyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI2", "UniRef:UniRef90_C4XFI2" ], "nt": "GTGAAATTTAAGACTCAAACTCAAAAGCTAAAATATGGTATAGTCATTTGTGCTAGAAATGAAGAAAAAGTTATTGCTGAGTTAGTAAAAAGCATAAAAAATTCTCAATATGATCAAGATAAACTCCAAACATTTGTGATAGCACATAATTGTACAGATAAAACAGCGGAAAAAGCAAGAAATGCTGGTGCTATTGTTTATGAATATTCTAATTCTAGCGAAAGAACTAAGGGTTATGCATTAAAAAAAATATTTGAATTCATAGAACAAGACTATGGTATACAAAGTTTTGATGGATATCATATATTTGATGCGGACAATATAGTTGATAGTCTATATTTTGAAAAAATGAATGATGCTTTTTTACACTATGATAAAAAAAATGTAATTACTTCATTTAGAAATACTAAAAATTTTGGTAAAAGCATTCAAACAATTAATTATGGACTTATGTTTATGTTCCATACAACCATTGAATCAAACGCTAGAATGAAGTTTAAGATGTCTTCAAAAATTTTGGGTTCTGGTTTTTTAATTAGCAATGAAATGGTTAAAGATGGTTGA", "aa": "MKFKTQTQKLKYGIVICARNEEKVIAELVKSIKNSQYDQDKLQTFVIAHNCTDKTAEKARNAGAIVYEYSNSSERTKGYALKKIFEFIEQDYGIQSFDGYHIFDADNIVDSLYFEKMNDAFLHYDKKNVITSFRNTKNFGKSIQTINYGLMFMFHTTIESNARMKFKMSSKILGSGFLISNEMVKDG", "aa_hexdigest": "bd160d29b4c432524c9c95793c6169ac", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 717244, "stop": 718227, "observations": { "mutation": [ 717381, 717405, 717423, 717549, 718146 ], "stop": [ 717381, 717405, 717423, 717549, 718146 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFI2", "query_cov": 1.0, "subject_cov": 0.43488372093023253, "identity": 0.995, "score": 367.0, "evalue": 7.11e-125, "gene_start": 1, "gene_end": 187, "reference_start": 40, "reference_end": 226, "reference_sequence": "MVMVYQNVVYIVMPIIVAIFLIFSFQYWFVLFFGLFAKPVKFKTQTQKLKYGIVICARNEEKVIAELVKSIKNSQYDQDKLQTFVIAHNCTDKTAEKARNAGAIVYEYSNSSERTKGYALKKIFEFIEQDYGIQSFDGYHIFDADNIVDSLYFEKMNDAFLHYDKKNVITSFRNTKNFGKSIQTINYGLMFMFHTTIESNARMKFKMSSKILGSGFLISNEMVKDGWNITIPSDDSDFTVEQISEGKNVKYCDEAMFYDEQPTKFKDMWRQRLRWVFGNRIVWRKRFKSLIKNIFSSKKEDDKKISKSSSIDILFMISPISFIGFLILLLNILLVSLGPIFGANATQMWIDWAISFSISHAIIYLILFVIGIITYARGRKRVQRVPWWKMIISLFIWPLFLALYFPLQIVAIFKNINKFNWTQIEHNKNI" }, "paralog": false, "identity": 0.981651376146789, "score": 628.0, "evalue": 3.9e-230, "description": "Internal stop codon at 717381, 717405, 717423, 717549, 718146. Nonsense mutation around 717381, 717405, 717423, 717549, 718146.", "psc": { "uniref90_id": "UniRef90_C4XFI2", "product": "Glycosyltransferase", "uniref50_id": "UniRef50_C4XFI2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFI2", "UniRef:UniRef50_C4XFI2" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFI2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI2" ], "product": "Glycosyltransferase" } }, "id": "OAIKOHHJFB_1298", "locus": "GCA000209735_01284" }, { "type": "cds", "sequence": "contig_1", "start": 718774, "stop": 719034, "strand": "-", "frame": 2, "gene": null, "product": "Transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI3", "UniRef:UniRef90_C4XFI3" ], "nt": "ATGCGATTATGCGAACGAGATGCAGATTCAACTAAAGAAGCGTTAATACATATGATTGATGAACATGAATTAAATATTAATACTTTAACCATTGACAATGGTTCAGAAAATTACACTTTAGATCAGATCAACACTATTAAGCAAATATATCATTGCGATGCTTTCAATTCAAGTCAAAAAGGTAGAATTAAAAATTGTAATAAAATGATAAGAAAATATAGACCAAAATCAATTACTTTTGATAATTTAACACATGAATGA", "aa": "MRLCERDADSTKEALIHMIDEHELNINTLTIDNGSENYTLDQINTIKQIYHCDAFNSSQKGRIKNCNKMIRKYRPKSITFDNLTHE", "aa_hexdigest": "032228743de9e81b7b36b2298fecea41", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 718627, "stop": 719034, "observations": { "mutation": [ 718776 ], "stop": [ 718776 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFI3", "query_cov": 1.0, "subject_cov": 0.6370370370370371, "identity": 1.0, "score": 175.0, "evalue": 5.46e-55, "gene_start": 1, "gene_end": 86, "reference_start": 1, "reference_end": 86, "reference_sequence": "MRLCERDADSTKEALIHMIDEHELNINTLTIDNGSENYTLDQINTIKQIYHCDAFNSSQKGRIKNCNKMIRKYRPKSITFDNLTHEWIEIINEKINNFVRHEVDDFPFAMSANQFEKFIQENKHLYPSFYTNLYA" }, "paralog": false, "identity": 0.9925925925925926, "score": 278.0, "evalue": 6.12e-98, "description": "Internal stop codon at 718776. Nonsense mutation around 718776.", "psc": { "uniref90_id": "UniRef90_C4XFI3", "product": "Transposase", "uniref50_id": "UniRef50_C4XFI3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFI3", "UniRef:UniRef50_C4XFI3" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFI3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI3" ], "product": "Transposase" } }, "id": "OAIKOHHJFB_1299", "locus": "GCA000209735_01285" }, { "type": "cds", "sequence": "contig_1", "start": 718989, "stop": 719234, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCCAATAAGTCTGTAGGAAAGGGAGAAAGGCATAAAAAAGACCCTTCAATTCCAAAGAAAAGAAGAGTCGGATTACATATAAGTTTAAGACCAGAAATTACAACAATGTTCGATTTTGAATTTGATACAGTACATGGCAAAAGCGATGATTTATATCATATTGCTGTTTTAATGAATAGAGCAACACGAAATTTTATATGCGATTATGCGAACGAGATGCAGATTCAACTAAAGAAGCGTTAA", "aa": "MPNKSVGKGERHKKDPSIPKKRRVGLHISLRPEITTMFDFEFDTVHGKSDDLYHIAVLMNRATRNFICDYANEMQIQLKKR", "aa_hexdigest": "bb5b4b39f4644efdc6d01c57d7d58757", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9499.922899999992, "isoelectric_point": 9.774712562561035 }, "id": "OAIKOHHJFB_1300", "locus": "GCA000209735_01286" }, { "type": "cds", "sequence": "contig_1", "start": 719439, "stop": 719915, "strand": "-", "frame": 3, "gene": null, "product": "Transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI4", "UniRef:UniRef90_C4XFI4" ], "nt": "GTGAATCTCACTTTTTTTGTTTTGTCCTTGTATAATATAAATTGCATCAAAAGAATTAAAAATAAAAATAATGAATTTATAACTGAATATATACATGTTTTTGTCGAAAAATACGATCTTTTAACATTTATGAATTCGTTAAATAGAAATAAATTACATCCCAATAATGTAAGAATTGAAACAGTTATTAAAGCACTAGAAATGTTTAAAGAATTAAATAACTTTAAGAATATTCATAAATCTTTAAATTTAAAACGCGAAACATTTCAAAAACTATTTGATGACTTATCAGATGATAGAAAAATGATTTCCAAAACCGATAAATTTTTTAAAGGTTTGACGTGCCCACATTGTGGGGCTTATAAGCCCTTAGTTACAATTTTGAGTATAAAAAATAAAATTTCAGTTTTAAATAAATACAGAAATTGTAGAAGAATAATAGTTAATCAAAAAATTAAAAATAAACACAAAGATTGA", "aa": "MNLTFFVLSLYNINCIKRIKNKNNEFITEYIHVFVEKYDLLTFMNSLNRNKLHPNNVRIETVIKALEMFKELNNFKNIHKSLNLKRETFQKLFDDLSDDRKMISKTDKFFKGLTCPHCGAYKPLVTILSIKNKISVLNKYRNCRRIIVNQKIKNKHKD", "aa_hexdigest": "bae4b8e243c6510ed992a4b617416322", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 719136, "stop": 719957, "observations": { "mutation": [ 719441 ], "stop": [ 719441 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFI4", "query_cov": 1.0, "subject_cov": 0.4906832298136646, "identity": 0.9940000000000001, "score": 313.0, "evalue": 1.15e-105, "gene_start": 1, "gene_end": 158, "reference_start": 15, "reference_end": 172, "reference_sequence": "MKCVKVIKARPTILVNLTFFVLSLYNINCIKRIKNKNNEFITEYIHVFVEKYDLLTFMNSLNRNKLHPNNVRIETVIKALEMFKELNNFKNIHKSLNLKRETFQKLFDDLSDDRKMISKTDKFFKGLTCPHCGAYKPLVTILSIKNKISVLNKYRNCRRIIVNQKIKNKHKDWIKYINQNSNVYDRLCNENRKNSLRTFCQKSVRTLIDEAKEKCLKIKFPHFTTIYKNINILIFDINISILPNKSVGKGERHKKDPSIPKKRRVGLHISLRPEITTMFDFEFDTVHGKSDDLYHIAVLMNRATRNFICDYANEMQIQLKKR" }, "paralog": false, "identity": 0.9926739926739927, "score": 549.0, "evalue": 5.48e-201, "description": "Internal stop codon at 719441. Nonsense mutation around 719441.", "psc": { "uniref90_id": "UniRef90_C4XFI4", "product": "Transposase", "uniref50_id": "UniRef50_C4XFI4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFI4", "UniRef:UniRef50_C4XFI4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFI4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI4" ], "product": "Transposase" } }, "id": "OAIKOHHJFB_1301", "locus": "GCA000209735_01287" }, { "type": "cds", "sequence": "contig_1", "start": 719954, "stop": 720946, "strand": "-", "frame": 1, "gene": "galE", "product": "UDP-glucose 4-epimerase GalE", "db_xrefs": [ "EC:5.1.3.2", "GO:0003978", "GO:0006012", "RefSeq:WP_013526609.1", "SO:0001217", "UniParc:UPI0001F59A9D", "UniRef:UniRef100_C4XFI5", "UniRef:UniRef50_A0A507SK18", "UniRef:UniRef90_C4XFI5" ], "nt": "ATGACTTATTTATTAATTGGTGGAGCAGGTTATATCGGAAGCCATGTAGCTGAAGTTATAAATAAAGAAAATCAAAATAAAGTAATTATTTATGATAATCTTTCAACAGGATTTAAAGAATTTATTGAAGAAAAAAGTAAATTCATTCATGGAGATATTTTAGATATAAAAACATTAGATTTAACTTTTAAAAAAAATAAAATAGATGTAGTAATTTATTTAGCAGGTTTAATTAAAGTTGGTGAAAGTGTACAAAAACCACTTGACTATTATGAAACCAATATTCAAGGCTTAGTAAATGTTTTAAAAACAATGAAGAAGCATAATGTTAATAATTTGGTATTTTCTTCATCAGCAGCAGTATATGGTAATAACTCATTACACGATGGTTATTTTTTTGAAGAAGACCCAAAAGAACCATGTAGTCCTTATGGTAGAACCAAATATTTTGGAGAACAAATTATCAAAGACTTTGCTAATACAAATAGTGATTTCAAATATACTTTTTTAAGATACTTCAATGTTGCCGGTGCTTCAAAAAGTAAAAGAATTGGTTATTTAACCAAAAATGGTGTTGCACCGACTCATCTTATACCTGCTATTAGTTATTATGCTTTTGGTTTAATTCCCGAATTCAAAATTTTTGGTAATGACTATAACACACCTGATGGGACATGCATAAGAGATTATGTATATGTATATGAATTAGCAGAATTACATTTATTAACTGCAAAAAAAATGATTAAAGAAAAAACTTCATTATATTACAACATTGGTAGCTCAAAAGGCTTTTCAAATCTTGAAGTTGTAAATGCTTTTGAAAAAAATTTAAGCAAGAAATTAAATATTGAATATGGTCTAAGAAGAGCAGGAGACCCTGATAAATTAATAGCTTCAAATAAAAAAATATGTAAAGAATTAAATTATGAAGTAAAAACTAATTTAGATGAAATAATTTCTAGTGAGATTGAATTTAGAAAAGAACATTTATAA", "aa": "MTYLLIGGAGYIGSHVAEVINKENQNKVIIYDNLSTGFKEFIEEKSKFIHGDILDIKTLDLTFKKNKIDVVIYLAGLIKVGESVQKPLDYYETNIQGLVNVLKTMKKHNVNNLVFSSSAAVYGNNSLHDGYFFEEDPKEPCSPYGRTKYFGEQIIKDFANTNSDFKYTFLRYFNVAGASKSKRIGYLTKNGVAPTHLIPAISYYAFGLIPEFKIFGNDYNTPDGTCIRDYVYVYELAELHLLTAKKMIKEKTSLYYNIGSSKGFSNLEVVNAFEKNLSKKLNIEYGLRRAGDPDKLIASNKKICKELNYEVKTNLDEIISSEIEFRKEHL", "aa_hexdigest": "ee1e43407a438734a3d2e3aa37eace2f", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001F59A9D", "ncbi_nrp_id": "WP_013526609.1", "uniref100_id": "UniRef100_C4XFI5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59A9D", "RefSeq:WP_013526609.1", "UniRef:UniRef100_C4XFI5" ] }, "ips": { "uniref100_id": "UniRef100_C4XFI5", "uniref90_id": "UniRef90_C4XFI5" }, "psc": { "uniref90_id": "UniRef90_C4XFI5", "gene": "galE", "product": "UDP-glucose 4-epimerase GalE", "ec_ids": [ "5.1.3.2" ], "uniref50_id": "UniRef50_A0A507SK18", "go_ids": [ "GO:0003978", "GO:0006012" ] }, "pscc": { "uniref50_id": "UniRef50_A0A507SK18", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A507SK18" ], "product": "UDP-glucose 4-epimerase" }, "genes": [ "galE" ], "id": "OAIKOHHJFB_1302", "locus": "GCA000209735_01288" }, { "type": "cds", "sequence": "contig_1", "start": 720959, "stop": 721390, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAACATTTTTTCCAAATGTAAGAATTTTAAAAGAACTTTGATTTATTCTATAATTTATCATAGAAAAACCAATTCATTAACTTCATTTGATAAAAACTATATCAATAAAATTGAATGTATCAACCAAGCCTACAAATTTAATTTAGAAAACATAGTATCAAATTTAAAAAATGATGTAAGTTTTAAAATTCTTAAAAAAACTGTAAATTCCTTATTTATGGAACATTTAAGTTTGATTAGTGTTTACTATATCAATTTAAAAAATCGAAATTTAAAAAAAGAATTTAAAAAATTCATCAAAGATATTTATCCTTCAATTTTAGAAATATATAATGGTTTTGATGTAAAAATCAAATATCGAAGAATTTCTGATTTACCAGTTTTTGTTACTAAATTTTTATATAAAATACCAAAAGTTAAAAAATAG", "aa": "MGNIFSKCKNFKRTLIYSIIYHRKTNSLTSFDKNYINKIECINQAYKFNLENIVSNLKNDVSFKILKKTVNSLFMEHLSLISVYYINLKNRNLKKEFKKFIKDIYPSILEIYNGFDVKIKYRRISDLPVFVTKFLYKIPKVKK", "aa_hexdigest": "1d3a3c88af45e7ccdb2d5b35d0aa79f8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17170.183899999996, "isoelectric_point": 9.956835746765137 }, "id": "OAIKOHHJFB_1303", "locus": "GCA000209735_01289" }, { "type": "cds", "sequence": "contig_1", "start": 721472, "stop": 721966, "strand": "-", "frame": 1, "gene": null, "product": "Glycosyltransferase 2-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI6", "UniRef:UniRef90_C4XFI6" ], "nt": "ATGAATAGGCAAAAAACTTTATATCAACCTAAAATTTCAATTTTAATTCCCTTGCATAATAGTGCTAAATATTTTAATTCCACTATTAAAACTATTCTTAATCAAAGTTATGAAAATTTAGAAATTTTATTCCATGATGATGCCTCTAATGATGAAACAAGAAAAATCTTAGAAGATTTGACCATTAAAGATTCTAGAGTTAAATATATCTTTTCTGATACTAATATTGGTTCAGGATATTCAAGAAACAAATTAATTGAAGCTTCTACCGGAGATTTCATTTTTTTCATCGATGATGATGATGAATTTTTAAGTCCTAAAATAATAGAACGTTGTGTTAAATATTTAAAGCCTAATTTAGAAATACTTTCAACACAATTTAAATATAAATTTGATGATTTAGGTATTAAAATACCTATCAATAATATTGTTAAAACATTGAAAATCAATCCTTCACCAATGCATTATTATTTGAATACCACAACTTTTGCTTGA", "aa": "MNRQKTLYQPKISILIPLHNSAKYFNSTIKTILNQSYENLEILFHDDASNDETRKILEDLTIKDSRVKYIFSDTNIGSGYSRNKLIEASTGDFIFFIDDDDEFLSPKIIERCVKYLKPNLEILSTQFKYKFDDLGIKIPINNIVKTLKINPSPMHYYLNTTTFA", "aa_hexdigest": "819d4df325371f604d7a18fecc4fcf32", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "both", "pseudogene": { "start": 721169, "stop": 722038, "observations": { "mutation": [ 721474, 722014 ], "stop": [ 721474, 722014 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFI6", "query_cov": 1.0, "subject_cov": 0.4568245125348189, "identity": 1.0, "score": 327.0, "evalue": 1.19e-110, "gene_start": 1, "gene_end": 164, "reference_start": 25, "reference_end": 188, "reference_sequence": "MFIFIDNYWMFSKISNIYYIKRKNMNRQKTLYQPKISILIPLHNSAKYFNSTIKTILNQSYENLEILFHDDASNDETRKILEDLTIKDSRVKYIFSDTNIGSGYSRNKLIEASTGDFIFFIDDDDEFLSPKIIERCVKYLKPNLEILSTQFKYKFDDLGIKIPINNIVKTLKINPSPMHYYLNTTTFAWGHFFNKKFLYDIDVNFSKVKKYEDIAEMGNIFSKCKNFKRTLIYSIIYHRKTNSLTSFDKNYINKIECINQAYKFNLENIVSNLKNDVSFKILKKTVNSLFMEHLSLISVYYINLKNRNLKKEFKKFIKDIYPSILEIYNGFDVKIKYRRISDLPVFVTKFLYKIPKVKK" }, "paralog": false, "identity": 0.9896193771626297, "score": 574.0, "evalue": 2.4e-210, "description": "Internal stop codon at 721474, 722014. Nonsense mutation around 721474, 722014.", "psc": { "uniref90_id": "UniRef90_C4XFI6", "product": "Glycosyltransferase 2-like domain-containing protein", "uniref50_id": "UniRef50_C4XFI6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFI6", "UniRef:UniRef50_C4XFI6" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFI6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI6" ], "product": "Glycosyltransferase 2-like domain-containing protein" } }, "id": "OAIKOHHJFB_1304", "locus": "GCA000209735_01290" }, { "type": "cds", "sequence": "contig_1", "start": 722146, "stop": 722508, "strand": "-", "frame": 2, "gene": null, "product": "AB hydrolase-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI7", "UniRef:UniRef90_C4XFI7" ], "nt": "TTGATTCCTGCTAATGTTGAAGATGCTAGAAATTCATTAAAAGCATTGGTTTATAATCCTGAAGATTATTATTTAAGAAACTTGGATTATAATGCTGAAAAATTAGCGAGCAAATATAATGAAAACAAAGAAACTTTTTCTTATCTATTAAATGAACAAATTTTAAATAAAGATTATTTATTAAAACATGTAAAGCCTTTATTTGCAAATCCAAATGGTTGTGTAATTATTTATGGTGAAAATGATGAATTTGTGACACCAAAAGAAATTAAAAAAATAGAAGAAGATTATGGTTATAAAATTGTGCAATTAAATGAATGTGGACATGCTGCATTTTATCAAAAATTAAATACCATTTATTGA", "aa": "MIPANVEDARNSLKALVYNPEDYYLRNLDYNAEKLASKYNENKETFSYLLNEQILNKDYLLKHVKPLFANPNGCVIIYGENDEFVTPKEIKKIEEDYGYKIVQLNECGHAAFYQKLNTIY", "aa_hexdigest": "30b36ee128c2e7586cf6ca2c83cdd545", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 722116, "stop": 722808, "observations": { "mutation": [ 722148, 722511 ], "stop": [ 722148, 722511 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFI7", "query_cov": 1.0, "subject_cov": 0.40955631399317405, "identity": 0.992, "score": 248.0, "evalue": 4.41e-81, "gene_start": 1, "gene_end": 120, "reference_start": 164, "reference_end": 283, "reference_sequence": "MYKRQSIELLGETIYYERIDCFQKEKRPATVLLVHGFNSDSNTFTRFIDFAEVVAENTPDNDELREEYRNFHRFNLYALDLPGCGKSGKNSQITLDLYNKIVVEFINTVLKDHEVILISHSMGSVPCLYAKKECKNVTRLFMVAPLNYALVDDKQKLINLKKWLIPANVEDARNSLKALVYNPEDYYLRNLDYNAEKLASKYNENKETFSYLLNEQILNKDYLLKHVKPLFANPNGCVIIYGENDEFVTPKEIKKIEEDYGYKIVQLNECGHAAFYQKLNTIYWIVQKELNYK" }, "paralog": false, "identity": 0.991304347826087, "score": 462.0, "evalue": 5.48e-168, "description": "Internal stop codon at 722148, 722511. Nonsense mutation around 722148, 722511.", "psc": { "uniref90_id": "UniRef90_C4XFI7", "product": "AB hydrolase-1 domain-containing protein", "uniref50_id": "UniRef50_C4XFI7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFI7", "UniRef:UniRef50_C4XFI7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFI7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI7" ], "product": "AB hydrolase-1 domain-containing protein" } }, "id": "OAIKOHHJFB_1305", "locus": "GCA000209735_01291" }, { "type": "cds", "sequence": "contig_1", "start": 722509, "stop": 722997, "strand": "-", "frame": 2, "gene": null, "product": "AB hydrolase-1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI7", "UniRef:UniRef90_C4XFI7" ], "nt": "ATGTATAAAAGACAATCAATTGAACTTTTAGGCGAAACAATTTATTATGAAAGAATAGATTGTTTTCAAAAAGAAAAAAGACCAGCAACTGTTTTACTTGTGCACGGTTTTAATTCTGATTCAAATACCTTTACTAGATTTATTGATTTTGCTGAAGTAGTGGCCGAAAATACACCCGATAATGACGAATTAAGAGAAGAATATAGAAACTTTCATCGTTTCAATTTATATGCTTTAGATTTGCCTGGTTGTGGAAAATCTGGAAAAAATAGTCAAATAACACTTGATTTATATAACAAAATAGTAGTTGAATTTATTAACACAGTTTTAAAAGACCACGAAGTAATTTTAATTTCGCATTCAATGGGTTCAGTACCTTGTTTGTATGCTAAAAAAGAATGCAAAAACGTAACTAGGTTATTTATGGTAGCTCCTTTAAATTATGCTTTAGTTGACGATAAGCAAAAACTAATTAATCTTAAAAAATGA", "aa": "MYKRQSIELLGETIYYERIDCFQKEKRPATVLLVHGFNSDSNTFTRFIDFAEVVAENTPDNDELREEYRNFHRFNLYALDLPGCGKSGKNSQITLDLYNKIVVEFINTVLKDHEVILISHSMGSVPCLYAKKECKNVTRLFMVAPLNYALVDDKQKLINLKK", "aa_hexdigest": "d37823e7e29b412ec68943aaa9590935", "start_type": "ATG", "rbs_motif": "AGGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 722206, "stop": 722997, "observations": { "mutation": [ 722511 ], "stop": [ 722511 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFI7", "query_cov": 1.0, "subject_cov": 0.552901023890785, "identity": 1.0, "score": 331.0, "evalue": 5.17e-113, "gene_start": 1, "gene_end": 162, "reference_start": 1, "reference_end": 162, "reference_sequence": "MYKRQSIELLGETIYYERIDCFQKEKRPATVLLVHGFNSDSNTFTRFIDFAEVVAENTPDNDELREEYRNFHRFNLYALDLPGCGKSGKNSQITLDLYNKIVVEFINTVLKDHEVILISHSMGSVPCLYAKKECKNVTRLFMVAPLNYALVDDKQKLINLKKWLIPANVEDARNSLKALVYNPEDYYLRNLDYNAEKLASKYNENKETFSYLLNEQILNKDYLLKHVKPLFANPNGCVIIYGENDEFVTPKEIKKIEEDYGYKIVQLNECGHAAFYQKLNTIYWIVQKELNYK" }, "paralog": false, "identity": 0.9961977186311787, "score": 532.0, "evalue": 7.79e-195, "description": "Internal stop codon at 722511. Nonsense mutation around 722511.", "psc": { "uniref90_id": "UniRef90_C4XFI7", "product": "AB hydrolase-1 domain-containing protein", "uniref50_id": "UniRef50_C4XFI7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFI7", "UniRef:UniRef50_C4XFI7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFI7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFI7" ], "product": "AB hydrolase-1 domain-containing protein" } }, "id": "OAIKOHHJFB_1306", "locus": "GCA000209735_01292" }, { "type": "cds", "sequence": "contig_1", "start": 723286, "stop": 723990, "strand": "-", "frame": 2, "gene": null, "product": "BPL/LPL catalytic domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FB30" ], "nt": "ATGAAAATTTTTAGAATTAAGGAAACTTCACCTTATGTTACTTTAGCTTTAGAAAATATTATTATGCATGATCATGATTTAAAAGGTGATGTACTTTTAATTTATCAACATGATAATGCTATTATTATTGGTAACAATCAAAATGCATATGAAGAAATTAACCGTTCTTTTGTAAAAGAAAATAATATTAAATTAGCTCGTAGAATGTCTGGCGGTGGAGCTGTGTATCATGATTTAGGCAATATTAATTTTAGTTTCATAACTGACTATGATAAAAAAGGCGGTTATGAAAGATTTCTAACTCCAATTATTGCTTTTCTTAAAAGCTTAGGTCTAGATGCTCAATTTAAAGGTCGCAATGATGTTGTGGTTAATGGCTGCAAAATAAGTGGTAACGCCCAATATATTAGTAATAATCGAATAGTAAGCCATGGAACATTATTATTTGATGTTGATTTAACAAAATTATCGAAAGCACTTAATCCTGCAAGAATTAAGTATGAATCTAAAGGCATTCAATCAGTTAGATCTAGAGTAGTTAATATTTATGATGAATTATTAAACAAAATGACAGCTGAAGAATTTATTAGTCGTCTAATTAATTATTTTGTTAAGAATTTTAAAGGAGAATTTATTGATATTGATTATGCAAAATATCAAGAACAATTAAACTCTTTACAACAAAAATTTGCTTCTGAAGAATGA", "aa": "MKIFRIKETSPYVTLALENIIMHDHDLKGDVLLIYQHDNAIIIGNNQNAYEEINRSFVKENNIKLARRMSGGGAVYHDLGNINFSFITDYDKKGGYERFLTPIIAFLKSLGLDAQFKGRNDVVVNGCKISGNAQYISNNRIVSHGTLLFDVDLTKLSKALNPARIKYESKGIQSVRSRVVNIYDELLNKMTAEEFISRLINYFVKNFKGEFIDIDYAKYQEQLNSLQQKFASEE", "aa_hexdigest": "15eb3472949c422187a5f6ed7da5e547", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00111975EB", "query_cov": 0.9658119658119658, "subject_cov": 0.9576271186440678, "identity": 0.6729999999999999, "score": 311.0, "evalue": 7.48e-105, "valid": false, "product": "lipoate--protein ligase", "uniref50_id": "UniRef50_A0A432FB30" }, "pscc": { "uniref50_id": "UniRef50_A0A432FB30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FB30" ], "product": "BPL/LPL catalytic domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_1307", "locus": "GCA000209735_01293" }, { "type": "cds", "sequence": "contig_1", "start": 724674, "stop": 725201, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTACAGCACAAACTACAGGTATAGAAAACAAAAAATCTAAACCAAAAGACAATAGCCGTAAGGGAAATTTTCGTAAGATTCTAAGTAAAATCTCTGGTGCTTTCATGTTACCAATTTCCGTTATGTCTATAGCTGGTCTATTTCTAGGGGTTGGTGCAACAATATCAAGTCAATCAGCGATTGTTCTTAACAACAATCCATTATCATCTGCTGCTAAAGCTGGTATTGTAATTGGTGACTTTATTAAGTTATTAGGTGACCCAATATTTGGAGCAATGCCGCTATTGTTTGCCGCTGCCTTTGTTGTGGCATTTACCGACGAAGCCGGAGTCGGTGTCTTCGCCGCAATAGTTGGATACTTAGTATTCTCAGCAATACAAGCTCCATTTATTCAAAGTGTTATAGAAAAAACTAATATTGATGGCACTAAACAAGCTAGTGAATTAATAGGTAAAACAATACTTAAAGATGATAAAACAGGACAACTTTACTATGAAACATTAAAAGGATATAACATTCTTTGA", "aa": "MTTAQTTGIENKKSKPKDNSRKGNFRKILSKISGAFMLPISVMSIAGLFLGVGATISSQSAIVLNNNPLSSAAKAGIVIGDFIKLLGDPIFGAMPLLFAAAFVVAFTDEAGVGVFAAIVGYLVFSAIQAPFIQSVIEKTNIDGTKQASELIGKTILKDDKTGQLYYETLKGYNIL", "aa_hexdigest": "a215c937b1056fca8d3d7aade24d8e93", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18467.3631, "isoelectric_point": 9.42574520111084 }, "id": "OAIKOHHJFB_1308", "locus": "GCA000209735_01294" }, { "type": "cds", "sequence": "contig_1", "start": 725202, "stop": 725441, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAGGTAAATTTGGTGCTGAAGTTAAACAAGTCGTTGGTTCTTCATTAGGATTCAAAACTCTTCAAACTTCAGTTTTCGGTGGTATTTTTGTTGGTTTAATAGTTTCATACTTATACAACAAATTACACACAATTAAACTTCCAACTGTTATTTCATTCTTTGGAGGAAAACGTTTTGTTTCATTAATAACAATAGTTGCTATGATTCCATTAGCATTCTTATTCCTCCTTATATGA", "aa": "MTGKFGAEVKQVVGSSLGFKTLQTSVFGGIFVGLIVSYLYNKLHTIKLPTVISFFGGKRFVSLITIVAMIPLAFLFLLI", "aa_hexdigest": "6fb69cb74b120360696917bdbe8af925", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8585.338099999994, "isoelectric_point": 10.218255043029785 }, "id": "OAIKOHHJFB_1309", "locus": "GCA000209735_01295" }, { "type": "cds", "sequence": "contig_1", "start": 725567, "stop": 725770, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGAAATTTAATTTCATTACACCAATAGCAGCATATGAGTCACCCATTCATTCATTGTGTTTAGAAGCCATCAAAGCTTTTCAACCATTGTACGCAGTTAATGCAGTAGCATCTCCTGAAAAAGTATTTCCTCTTGCTTCTCAATCTTCAAGCGCTTTAAGAATATCACCGCCTGCATTTGTGTATCAAAGAGGTGCATAA", "aa": "MLKFNFITPIAAYESPIHSLCLEAIKAFQPLYAVNAVASPEKVFPLASQSSSALRISPPAFVYQRGA", "aa_hexdigest": "0effe25a92e7635d023a379d4464e728", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7269.399700000002, "isoelectric_point": 8.978850364685059 }, "id": "OAIKOHHJFB_1310", "locus": "GCA000209735_01296" }, { "type": "cds", "sequence": "contig_1", "start": 725868, "stop": 726050, "strand": "+", "frame": 3, "gene": null, "product": "PTS transporter subunit EIIC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF38607", "UniRef:UniRef90_UPI002FF38607" ], "nt": "ATGCAAGGCAAATACTCATTCATGATTCTAGGATTACCGGGTGCAGCTGTTGCTATGATTTTAGCGGCTCCTAAAGAAAATAGAAAAGTGGCAGCTGGTACAGTTATTCCTGCAGCTCTTACTTCAGTTATTACTGGTGTTACAGAACCTATTGAATTTACATTCTTATTCTTAGCACCATGA", "aa": "MQGKYSFMILGLPGAAVAMILAAPKENRKVAAGTVIPAALTSVITGVTEPIEFTFLFLAP", "aa_hexdigest": "a333f9de734758970e8316a67670feb8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 725850, "stop": 726098, "observations": { "mutation": [ 726048 ], "stop": [ 726048 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FF38607", "query_cov": 0.9833333333333333, "subject_cov": 0.7108433734939759, "identity": 0.831, "score": 98.2, "evalue": 1.18e-25, "gene_start": 2, "gene_end": 60, "reference_start": 8, "reference_end": 66, "reference_sequence": "MKIGRFADGKFSFMIFGLPAAAAAMILAAPKENRKLALGTVVPAAFTSIITGVTEPIEFTFLFLAPLLFWGFHALICIFIYVC" }, "paralog": false, "identity": 0.7439024390243902, "score": 130.0, "evalue": 4.81e-41, "description": "Internal stop codon at 726048. Nonsense mutation around 726048.", "psc": { "uniref90_id": "UniRef90_UPI002FF38607", "product": "PTS transporter subunit EIIC", "uniref50_id": "UniRef50_UPI002FF38607", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FF38607", "UniRef:UniRef50_UPI002FF38607" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FF38607", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF38607" ], "product": "PTS transporter subunit EIIC" } }, "id": "OAIKOHHJFB_1311", "locus": "GCA000209735_01297" }, { "type": "cds", "sequence": "contig_1", "start": 726276, "stop": 726542, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00367.25" ], "nt": "TTGGCAACCCCAGGTCGTGGTGAAACAGTTAGATTATTCACCAAAAAAGACTTCCAAGCTAAACAAGATGGAAATGATGAAAATTCTTCAAGTAAAGATACTTCAGCAAGAAACGCAAGAGAATATGCAATGATTCTTGCTTTAGGCGGTCCAAAAAACATCGTTAACACAAATAACTGTGCTTCAAGATTAAGATATGATATTAACGATAGAAACTTAGTAAATGAAGTTGAATGTAAGGCAGCAGGTGCAGTTGCACTTAAATGA", "aa": "MATPGRGETVRLFTKKDFQAKQDGNDENSSSKDTSARNAREYAMILALGGPKNIVNTNNCASRLRYDINDRNLVNEVECKAAGAVALK", "aa_hexdigest": "85e4901081aca55cc3e0ad07257329e8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00367.25", "name": "PTS_EIIB", "length": 31, "aa_cov": 0.3522727272727273, "hmm_cov": 1.0, "evalue": 1.8379386183769403e-12, "score": 42.57704544067383, "start": 45, "stop": 75 } ], "seq_stats": { "molecular_weight": 9604.646399999994, "isoelectric_point": 9.061563301086426 }, "id": "OAIKOHHJFB_1312", "locus": "GCA000209735_01298" }, { "type": "cds", "sequence": "contig_1", "start": 726591, "stop": 726782, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCTAACATTAGAAAAATTTTAGCTTCAGGTGAATTAGATGACATTGTTGTTGAAGCAAAAAGCGAACCAACACAACCAGTTGTAGTAGTTGAAGCTCCAACACAAGAACAACCTACTGTTCAAGAAACCCCAGCAGTTACACCTGAACCTCAAGTTGAAACATCATCAATGACAGAAACTAAATGA", "aa": "MNANIRKILASGELDDIVVEAKSEPTQPVVVVEAPTQEQPTVQETPAVTPEPQVETSSMTETK", "aa_hexdigest": "88e98fbed809d246d02c73f7a196e5b2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6789.516500000002, "isoelectric_point": 4.16302433013916 }, "id": "OAIKOHHJFB_1313", "locus": "GCA000209735_01299" }, { "type": "cds", "sequence": "contig_1", "start": 727116, "stop": 727274, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTATGTACAGTAGATGTTGAATACTTATCAAACCAAAATAAAGTAAGTGATGTTATCGTTGTAATTCTTAATGAATCAGAACACAAACACATTATTGATACAGCTCTTGGCGAAAAAATGACTAAACGTGCTGACAAATTAATGCTTGTTAAATAA", "aa": "MLCTVDVEYLSNQNKVSDVIVVILNESEHKHIIDTALGEKMTKRADKLMLVK", "aa_hexdigest": "0245acafaf42e63c52f2da9e2ed48dbf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5913.903799999999, "isoelectric_point": 6.024785423278809 }, "id": "OAIKOHHJFB_1314", "locus": "GCA000209735_01300" }, { "type": "cds", "sequence": "contig_1", "start": 727688, "stop": 728092, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13306.12", "PFAM:PF13855.12" ], "nt": "TTGATTAAATTAGGTGATGGTGTTTTTTATGGTTGTAGAAGTCTAACAAAAATTATCTTCAACAGTCAACTTCAAATAATTGGAAAAGAAACTTTTAGCCATTGTAATTCAATAGAAGAAATAAACATTCCAGATACTGTAAATTTAAACACTGAAGGTGCTTTTGAAATGTGTTTTAATTTAAAAACCGTTCATATCCCAAATAACACAACAATAATTAAAACTAATGCCTTTAAACAGTGCGAATCATTAGAAACATTAGACTTAACAAATACCAACGTGAATCAAATACAAGCAAATACATTTAAAGGATGTACAAACCTAAAAGAAGTTAAGGTCAAAAAAGACACTCAAATAGACCCTCAAGCAAGCGATAATAATTCAATTTTTGTTTTTAATATTTAA", "aa": "MIKLGDGVFYGCRSLTKIIFNSQLQIIGKETFSHCNSIEEINIPDTVNLNTEGAFEMCFNLKTVHIPNNTTIIKTNAFKQCESLETLDLTNTNVNQIQANTFKGCTNLKEVKVKKDTQIDPQASDNNSIFVFNI", "aa_hexdigest": "c764be6894559c8a073df3ec6f6d9980", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13306.12", "name": "LRR_5", "length": 125, "aa_cov": 0.9253731343283582, "hmm_cov": 0.968, "evalue": 3.2656244820600276e-29, "score": 97.4892349243164, "start": 3, "stop": 126 }, { "id": "PF13855.12", "name": "LRR_8", "length": 51, "aa_cov": 0.373134328358209, "hmm_cov": 1.0, "evalue": 3.0135951862393853e-13, "score": 45.46135711669922, "start": 61, "stop": 110 } ], "seq_stats": { "molecular_weight": 15036.963899999975, "isoelectric_point": 5.628674507141112 }, "id": "OAIKOHHJFB_1315", "locus": "GCA000209735_01301" }, { "type": "cds", "sequence": "contig_1", "start": 728492, "stop": 729049, "strand": "-", "frame": 1, "gene": null, "product": "IS3 family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZVD0", "UniRef:UniRef90_UPI00003A869D" ], "nt": "TTGAAAAAGTCAACTTTTTATGAGATATTAAAATCACAAAATAAACCTGATAAAGATGAAAATTTAAAAAAGGTTATTTTTGACTTATTTAACTATAATAAAGGACTATACGGTTATAGACGTATTACTTTTGCTTTAAGAAATAAAGGAATAATAATCAATCATAAAAAAGTTCAAAAATTAATGAAAGCAATGAATATTTTCGGCAAAACGCTAAGAAGAAAAAATAAATATTCTTCATTCAAAGGTGATGCTCACAAAAACATTCCAAACTTGCTTTTAGATAAAGAAAATATCACAGAAGATTTCTTCAGATACAAAAGAAATTTTTCAAATAATAAATATTTGAAAATACTAGGAACAGATGTTACTGAATTTAAATTAAAAAATGATGAAAAAGCATATTTTTCTCCTGTAGTTGATTTTGAAAACAGAGAGATTTTAGGTTATTCGATTTCTAAATCGCCTAATTTAAGAATGGTTGGTAAAATGTTAGAAAACGTAGAAGAGAATGGCCACAGCTTAAAAAATGTATTATTACATTCTGATCAAGGATGA", "aa": "MKKSTFYEILKSQNKPDKDENLKKVIFDLFNYNKGLYGYRRITFALRNKGIIINHKKVQKLMKAMNIFGKTLRRKNKYSSFKGDAHKNIPNLLLDKENITEDFFRYKRNFSNNKYLKILGTDVTEFKLKNDEKAYFSPVVDFENREILGYSISKSPNLRMVGKMLENVEENGHSLKNVLLHSDQG", "aa_hexdigest": "e9ee43c1414342ade693b8ebf6d8de59", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00003A869D", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.935, "score": 347.0, "evalue": 1.23e-120, "valid": true, "product": "IS3 family transposase", "uniref50_id": "UniRef50_A0A448ZVD0" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZVD0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZVD0" ], "product": "Transposase, IS861" }, "genes": [], "id": "OAIKOHHJFB_1316", "locus": "GCA000209735_01302" }, { "type": "cds", "sequence": "contig_1", "start": 729115, "stop": 729405, "strand": "-", "frame": 2, "gene": null, "product": "Transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET5" ], "nt": "ATGGAAAACTCTTATTCAGCATGCTATTCAGTAATAGAAAAATACAAAAAGTTTGGTTATAATTCTTTTGCTATGGAAAAGAAAAAAGGAAGAAAATCTAAAATAAATTTAGATGCTCAAAAGGCAACAAATTTTAAAATCAATATTGAAAATAAAATAGAAAATAAAGATTTATTAATTAAACAATTAAAGGAAGAAAATAAAATACTCAAATTGGAGAATGCGATAGCAAAAAAAGTGAGCGCCTTGGTTCAATTGAAAGACTCACTAACAAAGAAAAATTCCAAATAA", "aa": "MENSYSACYSVIEKYKKFGYNSFAMEKKKGRKSKINLDAQKATNFKINIENKIENKDLLIKQLKEENKILKLENAIAKKVSALVQLKDSLTKKNSK", "aa_hexdigest": "ba35629af28b55b0957136a2608bd32f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XET5", "query_cov": 1.0208333333333333, "subject_cov": 0.9607843137254902, "identity": 0.765, "score": 129.0, "evalue": 4.04e-37, "valid": false, "product": "Transposase", "uniref50_id": "UniRef50_C4XET5" }, "pscc": { "uniref50_id": "UniRef50_C4XET5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET5" ], "product": "Transposase" }, "genes": [], "id": "OAIKOHHJFB_1317", "locus": "GCA000209735_01303" }, { "type": "cds", "sequence": "contig_1", "start": 729666, "stop": 729980, "strand": "-", "frame": 3, "gene": null, "product": "MPN499 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFJ3", "UniRef:UniRef50_UPI001F306D39", "UniRef:UniRef90_C4XFJ3" ], "nt": "ATGAGACATTTAGAAATTAAATATAATCCAGGGTATTTTAATTTCAATGGTGATCTAAGATTTAAAGCATTCAATGAGTTACAACGAGTACATAAAGTAGGTATCTCAATTAATCCTTCAGCGGTTAAAAATAAAGACAATTATTTCAAGTTTCATATTAGTGAAAATGTTGAAGTAGTGTTAGATGATTTATCACTAAAATTAATTAGTAAAGGTAGATCGTTTGCCTGTCCAATGCATTTCTTTGATGATTTATACTTAGAATATTTTGATGAGAAAAAAGTAACTAAAAATCAAAAATTGAGATTAACTTGA", "aa": "MRHLEIKYNPGYFNFNGDLRFKAFNELQRVHKVGISINPSAVKNKDNYFKFHISENVEVVLDDLSLKLISKGRSFACPMHFFDDLYLEYFDEKKVTKNQKLRLT", "aa_hexdigest": "439e9f6fe01074690a5db326f9afcb09", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F306D39", "query_cov": 0.8269230769230769, "subject_cov": 0.8269230769230769, "identity": 0.7559999999999999, "score": 139.0, "evalue": 6.47e-41, "valid": false, "uniref50_id": "UniRef50_UPI001F306D39" }, "pscc": { "uniref50_id": "UniRef50_UPI001F306D39", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F306D39" ] }, "genes": [], "truncated": "both", "pseudogene": { "start": 729615, "stop": 730103, "observations": { "mutation": [ 729668, 730016, 730055 ], "stop": [ 729668, 730016, 730055 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFJ3", "query_cov": 1.0, "subject_cov": 0.6419753086419753, "identity": 0.99, "score": 215.0, "evalue": 2.76e-70, "gene_start": 1, "gene_end": 104, "reference_start": 42, "reference_end": 145, "reference_sequence": "MTKIKVYKVNIIENRYWYCPSLFTFSRRLWASRPFATLEELMRHLEIKYNPGYFNFNGDLRFKAFNELQRVHKVGISINPSAVKDKDNYFKFHISENVEVVLDDLSLKLISKGRSFACPMHFFDDLYLEYFDEKKVTKNQKLRLTWRKYFFNIEVVEKEQTE" }, "paralog": false, "identity": 0.9753086419753086, "score": 318.0, "evalue": 3.24e-113, "description": "Internal stop codon at 729668, 730016, 730055. Nonsense mutation around 729668, 730016, 730055.", "psc": { "uniref90_id": "UniRef90_C4XFJ3", "product": "MPN499 family protein", "uniref50_id": "UniRef50_C4XFJ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFJ3", "UniRef:UniRef50_C4XFJ3" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFJ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFJ3" ] } }, "id": "OAIKOHHJFB_1318", "locus": "GCA000209735_01304" }, { "type": "cds", "sequence": "contig_1", "start": 730096, "stop": 730344, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGCCAATAGGAATATCAAAAGGTGAAACATTAAAAAAAATAGCTAAGATTAAAAATATTGATTTATCAAAAGCAATGGCTTTTGGTGATGCTGATAATGACATAAGTATGTTAAAATCTGTTAAAATTTCAGTTGCAATGGGTAATGCAACAGAGAATTTAAAGAAAGTTGCAACTTATGTAACAGATACAAATAATAATGATGGAATTGCCAAAATTATTAAAGAATTAGAGTTAGTAAATGACTAA", "aa": "MPIGISKGETLKKIAKIKNIDLSKAMAFGDADNDISMLKSVKISVAMGNATENLKKVATYVTDTNNNDGIAKIIKELELVND", "aa_hexdigest": "3df4e08f2e3fe2a5236892bf55f0f750", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 74, "aa_cov": 0.9024390243902439, "hmm_cov": 1.0, "evalue": 3.3071921768660846e-30, "score": 101.71113586425781, "start": 1, "stop": 74 } ], "seq_stats": { "molecular_weight": 8824.179999999997, "isoelectric_point": 7.9259527206420906 }, "id": "OAIKOHHJFB_1319", "locus": "GCA000209735_01305" }, { "type": "cds", "sequence": "contig_1", "start": 730567, "stop": 730953, "strand": "-", "frame": 2, "gene": null, "product": "Sucrose-6F-phosphate phosphohydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEN1", "UniRef:UniRef90_C4XFJ4" ], "nt": "ATGCTTATTATGGTCAAAATAAATAATATAGACTACTTCATTTTTGATTTAGATGGTACACTCTATGATGATAATAAAAACATCTTAGAATCAACCAAAAACACTTTAAAACAGCTTCAAAAGGAAAATAAAAATATAATAATTGCTACTGGTAGACCTTATTATATGAACTATAAATTAATTAAGGATTTAGATATTAAACTACCAATAATAGCAGTTAATGGGGCTATGATTTATGATCCTATCACTAAAGAAGTGCCTTATTTTCAAGGACTAGACAAAAAAGTTGTTCAAGAATTAATTAACTTTTTAGAAGAGAATAGAATTGATTTTTTAGCTTACGATATTAATGGTATGAAAGGTAATAATTTCTCGAACCCTTATTGA", "aa": "MLIMVKINNIDYFIFDLDGTLYDDNKNILESTKNTLKQLQKENKNIIIATGRPYYMNYKLIKDLDIKLPIIAVNGAMIYDPITKEVPYFQGLDKKVVQELINFLEENRIDFLAYDINGMKGNNFSNPY", "aa_hexdigest": "7c82ae5ddbbb9a93d9a8c8ed992b41db", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 730264, "stop": 730953, "observations": { "mutation": [ 730518, 730569 ], "stop": [ 730518, 730569 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFJ4", "query_cov": 1.0, "subject_cov": 0.44912280701754387, "identity": 1.0, "score": 257.0, "evalue": 1.08e-84, "gene_start": 1, "gene_end": 128, "reference_start": 1, "reference_end": 128, "reference_sequence": "MLIMVKINNIDYFIFDLDGTLYDDNKNILESTKNTLKQLQKENKNIIIATGRPYYMNYKLIKDLDIKLPIIAVNGAMIYDPITKEVPYFQGLDKKVVQELINFLEENRIDFLAYDINGMKGNNFSNPYWFEFMIYKNVVETNPYRWDYQEGSLKETCNQFNFAKILIITQNVPQEKIDSLLTIASKFPKQIYTVSSQKGVIDIMPIGISKGETLKKIAKIKNIDLSKAMAFGDADNDISMLKSVKISVAMGNATENLKKVATYVTDTNNNDGIAKIIKELELVND" }, "paralog": false, "identity": 0.9912663755458515, "score": 449.0, "evalue": 1.19e-162, "description": "Internal stop codon at 730518, 730569. Nonsense mutation around 730518, 730569.", "psc": { "uniref90_id": "UniRef90_C4XFJ4", "product": "Sucrose-6F-phosphate phosphohydrolase", "uniref50_id": "UniRef50_A0A3P8MEN1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFJ4", "UniRef:UniRef50_A0A3P8MEN1" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MEN1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEN1" ], "product": "Cof-like hydrolase" } }, "id": "OAIKOHHJFB_1320", "locus": "GCA000209735_01306" }, { "type": "cds", "sequence": "contig_1", "start": 730928, "stop": 731047, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATTAATACCTTAATAGTTTCAAATAATAGTGCTCCAACTGCGCAAGAAGCATTGATTGAAAAGCACTATAACCGTAAGCACGGAAAGAATGCTTATTATGGTCAAAATAAATAA", "aa": "MAINTLIVSNNSAPTAQEALIEKHYNRKHGKNAYYGQNK", "aa_hexdigest": "0f6872b9d7517e1b12f6900b7304aad9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4388.875100000002, "isoelectric_point": 9.60174388885498 }, "id": "OAIKOHHJFB_1321", "locus": "GCA000209735_01307" }, { "type": "cds", "sequence": "contig_1", "start": 731156, "stop": 731518, "strand": "-", "frame": 1, "gene": null, "product": "L-ribulose-5-phosphate 4-epimerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_R6LVT1" ], "nt": "ATGGTTATTAAACCAAGTGGTGTTTCTTATGACACACTTAAAGCTGATGACATGGTCATAACTGATTTAGATAACAATGTATTTGATTCTAAATATCGCCCTTCTTCAGACACACCTACTCATACCTTACTTTATAAAGAAAATCCTGAAATAAAAGGTATTGTTCATACTCACAGTCCTCATGCAGTAGCTTTTGCACAAGCAGGTAAAGATATTCCTTGCTTTGGTACAACACATGCAGATAATTTTTATGGACCAGTGCCATGTGCTAGAGCTCTAACTCCAAAAGAAATTGATGGAGAATATGAACACAATACAGGACTTGTAATAATAGAAACATTTAAAAAACGCAAGATTGACTGA", "aa": "MVIKPSGVSYDTLKADDMVITDLDNNVFDSKYRPSSDTPTHTLLYKENPEIKGIVHTHSPHAVAFAQAGKDIPCFGTTHADNFYGPVPCARALTPKEIDGEYEHNTGLVIIETFKKRKID", "aa_hexdigest": "bb6b4f2a7f0b646380327b2d7ceac778", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_R6LVT1", "query_cov": 0.8416666666666667, "subject_cov": 0.9901960784313726, "identity": 0.594, "score": 128.0, "evalue": 2.97e-36, "valid": false, "product": "L-ribulose-5-phosphate 4-epimerase", "uniref50_id": "UniRef50_R6LVT1" }, "pscc": { "uniref50_id": "UniRef50_R6LVT1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_R6LVT1" ], "product": "L-ribulose-5-phosphate 4-epimerase" }, "genes": [], "id": "OAIKOHHJFB_1322", "locus": "GCA000209735_01308" }, { "type": "cds", "sequence": "contig_1", "start": 731555, "stop": 731659, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAATTGATAAAAAATATGAATCTGAAATCAAAAAACTTAAACAAGAAGTTTATGAAGCTAATATGTTATTAGTTAAATATGGACTTGTTATTCATACTTGA", "aa": "MAIDKKYESEIKKLKQEVYEANMLLVKYGLVIHT", "aa_hexdigest": "5dc66d56658d75c69e823906de281374", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4026.7597000000005, "isoelectric_point": 8.029940223693849 }, "id": "OAIKOHHJFB_1323", "locus": "GCA000209735_01309" }, { "type": "cds", "sequence": "contig_1", "start": 731764, "stop": 731937, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01261.30" ], "nt": "TTGTATAACGAATTTGAACTTGCTAGAAATCATATAGTAGCTTTCCACTTTAAAGATACTGTACCTGGTAAATTTAGAGATACACCTTTTGGAACTGGCACTGTAGATTTCCCTTATCTTTTAAAAATATTAAAAGATTTAAAAATGAACCAACCTATTATGATAGAAATGTGA", "aa": "MYNEFELARNHIVAFHFKDTVPGKFRDTPFGTGTVDFPYLLKILKDLKMNQPIMIEM", "aa_hexdigest": "4ec6181250e55cf9a0c8a574a353f138", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01261.30", "name": "AP_endonuc_2", "length": 58, "aa_cov": 0.9473684210526315, "hmm_cov": 1.0, "evalue": 1.2241479205251092e-10, "score": 37.551326751708984, "start": 4, "stop": 57 } ], "seq_stats": { "molecular_weight": 6719.8261, "isoelectric_point": 6.707649040222168 }, "id": "OAIKOHHJFB_1324", "locus": "GCA000209735_01310" }, { "type": "cds", "sequence": "contig_1", "start": 731953, "stop": 732078, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGCTTTTGAAATTATGGATACTCAATTCATGGGAACTAATTCAAGAGCAATGACTTATATCAACATGATTAATAGTCCATATTTACAAATCTATCCTGATTTAGGTAATATTTATCAATGA", "aa": "MLAFEIMDTQFMGTNSRAMTYINMINSPYLQIYPDLGNIYQ", "aa_hexdigest": "78a88673ce127e65dc7852d2fbfdc0dc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4810.524100000001, "isoelectric_point": 4.0500284194946286 }, "id": "OAIKOHHJFB_1325", "locus": "GCA000209735_01311" }, { "type": "cds", "sequence": "contig_1", "start": 732109, "stop": 732309, "strand": "-", "frame": 2, "gene": null, "product": "Sugar phosphate isomerase/epimerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A936AG02" ], "nt": "ATGACCCTTAGTGGTCATCGTAAATATCCTTTTGGTTCTAAAGATCCTAAAATCAGAGCTAGAGCCAAAGAAATAATGACTAAAGCAATTATTTTAGCCAAAAAATTAGGTATTAGAACTGTTCAACTTGCTGGTTATGATGTTTATTATGAACCAGGTGATGAAGAAACAAGAAAAAACTTTATTGAAGGAATGAAATGA", "aa": "MTLSGHRKYPFGSKDPKIRARAKEIMTKAIILAKKLGIRTVQLAGYDVYYEPGDEETRKNFIEGMK", "aa_hexdigest": "2f1942fdec59377ad574e7a8d649bca4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A936AG02", "query_cov": 1.0, "subject_cov": 0.8048780487804879, "identity": 0.606, "score": 85.9, "evalue": 1.11e-20, "valid": false, "product": "Sugar phosphate isomerase/epimerase", "uniref50_id": "UniRef50_A0A936AG02" }, "pscc": { "uniref50_id": "UniRef50_A0A936AG02", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A936AG02" ], "product": "Sugar phosphate isomerase/epimerase" }, "genes": [], "id": "OAIKOHHJFB_1326", "locus": "GCA000209735_01312" }, { "type": "cds", "sequence": "contig_1", "start": 732373, "stop": 732537, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAAATATTGAAAATAAAAAAAGACTTCTAGGCATCTATGAAAAAGCTATAAACAACAAATTTAGCTTAGAAGAAAAAATTATAATTGCTAAAGAAGCTGGTTATGACTTTATGGAATTTTCAGTTGATGAAAGTCCTCAAAGATTAGCTCGCTTAGACTGA", "aa": "MPNIENKKRLLGIYEKAINNKFSLEEKIIIAKEAGYDFMEFSVDESPQRLARLD", "aa_hexdigest": "89188bb3d15a1fee9f6ef502f4c23430", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6317.206900000001, "isoelectric_point": 5.368465614318848 }, "id": "OAIKOHHJFB_1327", "locus": "GCA000209735_01313" }, { "type": "cds", "sequence": "contig_1", "start": 732812, "stop": 733171, "strand": "-", "frame": 1, "gene": null, "product": "orotidine 5'-phosphate decarboxylase / HUMPS family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B8401" ], "nt": "ATGAAACCAATGTTACAAATTGCTTTAGATAACTTAACAATTGAAGATGCAATTGCAAGTGCCAAAAAGGTTGAAAAATACATAGACATTATTGAAGTAGGAACTATTCTTATTGCTTCAGAAGGTAAAAAAGCTATTAAAGCTTTGAGAGAAGCATTTCCTACAAAAATCATTGTTGCTGATGGCAAAATTGCAGATGCTGGGAAAGTTTTTGGAAAAATGTTTTTCGAAAATGGTGCTGACTTTACAACATGCATTTGTGCAGCTGAAGTGCCTACAATTGTTGAAACAATGAATATAGCCAAAGAATATGGCAAAAATAAAGAAGTTCAAATTGAATTAACTTCAAACTTTACTTGA", "aa": "MKPMLQIALDNLTIEDAIASAKKVEKYIDIIEVGTILIASEGKKAIKALREAFPTKIIVADGKIADAGKVFGKMFFENGADFTTCICAAEVPTIVETMNIAKEYGKNKEVQIELTSNFT", "aa_hexdigest": "c95f416a38be741e8cd606a348554817", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI00387B8401", "query_cov": 0.865546218487395, "subject_cov": 1.0, "identity": 0.728, "score": 146.0, "evalue": 1.45e-43, "valid": false, "product": "orotidine 5'-phosphate decarboxylase / HUMPS family protein", "uniref50_id": "UniRef50_UPI00387B8401" }, "pscc": { "uniref50_id": "UniRef50_UPI00387B8401", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B8401" ], "product": "orotidine 5'-phosphate decarboxylase / HUMPS family protein" }, "genes": [], "id": "OAIKOHHJFB_1328", "locus": "GCA000209735_01314" }, { "type": "cds", "sequence": "contig_1", "start": 733180, "stop": 733458, "strand": "-", "frame": 2, "gene": null, "product": "PTS sugar transporter subunit IIA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FEFC0FB" ], "nt": "ATGCCACATGCTTCAGCAACAAAAGACGCTGTTTTTGGAAACGGATTCTCACTTATTACTCTTAAAAAACCTATCAAATTTGAAGGAGATGAAAGAGAAGTAAAAATCCTTATGGGACTAGCCGCAAAAGATGGTGAAACTCATACATCTGTTGCTATTCCACAAATTATTGCTGTTTTTGAAGATCCACAAAATATTGAAAAAATAGCACAAGCTAAAACAAAAGAAGAAATTATAAATATCATAAAAGGTGTTGATTATACTAAATACCTTAGTTAG", "aa": "MPHASATKDAVFGNGFSLITLKKPIKFEGDEREVKILMGLAAKDGETHTSVAIPQIIAVFEDPQNIEKIAQAKTKEEIINIIKGVDYTKYLS", "aa_hexdigest": "78542f5ce103e681644f869f4883fd5a", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI002FEFC0FB", "query_cov": 0.967391304347826, "subject_cov": 0.8557692307692307, "identity": 0.596, "score": 103.0, "evalue": 8.23e-27, "valid": false, "product": "PTS sugar transporter subunit IIA", "uniref50_id": "UniRef50_UPI002FEFC0FB" }, "pscc": { "uniref50_id": "UniRef50_UPI002FEFC0FB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FEFC0FB" ], "product": "PTS sugar transporter subunit IIA" }, "genes": [], "id": "OAIKOHHJFB_1329", "locus": "GCA000209735_01315" }, { "type": "cds", "sequence": "contig_1", "start": 733668, "stop": 733946, "strand": "-", "frame": 3, "gene": null, "product": "PTS EIIB type-2 domain-containing protein", "db_xrefs": [ "RefSeq:WP_041594238.1", "SO:0001217", "UniParc:UPI0002EBAD74", "UniRef:UniRef100_C4XFJ9", "UniRef:UniRef50_A0A0D5ZIZ4", "UniRef:UniRef90_C4XFJ9" ], "nt": "ATGAAAGTATTATGTGTTTGTGGCTCAGGTATGGGCACAAGTATGATTATTAAAATGAAAGTTCAACAAGCTATGAAAGAACTTTCAATTCCAGGAACAGTTGAAGCTTTAGGTTTAGGTCAAGGCAAAACAGTTGTAAACAACTTTGATGTTGTTTTGTGTACCTCAAACTTTGTTTCAGAAGTATCAAAAGGCAAAGCAAAAGCTTATGGTTTAAAAAACATCATGGATGTTAATGAAATCAAAGCTGCTCTAAAAGATGCATCAAGCAAGATGTAA", "aa": "MKVLCVCGSGMGTSMIIKMKVQQAMKELSIPGTVEALGLGQGKTVVNNFDVVLCTSNFVSEVSKGKAKAYGLKNIMDVNEIKAALKDASSKM", "aa_hexdigest": "a8172f4c7a6c8a860341a396a2478c4b", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0002EBAD74", "ncbi_nrp_id": "WP_041594238.1", "uniref100_id": "UniRef100_C4XFJ9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002EBAD74", "RefSeq:WP_041594238.1", "UniRef:UniRef100_C4XFJ9" ] }, "ips": { "uniref100_id": "UniRef100_C4XFJ9", "uniref90_id": "UniRef90_C4XFJ9" }, "psc": { "uniref90_id": "UniRef90_C4XFJ9", "product": "PTS EIIB type-2 domain-containing protein", "uniref50_id": "UniRef50_A0A0D5ZIZ4" }, "pscc": { "uniref50_id": "UniRef50_A0A0D5ZIZ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0D5ZIZ4" ], "product": "PTS sucrose transporter subunit IIABC" }, "genes": [], "id": "OAIKOHHJFB_1330", "locus": "GCA000209735_01316" }, { "type": "cds", "sequence": "contig_1", "start": 734045, "stop": 734218, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTTTGCTGCTCAATTCTTTGACTCACACAGACAACACAAACCTACAGTATTCCAAAGAATGCTTAAAGTTAAAGTCGAAACTTTAGAAGATCAAAAGAAAGCTGAAGAAGTTGCTAATGCACCAGTTGAAGAAGTTCCTCAACCAGAAGTTGCAGAACAAGCTGCTTAA", "aa": "MIFAAQFFDSHRQHKPTVFQRMLKVKVETLEDQKKAEEVANAPVEEVPQPEVAEQAA", "aa_hexdigest": "9ef2f2516a6357660ae7dc9281ae3a0a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6522.337500000003, "isoelectric_point": 5.218922233581542 }, "id": "OAIKOHHJFB_1331", "locus": "GCA000209735_01317" }, { "type": "cds", "sequence": "contig_1", "start": 734303, "stop": 734428, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATACTTATCAAGATATGTTTCAGACCAAATAGCTAAAGCTAAAGATATGAAAGAAATCTTGAAATTATCTTCATTCCAAAGAACACCATTCTTAACAGGATTTAATGGTATGTTTGACTGA", "aa": "MQYLSRYVSDQIAKAKDMKEILKLSSFQRTPFLTGFNGMFD", "aa_hexdigest": "8a8e49f17da4a40e9d73196cca569844", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4806.539099999999, "isoelectric_point": 9.301579093933103 }, "id": "OAIKOHHJFB_1332", "locus": "GCA000209735_01318" }, { "type": "cds", "sequence": "contig_1", "start": 734474, "stop": 735097, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGCTGGTATTCGTATGTTCGTTTCAGAACTTCAACAAGCTTTTCAAGGAATTAGTGAAAAATTAGTTCCAGGATCAGTTGTTGCTGTTGACGTTGCTGCCACATATGGTTTCAGCCAAAACTCAGTTACCTATGGTTTCTTATTTGGTTCAATTGGCCAATTCTTAGCTATGGGTCTTGTAATTGGATTAGCTCAAATTCCAAACTCAAGATTTGAAATTGTTATGGTAGTTCCATTATTCATTACATTGTTCTTTAACTCAGGTTCAATTGGAGTATTTGCAAATGCTTCAGGTGGATATAAAGCTACTATTGCAGTACCAATGATCTTTGGTTTCGTTGAAATTATTGTTGTTGCTCTTGCTTTAAGTATGATTAAAAACTTTGGTGTAATGCACCAAGTAGATCTTGACTTCCAATACTTTGAAAAAACTCTCACAAAAGCAGATGCATTCAAATCATTTACAGATTCATTAAATGCAAATTCTGCTAACTTGTTTATTCAAGGTGATGCTACTAAAGCTGTTTCAGCAGCACAATTAAATGATGCTATTACAAACATTAACAGTCAAGATTCATTTAAAATCTTAAAAAGCTTAAGTGAAGTTACAACAAACTGA", "aa": "MQAGIRMFVSELQQAFQGISEKLVPGSVVAVDVAATYGFSQNSVTYGFLFGSIGQFLAMGLVIGLAQIPNSRFEIVMVVPLFITLFFNSGSIGVFANASGGYKATIAVPMIFGFVEIIVVALALSMIKNFGVMHQVDLDFQYFEKTLTKADAFKSFTDSLNANSANLFIQGDATKAVSAAQLNDAITNINSQDSFKILKSLSEVTTN", "aa_hexdigest": "658b8435876284eae9cab90838aaf212", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22199.367700000003, "isoelectric_point": 5.01293773651123 }, "id": "OAIKOHHJFB_1333", "locus": "GCA000209735_01319" }, { "type": "cds", "sequence": "contig_1", "start": 735176, "stop": 735418, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX3F0H9" ], "nt": "ATGTTCGGTATTGCTATTGCATACCAAATTGGTAAATTCTTCGGTAAAAAAGAAGATTCAGCAGAAACTAAAAAACTTTCAAACAAAGTTAAATTATTAGAAGACAATATCTTCACACAAAGTGTATTGATCTTATTAATTTTCTTAATCCTTATCTTAATTATTCAATTCGCACCTGCAGGTGCATCAGTAAGATTTGGTTTAAGCAATGGACTTGTAAACAAAGCAATTTATGGTTCATGA", "aa": "MFGIAIAYQIGKFFGKKEDSAETKKLSNKVKLLEDNIFTQSVLILLIFLILILIIQFAPAGASVRFGLSNGLVNKAIYGS", "aa_hexdigest": "febae287c0e4d5a8ae6e901578a2b714", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00387B4D71", "query_cov": 0.8125, "subject_cov": 0.9848484848484849, "identity": 0.585, "score": 75.1, "evalue": 2.35e-16, "valid": false, "product": "PTS transporter subunit IIC", "uniref50_id": "UniRef50_A0AAX3F0H9" }, "pscc": { "uniref50_id": "UniRef50_A0AAX3F0H9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAX3F0H9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8750.335599999991, "isoelectric_point": 9.631657218933103 }, "id": "OAIKOHHJFB_1334", "locus": "GCA000209735_01320" }, { "type": "cds", "sequence": "contig_1", "start": 735497, "stop": 735661, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTACAGGACACGTTATGTTCCAACAAGCAGCTGTTGTTGTTCCAGTTGTACTTGTATTAATGTTCTCAGGACAACAATTTAGAACAGCAGAAGGTGCTATTAATGTAGGCGGCCAAGTCGGTACAATCTTCTTCTCAGGTTTATTACTTGGTACTTACTGA", "aa": "MITGHVMFQQAAVVVPVVLVLMFSGQQFRTAEGAINVGGQVGTIFFSGLLLGTY", "aa_hexdigest": "238b9252c55a56c5456c2006f18af612", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5701.680299999998, "isoelectric_point": 6.50155086517334 }, "id": "OAIKOHHJFB_1335", "locus": "GCA000209735_01321" }, { "type": "cds", "sequence": "contig_1", "start": 735743, "stop": 735916, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGGTGTCTTAATGATGCAAATCGGTTCAGGAATGATTGTTGGTTTAGCTAAACCAGTGTTCCTTGCATTCTCTAAATTCGGTAAAGGGCTTACACCACTTGATACATACTTAGGACAAGTTTCAGTAGAAAACTTCCTTAACTCAGTTGGTCAAGGTTTTGCTTCATGA", "aa": "MIGVLMMQIGSGMIVGLAKPVFLAFSKFGKGLTPLDTYLGQVSVENFLNSVGQGFAS", "aa_hexdigest": "bf903f80e1dca179388b132ae892eb90", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5968.034700000002, "isoelectric_point": 8.189692878723147 }, "id": "OAIKOHHJFB_1336", "locus": "GCA000209735_01322" }, { "type": "cds", "sequence": "contig_1", "start": 736335, "stop": 737030, "strand": "-", "frame": 3, "gene": null, "product": "phospho-furanose lactonase", "db_xrefs": [ "EC:3.1.1.25", "GO:0050490", "SO:0001217", "UniRef:UniRef50_Q4A724", "UniRef:UniRef90_C4XFK1" ], "nt": "ATGTGTGTCGCAGAAATTGAAGAAGGTATGGACGTTTACAACTATAATGGACCAGTTGTAAAACGTGGTAAAGCTAAAGCTGGTATTATTAAAGCTGGTACAGGTTATGCTGCTATTGACCGTCTTGAATTAAAAGCACTTGAAGTTGCTGCTAGAACCTCAATTACAACAGGTTGCCCAATTTTAGTACATACTCAATTAGGTACAATGGCTTTAGAAGTTGCACAACACTTAATTGACTTTGGTGCAAACCCTCGTAAAATTCAAATTTCTCACTTAAATAAAAACCCAGACCCATACTACTATGAAAAAGTTATTAAAGATACAGGCGTTACACTTTGTCTTGATGGACCAGACAGAGTTAAATACTACCCTGACTCAACTCTTGCAGATAACATTAAATACTTAGTAGACCAAGGTCTTCAAAAACATATAACATTAAGCCTTGATGCTGGTAGAATTCTATACCAACGTAACTATGGTTTAACAAAAGGTAAAGAAACATTTGGATTCTCATACTTATTTGATAGATTTATTCCTTTACTTAAAAAAGTTGGTGTTCCTCAAGATGCTATTGATGACATTTTGATCAACAACCCACGTGAAATTCTTGCATTTGATGAACCTCGTAAATATGAACCATCAAAAGTATCAAACGAATTAAAACAACTTAAAAAAAATCTAAAACTAGATTAA", "aa": "MCVAEIEEGMDVYNYNGPVVKRGKAKAGIIKAGTGYAAIDRLELKALEVAARTSITTGCPILVHTQLGTMALEVAQHLIDFGANPRKIQISHLNKNPDPYYYEKVIKDTGVTLCLDGPDRVKYYPDSTLADNIKYLVDQGLQKHITLSLDAGRILYQRNYGLTKGKETFGFSYLFDRFIPLLKKVGVPQDAIDDILINNPREILAFDEPRKYEPSKVSNELKQLKKNLKLD", "aa_hexdigest": "3645091e774248648b79842571d6af99", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 736335, "stop": 737330, "observations": { "mutation": [ 737066 ], "stop": [ 737066 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFK1", "query_cov": 1.0, "subject_cov": 0.6434540389972145, "identity": 1.0, "score": 464.0, "evalue": 2.65e-163, "gene_start": 1, "gene_end": 231, "reference_start": 129, "reference_end": 359, "reference_sequence": "MQKRRFMKKEKFVRTVLGDVPASSIGITDCHDHLIKNGGPEMEEHIDFLMIDVEAAKKELQEFVDHGGKTMVTMDPPNVGRDVFRMMEIANAFKGKANIIMSTGFHKAKFYDKYCSWLANVPTDDIVKMCVAEIEEGMDVYNYNGPVVKRGKAKAGIIKAGTGYAAIDRLELKALEVAARTSITTGCPILVHTQLGTMALEVAQHLIDFGANPRKIQISHLNKNPDPYYYEKVIKDTGVTLCLDGPDRVKYYPDSTLADNIKYLVDQGLQKHITLSLDAGRILYQRNYGLTKGKETFGFSYLFDRFIPLLKKVGVPQDAIDDILINNPREILAFDEPRKYEPSKVSNELKQLKKNLKLD" }, "paralog": false, "identity": 0.9969788519637462, "score": 669.0, "evalue": 1.15e-246, "description": "Internal stop codon at 737066. Nonsense mutation around 737066.", "psc": { "uniref90_id": "UniRef90_C4XFK1", "product": "phospho-furanose lactonase", "ec_ids": [ "3.1.1.25" ], "uniref50_id": "UniRef50_Q4A724", "go_ids": [ "GO:0050490" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFK1", "EC:3.1.1.25", "UniRef:UniRef50_Q4A724", "GO:0050490" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A724", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A724" ], "product": "Phospho-furanose lactonase" } }, "id": "OAIKOHHJFB_1337", "locus": "GCA000209735_01323" }, { "type": "cds", "sequence": "contig_1", "start": 737064, "stop": 737396, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02126.24" ], "nt": "ATGAAGAAAGAAAAATTTGTTAGGACTGTGCTAGGCGATGTGCCTGCTAGTTCTATCGGTATTACTGACTGTCACGACCACCTTATAAAGAACGGTGGCCCTGAAATGGAAGAACACATTGACTTTTTAATGATCGATGTTGAAGCTGCTAAAAAAGAACTTCAAGAATTCGTTGATCACGGTGGAAAAACAATGGTTACAATGGATCCACCTAATGTTGGACGTGATGTATTTAGAATGATGGAAATTGCTAATGCATTCAAAGGCAAAGCAAACATTATTATGTCTACAGGTTTCCACAAAGCTAAATTCTATGACAAATACTGTTCATGA", "aa": "MKKEKFVRTVLGDVPASSIGITDCHDHLIKNGGPEMEEHIDFLMIDVEAAKKELQEFVDHGGKTMVTMDPPNVGRDVFRMMEIANAFKGKANIIMSTGFHKAKFYDKYCS", "aa_hexdigest": "14467491478e05d57b812bd0a5b99b68", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02126.24", "name": "PTE", "length": 92, "aa_cov": 0.8090909090909091, "hmm_cov": 0.9782608695652174, "evalue": 2.861295450370424e-07, "score": 26.286685943603516, "start": 17, "stop": 105 } ], "seq_stats": { "molecular_weight": 12380.227199999987, "isoelectric_point": 6.1835935592651365 }, "id": "OAIKOHHJFB_1338", "locus": "GCA000209735_01324" }, { "type": "cds", "sequence": "contig_1", "start": 738735, "stop": 739613, "strand": "-", "frame": 3, "gene": null, "product": "Lysine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5HK23" ], "nt": "TTGCATGATTATCTTTCAGGAGCAGCTGCTAAACCATTTACTACACATCATAATTCATTGAACCAAGATTTTGTTTTACGAATAGCAACAGAAATTCCGCTTAAAAAATTAGTTGTTGGTGGATTTGAAAGAGTTTATGAAATGGGTCGCATATTCAGAAATGAAGGATACGACACAACACATAACCCTGAATTTACAACTATTGAATTTTATGAAGCTTATTCAAATGTTGAAGGTATGATGAATAGAACTGAAACATTAATTAAAGAACTTTGCAAAAAAATTGGTAAAAGTAAATTTGTAACAAATGGTGTTGAAGTTGATTTATCAAAACCATTTAAGCGTGTAAATATGATTGATGAAGTAAGCAAGAAAACTGGTAAAAACTTTAGAAAAATTACTTTAGAAGAAGCTATTGAAGTAGCTAAAGCTTACAAAATCAAAATCGAAAAATTCTTTACTATTGGACACATTATTAATGCTTTATATGAAGAATTAGTTGAACCAACATTAATTCAACCTACATTCTTATATGGACATCCAATTGAAATTTCTCCTTTAAGTGCTAAGAGTGATGATCCAAGATTTACTGAACGTGCTGAATTATTTATTAACACAAAAGAATATGCCAACATGTATACAGAATTAAGTGATCCAATTGACCAATTAGAAAGATTCGAAAGTCAATTAGAAGAAAAGAATAAAGGTAATGATGAAGCAAGTGATATTGACTATGACTTTGTTGATGCTTTAGAATATGGCATGCCTCCAACAGGTGGTTGTGGAATTGGAATTGACCGTTTAGTTATGTTATTAACTGAAACAGATTCTATTAGAGATGTACTTTTATTTCCCACACTAAAAAGAATCAAAAAATAA", "aa": "MHDYLSGAAAKPFTTHHNSLNQDFVLRIATEIPLKKLVVGGFERVYEMGRIFRNEGYDTTHNPEFTTIEFYEAYSNVEGMMNRTETLIKELCKKIGKSKFVTNGVEVDLSKPFKRVNMIDEVSKKTGKNFRKITLEEAIEVAKAYKIKIEKFFTIGHIINALYEELVEPTLIQPTFLYGHPIEISPLSAKSDDPRFTERAELFINTKEYANMYTELSDPIDQLERFESQLEEKNKGNDEASDIDYDFVDALEYGMPPTGGCGIGIDRLVMLLTETDSIRDVLLFPTLKRIKK", "aa_hexdigest": "34de8cf3f4ef36d74884392ef4f02aaa", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0030049D44", "query_cov": 0.9794520547945206, "subject_cov": 0.9225806451612903, "identity": 0.6709999999999999, "score": 385.0, "evalue": 5.81e-132, "valid": false, "gene": "lysS", "product": "lysine--tRNA ligase", "ec_ids": [ "6.1.1.6" ], "uniref50_id": "UniRef50_A0A1V5HK23", "kegg_orthology_id": "K04567", "go_ids": [ "GO:0004824", "GO:0005524", "GO:0005737", "GO:0006430" ] }, "pscc": { "uniref50_id": "UniRef50_A0A1V5HK23", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1V5HK23" ], "product": "Lysine--tRNA ligase" }, "genes": [], "id": "OAIKOHHJFB_1339", "locus": "GCA000209735_01325" }, { "type": "cds", "sequence": "contig_1", "start": 739665, "stop": 739859, "strand": "-", "frame": 3, "gene": null, "product": "amino acid--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00257604EB" ], "nt": "ATGAAAACTCATACAGGTGAAATGACAGTTAGGGCTCAAGATATTCAATTATTAACTAAAGCTCTTAAACCCCTTCCAGATAAATTTCATGGATTAACTGATACTGAAGAAAGATATCGTCACAGATACTTAGATTTAATTACTAATCCAGAATCAAGAAATACATTCATTATGAGAACAAAAATTGTTCAATGA", "aa": "MKTHTGEMTVRAQDIQLLTKALKPLPDKFHGLTDTEERYRHRYLDLITNPESRNTFIMRTKIVQ", "aa_hexdigest": "6f306cee6dd0378974b3e25ee4b1629a", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI00257604EB", "query_cov": 0.96875, "subject_cov": 0.8378378378378378, "identity": 0.629, "score": 85.9, "evalue": 8.26e-21, "valid": false, "product": "amino acid--tRNA ligase-related protein", "uniref50_id": "UniRef50_UPI00257604EB" }, "pscc": { "uniref50_id": "UniRef50_UPI00257604EB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00257604EB" ], "product": "amino acid--tRNA ligase-related protein" }, "genes": [], "id": "OAIKOHHJFB_1340", "locus": "GCA000209735_01326" }, { "type": "cds", "sequence": "contig_1", "start": 739875, "stop": 740201, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAATACACAGAACAAGAACAAATTCGTCGTGATAAACTAGAATTTTATAAAAAATTTGATGTAGTACCTTTTAAAAAAGCTTATGGTCTAGGTAAATTGAGTACAAGTGATGAACTTAATAAAAAATATGGTCAATTTGCTAGAGAAGAATTAGAAGCTAAAAAAGTTAAGAAAAATATTAGTGGTAGATTAATTACAGCTAGAGGACCTTTCTTAGTATTAAAAGATCGTAAAGGAACAATTCAAGTTTATTTCAATAAAAAAGAAAATCCTGAATTAACTAAAATAGTTGAAACATTTGACTTAGGGGACATTTTATGA", "aa": "MTKYTEQEQIRRDKLEFYKKFDVVPFKKAYGLGKLSTSDELNKKYGQFAREELEAKKVKKNISGRLITARGPFLVLKDRKGTIQVYFNKKENPELTKIVETFDLGDIL", "aa_hexdigest": "80f2fa5d07f87b0faa615d1d6f0e3697", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12652.541799999983, "isoelectric_point": 9.638877677917481 }, "id": "OAIKOHHJFB_1341", "locus": "GCA000209735_01327" }, { "type": "cds", "sequence": "contig_1", "start": 740319, "stop": 740510, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XAM5", "UniRef:UniRef90_A0AB32XAM5", "PFAM:PF13518.12" ], "nt": "ATGCTTGATCAAAAGATAAAAATTGTCGAATTATATCTTCGAGAATTTAGTATTTTAGAAATATCTAAAATAATGAATAACTCTTATTCAACATGCTATTCAGTGATAGAGAAATACAAAAAGTTTGGTTATAATTCTTTTGCTATGGAAAAGAAAAAAGGAAGAAAATCTAAAATGAATTTAAAGTTATAA", "aa": "MLDQKIKIVELYLREFSILEISKIMNNSYSTCYSVIEKYKKFGYNSFAMEKKKGRKSKMNLKL", "aa_hexdigest": "abe104260aa19d2c84c2d30157ba603a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 740319, "stop": 740597, "observations": { "mutation": [ 740543 ], "stop": [ 740543 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XAM5", "query_cov": 1.0, "subject_cov": 0.6847826086956522, "identity": 1.0, "score": 119.0, "evalue": 1.01e-33, "gene_start": 1, "gene_end": 63, "reference_start": 30, "reference_end": 92, "reference_sequence": "MESDSFLCNSNLKKKKNKWNREIKGYLKLMLDQKIKIVELYLREFSILEISKIMNNSYSTCYSVIEKYKKFGYNSFAMEKKKGRKSKMNLKL" }, "paralog": false, "identity": 0.9782608695652174, "score": 179.0, "evalue": 3.38e-60, "description": "Internal stop codon at 740543. Nonsense mutation around 740543.", "psc": { "uniref90_id": "UniRef90_A0AB32XAM5", "uniref50_id": "UniRef50_A0AB32XAM5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XAM5", "UniRef:UniRef50_A0AB32XAM5" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB32XAM5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XAM5" ] } }, "hypothetical": true, "pfams": [ { "id": "PF13518.12", "name": "HTH_28", "length": 41, "aa_cov": 0.6507936507936508, "hmm_cov": 1.0, "evalue": 1.2154330179902854e-07, "score": 27.81732177734375, "start": 4, "stop": 44 } ], "seq_stats": { "molecular_weight": 7550.944300000001, "isoelectric_point": 9.752406501770018 }, "id": "OAIKOHHJFB_1342", "locus": "GCA000209735_01328" }, { "type": "tRNA", "sequence": "contig_1", "start": 740933, "stop": 741009, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(tct)", "amino_acid": "Arg", "anti_codon": "tct", "score": 64.3, "nt": "GGTCGCGTAGCTCAGTAGGATAGAGCACGAGCCTTCTAAGCCCGTGGTCAGAGGTTCGAATCCTCTCGTGATCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 740967, 740969 ], "id": "OAIKOHHJFB_14", "locus": "GCA000209735_01329" }, { "type": "tRNA", "sequence": "contig_1", "start": 741048, "stop": 741125, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(cct)", "amino_acid": "Arg", "anti_codon": "cct", "score": 67.5, "nt": "GTCCTTGTAGCTCAGATGGATCAGAGCACGAGCCTCCTAAGCTTGGGGTGGGTGGTTCAATTCCACTCAAGGACGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 741050, 741052 ], "id": "OAIKOHHJFB_15", "locus": "GCA000209735_01330" }, { "type": "cds", "sequence": "contig_1", "start": 741208, "stop": 741687, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFK4", "UniRef:UniRef90_C4XFK4" ], "nt": "ATGAATAAAAAATTTTTAAAATTAGGCTCAATTGCAGGAATTTTATCTTTTGCACCAGTAGCAATTAGTGCTGGATGTGGTTCTAAAGGTGGAGAAACCCCTACTGATCCAGTTGCTGCTAAGAAAGCATTAGTTGAACAAGCATTAAAAGATTTAAATGCTAAAATTGAAACTGTTACTGATGAAACTAAAAAAGCTGAACTTAAAAAGGAAGCAGAAGCTATTAAAAAAGATTTCGATGCTGCTAAAACAGTTAAAGATTTTGAAGCTGTAGATGCAAAAATTAAAAAAGTTGTTGCTAAGGTTGAAAGTAAAAGTACATCTGACCAAGACAAATATAAAACATGCTTTACTTTAGGAGAAGGCAAAGATAGAATTAAAGAAGCTATTGAAGCCATAAAAAAAGATTCTAAATCATATTCATTTGCATATGATCGTAAAGGCCACAACATTGTTTGCTTTAAAGGAAGAATTAATTGA", "aa": "MNKKFLKLGSIAGILSFAPVAISAGCGSKGGETPTDPVAAKKALVEQALKDLNAKIETVTDETKKAELKKEAEAIKKDFDAAKTVKDFEAVDAKIKKVVAKVESKSTSDQDKYKTCFTLGEGKDRIKEAIEAIKKDSKSYSFAYDRKGHNIVCFKGRIN", "aa_hexdigest": "2e5a928523326b37f2104c3112e32e56", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 741181, "stop": 741942, "observations": { "mutation": [ 741685 ], "stop": [ 741685 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFK4", "query_cov": 1.0, "subject_cov": 0.6284584980237155, "identity": 1.0, "score": 297.0, "evalue": 2.04e-100, "gene_start": 1, "gene_end": 159, "reference_start": 10, "reference_end": 168, "reference_sequence": "MVKIMRRKSMNKKFLKLGSIAGILSFAPVAISAGCGSKGGETPTDPVAAKKALVEQALKDLNAKIETVTDETKKAELKKEAEAIKKDFDAAKTVKDFEAVDAKIKKVVAKVESKSTSDQDKYKTCFTLGEGKDRIKEAIEAIKKDSKSYSFAYDRKGHNIVCFKGRINWSNGDKVIALNITGALKEHIEKNSVQLANAKKPTYNSKKGESISSILDFKLEGNTVKFSFKLAKFENNKPVLDDPIVYNAEVSLA" }, "paralog": false, "identity": 0.9920948616600791, "score": 486.0, "evalue": 4.26e-177, "description": "Internal stop codon at 741685. Nonsense mutation around 741685.", "psc": { "uniref90_id": "UniRef90_C4XFK4", "uniref50_id": "UniRef50_C4XFK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFK4", "UniRef:UniRef50_C4XFK4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFK4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17277.756299999994, "isoelectric_point": 9.341291618347167 }, "id": "OAIKOHHJFB_1343", "locus": "GCA000209735_01331" }, { "type": "cds", "sequence": "contig_1", "start": 742002, "stop": 742127, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGATGAAGAAACCAAAAAAGAAATGATAAAATTATCTCTTTTCTTTGGTTTCAAATTAGCATATGATTATATTTATTGTGTACTTTATTTAATTAGATTATTGTCAAATATGAAAAATTAA", "aa": "MDDEETKKEMIKLSLFFGFKLAYDYIYCVLYLIRLLSNMKN", "aa_hexdigest": "54551c09f10e2b39f7a050e4f48a271d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5000.9117, "isoelectric_point": 6.057411003112793 }, "id": "OAIKOHHJFB_1344", "locus": "GCA000209735_01332" }, { "type": "cds", "sequence": "contig_1", "start": 742398, "stop": 742520, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTAGGACCTAGAATGCATATAGGTGTATCCATACCTATGGTTATGATCATAATGATGGGATTTGGTCTATTTGCGCTTTCTATTCCTCTTTACTATGCAAAAGAAACCATGGTTTGA", "aa": "MFLGPRMHIGVSIPMVMIIMMGFGLFALSIPLYYAKETMV", "aa_hexdigest": "cfb0ab3d9054e9ace7fcd6ff0300b101", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4507.642900000001, "isoelectric_point": 8.274855613708496 }, "id": "OAIKOHHJFB_1345", "locus": "GCA000209735_01333" }, { "type": "tRNA", "sequence": "contig_1", "start": 742825, "stop": 742899, "strand": "-", "gene": "trnQ", "product": "tRNA-Gln(ttg)", "amino_acid": "Gln", "anti_codon": "ttg", "score": 64.1, "nt": "TGCCCCATAGCCAAGCGGTAAGGCAACGGGTTTTGGTTCCGTCATGCGTTAGTTCGAATCTAACTGGGGCAACCA", "db_xrefs": [ "SO:0000261" ], "anti_codon_pos": [ 742865, 742867 ], "id": "OAIKOHHJFB_22", "locus": "GCA000209735_01334" }, { "type": "tRNA", "sequence": "contig_1", "start": 742903, "stop": 742986, "strand": "-", "gene": "trnY", "product": "tRNA-Tyr(gta)", "amino_acid": "Tyr", "anti_codon": "gta", "score": 70.9, "nt": "GGAGGGGTAGCGAAGCGGCCAAACGCGGGTGGCTGTAACCCACTTCCTCACGGTTCGGGGGTTCGAATCCCTCCCCCTCCACCA", "db_xrefs": [ "SO:0000272" ], "anti_codon_pos": [ 742978, 742980 ], "id": "OAIKOHHJFB_21", "locus": "GCA000209735_01335" }, { "type": "cds", "sequence": "contig_1", "start": 743277, "stop": 743867, "strand": "+", "frame": 3, "gene": null, "product": "DNA methylase adenine-specific domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8L726" ], "nt": "ATGTTTGGTTCACTAACTGAAAGAAACATAAAAGATATCATCAAAAAAGAAAAATTAACAGATGTTAAATATTTAATTAAATATATTGAAAAAAATCCAAAACTTATGGATGTTAGATCAATGTACCAAGTAGCTCAATATGCAAACTGCTTGAGATTAGGTCAAAAACCTTTTTATACAGATGAAAGTTTACTAAACTCAATTTACAAAAGACTTCCAAATATTAGAGGCAAAGAATTAACGATTGTTGATCCTTTATGTGGTGTTGGTATTTTCTTACCTTTAATTATTGAAAAATATGGAGACAAAGACAAACTAAGTATTACTTTTTATGATGTAGATAATGAAACAATAAATATTGCTCGCGCTTTATCTAAAATGATAAAACTTAAAGTTACTTTTGGTGTTAGTTTTCAAAATGCTGATTTTCTAATGAAAAGAACTAATGAACCAATTGACTTATTAATTGGTGATTTCCCTAACATAAGAATGTCTCATCCAGCTTTAAGAAAAAAATATCCTTCATTAACTAAAGAAAACAAATTATTACAAAGTTTTGCTCCATTATACTTAATGCAAAGTAAAAAATGA", "aa": "MFGSLTERNIKDIIKKEKLTDVKYLIKYIEKNPKLMDVRSMYQVAQYANCLRLGQKPFYTDESLLNSIYKRLPNIRGKELTIVDPLCGVGIFLPLIIEKYGDKDKLSITFYDVDNETINIARALSKMIKLKVTFGVSFQNADFLMKRTNEPIDLLIGDFPNIRMSHPALRKKYPSLTKENKLLQSFAPLYLMQSKK", "aa_hexdigest": "a4736865b936e010739a379ee8d7e4b1", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A3P8L726", "query_cov": 0.8775510204081632, "subject_cov": 0.9398907103825137, "identity": 0.855, "score": 297.0, "evalue": 1.16e-100, "valid": false, "product": "DNA methylase adenine-specific domain-containing protein", "uniref50_id": "UniRef50_A0A3P8L726" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8L726", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8L726" ], "product": "DNA methylase adenine-specific domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_1346", "locus": "GCA000209735_01336" }, { "type": "cds", "sequence": "contig_1", "start": 744402, "stop": 744719, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACACAAACCAAATTTCGGTTGTTCCTAAACCTGCTAATGTTGCAGTTAGTGACAAACTATACATGCTTGAGCCTTTAACTCAAATTACTGAAAAGGATTTAGAATTCTTTAACTCAAAAGATTTTAGACACTTTTATCAAATAGCCACAAACTTTTCATCAAGATCAAGCTCAGTTGATACATCAAGTGTATTCTACTTCGGTTTGCTTCAAGAAGATGATGTTGAAACAAACAAAAAACAAAGCGAAACTCAAGAATACGATTTCTCAGATGACTTTGATATTGACACCAAAGAATACGAAAGCAGTTTCTAA", "aa": "MNTNQISVVPKPANVAVSDKLYMLEPLTQITEKDLEFFNSKDFRHFYQIATNFSSRSSSVDTSSVFYFGLLQEDDVETNKKQSETQEYDFSDDFDIDTKEYESSF", "aa_hexdigest": "c283a4e7094f8b701bc0531b84b86c42", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12211.143299999992, "isoelectric_point": 4.2429399490356445 }, "id": "OAIKOHHJFB_1347", "locus": "GCA000209735_01337" }, { "type": "cds", "sequence": "contig_1", "start": 745190, "stop": 745507, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTACATTGAAGGACCAGGACATGGTGGCCAAGTTATGACATCTAACTCATACTTAGATGGAAGTTACACAGAATTATTCCCACATGTAACACAAGATGAAGCCGGAATGCAACATTTATTTAAATACTTCTCATTCCCTGGTGGAACAGCTTCACATGCTGCTCCTGAAACCCCTGGATCAATTCATGAAGGTGGAGAATTAGGTTACTCAATTTCACACGCAACAGGTGCAATTTTAGATAACCCTGATGTTATTGCTGCTACTATCGTTGGTGATGGTGAAGCTGAAACAGGACCTCTTGCAACTAGCTGA", "aa": "MFYIEGPGHGGQVMTSNSYLDGSYTELFPHVTQDEAGMQHLFKYFSFPGGTASHAAPETPGSIHEGGELGYSISHATGAILDNPDVIAATIVGDGEAETGPLATS", "aa_hexdigest": "abaf62c10f830fc60a38ce8589f21cd4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10886.74039999999, "isoelectric_point": 4.366280555725097 }, "id": "OAIKOHHJFB_1348", "locus": "GCA000209735_01338" }, { "type": "cds", "sequence": "contig_1", "start": 745688, "stop": 745795, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATGAATTAATGGCAAAAACTTTAGACTCAGTTATTGAAGAAATTCAAAGCATTCAAACAAAAGCTCGTAAAAAACCAGCTGACAAAGCTAAAAGACCAGTATGA", "aa": "MHELMAKTLDSVIEEIQSIQTKARKKPADKAKRPV", "aa_hexdigest": "03a2515dbd7b44d45134cf5fd3f995f6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3991.6814000000013, "isoelectric_point": 9.696447944641115 }, "id": "OAIKOHHJFB_1349", "locus": "GCA000209735_01339" }, { "type": "cds", "sequence": "contig_1", "start": 745744, "stop": 746040, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCCATTCTTCTTTTGCCTTTAGGAGCAATAGCAGCGATTTCTTTTACCAATTTACCTTTTTTATCAAATAATTCTTCAGGACGATATGATCTTAATCATTTTTCAAGTGCATCTGCATGTTCCATGTTTTCTGCATTAATTGGAAGTGGAACTTGGTGTGCACGGAAGCTTCCTTCAATAGCTTCTTTGTTTCATGATTTAGGACCTGTTCAACCTTTAGGTGTTCTAAGAACGATCATTGGTCATACTGGTCTTTTAGCTTTGTCAGCTGGTTTTTTACGAGCTTTTGTTTGA", "aa": "MPILLLPLGAIAAISFTNLPFLSNNSSGRYDLNHFSSASACSMFSALIGSGTWCARKLPSIASLFHDLGPVQPLGVLRTIIGHTGLLALSAGFLRAFV", "aa_hexdigest": "be327aa5fc9f75f675274ea0a8aac226", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10272.94589999999, "isoelectric_point": 9.495048713684085 }, "id": "OAIKOHHJFB_1350", "locus": "GCA000209735_01340" }, { "type": "cds", "sequence": "contig_1", "start": 746147, "stop": 746275, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGAATTAGGAACATACTTTGCTGACTTAGTAAAAAGAAATCCAGAAAACTTTAGAATTTTTGGACCAGATGAAACTAAATCAAACCGTCTTTACAACTTATTCAAAGTTACAAACCGTCAATGA", "aa": "MVELGTYFADLVKRNPENFRIFGPDETKSNRLYNLFKVTNRQ", "aa_hexdigest": "214a769cc8436e2b93a27ee96b92b44a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5022.652500000001, "isoelectric_point": 9.394735908508299 }, "id": "OAIKOHHJFB_1351", "locus": "GCA000209735_01341" }, { "type": "cds", "sequence": "contig_1", "start": 746276, "stop": 746467, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAGAATTGATTCTAAATTAGATGAAGCTCTTAGTCCAGTAGGTAGAATCATTGACTCACAACTTTCAGAACACCAAGCTCAAGGTTTCTTAGAAGGTTATGTATTAACAGGTCGTCATGGTATCTTTGCTTCATATGAATCATTCTTAAGAGTTGTTGACTCAATGGTTACACAACACATGAAATGA", "aa": "MERIDSKLDEALSPVGRIIDSQLSEHQAQGFLEGYVLTGRHGIFASYESFLRVVDSMVTQHMK", "aa_hexdigest": "956d56023b3219d2e85c34ae7d915f9d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7124.998099999999, "isoelectric_point": 5.415187263488768 }, "id": "OAIKOHHJFB_1352", "locus": "GCA000209735_01342" }, { "type": "cds", "sequence": "contig_1", "start": 746522, "stop": 746791, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTACTTCAACTGCTTTCCAACAAGACCACAATGGTTATACACACCAAGACCCAGGTATTATTGGACACATGGCAGATAAAAGACCAGAACTAATTAGAGAATACTTACCAGCTGATACAAACACATTATTAGCCGTTATGGATAAAGCATTTACAGAAAGAAATGTAATTAACTTAATCGTTTCTTCAAAACAACCAAGACACCAATTCTACTCAGTAGAAGAAGCTGAAACATTGGTTGAAAAAGGTTTAGACATTATTGATTGA", "aa": "MATSTAFQQDHNGYTHQDPGIIGHMADKRPELIREYLPADTNTLLAVMDKAFTERNVINLIVSSKQPRHQFYSVEEAETLVEKGLDIID", "aa_hexdigest": "74af9989c280f6b35af6fe415926b4db", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10085.208899999996, "isoelectric_point": 5.021747779846191 }, "id": "OAIKOHHJFB_1353", "locus": "GCA000209735_01343" }, { "type": "cds", "sequence": "contig_1", "start": 746906, "stop": 747310, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTAGATTTGTTAACGTTGTAGATTTACTTAAATTAAGACATCCAAAAATTGACCCACGCGGTTTAAGTGATGAAGAATTTGATGAAATCTTTACAAAAGACAAACCAGTATTATTTGCTTTCCATGGTTTTGAAGGTATTTTAAGAGACATCTTCTTTGACCGTCACAACCACAATTTAATTGCTCACGGTTATAGAGAAAATGGTGACATTACAACATCATTCGACATTCGTCAATTAAGTCACATGGACAGATTCCACATGGCTAGCGATGCTGCTGCAGCTGTATTCGGATCATCAAAAGCAAAAGAATTCATGGACAAAATGGAAGAAACAATCCAATTCCACAACAAATACATTCGTGAAGTTGGTACAGATATTCCTGAAGTTAAAAACTGA", "aa": "MKIRFVNVVDLLKLRHPKIDPRGLSDEEFDEIFTKDKPVLFAFHGFEGILRDIFFDRHNHNLIAHGYRENGDITTSFDIRQLSHMDRFHMASDAAAAVFGSSKAKEFMDKMEETIQFHNKYIREVGTDIPEVKN", "aa_hexdigest": "841dfb96fb36c35c9497b9abd1ba2ec1", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AA37XNB4", "query_cov": 1.0, "subject_cov": 0.8072289156626506, "identity": 0.657, "score": 192.0, "evalue": 2.08e-60, "valid": false, "product": "Xylulose 5-phosphate/Fructose 6-phosphate phosphoketolase C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A3B0NYC0" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15654.597799999987, "isoelectric_point": 6.0416666030883786 }, "id": "OAIKOHHJFB_1354", "locus": "GCA000209735_01344" }, { "type": "cds", "sequence": "contig_1", "start": 747504, "stop": 747671, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAGCTGGTATATTCTTCTTTACAGCTGTGATGGAATTTCTACCTGAGTTTTATCATGCACATCATGACAAAGATTTGTTCAAAAAAGTTATGATTACATTTATGGCTGGACTTGTGGTTTGTGCCTTGATATTAGCATTGCATCAACATGGGACATATAATTAA", "aa": "MIAGIFFFTAVMEFLPEFYHAHHDKDLFKKVMITFMAGLVVCALILALHQHGTYN", "aa_hexdigest": "a8919743e0d3618869803bc5ad767110", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6329.501399999999, "isoelectric_point": 6.476882743835449 }, "id": "OAIKOHHJFB_1355", "locus": "GCA000209735_01345" }, { "type": "cds", "sequence": "contig_1", "start": 747789, "stop": 748160, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAGTTAAAAAGATAAGTGAAAAACATTTAGCTAAAAGTAAAGCAAGTGTATTTGTGCATACACATGATATGGTTCATAACCATGAACATGGTGAAAATGAAGAACATTCTCATGATCATGTGGCACAAGAACATAAAATATCAACAGACGAAAAAAATGCTAATAACAAACTAAAAGTAGTTGCATTATTGTTGTTATTAACTCACAGAATACCAGAAGGTTTCTTAATTGGTTATTCATTAAATAACTTATATAATCAGAATATTGGCTCATTAAGTATTGCATTCTTTGTTTCATTTATAATGCACTTAATACCTGAAGAAATGGTTTTTTATTATAGACAACGTGAAATGGGTGTAGGTCGTTGA", "aa": "MTVKKISEKHLAKSKASVFVHTHDMVHNHEHGENEEHSHDHVAQEHKISTDEKNANNKLKVVALLLLLTHRIPEGFLIGYSLNNLYNQNIGSLSIAFFVSFIMHLIPEEMVFYYRQREMGVGR", "aa_hexdigest": "9c001a56dc7c38a9eb214bd163165308", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14216.102099999991, "isoelectric_point": 6.864524650573731 }, "id": "OAIKOHHJFB_1356", "locus": "GCA000209735_01346" }, { "type": "cds", "sequence": "contig_1", "start": 748236, "stop": 748544, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAAATTTATTAGAGCTCTTGAAAATGGTGCTTTAACAATAGGCACAAGAGAAATAGCAGCTAAATTCTATTTGATCTTAATTGCATTTGCAATTATATTTTTAGTGCCAGTTTTAGTGGCATTAATTTTTCCTTTATTTAAGAAAAGCTTAAGTAAAAAAGGAAGTATATTGCTTTATGCTTTTGTAACTGGTTTCTTTTTAACGATGGCTTTATTTGGATTTTTAAAAGAAGCTTTGGAAATAAGTTCAATTAGTGCTCCGGCATCAATGCCACCAGAAGCTTCGTCAGCGCAGATTAAATGA", "aa": "MRKFIRALENGALTIGTREIAAKFYLILIAFAIIFLVPVLVALIFPLFKKSLSKKGSILLYAFVTGFFLTMALFGFLKEALEISSISAPASMPPEASSAQIK", "aa_hexdigest": "89fbce365ff8e912c1f7d7670d63a162", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11144.412399999987, "isoelectric_point": 9.995065498352051 }, "id": "OAIKOHHJFB_1357", "locus": "GCA000209735_01347" }, { "type": "cds", "sequence": "contig_1", "start": 748577, "stop": 749233, "strand": "-", "frame": 1, "gene": null, "product": "YebC/PmpR family DNA-binding transcriptional regulator", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A636", "UniRef:UniRef90_A0AB32XA88" ], "nt": "ATGTTTAAAGAAATTTATGTAGCAGCAACAACAGCTGGTGGACCAGATCCAGATATGAACCCAGCACTTAAATTAGCTATTGCAAAAGCTAAAAGTAAAAACATGCCTAAAGCAAATATTGAAAGAGCTTTAGCAAAAGCAAAAGGTGATGGTAAAGATGCATCAGCTTATACAGAAGTTTTATTCAATGCAACAATTGCAGGGGGAGCTACATTTTTAGTTGTTGCCTTAACAGATAATATGAATAGAACAACAAGTTCTATGAAAGCACACTTTAATAGACAAAATGCAGCTTTAGGTAAAACAGGTCAAGTACCTTTTGCTTTCGATCATAAAGGTGTTTTAGAAGTTTCAAAAAACTTAGTTGGAGAAGACGATCTAACTATGGTTGCACTTGAAAATGGTGCCGAAGATTTTGAAGTAACTGAAGATTCTTATATAATTACAACAGCACCAGAAAACTTTTCAAAATGTAAAAGCGCTGTTGAAAGTAACTTAAATATTAGTGATTTTATGCAATGTGAAGTTACATATATTCCTAACACAACAGTTGCTTTTGATAAAGAAAAAAGTGAAAAACTTCAAGAATTTATCGCTCGTTTAGAAGATGACGATGATGTTCAAGAAGTATTTCACAATATTGAATTTGAAGAATAA", "aa": "MFKEIYVAATTAGGPDPDMNPALKLAIAKAKSKNMPKANIERALAKAKGDGKDASAYTEVLFNATIAGGATFLVVALTDNMNRTTSSMKAHFNRQNAALGKTGQVPFAFDHKGVLEVSKNLVGEDDLTMVALENGAEDFEVTEDSYIITTAPENFSKCKSAVESNLNISDFMQCEVTYIPNTTVAFDKEKSEKLQEFIARLEDDDDVQEVFHNIEFEE", "aa_hexdigest": "154d2d629acd25bb20dcb6537374bf95", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB32XA88", "query_cov": 1.0, "subject_cov": 0.8685258964143426, "identity": 1.0, "score": 412.0, "evalue": 6.02e-145, "valid": true, "product": "YebC/PmpR family DNA-binding transcriptional regulator", "uniref50_id": "UniRef50_Q4A636" }, "pscc": { "uniref50_id": "UniRef50_Q4A636", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A636" ], "product": "Probable transcriptional regulatory protein MS53_0373" }, "genes": [], "id": "OAIKOHHJFB_1358", "locus": "GCA000209735_01348" }, { "type": "cds", "sequence": "contig_1", "start": 749337, "stop": 749459, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTCTAATGCAAAAATTAGTCAAGAGGTCAAAAATAAAATAGACAAAATGTACAAAAATAGTGAACACAAAAGAAATGTTTCATTACAACCTCAAGAAATTGATACAATATTAAAATAA", "aa": "MDSNAKISQEVKNKIDKMYKNSEHKRNVSLQPQEIDTILK", "aa_hexdigest": "8adb8b6f9a2d635929924fbedc3f5072", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4702.3276000000005, "isoelectric_point": 9.158008003234862 }, "id": "OAIKOHHJFB_1359", "locus": "GCA000209735_01349" }, { "type": "cds", "sequence": "contig_1", "start": 749520, "stop": 750035, "strand": "-", "frame": 3, "gene": null, "product": "NH(3)-dependent NAD(+) synthetase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFL0", "UniRef:UniRef90_C4XFL0" ], "nt": "ATGAAAGAGAGAGTTGCTGATGCTTGTGCTAAAGGAATTGTTTTAGGTATTAGTGGAGGTATAGATTCTGCTGTAGTTGTCGCCTTAGCAAAAAAAGCTTTTGGCAAAAATGTTCTAGGTGTAATAATGCCTGTTGATAGCATGCAAAATGATTTAAAAGATATCAAAGAATTAGAAAAAGCAGTTGATTTAAAATTTATAACTATTGATCTAAAAAATACCTATGATACTATAAAGCAGGCTTTACCGTTAATTAACAGCAATTTAGCTTTAAGTAATATTAAACCAAGATTGCGTATGGCTGTCATATATGCTTTAGCTCAAGAAAACAATTATTTAGTTGCAGGAACAGGTAATAAATGTGAAACTCATATTGGTTATTTTACAAAATATGGTGATGGCGGAAGTGACATTTTGCCTTTAAGTAAACTGTTAAAAAGTGAAGTAAAAATTATTGCAAAGCACTTAAAAGTGCCAGACTCAATTATTAATAAAAAACCTTCTGCCGGTCTTTGA", "aa": "MKERVADACAKGIVLGISGGIDSAVVVALAKKAFGKNVLGVIMPVDSMQNDLKDIKELEKAVDLKFITIDLKNTYDTIKQALPLINSNLALSNIKPRLRMAVIYALAQENNYLVAGTGNKCETHIGYFTKYGDGGSDILPLSKLLKSEVKIIAKHLKVPDSIINKKPSAGL", "aa_hexdigest": "422726d1beec85a006952d65c65dff70", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 749337, "stop": 750149, "observations": { "mutation": [ 749522, 750038 ], "stop": [ 749522, 750038 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFL0", "query_cov": 1.0, "subject_cov": 0.6333333333333333, "identity": 1.0, "score": 327.0, "evalue": 1.61e-111, "gene_start": 1, "gene_end": 171, "reference_start": 39, "reference_end": 209, "reference_sequence": "MKKRGLMKKHIGLRKEAKYEADFDDKKIKAYIDYLISWMKERVADACAKGIVLGISGGIDSAVVVALAKKAFGKNVLGVIMPVDSMQNDLKDIKELEKAVDLKFITIDLKNTYDTIKQALPLINSNLALSNIKPRLRMAVIYALAQENNYLVAGTGNKCETHIGYFTKYGDGGSDILPLSKLLKSEVKIIAKHLKVPDSIINKKPSAGLWEGQNDEDELGFSYKDLDSYLMDSNAKISQEVKNKIDKMYKNSEHKRNVSLQPQEIDTILK" }, "paralog": false, "identity": 0.9888888888888889, "score": 520.0, "evalue": 3.54e-190, "description": "Internal stop codon at 749522, 750038. Nonsense mutation around 749522, 750038.", "psc": { "uniref90_id": "UniRef90_C4XFL0", "product": "NH(3)-dependent NAD(+) synthetase", "uniref50_id": "UniRef50_C4XFL0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFL0", "UniRef:UniRef50_C4XFL0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFL0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFL0" ], "product": "NH(3)-dependent NAD(+) synthetase" } }, "id": "OAIKOHHJFB_1360", "locus": "GCA000209735_01350" }, { "type": "cds", "sequence": "contig_1", "start": 750036, "stop": 750131, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACATATTGGATTAAGAAAAGAAGCTAAATATGAAGCAGATTTTGATGATAAAAAAATTAAAGCTTATATTGACTATTTAATTTCTTGA", "aa": "MKKHIGLRKEAKYEADFDDKKIKAYIDYLIS", "aa_hexdigest": "4cc01b3a973439287611d1f38117fe11", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3731.3213000000005, "isoelectric_point": 9.049894523620605 }, "id": "OAIKOHHJFB_1361", "locus": "GCA000209735_01351" }, { "type": "cds", "sequence": "contig_1", "start": 750740, "stop": 750925, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATTAAATACTTAGAATATTCATTTCAAGTTCTTTTAGGTTTAAATGTAAATGTTACAAATAATATTGTGAAGAGAATTGATAAATCAAATTGGATTGCTGTTGTTATAAAAGCAGTTGATTTGTATTCTTTTTTCATAATACTTTTAATAAATAAATTAAATAACCTTAAGCTGCAATAA", "aa": "MVIKYLEYSFQVLLGLNVNVTNNIVKRIDKSNWIAVVIKAVDLYSFFIILLINKLNNLKLQ", "aa_hexdigest": "811166614d595fe9606d134e30d77b73", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7111.4774, "isoelectric_point": 9.698317527770996 }, "id": "OAIKOHHJFB_1362", "locus": "GCA000209735_01352" }, { "type": "tRNA", "sequence": "contig_1", "start": 751184, "stop": 751257, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(cgt)", "amino_acid": "Thr", "anti_codon": "cgt", "score": 71.4, "nt": "GCCAATTTCGCTTAGTGGTAAAGCAGCTGACTCGTAATTAGCCGCCATGAGTTCGATTCTCATAATTGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 751223, 751225 ], "id": "OAIKOHHJFB_20", "locus": "GCA000209735_01353" }, { "type": "cds", "sequence": "contig_1", "start": 751860, "stop": 751988, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTACTTAATAGAAAAATCAAAATTTACTATTTTAGACCCTCTACCTGTGGCATTAGTTATTATGTCTATTGGTATGTCACTTGGTGGTGTAACAGGTTTTGCACTTAATCCTGCAAGAGACTGA", "aa": "MIYLIEKSKFTILDPLPVALVIMSIGMSLGGVTGFALNPARD", "aa_hexdigest": "48487e3f0f6b8ff31ca0e5460d570a3b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4493.3966, "isoelectric_point": 5.980678367614746 }, "id": "OAIKOHHJFB_1363", "locus": "GCA000209735_01354" }, { "type": "cds", "sequence": "contig_1", "start": 752006, "stop": 752218, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAAAAAAAGCGGAAAAGACCGCTTTTTTAATTTTGATTACATAAATGTTTCAACTAAAGGAATAATGCCAACTAGTAAACCTGCTGCAAAAGGTGTAGCACCAGGAACAATTGAACCATATACTCAATTTGCTGAAGGAGCTTTAACTACTTCTCCTGTTTTTTCATCTTTTATTCCGTCTTTAAAGAAGAATAATACTGTCATTTGA", "aa": "MIKKSGKDRFFNFDYINVSTKGIMPTSKPAAKGVAPGTIEPYTQFAEGALTTSPVFSSFIPSLKKNNTVI", "aa_hexdigest": "0d53abd1446e1a46912379ffe11964e0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7586.693399999997, "isoelectric_point": 9.822999382019042 }, "id": "OAIKOHHJFB_1364", "locus": "GCA000209735_01355" }, { "type": "cds", "sequence": "contig_1", "start": 752219, "stop": 752632, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22421.1" ], "nt": "ATGGAAGAACATAATCAAGCACCGTTTAAACATGTAGCTCAAAGAGTAGTAGCTAGTGAAGTAGTTAGAGATATTTTTGGTCAAGATGAATTAGATTCAGCTATTCAAATTACAGAAGTTTTGTTTAACAAAAATTTTGATACAAGCAAACTAAATTTAAAAGATCTTAAATCAATTGAGCAATATTTACCAGTAGTTGAAATTAAGCAAAATCAAAATATTATTGAAACTTTAATTGAAAAAGGTTTTATTGCTTCAAAACGTGAAGCTAGAGAATTTATGGCAACTAAAGCTTTAAAAATAGATGAAGATATAGTTGAAGAAAACTCAGTTTATAAACCAAAAAACTTTTCTAAAAAGTATGCATTCTTTAGAAAAGGAAAGAAACAAACTATACTATTAAAAACTATTTAG", "aa": "MEEHNQAPFKHVAQRVVASEVVRDIFGQDELDSAIQITEVLFNKNFDTSKLNLKDLKSIEQYLPVVEIKQNQNIIETLIEKGFIASKREAREFMATKALKIDEDIVEENSVYKPKNFSKKYAFFRKGKKQTILLKTI", "aa_hexdigest": "1262a29a1076fc2da7cc82ff67a5a347", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22421.1", "name": "SYY_C-terminal", "length": 81, "aa_cov": 0.5693430656934306, "hmm_cov": 1.0, "evalue": 5.043191372453999e-13, "score": 44.889930725097656, "start": 56, "stop": 133 } ], "seq_stats": { "molecular_weight": 15920.161699999993, "isoelectric_point": 8.70859889984131 }, "id": "OAIKOHHJFB_1365", "locus": "GCA000209735_01356" }, { "type": "cds", "sequence": "contig_1", "start": 752756, "stop": 752869, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTCTAAAGTTGAGGGTGATGATCATAAAGCTGTTGTAGTTACTGCAGACTTATTAACTGATGAAAATGGAAATAAAATTGGTAAATCAACAGGTGGTGGCAAACTTTGA", "aa": "MISKVEGDDHKAVVVTADLLTDENGNKIGKSTGGGKL", "aa_hexdigest": "c08784d71ee5ffecd1a387dce222ba13", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3798.2368000000006, "isoelectric_point": 5.527671623229979 }, "id": "OAIKOHHJFB_1366", "locus": "GCA000209735_01357" }, { "type": "cds", "sequence": "contig_1", "start": 752957, "stop": 753454, "strand": "-", "frame": 1, "gene": null, "product": "Tyrosine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FDW9" ], "nt": "ATGACAATATTAGAAGATTTAAAATTAAGAGGAATATTAAAGCAAATTAGTAATGAAGATAAGTTTAAAAAACTTGATCCTAAGAAAGTTGCTGTATATTGTGGTTTTGATCCAACTGCGCAAAGTTTGCATTTAGGAAACTATGTTTTAATTTCAGTTTTAAAAAGATTTAAGCAATATGGTTTTAAAGTTTATGGACTTATTGGAGGGGCGACAGGAATGATTGGCGACCCTTCATTTAAAGATAGTGAAAGAGTTCTTTTAGATAATAAAACTTTGAAATTAAATAAAAGTAAAATTAGAAAACAATTAGAAGCTCAAGGCTTAGAAGTAATTGACAACATTCAATTTTATGAAAAAATGAATGTGCTTGAATTCTTAAGAGATGCCGGGAAACTTGTTAATGTTTCATATATGATGACTAAGGATTCAGTTCAAAAAAGAATTGAAAGAGGTTTGTCATTTACTGAATTTTCATATCAAGTTTTACAAGGTTGA", "aa": "MTILEDLKLRGILKQISNEDKFKKLDPKKVAVYCGFDPTAQSLHLGNYVLISVLKRFKQYGFKVYGLIGGATGMIGDPSFKDSERVLLDNKTLKLNKSKIRKQLEAQGLEVIDNIQFYEKMNVLEFLRDAGKLVNVSYMMTKDSVQKRIERGLSFTEFSYQVLQG", "aa_hexdigest": "32e2d6135d2d90e58316d6cf03a8d0e8", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A432FDW9", "query_cov": 0.9878787878787879, "subject_cov": 0.9878787878787879, "identity": 0.589, "score": 189.0, "evalue": 1.11e-58, "valid": false, "gene": "tyrS", "product": "tyrosine--tRNA ligase", "ec_ids": [ "6.1.1.1" ], "uniref50_id": "UniRef50_A0A432FDW9", "kegg_orthology_id": "K01866", "go_ids": [ "GO:0000166", "GO:0004831", "GO:0005524", "GO:0006437" ] }, "pscc": { "uniref50_id": "UniRef50_A0A432FDW9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FDW9" ], "product": "Tyrosine--tRNA ligase" }, "genes": [], "id": "OAIKOHHJFB_1367", "locus": "GCA000209735_01358" }, { "type": "cds", "sequence": "contig_1", "start": 753463, "stop": 754158, "strand": "-", "frame": 2, "gene": "tapR", "product": "TyrS-associated PheT N-terminal domain-related protein TapR", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C3CC", "UniRef:UniRef100_C4XFL5", "UniRef:UniRef50_A0A3P8MEK0", "UniRef:UniRef90_C4XFL5" ], "nt": "ATGATGAGTCGACAATTATTGCATATTTCATATTTTATATTATATAATAAAAATATTATGGGTATATTTTTTAATTTAAATTCAAAATTTAAGAACACAAAAATCATATATTTTGATACAACAATAAACAAAAAAACTATGGATATTTATGAAGATATGGTTGTTTTTAGAGATGAAAATTTTAATGTAAATTCGGTTAATTTACTGAATGCAAAAAGTAAAAAAAGAGATGAAAAAATATTTGGTTTTTTAAATGAAAATGAAACTAAAAAACTAGTCAATAAATTGCACAAATATAACAAAAAATATTTACTAAAATCAATTAAATACTTTAGAATTGGTAAAATTGTAGAAAGAAAAAAACACCCAAAAAGCGATAGATTATTTGTATTAAAAATAGATTTTGGAAATGAAGTAAAACAAATAATAACTAACACAACATATACACTTGAAAATAAATATTTTACATTCTGTTTACCAGGATCAATTACAGCTAATGGATTAGAAATTTTAGATGGTAAAATTATGAATGTAGAAAGTGATGGAATGCTTTGCTCAGCAGAATCTTTAGATTTAGAAGCAAAAAGCTATAAAGAATTTGAAGAAGGCTTTTTCAATAATGTTTTAGAAGAAGAAAAACAAAAATATTTAGGCAAAGAAATACAAGAATTATTGCCAGAATTAATAAGCAAATAG", "aa": "MMSRQLLHISYFILYNKNIMGIFFNLNSKFKNTKIIYFDTTINKKTMDIYEDMVVFRDENFNVNSVNLLNAKSKKRDEKIFGFLNENETKKLVNKLHKYNKKYLLKSIKYFRIGKIVERKKHPKSDRLFVLKIDFGNEVKQIITNTTYTLENKYFTFCLPGSITANGLEILDGKIMNVESDGMLCSAESLDLEAKSYKEFEEGFFNNVLEEEKQKYLGKEIQELLPELISK", "aa_hexdigest": "cff29f1e9b65af178a6a0b85e740a348", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C3CC", "uniref100_id": "UniRef100_C4XFL5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C3CC", "UniRef:UniRef100_C4XFL5" ] }, "ips": { "uniref100_id": "UniRef100_C4XFL5", "uniref90_id": "UniRef90_C4XFL5" }, "psc": { "uniref90_id": "UniRef90_C4XFL5", "gene": "tapR", "product": "TyrS-associated PheT N-terminal domain-related protein TapR", "uniref50_id": "UniRef50_A0A3P8MEK0" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MEK0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEK0" ], "product": "Phenylalanyl-tRNA synthetase subunit beta" }, "genes": [ "tapR" ], "id": "OAIKOHHJFB_1368", "locus": "GCA000209735_01359" }, { "type": "cds", "sequence": "contig_1", "start": 754269, "stop": 754505, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAACTTTTACACTAAAAAAGCTAAAGTAAAAACATCGGCTATCAATATAGCTAAAACTACAGAATTATTTGAAAAATTGTCAAAAGAGTATGACAAAATTATTTTCTATCCAATCTCTCATCATTTGTCAAGTACTTATCAAACTTCAAAAACAATGGAAGAAGAGTTTCCAAAATTAAGAACAATTAAATCGGTTCAAATTGTTCAATTAATTGTTTTAGATTTAATTTGA", "aa": "MFNFYTKKAKVKTSAINIAKTTELFEKLSKEYDKIIFYPISHHLSSTYQTSKTMEEEFPKLRTIKSVQIVQLIVLDLI", "aa_hexdigest": "3284f02f1e1b235258535b2266f4a381", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9159.662299999996, "isoelectric_point": 9.398346138000488 }, "id": "OAIKOHHJFB_1369", "locus": "GCA000209735_01360" }, { "type": "cds", "sequence": "contig_1", "start": 754518, "stop": 754898, "strand": "+", "frame": 3, "gene": "degV", "product": "DegV family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFL6", "UniRef:UniRef90_C4XFL6" ], "nt": "ATGGCTAAAGATCCTTCAAAAATGGATGAATATCTTAAATACATTGAAGACGGGGGGTTCCACAAATCAATTACCCTTATACCTAAATACAATGATTATTTAGTTAAAGGTGGAAGACTGCATCCTGCTGCCGCAGCCATAGCAAAATTATTTAAAATTGTTCCTTTAATTGCTGTTGATGGTGGTCAACTTAAAAAAGAAGGCGTCGGTCATGTTTTTTCAAAAACCACTATTAAAAATATAGTATCTAAAGCTAAAAACTTCAAAATCAATGACAAGAAAAATGTACTTCTTACTGCTTATATACACACAGGTGCTTCAGAAGAAGAACAAAAAACTTTTATTGAAGAATTCAAAAAACAATACCACCAAGACCCTTGA", "aa": "MAKDPSKMDEYLKYIEDGGFHKSITLIPKYNDYLVKGGRLHPAAAAIAKLFKIVPLIAVDGGQLKKEGVGHVFSKTTIKNIVSKAKNFKINDKKNVLLTAYIHTGASEEEQKTFIEEFKKQYHQDP", "aa_hexdigest": "6913da68583222c0c0779a18b0f73455", "start_type": "ATG", "rbs_motif": null, "genes": [ "degV" ], "truncated": "both", "pseudogene": { "start": 754218, "stop": 755030, "observations": { "mutation": [ 754503, 754896, 754905, 754995 ], "stop": [ 754503, 754896, 754905, 754995 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFL6", "query_cov": 1.0, "subject_cov": 0.42, "identity": 1.0, "score": 250.0, "evalue": 8.65e-82, "gene_start": 1, "gene_end": 126, "reference_start": 131, "reference_end": 256, "reference_sequence": "MKYAIIVDSSCALTKKQAEALGWFYTPLHINIDEKEFEDGVDINTSNLFNFYTKKAKVKTSAINIAKTTELFEKLSKEYDKIIFYPISHHLSSTYQTSKTMEEEFPKLRTIKSVQIVQLIVLDLIWFEQQMAKDPSKMDEYLKYIEDGGFHKSITLIPKYNDYLVKGGRLHPAAAAIAKLFKIVPLIAVDGGQLKKEGVGHVFSKTTIKNIVSKAKNFKINDKKNVLLTAYIHTGASEEEQKTFIEEFKKQYHQDPWVNWLAPVVAIHTGPEAFSNVAVECPKETKQIFWEKMDKIKDLL" }, "paralog": false, "identity": 0.9851851851851852, "score": 521.0, "evalue": 9.1e-191, "description": "Internal stop codon at 754503, 754896, 754905, 754995. Nonsense mutation around 754503, 754896, 754905, 754995.", "psc": { "uniref90_id": "UniRef90_C4XFL6", "product": "DegV family protein", "uniref50_id": "UniRef50_C4XFL6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFL6", "UniRef:UniRef50_C4XFL6" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFL6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFL6" ], "product": "DegV family protein" } }, "id": "OAIKOHHJFB_1370", "locus": "GCA000209735_01361" }, { "type": "cds", "sequence": "contig_1", "start": 755150, "stop": 755290, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00383.29" ], "nt": "ATGGCTTTAGCTAAAGTATCTGCATTAAGATCTAAAGATCCTTCAACAAAGGTCGGTGCTTGCGTAGTTAATGAAAACAAAAGAATTATTGCTCTAGGTTACAATGGAATGCCAAAAGGCATAGATGACGAATTCCCTTGA", "aa": "MALAKVSALRSKDPSTKVGACVVNENKRIIALGYNGMPKGIDDEFP", "aa_hexdigest": "d7330ea7e3051064131807cc2b734702", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00383.29", "name": "dCMP_cyt_deam_1", "length": 42, "aa_cov": 0.8913043478260869, "hmm_cov": 1.0, "evalue": 1.80121969548692e-08, "score": 30.32900047302246, "start": 1, "stop": 41 } ], "seq_stats": { "molecular_weight": 4905.6738000000005, "isoelectric_point": 8.978270149230958 }, "id": "OAIKOHHJFB_1371", "locus": "GCA000209735_01362" }, { "type": "cds", "sequence": "contig_1", "start": 755348, "stop": 755605, "strand": "+", "frame": 2, "gene": null, "product": "CMP/dCMP-type deaminase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFL7", "UniRef:UniRef90_C4XFL7" ], "nt": "ATGAATGCAATTCTAAATACAAACAATAATCTTCAAGGTTGCACAATCTTCACTTCTTTATATCCATGCTCAAGTTGTGCTAAGACCATAGCTCAATCAGGCATTAGTGAAGTAGTTTATGAAGATGACAAATATCATGACACTGAAGACGCAGAAATAGCAAGGCACATTTTAAATGCAAGCAATATTAAAACAAGAAAAATTGAAATAGATACAAATGTTGAAGTTCAAGTGGGTAAAAACAAATACAAAAATTAA", "aa": "MNAILNTNNNLQGCTIFTSLYPCSSCAKTIAQSGISEVVYEDDKYHDTEDAEIARHILNASNIKTRKIEIDTNVEVQVGKNKYKN", "aa_hexdigest": "804a9da7e38b9b189bcd6b75ea1f94ac", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 755114, "stop": 755605, "observations": { "mutation": [ 755135, 755288 ], "stop": [ 755135, 755288 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFL7", "query_cov": 1.0, "subject_cov": 0.5214723926380368, "identity": 1.0, "score": 167.0, "evalue": 2e-51, "gene_start": 1, "gene_end": 85, "reference_start": 79, "reference_end": 163, "reference_sequence": "MKNKSLYWDGYFMALAKVSALRSKDPSTKVGACVVNENKRIIALGYNGMPKGIDDEFPWDREGESPKETKYAYVVHAEMNAILNTNNNLQGCTIFTSLYPCSSCAKTIAQSGISEVVYEDDKYHDTEDAEIARHILNASNIKTRKIEIDTNVEVQVGKNKYKN" }, "paralog": false, "identity": 0.9877300613496932, "score": 324.0, "evalue": 6.11e-116, "description": "Internal stop codon at 755135, 755288. Nonsense mutation around 755135, 755288.", "psc": { "uniref90_id": "UniRef90_C4XFL7", "product": "CMP/dCMP-type deaminase domain-containing protein", "uniref50_id": "UniRef50_C4XFL7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFL7", "UniRef:UniRef50_C4XFL7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFL7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFL7" ], "product": "CMP/dCMP-type deaminase domain-containing protein" } }, "id": "OAIKOHHJFB_1372", "locus": "GCA000209735_01363" }, { "type": "cds", "sequence": "contig_1", "start": 755829, "stop": 756041, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013354280.1", "SO:0001217", "UniParc:UPI0001E32ED1", "UniRef:UniRef100_C4XFL9", "UniRef:UniRef50_C4XFL9", "UniRef:UniRef90_C4XFL9" ], "nt": "ATGCTATGTCAAACAAGTTATACACAAAGCGTCCAATATTATGAAAAAGCAAATTTTGTTTATATTGTTTTTTCTCTTAAATATGTTTATAGTGATGGCGAAAAAGTATCATTAAAAAATATGACTATTAAAAAATACAAAAAAATCACAAATTATAAAAAAGAACAATTCATTAAAGATAACTTAGAATTGGCAGTTTCGGAGAAATTTTAA", "aa": "MLCQTSYTQSVQYYEKANFVYIVFSLKYVYSDGEKVSLKNMTIKKYKKITNYKKEQFIKDNLELAVSEKF", "aa_hexdigest": "2624e3ad5ee27e47fe5d8d3229bb11f5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32ED1", "ncbi_nrp_id": "WP_013354280.1", "uniref100_id": "UniRef100_C4XFL9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32ED1", "RefSeq:WP_013354280.1", "UniRef:UniRef100_C4XFL9" ] }, "ips": { "uniref100_id": "UniRef100_C4XFL9", "uniref90_id": "UniRef90_C4XFL9" }, "psc": { "uniref90_id": "UniRef90_C4XFL9", "uniref50_id": "UniRef50_C4XFL9" }, "pscc": { "uniref50_id": "UniRef50_C4XFL9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFL9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8399.661499999998, "isoelectric_point": 9.301772499084471 }, "id": "OAIKOHHJFB_1373", "locus": "GCA000209735_01364" }, { "type": "cds", "sequence": "contig_1", "start": 756930, "stop": 759710, "strand": "+", "frame": 3, "gene": null, "product": "Lipoprotein-associated type-17 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFM0", "UniRef:UniRef90_C4XFM0" ], "nt": "ATGAAAAAAATAAAGAAAATAAGTTTGGCTATTGTTCCTGCAATTGCAACAGTAACAACTTTACCGTTGACTACTATTTCATGTCATAAAGATCCTAAGGAAACTCCAAAATCTCTTACTGAAACAGAACTAAATAGTTTATTAGAAAAAATCAAAGTAACTTTATTAGATAAAAATTTAACTGCTGATGAAGTTATAGCTAAAGGCAAAGACATTTTTAAATTTACAGATGTAGATACTAAAGTTAATTTTAAAATTGTATCAATTAAAAAAGGTGCAAATAAAGGTGAAGTTGAATTAACACTTAAAGTTGTTGATAAAAAAGATTCAAATAAATTCTCTAAAGAAAAAACATTTGAAATAAAAACTTTATTAACACCTGATATGGTATTGAATCAAATTAAAGCTGAATATCCAGATCACTTAAATGTGTTTGTTACAGATGCTAATATAAATAAAATTGTTTACTCTAATTTTGACCAAAACACCTTTGAAATAGTTGAATCTAAACTTTTGAGTAAATTTTATTCAACATTAAAATTTGAATTTAAAATTAAAAACAAAATTAGAAATGAAGTTTCTACAAATAAAAGAACTATACAAATTGATGGTTTTAAATTAACAGACAAATATTTAAATTATCAACTCGGAAATATTGAAATTTATTCAGATGGATCTAAAACTTTAAAAGAAACAAAAACTGAACTTTTAAATCCAAATACAACAACATACAAAACAATATTTATTAATAACTTACCATCAACAGTAAAAATTGTTTTTGAATCTGTTGAAGTATATAAAGAAAATGCTGATCAGTTAGAAATTAAATTTCATCTTACAAATATTCACAATGAAAACAATAAATCTAATACTAGAACTGAATACATATATCACAAAAGTGATCATTATGATGCTTTAGTTCAAGAATTTGAAAAAGCTAAATTATCATATGAAGGCGGTACTAACAAAGTAAATGCTTTTGATATTTTTGAAAGCAAAATTAAAATAGAAGGACTTAAAACACCATCAGATTTTTCAGATTTTGTTTTTAAATTCACAAATTCTAGCCCTAGCTCTACTGAATACAAAAGTGGTAAAAGAAAAATTGATTTTAGTTTTGTTTATAAAGGAAACAAATTCTTCAGAAGTGAACAAGAACTTCAAGTAAGACCAACAACGAATGAAATAATTACATCATTAAAAAAACTTAATAAAATTAAACTTGTTGAAACAACTGAAGCTTTAAATAAATTAGAAAGCTTAGTTGATGGTACAACTTTAGATGCATTTAACAATTCTATTTCAATAGATGGGGTTGAATTATTTAGTTACAATTTTACTGCATATCTATTTTCTGAAGATAATGATACTTCAGCTAAAAAAGTATTACTAAATAAAGATAATGAAAACTATTATTTAAGTTTCTACTTAGGTGGAAAAGTATATGGCAAATATGTAAGACACTATGAAGAAAGTAGCACTACTATAGCTAAAATCCAAACACCTGCTGAATTGACAGCAATGGTAAATTCAAAAATTAATGAATTTGATTATAAAAACAAAACAACAACAAAAGCTAAAAATGCCATTCAAGAAAACATTGTTAGCCCAGATTTTGGCTCAGGTTACAGCGTTAAAATTTCAGACTTCACAACTTTAAATGACAAAATTACATTTAATGTTCAATTAAGTAATTCTCAACATCCAGATGTTTTCTCTAATAAAGTTCCAATTACAATTTCAGGTTTCTACCGTAGCGAATTAGCAGAAGAAATCACTAACATGGAAGTTACAATTGCTGATTCATTTAAAGCTAAAGAAGCTAAAGAATTCACAGATGTAAACAACATTACAATCACAGACAAAAGAACAAGTACTGCTTATGATTTTGCAACTAAAGGTGTTTCTCTTGAAACAACTATTGTTGAAGTTAAAAATTTAGAAGGTATTATTAAATTCAACTTAAAATTCACAAAAGAAACTGAAGTTATTAATAAAGAATACATCATTAATGATTTCAAAAAAGAAACTTTAACAGCTGAAAAAATTGTTAAGCTATTAAATATCCAATTAATTAAAGAAAATGTTCAATTAGACAAAAAATACTTCTTGTCTTCATATATTACAAAAAATGAAGTATCTATTACAAACTTAGATGATCTCAAAAAAATAAATTCAACAGTTAATGTTGTTATTAATAAAACAACACCTAATGTTGCTGAAGGTAAATTAGACATTGAATTAAACATTTCGTTTGATTCAAATTCAAATACTAAAACAATTTCAGTCAGTGGCTTTAACAAAAACGTTAAATCTTCAACTGAAAATATGATATTTAAAGACAAAATGAAGTTAATTGTTTACTTGGCAAAAAATAATATTTTGTTCAAACAAGTAGATCAAAACACAATTAATGAAATGAATGTTGCAGGTCCATTTGGCATGACAACATCTATTAAACAAGGCTTAACAAAATATGGTTTTAACCAAATGACAATGGAAAACAACTTCAATTTTGAATATGTATTTAACTTTGTTTCAGCTGATACTGAAACAGTTCAAGATGAAAAATTTGATACTTTAATTAGACATCATATTGTTGGTAATAAATATAATAAAGATCAACCGATGTCTCGCCACATAAAAGTTTTTAGAATAAATGGTAATACACAATTAGCTGCATCATTTAGAATTGTTGCTAGAGATCATGTTAAATTTGATTTAACTAAATTTGAATATAAAACATCAGTTGACGATGATGAATATAAAGTATTTACATGA", "aa": "MKKIKKISLAIVPAIATVTTLPLTTISCHKDPKETPKSLTETELNSLLEKIKVTLLDKNLTADEVIAKGKDIFKFTDVDTKVNFKIVSIKKGANKGEVELTLKVVDKKDSNKFSKEKTFEIKTLLTPDMVLNQIKAEYPDHLNVFVTDANINKIVYSNFDQNTFEIVESKLLSKFYSTLKFEFKIKNKIRNEVSTNKRTIQIDGFKLTDKYLNYQLGNIEIYSDGSKTLKETKTELLNPNTTTYKTIFINNLPSTVKIVFESVEVYKENADQLEIKFHLTNIHNENNKSNTRTEYIYHKSDHYDALVQEFEKAKLSYEGGTNKVNAFDIFESKIKIEGLKTPSDFSDFVFKFTNSSPSSTEYKSGKRKIDFSFVYKGNKFFRSEQELQVRPTTNEIITSLKKLNKIKLVETTEALNKLESLVDGTTLDAFNNSISIDGVELFSYNFTAYLFSEDNDTSAKKVLLNKDNENYYLSFYLGGKVYGKYVRHYEESSTTIAKIQTPAELTAMVNSKINEFDYKNKTTTKAKNAIQENIVSPDFGSGYSVKISDFTTLNDKITFNVQLSNSQHPDVFSNKVPITISGFYRSELAEEITNMEVTIADSFKAKEAKEFTDVNNITITDKRTSTAYDFATKGVSLETTIVEVKNLEGIIKFNLKFTKETEVINKEYIINDFKKETLTAEKIVKLLNIQLIKENVQLDKKYFLSSYITKNEVSITNLDDLKKINSTVNVVINKTTPNVAEGKLDIELNISFDSNSNTKTISVSGFNKNVKSSTENMIFKDKMKLIVYLAKNNILFKQVDQNTINEMNVAGPFGMTTSIKQGLTKYGFNQMTMENNFNFEYVFNFVSADTETVQDEKFDTLIRHHIVGNKYNKDQPMSRHIKVFRINGNTQLAASFRIVARDHVKFDLTKFEYKTSVDDDEYKVFT", "aa_hexdigest": "50863267d315c61d748c9d79f00ce5cb", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_C4XFM0", "query_cov": 1.0896328293736501, "subject_cov": 0.9931102362204725, "identity": 0.915, "score": 1711.0, "evalue": 0.0, "valid": true, "product": "Lipoprotein-associated type-17 domain-containing protein", "uniref50_id": "UniRef50_C4XFM0" }, "pscc": { "uniref50_id": "UniRef50_C4XFM0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFM0" ], "product": "Lipoprotein-associated type-17 domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_1374", "locus": "GCA000209735_01365" }, { "type": "cds", "sequence": "contig_1", "start": 759866, "stop": 760087, "strand": "-", "frame": 1, "gene": null, "product": "S4-like RNA-binding protein", "db_xrefs": [ "KEGG:K14761", "RefSeq:WP_013526579.1", "SO:0001217", "UniParc:UPI0001A8C3D2", "UniRef:UniRef100_C4XFM1", "UniRef:UniRef50_A0A3P8LAR7", "UniRef:UniRef90_C4XFM1" ], "nt": "ATGAATAATGAAATTTATTTAAAAGATGATGTAATTACACTAGGTCAATTATTAAAGAAAATAGGACTTATTGACACAGGCGGATCAGCTAAAGGTTATTTACTTTCTAATAAAATTTTAATAAATAATAGAACACCAAATGGACGTAGTGCAAAAGTTAAAATTGGTGACATTGTGTGGATAAATAATAAAGTTTTTATGATTAAAAAACTTGAAAAATAA", "aa": "MNNEIYLKDDVITLGQLLKKIGLIDTGGSAKGYLLSNKILINNRTPNGRSAKVKIGDIVWINNKVFMIKKLEK", "aa_hexdigest": "9aeb56913a561be2220eaac433b8a17c", "start_type": "ATG", "rbs_motif": "AGxAG", "ups": { "uniparc_id": "UPI0001A8C3D2", "ncbi_nrp_id": "WP_013526579.1", "uniref100_id": "UniRef100_C4XFM1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C3D2", "RefSeq:WP_013526579.1", "UniRef:UniRef100_C4XFM1" ] }, "ips": { "uniref100_id": "UniRef100_C4XFM1", "uniref90_id": "UniRef90_C4XFM1" }, "psc": { "uniref90_id": "UniRef90_C4XFM1", "uniref50_id": "UniRef50_A0A3P8LAR7", "kegg_orthology_id": "K14761" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LAR7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LAR7" ], "product": "S4-like RNA-binding protein" }, "genes": [], "id": "OAIKOHHJFB_1375", "locus": "GCA000209735_01366" }, { "type": "cds", "sequence": "contig_1", "start": 760089, "stop": 760304, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGAAATCGGAACATCTATTGCAAAAACAGAAAATTTTAAATTTGAAGGAAAAGCTTTTGAATTTGATATTAACTATCATTTCTTAAAAGATGCTTTAAGTGTGTTTGAAGGTGACGTTTTAATTCTATTTGATAAAAGTGTACAAAAAATTTTAGTAGTTTCAGATTCAAATAAAGAATCTAAACAACTAGTAACTCCTATGAGAAGATAA", "aa": "MNEIGTSIAKTENFKFEGKAFEFDINYHFLKDALSVFEGDVLILFDKSVQKILVVSDSNKESKQLVTPMRR", "aa_hexdigest": "3b9f9ef2ee1fbc2738233ee09d95103e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8158.273899999998, "isoelectric_point": 5.727858543395997 }, "id": "OAIKOHHJFB_1376", "locus": "GCA000209735_01367" }, { "type": "cds", "sequence": "contig_1", "start": 760377, "stop": 761195, "strand": "-", "frame": 3, "gene": "dnaN", "product": "DNA polymerase III subunit beta", "db_xrefs": [ "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0009360", "KEGG:K02338", "SO:0001217", "UniRef:UniRef50_C4XFM2", "UniRef:UniRef90_C4XFM2" ], "nt": "ATGAAATTACACATTAAAAAACAAATTTTAGATGAAACATTGGAAATTGTTTCAAAGTATACAGATCCTATTAATTCATTGTATGGGTTCAGATGTATATTAATTAAAGTAGATAATGAAAAAGTAACATTTTCAGCATCTAATGGAGTTATAAGTATAGTTAAATCACTAGATGTTGAAGATGTGGATATTAAAGTAGAAGAAACAGGAACATTTTTAATTCAAGCAAATATATTTAAAGCTATTATTAAAAAATTAAGTGAAACAATTACTTTAAGTCAAGAATATTCAACAACATTAGAAATATCACAAGGCAATTCAAATTATTCATTAACTACAAACCCAATCAATTCATTTCCACAAATAGAAGAAAATATTAATTTAAAAAAAATTGAAATCGATACAAATGAATTTAAAAAAGCTATTAGATGCACAGCTTTTGCTGTTAGTCAAGACGCTTCATTAGTATATAAATGTATTAATTTTAAATTTAAAAATAATAAATTAAATCTAACAGCTACAGATGCAGCAAGACTTGCATATTACACAATGGATCTTAAAAGTTCGCAAGTAGATAATGAAGAATTTTCAGTAAACAATAAAGATGTTAAAGATTTAATACCTGCAGATGCATCTAAAAAAGTAACATTATTCTTTAATAGAATAAAAATAGGAATTGAATATAAAAATACAGTTATCACTTCAAGAATAGTTGATTTGCCTTACCCAGATGTTGAAAGTTTATTTGCTTCAATAGATATTGAAAATAAAATATTTATTAAAAAAGAAGAAATAAATGATTTAATTAACAAAGTTTGA", "aa": "MKLHIKKQILDETLEIVSKYTDPINSLYGFRCILIKVDNEKVTFSASNGVISIVKSLDVEDVDIKVEETGTFLIQANIFKAIIKKLSETITLSQEYSTTLEISQGNSNYSLTTNPINSFPQIEENINLKKIEIDTNEFKKAIRCTAFAVSQDASLVYKCINFKFKNNKLNLTATDAARLAYYTMDLKSSQVDNEEFSVNNKDVKDLIPADASKKVTLFFNRIKIGIEYKNTVITSRIVDLPYPDVESLFASIDIENKIFIKKEEINDLINKV", "aa_hexdigest": "a765a82a43c80ac677b227a62e0d1ac2", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "dnaN" ], "truncated": "5-prime", "pseudogene": { "start": 760089, "stop": 761198, "observations": { "mutation": [ 760379 ], "stop": [ 760379 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFM2", "query_cov": 1.0, "subject_cov": 0.7371273712737128, "identity": 1.0, "score": 515.0, "evalue": 7.92e-183, "gene_start": 1, "gene_end": 272, "reference_start": 2, "reference_end": 273, "reference_sequence": "MMKLHIKKQILDETLEIVSKYTDPINSLYGFRCILIKVDNEKVTFSASNGVISIVKSLDVEDVDIKVEETGTFLIQANIFKAIIKKLSETITLSQEYSTTLEISQGNSNYSLTTNPINSFPQIEENINLKKIEIDTNEFKKAIRCTAFAVSQDASLVYKCINFKFKNNKLNLTATDAARLAYYTMDLKSSQVDNEEFSVNNKDVKDLIPADASKKVTLFFNRIKIGIEYKNTVITSRIVDLPYPDVESLFASIDIENKIFIKKEEINDLINKVWLGTSDKQNRLEFTINKNEITVLNRLNEIGTSIAKTENFKFEGKAFEFDINYHFLKDALSVFEGDVLILFDKSVQKILVVSDSNKESKQLVTPMRR" }, "paralog": false, "identity": 0.994579945799458, "score": 707.0, "evalue": 4.9e-261, "description": "Internal stop codon at 760379. Nonsense mutation around 760379.", "psc": { "uniref90_id": "UniRef90_C4XFM2", "gene": "dnaN", "product": "DNA polymerase III subunit beta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_C4XFM2", "kegg_orthology_id": "K02338", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0006260", "GO:0009360" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFM2", "EC:2.7.7.7", "UniRef:UniRef50_C4XFM2", "KEGG:K02338", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFM2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFM2" ], "product": "DNA polymerase III subunit beta" } }, "id": "OAIKOHHJFB_1377", "locus": "GCA000209735_01368" }, { "type": "cds", "sequence": "contig_1", "start": 761326, "stop": 761703, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08299.17" ], "nt": "ATGGAAATGGCCCGGACTAATTCTAAATATACTGTTGCTGTTGTTAATTCAATATTAAAAGACATGCAACAAACAAAAGAAAATATTACACCAGATTCAATAATAGAATATGTAGCAAAATATTACAAAATTCCTAAAAAAGAAATCTTAGGCAAAAGCAGACAAAAAGATGTCGTTCTAGCAAGACACATAGCTATTTATGTTATAAGAACACAACTGGAAGTTCCATTAGAACAAATTGGTCATATATTTGGTAATAGAGATCACTCAACTATTATTAATGCAATTAGAAAAATAGAAACTGAAAGTGATATGCCAGATAAAACAATTAAAAGAACCATATCAATGATTAGTGATGATATCTACAAATTAAAATAG", "aa": "MEMARTNSKYTVAVVNSILKDMQQTKENITPDSIIEYVAKYYKIPKKEILGKSRQKDVVLARHIAIYVIRTQLEVPLEQIGHIFGNRDHSTIINAIRKIETESDMPDKTIKRTISMISDDIYKLK", "aa_hexdigest": "367f31c02b7669217b6976ba446b42c7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08299.17", "name": "Bac_DnaA_C", "length": 69, "aa_cov": 0.552, "hmm_cov": 1.0, "evalue": 8.539969428634634e-28, "score": 92.33539581298828, "start": 31, "stop": 99 } ], "seq_stats": { "molecular_weight": 14478.732099999987, "isoelectric_point": 9.396283149719238 }, "id": "OAIKOHHJFB_1378", "locus": "GCA000209735_01369" }, { "type": "cds", "sequence": "contig_1", "start": 761794, "stop": 762534, "strand": "-", "frame": 2, "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "db_xrefs": [ "KEGG:K02313", "SO:0001217", "UniRef:UniRef50_A0A168RAH0", "UniRef:UniRef90_C4XFM3" ], "nt": "ATGCTAGATGGTAATATTTTAAAAGCTTTAGAAGAAACTTTTGAAAGAGAATGCACATATGAATTTGTTCATATTCCTAAACCTATTGTTAAATTAACAAAAGCCTCAAGCAACAAAACAATTTCTTCTAAAACAATTGAAGAAGAAGTGAAATTAGGCGTTGATTTAAATAAAGAATTAACATTTGACAACTATGTTGAAAGTGATTTTAATAAAGATGCAATCAAATTAGCTAACTATTTAGTTAAAGGTGGAAAAGAATATAATCCTATTTTTATTTATGCTAAAAGTGGACTAGGAAAAACACACTTATTACATGCAATTGGTAACAAATTACAAGAGCAAGGCAAAGTTGTTAAATACATTAATCCAAGCATTTATGCTAGAGATATATCATTCTATTTGCAAGAAAATGATCAAGCAAAATTAAAGAAAATAAAAGATGAATTTGATAATGCTGATTACTTAATATTTGATGATTTTCAAAGTTATGGAGTCGGAAATAAAAAAGCAACAATTCAAACAATTTATAATATTTTAGACTATCGTTTGAACACAAATAAAACAACCATATTTTGTTCGGATAAGCCTATAAATAACTTGAATGCGATGTTTGATGAAAGATTAATTTCTCGTCTTTATATGGGTTTACAACTTGAAATTAAAATTCCACAACAAAGTGATTTATTAAGAGTTTTAGATTACATGCTAGATATTAATGATATGAATCCAGAGGCTTGA", "aa": "MLDGNILKALEETFERECTYEFVHIPKPIVKLTKASSNKTISSKTIEEEVKLGVDLNKELTFDNYVESDFNKDAIKLANYLVKGGKEYNPIFIYAKSGLGKTHLLHAIGNKLQEQGKVVKYINPSIYARDISFYLQENDQAKLKKIKDEFDNADYLIFDDFQSYGVGNKKATIQTIYNILDYRLNTNKTTIFCSDKPINNLNAMFDERLISRLYMGLQLEIKIPQQSDLLRVLDYMLDINDMNPEA", "aa_hexdigest": "437979b0c65b6713510117a09417e7b5", "start_type": "ATG", "rbs_motif": null, "genes": [ "dnaA" ], "truncated": "both", "pseudogene": { "start": 761491, "stop": 762741, "observations": { "mutation": [ 761796, 762570 ], "stop": [ 761796, 762570 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFM3", "query_cov": 1.0, "subject_cov": 0.5222929936305732, "identity": 1.0, "score": 486.0, "evalue": 2.57e-170, "gene_start": 1, "gene_end": 246, "reference_start": 70, "reference_end": 315, "reference_sequence": "MMAENKNIKDSITMKANTLTFLKSLSSDVNDQMLFKNFISNFTIVGIDQNNHVKIQTWLQKPSLEILIKMLDGNILKALEETFERECTYEFVHIPKPIVKLTKASSNKTISSKTIEEEVKLGVDLNKELTFDNYVESDFNKDAIKLANYLVKGGKEYNPIFIYAKSGLGKTHLLHAIGNKLQEQGKVVKYINPSIYARDISFYLQENDQAKLKKIKDEFDNADYLIFDDFQSYGVGNKKATIQTIYNILDYRLNTNKTTIFCSDKPINNLNAMFDERLISRLYMGLQLEIKIPQQSDLLRVLDYMLDINDMNPEAWEKEAKNFVTRNYSTSIRTLIGAITRLKFYKMEMARTNSKYTVAVVNSILKDMQQTKENITPDSIIEYVAKYYKIPKKEILGKSRQKDVVLARHIAIYVIRTQLEVPLEQIGHIFGNRDHSTIINAIRKIETESDMPDKTIKRTISMISDDIYKLK" }, "paralog": false, "identity": 0.9927884615384616, "score": 807.0, "evalue": 2.88e-299, "description": "Internal stop codon at 761796, 762570. Nonsense mutation around 761796, 762570.", "psc": { "uniref90_id": "UniRef90_C4XFM3", "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "uniref50_id": "UniRef50_A0A168RAH0", "kegg_orthology_id": "K02313", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFM3", "UniRef:UniRef50_A0A168RAH0", "KEGG:K02313" ] }, "pscc": { "uniref50_id": "UniRef50_A0A168RAH0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A168RAH0" ], "product": "Chromosomal replication initiator protein DnaA" } }, "id": "OAIKOHHJFB_1379", "locus": "GCA000209735_01370" }, { "type": "cds", "sequence": "contig_1", "start": 762568, "stop": 762738, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGAGAATAAAAATATAAAAGATAGTATCACAATGAAAGCAAATACATTAACATTTTTAAAATCTTTAAGTTCAGATGTTAATGACCAAATGTTGTTCAAAAATTTCATTTCAAACTTCACAATTGTAGGCATAGATCAGAACAATCATGTCAAAATTCAAACATGA", "aa": "MAENKNIKDSITMKANTLTFLKSLSSDVNDQMLFKNFISNFTIVGIDQNNHVKIQT", "aa_hexdigest": "e455e33f97f0962e50210e5d426462f3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6362.225699999999, "isoelectric_point": 8.12064723968506 }, "id": "OAIKOHHJFB_1380", "locus": "GCA000209735_01371" }, { "type": "oriC", "sequence": "contig_1", "start": 762739, "stop": 763028, "strand": "?", "product": "origin of replication", "nt": "AATATAAAAATAGTATTATTTATATTTTAATGTAAGAAATAATTAGTAAGTTTTTTAGTTATTTAAACAAGTTATCCACAAACTTATCAACAATTAAAAGTCTTATTTATAGTATAAAAAGTAGAAAAGTTAACTTATTAACATATGTTTATAACTATGTGGAAAACTGTGTTTAAAAATAAGAATACTCTTTATATATACTTAAAAATTTGATAATATCCTTATTTAAAGACAAAAAAATATTTAATGAAAATGAATATTAAACTTATTTAAATGAGCTCAATAATGTT", "id": "OAIKOHHJFB_1818" }, { "type": "cds", "sequence": "contig_1", "start": 763535, "stop": 764125, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATTAAGTGGGCCTAATAAATATAATAATTACATACTTGAAGGCTACAATATGATTGAAGCAACAAAAGAAGATTTAAAAGTTATGTTGAAGAATCATATTAGAGGTTTTAAAGAAGAAGATCATAATATTTTAAAAGAAAATTTAGGTGAAAATTATTTAGATGTTATTAAAGATACACCTAAAGAAGAAAGAAAAATATTTTACGAATTTATTAAACCCCCTTTGCTTAAAAAAATAGAAGCTTTTATTGAAGAAGTTCTAAACATGGGTAAAGATGAAAGATTTTTTATAACCAATAATATTTACAATTTTTATATTAAATTGAAGCAAAAATTACCTCTTGCAGATGGCGAATCATATTTAGATTTTTTTGATAAAAACAAAGAAAATCATGAAGATCCTTATCTTATTTTGTATATTGAATTAGGATTAAGCTTTGACTCAGTAGACAATTTTGCAAGCTTAATAGGATATTCAAAAGATTGCGAAACTAGATATTATGCAATAGTTTATAAATCTATTTATGATTTTGGAATTCAAGTTAATGATACATATTACAATTTGTCTGATTTACATAAAAATTGA", "aa": "MELSGPNKYNNYILEGYNMIEATKEDLKVMLKNHIRGFKEEDHNILKENLGENYLDVIKDTPKEERKIFYEFIKPPLLKKIEAFIEEVLNMGKDERFFITNNIYNFYIKLKQKLPLADGESYLDFFDKNKENHEDPYLILYIELGLSFDSVDNFASLIGYSKDCETRYYAIVYKSIYDFGIQVNDTYYNLSDLHKN", "aa_hexdigest": "9d758875175af236a0b93ed7cb931a44", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23331.19940000001, "isoelectric_point": 4.96354465484619 }, "id": "OAIKOHHJFB_1381", "locus": "GCA000209735_01372" }, { "type": "cds", "sequence": "contig_1", "start": 764225, "stop": 764818, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12", "PFAM:PF13245.12", "PFAM:PF13604.12", "PFAM:PF00580.27" ], "nt": "ATGCAAAAATTATATTATGCAGAAAAAGAAGTTCCAACTATAGCACTTAGTTTTATTAAAGAACAAGAAGACTTTATTTTTGAAAAATTAATAACTATAAAAAATAAGATAAATAAAACTATTCCGCAAATAGAAGATGATACATTAACTGCTAAACAACTTGATGCTTTAAACATTGCACTAAATGAACCGATAAGCATAATTTCAGGATTCCCTGGCACTGGCAAAACATATTTAATTAGTAATATTTATGAGTCATTAATTGCCCAAAAAGTCTATAAAAAAGGTGAAATAGAAATTTTGACCCCCACTGGTAGAGCTGCAATTAATATTAAAAATAAGAGTAATAATTTGCCTGCAAAAACGATTCACAACTATTTAAAAATTGATAAAGAAGATGAAGACGTTGTTTATAACAATGATTGTGACAAAACGAAAGTTGTAATTATTGACGAGTTTTCAATGGTTAATTTAAAGATTTTTTATCAACTTTTAAGTCATTTATCCAAGCTTGAAAAGCTCATTATTGTTGGAGATAAAGATCAGCTTCCATGTATTGGACCTGGCAATTTAATGCACGACTTATGTAATTGA", "aa": "MQKLYYAEKEVPTIALSFIKEQEDFIFEKLITIKNKINKTIPQIEDDTLTAKQLDALNIALNEPISIISGFPGTGKTYLISNIYESLIAQKVYKKGEIEILTPTGRAAINIKNKSNNLPAKTIHNYLKIDKEDEDVVYNNDCDKTKVVIIDEFSMVNLKIFYQLLSHLSKLEKLIIVGDKDQLPCIGPGNLMHDLCN", "aa_hexdigest": "a5d67d5ae5ef378a748e56494718cd55", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 55, "aa_cov": 0.25888324873096447, "hmm_cov": 1.0, "evalue": 1.341396456165003e-11, "score": 40.78759002685547, "start": 49, "stop": 99 }, { "id": "PF13245.12", "name": "AAA_19", "length": 138, "aa_cov": 0.6903553299492385, "hmm_cov": 0.9710144927536232, "evalue": 4.560332218209119e-34, "score": 113.59687042236328, "start": 53, "stop": 188 }, { "id": "PF13604.12", "name": "AAA_30", "length": 151, "aa_cov": 0.751269035532995, "hmm_cov": 0.9271523178807947, "evalue": 7.48671869159256e-26, "score": 87.11857604980469, "start": 49, "stop": 196 }, { "id": "PF00580.27", "name": "UvrD-helicase", "length": 72, "aa_cov": 0.36548223350253806, "hmm_cov": 0.9722222222222222, "evalue": 7.27142211975833e-14, "score": 48.144962310791016, "start": 49, "stop": 120 } ], "seq_stats": { "molecular_weight": 22330.68700000001, "isoelectric_point": 5.688355445861816 }, "id": "OAIKOHHJFB_1382", "locus": "GCA000209735_01373" }, { "type": "cds", "sequence": "contig_1", "start": 764948, "stop": 765091, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAAATAAACTCAGTTGCATTATTAAACGAATTATCATACTTATATCAAACTTATAATCCAGAATACAATTCTGAAAAAATGGTAACCTTGATTCCACTTCATGATGGTCAAGTTGGAGCCAAAACAGTTAATAAATGA", "aa": "MKEINSVALLNELSYLYQTYNPEYNSEKMVTLIPLHDGQVGAKTVNK", "aa_hexdigest": "9eaa5eb767de2ec2b7712779c9ddf157", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5358.0606000000025, "isoelectric_point": 5.5737112045288075 }, "id": "OAIKOHHJFB_1383", "locus": "GCA000209735_01374" }, { "type": "cds", "sequence": "contig_1", "start": 765452, "stop": 765607, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAATAAAAAACTTTTATATACAGCAGTTTCAAGAGCTAAAGAAAAATTATTTATTTTAGGCGACTTAGCTTTATACGCTAAAAAAACAAAAACTGAAGATTATAGTAAAATAAAAACATATTTATTTGATTTAATTAAGGAACAAAATTAA", "aa": "MLNKKLLYTAVSRAKEKLFILGDLALYAKKTKTEDYSKIKTYLFDLIKEQN", "aa_hexdigest": "6a4f5b27229a6941212a3248b91eafb7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6000.0543, "isoelectric_point": 9.60180835723877 }, "id": "OAIKOHHJFB_1384", "locus": "GCA000209735_01375" }, { "type": "cds", "sequence": "contig_1", "start": 765633, "stop": 765779, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFM5", "UniRef:UniRef90_C4XFM5" ], "nt": "ATGTTTATAACTAATACAAATGAACGAATGGTTTTAAACAAATTAAAAAATCACAACGTGAATGGTGTTTTAGCTGTTTTTGAATCTTTTGATATCAACAAAATGGTTTATAACAAAATAAAAGGTTATATTTCACATCAAATTTGA", "aa": "MFITNTNERMVLNKLKNHNVNGVLAVFESFDINKMVYNKIKGYISHQI", "aa_hexdigest": "e9c9e18933e5ad8ccf7977c8570a74e9", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XFM5", "query_cov": 1.0, "subject_cov": 0.8275862068965517, "identity": 1.0, "score": 98.2, "evalue": 3.61e-26, "valid": true, "uniref50_id": "UniRef50_C4XFM5" }, "pscc": { "uniref50_id": "UniRef50_C4XFM5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFM5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5627.521000000001, "isoelectric_point": 9.60174388885498 }, "id": "OAIKOHHJFB_1385", "locus": "GCA000209735_01376" }, { "type": "cds", "sequence": "contig_1", "start": 765805, "stop": 766185, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013355067.1", "SO:0001217", "UniParc:UPI0001E330B2", "UniRef:UniRef100_C4XFM6", "UniRef:UniRef50_C4XFM6", "UniRef:UniRef90_C4XFM6" ], "nt": "TTGTGTTCAATTAATTACAATTTTGATGATAAAAAAAAGATCATTAATTATTATTCTGAAGATTCACAAGTATTAAAACTTAAAGGCTCATTCCGTGCGAACAAAAATATTGAAGAATTCTATGAAAGTATTTTAGAAAACAAACCATTTCACTTTGATGGTGAAAATGTAATTGCATTTAATCAAAATGATATGGCTAGAAAATACGAAGCTCTCAAATATTATTATAATTATATTGACTCGAAGTTTATCGATTCATCAAAAAATATTAAAGAATGTTTCCCAAATTATAAAGATGAACTTTTTGTTATTTATGCACTTCTAAAAGAATTTTATGGGTGCAATTATCCAGAAGGAAAACCAACTGAGTTGAAAAAGTAG", "aa": "MCSINYNFDDKKKIINYYSEDSQVLKLKGSFRANKNIEEFYESILENKPFHFDGENVIAFNQNDMARKYEALKYYYNYIDSKFIDSSKNIKECFPNYKDELFVIYALLKEFYGCNYPEGKPTELKK", "aa_hexdigest": "de79ea82f2bb0fc4db5e2b84f9c6e752", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E330B2", "ncbi_nrp_id": "WP_013355067.1", "uniref100_id": "UniRef100_C4XFM6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330B2", "RefSeq:WP_013355067.1", "UniRef:UniRef100_C4XFM6" ] }, "ips": { "uniref100_id": "UniRef100_C4XFM6", "uniref90_id": "UniRef90_C4XFM6" }, "psc": { "uniref90_id": "UniRef90_C4XFM6", "uniref50_id": "UniRef50_C4XFM6" }, "pscc": { "uniref50_id": "UniRef50_C4XFM6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFM6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15124.932399999989, "isoelectric_point": 5.89718189239502 }, "id": "OAIKOHHJFB_1386", "locus": "GCA000209735_01377" }, { "type": "cds", "sequence": "contig_1", "start": 766467, "stop": 766640, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330B1", "UniRef:UniRef100_A0AB32XD64", "UniRef:UniRef50_A0AB32XD64", "UniRef:UniRef90_A0AB32XD64", "PFAM:PF13538.12" ], "nt": "TTGAATTATTATAAAGTTCAAGGTTCTGAATCTGATGTAGTTATCATTTACTTTGACAAAAACATAGCCAAATATATTACTAAAAATCACTTATATACTGCTCTTTCAAGAGCTAAAAAGAAAATCATTATTATGAATGATGTAGATGAATTTAAAGAACAGTACCTAAACTAA", "aa": "MNYYKVQGSESDVVIIYFDKNIAKYITKNHLYTALSRAKKKIIIMNDVDEFKEQYLN", "aa_hexdigest": "8a3a66c0030187053a56bd7e3f5b5554", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E330B1", "uniref100_id": "UniRef100_A0AB32XD64", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330B1", "UniRef:UniRef100_A0AB32XD64" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XD64", "uniref90_id": "UniRef90_A0AB32XD64" }, "psc": { "uniref90_id": "UniRef90_A0AB32XD64", "uniref50_id": "UniRef50_A0AB32XD64" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XD64", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XD64" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13538.12", "name": "UvrD_C_2", "length": 45, "aa_cov": 0.7368421052631579, "hmm_cov": 1.0, "evalue": 9.325244963306285e-11, "score": 37.5797119140625, "start": 3, "stop": 44 } ], "seq_stats": { "molecular_weight": 6808.7852, "isoelectric_point": 8.89220485687256 }, "id": "OAIKOHHJFB_1387", "locus": "GCA000209735_01378" }, { "type": "cds", "sequence": "contig_1", "start": 766707, "stop": 767267, "strand": "+", "frame": 3, "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "db_xrefs": [ "EC:3.1.1.29", "GO:0004045", "RefSeq:WP_013527204.1", "SO:0001217", "UniParc:UPI0001A8C3D9", "UniRef:UniRef100_C4XFM8", "UniRef:UniRef50_Q6KHA3", "UniRef:UniRef90_C4XFM8" ], "nt": "ATGAAATTAATCGTAGGTTTAGGTAATCCAGGAAACCAATATCGTTTTACAAAACACAATATTGGTTTTTTAACTGTCGACAAAATTTGTGCGAACTTTAATTTAACTCTTACAAAAGAAAAATTTAATGGCTTTTATGCAAAAACTGAAGATTTTATTTTAGCTAAGCCAACTACTTATATGAATAATTCTGGTGAATTTGTTCAAGCGATAGCTTCATTTTATAAAATAGATCCTTCAGATATTTTGATTATTCATGATGAAAAGGATTATGAAATTGGTCAAGCAGCAATTAAAGTAGGCGGTTCAAGTGCTGGACATAATGGTGTTCAAAGCATAATTAATGCTCTTAGAAGCAATGAATTTAAGAGGCTTAGAATAGGAATCGGAAGAGATCCTAAAATGGAATTAAAAGATTATGTTTTAAGTAATTTTACAAGTGATCAAATGTTAGTAGTCGAACAAGTTCTAAATGTTGCTGCACAAGCTGCAATATCTTTTGTTTACAATGATATTAAGATAGTAATGAATAACTTTAATGTTTTTCGTAAAGGTAAATAA", "aa": "MKLIVGLGNPGNQYRFTKHNIGFLTVDKICANFNLTLTKEKFNGFYAKTEDFILAKPTTYMNNSGEFVQAIASFYKIDPSDILIIHDEKDYEIGQAAIKVGGSSAGHNGVQSIINALRSNEFKRLRIGIGRDPKMELKDYVLSNFTSDQMLVVEQVLNVAAQAAISFVYNDIKIVMNNFNVFRKGK", "aa_hexdigest": "9457996ec59eb25c1fe2297bb449555a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C3D9", "ncbi_nrp_id": "WP_013527204.1", "uniref100_id": "UniRef100_C4XFM8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C3D9", "RefSeq:WP_013527204.1", "UniRef:UniRef100_C4XFM8" ] }, "ips": { "uniref100_id": "UniRef100_C4XFM8", "uniref90_id": "UniRef90_C4XFM8" }, "psc": { "uniref90_id": "UniRef90_C4XFM8", "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "ec_ids": [ "3.1.1.29" ], "uniref50_id": "UniRef50_Q6KHA3", "go_ids": [ "GO:0004045" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KHA3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KHA3" ], "product": "Peptidyl-tRNA hydrolase" }, "genes": [ "pth" ], "id": "OAIKOHHJFB_1388", "locus": "GCA000209735_01379" }, { "type": "cds", "sequence": "contig_1", "start": 767269, "stop": 767634, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATATTTTAGGAATTAGTGGCGGCCCAGATTCAATGCTACTTTTACATGAATATGCTTATAAAAAAGAGCCCATTTTTGTAGTGTGCATTAATTATAATCAAAGGATGGATTCACATAAAGATGTGGAAATTGTAGAAAATTATTGCAAACAAAATTTTATAGATTATCAAATATACAATTTTGACCAAAAAGACTATAAAACAGGCAATTTTGAAGAATGGGCCAGAGACAAAAGAATGGAAATCTTTATTCAAAATTATCATGAAAAGAATGCTTCTAAAATTTTGTTAGCACACCACAAAGATGATTTTTTAGAGTCATTTTTTATGCAAAAAGTAGCGAAAAGAAAACCTTCATTTTGA", "aa": "MYILGISGGPDSMLLLHEYAYKKEPIFVVCINYNQRMDSHKDVEIVENYCKQNFIDYQIYNFDQKDYKTGNFEEWARDKRMEIFIQNYHEKNASKILLAHHKDDFLESFFMQKVAKRKPSF", "aa_hexdigest": "515da2feb5bd3437c7b54be267890d98", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14633.543999999987, "isoelectric_point": 6.506780052185058 }, "id": "OAIKOHHJFB_1389", "locus": "GCA000209735_01380" }, { "type": "cds", "sequence": "contig_1", "start": 768218, "stop": 768526, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAAAAACCAAGCATACTGTCAATTATTTTAATTACAGTATTGTCATTCTTAGCTTTATCAATCGTTTCATATTATTTGTTTAAAAGATTTGGACCTAAGCCTCAAATTGTTGACTTAAATCAATTTATGAATGATTTAAAAGCTGCTTCAGCTAGTCAATCAGATGATGTTTATTTTGCAAGTATAGAGTTTGACTCGTATTATGGAACTGCAAAATATGTATATGCTGGTTTAGACTTAAAAGGTAACCGCATATTAGTTGAAAGAATTGCTCAAATTGGAAGATCTAATGCAGCATCATGA", "aa": "MQKKPSILSIILITVLSFLALSIVSYYLFKRFGPKPQIVDLNQFMNDLKAASASQSDDVYFASIEFDSYYGTAKYVYAGLDLKGNRILVERIAQIGRSNAAS", "aa_hexdigest": "e4fc9cd3f6dad3d53c507396bf652e80", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11379.032299999993, "isoelectric_point": 9.221702766418456 }, "id": "OAIKOHHJFB_1390", "locus": "GCA000209735_01381" }, { "type": "cds", "sequence": "contig_1", "start": 768761, "stop": 770311, "strand": "+", "frame": 2, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AS56" ], "nt": "ATGAAAAAAAATATGAGTATGATGGGTGCAATTGGAGATGTAAAGAATCCTGCAAGAAAAGTCAAATCAGATAAAACATTTAAAGATGTTGCAGGTAACAAAGAAGCAATTGAAGAAATTGAAGAAATTGTTGATTATCTTAAAAATCCTAAAAAATATGCACTTGCTGGTGCTAGAATGCCACATGGTATTTTACTTGGAGGCCCTACAGGGACAGGTAAAACATTGTTAGCTAAAGCTACAGCAGGTGAAGCAAATGTACCTTTCTACTTTATTTCAGCTTCAAACTTTGTTGAAATGTATGTTGGTTTAGGTGCTAAAAGAGTTAGAACTGTTTTTGCAGAAGCTCGTAAAAATGCTCCAGCTATTATCTTTATTGATGAATTAGATGCTATTGGTCGTACCCGTGGAGCTGGTTTAGGCGGAGGTCATGATGAACGTGAACAAACACTTAACGAACTTCTTGTTGAAATGGATGGTATGACTCAAAATAATGGAATATTAATTTTTGCAGCAACTAACCGTACAGACGTTTTAGACCCTGCTTTAACTCGTCCAGGTCGTTTTGACAGAACAATTACAGTTGGATTACCTGATGTTAAAGAACGTGAAGAAATTTTAGAATTGCATGCTAAAGGTAAAAGATTATCACCTGAAATTAAACTTGATCAAGTTGCAAAAAGAACTCCAGGATTTTCTGGCGCTCAACTTGAAAATGTTATTAATGAAGCCTCATTATTAGCAGTTAGAGCTAACTCAGACGTTATTAGACGTGAAGATATTGATGAAGGTATCGATAGAGTTATGGCTGGACCTGCTAAGAAAAATAGAGTTATTACTAAAGAAGAACTTACAATGGTTGCTTACCACGAAGCCGGTCATGCTGTTGTTGGATTAAAAGTTAAAGGTGGAAACAAAGTTCAAAAAATTACCATTATTCCTCGTGGAAATGCCGGTGGTTACAACTTAATGACACCCGAAGAAGAAAAATATAATGCAACAAAACAAGAATTGATTGCAATGATTACTGGATTTATGGGTGGTCGTGCATCTGAAGAATTAATTTATGGAAAAGACAATGTTTCTACAGGCGCTAGTGATGATATTTCTAAAGCAACCAAAATTGCTCGTAGAATGGTAACTGAATTTGGTATGTCACAACTTGGCCCTATTCAATATGAAAAAGATGAAAGCTCTCCATTCTTAGGCCGTGATTATCTAAAAAGTGCTTCATTTAGTTCTCAAGTAGCCCACGAAATTGATCTTGAAGTTAGAAGAATAATGACAGAAGCAGAAAAGAATGCCGCTAAAATCATTAAAGAAAACAGACAACTTCTTGAATTAATCAAGACAGCTTTACTTGAAAATGAAACAATTGTTGCTGAAGAAATTGAATACATTGAAAAGAATATGAAACTTCCTCCAAAAAGAGAAGCTGACAAAATCAAAACCAAAGTTTATACTTTAGATGAGCTTATCAATGAACACAAAGATGACAAAAAATTAGAAGATTCAAAAGCTGAAACAAAAAAAGATAATTCTAAAAAATAA", "aa": "MKKNMSMMGAIGDVKNPARKVKSDKTFKDVAGNKEAIEEIEEIVDYLKNPKKYALAGARMPHGILLGGPTGTGKTLLAKATAGEANVPFYFISASNFVEMYVGLGAKRVRTVFAEARKNAPAIIFIDELDAIGRTRGAGLGGGHDEREQTLNELLVEMDGMTQNNGILIFAATNRTDVLDPALTRPGRFDRTITVGLPDVKEREEILELHAKGKRLSPEIKLDQVAKRTPGFSGAQLENVINEASLLAVRANSDVIRREDIDEGIDRVMAGPAKKNRVITKEELTMVAYHEAGHAVVGLKVKGGNKVQKITIIPRGNAGGYNLMTPEEEKYNATKQELIAMITGFMGGRASEELIYGKDNVSTGASDDISKATKIARRMVTEFGMSQLGPIQYEKDESSPFLGRDYLKSASFSSQVAHEIDLEVRRIMTEAEKNAAKIIKENRQLLELIKTALLENETIVAEEIEYIEKNMKLPPKREADKIKTKVYTLDELINEHKDDKKLEDSKAETKKDNSKK", "aa_hexdigest": "30c967a6eebb0786c7bc6dbd82f278d3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A449AS56", "query_cov": 0.9166666666666666, "subject_cov": 0.9594320486815415, "identity": 0.7440000000000001, "score": 694.0, "evalue": 2.57e-247, "valid": false, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "uniref50_id": "UniRef50_A0A449AS56", "go_ids": [ "GO:0004222", "GO:0006508", "GO:0016020", "GO:0016887", "GO:0051301" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449AS56", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AS56" ], "product": "ATP-dependent zinc metalloprotease FtsH" }, "genes": [ "ftsH" ], "id": "OAIKOHHJFB_1391", "locus": "GCA000209735_01382" }, { "type": "cds", "sequence": "contig_1", "start": 770645, "stop": 771337, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF4EEE1", "UniRef:UniRef90_UPI000FF4EEE1" ], "nt": "ATGGTTAATGGTTTTAATGATTATAAACATGTATCAAAATTGCAAGATATCAATGTTGACTTAAATTTACCAACACAAGGTAGTGCATATAATCAAGGTTACTACATTAATGGTGAAAAATCAGATGATTTCAAAGGGACAAATAAAGACTATACACCAATTGAATATCAAATTGATTATCAAAGTGTAAAAGCTTCTTCATTGCCCGATTCAACTAAAAAAGAATATGAAGACTTTACAAGAAATGTTTTATTTTTTGGCAATGATAAAAGAGAATATTTGCCATTTAATAAAATAGGTAATATTGACCCTTCGGTAAATAGTTATTACTTTAAAAAAGATGACTTATTAAATCTTTACTCAATTTATAAAGCTGAACAAAACATTGAAAATAATAAAAACCCAGACATTGTTTTTGATTTAAAAGACATAAATAATTTAACTTTTGAACTAAAACAAAAAAACCGTTTCTTGAATATTTATGAAAAGAAAGGCGATACTTATGAACAAGTTTTTCTTGAAAAGAAACTTGAAAGAACTAACATTGTTCATTTCGATAAAACCTATAAAATAGGTAATTTAATGAATTTAACTATCAACAATAATGAAATTTATGCAACTCAATTAAGTTCATTATTTATTAATACTGACACAAAGGAAATAACAGCAGACTATTCAAAAGGTAAAAGTTGA", "aa": "MVNGFNDYKHVSKLQDINVDLNLPTQGSAYNQGYYINGEKSDDFKGTNKDYTPIEYQIDYQSVKASSLPDSTKKEYEDFTRNVLFFGNDKREYLPFNKIGNIDPSVNSYYFKKDDLLNLYSIYKAEQNIENNKNPDIVFDLKDINNLTFELKQKNRFLNIYEKKGDTYEQVFLEKKLERTNIVHFDKTYKIGNLMNLTINNNEIYATQLSSLFINTDTKEITADYSKGKS", "aa_hexdigest": "597a5b5683f5512ce0c003bbf20d6f55", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 770519, "stop": 771637, "observations": { "mutation": [ 770522, 771335, 771497, 771536 ], "stop": [ 770522, 771335, 771497, 771536 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000FF4EEE1", "query_cov": 1.0, "subject_cov": 0.49356223175965663, "identity": 0.991, "score": 456.0, "evalue": 8.05e-159, "gene_start": 1, "gene_end": 230, "reference_start": 43, "reference_end": 272, "reference_sequence": "MWRLFKEVFKSLSKNKVIVAGLSILVFLTSAIFTLLSNVSSSMVNGFNDYKHVSKLQDINVDLNLPTQGSAYNQGYYINGEKSDDFKGTNKDYTPIEYQIDYQSVKASSLPDSTKKEYEDFTRNVLFFGNDKREYLPFNKIGNIDPSVNNYYFKKDDLLNLYSIYKAEQNIENNKNPDIVFDLKDINNLTFELKQKNRFLNIYEKKGDTYEQVFLEKKLERTNTVHFDKTYKIGNLMNLTINNNEIYATQLSSLFINTDTKEITADYSKGKSWINQEKGIEIEPKQLAALFNFEPASFDGSTPNGLLFKQKDKTTNLDKFIKYNSSWAEINENLNLQSDWNLEDVFATNPKAIKAQKKLTLNKNNKFTLPIEWIAKEENIVRFLRWNYYTSFVDKDKSKDKWLGAFKTFMEDLIESSKTNPENKKMLDKLTTFSYWRKSKKNIVTPYKSDKTLDLSKKLQKNLLFH" }, "paralog": false, "identity": 0.967741935483871, "score": 713.0, "evalue": 1.82e-262, "description": "Internal stop codon at 770522, 771335, 771497, 771536. Nonsense mutation around 770522, 771335, 771497, 771536.", "psc": { "uniref90_id": "UniRef90_UPI000FF4EEE1", "uniref50_id": "UniRef50_UPI000FF4EEE1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000FF4EEE1", "UniRef:UniRef50_UPI000FF4EEE1" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000FF4EEE1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF4EEE1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 26928.64820000004, "isoelectric_point": 5.372160148620606 }, "id": "OAIKOHHJFB_1392", "locus": "GCA000209735_01383" }, { "type": "cds", "sequence": "contig_1", "start": 772892, "stop": 773395, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTTTTTAAGTCCAAAATCTCAATTAATTTATGATGTTTTAAATCATGGAAAAATTGATTATTTAGCTAATGCAATTGAAAAATATTTATTAAATTTTGATTTAGTTAAACAACAATTTATTACACCTGAGCAAGTTTATCAATTAACAAAAGTTATGAAAAAAGTGTTAAATGATAACAACTTTGCTTCAGTGTTTGCTACTGGCAAAATGAACAAGAAAATATTACCTAAATTAATGTTTGACTTTGTTTATGAGTTAAGTCATTATGAAGGTGGTGATGTTCTCAAATCACTATTCTTTAGCATTCTAGAACAAGCAAAAGCTAAAATAGCTTCTAAATCAACTTTAGTTGAACAAAAAGCTTATTTAGCAGCTGAAGTAAATAACTTATACAAATTACTACAAGATTTAGCTTCTATTGATTTAAACCAATACTTAAGTGCAGAATCATTAGTTAATGTTTCAAATGATCCAAAAATATTTATTAATGCAATTTGA", "aa": "MLFLSPKSQLIYDVLNHGKIDYLANAIEKYLLNFDLVKQQFITPEQVYQLTKVMKKVLNDNNFASVFATGKMNKKILPKLMFDFVYELSHYEGGDVLKSLFFSILEQAKAKIASKSTLVEQKAYLAAEVNNLYKLLQDLASIDLNQYLSAESLVNVSNDPKIFINAI", "aa_hexdigest": "ec3adc94fcbe2a614e966504f7d60dc4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19022.938800000007, "isoelectric_point": 7.803462791442873 }, "id": "OAIKOHHJFB_1393", "locus": "GCA000209735_01384" }, { "type": "cds", "sequence": "contig_1", "start": 773660, "stop": 774442, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCCTTCATTAATTAGTGGTTTAAAACCTATTATTAAAAAAATGAATAGCGATCCAACAAATCCTTATTCTAATGTAAAACAAGGCTTAATTAATATTCTATCAAATATTGATTTTAATATTCTGGCTCGTGAATTAAATCAAACAATAACAACACATTATATTGATTACAAAGTAGTTAAATTTAATCCAGACTTAAACAAAGATGAAGAAAAAGTCTTTTCAGTTGTTTTAAATGCAATAAGTCCAAAAGATGGAATTATATCATTCTTAAGATCTATGTTTGGTATGCCTGGATCAAATAGAGCATTTAAAGAAAACTTAATCAAAATGTTCAATTTGTCTGACAAATATAAAGAAATAGAACTACCTAATAGTAGTGTAAAAATTTATGTTCCAGATAATGACGACACAAAAGTAGGTTTCTTTGATTTCTTAAAAATTTTTGCTTCAGGTTTATCAAATACAGAAGCTAATGAATTCAATAACTACAAAATTGAACATGACTTCTTAGCACTTAAATCATCAATTGAAAGTCTTAAAGAGGAAGGCACTCAAATTAATTTAGATTACTTAAAAGAACCTCAAAAAAGATTACTTAAACAATATGGTTTATTTGTCACAAAAACCTATAAAACAGTCATATTAGAAAAAATTAATAAAATATTAAGGTTCATAAAACAAACAAAAGGTGGAACTTCATATATAAGTAATGATGCTAATAAAACAGGTAGTGATTTATTAAATGATTTAAAAAATTTTGAAAACAACGGGACTTGA", "aa": "MNPSLISGLKPIIKKMNSDPTNPYSNVKQGLINILSNIDFNILARELNQTITTHYIDYKVVKFNPDLNKDEEKVFSVVLNAISPKDGIISFLRSMFGMPGSNRAFKENLIKMFNLSDKYKEIELPNSSVKIYVPDNDDTKVGFFDFLKIFASGLSNTEANEFNNYKIEHDFLALKSSIESLKEEGTQINLDYLKEPQKRLLKQYGLFVTKTYKTVILEKINKILRFIKQTKGGTSYISNDANKTGSDLLNDLKNFENNGT", "aa_hexdigest": "d5257342e868eee1ed4996e18030c368", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29618.561300000027, "isoelectric_point": 8.91064281463623 }, "id": "OAIKOHHJFB_1394", "locus": "GCA000209735_01385" }, { "type": "cds", "sequence": "contig_1", "start": 774545, "stop": 774814, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATGAATAAATATGCAAATCATGAAGAAGCAAAAAGATTTGTTAAAGATTTAATTGCATTAGCATTGGAACCTGAAATATTTGACATTAACAAAAAAATGTCAACAAATGACAATATACCTTTTGCTGGTGTAACTAACTATGGATTATTAGAATTACTTGAAAATCCCGAAAAATCAAACTTATTTGCTTATGATGTTAATGGCAAATTCTTAAATGACAAAGTTGAACAATTTGCTGCTAAAAATGAAATATATAGAAAATGA", "aa": "MFMNKYANHEEAKRFVKDLIALALEPEIFDINKKMSTNDNIPFAGVTNYGLLELLENPEKSNLFAYDVNGKFLNDKVEQFAAKNEIYRK", "aa_hexdigest": "df9ebb9feec64cabbf409eb29d0c0f55", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10334.68269999999, "isoelectric_point": 5.366248893737793 }, "id": "OAIKOHHJFB_1395", "locus": "GCA000209735_01386" }, { "type": "cds", "sequence": "contig_1", "start": 774932, "stop": 776074, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACAATTATTTATTAAGCAAAGATTTTTATGACATTAAAGACCAAGCTCAAATCATTATGTCTTCATTAAATCTTAATGTACCATTACAAATTTTTGGTTTAAGTGAAGCCTTAGTCAATCCTATTTTAAGATTTACTTTCCCTGAAATTACATTATCTTATTTAGCAAGTCAAAAAACAAATGCTAGTTTAGTTAAAGCTAACTTACAATACTTATTGCTCAATAAAATAGATGATTTCGAAAAAATTGTTAGAAAAGGCGAAGACAAATTTTATGCCTTAACTAACATGTTAGGAACTGCATTTAGCAAAAAAGATACCTCACTAATTCCTCTTGACTTAGATGACGAACAAACATTAGTAATGGATGGAGCTACATTTGATAAATTAAAAGATACAAGCTCTTCATTATTTGGAATTGATTTTATTAAATTTGTTAATGAAGCAATGAACACGATAGTTGAACCTAAAGAAATGAAGGACATTGTGTTCTCAAATTCAAATAGTTACTTAGCTAAAGTTAACTATGCATACTTAGCAAAAAATAATAAAGCAATTTATACAGGTAAAATTCCAAGTGATCCTATTGAACTATTATCATTATTAGATAAATTAGATGAAAAATTCATTTTAAATGTTAATGGAACCAAATTTATTATTATTGGTCAAGAAACTACAGTCGACTATATGTACCCAGTTATTGATGAAAATAACTTACAAGTTAATACACAAAACCAATCCTTAGTTTATGTTAACAAATATGGTTTTGACAGAATTCACTCAGGTTACATTGGAAATGTTATTAAGAAAACTCTTTTAGTTAAAAACTCAGCAGCAACTGGTATGAATAATAATGAACTTAAAAAATTCATTACTAAAATAATAGATAACTCAATTGCAGACAGTAACAAACTTCAAAGAGTTTTCTTAGCAAATGAAATTGATCCAATTAACCCTGAAAGGTCTCTTAGAATAACAACTGTTCAAGGAATTATTAAAGCTGTTTCATCAGCTACTATTGCCTTAGTTTCAGTATTCGTTACACTTGTGGCTATTTCAACTATCTTTATTATTAAGAGATATATTTCAAATAAAAACAAAGTTATTGGTATTTTAGTGGCCCAAGGTTATCGTCCATGA", "aa": "MNNYLLSKDFYDIKDQAQIIMSSLNLNVPLQIFGLSEALVNPILRFTFPEITLSYLASQKTNASLVKANLQYLLLNKIDDFEKIVRKGEDKFYALTNMLGTAFSKKDTSLIPLDLDDEQTLVMDGATFDKLKDTSSSLFGIDFIKFVNEAMNTIVEPKEMKDIVFSNSNSYLAKVNYAYLAKNNKAIYTGKIPSDPIELLSLLDKLDEKFILNVNGTKFIIIGQETTVDYMYPVIDENNLQVNTQNQSLVYVNKYGFDRIHSGYIGNVIKKTLLVKNSAATGMNNNELKKFITKIIDNSIADSNKLQRVFLANEIDPINPERSLRITTVQGIIKAVSSATIALVSVFVTLVAISTIFIIKRYISNKNKVIGILVAQGYRP", "aa_hexdigest": "1c27aa503d074da4304bab2b68231356", "start_type": "TTG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 42683.90679999998, "isoelectric_point": 8.625112342834473 }, "id": "OAIKOHHJFB_1396", "locus": "GCA000209735_01387" }, { "type": "cds", "sequence": "contig_1", "start": 776096, "stop": 776443, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGCTTTAGTTACATCAGTTATTGGTGGAATCTTAGGTTATGTAATTGGTTATAAACTACAACTGATGGTTATGAACATCTTTAGTAGTTATTGGACACTGCCAAAACAAACGATTTCATTTAACTTCTTTACATTATTCTTCACTGTCTTCTTACCATTTATTGGAATGAGCTTATTGATCTTTATAGTTTCATTATTTGCTTTAAGACATAAAGCTATAGACTTAATTAGTGGAGTTAATGAAATACCTCAATCAAAATTATTCAAAACTTATCAAAAAGCAACTAAGAAATTTAATGTTAAAAAACGTTTCTCATTTACATTGGCATTTACAAGTTTTTGA", "aa": "MFALVTSVIGGILGYVIGYKLQLMVMNIFSSYWTLPKQTISFNFFTLFFTVFLPFIGMSLLIFIVSLFALRHKAIDLISGVNEIPQSKLFKTYQKATKKFNVKKRFSFTLAFTSF", "aa_hexdigest": "0ee163dd4830ab23af7e54c735a63c13", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13219.757899999975, "isoelectric_point": 10.250618171691894 }, "id": "OAIKOHHJFB_1397", "locus": "GCA000209735_01388" }, { "type": "cds", "sequence": "contig_1", "start": 776471, "stop": 776827, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAACAGGTATCGCAACCTTATTTGGTGTAGCCAATACAAACGTCTTTAATAGAACTATTAATGATACTTATAAAAATAGAAGTTATACATTTAAAGTTGATTTAGAAAGTCCAACAGTTGAAGGTGGAGCATTCAAACCATTTAATCCTGATAATTTATATAACAACATTTATACTCCTCTTGGTGATATAAATGAAGGTAACAGAGAAATAGGAGATTACTTTAAACCTGGTGCTTCTAGTGTGCTTAATAAAGGAACTAAAAATGGTAGTCCTCAAGATTTAGACTCACATATCTTAAGCCAATTCTCAGTTAATGTTACTGTTGCAGCTGGTGTAGCCGCTGACCCTTGA", "aa": "MLTGIATLFGVANTNVFNRTINDTYKNRSYTFKVDLESPTVEGGAFKPFNPDNLYNNIYTPLGDINEGNREIGDYFKPGASSVLNKGTKNGSPQDLDSHILSQFSVNVTVAAGVAADP", "aa_hexdigest": "f5b39e5df9c5293b34a1be4edd37b25b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12720.910799999987, "isoelectric_point": 4.898975563049316 }, "id": "OAIKOHHJFB_1398", "locus": "GCA000209735_01389" }, { "type": "cds", "sequence": "contig_1", "start": 776846, "stop": 777061, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAGATACTCAAAAAGCTAAAATTGATAAGATAAGAGACCGTGTTGGTTATGAGTTAGAAAGAACTCAAGAAGACAAAGATAAAGAATTCTTTGTAGATCCTAATACTTATGCTTTAAGCTTAGTAAATAAGGCTAATAAGGAAAAATTAAGCTTCTTTAAATACTATCATTCACCATATGAAAAACAAGGTAGTTTCAAATATGCTTACTGA", "aa": "MPDTQKAKIDKIRDRVGYELERTQEDKDKEFFVDPNTYALSLVNKANKEKLSFFKYYHSPYEKQGSFKYAY", "aa_hexdigest": "31a20d61db17d34b6d3ea38f3af27a1e", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8552.5075, "isoelectric_point": 8.777128791809083 }, "id": "OAIKOHHJFB_1399", "locus": "GCA000209735_01390" }, { "type": "cds", "sequence": "contig_1", "start": 777086, "stop": 777241, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAAATATAACAACCGATAAATATCGTGATGAATTTAGAACACTCTTAATCAATGGTTATACTAAAATTGCTCAAAAAATTAAAGCCGAAAAAGAAAATCCAAGTTTAATTAAACCTAAAGAAAAGAAACCAACCAATTTATCTGATTACTGA", "aa": "MVNITTDKYRDEFRTLLINGYTKIAQKIKAEKENPSLIKPKEKKPTNLSDY", "aa_hexdigest": "fa64605c08a83b0df022737fa660076d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5985.860800000001, "isoelectric_point": 9.522189903259278 }, "id": "OAIKOHHJFB_1400", "locus": "GCA000209735_01391" }, { "type": "cds", "sequence": "contig_1", "start": 777485, "stop": 777844, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTGTAAATGAAGTTACTTCGAAGAAATATAACATTAATATAGGCGATTTAGTCCAATTTGAAGTTCTTAACCATACAAAACGTTATGCAAACAAAGTGTTGTCAAAAATAGATAAATCATTAAGAAGTAATGTTGACTTAAAAAATCCAAAAGTAACATTTAAAGTTGTTGGTATTAACCCTACATACATTAATAATGAATTAATAACAACAGTTGATGCAGCTAATAAATTAGTTGGCTTAGACAAACTTCCAAATGTTGTTGGATTTAAAGCCTTCAATGGTGTTATGACAAATAGTCCAGCGCCTAGCCAAGTTCTTGATTCAACTTCGCTTTATTCATTAAGTGGTTATTGA", "aa": "MVVNEVTSKKYNINIGDLVQFEVLNHTKRYANKVLSKIDKSLRSNVDLKNPKVTFKVVGINPTYINNELITTVDAANKLVGLDKLPNVVGFKAFNGVMTNSPAPSQVLDSTSLYSLSGY", "aa_hexdigest": "f0482b84fd1f95324d9ceab74f8b55fa", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13089.918299999987, "isoelectric_point": 9.487763786315917 }, "id": "OAIKOHHJFB_1401", "locus": "GCA000209735_01392" }, { "type": "cds", "sequence": "contig_1", "start": 777899, "stop": 778270, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGAACAAATTTATAATCCAGATACTGGAATATTAAAAACCAGATTGAATCTAACAAAAGATCAAATTATGAATTTATTAGATTCAACTGAATTAACATTTAATAAAGCTAAATATGATCAAATTAAAAATGCGCCTAAAAACGCAATAGAAAATTATGCTAAATTGTATGATTCTAAACATTATATTGCTTTAGGTACAAGTATTGATTCAAAAGATATAGAAGCTGGATTTACAACCCAAATTGGTTCTACTATAGGAACAATTAGTATTGCAATTATTGCAATTAGTTTCATAATTTCACTTGTTATTCTTATTATTATGTCAACAATTATGATTAGTGAAAATGAGAAAAACATTGCTATTTGA", "aa": "MFEQIYNPDTGILKTRLNLTKDQIMNLLDSTELTFNKAKYDQIKNAPKNAIENYAKLYDSKHYIALGTSIDSKDIEAGFTTQIGSTIGTISIAIIAISFIISLVILIIMSTIMISENEKNIAI", "aa_hexdigest": "7f65b0cc7a1b904e96c718b69efd4c03", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13690.737799999984, "isoelectric_point": 5.278318977355956 }, "id": "OAIKOHHJFB_1402", "locus": "GCA000209735_01393" }, { "type": "cds", "sequence": "contig_1", "start": 778560, "stop": 779645, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "RefSeq:WP_041594241.1", "SO:0001217", "UniParc:UPI0002EFB0FC", "UniRef:UniRef100_UPI0002EFB0FC", "UniRef:UniRef50_C4XFN2", "UniRef:UniRef90_C4XFN2" ], "nt": "GTGTCTGAAGAATTAGAAAACAAAGAAAAAAAAGTTAAAAAAGATCAAACTTCTACTAAAAAATCAACAGCCAAAAAAATGACCAAAAAGTCTGCAAAAAAGGCTGAAAGAGAAAAAAATAAAAAAGAAGAAACTGGTCTTTTACAAGCTATTATTGAAGAAGAAAAACTTCTTGAAGAACAAGTCGCTGAATTAGAAATTGACAAAAATATCAAAATTACTAAAGGCAATGTTTTTGAGCTTTTAGACAGAGATAGTTCTAAAAAGAATATTATTGTTCCTAAAAAAGTTCGAAAATTATTGACTAAGTTAGCTAAGAAAAAAATAAAAAAAGATGGCAATGAAAACCTAAAAAATGAAGAAGGTAATGTTATTGAAGTTAAGAACGTTAGCAAATATTATGTTAATGGTAATATTGTTACAAGAGTTTTAAAAGATGTTTCAATTGAAATTAAAAAAGGTGAGCTAATGCTTATTTATGGTGTTAGTGGTGGTGGTAAAAGTACACTTCTAAACCTTATTTCAGGACTTGATAGACCTTCAAAAGGACAAGTTATTGCTTGCGATAATAATCTTCCTTATATGTCTGATAGTCAATTAACTCTATTTAGAAGAAAACATGTTAGTTTCATTTTCCAAAACTATAACTTGTTAGCAAACTTAAATGCTTTTGACAATGTTCAAACGGGAGCTTACTTACAAAAAAATCCTGCTAAAAAAGTTGATGTTGTTGAGTTATTTAAGAAATTTGAAATGGATGAAGAAATATCAAAATTCCCTGCTCAAATGTCTGGTGGTCAACAACAACGTGTTTCAATTATGAGAGCCTTAGCTAAAAATGCAGATATCATTTTTGCTGATGAACCTACTGGTGCTCTTGATGAAGCAACAACAAAAATAGTTTTAAGATTTCTTTACAATATTAATAAAGAAACAGGCGCTACAGTTGTTATGGTTAGTCATAACCCAGTTATGGCGCAAATGGCTGATAGAATTATTCATGTTGTTAAAGGAAGAATTGCAAAAGTTGAAGTTAATCAAAAACCAATTCACCCTGATGATATTGAACTTTTTAAAGAAAAATAA", "aa": "MSEELENKEKKVKKDQTSTKKSTAKKMTKKSAKKAEREKNKKEETGLLQAIIEEEKLLEEQVAELEIDKNIKITKGNVFELLDRDSSKKNIIVPKKVRKLLTKLAKKKIKKDGNENLKNEEGNVIEVKNVSKYYVNGNIVTRVLKDVSIEIKKGELMLIYGVSGGGKSTLLNLISGLDRPSKGQVIACDNNLPYMSDSQLTLFRRKHVSFIFQNYNLLANLNAFDNVQTGAYLQKNPAKKVDVVELFKKFEMDEEISKFPAQMSGGQQQRVSIMRALAKNADIIFADEPTGALDEATTKIVLRFLYNINKETGATVVMVSHNPVMAQMADRIIHVVKGRIAKVEVNQKPIHPDDIELFKEK", "aa_hexdigest": "68d6dd84791bdf1c12664b08f1191abc", "start_type": "GTG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0002EFB0FC", "ncbi_nrp_id": "WP_041594241.1", "uniref100_id": "UniRef100_UPI0002EFB0FC", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002EFB0FC", "RefSeq:WP_041594241.1", "UniRef:UniRef100_UPI0002EFB0FC" ] }, "ips": { "uniref100_id": "UniRef100_UPI0002EFB0FC", "uniref90_id": "UniRef90_C4XFN2" }, "psc": { "uniref90_id": "UniRef90_C4XFN2", "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_C4XFN2" }, "pscc": { "uniref50_id": "UniRef50_C4XFN2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFN2" ], "product": "ABC transporter domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_1403", "locus": "GCA000209735_01394" }, { "type": "cds", "sequence": "contig_1", "start": 779830, "stop": 779952, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTACCTTTAGCAATTTTTGGAAAAAATCATTATGAAGCTAGAATAGTTTCATATATTTTTACTGCTATTGGGATAGGAATACTATGCTATGAATATTTCAAAGCTAATCATTTAAAATGA", "aa": "MVPLAIFGKNHYEARIVSYIFTAIGIGILCYEYFKANHLK", "aa_hexdigest": "887ccf7e2e2e6a56cadadfc2068deb6d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4605.4251, "isoelectric_point": 8.932368659973147 }, "id": "OAIKOHHJFB_1404", "locus": "GCA000209735_01395" }, { "type": "cds", "sequence": "contig_1", "start": 779974, "stop": 780087, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTATACTACCTACTGTAGTTTTACCGGTTAATAACTTTACTGAGTTTATTTTTGTTTCTCATCCTTTAGAATTTCATCCTAGTTTTATTCAAGAAATAGCAAAAGAATGA", "aa": "MLILPTVVLPVNNFTEFIFVSHPLEFHPSFIQEIAKE", "aa_hexdigest": "ad9ae4d054a84fca641b04350f50ab53", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4298.0075, "isoelectric_point": 5.015893363952636 }, "id": "OAIKOHHJFB_1405", "locus": "GCA000209735_01396" }, { "type": "cds", "sequence": "contig_1", "start": 780109, "stop": 780261, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTCTGTTGCTTTTATTATTATTGAATATATAACAAGAAATAATATGAGTTTTGAAGATCGTTTGGTTAGAGGAATACTAATGTTTATTTCTCTATACGTAATAGTCAACACTTCAAAATTGATTCAAATAACCATTATACTTGATTGA", "aa": "MISVAFIIIEYITRNNMSFEDRLVRGILMFISLYVIVNTSKLIQITIILD", "aa_hexdigest": "985fba476d0b234caad08870561bcc04", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5834.993599999998, "isoelectric_point": 6.045929527282715 }, "id": "OAIKOHHJFB_1406", "locus": "GCA000209735_01397" }, { "type": "cds", "sequence": "contig_1", "start": 780502, "stop": 780672, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTTTCTTTTCGATATTAGGTGATTTATACTTTTCATATGTTAAAAGAATAAATGCGATAAAAGATTATTCAAAAATTTTAATAAGTCATGGTGGTATTTTAGATCGTTTTGATAGTGTTGCTTTAGGAGGATTAACTTTTCTTTTGTTGTTTGTTATATTTATATAA", "aa": "MPFFSILGDLYFSYVKRINAIKDYSKILISHGGILDRFDSVALGGLTFLLLFVIFI", "aa_hexdigest": "16c6ce4dbdaf0ed75b8b19a0bce215ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6359.517399999997, "isoelectric_point": 8.1192289352417 }, "id": "OAIKOHHJFB_1407", "locus": "GCA000209735_01398" }, { "type": "cds", "sequence": "contig_1", "start": 780722, "stop": 781144, "strand": "+", "frame": 2, "gene": null, "product": "Nucleotidyl transferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XDA2", "UniRef:UniRef90_A0AB32XDA2" ], "nt": "ATGGCAAAAGTTAGAAATGCTGTTATTTTTGCAGCAGGTAAGGGGACTAGAATGACCCCATTAACTCATTATGTTCCTAAACCATTAATAACTGTAAATGATGAATCAATGATTGAAAGAAACATAAAGCATTTACAAGAAATAGGCATTAATGACATCACTGTGGTTGTTGGTTATATGAAAGACCAATTTAATTTAATTAAAGAAAAATATAATGTCAAATTAATTGAAAATGATTTATATGATGAAGCAAACAACATTTATTCTTATGTTGTTTCCAAAAATGCATTTGGAGACACTTTATATATTGAAGGTGATTTGTATATTGAAAAAAATATTTTTCCAGAAATAATTAAAATAATTGAAAACTCTGAAGATTCAATTTGTTTTACTGAAAAATGTACAAAACACAAATCGGAATGA", "aa": "MAKVRNAVIFAAGKGTRMTPLTHYVPKPLITVNDESMIERNIKHLQEIGINDITVVVGYMKDQFNLIKEKYNVKLIENDLYDEANNIYSYVVSKNAFGDTLYIEGDLYIEKNIFPEIIKIIENSEDSICFTEKCTKHKSE", "aa_hexdigest": "723778bd553b296ea32e140b7f45037c", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 780722, "stop": 781447, "observations": { "mutation": [ 781142, 781217, 781280, 781328 ], "stop": [ 781142, 781217, 781280, 781328 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XDA2", "query_cov": 1.0, "subject_cov": 0.56, "identity": 1.0, "score": 281.0, "evalue": 2.09e-94, "gene_start": 1, "gene_end": 140, "reference_start": 1, "reference_end": 140, "reference_sequence": "MAKVRNAVIFAAGKGTRMTPLTHYVPKPLITVNDESMIERNIKHLQEIGINDITVVVGYMKDQFNLIKEKYNVKLIENDLYDEANNIYSYVVSKNAFGDTLYIEGDLYIEKNIFPEIIKIIENSEDSICFTEKCTKHKSEWVFDTNNKGYVVKHHNEKDAYNQNIWIGILYLNKKLANEAKEKVDAWFNQQGNKQQYFETFLWTLENKMRLEAISSNLIKELDNFQDLINIDHHYANHASTLLFTPGPIK" }, "paralog": false, "identity": 0.983402489626556, "score": 473.0, "evalue": 2.75e-172, "description": "Internal stop codon at 781142, 781217, 781280, 781328. Nonsense mutation around 781142, 781217, 781280, 781328.", "psc": { "uniref90_id": "UniRef90_A0AB32XDA2", "product": "Nucleotidyl transferase", "uniref50_id": "UniRef50_A0AB32XDA2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XDA2", "UniRef:UniRef50_A0AB32XDA2" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB32XDA2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XDA2" ], "product": "Nucleotidyl transferase" } }, "id": "OAIKOHHJFB_1408", "locus": "GCA000209735_01399" }, { "type": "cds", "sequence": "contig_1", "start": 781346, "stop": 781981, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00266.25" ], "nt": "ATGAGATTGGAAGCAATTTCTTCAAATTTGATTAAAGAACTTGACAATTTTCAAGACTTAATTAATATTGATCATCATTATGCAAACCACGCTTCTACTTTATTATTTACACCAGGTCCTATTAATAATTATCCAGAAGTTTCTGAGATTTTATCTGAGTCAGTGTTGCATCATAGAAGTGAGTTGTTCAAACACTACATGGAAGAATCAACTAATTTAATTAAAGAATTCTTTAGAACTAAAGAAGCACTTCCATTATTTATTACATGTTCAGCAACTGGGGCTATGGAAGCAGTTGTTGTTAACCTTATTAGTCCTGAAAACAAAGTTTTATTGGTTGAAGCAGGTGACTTTGGTAAAAGATTCCAAATAATTTTAGAAAGATTAATTGGAGCTAATAATCTTGATGTTATTCATTATGAAGATGGTGAAGCATTCAAAGTAAAAGATATAGAAAATAAACTTAAAGAAAATAAATATGATGCATTCTTCTGTACTCATCATGAAACATCAACAGGTGTTTTAAATGATATTGAAGCTATTTCAAAAGTAGTTAAAAAATATGCTAAAGATACATTATTTATTGTGGATACAGTTAGTTCATTTATTCATGAAAATGTTGAGTTTGATAATTGA", "aa": "MRLEAISSNLIKELDNFQDLINIDHHYANHASTLLFTPGPINNYPEVSEILSESVLHHRSELFKHYMEESTNLIKEFFRTKEALPLFITCSATGAMEAVVVNLISPENKVLLVEAGDFGKRFQIILERLIGANNLDVIHYEDGEAFKVKDIENKLKENKYDAFFCTHHETSTGVLNDIEAISKVVKKYAKDTLFIVDTVSSFIHENVEFDN", "aa_hexdigest": "d7889436650376bf54f1bb44a5349ef5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00266.25", "name": "Aminotran_5", "length": 160, "aa_cov": 0.7393364928909952, "hmm_cov": 0.975, "evalue": 1.3914147916298341e-15, "score": 53.21034240722656, "start": 54, "stop": 209 } ], "seq_stats": { "molecular_weight": 24076.008600000026, "isoelectric_point": 5.088192558288574 }, "id": "OAIKOHHJFB_1409", "locus": "GCA000209735_01400" }, { "type": "cds", "sequence": "contig_1", "start": 782027, "stop": 782536, "strand": "+", "frame": 2, "gene": null, "product": "Aminotransferase class V", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEM3", "UniRef:UniRef90_A0AB32XD79" ], "nt": "ATGCCTGGTTTAAGTTGTGTTGTTTTATCTAAAAGAGCTCAACAAGTAGTTAAAGCAAATAAAAACTTTAAATTCTATTTTGATTTATCAGCTTATATTAATTTTTATGAAAGAGAAAAATCAACTCCTTATACTCAAGCTTCAGGTATTTTATTAGCTTTGAATGCTGCAATGAAAGTTATCAAAAACCAAACATTTAAGAATATAAGAAATAATAAACTCAAAATATATGACTACATTAAAAAAGAATTAATTGCTATTGGATTTAAGGATGTAGTTCCTAAAAAGAATATAACTATTGGACTTTTAGTTTTAAATGTTCCAGAAGGTGTTGATGCAATTAAGTTAAGAAATAAAATTGAATATAATCACAATATTTATTTTGAATTAGGTCGTGGAAATAGAAGAAGTAAACAAGTCAGAATTGGAATTCCTAATACTATAGACATGGAAAAAGCTAAAAAACTTGTTAAAGTAATTAAGGAAACTTTAAAGATTAAAATTGATTAA", "aa": "MPGLSCVVLSKRAQQVVKANKNFKFYFDLSAYINFYEREKSTPYTQASGILLALNAAMKVIKNQTFKNIRNNKLKIYDYIKKELIAIGFKDVVPKKNITIGLLVLNVPEGVDAIKLRNKIEYNHNIYFELGRGNRRSKQVRIGIPNTIDMEKAKKLVKVIKETLKIKID", "aa_hexdigest": "0f8ad428041c63473f68e5d870fe95f1", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 781727, "stop": 782536, "observations": { "mutation": [ 781979 ], "stop": [ 781979 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XD79", "query_cov": 1.0, "subject_cov": 0.49415204678362573, "identity": 0.9940000000000001, "score": 322.0, "evalue": 7.86e-109, "gene_start": 1, "gene_end": 169, "reference_start": 174, "reference_end": 342, "reference_sequence": "MLHHRSELFKHYMEESTNLIKEFFRTKEALPLFITCSATGAMEAVVVNLISPENKVLLVEAGDFGKRFQIILERLIGANNLDVIHYEDGEAFKVKDIENKLKENKYDAFFCTHHETSTGVLNDIEAISKVVKKYAKDTLFIVDTVSSFIHENVEFDNWGLDVAIATSGKAFCVMPGLSCVVLSKRAQQVVKANKNFKFYFDLSAYINFYEREKSTPYTPASGILLALNAAMKVIKNQTFKNIRNNKLKIYDYIKKELIAIGFKDVVPKKNITIGLLVLNVPEGVDAIKLRNKIEYNHNIYFELGRGNRRSKQVRIGIPNTIDMEKAKKLVKVIKETLKIKID" }, "paralog": false, "identity": 0.9925650557620818, "score": 525.0, "evalue": 3.37e-191, "description": "Internal stop codon at 781979. Nonsense mutation around 781979.", "psc": { "uniref90_id": "UniRef90_A0AB32XD79", "product": "Aminotransferase class V", "uniref50_id": "UniRef50_A0A3P8MEM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XD79", "UniRef:UniRef50_A0A3P8MEM3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MEM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEM3" ], "product": "Soluble hydrogenase 42 kDa subunit" } }, "id": "OAIKOHHJFB_1410", "locus": "GCA000209735_01401" }, { "type": "cds", "sequence": "contig_1", "start": 782624, "stop": 783319, "strand": "-", "frame": 1, "gene": "rplA", "product": "50S ribosomal protein L1", "db_xrefs": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "KEGG:K02863", "RefSeq:WP_013355058.1", "SO:0001217", "UniParc:UPI0001E33088", "UniRef:UniRef100_C4XFN5", "UniRef:UniRef50_Q4A5E1", "UniRef:UniRef90_C4XFN5" ], "nt": "ATGTCTCTTAAAGGTGGAAAGAAAATAAGAAATGCTCGTGAATCATTTGACAAAACAGTTGCATATGATTTAAAAGAAGCATTAGAAATTGTTAAAAGAACATCATTTACAAAATTTGTTCCTACAGTAGAATTAGTATTCAAATTAAACCTTGACGTTAGAAAAGCTGACCAACAATTGCGTGGATCAGTATTGTTGCCTAACGGTACAGGTAAAAGCGTTCGTGTATTAGTTGTTTCAAACAATCCAGCAATTCAAAAATCATCAAAAGCTGCTGGTGCAGACATCGTTGTTGATGGTCCAGCACTTGAACAAAAAATTAAAGAAGACGAATTTGACTTTGATGTTATGGTTGCTGACCCTTCAATGATGCCTTTATTAGGTAAATATGGTAAAAAACTTGGACCTAAAGGTTTAATGCCAAACCCTAAAACAGGTACAGTTACACCTACTCCTGATAAAACAGTTGCTGAACTTAAAAAAGGTAAAGCTAACTACCGTACAGATAAAGCAGGTATTGTACATACAATCATTGGTAAATTAAATATGACAACAGACCAATTAGCAGAAAATGCTCAAACAATCATTAATTTAATTAAAAAACTTAAACCAGCTACAGTTAAAGGTGCTTACATGCAAAACTTAGTTGTAACATCAACAATGGGTCCTGGTGTTAAAGTTAAAATTGAAAAATAA", "aa": "MSLKGGKKIRNARESFDKTVAYDLKEALEIVKRTSFTKFVPTVELVFKLNLDVRKADQQLRGSVLLPNGTGKSVRVLVVSNNPAIQKSSKAAGADIVVDGPALEQKIKEDEFDFDVMVADPSMMPLLGKYGKKLGPKGLMPNPKTGTVTPTPDKTVAELKKGKANYRTDKAGIVHTIIGKLNMTTDQLAENAQTIINLIKKLKPATVKGAYMQNLVVTSTMGPGVKVKIEK", "aa_hexdigest": "54a24d6dbe97b10a979f8eab9baf07d5", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E33088", "ncbi_nrp_id": "WP_013355058.1", "uniref100_id": "UniRef100_C4XFN5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33088", "RefSeq:WP_013355058.1", "UniRef:UniRef100_C4XFN5" ] }, "ips": { "uniref100_id": "UniRef100_C4XFN5", "uniref90_id": "UniRef90_C4XFN5" }, "psc": { "uniref90_id": "UniRef90_C4XFN5", "gene": "rplA", "product": "50S ribosomal protein L1", "uniref50_id": "UniRef50_Q4A5E1", "kegg_orthology_id": "K02863", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5E1" ], "product": "Large ribosomal subunit protein uL1" }, "genes": [ "rplA" ], "id": "OAIKOHHJFB_1411", "locus": "GCA000209735_01402" }, { "type": "cds", "sequence": "contig_1", "start": 783319, "stop": 783921, "strand": "-", "frame": 2, "gene": "rplK", "product": "50S ribosomal protein L11", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02867", "RefSeq:WP_013527199.1", "SO:0001217", "UniParc:UPI0001F5998C", "UniRef:UniRef100_C4XFN6", "UniRef:UniRef50_A0A3P8KMF6", "UniRef:UniRef90_A0A3P8KMF6" ], "nt": "ATGGCAAAATCAAAAGCTGATATTGTCCGTGTTCGTAAGCTACAATTCATCGCTGGCCAAGCCAAACCTGGTCCAGCTTTAGCTGGTGTTGGAATCAATATGCCTGATTTCACACGTGCTTTTAATGACGCAACACGTGATAGAGGTAATGAACCAGTTCCTGTTGAAATTACCGTTTACAAAGATAAATCATTTGACTACAAGTTATTTACAGCTCCAGCTTCGTACAAATTAAAGCAAGCTGCTAAATTAAACAAAGGTAGTTCAAACTCAAAAACAACAATTGCTGCTACTATTACAAAAGAACAATTGAGAGAAATTGCTGAATACAAATTACCAGACTTAAACAAAAATGATGTTGAAGCTGCTATGAGAACTATTGCTGGAACAGCAAGACAAATGGGTATTATTATCGAAGGATTTGATAACGTTGCTGCTAAAAAAGCTGCTGCTGCTAAAGCTGCAAAAGACAACGCAGCTGCTGCAGCTAAAGAAGCCGCTTTAGATGAAGCTCAAAACGAACTAAAAGAATCTAAAGGTAAAACCATTGATGTTAAAACAATTTCAGACGAAAAAGCTGCTGCTAAAGAAGGAGAAGAATAA", "aa": "MAKSKADIVRVRKLQFIAGQAKPGPALAGVGINMPDFTRAFNDATRDRGNEPVPVEITVYKDKSFDYKLFTAPASYKLKQAAKLNKGSSNSKTTIAATITKEQLREIAEYKLPDLNKNDVEAAMRTIAGTARQMGIIIEGFDNVAAKKAAAAKAAKDNAAAAAKEAALDEAQNELKESKGKTIDVKTISDEKAAAKEGEE", "aa_hexdigest": "933d8bed8a9340a5efe79a2164d8641c", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001F5998C", "ncbi_nrp_id": "WP_013527199.1", "uniref100_id": "UniRef100_C4XFN6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5998C", "RefSeq:WP_013527199.1", "UniRef:UniRef100_C4XFN6" ] }, "ips": { "uniref100_id": "UniRef100_C4XFN6", "uniref90_id": "UniRef90_A0A3P8KMF6" }, "psc": { "uniref90_id": "UniRef90_A0A3P8KMF6", "gene": "rplK", "product": "50S ribosomal protein L11", "uniref50_id": "UniRef50_A0A3P8KMF6", "kegg_orthology_id": "K02867", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KMF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KMF6" ], "product": "Large ribosomal subunit protein uL11" }, "genes": [ "rplK" ], "id": "OAIKOHHJFB_1412", "locus": "GCA000209735_01403" }, { "type": "cds", "sequence": "contig_1", "start": 784121, "stop": 784507, "strand": "+", "frame": 2, "gene": null, "product": "DUF4064 domain-containing protein", "db_xrefs": [ "RefSeq:WP_013355056.1", "SO:0001217", "UniParc:UPI0001A8C3E2", "UniRef:UniRef100_C4XFN7", "UniRef:UniRef50_C4XFN7", "UniRef:UniRef90_C4XFN7" ], "nt": "ATGAATAAAAAAAGTTTTAAATCAACTTTAACTTCAATTGCTATAGCAGCAATTTTATTATTAACACTAACTATCTCATTTTTTGGATTACAAAAAAATATCCCAGAAGCAGTAATAAAAGCGACAAATAACAATTATGTGGCTGGAATTACTAACATTATTTTATTAGTTATTACTCTTGCTCTATGCATTTCTTTTATTGTTGTTGGTTCAATTCAAGCAGCAAAATTAAGTTTTCTTAAAAAAGATCGTTACATTTATGTTTTAGCAATTTTAGGAATTTTCTTTGTCCCTGTTTTCTATGTAGTAGCAGGAATTATTCTTGCTAAAAGAGATTCTATTGTTAATGAATTAGTTGATGAAGAAAATGAAGCAATTGAAGTTTAA", "aa": "MNKKSFKSTLTSIAIAAILLLTLTISFFGLQKNIPEAVIKATNNNYVAGITNIILLVITLALCISFIVVGSIQAAKLSFLKKDRYIYVLAILGIFFVPVFYVVAGIILAKRDSIVNELVDEENEAIEV", "aa_hexdigest": "fb0c22528d5377771443af7c318b9197", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C3E2", "ncbi_nrp_id": "WP_013355056.1", "uniref100_id": "UniRef100_C4XFN7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C3E2", "RefSeq:WP_013355056.1", "UniRef:UniRef100_C4XFN7" ] }, "ips": { "uniref100_id": "UniRef100_C4XFN7", "uniref90_id": "UniRef90_C4XFN7" }, "psc": { "uniref90_id": "UniRef90_C4XFN7", "product": "DUF4064 domain-containing protein", "uniref50_id": "UniRef50_C4XFN7" }, "pscc": { "uniref50_id": "UniRef50_C4XFN7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFN7" ], "product": "DUF4064 domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_1413", "locus": "GCA000209735_01404" }, { "type": "tRNA", "sequence": "contig_1", "start": 784809, "stop": 784884, "strand": "+", "gene": "trnK", "product": "tRNA-Lys(ctt)", "amino_acid": "Lys", "anti_codon": "ctt", "score": 75.9, "nt": "GCACCGTTAGCTCAGTCGGTAGAGCAGCTGGCTCTTAACCAGCGGGTCACAGGTTCGAACCCTGCACGGTGTACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 784842, 784844 ], "id": "OAIKOHHJFB_16", "locus": "GCA000209735_01405" }, { "type": "cds", "sequence": "contig_1", "start": 784965, "stop": 785222, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTATTGATCGTCTTGAAAATCTTTTCTACAAAGAAAAAAGAAGAAGACCAATTGTTGTGGCAACTATTCTTTTTACAGATGAAGAAAATGAACCAGCATTTGCAACTTCTAAAATTAAATTTAATGTTAAGGGTACAAAAGAAGATGAGAAAAGCAAAAAAGCTACTGCTGCTCTTAAAGAAGCAAAATCAGAAATGTTTGGTGAAAACAGTGAAATGTCAGAAGACATTCATGATGAAGAAGACGAAGAATAA", "aa": "MIIDRLENLFYKEKRRRPIVVATILFTDEENEPAFATSKIKFNVKGTKEDEKSKKATAALKEAKSEMFGENSEMSEDIHDEEDEE", "aa_hexdigest": "3f4bd52e31ced8ad13e37a6918a462b8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9870.935000000003, "isoelectric_point": 4.824345970153809 }, "id": "OAIKOHHJFB_1414", "locus": "GCA000209735_01406" }, { "type": "cds", "sequence": "contig_1", "start": 785244, "stop": 786341, "strand": "-", "frame": 3, "gene": null, "product": "Ribonuclease J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KH27", "UniRef:UniRef90_C4XFN8" ], "nt": "ATGTGTACCGGTGACTTTAGATTTGATTACAACCCTATTGGTGAAATTTATACTGATTTTGCTCGTTTAGATCAAATTGGAAAAGAAGGCCTAACTGTTCTTTTGTCAGACTCAACTAATGCTATGCGTCCTTTCCACTCGCCTAGTGAAAATGACATCTTAACAGATATTGAAAAATACATGCGTCAAGCTACAAGAAAAACTATTATTACAGCTTTTGCTTCAAACTTAACTAGAGTTACAGCAATTATTGAGTTAGCTGTTAAACTTGGCAAAAAAGTTGCAGCTTTTGGTCGTTCAATGGTTCAAGGAATCAAAATTGGACGTAAATTAGGTTACATTAAAGCGCCTGCAAGTGTTTTTGTTGATAAAAAAGATGTATCAAAAGTTCCTGACAAAGATCTTGTTATTCTTACAACAGGTTCACAAGGTGAACAATTGGCTGCTCTTTCAAGAATGTCTTATGGTAAACACCAAAGCGTAAAAATTGAAAAAGGTGACATGATTATCTTTTCATCAAGTCCAATTCCAGGGAATAGAATGGTTATTGAACTTTTAGTTAATAGACTTTCAAAACTTGGAGCTATTATTAAAGAAAATGGTGTTGATGGTTACTTGCATACCTCAGGTCATGCTTACAAACACGAACATGATAAAATTTTCCAACTTACTAAACCAAAATACTTTTTACCTTACCATGGTGAATTCAGAATGAGTATAGTTCATGGCCAAAGCGCTATTAGAAATGGAGTTAAACCAGAAAATATAATGATTCCTGAAATTGGTCGTGTTTACAACATGATTAATGAAGAAATTACACAAACAGATGAAAAAATAGATTGTGGTCCTATTTATATTGATGGACACACAACCTTGAATTTAAGTGCAAATATACTTAAGGAAAGAGCTAAATTAGCTGATAGCGGTTTTGTAAATATGATCCTAAATATTAACAAAAAAACTAATTCAATAGTTGGCCGTCCAAGTTTAATTAGTCGTGGGAGCTTCTATGTAAAAACTTCGCTTGAATTAGTTGAAGAAGCTAAAAGAATTGCTCATGGTGCTGCTCTTTTCTATATTAAAAATACACCTGATTGA", "aa": "MCTGDFRFDYNPIGEIYTDFARLDQIGKEGLTVLLSDSTNAMRPFHSPSENDILTDIEKYMRQATRKTIITAFASNLTRVTAIIELAVKLGKKVAAFGRSMVQGIKIGRKLGYIKAPASVFVDKKDVSKVPDKDLVILTTGSQGEQLAALSRMSYGKHQSVKIEKGDMIIFSSSPIPGNRMVIELLVNRLSKLGAIIKENGVDGYLHTSGHAYKHEHDKIFQLTKPKYFLPYHGEFRMSIVHGQSAIRNGVKPENIMIPEIGRVYNMINEEITQTDEKIDCGPIYIDGHTTLNLSANILKERAKLADSGFVNMILNINKKTNSIVGRPSLISRGSFYVKTSLELVEEAKRIAHGAALFYIKNTPD", "aa_hexdigest": "a545fda8d8fe4ce8f64bce770d4f7502", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 784965, "stop": 786641, "observations": { "mutation": [ 785246, 786407 ], "stop": [ 785246, 786407 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFN8", "query_cov": 1.0, "subject_cov": 0.5667701863354038, "identity": 1.0, "score": 716.0, "evalue": 3.3e-256, "gene_start": 1, "gene_end": 365, "reference_start": 187, "reference_end": 551, "reference_sequence": "MYFYIFIIILHNRSIKVLLAVFLNIKKGDQNMNPTRLIPIGGVQEIGKAALIVEYKNQIFIIDAGIKFADTALTGIKGIIPDYTYLAERYKNIEGLFITHGHEDHIGGVIHLMKQVAVKKIFAPRIAIQYLKLKFDENKIKNKVEFIEIEKSAVHTFGECSVDFWTAQHSIPDAFGIRIKTPNGSIMCTGDFRFDYNPIGEIYTDFARLDQIGKEGLTVLLSDSTNAMRPFHSPSENDILTDIEKYMRQATRKTIITAFASNLTRVTAIIELAVKLGKKVAAFGRSMVQGIKIGRKLGYIKAPASVFVDKKDVSKVPDKDLVILTTGSQGEQLAALSRMSYGKHQSVKIEKGDMIIFSSSPIPGNRMVIELLVNRLSKLGAIIKENGVDGYLHTSGHAYKHEHDKIFQLTKPKYFLPYHGEFRMSIVHGQSAIRNGVKPENIMIPEIGRVYNMINEEITQTDEKIDCGPIYIDGHTTLNLSANILKERAKLADSGFVNMILNINKKTNSIVGRPSLISRGSFYVKTSLELVEEAKRIAHGAALFYIKNTPDWNIVDLKQLIIDRLENLFYKEKRRRPIVVATILFTDEENEPAFATSKIKFNVKGTKEDEKSKKATAALKEAKSEMFGENSEMSEDIHDEEDEE" }, "paralog": false, "identity": 0.996415770609319, "score": 1092.0, "evalue": 0.0, "description": "Internal stop codon at 785246, 786407. Nonsense mutation around 785246, 786407.", "psc": { "uniref90_id": "UniRef90_C4XFN8", "product": "Ribonuclease J", "uniref50_id": "UniRef50_Q6KH27", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFN8", "UniRef:UniRef50_Q6KH27" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KH27", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KH27" ], "product": "Ribonuclease J" } }, "id": "OAIKOHHJFB_1415", "locus": "GCA000209735_01407" }, { "type": "cds", "sequence": "contig_1", "start": 786405, "stop": 786806, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00753.33", "PFAM:PF12706.13" ], "nt": "ATGAATCCTACAAGATTAATACCTATTGGTGGAGTTCAAGAAATTGGTAAAGCTGCACTAATAGTTGAATACAAAAACCAAATATTTATTATTGATGCAGGTATCAAATTTGCTGATACAGCTTTAACTGGTATTAAAGGTATTATTCCTGACTATACATATTTGGCAGAAAGATATAAAAATATTGAAGGTCTTTTTATTACTCATGGTCACGAAGACCACATTGGTGGTGTTATTCATTTAATGAAACAAGTAGCAGTTAAAAAGATTTTTGCACCTAGAATTGCTATTCAATATTTGAAATTAAAATTCGATGAAAATAAAATTAAAAACAAGGTTGAATTTATTGAAATTGAAAAAAGTGCTGTACACACTTTTGGCGAATGTTCAGTAGATTTTTGA", "aa": "MNPTRLIPIGGVQEIGKAALIVEYKNQIFIIDAGIKFADTALTGIKGIIPDYTYLAERYKNIEGLFITHGHEDHIGGVIHLMKQVAVKKIFAPRIAIQYLKLKFDENKIKNKVEFIEIEKSAVHTFGECSVDF", "aa_hexdigest": "e42671c62ea5b72bd2352300067aefc8", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00753.33", "name": "Lactamase_B", "length": 78, "aa_cov": 0.5864661654135338, "hmm_cov": 0.9102564102564102, "evalue": 4.0207985760435613e-10, "score": 36.02237319946289, "start": 19, "stop": 96 }, { "id": "PF12706.13", "name": "Lactamase_B_2", "length": 80, "aa_cov": 0.5714285714285714, "hmm_cov": 0.9875, "evalue": 9.05261594335983e-09, "score": 31.275922775268555, "start": 55, "stop": 130 } ], "seq_stats": { "molecular_weight": 14972.367099999985, "isoelectric_point": 7.844658088684083 }, "id": "OAIKOHHJFB_1416", "locus": "GCA000209735_01408" }, { "type": "cds", "sequence": "contig_1", "start": 787155, "stop": 787421, "strand": "+", "frame": 3, "gene": null, "product": "YobI-like P-loop NTPase domain-containing protein", "db_xrefs": [ "RefSeq:WP_013527198.1", "SO:0001217", "UniParc:UPI0001F5998B", "UniRef:UniRef100_A0AB32XDD0", "UniRef:UniRef50_C4XFP0", "UniRef:UniRef90_C4XFP0" ], "nt": "ATGTCACAAAATAATAATTCTTCTTCTTTTACAAATCACCAATGTATTAGAAATGGAAACATCCAAAATAAAGTTCAAAATGAAGAACATATTATTGAAACTATAGAGCTTTATTCAACTGAAGACATGAAAAATTCTAATGCTTTCAAGTTATATAAAAAATCACTTGATTTTGCAATGAGTAACAACAATGACAAAGTGAATAATATTGCTATTATAGGTTCTCAAGATGGACAAAATCAAAATGTTATTGAAACATATAAATAA", "aa": "MSQNNNSSSFTNHQCIRNGNIQNKVQNEEHIIETIELYSTEDMKNSNAFKLYKKSLDFAMSNNNDKVNNIAIIGSQDGQNQNVIETYK", "aa_hexdigest": "8f35698b426c44eb60d180eea03e8e97", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001F5998B", "ncbi_nrp_id": "WP_013527198.1", "uniref100_id": "UniRef100_A0AB32XDD0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5998B", "RefSeq:WP_013527198.1", "UniRef:UniRef100_A0AB32XDD0" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XDD0", "uniref90_id": "UniRef90_C4XFP0" }, "psc": { "uniref90_id": "UniRef90_C4XFP0", "product": "YobI-like P-loop NTPase domain-containing protein", "uniref50_id": "UniRef50_C4XFP0" }, "pscc": { "uniref50_id": "UniRef50_C4XFP0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFP0" ], "product": "YobI-like P-loop NTPase domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_1417", "locus": "GCA000209735_01409" }, { "type": "cds", "sequence": "contig_1", "start": 787722, "stop": 788234, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAGTTGGATTCTTAGCAATTGCTATTTATTATGGTAGAAACTATTTATTAGACTTAAAAAAATCAAAAAATATAGTAGTTCAAGAAACTAAAGAAATAGTAGTTATTGAAGATAAAGAAAAACTTGTTAACTTAATTGACAAATCTGGCTTAGATGCTTTGATTATAGAAGATTTAGGCGAATCTAAAGATTATCAATTAATCAAAAGAATTAAAGATATTAACACACTTGTAAACAAAAGAAGACAAAAAAGAATTGAAAAAATTTTAGCTAAACTTGAAAATAAAAAAGAACAAATTGCTAAATTTTATGCATCAAAATACTATGTTAAAAAATGTAAAGCAATTAAATTCTTCTATGTAATTGACAACACACTTGCATCACTAGAAGACAAAGGTAATATTTTTGATTTTTCATTAACTGTTTTACCAATTAGCAAAGAATACAAAAAAGAACAAATCAATAAATTGTTATCACAATCAAATTTAAGTGTTAAAGAATTAAGCTGA", "aa": "MIVGFLAIAIYYGRNYLLDLKKSKNIVVQETKEIVVIEDKEKLVNLIDKSGLDALIIEDLGESKDYQLIKRIKDINTLVNKRRQKRIEKILAKLENKKEQIAKFYASKYYVKKCKAIKFFYVIDNTLASLEDKGNIFDFSLTVLPISKEYKKEQINKLLSQSNLSVKELS", "aa_hexdigest": "f535557940026773864f16059ccecbb5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19756.0293, "isoelectric_point": 9.457657051086429 }, "id": "OAIKOHHJFB_1418", "locus": "GCA000209735_01410" }, { "type": "cds", "sequence": "contig_1", "start": 788289, "stop": 788771, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACTCAGATATCCATAACTTCAGAAGCACTAACACAATGGGTGATGATATTGCAGAAGAATTCAAAAAAGATTACAACAATAAATTTGAAGAAATTGTTAAATATGTAAACAGTTTATTGCAAAATAATGTATTTAGCAAAATCAAAAATCAAAACAATTTAGCTTTAAATGCATTCAAAGCTTTAAAACAAACATTAGGTGAAAACAACAAGATAGCTCAAGAATTAGCTTATGCAGGTTGTTCAATTCACTATCAAGAAATTGGTTTTGATCCTTACATTGAAAGTCAATTAGATTTATTAAGAAATAATTCAGATCTTGAAATATTCTTTAAAACAAAAAAACCTAATGAAAGTAGATATTTAATTACTTATGTTCGTAAAGCATTGAAAAACAATAGAAGTAAACTATTTGCATTCTTAATTTACAAAAACTTATTCTCACATGAATACAGCAACCTATTAGAAGAAAAAGGTTGA", "aa": "MYSDIHNFRSTNTMGDDIAEEFKKDYNNKFEEIVKYVNSLLQNNVFSKIKNQNNLALNAFKALKQTLGENNKIAQELAYAGCSIHYQEIGFDPYIESQLDLLRNNSDLEIFFKTKKPNESRYLITYVRKALKNNRSKLFAFLIYKNLFSHEYSNLLEEKG", "aa_hexdigest": "d930a71b45af3f18d3eb8310c2a904c5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18801.042700000005, "isoelectric_point": 8.48785915374756 }, "id": "OAIKOHHJFB_1419", "locus": "GCA000209735_01411" }, { "type": "cds", "sequence": "contig_1", "start": 788826, "stop": 788972, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAAATGAAAGCAAAGAAAAAGTATTTGCCAACATTAAATTAGATGGTAAAGCAATTACAGTTAAATCACTTGCATATTTATTAACCTTTAATTTATGTTCACAATTAAACTTAGATATGAATTTAACTGAATCGTTCTACTGA", "aa": "MENESKEKVFANIKLDGKAITVKSLAYLLTFNLCSQLNLDMNLTESFY", "aa_hexdigest": "0c204871d5679cf8e28c8693b158fae8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5489.296399999999, "isoelectric_point": 5.05215663909912 }, "id": "OAIKOHHJFB_1420", "locus": "GCA000209735_01412" }, { "type": "cds", "sequence": "contig_1", "start": 789057, "stop": 789830, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACTTCACACAAGATGAATACAACAAAGTTGAATCTAATAAAAATATCATTAATTTTGAAAAAGCAAGAGAAGAAATTACTGAGAAATATCAAATTGAACATCTTGAATTAATTGAGCAATTATTCAAAGAATCATACTTAGGTTTAGACTGTCAATATTACATGAATGATAGTCATCAACTTTCAAGTGAAATCAAATTAGATAATTCAGATGATGATGAAAATGGTGGCGAAAAAGAAGTTGACTTAAATGTCGAAGTTCAAAATGTTGATAATCTTGAACAATTACAAGCAGAAGCTCAAAAAGTTGTTGATTTAGAAAACTTGGCTAACTTTGAAGAAGCTGAAATTGAAGAAAAGAAAAATCAAAAGGTTGAAACTCAAAAGATAGAAGAAGATGCAAAAGAAACAATTACAGAAATTAAGGAAGAAGTTGTTCCTGAAACTAAAGTTGAAGAACAAAATATTAAAGTTGAAGAAGTTAAAAATCCTTCTGCACAACAAGCTCAACCAAATCAACCTAAATTCAATTTAAATAAACCTCAAGATCAATACCAACAAACTTCTAGACTTCGCCAAGTAAATCCAAATATGTATCAAAATGTTCAATATCCAAACCAATATCAAAACTTTCCAAATAAAGGCTATGTTGATCCAAGATTTGCACAATATCCTCCTAGACAAAACCCATATTATCCAAACCAAAATTATACAAACATAAATGTAGCTCAAAGACAACCACAAAATCCTAATAATCCTATAAAACAATAG", "aa": "MDFTQDEYNKVESNKNIINFEKAREEITEKYQIEHLELIEQLFKESYLGLDCQYYMNDSHQLSSEIKLDNSDDDENGGEKEVDLNVEVQNVDNLEQLQAEAQKVVDLENLANFEEAEIEEKKNQKVETQKIEEDAKETITEIKEEVVPETKVEEQNIKVEEVKNPSAQQAQPNQPKFNLNKPQDQYQQTSRLRQVNPNMYQNVQYPNQYQNFPNKGYVDPRFAQYPPRQNPYYPNQNYTNINVAQRQPQNPNNPIKQ", "aa_hexdigest": "1458df4c6c86c3b26ebe3a48c7d19f9c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 30352.7891, "isoelectric_point": 4.50729808807373 }, "id": "OAIKOHHJFB_1421", "locus": "GCA000209735_01413" }, { "type": "cds", "sequence": "contig_1", "start": 790026, "stop": 790490, "strand": "-", "frame": 3, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFP1", "UniRef:UniRef90_C4XFP1" ], "nt": "ATGTCAAATTTAAAACCTGAAGAAGCAAAAGAAAAAGTAAATTTCTTATTAAAAAAATATCACTTTGATGGACCTGAATTTGAAAAATCAGCAGAGCAACTTTCATCAGGCCAAAAGAAAACAATTTTATTAATGCAAGCTTTATTAAATGATCCTGAAATTTTAATTATGGATGAACCTGCTGCTAATTTAGACCCAAGTGCTAGAGTGATATTTTATGAACAAATAAAAGAATTAAGTTTACAAGGAAAAACTATATTTATTTCTAGTCATATTCTTCTAGAACTTGAAAGATATATCGATTCTTTTACGGTGCTTGCTAAGGGCAAAGTACTAGACAATGGAACAATAAAAGAAAAATTGCACAATGCAAAATATAACAAAAAAATAAATACAAATGATAACAATAAAATAGTGGAATATTTAATTAAAAACCGAATTGATTTTATTGAAAAACATGATTGA", "aa": "MSNLKPEEAKEKVNFLLKKYHFDGPEFEKSAEQLSSGQKKTILLMQALLNDPEILIMDEPAANLDPSARVIFYEQIKELSLQGKTIFISSHILLELERYIDSFTVLAKGKVLDNGTIKEKLHNAKYNKKINTNDNNKIVEYLIKNRIDFIEKHD", "aa_hexdigest": "ce46563bf145cbb5fce075b9339ef461", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 789888, "stop": 790790, "observations": { "mutation": [ 790028 ], "stop": [ 790028 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFP1", "query_cov": 1.0, "subject_cov": 0.4489795918367347, "identity": 1.0, "score": 295.0, "evalue": 2.66e-98, "gene_start": 1, "gene_end": 154, "reference_start": 144, "reference_end": 297, "reference_sequence": "MMKTLVKKSKWRIYLEKRANYKATIKRAKNYEYQQPQEDVAISIQHLHKFFKLGHGDFKTALDDVCFDVKKGSFHGFIGDNGAGKTTTIRSILGFYPSKIGKIFINGIDSTNKKAKSFIGYIPEVSLFPRKVKVKEYLLAFAEMSNLKPEEAKEKVNFLLKKYHFDGPEFEKSAEQLSSGQKKTILLMQALLNDPEILIMDEPAANLDPSARVIFYEQIKELSLQGKTIFISSHILLELERYIDSFTVLAKGKVLDNGTIKEKLHNAKYNKKINTNDNNKIVEYLIKNRIDFIEKHDWINAKLDEEQVNKFLQYCLKNNIQIYNLADNKLSLNELYFNAENVE" }, "paralog": false, "identity": 0.9933333333333333, "score": 557.0, "evalue": 4.52e-204, "description": "Internal stop codon at 790028. Nonsense mutation around 790028.", "psc": { "uniref90_id": "UniRef90_C4XFP1", "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_C4XFP1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFP1", "UniRef:UniRef50_C4XFP1" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFP1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFP1" ], "product": "ABC transporter domain-containing protein" } }, "id": "OAIKOHHJFB_1422", "locus": "GCA000209735_01414" }, { "type": "cds", "sequence": "contig_1", "start": 790946, "stop": 791176, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTCTTATGTATGGTTTAAATAAAGAACAATATGTTTTAAGTTATCAAGCTTCACCTAATAATTTGGATGAATTAAATGAATTAGACAATGCAGGTGGTATTGAATTAATTAAATTAACACCACTTGAAAGAGATTTAATTGTTGTTACAAGAAAAGATCACATGACAGTTGGCGGATCAATAATTTTATCAATGTTAGCTGCTTTTGCTATGATGGCTGGTGGTTGA", "aa": "MTLMYGLNKEQYVLSYQASPNNLDELNELDNAGGIELIKLTPLERDLIVVTRKDHMTVGGSIILSMLAAFAMMAGG", "aa_hexdigest": "3da7af128e9fc4b9501d01fd990d5ed1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8301.588199999998, "isoelectric_point": 4.597785758972168 }, "id": "OAIKOHHJFB_1423", "locus": "GCA000209735_01415" }, { "type": "cds", "sequence": "contig_1", "start": 791381, "stop": 791620, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGATAAGTTAAAACTTTTTGAATCAATATTTTTAGATAAATTATATGAAAATATTCAAGAGATCAAATTTAACAAATTACCTTATGTTATAGATTACGAGGGCAAAAAGGTATTAGTCTTATCAAAAGTATTTCAAGACTTTAACAAAATATTAAGCTATGTTGAAGAACATCAAAAAGATAAATACAAAAACTTAAAAATTAAAACGTATATTGCAACATATTATCCACAATGA", "aa": "MIDKLKLFESIFLDKLYENIQEIKFNKLPYVIDYEGKKVLVLSKVFQDFNKILSYVEEHQKDKYKNLKIKTYIATYYPQ", "aa_hexdigest": "5d26c0ca6e516fa7d0391693782ee8a2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9646.199099999994, "isoelectric_point": 9.002639198303225 }, "id": "OAIKOHHJFB_1424", "locus": "GCA000209735_01416" }, { "type": "cds", "sequence": "contig_1", "start": 791978, "stop": 792172, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTTCTAAAGAAAAAGGCAACAAGAAATTCCTTGAAGAATTAAAAGGCAGATTCAATGAAAATCAACCTATCTTGATGAGTAGATACAACGATGACTTAAAAAATACTTTATTAAATATTACAGAAGATTCAGAAGATATCACAAAATATATTGACAATACTCATAAGTCAGTTTATAACTATTTATATTGA", "aa": "MPSKEKGNKKFLEELKGRFNENQPILMSRYNDDLKNTLLNITEDSEDITKYIDNTHKSVYNYLY", "aa_hexdigest": "5f242073680cef39c575dfd506138f65", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7661.503400000002, "isoelectric_point": 5.727744865417481 }, "id": "OAIKOHHJFB_1425", "locus": "GCA000209735_01417" }, { "type": "cds", "sequence": "contig_1", "start": 792404, "stop": 792655, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAACTTCTTAACAAATATTAACCATTTTTCAATTTTTATGATTTTTATTGTTGCCACTTTTGTGTGTCAAACTGTATTTTATAAATACAAAAGAGAAGGTGTGGATTACATTTTATATTCAAAACCTTTAAGTAGAACTCAAATATATTTTGCTAATATTTTTGTATCTGCAATTGGTCTATTATTTTCAATAGCAATTATCGAATTAGGTTATTTTATAAGCAGATTGGTTTATCCAACAATTATTTGA", "aa": "MNFLTNINHFSIFMIFIVATFVCQTVFYKYKREGVDYILYSKPLSRTQIYFANIFVSAIGLLFSIAIIELGYFISRLVYPTII", "aa_hexdigest": "e4f057daf05297889f7b33a30b97688e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9722.472899999993, "isoelectric_point": 9.206359291076659 }, "id": "OAIKOHHJFB_1426", "locus": "GCA000209735_01418" }, { "type": "cds", "sequence": "contig_1", "start": 792843, "stop": 792974, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTTATGTAATTGCTATGAAAAACTCTGTTCCTGAATTTAAAGATAAAGCAGATTTTATTAGTCCTTTTACAAATAAAAAAGGTGGTTTATATAAAACTTTAGAAATGTTACTTACAAAGTTTGAATAA", "aa": "MGYVIAMKNSVPEFKDKADFISPFTNKKGGLYKTLEMLLTKFE", "aa_hexdigest": "eff5f802b51b4971c1ac375fb2db53a7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4931.786999999999, "isoelectric_point": 9.100824546813964 }, "id": "OAIKOHHJFB_1427", "locus": "GCA000209735_01419" }, { "type": "cds", "sequence": "contig_1", "start": 793149, "stop": 793349, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAATTATTATTAACTCAAACTTTAAAAATGGTTTGTACTCGAATTCATTTCTTTTTAAAGTTGCTTCAATTTTCAAAAAAGGCAAATGCTACAAACTAAAAGAATTTAATAAAGAAACTGAAGTATTTAAGTTACTTTTTCTTAATTTGTCAAAGAAAAAACTTCATTTATTTGCTGAAGAACTTAAAAAATTATGA", "aa": "MTIIINSNFKNGLYSNSFLFKVASIFKKGKCYKLKEFNKETEVFKLLFLNLSKKKLHLFAEELKKL", "aa_hexdigest": "04644b2c10858d67879e920fa9cb07b1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7807.306799999996, "isoelectric_point": 9.903262519836424 }, "id": "OAIKOHHJFB_1428", "locus": "GCA000209735_01420" }, { "type": "cds", "sequence": "contig_1", "start": 793449, "stop": 793679, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGACTAAAGAATTAAAAAAACCTAAAATAATTTTTATCGATTTGGATGGTACAACAATTGATAAAAGTAGCAAATTTAAAAAAGATATCAGTGAAATTAACCAAAAAGGTTTAGCTAAACTTAAAGAAAAATATGGCTTAGAAATTTTTGTTTCAACTGGCCGCGCAGTAGTTGAAAAAACAAAAATGTTGGCTAGAAAATGTGGCAATGAAAATAATTTTGTTGCTTGA", "aa": "MTKELKKPKIIFIDLDGTTIDKSSKFKKDISEINQKGLAKLKEKYGLEIFVSTGRAVVEKTKMLARKCGNENNFVA", "aa_hexdigest": "e200c3365709a433716dadbf93c62d6b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 55, "aa_cov": 0.7236842105263158, "hmm_cov": 0.9090909090909091, "evalue": 1.3357487770478844e-07, "score": 27.63774871826172, "start": 11, "stop": 65 } ], "seq_stats": { "molecular_weight": 8578.032499999998, "isoelectric_point": 9.678396797180177 }, "id": "OAIKOHHJFB_1429", "locus": "GCA000209735_01421" }, { "type": "cds", "sequence": "contig_1", "start": 793792, "stop": 794052, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFP4", "UniRef:UniRef90_C4XFP4" ], "nt": "ATGATTTTAAACTCAGTATTTTATAAAGCAAATAACCCATTTTACGAACAAGGAACACACAAATTAAATGCTCCTTATTTAGCTTTATTTATTATTGGATTATCACTAATAGTAATTGGAATTACATGCTTTTTCTTTTATCCTAAAGCAAAAGACAAGGTTTATTTGTATAAAGAAAAACAAATGGAAGAATATAAAAAAAATAATCCAAAATCTAAAGTAACGAATTATGAAGCAACAGGAATGTATTTACCAGCTTGA", "aa": "MILNSVFYKANNPFYEQGTHKLNAPYLALFIIGLSLIVIGITCFFFYPKAKDKVYLYKEKQMEEYKKNNPKSKVTNYEATGMYLPA", "aa_hexdigest": "2af4347964a77378f824706534822d7e", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 793792, "stop": 794148, "observations": { "mutation": [ 794050 ], "stop": [ 794050 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFP4", "query_cov": 1.0, "subject_cov": 0.7288135593220338, "identity": 1.0, "score": 176.0, "evalue": 1.57e-55, "gene_start": 1, "gene_end": 86, "reference_start": 1, "reference_end": 86, "reference_sequence": "MILNSVFYKANNPFYEQGTHKLNAPYLALFIIGLSLIVIGITCFFFYPKAKDKVYLYKEKQMEEYKKNNPKSKVTNYEATGMYLPAWERIKLFAPLFFGILFVVVGITMIVGKTISTL" }, "paralog": false, "identity": 0.9915254237288136, "score": 236.0, "evalue": 1.25e-81, "description": "Internal stop codon at 794050. Nonsense mutation around 794050.", "psc": { "uniref90_id": "UniRef90_C4XFP4", "uniref50_id": "UniRef50_C4XFP4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFP4", "UniRef:UniRef50_C4XFP4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFP4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFP4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10015.642899999993, "isoelectric_point": 9.301836967468262 }, "id": "OAIKOHHJFB_1430", "locus": "GCA000209735_01422" }, { "type": "cds", "sequence": "contig_1", "start": 794205, "stop": 794363, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00719.24" ], "nt": "ATGAGTAATATTATTGAAATGAAAGTTGAAATTCAAAAGAATTCAAACATAAAATACGAATATGATCGTGCTGACGGAAATATTCATGTAGACCGTATTTTGAGAGGAGATTTTGTCTACCCATGTAATTATGGCTTTATTCCTACAGCTTTAGATTGA", "aa": "MSNIIEMKVEIQKNSNIKYEYDRADGNIHVDRILRGDFVYPCNYGFIPTALD", "aa_hexdigest": "09b23b3bc4c5ae2db9338f44658ea512", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00719.24", "name": "Pyrophosphatase", "length": 44, "aa_cov": 0.8461538461538461, "hmm_cov": 1.0, "evalue": 2.2735238251870228e-14, "score": 49.5887451171875, "start": 8, "stop": 51 } ], "seq_stats": { "molecular_weight": 6080.8351, "isoelectric_point": 5.1192834854125975 }, "id": "OAIKOHHJFB_1431", "locus": "GCA000209735_01423" }, { "type": "cds", "sequence": "contig_1", "start": 794448, "stop": 794597, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00719.24" ], "nt": "ATGAAAATGATTGACTCAGGAGAAACAGATACAAAATTAATTGCTGTTCATGCTGATGATTATCGTTTAGATGGTATTAAAAAATTAGAAGACTTACCTGAACCATTTTTAATGCACATTGAAACATTCTTTAGCAACTATAAAAATTGA", "aa": "MKMIDSGETDTKLIAVHADDYRLDGIKKLEDLPEPFLMHIETFFSNYKN", "aa_hexdigest": "8655a983f95376eb1fa7e1c087a748f5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00719.24", "name": "Pyrophosphatase", "length": 49, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 7.812135606195277e-15, "score": 51.09521484375, "start": 1, "stop": 49 } ], "seq_stats": { "molecular_weight": 5731.486999999998, "isoelectric_point": 4.8155927658081055 }, "id": "OAIKOHHJFB_1432", "locus": "GCA000209735_01424" }, { "type": "cds", "sequence": "contig_1", "start": 795254, "stop": 795421, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAATTAAACAATGATGTTTATGATAAATTTGGTTTGCATGAAAACCGTTATAAAAAATATTTAGTTGCTTACAAAAATTTTATAAATTTATATATTGATAATGAAAATAGCAGAGCTTATATTTTTGATCTTTTAGCTCCAAAACATAATAATTTTTGTTCCTGA", "aa": "MQLNNDVYDKFGLHENRYKKYLVAYKNFINLYIDNENSRAYIFDLLAPKHNNFCS", "aa_hexdigest": "48f75f87877629d512ef708cbdd1566c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6679.4655, "isoelectric_point": 7.863096046447754 }, "id": "OAIKOHHJFB_1433", "locus": "GCA000209735_01425" }, { "type": "cds", "sequence": "contig_1", "start": 795506, "stop": 796000, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAGAAACTTTATTGATAGAAACAAAAAGGATCTTGAATTAGTTTTTGATTTAAAAGATGCTAAACAAATTAATATTAATAATTTAAAAATGTATACAGAAAATATTAACTCAGTTTTAAACAAACAATTGAATGTTGAACATTTTAGAAAATCATTAGTTAAAAATGGTGTTAATGTTGCTCCTCTTATCAAATATTATTATGGCACGGATAAATATAACAGCTTAAGAAATTTAGTTTATGATCTAACGGGCGGTTCAAACTATTTTAATGATTTTTGTTTTAAAGGAAACCACTATAAAAATTCAACATCTGTTACATTTTATCAACCAGAAAATAATGTAATAAACATTAAAATAAATCGTTTAGCTTATAGAACTGAAAATGCACAAGAAGAAATTGATATCAATAAATATTTTGAAAAAATTATTTATTCTATTATTTCAAAACAAATGAATGATGAACAAAAAATTAGAGCTATTCACAATTGA", "aa": "MFRNFIDRNKKDLELVFDLKDAKQININNLKMYTENINSVLNKQLNVEHFRKSLVKNGVNVAPLIKYYYGTDKYNSLRNLVYDLTGGSNYFNDFCFKGNHYKNSTSVTFYQPENNVINIKINRLAYRTENAQEEIDINKYFEKIIYSIISKQMNDEQKIRAIHN", "aa_hexdigest": "54a25e6fd275c82fd7a801c307af3c49", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19498.898500000003, "isoelectric_point": 9.24742565155029 }, "id": "OAIKOHHJFB_1434", "locus": "GCA000209735_01426" }, { "type": "cds", "sequence": "contig_1", "start": 796595, "stop": 796813, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAGATGGTTTATTATCTCCAGGTTTTGGGGCTGGGCCTGGACCTGGTTCTGGTGTTACAGGTGTAACTGTCGTACTTGGTGTTGAAGGTATATTGCCAGTAACATCATCTCAAAAACATGATGTTGTTATAGTTGGTAGACTAACAATAGCAGTTAGAGTACCTAGTCAATTAAATATTTTATTTTTTTTCATATTTATCTCTTTCTACAATTAA", "aa": "MLDGLLSPGFGAGPGPGSGVTGVTVVLGVEGILPVTSSQKHDVVIVGRLTIAVRVPSQLNILFFFIFISFYN", "aa_hexdigest": "7b7adee789e7d8425142ec843d51200e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7473.685599999998, "isoelectric_point": 6.509280967712402 }, "id": "OAIKOHHJFB_1435", "locus": "GCA000209735_01427" }, { "type": "cds", "sequence": "contig_1", "start": 796953, "stop": 797453, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGTAAAGAAAGCTTTAAAACTTAAAAAAGAATTATTAGAATTAAATGAAAAAATTTTTAATGCTTTTGGATTACATGAGGGAAGATTTGAAAGTTATTTAAATGCTTACAAAAATTTCGTTAATATAGGTTTTGTACAATCAAATACAGGATTAACTGCTACAATAAATGGGAATCCTGAACCTACAATAAAATATATTTTAGAGAATTATATATCTCAAGGAAGTCTTCTTAATCTTAATTATAAATTTTTTAACGACATTTTAAGCAATATAGAAAAATCTGTAAAGTACTATAAAAAAGACTTAGACTTATCATTTGACATTAAAGATGCATATGAAGTTGATTTAAACAATTTGGACTTTCTAAAAAGTGCTAAAGCTGTTGATACTCTCACAAAAGAACAAGTTTTAAAAATTAGAAAAGACTTGCTATTTAAAGGAAAAATAACTTCTGATATTGCCGTAACTAATCATAATTTAGGAGATTATTTCTGA", "aa": "MLVKKALKLKKELLELNEKIFNAFGLHEGRFESYLNAYKNFVNIGFVQSNTGLTATINGNPEPTIKYILENYISQGSLLNLNYKFFNDILSNIEKSVKYYKKDLDLSFDIKDAYEVDLNNLDFLKSAKAVDTLTKEQVLKIRKDLLFKGKITSDIAVTNHNLGDYF", "aa_hexdigest": "8a39ac61662c67e5222f7cc6ab116429", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19074.695900000002, "isoelectric_point": 8.552198600769042 }, "id": "OAIKOHHJFB_1436", "locus": "GCA000209735_01428" }, { "type": "cds", "sequence": "contig_1", "start": 797691, "stop": 797870, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTGACAATAATGAATATTTGACTGATGGAGAGTTAGCTTTATCAAATAATGAACCAATACGAAGTCAATGTCGTAGTCCATATTCATATTTTACTAAACCAAAAAGAAGAATAGTTTGCGAAGGATATGCAAGAACATTTTCAAGATTTCTAACATTAATAAATATTCCTGTGTGA", "aa": "MIDNNEYLTDGELALSNNEPIRSQCRSPYSYFTKPKRRIVCEGYARTFSRFLTLINIPV", "aa_hexdigest": "e4f011fdaf9d1c7e48010e50eee049db", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6902.802399999999, "isoelectric_point": 8.736062431335448 }, "id": "OAIKOHHJFB_1437", "locus": "GCA000209735_01429" }, { "type": "cds", "sequence": "contig_1", "start": 798247, "stop": 798399, "strand": "+", "frame": 1, "gene": "rpmG", "product": "50S ribosomal protein L33", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013355046.1", "SO:0001217", "UniParc:UPI0001E3307F", "UniRef:UniRef100_C4XFP9", "UniRef:UniRef50_P35871", "UniRef:UniRef90_C4XFP9" ], "nt": "ATGCCAAGAGACGGTTTTACATTAGTTTGCGAATCATGTAAAATGGAAAACTACATTAGTAAAAAGAACAAGAAAAATACACCAGAAAAAGTTGAATTAAGCAAATATTGTTCAAAATGTAGAAAACACCAAAATCACAAAGAAAAGAAATAA", "aa": "MPRDGFTLVCESCKMENYISKKNKKNTPEKVELSKYCSKCRKHQNHKEKK", "aa_hexdigest": "4965179e4d6cbe3993b3906007accba1", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001E3307F", "ncbi_nrp_id": "WP_013355046.1", "uniref100_id": "UniRef100_C4XFP9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3307F", "RefSeq:WP_013355046.1", "UniRef:UniRef100_C4XFP9" ] }, "ips": { "uniref100_id": "UniRef100_C4XFP9", "uniref90_id": "UniRef90_C4XFP9" }, "psc": { "uniref90_id": "UniRef90_C4XFP9", "gene": "rpmG", "product": "50S ribosomal protein L33", "uniref50_id": "UniRef50_P35871", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P35871", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P35871" ], "product": "Large ribosomal subunit protein bL33" }, "genes": [ "rpmG" ], "id": "OAIKOHHJFB_1438", "locus": "GCA000209735_01430" }, { "type": "cds", "sequence": "contig_1", "start": 798717, "stop": 798941, "strand": "+", "frame": 3, "gene": null, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A661HMY4" ], "nt": "GTGCAAAATGTATATTTGCCACTTTTAATACCTGAAAAATTGTTTAACAAAGAAAAAGATCACATAGCAGGATTTAATCCAGAATTAGCAACAATTACTAAAGTTGGCGACAAGGATTTAGATGAAAAAGTATTTATTAGACCAACATCAGAAGTTTTATTTGCTGATTTATTTAAAAACTCAATTGAGTCTTACAAAGACTTACCTATGATTTATAACCAATGA", "aa": "MQNVYLPLLIPEKLFNKEKDHIAGFNPELATITKVGDKDLDEKVFIRPTSEVLFADLFKNSIESYKDLPMIYNQ", "aa_hexdigest": "22489394e1143e09c5d48d3cbf0b2e0b", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A661HMY4", "query_cov": 1.0, "subject_cov": 0.891566265060241, "identity": 0.527, "score": 87.8, "evalue": 2.74e-21, "valid": false, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "uniref50_id": "UniRef50_A0A661HMY4" }, "pscc": { "uniref50_id": "UniRef50_A0A661HMY4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A661HMY4" ], "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_1439", "locus": "GCA000209735_01431" }, { "type": "cds", "sequence": "contig_1", "start": 799062, "stop": 799967, "strand": "+", "frame": 3, "gene": "proS", "product": "proline--tRNA ligase", "db_xrefs": [ "EC:6.1.1.15", "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433", "KEGG:K01881", "SO:0001217", "UniRef:UniRef50_Q98R27", "UniRef:UniRef90_C4XFQ0" ], "nt": "ATGATTTCAACTTATTCAAAATTTTTAAAAAACTTTTTAGCAATACCAACAATTATTGGTAAGAAAACGCCAAAAGAAAAATTTGCTGGCGCATGTTCAACTTATACAGTTGAAGCAATGATGAAGGATGGTCGTGCTTTACAAGCAGGAACAAGCCATTATTTAGCTCAAAACTTTTCAAAACAATTTGAAATTCAATTCAAAACTGAAAACAATGAATTAGATTATGCTTACCAAACTTCATGGGGTGTTTCAACCCGTCTGCTTGGAGCTATTATAATGACTCATGGAGACAATAGAGGAATTATTATTCCACCTCGAGTAGCTCCAATTCAAATAGATGTTTTAGAGCTTTTTGCAAATAAAAATAAAGAAGTTCATACATACTGTGATAATCTTTACAAAGACCTAAGTAGAAAATTTAGAGTAAGACTTGATTCAAGTGACAAAGGTCCAGGATTTAAAGCCTCAAATTCTGAAATTCAAGGTGTTCCTCTTAGAATTGAAGTTGGACCTAAAGACATTGAAAATAAAAGTGTGACAATTGTTAGAAGAGACACCCTTGAAAAGATTAATATAAAACAATCAGAAGTTAAGGATCAAGTTGAAACTCTTTTAAATCAAATCCACGATAACTTGTACTTACAAGCTGAAAAAAGACTAAATGAAAATACAGTCTTTGTAGATAACTACGAAGATTTCAAAAAACAAATTAAAAATCATAAATTTGTTATAGCCCCATTTTGTTGTTTAGATGTGGCTGAAGAAATAATCAAAAATGAAACTGGCGCTTCAACTCGTTGCTTACCTAAAAAGTTTGATAAACCAAATCAAAAACACAAATGTATTTTTGAAAAATGTTGTAAAGAAACCAATAGATATGTAATCTTTGCAAGAGCTTACTAG", "aa": "MISTYSKFLKNFLAIPTIIGKKTPKEKFAGACSTYTVEAMMKDGRALQAGTSHYLAQNFSKQFEIQFKTENNELDYAYQTSWGVSTRLLGAIIMTHGDNRGIIIPPRVAPIQIDVLELFANKNKEVHTYCDNLYKDLSRKFRVRLDSSDKGPGFKASNSEIQGVPLRIEVGPKDIENKSVTIVRRDTLEKINIKQSEVKDQVETLLNQIHDNLYLQAEKRLNENTVFVDNYEDFKKQIKNHKFVIAPFCCLDVAEEIIKNETGASTRCLPKKFDKPNQKHKCIFEKCCKETNRYVIFARAY", "aa_hexdigest": "2e3c870226400edc3a969c193508d9d3", "start_type": "ATG", "rbs_motif": null, "genes": [ "proS" ], "truncated": "5-prime", "pseudogene": { "start": 798762, "stop": 799967, "observations": { "mutation": [ 798939, 798957, 799002 ], "stop": [ 798939, 798957, 799002 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFQ0", "query_cov": 1.0, "subject_cov": 0.6244813278008299, "identity": 1.0, "score": 604.0, "evalue": 1.44e-215, "gene_start": 1, "gene_end": 301, "reference_start": 182, "reference_end": 482, "reference_sequence": "MIKIMKELEKITPLEQDFAKWYTDVVKQGNLIAYGPVKGTIVFKPNSYGIWEQIQKNLNECFREKGVQNVYLPLLIPEKLFNKEKDHIAGFNPELATITKVGDKDLDEKVFIRPTSEVLFADLFKNSIESYKDLPMIYNQWANVVRWEKTTNPFLRSREFLWQEGHTCHSNPLEARKFTKGMISTYSKFLKNFLAIPTIIGKKTPKEKFAGACSTYTVEAMMKDGRALQAGTSHYLAQNFSKQFEIQFKTENNELDYAYQTSWGVSTRLLGAIIMTHGDNRGIIIPPRVAPIQIDVLELFANKNKEVHTYCDNLYKDLSRKFRVRLDSSDKGPGFKASNSEIQGVPLRIEVGPKDIENKSVTIVRRDTLEKINIKQSEVKDQVETLLNQIHDNLYLQAEKRLNENTVFVDNYEDFKKQIKNHKFVIAPFCCLDVAEEIIKNETGASTRCLPKKFDKPNQKHKCIFEKCCKETNRYVIFARAY" }, "paralog": false, "identity": 0.9925187032418953, "score": 801.0, "evalue": 7.41e-296, "description": "Internal stop codon at 798939, 798957, 799002. Nonsense mutation around 798939, 798957, 799002.", "psc": { "uniref90_id": "UniRef90_C4XFQ0", "gene": "proS", "product": "proline--tRNA ligase", "ec_ids": [ "6.1.1.15" ], "uniref50_id": "UniRef50_Q98R27", "kegg_orthology_id": "K01881", "go_ids": [ "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFQ0", "EC:6.1.1.15", "UniRef:UniRef50_Q98R27", "KEGG:K01881", "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433" ] }, "pscc": { "uniref50_id": "UniRef50_Q98R27", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98R27" ], "product": "Proline--tRNA ligase" } }, "id": "OAIKOHHJFB_1440", "locus": "GCA000209735_01432" }, { "type": "cds", "sequence": "contig_1", "start": 800009, "stop": 800281, "strand": "+", "frame": 2, "gene": null, "product": "Transposase", "db_xrefs": [ "RefSeq:WP_259637433.1", "SO:0001217", "UniParc:UPI000FF5E58A", "UniRef:UniRef100_C4XFQ1", "UniRef:UniRef50_C4XFQ1", "UniRef:UniRef90_C4XFQ1" ], "nt": "ATGACAAGTACTATTTCGCAATTTGCTTCACAATACCAAATGAGTAATTTACAAAAGTATCGTAGTGTAGAAACAATAATTAATATGGAAAAAGCCATATTCTTGAAAAATCGTCAAGAAGATTTTATAGAAAAATATTACAAGAAGAATGAAAATTCAAGATCTAATAATTACTTTTCAGACAAATTCAAAAGAGTATACAATTGCTTAAGCGGTGTAAGTCAACTGATCATTAAAAATGAGTTTATGAAACAATCTGAAAACAAGTTATGA", "aa": "MTSTISQFASQYQMSNLQKYRSVETIINMEKAIFLKNRQEDFIEKYYKKNENSRSNNYFSDKFKRVYNCLSGVSQLIIKNEFMKQSENKL", "aa_hexdigest": "de9335703414c11ffa78d0d92e3af720", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000FF5E58A", "ncbi_nrp_id": "WP_259637433.1", "uniref100_id": "UniRef100_C4XFQ1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000FF5E58A", "RefSeq:WP_259637433.1", "UniRef:UniRef100_C4XFQ1" ] }, "ips": { "uniref100_id": "UniRef100_C4XFQ1", "uniref90_id": "UniRef90_C4XFQ1" }, "psc": { "uniref90_id": "UniRef90_C4XFQ1", "product": "Transposase", "uniref50_id": "UniRef50_C4XFQ1" }, "pscc": { "uniref50_id": "UniRef50_C4XFQ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFQ1" ], "product": "Transposase" }, "genes": [], "id": "OAIKOHHJFB_1441", "locus": "GCA000209735_01433" }, { "type": "cds", "sequence": "contig_1", "start": 800537, "stop": 800857, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGGGCATTAGCCCTCCCTAAAATATTTGGCGACTTGTCGCCCTTTCTAAAATATTATTTTAATTTTCCTATTTTTTTGAAAAACAAATTTCTATTCTTGTTACTTTCTCAGTTTCTCTTTTGTTTTCCATATAATACTTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCGTACATTTCCAAAGCAGATAAATATCCAAAAATTTCTCTTGGACGATTGTTTATTTCATTTTGTACTTTTAGAATTTCTTCTTCAATAATATCATCAAAATTAGTTTTTTTTCTTAAAAAATCTTCTAACTAA", "aa": "MRALALPKIFGDLSPFLKYYFNFPIFLKNKFLFLLLSQFLFCFPYNTLSIPVEFQGFSAFNFSYISKADKYPKISLGRLFISFCTFRISSSIISSKLVFFLKKSSN", "aa_hexdigest": "920815c94b6ebd0c1000b79640b9735d", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12364.57399999998, "isoelectric_point": 9.96186428070068 }, "id": "OAIKOHHJFB_1442", "locus": "GCA000209735_01434" }, { "type": "cds", "sequence": "contig_1", "start": 801575, "stop": 801916, "strand": "+", "frame": 2, "gene": "exiS", "product": "ExiS", "db_xrefs": [ "RefSeq:WP_013355043.1", "SO:0001217", "UniParc:UPI0001E3307C", "UniRef:UniRef100_A0AB32XDB6", "UniRef:UniRef50_A0AB32XDB6", "UniRef:UniRef90_A0AB32XDB6" ], "nt": "ATGTGTAAAGTTTCAGATTACAATGTAGCAAAACAGTTAATATTGTTTTTTATTTTACAATTATTTTCAATGTTAAAAGTTCCTACCTATAAAGATTTAAATATAAATATTAAAAAGATTTTGTGGACTAAAAATTATAAAAGCTTTACAGAGTTCCAAAAGGCATATAGTGGCGTTTTTTGTGCTAAATTAGGTCATTATCTAAGTAAGCATAAAGCCAACACCAAAACAAATTTTTTAAATATGTATCAAATTATTTTTTATCAAGGTTTTATTAATTACAACAGAGAAAAGGAATTATTAGATTTATTGACTTATAAAGAAAAGTCTCAGACATATTAA", "aa": "MCKVSDYNVAKQLILFFILQLFSMLKVPTYKDLNINIKKILWTKNYKSFTEFQKAYSGVFCAKLGHYLSKHKANTKTNFLNMYQIIFYQGFINYNREKELLDLLTYKEKSQTY", "aa_hexdigest": "ca41be5796e33309c75e728d0372d953", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E3307C", "ncbi_nrp_id": "WP_013355043.1", "uniref100_id": "UniRef100_A0AB32XDB6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3307C", "RefSeq:WP_013355043.1", "UniRef:UniRef100_A0AB32XDB6" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XDB6", "uniref90_id": "UniRef90_A0AB32XDB6" }, "psc": { "uniref90_id": "UniRef90_A0AB32XDB6", "product": "ExiS", "uniref50_id": "UniRef50_A0AB32XDB6" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XDB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XDB6" ], "product": "ExiS" }, "genes": [ "exiS" ], "id": "OAIKOHHJFB_1443", "locus": "GCA000209735_01435" }, { "type": "cds", "sequence": "contig_1", "start": 802372, "stop": 802821, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTATCTACTTTACTAAATACAATTAATGTAACTAACATAACACCATCAGTCATTGTTGCATCAAATGAAAATTTAAATTCAGAGCAACAAAAAATTTTGGAATTATTTTCTCGATTTACAAACGATGAAATTGAAATTTTTAACTATATCTCAAATGATATTATCAATAACAAAAATTCAACTAAAAAAGTTATAAATTCCATTGATGAAATAAAATTAGATAAAATTTATGATTTGTTTATATCTAAAATTAGCCAATCTAGTATAAAAAATAAAGATGAATTATTAAATAAACTAAGAGACAAAAGAGAAATTTTTAAAACTGAATTAATTAAAAAAATAAAAGAACAATCTTTAACTTTTAAAAAAAGCGAAAAAAAATCAAAAATAGAAATCAATTCAATGATAAAAAGAGTCAATTCGTCTTTTGAAAATAAAGATTGA", "aa": "MKLSTLLNTINVTNITPSVIVASNENLNSEQQKILELFSRFTNDEIEIFNYISNDIINNKNSTKKVINSIDEIKLDKIYDLFISKISQSSIKNKDELLNKLRDKREIFKTELIKKIKEQSLTFKKSEKKSKIEINSMIKRVNSSFENKD", "aa_hexdigest": "f4afcd0e1f87f7841e4c727d1bb7c2e3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17375.80749999999, "isoelectric_point": 9.397314643859861 }, "id": "OAIKOHHJFB_1444", "locus": "GCA000209735_01436" }, { "type": "cds", "sequence": "contig_1", "start": 803197, "stop": 803451, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTCAAGTATTCCTATGGCATTAAATCGTGTTGCTAGAGTATATGAAATGCTTAAATTATCACAAGCTGCGCCATCAATAGCAACTATGGCTACTGGTGTTGTTGGTTCAGCATTACAAACTCTTGGATCATTTTATGATGTTTACACCGCTTCAAAAGAATTAGATTATTTTAATGAATGTGCTAAAAAGGCATCAGATTTAATTAGTGATTTTTCAAAGAAAATAAAAGATATTAAAAAAATAGGATGA", "aa": "MVSSIPMALNRVARVYEMLKLSQAAPSIATMATGVVGSALQTLGSFYDVYTASKELDYFNECAKKASDLISDFSKKIKDIKKIG", "aa_hexdigest": "739759822f8d92f287065c50fef326ad", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9127.559099999993, "isoelectric_point": 9.09850368499756 }, "id": "OAIKOHHJFB_1445", "locus": "GCA000209735_01437" }, { "type": "cds", "sequence": "contig_1", "start": 803487, "stop": 803762, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGCGTAAGATTAATAAAAGCAATTCAAAAACTAAAAAAGATAATTGAAAAAATTAAAAAATTACTCATATGTGATTATCCTAAATTATCATATAAAGAATTATTAGCTTTTTTACGCAAATATGTTCCTTTTTTAGGGTCATTAACTCTTTGTAAACTTCACAAACTTAATTCAAAATCAGAAAGCTCTAGCATTTCATTAATAGTTTTTATTTCGTTGGTTTCTAAATTTTTAAAAATAAGGTTTTTTCCACCAGTACCTCCCATATAA", "aa": "MNGVRLIKAIQKLKKIIEKIKKLLICDYPKLSYKELLAFLRKYVPFLGSLTLCKLHKLNSKSESSSISLIVFISLVSKFLKIRFFPPVPPI", "aa_hexdigest": "5f1d287fa27a157290594ec3daa0662e", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10476.840899999988, "isoelectric_point": 10.203556251525882 }, "id": "OAIKOHHJFB_1446", "locus": "GCA000209735_01438" }, { "type": "cds", "sequence": "contig_1", "start": 803824, "stop": 804030, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGAAAATTAAGAGTTCAAAATTCATTAGTAAATCATTATTGTCTGATGTCTAAAATTCCATTAATTTATGCTTTACTTGCAATCGCATTAATGCTAGAAAGCTTAGTTTTAATGGTTTTTCTACAAGTATATGAAAAGGAATCATTTTTTACAATGTTTGTAACACTTGGCATAATATTTTATGTACCATTGCCCTTGTGA", "aa": "MKRKLRVQNSLVNHYCLMSKIPLIYALLAIALMLESLVLMVFLQVYEKESFFTMFVTLGIIFYVPLPL", "aa_hexdigest": "73034ecf5c94e4b169ab768fbfd9b822", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7955.764499999995, "isoelectric_point": 9.301514625549316 }, "id": "OAIKOHHJFB_1447", "locus": "GCA000209735_01439" }, { "type": "cds", "sequence": "contig_1", "start": 804091, "stop": 804381, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFQ5", "UniRef:UniRef90_C4XFQ5" ], "nt": "ATGCTTCCAGATAGTTCTTTAATCGAAAAATCAATTGACATAAACACAGATGTTGAACAATTGTATCCATCTAAAAATAAATGTTATTTTAAAAGACAAGGTTTTAATGCACTTTATTTTTCAACTTTTAATACTCAAAAATTCAAGTCATACTCATTCGAAAAGCAAAAACTTTTTATTTATATGACTATGGTTCTCAATTACGATGATACAGCATTTGATAATAAATTTGAGCCGCTAAATATTAATATGTTCAAAAATGAAGCAAAAGCTTATAAAATTATTGAATAA", "aa": "MLPDSSLIEKSIDINTDVEQLYPSKNKCYFKRQGFNALYFSTFNTQKFKSYSFEKQKLFIYMTMVLNYDDTAFDNKFEPLNINMFKNEAKAYKIIE", "aa_hexdigest": "9461ed71d51c23faf2888eab3867f672", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 803791, "stop": 804381, "observations": { "mutation": [ 804028, 804052, 804073 ], "stop": [ 804028, 804052, 804073 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFQ5", "query_cov": 1.0, "subject_cov": 0.41739130434782606, "identity": 1.0, "score": 194.0, "evalue": 4.1e-61, "gene_start": 1, "gene_end": 96, "reference_start": 135, "reference_end": 230, "reference_sequence": "MSNFLIFSIIFFSFWIAFINLTPFIILFPKIRWYIYAFGSVNKYCMKRKLRVQNSLVNHYCLMSKIPLIYALLAIALMLESLVLMVFLQVYEKESFFTMFVTLGIIFYVPLPLWYIVVCLLWYIKQKKWRKFNNMLPDSSLIEKSIDINTDVEQLYPSKNKCYFKRQGFNALYFSTFNTQKFKSYSFEKQKLFIYMTMVLNYDDTAFDNKFEPLNINMFKNEAKAYKIIE" }, "paralog": false, "identity": 0.9846938775510204, "score": 383.0, "evalue": 5.13e-138, "description": "Internal stop codon at 804028, 804052, 804073. Nonsense mutation around 804028, 804052, 804073.", "psc": { "uniref90_id": "UniRef90_C4XFQ5", "uniref50_id": "UniRef50_C4XFQ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFQ5", "UniRef:UniRef50_C4XFQ5" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFQ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFQ5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11489.03199999999, "isoelectric_point": 7.744960594177246 }, "id": "OAIKOHHJFB_1448", "locus": "GCA000209735_01440" }, { "type": "cds", "sequence": "contig_1", "start": 804450, "stop": 804623, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTTTGTTCATAGATTCAATTTTAGTCAAACAAAATACCTTTAAAAAAATCTTATATTTTTGTCTAACAGTAAGACCTAATTGCATAAGTAATGAAATAAAACTTCAAGATTTTGTTATCAAAAACCAACAAAAATATCAATTATTCTATATGTTAGGTTCATTGGGCTGA", "aa": "MPLFIDSILVKQNTFKKILYFCLTVRPNCISNEIKLQDFVIKNQQKYQLFYMLGSLG", "aa_hexdigest": "7ab5aaceae9cb010f8c936aed4731a07", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6747.0185, "isoelectric_point": 9.343483543395994 }, "id": "OAIKOHHJFB_1449", "locus": "GCA000209735_01441" }, { "type": "cds", "sequence": "contig_1", "start": 804792, "stop": 805139, "strand": "+", "frame": 3, "gene": null, "product": "MAG0920 family protein", "db_xrefs": [ "RefSeq:WP_245525948.1", "SO:0001217", "UniParc:UPI0010051079", "UniRef:UniRef100_UPI0010051079", "UniRef:UniRef50_UPI00217F505D", "UniRef:UniRef90_UPI00217F505D" ], "nt": "TTGAAATTCAACAATAATTTAAATGACGAAAATCTTTATGAAAAGAAGATTAATTCAGAAACTAACAAAACACAATTTGATTTTTCATTAGGCCAAAAATATCACTATAACTCCCTTCCAAATGAAAAGTCGTTAGTTTCATTCTTTTTCAGATCAGTCGATTTTTATATTAAATATCCAGAAAATTTTGAAAACTTATCTCCTAAAAAGCAAAGAAAATTAGTATATGCAAATTCAATCTTCGATTTTGAAAATACTAAGCCTGATAAATTAACATATTTTGATATTAATATGTTTAAAGACACATTTATTAAATATCAAATTATCCAGAATAATAAAAATGATTAG", "aa": "MKFNNNLNDENLYEKKINSETNKTQFDFSLGQKYHYNSLPNEKSLVSFFFRSVDFYIKYPENFENLSPKKQRKLVYANSIFDFENTKPDKLTYFDINMFKDTFIKYQIIQNNKND", "aa_hexdigest": "08012eba18b2f42d4acc758c829d58d6", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0010051079", "ncbi_nrp_id": "WP_245525948.1", "uniref100_id": "UniRef100_UPI0010051079", "db_xrefs": [ "SO:0001217", "UniParc:UPI0010051079", "RefSeq:WP_245525948.1", "UniRef:UniRef100_UPI0010051079" ] }, "ips": { "uniref100_id": "UniRef100_UPI0010051079", "uniref90_id": "UniRef90_UPI00217F505D" }, "psc": { "uniref90_id": "UniRef90_UPI00217F505D", "product": "MAG0920 family protein", "uniref50_id": "UniRef50_UPI00217F505D" }, "pscc": { "uniref50_id": "UniRef50_UPI00217F505D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00217F505D" ], "product": "MAG0920 family protein" }, "genes": [], "id": "OAIKOHHJFB_1450", "locus": "GCA000209735_01442" }, { "type": "cds", "sequence": "contig_1", "start": 805284, "stop": 805418, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XD66", "UniRef:UniRef90_A0AB32XD66" ], "nt": "ATGGGAAAACATTTAAATACTGAAAAATGGATAGAAATTTTTATATTTTTTAAAGAATATTTAGACTATAAAATTACAAAGATGGAATTTAAAATGAATATTTTAGAAAGGGCGACAAGTCGCCAAATATTTTAG", "aa": "MGKHLNTEKWIEIFIFFKEYLDYKITKMEFKMNILERATSRQIF", "aa_hexdigest": "64fe2ec0c087f6ace803cb90d8364e4a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 805284, "stop": 805529, "observations": { "mutation": [ 805427 ], "stop": [ 805427 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XD66", "query_cov": 1.0, "subject_cov": 0.5432098765432098, "identity": 1.0, "score": 92.8, "evalue": 7.66e-24, "gene_start": 1, "gene_end": 44, "reference_start": 38, "reference_end": 81, "reference_sequence": "MLKINKKNTTNFVVLNQDKRIRLKSQKDLSLFVLWSFMGKHLNTEKWIEIFIFFKEYLDYKITKMEFKMNILERATSRQIF" }, "paralog": false, "identity": 0.9876543209876543, "score": 159.0, "evalue": 1.37e-52, "description": "Internal stop codon at 805427. Nonsense mutation around 805427.", "psc": { "uniref90_id": "UniRef90_A0AB32XD66", "uniref50_id": "UniRef50_A0AB32XD66", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XD66", "UniRef:UniRef50_A0AB32XD66" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB32XD66", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XD66" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5532.501499999999, "isoelectric_point": 9.157298851013184 }, "id": "OAIKOHHJFB_1451", "locus": "GCA000209735_01443" }, { "type": "cds", "sequence": "contig_1", "start": 805605, "stop": 806084, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATAATAGTAATAATATGCCTAAAGAAAATAGAAAAATAGAAAATGAAAATTTCATTACATTTTGTAAAGATATTAATGTAAAAGTGACACCTGGATTGGATAAAGTTGAATTAGACGATGTCGAATATGACAGTGTTAAAGACCAATTAAATGTGTCATTTAAATTTACAAATTCAATCAGTGCAAAGGAATTTTTTTCGTTTCTTTCTAGTTTAAAAAATAATAAAGTTTATAACATCAAAGGTAAAATTACTTTTGATATTTCACAATATAAAAAAGAAAATTTAATAGATTTTATTAACGAATTAACCTATGAAAAAGCCAAATTCATCAAATTGAGAAATTTGCCAATTGTTAAAAAACTTAAATTTAATGATCAATTTGTAGCAACAATTACAACTATTTCAGCTAAAGAATATGAAGAGTTTAACCCCGTTTTTAAAAACCTTCAAAAAACTTTAAATAAATACGGTTGA", "aa": "MYNSNNMPKENRKIENENFITFCKDINVKVTPGLDKVELDDVEYDSVKDQLNVSFKFTNSISAKEFFSFLSSLKNNKVYNIKGKITFDISQYKKENLIDFINELTYEKAKFIKLRNLPIVKKLKFNDQFVATITTISAKEYEEFNPVFKNLQKTLNKYG", "aa_hexdigest": "05d59905c7894aa0f5fe8017448e4e82", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18685.207599999998, "isoelectric_point": 9.097665596008301 }, "id": "OAIKOHHJFB_1452", "locus": "GCA000209735_01444" }, { "type": "cds", "sequence": "contig_1", "start": 806334, "stop": 806486, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTATTCTCAAGACTATGCTTTAAGTAAAAATGGTAAACACATTTACACATTTTTTCTAACCAACTATAAAGATGCTGTAGAAGTAAAAACAATAAGAACAACATTATTAGAACCTGAACAAATAAATGAGCTTGAAGACAACAAATGA", "aa": "MIYSQDYALSKNGKHIYTFFLTNYKDAVEVKTIRTTLLEPEQINELEDNK", "aa_hexdigest": "51ce6b88c245a52c9ac8810f9f25e88d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5926.615999999998, "isoelectric_point": 5.12309169769287 }, "id": "OAIKOHHJFB_1453", "locus": "GCA000209735_01445" }, { "type": "cds", "sequence": "contig_1", "start": 806643, "stop": 807362, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13482.12", "PFAM:PF00929.31" ], "nt": "ATGAACACAATGGATGGATTAATTTTTCCTAAAGACATTGTGGCTTTTGCTGAAAAATTGAATCATTCTGCTGTTGCAATTATGGACGTTGATGGTGCTCAAGGTTATCCTGAATTTTATAATGCAGCTAAAAAATCAAAAGTTAAACCTCTTTTTGGAACTGCGTTTTCAGTTATAAACAAAAGTAATAAATCTATTTTAGGAAACATACCTGAAGGCGAAATTAAAGGTCAAGAATATGTTTCATTCGATATTGAAACCACTGGACTTTGTCCTCGTTTTGCCGTAATTATTGAATATGGTTCATTGCCAATTCACTCAAACATGAAGTATGGGAGAAAAACACAATTTTTCATTAAATGTCAAGAACCAATTAAACCTTTTACAGAAAACTTGACAGGTATTACTAATGCAATGATTGAAAAAAGTGGTTTGGAATTAAAAGAAGCTTTGCAAAAAATTTATAATGACTTAAATGGCAAAATTGCTTTAGCACACAATGCTAGATTTGACTTCAACTTCTTAAAAGAAAAATTTAGACAAGCTAAAATAGAATTTCCAAATGTAACAGTAATTGACACACTTATTGTTTCAAGAATTGTTTTCCCTCAAAACAAAAAACATAAACTTGAAGATGTTGCTACTCGTGTTGGTGTTGAATATGATCCTACAGTTGCTCACCGTGGTGATTATGATGCGAAAGTTTTAGCTGAAGTTTGA", "aa": "MNTMDGLIFPKDIVAFAEKLNHSAVAIMDVDGAQGYPEFYNAAKKSKVKPLFGTAFSVINKSNKSILGNIPEGEIKGQEYVSFDIETTGLCPRFAVIIEYGSLPIHSNMKYGRKTQFFIKCQEPIKPFTENLTGITNAMIEKSGLELKEALQKIYNDLNGKIALAHNARFDFNFLKEKFRQAKIEFPNVTVIDTLIVSRIVFPQNKKHKLEDVATRVGVEYDPTVAHRGDYDAKVLAEV", "aa_hexdigest": "f621a5b29f4ee6a7dffb6ca2973db5d2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13482.12", "name": "RNase_H_2", "length": 142, "aa_cov": 0.5857740585774058, "hmm_cov": 0.8169014084507042, "evalue": 4.051584889668729e-06, "score": 22.941926956176758, "start": 83, "stop": 222 }, { "id": "PF00929.31", "name": "RNase_T", "length": 166, "aa_cov": 0.6652719665271967, "hmm_cov": 0.9819277108433735, "evalue": 1.1656539210630707e-26, "score": 90.33883666992188, "start": 81, "stop": 239 } ], "seq_stats": { "molecular_weight": 26810.60410000003, "isoelectric_point": 8.349445533752444 }, "id": "OAIKOHHJFB_1454", "locus": "GCA000209735_01446" }, { "type": "cds", "sequence": "contig_1", "start": 807372, "stop": 807728, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACGAATTAGGTAAAAAAGGTATTTTAACCTTTAATCAACTTAATTCATATATTAAAGATGATCTTTATAATAAAACCTTTTCATATGAAGTTTCAACAATAGCTAAAAACTATGAAGGTTTAAAAGAACAATATGGTTATTTAACAACAGCTTTAACTAAAAACTTATACAATGGACCTAAAACATTTATGGAAGATTTAGCAGCTCATAGTGAAAATCTTTTATTAGGTTCAGGAACACTAAAAAGTAGATTGCTTGATGTTTATTTTTATTCTTCACATGAAGAATTCATTAAAGAATTAAAACTTTATGACTATATTGAAGTGCCAGCACCTCAAGTGTTTAGCCATTGA", "aa": "MDELGKKGILTFNQLNSYIKDDLYNKTFSYEVSTIAKNYEGLKEQYGYLTTALTKNLYNGPKTFMEDLAAHSENLLLGSGTLKSRLLDVYFYSSHEEFIKELKLYDYIEVPAPQVFSH", "aa_hexdigest": "42cf8305374339e3d414d753f2e741cf", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13625.23669999999, "isoelectric_point": 5.308500480651855 }, "id": "OAIKOHHJFB_1455", "locus": "GCA000209735_01447" }, { "type": "cds", "sequence": "contig_1", "start": 807813, "stop": 808259, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTGCTACAGCTGATGTTAAGTATCTAAATTTATATGACAAAGTAGCTTATGAGGTTTTAGTTTATGCTAAAGGAATTAAAAACTCTCGTCATTACTTATTCAACTATGAACAAGCTAAAAACGGAAATATTCCAATTCCAAATCAAGACTATTTAACCACTGAAGAAATGCTTGAACAATTTGCCTTTTTAAAAGACAAACAATTAATTGAAGACATAGTTATAAACAACACAAATAAAATAGCAGACATGTGCGACAAAATTCAAGTTATCAAAGATAAACTTTATACACCTAAGTTTGAAGACAGCGGTAAAAAACTACATGATTTAGTTTATGAAACAGCATACAAAAAATATGGTGAAAAATTACCAAAAATAGTTGAAGAAAGAATTGAAGCCGAATTAAATCCTATTATTGATTATGGTTTTAGTGTTATTTATTGA", "aa": "MVATADVKYLNLYDKVAYEVLVYAKGIKNSRHYLFNYEQAKNGNIPIPNQDYLTTEEMLEQFAFLKDKQLIEDIVINNTNKIADMCDKIQVIKDKLYTPKFEDSGKKLHDLVYETAYKKYGEKLPKIVEERIEAELNPIIDYGFSVIY", "aa_hexdigest": "10c447d9ea582f3c74b17df5b288700d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17316.68769999999, "isoelectric_point": 5.271327781677246 }, "id": "OAIKOHHJFB_1456", "locus": "GCA000209735_01448" }, { "type": "cds", "sequence": "contig_1", "start": 808416, "stop": 808877, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002285EE07", "PFAM:PF07733.18" ], "nt": "TTGTCAAAAGATGAAAAAATAACAAGTGGATATGACTTACCTAACAAAAATTGCCCTAAATGTAATATTTTATTAGAAGCTGATGGACAAGGAATTCCTTTTGAAACCTTCTTAGGTTTTAAAGCTGATAAAGTTCCTGATATTGACCTTAACTTCTCAGGCGATATTCAAGGTGATATTCACAATGAAGTTAAACGTATTTTTGATAATACTCACACTTTAAAAGCTGGTACCATATCAACAGTTGCTCTTAAAACTTCATATGGACATGTTAAAGCATTTACAGAAGAAGCAATGCGTAATTATTCAGAACCATTTATTGACTTTTTAGCCCATAAAATAGAAAAATTGAAAAGAACTACAGGCCAACACCCAGGTGGTATTCTTATTATTCCAAAAGAATTTGATGTCTATGATTTTACCCCAATAAACTATCCAGCCAATGAACCTGATTCTTCTTGA", "aa": "MSKDEKITSGYDLPNKNCPKCNILLEADGQGIPFETFLGFKADKVPDIDLNFSGDIQGDIHNEVKRIFDNTHTLKAGTISTVALKTSYGHVKAFTEEAMRNYSEPFIDFLAHKIEKLKRTTGQHPGGILIIPKEFDVYDFTPINYPANEPDSS", "aa_hexdigest": "50d8758c418fb2a449357f2399f21c86", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002285EE07", "query_cov": 0.9738562091503268, "subject_cov": 0.8662790697674418, "identity": 0.611, "score": 201.0, "evalue": 1.19e-63, "valid": false, "uniref50_id": "UniRef50_UPI002285EE07" }, "pscc": { "uniref50_id": "UniRef50_UPI002285EE07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002285EE07" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07733.18", "name": "DNA_pol3_alpha", "length": 62, "aa_cov": 0.40522875816993464, "hmm_cov": 1.0, "evalue": 2.529906535454474e-22, "score": 75.78413391113281, "start": 30, "stop": 91 } ], "seq_stats": { "molecular_weight": 17109.088299999985, "isoelectric_point": 5.311001396179199 }, "id": "OAIKOHHJFB_1457", "locus": "GCA000209735_01449" }, { "type": "cds", "sequence": "contig_1", "start": 808965, "stop": 809417, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17657.6" ], "nt": "TTGCTTAAAAAATATACCAATATTAATATTGATGACATTCCAAAAAATGATCCTAAAGTTTTAAGTTTATTTGCAAGTACTAAAGCTATGAATATTAAACCTGAACAAATTAATAATGAAACAACAGGGGCATTAGGTTTGCCTGAATTCGGAACAAGTTTTGTTCGTCAAATGTTAAAAGAAGCTAAACCAAAATCATTTGCTAACCTTATTTCTTTAGCTGGACTTTCTCATGGTGAAAATGTTTGGTTGAACAATGCTCAAGAATTAGTAAGCAAAAAGAATTTTGAAATAACTGATGTTATTTGTTGTCGTGACGACATTATGCAATTTCTTTTAAAGAAAAAAGTTGAACCTTTATTTGCTTTCAATATTATGGAAAAAGTTCGTAAAGGTAAAGGTTTAACTGATGATGAAGTTAAGAGATTAAAAGAATATAAAGTGGCTGACTGA", "aa": "MLKKYTNINIDDIPKNDPKVLSLFASTKAMNIKPEQINNETTGALGLPEFGTSFVRQMLKEAKPKSFANLISLAGLSHGENVWLNNAQELVSKKNFEITDVICCRDDIMQFLLKKKVEPLFAFNIMEKVRKGKGLTDDEVKRLKEYKVAD", "aa_hexdigest": "fbb2e4c24cab980a61775e214946f624", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17657.6", "name": "DNA_pol3_finger", "length": 149, "aa_cov": 0.88, "hmm_cov": 0.8993288590604027, "evalue": 2.598436470270617e-23, "score": 78.26101684570312, "start": 5, "stop": 136 } ], "seq_stats": { "molecular_weight": 17006.64089999999, "isoelectric_point": 9.001349830627444 }, "id": "OAIKOHHJFB_1458", "locus": "GCA000209735_01450" }, { "type": "cds", "sequence": "contig_1", "start": 809580, "stop": 809765, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGATATTAAAGGTGGACATAAAGTTACAGACAGATTGAATCAATTGAATAACATTCCAAAGAATGAAAGAAAAGTTAAAGATGATGACTTAATTCCAGTATTTGAAATAGCTCAAGAACTTTATGCTCGTGGTTTCTATATTGCTAATATTGACTTGAAAAAATCGGCAGCACATGAATGA", "aa": "MIDIKGGHKVTDRLNQLNNIPKNERKVKDDDLIPVFEIAQELYARGFYIANIDLKKSAAHE", "aa_hexdigest": "95c467d4d1a1aa75eca96e0ba2a7235b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7007.9381, "isoelectric_point": 6.723052406311036 }, "id": "OAIKOHHJFB_1459", "locus": "GCA000209735_01451" }, { "type": "cds", "sequence": "contig_1", "start": 809720, "stop": 809947, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAGTTTATCCATTTTTTCAATTAATGTTTTATTAACTGAAGTACGGCGAATAACATCTTCTTTTGAAGTAAAATCTTTAGCATCTCTAGCTTCTACTAAAGATACTGCAGCTGCATCACCTAATCCATCAATTGATTTAAAAGGAGGAATTAAACAACCGTGTTTATTGTCAATAATTCATTCATGTGCTGCCGATTTTTTCAAGTCAATATTAGCAATATAG", "aa": "MLSLSIFSINVLLTEVRRITSSFEVKSLASLASTKDTAAASPNPSIDLKGGIKQPCLLSIIHSCAADFFKSILAI", "aa_hexdigest": "9d82021fc778bcb9c662e20b866a2073", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7952.268399999997, "isoelectric_point": 8.768876838684083 }, "id": "OAIKOHHJFB_1460", "locus": "GCA000209735_01452" }, { "type": "cds", "sequence": "contig_1", "start": 810063, "stop": 810950, "strand": "-", "frame": 3, "gene": null, "product": "ATPase, AAA family", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_F9PSX5" ], "nt": "TTGTTAAATCTTGAAAACGATTTAAAGAAAAGAATAAAAGGGCAAGATGCTGCCATTAGATTAGTTGCTCAAGCTGTATTAAGAGCTAAAGCAAATATTAATGATCCTAATAGGCCTTTAGCAAGTTTCTTATTTACAGGACCTACTGGTGTTGGTAAAACAGAATTAGCAAGAGCTCTTGCATATTCTCTTTTTGATAGTGAAAAACAAATGATTCGTTTAGACATGTCTGAATACATGGAAAAACATAGTGTTTCTAAAATCATTGGTGCTCCTCCAGGATATGTTGGATATGACAGCGGTGGATCATTAACTGAAAAAATTAGAAAGAATCCTTACACAATTCTTTTATTCGATGAAATCGAAAAAGCTGACCGCGATGTTCTTAACATTCTTTTACAAATGATGGATAATGGGGCTATTACAGACTCAAAAGGCCGTGTAATTAACTGTCGTAATTTAATTATAATTATGACAAGTAATTTAGGCTCACAAGAAATACTTAAACATTTAGATCAAATGCCAAGTATTAAAGCTGAATTGCTTAAATTCTTGAGTCCTGAATTTGTAAACAGAATTGATGAAATTGTCAAATTTAATCCATTAACTAAAGATGTAATTAATGAAATTGTTGAACTTGAACTTCAAAAATTAGCTACTAGAATGAAAGATGTTAAAGATGTAAAAATATCATTCACAAGCAAAACTGTTGACTTTGTAGGTTCTTCTTCATATGATGAAAACTTTGGGGCAAGGCCTATTAAAAGATTTATCCAAAATAAAATTGAAAGCTTATTAGCTTACAAGATTATTGATGGCACCATAAGAAGCGGAAAAGCCTATAAAATAGACGTTTTCGCCGGAAACTTTGTTGTTAAAGAAGCCTAA", "aa": "MLNLENDLKKRIKGQDAAIRLVAQAVLRAKANINDPNRPLASFLFTGPTGVGKTELARALAYSLFDSEKQMIRLDMSEYMEKHSVSKIIGAPPGYVGYDSGGSLTEKIRKNPYTILLFDEIEKADRDVLNILLQMMDNGAITDSKGRVINCRNLIIIMTSNLGSQEILKHLDQMPSIKAELLKFLSPEFVNRIDEIVKFNPLTKDVINEIVELELQKLATRMKDVKDVKISFTSKTVDFVGSSSYDENFGARPIKRFIQNKIESLLAYKIIDGTIRSGKAYKIDVFAGNFVVKEA", "aa_hexdigest": "a3df4c8b16e27c37e6fa183f8abd6563", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F4C5962", "query_cov": 1.006779661016949, "subject_cov": 0.8114754098360656, "identity": 0.529, "score": 324.0, "evalue": 4.09e-107, "valid": false, "product": "AAA family ATPase", "uniref50_id": "UniRef50_F9PSX5" }, "pscc": { "uniref50_id": "UniRef50_F9PSX5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_F9PSX5" ], "product": "ATPase, AAA family" }, "genes": [], "id": "OAIKOHHJFB_1461", "locus": "GCA000209735_01453" }, { "type": "cds", "sequence": "contig_1", "start": 810996, "stop": 811214, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCTAATTTAAAAAGTAAATTAGATGACGCAAGACATAGATTAAATATTTACCAAAAAGAAACAGATTATGAAAAAGCTTCAAAAATTTTGTATGATGAAATTCCAAGCATTAAGAAACAAATCGAAGAAACAGAAAAAGAAATAGCAGAATCAGGTAACACAATTGTTAAAGATAGTGTTACAAGTGAAGAAATTGCTAACATTGTTTCGAAATGA", "aa": "MSNLKSKLDDARHRLNIYQKETDYEKASKILYDEIPSIKKQIEETEKEIAESGNTIVKDSVTSEEIANIVSK", "aa_hexdigest": "44b4d03dc0ad878358a320492855b9e7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8257.164299999997, "isoelectric_point": 5.130139732360839 }, "id": "OAIKOHHJFB_1462", "locus": "GCA000209735_01454" }, { "type": "cds", "sequence": "contig_1", "start": 811242, "stop": 812216, "strand": "-", "frame": 3, "gene": null, "product": "ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D648", "UniRef:UniRef90_A0A3P8KMA1" ], "nt": "ATGGATTTTAATTTAGATGACTTATTTGGTTCTAATGACAATAAAAGTAAAAAATCAAGTGATCCATTAAAAATTTATGGAAGAAACTTAACTGATTTAGCTGCTCGGAATGAATTAGATCCTGTTATTAATCGTGATGATGAAATCAGAAGAATGATTCGCATTTTGAGTAGGAAAACAAAAAACAACCCAGTATTAGTCGGCGAACCAGGTGTTGGTAAAACAGCTATTGTTGAAGGATTGGCTAGAAAAATAGTTGAGGGTCAAGTACCAGAAAACTTAAAAGGTAAAGATATTTTTGAACTTGACTTAGCTGCTTTAATAGCTGGCGCTTCATATCAAGGACAATTTGAAAAGAGATTAAAAGACGTACTCAAAAGAATTGAAGAATCTAATGGTGACATTATAGTTTTCATTGATGAAATTCACATGCTTATTGGAACAGGTCGAAATGCTGAAGGTGGTATGGATGCTGCTAACATCATTAAACCTTTAATGGCCAGAGGTAAAATGCATTTAATTGGTGCTACAACTTTTGATGAATATAGAAAATATATAGAAAAAGATGCGGCTCTTGAAAGAAGAATGCAAAGAGTGGATGTATTAGAGCCTTCAATTGAAGATACTATTACAATTCTTCGTGGTATCAAAGACCGTTTTGAAAATTACCACAATGTAAAAATTCAAGATGATGCTTTAGTAGCTGCTGCTCGTCTTTCTTCAAGATATATTTCTGATAGATTTTTACCAGATAAAGCTATTGACTTAGTTGATGAAGCTGCTGCAACTATTAAAACAGAAATAAACTTTCAACCTGAAGAATTAGAAAAAGCTAAACAAAAAATGGCTAGATTGAAAATGGAAAAAATAGCCATTTCTAGTGAAGACAAGAAAAAACATGCAGTTAGACTTAAAGAAATTGATGCCGAAATAAAAGATATTGAAAAAACAATAAAACAATTAGAAGATAAATGA", "aa": "MDFNLDDLFGSNDNKSKKSSDPLKIYGRNLTDLAARNELDPVINRDDEIRRMIRILSRKTKNNPVLVGEPGVGKTAIVEGLARKIVEGQVPENLKGKDIFELDLAALIAGASYQGQFEKRLKDVLKRIEESNGDIIVFIDEIHMLIGTGRNAEGGMDAANIIKPLMARGKMHLIGATTFDEYRKYIEKDAALERRMQRVDVLEPSIEDTITILRGIKDRFENYHNVKIQDDALVAAARLSSRYISDRFLPDKAIDLVDEAAATIKTEINFQPEELEKAKQKMARLKMEKIAISSEDKKKHAVRLKEIDAEIKDIEKTIKQLEDK", "aa_hexdigest": "bee78580c5c23544b9149be44aecdab8", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_UPI002AC75C79", "query_cov": 0.8858024691358025, "subject_cov": 0.9896551724137931, "identity": 0.634, "score": 347.0, "evalue": 1.51e-116, "valid": false, "product": "AAA family ATPase", "uniref50_id": "UniRef50_A0A930RWV4" }, "genes": [], "truncated": "both", "pseudogene": { "start": 810939, "stop": 812288, "observations": { "mutation": [ 810998, 811244, 812240, 812249 ], "stop": [ 810998, 811244, 812240, 812249 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8KMA1", "query_cov": 1.0, "subject_cov": 0.43724696356275305, "identity": 1.0, "score": 611.0, "evalue": 3.4e-214, "gene_start": 1, "gene_end": 324, "reference_start": 25, "reference_end": 348, "reference_sequence": "MIFGTLTLKVLKLWYNWFSIGGEIMDFNLDDLFGSNDNKSKKSSDPLKIYGRNLTDLAARNELDPVINRDDEIRRMIRILSRKTKNNPVLVGEPGVGKTAIVEGLARKIVEGQVPENLKGKDIFELDLAALIAGASYQGQFEKRLKDVLKRIEESNGDIIVFIDEIHMLIGTGRNAEGGMDAANIIKPLMARGKMHLIGATTFDEYRKYIEKDAALERRMQRVDVLEPSIEDTITILRGIKDRFENYHNVKIQDDALVAAARLSSRYISDRFLPDKAIDLVDEAAATIKTEINFQPEELEKAKQKMARLKMEKIAISSEDKKKHAVRLKEIDAEIKDIEKTIKQLEDKWNFEKQRLEELSNLKSKLDDARHRLNIYQKETDYEKASKILYDEIPSIKKQIEETEKEIAESGNTIVKDSVTSEEIANIVSKWTKIPISKLLESDKEKLLNLENDLKKRIKGQDAAIRLVAQAVLRAKANINDPNRPLASFLFTGPTGVGKTELARALAYSLFDSEKQMIRLDMSEYMEKHSVSKIIGAPPGYVGYDSGGSLTEKIRKNPYTILLFDEIEKADRDVLNILLQMMDNGAITDSKGRVINCRNLIIIMTSNLGSQEILKHLDQMPSIKAELLKFLSPEFVNRIDEIVKFNPLTKDVINEIVELELQKLATRMKDVKDVKISFTSKTVDFVGSSSYDENFGARPIKRFIQNKIESLLAYKIIDGTIRSGKAYKIDVFAGNFVVKEA" }, "paralog": false, "identity": 0.9888641425389755, "score": 847.0, "evalue": 2.67e-309, "description": "Internal stop codon at 810998, 811244, 812240, 812249. Nonsense mutation around 810998, 811244, 812240, 812249.", "psc": { "uniref90_id": "UniRef90_A0A3P8KMA1", "product": "ATPase", "uniref50_id": "UniRef50_I5D648", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8KMA1", "UniRef:UniRef50_I5D648" ] }, "pscc": { "uniref50_id": "UniRef50_I5D648", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D648" ], "product": "ATP-dependent Clp protease ATP-binding subunit" } }, "id": "OAIKOHHJFB_1463", "locus": "GCA000209735_01455" }, { "type": "cds", "sequence": "contig_1", "start": 812380, "stop": 812952, "strand": "+", "frame": 1, "gene": null, "product": "Resolvase/invertase-type recombinase catalytic domain-containing protein", "db_xrefs": [ "KEGG:K14060", "RefSeq:WP_013355037.1", "SO:0001217", "UniParc:UPI0001E33078", "UniRef:UniRef100_C4XFQ9", "UniRef:UniRef50_C4XFQ9", "UniRef:UniRef90_C4XFQ9" ], "nt": "ATGATTTATGGTTATACAAGAGTTAGCACAAAAAAACAAATTGATGGATATGGTTTTGAAATACAACAAAATGAAATATTGAATAAATATCCAAACGCAATAATAATTAATGAACAATTTACAGGAACAAAATTAAATAGACCACTATTTAATGATCTATTAACTAAATTAGAAAAAGATGATATTCTAGTAGTTTCTAAATTAGATAGATTTGCTAGAAATACAGTTGAAGGTATTAAAGTAGTTGAAGCTTTATTTAAAAAGAAAGTAGCAATACATATTTTAAATATAGGCTTATTAGAGGACACTCCAATGGGTCGTTTTTTCTTAACAACTATGTTAGCAGTTGCTGAATTAGAAAGAAACATGATAGTAGAAAGAACACAAAATGGTAAAGAGATAGCAAGACTAAGAACAGATTATAAAGAAGGTAGACCAAAAGTTTATACTGAAGATCTTTTAAGTGAAGCAATTTTGCTTTTAGATTCCTTTTCCTATCGCCAAGTTTCACTTTTAACTGGTATTAGTAAATCAACATTAGTTAGAGCTAAAAAAGCACTAAAAAATAGCTAG", "aa": "MIYGYTRVSTKKQIDGYGFEIQQNEILNKYPNAIIINEQFTGTKLNRPLFNDLLTKLEKDDILVVSKLDRFARNTVEGIKVVEALFKKKVAIHILNIGLLEDTPMGRFFLTTMLAVAELERNMIVERTQNGKEIARLRTDYKEGRPKVYTEDLLSEAILLLDSFSYRQVSLLTGISKSTLVRAKKALKNS", "aa_hexdigest": "9b58a564681be0c4a065fead940ccb60", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001E33078", "ncbi_nrp_id": "WP_013355037.1", "uniref100_id": "UniRef100_C4XFQ9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33078", "RefSeq:WP_013355037.1", "UniRef:UniRef100_C4XFQ9" ] }, "ips": { "uniref100_id": "UniRef100_C4XFQ9", "uniref90_id": "UniRef90_C4XFQ9" }, "psc": { "uniref90_id": "UniRef90_C4XFQ9", "product": "Resolvase/invertase-type recombinase catalytic domain-containing protein", "uniref50_id": "UniRef50_C4XFQ9", "kegg_orthology_id": "K14060" }, "pscc": { "uniref50_id": "UniRef50_C4XFQ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFQ9" ], "product": "Resolvase/invertase-type recombinase catalytic domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_1464", "locus": "GCA000209735_01456" }, { "type": "cds", "sequence": "contig_1", "start": 813262, "stop": 813639, "strand": "+", "frame": 1, "gene": null, "product": "Type I restriction modification DNA specificity domain-containing protein", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_C4XFR0", "UniRef:UniRef90_C4XFR0" ], "nt": "TTGAAAAAAGGTACTGTAGTTATTTCTATAACAGGAAATATAAGGGTCAGTTATCTTGCAATTGATTCATGTATAAATCAATCTATAGTAGGAATAGAAGAAAATGAGTTATTGAAAATAGGATATTTATATCCTTTTTTAAAGAATAAAATTGAGTTTTTAATAAGATCTTCTACTGGGAACTGTCAAAAACATATAAATAAAAATTTTATAGAAAATTTAAAAATTGTTTTACCACCAAAAAATGTTTTGGACATATTTAATAATTTAACTCAAAATATATATGCTAAAATAAGTCAAATCAGTTTAATGACTAAGAAATTAATAAAATTCAAAAATAAGCTATTACCTTTATTGATTAATCAACAAATTATTTAA", "aa": "MKKGTVVISITGNIRVSYLAIDSCINQSIVGIEENELLKIGYLYPFLKNKIEFLIRSSTGNCQKHINKNFIENLKIVLPPKNVLDIFNNLTQNIYAKISQISLMTKKLIKFKNKLLPLLINQQII", "aa_hexdigest": "f1bf1e76eae9959ca484347e32675609", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 812962, "stop": 813639, "observations": { "mutation": [ 812995, 813076, 813151, 813169 ], "stop": [ 812995, 813076, 813151, 813169 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFR0", "query_cov": 1.0, "subject_cov": 0.5555555555555556, "identity": 0.992, "score": 237.0, "evalue": 1.09e-77, "gene_start": 1, "gene_end": 125, "reference_start": 101, "reference_end": 225, "reference_sequence": "MQVMGQAIFNRWFLQFEHFKKDNKFKYNEDLNLKIPENWEVKKIAEICKIFLGGTPSTKNREYWNGEINWLNSGEVANFPIIDSEKTINEKGLKNSNTKLLKKGTVVISITGNIRVSYLAIDSCINQSIVGIEENELLKIGYLYPFLKNKIEFLIRSSTGNCQKHINKNFIENLKIVLPPKNVLDIFNNLTQNIYAKISQISLMTKKLIKFKNKLLPLLINQQII" }, "paralog": false, "identity": 0.9777777777777777, "score": 430.0, "evalue": 4.97e-156, "description": "Internal stop codon at 812995, 813076, 813151, 813169. Nonsense mutation around 812995, 813076, 813151, 813169.", "psc": { "uniref90_id": "UniRef90_C4XFR0", "product": "Type I restriction modification DNA specificity domain-containing protein", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_C4XFR0", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFR0", "EC:3.1.21.3", "UniRef:UniRef50_C4XFR0", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFR0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFR0" ], "product": "Type I restriction modification DNA specificity domain-containing protein" } }, "id": "OAIKOHHJFB_1465", "locus": "GCA000209735_01457" }, { "type": "cds", "sequence": "contig_1", "start": 813663, "stop": 814463, "strand": "-", "frame": 3, "gene": null, "product": "Type I restriction modification DNA specificity domain-containing protein", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_C4XFR1", "UniRef:UniRef90_C4XFR1" ], "nt": "ATGTTTCTTTATTATCTTTTAATGTATATGACACCTTATTGGCTTTCATCAACTAATGGATCAATAATGATTCATATTACAAAAGAAACAATGGAAGAAAAAATTGTTGATTTGCCTCCACTAAAAACTCAAAAAAAGATTTCTAAAATATTAGAAAATTTAGACAAGCAGATTGAAAAGAATTTGCATATAGTCAAAAGATTACAAGTTATGGGACAGGCTATTTTTGATATGTTTTTGAATAATGCGAAAGATTATGAAAATATAGAAAGTTTGTGCAAAATAATTTGGGGTCAGTGTCCAAAAGGAAACAACATTTTAAGCGAAAACGTATCAAATAATTTAATGCTTTATGCATCAGGAGCAGGTGATTTAGAGAATAATAAAATATTAATTTCTCCTAAAGCTTTTACTGATAAACCAATTAAAATAATAGATAATAGGACAATTTGTATGTCTATTGCGGGAACTGTTGGTAAAATTGGAATAAGTGATAAAAATATTGCAATTGGGAGAGCTATGGTTGGATTTTATAATGAAAAAAAATTTGGACTAATTTATTTTATTCTAAATAAATATAGTTCATTTTTAAAAAGACAATCAATTGGAGCTATACAAAAAATTATTAACAAAAATCATCTCAATATTGTGAATGTTCCAATATTAACAAACGAAAAAAACAATTTATTAAACGAATTAATAACTAAATGCATGAAATTAGAAAAAAATACTTTAAGTTTGATAAAGCTTAAAGAAAAGTTGATACCTTTATTAATAAATGGCCAAATAGAAATAAATTAG", "aa": "MFLYYLLMYMTPYWLSSTNGSIMIHITKETMEEKIVDLPPLKTQKKISKILENLDKQIEKNLHIVKRLQVMGQAIFDMFLNNAKDYENIESLCKIIWGQCPKGNNILSENVSNNLMLYASGAGDLENNKILISPKAFTDKPIKIIDNRTICMSIAGTVGKIGISDKNIAIGRAMVGFYNEKKFGLIYFILNKYSSFLKRQSIGAIQKIINKNHLNIVNVPILTNEKNNLLNELITKCMKLEKNTLSLIKLKEKLIPLLINGQIEIN", "aa_hexdigest": "2772fdf5fd13c481cc24e4e0aff158a7", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 813663, "stop": 814754, "observations": { "mutation": [ 814487, 814499, 814562, 814628 ], "stop": [ 814487, 814499, 814562, 814628 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFR1", "query_cov": 1.0, "subject_cov": 0.7327823691460055, "identity": 1.0, "score": 508.0, "evalue": 3.92e-180, "gene_start": 1, "gene_end": 266, "reference_start": 98, "reference_end": 363, "reference_sequence": "MMLVKLKDIVTFINGRAYSQPELQDKGKYRIVRVGNFSGKNEWFYSDMELNEDKYCENGDLLYKWACNFGPEIWKSEKTIFHYHIWKIKWDEKRVDKMFLYYLLMYMTPYWLSSTNGSIMIHITKETMEEKIVDLPPLKTQKKISKILENLDKQIEKNLHIVKRLQVMGQAIFDMFLNNAKDYENIESLCKIIWGQCPKGNNILSENVSNNLMLYASGAGDLENNKILISPKAFTDKPIKIIDNRTICMSIAGTVGKIGISDKNIAIGRAMVGFYNEKKFGLIYFILNKYSSFLKRQSIGAIQKIINKNHLNIVNVPILTNEKNNLLNELITKCMKLEKNTLSLIKLKEKLIPLLINGQIEIN" }, "paralog": false, "identity": 0.9862258953168044, "score": 712.0, "evalue": 4.6e-263, "description": "Internal stop codon at 814487, 814499, 814562, 814628. Nonsense mutation around 814487, 814499, 814562, 814628.", "psc": { "uniref90_id": "UniRef90_C4XFR1", "product": "Type I restriction modification DNA specificity domain-containing protein", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_C4XFR1", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFR1", "EC:3.1.21.3", "UniRef:UniRef50_C4XFR1", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFR1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFR1" ], "product": "Type I restriction modification DNA specificity domain-containing protein" } }, "id": "OAIKOHHJFB_1466", "locus": "GCA000209735_01458" }, { "type": "cds", "sequence": "contig_1", "start": 814626, "stop": 814751, "strand": "-", "frame": 3, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF44826", "UniRef:UniRef90_UPI000FF44826" ], "nt": "ATGCTTGTTAAATTGAAAGATATTGTAACTTTCATTAATGGAAGAGCCTATTCTCAACCTGAATTACAAGACAAGGGAAAATATAGAATAGTGCGAGTAGGAAATTTTTCTGGTAAAAATGAATGA", "aa": "MLVKLKDIVTFINGRAYSQPELQDKGKYRIVRVGNFSGKNE", "aa_hexdigest": "7a0be707c383d09657e1b0ce1df1f77d", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 814497, "stop": 814751, "observations": { "mutation": [ 814562, 814628 ], "stop": [ 814562, 814628 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000FF44826", "query_cov": 1.0, "subject_cov": 0.4880952380952381, "identity": 0.976, "score": 83.2, "evalue": 4.73e-20, "gene_start": 1, "gene_end": 41, "reference_start": 1, "reference_end": 41, "reference_sequence": "MLVKLKDIVTFINERAYSQPELQDKGKYRIVRVGNFSGKNEWFYSDMELNEDKYCENGDLLYKWACNFGPEIWKSEKTIFHYHI" }, "paralog": false, "identity": 0.9642857142857143, "score": 170.0, "evalue": 7.84e-57, "description": "Internal stop codon at 814562, 814628. Nonsense mutation around 814562, 814628.", "psc": { "uniref90_id": "UniRef90_UPI000FF44826", "product": "restriction endonuclease subunit S", "uniref50_id": "UniRef50_UPI000FF44826", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000FF44826", "UniRef:UniRef50_UPI000FF44826" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000FF44826", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF44826" ], "product": "restriction endonuclease subunit S" } }, "id": "OAIKOHHJFB_1467", "locus": "GCA000209735_01459" }, { "type": "cds", "sequence": "contig_1", "start": 814762, "stop": 815004, "strand": "-", "frame": 2, "gene": null, "product": "Type I restriction modification DNA specificity domain-containing protein", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_C4XFR2", "UniRef:UniRef90_C4XFR2" ], "nt": "ATGGCAAGATTTTATTTTTATTTTCTTTTACATTTAAACTTAAATAAATTTCATATAGGTTCTTCACAGCCTTTATTAACTCAAGGAATTTTAGGAAACATAAAAATAAATATTCCTAGTTTAAATAAGTGCCAAAAAATATCAAAAATATTAGATAACATAGATAATCAAATTGAGCGAAATAATTCTATGGTTCAAAAGTTACAGTCTTTTGAGCAAGCATTAATTTTCTCAAAAAAGTGA", "aa": "MARFYFYFLLHLNLNKFHIGSSQPLLTQGILGNIKINIPSLNKCQKISKILDNIDNQIERNNSMVQKLQSFEQALIFSKK", "aa_hexdigest": "579c9e55c6c3168ba40eea66978cb81d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 814741, "stop": 815259, "observations": { "mutation": [ 814764, 815130 ], "stop": [ 814764, 815130 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFR2", "query_cov": 1.0, "subject_cov": 0.46511627906976744, "identity": 1.0, "score": 154.0, "evalue": 3.19e-46, "gene_start": 1, "gene_end": 80, "reference_start": 86, "reference_end": 165, "reference_sequence": "MIRINMEYKLKELGIFETGSTLIKKIGNFPAFGGNGIITYVNKWNVDEDAIIIGRVGANCGCVNITNKKSFVTDNALIFKPKEKNMARFYFYFLLHLNLNKFHIGSSQPLLTQGILGNIKINIPSLNKCQKISKILDNIDNQIERNNSMVQKLQSFEQALIFSKKWGANYAC" }, "paralog": false, "identity": 0.9825581395348837, "score": 338.0, "evalue": 1.62e-121, "description": "Internal stop codon at 814764, 815130. Nonsense mutation around 814764, 815130.", "psc": { "uniref90_id": "UniRef90_C4XFR2", "product": "Type I restriction modification DNA specificity domain-containing protein", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_C4XFR2", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFR2", "EC:3.1.21.3", "UniRef:UniRef50_C4XFR2", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFR2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFR2" ], "product": "Type I restriction modification DNA specificity domain-containing protein" } }, "id": "OAIKOHHJFB_1468", "locus": "GCA000209735_01460" }, { "type": "cds", "sequence": "contig_1", "start": 815128, "stop": 815244, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATATAAATTAAAAGAATTAGGTATATTTGAAACTGGAAGCACATTAATCAAAAAAATTGGAAATTTTCCTGCTTTTGGAGGCAATGGCATTATTACATATGTGAATAAATGA", "aa": "MEYKLKELGIFETGSTLIKKIGNFPAFGGNGIITYVNK", "aa_hexdigest": "1b63389fc682220f1b547564c3e65f25", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4192.872100000002, "isoelectric_point": 9.224861717224119 }, "id": "OAIKOHHJFB_1469", "locus": "GCA000209735_01461" }, { "type": "cds", "sequence": "contig_1", "start": 815261, "stop": 815686, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCATCAAATATTTTTGCTAATACTGGAACTAACGTATCCGTTTTATTTATAGATAAAACAAATACTAATGGTGAAGTGTTATTAATGGACGCTTCAAAAATGGGTCATAAAGAAAAAGTTAAAGATCTTCAAAAAACAGTTTTAACTCATGATGAATTAAACAAAATAGTTAATGATTTTGTTAATCATAAAGTTGAAGATGATTTTACAATTTCAGTTACCTATGATCAAATAAAAGAAAGAAACTATTCATTATCAGCTGGTCAATATTTTGAAGTAAAAATTGAATATGTTGATATTACTCAAGAAGAATTTGACAAAATGATTGATGACTTTAATAAAGAATTAGAACAATTTACAAAAGATACAGAAGAATTCCAAAAAGAGATGTCTAAAACATTGAAGGAGTTGAAGCTAAAATAA", "aa": "MPSNIFANTGTNVSVLFIDKTNTNGEVLLMDASKMGHKEKVKDLQKTVLTHDELNKIVNDFVNHKVEDDFTISVTYDQIKERNYSLSAGQYFEVKIEYVDITQEEFDKMIDDFNKELEQFTKDTEEFQKEMSKTLKELKLK", "aa_hexdigest": "2384f6fe1c63022b714c5656a9d56b25", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16470.382100000003, "isoelectric_point": 4.8395788192749025 }, "id": "OAIKOHHJFB_1470", "locus": "GCA000209735_01462" }, { "type": "cds", "sequence": "contig_1", "start": 815705, "stop": 815851, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATTTATCTTTTATTTATTCAACATATTCTTTATTCATTAAAAGAAGGTGGTAAAGCCGCTATAGTTGTACCTACAGGGTTTATTACAGCACAAAGTGGAATTGAAAAAACTATTAGACAATATATTATTGATAGAAAATGA", "aa": "MAIYLLFIQHILYSLKEGGKAAIVVPTGFITAQSGIEKTIRQYIIDRK", "aa_hexdigest": "c27f2ab7268c6135ce0229476ee18b4f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5409.3904999999995, "isoelectric_point": 9.600454521179199 }, "id": "OAIKOHHJFB_1471", "locus": "GCA000209735_01463" }, { "type": "cds", "sequence": "contig_1", "start": 815912, "stop": 816916, "strand": "-", "frame": 1, "gene": null, "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A378PT86" ], "nt": "ATGACTAATTTTACAAAACTTGAAAATAATATAAAATCAATAATCGATGATTTGAAGGGATTGTGTTCAACAAATGGTCTTTCAAATACAGCTGGCGAAGAAATAGTTGTTACTTCTGTCTTTTTATATAAATTTCTAAATGATAAATTCATTTCAAATTTAAAAAAATTTGCAAAAGAATTAAATGTTCCTTTTGAAGAAATAATTAAAAATTCAAACTATATGGATGGATTTTATGATGCTTATCCACAAGATGTTGCATTCAAATATGAAGATACAATTGATTATTTAGTTGGATACATTAATGAAGATGATTTTTATCAAAAATTTGACGATACATTAATTAGAATTTCAGATTATAAACAAAATGAAGCTTTTAATGTTGATACTGCAGAAGGAACTAAAAAGCCACTTTTTGTTCGTCTTAGTACTTTTGTTGAACCTTCAAAAAGAAATAACTTCACAAAAAATGTTTTTTCTTACATTACAAGAGATAGATTTGATTTTGGTGAAGCTTTTAATAATAACTATGATTTCTTTTCAACTATTTTTGAATACCTAATTTCAGATTACAACATTGCAAGTGGTAAATATGCTGAATACTTTACACCTCAAACATTATCAAAAGCTATTGGTGAAATATTAGTTAAAATGTCTCCAATTGAAGATAAAATTTATGAAATATATGACCCAAGTGCAGGTTCTGGATCGCTTGTGTTGCATTTAGCAAATGAACTTGGAGAAGGTAAATTTGGAAATAAGGCTCGTGTTTACACTCAAGATATTTCACAAAAATCATCAAGATTTTTAAGAATAAATATGTTACTTAATGGTTTAAAAGAATCACTAGATAACATAATTGAAGGCGATACCTTACTTACCCCAACACACTATAAAAAAGCAGGAGACGCAACAACAGGTCTTAAACAATTTGATTTTATTACATCTAATCCACCATTCAAAACTGACTTCTCTTCTACTAGAGATAACATTGAATTAATGTGA", "aa": "MTNFTKLENNIKSIIDDLKGLCSTNGLSNTAGEEIVVTSVFLYKFLNDKFISNLKKFAKELNVPFEEIIKNSNYMDGFYDAYPQDVAFKYEDTIDYLVGYINEDDFYQKFDDTLIRISDYKQNEAFNVDTAEGTKKPLFVRLSTFVEPSKRNNFTKNVFSYITRDRFDFGEAFNNNYDFFSTIFEYLISDYNIASGKYAEYFTPQTLSKAIGEILVKMSPIEDKIYEIYDPSAGSGSLVLHLANELGEGKFGNKARVYTQDISQKSSRFLRINMLLNGLKESLDNIIEGDTLLTPTHYKKAGDATTGLKQFDFITSNPPFKTDFSSTRDNIELM", "aa_hexdigest": "871ae26b538784c51a21bc31cda63678", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A378PT86", "query_cov": 0.8083832335329342, "subject_cov": 0.9926470588235294, "identity": 0.6779999999999999, "score": 379.0, "evalue": 1.44e-129, "valid": false, "product": "site-specific DNA-methyltransferase (adenine-specific)", "uniref50_id": "UniRef50_A0A378PT86" }, "pscc": { "uniref50_id": "UniRef50_A0A378PT86", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A378PT86" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "OAIKOHHJFB_1472", "locus": "GCA000209735_01464" }, { "type": "cds", "sequence": "contig_1", "start": 816925, "stop": 818172, "strand": "-", "frame": 2, "gene": null, "product": "type I site-specific deoxyribonuclease", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01153", "SO:0001217", "UniRef:UniRef50_C4XFR4", "UniRef:UniRef90_C4XFR4" ], "nt": "TTGCAAGACAACATAAATAAAAATAATCAAAAAACCTTTAAAGAAAGTAATTATCCAGATATTTTAGTTGTTCACTATATGCTTACAACTGGATATGATGTTAAAAGACTTAAGAAGATGTATTTATTAAGAGGACCTAAAGCTCAAAATCTTTTGCAAACTATTTCACGTGTTAATAGACCCTATCGTAGTCCTAATGGCCAAGAATATCAATATGGATATATAGTTGATTTTATTGATATTGAAAAAGAATACAACAATACTATTGATGCTTATCGTAAAGAAATAGAAGAAGAATACGATTTTGAAGAAGAAGACACTTCCCTTTCTGGTTTAATTATTGATAAAACAGATATCAAAAAAAGATATGATGTTTTAAAAAGTGATCTTGAAAAATTTGTTAATAATTACAATTTAGAAACCTTTGTTGACAAAATTAACAATTTTGACAAAGATACTTTACAAAAGATCAAAAAGATTCTTGTTAATATTAAAAACTGTTCAATTGAATTCAAATTATCACAAGCTGATGAATATTCAAAACTCATTAACGATTATGAAATCAATGTGTTTATTAAAGAAGTACAAAAGAAAATTAATTTTTTAAACTTGCGTTCAGATCCTATTGATACAATTCAATTAATAGATAACAAAGAAGTTGTTCAAATCATTTATGATTTTCTTTTAAAAAATATTACTGTTTTAGATTTAGGTAAATTTGATAAAGAAAATAAAATGGAACTTATTAAACAAAAGTTTATGAAAATGCAAAAAGAAATTAAAGAAAACAAAAATACAAGAGATGTTAAATTTATGAATCTTAATGCATTGATTAAGAAATTACTTGATAAATTAGCTGTTGAGGAATTGACTTACGAAGAAGTAGAAGATGAATTAGATTTAATTTACAATTCAACTAAAAAAATAAATGAAGAAAATAAAGAAATATCTAAAGACTTTGGAAATAGTTTGGCTTTTGTCACAACAATGAATATTGTTTTAGCAGAAACTAAATTAGATCGTTCAATTTTAAAGAACTTTTTATTAATGGTTTATGATGAATTAAAAGGCAACATTTCTGACGAAATCCTCATTAAACAAGGCAAAAATGGATTTATTGACTCAACAAAATCTAAAATAACAACTAGATTAATTAAGAAGAATTTATATAAACAAATTAAAGATAATTACGATTATATTTTGAACGAATTATACTTTAATCTGCTTAATTACAAGGAAACACTTTAA", "aa": "MQDNINKNNQKTFKESNYPDILVVHYMLTTGYDVKRLKKMYLLRGPKAQNLLQTISRVNRPYRSPNGQEYQYGYIVDFIDIEKEYNNTIDAYRKEIEEEYDFEEEDTSLSGLIIDKTDIKKRYDVLKSDLEKFVNNYNLETFVDKINNFDKDTLQKIKKILVNIKNCSIEFKLSQADEYSKLINDYEINVFIKEVQKKINFLNLRSDPIDTIQLIDNKEVVQIIYDFLLKNITVLDLGKFDKENKMELIKQKFMKMQKEIKENKNTRDVKFMNLNALIKKLLDKLAVEELTYEEVEDELDLIYNSTKKINEENKEISKDFGNSLAFVTTMNIVLAETKLDRSILKNFLLMVYDELKGNISDEILIKQGKNGFIDSTKSKITTRLIKKNLYKQIKDNYDYILNELYFNLLNYKETL", "aa_hexdigest": "8b530b40119e903f3b5f7ad67f09757f", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 816925, "stop": 818472, "observations": { "mutation": [ 818232 ], "stop": [ 818232 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFR4", "query_cov": 1.0, "subject_cov": 0.4021317829457364, "identity": 0.998, "score": 789.0, "evalue": 2.5e-278, "gene_start": 1, "gene_end": 415, "reference_start": 618, "reference_end": 1032, "reference_sequence": "MMEFNEDTRVKLPATIHFLKLGYNYQTVRSPEINRDNNIFEERFHRAIEKINKKKFAKEEINELISDISRKIKNKDLGKEFYNRLISTDYPIKLIDFENIENNDFAIVNELTFRSNANSNYDSFRPDITILVNGMPLAFLEVKVKNNKNGIQAEFIRMWEDRLKVDEYRKFFNMLQIVSFTNNMDYEEFNDAEASLLKQGSFYTTPNGKDNTTFSAFREDEPNYHANYSFEEIDNDKIKEILIDNNIKTSHVDSDWFQTNLSDTTPCNRFITSIFDKERFLYFIKYGMMYLSQIKEIKNETLNKVEQLPINQKHIMRYPQFFATRAIIKRFKRTNKNGIIWHTQGSGKTALSAFATKVIKDYWSKKKINARFFFIVDRLDLMRQASAEFANRGFNVVNVDSKEAFAKELNETIDRNNNGIGTICVVNIHKFMDSSKMPEAKNDYNIRTQRIFFIDEAHRSYTMHGEFYKNLMTCDRDGIYIAMTGTPLLTKRERSNLKFGDYIHKYFYDKSIADGYTLRIKKEQIKTEARATIIRNLNIEIEKLESRIIYESDDYVKSVSQYIEKDFIDFRFICRNDLTIGGMIVCRSNDQAKKIHEWFKKNSKLTTGLVLSDTDNPLQDNINKNNQKTFKESNYPDILVVHYMLTTGYDVKRLKKMYLLRGPKAQNLLQTISRVNRPYRSPNGQEYQYGYIVDFIDIEKEYNNTIDAYRKEIEEEYDFEEEDTSLSGLIIDKTDIKKRYDVLKSDLEKFVNNYNLETFVDKINNFDKDTLQKIKKILVNIKNCSIEFKLSQADEYSKLINDYEINVFIKEVQKKINFLNLRSDPIDTIQLIDNKEVVQIIYDFLLKNITVLDLGKFDKENKMELIKQKFMKMQKEIKENKNTRDVKFMNLNALIKKLLDKLAVEELTYEEVEDELDLIYNSTKKINEENKEISKDFGNSLAFVTTMNIVLAETKLDRSILKNFLLMVYDELKGNISDEILIKQGKNGFIDSTKSKITTRLIKKNLYKQIKDNYDYILNELYFNLLNYKETL" }, "paralog": false, "identity": 0.9980582524271845, "score": 1008.0, "evalue": 0.0, "description": "Internal stop codon at 818232. Nonsense mutation around 818232.", "psc": { "uniref90_id": "UniRef90_C4XFR4", "product": "type I site-specific deoxyribonuclease", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_C4XFR4", "kegg_orthology_id": "K01153", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFR4", "EC:3.1.21.3", "UniRef:UniRef50_C4XFR4", "KEGG:K01153" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFR4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFR4" ], "product": "type I site-specific deoxyribonuclease" } }, "id": "OAIKOHHJFB_1473", "locus": "GCA000209735_01465" }, { "type": "cds", "sequence": "contig_1", "start": 818230, "stop": 818880, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGACAAGCTTCTGCAGAATTTGCCAATAGAGGTTTCAATGTTGTTAATGTTGATTCAAAAGAAGCATTCGCTAAAGAATTAAATGAGACAATCGATAGAAATAATAATGGTATTGGCACAATTTGTGTTGTAAACATTCATAAATTTATGGATAGTTCAAAAATGCCAGAAGCTAAAAATGATTACAACATAAGAACTCAAAGAATTTTCTTTATTGATGAAGCTCACAGAAGTTATACAATGCATGGTGAATTTTACAAAAACCTTATGACTTGTGATAGAGATGGAATTTACATTGCAATGACTGGTACACCTTTATTAACTAAAAGAGAAAGATCAAATTTAAAATTTGGAGACTACATTCATAAATACTTTTATGATAAATCAATAGCTGATGGTTATACTTTGAGAATTAAAAAAGAACAAATTAAAACTGAAGCTAGAGCTACAATTATTAGAAACTTAAATATTGAAATTGAAAAATTAGAATCAAGAATTATTTATGAATCAGATGATTATGTTAAAAGCGTTAGCCAATATATAGAAAAAGACTTTATTGATTTTAGATTTATTTGCAGAAATGATTTAACAATTGGTGGAATGATTGTCTGTCGTTCAAATGACCAAGCGAAAAAAATTCATGAATGA", "aa": "MRQASAEFANRGFNVVNVDSKEAFAKELNETIDRNNNGIGTICVVNIHKFMDSSKMPEAKNDYNIRTQRIFFIDEAHRSYTMHGEFYKNLMTCDRDGIYIAMTGTPLLTKRERSNLKFGDYIHKYFYDKSIADGYTLRIKKEQIKTEARATIIRNLNIEIEKLESRIIYESDDYVKSVSQYIEKDFIDFRFICRNDLTIGGMIVCRSNDQAKKIHE", "aa_hexdigest": "dc1ee2318cc28d86bb1673fd40c0666c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25291.487800000028, "isoelectric_point": 8.23810863494873 }, "id": "OAIKOHHJFB_1474", "locus": "GCA000209735_01466" }, { "type": "cds", "sequence": "contig_1", "start": 819001, "stop": 819162, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGTATCTTAGCCAAATAAAAGAAATTAAAAATGAAACTTTAAATAAAGTTGAACAATTGCCAATTAATCAAAAACACATTATGAGATATCCTCAATTTTTCGCTACAAGAGCAATTATCAAAAGATTCAAACGAACTAATAAAAATGGAATTATTTGA", "aa": "MMYLSQIKEIKNETLNKVEQLPINQKHIMRYPQFFATRAIIKRFKRTNKNGII", "aa_hexdigest": "5b21485bac4e6ec319f5f9f95330c7c2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6436.6222, "isoelectric_point": 10.509910011291506 }, "id": "OAIKOHHJFB_1475", "locus": "GCA000209735_01467" }, { "type": "cds", "sequence": "contig_1", "start": 819256, "stop": 819504, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCAAATTGTTTCATTTACAAACAATATGGATTATGAAGAATTTAATGATGCTGAGGCTTCACTACTAAAACAAGGCTCTTTCTATACAACACCAAATGGAAAAGACAATACTACTTTTTCAGCTTTTAGAGAAGATGAACCTAACTACCATGCTAACTACTCTTTTGAGGAAATAGACAATGACAAAATAAAAGAAATTTTAATTGATAATAATATTAAAACTTCACATGTTGATAGTGATTGA", "aa": "MLQIVSFTNNMDYEEFNDAEASLLKQGSFYTTPNGKDNTTFSAFREDEPNYHANYSFEEIDNDKIKEILIDNNIKTSHVDSD", "aa_hexdigest": "ef0950a75bc7fd9a519371e59a491703", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9492.098799999992, "isoelectric_point": 4.251465797424316 }, "id": "OAIKOHHJFB_1476", "locus": "GCA000209735_01468" }, { "type": "cds", "sequence": "contig_1", "start": 819547, "stop": 820020, "strand": "-", "frame": 2, "gene": null, "product": "type I site-specific deoxyribonuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_R5XCY4" ], "nt": "ATGGAATTTAATGAAGATACAAGAGTAAAATTACCAGCAACGATTCACTTTTTAAAACTTGGTTACAATTATCAAACTGTTAGAAGCCCAGAAATTAATAGAGACAATAACATTTTTGAAGAAAGATTTCATAGAGCAATTGAAAAAATAAATAAGAAAAAATTTGCTAAAGAAGAAATAAATGAATTGATCTCTGATATAAGCAGAAAAATCAAAAATAAGGATTTAGGAAAAGAATTTTATAATCGATTAATTTCGACCGATTACCCTATAAAACTGATAGATTTTGAAAATATTGAGAACAATGATTTTGCAATTGTTAATGAATTAACTTTTAGAAGTAACGCTAATTCTAATTATGATTCATTTAGACCAGATATTACAATTTTAGTTAATGGTATGCCATTAGCATTTTTAGAAGTAAAAGTTAAAAACAACAAAAATGGTATTCAAGCAGAATTTATTAGAATGTGA", "aa": "MEFNEDTRVKLPATIHFLKLGYNYQTVRSPEINRDNNIFEERFHRAIEKINKKKFAKEEINELISDISRKIKNKDLGKEFYNRLISTDYPIKLIDFENIENNDFAIVNELTFRSNANSNYDSFRPDITILVNGMPLAFLEVKVKNNKNGIQAEFIRM", "aa_hexdigest": "5a44f6c11b8f9e40968808b5677e8983", "start_type": "ATG", "rbs_motif": "GGAGG", "psc": { "uniref90_id": "UniRef90_R5XCY4", "query_cov": 0.9872611464968153, "subject_cov": 0.9935897435897436, "identity": 0.568, "score": 167.0, "evalue": 1.4e-50, "valid": false, "product": "type I site-specific deoxyribonuclease", "uniref50_id": "UniRef50_R5XCY4" }, "pscc": { "uniref50_id": "UniRef50_R5XCY4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_R5XCY4" ], "product": "type I site-specific deoxyribonuclease" }, "genes": [], "id": "OAIKOHHJFB_1477", "locus": "GCA000209735_01469" }, { "type": "cds", "sequence": "contig_1", "start": 820020, "stop": 820214, "strand": "-", "frame": 3, "gene": null, "product": "HTH cro/C1-type domain-containing protein", "db_xrefs": [ "RefSeq:WP_013355031.1", "SO:0001217", "UniParc:UPI0001E3305F", "UniRef:UniRef100_C4XFR5", "UniRef:UniRef50_C4XFR5", "UniRef:UniRef90_C4XFR5" ], "nt": "ATGGAATTTAAAGACAAAATTTTACGTGCTAGAGCTCTTTTAAACTTAACTCAAGAAGAATTAGCAAAACAACTTGGTGTTTCTTTTAACACCATAAATAGATGGGAAAATGGAAAAGTTAAGCCAACAAAAGTTGCTAAATTGAGATTTGATGAATATTGTAGAAAAAATGGTGTTTTGTTGGAGGTTAGATAA", "aa": "MEFKDKILRARALLNLTQEELAKQLGVSFNTINRWENGKVKPTKVAKLRFDEYCRKNGVLLEVR", "aa_hexdigest": "22909f2efd67cb793c157f1fa02e3864", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI0001E3305F", "ncbi_nrp_id": "WP_013355031.1", "uniref100_id": "UniRef100_C4XFR5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3305F", "RefSeq:WP_013355031.1", "UniRef:UniRef100_C4XFR5" ] }, "ips": { "uniref100_id": "UniRef100_C4XFR5", "uniref90_id": "UniRef90_C4XFR5" }, "psc": { "uniref90_id": "UniRef90_C4XFR5", "product": "HTH cro/C1-type domain-containing protein", "uniref50_id": "UniRef50_C4XFR5" }, "pscc": { "uniref50_id": "UniRef50_C4XFR5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFR5" ], "product": "HTH cro/C1-type domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_1478", "locus": "GCA000209735_01470" }, { "type": "cds", "sequence": "contig_1", "start": 820322, "stop": 820633, "strand": "+", "frame": 2, "gene": null, "product": "tRNA (Guanine-N(7)-)-methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XDE4", "UniRef:UniRef90_A0AB32XDE4" ], "nt": "ATGAGATTAAGAAATGACAAAGATGCACCAAAAAAGCTTGAAGAATCTAATTTATTGATTACTAAAAGTCAATATCCTATTAGCTTAAAAAATACTGATGTAATTGAAATTGGCATGGGTAAAGGCGAAATGATAACTCAATTAGCTGCTCTAAATCCAAATATAAATTTTTATGGTTTAGAAAAGTTTGCTACTGTAGCTGCTAAAAGCGTAAGAAGAGCTAAAGAATATAAGCTTAATAACTTTAAAATTATTTTAGAAGATGCTAAAGAATTAAAAAGCATTTTTAAAGGAACTTGTGGCACAATTTGA", "aa": "MRLRNDKDAPKKLEESNLLITKSQYPISLKNTDVIEIGMGKGEMITQLAALNPNINFYGLEKFATVAAKSVRRAKEYKLNNFKIILEDAKELKSIFKGTCGTI", "aa_hexdigest": "a859b06ff65da50b67809ff42c1d2200", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 820322, "stop": 820742, "observations": { "insertion": [ 820703 ], "mutation": [ 820631, 820652 ], "stop": [ 820631, 820652 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0AB32XDE4", "query_cov": 1.0, "subject_cov": 0.7410071942446043, "identity": 1.0, "score": 199.0, "evalue": 3.14e-64, "gene_start": 1, "gene_end": 103, "reference_start": 1, "reference_end": 103, "reference_sequence": "MRLRNDKDAPKKLEESNLLITKSQYPISLKNTDVIEIGMGKGEMITQLAALNPNINFYGLEKFATVAAKSVRRAKEYKLNNFKIILEDAKELKSIFKGTCGTIWLTFSDPWPKARHFKRRLTYKDFLKNINLLWIKILF" }, "paralog": false, "identity": 0.9856115107913669, "score": 264.0, "evalue": 2.67e-92, "description": "Internal stop codon at 820631, 820652. Frameshift due to insertion around 820703. Nonsense mutation around 820631, 820652.", "psc": { "uniref90_id": "UniRef90_A0AB32XDE4", "product": "tRNA (Guanine-N(7)-)-methyltransferase", "uniref50_id": "UniRef50_A0AB32XDE4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0AB32XDE4", "UniRef:UniRef50_A0AB32XDE4" ] }, "pscc": { "uniref50_id": "UniRef50_A0AB32XDE4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XDE4" ], "product": "tRNA (Guanine-N(7)-)-methyltransferase" } }, "id": "OAIKOHHJFB_1479", "locus": "GCA000209735_01471" }, { "type": "cds", "sequence": "contig_1", "start": 820912, "stop": 821100, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTAAATTTTTTAGAATATAAACCATCAGCTGATCTAAAATCAGGAATTCCTGAAGCTGTTGAAACACCAGCTCCACTAAAGAAAACAATATGTTTAGATTCTTTAATTCAATTTTTAAATTTGTCTATTTTTTCATTAAAATTTTGCACCATATACCTCTATTTAATTTTAGCAAAAATATAA", "aa": "MFLNFLEYKPSADLKSGIPEAVETPAPLKKTICLDSLIQFLNLSIFSLKFCTIYLYLILAKI", "aa_hexdigest": "c841bf6945028a5052c8f73c8ce29cab", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7041.417999999996, "isoelectric_point": 7.743994331359863 }, "id": "OAIKOHHJFB_1480", "locus": "GCA000209735_01472" }, { "type": "cds", "sequence": "contig_1", "start": 821276, "stop": 821698, "strand": "+", "frame": 2, "gene": null, "product": "NAD-dependent protein deacylase", "db_xrefs": [ "EC:2.3.1.286", "GO:0034979", "GO:0051287", "GO:0070403", "SO:0001217", "UniRef:UniRef50_C4XFR7", "UniRef:UniRef90_C4XFR7" ], "nt": "TTGGATATTAAAGCTGGTAATAAATATGTAATTGAATTACATGGAAATATTAATCGTAATTATTGTTTGCTCTGTGGTAAGTTTTATGATTTAGCTCAATTAATTCATCAAAAAGATAAAGATGGAATTCCTACTTGTAAATGTGGGGGAGTAATAAATCCAGATGTAGTTTTATATGAAGATCCACTAATGGAAGATTCAACTAATGATGCAGCTGAAGCCATTTCAAATAGTGATTTATTAATTATTGCAGGTACTTCATTAAGTGTTTATCCTGCAGCTTCATATATTCATTTTTATCAGGGTAAAAGAATAGTAATTCTAAATAAAGATACTTCACGTTATGAAAATAGTAACAATGAAAATATCTTGTTGTTTAATGAAAATATGAAGGATGTTTTTGAAAATTTAGCTAATTCATAG", "aa": "MDIKAGNKYVIELHGNINRNYCLLCGKFYDLAQLIHQKDKDGIPTCKCGGVINPDVVLYEDPLMEDSTNDAAEAISNSDLLIIAGTSLSVYPAASYIHFYQGKRIVILNKDTSRYENSNNENILLFNENMKDVFENLANS", "aa_hexdigest": "92cf2e5d29258527de8c46031b4582df", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 820976, "stop": 821698, "observations": { "mutation": [ 820985, 821132, 821228 ], "stop": [ 820985, 821132, 821228 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFR7", "query_cov": 1.0, "subject_cov": 0.5577689243027888, "identity": 0.993, "score": 281.0, "evalue": 1.52e-94, "gene_start": 1, "gene_end": 140, "reference_start": 112, "reference_end": 251, "reference_sequence": "MVQNFNEKIDKFKNWIKESKHIVFFSGAGVSTASGIPDFRSADGLYSKKFKNMNPESILSRSFWRKNKKDFYEYYFSNIAFDNIKPNIIHETVASWCNKNKCHVITQNIDNLDIKAGNKYVIELHGNINRNYCLLCGKFYDLAQLIHQKDKDGIPTCKCGGVINPDVVLYEDPLMEDSTNDAAEAISNSDLLIIAGTSLSVYPAASYIHFYQGKRIVILNKDTSRYENSNNENILLFNENMKDVFENLANS" }, "paralog": false, "identity": 0.9875, "score": 480.0, "evalue": 2.57e-175, "description": "Internal stop codon at 820985, 821132, 821228. Nonsense mutation around 820985, 821132, 821228.", "psc": { "uniref90_id": "UniRef90_C4XFR7", "product": "NAD-dependent protein deacylase", "ec_ids": [ "2.3.1.286" ], "uniref50_id": "UniRef50_C4XFR7", "go_ids": [ "GO:0034979", "GO:0051287", "GO:0070403" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFR7", "EC:2.3.1.286", "UniRef:UniRef50_C4XFR7", "GO:0034979", "GO:0051287", "GO:0070403" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFR7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFR7" ], "product": "protein acetyllysine N-acetyltransferase" } }, "id": "OAIKOHHJFB_1481", "locus": "GCA000209735_01473" }, { "type": "cds", "sequence": "contig_1", "start": 821733, "stop": 822020, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013355028.1", "SO:0001217", "UniParc:UPI0001E3305D", "UniRef:UniRef100_C4XFR8", "UniRef:UniRef50_C4XFR8", "UniRef:UniRef90_C4XFR8" ], "nt": "ATGAATAAAGATGACAAAAAAGAAAACAGCTTAGAGATTACAACTCTATACGATGCTGAAAAATTTAGAATTATTAAGAATGAAGATTTAAACAATTTAACTAATGAACAAAGAGAAAAATTAGTTAAAGATGCAATTGATAATGCTAAAAAAAATGAAGCCAATATAATTGCAAATAGGAAGAAAAGATATTTGTCTTCTTTAGCTGTAATTTTAGTAGCTGGAATAATCATTGCAATTACAGTAATTTTGATACTTGCACTAATTATTTACTTACATAAAAAATAA", "aa": "MNKDDKKENSLEITTLYDAEKFRIIKNEDLNNLTNEQREKLVKDAIDNAKKNEANIIANRKKRYLSSLAVILVAGIIIAITVILILALIIYLHKK", "aa_hexdigest": "b47f11197ed18bc1e6b5968d894f0d86", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E3305D", "ncbi_nrp_id": "WP_013355028.1", "uniref100_id": "UniRef100_C4XFR8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3305D", "RefSeq:WP_013355028.1", "UniRef:UniRef100_C4XFR8" ] }, "ips": { "uniref100_id": "UniRef100_C4XFR8", "uniref90_id": "UniRef90_C4XFR8" }, "psc": { "uniref90_id": "UniRef90_C4XFR8", "uniref50_id": "UniRef50_C4XFR8" }, "pscc": { "uniref50_id": "UniRef50_C4XFR8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFR8" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10891.683399999989, "isoelectric_point": 9.362372779846194 }, "id": "OAIKOHHJFB_1482", "locus": "GCA000209735_01474" }, { "type": "cds", "sequence": "contig_1", "start": 822059, "stop": 822280, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACCTTGTTTCAAACGCTTGCAATTATTGCTATTTTAATTATTGCTATAAGCTTTGAATATCCTATTGAATTGGTTGAAAAATATGGCCTAACTTTAGTTCAAAGTGATGCTTGTATTTATGCAATTAACACTCTTACAATTGTTGCATTATTCTTCAGTATTTACTCAGGAATATTGTATGTTAAAAAAATAATCCCTTTCCTAGAAATGGTATAA", "aa": "MKTLFQTLAIIAILIIAISFEYPIELVEKYGLTLVQSDACIYAINTLTIVALFFSIYSGILYVKKIIPFLEMV", "aa_hexdigest": "1da5b8764598b52dae3e7412251de1b6", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8249.945299999998, "isoelectric_point": 5.002422523498535 }, "id": "OAIKOHHJFB_1483", "locus": "GCA000209735_01475" }, { "type": "cds", "sequence": "contig_1", "start": 822455, "stop": 822700, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAGAAGATGAATCTACCAAATAAACTAACAATACTTAGATTGATTTTATTCTTACCTCTTTTAGCTCTTTTTATTACTTATAAGATGATCCAAAATTACATGGATAATTACTATAAAATAGCTGGCCGTTCAATATTATCTGGAATTTTACTTATCTTTTTAGGAGCAATGATTACTGACTATTTTGATGGCAAAATTGCTAGAAAATACAATATGATTACAAGCTTTGGAAAGCTGTGA", "aa": "MTKKMNLPNKLTILRLILFLPLLALFITYKMIQNYMDNYYKIAGRSILSGILLIFLGAMITDYFDGKIARKYNMITSFGKL", "aa_hexdigest": "698cabfcd0d8447851f1d1cf11f03082", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9417.429299999993, "isoelectric_point": 9.99648380279541 }, "id": "OAIKOHHJFB_1484", "locus": "GCA000209735_01476" }, { "type": "cds", "sequence": "contig_1", "start": 822834, "stop": 822980, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAACATAATTAATACAGCTCGTGGTTTCTTAGAAAAATATCAAGAAGATGCTTTATTTAAAGAATGTATTTTGAATAATGCTGATGCTAGGGATGAAATTTTTGCTTTTGGTTTTTTAAGAGATACAAGAAGCAATTCGTGA", "aa": "MSNIINTARGFLEKYQEDALFKECILNNADARDEIFAFGFLRDTRSNS", "aa_hexdigest": "a403fecf85cf8ba49e1f15ef2b3a0434", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5546.124199999998, "isoelectric_point": 4.7658586502075195 }, "id": "OAIKOHHJFB_1485", "locus": "GCA000209735_01477" }, { "type": "cds", "sequence": "contig_1", "start": 823309, "stop": 823434, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAGAGAAACTAACTTAATAAAAAATAAGAAAATAAATGACATTTTTGAAGCAATTGGAATAATTGTTACAGTTGATAACGATTTAAAAACAAACAACAAAAAACATAGAATTACATTATGA", "aa": "MERETNLIKNKKINDIFEAIGIIVTVDNDLKTNNKKHRITL", "aa_hexdigest": "de5678d6c1aca33bd754db253ab59e4d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4794.5322, "isoelectric_point": 9.301836967468262 }, "id": "OAIKOHHJFB_1486", "locus": "GCA000209735_01478" }, { "type": "cds", "sequence": "contig_1", "start": 823663, "stop": 823932, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTACAAGCGGCAATATTATTTTGATTCTTACATTTTTGCCTTCACTTTATGTGATTAATAATTTCTTGCCTGAAGCTAATCAAAGTAATGCATTAAAAGCTTTTAACGGCTTTAAGTTTTTAAGAATTTTAAGATTAATTATGCTTTTAAATGTTTTTGTAGTTTTTAAAAATATTTCAGAATCTGTATCAAATGAAAAAGTTTTGTTTTTCAATATCTTCTTATTTATAGGTATTTTGATCGTTTTATTTGCTCTTACAGTTTGA", "aa": "MITSGNIILILTFLPSLYVINNFLPEANQSNALKAFNGFKFLRILRLIMLLNVFVVFKNISESVSNEKVLFFNIFLFIGILIVLFALTV", "aa_hexdigest": "155106bb445b572ee9f6aef75110c8be", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10128.15609999999, "isoelectric_point": 9.819711494445798 }, "id": "OAIKOHHJFB_1487", "locus": "GCA000209735_01479" }, { "type": "cds", "sequence": "contig_1", "start": 824140, "stop": 824436, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGTGCCCTTAATTTCTATTGTTGGTATTGCAATTATTGCTATACCTGGTGGTATGGTAGCAGCAAGTGTTTTAACTAGTTTTCAAAAGAAAAAAGATAATAAAGAAAAAGCTAATGAGCTTGCTGAAATAATTGATGCAGAAATTGAAAGAAGAGTTAAAGAAAGAATTAAAGAAGAAGAAAAAGAAATGAAACACGATTTATTACATCGTTTTGATGAAACAATTCAAAAAGAAAAAGAATTAAAACACACAAACGAACACGCATCTCATGATCATAAAGTTCATAAATAA", "aa": "MIVPLISIVGIAIIAIPGGMVAASVLTSFQKKKDNKEKANELAEIIDAEIERRVKERIKEEEKEMKHDLLHRFDETIQKEKELKHTNEHASHDHKVHK", "aa_hexdigest": "dd89cce1cac6652bb2336aaeff4ab3a8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11283.931399999989, "isoelectric_point": 6.656209754943849 }, "id": "OAIKOHHJFB_1488", "locus": "GCA000209735_01480" }, { "type": "tRNA", "sequence": "contig_1", "start": 824477, "stop": 824566, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(cga)", "amino_acid": "Ser", "anti_codon": "cga", "score": 68.1, "nt": "CGGTAGTTGCTCGAGAGGCTGAAGAGGTTGGTCTCGAAAACCAATAATGGTGAAAGTCATTCAAGGGTTCAAATCCCTTACTACCGGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 824488, 824490 ], "id": "OAIKOHHJFB_17", "locus": "GCA000209735_01481" }, { "type": "cds", "sequence": "contig_1", "start": 824602, "stop": 824994, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAAAAATAGTGTTCTAGAAATAATTCAAAATAAAGCAGAATTTGAAAAAAAAATAAGTACATTAATTTCCCAATATGTCCTTAAATTCTTTATAAACAACAAAAAACCAGGTTATGAAGAATTAATAAAAGAAGTTAAAGTTACTATTGATCCAAATAATGATTATGACGCCTTAAAATATGGCGTCGGTAAGTTACCAATTAGAATAGATTTCATTAATCATAAAAATGAAGATATGATTAAAAACATAAGTGAAAGAAAATTCTTATTAGGCGGTTTTGTTGGTTATGATTTCAGTGATATTGAAGCAAAATTAAAAACATTAACTGATGCTGATTTTGAGAGACCTCTTAAGGATAAAACTCCACCTTATTTAATCAAAAACTAA", "aa": "MNKNSVLEIIQNKAEFEKKISTLISQYVLKFFINNKKPGYEELIKEVKVTIDPNNDYDALKYGVGKLPIRIDFINHKNEDMIKNISERKFLLGGFVGYDFSDIEAKLKTLTDADFERPLKDKTPPYLIKN", "aa_hexdigest": "61d09685d3a716907af0f5a65c5049bf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15118.308199999983, "isoelectric_point": 8.630850028991702 }, "id": "OAIKOHHJFB_1489", "locus": "GCA000209735_01482" }, { "type": "cds", "sequence": "contig_1", "start": 825007, "stop": 825648, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTTCAAGATTCTAATCCTAGATATCATGCATTTCAAAAATATGGAAATTTTGAAATCGATTCTTCATTAGAAACAAATGAAAAAAGCAAATCTTTATTTAATGAAATGTTTAAGGAACCAACACTTTCATTTGAAAAAAATATGTTCAATTTTAATGATTTTGATTCATTAATGCACCCTTCAAAACCTTATTTAGGTTTTAATCTTAATTCTTTTATGCAATTCTATAACGCTTATAAAGAAAAGGTCAAAATTAATGTTCCTGATGAAAATTATGAAGCAAAAATAAAAGAAATAAAATTTGACAAAATATTAAATAATTCACATTCAATAGGCAAGGCTATAGTTGAAATTAAAAACAAAAAAACTAATAGAGTACAAGAAGCTAATTGGTTTACTATTGACTTTACATATGCACATAAACATATATTTGATGGCTTTTATATTCAAAATAAATTAGATTGTGATCCTTTAAAAAATGATGAATTCTTCAGCTATTATATAAATAAAGGAATTGGAACAAATAATGAATTAAAATTACCTGAAGGCATTAATGCAGAAGAATTTTATAACACTAATTTAATAGATATTGTTAACTTTATTATTTATCAAAACAGAGATTCATTTAACATTTGA", "aa": "MDFQDSNPRYHAFQKYGNFEIDSSLETNEKSKSLFNEMFKEPTLSFEKNMFNFNDFDSLMHPSKPYLGFNLNSFMQFYNAYKEKVKINVPDENYEAKIKEIKFDKILNNSHSIGKAIVEIKNKKTNRVQEANWFTIDFTYAHKHIFDGFYIQNKLDCDPLKNDEFFSYYINKGIGTNNELKLPEGINAEEFYNTNLIDIVNFIIYQNRDSFNI", "aa_hexdigest": "a3ecff2df08ff550db9451bd6511186c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25323.087500000023, "isoelectric_point": 5.438718605041504 }, "id": "OAIKOHHJFB_1490", "locus": "GCA000209735_01483" }, { "type": "cds", "sequence": "contig_1", "start": 825931, "stop": 826101, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTTTTGAAAATGATAATTCAAATTTTAAAAATTTAGGCTATGTTAATTATGTTAAAAGATTGTTTAAATCAAAATACAACAATTTAGCAAATGCTTCTAAAAATTTACCTGATTTACAAATGATTTTACAAGTTGCAATTTCAGGAAGTAATAGATTTTCAATTTGA", "aa": "MFFENDNSNFKNLGYVNYVKRLFKSKYNNLANASKNLPDLQMILQVAISGSNRFSI", "aa_hexdigest": "062c32007a20141a52ceb6b1ffa0ed39", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6478.3287, "isoelectric_point": 9.775808525085449 }, "id": "OAIKOHHJFB_1491", "locus": "GCA000209735_01484" }, { "type": "cds", "sequence": "contig_1", "start": 826165, "stop": 826566, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTTATGAGTATGACAAACATTAGCAGAAAGAAGAAGATGAAAAAATATTTTTTGAATAGATTTATTTTAGGTAGTGCTTTATCTTTTGCTCCAATAATATCTGCTTCTTGTGTTCAAAAACAAGAAGTTCCTTTTGAAGACAAATTAATTGAATCAAAATATGAAAATAGTTCAATAATTGGCTTAAAGGACAAAGATTTACTTAAATTAAATTTAAATCAAATAAAGTATTTTATCGAGCAAACTAATAAATTAAGATTAAAAAATAATTTTGAACTAAAACAAGTTGTAAAAGAAAATGATAATCTATTTTTGTCAATAAATAATAAAAAATATTCATTAAAAAGTTTTTTAAAATCAGTTAATGATAATTGGGATATATTATCAAAAAATTGA", "aa": "MVFMSMTNISRKKKMKKYFLNRFILGSALSFAPIISASCVQKQEVPFEDKLIESKYENSSIIGLKDKDLLKLNLNQIKYFIEQTNKLRLKNNFELKQVVKENDNLFLSINNKKYSLKSFLKSVNDNWDILSKN", "aa_hexdigest": "8205a24575aa2e93087e14736b5e351d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15622.147399999987, "isoelectric_point": 9.760078239440915 }, "id": "OAIKOHHJFB_1492", "locus": "GCA000209735_01485" }, { "type": "cds", "sequence": "contig_1", "start": 827329, "stop": 827895, "strand": "-", "frame": 2, "gene": null, "product": "Glucokinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFS4", "UniRef:UniRef90_C4XFS4" ], "nt": "ATGGCTTTGGCAAATCACAAATATTACAAACAAAAGAAAAGTGATGTAACTCAATTTTTCACAGTATCAACAGGTTTAGGTGCTGGTCTTATTATTAATGATCAAATATATGTTGGTAAAAATCACTTAGCTCAAGAAATCGCGAGAGCACCTTTAGGTACTGATGAAGATGACTCATTTCATTTACTTCCTTATTCAGTGGAATTGTATGCTTCAGGAACAGGCATTGAATTAAGATATAACAAATTAACAGGAGAAAAATTAAACGCTAAAAAAATTTTTGCTTTATATCCAAATAATGAAAATGCAAAAAAAATAATAAATGAAGGTATAGATTCTTTAGCTAGAACTATCTCAACTTCTTTAGCTTTTTTAAATCCAAGTCTTGTTGTTTTTGGCGGTAGTGTTAGTGAACATAATAATTGGTTTATAGAAAAAGCTATTGAAAAAGCTAAAACATATACAATTAAATCTCAATATCAAAATGTAAAATTTGCTTTTGATCCTTATACAGATTTCAGCGCTGTAATGGGTTTATATTGTTTAGCAAAAGATAAATTTAAATAA", "aa": "MALANHKYYKQKKSDVTQFFTVSTGLGAGLIINDQIYVGKNHLAQEIARAPLGTDEDDSFHLLPYSVELYASGTGIELRYNKLTGEKLNAKKIFALYPNNENAKKIINEGIDSLARTISTSLAFLNPSLVVFGGSVSEHNNWFIEKAIEKAKTYTIKSQYQNVKFAFDPYTDFSAVMGLYCLAKDKFK", "aa_hexdigest": "bb9384e04443ebc48cca139096dc696e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 827329, "stop": 828195, "observations": { "mutation": [ 827976, 828141 ], "stop": [ 827976, 828141 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFS4", "query_cov": 1.0, "subject_cov": 0.6394557823129252, "identity": 1.0, "score": 375.0, "evalue": 6.9e-130, "gene_start": 1, "gene_end": 188, "reference_start": 107, "reference_end": 294, "reference_sequence": "MMKIATADIGGTNTRFALFKKNKFWSKVKFETNKNNYRETLNHIAHLCNLHKIECLALCIPGPADYDKGIVLNTPNLPGWVNKNIKKYLFKHTNLKEIITENDANVMALANHKYYKQKKSDVTQFFTVSTGLGAGLIINDQIYVGKNHLAQEIARAPLGTDEDDSFHLLPYSVELYASGTGIELRYNKLTGEKLNAKKIFALYPNNENAKKIINEGIDSLARTISTSLAFLNPSLVVFGGSVSEHNNWFIEKAIEKAKTYTIKSQYQNVKFAFDPYTDFSAVMGLYCLAKDKFK" }, "paralog": false, "identity": 0.9930555555555556, "score": 577.0, "evalue": 3.38e-212, "description": "Internal stop codon at 827976, 828141. Nonsense mutation around 827976, 828141.", "psc": { "uniref90_id": "UniRef90_C4XFS4", "product": "Glucokinase", "uniref50_id": "UniRef50_C4XFS4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFS4", "UniRef:UniRef50_C4XFS4" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFS4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFS4" ], "product": "Glucokinase" } }, "id": "OAIKOHHJFB_1493", "locus": "GCA000209735_01486" }, { "type": "cds", "sequence": "contig_1", "start": 828468, "stop": 828746, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFS5", "UniRef:UniRef90_C4XFS5" ], "nt": "ATGTTGCCTTATATTATTGTTGGCTACAGTCATGAAAAATTAAAATATTTTTCGCATGTCAATATATCGTTAGGAGTAATCTTAATTATCTTAACTATTTCGCAAGTAGCAGTTTCAACTAAAGCAAATTTCCTTATTTATTTCTTAATAAGCTTTGAAATAATTAACGTTATTTGCATAACTATTAGTTTGATTTTTGGCATTATTTTCTTTTTTGATTATCTACAAAGTTTCAAAGAATCTTTATTAATATTTAAACAATATTTAAAAGAACTTTAA", "aa": "MLPYIIVGYSHEKLKYFSHVNISLGVILIILTISQVAVSTKANFLIYFLISFEIINVICITISLIFGIIFFFDYLQSFKESLLIFKQYLKEL", "aa_hexdigest": "dcdbed1e550841e45b35f9fdb5193f66", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 828324, "stop": 828746, "observations": { "mutation": [ 828342, 828345, 828414 ], "stop": [ 828342, 828345, 828414 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFS5", "query_cov": 1.0, "subject_cov": 0.6571428571428571, "identity": 1.0, "score": 167.0, "evalue": 1.3e-51, "gene_start": 1, "gene_end": 92, "reference_start": 49, "reference_end": 140, "reference_sequence": "MNKQKRWWMLLSYVIIGLVLLIINVVLVCCWQKGSGVFISLIGQTILVMLPYIIVGYSHEKLKYFSHVNISLGVILIILTISQVAVSTKANFLIYFLISFEIINVICITISLIFGIIFFFDYLQSFKESLLIFKQYLKEL" }, "paralog": false, "identity": 0.9785714285714285, "score": 258.0, "evalue": 7.13e-90, "description": "Internal stop codon at 828342, 828345, 828414. Nonsense mutation around 828342, 828345, 828414.", "psc": { "uniref90_id": "UniRef90_C4XFS5", "uniref50_id": "UniRef50_C4XFS5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFS5", "UniRef:UniRef50_C4XFS5" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFS5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFS5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10704.786499999991, "isoelectric_point": 7.852652168273926 }, "id": "OAIKOHHJFB_1494", "locus": "GCA000209735_01487" }, { "type": "cds", "sequence": "contig_1", "start": 828861, "stop": 829220, "strand": "-", "frame": 3, "gene": null, "product": "aldehyde dehydrogenase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002E0F0E1", "UniRef:UniRef90_UPI0002E0F0E1" ], "nt": "ATGCAAGAAGAAATTTTCGGGCCGTTGTTGCCTATTATTCCTTATGATAATTTAGATGATTTATTAGTCAAATTTAAATCACTTCCTAAACCTTTAGCTGCTTATTTATTTACTAAAGATAAGAAAATAATAAAGCTATTTAAAGAACAAGTTACTAGTGGAGCTTTAGTAATTAATGATACCATTATGCATATAGCAAATAGTAATTTACCTTTTGGTGGTGTTGGTGATTCTGGCTATGGCAGAAGTCATGGTTTTGAAGGTTTTAAAGCTATGTCAAATCCAAAAGCTATTTACCAAAGAAAAGGTTTTAATTTTAAATTAAATGAGCATCCTTACAGCAAGAAAAAAGAATTCTGA", "aa": "MQEEIFGPLLPIIPYDNLDDLLVKFKSLPKPLAAYLFTKDKKIIKLFKEQVTSGALVINDTIMHIANSNLPFGGVGDSGYGRSHGFEGFKAMSNPKAIYQRKGFNFKLNEHPYSKKKEF", "aa_hexdigest": "1ba18871fb632d394b157164d06658a4", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 828840, "stop": 829379, "observations": { "mutation": [ 828863, 829235 ], "stop": [ 828863, 829235 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0002E0F0E1", "query_cov": 1.0, "subject_cov": 0.664804469273743, "identity": 1.0, "score": 242.0, "evalue": 2.76e-80, "gene_start": 1, "gene_end": 119, "reference_start": 54, "reference_end": 172, "reference_sequence": "MQNEEYIKGNYPKIINQKHFERLKKYLPKDHKINEKTLQIMPYIFETSWNDLIMQEEIFGPLLPIIPYDNLDDLLVKFKSLPKPLAAYLFTKDKKIIKLFKEQVTSGALVINDTIMHIANSNLPFGGVGDSGYGRSHGFEGFKAMSNPKAIYQRKGFNFKLNEHPYSKKKEFWIKKIIK" }, "paralog": false, "identity": 0.9832402234636871, "score": 358.0, "evalue": 1.56e-128, "description": "Internal stop codon at 828863, 829235. Nonsense mutation around 828863, 829235.", "psc": { "uniref90_id": "UniRef90_UPI0002E0F0E1", "product": "aldehyde dehydrogenase family protein", "uniref50_id": "UniRef50_UPI0002E0F0E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0002E0F0E1", "UniRef:UniRef50_UPI0002E0F0E1" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0002E0F0E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002E0F0E1" ], "product": "aldehyde dehydrogenase family protein" } }, "id": "OAIKOHHJFB_1495", "locus": "GCA000209735_01488" }, { "type": "cds", "sequence": "contig_1", "start": 829233, "stop": 829826, "strand": "-", "frame": 3, "gene": null, "product": "Aldehyde dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFS6", "UniRef:UniRef90_C4XFS6" ], "nt": "ATGCCATTAGTTTCAGCTCTTGCAGCAGGCAATAGAGTAATCATTAAACCAAGTGAATTTTCAGTCCACACTTCACAATTATTACAAGAATTAATCAACAATAATTTTGATCCTCAATATGCTTTTGTAGCAGACAGTTCTTTAGAAGTTGCTCAAGAATTGTTGGACTACAAATTTGATTTAATTCATTTCACAGGTAGCACAACAGTTGGAAAAATTATTGCTAAAAAAGCATCTGAAACTTTGACACCAATTACTTTAGAATTAGGTGGGAAGTGCCCTTGCGTTGTTGACAAAAGTCAAGACATAATTTATTCTGCTCAAAAAATTATGGCTGGTAAATTGCTTAATGCAGGTCAAACTTGTATAGCTCCTGATTATGTTTTAATTGATAAAAATAGAATTAACGCATTTAAAGCTGCTTGTTATGTTTATCTTGAAAACGTTTTGCAAAATGAAGAATATATTAAAGGTAATTATCCAAAGATAATTAATCAAAAACATTTTGAAAGATTAAAAAAATATTTACCTAAAGATCATAAAATTAATGAAAAAACATTACAAATAATGCCTTATATTTTTGAAACAAGCTGA", "aa": "MPLVSALAAGNRVIIKPSEFSVHTSQLLQELINNNFDPQYAFVADSSLEVAQELLDYKFDLIHFTGSTTVGKIIAKKASETLTPITLELGGKCPCVVDKSQDIIYSAQKIMAGKLLNAGQTCIAPDYVLIDKNRINAFKAACYVYLENVLQNEEYIKGNYPKIINQKHFERLKKYLPKDHKINEKTLQIMPYIFETS", "aa_hexdigest": "f40943a101ef896de8e230ddca7662bb", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 828930, "stop": 830126, "observations": { "mutation": [ 829235, 829853, 829952 ], "stop": [ 829235, 829853, 829952 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFS6", "query_cov": 1.0, "subject_cov": 0.41041666666666665, "identity": 1.0, "score": 390.0, "evalue": 6.03e-133, "gene_start": 1, "gene_end": 197, "reference_start": 153, "reference_end": 349, "reference_sequence": "MRSKFKITYKFNIIFITKLFIYNILRQTGDIMNAKNVYQLQREKILKNAFIDKKLRKENIEKLYSIIVQNESKIKEALSLDLNKSSLESYFSEIGLVLKEIKFNIKNLNKWTKPFHVSTPSHLALGKSEIQYEPLGQVLIISPWNYPFYLSLMPLVSALAAGNRVIIKPSEFSVHTSQLLQELINNNFDPQYAFVADSSLEVAQELLDYKFDLIHFTGSTTVGKIIAKKASETLTPITLELGGKCPCVVDKSQDIIYSAQKIMAGKLLNAGQTCIAPDYVLIDKNRINAFKAACYVYLENVLQNEEYIKGNYPKIINQKHFERLKKYLPKDHKINEKTLQIMPYIFETSWNDLIMQEEIFGPLLPIIPYDNLDDLLVKFKSLPKPLAAYLFTKDKKIIKLFKEQVTSGALVINDTIMHIANSNLPFGGVGDSGYGRSHGFEGFKAMSNPKAIYQRKGFNFKLNEHPYSKKKEFWIKKIIK" }, "paralog": false, "identity": 0.992462311557789, "score": 777.0, "evalue": 4.3e-288, "description": "Internal stop codon at 829235, 829853, 829952. Nonsense mutation around 829235, 829853, 829952.", "psc": { "uniref90_id": "UniRef90_C4XFS6", "product": "Aldehyde dehydrogenase", "uniref50_id": "UniRef50_C4XFS6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFS6", "UniRef:UniRef50_C4XFS6" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFS6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFS6" ], "product": "Aldehyde dehydrogenase" } }, "id": "OAIKOHHJFB_1496", "locus": "GCA000209735_01489" }, { "type": "cds", "sequence": "contig_1", "start": 829950, "stop": 830189, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCAAAAAATGTTTATCAATTGCAAAGAGAAAAAATATTAAAAAATGCTTTTATTGACAAAAAATTGAGAAAAGAAAATATCGAAAAACTTTACTCAATAATTGTGCAAAATGAAAGTAAAATTAAAGAAGCTTTATCTTTGGACCTTAATAAATCATCACTAGAATCATATTTTAGTGAGATAGGTTTAGTCTTAAAAGAAATCAAATTTAATATTAAAAATTTAAATAAATGA", "aa": "MNAKNVYQLQREKILKNAFIDKKLRKENIEKLYSIIVQNESKIKEALSLDLNKSSLESYFSEIGLVLKEIKFNIKNLNK", "aa_hexdigest": "c0a666b852bfeb01ff9cc9b2984644c7", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9301.804799999993, "isoelectric_point": 9.619085884094236 }, "id": "OAIKOHHJFB_1497", "locus": "GCA000209735_01490" }, { "type": "cds", "sequence": "contig_1", "start": 830549, "stop": 830815, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTCCAGGTCCTAGCGTCATTGAAGCAATTAGTTATATTACTATTAAACTTTTAGGTTTCAAAAAGGGCTTGTTTTTGGCAACTTTTGCAACCTTACCTCATGTTTTAGTTTTCTTTTTAATCTTTTATTTTGTGCAACATATTCCTCAAAAATATTTGTTTGTTATTGAACTTGGTGTTATTGTTGCAATTATTGGATCATTGATTGGTTTTGCTTGGGAATACTTCAAAAAATCAAGAGGCAAAACTAAAATTAGTTTGTGA", "aa": "MLPGPSVIEAISYITIKLLGFKKGLFLATFATLPHVLVFFLIFYFVQHIPQKYLFVIELGVIVAIIGSLIGFAWEYFKKSRGKTKISL", "aa_hexdigest": "1faedfa2cefe365f13dafcceb95d05f2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9952.00599999999, "isoelectric_point": 9.853041648864746 }, "id": "OAIKOHHJFB_1498", "locus": "GCA000209735_01491" }, { "type": "cds", "sequence": "contig_1", "start": 830873, "stop": 831079, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTGTAATAATTATGGTTCTAATTATTTGTATTTTTGCAACTGTTGAAGCAATCAAACACAAAAAAGAAATCAAAAAACTTAAAGAAAATATTGAAAATAAAGTTACTAATTTAGACAATAACATAGAAGAAAAAATAGAACAAAACTTAGAATTAGAGACCAAAGAAAATGATTTGAATAGTGACAAGAAAGGAGGTCAATAA", "aa": "MPVIIMVLIICIFATVEAIKHKKEIKKLKENIENKVTNLDNNIEEKIEQNLELETKENDLNSDKKGGQ", "aa_hexdigest": "e56a278954b69d1ea5da6add22e1ddca", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7834.0481999999965, "isoelectric_point": 5.3715917587280275 }, "id": "OAIKOHHJFB_1499", "locus": "GCA000209735_01492" }, { "type": "cds", "sequence": "contig_1", "start": 831340, "stop": 831603, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTAACTTATTTAGTCTTTGTTATTCCACCAATTTTAATGATGAAATTAGCAATGAAATATTCAAAAAAATTTGAAAATAATAAATATTTAATAAAATTAATTAACATAATGAATCCTGTTGTAACAGGTATTATTGTTGCCTTGGGTTTTCAACTTTTTATAGCTTCAGTAGCACCTCAAGTTTTCTTTAATAAATCATTTACTAACTATGTAGGAATAAATAATGATACACAAAAAGCACTGTTTTATAGGGGTTGA", "aa": "MFLTYLVFVIPPILMMKLAMKYSKKFENNKYLIKLINIMNPVVTGIIVALGFQLFIASVAPQVFFNKSFTNYVGINNDTQKALFYRG", "aa_hexdigest": "ff114127c916604c6f5209d78b9669e8", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10004.98719999999, "isoelectric_point": 9.932079887390138 }, "id": "OAIKOHHJFB_1500", "locus": "GCA000209735_01493" }, { "type": "cds", "sequence": "contig_1", "start": 831785, "stop": 832708, "strand": "+", "frame": 2, "gene": null, "product": "Pseudouridine synthase", "db_xrefs": [ "EC:5.4.99.23", "KEGG:K06180", "RefSeq:WP_013527180.1", "SO:0001217", "UniParc:UPI0001F59955", "UniRef:UniRef100_C4XFS9", "UniRef:UniRef50_Q4A5G9", "UniRef:UniRef90_C4XFS9" ], "nt": "ATGTTAAAAATTAGTGTTCAATATAAAGAGAGAATCGATAAGTATATCGGTCAAAATAGTGAATTAAGTCGAAATGATATTAAAGAGTTAATTGAGCAAGGTGCAGTTTATATTAATGACAACGTTCAAGTACATAAATGTAATTACATCGTTCGCGAAGGACAAGAAATTACAATTGTTAAATTACTTAACAAAGAAATTAAAATTGAACCAGTTAATATTCCTCTTGACATTAAATATGAAGATGATGATTTAGTAATAATTAATAAACCAAGTAATCTAGTTGTCCACCCTTCACCGGGGCATTACAATGACACACTAGTTAATGGACTTTTATATCATTTTAAAAACAATTTATCAAATGAAAATGGGTTACTTCGACCAGGTATAGTACACCGTATTGATAAAGACACAAGTGGCTTATTAATAATTGCTAAAAATAACAAGATGCATAACTCATTAACTGACATGCTCAAAAGACATGAAATTGAAAGAAGTTATTTAGCTATCGTTAGAGGCTTAATTGAAAATCCACTTACTAAAATTAATTTACCAATTGCTAGAAATACTGTTGATCGTAAAATGATGTCAGTTCATAAAGAAGGTAAAGAAGCTATAACCTTTGTTCATTTGCTTAAGCACTTTTATTTAGATAAAGAACCAATGTCTTTAGTTAGATGCGAATTAAAAACAGGTCGAACTCATCAAATTAGAGTCCACTTGTCTTACATCAAACATCCAGTTTACGGTGATCCTACTTATGGCAAAAGTGTTGATGAATTTAATCAAAGATTACATGCTTATAAATTAAAATTTATTCATCCACTTACTAAAAAAGAAATTTGTGTTTATGCACCGGTGCCTAAAGAATTTGATGTAGCCGATTATGATTTTAATGAACTATTAAAAAATAATGTTTTATAA", "aa": "MLKISVQYKERIDKYIGQNSELSRNDIKELIEQGAVYINDNVQVHKCNYIVREGQEITIVKLLNKEIKIEPVNIPLDIKYEDDDLVIINKPSNLVVHPSPGHYNDTLVNGLLYHFKNNLSNENGLLRPGIVHRIDKDTSGLLIIAKNNKMHNSLTDMLKRHEIERSYLAIVRGLIENPLTKINLPIARNTVDRKMMSVHKEGKEAITFVHLLKHFYLDKEPMSLVRCELKTGRTHQIRVHLSYIKHPVYGDPTYGKSVDEFNQRLHAYKLKFIHPLTKKEICVYAPVPKEFDVADYDFNELLKNNVL", "aa_hexdigest": "49276f710a7a9fb172f4c119c7722451", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59955", "ncbi_nrp_id": "WP_013527180.1", "uniref100_id": "UniRef100_C4XFS9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59955", "RefSeq:WP_013527180.1", "UniRef:UniRef100_C4XFS9" ] }, "ips": { "uniref100_id": "UniRef100_C4XFS9", "uniref90_id": "UniRef90_C4XFS9" }, "psc": { "uniref90_id": "UniRef90_C4XFS9", "product": "Pseudouridine synthase", "ec_ids": [ "5.4.99.23" ], "uniref50_id": "UniRef50_Q4A5G9", "kegg_orthology_id": "K06180" }, "pscc": { "uniref50_id": "UniRef50_Q4A5G9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5G9" ], "product": "Pseudouridine synthase" }, "genes": [], "id": "OAIKOHHJFB_1501", "locus": "GCA000209735_01494" }, { "type": "cds", "sequence": "contig_1", "start": 832879, "stop": 833088, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTCGCTGCTTTTAAAGATAATTTAGTTGACTATATTGTAAAAATTAATGGTGGTACAACTCCAAGTTTTAAACCTGAAGATCAAATAATGAGCATGATTATTACAAATGGTTTAGTTACAGTTGGTATGTTAGTTAGCATTATTGTATTTTTAGTTTTTTATGCTCGAACATTTAAAGAAAAATCATTAGCCAACATGCATACATGA", "aa": "MLAAFKDNLVDYIVKINGGTTPSFKPEDQIMSMIITNGLVTVGMLVSIIVFLVFYARTFKEKSLANMHT", "aa_hexdigest": "62d54fabd05170d1bf1e5153377c760e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7685.072599999998, "isoelectric_point": 8.085834312438966 }, "id": "OAIKOHHJFB_1502", "locus": "GCA000209735_01495" }, { "type": "cds", "sequence": "contig_1", "start": 833125, "stop": 833685, "strand": "+", "frame": 1, "gene": null, "product": "Rho termination factor N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFT0", "UniRef:UniRef90_C4XFT0" ], "nt": "TTGTTCTCAATTATTAGAAACATTAGATCACTAGCTACTGCTTACAATCCAATTATTTTATCAATAACAATAGTTACTTCAGTTATCACAGTTGTTTTAATTGTTTTCCAATTCTTGTTTGTTAGTCAATTAAGATACTTCAAAGGTGTATTTATTAATGCAAGAAGATTAGAAGAAATCAAACAACTTCAAAAAAAGATGAAAGAATCAGGTATGATGGATGAAAGCAATTTAGCAAACAATCTTTTTGGAATCATGTTAGATGATGTTGATGATGTAAGTGCTAATCAAGATAAAAGCAAATTCAATAAAGAAGAAGATATTGAAGAAGCTGAAATAGTTGATAACAAAGCACATCTTAAAAATAATGAAAAAGAAAATGAAGAAAAAATCAAAAAATTATTAGCATTGCCTAATGCAAAACTTTACAACATAGCAGAAACCCTATACATTAGTGGTTACAAAAAAATGGACAAAGAAGAATTATGCCGCTTAATTGTTCAAATTGTTGATACTCAAAAGGCTAAAGAAGACGAAGAAGCAAACAAAGACAAAAAATAA", "aa": "MFSIIRNIRSLATAYNPIILSITIVTSVITVVLIVFQFLFVSQLRYFKGVFINARRLEEIKQLQKKMKESGMMDESNLANNLFGIMLDDVDDVSANQDKSKFNKEEDIEEAEIVDNKAHLKNNEKENEEKIKKLLALPNAKLYNIAETLYISGYKKMDKEELCRLIVQIVDTQKAKEDEEANKDKK", "aa_hexdigest": "6f9b50f0732cacbd0a937a46baf315c2", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 832825, "stop": 833685, "observations": { "mutation": [ 833086, 833113 ], "stop": [ 833086, 833113 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFT0", "query_cov": 1.0, "subject_cov": 0.577639751552795, "identity": 0.995, "score": 329.0, "evalue": 2.11e-111, "gene_start": 1, "gene_end": 186, "reference_start": 137, "reference_end": 322, "reference_sequence": "MDIGEKMNLFDKKYLQIKSTNKLWALEKERFRPVVILSLLTFFLALACALTSTILLAAFKDNLVDYIVKINGGTTPSFKPEDQIMSMIITNGLVTVGMLVSIIVFLVFYARTFKEKSLANMHTWPSMVLIISWFINLFSIIRNIRSLATAYNPIILSITIVTSVITVVLIVFQFLFVSQLRYFKGVFINARRLEEIKQLQKKMKESGMMDESNLANNLFGIMLDDVDDVSANQDKSKFNKEEDIEEAEIVDNKAHLKNNEKENEEKIKKLLALPNAKLYNIAETLYISGYKKMDKEELCRLIVQIVDTQKAKEDEEANKDKK" }, "paralog": false, "identity": 0.993006993006993, "score": 539.0, "evalue": 1.04e-196, "description": "Internal stop codon at 833086, 833113. Nonsense mutation around 833086, 833113.", "psc": { "uniref90_id": "UniRef90_C4XFT0", "product": "Rho termination factor N-terminal domain-containing protein", "uniref50_id": "UniRef50_C4XFT0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFT0", "UniRef:UniRef50_C4XFT0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFT0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFT0" ], "product": "Rho termination factor N-terminal domain-containing protein" } }, "id": "OAIKOHHJFB_1503", "locus": "GCA000209735_01496" }, { "type": "cds", "sequence": "contig_1", "start": 833739, "stop": 834335, "strand": "+", "frame": 3, "gene": null, "product": "ribonuclease HII", "db_xrefs": [ "EC:3.1.26.4", "GO:0003723", "GO:0004523", "KEGG:K03470", "RefSeq:WP_013355014.1", "SO:0001217", "UniParc:UPI0001E33051", "UniRef:UniRef100_C4XFT1", "UniRef:UniRef50_I5D6C7", "UniRef:UniRef90_C4XFT1" ], "nt": "ATGTTGAATTATGAAAAAGAAAATTATCCTGACACTAAATTAATTGTAGGCTTAGATGAAGCAGGAAGAGGTTGTTGTGCAGGGCCTTTAGTAGTTGGTTGTGTTATTATGCCTCAAAATTTTAACCATCCTTTAATAAAGGATTCTAAACAATTGAGTCAAAAACAACGTGAGGAAGCGTTAGAAATTATTCTTGAAAATGCTATTGAAACAGTAACAACTATTATTAATGCTCAAGAAGTAGATCAACTAAATCCAAAGCAAGCTTCAAGAATTGGCATGCAAAGGTGTATTAATAAACTTAATACAAAACCTGAATTAGTAATTACAGATTTTGAAAAAATTAATGACATCAATATACCTCAAATTAATTTAGTAAAAGGTGACCAATTAAGTATTAATGTGGCAGCAGCTTCAATTTTAGCTAAAGTAACTAGGGACTTAATTATGGTTGGAATTGACAAAAAATTTCCTATGTATAATTTCAAGAAAAATAAGGGCTATTGTACAAAAGAACATGAAGCAGCAATGAGTCAATATGGCATCTGTGAATTTCATAGAAAGTCATATAAAAATGTTAAAAAATATTTGAATTAA", "aa": "MLNYEKENYPDTKLIVGLDEAGRGCCAGPLVVGCVIMPQNFNHPLIKDSKQLSQKQREEALEIILENAIETVTTIINAQEVDQLNPKQASRIGMQRCINKLNTKPELVITDFEKINDINIPQINLVKGDQLSINVAAASILAKVTRDLIMVGIDKKFPMYNFKKNKGYCTKEHEAAMSQYGICEFHRKSYKNVKKYLN", "aa_hexdigest": "4b51a8bd10e0b19c82873a8af19a91ac", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E33051", "ncbi_nrp_id": "WP_013355014.1", "uniref100_id": "UniRef100_C4XFT1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33051", "RefSeq:WP_013355014.1", "UniRef:UniRef100_C4XFT1" ] }, "ips": { "uniref100_id": "UniRef100_C4XFT1", "uniref90_id": "UniRef90_C4XFT1" }, "psc": { "uniref90_id": "UniRef90_C4XFT1", "product": "ribonuclease HII", "ec_ids": [ "3.1.26.4" ], "uniref50_id": "UniRef50_I5D6C7", "kegg_orthology_id": "K03470", "go_ids": [ "GO:0003723", "GO:0004523" ] }, "pscc": { "uniref50_id": "UniRef50_I5D6C7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D6C7" ], "product": "Ribonuclease HII" }, "genes": [], "id": "OAIKOHHJFB_1504", "locus": "GCA000209735_01497" }, { "type": "cds", "sequence": "contig_1", "start": 834531, "stop": 834863, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAATTGCTCTAAGTGGCAAAACAGTTTTAAATGAAAAATTTAATGAAATTGTTCAACAAAAAATTGAATTAGAAAAGAAATATGGAGCATTTATTTACTTTGATGTAATTGTTAAAGAAGAAACTTTAACCTTAGATAATTGTGACTACCAAGTTATCATGGAAACATTCAAACCAAATGAAATTGCTAAAATTAATTTAGTTCCTAAAGACTATATTCCAGCTTTTGATCCTTTACCAATTAATAAATTAGAAGAATATGCAATGGAATATAATCCGGGCGGTTCAAAAAATTTAGCAAAAGAAGAATTAAATGAAATTAAAAGATGA", "aa": "MLIALSGKTVLNEKFNEIVQQKIELEKKYGAFIYFDVIVKEETLTLDNCDYQVIMETFKPNEIAKINLVPKDYIPAFDPLPINKLEEYAMEYNPGGSKNLAKEELNEIKR", "aa_hexdigest": "5929d454884129dd65bcfe5a2b6dc57d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12746.611199999985, "isoelectric_point": 4.814740180969238 }, "id": "OAIKOHHJFB_1505", "locus": "GCA000209735_01498" }, { "type": "cds", "sequence": "contig_1", "start": 835050, "stop": 835154, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGAAATCAATTTAGTATCAAGATTTATTGATAAAAAAATCAAACTTGAACACTTGTCTTTTGAAAATGAAGTAGAAAAACATGATGCATTAAGACAATGA", "aa": "MNEINLVSRFIDKKIKLEHLSFENEVEKHDALRQ", "aa_hexdigest": "1b6b5d68af35965b1a0d7484f9e95523", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4124.677600000001, "isoelectric_point": 6.028479957580566 }, "id": "OAIKOHHJFB_1506", "locus": "GCA000209735_01499" }, { "type": "cds", "sequence": "contig_1", "start": 835596, "stop": 836180, "strand": "-", "frame": 3, "gene": null, "product": "Zinc ribbon domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFT3", "UniRef:UniRef90_C4XFT3" ], "nt": "ATGGAATGCAAATCATGTGGAGCTAATGTTAGAATCACAGATAAATTTTGTTCTTTTTGCCGATCATTAATCGAAGCACCTAAATCAAGCGTTGAAACATCTCAACACACAACTGCAACTACAAGAGTAGTAACAGAATCAAAAGCAATTAATTATGATGTATTGAACTTATTAGTTATAGAAGATAAATATGACGAAATTGATTTTGATTTATCTTCTAACACAGCTGCAAACTCAATGAAAACATATGTAAACAAAGGAGGAAGCATTTCAGAACTTGAGAAAAGTTATGTTTTAGCAAATAAAATCGATTACTATGAATCACTTGACAAAGATGAAATCTTAAAAACTTACTCAGAAAAAATTAAGAAATATTCCGGTAAAGATATTTGTTGTTTAAAAAGAAGTGAATTACAAGCCTTAGAAAGAAAAATGAGTTTATATGAATTCAATGATTATCTTAAAGTATTAAAAGATAGAGTATTTAATAAAGAAGGAATTATGCTAAGTGACAGCGACCTACTCGACAAAATAAACTCAGATAGAAACATTGAAGTTTTTGCTATTTGTTCTTATGACTTCTGA", "aa": "MECKSCGANVRITDKFCSFCRSLIEAPKSSVETSQHTTATTRVVTESKAINYDVLNLLVIEDKYDEIDFDLSSNTAANSMKTYVNKGGSISELEKSYVLANKIDYYESLDKDEILKTYSEKIKKYSGKDICCLKRSELQALERKMSLYEFNDYLKVLKDRVFNKEGIMLSDSDLLDKINSDRNIEVFAICSYDF", "aa_hexdigest": "e1ba4f6eb62a89dc85fabb2ea897b1f5", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 835383, "stop": 836183, "observations": { "mutation": [ 835496, 835598 ], "stop": [ 835496, 835598 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFT3", "query_cov": 1.0, "subject_cov": 0.7293233082706767, "identity": 1.0, "score": 373.0, "evalue": 1.91e-129, "gene_start": 1, "gene_end": 194, "reference_start": 2, "reference_end": 195, "reference_sequence": "MMECKSCGANVRITDKFCSFCRSLIEAPKSSVETSQHTTATTRVVTESKAINYDVLNLLVIEDKYDEIDFDLSSNTAANSMKTYVNKGGSISELEKSYVLANKIDYYESLDKDEILKTYSEKIKKYSGKDICCLKRSELQALERKMSLYEFNDYLKVLKDRVFNKEGIMLSDSDLLDKINSDRNIEVFAICSYDFWDKKTVELDRQNKVEYEQRIKRINNKKKTAKVIIWTIVGIFISIFIICFLVALGVTIRSSVSIASTSSSYY" }, "paralog": false, "identity": 0.9887218045112782, "score": 511.0, "evalue": 2.35e-186, "description": "Internal stop codon at 835496, 835598. Nonsense mutation around 835496, 835598.", "psc": { "uniref90_id": "UniRef90_C4XFT3", "product": "Zinc ribbon domain-containing protein", "uniref50_id": "UniRef50_C4XFT3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFT3", "UniRef:UniRef50_C4XFT3" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFT3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFT3" ], "product": "Zinc ribbon domain-containing protein" } }, "id": "OAIKOHHJFB_1507", "locus": "GCA000209735_01500" }, { "type": "cds", "sequence": "contig_1", "start": 836183, "stop": 836281, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAAAACAGATCAAAAATTTAAATCAAGAGAAAGATCACTTAGAGAAGAATACATCACAGGACAAAAACCTAAAGATGAAGAAGGGTTGTACTAA", "aa": "MSKTDQKFKSRERSLREEYITGQKPKDEEGLY", "aa_hexdigest": "a36ef443794295c5669fa644944a48b5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3878.2836000000007, "isoelectric_point": 8.065011024475098 }, "id": "OAIKOHHJFB_1508", "locus": "GCA000209735_01501" }, { "type": "cds", "sequence": "contig_1", "start": 836730, "stop": 837170, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13240.11", "PFAM:PF17032.10", "PFAM:PF13248.11" ], "nt": "ATGGAAGTTGTTAACTCAAGCATTGAAGATATTAGTATACCTAAAGAGCAACAAGATGAATTGAACAAAATTCTTAAAAGAAAAGCCGAAGTTGATATCATGGGAGACGACTATGCTAAAGTTAGAGGCTATGACGTTTACCAAGATGCAGCAAACAATTCGGGTTCAGTAGGAACTTTTTTTGGAGCAGGCATTGGTTTAGGACTAAACGAAGGTGCTTCAAATTTATTAAGTGATAGCAAAATCAATAAATCAGCAACCCCAACAGCAGCAGCTGCATTATTAATCACTTGCGGTACATGTGGAAGCAAAGTATCTAAAGATTCTAAATTTTGTTTTGAATGTGGTTCAAAAATCGCAACAAACAAATGTTCATCATGTAATATCGACTTATCGCCAAATGCAAAATTCTGCGTAAATTGCGGAAAGAAGGTAAAATAA", "aa": "MEVVNSSIEDISIPKEQQDELNKILKRKAEVDIMGDDYAKVRGYDVYQDAANNSGSVGTFFGAGIGLGLNEGASNLLSDSKINKSATPTAAAALLITCGTCGSKVSKDSKFCFECGSKIATNKCSSCNIDLSPNAKFCVNCGKKVK", "aa_hexdigest": "98759b982d361b41e92adc86842ff972", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13240.11", "name": "Zn_Ribbon_1", "length": 23, "aa_cov": 0.15753424657534246, "hmm_cov": 1.0, "evalue": 2.520921899391168e-12, "score": 42.3330192565918, "start": 123, "stop": 145 }, { "id": "PF17032.10", "name": "Zn_ribbon_15", "length": 22, "aa_cov": 0.1506849315068493, "hmm_cov": 1.0, "evalue": 2.5439764374307624e-08, "score": 30.724994659423828, "start": 95, "stop": 116 }, { "id": "PF13248.11", "name": "Zn_ribbon_3", "length": 24, "aa_cov": 0.1643835616438356, "hmm_cov": 1.0, "evalue": 3.114768443717588e-09, "score": 32.15855026245117, "start": 96, "stop": 119 } ], "seq_stats": { "molecular_weight": 15387.314299999987, "isoelectric_point": 7.450250434875489 }, "id": "OAIKOHHJFB_1509", "locus": "GCA000209735_01502" }, { "type": "cds", "sequence": "contig_1", "start": 838648, "stop": 839382, "strand": "-", "frame": 2, "gene": null, "product": "transketolase-like TK C-terminal-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0026B1FBDD" ], "nt": "ATGCAAAAAATAGATCAAAATTACAAAAACGTTTTAGGTGGTTCAGCTGACTTAGCCTCAGCTACAAAAGTTAACTTTAGCAAATCTTATGCTGAAGGTGGCAAAAACATTCACTATGGAATTAGAGAATTTGCTATGACTGCTATAAATAATGGAATCTATTTACACTCACATTTAAGAACAATAGATTCAACATTCTTAGCTTTTAGCGATTATGCTAAAGGTGCTCTAAGACTAGGTTCAATTATGAAAATACCTTCAGTTCATGTTTACACTCATGATAGTTACCAAGTTGGGGGTGACGGACCAACTCATCAACCAGTAGAACAATTAGCAATGCTTAGAAGTACTCCAAATTTTAAAGTTATTAGACCTATGGATGAGAGCGAAGTCAAAGCAGCATTCAATTATGCATTAAATCAAAATGAAAATCAAGTAGCTATTATTGGTTGTCGTCAAAATGTTAAATCTTACAATTTACATCCTGAAGGTGAATTGCAACCAGCTTACATTATTCGTCCTGCAAAAAACCATGACATTAGTATTTTGGCTTCTGGTAGTGAAGTTGACTTAGCAGAAAAGGTTGCTAATAAATTAGTTGGAGCAGGCATTAAAGCTCAAGTTATTTCCGTTCCTTTATTACAAGATTTAGTTAATGATGAAAAATTAATCAAAAGCTTAGGCATTGACAAAAAACCAATATATGCTATTGAAGCTTCAACAGATCCATTGTGA", "aa": "MQKIDQNYKNVLGGSADLASATKVNFSKSYAEGGKNIHYGIREFAMTAINNGIYLHSHLRTIDSTFLAFSDYAKGALRLGSIMKIPSVHVYTHDSYQVGGDGPTHQPVEQLAMLRSTPNFKVIRPMDESEVKAAFNYALNQNENQVAIIGCRQNVKSYNLHPEGELQPAYIIRPAKNHDISILASGSEVDLAEKVANKLVGAGIKAQVISVPLLQDLVNDEKLIKSLGIDKKPIYAIEASTDPL", "aa_hexdigest": "5fe999d8aafce3b0e00b39e66143a0c7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003080BCD6", "query_cov": 0.9590163934426229, "subject_cov": 0.8447653429602888, "identity": 0.581, "score": 247.0, "evalue": 9.65e-79, "valid": false, "product": "transketolase-like TK C-terminal-containing protein", "uniref50_id": "UniRef50_UPI0026B1FBDD" }, "pscc": { "uniref50_id": "UniRef50_UPI0026B1FBDD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0026B1FBDD" ], "product": "transketolase-like TK C-terminal-containing protein" }, "genes": [], "id": "OAIKOHHJFB_1510", "locus": "GCA000209735_01503" }, { "type": "cds", "sequence": "contig_1", "start": 839530, "stop": 840240, "strand": "-", "frame": 2, "gene": null, "product": "1-deoxy-D-xylulose-5-phosphate synthase N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF16CA3" ], "nt": "ATGTCATATTATTCAATTATGCATTTCTTAGGTTTATTAGCAAAAGAAGACATGCAAAATCATAAAAAAATGGACTCAAAAACACCTTCTCATCCTGAGTTAGAATATACACCTTTTGTGGATGCTTCAACAGGTCCTTTAGGTCAAGGAATAGCTATGGCTACTGGTATGGCTCTTAGTCTAAAAGTACTACAAAACAGGTTTAATCGTAAAAATTACAATATTATAGACCACTACATTTATGCGCTTCATGTAGACGGTTGTATTCAAGAAGGTGTGGCTCATGAAGCTATTCAATTTGCAGGTACAAATCAATTAGATAGACTTATTTTAATTCATGATTTTAATAATGCTCAAATTGATACAAAAACCGAGGTTGTTAATAATATTAACTTCATTAAATATTTTGAAGCTTGTCATTTTGCAACCTTTGTAGTTAAAGAAGATAAACCTGAATTAATTCTTGAAGCAATTCAAAAAGCTAAAGCTTCTAAGAAACCTTCATACATTCAAGTTCACACAGCAATAGCTAAAAATACACCTTTTGAAAACTCGCCAAAAGGTCACCATGGAATGTTAAAAGAAGAAGATACTATTGAATTTAAAAAGAAATGTGGTTTAACTTCATTTGTTCCTTTTGAATATGACAAAGAAGTTTATGACTATGGAGAAGAATTATTAAAAGAAAAAGACAAACATTATAAAGAATGA", "aa": "MSYYSIMHFLGLLAKEDMQNHKKMDSKTPSHPELEYTPFVDASTGPLGQGIAMATGMALSLKVLQNRFNRKNYNIIDHYIYALHVDGCIQEGVAHEAIQFAGTNQLDRLILIHDFNNAQIDTKTEVVNNINFIKYFEACHFATFVVKEDKPELILEAIQKAKASKKPSYIQVHTAIAKNTPFENSPKGHHGMLKEEDTIEFKKKCGLTSFVPFEYDKEVYDYGEELLKEKDKHYKE", "aa_hexdigest": "34dd8043a4ed537bef09358c92c9ec4f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI002FF16CA3", "query_cov": 0.809322033898305, "subject_cov": 0.880184331797235, "identity": 0.508, "score": 201.0, "evalue": 1.57e-61, "valid": false, "product": "1-deoxy-D-xylulose-5-phosphate synthase N-terminal domain-containing protein", "uniref50_id": "UniRef50_UPI002FF16CA3" }, "pscc": { "uniref50_id": "UniRef50_UPI002FF16CA3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF16CA3" ], "product": "1-deoxy-D-xylulose-5-phosphate synthase N-terminal domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_1511", "locus": "GCA000209735_01504" }, { "type": "cds", "sequence": "contig_1", "start": 840283, "stop": 840453, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00456.26" ], "nt": "ATGAAAAATAATTTAATCGAAAATAAATTAATTGCTTCAATGCGCGGCATAGCATTGGATTCAATCAACAAAGCAAAAGGTGGCCACATTGGTATGGCTATTGGAGCTGCAAATATTACTTATTCATTATTAGGAAAGGAATTAAGATTCTCAAAAGAAGATCCGAAATGA", "aa": "MKNNLIENKLIASMRGIALDSINKAKGGHIGMAIGAANITYSLLGKELRFSKEDPK", "aa_hexdigest": "6d4df5968824f334d0f4f49936664834", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00456.26", "name": "Transketolase_N", "length": 49, "aa_cov": 0.875, "hmm_cov": 1.0, "evalue": 2.3258246914820337e-08, "score": 29.43653106689453, "start": 7, "stop": 55 } ], "seq_stats": { "molecular_weight": 6043.0491999999995, "isoelectric_point": 9.775808525085449 }, "id": "OAIKOHHJFB_1512", "locus": "GCA000209735_01505" }, { "type": "cds", "sequence": "contig_1", "start": 840552, "stop": 840665, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGATTTTAGAGATGTAGCAGATCGCGACATTAGATTAAACAGATTTATCAACGGAATGATTTATGTATCAGATGGTTGCGTAAAAAATCAACAAGAACTATATGATTGA", "aa": "MSDFRDVADRDIRLNRFINGMIYVSDGCVKNQQELYD", "aa_hexdigest": "bd75a301a6f1d5c4e7105b81e844b134", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4397.880000000001, "isoelectric_point": 4.658887672424316 }, "id": "OAIKOHHJFB_1513", "locus": "GCA000209735_01506" }, { "type": "cds", "sequence": "contig_1", "start": 840666, "stop": 841004, "strand": "-", "frame": 3, "gene": null, "product": "Nucleoside 2-deoxyribosyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFT8", "UniRef:UniRef90_C4XFT8" ], "nt": "ATGAAAAAAATACATGCTTATGTAGCTGGTCCATTGTTTACTAGAGCAGAAATTGACTTAAGATACGCAATCGAAGAAACAATGAAAAAAGCACTAAAATCAAAAGAATTAAAAGGCAAAATTGACTTTGACATTTTCAATCCGATTCATCTAAATGAAGAATTAGAACAAAACGGAAAATTAACACCACAAGAAATTTTCAAAAATGACTTAGCAGCAATTCAAAAAAGTAAACTTACAATTCTTGACATTGACAACAAAGATGATGGCACCATGGCAGAATTTGGTTACTTCTTAGCAATGAAAGAAAGAGATCCAGAAGTTAAAATATGTGTTTGA", "aa": "MKKIHAYVAGPLFTRAEIDLRYAIEETMKKALKSKELKGKIDFDIFNPIHLNEELEQNGKLTPQEIFKNDLAAIQKSKLTILDIDNKDDGTMAEFGYFLAMKERDPEVKICV", "aa_hexdigest": "5019ddcd36705ab0d43f0b32dc87cfb5", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 840531, "stop": 841004, "observations": { "mutation": [ 840554, 840668 ], "stop": [ 840554, 840668 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFT8", "query_cov": 1.0, "subject_cov": 0.7133757961783439, "identity": 1.0, "score": 223.0, "evalue": 4.14e-73, "gene_start": 1, "gene_end": 112, "reference_start": 1, "reference_end": 112, "reference_sequence": "MKKIHAYVAGPLFTRAEIDLRYAIEETMKKALKSKELKGKIDFDIFNPIHLNEELEQNGKLTPQEIFKNDLAAIQKSKLTILDIDNKDDGTMAEFGYFLAMKERDPEVKICVWMSDFRDVADRDIRLNRFINGMIYVSDGCVKNQQELYDWLIKAYK" }, "paralog": false, "identity": 0.9872611464968153, "score": 308.0, "evalue": 3.28e-109, "description": "Internal stop codon at 840554, 840668. Nonsense mutation around 840554, 840668.", "psc": { "uniref90_id": "UniRef90_C4XFT8", "product": "Nucleoside 2-deoxyribosyltransferase", "uniref50_id": "UniRef50_C4XFT8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFT8", "UniRef:UniRef50_C4XFT8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFT8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFT8" ], "product": "Nucleoside 2-deoxyribosyltransferase" } }, "id": "OAIKOHHJFB_1514", "locus": "GCA000209735_01507" }, { "type": "cds", "sequence": "contig_1", "start": 841536, "stop": 841649, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATAAGACATCAACAAGAAATTGCGGCTTTATATAAAAAGAATAATATAAATCCTCTTGATGCTTTCTTGAATATAATTATTTCCTTCCCTATCTTCATTGCTATGTGA", "aa": "MKIRHQQEIAALYKKNNINPLDAFLNIIISFPIFIAM", "aa_hexdigest": "b7c63e99f55e16ffee088a48f7a7973d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4330.1669, "isoelectric_point": 9.522834587097169 }, "id": "OAIKOHHJFB_1515", "locus": "GCA000209735_01508" }, { "type": "cds", "sequence": "contig_1", "start": 841914, "stop": 842570, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCGAGTCTGCAACATATAAACCTACCTACACAACACCTTATAGTGAAATTAAATTTATTGACCCACAATTCAATTTAAATTCAATTTTATCTTTAGTTGATCAAAAAAATCCAGATCTTTATGAAGTTAAAAAAGATCGTTTAGAAAATAAAACATTCAAAACCCCTAAAAAAGCAACATTGTTTTCAATAGCTACACCAGACCAATTGAAAGCAAAAAATAAAATTGCTGGTAAGGAACTTAGTGAAATTGAGCTTTCAAAACAATACTATGCTCGTGATGTTTTAGAATTTTTATATAGAAAAACATTTATTGAACAAGTAAATGGTAAAGATAATTATTTTGCTAGAAGATTCAAACAAGAGTTTGGTACCGATAAATACAATGATGTATTAGGCAAAATAATTGATCCTACAAATACTAACAAATATAGTAAAAAACAAGTGGAACTTATTTATGAATATGTAAATATATTAAATGGATATCTTAAAACTACAGGTTTCACAAATATTTATGACGACACTCAATTAACAAAAGTTGAATTAGCTAAAGGAACTGAAAAAGTTAAAATAGCTAAAGAAAATGCTTATCTTTCTCCTGCTCCAAAACTTGTATACACTCATAGTGAACCATTCAAACCTTTTGTTAAATGA", "aa": "MSESATYKPTYTTPYSEIKFIDPQFNLNSILSLVDQKNPDLYEVKKDRLENKTFKTPKKATLFSIATPDQLKAKNKIAGKELSEIELSKQYYARDVLEFLYRKTFIEQVNGKDNYFARRFKQEFGTDKYNDVLGKIIDPTNTNKYSKKQVELIYEYVNILNGYLKTTGFTNIYDDTQLTKVELAKGTEKVKIAKENAYLSPAPKLVYTHSEPFKPFVK", "aa_hexdigest": "ab30415c63ae82051cba3bfaaa97e8e3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25326.61180000002, "isoelectric_point": 9.24284839630127 }, "id": "OAIKOHHJFB_1516", "locus": "GCA000209735_01509" }, { "type": "cds", "sequence": "contig_1", "start": 842664, "stop": 842951, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATCAAGACCTCAACATATACAGGTGCAGGTACTGAATATTACACATCATACGATAAAATTAGTCCTTATGTAACATCACTTGAACCAGCTAAAGCTGATAAAAAAATCGACAAAGATGACAAGGCTTTACAACCTGGTGAATTCAAGAGAATTGTTAAAAATGATTCTACAAACTACCATGCTAGCTACAAAAATCATGCCGAAATACTTAAAAAGTTAAGAGAGCAAACAGGCGGAGATGTTTATGGAAAATATAACTCATATTCATCTTCTATTCAATGA", "aa": "MVIKTSTYTGAGTEYYTSYDKISPYVTSLEPAKADKKIDKDDKALQPGEFKRIVKNDSTNYHASYKNHAEILKKLREQTGGDVYGKYNSYSSSIQ", "aa_hexdigest": "127ac08dfb145a7c55058a67d232ff9e", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10740.811799999998, "isoelectric_point": 8.981364631652834 }, "id": "OAIKOHHJFB_1517", "locus": "GCA000209735_01510" }, { "type": "cds", "sequence": "contig_1", "start": 843094, "stop": 843345, "strand": "-", "frame": 2, "gene": "rnpA", "product": "ribonuclease P protein component", "db_xrefs": [ "EC:3.1.26.5", "GO:0000049", "GO:0004526", "GO:0008033", "SO:0001217", "UniRef:UniRef50_A0A449BAF4", "UniRef:UniRef90_C4XFU0" ], "nt": "ATGATTATTTATTACATGCCTAACGATGATTTCAAAATTGGTATAACTGTACCGAAAAAGTTTTGCAATGCAGTTTTAAGAAATTACTATAAACGCCAATTAAAATCAATTATCCATTCTTTGAATTTGTATGGCGAAAAAATGCATTTTGTCTTAATTCCAAGAAAAGATTTTTTGAATTTAATATATGAAGATAAATTGACTATAACTAAAAAATTATTTGAAAAGATTAAAAATGGAAAACAAAAATAG", "aa": "MIIYYMPNDDFKIGITVPKKFCNAVLRNYYKRQLKSIIHSLNLYGEKMHFVLIPRKDFLNLIYEDKLTITKKLFEKIKNGKQK", "aa_hexdigest": "8dcf24da0d4ca8bfcb2e97075703a42c", "start_type": "ATG", "rbs_motif": null, "genes": [ "rnpA" ], "truncated": "3-prime", "pseudogene": { "start": 843094, "stop": 843438, "observations": { "mutation": [ 843396 ], "stop": [ 843396 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFU0", "query_cov": 1.0, "subject_cov": 0.7280701754385965, "identity": 1.0, "score": 171.0, "evalue": 8.27e-54, "gene_start": 1, "gene_end": 83, "reference_start": 32, "reference_end": 114, "reference_sequence": "MVYKMKKIYRLQKSWEFDAVIKFQKQVLNKYMIIYYMPNDDFKIGITVPKKFCNAVLRNYYKRQLKSIIHSLNLYGEKMHFVLIPRKDFLNLIYEDKLTITKKLFEKIKNGKQK" }, "paralog": false, "identity": 0.9824561403508771, "score": 228.0, "evalue": 1.51e-78, "description": "Internal stop codon at 843396. Nonsense mutation around 843396.", "psc": { "uniref90_id": "UniRef90_C4XFU0", "gene": "rnpA", "product": "ribonuclease P protein component", "ec_ids": [ "3.1.26.5" ], "uniref50_id": "UniRef50_A0A449BAF4", "go_ids": [ "GO:0000049", "GO:0004526", "GO:0008033" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFU0", "EC:3.1.26.5", "UniRef:UniRef50_A0A449BAF4", "GO:0000049", "GO:0004526", "GO:0008033" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449BAF4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449BAF4" ], "product": "Ribonuclease P protein component" } }, "id": "OAIKOHHJFB_1518", "locus": "GCA000209735_01511" }, { "type": "cds", "sequence": "contig_1", "start": 843443, "stop": 843592, "strand": "-", "frame": 1, "gene": "rpmH", "product": "50S ribosomal protein L34", "db_xrefs": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02914", "RefSeq:WP_013355004.1", "SO:0001217", "UniParc:UPI0001A8C0FF", "UniRef:UniRef100_C4XFU1", "UniRef:UniRef50_P05647", "UniRef:UniRef90_C4XFU1" ], "nt": "ATGAGTTTAAGAACATATCAACCAAACAAGACCCAACACATCAAGACTCATGGTTTTAGAGCAAGAATGGCAACAACAAATGGTAGAAAAGTTTTAGCTGCTAGAAGAGCTAAAGGTAGAGCAAGACTTACAGTTTCAGATGCTAAATAA", "aa": "MSLRTYQPNKTQHIKTHGFRARMATTNGRKVLAARRAKGRARLTVSDAK", "aa_hexdigest": "5c5a01ca21600f52eea0725329a3cdee", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C0FF", "ncbi_nrp_id": "WP_013355004.1", "uniref100_id": "UniRef100_C4XFU1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C0FF", "RefSeq:WP_013355004.1", "UniRef:UniRef100_C4XFU1" ] }, "ips": { "uniref100_id": "UniRef100_C4XFU1", "uniref90_id": "UniRef90_C4XFU1" }, "psc": { "uniref90_id": "UniRef90_C4XFU1", "gene": "rpmH", "product": "50S ribosomal protein L34", "uniref50_id": "UniRef50_P05647", "kegg_orthology_id": "K02914", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P05647", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P05647" ], "product": "Large ribosomal subunit protein bL34" }, "genes": [ "rpmH" ], "id": "OAIKOHHJFB_1519", "locus": "GCA000209735_01512" }, { "type": "cds", "sequence": "contig_1", "start": 843854, "stop": 844735, "strand": "+", "frame": 2, "gene": "serS", "product": "serine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.11", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434", "KEGG:K01875", "SO:0001217", "UniRef:UniRef50_Q6KIJ8", "UniRef:UniRef90_C4XFU2" ], "nt": "ATGCTAAATTTAAAATACGTTTTAGAAAATGAAAAGAAAGTTAAAGAAGCTTTAAAAAATAGAAATTTCGATCTAAAGCAATTTAATGAGCTTATTAAAGAAGCAAATAAGCGTGGAAAGCTAATGTTTGAAGCTCAAAGCAAGAAAGCTGAATTAACAAAATTTAGTAAAGAATTTGCAGCTAACAAAAACAATAAAACTAAATTAGCTAAATTACAAAAAGAAGTAGCAAATATTAAAAAAGAACAAGTAGCACTTGAAGAAGCTGCTAAAAATATTGAAAAGAAAGTTAATGATATTTTAATCAGAATACCAAATATTTCAATGGAAGATGTGCCACTTGGTAAAGACGAAAACCAAAATAAAGTTATAAAAAATCATGATAAATTAGGACGTGGATTAGTTTCAGGCGTTCAACCTCACTATGTTATAGCTAAAGACTTAGACATTATCGATCTTGAAAGAGCAGTAAAAATGTCAGGTTCAAGATTTGTAATTTACAAAGGTTTAGGTGCTAGATTAGCTAGAGCTATTATGAATTTTATGTTAGATTTACATACCAAAAATGGTTATGAAGAATATTCGGTACCTGTAATAGTTAAACCTGAAATTCTTTTTGGTACAGGTCAATTGCCTAAATTTAAAGAAGATCTTTTTGCTTTAGAAGATGCTCAAAGTTATTTAATTCCAACAGCTGAAGTCCCTTTGACAAACTACTATAATAATGAAATAATAGATTTATCAAAACCTATTAGAATGACAGCATTTACTGAATGTTTTAGATCAGAAGCAGGAAGTAGTGGAAAAGATACAAGAGGTATTATTAGAAACCACCAATTCAAAAAAGTTGAATTAGTAAAAATTGTTAGTGAAAAAGATTGA", "aa": "MLNLKYVLENEKKVKEALKNRNFDLKQFNELIKEANKRGKLMFEAQSKKAELTKFSKEFAANKNNKTKLAKLQKEVANIKKEQVALEEAAKNIEKKVNDILIRIPNISMEDVPLGKDENQNKVIKNHDKLGRGLVSGVQPHYVIAKDLDIIDLERAVKMSGSRFVIYKGLGARLARAIMNFMLDLHTKNGYEEYSVPVIVKPEILFGTGQLPKFKEDLFALEDAQSYLIPTAEVPLTNYYNNEIIDLSKPIRMTAFTECFRSEAGSSGKDTRGIIRNHQFKKVELVKIVSEKD", "aa_hexdigest": "59c88f385cb38c1536d973d3b1ff70af", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [ "serS" ], "truncated": "3-prime", "pseudogene": { "start": 843854, "stop": 845038, "observations": { "mutation": [ 844733, 844868 ], "stop": [ 844733, 844868 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFU2", "query_cov": 1.0, "subject_cov": 0.6926713947990544, "identity": 1.0, "score": 526.0, "evalue": 4.9e-186, "gene_start": 1, "gene_end": 293, "reference_start": 1, "reference_end": 293, "reference_sequence": "MLNLKYVLENEKKVKEALKNRNFDLKQFNELIKEANKRGKLMFEAQSKKAELTKFSKEFAANKNNKTKLAKLQKEVANIKKEQVALEEAAKNIEKKVNDILIRIPNISMEDVPLGKDENQNKVIKNHDKLGRGLVSGVQPHYVIAKDLDIIDLERAVKMSGSRFVIYKGLGARLARAIMNFMLDLHTKNGYEEYSVPVIVKPEILFGTGQLPKFKEDLFALEDAQSYLIPTAEVPLTNYYNNEIIDLSKPIRMTAFTECFRSEAGSSGKDTRGIIRNHQFKKVELVKIVSEKDWEAEFNQMLEQAKSVLEELEIPYRELLLCTGDLGFSSRKTIDLEVWLPSEIKYREISSVSYMGDFQARRAMIRYKNENGENVYAHTMNGSGLAIDRLIAAILENYQNKDGSITIPKKLVPYMDGITKITK" }, "paralog": false, "identity": 0.9949238578680203, "score": 727.0, "evalue": 2.05e-267, "description": "Internal stop codon at 844733, 844868. Nonsense mutation around 844733, 844868.", "psc": { "uniref90_id": "UniRef90_C4XFU2", "gene": "serS", "product": "serine--tRNA ligase", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_Q6KIJ8", "kegg_orthology_id": "K01875", "go_ids": [ "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFU2", "EC:6.1.1.11", "UniRef:UniRef50_Q6KIJ8", "KEGG:K01875", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KIJ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KIJ8" ], "product": "Serine--tRNA ligase" } }, "id": "OAIKOHHJFB_1520", "locus": "GCA000209735_01513" }, { "type": "cds", "sequence": "contig_1", "start": 844754, "stop": 844870, "strand": "+", "frame": 2, "gene": null, "product": "Seryl-tRNA(Ser/Sec) synthetase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920HIT7" ], "nt": "ATGCTTGAACAAGCTAAATCGGTTTTAGAAGAATTAGAAATACCTTATAGAGAATTATTATTATGTACAGGTGACTTAGGATTCTCAAGTAGAAAAACTATTGACTTAGAAGTTTGA", "aa": "MLEQAKSVLEELEIPYRELLLCTGDLGFSSRKTIDLEV", "aa_hexdigest": "93f8c13faf2742f3e7b9ef82e59c43b7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A920HIT7", "query_cov": 1.0, "subject_cov": 0.8260869565217391, "identity": 0.579, "score": 48.9, "evalue": 6.19e-07, "valid": false, "product": "Seryl-tRNA(Ser/Sec) synthetase", "uniref50_id": "UniRef50_A0A920HIT7" }, "pscc": { "uniref50_id": "UniRef50_A0A920HIT7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920HIT7" ], "product": "Seryl-tRNA(Ser/Sec) synthetase" }, "genes": [], "id": "OAIKOHHJFB_1521", "locus": "GCA000209735_01514" }, { "type": "cds", "sequence": "contig_1", "start": 844871, "stop": 845125, "strand": "+", "frame": 2, "gene": null, "product": "aminoacyl--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B1930" ], "nt": "TTGCCTTCAGAAATTAAGTATCGTGAAATATCATCAGTTTCATATATGGGAGATTTCCAAGCACGTCGTGCAATGATTAGATATAAGAATGAAAATGGTGAAAATGTTTATGCTCATACCATGAATGGTTCAGGTCTTGCAATTGATAGATTAATTGCAGCAATTCTCGAAAATTATCAAAACAAAGATGGTTCAATTACTATACCTAAGAAATTAGTTCCTTATATGGACGGAATAACAAAAATTACAAAATAA", "aa": "MPSEIKYREISSVSYMGDFQARRAMIRYKNENGENVYAHTMNGSGLAIDRLIAAILENYQNKDGSITIPKKLVPYMDGITKITK", "aa_hexdigest": "5f51a468b16bf6f300185d105ab8bf5c", "start_type": "TTG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI00387B1930", "query_cov": 0.9404761904761905, "subject_cov": 1.0, "identity": 0.696, "score": 112.0, "evalue": 9.2e-31, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_UPI00387B1930", "kegg_orthology_id": "K01875" }, "pscc": { "uniref50_id": "UniRef50_UPI00387B1930", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387B1930" ], "product": "aminoacyl--tRNA ligase-related protein" }, "genes": [], "id": "OAIKOHHJFB_1522", "locus": "GCA000209735_01515" }, { "type": "cds", "sequence": "contig_1", "start": 845652, "stop": 846143, "strand": "-", "frame": 3, "gene": null, "product": "HpyAIV family type II restriction enzyme", "db_xrefs": [ "EC:3.1.21.4", "GO:0009036", "GO:0009307", "SO:0001217", "UniRef:UniRef50_C4XE10", "UniRef:UniRef90_C4XFU3" ], "nt": "ATGGAATACAATCAATTTGAAATGGAACTTAGAAGTGCTTTAACAACAGGCAATGGTACAAAACTTTTATACAAAATAATTGATGCACCTTATAGATTTTTTTCACCTTTGCAACCTTTTAATTACAAAGTTAAATTAGAACAAGCATTCTTAAGAACACAAGAAAACACATATGTAAAATTTATTAAATATTGTGCTGAATATTTAATTACTCAATATGGTTTTAAATTAGAAAAAAGTTCTTTTAAATATACTTTACCACTTAAAGAGGGAGAAGAATTCCCAGAAGAAGTAAAAGTCAATGCAGCTATGATTTTTAGACAAAAAGAAAATGGCGGAGATGAAGAAACAGCTGATATTTGTGTAATTTACTTGAAAAAAAGAGATTACTATGGAGCTAAAGAAGTTACTAAACTTCATGACAATATCATTAAGCAATTAGAAGTATTAAGAATAATTTATCCTAATAATAAAATCAAAGGTATCTTATGA", "aa": "MEYNQFEMELRSALTTGNGTKLLYKIIDAPYRFFSPLQPFNYKVKLEQAFLRTQENTYVKFIKYCAEYLITQYGFKLEKSSFKYTLPLKEGEEFPEEVKVNAAMIFRQKENGGDEETADICVIYLKKRDYYGAKEVTKLHDNIIKQLEVLRIIYPNNKIKGIL", "aa_hexdigest": "811897c888de565fa2d70d057c393c28", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 845349, "stop": 846149, "observations": { "mutation": [ 845390, 845516, 845654 ], "stop": [ 845390, 845516, 845654 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFU3", "query_cov": 1.0, "subject_cov": 0.5174603174603175, "identity": 1.0, "score": 323.0, "evalue": 1.25e-109, "gene_start": 1, "gene_end": 163, "reference_start": 3, "reference_end": 165, "reference_sequence": "MIMEYNQFEMELRSALTTGNGTKLLYKIIDAPYRFFSPLQPFNYKVKLEQAFLRTQENTYVKFIKYCAEYLITQYGFKLEKSSFKYTLPLKEGEEFPEEVKVNAAMIFRQKENGGDEETADICVIYLKKRDYYGAKEVTKLHDNIIKQLEVLRIIYPNNKIKGILWFLDNDYHKNVNFFNECYDKEHSFETPGFRAYYGAQFFKIFDKEEDWLLVENHIKKFKNSNYDYFLKMPDLDQDEDVLNAMINLNETSWQKLISPDEVYANIRQYIFNERNENSNLFKALKLRDIKRTSVDDDEEKRRILELENKANQNE" }, "paralog": false, "identity": 0.9849624060150376, "score": 533.0, "evalue": 6.34e-195, "description": "Internal stop codon at 845390, 845516, 845654. Nonsense mutation around 845390, 845516, 845654.", "psc": { "uniref90_id": "UniRef90_C4XFU3", "product": "HpyAIV family type II restriction enzyme", "ec_ids": [ "3.1.21.4" ], "uniref50_id": "UniRef50_C4XE10", "go_ids": [ "GO:0009036", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFU3", "EC:3.1.21.4", "UniRef:UniRef50_C4XE10", "GO:0009036", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_C4XE10", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE10" ] } }, "id": "OAIKOHHJFB_1523", "locus": "GCA000209735_01516" }, { "type": "cds", "sequence": "contig_1", "start": 846153, "stop": 846245, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATATTAAAATGAATCAATTCTTAGAATTTGATGAAGAAAAAACAAAAGAAGAAGATATTCTTCTTGAACTAGAACAAAACGTTGATTAG", "aa": "MNIKMNQFLEFDEEKTKEEDILLELEQNVD", "aa_hexdigest": "a270d6dae8cbc8a6f02061a52e16896c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3686.078700000001, "isoelectric_point": 4.09805736541748 }, "id": "OAIKOHHJFB_1524", "locus": "GCA000209735_01517" }, { "type": "cds", "sequence": "contig_1", "start": 846255, "stop": 846479, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAATTAGATTTACAATACAAAGCAAATTGTCACAAAATGCTCTTTTTAGTAGGTGAAAAGGACAAAATTATTGGTACTAAATATTTTGAAGATTATGCAATTAGTTTAGAAGTTCCAATTTTAATTATGGGTACGGGACACTCGCCAATTAAAGATGAACCAAAAAGAATTGCAAGATTTTTTAATGCATTGATTGAAAGCAAAAAAAGACACTTTTGA", "aa": "MEQLDLQYKANCHKMLFLVGEKDKIIGTKYFEDYAISLEVPILIMGTGHSPIKDEPKRIARFFNALIESKKRHF", "aa_hexdigest": "be5977e579ddc5690897837d38251f35", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8656.085799999993, "isoelectric_point": 8.761269569396973 }, "id": "OAIKOHHJFB_1525", "locus": "GCA000209735_01518" }, { "type": "cds", "sequence": "contig_1", "start": 846612, "stop": 846752, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGGTGGCGTGGCTACTGTTTTAGCTAAGGATCCTAAAGTTGTTAGATTAATTATGATGTCAACAATTAATCCTTATATGAAAGAATCTACTTCTTATTCAGTGTTACAATCTGTTATTAAACCTCAAAATAAATGA", "aa": "MAGGVATVLAKDPKVVRLIMMSTINPYMKESTSYSVLQSVIKPQNK", "aa_hexdigest": "9eb0c225c598cdb542dc8fee2cfafe2d", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5025.969600000002, "isoelectric_point": 9.873542594909669 }, "id": "OAIKOHHJFB_1526", "locus": "GCA000209735_01519" }, { "type": "cds", "sequence": "contig_1", "start": 846936, "stop": 847043, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAAACTATATTGAATACAATGGCGACAAAGTTCCTTTATATATAGAAGATAACAAAAAAAATACAACTTTTTTGTTTCTTCATGGACTTAATTCAAACTGA", "aa": "MKLNYIEYNGDKVPLYIEDNKKNTTFLFLHGLNSN", "aa_hexdigest": "2a8e932a89320bde0eb10530101fc8ca", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4147.662500000001, "isoelectric_point": 6.517181587219238 }, "id": "OAIKOHHJFB_1527", "locus": "GCA000209735_01520" }, { "type": "cds", "sequence": "contig_1", "start": 847110, "stop": 847412, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10035.14" ], "nt": "ATGTACCTTAACAAATTATTCCCTCGTTACAAAATTGTTCAAGTTAAAATTTACTCGCCTCACATGAGTGCTATTAGAGAAGCAATTATTAGTGATAATAAAACTGTTAATAGCTTTACTATCACTAAAGGCGTTGGAGGTTACTCAGGAAATAACACCAAAGTTCTTTCATCAATTACTCTTTACAATCAAGTGCCACGTCTTATTAAGAAAGTTCGGGCCGTTGATCAAAACTCGCTGATTACTATTAGTAATGTTGCTAGTGTTGATGGAAAAATATATCTTCCTGAAAACAAATTCTAA", "aa": "MYLNKLFPRYKIVQVKIYSPHMSAIREAIISDNKTVNSFTITKGVGGYSGNNTKVLSSITLYNQVPRLIKKVRAVDQNSLITISNVASVDGKIYLPENKF", "aa_hexdigest": "b27be12c95e6d88241986559dd2e78de", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10035.14", "name": "DUF2179", "length": 53, "aa_cov": 0.53, "hmm_cov": 1.0, "evalue": 6.68089719030601e-14, "score": 47.618621826171875, "start": 39, "stop": 91 } ], "seq_stats": { "molecular_weight": 11222.946299999989, "isoelectric_point": 9.99583911895752 }, "id": "OAIKOHHJFB_1528", "locus": "GCA000209735_01521" }, { "type": "cds", "sequence": "contig_1", "start": 847536, "stop": 847760, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGCTTTCTTCTTTGCAATTATTGCAATAATAGGAGGTAGTGCAGGAGTTACTGGAATTATTGGCGAATATATGGCTGTCGTTAAACAAAAAAACTTTGGAACAATTAATGGTTATATCAACTTAGTAATTATTATTGTTTCAGTTGTACTTGGTACATACTTACCAGGAAGTTTATTATTAAATGACATTAATGGTTTTAAAGCAGATTCAGTGACTTGA", "aa": "MLAFFFAIIAIIGGSAGVTGIIGEYMAVVKQKNFGTINGYINLVIIIVSVVLGTYLPGSLLLNDINGFKADSVT", "aa_hexdigest": "8e8453df4bfebabf6b9316a0efcba96b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7738.106599999996, "isoelectric_point": 5.875696754455566 }, "id": "OAIKOHHJFB_1529", "locus": "GCA000209735_01522" }, { "type": "cds", "sequence": "contig_1", "start": 848274, "stop": 848576, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAATAAATCACAAAATGATGAAAAATTAAATCAATCATCAGTCGAAGAACATAATGTAAATGAAGAACATACACAAAAACAAAAAGACAAAGAAGTGATGGACGAGGTTAATGCATTCGAAAGAGACGTTTTACTTAATAATGGTAGAGTCGAAGACAAGCATGTTGAAGAACATCATGAAATGAATTCAATTCATTTAGATGACGTTGTTAACAAAAAAAATACTCAATACAAATACACAAAAATGTCGAATTTTGTGTTGAAATTATCTCGTTTTTATGCTCCTATGCCTCTTTGA", "aa": "MPNKSQNDEKLNQSSVEEHNVNEEHTQKQKDKEVMDEVNAFERDVLLNNGRVEDKHVEEHHEMNSIHLDDVVNKKNTQYKYTKMSNFVLKLSRFYAPMPL", "aa_hexdigest": "add831090532a0044360f9a2be48465e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11884.080499999991, "isoelectric_point": 5.637370872497558 }, "id": "OAIKOHHJFB_1530", "locus": "GCA000209735_01523" }, { "type": "cds", "sequence": "contig_1", "start": 848913, "stop": 849032, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTAAAGAATATGATGCATCGAATATTAAAGTTTTAGAAGGATTAGAAGCCGTTAGAGTTAGACCTGGTATGTACATCGGTACAACAGGAATTAGAGGTCTTCACCACCTTATTTGA", "aa": "MSKEYDASNIKVLEGLEAVRVRPGMYIGTTGIRGLHHLI", "aa_hexdigest": "a62aa19c0147e26c6a8c113928cd8d31", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4326.009699999999, "isoelectric_point": 8.230243492126466 }, "id": "OAIKOHHJFB_1531", "locus": "GCA000209735_01524" }, { "type": "cds", "sequence": "contig_1", "start": 849063, "stop": 849308, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02518.31" ], "nt": "ATGGCAGGTTTTGCAAACAAAGTAGAAATCACGATTACAAAGGATGGTTACATTGATGTTATCGATAATGGTCGTGGTATTCCTACAGCAGTTCACCCTAAAACTGGTCTTTCAACAGTTGAAACAGTTTTAACGGTTTTACATGCTGGCGGTAAGTTTGACTCTGACTCATATAAAGTTTCTGGTGGACTTCACGGGGTTGGTGCTTCAGTAGTTAATGGACTTAGCGATGACTTTGAAGTTTGA", "aa": "MAGFANKVEITITKDGYIDVIDNGRGIPTAVHPKTGLSTVETVLTVLHAGGKFDSDSYKVSGGLHGVGASVVNGLSDDFEV", "aa_hexdigest": "cdea0663282a34fd64db0bcb153c438c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034D3B81C", "query_cov": 1.0, "subject_cov": 0.84375, "identity": 0.58, "score": 102.0, "evalue": 6e-27, "valid": false, "product": "ATP-binding protein", "uniref50_id": "UniRef50_Q706I4" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02518.31", "name": "HATPase_c", "length": 77, "aa_cov": 0.9135802469135802, "hmm_cov": 0.8181818181818182, "evalue": 1.4452827223397613e-11, "score": 40.88747787475586, "start": 4, "stop": 77 } ], "seq_stats": { "molecular_weight": 8347.249399999999, "isoelectric_point": 5.032149314880371 }, "id": "OAIKOHHJFB_1532", "locus": "GCA000209735_01525" }, { "type": "cds", "sequence": "contig_1", "start": 849969, "stop": 850673, "strand": "+", "frame": 3, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380DNC1" ], "nt": "ATGGCTGTTAACAAAGTGTTTTCAGATTCATTTGAACGTTTCTTAAATGAAAATCCTGCTGAAGCAAAAACTATTGTTCAAAAAACAATTTTTGCTAAAAAATCTCGTCTTGCTGGTTTAGCAGCTAGAGATGCAGCTAGAAGAAAAACTGTTTTTGATACAGGTTCACTTCCAGGAAAATTAGCTGACTGTTCTTCAAAAAATGCTGAAATTAGTGAGCTTTACATTGTCGAAGGTAACTCAGCTGGTGGTTCAGCTAAAATGGGAAGAAACAGAATGACTCAAGCTATCTTGCCTCTTCGTGGTAAAGTTATTAATGCTGAAAAGAATCAACCTAAAAAAGTGTTTGCAAATGCTGAAATTCTTTCATTAATTACAGCACTTGGAACAGGTGTCGGTGAAGAATTTAATATTAATAAATTAAGATATCACAAAATTATCATCATGACCGATGCCGATGTCGATGGTGCTCACATTAGAACATTGTTATTGACGTTCTTCTACCGTTACTTTAGACCTTTAATTGAATACGGATTTATTTATATTGCTCAACCTCCTTTATATAAAATAGCTCAAAACAAATTTATTGGATATGCTTACAACGATGCTCAAAAAGAAGAAATTATGCAAGGCTTAAATCAAAATCAAAAAGTAACAGTTCAACGTTACAAAGGACTTGGTGAAATGGACCCTGAACAACTTTGA", "aa": "MAVNKVFSDSFERFLNENPAEAKTIVQKTIFAKKSRLAGLAARDAARRKTVFDTGSLPGKLADCSSKNAEISELYIVEGNSAGGSAKMGRNRMTQAILPLRGKVINAEKNQPKKVFANAEILSLITALGTGVGEEFNINKLRYHKIIIMTDADVDGAHIRTLLLTFFYRYFRPLIEYGFIYIAQPPLYKIAQNKFIGYAYNDAQKEEIMQGLNQNQKVTVQRYKGLGEMDPEQL", "aa_hexdigest": "b0dd183ac76f8b59f866f16a6b0b59f4", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_A0A380DNC1", "query_cov": 0.9914529914529915, "subject_cov": 0.8083623693379791, "identity": 0.586, "score": 276.0, "evalue": 1.96e-90, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0A380DNC1" }, "pscc": { "uniref50_id": "UniRef50_A0A380DNC1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380DNC1" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "OAIKOHHJFB_1533", "locus": "GCA000209735_01526" }, { "type": "tRNA", "sequence": "contig_1", "start": 851180, "stop": 851253, "strand": "-", "gene": "trnG", "product": "tRNA-Gly(gcc)", "amino_acid": "Gly", "anti_codon": "gcc", "score": 66.5, "nt": "GCGAATGTAATTTAATGGTAAAATGCCAGCTTGCCATGCTGATAATGGGGGTTCGATTCCCCTCGTTCGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 851227, 851229 ], "id": "OAIKOHHJFB_19", "locus": "GCA000209735_01527" }, { "type": "ncRNA", "class": null, "sequence": "contig_1", "start": 851452, "stop": 851548, "strand": "-", "gene": "Bacteria_small_SRP", "product": "Bacterial small signal recognition particle RNA", "score": 46.4, "evalue": 1.4e-07, "db_xrefs": [ "RFAM:RF00169", "SO:0000655" ], "nt": "TTGTAGCGTCAGTAGAACAATAACCTTGTAACCTGGTCAGGTCAGAGATGAAGCAGCCATATCGAGTAGTGTTGTGTCTATTGACTTTTTTTATTTT", "id": "OAIKOHHJFB_43", "locus": "GCA000209735_01528" }, { "type": "cds", "sequence": "contig_1", "start": 851708, "stop": 851914, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00356.27" ], "nt": "ATGAAAAATTTCTCATATAAAAATATTGCTAAAGAAGCAGGTGTCTCTATTAGTACTGTAAGCCGTTACTATCGTGGTGGTTATGTTAGCAAGGAAACTAAAAAAGTTATTGCTAATATCATAAAAGAAAACGATTATTATCCAAATCATGGAGCCAGAACAATTCGTGGCCGTGACACTTCTATCTTTATTATTGTGCCTGAGTGA", "aa": "MKNFSYKNIAKEAGVSISTVSRYYRGGYVSKETKKVIANIIKENDYYPNHGARTIRGRDTSIFIIVPE", "aa_hexdigest": "ec7b210ac875650da08381d424306d54", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00356.27", "name": "LacI", "length": 44, "aa_cov": 0.6323529411764706, "hmm_cov": 1.0, "evalue": 4.444480437412897e-15, "score": 51.34090042114258, "start": 7, "stop": 49 } ], "seq_stats": { "molecular_weight": 7759.767100000002, "isoelectric_point": 9.791796684265137 }, "id": "OAIKOHHJFB_1534", "locus": "GCA000209735_01529" }, { "type": "cds", "sequence": "contig_1", "start": 851939, "stop": 852049, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGAATTGAACAAGGAGCAAAGACAAGAAATAGAAAAGTTGTTATAACTCACTCTTCACCTAATCCTGAAGAATATATTGAAACTATCAAATATGTTTCAAGCTGA", "aa": "MNGIEQGAKTRNRKVVITHSSPNPEEYIETIKYVSS", "aa_hexdigest": "cf9bdc96af1bf43440c5738923bdd480", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4077.5335000000014, "isoelectric_point": 8.161713600158691 }, "id": "OAIKOHHJFB_1535", "locus": "GCA000209735_01530" }, { "type": "cds", "sequence": "contig_1", "start": 852056, "stop": 852175, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAATAGTTTTCTTTCTTCCTGAAGATCCTCAAAACACAATTACAAACTATATTAAGAACACAAATTTTGAATCAAATATTTTAGTTTATGGAAAAGAAGTAGAAACATTAAATTGA", "aa": "MAIVFFLPEDPQNTITNYIKNTNFESNILVYGKEVETLN", "aa_hexdigest": "22f8da0404af7745050b8ab6046c161f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4521.060600000001, "isoelectric_point": 4.251295280456543 }, "id": "OAIKOHHJFB_1536", "locus": "GCA000209735_01531" }, { "type": "cds", "sequence": "contig_1", "start": 852693, "stop": 852947, "strand": "-", "frame": 3, "gene": "ssrA", "product": "SsrA RNA (TmRNA)-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P825" ], "nt": "ATGTTAGTTAAGTGTGATGAAACTAGAGATCGCAAATTATTGATGCATAAAAATGAAATAGTTAGATTGCAGTCTAAACTTCATAAGCTAGGTCATGCAACTATTATTCCAAGCAAAATTTATTTTGATAATCGCTCACGTATTAAGATTGAAATAGCTTTAGTTCAAGGAATGAAAAAAACAGATAAACGTGAAGAAATCAAAAAAAGAGATAATGAAAGATACATCAAAAAAGTCTTGAAAAATGTATATTAA", "aa": "MLVKCDETRDRKLLMHKNEIVRLQSKLHKLGHATIIPSKIYFDNRSRIKIEIALVQGMKKTDKREEIKKRDNERYIKKVLKNVY", "aa_hexdigest": "7f4e784c6ff2976e5520b178335a04d8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P825", "query_cov": 0.9642857142857143, "subject_cov": 0.8901098901098901, "identity": 0.506, "score": 81.3, "evalue": 1.95e-18, "valid": false, "product": "SsrA RNA (TmRNA)-binding protein", "uniref50_id": "UniRef50_A0A3B0P825", "kegg_orthology_id": "K03664" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P825", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P825" ], "product": "SsrA RNA (TmRNA)-binding protein" }, "genes": [ "ssrA" ], "id": "OAIKOHHJFB_1537", "locus": "GCA000209735_01532" }, { "type": "cds", "sequence": "contig_1", "start": 853274, "stop": 853393, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAATCTTCTTTAGAACAATTAGACAAATTAGACAACATTCCAGTTTATCGTGTAGATGTTGATGAAAATACAAAGCTAACTCAAGAAATGGGTGTTAATTCAATGCCAACATGA", "aa": "MFKSSLEQLDKLDNIPVYRVDVDENTKLTQEMGVNSMPT", "aa_hexdigest": "444ad36c6f5862ce8e726c07b9f6df7a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4487.048000000001, "isoelectric_point": 4.385492134094237 }, "id": "OAIKOHHJFB_1538", "locus": "GCA000209735_01533" }, { "type": "cds", "sequence": "contig_1", "start": 853409, "stop": 853498, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAAAGAAATTAATCAAGATGAATACAAGAACAATGTTATTGGAAAAGAAAAAGGCATTTATGTTTTGGTTTTTCACGCATTATGA", "aa": "MLKEINQDEYKNNVIGKEKGIYVLVFHAL", "aa_hexdigest": "fbeb342cc3e90903ea2cbb81877de32e", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3406.9455000000007, "isoelectric_point": 6.523149681091309 }, "id": "OAIKOHHJFB_1539", "locus": "GCA000209735_01534" }, { "type": "cds", "sequence": "contig_1", "start": 853802, "stop": 855844, "strand": "-", "frame": 1, "gene": null, "product": "Phase variable surface lipoprotein P78", "db_xrefs": [ "RefSeq:WP_041594252.1", "SO:0001217", "UniParc:UPI0002F591DE", "UniRef:UniRef100_C4XFV0", "UniRef:UniRef50_O06764", "UniRef:UniRef90_O06764" ], "nt": "ATGAAAAAAAGAAAATTTATATTTGCTTTATCATCATTAACAACAGCTTTTTTAGCTACACCTCTTATTTCTTCATCATGTACTAATGCTGATAAATTGTCTAATCTATTAGATGATTTAGACATTACTATTCCAGATTCAGAATCTAAAAAACCAAATGAAATCAAAGAATCGGATTTAAAATTTTACTCAAAAAATATGCCAAGTTTTAATAGTGAATATACTGTTGAAATACAAAAAATTCAAAAATATTCTTCAGAACTTGTAGTAACTTTTAGACTACGCGAAAAAAACTCAAACATTGTTTCAAAATACAAAACAATTACATACAAAAATTTCTTAAACTTTGATCATATTAATGAAGCTTTAACAGTACAATATTCTAATTCAAAAAATACTTATGTAAAAGATGTTGCTCAAACAAGTGACATTATCTTTAGAGATATTAAAACAGGATACAAAATTATCGATGTTAAAATTGATAACTCAAGAATTGAAGAATATCAAAAAAATTTATCTGACAAACTAAAAGATATAAATGAATTCTTAACAAAAAATAAATCTGAATATGATGCTGAAAAAGCTAAAGCAAATCCTAATAAGGAAATTATTGAAAAATATGAACGCGCTTTAATTAGAAAAGCTAAATATGAAGATACTGCGGATAAAGATTCATATATCAAATCATTCGAGAATTTAAATATAAAATACAAAATTCAATCAATAAATGATCCTAAAATTGTATCTGATGAATTCAGTCGTTCAATTCCAAACTTCAAAAATGATTCATACATTGATTACTATGTTTTTGATAAAAGTGACACATACTCAACAGTTTCGCATTCATTTCACTCTGTTTCAGATTTAACTGAAGAAATAAAGAAATACATTAAAAAAACTGCTTCACTAGAATATACAGGAAATACAAATGTTTCTGTATCTGAAGTTAAGGCTGATAAATTTAAATTTAATTTTGACACACCAATTGATTTTTCAATAGAAAACATCAAAGTTGAACCTGCTGATTCAGAAGGCAAAGTAAATCTTAAATTTAATATTAAAGCACCTATTAACAAACAAGCAACAACTTTATTAGTTGATGAAAACAAAAAAACAATTGAATTTGATTATGCATGTGAAATAGAATTTAACAAAAAAGATAGTAAAGAAAAATTAAGTGAAATTGAAAAACAAATTAGCTTTACATATGAAAATGCTGCAACAACAGAAGTTGATAAGGCAGAAGTTTCTAAAGTTAAATACACTTTATCTAACCCAAATTATGAAATTACTGATTTAAAAATTATTGGTTATGAATTCAACAATTCAACTATTGTTATTGAATACAAAGCAAAAAGTAAAAGCAGTTCAATTGAACAAGTTTATTCAAGAACAATTAGTGGCTTTTCTAAACTTTCACAATCTAAAATTGATGCAATTTTAGATTTAATTAAATTGAATTATAAACGTAAAAACTTAGTAGCTGCAAATGAAGCGAATAAAGATCATGTATTTATTGAATCAATGGATTCAAACTTCATGATTGAAGTTACAAAAATAGATTCATTTGATCTTAAAGATAAATCAATATTAGTTGAATTTAAATTATATGAAGTTGATGCACTTTCTAAATTAAAACCAGACAACAAAAAACGCGGTTTCAAATTTGTTAAATCATTCACAAAGAAAATTACAGGCTTCTCAGAAAAATCATTGACACAACATATCAATGAAGAAATTCAAAAGGTTACTTGTACTTGTCCAACAGCTTCAAGCAAATTGCCAAGTCAATTAAACATTAATGATATTAAATTTACTAACTTTGATAACAATATTTTTGAAATTGAAAAACCATCAATAATTGTTGCTAATGATCAAAAAGGCGAAGTTGTTATTACATTTAAATTACGTTACAAAATTGGTTATGTACAAAAAGTTTATGACCAACAAAAGAATGAATATGTTGAAAAAGAAATACCTTTCAAAACTGTTTCAGACTCAAAAACAGTTATATTAAATGGTTTTAAAAAAAATAAATTATAA", "aa": "MKKRKFIFALSSLTTAFLATPLISSSCTNADKLSNLLDDLDITIPDSESKKPNEIKESDLKFYSKNMPSFNSEYTVEIQKIQKYSSELVVTFRLREKNSNIVSKYKTITYKNFLNFDHINEALTVQYSNSKNTYVKDVAQTSDIIFRDIKTGYKIIDVKIDNSRIEEYQKNLSDKLKDINEFLTKNKSEYDAEKAKANPNKEIIEKYERALIRKAKYEDTADKDSYIKSFENLNIKYKIQSINDPKIVSDEFSRSIPNFKNDSYIDYYVFDKSDTYSTVSHSFHSVSDLTEEIKKYIKKTASLEYTGNTNVSVSEVKADKFKFNFDTPIDFSIENIKVEPADSEGKVNLKFNIKAPINKQATTLLVDENKKTIEFDYACEIEFNKKDSKEKLSEIEKQISFTYENAATTEVDKAEVSKVKYTLSNPNYEITDLKIIGYEFNNSTIVIEYKAKSKSSSIEQVYSRTISGFSKLSQSKIDAILDLIKLNYKRKNLVAANEANKDHVFIESMDSNFMIEVTKIDSFDLKDKSILVEFKLYEVDALSKLKPDNKKRGFKFVKSFTKKITGFSEKSLTQHINEEIQKVTCTCPTASSKLPSQLNINDIKFTNFDNNIFEIEKPSIIVANDQKGEVVITFKLRYKIGYVQKVYDQQKNEYVEKEIPFKTVSDSKTVILNGFKKNKL", "aa_hexdigest": "e2a8b7fa58f86d94940b8308f695385b", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0002F591DE", "ncbi_nrp_id": "WP_041594252.1", "uniref100_id": "UniRef100_C4XFV0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002F591DE", "RefSeq:WP_041594252.1", "UniRef:UniRef100_C4XFV0" ] }, "ips": { "uniref100_id": "UniRef100_C4XFV0", "uniref90_id": "UniRef90_O06764" }, "psc": { "uniref90_id": "UniRef90_O06764", "product": "Phase variable surface lipoprotein P78", "uniref50_id": "UniRef50_O06764" }, "pscc": { "uniref50_id": "UniRef50_O06764", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O06764" ], "product": "Phase variable surface lipoprotein P78" }, "genes": [], "id": "OAIKOHHJFB_1540", "locus": "GCA000209735_01535" }, { "type": "cds", "sequence": "contig_1", "start": 855863, "stop": 856003, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGGTGTTTCAAGATTCGCTGAACTACTCTACACATTGCCATTTATTATCACACTTGGAATTATGATTGCTAAAATGAAAAGCAAGCAAATGGGGCCAGCTGCTGCAGGTCGACCTTATGACAAATCAGAGCGTTAG", "aa": "MKGVSRFAELLYTLPFIITLGIMIAKMKSKQMGPAAAGRPYDKSER", "aa_hexdigest": "dd7652daf9c1160972968470b421fec8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5117.128300000002, "isoelectric_point": 10.11536350250244 }, "id": "OAIKOHHJFB_1541", "locus": "GCA000209735_01536" }, { "type": "cds", "sequence": "contig_1", "start": 856397, "stop": 856627, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTACTTTGCTTTTGCAGGTATCTTATCCTCGGGATTTGCACTGTTGTTTGGTTTCGCAACGATTAAACTTAAATCAGATCAATCTGTTTCAGGTTTCGCTATGAACATTCTTGCAGTTGGTGTTGCTTCTATCCTTTTATTATTTGTTAAAAAAGCACAACAAGGTGGAGACTACTTCCAAATGCGTGGCCAAACAGAACTTGCTTTAAGTCCTAATGTTGACAACTGA", "aa": "MYFAFAGILSSGFALLFGFATIKLKSDQSVSGFAMNILAVGVASILLLFVKKAQQGGDYFQMRGQTELALSPNVDN", "aa_hexdigest": "71a4c945dcbd207a07abe44b623c494e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8126.381999999997, "isoelectric_point": 8.098147773742678 }, "id": "OAIKOHHJFB_1542", "locus": "GCA000209735_01537" }, { "type": "cds", "sequence": "contig_1", "start": 856637, "stop": 856804, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTCAGCTCTAACACTTGCAACCCTTTCAGGTATTTTCTCAGAAAGAGTTGGTATTACAAACATTGCTGTTAACGGGTTTATGATTTTTGGAGCTGCTATGTATGCTCTAATAAGCTTCCTAATGACTGATGTTGTTTTCAAAGGTAAAACATTAAGTCCATGA", "aa": "MFSALTLATLSGIFSERVGITNIAVNGFMIFGAAMYALISFLMTDVVFKGKTLSP", "aa_hexdigest": "e07f8485bfe935da0a934d138da5976c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5864.954900000001, "isoelectric_point": 8.253774452209473 }, "id": "OAIKOHHJFB_1543", "locus": "GCA000209735_01538" }, { "type": "cds", "sequence": "contig_1", "start": 856918, "stop": 857085, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTATCTATGACGAAATGTCTAAGAAAAAATTCGAATATCTCAAAAAATACAGTGATTTTGGCTTAAACAATCTTAAAACATTAAAAAATCAATATAAAGCTGAAAAACACGAAAGAAAAATTCAATATATTACTAAAAGAGATTTAATTTATACATCTCATTGA", "aa": "MSIYDEMSKKKFEYLKKYSDFGLNNLKTLKNQYKAEKHERKIQYITKRDLIYTSH", "aa_hexdigest": "f2d3fd8a02e7960c808e320debbeaf2d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6808.792300000001, "isoelectric_point": 9.619150352478027 }, "id": "OAIKOHHJFB_1544", "locus": "GCA000209735_01539" }, { "type": "cds", "sequence": "contig_1", "start": 857134, "stop": 857250, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACCAAAAATGCTAAATATAGAGAATATGTTAAAGCTCTTAATGAAGAAAAAAGAAGGAATAGAAAACTCAAAAAAGAATTTGCTTCAATTAAAGAAGAATATCAAAAATGA", "aa": "MKTKNAKYREYVKALNEEKRRNRKLKKEFASIKEEYQK", "aa_hexdigest": "a34dee78871f7ca0cfabb5913749dc8f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4777.5102000000015, "isoelectric_point": 10.060952186584473 }, "id": "OAIKOHHJFB_1545", "locus": "GCA000209735_01540" }, { "type": "cds", "sequence": "contig_1", "start": 857305, "stop": 857445, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTATACAACAAGAGATTTATTTGCTATAAAAGATAAATTAACAAAAGACTTTTTCCCTATTATTACTGGTATAATTGTCTTTATGGCTGCTTTAGCTATTATGCTTTATAAATTCAAACCACTTGGTTTCATGTGA", "aa": "MIYTTRDLFAIKDKLTKDFFPIITGIIVFMAALAIMLYKFKPLGFM", "aa_hexdigest": "94e0e6c73b4c18eca007b21badbcf0dc", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5348.580299999999, "isoelectric_point": 9.601614952087402 }, "id": "OAIKOHHJFB_1546", "locus": "GCA000209735_01541" }, { "type": "cds", "sequence": "contig_1", "start": 857560, "stop": 857862, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAATCCAGAATCATATTCACCTACAACTATAGGTAGTGCTAGAATTGCAATTGAAAATGCAGTTAAATATCAATTTGTTTACTTTGGTATTGGAATGTTTGTTTTAACAGCTATTGTTTTATTTGTGATCTACAACTTCACAACATTAGGTTACAAAATAAACATGGTTGGTAAAAATCCTTCAAACGCTAAATATATTGGTCTTAACGAAAAAATGTTAACCATTATAGTTATGGGATTTTCAGGAGCTTTAGCTGGTTTCGCTGGATTCTACTACATTATTATCAAAGAAGGCTGA", "aa": "MFNPESYSPTTIGSARIAIENAVKYQFVYFGIGMFVLTAIVLFVIYNFTTLGYKINMVGKNPSNAKYIGLNEKMLTIIVMGFSGALAGFAGFYYIIIKEG", "aa_hexdigest": "118af7f5e609274ee04915dd610c8ecf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11045.922399999992, "isoelectric_point": 9.222927665710447 }, "id": "OAIKOHHJFB_1547", "locus": "GCA000209735_01542" }, { "type": "cds", "sequence": "contig_1", "start": 858452, "stop": 858736, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTCACCACACGACTTTATATTAATTCTTCAACCAACACGTGTACTTGACGTTGGGGCTATTAAAAATATCCATGAAAGAATTCTTAAAGAAAAAGCAGAAGGAAAAGCAATTCTTTTAATTTCTTATGAATTAGACGAGGTTCTTTCACTAGCTGATACTATTGCTGTTATTAACGAAGGCGAAATTAGACTTTCAAAACCCGCAAGAGAATTAACTAGAAAAGAAATCGGTCTTTACATGGCTCAAAAAGAACATCAAACGGAAGGAGGCACACAATAA", "aa": "MNSPHDFILILQPTRVLDVGAIKNIHERILKEKAEGKAILLISYELDEVLSLADTIAVINEGEIRLSKPARELTRKEIGLYMAQKEHQTEGGTQ", "aa_hexdigest": "5e7f5f6410a00877cbd8ee00f6036f23", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10563.08439999999, "isoelectric_point": 5.577576255798339 }, "id": "OAIKOHHJFB_1548", "locus": "GCA000209735_01543" }, { "type": "cds", "sequence": "contig_1", "start": 858899, "stop": 859093, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAGTAAAAATTATTAAATTCTTTAAAACCAAAAAAGATGAAGAGAAACTTAAAGACAGGATTAATTTAAACAATTATTCAGTCTTAGAAAAATCAATGCTTGCTTCAAGTTTTATTCCAAGTGATAGACATAAACATGGTCTAGCATTAGATTACAATATTAAAAATAATACAATATTAAGACGTTTATGA", "aa": "MIVKIIKFFKTKKDEEKLKDRINLNNYSVLEKSMLASSFIPSDRHKHGLALDYNIKNNTILRRL", "aa_hexdigest": "a620a6519f26e53850423a242e50ae21", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7590.869299999998, "isoelectric_point": 9.994485282897951 }, "id": "OAIKOHHJFB_1549", "locus": "GCA000209735_01544" }, { "type": "cds", "sequence": "contig_1", "start": 859151, "stop": 860254, "strand": "-", "frame": 1, "gene": "mglA", "product": "Galactose/methyl galactoside import ATP-binding protein MglA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M8X7" ], "nt": "ATGAACGCAATTGAATTTATTAATATTTCGAAAAGTTTCGGTGTAATCAAAGCTAACCAAGATATTAATATTCAAATTAAAAAAGGTACAATTCATGCACTTATTGGTGAAAATGGTGCAGGTAAAAGTACCTTGCTATCAATTCTTTTTGGACTATATGAACCCGACCATGGAATTATCAAGGTTAATGGTAAAGAGGTTTTAATAAATAATCCAAATGATGCTAATAATTTGGGTATTGGTATGGTTCACCAACATTTTAAATTGGTTGGTGCATACACAAACTTACAAAATATAATTCTTGGTGCTGAAAGTGAATCAAAAACTTTTAAAACTTTGGATTATAAACCATCAATTAAAAAAATTAAAACTATTCAAAGATTATTTAATTTAAATTTTGATTTGAATAAAGACACAGGCACTGAATCAGTTTCAACACAGCAAAAAGTTGAAATCATGAAGATGTTATATCGTGATTCAGAAATTCTAATCTTTGATGAACCAACTGCTGTTTTAACAGATGAAGAAATTCAAGGTCTTCTTGAAACATTCAAATTGTTTAAATCGCAAGGTAAAACAATCATTTTTATCTCTCACAAACTAAAAGAAGTTAAAGCTGTAGCTGATTATGCAACAGTTATACGTCACGGTAAAGTTACTGGTAACTTCGAAGTTGCTAATACTTCTATTGAAGAAATGGCTAACAGAATGGTTGGTGACAATATTGTTATGGCACAAAACCAATCACTTGACGTTTTTGAAGATAAAGACACAGTTTTAGAACTTGAAAATGTATCAACAAAAGGTACAAAATCACTTAAAAACTTAAATCTTAAGGTTCATGCTGGAGAAATTCTAGCTATTGCTGGTGTTGAAGGCAATGGACAACAAGATTTAGAATATGTTGTTAGTGGAATTGTTAAACCTACAAAAGGTAATGTCTACTTAAAGAAAACACCTTTAGTTGAGGAAAGAGTTAAATTCATTCATAAAAATAAAATCAAGAATTTCATTATTTTAAGTTCATTGCTTGCATTATTTATAGCTTTTGGATCAATTTTTTTTGGTGTTAGTCCATTTCAGCAAAATTATTCAAACATTTGA", "aa": "MNAIEFINISKSFGVIKANQDINIQIKKGTIHALIGENGAGKSTLLSILFGLYEPDHGIIKVNGKEVLINNPNDANNLGIGMVHQHFKLVGAYTNLQNIILGAESESKTFKTLDYKPSIKKIKTIQRLFNLNFDLNKDTGTESVSTQQKVEIMKMLYRDSEILIFDEPTAVLTDEEIQGLLETFKLFKSQGKTIIFISHKLKEVKAVADYATVIRHGKVTGNFEVANTSIEEMANRMVGDNIVMAQNQSLDVFEDKDTVLELENVSTKGTKSLKNLNLKVHAGEILAIAGVEGNGQQDLEYVVSGIVKPTKGNVYLKKTPLVEERVKFIHKNKIKNFIILSSLLALFIAFGSIFFGVSPFQQNYSNI", "aa_hexdigest": "e108c33640f696e8ee45810ad4a72326", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI00111B63FE", "query_cov": 0.9700272479564033, "subject_cov": 0.9246753246753247, "identity": 0.64, "score": 436.0, "evalue": 1.72e-149, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_A0A223M8X7" }, "pscc": { "uniref50_id": "UniRef50_A0A223M8X7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M8X7" ], "product": "Galactose/methyl galactoside import ATP-binding protein MglA" }, "genes": [ "mglA" ], "id": "OAIKOHHJFB_1550", "locus": "GCA000209735_01545" }, { "type": "cds", "sequence": "contig_1", "start": 862208, "stop": 862558, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGGGCATTAGCCCTCCCTAAAATATTTGGCGACTTGTCGCCCTTTCTAAAATATTATTTTAATTTTCCTATTTTTTTGAAAAACAAATTTCTATTCTTGTTACTTTCTCAGTTTCTCTTTTGTTTTCCATATAATAATTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCGTACATTTCTAAAGCAGATAAATATCCAAAAATTTCTCTTGGACGATTGTTTATTTCATTTTGTACTTTTAGAATTTCTTCTTCAGTAATATCATCAAAATTAGTTTTTTTCTTAAAAAATCTTCTAACTAAACCATTGAAATTTTCATTTAAACCCCTTTGA", "aa": "MRALALPKIFGDLSPFLKYYFNFPIFLKNKFLFLLLSQFLFCFPYNNLSIPVEFQGFSAFNFSYISKADKYPKISLGRLFISFCTFRISSSVISSKLVFFLKNLLTKPLKFSFKPL", "aa_hexdigest": "a34e56f8fcbc4f5181b851514f9999e3", "start_type": "GTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13575.125699999975, "isoelectric_point": 10.064046669006348 }, "id": "OAIKOHHJFB_1551", "locus": "GCA000209735_01546" }, { "type": "cds", "sequence": "contig_1", "start": 862704, "stop": 862838, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAAGAATTACCAGAAGATTTCGTTAAATATATTAATAGTGACAAAGCTTTAAAAGATGGTAATAAAATTGACAATGTTAGTGAAAGATTAGAAGCAATTATTTCTGCTATTAACAAGGCAGCAAAATAA", "aa": "MFKELPEDFVKYINSDKALKDGNKIDNVSERLEAIISAINKAAK", "aa_hexdigest": "e7960324786cd46f128a8fa539f7add7", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4967.6506, "isoelectric_point": 6.125163078308105 }, "id": "OAIKOHHJFB_1552", "locus": "GCA000209735_01547" }, { "type": "cds", "sequence": "contig_1", "start": 862947, "stop": 863249, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00217FF484", "UniRef:UniRef90_UPI00217FF484" ], "nt": "ATGAACACTGTTATTAATAATGTTTTATCTTCAACACCAGCTGATGTTAAATACAACCCACATGTTATCTTATCTGTTGCTGGACCTGCTACATTTGAAACTGTAAGATTAGCAAACAAAGGTCAATATGTAATTGGTGTTGACTCAGACCAAGGTATGATTCAAGACAAAGACAGAATTCTTACATCAGTTCTAAAACACATTAAACAAGCTGTTTATGAAACATTATTAGATCTTATTCTTGAAAAAGAAGAAGGATATAAACCATATGTAGTTAAAGACAAAAAAGCAGACAAAAAATGA", "aa": "MNTVINNVLSSTPADVKYNPHVILSVAGPATFETVRLANKGQYVIGVDSDQGMIQDKDRILTSVLKHIKQAVYETLLDLILEKEEGYKPYVVKDKKADKK", "aa_hexdigest": "ae68c9deea6e7b6e28f31007589be251", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 862704, "stop": 863447, "observations": { "mutation": [ 862919, 862949, 863438 ], "stop": [ 862919, 862949, 863438 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00217FF484", "query_cov": 1.01, "subject_cov": 0.40725806451612906, "identity": 0.99, "score": 190.0, "evalue": 2.76e-59, "gene_start": 1, "gene_end": 100, "reference_start": 67, "reference_end": 167, "reference_sequence": "MASWLSEQDESKRVVASFGGGAFPGVTTFNEGFAKGILYYNQKHKSSKIYHTSPVKLDSGFTAGEKMNTVINNVLSSTPADVKYNPHVILSVAGPATFETVRLAANKGQYVIGVDSDQGMIQDKDRILTSVLKHIKQAVYETLLDLILEKEEGYKPYVVKDKKADKKWSHFGTQKEKWIGVAENHFSNTEEQAKINNKIKEAIKMFKELPEDFVKYINSDKALKDGNKIDNVSERLEAIISAINKAAK" }, "paralog": false, "identity": 0.9758064516129032, "score": 466.0, "evalue": 2.09e-170, "description": "Internal stop codon at 862919, 862949, 863438. Nonsense mutation around 862919, 862949, 863438.", "psc": { "uniref90_id": "UniRef90_UPI00217FF484", "uniref50_id": "UniRef50_UPI00217FF484", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00217FF484", "UniRef:UniRef50_UPI00217FF484" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00217FF484", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00217FF484" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11159.751899999994, "isoelectric_point": 7.90738582611084 }, "id": "OAIKOHHJFB_1553", "locus": "GCA000209735_01548" }, { "type": "cds", "sequence": "contig_1", "start": 863628, "stop": 863990, "strand": "-", "frame": 3, "gene": null, "product": "Monocytic differentiation/activation factor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P94932", "UniRef:UniRef90_P94932" ], "nt": "ATGAAAAAGTCAAAAAAAATTTTATTAGGATTGAGTCCTATTGCTGCTGTTCTTCCTGCAGTAGCTGTTTCTTGTGGAAACAACGATGAATCCAATATTTCATTCAAAGAGAAAGATATTAGTAAATATACCACAACAAATGCTAATGGAAAACAAGTTGTTAAAAACGCTGAATTGTTAAAATTGAAACCAGTTCTTATTACAGATGAAGGTAAAATTGATGATAAATCATTTAACCAATCAGCTTTTGAAGCTTTAAAAGCTATAAATAAACAAACTGGTATTGAAATTAACAGTGTTGAACCTAGCTCAAACTTTGAAAGTACTTACAACAGTGCACTTTCAGCCGGACACAAAATTTGA", "aa": "MKKSKKILLGLSPIAAVLPAVAVSCGNNDESNISFKEKDISKYTTTNANGKQVVKNAELLKLKPVLITDEGKIDDKSFNQSAFEALKAINKQTGIEINSVEPSSNFESTYNSALSAGHKI", "aa_hexdigest": "d281e21809709d9d95038c579a47f68c", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 863432, "stop": 863990, "observations": { "insertion": [ 863648 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P94932", "query_cov": 1.0, "subject_cov": 0.6486486486486487, "identity": 0.9420000000000001, "score": 212.0, "evalue": 2.67e-68, "gene_start": 1, "gene_end": 120, "reference_start": 1, "reference_end": 120, "reference_sequence": "MKKSKKILLGLSPIAAVLPAVAVSCGNNDESNISFKEKDISKYTTTNANGKQVVKNAELLKLKPVLITDEGKIDDKSFNQSAFEALKAINKQTGIEINSVEPISNFESAYNSALQPDTKFEYLMASNTNNLLNNTLMLTEKNLKEIKSKSLVSTLILKQSTSDSTHYNSILKNLHLQQAMQLQVD" }, "paralog": false, "identity": 0.9891891891891892, "score": 346.0, "evalue": 1.47e-123, "description": "Frameshift due to insertion around 863648.", "psc": { "uniref90_id": "UniRef90_P94932", "product": "Monocytic differentiation/activation factor", "uniref50_id": "UniRef50_P94932", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P94932", "UniRef:UniRef50_P94932" ] }, "pscc": { "uniref50_id": "UniRef50_P94932", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P94932" ], "product": "Monocytic differentiation/activation factor" } }, "id": "OAIKOHHJFB_1554", "locus": "GCA000209735_01549" }, { "type": "cds", "sequence": "contig_1", "start": 864524, "stop": 865930, "strand": "-", "frame": 1, "gene": null, "product": "Putative hydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZ84", "UniRef:UniRef90_C4XFV7" ], "nt": "ATGAAAATTCCTAACTTAACAATTTATACTTCAGCATTCAACAAAATCATGATCATGGATCGTTTATCAAAATATAAGATTCCAACAAAAAACTACAAAGTTATGGTTATTAATAAAGTTACACCATTTAGTGACAAACTTTTTGTTAAGCCAATCGATTTAGCTGGTTCAATGCCAGGACACATTGGTTTTGACTTCATCACACCAGATGGAGATATCTTATTTATGTTTAACTTTGTCGAAGGTGACTTAGGAATTTATGGTAAATTAAGCTTTAATGAACTTAAACAAAGATTTACAAAACGTAAAATTTTAGCTCTAGTTGTTGACTCAGGTAAAGCAAACTTTGGTGGTAAGGCTATTGAAAAAATTGGTTTACCAGAAGGTATTAGAGATGTTTTTTTAGAAACCAAAAAGAATGAAAGAATAATAGTTGGTGCTTATGACGAAGAAATGGTAGCTATCCACAAAATTTTAGAATTAGCTCAAGAAACAAGTCGTCCAGTTGTAACTTATGGTAAAACTTATGGCCAAATATTTTATTTAATTAAAAAAGCTCATCCTGAACTTAAGTTACCAGAATTAATTGATTATCGTAATGCTAACAAAGTTAAAAATGCTGTTATTTTAGTAACTGGTGCTACTGAAAGACTTTACTCAAGATTTATTAGAATAACTGACAATAATGATGTTTTTTTAAAACTTCAAAAAAGCGATACTGTTGTAATGATTGCTCCTCCTATTAATGGACTTGAAAGTCTTGAAGCTTTAACTTTAGATGAAATTGCAAGAATTACTCCAAAAATTAGTGATGTTAATGCTAATGAATTTTATAGATGTCGTCCTGCTAAACAAGACTTAATTGATTTAGTTAATGCTCTAAATCCTGAATATGTGATTCCTGTGCAAGGACTTTATCGTTACTTAAATGAAGCTACTATAGCTATAAGTGAAAATACACAAGTGAAACAAAATCATTGTTTAGTTATGCAAAATGGAAAAATTGTTCACTTTATTGATGGCAAAATGTCAACAACCAAGGGCAAAGTTAAAGAAATTGGCGACACTATTATTGATGGTTTTGGTGTTGGCGATATTAGTACTGAGGTTATTAGTGAGCGTGAAGCTTTAGGTCGTGAAGGTGTGATTTTAGTGTCTTCTCAATATAATCCAAAAACAAAATTATTAACTGGTAAATTACAAATAAATTTTGTTGGTGTCATAAACAAAGAGGAAAAAAATGAAGCTAGCGACATAATCAAAAGCACAATTGTAAAATTAATTGAAACAGAAAGTTTTAATGGAATAAAAGATTTCCAAAATGAAGCTCGTCATGCTATTAGAAAAAGAATTTACAAAACTTTTGAAAAAGAACCAATAGTAATTGTAACATTGGCTCAAATTTAA", "aa": "MKIPNLTIYTSAFNKIMIMDRLSKYKIPTKNYKVMVINKVTPFSDKLFVKPIDLAGSMPGHIGFDFITPDGDILFMFNFVEGDLGIYGKLSFNELKQRFTKRKILALVVDSGKANFGGKAIEKIGLPEGIRDVFLETKKNERIIVGAYDEEMVAIHKILELAQETSRPVVTYGKTYGQIFYLIKKAHPELKLPELIDYRNANKVKNAVILVTGATERLYSRFIRITDNNDVFLKLQKSDTVVMIAPPINGLESLEALTLDEIARITPKISDVNANEFYRCRPAKQDLIDLVNALNPEYVIPVQGLYRYLNEATIAISENTQVKQNHCLVMQNGKIVHFIDGKMSTTKGKVKEIGDTIIDGFGVGDISTEVISEREALGREGVILVSSQYNPKTKLLTGKLQINFVGVINKEEKNEASDIIKSTIVKLIETESFNGIKDFQNEARHAIRKRIYKTFEKEPIVIVTLAQI", "aa_hexdigest": "b3d877f488c310ec82246759f5e90521", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XFV7", "query_cov": 1.0, "subject_cov": 0.8387096774193549, "identity": 1.0, "score": 915.0, "evalue": 0.0, "valid": true, "uniref50_id": "UniRef50_A0A448ZZ84" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZ84", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZ84" ], "product": "Putative hydrolase" }, "genes": [], "id": "OAIKOHHJFB_1555", "locus": "GCA000209735_01550" }, { "type": "cds", "sequence": "contig_1", "start": 865937, "stop": 866176, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002201F3D4" ], "nt": "ATGAATCATATTAATATTTTTGCTCTTGGTGGGCTTGATGAAAATGGTAAAAACTGTTATGTTTTCGAATTCAATGATGACATTTACATTATTAACTCAGGAACTAAAATTCCAATCAACTCAAATAATGGAGTTGATACTTTAATTCCTTCTTTTGAATACTTAGAAAAACACAAAGATAGAATTAAAGGTATCTTTATTTCTGATGTTAAAAATGAATCGTTTAGTGCATTGCCTTGA", "aa": "MNHINIFALGGLDENGKNCYVFEFNDDIYIINSGTKIPINSNNGVDTLIPSFEYLEKHKDRIKGIFISDVKNESFSALP", "aa_hexdigest": "cf88b56f74275f1fbf1d11bd63f36936", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI002201F3D4", "query_cov": 0.9746835443037974, "subject_cov": 0.875, "identity": 0.636, "score": 108.0, "evalue": 1.61e-29, "valid": false, "uniref50_id": "UniRef50_UPI002201F3D4" }, "pscc": { "uniref50_id": "UniRef50_UPI002201F3D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002201F3D4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8909.930499999995, "isoelectric_point": 4.8840837478637695 }, "id": "OAIKOHHJFB_1556", "locus": "GCA000209735_01551" }, { "type": "cds", "sequence": "contig_1", "start": 866255, "stop": 866419, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAGAGAGCTTACTAAAATACATGAAGAATTTATTTATGCAACCGCAACTGAACTATTAGCAAAAAACAAAACTAGTCCTTTTAAAGGTGAGATAACTTTAGTTATCAAACTAAAAGAAATTAAAAAAGAGAAAATAAATAAATATGCTCACTTAAAATAA", "aa": "MARELTKIHEEFIYATATELLAKNKTSPFKGEITLVIKLKEIKKEKINKYAHLK", "aa_hexdigest": "11e25ee0c2d353e13d5e9c128a343d0d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6301.4431, "isoelectric_point": 9.647580909729005 }, "id": "OAIKOHHJFB_1557", "locus": "GCA000209735_01552" }, { "type": "cds", "sequence": "contig_1", "start": 866441, "stop": 866965, "strand": "-", "frame": 1, "gene": null, "product": "Ribosomal RNA small subunit methyltransferase I", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N7" ], "nt": "ATGCCTAAAATATATATTGTTGGAACACCTATTGGAAACTTAAAAGATATAACTTTAAGAGCTCTTGAAACTCTTAAGGCTGTTGATGTTATTGCTTGCGAAGATACTCGTGTTAGTGCTAAATTATTGAACCATTATGACATTAAAAAACGTTTAATTTCATACAACAAAATCAATGAAAAAGCAAGTGCCCAAGGCCTTATTGATTTAATTGAAAAAGATGACTTAAAAATGGCTTTAATTAGTGATGCAGGTATGCCTTTAGTTAGTGATCCGGGTTTTGAATTAATTAAACAAGCAAGAGAAAAAAATATTGAATTAGAACTAATTCCAGGAGTCAATGCAGCTATTAGTGCTTTTGCTTTTAGTGGACTAAGTAATACCTTTATTTTTATGACTTTTCCTAAAGAAAAAAGTGGCCAAAGATTAGAACAAGTCAAAAGCTTCACAAGTGAACATGCATATGTTTTTTATGTAGCTCCGCATAAATTAGAAAGTTTCTTAAAAGACATTGACTCAGTGTGA", "aa": "MPKIYIVGTPIGNLKDITLRALETLKAVDVIACEDTRVSAKLLNHYDIKKRLISYNKINEKASAQGLIDLIEKDDLKMALISDAGMPLVSDPGFELIKQAREKNIELELIPGVNAAISAFAFSGLSNTFIFMTFPKEKSGQRLEQVKSFTSEHAYVFYVAPHKLESFLKDIDSV", "aa_hexdigest": "620f13f2f9abb04a43e6141dc9760c69", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0010050DA8", "query_cov": 1.0344827586206897, "subject_cov": 0.8181818181818182, "identity": 0.5, "score": 168.0, "evalue": 9.39e-50, "valid": false, "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "ec_ids": [ "2.1.1.198" ], "uniref50_id": "UniRef50_D1J7N7", "kegg_orthology_id": "K07056", "go_ids": [ "GO:0000451", "GO:0008649", "GO:0008757" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7N7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N7" ], "product": "Ribosomal RNA small subunit methyltransferase I" }, "genes": [], "id": "OAIKOHHJFB_1558", "locus": "GCA000209735_01553" }, { "type": "cds", "sequence": "contig_1", "start": 867357, "stop": 867875, "strand": "-", "frame": 3, "gene": null, "product": "DNA polymerase III subunit delta", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFV9", "UniRef:UniRef90_C4XFV9" ], "nt": "ATGGTTTCAAAAAATGTCATAACTATTTTAGAAAACTCAATAAAAAATAACAAACTTAGTCACTGTTATTTACTAAAAGCTCCTTCAGGTTTAAACATCGATGATTCAATTTTATACATGATTAATAAACTTTGCAACTCACAATTGCAATCTTTAGATCTTGAAACAATGCTGCCTAATGTTCAAATTTTTGAAGATAATGGCACTGAAAATAATCTTAAAAAAGATAAAATTGTGGCCGGCTTTGAAGAATCAAGTTTATCTTCATTTGTTGAAGGGCAAAAGAAGATAACTGTATTTAAAAATGTTGAAAATGCTTCTAAAGCTGCTCTTAATTCATTACTTAAAATAATTGAAGAACCAAGTGATAATGTAGTCTTTATTTTAACAACTAATAACATAACAAAAGTTTTAGAAACCATCAAATCAAGATCAATAACAATTAACATTAATGCGCCTTCAAACGAAGAGTTAGCAGAAGAATTAAAAGGTGAAAATTTAAAAGAAGAAGCTATATGA", "aa": "MVSKNVITILENSIKNNKLSHCYLLKAPSGLNIDDSILYMINKLCNSQLQSLDLETMLPNVQIFEDNGTENNLKKDKIVAGFEESSLSSFVEGQKKITVFKNVENASKAALNSLLKIIEEPSDNVVFILTTNNITKVLETIKSRSITININAPSNEELAEELKGENLKEEAI", "aa_hexdigest": "b8b186c364a1adcf83121e6898721d43", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 867054, "stop": 867878, "observations": { "mutation": [ 867143, 867149, 867359 ], "stop": [ 867143, 867149, 867359 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFV9", "query_cov": 1.0, "subject_cov": 0.5620915032679739, "identity": 1.0, "score": 318.0, "evalue": 1.26e-107, "gene_start": 1, "gene_end": 172, "reference_start": 2, "reference_end": 173, "reference_sequence": "MMVSKNVITILENSIKNNKLSHCYLLKAPSGLNIDDSILYMINKLCNSQLQSLDLETMLPNVQIFEDNGTENNLKKDKIVAGFEESSLSSFVEGQKKITVFKNVENASKAALNSLLKIIEEPSDNVVFILTTNNITKVLETIKSRSITININAPSNEELAEELKGENLKEEAIWFYSHIFADLTQVKKYTNNESFKLIKDLLEAIEKSIKNPHYLYIFLSRFTKKDSKDTFVFLAMALRFVFSWVWTADAFCRKNYAKLLVKLKNIKFDLFNCFIVIDDFLKIQNTNANFFLQAEKMLIKLVESYA" }, "paralog": false, "identity": 0.9854014598540146, "score": 520.0, "evalue": 9.71e-190, "description": "Internal stop codon at 867143, 867149, 867359. Nonsense mutation around 867143, 867149, 867359.", "psc": { "uniref90_id": "UniRef90_C4XFV9", "product": "DNA polymerase III subunit delta", "uniref50_id": "UniRef50_C4XFV9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFV9", "UniRef:UniRef50_C4XFV9" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFV9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFV9" ], "product": "DNA polymerase III subunit delta" } }, "id": "OAIKOHHJFB_1559", "locus": "GCA000209735_01554" }, { "type": "cds", "sequence": "contig_1", "start": 867893, "stop": 868249, "strand": "-", "frame": 1, "gene": "tmk", "product": "dTMP kinase", "db_xrefs": [ "EC:2.7.4.9", "GO:0004798", "GO:0005524", "GO:0006233", "KEGG:K00943", "SO:0001217", "UniRef:UniRef50_Q4A6Z8", "UniRef:UniRef90_C4XFW0" ], "nt": "TTGTGCGATCGCTATGTCGACAGCTTTTTTGCATATCAAGGTTATGCTCGTAATTTAGGTATTACTTTTGTTAAAAAACTTACTAATTTAATAATTGATAAAACTATGCCTGATATTACAATCTTTTTTGAAATCAAACCTGAAGAAAGTAAAATCAGAAGAGAACAAATAAGAATTGTTAGTGACAGATTAGACAGTGAAACCGAATTATTCCACCAAAAAGTTTACAAAGGTTATCATGAGCTAATTAAAAAAGAAAAAGATAGATTCATTGTTGTTGATGCTACAAAAAGTATTGAAGAAGTTTTAAAAGAATCATTTGAAAAATTAGATAAACATCCAAGATTTAAAAAATGA", "aa": "MCDRYVDSFFAYQGYARNLGITFVKKLTNLIIDKTMPDITIFFEIKPEESKIRREQIRIVSDRLDSETELFHQKVYKGYHELIKKEKDRFIVVDATKSIEEVLKESFEKLDKHPRFKK", "aa_hexdigest": "59c1993f650aa9d68c50d1277b0c8e06", "start_type": "TTG", "rbs_motif": null, "genes": [ "tmk" ], "truncated": "both", "pseudogene": { "start": 867875, "stop": 868537, "observations": { "mutation": [ 867895, 868279 ], "stop": [ 867895, 868279 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFW0", "query_cov": 1.0, "subject_cov": 0.5363636363636364, "identity": 0.992, "score": 228.0, "evalue": 3.12e-74, "gene_start": 1, "gene_end": 118, "reference_start": 97, "reference_end": 214, "reference_sequence": "MIMFITFEGPDGSGKTTIINRLIIELIEKYPKLALNYVLTREPGGKDIIEAEKIRSLILDKDSKLSSVSEALLYTTSRRIHLERVVWPALKENRLVLCDRYVDSFFAYQGYARNLGITFVKKLTNLIIDKTMPDITIFFEIKPEESKIRREQIRIVSDRLDSETELFHQKVYKGYHELIKKEKDRFIVVDATKSIEEVLKESFEKLDKHPRFKKWIKEFK" }, "paralog": false, "identity": 0.9863636363636363, "score": 422.0, "evalue": 4.99e-153, "description": "Internal stop codon at 867895, 868279. Nonsense mutation around 867895, 868279.", "psc": { "uniref90_id": "UniRef90_C4XFW0", "gene": "tmk", "product": "dTMP kinase", "ec_ids": [ "2.7.4.9" ], "uniref50_id": "UniRef50_Q4A6Z8", "kegg_orthology_id": "K00943", "go_ids": [ "GO:0004798", "GO:0005524", "GO:0006233" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFW0", "EC:2.7.4.9", "UniRef:UniRef50_Q4A6Z8", "KEGG:K00943", "GO:0004798", "GO:0005524", "GO:0006233" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A6Z8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6Z8" ], "product": "Thymidylate kinase" } }, "id": "OAIKOHHJFB_1560", "locus": "GCA000209735_01555" }, { "type": "cds", "sequence": "contig_1", "start": 868277, "stop": 868531, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02223.23" ], "nt": "ATGTTTATTACTTTTGAAGGACCAGATGGTAGTGGTAAAACTACAATAATTAACCGCCTTATTATTGAATTAATTGAAAAATATCCAAAGCTTGCATTAAATTATGTTTTGACTCGTGAACCGGGTGGCAAAGACATAATTGAAGCAGAAAAAATTAGATCATTAATTTTGGATAAAGATTCAAAGCTTTCTTCAGTTTCTGAAGCTCTTTTATATACAACTAGTCGTCGAATTCATCTTGAAAGAGTAGTATGA", "aa": "MFITFEGPDGSGKTTIINRLIIELIEKYPKLALNYVLTREPGGKDIIEAEKIRSLILDKDSKLSSVSEALLYTTSRRIHLERVV", "aa_hexdigest": "00831f06906d099994faeb4c3b971296", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02223.23", "name": "Thymidylate_kin", "length": 79, "aa_cov": 0.9404761904761905, "hmm_cov": 0.9113924050632911, "evalue": 2.1598140458842297e-14, "score": 49.67196273803711, "start": 5, "stop": 83 } ], "seq_stats": { "molecular_weight": 9517.996399999996, "isoelectric_point": 8.0078275680542 }, "id": "OAIKOHHJFB_1561", "locus": "GCA000209735_01556" }, { "type": "cds", "sequence": "contig_1", "start": 868551, "stop": 869141, "strand": "-", "frame": 3, "gene": "recR", "product": "Recombination protein RecR", "db_xrefs": [ "KEGG:K06187", "RefSeq:WP_013354986.1", "SO:0001217", "UniParc:UPI0001A8C12E", "UniRef:UniRef100_C4XFW1", "UniRef:UniRef50_N9VD56", "UniRef:UniRef90_C4XFW1" ], "nt": "ATGAAAATTGAAACAATTGAAAAACTTAATCAAGTTTTAACAACAATTCCAGGAATTAGTAAAAAGCAAGCAGACAAAATGTCAAACTTTTTCTTAAAACAAGATCAAGAATATATTGACAATTTAGTTAAGTCAATTAATGATCTTAAAACCAACATTTCATATTGTTCTATTTGTAACTTTTTAAAAGAAGGTGGCAAATGTTTAGTATGTGATGATAAGAGCAGATACAACTTTTTAATGATAGTTGAAAATGCTGCAACTTTAAGAAAAATTGATGACATGAATTTTTACAATGGCTACTACTATGTTTTGCCTTATTTGTTTAATTTAAAGAATAATAAGGAAATTAAAAACTATGATTACAAAGAATTAATAAACTATATTCAACATAAAAAATTTGATGAAGTAATCATAGTTTTGAGCCCTTCTTTAGAAGGCGAAATGACAACAGGTCATTTATTAAAATTGATTAATAGCCAATTTAAAATTAAAGTAACAAGAGCAGCTATTGGCATGCCTATGGGTTCAAATTTAGAATATCTTGATACTTTCACAATCAAACAATCTATTGAAAATAGAAACAAATAA", "aa": "MKIETIEKLNQVLTTIPGISKKQADKMSNFFLKQDQEYIDNLVKSINDLKTNISYCSICNFLKEGGKCLVCDDKSRYNFLMIVENAATLRKIDDMNFYNGYYYVLPYLFNLKNNKEIKNYDYKELINYIQHKKFDEVIIVLSPSLEGEMTTGHLLKLINSQFKIKVTRAAIGMPMGSNLEYLDTFTIKQSIENRNK", "aa_hexdigest": "723c7d4573e29f587df86bd8be7458b4", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C12E", "ncbi_nrp_id": "WP_013354986.1", "uniref100_id": "UniRef100_C4XFW1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C12E", "RefSeq:WP_013354986.1", "UniRef:UniRef100_C4XFW1" ] }, "ips": { "uniref100_id": "UniRef100_C4XFW1", "uniref90_id": "UniRef90_C4XFW1" }, "psc": { "uniref90_id": "UniRef90_C4XFW1", "product": "Recombination protein RecR", "uniref50_id": "UniRef50_N9VD56", "kegg_orthology_id": "K06187" }, "pscc": { "uniref50_id": "UniRef50_N9VD56", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9VD56" ], "product": "Recombination protein RecR" }, "genes": [ "recR" ], "id": "OAIKOHHJFB_1562", "locus": "GCA000209735_01557" }, { "type": "cds", "sequence": "contig_1", "start": 869144, "stop": 869440, "strand": "-", "frame": 1, "gene": null, "product": "Nucleoid-associated protein MBIO_0769", "db_xrefs": [ "KEGG:K09747", "RefSeq:WP_013354985.1", "SO:0001217", "UniParc:UPI0001E33020", "UniRef:UniRef100_C4XFW2", "UniRef:UniRef50_C4XFW2", "UniRef:UniRef90_C4XFW2" ], "nt": "ATGGATCAAGCAATGTTAAGAAAGATGCAAAAATTGCAACAAGAAGTTCAAGAAAAAACTGAACAATTCATGGAAGAAACATTTAAATTAGAAAAACATGGAATTGAAGTTACAGCTAAAGGAAGCAAAAAAATAGTTTCAGTAAAAATCAAAGATTCTATTCTTTTAGACCCAGATGATGCTGAAACTTTAGAAGACTTGTTATCACTTGTGATTAATGAGTTATTTGACACAATTGATGAAGAACAAGAAAAATTAATGCCAGCTATGCCTGGTTTAGGTGGACTTGGCTTCTAA", "aa": "MDQAMLRKMQKLQQEVQEKTEQFMEETFKLEKHGIEVTAKGSKKIVSVKIKDSILLDPDDAETLEDLLSLVINELFDTIDEEQEKLMPAMPGLGGLGF", "aa_hexdigest": "ab897048bca2ffa3e26156e29058872f", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E33020", "ncbi_nrp_id": "WP_013354985.1", "uniref100_id": "UniRef100_C4XFW2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33020", "RefSeq:WP_013354985.1", "UniRef:UniRef100_C4XFW2" ] }, "ips": { "uniref100_id": "UniRef100_C4XFW2", "uniref90_id": "UniRef90_C4XFW2" }, "psc": { "uniref90_id": "UniRef90_C4XFW2", "product": "Nucleoid-associated protein MBIO_0769", "uniref50_id": "UniRef50_C4XFW2", "kegg_orthology_id": "K09747" }, "pscc": { "uniref50_id": "UniRef50_C4XFW2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFW2" ], "product": "Nucleoid-associated protein MBIO_0769" }, "genes": [], "id": "OAIKOHHJFB_1563", "locus": "GCA000209735_01558" }, { "type": "cds", "sequence": "contig_1", "start": 869488, "stop": 871302, "strand": "-", "frame": 2, "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "db_xrefs": [ "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0009360", "KEGG:K02343", "RefSeq:WP_013527161.1", "SO:0001217", "UniParc:UPI0001F5992C", "UniRef:UniRef100_C4XFW3", "UniRef:UniRef50_A0A6M4J8T6", "UniRef:UniRef90_C4XFW3" ], "nt": "ATGGCATATAAAGCACTTTATCGAAAATATCGTCCGAAGTCTTTTGATGATGTTAAGGGTCAAGATCATATTGTGCAAACATTAAAAAATATCATTTTAAATCACAAGATAAGTCATGCATACTTATTTAGTGGACCAAGAGGTGTTGGTAAAACTTCAGTTGCTAAAATTTTTGCTAATGTAGTTAACTGTGGTCATGGTGAAGATTTAACTAAACTTTGTGAAAGTTGTATTGAAAAAGCGGATCAAAATTTTGATGTTATCGAAATGGATGCTGCTTCAAATAATGGTGTTAATGAAATTAGAGATTTAAGAGATAAAATCCAACATTCACCAGCAAGTGGAAACTACAAAGTTTATATAATCGATGAAGTTCACATGCTTTCAAAAGGAGCTTTTAATGCACTTTTAAAAACATTAGAAGAACCACCAGCACATGCAATATTTATTTTAGCAACAACTGATCCTCAAAAAATACCTCTAACAATTCTTTCAAGAGTCCAAAGATATAATTTTAGGAAAATATCAAACCAAGTTCTAATTAACCAAATTAAAGAGGTCTTAGATAAAGAAAAAATTAAATATGAAGATTCAACCATAGCTTATATTGCAAGATTAGCAACTGGTGGTATGCGTGATGCACTTTCAATAGTCGACCAAGCTGCAGCTTATGGTAACGACAAAATATCGCTCAAAGATGTTATGTATGCTTTTGGAATTACTTCAAATGAAAGCTTAATTCAAATTGTAAACTACCTTTATCAAGGCAAAATCAAGGAAGTCTTAATTTTATTTAATAACCTTAAAAATGCGGGAATTGATTCAAATCAATTTGTTCAAAGCTTAATAGAATTGTTCAAGGATTACTTGATTTATGCAAAATCAAATGATGCTAATTTATTAGAACTTTTATCTCTTGAAGAATTAAATACTTTGAAAATTGATGTTAATTTTGCATTCAATTCATCTGAAAAACTTTATAAATTATTCAAAGAATTAATTTTTTCAGAAAACCCATTCCAATTAATCGAACTTTATTTAATTAAACTTAATCAAACTAATGAATTACAAAATAGTAAGGAGCAAACTATGTCAATTGATGTAAACAAAAAGAAACCTAAAAAAGATGACACAATCAAAACAGTTTTAAATCAAACTCAAGAATTAGTACTTGAAGCTAATCGTGACGCTGATGACAATTTTAGCCTAGAACAAGATCTTCTCAGTAGCAATTTTGAAATAGAAGAAGATTATGATGATGGTTTAGTAAGTACAGCAGAAATTCAACTAGATGATGAAATTCAAAATATGCCAAAACCAGTAGTAATCCCTGCTTTTGACTCAAAATACAAAAACTTGAAAACATTCAAATCAAACTTAAGTAAAAAAGATTTAATCAACTTACTTAAACAATCAACATTAACATTTTTAAATAGATACAAATCAAATTTAGATATTGTGATTAACACAGTTCAAAATCCAATTTACAGTGATTTAATTACAGTACTTAAAAAATGTAAAATTGCCGCAGCAGGCCCTAATTACATGATTTTTATCTCAAAAAATGTACCAGAATTAGTTTATTTACAAGAAAATGGTTACAACTCAAATATTCAAAAATTTATTCAAGATTACTTTGAAGATTACAAACACTTATACTGCATTATGAGTGATGATTACAAAGAAACAGCAAAAGATTTTGCAGCTAAAATGAAAGATGCAAAATTTGACACAAGCTTTACAGTACCAGGACCAGTTGTTTTACAAAATGTAAAGAGTCCAACAGGCAAATTATTTGATAAATTCAAAAAATAA", "aa": "MAYKALYRKYRPKSFDDVKGQDHIVQTLKNIILNHKISHAYLFSGPRGVGKTSVAKIFANVVNCGHGEDLTKLCESCIEKADQNFDVIEMDAASNNGVNEIRDLRDKIQHSPASGNYKVYIIDEVHMLSKGAFNALLKTLEEPPAHAIFILATTDPQKIPLTILSRVQRYNFRKISNQVLINQIKEVLDKEKIKYEDSTIAYIARLATGGMRDALSIVDQAAAYGNDKISLKDVMYAFGITSNESLIQIVNYLYQGKIKEVLILFNNLKNAGIDSNQFVQSLIELFKDYLIYAKSNDANLLELLSLEELNTLKIDVNFAFNSSEKLYKLFKELIFSENPFQLIELYLIKLNQTNELQNSKEQTMSIDVNKKKPKKDDTIKTVLNQTQELVLEANRDADDNFSLEQDLLSSNFEIEEDYDDGLVSTAEIQLDDEIQNMPKPVVIPAFDSKYKNLKTFKSNLSKKDLINLLKQSTLTFLNRYKSNLDIVINTVQNPIYSDLITVLKKCKIAAAGPNYMIFISKNVPELVYLQENGYNSNIQKFIQDYFEDYKHLYCIMSDDYKETAKDFAAKMKDAKFDTSFTVPGPVVLQNVKSPTGKLFDKFKK", "aa_hexdigest": "7a64fe6ac682e2a25e81fcfbfc18e131", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001F5992C", "ncbi_nrp_id": "WP_013527161.1", "uniref100_id": "UniRef100_C4XFW3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5992C", "RefSeq:WP_013527161.1", "UniRef:UniRef100_C4XFW3" ] }, "ips": { "uniref100_id": "UniRef100_C4XFW3", "uniref90_id": "UniRef90_C4XFW3" }, "psc": { "uniref90_id": "UniRef90_C4XFW3", "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_A0A6M4J8T6", "kegg_orthology_id": "K02343", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6M4J8T6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6M4J8T6" ], "product": "DNA polymerase III subunit gamma/tau" }, "genes": [ "dnaX" ], "id": "OAIKOHHJFB_1564", "locus": "GCA000209735_01559" }, { "type": "cds", "sequence": "contig_1", "start": 871482, "stop": 872006, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002079168B" ], "nt": "ATGTCTGAAATAATAAAAGTTGAAAATTTAAAAAAATCATATGGCAAAGTTGAAGCTGTCAAAGGTATTTCTTTCATTGTAAAAGAAGGAGAGCTTTTTGCATTTTTAGGGCCTAATGGTGCAGGCAAAAGTACCACAATTGATACCTTGTGTACATTGAATGATTATAATGAAGGCCAAATAACAATTTGTGGTTTTGATTTAAAGAAACAAAGTGATGAAATCAAAAAACAAATTGGTGTGGTTTTTCAAGATAGTGTATTAGATCGAAAATTAAGTGTTAGAGATAATTTAACAATTAGAGCAGCATATTATTATCGTAAAAAAGCAGATATAAAAGCAGCTTTTGAAAGGGTTGTAAAAGATACTCAAATTGAAGATATTTTGAAAAGAAAATATGAAAAACTTTCAGGTGGTCAAAGACGTCGTGTGGATATAGCTAGAGCCTTATTAAATTATCCAAAAATTTTATTTTTAGATGAACCAACAACAGGATTAGATCCTCAAACTAGAAAGCTTATTTGA", "aa": "MSEIIKVENLKKSYGKVEAVKGISFIVKEGELFAFLGPNGAGKSTTIDTLCTLNDYNEGQITICGFDLKKQSDEIKKQIGVVFQDSVLDRKLSVRDNLTIRAAYYYRKKADIKAAFERVVKDTQIEDILKRKYEKLSGGQRRRVDIARALLNYPKILFLDEPTTGLDPQTRKLI", "aa_hexdigest": "bb0ae2805b7984ffea7308ee2bf28558", "start_type": "ATG", "rbs_motif": "AGGAGG", "psc": { "uniref90_id": "UniRef90_UPI002079168B", "query_cov": 1.0, "subject_cov": 0.8055555555555556, "identity": 0.632, "score": 223.0, "evalue": 2.44e-71, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI002079168B" }, "pscc": { "uniref50_id": "UniRef50_UPI002079168B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002079168B" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "OAIKOHHJFB_1565", "locus": "GCA000209735_01560" }, { "type": "cds", "sequence": "contig_1", "start": 872067, "stop": 872402, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGAAGCTGAAAAAGCTGACTATGTTGTGATTATGGATAATGGAAAAATTGTGGCTAAGAATACTCCTGCAAAACTAAAAGAAGAATATACAAAAGATAGGTTAAAAGTATATCTTGAAAATGATGTAAAGTACTTTAAAACAGTTGAATTGTTAAAAAACAAAGGTTTAAAATTCAAAGAATTTAAAGATTATATTGATATATATTTAGACTCTTCGTTTAAAGCTATAGATTTGGCAAATCAACTTAAAGATTTGATATCTTCTTTCGAAGTTTTAAAGGGTGATATGGATGATGTATTTATCAATATAACTGGAAAGGAGATTAGATAA", "aa": "MEEAEKADYVVIMDNGKIVAKNTPAKLKEEYTKDRLKVYLENDVKYFKTVELLKNKGLKFKEFKDYIDIYLDSSFKAIDLANQLKDLISSFEVLKGDMDDVFINITGKEIR", "aa_hexdigest": "9fb27126814fa2593e4fe5f90b52dca4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12938.804299999985, "isoelectric_point": 5.269167900085448 }, "id": "OAIKOHHJFB_1566", "locus": "GCA000209735_01561" }, { "type": "cds", "sequence": "contig_1", "start": 872404, "stop": 872547, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGCAATAATTAAAAGAAATATTAAAAAATATTTCATGGACAAAACTAATGTTTTCTTTTCTTTATTAAGTGTTATTTTAGTCTTCTTTATAGTTGCAGTTTTTTTAGGTAAATCAATTAGTGATGGACTTAAAGGATGA", "aa": "MGAIIKRNIKKYFMDKTNVFFSLLSVILVFFIVAVFLGKSISDGLKG", "aa_hexdigest": "047a109a396dc4c8a91c232e14e0c49c", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5270.385499999999, "isoelectric_point": 10.121810340881346 }, "id": "OAIKOHHJFB_1567", "locus": "GCA000209735_01562" }, { "type": "cds", "sequence": "contig_1", "start": 872596, "stop": 873027, "strand": "+", "frame": 1, "gene": null, "product": "ABC-2 type transporter transmembrane domain-containing protein", "db_xrefs": [ "KEGG:K11051", "SO:0001217", "UniRef:UniRef50_C4XFW5", "UniRef:UniRef90_C4XFW5" ], "nt": "ATGATTTCAACTACAACGACAATTGGTTGTATTACAGTTAGTTTAGGTGCTTCAAGTTTCATGGTTGAAGATATAGAATCAAAAGCCTATAAAGACATTTATGCTTCGCCAGTTAATAAATCTTCAATAATTATTGGAAACGCTATTTCTATTTATGCTGTTACTGTAATTATGTCAACTGTATCATTTATTATTAGTTTTGTGTATGCATTATGCTTTGGAGCAAAATTCTTATCATTTTTACAAATTGTTGAAGTAATTGGATTGATTTTAGTTTCAAGTTTAATAGGAAGTAGTTTTAGCTTTTTAATTGTTTACTTCATTAAAAAACATAATGTTTTTGCAGCTATCAATGCTTTAATTGGATCTATAGCAGGTTTTATAATTGGTGGTTATTCAACAATGGAATCACTGCCTGATGCGCTTCAATGA", "aa": "MISTTTTIGCITVSLGASSFMVEDIESKAYKDIYASPVNKSSIIIGNAISIYAVTVIMSTVSFIISFVYALCFGAKFLSFLQIVEVIGLILVSSLIGSSFSFLIVYFIKKHNVFAAINALIGSIAGFIIGGYSTMESLPDALQ", "aa_hexdigest": "dd84a2f864aefc33be0e99c0a1821b88", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 872383, "stop": 873276, "observations": { "mutation": [ 872545, 872593, 873025, 873184 ], "stop": [ 872545, 872593, 873025, 873184 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFW5", "query_cov": 1.0, "subject_cov": 0.48148148148148145, "identity": 1.0, "score": 256.0, "evalue": 5.89e-84, "gene_start": 1, "gene_end": 143, "reference_start": 72, "reference_end": 214, "reference_sequence": "MERRLDNMGAIIKRNIKKYFMDKTNVFFSLLSVILVFFIVAVFLGKSISDGLKGWVIEDKSKSAREIMNLWMISTTTTIGCITVSLGASSFMVEDIESKAYKDIYASPVNKSSIIIGNAISIYAVTVIMSTVSFIISFVYALCFGAKFLSFLQIVEVIGLILVSSLIGSSFSFLIVYFIKKHNVFAAINALIGSIAGFIIGGYSTMESLPDALQWIIKCFPASHANVLFRQTLVSPAIDRLISDPTHIEQFKKIMGCFYKFNDYQMQWYVNFIIVAGTSLLLYSIAFGVQFTKKIKN" }, "paralog": false, "identity": 0.9831649831649831, "score": 553.0, "evalue": 1.8e-203, "description": "Internal stop codon at 872545, 872593, 873025, 873184. Nonsense mutation around 872545, 872593, 873025, 873184.", "psc": { "uniref90_id": "UniRef90_C4XFW5", "product": "ABC-2 type transporter transmembrane domain-containing protein", "uniref50_id": "UniRef50_C4XFW5", "kegg_orthology_id": "K11051", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFW5", "UniRef:UniRef50_C4XFW5", "KEGG:K11051" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFW5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFW5" ], "product": "ABC-2 type transporter transmembrane domain-containing protein" } }, "id": "OAIKOHHJFB_1568", "locus": "GCA000209735_01563" }, { "type": "cds", "sequence": "contig_1", "start": 873322, "stop": 874029, "strand": "-", "frame": 2, "gene": null, "product": "Uridine phosphorylase", "db_xrefs": [ "EC:2.4.2.1", "KEGG:K03784", "RefSeq:WP_013354980.1", "SO:0001217", "UniParc:UPI0001E3301B", "UniRef:UniRef100_C4XFW6", "UniRef:UniRef50_A0A449B495", "UniRef:UniRef90_C4XFW6" ], "nt": "ATGTCAACACACATCAGCGCAAAAAAAGGTGAAATAGCAAGAATTTGTTTTCTTTCAGGAGACCCTTTAAGATCAAAATTTATGGCTCATAAATATTTAAAAAATGTAAAAGAAGTAAGCTCAGTTAGAAATGAAAATTACTACACAGGAGAATATAGAGGTATCAAAGTTACTTTTGGAGGACATGGAATGGGTATGTTCTCAATTGGAGCTTATGCTCATGAACTTTATAATGAATATGGAGTAGATGTTATTATTCGTTTAGGATCAACTGGTTCATATTATGACGAACACAATGTTATGGATACAGTAATTATTAATAGAGCTTTTAGCGACAACATTTCAATAGCTCAATTAATAAATAAAGAAAATACATATGAATACTTTCCAGACAAAGGTGTAACAGATGAATTAATTAAGGCAGCTAAAAAATTAAACAAAAAAGCACCAGTAGTTTCAGTACACTCAACAGATGTTTTCTATGCAACTAGACCTTTAGAGGAAACTAAGAAAGTATCAAAATGTCAAGTAGTTGAAGCAGAAAGTTATGCGCTTTTTGCAGAAGCTAAAAGAAGCAATAAAAAAGCCGCAACCATTTTACAAGTTAGTGACTCTTTAGCTAAAATGGAATTTAGTGATTATAAAACTAGAGAAACAGGATTTACAGAAATGTTCGAAATAGCTTTGGAAGCATTAAAAAATCTATAA", "aa": "MSTHISAKKGEIARICFLSGDPLRSKFMAHKYLKNVKEVSSVRNENYYTGEYRGIKVTFGGHGMGMFSIGAYAHELYNEYGVDVIIRLGSTGSYYDEHNVMDTVIINRAFSDNISIAQLINKENTYEYFPDKGVTDELIKAAKKLNKKAPVVSVHSTDVFYATRPLEETKKVSKCQVVEAESYALFAEAKRSNKKAATILQVSDSLAKMEFSDYKTRETGFTEMFEIALEALKNL", "aa_hexdigest": "3f221302d8220e1f34074f361ffa8ba9", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E3301B", "ncbi_nrp_id": "WP_013354980.1", "uniref100_id": "UniRef100_C4XFW6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E3301B", "RefSeq:WP_013354980.1", "UniRef:UniRef100_C4XFW6" ] }, "ips": { "uniref100_id": "UniRef100_C4XFW6", "uniref90_id": "UniRef90_C4XFW6" }, "psc": { "uniref90_id": "UniRef90_C4XFW6", "product": "Uridine phosphorylase", "ec_ids": [ "2.4.2.1" ], "uniref50_id": "UniRef50_A0A449B495", "kegg_orthology_id": "K03784" }, "pscc": { "uniref50_id": "UniRef50_A0A449B495", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B495" ], "product": "Uridine phosphorylase" }, "genes": [], "id": "OAIKOHHJFB_1569", "locus": "GCA000209735_01564" }, { "type": "cds", "sequence": "contig_1", "start": 874306, "stop": 874578, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAAAAAAAGAAAATTATTCTTAATCCTATCTTCATTAAGTACATTACCTTTTGCTTTTGTAGCAGGTAGTTGTGTTGGTAAAGATAATGGTGGAACAACCGGTAAACCAACTAGTGAAGAAACAAAAGCTGATTTAAATAAACTTGCAAAAAATATTGTTTTTGATGTTAAAAATAAACAAGATAAATTTGCAAAGGATATTAAAAACATATCAAATTTAGTTGTATCAAATTTAGATTTAAATAAATATGAATTAATACATTGA", "aa": "MKNKKRKLFLILSSLSTLPFAFVAGSCVGKDNGGTTGKPTSEETKADLNKLAKNIVFDVKNKQDKFAKDIKNISNLVVSNLDLNKYELIH", "aa_hexdigest": "01c14e5c21a633209d8d8915430d3261", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9955.469499999994, "isoelectric_point": 9.678977012634277 }, "id": "OAIKOHHJFB_1570", "locus": "GCA000209735_01565" }, { "type": "cds", "sequence": "contig_1", "start": 874891, "stop": 875160, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATACTGCAACAGGCGATTTATATATATCGTTTAAATTAAGAGAAAAAGCAAATAATATTTCTGTTGACGGTTATTGTAAAGTTCAAGGTTTCAAACCATTTGATAGTGTTAAAAATCCTGATTTAATAGATGTAGCTAATGCAGCAAAAGCTGTAAATGTTGCGTATGAAGATAAAGGTAGCTTTTCTATTTATGAATATAATGCGAATTTAGTTAAATTTGAAAATTATGATTCATCTATTTATGATTTAAGCCTAGAATCTTGA", "aa": "MDTATGDLYISFKLREKANNISVDGYCKVQGFKPFDSVKNPDLIDVANAAKAVNVAYEDKGSFSIYEYNANLVKFENYDSSIYDLSLES", "aa_hexdigest": "e8eeb3464227932a61b6f2ec77407e02", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9949.9192, "isoelectric_point": 4.4735925674438475 }, "id": "OAIKOHHJFB_1571", "locus": "GCA000209735_01566" }, { "type": "cds", "sequence": "contig_1", "start": 875329, "stop": 875481, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCCACTACACAAAAAAGACCAACATTTAGAATATCTAAAGAATTAGATGCAATTTTTGACAAAGCAAAAGAGTTTTATCTAAAACCAGAAAACTTCGATATTTACACAGGAGAATTTAAAACACAAGTTTATGGAAAATATAGTTCTTGA", "aa": "MPTTQKRPTFRISKELDAIFDKAKEFYLKPENFDIYTGEFKTQVYGKYSS", "aa_hexdigest": "0a849c2c74d04a35bfc30c682be98b1b", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5958.704200000001, "isoelectric_point": 9.003799629211429 }, "id": "OAIKOHHJFB_1572", "locus": "GCA000209735_01567" }, { "type": "cds", "sequence": "contig_1", "start": 875572, "stop": 875892, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAACGAGCTTTTATATATAATAAAACTAAATTTGTTGTAACTGATTACACAATTAATGGTGATAAAGTAGAATTAAAATTTAAACTTTGTCGTAATTTAGATGATAAAAGTAAAAAGTCATTTTATGAAATATCAACAAAAGAATTCACAAAAGAAATTACTTTAGCTACAGAAAAAACAACAGAAAAAGCATATTTGGACAAACTAATTGATAATTATGACTTTTCACTTCCAGAAGGCTTACAACAATATCATGCCCATAAAGTTCTTTCTCAATTAAAACAAAAACAAATAGGATTTTTTGAACAAAAAAAATGA", "aa": "MERAFIYNKTKFVVTDYTINGDKVELKFKLCRNLDDKSKKSFYEISTKEFTKEITLATEKTTEKAYLDKLIDNYDFSLPEGLQQYHAHKVLSQLKQKQIGFFEQKK", "aa_hexdigest": "17b454cd7f35128e16eb2c479cc310fe", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12600.306499999988, "isoelectric_point": 9.060338401794432 }, "id": "OAIKOHHJFB_1573", "locus": "GCA000209735_01568" }, { "type": "cds", "sequence": "contig_1", "start": 876070, "stop": 876210, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAATTTAATAACTTTAAAGATTTAAATCCACTTATTAAATTAGTAGACAGATTGAATAAGTCAATGGTAGCTTATATTAATCCAAATGAAAGTCTTGATACATTTAATGAATCATTAGGACGTCAATTAAAATCTTGA", "aa": "MQFNNFKDLNPLIKLVDRLNKSMVAYINPNESLDTFNESLGRQLKS", "aa_hexdigest": "7abc8d783cdf9c2038af64404423c54f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5357.082399999999, "isoelectric_point": 8.139600944519042 }, "id": "OAIKOHHJFB_1574", "locus": "GCA000209735_01569" }, { "type": "cds", "sequence": "contig_1", "start": 876211, "stop": 876318, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGTAGTAATCTTTTTTATACAGTATCAAATAAATTTAAAGCAATAGATTTTACAGATCAAAGTGCTTTAAATGAAATTCAAAGTTATTATGATAATGAATGA", "aa": "MNGSNLFYTVSNKFKAIDFTDQSALNEIQSYYDNE", "aa_hexdigest": "03a5e017bc5a35d1e2d864163aa158e4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4068.344, "isoelectric_point": 4.295857048034668 }, "id": "OAIKOHHJFB_1575", "locus": "GCA000209735_01570" }, { "type": "cds", "sequence": "contig_1", "start": 876484, "stop": 876996, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAGCAATTAAATTAACACATAATTCAATTAATTCGCCAAAAACCTTTGATCCAAAAATTAATAATAGTTTTCCATTCACAGATTCGAAACTTGAAGAATATGTTAAAACAGACGATTTTAAACACAATTTAGAAGCAAATATTAAAGAAAACTTTTATTTACTTGATTTCATTAAAAAAGAAACTTCTGATAATAAAAAAGATATAAGCAAATTTGTACTTTCTGATTTAGAACTACAAAAAGAATATGATTCAATATTGTTAGATGAAGAATCAAAAAAGATCATTGTTACAAATATTGAAAATTATTTAAATAATAAATTTGATTCTAAATTTAATAAATTTTTAAATTCATATTTCAAACAAGCAGAAATAGCTAGAAAGAACAAAGCGATTAAAGATGATCAAAAATTGTTTAGATATACAATTAAAGAATTCTCAAATATTGATGCCTATCAATCTAAAATTAAAATTCAAGTTGAAATATTCAAAATAGAAGCTAAGGCTTGA", "aa": "MEAIKLTHNSINSPKTFDPKINNSFPFTDSKLEEYVKTDDFKHNLEANIKENFYLLDFIKKETSDNKKDISKFVLSDLELQKEYDSILLDEESKKIIVTNIENYLNNKFDSKFNKFLNSYFKQAEIARKNKAIKDDQKLFRYTIKEFSNIDAYQSKIKIQVEIFKIEAKA", "aa_hexdigest": "1914aaf09749d3d7c07f41e9e9a47405", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20145.6631, "isoelectric_point": 7.748711967468262 }, "id": "OAIKOHHJFB_1576", "locus": "GCA000209735_01571" }, { "type": "cds", "sequence": "contig_1", "start": 877144, "stop": 877518, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGCATATGGTGTTGAACAAGCTATGTCATCTAAAGCAGACAAACTAGAATTCCTTGAAAAATTAATGTCTGTTGGTGGTGCTAAAATAAAAGAACATGATTCAGCTATTTCCTTTATTAATGCTTATTTATTCAAGATGTTAATTTTCGAAAATGTTAAAGTTTATGAAAGACTTAATCGATTTGACAAAGTAGAATTTACATATGATCAAGTAAATAACAAAGTTGTTGTTAAATTGGTATTTGGATATGAAACACATGTATTTAATATTGATAGCAATAAATTTGACAAGAAAGTTTTAAATGTAATTAAAGTTTTTGCAAACAATGAGTTAAATAAATTTATTGATGAAATTATAAAAAATAATTGA", "aa": "MSAYGVEQAMSSKADKLEFLEKLMSVGGAKIKEHDSAISFINAYLFKMLIFENVKVYERLNRFDKVEFTYDQVNNKVVVKLVFGYETHVFNIDSNKFDKKVLNVIKVFANNELNKFIDEIIKNN", "aa_hexdigest": "32e5becb6d9cbb74d1538c160e75b8c1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14413.498099999983, "isoelectric_point": 7.861742210388185 }, "id": "OAIKOHHJFB_1577", "locus": "GCA000209735_01572" }, { "type": "cds", "sequence": "contig_1", "start": 877687, "stop": 877797, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAGAAATGTATGGATATAGTTTACCTTATGTAACCGATGGAATTAATTTAGATTTTGATATAGATAAGTTTCTAAGCCCAGTAGCTTCACAACTTAAAGGTATTTGA", "aa": "MREMYGYSLPYVTDGINLDFDIDKFLSPVASQLKGI", "aa_hexdigest": "90fbb94d0c7ef1859795a0fd97031ec3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4097.6634, "isoelectric_point": 4.359459877014161 }, "id": "OAIKOHHJFB_1578", "locus": "GCA000209735_01573" }, { "type": "tRNA", "sequence": "contig_1", "start": 878174, "stop": 878248, "strand": "-", "gene": null, "product": "tRNA-Xxx", "score": 73.7, "nt": "AGGAGTATAGTTCAATGGTAGAACAACGGGCTTCAACCCCGTGTGTTGTGGGTTCGAGTCCTGCTACTCCTGCCA", "db_xrefs": [], "id": "OAIKOHHJFB_18", "locus": "GCA000209735_01574" }, { "type": "cds", "sequence": "contig_1", "start": 878281, "stop": 878514, "strand": "-", "frame": 2, "gene": null, "product": "Carrier domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354978.1", "SO:0001217", "UniParc:UPI0001E33019", "UniRef:UniRef100_C4XFW8", "UniRef:UniRef50_A0A3P8MEJ4", "UniRef:UniRef90_C4XFW8" ], "nt": "ATGGAAAAAAATAAAATTAAAGAAATTGTTATTGCAAGATTAGACAAACTTTCAAAGAAAAAGGTAACTTTTACTTCAACACTCGAAGAATTAAAAATTGATTCATTATCACTTGCTGAATTAATTTACGAAAGTGAAATAGAATTCAAAATTAGAATTGAAGATGAAGTGCTTTTAAAAATTAAAAATGTTAATGATATTATTGATGCAATAGAAAATGCTAAGAAAATTTAA", "aa": "MEKNKIKEIVIARLDKLSKKKVTFTSTLEELKIDSLSLAELIYESEIEFKIRIEDEVLLKIKNVNDIIDAIENAKKI", "aa_hexdigest": "612627cfa794abc55c1790cbafd3c523", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E33019", "ncbi_nrp_id": "WP_013354978.1", "uniref100_id": "UniRef100_C4XFW8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E33019", "RefSeq:WP_013354978.1", "UniRef:UniRef100_C4XFW8" ] }, "ips": { "uniref100_id": "UniRef100_C4XFW8", "uniref90_id": "UniRef90_C4XFW8" }, "psc": { "uniref90_id": "UniRef90_C4XFW8", "product": "Carrier domain-containing protein", "uniref50_id": "UniRef50_A0A3P8MEJ4" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MEJ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MEJ4" ], "product": "Acyl carrier protein" }, "genes": [], "id": "OAIKOHHJFB_1579", "locus": "GCA000209735_01575" }, { "type": "cds", "sequence": "contig_1", "start": 878763, "stop": 879251, "strand": "-", "frame": 3, "gene": "prmC", "product": "peptide chain release factor N(5)-glutamine methyltransferase", "db_xrefs": [ "EC:2.1.1.297", "GO:0006412", "GO:0008757", "GO:0018364", "SO:0001217", "UniRef:UniRef50_A0A3P8KWM4", "UniRef:UniRef90_C4XFW9" ], "nt": "ATGAATAACGAAGAAACATTAATTTTAGAAAAAAAGCGGTATGGTTTGGAACCTAAAATTACTGAAAAAGAATATGAATTATTAAGGCAAAATTATCCAATTCAATTAATAATGGGCTATGTTGAATACTTAAATACTAGAATTAATTTAAGAGAACAAGTTTTAATTCCAAGATATGAAACTGAAGAAATGGTTGATATTTATCTAAAAGAATTTGCAGCTGAAAATCAAGAAGTTTTAGATCTTTGTTGTGGTTCAGGTTTTATAGGTTTAGCAATTAAGAAAAACTTACGTTCAGCTAAAGTAACTTTAGTAGATATTAGTGATGAAGCTATTAAACAAACCATTGAAAATTCTATAGTAAATTTTGGTTTTAATCATGACTTAAAAATTTACCAAAGTGACTTATTTACTAATGTTAAGGGAAGATTTGATGTTATCATTTCAAATCCACCATATTTAAATTGCAATGACAAAAGTTTAGACTGA", "aa": "MNNEETLILEKKRYGLEPKITEKEYELLRQNYPIQLIMGYVEYLNTRINLREQVLIPRYETEEMVDIYLKEFAAENQEVLDLCCGSGFIGLAIKKNLRSAKVTLVDISDEAIKQTIENSIVNFGFNHDLKIYQSDLFTNVKGRFDVIISNPPYLNCNDKSLD", "aa_hexdigest": "4d2d5d1b25be6a22fda828c18050ecd3", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "genes": [ "prmC" ], "truncated": "5-prime", "pseudogene": { "start": 878538, "stop": 879263, "observations": { "mutation": [ 878615, 878654, 878711, 878765 ], "stop": [ 878615, 878654, 878711, 878765 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFW9", "query_cov": 1.0, "subject_cov": 0.6721991701244814, "identity": 1.0, "score": 321.0, "evalue": 5.56e-110, "gene_start": 1, "gene_end": 162, "reference_start": 5, "reference_end": 166, "reference_sequence": "MAYKMNNEETLILEKKRYGLEPKITEKEYELLRQNYPIQLIMGYVEYLNTRINLREQVLIPRYETEEMVDIYLKEFAAENQEVLDLCCGSGFIGLAIKKNLRSAKVTLVDISDEAIKQTIENSIVNFGFNHDLKIYQSDLFTNVKGRFDVIISNPPYLNCNDKSLDWKALSFEPKIALCAPEDGWYFYDKILKKYKNYLKKNGWLIMEINPQHIEKWKQIKGAQIKKDINGKYRFVFIQNI" }, "paralog": false, "identity": 0.979253112033195, "score": 469.0, "evalue": 1.08e-170, "description": "Internal stop codon at 878615, 878654, 878711, 878765. Nonsense mutation around 878615, 878654, 878711, 878765.", "psc": { "uniref90_id": "UniRef90_C4XFW9", "gene": "prmC", "product": "peptide chain release factor N(5)-glutamine methyltransferase", "ec_ids": [ "2.1.1.297" ], "uniref50_id": "UniRef50_A0A3P8KWM4", "go_ids": [ "GO:0006412", "GO:0008757", "GO:0018364" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFW9", "EC:2.1.1.297", "UniRef:UniRef50_A0A3P8KWM4", "GO:0006412", "GO:0008757", "GO:0018364" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KWM4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KWM4" ], "product": "peptide chain release factor N(5)-glutamine methyltransferase" } }, "id": "OAIKOHHJFB_1580", "locus": "GCA000209735_01576" }, { "type": "cds", "sequence": "contig_1", "start": 879253, "stop": 880368, "strand": "-", "frame": 2, "gene": "prfA", "product": "peptide chain release factor 1", "db_xrefs": [ "GO:0006415", "GO:0016149", "KEGG:K02835", "SO:0001217", "UniRef:UniRef50_A5IZH6", "UniRef:UniRef90_C4XFX0" ], "nt": "ATGCTTTTTAAAAATGAATATTTTTATATAATTATAAACATGGAAAAAAATATGTTCAATTCGTTGTCTGATATTAAAAATACCTATGACAATTTAGTTAAAAAATTGGATGATCATGAAGTTATCAATAACATCAAGGAATACACAAAAATAAGTAAAGAAGTCAACAAAATTAAGGATATAGCAGAAACCTTTACTAAGTACTTAAATTTACAAAATGACTACAATGATGCAAAAGTAATGATCAATTCAAAAAATGAAGAGGAAGTTCTTTTTGCTAAAAGTATCATTGAAGAAAATGAAGTTAAATTAGCACAACTTGAAGAAGAATTAAAAATTTTAATTCTGCCTAAAGATGAAAACGATGACAAGAATGTTATTATGGAAATTAGAGGTGCAGCAGGCGGTGACGAAGCTAATATTTTTGCTGGCGACTTATTCAAAATGTACACAAAATTTGCAGAAGATTTAAATTTCAAATTAAAAGTTATATCTTCTTCAGCAGCTAGTGTTGGTGGTTTTACTCAAATAGTATTTAGTATTAAGGGTGATAAAGCTTATTCAAAACTTAAGTTTGAAAGTGGAGTGCATCGTGTGCAACGCGTACCTGTAACTGAAAGTGCAGGTAGAATTCATACTTCAACAGCAACAGTTACTGTTATGCCTGAAATTGATGATAGTGTTGATATTGAAATCAAACCACAAGATTTACAAATTGATACATATAGATCATCTGGAGCTGGTGGCCAATCAGTTAATACAACAGACTCAGCGGTTAGAATTACACACTTGCCTACTGGTATAGTTGTTTCATCGCAAGATGAAAGAAGTCAAATAGCAAATAGAGAAACTGCTTTAACAGTTCTTAAAAGTAAGCTTTATGATTTAGAAATTCAAAAGAAACAACAAGAAGAAGCAGGGTATAGAAAACTAGCAGGTCATGGTGATCGGAGCGAAAAAATTAGAACATATAACTATCCACAAGATAGAGTTACAGACCATAGAATTGGATATTCAACAAGTTTAAAACAAGTAATGGAAGGTAAATTATTAACTATTATTGAAGCATTACTAACAGAAGAACAAAATCAAAAAATAAAGGAAAGTGGCTTATAA", "aa": "MLFKNEYFYIIINMEKNMFNSLSDIKNTYDNLVKKLDDHEVINNIKEYTKISKEVNKIKDIAETFTKYLNLQNDYNDAKVMINSKNEEEVLFAKSIIEENEVKLAQLEEELKILILPKDENDDKNVIMEIRGAAGGDEANIFAGDLFKMYTKFAEDLNFKLKVISSSAASVGGFTQIVFSIKGDKAYSKLKFESGVHRVQRVPVTESAGRIHTSTATVTVMPEIDDSVDIEIKPQDLQIDTYRSSGAGGQSVNTTDSAVRITHLPTGIVVSSQDERSQIANRETALTVLKSKLYDLEIQKKQQEEAGYRKLAGHGDRSEKIRTYNYPQDRVTDHRIGYSTSLKQVMEGKLLTIIEALLTEEQNQKIKESGL", "aa_hexdigest": "ca45339f46f8993cc611d5a44d19a4dc", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XFX0", "query_cov": 1.0, "subject_cov": 0.9946380697050938, "identity": 1.0, "score": 692.0, "evalue": 4.14e-251, "valid": true, "gene": "prfA", "product": "peptide chain release factor 1", "uniref50_id": "UniRef50_A5IZH6", "kegg_orthology_id": "K02835", "go_ids": [ "GO:0006415", "GO:0016149" ] }, "pscc": { "uniref50_id": "UniRef50_A5IZH6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IZH6" ], "product": "Peptide chain release factor 1" }, "genes": [ "prfA" ], "id": "OAIKOHHJFB_1581", "locus": "GCA000209735_01577" }, { "type": "cds", "sequence": "contig_1", "start": 880549, "stop": 881529, "strand": "+", "frame": 1, "gene": null, "product": "Ribose-phosphate diphosphokinase", "db_xrefs": [ "EC:2.7.6.1", "KEGG:K00948", "RefSeq:WP_041594256.1", "SO:0001217", "UniParc:UPI000302C729", "UniRef:UniRef100_C4XFX1", "UniRef:UniRef50_Q98R83", "UniRef:UniRef90_A0A3P8K8X3" ], "nt": "ATGGATAAAAAAAATGTAATGCTTTTTGGTATGCCGAATAGCTTGAAATTAGCAGAAAAAATTTCAAAAATTATTAAAGTTCCTTTGACAGGAATTACTAAAACAACTTTTGCTGATGGCGAAGTTATGTTAGTTAGCGACAAAACAGTTAGAGAAAAAGATGTTTTCGTTATTGCTTCAACATCAAGACCAGTTAATGAAAACTTAATGGAGTTATTCTTATTTATTGACTCGCTTAAAAGAGCAAGTGTTAAAAGTGTTACAGTTGCTTTAAGTTACTATGGATATGCACGTCAAGATCACAAAGCAAGTGGACGTCAACCTATTGGTGCAAAACTAGTAGCAGACTTATTACAAACAGCGGGTGCTGACAAAGTTATTGCAGTTGATTTACATAACCCTGCAATTCAAGGTTTCTTCAATATTCCTATTGATGATTTGCGTGGTGCTTATACTTTAGCAAAAGCTATTAAAAATACACATGAATCATTTACTGTTGTTTCACCTGACCACGGTGGAACAATTAGAGCAAGAAAACTTGCTGAATTAATTTCAAACGATGTAAAAATTTGTATTATTGACAAACGTCGTATTGGCACAAACCAAACTGAAGTTTTAGGACTTATTGGTGATATTGAAGGTCAAAATGCAGTTATCATTGATGATATCATTGATACTGGAGGAACAATTTTAAAAGCAGTTGATACATTAAAAGCACACGGTGCAAAGAAAATTATTGTAGCTGCAACTCACGGTATTTTTACAAAAGGATTTGAAATTTTTGAAAATAATCCAAATGTTTATAAAGTAATTATTACTGATTCAATTGAAAATGCAGAATTAAGAGATGGTAAATTCAAAAAACTCGAAGTAATAAGTCTTGACAGATTCCTAGCTAATGTTATTGAATCATCAATTAGTGGTAAATCTATTTCTCATTTATATCAACAATTTGCAAAAGAAATTACTTCAAATAAATAA", "aa": "MDKKNVMLFGMPNSLKLAEKISKIIKVPLTGITKTTFADGEVMLVSDKTVREKDVFVIASTSRPVNENLMELFLFIDSLKRASVKSVTVALSYYGYARQDHKASGRQPIGAKLVADLLQTAGADKVIAVDLHNPAIQGFFNIPIDDLRGAYTLAKAIKNTHESFTVVSPDHGGTIRARKLAELISNDVKICIIDKRRIGTNQTEVLGLIGDIEGQNAVIIDDIIDTGGTILKAVDTLKAHGAKKIIVAATHGIFTKGFEIFENNPNVYKVIITDSIENAELRDGKFKKLEVISLDRFLANVIESSISGKSISHLYQQFAKEITSNK", "aa_hexdigest": "519930c02f742a67f89268afaa1eec3d", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "ups": { "uniparc_id": "UPI000302C729", "ncbi_nrp_id": "WP_041594256.1", "uniref100_id": "UniRef100_C4XFX1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000302C729", "RefSeq:WP_041594256.1", "UniRef:UniRef100_C4XFX1" ] }, "ips": { "uniref100_id": "UniRef100_C4XFX1", "uniref90_id": "UniRef90_A0A3P8K8X3" }, "psc": { "uniref90_id": "UniRef90_A0A3P8K8X3", "product": "Ribose-phosphate diphosphokinase", "ec_ids": [ "2.7.6.1" ], "uniref50_id": "UniRef50_Q98R83", "kegg_orthology_id": "K00948" }, "pscc": { "uniref50_id": "UniRef50_Q98R83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98R83" ], "product": "Ribose-phosphate pyrophosphokinase" }, "genes": [], "id": "OAIKOHHJFB_1582", "locus": "GCA000209735_01578" }, { "type": "cds", "sequence": "contig_1", "start": 881529, "stop": 882188, "strand": "+", "frame": 3, "gene": "rsmG", "product": "16S rRNA (guanine(527)-N(7))-methyltransferase RsmG", "db_xrefs": [ "EC:2.1.1.170", "GO:0008168", "GO:0031167", "GO:0046118", "KEGG:K03501", "SO:0001217", "UniRef:UniRef50_D3VPP6", "UniRef:UniRef90_C4XFX2" ], "nt": "ATGAGCTATAGAAAAATTACTAAAAAAGATTTACTTGAAAAATATTCTTCAAAAATTAGCATATTAAAAGAATATGCTCAAATGATTTATGATCAAAATCAAGTTATGAATATCACAGGTTTTAAAACACTAGATGATATTTTTGAACAAGGTATACTTGATTCTATTTTAGCTTTTGAAAATTACATGATGCATTATGATGAAAATCTAGAAAACAAAAAAATATTAGATGTAGGTGCCGGTGCAGGATTTCCTTCAATTCCAATTCTAATTGCATTAGATAATAAGTTCAATTTAACTATTATTGAATCTCAAAAGAAAAGATGCAATTTTTTAGAAAAAGTTAAAGCACGATTTAATTTAAGTGTCAATATAATTAATTCAAGAGTTGAAGAGTTTTTTAAATTAACAAATTACTTTGAGATTATTACTGCTCGAGCTGTTGCAAGCATTAAAGTTTTATATTTAAGTGCAGCGCACTTATTAAAAAACAGAGGAAAATTTGTTTTATTAAAAGGCGAAAACTTTAGAGATGAGCTTACTGATATTGTAAATATTGCTAAATACATGGGCGAAATGGGCGGCTTTGAATATGATAATGTTTTAAATGAAACGTCTAGCGTGGTGGTTATTAACAAAGGAAAAATTCCTGATGATTGA", "aa": "MSYRKITKKDLLEKYSSKISILKEYAQMIYDQNQVMNITGFKTLDDIFEQGILDSILAFENYMMHYDENLENKKILDVGAGAGFPSIPILIALDNKFNLTIIESQKKRCNFLEKVKARFNLSVNIINSRVEEFFKLTNYFEIITARAVASIKVLYLSAAHLLKNRGKFVLLKGENFRDELTDIVNIAKYMGEMGGFEYDNVLNETSSVVVINKGKIPDD", "aa_hexdigest": "8aa655d6e513edef9385f9978c75a92d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XFX2", "query_cov": 1.0, "subject_cov": 0.948051948051948, "identity": 1.0, "score": 425.0, "evalue": 4.05e-150, "valid": true, "gene": "rsmG", "product": "16S rRNA (guanine(527)-N(7))-methyltransferase RsmG", "ec_ids": [ "2.1.1.170" ], "uniref50_id": "UniRef50_D3VPP6", "kegg_orthology_id": "K03501", "go_ids": [ "GO:0008168", "GO:0031167", "GO:0046118" ] }, "pscc": { "uniref50_id": "UniRef50_D3VPP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D3VPP6" ], "product": "Ribosomal RNA small subunit methyltransferase G" }, "genes": [ "rsmG" ], "id": "OAIKOHHJFB_1583", "locus": "GCA000209735_01579" }, { "type": "cds", "sequence": "contig_1", "start": 882663, "stop": 882914, "strand": "-", "frame": 3, "gene": "nusG", "product": "transcription termination/antitermination protein NusG", "db_xrefs": [ "GO:0006355", "KEGG:K02601", "SO:0001217", "UniRef:UniRef50_A0A4Y6I615", "UniRef:UniRef90_C4XFX3" ], "nt": "ATGATTAGTACAGTTTCTGGAAAAGAAGATAAGGTTGTTGAAGCTTTAAAAAATAGAATTATTAGTGAACAAGTTGAAGATTGCTTTAATCATGAAGCAACACCTGAAGGCGCTTTTAAAATTTTCAAAAAACCAACCTTAACACCTAAAGAATTAGAAAAACAAAAAAATGGTGAAGAATACAAAGTTAAATATGTAAACATGTATTCAGGCTACATTTTTATTAATATGGACATGACAGATAGAGCTTGA", "aa": "MISTVSGKEDKVVEALKNRIISEQVEDCFNHEATPEGAFKIFKKPTLTPKELEKQKNGEEYKVKYVNMYSGYIFINMDMTDRA", "aa_hexdigest": "ed73095dc91506e0802b5751155f2adc", "start_type": "ATG", "rbs_motif": null, "genes": [ "nusG" ], "truncated": "both", "pseudogene": { "start": 882360, "stop": 882950, "observations": { "mutation": [ 882665, 882920 ], "stop": [ 882665, 882920 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFX3", "query_cov": 1.0, "subject_cov": 0.40487804878048783, "identity": 1.0, "score": 168.0, "evalue": 1.66e-51, "gene_start": 1, "gene_end": 83, "reference_start": 13, "reference_end": 95, "reference_sequence": "MEDIMDKNFQWYMISTVSGKEDKVVEALKNRIISEQVEDCFNHEATPEGAFKIFKKPTLTPKELEKQKNGEEYKVKYVNMYSGYIFINMDMTDRAWFIVRNTQYVTGLIGSSGRGAKPTPVSQHQIDKSFKKEIKLQEDFDKGVTHTKFQIGEIVEIIDGPFKGEQGRVLECSEANKKVTVEIEYLGKKTPADFEYKEIKSQEYE" }, "paralog": false, "identity": 0.9846938775510204, "score": 388.0, "evalue": 1.34e-140, "description": "Internal stop codon at 882665, 882920. Nonsense mutation around 882665, 882920.", "psc": { "uniref90_id": "UniRef90_C4XFX3", "gene": "nusG", "product": "transcription termination/antitermination protein NusG", "uniref50_id": "UniRef50_A0A4Y6I615", "kegg_orthology_id": "K02601", "go_ids": [ "GO:0006355" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFX3", "UniRef:UniRef50_A0A4Y6I615", "KEGG:K02601", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_A0A4Y6I615", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4Y6I615" ], "product": "Transcription termination/antitermination protein NusG" } }, "id": "OAIKOHHJFB_1584", "locus": "GCA000209735_01580" }, { "type": "cds", "sequence": "contig_1", "start": 883401, "stop": 883901, "strand": "-", "frame": 3, "gene": null, "product": "Sigma-70 family RNA polymerase sigma factor", "db_xrefs": [ "RefSeq:WP_258408938.1", "SO:0001217", "UniParc:UPI0003193C7E", "UniRef:UniRef100_UPI000F02DED5", "UniRef:UniRef50_C4XFX6", "UniRef:UniRef90_C4XFX6" ], "nt": "ATGATATTGGATTATTTTTACAAAAATGAATTTATCAAAAATATCAAAAGCACTTTAATTAGATTTCCTTATATTCCATTAGAAGCTGAAGATATTTATATTGAATTTTTGCAAACCTATTTAGAAGAAGTTAAAAAGTATAATTCAACTGACAAAAATGTTAAATTTTTAAATTTCTTTTTAAATATCTCCAAATTTTACACATTAAATAAAATTAGATATTGGCTTAGAAAAAAAAGAATTCATAACTCACTTATGACTTCAACTGATGAATTGTTATATGTTTTAGATGAGCATTCTGAAGAACAAATTGAACAAAGAATTAATCAAATTGATACTGAAAACTTTTATCATTTATTAACTGACAAAGATAAAAATATAATTAAAATATTGCAAAATAGTATCAATCAAAAGGACAAATTGATTACACCTAGTAAACTAAAAGAATTTAAAACTAAATTTTTAACTAAATTTAATAATTATTTTCATTTTGCCCATTAA", "aa": "MILDYFYKNEFIKNIKSTLIRFPYIPLEAEDIYIEFLQTYLEEVKKYNSTDKNVKFLNFFLNISKFYTLNKIRYWLRKKRIHNSLMTSTDELLYVLDEHSEEQIEQRINQIDTENFYHLLTDKDKNIIKILQNSINQKDKLITPSKLKEFKTKFLTKFNNYFHFAH", "aa_hexdigest": "19a37b0c524f70322926c32e7d4de0e2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0003193C7E", "ncbi_nrp_id": "WP_258408938.1", "uniref100_id": "UniRef100_UPI000F02DED5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003193C7E", "RefSeq:WP_258408938.1", "UniRef:UniRef100_UPI000F02DED5" ] }, "ips": { "uniref100_id": "UniRef100_UPI000F02DED5", "uniref90_id": "UniRef90_C4XFX6" }, "psc": { "uniref90_id": "UniRef90_C4XFX6", "product": "Sigma-70 family RNA polymerase sigma factor", "uniref50_id": "UniRef50_C4XFX6" }, "pscc": { "uniref50_id": "UniRef50_C4XFX6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFX6" ], "product": "Sigma-70 family RNA polymerase sigma factor" }, "genes": [], "id": "OAIKOHHJFB_1585", "locus": "GCA000209735_01581" }, { "type": "cds", "sequence": "contig_1", "start": 884224, "stop": 884691, "strand": "-", "frame": 2, "gene": null, "product": "RNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0011424F0F" ], "nt": "ATGGAAAAATTATATGTGTGTGGTAGAAATTCAGTTGAAGATGCTTTAAAAGTAAACTTGCCAATTGAAACAATTTATGTCAACTCATTTCAACTCGCACAAAAATTAAAACAAATTACCAAAGCAAAAATAGAAATGAAGGATAATTCATTTTTTGCTAACTATCGTAATGATAACCATCAAGGTGTCATTGCTATTTTAAGAGACTTCCCTATTTATGATCTTGAAACAATTAAAAAAGATAATCCTGAAAATGTTTTAGTTTTAGACCACATTCAAGACCCTCATAATTTAGGTGCAATCATTAGAACTGCTAATACTTGCGGAATTAAGCATCTTATTATTAGTAAAGAAAGATCAGCTAATATTACTTCAACAGTTTTAAAAGTTAGCTCAGGTGGTTTTGTTAATATGAAAATAATTAAAGTTAATAGTATTAGTGCTACCTTAACAAAACTTAAAAAATGA", "aa": "MEKLYVCGRNSVEDALKVNLPIETIYVNSFQLAQKLKQITKAKIEMKDNSFFANYRNDNHQGVIAILRDFPIYDLETIKKDNPENVLVLDHIQDPHNLGAIIRTANTCGIKHLIISKERSANITSTVLKVSSGGFVNMKIIKVNSISATLTKLKK", "aa_hexdigest": "441e77b1e4b25f25cf0d3eb7a60482b8", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0011424F0F", "query_cov": 1.0129032258064516, "subject_cov": 0.8440860215053764, "identity": 0.535, "score": 157.0, "evalue": 2.9e-46, "valid": false, "product": "RNA methyltransferase", "uniref50_id": "UniRef50_UPI0011424F0F" }, "pscc": { "uniref50_id": "UniRef50_UPI0011424F0F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0011424F0F" ], "product": "RNA methyltransferase" }, "genes": [], "id": "OAIKOHHJFB_1586", "locus": "GCA000209735_01582" }, { "type": "cds", "sequence": "contig_1", "start": 884815, "stop": 885243, "strand": "-", "frame": 2, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000F036BAC", "UniRef:UniRef90_UPI000F036BAC" ], "nt": "ATGTCAAAATCTTTAGGCAATGTTATTCTTGCAAAAGACTTTTTAAAAGAACATGATCGCGAAATTTTGAAGTTGATTATTTTGAGTTCAAAAATTTCAGCTCCAATTAATGTGACAAATGAATTAATTGAAAATATGTATGTTATTTTGAATAAATACAAAAAATTAATTTTTAAATTCTTTATTAATTTTAAAAATATTGACAAATCAAAAATTAATAAAAATGAAGAATTTATTAAGATAATGAATTCTTTAAAAATAGGCGATTTTTCTGATTTTAATTATTTATTAAATGAACAAATTAAAGAATTCAATAAAACCAAAAATATAAAAAATGCAGAAATAATTTTTAATACTTTAAAAGTTATCCACCCTCAATTAACAATTAAAGCTAACTATAACAAAGCTTTCGAGCTTTTTGACCAATGA", "aa": "MSKSLGNVILAKDFLKEHDREILKLIILSSKISAPINVTNELIENMYVILNKYKKLIFKFFINFKNIDKSKINKNEEFIKIMNSLKIGDFSDFNYLLNEQIKEFNKTKNIKNAEIIFNTLKVIHPQLTIKANYNKAFELFDQ", "aa_hexdigest": "09abe47dc6b1cdc56047565e701605be", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "both", "pseudogene": { "start": 884737, "stop": 885543, "observations": { "mutation": [ 884817, 885282, 885432, 885492 ], "stop": [ 884817, 885282, 885432, 885492 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000F036BAC", "query_cov": 1.0, "subject_cov": 0.4369230769230769, "identity": 1.0, "score": 272.0, "evalue": 1.17e-89, "gene_start": 1, "gene_end": 142, "reference_start": 158, "reference_end": 299, "reference_sequence": "LSHLEKVTDNMGLIIEYVNKQVKNNNAYIDQEGNVWFDVKKFENHYGQVANLNLDKMIFEEQNNNKKFEADFALWKKTNVGIQFDSPFSKGRPGWHTECAVLINKHFKNKGLDIHGGGMDLTFPHHENENIQHFALYNQDITKEWIRCGQINYEGEKMSKSLGNVILAKDFLKEHDREILKLIILSSKISAPINVTNELIENMYVILNKYKKLIFKFFINFKNIDKSKINKNEEFIKIMNSLKIGDFSDFNYLLNEQIKEFNKTKNIKNAEIIFNTLKVIHPQLTIKANYNKAFELFDQWRCFIDKKEYKQADKIRKKLMKLGVY" }, "paralog": false, "identity": 0.9850746268656716, "score": 528.0, "evalue": 5.45e-193, "description": "Internal stop codon at 884817, 885282, 885432, 885492. Nonsense mutation around 884817, 885282, 885432, 885492.", "psc": { "uniref90_id": "UniRef90_UPI000F036BAC", "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_UPI000F036BAC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000F036BAC", "UniRef:UniRef50_UPI000F036BAC" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000F036BAC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000F036BAC" ], "product": "class I tRNA ligase family protein" } }, "id": "OAIKOHHJFB_1587", "locus": "GCA000209735_01583" }, { "type": "cds", "sequence": "contig_1", "start": 885607, "stop": 885972, "strand": "-", "frame": 2, "gene": null, "product": "Cysteine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZN9" ], "nt": "ATGTTAAAAATTTATGTCTGCGGTCCAACTGTTTATAATGATCTACATATTGGAAATATGGTTCCAATATTAACTTTTGATTTAATGCTAAAAGCTAGACGAGTTTTAGGAGAAAAATTCACTTTTGTTCACAATATTACTGATATTGATGACAAAATAATCAATCGTGCTAGAGAGCTAAATATAAGCGAAAAAGAATTAAGTCAAAAATATACTAAAGACTACTTGAAATTATTAAAAACCTTGGATGTTGACACTATAAGTCATCTTGAAAAAGTTACTGACAATATGGGCTTAATTATTGAATATGTTAATAAGCAAGTTAAAAATAATAATGCTTATATTGACCAAGAAGGTAATGTTTGA", "aa": "MLKIYVCGPTVYNDLHIGNMVPILTFDLMLKARRVLGEKFTFVHNITDIDDKIINRARELNISEKELSQKYTKDYLKLLKTLDVDTISHLEKVTDNMGLIIEYVNKQVKNNNAYIDQEGNV", "aa_hexdigest": "37afea71354c967a9ddd4b884bac9645", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A448ZZN9", "query_cov": 0.9421487603305785, "subject_cov": 0.8571428571428571, "identity": 0.5, "score": 123.0, "evalue": 5.18e-34, "valid": false, "product": "Cysteine--tRNA ligase", "uniref50_id": "UniRef50_A0A448ZZN9" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZN9" ], "product": "Cysteine--tRNA ligase" }, "genes": [], "id": "OAIKOHHJFB_1588", "locus": "GCA000209735_01584" }, { "type": "cds", "sequence": "contig_1", "start": 886047, "stop": 887048, "strand": "+", "frame": 3, "gene": null, "product": "arginine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.19", "KEGG:K01887", "SO:0001217", "UniRef:UniRef50_A0A3P8KM35", "UniRef:UniRef90_C4XFX9" ], "nt": "ATGGTTATAGTTCATAAATTGAACAAGTTAATTAAAAACATAGTAAATGAACTAAAAGCTGAAAACAAGATTTTCAGTTCTTTTAAGTTTCTTGATGGTGAAAAGAAGTTTTCACTAAAAATGGCTCGTAAAACTAAGGGCCATAACAATAATATTAACCATATTGAATGCGATATTGTTTTTCAACTTAAAAAATTTAGTTTTATTCCTTATATTGAATTAGCAGAAGAAATTAAAAAGAGATTAATCAAAGATTCAATGGTTAAAGATGTGACAATTGATCGTTATGGGCTTTTACATATTGTTTTGAATGAAAAAGATTTAGTTTCAGTGGTTAATGAAGTTATTAAGAAAGCTTATAAATATGGACAAGAAAAGAAAAATAATATTAAAGTTAATATTGAATATACTTCAGCTTCGCCACTTAATTCTTTAACTTTGATGAATGCTAGAAATGCTTTAGTTGGAGAAACTATAGCAAGAATTTTAGAATACTTAGGTTATCAAGTTACTCGAGAATTTTTAGTTTATCCAGGTTTAAAGAAAATGGAAAAAGTAAGCCAAATTGTTCATGAACAATATGTTAACTTGTTTGAATTAAATGACAATTCTATTAAAGTTGACAAGGATATTTTAGAATGTGCAAAGAATATTAAAGCACAAAATGGTGATCTTTATTTAGACATAAATGACACAATTAAAAGAAATAAATTTGATTTGGCAATTGTTTTTTATTTCTTAAATAAATCCAAAAAACAACTTAAAAATTTAGGTGTCTTTTTTGACATAGTGAGCTATTTTGAAGACATTTTGGTCAAAGAAAAGATTGACAAAGTATTAGAATTATTGTCACCTTTTATTTATGATAATGATTCAAATTTAATACTTAATACAAATCTTGATGATAGTAAAGAACGCATTCTTGTTCATAGAAATGGCACGAGTTCATATTTATTATTAGACATAATTTATCATCTTGAAAAATTAAAAAGAGCAGACTGA", "aa": "MVIVHKLNKLIKNIVNELKAENKIFSSFKFLDGEKKFSLKMARKTKGHNNNINHIECDIVFQLKKFSFIPYIELAEEIKKRLIKDSMVKDVTIDRYGLLHIVLNEKDLVSVVNEVIKKAYKYGQEKKNNIKVNIEYTSASPLNSLTLMNARNALVGETIARILEYLGYQVTREFLVYPGLKKMEKVSQIVHEQYVNLFELNDNSIKVDKDILECAKNIKAQNGDLYLDINDTIKRNKFDLAIVFYFLNKSKKQLKNLGVFFDIVSYFEDILVKEKIDKVLELLSPFIYDNDSNLILNTNLDDSKERILVHRNGTSSYLLLDIIYHLEKLKRAD", "aa_hexdigest": "5e548d637e71a0b64bdabac03ce65b5f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 886017, "stop": 887351, "observations": { "mutation": [ 887046 ], "stop": [ 887046 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFX9", "query_cov": 1.0, "subject_cov": 0.6021699819168174, "identity": 1.0, "score": 641.0, "evalue": 1.15e-228, "gene_start": 1, "gene_end": 333, "reference_start": 11, "reference_end": 343, "reference_sequence": "MYLKIYSGSNMVIVHKLNKLIKNIVNELKAENKIFSSFKFLDGEKKFSLKMARKTKGHNNNINHIECDIVFQLKKFSFIPYIELAEEIKKRLIKDSMVKDVTIDRYGLLHIVLNEKDLVSVVNEVIKKAYKYGQEKKNNIKVNIEYTSASPLNSLTLMNARNALVGETIARILEYLGYQVTREFLVYPGLKKMEKVSQIVHEQYVNLFELNDNSIKVDKDILECAKNIKAQNGDLYLDINDTIKRNKFDLAIVFYFLNKSKKQLKNLGVFFDIVSYFEDILVKEKIDKVLELLSPFIYDNDSNLILNTNLDDSKERILVHRNGTSSYLLLDIIYHLEKLKRADWIIDILDANHTQYSHSLISALKFLNAYNNNIDIAFCENFQIKENNEVKSMTEKNQNVIFIDELVKKVNLNEIKFFFLEKSTKHQITIEKEILNQKKISHNFDFILNTYNKINKLLDESKNNKCYCNKMNQNNFLSKLILRLDYFPYVLRKVIKTLNPFYLTKYLKEMCYLINQNLKDKLKKDYRNFYPILEAVKNVFDNGLNLLGIDFKN" }, "paralog": false, "identity": 0.9954954954954955, "score": 865.0, "evalue": 3.99e-319, "description": "Internal stop codon at 887046. Nonsense mutation around 887046.", "psc": { "uniref90_id": "UniRef90_C4XFX9", "product": "arginine--tRNA ligase", "ec_ids": [ "6.1.1.19" ], "uniref50_id": "UniRef50_A0A3P8KM35", "kegg_orthology_id": "K01887", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFX9", "EC:6.1.1.19", "UniRef:UniRef50_A0A3P8KM35", "KEGG:K01887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KM35", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KM35" ], "product": "arginine--tRNA ligase" } }, "id": "OAIKOHHJFB_1589", "locus": "GCA000209735_01585" }, { "type": "cds", "sequence": "contig_1", "start": 887193, "stop": 887678, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05746.21" ], "nt": "ATGACAGAAAAAAACCAAAACGTCATTTTTATAGATGAATTAGTAAAAAAAGTTAATTTAAATGAAATCAAATTTTTCTTTTTAGAAAAGAGTACTAAACATCAAATAACAATTGAAAAGGAAATTTTAAATCAAAAGAAAATTAGTCATAATTTCGATTTCATATTAAATACTTATAATAAAATAAATAAATTATTAGATGAATCAAAAAACAATAAATGTTATTGTAACAAAATGAATCAAAACAATTTTTTAAGCAAACTTATTTTAAGGCTTGATTATTTTCCTTATGTTTTAAGAAAAGTAATCAAAACTTTAAATCCTTTTTACTTAACAAAATATCTAAAAGAAATGTGTTATTTGATAAATCAAAATCTAAAAGATAAACTTAAAAAAGATTATAGAAACTTCTATCCAATTTTAGAAGCTGTCAAAAATGTATTTGATAATGGACTAAATTTACTTGGAATAGATTTTAAAAATTAA", "aa": "MTEKNQNVIFIDELVKKVNLNEIKFFFLEKSTKHQITIEKEILNQKKISHNFDFILNTYNKINKLLDESKNNKCYCNKMNQNNFLSKLILRLDYFPYVLRKVIKTLNPFYLTKYLKEMCYLINQNLKDKLKKDYRNFYPILEAVKNVFDNGLNLLGIDFKN", "aa_hexdigest": "519a6e33dd9a44f05299dbec9cc1006f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05746.21", "name": "DALR_1", "length": 109, "aa_cov": 0.6273291925465838, "hmm_cov": 1.0, "evalue": 3.01026017480981e-09, "score": 33.0975456237793, "start": 59, "stop": 159 } ], "seq_stats": { "molecular_weight": 19449.599899999997, "isoelectric_point": 9.47048625946045 }, "id": "OAIKOHHJFB_1590", "locus": "GCA000209735_01586" }, { "type": "cds", "sequence": "contig_1", "start": 888263, "stop": 888814, "strand": "+", "frame": 2, "gene": null, "product": "EVE domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFY0", "UniRef:UniRef90_C4XFY0" ], "nt": "ATGAAAAAACGTAGTCAATCATCATATGTTGACCTTAACGCTATCAAAGCATCTCTTGAACTTAAAATTAAAGAACTTGAAGACCAAAAAAATGCTTCAGATGAAACAATCAATAGCTTAAAAGAACAATTAGAAAACAAAAAATTCGACTTCATGAAAGTTGAAGAAGAAATTGAAGCTATTAAAAACCAAAAAGATCAAGAAATCAACGCAGTACGTGAAGAAGCTGAAAAGAAAATTGAAGAAGTTAAAGCAGAAGCAGCTGCAACAGCAGAACAACCAGCTTATGATCCAAATGCTGAAGTTCAATACGATCCATATGGAAACCCAATTGATCTAAATGCACAGCCAGCAATGGACCCTTATGGAAATCCATATGTACAAAGTGAAACAATTATGGCAGATCCTTATGGAACAGAAGGCGGATATTTAGTACAACCTGAAGCCTATGGGCAATTAACTCCATATGAAGCTAACTATGGATACCCAACAGGTATGTATGATGGTACTCAATTCTACCCTGTTCAACGAAAATCAGACAAAGGAATTTGA", "aa": "MKKRSQSSYVDLNAIKASLELKIKELEDQKNASDETINSLKEQLENKKFDFMKVEEEIEAIKNQKDQEINAVREEAEKKIEEVKAEAAATAEQPAYDPNAEVQYDPYGNPIDLNAQPAMDPYGNPYVQSETIMADPYGTEGGYLVQPEAYGQLTPYEANYGYPTGMYDGTQFYPVQRKSDKGI", "aa_hexdigest": "90f9bd790fb7a7f1256243aa94c1a3e4", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [], "truncated": "both", "pseudogene": { "start": 887963, "stop": 888910, "observations": { "mutation": [ 887990, 888812 ], "stop": [ 887990, 888812 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFY0", "query_cov": 1.0, "subject_cov": 0.5111731843575419, "identity": 1.0, "score": 265.0, "evalue": 6.4e-86, "gene_start": 1, "gene_end": 183, "reference_start": 144, "reference_end": 326, "reference_sequence": "MMKRLNLLWKPKEDETYPWLLKHPKIKEGLAKFKTRQDAVDWFLNFGDEVYIWFQNNEGIFGGQLAVLESENPKDKDSKKLLSIPKVSGFDGGETYNGICEEFSIHPSYLSREPYKSALAKRIPEIDFVLISDPATYFPADLEMKKRSQSSYVDLNAIKASLELKIKELEDQKNASDETINSLKEQLENKKFDFMKVEEEIEAIKNQKDQEINAVREEAEKKIEEVKAEAAATAEQPAYDPNAEVQYDPYGNPIDLNAQPAMDPYGNPYVQSETIMADPYGTEGGYLVQPEAYGQLTPYEANYGYPTGMYDGTQFYPVQRKSDKGIWVGVGIAAAVLLIILILDILVLLSLTNVAQIF" }, "paralog": false, "identity": 0.9809523809523809, "score": 513.0, "evalue": 5.38e-186, "description": "Internal stop codon at 887990, 888812. Nonsense mutation around 887990, 888812.", "psc": { "uniref90_id": "UniRef90_C4XFY0", "product": "EVE domain-containing protein", "uniref50_id": "UniRef50_C4XFY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFY0", "UniRef:UniRef50_C4XFY0" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFY0" ], "product": "EVE domain-containing protein" } }, "id": "OAIKOHHJFB_1591", "locus": "GCA000209735_01587" }, { "type": "cds", "sequence": "contig_1", "start": 889038, "stop": 889217, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACAAATACAAATTTTTATTACAGAAAAGAGGAAATATGAAAATTATTGTCATTGGTGCAAACCATGCAGGGACCAGCTTTTTAAGAACATTAAAAACTGTAAATCCTAAAGCAGAAATTACAGCTTATGACCGTAATACAAACACATCATTTTTAGGTTGTGGTATTGCAATTTGA", "aa": "MYKYKFLLQKRGNMKIIVIGANHAGTSFLRTLKTVNPKAEITAYDRNTNTSFLGCGIAI", "aa_hexdigest": "0f86ef6916333231590c079f92902330", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6593.6998, "isoelectric_point": 10.104017066955567 }, "id": "OAIKOHHJFB_1592", "locus": "GCA000209735_01588" }, { "type": "cds", "sequence": "contig_1", "start": 889272, "stop": 889421, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAAAAGAATATGGAGTAAATTTAAAAACTTCACATGAAGTTATCGAAATTGATAGAAAGAAAAAAGAAGTTACAGTTCAAGATTTAGATAGTGGTAAAACCTTTAAAGACACATATGATAAATTGGTTTTTGCAGGTGGTACATGA", "aa": "MQKEYGVNLKTSHEVIEIDRKKKEVTVQDLDSGKTFKDTYDKLVFAGGT", "aa_hexdigest": "41c581299c451c79791b6a3c1ee58e33", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5591.2629000000015, "isoelectric_point": 6.544634819030762 }, "id": "OAIKOHHJFB_1593", "locus": "GCA000209735_01589" }, { "type": "cds", "sequence": "contig_1", "start": 889668, "stop": 890267, "strand": "+", "frame": 3, "gene": null, "product": "NADH oxidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5P1", "UniRef:UniRef90_C4XFY1" ], "nt": "ATGCAAAAAAACATGAAAAAAGAAGGTGTTAAACTTGCTCTTGGAGAAAAAGTTGTTGAATTCAAATCAAAAGATGGAAAACATGTATCACAAGTTATTACAGATAAAAGTAAATATGATGCAGATCTTGTAATTATGTCAGTAGGTTTCAAACCTAGAACATATGTATTGAATGATGTTGAAAAATTACCTAATGGAGCAATTAAAGTTAATGAATTCCAACAATCAATTTCTGACGAAAACATCTATGTTTTAGGTGATTCTGCAGCATTAAAACACTGTGTAAGCAACACATATGAACACGTAGCTTTAGCAACTAATGCAGTTAAAACAGGTATTGTAGCTGCATTAAATTTAGCTGGACTTAAAGTGCCATTTCCAGGAGTAGTAGGAACAAATGCTATTAATGTTTTTGGTTGTCACTATGCTGCAACAGGATTTAATGAAGATGCAGCTAGAGCTCATGGATTTGATGTAGCTAGTGAATATTTTGAAGACAATGACCGCTGCGAATTTGCTGGTAAAAGTGAAAAAGTTGGATGCAAAATTATTTTTGACAAGAAAACATTACAAATTTTAGGTGTTCAAATCGGATCATGA", "aa": "MQKNMKKEGVKLALGEKVVEFKSKDGKHVSQVITDKSKYDADLVIMSVGFKPRTYVLNDVEKLPNGAIKVNEFQQSISDENIYVLGDSAALKHCVSNTYEHVALATNAVKTGIVAALNLAGLKVPFPGVVGTNAINVFGCHYAATGFNEDAARAHGFDVASEYFEDNDRCEFAGKSEKVGCKIIFDKKTLQILGVQIGS", "aa_hexdigest": "f32dd76e9dbb953bbcff6925a03c8c50", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 889368, "stop": 890441, "observations": { "mutation": [ 889419, 890265 ], "stop": [ 889419, 890265 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFY1", "query_cov": 1.0, "subject_cov": 0.4216101694915254, "identity": 1.0, "score": 389.0, "evalue": 1.02e-132, "gene_start": 1, "gene_end": 199, "reference_start": 216, "reference_end": 414, "reference_sequence": "MNKIKMYKYKFLLQKRGNMKIIVIGANHAGTSFLRTLKTVNPKAEITAYDRNTNTSFLGCGIAIWVGGKAKQPDGLFYSSPNILQKEYGVNLKTSHEVIEIDRKKKEVTVQDLDSGKTFKDTYDKLVFAGGTWPIEPKIPGIELDNILLSKLYQHAQVLVKKANDKKIQNVVVVGAGYIGIELVEAFHNKGKKVTLIDLADRVVPGYLDGEFTDIMQKNMKKEGVKLALGEKVVEFKSKDGKHVSQVITDKSKYDADLVIMSVGFKPRTYVLNDVEKLPNGAIKVNEFQQSISDENIYVLGDSAALKHCVSNTYEHVALATNAVKTGIVAALNLAGLKVPFPGVVGTNAINVFGCHYAATGFNEDAARAHGFDVASEYFEDNDRCEFAGKSEKVGCKIIFDKKTLQILGVQIGSWGQVNHTEVIYMFGLALQRKLTLPECALTDVFFLPHFNKPFNFFLMPMLKALGLKYKA" }, "paralog": false, "identity": 0.9943977591036415, "score": 706.0, "evalue": 5.19e-260, "description": "Internal stop codon at 889419, 890265. Nonsense mutation around 889419, 890265.", "psc": { "uniref90_id": "UniRef90_C4XFY1", "product": "NADH oxidase", "uniref50_id": "UniRef50_Q4A5P1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFY1", "UniRef:UniRef50_Q4A5P1" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5P1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5P1" ], "product": "NADH oxidase" } }, "id": "OAIKOHHJFB_1594", "locus": "GCA000209735_01590" }, { "type": "cds", "sequence": "contig_1", "start": 890298, "stop": 890441, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGGTTTAGCACTTCAAAGAAAATTGACTCTTCCTGAATGTGCTTTAACTGACGTCTTCTTCTTGCCTCACTTTAACAAACCATTTAATTTCTTCTTAATGCCAATGCTTAAAGCACTTGGACTTAAATATAAAGCTTAA", "aa": "MFGLALQRKLTLPECALTDVFFLPHFNKPFNFFLMPMLKALGLKYKA", "aa_hexdigest": "b0f21f40ec855f6bdf05dc44c1ed6776", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5462.648099999999, "isoelectric_point": 9.746475410461425 }, "id": "OAIKOHHJFB_1595", "locus": "GCA000209735_01591" }, { "type": "cds", "sequence": "contig_1", "start": 890503, "stop": 890709, "strand": "-", "frame": 2, "gene": "rpsT", "product": "30S ribosomal protein S20", "db_xrefs": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "SO:0001217", "UniParc:UPI0001A8C143", "UniRef:UniRef100_C4XFY2", "UniRef:UniRef50_Q98PR3", "UniRef:UniRef90_A0A3P8LHZ8" ], "nt": "ATGAAAACAAGAGTTCGTAAAGCAATTAGAGTAGCTAGAGAAGCAATTTTAGCTAAAGATAAAAACGCTCAAGAGTTAGTTAATAAAGCTCACTCAATTATTGCTACAGCTGTTCAAAAAGGTGTTTTCCACCCAAATAAAGGTGCAAGAAAATCATCTCGTTTAGACAAATTTGTTAACGAACAAAATGCTAAAAAAGATGCATAA", "aa": "MKTRVRKAIRVAREAILAKDKNAQELVNKAHSIIATAVQKGVFHPNKGARKSSRLDKFVNEQNAKKDA", "aa_hexdigest": "67da7e5963be6a976ad5bfd5b8f3a442", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C143", "uniref100_id": "UniRef100_C4XFY2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C143", "UniRef:UniRef100_C4XFY2" ] }, "ips": { "uniref100_id": "UniRef100_C4XFY2", "uniref90_id": "UniRef90_A0A3P8LHZ8" }, "psc": { "uniref90_id": "UniRef90_A0A3P8LHZ8", "gene": "rpsT", "product": "30S ribosomal protein S20", "uniref50_id": "UniRef50_Q98PR3", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PR3" ], "product": "Small ribosomal subunit protein bS20" }, "genes": [ "rpsT" ], "id": "OAIKOHHJFB_1596", "locus": "GCA000209735_01592" }, { "type": "gap", "sequence": "contig_1", "start": 890721, "stop": 890820, "strand": ".", "length": 100, "id": "OAIKOHHJFB_1811" }, { "type": "cds", "sequence": "contig_1", "start": 891292, "stop": 892023, "strand": "+", "frame": 1, "gene": null, "product": "DNA processing protein", "db_xrefs": [ "RefSeq:WP_013527027.1", "SO:0001217", "UniParc:UPI0001F59832", "UniRef:UniRef100_C4XFY3", "UniRef:UniRef50_C4XFY3", "UniRef:UniRef90_C4XFY3" ], "nt": "ATGAATGAATTATTAGTTTATTTAAGTTATAAATATGAAGGGAATATTTTTGAAATTTACAAATTTTTGAAAAATAAGAAAAAAATTAATCAAGCAGAAATAGATATAGTATTAAAGGAGCTAGAAGAAAAAGGTATTAAGTATATAACAGCTTTAGACGAAAATTTTCCAGAGATATTAAAAAATTTTCGTTATTCGCCATATGTTCTTTTTTATAAAGGCAATATTGGCTTATTAAATAAGAAAGCAGTTTGCTTAACTGGAGATATTGAAAGTCCAATTGTGGACATCAATATTAAGAAATCACAAAATACATTTTTGGAGAATTATCCTTTAATAACGAATGATTTCAAGAATTTAGATCAAAAATTTGTTGAAATATATCGTCAAAATAAAAAAGAAATAATCCATGTATTGCCTTATGGATTAGATTATATAAAGGAAACTATAAATTTAGATACAGAATTGTATATTAGTCAATATCCACCACAAACTCATGCTAAATTATTTAGATTTAAAGAAAGGAACATTTTAGTTTCATTTTTAGCTGAATTTTTAATTGTTTATAGCAGCAAAAGCGACTCGGGTATTTTGAGTCTTGCAACAGCTTTTGCTAACAATAATAAGGAAGTGTATTGTTATCCGGGTTTAAGTTATGATGATGGAAACAATTATTTAATTAAATTAGGAGCTAATCTAATTACGCAAATTGCAGAAGTACAATATTATTAA", "aa": "MNELLVYLSYKYEGNIFEIYKFLKNKKKINQAEIDIVLKELEEKGIKYITALDENFPEILKNFRYSPYVLFYKGNIGLLNKKAVCLTGDIESPIVDINIKKSQNTFLENYPLITNDFKNLDQKFVEIYRQNKKEIIHVLPYGLDYIKETINLDTELYISQYPPQTHAKLFRFKERNILVSFLAEFLIVYSSKSDSGILSLATAFANNNKEVYCYPGLSYDDGNNYLIKLGANLITQIAEVQYY", "aa_hexdigest": "ccab20775a6ba2f960cb48801231d001", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001F59832", "ncbi_nrp_id": "WP_013527027.1", "uniref100_id": "UniRef100_C4XFY3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59832", "RefSeq:WP_013527027.1", "UniRef:UniRef100_C4XFY3" ] }, "ips": { "uniref100_id": "UniRef100_C4XFY3", "uniref90_id": "UniRef90_C4XFY3" }, "psc": { "uniref90_id": "UniRef90_C4XFY3", "product": "DNA processing protein", "uniref50_id": "UniRef50_C4XFY3" }, "pscc": { "uniref50_id": "UniRef50_C4XFY3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFY3" ], "product": "DNA processing protein" }, "genes": [], "id": "OAIKOHHJFB_1597", "locus": "GCA000209735_01593" }, { "type": "cds", "sequence": "contig_1", "start": 892020, "stop": 892160, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGTTAAAAAATGCTCACATTGGTTTTGCAATGGGAAATGCCACTGAAGAAGCAAAAGTAGCTGCCGATAGAGTTATAGGTTCTAATTTAACTGGCGCTATTGGTCAAGCAATTAATGAAATTTTAGACGACAAAAATTAA", "aa": "MLKNAHIGFAMGNATEEAKVAADRVIGSNLTGAIGQAINEILDDKN", "aa_hexdigest": "3a66b63781eb15e149a092db13a4386e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 38, "aa_cov": 0.8260869565217391, "hmm_cov": 1.0, "evalue": 7.09068218182132e-09, "score": 31.815629959106445, "start": 1, "stop": 38 } ], "seq_stats": { "molecular_weight": 4783.357, "isoelectric_point": 4.950130653381346 }, "id": "OAIKOHHJFB_1598", "locus": "GCA000209735_01594" }, { "type": "cds", "sequence": "contig_1", "start": 892245, "stop": 892379, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCATTAAGAAATCCATTAATTACTGCAGCAGAAACCACAAAGTATTTTCAAAATAAATTAAAAGATAAACAAAAAGTTTTCTTAACAAACTCAATTTACACTGATGTTAATCCTTTAGGTGTAACTAAGTGA", "aa": "MALRNPLITAAETTKYFQNKLKDKQKVFLTNSIYTDVNPLGVTK", "aa_hexdigest": "abd291e4e154f62124e4ba317619ee8d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5014.794700000002, "isoelectric_point": 9.777162361145017 }, "id": "OAIKOHHJFB_1599", "locus": "GCA000209735_01595" }, { "type": "cds", "sequence": "contig_1", "start": 892518, "stop": 892868, "strand": "-", "frame": 3, "gene": null, "product": "COF family HAD hydrolase protein", "db_xrefs": [ "EC:3.1.3.104", "KEGG:K21064", "SO:0001217", "UniRef:UniRef50_C4XFY4", "UniRef:UniRef90_A0A3P8KLL0" ], "nt": "ATGCAAAACAAAAGAAAAGTGTTTGCTTTTGACTTGGATGGCACATTATTAACAGATGGAGTTAATACTCATCCAGAAACTCAAGAAGCAATTTTAAAATCATATAATTTAGGACATGCAAATATCATTTGTACTGGTCGTGGTTTGCAAAAAACTTTACCACTTTTAAAATCAATTAAAGGCATTAATTATTTTGTATGTTCAAATGGTGCATTATTTTATGATGTTGAAAAAGATTATTATAAAGTTTTAGGCGAAGTTAATAAAGATATATTTAATGATCTTTTTGATTATGCTCAAGCTCATGATTTAATTTTAACTATTGATACTCCTGAATTTAATGGTGCATGA", "aa": "MQNKRKVFAFDLDGTLLTDGVNTHPETQEAILKSYNLGHANIICTGRGLQKTLPLLKSIKGINYFVCSNGALFYDVEKDYYKVLGEVNKDIFNDLFDYAQAHDLILTIDTPEFNGA", "aa_hexdigest": "0a4025e14158ec0fc81e5f56f09c34d2", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 892215, "stop": 892868, "observations": { "mutation": [ 892247, 892484, 892520 ], "stop": [ 892247, 892484, 892520 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8KLL0", "query_cov": 1.0, "subject_cov": 0.4128113879003559, "identity": 0.845, "score": 207.0, "evalue": 4.37e-65, "gene_start": 1, "gene_end": 116, "reference_start": 1, "reference_end": 116, "reference_sequence": "MSKKRIVFAFDLDGTLLTDGVNAHPETQDAIIKSYNQGHANIICTGRGLQKTLPLLKTIKGINYFVCSNGALFYDVEKDYYRVLGEVDKSIFNELFDYANTNNLILTIDTPDFNGSWSKNSDKNILPEWVSKQNKLSDKNPHFLKPLSALTSVLNNPESKITQMAIRNPLDSAQETTKHFQGRLKGKQKVFLTNSIYTDVNPLHTTKWYGLKTLLDYLKIDTHELYCFGDSGNDIEMLQNAHIGYAMGNGTNDAKLAADKIIGSNLTGAIGQVINEILEKN" }, "paralog": false, "identity": 0.7695852534562212, "score": 336.0, "evalue": 2.28e-118, "description": "Internal stop codon at 892247, 892484, 892520. Nonsense mutation around 892247, 892484, 892520.", "psc": { "uniref90_id": "UniRef90_A0A3P8KLL0", "product": "COF family HAD hydrolase protein", "ec_ids": [ "3.1.3.104" ], "uniref50_id": "UniRef50_C4XFY4", "kegg_orthology_id": "K21064", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8KLL0", "EC:3.1.3.104", "UniRef:UniRef50_C4XFY4", "KEGG:K21064" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFY4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFY4" ], "product": "COF family HAD hydrolase protein" } }, "id": "OAIKOHHJFB_1600", "locus": "GCA000209735_01596" }, { "type": "cds", "sequence": "contig_1", "start": 893185, "stop": 893559, "strand": "-", "frame": 2, "gene": "ecfT", "product": "Energy-coupling factor transporter transmembrane protein EcfT", "db_xrefs": [ "KEGG:K16785", "SO:0001217", "UniRef:UniRef50_C4XFY5", "UniRef:UniRef90_C4XFY5" ], "nt": "ATGATTATGGTTACTTCTTTATTATTGATTACAACCAAACCAATACTACTTACTAAAGCACTTGAAGACTTGATGTTTCCATTTAAACTTCTATTCATTCCAACTCACATAATTGCTATGATTATTTCGATTGCATTAAGATTTATTCCTACTCTTTTTGAAGAAGCACAAAGAATAATGAAAGCTCAAGCAAGTCGTGGAGTTGACTTTAAGAATGGAAAAATTAAAGAAAAAGTTAAATCATTTACAACATTAATTATTCCATTGTTTGTTACTTCTTTTGCTAAAGCGGAAGACTTATCAAATGCTATGGAAACTAGGGGTTATGATCCTTATGCAAAAAGAACAAGATATCGTAAATTAAGAATAGGTTGA", "aa": "MIMVTSLLLITTKPILLTKALEDLMFPFKLLFIPTHIIAMIISIALRFIPTLFEEAQRIMKAQASRGVDFKNGKIKEKVKSFTTLIIPLFVTSFAKAEDLSNAMETRGYDPYAKRTRYRKLRIG", "aa_hexdigest": "569452122d6e589593b0f7f8381d720c", "start_type": "ATG", "rbs_motif": null, "genes": [ "ecfT" ], "truncated": "both", "pseudogene": { "start": 893068, "stop": 893859, "observations": { "mutation": [ 893097, 893187, 893637 ], "stop": [ 893097, 893187, 893637 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFY5", "query_cov": 1.0, "subject_cov": 0.4290657439446367, "identity": 1.0, "score": 228.0, "evalue": 2.51e-73, "gene_start": 1, "gene_end": 124, "reference_start": 127, "reference_end": 250, "reference_sequence": "MMKSPIGRYYPHETIIHKIDPRLKLIINIIYIVLTFIVNYFVSLAVLLVPLMIAFVIGSRKIYPLFRLMIMPLIIGFFILFVNIYTMKLKPDSDYTRIIPWGGHSEYALTLEALARTGALIIRIYIMIMVTSLLLITTKPILLTKALEDLMFPFKLLFIPTHIIAMIISIALRFIPTLFEEAQRIMKAQASRGVDFKNGKIKEKVKSFTTLIIPLFVTSFAKAEDLSNAMETRGYDPYAKRTRYRKLRIGWLDILFFILIVGLILFVAFSQSAKYDFLPFWYKFTYFKY" }, "paralog": false, "identity": 0.9885931558935361, "score": 496.0, "evalue": 3.14e-181, "description": "Internal stop codon at 893097, 893187, 893637. Nonsense mutation around 893097, 893187, 893637.", "psc": { "uniref90_id": "UniRef90_C4XFY5", "product": "Energy-coupling factor transporter transmembrane protein EcfT", "uniref50_id": "UniRef50_C4XFY5", "kegg_orthology_id": "K16785", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFY5", "UniRef:UniRef50_C4XFY5", "KEGG:K16785" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFY5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFY5" ], "product": "Energy-coupling factor transporter transmembrane protein EcfT" } }, "id": "OAIKOHHJFB_1601", "locus": "GCA000209735_01597" }, { "type": "cds", "sequence": "contig_1", "start": 893635, "stop": 893934, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATCTCCTATTGGTCGTTATTATCCACATGAAACAATAATTCATAAAATTGATCCAAGGTTAAAATTGATAATCAATATTATTTATATTGTTTTAACTTTTATTGTTAATTACTTTGTCTCTTTAGCTGTTTTATTAGTACCTCTAATGATTGCATTTGTTATTGGTTCAAGAAAAATATATCCTTTATTTAGATTAATGATTATGCCTCTAATTATTGGTTTCTTTATTCTCTTTGTTAATATCTATACTATGAAGTTAAAACCTGACTCGGACTATACAAGAATAATACCCTGA", "aa": "MKSPIGRYYPHETIIHKIDPRLKLIINIIYIVLTFIVNYFVSLAVLLVPLMIAFVIGSRKIYPLFRLMIMPLIIGFFILFVNIYTMKLKPDSDYTRIIP", "aa_hexdigest": "7a9ac08d0e5979782e99b999bd27eeb6", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11645.265499999985, "isoelectric_point": 9.9275671005249 }, "id": "OAIKOHHJFB_1602", "locus": "GCA000209735_01598" }, { "type": "cds", "sequence": "contig_1", "start": 893934, "stop": 894497, "strand": "-", "frame": 3, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003084074B" ], "nt": "TTGTTTGAATCTACTATTAAAGATGATATTGCTTTTGGACCAAGAAGCTTTGGTGTGCATAAAACTGAAGCTTATGAAAAAGCTAAAAAATATCTTGAGTTAGTAGGTTTGGATGAAAGTTACTTGAATAAATCACCTTTTGGTTTAAGTGGTGGTCAAAAAAGGCGTGTTGCTTTAGCTGGAATTTTAGCTATGGAACCCGACATTTTAATTGCTGATGAACCAACAGCTGGATTAGATCCAGTTGGAGTTAGAGAAGTTTTAGAAATTTTTAATAAACTTCATCAACAAGGTAAAACGGTTATTATTATTACACATGATTTGGATAATGTTTTAGAAGTAGCAAAAAGAGTATTAATTTTTAAAGATGGAAAAGTTGTTAAAGATGGTGATACTTATGAAGTATTAAGGAATACAAAATTCTTAAAAGAAAACAATATGGAACCTCCAAAATTATTAGAATTCGTTTCTAAATTAGAGGAAAGAGGCATTAGTGTGCCTAAAGTTACATCTATTGATGAACTAGCTGCATTCTTAAATAAAAGAATGAAAGGAGGTGTATAA", "aa": "MFESTIKDDIAFGPRSFGVHKTEAYEKAKKYLELVGLDESYLNKSPFGLSGGQKRRVALAGILAMEPDILIADEPTAGLDPVGVREVLEIFNKLHQQGKTVIIITHDLDNVLEVAKRVLIFKDGKVVKDGDTYEVLRNTKFLKENNMEPPKLLEFVSKLEERGISVPKVTSIDELAAFLNKRMKGGV", "aa_hexdigest": "4c0a8bb572a8e863dcff576ff069b0c5", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003084074B", "query_cov": 0.983957219251337, "subject_cov": 0.8598130841121495, "identity": 0.62, "score": 241.0, "evalue": 3.87e-78, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI003084074B" }, "pscc": { "uniref50_id": "UniRef50_UPI003084074B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003084074B" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_1603", "locus": "GCA000209735_01599" }, { "type": "cds", "sequence": "contig_1", "start": 894666, "stop": 894845, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGCAAATAAAAGTTAAAGATTTAGAACATATTTTTAGTCGTAGAACTCCTATGGAATTTAAAGCATTAAGTGGTATTAATGTAACAATCAATCAAGGCGAATATATAGGAATAATTGGACAAACTGGCTCAGGTAAAACTACATTCATAGAGCATTTAAATGCTCTTATTTTGCCATGA", "aa": "MQIKVKDLEHIFSRRTPMEFKALSGINVTINQGEYIGIIGQTGSGKTTFIEHLNALILP", "aa_hexdigest": "9a071ab99d2d6dc20b15bca2aa03d263", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 34, "aa_cov": 0.576271186440678, "hmm_cov": 1.0, "evalue": 1.0286937471976335e-08, "score": 31.96434783935547, "start": 23, "stop": 56 } ], "seq_stats": { "molecular_weight": 6570.593699999998, "isoelectric_point": 8.181505393981933 }, "id": "OAIKOHHJFB_1604", "locus": "GCA000209735_01600" }, { "type": "cds", "sequence": "contig_1", "start": 894836, "stop": 895633, "strand": "-", "frame": 1, "gene": null, "product": "energy-coupling factor transporter ATPase", "db_xrefs": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0042626", "RefSeq:WP_013354838.1", "SO:0001217", "UniParc:UPI0001E32F33", "UniRef:UniRef100_C4XFY7", "UniRef:UniRef50_Q98QH5", "UniRef:UniRef90_C4XFY7" ], "nt": "ATGATTAAAGTACAGGACCTAGTTTTTTCTTATCCAGAATCAAAAACAAAAGCTCTCAATGGGCTTAATTTTACCATTCCAGATGGTAAATATGTTGCTATATTAGGTCACAATGGATCAGGTAAAAGTACATTCAGTAAATTGCTTGTTGCTTTGTATAAACCTTCTTCAGGTTCTATTGAAATAAATGGAACAGTTATTTCTAAAAAAACAATCAGAGAAATAAGAAAGAAAATTGGCATTATTTTCCAAAACCCTGATAACCAATTTGTTGGCGCTAGTGTTGAAGATGATATTGCCTTTGGTTTAGAAAATCAATGCATTCCACAAAAAGAAATGAAACCTATTATTGATGATTTAGCTGAAAAAGTAGGAATGAAGGACTATTTGACAAGAGAACCTCAATTCCTTTCAGGAGGTCAAAAGCAACGCGTTGCCATAGCTTCTGTTTTAGCTTTAAATCCTGAAATCATAATTTTTGATGAAGTAACTTCAATGCTTGATCCAAGAGGTAAAAGTCAAGTTTTAAAAATTATTAGAGAAATCCAAAAAACTCGTCAAAAAACTTTAATTTCAATTACTCATGATATGGATGAAGCTATTCAAGCTGATTATTGCTTAGTTTTTTCTAAAGGTCAAGTTGTTGCAAGTGGCTCACCTAAAGAAATTTTAAAAAATCATGAAATCATTGAATTAGCTAAAATTGATTCACCTTTTATTTATAAGCTCAGTGAAAAAATTGAAAATATTGAATCAACATATAATGAAGAGGAGTTATTAAATCAACTATGCAAATAA", "aa": "MIKVQDLVFSYPESKTKALNGLNFTIPDGKYVAILGHNGSGKSTFSKLLVALYKPSSGSIEINGTVISKKTIREIRKKIGIIFQNPDNQFVGASVEDDIAFGLENQCIPQKEMKPIIDDLAEKVGMKDYLTREPQFLSGGQKQRVAIASVLALNPEIIIFDEVTSMLDPRGKSQVLKIIREIQKTRQKTLISITHDMDEAIQADYCLVFSKGQVVASGSPKEILKNHEIIELAKIDSPFIYKLSEKIENIESTYNEEELLNQLCK", "aa_hexdigest": "7e7533a266060b3d782fa1567b3db590", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E32F33", "ncbi_nrp_id": "WP_013354838.1", "uniref100_id": "UniRef100_C4XFY7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F33", "RefSeq:WP_013354838.1", "UniRef:UniRef100_C4XFY7" ] }, "ips": { "uniref100_id": "UniRef100_C4XFY7", "uniref90_id": "UniRef90_C4XFY7" }, "psc": { "uniref90_id": "UniRef90_C4XFY7", "product": "energy-coupling factor transporter ATPase", "uniref50_id": "UniRef50_Q98QH5", "go_ids": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QH5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QH5" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA1" }, "genes": [], "id": "OAIKOHHJFB_1605", "locus": "GCA000209735_01601" }, { "type": "cds", "sequence": "contig_1", "start": 895981, "stop": 896145, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTTCACGTGATGGAGACAATGCACCTATGGCTATTATTGAATTAGTTAAAGGCGACAAAGTTAAAACAAACAAAGCAGAAAAAACATCAAAAGACAAAGACACAAAAACTACAGCATCAAAAGCAACAAAAAGCAAAAAAGATGCTAAAGTAACTAAATAA", "aa": "MVSRDGDNAPMAIIELVKGDKVKTNKAEKTSKDKDTKTTASKATKSKKDAKVTK", "aa_hexdigest": "32932a419cbea1c214d45bd3906cd3f8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5837.681300000006, "isoelectric_point": 9.874316215515137 }, "id": "OAIKOHHJFB_1606", "locus": "GCA000209735_01602" }, { "type": "cds", "sequence": "contig_1", "start": 896454, "stop": 897467, "strand": "-", "frame": 3, "gene": null, "product": "DNA-directed RNA polymerase subunit alpha", "db_xrefs": [ "EC:2.7.7.6", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0046983", "RefSeq:WP_013354840.1", "SO:0001217", "UniParc:UPI0001E32F35", "UniRef:UniRef100_C4XFY9", "UniRef:UniRef50_D5E4Y4", "UniRef:UniRef90_C4XFY9" ], "nt": "ATGGAAAAAATGACAAAATTAGAGTACCAAAAGGTTACTAAATTAAATAATTCAACTTCAAATGTAACTGCATTTACTCTTCAACCACTTGAACGTGGTATGGGTCAAACATTAGGTGTTGCTCTTCGTCGTATTTTACTTTCAAACATTACTTCATTAGCATTATTTGCAATCAAAATTGAAGATGTTAATCATGAATTCCAAGTAGTTGATGGTTGCGTAGAAGACGTTGCCACAATTATTATGAATTTAAGAAAAGTAAAATTCCAATACAACCCAGAATTTGTTAAAGATGACGAAATTATTAAAGTAACATTAAAAGCTGATCAACCAGGAGAAGTTACTTCAAGATTATTAGAAGTAAACAACTCAAGTATTGAAATTTTAAACAAATCATTAGAAATTGCTCACTTATCAGCAAATGGTAAACACAAATTAAATATTGAATTATATTTAAGACCAGGTCGTGGCTTTATTTCTAATGAAGAAAATAAGAAATTACTTAATAACTCTTCAATTACAACTAGAATGGAATCAAAAATCAAAAACGGAATTTTTATTGCTACAGATAGTAACTTTAGTCCAATTGAAAAAGTTAACTACACAGTTGAAGAATTAAACTCATCATCAAACAAAATTGAAGAAAGATTAACCTTCTCATTCCAAACAGATGGAACAGTTGATCCTAAGAGTGCATTACAACAAGCTTGTGAAATCTTAGTTGGTCACTTCAAATTGATTGGTAATGTTGATGAAATGAAAGTAAATGTATTTGCAGAAGAAAGTGAACAAACTCAAGAAACAAACGACAATGATGTTGATATCAATTCATTAAATCTTTCGGTACGTTCATTGAATGCACTTAAAAGAATTGGTAAAACAAAGATTTCTCAAGTAGCTGAAATGTCACTTGAAGAATTAGAACAAGTTAAAAACCTTGGTCGTAAATCACTTGATGAAATTCAACAACGTTTAAAAGAATATGGATATGAACTAAGTAAAGGAGAAGAATAA", "aa": "MEKMTKLEYQKVTKLNNSTSNVTAFTLQPLERGMGQTLGVALRRILLSNITSLALFAIKIEDVNHEFQVVDGCVEDVATIIMNLRKVKFQYNPEFVKDDEIIKVTLKADQPGEVTSRLLEVNNSSIEILNKSLEIAHLSANGKHKLNIELYLRPGRGFISNEENKKLLNNSSITTRMESKIKNGIFIATDSNFSPIEKVNYTVEELNSSSNKIEERLTFSFQTDGTVDPKSALQQACEILVGHFKLIGNVDEMKVNVFAEESEQTQETNDNDVDINSLNLSVRSLNALKRIGKTKISQVAEMSLEELEQVKNLGRKSLDEIQQRLKEYGYELSKGEE", "aa_hexdigest": "e4a20ddfac56c0d6702415765ca2ce3c", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32F35", "ncbi_nrp_id": "WP_013354840.1", "uniref100_id": "UniRef100_C4XFY9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F35", "RefSeq:WP_013354840.1", "UniRef:UniRef100_C4XFY9" ] }, "ips": { "uniref100_id": "UniRef100_C4XFY9", "uniref90_id": "UniRef90_C4XFY9" }, "psc": { "uniref90_id": "UniRef90_C4XFY9", "product": "DNA-directed RNA polymerase subunit alpha", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_D5E4Y4", "go_ids": [ "GO:0003677", "GO:0003899", "GO:0006351", "GO:0046983" ] }, "pscc": { "uniref50_id": "UniRef50_D5E4Y4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E4Y4" ], "product": "DNA-directed RNA polymerase subunit alpha" }, "genes": [], "id": "OAIKOHHJFB_1607", "locus": "GCA000209735_01603" }, { "type": "cds", "sequence": "contig_1", "start": 897759, "stop": 897872, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAGAAAAACCAAAAAGAAAAATATAACAAATGGTGTAGCGCACATTCACTCAACAAACCAAAATACTATTGTTACCTTTGCTGACGAAAAAGGAAACGTAATTGCATGA", "aa": "MARKTKKKNITNGVAHIHSTNQNTIVTFADEKGNVIA", "aa_hexdigest": "edc136720f859cda4a2c66c2182ccb8f", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4051.5889000000006, "isoelectric_point": 10.171128654479979 }, "id": "OAIKOHHJFB_1608", "locus": "GCA000209735_01604" }, { "type": "cds", "sequence": "contig_1", "start": 897895, "stop": 898266, "strand": "-", "frame": 2, "gene": "rpsM", "product": "30S ribosomal protein S13", "db_xrefs": [ "GO:0000049", "GO:0003723", "GO:0003735", "GO:0005829", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0019843", "KEGG:K02952", "RefSeq:WP_013354842.1", "SO:0001217", "UniParc:UPI0001A8C14C", "UniRef:UniRef100_C4XFZ1", "UniRef:UniRef50_P38017", "UniRef:UniRef90_A5IYW1" ], "nt": "ATGGCTAGAATTTTAAATATTGAAATTCCTAACAATAAACGTGTTGTTATCTCATTAACATACATTTATGGTATAGGAAGAACTAGAGCACAAGAAATTTGTGCTAAAGCAAAAATTGATGAAAATCTTAGAGTTAAAGATTTATCAGAAGAACAATTATCAGCAATTCGTGAAGTAGCTAAAGAATACACAACTGAAGGTGATCTTCGTCGTGAAACCAGTTTAAACATCAAACGTTTAATGGAAATCAAATGCTACCGTGGTATTCGTCACAGAAAAGGTTTACCTGTTAGAGGTCAATGTACACAAAAGAATGCTCGTACACGTAAAGGTCCTCGTAAAACAGTTGCAGGTAAAAAATCAACTAAATAA", "aa": "MARILNIEIPNNKRVVISLTYIYGIGRTRAQEICAKAKIDENLRVKDLSEEQLSAIREVAKEYTTEGDLRRETSLNIKRLMEIKCYRGIRHRKGLPVRGQCTQKNARTRKGPRKTVAGKKSTK", "aa_hexdigest": "b9ae51f6d5f1f476d803c12f5915d571", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001A8C14C", "ncbi_nrp_id": "WP_013354842.1", "uniref100_id": "UniRef100_C4XFZ1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C14C", "RefSeq:WP_013354842.1", "UniRef:UniRef100_C4XFZ1" ] }, "ips": { "uniref100_id": "UniRef100_C4XFZ1", "uniref90_id": "UniRef90_A5IYW1" }, "psc": { "uniref90_id": "UniRef90_A5IYW1", "gene": "rpsM", "product": "30S ribosomal protein S13", "uniref50_id": "UniRef50_P38017", "kegg_orthology_id": "K02952", "go_ids": [ "GO:0000049", "GO:0003723", "GO:0003735", "GO:0005829", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_P38017", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P38017" ], "product": "Small ribosomal subunit protein uS13" }, "genes": [ "rpsM" ], "id": "OAIKOHHJFB_1609", "locus": "GCA000209735_01605" }, { "type": "cds", "sequence": "contig_1", "start": 898277, "stop": 898390, "strand": "-", "frame": 1, "gene": "rpmJ", "product": "50S ribosomal protein L36", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02919", "RefSeq:WP_013354843.1", "SO:0001217", "UniParc:UPI0001E32F37", "UniRef:UniRef100_A0AB32XCA5", "UniRef:UniRef50_P20278", "UniRef:UniRef90_A0A944MPI7" ], "nt": "ATGAAAGTTAGAGCTAGTGTAAAAAGAATGTGTAAAGATTGTCGTGTTATCAAACGTAAAGGAATTATTCGGGTAATTTGCTCACAACCAAAACACAAACAAAGACAAGGATAA", "aa": "MKVRASVKRMCKDCRVIKRKGIIRVICSQPKHKQRQG", "aa_hexdigest": "137a65f66db9c2840d9e402850c0e6b2", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001E32F37", "ncbi_nrp_id": "WP_013354843.1", "uniref100_id": "UniRef100_A0AB32XCA5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F37", "RefSeq:WP_013354843.1", "UniRef:UniRef100_A0AB32XCA5" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCA5", "uniref90_id": "UniRef90_A0A944MPI7" }, "psc": { "uniref90_id": "UniRef90_A0A944MPI7", "gene": "rpmJ", "product": "50S ribosomal protein L36", "uniref50_id": "UniRef50_P20278", "kegg_orthology_id": "K02919", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P20278", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P20278" ], "product": "Large ribosomal subunit protein bL36" }, "genes": [ "rpmJ" ], "id": "OAIKOHHJFB_1610", "locus": "GCA000209735_01606" }, { "type": "cds", "sequence": "contig_1", "start": 898408, "stop": 898623, "strand": "-", "frame": 2, "gene": "infA", "product": "translation initiation factor IF-1", "db_xrefs": [ "GO:0003743", "GO:0006413", "RefSeq:WP_013354844.1", "SO:0001217", "UniParc:UPI0001A8C14D", "UniRef:UniRef100_C4XFZ2", "UniRef:UniRef50_Q4A5I6", "UniRef:UniRef90_C4XFZ2" ], "nt": "ATGGCAAAAGACGCAATCAAAATGAAAGCTGTTGTCAAAGAAGCTTATTCAACAGATGAATATGATGTTGAACTTGAAAACGGCATGCTCATTAAAGCTCACATTTCAGGAAAAATGCGTGTAAATCACATTCGTATTTTGCCTGGAGATACTGTCGATGTTGAAATTAGTCCTTACAATTTATCTTTAGGACGAATAACATATCGTCACAAATAG", "aa": "MAKDAIKMKAVVKEAYSTDEYDVELENGMLIKAHISGKMRVNHIRILPGDTVDVEISPYNLSLGRITYRHK", "aa_hexdigest": "af11d2dbf559feacad294a6fa64b008c", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001A8C14D", "ncbi_nrp_id": "WP_013354844.1", "uniref100_id": "UniRef100_C4XFZ2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C14D", "RefSeq:WP_013354844.1", "UniRef:UniRef100_C4XFZ2" ] }, "ips": { "uniref100_id": "UniRef100_C4XFZ2", "uniref90_id": "UniRef90_C4XFZ2" }, "psc": { "uniref90_id": "UniRef90_C4XFZ2", "gene": "infA", "product": "translation initiation factor IF-1", "uniref50_id": "UniRef50_Q4A5I6", "go_ids": [ "GO:0003743", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5I6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5I6" ], "product": "Translation initiation factor IF-1" }, "genes": [ "infA" ], "id": "OAIKOHHJFB_1611", "locus": "GCA000209735_01607" }, { "type": "cds", "sequence": "contig_1", "start": 898720, "stop": 899373, "strand": "-", "frame": 2, "gene": "map", "product": "type I methionyl aminopeptidase", "db_xrefs": [ "EC:3.4.11.18", "GO:0006508", "GO:0070006", "KEGG:K01265", "SO:0001217", "UniRef:UniRef50_L0RW44", "UniRef:UniRef90_C4XFZ3" ], "nt": "ATGATAACTATTAAAAATGAACAAGAAATTATTAATATTACTCAAAGCTGCAAAATCCTGGCAGAAGTTAAACAAATAGTTTATGACTTTGTAAGACCAGGGGTTTCATTAAAAGAAGTGGATTCAGTCGCTTTTAATGAAATAGTAAAAAGAGGTGCACAACCAGCTTTTAAAGGCTTATATGGTTTTCCAGCAACCTGTTGCATCTCAATAAATGAAGAATTGATCCATGGAATTCCAAGTGATTACATTATTCAAGATGGCGACATAGTTAAAGTCGACATGGGATGTACTTACAAAGGAATGAACAGCGATAGTGCCTTCACTAAAGGTGTTGGTAATATCAATGAAAGTGATAAAAAAATAATTATAGTGGCTCAAGAATCATTCTATGCAGGTTTTAATGCGATTAAACCTGGTGCAAGAGTGGGAGATGTTAGTCACGCTATTGGTCAAGTAATAAGACAAAATAATTTATATACACCAAGTCAATACTGTGGGCATGGAATTGGACGTGAAGTTCATGAAGACCCTTATGTATTTAATGATGGCCATGCTGGTAGTGGACCATTATTAAAAGATGGAATGGTAATTTGTATTGAGCCAATGATTTTACAACAAAATGCTAGAATCAAAGTTAAAAAAGATAAATGA", "aa": "MITIKNEQEIINITQSCKILAEVKQIVYDFVRPGVSLKEVDSVAFNEIVKRGAQPAFKGLYGFPATCCISINEELIHGIPSDYIIQDGDIVKVDMGCTYKGMNSDSAFTKGVGNINESDKKIIIVAQESFYAGFNAIKPGARVGDVSHAIGQVIRQNNLYTPSQYCGHGIGREVHEDPYVFNDGHAGSGPLLKDGMVICIEPMILQQNARIKVKKDK", "aa_hexdigest": "048d065444cdc761b752145271a35e41", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_C4XFZ3", "query_cov": 1.0, "subject_cov": 0.8645418326693227, "identity": 1.0, "score": 437.0, "evalue": 1.45e-154, "valid": true, "gene": "map", "product": "type I methionyl aminopeptidase", "ec_ids": [ "3.4.11.18" ], "uniref50_id": "UniRef50_L0RW44", "kegg_orthology_id": "K01265", "go_ids": [ "GO:0006508", "GO:0070006" ] }, "pscc": { "uniref50_id": "UniRef50_L0RW44", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RW44" ], "product": "Methionine aminopeptidase" }, "genes": [ "map" ], "id": "OAIKOHHJFB_1612", "locus": "GCA000209735_01608" }, { "type": "cds", "sequence": "contig_1", "start": 899401, "stop": 900063, "strand": "-", "frame": 2, "gene": null, "product": "Adenylate kinase", "db_xrefs": [ "RefSeq:WP_013354846.1", "SO:0001217", "UniParc:UPI0001E32F39", "UniRef:UniRef100_C4XFZ4", "UniRef:UniRef50_F9UK13", "UniRef:UniRef90_C4XFZ4" ], "nt": "ATGATAAGTAAAATTAAACAACATATAGTTTTTATGGGGCCTCCTGGTGTAGGAAAAGGTACAGTGGCTGCTATAATAGCTGAAAAATATGGACTAGTACATGTTTCAACTGGTAATATCTTTCGTGAAGAAATAGCTTCACAAAGTGAACTTGGTTTACAAGTTAAAAAGATAGTTGAAGCAGGACAATATGTACCAGACGACATTACAAATACTATTGTTAAAAATAAAATTCAAGCTTTAATCAAAGCTAAAAAGATTATTATGCTTGATGGTTATCCACGTACTATTGACCAAGTTAACTTTTTAGATAGTATTTCAAACTTTAAATATGTAGCTCTTGAATTAAGTGCTCCTGAAGATGTTATTTTAAAAAGATTAAATGGACGTAGACAATGTCCTAAATGTAAAGCAAGTTTCCATATTGATTTTATGCCTTCAACTAAAGGCAAATATTGTGATAAATGTGGAGCTGAATTAATCTTAAGAAAAGACGACTCAATTGAAGCTATTAAAGTACGTCAAAAAGTTTATCATGATCAAACAGCACCATTGCTTGATTATTACAAAAAATATAATTTATTATTAACATTTGATGCTACAAGTGATCCAGAAACTATAGCAAAAAATATTGTTGAATCTTTAGCGAAATTAAAAAACTAA", "aa": "MISKIKQHIVFMGPPGVGKGTVAAIIAEKYGLVHVSTGNIFREEIASQSELGLQVKKIVEAGQYVPDDITNTIVKNKIQALIKAKKIIMLDGYPRTIDQVNFLDSISNFKYVALELSAPEDVILKRLNGRRQCPKCKASFHIDFMPSTKGKYCDKCGAELILRKDDSIEAIKVRQKVYHDQTAPLLDYYKKYNLLLTFDATSDPETIAKNIVESLAKLKN", "aa_hexdigest": "d16dd8f9994701b2e5cea4a7b2b1b031", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001E32F39", "ncbi_nrp_id": "WP_013354846.1", "uniref100_id": "UniRef100_C4XFZ4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32F39", "RefSeq:WP_013354846.1", "UniRef:UniRef100_C4XFZ4" ] }, "ips": { "uniref100_id": "UniRef100_C4XFZ4", "uniref90_id": "UniRef90_C4XFZ4" }, "psc": { "uniref90_id": "UniRef90_C4XFZ4", "product": "Adenylate kinase", "uniref50_id": "UniRef50_F9UK13" }, "pscc": { "uniref50_id": "UniRef50_F9UK13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_F9UK13" ], "product": "Adenylate kinase" }, "genes": [], "id": "OAIKOHHJFB_1613", "locus": "GCA000209735_01609" }, { "type": "cds", "sequence": "contig_1", "start": 900060, "stop": 900284, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGATTTTAGTATCAGTTGCTCTTGAAACTATTCAACAATTCATGGCACGCCGTAAAAGTCAACATCTTGCAAAACAAAAACGTCTTTCAAAAGAAGCTGCAGATGTGCTTAACTTCCAAAACAAATATCAAGAAAATGATCCTTTTGCAACAAATGATCAAAAATCTGAAGACGTTTCAAAAGAATATGATAAATACAAAAATGGAGATGGCTTATTATGA", "aa": "MMILVSVALETIQQFMARRKSQHLAKQKRLSKEAADVLNFQNKYQENDPFATNDQKSEDVSKEYDKYKNGDGLL", "aa_hexdigest": "1c758825e9f37433c9856efbff4ba77e", "start_type": "ATG", "rbs_motif": "GGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8595.625100000001, "isoelectric_point": 7.965729713439941 }, "id": "OAIKOHHJFB_1614", "locus": "GCA000209735_01610" }, { "type": "cds", "sequence": "contig_1", "start": 900324, "stop": 900578, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATTCACTCAACCACTAGGCTTGTCATTATTGATTCTAATTACATTCTTCTTTAGTTACATAATTGGATTGCAACAATCAAGAATTGACAAGATTTCAGAAGACTTTGCAAAGAACTCAACATTTATTCCTGGTATTAGACCTGGAGAAGAAACTGAAGATTATTTAATAGGTGTTGTTTTAAGACTTTGCACCTTCAGTGGTTTCTATTTAGTATTCTTAGCTTCATTCCAATACATAATGATTTTATGA", "aa": "MQFTQPLGLSLLILITFFFSYIIGLQQSRIDKISEDFAKNSTFIPGIRPGEETEDYLIGVVLRLCTFSGFYLVFLASFQYIMIL", "aa_hexdigest": "0bb7717a2da83d7017885cd2d4ccfd93", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9640.237099999995, "isoelectric_point": 4.715101432800293 }, "id": "OAIKOHHJFB_1615", "locus": "GCA000209735_01611" }, { "type": "cds", "sequence": "contig_1", "start": 900591, "stop": 901451, "strand": "-", "frame": 3, "gene": "secY", "product": "Preprotein translocase subunit SecY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PVK7" ], "nt": "TTGCTTTATACATTATTGTTGTTATCAATCTATATTTTAATGACAACAATCAAAGCTCCTTTTGTTAAATTATCTGATGTAAATATTAATGATGACCCATTCTTAAATACATTAAACCTTGTTGGCGGTGGAGGTCTTAAAAACTTCTCACTTGCAGCACTTGGAATTAGTCCATTCATTAATGCCAGCTTGATAATGTCTCTTTTACAAACCAAACTTTTCCCTCCAATTCAAAAACTTAGTCAAAGTGGACCACAAGGTCGGAGAAAATTAAATATCATTACAAGAATTTTGACTTTGTTTATTGCATTCCCTCAAGCTATCTTGCTTACTAAATCACTTAGTTTAGGTGACAATCCATTTATTGGATTTACTCCAATTCAAAAACTCCCTCGAGAAGTTACAATCTACTTAATATTGCCTTTAATCTTAATTGGTGGATCATTGTTCTCATTATTCATTGCTGAACAAATTACAGATAAAGGTTTAGGAAATGGTACAAGCTTAATTATTTTTGTTGGTATTGCATTCCAATTACAAAGTCAATTTAAAGGAGCATTTAGCTTATTTGTTGGTAGTGAAAGTACAGCAGCAATTTTTGTTGGAGCATTAAAATTCTTAACTTACTTATTTGTTTACTTTGCATTGTTATTTATTATTGCTTTAGTTTACAATGCTGAAAGACATGTACCTATTCAACAAATTGGTGCTGGACGTAGTAGAAATATTAATGAAATGGGTAAATTGCCAATTAAATTAAATCCCGGTGGAATTATGCCAATAATCTTCGCCTCAATGCTAGTCAGCTTCCCAATTATGATATCTAGAATACTTCCAAAAGGTAATTCTGGCAAATTATGA", "aa": "MLYTLLLLSIYILMTTIKAPFVKLSDVNINDDPFLNTLNLVGGGGLKNFSLAALGISPFINASLIMSLLQTKLFPPIQKLSQSGPQGRRKLNIITRILTLFIAFPQAILLTKSLSLGDNPFIGFTPIQKLPREVTIYLILPLILIGGSLFSLFIAEQITDKGLGNGTSLIIFVGIAFQLQSQFKGAFSLFVGSESTAAIFVGALKFLTYLFVYFALLFIIALVYNAERHVPIQQIGAGRSRNINEMGKLPIKLNPGGIMPIIFASMLVSFPIMISRILPKGNSGKL", "aa_hexdigest": "cc596245aea8acce8a3215553ca8da1b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0024CD17B9", "query_cov": 0.9090909090909091, "subject_cov": 0.8843537414965986, "identity": 0.6, "score": 287.0, "evalue": 9.72e-94, "valid": false, "product": "preprotein translocase subunit SecY", "uniref50_id": "UniRef50_A0A3B0PVK7" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PVK7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PVK7" ], "product": "Preprotein translocase subunit SecY" }, "genes": [ "secY" ], "id": "OAIKOHHJFB_1616", "locus": "GCA000209735_01612" }, { "type": "rRNA", "sequence": "contig_1", "start": 901870, "stop": 904753, "strand": "-", "gene": "rrl", "product": "23S ribosomal RNA", "coverage": 0.9859829059829059, "score": 2776.1, "evalue": 2e-06, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF02541", "KEGG:K01980", "SO:0001001" ], "nt": "TTTTAAATAAGGAAGAGTTTGTGGTGGATGCCTTGGGTCTGAAAGTCGATGAAGGACGTGATTACCTGCGATAAGCCTCGTGGAGCCGGAAATATGCTATGAAACGGGGATTTCCGAATGGGGAAACCTAACTAGGGTAATGCCTAGTTGCCTTGTTCTGAATTCATAGGAACTTGAGTGAGACACCTTGTGAACTGAAACATCTTAGTAGCAAGAGGAAGAGAAAATAAACAATGATTTCTTTAGTAGCGGCGAGCGAACGAGAAAGAGCCCAAACCCAGCAATGGGGGTTGTAGGACGATCTACATGAAGTTACAAAACTTAATGATAGCAGAAGCTTTTGGGAAGAAGCGGCATAGAGGGTGATACCCCCGTATGTGAAATTGTTAAGTCTTCTGATCGTATCCTGAGTAGGGCGGGGCACGTGGAACCCTGTCTGAATCTGCCGGGACCACCCGGTAAGGCTAAATACTAATCAGACACCGATAGTGAACTAGTACCGTGAGGGAAAGGTGAAAAGAACCCCGAGAGGGGAGTGAAATAGATTCTGAAACCACTTACTTACAATTAGTCAGAGCCCGTTTATGGGTGATGGCGTACATCTTGCAGTATGGACCGGCGAGTTATGACAGCATGCGAGGTTAAGCAGAAAAAAGCGGAGCCGTAGAGAAATCGAGTCTTAATAGGGCGTCTTAGTATGTTGTCATATACCCGAAACCATGTGATCTATTCATGAGCAGGCTGAAACTTGGCTAACCCCAAGCGGAGGGCCGAACCGTAGTACGCTGAAAAGTGCCCGGATGACTTGTGAATAGTGGAGAAATTCCAATCGAACTTGGAGATAGCTGGTTCTCCTCGAAATAGCTTTAGGGCTAGCGTGTAGTGTTAAGTAGTGGTGGTAGAGCACTGAATATGGAATGGCCTCGCCTAGGGGTACTGACTATAATCAAACTCCGAATACCGCTATGTATTGCTATGCAGTCGGAACCGGGGTGCTAACGTCCCGGCTCGCGAGGGCAACAACCCAGATCGCCGGCTAAGGTCCCAAAATCGTGTTAAGTGAGAAAGGTTGTGAGGTTTCATAAACAACTAGGAAGTTGGCTCAGAAGCAGCCACCTTTTAAAGAGTGCGTAATAGCTCACTAGTCAAGAGACCTTGCGCCAACAATTTAACGGGACTAAAACACGATACCGAAGCCGCGGGTAATTATATTTACGTTAGAGGAGCGTTCCTAGGGCAATGAAGTCAGACCGTGAGGACTGGTGGAGCGCTAGGAAGTGAGAATGCCGGTATGAGTAACGATTCGTAGTGAGAATCTACGACGCCTATTGGGGAAGGTTTCCTGGGCAAGGTTCGTCCACCCAGGGTTAGTCAGGACCTAAGACGAGGCTGAAAAGCGTAGCCGATGGACAACAGGTTAATATTCCTGTACTTTCTATAATAGTGATGGAGTGACGGAGGAGGATAATCTTACCACTTACTGGATTGTGGGGTAAGCGTTCACTGGTGACTGTAGGCAAATCCGCAGTCTATAACCGGGAGGCGTGATGCATAGGGTCAAACCGAATTAGATGATTCCACACTTCCAAGAAAAGCTTCTAAACTTAATATTATGGAAACCTGTACCGAGAACGGACACACGTCCCCGAGATGAGTATTCTAAGGCGAGCGAGAAAACTAGTGTTAAGGAACTCTGCAAAATGACCCCGTAAGTTCGCAAGAAGGGGCGCCTAAGGTAACACTTAGGCCACAGTAAATTATGAGGGGCAACTGTTTATCAAAAACACAGCTCTCTGCTAAACCGCAAGGTGATGTATAGGGGGTGAAGCCTGCCCAGTGCCCGAAGGTTAAGTGGATGCGTTAGCATTTGCGAAGCGTTGAAATGAAGCCCGGGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGCTAAATACTGACCTGCACGAAAGGCGCAATGATCTCTCAACTGTCTCAACACTAGACTCGGTGAAATTATGGTCCCAGTGAAAACGCTGGGTACCCGCATCAAGACGAAAAGACCCCATGGAGCTTTACTACAGTTTCGTATTGGAACTTGGTCTAACATGTGTAGGATAGGTGGGAGACTTTGAAGCGAGGACGCTAGTTCTCGTGGAGTCATCCTTGAAATACCACCCTTGGTATATTGAGTTTCTAACATGCCATCATCATCAGGTGGGTGGACAGTGCGTGACGGGTAGTTTGACTGGGGCGGTCGCCTCCTAAAGAGTAACGGAGGCGTTCAAAGGTACACTCAGTACGGTCAGAAACCGTATGTAGAGCGCAAAGGTAGAAGTGTGCTTGACTGTGAGACTTACAAGTCGAGCAGGTGCGAAAGCAGGACTTAGTGATCCGGCTGTACATTGTGGAATGGCAGTCGCTCAACGGATAAAAGTTACCCTGGGGATAACAGGCTTATCTTGCCCAAGAGATCACATCGACGGCAAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGGAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAAAACGTCGTGAGACAGTTTGGTCCCTATCTGATGTGGGCGTTGGAATATTGATGAGAGCCGCTCTTAGTACGAGAGGACCGGAGTGGACGTACCACTGGTGTTCCAGTTGTTCCGCCAGGAGCATAGCTGGGTAGCTAAGTACGGAAAGGATAACCGCTGAAAGCATCTAAGTGGGAAGCCTCCTCAGAGATAAGTATTCCCTTGAGATTCCTTATAGACTATGAGGTTGATAGGTTGGAGGTGTAAGCGTAGTAATACGTTGAGCTGACCAATACTAATAAATCGATCGGTTTAAAAGT", "id": "OAIKOHHJFB_41", "locus": "GCA000209735_01613" }, { "type": "rRNA", "sequence": "contig_1", "start": 905067, "stop": 906579, "strand": "-", "gene": "rrs", "product": "16S ribosomal RNA", "coverage": 0.9869536855838226, "score": 1507.4, "evalue": 1.6e-46, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00177", "KEGG:K01977", "SO:0001000" ], "nt": "TTTTTCGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCTGTGTGCCTAATACATGCATGTCGAGCGAAGGTAGCAATACCTTAGCGGCGAATGGGTGAGTAACACGTGCTCAACGTACCCTTCAGTTTGGCATAGCGACTGGAAACAGTCGATAATTTCAAATACTCGTAGTTTTCGCATGAAGATTACGGAAAAGAAGCGTTTGCTTCGCTGGAGGAGCGGGGTGCGTAACATTAGCTAGTTGGTGAGGTAACGGCCCACCAAGGCGATGATGTTTAGCGGGGTTGAGAGACTGAACCGCCACACTGGGACTGAGATACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATATTCCACAATGGGCGAAAGCCTGATGGAGCGACACAGCGTGAAGGATGAAGGTCCTATGGATTGTAAACTTCTGTGGTAAGGGAAGAAAAGACAGAATAGGAAATGATTTTGTTTTGACGGTACCTTATTAGAAAGCAACGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGTTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCTGTAGGTTGTTTGTTAAGTCTGGCGTTAAATTTTGGGGCTCAACCCCAAAACGCGTTGGATACTGGCAGGCTAGAGTTGTGTAGAGGTTAGCGGAATTCCTTGTGAAGCGGTGAAATGCGTAGATATAAGGAAGAACACCAAGATGGCGAAGGCAGCTAACTGGACATATACTGACACTGAGAGACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGATCATTAGCTGATGGGGAACTCATCGGCGCAGCTAACGCATTAAATGATCCGCCTGAGTAGTACGTTCGCAAGAATAAAACTTAAAGGAATTGACGGGGATCCGCACAAGCGGTGGAGCATGTGGTTTAATTTGAAGATACGCGTAGAACCTTACCCACTCTTGACATCTTCTGCAAAGCTATGGAGACATAGTGGAGGTTAACAGAATGACAGATGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTTGGTTAAGTCCTGCAACGAGCGCAACCCTTATCCTTAGTTACTACCATTTAGTTGAGGACTCTAAGGAGACTGCCCGAGTAATCGGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCTCTTACGAGTGGGGCAACACACGTGCTACAATGGCCGGTACAAAGAGAAGCGAAGTGGTGACATGGAGCAAACCTCAAAAAACCGGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGTCGGAATCGCTAGTAATCGTAGATCAGCTACGCTACGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACCATGGGAGCTGGTAATGCCCGAAGTCGGTTTATAAACAAACTGCCTAAGGCAGGACTGGTGACTGGGGTTAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATCACCTCCTTT", "id": "OAIKOHHJFB_38", "locus": "GCA000209735_01614" }, { "type": "cds", "sequence": "contig_1", "start": 906903, "stop": 907025, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00828.25" ], "nt": "ATGCCTGTTAAATTATTGGGTAATGGTCAAGTAACTAAAAAATTCAATGTTAATCTTCATGCAGCTTCAAAAAGTGCTATTGATGCAATCGAAGCTAAAGGTGGAAAAGTTACTTTATTATAG", "aa": "MPVKLLGNGQVTKKFNVNLHAASKSAIDAIEAKGGKVTLL", "aa_hexdigest": "c78cf49d5fc03939e8b08fbbd696d387", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00828.25", "name": "Ribosomal_L27A", "length": 37, "aa_cov": 0.925, "hmm_cov": 1.0, "evalue": 8.866086698178506e-15, "score": 51.03211212158203, "start": 2, "stop": 38 } ], "seq_stats": { "molecular_weight": 4162.896099999999, "isoelectric_point": 10.000287437438963 }, "id": "OAIKOHHJFB_1617", "locus": "GCA000209735_01615" }, { "type": "cds", "sequence": "contig_1", "start": 907173, "stop": 907343, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTCAACATTACAATCATTAAGACCTACCGCTGGCTCACGTGTTGAAAAACACCGTGTTGGTCGCGGACATGCTGCTGGAAAAGGTAAACAAGCTGGTAAAGGTCAAAGTGGACAAAACAAACGTAAGGGTCACAGACTAGGATTTGAAGGTGGTCAAACACCTTGA", "aa": "MNSTLQSLRPTAGSRVEKHRVGRGHAAGKGKQAGKGQSGQNKRKGHRLGFEGGQTP", "aa_hexdigest": "dfca84a96eb9aa8097633e2899427aab", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5893.543500000002, "isoelectric_point": 11.999967765808105 }, "id": "OAIKOHHJFB_1618", "locus": "GCA000209735_01616" }, { "type": "cds", "sequence": "contig_1", "start": 907398, "stop": 907616, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03719.21" ], "nt": "ATGCTTAAACCTGATACAAAAGGTAAAGGTATTGTTGCTAGTGGTACAGTTAGAGCAGTTGTTGAACTTGCAGGTTACACCGATATTTATACCAAAACTTATGGTTCACGTTCAAAAGCAAACATTGTTAGAGCAACTTTAAAAGCTCTTCAAGCTTTAAGAACACCAGAACAAATTGCTGACATTCGTGGTAAAGATGTTAAAGATCTTTTAGCCTAA", "aa": "MLKPDTKGKGIVASGTVRAVVELAGYTDIYTKTYGSRSKANIVRATLKALQALRTPEQIADIRGKDVKDLLA", "aa_hexdigest": "747a25fc2302c2a9876327d5c14d9336", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03719.21", "name": "Ribosomal_S5_C", "length": 63, "aa_cov": 0.875, "hmm_cov": 1.0, "evalue": 1.643763967136042e-25, "score": 84.45747375488281, "start": 1, "stop": 63 } ], "seq_stats": { "molecular_weight": 7746.9394999999995, "isoelectric_point": 9.91454448699951 }, "id": "OAIKOHHJFB_1619", "locus": "GCA000209735_01617" }, { "type": "cds", "sequence": "contig_1", "start": 907983, "stop": 908108, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGAAGCAGAAGTAAAGAAAGTTGTTAATAAAGAACAACCTAAAGAAGCCCAAGCTCAAGGAACTAAAGAAGTTGTAGCTCCTAAAGCTAAAGCTGAAAACAAAGATAAAAAACAAATTTGA", "aa": "MAEAEVKKVVNKEQPKEAQAQGTKEVVAPKAKAENKDKKQI", "aa_hexdigest": "4287dce5a67b631dc5ca2eaa847fb5dc", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4520.171200000001, "isoelectric_point": 9.399055290222169 }, "id": "OAIKOHHJFB_1620", "locus": "GCA000209735_01618" }, { "type": "cds", "sequence": "contig_1", "start": 908111, "stop": 908476, "strand": "-", "frame": 1, "gene": "rplR", "product": "50S ribosomal protein L18", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354765.1", "SO:0001217", "UniParc:UPI0001E32EBC", "UniRef:UniRef100_C4XFZ9", "UniRef:UniRef50_Q4A5D7", "UniRef:UniRef90_A0A3P8LIJ1" ], "nt": "ATGGCTACATTATCTCGTAATCAAGCACGTCAAGTTAAACACTTACGTGAACGTCAAAGTATTTCAGGTACTGCTACTAAACCACGTCTTTGCGTATTCAAATCTCATACAAACTTTTATGCACAACTAATTGACGATACAACAGGACACACTTTAGCAAGTGTTTCAACACTTGATCCTAAAGCTAAAGTTAAATACCACGGAAACATTGCTTCAGCAACTAAAGCTGGTGAAGCAATGGGTGCTAAAATTGCAGCCCTTAAATTAAAAGAAATAGTATTCGATCGTTCAGGTTACATTTATCATGGACGTGTTAAAGCCTTCGCAGAAGGTGTTAGAAGTATAGCTAAAGGAGTTAAATTCTAA", "aa": "MATLSRNQARQVKHLRERQSISGTATKPRLCVFKSHTNFYAQLIDDTTGHTLASVSTLDPKAKVKYHGNIASATKAGEAMGAKIAALKLKEIVFDRSGYIYHGRVKAFAEGVRSIAKGVKF", "aa_hexdigest": "58b59e311212f3d90497aedf3886588a", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001E32EBC", "ncbi_nrp_id": "WP_013354765.1", "uniref100_id": "UniRef100_C4XFZ9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EBC", "RefSeq:WP_013354765.1", "UniRef:UniRef100_C4XFZ9" ] }, "ips": { "uniref100_id": "UniRef100_C4XFZ9", "uniref90_id": "UniRef90_A0A3P8LIJ1" }, "psc": { "uniref90_id": "UniRef90_A0A3P8LIJ1", "gene": "rplR", "product": "50S ribosomal protein L18", "uniref50_id": "UniRef50_Q4A5D7", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5D7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5D7" ], "product": "Large ribosomal subunit protein uL18" }, "genes": [ "rplR" ], "id": "OAIKOHHJFB_1621", "locus": "GCA000209735_01619" }, { "type": "cds", "sequence": "contig_1", "start": 908503, "stop": 909042, "strand": "-", "frame": 2, "gene": "rplF", "product": "50S ribosomal protein L6", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "RefSeq:WP_013354766.1", "SO:0001217", "UniParc:UPI0001E32EBD", "UniRef:UniRef100_C4XG00", "UniRef:UniRef50_Q4A5D6", "UniRef:UniRef90_C4XG00" ], "nt": "ATGTCTCGTGTTGGAAATAGAGTATTAAATATCCCTGCAGGTGTTGAAGTTAATTTAAATGGTACAACCTTAAATGTTAAAGGTTCATTAGGCCAATTAGAAAAAACATTCAGCCCACTTATTGCAATTAAAATTGAAAATAATCAAATTACAACCCTCCGTGCTAATGAAGAAAAACACACAAAACAATTACACGGAACAACAAATGCTTTAATTCAAGGCATGCTTGAAGGTGTTTCAAAAGGTTTCAAAAAAGAATTAGCAATCAAAGGTGTTGGTTACAAAGCTGCACTTAATGGTCAAACTTTAGAATTAACTGTTGGTTACAGCCACATAGTTAGTCTAAGTGTACCAGAAGGTGTTAAAGTTGAAGTTCCAAAACCTCTTGAAATCATTATTAGTGGTATTGACAAAGAAAAAGTTGGTCAATTTGCTGCTGTTGTGCATGATGTTCGTCGTCCTAACTCATACTCAGGTAAAGGGATTTCATACAAAGGCGAATACATTCGTATTAAAGAAGGAAAAACTGCTTCTAAATAG", "aa": "MSRVGNRVLNIPAGVEVNLNGTTLNVKGSLGQLEKTFSPLIAIKIENNQITTLRANEEKHTKQLHGTTNALIQGMLEGVSKGFKKELAIKGVGYKAALNGQTLELTVGYSHIVSLSVPEGVKVEVPKPLEIIISGIDKEKVGQFAAVVHDVRRPNSYSGKGISYKGEYIRIKEGKTASK", "aa_hexdigest": "1d3b64968fc4c18bc8af2cf04abb7e59", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI0001E32EBD", "ncbi_nrp_id": "WP_013354766.1", "uniref100_id": "UniRef100_C4XG00", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EBD", "RefSeq:WP_013354766.1", "UniRef:UniRef100_C4XG00" ] }, "ips": { "uniref100_id": "UniRef100_C4XG00", "uniref90_id": "UniRef90_C4XG00" }, "psc": { "uniref90_id": "UniRef90_C4XG00", "gene": "rplF", "product": "50S ribosomal protein L6", "uniref50_id": "UniRef50_Q4A5D6", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5D6" ], "product": "Large ribosomal subunit protein uL6" }, "genes": [ "rplF" ], "id": "OAIKOHHJFB_1622", "locus": "GCA000209735_01620" }, { "type": "cds", "sequence": "contig_1", "start": 909057, "stop": 909449, "strand": "-", "frame": 3, "gene": "rpsH", "product": "30S ribosomal protein S8", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "SO:0001217", "UniRef:UniRef50_Q6MSN9", "UniRef:UniRef90_C4XG01" ], "nt": "ATGTTTATCACAGATCCAATTTCAGATATGATTGTTAGAATCAAAAACGCTAATCAACGTAAATTCAAAACTGTAGCTATTCCTTTTTCAAATAAAAAAGCTAAAATTCTAGAAATTCTTCAAAATGAAGGTTACATTACAAACTTTGTTACAAAAGGCCAAGGTAAAGACAAAGTACTTGAAGTTTCATTAAAATACAAAAACAACCAAAGTGCAATTGTTGACTTTAAACGTGTTTCAAAACCAGGTCTTAAAGTTTATACTTCAGCTGACAAATTACCTACCATTTTATCAGGTTATGGTACAGCAATCATTTCAACTTCAAAAGGAATGATGACTGAAAAACAAGCTCGTAAGGAAAATGTGGGTGGTGAAATCATTGCCTACATTTGA", "aa": "MFITDPISDMIVRIKNANQRKFKTVAIPFSNKKAKILEILQNEGYITNFVTKGQGKDKVLEVSLKYKNNQSAIVDFKRVSKPGLKVYTSADKLPTILSGYGTAIISTSKGMMTEKQARKENVGGEIIAYI", "aa_hexdigest": "a5856a56c4197a0d5098d6f8d5af4378", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_C4XG01", "query_cov": 1.0, "subject_cov": 0.9701492537313433, "identity": 1.0, "score": 250.0, "evalue": 4.25e-84, "valid": true, "gene": "rpsH", "product": "30S ribosomal protein S8", "uniref50_id": "UniRef50_Q6MSN9", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q6MSN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MSN9" ], "product": "Small ribosomal subunit protein uS8" }, "genes": [ "rpsH" ], "id": "OAIKOHHJFB_1623", "locus": "GCA000209735_01621" }, { "type": "cds", "sequence": "contig_1", "start": 909455, "stop": 909637, "strand": "-", "frame": 1, "gene": "rpsZ", "product": "type Z 30S ribosomal protein S14", "db_xrefs": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0008270", "GO:0015935", "GO:0019843", "KEGG:K02954", "SO:0001217", "UniRef:UniRef50_A5IYX3", "UniRef:UniRef90_A5IYX3" ], "nt": "ATGGCTAAAGTTTCATTAAAAGCAAAACAAAAGAAACATCCTAAATTCTCAACACGTGCTTACACACGTTGTGAATTATGTGGACGTCCACACGCAGTTTTAAGAAAATACAAGATTTGCCGTATTTGTTTCCGTAATTTAGCTCATGAAGGAAAAATTCCTGGCATGAAGAAAGCGAGTTGA", "aa": "MAKVSLKAKQKKHPKFSTRAYTRCELCGRPHAVLRKYKICRICFRNLAHEGKIPGMKKAS", "aa_hexdigest": "d8ef3eb6eb98730ca40562b821f7b0c7", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A5IYX3", "query_cov": 1.0, "subject_cov": 0.8823529411764706, "identity": 1.0, "score": 127.0, "evalue": 2.79e-37, "valid": true, "gene": "rpsZ", "product": "type Z 30S ribosomal protein S14", "uniref50_id": "UniRef50_A5IYX3", "kegg_orthology_id": "K02954", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0008270", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_A5IYX3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IYX3" ], "product": "Small ribosomal subunit protein uS14" }, "genes": [ "rpsZ" ], "id": "OAIKOHHJFB_1624", "locus": "GCA000209735_01622" }, { "type": "cds", "sequence": "contig_1", "start": 909639, "stop": 909866, "strand": "-", "frame": 3, "gene": null, "product": "50S ribosomal protein L5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0NY94" ], "nt": "ATGCCACGTATTCGTGATTTCCGTGGTGCCAACCCTAAAGCATTCGATGGTAGAGGAAACTACTCATTAGGTGTTAAAGAAGAAATTATTTTCCCAGAAATCGAATTTGACAAAATTCGTCACATCAAGGGACTTGATGTTCAATTAATCACAAGCACAAATAAAGATGAAGAAGCTAGAAGTTTATTAGAATTAATCGGCATTCCATTTATTAAAGGAGATAGATAA", "aa": "MPRIRDFRGANPKAFDGRGNYSLGVKEEIIFPEIEFDKIRHIKGLDVQLITSTNKDEEARSLLELIGIPFIKGDR", "aa_hexdigest": "e529e4da82ed9014b0ac75de865e36ee", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0NY94", "query_cov": 0.9866666666666667, "subject_cov": 0.9866666666666667, "identity": 0.595, "score": 104.0, "evalue": 6.4e-28, "valid": false, "product": "50S ribosomal protein L5", "uniref50_id": "UniRef50_A0A3B0NY94", "kegg_orthology_id": "K02931" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0NY94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0NY94" ], "product": "50S ribosomal protein L5" }, "genes": [], "id": "OAIKOHHJFB_1625", "locus": "GCA000209735_01623" }, { "type": "cds", "sequence": "contig_1", "start": 909957, "stop": 910184, "strand": "-", "frame": 3, "gene": null, "product": "50S ribosomal protein L5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X3HCF5" ], "nt": "ATGAATTTAAAAGCTTATTACACAAAAACAGTTGTACCTGCATTAATGAAAAAATATAACTATTCATCAGTTATGCAAGTACCACGTTTAGAAAAAATTGTTTTGAATATGACAGCAGGTAAAGAAGTAACAAACTCTAAAGCTATCGAAGAAGTTCTTAATGAACTTACTCAAATTTCAGGTCAAAAACCATTCCAAACCAGAGCTAAAAAATCAAATGCTTCATGA", "aa": "MNLKAYYTKTVVPALMKKYNYSSVMQVPRLEKIVLNMTAGKEVTNSKAIEEVLNELTQISGQKPFQTRAKKSNAS", "aa_hexdigest": "fa8b97cf1baa8f1c4b7da190658b4a16", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI002E1CF638", "query_cov": 0.9333333333333333, "subject_cov": 0.8333333333333334, "identity": 0.586, "score": 83.6, "evalue": 1.39e-19, "valid": false, "product": "50S ribosomal protein L5", "uniref50_id": "UniRef50_A0A2X3HCF5", "kegg_orthology_id": "K02931" }, "pscc": { "uniref50_id": "UniRef50_A0A2X3HCF5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X3HCF5" ], "product": "50S ribosomal protein L5" }, "genes": [], "id": "OAIKOHHJFB_1626", "locus": "GCA000209735_01624" }, { "type": "cds", "sequence": "contig_1", "start": 910187, "stop": 910513, "strand": "-", "frame": 1, "gene": "rplX", "product": "50S ribosomal protein L24", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354770.1", "SO:0001217", "UniParc:UPI0001E32EC1", "UniRef:UniRef100_C4XG04", "UniRef:UniRef50_Q98PZ3", "UniRef:UniRef90_C4XG04" ], "nt": "ATGAAATTAAAACTTAAGAAGAACGACGAAGTTATTGTTATTGCAGGAAGCAATAAAGGTAAAATTGGTCGTATTATTAGAACAAATTTTAAAACAAACCGTGCATATGTAAAAGATGTAAACGTAGTAACTAAACACGTAAAACCAAGTCAAAGTAATACAGAAGGTTCAATCAAGAACGTTGAAGCTCCTATTCACATTTCAAATCTTGCAATCGTTACTAAAAAAGCTACCAAAAGTGGTGCTGCTCAATATTCAAAAGTTGGTTTTAAAGTTAAAGGTAACACTAAAGTTAGAGTTGCTCGTAAAACTAAGAAGGAAATTTAA", "aa": "MKLKLKKNDEVIVIAGSNKGKIGRIIRTNFKTNRAYVKDVNVVTKHVKPSQSNTEGSIKNVEAPIHISNLAIVTKKATKSGAAQYSKVGFKVKGNTKVRVARKTKKEI", "aa_hexdigest": "3e73b247f84e4d4d23a189070ede1090", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI0001E32EC1", "ncbi_nrp_id": "WP_013354770.1", "uniref100_id": "UniRef100_C4XG04", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EC1", "RefSeq:WP_013354770.1", "UniRef:UniRef100_C4XG04" ] }, "ips": { "uniref100_id": "UniRef100_C4XG04", "uniref90_id": "UniRef90_C4XG04" }, "psc": { "uniref90_id": "UniRef90_C4XG04", "gene": "rplX", "product": "50S ribosomal protein L24", "uniref50_id": "UniRef50_Q98PZ3", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PZ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PZ3" ], "product": "Large ribosomal subunit protein uL24" }, "genes": [ "rplX" ], "id": "OAIKOHHJFB_1627", "locus": "GCA000209735_01625" }, { "type": "cds", "sequence": "contig_1", "start": 910535, "stop": 910903, "strand": "-", "frame": 1, "gene": "rplN", "product": "50S ribosomal protein L14", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015934", "KEGG:K02874", "RefSeq:WP_041594261.1", "SO:0001217", "UniParc:UPI0003141A6C", "UniRef:UniRef100_C4XG05", "UniRef:UniRef50_Q85CT4", "UniRef:UniRef90_C4XG05" ], "nt": "ATGGTTATAGCACTTTCTAAATTAAATGTTGCAGATAACTCTGGAGCAAAAGAAGTTGGTGTAATCAAAGTTTTAGGTGGAAGTAGAAAGAAAACAGCTAATATCGGTGACGTTATTATCTGTTCAGTTAAAAAAGCTTTACCTAATGGTTTAGTAAAAGAAGGACAAGTAGTTAAAGGTGTTATTGTTAGAAGTGTTTATGGAATTAGCCGTGAAAATGGTACACATATTCGTTTCGACGATAATGCAGTTGTGTTAATTAAAGATGATGGTACTTTAAGAGGTACTAGTGTGTTTGGACCTGTTGCTCGTGAATTACGTGACCGTGGTTATTTAAAAATCGTTTCATTAGCACCTGAAGTTCTTTAA", "aa": "MVIALSKLNVADNSGAKEVGVIKVLGGSRKKTANIGDVIICSVKKALPNGLVKEGQVVKGVIVRSVYGISRENGTHIRFDDNAVVLIKDDGTLRGTSVFGPVARELRDRGYLKIVSLAPEVL", "aa_hexdigest": "bcb81b9d7c5d7741e3be4807f31816ca", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0003141A6C", "ncbi_nrp_id": "WP_041594261.1", "uniref100_id": "UniRef100_C4XG05", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003141A6C", "RefSeq:WP_041594261.1", "UniRef:UniRef100_C4XG05" ] }, "ips": { "uniref100_id": "UniRef100_C4XG05", "uniref90_id": "UniRef90_C4XG05" }, "psc": { "uniref90_id": "UniRef90_C4XG05", "gene": "rplN", "product": "50S ribosomal protein L14", "uniref50_id": "UniRef50_Q85CT4", "kegg_orthology_id": "K02874", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "genes": [ "rplN" ], "id": "OAIKOHHJFB_1628", "locus": "GCA000209735_01626" }, { "type": "cds", "sequence": "contig_1", "start": 910903, "stop": 911169, "strand": "-", "frame": 2, "gene": "rpsQ", "product": "30S ribosomal protein S17", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354772.1", "SO:0001217", "UniParc:UPI0001E32EC3", "UniRef:UniRef100_C4XG06", "UniRef:UniRef50_Q98PZ1", "UniRef:UniRef90_C4XG06" ], "nt": "ATGGAAAGAAGTTCACGTAAGACCCTTCAAGGTCGTGTTGCTTCAGCACGTGGTGATAAAACTATCATCGTTGAAGTTGAAAGTTATAAAGCTCATAGTCTTTACTCAAAACGTTTTAAAGTTGTTAAACGTTTCGCAGTTCATGATGAAAAAAACTTAGCTAAAGTTAACGACATCGTTACAATTATGGAAACACGTCCTCTTTCAAAAACCAAACACTTCCGTCTTGTTGAAATTAAACAACACGCAGTTGAAGGAGAAAAATAA", "aa": "MERSSRKTLQGRVASARGDKTIIVEVESYKAHSLYSKRFKVVKRFAVHDEKNLAKVNDIVTIMETRPLSKTKHFRLVEIKQHAVEGEK", "aa_hexdigest": "9988ace3ccb6a0572c22a13f19cba37f", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001E32EC3", "ncbi_nrp_id": "WP_013354772.1", "uniref100_id": "UniRef100_C4XG06", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EC3", "RefSeq:WP_013354772.1", "UniRef:UniRef100_C4XG06" ] }, "ips": { "uniref100_id": "UniRef100_C4XG06", "uniref90_id": "UniRef90_C4XG06" }, "psc": { "uniref90_id": "UniRef90_C4XG06", "gene": "rpsQ", "product": "30S ribosomal protein S17", "uniref50_id": "UniRef50_Q98PZ1", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PZ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PZ1" ], "product": "Small ribosomal subunit protein uS17" }, "genes": [ "rpsQ" ], "id": "OAIKOHHJFB_1629", "locus": "GCA000209735_01627" }, { "type": "cds", "sequence": "contig_1", "start": 911169, "stop": 911372, "strand": "-", "frame": 3, "gene": "rpmC", "product": "50S ribosomal protein L29", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354773.1", "SO:0001217", "UniParc:UPI0001E32EC4", "UniRef:UniRef100_C4XG07", "UniRef:UniRef50_A5IYX8", "UniRef:UniRef90_C4XG07" ], "nt": "ATGCTTTATAAAGATATTAAAGTTAAAAATAATGCTGAACTCCGTAAATTACTTGACGATTTAAAAGCACAATTATTTATTTATCGTTTCCAAAACAAAACTGGGACATTAGATCACAGTCACAAAATTAGAGAAGTACGTCGTGACATTGCTAAAGTATTAACAGTTTTAAAAATTAACGAATCTAAAGGAGGAGCTAAATAA", "aa": "MLYKDIKVKNNAELRKLLDDLKAQLFIYRFQNKTGTLDHSHKIREVRRDIAKVLTVLKINESKGGAK", "aa_hexdigest": "37e9c9e6d745504fa848d9e6f84a8d70", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001E32EC4", "ncbi_nrp_id": "WP_013354773.1", "uniref100_id": "UniRef100_C4XG07", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EC4", "RefSeq:WP_013354773.1", "UniRef:UniRef100_C4XG07" ] }, "ips": { "uniref100_id": "UniRef100_C4XG07", "uniref90_id": "UniRef90_C4XG07" }, "psc": { "uniref90_id": "UniRef90_C4XG07", "gene": "rpmC", "product": "50S ribosomal protein L29", "uniref50_id": "UniRef50_A5IYX8", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A5IYX8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IYX8" ], "product": "Large ribosomal subunit protein uL29" }, "genes": [ "rpmC" ], "id": "OAIKOHHJFB_1630", "locus": "GCA000209735_01628" }, { "type": "cds", "sequence": "contig_1", "start": 911516, "stop": 911623, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00252.24" ], "nt": "ATGGGTCGTGAAGGTCAAGTTTTCATTAGAATCTTCCCTCACTTCTCAAAAACATCAAAACCTATCGGTGTTCGTATGGGTTCTGGTAAAGGTAGTCCTGAAAAATGA", "aa": "MGREGQVFIRIFPHFSKTSKPIGVRMGSGKGSPEK", "aa_hexdigest": "697d90d1d37e401998077d88652ea801", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00252.24", "name": "Ribosomal_L16", "length": 32, "aa_cov": 0.9142857142857143, "hmm_cov": 1.0, "evalue": 2.6611707011868143e-10, "score": 36.4363899230957, "start": 3, "stop": 34 } ], "seq_stats": { "molecular_weight": 3847.4728000000005, "isoelectric_point": 11.068786430358884 }, "id": "OAIKOHHJFB_1631", "locus": "GCA000209735_01629" }, { "type": "cds", "sequence": "contig_1", "start": 911672, "stop": 911797, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTCAACCAAAAAGAACTAAATATAGAAAACCATTTTTAGTACGTCATGACAAACGTAAAGCCTTCAAAGGTAATACAGTTGCTTATGGTGACTTTGGTTTACAAGCTGTAAGCTCAGCTTGA", "aa": "MLQPKRTKYRKPFLVRHDKRKAFKGNTVAYGDFGLQAVSSA", "aa_hexdigest": "a28e4943ebc9e731e4abe2f6b2bfc039", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4711.454900000001, "isoelectric_point": 10.74824962615967 }, "id": "OAIKOHHJFB_1632", "locus": "GCA000209735_01630" }, { "type": "cds", "sequence": "contig_1", "start": 911835, "stop": 911954, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00189.25" ], "nt": "ATGGCTAGAACAGAAGGCTATTCACGTGGAGAAATGAGACTTCACACATTAAGACAAGATGTTGATTATGCACACGCAAATGCTCACACTACTTATGGTGTAATCGGCGTTAAAGTGTGA", "aa": "MARTEGYSRGEMRLHTLRQDVDYAHANAHTTYGVIGVKV", "aa_hexdigest": "6bec5ed16b8584a907b907b62f925a05", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00189.25", "name": "Ribosomal_S3_C", "length": 39, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 4.335491738740595e-15, "score": 52.15079879760742, "start": 1, "stop": 39 } ], "seq_stats": { "molecular_weight": 4404.901, "isoelectric_point": 8.247198677062986 }, "id": "OAIKOHHJFB_1633", "locus": "GCA000209735_01631" }, { "type": "cds", "sequence": "contig_1", "start": 912456, "stop": 912815, "strand": "-", "frame": 3, "gene": "rplV", "product": "50S ribosomal protein L22", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015934", "KEGG:K02890", "RefSeq:WP_013354776.1", "SO:0001217", "UniParc:UPI0001E32EC7", "UniRef:UniRef100_C4XG10", "UniRef:UniRef50_Q98PY6", "UniRef:UniRef90_C4XG10" ], "nt": "ATGGCACAAAATACACCAGTTGTTCAACAAGCTAAAGCTCATGTTGAAATTCAACGTGTTAGTGCACGTAAAGCACGTTTAGTTGCTGACTTAGTTAGATCTAAAGACGTGCGTGAAGCTCTTGGTATTTTAAGAAATACAAACAAGAAATCTTCAGAATTATTTATTAAACTTATTAACTCAGCAATTGCTAATGCTACAAACAACCACGGAATGGATGCATCTAAATTATTTGTTAAAGAAATCTTAGTTAATGAAGGACCAACACTTAAAAGATACCAACCTCACTCAAGAGGAACAGCTTATTCAATTTTTAAACGTACATCACATTTATCAATTACATTAGAAGAAAGAAAGTAG", "aa": "MAQNTPVVQQAKAHVEIQRVSARKARLVADLVRSKDVREALGILRNTNKKSSELFIKLINSAIANATNNHGMDASKLFVKEILVNEGPTLKRYQPHSRGTAYSIFKRTSHLSITLEERK", "aa_hexdigest": "eb44942af9594e8c854873f987f7fb42", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001E32EC7", "ncbi_nrp_id": "WP_013354776.1", "uniref100_id": "UniRef100_C4XG10", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E32EC7", "RefSeq:WP_013354776.1", "UniRef:UniRef100_C4XG10" ] }, "ips": { "uniref100_id": "UniRef100_C4XG10", "uniref90_id": "UniRef90_C4XG10" }, "psc": { "uniref90_id": "UniRef90_C4XG10", "gene": "rplV", "product": "50S ribosomal protein L22", "uniref50_id": "UniRef50_Q98PY6", "kegg_orthology_id": "K02890", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PY6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PY6" ], "product": "Large ribosomal subunit protein uL22" }, "genes": [ "rplV" ], "id": "OAIKOHHJFB_1634", "locus": "GCA000209735_01632" }, { "type": "cds", "sequence": "contig_1", "start": 912989, "stop": 913093, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTCGTAGTCTTAAAAAAGGACCTTTTGCAGACGAACATTTACTTAAAAAAGTAGATGCTATTGTTGAAGGCAAAGCGCCAAAAAAACCAATTAAAACTTGA", "aa": "MARSLKKGPFADEHLLKKVDAIVEGKAPKKPIKT", "aa_hexdigest": "159a49d0b0c41ac497ccc03212c29f2d", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3745.4823000000015, "isoelectric_point": 9.998095512390137 }, "id": "OAIKOHHJFB_1635", "locus": "GCA000209735_01633" }, { "type": "cds", "sequence": "contig_1", "start": 913180, "stop": 913938, "strand": "-", "frame": 2, "gene": "rplB", "product": "50S ribosomal protein L2", "db_xrefs": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740", "KEGG:K02886", "SO:0001217", "UniRef:UniRef50_Q9AMK8", "UniRef:UniRef90_D5E4V9" ], "nt": "ATGGCAATTAAACATTACAAGCCAACTACGAATGGACGTCGTAATATGTCATCTCTACATTACACAGAAAACTTAAGTGGACATGCTCCTGAAAAATCTTTACTTACACTATTAAAATATAGTGCTGGTCGCAATAACCAAGGTAAGATTACAGTACGTCACAAGGGTGGTCGTGTTAAGAGATTTTACAGATTAATTGACTTCAAACGTAATAAGGACAATATTCCAGCAGTAGTTAAGACCATTGAATATGATCCTAACCGTTCAGCTAATATTTGTTTATTAGCATATGCTGATGGAGAAAAAAGGTATATTTTAGCGCCTGAAGGCATTAAAGTAGGCCAAAGCGTTATTTCAGGTGAACACGCTGACATTTTAGTAGGTAATGCATTACCACTTTCAAGCATTCCAGAAGGTACAATTGTACACAACATTGAAATGCAACCAGGTGGTGGAGGTATTATTGCTAGATCAGCAGGTACATCTGCACAAATCTTAGGTAAAGATGACGACGGTAAATATGTTGTTTTACGTCTAAAAAGCGGTGAAATGCGTCGTATCTTAGCTCGTTGCCGTGCAACTATCGGTTCAGTTGGTAACGAAGAACATTTATTGGTTGATTTAGGAAAAGCTGGTAGAAACCGTTATAAAGGTGTTCGTCCAACAGTTCGTGGTTCAGTTATGAACCCTGTGGATCACCCACATGGTGGTGGTGAAGGTAAACAACCTGTTGGTCGTAAAGCTCCTCTTACTCCTTGA", "aa": "MAIKHYKPTTNGRRNMSSLHYTENLSGHAPEKSLLTLLKYSAGRNNQGKITVRHKGGRVKRFYRLIDFKRNKDNIPAVVKTIEYDPNRSANICLLAYADGEKRYILAPEGIKVGQSVISGEHADILVGNALPLSSIPEGTIVHNIEMQPGGGGIIARSAGTSAQILGKDDDGKYVVLRLKSGEMRRILARCRATIGSVGNEEHLLVDLGKAGRNRYKGVRPTVRGSVMNPVDHPHGGGEGKQPVGRKAPLTP", "aa_hexdigest": "054fb9374afee5cadbbdf737004e8920", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_D5E4V9", "query_cov": 1.0, "subject_cov": 0.8904593639575972, "identity": 1.0, "score": 503.0, "evalue": 1.17e-179, "valid": true, "gene": "rplB", "product": "50S ribosomal protein L2", "uniref50_id": "UniRef50_Q9AMK8", "kegg_orthology_id": "K02886", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740" ] }, "pscc": { "uniref50_id": "UniRef50_Q9AMK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9AMK8" ], "product": "Large ribosomal subunit protein uL2" }, "genes": [ "rplB" ], "id": "OAIKOHHJFB_1636", "locus": "GCA000209735_01634" }, { "type": "cds", "sequence": "contig_1", "start": 914010, "stop": 914288, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTACTTTAGCTGAAGGAGATAAAATCGTTTATTATCCAAATGAAGCTGAAGCTCAAGATGCTAAAAAAGAAGCTAAAGTTAAAGCTAAAAAAGCTGAAGATAAAAAACAAGCAGCAGCTAAAGAAGCAGAAATTGCTAAAAAACTTGCTGCTAAAAAAGCTAAAGCAGCTGAAAGCAAAAAAGCAGACAGCTCAAAAGCTGAAAAAACTACAAAATCAACTACTTCAAAACCTGCTGCTAAAAAAGTTGCAGCTGCTAAGAAGAGTTCAAAATAA", "aa": "MITLAEGDKIVYYPNEAEAQDAKKEAKVKAKKAEDKKQAAAKEAEIAKKLAAKKAKAAESKKADSSKAEKTTKSTTSKPAAKKVAAAKKSSK", "aa_hexdigest": "792aa382020172fe834ae68c3dcba999", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9736.16699999999, "isoelectric_point": 9.875154304504395 }, "id": "OAIKOHHJFB_1637", "locus": "GCA000209735_01635" }, { "type": "cds", "sequence": "contig_1", "start": 914582, "stop": 914755, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAAAATCTCAACTAAAGATGCTGTTAACAAACTTAAATCATTAAAAATTGAAAACTTAAAACACATTTTAGTGGTAACAACAGATGAAACTGTTTACAAATCAACAGCTAACTTACAAAATGTTTTATGTGTTAAACCAAACTCAGTAAGTGTTGAAAACCTTGTTTGA", "aa": "MSKISTKDAVNKLKSLKIENLKHILVVTTDETVYKSTANLQNVLCVKPNSVSVENLV", "aa_hexdigest": "f17e344e8b3a504831f257ea37f66ff9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6299.3160000000025, "isoelectric_point": 9.25226078033447 }, "id": "OAIKOHHJFB_1638", "locus": "GCA000209735_01636" }, { "type": "cds", "sequence": "contig_1", "start": 914951, "stop": 915655, "strand": "-", "frame": 1, "gene": "rplD", "product": "50S ribosomal protein L4", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0022625", "SO:0001217", "UniRef:UniRef50_C4XG14", "UniRef:UniRef90_C4XG14" ], "nt": "ATGGCTGAAACAAAGAAAAAAGCTACTACTAAAGCTAGTGAAGAAAAGAAAACTATAACTAAAAAAGTAGCAACTGCTAAGAAAACTACTACTGCTAAATCAACTTCTACAGCTAAAAAATCAACAACTACTGCTAAAAAAGCTACTACAACAACTAAAAAATCCGCTGCTCCTAAAGCAACAGCTGAAAAGAAAACAACTTCAAGAAAAAAAGTTACAGTTGAAATTGATTTAACTAAAAACTTAAAACCTAAAAAGCCTACTAAAGCTGAATTAAAAGCTCAAGAAGCTAAAGAAGAAAGTGCTAAAAAGAAAACCGCTGCTAAAACAGCTGCTCAAAAAGCTAAATTAGCTAAAGAAGAAGCTGCTGCAAAAGCAAAAGCAGAAAAAGAAGCACAAGCAGCTTTAGATGAATTAAAAGCTGCAGCAGAAGCTGCTAAAAGAAAAGCTAAAACTTATGCATCTTCAAGAGCAAAAGTTGCTTCACTTAAATTAAATGATGCTAAACTTGAACAAGAAAAATTTACAAAAGTTATCTTGCTTCACTTTGACAATAAAGCCTTACCAAAAGAATTATTTGCATCACCAAAAATTTATGAACAAGCAATTTTTGACACCATTATGGCTGATAGAGCATCAAGACGTCAAGGTACTCATGATGTTAAAAATCGTTCAGAAGTAAGCGGTGGTGGTAAAAAACCTTGA", "aa": "MAETKKKATTKASEEKKTITKKVATAKKTTTAKSTSTAKKSTTTAKKATTTTKKSAAPKATAEKKTTSRKKVTVEIDLTKNLKPKKPTKAELKAQEAKEESAKKKTAAKTAAQKAKLAKEEAAAKAKAEKEAQAALDELKAAAEAAKRKAKTYASSRAKVASLKLNDAKLEQEKFTKVILLHFDNKALPKELFASPKIYEQAIFDTIMADRASRRQGTHDVKNRSEVSGGGKKP", "aa_hexdigest": "9ae2affe01022b21dd6220ad6a3783ba", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "rplD" ], "truncated": "5-prime", "pseudogene": { "start": 914648, "stop": 915658, "observations": { "mutation": [ 914899, 914953 ], "stop": [ 914899, 914953 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG14", "query_cov": 1.0, "subject_cov": 0.6190476190476191, "identity": 1.0, "score": 215.0, "evalue": 4.45e-65, "gene_start": 1, "gene_end": 234, "reference_start": 2, "reference_end": 235, "reference_sequence": "MMAETKKKATTKASEEKKTITKKVATAKKTTTAKSTSTAKKSTTTAKKATTTTKKSAAPKATAEKKTTSRKKVTVEIDLTKNLKPKKPTKAELKAQEAKEESAKKKTAAKTAAQKAKLAKEEAAAKAKAEKEAQAALDELKAAAEAAKRKAKTYASSRAKVASLKLNDAKLEQEKFTKVILLHFDNKALPKELFASPKIYEQAIFDTIMADRASRRQGTHDVKNRSEVSGGGKKPWRQKGTGRARAGSTRSPIWVGGGRAFGPTPERNYSLKVNKKVRRAAFLSALTLLSNKKSVVIDDFKMSKISTKDAVNKLKSLKIENLKHILVVTTDETVYKSTANLQNVLCVKPNSVSVENLVWADVLVLSNEGLKNFEGRIK" }, "paralog": false, "identity": 0.9880952380952381, "score": 410.0, "evalue": 3.53e-144, "description": "Internal stop codon at 914899, 914953. Nonsense mutation around 914899, 914953.", "psc": { "uniref90_id": "UniRef90_C4XG14", "gene": "rplD", "product": "50S ribosomal protein L4", "uniref50_id": "UniRef50_C4XG14", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0022625" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG14", "UniRef:UniRef50_C4XG14", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG14" ], "product": "Large ribosomal subunit protein uL4" } }, "id": "OAIKOHHJFB_1639", "locus": "GCA000209735_01637" }, { "type": "cds", "sequence": "contig_1", "start": 915655, "stop": 916002, "strand": "-", "frame": 2, "gene": "rplC", "product": "50S ribosomal protein L3", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02906", "SO:0001217", "UniRef:UniRef50_Q98PY0", "UniRef:UniRef90_C4XG15" ], "nt": "ATGCCAGGTCACTTAGGAGCTGTTAGAACAACAGTTCAAAATCTTGAAATTGTTAAAGTTGATGTAGCAAACAACATTATGTTAGTTAAAGGATCAATTCCTGGGGCTAAAAATTCATATGTTATGATTCGTGAAGCTGTTAAAGGCTTACCAAATCATGATGCAATTAAACTTGTTGATATTAAAGAAGTTCTTAAAATGAATGAATTGCTTGAAAGAGCTAAAAAATACAACATTGAAGTTACAGTTGGTATGCATTCAAGTGAATTAGAACCTCTTATCCAAAAAGCTGAAGCTGATGCAGCTGCAGCAGCTGAAGAAAGTAAAAAAGCAGAAGGAGATAAATAA", "aa": "MPGHLGAVRTTVQNLEIVKVDVANNIMLVKGSIPGAKNSYVMIREAVKGLPNHDAIKLVDIKEVLKMNELLERAKKYNIEVTVGMHSSELEPLIQKAEADAAAAAEESKKAEGDK", "aa_hexdigest": "acab8c86c449961495c19a7a3340cc7a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "rplC" ], "truncated": "3-prime", "pseudogene": { "start": 915655, "stop": 916302, "observations": { "mutation": [ 916116 ], "stop": [ 916116 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG15", "query_cov": 1.0, "subject_cov": 0.4166666666666667, "identity": 1.0, "score": 212.0, "evalue": 2.72e-67, "gene_start": 1, "gene_end": 115, "reference_start": 162, "reference_end": 276, "reference_sequence": "MMKGILGRKIGMTQIFTELGASVPVTVIEVQPNVVTKVLSADKNGYVATQIAVGEKKEHRSNKPLKGQFAQAKTTPKRYVKEIRGMQGYELGQTIKANIFKAGELVDITGTSKGKGFAGTIKRWNQHIGPKSHGGGGGSQPVRQTGSLGDISGNRVFKGMTMPGHLGAVRTTVQNLEIVKVDVANNIMLVKGSIPGAKNSYVMIREAVKGLPNHDAIKLVDIKEVLKMNELLERAKKYNIEVTVGMHSSELEPLIQKAEADAAAAAEESKKAEGDK" }, "paralog": false, "identity": 0.9953488372093023, "score": 420.0, "evalue": 1.92e-151, "description": "Internal stop codon at 916116. Nonsense mutation around 916116.", "psc": { "uniref90_id": "UniRef90_C4XG15", "gene": "rplC", "product": "50S ribosomal protein L3", "uniref50_id": "UniRef50_Q98PY0", "kegg_orthology_id": "K02906", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG15", "UniRef:UniRef50_Q98PY0", "KEGG:K02906", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PY0" ], "product": "Large ribosomal subunit protein uL3" } }, "id": "OAIKOHHJFB_1640", "locus": "GCA000209735_01638" }, { "type": "cds", "sequence": "contig_1", "start": 916114, "stop": 916482, "strand": "-", "frame": 2, "gene": "rplC", "product": "50S ribosomal protein L3", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02906", "SO:0001217", "UniRef:UniRef50_Q98PY0", "UniRef:UniRef90_C4XG15" ], "nt": "ATGAAAGGAATCTTAGGACGGAAGATTGGTATGACACAAATCTTTACTGAACTTGGAGCTAGTGTTCCAGTTACAGTTATAGAAGTGCAACCAAATGTTGTTACTAAAGTTCTTTCAGCTGACAAAAATGGGTATGTTGCAACCCAAATTGCTGTTGGTGAAAAGAAAGAACACAGAAGCAATAAACCTCTTAAAGGTCAATTTGCTCAAGCTAAAACAACACCTAAGCGCTACGTTAAAGAAATTCGTGGCATGCAAGGCTATGAATTAGGTCAAACTATTAAGGCCAACATTTTTAAAGCTGGTGAATTAGTAGATATCACTGGTACATCAAAAGGTAAAGGTTTTGCCGGTACAATCAAAAGATGA", "aa": "MKGILGRKIGMTQIFTELGASVPVTVIEVQPNVVTKVLSADKNGYVATQIAVGEKKEHRSNKPLKGQFAQAKTTPKRYVKEIRGMQGYELGQTIKANIFKAGELVDITGTSKGKGFAGTIKR", "aa_hexdigest": "5cd896c66477f169406bf9b1ac3518b2", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "rplC" ], "truncated": "5-prime", "pseudogene": { "start": 915811, "stop": 916485, "observations": { "mutation": [ 916116 ], "stop": [ 916116 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG15", "query_cov": 1.0, "subject_cov": 0.4420289855072464, "identity": 1.0, "score": 233.0, "evalue": 2.33e-75, "gene_start": 1, "gene_end": 122, "reference_start": 2, "reference_end": 123, "reference_sequence": "MMKGILGRKIGMTQIFTELGASVPVTVIEVQPNVVTKVLSADKNGYVATQIAVGEKKEHRSNKPLKGQFAQAKTTPKRYVKEIRGMQGYELGQTIKANIFKAGELVDITGTSKGKGFAGTIKRWNQHIGPKSHGGGGGSQPVRQTGSLGDISGNRVFKGMTMPGHLGAVRTTVQNLEIVKVDVANNIMLVKGSIPGAKNSYVMIREAVKGLPNHDAIKLVDIKEVLKMNELLERAKKYNIEVTVGMHSSELEPLIQKAEADAAAAAEESKKAEGDK" }, "paralog": false, "identity": 0.9910714285714286, "score": 437.0, "evalue": 3.57e-158, "description": "Internal stop codon at 916116. Nonsense mutation around 916116.", "psc": { "uniref90_id": "UniRef90_C4XG15", "gene": "rplC", "product": "50S ribosomal protein L3", "uniref50_id": "UniRef50_Q98PY0", "kegg_orthology_id": "K02906", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG15", "UniRef:UniRef50_Q98PY0", "KEGG:K02906", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PY0" ], "product": "Large ribosomal subunit protein uL3" } }, "id": "OAIKOHHJFB_1641", "locus": "GCA000209735_01639" }, { "type": "cds", "sequence": "contig_1", "start": 916554, "stop": 916859, "strand": "-", "frame": 3, "gene": "rpsJ", "product": "30S ribosomal protein S10", "db_xrefs": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_013354782.1", "SO:0001217", "UniParc:UPI0001A8C186", "UniRef:UniRef100_C4XG16", "UniRef:UniRef50_Q98PX9", "UniRef:UniRef90_C4XG16" ], "nt": "ATGAAAAAAATCAATATCAAGGTTAAAAGTTTCGATCATGCTTTAGTTGATTTTGGAGCAAAGAAAATTGTTGAAGTTGCTACCAAAAACTCAGTTAAAGTAAGTGGTCCAGTTCCTCTTCCAACAAAAAGAGAAGTTGTGACTATCTTAAGATCAGTTCACGTTAACAAAAAATCTCGTGAACAATTTGAATCAAGAACTTTTCAACGTTTGATCGTTTTATTAAACCCAAACGATAAAGCTCTTGACCAAATTAAGAGATTAGAACTTCCAGCAGGTGTTGAAGTTCAAATCTCAACAAAATAA", "aa": "MKKINIKVKSFDHALVDFGAKKIVEVATKNSVKVSGPVPLPTKREVVTILRSVHVNKKSREQFESRTFQRLIVLLNPNDKALDQIKRLELPAGVEVQISTK", "aa_hexdigest": "15182ac8da1878398a66916b08ae983e", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001A8C186", "ncbi_nrp_id": "WP_013354782.1", "uniref100_id": "UniRef100_C4XG16", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C186", "RefSeq:WP_013354782.1", "UniRef:UniRef100_C4XG16" ] }, "ips": { "uniref100_id": "UniRef100_C4XG16", "uniref90_id": "UniRef90_C4XG16" }, "psc": { "uniref90_id": "UniRef90_C4XG16", "gene": "rpsJ", "product": "30S ribosomal protein S10", "uniref50_id": "UniRef50_Q98PX9", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PX9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PX9" ], "product": "Small ribosomal subunit protein uS10" }, "genes": [ "rpsJ" ], "id": "OAIKOHHJFB_1642", "locus": "GCA000209735_01640" }, { "type": "cds", "sequence": "contig_1", "start": 917489, "stop": 917941, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAGAAATTATTCTTTAATTTTTCATTGTTATCTTCTGTAGCTCTACCACTTACAACTATTGCAGCTAGTTGTAATCAAAACTCAGATCAAAATATACCTAACATTACAGTTAGCGCTAGTGAAAAAGGTTTAGGCTACTATCCAAAAACAATACAAAGATTAATTAAAGGCAAAAAAAATAAAGAATTAATTGATACTATTAATCAATTTATAAATAATAAAGAACAAATTAAATATGAAAATAATAAAGAATATTCTTTAAATGAAAACGAAAGTACATTTGGTTACAATGAATTAAAAAAAGAGTATAAATGCATTATTGCATTAAATATTAAAGACAATGACAAAACTAAAGTTAAAAAATTTTCTATTAACAAAATTGGTCACAATAATGTTTATCATGATGATATACACAAATTTGATAGACTTGAGAAAGTTTACTTTTGA", "aa": "MKKKLFFNFSLLSSVALPLTTIAASCNQNSDQNIPNITVSASEKGLGYYPKTIQRLIKGKKNKELIDTINQFINNKEQIKYENNKEYSLNENESTFGYNELKKEYKCIIALNIKDNDKTKVKKFSINKIGHNNVYHDDIHKFDRLEKVYF", "aa_hexdigest": "64dcfc6ace6f2d782e9f968980b0b097", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17476.774599999997, "isoelectric_point": 9.345546531677247 }, "id": "OAIKOHHJFB_1643", "locus": "GCA000209735_01641" }, { "type": "cds", "sequence": "contig_1", "start": 918407, "stop": 918634, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATCAAAAGACTTTTTGGATTTACTAGATAAGGTTGATAAAGAACATAATCATGAACCAAAAGACATAATTAATTTTTTAAAACAATATGTAAATATTGATGGTACTTATGATGCAAATGATAAATATGAATATGCTTTTAACGTAGAAAAAACTCATACTCATGGTTCAGGCCAGTTGCACTTATATACTTACTACCGTGAAAAAGGTAAAAACCAAAAATGA", "aa": "MTSKDFLDLLDKVDKEHNHEPKDIINFLKQYVNIDGTYDANDKYEYAFNVEKTHTHGSGQLHLYTYYREKGKNQK", "aa_hexdigest": "137ca94b2b95e6cad4d06177a5b7958a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8954.7844, "isoelectric_point": 6.165973472595215 }, "id": "OAIKOHHJFB_1644", "locus": "GCA000209735_01642" }, { "type": "cds", "sequence": "contig_1", "start": 918680, "stop": 918895, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCGGATTAAAATTCAATAATGCTGGTATTAAAGACGCAAAACAAGCAAATGAAGATCCAGGATTTTACACAAGTCCACATCAAATACTTAGAGAAATGGAAACCAAATCTACATTTGATGAACAACTAGCAGTATTGAAAAAATATTGTGAAGTTAAAGACGAATCAGATAAAAATAAATATGACTACAAATTCAATTTTAAAGAATCATGA", "aa": "MGGLKFNNAGIKDAKQANEDPGFYTSPHQILREMETKSTFDEQLAVLKKYCEVKDESDKNKYDYKFNFKES", "aa_hexdigest": "49406b5a7f89a963e2a6e0704ca5ebec", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8260.130999999998, "isoelectric_point": 5.769635200500488 }, "id": "OAIKOHHJFB_1645", "locus": "GCA000209735_01643" }, { "type": "cds", "sequence": "contig_1", "start": 919718, "stop": 919849, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGACTTGATTTAAAAAACTTTGGAGCATTTTCTAAAGTTGGCGATTTTTTAATAAAAAATACACCTTCAGAAACGGATATTGTTAATGTTCGTGCTCAAGACATTATAAATAAAGCTAAAAGTATTTGA", "aa": "MRLDLKNFGAFSKVGDFLIKNTPSETDIVNVRAQDIINKAKSI", "aa_hexdigest": "4722c44c811fc246110dd46ef1c40979", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4808.513800000001, "isoelectric_point": 9.397443580627442 }, "id": "OAIKOHHJFB_1646", "locus": "GCA000209735_01644" }, { "type": "cds", "sequence": "contig_1", "start": 919925, "stop": 920098, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTTTCCCAGGATATGATAATACTGTTATTAATGAAGATTTAGAATACACAATTGATTTCAATAAAGCTGTTGTATATAAAAGTGGAAAATTAGAATTGAACATTAAAGTAAAGAACACTAAAACAAATGAAGAAGAAAATGTTTATATTCTATTTTGCAAATTAGCCTAA", "aa": "MGFPGYDNTVINEDLEYTIDFNKAVVYKSGKLELNIKVKNTKTNEEENVYILFCKLA", "aa_hexdigest": "51ce64c015467534b7f401ad78473eaf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6578.4301, "isoelectric_point": 4.893632698059082 }, "id": "OAIKOHHJFB_1647", "locus": "GCA000209735_01645" }, { "type": "cds", "sequence": "contig_1", "start": 920840, "stop": 921727, "strand": "+", "frame": 2, "gene": "recA", "product": "recombinase RecA", "db_xrefs": [ "GO:0003677", "GO:0003697", "GO:0005524", "GO:0006281", "GO:0008094", "KEGG:K03553", "SO:0001217", "UniRef:UniRef50_Q07809", "UniRef:UniRef90_C4XG20" ], "nt": "ATGAAAAGCAAAAATATTGAAATTAAAAATAAAGACAAAGAATTAAAAGAAGCTTTAGAAGAAATTACTAAAAAATTTGGATCTGAAGCAATTATGCTTTTTGGACAAAATGAAATTGAAGAAAAAATTGAAACTTTTCCAAGCGGTTCATATCTTTTAGATAATGCACTTGGTATTGGCGGTTATCCAAAAGGAAGAATTATTGAAATTTATGGACCTGAAAGCAGTGGTAAAACCACACTTTGTTTACATGCAATTGCCGAAATTCAAAAACAGGGAGGAATAGCAGCTTATATTGATGCTGAGCACTCACTTGATCCAAATTATGCTCGCAATTTAGGAATTAATCTTGATAACTTAATACTTTCTCAACCTGATAGTGGAGAACAAGCTTTACAAATAGTCGATATATTAGCTAAAAGCGGTTCTATTGATTTAATTGTTGTTGATAGTGTTGCTGCATTAGTACCTGAAGTAGAATTAAATGGAACAATGAGCGATTCTTCTGTCGGAGCTTTAGCTCGTTTAATGGCTAAAGGTTTAAGAAAAATAACTGGTAATTTAAACAAAAATAAAACAGCAATTATTTTTGTAAACCAAATTAGAGAAAAAATTGGTGTTTTATTTGGCAATCCAGAAACAACAACTGGAGGCCGAGCAATTAAATTCTTCTCATCAGTTAGAATTGAAGTAAGAAAAACCACATCAATTGTAGAAGGAAAAGACATAACAGGAAATGAAGTTAAAATCAAAATAGTTAAAAACAAATTAGCTGCTCCTTATAAATCATTTACCACTGAAATTATTTTTTCTAAAGGAATCGACAAGTTAAATGAGTTAATTAATTTAGCTACAGAAGCAAATGTTATTGAGAAAAAAGGTGCTTGA", "aa": "MKSKNIEIKNKDKELKEALEEITKKFGSEAIMLFGQNEIEEKIETFPSGSYLLDNALGIGGYPKGRIIEIYGPESSGKTTLCLHAIAEIQKQGGIAAYIDAEHSLDPNYARNLGINLDNLILSQPDSGEQALQIVDILAKSGSIDLIVVDSVAALVPEVELNGTMSDSSVGALARLMAKGLRKITGNLNKNKTAIIFVNQIREKIGVLFGNPETTTGGRAIKFFSSVRIEVRKTTSIVEGKDITGNEVKIKIVKNKLAAPYKSFTTEIIFSKGIDKLNELINLATEANVIEKKGA", "aa_hexdigest": "60b1b70bcf4cd495875ccf9d0878ed77", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_C4XG20", "query_cov": 1.0, "subject_cov": 0.8651026392961877, "identity": 1.0, "score": 558.0, "evalue": 8.98e-200, "valid": true, "gene": "recA", "product": "recombinase RecA", "uniref50_id": "UniRef50_Q07809", "kegg_orthology_id": "K03553", "go_ids": [ "GO:0003677", "GO:0003697", "GO:0005524", "GO:0006281", "GO:0008094" ] }, "pscc": { "uniref50_id": "UniRef50_Q07809", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q07809" ], "product": "Protein RecA" }, "genes": [ "recA" ], "id": "OAIKOHHJFB_1648", "locus": "GCA000209735_01646" }, { "type": "cds", "sequence": "contig_1", "start": 922082, "stop": 922249, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG21", "UniRef:UniRef50_UPI000FF82D94", "UniRef:UniRef90_C4XG21", "UniRef:UniRef90_UPI000FF82D94" ], "nt": "ATGATTAAAATTGCTGAATTGTTAAAAGTCCAAATAGAAGATGTTAGAAACAATTATCAAAACTTTTTAGGCTTGTTTAGCGAAGTAAGACTAAGAGTTAGATTATGCAGAAAAGAAAACATGCATTTTAGTGATGAATTACAAATAGATGCTTGTATCCATGAATGA", "aa": "MIKIAELLKVQIEDVRNNYQNFLGLFSEVRLRVRLCRKENMHFSDELQIDACIHE", "aa_hexdigest": "f8f53ede93574aece135216a50673eb5", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI000FF82D94", "query_cov": 1.0, "subject_cov": 0.8333333333333334, "identity": 0.909, "score": 103.0, "evalue": 3.08e-28, "valid": true, "uniref50_id": "UniRef50_UPI000FF82D94" }, "pscc": { "uniref50_id": "UniRef50_UPI000FF82D94", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FF82D94" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 921983, "stop": 922249, "observations": { "mutation": [ 921997, 922084 ], "stop": [ 921997, 922084 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG21", "query_cov": 1.0, "subject_cov": 0.625, "identity": 0.982, "score": 112.0, "evalue": 1.76e-31, "gene_start": 1, "gene_end": 55, "reference_start": 1, "reference_end": 55, "reference_sequence": "MIKIAELLKVQIEDVRNNYKNFLGLFSEVRLRVRLCRKENMHFSDELQIDACIHEWFDGTKNAYYHAIDVAIIIIYFFAFVFYIWFRY" }, "paralog": false, "identity": 0.9659090909090909, "score": 172.0, "evalue": 1.73e-57, "description": "Internal stop codon at 921997, 922084. Nonsense mutation around 921997, 922084.", "psc": { "uniref90_id": "UniRef90_C4XG21", "uniref50_id": "UniRef50_C4XG21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG21", "UniRef:UniRef50_C4XG21" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG21" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6619.632799999998, "isoelectric_point": 6.0718481063842775 }, "id": "OAIKOHHJFB_1649", "locus": "GCA000209735_01647" }, { "type": "cds", "sequence": "contig_1", "start": 922810, "stop": 923040, "strand": "+", "frame": 1, "gene": null, "product": "Metallophosphoesterase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBC5" ], "nt": "ATGACAAAAGAAAACAAAACTTTAAATATTCTTTTTATTGGGGATATTTTTTCTGAAGCAGGAATTGAAATAGTTGAAAAACATTTACCTTCTTTAATAAAAGAAAATAAAATCGATGTTGTTATAGCGCAAAGTGAAAATGTTTCAGGAAGAAAAGGTTTAGTTAAAAAAGATTATTTAAGACTTAAAAAAGCAGGTGTTAATATTTTTACAATTGGAAACCATGTTTGA", "aa": "MTKENKTLNILFIGDIFSEAGIEIVEKHLPSLIKENKIDVVIAQSENVSGRKGLVKKDYLRLKKAGVNIFTIGNHV", "aa_hexdigest": "2a3296f3a3bd25315eab08a421cf3e34", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A432FBC5", "query_cov": 0.9078947368421053, "subject_cov": 1.0, "identity": 0.58, "score": 89.7, "evalue": 3.53e-22, "valid": false, "product": "Metallophosphoesterase", "uniref50_id": "UniRef50_A0A432FBC5" }, "pscc": { "uniref50_id": "UniRef50_A0A432FBC5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBC5" ], "product": "Metallophosphoesterase" }, "genes": [], "id": "OAIKOHHJFB_1650", "locus": "GCA000209735_01648" }, { "type": "cds", "sequence": "contig_1", "start": 923383, "stop": 923628, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13277.11" ], "nt": "GTGCAAACTAATGATGCACATGTGCTACCTAAGGGGACTTGTTATTTAACAGATGCTGGAATGAGTGGGCCATTTGATTGTGCTATTGGAGCAAACTTTGATGAAGTTTATCAAAAAATGAGATATAACAAGAATGTTAAATTCATTCCTTCAAAAAACAAAAGCCAATTTAATGGTGCTATATTAAAACTAAATAGTGACAAAAGCCAAAACAAAATTATTCCAATTAACATAAGACAAGAATAA", "aa": "MQTNDAHVLPKGTCYLTDAGMSGPFDCAIGANFDEVYQKMRYNKNVKFIPSKNKSQFNGAILKLNSDKSQNKIIPINIRQE", "aa_hexdigest": "9d9c00019db0b50b5a1ddd9eecb21784", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13277.11", "name": "YmdB", "length": 73, "aa_cov": 0.8888888888888888, "hmm_cov": 1.0, "evalue": 1.2835316716422659e-18, "score": 63.27640914916992, "start": 2, "stop": 73 } ], "seq_stats": { "molecular_weight": 9110.331299999996, "isoelectric_point": 9.230534934997557 }, "id": "OAIKOHHJFB_1651", "locus": "GCA000209735_01649" }, { "type": "cds", "sequence": "contig_1", "start": 923787, "stop": 923972, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C18E", "UniRef:UniRef100_C4XG24", "UniRef:UniRef50_C4XG24", "UniRef:UniRef90_C4XG24" ], "nt": "GTGATTTTAAGTGGTATTCTTTACCGCTATTTTTTTACAATTTATTATAAAATTTGCAAAGATAAAAAAGAAATAAAAATTATGAAATATCCAAAGTCTTTTGTTGATTTTAGTAATATTGATGTTGATTTAGAAGCTAGTAATGAACTCTTTTTAATGGGTCCACTTGTTTTTTTATTTTATTAA", "aa": "MILSGILYRYFFTIYYKICKDKKEIKIMKYPKSFVDFSNIDVDLEASNELFLMGPLVFLFY", "aa_hexdigest": "d7bfa93e70bb7dd942a257ab1b248e14", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C18E", "uniref100_id": "UniRef100_C4XG24", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C18E", "UniRef:UniRef100_C4XG24" ] }, "ips": { "uniref100_id": "UniRef100_C4XG24", "uniref90_id": "UniRef90_C4XG24" }, "psc": { "uniref90_id": "UniRef90_C4XG24", "uniref50_id": "UniRef50_C4XG24" }, "pscc": { "uniref50_id": "UniRef50_C4XG24", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG24" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7363.714, "isoelectric_point": 7.796629142761231 }, "id": "OAIKOHHJFB_1652", "locus": "GCA000209735_01650" }, { "type": "cds", "sequence": "contig_1", "start": 924543, "stop": 925133, "strand": "-", "frame": 3, "gene": "ysdC", "product": "Aminopeptidase ysdC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P1S5" ], "nt": "ATGTCAGGTGAAGCAATTGATATGCCTAATAATTTAATTGCTGGTAAAGCAATGGATAATAGAGCTGGTGTTACAGTTATTGACTTTTTAGCAAACAATATTAAAGATTTAGATTTACCGAATGAAACATATATTGTTGGTACAGTACAAGAAGAAATTGGAACAAGAGGTGCTAAAACTTCAGTTTCAATTATTGAACCAGATGTTGCTTTTGCAATTGATACAGGAGCAAGTCATGATACGACAGGATGTATTAAAGGAACTCCAGTTTTAGGTAAAGGTGTAGCTCTTTTAATACAAGATGGTGCTGTTATTACAGATCCTAAATTAGTTAACATGTTAGTAAAAATTGCTGAAAAACACAAAATTCCAGCTTATAAATATATAGCCGAAGGTGGCGGTACTGATGGAGCTAGTTTACAATATGGTAAAGGTGGGGTTCCTACAATTACTCTAAGTTTACCTCAACGTTATTTACACTCACCAATAGGCGTTTGTTCATTAGTAGATATTCAAGCAACAATTGATTTAATTACAGAATTTGTCAAAGCTTTTGACAACAAAATGTTAAATGAATTAAAAGCTAAATAA", "aa": "MSGEAIDMPNNLIAGKAMDNRAGVTVIDFLANNIKDLDLPNETYIVGTVQEEIGTRGAKTSVSIIEPDVAFAIDTGASHDTTGCIKGTPVLGKGVALLIQDGAVITDPKLVNMLVKIAEKHKIPAYKYIAEGGGTDGASLQYGKGGVPTITLSLPQRYLHSPIGVCSLVDIQATIDLITEFVKAFDNKMLNELKAK", "aa_hexdigest": "aa14b09513a8db6338a005c2107b1327", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_UPI002FE1AB9A", "query_cov": 1.0051020408163265, "subject_cov": 0.933649289099526, "identity": 0.5529999999999999, "score": 225.0, "evalue": 5.93e-72, "valid": false, "product": "M42 glutamyl aminopeptidase", "uniref50_id": "UniRef50_A0A3B0P1S5" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P1S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P1S5" ], "product": "Aminopeptidase ysdC" }, "genes": [ "ysdC" ], "id": "OAIKOHHJFB_1653", "locus": "GCA000209735_01651" }, { "type": "cds", "sequence": "contig_1", "start": 925161, "stop": 925313, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTAACAAATAGAGACAATAAAGAAATTTTTGGTATTTTTGGTCATACATCAATTCATATTATGGAAGCTGAAAAACGTGCTAAAGTTCCTACAACAAAAGAAATGTTTGTTGACTGCGGATTCAATTCAAAAGAAGAAGCTGAAAAATGA", "aa": "MVTNRDNKEIFGIFGHTSIHIMEAEKRAKVPTTKEMFVDCGFNSKEEAEK", "aa_hexdigest": "f7e0d5ae347e5fe47023b9314bbd5913", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5745.502199999999, "isoelectric_point": 6.056331062316894 }, "id": "OAIKOHHJFB_1654", "locus": "GCA000209735_01652" }, { "type": "cds", "sequence": "contig_1", "start": 925344, "stop": 925628, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAATATGATAAATTAGCAAAAAGATTAAAAGAATACATGGCTATTGAAGCTATGAGTAGATATGAAGAACCTGTTGTTGAAGCTTTAAAGAAGAATACAGCTTCAAAAAATTTTGAATATTCAAGAGATGGTTTTGGTTCTTTAATTATTAAAAATAAAACAAAAGTTAAAAATGCACCAAAAATTATGATTGCTGCACACATGGATGAAGTTGGTTATTTAGTTAGATCAATCGAACCAAATGGTAACTTATTAGTTTCAGTTGTTGGAGGTGTTTGA", "aa": "MAKYDKLAKRLKEYMAIEAMSRYEEPVVEALKKNTASKNFEYSRDGFGSLIIKNKTKVKNAPKIMIAAHMDEVGYLVRSIEPNGNLLVSVVGGV", "aa_hexdigest": "d833bab3ea9363b9634ee1fce8aed2cc", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10508.202299999999, "isoelectric_point": 9.486925697326662 }, "id": "OAIKOHHJFB_1655", "locus": "GCA000209735_01653" }, { "type": "cds", "sequence": "contig_1", "start": 926414, "stop": 926632, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00690.31" ], "nt": "ATGAAAAAAACTGTTAAAAATAAGAAAAACAATGAAGCTTTAATAATAAAAAATAGATTAGTTGAAGCTGCAAATATGAATCAAAATGAAATTTATGAAAAATATAATTCTTCAAATTTAGGCATTACAAAAAATGAAATAATAGAAGAAAACAAAGAAAATTATGGAGTTAATAAACTCAGCAAAAAGAAAAGAGGCGGAGTATTAAAAAGAATATGA", "aa": "MKKTVKNKKNNEALIIKNRLVEAANMNQNEIYEKYNSSNLGITKNEIIEENKENYGVNKLSKKKRGGVLKRI", "aa_hexdigest": "f4d0319613161825ce66d2a0340f6dd1", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00690.31", "name": "Cation_ATPase_N", "length": 48, "aa_cov": 0.6527777777777778, "hmm_cov": 0.9791666666666666, "evalue": 6.907085008381228e-07, "score": 24.98471450805664, "start": 25, "stop": 71 } ], "seq_stats": { "molecular_weight": 8336.564999999995, "isoelectric_point": 9.946262931823732 }, "id": "OAIKOHHJFB_1656", "locus": "GCA000209735_01654" }, { "type": "cds", "sequence": "contig_1", "start": 926575, "stop": 926751, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACACAGTTCAAGGTTCGGATTTTTCACCTTTAGTTAAAGGAAGTATTATGTCAGTAATTAATGAAACTATTGAAAGTAAAATCAAGATCAATGAAAAAGGATTGAAGAAGCTATCTCATATTCTTTTTAATACTCCGCCTCTTTTCTTTTTGCTGAGTTTATTAACTCCATAA", "aa": "MDTVQGSDFSPLVKGSIMSVINETIESKIKINEKGLKKLSHILFNTPPLFFLLSLLTP", "aa_hexdigest": "903eb4c9a151afbd9cd8e2011158b000", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6431.557799999997, "isoelectric_point": 8.121356391906737 }, "id": "OAIKOHHJFB_1657", "locus": "GCA000209735_01655" }, { "type": "cds", "sequence": "contig_1", "start": 926762, "stop": 927235, "strand": "+", "frame": 2, "gene": null, "product": "HAD-IC family P-type ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002AC587A1" ], "nt": "ATGGTTATAATTAGTGGAATCTTACACTTTGTTGAAGAAACGCGGAGTAGTGCTAGTGCTGAAAAATTAGTCAAAATGATTCAAACAACTACTAGAGTAGAACGAAATGGTGTCGCTTATGAAATACCACTTGAAGAAGTAGTTGTAGGCGATATTGTTGTTTTAGCTGCGGGTGATATTATTCCTGCTGATGTTAGAATTCTTAGTGCAACAGACCTTTTTGTTTCACAATCTCAATTGACAGGCGAAAGTGATTCAATTGAAAAGTTTCCTAATTTAGTTCCAAATTATGATTATGATAATGTAACTGATTATCACAACTTAGCTTTTATGGGTTCTAACGTTATTTCAGGCTCAGCTAAAGCTATGGTTGTAGTTACTGGTGATCAAACTTATATTGGTCAAGTAGCTCAAAAAATTAATGAAAAAGCTCCTAAAACCGATTTTGAAAAAGGAATCAAATCAATTAGTTGA", "aa": "MVIISGILHFVEETRSSASAEKLVKMIQTTTRVERNGVAYEIPLEEVVVGDIVVLAAGDIIPADVRILSATDLFVSQSQLTGESDSIEKFPNLVPNYDYDNVTDYHNLAFMGSNVISGSAKAMVVVTGDQTYIGQVAQKINEKAPKTDFEKGIKSIS", "aa_hexdigest": "fb3ae051800ebd2e30134e9a80545abf", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002AC587A1", "query_cov": 1.0063694267515924, "subject_cov": 0.8272251308900523, "identity": 0.551, "score": 156.0, "evalue": 1.46e-45, "valid": false, "product": "HAD-IC family P-type ATPase", "uniref50_id": "UniRef50_UPI002AC587A1" }, "pscc": { "uniref50_id": "UniRef50_UPI002AC587A1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002AC587A1" ], "product": "HAD-IC family P-type ATPase" }, "genes": [], "id": "OAIKOHHJFB_1658", "locus": "GCA000209735_01656" }, { "type": "cds", "sequence": "contig_1", "start": 927251, "stop": 928639, "strand": "+", "frame": 2, "gene": "mgtA", "product": "magnesium-translocating P-type ATPase", "db_xrefs": [ "EC:7.2.2.14", "GO:0015444", "GO:0015693", "GO:0016020", "KEGG:K01531", "SO:0001217", "UniRef:UniRef50_A0A502M658", "UniRef:UniRef90_C4XG28" ], "nt": "ATGTTAGTAGCTGTACCTCTTGTTTTAATAATCACAGGAACAAGAGCTTCAATTAAAGGAGAACATGAAAAATGGGTTGATGCTTTATTATTTGCAATTTCAGTTGCAATTGGCTTAACTCCTGAAATGTTGCCGATGATAATTACAAGTACTCTAGCTAAAGGTGCTATGAGTATGTCTAAAAAACAAACAATTGTTAAAAGTTTAAACTCAATTCAAAACTTTGGTGCTATGGATATCTTCTGTACAGATAAAACAGGTACCTTAACCTTAGATCAAGTTGTGCTCGAAAGACACTTGGATGTAAGAGGCTTAGAAAATATTAAAGTTTTAAAATATGGATTCTTGAATAGTTATTATCAAACAGGTCTTAAAAACCTTTTAGATATATCTATTATTAATAAGACAGATGAATTAAGTGATGTTGAAATGGAACTAAGATCACTTGAAGAAATCTATAAAAAAGTTGATGAAATACCTTTTGACTTTATTCGTAAAAGAATGTCAGTCGTGGTTAAAAGCATTAAAAGCGATAAAACACAACTTATAACTAAAGGTGCAGTTGAAGAAATTTTATCAATTTGCTCAAAATTAGAATACAAAGGTGAAGTAGTCGACTTAGATGAAAAAATGATTCAAAAAGTTTTAAAACAAGTTGACAAACTAAATGATGAAGGTATGCGGGTAATAGCAATGGCTCGTAAAAGTAACCCTAGTCAAGTTGGTAAATTTGGTGTAGCAGATGAAAAGGATATGATTCTAATTGGCTATCTAGCTTTCTTAGATCCACCAAAAGAATCAACTAAAAGTGCAGTTGAAAACCTTCACAACTTAGGTGTCGAAGTAAAAATCTTAACTGGTGATAATGCCAGAGTTACTAGAGCAATTTGTGCTCAAGTTGGAATTCCTACAGATAAAATTATGCTTGGTAAAGACTTAATGGAATTAAGCGATGAAGAATTGCAAAAAGTTGCTAATGAATACAATATTTATGCTAAATTAAGTCCTGACCAAAAAGCTCGTGTAATAACAGCACTTAGAGCTAATGGCCATGTTGTAGGTTATATGGGTGATGGAATTAATGATGCTCCTGCTATGAAAGTCGCTGATGTTTCTATTTCAGTTGATACTGCAGTCGATATTGCTAAAGAAAGTGCAAACATTATTCTTTTAGAAAAAGACTTAAATGTTTTAGCAACTGGAATTACTGAAGGCCGCAAGACATATGCTAATATGAATAAATATGTCAAAATGACAGTAAGTAGTAACTTTGGAAATATCATTAGCATGATATTAGCTTCGATTCTTATTCCTTTTGTGCCTTTAATGGCAATTCAAGTTTTATTTTTAAACTTAATTTATGATTTTGCTTGTGGAACAATTCCTTGA", "aa": "MLVAVPLVLIITGTRASIKGEHEKWVDALLFAISVAIGLTPEMLPMIITSTLAKGAMSMSKKQTIVKSLNSIQNFGAMDIFCTDKTGTLTLDQVVLERHLDVRGLENIKVLKYGFLNSYYQTGLKNLLDISIINKTDELSDVEMELRSLEEIYKKVDEIPFDFIRKRMSVVVKSIKSDKTQLITKGAVEEILSICSKLEYKGEVVDLDEKMIQKVLKQVDKLNDEGMRVIAMARKSNPSQVGKFGVADEKDMILIGYLAFLDPPKESTKSAVENLHNLGVEVKILTGDNARVTRAICAQVGIPTDKIMLGKDLMELSDEELQKVANEYNIYAKLSPDQKARVITALRANGHVVGYMGDGINDAPAMKVADVSISVDTAVDIAKESANIILLEKDLNVLATGITEGRKTYANMNKYVKMTVSSNFGNIISMILASILIPFVPLMAIQVLFLNLIYDFACGTIP", "aa_hexdigest": "6480c29640fac73003734c8234ee5734", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0009C0D368", "query_cov": 0.9523809523809523, "subject_cov": 0.9128630705394191, "identity": 0.525, "score": 447.0, "evalue": 4.33e-151, "valid": false, "gene": "mgtA", "product": "magnesium-translocating P-type ATPase", "ec_ids": [ "7.2.2.14" ], "uniref50_id": "UniRef50_A0A2X2TA75", "kegg_orthology_id": "K01531", "go_ids": [ "GO:0015444", "GO:0015693", "GO:0016020" ] }, "genes": [ "mgtA" ], "truncated": "both", "pseudogene": { "start": 926951, "stop": 928942, "observations": { "mutation": [ 927233, 928637, 928679, 928832, 928841, 928859 ], "stop": [ 927233, 928637, 928679, 928832, 928841, 928859 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG28", "query_cov": 1.0, "subject_cov": 0.5032679738562091, "identity": 1.0, "score": 868.0, "evalue": 1.57e-310, "gene_start": 1, "gene_end": 462, "reference_start": 294, "reference_end": 755, "reference_sequence": "MINNIYYLNGREVIMKKTVKNKKNNEALIIKNRLVEAANMNQNEIYEKYNSSNLGITKNEIIEENKENYGVNKLSKKKRGGVLKRIWDSFFNPFSLILILLSIVSLITDIILPLTKGEKSEPWTVSIILTMVIISGILHFVEETRSSASAEKLVKMIQTTTRVERNGVAYEIPLEEVVVGDIVVLAAGDIIPADVRILSATDLFVSQSQLTGESDSIEKFPNLVPNYDYDNVTDYHNLAFMGSNVISGSAKAMVVVTGDQTYIGQVAQKINEKAPKTDFEKGIKSISWLLIKIMLVAVPLVLIITGTRASIKGEHEKWVDALLFAISVAIGLTPEMLPMIITSTLAKGAMSMSKKQTIVKSLNSIQNFGAMDIFCTDKTGTLTLDQVVLERHLDVRGLENIKVLKYGFLNSYYQTGLKNLLDISIINKTDELSDVEMELRSLEEIYKKVDEIPFDFIRKRMSVVVKSIKSDKTQLITKGAVEEILSICSKLEYKGEVVDLDEKMIQKVLKQVDKLNDEGMRVIAMARKSNPSQVGKFGVADEKDMILIGYLAFLDPPKESTKSAVENLHNLGVEVKILTGDNARVTRAICAQVGIPTDKIMLGKDLMELSDEELQKVANEYNIYAKLSPDQKARVITALRANGHVVGYMGDGINDAPAMKVADVSISVDTAVDIAKESANIILLEKDLNVLATGITEGRKTYANMNKYVKMTVSSNFGNIISMILASILIPFVPLMAIQVLFLNLIYDFACGTIPWDKVDKELITKPRKWNAKSILRFMLWFGPVSSIVDILSFILLYYVFIPYAHPSVAKDSEQFKMLFWTGWFIISMWTQSLVIHFIRTEKAAFIQSKPAFILLFFSIFGSVLITITPYIPGLNTALKVTQLNPWFYGFLVGSIALYISLVLIVKKIYIIKYNELL" }, "paralog": false, "identity": 0.9909502262443439, "score": 1252.0, "evalue": 0.0, "description": "Internal stop codon at 927233, 928637, 928679, 928832, 928841, 928859. Nonsense mutation around 927233, 928637, 928679, 928832, 928841, 928859.", "psc": { "uniref90_id": "UniRef90_C4XG28", "gene": "mgtA", "product": "magnesium-translocating P-type ATPase", "ec_ids": [ "7.2.2.14" ], "uniref50_id": "UniRef50_A0A502M658", "kegg_orthology_id": "K01531", "go_ids": [ "GO:0015444", "GO:0015693", "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG28", "EC:7.2.2.14", "UniRef:UniRef50_A0A502M658", "KEGG:K01531", "GO:0015444", "GO:0015693", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_A0A502M658", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A502M658" ], "product": "Magnesium-transporting ATPase, P-type 1" } }, "id": "OAIKOHHJFB_1659", "locus": "GCA000209735_01657" }, { "type": "cds", "sequence": "contig_1", "start": 930081, "stop": 930398, "strand": "-", "frame": 3, "gene": null, "product": "YgjP-like metallopeptidase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG30", "UniRef:UniRef90_C4XG30" ], "nt": "ATGACTCAAAGAAAAAATAATTTAGTTTTAATTAAACACATCATAATTGATGATGTTAGTTATCCAGTTTATTCAAAAGTTAATGTCCGTTGTGAAAATATAATCGTTAGATTTAATAGTGACCAAAAAGTATTTGAGGTCACTAGTTTTAGACATATTAGTGAGGCTGATTATAATTTTCATAATTTAGATAGACTACTTATTAAAGCTTTTAAAAAAGCAAATAAAAGATTAAATAATGCTATAGTTTTAAATTTAACTAGCGGAGTTGAAGAACACTACATTAGTTTTTTTGGCAAAGAAACATTATTAAAATGA", "aa": "MTQRKNNLVLIKHIIIDDVSYPVYSKVNVRCENIIVRFNSDQKVFEVTSFRHISEADYNFHNLDRLLIKAFKKANKRLNNAIVLNLTSGVEEHYISFFGKETLLK", "aa_hexdigest": "ff7b35607f9d980cca6abd0657a87b27", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 929778, "stop": 930398, "observations": { "mutation": [ 929852, 930083 ], "stop": [ 929852, 930083 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG30", "query_cov": 1.0, "subject_cov": 0.41015625, "identity": 1.0, "score": 207.0, "evalue": 9.16e-66, "gene_start": 1, "gene_end": 105, "reference_start": 1, "reference_end": 105, "reference_sequence": "MTQRKNNLVLIKHIIIDDVSYPVYSKVNVRCENIIVRFNSDQKVFEVTSFRHISEADYNFHNLDRLLIKAFKKANKRLNNAIVLNLTSGVEEHYISFFGKETLLKWSFHYRDTSDGVYDKENNILTIRCSIKCASDQNKRLKVFEKTLTKFLEDYISKKQLVYSALMQINNPYFYVRMKKSAWGTHHKKYNVFSKLSYSLFLVALDPKYIDYVIVHELCHYFHMDHSEEFYKLGEFYYPNFKEYSDTINKHKINFW" }, "paralog": false, "identity": 0.9902912621359223, "score": 405.0, "evalue": 2.73e-146, "description": "Internal stop codon at 929852, 930083. Nonsense mutation around 929852, 930083.", "psc": { "uniref90_id": "UniRef90_C4XG30", "product": "YgjP-like metallopeptidase domain-containing protein", "uniref50_id": "UniRef50_C4XG30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG30", "UniRef:UniRef50_C4XG30" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG30" ], "product": "YgjP-like metallopeptidase domain-containing protein" } }, "id": "OAIKOHHJFB_1660", "locus": "GCA000209735_01658" }, { "type": "cds", "sequence": "contig_1", "start": 930379, "stop": 930516, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGAAGATCGAAACTTAAATAAAGATGAATTAATGTTAATCAATGAATCAGTGAGTTCAAATAGAAGTGCTTCACTTAAAGACATTAAACGTTTTGGAAAGATCTTAAACAAAAATGACTCAAAGAAAAAATAA", "aa": "MSEDRNLNKDELMLINESVSSNRSASLKDIKRFGKILNKNDSKKK", "aa_hexdigest": "8e005d109165793d4f611c438c2825e1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5195.8859, "isoelectric_point": 9.77445468902588 }, "id": "OAIKOHHJFB_1661", "locus": "GCA000209735_01659" }, { "type": "cds", "sequence": "contig_1", "start": 930574, "stop": 931080, "strand": "-", "frame": 2, "gene": null, "product": "NERD domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG31", "UniRef:UniRef90_C4XG31" ], "nt": "ATGATTAAACCAAGTAGAGAAATGATAATTGGACTATCAGTTGGAATTCCTTTAACACTATTAATTATTGTGCTAGTGTTAACTTTTTTAATTATCAAACTTATTAAAAGCAAACGCAATAATTCAATTGGTTTTCGTTTTGAAGCAAAAATTAGAAATAAACTTCTAACTATTGCTAAAGATAAAAACTACAAATACTTAAATGGTGGTTTATTTAAATATGGACAAAGTCAAATATTTGAACTTGATGGTATTTTAATTAGTAATAAGGCAGTTTTTATTATTGAAGTTAAATATTATGTTGGCCATCTTAGTGGTGAAGCTACTAATGCTGAATTAGAGTTAAGAAGCAAAAGCAATAAAGTTTTGAAATTTAAAAATCCGCTTATTCAAAACTTTAAACACATTCAACACTTTTACAAAATGTGTGGCTTTAACTTCCCAGTATTTTCATTGGTAATCTTGCCTTCAAATACTACTTTTGATATAGCTAACTTAGAATCATGA", "aa": "MIKPSREMIIGLSVGIPLTLLIIVLVLTFLIIKLIKSKRNNSIGFRFEAKIRNKLLTIAKDKNYKYLNGGLFKYGQSQIFELDGILISNKAVFIIEVKYYVGHLSGEATNAELELRSKSNKVLKFKNPLIQNFKHIQHFYKMCGFNFPVFSLVILPSNTTFDIANLES", "aa_hexdigest": "b7fec0ca1287f650dd67e23f100062f5", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 930379, "stop": 931113, "observations": { "mutation": [ 930576 ], "stop": [ 930576 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG31", "query_cov": 1.0, "subject_cov": 0.6885245901639344, "identity": 1.0, "score": 319.0, "evalue": 4.55e-109, "gene_start": 1, "gene_end": 168, "reference_start": 12, "reference_end": 179, "reference_sequence": "MQILILNIIINMIKPSREMIIGLSVGIPLTLLIIVLVLTFLIIKLIKSKRNNSIGFRFEAKIRNKLLTIAKDKNYKYLNGGLFKYGQSQIFELDGILISNKAVFIIEVKYYVGHLSGEATNAELELRSKSNKVLKFKNPLIQNFKHIQHFYKMCGFNFPVFSLVILPSNTTFDIANLESWALVTTEDQVANLLEDVAKDMSEDRNLNKDELMLINESVSSNRSASLKDIKRFGKILNKNDSKKK" }, "paralog": false, "identity": 0.9918032786885246, "score": 465.0, "evalue": 5.06e-169, "description": "Internal stop codon at 930576. Nonsense mutation around 930576.", "psc": { "uniref90_id": "UniRef90_C4XG31", "product": "NERD domain-containing protein", "uniref50_id": "UniRef50_C4XG31", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG31", "UniRef:UniRef50_C4XG31" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG31", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG31" ], "product": "NERD domain-containing protein" } }, "id": "OAIKOHHJFB_1662", "locus": "GCA000209735_01660" }, { "type": "cds", "sequence": "contig_1", "start": 931159, "stop": 931347, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGCAAAGAAAATTATCTCAATTTTAGGTTCAGTAAATAAGAATAGTTTAAGTAATAAAATCAACAATATAGTAAGCAGTAAATTGTTTAAAAAATATCCTAATAGTGAAATCACTTTATTAGATTTAGCAAATTCGCCTTTTAGTGAATTAATGCTTAATGGTCATGACACAACAGCTTTTTGA", "aa": "MSAKKIISILGSVNKNSLSNKINNIVSSKLFKKYPNSEITLLDLANSPFSELMLNGHDTTAF", "aa_hexdigest": "6b19def8555b70166c7db5f108cdfd31", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6798.7907, "isoelectric_point": 9.523801612854005 }, "id": "OAIKOHHJFB_1663", "locus": "GCA000209735_01661" }, { "type": "cds", "sequence": "contig_1", "start": 931417, "stop": 931581, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02525.22" ], "nt": "ATGATTAATTTTAATTACAGTGCTTTAGTTAAAAACTTCATTGATGCAATTTGTATTGCTGATCATTCATTTAGTTATAAATATAGTAAAAAGAATGGATCAGTTGGTTTATTAAACAAAATGAAAGTTATTATTATTGGTACTCAAGGAGCTCCAAAAGGTTGA", "aa": "MINFNYSALVKNFIDAICIADHSFSYKYSKKNGSVGLLNKMKVIIIGTQGAPKG", "aa_hexdigest": "261b72bc83ebc312a1971da398a55dd2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02525.22", "name": "Flavodoxin_2", "length": 53, "aa_cov": 0.9629629629629629, "hmm_cov": 0.9622641509433962, "evalue": 4.4533778535724374e-10, "score": 35.646446228027344, "start": 1, "stop": 52 } ], "seq_stats": { "molecular_weight": 5909.8963, "isoelectric_point": 9.66846866607666 }, "id": "OAIKOHHJFB_1664", "locus": "GCA000209735_01662" }, { "type": "cds", "sequence": "contig_1", "start": 931606, "stop": 931749, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGAAGGAACATTTAACTTTTTAGGAGCTAAAGAAGTTAAGACTTTATTAGTAGCTGGCACTAAAGTGACACCTCTTAAGGACATGAGTCAAGAAGAAATAATTAAGCAATATCAAAAAGAAATTGATGCTTTAATTTAA", "aa": "MLEGTFNFLGAKEVKTLLVAGTKVTPLKDMSQEEIIKQYQKEIDALI", "aa_hexdigest": "553991972b2c020f8bed4c015ac62b2c", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5283.1615999999985, "isoelectric_point": 5.144690513610839 }, "id": "OAIKOHHJFB_1665", "locus": "GCA000209735_01663" }, { "type": "cds", "sequence": "contig_1", "start": 931826, "stop": 931984, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01029.23" ], "nt": "ATGTCAATTGAACCTAAAATAATAATTAATGAATCAGTTGAAATAACTAAATATTATTTTGAAGATGAAAAAAACTTTTACAAAATGGTTAATGCAATTCTAGAAAAAGTTTATAAATTCTTTGTTATCAATGAAGTTATTTTAAAAAAACAAGATTAA", "aa": "MSIEPKIIINESVEITKYYFEDEKNFYKMVNAILEKVYKFFVINEVILKKQD", "aa_hexdigest": "dc21ac322b43ef772389b9ca666207f5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01029.23", "name": "NusB", "length": 38, "aa_cov": 0.7307692307692307, "hmm_cov": 0.9473684210526315, "evalue": 1.708300842107331e-07, "score": 27.785888671875, "start": 2, "stop": 39 } ], "seq_stats": { "molecular_weight": 6336.368700000002, "isoelectric_point": 5.3466394424438475 }, "id": "OAIKOHHJFB_1666", "locus": "GCA000209735_01664" }, { "type": "cds", "sequence": "contig_1", "start": 932045, "stop": 932245, "strand": "-", "frame": 1, "gene": null, "product": "NusB/RsmB/TIM44 domain-containing protein", "db_xrefs": [ "KEGG:K03625", "SO:0001217", "UniRef:UniRef50_C4XG33", "UniRef:UniRef90_C4XG33" ], "nt": "ATGCAAAACAGACGTCAATTTCGTTTAGGTATAATTAATGTTCTTTACCGTTATGAATTAATGGATCAAAAAATCAATGTCAATGAATTATTTGATTCAGAAAGTAACTTAAGTAATGAACAATTTTCGCATCTTGAAAAGATAGCAAAAAACTATGATTTTTATAAAAAAGCAGTCAGTCAATTTTTAAAAGAAGGTTGA", "aa": "MQNRRQFRLGIINVLYRYELMDQKINVNELFDSESNLSNEQFSHLEKIAKNYDFYKKAVSQFLKEG", "aa_hexdigest": "7d736dd278c923cfe77172f929eee637", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 931826, "stop": 932245, "observations": { "mutation": [ 932041, 932047 ], "stop": [ 932041, 932047 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG33", "query_cov": 1.0, "subject_cov": 0.4748201438848921, "identity": 1.0, "score": 129.0, "evalue": 2.5e-37, "gene_start": 1, "gene_end": 66, "reference_start": 1, "reference_end": 66, "reference_sequence": "MQNRRQFRLGIINVLYRYELMDQKINVNELFDSESNLSNEQFSHLEKIAKNYDFYKKAVSQFLKEGWSWERISPLIRAILINGAHELMSIEPKIIINESVEITKYYFEDEKNFYKMVNAILEKVYKFFVINEVILKKQD" }, "paralog": false, "identity": 0.9856115107913669, "score": 267.0, "evalue": 6.11e-94, "description": "Internal stop codon at 932041, 932047. Nonsense mutation around 932041, 932047.", "psc": { "uniref90_id": "UniRef90_C4XG33", "product": "NusB/RsmB/TIM44 domain-containing protein", "uniref50_id": "UniRef50_C4XG33", "kegg_orthology_id": "K03625", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG33", "UniRef:UniRef50_C4XG33", "KEGG:K03625" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG33", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG33" ], "product": "NusB/RsmB/TIM44 domain-containing protein" } }, "id": "OAIKOHHJFB_1667", "locus": "GCA000209735_01665" }, { "type": "cds", "sequence": "contig_1", "start": 932319, "stop": 932729, "strand": "-", "frame": 3, "gene": null, "product": "DUF1934 domain-containing protein", "db_xrefs": [ "RefSeq:WP_013354794.1", "SO:0001217", "UniParc:UPI0001A8C198", "UniRef:UniRef100_C4XG34", "UniRef:UniRef50_C4XG34", "UniRef:UniRef90_C4XG34" ], "nt": "ATGAAAATAAGATTTACTTCAATTCAAGATCATAAAATTAAAAACCAAGAACCTAAAATAGTTTCTTTTGAAGCTGATGCAGAATATGATTTATTTTTAGATGATGAAGATCCAAAGCAATTACAATATCATACATATCATTTTAAAGAGCCTTCAATTGGTGAATCTAACCGTATAGAAATCAATAGTGAAAGAGTAAATATTTTTACAGGAATGTCAACGTTGACAATGTTAAAAGGTCAAAAAGCTCCTAACTCTTTTGTTAAAGTAGATGGCACACAATTCTTTTTAAAACCATTTTTACATGAAGTTGTTATTGAAGGCAATATTAAAAAATTCAAATATGAATTATTTGGCCCTAAAGATGATTTAATTGGTGAATTCAATGTTTCGATTGAAGAATTAGAATAA", "aa": "MKIRFTSIQDHKIKNQEPKIVSFEADAEYDLFLDDEDPKQLQYHTYHFKEPSIGESNRIEINSERVNIFTGMSTLTMLKGQKAPNSFVKVDGTQFFLKPFLHEVVIEGNIKKFKYELFGPKDDLIGEFNVSIEELE", "aa_hexdigest": "2174371c5bb31c858753072833bd3158", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001A8C198", "ncbi_nrp_id": "WP_013354794.1", "uniref100_id": "UniRef100_C4XG34", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C198", "RefSeq:WP_013354794.1", "UniRef:UniRef100_C4XG34" ] }, "ips": { "uniref100_id": "UniRef100_C4XG34", "uniref90_id": "UniRef90_C4XG34" }, "psc": { "uniref90_id": "UniRef90_C4XG34", "product": "DUF1934 domain-containing protein", "uniref50_id": "UniRef50_C4XG34" }, "pscc": { "uniref50_id": "UniRef50_C4XG34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG34" ], "product": "DUF1934 domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_1668", "locus": "GCA000209735_01666" }, { "type": "cds", "sequence": "contig_1", "start": 932726, "stop": 933004, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF19269.4" ], "nt": "ATGCAGCCATATTTAAAACCAACAAATAAAAAACCAGTTGTCGAAGCTGAAAATCATGATGTAATTTTAACTTTTAAAGATTTATTAAATAAAAACACTTTTACTATTGAAAATATTCAAGCTGCAATTGACCAAACAAAAGAAATTACAGGTAAAAAAGGCAAAAATCTATTTATGCCAATACGTCTAGCAACCACTTACATTGAACATGGTCCTGAATTAGCTAAAGCAATTTATTTATATGGCGAAGACTTAATTAAAGAAAGGTTAGCAAAATGA", "aa": "MQPYLKPTNKKPVVEAENHDVILTFKDLLNKNTFTIENIQAAIDQTKEITGKKGKNLFMPIRLATTYIEHGPELAKAIYLYGEDLIKERLAK", "aa_hexdigest": "716b3e44b08ce9adaafe89facb90e8e2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF19269.4", "name": "Anticodon_2", "length": 79, "aa_cov": 0.8478260869565217, "hmm_cov": 1.0, "evalue": 5.019436854541526e-10, "score": 36.024383544921875, "start": 15, "stop": 92 } ], "seq_stats": { "molecular_weight": 10529.14899999999, "isoelectric_point": 8.831411170959473 }, "id": "OAIKOHHJFB_1669", "locus": "GCA000209735_01667" }, { "type": "cds", "sequence": "contig_1", "start": 933254, "stop": 933370, "strand": "-", "frame": 1, "gene": null, "product": "glutamate--tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PLI0", "UniRef:UniRef90_UPI0021FF3334" ], "nt": "ATGGAAGGTAAAAAACTTTCAAAACGTGATACTAGTTTAAAACAATTTATTGAAGATTATCGTAATGAAGGCTACCATCCTGAAGGTGTATTTAACTTTTTAAGTTTGCTTGGTTGA", "aa": "MEGKKLSKRDTSLKQFIEDYRNEGYHPEGVFNFLSLLG", "aa_hexdigest": "0fa7d902c7d175366f672234bafc8822", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 933206, "stop": 933370, "observations": { "mutation": [ 933256 ], "stop": [ 933256 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0021FF3334", "query_cov": 1.0, "subject_cov": 0.6909090909090909, "identity": 0.868, "score": 73.6, "evalue": 1.28e-16, "gene_start": 1, "gene_end": 38, "reference_start": 1, "reference_end": 38, "reference_sequence": "MEGKKLSKRDTSLKQFIEDYKNEGYLPEAIFNFLTLLGWTASDASELMSKDEIIS" }, "paralog": false, "identity": 0.7407407407407407, "score": 83.2, "evalue": 2.88e-23, "description": "Internal stop codon at 933256. Nonsense mutation around 933256.", "psc": { "uniref90_id": "UniRef90_UPI0021FF3334", "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_A0A3B0PLI0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0021FF3334", "UniRef:UniRef50_A0A3B0PLI0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PLI0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PLI0" ], "product": "Glutamate--tRNA ligase" } }, "id": "OAIKOHHJFB_1670", "locus": "GCA000209735_01668" }, { "type": "cds", "sequence": "contig_1", "start": 933920, "stop": 934111, "strand": "-", "frame": 1, "gene": null, "product": "Glutamate--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2N2SDT7" ], "nt": "ATGAAAAAAATAAGAACCAGATATGCGCCAAGTCCAACAGGTTACTTACACATTGGAGGTGCTAGAACAGCATTATTTTGCTATTTATATGCTAAGCACTTTAAAGGTGATTTTGTTTTCAGATTAGAAGACACTGATGTTAAAAGAAATGTAGCTGATGGTGAAAGAAGTCAATTAGAAAACTTAGCTTGA", "aa": "MKKIRTRYAPSPTGYLHIGGARTALFCYLYAKHFKGDFVFRLEDTDVKRNVADGERSQLENLA", "aa_hexdigest": "71b6c1c2e80d80f5a82ea70deef88add", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0037F98A17", "query_cov": 0.9523809523809523, "subject_cov": 0.8571428571428571, "identity": 0.5670000000000001, "score": 79.3, "evalue": 2.79e-18, "valid": false, "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_A0A2N2SDT7" }, "pscc": { "uniref50_id": "UniRef50_A0A2N2SDT7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2N2SDT7" ], "product": "Glutamate--tRNA ligase" }, "genes": [], "id": "OAIKOHHJFB_1671", "locus": "GCA000209735_01669" }, { "type": "cds", "sequence": "contig_1", "start": 934398, "stop": 935267, "strand": "-", "frame": 3, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "RefSeq:WP_013526998.1", "SO:0001217", "UniParc:UPI0001F59BF5", "UniRef:UniRef100_C4XG36", "UniRef:UniRef50_C4XG36", "UniRef:UniRef90_C4XG36" ], "nt": "ATGAAATTAAAAAAAATATTTAAATTTGGAGCATTGTTAACTTCACCAATCATGTTAGTTCCTATTGTTTCATGTGGCAAAAATCAAAAAGATGTTAATTCAACAAAATTCTTTGAATTTGAAAGTCCGATGCCTAAAAACAATAAACCAAATAATGAAATAAGTGAAAAAGAAAAATTTCAAAATTGTCAAAACGCATATGAAAAAATTATCCAATTAGCATCATTTAAAAATGCGCTTCAAAGTGATTTTGATGCAAATAACGTAAGTGAATATGGACCTGAAAAAATTTTAGATTTTAAAGCTAAGGACGGTCTTGCATTACTTAAAGCTACATATTTTAATAAAGAATCTGAATATGAAAAAATAGTTGAAGTTTATAAATCAATTAATCCTACAGGAAACTTTAATGATTATTTAACTAATGAATATAAAAAGATGACAAGTGCAATTAATATTTATCTTAAAGCTGAAGATAATCAAAGTGCAAAAAATATTAAAGAAGAATTAAATGATCCTAAAAAAGTTAAAGAAATAAATAACAAGGTTAATAATATAAAAGAAACAATTAACAAATTGATTGATAAATTAAATGAAAATCCTGAAATCAAAAGTAAAGTTGATGAATCTTTGAAAAAACAAAACTTATCAATAGAAAAAATTAAAGAAAAAATCAGTGGTTATGATAATAATGAAATTGCAAAAGGAATTTTTCTTGGACTTAATGGAATAATAAAGAATAAAGACGAACTATTTAAAAATCCTAATTTATCAAAATTTGCTCCGATTATTCCAAACAGACCTTTTGATAATTTGAATGAATCATTAGATGTAATTAGCAATGTTTTGTCGACTGCAAATCTATTTTAA", "aa": "MKLKKIFKFGALLTSPIMLVPIVSCGKNQKDVNSTKFFEFESPMPKNNKPNNEISEKEKFQNCQNAYEKIIQLASFKNALQSDFDANNVSEYGPEKILDFKAKDGLALLKATYFNKESEYEKIVEVYKSINPTGNFNDYLTNEYKKMTSAINIYLKAEDNQSAKNIKEELNDPKKVKEINNKVNNIKETINKLIDKLNENPEIKSKVDESLKKQNLSIEKIKEKISGYDNNEIAKGIFLGLNGIIKNKDELFKNPNLSKFAPIIPNRPFDNLNESLDVISNVLSTANLF", "aa_hexdigest": "a2d0df164650869171eab2137048e069", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI0001F59BF5", "ncbi_nrp_id": "WP_013526998.1", "uniref100_id": "UniRef100_C4XG36", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59BF5", "RefSeq:WP_013526998.1", "UniRef:UniRef100_C4XG36" ] }, "ips": { "uniref100_id": "UniRef100_C4XG36", "uniref90_id": "UniRef90_C4XG36" }, "psc": { "uniref90_id": "UniRef90_C4XG36", "product": "Lipoprotein", "uniref50_id": "UniRef50_C4XG36" }, "pscc": { "uniref50_id": "UniRef50_C4XG36", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG36" ], "product": "Lipoprotein" }, "genes": [], "id": "OAIKOHHJFB_1672", "locus": "GCA000209735_01670" }, { "type": "cds", "sequence": "contig_1", "start": 935473, "stop": 935580, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAGAAATATTATTTAAAAGATGATTCTAAATTAAGTTATTTAGATTTAATTATGAATACAGCTAACAATAATGATTATTATGTAGAAATGGTTCAAGCTTGA", "aa": "MLKKYYLKDDSKLSYLDLIMNTANNNDYYVEMVQA", "aa_hexdigest": "5c74b3d0f0f832c9857cf9ed2202a669", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4208.783600000002, "isoelectric_point": 4.777908515930175 }, "id": "OAIKOHHJFB_1673", "locus": "GCA000209735_01671" }, { "type": "cds", "sequence": "contig_1", "start": 935856, "stop": 936017, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG38", "UniRef:UniRef90_C4XG38" ], "nt": "ATGAATGAATTAAGATGTAGAAAAGAAGAAAAACTTTATTATAAATTAATACCTTATATTGATCAAAAATATAAAGAATTTTGCTTGCAAATTTATAAAATTGAAAGTGCAAAATTATTAGGAGTTAGAATTCCAATACTTAAAGATTTAGCTAAAAAATGA", "aa": "MNELRCRKEEKLYYKLIPYIDQKYKEFCLQIYKIESAKLLGVRIPILKDLAKK", "aa_hexdigest": "99f63e159400816f497060608c1bab0a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 935736, "stop": 936017, "observations": { "mutation": [ 935819, 935858 ], "stop": [ 935819, 935858 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG38", "query_cov": 1.0, "subject_cov": 0.5698924731182796, "identity": 1.0, "score": 107.0, "evalue": 3.61e-29, "gene_start": 1, "gene_end": 53, "reference_start": 1, "reference_end": 53, "reference_sequence": "MNELRCRKEEKLYYKLIPYIDQKYKEFCLQIYKIESAKLLGVRIPILKDLAKKWYKEIPTKVSEFMWNPYHYLFEEIYFMVFLLKKLMIIKSK" }, "paralog": false, "identity": 0.978494623655914, "score": 183.0, "evalue": 1.09e-61, "description": "Internal stop codon at 935819, 935858. Nonsense mutation around 935819, 935858.", "psc": { "uniref90_id": "UniRef90_C4XG38", "uniref50_id": "UniRef50_C4XG38", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG38", "UniRef:UniRef50_C4XG38" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG38", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG38" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6478.775, "isoelectric_point": 9.481768226623537 }, "id": "OAIKOHHJFB_1674", "locus": "GCA000209735_01672" }, { "type": "cds", "sequence": "contig_1", "start": 936523, "stop": 936816, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGCTGTTATGATTGGTGGTATGGTTCCTCCTCTTGTAATCGCTATTGCAGCTGACTTATTCCCTCAAAAATTTACTGCTAAAGAAAGAAAAGATTCAAAAGTTAACTACTTAATGGGCATGGCATTTATTACTGAAGGTACAATTCCTTATGCAGCTAGTGATCCCTTGAGAGTTATTGGTTCATCAATTTTTGCAAGTACATTAGCTGGTGGAATGGCAGCTGGATTAGGATGCTCAATTCCTGCTCCTCATGGCGGAATCTTTGTATTCGTTGTTGCACAAGGATGA", "aa": "MAAVMIGGMVPPLVIAIAADLFPQKFTAKERKDSKVNYLMGMAFITEGTIPYAASDPLRVIGSSIFASTLAGGMAAGLGCSIPAPHGGIFVFVVAQG", "aa_hexdigest": "385df7906a33e218c1dbc36b538bcc55", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9901.656399999994, "isoelectric_point": 7.90732135772705 }, "id": "OAIKOHHJFB_1675", "locus": "GCA000209735_01673" }, { "type": "cds", "sequence": "contig_1", "start": 936820, "stop": 937086, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATTTATCCAGTATTTGGTATCTTACTTATTGCTGTTATTATGTTCTTTGTAATTAATACACCACTTTCATATTTAAATTACTACATTCAACAAGGTTTAAACTATGTTGTTAAAAATAACACAATTTCAATTGGCTTTAAAATTGGAATGGGAGATTTACTTGCTGGTATGATGGCAGTTGATATGGGTGGTCCAGTTAACAAAGTTGCATATACTATTGGTGTTGCTTCAGTAGATCCTGATGGAATCAATCAACCATGA", "aa": "MLIYPVFGILLIAVIMFFVINTPLSYLNYYIQQGLNYVVKNNTISIGFKIGMGDLLAGMMAVDMGGPVNKVAYTIGVASVDPDGINQP", "aa_hexdigest": "5a7ff0b3c76abac7bb769a4181b7cc09", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9518.224499999998, "isoelectric_point": 4.576130104064942 }, "id": "OAIKOHHJFB_1676", "locus": "GCA000209735_01674" }, { "type": "cds", "sequence": "contig_1", "start": 937129, "stop": 937737, "strand": "-", "frame": 2, "gene": null, "product": "PTS EIIC type-2 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG39", "UniRef:UniRef90_C4XG39" ], "nt": "TTGACAAATGAAGATATTCAAAAAGCTTATGGTGTCATTGTTGCTGCCGATACAAATATTGAAATCACAAGATTCAATGGTAAAAGATTGCTTCAAACAACTGTAACAAAAGGAATTAAACAACCCAAAGAATTAATACAAGAAATAATAGATGAAAAAGTAGCTGTATATAAATCAGATTCTTCTTCAAATGAAAATACAAATCAAGAAAAATTAAGTTTTGGAAAACAAATATATAAGAATTTAATGAACGGTGTAAGCCATATGCTTCCATTTGTTATTGGTGGCGGTATTCTAATTGCTCTAGCCTTCTTATTTGACTCTTTTTTAGGAAAAGAAGCAGTAGGAAAAGATTTTGGACAATATACTCCATTTTCAAAATTCTTTAAAAATGCTGGTGGATTTGCTTTTGGCTTTATGATGTCAGTGTTTGCTGGTTTTATTGCACATTCAATTGCAGGAAGACCAGGCTTAGCAGTCGGTATGGTAGCTGGAGCCATGGCATCTAGTACACAGTTTGCGGTTTATGAGACTGGTTCTAATGCAGGCTTCTTAGGAGCCTTAATTGGTGGTTTCATTGCCGGATTCTTAGTATTAGGATTAAAATGA", "aa": "MTNEDIQKAYGVIVAADTNIEITRFNGKRLLQTTVTKGIKQPKELIQEIIDEKVAVYKSDSSSNENTNQEKLSFGKQIYKNLMNGVSHMLPFVIGGGILIALAFLFDSFLGKEAVGKDFGQYTPFSKFFKNAGGFAFGFMMSVFAGFIAHSIAGRPGLAVGMVAGAMASSTQFAVYETGSNAGFLGALIGGFIAGFLVLGLK", "aa_hexdigest": "2cd91a8a0e90cade06225c91a85c37f7", "start_type": "TTG", "rbs_motif": "4Base/6BMM", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 936826, "stop": 937737, "observations": { "mutation": [ 937119, 937131 ], "stop": [ 937119, 937131 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG39", "query_cov": 1.0, "subject_cov": 0.43817787418655096, "identity": 0.946, "score": 356.0, "evalue": 8.75e-120, "gene_start": 1, "gene_end": 202, "reference_start": 1, "reference_end": 202, "reference_sequence": "MTNEDIQKAYGVIVAADTNIEITRFNGKRLLQTTVTKGIKQPKELIQEIIDEKVAVYKSDSSSNENTNQEKLSFGKQIYKNLMNGVSHMLPFVIGGGILIALAFLFDSFLGKEAVGKDFGQYTPFSKFFKNAGGFAFGFMMSVFAGFIAHSIAGRPGLAVGMVAGAMASSTQFAVYETGSNAGFLGALIGGFIAGFLVLGLKWCFKWLPKSLDGLKPMLIYPVFGILLIAVIMFFVINTPLSYLNYYIQQGLNYVVKNNTISIGFKIGMGALLAGMMAVDMGGPVNKVAYTIGVASVDPDGINQPWIMAAVMIGGMVPPLVIAIAADLFPQKFTAKERKDSKVNYLMGMAFITEGTIPYAASDPLRVIGSSIFASTLAGGMAAGLGCSIPAPHGGIFVFVVAQGWYWYLLALVVGAFAGAFILGALRKKVDPSELGRWKGIPIGNGVYLKRKVKQAQKVEK" }, "paralog": false, "identity": 0.9504950495049505, "score": 560.0, "evalue": 1.79e-202, "description": "Internal stop codon at 937119, 937131. Nonsense mutation around 937119, 937131.", "psc": { "uniref90_id": "UniRef90_C4XG39", "product": "PTS EIIC type-2 domain-containing protein", "uniref50_id": "UniRef50_C4XG39", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG39", "UniRef:UniRef50_C4XG39" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG39", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG39" ], "product": "PTS EIIC type-2 domain-containing protein" } }, "id": "OAIKOHHJFB_1677", "locus": "GCA000209735_01675" }, { "type": "cds", "sequence": "contig_1", "start": 937756, "stop": 938376, "strand": "-", "frame": 2, "gene": null, "product": "PTS system protein", "db_xrefs": [ "RefSeq:WP_013527001.1", "SO:0001217", "UniParc:UPI0001F59D3F", "UniRef:UniRef100_A0AB32XC71", "UniRef:UniRef50_A0A1I6IS36", "UniRef:UniRef90_A0AB32XC71" ], "nt": "GTGAAAATAAAAGAACTTCTAACTCTAAAAAATATTGATATTAATGCAAAAGCAAAAGATAAATTAGAAGCCATTGAATTAGCAGTTAATTTGATGGGTGATAACATTATAATTGACAAAAACGCCTATTTGACAGCGGTTTTGGAAAGAGAAAAACAAACAACAACTGGATTGGGCGATGAATTAGCATTGCCTCATGGACGCGGATCTTTCGTTAAGAAACCTCACTTAAGTGCACTTGTTTTAAAAGACGGAGTTGATTATCAATCTTTAGATAATAAACCTGTTAAATTGCTATTTCTAATTGCAGCTCCAGAAACAACAGATAGTGAAAACTTACACTTAGAAGTTTTGGCTAAATTGAGCAAAATGTTAGTTGATAAAGTATTTATTGAAAAATTAATTAATGCTAAAAGCAACAAAGAATTTCTAAAATTAATTGAAACAAAAGAACAAGAAGTTGATCAGAATGAATTTGAACAAACTAATAATGGTGAAAAATTTATAGTAGCTGTTACAGCTTGTCCAACTGGTATTGCTCATACATACATGGCTCAAGAATCTTTAGAAAATGCAGCTAAGGAAATGGGTTATCAAATCAAAGTTGAAACTCAAGGTTAA", "aa": "MKIKELLTLKNIDINAKAKDKLEAIELAVNLMGDNIIIDKNAYLTAVLEREKQTTTGLGDELALPHGRGSFVKKPHLSALVLKDGVDYQSLDNKPVKLLFLIAAPETTDSENLHLEVLAKLSKMLVDKVFIEKLINAKSNKEFLKLIETKEQEVDQNEFEQTNNGEKFIVAVTACPTGIAHTYMAQESLENAAKEMGYQIKVETQG", "aa_hexdigest": "75faa41b3cae68b5513e0370ab33114c", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001F59D3F", "ncbi_nrp_id": "WP_013527001.1", "uniref100_id": "UniRef100_A0AB32XC71", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59D3F", "RefSeq:WP_013527001.1", "UniRef:UniRef100_A0AB32XC71" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XC71", "uniref90_id": "UniRef90_A0AB32XC71" }, "psc": { "uniref90_id": "UniRef90_A0AB32XC71", "product": "PTS system protein", "uniref50_id": "UniRef50_A0A1I6IS36" }, "pscc": { "uniref50_id": "UniRef50_A0A1I6IS36", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1I6IS36" ], "product": "PTS system, fructose-specific IIC component" }, "genes": [], "id": "OAIKOHHJFB_1678", "locus": "GCA000209735_01676" }, { "type": "cds", "sequence": "contig_1", "start": 938373, "stop": 939287, "strand": "-", "frame": 3, "gene": "pfkB", "product": "1-phosphofructokinase", "db_xrefs": [ "EC:2.7.1.56", "GO:0008662", "SO:0001217", "UniRef:UniRef50_A0A3P8KBW0", "UniRef:UniRef90_C4XG41" ], "nt": "ATGATTTACACAGTGACTTTTTCACCAGCTATTGACTATACAATGTCTTTAGAGGGAAAAATAAATATAGGAGATATTAATCGCTCCAAAAGTGAAAATTATACAGTTGGTGGTAAAGGAATTAATGTTTCAATACTATTAAATAATTTAGGAACTAAAAATGTAGCTTTAGGTTTTGTCGGTGGTTTTACTGGTCAATATATTATTAATTACTTAAAAGATAAAAAAGTTAAAACAGATTTTTGTAATATTGAAGGTAATTCAAGAATTAATGTAAAACTAAATGGCAAAGCTAAAGAAACAGCTATTAATGCTAATGGACCTAAAATCAGTGATCAAAATTTAAAACAATTATTAGATAAGATTGCTTTTAATATTAAAGATAATGATACTTTAATTTTGGCTGGCAATGTTCCAAAAAATGTTGACACAAATGTTTATGAATTAATTTTAGAAAAACTCAAAAACAAAAGAATTTATATAATAGTAGATTCAACTAAACAATTTCTACTTAATACATTAAAATACAAACCATTTTTGATTAAACCTAATTTAGAAGAACTTGAAGAATTATTTGATGAAAAAGTTAAAACTAAAGAAGACATAAAAAGTCATGTTGAAAAACTTAAAAAATTAGGTGCCAGAAATGTGTTAGTGTCTTGTGGCGCTGAAGGAGCTTATTTATTTAGTGAAGATAATCAAACCTTTTATAGTGCTACATATAAAGGAAAAGTTGTAAGTACAGTTGGTTCAGGAGATAGCATGATTGCTGGATTTATTGATAATTATTTAAAAAATAAAGATTATAAACAAGCTTTAGAATTCTCAATAGCTTGTGGAGCAGCAACTGCTTTTGATAAAGAAATAGCTAATAAAAGTGCAATATTAGAATTACTTGAAAGGGGTCAAAAGTGA", "aa": "MIYTVTFSPAIDYTMSLEGKINIGDINRSKSENYTVGGKGINVSILLNNLGTKNVALGFVGGFTGQYIINYLKDKKVKTDFCNIEGNSRINVKLNGKAKETAINANGPKISDQNLKQLLDKIAFNIKDNDTLILAGNVPKNVDTNVYELILEKLKNKRIYIIVDSTKQFLLNTLKYKPFLIKPNLEELEELFDEKVKTKEDIKSHVEKLKKLGARNVLVSCGAEGAYLFSEDNQTFYSATYKGKVVSTVGSGDSMIAGFIDNYLKNKDYKQALEFSIACGAATAFDKEIANKSAILELLERGQK", "aa_hexdigest": "e5ee5bd9bfd88c9c6a56e7cc75693dae", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_C4XG41", "query_cov": 1.0, "subject_cov": 0.9934640522875817, "identity": 1.0, "score": 583.0, "evalue": 4.41e-210, "valid": true, "gene": "pfkB", "product": "1-phosphofructokinase", "ec_ids": [ "2.7.1.56" ], "uniref50_id": "UniRef50_A0A3P8KBW0", "go_ids": [ "GO:0008662" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KBW0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KBW0" ], "product": "1-phosphofructokinase" }, "genes": [ "pfkB" ], "id": "OAIKOHHJFB_1679", "locus": "GCA000209735_01677" }, { "type": "cds", "sequence": "contig_1", "start": 939430, "stop": 940173, "strand": "-", "frame": 2, "gene": null, "product": "HTH deoR-type domain-containing protein", "db_xrefs": [ "RefSeq:WP_013527003.1", "SO:0001217", "UniParc:UPI0001F59D40", "UniRef:UniRef100_C4XG42", "UniRef:UniRef50_C4XG42", "UniRef:UniRef90_C4XG42" ], "nt": "ATGCTTAAAGAACAAAGACTAGATGTTATTTTAAAAGAAGTAAATTCAAAAGGTGCTGCTTCACTAAAAGAGCTTGCAAAATTAACTCAAGCAAGCATTTCTTCAATACGCGAAGATGTTGCTGAATTAGATAAACAAAAATTATTAAAAAGAGTTCATGGCGGAGCTTATTCATTAAATAATAATTCTTCAGCAAATGATGTTGAATATGAATCAAGAAGTAATATTGAATTAGATGCAAAAATCAAATTAGCTACCAAAGCAAATGAATTTGTACAAGATAACTTATTAATTTTTATTGACTCAGGAACAACAACTCATCAATTAGCTAAATTAATTAATAACAAGAATGTAACTATAGTTACGAATTCAATTAATGTTGCTACAGAATTAAAAAGTCATTCAAACGTTACAACTATTTTAATAGGTGGATTATTAAAAAGTAATACGTTTAATGTTGTTGGATCATTAGCTTTAGATAATTTGAAAAACTTTAATTTTGATATTGGATTTATTGGAGCAAATGGATATAGCAATAATCTAGGTTTTACAACACCTGAAATTAATGAAAGTTTATTTAAAAAAGAAGCAATTTTAAAATGCAAAAAAAGTTATATTTTGTTAGACAAAACCAAAATAAATTCTGAATTTAAATATACTTTTGCAACCAATAATAAAAACATCACATTGATAACTAATTACAAGAAAAAAGATCTAAATCTTGAGAATATCATAGAAGTATAA", "aa": "MLKEQRLDVILKEVNSKGAASLKELAKLTQASISSIREDVAELDKQKLLKRVHGGAYSLNNNSSANDVEYESRSNIELDAKIKLATKANEFVQDNLLIFIDSGTTTHQLAKLINNKNVTIVTNSINVATELKSHSNVTTILIGGLLKSNTFNVVGSLALDNLKNFNFDIGFIGANGYSNNLGFTTPEINESLFKKEAILKCKKSYILLDKTKINSEFKYTFATNNKNITLITNYKKKDLNLENIIEV", "aa_hexdigest": "e1131629919161a5cbf6664cef52baec", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001F59D40", "ncbi_nrp_id": "WP_013527003.1", "uniref100_id": "UniRef100_C4XG42", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59D40", "RefSeq:WP_013527003.1", "UniRef:UniRef100_C4XG42" ] }, "ips": { "uniref100_id": "UniRef100_C4XG42", "uniref90_id": "UniRef90_C4XG42" }, "psc": { "uniref90_id": "UniRef90_C4XG42", "product": "HTH deoR-type domain-containing protein", "uniref50_id": "UniRef50_C4XG42" }, "pscc": { "uniref50_id": "UniRef50_C4XG42", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG42" ], "product": "HTH deoR-type domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_1680", "locus": "GCA000209735_01678" }, { "type": "cds", "sequence": "contig_1", "start": 940235, "stop": 940369, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59D41", "UniRef:UniRef100_A0AB32XCD5", "UniRef:UniRef50_A0AB32XCD5", "UniRef:UniRef90_A0AB32XCD5" ], "nt": "ATGAATAAAAATATAGATATGTCCTTAAACAATTTAGGGACATGTTTTTTTCTTATGTTCCAAATTGTAGATTTTAAAATGGGACATAATTTAATCCTTATGTCCCTAAAATTAAAAGAAATGTTTAATAATTAG", "aa": "MNKNIDMSLNNLGTCFFLMFQIVDFKMGHNLILMSLKLKEMFNN", "aa_hexdigest": "27e4ccb15e6ab8c8e5c6d05ec32e75af", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001F59D41", "uniref100_id": "UniRef100_A0AB32XCD5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59D41", "UniRef:UniRef100_A0AB32XCD5" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCD5", "uniref90_id": "UniRef90_A0AB32XCD5" }, "psc": { "uniref90_id": "UniRef90_A0AB32XCD5", "uniref50_id": "UniRef50_A0AB32XCD5" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XCD5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCD5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5200.235999999998, "isoelectric_point": 7.939620018005372 }, "id": "OAIKOHHJFB_1681", "locus": "GCA000209735_01679" }, { "type": "cds", "sequence": "contig_1", "start": 940403, "stop": 940552, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATTACAGGTCAAAAAACAATTCGTGATGTTATTGCATTTCCTAAGAATGCTAAAAACCAAGATGTTTTTACAGGAGCTCCAGGCAGTGTTGATAAAAAACAATTAGATGAATTATTCTTAGAAATTAAAAAAGAAGACAAATAA", "aa": "MIITGQKTIRDVIAFPKNAKNQDVFTGAPGSVDKKQLDELFLEIKKEDK", "aa_hexdigest": "07b496c0c7929d5adcab76461698f2aa", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5519.329399999999, "isoelectric_point": 8.034710884094238 }, "id": "OAIKOHHJFB_1682", "locus": "GCA000209735_01680" }, { "type": "cds", "sequence": "contig_1", "start": 940622, "stop": 940774, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGAAGGATGTTAGAGCGCTTTCATATGACTTAGTTTTAAATGGTTTTGAATTAGGTTCAGGTTCAGCAAGAATATTTGATAAAAAAACACAAAAGAAAATGTTTGATTTAATTGGTATGAGTGAAAAAGAACAACAATCAAGATTTGATTGA", "aa": "MKDVRALSYDLVLNGFELGSGSARIFDKKTQKKMFDLIGMSEKEQQSRFD", "aa_hexdigest": "c931cfcca338c9288d5a96698176e035", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 47, "aa_cov": 0.94, "hmm_cov": 1.0, "evalue": 6.779856570221793e-12, "score": 41.11616897583008, "start": 3, "stop": 49 } ], "seq_stats": { "molecular_weight": 5759.5484, "isoelectric_point": 8.065204429626462 }, "id": "OAIKOHHJFB_1683", "locus": "GCA000209735_01681" }, { "type": "cds", "sequence": "contig_1", "start": 940898, "stop": 941527, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGTGTTCAGGTTTTGAAAAATATTATCAATTTGCAAGATGTTTCAGAGATGAAGATAGTAGAAAAGATCGTCAACCAGAATTTACTCAATTAGATATTGAAACTTCATTCTTAAATGTTGAAGACTTTCAAAAATACATTGAAAAATTATTCAAACACATTTTTAAATCAATAGGAATTAAAATCAAGACTCCATTTCCAAGAGTTAAATATTTTGATGCTTTAAGAGATTATGGAACAGATAAGCCTGACTTAAGATATGATTATAAAATAGTTGACATCAATGACTTTTGCCTTAATACAGACTTTAATATCATTAAATATGCTCCAAGTAAAAGAATGCTTTTTGTAGATACTTTAATTACCAAAAAAGACTTTAAAGACTTAGAAGAAATTGCTAAGAAAAATAAAGCAAATATTCTATTCTACTTTGTTTATGAAAATGGTGAAATAGTTCACACCAACTTTGCAAACAAAGTTAAAGATGCAGCTTTAGAATTAGTCAAAAATAACAAAAACAAAAATGGTTCTTACTTTATAGTTGCTAATACTTATGATCACGCATCTCAAGCTTTAGGTGCAGTTAGAGTTGAATTAAATGAAAGATTTAATTATGCAAGAAAGGAATGA", "aa": "MCSGFEKYYQFARCFRDEDSRKDRQPEFTQLDIETSFLNVEDFQKYIEKLFKHIFKSIGIKIKTPFPRVKYFDALRDYGTDKPDLRYDYKIVDINDFCLNTDFNIIKYAPSKRMLFVDTLITKKDFKDLEEIAKKNKANILFYFVYENGEIVHTNFANKVKDAALELVKNNKNKNGSYFIVANTYDHASQALGAVRVELNERFNYARKE", "aa_hexdigest": "51c2a9d7caf6eb43fe2c14f49eb0fd24", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 185, "aa_cov": 0.8564593301435407, "hmm_cov": 0.7513513513513513, "evalue": 2.7277514901343404e-34, "score": 114.67032623291016, "start": 1, "stop": 179 } ], "seq_stats": { "molecular_weight": 24829.998800000016, "isoelectric_point": 8.735740089416506 }, "id": "OAIKOHHJFB_1684", "locus": "GCA000209735_01682" }, { "type": "cds", "sequence": "contig_1", "start": 941567, "stop": 942010, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25", "PFAM:PF01336.31" ], "nt": "ATGACTTTTATTGACTTAAGAGACCGCTATGGAATTACCCAATGTGTCTTTAAAACAGACTTAAACGTCACAAAAGAAAGTTCAATGGAAGTAATAGGGAAGGTTGTTTTAAGAAAACAAAAGAACCCTAATCTAGCTACTGGTGATATTGAAGTAGTAGTTGATAAATACACAATTTTTTCTGAAGCAAATGAAGAATTACCTTTTGCAATTAGAGATGATATTGATGTTAAAGAAGAAACCAGAATGAAATATCGTTTCTTAGATTTGCGTCGTCCAGTAATGCAACAAAACATTATCACTAAAGATAAAATTATGTTTGCTGTTAGAGAATTCATGCACCAAAATGATTTTATTGATATTGAAACTCCAATGCTTGCTAAAAGTACACCTGAAGGTGCTAGAGATTTTTTAGTACCTACTAGAAATAGAGATGAATTTTGA", "aa": "MTFIDLRDRYGITQCVFKTDLNVTKESSMEVIGKVVLRKQKNPNLATGDIEVVVDKYTIFSEANEELPFAIRDDIDVKEETRMKYRFLDLRRPVMQQNIITKDKIMFAVREFMHQNDFIDIETPMLAKSTPEGARDFLVPTRNRDEF", "aa_hexdigest": "f154b9af50561ab94a591ddd296e2f1d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 68, "aa_cov": 0.4557823129251701, "hmm_cov": 1.0, "evalue": 4.739670556987406e-23, "score": 77.75453186035156, "start": 78, "stop": 144 }, { "id": "PF01336.31", "name": "tRNA_anti-codon", "length": 65, "aa_cov": 0.4013605442176871, "hmm_cov": 0.8923076923076924, "evalue": 1.1891936528716992e-07, "score": 27.71599006652832, "start": 1, "stop": 59 } ], "seq_stats": { "molecular_weight": 17285.704099999988, "isoelectric_point": 5.311058235168456 }, "id": "OAIKOHHJFB_1685", "locus": "GCA000209735_01683" }, { "type": "cds", "sequence": "contig_1", "start": 942116, "stop": 943108, "strand": "-", "frame": 1, "gene": "hisS", "product": "histidine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.21", "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427", "KEGG:K01892", "SO:0001217", "UniRef:UniRef50_A0A0F5H223", "UniRef:UniRef90_C4XG44" ], "nt": "ATGTTTCGTTACGAACAACCACAAAAAGGACGTTATAGACAATTTAATCAAGCAGGGATGGAAATAGTTGATACAGTTATTAATCCATATAATGATGCTGAATTAATTATTGCAGCAGCTTCACTATTACAATGTCTTGATGTTAAATGTGTTTTAAAAATTAATTCAATTGGTGATGAAGAAACCAGAACTAGATATCAAGAAGAATTAAAAAAATATTTTGCTAAATATAAAGATCAATTAAATCCAATTAGTCTTGAAAGACTTGAAAATAATGTTTTAAGAATTCTTGATGATAAAGAAGAAAGGCAAAAAGATTTTGTTAAAAAAGCTCCTAAAATTAATAAATACTTAAGCAAAAACAGCACTGAATTTTTTAATAAATTAACTTCAATTTTAGACTTCAACAACATCAAATATCAAGTTGATTATTCTCTAGTTAGAGGACTTGATTATTATGATGAAGTTGTTTATGAATTTGTTTCAACTTCAAAAGAATCAGGAAGTCAAAGTACAATAATTGGCGGTGGAAGATATTCGAACTTAATCAAAGAATTAGGTGGACCTCAATTATATGCTTGCGGTTTTGGTTTTGGAGTAGATCGAGCAGCTGACATTATTAATGATGAAAACAAAGACAATAATGATGCTGAAAAAATAAACATTTTAGTAGCCACAACCAGTGAAAAAAATCTAGACATTTTATTTTCATTAACTAATGAGCTAAGAAATTATGGAAGCAAAATTGAATTTATTAAAGAAGTAGTTAAAAGCAAAAAAATCTTTGATAAGGCTCAAAAATTACAAGCTGATGTTGTGATTTTTGATGACAACATTCAAGGTCGTGAAACCTTTGTTGCTAAAAGTTTAAGCGATAATGATCGTATTATGTTCCAATACAATGAAGATGGTTATGCAGACTTATTAGACTTTTTAGCAGAACATAATTTACTAAGCGATTTAATAGCTGAAGAAGATGAAGACGAGGAATAA", "aa": "MFRYEQPQKGRYRQFNQAGMEIVDTVINPYNDAELIIAAASLLQCLDVKCVLKINSIGDEETRTRYQEELKKYFAKYKDQLNPISLERLENNVLRILDDKEERQKDFVKKAPKINKYLSKNSTEFFNKLTSILDFNNIKYQVDYSLVRGLDYYDEVVYEFVSTSKESGSQSTIIGGGRYSNLIKELGGPQLYACGFGFGVDRAADIINDENKDNNDAEKINILVATTSEKNLDILFSLTNELRNYGSKIEFIKEVVKSKKIFDKAQKLQADVVIFDDNIQGRETFVAKSLSDNDRIMFQYNEDGYADLLDFLAEHNLLSDLIAEEDEDEE", "aa_hexdigest": "164d7510889c1fd474910838282a6592", "start_type": "ATG", "rbs_motif": null, "genes": [ "hisS" ], "truncated": "3-prime", "pseudogene": { "start": 942116, "stop": 943408, "observations": { "mutation": [ 943153 ], "stop": [ 943153 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG44", "query_cov": 1.0, "subject_cov": 0.7268722466960352, "identity": 1.0, "score": 630.0, "evalue": 7.54e-226, "gene_start": 1, "gene_end": 330, "reference_start": 125, "reference_end": 454, "reference_sequence": "MYIIFNIKLSIYFRRHKMFNRIKGTRDFGPQENNVIELIRSAFVNYARNYNFHLIETPIIESATLYRRSVASSDIVKKEMYEFKDKGDRDIALRPEGTAGFVRALVENKWYATLKNTKFAYYGPMFRYEQPQKGRYRQFNQAGMEIVDTVINPYNDAELIIAAASLLQCLDVKCVLKINSIGDEETRTRYQEELKKYFAKYKDQLNPISLERLENNVLRILDDKEERQKDFVKKAPKINKYLSKNSTEFFNKLTSILDFNNIKYQVDYSLVRGLDYYDEVVYEFVSTSKESGSQSTIIGGGRYSNLIKELGGPQLYACGFGFGVDRAADIINDENKDNNDAEKINILVATTSEKNLDILFSLTNELRNYGSKIEFIKEVVKSKKIFDKAQKLQADVVIFDDNIQGRETFVAKSLSDNDRIMFQYNEDGYADLLDFLAEHNLLSDLIAEEDEDEE" }, "paralog": false, "identity": 0.9976744186046511, "score": 847.0, "evalue": 6.09e-314, "description": "Internal stop codon at 943153. Nonsense mutation around 943153.", "psc": { "uniref90_id": "UniRef90_C4XG44", "gene": "hisS", "product": "histidine--tRNA ligase", "ec_ids": [ "6.1.1.21" ], "uniref50_id": "UniRef50_A0A0F5H223", "kegg_orthology_id": "K01892", "go_ids": [ "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG44", "EC:6.1.1.21", "UniRef:UniRef50_A0A0F5H223", "KEGG:K01892", "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427" ] }, "pscc": { "uniref50_id": "UniRef50_A0A0F5H223", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0F5H223" ], "product": "Histidine--tRNA ligase" } }, "id": "OAIKOHHJFB_1686", "locus": "GCA000209735_01684" }, { "type": "cds", "sequence": "contig_1", "start": 943151, "stop": 943429, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13393.11" ], "nt": "ATGTTTAACCGAATTAAAGGAACAAGAGACTTTGGTCCACAAGAAAATAATGTGATTGAATTAATTAGAAGTGCATTTGTTAATTATGCAAGAAATTATAATTTCCATTTAATTGAAACTCCAATTATTGAATCAGCCACTCTTTATCGTCGTTCAGTTGCAAGTAGCGATATAGTTAAAAAAGAAATGTACGAATTTAAAGACAAAGGTGATCGTGATATTGCACTAAGACCAGAAGGAACAGCAGGTTTTGTTAGAGCTTTAGTTGAAAACAAATGA", "aa": "MFNRIKGTRDFGPQENNVIELIRSAFVNYARNYNFHLIETPIIESATLYRRSVASSDIVKKEMYEFKDKGDRDIALRPEGTAGFVRALVENK", "aa_hexdigest": "9a6234ea33c88e0ae5f1c82fd56bb223", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13393.11", "name": "tRNA-synt_His", "length": 84, "aa_cov": 0.8913043478260869, "hmm_cov": 0.9404761904761905, "evalue": 5.910259780529753e-10, "score": 34.91695785522461, "start": 7, "stop": 88 } ], "seq_stats": { "molecular_weight": 10642.96799999999, "isoelectric_point": 9.045768547058106 }, "id": "OAIKOHHJFB_1687", "locus": "GCA000209735_01685" }, { "type": "cds", "sequence": "contig_1", "start": 943595, "stop": 943768, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAGGCAAAAGATAATTTAAATCAAAAAGTTAATAAAAGAAAGAAAATCAGCTTCTTTACTGCTATGCTAATAGTAATTAGTGGCAGCAATGGTGCTGGTATTTATTTTCGTAGCGAAAGCATACTTAACAATTCACATCAGAGTTTAATTTTAGCTATCTTTTGTTGA", "aa": "MKKAKDNLNQKVNKRKKISFFTAMLIVISGSNGAGIYFRSESILNNSHQSLILAIFC", "aa_hexdigest": "4d61d16f94456fda6743f89e04ab57f4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6389.474499999997, "isoelectric_point": 10.227280616760254 }, "id": "OAIKOHHJFB_1688", "locus": "GCA000209735_01686" }, { "type": "cds", "sequence": "contig_1", "start": 943892, "stop": 944032, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAAAAACTTTATGACTTACTTATATTTGCCACTTAGTTATTTTTTAATGCCTCTTTATGCTTTAATGTCCTTACAACAAGGAGTAGGAGCAATATTAAATCAAGAAGCGTTTGTGATTGGCACTAAATATGATTGA", "aa": "MSKNFMTYLYLPLSYFLMPLYALMSLQQGVGAILNQEAFVIGTKYD", "aa_hexdigest": "13da91fbf543d50881c93dd170be8092", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5285.2212, "isoelectric_point": 5.946972846984862 }, "id": "OAIKOHHJFB_1689", "locus": "GCA000209735_01687" }, { "type": "cds", "sequence": "contig_1", "start": 944057, "stop": 944509, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGTAATTTATTTTTCAATAGTACCAGCTCTTTATACTAAAGCAGGTGATATTCAAAATAAAATTATGTTAGCTGTTAAATTTTTGCCTTTATTCTTTATTATCATTATTGGTTTTGTTTTGGCTTTTAGTTCAAAAGGTGAAGTAAATCAAACTCATTGGAATTTACAAGATCAACACTTTGATGTTAGATTGGGAAGTTCAATTGCTTCTTATAAATCTTTAGGTGCGGGCTTTGGAGTATTTTTATCAGTATCAGCTATCTTCTTTGTTTATGATGGTTTTTATATTTCAGCTGGTATCCAAAGCGAAATGAAAGAACCTCAAAAGACTCCTTTAGCATTATTATTTGGTTTATTGATTACCACAATAATTTATCTTTTAATTGCCATTTCAATGAGTATTAATGGTGGTTCATTTATTAAAATGAAGGATTATTTAAGTAACTGA", "aa": "MIVIYFSIVPALYTKAGDIQNKIMLAVKFLPLFFIIIIGFVLAFSSKGEVNQTHWNLQDQHFDVRLGSSIASYKSLGAGFGVFLSVSAIFFVYDGFYISAGIQSEMKEPQKTPLALLFGLLITTIIYLLIAISMSINGGSFIKMKDYLSN", "aa_hexdigest": "494bc9d6f6491d71f00d46eb57526dd8", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16623.47759999999, "isoelectric_point": 8.831669044494632 }, "id": "OAIKOHHJFB_1690", "locus": "GCA000209735_01688" }, { "type": "cds", "sequence": "contig_1", "start": 945387, "stop": 945758, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "TTGTTAATTCCCGGAGGTGGAGAGGCAAATATAAAATATCATAAAGGTAAATTTGTTACGGGAGATAATAGAATTGGACAAGTTCTAAATAAAAATGAAGTTGCAACAAAATTTTTATATTATTACTTTCTTTTAAATCTTGATTTAATTAGAAAAAATTTTAGAGGTGGTAGTATAAAGCATCCGTTTATGAAAAATATTTTGGAATTAAATATACCTATTCCTCCTTTAGAAACCCAAAACAAGATAGTTTCAATTCTTGACAAATTATCAGAATACTCTCAAGAAATTAATTTAGGTTTACCTGCCGAAATAGAATTAAGAAGTAAACAATTTAAGTATTACAGAGATCAATTATTAGATTTTAAATAA", "aa": "MLIPGGGEANIKYHKGKFVTGDNRIGQVLNKNEVATKFLYYYFLLNLDLIRKNFRGGSIKHPFMKNILELNIPIPPLETQNKIVSILDKLSEYSQEINLGLPAEIELRSKQFKYYRDQLLDFK", "aa_hexdigest": "837053176ece321e545d9cb7b07dfca1", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 97, "aa_cov": 0.7886178861788617, "hmm_cov": 0.9896907216494846, "evalue": 2.4422510510409054e-18, "score": 62.966827392578125, "start": 10, "stop": 106 } ], "seq_stats": { "molecular_weight": 14290.483499999986, "isoelectric_point": 9.396927833557129 }, "id": "OAIKOHHJFB_1691", "locus": "GCA000209735_01689" }, { "type": "cds", "sequence": "contig_1", "start": 946458, "stop": 946598, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTATTTACAAAAGTTATTAGATAAGTATTGTCCAGATGGATATGAGTGGGTTACATTGGGCGAAATATCAAGCATTAGGAGAGGTGCCTCTCCTAGACCTATATCTTCTTTTTTGTCTAAAGAAGGTTATCCATGA", "aa": "MSYLQKLLDKYCPDGYEWVTLGEISSIRRGASPRPISSFLSKEGYP", "aa_hexdigest": "7ae243b45b3552fb620c88577b9b2e05", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5239.9313999999995, "isoelectric_point": 7.869220542907715 }, "id": "OAIKOHHJFB_1692", "locus": "GCA000209735_01690" }, { "type": "cds", "sequence": "contig_1", "start": 946632, "stop": 947132, "strand": "-", "frame": 3, "gene": null, "product": "HTH domain protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D3MT65" ], "nt": "ATGATTTCTATATTTAAAGATAGAATTGAAATTGATTCTATGGGAAATTTAGGACCTGAACAAACAATAGAAGGTTTTTACAAAGGAGAATCAAAACCAGTTAATGAAAAGTTAGCCGAAATTTTTCTTCAATTAAGAATTAGTGAAAAATCAGGACGTGGAGTACCTGCAATAGTAAAAGTTTATGGCAAAGAAGCTATTTATTTTGGTGATGGATTTATTAAAGTTACAATTCCTTTTAATAGAATTAATGCTGTAAAATTTGAAATTGAAGTTGAAGAGATAGATGAAGAAACAGCTAAAAAAATTCTTAATCAAATAAGAAAAAAGATATTATCGGAAATTATTAAAAATCCACATATTACTAAAGCTTTATTAGCAGAAAAATTAAATCTAAGCACAACAGCAATAGACAACAATATTAATTTTTTAAAAAAACATGGTTTTATTAAAAGAGTTGGAGCAAATAAAAATGGTTATTGGAAGGCATTAATAAAATAA", "aa": "MISIFKDRIEIDSMGNLGPEQTIEGFYKGESKPVNEKLAEIFLQLRISEKSGRGVPAIVKVYGKEAIYFGDGFIKVTIPFNRINAVKFEIEVEEIDEETAKKILNQIRKKILSEIIKNPHITKALLAEKLNLSTTAIDNNINFLKKHGFIKRVGANKNGYWKALIK", "aa_hexdigest": "a49de4b4edd527e5d86e6c1466d6061b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_X8GT79", "query_cov": 0.9879518072289156, "subject_cov": 1.0123456790123457, "identity": 0.573, "score": 176.0, "evalue": 7.64e-54, "valid": false, "product": "Winged helix-turn-helix DNA-binding protein", "uniref50_id": "UniRef50_D3MT65" }, "pscc": { "uniref50_id": "UniRef50_D3MT65", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D3MT65" ], "product": "HTH domain protein" }, "genes": [], "id": "OAIKOHHJFB_1693", "locus": "GCA000209735_01691" }, { "type": "cds", "sequence": "contig_1", "start": 947151, "stop": 947408, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAGATAATTCTGGATTTCCAGTTAGAGTTTCTATTTTTGGAGGTTATGACAAAACAACCCCTTTATATACAGTAGATGAATTTGGATTTACTAATTTACTTTATACACTAGATGATTTGATTAGATTTGGAAATGTAGTAAATATTTATCAAGCTGATGAAAGAAACAGAGTTCTCACAAGAAAAGAAGTACCTTTATTTGATTCTAATGTTTACAAGGAAGCAATAATTAATGCCATCTTACATAACAAATGA", "aa": "MSDNSGFPVRVSIFGGYDKTTPLYTVDEFGFTNLLYTLDDLIRFGNVVNIYQADERNRVLTRKEVPLFDSNVYKEAIINAILHNK", "aa_hexdigest": "cda976f63e031c812f605beb8c012c8d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9763.917699999993, "isoelectric_point": 5.173166847229003 }, "id": "OAIKOHHJFB_1694", "locus": "GCA000209735_01692" }, { "type": "cds", "sequence": "contig_1", "start": 948180, "stop": 948671, "strand": "-", "frame": 3, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A257LJQ8" ], "nt": "ATGTCAGTTGTATTACAAGATTCATTTATGTTTAATGAAACCATTATGGACAACTTAAAAGTAGCTTCAGACAAAATCACAAACCAAGAAGTATATGAAATGGCTAGTTTAACTTCAGCTCATAACTTTATTCAAAAACTTGAAAAAGGTTATGACACTGTAATAGAAAATAGTGGTCAAAGTTTAAGTCAAGGTGAACGTCAATTGTTATCAATTACTCGGGCACTGTTGGGTAAAAAGAAAGTATTGATCTTAGATGAAGCCACAAGTAATGTTGATTCAAATACTGAACAAATTATTCAAAAAGCTTTACAAGAAGAATTAATGAAAGATCGTACTTCAATAGTTATAGCTCACCGTTTAAGTACTATTAAAAATGCGGACTTAATTTTAGTGGTTGATGATGGTCAAATTATTGAACAAGGAAATCATGCATCATTAATGGAAGCAAAAGGTTATTACTTTAATTTATATGAAAATCAATTCAAATAA", "aa": "MSVVLQDSFMFNETIMDNLKVASDKITNQEVYEMASLTSAHNFIQKLEKGYDTVIENSGQSLSQGERQLLSITRALLGKKKVLILDEATSNVDSNTEQIIQKALQEELMKDRTSIVIAHRLSTIKNADLILVVDDGQIIEQGNHASLMEAKGYYFNLYENQFK", "aa_hexdigest": "523b4f3bf27f526a669a4635f9842b70", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00261D2CD4", "query_cov": 0.9938650306748467, "subject_cov": 0.9700598802395209, "identity": 0.562, "score": 174.0, "evalue": 6.51e-53, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_A0A257LJQ8" }, "pscc": { "uniref50_id": "UniRef50_A0A257LJQ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A257LJQ8" ], "product": "ABC transporter domain-containing protein" }, "genes": [], "id": "OAIKOHHJFB_1695", "locus": "GCA000209735_01693" }, { "type": "cds", "sequence": "contig_1", "start": 949248, "stop": 949487, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTTTGTCGGTTTTACTTTAACAGCAATAGTAATTCCTTTATCAGTTTTATTCTTCTCACTTGGCTACTTCATGATTTCAAAAGCTAGAAAATCATATGTTCTTGTACAACAAGACTTTGGTGATTTAAATGCTTATGTTGAAGAAATGCTAACTAATAGCAAAATCACTCAAACTTTTGATCGTCAAGAAACAGCAGAAAAGAATTTCAAAAAAATAACTCATGATATTTATTGA", "aa": "MAFVGFTLTAIVIPLSVLFFSLGYFMISKARKSYVLVQQDFGDLNAYVEEMLTNSKITQTFDRQETAEKNFKKITHDIY", "aa_hexdigest": "fdfdb893b78e48f911fdba37f6ccc169", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9124.468399999996, "isoelectric_point": 6.541281318664551 }, "id": "OAIKOHHJFB_1696", "locus": "GCA000209735_01694" }, { "type": "cds", "sequence": "contig_1", "start": 949524, "stop": 949652, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAAACTTATTGAAATGCCAGTTAGCTTTTATGATAAAGAAAAAAGTGGTGACTTAATTTCAGTATTAATCAATGATATAAATAACTTAGCAAACTCATTTGTTCAAATGTTCAATGAAACTTGA", "aa": "MEKLIEMPVSFYDKEKSGDLISVLINDINNLANSFVQMFNET", "aa_hexdigest": "6178115e24ce4af06281e576f40eaf8d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4838.4865, "isoelectric_point": 4.196388816833497 }, "id": "OAIKOHHJFB_1697", "locus": "GCA000209735_01695" }, { "type": "cds", "sequence": "contig_1", "start": 950035, "stop": 950433, "strand": "-", "frame": 2, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DF8197" ], "nt": "ATGCGTGAAGCACTTAAAAATGCTTGTGCTGATGATTTTGTTAATAAATTTGAAGATGGTTTAGATCACAAAGTTGTTCAAGGAGCTAGAAACCTTAGCGGTGGTCAAAAACAAAGAATTTCAATAGCTCGTAGTTTATTAAGAAAACCTAAAATCTTAATTTTAGATGATTCAACAAGTGCACTAGACAATATTACAACTAGAAAAGTTATTAATAATATTAAAAATAATTATGACTGTTCAACAATATTAATTAGCCAAAAAATTGGAGCTATTAAAAATGCTGACAATATTATGGTTTTAACTAATGGAGCAGTAATGGATCAAGGTAAACACGGTCACTTAATTAAGACCTGCGATTTTTACCAAAAGATCTATGAAACACAGCTAGAACAATAG", "aa": "MREALKNACADDFVNKFEDGLDHKVVQGARNLSGGQKQRISIARSLLRKPKILILDDSTSALDNITTRKVINNIKNNYDCSTILISQKIGAIKNADNIMVLTNGAVMDQGKHGHLIKTCDFYQKIYETQLEQ", "aa_hexdigest": "49ff5100b3412023d25897d9a9573dda", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021DF8197", "query_cov": 1.0, "subject_cov": 0.8571428571428571, "identity": 0.614, "score": 167.0, "evalue": 1.01e-50, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI0021DF8197" }, "pscc": { "uniref50_id": "UniRef50_UPI0021DF8197", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DF8197" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "OAIKOHHJFB_1698", "locus": "GCA000209735_01696" }, { "type": "cds", "sequence": "contig_1", "start": 950461, "stop": 951057, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGATTTTTGCTTTTGCTGTACCGATTTTTTATGCTTCAATTAACTTATTAATTATTGGAATCTATGCTTTTGCAGGTCATAGAATGTATACTGAAGTTGCAACTTTAGATTCACTTGTTAAAGTTAATATTTTTATAGAATACTTATTTTCAATTAGCTTTGGTCTCTTAATGACCATTCAATTCTTAACATCAATGTTTAGAGCTCGTGTTTCAGCAGGTAGAATTAATGAAATTTTAGAAACCAAAATAGATCCTTTATTTATTAAAGATGGTAAACAATTAACAAATAATTTTGATCTAGAAGTTCAAAATCTTTCATTTCGTTACTATGAAACCAGCCCTTCATATTCACTAATGAATATAAATGTTAAATTACCTTATAAACAAACTTTAGGTATTATTGGACCAACAGGTAGTGGTAAAAGTACATTTATTAACTTACTTTTAAATAATTATGTTTATGATGAAGGTTCAATTAAAATTGGAGGCCAAGAAGTTAAAGAAATTAATACCAAAAACCTTCATGAAACTGTTGGCATAGTTTACCAAGAAGCTTTACTTTACACTGGAACAATTAGATCAAACTTACTTTGA", "aa": "MIFAFAVPIFYASINLLIIGIYAFAGHRMYTEVATLDSLVKVNIFIEYLFSISFGLLMTIQFLTSMFRARVSAGRINEILETKIDPLFIKDGKQLTNNFDLEVQNLSFRYYETSPSYSLMNINVKLPYKQTLGIIGPTGSGKSTFINLLLNNYVYDEGSIKIGGQEVKEINTKNLHETVGIVYQEALLYTGTIRSNLL", "aa_hexdigest": "b7d2e9fbc18ce98e3055e89f9cb0be74", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 77, "aa_cov": 0.3838383838383838, "hmm_cov": 1.0, "evalue": 8.653471961811733e-17, "score": 58.117149353027344, "start": 119, "stop": 194 } ], "seq_stats": { "molecular_weight": 22367.73180000001, "isoelectric_point": 6.737148475646973 }, "id": "OAIKOHHJFB_1699", "locus": "GCA000209735_01697" }, { "type": "cds", "sequence": "contig_1", "start": 951082, "stop": 951294, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCACTTAGTGTTTTAGCTGTTGTACCTGTAATTTTTGTACTAGTACTTGTTATTGGTTTAACTACTAATCCACTTTTAAAGAAAACACAAAAAGTTGTTGAAGAAATTACTAAAGCAACTGATGAAAATATTTTAGGAGCAAGAGTTATTAAAACTTTTAACTTAGAAGAAACAAGAAAAAAACGTTTTAGTGAATACAACCACAGATGA", "aa": "MSLSVLAVVPVIFVLVLVIGLTTNPLLKKTQKVVEEITKATDENILGARVIKTFNLEETRKKRFSEYNHR", "aa_hexdigest": "5035845b45203479abbec87401ce82ab", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7953.3474, "isoelectric_point": 9.773100852966305 }, "id": "OAIKOHHJFB_1700", "locus": "GCA000209735_01698" }, { "type": "cds", "sequence": "contig_1", "start": 951631, "stop": 951816, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAGTTAATTAAAATGCTACCAATTAAGATTAAATTAGCATTTCTTTTTGGTTCATTGCTTGTTTTTATTAATATTATTTTTACTTTGCTTTTGCCTAATATTATTTCTCAGTTTATTAGATTATTATTCGTTGATGATTATTCAAAAGTTGAGACATTATCCTTCTTTAATGGTCGTTGA", "aa": "MFKLIKMLPIKIKLAFLFGSLLVFINIIFTLLLPNIISQFIRLLFVDDYSKVETLSFFNGR", "aa_hexdigest": "316671a72d6d69cb3154fa8398c32412", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7154.733899999997, "isoelectric_point": 9.994614219665529 }, "id": "OAIKOHHJFB_1701", "locus": "GCA000209735_01699" }, { "type": "cds", "sequence": "contig_1", "start": 952159, "stop": 952392, "strand": "+", "frame": 1, "gene": null, "product": "Nudix hydrolase domain-containing protein", "db_xrefs": [ "EC:3.6.1.17", "KEGG:K01518", "SO:0001217", "UniRef:UniRef50_C4XG50", "UniRef:UniRef90_C4XG50" ], "nt": "ATGAAAGAAAAATATGTTTTACCTAATGGAATACCTAAAGAAGTTGTTTACTTTTTAGCAATGCAAACTGATAAAAAAGATCCTCAAAGACAAGAAAGCGAAATCTTAGAAATGAAATATTATTCATGTTCAAGAGCTATGAATAAATTAGTTCACAACAGTCAAAAAAGAGTTTTAAAAACAATGTATAAAAAGTTAAATCAATATTTGCTTGATGAAAAAAAGAATAAATAA", "aa": "MKEKYVLPNGIPKEVVYFLAMQTDKKDPQRQESEILEMKYYSCSRAMNKLVHNSQKRVLKTMYKKLNQYLLDEKKNK", "aa_hexdigest": "bd8e15c55a65609500915f50e1fb41a0", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 951931, "stop": 952392, "observations": { "mutation": [ 951946, 952009, 952045 ], "stop": [ 951946, 952009, 952045 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG50", "query_cov": 1.0, "subject_cov": 0.5032679738562091, "identity": 1.0, "score": 149.0, "evalue": 1.5e-44, "gene_start": 1, "gene_end": 77, "reference_start": 77, "reference_end": 153, "reference_sequence": "MFQKKWGLNMSEKSCGLVIFRQFTKGWKVLIAKQINNMWSFVKGHVENKESEIETALREAKEEVNLENFQIFKETRMKEKYVLPNGIPKEVVYFLAMQTDKKDPQRQESEILEMKYYSCSRAMNKLVHNSQKRVLKTMYKKLNQYLLDEKKNK" }, "paralog": false, "identity": 0.9738562091503268, "score": 293.0, "evalue": 9.56e-104, "description": "Internal stop codon at 951946, 952009, 952045. Nonsense mutation around 951946, 952009, 952045.", "psc": { "uniref90_id": "UniRef90_C4XG50", "product": "Nudix hydrolase domain-containing protein", "ec_ids": [ "3.6.1.17" ], "uniref50_id": "UniRef50_C4XG50", "kegg_orthology_id": "K01518", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG50", "EC:3.6.1.17", "UniRef:UniRef50_C4XG50", "KEGG:K01518" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG50", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG50" ], "product": "Nudix hydrolase domain-containing protein" } }, "id": "OAIKOHHJFB_1702", "locus": "GCA000209735_01700" }, { "type": "cds", "sequence": "contig_1", "start": 952472, "stop": 952975, "strand": "-", "frame": 1, "gene": "pgmB", "product": "beta-phosphoglucomutase", "db_xrefs": [ "EC:5.4.2.6", "GO:0000287", "GO:0005975", "GO:0008801", "SO:0001217", "UniRef:UniRef50_A0A5B8JY41", "UniRef:UniRef90_A0A3P8K8F6" ], "nt": "TTGCCTCGTAGAGATACTTTACTTGCAATATTTGAATTAAAGAAATTTAAGCATAATTTTGATGATCAAAAAATCAATGAATTATGTACTAAAAAGAATGAATTATATTTAAGTTATTTAAAAAAATATATTAATAAAGATAGCCTTTTAGAAGGCATCGAACAATTTTTAAAAGACTTAAAAAAAGATAAGATTAAAATTGCAATAGCTTCTTCAAGTCTCAATGCTCCTTTGATTTTAGAAAAACTCGGAGTTATAAATTATTTTGATGTTATTGTTAATCCTGTTGAAGTTAAAAAAGGAAAACCTGCACCAGATATTTTTCTTTTAGCTCAAGAACAACTTAAATTAAAAAAAGAAGAATGTGTTGGAATTGAAGATGCAGTTGTTGGAGTTGAAGCTTTAAATAAGGCTAACATTAAATCCATTGCAATAACAAACGGTAATAATAATTTATTCCAAAAAGCAACTTTATTAGTTCAAGACACTTCTAAATTAATTTGA", "aa": "MPRRDTLLAIFELKKFKHNFDDQKINELCTKKNELYLSYLKKYINKDSLLEGIEQFLKDLKKDKIKIAIASSSLNAPLILEKLGVINYFDVIVNPVEVKKGKPAPDIFLLAQEQLKLKKEECVGIEDAVVGVEALNKANIKSIAITNGNNNLFQKATLLVQDTSKLI", "aa_hexdigest": "62ea9c14ed358c8169efc4738cc1a76d", "start_type": "TTG", "rbs_motif": null, "genes": [ "pgmB" ], "truncated": "both", "pseudogene": { "start": 952445, "stop": 953167, "observations": { "mutation": [ 952474, 953041, 953116 ], "stop": [ 952474, 953041, 953116 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8K8F6", "query_cov": 1.0, "subject_cov": 0.6958333333333333, "identity": 0.9940000000000001, "score": 318.0, "evalue": 1.55e-108, "gene_start": 1, "gene_end": 167, "reference_start": 65, "reference_end": 231, "reference_sequence": "MLMVWTVKLQMKSKFSRWKMIKGIIFDLDGVITDTAKLHYKAWKEIVNELGIDYSKEENEKLRGLPRRDTLLAIFELKKFKHNFDDQKINELCTKKNELYLSYLKKYINKDSLLEGIEQFLKDLKKDKIKIAIASSSLNAPLILEKLGVINYFDVIVNPVEVKKGKPAPDIFLLAQEQLKLKKEECVGIEDAVVGVEALNKANIKSIAITNGNNNLFQKATLLVQDTSKLIWDLIKPILF" }, "paralog": false, "identity": 0.9833333333333333, "score": 454.0, "evalue": 7.69e-165, "description": "Internal stop codon at 952474, 953041, 953116. Nonsense mutation around 952474, 953041, 953116.", "psc": { "uniref90_id": "UniRef90_A0A3P8K8F6", "gene": "pgmB", "product": "beta-phosphoglucomutase", "ec_ids": [ "5.4.2.6" ], "uniref50_id": "UniRef50_A0A5B8JY41", "go_ids": [ "GO:0000287", "GO:0005975", "GO:0008801" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8K8F6", "EC:5.4.2.6", "UniRef:UniRef50_A0A5B8JY41", "GO:0000287", "GO:0005975", "GO:0008801" ] }, "pscc": { "uniref50_id": "UniRef50_A0A5B8JY41", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A5B8JY41" ], "product": "Beta-phosphoglucomutase" } }, "id": "OAIKOHHJFB_1703", "locus": "GCA000209735_01701" }, { "type": "cds", "sequence": "contig_1", "start": 953409, "stop": 954074, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03632.21" ], "nt": "ATGAAAAAAATAGGTTATGAAATGATAATTGATACAGCTTACTTTTACACAAACCGTGCAGAAATACAACCAGATGGTAGTTATGAAATTAAAGATGTAATGGGTCCAAATGAATATAAAGGAAACATTAATAATAATGCATTTATCAACCAAATGGCCTTATTTAATATGAAATTGGCAATTTCAATATATCGCAAATTGCAAGCACAAACACCAGGCTTACTTAACTGTATTTTAAAAAGAATACCTTATAAAATTAATTTTTCAAAAATGACTAAAGTCATTAAAGGTCTTAAAATTCAAGCTCCTAATAAAGATAAAATTATTGCAGAAAATGATACATTCTTATCCTTACCTTTAAAAAATGTTAGACCATTTCAAATGCTTGGAGATGCTGGTAAAAAATTATTCAATACTTCTGAAGGACATAGATTATTAGGCTCTCAATTAGTAAAACAAGCCGATGTCATTCTCTTAACTTATTTATTCCCTGAGTTATATAATAAAGAAATTAGAGAAAAAAACTTTGATTACTATGAAGCAATAACCACACATGATTCATCACTTTCGGCAGCAACTTATTGTATTGAAGCTATTAGATTAAATAAAATTGAAAAAGCTTATGAATTATTTAAATATGGTATTAATATTGACTTTGGTCCATGA", "aa": "MKKIGYEMIIDTAYFYTNRAEIQPDGSYEIKDVMGPNEYKGNINNNAFINQMALFNMKLAISIYRKLQAQTPGLLNCILKRIPYKINFSKMTKVIKGLKIQAPNKDKIIAENDTFLSLPLKNVRPFQMLGDAGKKLFNTSEGHRLLGSQLVKQADVILLTYLFPELYNKEIREKNFDYYEAITTHDSSLSAATYCIEAIRLNKIEKAYELFKYGINIDFGP", "aa_hexdigest": "30b817bdc780bf80d9fe33155f5fcfe2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03632.21", "name": "Glyco_hydro_65m", "length": 221, "aa_cov": 0.9909502262443439, "hmm_cov": 0.9638009049773756, "evalue": 1.0116710259640507e-55, "score": 185.2943115234375, "start": 1, "stop": 219 } ], "seq_stats": { "molecular_weight": 25390.247700000007, "isoelectric_point": 9.173480415344237 }, "id": "OAIKOHHJFB_1704", "locus": "GCA000209735_01702" }, { "type": "cds", "sequence": "contig_1", "start": 954576, "stop": 955472, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATATATTGAATATGACCCTGATAATAATCAGATTTCACAGATTAAATTTGACCGTAATATAACAGCAAAAAGTGAAAGTATTTTTTCACTAGGTAATGGTTATTTAGGTATTAGAAGTGTTGATGAAGAAGTTCAAGAATATAACAAAGAAGACTTTTTTGTTAATGGTATTTTTAATAGAACATCTAAAGGAGAAGTTCCTGAATTAGCTAATTTAGCTGATTTAATTCAAACTCCTATTTCTTTTAATAATCAAATTTTTGAAGTTAATGAAAAGGATCAATATACTAAAACTTTAGATATTAAAAATGGAATTTTAAGTCGTAAAGTCATTGCCAAAAGAGACTTTGCTACAATTGAATTAAATTTTGAACGTTTTGTTTCTCAAGAAATAAGAAACATTTATGCTCAAAAAATTACAATTAAAATTTTAGATTCTAAAGATGAAATTGTTAAGGTTAAATTATTACCAGGAATTAATGGACAAGTAACAAACAGTGGTACTCAACACTTTGAAGAAGGTCTTAAAAACCGTGTGACAACCGAAAGTTTGAAGATGCAACAAATAACAACTAATTCAAATCGTTTTGTTGTGCACCACATGTTTACAAGACTTTTAAAAAATAATAAGTTAATTTTAGGCAGTAATGACAAATATCAAATTCAAACTGATCGCCGCAAAGTTTACTTTAAAATCGAAAATGAATTAAAAGCAAACGAAGAATTAGTTTTAGAAAAATTAATGTCAGTTCATACATCAATTGATGATGAAGAAAAAATATTAACTAATAGTGAAGTTTTGGCTAAATCAGATTTAACTTTTAACTACTTATTAAAAAATACATATGCTTCATTAAAAGAAAATTCTACAGCTGCAATGCAAAAAGTTTGA", "aa": "MEYIEYDPDNNQISQIKFDRNITAKSESIFSLGNGYLGIRSVDEEVQEYNKEDFFVNGIFNRTSKGEVPELANLADLIQTPISFNNQIFEVNEKDQYTKTLDIKNGILSRKVIAKRDFATIELNFERFVSQEIRNIYAQKITIKILDSKDEIVKVKLLPGINGQVTNSGTQHFEEGLKNRVTTESLKMQQITTNSNRFVVHHMFTRLLKNNKLILGSNDKYQIQTDRRKVYFKIENELKANEELVLEKLMSVHTSIDDEEKILTNSEVLAKSDLTFNYLLKNTYASLKENSTAAMQKV", "aa_hexdigest": "a1b45e26078bfdf17cfe32092316992b", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 34425.60590000004, "isoelectric_point": 5.952997779846191 }, "id": "OAIKOHHJFB_1705", "locus": "GCA000209735_01703" }, { "type": "cds", "sequence": "contig_1", "start": 955482, "stop": 955607, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAACATCAAAAAGTAGTTGAAAATGTTCAAGGAAAACAAATATTATCAACATATATTGATAACAAAAAAGTTACTGATAAATTAAGACCTTTTGAGTCGATTATGTTAGAAATTAAATAA", "aa": "MTKHQKVVENVQGKQILSTYIDNKKVTDKLRPFESIMLEIK", "aa_hexdigest": "f0dee4995cbcbd89a904d5f347d56a20", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4831.654999999999, "isoelectric_point": 9.52289905548096 }, "id": "OAIKOHHJFB_1706", "locus": "GCA000209735_01704" }, { "type": "cds", "sequence": "contig_1", "start": 955872, "stop": 956087, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00128.31" ], "nt": "ATGCTTTTTGGGCTAAAAGGTATTCCTTGTATTTATTATGGAGAAGAAATTGGTTTATTAAATAATCAATTTAACTCAAGAGAAGAATTTAAAGATTGTGACATATTTAATGCCTTTAGTGAGTTAGTAGATAAAAAGAAAATTTACAGTGAATACGAATTTATTAAATATTGCAATATCAACAGTCGTGACTCAGGCCGCACTTTGATGCAATGA", "aa": "MLFGLKGIPCIYYGEEIGLLNNQFNSREEFKDCDIFNAFSELVDKKKIYSEYEFIKYCNINSRDSGRTLMQ", "aa_hexdigest": "b957924e0ae6890f9dff0f674016da08", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00128.31", "name": "Alpha-amylase", "length": 70, "aa_cov": 0.9859154929577465, "hmm_cov": 0.7285714285714285, "evalue": 1.769886294384702e-07, "score": 26.826805114746094, "start": 2, "stop": 71 } ], "seq_stats": { "molecular_weight": 8391.477099999996, "isoelectric_point": 4.990997886657714 }, "id": "OAIKOHHJFB_1707", "locus": "GCA000209735_01705" }, { "type": "cds", "sequence": "contig_1", "start": 956307, "stop": 956453, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTCTTAAGTTTAATAAGTTAGCTTTTAGTGATAAAAAAGATGCTTATACATTAGGTGAAGCTAGTGGAATTAATTTAGAAGAATTATTAAAATATGGAACAGGATCTAAAAAAGTAGCTGATAACTATTACAACTTTTCTTGA", "aa": "MLLKFNKLAFSDKKDAYTLGEASGINLEELLKYGTGSKKVADNYYNFS", "aa_hexdigest": "b6d4506498b4c2104deb739d609a6e3c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5397.071800000002, "isoelectric_point": 7.961668205261231 }, "id": "OAIKOHHJFB_1708", "locus": "GCA000209735_01706" }, { "type": "cds", "sequence": "contig_1", "start": 956979, "stop": 957125, "strand": "-", "frame": 3, "gene": null, "product": "alpha-amylase family glycosyl hydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021FD1789" ], "nt": "ATGAAAACAATAAAATTAGAAGATAAAATTATTTACCAAATATTTCCTCGTTCATTTTATGACTCAAATAATGATGGCGATGGAGACTTATTAGGTATAGCAAAAAAACTAGATTATTTACAAGACTTAGGTATAAATGCGATATGA", "aa": "MKTIKLEDKIIYQIFPRSFYDSNNDGDGDLLGIAKKLDYLQDLGINAI", "aa_hexdigest": "53bacd49ab9424190d5d678759b2dd5e", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0021FD1789", "query_cov": 1.0, "subject_cov": 0.8571428571428571, "identity": 0.7709999999999999, "score": 80.1, "evalue": 5.15e-19, "valid": false, "product": "alpha-amylase family glycosyl hydrolase", "uniref50_id": "UniRef50_UPI0021FD1789" }, "pscc": { "uniref50_id": "UniRef50_UPI0021FD1789", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021FD1789" ], "product": "alpha-amylase family glycosyl hydrolase" }, "genes": [], "id": "OAIKOHHJFB_1709", "locus": "GCA000209735_01707" }, { "type": "cds", "sequence": "contig_1", "start": 957469, "stop": 957675, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTTGCAAATTACAACACAAGGACGTCAATTCTTTTTAGCAGGTACTGAATTAGCTCAAAGTAAACCTGCTGATAAAAGTGGTCAAGATTTTCATAAGTCATTTTATTCATATGCCAAAGATTATTTTGAATTAAATTCAGATGAAAATAAATACCATTTTAATTCTTATAAAACGACAGATTATACTAACGGACTAAAATGA", "aa": "MILQITTQGRQFFLAGTELAQSKPADKSGQDFHKSFYSYAKDYFELNSDENKYHFNSYKTTDYTNGLK", "aa_hexdigest": "14bc447c73774bc7e88605fb093b9d0e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7929.621499999998, "isoelectric_point": 6.706910133361816 }, "id": "OAIKOHHJFB_1710", "locus": "GCA000209735_01708" }, { "type": "cds", "sequence": "contig_1", "start": 958060, "stop": 958332, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF9781" ], "nt": "ATGATGATAAATTCAATTTATGATAATATTCTGCCTGGTTACTATTATCGAGATAATGCTAAAGTAAAACCTGTTATTTATCCGCCTTTAGCAAGTCAAAGATATATGGTTAGAAAACTAATTTATGAATCACTAAAACATTTTGTTGAATATTATGATGTTGATGGTTTCCGTTTTGATTTATCAAGTTTTATTGATAAAGAAACACTTGATTATTGTTTTAAAAACTTAAGAAAAATAAAACCTAATTTAGTAATCCATGGTGAAGCTTGA", "aa": "MMINSIYDNILPGYYYRDNAKVKPVIYPPLASQRYMVRKLIYESLKHFVEYYDVDGFRFDLSSFIDKETLDYCFKNLRKIKPNLVIHGEA", "aa_hexdigest": "22ce29c00e40eccfa8031a13fb171c4b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002540EC32", "query_cov": 0.9444444444444444, "subject_cov": 0.8854166666666666, "identity": 0.6940000000000001, "score": 123.0, "evalue": 7.28e-35, "valid": false, "uniref50_id": "UniRef50_UPI0021AF9781" }, "pscc": { "uniref50_id": "UniRef50_UPI0021AF9781", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF9781" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10795.404299999991, "isoelectric_point": 8.62549915313721 }, "id": "OAIKOHHJFB_1711", "locus": "GCA000209735_01709" }, { "type": "cds", "sequence": "contig_1", "start": 959050, "stop": 959166, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATATAAAAGGACAAACTAAAGAATTTTTTAAGGACTTTGATGCTAAATATGCTTATAAAAAAGCTGATTTAGGAACAAAATTCCATAAAAATCATATTGAAATAAAATTTTGA", "aa": "MNIKGQTKEFFKDFDAKYAYKKADLGTKFHKNHIEIKF", "aa_hexdigest": "47b64872272290e3a6bff20cd568ba2e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4581.255099999999, "isoelectric_point": 9.574667167663574 }, "id": "OAIKOHHJFB_1712", "locus": "GCA000209735_01710" }, { "type": "cds", "sequence": "contig_1", "start": 959312, "stop": 959665, "strand": "-", "frame": 1, "gene": null, "product": "HTH gntR-type domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RW07", "UniRef:UniRef50_UPI0021AECA4B", "UniRef:UniRef90_C4XG55" ], "nt": "ATGGTAGATAAAAGAATTCTCTTTGTTCAAGGTTTTAGAACTTTTGAAAAAACTTATTATAAAGATAATGAGGTTTTAGCAACTTCTGAAATTTATGTAAGCTGTAAAAATATTGAAGTTGATGAGGAAATTAATTTTAATAAATCTTTAACCGACTTATTAATTGAGCGCGATGGTTTAAATAATATGGTTTATTCATTATCTTATGAAAATGTTAAAAAATTTGGATATGAAAGCTTGGTAGTTGTTTCATTTTTTGGCTATGATGAAGATAGCATTTCTGTAGCTGGAAAGTATTATATTAATCCTAAATACTTTAGATTTTTCCATCAAGAATTTTCATTAAAATCATAA", "aa": "MVDKRILFVQGFRTFEKTYYKDNEVLATSEIYVSCKNIEVDEEINFNKSLTDLLIERDGLNNMVYSLSYENVKKFGYESLVVVSFFGYDEDSISVAGKYYINPKYFRFFHQEFSLKS", "aa_hexdigest": "63039c7ad349478c37a49bd95f022305", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_UPI0021AECA4B", "query_cov": 0.9658119658119658, "subject_cov": 0.8828125, "identity": 0.513, "score": 123.0, "evalue": 5.44e-34, "valid": false, "uniref50_id": "UniRef50_UPI0021AECA4B" }, "pscc": { "uniref50_id": "UniRef50_UPI0021AECA4B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AECA4B" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 959312, "stop": 959965, "observations": { "mutation": [ 959668 ], "stop": [ 959668 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG55", "query_cov": 1.0, "subject_cov": 0.5, "identity": 1.0, "score": 232.0, "evalue": 1e-75, "gene_start": 1, "gene_end": 117, "reference_start": 118, "reference_end": 234, "reference_sequence": "MFLKFKGVYNIYIMDLQEKKKTQVIIDYLIDLIKTQKVPTNKILPSEHSLMMRFDCSRSIVVKAYQKLEALGAIYSISKRGHFVAENFHNLIKPISYLLGATAQEGKEIKSKELPQWMVDKRILFVQGFRTFEKTYYKDNEVLATSEIYVSCKNIEVDEEINFNKSLTDLLIERDGLNNMVYSLSYENVKKFGYESLVVVSFFGYDEDSISVAGKYYINPKYFRFFHQEFSLKS" }, "paralog": false, "identity": 0.9953917050691244, "score": 427.0, "evalue": 5.88e-155, "description": "Internal stop codon at 959668. Nonsense mutation around 959668.", "psc": { "uniref90_id": "UniRef90_C4XG55", "product": "HTH gntR-type domain-containing protein", "uniref50_id": "UniRef50_L0RW07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG55", "UniRef:UniRef50_L0RW07" ] }, "pscc": { "uniref50_id": "UniRef50_L0RW07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RW07" ], "product": "HTH gntR-type domain-containing protein" } }, "id": "OAIKOHHJFB_1713", "locus": "GCA000209735_01711" }, { "type": "cds", "sequence": "contig_1", "start": 959666, "stop": 959977, "strand": "-", "frame": 1, "gene": "gntR", "product": "GntR family transcriptional regulator", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021E0F715" ], "nt": "ATGGATTTACAAGAAAAGAAGAAAACACAAGTGATTATTGATTATTTAATTGATTTGATTAAAACTCAAAAAGTACCAACAAACAAAATCTTGCCAAGTGAGCATTCTTTAATGATGCGTTTTGATTGTTCAAGAAGTATTGTTGTTAAAGCATATCAAAAATTAGAAGCATTAGGAGCTATCTATTCAATTTCAAAAAGAGGACATTTTGTTGCTGAAAACTTTCACAACTTAATTAAACCAATTAGTTATCTTTTAGGAGCAACAGCTCAAGAAGGAAAAGAAATAAAAAGCAAGGAATTACCTCAATGA", "aa": "MDLQEKKKTQVIIDYLIDLIKTQKVPTNKILPSEHSLMMRFDCSRSIVVKAYQKLEALGAIYSISKRGHFVAENFHNLIKPISYLLGATAQEGKEIKSKELPQ", "aa_hexdigest": "31cec69671252884f422fec175262b5a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FF0E19D", "query_cov": 0.8058252427184466, "subject_cov": 0.8137254901960784, "identity": 0.711, "score": 127.0, "evalue": 2.17e-36, "valid": false, "product": "GntR family transcriptional regulator", "uniref50_id": "UniRef50_UPI0021E0F715" }, "pscc": { "uniref50_id": "UniRef50_UPI0021E0F715", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021E0F715" ], "product": "GntR family transcriptional regulator" }, "genes": [ "gntR" ], "id": "OAIKOHHJFB_1714", "locus": "GCA000209735_01712" }, { "type": "cds", "sequence": "contig_1", "start": 960075, "stop": 960335, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32EFF", "UniRef:UniRef90_UPI0001E32EFF" ], "nt": "ATGATTGCTTTAATTAATAAGCTAAGAAAAGATTATTCTTTTTTAAGTTTAACTAATATAGAAACTTTAGTTGATCCAAGATTAGTAGTAGACAGTAAAGATTATTCAAATGTTATTGTCCGTAAAGATCTTAATGATGATAAGAATTATTTAATGTTTGTTATTTACAATCATAAATGTCAAAATAATGTTCTTCAAAAGATTAGCAGGAATTATCACTTTGAAGCATTATATGAATATAAATGCAAAAATATGCCATGA", "aa": "MIALINKLRKDYSFLSLTNIETLVDPRLVVDSKDYSNVIVRKDLNDDKNYLMFVIYNHKCQNNVLQKISRNYHFEALYEYKCKNMP", "aa_hexdigest": "07270961a4e46e10aa869bdcf8d83666", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 960021, "stop": 960581, "observations": { "mutation": [ 960077, 960470 ], "stop": [ 960077, 960470 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001E32EFF", "query_cov": 1.0, "subject_cov": 0.46236559139784944, "identity": 0.919, "score": 156.0, "evalue": 5.41e-47, "gene_start": 1, "gene_end": 86, "reference_start": 83, "reference_end": 168, "reference_sequence": "MLYLLALPAKPIIYYGDELAYAGTRSFDDPGLREPMKWKNHQEISAIYEKKLDACQSNLFLNVSNSLDYVENEIAPGNSIYNMIALINKLRKEYSFLSLTNIETLVDPRLVVDSKDYSNVIVRKDLNDDKNYLMFVIYNHKCQNNVLQKISRNYHFEALYEYKCKNMPWGIIMQKYACGIYKLTKK" }, "paralog": false, "identity": 0.956989247311828, "score": 353.0, "evalue": 3.85e-127, "description": "Internal stop codon at 960077, 960470. Nonsense mutation around 960077, 960470.", "psc": { "uniref90_id": "UniRef90_UPI0001E32EFF", "uniref50_id": "UniRef50_UPI0001E32EFF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001E32EFF", "UniRef:UniRef50_UPI0001E32EFF" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32EFF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32EFF" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10322.852299999997, "isoelectric_point": 8.886080360412599 }, "id": "OAIKOHHJFB_1715", "locus": "GCA000209735_01713" }, { "type": "cds", "sequence": "contig_1", "start": 960468, "stop": 960581, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTTATCTTTTAGCTTTGCCTGCTAAACCAATAATTTATTATGGTGATGAATTAGCATATGCAGGAACTAGAAGCTTTGATGATCCTGGTTTAAGAGAACCAATGAAGTGA", "aa": "MLYLLALPAKPIIYYGDELAYAGTRSFDDPGLREPMK", "aa_hexdigest": "389e5d2b2e414406c4f45697653c71b4", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4189.8484, "isoelectric_point": 4.8610639572143555 }, "id": "OAIKOHHJFB_1716", "locus": "GCA000209735_01714" }, { "type": "cds", "sequence": "contig_1", "start": 961692, "stop": 961856, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATACATCAAGATTTTTATTTAAAAAATATTTTAATATTTAAAAAAGTTAAAATTATTATTATGAAAACAAACAAATTAAAAGAAAAATTTATGAACTTTATTAAAAAAATGAAACTTAAAAAATTCATTAAAAAACATCCAAACCAAAATTATGTATCTTGA", "aa": "MIHQDFYLKNILIFKKVKIIIMKTNKLKEKFMNFIKKMKLKKFIKKHPNQNYVS", "aa_hexdigest": "6e65700a5dc70567e8ee1bf50ff87fcf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6714.3002000000015, "isoelectric_point": 10.3802640914917 }, "id": "OAIKOHHJFB_1717", "locus": "GCA000209735_01715" }, { "type": "cds", "sequence": "contig_1", "start": 961885, "stop": 962013, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCGTTATACTAACAAACTTTATTTTGTTAATTGTTGCTAAAACAAAATATGGATATGTTAAAAATGACCAAATTATTGCTAAAAGCAAAATTGATACTAAGGACTTAGAAAAACAATTAATATAA", "aa": "MFVILTNFILLIVAKTKYGYVKNDQIIAKSKIDTKDLEKQLI", "aa_hexdigest": "9a1f1b8edc24a089397c55dd38a791a3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4870.8315, "isoelectric_point": 9.45688343048096 }, "id": "OAIKOHHJFB_1718", "locus": "GCA000209735_01716" }, { "type": "cds", "sequence": "contig_1", "start": 962020, "stop": 962277, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAATATAAATATTCTCTTCATATCTTTAATCATTAATTGTATTGCAGTAATTCGACTTTTCTCAAATAAAAGTAATGCAGACAAATACTTTATTTATCTTATATTTCTCCAATTAACCTCTTTTTGTTTATTCTTTTTTGCAATCTCACTAAGTCCTAATAAACTTAACAAAGTTATTGACTTTAAAGAATTAAGCGGTTCAGAATATGCTATATATCAATTTTTAGATAAAATGAACATTACTTATGCATGA", "aa": "MININILFISLIINCIAVIRLFSNKSNADKYFIYLIFLQLTSFCLFFFAISLSPNKLNKVIDFKELSGSEYAIYQFLDKMNITYA", "aa_hexdigest": "9cb1d343e5f21775a9d36a7a46107721", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9907.670199999991, "isoelectric_point": 8.617247200012208 }, "id": "OAIKOHHJFB_1719", "locus": "GCA000209735_01717" }, { "type": "cds", "sequence": "contig_1", "start": 962389, "stop": 962547, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCAAAGTTAACCAAAAATTAAAAAAATCTAAAACAAATTATTTAGCTCGTAAATCTTTATTAGTATTATCAATTATTATTACAATAGTTGCAATAGCAATATTGTTTGCTTTTCTCTTTTTATTCTTAAAAAGAATAGATGAAGAATTAAATTGA", "aa": "MFKVNQKLKKSKTNYLARKSLLVLSIIITIVAIAILFAFLFLFLKRIDEELN", "aa_hexdigest": "c36ec00f5fb637ce26c7203e8208a0b5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6070.406899999995, "isoelectric_point": 10.214515876770019 }, "id": "OAIKOHHJFB_1720", "locus": "GCA000209735_01718" }, { "type": "cds", "sequence": "contig_1", "start": 962547, "stop": 963740, "strand": "-", "frame": 3, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AYT1", "UniRef:UniRef90_C4XG58" ], "nt": "TTGAATAATACTCAAAATCAAAATGAAGATTTTGACGATTTATCCTTTGAAACAATTAATTTGGACAAAATGCTAGATGAAATCGGTGAGCTTAGAAATATTGAAAAAGGAGCTCATATTGAACTAAAAGGATTATCTAAAAAATATGAAGGTAATGAAAATTATACACTTGAAAATATTAATTTAGATATTCAACCAGGTAAATTTTGTATCTTTTTAGGACCTAGTGGTTGCGGTAAAACTACACTACTTAGAATGATTGCGGGATTAAATTCAATTACAAAAGGTGACTTATTATTCAATGGCAAAAGATATAATAACCTTTTACCTAGTGAAAGAAATATTGCAATGGTTTTTCAATCATATGCTTTATATCCTCACATGAACGTTTACAATAATATTGCTTTTGGTTTAAAAATAGCCAAAGAAAGAAAAGACGTTATTGACAAACGTGTTAAGGATGTTGCTAAAATTCTAAAAATAGAAGATTACTTATACAGAAAACCACGTGACTTATCTGGTGGTCAAAGACAACGTGTGGCAATAGGTCGTGCTATTGCTCGTAAACCTCAAGTGTTCTTGATGGATGAGCCTCTTTCTAACCTTGATGCAAAACTTCGTGAAAGTATGCGTCGTGAAATTGTTAATATCCACAGAATGCTTGGCGCAACCAGTATCTATGTAACTCATGACCAACTTGAAGCTATGACAATGGGTGATCAAATTGTTGTCTTCAATGATGGCTCAATTCAACAAAATGGTACTGGCCGTGACTTATACTTCAAACCTGCTAATGTCTTTGTAGCTAGATTTATTGGTTCTCCAACCATGAATGTTTTCAAAGCAGCATGCGAAAACGGCAAAATTGTTAGTGCTCAAAAAGACATTAAAATTAAAGCCCCAAAAGAATATTTGGAACAATTAAAAGACAAAAAATTAAAAATTGGTTTTAGAAGTGAAGACATCAAAATTCATGCTTCTGAAGTATCAAATTCAATTCATGCAACTATTAAAAATATTGAATTAATTGGTAAAGAACAACTTGTAACTGTTAAATTGGATAATGACGATATCGAACTTACAATTACAGCAAGCAATTCAGATACTTACGAGCTTTATTCAAAAGTTTACATTGAATTTTATGAACCTAGAATCCATATCTTCGATGCGGAAACAACAAAACGGATTAATTAA", "aa": "MNNTQNQNEDFDDLSFETINLDKMLDEIGELRNIEKGAHIELKGLSKKYEGNENYTLENINLDIQPGKFCIFLGPSGCGKTTLLRMIAGLNSITKGDLLFNGKRYNNLLPSERNIAMVFQSYALYPHMNVYNNIAFGLKIAKERKDVIDKRVKDVAKILKIEDYLYRKPRDLSGGQRQRVAIGRAIARKPQVFLMDEPLSNLDAKLRESMRREIVNIHRMLGATSIYVTHDQLEAMTMGDQIVVFNDGSIQQNGTGRDLYFKPANVFVARFIGSPTMNVFKAACENGKIVSAQKDIKIKAPKEYLEQLKDKKLKIGFRSEDIKIHASEVSNSIHATIKNIELIGKEQLVTVKLDNDDIELTITASNSDTYELYSKVYIEFYEPRIHIFDAETTKRIN", "aa_hexdigest": "6e4938bc3c00c1deea62cc35414349c4", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XG58", "query_cov": 1.0, "subject_cov": 0.9778325123152709, "identity": 0.997, "score": 772.0, "evalue": 1.16e-281, "valid": true, "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_A0A449AYT1" }, "pscc": { "uniref50_id": "UniRef50_A0A449AYT1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AYT1" ], "product": "ABC-type maltose/maltodextrin transporter ATP-binding protein MalK" }, "genes": [], "id": "OAIKOHHJFB_1721", "locus": "GCA000209735_01719" }, { "type": "cds", "sequence": "contig_1", "start": 964068, "stop": 964373, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATCTCAACCATTTGTGTTGCTTTGAATGCTTATGCTTATTCAAGATTTAAATTCGCTGGTTCAAAACACTCATTAACTATTATTATGATGTTGCAAATGATTCCTGCCACTTCATCTCTTATTTGTCTTTACATTATTGTGCGGATGGGGAATGATTTAGGAATTAATTCAATATTAATGCTGGTTATTATTTATTCTGGTGGAGCTATATCTTCAAATACCTTTATGCTAAAAAGTTATTTAGATACAGTTTCAAGAGAATTAGATGACTCAGCTAGAGTTGATGGATGTTCTAACTGA", "aa": "MVISTICVALNAYAYSRFKFAGSKHSLTIIMMLQMIPATSSLICLYIIVRMGNDLGINSILMLVIIYSGGAISSNTFMLKSYLDTVSRELDDSARVDGCSN", "aa_hexdigest": "dd98732cdd46758f0f01f06dcbe70765", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11024.933999999987, "isoelectric_point": 7.704548072814942 }, "id": "OAIKOHHJFB_1722", "locus": "GCA000209735_01720" }, { "type": "cds", "sequence": "contig_1", "start": 964590, "stop": 964721, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAGTGAAAACAAAATTTATGATTCAATTGTTGATAACATTTCAACTAAAAAAGTTATCAACAAAAAAGTTCGTATCAACTATAACTTATCAGATACTAAACCTCCAACACCAATGGAAATAGTTTGA", "aa": "MNSENKIYDSIVDNISTKKVINKKVRINYNLSDTKPPTPMEIV", "aa_hexdigest": "6aaebf92ca75576726d99dd6b9b01785", "start_type": "ATG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4951.672700000002, "isoelectric_point": 9.15762119293213 }, "id": "OAIKOHHJFB_1723", "locus": "GCA000209735_01721" }, { "type": "cds", "sequence": "contig_1", "start": 964724, "stop": 965119, "strand": "-", "frame": 1, "gene": null, "product": "ABC transporter permease subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF2AB6" ], "nt": "ATGTTAGTTACTGGTAACTTACAAGCAATTCCAAAAGATATTTATGAAGCTGCTTCTATTGATGGAGCCAAAAATTATCATAAATTCTTTAAGCTAACACTACCTTCTTTATTATTAGCAATTGCCCCAATGTTAATTGGTCAATTTGTTGGAGCATTCAACAACTTCACAACTATTAGTATCTTTAGTGGCGGAGGCCCTGCGTTTGCTAAACCAACTGCTTATCAAGAAGGTGCAACTGATATTCTAATTTCATTTATCTATAAACTAGCCACTGGTGCAGTAAAAATTGAATCTGATCAAGCTTTTGCAGCAGCTTTGACAACTCTTGCAGCTCTATTAAGTATCGCTGTAGCAGCTAGAGGATTCATTAAATCTATGACAAGGAGGGACTAA", "aa": "MLVTGNLQAIPKDIYEAASIDGAKNYHKFFKLTLPSLLLAIAPMLIGQFVGAFNNFTTISIFSGGGPAFAKPTAYQEGATDILISFIYKLATGAVKIESDQAFAAALTTLAALLSIAVAARGFIKSMTRRD", "aa_hexdigest": "7bf93e1623a5a2fd25a7600fba67b3ab", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021AF2AB6", "query_cov": 0.916030534351145, "subject_cov": 0.8633093525179856, "identity": 0.725, "score": 169.0, "evalue": 5.23e-52, "valid": false, "product": "ABC transporter permease subunit", "uniref50_id": "UniRef50_UPI0021AF2AB6" }, "pscc": { "uniref50_id": "UniRef50_UPI0021AF2AB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF2AB6" ], "product": "ABC transporter permease subunit" }, "genes": [], "id": "OAIKOHHJFB_1724", "locus": "GCA000209735_01722" }, { "type": "cds", "sequence": "contig_1", "start": 965684, "stop": 966004, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATATTCTTCAAACAAAAAACTAAAGGGATTCTTTTACTTTTAGTAACCACATTATTGTATGCAATTTTTATTCCTTTTAGTTTCGGAGCTTATACTACTGGTATTGATGGTATGGAAGGGATTCTGAGTTTCATTGACTTAGGAGCTCGACACTTCAATTCTAGCATGGGCATATTTAGAGATGCTCGGCGTTACCTTTTTGGTGGTGTCATATCTGTTATTATCTTAACAATAGTATTAATTTACTTTACAGTTTGTTCAATTATTGCATTTAGAACAGCTAAATTAATGGAAGAAGGGTCACGTCCAAGTAAATGA", "aa": "MIFFKQKTKGILLLLVTTLLYAIFIPFSFGAYTTGIDGMEGILSFIDLGARHFNSSMGIFRDARRYLFGGVISVIILTIVLIYFTVCSIIAFRTAKLMEEGSRPSK", "aa_hexdigest": "85875a450262fe94b1b9272598bd0912", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11855.068399999987, "isoelectric_point": 9.726554679870606 }, "id": "OAIKOHHJFB_1725", "locus": "GCA000209735_01723" }, { "type": "cds", "sequence": "contig_1", "start": 966155, "stop": 966619, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002114FE86", "UniRef:UniRef90_UPI002114FE86" ], "nt": "TTGCTTAAATCTGAACTTTATTTCAAAGAATCGGCAATTTATGAAAAATATTCTTATGAAGGAAATTATAAAAAAGAATTAGCTTTAGCCTTAAAAGAAAGAGCAATAAATGCTGAGCAAGTAAGACTAAAATTCTTATATGAAAAAGTTTTAGCTATTTATGAAACAAAACTAAATTCTTTAAATCTTAGCTCAAATGAAAATTCTAACATTTTAAAAGAACAAGTTAGAAACAAAGAACAATATCAAAGCGAAAAAGAATTAGTTTCTAACAAGAAAAAACAACTTTATAATCAATATCTTGAAACAGTTAAGCAAACAGCTTTAAGATATAAAAATAAAGAAATATCAAAACAAGCTTTTAAACATAGTAAAATGGAAGCAAAGATTGATTATAATGAAAAAAGATATGAATTAAAACTTCAAACTAACAGTCTTAAAAATAAAGAAATTTTATCAACTTGA", "aa": "MLKSELYFKESAIYEKYSYEGNYKKELALALKERAINAEQVRLKFLYEKVLAIYETKLNSLNLSSNENSNILKEQVRNKEQYQSEKELVSNKKKQLYNQYLETVKQTALRYKNKEISKQAFKHSKMEAKIDYNEKRYELKLQTNSLKNKEILST", "aa_hexdigest": "5f33af9db1673729df1141a3ff681cf8", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 965852, "stop": 966619, "observations": { "mutation": [ 966049, 966157 ], "stop": [ 966049, 966157 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002114FE86", "query_cov": 1.0, "subject_cov": 0.4, "identity": 0.948, "score": 263.0, "evalue": 2.59e-85, "gene_start": 1, "gene_end": 154, "reference_start": 1, "reference_end": 154, "reference_sequence": "MLKSELYFKESSIYEKYSYEGNYKKELALALKERAINAEQVRLKFLYEKALAIYETKLNSLNLSSDENPNILKEQVRNKKQYQSEKELVSNKKKELYNQYLETVKQTALRYKNKEISRQAFKHSKMEAKIDYNEKRYELKLQTNSLKNKEILSSWFFRRQAEMRVVSKIYESKVNEAVKTVPIECTRNIKWLAAIISFIFPGLSELIFFKQKAKGIFLLIVTTLLYAIFIPFSFGAYTTGTDGMEGILSFIDLGARHFNSSMGIFRDARRYLFGGVISVIILTIVLIYFIVCSIIAFRTAKLMEEGSRPSKWSYTKRWLNTSGFPWMISITGWILMLFIVLAPIITSVLISFTDYGYMHQAPTQPVHWVVWNNEDFDEFIVIMEF" }, "paralog": false, "identity": 0.9372549019607843, "score": 463.0, "evalue": 2.68e-166, "description": "Internal stop codon at 966049, 966157. Nonsense mutation around 966049, 966157.", "psc": { "uniref90_id": "UniRef90_UPI002114FE86", "uniref50_id": "UniRef50_UPI002114FE86", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002114FE86", "UniRef:UniRef50_UPI002114FE86" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002114FE86", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002114FE86" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 18426.88150000001, "isoelectric_point": 9.476417350769044 }, "id": "OAIKOHHJFB_1726", "locus": "GCA000209735_01724" }, { "type": "cds", "sequence": "contig_1", "start": 966620, "stop": 967696, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8K8F0", "UniRef:UniRef50_UPI002113FE78", "UniRef:UniRef90_A0A3P8K8F0" ], "nt": "ATGAAGATTAACAAAAATTTATTTTTACGAGCTAGAACAAAACTACAAGATAACTTAAAAAGAGAACTTTCAAGTTTATATGCGGCTTATTCAAATAAAATTAAAGCTATTAAAGATGCTATTAAAAAGATATCGTTTGCTGATAACGAAATCTCAATGATTAAGTATGAAATTAAAGCGTTAATAAAAGAAAAAAAGGCTCTTAAAAAATATGTACTAGAATTTCAAAAATCACTAAGACTAACAGCTGACATTGATGAAAAGAAGAATGAATTATTAGAAGAATTAAAGCAAAAAACAATTAAAGAAGAAAATAAAATTCTTTCTAAATATGCTTTATTCAATATAACTCTAAAATATCTTAAACATAATTCAGATCTTGATTTTGACATCAATAAAATTAAAAACTATTTGCATAAACAAGAACTAAAAATATTAAATACTTTAGAAGACCCAAAATCTTATTTCCAAAACTTTTATCAAAAATTGGAAAAACGTCGTTTAGAATTAATTGAAAAACGTAATTCACTAAACCACAAATATCAAAATAATAAATTTATTGAATTAAAGATTTATAAAGCAAACAAATACAACATTAAATTAGAAACAAATCAAAAAAATCTTGCTTTAGAATATGAATATAATCAAAAAGCAGAATTACAAAAACAAGAAGTAAAAGCATATAAAAAAGAAGCATATGCTAAAATCGAAGAACACAAAAATGAGATTAAACGTGTTGAAAAAGGCAATATTGAAAAAATTAAAAAAATAAAGCAAAATGGAAAATCAAAAATTAAGATTGTAAATCAAAATTTCAAGCAACAACTAAAGAAAATCGATGATTTAGTTGCTACTAGAAATTACCAACAATATTTAGAATTTTTAGCTAAAAATAACTTTATAGATTCTAATAAAGATGAATCTATAAAAATTACCAAAAAATCTGTTTTACAGTCATTTAAGAAAAATAAACAACTTGTCTATAATGACAAAAAAACATCTGCTTTAGCTAAAATATTTAAAAAATTATTGTTTGGTTTTTTTAACACAAAAAGCCTAAAAAAAGAATTTGAATGA", "aa": "MKINKNLFLRARTKLQDNLKRELSSLYAAYSNKIKAIKDAIKKISFADNEISMIKYEIKALIKEKKALKKYVLEFQKSLRLTADIDEKKNELLEELKQKTIKEENKILSKYALFNITLKYLKHNSDLDFDINKIKNYLHKQELKILNTLEDPKSYFQNFYQKLEKRRLELIEKRNSLNHKYQNNKFIELKIYKANKYNIKLETNQKNLALEYEYNQKAELQKQEVKAYKKEAYAKIEEHKNEIKRVEKGNIEKIKKIKQNGKSKIKIVNQNFKQQLKKIDDLVATRNYQQYLEFLAKNNFIDSNKDESIKITKKSVLQSFKKNKQLVYNDKKTSALAKIFKKLLFGFFNTKSLKKEFE", "aa_hexdigest": "698386a4707e07e5c43ee6f6916fd88a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002113FE78", "query_cov": 0.8715083798882681, "subject_cov": 0.8167539267015707, "identity": 0.84, "score": 416.0, "evalue": 4.28e-142, "valid": false, "uniref50_id": "UniRef50_UPI002113FE78" }, "pscc": { "uniref50_id": "UniRef50_UPI002113FE78", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002113FE78" ] }, "genes": [], "truncated": "both", "pseudogene": { "start": 966317, "stop": 967834, "observations": { "insertion": [ 967579 ], "deletion": [ 967497 ], "mutation": [ 966621, 967810 ], "stop": [ 966621, 967512, 967515, 967539, 967810 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8K8F0", "query_cov": 0.9972067039106145, "subject_cov": 0.4518987341772152, "identity": 0.852, "score": 491.0, "evalue": 8.54e-166, "gene_start": 2, "gene_end": 358, "reference_start": 48, "reference_end": 404, "reference_sequence": "MEKLKLFNWYGEEFDTILPEEQDTLKAYKHHVRNVVNRRIDKINSQKKINKNLFLRARTKLQDNLKRELSSLYASYSNKIKAIKDAIKKISFANSTISLIKYEIKALIKEKKALKKYVLEFQKSLRLTADTDEKKTELLEELKQKTIKEENEILSKYALFNITLKYLKHNPDLDFDIDKIKNHLHEQELKVLNTLEDPKSYFQNFYQKLENRRLKLIEKRNSLNHKYQNNKSIELKIYKANKYNIKLETNQKILALEYKYNHKAELQKQEVKAYKKEAYAKIEEHKNKIKRVEKDNIEKIKKIKQNGNSKIKIINQNFRQQLKKIDDLVATRNYQQYLEFLAKNNFINSNIEESKKITKKSVLQSFKKSGQLVYNDKKTSALAKIFKKLFFGFFNTKSLKKEFEWLLKSELYFKESSIYEKYSYEGNYKKELALALKERAINAEQVRLKFLYEKALAIYETKLNSLNLSSDENPNILKEQVRNKKQYQSEKELVSNKKKELYNQYLETVKQTALRYKNKEISRQAFKHSKMEAKIDYNEKRYELKLQTNSLKNKEILSSWFFRRQAEMRVVSKIYESKVNEAVKTVPIECTRNIKWLAAIISFIFPGLSELIFFKQKAKGIFLLIVTTLLYAIFIPFSFGAYTTGTDGMEGILSFIDLGARHFNSSMGIFRDARRYLFGGVISVIILTIVLIYFIVCSIIAFRTAKLMEEGSRPSKWSYTKRWLNTSGFPWMISITGWILMLFIVLAPIITSVLISFTDYGYMHQAPTQPVHWVVWNNEDFDEFIVIMEF" }, "paralog": false, "identity": 0.8831683168316832, "score": 794.0, "evalue": 5.09e-287, "description": "Internal stop codon at 966621, 967512, 967515, 967539, 967810. Frameshift due to insertion around 967579. Frameshift due to deletion around 967497. Nonsense mutation around 966621, 967810.", "psc": { "uniref90_id": "UniRef90_A0A3P8K8F0", "uniref50_id": "UniRef50_A0A3P8K8F0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8K8F0", "UniRef:UniRef50_A0A3P8K8F0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8K8F0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8K8F0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 42883.55039999999, "isoelectric_point": 9.822805976867674 }, "id": "OAIKOHHJFB_1727", "locus": "GCA000209735_01725" }, { "type": "cds", "sequence": "contig_1", "start": 968239, "stop": 968343, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCAAAATCCATACATTAAACCCAAATTAAATATAGACAAATTTAGTTTTTACTTTTTAGTTTCTTGTTTAGCAGCAATTACAGTCTTAATGATTTTTTGA", "aa": "MDQNPYIKPKLNIDKFSFYFLVSCLAAITVLMIF", "aa_hexdigest": "a0d5561bfb9bc7bbbb48c77e6151620b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3984.785399999999, "isoelectric_point": 7.898231315612794 }, "id": "OAIKOHHJFB_1728", "locus": "GCA000209735_01726" }, { "type": "cds", "sequence": "contig_1", "start": 968343, "stop": 968432, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGAAAAATTAACAGCAATTTACAAAGAAAAAATTGACACAATTAATAGTTCAACTGAATCGACAGCTGTTATTAGATTTGAATAA", "aa": "MYEKLTAIYKEKIDTINSSTESTAVIRFE", "aa_hexdigest": "0dc7400cd9bf7eca0ac9adff960cfd65", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3381.8024000000005, "isoelectric_point": 5.155035209655761 }, "id": "OAIKOHHJFB_1729", "locus": "GCA000209735_01727" }, { "type": "cds", "sequence": "contig_1", "start": 968523, "stop": 968903, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGATTTAATCAATGCAAAAATTGCTGAAGCTAAAAAAGTTTCACAACTTTTATCTAAAAAAGCAGTAGCTGATGAAGTTATTACAAAAGCAGTTAAAGCATATCTTTACGCAAGATACATTAACCAAGCTGAAGTTAGAAATCTTGGTGACAAATTAACTAAAGAATTTCAATTTCCAGCAAAAGATAAAAGCACTAAAGTGCCAGCTTCTAAAGTTATTGAAATTGTTGAACAATATAGAAAAAATCAAACAGTTGACTTAATTTATCAAACATTATCTTCAACTAAATCATTAGCTGATGGTGGATTGAATGTAATTCAATACCAACCAGGACGTATTGACCAATCAAATCCTCAATTTGGTAATGTTGCATGA", "aa": "MQDLINAKIAEAKKVSQLLSKKAVADEVITKAVKAYLYARYINQAEVRNLGDKLTKEFQFPAKDKSTKVPASKVIEIVEQYRKNQTVDLIYQTLSSTKSLADGGLNVIQYQPGRIDQSNPQFGNVA", "aa_hexdigest": "040187f514c64e9f0fa1172cff937f15", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14046.941599999984, "isoelectric_point": 9.49382381439209 }, "id": "OAIKOHHJFB_1730", "locus": "GCA000209735_01728" }, { "type": "cds", "sequence": "contig_1", "start": 969090, "stop": 969275, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATTCAGCTAACAATTTACCAGTACATGCTGTGTTAAACAGTACTTTAGAATCTAAATTAGGCATGAATTTCAAAGATATTCGTGATAAATATACTGAAAAAGGTACTGGATTACGTAATGCATTAGCAGCAATTTTAGGTTTGGAAAAACTTTCGGATTTACAAGGTAATGGCCAAACTTGA", "aa": "MYSANNLPVHAVLNSTLESKLGMNFKDIRDKYTEKGTGLRNALAAILGLEKLSDLQGNGQT", "aa_hexdigest": "9e1b85fcaf7c5daa9a61b449701ccc49", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6621.468400000001, "isoelectric_point": 8.08609218597412 }, "id": "OAIKOHHJFB_1731", "locus": "GCA000209735_01729" }, { "type": "cds", "sequence": "contig_1", "start": 969315, "stop": 969428, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCAAATTGAAATATACAGATGCTGCTGAATTTAATAGAGATTATGATTCATCAATTCAAGTAATTAATAATTTATCAAATGTAGGCGATTCTCCAAGTGCAAGTACTTGA", "aa": "MSKLKYTDAAEFNRDYDSSIQVINNLSNVGDSPSAST", "aa_hexdigest": "cd325d09b4557dbba019b2b12b6d5bc6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4039.3065000000006, "isoelectric_point": 4.359459877014161 }, "id": "OAIKOHHJFB_1732", "locus": "GCA000209735_01730" }, { "type": "cds", "sequence": "contig_1", "start": 969864, "stop": 970037, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGAAGAAATTACTTTTCTTAAAAAATGGAAAAATTTCAACAGGTCTTTACACAGAAAATCCTATCCATGTTGAATTCAAAAAAGCAGTTGATTTAATGTCAGAATTCTTCTTTCCTATATATGAAGCTGCATATATCCTAGACTCAGGAGCATATGCTACTTCAGTATGA", "aa": "MMKKLLFLKNGKISTGLYTENPIHVEFKKAVDLMSEFFFPIYEAAYILDSGAYATSV", "aa_hexdigest": "78f115e3329eec8aa4dee78466592bf1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6482.537899999999, "isoelectric_point": 6.534346961975097 }, "id": "OAIKOHHJFB_1733", "locus": "GCA000209735_01731" }, { "type": "cds", "sequence": "contig_1", "start": 970089, "stop": 970475, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32F26", "UniRef:UniRef90_UPI0001E32F26" ], "nt": "ATGCCTACAGGGGCAACAGATGAAAAAATTGTGCCAGATATTTTTTATGCACCACATGATAGAATTACAACTTTAGTTGCATCTAATGCAGTTGCTGATTTAGATCAATTTTCACCAGGATTATTAGATCGTATTGCTAAAAAAGTTAAAGCAACTGAAGAAGAAAAGAAACAACTTAGATCATTTGGTTCAGTAGTCGGTATTGAAAAAATTACTCAATTACCAGCTGAAAAACTTTTTGGTATTAGACACAACACTGAAGGTGTTATAGTTGCTTCAACAAAATCTTTAGCAGATGTTAAAAAAGACTTAGCTAAACCTGAAAATGATACATTGCTAAAATTAGTAAAACAAGGTCGTGCATTTTTAAGATTCCAAGACTTCTGA", "aa": "MPTGATDEKIVPDIFYAPHDRITTLVASNAVADLDQFSPGLLDRIAKKVKATEEEKKQLRSFGSVVGIEKITQLPAEKLFGIRHNTEGVIVASTKSLADVKKDLAKPENDTLLKLVKQGRAFLRFQDF", "aa_hexdigest": "e3df7699676ab7edcd1fc9399818730e", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "both", "pseudogene": { "start": 969833, "stop": 970718, "observations": { "insertion": [ 969890 ], "mutation": [ 970091, 970490 ], "stop": [ 970091, 970490 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001E32F26", "query_cov": 1.0, "subject_cov": 0.43537414965986393, "identity": 1.0, "score": 248.0, "evalue": 8.98e-81, "gene_start": 1, "gene_end": 128, "reference_start": 82, "reference_end": 209, "reference_sequence": "MSNFKKTFKKIAIGFATLAAPVTALATISCGTTESKTILIAVDGVQQKFYDKAIELFKKTKSGQNGYEIKTIAKDVWGALEMPTGATDEKIVPDIFYAPHDRITTLVASNAVADLDQFSPGLLDRIAKKVKATEEEKKQLRSFGSVVGIEKITQLPAEKLFGIRHNTEGVIVASTKSLADVKKDLAKPENDTLLKLVKQGRAFLRFQDFWYGNGILGAVAHEYKAEDMMKKLLFLKNGKISTGLYTENPIHVEFKKAVDLMSEFFFPIYEAAYILDQEHMLLQYEAKEILNKMT" }, "paralog": false, "identity": 0.9931972789115646, "score": 560.0, "evalue": 1.61e-206, "description": "Internal stop codon at 970091, 970490. Frameshift due to insertion around 969890. Nonsense mutation around 970091, 970490.", "psc": { "uniref90_id": "UniRef90_UPI0001E32F26", "uniref50_id": "UniRef50_UPI0001E32F26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001E32F26", "UniRef:UniRef50_UPI0001E32F26" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001E32F26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001E32F26" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 14150.149499999978, "isoelectric_point": 8.859583854675293 }, "id": "OAIKOHHJFB_1734", "locus": "GCA000209735_01732" }, { "type": "cds", "sequence": "contig_1", "start": 970488, "stop": 970718, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAATTTTAAGAAAACTTTTAAAAAAATAGCAATTGGCTTTGCAACCTTAGCAGCTCCAGTGACAGCATTAGCTACAATTAGTTGTGGTACTACTGAAAGCAAAACAATTTTAATTGCAGTTGATGGCGTTCAACAAAAATTCTATGACAAAGCAATTGAATTGTTTAAGAAAACAAAATCAGGCCAAAATGGCTATGAAATTAAAACAATAGCTAAAGATGTTTGA", "aa": "MSNFKKTFKKIAIGFATLAAPVTALATISCGTTESKTILIAVDGVQQKFYDKAIELFKKTKSGQNGYEIKTIAKDV", "aa_hexdigest": "05fdf836fc0fafb13ac8783bb023d959", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8232.566499999997, "isoelectric_point": 9.630819129943848 }, "id": "OAIKOHHJFB_1735", "locus": "GCA000209735_01733" }, { "type": "cds", "sequence": "contig_1", "start": 971562, "stop": 971837, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG64", "UniRef:UniRef90_C4XG64" ], "nt": "TTGAATTTATCCAAGAATGAGAATTCGATTGAACAAGCTCATAAGAATAAAAAATCTAATTTTAATTTAGCCAAAAAAATAATTAATTATCGTAAAGAAAATCCAAAAATGTTTGCTGAAAAAGAAAATACTTATGCTAATTTAGATGATCTCTTTAAACCCGGTTATGATTATAAAGGACTTGGATATAAATTAGTTAAAGCAAAGCAAAAAGATAAAAAGTTTTTAGTTTTAGTCAATTCAATTTATTATCATACAGCTTATGGTCAAGAATGA", "aa": "MNLSKNENSIEQAHKNKKSNFNLAKKIINYRKENPKMFAEKENTYANLDDLFKPGYDYKGLGYKLVKAKQKDKKFLVLVNSIYYHTAYGQE", "aa_hexdigest": "65d3d91ba46794b1bd58859ead91a0e1", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 971436, "stop": 971858, "observations": { "mutation": [ 971564 ], "stop": [ 971564 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG64", "query_cov": 1.0, "subject_cov": 0.65, "identity": 0.9890000000000001, "score": 180.0, "evalue": 8.23e-57, "gene_start": 1, "gene_end": 91, "reference_start": 8, "reference_end": 98, "reference_sequence": "METSHIFLNLSKNENSIEQAHKNKKSNFNLAKKIINYRKENPKMFAEKENTYANLDDLFKPGYDYKGLGYKLVKAKQKDKKFLVLVNSIYYHTAYGQEWNLVFKNAPTKINVIFSNNSSINNEKITFSGNTGLMLVELTY" }, "paralog": false, "identity": 0.9857142857142858, "score": 279.0, "evalue": 3.04e-98, "description": "Internal stop codon at 971564. Nonsense mutation around 971564.", "psc": { "uniref90_id": "UniRef90_C4XG64", "uniref50_id": "UniRef50_C4XG64", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG64", "UniRef:UniRef50_C4XG64" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG64", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG64" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10713.135499999997, "isoelectric_point": 9.589752769470213 }, "id": "OAIKOHHJFB_1736", "locus": "GCA000209735_01734" }, { "type": "cds", "sequence": "contig_1", "start": 971903, "stop": 972097, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTTTGGAGTTAAAACTTTTTATCTCGAAATTAAGCAAAATGATTTTAAAGTTTTAAATGTAATGAGTTATGGATTAAGTAACCTATTAATTCAAAGTGGCAATCCAATTCTTTATAATGGTAATGAATTAAATATGAGAGGTGGCCCACAAGCACATTCTGATGCCTTTTTAAGAGAAGCTTTTAAATGA", "aa": "MIFGVKTFYLEIKQNDFKVLNVMSYGLSNLLIQSGNPILYNGNELNMRGGPQAHSDAFLREAFK", "aa_hexdigest": "02683061485069b064bc45f3d1003325", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7254.2836, "isoelectric_point": 8.085898780822756 }, "id": "OAIKOHHJFB_1737", "locus": "GCA000209735_01735" }, { "type": "cds", "sequence": "contig_1", "start": 972353, "stop": 972502, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTCAGAAAATAAACATGAATTTAAAGACGCAACAGGCTTAGAAAAGAGAAAATTATTTAATGAGCAAAGATTAGCCTTAAGAGAAGTAATAAAAGAAACTAAAGATCAAAGATCAAGCGATGATATATTTTTCTTTGGTGAATGA", "aa": "MNSENKHEFKDATGLEKRKLFNEQRLALREVIKETKDQRSSDDIFFFGE", "aa_hexdigest": "58a91c581e03181e6b7760ec7eedf752", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5876.4847, "isoelectric_point": 5.796065330505371 }, "id": "OAIKOHHJFB_1738", "locus": "GCA000209735_01736" }, { "type": "cds", "sequence": "contig_1", "start": 972830, "stop": 973168, "strand": "-", "frame": 1, "gene": null, "product": "alpha-amylase family glycosyl hydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF384D3" ], "nt": "TTGTCTTATGAAGGTGAATTTAAAAATGCAAAATTTATTGTGCCTTTTAACAAATCTATTAAGCCTTCAAATGTTATGTATCAATTAACAATTTATTCTTTTGCAGATCGCAATAATGATGGTATTGGCGACTTTATTGGACTAAATGAAAATTTAGAATATTTTAGCAATTTAGGAATTGATACTTTATATCTTTCACCAATACATCCAGCCAGCTCATATCATGGCTATGATGTAATTGATTATACAGATGTTGCTCCTGAATTAGGTGGCATGGAAGCCTTTGATCAATTTTTAATAAATGCCCATAAAAAAGGCATAAAAGTTATTATGGATTGA", "aa": "MSYEGEFKNAKFIVPFNKSIKPSNVMYQLTIYSFADRNNDGIGDFIGLNENLEYFSNLGIDTLYLSPIHPASSYHGYDVIDYTDVAPELGGMEAFDQFLINAHKKGIKVIMD", "aa_hexdigest": "8c25356b7fcbd7a1eddd4f153d1f9748", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FF384D3", "query_cov": 0.9285714285714286, "subject_cov": 0.832, "identity": 0.769, "score": 169.0, "evalue": 1.57e-52, "valid": false, "product": "alpha-amylase family glycosyl hydrolase", "uniref50_id": "UniRef50_UPI002FF384D3" }, "pscc": { "uniref50_id": "UniRef50_UPI002FF384D3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF384D3" ], "product": "alpha-amylase family glycosyl hydrolase" }, "genes": [], "id": "OAIKOHHJFB_1739", "locus": "GCA000209735_01737" }, { "type": "cds", "sequence": "contig_1", "start": 973210, "stop": 973311, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59D51", "UniRef:UniRef100_A0AB32XCK3", "UniRef:UniRef50_A0AB32XCK3", "UniRef:UniRef90_A0AB32XCK3" ], "nt": "ATGAGTAAAAAAACAAGAAAAAAATTAGCATATTTTTCTTTAATTGCACCTATCGCTTTATGTTTAAGTGCAAGTTGCACAAGTGTTAAAAAAGATTATTAA", "aa": "MSKKTRKKLAYFSLIAPIALCLSASCTSVKKDY", "aa_hexdigest": "0d0991061a8fb19f63031ca6f39809b5", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI0001F59D51", "uniref100_id": "UniRef100_A0AB32XCK3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F59D51", "UniRef:UniRef100_A0AB32XCK3" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XCK3", "uniref90_id": "UniRef90_A0AB32XCK3" }, "psc": { "uniref90_id": "UniRef90_A0AB32XCK3", "uniref50_id": "UniRef50_A0AB32XCK3" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XCK3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XCK3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3666.4218999999994, "isoelectric_point": 9.835119438171386 }, "id": "OAIKOHHJFB_1740", "locus": "GCA000209735_01738" }, { "type": "cds", "sequence": "contig_1", "start": 974476, "stop": 975033, "strand": "-", "frame": 2, "gene": null, "product": "ECF transporter S component", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LHP5", "UniRef:UniRef90_C4XG67" ], "nt": "ATGCAAAAAAATGAGAATTTTACCAATAATATTGCAGCCAATGAAGATGACAAATCTTTTAATGAATTATCATTTGGCAAAAAGTTTAAGCAACACTTCGCTAAGTCATTTCGTCTTAGTGTTTTAGATATTGCTTTAGCAGGTATATTATTGGCAATTCATTTTGTTGTTGCAATAATTTCTAAGTTTACAATTTTAAAAATAATACCCTTAGAAACTGATGCAATTTTTTTTATATTATATGGAATCTTTTTTGGACCACTTAAAGGATCTTTAATATCATTTTTAGCTGATACTTTTATGATGCTTTTAACTGGTACAATTGGAACTTGGTATTATCTTTATGCATTGATTCCAATAGGCATTTGTATTTTAAGCTCCTTTTATTACTTTTTTTATCGTGCTAGTTTTTTATTTAGAATTATTTTTCCAATAATTGTAATTTCAGTTGGTTTCGCTTTATTACTCTATGTTGTTATAAATAGTGGAAATGATAAAGGTTATCATATTAAAGGAATTAAGAAGCTAAAATATTTACCTAAAAATGCAGTTTTATGA", "aa": "MQKNENFTNNIAANEDDKSFNELSFGKKFKQHFAKSFRLSVLDIALAGILLAIHFVVAIISKFTILKIIPLETDAIFFILYGIFFGPLKGSLISFLADTFMMLLTGTIGTWYYLYALIPIGICILSSFYYFFYRASFLFRIIFPIIVISVGFALLLYVVINSGNDKGYHIKGIKKLKYLPKNAVL", "aa_hexdigest": "a94329f6d0f86e7ade84262bce6e0fec", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 974173, "stop": 975045, "observations": { "mutation": [ 974325, 974382, 974478 ], "stop": [ 974325, 974382, 974478 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG67", "query_cov": 1.0, "subject_cov": 0.589171974522293, "identity": 1.0, "score": 343.0, "evalue": 2.58e-117, "gene_start": 1, "gene_end": 185, "reference_start": 5, "reference_end": 189, "reference_sequence": "MIQNMQKNENFTNNIAANEDDKSFNELSFGKKFKQHFAKSFRLSVLDIALAGILLAIHFVVAIISKFTILKIIPLETDAIFFILYGIFFGPLKGSLISFLADTFMMLLTGTIGTWYYLYALIPIGICILSSFYYFFYRASFLFRIIFPIIVISVGFALLLYVVINSGNDKGYHIKGIKKLKYLPKNAVLWMLLIYFNFSVIAFIFIIFQLLFAKNKKVKKRWFDYLFVFSIIIFIMIIFRWLIGPYVYINYYNYLYAGRKSLNYTGEHYYLITIPILLKSLITIPIYSIIMNPIFSVAILLKEKFVEKNSKISY" }, "paralog": false, "identity": 0.9862068965517241, "score": 535.0, "evalue": 2.49e-195, "description": "Internal stop codon at 974325, 974382, 974478. Nonsense mutation around 974325, 974382, 974478.", "psc": { "uniref90_id": "UniRef90_C4XG67", "product": "ECF transporter S component", "uniref50_id": "UniRef50_A0A3P8LHP5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG67", "UniRef:UniRef50_A0A3P8LHP5" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8LHP5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8LHP5" ], "product": "G-protein coupled receptors family 1 profile domain-containing protein" } }, "id": "OAIKOHHJFB_1741", "locus": "GCA000209735_01739" }, { "type": "cds", "sequence": "contig_1", "start": 975046, "stop": 976125, "strand": "+", "frame": 1, "gene": null, "product": "Phosphomannomutase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG68", "UniRef:UniRef90_C4XG68" ], "nt": "ATGTTAAAAATTTTTATTTGTTATTATATTATATATATTATGAACAAAAAATTAGAATTTGGAACAGCTGGAATTAGAGGAATTTTAGGTTCTGGTGAAGACCATTTAAATGTAGCTCATGTTCAAAGAGTTATTCATGGTTTTGCTAAATATTTAAAAAACAAATATAGCCACATTAATCCAATTACTGTTGTTATTGGACGTGATAACCGTAGATTCTCAGCTACATTTGCAACCTTAAGTGCTCAAATTTTAAGCCAATATGACATTAAGGTTATTTTTCCTCTATACTTGACTCCAACACCTTTTGTTAGTTTTGCAATTTTAAGAGAACATGCTCAAGGTGGAATTAACATTACAGCAAGTCATAATCCAGCAGAATATAATGGAATAAAACTTTACAACGACATGGGAAGTCAATGCTTACCTGAAGAAATTAAAGAAATTAGTGCTTATTTTGAAGATTATTCAAAATATGATGAAAATCTTAAAGTTCCTAAAAAAGAAGCTATTTTTAAAGAACTGAAAAACCATAAGTTTTATCCAAAGAAACTTTACAATGAATATATCGATAGGATTCTTAAATTAGGAGGACTTGTTGACTTATCTGATATTTCGTTAATCTATTCTCCATTACACGGGACAGGACGTGATTTTGTATCTGATATATTTAATAAATTTATTGAAAGATACAGATTAAAACCTGAAAATGTTCATTATGTCAAAAGTCAAATGTATCCAAGTACATCTTTTAGACATTGCAAATATCCAAACCCTGAAAGAGAAAAAACATACAGCAAATCTATTAAACAAGCTCAAAAACATAACGCAGACATTATATTAGTAACTGATCCAGACAGCGATAGAGTCGGTTTAGTTGTTAGACATAATAACGAATATGTGAGATTAAATGGTAATGAAACAGCAACAATTATTTTTGATTACTTGATTAGTAAATCAAGTATAAGTAATTTTGCAAATCAATACATGGTTTACTCATATGTTTCATCAAATATGCCAGCAATTTTAGCTAGAAAAAATGGTATAAAAGTTTATGAAGTTCCAACAGGATTTAAATGA", "aa": "MLKIFICYYIIYIMNKKLEFGTAGIRGILGSGEDHLNVAHVQRVIHGFAKYLKNKYSHINPITVVIGRDNRRFSATFATLSAQILSQYDIKVIFPLYLTPTPFVSFAILREHAQGGINITASHNPAEYNGIKLYNDMGSQCLPEEIKEISAYFEDYSKYDENLKVPKKEAIFKELKNHKFYPKKLYNEYIDRILKLGGLVDLSDISLIYSPLHGTGRDFVSDIFNKFIERYRLKPENVHYVKSQMYPSTSFRHCKYPNPEREKTYSKSIKQAQKHNADIILVTDPDSDRVGLVVRHNNEYVRLNGNETATIIFDYLISKSSISNFANQYMVYSYVSSNMPAILARKNGIKVYEVPTGFK", "aa_hexdigest": "336e5ae5457fab16cff26d42d6dfea7c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 974971, "stop": 976428, "observations": { "mutation": [ 976123 ], "stop": [ 976123 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG68", "query_cov": 1.0, "subject_cov": 0.6468468468468469, "identity": 1.0, "score": 719.0, "evalue": 4.59e-259, "gene_start": 1, "gene_end": 359, "reference_start": 26, "reference_end": 384, "reference_sequence": "MKRFVIFIGCNIIGKILIFLHILNYMLKIFICYYIIYIMNKKLEFGTAGIRGILGSGEDHLNVAHVQRVIHGFAKYLKNKYSHINPITVVIGRDNRRFSATFATLSAQILSQYDIKVIFPLYLTPTPFVSFAILREHAQGGINITASHNPAEYNGIKLYNDMGSQCLPEEIKEISAYFEDYSKYDENLKVPKKEAIFKELKNHKFYPKKLYNEYIDRILKLGGLVDLSDISLIYSPLHGTGRDFVSDIFNKFIERYRLKPENVHYVKSQMYPSTSFRHCKYPNPEREKTYSKSIKQAQKHNADIILVTDPDSDRVGLVVRHNNEYVRLNGNETATIIFDYLISKSSISNFANQYMVYSYVSSNMPAILARKNGIKVYEVPTGFKWIGMIINEEIKKDQHCMFAFEESYGSLIDESLARDKDAIQSVAILVKIAAEYKKENKTIVDALNEIYKENGYIVSGNVEIMTDENTSLSNIQNSFINLDLDNKIVDDFNKKTDFTKSNMIKITFSNDDSWLALRPSGTEPKIKFYIFAFGKDQQEAQAKFDNFARIIKEIK" }, "paralog": false, "identity": 0.9958762886597938, "score": 966.0, "evalue": 0.0, "description": "Internal stop codon at 976123. Nonsense mutation around 976123.", "psc": { "uniref90_id": "UniRef90_C4XG68", "product": "Phosphomannomutase", "uniref50_id": "UniRef50_C4XG68", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG68", "UniRef:UniRef50_C4XG68" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG68", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG68" ], "product": "Phosphomannomutase" } }, "id": "OAIKOHHJFB_1742", "locus": "GCA000209735_01740" }, { "type": "cds", "sequence": "contig_1", "start": 976132, "stop": 976512, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02880.22" ], "nt": "ATGATAATAAATGAAGAAATCAAAAAAGATCAACACTGCATGTTTGCTTTTGAAGAAAGCTATGGTTCATTAATTGATGAAAGTTTAGCTAGAGATAAAGATGCTATTCAATCAGTTGCTATTTTAGTTAAAATAGCTGCAGAATACAAAAAAGAAAACAAAACAATTGTTGATGCTTTAAATGAAATATATAAAGAAAATGGTTACATTGTTAGTGGTAATGTTGAAATTATGACTGATGAAAATACATCATTAAGTAATATTCAAAACAGTTTTATTAACTTAGATTTAGACAATAAAATTGTTGATGACTTTAACAAAAAAACTGATTTTACTAAATCAAATATGATTAAAATTACATTCTCAAATGACGATTCTTGA", "aa": "MIINEEIKKDQHCMFAFEESYGSLIDESLARDKDAIQSVAILVKIAAEYKKENKTIVDALNEIYKENGYIVSGNVEIMTDENTSLSNIQNSFINLDLDNKIVDDFNKKTDFTKSNMIKITFSNDDS", "aa_hexdigest": "1f68d738d7186442c746fb060d112ead", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02880.22", "name": "PGM_PMM_III", "length": 57, "aa_cov": 0.4523809523809524, "hmm_cov": 0.9649122807017544, "evalue": 1.086917634295132e-06, "score": 24.884675979614258, "start": 4, "stop": 60 } ], "seq_stats": { "molecular_weight": 14355.937999999982, "isoelectric_point": 4.472285270690917 }, "id": "OAIKOHHJFB_1743", "locus": "GCA000209735_01741" }, { "type": "cds", "sequence": "contig_1", "start": 976706, "stop": 976900, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTTTTAAAAAGTCCATTAATTGATTATTTTTCGTCGACATATTTAGTAATAAAGCTACCTGAAATTCCAATTACTGGTTTAACTGAAATTCATAATTTAGTATCTAAAGTTTTTAAATCACGAATAATGCTACGTGTTTCTAAAAGAAGCATTGTAGTTACTACACTATATGTTTTTAAGCCTGAGTAA", "aa": "MNFLKSPLIDYFSSTYLVIKLPEIPITGLTEIHNLVSKVFKSRIMLRVSKRSIVVTTLYVFKPE", "aa_hexdigest": "67dff05b1e79d9cedba223846d52d62f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7415.8406, "isoelectric_point": 9.89874973297119 }, "id": "OAIKOHHJFB_1744", "locus": "GCA000209735_01742" }, { "type": "cds", "sequence": "contig_1", "start": 976933, "stop": 977205, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTATAACATTCTTAATCATTTTTGGTTTTGCTCAACCACATCATAACTATGCAAAAATTTTAACAAATGGTGTTGGAACTCAAATTATTGAAGATTCAGGTAACAGAGTATTCTTCGGAATGAGAGAATTTACAACATTCTTCTATATATTGGTTGTTAACTTCTTAATTAACTTTATTTATCCTAAATATAAAAAAGTTGCAGTTGAAATTTCTTGTGGGCCTTCACCAGATTTAGTGTTAGCGTACTTTAAGAAAATTAACTATTGA", "aa": "MTITFLIIFGFAQPHHNYAKILTNGVGTQIIEDSGNRVFFGMREFTTFFYILVVNFLINFIYPKYKKVAVEISCGPSPDLVLAYFKKINY", "aa_hexdigest": "1ec7a4f6836d5d243dfd1b43fbe04920", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10396.10749999999, "isoelectric_point": 9.13157596588135 }, "id": "OAIKOHHJFB_1745", "locus": "GCA000209735_01743" }, { "type": "cds", "sequence": "contig_1", "start": 977455, "stop": 978006, "strand": "-", "frame": 2, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG69", "UniRef:UniRef90_C4XG69" ], "nt": "ATGAATAACAACGAAAAAGATACTACCAAACTAGAACAAGTGCATAACCTTGAAGTTAGTAAAGAAGCTATTCAAGAAGTTAAAGATATGGTTTTTGAAGCAAAAAATCAAATCCAAGAACCTAAAAAGGAAATTCTTGATGAAAAATCTCTTGACAGTCTTGAAATGGTCCGTCATAATTATGAAAAAGATAATCCTAAAAACAAGGAAGTTAGGAAAAGAATTGCTAAACTCTATGCTCGTCGTTTAGTTTTTATCTTTTTATCAGCATTGGTCTTTAACTTTGGTGTGCAAGTATTCTTAAATAGAGGTGAAACTATTCCTAGTGGACTTAGTGGAGCTCCCTTATTAATTAACTTAATTGCACCGAGTACTAAGCCTTATTTTGCTTTAATTTACTTAGCTTTTAACTTACCGCTTCTTTTAGGATTTGGATTTAAGATTAAGAAAAGTTTTACTTTGATGACACTAGCTTTTATGCTATTTCAAATATTAACTAATTTGATATTAACTTCAGTTCCAATTGGAGAAAATAAAATAGTTTTAGACTGA", "aa": "MNNNEKDTTKLEQVHNLEVSKEAIQEVKDMVFEAKNQIQEPKKEILDEKSLDSLEMVRHNYEKDNPKNKEVRKRIAKLYARRLVFIFLSALVFNFGVQVFLNRGETIPSGLSGAPLLINLIAPSTKPYFALIYLAFNLPLLLGFGFKIKKSFTLMTLAFMLFQILTNLILTSVPIGENKIVLD", "aa_hexdigest": "3c08ae7db96ca4ca29606584b3feb53f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 977152, "stop": 978015, "observations": { "mutation": [ 977310, 977373, 977424, 977457 ], "stop": [ 977310, 977373, 977424, 977457 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG69", "query_cov": 1.0, "subject_cov": 0.4295774647887324, "identity": 1.0, "score": 321.0, "evalue": 8.24e-107, "gene_start": 1, "gene_end": 183, "reference_start": 4, "reference_end": 186, "reference_sequence": "MNIMNNNEKDTTKLEQVHNLEVSKEAIQEVKDMVFEAKNQIQEPKKEILDEKSLDSLEMVRHNYEKDNPKNKEVRKRIAKLYARRLVFIFLSALVFNFGVQVFLNRGETIPSGLSGAPLLINLIAPSTKPYFALIYLAFNLPLLLGFGFKIKKSFTLMTLAFMLFQILTNLILTSVPIGENKIVLDWLHEHINFAPGWTKEIKVGSVTLENPNSWPIFAYGAIGSGCAGTAIAIAWKNGGSTGGTDIVAYYFSTKKQKSVGNILSLISIIMTITFLIIFGFAQPHHNYAKILTNGVGTQIIEDSGNRVFFGMREFTTFFYILVVNFLINFIYPKYKKVAVEISCGPSPDLVLAYFKKINYWHGYTVVEGISGYSGLKTYSVVTTMLLLETRSIIRDLKTLDTKLWISVKPVIGISGSFITKYVDEK" }, "paralog": false, "identity": 0.9825783972125436, "score": 527.0, "evalue": 2.06e-190, "description": "Internal stop codon at 977310, 977373, 977424, 977457. Nonsense mutation around 977310, 977373, 977424, 977457.", "psc": { "uniref90_id": "UniRef90_C4XG69", "product": "ABC transporter permease", "uniref50_id": "UniRef50_C4XG69", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG69", "UniRef:UniRef50_C4XG69" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG69", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG69" ], "product": "ABC transporter permease" } }, "id": "OAIKOHHJFB_1746", "locus": "GCA000209735_01744" }, { "type": "cds", "sequence": "contig_1", "start": 978151, "stop": 978450, "strand": "+", "frame": 1, "gene": "rplU", "product": "50S ribosomal protein L21", "db_xrefs": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02888", "RefSeq:WP_013354830.1", "SO:0001217", "UniParc:UPI0001A8C1DC", "UniRef:UniRef100_C4XG70", "UniRef:UniRef50_A9NF54", "UniRef:UniRef90_C4XG70" ], "nt": "ATGATTGCTATTATCGAAACTGGAGGCAAACAAATTTTAGTTAAAGAAGGCCAAACCATCTTTATTGAAAAAATTGAAGGTAAAGAAGGTTCAACCGTTGACTTTGACAAGGTTTTATTAATCGACAATAAGATTGGTAAACCATATCTTAAAGGAGCCAAAGTTACTGGAAAAATCCAAAAACAAGGCAAAGCAAAAAAAATAGTTGTATATCGTCACAACCCTAAATCAACTCATAAGAGAAAATTGGGACACCGTCAACCTTACACACGTGTAGAAATTACAAAAATTGTAGGATAG", "aa": "MIAIIETGGKQILVKEGQTIFIEKIEGKEGSTVDFDKVLLIDNKIGKPYLKGAKVTGKIQKQGKAKKIVVYRHNPKSTHKRKLGHRQPYTRVEITKIVG", "aa_hexdigest": "912f8497cc2d988986112d18fe7b9be6", "start_type": "ATG", "rbs_motif": "AGGAGG", "ups": { "uniparc_id": "UPI0001A8C1DC", "ncbi_nrp_id": "WP_013354830.1", "uniref100_id": "UniRef100_C4XG70", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C1DC", "RefSeq:WP_013354830.1", "UniRef:UniRef100_C4XG70" ] }, "ips": { "uniref100_id": "UniRef100_C4XG70", "uniref90_id": "UniRef90_C4XG70" }, "psc": { "uniref90_id": "UniRef90_C4XG70", "gene": "rplU", "product": "50S ribosomal protein L21", "uniref50_id": "UniRef50_A9NF54", "kegg_orthology_id": "K02888", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A9NF54", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A9NF54" ], "product": "Large ribosomal subunit protein bL21" }, "genes": [ "rplU" ], "id": "OAIKOHHJFB_1747", "locus": "GCA000209735_01745" }, { "type": "cds", "sequence": "contig_1", "start": 978460, "stop": 978858, "strand": "+", "frame": 1, "gene": "rpmA", "product": "50S ribosomal protein L27", "db_xrefs": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02899", "SO:0001217", "UniParc:UPI0001A8C1DD", "UniRef:UniRef100_C4XG71", "UniRef:UniRef50_A0A3P8MF53", "UniRef:UniRef90_C4XG71" ], "nt": "ATGGCACATACGAAAGCCGGTGGTTCAACCCGTAATGGTCGTGACTCTCGTGGTCAAAGACTTGGAATCAAATTAGGTGACGGACAATTTTGTACAGCTGGATCAATTATCTTTAGACAACGTGGTACAAAGATTTTCCCAGGTACAAATTGTGGACGTGGAAATGACGATACATTATTTGCTTTAATTACAGGATATGTAAAATTTGAAACAAGAAGAAATAGAACATATGCCTCAGTATATCCAGAAAGAGTTTCAAGTGCTCTTTCACAACCAGTTGTAAAAAAGGCTGCTGTTAAAAAAGAAACCGCTCCTAAAGTAGCGGTTAAAAAACCAGCTGCAAAAAAAGCAACTACAACAACTAAAAAAGTTAGTGCTGCAAAAAAAGCAACTAAATAA", "aa": "MAHTKAGGSTRNGRDSRGQRLGIKLGDGQFCTAGSIIFRQRGTKIFPGTNCGRGNDDTLFALITGYVKFETRRNRTYASVYPERVSSALSQPVVKKAAVKKETAPKVAVKKPAAKKATTTTKKVSAAKKATK", "aa_hexdigest": "9de227b38602171f777e5432384a2e2f", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001A8C1DD", "uniref100_id": "UniRef100_C4XG71", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C1DD", "UniRef:UniRef100_C4XG71" ] }, "ips": { "uniref100_id": "UniRef100_C4XG71", "uniref90_id": "UniRef90_C4XG71" }, "psc": { "uniref90_id": "UniRef90_C4XG71", "gene": "rpmA", "product": "50S ribosomal protein L27", "uniref50_id": "UniRef50_A0A3P8MF53", "kegg_orthology_id": "K02899", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8MF53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8MF53" ], "product": "Large ribosomal subunit protein bL27" }, "genes": [ "rpmA" ], "id": "OAIKOHHJFB_1748", "locus": "GCA000209735_01746" }, { "type": "cds", "sequence": "contig_1", "start": 979037, "stop": 979216, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGTTTCAAGTTATAAGTTTTCTCAATAAGTCAACTGACTCTTCTACGCTTAAAGAAGAATTAGTTATTTTTGAATATACAAGTCCATATAAACAACTTATTGTAATACCTGCTATTGTAGCTGCTGCTAAAATGATTAAGATTGTTCCAAAGACTCAATTCTTTCTTGCTATATGA", "aa": "MLFQVISFLNKSTDSSTLKEELVIFEYTSPYKQLIVIPAIVAAAKMIKIVPKTQFFLAI", "aa_hexdigest": "79b1208bcc328e84e83f8063087d7015", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6678.974700000001, "isoelectric_point": 9.158330345153807 }, "id": "OAIKOHHJFB_1749", "locus": "GCA000209735_01747" }, { "type": "cds", "sequence": "contig_1", "start": 979195, "stop": 979401, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGCGCAGTTGCTATTTCAATGAAGGATGGATTTGCTGCTTGTAAAGAATTTATAAATAACATTTTTCATAATTCAACTTCAATTTCATTAATTGGTGGCTCAATTATTGCTTTAGTTGCAATTGCTTTATTCTTTATTCTTTCAACAGCTACAGTCATTGTTTACTTTGTAACATCAATAGCTCATATAGCAAGAAAGAATTGA", "aa": "MCAVAISMKDGFAACKEFINNIFHNSTSISLIGGSIIALVAIALFFILSTATVIVYFVTSIAHIARKN", "aa_hexdigest": "1464c39fe1539d6b33bed806147b9515", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7250.568299999998, "isoelectric_point": 8.80420551300049 }, "id": "OAIKOHHJFB_1750", "locus": "GCA000209735_01748" }, { "type": "cds", "sequence": "contig_1", "start": 979405, "stop": 979563, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATAGTAGAACTAAATTTATTCCGCTAATAGTATTATTAGCTTTTGTAGTTGGCTTTTGTTTATTGTTCATTTTTGGTATGCAACCTGAAATGATCATCAAAGAAGAAGCCAAACCAGCAACAACTGAATTTGGAGTAGGATTCTTTGGCACTTGA", "aa": "MYSRTKFIPLIVLLAFVVGFCLLFIFGMQPEMIIKEEAKPATTEFGVGFFGT", "aa_hexdigest": "10b16adc0eb81f538f845a036654f133", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5850.026299999999, "isoelectric_point": 6.047066307067872 }, "id": "OAIKOHHJFB_1751", "locus": "GCA000209735_01749" }, { "type": "cds", "sequence": "contig_1", "start": 979683, "stop": 979889, "strand": "-", "frame": 3, "gene": null, "product": "Alcohol dehydrogenase 1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P3G6" ], "nt": "TTGTTCGAAAAAGAATTAGCTGGATCAATCGTTGGTACAAGACTTGATTTACAAGAATCACTTCAATATGCTGCAGAAGGAAAAGTTAAATCAGAAGTTACAAAAGTTATTAAACTAGAAGAAGTTTCAGAAGTATTTGAAAAACTTCAAAAAGGTGAATTCTTAGGACGTGCTGTTATTGACTTTGGTTACAGAGGAAAGAAATAA", "aa": "MFEKELAGSIVGTRLDLQESLQYAAEGKVKSEVTKVIKLEEVSEVFEKLQKGEFLGRAVIDFGYRGKK", "aa_hexdigest": "cd2ec2b1850c9fbed62381028544c82f", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P3G6", "query_cov": 0.8823529411764706, "subject_cov": 0.9523809523809523, "identity": 0.8, "score": 95.5, "evalue": 1.12e-24, "valid": false, "product": "Alcohol dehydrogenase 1", "uniref50_id": "UniRef50_A0A3B0P3G6" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P3G6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P3G6" ], "product": "Alcohol dehydrogenase 1" }, "genes": [], "id": "OAIKOHHJFB_1752", "locus": "GCA000209735_01750" }, { "type": "cds", "sequence": "contig_1", "start": 979896, "stop": 980237, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00107.32" ], "nt": "ATGGTTGTTATCGGTGTTGGTGGACTTGGCCAATTAGCAATTCAATATGCTAAAGCAATGGGATATAGACCTATTGGTATCGACTTAACAGATGAAAAATGCGAATTAGCTTTAAAATCAGGAGCAGAATTTGCATTCAACTCTAAGAAAGATCCAAACTTTATTCAAAAACTTATTGAAAAGACTGGTGGTGGAGCTCACGCTGTAATTAACACATCAGTTGCTACAATTGCTGGTGAACAAGGCATGGAAATGCTTCGTCGTGGAGGACGTCAAGTATTAGTAGGCCTTCCATCAAAAGACAAACTTGGTAAAGACAACATTGGCGTTTCAGTATTCTGA", "aa": "MVVIGVGGLGQLAIQYAKAMGYRPIGIDLTDEKCELALKSGAEFAFNSKKDPNFIQKLIEKTGGGAHAVINTSVATIAGEQGMEMLRRGGRQVLVGLPSKDKLGKDNIGVSVF", "aa_hexdigest": "711b2e3fb273a542520c76c9ad915162", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00107.32", "name": "ADH_zinc_N", "length": 95, "aa_cov": 0.831858407079646, "hmm_cov": 0.9894736842105263, "evalue": 9.118791488053085e-26, "score": 86.43392181396484, "start": 8, "stop": 101 } ], "seq_stats": { "molecular_weight": 11905.738499999992, "isoelectric_point": 9.130608940124514 }, "id": "OAIKOHHJFB_1753", "locus": "GCA000209735_01751" }, { "type": "cds", "sequence": "contig_1", "start": 980238, "stop": 980408, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGCTTACACATTTGATGGATCATTTGCTGAATATGCAATTGGACACGAAGATTATGTAGGAATTGTTCCAGAAGGATTAGATTTAATTACTGGAGCACCTGTAGTATGTGCTGGTGTTACAACCTATAAGGCAATTAAAAGAGCTAAAGCTCGTCCAGGTTACTGA", "aa": "MSAYTFDGSFAEYAIGHEDYVGIVPEGLDLITGAPVVCAGVTTYKAIKRAKARPGY", "aa_hexdigest": "7bc716c17e1ab59e296874f5d62d1db6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5941.698300000004, "isoelectric_point": 5.563707542419433 }, "id": "OAIKOHHJFB_1754", "locus": "GCA000209735_01752" }, { "type": "cds", "sequence": "contig_1", "start": 980700, "stop": 980804, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTATAAAACATTAAAAAATTACTATTATTTTTTTAAGGAGATAGATTTTATGGCAAAAACAACTATGAAAGCTTTTGTTGTTGAAAAACCAAAACAATGA", "aa": "MFYKTLKNYYYFFKEIDFMAKTTMKAFVVEKPKQ", "aa_hexdigest": "d2de4e8ac69d91b8acd5697a4390473f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4280.078300000001, "isoelectric_point": 9.574538230895993 }, "id": "OAIKOHHJFB_1755", "locus": "GCA000209735_01753" }, { "type": "cds", "sequence": "contig_1", "start": 981078, "stop": 981368, "strand": "+", "frame": 3, "gene": null, "product": "Transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET5" ], "nt": "ATGGAAAACTCTTATTCAGCATGCTATTCAGTAATAGAAAAATACAAAAAGTTTGGTTATAATTCTTTTGCTATGGAAAAGAAAAAAGGAAGAAAATCTAAAATAAATTTAGATGCTCAAAAGGCAACAAATTTTAAAATCAATATTGAAAATAAAATAGAAAATAAAGATTTATTAATTAAACAATTAAAGGAAGAAAATAAAATACTCAAATTGGAGAATGCGATAGCAAAAAAAGTGAGCGCCTTGGTTCAATTGAAAGACTCACTAACAAAGAAAAATTCCAAATAA", "aa": "MENSYSACYSVIEKYKKFGYNSFAMEKKKGRKSKINLDAQKATNFKINIENKIENKDLLIKQLKEENKILKLENAIAKKVSALVQLKDSLTKKNSK", "aa_hexdigest": "ba35629af28b55b0957136a2608bd32f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XET5", "query_cov": 1.0208333333333333, "subject_cov": 0.9607843137254902, "identity": 0.765, "score": 129.0, "evalue": 4.04e-37, "valid": false, "product": "Transposase", "uniref50_id": "UniRef50_C4XET5" }, "pscc": { "uniref50_id": "UniRef50_C4XET5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET5" ], "product": "Transposase" }, "genes": [], "id": "OAIKOHHJFB_1756", "locus": "GCA000209735_01754" }, { "type": "cds", "sequence": "contig_1", "start": 981631, "stop": 981921, "strand": "+", "frame": 1, "gene": null, "product": "Transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET5" ], "nt": "ATGGAAAACTCTTATTCAGCATGCTATTCAGTAATAGAAAAATACAAAAAGTTTGGTTATAATTCTTTTGCTATGGAAAAGAAAAAAGGAAGAAAATCTAAAATAAATTTAGATGCTCAAAAGGCAACAAATTTTAAAATCAATATTGAAAATAAAATAGAAAATAAAGATTTATTAATTAAACAATTAAAGGAAGAAAATAAAATACTCAAATTGGAGAATGCGATAGCAAAAAAAGTGAGCGCCTTGGTTCAATTGAAAGACTCACTAACAAAGAAAAATCCAAAATAA", "aa": "MENSYSACYSVIEKYKKFGYNSFAMEKKKGRKSKINLDAQKATNFKINIENKIENKDLLIKQLKEENKILKLENAIAKKVSALVQLKDSLTKKNPK", "aa_hexdigest": "aa99a4ec9aadfdef32c97828b34d22de", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_C4XET5", "query_cov": 1.0208333333333333, "subject_cov": 0.9607843137254902, "identity": 0.755, "score": 127.0, "evalue": 2.34e-36, "valid": false, "product": "Transposase", "uniref50_id": "UniRef50_C4XET5" }, "pscc": { "uniref50_id": "UniRef50_C4XET5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XET5" ], "product": "Transposase" }, "genes": [], "id": "OAIKOHHJFB_1757", "locus": "GCA000209735_01755" }, { "type": "gap", "sequence": "contig_1", "start": 982021, "stop": 982120, "strand": ".", "length": 100, "id": "OAIKOHHJFB_1812" }, { "type": "cds", "sequence": "contig_1", "start": 982297, "stop": 982575, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH8", "UniRef:UniRef90_C4XEH8" ], "nt": "TTGTTAGATATATTAAATACAATTTATCTCTATTTTAGAGACTCTTTTGTTTTGAGCGATATTGATAGATACAATTTAAATAGTTTGATAAGATACACATCTAAACTTGAAAGTGGAATCATTAATAATGATTATAGTTCACTAATAGAAGGACACATATCTGTTTATATTAAAAGAAAACTAAACAAAAGCTTTGCATTATATTCTCAAAAAACAATAGAAGCTTTATTGTTGAATGATAATAAAATTCAAAATACTCTTATTCAGACATATCATTAG", "aa": "MLDILNTIYLYFRDSFVLSDIDRYNLNSLIRYTSKLESGIINNDYSSLIEGHISVYIKRKLNKSFALYSQKTIEALLLNDNKIQNTLIQTYH", "aa_hexdigest": "5dd97fea0cd43f7cf7d9092425b8453f", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_C4XEH8", "query_cov": 1.0, "subject_cov": 0.8141592920353983, "identity": 0.9890000000000001, "score": 177.0, "evalue": 2.98e-56, "valid": true, "uniref50_id": "UniRef50_C4XEH8" }, "pscc": { "uniref50_id": "UniRef50_C4XEH8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XEH8" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10826.261399999988, "isoelectric_point": 7.949612617492677 }, "id": "OAIKOHHJFB_1758", "locus": "GCA000209735_01756" }, { "type": "cds", "sequence": "contig_1", "start": 982877, "stop": 983131, "strand": "+", "frame": 2, "gene": null, "product": "Helix-turn-helix domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFJ2", "UniRef:UniRef90_C4XG76" ], "nt": "ATGGAAAACTCTTATTCAGCATGCTATTCAGTAATAGAAAAATACAAAAAGTTTGGTTATAATTCTTTTGCTATGGAAAAGAAAAAAGGAAGAAAATCTAAAATAAATTTAGATGCTCAAAAGGCAACAAATTTTAAAATCAATATTGAAAATAAAATAGAAAATAAAGATTTATTGGAAGTGATAGAAAGAAGTGTGTTGAACACTTTTTTTATTTCACTTTTGATTATTTATAAAAGTACTCATAAACATTAA", "aa": "MENSYSACYSVIEKYKKFGYNSFAMEKKKGRKSKINLDAQKATNFKINIENKIENKDLLEVIERSVLNTFFISLLIIYKSTHKH", "aa_hexdigest": "69c351ec48c5e3020f31cfdaa84e8132", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 982667, "stop": 983131, "observations": { "mutation": [ 982694, 982772 ], "stop": [ 982694, 982772 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG76", "query_cov": 1.0, "subject_cov": 0.5454545454545454, "identity": 1.0, "score": 162.0, "evalue": 9.74e-50, "gene_start": 1, "gene_end": 84, "reference_start": 71, "reference_end": 154, "reference_sequence": "MVDRKRKLEWTPNSGLFGKGFIFMQFKFKKVKRNKWNRDIKGYLKLKLDQKIKIIELYFQEFSILEISKIMENSYSACYSVIEKYKKFGYNSFAMEKKKGRKSKINLDAQKATNFKINIENKIENKDLLEVIERSVLNTFFISLLIIYKSTHKH" }, "paralog": false, "identity": 0.9805194805194806, "score": 296.0, "evalue": 5.48e-105, "description": "Internal stop codon at 982694, 982772. Nonsense mutation around 982694, 982772.", "psc": { "uniref90_id": "UniRef90_C4XG76", "product": "Helix-turn-helix domain-containing protein", "uniref50_id": "UniRef50_C4XFJ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG76", "UniRef:UniRef50_C4XFJ2" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFJ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFJ2" ], "product": "Transposase" } }, "id": "OAIKOHHJFB_1759", "locus": "GCA000209735_01757" }, { "type": "gap", "sequence": "contig_1", "start": 983582, "stop": 983681, "strand": ".", "length": 100, "id": "OAIKOHHJFB_1813" }, { "type": "gap", "sequence": "contig_1", "start": 983726, "stop": 983726, "strand": ".", "length": 1, "id": "OAIKOHHJFB_1814" }, { "type": "cds", "sequence": "contig_1", "start": 984304, "stop": 984534, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013354584.1", "SO:0001217", "UniParc:UPI0001E330CC", "UniRef:UniRef100_A0AB32XBB6", "UniRef:UniRef50_A0AB32XBB6", "UniRef:UniRef90_A0AB32XBB6" ], "nt": "TTGAAAAGCAATGCTAAAGTTAAATCAAAAATTAAACAAGTAAAAATTAATAATTTCAAAATAGTTAAATTAACAAAATCTTTTGAAGAATTATTTTTTGGAGTTAATGATTCTTCAAAATTATCGGCCAATAACGTTATAACTTTAGATCAATATGTTGATAATTTAAACTTATTTCAGAGCAAAATTAATCTTAATTTAAATTTAATTAATAAAGAACTAAAAATTTAA", "aa": "MKSNAKVKSKIKQVKINNFKIVKLTKSFEELFFGVNDSSKLSANNVITLDQYVDNLNLFQSKINLNLNLINKELKI", "aa_hexdigest": "a839b59558589508e25b624123782c0d", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001E330CC", "ncbi_nrp_id": "WP_013354584.1", "uniref100_id": "UniRef100_A0AB32XBB6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001E330CC", "RefSeq:WP_013354584.1", "UniRef:UniRef100_A0AB32XBB6" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XBB6", "uniref90_id": "UniRef90_A0AB32XBB6" }, "psc": { "uniref90_id": "UniRef90_A0AB32XBB6", "uniref50_id": "UniRef50_A0AB32XBB6" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XBB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XBB6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8730.137599999995, "isoelectric_point": 9.875218772888182 }, "id": "OAIKOHHJFB_1760", "locus": "GCA000209735_01758" }, { "type": "cds", "sequence": "contig_1", "start": 984998, "stop": 985330, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_013354585.1", "SO:0001217", "UniParc:UPI0001A8C1FC", "UniRef:UniRef100_C4XG79", "UniRef:UniRef50_C4XG79", "UniRef:UniRef90_C4XG79" ], "nt": "ATGTTTTCAATAGTTGCTAATGATAAATTTTTGGGCCTAGCAGAAAGTGCCAAAAAATTTAAATATGTATTACAAGATTTAAAAAATAAAGGCGCTACTAACTACGAATGTGTTGAACGAAATATTTTAAAAACCGTATTTAATAGTAAAAAAACACAAATATTTTTATATCAAGAAAATAATAATTTGAGAGCAGAAAATATTGTTAATTTCAATCTTAAATATTTTAGCAATAGTGTTAAAAATGAATTAAATGATTCTTTACTTTCTAATGATGATTTAATAAAATTAAGCTTGAAAAAGCTTATGTTGAAATTACCAAAATATCTTTAA", "aa": "MFSIVANDKFLGLAESAKKFKYVLQDLKNKGATNYECVERNILKTVFNSKKTQIFLYQENNNLRAENIVNFNLKYFSNSVKNELNDSLLSNDDLIKLSLKKLMLKLPKYL", "aa_hexdigest": "8efe922e4c33b3d09deb2d5d138b68db", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001A8C1FC", "ncbi_nrp_id": "WP_013354585.1", "uniref100_id": "UniRef100_C4XG79", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001A8C1FC", "RefSeq:WP_013354585.1", "UniRef:UniRef100_C4XG79" ] }, "ips": { "uniref100_id": "UniRef100_C4XG79", "uniref90_id": "UniRef90_C4XG79" }, "psc": { "uniref90_id": "UniRef90_C4XG79", "uniref50_id": "UniRef50_C4XG79" }, "pscc": { "uniref50_id": "UniRef50_C4XG79", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG79" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12802.756799999983, "isoelectric_point": 9.520449256896974 }, "id": "OAIKOHHJFB_1761", "locus": "GCA000209735_01759" }, { "type": "cds", "sequence": "contig_1", "start": 985715, "stop": 987700, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG80", "UniRef:UniRef90_C4XG80" ], "nt": "ATGTCTGATGATGATTTTCTAAAAAATTTAGAAATTAAAGGACAAAATGTAAGTGAAATTTATGCTAGTGATGCAAAAATTACAAATGTTGAATACAAAAAAGATTCTAATTATGAAATTAATGTTTCTCTAGAAGGATCAAATGTTCTAAAATCAACTGCATACTACAAAATTGAAATTACAAATAAAAATACAAAAGTTAAAAAGTCAAAAATTGTTCCAATAAGTGGTTTCAAGAAGTTTGATGAAAAAAGTACTATTTTAAAAGAAAAAGAGCTATTTGAAGATAAATCTAAATTAAAAGAATATTTAGATAATATGCCTAAAAATGATTCTGAAAATCTTAAAAAATATATTTCTAAAAATAAACATTTTATTTACAACGAAATACCAAGTTCATTAAAAGCTTCATTTAAAGGTTATTCTTTCATTCAATCTAATGGTCAAGATGCTGTTAGAGTTGTTTTCGTTTTAAATGGTCATATTACAAATGATAATCTCGAAAATAATATAGTTAAAAAAGCAAATAAAGAATATAAAAATAATGAAAAAACTTTTGAATTTTACTTAAGTAATTTAGTAAAACAAGGAACATCAAGTTCAGAAATTCAAGCAAGAAATAACTTAAGAGATTTAAAAGAAAAATACGAAACTCTTAAAAAAGAAGCTGAAGTAGCTAAATCGAAAGTTACAGGTGCTATTGTTGCTGAATATGAAAATGTTTTAGTTTCAATTACTAAAAATATTAATGAAATCGACAAAATTTTAAATGATGCAACAATTAAAGCAGCAGATCATGTTGATACATTAAAAGTTAATTATGAAAAAGACTTATTAGATAAAAATACTGTAGATTTAAAAGCAACAATTAATGATGCTGAAGTTACAAGAGATAACTATAAAGCTAAAGATGAAACAAATTATAACAATCCAATAATTGAATTTAACAAAGCTATTGATCAAGCAAAAATTAAACTTGCTTCAACAAATATTAAAGAAATTAATGATGAAATTTCTAACTTAAAAACTAAAAAAGAAGAGTTTATTAATGCTTTAAAAACTATCTTAACTGACACACAAAGTGATTCAAAAACTAAATTGTTAGAATCAAGCGATTACAAATATTTGATCGATGATATTAAAGCTCGTAAAGCTACTTTTGATGCTACACTTAATGAAGATAATATTCTAAGTGAAATTGAAAACTTAGAAGTTTTAGTTCAAAATACTCAAACAATTGATGCTATTACACCAAAAGCAATAATTGAAGCTAATAATAAACTTCTAGCTTCCTTACTAGCCTATAAAACAGCCTTAGAAGCAAAATTACACAAAATAAAACAAGATGTAATAGACTCAACTTATGACAAAGATTCATTGTCACAATTTATCGAATATTTAATTAAAAAAATTAAAACAACAAATGAAAGCAAAGCTTTAGTTAATAAACAAATAGATTTATTTAAAGCTTATTTAACTACACCAGGTTTTGTTCAAAAATATAATTCTTTAGAAAAAGATTTTGATAAATATGAAGCAATTTTTAATGAAAATATAAATAAAGCTAATGAACTTATCGCAACTCTAGATGCAGGATTAGATCAATATTCAAATTCAAAAGATAAATATAGTGAAATCAAAACTAAAGTTGACTCACTTTTAGCAGAACAAGCAACTCGTTTAAGCTCAATTGGAACTCAAATTGTTAATAGTGCACTTATTGATTTTGATAATTATGTTCAAAAGCAAAAAGCTTTAATCAATACTTCACGTAATAACTACAAAACAATTGTGACAAATGTTTACAATTTATTCAAAGATTCAGAAATCTACAAACAATATATAGATGATAATAAAGCGAAACTTACAAACTCAACTATTGATGTTAAAACTGCTTTTGAATTATTAAAAACTTTATATACAAAAGATAATGAAATGAATCAAAAAGTTGCAGCTCACTCAAACTTAGCAACTAATAGCTACTGA", "aa": "MSDDDFLKNLEIKGQNVSEIYASDAKITNVEYKKDSNYEINVSLEGSNVLKSTAYYKIEITNKNTKVKKSKIVPISGFKKFDEKSTILKEKELFEDKSKLKEYLDNMPKNDSENLKKYISKNKHFIYNEIPSSLKASFKGYSFIQSNGQDAVRVVFVLNGHITNDNLENNIVKKANKEYKNNEKTFEFYLSNLVKQGTSSSEIQARNNLRDLKEKYETLKKEAEVAKSKVTGAIVAEYENVLVSITKNINEIDKILNDATIKAADHVDTLKVNYEKDLLDKNTVDLKATINDAEVTRDNYKAKDETNYNNPIIEFNKAIDQAKIKLASTNIKEINDEISNLKTKKEEFINALKTILTDTQSDSKTKLLESSDYKYLIDDIKARKATFDATLNEDNILSEIENLEVLVQNTQTIDAITPKAIIEANNKLLASLLAYKTALEAKLHKIKQDVIDSTYDKDSLSQFIEYLIKKIKTTNESKALVNKQIDLFKAYLTTPGFVQKYNSLEKDFDKYEAIFNENINKANELIATLDAGLDQYSNSKDKYSEIKTKVDSLLAEQATRLSSIGTQIVNSALIDFDNYVQKQKALINTSRNNYKTIVTNVYNLFKDSEIYKQYIDDNKAKLTNSTIDVKTAFELLKTLYTKDNEMNQKVAAHSNLATNSY", "aa_hexdigest": "165cb101c63a783fac8c2633e6600e0c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 985595, "stop": 988003, "observations": { "mutation": [ 985637, 987698 ], "stop": [ 985637, 987698 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG80", "query_cov": 1.0, "subject_cov": 0.7099892588614393, "identity": 1.0, "score": 1223.0, "evalue": 0.0, "gene_start": 1, "gene_end": 661, "reference_start": 41, "reference_end": 701, "reference_sequence": "MSTKTISKATKGLGWAIATATITGSILLTIIAIKTQNTKKMSDDDFLKNLEIKGQNVSEIYASDAKITNVEYKKDSNYEINVSLEGSNVLKSTAYYKIEITNKNTKVKKSKIVPISGFKKFDEKSTILKEKELFEDKSKLKEYLDNMPKNDSENLKKYISKNKHFIYNEIPSSLKASFKGYSFIQSNGQDAVRVVFVLNGHITNDNLENNIVKKANKEYKNNEKTFEFYLSNLVKQGTSSSEIQARNNLRDLKEKYETLKKEAEVAKSKVTGAIVAEYENVLVSITKNINEIDKILNDATIKAADHVDTLKVNYEKDLLDKNTVDLKATINDAEVTRDNYKAKDETNYNNPIIEFNKAIDQAKIKLASTNIKEINDEISNLKTKKEEFINALKTILTDTQSDSKTKLLESSDYKYLIDDIKARKATFDATLNEDNILSEIENLEVLVQNTQTIDAITPKAIIEANNKLLASLLAYKTALEAKLHKIKQDVIDSTYDKDSLSQFIEYLIKKIKTTNESKALVNKQIDLFKAYLTTPGFVQKYNSLEKDFDKYEAIFNENINKANELIATLDAGLDQYSNSKDKYSEIKTKVDSLLAEQATRLSSIGTQIVNSALIDFDNYVQKQKALINTSRNNYKTIVTNVYNLFKDSEIYKQYIDDNKAKLTNSTIDVKTAFELLKTLYTKDNEMNQKVAAHSNLATNSYWNSLLDGDLKNNFTIPMQVNADYENYFKLDDHYKMALDIIMDNLISQIRSEHIKGIEAKQEEIYRIFYEKMRAIYNAQLTNGVKFVNDNILDAKNRRIQALKEYEKALKEAENLIKHYTDEHYNNTDDFFKTLEEGIKAVKTLNKDRAMLSLVKNTTIELKEVIKNSQDQYRTFLLSKLDEARKNWQIYSNSINGPEHQDVFPKTIKLINESWKKLKMLLTIVQLLLLKN" }, "paralog": false, "identity": 0.9975062344139651, "score": 1540.0, "evalue": 0.0, "description": "Internal stop codon at 985637, 987698. Nonsense mutation around 985637, 987698.", "psc": { "uniref90_id": "UniRef90_C4XG80", "uniref50_id": "UniRef50_C4XG80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG80", "UniRef:UniRef50_C4XG80" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG80" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 75369.16350000027, "isoelectric_point": 6.638475990295411 }, "id": "OAIKOHHJFB_1762", "locus": "GCA000209735_01760" }, { "type": "cds", "sequence": "contig_1", "start": 987746, "stop": 988255, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGTTAATGCTGATTATGAAAATTACTTTAAATTAGATGACCATTACAAAATGGCTTTAGATATTATTATGGACAATTTAATATCTCAAATTCGTTCAGAACATATTAAAGGTATTGAAGCGAAACAAGAAGAAATTTATCGTATTTTCTATGAAAAAATGCGTGCTATTTATAATGCACAATTAACTAATGGAGTTAAATTTGTAAATGATAATATTCTAGATGCTAAAAATAGAAGAATTCAAGCATTAAAAGAATATGAAAAGGCTTTAAAAGAAGCAGAAAATTTAATCAAACATTATACAGATGAACACTATAACAATACTGATGATTTCTTCAAAACACTTGAAGAAGGCATTAAAGCTGTTAAAACTTTAAATAAAGATCGAGCAATGCTAAGTCTTGTAAAAAATACAACTATAGAATTAAAAGAAGTTATCAAAAATTCACAAGATCAATATAGAACATTCCTATTGTCTAAACTTGATGAAGCTAGAAAAAATTGA", "aa": "MQVNADYENYFKLDDHYKMALDIIMDNLISQIRSEHIKGIEAKQEEIYRIFYEKMRAIYNAQLTNGVKFVNDNILDAKNRRIQALKEYEKALKEAENLIKHYTDEHYNNTDDFFKTLEEGIKAVKTLNKDRAMLSLVKNTTIELKEVIKNSQDQYRTFLLSKLDEARKN", "aa_hexdigest": "42864cbd500023e603dc397f908f3820", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20091.6405, "isoelectric_point": 6.448179054260252 }, "id": "OAIKOHHJFB_1763", "locus": "GCA000209735_01761" }, { "type": "cds", "sequence": "contig_1", "start": 988951, "stop": 990090, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAAAAACAATAGTTTAGCAGCTGCTAAAAATAAATTACAATCATCAATTGAAAAGGTTGAAAATCATTTAAATTCAAGAAATTCTTTAGACAATTTAGATAATTCATATTACTCAAATTTAATGCAAAATGAGAAAATTGATTACAATGAAAATAGTTCAACTGCTGCTTCCAAAAAAGATTTAAAATCTATATTAGATTCAATGAAAAATATTGTTAAAGGCAATGATGAAAATGCAATTAATCACAATGTTCAATTTGGTCAAAAATTAATTGATGCTATTGAAGAAAAAAGAACAGAAGACATAGTTGCAAAACAAAATACATTAAAAGATAAATTAAATGAATATAACGATTTAAAAGATTCATTAAAATCATTATCTTCAAAAGATTATTATGGAGCTCAAATTACAAATCTTGAAGCTAATTCAACTGCTGATGAAGATGCTGATAGAATTACAAAATTAGCACGAGCTAACTTAATTAAAGAAAGCCAAAAATCAATTGTTAATACTAAAGTTGAATTAGAAAAATTAATTGGTAATTTAACTAAAATTAAAACTGATCGTGAAGCTCTCATTCGACAAGCAAATGATCAATATAATACAAATAAAGACAAATTTGCTGAGCTTCAAAAACAACTAAATGATTTAAATATTACACCTGCTACAATAGCGGATTATAACACAAAAGTGAAACCTAAACTTGAAGCAGCAAATAATAAAAATGAATTATTAAAAGAAGTTTCTAAAGATCAAAATAATAGTAATATAACAGCTAAAATTAATGATTCTAAAGTTGCAATTCAAGATTTAGAAAATGTTGTAAACAACTATAAAACAGCTCTTAACAACAAAATTAAAGAAAATATTTCAGCAATAAAAGAATTAGTACAATACATTAGAAACAATCCAAACAATTTAACTCTTCCAGCTAATTTTGTAACCAATTTAAAAGCTGTAGAAGACAATCAAGACTCAACTGATATTGCTATTTTAAATCAAACAAAAACTCAAAGTGAAGCATTAATTAAAGAGTTAACCGATTTAATTAATACAGCAGTAGCAACTGTTAATAATAAAGCTCAAGATACAAATCAAAAAGTTGAAGAAGAAAAAGCAAAAAATGAAACATGA", "aa": "MNKNNSLAAAKNKLQSSIEKVENHLNSRNSLDNLDNSYYSNLMQNEKIDYNENSSTAASKKDLKSILDSMKNIVKGNDENAINHNVQFGQKLIDAIEEKRTEDIVAKQNTLKDKLNEYNDLKDSLKSLSSKDYYGAQITNLEANSTADEDADRITKLARANLIKESQKSIVNTKVELEKLIGNLTKIKTDREALIRQANDQYNTNKDKFAELQKQLNDLNITPATIADYNTKVKPKLEAANNKNELLKEVSKDQNNSNITAKINDSKVAIQDLENVVNNYKTALNNKIKENISAIKELVQYIRNNPNNLTLPANFVTNLKAVEDNQDSTDIAILNQTKTQSEALIKELTDLINTAVATVNNKAQDTNQKVEEEKAKNET", "aa_hexdigest": "20df83ae047d637d41546dec7d96aec7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 42602.92980000001, "isoelectric_point": 5.776114845275878 }, "id": "OAIKOHHJFB_1764", "locus": "GCA000209735_01762" }, { "type": "cds", "sequence": "contig_1", "start": 990091, "stop": 990453, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGCTGCTCAAGTTCGAGAAATTCAACAACTTTTTGATAATTCTCAAGCCTTATTTAATGAAGTTAAAAACGCTGCTAATAATAAAGATCAAATCAAAGATGTAGGTGTTGATAACTTAACAAATAATATAACTAAAGCAGAAGCAAAATTAAAAGAAATTCAAAACAAAAAAGCAGAATTTAAACAAGAAAATACTAATTTAAGAAATCAAATTAGTGAATTAATGAATGATTTAAAAGTACAAGCTGATACACTTCCAAATGAATTTAATGAACACATTTTAGCAACTCATAGCTCAATAAAAACCATTCAAGAATATTATGAAAATACATATAGCAAAATTAAAAATCAAGAATGA", "aa": "MTAAQVREIQQLFDNSQALFNEVKNAANNKDQIKDVGVDNLTNNITKAEAKLKEIQNKKAEFKQENTNLRNQISELMNDLKVQADTLPNEFNEHILATHSSIKTIQEYYENTYSKIKNQE", "aa_hexdigest": "ab596ef062621d7a619a955054727c3e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13842.224799999987, "isoelectric_point": 5.450825309753417 }, "id": "OAIKOHHJFB_1765", "locus": "GCA000209735_01763" }, { "type": "cds", "sequence": "contig_1", "start": 990604, "stop": 991875, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00217F41A6", "UniRef:UniRef90_UPI00217F41A6" ], "nt": "TTGACTACCGCTAATGATGAATTAACTGGTATTAATAAAGTTGATAACTATTCAGATTCGATTACTAAATTAAATAATCAAAAGAAAGCAGAATATCAAGAATTAATTGATCAAAATAACAATGATCTTAAAACTCTTAAAAATGCTTTAATTGAAATTAAAAATAGTAATGACTTAATTGAAATATTAATTAATGAAGTTGTCAATAAAAAATGTAACTTAATTAATGCAACTATTAAAGAATATGAAGATCAACTAGAAGCAATTAATAATTCTATTTACAGCGATTTATATGAAGACATTACAACAAGTTTACATTTAGACTTTTTAAACTCTTTAGCTATTTTAAAAAATAAATATCCAAATAATTTAAATAAAGTAATCAAACTTTCAGAAGTAAAAAGAGATTATGAAGAAATTGAAGGTAAACTTAATGATTTAAAAAATAGATTAGCCGCTACACAAAATGAAAGAAATGACATTCTTGCAGAATTAAGATTAAACTTTGACAAAATTAATTCATATGCTAATTATTCAGATGCTGATAGTAAATTGCCTTTAACTAAAGCTTTAATAAATGATGCAAATAATAATTTAGAAAATCACTTTAGTTCAATGACACTAAAATACTTAAAAGCTGAAAAATCTGCTAGTGGTCAATATACAAAAGTAATAAATGAAATAAATCAATATGAAGCAGATCTTAATGTAGCTTTACAGGAAATTATTAATGAAGTAAAAGATGCTAAATATCAAAAAATAGATGACATTATTTCAAATAATTCAAGTGTCTTTTTAGCTGACAAAGATGATTATAAAAAAACAATTGCACCAGTTAAACAAAAAGCTAATAATGGTGTTAATGACTTAAAAACTTTAATTACAACAATTAAAGATGCAAATGAAAAACTTGTAGCTATTAAAGCTCTTGGCAATAGAATTCTTACAAAAATTAATACTGAATTCAACAATAGAATTAACCGACTTAAAACTATTGCAAATGAAACTGATGGATTCTTAAGTCCTAAAGTTGCTGACGGTAAATATGAAGGTTCTATTTTGCATGGAATTAAACAATCAAATGATGAAATTATTAAAATAATTAAAACAAAAATCGATACAATAATGAACAATAATAGTAACCTTGCATACTTAGTAAAAATCGATAATTCTAGTGATAAAACTATAGAAAAATTAGATCAATTAATTGCAAGAGCAACCCAATTTAAAGATAACGCAATCACAAAAACTAATGAATATGAAAGAGCATGA", "aa": "MTTANDELTGINKVDNYSDSITKLNNQKKAEYQELIDQNNNDLKTLKNALIEIKNSNDLIEILINEVVNKKCNLINATIKEYEDQLEAINNSIYSDLYEDITTSLHLDFLNSLAILKNKYPNNLNKVIKLSEVKRDYEEIEGKLNDLKNRLAATQNERNDILAELRLNFDKINSYANYSDADSKLPLTKALINDANNNLENHFSSMTLKYLKAEKSASGQYTKVINEINQYEADLNVALQEIINEVKDAKYQKIDDIISNNSSVFLADKDDYKKTIAPVKQKANNGVNDLKTLITTIKDANEKLVAIKALGNRILTKINTEFNNRINRLKTIANETDGFLSPKVADGKYEGSILHGIKQSNDEIIKIIKTKIDTIMNNNSNLAYLVKIDNSSDKTIEKLDQLIARATQFKDNAITKTNEYERA", "aa_hexdigest": "f639a5a7d28221397cf1996faf0ae93d", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 990304, "stop": 992178, "observations": { "mutation": [ 990451, 991873, 991879 ], "stop": [ 990451, 991873, 991879 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00217F41A6", "query_cov": 1.0, "subject_cov": 0.4890173410404624, "identity": 0.95, "score": 751.0, "evalue": 1.02e-265, "gene_start": 1, "gene_end": 423, "reference_start": 173, "reference_end": 595, "reference_sequence": "MNDSCSSSRNSTTFDNSQALFNEVKNAANNKDQIKDVGVDNLTNNITKAEAKLKEIQNKKAEFKQENTNLRNQISELMNDLKVQADTLPNEFNEHILATHSSIKTIQEYYENTYSKIKNQEWENKNINDLQAIKDILDGYKQSLDNYKNEAAAAYRNELKEQIQKSNNKVQKLTTANDELTGINKVKNYSDLITKLNNQKKAEYQELIDQNNNVLKTLKDALIEIKNSNNLVETLINEVVNEKCNSINAIIKKYEDQLEAINNSIYSDLYEDITTSLHLDFLNSLATLKNKYPDNLNKVIKLSEVKRDYEEIKSKLDDLNNRLAATQNERNNILAELRLNFDKINSYANYLDADTKLPLTKALINDANNNLENHFSSMTLKYLKAEKSASGQYTKVINEINQYEADLNVALQEIINEVKDAKYQKIDDIISNNSSVFLADKDDYKKTIAPVKQKANNGVNDLKTLITTIKDANEKLVAIKALGNRILTKINTEFNNRINRLKTIANETDGFLSPKVADGKYEGSILHGIKQSNDEIIKIIKTKIDTIMNNNSNLAYLVKIDNSSDKTIEKLDQLIARATQFKDNAITKTNEYERAWEEAKNENLQKLDEANGIIQFLNDKKTTSTITSEEQTALTALNTAKQNYDRIIENNIDHKSKTKIEDATNHLNDALNTARQELKNSQKTTAIINLKNLKSEINADLAKLKGYSSLSNDLTKSIGDSLVQYKASLQKVIDEIDDLVQKINTIGFVQSEYDTVYNKYKDVPNKDENIIKFIENKVFVETKFLNKYDIELDEITNQELKDKVTAKFNEFKADIVNFCSQNITNYDADVATLTTKHQTIQNKWNVDSEMIKIGKYLKYVVVKQL" }, "paralog": false, "identity": 0.9567307692307693, "score": 1153.0, "evalue": 0.0, "description": "Internal stop codon at 990451, 991873, 991879. Nonsense mutation around 990451, 991873, 991879.", "psc": { "uniref90_id": "UniRef90_UPI00217F41A6", "uniref50_id": "UniRef50_UPI00217F41A6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00217F41A6", "UniRef:UniRef50_UPI00217F41A6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00217F41A6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00217F41A6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 47995.530699999996, "isoelectric_point": 5.4840192794799805 }, "id": "OAIKOHHJFB_1766", "locus": "GCA000209735_01764" }, { "type": "cds", "sequence": "contig_1", "start": 992119, "stop": 992619, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAATTCACAAAAAACTACTGCAATTATTAATTTAAAAAACCTTAAATCAGAAATTAATGCAGACTTAGCAAAACTAAAAGGCTATTCATCATTATCAAATGATCTTACTAAATCAATTGGTGATAGCTTAGTTCAATATAAAGCTTCATTACAAAAAGTAATTGATGAAATTGATGATTTAGTTCAAAAAATCAATACCATAGGTTTTGTTCAAAGTGAATATGATACTGTTTATAACAAATATAAAGATGTACCAAATAAAGACGAAAACATCATTAAATTTATTGAAAATAAAGTATTTGTTGAAACTAAATTCTTAAACAAATATGACATCAAATTAGATGAAATAACTAATCAAGAATTAAAAGATAAAGTTACAACTAAATTTAATGAATTCAAGGCAGATATAGTTAACTTCTGTTCACAAAATATTACTAATTATGATGCTGATGTTGCAACACTTACAACAAAACATCAAGCAATTCAAAATAAATGA", "aa": "MKNSQKTTAIINLKNLKSEINADLAKLKGYSSLSNDLTKSIGDSLVQYKASLQKVIDEIDDLVQKINTIGFVQSEYDTVYNKYKDVPNKDENIIKFIENKVFVETKFLNKYDIKLDEITNQELKDKVTTKFNEFKADIVNFCSQNITNYDADVATLTTKHQAIQNK", "aa_hexdigest": "06df3394339a258b2cd19331fb00a180", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19013.329100000003, "isoelectric_point": 6.598859214782716 }, "id": "OAIKOHHJFB_1767", "locus": "GCA000209735_01765" }, { "type": "cds", "sequence": "contig_1", "start": 992635, "stop": 992886, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAAAATTGGTAAATACCTTAAATATGTTGTTGAAGCAACAAACTTTGAAAAAGCTATTCAAGCAATTGAAAATTCATATAACAATGAACCTAGCGGATACAAAGATGTTGAAGCTTACAATAACTTCAAAAAAGAAGAACAAAACTTAGCTTCTGGAAAAACATCTATTGAAACAATACTTAACAATTTCAAAAACAAAAAACAAAGTGAATTAATTAGTGGAAATGTTCCTGAAGATGCTCAATGA", "aa": "MIKIGKYLKYVVEATNFEKAIQAIENSYNNEPSGYKDVEAYNNFKKEEQNLASGKTSIETILNNFKNKKQSELISGNVPEDAQ", "aa_hexdigest": "01ee9d39c6e4db39b23adda7938e5d25", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9421.390299999994, "isoelectric_point": 5.436388206481933 }, "id": "OAIKOHHJFB_1768", "locus": "GCA000209735_01766" }, { "type": "cds", "sequence": "contig_1", "start": 992920, "stop": 993279, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAATAATAATCAAATAATAACAAATAAATTGCGTGAATTTGATGAACAAAATATTGAAAAATTAAGAAACTATGTTGAAGAACTTAGACAATTAGTAAATATTTATGAACATGCTTCAATAAAATTTGCAAGCACAAATTTAAATGACATTAATAAAGAAATTAGTGAAGTTGATGCTTTAATTGCAAATAGCAAAAAAGCCTATAAAACAGAAGATAAAGTAAAAAATAGCGCTGCAAACTTAAGTCAAAAAACACTTAATACTTACAATGAATTTAGAACTCAAACACTGAATAAATATAAACAATTAATGAGTCTAAATGTTACTCCTGTTGTAGCAAATGTTAGACCTTGA", "aa": "MSNNNQIITNKLREFDEQNIEKLRNYVEELRQLVNIYEHASIKFASTNLNDINKEISEVDALIANSKKAYKTEDKVKNSAANLSQKTLNTYNEFRTQTLNKYKQLMSLNVTPVVANVRP", "aa_hexdigest": "fc80d43e29d81d59d8ae6aedc9c0c765", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13779.387299999977, "isoelectric_point": 9.125387001037598 }, "id": "OAIKOHHJFB_1769", "locus": "GCA000209735_01767" }, { "type": "cds", "sequence": "contig_1", "start": 993658, "stop": 993846, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATAATCGTCAATATAAAGAATTAAACGAAAAATACTTCTTTGAAAGATTAAATTCATCAAATAATGCTAAAATGACTAGTTTATTAGTAGATAACTACCACAAATACTTTGTTGATTTAAATGGTAAATTACTAGGTCAAGTATCATTCAATAATGAAGATGAAAAAATTAATAAATATAGATGA", "aa": "MHNRQYKELNEKYFFERLNSSNNAKMTSLLVDNYHKYFVDLNGKLLGQVSFNNEDEKINKYR", "aa_hexdigest": "4d1107b9a47eb967b608671f251a60ad", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7550.3743, "isoelectric_point": 8.963442420959474 }, "id": "OAIKOHHJFB_1770", "locus": "GCA000209735_01768" }, { "type": "cds", "sequence": "contig_1", "start": 994024, "stop": 994197, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTTATGACATGGCTTTAGATGAAGAAATGCTTGCTATTAATGATTCAAATCAAAAATTTGATGATGGTTATAGAACAGTTATGTTAGTTGGTGGATTTGCTCAATATGTTAAAGATCATCTTAATGACAAAAATTCAATTTATGCAGCTAATAGTGGTTTACAATTATGA", "aa": "MGYDMALDEEMLAINDSNQKFDDGYRTVMLVGGFAQYVKDHLNDKNSIYAANSGLQL", "aa_hexdigest": "058cc38f27be0fd0ac50babc20ee7b04", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6392.057600000002, "isoelectric_point": 4.265277671813965 }, "id": "OAIKOHHJFB_1771", "locus": "GCA000209735_01769" }, { "type": "cds", "sequence": "contig_1", "start": 994837, "stop": 995259, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATACATTCAAACATGTATTAGATTCTAGATATTCTCTTTTAGCAAATACTCCATATACTTATTGTTTAAATATTGAAAGTTTTGTTGCTTATAAAAATCACATAATTGATGATAATGCAAAAAAACAATATAACCAAATAGTTTCTAATATTACAAATAGAATGAATTCAGCTTCAAGCAAAGAACAAGAAGCTAAAAAAATATTAGAAGAAATAAACAATAAATTACAAACGTTAAAAAATAATCCTAATACGCACTATTTTGGAAATAAAAATGTAGTAGGTCGTTCAATTGATCAAAAGCCACATATTACATTTAATATTGCTATTTATGATGAAAATCAAAAACATGATTACTACATAACAAGTACAACTAATTATCGTTTCTTCTTAAAAGATGTTAGCAAAGATATTTATTAG", "aa": "MNTFKHVLDSRYSLLANTPYTYCLNIESFVAYKNHIIDDNAKKQYNQIVSNITNRMNSASSKEQEAKKILEEINNKLQTLKNNPNTHYFGNKNVVGRSIDQKPHITFNIAIYDENQKHDYYITSTTNYRFFLKDVSKDIY", "aa_hexdigest": "2d1dd985d0c8bdff229b3ccec7af2d6d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16483.280699999992, "isoelectric_point": 9.042738533020017 }, "id": "OAIKOHHJFB_1772", "locus": "GCA000209735_01770" }, { "type": "cds", "sequence": "contig_1", "start": 995308, "stop": 995469, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5A628", "UniRef:UniRef100_A0AB32XBM8", "UniRef:UniRef50_A0AB32XBM8", "UniRef:UniRef90_A0AB32XBM8" ], "nt": "ATGCAAGAAATCTTAATTCCTCTCCACTCTCCCCTTTATTCAATTAAAGGAGAGACCTTCTTTGAAACTAAAGAAGCAAAGCTTTTAAAATTTGAAGAAAAATTATTCTTCATGGGACATCACACAAAATTTTTTACAGTCCCGGTTCACATACCATTTTAG", "aa": "MQEILIPLHSPLYSIKGETFFETKEAKLLKFEEKLFFMGHHTKFFTVPVHIPF", "aa_hexdigest": "80a69bfdd031766b0fe6329d2d4678bc", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "ups": { "uniparc_id": "UPI0001F5A628", "uniref100_id": "UniRef100_A0AB32XBM8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001F5A628", "UniRef:UniRef100_A0AB32XBM8" ] }, "ips": { "uniref100_id": "UniRef100_A0AB32XBM8", "uniref90_id": "UniRef90_A0AB32XBM8" }, "psc": { "uniref90_id": "UniRef90_A0AB32XBM8", "uniref50_id": "UniRef50_A0AB32XBM8" }, "pscc": { "uniref50_id": "UniRef50_A0AB32XBM8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB32XBM8" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6323.4245999999985, "isoelectric_point": 6.901811027526856 }, "id": "OAIKOHHJFB_1773", "locus": "GCA000209735_01771" }, { "type": "cds", "sequence": "contig_1", "start": 996290, "stop": 996496, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCCAAATTAATAGAATATGAGAAAACAATAACTGAAAAAATGCTTTTAAATATCAAAAAAATATCAGATTCTAAAATAATGCTTAAAAATGATTTTAAAGACAATTTTGTTAATTTAAATGATGCTCTTTACAAAATAAAATATGCTTTTGATTTCATTAAATACAACAATCCAACAAGCAATGGATATTATGAAGAAAGATGA", "aa": "MAKLIEYEKTITEKMLLNIKKISDSKIMLKNDFKDNFVNLNDALYKIKYAFDFIKYNNPTSNGYYEER", "aa_hexdigest": "f64bc6f4bc27e9b20bf6785b8ea22649", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8181.370100000003, "isoelectric_point": 8.802851676940918 }, "id": "OAIKOHHJFB_1774", "locus": "GCA000209735_01772" }, { "type": "cds", "sequence": "contig_1", "start": 996941, "stop": 997162, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGCAATTAAAAATAATCAAATTTCTAAATTAGATAGTCAAGGTTTAAAAGCTTTGCAACAACTTGATGGTGGTGTTGATTTTACAAACATTGATTTAATTAAAAATTATTTAAATGAAACAGATTATTTATTAGCTGTTCAAGATTTAAGAGAATATTTCTTAAACAAACTAATTTACTTATTACATGAAAAATTCAATTATGGTGATGTATACTGA", "aa": "MIAIKNNQISKLDSQGLKALQQLDGGVDFTNIDLIKNYLNETDYLLAVQDLREYFLNKLIYLLHEKFNYGDVY", "aa_hexdigest": "16de9c6bd8d0e41364b20c1cc8b94209", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8529.637399999998, "isoelectric_point": 4.791663551330567 }, "id": "OAIKOHHJFB_1775", "locus": "GCA000209735_01773" }, { "type": "cds", "sequence": "contig_1", "start": 997274, "stop": 997441, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGCAAAATTAGCAGCAATAGCAAATGTGGATTCTAAAGTATATGAATATAAAAACAAAATTTTTGATGCTGCAATTGAAGCTTATAAAAATGTTAATTCTACAGAAAACTTTAGTATTGTTCAAGCTGATAAAGACAATAAAGTTAACTTTGTTAAAAAATGA", "aa": "MDAKLAAIANVDSKVYEYKNKIFDAAIEAYKNVNSTENFSIVQADKDNKVNFVKK", "aa_hexdigest": "7b41b65147eabf9a36bdb16c44d99af6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6214.962400000002, "isoelectric_point": 7.942263221740723 }, "id": "OAIKOHHJFB_1776", "locus": "GCA000209735_01774" }, { "type": "cds", "sequence": "contig_1", "start": 998171, "stop": 998803, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8", "UniRef:UniRef90_C4XFE8" ], "nt": "ATGTCAAACATAGATAAAATAGAAGAATTATCAGCAAAAATAGAAGATGCTAATACTATTGCAAGTAATAATTCTCCAATTTTAAATCCTATTAATGCTGTTAAAAAGATTTTATTATCAAGATTTGACTCTAATAATTATGACGTTATTTTAGAAAATGGCGGTGTTTTAGTAAATAGAAGCGTTATTATAGAAGATATTATTAAGGCATGTAAAACATTAGAACAATCATATGGAAATACGAAGGAATATATAAATCGTTATGTTAATACTTTTGTTAAAACAATTGAAAATACAAAATTATTTAATAAATCTCAAATTCTAGACTTAAGCAACAAATCATTAACACCTATTATTAATGGTCTTAATGAATTTAACTCATTTGTTGAAAGTTCAAATCAAATTTCTTTTGAAAAATTAGATGAATTTATTAACAAAATTCAAGCTATTCTAAATGTTGAAAATGATTTGACAACTTCTATTCAGTCTGTTATTAAAGAATTTTTAAATGCTTATAATTCAAATGTCGATTCAATTGAAACAACAGTTAAATTAGCTAAACTAAATAACTCAAAACAAATTATTGATCAATTTGTACAAAAATCTAAAATTGAATTAAGTTCTCTCAAATGA", "aa": "MSNIDKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILENGGVLVNRSVIIEDIIKACKTLEQSYGNTKEYINRYVNTFVKTIENTKLFNKSQILDLSNKSLTPIINGLNEFNSFVESSNQISFEKLDEFINKIQAILNVENDLTTSIQSVIKEFLNAYNSNVDSIETTVKLAKLNNSKQIIDQFVQKSKIELSSLK", "aa_hexdigest": "fb7e6d4e1dd83e29bd5a97f7ba3a45f7", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 997973, "stop": 998803, "observations": { "mutation": [ 998173 ], "stop": [ 998173 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE8", "query_cov": 1.0, "subject_cov": 0.7608695652173914, "identity": 0.976, "score": 381.0, "evalue": 2.31e-132, "gene_start": 1, "gene_end": 210, "reference_start": 1, "reference_end": 210, "reference_sequence": "MSNIDKIEELSAKIEDANTIASNNSPILNPINAVKKILLSRFDSNNYDVILEKNGVSVNRSVIIEDIIKACKTLEQSYGNTKEYINRYVNTFVKTIENTKLFNKSQILDLSNKSLTPIINGLNEFNSFVESSNQISFEKLDEFINKIQAILNVENDLTTSIQSVIKEFLNAYNSNADSIETTVKLAKLNNSKQIIDEFVQKSKIELSSLKWDNYIEGRNKLINEYVNSKNELDSIKNGFKKAFVNIKSRLNEFNLTLEANKTTLNEFYLFETSYKS" }, "paralog": false, "identity": 0.9637681159420289, "score": 508.0, "evalue": 1.46e-184, "description": "Internal stop codon at 998173. Nonsense mutation around 998173.", "psc": { "uniref90_id": "UniRef90_C4XFE8", "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE8", "UniRef:UniRef50_C4XFE8" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 23671.59690000002, "isoelectric_point": 5.213408851623535 }, "id": "OAIKOHHJFB_1777", "locus": "GCA000209735_01775" }, { "type": "cds", "sequence": "contig_1", "start": 998858, "stop": 999322, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7", "UniRef:UniRef90_C4XFE7" ], "nt": "TTGTGCCAAACTCAAATTGACTCAAATGAAAAACTTATTAAAGATTTACAAGGAATTATTGATCAACACTCTTCATATCATATTGGTCACCACTTTGGTAACAAAAGTTATATAAGAGCTGTTAAACACAGTAATGAAAAGAAAATAATTCAAAATCTTTTACCAGAAATGATAGAAAGAACAAAACAAGTTATTAATCAAAGCGAATATGAAAATATCAAAAAATCAATCCAAGATTTTGTTGTTCGTAGTAATGATTTATTAACTTTAAAAACTGCAGATGAAAAATTTGTTGATGCTAATAGTAAAAGTGAATTAAATTCTTTAGAACTTAAAATTAAGGAATATCAAAACACTTTAAGTAAAATTGAAGAAGAAGCACTTTCAAGCAACATAAAACCTAACATTTCAACTGAAATGATTGATAAGGCAGCAGATTTTATTAAATCCGATTTTCATGTTTGA", "aa": "MCQTQIDSNEKLIKDLQGIIDQHSSYHIGHHFGNKSYIRAVKHSNEKKIIQNLLPEMIERTKQVINQSEYENIKKSIQDFVVRSNDLLTLKTADEKFVDANSKSELNSLELKIKEYQNTLSKIEEEALSSNIKPNISTEMIDKAADFIKSDFHV", "aa_hexdigest": "667b65428f8842532550fc40bf57fbba", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 998822, "stop": 999583, "observations": { "mutation": [ 998860, 999337, 999352, 999376, 999454 ], "stop": [ 998860, 999337, 999352, 999376, 999454 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XFE7", "query_cov": 1.0, "subject_cov": 0.6039215686274509, "identity": 0.9420000000000001, "score": 274.0, "evalue": 2.4e-91, "gene_start": 1, "gene_end": 154, "reference_start": 88, "reference_end": 241, "reference_sequence": "MKNSTKKIVALTMDISAGIVGAVSAGTLIGYAVDHSNYKKEKLWIENNIKYLKESNQKLKEKDNKLKEIWKKYENQEWLSSLWNELSLCQTQIDSNEKLIKDLQGIIDQHSSYHVGRRFGNKSYMRAVKHSNEKKIIQNLLPEMVERTKQVINQSEYENIKKSIQDFVVRSNDLLTLKTADEKFVDANSKSELNSLELKIKDYQNTLSKIEEEALSSNTKPNISTEMINKAADFIKSDFHVWNDKIIKKYVKKTA" }, "paralog": false, "identity": 0.9090909090909091, "score": 437.0, "evalue": 8.08e-158, "description": "Internal stop codon at 998860, 999337, 999352, 999376, 999454. Nonsense mutation around 998860, 999337, 999352, 999376, 999454.", "psc": { "uniref90_id": "UniRef90_C4XFE7", "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XFE7", "UniRef:UniRef50_C4XFE7" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFE7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17763.89219999999, "isoelectric_point": 6.07150707244873 }, "id": "OAIKOHHJFB_1778", "locus": "GCA000209735_01776" }, { "type": "cds", "sequence": "contig_1", "start": 999452, "stop": 999583, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAGCACAAAAAAAATTGTAGCTTTAACAATGGGTATTTCTGCCGGAATTGTTGGTGCAGTATCAGCTGGTACTTTAATTGGTTATGCAGTTGACCATTCAAACTATAAAAAAGAAAAATTGTGA", "aa": "MKNSTKKIVALTMGISAGIVGAVSAGTLIGYAVDHSNYKKEKL", "aa_hexdigest": "28888bcde023630159e6cd6262b36ece", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4466.227499999999, "isoelectric_point": 9.778580665588379 }, "id": "OAIKOHHJFB_1779", "locus": "GCA000209735_01777" }, { "type": "cds", "sequence": "contig_1", "start": 999951, "stop": 1000166, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGTTAAAAATGAACTTCTATATATAAAAGAAGAAAAAGGCAAAAGACAAGCTATACCTGAAAAGGAATTAATTAAACTTGTTGAAGAATTAAAAAGCTACAAAAACACCAAATTTAGTATCATTTTTCGTATTTTTCTTTCAACTGGTATTAGAGTTGGAGAATATAAAGAATTTATTAAAAACATTGATAAATTAGAAAAAGAGAATTGA", "aa": "MDVKNELLYIKEEKGKRQAIPEKELIKLVEELKSYKNTKFSIIFRIFLSTGIRVGEYKEFIKNIDKLEKEN", "aa_hexdigest": "c32fee22b7da91596b9956dae8297d54", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8507.892799999994, "isoelectric_point": 9.155622673034667 }, "id": "OAIKOHHJFB_1780", "locus": "GCA000209735_01778" }, { "type": "cds", "sequence": "contig_1", "start": 1000233, "stop": 1000688, "strand": "+", "frame": 3, "gene": null, "product": "Tyr recombinase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG84", "UniRef:UniRef90_C4XG84" ], "nt": "ATGCCTTTAATAGGTAATCTTAGAGAAGATATATTAGAAAATATAGATAAATTACGTATTTCTGAAAAATCAATTAAAAATATGTTTTATCTTTTTAAAAAACATATTAGTGAAAAATATCCTGATTTTAAATATAAAATCTCGGCTCACGTCTTAAGACATAATTATGCAACTAAAGCTTTTTATAATTTAAAAAATAATGATTTAGTAGCCAAATTATTAGGACACACAAATTCAAATATTACTCAAAAAACTTATATTGATTATGATAAAAGTATCATGGATGAGTATGCTATTTTAGCCATACAAGATCAAAAAATAGGCTTAAATTCTATGCAATTATTAAATATAAATAATAATTTAGAGCTTACAATTATCAAATTAGAGAAAGAAAATTGCAGCTTAAAAGAAGAAATTGAGATGCTAAAAAATAAAATAAAAAAACTAAAAAAATAA", "aa": "MPLIGNLREDILENIDKLRISEKSIKNMFYLFKKHISEKYPDFKYKISAHVLRHNYATKAFYNLKNNDLVAKLLGHTNSNITQKTYIDYDKSIMDEYAILAIQDQKIGLNSMQLLNINNNLELTIIKLEKENCSLKEEIEMLKNKIKKLKK", "aa_hexdigest": "fa9748b6dc4e581eea21cfd8ec3835b9", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 999933, "stop": 1000688, "observations": { "mutation": [ 1000164 ], "stop": [ 1000164 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_C4XG84", "query_cov": 1.0, "subject_cov": 0.4326647564469914, "identity": 1.0, "score": 288.0, "evalue": 2.17e-95, "gene_start": 1, "gene_end": 151, "reference_start": 199, "reference_end": 349, "reference_sequence": "MCLYIKIFIRHFWRSKNMKKNNNFLDIFLNNIYSMVQKNQRSYQTFKQYKRYLSSLKNWNNAKQLIVRMNRELANNKISNNTKLTKRNIFSSFLNWFCKITNTNMDVKNELLYIKEEKGKRQAIPEKELIKLVEELKSYKNTKFSIIFRIFLSTGIRVGEYKEFIKNIDKLEKENWSFIIKTLKNNNPRYFRIPSKLEMPLIGNLREDILENIDKLRISEKSIKNMFYLFKKHISEKYPDFKYKISAHVLRHNYATKAFYNLKNNDLVAKLLGHTNSNITQKTYIDYDKSIMDEYAILAIQDQKIGLNSMQLLNINNNLELTIIKLEKENCSLKEEIEMLKNKIKKLKK" }, "paralog": false, "identity": 0.9960159362549801, "score": 490.0, "evalue": 1.52e-177, "description": "Internal stop codon at 1000164. Nonsense mutation around 1000164.", "psc": { "uniref90_id": "UniRef90_C4XG84", "product": "Tyr recombinase domain-containing protein", "uniref50_id": "UniRef50_C4XG84", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_C4XG84", "UniRef:UniRef50_C4XG84" ] }, "pscc": { "uniref50_id": "UniRef50_C4XG84", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XG84" ], "product": "Tyr recombinase domain-containing protein" } }, "id": "OAIKOHHJFB_1781", "locus": "GCA000209735_01779" }, { "type": "cds", "sequence": "contig_1", "start": 1000988, "stop": 1001119, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAGCACAAAAAAAATTGTAGCTTTAACAATGGGTATTTCTGCTGGGGTTGTTGGCGCAGTATCAGCCGGTACTTTAATTGGTTATGCAGTTGACCATTCAAACTATAAAAAAGAAAAATTGTGA", "aa": "MKNSTKKIVALTMGISAGVVGAVSAGTLIGYAVDHSNYKKEKL", "aa_hexdigest": "642402a76e340460a2aa707beead737e", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4452.200899999999, "isoelectric_point": 9.778580665588379 }, "id": "OAIKOHHJFB_1782", "locus": "GCA000209735_01780" }, { "type": "gap", "sequence": "contig_1", "start": 1001406, "stop": 1001505, "strand": ".", "length": 100, "id": "OAIKOHHJFB_1815" }, { "type": "cds", "sequence": "contig_1", "start": 1002257, "stop": 1002421, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAATTTAAGTGAAAAATTAAAAAATTGTAGTGAGCAAGAATTAAAAGATTTCAATTATTTTATAGATGGTATGTTAGAAATGCCATTAATAAATGCTATTGATGAAGTAATTGATTGTCTTGAAAAACCTGATTATTGTGAACATTATGATACTCATTGA", "aa": "MSNLSEKLKNCSEQELKDFNYFIDGMLEMPLINAIDEVIDCLEKPDYCEHYDTH", "aa_hexdigest": "cf8540ac23419744ba1c09e0b3a718ca", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6386.1314, "isoelectric_point": 4.215486717224121 }, "id": "OAIKOHHJFB_1783", "locus": "GCA000209735_01781" }, { "type": "gap", "sequence": "contig_1", "start": 1002780, "stop": 1002879, "strand": ".", "length": 100, "id": "OAIKOHHJFB_1816" }, { "type": "cds", "sequence": "contig_1", "start": 1002949, "stop": 1003218, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGCTTCTAATGCTAAATTAAATTCATTTAATCTTGATTTAATATTAACAAATGCTTTTTTAAATCCACTTTTTATTGAATCTAATTCATTTTTGCTATTAACATATTCATTAATTAATTTGTTTCTGCTTTCAATATACTCATCTCATTTGAGAGAATTTAATTCAATTTTAGATTTTTGTACAAATTCATCAATAATTTGTTTTGAGTTATTTAGTTTAGCTAATCTAATTGTTGTTTCGATTGAATCAACATTTGAATTATAA", "aa": "MFASNAKLNSFNLDLILTNAFLNPLFIESNSFLLLTYSLINLFLLSIYSSHLREFNSILDFCTNSSIICFELFSLANLIVVSIESTFEL", "aa_hexdigest": "ecdcb3573624772f90080294155e0366", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10110.622799999988, "isoelectric_point": 4.246577644348145 }, "id": "OAIKOHHJFB_1784", "locus": "GCA000209735_01782" }, { "type": "cds", "sequence": "contig_1", "start": 1003393, "stop": 1003707, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAAATTTATTTTTTCAAATAGTCCTTTAAATTTTTCCTTGTCATTTCGAATTTCACTGTTTTCAATTGAAGATATTTCTTCAGTTGATAAATCAGGTTTGTTATTATCAATATCTTCAATTGTTTTTTTAACATCTTCAAATTGGCTTAATGTTTTTAAATTAGCAAAAACTTTTGATAAGAAATCAACAATTTTTGGATTTGCATTACTAATAAGTTTTGAAATTTCTTTAGGTTTTGAAGCAAAGTTATTAACTTTTTGTAAGTTTAAATCAGTTGCGTTTTTAATAATTTTAAATATTTCTAAATAA", "aa": "MIKFIFSNSPLNFSLSFRISLFSIEDISSVDKSGLLLSISSIVFLTSSNWLNVFKLAKTFDKKSTIFGFALLISFEISLGFEAKLLTFCKFKSVAFLIILNISK", "aa_hexdigest": "6036e17d51597afc3f4d971a728c2a35", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11676.756399999982, "isoelectric_point": 9.667566108703614 }, "id": "OAIKOHHJFB_1785", "locus": "GCA000209735_01783" }, { "type": "gap", "sequence": "contig_1", "start": 1003861, "stop": 1003960, "strand": ".", "length": 100, "id": "OAIKOHHJFB_1817" } ], "sequences": [ { "id": "contig_1", "description": "[organism=Mycoplasmopsis fermentans] [strain=PG18] [gcode=11] [topology=linear]", "nt": "ATTTCTCTTTTTATTCGGATTCTTTTTTCGTTTTTTCCATTTTATCTTAATCTACCGCCCCCTTTTCTTTGCACCCCATTTTTTCCCTGTCTGTTTTTCCGTGTTCACTCATTTTCATTTTTTTTAAAAACTCCTACCTAGCTGAGTAGGTCTTTAGTTGTCGGTTTCCCTCTTCTTTTCTCCNATCCACTGTCCTCGACGCATCGTTTTGCCTTCGTTCCCTTCCTTTTCTCTCTTCAAGCCCATTCTCGNTTTCGTTATCCCCATCCTCTCGCCTCTTCCTTTCCCAANAATCGTCCGGTTCACTTGATTTGCTTGTTCTCCTCTCTTTCGTACCGGGCGTTACTCTTTTGTTCTCCTTTTAATTATGCGGATCCATAATCGCTTTCCGCTGGACCCCTTGTTTTCCTACTGAAGTNTGGTCCACTCGTGTATGTGGATTCGTACAATGACTCCTTGGCCCCGTGTGGTGTAATTNTATAACAACACATGGTATNGATATTGCAAAAATGGTGTATATCATAAAGCCTATTTTTCCTTTTATTGCAATCAATATTATTAAGGATAATTCTAATAATAAAATAATTGTATTAAGAATAATGAAACCAAACCATATTCTCTCTTATTTTTAATGCTCATAATTTCTCCTTACTTAGATTTTTATAAAAAATAAAATAACATCATTAAAGACTTAAAAACGTAATAATAGAAATGCTATTTAGTTTTAAAAAAACAAAAGAAAATAATGAATAAAAAATGCAGAAAACAAAGAATGAAAATTTTTATTATTTTTAAACAAAAATATTTTTTATTATTATAATTATTCTCATTATGAGAATACAAAATTTTAAGCGTGATAAATTTAAATCTAAATTTGTATTATTAAACTTTTGCGAAGATAAAAATCCAGATCTTCCATTTTTTGTTATTGAGTATTCTCAAAACGTGTTTGATTTAGCCTCCCTAAGAAGTCAATCGATTTAATTAAATTTAGTTAACTTTGATTTGACGCCAAAATAAAATATTTTAAATTTATTTATTACCAAAAACGCTATAAAACAGATTATTTAGAAAAAATTTATATTTTATTTTTTAGGAGAATTTATGATTAGGAAAAATAATACACCTTATATTATTGAATTAAAAGAAGTAGTTAAAGAATTCGATGATAAGGTTGTTTTGGACAATGTTGATTTAAAAATCAATAAAGGTGAATTTGTTACTTTGCTAGGACCTTCAGGATCTGGCAAAACTACAATCCTTAGACTTATTGCTGGTTTTGAAAGAGCAACACGTGGTGAAATTAAATTTCATGGTGTAGATATTAAAGACTTGCCGCCTCACAAAAGAGACCTTTCAACTATTTTTCAAGATTATGCATTATTTCCTCACTTAGATGTTGAAGGAAACATTAAATATGGTTTAGCTCTTAAAAGAAAACCAAAAGAAGTTATTAATGCTAAACATGAAAAATTATTAGTTCAAAAACAAAAACAATGAGAAGAAAAAGCTCAAGCTGAAATGAAAAAATTAGATGCTCTACAAGAAAAATATGAAAGAGAAATGGAAACATTAAAACCAAATACTTTTGCATATCGTCGTAGACAAAAATGATTAGATAAATCAGACTTCAATTACTCATATTGAGAAAACTATGTTGAACAACAAACTGAAAATTTTGAAAAACGTTATTTAACTAGAAGAATAACAAAAGAAGAAATGGACCAAGAAGTTAAAGAAATTGTAGAATTAGTCGGACTTCAAGGTAATGAAAAAAGATCTATTAATGAACTTTCGGGTGGTATGAAACAACGTGTTGCTTTAGCCAGATCTCTTGTTATTGAACCTGCTATTCTTTTATTAGATGAACCTCTTAGTGCTCTTGATGCCAAAATTAGACAAAAAATGCAAATATTATTAAGAAGCTTACAACAAAAATTAGGTCTTACATTTATTTTTGTTACACATGATCAAGATGAAGCTCTTGAACTTTCAGATAGAATTGCAATTATGCGTGGTGGAAAAATTGAACAATATGACACGCCAAAAAATATTTATGACTATCCAGTTAATAAATGAGTTGCATCATTCGTTGGAGACTCAAATATTTACAATGGAATCTTTAATGAAGATGGCAGCGTTACAATGTGAGAACGTAAATTTAAAACCATTCATGATGAAGATGAATTTCCAAACCAAACTGAAGTTGATGTTTTAATTAGACCAGAAGACATCGATATCATGGCTTATGATGAAAAGAAAAAAGACAAGCTTGTTGGTGAAATTATTAACATGGCTTACCGTGGAAGTTATTACTACTTAAAAATTCAACTTGACAATGATGAAATCATTTATGTTGAAACAGCTAAAAAATTCGAAATAGGTGAAAAAGTAAACTTAAGCTGAACAATTGACTCAATTCATTTAATGAATAAAGATAGCAAATGGGATTACAAAACAAATGAATTCAAAAATTAAAAACAAACTTGGTTTAAATAAAAGATTACTACTTTTATTACCTTATTTATTTATTGCTATCTTTTTAATAATTTTGCCAATTTTATTAATCGTAATTAATGCTTTAAAACCACAAGATGGCTTTAATGCATCAGCTTTATTAGCTGAAAAAGGAATTTGATTAATAATTTGACGTAGTTTAAAAATTGGATTAATTTCAGCAATATTATGTTTAATTATTGCTTTTCCATATGCTTATTTTATTGCTACAACTAAGAGCAAATATTTAGCAATTTATGCTTTAAGTTTAATTCTTAGTCCTATGATAATTTTTACAATTGCTAAAATTTATGCAATTAGAGGTTTCTTCCTTTCAATTTTTGAAGAAGACAGTTTGAATGCTGAATGATTTATGGTTTTTGGTTTAACTTATTTAAACTTGCCATACATGATTATGCCACTTTACACAGTCTTTAAAGACATGCCTAAAAACATTCTTGAAGCTAGTGCAGACTTAGGTTACAACAAATTTCAAACATTATTTAAAGTAGTTGTTCCATATAGTTTCAAAGCTATTTTAAGTGGTTTCAGTATGATCTTCTTATCATCAGCAACCACCTTTGTTATTAGTGCAAAATTACTTCCAAATGGTACTCAATTACAAACTATTGGATCTGTAATTAATGATTATTCAAGCCCTTCAAACAAATATGAATTAGCTTTAGGAAGCACACTTGTTTTAGTTGTATCAGCCATATTTATAGGTTGTTACGGATTAATCAATTTTGTTCCTAAAATTATAATTAGACTTAAAAAAAACTAAAGGAGGTAAGAAACATGACTAAATTTTGAAACTTTATTAAAAGAAGTTACATTTACATTATTTTAGCTGTAACTTATATTCCTTTAGTGTTCGCAGTTATCTTTAGTTTTAATAAGCCAAGTGATAAAGGTTTCTTGAGCTTTTCATGAAATGGTTTTACTAATACTGCTTGAACAAACTTTTTTGATGAAGGTAGAGAACTAGCTTTAATTAACTCAATTATTATTGCTTTCTTTGTATCTTTATTAGTTATTAGTATTTCACTTGTGACAGTGTTTGCAATGTGAAAACAAAAAAATAAAAACTACTCAAGAGCAGTTAGAGCTGTTAATAATATACCCTTTATTAACCCAGATAACATTACTGCTATTGGTTTAGTTTTAGTATTCACAGCACTTTTCGGAACTTTAGCTAACACTAAAGAAGGAATGATTAGAGGAATAGTAGGTCACACAATTATGGCTTTACCTTATGGTATTACTTTAATGTATCCAAGAAGTGAAAAATTCAATGCTTCATTATTTGAAGCGAGCCAAGATTTAGGTTATTCAAAAATTAGATCATGATTCAAAACTTATTTTGTTTACATGATTCCATCAATTGTTTTTGCTGGTTTAGTTTCCATTTTCTTATCATTTGATGACTTTATTATTTTACAAACAACTTCAAATGTAAGTACACTTGGAACCAAACTTTATGAAGGTCAATTTAAAGCTTGAGGCTTAGTAGTTGGAGCTTGCTTATTATTTGTTACTATTGTTGCTAATGCTTGCTATATTGGTTATAAAGCTTACATGATTAAAAGAAGTAATAAAAAAGCAAAAAGGATAAAAAATGAACAACAACAAACCAAAGAACAACAAATTTAAAACACTTATTAAAACCAAAATTTTAACCAAAACTTTTGGTACAAGTGCTCTTGCAGTTGTTGCTGCAGCTGCTTTAGTTGGTGCTTTATCTTATAAATATAGTAACAAAAATAACTTCAAGCCTTCATTCTACAACTATCAATCTTATATTGATCCAAATACCAAAGCAAAAATAAATAAAAACTTCAACTTCAAAGAATTTGCTGAAATTCAAGATTTTACTAAAGCTATTTTAACTAATAAAGCAGCTGCCGGAATTGGCAACGATTCTCAAGCAGTTCAACTTATTAGAGAGGGTAAACTTCGCAAAATTGATTATGCTAAAATGTTTGGTGATCCTAAATTTAATGACCGCAATGAAGTTTTAAAACTTTTAACTGACACAGTAAAAAATCATCTTGAGTCATATAACAAATATTTAACTAAAGATAAATGTATTAATGCCGATGGTTCGCAAGCTACTGAGGATCGTCACTTAATTGACTACTTTGCTCCATACTTTTCACAAGATATGGTAGTAGCTTATAATCCTGCTAAAGTTTTAAATCTTAAAAAAGATGACCCTGAATATCAAAATAAAATCAATGCTCATCAACAAGCAATGCAAAATCACCTAATTAATGAAATAAAAAACAACAAAGGTGAATTTAGACTCATTGATATTCTTAAAACATTAAAAGCTAACAAATATGAACATTGAGAAGCAACAAATGCTGTAAGAGATAATATGATTTATGGTTCAGCTTATGTTTATGACGTTAATAAAGGCTTTGTTGATGATCACGCTACTGGAAAAGGCACATCAAAAGATAAGCCTAATTGATATAAAGAACACATTGACCACTTTGTTGAATTATTTAATCATGGTATAGGTTATAGTGTTAGAGATACAAAACATATTAACTTTAATGGAGATGGACAATTTCTTTTAAATAACTTAATTAATCCTGATTCGAAAACATGTGCTGGTTTAATTTATAACGGTGATGCAGTTGATGCTTATTTCTCAGAAGATAACTATGCCACAGTGCCTCAAAGTACTATTAGATTCTTAAGACCAAAAGTTAATTTATTATTAGTTGATGGGTTAGTTATCTCAGAAAATACAAGTGATTATTTTACTGATTTAATTTATAAAACAGTTCACAAGAGCTTTCTTGGTGGAGTTGAAAAAGATAAATGAATCAATGGTCCAAAATGAAATAGTACTACTGAAGAATGAAATAATATAGAAGGTTATGAATCATTTACAACATTTGACTTTGTAGGTTATACTCCTGCTTGAAAATCACAATATGAATTTGTTAAAAATAACTATTTTGTTGATGATACATATCCACAATATATTGCTAGTCTTTATGGAATAAATAAAGAACAAACTATTTTTGATGTTGATAATACAACACCAAAAATGAAATATGAAGTTAAACATTTTCCTATTGAACCAGAAAGTAAACAAACAATGGCTGAAATCAAAGTTTACTATGATACAAAACTAAAAAGTTAATTAAATTTAAGATTCTTAGATGCAAAATCTAAGGATTTTTTATGCTCTTTTTTAAGAAAAAGTAAAGATTTTGTTTAAAAAGTACGGAAAAAAGTAAAATATAATTAAAATTATTATTAAGTAGGTCCTATGTTAAAAAGAAAAATAGATGCTTTTTTGTTGGATTGAAAAAATGATGCCAATAAAAAACCATTAATAATTAAAGGAGCAAGACAAATAGGCAAGACAACTTCTATTTTAGAATTTGCTAAAAATAATTACAAATATGTAGTTTATATCAACTTTGCTTTAATGAATGAAATAAAAGAATTGTTTAATAACAAAAATGTTAAATCTTATGAAGTTGATGATTTAATTAAATTTATTTCTTTTGCAAAAAGCGGTTTTGAATTTGTGCCAAATGAAACTTTAATATTTTTTGATGAAATTCAAGAACAACCTAATTGCATTACAAGTTTAAAAAGTTTTGCCTTAGACAAAAGATTTGATGTTATTTGCTCAGGTTTTTTATTAGGAATTAATTTTAAAGAACACAATATTAGACACATTGGAGTCGGTTATATTTCTTAGTATGAAATGAAACCTATGGATTTTGAAGAATACCTTTGAGCAAAAGGATATAAAGATGAAGATATTAATTATCTTTTTATATAACACCTAAATAGTATTCTAATAAATAGTCAAGACCTGGTATTAGCCAGGTTTTTTTGTTCAAATAGGTGAATGAGTTTACTTTTCAAAATACTTTTATTTTAGGAAAATTCTTTTATATTTAAACTTAACTTAAAATTATTTATTATCTTACTTCATATTGTATGTGGTTTATATAAATGTATTATACATTTATATCAAACCACCACACCAACTAAGGAAATCAAATCAATAATTTTTTCCTATGCTTTAACCTATACACCGAATTTTCCTAAAGAAACAGGGAATATATAGTGATATACTTCCCAAAATTAAAATTTCAGGACTATATATACCTCCCCCTTTGAAAAAAAAGACCCACCGGAGTTTTTAATGCCCTTGTTATTTTAGAAAGGAAAAATATATGCTACTCACTACAAAACAGTTACAGGAATTTTACAAAATTGAAATGGAAAATATTGCAAAAAATAAATCTCTATTAACTAACTTCAATATCATTGATAATTATGCAAATGGTTTAAAACCTAAACAACTTTATTTTTTAGCTGCAAGACCAGGCGTTGGAAAAACTTCTTTTGCTCTTAACATTATTTTTAATGCTTTGGAAAATTTGAAAGAAGATGAATGCATTGTTTTGTTTAGTCTTGAAATGGACTCAATTGAAATATATTCAAAACTTTTAGCTCTTGCCAATTCTTGTTCACTTAAAGAAGTTGAAACAAATTCTAAGAAAATAGATTTTGAAGATTTAAACAAAAAGAACCTTTTTATATTTGATAGATATAGAAACAATTTTAATAATGAAAAATTAAGTGAAGTGACTCCTGACATTATTTATAAAAGTTTGAATTCTATTGGTTTAAAGATCAAAGCTGTTTTTATTGATTATTTTCAATTGCTTGATAATCAAAAATATTCAAGTGAAAGAGAAAAGTTGTCTCAATGCTCTAAACAATTAAAAGAATTGTCAAAAATATTTAATTGTAATTTTTTTGTTTTATCTCAACTTTCAAGAGAGTATGAAAAGAAAACCAATAATGAACCATCTTTTTCTGATTTAAAAGGAACTGGTTCTATTGAACAAGACGCTGATTTAATTATGTTTCTTTATAATTCTTCAAATAATTTTAATGTTGGCACTTTAATCCCTAACAAGAAAACAATTTTGAACTTATCAATTGCAAAAAATAGAAATGGTGAATTGGTCAAAACAAATATTGATTTTATTCCGCATTTAGCAAAATTTATAGAAAGTTAATTATAATTATGTTAAATAATAAGTGTGATTTTTGCCACAAAAGATTTCAACCAAAAAAAGAAAGATATTTTTGAGGCAATTTAGTAGTATGTTCAGAAGAATGCTTAGAACAAGTTTCTTGTTATGCAAGTGAAATTGATTTAAATTGTGAGGGAGAAAATAATGAATAAAGAAAAACTGATTAAATTTCTTATTGATTTAACAGAAAAAGAAAATACAAAATTAAAATCAGATGTTAAAATTCTAAAATCTAAACTTAAAAAAATAAAAAAAGAATATGAAGAACAAGAAAAAGCTTTTGATGACTTATTAAAAAAACTTTAGTTTATGAATAAAAATAAAAAAATAACCATATGAGCTTTGTATGATGACGGCAATATGAGTTATTTTAAAGCATTACAAAACAATGAAAATTGTGTTATTTATTCTATAGGAATACAAGAACATAAAGATTTAAAGAATTATTATAATATTGACCTTTCATTAAATAATTTTAATTTAATTGACCAACTAAAACTAATTCCAAAACCTGACATTATAATTGCTTCTCCACCTTGTGAGAGTTGAAGTAGAGCAGACTGTTCACTAGCTATTTGAAAAGATATTAGCAAAACAAGTTGAGAATTGAATAACTATGAATTTTATAAAACAGTAGAAACCACAAAAAACAAAAAAAGAGATTTTTACTCTAAAGAACAAAAAAGAGTATTAGGTGAAAGTACTGCTGGAGCGACGGCTTATATAATTAATGTCTTTCAACCTAAAGTGTGAATAATTGAGAATCCTTTTCAGTCTTTTATTTGAAAATTTCTGAAATTTCATCATAACTTGAATGGAATTAAAAATAATACCTATTACTCAAGCTATAACAAAGAATTTAGTGTTAAGCCAACTTGTTTTTATTCAAATATTAATTTAAATTTGAAAAACAAAAGAAAACCAGGCAATGCTGAACACTATTCAAAAGGTAGTTATTATCAACGCTCAATGATTCCAAAAGAATTAATTAAAGATTTATTTTTAAATGTTTTTGTCCAATTGAAAAAAATTTCTGTATTAAGTAAAGAATATACAAAAAACTATATTTCAAATTCACTAGAGAAAGATCAGATCTCCTTATTTGATTAAAAGGGGATTATAGGGGTTATTTATATAAGTATAAAAATACTTATATTAGAGAGATATATATGAGCTACACTCATTATATATATCTCTCTCTAAGAATATAAGAATGCATTACATACTTAGAAAGGAAAAGAATGGATATTTTTAAAAAAATGGAAATTGCTGAAAAAGATAAAATTGAGAAATTATTACTCAACATAGATAAATTCATTTGAGATTGTAATTCCAAAGGTTGAACTAAATCAAAAAACAAAAAAGTAATTTCTTTATACAAAAAAACTCTTGAAGAAATTAAAAAAAATGAAAAATATGATTGGCTATATGTTCCTATTAAATCACAAATCTATAAATTTGCAGATCATGATTTAAAGTCTGACATATTTTTGTGATTTAGTCACACCAAAATTTTATGTGGCTATACATATTTGAAAGATAAATGAAATTTCTAAAAGAACCTCCTGTTTTTACAGAAGAACAATTTAAAGAATTAGTTCATTATACTGATTTAGAAGATAATCCAACTTTTGAAGCTTACAAATCTAATGCATTTTTCAAAATCTATTCAGATGCTAAAGGTGTTTCAAAATCTTTTAGTAGAATGATTGAAACTGTTTATAGATTAGTGAATGAAAAAAATTTTAATAGTTTTTGGTGTAGAAATCAATATAACCACATTAAAGGAACATTAAAACCATTATTAGAAAAAGTTTTAAGTTTTCTTGCTGCTAAAGGTTTAGATTATAGACCTTATATTAGTATTTACAATACTGAAGCTTATTGAGATTATGAAGATGGTGGAAAAGGAAGAGCTATATTTTTTGGTAATTGAAAAAATGTTCAAAGTTTCCAAGGTGTAACTCTTTCACAAAAAGATTTTGCATGGGGTGAATTAGTAATAGATGAACCAATTGAAAAAGAGCTAACTGAAGAATTAGAAGAAATTTATAAAATTCAAGCAAGTAATTTAGAAATATTAATAGCTAATACTGTTTTAAGATCTAAAAATATTGAAGGGTTTAAAACTAATGTAACTTTTCTTTATAACATTTTCACAGTAGATCACTTTTTAATTAAAGATTTTCATAATCCTATTTTACCTTTGTATTCAGGAACAGATTTTTTAAAGAAGATCAATTTAAATTTAGCAAAAGAATTAATTGAAAATACTTATTTGCAAAAAGAAGACTTAGCTTTTAAAGAGGGAATCGGAATTATTGTCACAATGTTTTCTAAATATTTTGTTCCTAAAAATGAATTAAGTGATTTGCAATTAAAACAATTTGAAGCTCTAAAAACTAAAGATTATAAATTATGAATGATTACTGTTGCTGGTTTTGCTTTTGAAGAGCCACCTGAAAGATATGATTATTTTATGAAAAGTGTTTTGCTCAACAAAAATGGCAATTGAGATAGAAAAAAATGCAAAATAATCAATATTAAACAATTAGAAACAAAATTAGAAAATGAAGAAATTTGTGGACTCTTTTATGGTTTTGATTATGGTTTAACTGATAATTGTGCTCTTGTTGTTGTTTTGTTGTTAAGTGGAGCAAGAATAATAGTATTAGACATTTTTGAAGACATTAAAAAGCTCTTACCAAAAAACAAGAGAAGAGAAAATAAAGCTATTTACAATAAAGTTGCTTTAATTGTCAAAAAATGAAATGAGTATTTTGATAAATACAATTTTAAATTCTATAACTCATGATTATTAGGCAATTTTGATTCTTTCTTATATGGAGATAATGTCCACTCTTTGCAATGAATGACTGAAGTTTTTATTAACAATGGGATAAATACTAAATTGTTACCTGCTTCAAGATTTAAAGGCAAAAAAGGCTTTGGAATAATTGACAGACAAGTATGACAAAAAAACATATTTGAAAATGGTTTAGTTGAGTTATTACCTAAAGCTAAATCATTATTAACTCTGTTATGTCAACAAGTAATTGATAAAGAAGATCCTAAAAATCCTAACCAAAGAAATGAAAGAATTAACAAAAAAATTTATGATGTCATCAATGCTTTTGAAATGGCCAACACCTTACAAAATTGTGAATATAAAAACTATTTATTTAATAAAGAATTAAAGGAGAGAAATGAGCAAAACAATTATTAATAAATTAGAAAATGAAAGCAACAAATATTTTAGTTTAAGGTTTAATAATAAACCTGATTCATATAATCAATTTTTAAAATTTGGAACTCAAACAACTATTAACACTATTTGAAGCAATTTACCAAAAATTTTTAAGAATAATGTAGAAGTTAAAGACTATTTTACAATTTCAAAATTGCAAGCTTTGCAAAAATTAGAGCAATATCTTTATTGTGAGGGAGTTGCTGCTCTAAGAAATAATTGAATGCCTGCTCAAGTTTTAGATTATTTGCTTGATAGAAATGGTAACTTGGTAAAACTTAAAACAAGAATCAAATTTTATTCATCAAATATAAGTTTCTATATTGATGAAGAGTGAATAAGAATTAAAAATAAAGTTGAATTTAAATATGATTTACTTTTTGATCCTATAAAAAATGTTCTATTAAATGTTGAACAAGAAAAACAAGCTAAAGCTTTATTAGATTTTTATGACTATGAAACTCTTCCATTTTATAACCAAATTCCTTATCAAATTTTCTTTAATAATCATAATGCTTTCCCCGATTTGTTTAATGTTAATTTATATTATTTTGATATGCTAGATGAAGACTTAAAAATTGCTGCAAATGATTCAAACTTATCTGCTCCTTGAATTTATGTCAATGATATGAATGGTCTTAAAGACATGATCAAACAAGGACTTAAAGATCCTAATGATAGATTTATTCCAATTAATACTGCTATTCAAATGGATGGAACTCCTCCTGTTCAACTTGTGCAAGGCAATAGTCAAGCAATACCATTAATTGAAAAAATAAGATTCAATATTAAACAAATTAAAAAATTTGCTTTCTTAAAAGAAGATTCAAGTGATTCTGGAACTAAAAATATGCATAATGCTGAAGTTCAACAAATTAATAGTGATTTTGAAGATCATATTGAAGCAAAATCAAACTTAAGACAAATGCAACTATTAAACTTTTTCAAAACTTTTGAACCTAGTTTGCTAATTGATAAAGTTTTAATTTTTGGTTCAACTGAATGGCTAAAAAATGAAGCTTGAAAATATTCTGCAAATTTAAATGGCTCAATTATAAATCAAACACCTTTAATCAATAATAACTCAAAAGCTTTAGAAGAAAGTGAGGAATTATAAAATGGACTTTATGGAATTAAAATCTTTTTTAGAGCCAAGAACTGGTGAATATATTTTAATAGACAATAAATCACTTTCTTTTGAAAATGGTTCAGTTAATGTAATATCTTGAACTAAAGACAAAATCAAAAACATTGAATTATATTGTTCTGTTGAATCTCAAAATAGAATTGATATTGAAAAAGATATTTATGATGTCATAAAGTTAAGCATTGATTTCGAACAACCTATTTTTAAATATAATCAGAGTGCAATTTTGAAACTAGATAATAAATTTTATATTTTAGATGCTTTAAATAACCAATATGATTTGGAGCAAAAAAATAATGCTTATTCATATGGAATAGCTCAACCAATTTCTCAAATTGAAAATGAGAAAAAAATAAAATTTGTTTTCATTGAAGAAAAGCCAAATGAAATTAATGTCAGTTTAAGAACTCCATATTTGCACCAAAAAGCTTTAGAAATTGAAAACAATGGTTTAACTAGTGAATTGCCAACAAATGGCCTAGCTTTTGATATGTTTGTTAATCCAATTGAAATTAATGGTATTGACAATAAATACTATTTAAATACTTTGAGCAAATTTTCTCATTTATATTTGAATATTATTTTGAACAAAGAAAATGATTTAATGAAAAACTTTTGAGGTGCTATTAATCAAATTGATAATGTTAGACTTAGATTACAAAATAATTTCATGGGAATGACTGAAGAAGAAATAAAAAAATTTAAAGAGACTATTTTTGCTGGAAACAATTTAAAAGATTTTGTTGATTTTAAATTTTACACTTTGATTAACTTATATACAAAGAAAGAAAAATTTTTAAAAACTTTATTTGCAATGAGTTCAGGCAATGTTCCTTCAACATATTGTATTGATAATGGTGAAACTGAAAAACATGAACTTTATTTGCCTTTTTATTTTGATAAAAATTGAGAAATAGCAAGTAATTTTTATGATTTAGTTTATGAAAATAATTTGATAAAAAGCATAAAACCAAAAGCAATAAAAAGTCCAAATAAGCTTTATCAAATATCTGAACCACTAATTCCATTAAATATTCAAGAAGTCAATATTTCTGATAATTGAAAAGACTTTGATTTTAATAATTCAAAATATAAAGTCAAAATGTTTATTGACCAAAAACATGAAGAATTTTTAATGTTTAATAAAGAAAAATTTAATGAAGCTTTTCAATTATCATCAATTCAAACTTCTAGATATACTATGACACCAGAAGAAGCTAGAAAAGAAATTAAAGACTTAGAACCTGGTTTAACTAAAAAGCAAATGTTTGTTCAATTAATTAAAAAAAGCGTTAAAAAAGAAATAAACATTGTTAACGCTTTTGCAAAGGTTCAATTAAACCCAACATATACAGCTAAAATTGCACATGGTGATGAACCTGGAATGATTCATTATACTTATGACACAATTCCTGCTGCATTTAAAATTGTTGATTTAGATGATTTAAATGATTGGTTAGAAGTTGTTAATTTTTCAGGTCTTGATTACAAAAATCTTGACGATAACTATGAATTAATAAATGAAACTTATGTTCCTATTTATACAATTGAATATCAAGTATATGAAAAAGAAGATTTTAAATCAAATATATATGACACTTATTTTAAGTTTCTAAACAATGATTTTATTATCAATAAAAATCAATGATTAAAAATTGCAAATTCTTTAGGTTTCGTTCCACAAAACTTTATATGCAAACAAAAGAAAAGAATAATTATTTTTGGTTTATTTTTACCTAATGAAATTTATATTAATAACCAAAAAATAGAATTACCTTATTATCACAATTTAGGTAACTTAACAATTAACTATATTTAATTTAAGGGAACTGCTGCCCAAAAGACGGAAGAAAGGAAGCTATGCATAAAATTATTAGTGTTTTATCTAGCAAACTAGGAATTGAAGAAAGTGAACTCCTTAAAAAACTTTCATTAAATGAAAATTCTTCTCAAAAGGAGATTGCTTCTTCGCTTGGAGTTTATGAGTTTTTCCAAGAAAAAGATGACTTGACTTCATTCATCTTAAATAAAACTCAAAACCAACATAAAGATAATCAAAATTTAAAAACTGAAAATGAAAAGTTAAAAAATGATTATCAAAGTCTTGAGCAACAATTCAACACCAATTTAGAAAATTATAAAAACTTGTCCGGAAAAGTTAAAGATACTTTTAAAAATGAATGAAACAAATACAAATTGAATGAAATTGATTTTGAGAAGATAGATGTAAATTCAATTAATCTAAATAATTTGTTTCAAAGTGTTGAGCAAGTGGCAAAAGAAAATAACATAACTATTAAGACTAAAGAACCTAATTCTGTAAATAATATTAACCAAGATTCTAGTTCTAATAGTTCTGATCCATGAAGTCCTTATATTAGAAATTATTAGAAAGGAAATTATATGTCTTATCAAATACCAACTTTAAAACAATATTTAACTGCTGAATCTGCTACTTTATTTGAACAAGGAGCTGATAAAGCTTATACCATATCAAATGGTGGTCTAACTAAAATTTCAACTTTATTGAATGCAATTTTAGAAACAAACTTTTTAGGTAGTACATTAACTCCTGCAAGTGATCCAAATTCACCAGTTATTGAATATTTAATTTCTTTAGATCAAGAATGAAAAACTAAAGAAGAATATACAAAAAACCCAAGAGCTCAAAAAGCAAGATATGCTAAAAAAGAAATCCGTTGAAATGAATCTCATGTTCAAGATATTCAATTAAATAAAGTTGATTTATTAATGAATGTTCCTGACATGCTTGTTCAAAAAGCAAGTGACGAAATTTCAAAATGAAAGAGAAAAGTTGACAAACAAAATTGACTAAAAGTTGAAGAACACATTAAAAACTATGCAACAAAAAAACAAAATGGACAACCTGATTCAATTGTCACTGAAATTGATTTGACTAAAAAAACAGATGAAGAAGTTTATAGTGAAATTGTAAACATGGCATTAAGATTAATGGAACATGAAGATTCAACATTAGGAATTGACGGAATTGAACAAAGCAAAATTGGAATTTTTGTCAAACCAAGTATTTATCAAAAAATAGTCAGATATTCAACACAAGGAAACTTTGTAAAAGAATCAATTCAAGGTAATCCTAAAGACCCAAGAACATGATTATTAAGTGATTTCCAAATGATTGCAGGAATTAAAGTCAAACCTTCAACATTATTAAAAGAATTTGAGGCTGTTGCTTGAACAAGTGATTCTGCTGGTTCTGGAAGCAGATTAATTTCTCTTAAAACTGACGAAGTTCCAAAATTTAGTGATAGATTTGTGGCTATGGAAATTCAAAACTTTTTTGGAATAGTTTGTGATTCAAACTTTGAAGCTATTAAAAAACCAACAACTGCACCAATTCCAAAACCTGGCTCTTAATAATGTTAATTTATTCTAACCCAAAACCAAAAACAACCTTGCAATTAAGCCCAAAATCATTTGTTATTAATGATTCAAAAAAACAAATGAATCCTTTTTTGCGAGGTTTTCTTTCATTCCTACATGTTGCTTTGGATATAGGAGCAAGTTTTTTAGGTAGTTTTATTAATACCTTTGCGCCTGGTCTTGGTCTTGTTATTGAATATGGTCTAAGTACTGCCTTAGATTTAGGTTTTAATGCTGCTATAAACTATGGAAATATCAATTGACAAGACTTTGGAATTAGCGCTGGAATTAATGCTATTCCTTTTGTGACAAGAGGAATAAGATATGCAGCAAACACACATAAAACTAACAAGTTTTTTCAAATTGCAAAAAATCTTAAAAAAGAGGGTCAAGAAGAATTAAGTGAAAAAATAACAAAAATAGCTGCTAATTATTCTGAATTAGGAGTTGACAAAGCTAAATTTATTGACAATAAATCACTTTTAAAAAAAGAACTAAAATTATTTAATAATGATTCATTTTTGAGAACTTTATCTGATAACAAAAATATTTATTATTTTTTGAATAAAAAGGTTCAAAATATACAAAAAGCTTTCACACATTTAAGATCTATGGTCTCATTATTAACCTCTCCCTCATATTTAGCAAAAAAAGCAGTTGACACTGTATTTAAACACCCAAAAGCCTATTTGAACAAACAACTTGGCAAATTTACCAAATTATTAAAAAATACTTTTAAATCAAAACCAATTAAGAAAGTAGTTAAGAAAGCTTCTAATTATATTTGAATTGATCATAGTTCTTGATTAGAAAGAATGTCAATAAATAAAGTGAACAACCCTTGAGATATTAAAGCTATAAATGCTGTTCTCTATTTTAAGAAAAATGCAACAAAAAGCAAAAAAAATCCTAAAGGAAAAGAACCTGTTAGATTATGAAATAAGAGCATAGATGAATTGATTAAATTAGTTTGTGCTAAAAGTCCTGGAAGATACTATTTAAATAATATTGCTTATGGCCATATAGTAGGAAGATTTATTAGAAAATTTGAAATGTTTCAAGCTGTTCCAATACACCCTATTTTCTCCAATTTAATAGGAACTTCATTATTTACTTTTAGAACAATTAATTCTCTTATTATGAATTTTAAAAGACAAAAATGAGTGTGACTAGAAGACAAAAACATAATCTTAAATGAAATTGTTAAAGGAGTTAAAGAAAATGTTTTTAAAGGTTGAAGAGCACCAGGATTTCAATTTATTAGCTCTATGGCTAGATCATGAGCAACTGGCAATGTAGGTTATCAAACAAAAGCCGCCTTAAGGTGAGCAAATAAAAAAGCTTTTACTAGTAAAACTCAAAAGACTAATTATTGAGTTAATCACTGAGTAAAGAATAAATATAAAAAATAGGAAAGGAGTTAAATTAATGGAAGAATTAAACAAATTAGAATTAAATTTGAATGAATTTAAATCAAAATATTGATATGAACAAAATTTAACTATTCCTTATGTTTTTAAAAAACAAAATGAAAATTTAAATGAAGTAGATTTTTTTAATAAATTTGCTTATTCAATATATACAACAATAGATTTAATCATAGGTCAAAAAATGAGTTATTTTGTTAAAACTCAAAGTGACTCCAAAGCTATTAAAAATATTATTGGAGAAACAAGATTTGAAAACTTCAAAAAAGCTATTTATTTGGAAATTAAATATAGACTTTCAAGTAAAAGTTTTCCAGAATATTCAAATAGTGTTTTTCTGGTTTCTAATAATATTTCACTTAATGGTGAAACAAGAGATTTTGGTTCATTATCAAAATTAATAAATTATGAATCTATTCAATTATTAACATTTCCAAGTTGAGTTGATTTAGAAAGTGACATTGATTTTATTCAAGCTGATTTTGAAGCTTTATTGAAATCATTAAAAGAATTTGTTAACAATAAAGATATTGAATTAGAAAACAAAATAAAAAGCTTGGAAGAATATGACAAATCTCAAACTCAAAATAATATTAATCAAGAAGCGCGTATTAAAAATTTAGAATATAAAACTAATGTTATTAAAAGCAAAAGCGAGATACGACTTGAAGACAATGAGGGAAAATTTCATTTATGAGGTAACGGTTTCGGATACAAAAATAATAACGATGTGCTTTATGCATTTCAAAATGAAAGTTTTAAAAAACTAAAAGACATTTTAGACAACTACGAAAATTTAAAGCAAGCTTTTGAATCCTTTAAAATTGCGCAAGAAAACCAAAATACTAAAAATGTCCAAATCAATTCCACACAAGATAATCAAATAAAAGCTTTAGAAGATAAAGTTGCTACTTTAGAAAATACCACATCTAATGTTTGAAAAATAGCTGAAATAGATAAAAATTTAATTTCTTTATATAATCAAATAAATAGAGAAAAAGACAAAAAATGAATAAGAGTCGGTTTAGTTGGAGAAAGAGAATTTGAATATGGCAAAGAGCACACAATTTCATTAGATAAAAAAAGGACTAATCTAAATTATGAATTTGACCTAAAAAAATTTAAAATAGTAACTTATATTAATTATGCAGGGAAAGAGAATAACTTTATTACATATTTAAATTCTGGTGATGAATTAACTTTTTGTCAGCCTGTGGTTTATGATGATATCGGCTCAAGTAGTGCATTAGCAATATGAAATATTAAAATATTAAATGAGTCTTGTGAATTTAAAATTATACAAGTTGGCAAAATTTTTAATAATGAAACACCTAGTGATTTGCGTTTTTGAAAAATAGACTTATTTAGCTGAGAAAGAACATATTAATGATTAAAAGATTTTTCTTATGACTATTAAGCATTTTGCTTATATTATTAATTATTTCAATATGTTTTTTAGTCTTCACCATTGTTAAACCTGAAAGCGCAATAAGTCAAGAATTAGCTTCATGATTAAAACACCTATTTAGAATAAAAATTGGAGGTTAGAATAAAACTCTATATTTTAAAAATGAAAATTGAAAAAAGAAATTTAGCTAAAAGAATCAAAGAAGCTTCCAAAGCTTTGAAAACAAATACATTAGACAATAAAATTGAACATGATAAGAAAGGAAACAAATAATGAAAGATTTTTTCGCAAACCCAACTAACTTAATTATATTTTTAGGTGGTTTTGTTTCTTTAATAGCTTCTATTACTGGAATCACAACCACTTTCATTAAAACTCATAATTTAAAGCTTCAAAATAATAACCTTAAACAAGAAAATAAAGACATAAGATTTGAACTTCTTCAAACTCAAAAAGAATTGGCTGATAAAAACAATTCACTTTCAAAACTTGAAGTTATGTTTGACAACCAAAGAAAAGAAATTGAAAGACTAAGTGAAGAATTAAAAGCTTTACAAAGTGGCAATGTTGATCCTTTGGTTAAAAAGCTAAAAGAACAAATTGAGAACCTTGTTAATGAAAAGAAACAATTAACTGAACAAGTCTATAATGTTAAAGCTGAATTAAATGAGTCTAAATATCATTCTGGAGCTTATGCAAATGAAATTGTTGAATTGAAAAGGGAAAATAGTGAGCTTAAAGAGCAATTAAAGCTTGAAAGAGCTAAGATTGTTGAAGACATAGCAAATATATTAACTCCCAAAAACAATGAGGCAAATAAGGCTTCTAATGAAGCAATAAAGGAAAATAAAATAGAAGCCCCAAAAGCAGAAACCTTTGAAGCTCCAAAAAATAATGAAAATGTTAGCCTATATATTTAAAAAACCTTTATAATATAATTGTGAGTAACATAGCAAATGGCAGTCGCCATTTGTTTTTATTACTTAATATTTTTAATTAAATTTTTTAATTTACTTATATTGCTATTTAATACTTCTGTAAAATTAACATTTTCTTTTGTTTCACCTAAAACTTTTTTGTATTTGTCTAATTGGTTGTTTAGTTCACTAATTGTTAATTTGAGTTGAGCATTTTCTTCGCTTAGTTCTTTATATTTAATTTTAATTTTTGAATAGTCAAAAGCACTTTCATTTTCCATTTGAGCTTGTTCCATAATATTTTCAAGAGCAACATTGCTAAAGTTTAAATATGTTGTTGCTGTTGTTTGTGAGTTAACATGACCAACAAGCTTTGCAACTTCTTCTGTTCTTCCAATGTTTAAATGAGCATTAACAATAAAGAAGTGTCTTAACATGTGAGCAGAGATTTTTATGCCTCATTCTGGCTCTAATTCATGAACATAATGTTTAAATAAATTGAATTTATTTTGAATGGTTTTAATGTTTAAACCTTTTTTAAAGAAATCAATATTGTCAATAATGTCTTGTCTTAAGTTTGCAAAATAATGATTTGTTTCAGGTTTTGGAATTGTGAAAAGTCTAGTGTTATTTTTCTTTTCTGTTTCAATTCTTTGAGTGAAATTATTTTTAACTAATAAAGTTCAATTAACATTTGCAAGTTCTCCAATTCTAACTCCTGTAATAGCTAAAATTTGGAATACTAATTTGAATTTAAGATCTCCCCATAATTCAACATAATTGAATATTTTTAAATATTCTTCCATACTATATGCTTGTCTAGTTCCACCAACTTTTTTAAATGAATCTAAGTCTAGTTTAGTATTGATATAAACTCCTGTATATTCTGATAATCATTTGATAAAAGTAGCATAAACTAATCTTGTACTATATTTAGTGTTTTCTGCTCAATTTGAAGAAGCTAAATAATGATTCATTTGGTCTAGTAATTCTTCAACCGATCCAAATCTTTTAAGTCTTTTCAATAAAGTTAAATAATGTTGAAAAGTTTTTTTACTTTTTTGTTTTCTTTCAACTAAGATTTTAAATTGATCTAAGTAAACTTTAACATAGTCAATTTCTTTATTTAAAACAATTTTATTTTTGTAAAACATGATTACTCCTTTTTCTCTAAAACAACTATAAAACAGTTATTTTAGTTAATTTTATTTATGTATTTTTATTCAATTAATTAATCTTTGACAACATTTTTGACAAAAATAAGGGAACACATATTCTTCATTTTTGTAATTAAAAACTTCTCTTTTTTTGTCATTTGTTAATGTTTGACAACAACGGCAACGCCATCTGTTCATTTTTATTTTTTCAAACATAATAGTTCCTTTTCAAATTTAATCATTTGCCTTTTCCCTTTCTTTCAATTTGCCATCAATTGCGGTATATACCATATTTAAATAATCTTCAAATACTTGCACTTGTACTGGAGTTCTTAAATTGTCAAATTTTCATTCGTTAGCTTTGTAAGACTTAACACATAGCTTAATGTTTTTTAATCTTATTTTCATTTCTCTTACTATGAAATATAATAGGAAATTTGATTTAAATAATTCCATCATATTGCTAATTCAAGTAAGATAGTATTTTATTTTTCTTCTTATTTCTTTATATCAAAACTCTATAAATTGTATATGAGTCATTAAATGACTGCAGCCATGTTTAGAAGAATTGACACAACTCATTAATTTGTTAATATCATAACTTAATGGTAAGTCTGACATATCACTAACCAAAGCTCACAAATAATTTAAATCTTTGTCACTACAATTTTTAAATGTTTCTCTTGAAATTAACATTTTTATTTTCCTTTCTTTATTTTATAAATCTGGATACTTTCTAAATTTTTCATAGATTTTATCTTGTGTTTCCTTTAAACTTTTTAATTCTGCTTCTAATTCTTGTATTTTCTTTTCTTTTTGAGCTTTTCTAGCTCCTCTTAAATTAGAAAGAGAATTTATATTTTTTCATAATCTTTCTTCAACAATATCTATTCTTTGTTCTGTTTGTTTTAGAAATTTCATACCATTTCTTTCAAAATATTCTTTTTCACTTATTAACATTTTTATTTTCCTTTCTTTCTTTTTTAATATATAATAAATTTGCGGTTGTTTGGTGTTCAATTAAAGGCAAATAACCGCCTTTTTATTTTGTTCAAACCGAACAGACAATGATAAGTAAAAACAAGAGTGAGTTCAAAACCCACTCACTCTTTATTATTAATTATGAAAATAATAAATTTAGTGATTAGTAAAAAGTTTGCAGGTTTTTACTTATCACACTTATTATATCGGGAATATATAACAATGTCAAATAAATTTATGAAAATTTTTCACGTTATAATTTAACTATGAAAATGCCAAGCTATAAAGATCTTAATTTTAATATCAAGAGAGAATTAAATAAATTAAAACCTAAAAGCTTTGAAGAATGAAAAGAAGCTAAATCAAAAGTTTTTTGCCTTGCCCTTGGTAAATATTTAACCAAAATTAATAGCCACCAACCTGATATATTTAAAAATTATTATCAAGTTCTTTTTTATCATTCATTTATTAATTATAAAAGAGAAGAAGAATTATTGTCTATTCTAAAAGCTAAAGGTTACCAAGCTAAGAGAACAAATCTTAAAATGGATGGCATATATAAAATTGATATAGTGGCAACAAAAGGAAATGAGAAGATTTATATTCAAGTTAAGTTAAACAAACCAAAAGCTTTAGAATTGAAGAATTTGCATTCATTCTTAAGATCTAAGGCCTTTCAAGGACTAATTGCATGAAAAGAGTCAACTGAGTGAAAATTCAAAGAAATTTAACATTTTTTAACATATTTAAAATTTTGATATAAAATAAAGTTGAAAAGTGAATTGTTTTGTTCACTAAAAATCTTAAATACCAGATATTGACGGGAATATATAGTAGGAATATATAAGGGAATATATTTTTATATAATGTCAATTGTGTTAACAATTTGTTATATTTAACTATAATTTTTAATTGTTCTTCAATAGAGGGAACTGGAATAAAAAACAATTTTATGAGCTCTATTGAAATATTATCATTTGTAGAATTTTTATTTTTTCTGATAATTTTTGATATTAGTTCACTTTTCATAACAATATCTACATAATCAGGTAACATTTCATTTAATATTTTTATATTGCCAACTCTTTGATTTACAAGAATTTTTTCTTTTAATTCTTTAATTAATAAAGATTTACCAACTGTTCCACCTGTCATACAAATAACAATGTTTTTATTATTTAAAAAATAATCAAACATTTTACTTTCATATTTATAATAAACAATATTGTTATTTTTTAAACCACGTTCATCAAAATCAGATATTCTTAATATTCTTATACCATTTTCCTTTGACACAAATTCTGAACTTTTAAAAGCAAATCCTCCATTGATAATTGAAATATTTTTTAATCTTACTCACATTCAATTCTCTGGTATTTCAAAAGGCAATTCTTCAGTAATATCTTTAATATTTTTGCCTATCTTCTCATAATAATGATTATCCTCACCTTTAAAAATGAAAGATTCTTGTTTATCTTTCTTTATTTTTCCTTCTTTAATTAATTTTTGTTTTTCTTGATAAATTCTCTCAATAAGTTTGCTAGCAGGCTCATCATTTGGATCTTGTTTAACTAATTTTCCTTGAATAGCCAATTGAAGAATAGATTTTCTCAATTGTTCTGCTGTAAGTCTATTAATCTTCATTTAAAATCCTTGTTATTTCTGCTAGTATTTTATCTATTTCATCATTTAAAGTTTTTCTTTTTTCTCGATATTGAGAGATTAGTTCCATTGGTTCTAATATTTCTTCTTCTTCATGAGGAAATTGGCATAAATCTAAATCATAATTTCTATTAATAATTTCTTGTTTTGAATATTTTTTAGCCTTTGGGAATTCATCAATTATTATTTCTTCTCTATTATCTCATCATTTAATGACTTGATCAAAATGTTCTAATTGTATTGGTTTCGTTTTAGAAAAGTTTTTATATCCTTCTGGCATATCCATTCTATAGAATCAAACTTCTTCAGTTGGATGAGTGTGATCAAAAAACAAAATATTTGTTGTTATTGAAGTATATGGAGCAAAAACACTATGCGGCATTCTAATAATGGTGTGTAAATTAAATTCTTTTAATAATTTAGTTTTTATTGCAACTTTTGCATTATCTGCTCCAAATAAAAAACCATCAGGCAATATAACTGCACAACGTCCTTTTTTCTTTAAACGATACATAATAACGTTCATAAATAAATCTGCAGTTTCACTACTTCTTAAATCAGCTGGAAAATTATTTTTTACAGCTTCTTTTTCTGAACCACCATATGGGGGATTCATTAAGATAATGTCGAATTTATCAGCTTCAGTATAATCTTGTACTCTTTTTTCTAAAGAATTATCATGAAATATTTTTGGTTCATCTACATCATGAATTAACATATTAGTAATACATAAAAGATATGGCAATGGTTTTTTCTCAATGCCATAGACTGAATTATCATATAAGTTTTCATCTTCAACTGTTTTCTTTTGATCCTCTAAATGCTTCAATGAAGATGTTAAGAATCCACCTGTTCCGCAAGCAAAATCTGCAATTTTTTCTCCTAATTTTGGATTAATCATTTTAACCATAAAATCAGTTACTGCTCTTGGTGTATAAAATTCACCTGCATTTCCAGCACTTTGCAAGCTTTTTAAAATAGTTTCATATATAGTACCAAACTCATGTTTTTCTTTATAGTCAGCAAAATCTAATTCATTAATTACATTAATAACTTGTCTTAATAAAACACCGTCCTTCATATAATTTTGTGCATCTTCAAAGACAAATCTTGCAATTTTTTGTTTTAAAATAGTATGTTCATTAATTCCATAAAAAGCTTGTTCGCCATTGCTCAGTGTAACAGTTTCTCCTTTTAAAAAAGGAAAAAGTTTATTATTAACTAAATCTAATAGCTTTTCGCCTGTAATTACATTGTTGCTATGATCATCAGTTGCTCATGTGCTTCATTTTAGTTCATTTGGTATTAAGCTTTTATAATCTTTATCTATGAATTCTCATTCTCTTTCTTTTGCATCATATACTTTAAGAAAAAACATTCAAGTCATTTGACTAATACGTTGAGCATCGCCATCAACACCTGCATCGTTTCTCATAATATCTTGAATTCTTTTAATTAAATTTCCAATAGCCATTTTGCCAATTCTCCTTTTTTATACTATATAAAGTGCTTTTTGAATTTCTTTAACAGTCTGAATATATTGTTCTTTTCCACCAAAAAGTTTAGCAATTTTTAAAGGACCGCCAAATTGTTCAAATTCTTTTAATCTCAAATTATTGTTATCTGTTAATTCATCAATGACTTCTTCTTGATATTTATTTATAAGAATTTGTAAAATTTTTCTACATGTTTCAACATTTTGTCTTTCTATTATTTGAACAACTTTTTTATTATTTACTCTTTCAGATCTTTTTAGTAATTTTGATCCATATGCAATGCTACAGATTAAATCGAAATCATCAAAATCTCTTGGATAAATATTTCTTAAGTTTTCAATTAAAATGCCTTTGCTCATTAATTCTTGAATTATGGTAGATCTTGTTTTATTACTAGTTCAATATTTAACAAAATCTTGAATAGTGTTATAGTTTTTTAAAACATTTCTTTTTACAAAATCTTCAATAGGTTCAATAATTAATTTTCCTTCATCATCATAATAGTTTACTTTTTTGTAGATTAATTCAACATCTACACCATTAACATATATTTTCTTTCTTCTATTGCTTTTATTTTGTTCTGAGTTTTCTTTTCAGACTAATCCCTCATCAACTAATTCATATTCTTTTTTTGTTTCTTGTTTTGCTTTTTTATTTCTAAGAATTTCAGGATTAAAAAATTCTTCTTGGATTGCTGGGCCATTAAAATCAGGATCAGCAAATAATTTAGTAGCATTTTTAAAGTCTAAAATAGTGAAATACAATTTGTTTTTATGTTTATCTTTAGGATCATCAAATTCTTTAATTCTGGTGCCTCGGCCAATTATTTGTTTAAATTTAGTCATACCATTTTCACCAAAAGTATTATCAAGTGCAATAACACGACACAATTTACAATTGACACCTGTAGTCATAAGTTCACTGGTTGTAACTATTGTTGGATATAGCTCATTATCATCAATGAAATTATCTAATTGTTTTTTACCTTCTTCATTGTCACCAGTAATACGAACAATATATCTATGATCTTTGGCCATCATATCACTATTGTAGTGAACTAAAGCTTGTCTCATTCTCTCAGCATGTTCTATGTCGTCACAAAATACAATTGTTTTTGCATATCTATTGTTAATTGATTTTAAATAATCGGTAATTTTTTTAGCTACCTCCTTTGTTCTTTCTTCTATTACAATAGTTTTATCAAAATCCTTTACATTGTATTCATTGTCTTCCACTATTTCACCATATTTATCTCTTTTATCTTTTTCTGGTCTATAGCCAAAAACATCTGTATTTATACCAAATTTAACAACTTTGTAAGGAGCTAAATAACCATCTTCAATACCTTCTTTAAGTGAATATGTATAAATTGGTTCACCAAAATAATCTATATTTGATACTTCAGCAGTCTCTTTAGGAGTTGCAGTTAATCCAATTTTAGTAGCAGAATCAAAATAATTTAATATTTCTCTTCATTTAGAATCTTCACTTGCACTACCTCTGTGACATTCATCAACAATTATTAAATCGAAAAAGTCTGGTTTAAAGCTTTCTTTTAATTTTTTGAGTGAATCTTCGGAGCCATTTTCAGATAATTGTTGATATAGAGATAAATAAATTTCATAAGATGTGTCCATATTTCTTTTTGAAACTTTAACCATACTATTTTTGAAAGGTTTAAAATCCCCTATTATAGTTTGATCAATCAAAATATTTCTATCAGCTAAATATAAAATTTTCTTTTTTAAACCAGATTTTCACAATCTCCAGATTATTTGAAAAGCAACATATGTTTTACCTGTGCCAGTGGCCATAACTAGTAAAATTCTATCTTTTCCTTTAGCAACAGCGTCTACTGTATCATTTATGGCTCTTTTTTGATAATATCTAGGAATATGAGAATTTTGTCCATAATAATAAGGAGTGGAAATTATTTTTTCTAAAGCCTTATTTTCCTTGATTTCTTCACCATATGTCATCTCAAATAATTCATCGGGCGTGAAAAATTCATCTAACCCTATTGTTTTCTCTATTCCTGTTTTCATATCATAAAATCGAAAATTGTTACCATTTGATGTAAAAACAAAACGAACGCCCAACCTTTTAGCATAATTTTGAGCTTGGAGTAATCCGTCGCCTTCTTTATGATCTTCACCTTTTGCTTCAATAATTGCGAGCGGTGAACCATCTCCATTGTAATTCAACAAATAATCAATTCTTTTTGCTTTTAGTTTTTTAGCCTCACCATTTTCGAAAATCACTTTTCCAGCAGAAATATAATATTCCATATTTATGAAATTTTTATCTCAACCAGCTCTTTCTAAAGCTGGTGTTATAAAACGTAATTTTACTTCTTCTTCATTTATAGTATTTTTTTTATTCATCTTAATAACCTCATTTAAATAAATTCCTATAATATATAATTATAAGAAGTATTATTATTAATATAATTTTATCATTTAAAGGACAAAATTAGAATGATAATTTTATTAAAACATTTAAAATATAAAGAGAATATACAAAATTATCAACACAAAAAAACTTTTTATTTTGTAATAAAATAACTTGTTTTAAATTATTTTTTTGAAATTTTCTATTTTATAGTAACAAAAACATCAAATGGTTAGCCACACTAACCAGTTCATTTTTTTCTTATTCAATTTAAAAAAAGATAAAATAAATATATGAAAAATTCAATAGATCAAAAAAGTAAAATAAAGGCTTATAACTTATTTGACTTAATAATAGAAGATAAATTAGAAATAGGCACCACTAAAAGTTTAATGCAAATTCATGGTTATTTATTTGGTGGTCTTTATTCTTTTGCTGGTCAAATTAGAAAAAAAGATATTTCAAAAGGTGGCTTCAGATTTGCTAATTCTACATATTTAAATGAAACACTTAAAAATATTGAAAATATGCCCGAAAGTAATTTGGAGCAAATAATAGATAAATATATTGAAATGAATATAGCGCATCCATTTCTAGAAGGCAATGGACGAAGTACTAGAATTTGATTAGACTTAATGCTAAAAAAGAATTTACAAAAAGTTGTTGATTGAAGTTTAATAAATAAATTTGATTATTTACAAGCTATGCAAGAGAGCCCTTATGATGATACAAAAATTAAAAATTTAATTAAAAAATCATTAACCGATAAGATTAATGATCGCGAATTATTTATGAAGGGGATTGATTATTCTTACTACTATGAAGAGTAATCTTTTTTTATGTTTCAATTTATTTAAAAGACCTGTTTTATAGGTTTTTTAGTAAAAAATCAAATATTAAATAGTATCTAAATACATTTGATTATTTATGTCTTCTAAAGGTTCTCTAATTTCCTTTTTAGGTACTAGTCAATGGCTTGAAAATAAGAAATTTAAAATAGTTAAATTTATAATTCCGGTAACTATTATTCAGTCCATTCTAATTTCAACATTTGAATAATCATCAAATTTGAAATTATTGTCAACTAAATAATTAATTTGGTCATATTTGTATCATAAATCTCGATAAAAAGGATTGCCATAAACAAAGTAAATTAATAATGACTCATCAGTAAATTCACTAAGATAAAACAACATATCATGTCAAGGTTTACCACCTAAAGAACTTTTAGATATATCTTCATTTCTAACTAAATCACATAAATTAGCAAGAATCATATTATTTATATCCATTAAATCTTTATATAATCTGGCATATTTAATATTTGCTAAATTACCTTTTAGTTTATATCTATAGTCACTTTTGTCACCTACTTCTTTATTTTTAATTGCTTCTTTAACTTTGTCGAATAATTCCTTAGGTTTAATTTCTTTCATACAATTAATTGTTTGAGTGTAATACTTTTTAAAGTTTTTGATGAAGAATTCAGTATTAAATTCATTACATTTAGCTTCTTTATTACTATCAAATTTATTAAGAACTGCTCATTTTATATGACTATATTCAATTTTGTCTAATGTGTTTTCTTCTAATTTCAAGTAAGAATTTAAAAGCTTTTTTATTTCTGAATTAGCAATTTGTCCACTATTAAATTTAGTTTCACTTTTTTTGTGTTGATCATAATATCAAGAACCAAATTCTTTTAAATCTTCTTCATAAAAAGTGTAATCTAAAAATAATTGTTCTGTTCATTCTGGAATTAAATGAATTTCAACATCCTTGTCACTAATTACATTAACACGTGGAATATCTATTTGAAAGTTTTTTCTTCCTAAATCAGCTTTTAAAGTTTTTTCATTATTTAGAAATTCAATAAAGTCTTCTCTGATAGTTAAACTATTTGCTCAATTAAGATATCAAATACTTTTACCTTTTGAATCACAAAGTTGATAAGCCACTACTTTAGATGTTTTTTTGTTGCCTATTTCGCGTTTTATATAATTTCTAACTTCTTCATACATTTCTAATTCATATTCTTCCATGATGTCGCAACCAACTTCATCATTAATGTCATCTGAAGTTATAATATTAAATAGTTTTTTCATAATTTACCTCTCTTTTTATATAAATTTTAAATAAATTGCAAAATATTTTTTAGAAAAATACATAAATAATCAAAATTTAAAAAACAAAAATTTAGATTTTGGATTATTTTAATTTGATAAAATAAAATTATGAAAAGAATAAAGATATTTGACAAAAAATTAGTAGAAAATTATAAAGATACATATTCTTATTTAATTAAATGAATTGAAGAAAATCCGCATGAATTTTTAGCTTGCTCTCAACATCAAATTACAGAAAAGATTTATGTATCTCAATCTTCATTATCTAGATTTACAAAATTTTTAGGTTTTGAATCATATCGTGAATTGCAAATTTATGTTGCACGTAAAGTGGAGCAATTTTTACCAAACGAAACCTATAAAATTGATGAAATAAAAGATGCAAAAGATTGTATTTTAAAAGTTTTTCAACAATATCAAAATATTTCAAAAGCAGGTTTTAATACAATAGAAACTATTCAAATTGAAAAACTTGTTAATAATTTAATCAAATACAAAAAGTTAATTATTTTTGGAATAGGTACTAGTGGAATTATAGGTTCTTATTTAGCCAAGCAATTAACAAGAATTGGAATAATTTCTGTATGTATAAATTCGATCCATGATTTTAAGGATGCATACAATGAATCAAATAAAGATGATGTTTTTTATATCATTATTTCTAAATCATTTAAAAATCATGAAGTTAAAGAAGCTGCAGAAATACTAGATAAAAGCAATTTTAATTTTCAAATATTGACAAGAAACGAAAATGTTTCTTATGCAAATTGCACCTCACCAATAATTTATGATTATATTCCTGCACAAAAAAACTTTAATTATGATATTGTGTCAAAATTGTCTTTATTTATGCTCATAGATATAATTTATATCTACGCCAAAACCTTAATAGACAATAAAAATAAGTAATTAGTGAATAAAAAATTCAAAAATAGTGAAAAAAATTCGGTTTTTTTCATATTTTTTTGCATTTTTTGACTTTTTATATTTCATTACTGAAATTAAATTCCTAGAAAAAAGAGGTCAATATATATATTGATAGAATAAAGTTGAGGAGGGCAAGTGAAAGAAAAATTAGTTAAATACTTAAAAATGACTATTGTTATTTTCTTAGCTGGTAGCCTAATTGGAATTTATTTTCTAAAAACAGGTCAATTTGAAAATCATAGTCAAAAAATACTTTTAGATAGATTAAGTAATAATTACAACCGTAATTTTGCTTGACTTTCATTAGCTATTTTTGCAATCGGATGAGTTTTGTGAGAATTCGCTATAGCTAAAAGTGGTAATAAAAATAAAGCTTATGCAGCTATTGCTTTTATAGTTGTTGGAAGCACTTTAAGTTTAAATATCATTAACTATATTTGCACTTTTATTGCATTAATCATAATTGTGCTTTTAACAGTTACGTATGTTCCAAAAGTTCAAAAGAAATTGGTTATTGCTGATTTAGAAGACAACAAGAAAAAAATACAAGAAGATAACCAAAAACTTAAAGAGGCTATTAGCTTTAAGAAAAAAGAAGAAGTTGTTTCTGAACAAGAAACTTATGAAGACGGAATTTAAGGAGATATTATGAGATTTAAAACAACATATGCAGTTTCAGCAAATGAAACATCAAGAATGACAACAGAAGAACTGAGAAGTAATTTCTTAATTGAAGATTTATTTGAAAGCGGAAAGCTTAATGTTCAATATCTTCACTATGACAGAATAATTGTTGGTGGTGCAACGCCTACATCAAAAGGTTTAAATTTAGATGAAGTAGCAAAAGAAATTGCTGCACCATATTTCTGTGAAAGAAGAGAAATAGGAATTATAAACATTGGCAATACTGGTTATATTGAATTTGACAATGGAATTGAAAAACTAGAAAATAAAGATGGTTTATATTTACCTGTAGGTAACAAAAAAATTATTTTTAAATCAGCTGATAGTAAAAAACCTGCAAAATTTTATTTCTTTTCAACTCAAGGCCTTAAACCACTACCAGTTAGAAAAGTAAATATTAAAGATGCAATTCCAAATAAATTAGGAACACCAGCTGAAAGTAATGTTAGAACAATTTACCAATATTTTCATCCAAAAGTATGTCAAACAAATTCACTATTAATGGGGCTTACAATTTTAGAACCTAACAATATGTGAAACACAATGCCATGTCATACACATGAAAGAAGAACAGAATGCTACTTATATATAGATGTAGCAGAAGGTCAAAGAGTAATTCACTTAATGGGCAAACCTCAAGAAACTAAACACTTAGTTGTTAAAAATGAAGGATTTGTCATTTCGGCTCCTTGAAGTATTCACTCAGGAGTAGGAACAAGTAACTATGCTTTTATTTGAGCAATGGGTGGAGAAAATCTTGACTATACAGATATGCAAGGTGTTAAAACAATAGATCTAAAATAGTGTTTAGAAATCTAAATAATTCATTAATCTATGAAAAAGAATTCAATGATACAAAACTATTAACTAAAGAAGAAATTAAAAAAGCTCTTGATTTAATAATCAAGCAAGTTGATCAAAATATTGATTATTTTGGCAAAGAATTTATGATTGCCAACACATTCAATAATAAATATCAAAAAATTTATTCTAGTGAATGAACAGATGGATTTTGAACAGGAATTCTTTGATTAGCTTATGAATACACTAATAATAATAAATATAAAGAATTAGCACTTGAGCATGTCAAATGATACAAAAAAAGAATTAAAGAAAAAATATTGGTTGATCATCATGACATGGGATTCTTGTATTCACCAAGTTGTGTTGCAGCATATAAATTAACTAAAAATCAAGAAGCAAAAGAGGCTGCTCTTTTAGCTGCAGATAATCTTATAAGTAGATATCAAGAAAAAGGCAAATTTATACAAGCTTGAGGAAATATTGGGGCAAATGATAATTATCGTTTAATTATTGACTGCTTATTAAATATTCCTTTATTATATTGAGCTTCTGATTATGATAGAAAAAATTATTTCGATAAAGCATTAAATCATTTTAATACTTCTTTGCATACTGTTATAAGAGAAGACGGAACAACATATCATACATACTTTTTTGATGCTAAAACAAACAAACCTTTATGTGGTAAAACTAGACAAGGTTATAGTGATGACTCTTGCTGAGCAAGAGGACAAGCGTGAGGTATATATGGAATTCCACTTACATTAAAATATGCAAAAAATAATTCAAAAATAGATAAAAACGCTTATCAAATTTATCAAAAAGTGTTAAATCAATTTTTAAATAATTTGCCTCAAGATGATTATGTTTGTTACTGAGATTTAATTTTTAATGAAAAGGCAGATCAATCACGCGACTCATCAGCTAGTGCTATAGCAGTTTGTGGTATTTTAGAAATGCTTAAAATTACTCAAAAAGACTATAAAACAGATTTATACGAAAAATCAGCTCATTTAATTTTAAGAAATTTAATTAATAAATATCACAATAAAAAAATTGAACCTGGAACACCAATTTTATTGCATGGAGTTTATTCATGACATTCAAAAAAAGGTGTTGATGAAGGAAATATTTGAGGTGATTATTATTACTTTGAAGCACTAATGAGACTATATAAAAAGGATTGAAATCCTTATTGATAAAAGGGAGATTTTATGGCAAAAATTGTTCAATGTAGAGTTGATGAAAGATTGATGCATGGACAAGCATCTATTTGAATGCAAGTAAATGGATCAAACTCAGTTATTGTTGCTAACGATGAAGCAGCAGTTTCGGAAACTCAACAACAATTAATGAAAGTTACTGTACCAGAAGGGGTTAGATGTAGCTTTTATTCAGTTGATAAATTAATAGAAATTTGACCTAAAGCTGCTGATTGACAATTAATTTACATTGTTGTTAAAAGTATCGATGATGTTTATAAATTAGCTTTAGGAAAATTACCAATTGAAGAAATAAATATTGGTAATGTTCATACAAGAGAAGATCGTGTAAGAATTACACCTTCAGTTAATCTAAGTAAAGATGAAAAAGCAAAAATTAAAGAAATGATTGATTTAGGAATTAAGTTTAATACTGCAAGCCTTCCTGGTGTAACTAAAGGTTTAGTTGACCAAAATAAATTAATTAGTTAGGTAGGACAAAATGGAAATTTCAATTATTCAAGCTATCTTTATCGGAGTTTGAGCCGCGGTAGCAATAACAGGTACTATGATGGGGACCTATACAGCAACACCAATAGTTTATGCTTTAGGTGTTGGAATAATTTTAGGGGATATTCAAACCGCTGTTATTGTTGGAGCTGCTGGTCAAACTGTTTGATTAGGCTTTGGTATTTCACAAGGTGGTGTTAGACCGCCAGATCCAATTGCACCAGGTATTTTTGCCACAGTTGTTGCAATTGTTGAAAAACACGGAAGCGCACAACCTATTTCAATTGGGGATGCTGGTATTTATATTGGATATTCAATTCCAGTGGGAATCTTAATGCAATTATTAACAACATTATTATTCACATTAATGTCACCGATAAGTGAAATAGCCAAAAAACAAATTACAAAAGGCAAATTTAAAACTTTTAAATTCTTATCTCATGTAACTATTTTAGTCTTGATGATCATTACATTTGGATTTGGTTTATTAGTTGGTGTTTCAGCTAATGGAATAGCTAGATTCTCAAGCAGTATTCCAGATTGATTAAGAACCGGATTAAGAGTTGCTGGTGGTATGCTTCCTGCTTTAGGTTTTGCTTTAATATTAAAAGTTATGCTCAAAAAAGAATATATTGGTTTTGCTTTATTAGGTTACTTTTTAGCTTTGGTATTTGAAGTTATTTCAAAAATCACAGGTCAAGCATTCTCGGTATTAGCTCTTGCTCTTACTGCCATGGCTTTTGTCTTGATCATAATATCAATAAAAAAATTAGCAGGTATTAAAGAAGCAGCTGGAGTAGCTCGAAACATAAGTAACACAAGCAGTAATAATGAAAATAACAAGGGAGGTCAACAAGATGGAATCTAATAACAAAGATTTTGTGCAAGTTAATCAAGCTCAAACTAAAAAGCTTTCAATTTGAACTTACATTATTGTTGCTTTAAGAAGTTATTTTCTTCAATCAGGCTTTAACTATGCTAACTTTCAAGGTTTAGGCTATGCCAACATAATTTATCCTGCTTTAAAGAAAATTTATGGTTATGGAACTCAACAATTCAAAGATGCTTTACTAAATAATATAGAATTCTATAATACTAACCCTCAATGTATTCCGCTTATAACTTCAATTCACATTCAACTTTTAAACAATGGGACAAGTTGAGCAGATGCAAGAACTATAAAAATGTCATTGATGGGACCTTTGAGTGGTATTGGGGATTCATTAACACAATATGCAATTTATCCTTTATTTTCTGTTATAGCAATTGGTTTTGCTCAAAGCGGAAATATACTAGGACCTATTATCTTTATAGTAGGAATGAACATCACATTAATAACAATCAGATTAACTTTAGGTATTTTAGGATACAAACTAGGTGAAGTTGCAATAGCTAAATTATCTAAACACATGCAAAGCATAATTAAAATTGCTAGCATGCTTGGGGTAACAATTATTTCAGCTCTTGCATTAAGATTAACAAAAGTTAAATTTACTTTACAATTTTCACAACGTGTTGATGCAGGTGCATCAGGATTTCAAGAACAAGTTATTTCAATTCAAAATATTTTAGACAACATTATGCCACTATTTGCTTCAGGAATATGAGTTATCTTTATTTACTTTATGGTAACTAAATATAAATGAAGTCCATATAAAGTTATTGTAGTAACTATTTTAGTTGGTGTTACTGCTGGAGTATTTGGAATTTTAGGTGTTTAATGGAAACAAAAATTTTGGTAATAGGACATTCACAATTTCCCCGAGTTGTTGTTGAAGGTCTTAAATTTGTTAGTGGTATTGAAGAAGACATTGATTATAAATATATAGATAATGATCATTCAATAGATGAACTTAAAAATGATTTAGAAAAATATTTAGAAAAATGTGAAAATATAATCATTTTTGCGGATTTACCAGGCGGATCACCGCATCGTCTAGCGGTTGAAACTATTGAAAAAACTAAAAAGAAAAATGCAATAGTTATTGCTGGTGCAGGAATGCCTATGATTATGGATATTGCTTTTAAAGTTATACTAATAAAATTCAAATCATTTGACGAAATAATCTGTTTTATAGGTGAAAAGAAAAAAAATTTAGATAAATACATTATGATCACAGGAGCTTAATTATGGATTTAGCAGTTAAAATTGTTTTATTTATTTTACTAGGTTTATTTATTCTACAAATAATAGTTGTAACAGTTATTTTGCCTATTTTAAAAAAGAAAAGAGATGAAAAAATAGCAAGGGAACATAAAGAACTAGTTAAGAATATTAAAAGTGGTCATGAAGTTTTACTTCAAAATGGAATTTATGGAACATATGTAAATTCTAGTGGCGACGAAACTTTTAATATGAGAATAAATGAAGAAAATAATGTAATTATCAAAGTTAACAAAAGTGCAATACTTGGTTTCTTTGACAAGGATATTGCAAAAAAGATTGAACAAAGAAAACAAAAATAATGGGAGACTATCATGGATTTTAAATTTAATAAAGACTATTTTTCAGTTAAGGACAAAGTTGTTATTATTACAGGTGCAAACATGGGATTAGGTTTAGCTTATGCTAAAGCTTTATCAAATTGTGGCGCCAAATTAGTTATTAGTCATTTTGACACAAATACAAAAGAAATAGAAAACTTTTGTGCTAAAAACAAAACAGAATTATTGCTTGTTCAAGGTGACTTAACTAAAGAAGCAGATAGAAATAAATTAATAGAAGAAGCTATTAAAAAATTCAAACATATTGATGCTCTAGTAAACAATGCTGGCACTATAATTCGTGAACCAATATTGGAATTTAGTGAAAAATCTTGAAATAAAGTTATTGATATAAATATGAATGCTGTTTGATTCTTAAGTCAAAAAGTAGCTCAAATAATGAAAAAGCAAGGCTATGGAAAAATTATAAATATTGCATCAATGCTTTCATACCAAGGTGGAATATTAGTTCCAGCTTATACAGCTTCTAAACATGCAGTTGCAGGATTAACAAAAGCTTTTACAACTGAGCTTGCAAAATATAACATTCAAGTTAATGCAATTGCTCCAGGTTATGTAGAAACAGCAAATACACAAGCAATAAGAGATGACGCTAAAAGAAATCAATCAATTTTAGAAAGAATTCCTGCAGGTCATTGAGCAAAACCAGAAGAAATTGCCGGTGCTGTTGTATTCTTGTGTTCTAAAGCAAGTGACTACATCTCAGGAGCAATAATTCCTGTTGATGGTGGTTTCTTATGTCGTTAAGTGCTTTAGATTTAATTAAAAAAGAAAAACTAATTGCCGTAATAAGATATAACTCTTATGAAGAAGCAAAAAAAGCTATTGAAACATGCATAAAAGGTGGAATTAAAATAATAGAAATTACTTTAACCACACCTGAAGCTTTGAAGCTTATTAAAGAATTTTCTAAATCAAAAGTCTTAATTGGAGCTGGGACAGTTGTAAGTCATGAAGATGCCTATAAATCAGTAAAAAGTGGAGCTAAATTTTTAGTTAGTCCACACTTTAGTGATTCAGTCAACGAAGTTTCGCAAATATCAGAGATACCTTATTTAGCTGGTGTTATGACGCCAACAGAAGCAATAAGTGCTTATAGACAAGGTAGTAAATTACTAAAAGTTTTTCCAAGTGAAATTGTTGGTCCAAATTTTATAAGCGGTCTACTTAAACCTTTTCCATTTTTGGAATTGATGCCAAGTGGAGGAGTAAATTTGGATAATGCAGCAGCTTGACTAAAATCAGGAGCTAAAGCAATAAGTGTTGGCTCTAACTTGTTAGCTGGACAAAGTGAAAAAGAAAAACTTGAAAGTATTAAGAAATATTTAAAAATAGTAAATGGCTAAAATATTATGTATCGGCGAAACTTTAATAAGACATCAAAAAGATTATTACAACAGTCAAAGCATAATTAAATATTATGTTGGCGGAGACGCTTTAAATGTAGCAACTAGTTTATCAATATTAGGCAATGATGTTGAATATTTAAGTGTTTTAAATAAAGAAAGTTTTTATTATCAAGACATTTTAAACCACTTGAATAAATACAATATTAAACAAAATTTTATGATTTATGAAAATCAAAGAATTGGTCTCTATAATGTTATCAAAGGCAATGAATTTATTAGCAGCAAAGTAATTTATGATCGTGATAATTCATGCTATAAATTAATCAAAGATTTTCAAAATTTTAATGACAAATTGCTAGCGAATATTGATTATATTTATCTCAGCGGAATAACAATTTCGATTAATAAAAAATTAAATTCTTATATTTTAGAAATATTAAAAAAAGCTAAAAAATTAAACAAAAAAATTGTTATAGATATCAATTATCGACCAACTTTATGAAATAACAAATATGCTGATTTTAAAAAAGAAATTGATCCATTTTTAAATTTGTCTGATATTGTTTTTGGTTGAATTTCAGAACAAAATTATTGCAAAAGTAATAGTTCAATAAGTGCAAAAGATTTTGACAAATTAACCTGTTTAATAGATAAAAAATACAAAAATATCGAATTGATAATTTCACCAAACAAGGTTATTAGCCAACAAATTGCTAATATAAAATCCTTAGCTATTTCTAATAAAAAAATATATGAATCTGAATCAATAAAATATCAACCAATTTATCCAATTGGGTCGGGCGATTCCTTTGTGGCAGCTTTTCTAAATTATTATGTCAACAATAAAAAAATAAAAGAATGTTTAGATTTTGCAACTAAAGCATATGCATTAAAAAATTATTTTGAATATGACTTTAATCCTTGCAATCAAGAACAAATAAATAATGTAAGTAATGAATCTTTTGAACTTGAAAGGTAAATATGAAATTAAAGAAAATAATATTGCCATTATCAATGAGTGCAGCAATAATGCCTGCACTAAGTTCTTCATGCACTAATTTAAAATCTGAAGGCTTTGATAAATATGGAAATAGAATACCTAGCGAAATTAATCAATTAAAAAACTATTTAATTTTTAAAGAAAGAAAAGTTGCTATTGAAAACACAAAAGCAAGTGTTGCAGCCAATGCAATTAACAATGTTGAAAATGAAGATAGATATAATCAAATACTTTCTTTCTTAGAACCTGAATTTGAAATATTACCTAAAAATTTTGAGTATCAATATAGTGCAAAAGTAATCAATAAATCAGAAGGCGATTTATTAGAAATTAATATTTTAGTAATTCAAACAAGCACAGGCTCAAAAGAAAATATTAAATTAACTTTAGGTCAAGATACTAAATTTAAAAAAGATTCTAACTTATCAAAAAGTGATTATGAGCAAATATTAAAAAACATTTTAATTGTTAATGATCTTAAATATTTAGTCGAAGAAGATGAATCAAAACTTAGCGATAATGATAAAAAGAATAAAAATAATGCAGTTAGATTTACCGATATTATCTTAAACAAATATAATGAAAATCTTTTTATGGATCATCCTCTTGCTAACTTATATTATGCTGGGACATATAATAATGGAACAGGAATAAAAGCTACTGACTCAAACGAAGTAAAAGCTAAAAAAATTTTTGATCGAGAATTAAAAGCAATAGAAGAGGCTAAAGAATTAATTTATAGAAAAGAAAGTCAACCTGTTCAAGATCCTGAAACAAAAAGAATAGTAGATGAAGGCTATAGTGATGCTCAAAAAGTTAGAGTAGCAATTCAAAAATTACTATTTTTAGCTGCTGCGTATCAAATAAAACCAAAAGGAATATTTACTAATAAGTTTTATCAAAATGCAAAATTAAAAAATATTATTGAGAATGTTTTAGATGATTTTGCAAAAAATTATTATTACAGCGGACAACAACAATATGTTAACTGATGATTTTATGAAATCGGTATTCCTAAGGATTTAACAAAATTATTAGCAGTTTTAAATAACGTTTTTCCTAAGGAAAAAATAGAAAAATGAAAAGCTGGTGTTGATTACTTTTTGCCAAATCCTCGCTATGGTGGGGCAGCACCAACAGCTATTCTAGCTTATAAACCAGTTACTAAAAAAAGAGCTCAAACAGGGGCTAATGCTGTAGATAATGCTAAGCCAATTTTAATTTCAGGTATTCTAAATGAAGACACTTCAAAAATTGAAGATTGTATTCTTTCTTTATATGACAATATCTTTCGAGATTTTGTTAAAAAAGGTGACGGTTTTTATGTTGATGGATCATATATGCAACATACAAACATACCTTATGTAGGAAGTTATGGTGAAGTTTTATTCACTAATATTGCAGATATATTCAAATATTTTGAAAATACAGATTTGGCTTTAGGCAAAGATAAAAGATTTGAAAAAATTTATCAATTTATTGAACTCTCGATTATGCCTTTTATGTTTAAAGGCGCAATAAGTGATGGTCTTAGTGGTAGAAGTATTGTTCGTGATGATCACTCAGATCTCAAAAAAGGTTTAAACATTTTAGGCTCTTTAGCAATTATTGAAAAATCTGCTTCTCCTATATATAAAAGTAGATTAAGAGAATTTATTAAAAATCAAATATCTCATTTAAATAAAACTCAACTACAAAAATTAGCATCAGAACACAAAGTTAGACCATTATTAATAGATGCTTTATTAGAAATTAAAGACTTACCAGATTTACCACATCCTCCTAGAAATAAGGAAGATTGAGAATTTTATGAAAATAATTATTTTGATCAAAGAAAAGAACCAAGCTCAACAAATGAGCATTCAGAAAGCTCAGACAATGCTTTAGTTGGTGTTGATTTACATAAAGAAAACGATGGTTTAGTATTTACAAAAAATCAAGATAGATATGTTTACAAACATGATGATTTTATGTTCAATATTAATCTTCATGGAAGAATTAGTGGTTTTCCTGAAGCCTCTGTTCAAGAAAATATGGATTCTTATTATCAATATGATGGCTCAACTTTTTTAGCAACTAATAATAACTATTCGCCTTATTCGAACAAATATTGACAAGCAGTTAATACATTCAAAATTCCTGGCACAACTTCATACATCTCCTCTGCTTACGATAATATATATGATTATATTTTTCCATCTTATATGCCAGTCGATATTGAAAAATGAAAAGAAATAGGTGAGGTCGAAATCAAAGATAAAGAAGGAAATCCAATTCTTGATGCAAATGGTAAACCAGTTAAAAAAACAGGCAAAGAACTTTATGATGAGTATCAAAAATTTATTAAAGAAGATTTGAAACAAAAAGAAAGAGACATTGAATTTCAAGTTTCAACAAAAACTAATAAATCATTTAATAATGGAATAATTTGAAACAAAATGGGTTTTGTTAAGGCAGAAGTAATTAACTACAACAATACCTTAATTACTGATAAAGTTTATTTTATGGTTAATGGAATAATAATAGTTATTGGAAACACAAATATTAAAAATAAGCTAGTTAATACAACGATTGAAAATAGAATGATCATTGATAAAAACACTGATATGTTTAGTAACCAAAAAGTAAACATAAATGGAAAGGAACAAGATTTATTTATTTGAAAAAATAAATTAAATGAACAAAATGGATATTTCATATTAGGAAATAATAAGGCAAAAGTTGAATATTTGCATGAAGCTAAATATCCTATTATTACAAATATGAATAGTAAAAAAATGGTTCCAGTTGTTCGTGATTTTGCTTCTTTATCAATTAATCATGAACAAAATAATACTTTCATGTATGCAATTGTTCCAAACTATGAAGACAGTAAAAAATCAAATTATTTAGATATTTTAAACAACATCAAAATTATCAAGAATGATCAAAATTTCATAGCTTTAAAATATCAAAATAAAGATTCCTTAGGCCAAATTAATGATTATTATTTTGTTGCTAGTTTATATGAAAATGGAAACATTAAATATAAAGAACAACTAAACTCATTAGGAAGTTATGATAATGAAGTTTCAATAAATTACAATGAAATTCCTGAATTTGAAAGAAACTATAAAGCTTATAAAAATGGTCTTTACCTTGAAGATTTAGATCTAAAAATTAATTTATGAAACCCTACTACAATGATTATTAAGAAAGTTCAAAATTCTTCAATTTTAGATGTTTTAGTTTCAAATGATTTAAATGAAAATCAATATGAAATTGAATTTTCAAAAGCATTCCAAAATATTACTTCACGTAAAATAACAAACAAAAAATTTAGTGCAAAAAATGTTATCAATTTAAGCGAAGTAACTTTAAATAATGTTTTAGGTCAATCAAAAATTAGAGTTGATAATCGATTAGGAGAAGTTAATGATTATAATCATATTTCATGATTTACATTAACAAATTAAAGGAGTTATTATGAAAAAACATAAAATTATATTTGCTTTAACTCCATTCACTCTCATGCCTTTAACTGCAATAAGTTGTAGTATTGAGAGTTCAAATGATTATGAAAACGCTAATAAGTGATCTAAAGAATCAAATAAACCTGGAATGAAAGAATCTGAAAACATATTAATCAATAAATTAATTAAAAGTGAATTTAAGATTACTAATTTTTATAATGAAATGATTCTTAATAAAGAATTTAATATTAATGAAGTTGGTTCAAAAATAACTTACAATAAAAATTTTAAAGCATTTAATAATTTAGATGAAACAACTACAAATTTTGACCAAAATTCTTTTAAAACAGGCAAAATAGTCAATTTAAAAAAATATTTTACTTGAAATAAAGGTGTGCAATATTTGCCTTCATGAATACAATATGATGTGCAATCTTACTTCAATCCATTAAGTTCAAAATTTGCAGATTATGTTACAGAAGATACATTGTATTTAAAACTAACGCTATTCAATTCTTGTGCTTTGAATCTTAAAACTCAACACATAATTTATGCTTTTACAAACTCAAACAAAACAGGTAGATTAATTACTAAAGATAATACTTTAGAACTTCAAAAACTAACACAAATAAGTTAGTTAGAAAGGAAACAATGAAAAAAATTAAAATGATTTTAACGCTTAGTTCTCTTACAACTTGTTTACCAATAATTGCTACTAGTTGTAAAAATGAAAAAGAAGAAAAAATTACAAATTTTGATGAAGTTAAATCAAAATTAACTTTTGATGTTGATAATAAATCAAATATTAAAGCATCAGAATTTAAAGATACAAATCTTATTAAAATTAATAACTTACCATCTGATTTTAAAAAAGAAATTACTTTGGAAAATTATGAAGATGGAAAAATTAAATTTAAATTAATATTAATTGACAAAAATAATAAAAAATCTCAAGCATATACTATTACTATTTCAGGTTTTAAAGCAGAAACTGCTACACCTACAATTAATTGAGATGAAGAAAAAGGAAAAGTTTCTTTTGAAATAGCAAATCTAAAAAGAAAACCAAGTACAGTAATAGAAAGTGAAATCGAAATAAAAAGACCTCAATTAGATTCTTCAATAAAAATTAATTTAACTTTAGAACCAAATGATGCTGAGAAAAAATTAGTTATTAAATGAAAAATGACAAAAGACACTTCAAATTCACCAGTATATACTTTTGAAATATCTAATTTTGAATTTACAGAAGAATTTGACTTCGTTAATTTTGATGAAGTTGCAAGCAAATATAAAGAACTAAAATTACTTACTGCAAACAAAGCAGAAGCTAAAAATGTTATAGCTGCATACAATGCAACAAAATCATCAAGTCTTGATGCAAAAATCAAAGATAATATTTTAGTTTTAATGGCTGGTATTTTTGCTAAAGGCAAAAACTCAAGTAAGCCTCAAGCAAGTTGATTTAATAAATCAATTAAACTTCTTGAAGCTGAAAGTAAAACTAATTCACATATTGAAGATATATATCCAGCTGTTCAATCACTTAATTCAATTTTAGCTTCTTATGATCAATTAGAAAATGATGAAAAAACTAAAGCTGATGAAATTATTCCTGCAACAATAAATGCTTTAGCTGAAAAATATTTTGGAGCTAACCAAGTTATTTATAAAGAACATTATTTCTATCAAGAAAAAATGCTTGAATTAATTGCTAGAACTTTTGCCACTTTAAGTGATACACAAAAAACTGCAAATAAAACTAAATTAGACATTCTTTATAATGCAACAAAAATATTCTTACCAAAAGCTTCAAACTTAAATTCATTTAAAGGTGAAGATGCAAACTTGTCAGATGGCAAAAATTACAAACCTACATTTAACCGTTGAGCTAATAAAATGTATTCAATAACTGTTTCTAAATATGTTATAGCTTTAGCTAATAAAGATAAAGACGAAGCAAAAAACGTTCTAAAAGATTTTTGAGAATGAACAAAAGATAAAAATGCAGCAAATATTTCATTAAAAGAAAAATCAAGTTATTACTTTAATTTTGACAATAAAGACAATAACAAGAGATTGTATAATTTTTCAAAACAATTCGATATGCTTAGAAATATCAATAGATTGTTAACTAAAAATGTTAAGAATACTGAATGAGGTACATTATTTGATTCTATTAACAATTACATTAATAATGAATTAATTGACTGAATCAAAGATACTTCAAGCTTAGTTAAACTTTCAACAGGAATTCAATTAATTGGCTCATTTATTAAATTCTTAGAAACTGAAAAAGATAATGTAAGATTAGAAACACTAAAAACTAATCTATTAGCAAAAGAATTAAAAAATATAACAGGCTATGATCAAAAACAAGGCGGTTGATCATGAGCTTGAGATGCAGTACATAATTTACTTGCTTAAAATATTTTAATATCACATTCATTTGTGATATTTTTTTAAACTTTTATTATAAAAAACCAGCCTTTACTAAAAGCTGGATCATAATATTAAAAACTAACTAATTTTCTTTTTAACTAATTCAAAATATACACGAATAATTTTCGATAGTATTCTCTTTTACTACTGCTTAAGTCTGAAGGTAAGTTATTAGTTGCTTTTTGAGTTATGCTTTCATATATTTTTTCTTGTTCTAATATTTTTTATCAATACAACTTAGAATTGAGAATCAATCCTCCATACTTAAACTTTGAGCATAGATTTCACTTGGTTTTTTTGTTATTTTTAAAGTTCTTTCTTTTTGTTTAATTTACTAAAAATATATTAGTTTATAAAACTAAAAAATAAAAACTTAATTAAATTTTTTAGTAAGTAATCACAGAAATTACTGCATTATTTCTTAACTTTAATTTATAATTAAATAACTAAAAAATAATAAGGATGGTCCAAATGACAAGCAAAAAAATGGATAAATACATTGCTAGTTATTTTTACAAAACTGAAAAGATATTAGCAAAATATAATAAAAATAATGTAATTGTTTTACAATTTTTCCAACGCCGTGATAATAGCATGCTAGCTGGTATGCAAGAAGTATTAGATTTATTAGAAAAGAATACTGATACTTCTAAATACATTATTAGATATTTACCTGATGGCACAATTATTAATAATTTAGAAGTAGCTTTAGAATTAGAAGGTCATTATCAAGACTTCGGTAAATTCGAAGGAATGATTGATGGAATTTTAGCTCGTTCAAGCTCAATTGCTTCAAATGCTTATGATTGTGTCCAAGCTGCTAAAGGCAAAAATGTAATTTTTATGGGAGATCGAGCTGATCACTATTTAATGCAAGAAATAGACGGAAGAGCTGTTGCTTTAGCTGGAATTAAATCAGTTTCAACTGATGCTCAAAATATTCATAATTATGAATTAACCTTTGGATCAGTACCTCACATTTTAATTCAAAACTTTGATGGTGATACAGCTAAAGCTATGCAAGCTTATGGCGAATTATTCCCTAAAAATAAAATCATTTCTTTAGTTGACTACCACAATAATGTTATTACTCAAGCATTAGAATCATATAAAGTATTAGGCAAAAAACTTTGAGGTGTTAGAATTGATACTTCTAAAAATATGGTTGACCATATGTTTGATAATGAACCTGAAAATCCAGAACATTATGGTGTAAATATTGAACAAGTTAAAAACTTGAGAAAGGCATTAGATCAAGCTGGTGCTAAGGATGTTAAAATTGTCGTAAGTTCAGGATTTAATGCTGAAAAAATTAGAAAATTTGAAGCAGCAAATACACCGGTAGATAGTTATGGTGTAGGTCAAAGTATCTTTAAATTAGTTTCAAGTTTTAGTGCTGATGCTGTACTTTTAAATGGTAAAAAACAAGCTAAAGAAGGACGTGTTTATAGACCTAATCCAAAACTTTTAGTTTACAAATCAAAAAGAAAATAATTTTTACTTAAATATCTATAAAACAGGCTTATTGGTCTGTTTTTAAATTTAATTTTTATTAAAAAATGTTAAATAATTTAATAATAAAGTATAATTTAAATATAATTTTTTTGAGAAGGGAGCTTGTAATGAAAAAACAATCTACAGCTTTTGTTGCCGTTGCTTTATTGCAAACATCAATTATTATCATTCTATTTATTTTAGGAATGATTGAAGCAATTAATATAAATGGAGCTTCGCTTAGAATAGGCATCTATGGTGCAGTTGGATTTACTTTAGTTACACAAATAGTATTATTATTCTTTGCATTTGTTTACAATAAACCAGGATACAATGGTAAATTGGGAATATTATTAATTGTTTTCTTATTCTTGCTTTTAGCTGCATCAATTGTTTCATTATCATACACAATTTGTTCAACAGAAGGAGCTAACATTAATAATGATGGCTATAAAGTGTTTGGTATTATTTCAACAATATTCACTTGAGTTTTAGCAACAATATTCCTTATTTGTACAATTGTCTATGCAGTTAGATCTAAATAATAACTAAGCTTGCAAGTGCAAGCTTTTTTATATTCTTTTTCTTAGAAAAATAAAGCTTTCTAGTATAATTTTGATACATATTTTAAAACTAAATGAAAAAGGAGAATTTATGATTAATGTTAACACATTCAAGCCAGGAATTACATTTGAAGATGAAGGCGAAATTTTTGTTGTATTAGAAGCTCAACACTCAAAACAAGGTCGTGGACAAGCTAACGTTAAAGCTAAAGTCAAAAACTTAAGAACAGGTTCAATTACTATTAAATCATACACAGGTGGTGTTATGGTTTCTAAAGCTCACATTGACAAACGTCCAATGAATTACTTATATACAGATGGTAACAACATTATCTTAATGGACAATGAAACCTATGAACAAATTGAAGTACCTCTTAGTCATGTTGAATGAGAAATGAACTTCATTAAAGAAGGATCAACAGTTAAGGTGCGTATGTATAAAGATGAAATTTTGGACATTGAAATTGATGATACAGTTACTTTAGAAGTTATTGAAGCACCTGATGCAGTTAAAGGCAATACAACAACAAATCCTCAAAAGAAAGTTAAAGTTGAAACAGGTTATGAATTAGAAACACCTATGTTTATTAAAGAAGGTGATTTAATTGTTATTTCAACTGACACTGGCAAATATAATGGTAAAGGAAAATAATAATGAATTTAGTTACAACAAATTGTGGTTTAAATCTTTCTTATTCAATTACAGAAAACGTTTTTAGTCAATTGATTGATCAATGCTTTGAAGAAGTTAAAAATGTTAAATTAGCTAATGAAGCAAGAATTTCATTTGATAAAGAACAAACAAATGTTGCTTTTGTAATTGATATTAAAATTAAAAAAGGTAGTGGTCTCCAACAAGTTATTGAAAATTTAACTCAAGAATTAGAATCTCGCTGCTTAGGTTTATTAGATATTAAACCTCGTAGCATTAGACTATGCTTTGTTGGATATTATTAATAATAAGAAGTTCTCAGTAAGGGAGCTTTTTTATTTTGCTTTGATGATGCAAAATAAATGTTAAATAAAAAATAAGAGCAATTAAGCCCTTATCAACTTTTTAATTAATACAATTTCACACTAACATCTTGTCTATCAGCTTCTTTAGCTTGGAATAATAGAATTGTTGTTAATCTCATACTTGAAGCAACAACACTTTTTGGATATGTAAAGATTTCTTGGTTAAATGTTGTGGCTGAAGTATTGTAAATTCTTCTAGCTGAAGCAATGTTGTTTTCACATTCAACGATTTCATCATTTAAACCTTTAACTAATTCAGCACTCTTAAGTTCTGGATATTTTTCGAATTGAGCCATAAATCTTGTTAAGAATGATTCTGTTTGACTTTCAAAGTTTTGACGTACTGCCATAGCATCTGCTGAAGTATTTCCTGATTGTTCTCGAATTGTAGCAACTGCTGAACGGTATGCTGCAACTTGTGTCATAACATCTTTTTCGAATTTCATGTGTGAACTTACTGATTCAACTAACTTAAGAATTGTTCCTGCTCTTTTAGCCAATTGAGTATCAATTAATGAAGCATCGCTACTTATTTTCATTTGTTGTCTTCTAAAATAATTTACTCAAGAAATATGAATAAAAATTGGAATTAAGAAAATAAATGATATGTATCAAATAAATTTTCCAAAAAAGTTTACTTTAACTGGTACTTCTGTACTCATTGAACGTGGTTCATAGCCTTTTTCATTAATAGCACGACTGTTTTCACGATTGTATAAATTAGCCATTTTTTCTCCTTCTATATAATTATTTTTGTGTTCAATACATAAAAATTATATAAGTTTTTATAGTTATTAAAAACAAAAAGTTAAGCTATGCCAGCTTAACTCGTTTTTTAGATTCATATTGATAATCAGTGGCAAATAAATAACCATCTTTAAATTTTAATTTAGGTACATTTTTAATTTCTTTTTTGGTTGCAAAAAAGATCTTAATTCTTTTATTATCAATAAAAGTGTAAGCACCAGGATTCATCTCATAAGCTCTAATAATATTGATTGCTTCTTGCATGGTTAAGGACTCATTTAAAAGAGCTTTTTCTTTTTCTAATTTGGGACTTAAAGTTACTTTACTTTCATCTTGTTTAATTCTTTTAAGTTTGTTTTGATCTAAATCATTTAATCATTGCACAATGTTATCTGCACTAAGCTTTGAAAGCTTATTAAATAAAGTACTTGCTGTGTCATATTCATCTATTTTAATGGCTTTTTGGACAAAAACATCACCTGCATCCATTTGTTTAATCATTTCCATTAGACTAATACCAGTTTCTTGATCATTATTTAAAAGTGAATATTGAATTGGTGCTGCTCCACGATATTTTGGAAGTAAAGAACCATGAATATTTAGACTTAATTTTTTAGCAATATTTAAAACACTTTCAGGAATATATTGACCAAAGGCACAGGTAATTAAATAGTCATAATTTAAAGTTTGTAATTCATCTTTTATTTGTGATATTTTTTCTGGTTGAAAACATTTAATGTTATATTTTTGAGCTAATAATTTTGTTGGCGTTGGAGCTAATTTATGACCTCTGACACTTGGGCGATCAGGTTGCGAAACAATTGCAACAACTTCAAAGTTGTTGATTAATTTTTCAAATATTTCAACTGCAAAAGCAGGAGTTCCGGCTAATAATAATTTCATATTTTAATTATATAAGGAAAAATAAAAAGTGGAATTAATCCACTTATTTTAATTATTCTTTTGTTCTATGTTCAATGGTTGCTGGTTGATTTGCACTTTTTAAGAATAAATGTAAGAATGTTGAAACTAATGTTACTGCAAATGTTACAAATAAAAAGAATGCAAATGCAACGTGAACGTTAGCATATGATTTATCATCTAATGATCTTGGTCCTGTTACACTTCAAATATTCGCAATAATTCAGCCAACATAACAAATAATTGTTAAAACAACTGCCGCAAATACCAAAATATTTAAAATAATGCTAATTACATTTTTCGAAGCTATTGATTTTACAAATGCTTTAATTGCATAAACAAGAATAGCAATCATTGTAATATGAATGATTATATGTGCTAATAATGAAACAACATAAATAATTTTTACATTATTGCTATCATGTTCAAATATACCTTTAATCACATCAAAATTTTTAAATCATAAGAAGAATCCATAATTTAATCCAGAATGATATATTGCTTCGTATTCATTAAAACTAGATTTTGTGTTTTTTACGATAATTTGATGCAAGTCAACAGCTGATTTTACTATTAAACCAAAGATAACTAAACATGAAATTGCTAAACCAAATAGAATTCATGATGTTTTAGTTAATTTTGTTCTTTTTGTGTTTTCCATTTAATGCCTCCATATAAAAATTGTATAAGCAAAAATTTGAAAATAAAAACAAAAAAATAAAAATAATTTTGACTAAAATGTATATAAAACAAGTAAATAATAGAATTATTTAATAAAAAATAAGGCTTTTTAAAACCTTATAATTTTTTTAAGCTACAACCTTTTTACGACGTTTAACAATAAATCTTCAAAGAGCAACTAGAGCTAAAGCAAGAGGTAAAAGAATTGTTAAAGCAACTACTACTAGATAAATTCTAAGTCACATTTTGTTACGATCTTTTTTGCTTTGCTCAACTTGTTCATGAATTTCAACAGAACGTTCACCGAGAAAATCACTAATGTCAGCTAAACGTTTTTTATCAAATAATCTTATTAAAATATACGCCGCTATTGAAGAAGCCATAATAATAACACTAATAATACAGAATAGAATTGTGTTGCCAAAACCTCTATCAAGTCATTTAGCTCATTCAAAATTGATTTTTAATGTATCAGTTTCATAATATCAACGATTTGACATATAAAGTCCATAAACTATTGCTGTAATAATGCCTAGATATGTTAAGACAAAAACAAGCATTCAAGTTAAATTAACATCTTTAAGTACTATCTTACGATAAGCAATATGAAAAGTACTACTTGAAGTGTAATCACCATTACTTACTGCATCACGATATCTTTGAACTGTGTGAGATCATTGAATATTTTCAATCAGATTTTTAAAACCAATTGCCATTGAGATAAAGGCACTTGATCCAAAAAGTACAAGGTAGCCAACTAATCTTTGTTCTTTGTTGAAGATACCAACTTTAGCATAGACTAATGTGAAGAGAATAATTGAAGCAGCCATAATTACAAATGAAATAGAAAGGATTAAAATCTTAAGCATTTGTTCAGCACGGATGACGCGATAAACACGAGGTGATATTTGATTTTTTGGATCACGATAAACACGACTACTTACACTATTGAATTTGTAGTCGTGGTTATTTTGAACAATTTCGATACTTGCTGAATTTTGAATAGGTTGATATTTTCTCATTCTTGGCCTTTCAATTTTAGTACTCTAATTATAAATGATTATTCGCTATATTTATTAATATAAATTTTAATATTTTATAATTATTTTCAATAATTAAATTTGTGAGATTTTTCTCAACAATTTTTCATTTAAAATTGATGGATTTTACTAATAAAGCTTTTTTATAGATTGATTGGGCAAATATATAAAAATTGTGCCTATGATTAAGCACAATAATTATTTGATTATTATTTGTTTTTGTTTGAAGGTTCTAATTCTTCTTTTTGTTCGTCTGCAACAAGTTTTTTAGCATCTGCTAATTGAATGCTGAAGAAGCTTGTAACACCACGGTTTTGCATTGTTGCACCAAATAATGTGTCAACTCTTGACATTGTTCCATGACGGTGTGTAATAACCAAGAATTGAGTTTTTTCTTTAAGTTGTTGAAGGTATTCTGCATAACGAACAACATTAGCTTCGTCAAGTGCAGCTTCAACTTCGTCAAGGATACAAAGAGGTAATGGACGAGCTTTAAGAATACCAAATAATAATGAGATAGCGATAAGTGATTTTTCACCGCCTGAGAATAATTTAAGGTTTTTAATATTTTTTCCAGGAGGTTGAGCTTCGATTTCAACACCTGATTCTAAAATATCTTTAGGGTTGCTGAAAAATAATTTTGCAGTACCACCACCGAACATTGTTCTAAAGACATTGTTAAATTCATCATTAACATCATTAATAATGTTAGTTAGACGAGTGATAATGATTTTATCCATGTCTGCAATAGCGCTTTCAAGTGTTTCTTTTGCAGCACTTAATTCTCTTTCACCTTCGGCATATTCATTGTAACGTTTTTCTTCTTCAACTAATTGTTCAAGAGCTTCTAAGTTAACGTTACCTAAGCCTTCAATTTCTTCTCTTAATTGACGAACAATTTCTTCAGCTTGATCACGATTCATTTCTAAGTGATGAACTTTTTCAGCTGCTTCAAATGTCATTTTGTATTGAGAAGCAAGTCTTTCTCTACTTTGTTCAAGTAAAGTTTTTGATCTTTCTCTTTCACCAATTTTAGCTGAGAAAGCATCTGTTAATTTACGACGTGTAGCTTCATAATCATTTTTAAGAGCTAAAGTTGAATTGATTTCGGCAGCAATTGTATTTACACGTTCATTTTTAGCTCTGAATTGTGATTCAAGAGCAATTTTTTGACTTTCTAAAACTTCAATTGAAGCTTCAGGGCTTGATGCTGAAACACCTTCTTTAACTTTGAATTCTTTGTTTGTAATATTTTGAAGTTTTAATTGTAATTCGTCGTAAAGACCTTGTTCACTGCTCTTTTGGGTTTTTAGACTAGTAATTTGTTGTTTTAATTGGCTGTCTAATTCAAGAGCACTACGACGATAGTTTCTTATTTCATTGATTTTTGATTCATCAGCTTGAACTGCTGCTCTCATACCTGTAACAATATTTTCAAGCTCTTTAATTTTTTCATCAAATCCAAGTAATGATTGAGCTGTATTTTTAGCTCCACCAATAATCACACCGCCTGAACGAATGGTGTCACCATCAAGTGAAACTACCATGTAACGACGTTCGAGAATACTTGAAATTTTGTTAGCATTTTCAATGTCGCTAGCAACAATAACATTACCTAAAAGGAATTTATTAAGAATTTCATATTGAGGATCAATTGTTACTAAATTACTTGCAACATCAATAAATCCAGGGTGTCCTTGGATTGCTAAAAGATGATCATCACGAATCATTTTTGGTTGAATTGATGTGAGTGGAATAAATGTAGCACGACCACCATTATTATCTTTTAAGAAGTTAACAGCTTTAACTGCTGTTTCAGCTTTATCAACCACTAAGTGTTGTGAAGCATTAGCTAAAATTGAGTCAATAGCAGGAATATATTCTGTTGTAACTTTAATTAAATCAGCTACAGTTCCTTTTAGTGCTTTTCCAAAGTAAGCTTTATTTTCTAAAATGTTTTTGGTCCCTTTAAATAATAAAGAGTTACTTTCTTTTTGTTGGTTAAGCAAGTTTAACTGTGTTTCAATTTTTAATAAGTCTGTACGTTTTTTGTTAATTTTAATGTTCATTAAATTAATTTCTTCGTCATATTTTGAAATTGTTTTTTGGTTTTCAGCAATTTGGTTTGCTTGATTTTCAATTTCACGAGCGTAGTAATTTAATTTATTTGCATGTCTTGTAAGCATAGTTTTGTATGCTTCGATTTGTTCAGCTTGGTTTGCTTGAATTTCACCAGCAGCAATTAATCTTTCACGTTCATTTTCTTTAGCTAAAGAAATGTTTAATTTGTTTAATCTTTCTTTAACTGTTTCATATTCAATTGAAAGTTCTTTTAATTCGGCTTCAACTTCTGATCTGAATTTAGTATTTTGAGTAATAGCAGCTTCAGCTGATGAAATACGAGCATCAAGATCATTTTTAGTTTCTTGAACACCTTCAAGTTCAGTTACAAGTCTTTCGTACATATCACCAAAAGTTTTGATGTTGTCTACTAAAAGACCAACTTCAACAGCTTTTAATTCTTCACTTTTAGCAATGTAAACTTTAGCTTTTTCTGCTTGTTTTCTTAATGGAATTAATTTTCTTTCAATTTCACTAATAACAAGTTTAATTTGGTCTAAACCTTCTTGAGTTTTAGCCAATTTGCTTAAAGCTTCTTTTTTACGAAATTTGTATTTTGAAACACCAGCAGCTTCTTCAAAAATAGACTTTCTTTCTTCAGGTGTAGCTTCAGCAATATCACTAACTGTACCTTGAGAAATAATAGCTAATGAACTTTTACCAATACCGCTTTCCATAGCAATATCTTTAATATCTTTTTGACGACATGGTTCGCCATTTAAGAAATATTGGTTGTTTCCATTTCCTCTTTCAAGCACACGACTAATTGAAATAGTTTCATGCGGGATTGAACTAATACCATTTCGATTATCAAAAGTTAAAGTAACTACTGCTTTATCCATAGGTTTTGCAGTTTTAGAACCAGCGAAGATAACATCATCCATGTTATCACCACGAAGTTCTTTAGCACTACGTTCACCTAAAACTCATTTGATAGCATCGTTAATATTTGATTTACCAGAACCATTAGGGCCAACAATTCCGGCTACTCCACCATCAAATTTAAGCACAATAGGATCAGCAAATGATTTGAATCCGTGGGCTTCAACTTTAATGAGTTTCATATTACCTCCTTTATTTTTTGAACCACTCAATTCTTGATTTTTTGACGGTTCATAATTATAATTTTAATATTAATATCTAATTTAATTGTAAAAATTTTTATTTTTTCATTTTATGCAATGCATCTAGTGCAGCATTTTCTTCGGCTTCAGCTTTGCTTTTACCAAAACCAATACCATAAATTTTATTGTCGTGAACTGCGTGAGCTTCAAATTCAGTTGGACTTTTTTCACTAACTATATACATAACAGCTAGTTTACTGAATGATTGAATTTGTTCTTGAAAAGCAGTTTTAGCATCTTTAAGATGCTTGATGTCAAAGTCTCCAATTTTGATATCAAAAACAGTTTTGTTTAAAAATTCTTTGACTACTTTTAGATTTGTATCCAAATAAATAGCAGCAATAAAGGCTTCAAAAATGTCAGCGCCAACTTTAACTGAGCGTTTAGCATCTTTACTCATTTGACCAGGTGCAGTTAGTAAAAAATCTTTTAATCCAATTTTAGTGCTAATTTCATTTAGTGACTTTGTGCAAACTAATTTAGAACGAATTAAAGTCATATTACCAGCACTTAAATTTGGAAATTTTTTGAATACAAAATCTGACGAAAGATACTGTAAAATAGCGTCTCCGAGAAATTCAAGATCTTGATAATTGCCAACTCTTTCACTCACATTATTTTTGTTTTGATTTTTTTTGAGTCCAAAATATGAACCGTGAGTTACAGCTTGACGATAGTATCCTAAATTGTGATAATTGATGTTTCAAGCTTTTAAAAATTCTTGAATAGTTTGTTTAATTTCATCCATTATTTATTATTTTTCTTTCTACTCTTCATTAATATTTAAGTCTTTTTTAACCTCAGATAAAATGTGGTTATCTAAAGCTAATTTTATTTGATTAAGTGCTCCATAGTAAGCTTTTTCATCACTACTTCCATGGCTTTTAATTACAACACCATTAACCCCAGCGATTCAAGCAGCTCCAACATTACGATAATCAAGAGTTTCAGCTACATCTTTAAATGCACCACGAGCTAAAAGGTAACCTAATTTTCTAATTGGTTTCAATTTAATTTTGCTAGTTAATAAATTTTTAAAACTTAAAATTGCACCTTCAAGACTCTTAAGAATTAAGTTACCACCATAGCCATCGATTACTGCTACATCACAAACACCATTTAATAAATCACGAGGTTCTTGAAAGCCAATGTAATTTAATTGATGTTGTTCTTTAAGTTTATTATTTGCTTCTTTAATTAATTCTGTTCCTTTATAGTCTTCAGTTCCGATGTTAATTAATGCACAACGAGGTGCTAAGTTTTTTAATAAAACACGATCAAAAGAATTTGCAATTTTAGTTCATTCAACTAAATAATCACTTGTGGTTTCTAAGTTAGCTCCAACATCAAGAAGTAAGAATTTACGTCCTGTGATTGTAGGCATTAAGGGCATAAATGCTGCTCTTGAAATGCCTTCTAATCTTTTAACTTTAAAAGTAGCAGCTGCAAGATATGTGCCAGAGTCTCCTGAAGAAAGTACAGCATCTGCTTTTTTATTTGCTACTAAATCAAGTGCAACATTCATTGAGGTATTTTCTCTTAAAGATTGGCGAATGTTTTTAACATCGCTTGGCACTGATGAGTTGTCAATTATTTCTAAATTTTCTGGCATGTTTTTGCCATAATATTTTTCAATTTCAGCCTTAGGACCAACTAAAATAATTGCATAATTTTTGTTGGTTTTTAAAAATAATTTAGTTGCTGCTAAAGCTGAATTAATGCCGTTGTCATTGCCATTAATATCAAATGCTATTTTGTACATTTTGCTCCTTATTTTATTCAAAACCAATAATGAAGTGGTAGTTAGGTTGAGCACCATTCACTACTTCAACTTCAACATCATAATGGCTTTCTAAAAAGTTTTTAATTTCGTTAGCATCAACTTCACTTGCATCATTTCCATAGTAAACTGTAACAAGTTCATGATCACTGTTAATTGATTTTTTAATCAATTTTTTAGCTGCATCAATGTAGTTATTTTCACTAACTAAAATCTTGCCATCAGTAATGCCAATGAATTGACCTTCTTTGATTTTAACATTGTCAATTTTTGTGCTTCTAATTGCTTGGGTTACTTCAGCTGTGGTTACAAGCTTAATAGATTCAGACATTGTTTCACGGTTTTCTTTAGGACTTGATTCAGAATTAAAGTTCATTAAAGCTGTTAATCCTTGAATTTGACTTCTAGTTTGAATAATAAATACCTTTTTATTTGTAATAGCTTGAGCTGCTTGTTCAGCTGCTAGGAAAATGTTTGAGTTATTAGGTAAAATGAAAACGTTTTTGCTATTTACTGAGTTAATTGCATCAATAATATCTTGAGCTGAAGGGTTTTGTGTCTGACCACTTTCAATAATAGCGTCAACTCCAAATTCTTTCATTCTTGCAATAATTCCACTGCCTAAGTTACATGAAATAATGGCACATTTTTTGTTTGATTGTTCATCATTTTGGTTAATAATAACGTTTTTATTACGACTTTCACTTGCTTGACGAGTCATATTTTCTGACTTTATTTTAATAAATTCACCATATTTTTGACCAAAGTTTAACATATCACCAGGTTTTAATGTATGACCATGAACTTTAACAATTTTTTCATCTTGAACTACAACTAAAGAATTAGCTTTTTTAAGTAATGATTTTTCAAAATTAGCTTTATCAAAGTCTTTTTCGTCATTTAATTCAATAATGAATTCAGTACAATAACCAAATTCGCCATCATAAACTTCTTTTGAGCTGATAAAAGTATCAATGTTTTCTTCTGATTCACTAATTTTAATGGCTTCAGCATTTAATGCTGCTTCCATACCACTAATAATTGTATATAATCCTTCACCACCTGAGTCAGTTACGCCAACTTCACGTAAGATCTTTAATTTGTTAGGAGTATTGTCACATGCTTTACGAGCTGCATTTTTAGCTAATTTGAAAAATTCTTCTAGTGAAGTTTTATTATTTACTTCTTTTTCTAATGCTTCTGTTGTTTCTCTAATAACTGTTAAAATGGTACCTTCAACAGGTTTTAAAACTGATGAATAAGCTTTTTTAGTCGCTTGCTTAAATCCTTCTAAAAGTCCTTTAATGTCAACGCTTTCAACTTCTTTAAACGCAATTGCAAAACCTTTAAAAATTTGGCTTAAAATAACACCTGAGTTTCCGCGAGCTCCTAAAAGCATATTTCTTGAAATAGTGTTAGTTACTTCACCTAAGTGATTTGAGTTATTTAAAGCCAAAGCATCAGCAGCAGCTTGAGCTGTTGATGACATGTTAGTTCCTGTGTCGCCATCTGGAACAGGAAAAACATTTAAAGCGTCGATCTTATTTTTAGAGTTAATAATGTTATTAGCTCCGGAAATAAAAAGTTGACAAAAAAGCTTACCGTCTAAAATCTTAGTCATTTGCAAGACCTCCTATAAATACATTTAATTTGCCTAAATGTTCCTTTTTCTTATTGAGTTCGTATCTTAAGTGAGAACTAATTGAGTTAACAACATTTTTTGCATTAGCATATTTTAAAATAATAATTGTAGCGCTGAAATGCCATTCATTGTTTATATTTTGAATACTTAAATATTCTTGGCCTAAATTAGGACATTGTTCTGCATTTTCACAGATTCCATAAACCCCCGGCGTTGATTCAAGAATATTCAAAAAAACTTTTCTAATTTCTTCTAAAGTCATTCTTACTCCTTGTGATAATTAAAAGATTATTTATAATCAAGTAAAACTATTTTAACAAAAAAGAAAAAATACAATAAATAAATATTAAAATTAATATTAAATATAGTAATTAAATTAATTTATTAGGAGAATTATGGCTGAAAAAATTTTACAAGTTGTTGTTTTAGACATTCAAAATGCTAATACAAGTGATAATGATGCTATTGTTCAAGTTTATTCAAAAAATGGAATTTTCTCACTTTTAGCAAAAGGTGTAAATAAAAGTGAGTCAAAAAATCGAGTTAACTTACAAATAGGATCCTTAGTTGAAATTGAATATTTTCAAGCTCGATTGAACAACAAAGTGAGCTTGCTTAAAAAAGCCACTTTAATTTCAGACATAGATTATTCAAATCGTTTTAATTTAATTTTTATTCAAAAAGCAGTTAATTTATTAAAGCATTTTCCTAAGCCTTATTACACATTATTTGAAACTTATGTTAACTGTTTAAATTACTTAGGATTAGGCAAAAATTCAATGCTTTATACTTATCTTTTAGCTCATACTTTAAACTATTTTGGACTAGATGTAAATGTAAACAAATGTTGTGAATGCAATAGTCCAAGCAACTTGTGTGATTTTAAATTTTATAAAGGTGGATTTTTGTGCGGTAACCACATGAGTGAGCAACGTTGAACCAAAGAATTAAAATCAATTTATTACATGTATAATGATTTAAAAATTTTCTTGCAAATTTGTATTTCAAAAGTAAATCAAACTATTTTTTATGAGCTAAATAAATACCTTGAAGAAAATGGAATTTATCTAAATTAAACTTTTAAATTTTAATGAAAACAACTATAAAACAGGACTTTTGGTAAAAATCTTGTTTTATATTTTTTAATTAATTTAATTTTTTGTAATAATAAAAACATTTTTTAATATAATTAAAATAGTTATTTTTTAAAATGTAATTTTCTGCAAAAAATAACTTGAATTTTAGGAGGTAAAATCGCATGTCTTTTATTGAAAATAATTTAAAAAGAGGATCAACAAGAGATTATCAAATTGGCTATGAAATTAGTGAAGAAGATAAAGAAAAATTGATCAAAACTATTCAAAATGCACCAACAAGTAACAACTATTTTGCTTCTTCAGTTATTGTAATTGAAGACCAAAATACTAAGGACAAATTAGCTGAAATTTTACAACAAAAACAAGTTAGAGAATGCTCAATGTTTTTTATCTTTTTAGCTGACAATAATCGTATTGATTATGTCTTAAAAACTAAAAATAAAAATATTGAAAATAAAACTCTCAACTCATTATTACTTTCAGTTGGAGATGCTTTTATTCAAGCAACAATGCTTCAAGATGCTGCTACTGAATTGGATTTAGGAACATGCTTTATTGGAGGAGTTAGAGGACACATTAGTGAAATCAATAGATTACTTAAAATCAAAAACTCAGCTTTTCCAGCAGTTGCTTTAACAGTTGGTAAAGCAGCTAAAAAAGCAGATCTTAAACCAAAAATTAATAGAATTTACTATGAACAATATTCATATGCACAAGTTGTTGATGAAATTGTTCCATATAATGAAAAATTAACAAACTATTGAAAAAATAAAAATATTGAAGGCGATTATGTCAATGCCAGTGCTAATTACTTAAGTAAAAGAGTTAGCGGCCGTGACAAAACTATTATTAATATTGCAAAAATTAAAAAAATGTAAATAAAGTATTAAAATAATAAAGATTAATTTACATTCACAAACATATATTAAATAAATAAAGGAGAAAAATGGCAAAATTTAAACGTGTTTTTATGATTGTTACAGATGGTCTAGGAATTGGACCAGATAAAGATCAAAGAGCTTTTGGTGACAAAGGCGCTAACACAATTCTTTCAGCTTCAAAAAGCTCAATGTTCTTTATTGACACATGAAAAAAATTAGGAATTGGAAATATCACAACTTTAGAAGGTAATTATCGTTACAAAGATCAAAAAGCTTACATGACAAGAATTCAAGAAGTTTCAAATGCAAAAGATACTCTTGCAGGTCACTGAGAAATGATGGGAATTAAAACAATGGTTCCATTCCCTACATTTACTGAAAATGGTTTCCCTCAAGATTTATTAGATGAATTATCTAAAGCTTTTGATGGTCGTAAAATTATTTGCAACAAATCAGGTTCAGGTACTGAAATGATTGATGAATATGCAGAACAACAAAAGAAAGATGGTTCAATTATTGTTTATACATCAATGGACTCAGTATTACAAATTGCAGCTCATGAAGAATGAATAGGTTTAGACAACTTATATCGTTATGGCAAAGCAGCAAGACAAATTTGTTCAAGCAAACCAGAATGAAATGTTGGACGTATTATTGTTAGACCATTTATTGGTGAAAAAGGAAAATACACAAGAACATTCAACCGACATGACTATGCAAACCAACCTCGTCCAATGATTCTAAATGAATTACAAAAAGCTGGTGTTAATGTTATTGGTATTGGTAAAATCTTTGACATTTTCGTAGGTCAAGGTATTAGTGAATCGCTTCACTCAGATGGTGATGCTCATGGTATGGACCTTACAATTAAATGTGCAGAAGAAAGACCAGAAAACACATTTGTATTCACAAACTTAGTTCAATTTGACTCGCACTATGGACACCGTAGAGATGTTGATGGTTATGCTTCAAACATTGCATTACTTGACTCAAAATTAGGTAAATTAATTAATGCTATGAAAGAAGACGACTTATTAATTATAACAAGTGACCACGGTAATGATCCTCTTTATCCAGGATTCAACCACACTCGTGAATTACTTCCTTTAACAATCTTTTCAAAAAGATTCAAAAAACCTAAAGTTCTTGAAGATGTTCTTGGTTTAGGAACAAGTGGAAATATTGTTGCCCGTAACTGAGGTGTTAAAACTATTGAAGAAACAGGTGACGATATCTTCGATCAATTAGTTTAATATTAAAAATAATACTTGCTTTTGGGCAAGTTTTTATTTTGCTTTTTTGCAATAAATAAAATCAATAATATACTAAAATATTTATATATGAACTTTACTAAATTCGTTAAAAATGAAATTATCAATCGCAAAAAAAATGAAGTTGAATCTCTTGAATACTTAAGAGGTTTAATTTTTGGAAATGGCTTAATTAAAAATGATTCCATTCATTTAAACATTCGTGATGAAGAATTTTTTTATTTAATAATTGAATTAATTAAAAAAGTAAACTTAGATTATCAAATTAAAAATTCTAAAACAATAGTTTTAAATAAAAAAGATATTGACTTAGATGTAAAATTTTTAAAGCCTTCTTTATTCTTTGGTGGAGTATTTCAAACAGGTGGAAGTATTAGCAATCTTGGTAAAACTTCATACCACTTGCAATTAAGCTCAAACTATGAAAATTTTATAGATATTATTATGAGCAAATTGAATGAATATTACTTTAATTTTCAAAAATTAAAACACCAAAATAAATACATAATTTATGTCAAACAAAAAGAAAAAATTGAAGATTTTTTGAAAGCAATTTTAGCTTTAGAATCATTTTATAAATTTACCGATATTACGATAAAAAGGGACTTTGAGAATAGTTTTAATCGAATAAATAACATAGATATTTATAATATTAAAAAGGCAGTGGAAGCTAATGTAAAACATATTGAAAACTTAAATTATATTTTTGAAAACAAACTTGAAAATAAATTCAAAGATGAGCAAATATTACTTTATAAAACAGTTCTAAATAATCCAGAAGCGCCTTTATCTAACATCATTTTATCACTTGAAAAAGAGCATAATTTAATTAAAAGCAAAAGCACAATTCATCATTGATTAACAAAAGCGAAAAGTGTAGTTGAAAAATACAAAAAAGGAAATAAATAATGAAAGAGCGAATGAAATATCTTAACATAAAAATTCAAGAAAAAATGAATCACATTCACTATTTAACTGGAACTGTTGTTCATAGTGTACAAAAACTTGAATATCGTTTAACATATTTAATCACTTTAAAAAAAACAATCGATGAATATGAAAAAAAAGGTAAATTTAAACATAATTTATTTTTAAAAGTTGTGCTAGAAGCATCTTCTGAAGCTCAAAAAATCTTTAGAAGTTTGTATGAAAATACTTTTGGTAATTTAAGCCAAAAAGCCTATCAAAATGGCATTTTGAGCAAAAATGAACATTTAGAATTATCTAAAGTTGTTGAAGATCGCAATGAATTAATTCATTTCTTTTTTAAGGATATTGAATTTAAACAAACCGATGATCAATTAGCTTTATATCTTGATGAAAGAATTAATTTTTTAGAAGATCTTTTTTCAAGAACTAGACGATGATATGAAATAATCAAAAACAAAATCAAGAAATTAGAAAAATAAGTTAAGAAATAATACTTATTTCATCTAATATTGCATATAATTAAAAAGTATGAATTTTATTTATGGCTCAGAAAATTTTTTTATTGAACAAGAAATCAATAAAATCGTTAAAAAATTTCCAAATTCTGCTATTACAACATTTAACATTGAAAACGATGAAAATGTTGATAGTTTAATTCAATTTATAAGCAGTGGAGATTTGTTTAATAAAAAAAGAATCATTGTAATTAATGATTTATTTTACTTTGAAAAAAAATTAAGCTCAAATGATGAAACTAATGTTAATAATTTAATTAAAAGTATTGACAATAATTCAAGTGATGAATTAATTTTTGTAAATACAAACATAAGCAATAAAGAGCAAATAAATAAAAACTTTTTTACTAATTTTATTTTCAACAAATTGCAGCAAAATAATTTTATTGAAGCTAATAGTATTGATGATAATCAATTAACTAAAAAAGTTGTTTCATTAGTACAACAAAAAGGCGGTACTATTGATGCTTTAGCAACCAGCGCTTTACTTAAAAAAATACCAAATGATCTTTATTTAATTAACTTAGAAATTGATAAATTAATTAATCAAAATAAACACATAACTGAAAATATGATTAATGTTTCAGTAATTGATATTTATGTAGAAGACGCTTTTGGTTTTAGCAATAGTTTTGAAACAAATGATTTCAATCTTATTTGGAAAAAATATAAAGAAAAATTAATTGAAGGAGTTGATGTTACTGTTTTAATTGCACAAACTTCACAATTATTTATTTTAGCTAACCAAATTTATGCATATAAAAATATAGGTAAAACTTTAGAAAATTTAGCAAGCGATTTTAAAATTAATCAATATAGAGTTAAAAAAGTCAGTTATTTATTAACAAGACTTGGCACTTTAAAAATTAGAAGAATGATTAAATCTCTTGCAAAACTTGACCAAGATATAAAAGAAGGAAAAACTGATCCAACAATTGGATTTGAAAGGTTCTTAATAAAATTCTTTATTAATGGATAATTTTTTGTACAATAGCACCTATTTAATTTATATTCAAATATAAAATAAAGTAAATGAAAACAAAAGAAAAATTATCAAAAAATAATATCTCAAGGAATTACATAAAAAATTAATTGAAAATAATTTTATTTCTAAATTATAGAGAGGAATTTGCATCAATCCACTAGAAGTTATTGACCAATATTATTTTTATGAACTCAAATATAAAGAACATTGTTTTTTTAGAAAATAGTGCAATGTATTTGCAAAACATAAATGATACTTTATATTACAAAATCGAGGCAAATTTTAAGCATGACTATAATACTTCAAAAGTAAATAAAGATATTAAGGCTCATCGCTTAGTTGAATGAAAACTTAATTTAGGAAAAGAAAAAGTTAAAACCATTTTTGGAAATTATGTAACAACATATAATAAAGAAAGAATTATTTGCGATTTAGTTGAAAGTATTGATGATTACGATCTTGAAACAAGACCGTAACTTTTAAAACATCTAAAATTTAAATCAAAATTATAAAAGATTATTTGATTATGGTAAAAAAATGGGTATTATAAAAAAATTATGAAAGTTTGAAAAAAATTAAAAAACAGTTTAAAAGAATATGGCAATCAATAAAGATAAGCGAAAATATTCAATTGTTGAATTTAAAAATGAGGGTTCTAAACTAAGGGACCTCATTTTTGCAACTTGAATTTGAAGTTTAAACAAAATTAACAAAAAAATGATAAAAATTGATATAATAAATCAAGCATTGGCTAACAGCTGCTTGAAAGAGTAGAGGAAAGTCCACGCTAGCACAACCTGCGATGGTTGTAGTGATCATGCTAGGCCCAATAAGCCTAGGCTTAGACGACTAGTGCCACAGAGACGAGAATTGTGAAACGCGGTAAACTCCATGAGTTAGAAACCCAAATATTGGTAGGGGAACTTTTCAACGGAAATTGAACCAAAGAAAAGAGTTGATTAATCAACTAGATAAATTGTTAGCACCTGAGAGGGTACAAAACGTGGCTTATAAATGCTAAAACACTTGCGCGAGCAGGCGTTTTTTATTTTATTTTTATACAACAACTGTGCCTAATTAAATATATAATTGAATAAAAATATTAATATGAGTGTAATTGATAAAATTAAAGAATTAATAAAAGAAAACAATGGTTATATGTCCACAGCAATTTTAGATAAAAACAAAATTTCAAGAAATTATTTGAAATTCTTAATTGAAAAAAATTAATTGTAAAAATTGATAGGGGAATATATGTAGATATTGATACTGCAGATGATGAACATTATGTATTTCAATTGAAATACAAAAAGTGCATTTTTTTTGAATTAGTGCAATGTTTTTGCAAAAAATTACCAATGTTTTATATCATAGAACAGAAGCAAATTTTAGTACAAATTACAATACATCAAAAATTGATAAGAAAATTAAATGCCATAGAATTATAGATTGAAAACTAAATTTAGGAGTTGAAAAAGTTAAAATAATGTTTGGCAATTATGCTAAAACATACAATAAAGAAAGAATAATTTGTGACTTAATTTTGGATATCAATAAATATGAACCAAAATTATGTCAAACGACTATAGAACTATATAAAGATTCAAAGCATGATTTGAAAAGACTTCTTAAATATGCAAAGATTTTTAATATAGTTGAAAGAATAAAATTGTTGTTTAATTTATAATATTGTATTTACTTGAATAACTATAAAACAGCTAAATTAGAAAAATTTTAAACTATCCAGTTTGTTAAATTATTATTAAAAAAGTAATTTTAATGTATCATTTAATAAGTATTTTAAAAATATAATTTTTAAATAAACAAAGGAGAATATTATGTCATTAAAAGCTGGTATTGTTGGATTACCAAATGTTGGAAAAAGTACACTTTTTAGTGCTTTAACTAAATATCAAGTTGAAGCTTCTAATTATGCTTTTACAACAATTGAACCAAATATTAGTAGTGTCCCGCTTAAAGATCCAAGATTATATGAATTAGCAAAGATAGTTAAACCTGGAAGAATTGTACCTGCAACTTTTGACTTCGTTGATATTGCAGGTTTAGTTAAAGGTGCAAGTAAAGGTGAAGGCTTAGGCAATAAATTTTTAGGTAATATTCGTGAAGTCGATGCTGTTGTGCATGTAGTTAGATGTTTTGATGATAAAAATATAATGCATGTGGCTAATGAAGTTAATCCAGTTAATGATAAAGATGTTATTAATATGGAATTAATGTTAGCTGATTTAGAAACCATTAATAATATTATTAATAGAATTCACAAAAAAGCTAAAAGTGGTGACAAGGAAGCTATTTTAGAAGAAAATTTAGCTTTAAAAATCAAAGATCTTTTAGAAAATAATAAACCAGCAAGAATGATTAAAAATTTAAGTGAAGATGAAGAAAAATTAATTAAAAATTATCATCTTTTAACTTCAAAACCAATGATTTATGTTGCTAATATGTCAGCTGAAGAAATAGCAGAGTATGAAAATGCTCCTTTATATAAACAATTAAAAGTTAGTGTTGAAGACGATGTAAAAATTTTGCCTATTTGTGTTCAACTCGAAAGCGAAATTAGCCAAATGGATGAAAGTGAAAGCAAAGAATGGTTAGATAGCTACAACATTAAATTTAGTGGTTTAGACCTTCTTGCTAGAGAAGCTTTTGATTTGTTAAAACTTAAAACTTATTTTACAGCTGGTGAAATGGAAGTTAAAGCTTGAGTGTTTCATGATGGAATGTTAGCTCCTGCTTGTGCTGGCATAATTCACTCAGATTTTGAAAAGAAATTTATTAAAGCAGATGTAATTAGTTATCAAGATTACATTGATAATAATGGCGAATTAGGCGCTCGTAATGCAGGAAAAATTAGATCAGAAGGTAAAAATTATGTAATGCAAGATGGCGATATTTGTCACTTTAAATTTGGCAAATAAGCCTATTTTATAGGGGTTTAGTATAATGGTAGTACCGCAGTCTCCAAAACTGCTCGCATGGGTTCGATTCCTATAACCCCTGCCATATCAAATTTTGATCAAGCAAGCTATAAGCTTGTTTTTTATTTAACTACTTGATTTTGAAAATTAAAAAATAATAATTTGAACAATTTAACTTAAATTTTAATATAATTGATTTATGCAAAGTAATAATTGACAAGCAAAAAATGGTGCTAATAATCAACAAGCACAAGATAATTTTAAAAACAAAGAAAAACCAAAGCCAAAAGAAAAAATTAAATTTAGAGGAACTAAATTTAATTTTAAAAAAGTTTTAATAAGTATTATTGTCTTCATTTTATTTTTTTTGATTAGTTTAAGTTCATTGATTACTTTCTATGTATTTAAAAATGAAACGATGCAAATAACATCACTTTTCTTATTATCTCTATTTGGTTTTATATTGTTAGTTTTTTGAATTATTCTTTTAATAGAATTATCAACAAAGAAATGATGATATTATTTCACTCTAACAATGTTTGGACCTTTTACCTTATTTATTATTTTAATAGTTGCAGCTATTTTATTAAAAATAGAAATTAAAGAAAACACAAGATTTGCAACAAGAGTTACAGGATATCGTTGAAAAAGAGATACAACTACTTTTAATGTAGAAGAAAAAATACAACAAAATAATTCACAAAAAAATTCTGAAGAAGAAGCAATAATTGTTACTAACTTCCTTGAAGATAACGAAGATTAAAAGCTGCACTTAATCAGTTTTTTTATCGCATTAAAAAACCTAGACAAATCAAGTTGTCTAGGTTTTTTAAGCATGAACTAAATTGATTTTATTAGCTTGTTTGCTTTAATGAAAATAATTATTTTCTTAATTTCATTTGCAATAGTTAAACCTAAAGCTAAAGGTAATGACATTCAAATTAAGATACCATTGCTTGCATAAATGCATTCATACATTCTAAATACTTTAGCTACTTGAGGCACTAATGCAACAAATAAAGTTGAGAAAGTTGAGAAGCTAGCAGCTAAAACAACTGGTCAGTATTTTTTAATATTGCTAATAAAAATACTTTTATCACTCATTAAATTAATACTATTTAATGAAGCTGCAACACACATTGTAATAAATGCTGTTGTTGAAGCGATTTTAACTAAATTAGCTGGATCATTTTTAATAGCTCACTCAGCTCCAAGTGCATAAGAAATAAGTGATAATAAACTTACTAAAGCACTTTGTCAAAGTAATTTAATTCCCATTCCTCGAGCAAAAATACTTTCGCTTTTTGAAAATGGTTTACGTTTCATAACATCTTTTCCACTATCAACAAAACCAAGTGCAATAGCTGGAAGTCCATGAGTTAATAAATTAATTCATAATAATTGGCTAGCTCCAAAAATGTAAAATCCATCTGGATAATAATCTTTATATGCTAAGAAGAAAGCGACCATACCAATAAGCATTACTAAAATTTCTGTTAGTGAGCTAATTAATAAGTTAAGTATCACTGTTTTGATTTTGTCAAAAATTAAACGACCATTTCTTACTGAATTAACAATAGTATTGAAGTTATCGTCAACTAAGATTAAATCAGCTGCTTGTTTTGAAACTTCAGTTCCTGTAATTCCCATAGCACAACCAATGTCACTAGTTTTAAGAGCGGGAGCATCATTAACACCATCACCAGTCATAGCAACTACTTTTTGGTGTGATTGTCATGCTTGAACAATTCTTAATTTGTCTTCAGGATTAACACGAGCATAAACTGAAATATGTTCAACTTTAGCTCTTAATTCTTCTTCACTCATTTTGGCTAAATCTTTGCCTTCAAGACAAGTATCGCTTTCTTGATAAATGCCTAAATTTTGAGCTATAGCTCTTGCTGTAGTTACTTGATCACCTGTGATCATAACAACTTTAATACCAGCACCTTGAGCTTCTCTAATTGAATTTGCAACCTCTGCCCGAGGTGGATCAATCATAGCAACAAGGCCAACAAATTCTAGATCATTTTCGTCCTTAAAGTCTATTTTACTGTGCTTTACGACTTTTTTAGCAACTGCTAAAACACGATAAGATTTGTCAGCTCATTGGTGATTAATTTCTTTAATATCATCAATAGTTAAATTATTACATCTTTTTAATAAAACATCAGGAGCACCTTTAGTAACTAAAAGCATTCTATCTTTTTCTTTGAATACTACAGAGTGCATTTTACGATTTGAATCGAAAGGCAAAGATGCTAAAATTGGATATTCTTTAAGTAATTCTTCTTTTTTATAACCATAAGAATAAGAGTATCTTAAAAGGCCTGTTTCAGTGGGATCACCTACTTCTTTTCATTCATTATTAGCTTCTAAAGTAATAAAAGCATCGTTACATAAAGCTAAATATTTAGCAAGCGGCCTAAATAATTCTTCTTTCTTGTTTGCACTTCAAAGAGTTCTAAAACGATAAGCATCAACTACTGTCATTTTATTTTCTGTTAGTGTTCCTGTTTTATCACTACAAATAATTGAAGCAGAACCTAGGGTTTCAACTGCTAAAAGGTTTTTAACCAACCCTTTTTGTTTGGACATCTGACTAACACCAATTGATAATAGAACAGTGGTAAAAGTAATAAGGCCTTCTGGAATAGCAGCAACTGCAAGTGAGATTCCAGTGACTAAAGCATTAGTATAAACTTGTGAATTTGTTCAATTTCCTGAGTGAACATTATTTAAAATAATTTGTAAAATAAAGCTTACGAATAAAAGGATAATTCCACCATAACCAAATCATTTGCTTAATTTATTAAGTTTGATTTGTAGTGGTGTTGCATTTTTATTTTGTTTTTGAATCATTGAATTAATTTTGCCAATTTCAGTTTCACTAGCTGTATGTTCAACTACAAAAGTTGCCCGTCCATTAATTACATAAGTGCCTGAGTAAACTAAGTGTTTGTTGTTAGCTAAGATTTTGTCATCAGTTGCATTTAAATTTGCGACTTTACTTACTGGTAAACTTTCACCAGTTAATGAAGCTTCTACAACTTCTAAGTTTGAAGCTTCAATTAAGCGACCATCAGCATTAATTGTATCTCCTGCAGCTAAAACAACTAAGTCACCAGGCACTAATAAATTAGCGGGAATAATTTGGACTTTGCCATCCCTAATTACTGTAGCATTGACCTGATTAACTTTTTCAAGGGCTCGCACCGCTTGATCACTTTTGACCTCTTGATAAGCTCCTAACATACTATTTAAAATAATAACTAGCATAATAATTCCAGGTTCAACATAAGAGATAATTAAATCGGTTGTGCTTTTTCCGCCTTGAAAATGTTCAACAATTGCCAAAGTTAAACTAATTGCTGCCGCTATGATTAAAAGAATAACCATTGGATCAACAAATTGTCTAAAGAAAGCAACTATTGGATTTAGTTTTTTCTTTTTAATTAAAGCGTTTTCACCATACTTTGCAAGGTTTGCTTCAACTTCTTTTGAAGTCAAGCCTTGATAATTGGAATAGTCTTTTTTGTTCATCTTTTCTCCTTAAATAAACAATGTTTTTATTATATATTAATTAATATAATATAGTTTAAAAAACTTGTTAGTTGCTATTAAAAAATATAAAAAACACTAATTCTTAAAATTAATGTTCTTCTTATCTTACTTTTTAAGTTTTTATTGTTTTCTCATTTCTTTGTGTATCTAGCTCTTCTATTTGCTTTTAATTCGTTAAATCTTATAATTATATCTCGAGTTCTCTCAACTATTTCATTTCAACGTCTTTCATTTTCTTTAGGATCTTCATTCAATTTTGGAAAGAAGCAACAATCTTCATCATGCTTTACTTCTTCTTTATTGTTTTCATTAACTTTGCCATTTTTGATTTTTTTTACATTTTACATATTAATACTTTCCTTTTAAATTATTCATAATAATTTTATTTAAATCTTAAAAAAATAAAAAAATGGCGGAACAGAGAGGATTCGAACCTCCGCGGGTGTTGCCACCCCTACAGTCTTAGCAGGACCGCCTCTTCGACCGACTTGAGTACTGTTCCACAAGTGCTTTTTATTATATAACAAAAGTTTAATAAATGAAAATAAAAAGCAGGCTAATTGCCTACTTGTCTTTAAAGAATGTCATTAGGATCTAAAATTAAATCAAAATATATAGGGGAGTGATCACTAATTGCATGAAAGATATATTTTAAATCTTTATCGTAAGTTTTTTTTCTAATAGTATCAACATATGTTTTTCATTCTTCTATTGTTTGATAATCTTTGAGAATATTATCTTTAAAAATATCGTATAAAGGAAAGAAGTTTGCGTTTTCATATTTTAAATTGGTTCTAGTAAAAATCTTGTCATAAGGTTCAGAATATTTTCCCCATGTACTATTACTCAATGAACTCTTGTGTTGTTTTGAGTCAGTTAACAGTGCTTTATAATATTGATCTTTTAGTATTTCACTAAAAGCAGTTGCTTGATTTCCTAGTTTTATGTTGGTATCGCCCACAAAAATAAGATCATCATTGTCACCATCTTGTTGATCAAATCAATCCATAAGGTTCTTTAAGTTGTAAGCTTCATCAGCTTCTTGAGCGCCTTGCTTACTTAATAAACTTGATTTTGATTCGCCACTTTTTGCTGCTCCTGGACTATCTAAATGATCTATAACAAAAGTGAAATTATTTTGAATTGGTCCTAAAGTTTTAAATTTAACTCCCATTGGAGGTCTAACATAACCAAGATTATTGTTTTCTTCATTAACAACAAAAGTGCTATTGTCATAACCTAATCATTCCTTGCCATTAATAAATTTTTCAATAGCAATCTTATCTTTTTTATAAATAAAGGCTGGACATTCATGTTGTTTTGAAGTTGGGGCTAATTTAGGATTGTGAATAGTATTATCAGAAGCTATTAATTCTCAATTGTTGTTTCCCGTAAATTTTTTTAAGTATTCACAAAATTTTTGTGCTGAAGCTTTTGTTTTATTTTCAATAAGCCCAATAACATCAAGATTTAAATGTTCAATAACACTAGCTAAACCATATTTTTTGTATTCTTCAGATGTTTCATATAAATTTAAAATATTCCACATTCCGATACGAATTTTAGATTTAGCTACTTTAAAACCAGTAATTTCAAATACCTGAATATCACTAAAAGTATTGTCTTTATTATTTATTTGAAAATGAACTTTGACTGAAGTTGGTAAGCACTCTAATTTGTTATAAGTAATGTTGTATTTTGTTGAGTCAATGTTTGCTACTTTTAGTTGAGTATAATCAATGACACTATTACTAAAAATGTTTTGTTTATTATTAACATCAAAAACAATTTTTTTAGCTATTTCATTTAATTCTTCAACTGTTTCGCCACTAATATTTTTGCTAACTTTAAAGCCTGTTAATATATATGTAAACTCATTACTTTCTAAGTTATAATTTTTATTTTTTAATGTAAAAGTTACTTTAATTTCATTGTTAGTTTTAGCTCAATTTTTAAAAATAACTTCTTCTGTGTTTTTATCAAAACCACTATAAAACAAGTCTTTTAGTAAAATTTCAGAGGTTAATTTTTCAGCTATTTTTTCAACTTCAACCTTAACATTTTTAACGGATTTATTAAGCTTATCAACAATTTCTTGTTTGTTAATTTTATTAGCTTTAAAACCTGTGAAAGTGATTTTAAAACTTTTACTTTTTAGATTATTTTTTTCATCTTTTAATACAAAATTAACAGTTATTGTACCTTCTTGATCATCTTTTTTTATGCTTTTAACTTCAACTAAATATGTTGCGCAATTGTAAAATTTTAATTCATTTTTAATCTCTTCATCATTTAAATTTTTAACATCAATTTGAGCTTTTTTACTTGCTTCAAGATTATCTTTATCTCATCAAATTTTGATTTTTTCAACCGCATTATTTAAGTCATTTTGAATTTCATCTTTGTTTTTTTCTTGTGTTTTATTCATTTCAAAAAAGCTACATTGAGTTGATAAAAAAGGTAAGGATATTAAAGAAAAAGACCCTACCAATAATTTAATAAATTGTTTTTTCTTCATAATATAAACACCACATTCTTATATATAAAATTATATTAAAAAAATTAAAATTGTTTAAATTTTTATTTAGTTGACTATTTTCATATTATTTCCAAAAGTGGGGGTGGTAAGTTTAAAGAAAAATGCATTTTCTTAAATTGACTGCTTAAATTTGATTTATTCTAAAAGTCATCATTATGAATTTTGTGTTTGAATCATCTTTTGTCATAATGTTATTTGTCTCAATATTTTCTTCTTTTTATTTGTATAAAAAGCTGAAAAAATAGAATTTGTAGAACAACTATTCCACCAATTAATAAAGATATTTTTTTCATATTTTTTATTTTGACATTTCTTTTTTGCAAATACATGATTATTTGAATAACTAGTATATAAAATATTTTTTGCTTTATTCAAATGCGAAATAAACAAAAAATAAAAAAGATAAACAACTAAAGAAAATTGTTTTTTAAGAATTATTTAATTCTTTTTTTCTTTTATTGTTTAAACTTTTTATAATTTATTAATTTGTGAAAAAATTAAAATTCTTTAACTTTTTCATTAATAAAACTTTTAATCTTGTCTGCAATTTCAGGTTCTTCTAAAGCATAATCAATGTTTGCTTTAACAAAGCCTTCCATTGATCCAAGATCATATCTGATGCCTTCAAATTCATAGGCATAAATTTTTTGTTTATATTTTTTCATTAATTCATCAAATGCATCAACTGCTTGAATTTCATTTTTGCCATCATATTCAATTCTAGATAAGATATCTAAAATTTCTGGATTAAAGACATAACGTCCAAGTATAGCTTTATTTGATGGTGCTTTATCAGCCTTAGGTTTTTCAACTGCACCATTAATTTCAAAATATTTCTTATCTTTATCTTTCAAGTTTATTGGATTAACAATTCCATATTTATGAATATATTCTTTTGCTACTGATTGTACACCAATAACATTTGCTCCAGTTTTATTATAAAACTCGATTAATTGCTTAATAGCAGGAGTTTTTGATTTAATTAAGTCATCACCTAAAATAATAGCAAACGGCTCATTGTCAATATATTCTTTACAACTTGCAAGTGCATGACCTAAACCAAATTGTTCCTTTTGAATTACTCTAGTAATACATGAAGAGCGATTTGTTCTTTTAACTTTTTCTAAAATATCTTTTTTACCTTTGATAATTAATTCTGTTTGTAAATCTTGATCAACTGAGAAAAATTTTAATAAATCCTTTTTCCTTTCACTTAAAACAATTAAAATTTCCTCAATACCTGAATCAAGTGCTTCATTAACTAATCTATCAATAACAGGTGTATCAAGAATAGGCACTAATTCTTTATGAATAATTTTTGTCATAGGTAAAAATCTTGTTCCTCAGCCAGCTGCAGGAATAATCAATTTTTTAATTTTTTTAATTTCATTCTCTTTTGTTGTCATGTTCCTAATTATACTATATTGTTTTTATTTTGGAATGCTTCTCTAGCAATGTAAAAATTTGAAAAAAATAAAAAATCAGTCTAATGACTGACTAAATATTTTGGTGCTTAAACATATGGCGATCTTAAAGGGACTCGAACCCTTAACCTTTGGGGCCGAAACCCAACACTCTGTCCATTGAGCTATAAGATCAAGTTAATTAACTAACTCTATTATAAGTGATTATTTAATATATGTCTCAATTTAATTTTTTCTTTAAAAATTTTACTATCTCGCAGTCTACAGCTTCTATTGCATTAATTGTACTGAGTTGCCTAGTTGCTTCGTTACAACTAATTTTGCAACTAAAAATATAAAATCATCTGAAAAACTTTGTAATCTTTTGGCTTCATTATTAACTAAATAGTGTCTCAGTTTCTTCTATTATTTAATCATGAACCTTTACCACCTACTCTTAATGTATAACCTATTTCTTTATTTACTTCTGCATTGTCAAAAACTCAATTAAGTTTTTTGGTTAATGGTATTGGTTTTGGAAAAGTGAAATTATATTTAAAATCTTTTCTTATTCCAAGCATAAATAATCTTGGTCTATGCTGTTGCAAATTAAAATCAAAAGCTTTAACTATTTAAAAATGAAAATTGTAATCTAATTCATTCAATGTATTTTGGATAATTTAAAAACTTTTACCATTATTATAATTTTTTAATTCTCTAACATTTTCTAGAAAAATAATTTTAGGTTGCTTTACTTTTATTATTTCAGCTATATTAAAAAATAAAGTACATCTTGTATCTCCAAAACCTTTTTTATAGCCTATTTAGGAAAAAGGCTAACATGTAAAACTTGCACAAAGTACATTATGATCAGATATATTTTTGGATTTATTAAAGTTATATCACCTGCAAAAAAATTAGTTGGTTCATTATTTTCATTGAATAAATTTTTGCTATTTTTAAAATTTGTCGCATATGCTTTTCTTGCATTTTTATCTCATTCTGATGCGAAAACGCGTTGACCACCTAAATTGTTCATAGCAATATGAAATCCACCTATTCCATCAAATATGTTAATAAATTTAAAGTTTAATTGTTCCTTTTTATTAATCGTTATTAATAATTTAACATTATTTTTTTATAAATAAAATCAGTTTTATCATAAAAAGGAATAAAAAAACTTCGTCAAGACGAAGTACATTAAATAATGATATGGTGGCTCCGGCAGGAATCGAACCAGCGACACACGGAGCTTCAGTCCGTTGCTCTACCAACTGAGCTACAGAGCCATAATGGCGGTCCAGACGGGAATTGAACCCGCGATCTTCTCCGTGACAGGGAGACGTATTAAACCACTTTACCACTGGACCAATTGGTGGCGGAGGCTGGACTCGAACCAGCGGCCTCGAGGTTATGAGCCTCGCGAGATACCAAACTTCTCCACTCCGCGATGTGATATTTTTTTATTTAGGTGTTCATAAAGAACAAATGGCGGGTGATGAGGGATTCGAACCCCCGCGGGCCGTGAAGCCCCTGCTAGTTTTCAAGACTAGTCCCTTCAGCCAGGCTTGGGTAATCACCCTTGGTGGACCCAACTGGATTCGAACCAGTAACCAACCGGTTATGAGCCGGTTGCTCTGCCGTTGAGCTATAGGTCCAAAGCAGTTAGGATTTTCTCCATATAATGGTAGCGTCGATGAGAGTCGAACTCATGACCTTCCGGGTATGAACCGGATGCTCTAACCAACTGAGCTACAACGCCATAAATGGTGGAGGATATGGGATTCGAACCCACCACCCCTTGCGTGCAAGGCAAGTGCTCTAGCCAAATGAGCTAATCCCCCACATGGTGAAGAAGACAGGATTTGAACCTGCGACCACTACGTCCCAAACGTAGCGCTCTGCCAAGCTGAGCTACTTCTCCTACTTTCAATGCTTCACTTTCTAAATCAATAAGATAATAAAAAGCATATATATTATATACTAATTTGTCTTTTTGTAATAGAATAATTTATTTTTTTTATAATAATTTTTTTTAACTTATAAAGCTTATTTATAGTGTTTTTAGTCAAATTGTTAATTGTTTTCAAAAAAGAATTTTATTTTTATTGCTTAAGAAATTAAAATAGTCATTTAAATAAATTGCTTATTTTTCACAAAAATTCCATTAAGCTTTTGAATATCTAAATGTTTGCTAAAATATATATAATAATTTGATAAGGATGAACAATGAAATTAAAGATAAAATCTGATTTATTAAACAAAATTAAAAACATTCATCGAGAAGAATTTTTATATTTAAATTCAAAAATACTTGATTCATATGCTAAGGGAAAATTAATAAGTTTTTACGATGAAACAGATAAAAACAATTTCTTTATTTTTGAAATCGTTGAAATACAAAAAGTTAATTTTGCAGATATTAAAGAACCTTATATTATTAATACAAGTAAAAGAGATCATTGATATAAATTGAGGATAAAAAAGGATTGATTTGATCTTTCAATGGTTGATAACTTTGTATTTGATATGGATGGTACTCTTTTAAATGAAAATAAAGCTATTTTAAAAGAAAATATTGACGCATTAAAAGAACTTAAGAAAATGGGCAAAAATATTATTGTAGCAACTGGACGTCCATTGTATACATTGCATCATATTGAAGAAGTTCCAACTGAGTTTCCAATTATTTGTTCTAATGGAGCAATTATATATAACAAAGACCATAGTTTATTAAAAGCATATGAAATAGATAAGGAAACAGTTAAAAGTATTTACAAATATTTAAAAAGTCAAGATATGGAATTCCTAATGTATTCGCCTAATTATGTTTTAGGTTGTGAAAATAGTAATTGCAAATATCTTGAAATGCGTAATTATGCTGCAAGTGTTGGTGAAAATTATAAACAAGGTGAGTTCTTTAATGAATTAGAAAAATATAGAATTTGTAAGTTCTTAATACATATACCTTGTACAACAGAAGAAAAGTTAGCAAATTTAAAAGAATATTTAAAACAATTTGATGTTTATTTAGTAAGATCACAATCGACTCTTGCTGATATTATGAGAACAGATGCAACTAAAGGTGAAGCAGTTAAAAATGTTGCTCAAAAATATAATCTTGATTTAAATCGAACAATTTGTTTTGGCGATGCAGAAAATGATACAAGTATGTTCTCAATCTCTAAATACTCAGCTAGTCCTTCAAGTGGAAATATTAAAGCTAAAAATAGATCTTTATTACGTGTTAGAAATCATCAAGTGGCTTGATTAAAAGAATTTGTAGAGCAATTCAAAGACTAATTAAAATAGTATTAAAACTATTTTAATTTTTTTACTTTTTTAATTGTTTTATAGGCTTTTTGGTAAAAAAATGAAATAAAAAATGTCACACTTGTGACACATGTTCTCTGAAAACTGAATAGTAACAATAATTCATTGAAATGTCATAAATACACTTCTACTTTTAAACCGATCGATTTATTAGTATTGGTCAGCTCAACGTATTACTACGCTTACACCTCCAACCTATCAACCTCATAGTCTATAAGGAATCTCAAGGGAATACTTATCTCTGAGGAGGCTTCCCACTTAGATGCTTTCAGCGGTTATCCTTTCCGTACTTAGCTACCCAGCTATGCTCCTGGCGGAACAACTGGAACACCAGTGGTACGTCCACTCCGGTCCTCTCGTACTAAGAGCGGCTCTCATCAATATTCCAACGCCCACATCAGATAGGGACCAAACTGTCTCACGACGTTTTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGAACCGACTCCAGCTCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTTGCCGTCGATGTGATCTCTTGGGCAAGATAAGCCTGTTATCCCCAGGGTAACTTTTATCCGTTGAGCGACTGCCATTCCACAATGTACAGCCGGATCACTAAGTCCTGCTTTCGCACCTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGAGCCCAAATTAACGCCGACTANCAACACTACAGACTCTTACGCCAATATTCCGATAACGCTTCAACCTATGTATACCGCGGCTGCTGCACATAGTTAGCCGTTGCTTCTAATAAGGTACCGTCAAAACAAAATCATTTCCTATTCTGTCTTTTCTTCCCTTACCACAGAAGTTTACAATCCATAGGACCTTCATCCTTCACGCTGTGTCGCTCCATCAGGCTTTCGCCCATTGTGGAATATTCCCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGTATCTCAGTCCCAGTGTGGCGGTTCAGTCTCTCAACCCCGCTAAACATCATCGCCTTGGTGGGCCGTTACCTCACCAACTAGCTAATGTTACGCACCCCGCTCCTCCAGCGAAGCAAACGCTTCTTTTCCGTAATCTTCATGCGAAAACTACGAGTATTTGAAATTATCGACTGTTTCCAGTCGCTATGCCAAACTGAAGGGTACGTTGAGCACGTGTTACTCACCCATTCGCCGCTAAGGTATTGCTACCTTCGCTCGACATGCATGTATTAGGCACACAGCCAGCGTTCATCCTGAGCCAGGATCAAACTCTCGAAAAAATTGACTGTCATGTTTATATATCTAGTTTTCAAAGAACAATTTTGTCGCATTATTTTGTAAATGCTTTAATATGATAACACATAAATTAATATTTGCAAAACATTTTTTTGAAAATTTTTTTATTTTTTTACAAAGTATTTTAGAAATTATTTTTTTATGCTAATTTAACTTTTTTAAATTATGAAAAAATAAAAAAAGTGCCTTATTTTTAAAGCACATCTCTTATATATAAATAAAGAAAGGAATTTTTTAGAATTTGATTATTTCAATATTTTTTTCTAAGCATTCTTTTTCTAAGTCATTAATCATGTTTAATTCTTCTTCTAATTTAAGAGCTTCTTGAACATTAGGTTTAGTTACTGGTTCTTTAGGAGCAAAAAGCTCACATGTTTCATTACATTTAGTAATTGAAAGATCATAGGTTTGAATTTTTTTAGCTAAATTGATTGTTTCAATTTTGTCATTAGTTAATAGTGGCCGCAAAATTGGCATGTTAATATCATGACTAATTGTATAAAGTGATTCTAAAGTTTGTGAAGCTACTTGACCTAAGTTTTCGCCATTAGAAATGGCTAGATTTTTTTTACTTTGAGCTAATTTAGCAGCAATACGATAAAAGCTCCGACGCATAAGAGTAATTTTATAAGCTTGATTTGAAACTAAAGCTATGTAGTTCATTAAAGTAGAATAGTTAGCTAAATAAAAAGTGCTTGTGTTTTGATATTTAGTTAATATTTTGATTAATTCTTTCATTTTATTTACTGTTGCTTGATCAGTTTGAGGAGGTGTAATAAAAGATAAGAAATTAACTTTAAGACCTCTTTTCATAAGTTGAAAAGCAGCAACAGGACTATCAATTCCACCACTCATTAAGTGCAATACTTGCCCACTAACACCAACAGGGAGGCCGCCTAAAGCTTGGACAAAATCACTAAAAACATAAGTAAATTTGCCTCTAACTTCAATATAAACAATAAAGTCTGGATTATTAACATCAACTTTTAAATTTTGGTTGTTTTCTAATAAAAAACCGCCAACTATATTGTTAATTTGACTTGATTGGTATTGAAAACTTTTATTGTTTCTACGAGCAGCAACTTTAAAAGTTCCATTTTTATTTTTTGCTAAATTTAAAGCTTGCTTTTTAATTTCTTCAATATCATTAGCACAAATAATAACAGGTGAGAAAGAAGTAATACCAAAAATGTATTGCAATGCATCTAAATTTTCTTGACTATAAGGCAAAAACATACGGTCAAATTCAACCTCTAATTTATCTTTAGTTTTATGACGAATATTTGAAGCTAAAGTATCAATAAAATTAACTCTATTTTTGCCTTTTAGAGTCAATTCACCATATCTAATTAAAATCTTATTGTACATATTTTTTTCCTTTTTTCATGCAGGTTGCTAGTAGTGAGAAAGCTTCATCAAATTTCTTAGCTCGCATGTGTTTATTGACTGAAATTAAGAGTTCATTAAAGTCTGGATTATTAAATCGATAATGCATGCTTTTTAACATGAGCTCTTCTGTCATTTTCTTATATAAATAAGCCTTAAACGTCATTTTGAATAGCTTTGTAAAGATGTTGTAAAAGTGGTTTCAATTTTTAGATTTGAAGTTAATTTTTTTACTTTGTTCAAAGTCTTTTTTGACTTTTAATAAAGAAACAATTAAAAGCTTAATATCGTTTTCTAATTCTAAACTTTGTTCTAATACTGTGCTTTGAACAAATTGAATTTTAAGGCTTCAAGTTTCCAAAATTTTTAAGTAGTAAGCATTGTAATTTTTTTGATAAGTATCGAGGTTAAAAATCTCAATTTTATCATTGCATTTTTTGATTAATTCCATTATGCCATGAAGTAGTGTTTGCATCTCCATTAAACTAACATTGCCATTAGCTTTATTAGCTTTATTAAAGCTATCATATTGGTTTCTAAGTTGTAACAATTCTTGTGAAATCTTGGTTACAAAATTGCTGTCAAAATCATCTAAATCAATTGAAATATAGCTTAATTTTTGATCAAGCTCATTAATCATTTTGCCTATGTTTTCTAATTCATTATTTATGAAATCTTGAAACTCAGCATTAAGAGCAGTTTTCTTGTTTAAAGTATAATAATCAGCATAGATTTTTTTGATTCTTTCTTGCACATCTTGATAAGGTAATTTTAATCATTCAGTTGCTAAGTTTTCTCTTTCGTCAATTAATTGTTTGATAGCTAATTTGTCTTTTGTTTCAAAAGAGAGCTTGCTTAAGATTTCAGGTAAGTGGTTAAAAAGACTTCATTCGATATTAACAAAGTGGTCCACTTTGCGAGCAAAAATATTGATTCTTTTTTCATGTTGATTTAAAGCATTTGAAATACTATCAATTTTATGAATAAGTTTATTTTCTTCTCATTCAATTGTAGTGTTACTTAATTGTTTTAATTCATTAGTTAAATAATCATAACTATGATTTAAGCAATATTTATTTTTTTCTAAATGTTCTTCAATTTTTCTTAAGATTCTAAATGATTCATTGGTTACTATTTCAATAACATTTCAATGTTTATTTAAGTTCTTAGTTTTTTCATCAAATTGTTTATGCATTGAAATGCATTCTTCATATTTAGGCAAAATTTCTAAGTAAAGTTTTTTAGCTTCTTTAAGTTTATAAGCTTTAGCCGCTTTTCTTAATGCATTATATTTATCATTTAAATCAACAATTATTTTTTTTAATTTGTTGTCTAAACTTTTTAATTGATTTAAAGCAGCTTTATATGTTTCTTGAGTATTTGATAAAGTAATAAAACGTTGAATTGTTGCGTTACGTCCTGTTAGATTATTTTTAATATTTTTAGTTGAATCATCTAAAAATTTAATGTATTTATTAAGATTAGTTTTTCTAATATAAAGGAAAATAAAAATGCCCAAAAGACCTAAACAGGCCAAAATAACTATTGGTACAATAATTTTAATTACTTGCATTTTTCTCCTTAATTAATAATTATATTTTATATTAATATCATTTTTATTTATCAAAGTAGCATTAAAGAATAATAAAAAAATGGGATTGCCCATTTAATTATTATTAAGCATTACGCATATCTAAACTTTTGTAACCTTCAACTTTTTTACCTTTATAAAATCCACAAGATTCACATACACGGTGTTGTTGAATTAATTGAGAACAGTTTTTGCATGCAACAAGATTTTGAACAGGTAAAGCATCATGGGTACGTCTTTTGTGTTTACGTTGTTTAGATGTCTTACGTTTTGGAACTATAGCCACGATTCCTCCTTATACAATTTTTATATTAGCTAATAAATGTATTCTAATTATATGTTAAAAAATTAAAATGTATAGCAATTTATTAAAAATTTTTTTTGTAGTGTAACTAACATTTTATTGCTTTTTAAAGCAATTATTTTCTTGGCCTATTTTACAGTACTTTTAGTTAAAAGTAATATCTTGAAAATAAGTAAAACCATCTAGATTTATTGGTTTTGTGTAGAAGTATTGAACTATTGATTTTTGGTATTCGTTAGTAAAAAGGAAATTATCTTCAGTTACTCTGATTCCTAAAAATGAGTCAATACTTTTTAAAATATTGAATTGTTGATCAAAATTTAAAGCTTGAATTTTTTCTTTAAATCTAGCTTTGAATTCTTTCAATTTCAAATTATTTTTTTGCAAAATTTGATCTAAAAGTGAACTGTTTGCTTTAATTTTTTGCAACTCATAATTTTTAAAGTAATCAACTTTATTAATTGTTTCTTCTATTGTTGAAATAATTTTTTTGATATTTTTATCATAGTTAATTGATTCTAAATTGACTTTTGAATATAAGAATAAATTTAAAAAGAATTGGTTATTTGCTGTTATTAAATTAATTAAATAGTTACTAAATGAATTATCAACTGCACTAAAGTTTTGATATTTAGAAAAGTAATTATTTGCTTCTTTTGAAGCATATTCAAAATTAACATTTAAACGATTGTCTAGGTTGTTGTACAAGAAGGCTATTCTTTTATATAAACTGTCAATAAATGAACTCTTTTCAGCAAAAATAGGTAATGTAAATTGAATTTTTTGATTTTTATAATCTCCATTTTTGCTATAAAAATCATCTAAAATTTGCTTCATTTCATTTTGATAAATTTGATAATTTGCACTCTTTAGTTGTTCATTAATATCCAAAATATTTTTGCTATTTATTGAATGTTTAATTAAGCCATCAGATCCATTAAAATCAATTATTTTATTATCAAATTTATTAGTTTTATAATCAAAATGATAATCATAAACTCGATTAATGTCATCTAAAAAGTTAACATTTTGGTTGTCTAAAATACCACTATTATTGCTGCTGTCATAGTTTAAACTTTGTGAAGCAAATGAATTTCAGTAAGTATTATGATTCAAATTTTTAATAAATGAATATTGATTAATACAGTTATTTAAAATATTTCTGAACACAATTTGATGTGGTTCATAATATTTTTTATTTACTAAATTTTGATCTTTTAATTCATTTAATTTCTGATTATAAAATAGTAAGCTAAAAGCTTCATTAAATTTGAAATCTTTGTTATTTTTAGCATATGGATTAACATTGTAAAAATAGCTTATAACATTACTATTATTTGGTTTAAATAATGTATAAGTAACACCAAAAAGATTAGAGAAATTATCTATTTCATCTTTTTGCAATTGGTTAAAAATATCATAAGAAGCTTCACTTAAAAGATTTTGTTTAAATGCATTATAACTTTGTAATCTAAAGGTAGGCTCATCGTTTTGAATTGGATTATATTTTATAGTCACTTTATTAAGAATATTTTGCTTTTCATTTTGGTTAAAACTATCTAAAGCAGAGTTATTATTTTTAATAAAAATTTGCTCATCAACAGTGTTCTTATTTAAAACATAATAACCTATAAAACAGATGTTTTGGTAATTTTTGCCATAACTATTTTTATTATTTTTATAGTAGTCTGTTGATATTGGATTGAAGATCAAATTATTAGTAATTTCTTGAGTTGTAAATAGTTTAATAAAGTTATTATTTTTGCTTTTTTGAGCATCGTTTAATTCAAAAATTAACTTGTTATTTTGGATGCTATTTTGGCCTAAATTTTTCAAAGAATAGTTGTCTAAAAGAGCTGAAAATTGTTCACGATTTTGTTTCAAATTAAAGCTAGTTAAATAGTCTTGGGCTTGTAATTTATGATTTGTTTTTTTGCCTTTATAATCTGAATAATAAACATCGTCTTTTAATTCAAATTCAATTTTAGTTGCACTATTTAAAGCTTCAAAAAATTCTTTAGAATTTATATTATTATTTTCATCAGAATAAATTTGTTTAATACCGCTTAATGCTCCATCTTCATTAACTTTAGTACTACTTTGAAGAGCTTGATCAACTTTTTCTTTAACATCATCACTATTAAAAATTTTTTGACTATTATCCTTCAGAGTCACTGTTATTGATTTAGCTAAATAGAATTCTAAAAATCTAGTTGTAGTTTTATAAAAGAATTTGTTTAAGTGATCAAATTTACTTTTGCCTAAACTTTTTCATCTAATTAAAGGTGCAGTTAATAAACTCTCAAAATCATTATCAATTCTTTTAACTTTGTTAATAATAAAATTGTCTTTAATTTTGTTTGGTTCATTATATTTTTTAATATAACTATCTTTAGGATTAACTTGTCTAATATTGTTGCATGAAAGAGTAGCGACACTAGAAGTAAGTGTTGCACTAATTAAAAATAAGCTGCCATATTTTTTAATTTTTCTTTTCATACTATCCTTTACTTTTCTAATTTAGGTACACTCGCAATAAAGAAATTTATACCTAAGAATTTTACTAATATTACATAAAAACTTGCTAATATTGAAACAAAAATTGTATATTCAACATTTTTTAGATTGTCAACTATATCTTTAAAAACGTTTCCAATATTTAAGCTTTGATCAATTTTATAAATATTAAAGAAAGCTAATGAAGCTAAAGCTAACATATTAAGTGATAATGAATCAGAAACTAAAGCAATATTTCTTCACAAGCAGTGAATGAAAATAATTTTGTAAATTATTCTTCAATTACTCAAGCCATTAGCTTTGTAAGCTAAAATAAATTCACTGTTTTTGATTTCAAGCCCTATTGAATAACTTGAATAAAAGAAGCTAAGGGAACCAATAAAACCTAAAATTAATATAGCATTAAGATGGTTATATGAACTTATTTTAAAAATCAAGAAAACTACAATAAGGAAAGGCACAATATTAATAGTACTAATTAAATAGTAACTAAATTTAGCACTGCTTTTATTTGAATAATAAGCCACTATTGAACCTAAAAAGGTCCCTAAAAATAATTGGATTAAAGCAGCCACAAATGTTACTAAAATACTTATGCCAAATGAATGAATAAAGAAACTAAAGCGGTCTATACCTTGAGAATTGGTACCATATAAAAAGATATAGTTAGTGCCTTTTAAGGCATAAAGCAATTGATATGGATTGTAAGTTAAATTAACTAAGTCAAAATCAGGTGTTTCATTTATGACTATTTTTAAATTAGGATGATCTTTTTGAGTTTGCAAAATAAATTGATATAAGGGGTCACTTTGTGCAAACTTTTTAGTAATACTTGCATTTAAGTAGTTAGGCAAATTATAAGCAAGTTGTGAATCGATGACACTAGTTTTTGGCGAATAAGGGCTAAAAATAGCACTTAAAGTCAAAAACAGTAAGAAGATTCCAATAATTGAAACTAAAGCAATGTTGATCTTTTTGCTAAAGAAACGAATTAAGTATTGTTTAAATTCACTTTTGTTATTGCTGATATTTACTTGGAAGTGTTTATGGTGAGTTAAAGTGAAAAGACTTTTTTCAAATTCAATATGACTTTGGCCTGCTTTTTCGTGAGAATGGTGGTGCTCATGATTAAATGCCATTATGCGCCTCCTTTCTGAATCTATCTTTTTCTCTATTTTCTTTGAAGTTAAAATTGAAATTAAAATTATAACCATTTTCAACTTGCAATAAATCATAAGTTAATTCACAAATAAAAGCTAATAAGAATAAAAGCAAAGCTTTAAAAAATACTAAAGCCATAATAACATTAATTTCTTTTTGATCAAACATGCTAATTAAAATAACACTTTGACCTGGTATTTGAAAAATACGTTCGATAATTAAGCTAAAACTTATAGTTAAAACTAAAATTGGCACTATTTGGTTAACTAATGAAATAAATAAATTTTTAATAACAGCTTTATTAAATATTTGTAAAGTACTTAAGCCATTTGCTTTTAAAGTGATTACATAATCTTTTTTTAGAATTTGAACAAGATTATTTTTGGTAATGGTTGCAAAGAATGCAATGATTCCTAAAGATAAAAGTGAAATAGGAAGTATTAAAGAAAGCAATGTATAACCAAAACCAAAAGCTGATGGCTCTATAAAATTAACTGGTAAGTCATTGACTTCTGAAATAATTAGCATTATTGGTGCAAAGATAAAAGTAGGAATAGCAGCTAAAATAAAGACAATAAATGAGAGGACTAAATCAGTCATTTTATTGTTTTTATAGCCTAATCATATTCCGACAAAAACACCTATAAAAAAGCTAAATACAAAAGAAATGCTTACAAAAAGTAAACTAAATTTATAATAATGGAAGAATAATTCTAACGATGAATTGAAACCACTATTATTAGTTGAAAATGGTTTACCAAAACCACTAAAAATAGAACCTAAGAAAGTGAAATAATTTTTGATAATTGGTTCTCTGTTTATTTGTGAGTTATTAATAAATAAAGCTATAACAAAATAGCCAAGGGTAACTGTTATAAAAATACCTAAGAATGTAAGTAGAATTTTTTTGAAAAAGTATTTGCTAAAATTCATAAAAATTCATCTTTCTACATAAAAAAACCGCTTAATGGCGGTTTCTTATTACATTAAATAATTTCATTTGATTTAAAAGCATCATAAATAGCTTTGTATGCTTCTTCTTCATCTGCTCCATTGATTTTAATAGTAATTACGTCGCCTTTTTTAACACCTAAAGACATAACATTCATAATTGATTTAAGATTTCCAACTTTTCCTGTCTTGTCTAAAACAATTTGCACATCACTCTTGAATTTTGAAGCAATTGTTGAAACAACACTAGCAGGTCTTGCGTGAAGACCAATTGGATCTATAATTGAAGCTGTAAATTCTTTCATGTTTATCTCCTTATTAATTTAATATTTCAAAATATATCTTCTTAAATATCTAAAGTTGCCAATAAATATTATTAATATTATATACTAATTCTTATATATACTTATAATATTACTACAAATGTCATTTTTAAAATATAAATTTTAAAGAATTTTTTAGTGGTGTTTTTAACTTATAAACCTTTAAAACAGTTTGTTTGGTAAATTATTCTGCTTTTTCAACTTTGAAAAACTTTTCGTTTAAGGTTTGAATATTTTTTCCAATGTTTTCAAAATCGATATTACTACTAATTAATGCTTTTGTTATGTTTGTGATTTTATTGAAAGGTAAATCAACAGCAATACCCGTTTCTCGACATTGATATTCTTTGTTTGATGAAACTAGTTTTGCTTTTTCTAAGGCTTTAATAGTTTCATTAATTTGTTCAGTTTTAATAAATACTTCAAAGTTTGAATTTTTTAAAATTTCTAAGTCACCAGAATATAAAACTTTACCTTTATCTAAAACAGTTAGACTATCAATATATTGTTCTAGCTCACTTAAAATATGAGAAGAAACAAAAATGGTTTTGCCTTGTTTGTGAAGTTTGCTTAAGTTAAAATAAAGTTCATTTCTAACTTTTGGATCTAAGTTTGCTGCAGGCTCATCTAAGATCAATATTTCTGGATTATGAATTAAAGCTTGGACTAAAATAACTTTCTTTTGTTGACCTGAAGATAAATTTTTACCTATCTTTTTTCATATTTTTTTGTTAACATCTAACTCGATTAATAATTCTTCAATTCTTTTTTTAGCTTCGTCATTTTTAAGTCCATTTAATTCAGCTAAAGCAACTAAGTAATCATAAATATTCAGACTTTTAGGAAATGTTGCTACTTCAGGAACATAACCAATTTTGATTCTACTTTTTTTGTTTTTAAAGTTAATTCCATCTATTAAAATTTCACCTTGGACTTCTGGATAAAAATACAATAATGAACGGAAACTTGTAGTTTTACCTGCTCCATTTGCTCCAATAAATCCATGAAATTCACCTTTTTTGATTGAAAATGTCACATTGTCTAAAGCTTTAAAATTTTTAAAACTCATTGTAACATTTTTAAATTCAATTGCGTTTTGTGACATTTTTTCCTCCTATTTTAAAAAGTTAGTTTTTTTGTAAATAAATCATCCGCCAATAGCTAAAGGTGTAGCCATTAAAAAGTAAATAATTATTTGCATGTAAGATGGAAATATATTAACTTTTTGTACTTTGTAAAATTTCAAATCAATGTTGTTTTCTTTTAATAAATCTTTATTAATTAGCATGCTGCCAAAAATTTCTTCAAGTCTTGAATTAAAGAATTCTTTATTTTGATTTATTTTTAAAGTTTCGCGATAATCATTCATAATAACTTTTGACATTGCATCAACTATAGAATATAAATTATTTTTTACCAAGATTGTAAAGAAAAGTTTTTTTGCAATAGAGTTATAGTCAAAGCTTAAGTAACGAGTTTTTTTAAATAATTCTTCTTTTTTGTCAAAAGTTAAATTATCTTCAACAAAATTTCTGAATTCTCTTTCTTCATTTTTTGCTTTTGAAGGATCTTCATTAGCTGAGAGAAAAATGGTACTTCTATATATTAATGGCACCATTTCATTGTAAAGTCTTTGAGTTGCAAAATTAATTAATGTTTCATATGTTTCAAGAGTTTTGGTTGAGTCTTTACCATCAAAATTTGCTTTAGCATTGACAATTTCAGTTTTGAAATCGGTTTTTAAATTTTCAATTACATTAACAAGACCATTAAATAATTTTTGACTAAAAACATCTGGATTTGCTTTTAAAATTTGGGCTTCTTTTTCAAAACGATAAACTCTATCAGTATATTCATCATTTCATTTTATAATGTGTTTTTTGCGAGCCATATAAGTAATTTTTTGTTCTAAGGTTTCTTGTTTTTGATATGGATTAAGTCGATCTGTTGTGCTTAAATCATAATCAATAATATAAATCTTTTGATTTCCATTTGTGACATAATCATAATTATTCAAAACAGATTCCATATCTCTTTTATAGAAATATTGGTTATGAACTCCTTCACTCATAATTGGAATAAAGCGGTTGCTCTTTGGATCATCTAAAATAAAATCATTATAAAAGTTCATTGATCAAAAGTTCATATTTAAACCATTAGATAAATTGACAACTGAAAAATTATGTTTAAATAAATTTGAGCTTGAAGTTACATAATCATTAATAACAAATTTTTTGTTTATTTCATTTTCTTCTTTACTATCAAAATTAGCTTTTAAATTTTCTGCTTTACTTATTTTTGATATTTGGTGGTATTCTTCTGGCGTTGAAAAAATTTTAATAATGTTAAAAAGTGCAGGTGTTGCCATAAATACAGGTAAAGTTATACCAAGTAATGCTTTAGATGAAACTTTTGAAAAAATAATTGAAATAAATGAAATTACAAAAGTTCCAATAATAAGAGCTTCAAAAATAAAAGCTATATGTAAAAGTCAAATATTACCAATAAATTTGGTTCCTTTTAGATCATCGGATAATCCTAAAGCTGCAAAAGATATAATAATTGCTGAGAATAATGCATTAATAGTAGCATTAATTACATTGATTAAAAATGCACTAACAAAAGAAGATCAAAACAGTTCAGTTCTTGATACTGGTTTGGAATATAAAACATAAGTTAAGCCTTCATTTTGAGGCATCTTAAAAATATAAGAACCCGTAATAATTGCGGCCAATATAATTAAAATAGGTGACATGCTTCCATAATAAATAACTATATCTCGAGCTGAATTTGTGTGAGATACAATAAGAATAATAAGGGTACCTATTAAAAGTAAAAACGGTACTAGGAAAAAGTTAATAATTAATATTGACTTGTCTTTGTATGCTGTTTTAAAGTTTAACTTTAATAATTTTCCCATTTTTTCCTTTCATATTTGTATGCTACTATTTTACTAAATTATTAATAAAATAGTCTAATTTGGAAAAAATAACCACAAATTTGAATAAAAAAATGTAGCTTTCTTGCTACACTAATTATTAGAATGAAAATTACTACTATTTTCTTAAGTTTAATTCAGCTAAAACTTTGTTGTATGTTTCTAAGTCATTTGACTTAAGGTGTTGTAATAAAACACGACGTTTTGAAATTTTTGCTAAGAAACCACGACGTGAGTGGTTGTCTTTAGGATTTGCTGCAAAGTGTTTTTTCAAATCTTCAATTTCTGCTGTTAAAATTGCGATTTGAACAAATGTGTTTCCTGTGTCTTTTTTATTAGCACCATACTTAGCAACTAATTTAGCTTTTTCTTCTTTTGTAATCATATATCGTCTCCTTATATATGTTCTAACCTAGCTAAAATCTAATATCTTAACCAAGTATAAAATTTGAATGCATTTTTTAATGCCTATAAATAATAGCACATTAATAATAAAAGCAAAATAAAAACATTTTGTGACCTAATCGTATTATTTTCAAAAGTTAGATTTTTAAAACCTGGATGTTTCACTTTTGGAATTAACAATAATTAGTAAAAAATATTAAAAAATTTTTGAAACACACGGCAAAGTTGTTATATAATAGAAATCACTATATTTCATTTATAGTATTTAGGACATTTATTAAATAAGAGAAAGGAAACAAAAATGCCTACAACAAATCAACTTGTTACAAGTGGTAGAGTTTCTAAAGTTCGTAAGCAAAAAGCACCTGCTTTGAACTTAAGTTTCAATACACTAACAAAAGAAGGTCGTAAGTTGCCAGCTCCATTTAAACGTGGTGTATGTACTCGTGTTGCAACTATGACTCCTAAAAAACCTAACTCAGCTATGCGTAAATATGCTCGTGTTAAGTTATCAAATGGTATGGAAGTTACTGCCTACATTGGTGGTGAAGGACACAACTTACAAGAACACTCAGTTGTTCTTATTCGTGGTGGTAGAGTAAAGGATTTACCTGGTGTTAGATACCACATCGTTCGTGGTACTCAAGATTTAGCTGGTGTTAATAACCGTAAACAAGGACGTAGTCTTTACGGAACTAAAAAAGAAAAGGCTGCTAAATAGCCTACTAATATAGAAGGAGAAAATTATGTCAAGAAAACATTCTGCACCTATAAGAGAAGTTTTAGCTGATCCAGTTTTTAACTCAGTATTAGTTACAAAATTAATTAACACTATTATGCTAGATGGTAAAAAATCTATCGCTCAAGATATCCTTTATTCAGCTTTCAATATCATTAAAGAAAAAACAAACAAAGAACCAATGGAAGTGTTTTTAGCTGCTGTGGAAAATGTTACTCCACAACTTGAAGTTAGAACACGTAGGATTGGTGGAACAAACTATCAAGTTCCAACAGAAGTTTCAGATCGTCGTAAACAAACATTAAGTTTAAGATGATTAGTGCAATATGCACGTCTTAGAAATGAAAAAACAATGGATGTTAGATTAGCTAACGAAATCATTGATGCATCAAACAAAACAGGTGGCGCAATTAAGAAACGTGAAGACACACACAAAATGGCTGAAGCTAACCGCGCATTTGCACACTTCAGATGATAATATAAGATATTATGGCAAGACAATATAAATTAGAAGATTACCTTAATATCGGTATTATGGCCCACATTGATGCAGGTAAAACAACAACAACAGAAAGAATTTTGCTTCACACAGGTAAAATTCACAAATTAGGTGAAACCCACGACGGTGCTAGCCAAATGGACTGAATGGCCCAAGAACAAGAACGTGGTATTACTATTACTTCAGCTGCTACAACAGCATTCTGAAAAGGAAAAAGAATTAACATTATCGACACCCCAGGACACGTTGACTTTACAGTTGAAGTTGAACGTTCACTTCGTGTATTAGACGGTGCTGTTACCGTTTTAGATGCTCAAAGTGGTGTTGAACCTCAAACAGAAACAGTTTGAAGACAAGCTACAAACTACAAAGTTCCTCGTTTAGTTTATGTAAACAAAATGGATAAAGCAGGAGCTGACTTCTTTGCAGCTGTTAAATCAGTTAAAGAAAGATTAAACGGTAATGCAGTTGCAATTCAAATTCCTATTGGAGCAGAAGCTCATTTCAAAGGTTTAGTTGACCTTGTTGAAATGAAAGCTTATGAATACGATGGAAAACCTGAAGAAAATGCAAAAGAAATTGTTATTCCTTCAGACTTAGCTGAATTAGCAAAAACAAAAAGACAAGAATTAATTGAAGCTGTTGCTTCATATGATGATGACTTTATGATGCAAGTGCTTGAAGGTGTTGAACCTGATTTAGCAACACTTAAAAAAGTTATTCGTAAAGCAACCCTTACAGCTGAATTCTATCCAGCAGTTTGTGGAACAAGTTTCAAAAACAAAGGTGTTAAGAAAATGATCGACGCAGTTGTTGATTACTTACCATCACCTTTAGATATTCCAGCTACAAAAGCTCACTTAGATGATAAAGAAGTAGCTATTCCAGCTACAGATGATCATGACTTTATTGGTTTAGCATTCAAAGTTATGACTGACCCTTATGTTGGTTCATTAACATTCGTTCGTGGATATGCAGGTATTCTTAATAAGGGATCATATGTATATAACTCAACTAAAGATGTTAAAGAAAGAATTGGACGTTTAATCCAAATGCATGCAAACAGCCGTCAAGAAATTGATGAATGTTATGCAGGTGATATTGTGGCAGCTGTTGGTCTTAAATCAACAACCACAGGTGACACATTAGTTGCTGAAAAATCTCCAAAATACATTTTGGAAAAAATGATTTTCCCTGAACCGGTTATTTCACAAGCTCTTGAACCTGAAAGTAAAGATGCTATGGAAAAACTTGCTTTAGGACTTCAAAAATTAGCTGCTGAAGACCCTACATTTAGAACATACACAGATGAAGAAACAGGTCAAACAATTATTGCTGGTATGGGTGAATTACACCTTGACATTATCGTTGACCGTCTCAAACGTGAACATGGTGTTAAAGCTAAAGTTGGTGCACCTCAAGTTTCATACCGTGAAACAATTACAAAAAGTGCTGAAGTTGAAGGTAAACACATTAAACAATCTGGTGGTAAAGGTCAATATGGTCACGTATGAATCAAATTTGAACCAAACCATGACAAAGGTTTTGAATTCGTAGACAAAATTGTTGGTGGTAAAATTCCTAAAGAATACATTAAACCAATTCAAAAAGGCCTTGAAGACAAAATGGCTGGTGGTATCTTAGCAGGATATCCTATGATTGATATTAAAGCTACATTATTTGATGGATCATACCATGATGTCGACTCATCAGAATTAGCTTACAAAATTGCTGCATCTAAAGCTTTAACCAAAGCTAAAGACTTATTAGGTACAGTATTACTTGAACCTATTATGGACGTTGCTGTAGTTATTCCTGAAGATTACTTTGGTGATGTTATGGGTGACTTAACACGTCGTCGTGGACAAATTGAAGACAATGAAACAAGAAATGATGGAGCTCACATTATTCGTTCATTAGTTCCTCTTAAAGAAATGTTTGGTTATGCTACTGACTTACGTAGTATGACAGCTGGTCGTGGAACATACCAAATGCAATTCCACCACTATGAAAAATGTCCAAAAAATATTGCAGATGAAATTATCAAACGTCGTAACATTCAAGTTAAAGACGAAGATTAATAATACAAAAAATGAACTCATAAACAAAAAACCGAGTTCATTTTTTTATATCATAGATATAAGGCAAATTAGAATAATCTAAAATGTATATTTTTCCAGAATTATGAATAATTTGATTAATAAAAGGAGTAAGTTATGACAGATAAACTTTTTGAAAAAAGCAATATTTGCCTAGACTTGTTGACCAAAAAATAAAAGATTCCTTAGAAGTTATTGGAGGAATATTTTTGGTTGGCCCTAAATATTGTGGCAAAACATGAACAGGAACTAATTTTACTAAAAGTCAAATAAACATACTAAAAAACAATTTAGACTATGTATTTGATTTAAACAATACCCTTCAAGGAAAAACACCTCGACTTATAGATGAGTGACAAATTAGACCAGAAATTTGAAATGCTGTAGTCAATGAAATTAGTGTTAGAAGAATATATTTTAACTGGATCAATACTAAGTAACTGAGACAAGATACAACACTCTAACGTTGATCGTATCGGAATGTTAAATATGTATACTCTTTCTTTAAGTAAAATGAATTTAAGTACTAAAGAAGCTTCGCTTTCAAAATTACTAAATGAAAGTAAACTTGAAACTAAAACTTTAAATACTCCTTTTACATATCAAGACATTGCCCAAATTGCTTGAAAAGATGGATGACCTTCTTTAATTAACAATAACTCAAATGCTCTAGTTCAAATTTGATTTGATTACTATATTAACAATATAATGAATCTTGATGAATAATATTTTAAAGCTAGAAAAATAGACTTTGACAAATACAATTTATTTCTTAAATCACTAGCAAGAAATGAAGGTCAAACAATCAAATTAAGTGCTATTGCAAATTATATAAATAATGTTGCTAAAATTGATACAAGAACAATTGATGATTATTTGAATATTGTTTCAAAATTATTTATTATTGAAAAACAAGAATCATATATTCCAAATTATTTGTCAAGTAAAAAAGTTTTAAATACTGCAAAATTAAGATTTATTGATTCTTCTATTCCAATAAACATTTTAGGCTTAACACCCAAATTTCTTGTTGATAATCCAAACATGTTAGGTGTCTTTTTTGAATCATTAGTTATTAGAGACTTGCGCGTTTATGGTCAATTATTTAATTGAAATATTTTACATTATAGAGATAAATGGTCAAATGAGGTAGACTGTATTTTGCAACTTAAAAATGGAGACTTTGCAGCCATTAAAATTAAATTAAGTAATAGTTATGAAGAGCAAACAGCTACAGGCTTACTTAGGTTTAAAAAAGAAATGGAAGAAGAACAAAAATTAGCTAAAATCAATAGGGAAAAAATGTACAAAAAAGTACCTAAATGATTAATAATAATTAGTGGGACAGCTCAAAAGGCATATCAAAGAGAAGATAAAATTTTTGTAATTCCATATAAAATGCTTTGTGAATAATTTTGCTTTATAAGCCTATAAAACAGCCAAAAATAAAAAAGACCATTATCTAAAAATGATCTTTATTCCTTTGGCTTTTGATTCATTAGAAACTATGTCCATAATTTCTTTTTTAATTCTTGGATCTAGATTAATTTTTTCACTTTCAAAATAATTTTTGAACATTGTACGATCATTATGAAAAAGAAAAGTCATATAATAAATTTCAGCTGTTACTTCAGGGAATTTTATTAACTTTTTCAAGATTAGAAATGATTCAACTGTAATACTTAAATAAACAATTACAACTATCATACAAAATGCTGCTGCAGTCGCCGCTAAATCTTTAGTTTGCTTCATTATATTATTGTCATATTTATACTCTAAACCTTTGTAAATTGGCATAATAAAGATGCACAAATAAAAGATAAAAGCCATTAAACATGAAGCAATAATGTTATATTTTGAAACGCCTTTACGATCAAATTTAATTCAAAAATAACAAGCTAAAATAAGCGTAATTAATGAAATTATTGAACTAGTTGCAATATAAACAAAAAAATTATATCAATAATTTAAACCACTTAAATAGTAATCATAATTCTTTAATGCTAAACTCAAAAAAGTTACAGTAATAGTAAAAGCTACTAATAAAGAAATAAAAGCAAAAAGAGAACTTCAAGACAAATCTCTTCTTAATTTAACTTCTTCTGTGTGATATTTTTGAGCTTCTTGATTAAAAGTTTCAAGCATTTTGTATACCTCTTAACTAATTTTATAAAAAATAATTTTTTTTAATTGAATTTAATTTATTTTAGTGTATTCTTATATTAATTAGAAGCCAAAAATAGGCTTCAAAATAATAAATATAAAGCATAAAGAGTTGCTGAGCTAGCTGGCTTTGACGCATGCCAACCTGCATTTGTAAGGTGGACAGTGAGGGTTTATTAACTAAACTAACATGTTTAATTATTGAGCTAATAACCTCTAACTTTTTATGCAACAAAAGTGGAGGTTTTTTATGAACTTAAATAAACAAATTTTATTCACAAGCGAATCAGTTGGACAAGGGCATCCTGATAAAATTTGTGATCAAATTAGCGATGCAATTTTAGATGAATATTTAAGAAAAGATCCTTATTCTCGTTGTGCTATTGAAACTATGGCTACAGGTAATATGCTAATTATTGCAGGTGAAGCAAGCTCAAAAGCAAATGTTGATCATAAGCAGATTGCGGTTAATGTATTAAAGAAAGCGGGTCAATATTCAGATCATTTAAGATTCCATCTTGATGTTAAAAAACAAAGTACAGATATAAGTCAAGGTGTTATTAGAGCCAATGAAGAAATTGGTGCAGGTGACCAAGGAATTATGTTTGGTTATGCAACAAATGAAACAAGAAACTATATGCCTTTAGCTATTACATTAGCTCATGAAATTGTTAAACGTGCTGATAAATTAAGAGAACGAGGCCTATTTAAATATGCTAAAAGTGATATGAAAAGTCAAGTGACTTTAGACTATACAGATAGTAAAAGAATTAAAGTTGATACTATTTTATTTTCATGTCAACATGAAGAAAACTTTAATGAAAAAGAATTTAAAGAATATATTGTAAAGAATATCTTTGAGCCTGTTTTACAGGACTATTCGTTAAAATTGCCTGTTAAAATTTTAATCAATCCTACAGGGCGTTTTGTTATAGGCGGACCTATTGGAGACACTGGCTTAACAGGTCGTAAAATCATAGTAGATACTTACGGTGGAGCAGCTCGTCATGGTGGCGGAGCTTTTAGTGGTAAGGATGCGACTAAGGTTGATAGATCAGCAGCTTACGCATGTCGTTATGTAACTAAAAATTTAGTTGCTGCAGGTTGTGCAGATAAAATTGAATTACAAGTTTCTTATGCTATTGGTGTTGCTGAACCTGTTTCAATTTTAGTTGAAACTTTTGGTACAGAAAAAATCGCAAAGGATAAAATTGTTTCAATTGTTCATAAACTTTTTGACTTACGTCCAAAACACATTATTGAAAGTTTAAATTTAAGAAGACCTATTTATCAACAAACCTCATACTTTGGACACTTTGGTCGTGAAGATTTAGATCTCCCTTGAGAAAAATTAGACAAAGTTGAAGCTATCAAAAAATTAATGAAATAAATTATTTCAAAATTAAAAAATAATGTAAAATAAATACACTTATATTTTTGATTTTAATACACTAAGGAGGAACATCTTATGCGTAAAATTGTTAGAGCAAAAAACAAAGAACGTGCAGCTAAAAGACTTCACAAATTACAACTTGAAGAAGCAAGAGATAAAAGAAGAGCAGCAAGAGAAGCTGAAGCAAAATAATAATCATAATAATAAGCAAGGAACAATTTTGTTCTTTGTTTTTTTATCCTTTTTTAGGAAGATATTATTAATTTTGAATTAAGTTTTTTAAACTTGGATGGTTCTCACTTTTATAACTTTTAGAATTGTGGTTCAAAATATAATACCTATTGATTTACTTGGAAATCTGAATATTGAAATTCAGGTACAAGATATTAAAACTAATGAAACATCTAAAGTAAGAAAATTTAATTTACTTTTTAAAAGTTTGCTAAATAAAAATAAAAGAGCCTTATAAAAAGGCTCAAATATTATTAATTAAGTATGAAATTATTTAACTTCAACTGTAGCACCAGCTTCAACAAGTTTAGCTTTGATAGCTTCTGCTTCATCTGGTTTGATGTTTTCTTTAACAACAGCTGGAAGGTTTGAAACTAATTTGTTTGCATCCATTAAAGCTAAGTTTAATAATTCTTTAACTGCTTTAACAATAGGTAATTTTTTACCATTGTCTGAAGTAATTGAAACTGTAACTACTGAAGGACCTTCAGCAGCTGCAGCTGCAGCAGGAGCAGCAGCAACAGCAACAGCTGATGGGTCAATTCCAAATTCTTCTTTCATTGCTTCAACTAATTCCATAACTTCTTTAATTGACATTTCTTTTAATGATGAAATGAATGATTCTTTTGTTAATTTTGCCATGATTAATTTCCTTTCGTAAAATTATTTGTTTTCTTCTTTTTCACTAAACATTTTTAGAGAAAGAGAGAGTTGTTGTAATGGTGACATAAGTGAACGAGCAAGAATTGTAAGAGCTTCTTCATAGTTTGGTAATGAAGCAACTTCTTGAACACCTTTAGCATCGATAACTTTGCCTTCAAAAATACCAGCTTTAGGAACCAAAAGTTTGTTTTCTTTAGAGAATTTAACTAAAACTTTTGCAGCAATTAGAGCATCGGTTGTACCAAAAGCAAAAATGTTTGGTCCTACTAAGTGTTGATCTAAATCATTAATGCCATGATTTTTAGCAGCTATTTTGAAAAGACGGTTTTTGTAAACTTTAATGTCAATACCTTCTTTTTTAGCTTCTTGACGTAATGAAGTCAATTCAGCAACACTTAATCCACGATATTCAGCTATAACAAGAGCTTGTGAAGATTCAAGTTTATTTTGAATTTCTTTAACTGTATCTTTTTTAACTAATCTAAAAGCAGATTCATGTCTTTTTTGCATGAGTATCCCCCTTTCATAATTTAATATGCAACACGGGACAATAATAATGTGATAGAAAAAAAGTAATTCTACACTCGGTAACGAATTAAGCTTTCGCTGTTACTGTCTTTATGTATTGCGTTTTTATTATATTAATAAATTTAAAAAATCAAAGCAATTTTTTTACTAATTTTAGCCAGTTGATTTTAATAGAAAAAATAAAAACAGCAAGTAAGCTGCTTTATTAATTTTTTTCATTATCAGTTAAGGTTTCATCAGTATTTAAAATGTCTGCTTTATTACCTAATTCAATATCTACACGGACATTTTTGAATCTGTTAGTTCCTAAGTCTATAACTTTAAAGTAAACATCATTGTATTCATATTTTGTATTTTTATAAAGTTTGTGGCCGAGTCTTTTTTCTAATCATTTATGAAGTGTTAATTCATTTTCTTCTTCTTCAAGATCTAATTCTATTTCTAGTTTTTTAATTAATTCTTTCATTGTTAAAGAAGCTGAGACATGGAAGTGTTCAAGACTAATTTCAAAAATGTCTTTTAATTCTTCATCATCAAATTCATCATAAATTTCGCCAACAATTTCTTCAATAATGTCTTCAATTGTAATAATTCCAATTATTTCACTTGAGTTGTTATTTGGTGTAACAAAAGCCATTTGAGCTCTTTGGTACCTCATTTTTTCTAGAGCCGAAGAAAGAGAAGCATTAGCACTAATAGTAGGCACAGTTTTAAAATAGTTTATAACTTTACCTTTTTGTAAAAAGAAGACATCTTTTAAGTGTAAAATGCCAATTAAATTTTCATCTTTTTTAACAGGAATACGGCTATAGTTTGTATCTTTAAAAATTTCAAATGCTTCTTGAATATTAGCATTAGCATCAATTATAGATACATCTTTTAATCTTATGTAGTGTTTTTTAACTTTGGTACTATCTAAGTCTAAAGCATTTTGAGCCATGATGCTTTCATTAGCCTCTAAAACGCCTTCGTTTTGCGCAATATCAATAATATTTTTAACATCATTTTCTGTATTTGTTATATAGATTTTTTTACCAATCTTGCTTATTGGATAGGTAATTGGTCAAAACAAGTAATATAAAATAATTAACAAGTAGCAGAAAACTTTAACTGTCGCTTCAGGTTTACTTTTGGCTATTAATTTAGGTGTAATTTCTCCAAATAAAACAATTATTGGAGTCATTACAGCAGTTGAAATTAAAACGTTATATGATTCGAATTTTTTACCTAAGTAAGTTGATAAGAGTCAGGATGTTAAGGCCGAAGCAGCAATATTAACTATATTGTTGCATATTAAAATAGTACTTAAAGTTTGGTTGAAAAATGTATGTTGTTTTTTGATTATTTTGTGGCCAAATTCTTTATTGCCAACCATTGTTTCAATTTTGCCAACATTTAAGGATGTATAAGCAGTTTCACTGGCACTAAAAATTCCGCTAGCTATTAACAAAATAACTAGTCCGAATATGCTTAATCCTAATTTCAGTTTCGGGTCCATGATTATTCCTCTTCTTCGTCATCATCACTTGAGACGGCAATTTGTTCATTTTCAAAATTAATGAAACGACCACATGACATTTTGAATCATAATGGAAAAATTCCTGTTGGTCCATTTCTGTGTTTTGCAATAATGATATCTATTTTTTCTCCAATATCTCCAAAGAGATCTTTGGTTTCTTGTGAGACTTGTTTTTTATAATAATTTTCACGATATAAGAACATAACAATATCGGCATCTTGTTCAATGTTACCTGATTCACGTAAGTCAGACATAATAGGTCTTTTGTCTTCACGTTTTTCAACTTCACGGGAAAGTTGGCTTAGTGCAATAATTGGAACATTTAATTCTAAGGCTAATGTTTTTAATGAACGGCTAATTTTAGCTATTTCATTTTGCCGACTTTCATTTCTTCTATTTGAGTCTGATGAAATCAATTGTAAGTAGTCAATAACAATTAAATCAATTGGACTAACTTTGTGTAAACGACGACATTTTCAAAGCAGTGTATTTAATTCATTATTTACTGATTCATCTATAAATAAGTTCATTTTGTCAATTACATCTTGTTTTAAACTGTTGATTAAAAGAAATTCATTATCTGTAATTGTTTGAGGTTGTTTTAGTTTTTGAAGTGGCACATTTGTGTTCAAGCTATAAATTCTACCCATAAGTTGGTTTGATGACATTTCAAGCGTAAAGAAGACAGCTCTTCTGTTTTTTCTTTTAGCAACATTTGAAGCAATATTAAGAGCAAAAGCAGTTTTACCCATTGCTGGACGAGCAGCAATAATAATTAATTCACCACCATGTAGACCTTGAGTAATTTGGTCTAAATTGTCGTAACCTGTTGCTAGTCCATTTAATTCATTTAGATCTCTTGAGTGGCGATTTTCTAAATCTTTAAAGTATTCATCGCTAACTTCTTTGGCTGTTAAAAAGTCTGCACTAACTTGACTACGATCAATGTCAAGTAATAGGTTTTGTAAATCTGAAATAACATCTTTTTCATTGATTGCTTTTTCAACATCAAGTCTTCTTTGAACTAAAGCTAAACGTGATTCAATTAAACGTAATTTTGTTAACTTAACTAATTCTTCTAAGTAAGTTTGAATATTAGATGAGAAACCAGCAGTATGATAAAGTTTAGATAAATATAAAGGTGTTATTTGAACAAAGTTTCTTTTTTTAGCAACAGTAAAAATGTTTTCTTCATTAATCGAAATGTTGTTTTCAAATAGTTCTTTAAAAACAGAGAAAAGTCTTTGATTTTCTAAAATTGCAAAGTCTTCTTCAAGTAAGTAAGGCACAATTTTAGGTGCGTTTTTGTTGTCTGTTAAGATTAAGCCTAAAAGAGACTCCTCATATCTAGAGTTAATATGACGAGATGTATTTATTGGTGCATTACTTGGATTATTATTTGGCATTAATTATCACTTCTACTTTCAATTTTGCAATGATATCTTTATATAAAATAACATCTATATCATGCAATCCATTAGATACTAAATGAACTTTTTGAACTGCGTATTTATCTAATTTATAGCCATTTTGGACTAAATCTTTAACAATATCTTTGGTTGATACAGCACCATGAGCAATTAAATTTCCATTATTGTCAATATGCGCATCTAAAGTATATTTTAAAGTTAATTTTTCAATTTGATCTTTAAGTTCAAGAGCTTCTGAACGTTTTTCCATTTCATTTTTAGTAAGCTCACTAAGTCTTTGTTCAAGCTCTTTTTTAGTCTTGTCATTATAGACAACTGCAAAACCTTTTGCAATTAAGAAGTTATTACCATAACCAGCTGAAACTTCAATAATTGTATTGGCTTTACCTTTATCACAATCTTTAATCAATATTACTTTCATTTTTCACACTCACTATCGCTTGTTTAATATTATCTACAAATGTCAACAAGTCTTCTTTTGAAACTGCAGCTGCTGTCCCAAAGTGTCCACCTCCACCAACAGCTTCGCAAATAATTTGAACATTTGTATCAATACCTCTGGCACTTAATTTATATGATTTTTTGTCTTCACTTTTAGCTACAACAAAACTTGCTTTACGACCACTAATTCTAAGAATTTCTTCAGCTGCTATAGAAATAACATCATTCGAAAGTGGAATATCTTTGTAAGCTAAATAGAATCCTGGTTTTACTTCTTCTAAGTTTTTTAATACTTGATCAACTTTTTCATAAGTGGTTGCATCAATTTTTAATGAATTAGAACTTTCAGTTGAATCTGCGCCTCTATTTTTAAGTCAACTTGCTGCTTCAAATGTTCTTGAAGTAACATGTTTTTGAAATTGTAAGGTGTCAAGATAAATACCATTTAATAACATTTGAGCAATGTTCTTTTTAACTTTAACTTGCTTTTCTAAAAACATCATTAATTCAGTTACTATTTCACTAGCACTTGAAGCTGAAGGCTCAATGTGGCGATTAATTTTTGAACAAAAGTCAACTGAAGAATTTAATCTATGGTGGTCTAAAATAAAGATATTTGTGTTTTTAGCTTTTTCAACACATTTTGGATTATCAGTCCTATTTGGAGCTGAGTTATCAACTAAAAATACAAGTGTATTTTCATCAGTCAATGTGTTAGCTTGATGAGCTTTAATGAAAATTGGATTATCTTCACCAAAGTATTTAGCAATTGCTTTTTTAGTTGTAACATCTTGAGTCATTGTGCAAATGTAAGCTTCTTTATCAAATGTTTTAGCTATAGCATAAACTCCTAAAGCTGAACCGATTGCATCCAAGTCTGCATGTGCATGACCATAAGCAATAACTCTTTTAATTTTTGGATCTAAAAGTTTCTTTTCAATTCAATTAGCAAGTGCTTTGATTCTTGCACGGTTGATATTTGGCAAGATTTCACTTGTTGAACCAAAGTAACGAGGTAAATTATTTCTTGAGAAAATAGCTACTTGGTCACCACCACGACTTTGAGCTTGTAATAAAGCATTTTTAGCTTGTTCCATTTTTTCTCATAAGTTTTTTGTTCCATATGCAAAACCTGCAGAAACTGAAATAATAATGTTTTGACTTTTTAGTGTTTTATGTAAATCATCAAAAAACTTAAAGTTTACTTGACTCATTTTATCTAAAGCTTGCTTGTTAGTTACAATAAGAAATTTACCGTTTGTATTATATTGACGATAAACTAAGTTGTAATCACACACTAAGTTATCTAATACTGTAACAACTTCTTTAGTTACATTATAAAGTTGTTCTTCAGAAAGAATTGATTGATAAAGCTGATAGTTATCAATTTCAATTTCACCTAAAACTGTTAATTCATCTTCATATAATTGAAGAGTTCTTTGTTCAAGAGTATTGTCCTTAATACTTAAACAATTTTCAAGTGGTCAAATATTTACTGTATATGAAAAATCTTTGTAATTAAAATCAAAAGAAATGTTATTAGAATCAAAATTAATATTAAATTTCTTGAAAAAATCAGTTAATTTTGATCCGACTCATTTTCTACCAAAACGGTTTTTAATAAATTTACTTGTTCAAAGAATTTGGCCTTCACTGTCAAAAATAAGCACTCCAATTGCATTATGACTAATGATTTCATCAATGAAACTGTTAAAAGATTTTTTTACTATAGCTTGAGTTGATAAAAAACTTTTAACAGATAAAATTCCAAATAATCCAACGATTATTAATGAAAGACATATGCCAAAAATTCCAAAAATGCTGCTATAAATATCTGAACGAGTAAAATACAAAATTACTGACCCTACAGCTAGGAAAACGCCAGCAACTGCTATTAAAGAATATAAAATTATTTTTTGTTTTAAGTTCATAATATAAATTATATATTAAGTATTAATATTCATTTGTAATATATAAAATTTTTGACTTAAAATAAAAATAATCATGAAAAAAACAACAAAAAACTAAAAATGCTATTTTATAGTTCTTTTACTTTTAATTATATTTCTTTTCAATGTTTGAAAGCAAGCTTTTTATGTCTTGCATTTTGTCTTTAAATGTTTCTTGAGTTTCTTCAAATTTGTATTCATTCAAAATAAATTGATAGTCATCTTTGTCAATTTTTAAGAATTTTTTTTCTTACTCATTTTTTTGATAACTACAAATTTTAGTATTTTATCATCTGTTGTTTTTCTAAATTCATCTATAAAAAATTGGAGTTTGTTTTCAATATTTAAATCAAGCATTTCATTAAAGGAATTTATAAGTTTTTTAGAATCATTATTATTGTGGTCAATATTAGCCAAAAGCAGAAAATCAGATTTTGATCTAGAAATAACAAAATGATATTCATAATCAGAGTCATCAAAATTGTAATTAGTTCGAAAAACACCTTTGATGTATTTTTCTTTATTTAAAAATTCAACAATGATTTGTATTTTTTTATTATTTATTAAATTATTTTTTGATATAGAAATTATTAATTTATCATATTCAAGATTTTGTAAAAAGTAATCATTATTTTTTAAGATATTTCCATTATTATCAAATACTAAAAAGTCAAAAGAATTAGCAGTTTCTTTTTCAATTAATAATGGGTTATTGAAGTCGATTCAGGTTATAATTTGACCTGTGTTTGCATTTAAATTTTTATTTAATATTTCATTTAGGGAGCTTTTTTCAAAGATATAGCGTTTGTCTTTTTCTCTAAAAATATACTGTGAATTGTAAGTGGTTAATTTATTATTAACTAGATAAAGCATTATGTTTTTCATTCTATTAATTAAGAAATTTAATTTATTATTTTCATGTCAAGTATTCATATTTTCAAGATGTCTTTTATTTAAAATTATTTTATAAAGATTTGAGTTTTGCATATAGAACAAATTGTCGTTATTAACTGTAAAATAGGTTTTTTTGTAAAAAGTGTTATCTTTTGGAAAAACATTTGTTAAGCTATCTATAAAGTTTAAACAATCTGAAAAATTGTTATAAAATTCTAGATTTGCTTCAAATTTTTCCAATCTTTTTCAATACAATTCATAACTAGAAACAAAGTTATTAAAAGCAAGAACAAAGTTGGTATGAACCTTAAATATTGGACTTTTAATTATGTTTGGACTTATTCCTGTATTGAACAGGGCTAAGTCCTTTTAATTTATTAACAATTCTGTCATTATTATAATATGAAATATATTCTTCTAAAGCGGTTTTAAATTTATTTTCTTCCCCAAATCAAAATTCTCTTTTTACAACACTAAATAAACATTCAGCGGGACTATTGTCTAAACAATTTCCTTTTCTTAACAAGCTTTGAATTGTTTGTTTCTTTTTCAAATAATTTATATACTCTTCATGAGTATATTGTCATCCTTGATAAGAATGCAATAATACATTGTTTAAGCTATGTCAATTCTCTTCTACGTTTTCCAACATTTTACCCGCCATTCTTAAGTTAGTTGATTTAGAAATCGAATAACCTAAAATCTCTCAACTTTTAAAATCAACCACACGAAAAAATATGCTTTTTCATCATTTTTTAATTTAAATTCAGTAACATCTATTCCTAGTATTTTCAAATATTTATCACTAAAAAAATTTTTTTGTATCTGAAGAAATATTTTGCGATATTTTCTTTATCTAAAAGCAAGTTTGGAATGTTTTTTTGTGAGTATTGCCTATGAATGAAGAATACTTATTTTTTTCTTAATGTTTTGCCAAAAAGATTTAATGATTTTATTAATTTTTGAACCTTTTTATGATTAATCATTATTCCTTTATTTCTTAATGCAAAAGTAATACGTCTATAACCGTATAGTCCTTTATTATAGTTAAATAAGTCAAATATAATCTTTTTCAAATTTTCATCTTTGTCAGGTTTATTTTGTGATTTTAATATCTCATAAAAAATTGACTTTTTCTAATATTAATTTAATAGAAAAATAACTTTTTAAATTCTCGCCTTAGTTCAATTATTATTTGGAATTTTTCTTTGTTAGTGAGTCTTTCAATTGAACCAAAACGCTCACTTTTTTTGCTATCGCGTTTTCCAATTTAAGTATTTTATTTTCTTCCTTTAATTGTTTAATTAATAAATCTTTATCTTCCATTTTATTTTCAATATTGATTTTAAAATTTGTTATTTTTTTAAATTTAAATTGAATAAAAATGAGCCCCTTTCCAAAAAGTCTGGAATTTGGGGTTCACTCTAAAATGACAAAATTAAAAACATTTTTTAAATATTTTAGATGAATGCAAATTTATCAATTATATAAATGTTGGATATGACTGTAGTAAATTAATTTTAATTTTTCAAAGTAATGTTAGTCAAGATAATTATTCAAATAACAAAGATAAAAATAGCCAAAAAGAACAAGAAAAAATGATTCAAAAATTTAGACCTGAATTAGTTGGACGTTTTAAAGATATCTCATTTTTGGATAAATTTAATGCTAAACAAGTTAAGGCTTTAATTAACAAAAAAAATCAAAGAAGACGAAATCAATATTTATTGTGAAAAAGCTATTTCAAATTTATTTATCGATATTAAGAAATCAGATAAATTTTATGATGATGAATTGAAGCTTAAAATTTATGCTATTAAAAATGATGGTAGTGGCAAAGTATTCAGAACCAAAATTTACTAATTTTAAGAATTTTTCAGAGCTTATTTATAAAAAATAGGCTCTTTTTTATTTGCAAATAATATCCACTATTTTTTGTTGTATAATACAAAAACTATGGATAAAAACCAAAAAACATATTTTATAGACTTAGACGGGACTTTATTCGACCAAAGGAAAAAAAGTAGAATCAGTGCTAAAAATATTAGTGCAATTTTATTACTAAAAAATTTTGCTAATGTGGTACTTTCAACAGGGAGAAGTTTTAGTGATCAAAGAGTCCAACAAGCAATTTATCAATTAGGAATCAAGGATGTTATTACTTCTTCTGGAGCTGAAATTTATATTGATAATAATTTAGTTTGACAAAGAACATTCAAAAAAGATACAGCTAATAAAATTCAAAAATTTGCTGAAGAAAATAAGATTTTATATGTAATTTTCGATGAAGAAGGCGAAACAATTTATGTCCATAATGCTTTTGATTATTGAATCAATAAATTGTTTCTTTCTAAAAAAATGCATGGTATTAAAAGAAGTAAAAACTTCAATTTTAACAATCACAAGAACATTACAAAAATTGCTTTAATTTTAAAAAATATAGCAGGCGCCAAAAACATATTAAAAACATTCCAAGTTAATTTTGATGATATTTGCAATTCATATTTAGCTAGTGCTAATTATGTTGTTGAAATCACAACTATTGATGCTAATAAAGGATTATCTGAATCTGCTTATTGTGCATTAAAAGGTATTAATCAAAATAATACTATTCATATTGGTGACTCAATGGCTGATGCTTCAGTTAAAGGTTATGTTGGAAAATTAGTGGCAATGGGTAATGCCACTAGTGATCTTAAAGTTGTTGCAGATGAAATAGCTCCTAACTACAAAAGTGGTGGTTTGTATCGTTATTTTGTTTCAAGTAAGGGCAAGAAAGGAAGCAATAATGATTAAATTAGGTAGTCACATTTCTTTTAAAAGTCCAGATTATTTAGTTGGCTCAATTAAAGAATCACTTAAAAATAAAGCTAACTGTACCATGATTTATTTGGGCGCTCCTCAGAATGCTAAAAGAGTTGATACAAAAGTTTATAAATATGAAGAATATAAAGCTAATTATTCTAATTTAATTAAACCAGAAGACATTGTAGTTCATGCTCCTTATATTATTAATCCCTCAAGTAAAGATAAGGTTAAAAATGCTTTTGCAGTTGATTTTTTAATTAAAGAAATTCAAAGAATGAATTATATTGGAGTTAAATATTTAGTACTTCATCCAGGCGCTTATACAACTTATGAATTACAAGAATCACTCGCACAATTAGTTAAGTCTTTAAAAGAAGTTTTAGCTAAAACTAAAGATGTTGTTATATGTATTGAAACTATGTCTGGAAAGGGTACTGAAGTTGGTACTAATTTTGCCCAATTACGCTATTTAATAGATGAATTAGCAAATGACAGATTACAAATTTGCTTGGATACATGTCATGTTTGAGATGCAGGTTATAATATTAAAAATTATCAAGAATTTAAGAGTGAAATTAATAAATATAATTTACTAAAACATATAAAAGTTATTCATTTAAATGATTCAAAAAATGATTTAAATTCACATAAAGATCGCCATGAAAATATCGATAAAGGTTTTATTGGAATTGATACTTTAGCTAAGTTTGTTCATGATAAAGATTTTGATAATGTGCCAATAATTTTAGAAACTCCAATTCCTGAAAATGGACCTATTTATGATCAAGAAATAGCTGCTTTATTAGCTTACAAAAAATAACAAAAACACCGAGAAATTCTCAGTGTAATTTTGTGCTTTTTTACCGTATTTTTACCATGAATAGCTTTTGCATTATTTAATGTACTATTTGCAATTTCATTAATTAATTTATAGTTTTCATGATCACTAGTTTGATGAATTTAAATTAATTCATTTAACTTTTGAATTTCTCCATTCAATCATTCCTTAGTTTTTTTAATTTCATCACTTTCAATATTAGGCATTTCAGGTTTCTAAGCGCATCCTGTTGATATTGCTATTGTTGGTAAAATTGTTGTTGAAATAGAAATAGCTACAACAAAAAATGTTTATTTTTTTATAATAATTTGCCTCTATTTTTTTCTATTATCACAATATAAAATGTATAAAAATTTAATTCTTTTCATAATAATATGAAACAGTTTTTGCAACAATTTATTGAAAAAATTACCATATTAATAAAACTAAAAAAATTAAAAATAATGAAATATTGAAATGTTTTATTTACTAATAAAAAGGTTACACATTAATAATAAATACTAAATTAGATATTAATATTTATATAAAAATGTATATAATTAATGTTATGAAAATTGAAGTTAATAGTGTTAGGAAAAGATTAAAAATTTTCGCTCGTGAAAAAAAGAATGAAAATGACGTAGTTGGCGATGTTAGTTATTCAATTCCAGGAGAAATTTTAAAATTTCTCATTTTGCTTTTTGTTGCCTTAGCTTTTATTATGTTTCAATATCAAGATCTCTTTTTTCACAATGATAATAAAGTAATAAATATTATTCGCAAAATTTATTTATATAGTTTCGGCTTAGCTTTTGGTGACATAATAACACTTGTAATAATAGGATCATTTTTAACACTTATGGTGCGTTGATTTGGTTCAATATTCAAAAATCATTATTTTGCTTGGTTTAAATCTTATACAAGAGTTGATTACTGAATTTTAAGAAAAAGAATCATCAGCTTTGTTTGGTTAACATTATTATTTGTAGTTTTAATTTATCATGTGGTTTTAGTTAGCTTCCGTGTTGAAAACATTTGATATTATTCAACAGCTGATGTTAAAAATATTTACTTAAAAGGTTGATACTATACTTTTACAAATCAAGCTGATTTATTAGATGGTAAAAGTGTTGATTATCCAGTTGCTTATTTAAATATTGGTTTTTTACTTGATACATTATTTAATCTTTTTTATGTAGTAACTTTTAGTCCTTATTTAGCAATAATTAGTGCATTTTTAATTTGAACCTTAGCTTGATTATTCCTTGTAACATTAAATCCAATTAAATATTTTAAAAACATTAACGAACACAAAAAAACAATTGTTATGGTTGAACAATACTTGCAAAAAATAAATAGTTTATTCTACTACACACCTCAAGTTAGAGAACTATTCAGATTCTACAAAGATGCAGCTCAAGTCTTAAAAGTAGATTACTTTAAATTTAATTTTAGATACTTACAAAAATTAGTTAAAAAGAATTTGACTCTTTTAGCTCAACATACCCTCACAGCTAAATACTTTGAAAATGTTAAAGCTAAAAAGACAGCTAGTCAACTTGAAGCAGAAGAATTTCTTAAAATAGTTGAAACTACTCAAACTAATACAACTTCTGCAACAATAACTTTAAATGCAGCAGCTGATATTAAAATGCCAGCAATCAATGCTCAAAATCTTTTAGTTAATAAACATAATAAAAATAATACAAATACATACATTAGCAATGAATCAACAAGTGAATACTCAAAAGATTTTGATTTAACAGAAGAAAGATATATGTCTAATTATGTTGAAACTATTGAGGTCGAAGCTCAAAGTCAAGAATCAACAAAATTATTAATTGATGATTTAGAAAACAAAAAATCAGATACAGAAGAAATCAAAGCTAAAACTAAAGAAATGACTGCTAAATTTGAAGCAGTACAACCTACTGAAGAAAAAACAAAAGAAATCACAAATATTTTATTAGCAGATCAAGTAATTAGTGAAGTAACTAAGGAAGAAAAACTTGCTCCAACTATAGAACAAGTTAGTCAACAATTCTTAGTAGACACCAACGAATTAACAGATACAAATGATGTTGAAAAAGACCAAACTCAAAGCAATATAATTGACCAAAACAATAGTAAAACAGTAAAATTAGTAAAAGAAACCATCGAAAATGAAGAAAGTAAAACCTTAGAACAAGAAATAGCATTCTTAATTGATTCAAGTGAAAATACATTTGTAATGAGTGCTGAAAAGAATACTCAAAATATAACTCAAACAATGAGTCAACCTGTAGAAGTTAAAGCTCAAACTATTGAACAAAAAATAGAAGAAAATGTTGAAAACAAAAAGCAAACTATAGGTTTCATTATTCCTGAAGATTCAAATGAAACAATAATTGAAAAAGATGACGATCTAAAAACTAAAGAAGAATTTATTGCTCAACTAGACAGCATTGAAGCAGATAATTTGGCTTCAAAAGAAATTAAACTTGAACAACCAGAAAGTGAAAAGAGACAAAATTTCCAATTCACTTTTGATACTAATACAATTGTAATCAACAATGATTCAAATAAATCAAAAGATAGAGTAGATCACTTCGAAGATGATAATGATTGAGTTAGTCCAATATTAAGTGATAATTAGGAGTAAAAATGAAACAAGTTGCATGAAATGAAGTACAAAATATGGTTGCTAATAGCAACAACGAAAACAATATTTACTTTATAACCTTTACAACTTCATGATGTCCAGATTGTAAAGTTATGAAACCAATTGTTGAAAGTGTTGAAGAAGAATATAAAGAATACAATGAAATTATTTTTCTAGAAGTTGATGCTGAAAAAGCTCAATTATTTAGAGAAATCAATAACCGTTGAAAAATTTTACATGTTCCAACATTTTTAATAATGAAAAATAACAAAATCATTCAAAGAGGAGAAAACTATTATCCAAAAGAAATTCTTTGTGATTGAGTAGAAAAAGTTTTAGAATAAAAAATGACTAAAGAAGAAATAATTACTTCATTAAAAAATGTTTCAACAGCACCTGGTATTTATCTCTGAAAAGATAAAAATGATCAAGTTATTTATGTTGGAAAAGCAAAAAACTTACGCAAAAGAATGCACCAATATTTTGAAGGTGCAATTAACTCATATAAAACAAGTAGGCTTGTTGAAGAAATAAGAGCTTACGAAGTTTTTATTTGCAAAACCAATAAAGAAGCATTATTACTTGAAAAACTTTACATTGATAAATATAATCCTGAATATAATATTTTGCTTTTGGATGACCGTCGTTATCCATATTTAAAAGTACAATTAAGTGACAGGGGTTTAGATATTTCATTGTCAAGAAAAATAGGCAAAAAGAATGAAACTAATAAATTATTTTACTTTGGACCTTTTCCAATAGGTTATGGTTCTATTCAAATATTGAAATTATTAGAACGTGAAGCATTTTATGAAAAAGGTTTAAAAATCAAAAATAATGATCCTAAATTTTGACAAGAAAAGTTCAACAAAATTAAATCCATTTTAAGTTTTAAAGATAACTCTTATATTAATGAAATTAAAGAAAAAATGTTCAAAGAAGCTGAGACTGAACATTTTGAATTAGCTCGTGATTTAAAACTTTCCTGAGAATATCTTAAAAAGCTTAATGAAGAACAAATTATTGAATTAAAAGATATTCACAATTTAGACGTATTTGCCTATAAAACAGTTGATAACATAATTTATATGACAATCCTTTTTTATCGTTCAGGTCTATTAATTAATAAAGATAATCAAGCTTTAAATATTGACATTGATGAAATTGAAACACTTGAAAAATTCTTTAATAAATATTATGAAAATAAAATAAAGCCAGATGAAATTTTAGTTGAAGAAAAAATTAACAATTTAAACTTAAATTTAGATGAAAATTTAAATATAGTTACACCTAAAATAGGTGATAAAAAACGTGTTTTAGAAATAGCACAATTAAACCTTGAAGAGTATTTTAGTAAAGAACATTTACTAATTAAAAATAAAAATGATCATGCATTATTAATGCTTGAAGAATTTAAAAAGCTAATCAAAATTGATTCATTAAAAAATATCATTTTATTTGATAACTCAAATTTAGCTAATTCTAATCCTGTCGGTGTTGCTATAGTTTATTCTAATGGTTTAAAAAACAAAAATTTATACAGAAAATTTAATCACATTGATATTTATTCTAGAGAAGCAGATGTTGAATACATGAAAGAATCAATTAAAAAATACTTTAGTTCAGCAGACAACAAAAAAGATTTTGATTTAATTATTGTTGATGGTGGTTTAGCTCAAATACATGAAGCTAAAAAAGTTTTGTTTGATTTAGATATTAAAATACCAATTGTTGGTTTAGTTAAAGATGATTTTCATAGAACAAGAGCTTTAATTGATTTACAAGAAAATTTAGTTAAAATAAAAAATAAAGATTTACTAAACTTTTTGGCAAGTATGCAAATTGAAGTCGATCGCTTTGCTAAAAGCCATTTGAGAAATAGAAATCGTATCACTACTTTTGAAGGCAGCTTACAATCAATTAAAGGTTTAGGTAAATGTGCGGAAACTAAATTACTAAATACATTAAAACATATGTTAATATCTTTAACGCTCCTTTAGAAGAATTAGAAAAAGTTGTTTCTAAAAAAATAGCTTTAGAAATCAAAAAACTTTATGAAAGTAAGAAATAAGCAAGTTTATTTTGCTTGTTTTTTTAATCTATTTTTTATTTACAATAAAATATAAATAAGAATAATAAGGAGATTTTATGAGTTTTAGTAAAAAGTTTTTATGAGGTACTGCTTTTGCAGCTAATCAAATTGAAGGAGCTTATAATGAAGAAAATAAGGGTTTAAATGTCCAAGATGTATTACCTAAAGGTGCTTTAGGTCATATTGATTTAAATTTAAAAGAATTTAATATTAAAAGAAAAGCTATTGATTTTTATCATAAATATTAAGAAGATTTAGATCTTTTAAGTGAAATGGGAATTAAAGTTTTAAGAATTTCAATTGCTTGAAGTCGAATTTTTCCAAAAGGTGATGAAAGTAAACCTAATCAAAAGGGTCTTGAGTTTTATCTTAAATTGTTCAAAGAATTAAAGAGAAGAAAAATAAAAACAATAGTTACTCTTTGTCATTATGAAATGCCTTTACATTTAGCACTTAAATACAATGGTTTTAATAATAAAAAAGTTGTTAAATACTTTTTAAATTATGCTAAAGTAGTTCTAAAATACTTTAATAAATACATAGATTATTTAGTAGCTTTTAATGAAATTAATTCACTTTTACATATTCCTTTTTGTAATGGCGGAATTTACAAAAATCAAGAAGATTTAAATATTTTATATCAAGCAACTCATAATATGCTTTATGCTTATGCTAAAGTCAAAGAAATAATTAAAAATAATTACAATCAGATTCAAGTAGGTTCAATGGTATTAGCTATGCCTTTTTATACTAAAAGTGCAAAAGTAGAAGATCAAATATTGAAAATGGAAAAAGAATGACAAAATCTTTATTTTACTGATGTTTTAGCTCGCGGAGTTTATCCTAAATATTTAGATAATTATTTTTTAAAAATAATATAAAAATTAATAAGACTAAAGAAGAACTGGAAGTGATTAAAAATAATATAGTAGATTATATTGGTTTTAGTTACTGTTAATTCCAAAAGTGAGACCCCTAGGTTTTAGAGATCTCACTTTTTAAATTGTCCTTTATAATATAAATTGCTTAAAAAATAAGAAAGGACACTTAAATTATAATATGTTAAGCAGAGAATATTACACCTTTAATAATATAAATTGCATTAAAGATTACACAAAAAACATTCATTTAATCATTTTAGATGATCCAGATAGTGAATGAATTTCTAACAATTATTTTAGAAATAAAGTTCATATTATTTTCAAAAGTGAGGGTGGTAAGTTTTAAAAAATTATATATGTGATTATGTTAATAATTTAACAAGTTCATTCAAATTTTAGTTAATTTATTGAATTCTTATTTAGCTTCTCCATATAAGTCTACATTGAACTTAACATTGTTTTTTAAATCTGTTTCATAGTTTTTACATTTGAAATAAGTGAGAGGAAATAGAAACAATCCAATTAAATATATTAAGCAAACTAAAATAAAGTTAACAACAAGTTTCATAAAATAAACAAAGATAACTCATTTAACACTTCCTCAAGCTATTATGCTACTTCCTCTTCATCTAGGATAAAAACAATCCATATGATAATAAGTGATTACTCTTTCATCATTAAAGGTCGCTTTAAAGATAAAGCTTTTAAGCACTAAAGCAAAAATTAAAGGAATAATAATAATTGCAGCATATATCACTATTTGGGTAGTGCTAACAAATGTATTTGCATTGGTTGTAGTATCACGATTCCGTAAATTGAGTCATGATGCTAATGCGCCTATACCGATTGTTGCAATATTAGCAGCAAAAGCAATTCAAGATAATTTTTTTATCTTATCAATAATAGATAATTGTTCTTCCATATGAAATACTCCTATTTGATAATTTGAATAAACAAATTATAAAATAAAGAAATAAAAACTTTATTTAAATATTTAAAAAGCTTAATAAAAATTAGATAAGTAATTATGTTAAATACTTTTTTTAAAATTCTTTAATTATGTCAGGATTTTCTAAATAACTTAATAAAGAAACATGAGTTATTCCATCTTGATCTATTCAGTTATTTTTTTCGCTATAATCAACAATAACTTTTCTAAAGTTGTTGTTAGTTAAAAAGAATGGTCTCAATTCTCTTTCTTTAACTTCTTGATTGTTTAAAGAAGTTGTTACTTGAAAATATACTTTTGTATTGAAATTTTGTGCTATAAAATCTATTTCATATTGATTATTAATTCATTGATTATTAATATTATTTTTTTGATTAATTGCCCCATAACTAATATCGAAGTAATTTATTTTTAGAGCATTAAAAAAGGCGTTTTCGATTAATTGACCTTGTCCTAGATTTCTAAAATTGAGAGCTACATTTCTTAATCCGTTGTCAACAAAATAGTATTTATATTGACCCGAAAGAAGTTTTAAACCTTTAATATCATATTTTCTTATTTTATCTATTAAGAAAGAATCTTCTAGTGCATCTAATATTTTATCTATTTGATAAGTAGAAGTCTTTTTATTATTTAAAGAATTAAAAGAGTTGGCAATTCTACTTGAATTACATTGAGATGAAACTATTGAAGCAAGATATTCAACTATGTTTAAAACATCTTGAGTACTATTAAGTTTATGTCTCTCAACAATGTCATTAACATAAACATTTGTTAAAAGATCTTTTAATATTTGTTTCTTTTCTTTTTCATTATCTACTAAAACTACTTCAGGCATACCACCATAAAGAGAATATAAATTTCAAGCTTCATTTATATCAAGATTACTAAAGTTTAAATATTCTTGAAATGATAAAGGCAAAACATTAATCTCCATACCTCGACCTGCAAAAGTAGTAGCTATATCTTTGNATAAGAATTTAGAATTACTGCCAGTAACATAAATATCAATATTATCTTGATCTTGAAATGAATTTAAAACCAATTCAAACTTTTTGACATTTTGAATCTCATCAATTAAAAGAAAATATTGTTTATCGTTAACAATTCTATCTTTAATGTATTTGTATAACTTTTGACGATCTCAAAGTTCTTCATTTTCAACTCGGTCAAGTTTTATTGTTATTATTTGGTCAGCTTCCACCTTATTAGCTAATAAATAACTATAGAATAAATTGAAGAGTAAAAAACTTTTGCCTACTCTTCTTATACCTGTAATTATTTTAATTTTTTTGTTATTGATTTTATTAATTAGCATATTTAAATAATTGTCTCTTTTAATAACTTTATTCATTTCTAACTCCTTATAAAAATTATACTATTTTCTTAAAAAGTTAGCAATATTGCCAATGTTTTAAAAAAATTACTTACTTGTTTATTAGTGATATTCTATTTCACATATAGGTGGAAATGCTAAGTGATAAATTTTTAAAGATTTGACATGTCCGCATTGTGGGGCTTATAAGCCCTTAGTTACAATTTTGAGTATAAAAAATAAAATTCCAGTTTTAAATAAATACAAAAATTGTAGAAGAATAATAGTTAATGATATTATGCACTCATAGTCCTATCAAATGATTCATATATACAAGCGTCTATTTGTAATTCATCGCCAAAATGCATGTTTTCTTTTCTACACAATCTAACTCTTAGTCTCGCTTCGCTAAACAAACATAAAAAGTTTTGATAATTGTTTCTAACTTCTTCTATTTGAACTTTTAACAATTCAGCAATTTTAATCATTTTTCTTACTTTTCTTCTAGTTTTTTTATGACTAAAAATGGTTAAAAATCCTAAGCTTGTTAATCTGTTAACTAGAGTTTGATAACTAATTTGTTTTTCATAGCCTACATGAGAATAATCTACAATTCTTATTTCTTTTCAAAAAGATTTGATTGTTGCATCATTAACATTTTTGATTCCGTTAATTTTGTTTTTAATATATTCTGTGTATTCTTCCATAAAATCATTAACTGCATAATTTAAATTTTTCTTGATTGCATTTTTATTTAATCTATTTTTAGAAACAATTTGAATAGCATCGGTTTTTCTATTTAAAAAATCTCTAATATTGTTTTTGTATCTTTGAATAGATCTAATTGATAAATCAACTTTTTTAGCAATTTGTTAATTACTCAAATTCATTCAGGATGCAATTAATTGATAGTTTTTTTGTTTTTCCGATTTTGTTGGATTAGAACAAAAACTAAATACTAAATTGATAAATAGCTCATATTTTACAATTATAAATGCTAAATTGTTTTGTATCGAAGGTATGACAATCAATTTTAGAATTTATACTTATAAAATTAAAATGATATTTTTTACATATATTGAATTATGTTAATTTATATTTAGCATTTTTATATTGACCAACTTTTTTGATTTTATTTTGTTCTAATAAGACTTTAAGTTTTCTTTCTAATGTTCTTCTTGAAATTGAAACAAGTCGTTCACTTATTTCTTTAGTTGATAAAGGAATTAAGCTAGTAGTTAAAATTTCGATTATTTGATTTTCTAAATAATCTTTTTTATTTACATCTATTAATTTATTGTAGGAAATAATTAAGCAATCTAAAAAATAATCAATAAAAGGCAAATAATCGTTTTGGCTATACTGTCAATTAGTTGAGCTTTTTCTTAAAGTGAAATAATATTGATTTAAATTTTCTTCTATGATTTTTTCAAAACTAATGTATTTATTAATCATAAAATCATTTTGAAATAATAAACAGTTAGTTAATAATCTTGAAATTCTACCATTGCCATCACTAAAAGGGTGAATACATAAAAAATCCAAAATAAATGTTGGAATTAGTATTAAAGGATCAATTATTTTTTCATCATTTCTTTTATTGTATTCATCACATATTTTTGTTATGTAATCATCAACCAAAGAATGATCAACCGGTTCAAATATAACAGTTTTATAGTTGTTTTGGTCAATTGAAAAAATATAATTGTTCATTTCTTTAAACTTTCCTTTTAAATAAGTTTTATTACTATATTTATATAATTGATTATGTAAATTTAAGATGTTTGATTTAGTAATATCTATATTATCAAAATTATTTAAAATAATATTTAATACATCATTATAACCTTGAATTTCTAATTCGTTTTCGCCTGTATATTTTTTATTATTAAAAATAATATTTTCAATTTTGTTTGTAGTTGAAACAATATTTTCAATTTTGTTTGAAGAAGTAACACTTTGAAAAATACTATTTTTAATTAAAGTATTTTTAGTTTGTTCTGGAATATTAGAAAAATCATTTATAAGTCCATTTAACTCATAAATTTGTTTTAATTTTTTGTTAATTTCATTATTAATAATGTTCTTTAAAAATGAATAATCAAAAAACCTTTTTTCTTTTTTAACCATAATAATTAATTATAAACTAAAATATTTTTTACGACATTTTTTAAATTTTGTCGCTAAAGTTAATAATGTTGTCGTAGAAGTCTAAAATGACTTTGAAAAACATTTTCGAGGGGCATTTTTCAAAAAACGTGTTTCTAATTCTTGCCAAACAATAAATAATTTTGTAAATAAATGGTATTAAATACCAAAATGAAGCACTTTTTTATTAATGTTATGTAAAAAACAAACAAATTTGTGGTTTTATATTACGATATATTACATTTTAATTAAACAAATGATTTTATGTTTTTAAAAAAATCCAAATTTTATCAATAAAAAATGTCCTAAAAAAGTCACTTTTTCGAGGGACATTTTCGAGGGATATTTTCGAGGGACATTTTCGAGGGACATTCTTTTGTTAGGTATTATAAGGGCCTATTTATCAATAATTTCCCAATAACCATTTTTACCTTTTCCAACATATTTTATGTTTTTTATTTCTCTAATATATCTTGAAATAGTTTTTTCAGTTAAATTTAAAATTGCTGCAATTTCACTTCTTTTTATTGTAGAATTTTCTTTAACTAAATTGAAAATTCTTTCAATATTTAATTGTTTTTTGTTTGTAATATTTTTATTATCTGTATCTATATTAGAGGTCATTTTTCCAACTTTTAATGTAGCAATTTTTGGAATAGGAATAATCGTTTTAAATATGTTACTATCCAAAAATGTAGGTTTATTATTTGAATAAAGTTTTGTATATTTATAAGTTTTTCTAATTCCTGAACCTAATTCATCAGCTAAACCAATTTCTCTAAAAACTTTTGAAATTGGTGGATTTTTTGGATTTGGTCTAAAGGTTTTAGGATCTAATTCCTCTTTACTGCTTGAACTATTATCATTTTCAACAGTTATATTGTTTTCATCAATGACAATAGTAGCTGTCATATTGCTTGCATATTTTCTATGAGCTAGTGTATTAGAAATGATTTCTCTAAGTATCAAATTACGAGCACTAACACTTACAATACCATCTAAAACAAAAATATCGCTTAAGTGTTTTTCACCAAAATTAATTAATTTCTCATAGCTATCGATTAAATTGGTTGCTACTATTTCTCTATCATCATATCTATCTTTATTTTTAACTCTAAAAATAGCATCTGTACGATATTGAGGAAGAACAGACATAATAGTTTGATCTTTTCCAAAAAGCAAAATAGCTGCTATAGTTATTCCTTCTTTATTGGTTTCAGGATCTTTTAGAATTAAACCTGCGCTTTTAAGCATTTCCTCATCATTTAAGTTTTTTCACAAATGATTTTGATTTCTAATAATTGCCATTTTTCTTGCTTTATTAATAACTTTTTTGTCTAAATCATTAATACTTAAATTAGGATAAACTTTGTTTACAAAACAAATACTTTTTTTCTTTTGAAATAGTTTTCAAACTAATTCATCTTTATTAGTGATATCAACATCACCTTCAAAAGATCTTTCAAAGATTCTACCATTATGTCAACAAACATTTACTCCAACTGGAACATAAATATAAATTATTTTTTTATCATCAAAATCTATTACTTCAGGTTCAATAAATAAAGGAGGATTAATTTTAGTTGAATCATGAATTGAATTCAAGAAGTCATTTACTATTTTTTTAACTTTAGTTTTGTCTACACCTAAAACTTCACCATTGTCTCCAATACCTAAAATTAGATGACCTCCATTTTTATTGCTAAAAGCTGAAACGGTATCAAAAACACTTTTAGATAAGGCATCTTTGCATGTTTTGAATTCTATTTCTTCGCCTTCACCTTGGATTAAAAGTTTTTTCAATTCTTCTTTATTCATAATATCTCCTTATATTAAATATTTTATATTATTTATACAAATCTATCATCTAAAAGAAAATCATATAAACCTATTACTAAAATATTATCTTTAATAAAAGATTTTCCTCTAAAGTTCTTAACAATTAGTGATTGTAAAGTACCTTTTTCAACTTTTTTCTAATGGTCTTATTTTTCTTTCTAATACACCAGGATTATTAATGTCATAACAACATTGAATATAGACTTTAGTTATTGAATTTGTCACTACAAAATCAATTTCTAATTTTTCTATTTTTTGTCTTTAGTTCAAGTTTTCGATTACACCAGCATTAACATCGTAACTTTTTCTGGAAGTGATAGAAAGAAGTGTGTTGAACACTTTTTTTATTTCACTTTTGATTATTTATAAAAGTACTCATAAACATTAATTCATATTTTTATTTAGTTTCGTATTCTGCTAAATTAATATACCTTTCAAAAAAATGATCAAAAGTATAAAAAAATAACAGCGCCTAGAACACCACCAATCGTTCGACCTGTTGTATATATTATACCACAGTCGCTGTTATATGTTCAAAAAAAAGGAGAAATTATGAGTGTATACGGCGAACTAAGGTTAATTGCAAATGAAGGAAGCGAAAACGAAGTTAGAAAGTTTGAAGCTAACTTGGAATGGAGAAAAAATTATTTTGGCAAGAAGGTTTATGACAAGTTAAGCCAAGATACAGTTAGTGAAATTTTAAAAACAAAGATAGTACTCGGTGAAAGCTACTATAAGATTTTGAGAACTGAAAAAGTTGCTTTTGAAGTGTATGTCTGTTTAAGGTTTCTTGGGGCTAAAAAACGCTATGTAAATTTTTATGAATAGTGGCTTTGGCTTCAGAAAACAGTTGCAAGAGCGTAAGTTTTACAGATTAGGCTATTTAAAGTAGAACGCTGTGAATAGAGTTAGCTACTATGACATGAGTCATGTATATGTATAGACTGAATTCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAAGCTGATAAAGAAAAGAAAGCGGAATAAGAACCAAGAACTTTAAGGAAGTTTTGAAGAAATTACCTGCTCTTGCATTAAAAGGGTTCTTATGTCTTCAAGCATATTTAGTGGGCGGTTTGCCTTTAGTAATAGGTATAACTGCTATTAAATATTTCTTTGATAAAAGAAAAGAAAAAGCTTTAGCTAAAAATGAGATAAAGCCGGAAATTGCAAAGAACTTTAAGTTGAGCGATGGTGTTGCTAAAGAGATAACAAAAACGTTTAATGAAGCAATGAAAGAAATTGTTGTTTCAACTAAAGAGGCTGTGATGAGCCAATACCAAAAGGATTTAGCAAGTGGAAAGATTATAGCTGACGCATATGATAATGCGAATATTGAAAAAGCACAAAATGCAGCTATGAATAAAGAAATTGCTAAAGAAACAAAAATTGAAAATCCAAAGTCAAAGGCTAAAAAATAGGAGGAACTATGGAAAAGAAGAAAGGAAAAGGAAAAATTATGCATGAGATTAAAAACAATAAATTTGATGAAATTTTATTAAGTGAAGAATCAAAACAAAAAATAGATCAAGATTTAAAACAAAATGGTATCAAAAGTACCTTAGCTAAAAAATATCTTGAAGAATTAAAAACAATAGGAGAAAAATATGGAATATAGTCAATTTGAAGCAGAACTTAAAAGCGCCTTAACAAATGACCCTACATTAAAACTTTTAAAAGAAATAATCAATAAACCTTTTAATTTTGTTTCACCAACCAACCCATTTGATGTTAAGACTAAAATTAAACAATCATTTTTGAAGCATCAAGAAAGAATTTATGACAAATTTATTCAATATTGTGCTTTAAGTTTAATTACTCAATATGGTTTTAGTTTACAAAGAAGCAATTTTAGATACAATTTGCCTCTTAAAGAGGGACAACACTTAGGAGACATTCCTGAACAAGTAATAATCAATGCATCTTTCATTTTTAGATTGAATAATAATTCAACAAAAGATGAACCAACAGATATGTTTGTTGTTTATTTAAGAAAGAGAGATTGCTATTCACATAAAGCAATGAAAAAAACTTGTGAGAATATCATTAAACAATTAGAAGCCTTAAAAATTATTTACCCAAAAATGAATATTAAGGGTGCTTTATGATTTTTAGATAATGATTATATAAAGAATATTAGATTATTTAATAATTTTTATTATGAACAAGTAGACTTAAGACCTTATTATGGAGCTGATTTCTTTGAAATATTAGATAAAAAAGAAGATTGATTAAAGATAGAAAGTTTTATAAAAAAATTTAAAGAAAACAATCAAAATTATTTTTCGAAAATGCCAGATTTAGATCAATCAGATGAAGCACTTATAGCATTGCTTTATTTGGAAAAGTCAGAATGAACCAAATTAAATTCAAATAAAGAAATTTATAGAAATATTAAAGAAAATATTTTCAATTTAAATGATAAAAATTCTAATTTATTTAAAGCAATTGCAAAAAGGAACAAATCTTATTAATTATGAAAGAATTAATAATTTATTTAAAAAGAATCAAGTGAAAAATTATTAAATAAGACAAGGAGAAAATATGATTAGTAAAAGAAAAGTAGATGAAGATTTAAAACAAAATGGTATCAAAAGTACCTTAGCTAAAAAATATCTTGAAGAATTCAAAAAAATGGAACCAGAAAAATATGAAAAATTTAGAAAAGAAAGCAATAAAAAAATATGCAATAAGCTTTTGCTAAATAGAAAGAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATGAAGTTTATGGCAATTGGAACGATAGTTCCTTTAGCAAGTTTAACGACGAGTTGTTTTGGTCCTATTTTTGAGCCACATCAACCTCCAAATCCAAATCCGAACCCTAATCCAAACCCTAGTGGCGGAGAAAGCACGGATTGGAGCGATGTGGATGGCTATGTACATTATGATGCAGAAAAGAATCCAAAAGATTTTCTAAAGAAGGACAATCAATATTATTTTGCACAAGTATTGCATAATGAGCAAAAACATGATGCCGATGTGTATAGAAATGATAATTGAAAAAAGAACGAGTATATGCCTAAAATCAATGTTCGTTTAGACTTGGATAAAGACTGGTATACTAGCCAACCTGAATTAAACGAAATTAAAGATTTATTCGATGTTGATTATAGCGCGCCGGTGAAGTTGAATAATATGATGAGAAACTTTTTGAATCCTGAATATAAAGATTTAGGTCTTGTGGATTCAAAGAATTATATTAATTCAATGGCTAGTTATTTAGAAGGACCGCAAAAAGATGTATTTGCCTATAAGGATAGTTATCATGGTAATAAATGAGTGTTTGAGGACCAAAAGGAAGTAAGAGAACACTATCACTATTATTTTGATATGGACCTTGATAATTACTATAAACAATTCATCAATGATTTTGCTCTTAAAGGCACAAGCAAGACATTTAAAGATATTGCTATTGATGGTTTATATAAACAAGGAATATGGAATAATTATTTCGACTTAGGAAGCGATTATTCAAAATGGTTTTATTTAGCTTTTACAAGAAGTAATGTTGAAAAATACCATGAAAGCTTTAAGATTTATGAGACTGCAAAACAAAACAAAGATTTCATAATGCAATTGCCTACGGGTGATGATTATAATCTTTGAAATGATTTAAGTAGTTTTGCTTGGATGGCTGGTAAGATGGTTAAGCTAACGTTAGTCAATAACGAAGCTTATAAAAAGTATTTAGAGTTATTGCAAATGTTTGGAACTATTCAAGCTGGAAATAAGAAGTGGTTTGAAATTGAACCAATATATAGAAATTATTTTAAAGAGCACTTGGATAAAGCAACAAATGCATTGCTTAATGTTCTTAAATATGGAGCAGTCTTTGGTGTGGAAATGGAAGAATTAGGCAAAGAGAAAAAAACCTTAGTTAAGATGTTTCCAGTAGCTATTGGTGATAGTCAATTTGCTGATTATGAATATAGCTACGTGTGGGCGTATAGAACACTATATGATGTGCTAATGCCTATGTATGGGGCTAATGATTGAGAAGTGCCATATAGTTTTACTGAATATGATAATCAAAAGTCAGGCAATACACCATATGCTCCAATTTACGAATACATAAACTTTTTTACAAATACTGTTTTTAAAGCTAAAGATAAAAGTTTTAAAGAACTTGAATTAAACACTAAATTTATGAATAAAATGCAAGAGGCTGAATATAAAAAAGAAGCTAAAAGTAAGTTTCATAGCTGAATTGCAATTTGGAATAATAGAGTTAATGGAAGCTTGGATACAAGCACTTTAGATTAAAAATTATGCTAACTTTACTACATAATAATGTGTGCAAAGAACTATTTTTCTAATAGATGTTTTTCTATTAGTTTTTTTGTATTAATTTATTAAGACAAAAAGGAGAAATTAAAAATGAAACCTATGATACAGAAGGATAAGTATTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTATCTTGTATGCTTTATACAGAAAGATTACGATTAAGAAAAAATAGATTCGTTAATAGCAGAAGAAATACAAAATTAACACGTTATGTTCATTTGTATGATGAAGAACACTTGTATATTAGTGAAAAGAATATAACTACTTTGAACTATATATCAGATACTACAAAGCTTGTGCGTAAGCTTGATTGCGGGACAATACATACCACGCAAAATCCTAGTGATTATGCTTCAAGCACGGCAGTTGAACAAATTGCTAAAAAGATTACGGGGAACTGTCAATATTCATTTTTTCTAGGGTTAGTAGGAGATGATATTGAGGCGGTTAAAAAGTTATACAAAACGGCTGAAGCTCCATTACTAGATTCTGAAGTTAAGTTTATTTCAGGAAGAAGAGTGGGGAAAGTTTTAGCAAGTATGAGTAATGCACTTAGATACAAAATTAATCTACATTACAATCCAAAGGAAAAGGAATTATTCTTTGAAAAGGGGGAAGATGAAAACATGAGAAATTAGTCAAGAAAATAAAAAAGGTGATAAATTATATGTAAAAAGCTCTAGAGCCTCAAAAAACGGCTCTAAATGCATTTCTACGCGTCTTAGTGAACCAGATTATGTGGCGTTATACGAAAGATGGGTAAAAAGAATTAAACAAAGTGGCTCTACTCCGTATGTAGAGCTTAAAGATATAATTCGCAACGCTTTAATTAAACAAGAGAAAGAGGAAATAGCTAAAGATTTCAAAAATGATTTATTTTATGCATTAAGAAAAGTATCTTGAGCGTCTTTGACTCCATTTTATCGCAAGATTATAAATGAAATAACAAGCAAAATATTGATTTGGTTTTGCTTGATTTAAAAGTCAATATGTTGTTAAATGCTCTTATAAGCGATAAAGGAATGTTAGATGAACCCTAACATATTTGCTTAAGAGCCAGATATGCAAAGCGTTTGCGTGAAAGATGAATGAATCGCTCTGCATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAATTTAAAATGATTTTTTCACTTTTCTCTTGTAATTGTTTTTTCAATTGCATCTAAAGCAAAATAACCCCAAAAAGTAGAATTATTTTTTATTTCATTATTAATAGTTGTTCTTGAATAACCTAATATTTTTGCTATTTTTGTTATACTATAATTAGTTGCTCAAAGTAAGTATTCAAGAACAGTGCGTTCATTTCATGATAATTTTTTTGCATTTCTTCATGACAATCGCCAAATACTCTTTGGGTGACTTTTTTTATAAAAAAAACTTCACCCATTATAGATTTTATCTAAAATGTGTAAAGTTTCAGATTACAATGTAGCATAAGAAGGCAATACCTTCTTATTTTGCAAATTGTTTTACTATTTCAGTTAAAACATAAATCAAATAAAGATGATTTAATATTGTTTTTACTGGATCAGAACTATTTTGAATATTTAAAAAGTTATTTTCAATTTTTATTCAACTGCAAGACACTTTATTTTTTGGATCAATTAAAAATCATAATCTTTTTCATTCAATTTAAATTCTTCAAAAGTCTTAATTGTGTCAGTTAGTTCATATATTTCTTTACTTGAAGTTTTGAAATGAATTTGATCATTTTGATGAATAAATTTAACTTTATTTTTCTTTTTTAAATAATCATAAATTTCATTTTTACTTCCACATGAAATATATGAATTTTCAGTCATATTTTTATGACTTCCTTCAATTAATAAAAATGTTTCGTTCTTATCATTTGATTCAAATGTTACTTCTTGAATTTTTCAAGTATTATTTTGACCAATTTCACTGTTTAATAGTGCTTTTCTTAAAATTTTAACTTTAGAATTTTCGCCATTTGTAGTTAGATTTTTGCAATAATTAATTCATGGAATATTGTTATTTTTTTCTTCATTTTTGTATTCAAAATTAAGTAATTTAAATAAATAATTTAATGAATTATTAAGATTAATTTTTGCCAAATTTCGACTTTTTTCAATAACTTTTGCCTTTCTTGAAAATGTAAAACTTATTCCGCAAAACAAATTTGTTATAATCAAAATTGCAACAGTAGCCCATTTTAAAATTTCAATCTTATATGACTTTTCAAAAAATGACAACATCAGAACTATTGAAATTGAAGTCAATATAATTAATAAACACAACCCCATTATTATTTTTCATAAGAAAGATTTACTAAGCTTTTTGTTTTCTGAATCTTCTTTATTTAATTGTTCATTTAAATGTTTAGACAAAGGAACATATGAACGAGATAAAAATTGATTAAATGTTTCTTTCATTTTTCTCCTTTATGAATAATTGGAAATTTGAACCATTGATATAAATCAATAAACTAAATATATTTGATCTAACAAATTGTTTACAACTTTGTTAATAAAATTTTGCTTTTTGATTATGTATTTTGGTAAATTTAATTCTAATATTTTTGTATTCCTTTTTATTCAGCCTCTAATTAAATCTCTTTTTAAAAATAATCCAAAATCTTTGTTTTTATCAAAATATTGTTCAAAGTGATTAACTGAAACTTCCATTGCATATGGTCCATAAAATTTTAAGGATTTTATTTTGTCATTTGAATAAGGACTAAAAATATCATTAAATTTTTTATTTTCAAATCCGATATTGTTTTTAAATAATTTGTTTATTCCACTTCTCTTTCCAAATGAGAAAGAAAAGTTTTTGTTACTTGATTCGCTAAATTTTTTAAGTTTAGTTTCAAAAAATATTATTCATTGACTTTTGTCCTTTTCTCTTATACTTGACATTTTGTCAATTGGATTACTTACCCATGAAAATTTAGCTGATGTTATATTTCAATGATTTTTTTGATTATCTATTATTCCTTTTGAATCTGTTTTTTCTTCAATAAATGCTGATTTAGACACTCAAGAACCATCTGGAAAATTGTTCAATCAGTTAAAAGGTATTTCATTAATTAAAATATCATTTATTTTTTTAGTTAATTCTTTTCTTTCTTCTATATTACTTTTTTTCTTTAATTCTCTTGATAAAAAATTCTTTTCTTCTTGTTCACTTACATTTTCTAAATATTCCGACTCAACTATTTTATAGTTAATTTGGTTAAAAATTTGCTTGTAAATATCTTCTTCATTTAGACCTTTGTCAATATTTGTAACAATCGAATTTTGTTTTCTCAAAAATGTAATTAATAAAATTGTTCCTGCAAAAATTGAAGCTAAGAATAATGCTATTTGTAAATATCCTGTTCAGTAAAACAATTTAGAAGAACTTATTAGCAATAAACAAAGTGAGCCTGAAATTCAAAAAATTATTCCTAGAAATATTAAAACAATTGATGTAATTAAATATAAAAAACTTGTCTTAATTTCTTTATTTTTATTGTCTATAATTAATTGTATTTTGTCATTTACAAACTGTTTATTTGTATCTTTAAATTCCGCAAAAGTCTTTGGTACAAAAATCTTAGCCATAAATTCCTTTCCAATTTTATAAATAAATTTTACTAAAAAATAAAATGAAGTATATTACTTCATCTTATTAAAAATTAGTTGAAATATCCAGGTATTGTTAGAAGTGAGAATAATCAGTAAACTGAATATGTATCTTCAAGAATATCTTTACATACAGTTTTAACAATTTTATCTTTATTAATTGTGTTTGAAGGCATATCAATTTCTAAAATTGAACCTCGAGGACGTACTCAACATTTAATGTTTTGATCATCTAAAAGCATACCAAACGATTTTACATAACTTTTATTTTGTTTGCAATGTTCAAGTGTAATCTCCATTGGATAAGGTTGGTAAATATTGTGGCCTTTTAATGAATCTGAACAATAAGCTTTGAATAATTTGTCGAATTCTTGATTTTCATATTTGATATTTTGTTTTAAATTATTAGTTAATCCGCATCTATTCCCAATTGCTAAAGTAAAGTTTTTAGATTTAGGAAGAGAATAGAAGATTCCTTCAAATAATGTTGCATAATCATAAATATGTCTAATTGTTATATTTCCTTTTAAGTCTCTTTCTTCTCTTACTCAATGATAACGAACAGTTTGAATAACTCATGGATTTTCATTTTTGTCCATAACAAAATAAATTTTTCCAGTTGAGTCTACATTAGCATCTGAAGGAATTCAATATGCACCAGGAATATGATCTACTCAGTCTAAACAATCTCTATTTTTATCATAGCCTTGTTTAAATTTTTCTAATAATTGTGCTTGTTCTGGGTCATTTGGATCGATTGCTTTGTATGATAATTTTTCAAAAGCTTTGTCAAATAATTTTTCTTTATCAAGAACGTGTTTGAGTTCTTTAAAAAGTTTTTTCCTTTTTCCTAAAAATACTGATAATACAATTGAACCAATTCCTGTTACTATTGCAGAAATAATTAATCCAGCAAATATTGCTGTATTTTTTGAATTAGTTTTTATGAAGAAAAAGAAAGATAATATTAATCCAATTCCACCTAAAACTAATAGTACTATTGAAACAATTAGTAATATTTTTAATTTTGACAAAATATTCTTTTTATTTTTAATAACTGAATCAAAACTGTTTGAAACTATTTCTTTAAATGATTGTAAGTTTTCATTCACAAAATCTAAATAACTCTTTGGTTTAGTTGTAACTCCCATAGTTTCTCCTTACTAAAATAATAAAAATTATATCATATATAAAGCTTGATTATTTTGATTAAATTGTCTTGTTTTTTGTTGCTGCAAGCATAGAGATTTGTGAATAAATAATATATAAATCATAAGTAATGTCTTCCATTATTTCATCAATAATGATTTCTTTATTAAATGTTTTTGGAAAATCTACAACTAAGAAATTGTTAATAGGTTGTAATCAAGATTTAATACTTTCACTATCTAATTGCATACCAAAAGTTTTAATAATTCTGAATTTAGTTTTGCAACTTTAATCATTTTTTTCATAGAATAATAATTTACTATTTGAATAGGGGACTTAAATAAATAATTAAATTCTAGAAATTCTAATTCCATATTTATTGGTGTGTCAAAATTAAGTCCATATCTCTCGCTTAAAACAACTTTTCTTTTTTATTTTAATTTTAAAATTGCAACTGTTTCTACATGGTGTGTTTGACAAAACATATCACATGGTTTTAAAAATTCTAATTTGTATTTTTTTGATAATGTTAATAAATCTATATCTCTGCAAAGTGTGTGAGGATTACAACTTATGTAACCAATTTGTTTTGGTTCAATTTTTAATATTTTTTCTAAAACTTCTTTCGATACTCCTGCTCTTGGAGGATCTATTATTATTGTGTCTATTTTTTCTTTAATGTTATCAATAGTTTTTAAAACATCGCCTTCATAAAATTCTGTATTATTTATGTTATTTAATTTTGCATTTTGCTTAGCATTTATAACTGCTTCTTTTACTATTTCAAGTCCATAAACTTTTTTAACTTTTTCTGCAATTTTTAAACTTATTGTTCCGATTCCAGAATATGCATCAACTACATTCATAGTTTTATCAAGGTGCAAATTATTAACTAGCAAGTTATATAAATTATTGGTTTGATACGAGTTAACTTGAAAGAAACTATTGTAATCAATATTAAAAGTTAAATTGCTAATTTTGTCTTGTATGTTTTTAGCTTCTGAATAATAAGTATAAGTTGTTTTTTTAACTTTTTCTTCTTTTAATATAACTTTTAAATTTTTAAAATTTGAGGTTAAATCTTTTAATAAATCTTCAGATAATTCAAATGGTTTGTCTTGAACAAAAGCTAACATCATTTGATCATATGATTCTGAATATCTAACAACAATTTGTTTTAAAAATTGTCCGTTTTCTATAAAACATTTGTAATTGTTAATATTTTTGTTTAAATACATAAGAAGCTTATTTATAGACTCATTCGCCAAATCATAAGAATCTTGCTCAACTAATTTATGTGTATTTTTTTCATATAAACCAAATTTGATTTTTCCTTTTGATGATTCAACAAAAACTGTAATTTTGTTTCTATAATTTCATTGTTTTTCACACGGCAAAATTTTTTGTGTTTCATTAAAATGGATATTTCTTTGAAATAAATATTTAACAACATTTTCTTTAAATTCTAATTGATCTGCATAGTTTAAAATTGCTAATGGAGTTGAACCACTTAGCATTAATTTTTGATTTAAAACTTTTATTCTTTTTGGACTATCGTTATATCTTTTTACAACTATAGCAAAAGCAAATTTTGTGTTCGATTTTTTAATTTGTATATCTGCTTTTTCTTGTGGCAATAAATTCTCAACAAAAATAGAATAATCATCTGCTTTAACTAAGCCTAATGCTTCATAAGTAAAATTTATTGCTTCCACTCCTTTTATTATTTGGCCTAATTCATATTTCATATTTCTAATTTTATATTATTTCCACAATAAAAAAATAACTTTAACAAAATGTTAAAGCTATTAAAAATAAATTCTAAGATTTTTCTTCAGGTCTACTGAATACATAACGACCTTTTTCTGTAACAAGAACATCATCTTCATTTCTTGCTCCGCCTAAACCTTCAATATAAATACCAGGTTCAACAGTAATTATCATTCCAGGTTCTAAAATATAGTCACTTGTTGATCTAACATTAGGTAATTCATGTACATCAATTCCAAGTCCATGACCAGTTGAGTGAACAAAGTATTTACCATATCCTTTTTTAGTTATGTAATCACGACAAATTTTATCAATTTCACTTGCTTTAATTCCAGGTCTTACTGCATCACGACCAGCTTTTGCAGCTTCCATAACTATTTGTAAAATTTCTAATTGTTTAGGATCATTCGCTTTTTTGTCTCCACCTAAAATACATGTTCTAGTAATATCTGCACTGTATCCTTTGTAAAGAGCACCAAAGTCGATTTTAAGAAGATCCCCTTCTTTTAATTTTCTATCTGTTGGATGGTGGTGAGGTTCAGCTGATGAAGGCCCTGTAGCAACTATTTCATCAAAACTTTCTTTATCTGATCCATGTTTTTTCATTAAATAATTTAAGTATGCAGCTACTTCTTTTTCTGTCATTCCAGGTTGTACTCATGTCATTAATTCTTTATATGCAGCTAATGAAATATCAATAGTTTTTTGCATAATTTCATTTTCTTCTGCTGATTTAAGAATTCTTAATTCTTGTCCTAAAACTCATTCGATTTTTTTAGGTTTTATAAGATTTTTTAGATTTTCATATACTTGGTAATTTAAGTAATCTTTTTCTAAACCAACTGTTTTATAGGCTTTTTTGTCAAAAAATTCTTTTAATGTACCAGCTTGTAATAAAATAACTTCTACATTTTTTGCGTTATTTCTTGCATATTCAATATATCTACCATCAACGAATAAATAAGCTTTATTTTTTTCAATTACTATATATCCATCTGATGTTTGTACTTTCGAATATCATAGTCTTGTTTGAGGCGCTTCAGAAACTAAAGCATCAATTTTCTTTTCTTTAAATAATTTATCTAAATATTTACGATTCATAACTTCTCCTTTTTTGTTAATTTTATAACAATTTATTCATATCCAAATTTTTTAAGAGCTTTACCATCATTAATTCATTTGTTTGCTATTTTAACTTTTAAATTTAATTCCACTTTTGAATCAAATTGACTCATCATTTTCTTTCTTGCGTTGGTTCCTATTTGTTTTATCATTTGACCATTTTTGCCAATTACCATTCCTTTTTGAGAAGCTTTTTTGACATAAATGATGGCATTTATTTTAATATCATCTTCTTCAATAAAATCAATAACTTCTACAGCAATTGAGTGTGGCAACTCTTCTCTTAAAAGATTAATCGCACTTTCTCTTATTATCTCTTTTGCTAAAAATCTCATTGATTTATCTGTTATATAGTCTTCATCATAAAATGGCTCACCTTCATAGGCATATTGCTTAATAACTTTAATTAATTCTTCAATTGAGTTTTCATTATTTCTTGAAACAGACAATATTTTGTCAAAACTGTATTTGCTTAATTCTTCAATTTTTTCTTGTATTTCTTCTGGTTTTTTGGCTAAATCAATTTTAGAAATGACTGCAATTTTATTCTTGGTATTTTTGATCTTATCTAAAATTAAAATATCTCCTTGTCCAACTTTTTCATTAACAGGAGAAAGAAATATTAAACAATCAACATCTTTTGTAGATTCAAAAGCACTTTTATTTAAGGCTTCACCTAATAAATTTAATGGTTTGTGGATACCTGGTGTATCAACAAAAACTAATTGAAAATCATCTTCGTTATAAACGCCGACAATTTGATCTCTTGTTGTTTGCGGAACATTAGTTACAATTGCAACATCATATTTAACAATTTGATTTATTAATGATGATTTTCCTACATTAGGTCTGCCGATAATACTAGCTAAACATACTTTCATTATTTAATTTGCTCTGTTCTAATAGGAAATGGGACCATTTCAATTACTTTATTTACTCTACATTTGTCGCCATTTTTATTGTATTGGTAAATTAAACCATCATCGGGCATAAATTCTGTCATTACTTGACGACATCCTGCACATGGAGAAATAAAATCATCTCCAGAAGAAATAATGTGAATTTCTTTAAATGTTCCAACTTTTGCACCATGAGCAACTGATCCGAAAAGAGCACATCTTTCTGCACATAAGCCAGATGGAAAAGCAGCATTTTCAACATTTACCCCAGGTCATTCTCTACCCTCAGCATCAATTGCAATAGCAGCAACCTTAAATTTAGATCAAGGACAATATGAATACTTCAATAAAGATTTTAATTTTTTAACGTCCATAGATTATTCCTCTCTACTTATTTTTAATGGTTCAAATATTAAATCAACAAGTTTATTCATTTCAGCTCTTTCGACTTCTGTTTCATGATCATATCCCATCAAGTGAATTAATCCATGAGCAAATAAATAACAATATTCTCTACGAATTGAATGAACAAACTCTTGAGCTTGTGCTTCAACTTTTTCATGGCTTATTACTAATTCACCTAAGGGATAAATTGGCATATTTTCAAATAATTCTCAATCTTCTAAACCAAAAGACAAGATATCTGTTGGATAATCTTTGTTTCTATATTCTTTATTCAGTTTTTGGATTTCATCATTATTTGTAATAGTTACATCAACTGAAACAGTTTGATTAAGCCCAAATTCTTTAGCTAAATTTTCTAATATTTGCTTGTATTCTTTTTCAAACAAAAATTTATTTTTAGTCTTGTTTACAACATTTAATTCAACCATAATTCTAATTATATTATTAATTATGAATTTAATATTAAATTTAATAGAGTGGCTCGATCCACAAATATCGTGGCATCTAAAGTTGTATTAGGTAAAAGTTTCAAACTATTAGGCAAATTATAGAAGGTTACTTTAATCGTTTGATTTTCAATTTTTAAGTTGCGGATTTTTAAATTGAAAATTTGGTCATTTACATTAATTATTATTTTTTGATTTTTCTTTAATAAATAAGCAGTCTTATCATTTGCTAACAAAGTTAAGTCTTTTTTGTCATTAACTTGTAACTTAACTCTTATTGTTTTGTCAACATGATATAAACAAATAAAAACAATTAAAGTTACTGTTGCAATAGCTAACAATATTAACAATATTCACACAGTTTTAGAAACAGTTGTTAATTTTTTCAAAGCACACCTCCTTTGTTTTAAATAAATAGTTCCTATTGTGACTTATTTCAATAAATAATGCTTCATCTTGATATTTCTTTATAGCCTTTTTCAATAATTCACAATTACTTGGATTAATATTTTCAAAGGCTTCATCAAGCATGATTAAGTCATATTTTCTTACAAATAATTTTAGTAATAATAAAAGTTGTTTTTGTCCACTTGACAAATTGCTTCCATTACTAATTATGCTGCCATTTAAATTAAAATTCATTTCTTCAAATATTTTGTATAGATCATATTTTTGAATATTATTATTCAATTCATTTTTATAATTTTCATTGTTACTTGTAATATATTCGAGAAAAGAAACATCTGGTAAATGGCTGGAAGAATTTATCAATATATTTTTACTTTGATAATTAATCAAATTTATTGAATCAAAATCAATATCATTAATCTTCATTTTTCCTAAATAACCTTTAAAACAGCCGTTTATAAAACTTAGCATAGTGGTTTTACCACTACCATTTTTACCATTTAATTGAATATTTTGGTCAATCGTAAATGAAGGAATATTTAAAATATTTTTTCCGTTTTCGTAATAAAAATTAACATTTTCTAATTCAATTTTTTTAATTTTATCGATGAATAAACCAGTATCTAAATTGCTTTTTTCGTCTAAATTTAAAACAAAATTTACTAAATTAACATTTCTTTTGATCATTGTTTGAGAAAGTAATAATGAGCTCATATTTAGCATTGGATTAACAAAGAAATTTATTCCATTTAAGAACATCATTAGTGAACCAGTTGATAACTTATTTTTTAAAATAAAGAAGCTAGAAGCAAAAACAATAATGGTTGAAAAGTTGCCAATAAAAAGATCATTTCAAAAGTTTTTAATATCATCTTTAGAAGCAAATGAATATTCTTTTTTCTTAAATTCCAACATGTTTTTAAATTGGTTTTTAGAAATAAATCTTGAATAATCTACATTATCCATTTCTTTTCAGGAATATATAAAATCAATATCTGATTTCATGCTGTTTAAATTTTTCTCCAAGAGATCTGGATAGTTTTTTTCTATTCAAAAATGATAAATAACATTACCTAATAAAACAACAATAGCAATACCAAACATAATTAAAAATAGAACATAATTAATTCAAATCAATAAGATTGTTGAACCTATAAAAGAAAATATTTCACTTAAAAAAGTAAAGACAAAATTAGATTGAAATTCACATATTTCTTTTATATAAGTAAGTCTTTTAAAGTAATCATTGCTTGTTAATTTAGATAAATAATTTGATGGCACTCTTTTTATTTTGTCAAAAAATGCTTTTGTTAAAGTAAACTCTAAATCGTTTTTTATTTTTTTAATAACATGATTTTTAAACAACCGATTAATAAACATCAGAATATTAATTCATAGGAAAAATACAAAAATAATAATCAAATTATTTTTAAGATAATTAGGCAATATAAAATCAAAAACTATTTTTATAAAAAATGTTGATCCAAATGATAAAAACAATCCTAAAAGTGCTGAAATGAATAAAGGATAAGTATATTTTTTAAATGAAAATAATGTGCTTATTTTATTATCAAGTGAATAGTTTTTATTTTTATTTTTTGATTTTTCTTCTTTTGGATCAAAAAATAGTATCACATTGGCAAATTCCTTAGCCAAGTCTTTCTTTTTAACTTTCATCTTGCGACCATAAGCTGGATCTTGAACAATAAAATAATTGTCCTTCATTTTTTCTAAAATTACATAATGGCTATATCCTTTTCTATTTAATAATAAAGCTATAGGCATATTTGAATTGTCCAAAGATTCAAGAGATTCAAAATCACCTGTATAACTATTTAAATCGATATCGTGTTCAGAAGCTAATTTTTTAAGTGTTGAAATATTTACTCCTTCTGAAGAGTAAGTTGCCTTATTTTTAAAATAATTAATATCAATGTAGTCATCATTATGTTTTGCCACAAAATACTGCAAAACATATAAACCACAGTCTTTTATATCTTCTTGTTTTTTTATTCTCATAGCACTTTAAACTTTCTTTTTTTTCTTTAGCAAACACAAAAAATGGAAAAATTTAGGAAAAAAGATAAATTTTTACCAAACTTTTCCAGAATTTTGCTTTATCATTTAAAAATAGACACTAAGGAGGCTAATATGGCTAAAAAAAATATTATTGCTCTTGCTAGCGACCATGGTGGTTGCGAATTAAAAAATGAAATGCGCGATTATGTAAAAAGTTTAGGTTATGAAGTTGTTGACTTAGGACCTGCGGATGGCTCAAAAAGTATTTCATATGCTGAACAAGGGCATAACCTAGCTAAATATATTGATGAACATAAAAACACAATTGGTTTAGGTTTTTGTGGTACAGGTTTAGGTATTTCATATGCCTTAAACCGTCATAAACACATTCGCGCAGCTAGAGTAGTTACAGTTGAAGATGCAAGGCTTGCTAAATTACATAATAATGCTAATGTTTTAGTTATGGGTGGTAGATATGTACCTTTTGCTGAAGCACAAGCTATGTTTAACGAATACATTAAAACTGAATATGAAGGTGGCAGACACCAAGCAAGAATCGATCAAATCGATGAATTCTAATAATTTTTGTGGACTTAGTAAGTCCACTTTATTTTTTATTATTCAGCTTTCTATATAATAATAGATATGGAAAAAGCACTTTTATATGAAATCAAAAAATCAAAGTTTTATTCTTTAGCTTTTAATGTGACTAGTAAGGAACAAATAAAAGAAGTAGAAGCTAATTTAAGAAAAGAATACAAGAAAGCAACTCATATTTGTCATGGTTATAGCTTTTTAAATAATGGAGTGATCAATGCAGGCTTTAGCGATGATGGCGAACCTAATGGTACAGCCGGCAGACCAATTAGAGATCTTTTAATTATGCGCAAAATTAATAATGTTGTTGTTTTTGTAATTCGCTATTTTGGTGGAATTAAGTTGGGAGCCAACGGTTTAGCTAGAGCTTATATTAAAAGTGCAAACTTAGCTATAGAAGAATATATAAAGGAGGGAGCCTAAAATGATTGCTTTAATTGGTCAAATTGCCGTGGGAAAGAGTACTTTTTTAGAAAATTTTAAGAAATTAGGTCTTAAAACTTTTAATTGTGACGAGTTTATTAATCGTGAATATCAAAAAAATGGCAAAATTTACCACAAAATTAATCAAGAATTAGGTGATTTTTTAAATGATAAAAATGGTATAAGTAAGGAAAAAATTAAACTTTGAATTGACCAAAAAGCACATAATTTGGGAGAATTGGAAAAAATCATTTATCCTTTAATTTTTGAAGAAATAAAAGATGGAAATTTTTTCATTGTTGAGATACCAAATTTATTGCCAAAAAATTTTAAATTTATTTCTTTGTTTTCTGCAGTTTTGTGCTTAGAAACTAGCCAAAAAAATAGGTTAAAAAATTTTAAAAAACGTAATGTGGATAAAACTAAAATTAAGGCAATAGATGAAAAAAACAACCCTTTTTCGATAAAAAATCAACTTTTTGGGTCTATACCAATTGTGGATTTCTATGGGCATAACTTCACTAATAATGAAAATATTTTAGATTTTTTAAAACTTCTATTTTTTATCTCATAAAATAAGAGCAAACAAGGTCAAAAATGACCAAAAAAATAGGAGGTAAAAAATGCTAAAAAATATTCCTTACGCATTCTTCAGAATCAAACATTCTTCATCAGTTAGTGGAGAAGATTTAAAAAACTTACGCAAGTTTTACGCTCCTATTTTAGGTAGTGATGCAATTTTGCTTTATGAATACCTTAGAGATCTATCATTTTCTCAATCTCCAGAAACTGGTTTTTATGACTATGATAGCCTTACTTATCTATTAAATATGGATGTAAATAAACTAAATCAAGCACGTATAAAATTAGAATCTGTATCTTTAATTTCTACTTTTATAGATGAGTTTAATCGCAAAACACTTTTTGTTATTGAAAAACCACTTGATAATACAGGTTTCAAAAAGAATTTAATTCTAGCAAACAAACTCATTAGTATTATTGGTAGCAAAAACTTTGAAAGATTAGTTGGACGTGAAAGAAATATTTATTTAAGTAAAGCTTCAAATCTTTTGGATGCTTCAGCTTCTTTTGAAGAAGTTTTTCCTGAACCAAGTGAAGAATTAACTATTACTTTTTCACATGAGGTTGACTCAAGTATGATTAAAACTCAAGAAATAGTTGAAGAAATTCAAACCAAATTGTCACTTAATACTTTTGATTATCCTAATCCTTATGAAGCAATACTTAAAACTGATTCAAGATACTTCTATTCTCAAATTAGTCACAAAATACCACGTCAAAGTGTTTTAGATTTAATTAAAGAAAGTAGAGCAGCTGGCTTAGGTGATCCTTGCTTAAACTTAGTATTCTTTTATTCAAATGAAATAAATAATTGCATAAATACCAATTATGTGGGTAAAATTATTAAGGATTTAATTAAAAAAGAAATCTTCTCATTTGAAGCAATTGAAAATTATTTAGATGACATACTTAAAGTTAAAAACAGAATTGCAGTTTCAAAAATTAATTTATATAAAGCTACCTATTTAGAATCACTTCAAAGAGAAGACAACAACAATATTTAATGGAGTAATTATTATGGCAGCAAAAGAAGAAATTAAAAATGAGTTATTTTTTAATTCCAAGGCTGAATTTGAAAACAAGGCACTTGAAATAATGCATGGCCATCCGCAACTAGAAGCATTAATTCAAGCTAACAATATTACAAATGAAGAAATTTTGGAAAACTTATTTACATTTATTAATATTAAAGAATCATTGGATAATTCTCAAATTTATCCATGGATTTTTTCTATTTCAAGAAAAAATGAAAAATTAGTTTTTAAAAAGATTGCTGCAAAAAATGAATACAAATCAGTTGTTACACGACACATCAATTTATGATTAACTCAAATTTGCGAACCTAATCCAGAAGCTAGACTTGATCGTATTCGCCTCTCTTCTGCTCAAAGAAAAGAAATGTTTGAATATATTAATCATCTAAAAGTTGCAATAGCTAAAAAGAAACAAAAAGGACTAAAAGGCATTTATGTCTATGGAGCTAACAACACTGGCAAAACTTACATTGCTAGTGCAATAGCCAACGAATTTGCAGCTGAAGGTATTTCATCAGTTTATTTAACAACCAGTGCTTTATATTCATTCTTAATTTCAAACATGGGTAGCAATGCTACAAATGCTAATGCAGTTATTGACATAATTAACAAACTCAAAAAAGTTAATGCATTAATTATTGACGAAATTGGTTTAGAAAAAAATAACTACTGATTCAGACTCCAAGTATTAATGGAGATAATCGAAAGCAGAATCGCAAATAATAAAATTACATTTTTCTTCAGTGGCATGAACAAAAAAGAATTTGAATCTTACTACCATGATCAACTTAAAAGAGAGCCTTACAAAGTTAAAACTTTTACTTCTCGAATTCTTAATAACACACTTGAATTTTGCATTGATGAAATTGAAAAATAATAAAAATTAAAAAAGACCATTGTGGTCTTTGCTACATTGTAATCTGAAACTTTACACATTTTAGATAAAATCTATAATGGGTGAAGTTTTTTTATAAAAAAAGTCACCCAAGGAGTATTTGGCGACTGTCATGAAGAAATGCAAAAAAAATTATCATGAAATGAACGCATTATTCTTGAATACTTACTTTGAACAACTAATTATAGTATAACAAAAATATTAGGTTATTCAAGAACAACTATTAATAATGAAATAAAAAATAATTCTACTTTTTGGGGTTATTTTGCTTTATATGCAATTGAAAAAACAATTACAAGAGAAAAGTGAAAAAATCATTTTAAATTTTTAAACAATTTAAATAAATATAAAGAATTTTCTTCTTTATTTAAAAAAGAATATGATGGCAAAATGTGGACTGTCAAATTAACAATTGAAAAAATAAAAAATCTTTATCCTAATATTGATGTTCCTTCTTTTAAAACAGTCTACAATTGAATAAAAAGTGGTTATTGAATTTTGAACTGAAAAGGTTTATTAAGAAAAGTCTACAAAAAAGATGGAAAAAGAGAGAAACAAAGCGCTGCTAGAAGACTGGTTGGTGCACGTTATGTAAGACCTTTTTGATCTAGACCGACAAACATAAATGAAAGAAAAGAATTTGGTCATTGGGAAATAGACCTTATTGTAGGCAAGAGTAAAGGCACACATTGTCATTTACTTAGTTTTACCGAAAGAGTCTCTAGATATGGGTTTTTGGTAAAAATTCCTAATAAAAATCAATGAAATTTAAATTTGTATTTATGGAATCTTATTTGAAAACATAAATTAAATGTTAAATCTATAACTCAGGATAATGGATTAGAATTTTCAACATTATTTTTCATTGGATATAAACTAAAAATTTTTATATATAAAGCTGATCCATATGCTTCTTTTCAAATGGACTCAAATGAAAATTTCAATGGTTTAGTTAGAAGATTTTTTAAGAAAAAAACTAATTTTGATGATATTAATGAAGAAGAAACTCTAAAAGTACAAAATGAAATAAACAATCGTCCAAGAGAAATTTTTGGATATTTATCTGCTTTGGAAATGTACGAAAAATTGAATGCAGAAAATCCTTGAAATTCAACAGGTATTGACAAAGTATTATATGGAAAACAAAAGAGAAACTAAGAAAGTAACAAAAATAGAAATTTGTTTTTCAAAAAAATATGAAAATTAAAATAATATTTTAGAAAGAGCAACAAGTCGCCAAATATTTTAGGGAGGACTAATGCCCTCACTTTTATTTGTCCTGACGGACAAAAAATAATAACAATATTAATAATTAAAAAGGAATATCGACATGCGATATTCCAAATTTCTAGGTGTAAACTTACAGATTACAATCTAGGAAAGACCATTGTGGTCTTTTTTACATACATTTTAAATTTATGTAACATAAATTATTTTTAAAATTTAACTAAGGAAACTATTTCAATATCATTATCTTTAGTGATTAATTGTCTACCATTAAGATCTACTAATTCAAGAAGTAAAATAACTTTTTTAACAATTGCACCTTGTTCTTCTAATAGTTTTATAATAGCTTTAGTTGTTCCACCCGTTGCTAAAACATCATCAATAATTGCAACTGTTTGACCTTTTTTAATAAACCCTTTTTGAATTTGTAAAACATTATTTCCATATTCCAAATCGTAACTTTTTGAAATAACATCACCAGGTAATTTTCCTGGTTTTCTAACCATAATAAATGGTTTCTTCAAGAATGCTGCAGTAGGCGTGCCAAATAAAAATCCTCTCGCATCTGGTCCAACAATAACATCAACATCTTGAGCTAATTTTGCCATTTCTGTGATTGTATAATTTAATGCTTTACCATTTGCCAATAATGGTGAAATATCTTTAAACATTATGCCTGTTTTAGGAAAATTTTCAATATCTCGAATATATTTTTTTAAATCCATAATTTTACCTATTTGCCTTTTTTATAGTCTTATAAAGATACTGGAACATCTTCAAAGAATTCTAAAATATCATCTTCCTTGATATCATCAAACTTCTTAATGTGAGTACCAAAATCTTTACCTTTCTCAACTTCTTTTGAATCATTTAATTCACGTTTAAGTGTTTCAATTACACCATCATGAATCATTTTTCTGCCTCTAAAAACTTTAACTTTACAATTAGCTTTTACAACGCCTTCATCCATCATACATCCAGCAATTGCTCCAACTTTTGAATAGAAGAAAACTTTGATAATATGAGCTGATCCGATTTTCTTTTCTTCATAAACAACTGCTTTTTGACCCTTAAGGTGAGCTTCCATATCTTCAATAATTTTGTAAATAACAGAATAATTAACAATTGTAATTCCCGAATGTTTTGCACTTTGCTTAATAGCTGCAGATGGCTTAACATTGAAAGTTATAATCCTTGCATCTGAGGCTTGAGCTAATAATAAGTCAGCATTTGTTATTTGTCCCGCTTGAGCGCCAATAACTTTAATCATTGCTTCATCATTACACATGCCATCTATTTTGCCTTTGATAGCTTCAGCTGTTCCTTGAACATCTGATCTAATGATTACATTAAATACCTTTTTGCCAATTTCAATTTCTGAAGTTTGGCTCTTGTCATATAAAGCTCTGTTCTTATCGATTTGAGCTTTTTCTTCAGCTAATTTTTTAGCAAATTTTTCATCTTGAAAACCAATAAATCTATCACCTGCAAGTGGTGAATTATTTAATCCAGTAACAACAACTGGTGTTCCAGGGTGAGCATGTTCCATTTGTTTTCCAGTAGCATCTTTTAATGAACGCACACGCCCATAACTTGAGCCAGCTACAATAAAGTCTCCTTTATATAAGCTACCGTTTTCAACAATTAAAGTTGAAACAACACCAACACCTTTATCTATTTTACTTTCAATAACAGTACCAACTGGATATCTTGAACGGTTGGCTTTTAAATCCAATAATTGAGCAAGTAAACTAATTGCTTCAAATAAATCTTCAATACCTTGACCTTTTAAAGCAGAACCAGTTACCATTTGAACGTCACCACCATATTCTTCAACAATAACGTTGCATTCAGCTAATTCTCTTTTAATTCTTTCAAGATCCTTATTTGGTTTATCTATTTTATTTACAAAAACAACGATAGGAACATCAGCTGCTTTTGCGTGGTCAATAGCTTCTCTAGTTTGTGGCATAACACCATCATCAGCAGCTACTACTAAAATAATAATGTCTGTAATTTTAGCTCCACGTGAACGCATTTCAGTAAATGCTTCATGTCCAGGAGTGTCAATAAATGTAATTTTGTTGCCTTTGTAAACTACTTGGTAAGCACCAGTATGTTGAGTAATACCACTACTTTCAGTTTCAGCAACTTTAGTTTTTCTAATAAAGTCAATTAATGTAGTTTTTCCGTGATCAACATGACCCATAACTGTAATAATTGGAGGTCTTTTTACTAATGATTTTTCATCATCTTCAAATTTAACTGCATGCAAAAAATTTGCAGCATCAATGTTTTGTTCTTTTCTAAAGTCAAGTCCTCTTTCCATACATACTTCAGCAATTTCTTCTTCATCAAGTACGTAGTTAATTTGATAAAGCTTTCCTTTCATCAAAAATCACTTGATCAAATCATTGGCATTCATACCAATTTTTTGTGCAAATTCTCCTAATGGCATTTTGTTTGTAAAAATGAAAACACCATTCTTAACTTCAGTTTTAATATCTGAAAGTTGGTTCTTGACTTCATCAACGTTACTTATTCTGTTCTTTCTAGGCATTTTGCGTCCTCCAATTCTTTTTCTATTTTGTCATATACTTCACGATCAACAGCAGTTCTAAAAGCACGATTAAGTCCTTTATGCTTTTTTAAGTTTTCTCAATTCTCTCTTGTAGGTATAAAGTAACAACCTCGTCCCTTTTTGACTCTATTAATATCTAGTGTAATTATATTGTTTTTTTTATCAAAATCAAATCTAACCAATTCTTCAACTGGTTTTATAAGACCTGTGATGATGCACTTCCGAGTAAAATTGGAATTAATTTTTGTTAAATTATTTGTTTTCTTCTTCATCAAAATCAATGTCACTTAAATCAAAATCGTCAATTCCAAAATTCTTTAAATCATCATCAACAACAAAGTCTTTTGCGACTTCTTTAGCTTTTTTATAGTCAGATGCGGAAATTTTGTTATCTTGAGCAACTGGTTTTGTTACTTCAACATTTTTAATGTTTTCTACTTCATTTTCAACTGCATATTCGCTTTCAATTTTCTTAACTAATTCTTCGAAGTTTGAGTCATCTTCTTGTAATGAATCCAATCTGTCTTGTTCATGTAATTCGAAAGCAGCAATATCTTGATCAAAGTTTGAAACATCGATTGAATCAAATGATTGAGCAAAAATGTTGTTAGCAGGTTTCTTATTAAATCTTCTATTATTGTTAGATTTTTGATATCTAATTTTAGGTCCTTCTTTTTTAATGGTTGCACTAATAGTAGGAACTAATGTTTCATCAAAAATTATTCCTTGAGCTTTAGCATCAGTTATTGAAATAATATCTAATTTAACTTTTGTTAAACTTGAAGCTAAATAAGCATTAACCCCTCTTTTACCAATAGCAGGTGTTAAGTGTTCAGGAACAACAATAACTAAATATTTGTTTTCTCTTTCATCTTTTAAAATAACATCAACAGCTTGAATTGGACTAATTGAGTTAGCAACATATTTTTTGATATCTTCATCATATAGAATAACATCAATTTTTTCACCATCTAATTTTTGACTTAATAAATCAATTCTTGATCCATTTTCACCAATAATTGAACCTCTAACATCAATATCAGGATTTGCGCCTTCTGCTAATTTAACTGCAACTTTAACTCTTTCTCCTGGTTGACGAACTACATCAACAACTTCAATTAAACCCTCAGCAATTTCTGGAATATTGTTTTGTAAAATATTTCAAACAATTTTAGGTGAGTCTAATGAAACTTCAATTTGACTCAATTTTGTTTCTTCTGAAACTTTTTCAATAACAACATCAAAATATTGACCTGGGTTTGCTTTTCTTTTTGTACTAATTAATCCTGCTGGTAAATATGCATTAACATTACTGTTCACTATTTGAACATTTCATGAACCATTTGAATTTTTTGAATTCAATACAACTTTAACAGTTTGTCCAATTTTGCTTGAGTATTGTTCAAAAACCATTTTCTTTTGAAGAGTTTTAATAGCTTGTGCAATTGATGAGTTGATAATTTTTAATGATTTTTTAGCTTCAGGATTTTTGCTTTTGTCTAATACTCCTAAGTCAATTTCTCATTTAATTTCATCATCTTCTTTAACTTTAGGCATTCTTACTTTTGCTTCTTTATATCCAACAAAACTTATTTGATTTTCAAGATCAAGCAATAATTCTTCATCTTTAGTTACACATGTTTTGTTGTTAAAAATGTGAACTTCTTGTTTTTCTTTGTCTAATTCAAAAACTATTTCAGCATTTGGATCTAAGATTTTGTTAATAATTCGTGTAGTTTCTTCTGAAAAAATTTTGATAACTTCTTCAATATGAAGTTTTTCTTTTTCAAAATAACCTATCAAAATTTGATATCAAGCTTTACTTGCATTAAAAGTTTCTTGTGCTTCTGTTTTACTTTTTCTTGGCATTTTTTCTCCTTAATTAAACTTTATATATTTTTCTACTTTTACTATGTTATTTTTGTCGATAAATACTTTTCTAAATTGGCCTTTGTTGTTTCAACTTATTTGAATTTTGCCTTCTAAAACTTCTAATAATTTACCATTAAATTTGTCTTGTTTTTCAATACTTTTATTTAATTTTATAGTTAACATTTCTCCAATGTGTTTTCCTAATTCATCTGTTTTATAGGTTAATTCGACACCTGGAGAATGTATTGATAATGAATCAATATCCAAAGATGGATAATTAGAATCTATAAATTCATTAAGTTGTTTTGATATTTCTTCAACCTTATTTAAGTCATGATAATTGACACTAATGTCTAACAATTTTAAGTCATCTTCTTCAATAATTTTTGCTTCTAAAATTTGACTTCCAAATTGTTTTTCAAGTATTTCTTTTCAATTCATGAAACCTCCAACAAAAATAACAAAGAGTTGCAAGTTGCAACTCTGTAATATTCACTTGATGGTTTTATTATATCACAAGATTTTTAAGTCAACAAAAAAACTTGCACTCGTGCAAGTCTTAAAATTATTTTTCTTGGTTGTCGTTGCCTTCTCTTTCTCAACGTTCAACTTCCGCATTACAGTCTTTAATAACTGCATCTAAATCTTTTCAGCTTTTAATAACAGCTCCTGAGGCTCTTTCGTGGCCGCCGCCGCCTCATTTAACTGCAACATTTCTAACACAAGGACCACTTGATCTAAATTCAATTCTAACATTGCCATTTTCTTCTTCATTGAATTGAACCCAAAATGGATAACCAATAATATTCCCAATTGTATTTACTCTAACAACTTGATTTGGTTTTTTGCCTAAAGCTTTTGTTGTTTTTAAATCAATAGTTGTATAAGCAACTTTACCAACTGTTTTTAAATTATTTAACAATGTTTGATTAATTTTTAAATCGTCTAAAGGTGTAGCTGCTAGACCTAGGAATATTTTGTCAGCCTCTAAACCTTGTTCATATAAATATGAAGCTAAAAGGTGAGTGCGGGCATTTGTATCACCATATCTAAAGCTTCCTGAATCAGTAACAATACCTGTGAAAATGCAATTAGCAGCCCTTTTTGTTACTTTTCATTTGTTTGTATAAGCTAAGTCAGCTACCATTTCAGTTGCTGCAATTCTATCTGATTCAACTCATCTGGTACATTTATCTAAGTCATCACCATTTGGATGATGATCAATTCTAAGTGTTTCAGCAAATAAGTTTTTATCTAAAATTTCTCGACATTGAATTCTTTCTTTACCATTTGCATCCGGAATAATGCCTAAAGATTTTTTAAGTACATTATCTGAAGGACATTTATCATATTTAAGATTTAAAAATGGAAACATACCTTCATTATCGCCAACTGTGTAAACTTTCTTTTCTGGAAAATTAGCTCTAATTAATTCTGCTAAACCAGCTTGTGATCCTAAGCAGTCACCATCTGGTCTAATGTGATGAAAAATAACTATTGAGTCATATTTTTTAATTAATTTTGTTGCTTCTTTTAAGCTACCAATTTTCATGTGTCCCATTATTTTGCCTCTCATTCTTTAATGCAGTCATTTAATTCTTTTAATACTACTGGTACTTCATCTCAAGAATTAATTGAGCAACCTGCAGCATTATCATGACCGCCACCATTGTGCAATCTAGCAACATTATTAACTAATGGACCATTTGAACGAAGTCTACATCTAACTGTGCCATCAGCAAGCTGAATAAAGAATGCTCAACAAGAATTGTCATCAATGTTTGCTAATTCATTAACAAATATTGCGGCTTCTAAATCATTCATGCCATATTTATCCATAATTTCTTTTGTTACTTCAAAATATAGAACTCTGCCCGATTGTTTGAATCCTGAAAGAATTTCGCCTGTATATTTAATATCTCTTGAACTTCTTTTGTTTAATTCTCTTAAGATTCATTGAGAATCTAAACCACCTTTTTCAATTAAGTGAGCTACTAATTTATATGTTCTAGCAGAAGTATCTGGATATAAGAATCTACCTGAGTCTGTGTTTATTCCTAAATAAATATGTTCGGCAGCTTTTTTAGTGATTTCTCATTTTGCACTTTTAGCTAACATAGCCACCATTTCAGCCGCAGCTACATAGTGTTCATCGATCCATAAATAATCATATTCGATATCTGAACCATTTGGATGGTGATCAATTCTTAAAGCAGCTGTACATTTTTTTTCTACTAATAAATGTCCATCTTGAATCCGATCACTACTTGAAGCATCAACTACAATACCTAATGAATTTTTAAAATCAACCGTTTCAATTGGGTCATAGTGGTAACTCATAAAATCAAAAGTATGAACATTATCTCCAACTGTAAAAACTTTTTTCTTTGGATAATTCTTTTTTATTAATTCTGCTAAACCAGCTTGTGATCCTAAGCAGTCACCATCTGGTCTAATGTGATGAAAAATAATAATATTCTTATATTTTTCGATTGCTTGTAATGCAACTTTATAATTTCCTATTTTCATATTTTTCTCCTTAAGTTTGATATTTAATTATATTGAATTATTTAAATTTTTAAATTTAATTAATAAAATATTTTTGACCGTGTTTTGACTAATAACTCTGTTTTATAGCTAAATTAGTAAAATAAGCAGCATTTTAATTATTTAAAATTTATGAAAAAAATATGAAAAAAGGAATAAAAAAAATGGGGCGGATGAAGGGATTCGAACCCTCGAATGACGGGACCACAACCCGCTGTGTTAGACCACTTCACCACATCCGCCATTAAAATTGCTTTATTATTATAATATATTTTTTTAAAAAATAAACTAAAATATAAGAACTATGAATAAGAAAAGTAATAATGTACATAAATATGATGCTGTTGTTATAGGTGGAGGGCACGCTGGTTTAGAAGCTGCTTTTGCCTTAGCACACAAAAATCATAAAACTGTTTTGATATCTTTAAATAAGGAACGTTTAGGTATGATGCCATGTAATCCTTCAATTGGTGGACCAGCCAAAGGAATCATAACTAGAGAAATAGATGCCTTAGGCGGAATGCAAGGACTTTGGGCTGATTTAAGCATGATCCAATTGAAAATGCTTAATCAATCAAAAGGTCCTGCAGTTAGAGCACTTAGAGCTCAAATTGACAAAGAAAAATACATGAAGATAGCTTTAGAATACGTGTTAAAAGAACCTAACTTAGATCTTATTGAAGATATAGCTGAAGAAATATTAGTAAATAGTAAAAAAGAATTTAAAGGAATTAAACTAGCTAATGGCGATATAGTTGAAGCTGAGGTCTGTGTTGTAACTACTGGAACATATATGGACAGTAGAATTCTTAGAGGAGATGTTATTAAAATCAGTGGACCTGATAATGAAAAAACTACACCTAAATTAAGTGCTTCATTAAAAGAACATGGTTTTATAATTCAAAGACTTAAAACTGGAACACCTCCAAGAATCTTTTCAGATTCAATTGACTACTCGCAAGTAGAACAAGAAGTATTAAGTGATACTAACTTATCATTTTCATCTAGATCAAATGTAAAATTACCTCGTCAAATTGCTTGTTACTTAACTTATACAACTCCTGAAACTCACAAAATAATAGAAGAAAATTTAACTAAAAGTGCTATGTATGGAGGAATAATCAAAGGTATTGGCCCTCGTTATTGTCCTTCAGTTGAAGACAAAATTGTTAAGTTTCCTTCAAAAGAAAGACATCAAATTTTCTTCGAACCTGAAACTGCTGATGGTTCAATTACTTATGTTAATGGGCTCTCTACTTCAATGCCAGTTGAAGTCCAAGAACTAATGATTAGAAGTATTCCTGGTCTTCAAAAATGTAAGATTCAAAAGTATGCTTATGCTATTGAATATGATGCAATTGATCCTTTGCAATTAAAACCTACTTTAGAAACTAAAATCATTAAAAATTTTTATACTGCAGGTCAAATTAATGGAACTAGTGGTTACGAAGAGGCTGCTGCTCAAGGTTTAATTGCAGGTATTAATGCAGCCTTAAACTTAGAAAAAAAAGATGGTATAGTTATTTTAAGAAACCATGCTTATATAGGAGTTTTAATTGATGATTTGGTTACTAAAGGAACTAAAGAACCTTATAGAATGCTAACTTCAAGAGCTGAATATAGATTACTTTTAAGAAATGATAATGCTGATATTCGTTTAGCCGAATATGGCTATAAAATAGGCTTAATTGACAAACATCAATATCAAAAGGTTATTAAAAAATATGAATTAATTGACCAAGAAATTCAAAGACTTCATACTACTTATCTTTCTTCAAAAGATCCTGTGGCTATAAAATATGGAATCACAACAGGTATCTCATTATTACAAACTTTAGCTCGTCCTGCAGTTGATCCTTATGATATCATTCCTGATTTTCCATACCTAGAAGAATTAACAACAATGGTTAGACTTCATGGTTATATTGAAAAACAAAAAAGTGATGCTAATAAAGCTGTAAGACTAGAAAATTACAAAATACCAGAAGACTTGGACTACAACAAAATTAATAATATTGCTACAGAAGCTAAACAAAAATTAATCAAAGTTAAACCAACAACAATCGGCCAAGCAAGTAGAATCAGTGGCATTAATCCAGCTGATATTCAAATGCTTATGTTTTATTTAGAAACTACAAGAAAAAAGAATAATGCATAAGATTAAAATAATTGCAGTTGGTTCACTTAGTCCAGAATTTAAAGTTTTATTTAATCAATATGCTAAAAACATAAGTCATTTTGCTGAATTAAATGTTGTTGAAGTAAAAGAATTGAGTGAGGAAAAAAATATCGAAGTCAAGAAAGACAAGGAAACAAAATTAATTAATTCTTATGTTGATAATAATAGTTTTGTTATTTATGCTTCATTAAAAGGAAAAAGTTTAGATTCAGTTGAATTAAGCGAAATAGTTAATAACAATGTTAATTTAACCTTTATAATTGGCGGCAGTGATGGAGTTAATGAAGAATTAATTAAATGCAATATGAAAATTAATTCTTCAAAATTAACTTGGCCACATCAATTGTTTAGAGTTATGTTAGCTGAACAAATCTATCGTTCATTTATGATCATTAATAATAAAAAGTATCATAAATAATTACAAAAAACATTATTTAATAAAGAAAGGACATATGTCTGAAAAAGGAAAAAAATTTAAAAAGAAACGTACAGGAATGATAATTGCTAGTTTAATATTTCTTTTTATAAGTATTGGATTAGCTGGAGCATTATTTCCAATAATTCATATAGTTGTTACAACTAAAAATTCAATTGGTTCAATAATAGCCATTATCATATCAGCTGTTTTTTTCATAATAACAATTTGATTTATTAATTTTAGTATTGGAACTAATTATAAAGTTGTTAACTATAAAGATAAAACTATTGAAGTATTGGTTGGAGTTTCATATATTGAACTTTTAGTTGATGGTAAAACTGTAGATAAATTAAATGTTTTTAAGAAAAGAAATGCTTCATTAAGTCATAATTTAGATGGCGACCTAATTCATGTTAGAATTGATGGCTGAGTTTTTTATCAAGTAAATATAAAAATAAATAATGAATTGTTATAAACATCAATATTATTTGTGTTAAAGGCACAAATTTTATTTTGCTTTTTTTACTAAAAAGCTTATAAAACAGCAAAAAAAGTAGCCTTCTGAGCTACTTGATTATTAATAAAAGTTAAATTATTTTGTACGTTTAATTTCCTTAAGACGAGCACTTTTACCACTTCTGTTTTTCATGTAGTAAAGTTTTGCACGACGTACTTTATTTGAACGAACAACTTCAATTGAAGCGATCATAGGTGAGTGTAATGGAAATGTTCTATTAACACCTATACCAAAAGAAATTTTTCTAACTGTAAATGTTTCACGTGTACCTGATTCTTTTTTGCTAATAACTAAACCTTCAAAAATTTGGATACGTTCTTTTTCACCTTCACGAATACGAACGTGAACTTTAACATTATCTCCAACTCTGAATTCTGGGAAGTCTGTACGTAATTGGTTTGATTCTACTAATTCGATTAATTTATTTCTCATTTTTAATCCTTTCGATTATATCTGGACGATTTTTCTTTGTCTTTTTTAATTGAGCTTCTTTTTTCCATTTTTCAATTTCTGCATGATTTCCATTAACTAAAACTTCAGGCACTATTAAAGCTTCTTTTTTAGCTTTGCTCTTGTACACTCTTGGTCTAGTGTATTGAGGATAATCTAACAAACCTTCATTTTGAAATGAATCATTAGCATAACTTTCTTCTCTTATTACGCCAGGAACTAATCTTATGATACTGTCTGCCATTACCATTGAAGGTAATTCGCCTCCGGTTAAAACATAATCACCAATTGAAATTTCTTCATCAACAAGGTCTACTATTCTTTCATCAAAGCCTTCATAGCGACCAGAAATAAAAGTTATTTCTTTTTTCTTAGCTAATTCATTAGCAATTTGTTGATCAAATTTCTTTCCTTGAGGAGTTACAAGAATTTTGTAACCTCCTCTTTCTTTAAGAGAATCAAGTGCTAAATCAATAGGTTCTATTTGCAGGAGCATTCCATGACCTCCACCAAAAATTTCATCATCAACTTTGTGATGTTTATCTTTGCTGAAAAGTCTAAAATCAACTACATTAATTTCAATCAATTTCTTTTCAATAGCTTTTGAAATAATTGATTCTTCTACAAATGGTTTGTAGTAATTTGAAAAAAGAGTTAAAAAATTTATTTTCATAATCTAGATATAACTATTTTTTTGTAGTTTTACGAACAGTTTTAGCTGAAGGTTTAGCAGCAGCTTTTGTAGTTTTAGTTGTTGCTGCTTTTGTTGCAACTTTTTTGTCAGCAACTACTTTTTTAGGAGCAGTTGATTTTGAAGCAGGTTTTGTAGCTGGTTTAGCTGCAAGTTTTTTAACTTCTTGTGCTTTTGTAGCTTCTTTGTGTGCGTTGTTCATGAATTTAACATTTAATTTGTGTTGTCTAAATAAATTGAAAACGGTTTGAGTTAATTGAGCACCATTGTCAATTCATCTTTGTGTTGCTTCTTCATTTAAAACAAATTCCTTTGAGTGTGGATTATATTGGCCTAATGCTTCAATATATTTCCCATCTCTAGGAGCGTGTGAGTCAGCAGCCACAATTTTGTAGCAAGCATTAAATTTGCTTCCTAATCTCTTTAATCTAATTTTAACCATTGTGGTTTTTCTCCTTATTTTTCTTTTTATCAATAACTTAACTTGCTGTCAAGTGCAAATACTTGACACCCAAGCTTATTGCTTTATTATTTTAATATAAAATATAAAAATACAAAACAAAAAAATAAAATGTTTTAGATACTATCAAAACTAATAAATGTAAGCATAATTAAAAAATAAATTTTTTATTATAACCACGCTTAATATAAGTTTTAAAAAAATTTAATATAATTACAATTATGAAAAATAATGACAAAAGAATAATAAGCCGTCCATTAATGGATGATACAGTTGAATTAACTAAAAGTGAAAAAAGAATTCGAGATTTTATTAATAATTTTGAAGGTAATTATTTTAACTTTTCACAAAATAAGTTAGCTAAAAATACTGAATCAAGTGATGCGGGAGTTTCTAGATTTATTCGTAAATATGGTTTTAAAGACTATCGCACTTTTATTGCTTCAATAAATAACAAATTAACAGAATTTGAAAAAACTTATCCTACTATTTGAAATGACAATGAAGTAGATAATGACTATAAATTTATTGCAGCCAGTCATAGATATGCAATAGATAATGTTTATGATGAAGATCTATTAAAAGATATTAACAAAGCAGCTGAAATAATTAATAAATCAAAAAACATTTATATTCACGGTTGTGGTTCAAGTCAAAGAATATCAATGAATTTAGTTTCTAATTTATTAAAAATTGGTAAACCTGTTATAGCTCATAGTGACTTTCATATTTTCTTTCCATCCTTAGCTCATGTTACTGAAAATGATGTTGTAATTGTTTACTCAAATAATTTACAAACAATGGAAGCTCACTTTGTTATCGAGCAAGCTAAAAAACAAAAAGCGCCAATTATTGTTTTAACTTCTAGCCAAGAAGAAGATAAATTAATTGCAGTTAAATTAAGATATCACAAAATTCAAAGTTCAACTATGTTAGTTCCTTCAAGTTCAAAAATAGCTCAAATGTTAATTACCGATTTATTATTTGAAGCTGTTTTAGAGCATAATAAGGAAAATACAAGTAAACTTAAAAAAGCAAGAATACTTATTAAAAATTGATCATTAATAGATAAAGCTCATAGCGAATAAAGGAGATATTATGCCTAAAAGTATAAACACATTAAGAAGAGAATCACAAATTAGAACATTATTAGCAGAAATAATAACAAATGATTTAACAAATACAAATATTATTAATCCTACATTGGTTGATTGCGAATTATCAGGTGATTTATCACATGTAAAAATCTATATGTCATTTAGCGAAAATGAAAAGAGAGGATTAGAAGCTTTACAAAATGCTTCTGGATACATTAGAACAATTTTGGCTAAAAGTTTAAATTGAAGAAAAGTTCCAGAATTACATTTTTACATTGATGAAGTAAGCAAAAAATGTTTATCAATTGATAGAATTCTTTATGACATTAAAGATGAGAAAAATTTTGAAGCTAATAACAAAATTTCTTACAATGAACACTTAACAAATTTTGGTAACAGTAAAATTTTTGAATTAGATAAATATGAACCTCAAATAGTTTTGCATACCTTAAATAAAAAGAATGAATTAATGAAAATAAGTTATTTAAAAAATGAACAAAATGAAAAATCATCTAAAGCTTGGTATGTTTTAACAACAAACAATTCATCTTATGGTCTTTTTGGCTTAAAAAACTATAAAACAGATGTTTTGGAAAAAAATTGTTTTAAAAAAATAAATTTAAAAAATAGATCTTTTATTAATTTTAATAATGACTTTGTACACTCTGCTTATACTTCAGACGATGAATTAAGATTATTAGAAATATCAGTTAATACTAAAAAAACTGAAGTAAAAAATTTTAAAGATTTAAATAAAATTAACAATGAAAATTCATCTACATCAATGAATAGATATTATAAGGAAGATTGTATTGTTTTTTACAATAAAAAAACTAGAATTACATTATTTAATGATAATAAAGAATATATCGTAAAAAGAAAATGTTTAGTTTTTGATTGCATCAGTGAAACGCTTTATAGAGCAAATAAAGGCGATGAACTAAACTTTCATAAAATGTTAATAATTGAAAAAAGATAAAAAAGAGCGATTTCTCACCCTCAATTAAATTGGAAACCTTAAATGGCTTTTTTATTTTCTTTTTAAAGCTAAATCTTTTAAAAAATCTCTTAGCATAAAAATACAAAAGTATGGAAAGGTATAAATATTATTTTTAAATCCGATGTTGCTGTTAGCAAATTTAATTCCATATTTAACATTTTTATTTTTGAATAATTCTAAAAAGTTTTTAAGACTTGTTGCTTTTTTATTATTAGATTTAATTTCTAAAGGGACTATATAATCAATATCTCTAACTATAAAATTAATTTCAATAGTGCTTTTTGGATTCTTTCAATAGAAAAGATCATATTCTTGTTTTTTTAATGATTCAGCTACTATGTTTTCATAAATAGCGCCTTTATAAGTATTTAAATTTTTTTTAATTCTTAAATCATCTTGTGTTTCTTTATCTAAAGAAGCTATTAATAAAGAAGTGTCATGGTGATAAATTTTAAATTGGTTTGAATCATAATTAGCTAAAAATGGAAATTCTACATTATTTAATAAGTATGAAATATTAATTAAGCCACTATCTTTTAATCATTCCATTGTTGAAAAATAATCTCTAAACCTTGCATTATGTTCAACTTTTGAAAACATAAATCTTTTGTTTTCTTTACCTAAAAATGGAGAAATACTTTCATAAACTTTTAAAATTTTGTATCTATCCAAACTTTCTGCATATTTACCAATGTCTTGCTTATAAACTTCAAGTAATTCTTTTTGTTGATTTAAAATATTTGAAAAATTTTTTTGCTTTATGAAATTTTTAACAATCTCTGGCATTCCTCCAACAATAATATAGTCATTAAATTTTTCAAATAACACTTTTAATTCATTTTCGCTGAAAGGTTTAAGTTCTTTCATATTGTTAAAAATTTGCTTTATTTGTTTTTCATTATATCCATTTGCTCATAAGAATTCTTCAAAATCTAAAGATTGCATTGTATATTCATTTTTGTATCCAGCACTATTGCTGGTAATGTATTGATATTGAATACCTAATAAAGATCCTGAACATATAACATCAAATTTTTTATCTAACGCGAAAAATTTTAGACATGTTGAAGCATCCGGATATGCTTGTATTTCATCTAAAATTATTATTGTTTTATTCTTTACAAATTGAAAATTTGGATTTACAAAACTAAGCTCTTTAACAATTGTCTCAACATCAAAGCCTTTTTGAAAACAACCTTGATATCTTAAATCACTTATAAAGTTTATATAAACAACATTTTCATAATTATTTTTTGCAAAATCTAAAATAGATGTCGTTTTGCCAATTTGACGAGCTCCTTTTATAATAAGAGGTTTTCGTTCTTTATTTAATTTTCAATCTTGTAAAAATTTATCAATTTTTCTTTTTAATAAAGCCATATTTGCCTCATGTTTATTATATATAAAAAATGTGTTTTTTCACACAATTCCATATTTTTTTTGGTAAAATTTCACACAATTCCATATTTTATAATAAAAAAATACCAGTTTTATGGTATTAATTTATTGCATTTTTGCTTCAATATCTAGTAATTTTTTAATTGCTTTATTCACACTATCATAAGCTCCAGAACGGGTGGTTGCTGTAATGTTGGCAACTTCAGAATAAGAAAGATCTTCAATGAAATATAATTGAAAAGCTTGTTTTTGTGTTTGAGTTAAAAATGAATTAAATTTATCAAATAGTTCTAATAAGTGCTCTCTATTTTCTATATCTTTAATATCTTTATTCATTTACAATTAAGTCCTTTGTCATTTCATAAATGAAGTTATCTAAATCAAATTCTTGAAGATCATCTAGCCCTTCGCCAAGTCCAATATATTTAACATTCAAATCAAATTCGTCTTTAATTAAAAGTACAATTCCACCTTTAGAAGTTCCATCCATTTTAGTTAATATAATTCCAGTTGGAGCCGCAACTTCTTTAAAACTTCTAGCTTGGCTAACTCCATTTTGACCAGTTGTTGCATCTAAAACTAATAAGCATTCATGTGGCGCTTTATCTTGAAATCTAGCAATAATGCCATACATTTTTTCAAGTTCTTTCATCAAGTTGACTTTATTTTGTAATCTACCAGCTGTATCTATTAATAATAAGTCATATTTTTCATTTTGTGCTTTTTCAAGAGCTTCATAAACTACTGATGAAGGATCAACTCCTTCTTTAACAGGTTTAACAATAGGAGCGCCTACTCTTTCTGCTCACACACCTAATTGATCTACTGCACCAGCTCTAAAAGTATCGCCTGCAGCAATTAAAACTTTTTTTCCTTCTTTTATATATTTATAGGCTATTTTAGCAATTGAAGTTGTTTTACCACTACCATTAACTCCAACAAAAATAAAGATATTTAATCTCCCGTCTTGATAATTTAAAGTTGTATCAACAATACTTTTGTTTGTATAAACAACAAACATTTGATCAGCAACTAATTCACCTATATCTTTAGTATCTTTTAAACCTCTAGCTCTAACTTCGTTTTTAATGTGTGTGATAATTGCATAAACTAATTTAGCATTAATATCACTCATGATTAAAATCTCTTCGAGTTCATCGAAGAATTCTTCATTTATTTCTTTATGTTTATTTTGAAGGTTGAAAATTTGTTTACCTAGAGAAGAAGATTTAGAGAGACCTGTAGCATATTTATCAAGCTTCTTTGAAGTTATTAATTCTAATTGTGCTTTTTCTTCTAATTCTTTTTGCTTAGCAACAACTTTTTCTGCTTTCTTTTCTTCTTTTTTCTTTTTTCAACCAAAAAGTTTTTCTTTTAAATATCCAAAAAATCCCATAGTGTTTTAATTTTATATTATTTTGGTTATTTTAAAAACAAAGTGTTATATACTGTATATAAAAATAAGATGTTGCCATCTTAAATTTATATTAATTTTGATCTTGTTATTATGTAATCTTTTAAAAATGAAATTGCATCATTATAACTCAGGAATGAAAACTCATTGCCTTCAAATTTCAATACAAACACATTGAAAATGTCATTGTTAATTATTTCTACTACACCTTTATTATCATATGCAACTAAATCTTCTTTATACCCTAATTGATTACTAAGTAAATATCTCAAATTCCCATCAAGTATTTCCTTTGTTGGAAATTCTTCATTATTTTCTAAGGTATATGAAGATAAACGATAAATATATGCAGGGTGTCAATTTTCGTCAAAATAAAGAGCTATGTTATTTTCATAATCATCTTTAGAATAAAAAGTTGGAAACTCAAGTAGTATGTTAGAAGGTATTGAATATCTTTTTGATAAGATGTCAACATTATTATTTGAATAGAAAGAAAATAATTCAATGTTTTTATCATTAATAAGTAAGGGCAAATTATTTTCAAAAAAGCTATTATTTAATTTGTTTTTATTCTTATGTTCTTCAATAATTTCTTTTAAAAGTTTTTGTTTGTCTTCGGGTTCAGAAGCACTTGGAAATGATTTAGAAGAAATTATTTTGTTTAAAAATTCTATACATGTTTTTCTTTGAAAAATCAAATTTGAAATATTAGAATTTTTATTTTTTTCATATAAAGAACAATTTCGGTTGTTGGCAGTTAAAATTTGACTTTTATCATCATTTATTAATCCAATATTTCCTGTAATTGCATAAAATTTATCATAAATATATTTGTTATTTAATTTAATATCATTTATTTTTGAAAAACTAAAATCAAATTCTTCTCATATTTTATTCAAATTAATTGGTTTATAATCATCTCAATCTATTTTGTCTAAATCAATTTTTTTTGAATAGTCATTACCATTTATTATCAAATTTTTATTCATATAACTATCAAAAGTTATGACATTATAATTTTTTATTTGATTTAAACATTCATCATTTTTAAGAATATATTCTATATATTTTTTATAAACTGTAACAATTTGCTCTTTTTCTTCAATTGATAACGAATCAAAATTATTATTTGAAATTTGATAAGTAGAAAAGAAATTGTTAATGTCAATGTTGTTTAATTCTTCAAATGTGCTCTTTTGGTCATAATAAGCCTTAATTCTTGGCCCAAATGAATTAACTGGTCTTGAATAATAGTGCTCTTTTTCATATCCAACAAATGTTTTGACTTTTAATTTTTTATGAATCGTATAATTTGCATCAATTAATCAAATAGTGCCTACATACGGTTTATTCATCGCTATATCATTGAAAATTAATTCGACATTTTCTGTATCAAATAAATTTAATATGTTGGCTTTTACAATCCAAGTTCAATCTTCAAATCTTCCTTTTTCGTGGTCATAATAATTTTCAACATTTTTTGAAGTTGCATCTCAAAAACCGTTATGATTTTTCATTCCATTTTTATAAAATTTGTAAAATTCATTTAAATTGAATGTTCTGCGCAATTTATTGCCATCATAAACATTTCATTTATTATTTTTTACATTTACTTTAAAACCTAAATCCTTTTCATTGTAGTCATAAGACTTTTCATCATTGAGATCTAAACCAAATTTTATTTCTACTTTAAACTTCATTAACTTTTCAAGAATTTTTTTACTAATAGAATTCATTGTTTCATAATTAAGTAAATTATTATAAATTACATTATTGTTGTTATCAAAAATAGGATAAAACACATTGTTATCTTTATTATATTTATCTTTTAGCAATTCTAATGAATCTTGCCAATTCATAGCATTATATTTTTTCCCATTATTAGAATAATAAACATATTTATTAAAACCGTTTTTAAGTTGAGTTTTTATATACTGTTTTACTTTTTCTTCTGAATCAAATTCTTTATTGGCAAAAAGATATTTTTTAATATATGAGCTTTCTTCTCTAAACGATTCTATTACTTCATCAACTGAATAAACCGTTTTTTGGAATTTATTTTGATAAACTTTTTTAAGTTTATTTGGGTCGAAATTATAAAAAATAGTAGAATCTAGAATTTTATTTGAACTATTGTTTTTCTTTTTGCCTATTACTAATTTGTTTTCAACTTTTATTTTATTACTATTTATTAAATTAGACATAAATGATTCAATATCAGTATATTGGTATTTTTGTCCTTCTAAATTAATTTCATATTTGCTTGCGTTTTTTAAATTATTGTTTTGCATATAATAATTCATAGAGGCTGAATTATTGCATGATGGAATCAAAACAACTGTTGGAATTAAAGATAAAAATAATTTTTTAATTTTCATTTTCGCCTCTTTTATTATTCTTTTTGTATCAAGATACTGCTTCATTCAATGTACAGAACTCTTTATTCATAAATATAATTTTGGTTCTTTTGGTTAAATTATATAATTCCATTTTTGGGTTAATAATAACATTGATAAAATAATTTCTCGCTTTGTCTAAATTTGTAAAGAAGTATTTATTGTTTTTATAATCAAAATAATAAAGTGTTTTTGTTTGCAACAATTCCATATCTTGATAATTTGAATTATTAAAAAATGATATATTTGAAACTTCTTTTGTTCTTATATTCAATGAATTTAAAAATGGAATAAATTTGTTTCTATTATTTTTTAACAAATCGCTTTGAATATTAAAGTTTCCTATTTCTGAATATAAAAAGCAATTTTTTAATGGATCTTCATTATAAATATTTGCTAGTATTTTTTTGTCTTCAAAAGCTTTTTTTGTTGGATCAAATTGATCAATAGGAAATAGCTTGTCGGTGTAACCATCTTCTGTTTCTATTGGTTTTTTTGAATAATAAATAGGATTCAAAATGTCGATCGCTTGTTTAATTGAATTTAAAATGTCTTTTATATCGTCTGTTTCTTTTGCTAAGCTAAATGGTGTGCCGTTTTTATAATTAACTAATGACGAAAATCAAGAATCTTCATGTTCACCAAAATTTGCTTCAATTATATTAAACCCATCATTTTTAGCTCAAGAATTATATTTTCTTAAAAATTTTGGGTTATTATAATCATTAAATGAAATTTTTTGTTCTAATTGTTTGTCTCATGAGTAAATAATTTTTATGTTCTTTTCAGCTTCATCTTTTTCTAATAAGTTGTTTATAATTTTGTTGTTTCCTTTTAAATAGTAATTTACAACATCTCTTTTTAATAAATCTATATCAACAAAGTTATATTTTTTACCATTGAAGTAATAAAAACTTTCAGTATAACCGTCCATTATTTTTACAGGTGGTAATATTTTAGCATGTTGTATTCCAGACTCAACATTTATTCAATGAAATAAACCAACATAACGCAATCCACCATTCCATATATAATCGACATTACCTTTATCTCCTGCATTGACAACGTATTTATAGTTAAATTGGCTAAATTCACCAAATAATGCTTTGTAAAATTTAACATAAAAGTTAGACAATAAACCACCAAAAGCAGAAACTGGACCTCCGACAACATTTAAAGCTATTTCTAATGCTGCTGCTTTTGCATCTATTATTGAATGAATATATTTTGTAGTTTCTACTAAAAGATTAACTTTGCTTGACCAACTACCTTTTTTAACTATATTATCTATTTCTATGAAAGCATATAATAAATATTTAGCAAATGATTTTTTTTCTACTGGCAACAAATCTTTTTTCATTTGTTCATATTTTTGATGAATAGTGATAAATTTTCTACATATAAAAGTAAATAAATTTTCATTTTGAAGCTTTTCATTATTATAATAGTTTAATAATCAAAAACTTTTATCTTTGTTTAATAAATCTTCATTATTTAGAAGAATATCTTTTACTAATTCATTCACATTATCAAAGTTGTTTTTTACTGTCGACATTAACTTTTCTAAAAATCATTCTTGTAAGCTGTTGTTAGTATCATATGATTGTTTAGTAAATTTGTCATAATTTTTTGAAAAAACTGGTTTTTTAAATCGCTTTAAACTATAAATTTCAAAATAATGTTCATAATTATCATATATTGCATATTCAGGAAAAATGAGAGATTCTGGATCATAATCTTCACTTGAAAATCTATATACTGCATTAAATATGCCACTTGCAATTACATCCAAAAAATTGCATGCTATTTTATATTTCAATAATTTAGCAATCAATATATTTGAATCTGAATTTAAGTCAAATTCCCCGTTTGTTATATCTCAAATTTTTGATATATTTACTTGATTATCTGAGTTTTCTGATTCACTAATTCCTTCATGAATGAAATATTTTGGTAATAATGATGCAAAAATTTTGCTAATGTCATTTATGATCATTTTAAAATATGTTTTATATTCATCAACCACTTTTTGATTTGCTTTATATTCTATAATTTTGTTAAACCCGCAACATATAATATATAAAACTAGAAAGAAAATTAGATTCTTTCCTTTTGCTAAAAGATCATATTTGTTATATAAATAATCGTATAGGTTAGTGCCTTCAAAACAAAAGTTTTTGGTACATTTTTTGAAATTGTTTAAATGATCTTTTTCAACATTTATTGTTATAGGTGTAAATGGTGTAATTTCAACATTTTTCTTTATGTTTTCATTAAAATACACAATTTTGAAAAGTGATGTGACAATACTGTTCATCTTTTGTATTTTTAATCAATTAAATATTTCTTCTTTAGAATGATTATTTGTTGAACTTCATTTAGTTGTATCAGTTATTTCTTCAATATTTTCAAAAGGAGTCTCTAAAATTCTTGGCCCATACAAAGTAAATTCATCATCTTTGGAATTGTCTACATAATATTTGTTATATCCTTTATTAGAATTAATTCTAGTGATTTTACTCTTAATAAATTCAGAGTTTGATATTCTATCAAATATATAATCTTCAACCGCTTTTAAGTGAGGATTATTTTCTTCATCATAATAGATTACATATTTCTTTGCAACATTTTTAATGTAATCTTCAATTCTGCTTTTTTTGTCTGCATCTTTAAGGTCTTCTTTTTTAAAACTTGTAATTTGTCCGCTTGGACTTTTGAAAAATTCTGTAATAGTTGCGTTAGGTGAATTAAAATTCAATTTGCTTTTTATTTCATTTAATACTTCTATCAAAAAATCTTTATTATTTACTGATTTATTTAAAATGAAATTGTAAAGTTCCTCTTTACTGTTTGCATAAATATTGCCTAATTTGTAAACTTGATTTATATTAAAATATGAATCTCTTGCTTTTTCTAATGAAGTAAAAACTTTCCCGTTTCGTCCTAAAAATATCGGCATTTTTTCGTTACTTGTTGACTTGCTAAAATCAAAATATGAAAGCTCTTAATTGGACATAAATTGTGGATTATTTTTATTTATATCATCAAATTTTTTTGAGTTTTTTAAAAATTTCAAATCAACAGATTTTATATTATTTTTAACAAAATTTTCTAATTGTATGTGGTTGTCAAAAACTTTGTTTACATTTTGACTAATATATCTTGTTTCCTTAATATTTGTGTCTCTTGTTATAGAGTGTTTCTCAACATATTCATTATCACTTAAAACTTTTTCTTGCTCTTGTTCTTTAGAACCACAAGATGATGCGACAACAAATGATGCCCCCATCATTATTGTTGAAATAGATAGTAAATTAATTTTTAATTTCTATTCATGTTTTCTCCTTTTAAAAGTAAATATTATTGGCAAAATGCTGAGTGTTAAAATAACAAATGTTATTAAAAATAAGTTTATCAGGTCATATGTCAAATTATTTAGTTTTATTATTTTTGTATTGTCATAAAAGTTTTTGGGATTTACGTTTTCTTCAAATTTAAACGCTTGATAATTTCCTAAATTAGATACAAGTTCATTCATTTTATCAATTGTTTGTTTAATAATGAACAAATGATTTGAAGAACTATAAACAAGATTTTTTCATTTTTCTTTTGAATCTATTTCAGGAAAATATAAATTGAATTTAGTAATTGTTGTTGTTATTGAATTTTCAAGTTCACTTTGTGCATATTTATTAAAATCAATAATAACTTTATCACCAACAAAATTCACCATTTTTTCGCTAATAAAATTTAAATCTGAATCAGTTATTTTACTATTTGAATCTAAGTATTTTATTAAATTATTATCTTTAACAATATAAAATTCAGCCATGTTAATTGTGGTGTTAAATTTTGTTTGATTTGATCTGTATAAATGATTTAAAAAATCAAAGAAATTAATTTTGAACATACTGTTTAAATTTGACTTTAATTTTGCAGCATTTCATAAATTATCATTTCAGTCTCTTTGCAAGTATTGAAAATTAAAATCATCTTTGCCAAGTAAATGCATTACTAAATTGTATTCATTTCCAGTAAAGTTGTTTTTTATTCATATATTAGATTTTATTTTTTTAAGTAATTTATCTAAATTAACTCCACCTGATTCAAAATATTTTTTTAATTTGCTATATAAATAATTAGCCAATTCATTTGTACTCATTTTTATAAAAAACTCACCATCGTTATGATAAATTCTAAATTTATCTTTGTCAAATTTTTTATTATTTTTTAGAATAGACAAATTGAATGTTGAGAATATATAATTTTTTGTATCTGAATTATTTTTATAAGTCAAGAAATTCTCATCAAAGTACTTATTTATAGTATTTTTGGGTAAAAAATATATAGATGAAATTAAGTTCAAATTATATTTACTTTTAATCTCATCATCTGATAAAAAGTAATACTTGTCACCAGAAACTATTATATCCTTGTTTATTTCTACCTTTTTATTTGCTCGCGCTTCTATTGTTTTTGATGATAAAGACACAACAGTAAAAAAAGGTAATATACAAGTTGCAATGACGAGAGACTTTTTATAAGATTTTGTTATAAATATAAAACTTGCAAAAACTAAAATAATAAAAATGATTATCAATAAATTACTTAGAAAATAGCCTATAAAATAAAGTAGAAAACTTTTTATATTCGTGTCTCTATATAAATGCAATGAACTTATTGCAAAAGACTTTAATAATGAAAATGCGGATAATAAAATAAATGAATATATTGACCAAGCTAAAACAATTTTAGGTTTATTAAAATATCTACAATTTAATGTGTTTTCATATATCGATTTTTGGTTTGATACAAATGTTAAATGCAGAATAAATAATGTTGACACACAAACTATAATAAAATCCAATAGAAAAATCATTCAAAAATAATTATAAAAAAGATAATTATCAAATAAGAACAAAGAACAAAATATAAAACTGATTATAAAAGAAAGTGATAAACCAATATATACTCATTTGTTTTTCTTATTAAACTTAAAAGTTAGAGATAGTATTTTTTTAGTCATAACCACCTCTCTTAAAATTATCAAAAATAATTTTTAAATTTTTTGCTGATTTTACTTTTTCACTTAGAACGATAGTTTTGTTATGCAAAATTGTTATTCTATCTATATCTTGCAATTCTTCAGATATTAAGTGAGATGATGCGAAAATTGAAGTTCCTTCTTTTTTTAACATATTCAAAAGTTTAAATAATTCATTTCTTGAATAAGGATCTAGATTAGCTTCTGGTTCATCTAAGAATAAATATTTTGGTTTTACTAATTTAGATATTAATAGCATTAATTTTCTTTTTTCTCCAGAAGATAATTTTTTAGGAAGATAGTCCTTTCTTTCATCTAAATGGAATTCTTTTAATGTTTCATTTATAAATAATTCATTTGTTTCTAATTCAAAAATTTCATTCACATAAAATTTAATTAAATCAATAACTTTTAAATGATTTGGAAATCTAATATCTGTTGGAACAAACAAATAATTATTAGTTTTATTTGATTTGTAAATATCTACATTATCTTTTAAAATTTTTCCAGAAAATTCTGAATCTAATCCACAAATTATTCCTAAAGTAACTGTTTTTCCAGCACCATTTTTGCCCAAAAAAACATGCAATTCATTTTCATGAATATTGAAATTTAAGTTGTTTAAAATCAATCGATTTTTTTTATCTTTTTTTGATAAATTAACTATTTTTATCATAAATGTAATTATATATAAAAATCTTTCAATAAGAAACCAAAACATTTTAGTTAGATCTCGGTGTTAATTCCAAAAGTGAGACCCCTAGGTTTTAGAGATCTCACTGCTACATTGTAATCTGAAACTTTACACATTTTAGATAAAATCTATAATGGGTGAAGTTTTTTTATAAAAAAAATCACCCAAAGAGTATTTGGCGACTGTCATGAAGAAATGCAAAAAAAATTATCATGAAATGAACGCACTGTTCTTGAATACTTACTTTGAGCAACTAATTATAGTATAACAAAAATAGCAAAAATATTAGGTTATTCAAGAACAACTATTAATAATGAAATAAAAAATAATTCTACTTTTTGGGGTTATTTTGCTTTAGATGCAATTGAAAAAACAATTACAAGAGAAAAGTGAAAAAATCATTTTAAATTTTTAAACAATTTAAATAAATATAAAGAATTTTCTTCTTTATTTAAAAAAGAATATGATGGCAAATTGTGAACTGTCAAATTAACAATTGAAAAAATAAAAAATCTTTATCCTAATATTGATGTTCCTTCTTTTAAAACAGTCTACAATTGAATAAAAAGTGGTTATTGAATTTTGAACTGAAAAGGTTTATTAAGAAAAGTCTACAAAAAAGGTGGAAAAAGAGAGAAACAAAGCGCTGCTAGAAGATTGGTTGGCGCACGTTATGTAAGACCTTTTTGATCTAGACCAGCAAACATAAACGAAAGAAAAGAATTCGGCCATTGGGAAATAGACCTTATTATAGGCAAGAGTAAAGGCACACATTGTCATTTACTTAGTTTTACCGAAAGAGTCTCTAGATATGGGTTTTTGGTAAAAATTCCTGGTGCTGTAAACTAATTGTGGGAATGAATTTAAATGAGAGAACAAGCATAAAGCTTGTTTTTTTCTTCTTTCTATCAAACTAAATATAGAAAGGAGGTGAAATTATGTCTTATACAGGAAATTTTGATACTTTTTCAACATTCTTTCAAAACTATGGATTAAATTATGACAAGTTAGAATCAGAAAAAAAACAAATAATAATTAATAAAAAATTATTACCAGAGAAAACGGCAAATTCTAAAATTTCTGAAACTAAAACTGAATCTTTGTTCAAAAAGATTAGTTCAAAATCTTTATTTGAAAAAATGTGAAAGAGTAAAAAGTATTTAAATTTTGCTGATAAGGCATTGATTTTTTTATCTACTCTATTCAAAATGAAAATACAATATCAAAAAGAATTTTTGATGAATTAGTATNCATGAATTCAAGGTATGTTTTATGTTATATTTTTTTGAATATCAGGAGCTAGAAGAAATTGTAGAATAACAGATAATGAATATGGTTTGCTAGAGTCATTATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATAAATTAAATGTTAAATCTATAACTCAGGATAATGGATTAGAATTTTCAACATTATTTTTCATTGGATATAAACTAAAAATTTTTATATATAAAGCTGATCCATATGCTTCTTTTCAAAGGGGTTCAAATGAAAATTTCAATGGTTTAGTTAGAAGATTTTTTAAGAAAAAAACTAATTTTGATGATATTACTGAAGAAGAAATTCTAAAAGTACAAAATGAAATAAACAATCGTCCAAGAGAAATTTTTGGATATTTATCTGCTTTGGAAATGTACGAAAAATTGAATGCAGAAAATCCTTGAAATTCAACAGGTATTGACAAATTATTATATGGAAAACAAAAGAGAAACTGAGAAAGTAACAAGAATAGAAATTTGTTTTTCAAAAAAATAGGAAAATTAAAATAATATTTTAGAAAGGGCGACAAGTCGCCAAATATTTTAGGGAGGGCTAATGCCCTCACTTTTATTTGTCCTATTGGACAAAAAATAATAACAATATTAATAATTAAAAAGGAATATCGACATGCGATATTCCAAATTTCTAGGTGTAAACTTACAGATTACAATCTAGGATAAGAAGGTATTGCCTTCTTATTTAATTATTATTTGAGATAAACAAAATATTTACTTTTCCATATTGTTTTTGTTTTTGTATTGTAAGCTTTTTTGGAATATCAATCATTAAGAAATTGTCAGTTTCTAAAATAATTAAACCATTTTTAGTTAAAAATTTATTATCAACTATTAATTCAAGAGATTTATTAATCAATTGATATTCCTTGTATGGAGCATCAATAAAAATAAAATCAAATTTACGTCCCGAATTCTTTTGTAAATAATTCAAAACATCATCTTTTACAATATCGATATTTTCAGCTTTTAGTTTTAAAGCATTATTTCTTATTGCTTTAATTGCTTCATTATTATTATCAACAGCTATTGCTTTTACCGCATAGTTGCTTAATGCTTCAAAACTAAAAGCACCACTACCTGCAAAAAGATCTAAAATGCAAGCACCTTCTAAGTTAAATCTTATTGATGAAAAAATAGCTTCACGAACACGATCCATAGTTGGTCTTGTTTCCAAAGTTTTAGGTTGTTCTATATTTAAATGACGATATTTTCCACTGATAATTCTTAACATTTTATTCTCCAAAATTAAAATTGTTACTTACATTATATTTAGTATTTAATACTTAATATATAATATTATTATGAAATATGAAATAAAACTAGTTGAATTACCTGAAAAAATTTTAAGGAAGAAATCAAAAGAAGTCAAAATTCCTCTTTCAAAAGAAGATGAAGAATTAGCTAAAAAAATGATTTATCACATAGACGAAAGCCAGAAAGAAGATTCTAAATTAGGTTTTAGACCTGGTGTTGGAGTTGCAGCTATTCAATATGGAATTCCAAAAAGAATGTTTTATATAAACATTAACAATGAAACAATAAATGGAGCTAAAATTGAAGACTTTAGAGATGTATTAATTAATCCAAAAGTTTTAGCAACCAGCGAATATGAAGTTTCATTACCTGGCGAAGGTTGTTTAAGTGTTGGGGACAACATTAAAAATCAAGAAGGCTATGTTTATCGTAAGAAAAGAATCGTTTTAGAAGCTTATTCATATTTTGAAAAGAAAGTGAAGCATTATGATTTAGTTGATTATGCAGCAATTGTTGCGCAACATGAATTTGATCACCTGGAAGGAAAATTATTCATCGATAGAATTAACAAAAAAGATCCTTTTGTAATCAAAGATGATTCAGAAATTTTAGAATAATTAATGAGGTATTTATGAGAACTTATGAAGATAATTGAAAGGCATTAGCAACTAATGGATGATGCGTCTTAATTTCCATATCTGTCTTTTTGATAGTCCTTTTGGTCGCAATTGCTATTGGGGCTAAAAGAGGATTTTATGCAGGTTTAATGTCTTTAGGATTTAACTTTTTAGGCTTTATCTTAGGTATAATTGTGGCTCCTCTTATTATTGAGCAATCAATTAGTACCAAACAGTCTAATTTAAATTTAATGAATAAACTTAAAACACTAAAACCGATGTTAGTTGGGGTCATAATTTTAGGTTTCACAGCAATTTTTGGTCTAATTGGTGAAATAGTATTTCTTTGTCTAAAAGCATTTTTCAAAAGACCAATTATTAAAAGAAAAAATAAGGGCGAAAGCGTAATATTCTACCGTTCAATGGGAGCTATCGCCTCAGGTTTAGCTTTATTACCAGGTGCAATTCTAATGACAAACTTAGTAGGTTTTGTTGATCAAAACTCTGCAATTAACAAAATCAATAACAAAGTTCTCAATTTTATTACAATTGACCAAGCAAAAGGAGTTAGTGAATATGCTCCAGGTTTAATGGCTTTAAGCAAACTTAAAAGAGATGACCAACTTAAAAAAGATGTTAATAAATTCTTCCAATCAATTTTTGATTTAAACAATTATGTTTTTCAAATGACTTCTAAAGGTACTAGCCTAAAAGCAACCGATGAGTCAATTAATGATCAAAATATAAAGACAGATAATTCGAATTTACGTCAAATTCATTTTGTTCCTCTTGCAAGTTGAAGTGATGAAGAAATCGAAAGATTGCCTAAAGTTATTGACTGATTAAATTATTGTTTCAACGATGAAGCTTCTTTTGATTTACTTAACAATAGTTACAATTATCCAACAGGCGAAACTATTAAATACTACTTTAAAGGTGTATATAGTAATGATCCTTCAAAAGCAACATGAAATACCAAAGTATTAAAATATGGCAATGTTTATTATTACAAAAAGATGAATTTTGGTGGACCAGATAGTTGGTCTCCTGCAACAACAACATATGCAGAATCACTAGAAAACAAATTTGATTTTAAAACAATGTTCTCATGAAATATTTTTAAATGATCTCAAACTTTAAGAACATTATCGAAAATAGATTTAGATTCTAAATTTGAAGTTAGTGACAGCAAAGTAGAAGACTTTACAAAAATCAAAAAAATAAAAAATAAAGATATTCAATTCAAATTAAATGTTGAAAATGAAGTTGTTAGAAATAAAATTAAATCGATTTTTTACAAATTGATTTCTCTTGAAAATGAGATATCAAACACAAAAATCGACGAATCAAAAGTATTAGGTTTCTCAACTGATTCAGCCAACAAAAATCAAATTAATGACAACACAATTAAAAGATTCCAATTGTATTTTGACAACTTACTTGATACAATTATGGGCTAATTTGGTAAATTACTTGCAAAAAGTTGCAAGTTTTTTATTTTTCATACAAAAATTGATGACTTTAATTGTTTAAATTTTTAAAAAATTTATACAAACGTAAAAATTTAAATTTATATTTAGTATTAACATTAAAAATAGTATTATTAATATATGCAAAAATATGACGAATCAAGTATACAACAATTAAAAGGCTTAGAAGCTGTTCGAAAACGTCCAGGGATGTACATTGGAAGTACAGATGTTAATGGACTTCATCACTTAATTTGAGAAATTGTCGATAACTCAATCGATGAGGCTTTAGCTGGTTATGCAAATGAAATTAGTGTAACTTTAACTAAAAATGGATCAGTCAAAGTCATGGATAATGGTCGTGGAATTCCAGTTGGTAAAACTCCTTCAGGTAAAAGTGCAGTTGAATTAGTTTTCACTGAACTTCATGCTGGTGGAAAATTTAATGAAGGAGCTTATAAAACTAGTGGTGGACTTCATGGTGTTGGGTCTTCAGTAGTTAATGCTTTAAGCACAAAATTAATTGTCACAGTTTACCGCGACAAAAAAATCTATGAAACAATTTTTGAAAATGGTGATAATATTGTCCAAAGAACACTCGAAGTTGGAAATACAAACAAACATGGTACCATGGTTGAATTTTGACCAAACTATGAAATTTTTAAACGCGCAAAATTTAGTTATGAAACTATTGCTGAACGTCTTAGAGAAAGTTGTTTTTTAATTGGTGGATTAAGAATTCACTTCAAAGATGAAATAAGTGATCGTGAAACAGAATTTATGTATGAAGATGGTCTCCATGCTTTTGTTGATTTCATTAATGATTCAAAAAATGCACTTGGGGACATCAAAACTTTTAAAGAAACAAAAAGAGATATTGAAGTCGAATGCGGCTTCCAATACACAGATAGTTATAATGAAACAGTTTTAAGTTTTGTTAATAATGTTAAGACCAAAGATGGTGGTACACATGAAATAGGACTTAAGTCTGCTTTAACTAAAACATTTAATGAATTTGCTTTAGAAGAAAAAGCTTTAAAAGGCAAAAACACTTTTGATGGTGATGACATTAGAGAGGGTCTAACTATTATTCTTTCAGTTAAGATTCCTGAAAAATTACTTGAATTTGTTAGTCAAACAAAAGAAAAGTTAGGAACACCAGAAGCTAAAGGCGTAGTTGAAGAAATAGTTTCTAAAAACTTTAAAATTTGAATTAATGAAAATAAACCATTAGCCAAAAAAATACTTGAAAAAATTAAGAGAGCTTCAGAATCTAGAGCCGCCGCAAGAAAAGCGAGAGCAGAAGCTAGAAGTACAAAAAATGCTTTAAAAGAAAAACAAATTTTAAGTGGTAAATTAACTCCAGCTCAAAGCAAAGTGCCTGCTGAAAAAGAATTATTCTTAGTCGAAGGTGACTCTGCTGGAGGTAGTGCAAAACTTGGACGTGATCGTAAATATCAAGCAATTTTACCTTTAAGAGGAAAAGTTATTAATACTGAAAAAGCTCGTCTTTTTGAGATTTTAAAAAATGAAGAAATTGGAACAATAATTAATACAATCGGCGCTGGTGTTGGTGACGAATTTGACCTTAAAAAAGCTCAATACGGAAAAGTTGTTATTATGACCGATGCCGATACTGATGGTGCTCATATTCAAATTTTATTACTTACTTTCTTCTTTAGACACATGCGCCAATTAGTTGAAAATGGAATGGTTTATATAGCTCTTCCACCTTTATTTAAATTAACTTCAACTAGAGGTAAAAAAGAAGTTTTATATGCTTGAGATGAAAATGAATTAAGAGAACTTTTACAGTTACCTAACTTTAAAAATGCTGAAGTACAACGTTATAAAGGACTTGGTGAAATGAATGCTGATCAATTGTGAGAAACCACAATGAATCCTATAACTAGAACTCTTGTACAAGTAAAAATAGAAGATGCTGCTTTAGCTGAACGTCGTGTTTCAACACTTATGGGTGACAATGTTGAACCACGTAAAGAATGAATTAACAAGAATGTTGAATTTAGTTTAGAAGATGATTTTGAAATATAGAAAGGAGCCATTATGAATAAAAATCAAGAAAACAAATTAAATGATTTATTAAGCAAGATTATTAAAGAAAATCTTGATACTATAATGGCTGACCGTTTTAGTCGTTATTCAAAATATATTATTCAACAACGTGCTTTACCTGATGTTAGAGATGGACTTAAACCTGTTCAAAGAAGAATTCTTTATTCTATGTATGAATTAGGTTTGCAATGAAATAAACCTTTTAAAAAATCTGCTCGTGTTGTTGGTGATGTTATTGGTAAATATCACCCACATGGTGATAGTTCAATTTATGAAGCTATGGTTCGTATGGCTCAAGATTGAAAAATGGGACATACACTTTTAGAAATGCATGGGAATGTTGGATCTATTGATGATGACCCTGCAGCTGCAATGCGTTATACTGAAGTTAGATTAGCTGCTTTAGCTGACTTAGTTATTGGTGACTTAAAGAAAAATACAGTTAAGTTTGCTCCTAACTTTGATGATAGTGAAAAAGAGCCAGTTGTTTTACCTTCACTTATTCCAAATTTATTATTAAATGGGGCTAAAGGTATAGCTTCAGGTTTTGCTACTGAAATGCCACCGCACAACTTAAATGAAATATTAGATGCCTCAATTGCAAAAATTAAAAACCCTGATATTCAGCTTTCAAAATTATTTAAATATGTAAAGGGACCCGATTTTCCAACAGGTGGTGTTGTCTATGGACAACAAGGAATTTATGAAGCTTTTGAAAGAGGAAGAGGAAGAATTACTCTTGTTTCAAGATATAGAACTTTTGAAGACAAAAAAAATAAATTTATCGAAATTAATGAAATTCCTTATGGTGTTATTAAATCAAAATTAGTTCATGATATTGATTTAATTATTGCTAAAGAAAGTGTTAGTGGTCTACTTGAAATTAAGGATCAATCAGACCGAAATGGAATTAGCATTCTTATCACTTTAGATAAAAAAGCCAATGAAGAAGTTATTTTAAATTACTTGCTTCAAAAAACTGAAATGCAAATTTACTATACCTATAATAATGTGGCTATTAAAAATAATTCACCACAAACTTTAGGTTTAAATGATTTATTAAATTGCTATCTTGATCACGTTCGTGATGTCAAAAGAAAAACACTCGAATTTGATTTAGTTAAATATAAAGCTAGATTAGAAATTGTTTTAGGTTTTCTTAAAGTTGCAGAAATTACTGATGAAGTAATCGCAGTTATTAGAAAAAGTGAAAACTCAAAAGCTGGTGTTATTCAAAACTTAATGAACCACTTTGGTTTTACCTTGAATCAAGCAACAGCTATTGCTGAATTAAGACTTTACAAACTTTCCAAAACTGATAAAGAAGCTTTCTTATTAGAAAAAGAAGAATTAGAAAAACAAATCAAACATGTCGAAATGTTACTAAATGATAAAGAAGCTTTTGATCAATACTTAATTGAAACATTGCAAGAAATTAAAAAACAATTTGGCCGTCCAAGAAGAACAACTATTACTGAAGAAGCATTTAAAGTAAGTTACAATGAAGCTGACTTAGTTAAAGAAGAAGAAACTTATATTGGAGTTACAAAACATGGCTACTTAAAAAGAATGAGCAACAAAGTTGTGAACTCAAATGACTTCTTAACTTATGGAATTAAAGAAGGCGACAAAATTATTTGTTTTGAAAAAAGCAACACAATTAACAATTTATTGCTTTTCACAAATTTAGGTAATTATGCAATGGTACCTATTTATAAAATAGAAGAAAGCAAATGAAAAGATTTTGGAGTTCACTTAAGTGACTTTGTTGAATTAGTTTCAGGTGAAGAATTAGTTGGCGCAATTAATGTCACTGACTTTAACATACAAAACTATGTTTGCTTATTTACTAAAGAAGCTCAAGGAAAAAGAGTTGTTTTAAAAGAATTTGAAGTTAGTCGTCCAAACAAAACTTTTACAGCTATGAAACTTAGAAAAGGTGATGAATTAGTTGGAGCTAAACTTTCAAATGGATATAAAGATGTTTTATTAATTACCAAACGTGGTATGGCTTCTTTATATACTGAAAATGATGTTCAAATTTATGGTACCAAATCAAATGGAACTAAGAGTTGTTATATGCCACCAATGGATTCAATTGTTGCTTTTGCAATGGTTGAAGCTCATGAAACTTTAGCAATGTTAGCTAATGAAACTTATGTTAAAAGAATTCATGTAAGCAACATCACTAAATCAGGCAAAAAGAATTTAGGTAAACCAATTTTTGCTCAACTTAAAACAAAACCTTATATTGTTACAGATGCCTATCCAATGACAGTTGATGATTACATTTTTGTAACAAATGATGAAAACCAAGTTTTACTCGAAAGAATTGTTGAATATCCTATTACTTCTATTAATGAAGGATTTTCAAAAATTAAGATCAATAATTCAGCTGCATTATCATTTAAAAAGAATGAAACTTCTTCATCTTCTAAACCTGTAGAAATCAAATTTGAACAACCAGCTCCTACTGAAGAAGCTAAGTTTGAAAAATCAGAAGAAACAATTGAAGTACTTTCAAAAAGAGTTGAAGAACTCTTGAAAAAATTTGGCAAATAATACTATAAAACAGTAGTTTTAGAAAAATTGAATAATTTTAAAATGGTTCTTTATGAATCATTTTTTGATTTTATTTTATAATTTTAAAAGAGAAAATTATGGAAAAAGAAATAAAAATTTTAGTAGTCAGTGATATTCACGGGAAAAAATCAATAGCTGACAAAATCTTAAAAAATGAGGACTATGATTATGCAATAAGTTGTGGCGATCATTTACTAGATAAAGACTATATGATAAGCAATTTTGATTATTGCGTTGATGGTAATAATGATATTTATTTTGACAAATATTGCTTAAACAAGCCTACTTTAGATTTCACTTTAGCTGATTATAAATTTCATGTTGAACATGGTCATATGATCGGAGATTATTGATCATTAAGAACACCAAATGTGTTATTTGATGAAACAAAAAATATAGATTGTGACTTTTTAATTTGTGGTCACTCTCATTTTCCTATTTATTATAAAGATGTTAAAACTCATAAAATTTTGTTAAACCCTGGCTCACCTTCATTGCCACGTTGAAATTCAAAACCAAGTTATGCAATAATTACTATCAAAAATGAAAAAAAATATGAAAATGTGGAAGTTATTTTCAAAGAAGTTAACGAAAAAAGTTAGTCTTTGGTAAAATTTGGGTGATATTTATTATATTTTACTTTTCTTACAATTATTACTAACGAAAGGAACAAATGAGTAACAAAAAAAATAAACCTCAATATTTATTTGCTATTGACCTTGATGGAACACTTTTAGCATCAAGTGCAACAGGTGAAGTTCATAATACAACAGCTGAAGCAATAGCAAGAGCCAAAAAAGAAGGCCACATTGTTTGTGCTTTAACTGGTAGACCATGAAGAAGTACAAAACCAATTTATAAAGCTTTAGGACTTGATACGGTTGTAGCTAACTACAATGGTGCACAAATTCACCACCCTGAAGATGAAAACTTTATTCCTTACATCAAATATTTAGACTTAAACGAAATGCTCTATATTTTAGGAGACCCTATAGTTAAAAATGAAATTAGCAATGTTGCTATTGAGGGACCTGGTTGAGTTCAACTTCAACATAGAGATCCTGATTTAGAAAGAGTTTTTGGATTCTCAAGCAGTGCTAAATTTATCGTTGGTTTAAATTTTGAAAAAATTCCTCTTAAACCAACTGGAATTATTTTTGATGTTAAAGAAACCACAGATGTTGAAGCATTAAGAAAATACTTAAGAGCTAAATATGGTGACTTAGGTGAATTCTCATACTGATCAAAAGGTGAAGGTTTAACTCCAGTTTTTGATATTACTAATGTTTCGGTTAATAAAGGTAGAGCACTTTCATTATTAGCTAGATACTATGGAATTGATTTAGAACACACTGTAGCATTTGGTGATGGTTTCAACGATGTGCCTATGTTTAAAGTTGCTAATATTTCTGTAGCTGTTGGTAATGCACCAGATGCAGTTAAAAAATATGCAACAATCAAATTAAAGAAAACAAACCGCCAGGGAGCAGTTGGTGAATACATCAATAAATTCCTTGATAATCCTGAAAAAGAAATAGAAAAAAGTAATAAAGTTATGGCTAAATTAATTAAACGTCGTGACGAAGAAGCAGATTTGGAAGACTAATTATGAAATTTCTTGAATACAATTTTTCTTTGGATATTAAAGACAAAAAACTCATTGGAGTTGACGAAGTAGGAGTGGGCGATTATTTTGGTCCACTTTGTTGTGCTGCAGTCTTTATTCCTCAAGATAAGATTCAAGAAGTTATTGATTTAGGTGTCAAAGATAGCAAAAAATTAAGTGATAAACAAATTAAAATGATGGCTTATAAACTTAAAAAAATTGTTAAGTATGCAATTCATCATTTAAGTCCAAAAGGTTACAATACATTAAATAAAACTTACAATGGAAATTTTTTAAAAATGTTTACACATTTAAGTGCAATTAGTTCACTTCAAGAAAAAATAGGCGCTGTAGATTATGTTTTTATTGATCAATATTCAACAAAAAGCAGTATTGAAAGATATTACACAGATTTAGTTAAGCTAAATAATTGAGCTGACTTTAATCAAATTCAAGGTGATATTTTACTTGCTTATAAAGCAGAAAGTATTAGCCTTGAAGTAGCTTGTGCATCTATTATTGCAAGAGATTATTTTTTATACTTTATGCAAAAAATGAATGAGCAATATAATTGCAAATTTCCCTTTGGAGCAGGCTCAAAAGTCAAAGAATTTGCTCGTGAATTCTTCAAAAATCATCCTGACAGTGAAACAAAAAATAATGTTTGTAAAAAGAATTTTAAAATGGATTTAGAAGATCAAAACACAACAACTATTAATTTGTTTGCAACAGATTTTGAAAATAAAAAATAATTAAATTCAACTTTTCTAAAGCAAAAAATAAAAAAATATTATAATTTTTATGCAAATTAGAGACGTGCGGATCTAATTATTTTTTTATTCTTTTTTATTGATTATATATTAATCGAAATTTAATTTTTCTTTTTAGTAAGCAATATTTATTGAGTAATTATTGAGTTAATTTGTTAAAAAAAATTGAAAAATTTAGCACTTTTATGTTGTGAGTGCTAAAAATGTGTTATTATTTATACATATACTAAAACGTATTTGAAAGGAAAATTATGGCAAAAGAAACTATTATTGGTATAGACTTAGGTACAACTAACTCAGCTGTAGCTATTGTTGATGGTGGTACACCAATCGTTCTTGAAAACTACAATGGTAAAAGAACAACTCCATCTGTTGTAAGTTTCAAAGATGGCGAAATTATTGTTGGTGAAAATGCCAAAAACCAAATCGAAACAAACCCAGATACTATTGCATCTGTAAAAAGATTCATGGGTACAAAAAAAATATTTAAAGCAAATGGAAAAGAATACAAACCAGAAGAAATTTCAGCTATTATTCTTGACCACTTAAGAAAATATGCAGAAGAAAAAGTTGGACACAAAATTGAAGCTGTTATTACAGTTCCTGCTTACTTTGACAATGCACAACGTGAAGCCACAAAAATCGCAGGTAAAATTGCAGGTTTAGACGTTCTTCGTATTATTAACGAACCTACTGCTGCTGCTTTAGCATTTGGACTTGATAAAACAAACAAAGAAATGAAAGTTCTTGTATTTGACCTTGGTGGTGGTACATTCGACGTTTCAATTCTTGAATTAGCTGATGGTACATTTGAAGTTCTTGCAACTTCAGGTGACAACAAACTTGGTGGTGATGACTGAGACCATGAAATTGTAGATTGATTAGTTGCTAAAATTAAAAACGATCACAAAATTGACATTCGTGAAAACAAAATGGCAATGGCTCGTCTAAAAGCTGCTGCTGAAAAAGCAAAAATTGACTTATCTTCATCATTAGTTGCTCACATTTCATTACCATTCCTTGTTTTATTAGACAATCATGAACCTATTAACGTTGAAGCTGAATTAAAACGTAGTGAATTCGAAAAAATGACTGCAAAACTTGTTGAACGTTGCCGTAGACCAATACAAGATGCTTTAAGTGAAGCTAAACTCAAGATTTCAGACTTAGATGAAATCTTACTTGTTGGTGGTTCAACACGTATTCCTGCTGTTCAAGCTCTTGTTGAAAAAATATTAAATAGAAAACCAAATAAATCAGTTAATCCTGATGAAGTTGTTGCAATGGGTGCTGCAATTCAAGGCGCTGTTCTTGCAGGTGACATTAACGACATTCTTTTAGTTGACGTTACACCTCTTACACTTGGTATTGAAACAGCTGGTGGTATCTCAACACCTCTTATTCCAAGAAACACACGTATTCCTATTACAAAGAGTGAAACATTTACAACATTTGAAAACAACCAAACAGATGTTACAATTAAAATTGTTCAAGGTGAAAGACCAGTTGCTTCAGAAAACAAATTATTAGGTCAATTTAACTTAACAGGTATTCGTCCAGCTCCTCGTGGAATTCCACAAATCGAAGTAAGTTTCAAAATTGATGCTAACGGTATAACAACAGTTTCTGCAAAAGATAAAGATACTCAAAAAGAACAAAGTATTACTATTAAAAACTCATCAAAACTTTCTGAAGAAGAAGTTGAAAGAATGATTAAAGAAGCTGAAGAAAACCGTGAAGCTGATGCAAAACGTGCTGCAGATATAGAAATTATTGTTCGTGCTGAAACAATGGTTGCTAAATTTGAAAGTGTTTTAGAAGAAAACAAAGACAAATTAACACAAGATCAAATTAATCAAGCTCAAGCTGAAATTGACAAAATCAATGGTTTTATCAAAGAAAAAGAATATGACCAACTTCGTTTAACAATCAAAGCTTTTGAAGAATTATTAGATTCAATGAGCAATGCAGACTCATCATCATTTAAAGAAGAAGATGCTGAATAGTTAATTTAAAAATAAAAAAGTGCCAAATGCTGGTACTTTTTTATTTTTCAATAACTAAAATAAAGCTTTTATTGAATAAAAGTATACTTTTAATATATAAAATAACCAAAAAAATGAACAAAATTAATTTTTTTAATTTTTTTATGCTTTTTTGCTAGCATTTTACTCTTTTAGAATAAAATAAGAATATGAAAAATTTAGCTAATGAAATAAGACCAAGAACTTTAGATGACATTGTCGGCCAAAAGCATGTTGTCTCTTTATTAAAAGAAATAGTTAAAAATAAAGCCTCAACAAGCTTTATATTTTTTGGTGAAAGTGGAACTGGAAAAAGCTCAGCAGCAGTTGCTCTTGCTAATGACTTAAAACTTAAATATGGTTATTTTAATGCAACAATCAACAATAAAGATGAATTGGTCAATACCATTCAAACAAATGACATCATTATTATTGATGAAATTCACAGATTAAATAAAGACAAACAAGACATCTTACTTTCTTATTTAGAATATGACAAAATAATTGTTTATGCAACTACAACTGAGAATCCTTATTTTAAAGTCAATCCAGCTTTAAGAAGCAGAATGCAAATCTTGCAATTTAATAAACTTAGTGAAGAAGAAATTTTATTTGGACTTAAAAAAATCATCAAAACTTATTATCCAAAATTTAAAATCAAAGATGACATACTAAAAGTATTAATCAAAAATTCAACTGGCGATTATCGATCTTGTATTAACAATTTGCAAATTTTAACTTTACTTAATAAAGACAAAGAAGTCACTGAAGAAAGCATAAAAACTTTAATTCCAAATGTCAACTTTTATAGTGATAAAGAATCAAATGCTCACTACAACAACCTATAAGCATTTCATAAATCATTAAGAGGAAGTGATGTTGATGCAGCACTTTATTATGGAGCTTTAATCTTAAAAACAGGCGACTACATGGGTCTTTTTAGAAGATTAACAGCAGCCACATATGAAGATGTAGGTTTAGCTGATCCAACATTACCAATGCGAGTAGAAGCTGCTTTTAATGCAGTGGAAAGATTAGGTTTTCCTGAAGCTTACTTACCTTTATATTACATAATAATTTCAGTAGCAATAGCTCCTAAAAGTAACACAGTTTATAAAGCAATTCAAAAAGTTGAACCTTTTATTGAGCAAGGTAACATTTATGAAATACCAAAACATTTAAGAGATGCACACTATAAATCAGCAAGCAAATTAGGAGATGGTCTTGATTATAAATATCCGCATGATTTTCCTAATCACTGGGTAAAACAGGAGTATTTGCCAAAAAGAATAGCAAGTAAAAAGTTTTTTGAACCAGATAATAATGATTCACCAAGCTTACTAAAATATTACAATTTTATAAAAAACATTAAAGGAGATAAATAACATGAATGATATTAAATGAGAAGAAATCAAAACATTTGAAGATTTAAAATTAGCTAAAAGCAAAGTATATTCAAAGGACTCTGAAATAGTTCAATTACAAACAAAAATTAGAACAGCTTCGCCTGAAGAGAAAAAAGAAATAGGTCAAAAAATTGCAACTTTAAGAAACTTTTATGAAGAAAAATTCAAAGAAATTGAATTTAAAATTGAAGCCGATAAAATCAAAAAAATAATTGAAAGCCAATATGTTGATGTAACTAAACCAGTTGACAATCCTGGTTCATTGCACCCAATTACAATAGTAGAAAATAGACTTAGAGATTGATTTATTCAAAACGGATATTTTGAATCAAATGAAAGTGAAATTGTTAGCGATGTTTATAATTTTCAAAAATTAAACATTCCTGAAGATCACCCTGCTCGTGCTATGCACGATTCATTATATTTAAATCCAACAACTCTTTTAAGAACACACAACACTGGAATTAGTGCACTTGAACTTGAAAGAAATGCAAACAAAGAAATGAATAATTTCGCTATTGGAAAAGTTTATCGTAATGATGAAGATGATGCAACTCACTCACATCAATTTACACAATTAGACTTTGTCAGTGTTGGCAAAAGTGTAAGCTTCAACAATTTAATTTGAACACTAAAATCACTTCTTTCTTATGTATTTGAAGAAGAAGTTGAACTAAGACTTAGACCAAGTTATTTCCCTTTTACTGAACCAAGTGTTGAAGTTGATGTTTATTATAAAAATGGTTGAATCGAAGTTTTAGGTGCTGGTATGCTTCATCCAAATGTTATGGAACTTGCAGGTTATGATTCAAAAGAATTTAACGGTTTTGCTGCAGGCATTGGAATTGAACGTTTGACAATGATTAAATACAACATCAAGGATGTTAGAGAATTCTATAAAAATGACTTGAGAACTTTAAATCAATTTAACTATGAAGAATAGCTTCCAAACATTTTTAAAACAAGAAGAATCTAAAAACTATTATCAAGAATTAATAAAAAAAGTTACTCAAGAAGAACAAAAACACAATGTTTTTCCACCTAAAGAATTACGTTTTGATGCTTTAAATTATTTTGAAGCAGAAGAGACTAAATTAATAATAATTGGACAAGATCCTTATTATTTAAAAAACCAAGCAGATGGTTTAGCTTTTAGTACTCAAGGCAATATTTGTCCTAAAAGTTTAAACAATATTTTTAAAGAATTAAAGAAAGATTATTCTGAAACAAACATTGAAACTTATAGCTTAAAAAGTTGAGCAAAACAAGGAGTTTTATTAATTAATACTTGTTTAAGTGTTAATGAAAATCAACCATTAAGCCATAAGAATTTTGGCTGAGAAATATTTGTTATTAATTTAATTAAATACATTTTGAAAAAAAATAAAAATGTCATATTTGGCATCTGAGGAAATGATGCAAAAAGAATATTGCAAATTGTTCAAAATGACTATAAAATAGATGAAAACAAAATTATTTTTACTTCTCACCCTTCCCCTCTTGCATATTCAAAAACAACTAATAGTTTTAAAGATTCAAAGTTTTTTATAAGAGTAAATTCATTATTAAAAAATCCAATTAATTGAGATTTAAGAAAGGATTAATATGATTATTTCATTAAGAGAATTAAATAAATTTATGCCTAAAATTAAGTTAGATGAAACTATTGAAAAGGTCATTAATAATTTAGGTTATGAAGTTGAAAGCATGACTAAATTTAGTGATGTTAAAGGAATTAAGTTTGCTAGAATAATTGATGTTAAACCTAATCCAAAATCAAATGGTTTAACAATTGTTGAAGTTGAAACTGATAAAGGAAAATTAACAATTCAAACCAAAGCACAAAATGCGCAGAAGAACAAAATAGCTGTTATTTTTGTTAAAGGAAGTCAAAAAGGCGACTTTGTATTTCAAGATAAAGAAATGGCAGGCGTTGTTTCTCAAGGTATGTTTTCAGGTTATAGTGAGTTAGGTTATGATCCAAATAAATTGCCTTTTGATAAAGATGATATTATTTTGTTAAATCCTAATAGATATACAAGTTTAAAAACTGATCCTATGGAATATTTTGGCTTAAATGACTATATTATTGATATTACCACTCCAGCAAATAGAGGCGATTCAAATAGTTATTATGTTTTAGCTCAAGAAATAGCAGCCTACTATCAAACTCCTTTTAAATGATTTGATTACAAGAAAAACTACAAAACTGGAATTCACAAACAAATTCAAAAAGTTGATAATGGTGAAGCGAATGCTTTAAGTTTTCTAGAATGCAGATTCAAACACAAAGACACTAAAATCGAAGATATGTTATTTTTAGCAAAACATAATGTAGAAGCTAAAAATGTTTGAGCAATAGATGTTACAAATTTAAACTTAATTTATACTGGTGCTCCAACTCATGTTTATGATCGCAGCGAAATAGGCGAAGAATTAACTTGCAAAAAATATAGTGGCAAAGTTACTATTTTAGGTAATAAAGAAATTGAAGTAAAAAATGCTTTAGCTATTTATTCAAAAGATAAAGTAGTTTCATTAGCTTCAGTTATGGGCTTAGAAGAAACTGCAGCAAATCGTAAAAGTAAATATGTTGTTTTTGAAATTGGTAATTTTAATCCTTCTGATGTTAGACATACAGCTAAAGAAATTAAAATTGATTCAGCTTCATCATTGCAAGGCGGTAAGGTTTTAAGTACAGAATTAGTAAGAAATGGTATGGAATATTTAAAATATAAAGCTTTTGAAGATAAGCAACCTTCTTCAGTTTTAATTAATTTGCCATCAAAGACTAAGAATAAAATTTTCTTACAACAAAAGAAATTATTAGGAAAATACTCAGGATTGCAAATCAAGGATTTGGACAAAGAATTTGAAAAAGTTGAGGATCAATTGCAAAGAATTGGATTCAAAATATTTTATAATCGTCTTTCAGCTCCAACTTATAGAACTGATTTAAATTGTTATGAAGACATAATTGAAGAATACTTTAGATTCTATGGTTATGACAAATTTAAAACTGAAGCTCCTGCATTAGCTCCATTTAAAATTAACAAAAGGAATAATGATAAAAGCTTATTACAATCAATGGGTTATCAAGAAGTAAGAACATTTACTTTAACAAATATTGATGCTCTTAAATTTAATCCTTTTGAATTTAAAGAAGATTTAAAACTTCAAACTTTTGTTTCAAAAGAAAGAGAAGCAATTAGAAATTCAATTGTTCCTTCACTTTTAGAAGTTGCAGAATACAACCTAAAAAGAAAAATGAATCGTTTAAATTTATTTGAATGTGGAATGATTAATAATGGAAAATATGTTTACGGTTTACTATCAAATACAAAAACTTTTGAACAAGTAAAACAAGATGTAGTTAACTTTTTAAATGATAATAATTTAGTATTTAAACCTTTTAAAGATAATCAATTTATACATCCAAATGTATCAGCAAAAATTTACCAAAACAACAGTTTTATAGGTTGATTAGGAAAAGTAAACCCTTCATATAATAGTGTAGATGCTTGAGTTTGTGAAGTACATAAAGAAGTAAATAAAGGCTATAAATTATTTGAAAATTATGATTCAACACCATTTAAATCAATTGACTTAACTTTCGAATTAAAACCACATCAATCAATTGATGATAAAATTATTGAAATCAAAAAAGTAGCTTTTATTTTTGAAATTTATCAAATTGATGAATTTGCAAAACCAGAATCAAGAAATGTTACTTTAAGAATTATTGGAAGCAATGAAGAAATTCAAAAAATCAATAATGAATTCAATAATTAGGAGGCATTATGTATAACAAAATTAAATTATTGGACAAAAAAATTGAAGAAGCAATTAACAATGAAACTGATCGCCAAAATAATCATATTGAATTAATTGCATCAGAAAACTATGTTTCAGAAGATGTTTTAAAAGCAACAGGAAGCATTCTAACAAATAAATATGGTGAAGGTTATCCTTACCGTAGATATTATGGTGGCTGCGAAAATGTTGATGTTGTTGAAAATTTAGCAATCGAAAGAGCCAAAAAATTATTTAATGTTAAATTTGCTAATGTTCAACCTTATTCAGGCAGTGTTGCTAATGCAGCAGCCTTAGCTTCTTTAGCAAAAACAGGCGAAAAAATTATGGGGCTTTCACTTGCATCGGGTGGTCACTTAACTCATGGTTACAAGATTTCATTTAGTGGAATTTTATATAATAGCGTTTCATATGAAGTAGATAAAAATGGATTCTTAGATTATGAAGCAATTAAAAAAATAGCTATTAAAGAAAAACCAAAAGTAATTATTGCTGGTTACTCAGCTTATTCAAGAATTATTGATTGAGCAAAATTTAGAGAAATTGCAGATGCATGCGGTGCTTACTTGATGGCTGATATTGCGCATATTTCAGGTTTAATTATTGCAGGTGTACATCCTAGTCCAGTAGGCTATGCTGATGTTATTACCACTACAACTCATAAAACTTTAAGAGGTGCTAGAGGTGCAATTATAATGACTAACAATGAAGAGTTAGCTAAGAAAATTGATCGCTGAGTATTTCCTGGATATCAAGGTGGGCCTCTATTCCATACAATAGCAGGTAAAGCTGTGGCTTTTGGAGAAGCGTTATTGCCTTCATTTAAAGAATATGGTAAAAAAATAGTAGAAAATTCTAAAGAATTTGCACAAGCATTTTTAGATAAAAACGTTGCTATTGTTTCTGGTGGAACTGATAACCATTTATTTACAATCAATGTATATCAAAGCTATAAAATTTCAGGTAAAGATGCTGAAAGTTTGTTAAATAAATTTAACATAACAGTTAACAAAAATACAGTGCCTTTTGACACTTTAAGTCCTATGATTGCGAGCGGTATTAGATTAGGCACAGCAGCTATGACTTCAAGAAAATTTACTAAATGAAGAGAGCTAGCTGATATTATTGATACTATCTTAAAGAATCATGAAACTATTCTAAAACCAGAAAGTGAAAAGTTATTTAATAGCCTTAAAAAGAAGGTTAGAGATTTCACTAAAAAATTCCCAATCATTACTGTTTATAGATAAAGACGTCAAAAATGCATCTTTATCTTTTTATAAAAAAACTTGCTAAATAGCAAGTTTAATTTTGCATTTGAAGAACAGGCTGATCTTTATTACTATCATCCTCTTGTTCAAGTTGTTTTTTATATTCTTCTTCAAGTTTTGCGGCTTCTGCAAAGAATTTGTGTTCAAGTTTTCTACTTCAAGTAAGTTTAGCTTGATCAATTTTAGGTATAACTTTAGAGACATAAATGTAGCTTCAAATTAATAAAATTATTGAAGCGACTAAAGCATTAGCAATGAATGCTGCAACCATTATTCAGTTAGCAATATATTTGCTTCCAAATTTGTTGAAAATACCAATGGCAATACCAAACATACTTGGCATAATTACAGCAAAAGTTATAAAGTTTTCCATAATTGAAGCCATTATTGATTTACCAATATCATTTGTTCCTTGGAAAACAAGAAGTGAGCTAATTGATAAACTCAAAGCAATAACTCTTAGTGTAACTATTCTAATAAATAAAGTAGTTCCAGCAGCTCTATCTGGTTGAACATTAAGCGCAATTAATCATAAGTTATCAGCTGTAGCAGCTACAAAACTGTAAGCCAAAATTGAATAGATAACTGCAGATAACATAGTTCAAAGATAAACTTCTTTGCATCTTTTAAAGTTTCTTCGCGTATAGTTATATGACATTAAGCTTCTAGCTCCATCATTAATACCAAAAATAGAAACTAAGAATAATGAAACAATCGGCATACCTTTTGACCAATAATATTGGAATGAAAGTGGTTCCGCAAAAGGAGTTTTGGTTAAGAAAATGTTCATAACAGCAAATGAAATAGCAACACCAAATGATCTTATGAAACCACTAAAACCTAAGGCAAAAGTAGGCAACAATAATTTTTTATTAAATTTAAATTTGAATAAGTGTCCAAGCGAAAGTCAGGTTTTATTCCGCTTGCTATTAATTACCACATAAGCTAAGTAGGCAGTTAAGTTAATTGACCAACCAATAATAGTAGCAACAACACCACCTAATACACCCATTTTACATGGCACAATAAATACTCAGTCTAAAACAACATTTACAATGTTTGCTCCAATAACTCAGCCCATAACATAAGCGTTATAGCCTTCTGCTCTAATAAAGTAAGATAAAAGCATTGAAAGACCTTGAAGTATTGTTCCTGAGGCATAAATATAAACATATTGTTTTGCTCAATGAACACTAAGATGTCTTGAAGCATCATAAAATGCTTGTAGTGTTTCATTGTCTTCTTTAGTCATTAAGGTTAAATTTCTAATATTAGTTTTACCTGCAAAAGCATCAACAAGACCCGATCATATAAAGATAAATGACATTAAGGTTGCTAAGAATGATAAAGCTACTGTCATTCAAAACATAGTTGCTCACAAGTCTCTTCCACTTTTTTCTAATTTTTTAGAAATACATTTTGTGTAATAAATACTTGCACCAACAGGTACTAAAAATACTATTGAGTTAGAAAAAATAATTAAAGGTTGAATTGCAGCTGTGGTCATTGAAACAACCCCATTAGCATCAATTTTTCCTAAATTCAGATCTTTATAACTGGAGTTATTTATGATATCCAAGTATTTCTGCTTCAATTCGTTTGAAGTTAAAGAGTTTAAAGTATCTGGTAAAAATTGAATCATTTCGCTTCAAATATTTCTATTATCAGGAACAAATTTTTGTAAGAAAACTTGATCAATAAAAGTATATAAACCAAACATTAAACTACTTAATAAAGAGGGAATGGCAACTATTCAAATAGCTTTCTTTATTGGAGTATTTGCAAATAATTTTTCTGCTCTTTCCTGTTTAGTTATTTTTGACATTTCAACCTCCTTTATATGAAAAATAAAAATTCATAATGGAAAAATCATACCATTATAGTCATTCTATTAAAAATAAAATTGGTGTTTAATAAAACAAATGGTGCCGACAACTGGACTTGAACCAGCGACCCCATCCTTACCATGGATGTGCTCTACCGACTGAGCTATGACGGCTAATTATGTTTTTTATTATAACAAAAATCATAGTATTTTTAAAAAAATAATAAATATTTATTGGGTAAATTTTTAAAAATATTTGCTATGTATTTCAATATTTTTAAAATTAATATTTTTACGAAAATCACTGTTTTATAAGCTTTTTTGTAAAATTAATCAATCAAAATTTTAATTAACATTTTATATATATAAAAGTTATTTAAAGAAAATTTAGCACTCTCGATATAAATGTGCTAAAATAGAAATATGCCTAAAAAGAATTATTATGATGTGCTAGGTGTTCCAAAAACAGCTAGTGAAAGAGAAATTAAAACTGCTTACCGTAAATTAGCAATGAAATATCACCCTGATAAATTAAAAGATGGTACCAGTGATCAAAAGATGCAAGAGCTAAATGAAGCTTATGAAGTTTTAAGTGATCCAACCAAAAAATCTAATTATGATCGTTTTGGTTCACCAGATGGTCCACGACCTGGACAAGGTTTTGGAATGAACTTTGGTGGAGAAATGAGAGACTTTAGCAAGTTTACTCAAAATATTTTTGATACAGTATTCAATTTTGGAGGTGGAGCTTCAAAACAAAAATCTTCAAAAGTAGAAAAAGCAAGAGGCGATGATATTTACACTGAAATTGTAGTTGATTTCATTGAAGCTGTTAAAGGTAAAGAGTTTAAAGAAAAATTAAATAAATTTGAATTATGTAGTAGATGTAAAGGAACAGGAGCTGATAATCCAAGTGATGTTAGAACTTGTAATGTTTGTCAAGGAAAGGGACAAAAAGAAACACAAACACGTTCTGTACTAGGTTACATGAAAATGGTTTCAACATGTGATAAATGTAATGGAACAGGTAAATTAATTGGCAAAACTTGTCGTGAATGTAATGGATCTTTATATACGAAAAAATTAAAAAATGTTACTTTCAAAATTCCGGAAGGTTCAGACAGCGGCGACAAAATTAAAATCGATGGTTATGGCGAAAAAGGTCGTAATGGTGGTGAATCAGGTGACTTATACATTGTTATCATTGTTAAAGAACATAAATACTTCAAGAGAAATGGTCTAGATTTATTCTTAGAATTGCCAGTTTCTTTCTTAGATATTGTTAAAGAAAATTCAGTTTTAGTTCCAACACCATACGGATTTGAAAGAATTAAAATGAAAAATACTTACCAATTTGGTAAAGTTCTTAATTTAACAGGAAAAGGGATTAGAACTAAAAAAGGAACGGGAAATCTAAAAGTAGCTCTTCAAGTTGTTATGCCTAATTTCACAAGTGACCAATATAATGAAATGGCAAAAATTTTAGAACCATATGAAGATAATGTTAATGCAGAATTTTCAGAATTAGTTAAAAGCGAAAAATAATATAAGCCCTAGCGGCTTTTTTTATATAATTAAATCATGAAAAATGACAAATTAGTTCAAGAAAAATTAAACCAAATTAATGGCACATATGATGAAAATTCAATGTTTAAAAGTTTAATTAATAGCGATGAAGAATTGCTCAAAAATTTTAAATTGTTTTGTGAAATTTATCAAAACAACAATTTTCTTGAACAAAAATTTTTAAACCATACAGTCAATGAATTTAAGAGAAATAAAAATGAAGAAATTGATTTATTTGACTTAAAAAAATGAATCGATTTAAAAAATAAAGTTGATTCATATGACACTCAAAATTTTGATTTATCAAACAATTTTTTCAAGAAAATGTCTTTAAAATCAGGAGAATTGTCATTTCTTTATGATTCATTTATTAATGAAAATGATGAATTTGGCGAATTTGATTTTGGAGAGGAATCAAGATTCAATAATAATGAAACTTCAGTCATTGCAAAAACAACAGAAGTTGTAGTTAACAAAGTTTTAAAACTATAACAAAAGTTGTTTTTTTATTTTTCTTTTATATATAAAAGCCTTTTGATTTTTAAGGAAAAAAGTTGCTTTTGTAATTAAATTTAAGTATAATACTAAATATTAAAATATAATTAGAACTATGATAGGTGTTGATTTAGTTAAAATTTCCAGATTTGAAAAAGCAAAAATTGATTTTGTTAAAAAGTTTTTACATCCAAACGAACTGGAAAAATATTTGCTGATAAAAGACAATTATTTGAAAAGTAAATTTTTAGCAGCAATTTGAGCAATAAAAGAAGCAATGTTTAAAGTTGACAATTCTTTGTTCGAATTTAAAAACATTAAACTTCAAAAAGAAAACGAAAGTTGACAACATAAAAATTTTGATATTTCAATTTCACATGAAGATGACTATTTAGTAGCTGTGGTTTTGAAAAAGAAGGAGTAATATGAATTATCATTTAAAAATGTTTCTTACATGGTGAATATGATTATGAAGGATTTATCGGATTAGTGTTTATGCGCGTAAATATCGTCATACTCCTGATTACTATACACCACAACAAAGATACGATTGATTACTTAAAAAAGTTAAAAAATATTTAAAGCTATACAACATTAAAGTTACTGTTGAAGGTTATGACAATTTACCTAAAGCGCCTTGTTTATTGATTCCAAACCACAAATCAAATGCTGATGCTTTGATCTTAATGGAAGCTTTATCTAAGAAAACCGCATCAAAAGAAGAAATGAACAAAATAGCAACATTTTTAGCTAAAAAAGAATTATCTAAAAAAGCTGTTGTTAAAAGTGCACTTAATTTAATTGACTCAATTTACATTGATAGAAATAACTTTAGAGATGCTTTTGAAAGCTTAACTAAATTTGGAACTTTTATTAAAGAAAATAGAACTTATGGTGTTATCTTTCCTGAAGGTACAAGAGTTGAAGAAGATGGCTTAGGTGAATTTAAATCAGGTGCTTTCAAAGTTGCAATCAGTCATTATTTACCAATAGTACCTGTAGTTATTAGTGACACAAGAAAAGCACTTAACAAAAACAGATGAAAGAAACAAGAATTCAAAGTTAAGTTCTTGAATCCAATTAAACCAAGTACATTTATGTCAATGGACCCAGGAGCTTTAGCAGATAGAGTAAAAAGTCTTATGGAAAGGGAGCTAGAAAATGAAAGCTAGTTTATATGCAACAGATGATCATAAATATGACATTAAGTTAGAAAACTTTGATGGACCACTTGATCTTCTATTAGCACTAGTTCAAGAAAAAAATGTTGATATCATGGATGTTGACGTTAGTGAACTTGCTACAGCTTACTTGAAAATTATTCAAAATTTACAAGAGCATGAAATTGATATTGCTGGTGATTATTTAGTTATGGCTGCTACACTTTTAGCTCTTAAAACTAAAATGCTTCTCTTTACTCCAGAAGAAAAACCAGAAATTGAAGAAGACAAACGAGAAATCTTACGCCGTCTTTATGAATACCAACAATTCAAAGAAATTTCACAAACACTTAGACAACAAGAAGCTTTACGTCATGAAATCTTTATTAAGAGTCCAAGTGATGTTGAAGAATTTATGGTTGATGATGACTCAACTGCACTTGATGGAGCTTCAAATCCACTTAAGTTAATAACAACACTTAGAAAAATGTTTGAGCGTACTTACGCAATGCAGCTTAGAAGAACTAAGCTTGAAACATTTAACTTAACACCTAAGGATCAAATACCTTTTATCTTAAACTTATTTGACCACTGTGACACAGTCACATTCGAAATGATTTTTAAACAACCATCATTAAATCACTTCGTTATCACACTTTTAGCGGTTTTAGACTTATCTAGAAGACAAATTATTATATTAAAACAAAATGAACAATTTGGCACAATTACATTTGAAAAGGGGCCTGAATATGAAAAATAAAATATTAGAAGCTTTACTATATGTTCAAGGAGATGAAGGATTAACATTAATCCAAGTTAAAGAAATCTTTGGACTTAACACAATTGCAGAAGCTAGAAAAGTTATGAATGATTTTATTAAATCATACAATGATGCTGATGGTGCATTAAAAATAGTTAACTTCAATGAAGTTTATAAATTAGCTACTCGTGAAACATATAAAGAATACATTACCAAAATGGTGCAAGTAGTTAAAAAACACCGCTTATCAAATGCTGCTATTGAAGTTGCTGGAATTACAGCTTATAAACAACCAGTTACACGTTCAATGATTAACAACATTCGTGGTGTTCAAAGTGATCAAGTTTTAAACTCACTTTTAACAAAAGGTGTTATTGAAGAAGTCGGAATTAGCCCTACACCAGGTAACCCAATTCTTTATGGAATTACAAACAAGTTTTATGATTACTTTAAAATTAAATCATTAGCTGAATTACCAAAATTAACAGAATTTAACTTTGTTGATGGTGTTGAAAATGAAGCTGAAGACTTTAACTTATTTGATAGCCAAAGACCAGAAAACTATGATAATTAATTTAATTAAGGGTGCTATTTTTAGCACCTTTATTAATATTTAAGAAAGGCAAAATGCTTGAATTTATTGCAACCAAAAATGATGATGGCCGTAAATTAATTAGCTATTTAAAACGAATATTAATTAATACGCCAACATCACATATTTACAAAATTTTGCGTAATAAAGATATTAAAATTAATGGTGTTAGAATCAATGATCCTCAATACATAATTAATGAAAAAGATATCATACAAATTTATGGAATTGAGGCTAATGATTACCAAAATAAGAAGTTTGAAAATAGTGCAAAAAAAGATTTTAAAATCATTTATGAAGATGACAATATTTTGATTGTTTCTAAGCCAATAAACTTAGCTGTTCATTCAGAAAATAATTGTCTAGATAAACAAGTTTTAAGTTACTTAAAATATAATCAAAAAGATAGTTTTAAACCAAGTCATGTTGGACGTCTAGACAAAGTGACAAGTGGATTAATGATATATGCAAAAAATTATGCTACTCTTGTCGAATTAAACTATAAAACAGGTTTTTTTGAAAAAATATATAAATTAAAAAGTGATTTTAATGAAGTTAAAAAACTTGTAGAAATTAATATTCAGCATGATGAAAAATTACACAAAATGGTTGTTACAAATAATGCTAAAGATCCTTTAGCTAAAACACTTTTTTATACAAAAAATAATGAAATTTTTGCATCTATTTTAACTGGCAAAAAACATCAAATTAGATTAAGCATGTCTCATTTAAAAACACCTATTTATGGAGACATAAAATATGACGGTAAAAAAGCTGACAGATTATATTTGCATTGTTATTCAATAACATTTAAAAATTTAGATAATAGTTTAAAATATTTAAATAATAAAGAATTTAATGACCCTATTAATTGATAAGGAGAATTATGAAGAAAGTTACAAAGGAAGAACTTAAAAAAATAGTTGCTTCATTAATGCTTGAACCAACTAATGAAGTTTTAGACCAAATTATGACTGAATGAAAAGTTTTGAACAAGAACTTAAATATGCTTAAAAAATTAAATACAACAAATGTTAAACCATTAAGTCACATCAATGAAGAAGCTTTAGTAGACTTCTTAAGAGAAGATGTTGAAGACACTTCTTATAGTATCAACAAACAAACTGCATTAAACAATGCTATGGACAAAGATCAAGATTACATTATTACAACAAAGGTGGTGAAATAATGAAACTACAAGTCTTAGGTGATTTTGATTTAGCCCAAGCTGAATTAAAGAAAAACAAAAATAATGCAGTTGCTTATGTTTATGATAAAGCTTTAAAAGAAGGCGATGGTTTATTAAACAATGCAGTCTTTACAATTAAAGAAGTTTTTGCAACTAAAGATGCAAAAACAACAGCTTCGAGCTTAATTTTAGAAAACTTCAAACCTGGCTACAATGCAACCTGTGTGCAAAAATTAATTGATGCAGGTGCTTTAAGAGTTGCAAAAGTTTATTGTGATGAATTGGCTCTTGGTGGCACTGGTACTTTTAGTGCTTTTGGTTTAATTAAAAATCCACTAGATAAAGAAAGATTAGCGGGTGGTTCATCTTCAGGATCTGTAGCTACATTTACAGATAATATTTCTTTTGCACTTGGTTCAGATACTGGTGATAGTGTTAGACTGCCAGCGAGTTATAATGGAAATGTTGGATTTAAACCAAGTTATGGAGCAATTAGTCGTTATGGTATGTTTGCTTATGCTTCAAGCTTAGATACAGTTAGCTACTTTGCACACAATGTTAATGATATTGCAGTGATTTCGCAAGTTTTATTTGGAGTAGACAACAAAGATTTTACTTCTAAAAAAGTTAAAATTGATAATGTCCAAAAACTTCAACCTAAGAAAATTGGAGTTTTGAATGTAAGCAAGAATTTAAGCAACTTTGTTTCAAACAACTTTCAAGAATTAATAGTTAAATTAAAAGCAAATAAAAATATTAAAGTTGAAGTTATTGAGCCTCAAGAAGATTTACTCAATGCAGTTAAAGCAATCTATGACATAATTTCTTATTCAGAAGCGAGCTCAAATTTAGCAAATTTAAATGGTATTGCTTTTGGTTCAAGAAAAGAAGGCGAAAGCTGAGAAGAAATTATGACTAACACAAGAAGTCAAGGTTTTGGAAAAATGGTTCAAAGAAGACTTACTTTAGGCACTTTCTTCTTACATTCAGAAAATCAAAAAGATTTATTTTTAAAAGCTCAACAAGGCCGTAGATTATTTAAAGAATATTGAGATAAATTGCACAAACAATATGACATTGTGATTTATCCAGCTTATGCTGATGTTGCTCCTTATATTAATAAGAAGAATAAAAACTACAAATATATGGAATACATTTTAACTGGATCTAACTTAGCTGGGAATCCATCAATTACAATCCCATGAATTAAAAAAGATAACTTACCAATTAATTTAGCTATTGATGCCGATATTTATCAAGACGAAAAACTTCTAAGTCATGCTCTTTGAATGGAAGAGTTTTTAGGAGGTGAACATGAATAATTTTGAAGTAATTATTGGAATTGAAATTCACCTTGAGTTAAATACAAAAACTAAAATGTTTTCGCCAGCAAGAATAGATTTTAATGCTGAACCTAATACAACAATGAACCAAATCGATTTAGGTTATCCAGGAACTTTACCTTTAGTTAATAAGGAAGCAGTTTTAAGTGGAATTAAATTAGCCAAAGCATTAAATATGAAAATAGATGACGAATTGCACTTTGATCGTAAAAATTACTTTTATCCTGACTTACCTAAAGGCTACCAAATTACTCAATTTTTAAGACCAATAGGATCAGAAGGTTATGTCACAATTAACACTGATAAAGGACCTAAAAAAATCCAAATTGAAAGAATTCACCTTGAAGAAGATACAGCTCGTCAACACCATGATGAAGAAGGGACTAAATTAGATTACAACCGTGCAGGTGTGCCTTTAATTGAAATAGTTACTCATCCTGTTTTAAGAAGTGCAGATGAAGCCGTAGCTTATGTTGATATGATTCGTAAGATAGCTTTAAGCTTAAATATTAGTGATGCTAAAATGGAACAAGGTAGTTTACGTGCTGATGTTAATGTGTCACTTAGACCATATGGTTACAAAGGTTTTGGTACAAAAGTTGAAATCAAAAACATGAACTCATTTAGAGCAATCAAGAATGCTCTTGAATTTGAAATTGATTTTCAAAGAAAACAAATTTTGACCAATAAGCCTATTTTACAGCAAACTAAGCGTTATAATGAAGCTACTTTAAGTACAGTTGTAATGCGTACAAAAACAGGTGAAGTTGATTATAAATACTTCCCAGAGCCAAATATTCCATTTATTAAATTAAGCAAAAAATTAATTGATTCAGTTAAATTAAATGAACTTCCTTGAGAAAGAGAAGCTAGATACAAAAAAGAAAATATTCAAGATATTTACATCAACAGTTTATTAAATGATCTTGATTTAGCAAAATATTTTGATTCTATTAATTATGTTGATAGAGATAAATTATCTAAATTATTTTTTGCTGAAATAGTTTCACTAGCAAATAGCAAAAGCATTCATCCAACTGAATTAAATATTAAAAATGGACACTTATTAAAAGGAATTGAATTAATGGACCAAGATATTATTTCAGGTCGTTCATTTAAAAAACTTGTTCCTTTATTAGTTAATTTTGATGGTGATTTTGACAAATTAGTTGTTGATAATAATCTTAAACAAATTAGCGATGAAGCCACAATTACAAAATGAGTTAATGAAATTATTAGCCAAAATGAAAGTGTTATTGCAGAATATCCTAATCGTGAAGAAAGAGTTCTTAAATTTGTTTTAGGTGCTTTAATGAAAGTTTCAGGCGGACAAGTAAGTCCAATTAAAGCAAATGAAATTTCAGTTAGAATTCTTAAAAACAAATTTAAATAATCAATATAACAACCTTTTGGATGTTTATTAACAATCAAAAGGTGTTTTTTTAATTGTTTTGTGTGTTTCACACATAAAAATGTTATAATGTATGTGTAAAAATCACAAAAACACAAAAAGGAGAAGAAAATGCAAGAAGAAAAAGTTATTAAAATTGGAAAAAAACACAATGGTGTAATTACAACAAAGGAGTGCGCAATGCAAAGAATTCACAATCAAACATTACACAGGTTATTGTCAAAAAAAATTATTGAAAAAATTTCCCATGGTATTTATTTATTGACCGAGTATTTAGAAGATGATCATTATTTGTTTCAATTAAGATACCCAAAGACAATTTTTTCATATGAAACAGCCTTATATTTAATTCACGAAACAGACTTTTTACCTATAAATTTTGATGTTTCTGTAACAAAAGGGTACAAATTCAATGATAAAGAAAATATAAGAGTTAATTATGTTTCTGATGAGTTAGCTGATTTAGGAAAAATAAAAGTTAAAACAATGTTTAATAATGATGTTTATGTTTATTGTTATGAAAGAGTATTGTGCGATTTAATAAAAAATAGAAAAAAAGCAAATTTAGAAACTTATGTTAATGCAATTAAAAGATATGCACAATATAAAAATAAAAATTTGGATTTATTGTATGAAATAGCTAAGAAAATGAATATTTTAGATGAAATGAAAAATATTATGATCGGAATAATTTATTAATATGACAATAGAAAAAATTAAAATGATAAGCAAGGAAATAAGCAAAAAAACTAACATACCATTTAATAAAATATTTCATAATTATTTTCTTGAATTAATTTTAAGAAAAATATCTAAAAGCAAATATTCAAATTTTTTTGTATTAAAAGGCGGATACTTAATAACAACAATTTTTAACTATAAATGAAGAACAACAGTTGATTTAGATTTTATGTCAATTTTTAAAAACAAAAATTTAGGTGAAATAAAAGACATTGTTAATAAAATTTTGAATGAAAAAGATGAAAATGACACTATTTACAAAATATATAAAACATAACCAATAAATGTTAATAATGAAAAGAAAGGAATTAGATTGTATGTAAAAGTAAAAATAAAAAACATAACAGAAATTGTTCCTTTAGACATTGTTGAAGAATCAAAAATTGATCCTACTTATATTTCAAAAGAATTTAATTCTATTTATGGTGAAAATTTTACTTTTCTAATTTATAACATTGAAAATGTAATTGCTGAAAAGGTTTTTGCTATTTATACCAACCGTTTTTGAAATTCAAGATTAAAAGACTTTTTTGATATTTATTATTTATTTGAAAATCAAAAAAAATTTATTAATAAAGAAATCTTAAAAAAGGCCATTGACAATGCTTTTAAAGTTAGAAATGCTTCATTTGATAAAACTATTTTTATAGATTTACTAAATGCACTTAAAATTTCAGAATTACAAAATTTACAATGAGAGAAACTTGTTTTATCTAAAAATTGACAAACATCAAAAAGCTTTGGTGAAATTGTAGAAGTAATAATTAAACATTTTGAGAATGATATTGAGAATGTTTAGAATTTTGTGTAAAGTATTTCACTTTCTTAATAGAAAGGAATTGATTCAACACAAAATTTTTTATAGCCCCCCCATTTTTTAAATTCTAGAATTTAGGGTTTGTTTATTATTGAATTATTTAAGATTTTTAAGGACTACATTTTTTAGATCATTGATGCTATAAAGATGTTCGATGATTTCGAAAGCTTCTTTCAAATTGTTTTTTGCATTTTTTCAACCATTTATTCCATCGGTAATTCAAATGAATTCAAAATTAGTAATTGTTTTTGTAGATAAATAAATTTTTTCATAGCTTCTTGAAACTTCATTAAGTTTAGAACCTTGAGCCGTATAAAAATTTACTTCAATTGCATAAAATTTATGATTTTTAATGATTCCAAAATCAAATCTTTTATTTGCAGTTGTTTCTTGAATATTATTTAAATTAATTCCAAACTTTTCATAGATCTCACTTGAATATATTTGTTTAAAATATGTTTTGTTTTCAACATATCCAGCTTGTTGAATATATTTTTCAACTAGTTTTTCCATTTTAAAACCTGTTCTGTTTTTTCTTGCATTGGTGTTGCTGCCTGCTTGAACACCATCATAATAACCTAAAACATAATCTTGACTTTTATTTTTAAGTCAATCTTTTAGACCTGTTTTTTCAAAAAAGTCATTGTAATCTTTAAAGTCATTTGTAAAATTCTTAAAGTCTAATTCTTTTATTTGCTGATTATCATAAATTTGCAAATAATTATTTCTTACAGCTAATAGACTTGGCAATATTTTTAAAACTTGTTTATCATTATCATATATTTTTTTAAATTTTTCAGAAATATTCTTGTCACCTACAATATTGCTGAATTTAACTAGTTCTTTTCTATAAGCTTGTAAATCTTGATTAATTTTTTCAAAATCTACATAATAGTCATAGCCAACTATTGTTGGTGTTAAATTATTAAATCACTTTTCAAAATCTCTTTTCATTTTACTCCCTATAATTTGTAATTATTACTTCTTCAACTTCTCCTCGTCCACTTCCATTTGAATTTATCATTCTTTTTGCTTTAACAACATTAATATTAAAGTCTGCATATAATTCATTAATATATTTTGTGTTGTGATTACTTAACATTACTTTGACACCTTTTTTGTCAAGTTTTTTAAAAACTTCGGATAATCGTTTTTGTTCTTCTTTTCCAAAACTTTCTGAATTATATGAAGTGAAAGTATTTTTGTTTTCAATGACATCATAAGGAGGATCAAAATAAACAAAATCTCCTTTTTGAGCTTTTTCACAAAATACTTCAAAATCTTCACAGAAAATTTGATTATTTGTAGTTTTTAAAAAGTTTTTTAAATTTTCAAAATTGTCTCTATCAAAGCAATTAACTGAGTTTTTCTTGCCAGAAGGTACATTAAATTGTCCTTTAGAATTTACTCGATATAAACCATTAAAACACGCTTTATTTAAGTATATTGTTCGGGCAGCTTTTTTGTAATTTGGCAGTTTATCGTATTTATCTAATTTGTCCAAATCTCTTATTTTATAGTAATACCCATCATTATGATTGTTTTCATGATTAATTAATTCTTTTTTTAATAATTTAAAATCTTTATCATTTTTAAAGCAATTAAAAACATTAATTAATTCTTTATTAAAATCATTTATTATGAAATTTTGCGGCTTTAGATTTAGTAATAATGCTCCGCCACCAACAAAAGGTTCCAAATAATTGTTATATGTTTTTGGCATTAAACTTTTAAGTTTATCTAATAATTGAGTTTTGCCACCAGCCCATTTTACAAATGGTGAAAGTTTTGTGTTTAATTTTCCAATATAAATATTATTCAAATTCAAAGCATTATATATTTTGCAAATTGTAAAAAACTGAACGCCCTTAATTTGACCTTTTTCAATTTTAGTTAATTGAGATCTTTCGGTGCTACTTAATTCTGCTAATTCTTGTTGTGTCATATTTTTTGCAAGTCTTTCCATTTTAATATTGTCGCCAATTGTTTTAAAAAGTGTTTCCATTTTGTGCTCCTTAAATAAATATATTTTAATTATATCAAATGTGTTGTTTTGTACACAAATTATTTCACTTTTTATTTTAAAATTTTTCAATATCCTCTTGTCTTACCAATTCTAGCTATGTAGCCCTTGTCTTTTAAAAGATCTAAAATTCTTTGAATAGTTCTCGAAGATTTTTTTATTTTTTTAGCTAGTGAACCAGCTGTTTCTTTTGGATTATTTTTTAAAGCCATATATACATAATAAGCAGAATCTGGCAATCAGTATTCTTTAAATTCTTCATCTTTACTAATTAAACTTTTGTTTTCTCTTAAAAATATAAATGCAAAACCATAATTGCCTTTTTGATAAGTAATTTTAGCATTAGTTTTTTCACATTCTTCATATACTCTCTTGAAACCATTATTACAAATTTTAGCTTCATCAACTAAATTAATCATTTTTAAAATTAATGGATTTCTAATAATTGATTCTATGTTTTTATATGCAAAATCTTCTGGAGTGTATTCAATTGGAAATTGACCAGGATTATAGATTTTAATATTACTAGGATGAATATTAATTTCGTGTTCTATTGCAGATTCATATTTTGCATGTGCAAAACTATTAATAATTATTTCCTTTAAAGAATCAATTGGTATTTCTGGAATATCAATTCTTTCTATATCTACAAATTCTGCTTTTCAATTGATTTTAGATTTAACATATTCATTAGCTTTCTCAATTAATTGAAAGATGTTACCAGAAATTCTATTGATATCTAAAAATGTTAATTTTTGATCTGTTGCAAAAACAGCCATTTTTAAAACTATAGGTTCTCTATGCGAAAAAAGATAATGAGCAGCATTTGTTAGTTTTTCATTTTTCATCAAACTTAATTTATTTAAAATGTTTTCAGCATTAAATTCTATTTCATTTAATTTTTTACTTTTTATAGCTTTTTTATAAAACTTTTGAAATGTTTCTAAATCAATATCATCAATTTCATAATCGCTTAATTCACTATCTCAGCTTTTGTTTTTATTGAATTCAAAAAGCTGTCTTAATTGATTAGGACTGAGTATTTTATCCTCATCATCATTTCTAATATAATAGATGCCTTCAACTGAATATGGAGCATCATTCCCACAAAAAGTTACCTCTATTATTTGTTTATTATCAACAATTTTTTTCTCAATAATAGGACTAATTACAGGTTCAATATTTTCATAAATTTTTCTGCAAATATCTCTTAAAGTATTTATGTTGATTTTTTGATCATATACTAATCCATCATCTCCGATGCCAAAATATAATGTGCCTTTTCCGTGTTTATTTAACATGGCACAAATTGATTTCATAGCCTCATTTATTTCATTTGTATTTTTCTTAAATTCAAGTGTTTCGCTTTCGATTCCAAAATTCATTTTTAACCTTTTTTTAACTTTTATAATTACATTATACATAAAAAATTTATTTAAGACAAATTTATGACAAAATTTATGACAAAAAAATAAAAAAATATTTGTTCTTATTGTGATAAAATTAAAATAATTAACATAAAAAGGGACAAAAATGAAACAATACAAAAACATAAAAGAAGTTGAAGAAAAATACACTTTTGATCTTGAAGATATCTTAAAAGGGCAAACAATTTATGATTTATTTAATGAGTTTATTGAGCTTTCTAAAATAGCTTTAAAAACAAAAGACACAAAATATGAAAATGTCGAAAACTACATTAAATCAGTTGAATTGGGAAAAGATATGGGAGTTTTATCTAATAGAATTCACAATTATCTTTCTAATCATAGTAATCAAAATTTAGTAGATTCTAAATGGACTGATTTAAGCACAAAATGAGAAAATATAACAAATAAAATTAGCGAAGAAATGGGTAGTGAAGCTGTTAGATTTTTTAAAAATATTGAAAAACTAAAAATATGAAAAGATGATCCTAGATTAAAAAAAGAACGACGTGGAATTATTGATGCTATTGAAGAATATGAACACAAATTAAGTGATGAAGTTGAAGAATACTTGAATCAAAGTTCAAGTGCAAATCCTGACTATGAATCTATTTTTAATATTATTTCAGATAGCGAATTAGACTATGGTTTTGCAACTGATTCAAAAGGAAAAAAATATAAATTGTCACCAGCAATTAAAGCAAAATTTATGAAAAAAGATGATTTTAAAATTAGAAAATCAACACGTGATTCATTTTTAAAAGCGCACATGAAACATAAAGATTCAATGGCTAACTTACTTTATCAACATTTCAAAGGATTAACTGTAACTGCAAAAATACGTAAATACAAAAATACTATTGATATGTTAACTCACAGTGATAAAGTAGATGATTCTATGTTGCAATTTTTATTTGACAAAGTGTCGACATTAAAACATACAATAAAAAATAGAAACAAGTATTACAAAAAGTTTTATGAAGCAAAATTTAAAGAAAAATATCATGCAAAATATGATTCATATCGTGAATTAGTCAAAGTTAAAAGTACTTACACAGTTGAACAAATGCAAAATATTGTTTCAGAAGCTCTTAAACCTTTTGGATCTGAATACCACAAAATGATTACTAAAGCAATTAATGAAAGATGAGTTGACTATATGACTGTTGATAACAAACTTTCAGGAGCTTATAGCATCGGCAATACTTATGGTTTGGATAAGAAATATATTTTAATGAATTTTGATGGTGAATTAGGTTCAGTTGAAACTTTAGCTCATGAATTAGGCCACTCAATGCATTCTTATTTTTCAGATAAAAATAATGATATAAGTAATGCTTCTTATCCAATTATTTTGGCTGAAATAGCTTCAATATTTAATGAACTTATGCTTTATAACTATTTATTAAAAACAAGTAAAAATGATTTATTTAAATTTAAAATTTTGGACAACATGATTGACGGGTTTGTTGGAACCGTATTTAGACAAATACTTTGAGCTAACTACGAATATGATTTATATAATGCTATCGAAAAAGATCAAGCTAGTCCCTCATATACTTCATTAAGTAAGATTTATCATAAGAATTCTATGAAATATGCAACCAAGAAAATTAAATACAAAAAAGAAAAAAATATTATGTCGGTTTATGTACCTCATTATTACTACGGATTTTACGTATACAAGTATGCTATAGGTCAATTGGTTGCAAATTACTTTTACACAAGAGTCAAAAATGAAGGCGAAAAATACTTGCAAGTTTACATCAATGATTTCTTAAGTGCTGGAGATAGAGATTATCCTCTTGAAACACTTAAAAAAGTTGGTGCTGACTTAAAAGATCCTCAATTTTATGAAATTGGTTTTAGTTATTTTAAAGAAATAGTTAAAGAATGAATTAAACTTGGAAAAAAGATATTTAAAGTTAAATAATTTAGTGAGCAAAACATGCTCATTTTTTATTCAAAATTATTGAAAAAATCAAATTTAACATTTTTTATATCGAAAAGTGTAAATTTTGTATTTTTACAACTCAAAATGTTGAAGCAAAAGACAAAATAACTTATTATCCAATTTACATGTCGATGTTTTTAAATGATCAAAATATAAAACTTCCTAAAAATGAAAAACATCAAGATTTAGGCGTTTTAACCCAATATTTATGATTTTTTATTTGAAATATGTTGCATATTTTCTCAATAAAGCTCATTATAAGCAATTTATCTTATAATTATTTTTATGAATTTAATATTTGCATTTAGAAATCAAAAAAGATATAAAAGAGATTTTGCTGATTTTATATTGAAAAAATATAAAAAAGCGACAAAAGCTGCTTTTTTACCTTTTCTTATTTCAACTTTAGCATTTTATTTAGCTTGATTTTTGGGATCTGCCACTGTGCCTTATTTAGGTTACATTCCTATTGGGCATACTCAAATAACCTTTTTAGCTTTTATAGTTGTTTTTGGAACTTTTCACTTAGATTATGTTGGTAATCTTTCAGCAGGATTATGTTTTGGATTTAGCTCATGAATTTTAGCTTTTATAATGGGGGCGATTAAATATCAAAACTTTGATATCGCCGTTATTCCTAGAGTTTTAATGGCAATATGTGTTTATATGCTGTATGAAATTTTTAATGTTAAGAACAATATAAAATGTTGAAAATTTATTATGATTTCAATTATTGCAGCAATTTTAAATATTGTTTTAACATTGTCATTTCAACATTTACATAATCAAATATTTGGTGATTTAAAAAATTTATTACCTATTAAAGAATGAATAATTGTTCATCCAATTAATATTATTGGCGAACCTATAATTTGTGGAATGTTAGCCTATTTCACTTTTCCCTTAATAGTATTTTTAAGAAGAAGATTCGAACAAAGATTCAGATATTATTGATAAAAAATATTAAAATATAGATCTAAAATATCGTGAAAAACGGTATTTTTTTAAATACCTAAATTTTTAAACTAGTAAAAAAATGTTTATTAAATTTAATTAATATATTAAAATTAAAATACATTATTAGTTAACAAATAAAATTTAAGGAGAATTATGGCTGAAAATAACAAGAAGTTTACTATTGATGAAAAAAAGAGTGAATTAGTTTACACTCTTGAAATTAATGGCAAAGAATGAGAAAAACTTTACGCTGATGCAAAAGCCAAGGAAGTTAAAAACATTAAAATTCCTGGATTTAGACCAGGTAAAGCACCACAACATGAAATTGAAAAAAGAGTTAATCCTCTTTCTGTAGCAAGTGAAGTAGTTGATCAATACTGAAGAAATAATCACGATCGCTTGAAAGAAGAATTATTAAAAGAAAACAAAAGAATAGCTTTCATTCCTAAAATAGATCTTAAAGATTTAGATCCTAAAAAAGGTGCAACATTAGAAATTACATTTCCTCTTTTACCTGATTTTGAAAAAATTCAAATCAAATCACCTAAAACAAAATTTGAATTAATCAAAATTGAAAAGAAAGATATTGAAGATTGAATCGCTCAACAATTAGAAAAGAATGCTTTATTATTACCACTTAAAAAAGATGAAAAAACAAAAGATGGTGACACTGTAACAATCGATTACAAAGGTTTTATTAAAGATGAACCATTTGATGGTGGTGAAGCTCAAGGTTTTGATCTTAAATTAGGATCACACACATTTATTGATACATTTGAAGATCAATTAATTGGCAAAAAAATTGGTTGAAAAGGTGAAGTTAAAGTTACTTTCCCTAAAGAATATGCAGTTGAAAAACTTAAAGGACAACCCGCTACTTTTGAAGTAGAAATTAAAGATGCAAAAAGAAGAGAAAAAGTTGAATTAACTGACAAAAACTTTGGTACAGTTCGTCTAGCAAAAATGTCAGAAGCTAAAAATGCTGCTGAAGTTAAAAAAGAAGTTCAATTACTTTTAGAAATTGGTGCTTTAGATGAATCTATTGAAAACTATTTTAATGAATTAGTTGAAGAATTTGACAGGAAAAATGATTTTATTATTCATAAAGCATTTATTAAAGAAGATGTTAAAAAATCACTTTCAGAATTAGAAAGTAATTTAAAACAACAAAAAATCAAATTACATGAATATCTTGAATTATTAGGTCAAACAAAAGAACAATTAATGGATTTATTAGCTAAAGACCACTCATCAAAAGTTAAAAAACAAACTATTTCAACTGAAATCGTTAAACAAGTTAAACCTGAATTTCATAAAGATAAATTTGCATTTAGAATTTTATCATTAGCCTATAATACAGGCTTAGACACCAAATTTATTGAAGACACATTTTTTGATGAAAAAGGTGAAATTAAATCAAGTGTTAATGCAATGGACACATTAAAAACATTCATTATTATTGAAAAAGTTTTAGAAAAATTTGACAAAAACGGTTTTGCGACATACTTAAAATCTAAAGAAGCTTTCTCAAAAGAATTAGATAAAAGAGTTAAAGCATATACTAAAAAAAGAGAAGAAGAATTAGCTAAAGGTGAAAAAGAAGCTAAAAAAGAAGAATCTAAACCGCAACCAACAAAAAAAGCTGCTGCCAAAAAAACAACAAAAAATAAAAACTAATAATTACATATAGGCACATAATAATTAATCAGGCAATTTGCTTGATTTTTTTATTAAACTTTAATTACTTATTTACTAATAACAATATTTATAATAAAATTAATTTATTAATTTAAGGAGAAAAAAATGCTTAATATTGTTTTATTTGAACCAGAAATATCACCTAACACAGGAAATATAATTAGAACCTGTTTTGCACTTGGAGCAAAACTTCATATTATTAAACCAATTGGTTTTGATTTAGATCCTAAGTATTTATCGCGTCCTGCAGCAGGCAGATTATTAAGTGATATTCAACATGAAGTGCACGCTTCGTATAAAGATTTTTATGAAAAATATAAAAACAAAAATATCTTTTATTTAACTCGTTATGGTTTAAAAACTTATACAGATGTTGATTTTAAAAAAGAATACAAAAAGAATAAAGAAGTTTGAATAATGTTTGGAAAAGAATCAACTGGAATTGACAAAGAAATTTTGCAAACTCATTTAGAAAATTGCTTAAGAATACCTATGATGAGTGAAATGAGATCAATCAATTTAGCCAATACTGTTGCAATAGTAGGTTTTGAAATTATGCGTCAATTAGATTTTAAAGAGTTAAGTCTATATGAAGTTCAAAAAGGAAAGGACTTCTTAATCAAATGGAAAAAACAGTAATTTCAAGTGTAAGTAATCCTAAAATTAAGTATTTAAAAGAGGTTCAAAAAGACAATAAAAGTGATTACTTTTTAGTATCTGGTTACCATTTAGTACAAGAAGCTTTAGCTAAAAATTTAGTTATTGAAATTTTTGAAGAAGAAAGCAAAAGCACCTATAAAACAGCTACTTTGGTAAAAAGTAATGTTTTAAAAAGTATCTCAAGTACAATGCATCCAGATGGTGTTGTAGCCTTGTGTTTAAAACCTAAAAGTCAAATAAAAAATAAAGTCATTTTGCTTGATAATGTCCAAGATCCAGGTAATGTTGGCACTATTATTAGAAATGCTGCATGCTTTGGTTTTGATACGGTTATTTCGAATGTAAATTTTTATAATGACAAAGTAGTTAGAGCAACTCAAGGTGCACTTTTCCAAGTTTCATTAATCAAAGAAAACAATAATGAAGATTTAATTAAAGTACTAAATAATGAAGGTTATTTTATTGTTGCCACTTCTCTCGATAAAGATTCAATTAAACTAGATGAATTAAAAATAAATAAAGAAAAAGTGGTTCTAATTTTAGGAAATGAAGGCAACGGAATCAATAAAAATTTATATAAATATTGTGACCAAAAAGTGTACATCCCTATTGAATTTGAAAGCCTAAATGTTGCGGTAGCAAGTGGAATAATTTTAAATAAAATATATAATAAATAATAAGAGGGTTTATGAAGAAAACAAATAACGAAAAATTTTTCAACAAAAATTTTAGTAGAGAATTAAATGGTTACTCAGTTCCTGAAGTAGATAATTTTATTGATCAATTGTGAAATTATGTTAAGCAATTAGAAACTGAAATTGAATCATTAGAAGAAGCTAGAAATGTTGATCGACAAAAATTAAATAACAAAATAACTGAATTGGAAAACAAACAATTTAATGAATTACTTGATACAAACTTAAGAAAGAAAATCAAAAAAAATGAATAATAATGAGCAAACTTTAATTAATTGATATCCTGGTCACATGGCTAAAGCCATGCGTGAAATTAAGGAAAATGCAACCTTAGCCGATATCTTTTTAATTGTTCTAGATGCTAGAATTCCAATTAGCAGTTATAATGAAGATTTTGATAATATCGCGCCTCAAAAACCAAGACTTTTTGTAATCACAAAAAGTGATTTAATGGATAAAAGTAAAAAAGAAAAGATCCAAAAAAGATTTCAAAATGAAAAACTTTTGTGACTTGATTTAAGACAACTAAAAAGTAGAAATCAAATCTTAAATCAATTAAAAGTTTTATCTCAATCTAAGATTAAAAAAGATAAAGAAAAAGGTTTTTTAACTAGCAACATAAAAGTTTTTGTATTAGGAGTGCCAAATGCTGGGAAAAGTACATTGATTAATTTAATGAGTCAAAGGAAAAGTTTAAAAGTAGCAAATTATCCTGGAGTTACCAGAGTTAAAAAATGAGTTATATCAGATAATTTTTACTTTATGGATACACCTGGAATTTTATTGCCTAAACATGAAGATCAAAGTGCAGCAATTAAATTAGCAATGATAGGGTCAATTGAAACAAATATTTTTCCTCAAAGATTTTTAGCAGAAAACTTTTTAAAAGTTATTGTTAAATATTATCCAAACAAAATTAAAGAAGAATTCAATATTGATATTGAATCAAAGGATGATTTGAGCGAAACTGAAGCTTATAACATAGTTGCTCAAATTGCTGATAATAAAAACTTTAAAGTTGATAAAAAAATAGATTTTGACCGAACATATAAATATATATTGAATTGAATCAAAAACTTAAAGAATGTTACTTTTGATTAGGAGAAAGTTATGGATCAAGAAATAAAAAACGAAAAGAAAAAATATTCTTTTGAAGAAAAAGTAGAAGCTTATAAAAAAGTGTACAAAAGCAATTTAGATCATTTGAATCTTAGAAATCAAATGAATATTAAAGCTTTTGGACTCCTATTTATTTTTATGATAATTCTATTGATCATAACTGTAATCGCTTATGCTTGACAAAATAAAGCGGCACCATCAATCACATATACAACTTTACTTTGAATTTTAATTTGTGTCTTTTCAATACTCACAATTCTTTCTCTTTATTTATTAATTTTATTTTTCATTGAATATAGTTTAATCAAAAAAATAGGTCTTAAAAAATCAGAACAAGAAATAGAAGCATCTATAAGAAAATTTGTTAAATTTGGATTTAAAAAATATCCTAAAAAACAAATGGAAATGCTTGAAAAATTCTAAAAAGCCTGTTTTATAGTGCTTTGGTAAAAATTGGCAAAAAAGAAAAATTCCACAAAACAATCATATGATTGTTTTTTTAACTATCTTTAAAGTATAATTTAAATATAAAAAACAAAGGGGGATTTATGCCAAAACTAACTGAAACTCAAGAAACAAGAATGAGCAACTCAATTAAAGAATTCGTTGAAAATCTTGAAAAACATGAAAACAGAATTTTAAATCACCCTGCAATTGAACCTAGAGAATATGATGGACTAGAAAAGCTTATAGAAATAAAAGGCCTATGTAAAGAATATAAAATTAAAGGCAAAAAATTTAGAGCCGTTGATGATATGAACTTAACATTGTACAAAAGTTAAAACATTGCATTATTAGGATCAAATGGAGCTGGTAAAACCACAACAGTTGAAATGCTTGTTGGTATTCTTAAACCAAGTGCAGGAAATATTAAATACTTTTTTGGCGACTCAATGGATAAAGGAAAAATTGATGCAACAAAAATTGGAATTCAATTTCAAGATTCCTCATATCCACAAGGGTTAACTGTTCGTGATGTAATTAAATCAATGAACAAAATCTATGGTAACAAAATGAAAGGTTTAGAGCTAGATTATTTAATTAAAATTTTTGGTGTTGATGAATATATAGATAATAAAGCAGCTTCACTAAGTGGTGGTCAACATCAAAGATTAAATGCTCTTTTAGCTTTAATTCATAAACCTGAATTAGTTATCTTAGATGAATTAAGTACTGGATTGGATATTAAAATTAAGACCAGACTTGTTGAATTTATTAACAATTTTGTTAAGGATAATAAATCAACTTTATTGATTGTTTCACATGATATTAATGAAATTAATACACTAGCAGATCGTATTGTAATTATGAACAAAGGCAAAGTTATTTTCGATAAAACCAAAGACGAAGCAGTAAAAGAATTCAAGACTCTGGCAGCTTGCTTAGAGTACTACATTTAAGGAGAATCATGGAAGATAAAGAAAAATTAAATGAAACAAACAATAATCGAAAAAACATCAAAAAACAAAAAAGAATTATGCAAAATTCTAAACAAATGAATTCTTTTTTAGGTGTTTTTAGTATTATTAATACACACTTTTGAAAAGCATTTGTTGGTCCTTTCTTTGCTTTTGGTTATCCTATAGTTTTTGTAATTATCTTAGGTGCTGTCTTTAATTATGAAATGATTTTAGGTTCTTCTATTACAATAGGACCTGTGGCTGTAGCTTGTGTTTCACTTCCTACTGCAATCTTCGAATTTAAAAAATCAACATTGCTTAAGAGAATTGGGGCTTCTAATATAAAACCGCTAGCTTTCTTAAGTTATACGGCCTTTTATTACTTCTTTGTTATGGTTCTTTCTGCTATTTGAACAGCCTTGGTTGCTTTAATGGTTTTTGGACCTCAATATTTTAATGAAGGTAAAGTATTATTTGAAACAACATTTGGTTCACAAACTATAGCTGTTAGAACAACTCCAATGAAAGACTTATTTACAAGAATTCATTGAGCTGGTTATATTTATAGTGCTATTGTTTTAACACTTGTAAGTATTGCTGTTGGTTTATTTATAGTTTCAGTAAGTAAAAGTATTTTAACTATCCAAGCAATAGGTTCATCATTATTAATTATTTCAATGTTTTTAACTGGACAAGTGTTGCCTATATCTCAAATTATTGGCCAAAAAGTAATGTGATATTTATCATACATAACTCCATTTAAATCGCCAATAACTCAAAACACAATGGCTTTTAGTGGTAGTGCATATTTAAGCTCATTATATGATGGTTATAACTTTACTGCTGGAGCTGCTGAAAAAATTTATGCTTTAAACAATTTGCAAACATTTTTGGAAACAATAGCTAAAAATACGGAAACAAATATAATAACAGCAACACAAATTCATTCTAAAGGCAGTGCACTTTTTCCTGAATCTGCTCAAATAAAAGCATATATTGATGAAATAGCAAAATCAATAAAAATGATAATGTTTAATTTGACGGATCATGCTATAAATAAGGATGATGCAATTAATTTAACTATTCGTTATAATTCATATAATATCTTTAATGCAACAGAAGTTTACAAATCGATTAATGCTTTAAAAACTTATGAAGGAACTAACTTAACATTTGATGAAGCTGTTAAAAACTCAATTTATGAAATTTTGAAAAAAGCAGTAAACGAAAATGAAAATGATAATTATATTAAGCTTTTAAATGGTATGTCAAATCAAGATTTATGTCCAAATATTCAAGATTATCCATATAAAGTAATAAATGGTAAATCTATTGAAAAAGTAAAAGACCTTGATTTAAATAAATTCATTGATGATTATCGTAAAGAAATGAATGAAAGTATGAAACAATCAATGTTAGCTGTAAAAACAAATGAAATTTACAATCTAACAATGAATAATATTAAAACCAATTATAAAAATTTTATTGATACAAATAAAGCTTATGTTCAATTATCTCAAGGTGGTATTAATAAAGATTCGATTTTAGAAATTGGCAGTGTTGTTGAAAACTATGTTAACTTTATTCTTCCATGACTTTGAATAGTTGTTTTAGTTGGCTTATCTCAAAAATCATTTACTTGAAATACAAGATAATAAATTATTACATGCTCAGTCATGTAATTTTTTATTATAAAAAATTGGCTTAAACCAATTATTTTATCTTTACTATTTCTTCATTTAATGATTTAAACATTTCATAGCATTTTTAAATATGCAGCAAAACCTTCTTTAATCTTTTTTTCAGCAATTTTGACTTTATAGACTAAATCCATATATTTATTAGCTGTTGTATGGTTTCTTTTTTCAAGATCTTCTTTATCATTAGTTAAATCTATTTGAATTTCAAGTTTATATTTTTTAAACAAATTTTGAACTATTTCTTTTGTTAGTAGTTTTCAATTTTCATTAAATTCTTGTTTAATAAAAGGAAAAACCTTTTTGTTATTATACAAAGCTTGATTTTTTTAAAGCTGCTTATATTTATCAATTTCTTTAGCAGGCTTGTCATACATTCTGTCTTCTTTAATGCATGAAGCACTGGTTAAAAGTAAGGTTGCTAAAGTACTTAATGCACTATTCATTCTTTTCCTTATATATTAATATTATAAAAAGAAAACTTGAGCAATGCTAAATGTGAGAATAATTATCAATTTGTTGCATTAAAACTTTATAATTAAACTAATAAAAAAAGGAGGTTAGTTATGCCAACACCACATATTAGTGCGCAAAAAGGTGAAATAGCTAAAATTGTATTAATGCCAGGAGATCCCTTAAGAGCAGAATACATGGCTAAAAAATTTTTAAAGAATCCTAAATTAGTTTCAAGCGTTAGAAATGTTTTATTTTATACAGGTAAATATAATGGCAAAGATGTAACAATAGGGGCTAGTGGTATGGGTTCAGCTTCAATAGGAATTTATGCTTATGAGCTTTTTACTGTTTATGAAGTTGAAACAATAGTTAGAGTTGGTTCAACAGGTTCATATATCAAAGAATTAAATGTTAAGCAACCTGTGTTAGTTAAAAGAGCTTATGCTGATGGAACAGGCTTTGTTGAATTAATGGTTGGAAATAAAAAACAGGAAAATTATCCTGATTTGGAAACTTATGAAAAGCTTAAACAAACAACCAAAGAAATGAATGTTGAATTGAAAGAAGTTTCTTGCCATTCAACTGATGTTTTTTATGGATTAAGATCACTAGAGGATACTATAAAAATAACTAAATGTCAAACTGTTGATAATGAATGTTTTGCTCTTTTTGCAACAGCACAAAGATGCAAGAAAAAAGCAGCTGCATTATTAAGTGTTTCTGAAAATATTGTAACTGGAGCGTCATTAAGTTCAGACGAAAGACTCAAAGAATTTAGTACAATGTTTTTAGTAGCCTTAAATGCTTTAACTAAATAAGGAGTTAATTATGAATAAAAGACAAGAAACAACTTTTTTCAAAAATAAAGTTACTTTATTAGGTGATAAAATTGAAGTAGGTCAAAAAGCACCAAATTTTACAGCCTTAAATAATGAATTAAAATCTGTTCAATTAAAAGATTTTAAAGATAAAGTAATATTAATCTCATCTGTTCCTTCTTTAGACACTGGAGTTTGTCAAATGCAAACTGTAAGATTTAACAAAGAAGTTGAACAAATTAAAGGGCTAAAAGTAATTACAATTAGTTGTGATTTGCCTTTTGCTCAAAGTAGATTTTGTACTTCATTTAACACTAATAATGTTGTTACTTTATCAGATCATAAAAATTTAGATTTTGGTCTTAAATACGGATGTGTAATAGAAGAATTAAGGCTCTTAAATAGAGCTATATTTGTAATTGATAAAAATGGCATAATTCAATATGTAGAATATGTTAAAGAAAACACAGATCATCCTAATTATGATAAAGCTTTAAAATGTGTTAAAAATATAATTTAGATTTCCGAATAACACTGTTTTATAGTTTTATTAGTTAAAAATGAAATAAGCATTTGAAAGTTTATTTTAATAGTAATATTAGTTGCTTTTTTAAATATAACTTTAACCTTAAATTTCTAACATTTACATAACAAAATATTTGTTAATTTAAAAAAAATATTACCAATCAATAATTCGCAATAATTCACTCAATTAACATTATTGGTTAGCAAATAATTTTTGCAGTAGTAGAATTTGCTTATCCATTGGCTAAATTTTTAGGAATATAATTTGTGCAAAGATTAGAATTTCATTGATAAAAATAAAGTTATTGTTTATAATGTTAAAAACTAATGGAGGAAAATTATGAAAAAATTATATTTTGGACTTGTTGGCGCTAGTGCCTTATTGCCAGTAATGACAGTTGTTTCGTGTAGTAACGAAGACAAGAAACTTCATAATGAAGCACAAAAATTATATGAAGAATATAAAACTAAGAAAGAAAAATTATACAAAGCTAAACTTAATGCAAATGAAATTACTCAAAGCTTGTATGATGATGTTGTAAACTCATCAAGACACAAAGATGTTAAAGACTGAGCTAAAGAATTTTTATCAAAAGACTGAGATAAAAACAAGAATACAAAAAAAGTAATTATTTTTAATTTAAAAAGATTTTTAAAATGAACAACTGAAGAATTAAATAAATATCAATAAATAAAAATTGAATTTTAAAAACCGGTATACAATGATACCAGTTTTTTAAAATTTTTTGCCAAATATACTACGTTTAGAAACAGTGTATGAAGACACATAAGTTTTGTAATATGCTTCATTAACTATAATTCCTAAAAACATAAAATATGTTAAGAATGAAACAAATAATGCAATATACATAAATGAGCCTATAATACCATATTTGTCATAGTTAATTAATGATGTTAAAAATCCAAAAATAGTGATAAAGATCATGACTGGAAAGCTAGTAATTAAAACGCCAGGAAATACTGTAGTTCAACTTAATTTAAAGCTAGGACTCATGGTATAAAGTAAAGTTAAGCCAAAGTAAAGCATTATTAATAAATAGAAAGAAAATGTTATATAAAAATATATATTTTTGTGATATGTTGACATATTGTAAGTAAAAGTTTGATAGAAGAATAGATATAAAGCAGCTGCTAAAAATAAGTAAATACTGATACCTAAAACAACAAATAATCCTTTAATTCTATTAGTAAATCAGTTACCTAAATTTTCATGTCCATAAATATAATTTTGACTGTAAATAAATTTGCCAAAACCACTTGAAGCCATTCATAAAGAAGCCAAACAAAGAATAATTAAAGAAGTTAATTGAGCGCCTTTTTTTGTGGTGTCAGGGATCTTAATAATAGAAGTAACACCAGGAATAATTCGAGTTAAAACTTCATTAATAAATAAAGTATGATAACCAGGAATTAACGAAAGTAAAACAATCATAATTGTAATAATAGGAACAAAGGAAACTAAGAAATAAAAAGCAGTTGAAAGTGGAATAAAAACAAATGATTTATCAGTAAATTTTTTGTAAGTTCTATTAATTAATTCGTTTGTTTTGCTTTTGTTTTTTCAACTTAATCTAGGGGTGGCTATTCACAAAATAATCATGATAAAAAACTTAACTATTTTTTCAAAAAATAGTCATTTTTTATTTTCTACAGGCACAATATTACGAGCAAATCAAGATTTTTTTAGACTGTTTCGGTATTCTTTTTTAAGTCTATTTTGATCTAATCCTGTATAAATTTTATTTTGATTATTATTATTTTGCATATTTATAAGATTATATATTAATTAAATTAAGCATAAAGATTAATTTAAATTATTCTAAACCACTTTTGTTTTTGAAAACTAAATTAATTGGACAACCAGTGTAGTCTAAAACTTGTCTAATTTGCTTTTCTAAAAATCTTTCATAGCTAAAGTGGACATATTTTTTATTGTTAACAAAGAAAACAAAAGTAGGAACTGGATCTTGAGTTTTACGAGCAAAATAAATATTTAAACGGCCACCATTATAAGGAGGAGCTGGCTGAATCATTTGTGTTTCTCTGATTAAGTTAGAAAGAACTGAAGGTTTAACATCACGTTCAAGATTTTGTTTAACTTTAATTATTGTGTCAATTAATTTGTCCATTCTTGAGTTTGTTTTAACTGAAACAAAACAAATAGGAACCCAAGGTACAAAATGAAATTTGTTACGCATTTTCTTTTCGAATTCAACCATTGTTTTAGTTTCTTTATTAATTAAGTCTCATTTATTAACCACAATAATTATTGGTTTATTGTTGTCTAAAGCATAGCCTATAATTCTAGAATCGAAGTGTGATAATTCTTGAGTTGAGTCAATTAAAACTAAAGATAAGTTAGATTCGTCAAGTGAACTCATAGCGCGTTTTAAAGCATAATGGTCAACGCTATCTTCAATTTTACTTTTTCTAGTAATACCAGCTGTATCAATGATTTCAAAAACTTCATCTTTGATTTTTACAATACTTTTAACACTATCTCTAGTAGTTCCCGCAATATCAGAAACTATTGAACGGTTTTCATTTGCTAATCTATTTAACATTGAACTTTTACCTGAATTAGGACGTCCAATAATTGAAAGTTTGAATAGTTTGTCACTCTTTTCATCATCAAGTCTAAGATGTTTAAGACATTGGTCTAAGACATCACCAACACCATTACCATGTTGAGCTGAAATAGGAAAAATATGATCCACGCCAAGTGAGTATCATGAATAATTAAACTGTGAAATATTATCTAATTTGTTTGCAACTACGACAATAGGTTTGCCAGATTTTTGAAGCATACTGTAAATCATTAAATCGTCATTAGTGATGTCAGAAGCACCATCAACCATAAAAATAATTACATTAGCTTCTTCAATAGCAATTTTTGCTTGAATTTGAATATGTTCTTGAAAAGGTCTATTTTCGATTTCGATACCACCAGTATCGATAACTTTAATTTCTTTTCCATTTCAAGAAAAGGTTTCATAAAGACGGTCCCTTGTTACTCCAGGACGGTCATAAATTATTGAACTTCTTTTACCAATTAGCCTATTAAATAGGGTACTTTTACCAACATTTGGTTTTCCAATAATGGCTATAACGTTGTTTCTCATTGATTATTTATTAAGTTTTTCTATTGTTTTTTTAACTAGTTCAATAATTTCAACAACAACTGCTTCAAAACCCATATTTGTACAGTCTATTTCTTTTGCTTCTTTAACTTTATGAAGAGGGTCTACTTCACGATTCATGTCTTGATAGTCTCTTAATTTAACTTCTTCAAGAACTTTGTTGTAGTCCATATTAAATCCTAAAGCAATGTTTTGGTTTAATCTTCTCATTGCTCTTTCTTCTGGTGTTGCTGTTAAAAAGATTTTTAATTCTGCATAAGGCATAATTTTGAATGTAGTATCTCTTCCATCCATAATGTAACCTTTACCTTTTTTAGTAATATTTTGAATTAAATTAACAACATATTGACGTACTTTTTTATATGTTGCAATAGTTGAAGATCCATAAGAAATTGAATCTTCTCTTATTTCAACAGAAACATTTTTTTCTCTTAAAAAGATGTTTTCTCTTTCATCTAAAGTGATGATATCTTTTTCTAAAGTTTTATTGATTTCTTCTTCATTATTTCAATCAACACCTAATAATTTTGCATTTAAAGCTAATGCTCTATACACACTTCCACTATTAATAAATGTGTATCCAAGCCTTCTTGCTACTTCTTGTGAAACAGTTGATTTACCTGCACCGCAAGGTCCATCAATTGCAATATTAATCTTTGTAATTTTTGTCATAGTGCTCCTTTAACTAATAAAATATATTTATATTTTAATATATAAATAAGAAATAATAAAAAAATCACGTAAAGTGATTTAAAAATTATTATTTGTATTCATTAATTAATAGCACTGTATCATTTATAATTTGCTTCATATTGTCAATAGGTTCAAGAAAATTTCTTTTAAAATTTTTATATTTTTCTTCAGCTTTTTCAGTTCCTTTTTTGTCTTTTTCATAGGGCAATTGGGAATTTTTGATTAATTTTTCTAATCTATCTACAAAGGTATATAAATCAACACTTTGAAGTTTATAATTTGAGTTAAAAACAATCATAATATTTTTTGCCTTATATTCTCATGAATTATCAGTTATAAATTTTTTAAAATCATTAATTCATTCATTTAATTTATTAATTCTGTTGTTTTTATCTTTAGGAGAAATAACTGTTGATTTTCATTCTCTTAATAAATTTATTGCAGCATGAGTATGTTTTCCATCACTCTCTGAAGAAGGATTTTTAATTGTGCTATAGTCAATTGACGCTTTGTCTTCATCATAATAACGATTTTTATATTCACTTTCAATTTCTTCATTGTATTGGTTTATTCATTTTAAAAAGCGATCTTGATATCATTTTTGTCTAGATGTTTGATTTTCAAAAAAAGGTCAACCGTTTCAGTGCGAATTTTCAAATAAATTTTTAAATTGAGATAATGTAGAATCATTTTTTATGTATTTAGATCAGTCATTTAAAATTGAATTTATTTCATCATTTATTTTCTTCAAATAGGTTACATTTTTATAAAAATTAAAATTAGTATTATCACTAGGAGTAGATGTAAAGTCTTCAAAATATTTTTTGAATTTAGTACTTTCATCAACATTATTAACCATAAATTTATATGCTTTTTCTTCAAAAGCTTTTGAGTTTTTTAATAAAAAGTTTTCAAATGTGAAATCAGTTATTTCTTTAGTTTTTTCATATTCCTTATAATAAAATGATTGATATGACAATTTGTGGTTATCCTTGTCCATAAATTTAACTAATTTATTAAAGTTATTTGTATAAATAGAGACATTTTGTTTTAGATTGCTAACAAAATTATTTTTTTCATATTCACCCATGTCTCAAAAACTACTTTGACATGCAGCTGATGTCAAAGGAGTGGTTGTGGTTAAAACTGTAAAAGAAATTAAAAATTTTAATTTTCTTCTTTTTGACATAATTTCTTTTCTTGCAATGAAATTAAAACTTTAAGATCTTTTATTTCTTTAAGAGTTAAAGTTCGATATGAGCCTTCAGGCATTTTTTCAACTGTTAAGTTAGCAAATGACACTCTTTTAAGATTAATAACTTCATGATTTAATGCTTTGAATAATTCTTTGATGTGATGGTATGAGCCGACATGAAGAGTTACTAAATAACTTTTAGTATCAACTTGGTCAACTATTTGTTTTGAAAACTTACCATTAACAATCAAACCAGCGTTAATTAATTTGAATTCTTTTAAGCTTAAAGGAGAATCAATTCTAACACGATATGTACGTAAAATTTCGTATTTAGGATGTGTTAGTTTATTAATCATTTCTCAATCATTTGTTAATAACAAAGCGCCAGTTGTATCATAGTCTAAACGACCTACAGGAGCAATTCTTTCTGAGTAATTAATTAAATCAACAACTGTTTTTCTGCCCTTAGGATCTTTAACTGTTGAAATTGTCTTTTTAGGCTTATTTAATAGGAAATATACAAAATTTGGTTTTTGTAATGAGATTAGTTGATTGTTAATTTTTATCTCATCGTTTTCACTTGCTTTGTCACCTAATTGAACAATTTTTCCATTAACTTTAACTAGTTTTTTTTCAATATATTCTTCAGCTTTTCTTCTTGAACAATATCCTGTTTGTGCGATTAGTTTTTGAACCCTAATCAATTCTTTCATTGCAAACCTTTCTACCAAAAAATTTATTTCATTCATTTGTCATAAATTTTTTGTTGTTCTTGTGTAAATATTATACCTTTTTCTTGGTTATATTCATAATTGCGTTTTACAACTTCTTTAAATCCTTCATCGAAGTTACTTAATGCTAGTTGTCTAAGCTTTTGAACTCCGCTAAAAGCTCGACCATCACAGATTTTATCTGAGATATATAAAATTTTATCAAGAGGTCCTAATTCTAGATCTAAAGTTGTATGAACTTTTATTGCTTTGACTATATCTTCATTATTTATTTGATAGATTTCTTTAACTCATAAAGCTCCACAAACTTGATGTAAAACATGATTTGGATAAATAGATTTGTTGTTAGCTTTTTTATCATATTGAGCAATAAATTTTCTACTTTCATTTTCACTTAATTCTTTACAAATATCATGAAATAGTCCAGCATAGTAAGCCACTTTTGCATCATATTTTATTGTTTTAGCTAACTCAGCAGCAAAAGTTGCTGCAAAAACTGAATGTTTTGCTCTTTTTGCACTAAGCAAAGCGTGAACTATTTCAAAAGCATATAAAAAGTGACTTCCTATATATTGGTTAACTTTTTCAGGTACCATTCAAAGTTGACCTTTTCTAACTTCAGTCGAACTTTCTTCAAAAAGATCATTGTTTAAAATTTTAACATTGAATTTTTTAGCATTAATTTTATTAAAATTTTTACTTCTTTTAAATACAACGAATTGAGCTGTTTTTGTTATTTCTTCGATATATTGTCATTTATTTAATTTTGGCAATAAATCGCTGCCTATTAAGAAATATAATTCATCATTTGGATATTGTTGTTTGAAATATCTAATTGTTTCAAAAGTATAACTAACGCCACCTTTTTTGATTTCAAAATCATGCACAGCATAATTGCCTTCTAGATTTTCAATAGCTATATTAATCATATTAACTCTGTCTTTATTAGGGGCTACTTTATTATTCTTTTTAAATGGGCTTATCGCTGTTGGTATAAAAAACATTTTGTCTAATTTAAGTGTTTTATAGGCATATTCAGCAATTTTGATATGTCCACTATGGATTGGATTGAATGATCCACCAAATAATCCTATTTTCATAATTCTCCTTAATTTTTATATTAAATTATAAATTCTTTTTTGTAATATCCTCTTGTAAATATCTCATTTGTATATTTTGTTTTTTCTTTTCTTTACTATACTCTGAAGCCAATTTTAATTTTTTGTCACGCAATTGATAATTGACATCGTTAATAATATTTTTAATTTTAATAGTTTGAGAGCTTTCATCTTCAGGAGTTGTAAAAATACTTAGTTGTTTGCCATAATCAAATTCATTTACTAATTTATGTAATCTAACGCCAATACCTCTAATAGGTTCTTCATTTCACAATATTTCAAAAAGTTTACTTGCTTCTTTAAAAATAATATCTTCATCATTAGAAACAACTGACATTGTTTTTTGTTTAGAATGTCAAGTTTTATTTGTGTCTCTTATTGAACATGAAATGACTTCTGGTAAGGTATTTCTATTTTTTGCTCTTTTTGAAACTTTAACACATAAACTTCTTAGAATGAGCAATATTTCCTTGCGATCATCAATATCATATGTTAAAAAAGTAAGTTCATTACCTATATTTTTTAAATCATTATGTTCATAATTTAATTGGTCATTGCCATTGCCTTGAGCTTCAAGAATTATGCCTTTAGTTTTAACACCAAAAATTTTTTGTATTTTAAAATTTTCTCAATTACATTTTGCTAATTTGCCAATAGTATCAATATGCATTCTAATTAATCTCTCAGCTGTTGGAGCACCAATACCGAAAAATTCAATAATTGGTAAAGGCCAAATCCTTTTTTCTATGTCTTCCTCTTTTGTTTCTAGAATTCCAAATGGTTTACCTAAATTAGTTGATAATTTTGCTAAAAATTTGTTGTATGAAATTCCAATTGTTGCAGGAATGTCAAAAGTATTTAATATGTCACTTTGGATACTTTTAGCCAAATCTTTTGGATCTCTATTTTTGATAATATCAGTTACATCAAGCCAACATTCATCAATTGAAAACACTTCGATATTTTTTGTATATTTTTCACTAATAAATTCAAAAATTTTATTTGATAAAGATAAATATAAATCAAAATTAGGTTCAATAAAAACTGTGAGAGGATCTATTTCTAAAATTTTATTTTTAGGTCAGCCAACTTTAGCGCCTTTTCTTTTTAACTCATAACTAATTGAAGAAACTATTGATCTTTTTAATCTTCTACCAATAGCTACTGATTTTCCTTTTAATTCAGGATTAATTGAGCGATGAGCACTTACAAAATATGAATCAAAATCCATGTGAAAAATTGTTTTTTTATTTTCCATCTTGCAATTTTTCCTTTATTGCTCTAGCTAACATTAAAGCACAATTAACGCGATTAAATTGTGTTTTAACATTATCAAAAATAGCTAAAGTTTCTAGCTTTTCAATATCATTTTCATTTAATTTTTCATCTAGTATGAATTTTTCGTAAAGTTCTATCAAACAGATGCTTTCGTCAATTTTTTTACCTTTTAATAAGTCGATTAATAAATCAGTTGAAGCTATAAAAAATGCACAACCTTGACCATCAAATTTAATGTCTTCAATTTGATTATTTTTTATATTTAGTTCACATTCTAAATAATCAGCACAGCTTTCTCCGTGCTTAATAATTTTGTTTTTTAAACTTGAACTTTTGTGTCTAGGTTTCAAATAATGTTCCATTATTATTTGACGTTTTTGATCTTTATTATAAGACAAGGAAATCACCTCCTTCTTTTAATGCTTTAACTAATTTATCAATATCTTTTTTGGTGTTATAAATACCCAAAGAAACTCTTACAAAAGACTTTTCTTTGGTGATTTTTTTAAGATAATGATCACAAAATACACCTGCTCTTACATAAATATTTTTTGTTCCTAAATAATGTGAAACATCATGACTATTATAGTTTTTTATATTGAATATTATAATGTGATTTTTAGGATTGCTATATAGTTTTATGCCTTCAATTTTGCTTAATTCTTGATAAGCATAAAAGCTTAATTCGTCTAAAATTTTTTGAGTTTCACAATAACCAATTTGATTAAAAAAGTTTATTGAAACACTAAACATATTAATTGCTGCTAAATTAGGTGTTCCAGCTTCAAAGCGATCAGCTGCTGTTGACAAAGTATATTGTTTTTCACTGTCAATATTGTTAATTATTCCACCGCCAACAAAAGGTGCTTCAAGTTTTTGTAGAATTTCTTTTTTAACTGCTAAAACTCCAAAACCGGTAGGTCCATAAAATTTATTTGAGCTAAAAGCAATTGCATCACAATTATTTAATGAAACACGTTCATAAACTATTGCTTGAGCCGCATCATTAACTACTAAAGTATTAAATTTTTTAGCCTTTTTGTAAATAGCATTTAAATCAATATTGTTTTCAAAACTATTTGAAACTTGACTTACTGCAACAATTTTAACTTTTGAATTTAAGTCATCTAAAACATTTTGACTTATTTTAACACTAGCCCCAATTTCATTAGCTATTTTAATTCAAGGCGCCATATTTGAAGAATGATTATAAACATCTAAAAGGATAATATCCCCTTTTTTAATTTGATTTTTGATCATTAAAGCAAACTTGTTTAGTGAATCAGTTGCTCCTGAAGTAAATATAACTTCTTGTTCTTGACAAGACAACAAAGTAGCAACTTTTTTACGTAAGTTGTTTATTTTTTGCGCGCTTTCAATTCCTAATTTAGTGTTAATAGTACGGGTACTAATTGACTCATTTAAATAAAAATTGTTAACTGCATCAATAGCCATTTTTGGTTTTAAAACTAATGCACTACTATCCAAATAAGTTATCTTTTTTGCTAAAGGGAATTGTTCTTGAATTAATTTGTCTAATGTTTTCATATTTTAAATTATAAAGTTATATTTAATATTTGTATACAAATGTTACATATAAAAAATAACGTTTTTATAAAAAATATATAATTAGTACAACAACTTAAAAAAGGGAAAAATGAAAGAAGTTAAGAATTAAAATAGAGCAATATCTTACTAATGAAAAAGAATTGCTCTTATTTTTTATAATGTTCAATAATTTTTTTGTATTTTTCTATAACTATTTCAATTGCTTCTTCTCATGAATTAAATAAATTGTTCATATTATTTTTCACATTAATTAGATTATTTTTATCTTTAAAAATTTGTTTAATTTTTTCGTACATATTGTCAACATCGTCTTGGCAAATAAAACTATTTTGTCCATCTATCACATTTTCGGCACATGCTGTGTTTTCAACAACTAAGAGAGGCAACTTTAAAGCTGCAGATTCTCTAACAACAAGTCCATAAGTTTCATATAAACTTGGATAGAAAAAAAGATCATGAGATAAATAAATGCCTTTTAATTCTTCTTGATTTTCTATAGGGCCAGTAAACAATATTTTATCTTTTAAACCTATTTTATAGGCATATTTAACAATTTCCTTGTAATTGATATCACCACCAACCATTGTTAAATGGTAATTAGAATTTTCTTCAACAAGTTTTTTATAAGTTAATAGAACTTTTTTAATATTTTTTTCCCAAACCAATCTTGAAACATATAATAAATTTTTATTATTAGTGTTTATATTTCATTTTTTTATAACTTTATTTTTTAATTCATCTGCATTTTCTGGATAAAAGAAATTTGTCCCTAATTGTACAATTTCACTTTCTTTTTTATATCCATATTCAATTAAAGCTTTTTGCGCAACTTTTGACACAGGTCAAATGTAATCAAACTTATTAAGTTTTTTAATCATTAAATGTGTTAAAAATTTTGCAATTGGCTTAGACTTTGTAAAACGCAAAAATGAATCATAAAACAAAGTATGAAAGCTACAAATAGAGGGAATTTTAAATTTTTTTGCAATTTTATTTGCGATATCTCCGGCATAAAAAGGTGTATGAGAATGGACAATATTAGGTTTGAAATTTTCAATTTCTTTTCAAATTTTTCTACTCAAAATAGTGTTCATTACTTCATAACCACCAATTTTAGCTAATTGTCTAGGTATTCTAATGACAGAATAATTAAGACCTTTATCTTCATCTAATTTCATTTTTTTGTATTCTGGAGCCAAAACCAGAACTTTAACATTTTTTTTAATAAAATTAGAAGCATAATTTTGCATTACCCTGACAACTCCATCTATTACAGGAGTAAAACTATCATTAATTAAAATAACTCTAATTTCACTATTTGTCATTTTTCTTTGCCCTTTCTTTTTTACAAAAAACTTTCATATCATACATTCCTAAATGTTCAGACATTTTGTATTTTTTTGGAATTCTTCAATTTGGACCATAGTTTTGTAAAAATATTGTTTCATAATTTTTTGGCACCCGAATTTTTATATCTTCAAATTTAACATCAATAGTATCAAAAAAATTGATATTTTCATATATGTTAATTTTTGTAGCCTTTTTAAAAGGCCAATTAATTACATGATGAATATTTGAATTTGGTTCATACAAAGTCTCAAGAGCTTTTTGATAATTTGGTTTTTTAAACATTCTTGTTCAAAATAATAAAACTTTAGCAATTTTCATTCCTCATTGAATATCAAAAAACTTTCTTTGAGAATAAGCGTTCAAATAACCCAATTTATTTTTAAGAGATAAATATTTTTTTATTCTTTTTTTAGTTGTTGGTATATCGATAAAAAGATCTAAAAAAGTTACATCTTTTTGAGAATCTTTATCTTTTGAAAATTTAGGGAACATTAAAAAGCTGTTGCCTGTTTCTCAGTCTACAATTAAATTTCTGTAATTTTCCTTTAAAAATTTATATGTTTCAATTGAAATTAATAAATCAATATCATCATCTCAAGGTATAAATCCATTGTGTCTTATTGCTCCTAATGCTGTTCCATACATAGCACAATAATCTAAATTGTGTTTATTACAAATTTCAATAAACTCTTTCAACAGTTCATAAACTTTAGTTTGTTTTTCATTCATAGTTTTTATTATACTTTAAAAATTTTTTGGCAATAATAAAAACTATTATCTTGAAGATAATAGTCTTATTAATTATTTAAATTGCATTTTTGCATTGATCAGTTTCGATATATTCTTTTAGATTGTCATAAGTGTATTCAACCATGTTTTCAACAGCTTCATCAGTATAAGAACCAATGTGAGGTGTTAAAACAAAACGAGGATAGAATTCTAATAATTGGCGAAATGTTTTGTTTTCTATTTCTGCATGCTTTTTAAAGAAAATTGATTTTTCATTGTTCAAAACATCTGTAGCAATAGCTTGAATTTTGCCACTTTTTAGACCTTTTAATAATGCTTCTTCATCTTGAATTTGGCCTCTAGCTGTGTTAACAATAATTACACCTTTTTTCATTTGCTTAATTAATGCATCATTAATCATTTTGTCGTTTTTACCTTTAATATAGGGGCAATGGAAAGAAATGATATCTGATTTTTTAACTAATTCTTCTAGAGTTTTTGTATATTTAACAACTTTTTTGCCTTCTTCTGAAGGATATAAATCGAAGCCTAAAACATTGTTTGTCATAACTTTAAACATTTTAGCAGCTGCAAGTCCTATTTTTCCAACCCCAACTATTCCAACTGTTTTATTTTTTAATTCAGTAGCAAAACCTTCAGGATCAATAGTAAAGTCTTTGTTAAAACCTTTATGTCCAAAATGAAGTGTTTGTCTTGATAACATTAAAGCATGAGCAAAAGCTAATTCAGCAATTGCTGTTGGTGAATATGAAGGAACTCTAGCCATTTTAAAGCCTAATTTATGAGCATATTCTAAATCAATATGGTCTGTTCTGACAGTTCTTGTTAAAAGATATTTAATGCCATTTGCTTTTAATGCATCTAACATTTCTTTATTTATTGAACCATCAACTCTAACAATAACAGCGTCAAAACCTTTTGTTAAATTGACATTTTCCATAGTTAAACTTTCACTATGAATTTCAAGTTTATAACCAAATTTTTTGTTAATTTCTTCAAATATTGGTTTTTCAACATTTCTAACTTTAAAAGTAATAATTTTCATAATTTTTCCTACATAAATACTAATTCTGCAACTACAATTCAAATAGGTAAAAAGATAACTGCAAGTAATGTACTAAATGCTGAAGCAGAGCTTGCTAATCTAGCACATTTATTTTCACTAATTGCAAAACCAACAGCAACTGTAGCAGGTGGTGTAGCTGCAAAAATAACCATTGCAATTCCTACTTCTTTTTTAACTAATCCACCTTTGTTTAAACCTCAGAAGATTAAGAAGATTGCAAGTGGAATAACAATCAATTTAATTAATGAATATATTCAAACTCATTTATCTCCTGCCGCTTCTTTTAATTTACTTTCAGCAAGTGTCATACCAATAGCTAATCAAATAAGTGGTGAACATAAACTACCTAAAATATCAATAAATTTATAAATATATGGTAATGTTGTTTTTCATGTAAATCAACCAACTGGTTTAGTGGTTTTAATATATTCATTATTTGCATTTAACATATAAAATACTTTTTTGTCTGGTGTTAAAACTGTTTTAAAGTTTTCACCAAAAGTAGCTGAATATTTAATAACTACATCACTTCCATTAACTTTAATAGTTTGACTAAAATCTTTATATGTTGCTCCTGCACCTGGTATTAATTGATTCAATCAAAGAATTAATCCAGCAAAAGTTGCAATAATAATTGGGTTTAAAAACATTGATTTTGCTGAAGCTTTAATATTTTCTTTGTTGAATTTTAAACCTTTCATTTGCATGAAGCAGAATGAATATAAGAAAATTCTATAAGGAACATTTCAAATATTTGCTGCTAGAACACCATTATTATTTGAATATAGTTCCTTTATAATAGGCATACCAAAGAATGTTGTTGATCCGAAGATTAATAACATTCAAACAACCATTGCGCGATGACGAGATTCATCAACTGTTTTTGTTTCACTCATTTCACCAATAATAGTGTTTTCTTGAGCATTTTCCACTGATTCTGGTGTTTTATGAGGCATATATTTAGTTCATAATCAAGCCACTAATGAAAGCAAGATATAGAATCCAAATGCAATTCCTAAGATAATACCTTGTTCTTGAAGAAGTCCAAGACTAATATCGCTCATAAAGCCTTTGAGTGCTAAAGCTGGTAAAGCAATTTTCATAACTACGCCAACAAGTGCAGCTTTTCAAGTTGATTTAAATCATTTAACTTTAACCAACAAATAACCTAGTCCGATTATTACAATAGAAGCAATAATTGCGCCTCAAAGATTTACATTAGTTAAAGTTGAAACTAATTTCTTTGTAACATCCATTTTTCTCCTTTAATAATTTGCTCATAAATATTATATTTTTTATATTATAAAAAATGAAATAAATAAAAAGGTTTTGTGGAAATTTTCTAGAAATTGGAATTTTTATAATTGTGAAAATTACTATAAAACAATGGTTTTGGCAAAAAAGCTAATAAAAATACTTTACACTTTACTAAATAAATTTAGCAGTATAAAGTATTTGAATTAATTATTTTGTTCTTGGTTTTGATGACTATTATTATCATTTGTTTCCTCTGCTTTTAATTCTTTCTTTTTCAATTTCCTAAAGATAAATTTATCATCAGGTATTAAAGTTATTATTTGGCTTATTAGAATTAAAGAAGCTAATAAATAGCAATATCAATCATAAAATTCAAGTCTTATCAAATCAAACAATATTGTTCAACCAACATAGGAAATATTTAATCCTGTAGCCATTGGAACACCTATTTTATCAAGTGCATAATAGAATGTCATATATGAAATGCAACCCACAATTCCAGAAGCAACAATTAATAAAATGTTTGTATTAGCAAATAGTTCATCCATTTTTTCATAGATTTTTGCTGATGGTAAAACAATGCTACCTAAAACAACCAATGAAACAACATATCTAAAGAATATAGAAACAAAAGGATCAATAGCATTTTTCATTGATAAGGTTGATAAAAATGCTTCCAAAGCTCAACAAAGTGCAGCTAATATTGCAAACAAGAAACCTCATCCATTTTTAATATCGCCTTTATGAAATTGTAAAATTCCTAAAGTTAAAATACATAAAAGCGAAATTATTGATCCAAATAAACCATTTTTTGTAGTTTTTTGTTTCAAAAAGATAAAAGCAAGCATTGCGCCAACAATAGGATAACAAACTGTAATTGCACTACTTATTCCAGTACCGACATAAACAATACCAAGGACATACATTGTCATACCCATTGGTGCCCCAAAAAGAGAGCTGAGTAATAATATTCATATTTTTTTATTTTTGAAAGCTGCAACAAATTCTTTATGTTTTTTAAATGAAAAAATAATTATTATGCTAACTAAAAAGCCAAATGTATCATTTAAAAAAGTTATAGCTAAAGCTAATTTAGCTGGGCCTCATTCTCCTTGATTAAAAAATGAACCGGAAGGAATTAAATTTGTAAAAACAGAAATTAAAATTCCATTTAATGCTCACATAATCCCTGAAAATAAAGCAACAAATTTATTAAATAAATTTTTATTTTTAACTTTTTTATCGTTTTGAATTTCATTAACTTCTACATTTGAATTTTTATCATTCATATTTCACCTTGTTTACAAATTTTAATGTTGCAGAATAGTAAATTTTGTAATAATCTCTGAAGAATTCATTAAATATTTCTTTATATTCGCACCAATATGTTCATAAGAAACCTATTGAAGCAATTAAAATGTATAATCTATTTTTTTCTTCTTTAGTTGGTTTTCTTTGTAAATATGTTTCTAAAAGTCAATTAGCTTCTTTGACACCATATTCATTATCCATGCACATACCAGCTAAATCTAAAAATGGATCACTATTAGCCGCATATTCTCAATCAATTAATTTAATTTTATTATCCTTATTTATTAAGATATTTCCTGAAGTTGAATCAATATGACATAAAACTTTTGGATAATTTTTACTTTTATGATAAGCAAGCATTTTTCTAATTTTAGCTTCATTGTCTAATAAATGTTTTGGAAATCTTTTGTATTTTTCTCCAATTATTGAAATGTAATAGTCCAAAATTTTTAGAATATCAAATTCAAAATCAACTGATAAATTTTGATTATGTAGTTTGCGATAACTATTTAAAGCTAATTCCATTTCTTTATAATTGTGTCCATCAATTGTTTTTGAGTTATTAATTCATTCAGTTATTTTAAAACCATGTAAAGGATCATCAATATTATTTTTGGTTTCAAAATAAATTAAATTTTCAACTAATTCATTGCCTTTTAGTTTTTGATAAATTTTTTCTTCTTGGTATCTATTAATTAAACTCTTTGAGCCATCTTTTGGTATTCTAGCAATATATTTTTTATCTTTTATTTTAAAAGAAAATGAGTTATTAGTCATTCCTTTTTTAACTGGTTGAATATCTAAAATATCTTCAAATTTGGCATTAAAAACTTCTTTAATTTTGTTTAAATAAACATCGTTTATAAAGCAAGGAGCTTTTCTATTAAATGTCAATAAATCTTTGAAATCGTCTATTTCATAGACCTTTTGGTCACAATTGTATTTCTTAAGCTCTATATCTTTTAAGATATCCATCAAAGCAAATTCTCAATAATCATTTTGCTTTGAAATTGGATTTTCAACTCTCTTAATAATTGCATTTTTAAATTTCAAGAAATCTTTTTCATGAATAAATGAAGCACCTGTAATAAATTCAAAATTATCACTTTCATAGATTTTATTTAAGTCTCTAATTCTAAAGTCAACTATGTTTTTATTCTTTTCAATAATTTCTCATTCAGCTTTATTTGTTGTACTACTCAAAGTATTAACCCATGAGTAATTAAATTCCTTATTAAAGATATCATCTAAAAATAAAGTGTCATTGGTTGTAATTAATAAACCACCCTTAATTGTTTTCAAATCTTTAATTGCTAGATATAAACTAAATAATGAATTAGTATCTGTGAATTCTTTGTTGAATATCTTTTTGATATTTTCTTTTTTAATTAAGTCATTTCAAGATTGATAATTGTAGCCAGTTACTATTGTCTTATTTTTATAATCTTTTAAATAAGATAAAAGATGAGGAACAATTTTTAAATCTTTTTTAATTTCTAATAATCCTTTTGGCGTCTTAAGTGAAAGAGGCGTTAGTCTTGAACCAAAGCCAGCAGCTAAAATTATTATTTGAGTATTTTTGTCCATTTTTCTCCTAATTTTGTTATAAATTATATAATCAATGATATTATAACCATATATAAAAACTATAAATTAAGCAAATGTTCAAAGATGCTGAAAAAAATTTTGTATTTTATTAAATATTTGTTTCTCAAAAAATAAATTTAATATATAATAAAAAAGCAATTTATAAATTGAATTAACGTTTAAGGAGGAAGTCATGAGACAAACCACTATTGTAAACAAAGAAAAAGCTGACAAGAAGTGATATGTTGTTGATGCTGAAGGACAAGTTTTAGGTCGTTTAGCCGCTTTTGTAGCTTCTGTTTTAAGAGGTAAAACAAAACCTACATACACACCAAATGCAGATATGGGTGATTATGTAATTGTTGTTAATGCCGAAAAAGTTATTTTAACAGCTAAAAAAGAAGAAGATAAAGTTTACTACCACCACTCAGGATATCCTGGTGGACTAAAAAGTATTACAGCTGCGAAATTAAGAGCTAAAAAACCTACAGCTCTTGTTGAAAAAGCAATCTATGGTATGTTACCACACACAAAATTAGGAAACAAACAACGTAAAAACTTAAGTGTTGTTGCAGGTCCTGACCACAAACACCAAGCACAAAAACCAGAAAGTTTAGAGGTAAGATAATATGGCTACAGCTAAAAAAGTTACAAAAAAAGAAGTTGTTGAATACAGAGGACTTGGTCGTCGTAAATCATCAACAGCTCGTGTTATTTTGAGACCTGGAAAAGGTAAATTCACAATTAACAAACGTGAAGCTCTTGCATACTTAACATCAAATTTATATATTCAAGATGCTGAACAACCTTTAGTTTTAACAGAAACAAAAGGACAATTCGATATAACTGTTAATGTTTCAGGTGGTGGTTTAAGTGGACAAGCTGGTGCTATCCGTTTAGGTATTGCTCGTGCTTTATTAGCAGCTTCAGATACATATAGAGAAAAATTGAAACCTGCAGGAATGTTAACTCGTGATGCGCGTGTTACAGAACGTAAAAAACCAGGTTTGAACAAAGCACGTCGTGCAAGACAATTCTCAAAACGTTAATTTCACTTTTTTATAATAATATTAAGTTTTGCTCAAATTTTGAGTGAAACTAAAATGCGAGCGTAGCTCAGCTGGTTAGAGCACACGACTGATAATCGTGAGGTCGATGGTTCAAGTCCATTCGTTCGCACCATTTCATTAAATTGACCAAAACCCTTGTTTTATAGGGTTTTTTATTTTTTATTTTTTCTCAAAATTTTAATTGCTATATAATATAAAATTAATATGATGATTGATTTATGATGTCGAAATTTTGAGAATCAATTGTTTTAAAAAGGAAAAAAATGAAAAAAGATCAATTACCTTATTTGCCAAAAAATTTAAAAAGAAGACAACAAAAAAGTTTTGAATTATTAAAAGTTTTAGACAAAATTTGCCGTGAAAACAATTTGTGATACTCACTTGCTTATGGTACTCTTTTAGGAGCTACAAGAAATAAAAAACAAATTGCATGAGATACTGATATTGATGTTCTAATTACAGAAGAAACATATGAATTCTTAAAAAAACATTATCCACAATATATTTTGGATAATACAACATCGCATCACTTTCTTAATTTTCCACGTTTTGTTGATAAGATTGATCAAAATGATCCTAACAATATTTATATCGATATGTTCATTATTGTTCCTTCAACATGGAAGAAAGCTAAAAAAATTAATAACTTTTGATACAAATTAAGATCAATGAGAAGAGCTTTAGCTCCAAGAAAATGATTTATTAATATTTTTGGAAATATTTTAATGTTTATTTTTGGCTGAATGTTTTTATTTTTAGTACCACCTTTAGATATTAAAACAACTATTGAAAAAACTAAGGTTTCAGAAAAAGAAAAAGAAATGTATTACATTACTAATTGGCCTAATAAAAGAATTTTTCATCAAGTTACTTTAACACTAGAACAAATTGAAAATGGCATGCAAGATATAGAAATGAATGGACATTATTTCAAAAGTTTAAAAGAAAATGAATCAGTTCTTGAAAGATGATATGGAAAGACATGAAGAATACCAATTCAAACTAAACATCCTATTTATTATGGTTATTATGAATTTAAAAAAGTTCCAAAAAAATATCGAGATTATTACAAAAATGAAAATGCAGGCCAATAACCTGCTTTTTTAATAAAAAAAACTCAAATTAATGAGTCCTTTTTTGGTCTAAAGTGATATGGTGCCCAAGACAGGACTTGAACCTGCACGGATCTCTCCAGTGGATTTTGAGTCCACAGCGTCTACCATTCCACCACTTGGGCAATTATAAATTATTATAAATGAAGTTTTATTTTTAGTAAATAATTTTTCTAATAAAGCAAAAAAAGTATAAAAAAATAGCAGCTCCCTATTTTCCCATCTCTGGTATCGTCGGCACTAAAGGGCTTAACTACTGAGTTCGGAATGGAATCAGGTGGGTCCCCTTCGCTATAACCACTGGATATATTATAACATGCAATTTAAAAAATCAAGCAAAAAAATAAAAAATTTTTTTCAATTTTTTGAAGTAATTTTTTTAGTCAAAAAACTGAGTAAAAAATATCTTTGTTTAATATTAATTTTTATATAATTGTAATGTATTTATTAAGGAAGAGATGAGAAAAAATAATAAACAAAATAATAAAGAAAAAGAAAGCAAATTTAAAAAATTTTGAGAAGAATGAACAATCTTTCCAAAGTGAACTATTAGAAAAATTTCTTTTGTTGGAATCTTAATTGCAATTTCGGTTGTATTTTTAGTTATTGCTTCTTCTCTTGTTCCTATTGCTTCAATTCCAACTTATAAAATAAGTTTTATAGGTTTGCCTATTAAAATTAGTGGTTTTATTTTTGGACCTTTAATTGGTGGAATTGTTGGACTAATTAGTGATCTTTTATCCTTCCTTTTTGTTCCTTCTTTTTATAATCCTATTTATACTGTAGCAGCAATGGTCGATGGAATAATTTCTGGTTTAGTCGGCTTATTATTTATTAAATTATTTGGTTACTATTTTGGTGGTGAATTTCAAGATAATGTTCATGAAAATAGAATTTGAAAATTAAGCAAAAAACTTGATAAACTAAAATTAAAAAATACTAATGATAAAAAAATACTTAAACTAGAAAACAAAATTATTCTAATTCAAGAAAAAAGAAAAAGTATTCGGATCAATGGAACACAAAATATTTTGCTAAATATTAATTTATTTGTTGCTGTTATTCTTTTAGCTTTAATATCATTATTTATTGTCTGATTAGTTGGCTTTGTAATTCCTGAAAGCATTATTAAAAAAGGTGTTATTTCGAATAGATGAGGGCTTTTAGCAACAATGGTATCTGGTTACACATTAATGTTCTTCTTTATTATAATAGCTCGCTTTAAAATGAAACCATCTAACTATTTAGTTATTGTTCCAATTGTTATTTTTTCTGCTTTAATTGAATTAATTAATGTGCCTCTTTTATCATTTGCTGACCAACAATCATTGCAAAAAGGAGAAGATAATAATATCTTTGTATTTGTTTTCAACCATATTTTATTTAGCCCTGTTAAGATTTGATTTAACATGTTTATTATTTATTTTACATACAATATTATTGCTCCTCTTATTTATAAAAATAAAAGTATATCTTATGATAGTTAATAAGTGAACCTCAAATTTCAAACTTTTTGGAAAGGGGTTCATTTTTACGCAATTAAAATTTAAAAAAATAAAAAGGAAGAAAATCTAAAATAAATTTTGATGCTCAAAAGGCAACAAATTTTAAAATTAATATTGAAAAAGTAAATTTTTTATGAGATATTAAAATCACAAAATAAACCTGACAAAGATGAAAATTTGAAAAAGATTATATTTGACTTATTTAACTATAATAAAGGACTATACGGTTATAGACGTATTACTTTTGCATTAAGAAATAAAGGAACAATGATTAATCATAAAAAGGTTTAAAAATTAATAAAATCATTAAATCTTTTTGGCAAAACATTAAGAAAAAAATAAGTATTCTTCATCCAAAAGGATGTTCACAAAAAACATTCCAAACTTGTTTTTAGATAAAGAAAATATCGCAAAATATTTCTTAAGATACAAAATTTTTTAGTAATAAATATTTGAAAATACTAGGAACAGATGTTATTGAATTCAAATTAAAAAAATGATAAAAAAGCATATTTTCTTCTGTGGTTGATTTTGAAAATAGTGAGATTTTAGGTTATTCGATTTCTAAATCACCTAACTTAAGAATGGGGGGTAAAATGTTGGAAAACGTAGAAGGGAATGAGCATAGCTTAAACAATGTATTATTACATTTTGATCAAGGATGACAATATACTTATGAAGATTATATAAATTATTTGAAAAAGAAACAAACAATTCAAAGCTTGTTAAGAAAAGGAATCGTTTAGACAATAGTCCCGCTGAATGTTTATTTAGTGTTGCAAAAAGAGAATTTTAATTTTGAGAAAAAATAGATTTAAAAGTTTTAAAGAACTTAAAACTACTTTAGATTATAATAATGACAGAATTGCTAATAAATTAAAAGGACTTAGCCATATTCAATACATGAATAAGTCCAAACATAATTAAATAGTTCAATAATTGAGGTTCAAACTATAATGATTGTCTGTGCAATTATTTTTTTATATGTTGATTTTTTATATGCTTTTTAGAAAAAGTCACAAAAAATATTAAATTCTAAAAACATTACCATTAAATATTATTTATATAAATAAATTAATATTAATTTATAATATTTTAAAATATAATTACTTTATAAGTTTTAAAGGAGTCTGGAGGTTGTATGAAAACAATGTTAAGTATTGCTCTTGAATATATTTCTGAAAAAAGTAAAGATGGTAAGAATATCAAATTCAATGATATTTTTAAAGAAATCAAAGCAAATTTAAATGACAAATGAGTAGCAGAAGCTGAAAATAAAAATTTAGATTTCAAAGATCTAGAAACAAATAAAAAAGGCGAATTATATCGTTTATTAACAGTTGATTCACGTTTCGTTCATAAAGGAAATAATGAATGAACAATTAGAAATGGTTTTGAAATTAATAAATAATATTTTGAAAATAGGAGTACAAAATGAAATTAGTAAATGCATCAAGTATGCTTAAAAAAGCTAAAAAAGGAAAGTACGCAGTTCCTCACATTAATATTAACAATTTAGAATGAGCTAAAGCAGTTTTATTAACAGCTCAAGAAGAAAATTCACCTGTTATTGTTGCAACAAGTGAAGGCGCAGTTAAATACATGGGAGGTATGAATATCATCGTTGGAATGATTAATGGTATGATGAAAGACTACAATATCACAGTTCCAGTTGCACTTCACTTAGACCATGGAACATATGAAGGAGCTAAAAAAGCTATTAAAGCTGGATACACTTCATTAATGTTTGATGGTTCACACTTACCATATGAAGAAAACTTAGCTAAAACAAAAGAATTAGTTAAATTAGCTAAAAAAGTTAACATGTCAATGGAAGCCGAAGTTGGAACAATCGGTGGTGAAGAAGATGGTATTATCGGTGCAGGCGAATTTGCTGATCCAGCTGAAGCTAAAGAAATGGCTGAAACAGGTATTGATGTTTTAGCAGCTGGTATTGGTAACATTCATGGTCCATATCCTATAAGCTGAAAATCATTAAACTTCGACAAATTAGTTGAAATTAGAGATGCAGCTAAAATTGGTATAGTTTTACATGGCGGTTCTGGTATTCCGGCTGATCAAATTGCTAAAGCAATTAGTTTAGGTGTTACAAAAATTAATGTTAACACCGAATTACAACAAGCAAATCACAAAGCTTTAAGAGCTTATATTGAAGCAGGAGAAGATCTTAAAGGCAAAAACTTTGACCCACGTAAACTTTATGCACCAGGTTATAAAGCTATGTGTGAAACAGTTAGAGCAAAAATTAGAGAATTTGGTTCAAATAACAAAGCTTAGTTAAAATTTAAGAGGAGTTAAAAGCCTCTTTTTTATTTTTCCATTTTTTTAAATTTATTTATAAAAATGACTAGGTATAAATATGAAGAGATATTTGAAACTTTTTTTAAATTTTTTGCCTTTTACTTGTTTATCTGTTGCTGGTTGTCGACAACAGATTATTAATCCACAATTAAATCAAGTTTTTGATGTCAAATGACATTATCAAGTAGTAGATGGTGACACGTTGTATTTTACCAATGATACTATAAAACAAGGTGTTAGATTTTTTGGAATAGATACTCCAGAGATATTTAAAGGCAAAAATAATCAACATTTAGCTTTGCTTGAAAACTATTATGGACAAAAAGCAAAAAATTATTTATACAATTTAACATATCAAAAAAATATAAAAATTAAATACATAACGAAGGATAAATATAATCGGTGAATTTGCAAAGTTTATAGTGCAGAAGGTAATGATTTAGCTTTTGAATTAGTTAAACAAGGATTTGCTAGAGTATCTTATATTTCAATTGATTATCATGCTAAATATTATTGAGTTAATAATAAAGAACAAAAAGAATATTTTTGAAAATTAAACCAGTTGCAAAATGAAGCACAACAAAATAAAAGAGGATTTTGAAAAGAAAAAAATATTAATTTAGTTTTTAATAAGAATTAAAAAAATACGCAAAATGCGTATCTTCTTGTTTGCTTATTAAGCAACAAATTCAGTAATACTTTCGTTTTCAACTACTAAAAAGCGGTCGCCTTTTTTAACACCATATGGTTTAAGAGCATTAAGAGCTTTGCTTTTATCTTTTTTGATTTTTGCAAATAATTCGATTGAATCTAATGATACTCAAACACTAGAAGTAACACCAAATCCACCGATTAGGTTTTCATCATTTAAAATACCAACGATTAATGTATTTGGTCTAAATTTAGCAACTTCACTTAATAATTGACCTGTTCTTGAAAGAACAACAGCAAATCTATAGTCACCATAACGTGTTCTGTTTGCAACGTCATAAGCAATTCTTGAACGTTTTGAATTTGAAGAATTTTTTCTAATTTCTTCTAATTGAACATCATAGTAATTTTTGTCATAAAATTCACTTTCAGCACGTTTGTTAATTTTTGACATAACTGTAACTGCTTCTAAAGGATAGTTGCCTGATGCACTTTCACCTGAAAGCATTGTTGAGTCAGCACCCATTTCTGTTGCATAGTAAACGTCAGTAACTTCAGCACGTGTAGGTTGTGGTGAGTTTTCCATTGAGTCTAACATTTGAGTTGCAACAACAACTGGTTTACCCGCTTCACGACATTTTCTGATCATTATTTTTTCAAAATAAGGAACATCATAGTAAGGAATTTCAAGACCTAAGTCCCCACGAGCAACCATAACACCATCTGATGCTTGAATGATTTCATCAATGTTTTTGGTTCCTAAGTGGCTTTCAATTTTAGAAATGATTTTAACGTGTTTTCCACCATTGTCATCTAATAATTTACGTAATTCTTGGACATTTTTAGATGAGTTAACAAATGATGCTGCTACATAGTTAATTCCGTTTTTAATACCAAATTTAACATCATCAATGTCTTTTTGAGCTAAAAATGGAAGAGAAAAGTCAACACCTGGTAAATTAATACGTTTGTTAGTTTTTAATAAGTGCTTGTTAAGTGTTTTAACAACAACATAACCAGCTTTTCTATCAACTTTAGTTACAATTGTTGATAATTTACCATCGTCAAAGAGAACTTGGTTTCCAACAACTAAGTCTTGAGCCATGTCATAAGCAACAGTTAACTCTTTTTCTGTTCCTTCTACGGTTTCATATTTTTCTTTAGTTGTTCAAACTTTAATTTCTTTGTTTGCTGGAATTTCTTGTTTTCCATCTTTCATTTTTCCTAAACGAATTTCCGGACCCTTAGTATCTAACATAAGAGATATAGGAAGATGTAAATCTTTAGAAATTTTTTGTGCAAGAACAAATTTGTTTAATTGTTCTGCATGGTCTCCGTGGCTAAAGTTAGCACGAACACAGGTTGCACCTGCTTCAATTAATGAACGAAGCATTTTGTAGTTATCACTTGATGGACCAACTGTAATAACTAACTTACTTCTTTTGTCAGTAATGTTCATTTATTCTCCTTAAATTAAAATAAACTTGTATGTATATATTTGTAATACTAAATATTATTTTATATTAAATTTGCAAAATAAATAACATATTTTTTTATTTGAACCATGATTAATAGTAAATTTGTAGTTTCATTATTTTCTTATTCTTTTTTATTAATAAAAAAAGAGCAATTTGCTCTCGATTTATGATTTTTTAAAACCACCTAAAGTTATATGTCCAATATATTCAGGAATTGAAGAAGACTTATAATTATTATTTGAATTTTTAACTCTAATACCAATTGCAAAGGTAGCTTCGCCTTTTGCTGGATCAAATGAAACTAAACCTTCATTTTTATATTTTGTTTTGTCTTTATCATAATCTTCTGTTTGTTTTGTACAATAGAATAATTGGTAAAGGTGACTATTTTTAAAATCATCTTTAAATTTCAATTGAATTCTTTTGTAGTAACCTTTACTTGCATTTTTGTATTCACCGAATTCATCTGTTGAATTATTTATTCATTCAAGTGAAGTTGAAGGTGTTTTAAAGTCAATATTTTTAATTTCATAGTTACCTAAAATAGATATTTCAACATGATTTTTCAATACTTTTTCAAGTTCAAAATAATTAATATCTATTTCTTGAACAATAGGATTTTTTATGTCAATCATTCTTGATTTTATTAAAGATGTTTTATCATTTTTGTCCTTAGGCATTTCCGCTTCCGGCACTATTTTATAGGCATAAAAGTGTAAATTTAATGATTTTTCAGAAGCTTTTGTAAATCACATTCCACGCTTTGTGTCTAATTTATATTCGGGGTTATTGCTTTTTTTATCTAAAGTTCTTCCAATGTCAAATCAACTTTCAATTGTTGAATTATTTTTTAAAATTTCTTCTTTATTTTTGTTTAAATCACTTGAAAGATCTTTTTGTGGTAATGCAGCAAGTAATTCATCAAGACTACTCTTTTCTTTCTTGTAAAAAGTTTGTCTTATTTTATCTCTAATATCCATTTTTCAAGATTTAGGATTACTAATTAATTTTGTGTTCGTTTCAACGACGTCTTTTTCAGTAACTTTTTTAAATCCATTTAAAACAAAAGTTTTTGATGTTCAGTTTTTTATTCTATCACTAATGTTTCTTGTTTTTTTATCTTTATCTTTTTCATTAGCTTTATCTTCTAATGAAACAGTCAAATAGAGTGTACCTGTAAAATCATTTGCATATGAATGGTAGAAAATATTGTAATCTTTATAAAATTTGTCAATTCTATCAAAACGATTATTTCTTAATGAAAATAATTGATTTCTCAATGCCCCTTGAGGAGAAAGATCATCATTTTTTAATCAACCTTTTTCAATATTTCCTTCAAGTTGAGTTCTTCAATAATTTTTTTCATATTCTTTTGTGTTTTTCAAAGTTGGTTCTTTGTTGCTATTGTATGTTTCAAAATAGTAATAATTTGAGTAAGTAAATTCACTTGCTAAGTGATTTGCAATATTAGTTTGGTATAATTTGTCTTTAGGTTTTGCAAGAGAAAAAGTAAAAGAGCTTAGCTGTCTTTCAATACTTTTATTGTCTTTATTAGTTTTAACTAAAGCATAAATAGCCGTACCAATTCCAACTGCAACAACACCTGTTGCTAATGTTCCTAATGTTAATGATAATTTACGATTCATTTTTTTCTCCTATTCAAATATAATTTTTAAATTATATATCAAATATTTGATATTAATGATTTTTACTCATTATAAAGTAATTTTTCTATGTGTTGAACTATATCATCAAGGTTAATATTTTTTTGGCTTGATACCAAGAACACTTGAGCTGATTTTTTAAAAATTTTTGAATTTAATTCATTATGTTTTTTAATTAAAGCCGATTTTTCTTTTTGATTTAATTTATCAATTTTTGTATAAACTATGTCATAATTTCAGTTTAATGAATTTAAAAATGTCAAGGTTTCTAAATCATTTTTAGTAAATCTTGTTCGACTATCTATAAGCAAAAAGATATGTTTTGGAGAAGGTTCATTTTTTAAAAAATCTTTAACCGCAAGATTCATTTTAATGACTTTTTCTTTAGATATTTCAGCATAACCATAACCAGGTAAGTCAACTATATATTTATCATTGTTATCTGCAAAATAATTAATTAATTGTGTTCTGCCAGGGTGCTTTGATACAAATGAAATATTTTGATTTGTTATAGCATTTAAAAGTGAACTTTTGCCAACATTTGAGCGACCTCAAAAACAAACTTGAAAGCTGTTTTTGCAATCAAGTCATTGTTTTTTATCTATTGCTGAAGTTATAAATTTTCACATTATTTTTTACCTAATTTTTGTTTATGTCTTAAAAGAGTTGAAGAATAGTCTATGTTTTCATAGTCTGGTACTATAAGTATAGTTTCAAGCTCAGAATTTAAGTGATGATTAGCAGCTGCTAGTACTAGCTCATAGTCATAATCTAAATTATTTCTGGCTGATCTAACTAGGAACTTTACATTTTTTTCTTTAGCAATTTGAGCTATCAAATCATTTTCATTTTTTACCACAGTGACTGTTTTATAGTCTTTTAAGGTTTTTTTGACTGATTGATATCTTTCTTCTAAATTACCTAAATTGTTTTTATCAGGGTTAATTGAAACTATTACTATAAGCTCATCAAAAAGCTTTAGTGCTTTTTCAATTACTGATATATGACCTTTGTGTATAGGATCAAAAGATCCTGGAAAAATTGCTTTTTTAATCATAATTATTTATACAAATTTATTGCATGTTTTGCAATTAATAATTCTTCATTTGTTCTAATTACATATACTTTGATTTCGCTATCTTTTGTAGAAATTAAAGCATAATCTTCAATTTTAGAATTATTCTTTTTGTCATCTAATTTAATCTTTGCAAAATGTAGTTTATCAACTACCATTTGACGCATTGAAGGAGTGTTTTCACCAACACCAGCAGTGAAAACAATAGCATCTAATTTGTTGCCAATTTTGTTTGCATAATTAGCGGTAAAATCAGCTATTTTTTGTGCATACAATTCAAGTGTGAAAATAGCACGTTTATTGCCTTCGTCTGCTGCTTTACATACATCACGCATATCGCTTGAAACACCTGAAACTCCTAAAAGCCCACTTTTCTTGTTAAGAGCTTCTGTGATTTGGTGAATATCTAAACCAAGTTGTTCATTAATAAATTCATGAATTGAAGGATCAATATCGCCTGAACGCGATCCCATCATAATTCCTGCTAAAGGTGTAAGTCCCATTGAAGTGTCAAATGATTTTCTATCTTTAATAGCACATAATGAAGCACCATTTCCAATATGTAAGTTAACTAAATTAACTTTTTCTTTTTTAACTAATTCTTGGATTTTGAGTGTAATAAATTCATGACTAATTCCATGAGCTCCATAACGACGAATTTTTAAATCTTCTGTTCATTGATATGGAATTGGATAAATTGCATTGACATCTGGAATTGTTGTGTGGAATGCTGTATCAAAGTCAACACTCATTTTGGCATGTGGCATAACTTTTTTAAATGCTTTCATGGCTTGAACTGCACCTGGATTGTGAAGTGGTGCATAAATTTTAGCTTTTTCAATTAAATCAATTTCTTTAGTATTCAATTTAACTGTTGTTGTAAAGTAAGGTCCACCATGAACAACTCTAAAGCCAATTAATTCGATTTCTTTTGGATTGTCAATTAAACCAATTTGTGACATTAATTCTAAAATGTTTTCAACAGCCACAGTGTGGTTAGGCATTGTTACATCACGGTTAAATTTTTGACCGTTATATTTGATTGTTATTTTACCTTCTAATAAGGTAATCCTCTCTGCAATACCACTTGCAATAATTTCTAAAGAAGCTTTATCAAAAAGTGATAACTTTATTGAACTACTTCCTGCATTAATAACTAATACTTTTTGCTTACTCATAATATTCTCCTATTTCTCTGCCTGCAATGCAGTGATTAAAATTGTATTAATTACATCTTTTACACTTGAACCTCTTGAAAGATCATTGACTGGTTTTTTAGTACCAACAACAATAGGTCCAATTGCCCCATAATTTCCTAATCTTTGAGCTAATTTATAGCCTATATTTCCAGCATCTAAATCAGGGAAAATTAAAGTATTTGCTGGTTCTTTATAGTAATCTAATTTATATTTAGCTTTTCTAACTTCTAAATCTAAAGCAGCATCTAATTGAATTTCTCCAATAGCTTTAGTACCTTGATAAATGGTATTGAATTCTTCAACAGCATTGTGTACTAATTCACTTTCAGGTGTTTTTGCTGAATAGTTAGTTGAGAAACTTAAGAAGGCTATTTTAGGTTCTATCCCCATGCTTTGAGCAAATTGAGCAGCATTAAGTCCAATTTCTACTAAAGCTTTTTGATCAGGCTTAACATTTACTGAAATGTCACTGAAGAAGTAAGTATTTTTTTCGTTATGTACTACCATAACTGAAGAAATAGTTTTTACTCCTTCTCTAGCGCCTATAGCTTTAAAAGCTGCTCTTAAGATATCTGCAGTTGCATAATTAAGTCCGCCAACTACCCCATCAACTTCACCTGTAGCTAAAAGCATCATAGCATAGAAAGGTCTTGTTTTTAGGTTTCTTTGAGCTTGTTCAATTGTTTCTTTGCCTTTTCTTAATTCAAAATATTTTTGAGCTAAATCATTTTGTTTTTGTTTATCTTTATTAATGTTTATAAACTTAACATTGGTTTTTGACTTAACATCAGTTTCAACTAATAAAGTTAATTCAACATTAGGAAATTGTTCTAATTGTTTAATGGTATCTAGTGCTCTACTATCATCACCATCTATAAAAGCAATTCTTTTTTTAGTTTTCATTGCTTTAATTCTTTTTTCAATTAAAGATGTAAATTTCATGAATACCTCCTATAAAAAAATATTCTTATATTAATTTTATATATATTATTAAATTTAATAATTAAATAATTACAATATGGCAATTTTTATCTTAAAAGGTAAATAAAAAGAACCTTAATTAAGGCCATAAACCAGGACAAAAGAATTAGACTTAAATCTCAAATATATTTAAGTCTTTTTGTACTAGTTTAAAGCATTAAATGAGGTTTTATGGGAAAACATTTAAATATTGAAAAATAGATAGAAATTTTTATATTTTTTAAGGAATATTTAGACTATAAAATTACAAAGACGGAATTTAAAAATGAATATTTTAGAATCAGTCAAAGACAAATATTTGATCGAGAAACAATTAGAAGTATCAAAAACAAATTTAAAAAATACAATTTGGGCATGAGTATAGAATCAAACACAGGAAAATCTCCTAAAAAAGGTAAAGGGTCTGGAAGACCTAGAAAAAATGGATATTGAAGCTGAAAAAAAGAATAGAGAAGAAGAATTAAGAAAGATGACAAGAGATCAACTTGAAATCTTAATAACTGATATTTCTTACAAAGAAATATTGGAAAGAAATAACAGTAAAGATTTAGATGAAGCAATTGAAAAATTCAAAAAAAGATATGGATTTTCTGTCACCATTAAACAAATTATCGCTATTTTAGGTATTAAAAAATCGACATATTATTATCAATTAAAACAAAGAAAAAAAGATTAAATATGAATATGAAATTAGACAAGCTTTTAAAGATTGTAAAGGTAGATATGGAAGAGCAAAAAGAAAAAGAGAAAATAAGAATACAAATGTTAAATATGAAAATCTATCTGAAAGAGATTATGATGGAAAACATAATGATATATTTGCTACTGTTGTAACATACATTCCATCTCCAATTGATGTTTTAGAAAATCATGTTTATTTATCTGCAATTATTCATCATAGAATTAAAAAAATAGTTAGTTGAAATTTATCAAAAAACAATGATAATAATCTCGTTTTAGAACATTTAAAAACAACTGATTTTTCTTCAAATTTTATTATTCATTCAGATCATGGCTATCAATATTCATCAAATGATTATGTAAAATTCATATCATCAAAAAATGAAAAAATCTCAATGTCAAGAGTTGGGAATTCATTATATAATAGAGAAATTGAATATTTCTTTTCTATATTAAAAACCGAAATCTTTCCTAATTTTTGCAAAAGAGTTAAGTTGCTCACTTTTAACGAATTGAAAAATGAAATTAAAGAATTTATTGATTGATATAATAATGATAGATTTATGAAAAAATTAGATCTAAAAACACCCCAACAAATGTGGGATGTTTATAAGAATAATAAATTTATTGGAGTCTAATTTTTTTGTCCTAGTTTAGTGACTTTAGTTTTTTAAACCCAAAAAGTTTGTTGAAAGCCCAAATTTTATGGTTTTTAAATTTATAAAATTATAATCTGTGAATATGTATTATTGATTTAGCTAAATTAGTAAAAAGTGAAATAACTGTCATTGGACCAACGCCACCTGGAACAGGGGCTAAAGCACTTAAATAGTTTTCAGGTATTGTTTCAATATCAACATCACCATGAAGAATTTTTTTGCCATCTACTTCAATTCAGTTTGATCCAACATCAACTACAACAGCACCTTCTTTAATGTTTTCTTTTTTAAGAAGTTTGGCTTCACCAGCAGCAACTACAATGATATCAGCATTTTCAACACCTTTAATGCCTGTGTTTCTATTGTAGGTAGAAACATTGCAACCTTCTTGTTTTAATAGAAATGCTGTAGGTTTTCCAACTAAACTTGAACGACCAATAACAGCAGCTTTTTTGTCAATCATTTTGATATTGTAGCTATTTAATAAATCTAAAATACCAATAGCAGTAGCTGGAACTAAGGTAAAGTCATCCGCTTGATTGTAGAAATTGAATGCATTTCTGGTACATAAACCATCAATATCCTTTTCAATGCGAACGCTATCTAAAATAACTTGTGTGGCAAATTCTTTAGGTAAAGGAAGTTGAATAACAATACCATCAGATTTTTCATTAATAATGTCTAATTTCTTAAGCAAATCTTTTTGTCTAATATCAGCTGGAAAATGATGATGAATTGCTTCTACTCCTAATTCTTCAGCCTTCTTTAATTTGTTTTTGATGTAGTGAGTAGATGCGGGATTATCGCCAACTTGAATAATTGATAAAATTGGTTTTCTGCCTAACTTTTCCTTTATATCCTTAAATTGTGCAATGAGTTCTTTAGTTCTTTTTTGAGCAAGAGTTTTTCCATCTAAGATTTTCATTTTAACTCCTCCTTGTATTTGTCTAATTCTTCTTGATATTTAGCTAGTTTTTGTTTTTCTTCTTCAACTTTAGATGTAGGTGCTTTAGCTATAAAGTTTTGATTACTTAAAATATTGTTTGAACGTTTAACTTCGCTTTCTAAATTAGCTATTTTCTCTTTTAATTTAGCAATGTAAGTGTTTTTTTGCTCGGCTGATTCTTTAATATAAACATTAATATTTCCATCAACAAATAAAGTGTCTTTATTTTCACTAATTTCACTGTTTGATAGTGATTTTAGTGAATTTTTAACAAACTCATTTTGTTTAATATTAGTGTCATAATAAAGCACTTCTTTTTTACTAATATTATTGTCTTCACGATATTTTCTAAGTATATTTACAATAGAAATTATTTGTTCAATTTGTTCAAGATTTTCAACTTTTTGATTCTTTAAGTTTGGAAATTCTTGAGCTAATAATTCTTTGTTAAATAGTACTTTATATAAGTGATCAGTAATAAAAGGTAAAAATGGATGAGCTATTAAAAGTAATGATTTTAGGTTATCTAAAGCAGCTTTTTTATTTGGCATTATTTTTAACAATTCAACATATCATGAAGAAAAATCATTGTAAATAAATTTAGACAATTCTGTACCAACAATTGTGAATTCATAATTTTGCATCGCCTTATCAATTCTCTTTTTAAGAGCAACTAATTTATTATGAATTCAAATGTCAGCAGCTGTTTTTTTATTTTCTTTATCTTCAGGTAATAATTGAATATAACGAGCAATATTTCATAATTTATTACATAATCCTCAAGCTGAAGTTATTTTTTCTTTTGAGTAACGAATATCTAAACCAGGGCTTGTGTTAGTAATCATAAATCATCTTAAAGCATCAGAACCATTTTCATTTATAACTTGAATTGGATCAACACCATTGTTTGATGATTTAGACATTTTTTTACCAAATTGGTCTCTAACAAGGCCATGAATTAATACTCTATCAAAAGGTTTTTGTTTCATGAATTCTAATCCAAAAAAATACATTCTAGCAACTCAAAAAAAGATAATGTCATAACCAGTTACTAAAAGTGAGGTTGGATAATATCTTTTTAAAAGAGTTGTTTTCTTAGGTCAACCTAAAAATGAGAATGGAGCTATACCACTACTAAATCAAGTATCAAGAACATCTTCATCTTGAACTCAACCTTCGCCAGGTGATTCAATTTGTACCTTAACTTTGTCATCTTTATATCAAGCAGGAATTCTATGACCTCATCAAAGTTGTCTACTTATTGTTCAGTCATGAATGTTTTCCATTCATTTTTTGATAACATTAACAAATCTTTTTGGATAAAAGTTAACTGCATCTTTTGATTTTAAGTGGTCTAAAATATCTTGAGCTAATTTGTCCATTTTAACAAATCATTGTGGCATAACAAGAGTTTCAACTGGAGTATTTGAACGTTCTGAATAACCAACATTTGAAGTAATGGTTTCTACTTTAACTAATAAATTATTATCAGCTAAATATTGACCAATTTTTTCTCTTGCTTCTTGACGAGTTAATTTGTGGAATTGACTTTTTGGACAGTTAATTAAACCGTCTTTATCAATAGTTTCATTAATTTGTAATTTGTGTTTTTTGATAATGTCAATATCAACTTCAGCATGAGCTGATAATTTCATTAAGCCACTACCAAATTTCATGTCTGCATATTCATCAGGCACAAATGGAATTATTTGTTTAGTTAAAGGATGAACAATTTGAATGTCTTTAAAATCTTTATATCTTTTGTCTTTTGGATTGTAAATAACAGCAACGTCACTAAGCATAGTTTCAGTTCTAACTGTGGCAACTGTTAAATATTTATTAGAATTCAAGATTGGATACTTGATATAGTACATTTTTTGTTCAGTAGGTTTATTAATAACCTCAATGTTACTTAGAGCAGTTTTTAAAACTGGATCTCAGTTTATTGCTTTAGTGCCATGATAAATTAATCCCTTTTTGTATAAGGTAATAAAAGCTTTTAAAACTGCTTCATTACTTTTAACATCTAAAGTAAATCTTTCTCTTTCATAATCTAAAGCTAAACCTAACGATGCTCATTGTTTTCTAAACAATTCAGCATATTCTTCTTTTCACTCTCAAACACGTTTAAGAAATTTCTCTCTTCCTAAATCATGTCTGTTTAATCCTTCTTTTTGATAAATTGTATTTTCAACTTTGCTTTGAGTTGCGATACCAGCATGATCCATTCCGGCTACATAAAAAACATCATAACCGCTTAATTTTTTGTATCGAATTATTGTATCCTGAATATATGTATCTAAAGCATGTCCAATATGTAATTTACCAGTAACATTCGGCGGAGGTAAAAGAATTGTAAAAGGTTTTTTGCTTAAATCATGTTTACTAAATATCTTTTTTTCTTTTCATTTCTTTGCTATATCACTCTCTATAGAAGAGTGGTCATAAATTTTTTCAAGCTCCATACTGCTCCAATCTTTTTTAACTAAATTAATTAAAAATTAAACTTTAAAAACTTAATTTTTTGTGCATATAAATGCATAATTATATATATAAATTATAATACAAAACCTTAATTTTGGAGGGCTCTTTTTAAGCAAAATTTAAAACTAATTTTTAATAATTGAGTAAAGAATTACTACTAAAGTTAGATCTTTTTATCATCCATTTTTAATCCCTGTAATTTTTCAAAATCACTATAAAACAATTGTTTTAAAAAATAAACCAACTTTAAAAATGGTCTATTTCTTAAGTTCAATAAGCATATTAAGTTCCGTCTTAAATGCTTTTTATTTTTTGTATTTGTTTTCACAAACTCTTGTGATTCAACCAATGAATCAGAAAATTCCATGAATTGCATAACCTAAAACAAAATTAAATATTAAATATAACAATGTAGCTACATAATAATTTTTGCTTCCGTTAATATGTAAAATATTTTCAAACATTTTAACAAATGAAATCTCTTGACGATGACTCATTAAAAAGAAGTCAGGTAAATAATTAGGGAATCATTCTAAGTTATTTTTAGCTGTTTGATAAACAATTTCATTCCCTATAACTGCAAGTGACCATAAAATTGTAAAGATAACAAAACTATTAACAATAAACATTCCCTTTTTGTATTTTGCTCAATTATTAACAACTAAAATCATACCGATAGCTAACATGCAGCCATGATACATCATTGTATGGATTGAAATTAGTATAACTTCATGAAATACATCGCCTGGTCAAAAAATCATAATGAATGCTCCACCATAAATTGTTATGTAAGCTAAAAAGTCAGTTATGATTTCTTTAAATTTAGTTTTCTTTCTTAAAAACAAATAAAGAGGTACAAAATATAAAGGAAGTGAACATATAACAACTGGATAAAGGTTTTGATGATAATCTCATTTCCATTTATATTCTCCTGCTGTATTTTTGACAGCTTTTCCTGCTCATAACATTTGTTTCAATAATTCCATAAAGACTATAATAAAGAATAAAAGTCCAATAGTTAATTTAACTGTTCATTCTTTTCTATTTCTTAAAGCAACACATAAAATGATGCCTAAAATAAGAAATGTTGCTAAAAATGTCAAGTGGAATGCGCTAAAAGCTTCTGGTCTTCCATTTGCAAATTCAGTATTAAATTTTAAATAAAATTTTGACATTGTATCCATGCTTAAAATTATACAAAAAATATTGAATTTGTGTCCCACAAACCTTCTAGGTTTTAAAACATTTACTTTTATGTAATATAATTAGTAATATATAAAAATAAATATAGGAGTTAAAAATGGCAGAAGCAAAGAAAAAAACAACAGCTGCAAATAAAGCTAAAAAACCAGTAGCAACCAAAACAACAAAAAAAGTTACAGCAGCTAAAACAATTAAAAAACCAGCTGCAACAAAAGCAAAAGTAGCTAAAAAACCAACATTAGATAAATATTATATCTCAGAAAAACCAATCAAAAAAACTGGTGATATTGAATTCAACTTTAAAAAAGCAAAACAAAAGACATCAAGAAAATATGATGAATTTATCGATGCTCTTAAAGAATTTATTTTTGTTAGCGAAACATCGAAAAATGATACAAGAGTTTGATTCCATAGAAATGGAGCTTACCGTGGATCAGTAGATTTAGCAAAAGCAAAAATAATCTTAGATCGCCTTACTCAACAAGCAGTTGAAAAACAAAGAATTATTGAATATCTTGAACAAGAAAATCTTGTTGATAAACCAGAACCTAAAAAAGTAACTAAAAAAGTTATTATTGTTGAAGAACCTAAACCAATTGATCTTAATCAAGAAGCTAAAGAAGCAACATTCTTTGTTCCTAATTCAGATTTAATGTTAGCTTCAGAAGTGCAAAAAGATGACATTGTTGCTTCAACACCAACAAGAGGTTGTGAAGTTTTTGTAACAAACATTTATCCAAGCAATGGACAAATGGAAGTTGAATATGTTGTTAAAAATGAAAACAATCAATCAGATTCTCAAATCAAAACTTTATATGGATTTAAACAAAATCCAGTTACTGAAGAAATAATGACTATTTCTGATGAAGAAGAAAAAAAATTAGAATCTAATATTGTTGAAGACACAGAACCTGCTGAAGTTGTTGATGTAGAAAGTGATGAAAATAAAAATACATCAGTTGAAAATGACAAAAAGTCAAAAATGTCAAAAGAACTTTTATGAACAATAATCACAATGTCAGTATTGCTTATTGCCGCTCTTACTATCTTAGTTTTAGTTGCAACCCGAGTAATTTAATTAATTTTTTGAAAATAAAAATGCTCTTATTGAAAGGACATTTTGTTTTTGTTTTCTGGTATCTTATTTGATACTTTTATAATTATAATTAAGCTATATACAGCTAAAGCTAGAACAAAATTTATTACTGGATAAAGCAACGTAATTACTCAGTAGTTGCCATTTAATTTAACACTAAAAATTTTCTCAAATAATAGTGTCAAATGATTATTAAGATGATGACTAATTGCTAATAAATTTGGATAATTTTCTTGTAATTTATTTAAGTCTTTGTGTGCTGTTTGATAAAAAATTTCATTCAATATTACTACTACACTAAACATTATTAGGAACATAAAAAGATTATGAATAATATAATTTCCTTTGTCGAATTTTGAATAATTATTAAAAATTAAAACAATACCAATTAACATTAAAATTCCATGATGAATCATTGAGTGACAATCAAAAAATATCGATTTATTTAAGACTTCATTTGGAATAAATATCATTATTGAAATTCCGCCATAAAGGCTAGTAAATGAAACAAAATCGATCAAAATTTTATTTAGATTTTTGTTTTGATTAATCAAATAAATAGGAAATAAAAAGTAAGGAATTGAACAAAAAAATAAAGGAAAATAAGATCATTTATATTTTGAATATCATACATTATCTTTATCTATTTAACCTAAAGCACTTAGTTGTTTTATTATTTCAAAAGTTACTAAAATACCTAAACAAATCAAAATAAATAGCTTAACTTGTTTATCATTTTTGTTTTTAAATTTGTAACAAATGACACCAGTTAAAATGACAAAAATACCAATCACGATAAGTAAAATCATCCATAAGCTTGTGGAGTATTTGAGAAGGATCACGATATTACTTTATAAAAATTTGCCATGATTTAACAACCTTTGTTCTAATAATATTTTACTAAAAAACCTATAAAACAGTTTTATAGGATTATATTAATATATGTCATCTGATAATACTTGAGTCATTGTCAAGTCAGTTTCAACTTTTTGATTACTATTATTTACTTTTTGAATAGCTTCTAATTCTTGAGTAAATGTTTTATCCAAATTTTTGTTATTTTTAATTTTATTAGGCTGGAAAGCAGCTATCATAATTGTCTTTTCTAATAAAGGATTCTCTAAATCATTTATTTGTGTTTCAATAGTTTTTTCTTGACTCAATTCATCAGTTACTAATAAAGAATTATTTTCATTTTTATGGCCTTGCATTCATAATTTGAAATATTTTGAAGGTGTAATAATGTAGTGCTCTTCATCTACATCATAAATATCACTAAAAACAAATTCTTTTTGACTTCTAATAATTTCTTGTGAAATGGCTTGGTCATTAATTTTACTTTTAATTTCAGGGTTAATTGTGTTATTTAAAACTTCTTTTAAATCTCCAAATTCAACTTCTTTATTATTAGCAATCAAATAGATATAGTCAAACTCATTGTCTTCAACTAATTCTTTATCTTGAGTAACAAAAATAATATTTATTTTACTTTGTTCACATAATTCAAAAGCAGCTCTTAAAAATTCAAATTTAAGTTTTGTATCTTTAATATCAGGATCATCAGCAATAATAATTAGTTTTGGTTTCTTAATAATAGAAGAAATAAAGTTCATTTTTAGTTTGTTAATTTTTGAAATATTTTCATAAGGTCTAATTAAGCTCTTTGTATTGATTGATGATTTTTCAAAAATTTCAATTAATCTGTCAAATTGGTCTAATTTGTGAAGAATTTTATTAGAAACTTTTTTAGCTAAAACTAAACTATTTTTAAGAACAATAACATTATTAAATACAAAGTCAAACATTTCAAATTTTTCAATTATTTTCTTTAAATTAGCACTAAAATTTTTAATGTTTAATTTGTCTTTTTCATTAATTGAACGATTAAAAATGCGTTTAAATCACAATGAAGTTTTTAACTTCATGATTTTTCTTTTTATTTTTGAATTAATCAAAGTCATAGTGTCATAACTGTTTAATAATTTTTGATTTGATTTTAGTGTGTTATAGAATTCTGATTGAATTTTGTTGAATTTTCAATTATGTTCACTTTGAGCATTAGTTAAAATTTCTTTTGCAACTTTGATAGATCTTTCAGGGACAGTATTGAAGTATGTTCCATATGATCTATTTTTAATAGACATTATTTGACTTTGAATTTGATCTTTTGAATCTTTAATTAAGTCAAAATTGCTTTTTGATTTGATTTTATAACTTTCGACATTAAAGTAGAATTCTTCTTTAAGAATCTTTTTAATTTTTTTAATTGAAAAGTTTTTATTGCTGTAAGTTAAATTTCCTAAACTATTGCTAATAAATAATTTGATGGTGGTATCTAGTGTTCTTCTCATTTCATCAAGTTCATCAATTTTTAAATATTTAAAATATTTTTTATATTTTTTAAGTAACTTGTGAGCAAACATTTTAACAAAATATCTTTTGTTTAAAATTTGTCTTTGTGAGCTTTCTTTAGCAAAAAAAGTTGATTTATACAAATCAATTTCGGCACTTCTTTTATATCTAAAAGCAAAGTATTTAATTTTTTGATCTGAGTTGTGACAAATTTCTTTTGCTATTTCATCTAAAGCACAAATATCATGTTTTAAGTCTCTAATTTTTAAGTTGTCTTCCACTCTTTTTTTAGAAGTTTTTCTCATTTTGGTTAAGTAGTAAAGTCTCTTTTTTAAGTGATTTATTTGTTCATCACCCAAGTAGTCAATATTTGATTTAGATGCATTATAAAAGAATTCAAATTTATTAAATACTTGAGAAAACAAGTCTAATATTAAAGTAATTTGTTCACCAAAAAATAAGTCTTGGTAGCTTTCAATTTCTTTAAATCTTGTTTGTAATTCATCAAAGTTATATTCAGCAATATTTTTTGTTGTTTTATTTAAAAAACTATTGAAGTTTCTTTCAAAATCACGAAAAATCGCCATTAATGAGTTAGCTTGTTCTACTAAAATATTCAAAATAACATGACGAACAGGATAATAGTATTTATTAACTGTCTTATTTAATTGGTTAATACGTTCCTTGTTTTCACCGTAATTTTTTTTAAGTTCTTCATATTTGTAAACTAAAGGATATTGTAAATTTTGTTCTTCTTTTTCAGCCATATTGAAAAAGTTAATTTCACTAAAAGCATTATTATTTTTATTGAATTCAATGTTTCAATAATTTGTTCCTGAACCTTTTTTATTATTCTTAGAAAAGAAAGATAAAATGTTCAAATTGTTATTGAAAATTGTATTGTAAAAATTGGAACCAATTAGGTTTCTGTTTTTATCAGTAACTAAAATTGCAGTTTTAAGTTGATCGTAAATCTCAATTTTTTGTAAATCTACATAATCATCTTCAAAATTTAGATTCTTAAAACAATTTTCAATTCCAAATATTTTATTTTTCTTCATATTAATAAAAAGTATATTAAAAAAATGACATTTTTATAGCAAAATATTATATAATATTTTTTGCATGGGGGTGTAATGGCTTCGACATGCATTGGGTGATACTAATATCAGTAGTTTGGCAGACTATAATGCATCTAGGCTTTATAATCGCAGAAGATAAAAAAGCAGAAGAAGTTAATATTTCTTCACTTATGATTGCACAAAAAATGCAATCACAATCAAACCTTGCTTTCGCTTAGTTAAAAGTGACAAGTGGTTTTAAAGTTGACATTTTCCTATATATTTTAAAATCGGCTTTTAAGGAGAACAGGAGTCTGAAAGGGTTCCAAAAATCTATATTGTTTGCATTTCGGTAGTATAGATTAATTAGAAATGATAAACTGTAAAAAGTATTGGTATTGACTTGGTGTGTGGACTCGGGTTCAACTCCCGACATCTCCACCATATGCTGGCTGAGCCAGCTTTTATTTTTTTAAATTAAGCTGTTTTATAGGCTTATTAGTCAAAATAATAAATAAAAAATATGTTAAAAATTTTTAATAAAAAATTTATAATTTATTTAAAGGAGGCTTATGGAAGGAATATCAGAAGAATTATTAAAAGGCAAAAGTAATGTTATGTCTTTAGGCAAATTGAACAATATAATTAAAGACAATTTAAAAAACAACTTTTGACAAACTTATTGAATAAAAGCTTCTATTTCTAAATTAAGTATTAAAAATCATGCTTACTTAAATATTGTTGATGGCGAATATTCATCTAATGCTGCAATATGAGCAAGCAAGATTCCGAATATTGGAATTGATATAACAAGTAACAATAAAGATCTTTATAGTCATGCTTATTTTATGAATGTTAGCATATCGCCATATTCTTTAAATAATTCAGTTTCTTATTCAATTGAACAAATTATTGATTTAGATACTTATTCAAAAGAGAAAATTGAAAAAGAAAAATTAAAGAAACTTTATTTATCAAAAGGTTATTTTGATCCTAATAGAAAGAGACCAATTCCAAAATACTGTGAATATATTGCGGTTATTGCTGCAGAAAGTGGTCAAGCAGTTCGAGATGTTTATACAACTGTTTTATCAAGATTTCCAATTGCAAATTTAAAATTATTTAATTCATCAGTTCAAGGCCAACAAGCCTTAATTGATATACCGAAGCAAATTGCAAATGCTAATAAAGAAAACTTTGATGTTATTATTATTGCTCGTGGTGGTGGAAGTACAGTTGATTTAGAAATCTTTAACCAAGAAGCAATTGTTAATGCTATTGTTGAAAGTAAAATTCCAATTGTTACAGCTATTGGTCATACAGATGATATTAGTTTTAGTGATTTAATAGCAGATGCTTATGGTAATACTCCAACAGGAGCAGCAAAATTAGTTGTGCCTGATAAGGAAGAAATTCTAAGTTTATTAAGAAACTATCGAGCAAGAAATCAAAATTATATTAACAAAATGCTTTTGGATAGTCAAAATAAATTAAAAAAATTGGCTCAAAAAATTTATGAATTTAAACCAGAGTTAATAATTGAACAATATGAAAATAAAGTTAATAAATTTAAAGAATTTATAAAAAATCATAAAATTCTTTTATTACAAAAGTTAGAAACTAAAATTAAAGAATTTAAATAATTAGTTAAAAGCAGCAAAGAATTATTCTTACAAAAAATACAAGCAAAATTTGAAATGGCTAAAACGAGATTCAAGAATCAAGCCTTGCATTGTTTCAATAATCAATTAGCAACTTTTTCAATAGTAAAAGAAAAAGTTATTCAACGTAATCCATTAAAAATATTAGAAAAAGGTTTTGCAAAAGTTACAAATGAAAACAACCAAATAATTAAAACAATAAAACAAGTTAAAGTTGATAAAAAACTTTACATTGAATTAAAAGATGGTATAGCTGAAGTAGTTGTACAAAAAGTTAATAAAAAATAAGGAGAAATTATGTCAGAAAAATTAAAATTTAATCAAATCATGAATCAAATAGATGAAATTAGCAACAAACTCGGATCAAACATTGATTTAGAGGATTCCATTAATTTATACAAACAAGCTGAAAAATTAATAGAAGAAGCTGAAACAAAACTAGTTGAAGTTAGAAAAATCGTTGCTAAAGAATCTATAAAACAATAATATTAGTCAAATTGAGTTCTTTTTTAGGACTTATTTTTTTAACTTTTTAGAGCTAATTTTTTAACCATTTTTTATTTAAAAAATAGTTATTTTTTAATATTAAAAATTATTTTAATTATTTACTTATTTATAACTATTCGTTTATATATAATAAATAAATATCAGGAGGGAAAAATGAAACAAATTTTTGCAAACGATACAAACTCTATTAAAAGAATTGATGTTCAAAAAATGTATGCATCAAAACATAAAAAATTAGTTAATAACCTTTTAGATATTAAAAGTAAAGGCGATAATGCGACATTTTTTAAGACTTCAAATTTATCTTTAGATCTTAAAAAACTTATTGGAATTAAAAACTTAAGAGAAATTTTAAACAATGTTGAATTTAAAATTGCACTCAAAAATTCTTTTGATTCTAAAAAACTAAATGAATTAAAAAACATTAATACAGTTAGTCAATTTGAAGTCTGAACTAAAGAAAATGATGTTCAAATTACACCAAAAATGGCAGAAAACTTGGCAAAAGATTCTATTAAATGACTTAATGTTTATAAACATAGAATATTAGATGCTAATGGCAACTTTATTAAAACTTGAAAAAGCAATGCCAAAAGAGTTGAAGAAATTTATTCAGAAACAAATATTTGACCTTTATTTATTGGGACTTATTTTGTTAAATATAGAAAAGATGAGGTTTGTTTATATGCACCTTATATTTTAAAGGCTGTAAAAATTACAATTAGCAATCAATCAGTTTTCTTAGAAAATGCTGATCAAAACATCATTATTAATGAAAAAGTTAAATATTTACTTGAACAACACAGTCAATTTAATTTACCTATTTTAGTTGACGGCGAAGAAGTTACTTTGACTGAAGCATTAAACAGTATTAAAAATATAGACAGTAAATATTTGAACAATTTAGATTATAAAGAAACTATATTCCAAGAACCATTTGCTGAATATTCTAAAGTCCAATTAGAAAATAATGGTTGTGTCGAACTATTTCCCGGAACTGTATTAATTGAAGCAAATCCAGGCGGTACTAAATTGCGTGAAGCAATGATTAATTTAATTTTAAATAACAAAATAGACAACTTGATTGAAATAGATGACTTAAAAGATTATACTAAAACAGCTTATGAAAAAGCATTAGGCAAAGGCAATATTGCACGTGTAACACCATCAGATTTTAGTCAAGAAAAAGCAATTATAGGAACACTAAATAATTCAGCAATTATTATTGGTCCTCCTGGTACAGGAAAGAGTCAAACTATTGCAAATATCTTAGTTAACATTGCGAAAGAAAACAAAAAAGCTTTATTTATTTCTCAAAAGAAAGTAGCTTTAGATGTTGTTTTAAAACGTCTTGGAAAATATCAAAATATGATGTTTCAATTCTCTGAAACAGCTAAAGTAAATCAAAGTGAAAAAGAATACTTTTATAAACCAATTATTGAGTATTTTAAAGAAATTAATGAAGTCCAAGAAAACCATGATATTTCTAAATATAATTCAAGCTTTTTCTCTAAAGGAGAGTTCAAATATTTAGATGCAAAAAGTGAAATTGGTGAAATATTTTCAAAAGACTTAAACTGTTATTTTAACCTAAAAAATAAGGGAAAAATTGAACAAAATATACCAAAATTTATTCAATTTTATAAGTTATATAATGAACTATTTTCTAAGAATAAATTTTTAAAAATATTCAACAATTATAAAACACTTTCAAAACGCGAAATAGCCTATAAAACAGGTTATTTGAAAAAATATGCAACTATTTTTACTAAATTACCAGCAGAGTTTAAAAGACTATGAAATGTATCGAAAAAAATCATTAAATTCTACAATAAAAATTTAAGTAGATATGACATCAATGATCTTGTACAAATGTCAAACTTTATTAGACTTAATTCATTTATTGAATTTGATAAAAATGAAAAGGATTACAACAAATTTTTAGAAACTAAAAAGTCAGATAATACCACACCTGAACTATTAGAAATGATTTATGAAAATAATGCTTTTGAAGCTAAAAAACGTTTAGATGACTATCGTCAAAAAAGTGAAGCACAACGTCAAAAATTAAACAAATTTTTTGGACGTTTAGAACGTAAAATTACACCTCCTTATATTATTAGTTCATTGTTTAAAGATATCATTAAAAATGTATACAACATTTATGTTGGTACACCTGAAATTTTAGGTAACTTTATTGATTTTGAAAATGACCATTATGATTATGTAATCTTCGATGAGGCTAGTCAGATTTACATTGAAAAAGCCATTCCTTTTATTTCAATAGCTGACAAAGTTATTGTTGCTGGCGATAATCAACAAATGCAGCCAAGTAACTGATTTAATACTCGTGATGAAAATGAAGATGAATATAGTGAAGAAGAAATTACCTCATTATTAGATTGAGCAATTCATAATCACTTGCCTAAATATTTACTTGAAATGAATTACCGTTCAAATGCTAGTGAGCTTGTTTTATTTTCTTCAAAAGAATTCTATGAATCAGGCCTTAAAGGTTTGGATAACTATCCAAGAAAAGAAAAAACTAGTGTTGAAGTTATTGATGTTGATGGTCAATGAGTTGACAATAAAAATGAAGTTGAATGTGAAAAAATGATCCAAGTTTGTGAAAAGAATATCAAAAAATACAACTCAATTATTCTTCTAGCATTCAATAGAAAACAACAAGATTACATTCGTGAACAAATTGCCTTAAATAGTCCAAAAATCTTTGCTGAATTAGAAGACAAAGTTATCTTAAGAAACATTGAAAACATCCAAGGCGATGAAGCAGAATTAGTTATTGCTTCAGTAGGTTATACTGGTAAAACTGCTTTACATTCAACTTACATTGGTTCTAAAAAAGGTCGAAATGCTTTAAATGTTGCTATTACTAGAGCTAAAGATAAAATGATAATTATTAAAAGTATCAAAACTAGTGAAGTTGATGCTAAGAGTGAAAACTTGATGACATTTAAACGTTGATTAAACTACATTCAATTAAATGAAAACAGACAAAAAGAATACGCAAATGTAGTAGCAGGCTTAATTTTTAATGATAAAAGCCAATTTATGTATGAGTTAGAAAAATGAATTAAAGAACAAAACTTTTCAAATGAAATCAAAGTTGTCCAAGATTACTGTGTAGGTTCTTATAACATTGATTTAGCAATTTTGGATAATAAAAGCCAATTTGTAGTTGGCTTAAATGTAGATAGTTCAAGTGATGACATTGAAATTGATGATTTCATTGAAAAAAGAATAAAAACTGATTTTATGTCAGTTAAAGGATACCCAATTTATCGTGTTTCTAACTTTAGATTTGCAGAAAATCAGCCTTCAATTTATCGCTTTTTAAACACTTTAATTAGTAAAGCTTAATTATTAAATTACTAGAGTTTTATAGTGCTCTAGTTTTTTTAATTTGCAATTTTAAAAAGTGAAATTTAGGCCTTAAAAAATATCTTTATGTATATTTTTTGCTTGTCTTATTACCTTTTTAATATATACTTTTATTATTGTTATAATAATAAGGGACAGAAAATGAAAAAATTTGTTGCTAATTTTGTATTAAACAATTGAGGAGGATTATCATCAAATCCTGCTTTTAGTAACAAATATGTCGAGTTCATTTATCAAAAGTAATTAATGCTTAAATGTGCTCAAAAAAGGCACATTTTTGTTTGCTTTCTAAATAACAATGCAATATCAACGAAAGGAAAATATGAAAAAACCAAAATTCAATTTTCGTACTCATGTTTGACCTAAATATGTAATTTCTGCAGGTGTGCTTGCTGCAGCAACTGCAATTACATTAGGTTCACTTTATGGATGTGCGAAAAATTCAGATGAAAAACTTGGCAAGGCACATACGGGTAATCCTAAAGACTTTAAGAATTATTTTGTTAGTGTTGATAAAGCTGAAAGTAATTTTGTGACAACAGACAAAAGTAAAAAAGTTGCAAAATTTGACTTTGCTAAAAATCAAGTTATTAAAGAAAATGGTCAAGCTATTAGTGTTGACGAATTTTTAGATCAATATTATCGTGAACATCATAATCTACCATTTTTAAACATTAAATATGGTTCATTTAATTTTTACAATGAATATATTGAAGCAGTTAGTCCATTAGAATTTCAAAAATTTACTAACTGATTTATGACCAATGTTTCTTGAGGCCCAGAAATTATTACTCTTAAATCATTCTCAATTGTTAAGGGTGTTGAAATGCATGGAAATAGCATTACTTTAGGTGCTCACTCAAGTAAAAATAAAGAGTTAACTACTATTAAATTCTACCCAGATGCTTTCTTTGGAACACTACCAATTTACTCAGAATTAAGTGGAGCTGGTAATGCTCAAGATTCTTTAGTTTATAAAGTAAACCAAAAACTTTTAACATCAGAACAGTTAAAAGATTTTTTGGCTAACATTTCAAATTACAATGCAATTAGTAATTATTCAAAAACAACTTTAGACAATTATGGTTTTAGAAATCTTCTTGATTATAGAACATTAATTAATCAAAAAGTTTGAGTTATTGAAGGTAATTGAAAAGAACAATTAAAACAATACACCTTAGATAATATTGAATTAAGCAGATTAATGTGAGCTAATCCATATTTAACTTTAATTCAAGGAAAAGATGAAGAAACTGCTAGAAAGAATTTACTAATTGCTTTAGAAAAATACAAAAATACAGATGAATTTAACATTATTAAAAACATTGCTCAAAATGTTAAATTAGTCAAAAACTATTACTAATATTCATAAAGGTTCCAACCCTATTGCTAAAAATGCAAATATTATGGATAGTTACTTAAATATTACTTTTGAAGATGGAACAAGTTTTGATGTTTTTAATAATTTTGAAGATATTACTCATAAAAATCCTAAAAATGAATGAGTTAAAAATGATAAAGTTGTAAATATTGCTCGTGGTTACAATGAATCACTTGAAAGAATTAACTTATTAAAAGAAGAATTTATAAACAATATTAGAAAAATTGCAAACAAAAATGTTAGCGAAGAAGAAATTAATAATTATGTTAAAGCGGTTATTGCAATTCAACCAACTATTCAAGAAATTACTAAATTAAATGAAACTAAAGCTCAATTAGATGAAGAATTTCAAGAACAAGAAAACTTTAAAGCTCAAGTTGCAAAATTTGAAGAAGAAATTACTGATAAACAAAATAAAGTAGTTCAAAAAGAAACTAAGCTTAGTGAAGTTAAACAAAAAATAAATCAAAAGAATAATGAAATTAAAGATTTAGAAGAACAACTAAAAAGTGATCCTTCAAATCAAGACTTAAAAGATAAACTAGCAAAAACTAAAGAACAACAAGAAAAACTTGTTAAAGAAAGAAATGAATTATTTAAAGAAATTAATAACTTTAAAAACAATATTAATGCAACAAAAAAACAATTACAAAATGTAAAAAACAAGCTTTGAAAATTTATAACTCAAAAAGCTAAAGAAGACAAACTTAAAGAAACAAAAGAAACTATTGATGCTAAGGTTAAATCAACTACTGAAGAAATAGAAAAAGATTCAAAAATTGAAAAGAATCAAACACCTGAAGCCTTAAACGCTTTAAGAAGTTTAGCACAATTAATTCATGATTTTTATGAAGAAAAAGATGTTAAATTACATAAACAATTAGATGAAAGCACAGAAGATTTAGTTGCTCCAGCAGGCAAAAATATTTATACATACAAAAAAGAATTAGCAAATAAATTTGACCTAATTCATTTTGAATATAATGAAAAAATCAAAAAAATTACAACTCCTTATTTTGATGGAAAAGCCTATGAAACAGTAAAATTTGTTCCTTACTCATATTACACAACTGATATTGCATACTTACCAAACCAACTAATAGCTATTGACTCATTAGTTGATATTTCACGTCTTCAACAAATTAATTGAAGAAACTTTTATAACTTAGATGGTTACATTCGCAGCAAAAATATTGATGAAATTAGTAACAATGATGGCTTTTATGTTTATACAAAAAATGTTTCATCACTTGAAGAAGATTTCAACAAAAATAACCCAGATTTAAAATACAAAATAGAATATTCAAAAACTATTAAAAATAGAAAAGAATTACAAAACAAACAAAAACAATTAATGGCTTGAAATACTGAATTTACTGAATTGATTTCAAAAAGAATTAAAGGTTTATTTACACCATTTATAACTAAAAATGAAACTCTTCCAGAAAACTATTTAATTTTAGGAAATCATGGTGATTACCCTCTTACTAAATTTAACATCATAGAAAAATTTGATCAATATGATTCAAAAAATATACTTGAATACATTGATATTGTTTACAATGGATTATCAACAGCAACCAATCCTCATCATAAAGAAGGCTACAAAAAATATTACACTAGAATTGAAGAAAAAATTAAAGCTGGAGATGCTACAGCTTTTAATCTTTATTCACAACTTAAAGCATTGCAAGTTAAACGTTTTGAGATTATAGAAAAGCAAAATGATCCTAAAGAAGACAAAACTTTATTGCAAGCTCAATTGCTATCAATCACTTATCAATTTGACCAATTGAAAAAATAATGAGAAGAAACTTACAATAAAGTAGCAAACAAATCTTGATATATAAGTACAAATCAACTTATTTTGTTATGTAAACTCGATTTAAATCTTCAAACTGCGAAAAAAATAGCCGAACTTCATCAAAGTTTAATTAATGAATACAAACAAAAAAGACTTGAATTAGAAACAATTGAAAGAAAAATTGAGTTAGAAAACTTCAAAATCCTTTATATGACTCAAACAGCAAATTTTGCCGATCCTGTTGTTATTGAACAATACAATAGAATTCTTCATAAATTTGAAGAAAATATTAATACTCACATGCCGACTTATATTGCAAAAGTTGTTGATGAACAATTCAAAGCAGCAGATTTAAAAGGAAAACAAGTATTAGAAATACTTAAAAATCCTACAACTTATAATCAAGTATCAATTTTCATTTCGGAAATTGAAAAAACAAATCGATACTTTAAAGACTTTAAAAATAAAGTGTTATTATCTCAAGCTGAATTAGTTGCTAAAATTCAAGTTGAAGTTAATTCTGATGAAAATTTATATAATCTTAACAAAAAGTTTTGAGAAGATCTAAAAGCAGAATACACACCTTTAATTAAGCCTTACAATGATGCTTTTGAAGTTGATATGACTTATGAAATTGAGGACAATAACAACAAAAAAATTTCTGTTACTAAAAAAGCAAAAGAAAGAATGTTCGATTCTTATGCACAAGAAGAAGATGAGTTGAATCAAGTTGCAACACATTTAACAAACAGAATTAGCACTCATCAAACTGTACTTGACAATATAAATGCAAAAATAAATCAATTACAAGTTGAATATAAAGATCCTAAAATAACTGCTGAAAGAAAAGTTGAAATAATTGAATTACTAAAACAAATAGCTAAACTTTATGACAATACCAAAACAGCTATTTATCATATAAATGATGTTTTAAGAGAAATTAAATTAGCAATTAGCAATTTATTAGAAATTAAACAAGGCATTACAAATGGTACGCACACATATTTGGATGCTTATGATTTAGATTCTAATTTATTGAGTTGAATTAATGAAATTAATAGTAAACCAATTTGAAATATTGATAAATGAGTTAAAACACTTATCAATGGTCAATTGAGAAGAGCTATTAGAGAACGTACTGATTGATTTAAAAAGGAAATAGAACAAAAATTAAATATAGCAAAAAAAGCTGAAAAATTAGATGAACTCAAAGGAAAAATTAAAAAGGCAGAAAATGAATTAGCGCCTATTAAAGAACGTTTTGATACTAAAAAAGCAGTTTTGGATGTTGCTAATAAAATTGAAACTTCACTATATAAAGAAGCAATTAATAAAGAAATAGAATCAACACTTGCATATAAAGCAAGCGAAAATAGCATTAAAAACCAAATTGAATTTTTCTCTAATTTGATGCCAGATTATTCAAAAATTATTAATGGTTTAGCTAAAAATTCAGAAATTGGTAATATGATTCTCGGTTTAAATATCGAAGTGGAAAAAAGTTGTGCTAATGATCCTTTAATTAAAAAATTAAACAATGTTTTAAAAGACTTAGATGATCCAAATAGTGAAATCAATAAATTTAAAGAAAAAGCCCAAAATGCCTATAAAACAGCAGGATTGAAAGATCAATTGGATAATATTGAGTCTGAAAAAAGTAAATATGGAAAGATGACTGATTCATTTATTAAATTGTTAAATGATACTATTAAAGTCAAAATTTTATCTAATTATATTAAAAATGAAAGTATCGATATTGAATCTTATGTTAAAAATACAAATAATGAGGCAAGACTTGCTGATACAAAAGATGCTGTTACTTGAATAATAAAAAATGTAAAAGGAATTCATTACCAATTAGAATTAGATAAAAAGGCAAAATTAGAAGAAATTGCAAATCAACTTAAGAAAATTAAAACCTTAACAATTGATTTAATTAAAAAAACAGAAGATATTCTTGGCCACAAAGATGTTGTAAATTGCATTGATAAAATCAATTCATTATTCAATCAATTTGTTATTAAATCATCAGGTTTAATTTCACCTAAATATATTAATGATCCAGTGATTAAAGCTCATTTGGAAATTATTGATAAAAGTAGAATTGAAGCAATTGAAAAAAGTATGCCTAAATTAAATCAAGATGAAAAACTTGTTAAATATGAAGCAATTATTAATGAAGGTTTTCAAAAAACTATTGATAAACTTTATAACATTGATGAAAATCTAAAAACAATAAATCTTTTTGATAATGCTTTTGAGCGTATGGCTATTTCAAAAGATTTTATTGAAACAAGAACATATGATTGAGCTAAATTTTGCTACTTCACAGCAGTAAAAGTTACTAAAGATCATTTAGATAATCAAGATGCAAATCAAATTAATAATTTAATTTCACAAAATAATGCTGAAATTAATTCTATTAACAAAAGAGAAATAGAATTAGATAAAATTATTAAAGAATATGATCAAAAAATTAAAGCAACAACAGATTTAAATGAGAAAAAACGTCTTAATAAAGAAAGAGGACCTTTTGCTCTTGAAAGAGATAGAATCAGAAAAAAAAGAAATGAAATATCAGCTCACAATACGTATTTAAACAATAGACTTAAAGCTAATTTATTTGATGCCTTTAATAAAGCTAAAACAACTTTTGGACCTTCTGAACTTTTTGTTGGAGCAGACTTACATTTTGATATCACTAAAGCATTAAACAAAGTTCAAAGAATGAGATGAGAATTGTTTAATAAAGCTGTTGATGATCATATGAAACAAACTGAAAAAATTTTAAAATATCAAGCTCCAATTTCAAATTTCTTCAATGAATTTAATAGAGAATACTTAACCTTATTAAATCCAAATATTGTTGCTTTTGAAAAAATAGCTAATTCTAAAATTGCTTCAATTAAAACAGCGATTGGCGAGTTAATTAATGCAAGAATTAGAGAAATCTTAATTTCAGGTCGCGAATTTCCATTATATGATTCAAAAAACAATGGATTTGAATACAAAGATGCTGACAGTATTATTGTTGCTAAAAGTAGAAGCGAATTAATTGATATTTTAAGCAAGAAAAAACTTGTTAATGCTAATGATAGTGTTGAAAAAATCAACAAAGATATTTACTTTGTAAGATTAAGTCATCTTAAAAAAGATGGTACAAAACTTGTTGTTACATTAAGAAATATATTAACAGATGAAAAAGAAATGTTTGCAAATGGCTACAGTGACAAATACATCAAATTTAACATTGATGCCAGTGTAAATGACAAACGTCTAAGTACCCTAAACGCCTTATTTACAGCCGCAAACTACAAAAAACAAGTTACTCCAACTCTTATTAGAGAAGAAGGAAACATCCGTGATGAAAATGGCAAAAGTGTTAAAGGTTACAGTGTCTTTGTTGATGCTTACCAAGACATGACTGAAAAACTCTTACATGAAGTTCCTTATGCTGGAGAATGACTTGAAGGTGAACATTTAGTAAGCAAATTAAATGAAAAAGGTGAAATTGAATATACAGTTGAAAATGGTAGATATTTAGGTTTCACAAAAGATAGTCGTGTTAGTTTATGAGCTATTTTAAAAATGACTGACCCTAACTTCAAAGGTATTTCAACGGACTTCTTAAAATTTGTTGGAGCCCATGAATATGGTCACCATATTACTTTAAATGGAGCTCATGATTTAGGAAACAAAGGCAATAATCCTATTTATGTTTCAGCTTTAACTCCAGGTGCTACGCCTCATATTGACAACTACTATTCAAAAGATGCTCTTGAACTTTATCTTAAAGCTAGAACACACTTAGAATTAAATACAACTCGTTTGTTAAAAGAAAACAACGTTGTAATTGACTATGGTGAATATATTACTTTCGGTATTCCAAAAATTGAAATAGTTAATGGTAAACCAAACTTAATTTATAAAAATGAAAATGAAGAAAACATTTGAGGTACTAAATTAAATAAAGACGATATTATTGCAACACTTAAAAACAATAAACGTCGTTTCTTGCAAAACTTCAAAGGACTTCTTGAAGCGGTTATTGCTCGTCGTCAAGCAAATGGTTTAGATGACGATTATGAAAAATATCTAACTGCTTTTGATCTTTGGTTAATGAACTCACTTGACCATCATTCAGGTACAATTAATCCATCATTAAGAGGCGGCACAGCTAAATATATGGTTTGAGATAAAACTGAACAAAGATATGTCTTTAAACCAGGAAGTTTAGAAATACTTAAAGGTATTTTAAAAGATGGCAAAGGCAATATTGTTGAGTTTGAAGAAGTTAATGGTGAATTATTACCTAAAGTTGTTGAAGGCGTTAGAGATGCTAAAGGTAATTATGTAATAATAAACAAAGTTTTAATGTTTAATGAAGATGGTACACCAGTTATTAATGTTCCATTGCATGTTGACTTTAATGACAAAAGCTCACCTTATTATGATGCAAATGCTATTAAATATGTAAATAGTAAAATTAAAGCTATTATTGAAACAATTAAAAGTTTAATTGTTGAAAAATACAGCATTAATGGTTGGGATACAACAGCTACTGATTTAAGTCTTGAACCTAAGTTTATACTTTCTAATTTATATTTAAGTACTTTACTTGGCAAAAATGATGACTGAGTTGAATTAGTCAATAATTTAACTTATAAAGCTTTTGTTAATAATAGAAATAACAACACAGGTGAATTGATTGATGATTCAAAACCACTACCTTATTTTGATAATAGTGGTAAGCTAATTTATCCATCAAATAAAAAAATACCTCAAGCTTCAATTTATGCGAATATTAAAAAATATGCATTAGGAATTAAGCGTCCCGAAGATTTAACAAGTTTAATGTTTGCAATGAATACAGCAGGTGATTCATTTAATAATGTAGTTAATAGTGGTGAAGGTCACATTCTTTATGTAGATAAAGACCATCAATATTTACCAAATATTAATTTAGCTCAAACATTTGAAAATAACTTTTTATACAATTATGTTGCTGATATGGCGGACGTATTACAACTTAAAAAAATAATGAAATGATATTCTAAATTTACACCTGAGTTTATTGGAAATAATAAAAACAAACATTTATGATTTGCAAAAGACAAAAATGGTGAAACATTAAAAAAATTACCAGCTTTAGGTGAAATTCATGAAGTTTCAAAAATGTCAATTAATGAAAAAATTATTGAAAAAGATCCTGTCTTAAGCTTATTTAATGCCTTTTATTCAATTGAAGGTAGTCAAAAATTATTTGGTAAAGATGTTGCATTTACTGATTACAAAAAATTCCTTGAATTTGTCTCAGTAGACTTAAAACAAGCCAAATTGGACAAAAACACTAAAGTAGTAAATTGAAATATTGATTATGTAAAAGGCAAAGTTGACTTCAGTAAATTTATTGCTGGTTTAAGGCTTGCGATAGATGAAGATCAAACCCTTGAAGCAAAAGAAAAAGAGGCCTATACAAAACTTATTCATAATAATAATGAGCAAGAAATTGCAAATGAAATTATGTTTAGATTCTCGCATTCTAATTTAGGTATGTTAATGTCAAATATAACTATTCAAGATCTAAAAGATAATAATGATTATGGTTGAATATTTGATCAAAACAACGGTTATGGAGATTTAAGTGTTTCAACACTCAACACAATTAACCCTGACATTAATAAATTTGAAATATCACTTGAACAATACATTAAAACATATGAAGCAAAAGCTCAAGAATGAAGTGCTCAATTAAATCAATTTAACTTATTTGATGCCTTAATTTTTGATGGCAAAACATCATGTTATACTTCTCAAACAGTTGGAAATATGTTAGAAAGTAAAAACTCAGTTTATGGTCTTTTATTAACACTTGGTAAAGGTATATTCAAAAAAGCAAAACCTTCTCAAGATGTTCTTGATTACTACGGTTCAAAAACTGAACGTAAATTTAATGAATTTTTTACAGACTATACATATAACTTTGCAGAGGTTATTAACCGTGACAACTTACAAATTACTTATAGTCCATCAAATTCAAACTTTGGTAACATGCCTTCATATTTAAGTAATTTAAGTGAATCAAATACTGGTCTTGAATATGTTGTTGATGGGGCTGCAACTTCTAGATGATTAGACTTATTAATTAATTTTAGAGGTGACAAAAATGGTTCTGGTGTTAGAGATACAATTTTAAACTTTGAAAAAATGCTAAATGAAGAAGCAAGAAGAAGATCTCAAATTCTTAATACTAGATTTATTGAAAGAGTATTTGCAAATCAAGACAATATGCAATATGGCCAAAATTATAAAAATAATTACTTTGGACAATTTAAAACTATTAATAATGGTTGATTCAAAGACCGTTGATATCGTGATTTCTTAGACTTTAAACTTTATGATGATCAAGGTAAAGACATCAAAGATGATACTATCAGAATTAAAGATTTAACTGGAACAGTAGTTAAAACAAGAGCCAGAGCTTACTGACAATATTACATTCAATCTCAAGGTGTTGGAAGAAGAAATATCACCAGCATTTGAAGAGATTCAGATAAAGATGCAGTTGCAATGTTTGGTTACTTATCAACTGCAATAGCTGATAAAGCAAATTATTTAGCATTCAAAAATGTTAGAACAGGCGAAGTAAAAACGCTTCGCATTACAAAACAAAATGCAAGCAATATGTTCTATTACAAAGAACAACAAGTTAATAATGAAATTCGTCACCAAAATGGGGAAAAAGTACGTCACTACTTAATTGATGAACCATTTGATTTTACAGATAAAAATGGTCATCAAAAAGGTACTGGATTTGTTGCTTGAGTTTCAGATTATGCAATTATGTCTAGATATAGAAATAGATTACTTGATACAAATGAAGAATATTATGTATATTTTGCTTCAGATGAACAAGGAAATAAAGCCTTAGAAGCAGACTTAGGAACCTGAGATTCAATCTCAGAAAATGGCAAAACTTTCTCACAAGCACCTATATCAATTTATAGAGATGCTGATGGTAACTTTAGACTTAGAGTGCAAGATCAATTTAATGGGGTTATTTAACCAAAATAACTATAAAACAGGAGGTTTAGAAAATGAAAAGAAATAAATTAATATTATCTTTAGCTAGTTTAAGTACTATTAGTACTGCCAGCATAGTAGCTGTTTCATGTGGTAATAAAGCATATAAAACAAACTATGATCTTGGTCTAGTAACTGAACCTATTAACTCATTAAATTACTTGAAATTTAACTCAGTTTCAAAAGTTTTACCTTCTATTGTAGAAAGTCCATTAAAAACAGGTCCTAATGAAGCTCTTAAAAGAATTTTAAGTTTGCCAGAAATTCCTATGGGAGTTTATGGTAATGATGACAAAAGTAATTCTATGGATGATTACATAGCAAATAATCGTGCTCCAAAAGAAGCATCTGGTCGTTTTTATCCTCTAGATCAATTTGGTTCTGCGCCAGGAACAATTAACACTGACCGTTCTGAGTATCAACCTGTTAGTGTTGTTATGACTAAAAATAATAAAATCCTTTCACTTCATATAACTTTAAATAGAGGCGAAAGTAGATGAAGTAATAATGATATAGTAACTGCTGATGATTACATTGATGCAATGCATTACATTCTTGATATTAATACTGGTAGTCAAAAACAAACAAACATTTTGCAAAGAAAATTTAGATCAAGCAGTAGCCTTATTGATGCTCAACAAGAGTACATTAAAAAACACAAAAAGGCTTATTCAAATCCATTTGCTTATCCAAAATTAATTAAAGAAAATGGACAATGAATTTATGATGTTTTAAATCCTAATTACAAACCATGAGCTTGTCAATTAGAAAATGAGGCTGATAAAGCTGAAGTTGAAAAAATTAAAGAAGAAGCTCTAAAATTAGGCCTTTATTCGGGACGTATGTATTGAAATTATGATAATCAAACAATCTTAGCAGCCATTCCTTATTCACCAGACTTTAACGAAAATGATGAAATTACAACGGTGATGTTACCTAATCCAGAATATTCATTAAGTCGTCACACAGCTGAAGAATTAAAATTAATTCCTCAAAGAATTGCAACAAAAATTAGAAAATATCTTTATTTTGATCCTCGTCAAAGTTATTCTGAAACAAAATTTAAGCCTTTAGTTAAAAAAGCTAAAAAGCTTAAAAGCAGAATGAACAAAAATGTATCTTTTGAAAAAGATATTAATGAATACAACCAAGAAGTTAACAAACTTTATGGCAAAGAAAATACTTTAAATAATAACTCAATTGACTCAAGAGAATTTATGGAAAATAGGGTACTTGCTCTCGATGAATTTTCACTAAGAGTTGAATATGATAGCAATGAACCTACTTCTCTTTCAAATGCCTACAGCGATATTCAATCAACATTAATTCCAGTTAATAGAAAATTTGTTGAAAGTATTGGTGGCATTAGAGAATTCGGTTTAGATAAATCTAAATTTTTAACTAATGGCCCTTTCTATATTAAAAATATAGTTTTAGGTCCTCAAGGTTATATGGAATTAGTTAAAAATAATACTTATTATTCATCTACTAAAACAATTAGTGATTCAATTAAAATCTACTTTAGCTCAGATGCAAATATTAATTCAGCTATGTATGATGATGGTTATATAGCTGCAACTAAAATTCCAGCTGTTCAACAAATTAATTATTGAACCAACAAAAGTTATCGTCCATTTATGAAAAAATCTTCAGGTTTTGGTACTATAGCCCTTGCCTTTAATTTGGATCAAGAAACCAATAAAAATTCTTTAATTAATGATGTTGATTTACGTAATGCCCTATATTTTGGAATTAATCGGAACGAAATGCTAAACATAGTTGGTTGAAACTCATCATTCCCAGTTATTACTTGAACTGCTTTTGGTCAAGGATCATCAAGCTTTGGTGATGCTGTTGAAATTGGTTTTGATCATGACTTTATGAAAACAAAAGTTTCTGACAAAAAAATTCCAATTCAAAACTATAATCACGTTGACCACTTAGCAAAACAATACAATAATGAACATGTTGATAGAACTGATTTAGGTTATAACTTAGAAATAGCTAGAGCTTACATGCAAAGATTCAAAGATAAACATCCAGATGTAAAACAAGTAACTCTTAAATATATTTCTAACTCAACTGATGAACAACAAAATGCAGGTATTGCACTTAAAGACTTTATCAAAAAAGCTTTTGGTGACTACTTAATTATTGATGTTCAAGGTTTACCTGAAAACGTTTATGAAGACTTTAGAACTACAGGTAAATATGATCTTATTTATAGAAACTTTGATACTTTTGGCTCAGATAGTTATAGTTATGTTAAAGTCTTCTTCAAACCAGATGAAATTGATAGAAAGAATCAAAAAACCACTGGTTTTAGAAACAACCCTTCTGGTAGTTGAACTTACCAAAACTACTTTGAAAGCTTAGGTTATGTTTGAGATGATAAAACTCAAAAATTAATTTCAAATAATGCAGCTTTAGTTGATGAAACAATTAAAAGACTAAATATGGAAACAAAACAATGAAACAAAATTCTTGATTTAGCATTCAGAAAAACTTATGTTGATCAAAAAGATGCTAAACATGAAACAATTACTAAATTTACTGAAAGATATTTAAGATTCTTTTCAAACCAATTCAATGAACAAGAAAAAGCAGAAGGTTGAACTGAACAAAGTGCTTTTGGTATTATTACTGCTTTAGAAAAAATTATTCGTGATGCAGCTCCGGTTGTACCATTAATGGAAGTTGATACTTACTGAGAAATTTCAAGAGTTAATGGAACAGAAAGCTTATTCACCTACTCATTACAATTTGCTTATGACGTGGCAAAACCTCCAAGAGCAACCTTGCCTACAGTCATAAAATCTTAGAAATGAGACAAAATGCAAAAAAGAAATGATCAAAATTATTTCACTGGCACAGTAAATAAAGATCAATATAATCTTTATAACCGTGAAGTTGATTTTAGCGGTAAAGCTAAAAAAGATAATAGATGATGATCAAGAATTTTTAACATTCAATCTCCTGCTCTAAAAGTGTTATTCAAAATTTCATGAATGATTTTAGAGTTCTTACTAATTGCTTGAGTAGTTATAACAATTACATTCTTCTTAATTAACTCTGTGCCTGGTTCAACTACTATTTCAGCTGGACTAGATGATGCAGCTCGGCAAGCAGCTGAAGCTCAATATGGACTTAACTTGCCGCTTTATCAAAGATATTGAATTTATTTAGGAAACTTACTTCATGGAGACTTTGGAGTTTCACTCTCAGTTTTCCCTGGTGAAAAAATTAATTCATTTGTCTGAGTTAGATTCCTTAAAAGTTTCATGATAGGTATTTTCTCAGTATTCTTAACATTAATTATAGGAATTCCTGTTGGTGTTTATGTTGGTATGAATCCTGATAAATTACCAGACCATATTGCAACATTAATTGTTTCAGTATTCTCATCAATTCCATCATTAGTTTTTGCCCTATTGCTCTTATTAATAGGCCGAAGCTTAAATATGCCTTACTTGTTTAATGAGCTTGATATTGCTACATATGTATTACCAGGATTAGCCTTAAGTTTAGGTTCAATTATTGTTTATATTAAATATATTCGTACAGAATTAAATCGAGAATTAAATTCTCAACATGCTAAATTCTGCTACTTAAAAGGTTTAAGCAAACGTCGTTTTGTTTGAACTCATGCTCTTAAACCTTCATTATTCCCTATTGCTACATTCTTCCCTGTTGTCATCTTTGGTAGCTTTATTGGATCAATGTTTGTTGAGCAAATCTTCTTTATTACAGGTAGTGGTGGATTATTAATCCAAGCTATTACTTCAAAAGACTACAACATTATTCTCTTTATGGTTACATTATTCTCATTAATTACAATTCTTTCATACGCATGTCGTGATGCTCTTTACCAAATCATTGATCCTCGTGTGAGAAGAAGATAGAAAGGAAAAACTATGAATTGATTTAAAACTTGAGTTGAAAAGAAAAAACGTCGACAAGAAAGTCCAAACAATATTCATGGTAAGGACTTAGCTCCAAATCCTTTCTTACAACCATTAAACTATAAAAAATGAGAAATTATCGGTAACCTACTTGAATTCCATGAAACCTCTTCAATGCATAAACAAAAGAAACCATTTTTAGAATTCTTCTATCGTTTCAGTCGTTCAACTGCTGGAGTATTTGGTTTTGTGGCTCTTGCTTTATTAATTATTCTCGCAATATTCTTGCCTTTTACAACCAAAGATCCTAATACTGTTAATACTGAAAAAAGATATTTAATCTTCTTTACAGATGGACACATTTTTGGAACTGATGCTTTAGGCCGCGATATTTGAGCAAGACTGTGATGAGGTTTAAGATATTCATTAGCTTTAGCTTTTGTAGCAACATTTATCCAAGTGTTTGTCGGCTTAGCTATTGGTATAATGATGGGACATTTTAGAGTCTTTGATAAAATAATGACTTTCATTATTAAAATTATTTCAAATGTGCCTTCTATTATTATTCTTATTGTTATTACTATTATTCTTAAACCTACATTTTGAGTTATTGTCTTTGCTTTAACATTTACTTCCTGAACTGGAATTGCTAACCAAATGCGTTCACAAGTTATGCGTGCTAAAAACTTTGAATGAGTTAGTGCTTCAAGAATTCTTGGAACTCCAACCTACAAAATTTTACTTAACTACTTACCAGTAGTTGTACCACTTCTTGTTACTGAAATAGTGTTCCATATTCCAGGTGTTATTCTTTCAGAAACTTCTTTAGCCTTCATTGGTTTATCAATAACAGATATTCCAACATTAGGCAACTTAATCAATGATGGTTCAAAAATCTTTACTACTTTCCCTCGTTATGTTTTAATTCCTTCAACAATGTTAATCATTGTAACAACAAGTATTCAATTAATGTCAGCTGCAATTCAAGACAGCTTACTAAGACAAAGATAATATGGAAACAAAAGAAAAGAACAGAGGGTATTCAAAAATATTTAGAAAGCCTATAGTTTATCAAAACAAACCAGCGCCGCTTGACTTGTTTGAAGTAAAAGGCAATCCTAAAAACAAATTTTGAAGAAGTTTTAAAGATGCTTGAATTAACTTCGGAAGAAATATAGCTAAAGGTACAAAAAAAATTGGCGAATGATTACATGTTGTTAAAAAGCAAGAAAAAAATAAATTAGATTTTAATCAATACATTGAGAACGCACAAGTTGAAGATGTATTCGGAACACCTACAAAAGTGGCTGCTGAAATTGAAGATGTTTACTTAACATTTAGAAATCCAGCAGATCCTAAAGAAAAGAATCTTGTTTTAAGAGGACCTTCTTTAAAAATTTATGAAGGAAAAGTTCACGCCATCATTGGTGAAAGTGGTAGTGGTAAAAGTGTTATTACTTCACTTCTATATGGACTTACAGGTTCAAATGCCATTATTGAATCTGGAAAAATTAAACTTTATGGCTTAGAAGTTCAAGATTACACAATGTACAATTGGGAAAAATCTAAACTTAGAGGCCGTGTTGTTAGTGCAGTATTCCAAAACCCTATGTCAATTTTGGACCCAACTATGAAAGTTGGAAAACAAATTATAGAAGGAATGCTAATTAATAAAATAGTTAAAACTAAAAAAGAAGCCAAAGCAGAAGCTATTAAATATTTGGAAATGACTAAAATTAATAACCCACAAAAAGTTATGGATTTATATCCTCATGAACTTTCGGGTGGTATGATCCAAAGAATTGCAATTGCAGCTATTGTTTCATTGAAACCTAAAATTTTAGTCATGGATGAGCCCACAACAGCTTTAGACCCAACAGTCCAAGCTTTAGTTTTAGATATTATTAGGGAACTTCAAGAAAGATTCCATATTGCTATAGCATTCATTACTCACGACTTAGGTGTTGTTGCTTCAATTTCTGATTTTATCAACATCATGTATGCTGGTCAAATTATTGAAAGTGGAACAAAAGAAGAAATATTACAAAATCCACAACACCCTTATACTTGAGGCTTAATTGTTTCAATGCCTGACTTTAATAAAGGTACAAAACTTCAAGTTATTAGAGGTGCTGTTCCTTCAAACTTAAATAATATTGATGGTGATGCTTTTGCAGTTAGAAATGACTATGCTTTAGGAATAGATTTCGAAGAAGAATCACCTTTCTATCAAATCTCTCCTACACACTTTGTTAAAAGTAATTTGCTTAATGGACAAGCCCCAAAATATGAACCTCCAAAAATTATTCAAAACCTTTGAAATAAATACAATTCAAAATTAGAATCTATTTATGGTACTGACAAATACGTTGATCAAAAAATATATGATAATTTTGTCCAAAAAGCCTATGAAAATAATGAATTGGTAAAAATTTTAAATACTAAAAGAGATGCTGAATTAGCTTCGAGAGAAGAAAATAACCAAACAAAATCAGGAGCAAATAATGCCTAAATATTACTACGAAAAAAGAAAATTTGGCAAACCACTTGCTAGAGAAATTAGACAAGGTACAGATGAAATGCTTAATGCATATCCAAATAAAAAAGCTTTAGTAGAAATAAGAAATTTAGATATTGATTATGGTTTTGGAGCTAAAAAATTTAGTGCTATAAAAGATTTAAATTTGAATATTTATGATGGCGAAGTTTTAGGACTTGTTGGTGAAAGTGGCAGTGGTAAAAGTACCACTGGTAGAGCAATTATTGGTCTAACACCACACACTTATGGATATATAAAAATCCTTGATAGAATCATTCCAAAAAACTTGGATAAAGGTTTCAAGTGAGGTAAAAAATATAAAGAAATAATTAACTTTATGGTTAATAAAGTACAAATGATTTTCCAAGATCCAACTAATTCACTTAACCCTTACAAAAATGTTGAAACAGTTGTTGGTGAAGGTTTAACAAACTTAAAGAACTCAAAATATATTTTCTTAACACGCTTTGATCAAATAGTTTTAAAAGAAGTAAATAGATTGATAAATAAAATTGATTCAACTAATTCTTTGATTTCTAATTTATTAGAAAAATGTCGTGAAATAGAAGTTTCAATTGAAAAATCTTATAACTTTGTTTTTAAAGAATTAATTGATGAAATTAATAGAAGATATACAACTAATGCAAATTACAAAGATGCTTTAGAATACTTAGAGTCTCAAAAAGTTGAAAGAGATTCATTAGATAATTTAAGTGAAAAAGAATGTAAAAAACGTTTAATCATTGACATTCTTAAACAAGTAGGTTTAGATGAAACTGTACTTGGTCGTTTCCCGCTTGAATTCTCAGGTGGTCAACAACAACGTTTAGGAATTTGTCGTTCAGTGGTTTTACAACCTAAATTATTAATCGCAGATGAGCCTATTTCAGCTCTTGACGTTTCTATTCAAGCTCAAGTTATTAACATTTTTAATGAGCTTAAAGAAAAATATAACTTAACAATTCTCTTTATTGCCCATGACCTTCGGATGGTTGAATATATTTCAGATAGAATAGCTGTTATTAATAAAGGTGTCTTACTTGAAGTAGGTCCAACTGATGAAATTATTCATAATGCTCATCATCCTTACACAAGAAGTTTATTAGATGCCGTTCCTTCTATTGAAAGTGAAAAAGGCTCATTATTAGGTTATATTTATGATCCAAGTATGCATAAATATGATGAAAATAATCAACCAGTTTGACAAGATTTAGGAAATAATCACTTTGTGTTAGCTACCGATGAAGAATTAGCTAAATACAAAAGAGATAGAACTAGACTTAATGAAATTGAAGCTAAAAGAGAAAGAAATGAAAAGATTACACTTGATGACTTTATCATTGAAGAAAATGTTATTAATGAACAAGAAAAATATGATCTTGTAACTAAAATTAAAGAAGCTCAAATTGACTTAAATAACTATAATGAAGGGTCAGAAGTAAAAATTACTCCTTCTTATGTTAAAACTGCTACTGTTATTCAAGAAGAAGTTTCAACTTATGATTCACTAAAAGAAACTACTAAATTAATTAAAGAAGATGACTTACCTGAAGTCAAAGTAACTCCTTCTTATATAGAAGTGGCTAAAGTTATTGAAGATGATGAAATTAAAAAAACCGAGAAATCTTCAACTAAAAAAGTTGAAAAAATTACAATTATTTCAAATGTTCACAAAGATTCAACTGTAGAATTAATTCCTGAAAATGATAAAATTACAGTAGAAGAAAAACCAAAAAAAGTTAGAAAAACTAGAAAGGTGAGTGCAACATTGAAAAAACCTACTAAAAAATCAACAACCAAAAAACAAACAAAAACATCTACAATTACTGTTGACTTATCAAAGAAAATCAAACCTAACAAAGGTGTTACAAAAGCTGAATTAAGAGCACAAGCAAGAAAAGAAAAATCTGCAAACAAAAAAATAGTAGAAAAACTTGCTAAAGAAAATGAATAAAAAATCGCAAGCAACTGCGATTTTTTAAACCTAAATTTAAACCATATTTTTAGTTTTTAAAACATAAGTTTTGAAAAGTGTTTTGTCTTCTGAAACATATTTAAATTTAAGTTCTAAATGATTTTTGCTTACAAATTGATAATCAAAAGAAATTTTACTTTGATAATCTGCGTTTAGTTTTTGAAAAACATCTTTCACTATTAAAGGCACAACATTTTCATCAATATATTCTTCGCCTTTTTCACCTATTCTAATGTACTTGTAATAGTTGTTATATTCTAATTTAGGAAATTCTTCAAAAGAATTTATTGTTTCTTGTTTTTCAATTTCCTTATGTTTACTTTTAGCTGCAGATACAACAACAATTGGAGTAACAACAGCTAAAACACCAACAGTACTTAAGAGTGGAATTAAAATTTTCTTTTTCTTAGCCTTATCCATTTTTTACCTTATATCTGTCTAAAGAATAAATTGAAGAAAAGTCTTTTAATTTTAAATATTCTTCAATATCTTCTCCTTTAAATTCATATGTTGAATTTGAATCAAAAATATCATTATTTTCTTCGTATTTTAATTGAATTGTTCCTTCAACATGATCTAATATTTTTCTCTTAAATAATTGGTGATTGTTGATATTTAAAATAATAGGTGAAGAAGTATTTGAATATGCTTTATCATTGAAATAAAGAACATAATTTGTTTTTAAGTTACCTTTGTAATTATATGGAATAAATAGACCTGCTTTTTGACTTTTTGTTGAATTTACTAAATAACCATTGTCATAATCATAATCTATGAAATTTTCGTATGTGCCTGTTTTATAGTCTTTTCACTTAGTTGAATCGTCACTACTTGTTGTGGTTTTAGTGTAGATTTCATTCTTAACTTTTGTAAATAATTCATTTGCAATATTCTTGAAGCCATAATTAGTTAAATTTTGAGAAGCATAAAAACTTTTGCTAATCTTTAAAAGATTTAATAACCTTGGGAATTGTTCATAATATTCTTCTCATGTAATTCCATGTCAAGGTGCAGTATAACCATAAGGTCAGTGATCTGATAAACTACTTTCTTTTGCTTCTTCAGTCTCAATAGTTGCTAATTCATTTTTTCTTTCATATCTATCAAATTCATTAAGTTTAACTATTGAGTTTAAATAATGAATTTTAAGTTCATCTTTTTCTATGTGACTATTGTAACCATTGAAATTAAATTCATATAGTTGAGGTATTTTGAAATATACCTTATTGTCTTTAGCATTGAAAGTATATTCTCTTCTTTCATCATTGATTTTCTTGATTTCTCAATATTGTTTTTCTGTTTTTTCTTTACTTCTAAAGTAAACTCTTAAAATACGGAATTTTTCGTAAGGTAAATTTATAATTTTAGGTGAAAGGATTGTTAATCCATATGCACTTGTTTCTTCAGTAAATTTCGAATACCATTTTTTAAAACTAAGTCTTTCATTTAGTAAACTGCTATCCATTGTTTCCAATTTTGTGCAGCTTCTAATTATGTTTCCATCAATTCTTTTTCCATTTATTTCAATATCAAAACCTTCAAAGAATTTTTTATGTAAATTTTTGGGTTGAATAAAAAATATTTTAATTGCAAATCTATCTTCTGTAAAATATCTTTTTATTTCAATATTACCATTTACAATATTGCATGCATCTTTAAAATTTACATCATAGTGATCTTTAGTTAAATAAGATGGAGCATCTAAATATTCTGATTTGTATACATAACGTTCTTGCGAAATATCATGCACCAATTGTTGTGGTGGTTGAGGTGGATTATTCGTTAAAATAATGGTACAAAAACTCCTCTACTGCTTGTTTTTTATACTTATAATAAGTATTTTTGGAATAATAATCTTTGAATCAATTTTTAGATTGACTTTTTAAGAAATCCTCTTTGATAATTTGCGCAAGTTCAGGTTTCATAATAGATAAAATATGCTGACTTTTATTTTTTGGTTTAAAGATGTTTTTAAGTATGTTTGATTTGTTGCCTCCTCCAATTGTCTTTTTGTAAGCTTGCTCATTGACTTTTTCCATTTTAAAGATATTGTGTAAAACACTATTTTGATCTTTATGTGAAATTAAGCCCATAAAATAATCATTAAAAGTTAGATTCATTTTCCTCCTTTCAATTTATATCTTTTCAATATAAATAAAGAAGAGTCAAAAGCGGAAAAAAATAGGAAAAAAGCATGATTTTTCATAAATTTTTCCTATTTTTTAATTTATTTAATAATTTTGTACACAATTTAAAGAAGTTGTACGCCTTCTTTAGCTAAATCATCAATTAATTTTTTAGGTCAAATACCTACTTGAACTTCACCAATGTGTTTCTTCTCTAACAAGAACATTGAAAGTCTACTTTGCCCTATACCACCGCCAATAGTTAAAGGGAAAGTATTATTAATAATATTTTTATGATAGTCTTTAAATTCATCTTGAGAAACATGACTAAATTCAGCTTGTTTTAATAAGGAATCTTTATCAACTCTAATTCCCATTGATGAGATTTCTAAAGCTCTTTTATTAACTGCATCATAAACAATTAAGTCACCATTTAGTTTTCAGTCATCGTAGTCAAAAGCACGTTTTGATTGAGGCTTATTATCTGCTAAAGGATAACCAATTTCATAAATAAATATAGCCTTGTGTTTTTTAGCAAATTTTGTTTCTCTTTCTTCAGGGCTTAATTTTGGATATAAGTTGTAAAGCTCACTACTACTAATAAAAGTTACATTTTCAGGTAATTTTTGACTTAGTTGTGGAAATTCAACTAAAAGTTTGTATTCAACATGACGAATACATTTAAAAATTGTGTTGACTATTTTTTTCAAAAATTCAAGATTACAGTCTTCTTTTTTAATAATTAATTCTCAGTCTCATTGATCAACATAAAAAGAGTGTAGGTAATCTAAGTCTTCTTCTTTTCTAATTGCATTCATATCAGTTCAAATGCCTTCATAAGTACTGAAGTTGTATTTCTTTAATGCATATCTTTTTCACTTAGCAAGAGAGTGAACTATTTCAACAGTTTCATCAAAATTTTTAGGAACAAAAGATACAGGTTCTTCCCCATTTAAGCCATCATTTATTTTGGTATTTCTAGCAACAAATAAAGGGGCTGAAACTCTAGTTAAATGTAATTCCTTGTTTAATTTTTTAGTAAATGCAAACTTCAAATCTTGAATTGCTAATTGAGTTTCTTTAATGTTTAATTTACTCTTGTACATTTTCGTTAGCCTCTGTTTTATAGTTGTTTTGGTCATTTTGTGGAATTGAATTTTCTTGATTATTTTTAGCTTCTTGCATAGCTTTTTTTGTTGTTTTCAAGATGTGTAACATCATTGATAATTGTGATATGTAAATTAACAATGACAATGTTTGTCATAATAGTGTTGAAGTTAAGGCTGGAGTTAATTTTCCTTCTGCAATAATGAGTGATACTCAGTATGCAATTCAAAAAATATTTGTTAATACAAACATTAAAATTAAACCAACTGATAAGCCTTCAATATCTCTCTTTTCAAATGTTTTTAATACTTGTGGTAAGAATGCAAAAGTTGATATAACTGGAATAATTTGAGACAAAATAGCAGTTGTATTAGGATCAAAATTAAGACCATTGCCTTCACTGTTTTTGTGATATAAACCGACAAAACCAACAATTGCACAAATTGTAGTTAAAGTTAATGTTATTCCTAAAACTACAAATTTTCTTTTGTTTCTTTTTACTTTTTCATCATATTTGTACATAAAGAATAAAAGTACACAATAGACATAAGCACACAAAATATTGGCATAAACTACAGCCGCCATTTTAGCTTCTTCCCCTGTGAATGATCCAAATATTATTCAGCTACATAATGCAGCAAAAAATATTCAGAATGAATAGAATTTAACCTCCCCAGTTTTCTTTCTTTTTAATAAATAAACTAATTGAGGAATTGATACACTTATTGTTGCAATACATGCTATCATTCCTACTATTGCTACAAACGCTGCTATGCCATCATTAACATCTTTATTTATAGCAAAAGAACTACGTATAAAATCCATGTTTTAATTATATATTAATAATCTTTATAAAATAATAATTGAAATATTAAAAATGTTAATTCCAAAAGTGAGATCTCTAAAACCAAGGGTCTCTATTTTGGAATTAACACTATTTTTTACAATAATACTTTATTTTATTTTTTAAGTTTTTATTATTTTTCTAAAAAATAATCATAAATTTATTTGGTAATTAAATCGCATAATTTTCAGTTTTATCTACTTTTATTTTAAAATTAAACTATGAAAAAATATTACATTGCCTATGGATCTAATTTAAATTCGGGACATATGGAAAACTTATGTCCCTCAGCAAAATTTTTAGGTAAAGTTATTTTAAATAATTTTTCTTTAAGTTTTGAGGGAGAAAAAGATCGTTGTTATTTAAATATCAGAAAAGTAAAAGACAAAAATATTGAAGTTGGAGTTTTTGAATTAAATGATAAAGATATTTTTAAATTAGATGAATATGAAGATTATCCTTTTTTATATAAAAAAGAAGATATTGAAATTGAATTAAATAGCAAAAAAATTAAAGCTTTTTATTATTATATGGATTGCGGATATAGTCCTTGCAAACCAAATAAAGAATATGTTGAAATGTGCTTAAAAGGATACCAAGACTGCAGTATTAAAAAAGAACAACTTAGAGAGATTTTATACAAGTAATATACAAGTAATATACAAGTTGTGTTATAATCTTTTTATGATTAACAAGGAATATAAGCCTATATTCAAGGTTACAAACAAAATAATTGAATTAATTGGCAAGATTAAAGAGAAATTGTCAAGCGTTTTTTTATGCAAAGATTTTTTGATTTTGAATAGAAAAATAAAAATTAAATCTATTTATTCAAGTGTGAAAATTGAAGGTAATCCCTATGATTTTCAAACTTTTTATAAAGTCATTAATAATGAAGAAAAAGAACAAAAAGATAAATATAAATTGGAATTTAAAAAGGTGCTGTAAACTAATTGTGGGAATGAATTTAAATGAGAGAACAAGCATAAAGCTTGTTTTTTTCTTCTTTCTATCAAACTAAATATAGAAAGGAGGTGAAATTATGTCTTATACAGGAAATTTTGATACTTTTTCAACATTCTTTCAAAACTATGGATTAAATTATGACAAGTTAGAATCAGAAAAAAAACAAATAATAATTAATAAAAAATTATTACCAGAGAAAACGGCAAATTCTAAAATTTCTGAAACTAAAACTGAATCTTTGTTCAAAAAGATTAGTTCAAAATCTTTATTTGAAAAAATGTGAAAGAGTAAAAAGTATTTAAATTTTGCTGATAAGGCATTGATTTTTTTATCTACTCTATTCAAAATGAAAATAACAATATCAAAAAGAATTTTTGATGAATTAGTATCAATGAATTCAAAGGTATGTTTTTATGTTTATATTTTTTTGATATATTCAGGAGCTAGAAGAAAATTTGTAGAATTAACAGGATTAATGAAATATGGTTTTGCTAGAAGTTCATTATTTTTTGCATACAAAAAATTGCTTAAAAGTAAATTGATTAAAACCATCAACAAAAACGATTCTTGATATACAAATTTTACTTTTGTAAAATTAACTTATAAAAATTGAAAAACTAAAAAAGAACAATATTATTACTTAAAAAATGAAGAGGTTTGAAGATTATCTTTAATCTCATCATTGAAATTTATACCTAAATTTTTGTTTTTAACATATAAGTCTAAAAAAGTGTCTAAAAACAAATATGAAGTAAATACTGATAATAAAGTTTTAATTAATAATAAGTTTTTAAATTTGTCAAAAAATCAAGGCTATGTCTTTTTTAAATCAGTTGTTGCTGTTCTTGGAATTGATGTAATTAGCTCAGATTTACAAGCTTTCGAATATGTTAGAGAATATGACCTTAATTCACATTCTTTTAAATGCTTTCGTTGGCTAGTTATGAGAATATAACTTATTACAAAAATGACTTTGGAATGTAAGGCAGTTGTTTATCAAAATGACTGAAAATTATATAAAAAGTAGGTTATACAAAAATTCACTGATATTTTAAATATCTGTTTTTTAATGTTGCATTTTGAAAAAAACACTTGACATTATTTAACTTTTGATATTTGTCTATTCAAAATTGAAGGAATAAGTTATTAAGACTTTGGAATGTAATGGAAAAGTCTTAATATATGAATCATTGATTCATAAAGTAATCTATTTAACTATGAAAAAGTTTAAATTATCACAAATTACGAGAATTTATTTAAAAATTTGCAAAAAATCTAACAAATCTTGATCAGAAATTAAGAATTTTAAGAATATTTATGATTTTGTGTTTGCTAAATCATCAGAAAAATATTTTTTTATCAATTTAAAACATATAAATGATTCAAAATTTAAGCAAGATTTATGGAATTTTTCGAATTCTTACAGAGTTTATGCTTGTTATCAGATTTATGAAGAAATAAAAAGCATCAGGAGACCTCTTGAACAAGTATAAATCAATTTCTCTCTGCTCTCTCATTAGCCAAGCAGCTCGGCAAGGTAATCAAAAATGGACCGAGCAAGCAAGCATGGAGCAAAATCCCAAACTAAAGTGGAATTTTTAAGCTAAATTGTCAGAGAACTACCTTCAAAAACTCTCTAAAAAATAACCTAAAAAACCACTTGATAATCAAAATGGTGCTTGTTGTTTAGTTTTAGTAGCAATAACAGGTACTATGATGGGAACCTATACAGCAACGCCGATAGTTTATGCTAGTTATCAGATTTATAAAAAATAAAACAATAGGAGAAAAATATGGAATATAGTCAATTTGAAACAGAACTTAAAAGAGCCTTAACAAATGATGCCACATCAAAACTTATAAAAGAAATAATCAATAAACCTTTTAATTTTGTTTCACCAACCAACCCATTTGATGTTAAAACTAAAATTAAACAATCATTTTTGAAGCATCAAGAAAGAATTTATGAAAAATTTATTCAATATTGTGCTAAACGTTTAATTACTCAATATGGTTTTAGTTTACAAAGAAGTAATTTTAGATACAATTTGCCTCTTAAAGAGGGACAACACTTAGGAGACATTCCTGAACAAGTAATAATCAATGCATCTTTCATTTTTAGATTGAATAATAATTCAACAAAAGATGAACCAACAGATATGTTTGTTGTTTATTTAAGAAAGAGAGATTGCTATTCACATAAAGCAATGAAAAAAACTTGTGAGAATATCATTAAACAATTAGAAGCCTTAAAAATTATTTACCCAAAAATGAATATTAAGGGTGCTTTATGATTTTTAGATAATGATTATATAAAGAATATTAGATTATTTAATAATTTTTATTATGAACAAGTAGACTTAAGACCTTATTATGGAGCTGATTTCTTTGAAATATTAGATAAAAAAGAAGATTGATTAAAGATAGAAAGTTTTATAAAAAAATTTAAAGAAAACAATCAAAATTATTTTTCGAAAATGCCAGATTTAGATCAATCAGATGAAGCACTTAGAGCATTGCTTTATTTGGAAAAGTCAGAATGAACCAAATTAAATTCAAATAAAGAAATTTATAGAAATATTAAAGAAAATATTTTCAATTTAAATGATAAAAATTCTAATTTATTCAAAGCAATTGCAAAAAGGAACAAATCTTATTAATTATGAAAGAATTAATAATTTATTTAAGTTGAAAATATGAAGGAAATATTTGAAAAATTTATGAATTTTTAAAAACTTACAAAACAGTTGCTCAAGCTAAAATAGATGAAGTATTAAATGAACTAAAACAAAAAGGCATTAAGTATATAACAGCTTTTGATGATAATTTTCCAGAAATATTAAAAAATTATCGCTATTCACCATATGTTCTTTTTTATAAAGGCAATATTAAGATTTTGAAGTTACTGGAAACTCAAAACAAAAATGAAGTTTTCATAGAATGAGATTTTGAAAATAAAAAAATTTTATTCACATCATTAAATAGTAAAGGTAAATCTAAAATCATTTACATACTAGATTGTGGATTTAATCATCTTAACAAAAAAATTAATTATAAAAAAGAGTTGTATATCACACAATTTCCATTTCAAACATTGCCTAGCAAAATTAATCAAGAATCAAGTGAAAAATTATTAAATTGTTTATTTACTGCATAAGTACAAAGGAGAAAATATGACTAAAAAAGAATACAAAGAAATTAATAATATAAAATCTAGAACTTTAAAAATGGAATTAGTAATAGATTGATTACTAAAAGCTGGTGTATTCAATAGAAGAGAAACTTATTTACTAGATACCAAAGATTTTAAAGTTGAAAAATTAATTAATGAATCTATTAAAAGAAAAACAAATTATAAAAACTGACAAGAATTATTTAAAAAAGAGCTTGTTAGATAAATATGGAATCAATTAGCAAATTAATAGATTCAAAAACAATAATCAATTTTGACATTGCAAAGTTGCAAAGTATTAATAAATATTTTGGATTTGGTATATCTGATATTAATTTATTAAAAATCAATCAGGTTTTATTTGATTATGAATTATTAAAAACTCTTAGATTAGAAAATAATTTACTTCAACTAAATATATATTATGAATTACTAAAAGCATTTAAATTGAATAAAAGTAATGATTTTAAAAAGAATTTCAAATTCCAAAGAATTAAAAATGAATTAATGGAAAAAATTAAAAAAATTTTTAAATTACTTGGAAATAATCAGATTGAAGAAAAAGAATATCAGGATTACATTTATGAAAAATATTGTTATGGCTTCAAAAAATCTCCTGAAAATATTTCATTCAAAATACAAAGAATTTTTATAAATATATTGTCTGAATATGAAAACTACTTAGATAGTTTTGATTAGAAAGGAAAAAAATATGGTTTTATATAGAAAAGGAAAAATAGTTTACAAAGATGGAAAAAATCTAATTTTTGAATCAGATAATGTTGGATACTATGTTACGTTTCCAGATAACAAAAGGATTAAATTAAATCAAGAATTAAAAATGTGTTTATATGAAATTAAAAATGATTTTACTTGATCTTTATATGGTTTTAAAGAATTAGAAGAAAGAAAATTATTTGCTGATTTACTTAATTTAAATGGCATTGGTCCTAGAGTAGCATTTAATGTTTTAAATGTTGGATTTGATAAAGCTATAAATTTAATTAGAGATGGCAACATTGAAGAGATTGCAAAAATTGAATATATTAATCCAAGAATTGCAAGAATAATTGTTGAAGAATTAAAAGAAAAATTATCATCAAAATCAAAAGACGTTAAAAAGTTAAAACTATGTTTGAAAATTATGTAACAACATATAAATCATCAAAAGAAATCAATGAAACTTTGAAAATGTTAGGTTTTAAAGACTCTCTTATTGATGATGTAGTAAGAAAAATCAATTTAAATCAAGACATAGAATTTTCACTAGAAGAAGCAATTAAATTAATGTCTGATAAACCAAAAACACTATAAAACAGTCATTTTAGTAAAATGTATAATTTAACACCAATAAATTTTAAAGAGTTTAGAGGACAAAAAGATTTAATAAAATCATTGATTATATTGCTTCAAGAAAGTAAAAAAATTCCAAATATGATTTTTGTTGCTCCAAAGGGATATGGCAAATATTCTTTAGCGAATATAATTTCTCAATTTAAGGATAGAAGTTTGCAACCTATAACTAGTAATAATATTGTTGATGAATTTGAACTGTTAAACATACTAGCAAATTTAAAAAATAATGCAATCTTATTCATTGAAGATATTAATAAATTAAAACCAGAGCTCAAAAAATTATTATTTGATATTTATAACTCGATAAATCTAGATAAAGTTGAAAAAATATGTTATTCAAACAAGCGTTTTAAGATTAAAAATTTTTCGATTATAGGGACAATAAATTCTTCAAATCAAATTGATTCCTCAAAATGAGAAGGCTTTAAAATATTCAATTTAAAAAAGTATTCTTTAAATAATCTTAATCAAATTATTAATTACAATAGGCTAAAAACTAATTTTAAATTAGGCAATAGTCTTGTTGAATATATTCTCAATAATTGTAATAATAATCCTGGAATATTAAATGAAGTGTATTTGAAAGCTATTGATTATTTTGCAATAAAGAATAAAGAAATGAATTTAAAACAATTAAAAAAATTATTAAATAAAACAAACAAAAAATATGAGAAAATCAATTAAGTTTTTTAAAGATATAGATAAAAAAATGTTTATTTGTGAAGCAAATTTAAACAAGGAATACAGTCCTAATAGTTTTAATTCAATATCTTCTTATTTAGCTATGTTTCCTGTTGAATTACCGTTATATTTTATAAAAAAATATACCAAGGAAAATGATACAGTTATGGATAACTTTTCAGGAAGAGGCACAACAGCTTATGCATCAAGAAAATTAAATAGAAAATTTGTAGGTAATGATTTAAACCCTTATGCATTTGTATTGTCAAAATCCAAACTTATAAATATATCAAATATAGAAAAGATTGAAAAACGAATAAAAGAATTAAAAAACAAGTATTTGCTTTTGCCAAGGTCTATAAATATAACGAGTGAAAAATATAATGATTTAAAAGTTTTTTATAGTGATTTTACACTTAAACAACTAATTTTTCTAAAAAATGAAATTGGCATTAATTGGAAAAATAACAAAGATATTGATAATTTGATACTTGCATTATCATTAGGACTTATGCACGGTCCAATGAAAAAAGATGGTACAACAATTTATTTTTCGTTAAATATGCCAAATACTATTTCAATGTCTACAAATTATGTTAAAAAATATTCTGAAATAAATAAATTAACTAAACCTAATGTTGACATATTCGATAATTTAATTAAAAGATTAAAAATTAAATGAGAAATTTTTCTATCTAAAAATTATGAGTCCATTTTTAAATATTGAAATTCATTAAATAGTTTAAATTTTTTAAAAAATAATAGTGTTGATTTAGTTATTACTTCACCACCCTATTTATCTTTAGTCGATTACACAAAAAGCAATTGATTAAGACTTTGATTATTAGGATTTGAAAAAAATAATTTAAAAAAAGAAATCAAACTTTCAGATTCGCTTGACTTAAAAGAATACACAAATTTTATAAAAAAATACTTAATAAATATTTCATCTAAATTAAAACCAAAAGCTAAAGTTTGTTTAGTAATTGGTGATGTTTATGATTTTGAATTAATAGAAAACATTTGAAAAGAAATTAAAAATGAAGTTCCTTATTCATTCCTAGAAGTAGGAATTGATACCAATACAAATAATTTTAAAACTTCTAAATTGCTTAATTCTCGTAAGGGCAAAGCAACTAAAGTTGAAAAAATTCTAATATTAGAAAAACAAAATTAATAACTATAAAAAACAAGGAGAAAATATGAACACACCAAATAATAAAATTAATATGTTATCAACCATACCATTAAAAATTAAAGGTCGAGCAATTATTGAAGATTATCTAAAATTAAAAGTTAATTCTATTCTATTTATGGCAGAAGATGATGAATGATTGTTTTTTGTTGCATGTTATCCAAAAAATGCTAAGGTAAAAAATGATAAAGAAAACTTAATTGACATAAGAAATAATCAAGTAAATTCAAAAGATGAATTAGATGATGTAAAAGAAATTTCATCTTATATTGACCTAAGTCATATTTTTAAGATACATGTAAAAAATTTAAAAGATTTTGTTAAATGTGCAACAGATGAAAGTTATCCATTTTTGAATTTACAAAAGCAACTTGAATATGTTGAAAAAATAACTAGTTTAACAAATGGCGAAAGATCAAAAGCGCCAAAATTAGTAAGAATAAACAAAAAAGGAGACAAGCTTATTGAAGCATAAAAGAAGGAGTTAATAAAAAATGACATACAATAAATTCAAAGAAAAATTATTAGATGTTTTTAAATCAAGAAGAGTCAAAAGTTTAATTGATTTGATTATGAGAAAACCAAATAAATTTTTATCTAAATCTAAACCTTATAATAGTAAAGAAATTATAAATTTAGAATATTATAGACTTCAAAATGTTGAGTACTTCAAGTTTTTAAAAGAAGAAATTAAAAATGTGTTAAAAGAAAACAAATATACTATTGAAAAAACAACTGCCAAAGATATAAATACAATTTTAAAAGGCAAGGAATATAAAAACATAGAAGTGCCAATTAACTTTGATTTTATTTTTAGAAAAAATGGTGATACTTCTTGCTTAACTGTGTTATGAATCAGAAAAAGTGATGGTAAATATCCTAAGAAAAGATATGAAATAGCTTATAGCTTATCAAAGCAATTAGAACTTATTAAATACTGTTTTGATGAAAAAATAATATTAAAAGGTGCTGTCGTTTTCATAAATGATTTTGAAAATAAAAATCCAGATAACTTTTTAATTCGAAAATATGATTCAGACAATGGAATGGACAAAAATTGAGTAAGTTGCTATTATGATAAAGATTTTTTTGAAAAATATAATTCTTTAAAATCTTGAAATGATTTAATGACATATTCAACTAATTTCAAAAAAGAATTTAAAAACTTATTTATTAAAGGAATTGATTTTGATAAAGATAAAAATGTTTTTGAAATCTTTAAAAAAATAAACAAAATCAAATTTTACAATTTCCTTTTTAAAGATGATTCTAAATTAGAACAAATTAGACAATATATATTTTCAAATGATAAAAACTCCAATAGATTAAAACTTTTAAGATATTGAAAAGAAAATTCTGACAAAAGAAGAAAGGAAAAAAATGAAAAAATTTAGATATTATACAGACAGAGAAAGTTTACTAAAAGCTGTCACAAATGATGGAATGAGAATTGAGTATTGTCTTCCAGAATTTAAAAATGATAAAGAAATAGTGTTAAAAGCTGTAAAAAATAATGGAAGAGCTTATTACTATTCATCTCAAGAATTACAAAAAGATGATGATGTTATAAAAGCTTCTTTTATTCAATCAGGAGATACAAAAATTTTTGATAATTTTTCTGATAAATGTAAAAACAATAAAAAAATGATTTTAAAATTGATAGAAAGAAATCCATATATTTTCAGGTATTTACCTGATTCACTTAAAAAAGATTTAAATGTCTGTTTTAAAGCTTTTAATATTCACCCGGCAGTTATTAAATTAGCACCAGATGAAATTTTAAAGAATCAAAGCTTAATTTTAAGATTAATCAAGAACAAAGAATGAATACTAAAATATTGTGGTGAATTAAATTCTAATAAAAAATTTATGGTTAAAGCTGTAAGTGTAAATGAAAAATGCTTGAAATTTGTTAGTGAAGATTTAAAAAACGATAAAGATTTTATCTTTAAAATAAGCAAAAATCTATAAATATTATGCAAGAAAAAGATCAAAATTCTGAAGTTTTTTCAAAAGAAAATAATACAATCCCAAAACAAATTCCAAGCACAGTTTATACTTTAGAAAGCACTATACAAGTGCCAATAACAAACTTTACTAAAAAAAGAATTTTTTGATTAGTTAATTTTTGATTCCTATTTACTTTAATTCTTTTTTGTATCATATTATTACCTATTTCTTTAGTGCTAGCAATTAATTCAGAAGATGTTTTACTATTAAAAATGTCTTCTAAAGCATTGCTGTGAACTTATACTATCATTAATGTTGTAATTACTGTTTTTGTTTTTTATAGATTTGTTTCTTTATGAATTATTGCATCTAAAATGCATAAGAAACATACATTAGATATACATAACAAAAAACTAGGTATAAAAGCAACATTATGACCATTTATTAAATATAACAAATTAATTAGCTAATTTACTAAAAACACTGTTTTATAGTATTTTTAGATTATCTTTAATTAAAGATTTATTTTAATATAAATATAAAGTATAATATTTATATAGCAAATAAGGAGCAATATGAAAACTAAAAAGATATGATTAAAGACAATATTACCAACAATAACATTACCAATTGTAACTGCTTTAACAGCAACTAGTTGTAATTGGTTTGCTTTTCCTATTAATCCACCAAAACCATCTAAAAACCCAACTATAAAAAAGGAAAATGCAGAAGGTTTTAGTGATAAAAAAGTTATTTCTGCCAAAAAATCTATAAGACCAGAAATTAAAGATTTTAAAAATATTGAGTTTTATATGGGAGATAAAAACTTAGCTAATCATGACGGAACACTATGTGCAAATGTTAAGAATTGATTAGATTTTCAAGTTCCTGGTTTCTTCTACTATGGAATATATTTAAAAACATTAATTAGTGGAAAATATTTAAAAGAAAACTTAAATGAAACAGACCTTTTATCTATTAACACTAGAAAAGAAAGATTAATTAATGAATTGAAATATTTTGAAAATAAAAAATTTGAAGTTTTAGACAACAAAGAAGAAGTAGAAAAAAACCATAAAAGTCCAACATATATTTATAAAAATTACATTGAATGTGTAGATGCTTTAGGATATTTTATGGAAAATTATAAAGATCCAGACTTAAAATGAAAGAAAGAAGATCTTTTTTTAAGTGATATTAACTTTTGCATTGAAACTTCTTTTAGTAAGAAAACTTGACCTAACTTAACAAAAGATGAATTCATAAATTTCTTTAAAGATAATGCAGTTAGATTTTATGATAGATTAAACAATAAATATGGTTACTCAATTAACTATGAATCAACTCATGGAAAATTGTCATCATTGAACTCAAAACAAATTCAAGCACTAGATAAAATTTATCTAATGATGGAAGAAGCAACAAGAGCTTTTTATAGAGTAAAGCATTTTGAAAATTTATGTAAACCATACAACACAATAGGTAATCAAATCAACTTATATGTATCAATGATTAATTTATTTAAAAACTTGTATAACTTAAAAATTGATACTAATAAAACATTAAAAGAACAATTGCAAGATTTAAAACCTGAAGTTTTAAGAGCTTTAGAGAAATCAAAAAATGCATTAAATAAATTATTACCTATTATTGATTCTGGTTTTGGATCGTGAAATTGAAAAGCATATACTAATATAGCTTTTCAACATAAAGCAGACAAACTTTTTGGTTCTAAAGAATTATCTAAAGATGCTCCTTTATATTGATTATTTGAAGCTCAAAATGAAGAATGAGATCATTCACAAGCAAAATTTATTGCTAATGAAGTTATGTATTATTTAAAAGAATTTCTTTTACCTTTAAGTAAATTATTAGAAGTAAACTATGATTTAGAATCTGTCATTTGAAAACAAATAGATTTCTTATATGACGTCGCTAAATTAGATGCTAGAACATTAACAAAATTTGAAACAAAAAATAAACCTGTTAATTATGCATCAGCAACTGATGAAGAATTAACAAATAAATTTAAACAACTCCAAGATACTTATAAAATTTTTGGAGATATCTCAAGCTCAGACTTGAAAATAAAATAAAAAATACAAAATAAATATTTAGTTTGATAAAAAGCAAGAAAAATACAAGCACTATTTCTGATAGTGCATTTTTTGCTTTTGGAAAGGAAAAAATGAAAATAACATCTTTATATTATGCAAACAATAATCCAGGAATATCAGTATTAAAAAATACATCGACTTATGTACTTGATGAATTTGTAAAATACTCTGCTATTATTTTTGGAATTTTGTTTGCAATTGCATTTGTAACATCAATAATCACAATTGCTATGAATGCTGCATTTACTTCAAAAGGCAACAATACTCCTGCAAAGCTAAAAAAGGCACTAAATGGTTCTTTAATTTCTTGTGGTGTTCTTTTAGCATTATTAATTTTGGGGCCTGGACTATGTGGTTTAATTTCTAATTCATTGAAATAATTATGTTTAAAGACCTTTTTTGATGGCTTTTATTTAAATTATATCAAGCCCCTTGATTTATATTAATTGGTTTACCCTTATTTATTATTAATAGTTTATCAAAATTAATTAATTTTGTTTCTTTTGATTTTCTTAATTTAATGATTTTTGGTAAACCACTTTCTAATATTAATAGTTATGAAGATTTAAAGGTATTGCCTGGTGCATTTATTGGCATAGCTATATTTAGCATTTGTTTATTTTTTATAATCTTAGCAATTAGTGCTTTTAAGCTTTCTTATAATAAAAGTAAAGAAAGCTTTCAATCATTTAAAGCAAGTTTGAAAGCTATAATACCTAATTTAGTTTTTATAATAGTAATACCAATTTTAATTTGAGTATTTACATTTTTTGCAAAATATGGCATTAATGTATTAAACACTGTAATTAGTGGTGGTCAAGAAACAAATGAACTTGGTAATTTAATTTATCAATCAGTTGCTCCTGAGCCTAGATTTATCGATCCATTTTCGATTACTTTTGAAGAATTTATGAAAGATGAGTTGAGTCTATTAAATTCAAATATGGGGAGCTTATTTTTAAATAGCTTCATAGCTATTTTTGCTTTATATATTGTTGGCAATATAACAATCAATATAAGTATCAATGTTTATCAAGCTTTTTTCTTATTTATTCAAGCTCCTTTAATTAGTGTTGTTTCTATTGAAGATGGTGGTAAAAAATTAAAAGTTTGAAAACAACAATTCTTTTCAAAAATGTTAGCAATTATGATTTTGAATTTATCAATTTCGTTTATGGTCTTATTTTTTAGTGGCGCATCAAAAATCATTAAGCCTATGTCAGATGCAACAGGTATTGATAGTGGCATTTGTGCCTTGATTGTTTACACAGGTGCATTAATTACTTCATTATCATTAAATAAAGTTATGTTTGGCATATTCGGAGTTGAATATACAAGTTATCTAGGGAAATTTACCAAAAAATTAGCTGCAAAAGCAACAAAAAAAATTTCAAAACGTAAATCTAATTCAAAGAATAATTCATCTTCAGAACCTGAAACCACTTCGCAAACAGATTATCATCCTACAGGGAACGCAAAAAATGCTGAGTTGATTATAATAATGCAAAATAATAATCAAACAAAAAAACCAGAAACCACTCCACAGACTATAGGTGCCGCAAAAAAAGCTAAAAAACCCGAAACTCTAAATGACTGAAAAAAAGAATTAGCAAGTACACAAAAACAAATGGATCAATTGTCTAAAACTCTTCGAGGTGAAAAAGACCTTAAAAAAACATCCGAAATAAGCAAAGAATTAAGTGAGTTAATTGAAAATGAATCATCCATTAAAAGAATAATAACAAGCATGGAAAGGAAAAAATAAAAATGAATCAAAACAAAAAAATATCATCATTAAAATTAAATATTTGAAAAAATTTAAATATTTATGATGCAATAATTATTCTTGGAATTTCAGGTTTTTCAGCAGCTTTAGGCTTTGCTAATGGATTGCCTTTGTGAATAAAAATCATTATATTTTTAGTGGCTTTTTGTTCTTTAATTTGGTTAATTATTCCAAGCAAAAAACATAACTGTAGATTATATGTTTTAATACTAAGAATATTAAAATTTAAATCTTCAGTAAAAAAATATAAATTAAACAGTTCAAATGACACAAAACTTTTAATACCATTTAAAAGAATAATTGACAATAAATATGTTGAAACATTTTCATCAGTAGGAAAAGGATTATTTACAGTTTTTAAGTTTTATGGTAATAATATTTATTCTAATAATAGGGATGATAAGGTGGCGTTTTTTTCTCAACTAATTAATGTTTTAAATTCAATTGAAACAAAATTCACTTTTGTAAAAAAAACTTCTTCAGTTAACATTGATGACAATATTAAAATTAATGAAAATCTCTATAAAACAATTGATTTGGTAAAAACCAATGATGTTGTAAAGAAACATTTCATTTATAGTTAAAATGATTTTGAAAGTCTTAGAAATATTGAAAACATAGATGTCTATTATTTAGTTATCTATGCTAATGAAATGAATAAACTTAAACAATATGAAAATGAATTTATTAAGTCTTTTGAAACTACAAAAATACCACTTAATGTTGTTACTGGTATGGAATTAATAGACTTTGCTAATATGATTTTTAAAAAAAATATTTCAAAAAGTGACAAACAAAATTATTTTGTTGAAGAATTGGATACTGAATTAACAAAAGAACTTGATATGAAAATGCAAGGTCAAAAGTTAAATGATAAAGTAATTAAAAAACTTCAAAGTGACAAAGAATACAAATTAATTTCTTTATTAGCACCAGAAAGAGTTGAATTTAATTCCAAATACATAAAAGTTGATAATGAGTATTTATCATTTCAAACTTTAGTTGATTTTAACTCTTTACAAATTAATGAAGGTTGAGCTTGTGATTTATTTAACAGTGAATCTTTAGTTATTTGAAACTTAAAACCTATTAATGAATCCACTAAACAAAAAATACTTGATAAAGCTATGTCATTAAATGAAGCAAACGCTGAAACTAAAAGTAATTTTAAAATGCAAAAAATCAGTGTAGAACAAAAAGTTCTAGATGAGTTAGTAGATTTTATTAATGTTCAAAAAATGAATTTATTTGATTCTAATATCATTTTTGTAAATCAAAGTAAAACCTTAGAGGGAATAAAAGATTTAGAAACTAAAAATGATATAAATGCTAAAAAAGCATTTGCAAATGTTTATAAAATGCATTATTTACAAAAGCAAGCATTGCAAAATTCAATTTTTATCAAAGATGATTTTCTAAATAATGGAATTGAAATGAGTTCAAGAAATATAGTATATGGTTGACCTTGAATTTCTAATTCTTTAAATGATGGTAATAACTTTTTATTAGGTTTTACAACAACCTCAAGTAGTCCTGTGATTTTTGACTTATTTAAAAAGACAAAAGACAGAACATCATTTTCAATGATAATTTGAGGTGTGCCTGGTTCAGGTAAATCAGCAACTACCTCTAAAATAGCTTTGCATCAATTTGTAGTAGGAAATGAAGTTATTATCATTGACCCTCAAAGAGAATACAAAGACTTATGCAAAAAATTAGATGGTAACTGAATAGAAATGGGTAAAGGATTAGAAACAAAAATTAATCCATTACAAATCAATTATGAAATTGAAAAAGATAATATAAAAGTCAGTGATGAATTATTAATTAGTAATCATACAAAAATGGTTAAAGAATTTTTCTCATTACTTTATCCAGATTTAGATGCAAGAGATTTAAGAGTAGCTAATTTTTTATTAAATGAATTTTATTTATCTAATAAGATAAAAAATATTCAAAAATTAAAAAACACAGATTTTCCAACAATGAAAGACTATATCAAATTTTTAAAATCATATAAATTTAAAGACAAAAAAGCAGAGCTTTATTACAAAGATTCATTAAATAAATTGTCAATAATTTTTGATCAAGATTTCAATAATAATGGCATTTTTTCAAATCTTTATAATGGGCATACAAATATTAATTTTAATAAAACTTTAACAGTAATAGATACTTATAACTTAATTAATAACATTGCTTCAAATGAGGGACAAGCATCATTATATTTGATACTCTCATATGTTAGAAACAGAATATCAAATAACTTTTTTGAAAACAAAACTAGAAAAATTTGTCTTATTGTAGATGAAGCTCATAGATTTATTAATGCTAATAATATGTCATCATTAGAATTTTTATTTGACACAGTCAAAACAATAAGAAAATATAATGGTTCTGTCATTCTAACTACACAAAATCCAAGCGATTTTGAATTAAGTGATGAAGTTATGAACTTAACTAAAGGGATGGTTGAAAACATACAATACACATTAATTTTTAGACAAAATCAAAATGGTGTCAATGCTATTAGTCAAATGTACAAATCAAAAAATGAATTAACAAATTGAGAAAAGAATTTTTTAACATCTGCACCTATAGGAAATGGAATGTTTTCAATAGATAATAAAACAAGATTTCAAATTAGTATTTATTACAATCAATTCGAAAAAGATATGATTTACAAATCTGGTGATTTAAGTAACATTTAAGCAATAATACTATAAAACAGTGTTTTTGGAGAAAGGATAGTATGAAAAAATTAAAAAATTTTTTGCTTGGTATTTCATCTACAATTCCAGCTTCTGGAGTATTTTTGATATCAAGCACTAATTCTAAAAATGAAGATAGTTTTAATAATTGAAATGAAAGTATTAAAGATAAATGCCCTATAAAAAATGCTAACTGAGAAGAAATATTTGATATTTTAAAATCTAAATTACCATACAAAAAAGATATTGAATTGGTATCTTATTCAGGTATGAAAGATAATTTAAATTCAAAATTAAAACTAAATAAATTCAGTGGTGAAAAAACTCCTAAAGAAGAATTACTTCAATATTGAAAAAATCTTGACATTAATTTAGCACTAAATAATTATTTAAAAAACAAAAGCATTAATAAAAATGAATTTTTTAAATTTGATTTTGAAATTGTAGAAAATCAATATTTAAATATTTATTTAATTAATGAAAATGTTAATCTTAATAAAACAAGTAGATATTTATTGATTAAAAATCAAAAAATAAAGTGAAAACTAAGTTCTGATATTTCTCAGTTAAATTGAAAAGAAAGATTAATAATAAATTATGGATATAGTTACAAAATAAATAATGATGGAACAATACAAATAAAGCAATCTAAATCAAAAAATATAACTTCAAATGAAATTATTTGTGAATATCCACCAACAGAAATTTATTTTGTTTCACCACAAAATTTACAGGAAAAATTTGAAATTAGAATTGAAAGTGAAAATGGCGAAAAAATTGTTACTTTAAACAATAATGAAATTTTTTATAGTGCTATTGATATAAGGAAAATTGTTAAAAATAAATTAAATTTTTCTAATAAGCTTTTCTTAATACATGAAGATTCAAATGGCATTAAAGAAAAAATTCAAATCAAATTTGAAAAAAGTGATATATCAAACACAATTGATTTAAAATATCCGAATTGAAATCCTGATTTATTAGAATACAAACAATTTATAAGCAAAAATGAATTAAATAAAGATGGCAATGAAATTAAAGATGCTAATGGGAATCCTGTACCATCAAAAAAATATGACCCTGGGATCGATCCTAAAACAGGACTTAAATCAGAAATAGTTTGATTAAATTTTAATGAATTTCATGATTTTTTGAAAGAAAACATAAAAAACTATTGTTCTGTCTTAATAACAGAAAAAGAATCTAAATTTCTTTACGAATTCATGAATGATAAAAAATTCCGCTGAGTTGAGTTTTTAATAAATTATGAAATTTTAAAAGAAGAATTTGATCTTTTTGATAGATGAAATAATGAGGGCATTGGCCCATATCCTAATGAGGGGCGCTACATTGGAAAAAATATTCCTTATGATGAACAAGAAAAAAATATCAAAATTCTATATGAACTACGAGATTTATATGCCAGAATACAAGATTCTGGCATTGATAAATCTGAATCAATAAACATAGATAGATATTTCGAATTAAAACAAATCGTAAAAAAATATAAAAATCATTTTGATTCAACTAATTGTAGATTGCCTTTTGGTTCATTTTATTTAAAATATAAAAGTAATAACTCATCTGTATTAGCAGAAGCTATTAAGACTCAATATTTGACTATTAATGCAAATTCAAATATTTTAATAAGAGAATTAAAACAAAACTCAATAGGAAAATGAGAATTTGTAAATGATTTAGAAAATATGGAAATATCTAATTTAGCTCCAGGAAATGAAATCAAATTAACAAAAAATGGTTTTTATTTAATATATGACATATCACTAGATAAAACAAATATTAATAAATTTAAAATTGTTTGAATAGATAATAATAGTACTACATATACAAAACCTAGTTATTTAACAGAAGAAGCAAATTCTCCATCATTTATCAAAAAATTTTGAAGAAATAAAAATCTTAAAGTTTCAAATGATTTTGAAAAATACTTAGCCAAGAAAAAAATTATTGATTTAAACGCAACAAAAAAAGATCTTGACAAATTAAAATATGATGATATTTTTAATGAGTATAATAATTTTTTAATTGAAAAATCTTTTGAAGAAAATTTTGAAATCAAACCAGAAATTAACATTAACAAATTAAGACAAGATTTTAAAACAAATGAACAATTTTTAAATTATATAAATCAAAAGGATAATGAAGCTAAATTTATTGAAAATTATGTTATCTCAAAAAATAAAGAATTTTTAACAATTAAAAACTGTTATTTTGATTCTAAAAGTAACTTAATTAAAGTTGTTATTGATTTAAAAGAAAATGCTAGCTTAAATAGTTTTTTTATTAGAAGAAAAATCTTTAATATCAATTTAGAATTGAAACCTATTTTTATTAAATTTAATAATATTAATATTGAAGAACATATACCATTCAATGTATTTGTTAATGAGTTAATTAATAATTTTAATAATTTGATTGAATCTGCTAAAGATATAAATAATGAAGACATTGACTTGAATATTATCGAATTAAAAATTTTGAAAGAAAGTGATGAAAATCAAATAATAGTTAAAGCTATATCAAAAGATGCAAATTATTATATAGCTAATAACGATACATATACATACAATGTAAATTTACTTAAATCAGATTATAGCTATTTATTGAATTTTAATCCTGGAACTTTAAATGTTTTTGGTTTAAAAGATAAAGAAGAAATAAAAAAATACATTAAAGAACTATTAAAAAATTATTTAATCCAATACAATGAAAAAATTGATCAATCTCTTTACAAAAAAATTACAATAGATGATATTAATATATCGTTTAATGATGAAGAGTTAAACAAAATAATTAATCCAAAAGATGATGACATTTATTGAAATAAAGATGAAAATATTGCAAAATTTACAATAAACTTCAATAATAACTTTAAAACTTATCATCCTTGCACACTCCATGTTATCAACAATGCTCTAGATGACGATTTTAATTGACCAGAAGCAGGCTTAACTGTAAAAGTGAATGTTGATATGGAAAACAAAAAAGACCCTGTTAAAATGGCTCAAATTAAAAATGAGACTATTAGACAAGTTAGAGAAAAAATAATAGAAGAATTTAAAAAGAGAGCTCCAAAACTTGAAATTGATAAGTTATATACTATTGATTTTACCGATTCATTTTTCAATAATTTAGATTTAGCTATCGGAACAACCTTAACTTTAAAAGGCAAGCATTTTATGTTGAAAAACCCTTATACAGTTAATGTTATAAATACTCATTCATCAGCAAACAAAAAATTAAATTTAAAAGACTTAAATTTTGAAGTTATTACTTTAAGTGAAAAGAATTTAAATGATTTCAAAAAAGCTTTAAATGAAAAAATTATTGATATTCTCAAAAAAGAAAATTATCCTGCTAAAGAAATAATCAAATTAATTGAATTTAAATTATTAGAAAACCAAAAGTGAGATTCTTTCTTTAGTAAAATTGGCAATAATGAATTAAAAATGATGATTCAATTAAAAGAAGAATCAAATTTAATAAGTGGAGATAAAGAATTTAAATTAATTAATATTTTTACAGAAGAAATGTTAAAAGAAGCTTTAGAAATTAAGTCTAAAAACAAGAAAAAATTACTTTCATGACTAATTCCAGTAATTATTTTAAGTGTTGCTGGAATTATTGCAACAGTTATTGCAATATGATATAGAAAGAAGAAGAAACGTTTATAGATTAAGCCTATAGGCTTCTTTTTCTATTTCTGTGATTTTACTAAAAATACTATAAAACAGGCTTATAATAAAAATATGAATTTACAAGATAAGCAAAATTATTATGAAAGTAATTTGAATTTTTCATTAAGTTCATTAATTATGAATGATGAAAATTCATTAAATAAATTCATTGCTATTAACTTAATCAAAAATATGATTAACCAATTAATTAATTATAAATATAGTGATGAAAGAAAAAGTAAATATAAAGCTCATGATGTGATTATAAGAATTGTGAGATTTGAAAATTTTACATTTAAATTGAAAGTAGAAACATATAAAGATAAAACAACAAATAAGTTGCATACATTTTATCCTGATTATTTAAATTTTGGATCATCTATATGTGAAGATGAAAAAGAAAGAATTCAAAATTATGCTGGTTAAGATAGAATCACTTATTCCAGAATTGCGAGCAAATACAATGTAAGCAAAACTCAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCCCTTCCCAGGGGAAAAAAAGGTATTTGAAAATAAGATATTTCCCTTTTGAAAGTTTTGGCAATGTTTCAAGCAATTAAGAGGAATTCAAAAATTGGGGATTTATGCTTTTATGTTAAATTTAGGGATGGTGTCCTTGATTCAAACCTAGCAAACACTAAAAGGCATTTTTTTTGGGTAATTTCATTTTTAAGAAATTTAAAGATTTTATTTTCAAAAAGAGAATCGTGGATTTAACAATTTTATAGATAGCATTCAAATTTAAGTGGGTGAAAAATCGAAAAAGAAATATCAGAAGCTTACAACAGTAGAGATTATGGAAAGTTGTTAGATATATTAAATACAATTTATCTCTATTTTAGAGACTCTTTTGTTTTGAGCGATATTGATAGATACAATTTAAATAGTTTGATAAGATACACATCTAAACTTGAAAGTGGAATCATTAATAATGATTATAGTTCACTAATAGAAGGACACATATCTGTTTATATTAAAAGAAAACTAAACAAAAGCTTTGCATTATATTCTCAAAAAACAATAGAAGCTTTATTGTTGAATGATAATAAAATTCAAAATACTCTTATTCAGACATATCATTAGTATTTATATTAATATTAATATAGAAAGGCAATTAAATTTGCTATTTTTTCCACGCAATTTTCTTTGTAATTTACATTTTTTGATATAAAATATAGTTGATAGAAAAAGGAAATGCCGTTTTCTCTTTTAAATTTCTAACTTATCCCACTAATAATTTACATCACCTTTAAAAATGCTTTAAAAGCCTATAAAACAATTGAAAAAGTTAAAAATAACAATGTAGAAAATTTTTTAAAAATACATAAAATTCTTATGAATAATATTATTAAAAGTGCTGGAAAAACTAGATCTGGAAATGTTGGGGTTTATGAAAATGGAACATTGATTCATATGGCTCCACCAGCTAATTTTGTAAACAATCTTTTAATAGATTTATTTGAATGATACAATAATGATATTAGTGATGTTTTAATAAAAAGTTGTGCTTTTCATTATGAATTAGAATTTATTCATCCTTTTGAAGATGGTAATGGAAGAATAGGAAGATTGTGGCACAGATTTTTATTAAATAAAGAAAATAAATTATATGAAAATTTACAACTTGAAAATATAGTTTATGAAAATGTCAATAACTATTATAAAGCTTTACATAACTCAAACCAAAAAGGAGATTGCAGTGAATTTATTGAATGCATGTTAACTTTTATTTTAAAAGCTATTGAAAGAAATGAAATCTATGATTTTGATTCATTAAATGAAGTAGATAAATTGACTTCAATATTATCTTCAGGCCCTAAAAGTAGACAAGAAATTATGGATTTATTAAATATAAAATCAAGAATCACATTACAAAAAAGTTATATAAAACCGGCTTTAGAACAACAAAAAGTAATAATTATTGGTAAATTAAACTCACCTAAAGTAAAATATAAATTAGTGGAAAATAATAAGGAGTAACTAATGAGCAAATTTATAATTGATAAAAGTTTTTTTGAACTTTTTCCTCAAGCTAAACTTGGAGTAGTTTTAATTAATGATATTAAAAGTAATTCAAACAGTCCAAAAGAGCTGCAAGACTATTTAAGAAAAAGTACTTGATTAGCTAAGGACCACTTAACTTCTGCTATTTTTTCAGATAATGAAGTTGTTAGAATTTATCGTGATGCATATAGTAAATTTAATTGTATGAAAAAAGCAAGATCAAGTGTTGAAATGATGCTTAAAAGAGCAAGTAAAGACGCACAATTTTCTTCAATTAACCCTTTAGTTGATATATACAATAGTGTTGGCTTACGTTTTGCATTTCCTTGTGGCGCAGAGGATATTAATAAATTTAGTGGCAATTTAATTTTAGGCATTACCAAAGGTGGAAATGAATTTTATAGAATTAATAGCACTGAAAATGAACCTGCTTTAGCAGGAGAGTTATGTTATTATGATGATAAAGGTGCTGTTTGTCGTAATTTTAATTGACAAGATGGAGAAAGAACCAAAATTGATGAAAATACTAAAAAAGCCTTTTGTGTAATTGAATTATTAGATACTAATAGATTAAATGATTTAAATGAAGCACTAAAAACATTAGCTCAATTATTTAATAAATATTTAGGAGCAAAATGTACTATTCATTTATTAGACATTAACAATTCAAAAATTGAATTAATTTAACAAATTGTTATATTTGCACTTAAAATATGCTTAAAATAAGTGTGGATATAACACTTTTTATAATTTTTTTTGTTCTACTTACACTTAAATGTGATAAAATTTAAGTGTGAAAATAACAGGAGGTAAAATGAACTACAATGAAAAACTTGAAAAAGTTATTGAAGAATGTAATGGAGTTGTTACTACTAAAGAATGTAATAAAAGAAATATTCCTAATTCAATAATTTCAAGAGCTGCCAAAAAAGGCAGAATTGAAAAATATTCTGAAGGAATTTATGTTGCTGAAGAAATACCAATGGATCCACACTTTCTTTTTCAACTTCGATTTCCAAGAAGTATTTTTTCTTATGAAAGTGCATTGCAACTTCATGGAGTGAGTGAAAGATTTGAAAAAGCGCTAGAAGTCACAGTAAATACAGGATATAAATTTAATGATACTAAGAAAATCTTTCCGCACTATGTAAAAGATGAGTATCTTGAATTGGGAGTAGAAGAAATAAAAACAAGTCAAGGCAATAAGGTTAGAGTTTATTCTTTGGAAAGAACTTTTTGCGATATGGTAGCTAATAGAAATAAAGTAGATGCTGAAACTTTTGTAAAATTTATGATTACATTCCAAGGAATAAAAATAAATTATTCTCAACTTAATAGTATTGCTAAAAAAATGGGAGTCATTAACAAAGTTGTTGATGTAATGGGAATAATTCGTGAATAAAAATAAATTGAAATATTTGTGTTCCTTGGTTTCAAAGAAATTTGATATTGATTACAATTCCATAAGAAAATTATTTTATCTTGAAGAAATACTAAAAAGAATTGCAAAAAGTAAATATAGAAATAATTTTATTTTTAAAGGCGGGATGTTGTTTTCAAACATAATAGGTATTGAAAATAGAAGAACTGTTGATATTGATTTTAGTTTGAAAGGTATAGAGTCTAATGAAAAAAATTTAAAAATTGTTTTTAATGAAATATTGTTAATAAAAAATGATGAAATAAATTTTATTTTTAATGATATACAACCTATAAAAACTGATTTAAATTATCCTTGTTACAGATTTAAATTTAGAGCTTCCTTAGAAAACGAGAAAGAAGATATTGTTCTTGATATTGGTGTTGGAGATAAAATATTTCCAAAACCAATTGAATACTCTTATAAAAGTTTTTTTAAAGATGAAAGTTTTCAAGTTTTGTCATACACTCTTGAAACATATTTTTCAGAAAAACTTTTTGCAATAATTTCTAAAGGAGAAGCTAATACAAGACTAAAAGATTATTATGATTTACATTTTTTAATATTAAATTGTAAAAAAATGATTGATAAATCAAATTTAGGCAAAGCAATTTCGATAACATTTGATAATAGAAATGAAAAATTTGACATTAAAACTGCAAAATTAAAACTTTTAGAGTTATCTAATTTTGAGTTATTGGATAATAAATGAAAAAAATATTTAATCAAAAATGAATTTACTTTAAATATTACTTTTAAAAATTTAGTTTTAATGATTGTTGAATTTTTAACTGAAATTTTTGAATAAAAAAGAGTTCACCATTTAAAAGTGAGCTCTAATTTTTTTACAACTTAATTATTTAATGTTGTAGAATGCTTCACGACCTTCAAATTCATCAGCACCATCTAATTCTTCTTCAATAGCTAATAATCTATTGTATTTTGCAATTCTATCTGTACGTGACATTGATCCAGTTTTGATTTGTCCTGTGTTTAATGCAACAGCAACATCAGCTAATGTTGTGTCTTCTGTTTCACCTGAACGGTGTGAAACAACACAAGCCATTCCAGCTTTTTGAGCCATGTTAATAGCATCAATTGTTTCTGTTAATGAACCAATTTGGTTTAATTTAATTAAAATTGCATTCATTGCTTTTTCTTTAATAGCACGCGCTAATAATTTAGGGTTTGTAACTGTTAAGTCATCACCAACGATTTGAATTTGGTGCCCGTATTTAGTTGTCATTTTTGTAAAACCAGCTCAGTCATCTTCTGCTAAACCATCTTCGATTGAGATAATTGGGTATTTAGCAATTAAAGTACCATAGTATTCAATCATTTCATCTGTTGTGTATTCAAGTTTAACATTTGTAAGTTTTTCAAAACCTGGGTGTTTAGCTTCAATAGCTTTTTTAAGTTTTTTGAATACATATTTTTTGCTTTTTTCGTCGTAAATTTCACTTGAAGCAGCATCCATTGCAATAGCAACAGCATCTTTACCGCTTGTAGCTGGTTTGTAACCTGCAGCCTTAATAGCTTGAACTAAGTAGTCAAGAACTTCTTCATGACTTTTGCAGTCAGGTGCAAATCCACCTTCATCACCAACTTGTGTTCCGTGTCCTGCAGCTTTTAATAATTTTGCTAAGTTATGGAATACTTTGTTTGCCATTTGTAATGCTTCACGAATGCTTTTAGCACCAACTGGCATAATCATGAATTCTTGGAAGTCAACTGTGTTTGATGCGTGAGCTCCACCATTCATAACATTTAACATAGGTAATGGTAATACGTGGGCATTTGTACCACCAATGTATCTATATAAAGGAAGTTGTAATTCATCTGCGGCTGCTCTAGCTACTGCTAATGAAACTCCTAAGATAGCGTTAGCACCAAATTTTGATTTTGTTGCTGTGCCATCTAATTTAATCATTTTCATGTCGATTGCACGTTGATCTGTAACTTCCATTCCTAATAATTTAGGTGCAATATCTTTGTTAACATGATCAACTGCGGTCATAACACCTTTACCACCAAATCAGTTTTTTTCGTATTTGGTTCCTTGGTCTCTTAATTCAAGAGCTTCTTTTGAACCTGTACTTGCTCCTGAAGGAACATTAGCTAAACCATATCCGCCTGCTTCTGTTCATACTTCAACTTGAACTGTTGGGTTTCCACGTGAGTCTAAAATTTCACGTGAATGAATCTTGTAAATTGCTGACATTTTTCTCCTTAATTTTAAATTTTAATTTTATGTTTTATATTATACAAAAATATTTATTACTATTAACAATGAAATTTTGCTAACTATTTTTTATTATGGAAATATTACTCATAATTTTCATTAATTACAAAATGTCAATATAATTGTTAAAAATAGGAATTAACAAAATAGATAAATTTATTTTGAAAAAAATCTTAATATATATAATATTGATATTAATTAAATAGGAGAAAAATGGCGAAAAACCCAATTATAAATAAATGAAGCAAGAACTCATCTAATTATGATGACTTTATGAAAAAATTGCTTACTTTTGGTATGAAAAAGGAAAATCAAGACATTGATTCTGCTTTTTCTAGACCATTGCAATTTATTTCTAATAAAGTCATTGCAAAACTTGGAGTAGGAAGCCATTTAATCAATGATTATACTGTTACAGCTTTAGGACATGCCTTCTGTGACTCAATTGTAGAATCTGAAAAAAATAATAAAGAATTCAAAATATTTTTATCAAATGATAACACATTTCATGCTTTACTTTATACAAATATTTTGGCTCGTATTTTTGACGAAAAAGGTTTTAAATCATACATATTTGAAAAAAATAGTTCATATCCAAGCGTACTAAAAAACTTGGCTGCAAAAGACGATGATTGTGATGTAATTATTAGTGTTGAAAGCTTTAAAGGTCTAAAAAATATTATGCAAATTAGTTTCAATTGAGGCGATGGTAGACCATTTAACCAAGTTGAAATTGCGAGAATTTTATTTAAATTGAATTCAGTAAATTATTTAAGTATAGACATTCCAAATCAAGGAATTAGTTTCAAATATAATCACTCTCCTTTAAATTATAATCGCGAAATCTTAAAACGTTATTCACCTTTAATTAGTACATTTAAAAAGCAAAATTTAAAATTCGGTGTTGATATTTCACAACTATCAACAGCTAATTTTTACTATGATACTTTAAATAGATTACAAATTGACTACTTTACTCATAATCGTAAAAAAGATGCTTACATTATTAATGCAAATAATCCAGATTGTTTAAAAAAAATATTCTGAAAATCTTTACTTAAGAAACCAGATGCCAATTTTGCAATCAGTCAAGATGGCTCAGGAATTAATCTTTCTGTTCGCTACAAAAAAGTTTTTAAATATTTTAAACCTGATGAAATTGCGGCTTTATATTTAAACTTTTTAATAGAAGATGATCCAATTTTTGATAAAGCAAACTTTGCTAATAGTTACATTATTAAATCTCTTGGAGCTGGCAGTTTAACAAGTTTAATAGCTTTAAAACATAATATCGGAGTAGAAGAAACTCCTAAAGTTTCTGGAATTTGAGAAATCGCTAATAGAAATAGTATTAAGAACAAAAAATTACTTTTTGCTTTTACAAGATTTAGTCAATTTGCTCCTTTTAATCGTTTTTTCAATGGCTTTGACGCTAACTTATTTATGTTAGAGTTAATGAGAATGATTGCTTTCTATAAAGAAAAATCATTAACTCTTTATGAAGTTTTACAACAAATTTATGATAAATATGGATTGCATCATGTTATCACTAAAACATTTAATTTAGATGATGAAATGGCTTCGAGATTTATTAAAAGAATACTTAAATCTGAAACAATTGGTAACCATAAAATAGTTAACTTTAAAGAATACAAAAATGCCACAATAGTCAATAATAATATCCATTTAAAGCTTTCTTTTGAAGGTGGCAATAGTATTACTGTTAAGTATTCTATAATTGAGAAAGAAATAACTATAGACTGTGAAGCAATAGCTAAAACTAATAGTCAAGAAGATCGGACCAATGTTGTAATTTTAGAAAGAGAACTTATGGATGGAATTTTAGAATTAAAAGAGGACTTTAAAATTCGCAAAATTACACCATGAACATTTATTAAATGATTCCTATTTATTATGACTTTTATTGGAGTCATTATCTTCCTTTACTTTAGTATTTATAACTTCAAAGATGATAGTTTCTTTGGGGCTAATGGTGATATGGGTGAAATGTTCAAGCGGATGGGAATAGTAATTTTTAAAGATCACAAAACGCAGTTTGCATTCTTATCAATTGTTCTTTCGTTTTTTATTTGGACCTTATTTAATGCTATTATCTTCAAACGGCTTCTCGATTTCCAAGGTCAAAAAGTCAAATGAAATGATCTGATGATTAGCTCGCTGATTTCGACAATAGTGCAAAATGTTACACCTAAATCAATTGGTGGCGATTTAGCTACCTATTGATACTTAAGAAGAAAAGGAGTTAAACGTTCAACACTTTTACCAGTTGTTGTAACTAATACTTTCTTATGACAAGTAACTAATGTTATTATAACTGTTTTCTTCTTACCTTTTGGTATTTGATTCTATAAAGACTTCTTTACTAATATTGATAATCCACAAGTAAAAGTTTTCTATGCATTCTTTATTACAGGATTATTCATGGACTCAACTTTTGCTTCGTTCTTCTTCATTTTGGCATTTAACAAAAAAATCCAAAATTGATTATTAAAAGTATTTATTAAATTCCTTGAATGACTACCATTTGTTAAAATTTATGATCCTCTTTTAGTTAAGGCTAAATATGAATATGAATTTTATGAAATGCGGCAAGGGATGAAGAAAACATTTAAAAAATGATATCAATTCGCAGAAGTAATAATTTGAAAATTATTGCCTAACTTCTTCTCGCCAATAGCCTTCTTTATGAAAGCTTCTGGAGTATTGCAAACTAATCTAAAAGGTGGCTGATACTTTAATGTTCTTGTTTCAAATACAATTATTCGGATAGGAAACTCAGTAAGTATAACGCCTGGTGGTCAAGGCACAAATGAGATCTTTACTAATATGATTTATAAAACTATTATGAAGGATCTTAGCAATGCAGGTGAATGAGTTTACCGTGGAAAAGATTATGGACTAAATCAAATTGGTAACGCTAAATTAATGACAACTATTGGCTCAATTTGAGGAACAGTTTTAGGTTCAATTCTTTCTGCTATTTATTTGATGCTGGTCTTCATTGGTGAAAAGAGAGTTGACTTATATCAAATTAAAGAAAAGAATTTAAGACTTATTCAAAATGACAATATGTCAGCAACCACTAGAACAAAAACTAGATTTTACAAAATTAGCTTTACAATTTATACAATAATTATTATTGCTTTAGCAATTACAATATTATGCCCTCTAATGGGATCATATAAGTAGGTACAATAGATGCCTATTTTTTTGTACAAGCCTGTTTTATAGTGCTATTGGAGAAATGACGCGATTTAAAAAAGCAAAAAATTTTAAACAAATTAATAAGACAAAAAAAGTTCAAAAATTATTTTTGTAAATTTTTAAAAAATATATGTAAAAGCATTTTATTATGCTAAAATATTAAATGCTATTTATAAATATTTATAAAAGTTATATTAGTAAATAGTATGGATAGGATGCAGATGAAAGACTGCACATCGGTTGGTTTTAGCGCTAAAATACAATTTATTAATAAAATGAAAGGAAAATATTACATGGCAAAACAAGATTTTAATCGTAATAAAGACCACGTTAATATTGGTACCATTGGTCACGTTGACCACGGTAAAACTACTTTAACCGCTGCTATTGCTACAGTATTATCAAAGAAGGGTCTTGCAGAAGCTAAAGACTATGCTGCTATTGATAATGCACCAGAAGAAAAAGCTCGTGGTATAACAATTAATACATCACACATCGAATACGAAACTGAAAAACGTCACTATGCACACGTTGACTGTCCAGGTCACGCTGACTACATCAAAAACATGATTACAGGTGCTGCACAAATGGATGGTGCTATCTTAGTTGTTGCTGCAACAGATGGTGCTATGCCTCAAACACGTGAACACATTCTTCTTTCAAAACAAGTTGGTGTTCCTCGTATGGTTGTTTTCTTAAACAAATGCGACATGCTTAAAGGTGAAGAAGAAATGATCGAACTTGTTGAAATGGAAGTTCGTGAATTACTTTCAAAATATGGATTTGACGGAGACAACACTCCAGTTATCCGTGGTTCAGCTTTAGAAGCTCTTAAAGGTAACAAAGAATACGAAGACAAAATTATGGAATTAATGAATGCTGTTGACACATGAATTCAAACTCCAGTTAAAGAATTCGACAAACCATTCTTAATGGCTGTTGAAGACGTTTTCACAATCACAGGTCGTGGTACAGTTGCTACAGGACGTGTTGAACGTGGAAGATTAAACCTTAACGAAGAAGTTGAAATTGTTGGTTTACACCCAACTAAGAAAACTGTTGTTACAGGTATGGAAATGTTCCGTAAAAACCTTAAAGAAGTTCAAGCTGGTGACAACGCTGGTTTATTACTTCGTGGTATTGAAAGAGCAGGAATCGAACGTGGACAAGTTCTTGCTAAACCAGGAACAATCATTCCTCACACAGAATTTACAGCAGCAATTTACGTATTAACCAAAGATGAAGGTGGACGTCACACACCATTCTTCAAAAACTATAAACCACAATTCTACTTCCGTACAACAGACGTTACAGGTGGTGTTGAATTTGAAAAAGGCCGTGAAATGGTTACACCAGGGGAAAATGTTAACTTAACAGTTAAACTTATCTCACCTATTGCCGTTGAAAACGGAACTAAATTCTCAATTCGTGAAGGTGGACGTACAGTTGGTTATGGTAATGTAACAAAAATTATTAAATAATTTAATTACAATACACATAATAATATTTTGAAATTGCAGGGTAGTTTACCCTGCTTTTTTATATATCTAATCAAATCTTTTTGAAACTTTTAATATATAATTAAAAATAATATTTAAGGTACATATGAAAAATTTAAAAACTTTTATAACTAGAGATGTTAAAGTAGTTAAAAAAGTAGCTCAATATGTTTTAGATCATTTAACTAAAAGCAAATTAGTTTTGATGAATGGCGACTTAGGAGCTGGTAAAACAACTTTAACTAAAGAAATAGCTAAATTATTAAGCATTGATGAAGTTATTACTTCACCAACTTTTAACTATATGAAAGTGTATGATGGTCTTGTTCATATCGATGCTTATAATCTTAAAGGCGATATTAGTGAATTTGAAGATTACTTTGAAGATAATGTTGTTGTTATTGAATGGGCTAATAGAATCAAGCATTATTATAAAAACTATTTAGATATCAATATTACTTTAGATAAAGACAACAATCATGTATTTACTATTAAGGAGGCTAAATAATGAAGTTATATTTAGATACAGCCAATGAAGACTTTGTTTTAGCTTTGCTTAATGATAAACATCAAGTAATAGCTCAAAAAGTTTTAGAATCATATCCTAAAAAAGTCGAATTGATACCCCAATTAACAGAAGAAATATTAAAAGAAAATAATTTTAAAATTCAAGATTTTGATACTTTTTATATTAATATAGGACCTGGTTTTTTTACAGGGGTTAGAATTGCTTTAGTTTATTTAAGAACTATAGTTTTAATTACAAAAGCTAATATTAAAACTATTAGTACTATGCAAATATTAGCTAAACAAAACAGAAGAAAAAGAAGTTTTAAAATTAATGCTCAAGGCAATAAATACTATTTATATGAAACTAATAAAAATAGTGCATTTAATTATAAAAATATTAAAGTTGAAACAGGTACTTTAAAAGCCTATGACAAAGTTAATTATTTTGACTTTTTAAGCTGTTTTATAGATTATTTGGACGAATTCAAAAGCTATAAAGATGTAATGGATATTGAACCTTATTACATTAAAATGCCACAGATAGGAGCTAAAAAATAATGCGTATTTTAGGAATAGAAACAAGCCATGATGATACTAGTATAGCCCTCTTAGAAGATGGTAAAGTGTTAGCTTTAAAAACTATTAGTCAAATAGATATTTTCAAAGAATTTGGTGGCACAATTCCTGAAATATCTTCAAGAGAACATGTTAAAAATATTAACTTAATTGAAAAAATATTTTTACAAGAATTTGATCTTAATACTATTGACTACATTGCTTATACTCAAAAACCTGGATTAATCGGAACCTTGCAAATAGGTTTCTTATTTGCTAGTGCTTTAGCTCTTGCTTTAAATAAACCTCTTTTACCTATTAATCACCTTGAAGGTCACTTTTACTCAGCAGCTATTAAACAAGAAATAAAATATCCTGCATTAAGCTTATTAGTATCAGGTGGTCATACTCAATTGATGTTAGTTAAAAGTCCTTTTGACATTCAAATAATTGGACAAACACTAGATGATGCTGTTGGTGAAGCTTATGATAAAGTAGCTTCAAGATTAGGTTTTGGTTTTCCTGGTGGTCCTATTATTGACAAAATGTATCAAGATTACAAAGGCGACTTTGTAGCTTTAACCTTGCCTCATACTGATGGATTATATGATTTTAGTTTCAGTGGTCTTAAATCACAAGTTTTAAATCATTATAATAATGCTTTGCAAAGAAAGAAAGCTTTAAATAAAGAACAACTTGCTGTTAGTTTTCAAGAAACAGCAGTTACATATTTAATTAACAAAACTAAATTAGCATTAGATAATCATCCAGAAATTAAAACTTTAGTTTTAGCTGGAGGAGTTAGTGCCAATAGTGAGCTTAGAAAAAGATTTTTAAAATTAAGCACTAAAGCTATAGTGCCTAATTTAAAATATTCAACTGATAATGGTGCAATGATTGCTATGTGTGCTTATTGTAAGATTAAATGAAATTAATGATTAATAAACATTTTTTTGCATCATCTAAACTTTTACAGTCTCTTAATCCTTTTTCTGCCCCCCCTTTTTTTTAATTAAATCAAGAGAGACGGGAGAGGGATTAAGATTTCTTGCATAGCAATAACTTTTCCTATTTTAAAATTCAAATTTTAAAAATAAGATAATGTTTTTTAGGCATTATCTTATTTTGTTTCACAATATTATTAGGTGTTGATGTAAAGTTAATTCCTTTCTATTAAGAAATAGAGAGACTTTACACAAAATTCTAAACATTCTCTAATTTAACTCCATTACCATCATTTACTTCATTAAATTCTTTTGCGAAATTTTCAGCACTAAAAGTAGTGGTGTCATTGTTGTACTTTTCATTTATAAATTTTATAAATGCATCATATATTTTTTCAATATGTTCATCAAGGGTTAATGGAGAGATTGAATGGGCCACAGCTAGAATTTTATATTTTATTTTTAATTTGTAAAGTATTTGTAAATCTTTATTTATTTGTTTTACTATATTTCTTTTAATATTTTTTATTTTTTTAATATCGGTTTCTTGATTTTGTTCAAAGTACTTAAAGAAATTATGTGCTACAGCAGGACTATCTTTAATTTCAAAAATATTTTTATTTTTAAATACAAAAAAGTCTTTAATACCAGGTCAAATATCATTAATATTAATTATTTTTTCAAAATCAGCAGCATGCATTTTGGTATTTCCAAAACTTATTTTATATTTGTTATTATTATCTACACTTAGAGTTGCATCTTCAAAATAACGCGGTTCTTTAAAGATAAGTTTTGGCAATTCTTTTTTAATTTTTTCATCTATAAAATCTTTTAATTTTATATAAGGCCAAGTATTGCTTTTAACACTTTCATCTTTAACATAATTAATTATAGAATCTTTGATTTTTTTAATGTCATTATATAAATAATATTCTTTTCATTGATTTTTTCATGAATCAGTTGTTTTCATACGTTCTGAGTATAAAAGTATTTCATTTTTTCTTTTTTCAATATAATCGTTGATTGCTAAGTCAAGCAACGTTTCACATTCATTATCTACATTATCAAAAATAATTTCTGATTTCTTTGTGATTGCATATAACACATTGTTGTAATTATTGTTTTCAATTTCGTCCCAATTGAAATATTTTTTTGAAAAATGTCTAAATTTCTTGATTTTTAAAGTAATTTGTGGTTTATTATTTTGATTTGAAACTTCAAATTCAAAGTTTTCGTTTGATAAAGTAGATTCAAATATTGATTTATTCAAGAATTCATTATCAAACAAGAAATTATATATTTTGTCTAAATTTTTTATTATATCTTCGTTAAATAGTAAATGAGAAATACTTTCTTCACTTCATTCCGAATTTTCAAACATTATAAATTTAGAGTTTTTTACATTTTTAATGACATCAAGACTATTAACATCAAATATGAATTGATTGCCACCTTCAAGTAAATTTTTTATTATTTCATATAATGATTTTTTGCCTTCATTAAAATCCTTGAATTCATCTTTAAAAATTCTTTTTAATCTGTTAATCTCTTTTTCGTTTTTGTCTGCAAATAATAATTCATCTTGTAAAATTTGTCTTATTAATCATTGTCATTTAGTAATTTTTTCATCATATTTTAATTCTTTAATTCCAGTTTTTTCACCATTAGAACGAATTTTTCTTTCATTTAAAGAAATCAAGAAAAATCTTTCAAATTCTTTTTCATGAGTATTTGGATCTTCTGCTCCTGTATCATTTAATTTAATATCTTCAACTCTTATAATTTTTTCATCATTTTTTTCATAAGTTGATATATTAGTTTCACTTGATAGATTTGGTACTGTATTACTAATATTTTTAGCCAAATGATTTTTCTTAATCACGTTTAACACATAATAATTTTCTAAATTGTCTATTTTGCTTTGTAATTGGGAAACAAATACATTTCAATCTCCGGCACATTCAAAATAGTTAATACTTTCATATTGATTGTTAACACTATTAAAATATTGGAATTCTTTATATTTTTTATTCACTATTCGTTTATTACCCGAATTATTATTGTAATAATCAATTAAATTTGAAAGTTCAAGCTGAGTGTCTTCATTAGCAATTTGTGTATCGCAAATATATTTTACAAATTTTTCAGAAACTTCATTTGTTGAATTAATTTCGAATAATCCAAAATGTTTGTTTTCATAATTTGATTTGAAAAATAAAGTTAATAATTTTTTTAAGTTTTGAACATCAGTGAACTTAAATTCCTTTGTGTTATTGTCATCGATTTTTAATTTTAAACTTTTTTCCTTTATACTTCATTGAACACTAGGGAAGCTTTGTTTATTAAGAATTATTTTAAAGTTTTGAATTAAATTGTAATCTTTAAATAAATTTTCTAAATCTTTATCAAAGTTCTTTTGTTGATTACCATTAAATAGATTTCTTGCATTAATCAAAGTATATAAATTGTTTTTTATAGTTTTTCATTCGTTTATAGATAGTTGATTACCATCATAAGATATGTCACCTATAGTAGAATCTCCTGTTAAAAATTCTAGCGATTTAACAATTTTTACTTTTTTTCCTCGATAATTTTCATTTGCAAAGATATCACTTAAAGAGATGTTAGCTGTTTTTGAACTATTTGCTATTTTTTCTTTAATTCTGTTGTAAATTTCAATTATAATTTCGTCTTTATTTGTTGCTGTAACTCATTTATCATATTTATATTCTAAAAATCAAATCGAACTATTTAAATAATTTTTTAATTCGTTATATTTTTTAAAACTATCAAAGTCAGAAACACCTGATGGTTTTAATGTTTTTGATTCAAGAGTTACAATTTCTATATTTTTATCTTTGTATTCTGAAGGATAATTTACACCAGCTTTTATATTCAATTTTGATAAATCACATTCATTATCAATTTCAGAAATATAAGCATTAGTCAATTTACTTTGAATATCATTTTTTATTTTTTCAATTATTTCTTTTCTACATTTTTCATAATCTAAATCTTGTAAAGGATATTTAAAGATATTATCTTTTTCTTTAAATAATTCATCAACTATTTTGTTGATATTGTTTGTAGCAAAAAAACCTATTGTATTAAATTCATAAATTTTGTTTACAATATCATATGTTACGCTTATTTCTTTATTTAAATTATTAATGTCGAGATATGTTGTATTATCTTTTTTAAAACCAATATTTTTTAATGAAATATTAAATGTATATTTATCATTTATAAATGAATCAAAAAAATTTGAATTAGTACCGTAAAAGTAATTTGTTATTTTATCGAATTTCTTATCAACTGATAATTTTGCAACTTCTGAATCATAATAAAATGTTTTTTTGTTGTAATCCGCATTTGAAAGTATATTTTCAAAACGACTTATGAATTCTAATTTTGAATTTGAAATGTATTTTTTAATAAAAGTTTGAAATACAATATTTATATTTTTTTCATCTTTTATTCCACAATATTCCTTAACTATATTTAAAGTATTGGCATTGTTTAAATCCGGCATTATGATAAAGTAATTATCGGTCAATTTGAATAATTTTGCAGAAATTCAACTTGTAATAACATTGTTATTTTGATTAGTTATAACACTTAAAGGATCTATGTTATTAAATTCATATTTTCATATAGGCATTTTAAGTTGATTTTTTATTTTATTTCAATTAGTTGCTTTATTTGAATCTACAAAGAATAGACCAAAACTATTTAGGTTACAAATAAAATCACTAAAATAATTATCATCTTTTTGAAGTTTATTTGTTAAGTATCCTAGTTTGTCATAATAATTTAATTTAAATTCTTAATTATTTTTTTTAAATATTTCTTTTGTAAATGGTGTTTGACCATTAAAGTTAATACTTAAAAATTTTGCAAAAGACAATCTAATAGTTTTTGTTGGAATATCTACAATGTAATCAACAATTTTGTAATTTACATTATCTAAATTTGAAGTGTAATATCCAACATTACTGGTTTGTCCTTCGTATTGTGATTTTATAATATTTTTTATATCTGAATATAAAGCATTATTTGCAAATAGTAGATCATTTAATTGAGCAATTGCATTTGAACGACCACCATTGTTTGCTCATTCTGTTAACTGTTTAACAGTAATATCAACTCTGTTTTTAATTCTGATTTCTTGATTGTTTTTTCCATTAATCCCTAATTCATAGATACTTTTTTCATTATCTCTTCCATAAAAATTAATAACTATTTTTTCATATTTTGTATTGTTGTCTTTTGGATTTGAAAAGCTTAAATTAGCATTATATTTTTTAATGTATTCTTTTAATTCATCATTTTTATAAAATGTAATGTAATTTTTAACACTATCTTCAAATCATTCCTTACTTTTATCAATTTCTAAAGCCATTGCTAATTTTTTAAGATCCTCATCATCAAGTGTTCATTCATTATCAACAATTTTTCTTTTGACTTTAAATGTTTTATCAACTACTTTAATTTCAAAGTTATATAAATTAGTGTTTGTGTATAAGCGGATTTCGCCTTCTTTTGAAGACTTTCAATATTCTTTAAACAAATTATAAACAAGGTCATTTTTATCTACTTTTTCAGGTACTTTGTATAAAGTTTTTTGGTCTTCAGAAATGAAATATAAGTTAATCAATGGTGTATTTGGATTAGAAGAAATTAGTTTAGTATTATATTTTTTATTTTCTGAGTAAGATTCAATAAACTCATCACAACCTGGTTCACCTTTAATAGTACTAAATTTTTCAGGAGGTAATAATTCATTTTTAATATTATTTGAAAATATTTTTCTATTGAAAATGTCATCTAATTTATTATTCTTTAATAATGACTCAACTTCTTCGGCATGTTCTTTATTAGTTTTATCGATTGAAATATCAAAAATCTTAATTCTTGGAGAAATATCATATCTAATTTTAAAAGTATTGTTGGTATTTTTTAATTCAGCTTTATTTGATTCATTGCCAAAAACTGTCGAATTATTAAAATCAGAATTTTTTAATAGTTCACTACAATAAAATGGATAATCAATGTTTGTTTTTTTATTGTTTTCCATTTTAAATGTTTCTTCTTTAATAAAACCTTTAGAAATAAATAATTGTTTTAAATTAATTTCTCAAATGTCATTTTTTATTTTTTCAACTATTGATTTATCATTTGAACTTATATAAATGTTGTTTATTTTTAAAATAACCACATCATATTCTGAATTGCATTGACCTTTAACAAGCTCAATTCTAGGCATCTTTTCTCCAAAGTTTCTTAGTTTAGCTTTGAATCTAGTTTCAATTTCATTTTTTATTGTTGAAAAATTAATTTTTGACTCATCGCTTTCATTTTCTCAATCAATCATTCCGCTTCTAAAGTAAAAATGCAAGTCAAAGTTAACTTGTCTACTTTCTCTAGGCATAAAGTTCAACATAATAGTTTTATTACCTGCAAACTTATCGCTATAGGTAATAAATTTGTTGTTAATTTTTGCACCATATTCATCTATTCTTCAGTCTTCATTTTGGCAAATTTCATTAATAAATTTGTTGTCTTTAAGTAAAGACAATACTTGTTCTCAAGTGCTTCCTGATTTAACTTTTATTGAATCAATGAACACATCTTCATTATTTTTGTTTCTATATTTTAGAGTTAGTTTTGTTCAGCTATTTACATTGTAAAAAGCAGCAGCACTAACTACTCCAAGTGTTGTTAACGAGAGTGCAGTTGCCACTGTCGCTATAACTTTTTTTCTTTTGTTTTTAATCATAATTTCTCCTAAAAATAATTCATATATAAATTATATACTATAATTTTTTCGTTTTTATTAGATATTATTAAGTATTAAATTTGAAAAATATTTATATTTTTCTAATTCAATTGTTTTATAGTTGTTTTTATTTAAAAAATATTTAAAAATATATAGAAAAAGATTTTTTAATGATTTTGTATTAAAAAAACTAAAATTTGAAGCCATTTTTTTACTAAAAAATCTGTTTTATAGCCTTTTTGGTTAAAAATAAAAACAACCAAATATTTGTCGCAAAAATAAATAATTTATATTAATATATATTTTAATATAATATAATCATATTTAATATATTAAATGGAGGAATAAAACATGGCATACAAAAAACCAGAAATTACAAACAAGATTGTTCAACATGTTGGTGAAATTTCAACAACAAATAGTGGTTATAAAAAAGAACTCAATTTAGTTTCATGAAACGAAGGTGAGGCTAAATATGATATTCGTGACTGAAGTGAAGATCACTTACGTTCAAGCAAGGGCATTACTCTTAGTTTAGAAGAATTAAAACAATTAAAAGTTTTGATTGACAAAGAGCTAGTAAGAATTAAATAATTATGATTTTAGCAACTAAAGATGATAAAGAAGTAATTCTTAAATTCTTAAATCAAGAGCCAATTTATAATTTACTAATTGTTGGCGACATTATGACTTATGGGGTTAGAACACCTTATCTTTTTACTTATATCATTAAAGAAGAAGAGCAAATTAAAGCAGTAATATTGATATTCAATAAAACAGTTTTAATTTATGATCCTGAATTTAAAGTAAGTCTAAAGGATTTAAAATCTTTAATTAAAAAACATTCACTTATTAATGTTAATTTAAGCGAGAAAATGTATCAACATTATCAAGATGAGTTTGAAAATAATGGAGATAAATACAAGGTTCATCGTCAAACTATAGCAATATTGGAAAATAGATACACTGGTGATACTTCACTAGCTAAAAAAGCTATTTTTACAGATTCAGAGCCAATAGTTCGTAGTAGATTTTTAATTGATGAATTTGTAGACTTTAGAGGGACTTATCAACAAGAAGTAAAATCTTATAAAAATGCTTTAATAAAAAAAGTTTCAACGCCTTTCATTATTAAAGACAAACAAAATCGAGTAGCATCACATGCTAATATATTGATATCTTCAGATAAAGCATCAATGATTGGCGGTGTGTTTACTTTAAAAGAATATCGACAAAAAGGTTATGCAATACAAGTAGTTGGCGCCTTATGCAACCATATTTTTGCCAACAAAAGAGTACCGATGCTTTTCTTTGATAACCCAGCAGCAGCACAACTATATCACAAAATAGGTTTTAAAGATTTTGGCAAGGTTTTTACAATTAAAATAAATTAATAAAGGAGATAATATGCTTAATTTTTTAGAAAACAGAATTCAAAAGTCAATTCAAAAAATGGCTAGAAAAACTGTAGTTAATGAAGAAGACATTTTAGAAGTAACAAGAGACATAAAAATGGCTCTTTTAGAAGCTGACGTTAACTTAAAAATAGTTAAAGAATTTATTGTTAATGTTAAACAAAAAGCTTTAGAAAGCAAATTAGTTGGTTCACTTAATGCTAGTCAACAAATGATTAAAATTGTTCACAATGAACTTGTGGACATTTTAGGTGGCAAAGTCCAAGAAGTTAAAATTACGGCTCGTCCTTATATTATTATGATGGCAGGACTTCAAGGATCTGGTAAAACTACAGCTTGCGCTAAATTAGCTTATTACTTAAGAAAGAAAAACTTTGTAACAAAACCTTTAGTAGTAGCAGCTGATATTTATCGTCCAGCTGCTGTTCAACAATTAGTAACTTTAGCTAAAAGTATTCAAATTGATTACTTTGAAAAAGGCACTAATGTTAGTGCTCAAGATATTGTCAAAGAAGCATTGAAAGTCGCTGATGCACAAAAAAATGATTTAATAATCATCGATACTGCTGGTCGTTTGTCAATTGATGAGCCTCTTATGCAAGAACTTTGAGACTTAAAGAAAATTGCTAATCCAAGTGAAATATTCTTTGTAGCTGATGCTTTAAGTGGACAAGATATTTTAAATGTTGCTGAAACTTTTAATAATAAATTGAAACTTACAGGTACTATTATTACTAAATTAGATTCAGATGCTCGTGGTGGAGCTGCATTAAGCTTAACCAAGATGTTAAAATTACCAATTAGATTTATTGGTACTGGTGAAAAAGTTTCAAACTTAGATCTTTTCTACCCTGATAGAATGGCTGATAGAATTTTAGGTATGGGTGATGTTATGAGCTTGATTGAAAAAGCTGAAGAAGTCATCGATCCTAAAATGGCTAACAATATGGTTGCTAAAATTTTAAGAGGTAATTTTACTCTAGATGATTTAATGGAACAATTAACTCAAATTAAAAAACTTGGCAAAATGAGCAAGATATTAAAAATGATTCCAGGACTTGCAAGAAAGATTGATGAAGATAAAATTGCTGAAGCAGAAATGAAAATGTATAACTATGAAATTTTGATTTCATCAATGACACCACAAGAAAGAAAAAATCCTAAATTACTTAAAAATGCTAATCGTAAAGCTAGAATTATTAAAGGTAGTGGACGCAGTGCTCAAGAATATAATCGTTTACTAAATGATTTTGATCAGTTAGTTAAAAACATGGGTGAAATGGCTAAAAAAATTAATGCAGGTAATTTTAGCGATATTGCTAAAATGGGAGGTTTTGGCGGAGGTGCGGCTTAATGCATAAAACTGTTGACCATCACCACCCTAAAGGTTTCTTTTCCTATAGTGGAAATCATTTGAATTTCACAGGCTTTTCACAGGCATTCTTTTATATAATAGCTTTTTTGTCTATAACTTGTCTTTTACTAATTTGACTGTTTCATAGACAAATTAAACAAAAGTACTTAGTTAAAATTAAATTATTTTATTTACAAAAAAGAACATTTTGACTATTATTTGGCTTGATTATTTTATTAGGAATGGTAATTCATATTATTATATTAGCTGCTGATAAATTTCATCGAGCTTGAGAATATTTGCCTTTTCATTTTTGCCGAATTTTGATGTTATTAATTGCTCTTTCATTAATATTCAATAAACTCCATTATGTTAAATATTATGGCTTTTTAGCAATTATTTCTGGTTTATTGGCTTTAGTTAAAGTTGATTTTAACTTTACGCAAGAAGAAGGCAAGGATATAATATTTCCAATTGGAATTGATAATTGATATTATTGAGATTACTTGTTTGCACATGTTTTTGTATTATTGATGCCCGCAGTAATGTATGCTCTCAGCAATAACAAAATTAGATTTAAAGATTCAATTTTTACAGTTATTTTCTTTTCAACACTTTCATTAACAATGTTTTTAATTAACTGAATAACTTATACATATAGTAAAAAATTAACTTGAAAAACAATGAATTACTTTTATTTAGGGCCTAATGAATATAATGGTTTTAGAGACTTTATGGGACCGCTTTCAAAATGACCTTACAGTCTTTTTACTTATATATTTCTCGGCATTTTAGCAGTTATTATCTCAACAATTTTTTACTGTTTTCAAGACAAAATTCATTTGGCTAAAGTTGAAAATAAACGAGTTTTAAAATGAATCAAAAGTGAAAATTGAAAAATATATCGCTCTTCATTTAATACAAGAAACCAAAAAAATGATGAATTAAATTCTAGTTCTAAAGACTTAGAAAACTGTCAAGATAATAATGATTCTATTGAAAATAATAAAGAAAATATTAGTCAAACAGTTGACTAAAAATTAATTAAAAAAGAATCAATTTTGAGCTCAAAAAAATCTCTTAAAAAAATTGATTCTTAAAAAATGAATGTAATATAATACTATTTATGTTAGTAGTTTATGACATGCTTTTTGTGTACTTTTAAAGAAAAATACACTGGAGATCTTATGGATAACCAAAAAGAAAAAAGAGTGCATATAATCTTTTGAAGTTTAACTTTGATTTTAGGTTTATTATCACTTGCTTTTTTTAATTATAAAAAAACTAAATTTGCTGTAATGCTTGGCTATTGAATTGGCACAAGTTGTGGCTTTTTAGCATACGAACTAAGAGAGTTATTATCTTTTAAAATTATAGAAACTAGTAAAAATAGAGCAATTATGCTTGGTTTCTTTGGAACAATATTAAATATGCTTTGAATTGGATTATTGCTTTTTGCAATTTTCAAAATTAATTCAAATGCGCTTAATAGTCCTTACACAAAAACCATTGAAAAAAATAGTTTTTGACCAATAAACATCATTGCTTTTATTCCTGGTTTTATGACATTCTATTTTTCTCTTTTCATTGGATATTTAAGTTCTGAAAAAGACAAAAAAGTTCAAAATAACTAGAAAAGGAGGTCAAGTGGAAAAACTGACACAAAACTTGTGAAAATGAAATATGCCCCAAATGTTTTCCTTATTTATTTTGATATTAGTTATCTTTATTATTTCACTAACCACATTTATCTTAGTAAAGAAAACATCACGTCTAGATAAAGCGCCCATTGGATTCTTGATAGTTATGGAAAGTTATGTGACATTCTTGGATTCAAGTTTTGAAGAAAACACTGAAGGTGCTTTACCAAAAGCAAAATTTTACATTTTTGCATTGTTTACATTTTTATTGCTAGGTAATTTGTTAGGTCTTTTGGGCCTTAAACCAATTGCAACTAGTTATTCAATAACATTTGCTTTAGCATTCATTTCATTTGCTGGAATTTATGTTGTTGGACTAATTTATCAAAAATTCAGATTCTTTAGTAGATTCTTAAGTCCAATTGAACTTGTAGGTCAATTTGCACCTTTAATTTCACTTGGCTTTCGGATTTTTGGTAACATCACTGGTGGTGGAGTTATTGTGTTCTTACTTTATAGTTTCACAGGATGAATCTGAACACAAATTCCTGGCCTACTAGGTGAATGATTCTTTATTGGATCATTAATTACACCATTTTTACATATGTATTTTGATATGTTTGGTGCAATAATTCAAGCTTTAGTTTTCTGTACATTAACAACAGTTTACTGATCAAAAGAAGCTGTAACAAATGAAAACAAGAAACAACAAGATGCTCAAGAAAAAGCAGAATCTGAAAAATCAGTTGCTAATGTTGAAAACAAAGCTGTTGCTTGATCAAATTCAAGCAGCGTATATTAAAAATTAGGAGGTATTTATTATGACTCAAGGTGGAGGTTTAGTTGCAATCGGTATTGGTATTGCAATGTTAGGTAGTTTTGGAACAGGTTTAGGACAAGGCTTAGCTGCTGGTAAAGCTACTGAAGCTGTTGGACGTAATCCTGAAGCTGCAGCTAAAATTCGTTCAATGTTACTTATTGGACAAGGTGTTGCAGAATCTTCAGCTATCTATTGTTTAGTTGTTGCCTTTATTCTAGCTTTTGCTTTTACAAAATAATTTAATAATGCACACAATCAATAAATCTTTAGGAACAAAAATTGAGGAAGAATTAAACGGTTTATTTCCTAGTGTTCCGATGCTTGTGGCAACTTTAGTTGCTCTTGCAATTATTATTTTGATTCTTTTCTTCTTATTATATAAACCAATTAAAAAGTCTATAAAAGCAAGACAAGATTATATTCAAAGTAATATTGATCAAGCTAAAGCAACTAATGACTTGTCAAAAAACAAGTTAAAAGAAGCAAATGACAAATTGATTCTAGCTCATGAAGAAGCTAATAACTTAGTTAAAAACGCTAAAATTAGAGCAGAAAAAGTTATTATTTCTTATACAGCAAAAGCTAAGATGGAATCAAAAAGAATAATAGAAGAAGCAGAATTAGATATTGAACAACAAAAAGATTCTTTAATGGAAGATTCAAAAAATCAAATTGCCAAAGCAGCTACAGAATTGTCAAGACAAATACTCAAAAAAGAAGTTTCAAAGAAAACTGAAAGTGAAATTATTGACAAGTTTCTTGAATCTTAATTTTTCGTATTTATATATAAAACAATTAATTTAGTAAAAATATGTACTCAAAAGCTAATCCAGAAGGATATGCTTTGGCTCTTTTTGAATTGACAAAAGAAGAAAAAGATATGAAAGCTATTAATGAAGTTGTTGTCTCTCTTTATAACACAGTTGTAGAAAGTGATAAACTCGTTATGATATTAAATGATCAAGCTTTAGAAAAAAATAAAAAGTTTGATTACATTGACAAAATTTTTAAAAACATTTCAAGTAGTAAACTAATAGTTAACTTTTTAAAAGTTTTAGTAGAACGCAATGCAGCTACTCTTTTAAGAAGATCTCTTGAACATTATTTAAAGCTCTCAAATGAATTTTTAAACATTCGATTTGCTTTAATTACAAGTGCTTTTGAAATTAGTGAAGACAAACTTAAAAAAATTAAACAAAAACTTGAAAAGAAATATAACACTGAAATAATTCTTAAACATCAGATAGATGAAAGCTTAATTTCAGGTTTCAAAATTAAAATGGATAGTTTAGTTATTGAGCAAAACTTTAAAAAAGATTTACAAGAACTAAATACTATTCTTACTAAAAAAGGAGGTTTAGATGGCTAGTAGAACAGACGATATTAGTGCAATAATTAAAGATCGAATTCGCAATTTCGACTCTAAAGTTGACTACTCAGAAGTCGGAACTATTATTACTATTGGTGATGGTATCGCCTTAGTTAAAGGTCTTGACAATGCTAAAAACGGTGAAATTATTTTATTCAAAAATAATATCTATGGGTTAGTTTTAAACCTTGAAGAAGAAGTAGTTGGAGTTACCATTTTTGGTAATGCTAACTCACTTTCAGAAGGTGACACTTGCAAAAGAAGTGGTGAAGTTATCTCAATTCCAGTTGGTGACAAACTTCTTGGCCGTGTAGTTAATGCCTTAGGTCAACCAATTGATGGCAAAGGCAAAATTAATACTACTGTTAAAAAAGAAATCTTTAAAGTTGCTCCTGGTGTTATGACACGTCAAGAAGTTAACCAACCTCTTGAAACTGGTATTATTGCAATTGACTCAATGATTCCTATTGGCAAAGGTCAACGTGAATTAATCATTGGTGACCGTCAAACTGGTAAAACTGCTATTGCAATTGATACAATCATTAACCAAAAAGGAAAAAATGTTAACTGTATTTATGTGGCTATTGGACAAAAAAATTCAACAGTTGCTCAAATAGTTAAAAAACTTGAAGACACTGGCGCTATGGAATATACAACTGTTGTAGTTTCTGGAGCTAGTGAACTTGCGCCTCAACAATATATTGCACCTTATTCAGGCGTTACTATTGCTGAAGAATGAATGACCAAAGGTAAAGACTGCTTAATTGTTTATGATGACTTATCTAAACATGCTATAGCTTATAGAACACTTTCGCTTCTTCTTCGTCGTCCACCTGGACGTGAAGCCTACCCCGGGGATGTTTTCTATCTTCACTCTCAACTTTTAGAAAGAGCTGCTAGAGTTACAAAAGAATATGGTGGAGGTTCAATTACTGCTTTACCTATTATTGAAACTCAACAAGGTGATATTTCAGCTTATATTCCAACTAATGTTATTTCTATAACAGATGGTCAAATTTTTACTAAAGAAAACTTATTTAATTCAGGCCAAAGACCTGCTGTTGATGTTGGTTTTAGTGTTAGCCGTGTTGGTAGTGCTGCTCAAATTAAAGCTATGAAAAAAGTAGCTGGTTCACTTAAACTAGAATTAGCTCAATATAATGAAATGTTAGCCTTTGCTCAATTTGGATCTGATCTTGATGATTCAACTAAAGCAATCTTGCAACATGGTGCTAAAGTTTATGAAATCTTAAAGCAAGAACAATATGCACCTATTAGCCAAATTGCTCAAGTAGCTATTTTGCTCGGGGTTAAAGAAAGAATAATAAATCCTCTTCCAGCTAAATACATAGCTCTTTATAGAGAAGAAGTAATTAAATACATCGAAAGTGATCCTGAAGGTGTTTCAATTGCTCTAGAAATTCATAACAATAATAATGAATTTAGTGATGTTCATTTTGAAACAATAACCAAAGCAATTTTAAAAATAGTAAAAAGAATTATTGCTTCAATTCCTAATTATGATCCAAGTGATTATTTGCCAATGCCTGAAAAATACAGTGAAAAGAGTAAAAAATAATGGCTGGTACTCAAGCAATTAAAAGTAGAATTTCTACTGTTGAATCTATTCGCAAAATAACTCATGCTATGGAACTAGTTGCTGCTAGCAAACTTAGAAGAGCAAGAATTGAATTCGAAAATGTTAAAGCTTATAATGAAGTAATTAAAGAAACTTTTCGTTCAATTTTAAATCATTTAAGCCCAACAGAAATTAAACATTTGTTTCCAATTCATAACATTGAAAGCAGAATGTTTATCATTGTAACTAGCGATTTAGGATTAGCTGGAAGCTATAATTCAAACATTATAAAATTTGCTAAAAACACTATAAAAAAGACAGATAAGGTAATTTTAATTGGCCGTAAAGCTATTAGTTCCTTAGCTTCTTTATATGAAAATCAAATTGTCACACAATTTAACTGTGATAGCAGTGAAAAAGACTACAACATCGTTCACGACATTATGACAATTGTATTTGAACTTTACAATCGAAAACAAGTAAATAGTATTAATGTAATTTATAACAAATACATGAATAACCTTGTTCAACAAGAAACTTGCGATCAAGTTTTTCCTTTTAAATTTGATGAAGATAAAAAACAAAAACACGCTGAAAAACACAAGGAATCACTTAATTCAATTATCGAATTTGAACCAAGTGCACAACAAGTTTTAAGCGAATCAATCCCACTTTTTATTGACGCTGAATTATATTTAGCTTACACTTCAGCCAAACTCAGTGAAATGGCTGCAAGAAGAAGTGCTATGGAATCAGCAACTGATAATGCTGATGAATTAATTGACAACTTAAATCTTGAATTCAACAGAAAACGTCAATCATCTATTACTCAAGAATTAAATGAAATAGTTGCTGGAGCTGACGCAGTATAGGAGGTAAAAATGGCAGTAAAGAAAACTACAACTAAAAAAGTTACTAGTGCTAAAAAATCAACTACTAAAGTCACAATAACTAAAAAACTAACTGAAAAAATTAATAAAAACAATGTTGGTAAAATAATCCAAGTTTTAGGACCTGTAGTCGATGTTAAATTTAACAAAGGCTTTTTACCTTCTTTATTAAATGCACTTAAAGTTTATTCAAATGATGAAGTTTATACTTTAGAAGTAGCTCAACATATCGGCGATGATACTGTTAGAACTATTTCAATGGTTTCAACTAATGGACTTGCAAGAGGACTTGATGTTATTGACACAGGAGCTCCCATTAGTGTTCCTGTTGGAGATAAAGTTTTAGGTCGTATGTTTGACGTTTTAGGTCAACCTATTGACTTACAAGAAGCTCCTAAAGCTCCACTTATGCCAATTCATGCATCTGCTCCTAGCTATGAAGAACAAAAAACAGTTAGTGAAGTGTTAGAAACTGGTATTAAAGTTATTGACCTTTTAATTCCATATGCAAAAGGTGGAAAGATCGGACTTTTTGGTGGTGCTGGTGTTGGAAAAACAGTTTTAGTTCAAGAATTAATTAATAATATTGCTACAGAACATGGTGGACTTTCAGTTTTCGCTGGTGTTGGTGAAAGAACTCGTGAAGGTAATGACCTTTACCATGAAATGAAAGCAGCTGGTGTTTTAGATAAAACTGCATTAGTCTTTGGACAAATGAACGAACCTCCTGGAGCACGTATGCGTGTTGCTTTAACTGGTTTAACAATGGCTGAATATTTTAGAGATAAAGAAAACCAAGATGTGCTTTTATTTATTGACAACATATTCCGTTTCACTCAAGCTGGTAGTGAAGTTTCAGCCCTTTTAGGTCGTATGCCTTCAGCTGTTGGTTACCAACCAACACTTGCTACTGAAATGGGTCAACTACAAGAAAGAATTACTTCAACACGTCGTGGATCAATTACTTCAGTGCAAGCGGTTTATGTTCCTGCCGATGACTTAACTGACCCTGCTCCTGCAACAACATTTAGCCACCTTGATGCTAAAACTGTTTTGGATAGAAATATTGCTGCTTTAGGTATTTATCCAGCAGTTGATCCTCTTGAATCAAACTCAAGATTATTAGATCCTCTTGTAGTTGGTCAAGAACACTATGAAGTAGCTCATGGAGTTGTTAACATTCTTCAAAGATTTAAAGAACTTCAAGACATTATTGCAATTCTTGGTATGGGTGAATTAAGTGAAGAAGATAAATTACTTGTTGCTCGTGCTCGTAGAATTAGAAATTTCCTTTCGCAACCATTTAATGTTGCTGAAAAATTTTCAGGTAAAAAAGGTAAATACGTTCCTATATCAGATACTATTAGAAGCTTCAAAGATATTCTCGAAGGTAAATACGATGATTGCCCTGAAGAATTCTTCTTATATGTTGGCTCAATCGATGAAGTTGAAGCTAAATACAAAGAATATTTAGCTAAAAAAGAAGCAAATAAAAATTAATAAAATTTGCTAAAACAACTGTTTCATAGTTATATAAGAAAAAATGTTGAATAAAAAAACAACACATTTAAAGATAACAACATTGGAAGAAATCTTTTTCGAAGGTGATGTTCGAAGTGTAACTCTTAGAACTAAAACTGGTGGTGCAATTTGCTTGCAACCAAGCCGTACTCCTTTCTTTAGTACAATAGATATTTGCGAATTAACAATTAATAGTCCTAAAGACAATGACTTTAGAGTTTGTTCAATTGGCGGTGGCTTAGTTTATGCTGATGCAACCAACATTAATATCATTACTGATGACATTATTTTTGGTAAAAATATTGACATTAAACGTGCTAAAATCGATCGTGATTTTGCTATGGCTCAACTTGAAAAATTTCGTGATACAAAAGAAGAAGTTCAATATGAAATTAAGTTAAGAAAAGCTTTAAACAGGATTGATGTTTATAATCGTAATATCAAATAATTTAGAAATTAATGGACCATTTCACAAGGTTCATTATTTTTTATTTTTTTGTTTCTTAAAATATATTTTTATACTAAAAAAATCATTGCTTTAATGAATAAAGTGCAGATGTAGTTCAATGGTAGAGCTTTAGCCTTCCAAGTTGACTATGTGGGTTCGATTCCCGACATCCGCTCCATATTGAAAGCACAGGTTTGATACAATGAAAACAACGTATCAAGCCTTTTTTTGTTGCTTTTTATAAGCTTTGTACGGCCTTTTTATTAAAAATAGGTATCATTTATCCTGGTTTCCACTAAAAACAAAACAGTGGGCCAGGATTTTTTATTTATTTGACATTCTTACAAAAAAATGAAAGGCCGCTATAAAAAAACGATAGGTTCTTCATATTCGTTAAGTGTTTTTACAATTTTTTTACTTTTAATATAACTGTGAAACGATAGATTTTCATAATAAAAATTATTCGAGAAAAATATGGAAATATTCAGATATTGAAGAAAGCGTTCTACAATAGCGAGAAGATCCTCAGGGTGGTTTCCTTGGCTTCGTTGCTTATCGGATCAGTAACAGGTGTCGTTTGCTTTTACTTTATCCAGTCGATTATACCAGTTGCAAATGTTGCATTGGACATTGAAAGTTATAAAAATTATCAAAAATGTTGTTTTTGGGCAAAATAGTGATATGATAATAGCATAAAGAAGCTTACTTTTAGTATAAATTTTGTTGAAATTTTGACTTATTTTTAGTGCTTCTGTTATAAAAATCACTACCCAATATTTTAGCTTTTTTATTAAACTATTGAATTTTTTTACCTTTTATAAATACGGAGGAAGCCTTATGGCGATAGAAACTAAAGTAATTAACACAAGTATGTATGGAGTATTTACCCAACAAGAAATAATCGACCATTACACTTGTTTTGGATGACAAACTATAAGTGTTACCGATACCAGAGTAACTATGAATAGAAATAATAATTTAAATAACTATATGGAATTAGTTACTCTTGGAAAAGAATATGAAGATCGTTCAAATAGAGCCAATTTAGCCTGAAAAGCAAGCATAATCAATGTTAAATTATTAATTATTTTGTTACTTTTATTCATTGTGGGTGGAGTATTTTACATACTTAACAAAATGAAATGAAGAAAAATTTATAGAAGAGAATCAAATGCTATGCTTGATGCAGTTGAAAAAGCTAGAACATTATATTATGCATATAATTAATGTTTAATTTATAGAACTACACAAGTAATACTGCTTTATGTGCTGCCTATAAGGCCGCACGTTTTTATTTAATGCTATAAGAAACGAGATGTAATTATATTATTTCCAACGATGCTAAGCAATATATATTTTGATAGTGGTATAGTCCTCATTGAACATATATTCATAGTTTGAATCTCTTAAAAATTCTTCAATATATGCTTTTATAGTTTCAGTTCCTTTGCCGATAATGGCTAATATGTAATTATTGCTAAATTCTGAATTATCTTTAGCATTAGCAATTGCAAATTGAATTTCTGCTACTGCTTCATAAATATTTAACCCATGTAAGTCGATGACATTTGATTCTGCTTTATCATAATTGATTATTTTTTTATAGTTTCTCTTATTTTTTCTACTCATAATTTTCTTTCTGGCAATTTGGACAATAATAAGTCACTCTTTGAGGTAATACCGCTTGTTCAATTTTCTCGCCACATACTTCACATGGTTTATTAGTTTTTAAATACACTTTTAAAAAGTTCTGGAATTGGCCTTCAACCGCATTCAGTGATGAATAATCGTTAATTGTAGAACCACCTAATTCAGTAGACTTATCAAGAATTTGCTTAGCATTATTTATTATTTCTTTTCACTTAAATAATGGATTTTATTCGCTTTTGTTCAAGGGCTAATTTTTGATTCAAAAAGAATTTCATTAGCGTGTATATTGCCTATTCCTAAAATGTAAGACTGATCTAATAAAAAAAATTTTATTGTATAATGAACCCCAAATTCCGGACTTTTTGGAAAGGGGTTCATTTTTATGCAATTCAAATTTAAAAAAGAAAAAAGGAAGAAAATCTAAAATAAATTTAGATGCTCAAAAGACAACAAATTTTAAAATCAATATTGAAAACAAAATAGAAGATAAAGATTTACTAATTAAACAATTAAAAGAAGAAAATAAAATACTTAAATTGGAGAATGCGATAGCAAAAAAAATGAGCGCCTTGGTTCAATTGAAAGACTCACTAACAAAGAAAAATTCCAAATAACAATTGAACTAAGGCAAGAATTTAAAAAGCTATTTTCTATTAAATTAATATTAGAAAAAATTAAATTGAAAAAGTCAACTTTTTATGAGATATTAAAATCACAAAATAAACCTGATAAAGATGAAAATTTAAAAAAGGTTATTTTTGGCTTATTTAACTATAATAAAGGACTATACGGTTATAGACGTATTACTTTTGCTTTAAGAAATAAAGGAATAATAATCAATCATAAAAAAGTTCAAAAATTAATGAAATCATTAAATCTTTTTGGCAAAACATTAAGAAGAAAAAATAAGTATTCTTCATTCAAAGGCGATGCTCACAAAAACATTCCAAACTTACTTTTAGAAAAAGAAAATATAATAAAAGATTTTTTCAGACATATAAGAAATTTTTCTAATGAAAAATATTTAGATATATGAGAAACAGATGTTACCGAATTTAAATTAATAAATGAAGAAAAAATATATTTTTCTCCTGTTATAAATTTTAGGACCAGAGAGATTTTAGGTTATTCAATTACTAAATCTCCAAATTTAAGAATGATAGGAGAAATGTTAGAAAATGTAGAAGAAAATGGTCATAGTTTAAACAATGTATTAATACATTCTGATCAAGGATGACAATACACTCATCAAGATTATATTGATTATTTGAAAGAAAAAAATGCAATTCAAAGCATGTCAAGAAAAGGAAATTGTTTGGACAACAGCCCAACAGAATGTTTATTTAGTGTAATCAAAAGAGAATTTTGGTTTGGTGAAGAAAAGAAATTTAATAGTTTTAAAGAATTTAAAACTGCTTTAGAAGAATATATTTCATATTATAATAATGACAGAATTGTTAATAAATTAAAAGGACTTAGCCCTGTTCAATACAGGAATAAGTCCAAACATAATTAAAAAGTCCAATATTTGGGGTTCATACCATTCATACCAAGGGTCTCACTTTTGAAATTAACAATTTATAGAATTAACATTTTTTTGTTTCTTAAAATATATTTTTATACTATAATATCAATACTTTAATGAATAAAGTGCGGATGTAGTTCAATGGTAGAACTTCAGCCTTCCAAGCTGACTATGCGGGTTCGATTCCCGTCATCCGCTCCATTTGATTAAATTCACCGTTTCTTCCTTATTGGAAGATTTTTTATTATAAAAAATAGCCTAAGCTAGGCTACTTATAAATTTTTAATCAACCATTTTGAATCAAGGAACATTTACTTCTTTGATTTCTAAAGGACAAAGCAATTTTGTTTGTTTGTTTTTATTAACATTATCTGTAATTAAAACAATAACATCACTACAAATTTTACTATTTCTTAATTTCTTAAGATCAACAGTTGCTAAGAGTTGTCCTCTTTCAACTTTATCATTTTGTTTAAGATTTGTTTCAAAGCCTAAACCATTTAATTTAACTGTATCAATACCAATATGTACTAAAACTTCAAGACCATTATCATTTGCTATACCATATGCATGTTTAGTTGGATATGACATAATTAATTTTCCATTAATTGGTGCATATACTTCTAATTTGTTTAATTTTTTATCAATTTCAATTGCATAACCACAACCTAGCAAACTAAAGGTTTGATCACTTAAATCTTCAATTGATTTTCTTGTACCTTTGCAAGGTGTATAAACTATATTTTCATCAACAGTTTTTTTAACTTCAACCGGTTTTTCTTCAAATAAGTTGACATCACTTTTAACTTCAATTTTTTTATTTTTAAGTGCTTCTTCTTCTAAGAATGGTGCAGCTTCTTGAATATCTAAATTTAATTTTTCACTAATTTCACCAAATTTAGCTTGCACATGTCTTTTTCCAATAAAAATAACTTCACTTGCCCCAACTTTTTTAAGAGCTTCTTCATTTACTAATGAAGTATCTTTAACATCATATCTAAGACGAACAGCACAGTTATTAAAACCTGTAATATTTTCAATTCCACCCATAGCATTAACTATGTCTCTTGTAAGTTGTGAAATTTCAACAAGCTTCCTAACTTCCTTAATTTTTTGTTTATTGTCTTTACGACCAAAAACTCTTTTAATTCAACTAACAAATCCCATTTTGAATTATCTCCTTGAAAATTAAATCTTATACGTAAGATATACTTAATTAATATTAATATAAAAGTTATCTTTTGAGATTATCTTTAGATTAATAAATTAATATTATATGAAAACATTAACATTAATAACAATAATAATTTGAAAAACATGAATTTAAAAATTAAGTTGTTTCATATAATTAATAAATAAATTGATTAAGTTATATTAATTTAATTAATATGTTTTGTTAGGAGAAAAAATGAAAATAGCTTTTTTTGATGCAAAGGAATATGATAAAAAATATTTTGATGCAGTAAATAATGGACGACATGAAATTAAGTATTTCGACGAAAATTTAAACATTAACACAGTAACTAAAGCTAAAGGGTTTGATGCTATTTGTGCTTTTGTTAATACTTATGGTGACAAATACATTCTTGAACTTTTAGCCAAGATGGGTGTCAAGGTTTGATTACAAAGATCAATGGGATACAACAAAATTGATTTAACAAAAGCTGCTGAATTAGGAATCTCAGTTTTTAGAGTACCTAATTACTCAGCCGAAAGTGTTGCTGAATTTGCAGCTGGAACTTTATTAGCTTTAAATAGAAATATTGTAAAAGCTGAAAAATTAGTATCAAAATATAACTTCTCACTTAATGGTCTAGATGGTAAAGCAATTCATGGTTCAACTGTGGGTGTTATTGGTGGCGGAAAAATTGGACAATGTTTTATAAGAATTATGAAAGGTATGGGAGCTAGAATCTTAGTATTTGATGCTTATAACCAAAAACACTTTCCTAATTTAGCAACTGAATTAGGTTTTGAATATGCTTCATTTACTAAAGTATTAAAAGAAAGTGACTTTATTTCACTTCATGCGCCTTTATTACCTTCAACAACTCACTTAATTGACAAAGATGCAGTTAAAATAATGAAAAAAGGTGTGATCTTAGTTAATACAGCTAGAGGTGAATTAATTGATATTCCCGGTGTTCTTTATGGACTTAAAAAAGGAATTATTAGAGGACTTGCCAGTGATGTTTTAGAAAGAGAAGAAGGAAGATTCTACCAAGATATTTCAAGTGAAGCAAATGAATACAAAAAGAATGATCCTGAATGAGAAGAATTAATTAGCAATGAAAATGTTATTATTACTTCTCACCAAGCATTCTTAACAGATGTTGCTTTAACTCAAATTGCTAAAATCACTTTAGAAAATGCTGATATGGCACAAAATAAGGACTTTTCTAAAGCATTAGTAATAATGGAAAATGGAAAAGTTAAAAACGGATAGAAAATAATCCTAATAAATAAAATAAAATTTGTATAAGCCTGTTTTATAGGCTTTTTTGTAAAACTTATTTATTGAAAAAGGAGAATATATGTTTAATTATTTTAATTTAAAAAATCGTCAGGCTGCAGTGCAGCCTACTAAAGCGATTCACTGATTTAGTCACTTATTTAGTGAACTAATTGGAACGATTTGAATTTCACTTGGTTTAGCAGGTTTATCAATTATTATTAAAGATAAAGTTGTTGAACATTACTTATTACACAATGTTATTGTTGGCTTTTTTGCTGGCTTTGTAGTTGTCGGATCATGCTTAATTTTCTTTGGCAGATGATCAGTTGATTTAAACCCAGTAGTTACACTTTATCGTTGATTTAATGGTACTAATAAAACTGGTTATGTTATGGCTAAATTAGCTATGCAAATAGTTGGCGGAATTTTAGCTGGTTTAATTATTTATGGTATTGGTCATAATGCTTCTGGAATAAAATTTGAAGCTAATTATCTAGCTAATGCAACTGATACAGCACATAAAGCTAACCACTACATTTCAGCACACAATGTAAGCGCAAAATCATTTATGCCTAACTTTGATAGTGCTTTTGCAAAAGAAGCGGTAATTACTAAAAAAACAATTTGAAGTGGTGGTGTTTGAATCTTCTTTGGTGAAATGGTTTTAGGTGCAATTCTTTTATTCCCTATCTTTAGTCCAAGATTAGACAACAAATATCGTGACACATTTATTTGCTTCATTATTAGTTTTGATGTTTGAATGGGTATTCTGCTAGGTTCAGCTGCCATTAACCCTGTTCGTGGTTTAGCTCAACAAGTACCTACCTTATTCTTTGATATGAAACACTTAAGTGCTATGGCTCAATATGACATTCACATGGGTACTTGAGCAATGGTTTTAGGTAACTTAATGTCACCAGTCTTCCATGCATTCATGCAAGGATTCACTGAAAAATATGGTAATCCAGCTTTACTTTGAATGTTAAATTACAAAAATAATAAAGCTAGTCATTATGTAAAAGAAACACAAACAAAAGAAGAAAGCGTTAAAAAAAACAATTAATTTCAAGATAAACTTAACAACCTAGTTATGTTTATTTTTTATTATTTATGCTTCTATATAATAAAAAACAAACAACAATATACTTTCTTTATTTATATACAATAGAGCCTGCATCTGTGTGAAAAATTTTTCAAAAAGTTTTTGCATTCGAAAAAAAGCGTATATAATTATTGCGTTATGTTAAAAAATTGAAACTTTATTCGTATTGAAAAGAATATCAACATTATCAGAACAATTATGATGATGTCTAATTTCTAATTCAATACCTCTTAAAGATCAAAATGTAATATAAATTTTGTAGTTTTTAAGCAGGTATGCAACAGAAACCTGCTTTTATAATAGACGCTATAGAGGAGCTCTTAGTAATAGTATATTAGTTTAGCAGGCATGTGTTGAGACTATATAAAAAGGATATAAGAGCCGAACTGGTGATTGTGGCGGCAATTACTGGTGGTAACAATGCATAACAGTTTGTTACTCTTTGGACCTCAAACCATTGGTGCTATAGCTTTGCAAAAATAAATAATTTCTTTAATTATTATTTTGTAGAGTATTTAGTGCTTTACAAAATTTTTATTTAATCATAACATTAATTATTATTAAAAAAGATATTAATATATCGAAAGGAAATTATGAATAAAAAAAGAAGATTATTATGATCTTTTGCCCCATTAGCTACATTAGCTGCTTTTGCGCCTGTTGCAATCAGTGCTAGTTGTGGAAGAAAAGGCCGTGTAATACCTAATCTTGACTTAATTAAAGGGCAAACTAACTGAAGTGTTGTAAACTCTAAATATACTGAAGATTCATACACTACAGACCTTTCAACAACTTATGGTGGTTTCTTAAATAGATTAGAAGAAGAAACAGGAGCTTATTTACTTACAACAAGAAGTTTCGGAAATTGCAAAATTGTTCAAGCTGGTCCAAAAGGGCATGAGCGTAAATTTGTAAAAAGTCCTTCATATTGAAGATACTCATTAGAATATGCAGATGCTGTTGTTGTTACAAAAGCAGATGGTACAGAAGCTGTATTTGACTCAGATGAAGCTGAATTAAAACAAACTCCAGAACATCCTGAAGATAAAGCAAAAAATATACCTGCACACTTTGATTTAGCTAACTATCATGCTAGATCAGCAAATGCTAGAAGTGTTAATAGTGCTGAATTTGAAAAAGCTCTTAAAGGAGCTAAAAAAATCCAATTGCGTGTAAGAAAAGGTGCTAAATGAGTTGATTCATTAGGCAATGAAACAAAATATGATGTTGTTGGTGATGACTTCTACATCTCATGACTTAGAACTTACACACTTTCAACTGGTGTACGTAATAGTTACTTAAAAGGTACAAAATATGAAGACAAAGTAGCAGAACTTGATAAACTTGCAAGCGGAATGCTTTCAAAAGCAACCAAAAACTTTACAAATGCTTTAAGATATCCTAACCAATATCTATATGGTTTATATGGTGTAGATAGCAACAAATTCAAAAACAGAAGTGAATTCTTAACTGAAAAAGATGGAAGGGAATTTATTAACTTTACACAACTTGCTGGTAAAGAAGATATTACAAGTTTTCAAGACTTACTTTACAATGTTGCAACATCAAAAGATTTTGTTGCAGCACCCTCACAATACATTAAAGAAACAATGAATTCACCTAAAATTTCTGTATGAAATGGTAAAAATGGAAGTAAAAGTAAATCAAACTTATTAAGTGCTTTATCTGAGCTTCCAGAAGATAACTTATTAAGAGTTGCTGGTGTATATACTTATGGTGCTAGTGAAAAAGACATGCTTTATGCAGGTAGATACTTCTCAGCTGGATACTACCCAGAAGCTTCAGAATATAGATTCTACAAAAATAGATTCTATTTTGATAAAGATTTTGTTGAATCAGAAAAAAACTTTGGTAGAGTTGTTACAAAATACTACTCAACAACTGAAGATGTCTTTGTTGCTCAAATATTTGATCTATATCAAAAAGGTAAATTACATACATTATCATTTGCTCAATTAAACCAACAACAACAACTTGATGTTACCAAAAATCCTCAAAAATATGGACTTGAATATCGTCAATCATTAAATAAATTAAATTCTCCATTTAAATTATTATTTAACACAATCCCTTATGGTAAAAACTTCAATGCTGACACTAAAAACCGATTTCTTAATGTTCATGCAGCAAAATTGTTCTATGGTTTAACAGATAATCAAGTACATAATGGAACAACCCAAGAAGAAACTAATGAAGACCTTTATGTAAACATGCTTTCAGGTAGATCAGTTGCATTTAGATCATTATTAACAGCTGCTGTTAACTGAAATGCTGTTGCTAACACATTATCAAACAATAAAACTAAACCTTGACTTGCTGGTGTTGCGCCTCATGGTAAAATTGGCGGTTCAGATCAAGATACAACAGCTGCAGCGAAAAGACCTGTTGACGATTATGAAAACTTAAACACATTCTACACAGTTGATTCTAATGGTGAAATTGGTATAACAGTTACACCTACAGAAAATGCTACAATTGCAAACACTGCAAGTACAACTGAAGAAAAATACAAATCAGCTAAATTTAATGAAGTTAAAGCTGCTATTAAAAAAATTCTAGATGATTATGAAGCTACATTGCCAGCTGGTGAAAAATTAGAAGTTGAATGAACTGTTCCAAATAGATATATAAACTGAAATCCAAGTAACATGGAAAAAGTATTCAAAAGTTTGGAATCATTAATTAAAAGTATTGATCCAAGATTGAAACCTTCATATAAAAAATTTGAAAGTTCAGAAAGTGACAAAGCAAAATGAGGTGATTCATTATTTGGTGGATCAATTGCCAAAATTATTAGTTGAGGTTATGATATCGATAACATCGGTTCAGGTATTGATGGTATTACAAACAGTGGATTTATCGGAGCATTATTCTCAATTGGTACAAGTGCAAAATTACAAAACAAATTACAAAAAGCTTTTCCAACATTAGTTCAAGCTTCAAAAGAACTTGTTGAATTTGCTTTAAAATCAGAAAATAATTTTAAATTTGGTTTTAACTTACATGACTTCAATAAAATACCAACAAAATTTTCAAATGATTTAGCTGAAAACCTTCACCACTTAAAATGAGATTCTAAAGAAAACAAATTTGTTTTTGACGAAAAAGAAAACTTCACAATGTCATCTGTTATTTCTTCACAATTCTTCGTTTCTTATGTAAAAAAACATACAAATGCTCAAGTATTAACCTTAACAAAAGAAATTATGAACTATATGGGAGTTGTTGTTAACCCTGCTAAAACAATTGCAAATGAAAACTTCGGTCCTACTTTAATTAATAGTGAATATTATGAAGTACCTTTTGCAGCAGATAACAATTTATGATTAGCAGATATAATTGTTAAAAATGTAAAAAAATAGTGTAAATATATATTTACAAAAAAACTATGGTTAAGTATATATTCAAGAGAATAGCATTTGCTATTCTCACATTATTTATTATTATTATATTTTCATATTCGATTATTATCGCTTTTCAACAAAACCCTTATCGTATCGCAGCTTTAGAAGAAGGACTTTCAAAATTGGAAGTTCAACAAAAACTTCAACAAGCAGAAGAATTCGATCAAACGCCTGGTATTGTTAAATTATTTAACTATTTAATAAACATTTTCCAATTTAATTATGGCGAAGTTTATGTTTATAAATCAGAATATGAAACAATTCCAGAAATGTTTTTTGAACCCTTACAATGAACTATTTTAATTTCATTACCAGCTTTCGTTATTAGTGCCATTTTAGGTGTGGCAATTGGCGTTTTAGCTGGATATAAAAGAGGAACAATTTGAGATACGTTGATAAACATTTTCATTTTTATCTTCGTGGCTGTTCCTTCATTTGTTTTAGCTCCATTCTTCCTTAATATCTTTAACAAATTAGGATTTGGCCGAACATTTGTTTCACCATATGAAACAGATAGTTCATGAGCTAAAACAATTAAATCAATTATTCCTCCAATTACATTATTGACTTTAGGTTCTCTTTCAGTTTATACTCTTTACTCAAGAAATCAAGTTATCACAGTTTTAACGTCAAACTATATTTTGATAGCTAAAACTAAAGGTTTAAGTGGTAGCAAAATATTCTTTAAATATGTATTTAGAAATATTTCAATTCCACTTGCAGCAATAATAATTCCTTCATATATTGGTCTATTAAGTGGAAGTATTATTATTGAAAGATTCTGATTGGTAAAAGGTACCTCAGATCTAATCGTTAACGCCTTCCCTAAAGGTGAGACAAATATTGTTATGTTTAACATTTTATTCTTTACCACATTATCCTTATTTACAGAGGTTTTAGTCGATGTAACATTTACATTGATTGACCCTAGAATTAAGTATTCAAAAAGTGGTGGAATTAACTGATTTGAAGTTATTAGAGCTTCAAGATTCCGTAGAAAAGCAGAAAAAGAACTATTCAAGCAACAAGATCAAGAATTATTAATGAAAGGAGCGAACTAAAACATGGATAGTAGGACTTTTAATGATAAGTACAATATTTCAGATGAATTAATTCAAAAGATTAAATTAGCTGAAAAAGAAAAAAAGTCAACTAATATTGTTGGTAAACCAAAAATATTGTTTTATGAAATTTGCAAAAGATTTTTCAAAAATCCAGTTGTGCTTATTTCATTTACAGCGTTTATTGCTCTTTTATTAACTGGTATTATAGTTACTCTTACTTCTCCTTATTCTCCAACTGGTTCAATATTAAAAGGTGGTGATGAATTAACTCAAAATTTACCTCCAGTCTTTAGTCCAGTAATAACTAATGCCAGCAATTACGAAACTTTTCAAAGAATGAAAGAATGAGAAGGCTATCATTATATTGAGCCTTACTTAAAAGATGTCATAGTTAATAGTAAAGAAAAATTACTTATATATAATGCCTATGATTTTTGAAAAGCTTATTATATTTGATCACGTGCTTTAAAAATTAATCCTTTAAAACCAGAAGATATAGAAAACATCAAAGCTTCAATGCCACATTTAAATACTCTTTTTGGTACAACAGTTAAAGAAGCAGATATTTGAACAGGCGCTTGATTTGGTGCAATTAGCTCAATTAAAGTTGCTTTAATTGTTGCAACTATTGAGGTTGTAATTGGTGTTGCTGTTGGTGCTATTCTTGGTTTCCATGCTGGAAAATGACTTGACACTGTAATGATGAGGGTCATTGAAATTTTCCAAGCACCACCTTCAGTTATTTGATTATTGCTATTCATTTCTGCTGCTGGAACTAGTGAATGAGTCTTGATTGCTGGATTGCTTTTTGTTGGTTGAACTTGACCTATTGGTGCAACTAGAATGTTCATTATTACAGTTAAAGATGAAGAATATATTATTGCTGCCAAAAGTGTTGGTGCAAGCACAAGTCGTCAAATATTCTTCCATGCTTTACCAGCAATTTTAGGTAAAATTGCTATGAACTATGTTCGTAGAATTCCATCAATTATTCTTTCTATAGCTTCTTTAGCATTCTTAGGATTCTATAACAAAGATACAGATATTAACTTAGGTAAATTTATTTACGATAACATTAATGATGCAAAAACTAATCCATGATTAATGTGACTTCCAACTCTTATATTATTGACACTTTCAGTTAGCTTACAATTTATTGCTATTGGTCTTCACGATGCTTTAGACCCAAGAGTTATTAAAGTAAAAGGATAAAATTATGACTTCAGTAAAAGAAAAAAAGAATATTAAAAAGAGAAATGAAGACATTAAAGCTGAAAAATTAGCTAATGATTTATTACTCAAAGTTCGTAATTTAAGAGTCGACTTTAAAGTTGGAAGAAACAAATATTTAAGAATAGTTAGAGGCATTGATTTAGATATTTTCAAAGGTCAAATTGTTGGCCTTGTTGGTGAAAGTGGTAGTGGAAAGAGTGTTACTTCAAAATCATTAATTAATGTTAATGAAGGTGCCACTACAACAGCCGATTTTATGCAAATCGAAGACATTAATTTAAACAAATTCAAAAAAGAAAGACAATGACAATCAATTAGAGGAACATATATTGGATATATCCCCCAAGATCCTCTCACAAGCTTAAACCCTACTAGAAAAATAGGTCTTCAATTATTGGATGCACTTAATCTAAATAAAGAATGAAAAGGCAAGAGCTACAAAGACAAAAGAGCCTATTTGGTAGGCCTTTTGGACAAATTTGGTATTAGAGATTCTGAACAAGTTTTTGACCGTTACCCACATACTTTAAGTGGTGGTATGAAACAACGTGTTGTTATTACAATGGTTGTTGCATTAAAACCAAGCTTAATTATTGCTGACGAACCTACAACTGCTTTAGACCCAACTGTTCAAGCCTCTGTTCTTGCTTTATTCGAAAAAATTCGTCAAGAAATGAATATTTCAATTATTCTTATTTCTCACAACATTTCTGTTGTTGCTAAATTTTGTGATTATATTTATGTTATGTATGCAGGAAGAATAGTAGAAAAAGGAACACGTAAAGAAATCTTTACTGACCCTTGTCATCCTTATACTTGAGCTTTAATTTCTGCTATTCCAGAAAACTCACAATCAAGACTTTACTCAATCAAAGGAACACCACCTGATATGGCTAACTTGCCTCTTGGCGATCCTTTTGCTCCACGTAATGATTATGCTTTAGAAATTGATTTCTATAAAGAACCTCCTTTAATTGAAGTCAGTGAAACTCATGCAGCTGCAACTTGATTATTACATCCAGATAGTCCAAAAGTAGAAAGACCAGAAGAACTTGAATATAGACTAAAAAGCTTTAGAAAGGTATTTAAAGATGAATAAAAATATTGTTTTAGATATTAAAGGTCTTAAAAAATACTTTACTAATAATGGAATTACAAATAAAGCTGCAGATGATATTACATTCAATGTTCATGAAGGTGAAATAGTTGGACTTATTGGTGAAAGTGGTAGTGGTAAAACTACTGTTGGTCGTTCACTTTTAAGACTTTATGATGATTTTAATGGTTTTGTAACACTAGATGGAAAAATTGTTAGTGGTGCAAGGATTAGTAGAAAACAAAGAAAACATTTGAGAAGAAATATCCAAATGATTTTCCAAGACCCTCATGCTGCTTTAAATGGTCAAAAGAACATTTATTCAACTCTTAAAGAACCACTTGAAGTTAATGGAATTATTAAAGAAAGAATTCAAGATATCTTTAAAGACTGATTTGATATTAAAGAAAACTTCTTTTATGTATTTAAACAAAGAGCTTTAGAATTAAAGCTAAAAAATCTAGAATCAATTAATCTTTTAGCTGAAAAATTCTTTCCATATTGAATTGAAAAATTCAAGAAGATGAATTATGATCAAAAGTTAGATATTGAAGACAATTTCAACAACTTTTATAACTATCTTGAAGAAAAACAAAATATGGAAAGTGAAATTGTTGACAACATGTATTCAAATACAGATAATCTTATTAATTTTTATTTTGAAAAACAAAAAGATTACCGTAGCGGAAATTTGGACTTTGATGAAACTAAGTTAATTAAAGCTAAATCAAACTATGAAGAAATACTTGAATTAACAAAAATATCAAAACAACTATATGAAGCAAAATTACAACTTGCTGAAAAAAGTAAAGAGTTAAAAGAATTAATTAAAAACTATAAAGAAAGAGTTAATGATAATAAAAACTCATTTAAAAACTTTATAGCAGAATACAAAAGTGAAGCTAGAATTTTTAAAGTTACACGTCTAACTTCTTATGATCTAGATTTTTACCTATTTAACTATAAAAATGAGTTAATCAACAAAAAAATAGTTAAAGAAATTAGTTTGATTAAGCCTAACTTAAAATTTATCGAATACCACAAAGTTTGCGACTTAATTGAAGATTTAAAAATTTATTCTAAAGAATTTTTTGAAAAATATTTAACACCTATTAAATATCAAAGAAACGTTATTAAAATTGTTGAAAAAACTATTAAAGACAACTTCAAATTTATATATGAAAAATATTCATTGCTTAACCAACAAGAAAAAGATTCTCATGAAAAAGAAGTTGCTACATTAAAAGAAGAAATCAAAAAAATAAAAAGCTTATTAAAAGAAAAACAAGAACCTTCAAAAACTGCTGAAGACTTAGAAAAAGCAAAACAAAATCTAGATAAAGTTAATAAATTCCATGAAGAAGAAATTGCTAAATATGTTGAAAAAAACAAATTAGAAATTGATGAAATTCAAAGGAATATTAAAGAACAAAGTGATAAATATAATTCACTCAAATCACAACAATCAATCACAGACTCAGAATTCAATAGAGTTAAAGATATATTCTTTAATGAGTTAAATAGAGTTATTGATGAAACCAAAAAAGTTAATACTCTTGAAAATAAAGCAAAAGTAAAAGAATTGACAAAAATCAAAAGTATTTTTAAAACAAAAATTTATGATAAATTGAACACACTTAAATCATTTAACATTGAAGTCAAATATCTTAACAAAGACATTAATGATATTAAGAAACTTCTTGGTATTAAAAACTCAAAAATGATTAAAGTGTTCGGAAATAAAGTTGGTAAATTCATTCAAAAACATTTTGCTAGATTTTATTACCATCATTCAATTTCAAATCTTTTAACTAAAACAACAATTTATAAAGCTTTAGAAGATGTTGGATTACTTAAACAATTTGCTTATCGTTATCCACACGAATTCTCAGGCGGACAACGTCAAAGAATAGTTATTGCTCGTGCTCTAATTATTCAACCTAAAGTAATAGTAGCTGATGAACCTATTGCCTCATTAGACATCTCTATTCAAGCTCAAGTTGTTAACTTATTAAAAGACTTATGCGATAAGAAAAACATTGGTATGATCTTTATTGCTCATGACTTGTCAATGGTTGAATACATTGCTGATAGAGTACAAATTATGCACCTTGGTAAGATTGTCGAAAGTGGCGACACTGAGGCTGTTTATAATAAGCCTATGCACCCTTATACAATTAATCTTTTCAAAGCTGTACCTAAAATTTCAAATGCAAATGAAAAATTTGAAAATGTAAGTTTTGAATTAGATTACCTTAAAGGTCAAGTTTATCCAAATGTTCCTCAACTTTATAGAGTTGAAAATAATCATTATCTTTATGGAACAGAATCACAAGTTGAAGAATGAGTTAAACCATTTAATATCAACAGTTTTTCATTAGCAACAAACAAATCAATTGATGAAGAATACAATTCAAAAATAGATCATAAACCATACGATGGACATAGCGAGATCCCAAATAATATCGAATATACAGATGAAATTAAAATAGACGAACCTACATGTGAAATAAAAATATCTTCAAATTTTGACTTCTCTAAACCTATAGTTGAAGTTGTAGAAACTAAAGTTTCAACAACCAAAACAACTAAAAAAAGAAGTACTTCTAGAAAAGCTTCAAGTGCTAAAAAAACAACAAAGAAATAATTTTTGTTAAAATTTCATTACTATGAATTCAACAAGAGAATATTTTAGAGAAGCTTTTACTTGAAAAAAACTTCTGCACTTATTTATTATTTTATTAATATCTTTAATAGCAGGTGTTTCACTTTATTTGTATCGTACATATAAAACTGAAATTCCTTATAAAACTAATGTTAGTGACACACTATTACTAATAGGCGCAATATTGTTAGCTTATTCAATTGTAATCATTTTAGTCACCTTAGGTTTTGGTACCGCCTTATTCAAAAACTTAAGAAATAATTCTTTAACAAGAACTAAAAATGAATTAGAAGCTGAAAAAAGAAAACCAGCTTCAGAAGAACAAAGAGCCAAAATAAAAGTTCTTGAAAAAGAAATTGAAAGAAAAACTAGAAAAATTGAAGCGAGCGAAAACAAAAAAATTAATCGTTTTATATATTATTTGATGCTAATAATAGGATCGATACTCCTTATTTCCAGCGCCATAGTGGGTTATATGTAAAAATCTAAAATGCACTAAGTGTGCATTTTTTGTTTATAAATAAAAAACAAAAATATAATATAATTAAAATAACTTTTTGAAAGGAGATAAAATGTCAAACTTATATCAAACTAAAAGAGACGATAATATTTTGTTAAAAGCTTACTATGATGCAAACAAAAATAAAGAAATTAAAATTGACCAATTAAACTATAAAACAGGCAAAATAACCGAATTTTTAAATAAAAAAGAGGCTTATATTTTCTTAGATAAAAACTTCAAAAATTATGATCAACTTGTTGATTTAATTGATACAAAAATAGTTACTAAAAATAGAAATTATCAAATAGATTTAGAAAGCTTTGTTAATAAAGATTTTCCTATTGAATCAGTACTAAAAGCATTCTATTCAAGAATCATTTTTTACACCTACAACTACTTCAATAAGAAAAAAGAAGCACCTAAAAAGAATCAATATACATTTTTATTAAAAGATAACTCACACAAAGAATTAGCTAACAAATTTGAACTAATTGCAAACAATAGAAACTTAGTAAGAAACTTACAAGTTATGCCAGAAAACTTCTGTAATTCAGAACAATTAGCTCAATACATAGTTAATGATTTTAAAAATGAAAAAGATTTAAAAGTAACTGTTTTAAACAAAAAAGAAATTGAAAAATTAAACATGGGTTTAATTATTTCAGTAAACAAAGGTTCAACACATGAACCACGTGTAGTTATTGTTGAATATAATGGAGACCCTAATAGTAAAGAAAAAATAGTTTATGTAGGTAAAGGAATTACATTTGATACTGGTGGTATGAATACTAAAGGTTATCACATGGAAGGCATGAAATATGACATGTCAGGTAGTGTTATAGTTGCTTACACAGTTAAAACATTAGCTCAATTAAAAGCTAAGAAAAATGTAGCTGCTATTATGTGTATTACTGATAATAGACAAGATGGTGATGCTTCACTTCCAGAAAACGTTTATGAATCAATGAGCGGAATCACTGTTGAAGTAACTGATACTGATGCTGAAGGTCGTTTAGTATTAGCTGATGGTCTTTACTATGGAGCTAAAAAACTTAATGCTACAACTCTTGTTGATGTTGCTACTTTAACTGGAGCTGTTTTAAGAGCTCTTGGAACAGTTTATTCGGGAATTTATTCAACAGATGAAAAGAACTGAAAAATATTTAATCAAGCTGCAACAAATGCCAAGGAAAAAGTATGAAGAATGCCTCTTCATGAAGATTTCCATGAGCCAAATACTGCTTCAATAGTTGCTGATTTAAATAACTATAATAATGATGAAAAATCAGACTGTAATACAGCTGCAATGTTCTTAAAACAATTTACAAATGATGTTCCTTACATTCACTGTGATGTTGCAGGTACTGCAGACCTTAGTGGTAAACCACAAGGCGTTTTAGTAGATACTTTAGTTGAATTTGCTTTATTAAAATAATATATAGAAGACTTTTTCTTAATTGAATTAGTCTTTTTTATATGTTTTTTCAAAATTTCTATTGAAAAAAAGTGGTCAAATGACCATAAATTTTCAATAGAAATAATCAAAAATAGTAAAAAGTGAAAATTCTACTGAAAAAAAATGGTCAAATGACCAGAAATTTTCAATAGAAAATAATAATTTAGTATAATTTACATGAGGTTTTTATGGAAATAAAAAGAGATTTTTACTTGAATAAATTAATAAATAAAAAACATAATAAATTAATAAAAATAGTTACTGGTCCAAGAAGAGTAGGTAAAAGTTATTTACTCTTTAATTTGTTTTATCATCATCTTTTACTAGAAGGTGTTAAAAATGATCAAATAATAAAAATAGCTTTAGATTCTATAGAAAATGAGCATTTAAGAAATGGCAAAGTTTTATTTGATTATGTCTTAAATTTTATTAAAGATGAAAACAAATATTATTATGTTCTATTAGATGAAATACAATATGTTTCAAAATTTATTGATGTTTTGAATGGCTTAATGAAACGTGAAAACATTGATATTTATGTGACTGGTAGTAATTCAAAATTATTATCAAGTGACATTGCAACAGAATTCGCTTCTAGAGGTGATGAAGTTAGAATATATGCTTTAAATTTTCAAGAATTCAAAAGCATTAATTTAAATAAAGATAATGAACAACTTTGAAAAGAATATTGTGAATTTGGTGGTATGCCAAATACTGTTTTAAATGAAAACAGTGAAGAAAAAGAAAACTTTTTGCAAAACCTTTTTCAAAATACATACATGAATGATGTGATTTTAAGATATAAATTAAGAAACAAAGAATTTCTCAATGAAATAATAACTTTTCTTGCAAATAATATTGGATCATATATTAACACCAATAAAATATTTAATTATTTTAAATCATTAAAAAAGACAAATATAAGTTACAACACAATTAGTAAATATTTAGTCTATTTCAATGAAGCTTTTATTATTGAAAAAGCAAATAAACAAAGTGTTAAAAGTTATGAAGTGTTTAAAAGTTTAAACAAATACTATTTTGTTGATCTTGGTATTAGAAACTTTTTAACTAATATCTCTTCAAAAGATGAAGGACATATAATGGAAAATATTATCTTTCTAGAATTAAAAAGAAGAGGTTTCAAAGTATCTGTTGGTTCTCTAGAAAAAAGAGAATTAAACAGTAAAAATGAGTATATTAGAAAAGAATTTGAAATAGATTTTATTGCTAAAAAGAAAAATCAAACTTACTATATTCAAAGATGTCTTAATTTAAATGAAAGTAATGAAGAAAGAGAGAAAATACCTCTTAAAATGCTTGAAGATGCTCATCGACGAATCATCATAACTAAGGATGCTTTTACCTATAAAACTGATAATTACGGCATAGAAACAATTAACTTATTCGACTTTTTATTAAATGATAACATTTTTTAAAAAAGCTTATAAAACAATTTAATTAGTCAAAATTAAAATTGCAGATTTTACTGTCTGCAATTTTAAATTCTTGTTTGATAATTATTTTTATCATAGTTAATTTTTGCAGTCATTTTTTCTCCTTTTTTATATAAAAAATTATAAATTAAAATTTTGATTTTTGGAAAAAATGGTATTTTGAAAATAATAAAAAACAAAAAGCACTATAAAATAGTACTTTAAGTAAAATGTATGTGATTAAAAATTATTGTATTTTTTAAACACACTGTAAACTGCATCAACTGCTAAATTGAATGCTGTTTTGTGACTAAAATTATCAATATCAGGATTCTTTTCTGATTTCTTTAAAAACAATTCTTTTGTTTCTTTTCAAATTCTGTCATATTTTTTAATTTCAGCAACTTTATCACCAGTATATTCATAGTCATAAACCTTTGTTCAATTGTAAACTATTTTATTAGCTTTATCTTTAGATCTAACTAGAACAAATCTTTCATTATTTTTATTTTCGCTGTATAAAGCAATATCAATTGAAAAATTTTTGAAATCAGTAAATTTTAAATTGAAACTTTTTGAATTTTCAACAATTTTAGTACTAAAACCTAATGGACAAATATGTTTTTGTAGCTTTTCAAGTATATATTTGTTGAAATCCGCACTTTCTAAATTAATAGTTTTTTTAGGTTTTAAAATAATGTTGAAGTCATATGCAAGTGTGTCATCAACAATAGCTAACATTTTTTGACCAAATGGAAAGGTTTTTTCAACATAAAAACTAATTTCGCTTTTTTGATTGCACATTTCTTCACAAATATCGTCTAATTTATGTGCAACTCTCGTAATTAAATCTTTATTAATATTGTTTGGGAATTTTACTTTAATTAAATTTAAATCTGAAAAATAAACTGCTTTAGCCATAATCTTCCTCTTTTCTAATTTCTTTCATAATATCAATTATAAATGAAGAAAAAGTAAATTATTTAATTTTGCAAAAATATCTATAAAATAACCATATTAGTAAAAATTTAGTTCTTAAATAAACCTTAAAAAATGTTAATTTCAAAAGTGAGACCTCTAGGTTTTAGAGATCTCACTTTTTAGATTGTCCTTTATAATATAAATTACTTAAAAAATAAAGAAAGGACACTTAAATTATAATTTACGGTGCAAATAAAAATGGTTATTGAAAGGCATTAATAAAATAATAGTTTAAAATATATTATTAATAAAGGAAGAAAATGAGTTATTTACAAAAGTTATTAGATAAATATTGCCCAGATGGATATGAGTGAGTTAAATTAGAGGATGCAGTTGAAATTTTTGATAACAAAAGAATTCCTATAGCTCAAAATAAAAGAATAAAAGGAAAATTTCCTTATTATGGTGCAAATGGTATTCAAGATTATGTAAATGATTTCATTTTTGACGGTGAATATATTTTAATAGGCGAAGATGGTAGTGTTATTGATGGGTTGAATCACCCAATTTTAAATTATGCAACAGGCAAATTTTGAGTTAATAATCATTCGCATGTTATAAAAGCAAAAGAAGAATTTTTAAATAGATTTATCTACCATTTTTTATCTATTTTAGACATTTCTGATATTGTAAGAGGCACACCACCAAAAATGACAAAAGGTAATTTATTAACTATTTTAATACCTAAAATTCCCTTAAAAATTCAAGAAAAAATAGTTGAAATCTTGGAGAGATTTCGTATACTAGAAGCGGAGCTGAAAGCGGAGTTGGAAGTGAGGGGGAAGCAATTTGATTTTTGAATAAACAAATTGTTAAATTTTACCAACTTTGATAAAAATAATTCAAAAGAATTACAATCTATAGGTTGTTTTATTAGTGGTTTAAGATCAAAAAATAAAGATAGTTTTGTCAATGGAAACCAAAGATACGTTTCTTATTTGGATGTTTTCAATAACAAAGAAATTAATTATTTACCTAACAATTTTGTAAAAATATTTGATGATGAAAATCAAAACGATCTTAATTATGGCGATGTTATTTTTTGCGGTTCATCAGAAAATTTTGATGAAACAGGTTATGCATCTGTTTACACTATAAAAAATGATGAAAAAGTTTATTTAAATAGTTTTTCATTTATTTTTAGATTTAAAGATAACAATTTATTTTTGCCTAAATTTTCTAAATATTTTTTTAATTGTAAAGACTTTAGAGACTTACTTCTCAAATGTATAAATGGTGTAACTAGATTTAATTTATCAAAAGAAAAGATGTCAAAAATAAAAATACCTATTCCTCCTATTGAAACCCAAAACAAAATAGTTTCAATACTTGATAAACTATCAGAATATTCTCAAGAAATTAATTCAGGTTTACCTGCTGAAATAGAATTAAGAAGTAAACAATTTAAGTATTACAGAGATCAATTATTAGATTTTAAATAAAATAAAATTTATTTGAAAATAAATGTTTTTTATATAATTATTTAGAATAAAAGGACTTATTATGAGAGAATTGATAACTTTAAAAAAGAGTAGAAAAACGCTAACCAATAGGTTAGCTTTTGCTTTCTTTCTTTCTCTCTCTCTAATCTATTAAAAAATATTATTTTTAATTAGTATCCAGCACACACTGGATGTTTTTTAGTTACCTTTTATTCAATTAATTTGTATATAAAAGGAGAAAAATGAAAAAAATTAAATTATTAACAATTTTACCTACAGCTGCAATAGCCTTGCCTTTTTCTGTAATGAGCTGTGATGGCGGTGGACAAGAAAATAATTCAAGTACAACTACAAAACTTGATCTTTCGGCTGAATTAAATAGCGTTGTAGTTGAATATGACGGTAATAAATCAATTTTAAAACCATCTGAAGTTAAAGTTGAAAAAATTAGAATTACAGGATTTGGATCAGAATATGAAGTTGAGCCAAATTCAATTAATTTAGTTCCTCATGATGATAAAGGTACTTTAGAAGTAACATTCAAATTAAGACAAAAAAATCTAGTTTCTAATGTACAAAGTAAAACAGTAGAAATTAAAAACTTTTTAAATTCTTCTGGCAACGTTGATAAATATAAAGGATATGAACATCTTGTTATTGAAAATGATATTGTTAAAGGAGCAAAAAGTATTCCAGCTGGTGGAATTTTAGAAATTCCAGATGGTGTTACAAGTATTGCTGCAGCTGCTTTCAGTTTCAAAGCCGGAAACTTAAACACAGTTGTAGGATCTAGTGTTACTAAAAATCAAATTACCAGAGTTATTATTCCTGATAGTGTAACAACAATTGGAGCAGCAGCTTTTGCAGAAAATGTAAATTTAGAATCGGTTGTTATGTCTAAAAAAGTTAATAAAATCTCTGAAGCATTATTTGAAGGTTGCGTCAGATTGAAAACTATTAATTTGCCAAATGAAATAGACACAATTGGGAACTATGCTTTCTTAAGATGTAAAGCTTTAACCAAAATTGACTTACCTAAAAATTTAACCTCACTTGGATCAAGCATTGATTTAACAGCTGCTAAAACTTTCCAAGGAACAAGTTTAACAGAAATTACAATTCCAGCTAATGTTAAAATGATTCCTACTTCAACATTTGAAGGTTGTGAAAGCTTATCTAAAGTAACTTTAAATTCAAAATTAGAAAAAGTTTCTTGAGCTGCATTTAAAGGATGTACATCATTAACAGAAATTAATCTTCCTGAAAATGTAAAATTCATTGGTAAACATGCATTTTTAAATTGTTCAAATCTTGCAAAAGTTGAAATGAAAAACAAAGTTACTGAAATTAAAGAAGGCGCTTTTGAAAATGTTACAGCTTTAAAAAATATTACATTAAGTACAGCTTTAACTACTTTATCAAAAGAAGCATTCAAAAACAGCGGTATTACTACAATTGCCTTACCAGCTAACTTAAAAGCTATTGAAGAAGGAACTTTTGAAAATTGTACTGCTTTAACAAAAGTTACAATGGGCAACAACATAACTGCAATTAATTCTAAGGCTTTCTACAACTGTGGTGTTTTAAATTCAATTAATCTTTCAACTTCATTAGTAAAAATTCAAAACAATGCATTTGAAAATACAACAAATTTAAGCGTAGTTACTTTCCCTACAAAAATTACCTTAATTGATAATGGTGCATTTAAAAATAGTGGTTTAGTAACTCTTACAGTACCCGGAACTTTATCAAATGTTGAACCAGAAAGTTTTATGAATTGTAAAAAACTTACAACTGTAACTTTTGGAACAGGTTGAGATAATAAAGATATTAAATCTAAAGCTTTCTATGGTTGTGATGTGTTAACTACTGTAACATTTGCTTCAAAAGTTAAAAATATTTTAAAAGAAGCGTTCTTTGCTTGTCCTCTTTTAAAAGAAGTTTCTATACATAAAGGAACAGACTGAAAAACAGGACTTATGAACAAAAACAAAGCTTTTGATGAAACTATTACAACTGTTAAAGTAATTAATTAATAATTTTAGGACTTGCAAAATTTGCAAGTTCTTTTTTATGCTTATGTTTTCATAATTTTTAATTATGTGAAAAATTATTGTGCTTAAAAATTAAATTAAATATTAAAATATAAATAATTAAAAATTTAAGGAGAAATATGAAACGTACAATTTTAGTTGTTATTGATGGTTTAGGCTTACGTGAAGAAAAGCAAGGGAATGGTTTCAAGTTAGCTAAAACTCCAACATTTGATAAATTATTTAAGGAATATCCAAATAGCATTTTACAAGCTAGTGGTGAATATGTTGGCTTACCAAAAGGCCAAATGGGAAATTCTGAAGTAGGTCACTTAAACATTGGAGCTGGAACAGTTGTTTATACTGGTTTAAGCTTAATTAATAAAGCAATGAAAGATGGAGAATTTGAAAAAAACAAAACCTTCAATGAAGTTTTTGCAGATGTTAAAAAGAAAAATTCAACACTTCATTTAATGGGTCTTTTAAGTCCAGGCGGTGTGCATTCACTTGAAGATCACTTATTTAAATTAATTGATATGGCACATGCTTATGGACTTAAAAAAGTATCAGTTCATGCCTTTGGTGATGGTCGTGATGTAGCTCCAAAAAGTATTATCACTTCACTTAGAAAATTACAAACTCAAGTTGACAAATATGGCTATAAAATAGCCTCAATCGGCGGAAGATTCTATGGAATGGACCGTGACAAAATGTTTGATAGAGTTGAAAAACATTACGAAGCATTATTAGGAAATTCAGACCATACATATGAATCAGTTATTGATTATGTAAATAGCCAATATGATGAAGATATTACTGATGAATTCTTTATTCCAGCAATTGACAAAGGTGCAAACTTTGTTAAAGATAATGACTCAATTATTTTCTTTAACTTCCGTCCAGATAGGGCAAGACAATTAACTCATTTATTTATTGGTTCAAAACTTTATGACAATAAACCAAAACATCCAGTTAAAATTTCTAAATTCTGCTCAATGATGAAATATGAAGGAATTAACACTCTAGTAGCATTTAGTGAAATGGAAGTAAAAATGCCTATTGGTCGTGTGTTAGAAAAAGCAGGCAAAAGTCAATTACGTGTTGCAGAAACTCAAAAATATGCACACGTTACCTACTTTATGGATGGTGGAAACGATATTGAATTCAAAAATTCAAAACGAATCATGGTGCCTTCATTAAAGGTTGAATCTTATGCCAATGCTCCTCATATGTCAGCTGAAGGAATTACAGATGAATTAACTAAAAATGTTTTAGATTATGATGTAACAATTATGAACTATGCAAATCCTGACATGGTCGGTCATACAGGAAACTTAAAATCAACTATTGAAGCAGTTAGTTTCTTAGATACTCAAATTAAAAGACTTTGAGACTGAGCAAGTAAAAACGACGTAACTATTTTTATTACAGCTGATCATGGTAATGCAGAAATTACTGAAGATGAAAATGGTAAACCAGCTACAAAACATACAAGTAGTCCAGTTATGTTCATTTGTACAGATAAAAATATTAAACTTGCAAACGGTAAATTAGCTAATGTTGCACCAACAATTTTGGATTACATTGGTGTTAAACAACCAAAAGAAATGGATGAAAAATCATTATTAATTCGTAAATAAAACAAAAATTACGATTCTTTAAAAACATAAATATCATAAATTTGAAATGCTCTTTTTTTATAATGAGCATTTTTTATTTAAATTTAATTATTAAAGTTTTTTAAAAAAATAAATTAACTTTATAATTGAAATATGAAAAATAATAATAAGTTAATTAAAAGAGTTTGCTTTAGCGATGTTGATGGCACAATTTATGGATTTCCTAACAAAGAATTAAAAAATGAAGTAGTTAATTCAGTTAGAAAAGCAAAAGAAGAAAATGATGTCGAATTTGTTATTACAACAGGAAATCCAGCCAATGAAAAAATCAAAAAATTAGCTAAAATAATGGGCTCAAGATATATCTCAACTGCTGGTGGAGCGAGTGTTTTTGATCTTGAAAAAGATGACTATATTCACCTTGAATTAATGGATAAAAAAGAAGCTCAAAAGATCTTTGACTTGTCTAAAAAACATCCTGATTTTGTGCATTTATATTATTTTGGAAAACATCAATTTTATCTTTATAATCCAAGCAAAGAAATGGAAGAATTTCTTAGTGAATTTTGTGAATATTATGACTGAAATAAAAGTGGTGAAATTAACCAAGATCTCCATAAAATAGAGCTTTTTGGAACAAGCGATGAAATAAAAAAAGCTTATGAAGAACTTAAAAAATTAAAGTTAAAACTCAAAGTTATTTACTTTGGAACACACATCGAAATTACTCCAAAAGGTGTTGATAAAGGCTATGGTTTAAAATGAATTTGTGAAAACATTTTTAATTGCAATTTTGATTATGTTATGTCAATAGGCGACAGTGAAAACGACATTGCAATGTTCGAAGCTTCGGGCTATTCTTATGCTATGGATAATGCTAAAGAAATTGCTAAAAAAGCAGCTAAATATTACACAAGTGATGTCGAACAAAATGGTTTAGGTGAAGCTATAAATGATTATATTTTTAGAACACACTTATTATTAGAAAAACAAAATTTAGAGCAAAAATTAGATAAGCAAAAAGCAAAAAGAAAATACTAGAAATAGGCTATATTCAGCTTATTTTTTTAATATTAATATTAGATTGTAAAAAAATAAATTAAATAAGTAATCAAAATTGAGTTTTAATATATAATTTTGTTAAGCAAATTATATAATTTGTCTTATTTTAATTAAAATAAAAATTTAAGAAAGGTAATAGGTTACTTATGAATTATTACAACGATTTTGATATGAATCAACAACCTAAAGCGAAATTCTGAAGACCTAACGAATGAAATGCATCATTGGTGCAACCTAAGAAATGATGATACTTTGGCGTTATTGGACCAATTATGGCCTTAATGCTTGTCATGATCATTTTAGGTAGTTACTTCATTGCTCAACCTGATAATGGTTCAGGTAAAAATGTTGAAACATATATTTGTTTAATCTTCTTTGGTGTGTATGGTTTCATTATTACATTGCTATTAATGATTTACTTCTTAATCTTATGATTAGGCAAAAGTCAACAAGCATTTAAAGCTGCTAAAGTTTTCTTTATAATGACACTTATATTGTTCCCATTCTTGCTTATTTGATGTTCAGTTTTAAACTCAAGAATGGATCCACCAGAACCTGAAGAAGTATTTAATCCTTATTCAGCTGCTAATGGTTATGGATTTAATCCAGCATGAGCCTCACAAACTCGTACATTTGCCTTAACAGGCCAATATACAGGTTCTTATGCTGCTCAACCACAACCTCAACAAACACAACAATTTGGTCCACAACCAAATCCACAAGCAACTGGTATGTTTAATGTACCTCCAAGAGGCCCACAATTTGGTCCACAACCAAACCCTTATGCACAACAACAAAGTGGAATGTTCAATGTTCAACAAAGACCATGAATGTTTAATGTACCTCCAAGAGGCCCACAATTTGGTCCACAACCAAACCCTTATGCACAACAACAAAGTGGTATGTTTAATGTACCTCCAAGAGGTCCACAATTTGGTCCACAACCAAATCCATATGCACAACAACAAAGTGGTATGTTTAATGTACCTCCAAGAGGTCCACAATTTGGTCCACAACCAAATCCATATGCACAACAACAAAGTGGAATGTTTAATGCACAACCTCAAAATCCACAACCAAACCCATATGGTCAACCAAATCCACAAGATTCAGGTATGTTCCAAGATCCTAATGGTCAAGACTTCGGTGGTTTTGATCCTCAAGGAAACTAAAAATTTAAACAATATTAAAAAAACTTGCAGACGTGCAGGTTTTTTTAATTTGCCTATAAAATAGAGCTTTTAGAAAAACAAGCTTAATTAATTTTCACTATATAAAAAGTTAATAAACATTATTAATATTGATTTTTAAATATTAGTATAAAATTAAAGTATTATGGGTTTTAAATTTAAATCAACAGAAATGCTCATTGCAGAAAAAACATTGAAAAAAATTAATCAATTCGAGCCATTAATTTCTCGTTTAAGTGATGATGAACTTAAACAAAAAACACATCAATTTAAGGCTAGACTTGCAGATGGTGAAACTCTCGACAAAATGAGAGCAGAGGTTTTTGCTGTATGTCGTGAAGCTACAAAAAGAGTTTTAGGTAAAAGACCTTTTGATGTGCAAATGATTGGTGGTGTATTGCTTGATTTAAGTTCAATTGCTGAAATGAAAACTGGAGAAGGTAAAACTATTACTTCTATTGCTCCAGTATATTTAAATGCACTTAAAGGCAAGGGCGCAATCGTTTCGACAGTTAATGAATATCTTACTGAACGGGATGCTGAAGAAATAGGCCAAGTATTTAATTTCCTTGGAATGACTGTTGGTGTTAATAAAGCTCAAATGGATCCTTACTTAAAACGTAAAGCTTATGCTTGCGATATTACTTATTCTGTACACTCAGAATTAGGTTTTGATTATTTACGTGATAATATGGCAAAAACAATGGAAGACAAAGTACAACGCGGTTTACACTTTTGTTTAATTGACGAAGCTGACTCTATTCTTATTGATGAAGCTAAAACACCTCTTATTATTTCAGGCGGAGGTGCAACTGTAGATGGTAAGCCATCTAATTCAAATACTTATTATAGTGCTGATCAATTTGTTAGAACTTTAGATGACAAAGACTATGAAATTGATGAAGAAACAAAAGCTATTTCACTAACCTCAAGAGGTATTGAAAAAGCCAACAAATTCTTTAACTTTAAAAATCTTTATGATATTGAAAACTCAGAAATTGTTCATAGAGTTCAAAATGCTTTAAGAGCTCACAAGATTATGAAGAACAATGTTGAGTACATTGTTCGTGAAGGAAAAATTGAATTAGTTGATGCTTTTACTGGACGTGTTATGGAAGGCCGTGCCTATTCAGAAGGTCTTCAACAAGCACTACAAGCTAAAGAAATAGTTGAAATAGAATATGAAACTAGAACCTTTGCAACAATTACTTATCAAAACTTTTTCCGTATGTTTGATAAGCTTTGTGGAATGACTGGTACTGCTAAAACTGAAGAACAAGAATTCATTGACATTTACAACATGCGTGTAAATGTTGTTCCTACAAATAGACCAGTAATTCGTAAAGATTTAAGTGATTCTATTTATGCTTCATATGATGCAAAATGAAAAGCTGTTACAGAAAAAATTAAAGAGTTATATGAAATAGGACAACCTGTTTTAGTTGGTACTGCTCAAATTGAAGATAGTGAAATCTTGCACCAATATTTATACAAAGCTAATATTCCACATACTGTTTTAAATGCTAAGCAAAATGCTTCAGAAGCTGAAATTGTTTCTAAAGCCGGTCAAGTTAAAGCAGTTACAATTGCAACCAACATGGCAGGTCGTGGTACTGATATTAAACCAAGTCCTGAAGCTTTAGAATTAGGTGGACTTTATGTTATTGGAACAGATAGAGCAGAAAGCCGTAGAATTGACAACCAGCTACGTGGACGTAGTGGACGTCAAGGTGATCCAGGGATTTCAAAATTCTTTATTTCACTTGACGACCAACTTATGAGACGTTTCTCAAACTATGAAGAATTCAAAGAATCTTATGCTTTAGAAGGCGACAAAGAAATAACTTCAAAATCGTTGTTACATGGTTTTGAACAGGCTCAAAAGAAAATTGAAGGGTTCAACTATGATAGTCGTAAAAGTGTTTTACACTATGATGATGTTATCCGTCAACAACGTGATTTATTCTATGCCCAACGCGATTTAATTTTAATCAATGAAGATATCTCATTCATCGTTGAAAGAATGGTTCGTAAAACTGCAACTATGTTAAGTAACTATCCAATTTTTAAAGAAAAGAATGGTTTGTTTAAACACCAAGTATTCACTGATTACATTAATGATGTTTTTCTTGGTCATGGTACAAAAGAACGCTTGGACTATGAAGAAGTTAAAAAAATTTATGACTCAGAAATTAAAGATTTCTTAATTGAAAAATTAGTAAATTTCTATAAAAAATGAAGAGAACAAGCTATTCGAAATAGCAATGATGATACTGATTATATCAATTGATATGAAAAAGATTTAGTGCTTGAAATTGTTGATATTTATTGACAAAACCATATTGACACAATGGATAAATTGCGTTCAAATGTTAACTTAGTTCAATATTCTCAAAAGAATCCATACCAAGTTTATACAGATGAAGGTAGCAAGAAATTTGACCAAATGCTTGAAAACATTGCCTATGATGTAACTAAAAAAATCTTTGATGATCGTATAGGTATTCCTTCAATTATTCCTTATGAAGTTCAACAAGATGATTTATTTAAACAAATTAAAAATACAATTATTTTTGATGATTCTTTAACTCAAGAAGAACGTGAACAACAATTACTTGAAATGTACAACAGCATTAAAGGACAAATTGAACAACAAAAAGAAAATTAATGTATAATAATCATGAGCCGACATTGAGGTTAAAACTTTAATGGTGAAGTCCTAAAAAGGACTTTTTTTTATTGTTATTTAGACCAAAAATGCCAAAAATTGGCTAAAAATAAGCTTTTTATAACTTTTTTCAAGAATAGATAATGTTATAAAGTATAATAAAAATATGAATACACCTCAAGACAACAAAAATCATGAAGATAAAAGAGTACAAGTAGAAATAAAAAGATCAAAAGTGAAACAATCATTTTTGCATTTTGGCTCACTTTATCGTGTTAAAAAATTAAGATGACAAGTTCTTTTAAGTGTTTTAATTGGCTTAATTTTTGGTTTAGGACAATTCCTTTTAGTCCAAATAACAGGACTTTATAACTTAGGTTTAGATGCTTTCTGTCAATCAGTTGCTCGGATTGCAAAACACTTTATTACTGATGATAACTGAAAAAATAATATATATAACCTTTTATTCTGAACAGTTAACTTATTAGCTAATATTCCTTTATTTATATTAGGTTATAAAAAAGTTAATAAGCGTTTTGGGTTTTTAACAATAATTTTTATGACTACTGCTACTATTTTTGGGTTGATAATTGGTTTCATAACAGGTAGTGAAAATTGATTTATTTTTACAAAAATTAAAGCAGAAGAATTAACATGAGATGAGGCAAAAGGCGGCAATTTAACAATCTTTGTTTATGCATTAGTTTGAGCATTTTTACAAGCAGGAATAGCAGCGGCTTTATTAATAATTAACTCAAGTACAGGCGGAAGTGATGTCTTAGGAGTTTATTGATCACACAAAAAATTTAAAGATGTTGGTGCAAGTTTCATTTTTGTTCACTTAGCATTTTTAACAGTTGCTAATTTAATAGGAACTTATATTCCTTTAATTACGCCAAAAGGATCTTTTAGAGAAGGCGCTTATAAACCAGAATATTTCTTTAATCCTTCATTTATTGCTGGTATTGTTATGGTTTTAATTAATGGGGTTGTGTTAAATGTTCTATTCCCTAAATATAAAATTGTTAAAGTTGAAATTTATTCATCTAAAATTCCTGAAATTCAAGAAAAAATTAATACTTTAAAAGATGTGAGATTTGCAACAAGCCACTTAAGTGTTAAAGGTGGCTACTCAGGTCAAGAACAAGAAGTTTTAGTTACAATGTGTTTATATTTAGATGCAGCACATCTTCTTGAAATTGTTCGAGAATTTGATGAAAATGCCTTATTTACAGTTACAGACCTTAAAAAGGTAGATGGATATTTCTATGTTTCAGTAGAAAATCACAAGAGTTTATTTAATAAAAAAATTGAAAATAAAGAACAAAAGAAAACTAAAGAAAATGTAGAAGATAACTCGAAAAAATAGTTGTCTTTTATTTTTTAAATTTTAGTATTACAATTATTTTTTTAAATTATAATTTTTTATTATGGAAAATAAAAATAATCAAAATGAATTAACAAACCAAAACGAAATAGAGATTCAACGTTCAAAAATTAAACAGTCGTTTTTGCATTTTGGTACTTTATATAGGGTTAGAAGTTTTAAAAAACAAATAATTTACACCATTATTATTGGAGTTCTTTTTGGTATAGTTCAACATTTACTTATTTCAATAACAGGACTTTATAATATGGGTTTAGACGGACTTTGTCAGTCAATTGCTCGGATAGTTAGACACTTTTTAAGATGAACAAGTTTAGTATGAAAAGATAGTGACAAACTGATTGAGTCCTTCTGAGTTTTAGTGATTTATAATGTGTTATTCTGGCTGTTAATATTAGTAGCTAATATTCCTTTATTTGTTTTAGCATATAAAAAAATTAGCAAAAAATTTGGTTTCTTATCTATTATTTTCTTAGTTGTAGCTTGTATTACAGGTTTTTGTATTGGTTTAATTCCAACCTCTGAGCAATGATTTATGATTTCAAAAATTGATTCAGCTAATTTAACTTGAGAAACAGGTAAATTTAAAGATTTTTCAATTTTCTTATATGCAATACTATGAGCATTTTTACAAGGAGTATTTGCTTCAGCTTTATTAATTATTGGTTGTTGTACAGGAGGAGCTGATATTCTAGGAGTTTATTGATCACACAAAAAATTCAAGGATATTGGTGCAAGTTTTATACTTGTTCACTTGTTATTTTTATTTGCATGTAATTTAATTGGTAGTTATATTCCTTTTGCAGTTGAAGATCCTATTGCAGCTTCTTTTTCAAAACATCCAGGTAATAGATATGAATTAGAAAACTTTTTCAACCCTTCATTTGTCGCCGGATTTATTATGGTTGTAGTTAATGGTATTGTTTTAAATGTTTTATTTCCTAAATTTAAAATGGTTAAAGTTGAAATTATTTCAAATCAAGTTGAAATGATTCAACAAAAATTGAATACTTTAAAAGACCGTCGTTATGCTACAAGTACCTTAACTGTTATTGGTGGTTACAGTCATAAAGAACAAAAAATTCTTTTGACAACTTGCTTATATATTGATGCAGCTAAATTATTAGAAACAGTTCGTGGAATTGACCAAAATGCCTTATTTACAGTTATGGATCTTAAAAAAGCAGATGGTTATATTTATACAACTCACGAACATCAAAATCATTTATTTAAACATAAAAAACGTGACGAAATTAATGATCAACAAAAGCAAAATTAAAAATCGGGAACCCCGATTTTTATTATTAAATTTTATTATTTTTTGCCTTTGTGGCTAATAGCATCTCTAACACGGCCTGTTGTTTTGTGAATCAATACAGAACCACCACGTTTTTTAGCAATTTCATTTGCTAATTCAACAGCTTCTTTTTGAGTTTTAACAATTTTTGTAGGTCTTGAGTTCCCAACACCTTTAACTTGTCAACCTAAGCCTTTAGGAGTTACGTGGTATACTGTTGCTTCTTTGTTTTTTGTTTCTGCCATAATTTTCCTTTCATTGTTTTTATTAAGTGTATTAATATTATAAAGAAAAAAACAACAAATTTAAAATATTTTTTTAAAGAGCATTTTTTCCAATAAGCCTATTTTAAAAAGCTATAAGTCACAATTTATTTTTGAACATAAAACCTAATTTTTTAAACTAGTCTTCAATTTTAGCTAAGAAGTCTTTAGATTTTACATTATTTTCTTTAATTGAAAATATAATGTTGACTTTGCCATTTTTATCAAGAACTGTATATAAAAATCCATGAGAACCTGCATTTTTACCTGTAATTTGTTTTGAAGGATGTGTTATAACTTGAGATGGTTTTTTCAATCCAGTTTTTTTGTAAGCTTCAATTGCTTCTTTTGTTAATTTAAGCGAAGTTAGATTAATTGGTTTTTCCTTTTTATTATTACCTAATTTTTTTAAAGTAAAAGTATAATTTTTTGGATTAATATCAATTCTCATGTTTTTGTTGTTCATGTATTCTTTAATTTTAGCTAATTGAGTAGCATATCCAGGATCAGTTTTGTCATAGGTGAATATTTTGCCTTCTTCTGCCAGCTTTCTTAAATCTTCATAAGGATTTGCTGGAACTTTACCTTGTAATTCAACATGAGTAAAGTATTCTTTATTAATGTTTGTATTTTTGTAAGTTACTTTAACTCTTAATTTTCCTGCTTTTTGTAAAGCAAGTTTTTCGGCATTATTTTTTGGTCAATCAACAGTTACGCCAGTTAATGTGAAAATGTCATTTGTTGTTGTAGGTACATTTAATTTAGTTTTTTCATAGATGATGTCATTATCTGTAGCCTTTGTAACATCACAAGGGTTAGGTAATGAAAGATTAATTTCAGCTAAAAATGTATCTTTTAATTCTACAAATGATTTTTCTGCAAAAGTTAATTCAAATTTCTTTTCTTTTGGAGTTGATTTAGTTGTGTATGAAACCGTAATAGTTAATTTACGTGTTGCATCATCAAAATTTAATGTCATTAAATTATTTAAATTAATTGTTGTGGCTTGAATATTCAATTTTTCAAGATTTTCTTTATTGAATTCTTTTTCTTCATTTGTATCTGGATCAGTGTAGTAAAGTTTTACTAAATTTTGTTCTTCTTTAATTTCTGAATAATAGTTCTTTTAACTAATTTTAGTTTTTAAAGTACCGATTAAGTTTTCAAATTTGTCTTCTGAATATGGAGCTGGTTTTTTGAAATCATTAATTTCATATTCAACTTCAACATTTTCTTGACTTTTATCTTCAAAACTTAAAGTTAATTTCAATTTTGCTTGACCTAAATTATTATTAGCAACAATTAATTCTGTTTTATTAATTGTAATCTTTTCTTCACTTAGATATTCGCTTAAGGTTTTACCACTTGGAGTTGTGGCTGTAACTTGAGCCAATAAATCACTTGTGTGAGCTGCAATATATTCTTCAATTGTTTTACTTGCTTTTTCATTTAAGTCAACATTTGTTAATTTAGCCATTTTTTGTTGAATGGTTTCTTTTGTAACAAAACCAGTTATTTCTTTGTTGTATGCATATTCTGGTTGATTTCATTGTTGTCATTTTGCAACTAATTTAACTTTTCCTTCAAAATTGTCAACAACTTCTAATTGATATTTAATGTAATTTAAACCTTCCATGTTTTTAAATACAGATAAATCATTTTGATAATCTGCATTTGTGAATGTAATTTTTTCTTCTTTTTCTTGATCAGTTAGTGCATTAAATTCTTCAACATATTCTTGTGCAGTTTTAGTTGATTTATCTTTTAAATCCACATTAAATGCTGCTTCTAATTCACTATTAACTTTAATTTCTTTAAAGCCTGCAACTACTTGACTTCAAACATATGTTTCAGTTTTAGTGGCGTCTTTTCAACCAAAAGTAATATTAATTGATAATCATCCTGAATAAGGTTCAGTTTCACCTGATGAGGTATCAGCTTCTTTATTTTTAATTTCAATACCTTTTTCATTAAATACTTTTGTTACATCAACTTGTGTTTTATTTTTGAAGTATCTTAGATCTGTAATTGTATAGTTGAAAACATTGAAATCTTTTTTGTTTTCTAAAACATCAATAGTATCAAAAATACCTTCTAATGTTGCTTGTAACGATTCAGTTTCTTTTTTGAATCCAGTTAATTTGATTGTAATTTCTTTTGATTTAGTTTTTGCTTTATTTTCAAAAGTTGCTTTAATATTTGCTTCACCTTTTGAAGCACTTACTTCTTCTATTTCTATGCTTTTAATTGATATTTCATATTCTTTTAAGAAAGTAGCTAAATCTTTGCTATTTGAAGTTGCTATTGACAATTTAGCTTGTAATTTAGCTAGATCATTAAATTGATTTTTGTATTCTTTAACTGTGTATTTGTCTTTTTCTGCTAAGTCCAAACCAGTTATAAATTTGATGTTTTCGTCAAATTTGTCAGTATCTACATAAAATCCTACAACCTTAATTGTGAATTTTTTAATTTCTTTATTTCCTTCTTTTTGAGTAAGGAAAATTTCTAATTCACCCTTAGCTTTATCTGCTTTTAAGTTGTCAATTATATATGTATATTTCTTTGTTGTACTTTCAAATTTAATATCTTCTTTTTTAATTTGATCAGCTTCTTTTGTAGTTGTGCTAATCTTATATACTTTGTTTGTAGAGTCATAATCAGCATTCTTAATATCTGCAATAGACAAAAGTTTAAAGTCTTCATCTGCTTTAATTTCTTCTTTTGTTGTGTTTTGACATTGAGATGAAATTAAAGGTAAAACACCTGCTGTTACGCTTGAAAAAATAAGACTTAATTTAATTTTCTTCTTCATATATTTCTCCTTCATAAGAATAAATTCTTATGCTAATAAATTATCTTAAATTCCTATTATTTTTTAAATAAATGGAAAAAATATCCACATTTTTCAAAATTAATGAAATTAATAAATTTCTCATTTTTTAAAGGAATTTACCAAAAACACTATAAAACAGCATTATTGGATAAAAGCGGTATAAATTGTGAAAATTTTATGTGGAATAACTATGTTAAAATTACTTTTTTTGTTACCAAAAATAATATAATTCTATAGCAATAAATTTTATAAGAGAGGTGCAAATGACAAGAAAAACTAAAATAGGTATTTTAATTGGAACAAATATTATTGTTACTGCTGGACTAATAACAGCTTTAGCAATAGCGCCAACTAGAAATAATAGTAATCATTCAAATTCAGGCACAACAACAAGTGAAACAAGAAAAATAGAAGTCAAAGCTAAAGTGAGTGTTGATTTAAGCAAAAAAATTGATCAGTTATCTAATCCAAGCACTGAGTGATTTGATTTTAATGGAAAAGCAATTCAAGAAAGAGTTGTTAAATTAACTGGAAAATATGATTCTAAAACTCAAATTTTAACCTTAGAATTTGAGTTATTAGATACAAAAACTTCAAAATCACAACATTTTACTGCACAAATTAAAGTTGAAAATTCTGTAGGTGGAGGTACATCAACAAGCGAAACAAGAAAAATAGAAGTCAAAGCTAAAGCAAGTGTTGATTTAAGCAAAAAAATTGATCAGTTATCTAATCCAAGCACTGAGTGATTTGATTTTAATGGAAAATCAAGTCAAGAAAGAGTTACTAATTTAATTGGAAAATATGATTCTAAAACTCAAATTTTAACCTTAGAATTTGACTTATTAGATATCAAAACTTCAAAATCACAACATTTTACTGCACAAATTAAAGTTGAAAATTCTGGAGGCGGAGGAACAACAACAGGTGACTATGTTTATGATAAAAGTAATAATTATTATTCTTCTTTAGAAGGTTTAAGAGGAGTTGATTTATTTAATGCTTTATTAAATTTACAAAAACAAAAAAGAAGCAAACTTACAAATTATGCAGATTTAGTTAATAGAATTTATAGAAATGCATTTATAGATAAATATTTCGAAAAAGACAACACTATTCTTGATGTATATTCAGAAAATCCAAATGGAAAAGACCCTTATGTTTTTAAAGTTGATGAATATGATGGAAGAGGCGGAAAATCAACAGGAAAATCATATGGTAATGGAGAAGGATCTATGTTTAATCGAGAACATATGATTCCTCAGTCATGATTTAATAAAGATGAGGCAACAAGATGTGATGCTCATTTTGTTTGACCTACAGATAAAAATGTAAATGCTTGAAGAGGTAATGATCCTCATGATAATATTAGTAGTCCATCTTGAACTTCTTTAAATGGAACTAAAGGGAAAGATGGTGTTGGTTGTGAACCTATTGATTACTTTAAAGGAGATATAGCAAGAGCATATTTCTATTTCAATGTTACACACCATAATGGAACACATAATAAAGGTGAAAGAGTATATACTTCAACTTTTCCTTTCTTTGTAAAACACTATTTAGATGTATATTCTAAATGAGCAACAAATGATCAAGTTGACTTAATCGAAATTGACAGAAACAATAAATTAGATGAAATCTACGGTGGTTTAAGAAATCCATTTATTGATTATCCTGATTTAATTGAATTAATTTGAGGCAATGATACAACTAAAAAATTTGAAAATCGTGGTGTAATCGTTAGTGTAAAAACACCTTAAAATAATATAAATTCAACAAGCAAGAAATAATTTGCTTGTTTTATTTTTATCTAAAAAGCCTATAAAATAGTTGATTAGAGTAATAACACAAAAAATTTATTTTAATTAATTAAAATACTAAATTAAATCTTGCTATTTGAGTTTATAATTAAAATATGTCAATTTTTAAATTAATTTCACCTTATAAACCTGCAGGTGATCAACCTGAAGCAATTAAAGAACTTGTAGAAAATATTAAAGACAATATGCAACATCAAGTGCTTAAAGGAGTTACTGGTAGTGGTAAAACTTTTACTATTGCAAATGTTATTAAAGAATTTGATCGCCCTGTTTTAGTTTTAAGTCACAACAAAACTTTAGCTTCTCAACTTTACAGTGAGTTGAAAGGTTTCTTTCCTCATAATCGCGTTGAGTATTATGTTTCATATTTTGACTACTATCGACCCGAAGCTTATATTCCTTCTTCAGATACTTACATTGATAAACAATCTCAAAATAACAAAGATTTAGATGCTATGAGAATGTCAGCTATGAATGCTGTTTTAACTAGAAATGACACTATTATTGTTGCTTCAGTTTCGGCAATTTATGGTGCACTTAATCCTAGTGAATATTATGCTTCAGTTTTTCCTATTGAAGTAGGTATGCAAATTAAAAGGCAAGAATTCTTTAAAAAACTTGTTCAAAGAAACTATAAAAGAAACCAAATTGATTTAGAATTAGGCACTTTTAGCGCCAAAGGTGATGCAGTTTATATTCAACCAGCTGATAGTGATGAATATCAAATTCGAGTCGATTTTTTTGGCGATGAAATTGATTCAATTGCAACTTGCGATCCTATTACTAAAGAAGTAATAGAACGTTTTAGTCATTACACAATTTTCCCCGGAGATGCTTATACAGTTGATAGAAGTGTTATTGATGAAGCTTGTGACAAAATCTTAGTTGAATTAGATGAGAGAATTAAATATTTTAAAGCTAATAACCAATTAATTGAAGCTCAAAGAATCCAAGAAAGAGTTGAAAAAGATGTCGATAGCTTAAGAGAATTTGGTATGTGTCCTGGTATTGAAAACTATGCAAGATATATGGATAGAAGAGAAGCAGGCGAGCGTCCTTATACCTTATTAGACTATTTTAAAGGCAAAAAACCACTTTTATTTATTGATGAGAGTCACATGATGATTCCACAATTAAATGGAATGTATCATGGTGACTACTCACGTAAAAAAAACTTAGTTGATTATGGTTTTAGATTACCAAGTGCACTAGATAATAGACCACTGCTTTTTAAAGAATTTGAAAATGAATTTGATTTTCAAACTGTTTATATTTCAGCTACCCCTGATGATTATGAACTTGATAAAACTCATGGTTTATTAACCAAATTATTTGTTCGTCCTACTGGTCTTTTAGATCCTAAAATTGAAATTAGACTTTCTAAAAACCAAATTGAAGATATTTATGATGAATTGCAAAGACAAAAAGATAAAAATGAAAGAACTTTGATTTTAACTACAACTAAACGTTTAGCTGAAGAATTAAGTAGATACTTTTTAGAAAAAAATGAAAAAATTGCTTATATCCACTCAGAACATAATACTTTTGAACGGAATGAAATCTTAAGAAAACTTCGTAAAGGCGTTTACGACACTGTTATTGGAATTAACTTATTGCGTGAAGGTGTTGATTTACCTGAAGTAAGTTTAATTATGGTTTTAGATGCTGATAAAGAAGGCTTTATGCGTTCGACTAAAAGCTTAATCCAAATTTCAGGTCGGGCAGCTAGAAATGCTAATGGTCGAGTAATTTTCTATGCAGATAAAACTACTAAAAGTATGCAAGAGTGTATTGAAGATAATGAAATTAAAAGAAAGATGCAGATTGCTTATAATAAGAAACATAATATTACACCTAAAACCATTATTAAACCAATTGCTGAGCCAATTCATGGCGAAGGAATTGAAGGCGAAATTGAATTCTTTTTCAAAAACGAAAAACGAAATCAAACTCAAAGTGGCAAGAAAGCTAAAGAAGAACTTATTAAAAGATTACGTGAACAAATGAATCAAGCAGCTAAAGAATTGGACTATGAAAGAGCTATTGAATTGCGCGATATTATCCTAGAATTACAAGCTGGTAAAGATAAGAAAGTTGAGGAATAAAAATGAGAGTGACATTATTTGATTTAGTTAACAACTTAAAGTATTATTCTTCTTTAACTATTAACTTATATTCTATTAATAAAGCTATTAGACAACTATTAAAAATGATTCCAAATTTGAGTGCTAAAGAAAAAGAACTTTTAACTTTAGATTATTTAGGAACCGAGTTTAATAAATATTCGAGTGAGCTTTATAAACATGCAATTTGTATTGTTTATTTAGCTAATAAATATAAAGTTAATTGAGCGGTAAATTTTATAAAAGACTTAAAATTAGAATTAAATAAAGTTAATGATATTGAAGGCTTTTTAGATTTAGTTTTAACTCAAAAAAATCAAAAACTAATTAAACAAAAATTAAGTCAAATATTCAAATTTGTTAACTTAGATGGCAATGTAAAAGACATTATTATAATTTACGATTTAATCTGTTTTATAGGTTTATTAGTAAATTTAACAAACACAGAATTTGAACTTTTACCATATGATGCATGTAATATTATTTCGTAAATAAGGAGGTTTTTTATGTCAGCCAAAGAAGAAATTATAGTTAAAGGTGCTAGAGAACATAATTTAAAAAATGTTTCAGTAACTTTACCTAAAAATAAACTCATAGTTTTTACAGGGCTTTCAGGTAGTGGAAAGTCTTCTTTAGCATTCAATACTATTTATGAAGAAGGTAGAAGAAAATATGTTGATAGTTTAAGTAACTATGCTCGTTTATTTTTAGGTGGAACAAGTAAACCTGATGTTGATAGTATTGATGGGCTTAGTCCTTCTATTTCAATTGAACAAAAAACAACACATAATAACCCTCGTTCAACTGTTGGTACTGTTACTGAAATTTATGACTATTTTAGATTACTTTTTGCTCGTATTGGTAAACCATTTTGTCCTCACCATAATATTGAAATAACTAGTCAAACCAATAAAGATATTATTGATTCAGTTTTCAATTTAAAAGATGGATCAAAACTTTATATTTTAGCTCCTGTTGTTGATGGTGAAAAAGGAACCTGAGCAACGCTTTTAGAAAAACTTAAAAAAGAAGGATTCTTAAGAGTTAAAGTTGATGGTGAAATAAAAAACCTTGAAGATGAAATTAAATTAGAAAAAAATATTAGACATACTATTGATATAGTAATTGACCGTGTGGTTTTAAATGAAGAAAATAGAAACAGAATTTCTGAAGCTTTAAGTGTTGCTGTGGAATATGGAAAAGGCATAGTTAAAGTTGAAACTACTGATGATGAAGTTAAAACATTTTCAAAATTACACTCTTGTGTTTATAAAGATTTTGAAATGCCTAAAATTGAAACTAAACTTTTCTCATTTAACTCGCCTTATGGTATGTGTCAAAATTGCAAAGGACTTGGAGTCGATTTAAAAGCTGACTTTGATGCTCTAACTCCAGAACCTTGAAGAAGTATATTTGAAGGTGGAATTAAATATTTTGAAAACACAGTCAATACTCAAAATTTAGAGTGACAAGAATTTGAAGTTTTATTAGATCATTACAAAATTGATAAAAATTGTCCAATTGAAGATTTAGGTAAAAAAGACTTAGAAATCATTAAATATGGATCAAAAGAAGAACTCGACTTTGTTGTTGTTTCTGAAAGTGGAAATAAATGAAGAAAACACAAAGTTATCGAAGGTATTTTGACTAAAGTCCAAAGACTTTATATAGAAACAACTAGCGAAAGAATTCGTGACTGACTTAAAAAATACATGGGTACTTTTGAATGTTCAACTTGCCATGGAAGTAGATTAAATGAATATGCACTAGCAGTTAGAATTAATGGTTATAACATAGAAGACTTTAGTCGTATGTCAGTTGAAGATGCTTTGATTACTATCGAAAACTTAAAATTGAATGAAGAAGAGACTAAGATTTCTACTTTAATTATTAATGAATTAATTAACCGTTTAAGTTTTCTTAAAAATGTTGGACTTAACTATTTAACTTTAAATAGAAATGCCGAAACTTTAAGTGGTGGTGAAAGTCAAAGAATTAAATTAGCAACTCAAATTGGTTCTAATTTAACTGGTGTTTTATATGTTTTAGATGAACCATCAATTGGGCTTCATCAAAAAGATAATGACCGTTTAATTAAAACACTTAAAAGTATGGTTGAAATAGGTAATACTTTAATTGTTGTTGAACATGATGAAGAAACAATTAGATCAGCAGATTATATTGTTGATATTGGACCTAAGGCAGGAATTCATGGTGGTGAAATTATTGCTCAAGGAACTTTAGAAGACATTATTAAAGAACCAAAAAGTATCACTGGAAAATATTTAAGTGGTGAAATGAAAATCGAAGTACCAAGAGTTAGAAGAAGTGGAAATGGCAAAGTAGTTACTATTTTTCAAGCTGCAGAAAACAACTTAAAAAATATTGATGTCAAGTTTCCTTTAGGTAAATTTATAGGAGTTACAGGCTTAAGTGGCAGTGGTAAAAGTAGTCTTGTAAATGAAATCCTAGTTAAAGGTATCGAAAGCGTATTAGGCCATAAAACAGGCAAAATCGGAAAATTCAAGAGTATTAGTGGACTAAATCAAATTGATAAAATTGTGCCAATTAGCCAAAGCCCTATTGGCAGAACTCCTCGTTCTAATCCAGCAACTTATACCTCAGTTTTTGATGATATTAGAGAAATTTTTGCCTCAGTTGAAGAATCACGTGCTAGAGCTTATAGCCGTTCTCGTTTTAGTTTCAATGTGCCTGGCGGACGTTGTGAAAAATGTCAAGGAGATGGTTCAATTAAAATTGAAATGCACTTTTTACCTGATGTTTATGTACCTTGTGACGAGTGCGATGGTCAAAGATATAATCGTGAAACTTTAGAGATTAAATACAAAAATAAAAATATAGCTCAAGTCTTAGATATGACTGCTGATGAAGCACTTGAATTCTTTAGTACTAGAGCAAATATTAGAATTAAATTAGAAACTTTAGTTGCTGTTGGATTAGGTTATATTAAGCTTGGACAATCATCAACAACACTTAGTGGTGGCGAAGCTCAACGGGTAAAATTAGCTACTTATTTACAAAAGAAGGCGACAGGAAAAACCATTTATGTATTGGATGAACCTACTACAGGATTACATTCTTATGATGTTGCAAACTTATTAAAAGTTTTAAATAAAATTGTTGATAATGGTGACACAGTTGTTGTTATTGAACACAATTTAGATGTCATTAAATGTTGTGATTATATTATCGATCTTGGCCCTGAAGGTGGGGTAAATGGTGGCCGAGTTGTGGCTACTGGAACTCCTGAACAAGTGGCAGAAAATGAAAATAGTTACACAGGACAATACCTAAAAATGATTTTATAAATCACAATTTTGAAGAAGCACAAGCTGCTTTTTTTATTTTTCTAATTTGCTAAATTTTGACCAATTAACCTATAAAAAAGCCTTAAAATTCAAAAAATAAATTTTAATTTTTCTTAATTTAAGAAGGCTTGAAAAATTTTGAAAAAATAATACATTTTTTTTCTTGGTATTTTAGATTTTATTGGTAAAATATAAACACATTTGCTTTTTTATTTAATATTAAATATTTATTATGCAAAAAACCTTTTTATGGTATAAGCTGACAAAAAAGCAAATTTTATTATGAGAAAAAAGGAGTATTACTGTATGGATGTTGCAACAAAAAAATATGTAAATAAATACCCTTACAAAGTTCGTAAATATGGACCTATTACCGAACGCCGTGATTACTCTGTAACTAAAATAACTTGAAACGTTCCTGACTTTTTAGTTACAAGTAAAGAATCATTCGAATGATTCAAAAAAACTGGTATTGAAGAATCTTTAAGAGAACACTACCCAATTACAGCGACAAACAAAAAAGTTTCATTAGAATATATTCCAAATTCTGCAGATTTGGAAATTCCTTCTAAAGAATATGAAGCTATTAGTGAAGCTAGAGTTAAAGGCACAAACTATGCTGCTAAACTTTATGCTAAATTTAGAGCTACAGCTACTGAAACAGGTATCGTTAAAGATGACAAAGTTCTTTTAGGTGAAATTCCACTTATGACTTCAGGTTGAAGTTTTATCATCAATGGTAGCGAAAAAGTTATCGTTAGTCAATTAATTAGATCACCTGGAGCTTACTTTGGACGTGGCGTTAGAAATAAACAATCTGATGACCTTTTCAACAAGGTAGAAATTTTACCTCGTATTGGTTCTTGGGTTGAAATCTCTCACAAGGTTACAACTAAAACACCAGATAGCGTAAAAATTAAAATCGATAAAAACAAAAATGTTAACTTAGTAACTTTCTTAGGTTCACTTGGTTTAGATCGCCAAATGATTATGAATCTTTTTGGTAAATCAGATGTTTTAAAAGAAACCATCAAAAAAGACAAAAAAGTTGATCCTGAGCTTGACCAAGACACAGTTATCAAAAACTGTCAAGAAGAATTATTTAGAGTTCTTCGTCGTGGTGACCGTATCACAGATGAATCAATTGCTAACTTGATTCCAAGCTTACTTTTCCACAGAAAACGTTACAGCTTAAGTGAAACTGGTCGTTATATGTTAAACAAAAAACTTAACTTAGTTGACCGTATTTCAGATACATTTTTAGGTGAAGACTTAGTTGTTATTAACAAAAATGGTGATGAAGAAAAAATTAAAAAAGGTACTTTGATTACACACAAAATTGCTTTATTAATTCAAAGCAACTTTGACAGTGGTAAATTACCTTCTGTTGAAATCGAAGGTGTAAGACCTGAAGTAGCATATGCTAAATTATTAAGTGATGCACAAAACAAATCACTTAAAAACAGAAACAAAATCATTATTTTAAAAATCTATCCAACCAAAAAATGAATGGATAAAGGTTTAACTCCAGTAAAAGTTATTGGAAATGACCCTAAATCAGTTGAACAACACTTATTAGTATCAGATATTATTGCTGCTGTAGGTTACTACTTCAACTTATTAGATGGCATTGGACAAGATGATGATCCTGACTCATTAACAAACAAACGTATTGTTGCAGTTGGTGAATTATTACAAAGCCAATTAAATGTCGGTTTAGTAAAACTTGAAAAAACTACACGTGAAAGAATGGGCGCTAAAGAACCTGACAAAGTAACACCTAAAAATGTTACAAACAATAAACTCATTTCAACACAAATGAAAACATTCTTTAACTCATCAAAACTTTCACAATTTATGGATCAAATTAATCCACTTGCTGAAATTTCAAATGAACGTCGTATCACTTCTCTAGGACCTGGTGGTCTTAACCGTGATACAGCTCAATTCGAAGTTCGTGACGTTCATGCAACTCACTATGGAAGAATTTGCCCTATTGAAACTCCTGAAGGACCTAACATTGGTCTTATCTTAAACTTTGCAACATATGCACAAGTTAATAAATATGGTTTTTTGCAAACTCCTTACTATAAAGTTGAAAATGGTGTAGTTGACTATTCAGAAGTTCATTATTTAACAGCTGCTGAAGAAATTGGATTTAATATTGCTCAATCAATTGTTAAAGTTGATGAAAACAATAAATTAGTTGATAAACAACTTACAATGCGTCACAATTACAACTATATTATTGGTAAACCTAAAGAAGTAGACTACATCGAAGTAGCTCCAAACCAAATGGTTTCAGTTGCTGCCGGATGTATTCCTTTCTTAGAAAATGACGATGCTAACCGTGCACTTATGGGTTCTAACATGCAACGTCAAGCAGTTCCTCTTTTAATTCCTGAAGCTCCTTTTGTTGCCACAGGTATTGAATCTGATATTGCTAAATATTCATCAGGTAACTTCGTTGCTAAAAATGATGGGCTTGTTGAATATGTTGATGGTAAGAAAATCAAAGTTAGAAATCAAAAAGGCACTTTAGATACTTACTATCTTAAAAACTTCCAACGTTCAAACCAAGATACAGTTGTGCACCAAAGACCTTTAGTTAAAGAAGGCCAAGAAATCAAAAAAGGTGATTTACTTGTTGATGGCTCAAGTTTCAAAAATGGTGAATTAGCATTAGGCAAAAACGTAGTTGTTGCCTTCACAACATATAAAGGATACAACTATGAGGATGCTGTTATTCTTAATGAACGTTTAGTTAAAGATGATGTCTTTACTTCAATTCATATTGAAGAACAAACAATTCAATTCAGAACTTCAAAAGCTGGAGATGATGAATTAACTTTAGATATTCCTAACGTTTCTAAATATGCAACACGTCATTTAGATGAACATGGAATTGTAAGAATTGGATCAGAAGTTGTTCCTGGAGATGTTTTAGTTGGTCGTGTAAGCCCTAAAGGTGATGACAACCCATCTCAAGAAGAAAAACTTCTTTCAGCTGTGCTTCAACAAAGACAATTAAATGTTAAAGATACTTCATTAAAAGTTAAAAATGGACACAATGGTACCGTTATTGGTGTTGATGTTTTAAGCCGTGAAAATGGTGATGCGTTAGAAGATGGAATTGAAAAAATCGTTAAGGTTTCAATTGCTATTAAACGTAAAATCAAAGTTGGTGACAAAATGTCAGGGCGTCACGGTAACAAAGGTGTTGTTTCAATCGTATTACCTGAAGAAGATATGCCTTATTTAGAAGATGGTACACCAGTTGATGTTATGCTTAACCCTCAAGGTGTTCCTTCACGTATGAACATTGGTCAAGTTCTTGAAATTCACTTAGGTATGGCTGCTAAAAAACTTGGATGTAAATTTGTAACTCCTACTTTTGATGGGGTTAAAAAAGAAGAAATTCAAGATGTTACTTTAGAAGCTGGTTTACCTTTAAGTGGAAAACAATGATTAATTGATCCTATTACAGGTAACAAATTAGATAAACCAGTTTCAGTTGGTGTTATGTACATGCTTAAACTTAACCACATGGTTGATGACAAGATGCACTCACGTTCAGTTGGACCTTACTCACTTATTACTCAACAACCTCTTGGAGGTAAGAGTCAAAACGGTGGTCAAAGATTTGGTGAAATGGAAACATGAGCTATTGAATCTTATGGTGCAACAAATGTTCTTCAAGAAATCTTGACATACAAATCAGATGACATTAATGGACGTAACCAACTATATGCTGCATTAGCTTCAGGACGTGAATTACCTAAACCAGGTGTCCCTGAATCATTTAACGTATTAAGCTATGAATTAAAGGGATTGGGAATGAAACTTGATCTTAAAAAAGTAAAACAAGACGATAATGATTTCCAACAACACTTCGACATTAGAGGAGGAGATTCAAATGAGTAACTTTATAGACAAAAACAAGTTAAATAAAGATCGTGAAATTGAAAAAATTACTCTTTCTTTAGCTACTAAAGATGACGTTCTTCAATGATCAAATGGTGAAGTAACTAAACCAGAAACAATTAACTATAAAAGTTACAAACCAGAACGAGATGGTCTTTTTGATGAATTAATTTTTGGACCAACAACTGACAACAAATGTCCTGTTTGTGGTACTAAATATAAAAGAAGTGATGAAGGAACTCAATGTTCAAAAACTCCTTTATGTGAAAAATCAAAGGCTACAATTTTAGCTAAAATTTCACGTCGTAGCCGTATGGGTCACATTCATTTACAAAATCCTGTTGTTCACTTTTGATTCTTCAAAATTGATCACTCAATTATTTCTAAATTACTTGGTTTAAGAGTTGCAAAAAGTTCTAAAACAGTTTCAAAAACAGATTTAGAAAAACTTATTTACTACAAAAGCCACATTGTTTTAGAAAGTGGTGGCATTAAAGCTCTTCAAAAGAATGCAATTATTGATATTGCTGAAGCTGCAGTTATTTATCGTGATGCTTTACTTGAAATTAGAAGCAAATATTCTGAAGAATCAGAAGAATATGAAATTATTAATGAATCACTAGAAGAATTAGAATCATATGCAACTAGTAAAATGGGTAAAGATTATGGTATCGACTTCTATGAATACAATGAAATTATTCACGAATACAGTGATGCTATTATCTCAACAGGTTCTAAAGCTATTGAATACTTATTAGAACATGTTGACTTACAAAAAGAAGCTCAATTAATTGAAGATGAAATTAAGAAAATCAATCGTTCAATTAAAGATAAAAATCACACAGGCACAAGAGGTCAAGAACGTTCAAAACTTTACAAACGTCTAGCTGTAATTAATGCCTTCATTAACTCAGGTCAAGATCCAAAAAGCATGCTTATTTATGATCTTCCAGTTATTCCAGCTGACTTACGTCCACTTGTTCAATTAGATGGTGGCCGTCACTCAACTAGTGATATTAATGAGCTTTATCGTCGTATTATTATTAGAAACAATCGTCTTAAAAAATGAGAAGAAACCGATGCTCCTATGCTTATTAAGCAAAATGAATATCGTATGATTCAAGAAGCGGTTGACTCATTAATTGATAATGCACGTAAAAAACCAAATCCTGTTACTTCAAAAGATAGCCGTCCTCTTAAATCAATTTCAGACGCATTAACAGGTAAAAAAGGTCGTTTTCGTCAAAACTTACTTGGTAAACGTGTTGACTATTCAGGTCGTTCAGTTATTGTTGTTGGACCAGAATTAAAAATGCACCAAGTTGGTATTCCTCGTGAAATGGCTGCTAAATTATTTGAACCTTGAATTATTAAAGAATTAATTAAAGGTGACAACCAAATTAACTCAATTAAAGCTGGTAAAAAAGCTGTTGAAAATTTAGATCCTATTATTTGACCTTATGTTGAAAAAGCAATTCAAGGTCGTCCAGTGCTTCTTAACCGTGCACCTACATTGCACCGTCTTTCAATTCAAGCTTATGAACCTGTTTTAATTCGTGGTAAAGCTATTAAACTTCACCCACTTTCATGTACTCCATTTAATGCTGACTTTGATGGTGACCAAATGGCAGTCCATGTTCCTATTAGTGATGAAGCAGTTAGAGAAGCCAAAGAATTGATGCTCGCTTCAAGAAATATTCTTGGGCCTAAAGATGGTGAACCTATTATCAACCCAGGTCAAGATATTATTTTAGGTCTTTATTACTTAACTCAAGAAGCTACAGATGATAAAGTAATTGGCGAAGGTCGTTATTTTGGATCATATGATGAAATGATGACAGCTTACGAAAATAAACTTGTTTCACTTCATGCTCGTGTTGTTTTACCAATTAGTGAAATCAATAAAAGAGTTATTCCAGCTGATGGTCAAGACCGTTACATAGTTTCAACTGTTGGTAAATTTATCTTCAACTCAATTTTCCCAGATAGCTTTGAATTTATCTTTGGAAAATATGTTGAAAGAAATATTAAAGAAGTGGATGGCAAAAAAGTAATCAGTGAAAAAGAAGTATTACACACTTCAGGTGATAAAAATCAACTTAAAAATTACTTATTACCTTATGGTATGAATTTCCCTGAAATTATTAAAAACATGCCACTTAACTTACCTTTAGGTAAAAAAGATATAGCTAAAATAGTACGTCGTATTTTTGACCGTTATGTAGCTATTGTTACAATGGAAGACTTAGCTGCTATTATTAGCGACTTAAATAGTGAAACAATTAACAACTTATTTGATAATTGTGCTAATTTAGTTGATTACAACAAAAACACTATTCCTGTATGACATACAGAAAACTTAGTACGTATAATAAAAGAAGAATACGAAAAAATTAATGAAGAATTAATGATTGAATATCGTAAAGTTGATCAAGAAATTGGCTGAAGAGTTAGTGATTATACAAAAATGCTTGAAAGAGTATGATTTAGATATACAAACTTTGTGGCTGATGTTTTAGACAAACTTAAAGCTTTAGGATATCATTATTCAACAATTTCAGGTACCACTATTTCAATTAGTGATGTTACTACTTTACCTTCAACAAAAGACAAAATTAAAGAAGGAGACAAATATATCGAACAATTAAGAGACTACTTTGAACAAGGCCTTTTAACTGATGATGAAAGATATGCTTTAACAATTCAAAAATGAGCCGAAATTAAAGACTCAATTGAAAAAGACTTAAAAGAAGTTACAAAAACTGATCCAGATAACCCATTATTTATGATGTTTACTTCTGGTGCTCGTGGTAACTCATCAAACTTTGTTCAATTAGCTGGTATGCGTGGACTTATGAATAACAACACTAAAATTCTTAAAGCTGACGCTGAAAATGAACGTGTTGTTCGTTCAACTGTTGAAATTCCAGTTAAATCATCATTCCTTGATGGTCTTACAGCTTATGAATTCTATTCATCAACCCACGGTGCTCGTAAAGGTCTTACCGATACAGCTCTTAACACAGCTAAATCAGGTTACTTAACTCGTCGTTTAGTTGATGTGGCTCAAGGTATCGTTCTTCGTGAAGATGATTGTGGAACTGACTATGGTTTCGAAGTTAAAGATATTCTTGATACAAAAACAAATACAATTATTGAATCACTTGGTGAAAGAATTGAAGGTCGTTTTTCAAACGTTCCTATTTATCATCCAAGAACTAAAGAAATGATTCTTGATGCAGATCAATTAATTACTCCTCAAATTGCTGAAGAAATTGTTAAAGCTGGTATTAAATCAGTTGAAATTCGTTCAGTACTTTCTTGCTATACTAAAAATGGTGTATGTAAAAAATGTTATGGTAAAGACTTAGCTCTTAACCGTGTAGTTAACATTGGTGAAGCTGTCGGAGTTGTTGCAGCTCAATCAATTGGTGAACCTGGTACACAGCTTACCATGCGTACATTCCATACTGGTGGTGTTGCTGGTGTTGAAGATATTACTGGTGGTTTTGGTCGTTTAATGGAATTAATCGATGCCTATGATTCACCATGAGGTAAACCAGCTGTTATTGCAGAACACTATGGCAAGATTACAAAAATAGAAGATAAAAAAGATACTTTAGGTAATGCTAGTGATTATCAAGCTATTACTCTTGAATATGAAGATGAAGAAGGCAATACACAAAGCAAAATTTATGCTGCTCGTAAAGATCGTCGTCTTAGAGTTAAAAAAGGTGATAAAGTTATCCCTGGACAAAAAATTGTTGAAGGACCTATTATCCTTAATGAATTACTTAAAGTTGCTGACACTCGTTCAGTTCAAAACTACTTATTGAAAGAAGTACAAAGACTTTATCGTCTTCAAGGTATCACAATTAGTGATAAATATATTGAAATCATTATTCGTCAAATGCTTTCAAAAATTGTTATTACTGATCCAGGAGATAGTAAATTCTTTAGTGGTAACTTAGTTGATATCTTTGCTTACCAACGTGAAAGTGCACGTTTAATTTCACAAGGTAAAAAGCCTCCTTTTGGTGAAGTTAAAATCAAAGGTGCAAAACAAACTCCTTTATTATCAGATTCATTCTTAGCTGCTGCTTCATACCAAGAAACTCCAAAAATTCTTGTTAATGCATCTATTGCTGCTCAATCAGATAAACTTGAAGGACTTAAAGAAAACATTATTTTAGGTCACAAAATTCCTGCTGGTACAAACTGTAATTTTGAAGAAAAAGGTAAATATGACATCCGTGATCCACGTTCATATTTCCAAAGTAAATTTGCAGAAAACTCAGATTTTCCAAATCCAAACTTTGAAGATAACTTAAATAATTTACAAAGTCAATTAGATGAATTCAACAGTGTTGATCCTGAAGAATTAGCAATAGAAGAAGAAATGGGAATCGAAGAAGTTTATGAAGATGATTACTACTCAGATTTAGATGAAAATAATGACAACAATTAATAAATAAAAAATAAATAGCTTTTACCAAAAATATTGTTTTATAGTACTATTAGTAAAATTTAGCTATTTATTTTTATTTTTTTGTAAAAAATACTCTTTTTGACTAATCAAGTTATTTTATAGTATTTAAAAAAACAAATTAAATTTGGTATATAATTAAAGTCCGGTAATATTTAAAATATTGCCGGGTGAATTTAGGCGCCCTTCACTCAGGCGCAAAAAATTATTGTTATCATTTATTTCATTGAAAAATATTTTTATCGATTTTTATAACTTTTATTAAATTACAATTAATTACATTTTCACATATCATTTATTTATATTTGGTAGTTTATTAAATCATAATGTTTAAAAAACTCTTTTTAAAGTGTTGTTAGGGAATTTTAAATTCATATCAATATGCTCCTTTCTGTGGATTTCTTTTTGTTTTAAAATAGAAATTTTGCATAATAAGAAAATCTTTAAAATTTCTTATATATATTGTTTTTTGTTAATATTATTATCGGGTATACATTTTTTATCCTCCTTTTAATATTATATTTATATTATGAATAATACGCGAAACAGGCGTGGCCTGTTTCGCTTTTATTATTTTTTTAGTATAATAAGGTATATTTATTACTTATAAATAAATTTAAGTATCTTAAATTTAATTAGGAGTCATTTATGAAAATTAAACTAAAACAAACTTTGATTATTTTATCAACCACTTTAGTTGGCACAATTGCTCTAGCAACTGGGCTTAGTTTCTTACCAACAAAAGACAAAACAATTCCTCGTGGTAAACAATCTTTCAATGATGACCATAAAAATTACAATGACCCTAATATTCCAAATTTACCTGGCAATAACGTTCCAACCAAAAACCCTAAACCTACATTAGTTTTAAAAAATCGACCTAAATCTGCAAATGCAATTACTAAAGAAAATAGCATAAAATATTTAGCTTTAGGCGACTCAATTTCAGCTGGATTTAATGCTCAACTTGATAAAGATTATCCAGGACAATTAGTTAATTCAAAAATTGAAGGTTTATCATTCCCAACTTATTTAGCTTCATTTTTCCAACAATTAGCAGACAAAAAATTAGAAAGTTTTGATAATAAAGCAGTTTCTGGAACAACCTTTAAACAATGAAATTTACTTTTAAATTATGATCCAAATAAACCTTTAGACCCAAAAGATTTGGCAGAATTAAACAAATATTTTGGAACTGATTTAGTAAATATTAAAAATGATTTAATTGCTAAAATTAAAAATGCAAACTTAATAACAGTTACTTTAGGTGCTAATGATTTCTTAAAGGATTTATTATTTAAAATGGTAAGTAACATGCCAATTTTGGATTTAATTAAGCAAATTAAAAACAAAAATTTGGACTACAATATGTTAGTTTCACATTTTGATCAAGTTTTTAGAACACTTTTTGAACAACTTGAAAATCGTCAAAAACAATTTATTCAAACTATTAAAAGTCTAAATGATAAAGTTAATATTAATTTCATTGCTTATCCTTTACCATTAACTTTTATAATGGATTTAATTGATAAAGTGATTAACAAAGGCGATACAAATAATGCTTTAAGTATTGGTCAAACTATTTTAGATTTATTAAATAAAAAGCTTAAATATACTGCAATTAGTCAAAAAACATATTACATAAATCCATATCAAAGTAGCTTTTGAAAACAAAATTTGAAGTTATTAACTCCAAATATTTTTGATATACACCCTTCAAGTTTTGGCTACAAAAAAATGGCCATGGAAGTATTTGTTAAATTAATTAATCCATCTCGTGATATTAATAAATACCATGAAAATAAAATTGAATGAGATAATAAATACTTACAAAGCGACCAAGATTCTTTTGAATACCAAATCGAAGTTAAAAATCCATTTGAAAAAATCAAAACTATTTTCAAAAATAATTATGATGGTTACTTAAAAGAAAAAGATAGTTTCTTCTTAGCTTATGAAAATCAATTAAATGAAGGCAATTATATTAATAGAGTTTTAAACAATAATGGTTTAGCTGATTTCTTATTTGTTGATTTAATTAACTCAATGTTTAAATCAAAAATTTATGAAACTATTGATCCAAATAGAAAACTAGAAAAATTCATGCATCGTGATGACTATGCAAACCAAAAATCTTTACGTGACTGATTCTTTAATGGTCAATTTATGGCTAAATTTTTATATGATGTTCAAAAACAATTATTTGAACAAGACTGAGATCAAGATAATTTACCAGGAGCTAAAGACTATCAATTTAAATACCTTTTAGAAGCAATTAAAACACAAACTTTAAAAGAAGAAAGAATTATTGAATTTATTACAAGTTTTTGTTCAAGTTCAGTAATTACAACTCAAAAAGATGAATTAAAAACCATTTTAGAAGAAATCTTTTCAAATATTGTTGCATATGAAGTTAAATATGAAGACATGCTTAAATTAGTTAAATCATTCTACAACGAAAATATCAGCAAATATATTTCACAAAATGATTTAGCAAGTTTATTGAAATTGGTTTTAAATACTAAATCAATTAAATCAGCTGTTGGTGATTTAGTTGTTGAATTAATTAACAGTGCTCCAGAATATGCAAAATGTAAAAGCATCAATGAATTATTTAATGTATTTATTAGTAATAAAAATAACCAAAACACCATTAAAAAATTCATTGAATCAATTGTTAATGACTTATTAAGTCAAAACGAATTTAAACAAATTGTTTCGCGTCTTGCAAGTAAAATAATTAAACAATATCCAGAATTATTTAAAGATATAGATGAAAATAATTTAACCAATTTAATTTATGAATCATTATCACTTGGTGGTGAAATTAATAATGCTTTAGGGCTAGTGACGAAATTTGTCAACAGCCTTTTAAATGAGTTAACTACAACCAATATTAAAGAATTTAAATTTGATAATGTAATTAAAAATACATTAACTGAAATTAAAACATTATTTGACAAAGACAATTGACAAGAATCAACATTTAATATTATTAAAATTGTTGCTAAATCTAACCTATACAAAAATCGATCAACAATTTCACAATTAATCAAAAATATCTTCAATTACTTAAATACTAAAAACTCATTTGCTAAGAGAATTGTTGATCTATTTTATGACAAAAATCAAGCAAAAATATCAGAATATATCTCAAAAGAAAATCTTACTAGTTTATTCGAAAAGGTCTTTAAAACAGAAGAATTGAGCAATTTAATCTCACAACTTGTGAGTGATTTATTAAAAACAAATCAAACACAATTGATGGATGTAACAAATTTTTTTGATGCTATAAAAGTTATCTTTAAAAACTTTGTAAATACTTCAGCTTATGACTATATTTCACCTTTAATTAAGAAGGTATTTGAGTTTCCTGAAGCTAAAGACTTATTCAAAAAAGCAATGGCAAAAATACCAGCTCAAGAAGCTAAAAATCTTAATGACCGAGATGTAGCTGATATAGTAAGTTTTGTTCTAGATAACAACGAATTTAAAGACATCTTGCAAGATTTTTTAGTAAAAGGTATTTTTGGCGATGCAATAACAATTGAAAAATTAAAAGATGCAAATAACATTATTAAATCATGACTTGCTAATAAAGGAAGTGTTGACTATATTGTTCTTAAGGCTTCAAAATTTATTCTTGATGTTTCACAACAAAGTTCAATCAAAGGAATACTAACTTCATTAATTTATGAAGCATTATCTAAAGAAAGAAATTTAGTTAAAGATATTGATCAACAAGAAATGGAAAACTTTATTGGTGACTTCTTAACAATTATTCCTTCTTTAGATGAAGAATTTAGATTTATAAAAGATGTCTTAACCAAAATATTTAATGAATTAAAAGCAACCGGTAAAGCAATTGACTTTAAAAAAGTCATGAGCGAATTTGCTAAATGTATTAAAAATAAATTATTTAACAAGAATGATTGAGAAAAGAATACAGTTAAATTAATTAAAATTGCAATTACTAAAAAAACAATGAATAAGCATCAAGCATTTTTAAGAAAATTTGTTAAAAATATTCTTGTTTATGTCAGTGAAGATATAGCATTAGGTAAAAAACTATTTAAAAGCTTATCTGAAAAAGCACACAAAATAATTGATAAACACATTGATGAAAATGACTTTAACAATATGTTTATTGACTTATTCCAACGCACTGAAGGTCTTCAAAGAATTATAGATAACATTCTTGATTCATTATTTAGCGATATTGATTCATATCAAAATGCAAATAGCTTAATTGACATTCTTAAAATTTATGTAATGCCTCAAGATCGTGCTGATCGTTTAGCTAAAAACTTAGAAGATGTTCTAAAAGAATTCTTGAAAAATGAAGCTCTTCATAAATTATTAAGAGGTCTATTTGAAGAAAATGTTGCTCCTTATGGAATTGATCCTAAGAGCCCAGGTAATAAAGCTTTAGTTGATGATTTATTAGCAAACTTGCATACATTCTTTGTTGATTTAAAAATATTGCCTAACTTAGCTAACTCAATGGTTGACAGCTTCAAAAAATACAATAACTTTAATGAAATTATTAAAAATATTGAACCGATTTTAGTCAATGGACTTAAATTAAAAGAATACTCATTTGTGGCTACAATTCTTAAAAATAATATTGTTAATAAACACAAAGAATCAATTAGAGATGATATCAACAAAGTTATCGCAGGTATCACTTCAAAAATTAATTTAGTTAACAAATTTATCGATGACTTTAATTTAATTAAACCTATTAAAGATATGGGCCTAAGTGAAACAAAAGCACGTGATTTCTTAACTCAAGTTATTCAGTCGCAAAACTTAAAAACTATAATAGATGTCTTTGTTAAAGAGCTTTTAGACAAAAACTTAGAATATGCTCAATTAAATTCATGACTTGAAGCTATAAGCAAATTCATGAGTTCACCAAGCGCATTAATAATTAAAGGAACAATTAAACAATACATAACTGAATTATTTGAAAAGAATGAAGAAGTAGCTTTTACAATTGGTAAACTTATAGCTAATGGTTGAAGAAAAGAACACTGAACTAATAGTGAAAATGATGACATTATTATTCAAAAATTCATTCGTTCATTTGGTATTGGATTACTTAAAACAAATATTTTAGATGATATTGTAGATAGCATTTTTGAAAGATTGAAGAGAATTAATACCTATGGCTTTGAAAAATTAATTGATAACTTAAGAGATGCTGCAAAAGAAGGAGCACTTAAGTTTATTAGCGAAGGTGGCAAAGTTAGATTATCAAAAATATTTGACCAAATACCTTTAATTAGAGATTTACTTGCTAAAGTACCTCAAAAAGAATTTTCAGATTTTCTTAACTTAGCATTTAGTTCAACTCCTAAAAATGATACAGAAGGATTATTCAAAGTGATGTTTAATTCTGATAAAGGAACATCTAAATTTGAAGTTGGTATTGGAGCAAGCGGAATTATCGATATTCTTAAAGGTAAAGTTGGTGATTTAGTTTCAGCTATTGCTTCACCTCTTGTTAAAGAATTCTTTAAACAAGCAAGTGAACAAAATTATAGTGATCATAATACATTAAGAAAAACTAATACAGGCTTCCAAGCTTTATGAAGATTTTATAGCTTTTTAGCTTCAGTATTGTATTCAAACACACCAAGTGGATTGTTTTGAAATGGAACAAGTTTGACAGCTGAAGCTTACTTAATGGATGGTTTTGTCAAAGCATTCAAAGATAACGTAAAAGACTATAAAACAGCCTTATTGGCAAAATATGGTGATAAAAGTTTAATAGGATTTAATAATAGTTTTGACCCTCTTGATTATTACTTAAGTGGTATGACAACAGTAAATTATTGAACTGGAACTCTTCATAGTCGCTCAAACAGTTTAAATGACTCTAGATATGGTAGTGATCATGTTCTAACTTACATTTATTACAAAGACAAAAAAGATTCTAAATTTACAAACAAAAAATTCAAGCAAGCATTAATTGAATATATGCATCAAGGTTACATGCCTTATAAATAAAAATAGAAAGAAGATATTATGAATAAAAAAGAATTTAAAAAGAGATTAATTGAATACTGCGAAACGGAAGGATTGAGCAGATATGAAGAAAATGTAGCTCATGCATTAGTGAAAAATATAAATTCAAAAAACTTTGAAGTTAACTATGACAATTTTGGATCTTTAATTTTGCATAAAAAATCAAAAGTTAAAGATGCTCCTAAATTTATGATAGCTGCTCATATGGATGAAGTTGGTTATTTAGTACGTCAAATTCATGAAGATGGACAAATTCTTTTAAGCTCAGTTGGTGGAATTTGAGCAAATGTCGCAATAGGCACTAAAGCAACTTTAATTACTAGTGACAATAAAAGATATAGCGGACTTTTCGGACACACTTCAGTTCACATTATGGAAGCAGAAGCTAGAAATAGAGCGGTAACAAATAAAGAAATTTATGCTGATTTTGGCTTTAAAAACAAAGAAGAGGCATTAAAGAAAAATGTTCAAATTGGTGATCGTGTTTTAATGTCGGGTGAAACAATCAATTTTGACAATGAAAACTTAGTTGGAGCTAAAGCTATGGACAATAGAGCTGGAGTTACAACACTAGAATATATAGCTCATCAATTAAAAGATTTAGACTTAGCAGTTGATTTATATTTAGTTGGAACAGTTCAAGAAGAAGTAGGTACTAGAGGTGCTAAAACCTCAGTTACTGTTATTGATCCTCAAATTGCAATAGCACTAGATACAACCAGCACTCATGATACTATTGGTACTATTGCGGGTACTACCGCACTAGGTAAAGGCGCAGCCATTAGAATCATGGATGGAGCTATGATGGCTAATCCAAGACTAGTTGAATTAATGTGTAAAATAGGCAGAAAAGAAAAAATCAAACACTACAAATTTGTTGCTATGGGTGGCGGTACTGATGCTAGTGCGCTTCAATATGCTAAAGGTGGAGCAGCCACTTTAACTATCTCTTTAGCTCAAAGATATCTTCATTCTCCAATTGGAGTTTGTGACATAGAAGACTTGATGGCAGCTGGTGATTTAATTGTTGAAACAGTTAAAGCAATTAATCCTAAAGTTTACGATCAAGAAATTAAATATCATTAATATTTGTGTTAAATAACTTTTACTTTATAATATTAAATAAGTTATCACAAAAATGAGGAGGTTAATATGGGTACAGGTTCATGAATCGGATTAGTTATTGGTATGGCAATCTTATTCTTTTTAATGGGTGGTTTTATTGCTTTTATCGTTTCAAAGAAAATGTTCGAAAAACAAATTAAAGAAAACCCTCCTATTACTGAAAAAATGATTAGAGCTATGTTCTTACAAATGGGAAGAAAAGCTTCAGAATCACAAATTAAAGCTGTTATGCGTTCAATGACAAATGCTAAAGATGAAAAATAATAATTGTAATAATTTGCAAGCAAAATAAAATATTTGCCCTAGTTAGTGAGCAAATATTTTATTTTTTCTTTTTAAAGAATCATCTTCTTTTTGGTCTTTTGTTTTCTTCTCCTTCAACACTACGAGCATAGTGACATTTAGGGAAGTTAGCACAACCAACAAATTTTGTATCATTTCTTTTATTATTACGAACAATTAATTCACCGCCATCATCTGGACATTTTTCATTTAAATATTCAAGTGCAATAGCAATTAATTCTAATGTTTCAGTTGCATTTTCATAAGTCTTATTAAAACGTTTTCAAAAATCATACATAATAGGTTGAACAGTAGTTTTATTTGAAGCAATTAAGTCTAATTGTTCTTCAACTTCAGCTGTATAGCCTTCATTAATAATATCTGGAAAACCATCTAATAATTTACTTAAAACTAAACGGCCAAATTCTGTATGATGTAAACTTTTATCAATATTTTCAAAATATTTTCTTTCCAAGTTTACTGCAACAGTAGTTGCAAAAGTTGAAGGACGTCCAACTTTAATATTATCTAAAGCTTCAATTAATGAACCTTCAGTGTAACGAGCTGGTGGTTTAGTTTCATGATTTGTTATTTGATATTCTAAAACATCCACTTTTTGTTGTATTTGATAATTAGGATCTTCAATATCTTCATTTTCATTTTTAACAAGATAGTAACCTGGGAATATGGTTTTACTTACATTAGCTTTAAAAGTTAACTTATTTTTTTTAAAGGTATAAGTTTTATTAGCTTTAATTGGTGGATTAATTAAAGACATAAGTGTGTGTTCATAAATTAGCTTATAAACAATGTATTCACTATTATCTAAATTAAATTTAGCTTTAGCAACTTCAGGCAGTAAGTTTATATCAGTTGGTCTAATAGCTTCATGGGCATCTTGATCGCCTGCAAAGCCTTTGATTTCTTGAAGAATATATTCTTCACCGAATTTATTTTTAATATAACTTCTAGCAATATTTAAGAAATGTTGACTCAATCTAGTACTATCAGTTCTAGGATAACTAATCAAACCACCATCACCATAACCTTCATAAAGTTTTTGAGCTGCAATTTGAGTTTGTTTAGAAGTTAGCGGACTTTTCTTATATAAAACTGCTTGTTTAAGTGGCGTATATTTAGCTAAAGTTTTTCTTGCTTCTTTAATATCTTCTACTTCTAAAACATTAATTGGTTCAATGCTAATTTGTTCTTTAACTTTTTCTAATTCTTCAGGATAAACTCAGTTTTTTTCTGCTGCTGGATGTTTATCTATATAAATAGAAGCACTTAATGTTTCATTTAATTTAGCTTCAACTTTGTGATAACCACGAGGCACAAATGCATTAATTTCATCTTCACGATCAACTACTAATTTAAGAGCAATTGATTGTACACGTCCTGCACTTGGAGTACGAGGACTATTTGAAATTTTCTTTTGCATTAATTGGCTTAATTTGAAACCAATAATTCTATCAAGCATTCTTCTTGCTTTTTGTGAATTAATTAAATTTTGATCTAGTTCTGTTGGATTTTGAATTGCTTTTTGAATAGCTTCTTTTGTTATTTCATTGTATTTAATACGGTAGTATTTTTTCTTATTTTTATTACGATCTTTTTCAATTAAAAAGTTAACTATGTGATCTCCAATTGCTTCTCCTTCTCTATCAGGGTCAGTTGCAATATAAATAACATCGGCTTTTGCTATGGCTTCTTTAAGGTTTTTAATAACATTTTTTTTACTATTGTCTAATGAGTAAAAAGGCTCTCAATTTTCTAAATCAATTCCTAAACCTTGTTCGCCTTTCCTAGTACTCATAATAGCGATATGACCAACAGAAGCAATCACATCATAATCGCTTCCTAAATATTTTTTAATTGTCTCAACTTTGTTTGGAGATTCCACTATAACTAAATTATTCATAACTTTAATTTATCACAATCTTGCAATCTTGTTATCATTTTTTTAGCTATTTTTTTGCCTTATTATATTTAATATTAAAGTATTAATATTAGAGTCAAAAATATTTAAAAATTTATTTACACAAAAATTTCACTATTGAGCATTTAACTTAATTTGCCTATTTTATAGTGCTTTTAGTCAAAACTTCAAATTTACGAATTTATATTTTTAAATATAATTTATTTAACATAATTCTTTTTATTGAAAAATAGTTATTTTTTTACTAGCTAGTAATTAAATTATATATATAATATAACAGAGTATTTTATTAGGATAATAATACTCCTTGGTCACTTAAGACCCTGAAGTCCAAAAGGAGAAAGAATGCACAAATATGAAATTATGATGATACTTGATCCTAAGGCAGATGCCAAGAAGAGCATTGACTTAGTTGAAAGTATTTTCAAAAAATCAAATGTTAAAAAAGCTGAAAAGCTTGAAAGAACTGACTTAGCATATCCAATCAAAGGATCATTAAAAGCTCAATACTTACTTTTTGAAATTGAAGCTGAAAGTCACTTAATTGCTGAATTAACTCGTCGTGTTAATATCTCAAAAGATATTTGAAGACATTTAGCAATTAACTTAGATTCTGAAAGAGGATATGGGAAAGTTAAAAAAGAAAGCCATAGAGCAAAATATGAAGAAAAGAAAGAATTCAAAAAATCAAATAAATCAGCTGAAGCTGGTGAAAAAGTAGCTGAAGAAAAAGCTGCTAAAAAACCTAGATCAGCTAAAAAAGAATCACAAGAAAAAGAAGTTAAACCACGTCAAAGTAAAGCTAAAAAAGTTGAATAGTTAATTAACTTCTCGTAAGGAGAAAGCATGAACAAAGTATTACTTGTAGGTCGTATTTCAAATGATATAAGAATGTACAGAACAAATAGTGGCATTTCATATGCTAGAACTTCTATAGCAGTAAATCGTCGTTCTTCAAATGACAAAATTACTGATTTTATTCCTATTGTTGCATGAAGAAATTCAGCAGAATTTTTAAATAACTATGCTTCAAAAGGAGCTCTTGTTTCAATTGAAGGTTCATTTACTTCATCACAATATGAAGGACAAAATGGAACAGTTTGATCATATGAAGTTACAGTTGACAATATTTCATTACTTGAACCTCGTTCAGTAAGAGAACAAAGAACAGGCAAAAGTGGAGAATATTCAGCAACAACTCCTTCATTTAATCAAGCAAATTATCAACAATCTAAAAATCAAAATACAACTAAAAAAGCAGCATCAAGCCAAACTAAAAATAATTCAAAACAAAATGAAAACCAATTTGGTGGATTTTTACTTGATGGCGAAGATGAAAATGAAGATAGCTATCATACATTTTCAATTGATGTTGATGAAAATTAGTAAGGAGAATCATGGTATTTAATAACAAAAAGAAAAATAACTTTAGACGTCGTAGATGTGAAACTTGTGATCAAAAATTAACTTATGTTGATTACAAAGATGTTGAATTCTTAAGCAAATTTATTACAGCAACAGGTCAAATTAAACCTCACTCAGCTACTGGTGCATGTGCAAAAGATCAAAGAAAAATTGCAAATGCAATCAAAAGAGCTCGTTTTATGGCACTTATTCCTTATTCAAAAGATCGTGTTCGTTTAGTTAAATAATTTAATTATTTATAAATATTAATTTAATCACCTAACTAGGTGATTTTATTTTACTTATTCACTTAAAAAATAAGTTATATAATTGAAACACTATGAATTTTGAAAAAATATTTATTTGCACAACAGACACAGTAACAGGCATTGGCGGACCTATTTGCCAAGAAACCTTAGATGCTCTTTATTATCTTAAAAAAAGACCTAAAAGTAAAAAAATAATGATTTTAGTTGCCTCATTAAAACAAGCTCAAAGTTTTAAAGAATGAACTAAAGAAGCAAATGATTTAGCCTTAAAATATTGACCTGGAGCTTATTCTATTATTACTAATAACCAAGGTTTCAGAATGCCAAATAACAAAAAATTATTAGAATTTTTAGAAAAAAATGGACCTATGTATGTAACAAGTGCTAACGTCTCAACTCAAGAACCGATTGATATTAAAGATGCTCATAAAGTTTTTCCAGAAGTTAAAAATGTTTATAACTTTGGAAAAGGCAAAGGTGTAGCTAGTCATATTATTGATGCTCAAAGTAATGAATGATTGCGATAAAAAAACTGCCTAAGGCAGTTTAAATTAAATAGTTAATTCTAAATCTTTTGAAACAGCTTCATAACTTTCTTTATCATTAAGATAAAGCAAGTTAAGAGCTGCTTTTTGTTTAGCTAAAATAACATCAGATTGAGCTTTTGTTAATTCATTTGAGCCAGTTGAAATTAAAGTTTTTTCTTTAATTGATTTGTAAAATTCTTCTGCTTCTTTTTCTGTAATTTTGTTTTTAATTAACAATGAAAAAGTAAATTCAATAATTGCGTCTAAAAGAGAATTACGAACATTTGTTTTTGCTAAATCAAAAATTTCATAACCATCTTTTTGTTTATTTTCTATTGCATTTCTTAATAGATTAGCTTTATCTGGAGGTAACATTGCTAATTTACTTTTAATAAAATCATTTACTTGTGTATCTAATAAAATAGGTGGTAAATCAAAACCATATTTATTTCCAACTGTTTCAATTGCTTTTTTCATGTAATCTAATAAATAACTTGAAGCCATTTCATTTTTCAAATTCATTGACAATTGATTATAAAGATCATCAAGAGTATTAATGCCTTCAATTTTGATTTCTTTTATGTTGTCATTTGTTAATTTAGTTTTAATTGGACGAAAAACTTTAGTAACTGTAATTTTAATGTTTTCATTTTCTGGTGTATTTACTGAAAATGATTCATTAACTTTACGATTCATTAATTGACTATTAATTGAGTTAGGATTTTCATCTTTTACAATTTTTTTATCTAAATCTTTACGTGACAATATTTCTTTTTTGTCTTTAGAAACTGAAATATTATATCTAACATAATCATTTTCTTTTGATTGGTCTTCAACTTCTTTAAGTAAAGGATAATCATCAACAAATTTTTTGTAAATTTCATCAATAGCACCTTTTGTCACCATTTCATATTTACCATCTGGTTTAATATTTATGTCAATTTTATCGAATTCAGCTTTTGGATAAATTACAATTGTAACAACAGCTTTAGCTTTATCTTTAGTAATATCTTCGACAACAACAATAGGCGAAAAAGCTAAACAATTTAGATATTTTTCTTTTAATTCTTTACCCATTGATTCAACTATTTTATTTGTATCTGATTTAAAAGCTTCTTCAATTAATTGGTCAGGTGAAATTTTCTTGCCTTCTTTAATGCTTTCCATTAAAAAATTATTTTGAATTTGTTTTCATTCTTTGTCTTTAAATTCAATGGTTTTGGTTTCTAAAACATATTTAATCATTTTTCCTCTTTTCTTTATTTATTATATTTAATAGTTCATGATTTTTTCATATTCATCTAGTGTTCCATGATAAATAAAACTTTTATCAGGAGCAATTTCAACAATAACATTAGCTACTTGGTTAACTAAAGCTCTATTATATGTTGTTACAATAACACCACCCTTATAAGCTTTTAATCCTGCAATGACACTGTCAATGCTTTCAGCATCAAGGTGATCTAAAGGTTGATCTAGAATCAAGAAGTTAGATTCTAAAAGCATCATTCTTGAAAACATCAATCTCGCTTTTTCTCCACCTGAAGTTACTTTAACATTTTTAAATACTGAATCATTTGAAAATAACATTCTTCCTAAGAAGCCTCTCATTCTTTGATCACTAACATCTTTGGTTTCTTCAGTTGAGTTGTATGGAGGTCATTTAGCAATTCATTCTAGAATTGTTATATCTTCTTTAAAATATTCTTTATTTTCATTTGGATAATAAGTGTGTTTTATTGTTTGGCCCCATTTTATAGTGCCTTTTGTTGGTTTTATCTTACCAATTAAGCATTCTAAAAATCTAGTTTTAGCAATATCATCTTCACCAATTAAAACCATTTTTTCACCACGAGCTAAAGTGAATGAAACATTTTGGAATAGTGTATTTCCTTTTTCATCAACATAAGTCAAACCTTCAACAGTTAGAATGTCTTTACCAGGTTCACGGTTAATGTCTCAATTAATATAAGGATATTTACGATTAGAAGGTTTAATTTCATCAAGAGTAATTTTTTCTAAAGATTTTTTTCTACTGGTTGCTTGTTTACTTTTAGAAGCGTTGGCGCTAAAACGAGCAATAAATGCTTTAAGTTTTTCAATTTGTACTTCTTTTTTAGCATTTGATTGTTTCATTAATTCTCTAGCTAACTCACTACTTTCTTTTCAAAAAGTGTAATTACCTGTATAAATTTTTGCTTCATTGTAATCAATGTCAACTATGTGAGTGCATATGTTATCTAAAAAGTCACTGTCGTGGCTAACTACTATAACAACATTTTGATAATCTGCTAAAAAGTTCTCTAATCATTTAATTGCACGCAAGTCAAGATTGTTAGTAGGTTCGTCCATGATTAAAATATCAGGATTACCAAATAATGCTTTGGCTAATAAAACTTTAATTTTTTGATTAGCAGTTAATTCTGACATTTTTGATTCTCATTTATTTTTTGGAATTTGTAAACCTGACAATAATTCTTGAGCATCATTTTCAGCAGTTCAACCACCTAATTCGCCGAATTTTTCTTCTAATTCTCCTGCCCTTGTATAGTCTTCCATTGTTGCTTCAGGATTTGCATAAATAGCATCTTTTTCTTGTTTGATTTTATATAAATCAGTATTTCCCATAACTACAACATCGGTTACATTCATATTGTCATAAGCATTATGATCTTGGCTTAAAACACTAAGTCTTCGGCTTTTTTCAATTATTATTTGGCCGCCGGTAGCTTCTATTTCACCAGCTAAAATTTTTAAAAATGTACTTTTGCCAGCTCCATTGGCTCCAATTATTCCATATGTATTTCCAGCTGTAAATTTTAAATTAACATTTTCAAATAATTTTTTGTCGCTAAAAATTTTACTTAGATTATTTACTTCTATCATTTTTCCTCCTAAAGCATAATAATTATTTCAATTATAAATTAAAATAAATTTTAAATTTAAAGTTAATAAAAAAAGCTACAAACTATACTGCAATAGCTGCATCTCTAGCAATTACTAAAAATAATTGAGGTGCATTTAGTTGTAAAAGTTTTAAAGCTATTTTTATATTAATTAAATCTTCTTTTACAGTGTTAACATTTTTGTTTTCATTTCTATTGTAATAGTTGTTTGATTGAAATAAAATATTTTCGCTTAGATTTAATTCTTGATTTACTTCTGTGTCATGGACTTTTATTTTTTCTAATGTGTAAACTAATTTTTTAACAATGTCTTCAACAATTTCTCTTCTAGCACTATTAATCGCATTATCTATTATTTGTTTACCATATTGATCTTTTTCTTGAATACTTTTTTCAATAGCTGCTCGATTATTTGGATTTACTTTTTCTAAATATTGATGAGTATAATCTGATATTTTGCTATTTATAAATTCATCAGGAATAATAATTGTATTAGTATTTTCTAATTCACTAATTAAGCTGTCAAAATATCTCATAACTTCTTTAATGGCATTTTGTTTTTTAGTCATTGAAAAGAAAAAGTTTTTTAAAGTAATTAGATTTGTAACATAAGGTATTTTAAGAAAATAAATATTTTGATTGGTTAGTGGCATCGAAACAGTCCTTTTGATTTCAATTACTTGCATTTCTCATTTTCTACCTAAAGGATCATTGATGCTAAAAATTTCACCATATCTATGATTAAGAATTGAATTATTAATAGTAAAGTTGTTTGTGTGGTTAGCTATTATAAGAACATTTTTATCATCTTTCATTAAGATATTATTTTGAAATAATTTAATGTTTAACTTAACTAAGTCGTTTTGTTTAATAAAATCATTTACTGAATTTTTAAATGTGTAATTTTTAATTGTATTTTCAAAAATTGCACCAATTGAATTTTCATCAAAAAAGTTGTAAATATAATTTACTGGAGTATTTTTATAGTTAATTTTTTTAAGCTCTTCAAAAGGATAATAAGCAACCACAATTTCCGCACTAATAGTGTTATTTGAAATAATATCTTTTCTAATTGCTGGACTAGCAATATAGGCTAAAGCATCACTTTTTAAAATTTCATTTGAAATTAAGTTATATATTTTTTTGCATTTATAATCAAAAGATTTTTCTAATAAAGACATTGAATCAATTTTAGTTCTGTCAATTTTTAAATCTCTAACAGTATTATTTATCATTGCCTCAATTTCATCTTTATTCATTTTAATATTTTCTCTTTTTAATAAATAATTGATCATTCTTCCTCCAAATCATTTAAAAAACCTAATATTTCATTCTTATAATATTTTGAATTATTTATTAAAATTTAATATTAAATAATATATAAACCACATAAAAAATCTCTTTTTAAAATAATTTTTTTACCAAAAAACCTGTTTTATAGTGTTTTTTGTATTTTTGAATTAAAAAAATAAAAATTAAAATATTTCTTTTTTTCTTAATTTATAAGCTTAAAAATTGCTAAAAAAATATTTAAAATTTTTATTTACAAAAAAACTTTTTAATATATAATAATAAGGCATAACTTAACAAATGCCGATTTAGCTCAGCGGTAGAGCAGCTGGCTGTTAACCAGTTGGTCACAAGTTCAATCCTTGTAATCGGCGCCATTTGGTCTGTTGGTGAAGCGGTTAACACACATGGTTTTCATCCATGCATGCACGGGTTCGATTCCCGTACAGACTACCATTTTGGAGAATTAGCTCAGTTGGGAGAGCATCGCCCTTACAAGGCGGAGGTCATGGGTTCGAGCCCCTTATTCTCCACCATGCCGTCTTAGCTCAGTTGGTAGAGCAACTGACTTGTAATCAGTAGGTCGTAGGTTCGAGTCCTATAGACGGCACCATTTATATGCTTTGCTATAGTTAGAAAAGTTTTCTTTTTTTATTTAGATGACTTTTGCAGGTGCAATTCCTTCCAAAAAGCTAGAAAAAGTTGCGCGAGTGGTGAAATTGGCAGACACGCTAGATTTAGGCTCTAGTGCTTTACGGCATGAGGGTTCAAGTCCCTCCCCGCGCACCAAATGCTTTAGCACTTAACTTTTTAATAACATCAACTTTGAATGGTTGGTGTTTTTTTATTAATTCAGCCATATCTTTTAAATAATTTCTTATATTCTCTCTTTCAAAAACTTTACGACTTCCAATTAAATAGAAATTGATATTATTGTCTGGACTAATATTTCATTTAATATAAGAATTGTTGCCACAAAAAATATCAATTGTTATATTTTTTTCAAAATAATCAACTTGCAAAATTTTCAAGTTGTGTCTAATTGCTGCATTTTCAAACATTTTAAAATTAATTATCTTATTTTTCATTTTGCTCCTTTTTATTAACTTATCTTTTTTGTTTATAAAAATGCTAAAAACTGGAAAAATTCAGGAAAAAGAGATCAAAAATACCAAATTTTTCCAGAGATAAAGTTTTTTATTTTCTATTAAAAAGGTTATTTTGAAAGGAGATTAAATGGTTTTATATCGAATCGGAGAAATTGTTTATAAAAATAATCAAAATTTAATTTTTGAAAGTCAAGGAGTAGGTTATTCAATAATCTTTCCAGATCATGCTAGAGTTGAAGCTAAACAAAAAATGAAGTTGTATCTATATGAAATAAGAAACGATTACACTTGATCTTTATATGGATTTAAAGATTTCAAAGAACGCTTATTATTTATGGACTTAATTAATTTAAATGGAATTGGACCAAGAGTTGCTTTTAACATCCTTAACCAAGGATGAGAAAAAGTTGCATCTTTAATTGCTTTAGGTAAAGTAGATCACCTAATTGAAATTCCTTATATTAATCCAAGAATTGCTCGAATTGTAGTTGGCGAATTACAAGACAAATGAGCTAAAATAACTAATACTCAAGATGCACAAAAACAAGTTAAAAATTCAAATTTATTAAGTGAAGCTAAAGAAACATTAAAAATGTTAGGCTTTAAAGCAGCTCAAATTGATTCAGCATTAACTAAAGTTGAATTAAATCAAGATGTTGAATCAATGATTGAAGAAGCAATTAAATTTATTTCTCAAAGTGGTGCTCAAAATGAACAGCTTAATGCTTAGACCAGCTAATTTTTTTGAATTTGTTGGCCAAGAAAAATTAATCTATACATTAAAGACAATGATCGAGGGTTCAAGGTATCGAAAGGAGCCTCTTGATCATATTCTTTTTTATGGTCCACCAGGGACAGGAAAAACTTCTTTAGCTAGTATTTTGGCTAATGAATTAGATACCAAAATACATTATTTACAAGGTGCTCTTTTAGAAAAGAAAAGTGATATTTTAACTGTTTTTGCTAATGTTAAAGAAAATGATATCATCTTTATAGATGAAATTCATAGCATGAATAAAAATGTTGAAGAAATTATTTATAATGCTATGGAAGATTACAAAATCGACATAATTATAGGGCCTGAGGGAAATAGTAAGGTTATGAGAATGAATCTTAAACCTTTTACTTTAATTGGCGCAACTACTAAAATTAACTTATTAACTCAGCCACTTAAAGATCGTTTTGGTTTTATCGCCAGACTTAGTCAATATAATGAAAAAGAAATAGCAAAAATATTAGAAAACAGTGAAATGGTGTTAAATATCAAAAGTGAAAGAGGAGTCATTCCTTTGATTGCAAAATATTCCAAAGGGACACCAAGAGTTGCTAATCATTTATTAAAAAGAATATTAGATTTTTCAATTCAAAATAAAGAAGATGAAATAACTAAAAAGACAACTTACTCAACATTTAAACATCTAGAATTATATGATCTTGGTTTAGGCCGAGAACACATTGAATATTTAAAAGTTTTAAACGAAACATTTGAAAATAAATTTGCTGCAATAGATTCAATTGCTGGTGTTTTAAATATTGATCGAGATATTTTAATTTATGAATTAGAACCACTCTTGCTTTATTTAAAATTAATTGCCAAAGGGCCAAGAGGACGAAAAATTACAACAAAAGGAATAGATTATTTGCTAAAAAATAAGTTAGATATCCATTATTAGTGAATTAATTTTATTTTATTAATATTAAAATTTATATTTGATATATAATTGAAAAGTTATGTGATATAAACTTAAGCCTTTCATGAGAAAAATCTTTAGTTGAAGCAACTGAAAAAGATGAGTTGTTTTAAGTATTACATTAATTTCCACAATCATTGCTATCGTTTGTGGAAGTCTTTTCTATATTTCTAAGAAGCCAAACTTGTCAATTGAATATGGTGGAGGTGTTGAAATCTTAGTTCAAGCACAAAAAAATGGCAAAAAAGTTAACGATAAATATGCCAAGAATATTGCTAACACTATGGATGACCGTTTAACTGGTGGTATTGCATTTAATGGAACTACTGTTAATGTTGAAGGTGATGGGATTTTAAAAATTACTCAAAATGGTAAATTAAGTTCAAAAGAAAAAGAAGAATTAGAAAAAATTGTTTCTTCAAAACCAAATTTAATTCTTACTGATGGCGATATGATTCCTTTATTTGTTAGAGGAAAATTTAATGAATCTAGAGAAAAAATAGATTATTCAAGAATTGAAGATTACATTCCACCTCTTAAGGAAAATGGAGCAAAATTTAAATTTATTCCACCAAGTGAAAAAGGTCGAAGTGGCTATTATGGTATAGATGTTTCTTTAAGAAATAACGCTGCCGCTGACGAATGATACAAGGCTACTGAATATATTACCAAAAATAATAAAGATCCAAAAAAAAGAAAAATATTGATGTGACTTAATCTAGATGCTTTAGTTAAATTTGCAAAAACTGAATATGCATCAGATTGAAAAGAAGCGGGAGAAAATCCATATAATTTTGTTCATATTGGTAATAAAGCGTTAAATGATAAAGGAAAACCAAACGCTTTAAAAGAATATGAGTTTAAAGGAACAAACTACTTATTAAGTGAAGCTGGTCTTAGGGAACCATTGAAAGGTACTCAATTTTTCATTTCAGGTAGATTTAATGAAGCTCAAGCAAAAAAAACAGCAGCTAACATTAATTTTGGTTCAGCAAACTACGATTTAAAAATTTTATCTTCTAACTATATTGAAGCAAGCAAAACCTCAAATTCATTTAGAAGTGCATTGTTAGCTGGAGCAGTTGTCTTTAGTATTATTGCAGTCTTTATGATTGTTAACTATGGATTACTTGGTGCTTTAAGTACAATATCAATTGCTTTATATATGTTCTTAACTTTATTATTGTTCACAGTTTTACGTGGAGAATATTCACCAGTAACAATTGCTGCTTTAATTATTGGTATTGGAATTAGTGTCGACTCAAATATTATTACCTTCGAACGGCTCAAAAATGAGGTTTATGCAGGTGATAGGCTGAAGAAGGCTATGAAGAATGCAAATCATAGGACATTAGGTTCAATTTTAGATGCAAATATCTTAACTTTATTGATTTCATTTGTTCTATTCTTCTTTGGTACTTCAACTATTAAAGGCTTCAGTATTTCACTTGTATTCTCAGTTACATTCATCTTAATTGTTATGCTTATATTTACTAGAGGTTTAAGTGTATTGCTAGTAAATACAGGCGCATTCGACAAAAAACTGTGACTTCTTGGAATTAGACAAAAGAAAATATTAGCCCCTAAAAAAGAAAATAAATTTAACAAATTTGATTTTGTTAAAAATTCAAAATGATTTGGAATTGGTTCATTAATATTTATTGTTGCAGGAATTATTGTTTTTGCAGTATTTGCTGGAATTAATAAAGATTTCTGAGCAGGATTCAACAGATCTATGGAATTTGGTGGCGGAGTTAGAATTTCTATTGAAAGTGCTAATAGTGCTTATGTTAATAGTGATATTTTGGACATTCAAAACAAATTTATGGCTCATATGAATAAATGAGGAATTGATCCGAATAAAATTACATTCCAAATAGTGCCTATGGATTTAGGCAATGGGAACTTCAAACTTCAAATTGAAACTTCTCAAGACTTAAGTAATGTTTTAAGTAAAATTGAAAGTGATGTTGCAACATTCGTTTCTTCAGGAAACAAACCTAGATTTATAGCATATGCAATTTCAACTTCGCGTGCTTTTGACTTACTTAAATACTCAATGATTGCTATAGCTGGAGCATTCCTTGTTGTTATTGTATATACCTTTATTAGATTTAAATGAACTTATTCAATTGCAGCAGTATTTGGCTTATTACATGATGTTTTAATGTCAATAGCATTTATTGTTGTAGCTAGATTGCAACTTTCACCAATTATGATTGCTGCATTATTATCAATTACAGCCTTTTCAATAAACGATACTATTATTGTTTTTGAAAGAATTAAAGAAACAATTAATACTCAATATCATGGTGTTTTCTTATCAAAAGCATTAATTAAAGAAATTGCAAACAACGCAATTAAAGATACATTCAAACGTAGTTTATACACAACATTAACCACTGTAGTATCAATTCTTGTTTTATTAATGTTTGGTAATGCTACTGATTTATCATTCAATATTGTTATGATATTTGGACTTGTTGTTGGTTCATATAGCTCAATCTTTATTTGTACATGACTTTGAATTAAACTTGAAACTAAACGTCAACTTTGTATCAAAAAACGTGCAGATAATAGCTACTGAGTTTTATCTGGATCAGAAGAACAATTATTCCAAGGTATTAATGATTTTACAGCTTAAAAAGAGACAGGCTAAAGCCTGTTTTTTATTTGCCTTCTTATAAAAAATGCTTTTATTTTTATAATTTTTTTATAAAATTATAAGGCACACCAATAAGGAGAAATATGTTTTATTTAATTAAAAAAACAAAAGGAATTTCATCATTTTCTTGTATAAAAACATTTGCTAAAAAAATGAATATTAAAAAAATAGGACACAGCGGAACACTTGATCCCTTAGCCTCTGGCTTATTGTTATGTGCTAGTGAAGATGACACAAAATTAATACCTTATATTAGCCATAAAAGTAAAACATATATCAGTAAAATAATTTTTGGTAAACATACTTCTACATATGATTCTGAAGGTGAAATTACTAACACTTCAGAAGTCAAAATTAAGGAAGAAGATTTAGAAAAAATCAACAAATGATTTTTAAATCAAAAAAGCCAAATACCTCCTATTTATAGTGCAAAAAAGATAAATGGTGTTCGTAGTTATAATTTAGCTAGAAATGGCCAAAAGGTTGAGTTAAACACTCAAAAAATAGAAGTTTTTAACTCAAAAATTTTAAATTTTGATTATCAAAAACAAGAGTTAACTATTGAGTTAGAAGTTTCTTTTGGAACATATATTCGTTCCTTAGCAAATGATGTTGGAATAGCATTCAATACTTATTCCTACATGTCAGAATTAGAAAGAATTAAAATAGGCAAACTAGCTTTAAGTAATACCAAAAATGAATATGAGCAAATTGAAATAAATAACTTATTTGATCTTGACTTTTATAATGCAACAGATAGTGAAATTGAATCTCTTAAAAAAGGTCAAGTTATTAACGCTAACAAAAAATTAAAAGACAATAAATATTTATTAATTAATAAAACAAAAGATCAAAACCTAATATTAGGAATTTTAGAATTAAAAAATAACAATTTAAAAGTTGTTAAATTATTTGGCAACCGATTAGAGGAGTAATTATGAAAAAAGAAGAATTAAAAGAAAATATTAAATTAGCTGCTTTCGATATTGATGGCACAATTTTACCTTATGCTCAACCAGAATTCAGCAATAATATTGAATTAATGTTTAAAAAACTAGAAGAATCAAACATTAAATCAGCTCTAGCCACAGCTAGGGAATTTGTTACTATTGGTAAATTAATGAACAAAGCTTCTAACCTTGATTATTTTATTGGCGCTAATGGTGCTTTTGTTTATGACTGCAAACACAAAGATTTAATTTTTGAACGCACAATTAAATTTGAAGACTTCAAAATTCTTTATGAATTCTTTAACAAATATATTACCTGTGAAGGATTCACGATAATGGATCGTAATTATGGTTGATATTCACCAAAAATGAATATTAATACATGATTCTTATCACCTCATCAAGCCAAAATGAAACCAGTTGATTATGATGCTGTGGAAAAAGACCACCTTCATGTCATAACTATTACAGCTAAAGACATTAAAAGCACAAATGATTGTGCTAAAAAAGCAAAAGAATTAATTGAAAAATACAACATGGATTTAGAAGTTAATTCACAATGAGCAACTGGTGTTTTTGTTAGTCCTAAAGGAATAACCAAATCACACACTTTAGATTGATTAGCTAAATATTTAAAACTAGATTCTAAAAAGAATTTAATTGCTTTTGGCGACAGTTCAAATGACTATGAAATGATTCGAGATGCAGCTTATGGTGTTACTATGGAAAAAGCAAGTGAATACTTAAAAGCTGTGGCTAATGATGTAGCTCTTGATCCAACATCTGATGGAGCTTACTTTAAACTAAAAGAATTAGACTTAATTAAATAATTTAATTTATAATTGAAATATGTCTTTAAAAGTTTATACATTTGATAATATTCCTAAATTTCAAAAACCTATTTTCATAATAGGCTCTTTTGAATCATTCCATTTAGGTCATTATAAACTTTTAGAAAAAGCTCGTGAATTAGCAAAAGAAAGTGAAAAAGAAAGAGACATTGTTTTAGTTTATTTTGAAGATATTGAGAATTTATCTAAAACAAAAAACTTAATTTTTTCAGATGCAAAAAACCGCGTTCAAGAGTTTGCAAATTTAAAAATAAATTTTGCTATAAGCCTGTTTTATAGTCAAATTAGTCATTTAAGTGCCCAAGATTTTATTGATAAATTAATAGCAAATCAAGATGATTATTCTTTTGTAATTGGTGAAGAATTTAAATTTGGCTTTAAACGTTCAGGCAATGTTCAATTCTTAGAAAATCATTACAATAAAGATAAAGTTTTTGCTCAAGAAGTTGTGAGATTAAAAAACAAACAAAAGATTTCAACTTCATATTTAAAAGAATTAATTGAATTTGGAGACATTGATTTACTTAACACTATCAACATGTTTCCTTATAGTTTTAGTGCTAAATTTTCAACTATTGAAAATAAAATTGAATTAAAAAAACATGAACTTTTAATTCCGCTAAGACAAGGTATTTATGCAGTTTTAGTTGAATTAAATAATTACAATTATTATGCATTAATGCAAGTTAATTTTGACACTAAATATTTCATTGAATTCTTAGATTTTAAAATCAAAGAAAACCAAAACATTGATGCTCGAATTAAAGTTTTAAAAGCGATTAGATTCTTTAGAAATAAAGAACTAGAATCAATTAATGAACAAAACAAATTAAATGCCAAAAGCATACTTTTAGATCTTAATAATAATTAGTAAAAAAACAAATTACTTTTATTTTGCATTACTTTTTGCATCTAAAATAAATATTTGGTTTTTTATTTTTATTTATTATAATAATAAATATTTATTTAAGAGGAGAACTATGGCTGTTGGAATTATTGCTGAATATAATCCATTTCACAATGGACATATTAAACAAATAAACTGAGTTAAAGAACATTTTCCAGGTGAAAAAATTGTTGTTGTTATGAGTGATAAATTTAGCCAACGTGGAGAATTAACAGTTGTTCCTTTTAGTATTAGAAAAAAATATGCAAAAAAATATGGTGTCAATAAAGTAATTAAATTGAAATTTGAAGAAACTGTGCAAGCAGCTCATATTTTTGCATACAATGCTGTAATGAAACTTTACAAAGCTAAAGTTGACAAAATTGTTTTTGGTAGTGAAAGTAACAATCCAGATTCAATGCTTTATTGCGCTAAAGTTATGAAAGAAAAACATAATGAATTTTCTTTTGCTTTATTGCAAAAAATGAAAAAATCAGGTTTAAGTTATCCTAAAGCAGTTAGTGAAGTTATGAAAGAATTAGTTGGCGAAAGTTTTGAAATGCCAAATGATATCTTAGGTTTTGAATACATAAAAGTTATAGTATATAATAATTTGCCAATTAAGATTTATACATTAAGAAGAGAAGTTGGTTATCATTCGGATAAAGTTGTAGATGAATTTGCCTCAGCTTCATATTTAAGAAAACTTATTTATCAAGGTCAAGATATTAGAGCTTATAGTCCAATGACTTTTAAAAAAGTACCAAGAACTACAGCTTCACTATATTGAAAATTTCAAAAAATAATGAATCATACTTCACTTAAGAAAATCAAAAGAATACCTTTAATTTCAGAAGGTATTGAAAACTTACTTAAGAAAAATATTACTTATCCAACTTATGAATCATTTGTGAAGCATGTACTTCAAAAAGATATACAGCTCAAGAATCCAAAGAATAATAGCTTGAATTTTAAATAGAAAGTGAAAGAAAACTTCAAATCGAAAAGTTAAAAAAATTATTAAAATTCATAAAAAAGAAGAAACACAAAATATTGAATCAAAGAGTAGATAAAATTAATTTTTCTTTTTTATTGCATTTATTTTTTTAATTAGGAAATTCTAATAAAATTAAAGTATATTCAAGTAGAAGGAGTTTTATGGACTCAACAAATAAGAAAAAAATCCTTGAAGTCCAAGGATTAAAAAAATATTTTTTTAACAACAACAAAATCAATAAAGCAGTTGATAATGTTAGCTTCAATTTATATGAAGGCCAAATTTTAGGTTTAATTGGTGAAAGTGGAAGTGGTAAAACCACTGTTGGAAGATCAATAGCAAAACTATATGATGATTTTCAAGGCCTTTTATTAGTTGATGGTAAATTAATTAACAATAAAAAAATGAGTGAAAGTGATCGTAAATTTTTAAGAAAAAATATTCAAATGATTTTTCAAAATCCTAAAAACTCATTGGATGAACAAAAAAGTATTTATTCAATTTTAAAAGAATCGTTGATAGCTAATAATATTTTAAAAATCAAAACAAAAGAAATCTTTAAAAATTGAAAAAACATCAAAGAAATATTTGATGCAGATTTTTTATTAAAATATCGTACTTTGCAAAAAGAAGGGCTTAACTCTATAAACTGTTTAGCTCAAGATTTTATTAATGAATGAGAAACAAATCAAAAAAGATTTGAAACAAGTGATTCGCTATTACCTGTTGATTTATTTAAAAATATTTATTCATATTTTGAAGAAAAACAAGATTTACAAATTCAAATAATTGATAACTTACATAAAGATATTAATCAATTGTTAGAATTCTATGAACAAAAACAAATTGACTATACTAATGGTAATTTTCCTTTTGGCATATTTGACTTTATTGAATTAGAAAACGAAAGAAAAAAAGCTTTAATTTTAACTAAAATGTCAAAAGAAAATTATGAATCAATGCAATTAATTGATAACTTAAATAATGAAATGAAAAACATTAAAAAAGAGTTTTGAGATCATCAAAAAGAAAATGAAATTTTACTTAAAAATTATCAAGAAGAATTCAAATCTGAAATCAAAAATTATGAATTTGAAATGAATAATACTTTAGATGAAGAATTATACAAATTTGACTATAAAAAAGCTTTTTTAGCAAAAATGCAAATTGATTTATTAAATAAAAATAAGAAGCTAATTAACAACTTATTAGTTAAAGATATTAAAGAACTTATTTATGATTTAAAAAACTATAATTTAGATTTTTTTAATAAGTATTTCCAACCACTTGAATTTAACAAAAAAATCAAAGAAAATATGCAATCAACTTATGAAACATATTACGAATTTGATTTCAATAAATACATAATCAGAAGTAATTTGAATTTAACTCAATATGAAGAAAAAATTGACCACATTAAAAAATTAATTTCTAAGCAAATGATTCTATCTAAAAATGCCGGTGATGTTAAAAATTCAATTGAATATTTAAATGAGTTGAAAACAAAAATAGAAAAAATTGATCTTGAATTTGAAGATAAACTTGTAAATCATAAAGAAGAAACACTTAAAAAAGTGAAACAAGTTGAAACTGAATTAGATGAAAAAGAAAAAATTAATAATGTTTTAAAAATCAGGCAATCTAATTTTTATCAAAATTATTCAGACATCAAATTACTTAAACTTTTCAATACTTTTTTAGCTAATTGACTTGCAAAAAGTAATAAGAATGAAAAAGTTGAAAATACAAATTATGCAAATAATTTACAAAATAAATTAAAAGATAAAATTTTAACCTTAAAAGCATTAAATATAGAAATTAGCCAATTACAAGAAGACTTAAAAACTGTTAAAGAATTTATGGGATTAAGTCATAAAAACTCAAAAAGTTTTTTTGGAGTTTTCAAAGACTTTTTTACTAATCAAAAAATAAAAAGAAATGTTAAAGAATTATTATGCAAAAACATAATTTATAAAGCTTTAGAAGATGTCGGATTACTTAAACAATTTGCTTACCGTTATCCACATGAATTCTCAGGTGGTCAATTACAAAGAGTTGCAATAGCTAGAGCCTTAATTGTTGAACCTAAAATAATTATTGCTGATGAACCTATTGCTTCACTAGACATTTCAATTCAAGCACAAGTAGTTAACTTGCTTAAAGAACTATGCGAAAAAAGAAACATTGGAGTTATCTTTATAGCTCATGATCTTTCGATGATTGAATATTTAGCTGATGAAGTGCAAATTATGCATCATGGTAAATTTGTTGAAGGCGGTAAAACCAATATTATCTACAAAAAACCAATGCACCCTTATACTCAAAACTTATTTGCCTCAATTCCTAGAGTTGAAAATGCTAATAAAAAATTTATCAACCAAGACTTTGAAGCTAAATATCTCGAAGAACAAAAATTTTCTAATACAACTGATTTATACCTAATTGAGCCAAATCACTATTTATATGGAACTAGAAATCAAGTGCTCAAGTGAATTGAAAAATATAATTTAGTTAATCCGGTTTTAGCTCAAGAATTAGAATATCAAAAGATAGCTAAAAACACAAAAGATTCACCTTTTGATGGACTTAATGAAATACCTGAAGGTGTTGATTACACTCAAGTTATGGATATTAGTATGTTCACTTCTGAAATTAAAGTTTCAAAAACAACTTTCGATTTAAACAAAAAAAGTAAGGAAAAATAATGCTTAAGCTTTTTTTTACAAGTCAGCATTATTTTCTTTCAAATTTTTTTAATTTTGCTCGTTAGCAAATTCTTTGTATTTACTATCAACATCTTGAACTTTGTTATGAATATCTTCATACCATTTTTCAAATTCTTTACGATATATTGATGAAATAAAAATATGACCATTGCATTTTTTTGATTAATAATATCAATTTTGTTTATATTTTCTAAAAATTAATAATTAGCTCATTTTTAAAAAGTATGAAAATTAATTCTAAATTTTTTGAATTCATTTTTTTCTAGATCGCTTTTGCTATTAATAGTTTTTAGAAATGTTTTAAGATAACTTTTTTCCTTGTTATATTCTTCTTTAACAACTAAAATTGGCTTACAACTAGCAGCCAAAACTGGAGTTAATAAAGCAAGTGAACTAATTGTATCTAAAATCAATTTTCTTATTCTCTTCATACTTCTATCATAGCAAATATAATAATAAAGGGAAATTTGATATTATCAAATGCAAAAAATACTTGTTATAATTAATAAATCACAATAAGGAGAATTATGCCAATTTGAGGTCAAATATTATTAGTACTTTTTTCATCAATATTTTTATCAACAATTTTTGTTGTTTTAACATATAAATTCTTTTTGCCATTAAGCACTATAATTTCCACATTTGCTAAGAATTTTTTAAATAAAAAAGTTAATATAAAGCAAGACATTTTAGAATCTTTTTATTTTAAAAACAACAAAAATAGAAAAATCTTTGTTTCATTAACAATCGTTTCAATGTTAATTACCTTTTTATTGGTTTTTTTACCAATTTTTGTTAGTTTAAATAATTATATTTATTTGCCTTTAATAATTGTTTTTTTAAGTTGTTCAACTATTTTTGCTTTTGTTTATTACGCTTTTTCTTATGCTACAGTTCGATTACAAGAATGAATTGAATCAAATAAAGGATATGAAGAAGAATTTACTTTTTATAATAAAGCATATGAATATCAAATCGATTTTTTTGATTTTGATTTAAATAGTGCAAAATATATGGTTGTCGAATCAAATAATTTATTGCCGGCTTTAAAAGTTAAACATTTTATTAGCTTTAAAAAGTTACAAAAAATATACAAAAAAACACTTCATATTCAACTTTTAGAAAATGAATGTTACCTTTATTCATTGATTAATACTGATCATTGTTTTATTAAAAAAACCAAGGTCAAAAATATACAATATTTTGCCCTATTGCAAGATTTTATAGAGAAATTTAAAAAAGTAAGAGCTTTCTTAAATTTAAATTAGCCCTTACTTTTTTAAATAAAAATCTCACAATTCTTGTGTCATTTTGTCATCACAATTTTCATCATTACCATTTTTTAAAATATATTCATATCAATTTTTATGCAATTGTTGAAAACTTTGAAGTAATCTTGTTGCATATTGATATATTTTTCTATTTCTTAGTGATACTGGACCAGGTTTTAAAGTATCTACTTGAACAATATATTTAGAAAAATTCACTAAACTTTCTCTTAATTCTTCATATTTTTCTTCTTGAATATCTTTTTTATTTTCAAATTCTTTGACACATTCTTTTGATTTCACTATCTCATTATTGAATTTATTTTCAATAGCTTTTAAATTGCAGCCACTTGCTGAAATAGTCATTGAAATTGGAATAGCAAAAGAAGAAACAATTAAAATATTTTTGATTTTTTTTCTTTGCATTTCTAACCTTTTTTTGTTACGAAATTAAGTGTTTTTATTATTTATTAAGATAATATTATAACAAATTTCAACATAATGAAAAGAGTTGTGAAAAACTTATGAGAATTTTTCAAAAATTATCTTAAACATTTAAATTTGTATATTAATAAAATATAATTTTCTCGTGATAAATAATATAGCTCAATATACCATAGTTATCCATAACTATACATTCTAAAATTTATTTTACTTATATTTATTTTTGTTTTATTTGTTATTTAGCAATATTTTTTATCATTAAAATACATTTTATTTGAAAGGAAGTCAAAATGAAAAAAGCTAATAAGCTTATTATTTCTCTTGGTACAGCATTTGCAGCATTGTCTCCAGTTGTATTAAGTGTTGGATGTGGAGGTGGAGGTTCTTCCAACCTAGATTCAATTAATGGTGAAAGTACTCCATCAAATGAAAACTCAGCTTATGCAAACGATAAAAAAGGTTACAATATTAAAGCCAATGACAAAGTTCAAATTGCTACAACTTTCTCTAAAGGTAGAGCTCAATATGAAGCTTTAAATGATATAATCAAAAATTACAACAGATTAATGAAAGATCAACCTGGTTTTAAAGAAGTTGAACAAGTTAGTTTAGGTTCAGGTTATGACGGTCAAGCTGACGTGGTTAGAAAAAAAATAACAGCCAAAGAAGCTCAAATGCCTAACTTACTTCTTAACTATTCATCTTTAGCATCATCTCTTGCCGAAAGTGGAGTATTGTTGAACTTTACAACTAAAGAAAATGGGGGCAAAGAATCAATTTCTAATAGTGATTTTGCAGAATCATTTTCAAGAGCAAATAGAACAACATCAAAAATTAATAATGGTGGTTCATGATTAATTCCTATTGCTAAAAGCTCAATTAACTTAGGCGCTAATGGTCCTGTTCTTTCATACGTAATTGAAAGTATGCTTGCAAAAGGGGCTAAAATTGATCCAAGTTGAAAAGATAAATGAGATAAAATCAAAAAATCAGGTTTAAAAGATAGAGCAGCCGTTATTGAAAACTGAGGTGCTGTTAAATCAGATGTTAGTGCATTAAAATTAAATGAGTTAACAATTAAAGAATCAACATTCTCAAATATTTACTCATTATTAGAATTTTGTGACAAAGCACAAAAAATGTTTGAAAAATCATCAGCAGTGACTACTTTAGAAGGACCACATGCCTTAGGTATTGATGATATTACAGGATTTATTCAAACTTTAGGATATGCTTCAGTTGGAGCCGATCCTGAATCATGAATTACAACAGTTGTTGAAGAAGATGGCGAAAGAAAAATAAGTTACAAATCATTTGTGGAAGCTACATCACAAGCTAATAGTAAATTTAAAGAACTTTATGATATGTTTGTTCCTAAATTGAAATCTCAATCTGTTGTTTTCCAACAAAGTGGTGCTTATACATCTAGTGAACAAATAAGACATAACTTTATGTTTGATATTGGTTCAACAGCTGGATATACTCATAACTTTGCTAAAAAAAGCGAAACATCTATTGAAATTAAAAATAGTGAAGGTAAATTCTCTTCTGGATGAAATGAAAACAATAATAAATTTGCCCAAATTGTAAAACATGATGATGGAACATATCGTATTGGTGGTCACTTAAATAAAATTAAAAAAGACACTGAAAAACTTGAAAATTATGAATGAAAAGCAAAAACCGCAGATATTTACAACAAATTAGCAGGTTATAGTCAAATTCCTACTGGAACTAAAAAAATTAGTTCACCAAGTGGAACAAATGTTTATGCTTTCATGATTGAAACCAAAAATAAAGATGATATTAAAACTTTAGATGCAGCTTTAGCAGATGGAAAACTTGTAAAAGTTGGTGAATTTGTTAATAATCAAAATAAAGAAGCTATTTTATACCTTGCTTTTGGTAAAGGTGCTGGTATTTCAGGACTTAAAATTGTTGAAAAAGGTTTAGATTCTCTTCTTGAAAAAGACGAATTCTTTAAATTTGCAACTCCTTCAAAAACAAAAGACACTGATGCTAAAAATGTTTATTATGCACAAGGTCCTTCATTATTTGGAATTAGATCTAGTGATAATGCAATGAATCAAGCAACAAGAATGTTTGTTAAATGATTTGTAACATCAGGCGCAACTGAATTAGAATATAAAGATCAAGAAACTGGAAATGTAATTAAAAAAGTAATAAAACCTTCTTTAATGTTTTCAGAACAAGCATCATATATTTTTGCTGTTAAAGATTTTGAAAAAACACCAATGACAACAACCAATCCTTACTTACGTGAATGTTTTGAAATGTTTAAAAAAACAATTGAAGATCCAGAACACAATGCTATCTATGAGGAATATGGTGATCCAAATGGTGATGCATTTAGAGGTGCATTAGGAACAGCATGAAGAAATGTTTATGGTAACAAAAGCCCAGGACAATATAAAGATCAAATAATTGACGCTGTAGTTGCTTCAAACAACAAATTATTTAATTAATTTTAATTAAAAAAACACAATAAATTAGATAGAAATATCTAGTTTATTTGTATAAACTTAAAAATTTATTTTTAAAAAAGGACTTATTATGAAGTTTAAAAATAATACTTTAAATTTTTGTTTGAATACAATTGTTCCTTCAATGGCTATTGTAGTTTGTTCTTCATGTCAAAGTAAAGATGACAAAATTTATAATGAAGCTTTTAAAGAAGACATCAAGTTCGATTATGTAGCTTATAATCCAGCTACTGGAAAATATGATATTGTTGACAAAAATATTGAAAATCAATTGACAACTAGTTTGAATAATTTATCAAATATTTCAAGAGAATTTACTTATGATGGTGAAGAATATAAACTTGAAATAGATACAATTTCTCTTGCAAGAACATTTGATAAAAACAAAACTTTTAGTGAAAACTTGAAAGATCCAAACTTTATTAATAATCAAAGGGAATATACTTACAAAATTTTCAAAAAAAATCCTAAAGGTAAATGAAAAGAATATGATAAGTATGACATTTCAAAAATGACAAAGAAATTTGACGTTAAGTTAAATTTAGTTGACAATTTTGACAAAATGGATACTAGAATTGATTATTCAAAATTACAAGCTACAGCTTATGATTGAGATCAAATGGCAAAAGAAGGTTTAAATTACTATGATGGCGAAATCGTAAGTTGAAATGATGGAGATACTCCTAAAGTCAAATATTATGATCCAAAGAAAAAAGAGATGGTAGTTGAAACCATTAGAATTCAAGGAGTTGATACTCCTGAAAAAGCTGTTGGCAAAGAAATTGCTCCAATTTTTGAAAAGAGATTCGCGGAAAAATCATCTGAATTTGCTAATGTAAATTTACCAGCTGGTACAAAAGTTAGATTTATTTATTCTAATAAAGATGCTTTTAAAAGAATCGTAGCTCATTTATTTTGAGGCGAAAAAAATGGACACAAGTATGTGTATGAATATTCTGCTGCTATTACTAGATTTGGATTTACATTGCCTTATGCTGATGGTACAGAATTAATAAATATGACTAATGCTGGTAAAACTCAACATTATACATTCTTACAAATAGGTGAAGCTTTTGAAGCTGCTATGGCTGAAAAGACAGGATTCTTTAAATGTTTAAGTGATCCTGATGACATATCAAGATATGTTTATTTATCAAAAAGAAATGGTAAATACACCATTTTTCAAAAGAAATCAAATTCAAATGTATTTAAATTTAAAGATAAAGCTTACAAAAACCCTATAAACTAGGGCTTTAAGATTAAATTAATATTATTAAATTAAAGGAAAAAATATGATATTTATTAATAGATTAATTAATCTTTTTAAGAAAAAAACAACCAAGTTTAATAAAGAAGATGAAACAGAATTTTTACGTCTAAAGCAAGAAATAGAATCAAAGAAAAATATTGATCGTAACTCTGCTATAGAATTAAAAAATGTTTTTATCGACTTTGGTGAAACTTTAGCTGTAGATGATGTTAGTTTTAAAATTCCAGAAGGTTCATTAGTAACTCTTTTAGGACCTTCAGGATCAGGTAAAACAACAACTCTTAATGCTATCTCAGGGTTATTAACAATAACAAGTGGGAAGGTGTTTTTTAGAGGAAGAGACGTTACAAGATTAACACCTCAACAAAGGAAATTGGGTTTCGTTTTTCAAAACTATGCCTTATATCCTCATATGAGTGTTTATGACAACATCGCATTCCCTCTTAAAAATGATGCAGATTGACAAAACAAAGTTTTAGATAGAAAAACAGAAGCTATCATTAATATTAGAAATATTTATCTTAAATCATTAGGTGCTAGTGATTTAGAAATTAAGCAAATTAATGAAGCTTGAAAAATTTACAAATCAATTTATAGAGAAACACAATCAGAATTAGCTAATTATCGTGTTGAATTACGTGAAAAATTTGATAAAGCGTTTACTGATTATAAAATGTCAAAAGTTCACTATACAGCTGATTTAAGCTTAGCTTCTAAATTGGCTTTAAAAAGCTTTGAAAGCTTGAAAAAGTCAAAACATGATCAACTTAATGCTATTAACGAAGAATACAATCTTCTTAAAAATAACAATAGACTAAAAGCTGAAGAAGAATATAAACAATCAAAATTCTTAACTGATTTTGATAAAAACTTATTAAAAAGAAGCAAACCAAAAAGTATAGAAGAAGTACAAGAAGAACTTGACAAAACTCAAAAACTTATTGAACAAGTTGTTTTAGAAAACCGTAGTGAATTTTCTCATGAAACTCAAAGACAACTTGTTAAAACTGAAAATCAATTAATGAAAAATAATATTTATTACAAATATTACATGAACCTTAAAAAGGCTATTGTTAAATATGAACCATTAATTGATAAATACAAAAAAACTTACAAAGAAGTTAAAAAAGAATATAAAAATTCTGTCCGTAATAATTCAAAATTAAAAAGATTAGTACGCAATGAAAAAATTATGAAAATGTACGCATTAGTTAATTTTGAAAAAATTCAAAATGAAATTTATCAAAAATACAACTTAAAGCAAATTATTGATGAAGATCACAAATTAAAAAATGCGAACTTAACAGATGAAGACAGAAACCAAATTGTTGAATATTCTAAAGATATAATTAGCCTTAAAAAAGCAATTCATAGAGAAGTGCTTGAAGTTGCTGAACGTGTTGAAATTCTTCCAATCTTACAAAAGAAACCAACTCGTCTTTCAGGTGGTCAACAACAACGTGTGGCAATTGCCCGTGCTATTGTTAAAAAACCTCAAATTCTTCTTATGGACGAACCTCTTTCAAACTTGGATGCTAAATTACGTATTTCAACTCGACAATGAATTAGAGATATTCAACAAAAATTAGGTATTACTACAGTATTCGTTACACACGACCAAGAAGAAGCTATGTCAATTAGTGACATTGTTATCTGTATGTCAATGTCTAAAGTTCAACAAATTGGTTCACCAATGGAACTTTACAACAAACCACGTAACAAATTTGTAGCTAGATTTTTAGGAATGCCAGAAATGGGCATGTTCGACTCTAAATATAAAGATGGAAAACTTACTGTTTTAGGTGTTGAAATTCCTGGAATCAAATTAAACGATGTTGACTTCAAAGATCTTAGTGTTGGTGTTAGAGCCGAAGACTTTGAAATTAAAAATAGAAAGCAAGATGCACAATTCAGTGGCGTAGTTAAAGCTGCTGAAAACTTTGGTAAAGAAAGCAAATTAATAGTAACTTTAGAAAATGGTGAAAATATCAACTTTTTAGTTGACAATGACTATAATTACAAAGCTAAAGATGAAATTTATTTCAACATACCAAAACATCGTCTTCACATTTTTGACAAGTTAACAGAAGAAAGAATTGAATATGAAATCAAAAAATAACTTTTGAGCAAAATTAGCTATCAAGTTTTCTTGATTAACTACTTTTAGTGCTCAAAAGCAAGCTAATAGAAAAGAAGAAGCTCTAGCTGAATCAGTAGTTGAAAAGCATACTCCTGCTTACAAACCATTGATTTTGTTAGTGCCAACTTTACTTGTTATTACAGCATTTACATTAGTTCCTTTTATAATAAACATTATTCAATCATTTACTGTACAAAATGCTAAAAATGGCGTTTTACATAATTTTGAATCTCTATTTAAGGAACCAAAATTTGCTGTAGGGGTTAGAAACTCAATTTTATATGCTATTGTTGTTCTACCTTTTGTTATGATGGTATCTTTATTAATATCTTCATGTATCGCAAACGTTTATCGTAAGTGAGCAAGAGGTTTTTGACAAACAATATTCTTCTTACCTTATATAACAAATATTGTTGCTGTGTCTTTATCATTTATTCAATTTTTTGAAAATAATGGTTTATTCAACACAATTTTCAATTTAGGAGAAATTCCTTGATTAAATGATAAATCACCAAAAGCAATTATTTCTTTCTATCCATTCCTTGCAATGGTTGTTCAAGGTATTTGATCAGGGCTAGCATTTAATGTTTTAATTTTTACAACAGCAATGCTTTCAGTTGACAAAAACCTCTATAAATCAGCCTCAATCGACGGAATTGGACCAGTTAAACAATTCTTTACAATTACCTTGCCTTCTATTAAATCAACTACTACTTTCTTAGTAACAATGGGAATTATAAACGGAATAAAAGTCTTTCCTCTTGCATTATTCAATAATAGACCTGAAGAAGCAGTTTTAAAAGGTGCTTCAACCTTAATGATCTTCGTTTACTACTACACAAGTCTTGGTGGAGCAGAAAACTTATCATTAGCTGCTGCAGGTTCTATATTGTTATTTATTATTGGTATAGTTTACTCAACTATTATTAGAGGGGGATTTGGAACAATAATGAGAGTATCATTAAACTTAGGAGAAAGCAATGTTTGAAACAAAATTAAAGATTCAGAAGTGATGATCAAATATCAAAATAAGAAAAAACAAAGACAAAACAGCGCAACAAGTTAGAGAAGTCTCTGCTTTTGGAATGTTACTTGGTTTATTTCTTAAATTATTGGCATTAACATTTTTTGCTCTTATTATTTTGTTCCCATTCTTCTTTATGTTGATGATTTCTTTGATGCCTGAAACTCAAGCAAAAACATTACAATCATCATTCTCAATAATTCCTAAATCATTAACTTGAACAAACTATTCAACCGCTGCTAATACAAAAGCTAATGGTAATAGTGATTATTGATCAGCATTTGCATTAACTTTTGCCAATGTTATTTTCTCGATTATAGTTAAAATATTTATTACATTATTTGCAGGTTATGCCTTCTCGCTTAAAAAGTGAAGAGGTAAAAACATATTATGAGCTATTTTAATGGGATTATTAATTTTACCTGAAGTTGCTCTTCTTTCAGGACAATATAAAGTTGTTACTGAATGAAACAATGCAATGGCACTTAAAAATAATTTCATAGGTGCTATGGTTATTATTGCTGTACCATTTGTGGCTTCAATCTTCAATGCTTTAATGTTTAAAAATGCATTTGAATCGATTCCTTCAAGAATTAAAGAAGTTGCTTTAGTCGATGGAGCTGCGGGTGCTAAATATCTGTTTAAAATAGCTATACCTATGGTTACACCAACAACTTTAACAGTGGTAATTCTAACAGCCTTAGCTGCTTGAAACTCATACTTGTGACCATCTCTTGCTCTTGGAAGCGACTACTTCATTATTTCTACATGATTATTCCATGTTGGTCGTGATGAAAGTAGTGGTGTTCCTATTATTCTTCAAAGTATTAAAATGGCTGGTGCCGTACTTGTTATTATACCAATGTTTATTTTCTACTTTGCCTTTAGAAAAAGAATTATGAATGCTATATCTCGTCAAGGAAGTACTATTAAAGGTTAGGAAGTGAAATTATGAAAAAAGCAAATATAAAAACCAAAACAATAATTTGATTAATACTCGCTATTATTGCTTTTATACTTTTAATCATATTTTCAGTTGTATTGCATAATACTATTCAAGTTTTAAAATTATCTGAATTTATTAGTTTCGACAAAGACTATCTACATGAAGCTATGTCACAATATTCATTTGCAATTGCAATAATTACATTTAGCTCAATCATAATTCTAATAGGTACTTATATTTCATATGCAGGATTCAAAAGTTGAAAATATAATGCAACTATTTAAGGAGAATAATGAATAAAAAAATGAAATTTAAAAAATTAATGTTTATTGCTGCTTCAACTTTAGCCTTAGGAGTTGCACCTGCAATTACAATGTCATGTAATTTCTATATTAAATCTAATCCATTCTTAGAATATCTTAAAGACAAATCAGAAAATTTAAGTTTTTATAAGAAAACTCCATATTGATTACAAAAAAATGCTAAAGATAAAACAAGTAATCAATTAGAATTGGCTTACAACTTTTTTGCTCCACATCATGCTAAAGTTTTACGTTACGATTCAAATGGTGCTTTCCCTTATTTAACTTCAACAAGGGAATCAACTTTATATGATTCAAACTTTAGTGAAAAAGGCATTTTTGATAACCTTTCTTATACAGAAAGTCAACAAGTTAAAAAACTTGAAGAATTTAAAAATACAAAAGAATACATTAAACAACATCCAAATGAAGTTGGCAAATACTTAACAAAATATTTTTATCAATTAACTAATAAACAAGCTGAAGATGTTTTTATGTATTACTTCAACAATACTCAATATGGTTACTACGTTTTAACGAATTATTATCGTATGTTCAAAGTTGAAAATGATTTTATTTACGATGGAAGTGAAAAACCTCTTAAAAACATTTCAACAATTTCAGACGGTACTAATAAACGTTATTTTGACATTGTTAATCTATATGCATTAGAACATTTAGGTGATTTTATTAAAGAAAATAAAAATTTAATTAATGAAAATTTTGAAGACTTTTTTATTAAATTCTTAAATGAAAGTAAAAAATATGGTCTAAGAATATCTTCAAAAAACGATAGTTTAGTAGCACAAGAAGGTATGCAAAATGCTTTTGCTCCTGATTACTATTTAAGAAATCAAGATCGTAAACAAGAAGACTTAAAGGATGCCGCATTATTTTACAAAAATGCTGATTTAAACCCTGAAGGACAAATTTATAAAGACTTTATGAAAAATCCTGTTGAGTTTATTAAAAAACATCCAAACGTTATTAAAGCTGATGCTTCAGAAACAAAAATTAATGACCGTACAAATAAAAATTACACAATTACTGTTCTTAATAAAATAAAAAGCGAAATTAATAAAATAAAAAATTATGGTGTTAATGAAGAAACCATTTTAAGAGCACTATTTACAATTTTAGAATTTCATGGATTAAAAGATTATCAACTTGGATTTGCATACAATAAAACAACTAAAAATCTTGAATTTTATTTTGAAAAATTAGTTAATAACAAATGAAAAATTTTTAGCTTATCAAACTTTTTTGATAACTTAAGAAAAAAATTCGACCGAACTAACCCAGTCGATGAAGAATTCACAAAAGATAAATTAATTACATATGATGCATTTCCATCTGATTGAACATTTGATTTACCTAACACTGTTACAAAAGATGATGTAGATAAAATGTCTATTTACTTAGAAAATGGTGTTAAAGAACTTAAATTTACATTGACAAAAATAAAACAATATTCTAATATCAAATAATCAAAAATAAATTAGGCCTTGCGCCTAATTTTTTATTTAGCCCTGTTTTATAGTGCTTTTGGTAAAAAAAGCAAACTTATTTATTCTTCTTCTTTATCATCATTTTCATCACTTAAATCTTCAATCATAGGCATATTCATTGCAACTTTATCAATGTCATAATCTTCTTTTATAATACCTTTTTTTCGTGCACTATCAATAGAATTTTGAATTCTTTTCTTTACTCTATCGACTATTTTTAATGCTCTATTTAATTCAACTCCTGTTTTATCATTTTTCTTGATACTTTGTTCAATTTTGGTATAAATATTATCTTCACTTTGACGAATAAATAAGAATGCATCTAAAAGAATGTCAATATTTTGTTTTATGTTAATTCCATAATTTGCATAAGCAATGGTTTGAATAATCATCAAAATTGTACTTGGTCCAGCCAACATAATATCATGATCTTTATTCAATGCTGAAACCAATTCATTGTCACTAACTGCTAAAGCAAAAATAGCTTCAGATGGTAGATACATAATTGCTTGCTTGGTAGTTACATTTTTAATAATGTATTTACTTTTAATACTTTTAGCTTGTTCGAGAATAACTTTTCTAAAGTTAATTTGAGCTTTAATTCTTTCTTCTTTAGAGTCTGCATCTTGATATGCATTAAAAGAATCAAGTGGAAACTTACAATCAATAGGCAAGTAACGATCATTTTGAGAGTTAGCTTCTCCATTAGGCATTTTAATAACAAAGTCAACTCTTTCGCTATTTGGGTTCATATCTTCAAAATCAACAGCAGCAGCTTCATCTAATCCTTTTTTAGCAATTTTCTTTAAATCAACTTGTGCAGCTCACAAGTCTTTTGATAAATGTTCATTCAAAATACTCTTTAAATTAAACTCACCATAATTACCGCGACTTTTTGTTCCATCAAAAGTTTTTTGTAATGAATTAACTGAATCTTGTAAGGTCTCAAAACGTACTAAATTCTTGTCAAGATTTTCCATGTTTTCTTTAATATTTTTAAAGTTTTCTTCCATCTTTTTAGCTACTTCATTTTCAAATTTTTCATCAATAGCACCTTTAATTATTTGCACTTCCTTAATTACTAAATCATCAAACGATTTCATTTTTTTGTCAATTTCTTCTCTTAAATCACTAAAGTTTTTATTAATCATGTTGAATGAATTGGCAATTTTTTCATCAACATTTTTATTGAATTTGACAAATTGACTAGACAAATCTTCAGTTATTTGATTTTTAATATTATTTGTCATTTTGTCTTGATCGATAACTTCTGTCTTATTTTTTTTATATAAGATGAAACTAACGACAATACTACTAATAGCTAAAATCATAGTCAATATTACTACAATTAGAATTCCTATTTCCATATTTTTTCTCCTTTAATTAATTGGTTTTTTAAATTATATAATTTTTAATTATATTTTGATTAAAATAACAACTTAAAAGCTCAAAAATTGCCACAATAAATATTATTTTTTTGTTTTTGCAAGACTCAAAAAGTTTTACAATTTAAAATTGTATTGATTTTAAAATCTAGAAAGAATTTATGAAAATAGTTAAATTAACTAAAAAAGAATATAAAGGTAAAGAATTCTGTGTCCAATATAAAACAAAAGGCTATTACTTTATAGAACATACTAAAAATGGATTTAGTTTTGTTTACAAAAAATTTGATCAAGAAAAAACTTTTGGTTACTGTGACAAGTTTTTTCAAGATTGATTAAATAAACCAATTTGCTATGGTGCTTTAGATGACCATAATAAGTTGCTAGGTTTTGCTGAAGGTTATTTAGAAGAGTGAAATAATCGTTTTAGAATTACTAACATTTTAGTATTTGATGAAAATAGTAGACATAGTGGTATTGGTTCTAAATTAATGAAAAAAATGATTGCAAAAGCTAAAACAACACCTGCTAGAATGATTGTTTTAGAAACTGAAACATGTAATGAAAAAGCAATAAGATTTTATCAAAAATTCAATTTTGAAGTTATTGGATTTGACTTGTATGCATATACAAATGAAGATCCAAAAGAACATGAAGTTAGAGTTGAAATGGGTTTAAAAATCTAGAAATTTTGCTATATAATAGAAATATAGTTTTTGGAGGTAAGATATGAAATTATTAGTTTTAACTCATCCAATGTTTAATGACATTGAATTAACAACAGTCCTTGGTTGTTTAATTAGAAGTGGCAAAATGAGCAAAATTACTCTTTATAATCACAAGCATAAAAGTGCTACAGGACAATTTGGAGTAACTACTTTGGCTTTAGAAAATAAAGTTAATTTAGATGAATACGATGCTATCTTTATTCCAGGCGGTAAAGGCGCACAAGAATTAAGAAAAGATCCTGAAAGTTTAAAAGTAATAGATTACTTTATTAAAAATGATAAATGAGTTTGTGCTATTTGTGATGCGCCTAATGTTCTTTATGAAAATCATTTTATTGATGATAAGGTTAAATACAGTTCATTTCCAATTATACCTTTAGTTGGCGGTAAAAATAGAAATGAAAAAATGGTAACAGTAAATAACAAAATTATTACTGGTCGTTGTGCAGCAAGTTCAATGGAATTTGGTTTAACTTTAGTTAAAGAATTCTTTGGTGAAGATGTTTACGAGCCTATTCGTAAAGGAATGTTTGGAGCTTAAAAAGGCAATAAAAAAATCAGCTTTTGAAAGTTGATTTTTTATTATTCTTCTTTTTCAATTTTATGCTTTTGAGCCTTTTTTATAAGCACAAAGAGCAAAATTTGAAGTTTCAACTTATCTCATCAAACAGTGTTCGAAATGTTTTCGAAATTGCAACACATTTGATATTCAATAACCAATTTACCTTTAACATCATCTCAGTAAAAAATCATTTGATGTTTCTTATTGTTAATTAATTTAAAAAGCATGTATTTATGATCATTTCATACTCGATATGCATTAAAAGCTGTAAATCATCTTTTGTAATTTAATGAGAAATATTTAATTAAATTTTCTAGTTTATCAAAAGAATATTTGTATGATTTTTTGCTAATTTTAATCTTGCCAACTCTTTCAGCTAATTGATTGTATTTGAATGAAAGTAAGTTGTTTCGATTTGTATAACTATTTAGCATTCAAATTAAACAAATGAAAAAGTAATAGTTATTAAATTGAGTTGAATAACGAGGATTAGCTATAAATTTGTTATCAATATAAGCAAATAAACAGCTTTCATTTTTTAATTGTTTATTTATATTTTTTAAATCATAGAAATAATATTTAACTTTATATTGTTTTAATAATTCTAATATTTGATAAGTAGTATTTTGTGGAACAACAACATAAGAATTACTAATATCAATTCCATTACGTTTGATTTCTTCTAAAAAGAAATCTAAATAAATTAATACTAGTTCATGAGAATTAAGTCATTTAAATTTGTCATTTAAACGCAAGCAAACATTTAAGTTGCTGTTGATATCAAAATGAAAAATATTTTGAATTTTGTTTTCACGATATCATGAGCTAGCTGCAAAAAATGTTAATCATTTTAGGTTTAAAAGCGAAATATTTTTATTGTTTGTAATGTATTTATTTTGATAATTATTTAATAGTTCTGTACCAATACTTTGACTAAATTTATTGTCACTAGCAAAGTAACTTAATGTTTTATATCTTTGTTTAACATTAACGAAAGCTTTAAGAATTTGTTCTTTTGAAGCTAATTCATCAATCAGTTCAAATTCATTAACTAGTTTAGGATCTTTGATGACAATTTCTTTATTTTGCCATTTGTTTAATTGATAATAATCATAAATTTTTTGTAAGTTTTCAGAGGTCAAAATTTCAAAGTTTTTATCAAATATTTGAATTGTAATATAGTTATCATTTTTAATATTTTTAAATTTGATTAAAAAGTCATATGAAGCTTTTTTATAGGCATATTCATCTAAAATCAAACTAATTGAAAAGTTATTGTGATACTTAAAAACATTATGATTATTTTTGATAAAATAAGAGCTAAAATATTGAATATTTTCATTATAAAATTCTTGACCTTCAAAGCTTAATAAAATGTTACTATTGCTTTTTAAAATAGTTTTATTTATAGCTTGCAATAGTCGCAAGATTGATGAGAGTTCTATTTTTTCTTTGTTATTTGTTTTAAATAAAAAGGTATCATTGCGAATTAAAAGATTATCGTTTGAAACGATAATTTGCTCTTTTAATTGTTGATTATTTGTTTGCATAAAATTACCTATTATTTATATTAAAGATTTTTGATTATTCTTCATTGTTTGAATCAATATCAATTTCATCGTTTGAGTTATCATCTAAGTTGATTTCTTGACTTTGTTCATAAATGCTTTGAACAACAGAAGTTGCAGCCATAGTTGCTTCTGGATCTGTTTTTTCATCAATTTTAATGATTTCAACAGCTTGAATTTCTTCACGCTTTTTAAGGTTAATTATTTTAACACCTTTAGCTGCCCGACCAGTTTGGCTAATTTGGCTAATAGCTGTTCTAATTGTTAAACCACTTGTTGTAATAATAAAAGTTCATCTTGAGGATTTACGAATCTAGCAAAAACAATGTCGCCTGCTAATTTGCTATTAATTCCTTTAACACCTTTACCACCACGTTTAATTAATCTAAATAGTGAGTGGTGAGTAAGTTTTCCAAAACCTTTTTTACCAATAGTTAAGATTAATTCACCTTCTTGACTTGTTGAAGTGCTTGAAACTGTTTGATGGCTATCAAGTGTTATACCTTTAACACCAGTTGCAGTTCTACTAATTGGTCTAAAGTCAATAGCTTGGAATTTAACAATTCTTTCTTCATCATTTGCTAAAAGAATTAAGTCTTCATCAGTAATAATCATTGCTCTAATTAATTCATCGCCTTCACGTAAGTTGAAAGCATATTTACCATTAGCGTTAATTCTTTCGTATTCAGTTAATCTAGTTTTCTTGATAATACCATTTTTAGTTGCTGTAAATAAGTAGTGATCTTCATCGTAAGTGTCTACTGGAAGCATTGAAACAATTCTTTCACCTTCTTTAACACGAAGTGAAGGAATAATATTAATGAATGCTACACCTTTACTTTGACGAGATAATTCTGGAATTTCATGAGCTCTAACACGGTAGACACGAGCATGATTTGAGAATAATAGTAAGTCGGTGTGAGTTGTAGTTGTTAATATTGAGGCAATATCATCATCGTCATATGTTTTCATAGTACCAATACCAACTCCACCACGACGTTGAGTTTTGTATTCTTCTAAGTCCATTCTTTTTACAAAGCCTTTGGCGCTGGTTGTAATAACAATGTCACGTTGAGGAATCAAGTCTTCATCAGTAATAGTACCTTGAATTTCATAGTTAATTTGTGTGCGACGTTTGTCATTGTATTTTTCTTTAATTTCAGTTAATTCTTTAACAATTAATTTAATTAATTCACTATGGTTAGCCAAAATAAATTCAATTCTTTCAATTTCTTTTCTTAACTCTGCCATTTCTTCAAGCATGTTTTCAATAGCAAGTCCTGTAAGACGACCTAAACGCATGTCAACAATAGCTTTTGTTTGTTTTTCAGTTAAATTAAATCTTTTTGCTAAGTTAGCTTGAGCTTCACCATCTGTTTTAGATTTCTTAATAATTGCAATAACTTCATCAATATTTTCAACTGCAATTTTAAGACCTTCTAAAATGTGAAGACGGTCTTGAGCTTTAATTAAATCAAATTTTAATCTTCTAGTCACTACTTCTTCTTGGTGTCTTAAGTAAACTTCTAAAGCTTGTTTTAAGTTTAAAACTTTAGGTTCACCATCAACTAAAGCAACAAAGTTAACATTGTAGTTTACTTGTAAGTTTGTTTGACGATAAAGCTTGTTTAATAAAACATGAGGATTAACATTCTTTTTAACGTCAATAACAATTCTAATACCTTCACGGCTACTTTCATCACGTAAGTCGGCGATACCTTCGATTTGTTTATCTTTAACTAATTCAGCTATTTTTTCAATAATTGCAGTTTTCTTAACTTCATAAGGAATTTCAGTAACAATAATTTTGCTTTTACCATTTGAGTATTCTTCTATTTTAGCTATTGATCTGATTGGAATGGATCCCTTGCCTGTTAAGTATGCATCTTTAATACCTGCATTGCCTAAAATCATAGCTCCAGTAGGAAAGTCTGGACCAGGTAAAAATTCCATTAAGCCATAAATATCAATTTCTGGATTGTTAGCTAAAGCAATAGTTGCATCAATAGTTTCACCTAAGTTGTGAGGTGGAATACTTGTTGCCATACCAACAGCAATACCACTACCACCTGAAACTAAAAGGTTAGGGAATCTTGATGGTAATACTGAAGGTTCTTTTTCACTAGCATCATAGTTATCAACAAAATCTACTGTATCTTTTTTAATACCTTCAAGCATTTCAGCAGCTAATTTGCTCATTCTTGCTTCAGTATAACGCATCGCAGCAGCTTCATCACCATCAATAGATCCAAAGTTACCGTGACCATCAACTAAAGGATAACGCATACTAAAATCTTGCGCCATACGAACCATAGCTTCATAAACTGAACTGTCGCCATGTGGGTGGTACTTACCTAAAACGTCCCCGACAATACGAGCGCTTTTTCTATGTTGCGATCCAAATGTAATTCCTAATTCATTCATATCAAAAAGAATACGTCTATGTACTGGTTTAAGTCCATCACGAGCATCTGGTAAAGCACGAGAAACAATAACACTCATTGCATATTCTAAGAATGAGTTTTTCATTTCTTTGTGAACTTCAACTGGATGAATACCATCTTTAGGTGCATCTAAAAGTTGTGGTTGAACTACATAGTTGTCTTTATTTTGAGGAACTTCTTCTTCCTCCCCTTCAATAACTTTAGTTTCTTCTTTTTGTGGTTTAAAAGCAACAAAAAGCTCTTCATCAGGAATGTAATCTTCATCTAATTCTTCGATTGCATTTTCTTTTTCTTCTTCTTGATTAAGTTTGCTTTTGTATTCTTTTTCTTTATCTTGTTCTTTTGAACTCATAAAATACCTCCTTCAAACTTTTAATATATAAATATATTAAAATATTATAGCATTAAAATGCAGTTTTTTTAATAAATTTTTTATTTTTTTATTTTCATTTTTTATGGCTTTTTTACATTAAAATTTTAAAACTATATTTTTACTAAATTAGCTGTTTTATAGGCTTTTAATAGAAAAATCAATATAAATTAAGAGAAAAAATTAAAAGCTAATTATGTTTGCTTTAATTAAAATAAAATATAATTAAATAGTTTAAATTATAGAAAGGATTAAAAAATGGAAAATATAAACACAGAAAAACATAAAAAAATGGACTTCAAAAGTTGATCAAAATTGAGAAAATCAACATTTATTGTGGGTATTATTGTTCTAGCAATTTGTGTGTTAACTTCTACTTTAAGTTGAGTTAATATCTCAATGACAATTAATAGAATGATAACGAAATTTGATGAAGAAAATCCAGGTATTTTAGTCAAAATGCAAAAGGCTCATATTGCTCCATCTTTTTTAGCTTATATTTGAAAAAAAACTCAAACTTTTACTTATATTTCTAATTATTTTTTAGCTATAAGTATGATCCTTTTTGGATACTTTTATCACAATAAAAGAATTCTAAAGACTTTCTTTTTAAGTGTAGTTTACATTAGTATTACTTTTGCTATTTATTGAACTTTAATTTTTCCAAAAATATTTGGATCAGAGTATGGTTCCTTTAGATTAGCTTTAAGCACAATTGTACATGCAATTAATCCATTAATTGGAATAGTCATATTGATTTTAAATCGTAAAAATATTACACTTGATAAAAAAGTTATGTACATGTCTAATTTAGGTGTTATTGGTTATTTATTATTTGCCTTTGGCACTTTTTGAATTGGACTAAGTTATTCACAAAACTACAAAAATGATGTAGCAAATAAACTAAATCAATATGTGGATAGTGATATTGTAGTTTATGGCTTTTTAAACTTTATTGAACCATTTTTTTATCGCGGAGGTAATATTGCAATAGTTATTACATTAGACTTATTAATGATTATAATAGCAGTCTTTGTACCTATTGGCTTAAGTTATTTTTGAAAAGCAGTATTAAGAATAAAAATTGAAAAAAATAATGAAAAAGATAGTTTATTAGAAACGAAAAGTGATGAAAAAATAGCAGAAGATATTAATTAAAAAAATATTAGGTTTTTAAAACTTTTAATATAAAATTAAAGAGCATATAACAGCAAGTATTCATAAAAGAAAGATAATAAAGTTTCAGATTCGATTTGAAAGTCGGTTTGCTGATTAAACCAAAATCATTAGAACTTTATTAATTCATTTAGAGAATACTCTCTTCTTGTTTTATGCAGAAAGAGGAAACTTATGAGATATAGAGGTCCCGTTTTCAAGAAAAGCCGTCGTTATGGATTTTCAATTTTAGAAACAGGTAAAGAATTTTCAAAAGGTAGAAAAAGAACTTATGCTCCAGGTCAACATGGTGACAAACGTGTTAAATTAAGTGACTATGGACTTCACCTTTATGAAAAACAAAAAGTTAAATACTTATATGGTTTAAATGAAAAACAAATGCACAAATACTACATTCGTGCAACCAAAATTAAAGGTGTTACAGGTACAGTATTGTTACAACTTTTAGAAAGTCGTTTAGACAACTTAGTTTATAGAGCAGGTTTTGCTTCAACACGTCGTCAAGCACGTCAATTAGTAAGCCACGGCCACTTTACATTAAATGGTCACAAAGCCGACATTCCTTCAATGAATGTTGAAGTTGGCTCAAAAATCCAATTAAAAGAAAAATCCCAAAAGAATAAAATTATTGAAGCTAACCTTAAAGATCGTTCAGCTGCTAAATGATTAACAATCAAAGGTTTTGAAGTGTCATATGACCGTTTACCAGACAGAAGTGAATTACATCCAGAAATTAAAGAAAACTTTATCGTTGAGTTCTACTCAAAATAATAATTAAAGGAGTAAGCTTATGAAAAAAAATATTCATCCACAATATTTTACAGTTGAAGCAACATGTTCAACATGTGGTAAAAAATTTCAATTTGGTTCAACTAAAAAATCAATTTCTGTCGATGTATGTTCAGGTTGCCACGTAGTTTATACAGGCGACAGAACAAAAACAAAAGCAACTGGTATGATTGACAAATTCAACCAACGTTTAGCAAAACAAAAAGCTGCTATTGAAAAATCAAAAGGTAAGAAAAAATAATTTTACTAATAAGAGTTTTTAATACTCTTATTTTTTATTTTTGGCACTTAAAAGATTTATTGACATATAAATTTATTTAAAAAATTACCTAAAGCACTATAAAATAGGTCTTTAAAACACAAAGTTAATTTAGCACTCTTTTTAATAATTACTATTAATATTATTAAATTAAGATATAATTAAATTATGGCTAATATAAATTTAAATGACGAAAAAAAGAAAATTCTTAAATATGTTATTGAATCTTACATTGAAGAAGGGATCCCGATTGGTTCTCAATTATTGGTTGACAAATATAAATTGAACATTTCTTCATCCAAAGTTAGATACATTTTAAATGAATTTGAAGAAGCTGGACTTTTAGAAAAAACACACACTTCAAGTGGACGTATTCCTTCAAATCTTGGTTATAACTATTATGTAAAAAATCTAGTAAATAAAGATTCAAACATACTATATGAAAAATTAAAAGATATTTTTGCTCGCCGTCGTGTTTCAATTGATCAAACAGTTAAAGAAGCGGCAAAAACCATCTGTGAAACAGTTGGAATAACACTTGTTACTTCAGAAAGTAATGAAGATGCAACACTTAAAAGTATTCAACTTGTACCACTTAATACTGCTGAAGCAACTGTTATTTTAGTAACCTCTTATGGTGAAGTTACATCTAAAACAATAACAATTGACTTAAACCTTGTAACAATGTCAGATTTGAAAATAGCTATTAGAATTTTCAAAGAAAGATTAATCGATGTACCAATTTTAAAACTTAAAAGTACAGCAGAAGCATTGACCCCAATTCTTGCTGATACAATTAAAAACTATGAAAGTATTTTGCAAAGTTTTGTAACAAATGTTTTTGATTTCCAAATCAAGAATGAAAATGTGATTTATGGAAAAGATAATATAATTTTAGCAGACGATATTTCAAGACAAGATTTAAGTAAAATACTTCATTTAATCGAAACTAAATCAATATGACAAACAATTGAAAGTGAAATTGATGAAGACGAAAATATAAAAATAGCAATTTGTGATGACCACAGTACCTTTATTAGTAAGAAACTAGAAACACAAAGCAAAATTAAAGAAATAGCACTTGTCGGCACTAGCCGAATGGATTATGAAAAAGGATTTAGTGCTCTTAAAGCCTTAGAGGAATTTATTGAAAATAAACCATGCAAATCAAAAGATGAAGGAGATAAATAATGAAAGTAGTTCCTAATTTAAACAAATTTAAAACTGGTGAAGGTATTATTGCAAATATATCAGTTTATAATAGCTCAAATGCTTATCAAAAACAATTATCAAAAGAAAAAGTAAGCTTTGTTTTAGGTGAAAATACATTTTTACCAAACTTTGACAACAATATTGTTGAACAAACTGTTAGACCAGTTTTAGATTTTGTTGTTACATTTCCAAAAAGCTATGAAGTAGCAGAATTCAAAGACAAAACATTTAGATTTGTTATTGAAATTTTAGACTACCAAGATAGTGAATATGTTAAAGCATACAAACAAATGTTAGCTTTAAGAGAAGAAATACAAAAATTAAATGTTGCTTCAGGCAATCATTTAAAAGAACTTGAAGAATTAAAAGCTAAAAATGAAAAACAAATGAGTGAAATTAGTAATTTAAAAACTGAAAATGATAAACATACTAAAGATTCTGCTGATCTTAAAAATCAATATCAAGAACAAGAAAAAACTATTCAAAAACTTATTGAAGATAACAAAAAATTTATAGCAGACAATAATGCTTTAAAAGAAGATAATGAAAACAAAGGTAAACAAATTAAAGAATTAATGTCAGGAAAAACCACAAAATTAATGGAGCAAACTAGTGAACATGAAGCAAAAGGTGTTAATCCTGCAGTTTTAAGAAAACAAGTTGATGTTTTAAGAAAACAACTTGAAGAACAATCAATTGAATTAAAAGAACTTAGAAAAAATAAAGCTCCTGAAGCTGTTCAAAGAGTGACGGTTCCTAAAGAAGAAGTTGACAAAATTAAACAATATGCATTACAAAAATTCTTCGAAGATTTTTCAACTCATTATACAACTTTAAAAGGTGCTGTTAAAGCTGGTGCTAAATCAGAAAACAGTAGTGTTAAAAACTATGTAGTTGGTTTTGAAATGATTTTAAACTTAATCAATGGCGTTTTAGAAAAACACTGTATTAAAGCAATTGAACCAAAAATTGGTGATGAATTTGATCCTAATACTCAAAAAGTTCTTGAAGTTGAAGAATGCAAAGATAAAAAACACAATACAATTGTTAAAGTTAGTGCTATTGGATTTAAATTACATGATAGAGTTATTAAACCTGCTTTAGTTGTAATTGCTCAAGATTCAAGTGCAAAAGACAAAAAACTAGCAGCGGAAACTAAAAAACCAGAAGCTCCAAAGGCTAAAGAAACAACTAAGAAAACTGAAACTAAAAAAACAACAACCAAAAAATAATTCAAAATAGGTCAGTGATTAAAGTCACTACTTATTTTTTATTAAAACAACTATAAAATAGTTATTTAAGTAAATACAACTTTAATTTTTTTAATTATTTTTCAATTTTTTTATGAAAATAGTATTCAAATATATTAAGCACTAATAAAAATAATATATAATTAGATTATGTTTAAAAAATCATGTAAAGATGCTTGAATAGTTGTCAAACGTGGCAATATGTTCATGTTAGCAATTGGTCTTTTATTAGGTACCGCATTTAATGCTGTTGTTTCATCTTTAGCAAATGATGTTATTATGGCAGCAATTGCGAAAGCATTCAATGTGGATGAAGTTAAAGACTTAAAAGCTGGCCCAATTTTAATTGGCAAATTCTTAGCTGCTTTAATTTCATTTTTGATTGTGGCAACAGTTATATTTATATTGTTAGTTGTTGTCTTTTTAGTTAAAAATGCTATAGAAACTAGAAAATTTAAAAAGAACCCACCTGTTGCGGAAGAACCAAAACCTACAACTGAAGAACTTATTTTAGCTGAAATTAAAAAACTAAATGAAAGACTTGAAAAACTAGAACAACCTAAAAAATAAAACAAGAAATTGCAAAGTGTAGAGTGAACCCCAAATTCCGAACTTTTTGGAAAGGGGTTCATTTTTATGTAATTTTAATTTAAAAAAAGAAACAAATGAAATAGATATATAAATGGTTATTTTTGACTTATTTAACTATAATAAAGGACTATACGGTTATAGACGTATTACATTTGTTTTAAGAAATAAAGAAGTAATGATTAATTATAAAAAGGATTAATAAAAGCATTAAATCTTTTTGGCAAAACATTAAGAAAAAAATAAGTATTCTTCATTCAAAGGTGATGCTCATAGAAATATTCCAAACTTGCTTTTAGATAAAGAAAATATCACAGAAGATTTCTTCAGATACAAAAGAAATTTTTCTAATGAAAAATATTTAGATATATGAGGAACAGATGTTACCGAATTTAAGTTAATAAATGAAGAAAAAATATATTTTTCTCCTGTTATGAATTTTAGGACCAGAAAGATTTTAGGTTATTCAATTACTAAATCTCCAAATTTAAGAATGATAGGAAAAATGTTAGAAAATGTTGAAGAAAATGGTCATAGTTTAAACAATGTATTAATACATTCTGATCAAGGATGACAATACACTCATCAAGATTATATTGATTATTTGAAAGAAAAAAACGCAATTTAAAGCATGCAAGAAAAGGAAATTGTTTAGACAATAGTCCTACCAAGTGTTTATTTGGTGTTATAAAAATAGAATTTTGATTTGGAAGAAAAGAAATTTAATAGTTTTAAAGAATTTAAAACCACTTTAGAAGAATATATTTCATATTATAACAATGATAGAATTGTTAATGAATTAAAAGGACTTAGCCTTGTTCAATACAGGAATAAGTCCAAACATAATTAAAAAGTCCAATGTTTGAGGTTCATACTAGTGTAGCTTTGTAATTTTTTTACCTTTTTTCACAAATTTTTCCATTTTCGCCTTTATTTATTACCAAAAAGAAAAGTAAAAAATGAAAGGAGGACTATGAAAGACAAACAAATTCAATTAAAACCTTTAACAGAAGAACAAGAAGCAAACATTCATGGTGGATCAATTATTGCTACCATTTCAGGAATTGTTGGCATTTTAGCAACAATAGCTACAACAGTAAATGGAATTGTTAAAAGTCATAAATCACCTAAAGGCGAATTTAAAGACAAAACAGGAAGTCAAATTAAATGAGATAATTCTCCAGCAACAAATGGAAATATCTTTTATGCGTGATAAAAAAGAGCAGGGCAAAAGCCTTGTTTTTGCTTTTTATTTAGCTAAAATTATTCAAAAAATATTTTGTTCATTATTTTAATAATGTGGTAAAATGAATAGGCAATATTTAATTGCAGAAAGAAGAATCTAATTCTCACCTGATGGCCAATAGCCTTGGGTTTATGCTACAATTAAAACCTAATTTTAAGTTTTATGTAGTAGAGAGTGGGAAACCACTTTTACTTTTATTTTTAAAAATTTATCTATAAGGAGTTTATTATTCAACCACAAGGAAAAATGAAAAAACCAACAGCAGAACACATGATTAATGAAAATATTCCATTTCAAAAAGTGTTTGTAGTTGGCGCAAATGGTGAAAAAGTTGGTGTTTTATCAACACGTGAGGCCATTGAAATGGCCAAAGAACAAAGATTAGATTTAGTTCTAATTAGTGTTCAACCTAAACCAATTGCCCGTATTTTAGATTATGGAAAATTTAAATACGAAAGAAAAAAGAAACAAAAAGCAGCTAAAGAAAAACAAACTGTAATTCAAAATCGTCAAATTAGACTTACTCCACTTATTGGAGAACATGACTTAAATGTTAAAGCAAGAAAAGCTAAAGAATTCTTATTAGATGGTGATAGAATTAAAGTAAGTTTGAAATTTAGAGGTCGTGAATCGGCTCGTCCTGAACTAGGTCACAAAACTTTAGAAAAATTTTACAAAGCTGTAGAAGACTTAGCTGATATTGCTAAAGAAGCAGAATTAGTTAATGATCGTTTCTTAGATATGTATTTGCAACCAAATAAAATCAAGGTTAATAAATATAGAAAAGAACATAACTTAGTAGATAAAAATTCAGATAGTCAAGATAAATGTGCTTCAGAAGAAGACACAAATGAAGGAGAGCAATAAAATGCCTAAAATGAAGACTAAGAGTGCTCTTAAGAAACGTATTAAGATCACTGGAACTGGAAAAATTATGCGTGAACAAGCGTATCGTTCTCACTTAAGCCAAAATAAAACAACTAAACAAAAACGTCAAGCTCGTAAATCAGTACAAATGCACTCATCAGATGTAAAAAGATTTAAAGCATTAATCTAATGCTTAACTTGAAGTTATTTAATTTATAATGAAAGGAAATTATCATGAGAGTTAAAGGCGGAACAGTTACAAGAGCAAGACGTAAAAAATGACTAAAATTAGCAAAAGGATATTTTGGCCACAAATCAATCGGTTACAAAGTTGCAAAACAAGCAGTTGTTAAATCATGAACATATGCTTTTAGAGACCGTAAACAAATTAAACGTGAATTCAGAAAATTATGAATCGCACGTATCAATGCAGCAACTAGAGCTGAAGGTTTATCATATTCAAAATTTATTAATGGATTAAAAAGAGCTAATGTTACAATTAACCGCAAAATGCTTTCAGAATTAGCTATTAGCGAACCTAAAACATTTGCAATGTTAATTAAAATAGCTCGTGATGCAAAATAATAATATTAAAATTCTATTTAAAATGAACCACAAATTCCGGACTTTTGGAAAGGGTTCATTTTTATACAATTTAAATTTAGAAAATAAAAAAGGAAGAAAATCTAAAATAAATTTAGACGCTCAAAAGGCAACAAATTTTAAAATCAATATTGAAAATAAAATAGAAAATAAGGATTTATTAATTAAACAATTAAAAGAAGAAAACAAAATACTCAAATTGGAAAACGCGATAGCAAAAAAAGTGAGTGCCTTGCTTCAATTGAAAGACTCACTAACAAAGAAAAATTCTAAATAACAATTGAACTAAAACAAGAATTTAAAAAGCTATTTTCTATTAAATCAATACTAGAAAAAATCAAATTGAAAAAGTCAACTTTTTATAAGATATTAAAATCAAAAAATAAACCTGACAAAGATAAAAATTTGAAAAAGATTATATTTGACTTATTTGACTATAATAAAGGGCTATACGGTTATAGACGTATTACTTTTGCTTTAAGAAATAAAGGAATAATAATCAATCATAAAAAAGTGTCGCTTATAAATGCGAGATATACTATTAAGGATTTTTTATTTATGAAAAGAAAACCAATAAATCCTGTTATTGATGAAATAACAGAAAAAATTTTAGAAAATTATAATCCAGTTACTTCTGGTGATCTTAGTATGGCTATGAAAGAAGTATTTCAAAATACAATCCAAAAAATGATGAACAAAGAATTTGATAATTTTATGGGTTATGAAAAAAATGATAATAAAGTTCAAAAAGAAAATTATAGAAACGGTTTTTCTAAGAAAAATGTTAATAGTCAATATGGTCAAATGGAAATTGACATTCCAAGAGATCGTGAAGCGAAATTTGAACCTATAATCATCAAAAAGTATGAACGTGATATTTCTGAATTAGTTGATATGGTATTTGCTTTATATTCTAGAGGAATGTCAACAAGAGATGTAAGTGATTTTATGTTTAGCAAATATGGCGTTAATTATTCTCCAACACAAATAAGCCAATTAACTAATGAAATTGTTGAAGATGCAAGATTATGACAAGAAAGAAAACTTGAAACATATTATCCAATAATTTATATTGATGCAGTACATTTTCATATTGTAGACAATAATGTTGTAACTAAGAAAGCTACATATGTTATTATGGGCATTAATGGTGATGGACAAAAAGAAATTCTAGGACTTTATATTCGAGAAAATGAATCTGCAAAGTTTTGAATGTCTGTCCTAAATGCTCTTAAAAATAGAGGAATTAGTAAAATTGATATTATTTGCTCACAGATAATTTAAAAGGTATACAACAAGCTATAGAAGCTGTTTTTCCTAATAGTAAGCAACAACGTTGCATTGTACATATGATTAGAAATTCTGTTAAATATGTTAACTACAAGGATATGAAAGAATTTTGCAAAGATTTAAAATCTGTATATCAATCTAATGATGCAAAAAATGCAATGGATAATTTGGATATTTTTGAAGATAAATGAGGCAAAAAATATCCTACATCTATTTCAATATGAAGACGGAACTGATCTGAAATTTCTACAATGTTTGATTATTCTCTTGAAATAAGAACGCTCATTTATACCACTAATTCAATCGAAAATTTAAATAGCGTATTTAGAAAATATACAAAAACAAAAACGGTTTTCCCATCTGATGATAGTCTTTTAAAAATTCTTTTTCTAGCATCTCAACAAATCATAAAAAAATGAAGTAATTCAAGAATTAGAAATTGATCTAGCATTTTAAATGAATTTAAAATTATTGATTTTGAAAATGAAGAATAATTTTTTTAAATTTTAAAATTCAAATTTTAAAAATAAGATAATGTTTTTTTAGGCATTATCTTATTTTATTCTGCAATATTATTAGGTGTTGATCTAAAGTTAATTCCTTTCTATTGAGAAAGGGAGAACTTTACACAAAATTCTAAACAATCTTTATTTTTTTAATTCAAATATTAAATTTCATTAATTTATCAAATAAGTTTGCTGTATGTATTATTACTTGTTGCAGTAAAGATATTTGATCATTTATCTCAAATTGAAACTGTTTCAGAAGACGAATTATTTTTCAAAATGTATTTCTCAGTGATTGCAAATTCTGATTTTCAGTACATTCTATTTCATATGTAGTATGAAGCATTAAAGTTTCCTAATGGGTTATCAAGAGTAAATTTAAAGATGTAATTTACTCATGAAATAGGTTCACCATTATGTTTTAATAAGAAAGTAGTTGAAGCATGATCTACTGTTCTTAAATATGAGAATAATGTATTAGAAAATGGCATATATTCAAATAGTGGGTTAGCACTATCATATTTTTCATTTGAATTGTAAGCAGATGATCTATAGTAAGGACCATTGAAATCACTTTCAGTTGGATTTCCATCTGGACCTGGAAGAGTGGGGAATGGTTCTTCTTCGCCATGAACTCAAACTCTATTTCTTAATGGATTATTTCCAATTGCGTAATGAGATTTGTTTTCTTTTCCACTTCATCTTTCTCATATTCCTTCATAATATCCATTGCTTGTTGGATTATTGTATTTAATGAAATCGAAGGCATATTTTATTTTGTAAAGAGCATCATTTAAATTAACAAAATTGCTTTGAAAATCATTTTTAAACATTATTTTAGAATCTGATGTTTTTTTGATATTTGAAAGCATTTTTTCAGTTACTGTCTTCTCATATTCTATTAATTTGGCTAAAGTATATAGATCATCATGTCCACTTCAAATATCATTTTGTATTGCTTCTAAATAATCACGTGAATTCATATTAGGATATTTTTGATTTATATATTTAACTGCTGGTGTGTTACCAAAAAGCCTACGGTTAATAATTGTATGTCAAGGCACATAATATTCATTTTCAATTTGTTCACCTTGTTCAATTTCACTAGTACTATTTGTTATATCTACTCCATATCGGTGTCATGAATATTCGGCTCTAACTTTATCACCTTTAGAATTTCCTCAGCCTCCATTTGTGTTACCTTTTGAGTATCCTTCAGTATCTAAATCTGAAATATGGTCATATAGATGTTTTTTGCCTAGAGCTTTAGGTGTAGTTCAAATTTTTGATTTTTCATCTCAATTTGCATTATTTTCTGAGACAGACGTTGGTCTATACAATTTTGTTGGAGATCAGTATACATCACCATAATTGAATTTTTCATGTAATAAGTAAATTAGTTTGTTTAAGAAATATTCTCTTAAATCTTGAACGGCTAATAAATAATCATTTTCTAAATAATTTTTAATTGAATTAATTTTTGCAAAATCAACGCCACCATCAAGTTGTTGCAAAGCTTTTAAACCTTCACTATCTAATTTAGAAATTTGATCATTTTTAATTGCATTCATTTCATTTTTTGCAAAAACAAGTGAACTTTCTTGTTCTGAAGTTAATTTTGCTTTTTGTTCAGTTTTTGCTTTTTCTTTTACATATTCATTATAGTAATTAAGAACAGCAAATCATTTTTTAACAAAGTTAACTTTGTTGTCTTTATCAGCTTGAATAACACTAAAGTTTTCTGTAGAATTAACATTTTTATAAATTTCAATTGCAGCATCAAAAATTTTGTTTTTATATTCATATACTTTAGAATCTACATTTGCTATTGCTGCTAATTTTGCATCCATTTCATTGTATGATTCTAAGCAAAATTGAGCTTCATTTTCTAAGAAATTACTATAAAGTTTATCTTTTTCATTAATTGATGAAATAAAACCATTGATTAAATTAATTTTTTCAAATAGTCCTTTAAATTTTTCTTTGTCATTTTGAATTTCACTGTTTTCAATTGAAGATATTTCTTTAGTTGATAAATCTGGTTTGTTATTATTAATATCTTCAATTGTTTTTTTAGCATCTTCGAATTGGTTTAATGTTTTTAAATTAGCAAAAACATTTGATAAGAAATCAGCAATTTTTTTATCTGCATTGCTAATAAGTTTTGAAATTTCCTTAGGTTTTGAAGCAAAGTTATTAACTTTTTGTAAGTTTAAATCAGTTGCATTTTTAATAATTTCGAATATATCTAAATAATTTGTTTCATATTCTTTAAGATTTGAAATTACTTCATAGAATGTTGAAATATTATTAATTTTGTTATCAATATCAGAATCTAAATTAGCTTTTAATAAAGGATCTAGTTTTGTTTGTATATCAATTATTGATTTATAAGAAGTTTCGAATAAATTAAATTCATTTAATGTTGTTTTGTTTGCTTCTAATGCTAAATTAAATTCATTTAATCTTGATTTAATATTAACAAAGGCTTTTTTAAATCCATTTTTTATTGAATCTAATTCATTTTTGCTATTAACATAATCATTAATTAATTTGTTTCTACTTTCAATATACTCATCTCATTTAAAAGAACTCAATTCAATTTTAGATTTTTGTACAAATTGATCAATAATTTGTTTTGAGTTATTTAGTTTAGCTAATCTAATTGTTGTTTAAATTGAATCAACATTTGAAATATAAGCATTTAAAAATTCTTTAATAACATACTGAATAGAAGTTGTCAAATCATTTTCAGCGTTTAGAATAAATTGAATTTTGTTAATAAATTCATCTAATTTTTCAAAAGAAATTTGATTTGAACTTTCAATAAATGAGTTAAATTCATTAAGAGCATTAACAATAGGCGTTAATGATTTGCTGCTTAAGTCTAAAATTTGAGATTTGTTAAATAATTTTGTACTTTCAATTGTTTTGACAAAAGTGTTAACATAACGATTTATATATTCCTTTGTATTTCCATATGATTGTTCTAATGTTTTACATGCCTTAATAATATCTTTTATAATAACACTTCTATTTACTAAAACACCACCATTTTCTAAAATAACGTCATAATTATTAGAGTCAAATCTTGATAATAAAATCTTTTTAACAGCATTAATAGGATTTAAAATTGGAGAATTATTACTTGCAATAGTATTAGCATCTTCTATTTTTGCTGATAATTCTTCTATTTTATCTATGTTTGACATGAATTTTTTAATTAAGCCGTCTTTTTTAACATATTTTTTAATAATTTTGTCATTTCAAACATGAAAATCAGATTTAATAAAATCTGCTGCCTTATTAATCATTTCAGTTGAAATGTTAGGTTTTGTGTTACTTGAAAGTGCTTCTTCTTCGATTTTACTTAAAGTGTTTTGATAATCTTTAATTTTAAGTTCTAAAGAATTTAATTCACTTTTACTATTAGCATCAACAAATTTTTCATCTGCAGTTTTTAAAGTTAATAAATCATTACTACGAACAACAAAATCTTGGATTGATTTTTTGATATTTTCATATTCGCTTTGATTAATAACTTGTTTTGTTCTTTCTACCATTTCTGGTAAAAGATTTTGAATTATTTTCTTTTCATTACTATGTTTAACAGTTCTCATATAACTTTTGTTACCAAAGTGGTGACCAAGATGATATGAAGAGTGTTGATCAATAATTCCTTGTAAATCTTTAATAAGTTTTTCATTTGAGTCAATTTGAGTTTGACACAAACTTAGTTCATTTCAAAGACTCGAAAGTCATGCTTCATTCTCATATTTCTTTCAAATTTCTTTTAATTTGTTGTCTTTTTCTTTTAATTTTTGATTTGATTCTTTAAGGTATTTAATATTGTTTTCAATTCACAACTTTTCTTTTTTATAGTTTGAATGGTCAACTGCATAACCAATTAAAGTACCGGCTGATACTGCACCAACAATCCCTGCAGAAATACCCATTGTTAAAGCTACAATTTTTTTTGTGCTATTTTTCATAGTTGTCTCCTTATATTTAATATAAAAAATTAAATTTTTGGGACATAAGGAAAATTATGTCATTATATTAAAATATTTATTGGACACTTTAAAGGTTAAAAAACATGAAAAAGAATAATAATTTTCTCGATATTTTTTTGAATAATATCTACTCAATGGTTCAATAAATCAAAGAAGTTATCAAACTTTTAAACAATATAAAAGGTACTTATCATCCTTAAAAAAATGAAACAATGTGAATCAATTAATTATAAGGATGAATCGTGAATTAGCAAATAATAAAATTTCAAATAATACTAAATTAACTAAAAGAAACATATTTTCTTCTTTTTTAAATTGATTTTGCAAAATCACTAATACAAATACAGATGTTAAAAATGAACTTCTATATATAAAAGAAGAAAAAGACAAAAGACAAGCTATACTTGAAAAGGAATTCATTAAGCTTGTTGAAGAATTAAAAAGCCACAAAAACACTAAATTTAGTATCATTTTTCGTGTGTTTATTTCAACTGGTATTAGAGTTGGATAATATAAAGAATTTATTAAAAACATTGATAAATTAGAAAAAAATTGAAGTTTTATTATTAAAACATTGAAAAACAACAATCCTATATACTTTTGGATTTCATCAAAATTAGAGATGCATTTAATAGTTGTTAATTTCAAAAGTGAAATCTCTAAAACCTAGGGGTCTCACTTTTGAAATTAACAAATTTTTAAAATAAGATAATGTTTTTAGGCATTATCTTATTTTGTTCAACAATATTATTAGGTGTTGATGTAAAGTTAATTCCTTTTTATTGAGAAATGGAGAAACTTTACACAAAATTCTAAACAGTCTCAAATTAATAGATAATATTATTAATTAGCATTTAAATTTTGAGTTTCCGTTTGTTCTTTAGTTTCAATTTCTTGAGTTAAAATTGATTCTCATTCCTTTGTTGATTCTTCAAAATCTTTAACTGGAACTTCTTCTCTTTCAAGATAATCACTTTGTTTAATTTTATCGCCTTCTTCGATTAATTGATCAAGTGTTTTTTCTTGTTCTTCAATTGCATTTTCAGCTTCAATATTTTTTGTTTCTCCCTGTTTTATAGTGTTTTCAGTCGAATGTGAAACACTTTGAATTTGTTCATTTTGTTCTTCAACATTAAGCTCAATAACCATAGTTGCTTCATCTTTAATAAAACCAACTTCTTGAGTTTTTTCTGCATTCAAAGTAATTGTGTCTTGAGCTGTTTCTTCTTTTTTGATAGCTTCAACAACTTTTGTATTTTCTTTAGATTTTTCAATTTCTTGAGTTTTGGTGTTTTCACGATCTTGGCTAATTTTTTCTAAAACTTCTTCAATAGTTTCATCTTTCTTTTCATTATCTAAAACTGGTACTTCTTTAATTTCTTCAACTATAATAGTATCTGAGTGTTCTTGACTGATAAAGTCTTCTTCATTAACGTTAATTACTTGACTATTTTCATTAATTATTATTGCTTTAACTTGATTTTCTTCTTCAATAACTTCATTTGCTTCTTTAATTTCTTGGTTTGTTGCTTCAAGAATTTCTAATTGAGTTGTTGGTATTTCTTTAGTTACTTCAACTACTTGAGTCATATCAACTGGTTCAATACTATTAATAGTTTCAAGAATTTCTTCTTTTTTATCTTCTCATAAAATTCGATCTAGTTTATAAACAATATATTTTTTAGATCTAATAAATTTACACAAGTCTTTAAAAATAACAAAGTCTTCAGGATTATTAACATATTTGTAATCATCGATCATAAATGTCATTATTGGTTCATGTTTTCTTCTTAAAACATAATCAACTTTAAGTGTTCCAAGTGTTTCATTTGGAATTAATTTTAGATAAGGTTTATCTTTAATTGCTTCAAGAATTGTGCTTTTGATTTCTTGATTTAATAATGAATTCTCAACTTCAACTGGTTCAGTTTCAATGGTTTGCAATTGATTATTATTTTGTTCTTCATCTTCTAAGTGTAAGAAGTTTTTTCTTTCAATGTCACTTAATTCTAAGAATTCAAGTCAATGTTTGAACATAATAGCATCTTCATTACCTGTCATTATAGTTAAGTCACTAGATTTAAGTGATTTAATAACAATCATTTTATCCTTAGCACGACTAACTGCCACATTTAACGCATTCATACCGCCAGGACGTCCAACATAAGTTGAGTGAATTGCTGTATCTTTATCATAAGCTACTGAAGCTATAACTAAATCAGCTTCATCGCCTTGAATATTTTCAATGTTTCTTAATAACAAGTTTCCATTATTAATTGCTTTTTCTAATTCAGGATGATTTTTGAAAATGCTTGTGGTAATTGAATCTTGTTGTTTAGCATTGAAGCACAACAAAATGATTTTGTTGTATTTATTAATATTTTCTTTAATAAGTTTGATTGCTAAATCAGCTTCAACTGTGTTTTGATTATTTTCTCAAAGGCCATTAACTTCATGTACTTCAATTGGCTTGAAATTAGCTTCCATATTAGCTGAATCGATAACATCCAAAGTACTTTTGTAGAAGTATTTAGAGCTAAAAGTCATCAGTGAAGCATAATTTGAACGATAGTTTTTGTCTAAAAGCACACTATAAACTCCAAGACTTTTAGCAAAGTCTAGAAGGCTATCTACTTTTCCATAAATGCTTTCGTCATCACTAACTCTGACACCAAATCAGTTACTTGGTTTCATTTGTTGATCATCACCAGCAAGAATTTTAATTTTTGCTAAATATAATACTGGTAAACCTTTTTCAAGGAAAATTTGGCTTGATTCATCTAGGATTGCATAGTCAAATTCGCCTTTATTTCAGGCACTTAAGTCAGCTTCAGGTGTCACAATTACAATAGGGAAAATCTTTTTAATCATATTAGGAAAACGCTTAATAAACTTGTAAGGTTCTAATGTTCCTAATCTAATTGTGGCTGCAAATTCTGAGTATTGATCTTTTTCTTCATCAGTAAATTTGTTAATTCGTTTAATAATACCTTGAGCAATAAATTTCTTTAATTCATTTTCATCGCTAGTTTCTTGAGTATTATTAGAAGGCAAAATGTTTATTAAATCATTTAAAAATTCAAAGTCTTCTTGCTTTAAGTTTTTTAAAACTTGAACTGAATCATTTAAATTTCCTTGAAATTCTTTAAATGTGTTAAACATTTTTTGGGCTGTTAATTTAATTAATCAGTTTTTGAAATGTCAGAATTTGAATTTCATTTTGTTAATTCTAAGCATGTCATTAGTTAAAGCTTTTGAATCATCAAAGTGAAATTCGGCTGGATATAAAATATTTTTAGGCAATTTTAAAATTGCATTTCAGATTTCTTGTGAAAATGATGATCAATTATTATGAACTTTAGCTAAAGCTTGAGTGATATTAACAAAACGTGGATCACTACTGAAATTAGCTATATTGTTAACAAAACGAATATCTTTTTGGTTAATGATTCTTAATGGTTTTAAATTAGGATTATCATCATAGTTTTCTAAATAATCTAAATATTCTCTAAGAGGCAAGTAGAAATTCTTTTTGTTCATGTTTTTTGAATTAAGCATGAAAAGACAAAAGATTTTTAAAGCGCCCATTCTATTTTTAATAACTTCAAGAGCCGCTTTCTTTTGAGAACAAACTAAAGAATTTTTGCCATAAACTAAAAGGTTAGTCAAAATATTAACAATAGTTTGGCTTTTTCCTGTACCTGGAGGACCTCAAATAATTGTGTTTTGATTTAAGCTTGAAGCTATAGCTTTATCTTGAGAATAGTTGGTAGGTGTAATTTTAAAAATAGATGTTTTAGGGTTGTAAATTGTTTGTTTTACCCTATTTTTATAGATGTTTTTGTTAAATTCTATATCTAAAATTTTGTTCAATTCTTTATTCTTAATTATTTCAAGCATTCTATTTCTGGCATAGCCACCACTTGGTTGGAATAAGCCTAAAACTACGCCTGGTTCAAATTCCATCGCTTGGTTGCCAATAGCTTCGGGAATTCAACGTTTGAAAGGTTGATTTAAGTCTAAGTTAAAGTTGTAAATATCTTTTCATTCTTCATTTAAATATTTAATTAATTCTTTAATTGATCAATCACCATAGTTTTCCGAGATATTTAAATCAAAACCTGCATTACTTAATAAGAATAGCAATTTTTCATTAATTTTAATGTCACCATTTGAAACTAAATATGGTCTAGCATTTCTAATTTCTAAATAAACTTCTTTTAAAAATAAAGGACCATAAACAGCTTTATCGTCAATAACTAATTTAACAAATAAGAAACCTAAGTGCATAGGTCAAATGTTAGTTTCAGTGTTAATTTCATTAGCTTTATTTAAGAATAACTTTCATTTTAATGATTGTTTTTGAAAATTAACTTTTAATTCATTAATAATGTTATTTTTAATTCCAAGAGGATTTTTCTTTAATTGACTATTACGTTTTTCACTTACTTCAATATTGTTTTCTTCAAGAATTTTAATAATTTCTTCATATGTATTTGCTTCTTCAAGTAAATTACAAATTCTGATCAAATTAACTTCAAGAATAGGTATGTCAAAGTTTTTATTGTTGTAAATTTTGCTAAAACTTTCAGGTCCAAAAATAGTATAAAAATCAAAAGCAAATTTGTTATTTACTCTAGTAAATACTGCACTGTCATTTGGACTAACATCTAGTAAATTGTCTAGAATACTTTGATATTGTTTTTCTTCATTGTTTGACATGATATTGACCACCTCATCTATTTATATATGTACATTAATTATAAATATAAAACTTAATATTAATAATAAATCATTGTTTGAAAAAGTGCATTATTTATAATTATTTTTTTCAAAAATCAAGTGCAAAATAAAAAAGTGGCATAACCACTCTTATTTTTATTAATATCAAAATTATTGTTGTTCAGCATTATAAGAATTTGTTGTTTCTCAAACATTTTTCTTTTCTTTACCTTTAACGCTTTCAATAATAATACCAACAATAGAAACTACCACAACACCAATGTGTGTAAAGAATTGGATAAATGGTATTACTCCACCGATGAATTTTTCATCGCTTTCTTTTGTCATTTTGAATGTTCCACCAAAGAATTGGAATACTAATCAAACTAACATTGTTACAAATACAGCAAGCATAATTGGAGCTATAAATCAGTTTTTGTTTACGAATGCTTTTATTGTAACTGCTAACACTGTAAAGATTCATGAAATATAAATGATAAAATACAATGCTGAGATTCCACAAGCTATATAAACCATTGTGTCTGATTCAACTACGCTTTTAATTATTGAAAAATTACTTGTGAAGTTTGAAAAGTCTGCAAAAATTCCATTTGTTACAAGTCTGTTTGCTATTAAAAACTTACCTGCATCTCCACTTTTTTCACTTACTTTTGCCATTCAACTTCCTTTAGCAACAATAATTAATAAAACAGTAAATATTGCTAAAATAATTGACAAAGTAATGACAGAAGCGTATTTAATTTTTTTGCCTTTATCTGACATAAACACCTCATTTTTTAATCAAATTTTATAGTTTTTAATATATAAAACTATGTAATTATTATACAACTTTTCTTGTTTTTTGCTATGTAAATTATTTAATATTTTTTTAGGTTGTAAAATGATCTAATTTCACTAAAACAATTGTTTTATAGTGCTTTAGCTTGCAAGCAGGTCCTTAAATTGTTTATATAATTCATAAATTCTTTGCTCATATTTAGTAGTTTCAAAGGGCAATATTTTTTGAGCATTTGCTCATAAAACAACGATATAATTAACCAATATTTTGTGTCTTAAAAGATAAACTAAATTATATTCGCCATAGGTATCTAAAAATAATTTTTCTTGTTTTTTGTCTAACTCATTTGCTTCAATATAATAAGCAAGTTCAAAATGTTTATCACCTAGCGAAGCATATTCTCAGTCAATAAAATATACTTTTTTATTTTTAACTATCATGTTAAAAAATCAGAGATCATTATGCAGAGGAGTATCTTTTCTCATTTTTGAAAGTGTCATATTAATAAATTTATAAAAATCATTCAACACTTTAATTTTAAGTTTCTTTTCTTGAATAATTTTGCGATATTCTTTAATTCTAGCTGCATGATTAGAAGCAGGAAAATTAAGTTTTGAATTGTGTAAAATTTTTAAATTTTGAGCTACTTCCTTTATATTATTATCATCATTTCAGTATTCTTTACCATCAATATATTCTCAAGTGATTTCTTTGTCATTTTGGCTAATAAGTTTTGGTGTAAAACTAAAATCTTTTAATAAACTGTATTCTAATTTGTGATTAAATTTATTTAAAATTTTTTCTTGAATAAACTTATTTTCATCTCGATATGAAATGTTAGTATGTCCTTTTTTAATTAAAACTTTCATTTTTACTCTTTTTTTATTTCAAAAATTATATATTATATTATTATTTATTTAATATAAAATTTAGCTTACTTAAATAGGTGAAGATAATGGACAAAAGCAAAATTAGAAATTTTTCAATTATTGCTCACATAGATCATGGTAAAAGTACGCTTGCTGATAGAATTCTAGAAATTACTAATACAGTTAGTGACCATGATATGGATGATCAATTTTTGGACCAAATGGATTTAGAACGTGAAAGAGGAATCACAATTAAATTAAATGCTGTACAAATTAAGTATAAAGATTACGTATTCCATTTAATTGATACACCAGGTCACGTTGATTTTACTTATGAAGTTTCACGTTCATTAGCTGCTAGTGAAGGAGCATTATTATTAGTTGATGCTACTCAAGGAATTGAAGCTCAAACTTTAGCTAATGTTTATTTAGCTCTTGAAAATAATTTAACTATTATTCCTATTATTAATAAAATTGATTTACCTTCAGCTGATATTGAAGCCACAAAAAATGAAATAGAAGAAGTGATTGGTCTACCAACTGATAATGCAGTTTGCATTTCAGCTAAAACTGGTTTAAATTGTGAAGCAGTTTTAGCTGCAATTGAAAAATATATTCCAGCACCTAAAGATGCTGATGATTCTAAACCTCTTAAAGCTTTAATTTTTGACTCGTACTTCGATGAATATCGTGGTGTAGTTATGCTTATAAGAATTTTTGAAGGTGAATTAAAAGTTGGCGATGAATTTATGTTTATGTCGAATGGACAAAGATATTTTGTTAGTGAACTTGGAGTTAGAAGTCCAAAAGAAACTAAGAAAAAGAGACTTGAAGCAGGTGAAGTTGGATATGTAGCAGCTACTATTAGAGATGCTAGAGAAGTAAGCGTTGGAGATACTATAACTTTAGTTAATAGACCAGCTGAAAAAGCACTGCCAGGTTACAAAAAGAAAAAACCTGTGCTTTATACTGGGTTTTATCCAATTGATACTCGCGACTATATGGAACTTAAAGAAAGTTTAGAAAAAATAGCTCTTTCAGATAGCTCAATAACTTGAGAACAAGAAACTTCTAAAGCTTTAGGATTTGGTTTTAGAGTTGGATTCCTTGGAATGTTACATATGGAGATATTGCAAGAGAGATTAAATCGTGAATATAAAATCGGTATTATAGCAACTAGTCCTTCAGTTGAATATGATGTTCATATGACAGATGGCAGCATTAAAAAAATATCAAACCCTGCACTTTTACCTGATCGAACTTTTATCGATCATATTGATGAACCTTACATTAAAGCTGAGATTTTGGTACCTAATGAATACATAGGAAATGTTATGGAACTATGTCAAAATAAACGTGGAATTTATATCAATATGGACTATATTGATGCTAATAGATCAAGGCTTATTTATGAAATGCCACTAGGCGAAATTATCTTTGATTTCTTTGATAGACTTAAGAGTCTAACTAAAGGTTATGCATCATTTGAATATGACCTAATAGGCTATAAAACAAGTGATTTGGTAAAAGTAGACATACTTTTAAATGGTGATAAAATTGATGCTTTTTCAATTATTACTCATAAAGATTCAGCTTATAGTAAAGCTCGTGATTTAACTCAAAAATTAAAAGAAGCTATACCAAGACAAAACTTTGAAGTACCTGTTCAAGCAACTATTGGAAGTAAAATTATTGCTAGAGAAACTATTAAAGCTTATCGTAAAGATGTTACTTCAAAACTTCATGCAGCTGATGTTTCAAGATATAAAAAACTTTTAGAAAAGCAAAAAGCGGGTAAGAAAAAAATGAAAATGCTTGGTACTGTTGAAGTTCCTCAAGAAGCATTTTTATCAATTCTTAAAACTAATATTGATAAGAAAAAATAGTAAGGGATTTAGAGTGAACCCCAAATTCCGGACTTTTTGGGGAAGTGATAGAAAGAAGTGTGTTGAACACTTTTTTTATTTCACTTTTGATTATTTATAAAAGTACTCATAAACATTAATTCATATTTTTATTTAGTTTCGTATTCTGCTAAATTAATATACTTTTCAAAAAAATGATCAAAAGTATAAAAAAATAACAGCGTCTAGAACACTTACTACCCGTTCGACCTGTTGTATATATTATACCACAGTCGCTGTTATATGTTCAAAAAAAAGGAGAAATTATGAGTGTATACGGCGAACTAAGGTTAATTGCAAATGAAGGAAGCGAAAACGAAGTTAGAAAGTTTGAAGCTAACTTGGAATGGAGAAAAAATTATTTTGGCAAGAAGGTTTATGACAAGTTAAGCCAAGATACAGTTAGTGAAATTTTAAAAACAAAGATAGTACTCGGTGAAAGCTACTATAAGATTTTGAGAACTGAAAAAGTTGCTTTTGAAGTGTATGTCTGTTTAAGGTTTCTTGGGGCTAAAAAACGCTATGTAAATTTTTATGAATTAGTGGCTTTTGGCTTCAAGAAAACAAGCTTGCAAAGAGCGGTAAAGTTTTTAACAGATATAGGGCTAATTTTAAAAGTTAGAAACGCTGTGAAAATTAAGAAGTTTAAGCTAACTAATGACGATAGAAAGTTCATTGTTATTAGTGGTGATAAAGACTGGAAGATTTTCTTGTTGTTTGGATTAGCTAATTTATGAGCTTATAAAACGCTTGTATGAAAATCAAAAGAACTTGGAACTAAAAAGTTTGTTAAAAGTAGAAAGATGAAAGTCATTGTACAAAATAACTTTTTAGGGCTAAAAGGTTCTACGGCTTACAGATTCTTGAAAAATATATGTTTGGTTTTAGGTTTGAGAACTGATGAGTTATTTATTATTCAAAGAAGTGTTGATAACTTGAAGCATTTAAGCTTTAAGACTCAAAGGTATCTTGTAATGCAGATATAAAGCAAATGAGGAGTAGTAATGAATAAGCTTCAATTATTACTGCGGTTTATTCATTACTGCTTGCTTTAGAAAATCTTATGGACGCACACTCGTGTGTTATCTTTCATATTTAACCTTTTTGGGGTTTAAAGACATTTTAGAATAAGTATGCTGATGATGTCCAAATTAGGTCGATAAATAATTAGGAGTATTAATGAATGTAATGAATTAATACGAAGGAGAAAGAAATTAAATACAAAGAAGGAAGTTAGTTAGTTAAAGAGGAGGGAGAATGGATGTTTATATGAAGTTCAACTATGTATCTAAAATGACTAACTATAAAACTGGTTTAGGCTATTGTCATGATTTAAATAGTTGAGTATCAAGCGGGGCAGTTATTGATTATTTTGCAAGAGGAGATAAGTGTGTAGCATTTAGTCCTTTTGATGATAGTGAAAAAGATAGAATGCTTTTAGAGGAAGTAAGTAGAGAAGAAAAGATTAACTGATATAGAAATCATCTTAAGTCTAAAATGGGTAGCCAATATTCTGGTGTTTATGATATGTTAAACGATTCTAGTGCTGATGTAAATATCGATAACGTTAAGGAAGAATTAAAGAGTATTGATGAACAAACATTTTATGAAGGCATTTTAAACTTAGGTGATTTAGGTTTAGAACATAATGTTTTAACTGCTAATGATTGAGCTGAGTTAGTTAAAGAGGAGTTTAGAAACTTCTATAGCATTAGCGGTTTTGATTTAGATAACCTTAGTTCTTATTATTCAATACATGGTAATACAGATAATCCACACATCCATTTTATGTTTTTTGAAAAGACTAAAAAAAGAGCAAAAAGAAAATTAAACATCGACGATATAAAAATGCTAAGAATGAAGATTACAAACAAGCTTATTAAAAACTTTGATTATCAAAATCTTAAGGATTTAACAAGTAAGTTATGAGATGCCAGAAAAGATGTTTTATCTTCGACAGAACAAACGCTTGAGAATTTTCAAAGCCAAGATTACAAAGACTTTATTGAGGCTTGCTGTTATGTAAGAGCTGAAATGGAGAATAGGTTCAATAAGTCTTATAAACTCGCTTCACCAGAAGTTAAAGAAGCGATAAACAAGATAGAAACATTTTTATATAAATATGATGATGATTTTAAAGGAAAGATGGATAACTATCAAAAGGCATTTGATTTAGTTATGCAACTAAAGGATGAAACGAATTCAACGTTTTTAAAAAAGAGAATCAATAATGTTTTGAGAACTGAAAAAGATGAGTTTTCTAGACAGCTAGGGAACAAGATAATAAAAGCAATTCTAAAATCAAGAAATGCTAAGAGCGCTAAAAGCTTTACAAAATTATTCTTTTATAACTTGAATGTAAATGATGATTCTTGAAGATTGATTAATAGAGCTAAGAAGATTTTTGAAGCTCAAAGCATTCAAGAATACGAAAACATTATGACTTATTACAAAATATAGGATTTTATTATGGGAAACAATATATCTAAGATAGTTAAAAAAATTATAGAAACGGAAGATTGAAATGATGTTATAACGGGTATCAATTTACTTCATTATAAAAAGATTTTGAAAAAACATATGCCATTAAGAAACGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCATTTACTGTTAATGTGCAATTTTTTTGAAGCTCCGGATTTATAAAAAGATATAATCATAGAAACTACCTTGAATTCATTGGTAGTAATCATCAAAAATGTTTTGATATGGTTATTTTCATTTGGATAGAGTGAATAAAAAAATCAATGCCTTATCAGCAAAATTTAAATACTTTTTACTCTTTCACATTTTTTCAAATAAAGATTTTGAACTAATCTTTTTGAACAAAGATTCAGTTTTAGTTTCAGAAATTTTAGAATTTGCCGTTTTCTCTGGTAATAATTTTTTATTAATTATTATTTGTTTTTTTTCTGATTCTAACTTGTCATAATTTAATCCATAGTTTTGAAAGAATGTTGAAAAAGTATCAAAATTTCCTGTATAAGACATAATTTCACCTCCTTTCTATATTTAGTTTGATAGAAAGAAGAAAAAAACAAGCTTTATGCTTGTTCTCTCATTTAAATTCATTCCCACAATTAGTTTACAGCACCGACCTGTTTTTTAAATTAATCTGTTTTATAGTTGTTTTGGTAAAATACCAGTTTATTCAAGTTTTGTTCCATCAGCTAAATCTATGGCTACAAAAATGTCTTTAATTTTACTTTTGCCAATTGTATATCTAGTTATTTCTTGTAAAACATTAACAGCTTCTTGACATCTTCTTTTAGCATCTTCAGCATAGTTAAAACCAGCATTTAAAAAGTTGTCATCTTGGATTTTGTTAATAAAATTAGTAAAATCATTTTTTGCTTTTTCTCATATTTCTGCTAATTTTTTATGACCGCTTTTTTTAAGTTCTTCTTCAATAACTAGAACATCTTTTTCAAAGTTTTCTTTAATTTCTTTTCTTAAAGTTAATATGACATTGTATAACTCATAGATCATATTTCCAATTGCATGTGAGTGATTATGACCACTGTTATTACTTGAACTACTATAATTTGAAACTAATTCTTGATTGCCTTTTTCTTTTCAAGCTTTTGGATGTAAATTAGCAATAATATTTGAAATTGATTTATAGTCCGTACTCAAAACAGTTTCTCAGTCTTCATTTAAAATTTGTTTATTTTTTTCAAAATATTTATGCTTAAGAATATAGAATCTACTTCTTTGTTGTTGATTTTCATCGTCTTCTGTTAAACCATCTAATAATCTTGAATCATTGAATATTTGAGCGTTAACATCTAAAGCTAAATAAAGCATTTTATCGAAGAATTGGTAAAATTTGTCTTTTCCACTTTGACTTGTAAAAAAGCTCAATTTGTCTTTAAAATTAACTGCTGATTCAACTTCTTTCATTAAAGTATTTAATTCAACATTATCACTATTTATTATTCCGGTTTGACTGCTGCCAATTATCATATCCATCATGTCACATATTTGAGCTATGTTTTCTTGATTTTTCGATAATGTCATATCTAATGATTTGTCATACAAAACATTATTGTCATATTCAAAAGACAAATTAATTCTAGCTTTATTGATTAAGTCTAAATATTTTTCGATTTTATCGACAACTGGATTATTATGAATATTTTTTTTTGATCATTTATTTGTTGAATTTAATGTGCAACCCGCTGAAATTATTAAGTTTGGTGCTATTATTGTAGGCAACAAATAAAGTAATATTTTTTTTCTTTTGATGAATGATTTATTCATAAAAACTCATCTCTACACTAAAAATTAGTATTTTTTATACATTTATTATATATTAATCACTCAAAAAAACGCAAAAAAGAAATTTTTGCCTTTGAAGGTGTTAAAATATAGTTGCAGCCTTGAATAAGCTGTCTACACAAGCACAGGTTCGAATTTTATAAAAAAATTAGAAATGAGCTGTGTATTATAATTCGATTTAATTTTTCTTTTTTCCCTTAAAGAAAAATTGAAACAAGCGTGCGGAGTATGCTATTTAATTTACAAAATTATTTATCATTTTTTTAATACCCTTTCTATTCTATTTTTTCATCACATTTACTTACCTTTATTTTTTCTGTCTATTTTTTTATGTAAAGGTTAAAAATTTAAAACACGCTTCTCTGCACTTTAGTGTTTTAAACAAAATATTATATTAATAATAATGCAGGTTAACGCCTGTTTTTTTATTATATAATTTAACAAATTAATAAATAAGGAGAGCTATGAATGCTTTAGATATTAAAAAGAAAATTATTGAATTAAGTAATAAATTAAACCAATGAAATAAAGAATATTATCAAGATAATAATCCTAGTGTTAGCGATTTTGAATATGATAAAGCTTTACAAGAATTAGAACAATTAGAAAAAAAATATCCTCAATTTATTTATGTTAATAGTCCTACTCAAAAATTAGGATCTTTTGCTGATAATAAATTTAAAAAAGTAGTTCATCAAAAACCAATGCTTTCTTTATCTAAAGCATATGACTATAGTGAAATTGAAAAATATGTCAATAATATTGAAAAATTAGTTCCAATTGAAGACATCAATTTTTCAATTGAGCCTAAAATTGATGGTCTTTCAATTTCACTTCATTATAATCATGGCTTCTTAAACCAAGCTTTAACAAGAGGTGATGGTCATGAGGGTGAAGATGTAACTGAAAATATTTATCAAATAGCTTCAATACCAAAAATAATTAATTATGATGCTGAATTAGAAGTTAGAGGTGAAGTATTTTTACCTAAAGCTGAGTTTCAAAGATTAAATGAAAATTTAATTAAAGCAGGCGAAAAACCTTTTTCAAATCCAAGAAATGCAGCAAGTGGGACTTTAAGACAATTAGATCCTACTATTGTTAAAAATAGACAACTTGCTTCCTTTTTATATGAATTAGTTGAGCCTCAAAAACATAATATTACCACTCAAGAACAAGCTTTAGAATTCTTTCAAAAATTAAATATACCCACTAATCCATATCATAAAGTAGTTGAAGTTGAAGAATTGGAAGAAGCAATTAGTTTTTTTGCTGAAATGAAAAATAAACTCAATTATGATGCTGATGGTTTAGTAATTAAGCTAAATAATTTAACCTATTGAAATAAAATGGGTAAAACTGCTAAGTTTCCAAAACATTCAATTGCTTTTAAATATGAAGTTGAAACAGCAATTAGTACTATTAAAGATATTAAAACCACAGTGGGTCGTACTGGCAAAATTACTTATGTTGCAAGTTTAGAACCCGTTGAATTAAATCAAACAATAGTTAGAGCAGCAACTCTTCATAATTATAATTTTATTGAAGACTTAAACATTAACATTAATGATCAAGTTCAAATAGTTAAGGCTGGTGAAATAATTCCTAAAGTTTTAGCTTTAGTTAAAAAACAGTCTAAAGGTGTTTTTGGTAAAACTTTAGAATGTCCTTCTTGTGGCAGTGTATTAGTCGAAATTGAAGATAATGTTGATCAATTTTGTGTTAACAAAAATTGTCCTGAAATGACAATTAATGCAATTTATCACTTTGCTTCAAGAAAATCGTTAAATATTGTAGGTTTAGGATTAATCACAGTTAAAGATTTCTATAAACACAATTTAGTTAAAAGCATTGAAGATATTTTTAATTTACATAAACATAAAGATGAATTATTAAAACTTGAACGTTATGCAGAGTTAAAAGTTAACAATTTATTAACTAATATTGGAAAAGCAAAAGACTCGACTTTTAGTAAGGTTTTATTTGCTCTTGGTATTAAACATGTGGGTGAAAGAGCTGCAAAATTAATTTCAAAACAATATCATAATTTTGACGAATTAATTTGTGAAAAAGATGAATTGGCAAAAATATCAGCTATAAATAATATTGGTCCTAAAATTTTAGATAGCTTAAAAGAATATCTAAAAAATGAAGAAAATGTTAAACTTTTAAGAAAATTTGATGAGATATTTAATTATCAAAAAATTGCTACAGTTGCTAGTGAAAAATTAATTAATTTATCATTTGTTATCACAGGTAAATTATCAAACTCAAGGGATCACTATGTTGATTTAGTTGAAGCTAATGGAGGCAAAGTATCTTCATCAGTTTCTTCTAAAACGTCATTTCTTTTAGTAGGAGAAGATGCAGGTAGCAAATTAGATAAAGCTAAAAAATTAAATATTGCAATAATAAATGAAGAACAATTTAATGAAATGTTAAAATAGTTATTATTTTCATAATATTTAAAATAATTTTTGAGTATTTAACTAAAATACTTTGAGTTTTTATATAATTTAATCAATTATTTAGGAGTTATTATGGATATAAGAAAATATGTTGTTATTGGTAACTGAAAAATGAACAAAAATTTTACAGAAACCATGGACTTTATTTTATCTTTTAAAGATGAATACAAAAAATTAAAGAAAAACAAAGAAGCATATGCAATTAGTCAACTAAATGAATTTGCTGTTGCAATACCTCATGCTAATTTATCAGCTTATCAAATTAACAAAGTTAATGAATTAAAACTATGTGCTCAAGATGTTAGTCAAAATTGCAATGGTGCTTTTACAGGTGATGTTAGCGCTTCAATGCTACAAGATCTTAATGTTAACTATGTAATTCTAGGTCACTCAGAAAGAAGACAATATCATCACGAAACAAATGAGTTAGTTAACTTAAAAGCTAAACAAGCTCTTGAATACAATTTGACACCAGTTATTTGTGTTGGTGAAACATTAGAACAATATGAAAAAGGTCAAACAAAAGCAATTGTTAAAAAACAAATTGAAGAAAGTTTAAAAGATTTAGATTATTCAAAAATTATAGTTGCTTATGAACCAATTTGAGCTATTGGAACAGGTAAAGTTGCAACTCCTGAAATTGCTCAAGATGTTTGCCAATACATTCACTCAATTACAAGTAAAGAATTAGTTGTTCAATATGGCGGTAGTGTAAATCCTAAAAATATTAGCGATTTAGCAGCACAAAAAGACATCAACGGATTCTTAGTTGGTGGTGCAAGTTTAAATGTTGATTCATTTATTAAACTTATTACTTTAGGCAAATAATTTAGATTAAAAACAGCCCTCTTTGCAAGGACTTTTTTAATTGCGATTTTGTATTAAAACAACTGTTTTATAGTGTTATTAGTGAAATTATTAAATTTTATTTTTGATTAATTTCGCTGATAATTCATCAACAATAATTGTTACATCTTTATGATTTTGTAAAAAGCTGGCTGGACAATTTGAATTTACTGGTTTAAGTAAAGTATCATAAATTGCTTGAGCTTTTTTAGGCCCGGTTGCTAATAACATAATTTTTTTACTTTTTAAAATAGTTCCTATTCCCATAGTTACTGCTTTACGAGGAACATCTTTTTCACTAGCAAAAAATCTTTTGTTTGCATTAATTGTGCTTTCAGTTAAATCAACTACTCTTGTAATATCTTGTTCTTGACTATCTGGTTCATTAAATGCAATATGACCGTTTTCGCCAATACCTAAAATAGTAAAATCAATACCTCCAAGTTTTTCAATTACTTGTTCATAGCTACCATTTTGATGTTCTTTTTCATATTTTATGACATCAGGAAAATTGATATTTTCTCTTTTAACATTAATGTGATTAAACAAATTATGATCCATAAAATAATGATAAGAATGTTCATTTTTTAAATCTAATCCAAGGTATTCATCCAAGTTAAAAGTAACCATTTTACTAAAATCAATTTTACCTTTTTGATAATCTTCAATTAATAATTTATATAAATCTAAAGGTGTTGAACCTGTGGCAAAGCAAATTCTTGAATCTGCTTTTTTAGTAATTTGATTCTTAATTAAACTTAATGCTTTTTGTGCTACTGCTAAAGCATCTTTTTCAATATATACTTTCATTTTTACTCCTTATTATTTGACTTTTTGCCCATCAATATAAACTTCTTTAATATCTAAAGTTTTTAAATCCATTAAAACTAAATCTGCTAATTTATTTGCTTTAATTTGTGCTCGATCTTTTAAATTATGAGCTTTAGCTGCATTATATGAAGTTAATTTAACAATATCACGATAATCTAAATCTAATGAAGCTAAATTTTTAAATGAATCATGTAAGCAAATTCCACTTCCAGCTATAGTTGTTGTGCCCTCTAAATAAATTGTTAAACCATGTTTTTCAATATTAATTCCACCTGAAATGCTTTTACCATCAGGTCCATAAGCAGGTCGAATTGCATCGCTAACACACATTAAGTGGTTTATATCTTTATGATTAAGCGTAAAAACTATACTTTCTTGACAAATGTGATGAAAATCAACAATTAATTCACTATAAACATCATGTCTTTCTAATGAAGCTTCTAAGATTCCCGGTTTTCTTGAATCAATTCCACTCATAGCATTTCATAAGTGACAAGTATTAGTTGAACCCAAATCATAGTATTTCATAGCTTGATCATAGCTTGCAGCTGAGTGACCAATTGAAGGCATAATGTCATTTTGAACCATAAAAGGAACTAATTTTTCTTCAACCATTAAAGGATCATAACTAATTTTTTTCAATTGATGATTTGAACTATTTAGCATTTCTAAAATATCTTCTCTAGTTGCTCTTCTTAATCATTCTAATTTGTGAGCTCCTTTTTTAGCCTCTCCAATAAAAGGACCTTCAATATGAATACCAATGTTTTTAGCACCTTGACATTTTGCTAAAGCAATATTTTTTAAAGCAATCTGAATATTTTCTCAACTTGCTGTCATTGCTGTAGGCATAAAACTAGTAATTCCATTTCTAGCTAAATTTAAACTAATATCTTCAACTGCTGCACTACTATCCATTACATCCTTATTCATAAAACCATGAATATGAGTGTCAATAAAGCCTGGAACTAATAAATATTGAGCCTTATTTTTAGTTGGAGTTATTTTAGTAATTTTTTTACTAAACTCAATGTCAGCACACTCAATAATTTGGTTGTGATTAACTATTTTAACGTTTTTTATTAGCATTTTTACCTCATTTGTTTTTTAATAATTTAATTATATTACTTAACTTTTTTTGCCTAAATTAAAAGCAAAAAATGGTAAATAAATTACACTTATTTGCTTTATTTTTGAAAAACCTTTTCAATAAGGTTTCATTTTCACAAATTTTTATTTTTTCCTCTATTTATATGTACATAAAGCATTTAATTGTTTATAATATAAACATATCTTTTAAATAATTGAGGAGTTATTATTTATGAAAAAAACTATTGACGATTTAGTATTAAAAGGCAAGAAAGTTTTAATGAGAGTTGACTTTAATGTGCCTCTTAAAGATGGTGTTATTACATCAAATAAGAGAATTGTAGCTGCTTTACCTACAATTGAAAAAGTTATCAAGGAAAAAGGAAAATTAATTTTATTTTCTCACTTAGGACGCGTTAAAAGTGAAGAAGATAAAGCTAAAAACAACTTATTACCTGTAGCTAATGAATTAGCCAGATTACTTAAAAAACCAGTACTTTTTGTTGACTCAACTAGAGGCGCTGAATTAGAAAAAGCAATTGCTAAAATGGAACCAGGCGAAGTGATTTTAGTTCAAAACACTCGTTATGAAGACTTAAACGAAAAAGCTGAAAGTAAAAACAATCCTATTTTAGGAAAATACTGAGCTGAATTAGGCGATGTATTTATTAATGATGCTTTTGGTACAGCTCACAGAGCACATGCTTCTAATGTTGGTATTGCATCAAACATTAAAGAAAGTGCTATTGGTTACTTAATGGAAAAAGAAGTTAATGCTTTAACTAAAGCTATCAAAAACCCTAAGAAACCTTATGTAGCTATTATTGGTGGAGCTAAAGTTAGTGATAAAATTCAAGTTTTAGAAAACTTAATTCCACTTGTAGACAAAATGATTATTGGTGGTGGAATGGCATATACATTCTTAAAAAGCCAAGGATACAAAATTGGTAAAAGCTTAGTAGAAGATGAATTTATTCCTTTTGCTACAGAATTCTTAAAGAAAAACAAAGATAAAATCATCTTACCGGTTGATCATGCATGTGCAAAAGAATTTGCCGATGTAGAACCAGTTGTTTATGGATTAGACATTGATGACAATTACGATGGACTTGACTTAGGACCTAAATCAATCGAAAAATTCGTATCTGCTTTAGAAGGCGCAAAAACAGTTGTTTGAAATGGACCTATGGGTGTTACAGAATTTGAAAACTACAAAACAGGTACTTTAGAAGTTGCTAAAGCTGTTGCTGCATTAAAAGGTTGCTACTCAATTGTTGGTGGTGGTGACTCAGTTGCTGCTGTTCAAAAACTTAAAATGGAAAGCAAATTCTCACATGTTTCAACAGGCGGTGGAGCAAGTCTTGAATTCTTACAAGGTATTGCACTTCCAGGTGTAGAAGCTATTCAAAATAAATAGTTTAAAAATAATAAAAAATGCGTAAAGCTCTGTTTTATAGGCTTAAACGCATTTTTACTTTTCTAAATATTCATTCATTTCAAGAGCTAACTTACGTGCATAATCACCAATAACTAATGAAATTTTATTAGTGCTAATCATAATTCCTGTAATGAATTTTGTATCTTGCAATTTATCCATTTTTATTAATGTTTTATCTTTGATTAAAACTTTAATATTTGAAATGCTTGCATTTACTTCTTCTATGTTTTCTTTGCCACCAAGGTTGTCTACTAATTCAACAACTTTGATATTTGAAGGTAGTTTAATATTAACTCCTTCTTTTTTAATCTTTTGTTCTTTTTTATTTTTTAGGTGTCAATATAATCAACAAAAACCTAAAGTTACAATGGTTAAAAGAACAACTAAAAATTTATCTTTTTTACTCATATTTTTATTATACAAAATAAAATTTAAAAGCAAATTAATAACTTTGAACTAAATTTAATTTATTACATTTCTCTTTATCTTTTACAAAGATAATAAAATTGTTGTAAAATAAAAAATAGTTATATATGCTTAAATAAAAAAAGAGGTAGTTATGAGCAAAAAAAGATTATTAAGTGGTATTAAACCAACAGGTGATTTAACCCTAGGAAATTACATTGGAGCTTTACGTAATTTTGTTAAATTACAAGAAAATTATGATGAAGCTTACTATTTTGTAGCTGATTTACATGCTTTAACAACAGGTTATGTTGATCCTAAAGAGTTATACCAAGCAAGAAGAGAAATAGTAACAATGTATTTAGCATGTGGCTTAGATCCTAAAAAAAGCACAATATTTTACCAAAGTGATGTTGTTGAACATACTTTGGCTCAATGACTTATGACTACTGAAATATCAATTGGTGAATTAAGCAGAATGACTCAATTTAAAGACAAAAGTCAAAAGATGTCAAAACAAGCTAATGGTACAGAAAAAATTCCAGTTGGCTTATTAATGTATCCAGTTTTAATGGCAGCAGATATTTTAATTTATAATCCTGACTTTGTTCCTATTGGTGAAGACCAAAAGCAACACTTAGAGTTAGCACGGACTATAGCAGAAAGATTGAACCGTGATTATAAAACGAACTTTAAGTTAGCAGAAGGAATTATTCCTCCAGTAGGCGCTAGAATTATGTCACTAACTGATCCAACTAAAAAAATGTCAAAAAGTGACAAAGGCACTAAAGCAACAATTTATCTTCATGATGATCCTGAAGTTGCTTACAAAAAAATTATGAAAAGCGTCACAGACAGTGAAAACAAAGTTTACATTTCGGAAGATAAACCTGGAGTATTAAACTTATTAAATATTTATGCTTCACTAACTAATAAAACTTTAAAAGAAGCAGAAAAAGAATTTAAAGACAAAGACTATGCTCAATTCAAGAGTGCTGTAGCAATAGTTGTCAAAGATGAATTAACTAAAATTCAAGCTAATTATGCAAAAGCAACCAAAATGGTTGATAAAGTTGTGGCCGAAGGCGCACAAAAAGCTCAAGCAATCTGTCAACCTATTGTTGAAGATTTGATGAAGAAAATGGGGTATAGATAAAATGGCAAAAGATAAAAAAATATTACAAAAGTGTTAAACCTGAAGAAATTTATATCTGTGGAATAGACACAGATTGTTGTGTTCAAGCAACAGCAGTAAACTTATTTGAAAAAAGGATTAGACCAATAGTTTTAAGCAATTATTGTGCTTCAAATGGCGGTCCTGAATCTCATGAAGCAGCTCTTAAAACATTATCAAGAGCAATAGGCAAAGAAAACATTATTCAACTATAAAACAGTTGTTTTAGAAAAAAGAAGGTATATATGAAAATACAAGTAGACAAGTTACTTAATCATACAACAAGTCACCTTTTAGGTGCCGCAGTTGAAAAACTTTATCCAGAAGTTAAATTAGGTTTTGGACCTGCAACCGAAGAAGGTTTTTACTATGATTTCGAATTTAAAGAACCTTTAAGTGTTAATGAATTAAGTAAGATTGAAAAGTTAATGAAAAAATTAGCAAGCAGGAATCTGGTAACTATTCAAATTCCTGAAAAAGATTATGACTTTAAAAACAAACCATATAAAAAAGAACTATATGATGAATTAAAAGCACAAGGCAAAAAGATTACTTTTTATGCTCTTCAAGATCCTCTTAATAAAGAAATAATATTTAAAGATTTATGTGCTGGTGGTCATGTTGAAAGTACAAAAGTTATTAAAAACTTTAAACTTTTAAGTATTGCTGGAGCTTACTGAAGAGGTAACAGTGACAATATCCAATTAACAAGAATTTATGGAACAAGTTGAAGCAAAAAAGAAGAATTGGATAACTATTTAGCAATATTAGCTGATAGAAAAGAAAGAGACCACAGAAAGCTTGGTAAAGAAATGAAAATTTTTGCTATGCATCCTTTATGCGGCCAAGGTTTTCCAATTTGACTTGAAGATGGAATGTACATTCATAATGAAATTAAAAACTTAGTATTAAAAATGGACCGTAAATATGGATTTACTGAAGTTTTAACTCCACACTTTGGTTCAGAAGACTTATACAAAACCAGTGGTCATTTAGCTCACTATAAAGATGATATGTTTGCACCATTAGTTATTGAAAACGAAAGATTGATTCCTCGTCCAATGACTTGTCCTCACCACATTGTGTGTTATAACTTAGAAAAAAGATCATATCGTGATTTACCAATTAGATATTCAGAACAAAGTCAACTTTATCGTTATGAAAAATCAGGTGCTTTAACAGGACTTGAACGTGTTAGAGGAATGCTTTTAACTGAAGGTCACTTATTTGTTAGAGAAGATCAAATTGAACAAGAAATTAAAAGTATGTATCAATTAATTGAAGAAACATTAAAAATTTTCAATATTGATATTAGCTATATTTCACTTTCACTTCGTGATAAAAATGACAAAGAAAAATACTTTAATGACAATAAAATGTGAAATAGCGCTGAAAAAATGCTTAAAAAAGCTCTTGATGATATGGGAGTTAAGTATACAACCGTTGTTGGTGAAGCTGCTTTTTATGGACCAAAAATTGATATTCAAATTGACACAGCACTAGGCCATGAAATTACTGTTTCAACCATACAATTAGACTTTTTACAACCTAAAAACTTTGATATTACTTATACAGATAAAAATGGTAAAGAAGCAAGACCGGTTATGATTCACCGTGGTTTAATCGGAACTTATGAAAGATTTGTTGCTATTTTACTTGAACAAACTAAAGGTAACTTACCATTCTGATTAGCGCCAAAACAAATTACAATTATCCCTGTTAATTTAAATGAAAATTTAGATTACTCTAGAAATATTTTCAATAGATTATGAAATTGTAATTTCAGAGTCAAGATTGATGATCGTGATGAAAGATTAAATAAAAAAATTCGTGAGGCTCAAATGTCAAAATCAAAATACCAAGTGATTTTGGGGGCTAATGAAGAAAAAGACAAAACAATTTCTTATCGTGAATATGGTAAAGAAGATACAAAAACTGTGACAGTTGAAGAATTTATCAACATTCTTACAAAATTAAGAAATAACTATGAATAGTAAACAAAAGAAAAAAGAAGTTAAAACATGAAGACCATTAGTCAATGTATTATTTACATTGCTCTTTTGTGTTGTTTTTCCTTTTGTTTGATGATTATTTGCAACTGTTGATTATCATAATCAAAAAATTACAAATTTAGCAATTTGTATTTCGGTAATTGTTATCTATTCACTTTTAATAATAGGATTAAATATTTTGCTTCATTATTTTAAAGTTTTAAATATTAGAAGTTATAACTTTAATATTCCAATTCTTTCAGTTTTCTTGTGAATTATTTTAACTTCATACATATCTAAATTTAATATTTATGCTCGTGTTGGAGTAGCTATTGCAGTAGTAGTTGTAATTACTTTAATAGTTAACTTTACAACAGGTAAAATTGAAGATAATATGGAGAAAAAAGCTAAACAATTAGAAGAAGAAAAACTTAAAAAAGAAATTGAGGATTTGAACAAATAATACTATAAAACAGTACTATAGGTAAATTCTAATAAAAAAATTATCAATAAATAAAACTAATTTATAATATTATTAATTAGTAGAGGAGTTTAGTAATGAAAAAAATAATCTGTGTTATTGACATGCTTGAAGGTTTTTGCAATGAAGGCGCTTTGGCAAGTCCAATAGTTAAAAAAATAATACCTAATATTGAAAAAGTATTAAAAGACAATAAAAAAGAAGACAATTTATTTATTTGCGATAGTCATAATACTTTTGATTTGGAGATGAAACAATATCCACTTCATTGCCTTAAAGACACAAAAGAGGCTGAAATTGTTAAGGAATTAAAACCTTATGTTAAAAGTGTTTTAGAAAAAAATTCAACTAATGCATTTCATCTTTTTGACAAAAAACTAATTGAAAAATATGATCAATTTGTTTTGACTGGTTGTTGCACAGATATTTGTGTTTTACAATTTGCTTTGAGTTTAAAAACATATTTAAATGAAAATAGAATTGATAAAAAAGTTATTGTTTTAAAAGATGCTGTTGCCACATTTGATGCTCCAGGACATAATGCTCAACAATTTCACAATTTCACCTTAAACTTAATGGCTAATGCAGGCATTGAAATTCAATAATTATGACTAAAACAATTGATTTACATAAATTAAATGTTGAACAAGCTACTTCAAAAGTTATTTTAGCTTTAAGTGAAGCCCAAGAGAAAAAACTTACTTCTTTAGAAATAATAACTGGTTATGGTAGTGGTGCTCTCAGAGCAAGAACTCTAGAATTACTAGAATCTGAAAATTTAACTTATATTGAAGAAGGACCAATGGTAATTGTTTATTTTTTTAATAATAATGAAGATGAAAACGATGATAATTTTTCAGATATAGAATATCAAAAGAAGTTCCAATAAAAGTGGGATTTCTTTTTATTTAATTAAAAAGCAAAATAAAAATAGCTTGTTAGGCTATTTACTATTTTTTGATTGTGTTTGATTATTTTCCTGATTATTATCTTTATTTTGTTCTTTTTCACCTTCAAAAGTTAAATCACTTTCTAAAGGCTTGTTGACAATTTCTTCTTTGACTGATATTTCAAGTCCAAAAGGAACTAATAATTGTAAAGCTAAGTATATTAAATAAAAGTCATCCATTAATTTATTTGTTAAGGTTTCAATAATATCTTCTTTATTAATGGTTTTGGCTTCAAAGATTAAATCAATTAATTCAAAAGGAATGTCAAATCAAATAAATGCTTCTGTATTTCTAATTAAGAATGATAAATTATTTAATAAATATTTTTTATTCGCTTTAGTGTTAGGAACACTAATTTGGCTAATAGTCAATTCATTGAAAATTTCATTTAAGAATTCTAAATTATTTTTTTGATCTAATCAAGCTTTTAAAACAGCATGATTATTTGATTTAAGTAAAAGTTTTTCGTAAGCTTTTGGTAATTCAACGCTTGTGTAATCACTTGGTGTATAGAAGTTATCTTTATCAAAGAATGACACATTTACTTCGGGATTTAGTTCTCTAAGTTTAACGGCAATTCCGAAGCGTTTAACACTTAGTTTTTGTTCGATTAATTGTCCATATGTTCTGCTTTTAGCTTTAATTAATTGTTTCTTAAGTTTATGATTAATTGAGGCTAAATCATGTCAAATGTTGCCTTCGTAGTAAACATTTTGCATCTTTAAATATTGGTTATCAGTTAAAGCTTCATATTGTTCATATTTTTTATGGATTGGAGCAAAACCAAATTCAAAATTTTTGAAATTATGTGTGTTTTTATAAAAATTGATTAAGTCATAATTTTTGTGAATTTTGTTATTTTCATCAACGATATTTTCTTTGTAACCAAGAGTAAAGAAGTTAAATAATTTTTTGATTAATTTATATTTATCAAATTTGGTAATAACATCTTTCAGCACATATTTTTTAGCTTTTTGTCCTATTAAAAGAACAATTAAGCCAGGGATAGTTAAAGTTAATGAAATACCAAATAGCATGCCAAAGAATCATTCACTATTGTTTTCGAAAAATGTTTCTTTTTTGCGAAAGTAAAGAGCAAAAAGGGCAATGGTTGTTATTAATAATAAAAGACCTAATAAAAATATTCCAACGCCACTTCATAAACCGCCTTTAGCTCAATTAATCCTTTTGCTTTTCTTTTTATCAGCATAATAATTATTAATTAATTGAGTAGTTAATTTGTTTATTGTTGGACGTTTAGAACGAATAAAAGCTTCTTTTGAAATTAACTTATTTGATACTGACATTTGACCTCATTTCTATACGTATTATTATAATATTAATTATTTAATTTATTTATTATTTTGTTGTTTTTAGTATAAAAAACTTTTGCCTAAGCAAAAGTCAAAAATGGTAATTTTATTAATGTATATTTAGTACCCTTCTTTTGAAGATTTACCTTCAACAATGGCAACTGAACGGCTTGTTCCGAAACGGTTTGCGCCAGCTTAAGCCATGTTGATCATGTCATCTTTGTTGCTGATTCCACCAGCAGCTTTGATTAATAATTTGTCTCCACAAACACTTTTCATGATTTTAACATCTTCAAGTGTTGCGCCTCTTGTTGAGAAACCTGTTGAAGTTTTAATGAATTCAGCATCAGAAGCCATAACGATTTCAGTAGCTTTTCTAATTTCAGCTTCTGTTAATAAAGCTGTTTCAATAATAACTTTTAAAACATGTTTGCCGCATGCTTTTTTAACCGCTTTAATGTCTTCTAAAACATAGTCATAGTCACCCATTTTCATTTTTCCAATTTGAATAACCATGTCAATTTCATCAGCACCAGCTTTAATAGCTTCTTTAGCTTCAAATGCCTTAGCACTTGTAAGCATAGCACCTAAAGGGAAACCAATAACTGATGTAATTCCAATGTTTGTTTTAGTTAATTTTTCTTTGCAGTATGCAACTCATGATGAATTAACACAAACAGTTTTGAAGTCATATTTCTTGGATTCATCTAATAAATTATCAATTTCTTTATTTGTCGCATCAGCTTTTAAATAAGTGTGATCAATCATTTTGTTGTAATTAATTTGCATAATTTTTTCTCCTAATTTTTTATTATTTCAACTTAGCAATTATAATCTTATTTTCAACTTTTGAATTGCCAATTTTGTAACCTGTTTCTAATTCTTTAACTAAATTCTTGTCAATCGGATTTGAAGAATAAAGAGTAAATAGTTTTTGCCCTTTAGTTACTTTATGATTTGTTTTAGCGTGAAGAGTAATTCCTGCTTCAAAGTCAATACTATCTTCTTTTTTAGCACGGCCGGCGCCAAGTTTCATAGCTATAATACCGAAAACTAATGAGTCAAAAATATCTAAGTAACCATCTTTAGCAGCAACAATATCTAAAGAATGTTTTGGACTTCAAAATTTAGGATCTTGTAATGCTTTAACATCACCACCTTGAAGTTTGACAAATTCATAGAATTTTTCAAGGTCTTTGCCATTTTTAATAACTTCTTCAACTTTTTTAAGCCCTTCAGCATGAGACTTAACTAATTTGGCTTGTTCTAAAATAGTTGCGCATGAAGAATAAACTAATTCATTGAAATCTTCTGGACCTTTGCCTTGAAGAGTTCAAATAGCTTCTAAAACTTCATTTTTGTTTCCAATTTCACAACCAATTGGACGGTTCATGTTGGTAATTTCTGCACGAACATCAACGTTTAATTCTTTACCAATTGAAATCATAGTTTTAGCTAATTCTTTAGCTTGGTCTTCATTTTTCATGAAGGCGCCTTTACCGCATTTAACATCTAATAAAATAGCATTTGAACCTGTAGCTAATTTTTTAGACATAATACTTGAAGCAATAAGAGGAATTGAGTTAACACAAGCAGTAACATCTCTTAAAGCATAAAGTTTTTTATCAGCTGGTACTAATTCTTTTGATTGAACAATAACTGAAATATTATGTTTTTTAACTTGTTCAATAAATTGACGTTCAGTTAATTCAACATGAAATCCTGGAATAGATTCAAGTTTATCAATTGTTCCACCAGTGTGGCCTAAACCTCTACCACTCATTTTCGCTACTGGAGCACCTAAAGCGGCACAAATAGGAGCAACTGCTAAAGTAGTTTTATCTCCAACTCCACCTGTTGAGTGTTTATCAACTTTAATCCCTGGAATTTCACTTAAATCAATAACTTCACCTGAGTGCATCATGGTTTTAGTAAAAGTTGCTAATTCTTTTTTTGCCATTCCTTGGAACATAACTGCCATTATAAAAGCAGCCATTTGGTAATCAGGTGTATTACCTGATACATAAGAAGTAATTAAGAATTCAATTTCTTGAGTTGTTAATTCTTTACCTTCTCTTTTCTTTTCGATTAAATCAACGACACGCATTATTTAACCTCCCTTATTTTGCAACACCTAAAGCAATTTCCATCATGTTTTTGAAAGCTGTTTGTCTTTCTTCGGGTGTTGTTTCGTCATTTGTAATTAAGTTGTCACTAACTGTTAAAAGACATGCTGCATTTTTACCTAATTTTTCAGCATTAGTAAATAAAGCATATGATTCCATTTCAACACAAAGCGCATGTGTTTCATCGATTCTTTGTTGAAGAGGAACTGATGAATAGAATACATCTGAAGAGTGTACACGGCCTAAAGTTAATTTAATGTTCAAATTTTTAGCCACTTTTTCAATTTCTTTATTTAATTTAGCACTAGGTAAAGCTACATGGCTTTTGTCTCCTAAAACTAAACGACGATAACTAGCACCATCTGCATAAGCTTCAGTTGCTAAAATCGTTTCATATAGTTTGATTTCTTTTTTGTATGAACCAGTTGTACCGATTCTAATAATGTTATCTACATTGTAGAATTTGAATAATTCATATGAATAGATCCCAATTGATGGACAACCCATTCCACTTCCAGCGATTGTTACTGGACGACCTTTATATGTACCTGTATACATGTACATGTTTCTGACAGTATTAACAAGTCTATATCCTTTGTCTAAAAATGTTTCAGCAATAAATTTAGCTCTTAAAGGATCTCCTGGCATTAAAACTGTCTTAGCAATATCACCTTCTTTGGCATTAATGTGTGGTGTCATAAAATCTCCTTAAATTAATGTTAAATAAGTACTTTCAAGTTTGGTTTATATTTAATTTCAAGTACATTATTGGACATCAACTAATAGAAATCTGATAAAGTGCGATTTAAGTAGTATTAGCGATTTAGCTTAATATTTTCTATATTAAACATCCGGTTATAATTTTAACATATATTAAATTTAAAATATATAAATATCACTATTAGAGCTATTTATTTGATTTTTTTAAGCTAAAGGTGTCATTTTTTCATATTTTAGTTGCCTAAAAAATACAAAAATAAAAATATTTTCATATTTGTTAATTATCAGGCTAAATAATATTTTTATTTAGAAACTACTTTTTATATAATTAGTTAGATATTTAGATACTTTTTTTGTCGAGGTGAATTATGAAAAAAATTAAGAGAATTGCTATTCTAACTTCAGGTGGCGATGCTCCTGGAATGAATAGTGCAATTAGAGCAGCAGCTAAAATGGCTCGTAGTCGTGGAATTGAAACATTTTTAGTATATGAAGGTTACAAAGGATTAGTTGATGATAATATTGTGCCTTCAACAAAAATTGATTTAGATTTCTTTAACAGTCGTGGAGGCACTTGTATTTATTCAGCAAGGTATCCTCAATTTAAAGATCCAGAAATTCGTGAAATTGCTATTAAAAATTTAAACAAACGTAAAATTGATGCTTTGATAGTTATTGGTGGTGATGGTTCTTATGCAGGAGCACAATTATTACATGAAAAAGGAATTAAAACTATTGGACTTCCAGGGACAATTGATAATGATATAGCTTCAAGTGATTTTACTATTGGCTATGATACAGCTTTAAATACAGTTGTTAGATGTATTGACCAAATTAGAGATACAGCAGCAAGTCATAAAAGAATTATGATTATTGAAGTTATGGGTAATAACTGTGGTGACTTAGCATTACACACAGGACTTGCAACAGGGGCAGAAGTTATTGCAACAAGTGAATACCGTCTTGATGAAAAAGAAATAGTTAAAACTTGTGTTGAATTAACTACAAATGCTAAACCTGTTAGAAGAAATATTACAATAGTTGTTAGTGAAAAATTATATAACATTAAACAACTAGCTAAAGACATTGAAAAAGCAACAGGATGAGAAACTAGACCTAATGTTTTAGGACACATTCAACGTGGTGGTAATCCTTCAGCTCAAGAAAGAATTCTAGCTTCACTTTTTGGAATTAAAGCTGTTGAATGTTTACTTGAAGGTAAAAGCGGTGTGGCTATTGGAATTATTGATAATGATATTGTTGCTTCACCAATCTCAGAAGCATTAAGTATGATTAATAAATCTAAAGCTAAAGTTAGAAATAAAGCAATTAAATTTAATGAATTAAACCGTTTAGATTAATCAATTATAATGTCATTTATTTGGCATTATTTTAATTTTTTAAGCAAATTTTTCATTATTTTTTTTAGAAAAATATTTAAGCTTATTTTTTGAATAATAAACCTATAAAACAATGTTTTTGGTAAATTACACTATTTGCTTTAAACTAAGATTTTTAAAAAAATGTTTTTGTTTTTTTAATAATAAATTATTATTATATAGGCGCATTTAGAGAAAGGGGCAAATTATGAGTCAAAAAGAAGATAAAAAACCAATAGTTTTTAGTGATGTTGATGGTACACTTTATGCTCGTGATTGTTATGTATCAAACTTCAATTTAAACATCATTAAACAAGATAATTTAAGTTTTAATATTGCTACTGGTAATCCAATTTGTCCAAGAATGTTAAAACTTGCAAAATTAGTAGATGCTGATTATATGATTGGATCTTCAGGTGTACAAATTTATGATTACAAACATAATAAATATTTACATGAAGAATATCTTAAAGCAAGCGTAGTTAAAAAGATTTTTAAACTCTTTAAAGAAAGCAAAGTTTCAGCTGCTGCTTGAAGTAGTGATGTTTTTTATGTATTTAAAGAAGCAGATAAAGAATTCCTAAATAAAATTTATTATCGTTATGAAACTTTTGAACAATTCGAATTATATGAAGATCAAAAAGACATTAAAAAAGTAGCCAAAATTGAAGTTTATTTTGAAAACACTCCAAATGTTGGCGATTTAATTAAAGAACTTGAAAAACTTGATTGCCAAGTAATTGTGACACATATGAATCTTGAAATTATTTCTAAAGGAGCTTCAAAAGGAAATGCTATTCTTTGACTTCTAAAACATGTATTAAAAGGATATAACCCAGAAGAAGTTATGGTTATTGGTGACAGTGAAAATGATTATTCAATGTTCCACAAATTCAACTATTCTTATGTTATGGATAATGCTAAAGATGAAGTTAAAGATAAAGCTAACTTTATAACCAAAGCCGTTCAAGAACATGGTTTAGGTTATGCTGTTATGGATTATTTAGAAAAATTTAACAAGAGAAATAACTAGTCAGTGTACTAGTTTTTTTAATATTTTGAATATTTTTAATAAAAAATAAGCAAATTTGTCAAAAAACCTGCAATATTACATACTTTTTTTACAAAATTAATCAAAAATTAATCAAAATTGTTAAAAAAGTCTGTCATTATGACAAATTAATTGTATAGTTTCTTATATAAATATAAGGAGTTGACTATGACTATTGAAAGAGATAAATATTTAAATGGTCTTTTAGAATGAAAAGATAAAGACATTGTAAAAATAGTAACTGGTCCTCGAAGAAGTGGCAAAACTTTTTTATTATTTAATCTTTTTGCTTCTGAATTGTTAAAACTTGGAGTATTGAGTCAAAATATTATTCAAATAAATTTAGAACTTGAGGAAAATAAAAAACTAAGAAAAAGTCTTAATCTTTTAAACTTCATTAAAGAACAAATAATAAATGAAAACAAATATTATGTTTTAATCGATGAAATTCAAAATGTTAAAAATTTTGAAGATTCAGTTAATTATTTATTAAATATTAAAAATGTTGATCTTTATATTACTGGCAGTAATTCTAAATTTTTATCAAGAGATATTGAAACTCAAATTAGTAGTCGAGCTACAGAAATCAAAGTTAGACCATTAACATTCAAAGAATTTTACAGTGTTAAAAAAGAATTAAACATCGATGAAGTTTTTAAAGAATATTTGACTTTTGGTGGTATGCCTTTTTTAGTTAATTTAAATTATGAAAGCAAAAAGAAATATTTAAAAGAACTTTTTGAAACTACTTATATTAATGATATAGTTGAACGCAATAAAATAAAAAGTGATGAATACTTACAAGAATTTGTTGATTTTTTAACTTCTAATGTTGGTTCATTAACTAACAATTTAAATATCTGTAATACATATAAAACACTAAAACAAAACAATATAGCCTACTTTACTATTGATAAATATCTTAAAAATTTATCTCATACATATTTGATTGATTTAGTACAACTATTTAATTTAAAAGGAAAAAGAATAATCAAAAGATTAAACAAAATTTATTTTTATGACTTAGGAATTAGAAATTCAAGAATCGGCTACAACAATATTAAAATCGAACATGCAATTGAAAACATTGTTTACAATGAACTAGTTATAAGAAACTATGATGTAAATATTGGTTAAATTAAAGTTAGAGAAAAAAATAAAGATAATCAATTAATCACCAAAAACTTAGAAATAGATTTTGCGGCTTCGAAATATGGTGAAGAACTTTATGTTCAAGTAGTTGATAATTTAGAAAATTTTGACATTTTAAAAAGAGAAATTAAACCTTTTTTAAAATTAAAAAATAATTATCCAAAATTTATTTTATATCGCACTAAAAATATTGACATTGACAATGTAGATGGTATCAAATAAAAAGTATTTGTGATTTCTTATTAGAAAAATAATTATTTATATATAATTAAAAAACTTAATTTAATATAGGAGGAACTATGGCAAGATTTATTGATGAAATCAAAATAACTCTTCAAGCTGGTAAAGGTGGAGATGGCATGATCTCTTTCCGTCGTGAAGCTCATGTTGACAAAGGCGGACCAGATGGAGGAGATGGTGGAAGAGGCGGAAACATCTATTTTATAGGTGATTTAGGAAAAAACACTTTACTTAATTTATATGGAAATAAAAAGATAACTGCTGAAGATGGTATTAATGGCGGGCCTAAAAATCTTTATGGTGCAGCTGGCAAAGACACCTATGTTAAAGTTCCTCTTGGTACTGTTGTTTATAAAAATAATAAAGTGGTAGCTGATATTATTGAGCCTAATAAAAATTATTTAGTAGCTCAAGGTGGCCAAGGTGGCCGTGGCAATATGAAATTTAAAAGCCCTAGAAATACAGCTCCTAGAATTTGCGAAAATGGTACAAAAGGCGAAAAATTTGAAGCACATATTGTACTTAAAGTTATGTCGGATGTTGGTGTAGTTGGTAAACCTAGTGCAGGTAAAAGTACACTACTTTCAGTAATTAGTAACGCTAAAGCAAAAATTGCTGAATATGAATTTACAACCTTAGTTCCTCAATTGGGATTGGTTAGATATTTTGATAATTCATTTACTGTAGCTGACTTACCAGGATTAATTAAAGGTGCTAGTTTAGGTAAAGGTTTGGGTTTTCAATTTTTAAAACATATTGAAAGATGTCGTGTGATAGCTCATATTATTGACTTTGGTTTAAGTGAAAAAAATCCAATTGAAGACTATGAAACTATTAATAATGAATTAGCTTCATATAGCATGCATTTAGAAAATAAACCACAAATTGTGGTCGCTAATAAAAGTGATATGCCAGATTTTAAAGATCATCTTAAAGCGTTTAAAAAGAAATATCCTAAAGTTAAAGTTATAGTAATTAGTGCTTTAGAGCAAGAAAACCTTGATCAATTAAAAGCTGAGCTTTGAAAAGTTATTGAAGCAAATAAAACTACTCCAGTAGCTGAAGTTGAAGAAGTTGAAATGGTTGAAATTAAACTTGAAGATGATTTCACTATTGCAAATCCATATCGTGGTTTCTTTGAAGTTAGTGGGCCAAAAGTTGAAAAAATCTATGAAAAAATCCCTCTAATTTCTTATGATAATTTGATTAGATTTAATAATATGCTCAAAAAAATTGGTGTATGAGATGATTTAATTAAAAAAGGAATTGAACCAGGTGATACTGTTCGAATTTTAGATTATGAATTCGAATGAGATGGCGAATTTTAGATAAATTTTTAATTAAGTTTAATTGATTGATAGTATTAAAAATTAAAAACATTTTAAATGTAATATAATTTTATAGAATTATTTTAAGGAGTCCTATGGCAATCAAATATTTAAGTATGAATAATTATTCTGATATTTGTGAATATGTTTCACAAGTTATTGAAAATCAGTTGTTTATGAAACCTAATTCAAATATAGCGATTAATGCAACTGATGATTTTAGAGGAATTTATAGTCAAATTGTTAATGATGTTAAAAAAGGAAAATACAGTTTTAATAAAGTTAATTTCTTTTTAACTGAAGAAATTGCAAATGTAACAAAAAATACAATTACTTCTGTTTATAGTTTCTTTGATAAGAATCTTTTAGCGCCAATTAACATGCCAACTAATCAAATTTTCTTTCCTATAACTTTCGCTCCAACAAAAGATCAACAATATGATCCCTTTATTTTCAAAAATAAAGCATTCGACTTGTCTATAGTGTTTTTGGGCAAAAACGGTGAGCTAGGTTATATCGATAAATTTAAGAATTACAACTCAGATTACACATCAAATTTTTGATTAAATAACGATTTATTAAACAACTTTAAAATCATGCAGAGAAATAATCAACTTAATTCACTTGAAATTTATTCAATTGGTTCTGGTGCATTATTTAACTCGAAAAAGATTTTAGTTATTGCTTTAGGACTTGATAAAAGCAACACAATTTTTAAATTAATGAATACTAAAACAGTAAACTCTAAATTTGCAGCTTCAATGTTAATACAACATAATGATGCAACTTTAATTGCTGATGCTCCTTCGGGATTAGCTATTAAGAAAAACTAAATTTGATTTAAAAATGACAGCATTACACTGTCATTATTTTTTTAAATATTTTTTATCAAAAATTCCAATATAAGGGAGGTTTCTCATTTTTTCATCATAATCCAAACCAAAACCAACTAAGAATTCATTTGGCACGATAAAGGCTGATTTATCAGCTTTCAATTCAACTTTACGGTTGTGTGGTTTGTCTAAAAGAGTTATTATTTTAAATGAACGAGGTTTACGAGATAAAAGAATTTGCTTGATTTTATCAAGTGTAATTCCTGAATCAATAATGTCTTCAACAATTAAAACATCTTTATTTCTAATATCTTGAGCCAAGTCCATAATGATTTTAACACTACCGCTACTATTGCCTGCTCCTGCATAACTAGAAGTAGTCATAAAGTCTAAAACATGGTCAACAGTTATATCTTTTATTAGCTGAGCTAAAAATGGGATTGATCCTTTTAAAAGACCAACAATGATTAAATTGTCTGAGTCTTTGTATTGTTGATTAACTCAAGTTGCTAATTCTTTGATTTTAGCTTCAATTTCTTCTTGAGTATATAAAACCTTTTTAATTCGATAGTCAATATTTTGCTTATTCATTATACCTCTTTGATTTTGAGAAGTCATAGCTATCTATTATATTAATAGCTTCTTGTAAAACTTCTTTTTCACTTTTGTTATTACTATTAATGATAACATAAGGTATCTTAAATTTTTCAACTAATCTTATATAAAGCTTTTTATATAAAGAATGAAGTAATTTAAAGTATGTTTCATTTTGAGCATAATTATCAACTTCTGAAGCGCGACCTCGAGCTAATAAACGAGCTTTGAATGCATTAAAATCAATATCAATAAAGATCGCCAAATCCGGATTATCTTCAGGATCAACTATTTTTTCAAACATAGCATCAAAACCTGCTTGATATTTAGGATCTTTATCCATTAAAGTGACAACAGCAAAAACATAGTGTTCGATGTTGAAACGATCTAAAAAGATATGATTGTCTCTAAAATTTAAGTTATTATTTTTAAATTTAGCTACTGTTTTTTTAAATGTGTCTGATAAGCTTTCAATAATATATGATTGAAAACCAATGTGAATATTAGGTTGTTTTTCATACATTCATTTTAAAAATGTATTAAAAACTGGGTCATTTTCTTGAAATTCTTCTAATAAAACTGATTTTTTATAATGTTTATGTAAGCCTTTAGCAAGTGTACTTTTACCACTGCCAATCATGCCACTTATACCAATAATCATTGTAAGTCCTATTTATTGTAACGTTCCATTTTACTGAAATCGTAATTGTTAATAACTTCAACAGCTTTTTCAAAAACTTGTTTTTCAGTTAAATTATCTGTTTCAATAATAACATAGTTTAAATCATATTTTTTAGCTTGTTTAATAAATAATGGTTTATAAAGATCATAAAGTTCTCTAAAGTAATCTTTGTTTTTATTTCAGTTTTCAGTTTCAACTTCTCTACCTCTTTCAAATAATCTTTTCTTGAAAGTGTCAAAAGTCATATCTAGAAAAATTGCTAAATCTGGAGTTTCATCTTTAGTAATAAGATGAGAGAAAAGTGCATCATAACCTTCTAAATATTTTTTACCTTTAGTTCTTAAGTTAACATTAGCAAAAATGTAATGTTCAATACTAAAACGATCTAAAAATATGTGATTATCCTTGTATTTATAGCCTTTTTCGCTAAATTTTTTAAAACAATCTGCTAATTTAGATGTGTGGTTTTCCACTACATAAGATTGAAAACCAATTGTTAAATTAGGTTGTTTTTCATAAAGTCATTTTAAGAATGTATTGAAGACATCATCGTCCTCTTCATATTCTTTTAACATCATTGAATTTTTGTAGTGTTTAACTAAATTTCTTGTTAGTGTGCTTTTACCACTACTAATCATTCCGCTTATACCTATAACCATAATAATTACCTCTTTCTTTATTTGTTTTTAGTCTAAAAAGTTAATAAAAAAACAATACAAAACTGATTCATGTATTAATCATTTAAATGAAATCAATTGAATAGTCAACTATTTGTATTGAATTGATAAGATTTTTATACTGTATTTATTAGGCACATCAACTTCAACTGTTGCGCCTTCATCACCTTCCATGATAGCTTGTGCTACTGGTGATTCATTAGAAATATTACCTTCCATTGGATTAGAATCATGAATACCCATGATTTTAACTGTAATTTCTTTATTTGTTTTTAAGTTAAGATATTTAACTGTTGCTCCGATACCTGCTTTTAGTTTACCTTTTTTGTTTTGGTTTGTTTCAATAACTTCAGCATTTGCTAAAATGCCTTCAAGTTCAACAATTCTTGCTTCAACAATACCTTGACGATCTCTGGCTGCATCATATTCAGCGTTTTCTGAAAGGTCACCTTGTGCTCTAGCTTCTTTTAAAGCTGCTTGAATTTCAGGTCTTTCAACTTTAATTAAGTGTTCATATTCTGCTTGATATTTTTCTAATGTTTCTTTAGCTAATATGATTTTTTCTTGCTTTTTACCTGCCATCTATCCCTCCTTATATGTTTAATAATAGTTATTTTACAATATATTAAGCTTTATTTTATAAAATAAATTTATTTTTTGTCCATTATTTGAGAAAAGCTAAAAAACGATAAATAATCGATAAAAAATCAACTTTTTAGTGATCTAAATTTAATTAAATATTTAAGTGGAATTTTTTCTTCTTTATATAGAAAAAAGATTTTTAATATAGAATATTAATTATGCATAAGAAAGGAATGAATATGAAAGTTGAATACTTAAGTGAAGCAAAAGTCGAAGAATATAAATGACTTAAAAATTTTTCGAGTAAAGTTCTTATTTTTTGAACAAAATAAGATATTTCAGGAATTAAGGGTTTTAACTCCCATTCGAAAAACTTCAAATTACATCATCCAATTTCAAAATGCCTGAAGTATGTTCGCGAATTTTTTTGATTATTGTTGTTGCTTTCATAATGAATAAATTAATTATTTAGTTTAGTTTTTGTTAAAAAAAGAACATTTTCGTTACAAAAAAGTCCTTAGTTTTCATTTTTTATTTCAGTTTTACTTTTTACTATATATAAGAATCCGCTTTTTACATAACTTAATTCATAAACAATTTTATTAAGTTTTAAATGTTTAGTTAAAACTCTATAACCACTTTTTGTATTTGAATTTTGTAATACAACAAGCATCCCTTTTTCACTTAACATTGGATAATATTTGTCAAATAATTCTTGATTTGTTTCAGTTGAATTAATTGAAAAAATCAATTTGAATTTCTTATCTTGAGTTTTTAATTCATTAACATTAATAAATGAAGGTTGATCTTTAAAGTATTTAGGTAATTCTAAAACAGCTTTATTTCATAAACTTACATTCATGTTTTGTTTTTCTAAACAAATTTGAGCTTGTGATTTATTTTTAAATAAAGCTGTTGGATATTCTAACTTACTTCCAACAAGTAAAATCTCTTGAACATCATTTAAATAAATAGTATTAATAAAGTTTTCTAAATCATAATCATCAAGATTACTTTTAAAAAATTCTTTTAATTCTTTAGGGAATTCTCCTAATTCTTCACCTCTGATTTTCAAAATAGCTTCATGAGCTTTTTGAGCTTCTTTGGTATTTATTTCATTACGAATTTTTCTAGTTTTAACTAGTAAAATAATATAAGTTATAAAGGCAATAATCACAAAAACAATAGCAGAAACAATTAAGCCAATTTTTGCACCTTTACTTAAATTGATTACAGCACTAATCATGTAATTCTACACCTCCATAATTTAAAAATTCTTGCAAGATTAAAACAGCTGACAAAGTATCTTTATGAGCTTTTCTTTTTTTAATAGAAAGTTTTGCTTCTTTTAAAATATTAGTAGCAGCTATAGTAGTTCCATATTCATTAACTTGATAAATTTTGTAGTCTTTAAAAATACCTTTTAGTTTATGAGCAAAAGATTCAAAAAGCACTGTTCTTTCACTACTTGTATTATTAGATCTTAAAGGCTTTCCTAATACCAATTTATCTATTTTATATGTTTTTTGGTAAATTTTGATTTGCTCAATAATTGCATTAAAATCATTTTCTTCAAAATGTAATGTTTCAAGTGCACTAGCTATAATCATGCCTTCATCACTAATTGCAAAACCGCAAGTTTTAGTGCCTAAATCTAGAGCTAAAGCTCGCATTAAATAAGTTCCTTAATAATATTTTCAAGATCTTTAGTATAGTCAACTTTACCCATTGAAATAATTGAACTTCCACCACCACGACCATTGGTTTTTGAAGCTATTTGTTGGAATAATTTGTTAGCATCAAGCACTTTAGCGGCCACACATAAAAGAATTTGATTATTACTTTTAGCACCTAAAACAATTATAGCTTCAGGAAATTTTTCTCTAAGCGTTGCAGCAGCAACTTTAACTTGGGAACTATCAAAATCAATGTTTAAATAATAAGGGTATTTTTTGTTTTTAGTTAATTGAACACTATCAAAATCAAAGGTTATATTGCTTTTGTTTTTGATTAATTCTTTGATATCTTCATGAACTTTATCAATTGTTTTTTTAATGTTTGCACATTCAATTTCAACATCTTTATTAGGTGTAAATTTAATTTGATATTTACTATCAAAGCTTTGAGCTTTTTTAACTAAAGCATCTAATTGTTCTTTTAAACTTTGATTTAATTCTGTTAAATATTGTTTAACATATTTATGAGAAGCTACAGCTCTTATTCTAAATACACCAGCAGTTTTTTTCTCAACTTTAGTAATTTTAAAGTCTTCTAAAATTCCGGTTCTATCTAAGTGAGTACCACCGCAAAGGTCTTTTGTAATACCTTTAAAGTCAACAATACGAATGTTATTAGGATCCATGTATTCACTTTCGTCAATAGTCATAATTGCGCCCATTTCTTTGGCTTTTTTAATGTTTGTTACAATATATTCACGTTCAACATTTTGTTTAATTCAACCATGCATTATGTCTTCGATTTTTTTAATTTGTTCATCAGTTGGTTTTTCATCAGCTGGAAAGTCAAATATTAAACGTTCTTCGGTGATATCTGAACCAAGTTGATCAATATGAGGTCCAAGTACTTTAACTAAAGCACGGAATAATAAGTGAGTTGCTGAGTGACCTCGAGCCATACCAATTCTTACTTCTTTATCAACATAACATTCGACAGTATCCTTATTGTTAATTTTGCCTTCAATTTTATGAATATGGTTACCAAATTTGTCTTTAAAAACATCTAAAATTTTGATTTTATTTTTACCTTGTAAAATGAAACCATGGTCATGATTTTGACCACCACTAGTAGCATAAAAAGGTGTTTTATCTAAGATCAAATAACCTTCACCATTTAATGATTTTACCTCTTCCTCATTATTTAAAAGTTTAAGAATTTTTGATTTACTTTCTAAGTCTGTATAACCAATAAATTCACCGATTTTGCCTTTAATTAAGGCTAATGAGTTAATTACTTTATCCATTCCGTTAACTTTTTCACCCCTGCTTAAGTTGGCGTGTCTTTCCTTAGCTTCTTCAAAAGCTTTAAGATCAACTTTAATATTTTTCTTAGCTAAAATTTCAACAGTTAACTCAATTGGAAAGCCATAAGTTTCAAGTAAATTAAAAGCATCTTCACCTGAGAAAATTTTAGTTTTATTATCCATAAATTTTTCAAGTAATAATTTACCTTTTTCAATAGTTTGATGGAAAGCTAATTCTTCTTCTTTAATGGCTACTACAACAGGTTTTTTGTCATATTCATAAGGTAATGAATCTTGTACTACATCTACTAATTTATGTAAAAATAGACCTTTAACTCCTAGTTGCATTCCTTTGTAAATTGAACGTCGAATAAGTCTTCTAATAATGTAACCACGGCCAACATTTGAAACTTTAGCTCCATCAGCTATAGCATTAACCACTGTTCTAATGTGGTCTGCTATAACTTTAAAGTTAGTGTTGATTTCAGTTTGCTTTTTATCTTTTTTAAAGTAGTTATCAATGTCATATTTAACTGTGGCATATTTTTCTATTTCTTTAATTATGTTAATAAAAAGATCGCTGTCATAGTTAGTTGGAGCATCTTGCATAATTGAAGCAATTCTTTCAAGTCCTGCACCAGTATCAATATTTTTTTGTTTTAATTCAGTATAGTTATTTTCACCATCATTATTGAATTGACTAAAAACTATATTTCAAATTTCGATGTAACGGTCATTTTCAATATCTTTTTTAAGCAACTCTAAACCACGTTTGTTATATTTAGGTCCTCTATCATAAAAGATTTCTGTATCGGGTCCACAAGGTCCACTACCAACATCTCAGAAGTTTGTATCTCTAGTTCCTGGAATTAAGTGTGATTCTTCAACTCCTTGTTCCATTCAGTATTTTTTAGTATCCAAATCTTCTGCAAAATAAGTCATGTAAAGTTTTTTAGGGTCTATTTGTAATTCATTAGTTAAGAATTCATAACCAAAAGCGATAGCTTCTTTTTTAAAGTAATCGCCAATTGAAAAGTTACCTAACATTTCGAAAAATGTGTGGTGTCTTGAAGTTACACCAACGTTTTCTATATCATTAGTTCTAATTGCTTTTTGACTGTTAGTTAATCTATTTCTAGGCGGTTTTTTCTTACCTGAAAAATAATCTTTAAGTGTTGCTACACCTGAGTTAATTCAAAGCAAAGAAGGATCATTAACTGGAATTAATGATTTGCTTTCAACACGCAAGTGACCTTTTGATTCAAAATACCTTAGTCACTTTTCTCTAATTTCTTTTGATGTTAATTTTTCCATAGTTATCTCCTAAATGTAATCTTTTTTAATATTGTTTAAGTATATTTCTTCAATTATTGCTCCGCCTAAGCACTTGTCTTGATCATATAAAACAACTTGTTGACCGGGGGTTACTGCTTGAGCTTTTGATGGATAAAATACTTTAATTTTATTATTTTCTAAGAATTCAACTTTAACTTTAAGATCTTTTTGACGATATCTAAATTTAGCACTTAATTTGTTTTTATTAAAGCGATGATGATTTAGACATAAGTTTGAAGCTATTAGCATATTACTTTCTAAATAACTAGGATCACTAGCTGGTGCAGCATAAATAATATTGTTTTTGACATCATGACCGCAAACATAGTGAGCTTCACTTTTGCCGCCTAAATTTAATCCTTTTCTTTGACCGATAGTGTAATAGAAACAACCATCATGAGTTCCTAAAACTTCTTTAGTTTTAATGTCTAATATTTTTCCTTTTTGAGCTGGAATATAGTTTTGTAAGAATTTTGTAAAATGTCTTTCGCCAATAAAACAAATACCTGTTGAATCTTTTTTTGTAGCTGTAGCTAAGTCTAATTTTTTAGCTATTTTTCTTATTTCTTCTTTAGGATAATCAGCAAGAGGCATTAAAACTTTTTCTAGTTGTTGATTAGTTAATTGAGCTAAAAAATATGTTTGATCTTTATTTTGATCTTTCGCCCGATAAAGTTTACCATTCTTAACTTTGGCATAATGACCCATGGCAATATAATCAGCATTGAGTTTTTCAAAAGCATATTTTAAGAAAGCATCAAATTTAATATATTTATTGCATAAAATATCAGGATTAGGTGTTCGACCTTTTTTATATTCTTCAATAAAGTTTTCAAAAACATTATCTCAGTATTCTTTGACAAAATCAACACGATGAAGCTTAATACCTAATTTATTTGCTACTGCTAAAGCATCTTGATAATCTTGTTCTTGAGGGCAAATATCTTGACTAATATTTTCATTGCCTAAAAAATCATTATTAACATATGAATCTCAATTCCGCATAAAAAGACCTTCAACATCATAACCTTGTTGAAGTAATAAATATGCAGCTACTGAAGAGTCTACTCCTCCAGACATTCCTAAAATAACTTTCTTTTTATGCATATTAATCCTTATATTAAATATTAGTTTTAATTATATTATTAATTTTTATATTAATAATCATTTTTTAAGAAATGTCAAATAAAACCACACAAGTGTGTGTCTTATAATTATCCTTCAATTCACCTAGGTGGGTAATTTGTTTAGTTTTTAGCATTTTATTTTATTTCTATAATTTTATTGTGAATTTCATTTTCGATAAAGCTTTTCCGCTATTTTCTATAGTAAATACTTGAGCAAACTCATCATAGCTATCTTTAACATTTTATTTTAAAATATCTTTTAATGAAGCCATTTTTACTCTGTTTCTATTAACATCTTCATCAGTTAATTTTTGTATTAATTTTTTTAAAATAAATCTTTTAAATGTTATTAGAATTTTATATCTAATAAAATTACTAAATAGACTGTTTTATAGTTAAATTGGACAATTTTGAGTATATTTTTCAAATCAATATTAATCAAAAAATAAAACAATCAAAATTAAACATTCAAACAGTTGATATATAAGTTTAAGTTAATTAATATTGCCTATTTTTAATTTAGTTTTTTATTGTCCCTTTCTATTTTTAATCAAAATATAATTATTATTTTTTCTGAATCTATTAAAAAAACATAATTTTTTGACTCTTTAATAGCGAATTAGAATTATAAAAAAGAAGCACTATTGAGTACTTCCTAAAATTATTTTGAATTACATTTTGTGTTTTGAACTAACACCTAAAAGTCTTAATCCGTTTTCTAAAACAATATTAACAGCACGAGCAATAGTTAAAGAATTCATTTCATTCTTGCTTCCAATAATTTTTTCTGTATTTGAGTATCATGAGTTAAATTCTTTGGCAACTCTAATTAAGTATTGTGTTAGTAAGTGTGTTAAGTACTTAGTTGCTGAACTAATGATAACTTCTGGAAATTCAAGCAATGTCGAAACTAATTTTTTATCAAGTTCGTTGTCAATATTTGTTGCTTTAAGTTTTGAAACATCTAATTTAGATTTCTTAAGCAATTGGTATGAACGAGCATTTGCATATTGAATTAAGAAGACAGGGTTGTCATTTGATTTTGAAGTTGCAATGTTAATGTCGAAGTCAAGTTTTGAATTATATGTTCTATCTAAAAGAATGAAACGAGCACTGTCTACTCCAACAAGGTCAACAAAGTCTCTTAATCAAAAACTTGTACCTTTACGTTTTGACATTTTGTATTCTTGACCATCTTTCATAAGTCTTACTAATTGTAAACAAAGAACAATTAATTTGTTTGGGTCGTAACCTAAGTCTTGCATTGCTATTTTCATTCTAGTGATGTAACTTAAGTGGTCAGCGCCTCAAATGTTCATAATAATTGGATCTTTGCCTGAGTTTTTGAATTTTTTGTCATGGTAAGCAATATCTGGTGCAAAGTAAGTAAAATCACCATTGCTTTTAATTACAACACGGTCTTTATCATCACCATATTTTGTTGTTCTAAGTCATGTAGCACCATCTGCTTCAAAATGGTTTTTAAGTTTTTTAATTGTGTCTAAAATTCTTTTACTGTTGTTTGCATATAAAGTTTTTTCACTAAATCAAATATCAAATCCACATTTAAATTTTTTAAGATCAACTTTAATTTGTTTTAAGAAATTAGCCACACCTTCTTTTTTAAATACTTTTAAAGCTTCATCATTTAATTCTTTAAGATCTTTAAATTTATCTCCATGTTTTGTTCTAATTTGTTCAGCATTTCAAACAATGTCTTGACCTCCATAGCAGTCTTCTGGCATAGGGAAATCTGGTTCATAAAATTGTTTATATCTTGCAAATATACTTTGAGCTAAAGTGTTAATTTGGTTACCACCATCATTGATGTAATATTCACGTAAAACATTTAAACCTGCGAAAGTACAAATGTTGTATAGTGCTGATCCAATAGCAGCATTACGTGCATGACCAATGTGCAAGTATCCAGTAGGGTTTGCTGAAACCCATTCGATGTTTAGATTTCCTTTTAATTTACCACGACCAAAGTTTTCATTTTGTTTGATAATTTGGTTCAAACTTTTAATAATTGTTTGATCATCAATGTAAAAGTTTAAAAATCCAGGAGCTGCAATTTCTACACTTTTTAGACCTAATGAAGTTAGTTTAGGCTCTAATTCTTTAACAATTTTTTTGGCAAGTTCCATAGGATTTTTGCCTTTGTTTCCTAATGCAACGTTAGAAGCGAAATCGGCTTTGACTTTAGGTTCAATTAAGAGATCGTCCTTTTCAATCCCTAATTTGTTTAAAATTGGTTTTAATTCTTTAATAATTTTGCTTTTAATAATGTTTTTATCCATAATGTCAATATTATAACAAATTAATAGTTAAAATATATGCCTATTTTAATATTTTATTTTTTTGTAAAAATAAGTATTTTTTAAGGTTGTTTTTGCTAATTAAGCTTTTTTATAGGTTAATTGAAAAAACAAAAAACTTCACACAATAAATAATGGAGATTTTTATATGTGCAAAGTTTTTCTTTTAATTTAATTGAATGAAAAGTTTTTTTGATGTTACTTATTAAAAGTAATCTTTCAATATTGCATCTTTATATGATTCAACTTCATTTCATAAGTCATTTAAAATAGTATTTATTTCTATTATTTTTTTGTCTTTAATTAATTCGCTGTAGTAATTTTCAGATTTCATTTTTTCGCCAAGTTTTTTAGCTTTATCTCTTTTAGCAATAATTGCAGAATCAGTTATTTTTGTAGTTTTTTGATACTCTTCATAAGAGTCATTTATTTTGGCTAAATATGCTTTAATGTTTTCAAATTTAACTTTAGCAGCTTCTAAGTATTTTTCTTCATCAGTTTTACCATCTCCACAAGAGACACAAACAACTGATACAGGTAAAAATGAAGCAACTGCTAAACTTGATATTAATAGTTTTTTCTTAAACATACTTTCTCCTTATAAATTAAGCTTGAACTTCAACTGGTTTTTGTTTAGTTAAAGTAGCTTCTTCTTTTGACAATTCTGCTAATTTTTGTGCTTTGTATTCTTCATAAGTTTCTAAAGAACCATATTTTTTCTTGTTAATTGCATCTTCAATAACTGCAGGAAGGAATGTTAACATTGCAAATATTCCTAATACACATACTAATGCAACTCTAGAAATAATTAAGTTTTTGTACATTTCAGCTCTTGAAGCATCAATAGCTTTTACATATTCTTCAAGTGTTGCATTTCCTAGACTACTGTCTACTGAATAGTCAACATAATCTTTGTTAACGCCTGCAAGTAAGAACATATCAGTAATAGGAGCTAAAATAGTTACAGCTAAACTAAATCCAACTAAGATAACTGAAATTCATGCAAATGGTAAGAAGTATTTTTTCTTATTTTTAATTTCAATTTTGTTTGTTTTTCTATTTTTAATTCCGCCATAAATTGCAACAGCAATAAATCCGAATGTAAATAAAGCTGTTCAGTTAGCCATCAAGTCGCAGAAGGCATATAGTCTTGCCATTGATTGATGCGCATCACTACTATAAATTGTATAATCTGAGTTATCAGCAATATATGCTAAAGCACCAATTGTAGTAAAGATAATAACAACAGGAATTGTGATTACTAGTGAGTATATAATACCAACATATGGTTTGTTAGGATTTAATTTTTTGTGTCATTTTTCTCAGAATGGTAAGTCGCCTGTAGCTAATAAATCTTCTACATAACGAGGAGCTCACATTGAGAAACCATTAATAATACCTAGAACACCAATTGAAATACAAATGTTAATTAAACCAAAAACAATTCGACCAGCTTTTTCATTTTTGAATAAACTACTCATGTATCCATTGAAGCCAAAGAATGATCCACCGTTAAGTGACATTGAAATAGCAACAATTAAGTAAATTAAGGTTGTGATTCCTAAACCAATGAAAATTGCTAAAGGAGTCTTTTTAGGTTCTTTCATTTCACTTTGAATTCCAGCAGCGACATAGAATCCATCATAAGCAAAGAAGATAGCAGCAACAGCAAGGAATACACCAAGACCAGCTCCTAATTTAGGTAAAGCAGTAACACTTTTACCTGATTTAATTGCTTCTGCAAATGTTTGATCTGGAGCAGGCATTGTTCAGTTAACTTCGTTTAAACCACCATTTCCAGTGAATGCTAATACAAATCCCATAATAGTAATAAATACTAATGGTATAAATTTAACACTTAAAACAACAATGTTTTGAACACTACCAACTTTTGATCAAATTGCAGGTACTGTTAAGAAGTAAACAGTCATTAATAAGCTAATTGATAATCAAATAAGTCAGTCAGCATTTGTTCCGAATGTTAAAGCATCTTTATTCATTAATGCCCCAACACCATCTTGAAGTGACATGATTACATACATAGGCATAAATAAGTATGTAAGAGGTAAATAGATATAGGTCATAAAGTCTTTTGAAGCGCGGAAAATCCGTTTGCTGTTAAAGACTTTACATCAGCTCATAAGTGACAAGTTGTCATTTTTAACTGATGAAATTTCAATCAATGATAAAGCCATTGCAATAACCGCAAATGATGCAATTATTCAACAGAAAATGGCTAGAATCAAACTACTGTGCGAGCTTTTTAAAACAGCACCTGATTTAAAGAAGATACCAGCACCAATTGATCCACCTATAACGATCAACATAGCAGCTATAAAGGAAATCTTCTTTTCTTTTGTTTGTTTTTCTTCCATTATTAAATTTCCTTTTTAGTTTTTTAATAAAGTTTTATTTTTTATTTATTGAATTATCCTTTGATAATTCTTGTACCACTTTTGCCTTCGATAGCTTGTGATACTTTGTTTAAGTCAGCAATAACAGCTACATTTTTTGGATTTTCGTTAACAAAAGCAATAGCAGCTTTAACTTTAGGAAGCATACTTCCTGGAGCAAATTGTTCTTCTTTGATGTATTGTTCAAGTTCAGAAACAGTTACTTCTTTTAAAGCTTTTTGGTTTGGTTTACCAAAGTTAACACAAACATGTTCTACTGCTGTAAGAATCACAAATAAGTTTGCTTCAACTTTTGTAGCCAATTTACTTAAAGCGAAATCTTTGTCGATAACGCCATCAACACCTTTATATTGTCCTTTTTCGTCGATAGTAGGTATGCCACCACCACCACCAACTATAACAACATTATCAGCGTCAAATGTTTTTCTAATTGTTTTCATTCCAATAAAGTCAAGAGGAAGTGGTGAAGGAACAACTTTTCTAAAACCACGACCAGCATCTTCTTTAATGGTTGAATTAGGATTAGCCAATTCAGCTTCTTCTAAAGTTTTATAGAAAGGACCAACTGGCTTTGTAGGGTTTTTAAATGCTTTATCTTTAGCATCAACTATTGTTTGAGTCAAAAAGTATAAAACATCTTTTTCAATTTTTTTCTTTCTCAATTCATTGGTTATCGCTGTGAGCATGTGATAACCAATGTAGCCTTGAGACATACCACCTGCTTCTGCAAAAGGTACTAATGGAGTTTTTTCATTAACTTTTTTTGCATCAGCAAAGGCATTGAAGATCATTCCAACTTGAGGACCATTACCATGACCAACAAGCACTTGGTGACCTTCTTTAACTAAATCTGCTATTTTTTTAGCAGGTAATTTAACTAATTCTTTTTGTTCTTCAGGATTGTTTCCTAAAGCGTTTCCACCTAAAGCTATAACAATTTTTGACATAATTTAAATCTTTTAAATTTTAGTAATTGTACTGTTTTATAAGACTATTGGGAGAATTAGTAACCAATAGTTGCTAAAACAACAGCTTTAATTGTGTGTAAACGGTTTTCAGCTTGATCAATTGATCTGTTGCATTTTGATTGGAATACTTCATCAGTAACTTCCATAGCTCCAGTTGCAACTACTGGGTATTTTTTACCAAATTTTTCTTTGATTTCAGCTGAGAATAATGTGTGGTCATCGTGGAAAGCTGGTAAGCAGTGTAAGAAGATAACGTCTTCTTTTGCAGCTTTAATCATTTTCATATCAACTTGGAATGCTCCTAATTCTTTGATACGAGGTTCAAATAATGAGAAGTCTTCTCCTAATGATACTCAAACATCTGTATAGATTGCATCTGCATCTTTAGCAGCTTTGATTTTGTCGTCTTCAAATGTAATTGATCCACCATTACGTTTGAATAATTCTAGACATTTTTCGTAAACTTCTTTGTAACCTTTTCCAGTTTTTACAATTTTTGCTTGTTCTTTAGGTCCGCATAGAACACAGTTCATTCCTGTAAAAGCAGCACCAATCATTATTGAACGAGCAACATTATTTTTGATGTCTCCAGCAAAGACAATTTTTTTGCCTTTAAGATCACCTTTGAATTCCTTAATTGTCATATAGTCTGCCAACATTTGTGTTGGGTGTTCGGCGTCTGTAAGTCCATTTCAAACTGGAACTCCAGAATATTTAACTAACGCATCAACATCAGATTGTTTGAATCCTCTGAATTCGATACCATCATAAAATCTTCCTAAAACTTGTGCAGTGTCTTCGATAGATTCTTTTTTACCAAAGTTAGAACCTGCAGGTCCGATATATGTGCAAGATGCTCCTAAGTCAGCTGCTGCCACTTCAAAGGCGCAACGTGTTCTAGTAGAGTCTTTTTGGAACATGATAGCAATGTTTTTTCCAACAAGTGGACGATTGTTAGCATGTAACCCTTGTCTTTTTGATCTTTTTAAATCAATAGCAAGGTCAATAAGTGCATTAATTTGATCTGTAGTGAAATTTAGAGCTGAGTCTAAACTCCTACCTTTTAAGTTTAATGGCATATTTTCTCCTTAAATTAATTAATAAATTTATTTTTTTGCTAAATTGGCACTACGGCGCAAATTGAGCTGATAAAATTTTATCACTATGATTTATTTTTTCTTAATGTCTTCTCTAATAAGAGGCATTGACATACATCTTGCTGAACCCATGCCTAATGAAAGTTGGTTTCCTTCGAATGATAAAACTTTAACTTTAGCTGCTTTAAGAGCTTTTTCAGTTTTTTCATTTCTTGAATATCCAACTACAACACTAGGTGCAATTGTCAAGTAGTTTGTACCATCGAAGTGAGTTTCGATATCAATATCTAATTGACTTGCTCCTTTACCTGCGATAGGAATTAAGATAGGTTTCATACCAATAATTTGTTCAAGAATTTCTTCTAATGAACCTTCAATTTCGTGTCATACAAAATCAGGGTCATTTAAGTCGATTCTTCAAACTTTTAATACTGATAACATGTTTGGTGAGTATAAGAATTTATTCTTATCTAACATTGTTAATCATGTATCTAAGTGCATTAAGTTTGGCATAGGAGGAACATTGATTGCATAAATTCTTTTGAATTTAACTTCTTTGTCCGCTGCTATTTTACGAGCCATAACGTTAATAGCTTCTTTGTTAGTTCTTTCGCTAACGCCTACTACTAATGTATCTGCTGAGTAGATAAATACGTCTCCACCTTCAATTCTTCCTTCATCTTTTCTGTCGAATCAACGAGGTACTGTATTGTAATCAAGGTTATTGTCAAAGATAAATTCTGAGAATATTGTTTCTCTTTGTCTTGTGACATATTTCATGTGGTGTAATGAAATACCATTACCAACTGATGCAAATGGATCACGTGTAAAGTATAAGTTTGGCATAGGGTCAACTACAAGATCTCTATCTAATTCGATACCAAGTTCTTTTTTGTCGATACCAGCCATCATAACTCTAACCATTTTTTTAGATGATTTAGCTTTAAGTTCAAGAATGTATTCTTTTACTTTAGCTCTTAATGTAGCATCTAATTTTGGTTCGCATTCATCTAATCATCTTTCAATTAATGATTGCTTAACTGATTCGCTAACCAAGTCAAAAGTTTTAGCGAATAATTCGTCCAATTGAATAACTTTAATTCCGTTATCTTTTAATAATTGAACAAATCTTTTATGTTCCGCAATTGCAGAATCTGGTTCCAAAATTGCTGAAAATAATAGTTCATCGAGGCGAGAAGGCGCGATGCGCCTAATTTCGTCTCCAGGTGTGTGAACAAGTACTTCCTTAAGTTTTCCGTATTCACTGTAAACATTAATTTTTTTCATAATTAATCTCCCGATAAAATAAATTTTCATGAATATTAACATATTTGTTAATAAATCAATTTTTGCAATGATTTGTAAGCGTAGCTTATATTTCTTTTAGATATAATACGTTAGTCATTGCATATAAATTATAATTTTTTAATAGGTTAAAAAGGCAAAATTGTGTTAGTTATGGAAATTTTCTCACATTTTGATACATATAATAATATTAATTCTATTTTTAAGTATTAAATAAGCAATAAAAAAGCAAAAAACATAGATATTTATTCGATATCTATGTCTTCTTATTTGTATATTAGTTGTTTTTGATTACTAATTCTAAGCTATTAGGATCAAAATCATAATATTGGTTTTCCTTTAATTTATACTCGTTTTCGTAAATTTTAGGAAATACACTAGCTACTATTTCCATAATAAAATCATATGTCATAATCATAGCCATAACATCATTTTCACAGTATTCTTTTAAATATTCAATTTTAGCTTCTCAAGCTTTGTCACCAATGATGCCTAATTTACGACTAATTGATTCTTCTAAAGCCATTGATCCATTTTGGATTTCAAGAGTTTTATAAGGTTTAATAATATGTCTTAATTTAAATTTATTTTCAGTGATAATGTGTTCAATTTTTTTAATACTGGTTTTGCCTCATAATTCATAAATGAAAGTTGAAAATCTTTCGTCTTTATAGTAAGTTGCTAAATTTTCAACGTTAAAAGGAATACTATTTTTAGAAACTGAAGTATATAAAATTTCTTTATTTAAATTTTCACTATGAAATTTAGCTTGAGGGACATAATTAGTTTTAAAAACATAATAATTTTTATTAGCATCCATGCCATAACGTTCATCGAATTTATTCTTAGATATTGGTTTAAAACAATCTAATAAGTCAATAGTATGTTCATTAACATAAGTAATTAATTGTTCTAATTTTTCCATATCGAGCTTATTTATAGATAAATAGTGTTCCATGTCTTTTTTATGTTGACTATCAGCATAATAAAGTTTAGCTAATTCTATAAGTTCATTGTTTCTGGTATTTTCATAACCTTTGTTATAAACAATGTAGTAGTCAGCTTTATTTGAATAAACAGCAATAATATTGTCTAGAAGATCATAAATCGTGATATTTTTAGGGTCTTTAACAATATTATTTTTATTTGTTATTTGTCCATTAATTGTTTCAATAATTGAAACTTGGTGAGTGATTTGAATATAAGGATTCAAGTAGTCAATTATTGGAAATACTGAGCTAAGACCTTCATAGTCATATCAAATAATTCTTTTGTCTTTTTGGTGTAATAAAGATAATATTTCTAAAACAAAAGATGAAAAGTTATTATGGAATGATCTTTTTTCCTTAACTTTTTCTCAAAATTGATTTAAGTATTCTTGATCAAAAGTTTTTAAACATTTTTTGACAGCAGGAGAATATTGTAATTCAAGAGGTGTAACAGCTACTCTAACTTTATTCATTAAATCTTTGTTTTTACCATAAAACATATCTGAATCCGGCAACATAATTTGATTATCAAATGAAAATGTTGGTTTAGTTAAATAATAAGCTTCTACAATTTTACTTATTACTTCTTTAAAGTCTCCAAATTTACTTCTTGGATCTTTGCTCATAAATTCTAAAGAAGTTTTTGGCATTTTGCGTGCACTTGGTTGAGTGAAAATATCTTCTTGAAGTGCGGCAAAAAGGTAATCTCCATGAACATTAGCTTCATTTTTTGAAGCAAAATAAGAACCATCATTAATTGTGTAACCAAGATCACTTCTTAAAGCCTTTTTGTCCATTTTGTTTTTACCACAACTTGAAGCTTCAATTAATCTTAAGTTTAGTTTATCTTTTCTAAAAACAAAACCATTTTGAGTATAGTCATCAAAAATAATATTTTGAATTTTTTTAACTTCAACTTTGGCTTTTTTAGCTACTTGATATAAGTAATTAGCTTCTAAATAATCTTTATTAGTTGTTTTAGAATTCATTCTTTTAAAATAAATAATTTTATTAACTTTATCATAAGCAAAAGGTTGGGCTTTTATTGTAAATTCTTCTTTATTTTTGCTTAAAGCTTTAAAAGAAAAAACAGGATTAATAATGTATTTAATTTTGCTGTCTTTTAAAGCTTCCTGTGTGTTAATTGTAGCTTCTTTAACGTCTGCTACTTTTTTGAATATGGCTGTTTCATAGTTGTTTTGGACTTTTCAAATGTTATTTTTTAAGAAGTCGATATATTGAGATTCAATCTTATCATAAGCTATTAAATTAGCTCCTTTAAAGTCTCAAGGAGTGTTTTCTGCTTGATAAACAAAATCACTTCAAAATGTTTCTTCTTCACCTTCTTCACTTTCATCATCGCTAAATTGAGCAAGGTTTAAAGAATGATAATTTTTAATTAAAGGCAATGAAAAGTTGTTAGCATCTTTATTTTCTAAATCACTATCTAAAAGATTGTGTCAAATGTAGTATTTTTGCTTAACAAAAAATTTGTAATATTGGCCAAAATTAATTATTTTTTTAATTGAATTCATAATCTTCATCAACTTTTCCTAATGATTTTCAACGATCAAACAATTCTTTTCATTCAGCTTCATTATGGCATCTTAAATAATCAACTAAATCACGATCTTTAGTTAAATTGTATTTAGGAGATTCACCCAATTGTGAATATCTTTTTCAAAATTCAATTATGGTATTTGCATCATCTTCAGCCATTTTTTTATCAACTTTAAAAGGATAAGTTGCAAAATATCTTTCATTAATTGGAAAGTTTTCAGGATCGGCGTAGTCTTCTTCTTTTAAGAATGCAGCTGCAATCATATATTTGTCATAGCCTAATAAATAAGCATAAAGTTGGGCTTGTTTTTTGTAATCAAGAGGTACTTTATTATTGGGAGCATCTCAATCAACCTTTTTCTTTTCTTGAACAGTTTTAACTTCAACTACAATATTTTTTGAAGGAATAAGACCATCAGGAACACCACCTAAAATGTCGTGTTTGCCTTTAAAATAGTCATATCCGACATTTGCTGCTTCAATATGAATTACTTTTGTTGGTTGACTATCAGCACTAACTTTACCTCTTTTAATATCTTTTTGATGCATTTCATTAAATTTATTTTGCATAAGTTCAAAAACTTTAGGTTCTATAGCTACCCCGGCATCTATATATTTAGTTTGTAACACTGGCATACTTAATCTTGACATAAAACAAAAAGCTTGGAAATGACTTTTGAAGCTATCTGTTTCTAAGACGTTACCAATTGAGCTACCACCAAATTTTTTGAAACCATTAAAAGTTCCTAAACTATTTAAAAGTGTGTTATGAAAATTGGTTGTTAATTTAATTGTTTGTTTTCTTTCATCTAAAATGTAATCTTGTCCATTATAGAATTTTCTTTTTGTTAATTTTTTAACTCTGTTCATTTTTATCTCCTTTTAGTTTGTTGAATTTTCTTCTTTATATATTTTGTCTAATCAATATTTTATAGCAAAAATTAAGTCTCTAAAAGTTTCTATTCTTTTTTCAACTTTAGAATCATTGTCATCTATTGATTTTTTATAAAATAAATTATTGCAAAGTTCTCGTTGTAAAAATCGATGAACAACATTATTTCAAAGAATTGTTAAGATTCTTGAATCTTCAAATGAATCATGCATATTCAATTTTTCAAATTTATATTCTTCTTGAAAAAGTATATTTATTTTTTTATCTTTATTTTTTAATTCTTCTTTTAATTCTTCACTAAGTAAAGCATCATCATAAGTACAAAAATATTTTGCCACATTAAATAATGTTTGTCTATACATTGTTTCTTTTTCTTTACTAATTAATTTTTTAATTATCATTGTAAAATTGCAAACATCAATATAATTGACTAATCGACTTTCAACATATTTTTTACCTACAATTTTTTTCAAGTTGTTTAAATCGGCTTTAATGCCATAAGAAATTAAGATTTTGTTAGCATCTAAAATATTTTGAACTTCTTCTCTTGTTATTAAATTTTGACTTTGATCTTGATAAATATTGCTATATTGATCTAAAATGAAACCATCAATTTGGAAAAACTTACGGTTGTCTTTAACTACACCTTTGTAAAAGTTTTTTCTTTCTTTTTCATTAAATTCTTTATTGTTTAAGTTTGATATAAAAGAAGAATATTGAACTGGATAGTAACTAATTGCCACTTTATCATTTAATTTTGTTAATCTAGATTCAAAATCGAAAAATAAGAAGTTTTTATTGTTGAATATTTTTTGATCTAAAAATTCATCGTCGATTCTAATGACATCTTCAACATTAATTTTTTCTAATTCTTTAACACTTGTTACATTTGTTTTATTTTCTTCACATTCAGATATTTTTTGAATAATAGCTTTATAAGGCATTTTATGTCAATTTTTATTTTTTGAAACTGTATAAACAAAACTATATTTAACTAAATCGTTTTGTCTTTTGTTTGCTTCATTTTGTTTTTCTTGTTTAGTTTTAGTTTGTACTTTGAAACCAAATATTTTTTTAATTACAGAATATGATTTTTCTTTATCAGTTTTATTTTTATCTTTTGGATAATAAAAGAATTTAAGTTCAAAGAAATCATTAGTATCAATTTCATCTCCAACTATTAAAGAAATAAATTGATTATTATCTTTATCAGCCGTTAAGCTTTTATTAAATATGTAGCCTTGTGTTTCATAACATTGTTTTTTAGAAAAAATACTTGGGTTATTTTCTAATTCTTCAATAACTTCGATAAAAGTCTTTTTAATTGTTCCGTTTATATTGCTCATATGTCTCCTTTTTTACTAATAATACTATAAAATAGTATTTTTCAATGATAATGGAATAAATAATCATATATGAGCATATGTTAAAAAATCTACCATTAAGGTAGACTAATTTAAATTATTTTAATTTTAAAGCTTTATTTAAAAGTTTAATAACTTGTTCTTCACTATCTAATTCAATAGCTTGAGCAGCCAATTCTTCCATGTCTTTTTTGCTTAATTGAGATACCAATTCTCTTGAAGCTAAAACATTACCAGCAGACATACTAAATTCATCTAAACCTAAACCTAAAAGTAAAGGCAATGCGCGTCTATCTCCGGCCATTTCACCGCACATACCAGCCCATTTACCATGTTTGTGAGCACCATCAATAACCATTTTTACTAATCTTAAAATAGAAGGGTTAAGCGGTTGATATAGGTATGAAATAGTTTCACTCATTCTATCTGCAGCCATTGAGTATTGAATTAAGTCATTAGTTCCAATCGAAACAAAGTCTGCATATTTACAGAATTGATCGCTTAATACAGCAGCAGCAGGGGTTTCCATCATAAGACCTACTTCAATATCCTTTTTAGGAGCAATTTTGTGTCCTGCTTTTTTAACTTCACTATAAGCTTCTTCATAAAGTTTTTTAGCTTGCAAGAATTCTGGAATGTTTGTTATCATTGGGAACATAATAGCAACTTTACCATAAGCACTTGCTCTAACTAAAGCTCTAAGTTGAGTTTTAAAGATTTCAATTTCTTTAAGACAAAGTCTTATTGCACGATAACCTAAGAATGGGTTCATTTCGTGAGGGAATGTAAAGTATTTAAGAGTTTTATCACCACCAATATCTAGTGTTCTAATAACAACTCTTTTACCTTTCATTCTTTCAACTACTTCTTTATAAGCAGCAAATTGTTCTTCTTCTGTTGGTCAGTGTTCATTATCCATGTATAAGAATTCTGAACGGAATAACCCAATTGCTTCAGCATCATTGTCAATAACTGCATCAACATCTTTTGGACGTCCAATATTTGCAGCTAATTCAACATGATGTTTATCTTTAGTAATTGATTTTTTACCTTTGTATTTAGCAATTGTTTCTAAATATTTAATGTATTTAGCTTCTTCTTTTTTATAGTGTTCTGATTCTTCTTTTGTAGGATCAACAATTACTTCGCCTTTGATTCCATCAATAATAATTGTTTGACCATTTTTAACTGATTCTAAAACATTGTTTGTTCCAACTACTGAAGGAATGTTCATGCTTCTAGCCATAATTGCTGTATGACTTGTTGGTCCACCAATGTTGGTTGCAAAACCTTTAACATATTTTTTATTTAATTGAACAGTTTGAGAAGGACTTAAATCATAAGCCACAATAATTACTTCTTTATCAATAGCAGTTAAATCAGGTTCTTCAATGTCATTTAAAATGTAAAGAAGTTTTTTCATAACATCTTTAACATCAGCAGCTCTTTCTTTCATGTAGGCATCGTCCATATTTGAAAACATTGTTGCCATTTCTTCATAGAAAGCTTTTGTAGCATATTCTGCTGTTTCTTTATTGTCTTTGATTCTACCTGTAATTGTTTCTAAAGCTCAAGGGTCATTAATAATTTGTAAATGCGCATCAAAAATAGCAGCTTGTTCAGGAGTTTTAGCTAATTTTTTAGTAGCTTCAACTTTTTCACCAGCTTTTTTAACAGCATCATTATATAGTTTTAATTCATGATCTGCATCCTTAGCTTTTTCAGTAATATTGACTGGTAGTTCTTCAATTTTAAAAACTTTTGCAACCGCGATTCCACGACTTGCTCCGATTCCTTTAATTGTCATTTTGACCTCCATTTTATTTAAATATGTGTGTAATTTAATTATATAAACATAAAGTCTTTTATGGCTTTTTAACTATCTTAATAAAAATTAAATAGGTAATCATTTCCACAAGCAAAACACATTTTTTTTAAAAGCATTTTACATTTTAAATTTTTCATAAAAGCCTGTTTTATAGTTTTTTTAGTTTAAATTGTTAATTTTGAAAAAGTGAAAAATAAAAATCCGCTTACGCGAATTATTATCAGATTTTGACTCTTTTTTCAGGTGCTAAATACATCTTATCACTAGGTTTTGTTTTAAATGTTACATGAAATTCATCTAAGTTTTTAGCTTGAATATTTGCTCTTAAATCCTTAGGCGCATGAACATCAGCAGCTAAAAGCATTTTAGCATATTCAGGTTTTTGTTTAGCTCTTCAAACTGTAGCTCAGTTTGTAAAGAATTCTTTTGATGAATGATTTAATTCTTTTTGACTAGCTTCTAATGCACATCTAATTCCACCAGCATCAGCAATATTTTCAGATACAGTTAATTTACCATTACATTTACCAAATTCTGAATCTTGGCCTTCAAAAAGATCAACCATAGCTTGTGAAAGCTTATTGAATTTTTTGAAATCTTCATCTTTTCATCATGAATTTAAATTACCTTTTTCATCAAATTTAGCTCCATTATTATCAAAAGCATGTGAAATTTCGTGAGCAATAACAGCTCCAATTCCACCATAGTTAGCTGAAGAACTTTGGGTTAATGAGTAGAATGGTTTTGCTAAAATCCCAGCAGGGAAAACTATATGATTCATAAAAGGATTATAGTAAGCATTCACTTCAGCAGGTGACATACCTCATAAATTTTTATTAATTGGTTGTTTGTATTGACTAAAGTTATATTCGCTTTCAATTGCATTAAGATTTAATGCATTAACTACTAAATTACCTTCATGTTTATATGGAGTTATAACCATTAATTCATAAAAAGGTCTAAGTTCACTAGGATAGCCAACATGAACACCTAAAGCATTTAATTTAACAATGGCTTTATCTTTAGTTTCTTGTGAAAGTCAGCTGTTGTTTCTTAAACGGTCTTGATAAATTTTGATCATTTTTTGAACCATATATTCAACATTTCTTTTAGCTTCAGGACCAAAATTTTCAAGTGCAAATCTTAAACCAAAAGGAATTTTAAAGTATGAATAAGCTAAGTAGAAAGCACTTTTAGCTGCACTTGAAGCTTTATCAATTCCACTTACTGCACGGTTAAATTCTCCTGCAGTAATTCTAGTTTTATTGTCCAAAAGATTTGAAAATCTAATTAAGTTTCTAAGATACATTCAGCATTTAAATGTTTCAAATGTTCTTGAGTTATATAAGCTATTTAAACCTTTCATAAATTTAGGATAAATTACAACAACTTTTTCGCTAACTTCTTGTTTAATTAATCTTTCTGCTATTTCTTTAAGATCAAAAACTTTTGAATGTTCATTAATTTCATCAATTGTAAAAGGATTATATAAAGAGGGATAGTCAGCACTTTCAACTGCACTCATTGTGTATTTAACTAATGAGTTATCAAATTCTAATGTATTAGAAATAATATCTTCAATTTTATTTTTATCTTTAAAATAAGCAGCTAATAATTTTCTTGACATGTTTTTAAATACTTCTAAAAGCGGTTGTTTAGCTGGATGATTGTCATCATAATATGATTTTTCTGGCAAAATTAAACTTGGTCCACTAAAACCTAAAGTTTGTAATTCGGTATTTTTAAAATCTTGCATTACTTCAAAATCAAAAGGCAAGTGGAATTTTCTTAATTGATAAAAATCAAAGTTTTCAATTAAATCATCTCATGATTGCATTTCTCAAATTTTATTTAAGTAAGGTTTAAGTGGTTTAACTTTAGATTTATTTCTAAAAGTAAAATCACTAGCCATTGAATAAAGATTTGTGAAGTTTAAAATTGCTCCAACTTCTAATTCACCTGCATCACGAGTTCTAACTAAATCACTAGCCATCTTTCTTAAAATTCTTTCATTGCGGTTGTGAATTTCTAAAAATGCTCCTGTAGCACTTTTATCTCCAGGAATTTTTGCTTTTTCTAATCATTTTTGATTGACTGCAAAATATAAATCATCTTTAATGTTATTTTTTTTCATTAGTTTCTCCATTTCTATATCTAATATTATATTTAATAAAAGATTTTAATTAATATATTTGCTTTATTATTTTTCTTTGATATTAGCTAGCATATACAAACAAGCTGTTTCAGCACGTAAGATTGTTTTGCCTAATGAAGTAATTTTAATATTGTTTTCTTTAGCTAATTCTATTTCTTCAGGACTAAAACCACCTTCAGGTCCAACTATTAAAATTGAACTAGTTGGATAAGTGATAATGTTATTTTTTTGGTCTTGATTTTCATAAGCTAAGTACTTTTGCATAGTAGGATAAGTCTTAATAATTTCTTCTAAATTCATAATTTTAGTTAAATAAGGAATGATGTTTCTAAAGCTTTGTTCAGCTGCATTTTTAATGATTTCATTAAACCTATTTTGCTTACTGTTGAATTCATATTTTAAGATTTTTGCATCAGTATATTTAGAGGCAAAAGGAATAATTGTAGTAACTCCTAATTCAGTAGCTTTTTGCAAAATTCATTCGAATCTTTTAATTTTAATAATTGGTGCAGCAAGAATAATTGGCTTATTAAATTCATGATTTATATCTAATTTTTCGATAATTAAAGCTTTATTATTTTCTAATTTGCACTTATAAAAAATGTCTTGATAGTTACATAAAAATTCATCTTTTTCAACACGTGCAACTTTGATGTGATTTAATATCTCTTTTGATAGTTCAAAATGATCTTGATCACTTTTTTTAGTAACAAAAAATCTATGCATTTTATTCCTTTCATTTAACTAAAACAACTGTTTTAAAGCAATTTAAGTAAAATTATATTTTTAATTTTTAAATTAATTTATATTAATATTTTAGCAAAAAAATAAAAAGAGGATTAAGATAACCCCCTTCTTTTCTATTAATTTATAAAATACAACTTTAACTATTAATGATTTTCATCAAAGAAATTTGGCATTACATCTTCATTTTCATCTTCATAGTTTTGAGCAGTATAACTCATTATACGAGTATGATGTGATCCATTAATTTGTGTATATGTATTTTGCATATTTTGCTCATAATGTTCACTAACATTTCTTTGCAATAATTTTGGTACCGCATAAGTATTTGTTGAAACATTTGCATTAAGATTTGAAGCAATAATACTAATTCTGAATATTTCTTCACGATCTTGAGTATCGATATATTGAAGTCCGATAAAAGTTTGAGCTTCAAGGTTATTTGCTAAATACTCATCAATTCGATTTTTTGCTGTTTCAATGTCATCTAAACTTGCTCTTTTATCACACTGAAATAAAATAGCAACTTTTTGACAATTTTTAATTTCACTTTGAAAAAGTGGAGTCATTAAAGCATCATCAACTGCTTTAATAGCTCGGTCTTTGCCAAAGCCATTACCAATTCCTATAATTGTTTCTTTTCCATCTAATAAAACTTGACGTAAATCATTGAAATCTAAATTAATAAACCCAGTTTCAAAGATAATATCACGAATTATTTTGATTGAATTTTTCAATGTATAGTTAGAAACTTTAAAAGCTTCTTGAACTGGTAAGTTTCTGTAATTTTCAACCAATTTTTGGTTTGAAATTACAATATAACTATCAACAACTTCACTTAATTTTTTCAAGCCTTCTTGAGCAATTAAGCTTTTATGTTTTCCTTCAATTAATTCAAAAGGAGTGGTTACTACTGCCACTGTCAAAATGCCCATCTTTTTGGCAGCTTCGGCAATCACTGGTGCTGCTCCAGTTCCTGTTCCGCCGCCTAAGCCAGCTGAAATAATAACAATATCTGTGTTTTCTAATGATTTTTGAATTTCTTTAATGCTTTCTAGTGCGCACTCACGTCCTACTTTAGGGTCCCCACCAGCACCAAAGCCTCTGGTAGAATCACCTAAAAGTATTTTATTTGGACATAAGCTTTTAACCAAATCTTGACGATCTGAGTTAGCAACTAGTAATTCAACATTTGGCAAGTTTTCATCAAGCATCAAGTTAATGGCGTTATTACCTGCACCACCAACACCAATGACTTTTAGTTTAACTTCCAAATCTTCAGCATTTAAGTCCATAGGCAACCTCCTTATATTGTTTTATTTAATTTTGTCTTATTTTTGAGCTCTTTGTGATGGCAATATTCATGAGAATAATTTGTTTCAAAAACTTTTTGGTTTGTATTCTGGTAAATTATTCAAAGTATTAACCATGTCTTCACAATTGATTTTTTCAGGATTTAGTAAATTAATAATGCCTAAAAGGCTATTAGGTATATTGAATAAAGTTGAGACGTTATCGTCTAGAATTGCAACATTCATTTCGGGATATAATTTGTTAAAACGATCCATAAAGATTTCTGAGTTTTTGCAGTTTAAAACCAACTCATCAACTTTTTGATTATTTTGGTCTAAAAACGCTTTAACTTTATGAATAGCTTGGTCAAATAAAGAAATCACTAGTCTATAAGCTTTATAGCCTTCAGTTTCATCATTTAAAACTTGGTAAACTTTTCAATTAGCTACCACAGCTTCAAGTAACAAAATTGTATCTTCTTTACTTTTATTTAATTCTTTTGATAAATAATTAACAAAGTTTTTAAAGTCAATGTTAGATTTTTCATAGAAAAATGTTTTAGCATTCATAGAGCCATTTACTACTAGTGCATTTCAGTCAAAGTGGAATAAGTATTTAGCTTTATCTTTTTCTTCTAAATTTGCACTAAGAGCTTGAGATTCTAAAAGTAAAGCATCAAGTTTCAAATTACATTCTTTAATAATGTTAATAATTTTTGCAAGTCAAGTATCTTTTGTAATAACAAAAAGTCCTTGATTAATATTTAATTTTAGAGCTTGTTTGTTTCAAGGAAAAGTACTGTAGCTTTTAACAACATCATTGTTAATTGTTTCATATAAATATATGTTTGAATTAATCAATTCGTATGTTGAACTTTCTTTTTGTTCTTCAAGTTTATTTTTAACTTCTTTTCATACTTGTTGATCAACTGTATTACTTGGAACATCAAAAGTCAAAGTTTGGTTAACAAATTTTAAACTTTTGTATAAACTATCTTTAACTATAAAAGAATATTTAACTACATCATTTTTCTTGGTAGCAACTTTAGATTTCAAATATTTTTTGAAATTCGTAATAAATTCTTTAATCTTATTCGCTTCAAAGTTTTTGGTTTCTAGATATGTTTGAAAAACTGGAAAATATTTAGAATTAATATCTTCCACTACTGTCACATCAAGTCTAGTTTTTTTAAGAAAGAAATTTACATAAATTTTTTTCATAATTACCTCCAAAATAGTCTTTTTAGCAAAAAAAGATAGATTAAAAAAATTAAAAGCATTTTTTCAAAACTCTTAACTTGGCGCTACGAGAGCGTTTATTAAGTTCTAACTCATTATTGGTTGGAAGAAATACTTTTACTTTATATTGCTTCTCTTCATTAATTGGCATTTTTGCTGGTAATTTACTTTTAATTAGTGAACCAAAAAAATGTTTAACAATGCTGTCTTCCAATGAATGAAAAGTTATAACGGCTAAAGAAGTATTAGGTTTTAATAGTTCAATAGCATCTTGTAACATTTGCTTAAGGGAATCAAATTCATTATTAACTTCAATACGGATAGCTTGGAACACTGCTTTATTAGGATTTTTTAGTTTCACTAATTTTGCAGGATAAGCATCTCGCACTACTTGAGCTAATTCCAAAGTGGTAGTTATTGGCCGCTTACTAATGATGGCCTTCGCAACCTGACGAGCAAGTTTTACCTCAGCGTAGTTTCTTAATAATTGTTCCAATTGACTTTCACTATAATTATTAACTACATAGTAGGCATCAAGCTTTTGACTTTGATCCATGCGCATGTCAAGACGTGCATTTTTGTTATAAGAGAAACCTCGTTCATCATTATCAACTTGTGGACTGCTAATACCTAAATCAGCAATGATTCCATCTACTTGGCTAATGTTCAATTTATCTAATTCACTTTTTATATTTTTGAAGTCAGAATGAATTAGTTGAAAATTAGTTGATTTTATTTGGCTGAGACGTTTGCGACTTTCTTCTATCGCAAAAGTGTCCTTATCAAAAGCAATCAAACGACCAGTGTCAAGTCTCTTTAAAATCTCGCTAGAATGTCCACCCATGCCCAAAGTTAAATCAACATAAATACCATCTGGTTTTATATTAAGGGCATCAATAGACTCTTGCAGTAAAACAGAAAAGTGTTGATTCTCCATTTTATTTACCACTTCTTTCAAGAATTTTTTGTGCTGCTGAAGATATGTCACTGTCATTAAAGTTTTGACAATATTGATCAAATTTTTCGCTGCCTCAAAGTTCTACAAGATTACCTGAGGCTACAAAAATGGCTTCTTTTTGGATAGCAGCTTTGTCGAGGAATTGTTTTGGAACAACGAAACGTCCTTGGTTGTCAAGTTCAATTTCTTGAGTATTGCTCAATCAAAATCTTCTAACAAAACGAGCATTTGTATCAAAAGAGTTTTGTTGATTTAGAACTTCAACGAATTTATTGAATTCTTTTTCGCTTCTAATTTCAACAATGCCTTCAAGTCCGACAGTTAAATAAATTTTGCTTCCTAAATCGTCACGCAATTTAGATGGTAAAGCTAATCTTTTTTTGTCGTCAATGTTTCTTTTGTATTGTCCAAACATTTTCCACTTCCTTCCACTATCCACCACTATTTTACACTATATTAAATACATGTGCAAGAAAATATTTGAATTTTTTCATTAATTTAAACAGCTTTTGCAAGCAACTTTTGAAGGGGCTATAACTAAAAAATGCGTTTTTCAGCGAATGTTCGCTGAAAAACGCATTTATCGAGTATAAAACCTTCAAAAATTTTGTGAAAATTAGGCAAAAAATTATTTTTTAGAATATGTGGAGGACTTTCCCTAGTCTTCGACATTTAAGAAACCAAAAGCATTGGTTACATCTTCAACCTTACCTAGAATTGTAATAAGATCACTTTTTTGTAAAACAGTTACCCCAGTAGGTAAAATACTTTCAGTTCCTCTCTTAATTAAAACAACTGAAATTCCTAAGTTAAAGAATTTTAATTCTTTGATTGGTTTATTGATTAATTTTGGATTATTTAAAATAGTAGTACCCATAACGAAGCCATCGCCAATTTCTTGTAATGTTTGAGAATAACGGCTAAAATTTGGATTAGCTGCTAAAAGAGCAGTACGAACACCGGCTTCATATTCAGGTCTAACAATAACATTAACTCCAATTTGTTTTAATACACGTGCATGTCTTTTACTTGTTGCACGAGCAATAATATTATTAACTTTTAATTCGAGTAAAGCAGCCACAATTTCAATATTATCAGGAACAGCAACAACAACAGTTCCAATTTCTTGAAGATTAAGTGCTCTTAAAGCTTTCATATCACCTGCATCAGCAATAACAACTTTTTGTGCTAAATCATCATATGATTTTAAAACTTCTTCATCTTTATCTATTAATAATAAAGGACAATTCATTTTAGCAAGTTGGCTTACAACCGCACTACCAAAACGCCCTGTTCCAATAACACAGATATATTCATTTTTTCTTTTACGAGGCATAATTTACTCCTTTGTGTTTTTATTAACTTATGCTATAAATTATAAAAGATTTTTATTTTTTTATAACTAATATTTTAACTCCAAAAATTTGGCAAACTTTTTTAAATAAAAAAGAAAATAGTGCTCTATTTATTAATAATAAATAAATTTCATAATTTTAAAATTAAGTTAAAATATATGTTATGAAAAATTCAAAAATTGCCGCTTGATGAAGAAAAAGCAAAATTAGACAATTTTTTGCAAACTATAGAGTTTGAAGATTAAATATCACCAAAGTTAAATACATCTTTTTAGCTTATCTTCTTACTGTTATTATTGCAACACTTTTCCTTTTAAGTCCTTGAACTCATAAAGATAGTAGTGTTAAAGTTAGTTTTTGAGATGCTCTTTTTACAACATCTAGTGCTTTCAGTGATACAGGCTTAGTCACTAAGACAACCTATAATACTTGAAATATGTTTGGACAAGCAATAATTGCAATCCTGATCTTTTTAGGAGGATTAGGTATTTTTGCCTTAAGAATTTTCTTGATTAATTTAATCTTTTTTAAACGTAGAAATTCTTTAAGTGAGCTTGAAGTTGTGTCACATGAAAGAGGCAGCGGTGATAGTGGTCAAACTAAAAAATTAATTATGGACTCAATTGGAACCTTATTAATTATTTGAATAATTTTTAGTTTTGGTTTAACTTTCTATTTCTATTATAATGAACCAAAAGCTTATAGCGATTTTGATAAATATGGAGATTATATTTCACCTTATAAAAATTGGGGCCTAAGTTTTAGATATGGTTTCTTCCATTGTATTAGTGCCTTAAATAATGCAGGCTTTGACATTATTGGTAAAAATTCACTTATGCCTTACTACCACAATATTGGATTACAAATTATTTTCTTGACTCTTTTAATAATTGGTGGTTTAGGTTATCCTGTAATTCATGATATTTTAAACTTCTTTAGATTCTTAATCAAATACAAAGGTAAAAGAAGATATCAATGAAGACTATTTACAAAAATTAGTTTATAACTTACTTTGTCACAACTTTAGTAGCCTTTATCTTAATTATTTCCTTTGAAGGTACTTTTAAAAATGATAATTATTTTTGAAGAAGTGATGATAAGAATTTAGGCAATAAATGAGAAAAAGCTTGAGCATTATTATTTACAACTTTTTCAACTAGATCAGCAGGATTTTCAACTATTAATTTTAGTAAACTAACCTCTACTTCATTAATCATTTGTTCAATTTTAATGTTCATTGGTGCTGCTCCAGCTTCAACTGGTGGCGGTATTAGAACTACCACTATGGCAATATTATTCTTATCATTATTCTCAAAAATTATGGGTCGACCAAGTGTTAGAGCTTTTAGAAGAAGAATTGATGATGAAACAGTTAGAATGTCATCAATAGTTTTCTCAATTAGTATTTTCTTAATAATATTTATTTCATTTATTACCATGTCAAGTTTAAACAATTACAGTGGACAAATCGATTCAAACAAATATAATTTCATTCATATTTTCTTTGAAGTTAACTCAGCATTTGGAACTACAGGATTAACTTGTGGTATTACTGATAAATTAAATAATGCTTCAAAAGTTTTCTTAAGTATATTGATGTTCATTGGACAATTTGGTATTAGTAGCACAATATTAGTTTGAGGTAAAAAGAAAAATTATTCTAACAAATATGAATATATTTCAGAGCCTGTAGCAATTGGATAAAATTATGCAAAATAATAATTTATTAGATTTAGGAATAAAAACAGAAAAACTTGAAAGATGAGCAAGCTATAGCACAAATAAAAAATATAGAATATTAGTTTCAGTTTTTTCAACCTTTTTATTACTAACAATAGTTTTATGTTTAATCTTTATTTTTATCTTTAAACATGAAACTAAAGTCTTAATTTCTTTAAGTATTGTTGCTTCAATAGCTTTAATAATTTGATTCTTATTTTTAGCTCCTTTTACTTACTTAATGATCACTTCATTTTGAACCTATAGAGCTATAAAACAGCCAGATAAACCAATTTATAGAAATTATAAAGAAGCTAATTGGTGAATTAAAATACAATTAAATTATGCTAATTTTGGCTTTAAAATATTTAACAAGAAAGCTTTGCATTTAACTAAAGAAGAATACAAGCTTTTTGTCAATTTTTACATGAATGTAAAATAATAGTTTTTATATTTTGCATTCTTTTTTATATAATCTAATTATTATGAAATTAAATTTATTTGCGTCTGAATACAAATCATGACAAATGCTGAACAAAAAATTATTGATTATATCAATGAAAATCCTAATGAGTTTGCAGAAAACTCAATTGAAAAAAATGCTGTCGAGACTTTTGCAAGTACAGCCGTTTTATCTAGAATTTACAAAAAATTAGGATTTTCAAGTTATAAGGATATGCAATTTTATGTTAAGTATAATTATTTGTCAAATTTAAAAATACCGAAAGAATTACTAGATAATTCTATTTATAAAATAGCTAAAACTTATATGGAAGCTATTAATTTAACAAGCAAATATTTAGATATAAAAGAAATTGATTATATTTGTGAAGAAATAAACAAAAGCAAACTGGTTTATTGTTTTGGAATTGGTTCAAGTTCAATCAGTGCTAAAGAATTAAGCTTGAACATTGAAAAATTTGATTACTACAGTGTCTTCCATAATGATTTTCATAACTTCTTAATTTCTTTATATAGTCATGAATTAAAAGAAAATAAACCACCTGTTATTTTGTTTTCTAAAAGTGGTAAAACAAATGAGATTTTGTTTTTATGTGAAACATTATTAGAACACAAATTTCCATTTTTATTAATAACTGCAAATAGATCACTTAAAAATAAATATCCTCACGTTTTATTGCATGAAACAATTGAACAAAAAGAAAGATATCATGCTTTAAGTTCAAAAATTGTTCAACAATATATAGCTGATATTATTGCAGATCATTTAATTTCAAACAGTCCAAACAAATTAAATCGTAAGAATTGATTAAAGATGATTAATGAATGAAATAATTTAAAAAAACACGATTAGCATAATCTTACTAACGTGTTTTTTTATCATTATTTTTTATTGCTTAAATAGTCAACAAATTGTTTTGTTATAAAAGTTGGTCTAGTAATTGCTGAGCCAACAACTACACCATTTGCACCTATTTTATAGGCTTTTTGGACCATTTCAGGTGTATTAAATCCGCCTTCAGCAATTATGTATTTTTTGTACTTTTTATTTAATTCAACTACTTCTTTTAGAAATTCAAAATCATTGTAACTATTAGGTTTATTTAATGTGTCTTTTGTGTAGCCTCTAAGAGTAGTTGAAACAATATCAAAATCTAATTTAAAAGCATTTTCAACATCTTCAATTGAAGAGCAATCTGCCATTAATAACTGATTATGATTATTTTTCTTGAAATATTGAACTAAGTCTTCCAAACTTTCTTTTGGTCTTTTTCTTAAAGTTGCATCTATTGCAATTATTGGACATTTACTTTCAATTAAGAATTTTAAATCATCTAAATCAGGAGTAATAAATACTTCAGAATCTTTAGACATTTTTTTAGTAATACCAATAACAGGAATGTCTTTAGGTACTATTCTTAAGACTGGTTCAATATTTTCTCTTTGGCTAATTCTTATGCCTTGAGCACCACCTAATAAAACAGACTTAACCATTCTTTCGATTACACCAGGACCATACATTGGCTCATTTTCCAATGCTTGACAAGAAACTATAAACAAATTATTTTTAAGACTCATCACTTAATTTTTCGTTAATTTTACTTGCAACTACAGTTGCTTTTCCGCCAAGTATTACTTGAACTGATAAATTATCATGAACTAAACCAAATGATCCATATGGTACAAAATATTCTGCTTTAATATCAACATTTTCTTTTAATTCAATTCTTAATCTAGTTGCACAAGCAGAAACATTGTTTAAGTTTTTAAGACCGCCTAATTTATTAACAATACCTTCAATATTAGCATCGTCAAATACTTTTTCTCCGCCTGCTTGTTCAATTAATTTTCTATCAGCTTCTTGTTGTTTTAATTCTTGATATTGTTTTTTATTAATTAAACCAATATTATTTCCAAGACGACCTGGAGTTGCGATTTTGCCATATTTAATTCAGAAGAAGAATGTTACAAAGGCAACACCTCCTAAAACAAGAGCTCATAAGAATGCAAAGTAGAATGCAGTACCTTTAGCAACTGGAATAACACCATAAATAATTAAGTCAATGAAACCACGAGCAAAACCAACACCAACGTGAGCTCCTGTTCATTCCATTAATGCATATGCTAAACCACTAAGTGGTACATAAATAGCATAATAAAGTTGAGGAGCAATAAACAAGAATGTAAATTCAAGAGGTTCTGTAATACCTGTTAAGAATGCAACACCCATTGCTGAACCTATTGTTATCGCTACATTTTTACGATTTTCTTTTTCAGCTAAAAGAATCATAGCTAAACCAATACCCATACATGTTCCAATGTAGGTTGGATAGTCTTGGGTAAATCTTCCGGCATTAACTCCAAGAGTTTTTTGGAATCAAGGGAATAATTTAATATTATCAATGCTATTGTAATTTAATTGATTAATGTAGTTTCAGATGTTTTGGTCACCAGTAATAACATCTTTTCCTTTAGAAAAACTCTCTAATGAATTTTTTAAATCAGTAGCTACTTTTGAAGAAATTTTACCAGCTGTTACAGCCGCATCAATCATTTCCTTAGATAACACACCACCAAATTCAGTTTGATATGCTAAAACAATTGGAATGTGGTGTAAACCAAAAGGCATTAAAGCACGGCCTAATATACCATAAATAAATCCATCTAAACCTATTGGTGATCTTTGCATTAGTTTACCTAATTCATAAATTGCTAAACCAATTCATGGTCATAATAATAAGAATACAATAGATACACCAAAAGAAATAGGTACTAAAATAATTGGCACTAATCTAATTCCTGAGAAGAATGAAAGTGCAGGTGGTAATTCAATTTTAGAGAAATAATTAAATGTATATGCAGTGACAATTCCAATAACCATACCACCAAAAATACTTGTATTTAATGATTTCATTCCTAAGTTACTTCCTACAAGTCCAGCAACTGGTTTGTGGAATCAAAAAATACTTTTAACAATATCAACACCATCAAATTGGATGAATGCTAATTGAGAACTAGTAAAGACAAGGTAAGCAATAGCAGCCGAGAATCCTGCTCCACCTTTGTCTTTTGAAAATGTGATCGCTATGGCAATACAGAATAAAATAGGTAAGTTTCCAAAAATAACTTCAGATACACCTTTGAAAATATTAGCAGTTGTTTGACCAGCACTATTAGTTGTATTTGCACCAATAGCTCCACCGATTCCTAATAATAAACCAGCAATAGGTAAAATTGCAATAGGCAACATTAAACCTCTAGCTAGTTGTTGAAATCAATCTTTGATTTTTCCTTTTTTAGGATTGCTCTTAATTTCTTTATTAGGAATTTTAGCTTGAGCTTTATCCATAAGCTAACCTCCATATTATTGAAAAATGAATTTTTTTCACTTTTCACCATAATTATATTTAGATATAAATATAAGAATATTAATTTGTAAAATCCTTACAAAAGAATACCAAAATTTTGTATATTTTTTTCACCCTAAAAGACAAATTTAAAGGATTTTAAGACATTTTTTACACTTATTTTGCATTCATTTTTTATTAGTATAAAATTAGATAAGATAAGGAAGTGCAACATGAAAAATATTGTTACAGAAGTAGCTGTTGATTCATATCAATCAGCATTAAATGCAGATTTAGCTAAAGCCAAAAGAGTTGAACTTTGCTCAGACTTAGTGTTAGGAGGCTTAACTCCTTCGGTTGGTTTATATAACTTAGTGAGAAAAAATACTAAGCTTGATATTGTAGTTATGATTAGACCAAGGTCAGGAGATTTTTGTTATAGTGCTAGTGAAATAGAACATATTATTGAAGATATTAAAGTATTTAGCAGTTTAAAAGCAGATGCTTTAGTTGTCGGAATTTTAAATGGTGACTTTAGCATTAATTTAGAAGCTATGAAAAAAGTAATAAAAGCTGCTAAAAATACTCCAATAGTATTTCATCGAGCTTTTGATGTAGTTAATGATCAATTTAAAGAATTAGAAAATCTTAAAAGACTTGGAGTTAAAAGAATTTTAACTTCAGGAGCTAAACCAAATTGTGATTTAGGTCTCGAAAGAATCAAAGAATTAGTAAAAGTAGCTAATGGCGAAATAACTATTATGCCAGGAGCTGGAGTTAATGCTAATAATGCTGATAAAATAGTGCAAGAAACTAAAGCTACTGAAATTCACTTTACTGCTAAAAGCATAGTAAATACAAGTAGTTTGGACAAAAGTAAGGAAGTAAATTATTCTTCAAAAGAATTAAATGAACATCTAAAAATTGAAAGTGAAGTAGCTAAAATTACTGAAATAATTGATAAGATTAAAGCAGCTTAAAAAGCGATAAAAATTGAAAATAAGAAGCAACAAAAAACAAGAAAAAAATATTTTTGTAAAAGTCTTGTTTTTTTGTTTTTTTATTATAAAATAATAATGAATTTTTATAAATTCAAATTTGATCAAATTTAAATTAAACACACTATTATGGATTCACTTGGGTGTGCCTGGAGAAAGGGTGCTAGGTGTTAGAAATAATAGGAAGTATAACAAACTAAAGGAAAATTTTATATGTCAGAAAAAAAAGAAACAGCAGTTCAAGAAACTGCAAAAGTTGAAAAAAAATTTATCGTTTCTAAAGATAAATTATTAGAAGCTGGTACATATTTTGGCCACAAAGCAAGTATGTGAAATCCTCGTATGAAGGAATTCTTATTACCACAACCAAAACGTGGAGTACATGTTATTAACTCAAACTTCACATTACAAAGACTTGAATTTATTTACAACTTATTGAATAAATTCTTAGCAAAAAATCCACATGCGTCATTTATTTTTGTTGGTACAAAAAAACAAGCACGCGAAACAATTAAAGATAATGCTTTAAGAATAAATAGCTACTATGTTACTGAAAGATGACTTGGTGGTACATTAACTAATTCTTCAACAATTTTCAAAAGAGTTAAAACTATGGAAGAATTAGAAGCTAAAGCTGCTAAAGGTTTTGAAGGTTACACTAAAAAAGAAGGTCTTTTATTCCAAAAGAAATTAGACAAACTTCACAAAAACCTTGAAGGTATTCGTCGTATGCAAGGACTTCCAACATTTATGATTGTTGCAGATCCTAATGATGACGAAATTGCTGTTAAGGAAGCACGTAAAAAAGGTGTAAAAATTATTGGTATTTTAGACTCAAATGCTAACCCAGATTTAGTAGACTTTGGTATTCCTGCTAATGATGATTCAGCTAAAAGTATTACATTAATTATTACAATTTTAGCTGATGCTATTGCAACAGCTAGAGGTGGTAGAGCTCGTTTTGCTTTCCAACCTGATGAAAAAATTGTTTTACCAGAATTCAAAAGTGAAAGAAAAGAAAACAATTTTGTAAGAAGACCATTTAACCATAACAGAAACGAAAATCATGAAGCCGGTGAAAGAAAAGCTCACCATTCAGAAAATCATGAACAAGAAGTTAAAGAAGAAAAATCTTCAAAAGGCAAAAAACAGTCAGAAGAAGTAGTTGCTAAAAAACCAGATACTAAAAAAACTGCAACTAAATAGGAGTTACTATGGATAAATTAGGTTTAATTAAAGAATTACGTGAACGTACCAATGGTGGTATGGTTGACTGTAAAAAAGCTTTAGAAGAATCAAACTGAGACATTGAAAAAGCTATTACATGATTAAAAAGTAATGGAAAAATTAAAGCTGCTAAAAAAGCTAACAGAGTTTCAGCTGAAGGTTTAGTTGCTATTGCAGCTAATGCTACTAAAGCTGTTATGGTTGAATTAAACTGTGAAACAGACTTTGTTGCACGTAATGAAAAATTCAAAGCTTTAGTAGAAAAAATTGCTAAATTAATTTTAGATTCTAAAGCTAAAACAGTTGAAGCTGCTCTTAAATTAAAAGATGGTAAAGAAACTGTTAACTCATTATGTGAAAATTTAACAGCAACAATTGGCGAAAAAATTACACTTCGTCGTTTTGAAATTGTTGAAGCTAAAAAAGGTGAAATTTTAGGTCACTTTGTTCACGTTAATGGTCAAATCGCTGCTATTGTTAAAGTAGCAGGTAGTCATGAAGAAAGTGCAAGAAATGTTGCAATGCACTTAAGTGCTATGAAACCAGAATTTATTTTTGCTAAAGATATTCCTGCAAGAAGACTTCAATTATTCAAAGATGAATTTGTAGTTCCAGCAAACTTTGACAAAAAACCAGAAAAAGTTCAAGAAATGATTCGTCAAGGATCACTTAACAAAAAAATTGGTGAAGTTACTTTAGAAGAACAACCATTTATGATGGATGAAAGTTTAACAATCAATAAATACTTAGCTAACCATAAAAGTAAATTAGTTTCAGCTATTCGTTATGGTGTAGGTGAAGGTATTGAAAAAGTTGCTTGTGACTTTGCTTCAGAAGTTGCTGCGCAAATGAAAAAATAATTATTTTTTAAAGTTACCATTTGACTTTTCAAATGGTACTTTTTTTGTTTTTAAAAATTATTTTAAAATCAAAAAAGTTATACACAAACAATCAACATAAAAATTGATATTGGATTTTGACCAATTAAACTTATTTAAAGGTCAAAAAGTAAAATAGACAAAAACTTATGCACAATTTTCAAATTAATACTTTTTTGGAAAAATGGAATCATTTTCTTTTATACAATTAAATCATGGAAACAAAAATAACGACGAGTAACGAACAGTTACCAATATTGAAACCTATTTTATTAGCATCAGATTTCGTCGAAAAGATCAAAGGAGATAAGTTATATTCAGATGTGATGATGCTAATAGCACAAGATGAGGATTATGTCTATTTTGTCTCATGTTTAAAAGAATGAGAAAAGAATTATGAATTGGAACCTCGTTTTATTAAAATATATGAGGAGGAAGGCATAATGGAAAATGGTGATAAAAAACCAATTACATTAGCTAAAGGAATTGATTTAAGCACAATTTTCAAAATTAATTTCCATGAACTTATAGAAAAGGTGGGACATAAATTAAATCAATTGCCGGCATTACCTTATTTAGGCTTAAAAAATCAATTGGAATTTGTTAACAGGATAACAGAAAACATTAACGAGGAAACTAAAGCCAATGCACCACGAATTGTTATAATTCGCAGAAGAGCAACTCTAAATGATTTTGATGTTAGAGAATGTAAAAGAAAATTAAATCAAGTTCAAGACTTTTAATTTTTCTATACACTACGATATTTTGAATATCGCTTTTGGTATTGGGCGATATTTTTTTGTAAAAAAATGAGCCTAATTCAGACTCATAATTCTATTATTCGTATTGGTGCATATCAACATTAAATTTACTCATTACTTCATAAATTTCATCAGCATTTTTGGCTTTCATAATTTTATTATCCAAAGTATTATCTAAAGCTAAAATACTAATCATTTGTAAAGTATCAATTTGTAAATTACGATTATTTTTACTTAATACTAAAGCAAAAACTAAATTAACATTCTTTTCATCATCAGCTTTTCAATCATTAACCCCATCATTATTCATAGTTAAAATACATAATGTGTCTTTTTTAACTGTTGAGCTTAATGCTTGACAAATTGCAATGCCACCGCCTATTGCAGTATTCATTAATTCTTCTCTATATAAAAAAGCTTCATATATTTTCTTTTCATCACTAACAAAGTTTTGTTCATTTAGTTGATGTGATAAAAAGTGCAAAACATCATCTTTTTTATCAAAATTTGAATGTACAAAAATAGAATTTTTGTTTAAAAAATTATTGAATTTCATTTTAGTTGTTCTTTCTATTATTAAATTTCTCTTTTTTCTAATCTAGGCATGATACTTAAAGAGAAAGCTTCAGGACCTGTATGAACTGCGATGGCAGAAGATGTTCATCTTTCTGTATCTATTTTAATATTTTTTTCTTTTGAAACTTTACGAACAGTTTTATTAACTTCTTCATCAATTCCGTGAATTCAGTCAATTTTGAAGTTTTCTGTTAATGTTTTGAGATCTTTTGCTTTAACGAAAGCTCCTAATTTTTTAAATATTTGAATTATTGCACCCTTAACACCACGAGCTATTCCAGCTGTTGAAACATGTTGTTTAATAAACTTAACATAAGGATAAATTCTTAATTTAGACATTGTTTTTAAAACGAATTTTTTAAAGCTATTATTAACTCTACCGCCCTCAATCAAATAATTAAGTTTTATTGGCAAAACGTAAATTATTGAATTGTTTTTTATTCATTCTAATTCTTCAAATACTTTGTCTAGTGTTTGATATTTTTCATAAACTTTTTTAATATGATTAATAATTTCATTGTATTGGTCACCAACTCAGCAGTTATCATAAACGTGAAAGTTTTTATATTCTTTGCAAATTGTTGCAATGTTGTTGCAACTTGAAGATAATGATGAACCAATTGGGAAGTAAATTACTTCATCATATTCTTTAGTCATTTTGTCCACTGTATCTAAAACAGCTTTAACATTTGGCATAGATGATTTAACTGATTTTCCTTGATCAAGTTTATGCAATAAATCTTCTTGGTCTAAGTCAATACCATCTTTATAAACTACACCATCAATATCTGTTAAAAAAGGAAAATAACCATAACCTTTTTTAAGAATTAGTTCTTGCGGTTCACTAATAAAGCCGTCAACAATAAAACCTATTTTTTTCATTATTGACCTCCAGTCTTTAAATAAAAATACTTATCTATTATAATATACAATTATAAAATTTTATAAATAATTTTAATATCATTATATCATTAATTTTTATGCATTTTTATTTTAATTTTTTAACATTTTTAATTTAGACTAAAATGCCTATAAAACAATAAAAAAAGAAAAAACACATGGTTCATCTTTTTTCTATTTGCAAACATATTGTACATCTAATGTTTTTACCATAAGTTCATCTAAGCCTTCTGGTAAGTTTTGATAAATAATAACATGATTTTTAATAGTGCTAATTGTTTTAACGAAGTTTAAAAGTTTTATGTATAAAATTTGATCTTTTTCAATGTATTTGCAAATCTTTTCCGATATAACAAAAACCTTGATATTTCCGATATTAACATAGTTAGTTAAACGGTCATTTATCTTTTCGATATATAAACCTAAGTTAATGCCTTCTTTCAAATTACTAAAAGCATTGTTAATTAATCGATAATTAAATCCGTTTTGTAATGGTTTAATTAATAAAGTTGTTTTTGGTCGATCATATGTATTTAAATTAATTAAGTCATATTCATTTGTTTCCAAAATAATGTTTTCATCTTCTTCACAATTTTCCATTAAATATTTGTTTCATTTATTGGTATAAATCAAACGATATCACGAAACATTATTAAAGAATTCTGAATCTTGGTCAGAGTAACCTATGACTCTATTTTTTAAAATTGCAACTTTGCTTTGACGTTTAGTTTTATATGAAATAATTGGAATGTTTTCTTTTATATATTGTTGCAATTCATTTTTCTTTTCATAATCAAGCATCATAGATTTAAATTCTTGATAATGGTTGTCATGTTTTGTATCTGAAATTAATCAAGAAGAATATTTATCAAATTTGCGTTTGTTATTTAAGTGTTGTATTAAGTATTTTGATTTATCAACTAATTCTTGAACATCATCAACACTTTTGAAAGTGTTAACACTTAAAAAAGCAAAGCTATTGAAAAATGGATTAATAATTTTTCTTTCATGTAAGAATTTTAATCTTTTAAGTGCTTTTTTAGTGTAGTGGTTTAGCCAGAATGACGAATTGGTTTCAAAGATTAATAATATTAATCCGTTTTTAACGTAGTAATATCCAAACATATTATTAAGCTCAAATATTCTCAAAATATCTTCAATGTCTGATTTACTAATGACATTATTGTAGAGAAATTCATTGATGCCCAAAGCATAAGCCAAAATATATTTTTCCTTATTTAAAGCATTATATAAATCTTCAGATTTTTTAATAAATTTAAATGTTGTTGAAGCACTTCTTTTTGGTCTTGCGTTTCAGTGGATTGTTAAGTAGTATCGATTATTTCCAAGAGGGAAGAATTTTAAAAGAATTTTTTCTTTAGTCATTTTAATGTTTAGAAGTTTTAAACCTTGAGCATTTTGATGTGTTCGATAGCCTTTAGAACTCATCTTGCAGTAAATATTAAATCTTTTATCATTATTGTCGTTTTGTGAAAGATATTCAGTCAAGTTTTTTTGGCTCTCTTCATCTAAGTAAGAAAGAAAGACATCAAAGTCGACATATTGATTAGCTGAAAGACCTTGAAGACGTGAGTCCATGTTTAAAGATCCACTTAAGCTTTTAGAACTCAAGCGAATTATTTTTTTTTGTCCAAAGTCTAATTTAAAGATTGCTAAACCATTTGTTTTGACTTTATGAACATGATTTAAGTAAATAAAAACTATAATAAAGATGTCTATTAAAATAACACATGCTATTATAATTGCTATTCCCAATAATAATTGATCCATAATGCTCCTTTTTACTAAAATAGCTGTTTTATAGGTTTATTTGTTAATCATCTCTATTTTTTAAAAGTCGAATTGAAGCGTGATTTTTAGCTAAAACTTTGATTGTTTTTTGTCCTGTGAATCTAACGCTAATTGTATCGTTTTCAACAGCCACAACTTGGCCTTCGCCAAAGAAGGTGTGGCTAATAATGTCACCTACTATAATTTTTTTGTTCAAAGCTTTTATTTGTTTTTCGTCAACTATTTCATCAAAATCAAGGCCAACAGAACCTGTTTTAAGAATAAAGTTTTTAAGTTCAATTCCCATTTCACGAATAAAACGTGATGGTTTCTTTTCTAAATCTGTACCAATTAAATATCCACGACTATCGCTAATAAATAATCTATTTCTTGCTCTGGTAATTGCCACATAAGCAAGTCTTCTTTCTTCTTCTAAAGCATCATCTGAGTTTGATTTTAATGATTTATAGTTAGGGAAAATTTGATCACTCATTCCCACTAAAAAGATATTGTCAAATTCTAATCCTTTAGCAGAGTGGACTGTCATAAGTGTTACATAATTTGTAAAGTTATCATATTCATCACCTGCTGAAAGTAGTGAAACTGATTCTAAGTATTCTTTAATACTCTTATTTGGATTCTTCTTTTCTCAAGTTACTATTGAGCGAATTAATTCAGCAACATTATCTTTAGCAGTGCCACGAAGTGAGGTTTCTTCTTCTATATGTTCATAGTAGCCAATTTCCTTTAAGAATTTATCTAAAACAACACTAATTGAGTTGCTTTTTAAAGCTTGACGATAAAGGTTGATTTTGTTAAGAAACTTAACAATGTTGTTTTTAACATTTAAAGGTACTGGCAATTCCTTGTAATATTTAATTAAATATGAATAGAGAGTTAATTTTTTATTATTTGCTGCTTCTTGTAATTTTTTAAGAGTTGCTTCTCCGATTTTACGGTTTGGAACATTAATTATTCTATTTAATGAAACATCAGAGCCATCATAGATAACTCTTAAAAATGCTAGAGCATTCTTAACTTCAGTTCTTTGGAAGAATTTTTGACCATTAAAAATCTTGTGGTTAATATTTTCATTAATTAATTCTTCTTCGAAAGGACGCGAATAATAATTTGAACGGTAAAAAATAGCAATATTTTTAAGCTGAATTTTTTGTTTCTTTAATTCATTAATTTTTTGGACCACTCATCTAGCTTCAGCTTCGACACTAAAAGCATGAATAAATTCTATTGGTGTTCCTTGTTCACGATTTGTTATTAAATCTTTACTAAAACGTTTTTTGTTGTGTTTGATTAATTTGTTAGCTGCATCTAAAATTGTTTTAGTTGAGCGGTAATTTGTATTTAAAATAATCGTTTTAGTATTCTTGAAATCCTTATCAAAATCAAGGATCAAATTAACATCAGCTCCTCTTCAACCATAAATAGTTTGGTCAGGATCACCAACAATTGTTAATTGAGCATTTGAAGATAAGTATTTAACAATATCATATTGGATAGTTGAAGTGTCTTGGAATTCATCAACTAAAACATATGAATATTTTTTACTTCAAATTTTTGCCACTTCAGGGAAGTTGTCAAATAATTCCTTAACTTTGATAATTAAGTCATCAAAATCGAGACATTTTTTGCTTGTTAAATATTGATTATATTTAGAATAAATTTTTCCAACGAGTGCATTAGATCTATGCTTTTCTTTAGCATCTAATTCTTTAACAATTTCGTCTTCACTAAAGCCTTGGTTCTTGGCTCATGAGAAAATCTTCATAACATTTTTGTATGAAACATCTTCATTTGAAATGCCTTCAGCTTTATAAAGTTGAGCAATAATTGTCTTCTTATCTGTTTCATCAATAATTTGGAAGTCATTACTTAAATTAACATGAATAGCTTCCTTCCTTAAAATCTTTGAGCTTAAAGAGTGGAAAGTACAAATATCGATTTGATTAGCTTCTTCGCCGACGTATTGTTTAACACGTTCGCTCATTTCGTTCGCAGCTTTATTAGTGAAAGTCACTGCTAAAATTGAACGTGGGCTAATTCCAAGTTCATTAATTAAATAAGCCACCTTCCTAGTTAAAACTCTAGTTTTACCACTTCCAGCTCCAGCGATGATTCTTAAAGGACCATCAAAATACATTAAAGCTTCTTTTTGTTCAGCATTTAATGAATTCAAAATTTCATCTCGTTTGCTCATATAAGTCTCCTCCTATTTTTTAATGTAAATTACAGCTTCATTATTACTTTTGAATGGTAACAGTTTGATTTCATCTAAATAGCTTGATTGTGAGTAATGTTTTAAGTATGTTACTCTGGTGTTTGTTGAACGATCAAAAAGTCCAAGTCTGGTTCTTAAAGCCATTTCCATAATGTAATTAATTAAGATTAAAGTGAATCAAAAGCCAGATAAAAATATGATTAGTTTTGAGGCAATTTGAAAACGATAATTGCCTTCATGTTTTTTAATTATGAATTGACTAAAGTCATTAAATTCATTTGGCATAACTAGTGTTATTGGGATTAAAATTATTAAAAATCAGAAAATTAAAGCAAATGCATTTCTTCTTAAAGGTAATAATTTTAAAGCTAAAGGAGTCATCTTTTTAGCAGGTATTATTTGTACTCTAAAAATCAGCATTCCAATTGTTCTACTACTATAAAACAATGGAATTAGTAAAAATAGCACAAGTAAAATAACTGTTGAAAATATAATAAAAGAATAAAAGTTAATTGCCTTGACAACACCTTTATGGACCATTAAAAAGTAATAATTTAAAATTGCTAAAGTAACAAAAAGTCCGAAGTCAAATAAATTAATTAATAGTCGATATCAAACATTAATATTTTTATGAAATTTCATTTAGAATCCTATAAATCATCCAAAATATCTTCTCATTTTTTAACTTTAGGTAGTATTTTTAAAAAGTTAGCTTCAGGATTATTTTTAATAACATAAATATTAGTATTAAAGAATAGACTATTGTCTAAATGTTCTTTTTCTAAGTATTTGTAAAGCTCATTAACAAAACGATCTGTACGTTTTTCATTGTAATTAATTCTGTCTTTAATAACATCTTTTGATCAGAATCTTAGTCTTCAGGTATTTAATAAAGCACCTAGAAAAATTTGCAGAAAATACATGCGAGCATAAGCTATTTCTTGCTCAAATTTTAAGTTATTATTTTTAACATAGTTTTCAATTGCTTTAAATTGTTCAATAAATGTTTTAGGATTCAATCACATTGATGAAGGAATAAATTCGCGAACAATTCTTTTGTTTGTATAAAGATAACTTTGTGCTTTAAGTAGCATAAAATAGTTTAGTTCGATACAAAACTTACTATCATTAACAACAATAGAACGGTGTTTTAAAAATTGGTTAATTAAAGATTTTTTGAAAACTTTATTAAAGATAAAAGGATATGAATATGCTAAAACTTGATGTTCTTCTTGAATGTTATAAACTTTTTCTTCTTCTAATCTTGGGTTTGGTTTTCATTTAACTGTGCCAACCAATCTTGGACGATATTCTAAAACATCTGGATTAAATTTTTCAATATAGTTTGTTAATTCTTGTGCAAAGTAAGAACGCATTGAGTTGTCACTATTAATAATTGTGGCATAGTTTCCTTTAACTAAGTGAAAAGCACAAACTATATCACTTTGCATAGTTCTCTGACGATTATTAGTAATAAATTTTAAACGTGTGCCAAAGAATGCTAAATATCTTTCAATAATAGAATACATTTTAGCTGAAGGGTTTCTTAAAGCTAAAATTAATTCAAAATCTTGATTATTTTGATCTTTCATACCTTCTAAAATATTTTTTAAACTTTTAGCGCTATTTATTCCTGGTGAAATAATTGATAATTTCATTTTACTCCTTTACATTTTAATATTTCCAGATGTTCTTGGATATGGAATAACATCTCTAATATTGTCAACACCTGTGACATACATTACTAATCTTTCAAAACCAACTCCAAAACCGCTTGAACCACTATCGCCAAAACGACGCAAGTCTAAATATCATTGCAATTCTTCTTGACTGATTCCTACTTCTAAAGCTCTTTGAAGTAATTTGTCATATCTAACTTCTCTTTGGCTTCCACCTATTAATTCGCCAATTCCTGGCACTAATAAGTCAAAAGCTGCTACAGTTTCATTGTCATCATTTTGTTGCATATAGAATGCTTTAAAACTTTTTGGAAAATTAATTACAGCTACTGGTCCATTAAAGATTTTTTCTGAAATATATCTTTCATGTTCAGTTGCTAAATCAAGTCCGAATTCAATATCTTGATTTTCAAATTGGTCTTTAACTTTAGCCAATTCTTTAATTGCGTCGCGATAGTCAATAACACTAAGTTTTGTTTTTAAAAAGTGATTTAATCTTTCTAAAAGACCTTTGTCAACTGTTTCATTTAAAAATTTAAATTCAGCAGGATGTTTTTTAATTGTATTTTTGATAACAGATTTTAACATGTCATCAGCTAATTTGATTGCATCCTTTAATTCATAGAAAGCAACTTCAGGCTCAATCATTCAAAATTCTGCTGCATGTTTTTTGGTATTTGAGTGTTCTGCTCTAAAAGTAGGTGCAAAAGTATAAACTTTTTTAAATCCAATTGCATAACTTTCAGCATGCAATTGTCCTGTAACTCCTAAAGTAGCTTTTCTAGTCCCAAAAAAAGGATCTTTTGAATTTCCATCATTAACTACAAAAGTTTCTCCAGCTCCTTCACCATCATTGCTGGTAATAATAGGAGCATTGAAGTATAAGTAACCGTGTTCATCAAAATATTTATTAACTTCTTTAGCTAACGTTGATCTTATTAACATTGTAGCTCTTAAAAGATTTGTTCTATGTCTAACATGAGGAATATCTCTTAAGGTTTCTAAATTGATTCCTTGTTTTTGAATTGGAAAGTCTAAATCAGCATCTCTTAATAATTTAACTTTAGTAGCGACTAACTCTAAAGGTTGTTGAGCTTTAGGAGTTAAAGCTACTATTCCTTTAACTTCAACTGCAGCACCTAAATGAATTTGATCTAATTTGTCAAAGTCAAATTTTTCGCCTTTGCAAGTTACTTGTAATGATTGAACAGTTGAACCATCATTAATACTAATAAATCTAATTTTTTTGTTACCTCTATTTGATGTAACTCATGCTTTAATTTCTAAAGTTTTGTTTTTAAATTCTTTACTCTTCTCAAATAAACTTTTTATGTCACTTGTTGCCATATCCACCTCTAATTAATACTAAATTTATTTATTATTTATTAATAATAATTTTAGCATAGAATATTAATATTAATATTAAATGAGTTTAAAAAACTTCAAAAAGTTGGTTCTGATAACAAAAAAAATTTGCAAAATAATTTAATTTAAAAATTTATCTATATAATTGCATAGGTTTATATTTAAAAAATAATAATTATTTTTTGGCCAATAATACTGTTTTATAGGCTAATAAGCAATTTTAAGTACATTATTAATTTATATTTTATTTAATTTATGAAGGGACAAAAATATGGAAATAGCTCAATATGTTTTTGGAACTTTGGCTAGTTTATTAATGGTTTCAATCTGCATACCTCAAATTATCCAAACTCTAAAAACCAAAAGTGTTGGAAATGTTGCATATTCAACATTCATTATTTACTTTTTTGGTGGATTTATTTTTGTGCTTACTATGTTATTAAAAGATGGGGTTGGAACTTATGGTTTAAGCGATCCCCTTGTTAATATAATTGGTAATGTTACATTTGCAATTTTAATGGCCTTTACTATTACTTTATTCTTTATATATGATAAAAAAACTAATAAAGTTTTTAAAATTGGTATTGGTTCATTACTTTGAATTTTAGCTTTAAGTGGTTTAGTATTTTTCATTGTAGTATATGCAAACAAAAATGCTAGAACTAACTTAGGACCTGACAACGGTTGAATGATAGCGATGTCTGTTATTGCAACTTGTTGTTGTGCTTTACCTTTTACAATTCAAATTGTGAAAACAATTAAAAGTAAAAGCGCGGATGGTCTTAGCTTACCAATGCTTTATTTAGGAATTGTATTAAATGCATTATTATGTATTTATTTAGGACTAGTAGTTCCATTTAATACAGCTACTTGATATGTTTACGTAATTTTCCAATTAGTAGCAATAGCTGTTTATGTTGTACAAACTTACTTATATTATCATTTCAAAAATAAAGCTAAAAACGTTCAAAGTGAACAAGAAGTTCAAATTGAAACTAATAACTAAAATTCAAAATGATTATGAGGCTTTTAAGCCTCATTTTTATTTTTAAAAAATTAATTTAGTATAAAATAAAAAAGATTAACGTAATAAAAAAATGGAGGCAATTATGCAAATTAATAAAAAAGAAATTAATAATTTATATAAAAAATATTTAAAAAATTCAAACAATAAATTAATAGAAAGCGCTGTTACTAAAAATGGTATTTTCAATGCAAGTTATAACAATGACATGCAAACAAAACATAATTTTGAATTTAATACAGAAGTAGCTAAAGCTGGAATGACTAATCAAAAAAGTTCAGGACGTTGCTGAATTTTTGCAGGTCTAAATATGCTTAAATTAAAAGCTACTAAAAGTCTAAATGTTGAAAATTTCGAATTTAGCCAAAATTACTTTTTATTTTGAGACAAGTTAGAAAAATCAAATACATTTTTACAATTAATGATTGATAATCCTAAATTAAAATATGAAGATCGTCTTTTTGCTTTCTTAATGGAAGGCAGTGTTAGCGATGGTGGATATTGAGAATGAGTATCAGGCTTAATTAAAAAATATGGAGCTGTTCCTAAAATCGTTATGCCTGAAACATTTAACTCAAGCAATACTCACGGTTTAAATACTGCATTAAATTGTCAATTAATTAGTGCGACAAAAGAAATTAGAGAAGCTATTAAAAATAAAGCTAGTCAAGAAGTTTTAATGAAAATTAAACATGAAGCTCTTGAAAGAATTTTTGAAATCAATGCTAAATCATTAGGTTTACCTCCTCAAAAATTTTCATTTGAATATCGTGATAAATATAAAAAATTTATCAGAATCGAAAATGTAACACCATTAGAATTCTTAGAAAAACATGTAGGTACAGATTTTCTTAATAAAGTTAATTTATTAGATGATCCTCGTAAAGAAAATCCTAAAAATAGATTGCTTGTAGCTAAATACTACAAATCAGTTATCGAAGAAAAAGGATTAGAGTCACTTAATGTTACAATGGATGTAATTAAAAAAGCTGTTATTGCTTCATTAAAAGATAATGAGCCAGTTTGATTTGATTGTGATGTTGATGCTTATAGCGATCGTAAAGGTGGAATATTTGATATTGATCTTTATGAATATGAAGAAATGTTGAATATTAAAAATACTTTAACTAAAGAAGATAGAGTTAATTATCGTCTTTCAGTTCAAACTCATGCTATGACAATTGTTGGTGTTAATTTAGATAAAGATAATAATCCAATTAATTGAGAAATTGAAAACTCATGAGGAGAAGATGTTGGTAAAAAAGGTTACTTCTCAATGAGTGATAGATGATTTGATGAATATGTTTTTGGAGTTATTGTAGATCCTAAATATGTAGATAAAGTTGCTCTTAAAGGTTTAGAAAAACCTGCTATTGAATTAGAGCCTTGAGATCCTTTATCATAATAAATTATTAGTATAAAAAAATAACCAATAAGGCTGTTTTATAGTTTAATTGGTTATTTTTTTAAGTTTGTCTTTTTTAAGTTTAATTGCTAGATAAATAGTAATTGAAATAAGTATTATGACTGCTAAAGTTATTAAAACAATAGCTCAAGTTTTAGTTGTATTATCACTATTATTTATCTGTTCACTTGCTATTTTTAACATGCAAGCAGGACACTGACAATTAGTGGCATGTATAATCATTCTAGCCTCCTAAATTAAAACCATTAAAAAGGCAAAAGCAAATCCAAAAATTAATAATGAAGAGATAATATCAGTAAATGTATTAATTAATGAAGCTGATATTGAACTTGGATCTTTTTTAATTTTAGCTCAAATTATTGGAAATGCAGCACCTAGTAATTTTGAAATTGTTAAAGCTAATCATAAAGCAATTGAAGAACCTATTATTATTGCTCAATAAATCATTTTGTTAGTTGACATTAAATCTCTTGTAGCTGCAAAATAAATGCCTAAACGTGCAAAGTTAATTACAGCTAATATTAAACCTGTGACAAAACCGATTAAGGTTTCTTTTCAAATAACTTTACCATATTCACCAGGTTCTATTTCTTTTAAAGCTAAGGCTCGATTGACTGAAATATTAGCTTGAATTCCGCTATTACTTGTAGCATTATTAATTACTGGTATAAGGGCTGCAAAAGAAATTGTTGCTAATGAAATTTCGGCTACATCTTTAATTTTTTTAGCAATAGTTCATGTTCCAAAATACACTATAATTTGGGTCACTGTGGCAAAAAGAAGTAGTAATATTAATCATAAAATTCTTGATTTAATTATTTTATATCATGGAGTTTTTAAGTATTCACTTTCATTAACTTCATGGCTACTAATACCTGCCATTTTGTATAAATCTTCTGTAGCTTCTTCTTTAATAACATCAATAACGTCGTCACTTGTAATCATTCCAACTAATCTTTTTTCATGGTTAACTACAGGTAATACTGACATATCGTGTTCACTAAAAACTTGGGCTGCTACTTCGGTTTTATCATGAGCTGAAACATAAGTTACAGGATTATAAATATCATCAATTTTAGCATTAGGATCAGCAAAAACTATTTCTTCTAGTGTTAAAGCACCAATTAGTTTATTTGTTTCATTAACTACGTAATAGTAATGCACTAATTCAGCATTTTTCTTATGATAATCACGTCTAATTTTATGTAAAGCTTGTTCGCAAGTATATGAATTACCAATTGAAGAAATGTCTATTGACATTATGCTTCCAACTTCATCATCTTCATAGCTTAAAAGTTTATTTAATTCAGCTCTTTTTTCTTGAGGTGTATAAGCTAAGATTTTGCTTGTGACATTCGAAGGTAATTCTTCTAAAACATCAGCTAGTTCATCACTTTGTAATTCTTGTAGTAGTTTCATACCTCAATCTTCTGTAAAACTTTGAGCTAAATTAGCTTGGACTTCTTCATCAAGGTAGCTAAATAATTCAGCAGCTTCACTAGTTTTAAGTAAAGGCAAAAAAGTTAGCTGTTCTTCTAACTGTAATTCATTTAATAAGTTTGCAACATCAGCCATTGGATTATTATCAATAAATTCTCTGGCTTTAGTTACATTTTTAGTTTTAATAATTTCATGTAGTTCGTTTTGCAAACTAATCTCTTGACTTTCGCTCATTTTAATCCTTTAAGTATTTAACTAGTTGTTCTTGGAATTGTTCAAGACGAATGTTGTAGAAAGTACCATTTTTAACAAATTTAACATCCCCTGTTTCTTCACTAACAATTATTGTGGTAGCATCACAAAGTTCACTAATTCCCATAGCAGCTCTATGACGAGCACCATATTGATTATCAATTGATTTTCTGGTGATTTTATAAAAAGTAGAAGCATAATAAATTTTATTATCTCTTATAATTACAGCTCCATCATGCAATGGACTATATTTATTGAATATTGAAATAAGTAAGCTACTACTGATATTAGCATTTAAAATTACCCCATCAGTTCTCAAATTGTCAATATTATCATTGTTTTCAATTGTAATTAAAGCTCCAATTTTATTTTTTGATAAATATTCAACAGCTTCACGTAGTTGGTTAATTAATCTAATTTGACTACTTTGACCTAATTTTTGGAAATGACTTCTAGCCATTTTGTTTTTAATTGCATTAACTATAAGTGGCATTATTGAAATTAAAATTAAAGTAACTAATAATGCAACAATTACATAAAAACCGATTTTAGATTCTAAACACATATTTTGCTCCTAAGCATAAACTCTTGAAATTAAAACAGCTAGTAGTGTGCAAAGTAAAATAATTAAAACTGATAAGCTAGCTCCTAAAACTTTAAGAATAATTTTTTGCTTTCTAACTCTAGGATCTTCTTTCTCATGTTTATTTACTAAACACATAAATTCTTGGCATTCACCAGTAATAGGTTTTTCATCTTCAATCAAAGCTGGATTAGCTAATTCAGCTTGATTTTCATTATAAAGTTTTAATAGTAATTCTCTTCTTTTTTGAATTCTTTCAGCACATGTTGCTAGATCTCTATCAATAATTGCCATATACACCTCTTTCAATTGATATTAAATTTAATACTAATAATTAATATTTTATATTATATTCCATTAAAGTGAAATATATTAGTTAGGAAGTAACTAATTTTATTCAACAGCTTCAATAATTAATTTAACTTTTGGTCATATTTTTAATTTAGTGGCATATTTTTATAACAAATTATAATTAGGTTTATTATTTTTATATTTTTTGATAATTTTGTTATTTTAAAAGTGAGACACTAAATTTTAGAGATCTCACTTTTAAAATAATAGAGAACAGCTATTTTTCATTAACAATATTTAATTTTTTTAATTTCAAATTTCTTTTATTTGTTATTTTCTTAGTAAATAATTTAAAATGTTTTTTAGCTTTGACAAAAATTGTTTTTAGATAAAAGAATTTAAAATATGTTTTTTCGCTATCAAATAATTTTTTGTTAATTTCGTCTATCTGTTTTTTGATATTCTTTATATATAATTGATTTTCTTCTGAATTTTCTTTTATAACGATAGAAATTGTGTAAATAAAATTATTTTTAAAATTCGATAATTTTTCAATTAAATATGAATTTGCATCAAAATGATTTTTTTCATAAAAAGGATCGATTTTTAATTTTTCTTTTTTTCTTTCATTTAAATACAAATCTTTTTTAAAAAATATTCTTACAAATGAAACTGTAAGATAAACAAAAGTTATTCAAAATAAAATTACTCATTTATTAAATGTTTTAAGAACTGGAATTTCATTATTTAAAAAAAGTGCTAAAATAATAGGCAATAAGAACAAAGCAAAATTTCAAAAACTATCTAAATAATAAAATGTCAATTTCAATTTTGTTTTTTCAATATTATTTATTTTTGTTCAAAACCAAATTTTTTTATTATATTTCGCGCCTAATATCTCATGTAAATCAATATTTGATTCATCATTTTTTTCACAATAAGCATCAAAAAAACTAAAAAATTCTATATCTTCACAACTATTCCTAATAAAATCTATAATTTTAAATTTGTTTTGTAATTTAATATTAAAATGTTCATTTTTCCATTGAAAAATAATAAATAAATTGTTTTTATTTTCTACCAAAATTTTTTTTACTATACTAATATCCAAAATAATTCTCGGAAATTCTTTAAATATAAAAAAATTCTTTTTTTGGAAACTTGTATATGCACTCTTTAATGTTTTTCATATTTTTCAATTGCAATTATTTTTATTGTCTGCAATTTTTATTGTTAATTCGTTGTTTTCTAAATTATTTTTTGATTCTAAATATAATTGATTGTCTTGTTTTAAAAAACTAAATTTTATTATTCAAAAAACTAAATTTAATATAAGTAAAACACTTAATATAAGTATAGCAATTGTTCTTAAAAAAATGTCATTCAAATAAATAAATACATTTCAAATGAGAACGGAAAAGATGAGCAAATTAGAAACTAATAGTAAAAATATATAAATTTTGAAGAAAAGATTGTAATTAATGTGAATTTTTGAATCTTTTTTAAGTGTAAAGATGATAGTTAGAATATATCTTGTAATAATAATTATTAACGATAATATTAACGATAATATTAAAGAAATTAAAATATATTTAGTATTAATCTCGCTTAAATTTTTCATAGTCTAATTATAAACTAATTGAATAAGTCGCCTTTACTTTTTATATAATTTGCACAAAATATCTATAAAAAAGGCTAATTGATAAATTATAAGTTGTAACTTATTATGGTTTAGTTGCCATTTTTTCTTGAGCAGTTTCTTTCAATTAAGTTTTTATTTTTAAGTTGATTAATAATTTTTTTAATATAGCCTTCACTTAAAGATAAAGCACTGCTTAATTGGTTTATTGTTATATTTGATTCTTTTTCTATAGTTTTAATTACTTTAATTTGATTATCATTTAAATCCTTTTTATTTTCATTTTTATTTATTCTTTTAAACTCTTTACGATTAAAAGGAATTTTTACTCAAATTGTATTTTCATCAATTTCAAATGCTTCTTCGTCATATTTTTTGACAATTAAAGGCACTCCATATCCAGTTTGTTCAACTAGATCTAATTGCATAAAAATTTTTTGTAATTTTTCATTTACTGGTCTACTTACGCCTTTGAAAAATTCCTCTTTAGTCATATTTCTAGAAATTCCACCATTTGATTCTATTTGAATATAGTCTTTGAATATATTAACTTGAGGTGGATATTTTTCATCTCAATGATTATGAATGCATGCATTTAGTCATGCTTCTCTAAAACACTCAAAATCGAATAATTTTTGTTTACTCCTGGTTGCAGTTGACAAATCTACATAAGTATCATTAATAGAACCTACATAATTTAAAATGCTCTCAATTGCATAAAATAAACATTTATTTCCATATTCGTTTCTTTTCAACAAAACACTTTTATCATTATCTTTAAAAGTATTAACTTTAATTGAAATATCATTATTATTTGATAATAAATAAGACATTAAATTGTATTTATTATTTTTAGTCTTTAAATCAAAATTTTTTTCGAAAGTATCTTTATTTACTGTTAAATTTTTAATATTAGCATATAGCAAAAAACTATTAAATGTCAAATCTTGATCTTTGTTTTCAATTTCACAAATATTTATTTTAAAAACTGATTTTTGCACAAAATCTACTAATTTATCTCTATTAAGTTTTTTATCTTCTTCTCCAATACGAATATAATATTTATCATAAGCTGAATAAGGTTTTTGTTCTCCTTCAGCTTCAACTTTAATTACATTTTGGTTATCAATAAATTCAATCGAAATTCTAGGCATTATTTCTGGCTTAACATTGTTTGAAATAGCTTGAGAAATATCTCTCATGGATTTATTACAAATATCTAAGTCAACAATTGTATGATCATCTTTTAGACTAAAATAAATTGTTCCCGTTCCTCTTTTATTGAGCATTGCGGTTAAAGAAATAATCGATTCTTTTTAATTCACAAGTTGATTTTTTGTATTCAACTTCTTCACTCTCTCATGGCATTTTATTCATGTTTATCTCCTTATAATTTAATTAAAAGCTATTTTTTATTTATCACTTTATTTGCTACTTTATTTATCACTCTTTTAAAAATTTGAAATAAAAATTTAAAAACTAAACTTAAAATTGATTCTTTATTACAAAAATAATTAAATATAATATTTAAAAAAATTTATAATAAAAGATATCATTAATTTAACTAAAGATAGAAGGGAGTCAAGATGCTTAAAATATTTAAATATATGCCAGCTAGAATTAAGTTTTTTGCTTTTCTAAGTGCCTTTTTTGCTATCTTACAACCTTTTTTATCAATGCTTAATCCTACTATTACAAAACAATTAATTACTTTGATAGCTAAAGTTCAAAATGGTACTAGTATGAATGAAGTAAGTTTACTATTTTGAAATATAACAACTAGTGGTACTAGTCAAAGCATGATCTATTTAATAATTATGGCCATAGGTTTTGCATTTTTGTTAATGATTGTTGCTTTTCTTAGTGCATTCTTTGCTGCTAGAACTTGTATTTATGGAACTTATTATTTAAGAAAAAATTTGTTTGAGCATCTTTTAACTCTTTCAAGAAGCAATTTAGAGAAATATTCAGTTGGTAGCTTAATTACTCGTTTTAGTAATGACTTAAACAAAATTAAAGATGTGATATTTGTAGTTGTTAGAGGGCTAGTTGTTTCACCTTTCTTTGTAGTTTGAGGACTTGTTTTTACAACCACAACTAATGTTTATTTATCAATAGCTATTTACATTATTATTCCTTTTTTAGTAGGAGCAGCAGTTTTTGCAATTTTTAAATTATTTCCTTACTATCGTAAAGAAAACTGAATTTTAGATCAACTTAATGAATTATCAAAAGAAGATATTAATGCTATTGCTTTAATTAAATCATATAACCTTGAAGAACCTCGTTATGAAAAATACTTTAATAAAAATCAGGAATGATTTACTATTTATCGTAAAAATGTTGTTTTTGGTTCATTTGCTTGACCAATAATTAATGGATTTGTGCTTTTTGGCAATGTAGCTATTTTTGCCCTAGTAGCCTATTTAATAGGCTCTAAAGGCGATGACAATGTTGCAAAATTAATTGGTGATGTATCACAATTTACAGCGAATTTAAGCATGATTTCAATGGGCGTTTTTAACACACTTTTTACAATTAATAGACTATTTAGATCAAGTGTTTCAGCTAAAAGATATATTGAATTAATGAATTCAAAAAGTGAAATTCCTTTTGTAGTTAGTGATAAAAAAGTAACTAGTGGGGCTATTGAATTTAAGAATGTTAATTTTGCCTATCCTATCTATAAAAAAGGCAAATTGGTCGATACTAAAGAAGCTTTAAAGAATATAAATTTAAAAATTGAAGCAGGCTAAAAAATTGGAATTATAGGTAAAACTGGAAGTGGCAAAAGTACACTTGTTAAACTTTTAACTCATGAATATTTACTAGATAAAAAAGATGGCGAAATCATTATTGACTCACACAATATTAATGAAATAGATACCAATAACTTTTATGAAAAAGTTACTCAAGTTTATCAAAAACCAATGATTATTTCTGGCTCAATTAAAGAAAATATTACTTTTTCAAATCATGAATTAAGTGAACAAGAAATTGAAAAAGCTATTAATATTTCATGTGCCAACTTTATTAAAGAATTAGATGACAAATATGAAGCTAGAGTTGATCAACGTGGTAAAAACTTTAGTGGTGGTCAACGTCAAAGAGTTGCTATTGCTCAAGCTTTAATTAAAAAACCTCAAATTTTAATTTTAGATGATTCAACTAGTGCTCTAGATAATGAAACTGATTTAAGAGTACGTCAAAACATTAAAGAAAACTTAAAAGATAGCACTCTAATAGTAATTAGTCAAAGAATCAATTCAATTAAAGATTCAAATCAAATCTTTGTTCTTGATAAAGGACAAATTGTTGCTAGCGGTAAACACAGTCAACTATTAAACACAAGTTCTATATATAAAGATATATGACAATCTCAAGAACAAAAAGTAGGTGAATAATGCCTCGATTTAGTAATTTAAGATTTAGACAAAAAGGTGAAAAGAAAATTAGCTTTAAAATTTTCTTCAAATTTTTAAGCAATTTTCAAATTTCAAAAAGTGTTTGAATTTTTACAGTGCTTTTTGGTCTTTTAGTAGCTAGCTTAACTAATATTGCTTCTTGATTAATGGGTTTTATTACTGATAAATTTTTAGATCCTAAAACTTTCAATTTAGCTAATTTTAAAATGAAAGACTATATTGTCTTTGTTGTAATGTTAGCTTTAGTTTATGTCTTTATAAAAATTTTTGAAATTTCGCAAAGAGTTATTGTTTCTAAGCTTAGTGCTCGCCTTGGTCGGAATTTAAGGCTGCAATGCTATCAAAAAATTCAACAAATGGAAATGAGTTTCTATGAAGAAGAACAAACTGGTGACTTAATGTCAGCATTAACTAATGATATTCAAAATATAACTGAAGCAATCTGAGACATTTTAATTTCAGTTATATCAATAATATTTGAATTTAGCTTTATTACAATTATGATGTTTATTTATGTTCCACTTTTAGCAGCTATTATTTAGCAATTGTGCCAATTAATGCTTCAGTAATTTTCTTAATTATTAGAAGTAACCAAAAATTCTACATCCAACAACAAGAAAGACTCGGTGAATTTAATGGTTATTTAGAAGAAATAATGGATGCTTTTCCTTTAGTTAACTTGCACCAACAAAAAGAAAATGTCGCTAAAGAATTTGACAAATATTCTCGTGCATTAATTAAACCTGATGCTAGTGCTACTACTAGAATGAATATTGTTTTCCCTTGATTCCATTTTAGTAAAACTATTAATTTAATTATTGTAATTGCTGCTAGTGCTGCCTTTGTTTACTACAAAGTTCCAACAGGTGGCATTAAACCTTTGAGTGCTGGTGTGGTTATGTCATTTACTCTTTATTTATTTAGAATCAGCGACAATATTTCGTTATTGCTTGAAGTAATTAATGGTATTCAGCATGGGCTTGGATCAATAGTTAGAATTAATAAAATATTAGCTTTAGTGCCTCAATTTGATGAAGTTAAATTAAACGAATTAGACTATAAAACAGGCAAAATCGAATTTAAGAACGTTTCTTTTGCTTATGCTTCTAAACCAGATAAGTTAGTCTTAAAAAACATTAGTTTCACTATTGAAAAAGGTAAAAGCTTAGCTTTAGTTGGCCACACTGGTTGTGGTAAAACAACTATAGCTAAACTACTTTCAAAATTCTATGTACCAACTAGTGGCGACATCTTAATTGATGGCCAAAGTATTCTTAAAACAAAAAGTAAAAGTTGAAGAAACTATATTGATACTATTTTACAAGAAACTTATATTTTTAATGATACAGTCAAAAATAATTTAACTGTTGTTAATCCAAATATAAGCGATAAAGACTTTAATGACATAGTTGAAAAAACTTCAGTAGCCGAATTCATTAATTTAATGCCTGAAAAATTTGATACAGTTTTAAAATCAAATGGTGCTAATTTAAGTGAAGGGCAAAAACAATTAATTGCTATTAGCCGTTCATTAATTGCTTCTAAACCAATTTCAATTTTAGATGAAGCTACTTCTAATATTGACACAATAACAGAATTAAAAGTGCAACATGCAATGAAATACTTAATGAAAGATCGAAGTATGTTAATTATTGCTCACCGTTTAAGTACTATTAAAAATGTTGATGAAATTCTAGTTATTAATGAAGGTGAAATAATTGAACGTGGTAATCATGACCAATTAATGAAACAAAATGGACACTACAAAAAAATCTATTCTCTTGGTTTAAATGATACAAAAATTGATTAGTAAAAATAGTCAGCTGCAATACTTTTGCAGCTGATTTTAATTTTTATTTAGAATATCTAAATCAAGTAAAAATTGTTTGATTCCATAAATTTTATAACCATTAGTATCAGTATCAAAAATAGGGTCATCTTTAGTAATAATAATTTTTTTAAAATTATCATTGATGTTTTTAAAAGGTCTTTTTTCTTGAATTTCTTTATTTTGATCATACATATTTAAACAAGATTGAATATAAACTTTTTGATTACCTTTTTGAGCAATAAAATCTACTTCTAATTGAACTTTCTGATTAGCATTAGGTCTTATTTCTACTATTCCAACATTAACATTGTAACCTCTTAAAAGCAATTCATTATAAATGATATTTTCCATTAAATGATTTTCTTCATTTTGTGCAAAATTGATTTTAGCATTTCTTAAACCTATATCTTCAAAATAATATTTGTATGAAGCATTAATATACTTTTTACCTTTAATATCATATTTTTTGACTTCATTAATTATGAAAGCATTTTTTAAATTTTCAATATATTTTCTAATTGTTGCATCAGAAATGTTTGTTTTCTTAATTGATTTAAAAGTTAATGATAATTTATAAGGATTAGTGTATGAACCTATATTAGAAGCTAAAATATTGATTATGTCATCTAAGTTATCAATATCTTCTATTTTATTTTTTTCAACTATATCTATTAAATAAGTTCTTTTGAACTCATTTACCAAATAATTAGTTTTAATGTTGGCTTCTTTCATGTTTTGAATTAATGGCAAACCTCCATAAATTAAATATGATTTTCATAACTCATCAAGGTTTTTAAATTCTTTGTGTGAGGCTATTTCATAGAAAGATAAGGGAAAAACATGAAGCGAAATCGATCTACCTCTAAATTCTGTAACTACATCATTAATTAAGAATTTAGAATTGGAGCCAGTGACAAAAACATTAAGTTGTTGATTATTATTAAGTTGATTTAAAACAGGCACAAAATCTTTTAAAAGTTGCACTTCATCAATTAAAAGAAAATACTTTTTTCGATCTTTAATTTTACTTTTTATAAATTCCAAAAAATTATTAGGATCACAATAAGAATGATTTTCTTGATTTTCTAAATTAATTTCAATAATATGATTTTGATCAACTTTTAACTCATTTATTAAATATTCCTTGAATAAATTAAATAACAAAAAAGACTTTCCACTTCTTCTTAAGCCTGAAATTATTTTAATAAGAGGTATATCAATCATCTCTTTAAGTTTTTCTAAATATATATCCCGTTTAATTACCATAATTGCCTTTCATAATGAAACTTTGACACTTTTGTAAAAGTTTCTTCCTACATATATTTTACATTATTATGTAACAAAAAAGAAGATTATAAAATTAATTTTTTACACTTTTGTAAAAGTTTCATTATGAAAAAAATAAAAATCAAAACTAGGCTATTAACCCAGTCTCAATATATGGTGCCCGAGACAGGACTTGAACCTGCAAGCCGTTAAGCACTAGATCCTTAGTCTAGCGTGTTTGCCAATTTCACCACTCGGGCATAATAAGTAAATTTATTTTACCAAAAGATTAACTTTTTAGTTAAATAAATTTTTACTTTTTTTATATTTCTTTTAAATCTTTAAATAGCTTGGCTATTCCACTATTTTCAAAAGAAGGCCCTATATAATGTGCTACTTCTTTTACCTCATCCATTGCATTTTCCATAGCAATGAATGTTCCTATCTCAGGTAAAGCATTTAAATCATTTCCGCTATCACCAATGTGAGCAGCGTTTTTAGGATCTATTTTTAATAAGTCACAAACAAATGCTGCACCTTTACCTTTGGTTGCATTTTTATGTGTGATTTCAATTGAGTAACCTTTTGAAACTATATGAAATGCAAGTGAAGGATATTTTTTAGCTAAAAAGTTCTTAAAATTTTTAGTAGCTATTTCATCCTTACCAAAAGTTAAAATTTTAGTTACATTAGTTTTTTGACCTAAATCACTATATTTCAATTTTTCTAATTTAGCAGTTCAAGGGCGCATGTAGGCACTTGATTCATAGTTACAATAGATTTTATTTTCGCCATTAAAAATAAAAAACATTTCTTCTTCTTTTAAAAGCTTAACTATTTCATCAACTTGATTTTGTTCCATCTCATATCTAGCCAAAACTTTATTTTTCTTATCAACTATTAAAGCTCCATTTTGACAAATGGCATAAGGGCACTTTAACAATTTAACTAAGGATAAAACAAATTCGCTATTACTTCTACCTGTTGAAATAATAACTGGTTTAGTTTTATTTAATTTAACAACAGCTTTAACATTAGCACTTGAAAGAGTTTCTTCACCTTCTGGTAAGTCTAATAATGTTCCATCTAAATCTATAAAATAAGCTTGAATTTCTTTTTTCATAATTTTAATTATATATAAAGATTTTATATATGAAACAAAAAAAATATATCTATAATTTCCAAGCTTTCAGTCATTGACTAATAATGCTGTTTTATAGATATATTGGTAAAATATGTTCATTTAAAAATTAATTTTTTGCTTAATTAAACTATTTTAATTTTCTTTATTTTTAGTTTAATCTTCACCATCATAATCACTATTTTTATTTTCGACTTCTGTTATAGCAGCAGCATAGCAATAGAATCAATCTGCATTTCTATTAAATATTTTTGAAAAATCATTCATTTTTTCTAAATATATATTTTGAACGTCTTTTCAAAGATCGCACTCTTTACATTCTTCTACTCTTTTCATTAAAGAATTTGTATTAGGAACATTATATCAAAATCATCTTTCTTTTTTGGCATCTGATTTTTTAAAATGTGCGAGTTGAATTCAATATTCTGGTCCAAATTCAAAAACAATATTTAAATTAATTATAAAAGGAGTATCTTTATAATCATCAATTGTTAGATTTTCAGCTGTTAATGAAAGAGAATGATCTTTTGATATTTCGCAGCCAAGCTGAGTTAGTGATTCATCAAAATTCTTTTTTACAAATTCTCTTATCTTTTTACAGTCATATAAACTGTTGCCTTCAAAAAAAGCATAAACAATAAGGTTATAATCTATTATAATTACGCCTTTATCATTTTGAACTATTAATTCTTCGCTTCCACTGCCAAATAGTTCTTTTTGAACTCTAATATATTTTTGTTTGTTGATTCTTTTGATCAAATCTTTAATCAAAGTTGAACCAAATGATTCAATGTTTTTAATTAAAGATTTATTTTTTACAAAACTGTACAAATTTACCTCCTATACTTATTAATATTATCTAATTAAATTTTATTAAATTTCTTTTTCTTTTTTTATAACTTTATTAACTTGACAAATTGTTAAGAATTTTAAATAAGACAATAATGCAATAGTTAAACCTAAAACATAATAAGATTCAACCTTAAATGAATTAACTAAATCGCTAGTTTTAGAATCACTTATTTCTTTAGTTTTATTTAGATAAGGTAATACTTTTGTTATAGAAGTATAAATATCACTAAATAACTCAAATTTGGGATTATTGAAAATAAAACATGGAACAAATTGTTCATTTCAATACTCGTGACAATAATCTTTAATGTTGATACATTTATTTAAAGCATCTTCTTTACTATACGCTAATGATATAAAGTTTGATAAAGTACAATAAAGTGCTTTATAAAGATAAATATAAAATTCTAAAAGCTTTATAGTTTCACTGTCATTACTTGAATAAAAAGGTTGAAGATCTTTAACAAAAATTTTATTAATTTTTAAATCTTGAAATTTAGCTAAAAATTCATTATATTTTTCTTTTGCCAACTTTTGGAAACTATTATTTGAAACAGTTTCAAAATTGTAATTTTCAGTATCTTGATAATCAACATATTTATTTTTGAATACTAAAATATCCACTTTATTTAATTTGTCATTTAATGCTTTAGCAATTGAGTCTTGTAAAGACTGAATAAATTTATAAAATGAATGCTTAATTAAATAATCAATTGCTTCACTATCTTCATTGTTGTCTTTACTATTCTTATTCTTTTCATATTTGTTAGCATTATTAAATCAGATTTTAAATGCTTCTAAATTAGTAGCATTATTTAAACCTTTAGCGAAAAATAGATAATAATCTACATGTCAAATTCAAAATGAAAATTGATTATCTTCTAATCACATTAGGTTTCTAAATCATTCTTCTTTGCCTAATTCTTTTTCAGCTTCTTTTTCACTTTCATATTTAAGTCTAAAAGCATAATATTTATTATCATAACGAGGCAAGAAATAAATTCTTATTTTCTCATTAGTATTAGTAACTTTCTCTTTGATATATTTTTTTAATAATTCATAATAATATTCATCAAAAGCAGTCCAATCATCTAAACGAATTTTCTTTTCTTGATCTTGACTATAATCATATAAATATTCATTAAATTGCATTTGTGTAATTGTTATATCTAATTTCATAATCTCCTTTATTAGCTATTTAATTATATAAAAGGAGTATTCAATAATGTATATTATTGGAAATTGTGCAATAAAAACATATCCTAGATATTCTAAAATATGCTAAAAATATTATTTTGTATTTTTAAGTGCATCTTTTTTAAGACTATTGATTGTTAAGTATGAGAAGTATTCTTGATCTAAACTTCATATTCAAAAGCAGAATTATTAATACTATTTTGATTTAAATAATCTAATAAAATTAAGTTAATGCTTGCTACTAGTAATAAGTAACCATAGACTATTTCAAATTTTGCATTCTTAGAAGAAGTTATTACTTGATATTTTCTAATTAAGCTCTTATAACCTTGGCCATCAACAATGTAATAAGGGAGAAAATCGATATTAACATTTTTGAACATTTTGTAATTCAAAATATCTCAAATATTTTTATTTTTTAATTCTTTAGTGAGTTTATTGTCATTGTCATATAAATTGGTAATTATTTTTTCTTTAAAAATAAAATTAGTAAATTCATTATTCAAAGAAGTTGTTTCTCCATTTATAACAAATAATTTTAATTCTTCTTTTTGGCTTTTTAGTCTATAAACAGCAATCTTGTTTACATTAGGAAATTTAGTTGTTAAATAGTCTCTAACTTTTAGTATTTGTCTTCTTTATAGACTTTTCCGTCATTTTTACCTATATTTTCATTTATCTCTTTTATGGTTATATTTATGTAAAATATTATTTCCGAAAGTGAGGGTGGTAAGTTTTAAAAATAAGATAATGTTTTTTAGGCATTATCTTATTTTGTTCTACAATATTATTAGGTATTAATGTAAAGTCAATTCCTTTCTGTTGAGATAAAAAGAGACTTTACACAAAATTCTAAACAATTTCTTTGAATTATTTTATTAAGATTGGCATTCTTCAATTATTGGCAATTCAGTAAGCCTTTTACCATTAAATTGGAAATATTTAGGTCCTTGATAAGCTCCACTACTATTAACTTTACCTTTTCTAGTATAAATTTCTCTTGTTAATCCAGACAATGATCAACTTTGATTTTCAAAATATACAGTTTTATTTGATTCAACAATTGCTTTTATGTTTGAATTTTCTATGAATGAAACAACATCTCCATCTTTTAAACCTCTACTATAAAAGTCAAATGGTTCACCTCTTTTTGTTTTTTCTTTAGTATGTACTTCGACATTATCACCCATTAATTCTAATTTAGCTTCATTTCCTAATAATTCCTTAATATCAAGTAAAATGTCATAAGCTTTTTCAGGTGGTATATTAAAAAATTCTCTATTTTTGTTTATTCTTAATTTTGAAATTCGATCAATACTTTTGTGAATCATTTTTTCAACTTCATTATATTTTTCAGTTTGTAAAGTGGCATAGATTTCATAAGGAAGTGGAACAGCTGTATTATATAATTCACGACCTCTAACTTCAGGCATTCTACTGCTTTTACCAATTTTAACCCAATCATCTCTAAAACTTGGGTTAGTTAAAACATAAACATATCCTTTTTCTGGCATTTTATCTCCTTTGTAACATTATCAATAATTTTACAACTTAAGGTGGGGTTTAAAAAGTATTTACTTCATCCAAAGAATTATAAAACTATTATATATATCTTTAAATATGAATAATCTAGTATTTGTAACTAGAAGTAAGAAGTATTTGTAAATTTAGCTTTTCATTTCAATATCAAGTTTTGTTAAAACGATAGCGCTTTAGGCTAATACCATAATCATTATTATGAGAAGATAGGCAAAAATATTAAAGATATTACTGAAGAATTGCCTTTTGAAATACCAGAGAATTGAGCTTGAGTTAGACATAACAACATTTTTGAAATAATTGGTGGTTCTCAACCTCCTAAGAGCAAATTTATAGAACATGAAAAACAAGGATATATTAGACTTTATCAAATACGTGATTATGGTGAAAATCCAAATCCAGTTTACATACCCTCAAAATTTGCTTTTAAACAATCAGAAAAAAATGATATTTTGTTGGCCAGATATGGAGCATCAATTGGTAAGGTATTTTTTGCAGAAAATGGCGCTTATAATGTAGCTTTAGCAAAAGTTAAAAAAATGTTTATTAATGATTGAATAAACAAAGAGTTTATGTTTATTTTTTATAAATCAAGTATTTATCAAACATTAGTTAAAAATAATTCAAGAAGTGCGCAAGCCGGATTTAATAAAGACGATTTAAAAAATCTTTTTATGCCAATTCCTTCTTTAAATGAATCGTCAAGAATTGTAAGTAAATGAAACGATTTAAATAAATTAATTAATGAATATGAAAATAAAGAAAATCAACTATTTAAACTTGATTCCAAAATTAAAGATAAATTGCAAAAATCTATTTTGCAATATGCAATTCAGGGTAAATTAGTTAAACAAGATCCCAATGACGAACCAGCTTCTAAATTATTAGAAGCTATACAAATTGAAAAAAATGAGCTAATTAAAGAAGGAAAAATAAAGAAAGATAAACAAGAATCTTTCATTTTTCAAGGTGAAGATAAAAATTATTATGAGAAGATTGGTTCGAAAGTAATAAATATTACAAATGAAATACCTTTCGAAATACCAAAAAAATGGGCTTGGGTTAGACAAAAAAACATTTTAAAATTGACAAAAAATGAAGCTTCTAAAAATGGAAATTACCCTTATCTAGAAGCAAAGGTACTAAGAAAAATTATTAAACCGAAAATTATTAATAATGGGGTTTTAATAAATAAAGGAGATATTGTTATTTTGGTTGATGGTGAGAATTCAGGTGAAACATTTGTTCTAGACCAAACTGGTTATATGGGTAGTACATTTAAACTATTAAAAATAAATAACAAAATAGATCAGGAATATGTTTTAATGTTATTAAAATTTTATAAAGAATTATTCAAGAAAAATAAAAAAGGAGCTGCAATACCACATTTAAATATAGACATTTTCAATAATTTACTTTTGGCTATTCCAAACATAAAAGAACAAAAAGAAATCATTTTAAAACTTAAAAAGATCGATAATTTTATTAGTTATTAATAAAATATTATTTTGATTTTTGATAGGGGGTATAGGAATTAACATTTTTTGCAATTTTTCCTTATTTAATGTTTTTCCTTTGATAGCATTAATTTTTTCACAATTATTGATCAATAATCCTAAAAATATTAATAGAAACATTTTTGTTATATTATTATCTTTGTAAAAATTTATTGAAACAATTGCTTCATTATGAACTGAATCTTGATTAATTATTGATGTTTTACCTATTGTTAATTTAAAACTCAATAATAAAGTTCCTTTTTTGGACATTTGATTATTAAATATTTCTTTTTTTGCTTGGAGAGATATTTTTTTCTTAGAATCTTCAATAATTTGTTTATCTTTCATATCTGCAATTGTGAATCAATTTATTACACCATTTTTTCAATAATTAATATTATTTGTTTCTGGTGATTTACCTAATACCAAATTTGCGATATTTTTAAAACGAGTTCAAGCTCAATTTATTGGTATTTCGAAAGGTATTTCATTTGTAATATTTATTACTTTCGAACCAATCTTCTCATAATAATTTTTATCTTCACCTTGAAAAATGAAAGATTCATGTTTATCTTTCTTTATTTTTCCTTCTTTAATTAGTTTGTTTTTTTCAATTTGTATAGCTTCTAATAATTTTGATGCTGGTTCATCATTAGGATCTTGTTTAACTAATTTTCCTTGAATTGCATATTGTAAGATAGATTTTTGTAATTTGTCTTTAATTTCAGAATCTAGTTTGAATAATTGATTTTCTTTTTCTAAATATTTCTTCATAAATTTATCTAATGATATTAATTTGTTAATGATTTTTTCTTGTTCATTTATTGGCGGAATAGCAATTAAAATATTTAGCAATTTTTCATCATTAATTGATTTTTGAGTTACTCCACTGCAAAAAGAATCTTTTAAAATTTGATAATAAGGAGACTCTATAAACAGTTTTAAATATTTTATTTATATAAATAGATAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTCACGAGGTAGTAAAAAATAAAGGATTAATTTTTAAAGGTTTCAAAAAAACAGTACCATCGCCTCCTGGAACGAAGTTAAAATCTACAAATTTTGTTGTTTTTGTGTGATCTCCAAAGATTATAATCGGTTCTTTAACTTTAAAAATATTATTTACATTATTTGTATATCCTTCAATAAATTTTTGGCCTTGTGTAATTATAGGATATTTGCCTGTTGATAATATTTGTTTAGTTTTTATTATTATATTTTTTTTATCTATCCTAAAAGAAATGTTACTTATTTTAACAATGACTCAATTTTTTGGTATTTCAAAAGGTATTTCGTTTGTTATATTTATAACTTTTGAGCCAATCTTCTCATAATAATTTTTATCTTCACCTTGAAAAATTAAAGATTCTTGTTTATCTTTCTTTATTTTTCCTTCTTTAATTAGCTTATTTTTTTCAATTTGTATAGCTTCTAAAAGCTTAGAAGCTGGTTCATCATTAGGATCTTGTTTAACCAATTTTCCCTGAATTGCATATTGCAAAATAGATTTTTGCAATTTATCTTTAATTTCGGAATCAAGTTTAAATAGTTGATTTTCTGTTTTTTTATATAACACCTAAATAGTATTCTAATAAATAGTCAAGACCTGGTATTAGCCAGGTTTTTTTGTTCAAATAGGTGAATGAGTTTACTTTTCAAAATACTTTTATTTTAGGAAAATTCTTTTATATTTAAACTTAACTTAAAAATTATTTATTATCTTACTTCATATTGTATGTGGTTTATATAAATGTATTATACATTTATATCAAACCACCACACCAACTAAGGAAATCAAATCAATAATTTTTTCCTATGCTTTAACCTATACACCGAATTTTCCTAAAGAAACAGGGAATATATAGTGATATACTTCCCAAAATTAAAATTTCAGGACTATATATACCTCCCCCTTTGAAAAAAAAGACCCACCGGAGTTTTTAATGCCCTTGTTATTTTAGAAAGGAAAAATATATGCTACTCACTACAAAACAGTTACAGGAATTTTACTAAATTGAAATGGAAAATATTGCAAAAAATAAGTCTCTTATAACCAACTTCCGTCACATTGATAATTATGCAATGGTTTTAAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTTGCGTTTGAGTCTTATACAATAATAAGTTTTATTTTTTGTATTTTCAAAAATTATCAAAGGATGAATTTTAAATTTTGTATCGATATTTAGATGAAAAATATCTTCTAATAAAGGATTTGTAATTTTTAATGGTCTTCATAGTTCTAAACTCATATAAAAATTATACAAAAAAGAGGATTAAACCTCTTTTTAAGACGAACTAGAGTCTAAGGCGTATCATCAAGCGCTTAAAGATAAAATATGAGCTGCTTGCTTGTTCGATTACTCATATAACAAAATCCTTCTTAGACTATTGTTTAAATTATACTACAAATAAATAAAAAAATTAATAATTTTTCAAATCTTATTTGCATTGTTTACTCTTAAACAAATTGATAATCTTTTACTAAAAAATTATAAAAGGGTGGGCTTGAATTTTTCCGTTATTTAAAACTTTGCCATCAATTGCGGTATATACCATATTTAAATAATCTTCAAATACTTGCACTTGTACTGGAGTTCTTAAATTGTCAAATTTTCATTCGTTAGCTTTGTAAGACTTAACACATAGCTTAATGTTTTTTAATCTTATTTTCATTTCTCTTACTATGAAATATAATAGGAAATTTGATTTAAATAATTCCATCATATTGCTAATTCAAGTAAGATAGTATTTTATTTTTCTTCTTATTTCTTTATATCAAAACTCTATAAATTGTATATGAGTCATTAAATGACTGCAGCCATGTTTAGAAGAATTGACACAACTCATTAATTTGTTAATATCATAACTTAATGGTAAGTCTGACATATCACTAACCAAAGCTCACAAATCATTTAAATCTTTGTCACTACAATTTTTTAATGTTTCTCTTGAAATTAACATTTTTATTTTCCTTTCTTTATTTTTTAATATATAATAAATTTGCGGTTGTTCGGTTCAGGTTTAAGAGCAATCGCTCTTTTTTATTTAATTAAACTGAACAACAATGATAAGTAAAACAAAAGGTGAATGGCAAAATCCACTCACCTTTCCTTATTATTAAATATGAAATAATAAATCTAGTGATTAGCAAAAGGTTCAGGATTTTTACTTATCACAATTATTATATCGGGAATATATAACAATGTCAAATAAATTTATGAAAATTTTTCACGTTATAATTTAACTATGAAAATGCCAAGCTATAAAGATCTTAATTTTAATATCAAGAGAGAATTAAATAAATTAAAACCTAAAAGCTTTGAAGAATGAAAAGAAGCTAAATCAAAAGTTTTTTGCATTGCTCTTGGTAAATATTTAACCAAAATTAATAGCCACCAACCTGATATATTTTTTATTTTGCAAATAATTTATTAATTTCTTCATCGCTAAGAACAACCGGCTTAGCCTTAATTCTGTCTTTTTTATAGATTTGTATTTCTTTGAATTTCTTAATTGCAACTTGTTTCTTGCTCTTGCCAGCTTTAAAGCCTTTTTTATTCAAGTATCATCTAATACCATATATGCCACCAACAGCAAGACCTAAGGCAAGCATAACCGATACAATTCAAATTCAATAATTACTTTTATGGCTTGCTTTTGTTTTATCAACATCTAAATCTTCACCATTTGGCTTTTTATGCTTATCGTTTTGTATTTCTTTAAATCATAATTTATCATCAAAAGCATTATAATTTGCCTCAATATTATTCACCACTATAGCATAGGCAAAATTAATATTATTGCCTTCTTTAACTGGTAATAAAACAAATACTTGCCTCTAGCTTTATAATCTAAGCTTTTATAAAAGTTATCGTTTTGTATTTCTTTAATTGCCTTTTCTAAGCTTCGTGGTNACTTCTAATAGCTCAGTATCTTTATTTGATACGGAATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTATTATAGCGGGAATATATAAGAATGTCAAATAAATTTATGAAAATTTTTCACGTTATAATTTAACTATGAAAATGCCAAACTATAAAGATCTTAATTTTAATATCAAGAGAGAATTAAATAAATTAAAACCTAAAAGCTTTGAAGAATGAAAAGAAGCTAAATCAAAAGTTTTTTGCCTTGCCCTTGGTAAATATTTAACCAAAATTAATAGCCACCAACCTGATATATTTAAAAATTATTATCAAGTTCTTTTTTATCATTCATTTATTAATTATAAAAGAGAAGAAGAATTATTGTCTATTCTAAAAGCTAAAGGTTACCAAGCTAAGAGAACAAATCTTAAAATGGATGGCATATATAAAATTGATATAGTGGCAACAAAAGGAAATGAGAAGATTTATATTCAAGTTAAGTTAAACAAACCAAAAGCTTTAGAATTGAAGAATTTGCATTCATTCTTAAGATCTAAGGCCTTTCAAGGACTAATTGCATGAAAAGAGTCAACTGAGTGAAAATTCAAAGAAATTTAACATTTTTTAACATATTTAAAATTTTGATATAAAATAAAGTTGAAAAGTGAATTGTTTTATTCACTAAAAATCTTAAATACCAGATATTGACGGGAATATATAGTGGGAATATATAAAGGAATATATTTTTAAACATTTGATAGAACAAAAACCTTTTGAAAATAATATGCTGCAAAGTGTTTTTAATCATATGATGAATTTTTAGTTATTGGTGGCCTTCCTTATATTATTGATAAATTTTAGTTAATCCCAATACAAAAAGTAATTTTGGAAAAGTTTTTGAATATCAAAAAGAATTACAAAATATTTATAAAGATGATTTTGGACAATATTTAGATTATGAAGAAATGATGAAAGTTGAAAGAATTTATGATATGGTTGGCAAATTCTTAGGACGTGAAAACAAGAAATTTGTTTTTAATGATCTTAATTTGAATTTAAAAAGCAAACGCTGTATACCTTCTATAGACTGATTAAAACATATTGGAGTTATTAATTACTGTTATCAATTAAAAAATATTGAAAAGCCAATTAAAACAAACTTTATTGAAAACTCATTTAAAATATATCACTTTGACACAGGTATGCTTATTTCACAATTAGATGTTGAAAGTATGAATGATTTAAGAATCAATAAAAACTTCAATACATATAATGGCGCCTTGTATGAAAATGCAATAGCTAATAGTTTATACAAACAGGACTTTAAATTATTTTATTGAAAGAACAACAAAGGTATAGCTAAAGTAGATTTTGTTGTTGAAACTTTTAACAATGTTATTCCTATTGAAGTAAAAGCTAATAAAGGAGATAGTTCTTCTTTAAATAAATTTTTAAGTGAGTTTAGAAATAAAAATGTTGCTTTTGGTATTAAACTTTCAAGAAACAATATAAGTTTTCAAAATGACATTTATACCATTCCATATTTCTTAGCTTTTAAATTAAAAGAGTTTATAAAACATATTGAAAAAAATTTATAAATTTTCTTTTTTAATATATTAAAAAAGAGTTTTTAGAAGTAAAAAATCTGTTTTTCAAATCTACTTTTTGCCTTTATAATGATACATATAGCAAGAAGGAGATTTAGCCATGAAATCATATTTATTAAGACAAAAATATACTCAAGAAGAATGAGATGCCTATAATAGAGAAGAGGCTAAAAGAATTGCTAAAAGGGATTATTTTAGACTTCAATATGTTAAAGGCGCTGATAAAAAATTAGACAAACAATTAGATAGTGCCATTATTAAAGTAGACAAATTTGAACATTTATTTAAGTAGAAATTTTACCGAAATAACTGTTTTATAGATATATATAAAAATTGCAGCTCTTTATATGAGTTGCAATTTGTTTTATTTTTTGTCTTCTTTTTTCTTTAGTTCACCTTTGATGTGGCAGACTCTACAACGAGCTTCATAAGTATCATCACCAAGCGAATTTAATGATTGTTCGTTTGTTTTTCTAAAACTAAAACCTGCACTAGATTTACATTTCATGCATACCGCTTTTAGCTTTGTAATGTGATCTGCTATTGCTAAGATTGCAGGAGTTACACCAAAAGGTCTTCTTAAGAAATCCATATCAAGTCCCGAGCAAAGAACCCTAACTCCTTTTAAAACTAATTCATCAATAACATCAAGAATGCCTTCATCTAAAAAGTTAATTTCATCAATAGCAACAGCTTTATAAGAAGGGTCTCAAAATTTCATTATATCACTTGATTTTTTAATATTTTTAGCTATAACTTTTGCACCTGTTCTACTTACTATTTCTGTTTCAGAAAAGCGAGTATCAAATTTAGGTTTAAGAACTAAAGTTTTAATATCTGCTATTTCTAAAATTTTAATTCTTTTAATAAGCTCTTCTGTTTTACCTGAGAACATTGGGCCAGTTATAACTTCTAAAATCCCATCACTTTGTTTTAAATACATTGTTTTTTCCTCCAAGTTAGTAAATAAAAAGTCCAATAAGTAATTTAATAGATATGGACTTTATTATTCAAATTAAATTAAAGCAAGCTCTGTAAGTGTTTTGACTAAAACACCTTGTGGCACTTCATTAATGTCTGCTGTACCTGCGACATCACAGTGGATGTATTCAACGCCTTCTGTAAATTCTTTTAAGAACATAGCAGCTGAGCTTGATCCACCATAACCGCTTAAGTCTGTGTTTTTAAGATCAGCAACTTTAGAAGCTTTGATTCCTTTTGAGAATGCTTCATCAAATGGCATTCTTCATACTAATTCATGCGCATTGTCTGCAGCTTTAGAAATATCTTCTCAAGCTTTTTCGCTTGTTGCTCAAATACCTGTGTAGGTTTGACCTAAAGCAATAAGAATAGCACCAGTTAATGTAGCAACGTCAATTAAACGTGTTGCATGAAGTTTGGTTGCTCCATAAACTAAACCATCAGCCATAACTAAACGACCCTCAGCATCTGTGTTGTTGATTTCAACGCTTTTACCATTCATTGCTTTTCAAACTGAGTCAGGTAATGAAGCATCACCATTAACTCTGTTGTCTGTAATACACATAATTGCTGAAACATTTTTCTTAGGTTTAAGTTGTGCGATAGCTTTTAATGTTGAAGCAACAATAACACTACCTGACATGTCGAATTTCATACCTAACATGTTTCTTGGACTCTTAAGTGAGTAACCACCTGAGTCAAAAGTAATACCTTTACCGACTAAAACTGTTTTTTCTTTACTTGAAGGATCACCAGCATATTCGATAACAACTACACGTGGTTCATAAACTGACCCTCTGTTAACTGAAAGTAATAATCCCATTTTAAGGTCTTGAATTTGTTTTTTATTTAAAACAGTAACTTTTAAGTTTTTGTATTGTTTGAAATCTTTAACAATTTGGTCTGCTAAATATTCTGAGTTGCAAATGTTTGGTGGTGTAACTTGTAAGTTACGAGCATAATTTGTAGCTTCAGCTAAAATTAATGTTTTTTCTAATCTTTCTTCAACTTCTTTAGTTACTTTTTCTACATAAGGAACTAAAGTAAATTCTTTAGCATCTTTATCTTTAGCTTTTTCATTGTATAAAGAAGCTTTAACAAAGTAAACTGCTCTTGTAAAAGCTTCAACAACATCAGCAACTGAAACATTTTTTGTTGCAAATGAAGCTAAATCAACACAATAGTTACGTGCGCTATTTTCACTTAAAGATCTAAAAAATCTTTCAAGAGTTCTAAAATCTAACTTGCTTTCTTCTCCTAAGTAAACATAAGCTTTGTTTTCACTAAGATATTCTGTAATTTCGTTGTTTTTTTCGATTAAATATTCAATCTTTTTAGCATCTTTAAAAGTTGCTTGCAATAAAACATCTTTAGTAGCTTTATTTACTAATTTTGTAAAAAGTGACATAATTAACCTCCTATTATTTAATTTAATTATTTATATTTTACAATATATTAAAAAGGCAGTATTAAAAGAGTACAAAAATTTTGGTTGCCTATGTTAATGGTTACATAAAAAAACTTATTCCTTTTTTGGAATAAGTAAAAATTTAAAATTAAATTAGAATGAATTTCTTTCCAAAAAGACTGGAATTTAAAGCTCACTCTAGAGTCTTTTAAAACATTTCTTCATTTTCCAATTTCTTTCTAAAGAAATCTGTATCATGAATGAAAATCAAATTTTCAATGATATTTTCTAGTCTTTCAACTACACGATAATATGAGTGCAATTCAAAAGAAAACTTAATAATTATTTCGTTAGAATACTCATCATTTTTGGTTAATTTACGAGCCATTTTTTTGATCTCTTTATCACAGAATTGTTTGTATTCTACTTTTTGTTTTTGGAATTCTTTGAAGAATTCTTTAGTACTTTTTTCATCTTGAAGACGGCTACTATATTCTTTAAAAATTTTGCTTAATTCTATATTCATAGCTAAAGCAGCATTAACAACCTCTTGAGGAATTTTTTTGTTTTGATAGAAGAATCTGGCCATAAAATAGAGGTAATCACAAATTCTCTCAATGTCTCCTGAAGAAGTTAAAACACAAATATATCAACGCAAGTGGCTTAATAAAGGTTGGTCTTTTGAAATGTCTCAAAGCAATTCTTCAATGGTATTTACTTGAGTTAAGTCGCTTTGTTTTTCACGATTATTGATTTCTAAAAATAAGCCTTCAATATCAATTTTGTTTTGATATATTGAAATAAGTTCAGTTAATTTAAGATGCATTTGATATGCTTGGTCTAAGTAATCAAAAAACATTTTTCTTAAGTTATCTTCGCTCTTCTTTAATATAGTGTAATTTATTGGCATAATTCTATCCTATCTTTCCCATAATGTAGTCTTTTGTTTCTTCTTTAAGTGGTTTAGTAAATATGTTTCTAGTGCTATTAATTTCAATTAATTCACCTGCTAAAAAGAATGCAGTTTTATCACTAACTCTCTGAGCTTGAGCCATTGAGTGAGTTACTAAAATAATGGTGTAATCATTTTTTAAATCTAAAATTAAGTTCTCTATTTTTTGAGTTGCAATTGGATCTAGCGCACTAGTAGGTTCATCCATTAAAAGTACTTTTGGACTTAGCGCTATTGCTCGAGCAATAGCTAATCTTTGCTTTTGACCACCGCTTAATGCTTCTGCATTTTCTTTAATTTTATCTTTAACTTCATCATAAAGTGCAGCTCTTTCTAAAGCTTCTTGCACTTTTTGGTCTAATATTTTTTTATCTCTAATTCCAATATTTCTTAGTCCAATAGCAACATTATCATAAATAGAAAGAGGGAAAACACTTGGCTGTTGAAATATCATTCCGACTTGCTTTCTTAATTCAATATCATTAATAAATTTTTTGCTAAAGAGATCTTGATTTAAGTATTCAACTTTACCTTCTAGATAATTTCCAGCTTGTGGCCCATGAATTCGATTCAAAAGTTTAATAGTTGTACTCTTGCCACAACCACTAGGCCCAATTAAAGCAACTGTTTCGTTTTCTTTGAATTCTAGATTGATATTTTTTAGAATTTGTTTACCTTTTTTGTTGTATCAAAAGTTAACACTTTCTAATTTAAAAATTGTATTTTTTTCCATATTTACTCCTAATGTTTTCTTTTTAATTTGCTTAATTTTGGAATCACAAATTCTGATAATGAAAAGAGGATAACTATTGTCACAAATGACATCATAGCACAGTAGTACATAGTTGGAATTGAGTAAGCAGAAGGTAATGATAATTGAGCATTCATTCTAGTTGTTAATGTTTGGCCAAATAATAATAAGCTAATATGTTTTGAATTACTTAGTCCGCTAGTTAAAATAAATGGTGTTGTTTCTGAAAAGATTTTAGCCAGCATTAAAATAATAATATTTAAAAGATTAGGCCAAATTTTAGGTAATTTTATTTTGAATAAAATTGAACTTTCGCTGAGTCCTAAAGCAGCTGCACTAGTTACTACACTTTGATCAACTATTTCTAGAGCTTCTTTAATTCAGCGACAGCTGAAGGCAAATAACATAACGCTAATTGCTAAAGCTCCAGCTAAAAGTGAGTTACTGGTTTTACCACCTAAAGCCAATTGAAATGTTCCTAAAAAGATAGCTGAACCAAAGACACCAAAAAGTAAAGCAGGCATTGAAGTCATAACTGAAATGAAGTTATCAAATACTAATTTTGCTTTTTTGCTTTTGTTAAATTCAACAAAATAAATTGTTACTAAAAAAGCAAAAGGGAAGGTTATTAAAATTGCTACTAAAGAGATATATAAAGTGTTAAATAAAGCTCTAAAGTTGCCCACATTTGTTTCAGCATCAATTTTGCCTAAAGCTAAATAATTTAAGACTCGATAAAAAAGATCAATAGCAAATCACGAGTAAAATATTAAGACAAAAGTTAAAGCAACAATTTCCCAAAACCACTTGTTTATAGTATATAAAATATTTATAGTTTTATAATTTTTATCTTTTTTCAACAGGAAATCTTTGATTTCTTGTCATGTTTTAAACTTGTCTAATCACTTAGGTTTTTTGACTTTTAAATTAACAATAATAATGATTGCAGCATTAATTAAAGTTATCACAATTAATAGAACAAAAGCTAAATTAAACATGTAACTTCTAGTTGCATCGCCACCATTTTCAGCAAAGAAATTAGCATTAATAAATACTGAAAGAGGACGCAATGAAGAGTTAAAAAATTTCTTAATATTTGTAAAATTTTCTGAATAATTATTGCTATTTAAAATAAACATTAAAGCAGCTGATTCACCGATAACTCTGGCAAAAGAGCTAATATAAATTAAAAATATTTTTTTGTTTAATGACTTTTTAATTATTTTATAAATTACTTTTGATTCACTTAAACCAATAGCACTACCTGAGAGTTTTAGACATAAATCAACTTTTTCAAATTGCAATAATAATGAATTGATCATAGTTGGAATGCACATTAAACTTAAGACCATTGTGGCATTAAATAAGTTTGTCCCATTTTCCATATTAAATATATTTTTGTTCATTCAAGTCAAAGGCACTAAACCAAAAAGCCCATATAAAACAGAAGGCAAAGCACTTAAAATTAAAGCTATAAATTTAAATATTCGATAAGCTTTATAGTTTTTATTTCGATAAATTATTAAGATTGCAACCCTCAAAGAAATAGGACAACTAATAAATAAAACCAATATGCAAATTATCAAACTAGTTAATAATGGGAAATATAAACCACCTTTAACTCCTGCGATTTTATAATCAAAATAAAAGATTTTTACACCAAATTCTTTTCAGCTTAATAATGATTTAAAGAAGATAAAAGCAACAAAGGTAACTAAAACAGCAAGAGCTAAAAGGCTCATCACTAATGAAATATATTTTAAAGATTTATTTTTATTTTCATTCTTCAGTGTTTTTTGCATGTCTCACCAATTAACTTTATGTTAACAAAAACAAGAAAAAATAAAATAAAAAAAGAAAAATATTTATAATAATGACATGAAAAAAAAGAAAAATCTTTTGCGAGCACTTGCCCCAATTTATTCCCTACCCTTAGTTACTTTTACAACCTCTTGTAACTTTTCAGTTATTAGTTTCAAGGGTTCAAGTAGCATGATTCAGTATTTAAATGATATTTCTAAAGAATTAGGTTATGAATATGAAATGAATATTGTAGGAGGAGGCAGTGGCACTGGCCTAAGTCAATTAATTCATGGCAAAACTGATTTTGCTTCAATAAGTAAAGATCCACGTTTTGGTTTGAATTCGTCAAAGAAAAAACAAGCTAAAAAAGAATATTTAGAAAATCAACTTAAGACTTTTTTAGTTGCAAAAGAAAATGTTGCTTTTGTACTTAAACTTCCTAAATCAAAGATTCAATTATCAAGTGGAGAAGTTGAAGCAAAAGATTTAAATGTTTATTTTAACAAAAATAATTGAAGAATATTATTAGAATCTTTTGGTGGTCAAGAACAATTGACTTTAGCTGATATTACTTTAAATAATGATTCTAAATTAAAAGAATTTAACATTACTCCTTTTTATAAATCAGGAGGAGCAAACTTCAGCGGAACAGCTGAAGCTTTATCAAAACAAAATCCTTTTCTTGATATTAAACCAAGTGAAAAAGCAGCTAAATTTTTAGAAGATACAAGTGATAAACCAAAGTATGATGTCAAATTAGAAAATGATTCAAGTAATGAATTTTGAATGTCAATGAAGCATTATGATAAACCATGTTTAGGCTTTTTTACCTTAGATTTTATTAAGAAAAATATAGAAGAGTTCAAACGATATAATTGCTTAGTTTTAAGTTATTTGAATCCTGATAATAACAAATATTATAGTCCTTTAAATGATCGTGAATTATTTGATTATGTATGAAATAGAGGTTTCTATTTAGTCATAGGTTTAAAGAAAAATTATAAAAAAGAATTGAAGTTTTTATATACAATATTTGATCCAAAAAACAAAGCAAAAATCCAAAAAGTTTATTCAAAAAATGCATTAACTTTTCCAACAAATGATGAAATAATTACAAATTTTAATTTAAGAGACATGTCAGAATTTAAATGACCAGAAATCATGGATAAAGTCAAACCAGATACAAAATTTGATTAACAAAAAATTGCTAATTTTATTTTTAAAATTTTACTTAAAATACTATAAAACAGTTGAATTAGTTCAAAATAGTATTTATTTTTTCAAACTAAAACGTAACATTTTTATTATTTCCAAAATCGAGGGTGGTAAGTTTAAAAAAACACATTTTTCTAAATCGCCTGCTTAAATTTTAAATTAAAAAATCATTGAAGGTCGTATTGTTTATTCATAATAATTTAAGGTGAAAAATTATTTGCACTCATTGCATATGGATATTCTTTAATTTTATGTTTTATGAATGAGTTAGTTTCATTTTGAATTCATGCAATATATTTGTTTGATAAATTGTCTAATGTTTTCCTTTTGGAATATATTTTCTAATAAATTTATTGCAGTTTTCATCTAGATTCATTTTATCTATTATTGTTTAAATTGATTTTTAAAATAGTATTTTTCTTCTTGATTTCATTGCTTTCTTTTTTGCAATTATATTCATTTATAAAGTTTTATGTTGCTTAATAAATGTTTTTATTTATTTATAATATTTTCGCTACAAAAACAATTTCTTTTATATCTGTAAGAATTGAGATTTTTAATTTTGCTTTCTAAAGAAATAATCTTTACTTTATGACCCTACTCATTACACTTTGCACATCTCAAAAGTTTGTTTTTTATCTCTTCTGGAATGTCTAAAATCCGAAGCACAGTTGTCAATAAATTTACGTATAGCATTGTACTAAAATTAACAAATTTTGAAATATTTTGTATTGAAGTTCTAGCTCTTAAATGATTCAAAATATCTTTAGAAGTATCCAAATTAATTTGATTTTTGTGAACCTTATTTCTAAAATAATTGTTAGAAATTCATTCGCTATCTGGATCATCTAAAATGATTAAATGAATGTTTTTTGTGTAATCTTTAATGCAATTTATATTATAAAGGACAATTTAAAAAGTGAGATCTCTAAAACCTAGGGGTCTCACTTTTGGGATTTACAAAAATGTAACATTTTTAAAAAATAATTAATATATATTAAAATTAATATAAAATATTAAAGCATATATTAAATAGCTAATAGCTTCAAAAAGGACAATATATGAGAAAAAGAAAAATAAATATTAAAGAAATTATTAAGGAATTCGAACTAACAATTTGTAATGAAGAATGTGATCCTATTTACCGAGATGTTCAAGAACCTGCGATTAAAAGATTAGGTTTAGAATTATCAGGTCAAATTGAAAACACTCGTTATTCAAGAAATATTATTTGTTGAGGTACGAGTGAATCAATTTTCTTTACAAAAAAAGGTAAAGAAGAAGCTATCAACATTTTAGCTCGAGTCCTTAAAGAAGAACCGCCTTTAGTTATTCTTTCAAAAGGGGTTAGAGAGTTACCTAAAAAATGAATTTTAAAGGTTTGCAACAAATATAGAATACCGGTTTTCTCAACTGAAAACTCATCTACATCAATCATTTCAGCTTCTATTGGAACATATTTAAGTGAAAAATATTCCGAAGAAGTTCAAGTTCATGGTTGTTTAGTTTTAATCGGTGGTGTTGGAGTTTTAATTGTTGGACCAAGTGGTTCAGGTAAAAGTGAAGCAACCCTAGAATTAATTCAGAGAGGTCATATTTTTGTTAGTGATGATGCTGTTTTAGTAAAGCTTGTTGGTAATACCTATTATGGTAAAAGTCCTGCTATTACTAAAAACTTTTTGGAAGCTAGAGGTATTGGTTTGATTGATATGAAATATACTTATGGAGTTAAGTCAGTAACTGATAATTGTGAAATTGATTTAGTTGTTGAATTAGTAAGTAACCAAACAGAAGAAGCAAAAAATTTAGATCGCTTAGGTCTTTCAGATCTTAAGTTTCCAATTTTAGATGGAGCTTTACCTAAGATTGAAATTCCAGTTAAAGATGGTTCAAGTGCTGCTAGCTTAATCGAAGCAGCCGTAAGTACTTTCTTAGCAAGAAAAGATGGAATCGATGTTTTAAAACAAATACAAGAGAGAGGAGAAAAAGAACATGAATTATAAATATGAATTATCGCCAATTCCAGCGAATACTCATACACCATTATTTCATATTGGTAATTGACCAATTCAAGTTTATTCATTAACTATTATGCTAGGTATGTTGGCTTCTATTTTGACAATTGTTATTTTTTGACAAAGAGAAAAATATAAATGAGAAATTCTTTTAACTCTTATTTTAATTACAATTCCTTCCGCTATTATTGGTGCCCGTCTTTGAGACTTAGTCCAAGAAGCAATTTATAATCCAAACTATAACTGGAAGAATTGATACAAAATCTGAGAAGGTGGTTTAAGTATCCAAGGTGGTGTTGTCTTGGCCTTTGTACTTGACATGATTTATGTTTATACCAAAAGAGATCTAGTTGACATTCGTAAAGTTGCTTCAATTATTATTCCAACAATTCTAATTGGTCAAGTTATTGGACGTTGAGGTAATTACGCTAACCATGAGGTTTATGGAAAAATAGATCCTACTGGAGCAAGTTGTGAAATTTGAGGAAGAAATTTTGCCCAAAACATGTTTATTAGCGATGATATTTCAAAAGGCGCTTATCGTTATCCTCTCTTCTTATATGAATCTCTTGCTAACTTAGTTGGTTACATTGTTTTAGTATGAATTATTAACCAATTTGGTTTATTAAAACCAGGTTCAAATGCTTCATTATACTTTGTGTGATATGGTCTTGTCCGTTTAGCAATGGAACCATTGCGTGAAGACCAATACCACATGTATACAATAACCTCTATTGTATTTATTGGTTTAGGTGGTTTAGCTTTTGTCTTCTTTGAATTCTTCAATCCAACTCACTATGTTCGTGTTTGAAGAAAGGGCAGATTCCAATATGGTTATGCTCATCCTGAAAAATATATCCAATGAATTGAAAAAACAAGAATTTTTAAAACTAAAAAAGTAAGCCAATAACAGAGTTAGTGCAATAATGGACAAAAAAGATATTAAAAAATTTGATGTTTTAATTGTTGGATCAGGACCCGCAGGACTAAATGCTGCTCTTTATACCTCTCGAGCAGGACTAAATACAGCTTTTATTGATAATGATGTCTTTGGTGGCAAAATTCAAAAAACAGCTAAGGTAGATAACTACCTTGGAATTCAAGATATAAGTGGTTTTGACTTATCACAAAAATTTTACGAACATGCCTTAAAATATGGCGCAAAACATCTTTATGGCAAGGTTGTAAAAATCAATTCAATTAATGAAAATTTACAAGAAGCAATTCTTGAAAATGGTGAAATTATTCAAGCTAAAAAATTAATTATAGCTTCAGGTATGATTAATAGATATCCTAAAGGTGTTGATAATTATCAAAGACTTGAAGAATATGTAAGCTTTTGTGCTATTTGCGATGGTGCATTATATAAGAATAGAAAAGTAGTTGTATTAGGCGGCGGAAATTCTGCAGTTGAAGAAGGAACTTTTTTAGCTAAATTATGTACTGATGTAACAATAGTTACAAATCAAAATACATTTAGTGCTGAGGAAGAATTGGTTGAGGAACTCAAAAAGCAAAAAAATGCTAAAATTTATTATGATCATGAATTAATTTCTTTAAATGGTGAAAATCAGTTAACTTCAGTAACTATAAGGAATAAAAAAACCAATAAAGAAATTAATATTGTAGCTGATGGCTTATTTGTTTTCATAGGCTTTATACCAAACCAAGATGCATTTAAGCATCTAAAAATAACAACTAAAGAAGGTTTTGTAAAAACCAATGAAGATATGGAAACAAAAGTAAAAGGCATCTATGCAATTGGTGATATCAGAGATAAAAAAGTAAGACAAATTATTACAGCAGCTGCTGATGGTGCAATAGCTGCAAAACACATTTCTGATACATTAAAAAATTAGAAAGGGGACAATATGTTTAGAACTTTAAAAGAAAGATTTATAACATTAACAGTAATGTTTGCTCTTGCTTCATTAATGACTATTATAGCTATGGCATTATTTGGAGTTTCAAGAATTGTATTCACTACTTTAGGTGGTGTTACATCCTTAGGAATGTCAATAGCAGCATTTATCTTAGCAATTATAGCCTTTGTCTTTGTTCTTATTATTTACTTGCTTTACAATAGAATAATGGCAAAACGTACAGAAGATTTTAGTCGTTTTAGACACACAAAAGCATCACAAATTTTTGTTTTAGTAGCTTTAATTTGTTATGCTGTTGCAGCAGTTACAGCAATTTTGTATTTTGTAATGGTAGCTATTGTTCATAATAATAACTCAAGTACAATTAGTTGAGAAACAGTAAGAGCATTTTCAATTGTTTTTGAAATTTTCTTCTGAATTGGAATTGTAATTGGCTGATTGTTTATAATTCTTTACTATGTATTCTTCTGCATTATTGTTGACAATATTACAAGATATAGAAGAGCTCAAGCAAGAAAAAAATAGGCTAAAAAATAATTAAAAAAACATTTTTATAAAAATACATATAATGTATAATTAACTTACTTGTATACATTTAACGAATATCGTAAATTAGGAAAGGAGCAATACTTATGTCAAGAAGAGACCAATTAACAGGCAAAGGACCTATGGTTGGAAACATTCGTTCACACGCAATGAATGCTTCAAAAAGAAAATTTAACGTTAACTTGCAAAAAGTAACAATGAATGTTAATGGTAAAAAAGTAACTTTAAGAGTTAGTGCTAAAACAGCAAAAACCTTAAAGAACAAAGGTGTTAGTGTTGTTGCCTAATTTACCAAAAGCCTATAATAAGGCAAAATAATAAAAATTATATGTTGTTTTAGTCAACATATTTTTTTATTCCTTTTTTGCTTAATTTGTATAAAAAAAGTTGCAATAAAGCAACCATTATTTTTTACCAAATACACTAGCAATTCTTTCCTTAGTTGGTTCTGAAACAATATAAGTTCCAGCTACTGTTGCATCTACTCCAGCTGCAAAACATCTTGGTCCAGTTTCACCATTAATTCCACCATCAACTTGAATTAAATATTTGTAACCATTTTTGTCTCTTAATTGTTTAAGTTCTCTAATTTTATCAACAGCATGTTCAATAAATTTTTGTCCTCCCTTGCCAGGTTCTACAGACATAACTAAAACAAGTTGTAAATAAGGAAGTATTTTAACTATTTCTTTAACTTCGGTTGCTGGTTTAATAGCAAGTCCTATACTGTAAGCTGAATGATGATCTTTTAAGAATTGCAACAATTCTTTTTTATTAACTGCTTCATAATGAATTGTTGTAATATCAACATGGTCTCTAAATTCATCTAAGTAATCAAGAGGATTTTCAACCATTAAGTGAGCATCTTTAAAGTGTTTAGGTGCATTCTTGTTAATGTTTTTAATTTCATCTAATTCAATAGCTGTGTTAGGTACAAATTTGCCATCCATAACATCATAGTGAATTCATTTAATTCCATTATTAACTAATAGTGTTGCCATTTCACAACGTTTATCTTTTTCAACATTTAATAATGAAGGTGTTACATATTTTTCTTTTTTATTCATGTTTAATCTCCTTTAATAATTTTAGATAATTTTGATATCTAAATTCTGGTATTGTTTTGTTTTTTACATTGAGTTTTATATTGCAATATTCTTCAGGTTCATTTTCATGCAAGCATGATTTAAATTTACATAATTTTCCTAGCTCTCTAAATTGAGAATAAGAAGAAGCAAGTTCTAATTTTGAGATGTTTAAATCAAAAGAGCTAAAGCCTGGAGTGTCAATTAATTCACCATTTAATATTGGAATTATTTGAACAATTCTTGTGGTATGTTTACCCCGATTTACTGATTTTGAAATGTTTTGAGTTTCATAATTATTGTTGGTTAATTTATTAATTATTGTAGTTTTTCCAACTCCGCTTTGACCCATTAAAGAGCAAGTTTTATGATCAAATATTTTGGTAAATATTTTTACTCAATCATTTATTTTATAGTTTATTTGGTAAATTTCATAACCCATATTTTTATATTGTTCAAAGTAAGGGGTTGAACCTAAATCATCTTTAGTCAAAATGATAATTGGTTTGATATTTTTACTTTCTATAAAAGCTAAATATTTGTCAACTAAGTAACTTTGAAATTCAGGTTCTTTAATTGACATAACCACAACAATATTGTCGATGTTTGCTACTTTTGGACGAACAAAAGAATTTTTTCTAGGATATATTTCTTTGATATATTGGCCTTCTTCAAACTCAACAATATCACCCACTAAAGGTGTTATTTCTTTGTGACGAAAAACACCAGCTGCAGGTAATATGTGCATTTTATTTTGCAAATCATTTATGTAATATTTACCTGAATTAATTGAATAAATTTTGCCTTTCAAATTCTCTCCTTATTAGTGAACATAAACAACAATCAATACTAAAGCTATAATCAAAACAACTAATATTATTGAAATACCTGCAATAATAAATTTCTTGCTATTAACTTTTTCTTGAATTGTGTTTTTAGGTTTAACTTTTCTAAGATCAAGAGGTTTTTCATAAACTCTTTTAGTATCAAGACAAGTCATAAGATCACGACGCATATCTCACATAGTTGCATATCTTTTGTTTGGATCTTTAGCTGTTGCTTTGATGATAACGTTAGCTAAAGCTTGAGGTGCATTAACAAAGTTTAATAAGTTAGGAACATTTGTTGTTCTTTGCATTCTTAAAGTTTCTTGTTGGTCTTTACCTGAAATAGGATATGAGCCAGTTAGCATTTCAAATAACAAAATGCCTAAAGCATAAATGTCCGAACGGACAGATGGTTTCGAATTAGCTAAACAAATTTCAGGAGCCATATAGAAAACAGAACCGACTACTTTGTTTTCTTGTGTAAGTCTTTGACTTTCATCATCTAAAGCTAGGCCAAGGTCAATAATTTTTACATTTTTATCTTTGGTAATTAAAATGTTATTACTTTTAATATCACGGTGAATTACACCTAAGTTATGTAATTCACTAATTCCTTCAGCTATTTGAATTGCATAGTTAACAGCAACTGTTGTAGTGAATTTACCATTTCTTTTAATTATTTCTCTTAAATTTTGTCCTTCAACAAATTCCATTACAAGATATTGTTCATATTCATCGCTATAAGAGTCAAAGTATAATGCCACACGTTCAGTATTTAATTTTTTGTATATCGTGATTTCTTGTTCAAATCTTCTACGAGTTATGTCTTCATCATTTTTATTTTTATTAACACTATATTTAAGTGCAAACTTTCTTTTAATTGGGCTTTTATCATTTAAAAGTTCAACTTTATAAACTTGACTAAAATCACCATCACCAATAGTTGATAGCACACGATATTTCTTAAATATATTACTTTGTGGTTTAATTCCCATTTTAACTCCATTCTTTTAGATTGTCTAATACGACCATACCTGCTGAGCAGTTATCTGTTGATTTATTATCTAAAGCCTCTTTCACTAATTTTTTAGTCATGTCTTCAGCATTAAGATTTTCCCTTAAAATCATTTCCATTTTTGGTTTTTCGATAAAGTCGTGTACACCATCACTTGTGACTAATAAACTATAAACATGTTCGTATTGACTACTTAAGTCAAAAACTTCAATTTTAGTTTTTTTATCAGGACCTAAAGCTGAAGTTAATGAAGCATGGAAACGATTCAATTTAGCCTCATAAGGACTTTTACCTTCTTCATTAATTAACTTATTGTATAAGTTGTGATCAATTGAAATTTGCTTTAATTCACCAAAAGTTGTCATAGCATAAGTACGTGAATCGCCAATATTAAATATTAATAATAATTTTGTTTTTGTATTAAGTAAGGCAGCTGTAACTGTTGTTCCCATGTCTAGTTTTGCTTCATCATTAGCACTAATTTTAATCATTTCATCACGTGAAGCATCAACAGTTTTTTTAAATCAAGATACATAATCTTTAATTTCAGAATTTTCAGCTGGAATTGAATTGTTAAAAATTTTATTAAAAGTATTTATTGTTGTCCTTGAAGCAATCCCACCACCATAGTGACCACCCATTCCATCACATAAAATTAATAAAACATAGTTACCTTTTGTAAAGTAACTAGCTTTGTCTTGGTTTTCCAACCTTACTCTTCCCATGTCGCTTGTTATTCCATATTCCATTATTTGATACCGTATTCTTTCTCTATAATTTCTTTTATTTCTTTTGCAGCTCTTAAAGGGTCATCATTAATTATGTGATGTCTAAATATTCCTTTTTCTGCAATTTCTGCTTGCGCTTTAGCTAAACGTTTTTTAATAGCTTCTTGACTTTCTGTCTTTCGGTTAATAATTCTTCTTTCAAGTTCTTCAATTGAAGGAGGTAAGATAAAAAGTGTGATCAATTTGTAATTCTTGTTATTTGAAACCTTTTCTAATATTTGTTTTGCACCATTGGTTTCAATTTCTAAAACAGGAATTTCGCCATTTCTATGAATTCTTGCAATTTCACTATAAAGTGTTCCATAATAATTATCAAAATGAAAACTGTATTCAAAAAGATCATCATTTTGAATGTGTTTTTCAAATTCTTCTTTTGTTATAAAAAAGTAATGAATGCCATTAATTTCACCTTCACGAGGAGCTCTAGTTGTTGCTGAACATGATAAATGTAATTTTAAATTTTTAAAATCAAAAAGGTACTTTTCAATTGTTCCTTTGCCAACTCCGCTAGGTCCTGTAAATATTATTATTGGCTTACGTTTGCTAATTAAATTGTTCATAATATTCATTATATATTTTTTTACTAATATTAATAATAATTTTTAATATTAAAATTAGCAAAAATTAAAAAATGCCCTTGAGGGCACTATTTAATCTATTAAATTTCGTATAAATCTGAAAGAGTTTTTTCATTAAATACTTTGGTGTCATTAATTTCATTAAATAAGTAGAATATCATTGGCATAACATATTTTTCAACTGTTGCTTTTGAAAGTGCAATATAGTCTACCATTTTGTAGAAATTATTGATAATAAATCACATTGGTAATTTTGTTGAATCATCAATTGTTTCAGGTAATTCAACTTTGAATGGATTGTTTCAGTATTTTGCATTTGTTTGAATATTAAATTCATCTTCGCATTCTTCTTTTAAAGTAAAGATTGCTGTAAGTTTGAAGTTAAAGTAATTAACACCTGATTCTTGAGGTATAAAAGTATGAGCAAAATGAATATTATTTTTTCATAATTTAAGAGCATCGAAAAGTTTACTGTTAAGCGGGTTTTTGATTGCATGAATATTGAAGATTTTGTCTAAATCAGAAACGTTACTTTTGATTGGTTCAAGTTTAGCTTTGAAAACTTCAAATTTGTTAAATAAGTTAAATGGTAAATAAATTTTGTCAAATTGACTATTCATAAATGAGAATTCAAAAATCTTAATTTTTTTAGCTTTATTACTAATAATACTTTTGTCTGATTCAGCTGTTTCATTAGCCTTTTCGTCAATTTTTACTAGTTGAGCATCTTGTGCTAAAACATTCATTCCATATAGTGTAAAACGTTTAGCGTATTTTGAAGCTTCAACTTTGTAGCCTGCAGGAATATCAGGATATTTTAGATCGTTAGCAACAACTAATGAAGCAACATCTTCTTGATAATTTAATGTATTTTTGTTTTTTAAAACTATAGATTTTAAATCTCCAATTTTGATTTCTTCTTTTAATTTATCTTCAAAATATTTAGCCTTATCATTGTTATATCGCGTGTATTTTCTATAGTAAACTACAAAGCATATTGCTGCTGAAATAAGTGATAATATAGCTATACCAAATAGTGTATAAACTTCAGCATCTTTAACCATATAGTTAGCTGCTACATAAGCAATATAAGACACTGCAATTAAAACAGTTACTAAGAAGAAAACAATTAGTGGAATAAAGATACTTGAGATTTTTGAAATCTTACGATTAGCAATTCTGATCTTTTTTCTTTCTAATTTTGAATCAATATATTCATTCAATGAAATAGCACTTTCCATTTGTGCTTTGATTAAAAATTCGTCATTTTCATTTTTATTTTTTAATGAATTTACACGACTTTTATATTCAGGTTGAATAAAACGAAATGACTCATAAATTTCACACATATTGTGTTTGGTTATTTTGTTTTTCATACTAACTCCATATATTTTTTATCTTCATAATTATATTGAATAATTTTAAAAATATTAAAAAGCACTATAAAAAATACAATAAATTAGTAGAAAAATTAACTTAAAATTTAGAATAATAATATTTATGCTAAAAATTAACATAAATTTTGCAACTTTTCCTTTATAATCTTAATATTAATACTAATAAGAAGTTTTTAGGTTGAAAGGAGAATTATGGCAGTTATATTTGTATCAATAATTATCGCTATTTTATCGATCGCAATTATTGTGATTTCATTGCTTATGTCACCAGACAGTAATGGTTTTTCTGGTGCACTTGTTGGTAGTGGTGATTTGGAACTTTTTAAATATTCAAAAGAAAGAGGACTTAAAAAGGTTTTAAAATACTCAATGTTATTTGGTGGAATAGCATTGCTAATTTTAGCTATTATTTTAAGGATTATTGTATAACTTATGAATCCGCAAGAAATATTAAATTTTTTAAAAAAACATGATAATTCAACTTTCATTGATATTGCAAAAGGCTTAAGAATAAGTTATAAAGATAACAAAAAATTAACCGAATTACTAAATAAATTAATTGATGAAAGAAAATTAATTAAAAATGGCAAACTCTCAACTTATTCATTAATAGAAAAAATTAAAATCGTCCAAGGAAAAATTCGATTTGCTTCAGAAGGCAAGTATGCTTTTGTAGAAGAAGAAAATGATAATCCTGAAGCTGAAAAAATAAGTTATTTTGTACCTGGTATTCATCTTAATAGAGCATTAGATGGAGACTTTGTTGAAATAACAGTTTTAAAATACTTTCATTCTTTAGATGATAAAACTTTTGCAATTGTTGAAAAAGTTTTAAGCAGAAACAGTGATAAATTCATTGGTATTTTAGGTATGCATGAAGGTTTTCTTTCATTTAAACCACTTAAAAATGACTACAAAAAAATTAATTTTCATATTAAAGAATTGGTTAAAGAAGCTAGACTTCATGATGTTGTTTTAGGTCAAATGGTTTCATTTAAAAATAATATTATTGAGCTTAACATTATCAAAAAAATAGCTAACAGTAATGATCCAATGGCTTATGTTAAAGCCTTAACAGTTATTAGAAATAAACCTGAAGATTTCCCACAAGAAGTTAAAGATTACTTAGTTAAAATTCCAAATAGTATTGCAGATGAAAAACTTAATAATAGACTTGACTTGAGAAAAGAATTAATTGTTACTATTGATGGTAATGACACAAAAGACTTTGATGATGCAATTAATGTTAAAAAGAATGCTGATGGAACTTATGAATTAGGCGTTCATATTGCTGATGTTAGTTATTATGTCAAAGAAGACAGCTCTCTTGATGTTGAAGCATTAAAAAGAGGAACCTCAAGATATTTAGTTAGTGGCGTTATTCCAATGTTGCCTCATAAATTATCAAATGGTATTTGCTCACTTAATCCTAATGAAGATCGCTTTACTCTTAGTGCAATAATGACAATTGATAAAAATGGTGATACTATTGATGCTAAATTTTATCAGTCAATAATTCAATCAAAATATCGTCTCACATATGATAGGGTTAATGAACTTTATGATAAAAATCAAAAATTTGATGACCCGAAATTAAATCGAATGCTCTATGAAGCAGCTGAATTGGCAAAAATAATTAGAAATTATAAAGTCAAAGAAGGCTACATTGATTTTGAAATTGAAGAACCCGAAATTGTCTTAGATGATAAAGGTAAAACAATTGATGTTGTAGTTAAGCCAACTGGTTTTAGTGAAAATCTTATTGAAGATTTCATGGTTAGAACTAATGAAGAAGTAGCTAAATTTTTAGCTAAAAGAAAAATACCTTTTATTTATCGTGTTCATGAAAGCCCTGATAGTGACAAAATTGATTCATTTTTGAGTGTACTTAATGTTTTAGGTATTAAAGTAAACATTGATCGAAATGATATTAATCCAAAAAGTTTTCAAAAAATAATCAACAAAATTAAAGAAACAAGAAATGATGACTTTTTAAAAACAGTTTTCTTAAGAACAATGCAAAAAGCTAAATATTCTCCAAATAATATTGGCCACTTTGGTTTAGCAAGTGAACACTATTGTCACTTTACTAGTCCAATTCGACGTTATCCAGATTTAGTTGTTCATAGGATTTTAAGAGAAATTGTTTTTAACAATGATAAAAGTAAAATTACTCACTACAAAAACATTTTAAGTAATGTTGCAGAATTAAATTCAAATAGTGAACAAGAAGCATTACAACTTGAACGTGATACAAATGATTTAAAATATGCTGAGTTTTTCAAAAACAAAATTGGTCAAACAATGAAAGCTCAAATTATTAGTATTTTACGCTTTGGTATGTTTGTCCAATTTGACTGCAAAATAGAGGCTTTAGTTCATATTTCAACATTATGTGATGGTGATTATGAAGTTAATGAAAACTTTACTGAATTAAGAAGTAAACAACACACTTTTAAACTTGGAGACCAAGTAGAAGTGGTTATTGCTGGAGCTGATGAAACTAACGGTAAAATTGATGCAGTGCTTGCAAAATGCTATGGTTCATACTTAGAAGAAATCAAGAAAAAACTTAATTCAAACTCAAATCGCAAACATAAAAAATCAGGTAAATAATTATGAATAAAAAACAAAAATTAGTTAAAAATTCTTTAGGTTTTGATTCTAATTTATATGTTGTGCAAGACAAAGATATGTTCAACTATTCAGTTGACACTATTATGCTTGGCAACTTTGTTTTTTTAAATAATAAAATTAAAAACCTATTAGAAATTGGAGCAAACAATGGAGCTCTCAGTATTTTTATAGCTGAAAGAAATCCAAATTTAAAAATAGATGCAGTTGAAATCCAAACTAAAGCTTGTAAGTTAGCTCAAGACAATATTGAACTTAACAACAAGCAAAATCAAATCAAAATAATTAATCAAGATTTCAATGAATTTTATTTAGAAAAAATTAAAAATGCAGCTAGTAAATATGACTCAATAGTTTGCAATCCTCCTTTTTATACAATGCAAACTAATAAAGTTAGCAAAAATATTAGTGAAGAATTACTAATTGCAACACATGAGTATAAAATTAATTTAGAACAAATTATTTTAGGTTCTTCAAAATTAATTGAGCAAAAAGGCTACTTAACTTTAGTGATTCCAGTTGAACGCTTAGTTGATTGCTTTTGCTTAATGAGAAAATATAATTTTGAGCCTAAAAGAGTTCAATTCATTATTCCAAGAATGCAAGATAAACCTAAATTAGTTTTAGTTGAAGGCAGATATCAAGCTGGTTGAGGTGTTCATTTCTTGCCTAACTTGTACTTGCATGATCCTAATGACAAACAGCAACATGAATATTTAGAAAATATTAAAAAATTGTACAAACCAATTAAAGTTAAAGAAGGTAAATAAACATGAAAAAACAAAAGACTTTTTATATAACTACTCCTATTTATTATGCTTCAGGTCCGCTTCATATCGGACATCTTTATTGTACAATTATGGCTTGAGTTATTAGAAACTATAAAAGAGTTTTAGGTTATGATGCTAAGCTTTTAACCGGAAGTGATGAACATGGTCAAAAAATCCAAGAAAAAGCCTTAGTTGCTAAAAAAGAACCAAAACAATATGTTGATGATTTAGTTAAAAGTTACAAAGAAATGTGAGCTAAATGAGAAATCGATTATGACTATTTCAGTAGAACAACAGAATCATTTCATGAACAAGCTGTTCAAAAAATATTTTCTTATTTTTTACAAAAAGGTTTAATTTATAAAGGTTCATATCAAGGACTTTATTCAGTTCAAGATGAAGAATTTTTGACAAAAAATCAAGCTGTTTATAAAGATGGTAAGTATTATCATCCTTCATCAAAACATGAATTAATCAATATGGAAGAAGAAAGTTATTTCTTCAAAATGAATGAAGTTCAAAAATGATGAAGCAAATATAACAAAAAACATCCAGACTTCTTATTGCCTAAAAAAGTAGTTAATGAAATGACAAACAATTTTGTTAAAGAAGGCTTAGAAGATTTATCAGTTACAAGAACAAATGTTAAATGAGCCATTCCAACAATTGATGATCCTAAACATACAATTTATGTTTGATTTGATGCTTTATTTAACTACATAACAAAACTTGGTTTCGACTTTAATAAACCAAAAGATGACTTTGTTAAATACTGAAAAAATGGTGACGAAATTGTGCATATCTTAGGCAAAGAAATAGCAAGATTTCACTTTATTTACTGACCAATTGTTTTAAATTGTTTAGGTATTAAATTACCAAATCATATTGTTAGTCATGGACTTTTAAGAGATAAAGATGGACTAAAAATGTCCAAATCTTTAAATAATGTTATAGCCCCAGATTATTTATTTGACAAGTATCATCATGAGATGATTAAATACTATTTTGCAACTCAAATAATATTTGGAGAAGATGGTAATTTTAGTGAAGAAAAATTAAAAGAAGTTGTTAATGCAGATTTAATAAATAATTATGGTAATTTAGTTTCAAGAACACTAAAAATGATTCACAATTCTTTCCCAAAAGGTCTGCTTTATAGTGTTAATAGTAAAAAAATACATAAAGACATTGAAAAAGAAATTAAGAATTTCAGTGCTCAATTTCAAAAACATATGGATGCTTTTGAAATTGATAAAGGATTTAAAAAAGCAATTGAATTATCAGCTAAATTAAATAAATATATTGATGAAACAACACCATGAAAATTAACTGATAAATTAGAAGAATTGGAACCAATTTTAGTAAGATTATTAAATGGAATTTATGCTGTTAGTTGTGCATTACAAATAGTATTGCCTACAAAAATAAAAGAAGTTGCAAGTGTTTTAAAAGTTAAAAAATTTGACTTTGAAAAAATTAATGATTTCAATAAATTTGATAAAGTTATTTGTGATGAGAAATATTTGTTATTTAATAGATTAAAATAAAGGAGGAAGAATGATTTATGCAATTTTTAGAAAAGTAAAAATCAAGTTAGAAAGCAAATCGATTTTTGAAAAAACTGTTAGTTTATGAATTGATGAAGTTAAAAAAGAAGAATACAACTTATCTTTTGATGGCTACTGAGTAAATGATAATACATTCATTTTTATGGAAAGATGAAGTACATTAGAAAAATATAAGAAGTTTGCTAAAAAAGAAGAAGTAATTTTACAATGAAAATCTTTAGCTAAATATTTTAGTGAAGAAATTAAGACCTATGAAGGCAATACTATTTGTTAATAATTTAAGGCTCAAAGCCTTTTATTTTTTATTTGTGAGAGCATTTGCACAAGATTCATTTTTTTTGCTTACTTAAATGATTTTTTTATTTAAAAAAATATATAATTAAAATGTATTTATTCATATGAATACAACGCTATTGCGTATAAAAAGCTTTTTATAAATTTACATTTTTTTAATTAAGGAGAAAGATGAAAAAAGTTGCAATCAACGGTTTCGGTCGTATTGGACGTTTAACACTTCGTCACTTACTTGAAACCCAAAATAAAGAAGTTCAAGTTGTTGCAGTTAACGACTTAACAGATCCAAAAATGTTAGCACATTTACTTAAATACGATACAGCTTTTGGCCCATTGGAAGCTAAGGTTGAAGTTAAAGAAAATGCTATTAAAGTTAATGGAAAAGAAATTAAAGTATTCTGCGAAAAAGATCCAGCTAACTTACCATGAGGTGAATTAGGAATTGACATTGTTTTAGAATGTACAGGTTTCTTTGTTAAAAAAGAATTAGCACACAAACACATCGAAGCAGGTGCTAAGAAAGTTGTTATTTCAGCTCCAGCTGGTAAAGATGTTAAAACAATCGTTTACAACGTAAACCACGAAACCTTAACAAAACAAGATGAAATTATTTCGGGTGCTTCATGTACAACAAACTGTTTAGCACCAGTTGTTAAAGTTTTAGTAGACAAATTTGGTTTATTAAACGGTTACATGACAACAGTTCACGCTTACACAGGTGACCAAATGTTACAAGATGGTCCACACCGTAAAGGTGACTTAAGACGTGCTCGTGCTGCTGCTCAAAACATTGTTCCTTCAACAACAGGTGCTGCAAAAGCTATTGGTTTAGTTGTTCCCGAAGCTGATGGATTATTAGATGGTTCAGCATTGCGTGTTCCTACAATTACAGGTTCATTCGTTGACTTAACAGTTCAATTGAAAAAACAACCTACAGTTGAAGAAATTAATGCTGCATTCAAAAAAGCAGAAAACGAAACAATGAAATATGTAGTTGACCCTATTGTTTCTTCAGACGTTATTAACTCACACTATGGTTCATTATTCGATTCAACTTTAACAAAAGTTAAAGAAGGAAATGGTCAAAGACTTTACAAAATCTTTGCATGATACGATAATGAAATGTCATACGTTTCACAATTAGTAAGAACATTGTCATACTTTGCAAAACTAAAATAATCATTTTTTTCAAGGCACTCAATATGAGTGCTTTTTTATTTGCCATTTTAACTTTTTAGCATTTTGATATAATAGATATATAGAATTAAAGGAGAATTTATGAAGAAAAACTTATTAAAGTTAACAGGAATTATTGCTCCAGTTATTACAGCTATACCATTTGTTGCTTCATCATGTGGTAGTAAAGATGAAGCAACTTTAGCTGATCTTTTAAAGGAAAAATTGACAAAGGATGCAGATGCTTCTAAATATTCTGCACTAATTACTGACAAGGAAAAATTAAAACAATTCAAGGAATTAGTAGAAGAATTTAAAAAAGAAGGAGTAGATTTAACTGCTAAAGGTAATCCTTCTGATGTAGATAATGCAATTAAAACTATTAAAGAAAAAGCGCCATTAGTTTTAGCTAGAACAATTAAAGCAATGGTTGAAAACGGAAGTGCTTTTATAATTAAAGAAGATGGGATTGCTAGTGCTAAACAAAAACTAGAAAATGGTGATATATTTGCTCAAGTTTATCCTGCACAACATAAAATCGGAAGATCTGCTTATACAATTATTGAATTTACTGAAAAATTTAAGGCATTTTATTTAACTTCAAAAGTATTCGAAGTTGCTAGAATTTCAACATTTTATGAAAATAAATATGGTGGTAAAACTCAACCTCATGCAAACGGAGTAAACTTAGAATTTGTTAAAGGCAAGTTAAATATTAAATTTGTGATTTGTGATTTGCTAAAAGGCGACCAAACTGGTTCAGTTGTTTTAGTAAATAACTTATTATTTGCAGTTGAAAAATAATCAAAAATAATATTTTGCGAATCTTGGAAAGTAGTCCAAAATTCGCATTTTTTTTGGTTTTTTCAGCACTTAATGATGTGAAAAATTCCACAAATTTTGAGTCAAAATAATAAAAAATGCCTATTTTTAAGGCTTTTTAATCAAATTTATGTGTCGTTATAAATTGTTCTAATTAATATAAATATTAAAATATATATGGAACTATGTATTAAGGTTCCACTAACGTATATACGTGAAAGGAAAAATTATGTTCAAAAACATGACTCGAAACAAGTGGATTATTTTTTCACTTTGAATGGTGTTTCTTGTTTTTATGGTTTTAATTATTATCTTAGCTTCTATTAAGAACTTCGATGCTGCAGAATCTTTAAAAACAACAAATGCTAAATACGTAGCCATTGTTGCCTTATTTTTCTTTATATCTTTATGTGCTGCAATTTTTGGAACAGTTATTTTATCAATCTTAGATAAAAAAGCTGTTACAAAAAAAGCACAACAAAATCAAGGAGGTAAATAATGACTGGTACAAAAACACAATCATCAAGACCTTCATTTGGAAAAAGACTATTAGAAAAACTTGGACGCATTGGTCGGGTATTGATGTTCCCTATTGCAGTTCTTCCTATTGCTGCTATTCTTCTTAGAGTTGGAGCTGATTTACCAACAGTTAAAGCTGGACCTGATATTTCACAAGTCGAAGCTAACTTTGTTAACTTTGTTCAACAAATGATAACAGCAGGCGGATCAGTTGTATTTGACAACTTGTACATTTTATTTGCCGTCGGAATAGGATTTGGTTTTACTAAAGATAACCGTGGTGAAGCTGCTTTTGCTGCTTTAATTAGTATTCTATTACTTTCACTTTTATTAAAAAGTGGTGGATATGTAGATAAAATCTACAGCACATTTAAACTCCCTGGAGCCGAATTTAATGAAGATGGTAAAAAACTTGAAGCTTTTATAGCTGAAGGTAAACCTGCTTCAGAATTTGTTATAGCTAATGGTTTTGCAGGTGTCTTTGGTGGCAAATACAATGCAATAATGTCTGGCAATGTACTTAATGGTATTATTGTTGGATTTATTGTTGCAGCTATTTATAACCATACAAATGATGTTCAATTACCTAAAGTTCTTGGATTCTTTTCGGGTAGAAGATTAGTTCCAGCTCTTGCAATTCTTTCAACTCTTATTTGAGGCATTGCATATGCTGCTATTTTTCCATGAATTGGATATGGTATTTATGCATTTTCAAAAGTGTTATCAGGTGCAGCTAAAGGTCGTTGAGCTTCAGCTGCTATTATGGGTGTATATGGCGTTATTAATCGTCTATTAATTCCATTTGGATTACACCACATTCCAAACAACTTATTCTGATTCCAATTAGGAGAACATGTAACTCCTGATGGTACACCTGTATTTGGTGATATCTTTATTTTCTTAAGTGGTAAAGCAAAAGGAAACACAGCTGGTATCTTCCAATCAGGATTCTTTCCAGTTATGATGTTTGGTCTTCCTGCTCTAGCATTTGCTTTCTACATGACTGCAGATGATAAAGTGCAAAAGAGAAGAGTAATGGCTATCTTTGGAGCAGCCGCAGTAGTATCAATTCTTTCAGGTATTACTGAACCTATCGAATTTGCATTTATGTTTGTTAGTCCATTATTATATGGTTTCCACTGCATTCTAACTGGTATATTTGCATTTATAACTGGTGCATTTGGAATTCAAATTGGATTTGGGTTCTCAGCTGGTTTAATGGATTACTTATTATCAATTCCTAAATCATTAGCAATTATTAAAGCTAACAAAACAGGTATTGATGCTGTATTAGCTAACCCTGGTTGAATCTTAGTAATTGGTGTTGCATGTGCAGCTACATATTACTTTGTTGCTTACTATACAATTAAAGGTCTTAAACTTGAAACTCCAGGTCGTGGTGCTAACCAATTGCCTGATCCAAATGCTTTAACAGAAGTTAAATCAACTTCAAGTGCTTCTGGTGAAGAAACTCCAACAGATTCGAAATTAAATAACCTAAGTCTAAAAGCAAAAGCTATTGTTGAAGCTGTTGGTGGTTACGAAAACATTACAACTTTTGCAAACTGTGCAACAAGACTTCGTTATGAAGTTAAAGACTCAGATATTGTTGATGAAGCAAAATTAAAACTTGCGGGATGCAGTGGTTTGATTAGATTAGGAAAGAATTCAATTCAAATTATTGTAGGTGTTGAAGCTGAAAACATTAACAATGAAATTGAATCAAATAAATAATATAAATAAAAGATTACTTGCTTATTAGCAAGTTTTCTTATACTCTTATTTCTTTTTCTTGTATTCTTCTTTTGTTTAAAAATGCAATATCAATTTTTTGTATATTTTGAGAATTTAAAAGATTTTCATTGTTTTTGATTTTTATTGATATAGGTATAAAAACTGTGTTCATAAGATTTTTATTTTCGTTTAAAAAATGATTATTATCTTTTGAATTGGTGGTATATAAATCTCCCAAAATTCCATAAATAATACATCTTTGATTTTCTTTATTGCTTTTTGTAACTAAAATCAATTGTGAATGGTTATAAGCTCCATCAATATCAAATTTTGGTTTTAATTTAAAAAATTCAAATGCAAAAGTAAATAGTTTTTCTTGATTAATATTTTTTATAATATTGACAAAAATCTCAAGAGCGTTTAATTTTTTGTCAATTAAATCTTTTTTGTTTTTGCTTATGTCTTTAATTAACTCATCATAATTTAGTTGATCATTTTTTATATCTCTATAAAGTTCAAAATTATTGTTCTTATATTTTCTAAATAACTTTTTGTTGTATTGCAAACCTAGTGAATGCAGTAAAAATTTTTGTGTTAAAACTAATCTTAATTTAACATCAAATTGTGATTCATTATTTGCCTTTTTTAATAATGAAATGCTTTTATACCTATAAAAGAATTCAATATATTTAGTTAATAAATTCTTAAAATTTTGATTATTCATTTTTTTCTCCTTTATTTGTCAATTTATTGATACAAACAAAAATCTAAATTTAATGTAAAATATTTACATTATGATAAATGACACAATTGCAGCAATATCTTCAGGTGGTAAGATTAATCAAGCCATATCAATAATCAGAGTTAGTGGTCAAGATAGTATTGAAATAGTTTCTAAAATTTTTAAAGGAAAAATAGGAACAAATCAAACAATAACTTATGGCCATATTTTTGATAATAAAACAAAAGAAATTATTGATGAAGTTTTAATTGCTTGATTTATAGGAAATAAAAATTTTACAGGCGAAAATACTGTTGAAATTAATTGCCATGGTGGTGTATTAGTAACCAATAGAATTCTTGAGTTATTATTAGCTAATGGTGCTCGAATGGCACTGCCAGGTGAATTTAGTCGACGTTCATTTTTGAATGGTAAAATGGACTTAATTAAAGCTGAAGCAATTAATGATTTAATACATGCCAAAACATTAAAACAAAGTGAATTAGCCATTAAGAAATTTGATGGCAAAACTTCAAACATGATTAAATCATTTATTGACGAATTAGCCTATTTAATAGGTCAAATGGAAATAAATATAGATTATCCAGAGTATGAAGATTTTGAAAATGTTTTGACAAAAGATTTAATTTCTAGATTGCAAAAATTGCAAGACAAATTGAGCTTAATTATCAAAGAAAGTGAAAACTCAAGATTAATTTTTGATGGCATTAAAGTTGCTATTCTTGGTAAGCCAAATGTTGGAAAAAGTTCAATATTAAATTGCTTAATAAATGAAGATAAAGCAATAGTTACAGATCAGGCTGGAACAACTCGTGATCTTGTTGAAGCTTCATATCAAATTGATGGTTTATTATTCAAATTAGTTGATACCGCTGGAATTAGAAAAACTAATCGAAAAATTGAAAAAATAGGTATTGAAAAATCTTTTGAACAAATTGAAAAATGTGATGTTGTAATTCATGTTAATGAAGCTAATACTTTAGAAAATGATTACGATAAAAAAGTTGAAAAATTAGCTAAACAATTTAACAAACCATTAATTAAAGTAATTAATAAAGTTGATTTATTAAAAAATAATAAAAAGAAAAAGAATGTTATTTATGTCAGTGCTAAAAATAAAAACATTGATGATTTAAAGGAAGCATTAGTTAATATTTTTTCAAAGAATGAAATTAACAATGAAGAATACGTTGTTAATGCAAGACAACTAGCATTGGTTAAAAAGGCTCATTCAAGTATTTCTGATGCAATTGAATCAATAAATAATGGCATTGATGTTGATGTAGTAATTATTGATGTAAGACAAGCTTGAGCTGATTTAGTTGATATTTCAGGTAGAGCTGACAATGAATTATTGCTTGACGAAATGTTTAAAAACTTTTGTTTAGGAAAATAGAAATAAGAAAGGAACAACTATGTCAATAAAATATATTGATGCTCATACTCATCCATTAAAAGAATACTATGATGACAATTATCATGTAATTGAAAGAGCTCATGCTAAAGGTTTAGTAGCTTTATTAATTACCGGATGTAATGAACAAGAAAATGAAGAAGTTATTAAAATTGCCAAAAACTTTTCATATACTTTTCCTGTTATTGGAATTCATCCAAATGAATGTCATGGAAAGGTTGACGGCGAAATTATTGAAAAGCAATTAGATGATTCTGTTGTTGCAATTGGCGAAATTGGTTTAGATTACTTTTATACACCAGAAAAGAAAGATATTCAAATTGAATCATTGCATGCTCAAATTCAAGTAGCTAAAAAACACAATTTACCAGTAGTAATTCACATGCGTGATGCATATGAAGATTTGTACGAAATTATTAAACAATATTATAAAGATGTTGTTTTTATGATTCATACATATAGTGGTAACTTATATTGAGCCAAAAAGTTTTACGAATTAGGTTGTTACTTTTCTTTTAGCGGAGTGGCAACTTATAAAAATGGAACTGAAACTATTGAAGTTTTAGATTGATTGCCAATTGATAGAATATTAACTGAAACTGATGCTCCTTTTCTTTCTCCAGCTCACAAAAGAGGTGAATTAAATTATCCAAATTATGTAATTCAAACAACTCACTTTATTGCAGGTATTAAAAAAATACCAATTGAAAAATTTACTGACCAGATTTTAAAAAATGCAAAGGATTTATTTAAGATAAATGTTTCAAGAAAATAAATTAAAAGCCAAAAAAAGATATGGTCAAAATTTTTTAAAAGATCAAAATGTTATTAACAAAATAATTGAATTGATTAAACCACAAGGTGAAAAGATTTTAGAAATAGGCCCCGGAAGAGGTGCTTTAACTAAAATTTTAAATCAACAAGCAAAAGAATTTACTGCTTTTGAAATAGATCAAGATATGGTTGATTATTTACAAAAAAATAATATCTTAAATCAAAATCAAATTGTCCAAGGAGATTTTTTAATAGCTGATTTGAAAAAATATAAAAGCTATGTTGTTGTTGGGAATATTCCTTATTACATAACTAGTGATATTATTTTTAAACTATTAGATTATCGACACAATTTTAAAAAAGCTATTCTTATGGTTCAAAATGAAGTGGCTCAAAGATTAGTGGCTAAACCTAATCAAAATGATTACAGCAAACTTTCAATAACAGTTCAATATTGTGCTGATGTTAAAAAAGAATTATTTGTTAAGAAGACTTATTTTGATCCAGTTCCTAAAGTTGATTCTGCTATTGTTTCTATTACTTTTAAAAATGAAGCAAATGATAATTATGAAAATTTAAAAGACTTTTTCAAATTATGTTTTTTAGCTAGAAGAAAAAAATTATCATTTGCTTTAGCTACAAAATATAGTAATGATAAAATTAAAATTGCATATAATAAATTAAATTTAAAAGAATTAACTAGAATTCAAGAATTAGATTTAAAAACTATATTAGCTTTATACGATTGCTTAGAAAATTAGGAGATTTATGGACTTAGATTTAGAATATGAAAAAGAATTAATTGTTGTTTGTAAAAACTATCATGAAGAAGCACAAGCTTATAAAAATGCCAAAAAGTTCATAATTATTTTAGTAACGCTTTGCTATCTTATTGCTTTTGGACTTTTTGCGGTTTGATTTACACTTTCAATGGTTTTTGGCTTTGGTTATTTTAATGTTGGCGCACAAGTTGCAATTTTCTTATGTTTCACAGTGTTCACATTATTTGGAACTTTGCAATTATCTGGTTGAATTTTAATAATGAAAGCCATCAAGTATTATGAAAGTGATCAAAAAGAAAAAGCTATTAAATACTATAAAGCATATTGTACAATTGCTTTTAAACGCAAAAAAGATTACAGTGATAAAAATTCTAATAACAAAAATGATGATGACACAATAATAGTTAAATAATTTAGGAGCATTATGAAAAAAGTAACAATTATCGGAACAGGTGCCTGAGCTTCAGCACTCGCAACTGTTTTAAGTAAAAATAATAACAAGGTTTTAATGTGAGGAATTGATTCAAGTGAAGTAGATTCAATTAACAAAGGAAATAATAGTAAATATTTTAAAGATGCTTTATTTAATAATCCTTTTAATGTAAAAGCTACTTTGGACTTAAATGAAGCTTTAAAAGAATTTGATTATTTAATTTTAGCTGTACCTTCAAGTGCAATTACTTCTGTGTTAGAACAAATAAAGGAAACAATCAAAAATAAAAAAATAAATGTTATTAATGTTGCAAAAGGTATTGATGAAAAAACAAATGAATTTTTTTCAGAAGTTTTAAACAAACAATTTGCGAATAATTTAAAAAATTATTGTACTTTAATTGGTCCTTCTTTTGCTGTTGAAGTTTTTGAAAATGTTTTAACCATGATTAATGTTGTTGGTCCAAAAACAAGATTTTTAAAAAAAGTTTCTGAATTATTTAATAATGACACTTTTAGACTTGTTGTTAATGAAAATGAACACGGTTCAGAATTGTTTGCTGCTCTAAAAAATGTTCTAGCTATTGGAATTGGAATGATTGATTATTTAAGACCATATCGAAATACAATGGCGGCCTTGCTTTCAATTGGTGTTAAAGAAATTCACATGGTTTACAAGAGAATATTTCCTGAATCAAATGACAATATGGGATTTGAATTAGCAGGAATTGGCGATATCTTTTTAACTTGTTCAAGTACAAAAAGCCGTAACTTTTCTTTTGGTAGACAAGTTGCAGAATTTGGCTTGGAAGCAACTTTAAAAAATAATAAGAAAACTATTGAAGGATATCATGCTGCTAAAATATTAGCTAATATTCTAAAGAAAAATAAAGATCTTAAAATTGTATTTTTAAAAAGCATAATTGATATTTTATATAAAAACAAGAAACCTGAATTACTTTTAGATTTTGTTAAAAAATATAACTAAAAGTTTGTTTTATAGTTGTTTTATCAAAATTGAAAAACAATTTTAAGGCTTAAAATACCAAGTCTATAAATAAAATACAAAATAAATATTTTTTGTGGTATTATTAAAAAGCAAAAAATATTAAATTTATTAATGGAGAATTTGCAGTTTTAATTGACAAAAATAGTAATTATTTTTTTCTTTAAATAAAAAAATATAATTGTATAATTAGTTTTAGTCGTATATTATATAATTATTTGCAGATGACACCATAGCCAAATGGCCAAGGCATGAGTCTGCAAAACTCTGATTACGGGTTCGAATCCCGTTGGTGTCTCCATTTTGTACCCATAGCTCAACTGGACAGAGTGTCAGGTTACGGCCCTGAAGGTTGCGGGTTCGACTCCTGCTGGGTACGCCATTTCATCAGTGATGACCCTTAACAGCTTAGGCTGTTTTTATTTTTCAAATTTTTATATTAGATTTTAGCTTCATAAAAAAGATTGTTAAATATCAAAAGCTACTTCAAAAGATTAATATTCATCAATTGATACTTATATTTATTTTTAAAGCAAAAGTGTATTCAACTAAGTAATATAACAAATTATCTAACAAGTAATACATTCAACTCAGCATTGTTGGACTTCATCAAAATAATAAAGAACACAAGTGAAAAAATTGAAATAATGGAGCCATAATTATTCCTAGTAATAATCCGAAGTAATTTCAATATTCATTAATATATAAAAGCATTGGAATATTAAAAAGATATATTAAAGTCATCAAAATTAATGTTTTTAAAAAGTTGTTTAATTTTTTACTTTTGACCATTGGCACTAATAACATAATTGTAAAAGTTGATGAAAAACTAAGAATAAATGATAAAGAAAACAAGTAATTATTTTTAAGTAAAAATAAAACTAAAATTGAAATTACTAGACCATCATATTTTTTAAATTTGTTGTTATAACTAAAAGGATTATTTGAATTTTTAACAACTTGCATAATATAAGCTCTTAAAGCAGAAACAGGATTAGCAAGTAATGTTAAATAATAGAATATGAGGGCAAAAGCCACATATTTACTTTTGATTTTAATTTTATCCAAAGGTTTTAGTATCAACAAAATTAAATAGAATAATAAGTCAAAATGCAAACCTGAAATAACAATTAAATGAACAATATTAAGTCTATTAACTTTATTCAATACTAATTCTTTTGAATCATAGGATCCAAAAACTAACAACCTTCAATATGAATCAAATAATTCACCATGATTTTTTGAATAATTCATTATTTTTTCTTCAACAGAAAATTCTGGTTTAATAACAGTTAAAATGACTGTTTTTTTTAGCAAATAATTTATATTATTTTGCAAATAAAATGTTGCTATTTTACTATTGTCTATTTCAATAGGATTTAATTCACCTTGAATTTCTACTTGGTCCCCTAGACTTAAAGGAGTGCTATTTTTTAAATCACTTGTGTAAATTAAAATATTATTATTTTCATTTGATATAACTAAATATTTTTTGCTTAGGACGCTTATCGATCCTTCAATTTTATAAATACCTTTTTCATAATAAATTTTTGTTTTAGATAAAAAATAACTTAAATATAAAGTCAAAAGCATGGCTACTAAAATTAAATATTTAGGCTTAATTAAAGCTAAGAAAGTAACATAAATTAAAAATAATAGTATTCAAAATATTTTGGTTTCTTGAGCATTTATTACTAAGTGTAATAAAACAGGTGAGAGTAAACTAAAAAGAAAAAGACTTACAGAATTAAAATTCCTTGTAATTTCTTTAAGGTTACCTTGCCAACTCCCGAAATATTTTCTAAGTCGTTCCATGTTATTTGATTTTTCTTTTCTCTTAAATCTAAAATCTTGTTAACTATTGATTTTTTAATGCCAAAATCCACTAACAATTTAAAATGTTCTTTACTTAAGTTATTTCAATACAAAAAAGGGTTATTAGTTTTTTGATATGGAATGTAAATATTAAGATTTTCTTTTAATTCTTTTTTTAAGTCAATTTTGCTTAAGTCTGCATTTTCATAAGGTTGAGCCATATCTAATAATTCTTTTAAATAAATAGGCTTTTCGAAATAATAATTTTTAGCAAAGGCAACTGCACCGGATACTTTGATACAAAATTTTGATCCTATTGATGCAGGATCATTGCTTTTAACCTTTGCAACCTTGGTTCTTTGAATATTAAAAGCAATTGTACTTGTTGAAATTACAATTGTGCCTGCTAACAAAGTTCCTAATATTATCTTTCTATATTTCATTCCATTTTATACTTTAGCTTTTTTTAATTTTTGGTTGATTCTAGGAAAAATTTGGTATTTTAAGTCTATTTTTCCTAAATTTTTCCATTTTTGATATGTTTTTCATAAAAAAGTTAATTTTAATTTGAGGTGTTTCATGCAAAAAAATAGAGGAATGTTATTAGAAAAAATAATTAATAAAACAATTGATTACTATGCCAAAAATAAATTAGCTTATATTGAAAAGAAGTCTTTACCTGTTAAGTTTAAGAAGGTTAAAAATGATAATTTGAAATTGGATGAGGCTTATATATTAAAGAAGAGCACAGTTGATTACATCGGTTGCTATAAAGGATTTTTTGTTGCTTTTGAAGCAAAAAGCACAAATGAAAAAAGATTGCCAGCAAATAATATTGCTCGGCACCAAATTAAGTATTTATTGGATATTGAAAGTAATAGTGGATTAGCTTTCTTTATTATATTTTTTAGTTTTTGTGATGAGTTTTATCTTGTTAAAGCAAGTGAGTTAATTCAGCATATGAATAAATCTATTTCTTATGAAGAAATAAAAAACATAGGTTTTAGAATCGAATTAACCTTTCCAGGTATAATCGACTTTTTGCCCTATGTTCAAAATTTTTATTAGTGATTGTTGATATTAGAAGTTTACAATATCTCTTAAGTATTTTGAAGGTGTGAATTTTACAACACGTTTTTCAGGCACTTGAATTGGTTCATTTGTAAATGGATTAATTGTTTCACGTCCTCTTCTAATTTTTGTTTCGAAGGTTCCTAAATCTGAAAGTTGAACTTTATCTTCAGCTATAAGTTGTTCTTTTAAAACGAATAGAAATGCATCAAAGTATTGATCTGCTACTCTAACTGGAACTTCTAACATTTCAGCAATTTTTTGTACAAATTCTTTTTTGGTCATAATCTTCCTTTCTTGATTTATAGCATAATAATCATACTATATTTTTTATTATTTTATATTTTTTTACTTATTTATAGTACAAAAAGTCAAAAATACACTTTTGCATATAGCTTTTATAAAATTAAATAATTTAGCATACTTTGTTTAGTTAAAAACTAAAAAATAAAAAATGACTTTTTTATTAAGCCATCTTTTTAGTTATCTTGTGCTTTTTCACGATATGGAATTACGTGTACGTGTGTATGGAAAATTGTTTGATCAGCAGCTTCGCCTGTATTAATTATTATTTTGAAGCCTGCAATTTTCTTATCTAATACATGTTTTTTTCCAAGTTTACGAGCAACATTTATTAAATGTGCAGCTGTTTCATCATCTGCTTCAGTAATGTTTTTACTGTGTTTTTTTGGAACCACTAAGAAATGTCCGGGTTGAATTGGGAATTTGTCATAAAAAGCTATACATTTATCATCTTCAAAGATAATATTTGCTTCTGCTTTTTTGTTAATTATTAATTGAAATATGTTTTGCATAATTGACTCCTTATTTTTTGTTAGAGTCTTTGTCTTTTTCGCCTTCAACAAATTTTTCACCCTTAAGTACTTCTTTTAATGCATCATTTTCTACTTTAAGTTTGTAACCTAATTTAGTGAATGCAGTTTCTGCTGTATCAACTAATGAAGAATCTTTTAAAGCAAATAAGATCGCTAATTTTTTAAGATGATTTTGGAAGATTGTGCCTTTGAATGAAAGAAGTTTAACTTTTTCAGTTTCTTTTTCATTTGATTCTTTTGGTAATTCAACTTCAGGTTGTCCAATAGGGGCAATTTGGTTTAAGTAAGCAGCAACAATTTCTTTGTCTGATTTTGAGCCTTTATATGGACTGTAACCTTTTTTAAATAATTCATCATCTTGTAAAATTAGTCTATCTTCAATAGGTGTATAGAAACCATAAAGTTTGTTAGCTTCAGCATTTTGTAAATTCTTTTCATCTCATTTCAAGTTGTAATTACTGAAGTTTAAGTTAATACCTTGATAGCCTAATAATTGACTTTCGTATGCATTATCAGTAATTTTTTCAATGTCACTTATTTTTTTACTTTCATCTTCCGAACCTTTCATGAAGGTATTAAATGTGTCAATACTTTTAATTGTTTTTAATTGAGTAACAAAGAAATCTTCTTCAGATGTAATTTTAGTTTTTGCAGTTTTCTTTCAAAGTTGAACAGGTTTCTTTTGAACTGCATATCTAGTTAAATTATAGTTTTCTAATTCGTATTTTAAGAATGATGAATTGTATTTAAATTTGTCATCAAGTTTGGCTAATTGTTCTTTATTACTTATTTCAAAGTACTTTAGGACTAAAAGTATTTTTTGAATTTCGGCTCTAATTCCTGTTTTTTCAGCATTTCAAAAAATTCTAAAAATTTCTTCATTAGGAATTTCGCCAACTTTTAATAAATTTGATATTTTTGATTCTTGATCTTTAGTAAAGTAATTTTTTTTCTTTCAATCTAATGATTTTTCATAAAAGTAATAACCATCAGCTCCATTTTTGAAAACTTTATCTTCAGCATAAACTTTAAAAATTTCTAAAGAATCTTTATAAAGTTGTGAATTAGTATCATTAAATGTTTTTATGTATTCAGAGTTATCAGTGTTATATGAATTAGTTACGTTTATCTTATATAAAGAAGCAATTGTTATTTGTCTTCAAATGTTTTTGATTTCTTCTTTTCTGCCGCCAGCTTTTAAGAAATCATCTTGAATTTTTTTAGCTGGAGTTTCAACAACACGACCACAACTAACAGCGGCTATAGGGAAGGCTGCTAAAGTGCTAATGCTTCCTAAAGAAAATAATAATTTTTTATTTTTCATTATGCACTCTCCTTCCTAGTTAAAAACTTTCAAAATTTGTTTAATTTTTTCACTAATAATTTTTGTTACTTCATCTTTTTTGCTGTTGTTATTGTTTGTAAAGTATACTTTGTAACCGCCTTCAAATAACAAGTCTTTATCTTTTTCATTTTTAGCGCGTTCTTTAATTCAAGTTGTTAATTTCTTATTAACATCCAGAGCGTTTTGTTCAGCATCAATTCTGTATTTAATTTCATTCGATTTTTTCAATTCTTCAATATCTTTGTCAGAATATTTAACACCTTTTACTGCTTTGCCTTGTGCATCAAGTGCATAAATATTTTCTTGTTCTTTGAGATATTTAACAAAATCAGCATCTTCTAATAAAACTTTATTTACATAATAATCTGATTTTAAGTTTTTAGAAATTACATCTAATGCATTGTATTTTGTTGCATTAATTGATTTGTATTTTTTGCTTAAAATAAAATCATTTTTTAAGATTTCAAGCATTTTAGCTCTGTTTTTAGCTTGATCAGCATCTGTTAATGATTGACAATAAACTATTGTAGCACCTTTACTATTTAAATAAAGGTAAGCATTATCTAAACCTTTAACTTTTCAGAATGTTCTTATTTTTTTGTCAGCACCTTCAAAAGCTTTAGCAATTAGTGGTATGATTTCTTCTTTTAATTTGTCATCATATAAACCACTCTTCTTGTTGTTATCAAAATCTCTCATGATGTCAAGAAGTTTTTTGTTGTAATCTCCAACTTTTTTAGTTTTTTCTTCTAATGAAGCTGTAGCATCATAATTTGGTTTTAATGGAACTAATTCAGGGAATAGTGTTTCTAATTTAGATTGAATACTTTCAAATAATGCTTTTAAATCAATTTCGGGAACAGAGGTTGATCCATAAACTAATTCTGGCATTGTTATTAAACCTTTAATAACTTCATCATTATTTGATTTTAATAAATTGGTTAGAGAAAGAATTCCCTTAGTTCCAAAATTATTTTTGTATTCATCAGCGTCGGCTGATATTCAACTTAAAAATGTTGTATAGTCAACAATTTCTTGAGGCAAATTAGTTGTACTGTTAGCATCTGTGATAATCTTTGAGTAATGTGAATATGGCTTAAAGTCTGTAAAGATTTTTTCATAAGATTGAATTTTTCCGTGTTTTTCATCAGCTGGGTAGAAATATAATAATTTTCTTAAAGCTGTTCTGTCAATTGTTCATTCTAATTCTTTTTCGCCTTTTTTAGCTATTTTTGTAGGTGCAACACCTGGAATCGTAAATTGTGTGAATGCATATGGATTATCATTTTTATAGTATTCATCAATAAATGCAGATGCATCTTTATATGCTTTAACATAACTATTTGTTGTAAATGACATGTAATCATCTTTAATTTTGAAATAGTTTTCGTTTTCTTTGTAGTAGTTAAAAACTAATTGACCAGTGTCAAATAAAACTTTGCTTGTATCTGGATTTCCATCTTTATCTAATTCATAGATCTTTTGGTTAGCTTTACGATTTACTATTTTATTGATATCAACTGTTGAAAGTTCAAAACGTCTTAAAGCATCTTTTTCAATAGCTTTAAAAACTTGATAGTCAACTGCTTCATCAATGTTTTTAGCTCCATTGTAATTTGAAACTAAAATTTTGTTGAATTTTTCTTCTCATTTATCATAACCGGCTGTTCTTTTGGTGTTTTCTTGAATATCTAGAATCTTGTTTCTTTCTTTTGTTTTAAGTTCTTCAATTGATTTTAATTTGAATGAAGTATTTTCTTCTTCATTGCCTTCACGTGACATATTTCATAATCTTTGATATTCAGCTGAGGCTTTTGCTTCTTTATCATAAAGATAAAACATCGCAAGACGTTTAATTTGTTGTAACAATTTACTTGGATCTTTAGCATCTTCAGTTTTACTTGAATTTTTTAATTCTTTGATTGTAATAGAATGTTTTTCTTTTGATCCTGGTAATGTAAATGTTAAAACAGCACTTTCATCTTTGAGTGCGTCTTTATATGTTACTTTTGTTGAATTAATTACAACTGGAACAATGATACCTGTTGCAATTGCAGATGTTAAAAGTGTTCCAAGGATTATACCTTTTAGCTTTTTCTTTTTAGGCATTGCTTGTTGATTTTTATATTCGTGTTCGGCATTTTTTCTTGTCAAACGTTCGAAAAATGTTTCTTTTCTTTTAGCCATGCTTCCTCCATATATTTAAATATATATTTAAAATTAATATTGATATAAAAATTATAATTTAATTTATTATATAGTAAATGTAAAAAAAACAATGCTTTTTTCAAAGTTTATTTATAATTATTCTTATGACAAAAGATGAGCTTTGGATTGCTTGAAATGAATTTAAAAAACTATTAGAACTTAACAAAATTCCATATTCTTTAAGCCCAATGACAGCAAGAGTAATCTCTAAAAATGAAAAAGTTACACCTGATAACTTTCGTATTTCTTTGTGGTGAAAAGATTTTTTTGACCTCAAATATTTTAATAATTCAATATTTCTAACAAATGATGAAACAAATGAAAAAGATTTAAGTCCATTTTTTAAATGCCAAAATAAAAGAATTTATATTGATTTAATTGTTGGCACAAGTAAAGAAAAATGCAAAAATTTATATAGTTTTAAAACTCATAATAGACTGCTTTTTTGAGGAAAAAATAAGATAAATTTAGCAACAAAAATATTTGGAAGTAAGTCAAAAATATTAGGTTTAATAGAGCTAATTAATACAATTAAAGAAGACAGATTTTTAAGATTAATTGTCTTGAGTTCTTGCCATGACGATTTTAGATTTTTTTCTGATTTAAACTGAAAAACAGTCGAATTGGCAAAAATCAATGATGAATATTTTCCTGTGTTTAGTCAATTTTTAAAAATAAAATAAAAAAATAAATGATTAAAAGAAGTATAGATTTTAATTTCTATATTTTTTTATGTATTTTTGCTTTTTACAAAATATTTGTAAAGAAAAATACTCACAATTATTTGATATTAATTTATAATATAAAAAATTAAAGTTAAATTTGTAGGAGAAAAAAAGTGAAATACAAACGTATATTAGTAAAACTTTCAGGTGAAGGTTTAGTAAATAAACAAAAAAGTTTAGCTATTGACTATGATTTAGTAGAAGACATTGCTAAACAGCTTAAAAAAATTATTGACAAAGGTGTTCAAGTTAGTGTTGTTATTGGTGGAGGTAACTTTTGAAGAGGTGCTTCTGCTGAAAAGAATGGAATACCTAGAAATAGAGCAGACTACATTGGTATGTTGGCAACTATCATGAATGGTCTAGCTCTTAAAAGTGGATTCGAAAAAGTTGGATTAAAAACTAGAATCCAATCATCAATTGCAATTGATCAAAAAGTTGCAGAAAATTATATTAATGAAAAGACTTTAAAATACCTTGAAGAAGGTGAAGTAGTTATTTTTGTTGGCGGTACAGGCCGTCCTTACTTTACAACAGATACAGCAGCAACATTATTTGCTTCAGAAATCAAAGCCGAAGTTATCTTAATGGGTAAAAATGGTGTTAGTGGAATCTATGATTCAGACCCTAAGAAAAATCCAAATGCCAAGAAATATGATGTTGTAACTTATGATGAAATTTTAGACAAAAAATTACAAGTCATGGACTTAACGGCCACAAGTATGGCTAGAGACAACAATATTAATTTAATTGTTTTCAACATTAAAGAAAAGAATGCTATTGTCAAAGCTCTTGAAGGTAAAATTGAACACACAGAGGTAATTAAATAATGGAATTAGATTTATATTTATTAGATTTTTTAGATGAGGCTGAAAAAGCAATTAATCATTATGCTTTTGAATTATCAAAAATTTCAACTGGACGTGCTAACCCTCAATTAATTAAGGGAGTTAGAATTAATTATTATGATACTTTGACACCTCTTGAAGAACTTGCTTCAATTACAGTACCAGAACCACAACAATTATTAATTAAACCTTTTGACGTTTCATGCAACCATGAAATTGCAAAAACAATTTTAAATGCTAACTTAGGTTTAAATCCTGTTGATGAAGGAAATCAAGTTAGAATAACTTTTCCTCCTCTTACAACTGAAAGACGTAAAGATATGGTTAAGAGTCTTGCAAAATTTACAGAAAATGCTAAAGTTGCAATTAGAAATGCAAGACAAAATGTTAACAAATCTATCAAGGCTGATGAAGAATTATCAGAAGATCAAGAAAAGAAATACTTAGATGAAATTCAAAAAGAAGTTGATAAAAAAATCGAACACATTAACAAAATGACAATCAACAAAGAAAAAGAATTAATGAGCATATAAGCCTGTTTTATAGGGCTTTTTTCATTTTTAAGAAAAATTTTGCAGAATTTATTATCTAAATAATAAATTGATATAATTTCATTAAGAAAACGAGGATATATGCTTTTTATTAAGAAACTTAAACCATATTTTGAAAAATACAAAAGATCACAAGACAAAATTCATAATTTGATGCTATTAGATTGGTTGATAGCTCTTCTTTTAGTTTTATCATCTGTTATTATGGGAGTTTTACTTCATTATTTAAATAATTGAAAACTTCCTTTCATTAAAAGTAATAGTTTTGTAATAACAGTTGTTTTGTATTCATTGTATTGTATTTTTGTAGTTGGAATATCTATTTATTTGATATTGAAGAGATTTTTCCTTTATAACTTTTTAATAACATTTGATTACAACTATTTGAAGAGTTACAAATATATGCTTAATAATTTAAAAGTTAATCTTAAATTAAAAGAATATAACGAATTAAAAAGATTAATATTTGATTTAGACACAAATATGAAAGAAACTATCCAATCAGTTACTAATAAAGAAAAGATTACATTATTAGATTCAACAGCTTTAATGTGATTAGATCAAAACTTTTATCGTGAATTAAATAAACTTCACTTTATTAGTTCAGACAAAAAGACTAGAAATCATGTTTTCAAAATTAAAGAATATAAAGCAATAATAAACAAAGAAAATCTTTTTGTGGGGCAAATTAACAATAACCAAGTTGAAATTGAATATGCAAAATTAATACCAACTAGTTTAGTTGAAAAATATCGTGGTGTAAATATACCTAATAGATATTGACTAATGTCATCAAGCATTTTCAAATTATTACAATTTGTAAATAATGAAGAAACACTTAGTGATTCTATTTTATATTTCATATTGAGTGACTTAGCACTTATTTTGTCAAAATGAAAGAAATGAAATATTGTTAAACTAAAAGAAGTGTTTGAGTTGTTTTTAATTTCAAATATGTTTGCAGCTTTTTATGATCGTAGTGAATTCTTAAATTATTCAATTAGAATGCTTGATATTTATGAAGGAACAAAACTCAAAAACATTTTTACAGCTTTGGAAAACAATGCAAAATTTGGTTTAAAATCACTTAACCTATTTTACGAAAAGGTCATTAAAAAAGTTGTTTTCGACAAAAAAATAGTTGATTTATCACAATCAATTAAAAACACATTATTAGGTCGTGAACTAATACTTAATGAGTATTTTTCATCAACATCACTTAAATCATTTGACAATCATGCAATGTTTTTAAAATTTAAAGACGCAAAAGAAATGAATAAATATTTAAAGAAAATTGAACCAACTAAAATTCATTTTGAAACAAGCAAAGTATTCTTAAATTATCTTTCTCCAAAGGTAAATACAAACAAAGTTGACATTCGTTTTGTTTTAACTAAATTAGTTAACAATTATGTCTAAAAGCAAACAAAAAATAGGTATTATCAGCCTATTATTTTTTTACTTTTAAACTTTTTAAAAAATTTTTAAAATCATCAATTAATTTTTGAAAATCAGCTTTTGATTTATTTCCTATTATTTCTTTTCTAGTGTCTTTTACAAATTTAGCTAAATCCAATCATTGTTGGTTTGTTCCATTTATTTCTTTCAAAGTTGTTTCACCTGTTACAATAAGTCCTTCTAAAGTAGCTAAAGCTATTTTGTCTCCAATATTGCCTTTTTCTCTAGCTCTTTTATACTTAGAGTATTCAATTTTATAAACAGTTTTTCCATGCAATGTACACACAAGAGCAGCGGTTACTGTGCTAGCTACTAAAATTGTAGCTCCTACTGATATACCAATAATAGCACCTTTACTCATCTTTTTTGCCATAATACACCTCCGAAATGTATATAATAATTTTACTATTTTAACAACTAAATATAAAAATAATTCTTTTACTTAGCTTTATTAATTAAATAAATATAATTAAAATATGAAAAAATACGTCAATTTATACAATACAAGTGAATACACTTTTTTAGATTCTTTAATTAGAATAGATGATTTAGTTAGATTATCAAAAGAACACAATCTTCCTGCAGTAGTTTTAAGTGACCGAAACAATATGTTCGGTTTAGGTGCTTTTATGGAAGCATGCTTAAAATATGATATCAAAGGTATCATTGGAGTGGACTTAGATGTAGACAATTATCGCTTCATAGTCTTAGCGAAAAACTACCAAGGTTACCAAAAAATTAATCAACTAATTTATCAAAAAAGTATTAATAAAGCTATTGAAATTAGTGAATTATGAAATCCCAATTTAATAGTTTTAGATCATCCAAGGTATGGTTACTATGCTCAAACTAAAGATAATAAATATAGTGCAAGTAAAAACTACTATATTAATTCTTCAGATGCTTCATTACCTAACTCTATATTAATAAAAGAAAATAAACTTTTTGAAGCTAGTGATAATGAAACTCTAAATTACTTACAAAAATTAGGCAATTTAGAAGAAACTAAACACTATGCCAACTATTTTGATGAAGTTAATGTAGATAGCGAAATAATTGACCGTATTTATTCAATAATTAATGAATGCAATGTTATTTTTCCTAAAAAGGCCTTGCATTTAGCTCCTTTTCAAAATAATGATGAAGCTACCAATGAAAAGCTTTTTGTTTCACTTTTAAAAGCAGGCGCTAAAAGACTAGCAAGTGAATTAAAAAATTATCCAGACTGAAACAAAAGACTTGAATATGAGTTTAATGTCATTAAAAAACTTGGTTATATTAACTACTTTTTAATTATTCAAGATTTAGTTAACTGAGCTAAAAGTCAAAAAATAGCAATAGGACCTGGTCGTGGTAGTGCAGCAGGTTCACTTGTTTCTTATCTTTTAAATATAACTGAAATTAATCCATTAAGATATAACTTACTTTTTGAAAGATTTTTAAACCCTGATAGAGTTTCATGGCCTGACATTGATATAGATATTCAAGATGATCGTCGTTTTGAAGTTTTTGAATATTTACAAAAAAAATATGGAGTAGATAAAACTGGACTTATTTCAACTTTTCAAACTATTGGTGCTAAAATGGCAATTAGAGATATGGGCAGAGTTATGGGAATTAGCTTAATTGACATCAACCGCATCAGTAAAACTATTAAATCAGATAACTTAACTGATGCTTATAAAAATGATATTGACTTTAAAATTGAAATGGATAATTATCCTGAGTTATATAAACATGCACTTAAAATCGAAGGCTTACCTCGTCAACAAAGTTTCCATGCAGCAGGTTTTATTATTGCTAAGGACAAATTATTTAATTATGTCCCTGTTTGTTTAAGCAATTTAGACAATTACCAACAGGTACAAGTACCAATGAATTACATAGAGAGTTATGGACTTTTAAAAATTGACTTGCTAGGTTTAAGAACTTTAACTGAAATTCAGCTAATTGAAAAAAGTTTAGAAGAAAACCAAAAATTTGATTATTTAGTAAAAAACGATCCTCAAATTTTAAATGATCCTAAAACTTTAACAATGCTCAACTTAGGTTTTACTGAAGGAATATTTCAATTAGAATCTCCGGGCATGAAGAAGACAATTCAAAAAGTTGTCTTGAATTCTTTTGATGATTTATATGCCATTATTTCATTATTTAGACCTGGACCTATGGACTTTATCAATGACTATGCAACTAATAAGAAAAATCAAAAATTAATTAAAAAAATACATCCTGATTATGACAAAGTTGTTGCCAACACATATGGCATAATAGTTTATCAAGAACAAATTATGCAAATAGCTCAAAGTATTGGCGGAATGACTTTTGCTCAAGCTGATTTATTGCGTAAAGCAATTAGCAAAAAAAGTGAAGTTGATATCAAAAAATATAGAAGCATATTTTTTGAAAATGCCATTAAAAAAGGCATAAACGAAAAAATTATTAATGCTATTTATAACAATATTGAAAAATTTGGACTATACGGTTTTAATAAATCTCATGCTGTTAGTTATGCATATTTAACTATGAAAATGTCATACTATAAAGCTCGTTATGCTAACTTATTTTATAGCTCTTTAATTACAAATGCAGCTGGAGCACAAGCTACAATTAATAAATATGTAGCTGAAATTAAGAAACTTAATTTTCCAGTTTATTCGCCAAATATTTTGCATTCAAGTAATAAATGTATAGTAACAAACGATGGCATTTATTTACCTTTTGAAATGATTAAAGGTTTTGGAGCTGAAGGTGTTAATAAAATTGTGGCTGATTTAAATGAAAATGGACCATTTCAAAAGAATTTAATTGATACAATGCTTAGACTTCGTTTTGCAGGTATTAAAGATGCTGCTATGGAAATATTAATTAAAGCCAATGCTTTTAGAGACTTTGGACACATGAAATATATACTATCTTGTGATAATCAAATTAAAGACATTTTTAAGGTTTTATCTTCACATTCAACTTATAAAGAAGCATTAAAAGATATTCAAAAAATGGGTTACTATGATATAGTTTTTGATCAAGAAAATGAACGTGACATAAATTACGAAATGGACTATGAAATAGCTCTTTTAGGTGAATTATACAATGCTTTTAGTACTTCAATTTATGAAGCAAAATACAAAAATAAATTAAATACAATAACCTCTTCAATTCCTGTTTCTGTTGCTGCTGAAGTAGCTGATTTAAGAAGACCAAGAGGTAAAGAATTTAGCGTTTTAGAATTAAGAGATAGTACAGCTAAAATTACTTTCTTTGTTAACAACAAAGTATTGGCAAGCTCACTTCCTCTTAAAAAAGGACAAATAATTGAAGCTACAATTAGTTTCAGTCGCAACAAACATAATTTAATTAGCTGAAAAGGAATTCAATAATGAACAAAGACAACTTTTTATTAATAGATGGGAATTATTTATTATTTTCTTCTTTCTATGCTTCATATAATCCGGATAGATTAGAAAATATTATGCGTTCACCACAAGGTTTAACAACTAATGGTGTTCATGTTTTTCTTATGACTTTAAGTAAACTAATAGCATACTTTAAACCTAAATATTTATTTATTGCTTTTGATGCTTATGGAGAAACTAGAAGACATAAAATATTCAAAGATTATAAAGCAGGTAGAACCAAAGCACCAGAAATAATTTTTGAACAATTTAGCTTAATTAAAGAAATTTTGACAAAATTAAATATCAAATGATTTGAACAAGTTGGTGATGAAGCGGATGATTTAATTGCAACTTTAGCTCAAAATCAAGATTGCAATAATTTAATTTTTTCTAAAGACAAAGACCTTTTACAATTAGTTAATCTTAATACTAGTGTAATTAAAGTAGTTAAAGAAAATTTCAAAGTTTCTTATGAATTAGAAGACATTAACAATTTTGAAACTATTCACGGTATTAAACCAAGTCAAGTAGTTGATTATAAAGGTTTAGCAGGAGATTCTTCTGACAATTTAAGAGGAATTAATGGCATTGGACACAAAGGTGCAATTAAATTAATTCAGACTTTTGATTCAATTGAAAACATGTATCAAAATATTGATCAAATTAAAGGCAAAACTAAAGAAAAATTATTAGATGAAAAAGATCAAGCTTTTTTATGTAAAGCATTAGCAAAATTAAATTTAAATGTTGATATGAATAAAAACATTTCTGATTACCTATTGAACTTAAACTTAGAAGATGGCTTAGATGCAATGCGTTTTTATGGTTTAAATAAATGCGCAAATTTATTTGAAGAATTGAAATAAAAAAAGAATATCAATAAGATACTCTTAAAAATGAGGAATAGCTTTATGCTATTTTATTTTTGTTATATTATATGTTAAATAATTACCATCGTAAAAGTAAGGTACTTTGTCGGGAATTAATGTGTATGTTCCATTATTGCATTCAAAAATAAAACTTAATTTATTATATTCCATATTTGGATAAGTATTAACTGGAACATTGAAATAATCTGCATGATTTTTAAAATATCAAACTTCTTCTCCATTATTATTTGTTGTTTTTTCCATATTTTTTTCTTTGAAAATTTCAAAATCATCTTTTAAACATCTGATTGTTTTAAATCCCATCAAACTTTTCTCTGTTAATTCAAAATTAAATGTATTAATATCTAAAGTGTCCATATAATTTTCAGCTCTAAACAAGTCCATATTTTTTGTAATATTGTAAATAGGATTTAATGTTTTTGATTCCATATAATCTATTTTTATAGTAAGTCTAATTTTTTTGTTTTCTCATTCGCCATTATCTTTTAAGAAAATACCTGCGTCATTTAAAACTTCAAATTTTTTGGAACTATCAAAGCTATAGATTTCAATTTGTTTGTCTTCATCTAATCGTCAATTTCTGCCAAAAAATAATTTAAAATTGTCTGATGTAAAACCGATATTTTCTCAAAATTTAATATTTACAAAAGAACCAGGGTAAATTTGTCCTTCAATTTCTTTTATTTTTTTAGGAAAGATAATCTTTTTAGTTTTGAAAAATGATTGCCCTCAGATACCATAATCATCAAAAGATAAACATTTTATTAAACTTGATTCTGATAAGTCAAGAGTATCTTTACATGCGCCTAAAATGGTATCTAAATTGCCCATCCATAAAGTACTAAAATCAGATAATGTTTCTTCTCTTAAATTAATTTTAAATGAATCTGGCATTCTATTTATTTTATTTAATTTTTGGAATAGAAAAACTTTATTTTTCGATTTCGAACAATCTAAATTTTTTAATACATTTTGAACGCCTTTTATGTCATTAAAATCTATATTTTTAAACGTTTCTTCTATATGAACATTATTCAGAAAGTCATCACCAAATTCTATAGTATTAACTTCAAGTTTTTTGGCTATAATGTATTCGTTTTCAAAGTCTGGTTTAAAATTATTTAATGTTAAATTATTTATTTTTACATTTTGAAATTGATTTGCTTCACGATAATTAAAATTATTTTTTTCAACAATAATATTTTGTTTCTCTGCTACAAAATTATTTAAATAGAAATAAATCCCTGACATGTAATTTTTAGAATATTTTGAATTGTTGAATGAGCTAATTAAAACATTATTAAATTCTGATAATTTTATATTAATTCTTTTATAGAAATAGAATGTATCTCGAGTGCTGTAGTCAAAGTTGACATTGCAGTCTTTCAACAAAGAAAGTCTTAACATGCTTTTCATAAATTTAGGATTTTGCAAATCATTAGTTTCTAAAGAAAAGAAATTTGAAGAAATACAAAAATTTTTAAAAGAAATAACATCACTTTTATTTTCTCTTATTTTAATTTCATTTTCTATTTCTTTTTTGACTTTTTTCAAGTTTTCTTCATCACTTAGTGTCTCATTTCTTTTAGAATATTTTTGAATTGCCTTTTTTTCATTATTGTTAATTCAATCTAGTTCTTCTTGAAGATCTTTTTTTGTTGAAAATATATAACTTGAAACTAAAGCAATGTCATCTTCTTTTTGTAAAACTGACAATTTATCTAGTACTGGAAAATAAAAATTATCAGATTTTAAATTGTTATTATCATCACAATATCATTTTTTTACAACATCATAGAAAAAATCATAATCAATATATTTTACTAATTTATGTGTTTTAGAGTTTTCAATTCATTTTGTATTTTTTTCAACTATTTTATTCAATTCTTTTTTGTCATAATCTGTGCAAGATCTGGGTAATGGGAATGACTTTTTTTCTTCTGGCTTACTACCACAAGATGTTATAGGAGTTATAATTAATGGAGTTAATAATAAACTATTAAAAATTTTATTTTTTTTCATTTTAAACCTCTTTTATTAAGAATATAACTTATAAACATAATTTACTCCTTTTTTATAATTTTAAAAGCAAAAAATATAGATGAAAACACAAAATTTTATTTTTAAAAAATATTTGTTTTTATTTTATTTTGCTTTATAATTAAAGAGCATTTTAGCAAATGCCCAGGTGGCGGAATAGGTAGACGCAAGGGACTTAAAATCCCTCGAACGTTAGTTCGTGCTGGTTCAAGTCCAGTTCTGGGTACCAGTTGTCTACTTCGTTAGGCATCAAATATGCGTCCTTAGCTCAGGCGGTAGAGCAAATGGCTTTTAACCATTGGGTCGGAGGTTCGAGTCCTCTAGGACGTACCAGTTCAAGTAATTGACCAAGCATCCTTTCAATAGGATGCTTTTATTTTTGCAAAGTAAAAAATATCAAGCTCAATAATAAGCTTGATACCTAGATTGTAATTTGTAAGTTTACACCTAGAAATTTGGAATATCGCATGTCGATATTCCTTTTTAATTATTAATATTGTTATTATTTTTTGTCCGTCAGGACAAATAAAAGTGAGGGCATTAGTCCTCCCTAAAATATTTGGCGACTTGTCGCCCTTTCTAAAATATTATTTTAATTTTTTTATTTTTTTGAAAAACAAATTTCTATTCTTGTTACTTTCTCAGTTTCTCTTTTGTTTTCCATATAATACTTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCGTACATTTCCAAAGCAGATAAATATCCAAAAATTTCTCTTGGACGATTGTTTATTTCATTTTGTACTTTTAGAATTTCTTCTTCAGTAATATCATCAAAATTAGTTTTTTTCTTAAAAAATCTTCTAACTAAACCATTGAAATTTTCATTTGAACCCCTTTGAAAAGAAGCGCATGGATCAGCTTTATATATAAAAATTTTTAGTTTATATCCAATGAAAAATAATGTTGAAAATTCTAATCCATTATCCTGAGTTATAGATTTAACATTTAATTTATGTTTTCAAATAAGATTCCATAAATACAAATTTAAATTTCATGGATTTTTATTAGGAATTTTTACCAAAAACCCATATCTAGAGACTCTTTCGGTAAAACTAAGTAAATGACAATGTGTTCCTTTGCTCTTACCTACAATAAGGTCTATTTCCCAATGGCCAAATTCATTTCTTTCATTTATGTTTGCTGGTCTAGATCAAAAAGGTCTTACATAACGTGCACCAACCAGTCTTCTAGCGGCGCTTTGTTTCTCTCTTTTTCCACCTTTTTTGTAGACTTTTCTTAATAAACTTTTTCAGTTCAAAATTCAATAACCACTTTTTATTCAATTGTAGACTGTTTTAAAAGAAGGAACATCAATATTAGGATAAAGATTCTTTATTTTTTCAATTGTTAATTTGACAGTCCACATTTTGCCATCATATTCTTTTTTAAATAAAGAAGAAAATTCTTTATATTTATTTAAATTGTTTAAAAATTTAAAATGATTTTTTCACTTTTCTCTTGTAATTATTTTTTCAATTGCATCTAAAGCAAAATAACCCAAAAAAGTTGAATTATTTTTTATTTCATTATTAATAGTTGTTCTTGAATAACCTAATATTTTTGCTATTTTTGTTATACTATAATTAGTTGCTCAAAGTAAGTATTCAAGAACAGTGCGTTCATTTCATGATAATTTTTTTTGCATTTTTTCATGACAGTCGCCAAATACTCTTTGGGTGACTTTTTTTATAAAAAAAACTTCACCCATTATAGATTTTATCTAAAATGTGTAAAGTTTCAGATTACAATGTAGCTATCAAGCTAAATAATAAGCTTGATATTAATATTGATTTATTAGTTTTCGTTGTAAATATCTGCAATTGTACGTTCAGCTTGATATTTAGGATTAGCTAATATTTTCTTCAATTCATCATAGATGTTTTTGTAAGTTTCACTGTATCCGAATGAATCTAATCATCCACCATATTCACTATTTCCTGGAACCATACTGTAACCTTGTCAGTATGTTGTTTTTTGAAATTCTCCATATTTACCATCTGTTTTTTTAACAAATCTTAATCCTGGAGCACCATTAATAATCTGTGAATAATTTTCTCATGTCTGTGTTTTTAACGTATTGTTGAGTTCTTTTGTCATTGTCTTTACCTTTTGAGTTTCTATCAAAGAAGTCATTAAAGTTGTCGCCTATTCTCATAAGTGTTCTCAATCTAACAGCATCACCTGATTTAGAATCAACAGTTTTTAAGTTTCATCCTGTTGCATTGTCGTCTAAACCATTCATACGGTCTGTTTTGTAAACTTTTTTGCCTAATACATAAGCTGCATTTGAATTTGTTCATGTTCTTCATGGTGTAGTTTCAAAAGTTTTTGTAAATTTCATTCTTTCAGCTAGGCTTTTAAGATCACCCTTAGACATATCTTTTGTCATTTCGTCTGTAATAACTTTAGTTCCATCCATTGCTACATAAGGCATTGAACCACGCATTCAGAATTGGAAGTCTGAAACATATTTAACACCTAAGTTTATCAAATTCTTTTTAACAGCTTCTCTAACTGCTGTAGATTGGTTCATATCTGAGTTATAAACAACTAAAGCACCTTTTGATTGTACATAGTTAATGAAATCAATAGCGCCTGGCACAGCAAATCTATTTCCTTTAAGGTCGTTCTTTTCTTTCGTGTCTCCATTGTAACCACCATTTAACATACCAAATGTTTCTGTGTAGTCATTTTGTAAAACAGTTTCATCAATGTCCATAAAGATAACTGGAATGTATTTATCTGTTGCAGCATTATTTACTTGTTTAGTTGTATTAAAGTTTTGACCATATTCAAAACCATCGGTTACAGCATATTTCATTAAGTTGTCAAAAACAGCTTTTGCTCTAGAATATTCAGCTTCTTTTGCAATTCTTGCTTCAACTGATTTAATGTATCAAACAATTGATCCAAATTGAGCAGCATCTTTATTTAATTTAGAAATTAATTTTTCTTTTTCATCCATTGACATAATTCCTTTGTAGTCAATGGCATTAATAAAGGCTAATTTTCCTTCATTATTTAATTTAGCGAATTGTTCTTCAATGGTTGGTTGTTTTCCACAACTAACTGCAATAGCAATAGGGCTTAAAGCAACAACTGGAGCTAAAGCACCTAATAAAAATCTTTTCTTCATTAGTTCTCCTCTATTTTTTTATTCTTTTAATTAATATTGAAAAAAGGCTAAAAAAATAAGCAACCAAGCTCAATATTAACATTACAATTATATTAAAATAAGACTTCAAAAGTGCTTTTATGAAAAAAATGACACATAAAAAAGGAGCTTTAGCCCCTATATTTAATTGTAATTAAACAACTTTAAGAATTTCTTCTTTTGATTTATGTTTACCTTTTAAAGAATCCCTTTCAACATGTATAATTTGGATTTTTTCGTTGGCTCTTGTGTGTTCGTCATATTTTTGTTTACGACGGAAGCCAAGGATTAATCCTTCGATTTCTTCAAGTGAGTTTGCTTGTGCAACAACTTCTTGAGTTGAAACTAATTTTGCTTTAAATTTATACATAAATCTCCTTTTGTGGTTTATTGTAACCACCTATTTTTATTATATCAAGTATGAAGTACCAAAAATTAGTTTGTGCAAAATTTAACACTTATTTATGGAATTGTTATTACTTTTGGTTAAATTGTTGAATATTATTTTCAAAAATTATAAAAATATGGTATTTAATCATTTATAATAAAAATGTTAGTTAAATATACTAATATGTAAGTTTTTTATGAAAGGTTATCATGATCCCACCTCAAAATATTAAGCGATCAGGATCAAAAAAGCGCAACAATGCATTATTTTGAACATTAATATCATTGTCAATCTTTTTGTGTTTTGTTTCATTGTTTGTTGCTGTTTATTTACTTTTAAATAATAAAAATGAAACAATAAATAAAAATAATAATTTAGTTAAAATAAAAATACAAAGTAAAGAAGATAAAATAGGACTTATAAATCAAGATAAATATATTGAACAATATGCAAAAAGTCCTTCTTCATTAAATAATTTTGACAATGTTACAAATGATTTAAAAAATCAATTTATTGATTTAAAATTTAATGATTATATTTTGGCTAATAACACTGTTTTAAAGTATGATACGAAAAAACATAGCTATAAATATGATAAGAGAAATTGAGTTAAAAATGGAACGTTTAAATTTGAAAATAACAGTTGAGTATATATTGATCCTATTAACAATGTCAAATTTATTGACAAATCTTATGGCGTTGATAAAAACAAAAAACCACGCTTCCTTTTAGGCCCTTATGGTTTAGCTTTATTAGCTAATTATTTCTACTCAAGAATGACTTATGGACCGGAAATAAAATTTTTAGATTCTATAAACGTTAATGACTTTAGTATTATCACTAAAGAATCTGCAGGTATTTATTTGCCAACAATTCAAAGAATTTATTTAAATGGTGCGGCACTAAGTGAAAAAGGAGCAAAATTAAGTGTCAAAGTAAAACAAATACTTTATTCACTTTTTCATGAATACATGCACCATTGAGCACACAGTTATGCTTCATATGGTTCAATTGAAGATGTTAAAAATAATAAAGCAATTACAATTCCTTATCGTTCAAATTTAGAATTTTCAGTAAAAAGTGATTTTTGATATAAAAAATTTGCTAATCAATTTAAGGAATTATTAAATTATGATGATACATGAATAAACTCAATAAATCCATGGTTTCCAGGAGACAATTCATTTGTTCCTTATAAACTAAGTCTTAATGACTTGTGAAGTTTATCAAATGAAAACGATAATAAAAAATTCAAAGAAGCTTTAAATAAATTGAAATCTATTAAAGATGAAAATGACAAAGTAACTTTTAGAGAAACTAAAGATTTCGATTCAAAATATTTAATAGCCGATTATTCAATAAATAGTTTGCCTTATTATTATTCTCTAGTTGAACTAGTGCCAAGAGAATGACAAAAATATGCTTACATACCATTTAATGATCCATCAAATCCTTATAATTATTTATATGAAAAAATAATTAAATCAGATACTATAGATGATTTAGATATAATTGAATCTTTTTATGGTGTTACTTTTAAAGATTCAAATAATTTGTTGACCTATGCCAATAGTTATTCAATTGATTGATCAAGAACAATGCATAAAGCTCAATTAGAAGCTGATCGAAACTTTTATAGTAACAATATTTATAAAAGTGAAAATCAACAACTATTTTACAAATTGTTTCTTGAAACAATGGGTTATGGTAAAACTATAGCTCAAGTTAAAAGCAAAATAGATGTTAAAAAATTAATTGCTGGAGACACTGATAGTTATTCAGTTGAAATTGATAAAAATAATATTTTTCAAACAAGAATTAGTGGTTATTTAGATAACAAAAATATCAAAGGATTTTATTTCACAAATAATAAAGATGAAAAAGAGTTTGTTAAAATAAATTATTTAAATGCATTTAAATTTGAGGGAAAAGATAATTTATTAGATACTACTTTTACCTTATCGCCAAATATGGTTTTAAGTGATTTAATTTCAAAACAAGCAGCTTATACAAGTGATTATTTTGATTATAGAAATATAAAAGAAAATTCACCTATTGAATTCTGACAAGATCTAAATAATGATGGTGAAATGCAAGATAATGAATTAATCAAAGAATATGAAAAAGATCCATTGCCAGATAGATATTTAACTAGTAGTGATGCACCTAGTGAATTAGATTCAACGAATTCATATAAAATAGAAGCTAGCTTTATAAGCAAAAAACAATCAAATAAAAAATATGGAATATATTTTAAAAGGAGGGAAATGAATGTCTTTAGTTAATAAGGATAATCTTCTTATTAAAGAAGAAAATTTAAAACCTAAAAAAATAGTTTTTCCCTTAATTGGATCAATTATTAATGTCCAAGCTTTTAAATTTAATGGTGAATTATACCGCCAATGAAACGGTGTTAAGGTTTTAAGAAATACACCGCAACATTATGTTCTATTTATGTATAAAACTAAAGTAGCTGAAACTGATAAAAAAGGTTGAGTATATCGCGAATATGTTTTATGATTTATGCCTAAAAATAGTATGTATAATGCCTTGATTTTATTAAAACCAAAAAGTAATTATGTCTATGTAAATTTAGCTTCAAAACCATATTATGAAGATAATACAATCAAATTTGTTGATTTTGATTTAGATGTTAAAAGCTATCCTAAAAAAGATTTTACAATTGTAGATAAAGAAGAATTTCGTTACAATTCTAGACGCCTCAAATATCCTAAAAAACTGATAAATATGATTTACGATAATTTAGAGGAAATAGTTAATAAACATAATAATGGTGAGTATTTCTTTAATCCAGATGTAATTGATTATTACATCAATCAAGCTAAAAAAGACAAATCAATTTATCGTGATTTTAGAGTTAAAAAAGCAAGTAGTTATAGTTTTAAATGAAGAAATAAAAATAAATAGAGGTTAATTGAGACTGTTTAGAATTTTGTGTAAAGTCTCTCCTTTTTTCAATAAAAAGGAATTAACTTTACATCAACACCTAATAATATTGCAGAACAAAATAAGATAATGCCTAAAAAACATTATCTTATTTTTAAAATTTGAATTTTAAAATAGGAAAAAGTTCTTGCCATGCAAGAAATCTTAATCCCTCTCCCGTCTCCCTTAACTCATTTAAGGGAGAGGCCTTCTTTGAAGCCAAAGAAGCAAAGCTTTTAAAATTTGAAAAAATTATTCTTCATTTTCAAAATTAATAATTTTAAATTCATTTAAAATGCTAGATCAATTTCTAATTCTTGAATTACTTCATTTTTTATGATTTGTTGAGATGCTAGAAAAAGAATTTTTAAAAGACTATCATCAGATGGGAAAACCGTTTTTGTTTTTGTATATTTTCTAAATACGCTATTTAAATTTTCGATTGAATTAGTGGTATAAATGAGCGTTCTTATTTCAAGAGAATAATCAAACATTGTAGAAATTTCAGATCAGTTTCGTCTTCATATTGAAATAGATGTAGGATATTTTTTGCCTCATTTATCTTCAAAAATATCCAAATTATCCATTGCATTTTTTGCATCATTAGATTGATATACAGATTTTAAATCTTTGCAAAATTCTTTCATATCCTTGTAGTTAACATATTTAACAGAATTTCTAATCATATGTACAATGCAACGTTGTTGCTTACTATCAGGAAAAACGGCTTCTATAGCTTGTTGTATACCTTTTAAATTATCTGAACAAATAATATTAATTTTACTAATTCCTCTATTTTTAAGAGCATTTAGGACAGACATTCAAAACTTTGCAGATTCATTTTCTCCAATATAAAGTCCTAGAATTTCTTTTTGTCCTTCACCATTAATACCCATAATAACATATGTAGCTTTCTTAGTTACAACATTATTGTCTACAACATGAAAATGTACTGCATCAATATAAATTATTGGATAATATGTTTCAAGTTTTCTTTCTTGTCATAATCTTGCGTCTTCAACAATTTCATTAGTTAATTGGCTTATTTGTGTTGGAGAATAATTAACGCCATATTTGCTAAACATAAAATCACTTACATCTCTTGTTGACATTCCTCTAGAATATAAAGCAAATACCATATCAACTAATTCAGAAATATCACGTTCATACTTTTTGATGATTATAGGTTCAAATTTCGCTTCACGATCTCTTGGAATGTCAATTTCCATTTGACCATATTGACTATTAACATTTTTCTTAGAAAAACCGTTTCTATAATTTTCTTTTTGAACTTTATTATCATTTTTTTCATAACCCATAAAATTATCAAATTCTTTGTTCATCATTTTTTGGATTGTATTTTGAAATACTTCTTTCATAGCCATACTAAGATCACCAGAAGTAACTGGATTATAATTTTCTAAAATTTTTTCTGTTATTTCATCAATAACAGGATTTATTGGTTTTCTTTTCATAAATAAAAAATCCTTTCTTGATAATATTTTATTTATTGAGAAAGGATTTTAGAACTTTACACAAAATTTTTTACAGTCCCATTATTTAATTTTTTGAATATTGTAAGTTAAATAATTTCTTGATGAAAATTCACCATTTTTTGCAAAATAAGGATCTTCATCTGGTTTCAACAAATAAGTTCCATCTTTACATTCAAATGTAAAGCTTAATTCGTTATATTTCATTCTTCTGTATTCATAAACTGGAACATTAAAGTAATCAGCATGATTTTTGAAAAATCACTTTTCTTTTCCTTCGTGATTTACTTTTGTCATATTTAACGATTTAAATACATCAAAATTATCATTTAAGCATCTAACAGTTTTAAAGCCCATTAAACTTTTTTCAGCAAATGTAAAATTTAATGTGTTAACGTCCAGAATATCTTTGTAATGACTTGCATCAAAATATTCCTTGCCGAGTGTGATATTATAAATTGGATTTAACGTTGAAGCTGTTAAATTATTTATTTTTATAGTCAATACATAAGGTTTATTTCATTTGTTACCATTAGGTCAAAGAACAAACGCGTTGTTTAACATTTCAAATGGTTTTGAGACATTTTCAAATTCTTCAAATTCAAGTGTTTTGCAATCATCTGTATTCATTCGCTCGTCAAAGAAATAAATAAAATTGTCTGCATTAAATCCAACATTTTTTCAAAATTTAATATTTTTAAATCTATGCGCAGCTATTGCCCCCTCAACCTTTTCAACTTTTTTAGGATAAATTACTTTATCTGTATAACAGTTAAATGCATCATTATCGGTTTGACTATTTTTAGGGTTTCCAAAATATAAAATATTTACAGGATCAGAATCGGATAAATCTACTAAATCTAAATTTAAATTAAATAAACGATAAAGACAATTAACGTATAAATCATTTTCTTCTGGTGGAAACGAATAATCTTTTAATTTAATCTTGAAAGATTTAGGAATTTTATTATTTTTCAATAATTTTATAAATAAATCAGAGTTTCCTCCACTTAATTCCGGAACATATAAGTTTTTAATTAAATATTCTGCACCTTCTATGTCCTCGAAATCTGTGTTTTTTAAGGATAATTTGAAAGATGTTTTAAATGATAAATCCTTATCAATTATAAGTTTTTTTACTTTTAATTTTTTTGCAATATTTAAATAATCTAAATTTAAACCACCTGTATTAAAACTATATTTAAAATTTCTTAAATATAGATTTCTTATTTTTACATTATTAAATTGTTCATAACTTTCCATATTAAATATATTTTTATCAACAATAATATCTTTTTCTTCGTCTATAAAATTATTTAAATAAAAATAGATGTTTTTATTCCAAGACAAACCATCTTTAGAATTGTCTAAATAATTTAATTTAACATTATTAAATTCACTTAAATTAATTCTTCCCTTAAGCTCTCAACTACCAAAATTTCTATCATGAAAAAAAATACTAGAACATTTGGAATCAAATACAAATTCGCAATTATTTAACAAAGAAATTCTAGAAAATTCTCTTTTTCAATCAAAATTATCATTTGAATTCATAGTTAAAAAGTTTGACGGCATTACTATTTTTTTGAATTTTTTTATATCTTTTTTGTTTTTTCTTAATTCGATAGCTTTTTCTATTTCTGATTTAATTTTTTTTAAATTTTCGTCTTCACTTAATTTAAAATTTCTATTTGAATATTTTATTTTAGCTTCTTCTTCATTGTTGTTTATTCAATTTAATTCACTTATCAAATCATCTTTAGATGAAAATAAATAACTAGAAAATAATTTAACATTATCATTAGTATATTCTGGATGTATTGATATTGCATTAAAAGCAGGAAAATAAAAAACATCTGTTAATTGATTTCCATTTTTTTCAATATATCATTTTTGTATAACGTCATAAACGAAATCATAATCAATATATTTTTCTTTTTTATTTGTATTAACATTTTTTACTAATTTAGTATTTTTTTCTAATATATTTTTTAATTCATCTTTGTCATAATCTACGCAAGAAGTCGGAACATGATATTCTTTTACTTTTTTTTCCTCTTGTTCCTCTTGTTTTTTAGGCTTATCGGTATTTGAACATGAATTCAAAACCATTGGTATTGGTGATAAAATACTTAAATTTCTTATTATTTTAATAATTTTTTTCATTTGAAATCCTTTCTAATTTATTTTTATATTTTGTAAAACAAAATGTTTAAAAAAAGTTAAAATGTCGTTGTATGTTAAGAAATCATATTTTTTGTCATTTATTTTAAATCTTATCATATATTTCTTTATTAAAATTTTCATAAATGCTAGATGATTCTTTTACTGAGTTTATTTTTGATTCAAATACTATTTCCTTTTCGTTCAAAATAGTAATGTAATCAATTATTTCATTATTTGAATCTATAACGTGAGATGCTAAAATTATTGACACGCCCTTTTGCTTTAAAGCTTGAAGTCTTTGATAAAGTTGATTTCTTGCATAAGGATCCAAATTTGCTTCAGGTTCGTCTAAAAGTAAAACCTTAGGTTCGATCATATTGATTAGTAAAAACAATAATGTTCTTTTTTGCCCACTAGACAATTTATTTGGGTTTTTATTTATGCATTCAAATAAACCGAATTCTTTAAATTTATTTTCAATTTCAAATTTGCCAAAAATTTTTGTTGAAAAAAGCTTTAAATAAATTTTTATAAACTTATAAGAAGTTAAAAAAACTGGAAAATTAAAATCTGTTGGTAAGAAAAAAATATTTTTTCATTCTTTACAATTTTTTATGTTTTCGTTTTCGTAAAATATTTCTCCAGTAAAATTATCGTCTTGCATTGAAGCAATATTAAGTAGAAGAGTTTTTCCTTGACCATTTCTGCCTAATAATAAATTTATTTGGAAGTGATAGAAAGAAGTGTGTTGAACACTTTTTTTATTTCACTTTTGATTATTTATAAAAGTACTCATAAACATTAATTCATATTTTTATTTAGTTTCGTATTCTGCTAAATTAATATACTTTTCAAAAAAATGATCAAAAGTATAAAAAAATAACAGCGTCTAGAACACTTACTACCCGTTCGACCTGTTGTATATATTATACCACAGTCGCTGTTATATGTTCAAAAAAAAGGAGAAATTATGAGTGTATACGGCGAACTAAGGTTAATTGCAAATGAAGGAAGCGAAAACGAAGTTAGAAAGTTTGAAGCTAACTTGGAATGGAGAAAAAATTATTTTGGCAAGAAGGTTTATGACAAGTTAAGCCAAGATACAGTTAGTGAAATTTTAAAAACAAAGATAGTACTCGGTGAAAGCTACTATAAGATTTTGAGAACTGAAAAAGTTGCTTTTGAAGTGTATGTCTGTTTAAGGTTTCTTGGGGCTAAAAAACGCTATGTAAATTTTTATGAATTAGTGGCTTTTGGCTTCAAGAAAACAAGTTTGCAAAGAGCGGTAAAGTTTTTAACAGATATAGGGCTAATTTTAAAAGTTAGAAACGCTGTGAAAATTAAGAAGTTTAAGCTAACTAATGACGATAGAAAGTTCATTGTTATTAGTGGTTATAAAGACTGGAAGATTTTCTTGTTGTTTGGATTAGCTAATTTATGAGCTTATAAAACGCTTGTATGAAAATCAAAAGAACTTGGAACTAAAAAGTTTGTTAAAAGTAGAAAGATGAAAGTCATTGTACAAAATAACTTTTTAGGGCTAAAAGGTTCTACGGCTTACAGATACTTGAAAAATATATGTTTGGTTTTAGGTTTGAGAACTGATGAGTTATTTATTATTCAAAGAAGCGTCGATAACTTGCAGCATTTAAGCTTTAAGACTCAAAGGTATCTTGTAATACGCATATAAAGCAAATGAGGAGTAGTAATGAATAAGCTTCAATTATTATTACTGCGGTTTATTCATTACTGCTTGCTTTAGAAAATCTTATGGACGCACACTCGTGTGTTATCTTTCATATTTAACCTTTTTGGGGTTTAAAGACATTTTAGAATAAGTATGCTGATGATGTCCAAATTAGGTCGATAAATAATTAGGAGTATTAATGAATGTAATGAATTAATACGAAGGAGAAAGAAATGAAATACAAAGAAGGACAATTAATTTTAGTTAACTATAAGTTTGCTCAAAAGTTTTTATCTTCATCAGTTGGAGATGATTTTCTCTATTTGGGATTACAAAATCGTAAAAACTTAGCTAAAAGACTTTCTCAAATTATGAATATACCAAAACTTACTCCTGAGTTTCATTGCATAGCTAAAGGGAGCTAGTTAGTTAAAGAGGAGGGAGAATGGATGTTTATATGAAGTTCAACTATGTATCTAAAATGACTAACTATAAAACTGGTTTAGGCTATTGTCATGATTTAAGTAGTTGAGTATCAAGTGGGACAGTTATTGATTATTTTGCAAGAGGAGATAAGTGTGTAGCATTTAGTCCTTTTGATGATAGCGAAAAAGATAGAATGCTTTTAGAGGAAGTAAGTAGAGAAGAAAAGATTAACTGATATAGAAATCATCTTAAGTCTAAAATGGGTAGCCAATATTCTGGTGTTTATGATATGCTAAACGATTCTAGTGCTGATGTAAATATCGATAACGTTAAGGAAGAATTAAAGAGTATTGATGAACAAACATTTTATGAAGGCATTTTAAACTTAGGTAATTTAGGTTTAGAACATAATGTTTTAACTGCTAATGATTGAGCTGAGTTAGTTAAAGAGGAGTTTAGAAACTTCTATAGCATTAGCGGTTTTAATTTAGATAACCTTAGTTCTTATTATTCAATACATGGTAATACAGATAATCCACACATCCATTTTATGTTTTTTGAAAAGACTAAAAAAAGAGCAAAAAGAAAATTAAACATCGACGATATAAAAATGCTAAGAATGAAGATTACAAACAAGCTTATTAAAAACTTTGATTATCAAAATCTTAAGGATTTAACAAGTAAGTTATGAGATGCCAGAAAAGATGTTTTATCTTCGACAGAACGAACGCTTGAGAATGTTCAAAGCCAAGATTACAAAGACTTTATTGAGGCTTGCTGTTATGTAAGAGCTGAAATGGAGAATATGTTCAATAAGTCTTATAAACTTGCTTCACCAGAAGTTAAAGAAGCGATAAACAAGATAGAAACATTTTTATATAAATATGATGATGATTTTAAAGGAAAGATGGATAACTATCAAAAGGCATTTGATTTAGTTATGCAACTAAAGGATGAAACGAATTCAACGTTTTTAAAAAAGAGAATCAATAATGTTTTGAGAACTGAAAAAGATGAGTTTTCTAGACAGCTAGGGAACAAGATAATAAAAGCAATTCTAAAATCAAGAAATGCTAAGAGTGCTAAAAGCTTTACAAAATTATTCTTTTATAACTTGAATGTAAATGATGATTCTTGAAGATTGATTAATAGAGCTAAGAAGATTTTTGAAGCTCAAAGCATTCAAGAATACGAAAACATTATGACTTATTACAAAATATAGGATTTTATTATGGGAAACAATATATCTAAGATAGTTAAAAAAATTATAGAAACGGAAGATTGGAATGATGTTATAACGGGTATCAATTTACTTCATTATAAAAAGATTTTGAAAAACAATATGCCATTAAGAAACGTAGCACTTGTATTGTTTTCAATATCAATTTTAGTTTTCTTCATATTTGCTAGCCTAGTGAATGTATTAAATATAAGTTTTGCTTGAAATATTTCTATTACGGTGTCGTCTTCATTATTAATGGTTGTATTCCTTTGCATGATGTTATTAATAAAGCCTATAAGAATGAAATATAAATGCGACCAGAAGTTTATTGAACATTTAGGCTTAAGCGTAGAAGGCTATTGTCAAATAATTGAAGAATGCCTTTGCATAGCTAAAGCAATTCCAATGCAAAAGTTAGCTAAAAGAATTATTGTGCAAAAGCTTGATATTTTTATGGCTGAAGGCGGAGGTCAATAATGGGAGATAAGAAAAAGAAAATATTAAAAATATCTTTAAGTGCTGTTGCAATACTTCTTGCAGCAATTATCATCTTCCTAGCTCTAGCCATTATATTTAATGTTAAGCGTTTGAAAAGTTTTAAGATGGTTTCAATTAAAGACTTTTTTGCACAGACAAAAGCAAGCATTTATGCATTTCCAAAACAGTTTTTAATTTCATACGCATTTTTATTAATTGCATCGATAGCGGGTATAAGTTATTTATTCTATAAAAATAAACTTAAAACATCATTATTAAAACCTAATAAGTATTGATATAGAAATGAAATTGATAATAGCGGTGTAAGTAATGAAACTTTCGATAAGAAGTTTAAAGTTGCACCAAATTATGTGGGAAATCAAAACAACGCTAATTGGGTAATTAAGTTCGATTCAAATCTAAATAAATGATGAGTGAATGAACCTGAAAGTGATATTAACTCAGTTGTTTTGGGGGGTGTAGGTTCAGGTAAAACACAACGTGTACTATTGCCTAATATTATTTATAATTCACATCTAAGATATAAATATAGAGCTAATTTCTGTATTCCGGATCCAAAAAAAGAAATTATCAAAATGGTTGGTAAAAAGCTTGAAAAGCAAGGATACAAAATATATGCGGTAGATTTTAGCGATACAAAGAAAAGTGTTGGTTGGAATCCATTATCATTTGCTTATAAACTAGCTCATAGAGTGCCGGAAGGGCATCCTAAATGAGAGGACAACATGAATGAAGCTATAAGCGAAATTAATAATGTTGTTGAACAGCTAAAATGAGGGAATAATAGCGATGGTAATCCTATGTGGGTTGATAATGCTAAATTGATTGTTAATGTTGTGGCTCAATTTCTATTGTTGCTTTCAATTGATAGGCCGGATTTATTACCAGAAAGGCATTTCAATTTGCAATCGGTAGCTTCAATGTGTAATTTGGACACTTGAAATCCTAAACAAAAATGAATCTCAATTTGCTATATAAATGCAGAAACAAATAAAAGGTATTATTGAGCTGAATTATTTAAAAATGTTAAGACAATTGCAAATATTGTTCCTGAAACATTAACGGGTAATTTATCACATACTACGAGTGCTTTAAAAAACTTTACTGAAGATGAGTTTATAAAGAGATTAACAAGAGAAACAAAGAGTTTTGATTTACATGGTATTGTATCTTCGAATGAACCTTATGTCATCTTCATTCACTATCCAGACCATAAGCCAAGTAATCACTTCTTAGTAAGTATGCTTATTGACCAAATATATCAAGCTTTAATTGATTGCGCTAATAGTAAGGAAGACTTGAAACTTGAACGGAAGTTCTTCTTCATGTTAGATGAAGCGGGGAACCTGCCTCCAATATTGAATCTAGATAATAAGGTCACAATATCGCGTAGTAGAAATGTATTTTTCCAACTTGTATTGCAAGATTACAATCAGCTTAAAAAATACAATACAAGCACTAATCATAATGTGGATAGCATTATTCGTAGTAATTTGCAATTTACTTATTTCTTGAACTCTATTGATGAAAAAACTTTAAGCGATTTATCTAATTCTTTAGGCAAAAAAGAAGTTATTAAAAAATCAACTTCAAAATCATCTTCAACATCAGCTAATGGTGGTTCATCTTCAGAAAGTGAATCGGTGGAAGAAAAGCCTTTAATGAGTGTGGCGGAAATTAAAGCGAAGAATAAAGAATATGCCATTATTCAAAAAATTGGTTTTCTTCCTATGATAGTAAAAACAAAATTAGCTTACAAATATTTTAGTAATGATAATTATGTTTTTGAATGTGACGAAACAGAAAACACTGTGAATGAATCTTGAAATTATCAAGACTTGCCAGATGTTGAATTACCGGTTAGAAATCCTATTAAAAACTATGACCAATTGAATGATAGTTCAAGAGCCAAGAAAGCTAGAGAGGAAAGCCTCAATAAAGAACTTAATGATAAGAAAGTTCAAAAGAAACTTATTTTGGAAATGCAAAATGCAAGAGGAAGTATTAAGGAGCTTAGCGAGTATAAAGACAAGATAAGCGAATGGCCTAAATTAGCACTTGCTTGACAAAAGATATTAAGTTTCCAACAAAAATACAAAAACAAATTAAGTAGTGAACTAGTGAACGATTTCACGAAGTCGATAAATATATGTTTAGACCAGATGAATGATATTAAAACAGCTGGAGCTAAAACATAGAAGAAGGCGAATGAAAAGATATTTTTACAGAATTAGAGTCAAAAAGAATCTTGAATCTAAGAAAACAAAATCATATGCAGTTTGCTCTAACTTTAATGAGGCAGACAAGATAGCCAATGAAATTATAGAAAAAGGAGATTATGGTTGGGTGCAAGTTGAAAGAGTAGAAAAGATTAAACATTATTTTAAGGAGGTTTAATATGCAAGTATTTAATGCATTAGAAAACGAAGAAATTATTAATCGTAGAAACTATGAAAAGAACGGAACTAATTTTGAATTAATTAGTACAAAAAGTGGTTTGACTTTAGCACATAATGTTTTAAATGATAATAGTCAAGAATACTATGAAATGAACGAAGGAGCTTTTAGTGGACGCTATTACAAAATTGCTGAAAATAATCAGCTCGTTGGCGTTGCGAATTACCAAAATGATGAATTAAATGGTGAAGCAACAATTTATAACACTAAGGGCGATATTCTTTATAAAGCACATTTTGAAGATGGTTTATTAAATGGGGCTGTTGAAGAATATAAAGACGGCGCGCTAGTTAAAAGCACAATGTATGAAAATGGATTTAAAACTGGCGAAGAAGTTGTATATGATGCTAAAGGAGAAGTAGAAGGAAAAACTAATTTTATTACTAATAATGAAGTAAAAGAAAAAGAGGTAAAAGAATATAAAGAAGCTGATAAAGAAAAGAAAGCGGAAATAAGAAACAAGAACTTTAAGGAAGTTTTGAAGAAATTACCTGCTCTTGCATTAAAAGGGTTCTTATGTCTTCAAGCATATTTAGTGGGCGGTTTGCCTTTAGTAATAGGCATAACTGCTATTAAATATTTCTTTGATAAAAGAAAAGAAAAAGCTTTAGCTAAAAATGAGATAAAGCCGGAAATTGCAAAGAACTTTAAGTTGAGCGATGGTGTTGCTAAAGAGATAACAAAAACGTTTAATGAAGCAATGAAAGAAATTGTTGTTTCAACTAAAGAGGCTGTGATGAGCCAATACCAAAAGGATTTAGCAAGTGGAAAGATTATAGCTGACGCATATGATAATGCGAATATTGAAAAAGCACAAAATGCAGCTATGAATAAAGAAATTGCTAAAGAAACAAAAATTGAAAATCCAAAGTCAAAGGCTAAAAAATAGGAGGAACTATGGAAAAGAAGAAAGGAAAAGGAAAAATTATGCATGAAATTAAAAACAATAAATTTGATGAAATTTTATTAAGTGAAGAATCAAAACAAAAAATAGATCAAGATTTAAAACAAAATGGTATCAAAAGTACCTTAGCTAAAAAATATCTTGAAGAATTCAAAAAAATGGAACCAGAAAAATATGAAAAATTTAGAAAAGAAAGCAATAAAAAAATATGCAATAAGCTTTTTGCTAAATAGAAAGGAAAAAGAATGATTGAAAAAGTTTTTTGTTTTAAGGATTTGTATTATCTAACTCAATATGCATTAAGTGATATTTCAGATGAAGAAGTTGTAGCTAAATTTAACTATTTAGCAAAAAATATCAAGAATAATACTATGCGGGATAAATATAAAAAACAAGTATTATCTATAGTAGACAAAATGTCTAATGTAGACGATGATATAAATCAAAAGATTTTTGATAAATTGTTTTATGTTGAAGATACAAATGAAAAAAATTTGGAGCAATACATCACTTTATTAAATATATGCAATGTCGAAGATTTAGAAAATATAGAAAATAATAGTTTAGATTTAAAATTCTCATTTAATGATTTTTTTGGTGAAAATGGTTATATAGAAAGTGAATATTTATTTGATTTAGTAGATGAAGATTTAAAACAAAATGGAATAAACAGTAAATATATTTATTCACTTGGTGATTTAAGTAGAATCGATACAGAATATGTTTATATTGATGATTATTTAAATTTTCATCCAGAAGATATTGATGACATAAAAAAAGAATATATAGAAAATTTAGCTTCTTTAAAATTAGATGAAATCATTGATTTAAATAATAAAGAAAGTATTGATGCAAATAATAAGGTTGAAATAACTGATAAAACTGTTAAAGAAGCTCAAAAACATGTTGCTGGTATTGTAGAAAAAGAAAGTGAAACAGCAAAAGTTAAAAATACAAAATAAAGGAGGCAGATGAATAAAGTTATATTAACGGGTAGAGTAGTAAGTGAAGACTACTTTAAAGCTAAAACAACAAGTGGTAAAGCTGTTTTAGGGTTTGTGATTGCTGTAAATAATGAATATGGTGGGGAACCTGATTTTCTAAATATTACAGCTTTTGGTTTGCAAGCAAGCAATTTTGAAAAGTACAATAAAAAAGGCGACTTAGTCGAAGTTATTGGAAAGATAAAGAGAAATGCTTATGTGGATAGAGATAATCATAAGCAATACAAAATGGAAATTGTTGCTGACAATATTATTTATAGTGCTAAAAAAGTTGATAGAAACGCTGAAGGTAATGATGAAGATATTATTTATAAAGATGAAACAAAATTACCTAAGGAGGAAAAAGAAAAGGTTGTTAAAGCGAGACTAAAGAAAAAGGATGAAATGATGGAACCTTTTAAACAATATATTGGTATGGATCCATTGTGTCAAAGCGTCTATGATAAATACGAAGAAGAAGCTTTTAACAAAGATGTTGAAGTAAAAGATGAAACTAAAAAACACGGTCAAACATTGTAATGAAAAACTATTTGAAATTTAAGTTTTTTATTATTTACATAGTATTCTGGATATTAATGCTGATGATTTTAAGTTTATCTTTTGTGGCCGTTAATGGAATGTATGTTGATAAAGAATTGACAGAAACTAAGTATGGAGTTATCGAAGGAATAATTTTATTTCTTGGAATGGCGACTTTCATATATCCAGTATATTGAGTGAAAAAACGAATAGAATACTGAAAACAAAGTAAGAAGAATAGTGATTTGCATTAAAATTATTATGTAAAGGTATTATTATGAAAAAAAGTAAAAAGTTTATGAAGTTTATGGCAATTGGAACGATAGTTCCTTTAGCAAGTTTAACGACGAGTTGTTTTGGTCCTATTTTTGAGCCACATCAACCTCCAAATCCAAATCCGAACCCTAATCCAAACCCTAGTGGCGGAGAAAGCACGGATTGGAGCGATGTGGATGGCTATGTACATTATGATGCAGAAAAGAATCCAAAAGATTTTCTAAAGAAGGACAATCAATATTATTTTGCGCAAGTATTGCATAATGAGCAAAAATATGATGCCGATGTGTATAGAAATGATAATTGAAAAAAGAACGAGTATATGCCTAAAATCAATGTTCGTTTAGACTTGGATAAAGACTGGTATACTAGCCAACCTGAATTAAACGAAATTAAAGATTTATTCGATGTTGATTATAGCGCGCCGGTGAAGTTGAATAATATGATGAGAAACTTTTTGAATCCTGAATATAAAGATTTAGGTCTTGTGGATTCAAAGAATTATATTAATTCAATGGCTAGTTATTTAGAAGGATCGCAAAAAGATGTATTTGCCTATAAGGATAGTTATCATGGTAATAAATGAGTGTTTGAGGACCAAAAGGAAGTAAGAAAACACTATCACTATTATTTTGATATGAAGCTTGATAATTACTATGAACAATTCATCAATGATTTTGCTCTTAAAGGCACAAGCAAGACATTTAAAGATATTGCTATTGATGGTTTATATAAACAAGGAATGTGGAATAATTATTTCGACTTAGGAAAAGATTATTCAAAATGGTTTTATTTAGCTTTTACAAGAAGTAATGTTGAAAAATACCATGAAGGCTTTAAGATTTATGAGACTGCAAAACAAAACAAAGATTTCATAATGCAATTGCCTACGGGTGATGATTATAATCTTTGAAATGATTTAAGTAGTTTTGCTTGGATGGCTGGTAAGATGGTTAAGCTAACGTTAGTTAATAACGAAGCTTATAAAAAGTATTTAGAGTTATTGCAAATGTTTGGAACTATTCAAGCTGGAAATAAGAAGTGGTTTGAAATTGAACAAGTATATAGAAATTATTTTAAAGAGTACTTGGACAAAGCAACAAATGCATTGCTTGATGTTCTTAAATACGGAGCAGTCTTTGGCGTGGAAATGGAAGAATTAGGCAAAGAGAAAAAAACCTTAGTTAAGATGTTTCCAGTAGCTATTGGGGATAATCAATTTGCTGATTATGAATATAGCTACGTATGGGCGTATAGAACACTATATGATGTGTTAATGCCTATGTATGGGGCTAATGATTGAGAAGTGCCATATAGTTTTACTGAATATGATAATCAAAAGTCAGGCAATACACCATATGCTCCAATTTACGAATACATAAACTTTTTTACAAATACTGTTTTTAAAGCTAAAGATAAAAGTTTTAAAGAACTTGAATTAAACACTAAATTTATGAATAAAATGCAAGAGGCTGAATATAAAAAAGAAGCTAAAAGTAAGTTTCATAGCTGAATTGCAATTTGGAATAATAGAGTTAATGGAAGCTTGGATACAAGCACTTTAGATTAAAAATTATGCTAACTTTACTACATAATAATGTGTGCAAAGAACTATTTTTCTAATAGATGTTTTTCTATTAGTTTTTTTGTATTAATTTATTAAGACAAAAAGGAGAAATTAAAAATGAAACCTATTGATACAGAAGGATTAAGTAATTTAACAGAGCAAGTACAGAAAGTTGGACATATCATATTTGGACTTGTGGCTGTGGTGGCTGTTATTAGCGTTATTATTACCTCGATTGTGGCGGGGATACAAAACTCACATGAAGATGATCCACAAAAAAGAAAGCAAAACAATAAAAAGATTTTGTGAGCTGGAATTGTATTAATTCTAGTTATAGTGGGTTGAGGAATATTTGAACTAGTTATTAGTATTCAAGCAAGCGGGGGAAAGACTAGCTAATGAGTGGATTTTTTACCCGTGTGATGGAGATTTTCAAATATGGTCCAACATATGTATTGTATTTATTGCTATTTTTTGTATCGCTAATTGTTTTTATATTATTAGCGATTCTATTATCGGTTGTTAAACTAGCAACTTTCGATTTATTAAACTTTGTTATTTTTGGCATAATGCCGAGACAAAACATTTTGGACCATCAAAGCCCATATATGTATGTTAGATTTGCGATAGTTTCGGCGGTTATATGACTGGTGATGTTTTTTGTGCTGATTATAAAGTTCAGTTTTAGTGAAAGCGAAAAGAGTGTATCACTAATGAGAAGTGCGTTGGTATTTAGTGTTAAAAGTTTGTTTATACTTTTAGCATTTCAAATTACTATTTTACTTTTTAATCTATTTGTACAAAAAATAATTACATTGATGATTGGCGAAGGGAATGATATTGTTGTTGCTTTTATAAATGGCTTGCATAGGTTGTTTTATCCTCCAAAATTAGCTGGGGATGTTAAAGATATTTTAATTCCTGCGACTACAAATCCTTTTGGCTTTACTATCAACTTGGATATTAGTTTTGGTAGTTTTGTGGCCTTAGCAAAGAAAACTGAACATTTAAACATTGGTAATTGAGCATCCATTTTAATAACTGCAGCGTTTCTATCAATTATTGGAGCGGTGTTTATTGCTATTCCACTTGTTTTAGGGGGGATGGATGCCATAGGAAAGATATTTCCGTTATTCTTCTTGTATTTAATATTTCCAATTATTTTACCTTTGAGCTTGCTTGATGATGGCAAGAAAGTAAGGATATGAAAGGATAAGTATGTGGGAAATATGTTATCGGTGGGGGTATTTTTCCTAGGACTACAAATACTTTTTGCTTTCTTCGGTGCTGTAAATCTATTTTTAACGCAAATATTCATTGCATCATCAACAAATGGTTTTGGATGAGTATTACAAGCTTTAGCTTCAATAATTATTTATGGTGCTACAGCGTTAAAATACAGCGAAATAAGCGAAATTATAACTTCATTTATTGGAACTAGTGTAAGTAGTAAAAACACTATTAAATCGGCTTCAGCTGCGGGAAATAGTGCTGCAAAAACGGGTAGAGCTGCTCAAGGTGGAGCACAGCTTGCTGAAAATGTAAAGAAGATGGGCTGAAAAGGATTATTCCTTAAACCTAAAGAATAATGAGAAGGGAGAAATGTGCTACAACCTAAACGATTAAAATATAAGAAGGCAAAAGCCACAAGAAGATTTAGTTGAAAAGATATTATCGAGTTTTTTGTAATAACTTGTATATCTTGATTTATTTCATTTGTTGCTTTGTATCCAGTTAAGCAGACTTATAAATGAATTGTTTTTAGTGTACTTTTGGTTTTATTGTTTATTTTGATATTACCAACACATAAGAATAATTGCAAGATATATGAATTATTAATTAGAATGTTTAAATTTAAGGCGATGCCAAAGAAGTATTCTAATGTGGTGGATGACAAGAATGTAAGAGATAGTTCTGATTTAAATCCATATTCAACATTAGTAGAAAACGATGTTGTTAAAAACAAGTCATTTTCAAATAAGTTGTTTCAAAAGAGTGAACATAGTAATTACTTTAGTGTTTTTAAATTAGGTGGAAAAAATATATGAGCTGAAAATGAAAATACTAAAGTGGTTTTGATTGAAAACTTTGCAAGAATATTGGATAGTTTTAAATATAAATTTAGCATAGTGAAATTAGATGAACAAATTGATTACAGACGTAATTTAAATTATATAAACACGATTCAAAGTAAAAAGATGTTAAATAGCGGTGGAGAAACAAAAGAGTTTTGAAATAGTTATTTTAAAGCTAATGAACTTGATTTTAAGAATGAAGTTAAAAGCAATTTGATTGAAACATTTTATTTAATTTTAAATGCTCCAACATTAGAAGAATTGGATGAGTGTTATTCGACATTTATTCAAAAGGCTGAAGATTATGAAGTTATTAGCTATGAAAAGCTAAGTGGCTTTGAGTTATTATCATTTTTGAATAAACTTAATCACTTTAATAAAAGCGAAGAAGATATTGAAACGTTTTTAAATGAAAGTGATATTTACGAAAGTGAATATAGTTTGGACCGCTTGTTCAAGTATGAAAAGGTGGAGTTTAAAAGCAATTCATTAAAAATTGATGATAAGTTGTATAGCGTTAAATGTGCTGGGAACATTCCAATACGCTTGAATAACGAGTGATTAAAGAAGTTATTCGATGTATCGGGAACAGTTGTGTGAAACAATTTTCCATATAACGATCCAAATGTTGTAAATAAGATAATCGATAAAGCGAATAAGAAAAATATGGACGCTGGTAATTTGGATAATTCAATTATTAATAGATTCGGTTCAGCATTAGAGGAAGAATCGATTGCGGTTATGATTGACCAAATACAAGCGGATAATTTTAAGTTATTTGATTCAAATATTTTTGTTTTAGCTGAAGCGGAAAATGCTGAAGAATTGAAGCAAAATAATTTGAAAATTAAGAATGGTTTTACAAGATCTAATTTTGGAATTAATGATTTGACCTTTAGACAATTTGAAGGATTTTTAGATATGTCTAATTATCCATTAAGCAAATTAGATAGAGAGTACTATCAAATCACAAGTATAAATAACGCAATAGGCTATCCATTGAAAAATCTGCCACTTAATGACGGAAACAACTTGTTGCTTGGAAATGAAGTGGGAGGAAATGATAGTCCTATTGTATGAAATATGTTTAGCTTGACTTCAAGTAGAGCAAATCATAATGTGATGATATTGGGAACTTCAGGAATGGGCAAAAGTACTTTAACCAAGAAGATTCTTACTTCATCACTGGCTGCGGGGAATATAGCAATTATTATTGATCCGCAAGCTGAATATGTTAAATGGGCAAATAAAATGGGAGGCCAAATAGTTGATTTAGGTTCTGGAGATGGAACGGTTATTAATCCATTACAAGTCAAATCATTTATTGCGCGTGAAGAAGATGATGCCAATGTTAATTTCAATACTTTAATTAATAATCATTTGCTTTGGTTAGAAAAGTTCTTTAGTTTCATTTTTGATGATATGAATGAAATGAAGAAGAACATTTTGCAACTTGAAATTATTAAGTTATATCAAAGTTGGGGCATTTATAGATTAAAGGAAGTTAAAGAACTCAAAAACGATGGATGACCAACTATGGATGATTTGATTAAGCAAATGAAAAAGTTTGTAGCTCCTAAAGACCATCACGATAAAGAAGGCTATACTAAAAAGGTTTTAGATATGGCTGAATTATTAGAGGGTAAATTTGGAAGATATGGAACTTTGGGTGCGTTATATAATGGTCACACCAATATCAATATTGAAAACGATTTGGTTGTTTTTAAAACAAGTAATTTAATCGATACTGAAGGTAGCGTAAATGCAAGAATTGGCATTATGGTTTTAATTAGCTTGGTGAATGGCTTTATCTTTGATAATGCTTTTAATAACAAGAAAAGAATTAACGAATATAAAGAAAAAAATAAGATTCGTTTAATTAGCAAGAAAGAAATACAAAAGTTAACACGTTATTGTTCATTTTGTATTGATGAAGAACACTTGTATATTAGTGAAAAGAATATAACTACTTTGAACTATATATCAGATACTACAAAGCTTGTGCGTAAGCTTGATTGCGGGACAATACATACCACGCAAAATCCTAGTGATTATGCTTCAAGCACGGCAGTTGAACAAATTGCTAAAAAGATTACGGGGAACTGTCAATATTCATTTTTTCTAGGGTTAGTAGGAGATGATATTGAGGCGGTTAAAAAGTTATACAAAACGGCTGAAGCTCCATTACTAGATTCTGAAGTTAAGTTTATTTCAGGAAGAAGAGTGGGGAAAGTTTTAGCAAGTATGAGTAATGCACTTAGATACAAAATTAATCTACATTACAATCCAAAGGAAAAGGAATTATTCTTTGAAAAGGGGGAAGATGAAAACATGAGAAATTAGTCAAGAAAATAAAAAAGGTGAATTATATGTAAAAAGCTCTAGAGCCTCAAAAAACGGCTCTAAATGTATTTCTACGCGTCTTAGTGAACCAGATGATGTGGCGTTATACGAAAGATGGGTAAAAAGAATTAAACAAAGTGGCTCTACTCCGTATGTAGAGCTTAAAGATATAATTCGCAACGCTTTAATTAAACAAGAGAAAGAGGAAATAGCTAAAGATTTCAAAAATGATTTATTTTATGCATTAAGAAAAGTATCTTGAGCGTCTTTGACTCCATTTTATCGCAAGATTATAAATGAAATTAACAAGCAAAATATTGATTTGGTTTTGCTTGATTTAAAAGTCAATATGTTGTTAAATGCTCTTATAAGCGATAAAGGAATGTTAGATGAACCTAACAATAATTTGCTTAAAGAGCCAGAATATGCAAAGCGTTTGCGTGAAAGAATGAATGAATCGGCTTCTGCATGGTATAAGAAGGTAATTAAAAAGGTAAGAAATGAAGACAATTCATTAAGCAAGTTTGCAGAACAATTCGAAGACAATGAAATTGAAAAAGTGTTTGAAAAAGAAAATGCAAAACTTGAAGCTTCAGAATTAAATAATGCTAAAGAGGATGAAGATTTTGGTGATTTTGATTTAAGTGAGTTTTTTAAAAAATAAAATAGTCTTTAAACATGCACACGTGCGCACACGGGCATAACTAATATGCACGCGGAGGCACGGGCGAAGAATGAGGAAAAAATGAATAAGAAAAAGATGTTAAAATTAATTCCATTGAGTGTATTGAGTATTGCGATTCCGTCAGTACTTAATTTATCGTTAAATACTAATAAAAAACTTCTAGGTTATGGAAAAGATTTTAAAGCTCCTGAAGAAAAACATAAGATTGATACTAATAGTTTATTTTCCAATTTTACTAGTATGGAAGATGATGAAATTGCTAAAAATGAAAGACTACAAAATAGGTATCAAGTAAAATGGTCACAACTATGTGATAAAGGATTATTGTATGCACAGGACCAATGGGTTGGTTATCATAAAACTGGCCCAGTAAAAAATAAAATTACGTCAATAGTTTCTAGGCCAACTTTAGGCTCTTTTGAAGTGCGTTATGAAAAAGCCTATTACACAAATCCTAGTAATCATAATAGTTATGGCTGGTTTTGATACGATGATAAATATTATCTAAATTATTTGCATCCTCGTGAAAAAGAGAATTGAGTAATTTTTGCTTCTAATTTTATAGAACAATTAATAAAATGAGACAAAAACAACTTTATGGAAGAACATAAAGGTGCTAGTTTTACTAACCTGTTTGCTGAATATAAAGTTTCTCAAAAAACATTAGAGTTTTTAAATAATCTTGAAACTGATAAAGATAAAAGGGTGTATAGTGGGCTAACTGATGATAAAAATAAAATGTTTTTGTGGGGGCTTCATGATTTTGATTGTGAAATTGAAGGAATGTATGATGTAAGGTACTATAATAATAAAGTATTAAATAATCTAGAATATGCTAAAGTTGGCTGATTCATAGAGGCTTTAAAAGAGTGTGTTTCAAGTGATAATTGTTTGAATAGATTTGAATATGGGGTAAATAAGAAAATTGACCAAAATCACTACTCTATAGATTTATTAAGCGATACCTTGGGCGATTTGACTACATCAGTTGATGGAGTTTTTAAAGACAATGGAGGCAAATCGAATATAGAACATTTAAACAAAATATTCGATAAAGAATATGAGTTTGAGTATGCTAGTGGAATTAAAGTTAAATACAAATATGATTACAAGATAAAAAACGAAGCTGTTGGTGAAAAGAACAAAATTGATGTTTATATTAAGGTTTTATCAGTCAAGATTGATGATAAGGAAATTATCGATAAAGACTTGGATAAGATTCTTAATAATATTGAAGACTGAACAAAAGAAAACATTAAAGCTGAAGCTGTTGGGAAGTGGAATCAATTTTTAAGGGTTTTAAAAGATATCGTTTTAGGTAATTTTAAAGTTTTTGATTCATTAAAGGTAAATATTTTAGAATCAGATTTGTATAAAAGACTTAGTGGAGCTTCGTTAGCTTCACGGTTAACTATATCAACTTCTATTACTAATCCTGATGGAAGTATAAGTCAAATAGAAGCAAAGACAAAAGCTAATGGTGGCGATTTAAAAAATCTAATTAAAAGAGACGAACCTGAAAGGGTAAGCAATAAAGAACTTGAAAAAGATTTTGTCGGTAAGCCATGATTTGGTGGAAGGTATGTAGTTAGAGGGCCAATTAATATTTCTTTTAAAGCTTCAGAGGATGAAACTGAAGTTTTAATGGTAAATAAGCAAAAGGTTAGTGTTTTAAATAGAATATTTGAAAAGAAATTAATTGATGAAAGAGTTAATGTTAATGATGCATCTAAAATACCTTTTGATAAAAACTCGGGAAGCGGTAATGGCGAAGGCGATGTTGCAAAAAATGAATATCTTGTTGAAGTTTATAGATACAAGAAAGGCTCAAATAATAGTGGAGAGCCGGAAGAAAAGTATGTAGTTATTTATGAAGTAATTGGAAATCAGTTTAAACAAAACATAAAATATTATGCTTGAGAGCCGGAAAAGAATATGGATCAAAAACAAATTATTTCTCCATATATGCTTGATTCAAAGGGAAAAGAGTTAAAGGATAAAGAAGGCAAATTAATAGTAAATAAGAATTATGATCCAAGTATTGATAAAGAAACAGGAACTAAAAAGCAGATTGTTTGAATTAAAAATAAGTTGTTTAGTGATGAATTAAAAGAACAACTTCGTTTTGTATATCCTAATATTAAAATAACTACAGATAAAGCAGATCTTGGAATCTATGCTGAAGCTAATGTGCTTGGTAAGGGGGCATTAAGAAACTTGGTGGTTGATAAAGTTTTAGAGTCATCTTCAAGATACTATAAGTGTTTGTTGTTTAGAAAAGAAAATGACAAATACGTTGAGATTGGTAGGAAAGAACAAAAACTAATTGAAATACCGGTAAATAATGGGACAAGTGAATATAGTTATATGTCTGAGGAAGGCATTTGGCTATTATTTACTGTAAATGAAAAATCAATATCAAATGTTAATTTGGTATTAATTGATGCAAAGAGCGAGCCAAAAAGTTTTTTCTTGGATTCAATTGAAAACAAGATAGAAAAAATTAATAATCAAAGTGAAGATTATTTTGAAGCATTTTTAGATGGTGGCAATTGAATAACTACACTATTTCAAAATTACTTAAATGAAGCTTGTGGTATCCAAAATGATGACTTGGGTAAATTAGGATATGACAAGATGATTTCACTATATAAAAACTTTGTAGACCAAGCGTCAAAGTTTAATGTAGAAACAGGAAACGAGTATGAAAATATTTTTAAAGATAAAATAACTTGAGGTAAATTAGAAAATCTTAATGGCTTGAATTATGGAGATGAAACTAATGAAGCTATTAAAGAAAATTATAGAAGACAAGTTAAAGAAATCGTGCTTAATTTTGTTAAAAGAAATATTGCTAATAGTGCTAAATTAAAGGCAATTGGAATAACTGAAAATGAAATTAATGGTGGCTGGGTAATAGCTAATTGAAATACGCCAGAAAGCGAAAAGCGTTGATTGGATAGGGCTTTAAAAGTTTATATTAAAGATATTAATGTACCAGATAATGACGGTAAGGGATGAGCTTTTACTTTGCGTGGAACGGGTAAATACATAAATCACAGCATAACATTTAATTTGAAAAATAGTGCGTACCACGTGTACTATCCTCCTATTGATTTATCATCACTAGACATTCAAAAACACTTCGAAATTGAAATGGGAAAGAATATTAGTAATGATTCAAAAGAATTAAAAGAAAAGATTAGAAATAAGTTGATAGGGCTAATAGAAGAACAATTCAACTTATACAAAAACAAAACTGGCATAGAAATGTTATTGAATAAAGATATTAAAGTAGAAGGACTAGATAACGTTTTAGATTTACTAGTACAGGGGAATAAGTTTAAAGATGGTATTGATATTAAGATAAAAGGTATTAATGCTAATTTAATTTCAAGCACTAAGATTCATATTATTAATGATACTGAAGCACCAGCATTTGATTTATCACTACTTAATGATACACTAGTAAAAAATCCTATTGTTATATCGCAAAAAGAACCTGAGAAAATTACCAAAGATATTATAAATAAGTTAAATGAGATAACGATTGCTTCAGGTATCGAGTTTAAAAAAGATTATTTAGTTTCACTTGTAAATGCTTCATTCTATTTACTTGGTTTAGTAGCTGATATTGATAAATTAAATGAAGCTAAATATGAAAAATTCAAAAAAGTTTATGAAGAAACTAAAGCAAAGGTAATGGCTGAATATAATACTTGAGAAATTGAAGGACATAAAGTTCCTATTCCGTATCAAATAAATGATACTGAGCTATTTAAGAAGTACCACGAAGCTTTAGAAAAGGCAATTAAAGAAATACAAAACGATAACTTTAATAAAAGCTTAGATTATAAAGCTAGAGGCAAGTATTTTGTTTTATTACCAGTTAAAGAAGGCAATAATATTAATTTTGCCTATGCTATAGTGGTGAATAATATTGAGGCAAATTATAATGCTTTTGATGATAAATTATGATTTAAAGAAATACAAAACGATAAGCATAAAAAGCCAAATGGTGAAGATTTAGATGTTGATAAAACAAAAGCAAGCCATAAAAGTAATTATTGAATTTGAATTGTATCGGTTATGCTTGCCTTAGGTTTTGCTGTTGGTGGCATATATGGTATTAGATGATACTTGAATAAAAAAGGCTTTAAAGCTGGCAAGAGCAAGAAACAAGTTTCAATTAAGAAATTCAAAGAAATACAAATCTATAAAAAAGACAGAATTAAGGCTAAGCCGGTTGTTCTTAGCGATGAAGAAATTAATAAATTATTTGCAAAATAAAAAAATGTTAGGCACTATATTTTTAACTAAAACATTATTAAGAAAATATGGGTGAAGTTAAGAATTTTATAAAATATTGTTTTTTTACTATATAATGTAGACATATGAACCTAGAGAGATACACGTAAGTAATCGAACAGTAGCAAGCTTAGCGTGTAGCTACAATTACTCTAGGCTGGGTCGTCTTAAAAAGAGGTTTTATACCTCTTTTTTTGTATAATTTTATTATGTCTTTAGATTTATGAAAACCTTTAAAAATTAATAACCCATTATTAGAAAATATTTTCAAACTACCTATTGATGGCAAAGTTTTAAATAACGGAAAAATTCAAGCCCACCCTTTTATAATTTTTTAGTAAAAGATTATCAATTTGTTTAAGAGTAAACAATGCAAATAAGATTTGAAAAATTATTAATTTTTTTATTTATTTGTAGTATAATTTAAACAATAGTCTAAGAAGGATTTTGTTATATGAGTAATCGAACAAGCAAGCAGCTCATATTTTATCTTTAAGCGCTTGATGATACGCCTTAGACTCTAGTTCGTCTTAAAAAGAGGTTTAATCCTCTTTTTTTGTATAATTTTTATATGAGTTTAGAACTATGAAGACCATTAAAAATTACAAATCCTTTATTAGAAGATATTTTTCATCTAAATATCGATACAAAATTTAAAATTCATCCTTTGATAATTTTTGAAAATACAAAAAATAAAACTTATTATTGTATAAGACTTCAAACGGCAAGAAAAAGTTCTAGCAAATATAATTTAATAATTGATAATAGCTCTTATCAAACCAACGAATATTGAAAGAATCATGAAAGTGTAGTAGTTACTAAGGATATATTTATTATTGATAAGGAATTGCTTGAAAGTAATATAGATAAAGCAATTTATCAAGATACTTCTGTATTAAATAAAAATGATAAATCATTGATAGTCGATGACCTAAGTCGCCGTATAAATGCAATTCCTCCCGATTTAAATATAATAAAGATATCAAATAATTTAAAGCACAATTTAGTTTTATATACTAACGATGAGTTAATAAATAATCAGCTTTTATCTACATTGTCTAATAATAGTAATAAAGTTAAGCGTGCAACAAAAAACTATTTTGCTAATAATTTTTCAAAATCCCAATTCCTAATTAACACAAACACTTCTAATACAAAATATACAAAAGAAGCTTTAAAGAATATTAAATTATGTATTGGTAAAGAGAAAAATATAATTAATCAAAATGATTTTGAGTATGAATTAGATAAAGAAACTAGTAATTATTTTATTAGAGAAATTGCTCATAATGATTGAAAAGACTTAGATAAGACGAAGGATAACGTTAATATATATTCTTTTGAAAATATAGGTTTTTTATCTAAAAGTAGCAAAAGTGAATCAAGCTATATTGATCCATACGATAATAAAAATATCGAAAAAGCTAAGGATAAAGTAGCTAAGGAAATACCAATAAAAGAAAGCAAAACAGACAAAGACAAAACGAAGCTGAAGTAAAACATATAAAATTATTAACGGCGTGAAAACGTCGTTTTTAATTGATTATGGAGGGCGCTGTGAAGAACAAAATGAGTAAAACTTTAGCTGACAATTGAAATGCTCAAGCAGAAGTTTTTAAGAATAGCGAAGAGCGTAAAATTAAATATAATGTTGAAAGAAAAATAGAAAGAGATATTTTTATTTGGGGCGAGACAGTAAGAATTATTTACTACATGTATAAAGATAAAACTACAGGCAAAAGACTAGTTATTTATCCGAGTGAAGGATTAGCTGATAACGGTAGAAATAAATATTATAAAGAGTGCGTCGAAAAATGTGTTGAAAACAATATATACGCTTCAAAAGGTGATAATAGTAAGCTACTTATTCCTTATCACGTTTATTATTACCATAGGGAACAATTAAAGAAAGAAGTATTAAATAATTTGACTTTAAAAGACTATAAAAATCTAGAGTACAAGACTATACAAATAGATATTGATGATGCTTATATTAGAGTGTCTTTTAAAGGTAGAAAAATAAAGGTTAGAGTGCGTATGGCGACTATACATACTTTGCTTAGCGGAAGTAGTGTAAAAGCCATAGAAAATAAGACCTCTATTATGCAGTTTTTACCACTTGCAAGTGAGTTTAAAAACTTCAATAGTGAAGAAGATTTTGAAAACAAAATTAGAGAAAGTTTAAACAGTTTATATAAAGAAAATTGTGAAATTATTGTCTCTGGAGATGGTGCAAAATTCATAACTACAATAGCTGATAATTTAAAATCAACGAGAGTTTATGATCCATTTCATTTCAAAGCAAATATGTTTAAAGCTTTTGGATATAGCAAAAAAATTAATGTAAGTAATAAGAGATTTTTTAGGGATTATAAAGATGTTTATCGCGTGTTAGACAACTTGTTTTCTAAAGGATTAATTTATGAATTTGAGAAAGAACTTGACAGTGTTTTAGAGTGAATAACAAAGCAAGAGAAAAATAAAACTGTATATGCTAATGCTAAAAAGTTCAAGAAATATTATAAAGAAAATAGAGAATATATTTGAAACTCAGTGCTAGTTAAAAACTACTTTGGAGGCTGTGCTGAAACGGTAGTTGGGCACGATTTAAAGAGGTTTTGCTCAAAGAAATTTGCTACATTTTCACTAAAAACAATACAGTTAAGCATTGCAAAAAATATGGAAACAAAAGCAAACTTATTTTTCATTTAAGCAGTATTTTTAACAAAGTGCATGCGCAAAAGCAGTATTAATATATTTTTAAAATAAAAATATACAAGTTAAAGTGCATGTAAAAGTGCATGCGAATTTAGGCAAAAACGAAAAAAGTGCATGCGCCTAGAAAATATGAAAATATTAATTTTTGATTTATTCTATGAATAAATCATTTCAAGATAAAACACGCTATGGTAAATAGCTTTGTAGTGTCTTTATCTTGTACTATTCAAGTAGCGCGTGTGCGCGCAAAAGGAAACTGCATGCTCTTCACATGCAGTATTTTCCTTTGCTACAAAATGGATTATGATAAAATAATTACGAAATAAGAAAACGTAGAGAATCTTATAACACACCTTTTTATATTAGTTCCGATTTATTAATTAAACAATTAAAGGAAGAAAATAAAATACTCAAATTGGAGAATGCGATAGCAAAAAAAGTGAGCGCCTTGGTTCAATTGAAAGACTCACTAACAAAGAAAAATTCCAAATAACAATTGAACTAAGGCAAGAATTTAAAAAGCTATTTTTTATTAAATTAATATTAGAAAAAATTAAATTGAAAAAGTCAACTTTTTATGAGATATTAAAATCACAAAATAAACCTGATAAAGATGAAAATTTAAAAAAGGTTATTTTTGACTTATTTAACTATAATAAAGGACTATACGGTTATAGACGTATTACTTTTGCTTTAAGAAATAAAGGAATAATAATCAATCATAAAAAAGTTCAAAAATTAATGAAAGCAATGAATATTTTCGGCAAAACGCTAAGAAGAAAAAATAAATATTCTTCATTCAAAGGTGATGCTCACAAAAACATTCCAAACTTGCTTTTAGATAAAGAAAATATCACAGAAGATTTCTTCAGATACAAAAGAAATTTTTCAAATAATAAATATTTGAAAATACTAGGAACAGATGTTACTGAATTTAAATTAAAAAATGATGAAAAAGCATATTTTTCTCCTGTAGTTGATTTTGAAAACAGAGAGATTTTAGGTTATTCGATTTCTAAATCGCCTAATTTAAGAATGGTTGGTAAAATGTTAGAAAACGTAGAAGAGAATGGCCACAGCTTAAAAAATGTATTATTACATTCTGATCAAGGATGACAATACACTCATCAAGATTATATTGATTATTTGAAAGAAAAACAAACAACTCAAAGCATGTCAAGAAAGGGAAATTGTTTAGACAATAGTCCTACTGAATGTTTATTTAGTGTTATAAAAAGAGAATTTTGATTTGGAGAAGAAAAGAAATTTAATAGTTTTAAAGAATTTAAAACTGCTTTAGGAGAATATATTTCATATTATAATAATGACAGAATTGTTAATAAATTAAAAGGACTTAGTCCTGTCCAATACAGGAATAAGTCCAAACATAATTAAAAAGTCCAATTTTTGGGGTTCATACCAAATGCCGTTTTCTCTTTTAAATTTCTAACTTATCCCACTAATAATTTACATCACCAACAGGTCTTGCCTGTTTTTTAAAATTGGGAACTATAAAAAATTTTGTGTAAAGTCTTAAAATCATTTCCTGATAATATTTTATCAATTAAGAAAGGATTTTTTTATTTATGAAAAGAAGGTTAATAAATCCTGCTATTGATGAAATAACAAAAAAATTTAGAAAATTAAAACCAAGTTACTACTGGTGATCTTAATATGGCTATGAAAGAAGTATTTCAAAATACAATCCAAAAAATGATGAACAAAGAATTTGATAATTTTATAGGTTACGAAAAAATGATAATAAAGTTCAAAAAAAATTATAGAAATGATTTTTTTAAGAAAAATGTTAATAGTCAATATGGCCAAATGGAAATTGACATTCCAAGAGATCGTGAAGCAAAAATTGAACCTATAATCATCAAGAAGTGTGAACGTGATATTTCTGAATTAGTTGATATGATATTTGCTTTATATTCTAGAGGAATGTCAACAAGAGATGTAAATGATTTTATGTTTAGCAAATATGGTGTTAATTATTCTCCAGCACAAATAAGTCAATTAACTAACGAAATTGTCGAAGACGCAAGATTATGACAAGAAAGAAAACTTGAAACATATTATTCAATAATTTATATTGATGCAGTACATTTTCATGTTGTAGATAATAATGTTGTAACTAAGAAAGCTACATATGTTATTATGGGTATTAATAGTGATGGGCAAAAAGAAATTCTAGGACTTTATATTGGAGAAAATGAATCGACAAAGTTTTGAATGTCTGTCCTAAATGCTCTTAAAAATAGAGGAATTAGTAAAATTGATATTATTTGCTCAGATAATTTAAAAGGTATACAACAATCTATAGAAGTTGTTTTTCCTAATAGTAAGCAACAACGTTGCATTGTACATATGATTAGAAATTCTGTTAAATGATATGAAAGAATTTTGCAAAGATTTAAAATCTGTATATCAATCTAATGATGCAAAAAATGCAATGGATAATTTGGATATTTTTGAAGATAAATGAGGCAAAAAATATCCTACATCTATTTCAATATGAAGACAAAACTGATCTGAAATTTCTACAATGTTTGATTACTCCCTTGAAATAAGAACGCTCATTTATACCACTAATCCAATCGAAAATTTAAATAGTGTATTTAGAAAATATGCAAAAACAAAAAAGGTTTTCCCATCTGATGATAGTCTTTTAAAAATTCTTTTTCTAGCATCTCAACAAATCATAAAAAAATGAAGTAATTCAAGAATTAGAAATTGATCTAGCATATTAAATGAATTTAAAATTATTGATTTTGAAAATGAAGAATAAAATTTTTTTCAAAATTTTAAAAATCTTTAATGGTATGTCTTACTTTTATAAGTTATAGTATTTTTAGAAAATAATTTTTGCCTTTTTTTGGTACTTTTTATGGTTATTTTTAAGACATAATTTTCCTTATGTCCCGAAAATTTAATTTTTTTATGTTAAATATAAGGAGACAACTATGAAAAATAGCACAAAAAAAATTGTAGCTTTAACAATGGGTATTTCTGCTGGGGTTGTTGGCGCAGTATCAGCCGGTACTTTAATTGGTTATGCAGTTGACCATTCAAACTATAAAAAAGAAAAATTGTGAATTGAAAACAGCATAAAATACTTTAAAGAATCAAATCAAAAATTAAAAGAAAAAGACAACAAATTAAAAGAAATTTGAAAGAAATATGAGAATCAAGAATGACTTTCTAGTCTTTGAAATGAACTAAGTTTGTGCCAAACTCAAATTGATTCAAATGAAAAACTTATTAAAGATTTACAAGGAATTATTGATCAACACTCTTCATATCATGTTGGCCGCCACTTTGGTAACAAAAGTTATATAAGAGCTGCTAAACACAGTAATGAAAAGAAAATAATTCAAAATCTTTTACCAGAGATGATAGAAAGAACAAAACAAGTTATTAATCAAAGCGAATATGAAAATATCAAAAAATCAATCCAAGATTTTATTGTTCGTAGTAATGATTTATTAACTTTAAAAACCGCAGATGAAAAATTTGTTGATGCTAATAGTAAAAGTGAATTAGATTCTTTAGAACTCAAAATTAAAGATTATCAAAACATTTTAAGTAAAATCGAAGAAGAAGCACTTTCAAACAACACAAAACCTAACATTTCAACTGAAATGATTGATAAGGCAGCAGATTTTATTAAATCTGATTTTCATGTTTGAAATGACAAAATTATTAAAAAATATGTTAATAAAGATGGTTTAATTAAAAAATTCATGTCAAACATATCTAAAATAGAAGAATTATCAGCAAAAATAGAAGATGCTAATACTATTGCAAGTAATAATTCTCCAATTTTAAATCCTATCAATGCTGTTAAAAAGATTTTATTGTCAAGATTTGACTCTAATAATTATGACGTTATATTAGAAAAAAACGGTGTTTCAGTAAATAGAAGTGTTATTATAGAAGATATTATTAAGGCATGTAAAACATTGGAACAATCATATGGAAATACAAAAGAATATATAAATCGTTATGTTAATACTTTTGTCAAAACAATTGAAAATACAAAATTATTTAACAAATCTCAAATTCTAGACTTAAGCAGCAAATCATTAACATCTATTATTAATGGTCTTAATGAATTTAATTCATTTGTTGAAAGTTCAAATCAAATTTCTTTTGAAAAATTAGACGAATTTATTAACAAAACTCAAGCTATTTTAAATGACGAAAATAATTTGACAACTTCTATTCAATCTGTTATTAAAGAATTTTTAAATGCTTATAATTCAAATGTTGATTCAATTGAAACAACAGTTAGATTAGCTAAACTAAATAACTCAAAACAAATTATTGATGAATTTGTACAAAAATCTAAAATTGAATTAAATTCTCTCAAATGAGACAAGTATATTGAAGGCAGAAACAAATTAATTAATGATTATGTTAATAGCAAAAATGAATTAGATTCAATAAAAAATTGATTTAAAAAAGCCTTTGTTAACATTAAATCAAGATTAAATGAATTTAATTTAGCATTAGAAGCAAACAAAACAACATTAAATGGATTTAATTTATTTGAAACTTCTTATAAATCAATAATTGATATACAAACTAAACTAGATCCTTTATTAAAAGCTAATTTAGATTCTGATATTGACAACAAAATTAATAATATTTCAGCATTCTTTGAAATAATTTCAAATCTTAAAAAATATGAAACAAATTATTTAGAAATATTTAAAATTATTAAAAACGCAACTGATTTAAACTTACAAAAAGTTAATAACTTTGCTTCAAAACCTAAAGAAATTTCAAAACTTATTAGTAATGCAAATCCAAAAATTGTTGATTTCTTATCAAAAGTTTTTGCTAATTTAAAAACATTAAGCCAATTTGAAGATGTTAAAAAAACAATTGAAGATATTGATAATAACAAACCTGATTTATCAACTAAAGAAATATCTTCAATTGAAAACAGTGAAATTCAAAATGACAAAGAAAAATTTAAAGGACTATTTGAAAAAATTAATTTAATCAATAATTTTCTTTCATCAATTAATGAAAAAGATAAACTTTATAGTAATTTCTTAGAAAATGAAGCTCAATTTTGCTTAGAATCATACAATGAAATGGATGCAAAATTAGCAGCAATAGCAAATGTGGATTCTAAAGTATATGAATATAAAAACAAAATTTTTGATGCTGCAATTGAAACTTATAAAAATGTTAATTCTACAGAAAACTTTAGTGCTGTTCAAGCTGATAAAGACAACAAAGTTAACTTTGTTAAAAAATGATTTGGTGTTCTTAATTACTATAATGAATATGTAAAAGAAAAAGCAAAAACTGAACAAAAAGCAAAATTAACTTCAGAACAAGAAAGTTCACTTGTTTTTGCAAAAAATGAAATGAATGCAATTAAAAATGATCAAATTTCTAAATTAGATAGTGAAGGTTTAAAAGCTTTGCAACAACTTGATGGTGGCGTTGATTTTACAAACACTAATTTAATTAAAAATTATTTAGAAAATGATTATTTATTAGCTGTTAAAGATTTAAGAGAATATTTCATAAACAAACTAATTTACTTATTACATGAAAAATTCAATTATGGTGATGTATACTGATCTCCAACAAAATTGTATAGACCAACATCTGTCTCACAAGATAATGCAAATTGAGATGAAAAATCAAAAATTTGAACTAAACCTAAAGTTCTAGGCAAAAAACATCTATATGACCATATTTCAGATTTAGATACTGGAGGATACTCAAAAGGTAACACAAATGGAGGCTGAGGAAATTCTAAAGGTGATAAAGTTAGAGCCGAATATTCATGACACCGATATGGAGTAGATAAAGGAAATAGTACTAGTGAAATCGAACAAGGTAAGCAAATTGAAAATCAATATTGAAGGCCTTGACATACAATTATTGAACCTCCAGAAGATAATAATCTACCACTAGATTATAGTATACCAACAGTTAAATATATAAAAGAAGCATATCCTAATAAAAATAATGGCGATTATTTAAAAGCAATACAAAATGATATTTGAAGTGGACATGATGATCTATATACTTTAGCCAAATTAATAGAATATGAGAAAACAATAACTGAAAAAATGCTTTCAAATATCAAAAAAATGTCAGATTCTAAAATAATGTTTAAAGATGATTTTCAAGGCAATTTTGTTAATTTAAATGATGCTCTTTACAAAATAAAATATGCTTTTGATTTTATTAAATACAACAATCCAACAAGCAATGGATATTATGAAGGAATATGAGAAAGATGAAGTGGAGAAGAAAACAAATCTCATTACGCAATTGGAAATAATCCATTAAGAAATAGAGTTAATGTTTATAACAAAAAAACAGGAGAAGAATATCCTATTGGAGTATTACCAAGTAATTTCTATGGTCCTCACTATATAACAAAACGTGGAAAATCAGATGAAAAATATGATAGTACTAACCCACTATTTGAATATATGCCATTTTCTAATACATTATTCTCATATTTAAGAACAGTAGAACATAATTCAGCTTTCATATTACAACATAATGGAGCTCCTATTTCATGAGTAAATTACATTTTTAAATTTACTCTTGATAACCCATTAGGAAACTTTGATGCTTCATACTACATATGAAATAAAATGTACTGAAAATCAGAATATGCAATCACCGAAAGATACATTTTGAAAAATAAATCTTCTGAAACAGTTTCAATTTGAGATAAATGATCAAAAATCTTTACTGAAACAAGTAATAATACATACAGCAAACTTATTTGATAAATTAACGAAATTTAATATTATGCTGAACCCCAAATTTCTTTTTCAAAAAGGGGTTCATTTATTATTTAAATTAAAAAATATTATTAAGACTATTTATAATTTTGTGTAAAGTCTCTCACTTTCTCAATAGAAAAGAATTGACTTTACATCAACACCTAATAATATTGTAGAACAAAATAAAATAATGCTCAAAAAACATTATCTTATTTGGTATGAACCCTTTTCCAAAAAGTCCGGAATTTGGGGTTCACTATAATTTTTAAACTTCGAATTTTATTTTTTTAATTTGTTAAAATTAAGATATGAAAAATTTAAAGTCAAAGTTACTTCAAAAAAATATTTAAAATTTTTACCTGAAAATGAAACGTATAGATTCATAGTCGATTATATCGACAATAAAACAGAAATTTTTTTAAACAACTCTTCAATTGAATTAACTGAAAAAATGTTTGTTTCATAGCTAACATTAACTCGTTTTGCTCGAGCAGCTAGTTTTATTAGTTTTAAATCTTTACAAATCTATATTACTAAAAGAACAATAGTAACAGATGATTGAACAAGAATCAATGTTGGTTCAAATTTAAGTATGCATGAAATAAGTAAAAACATCTTTGCTTACTATACTTCAAGCTTACAAAAAACTTATGAGTCAATTAACAAAGAAGAAATTAAAGAGTATTGCAATTTATTAAGCGAAACTAAAAATCACATATTTTTTGGTATTGGGCAAAGTGAAAAAGTAGCTAGTTATTTACGTGAAAATTTAAACAAAATTAGACTTACTTCATTACCTATTAACAATATGCATGATTTTTTTAACATAGTTTATGTTTAATGGGTCCAGATGCTTTTGTTACAGTTATTTCGCATTCTTTTGAAACAGCTGAAGTTATAAAAGTTTGTGAAATTTTGGAAAACAAGAACATTAATTATGCTTTATGAACTCAAAGCACAAGTATTGCAAAATTAAATGCTTTAAGTATTTTAAATTTAACAAATTTAGCTCAACATATTAAAACTTCTGCTTCATTAGGCTCAAAAATTAGTGTTTACTTTTTAACAGATTTAATCTACTTTTATTTAATCTTTAGTTTAGATCCTCAACTTGATAAATTTTATAAGATTAATTTTAAAAGAGATTTCTGAAATAGTCAACAAAGAAAAAAATATTCAAAAACCAGTGAAAAAACAGTTAAAATTATTAAAAAAAGGCAAAAAAGTCAGTAACTTAGAAAATCCTTTTAATAAATATTAATTTATTAGATTAATGCAACTAGTTTGCATTTTTCTTTTTTAATTTTTGACATTTTTCAGCCAACTTATTTTTTTAATATATATATTATTTATTAAAAATGCAAACAATATATTTAATATATAGTATTATTATTTAGATTAATATATAAGGAGGAAAAAAGATGTTAAAAGTTTTAGATCATCCGCTTATTTCAATTAAGCTGACTAATATGAGAGATAAAGAAGCAAATCACTCTCGTTTTAGAAGAAACTTAAATGAAATCGCATCATTAATGGTGTATGAAGTTTTAAGAAACTATAAAACTAAAGCTAGAAAAATAGTGACACCTTTAAATCAAGAATATGTAGGAGCTACTTTTGATAAAGAAATAGTTATTGTGCCAATTTTAAGAGCTGGGCTTGGAATGTTAGATGGCTTACTTGAATTAATGCCTGAAGCTAGAGTTGGTCACATTGGACTTTACCGCGATGAAGTAACTCATCAAGCTCATGAGTATTTTTACAAAATGCCAGAAGTTAAAAAAGATAGCTACATTTTTGTAGTTGATCCAATGCTCGCTACTGGCGGTAGCGCAGTTGATGCTATTAAAAAATTAAAATCAGATGGCTTTACTAATATTCATTTAGTGTGCTTAGTTGGAGTTAAAGAAGGAGTAGCAGCTGTTGAAAAGGCTTTTGGTAAAGACTTTGAAATTTATTTAGCTGCTCTAGACAAAAAACTCAATAATCAAAAATATATTGAACCTGGTTTAGGCGACGCAGGAGACCGTATTTTTGGAACAAAATAAAAGTATTTATTATGGCAAAAGTATTGTTAAAAAAATAGTCCAAACTGGAGTTTTATTAGCTTTAGCAATTGTAGTTAATATTACTTGCTCAAGCTTTATGAAATTTCCTTTAGCGCCTTTTTTAAAGTTTGATTTTGCACTTGTTGTCGTTACTTTTAGCGCTTTAGCAATTGCACCTTGATCAGCTTTAGTCTTAATATTACTTTTAGCAATAATAGGCCCTGCCTATGGCAGCAATTGTTATGATCCTATTGGTTTATTAGGACATTTCCTTTTAGCTTTAACTCAGCTAACTTTTGTATTAACTTTTTATTTTCTATATAAGTTTTTTAATAAAACAATTTATCAAAATTCAATTCGAAAACAAATAGATTTTTATATTGCTGAATTATCTTTAGCAGCAGTCATTACAACTTTGTTTTTAACTTTTGTTAACGTTTTTATTACAACACCTTGATACTTTAAAGCTTTTGGCGCATTAAAAAATGAACCTGCCACAATTAATTCAATGATAAAAAATTGAGACAAATTCAAAGGTCTATTTCTTAATATAAACAACTATTATTTAGGCACTAGCACTATTTTTGCTCTCTTCAATATTATTAACTTAAGTTTAAATGCTTTAATAATTTTTATCTTTTTAGCCTTCAATATTAAAGCTAAACTTTGAAAATCCGATTTACTTGAAATCAATAAAAAGAAACATTTTAAAAATATTTTTATTCAAGCTTGTTATGCAAAATTAAATGATAATCACAAATAATTTAGACATCATTTTAGGAGTCAAAATGTCAACATATAATCAACATAAAGTCGAAAAAAAATGACAAAATTATTGAGCTGAAACTCAATATTTTGAACCACAAAAAACAACCAAGAAACCAAAGAAATATATCTTAAGTATGTTCCCTTATCCAAGTGGAAATATTCATATGGGGCATGTTAGAAACTATGCAATCGGCGATGTTTTAGCACGTTATTATCGTCGTAAAGGTTTTAATGTTTTACATCCTTTTGGTTGAGATGCTTTTGGTTTACCAGCTGAAAATGCAGCTATTAAAAATGGCATTCACCCTAAAGATTGAACATACAAAAACATTAAAAATATGAACCCTAAATTAAAGGATTTGGGAATCTCTTTTGCTTGAAATTATGAATGTATTACAGCTGATGAAATTTATACAAAATGAGAACAATTGATTTTCATTAAGATGTGAAAAAAAGGTTTAGTATATCGTAAAAAAGCATTATTAAATTGATGCGAAAATGATCAAACAGTTTTAGCAAATGAACAAGTTGAAGATGGAAAATGTTGACGTTGTGGCAATATTGTTGTTCAAAAAGAAATGGAACAATATTACTTAAAAATTAGAAACTACGCAGAAGAATTACAAAATGATTTAGAGTTATTAAAAAATCATTGACCTGACAAAGTTTTAATGATGCAAAAAAACTGAATTGGCTATGAAACAGGCTTTTTGGCAAAATTTGACATAGTTGAAAATAATCAAAATCACTTTAGTAATATTGATATTTTTACAAAAAACAAAGATATTTTAGTAACCATGAATTTTATAACTATTGGTGCAAATCATGGCCTTGTTAAACAATTAATTGAAGAAAATAAATTAAGTGATAAAGAAATTGAAAAACTTAATTTAATTAAAGCAAAAGCTACAGCAAAAGATTTTTCGCAAAAATTATGTTTAGCTTTACCACTTCAAGCAATTAATCCAGTAAATAATCAAAAATACAAAATTTATGTTAGTGATTTTTGTTCTCTCGGAGAAAAAAATAGAAGTCAAATTATCGATACTACTAAAGTTAAAAGTCATGAAGAATTTGCCAAATTTAATAACTTAGAAATTACTAATTATGCAAATAACAAAGCTGATTTTAGTAAGCTTGAAAACTCGAGTAAAATCAACTTACAAGATTGAGGAATTAGTCGCCAAAGATATTGAGGCGCTCCTATTCCTATGATCAATTGTGCTAAGTGTGGTTTAGTTCCTGAAAAAGAAGAAAACTTACCAGTTACTTTGCCTTACAAAGTCAACTTTACTACTAGTGGCAACCCACTTAAAACAAATAATAAATGATTGGAAACTAAGTGCCCTAAATGTCACTCAAAAGCAACCAGAGAAACTGATACTTTTGATACCTTCTTTGAATCAAGCTGATACTTTTTAAGATATACGACACCACCAGCTAAAAGAGAAAGCCATATTTTTGATAAAGTTCAATTAAAATACTGAAATAATGTTGATGAATACATCGGTGGAGTTGAACATGCTATTTTGCACTTACTGTATGCACGTTTCTTTACTAAAGTTTTAGCAGACTTAAAAATGGTTGACTTTAGAGAACCTTTTGATAATTTATTAACTCAAGGAATGATTCTTAAAGATGGACTTAAGATGTCTAAATCAAAAGGTAATGTTGTTAGCCCAGATGAAATGATTAAAAAATATGATGCTGATACTATGAGGCTTTTTGTTCTTTTTGCAGCACCGCCTGAAAAAGAACTTGAATGAATTTCCTCAGGTATTGAAGGTTGTCATAAATTTATTAAAAGATTAATAGCAAGAAGAAAAGAAGTTAAGGCTAAAGACAAGTTAGCTGATATTAAAAATCTAAAATTAAACAATGAAGAAAAGAATGCTAGAAGAAAACTTTATCAAGGTTTAATTAAGCAAGAAAACTTGTTTAGTGATAGACGTAATAATTATGCTTTTAATACTATAATTGCTTGAGCTATGGAAACTTTCAATGCTTATGATAAGATTACTAACTCATTATTAATTAAAGAAATGTACTATGTTTTATTAAACATTTTGGAACCATTTATTCCACATTTAGCTTGAGAATTAAGTGAGCAATTCTTTAAGCTTAAAAACCTTAAGGACTTTAGTGTTGATAATAGTGCTTTAGAACATGATGAAATCACTTATGGAGTAACAATTAATGGCAAAATGAGAGTTCAAATTACAGTTGACTTAGCAAACAATAACAAAGATTATGTTTTAAATTTAGCTAAAAATGAAGCTGCTAAATGATTAGAAAATCAAGTTGTTATTAAAGAAATTTTTGTGCCAAATAAAATTGTTAACCTAGTTATAAAGGCTAAATAGAGCCAAAAATCACAAATAATTCCTAATTTTATTTATTTATTAAATAAATGTTAAGAATAGATTAAGAAAATATAAAAATATTTGGCACTTTAGGCATAAGAGTGCTAAAATATAAATTATGTATTTTAGAAGGAGACCATATGAAAGAAAAAATCTTACTGTTGGACGTCTTAGATAAAGAATATTTATCTGACGCAGTTTATAATCATGAAACAAAAATTTTAGAAATAAAAACAAAAAACCAAAGTTTTAGTGATCAATATTTAAATCACTTGTTTAATTATTTTGAATCAATTAGTCATGATGAAAAGAAAGAAAAAAATGACCAAGCTAAATATTTATTTATTGGTTATTTTGACTACGCAAAAAACAAATATGATGGCAGCGATGAAAGCAGAAAAAAAATAGTTACAAAAAGTAACAATGTAAACAAATATTGTTCTTATGTTAGAGTGCTTGATTTAAATATTGAATACAAACAAGATTCAAAAGAAATCGATAGATATAAACTTAAAGTAAAAGGAATTGGACGTTATAAAATTACTCATTTAGAAGATAATAGTGAAAAAACACCTGAAAATTCATATAATGTTGAATTTGCAGTTGCTTCAAAAAGTTCTTATGAAAATAAATTAAGTAAAGATTCAGTTAAAAAATTAAATGTATTAGATAAAACTATTACAAACTTAGTTGAAAAAGGATTATGAAGCAAAATTTTAGATCATAATTTAAGCTTAAAAGAATCAGAAGGAGCACAAAGTAAATGAATGGAAGAAAGCATGTTTAACACGCCTCGTTGAGATGAAGATTATGATGAATCTGGCGAAAATATTGTTGAAGCCCTTTTAATTTATTTTGCTTTAAATAGCCTTTATAAGATGGATATCTTTAACTTAGATTCAGTTAATGACCATATTGATTACTTGTCATTATTACTTTATTCATATCGTAATTTTGGTTGAACTCCAAAAGAAAAAGGGATCATTAATTCACTTAAATTTTTTGACGAAAACTATACTAAAACAAAAAGCATTAACACAAATTCAAAAGCAGCTGTAAAATACTTTGAAACAGAAGCAATTTTTAGAGCTAATGAAATTAAAGAAGTTGCAAAAGAATACAACGAAGAAAATGAAAAAACTAAAAAAGTTCAAGAAAACAAAAAAAACTTATTAACTAAAAAACCTAAGAGTGAAAAAGATTCTAAAGATCCTATTGCTGAAGCTTTATTAGAAAATGAAATAAACCAAAAAGTTAAAAGTAATTTAGAAAAACAACAAAAAGAATTTCTTTTAAGAGAAAAACTTAAAGCTATTAAAGAAAGTCTTTCAGAAAATGATTCAGAAGGTGATGAAGAAGATGACTCATTTTCAAAACTTATTCATGATCCTAAATTAAAACAAATTTATCCTGAAAGTGTTTTAAAAATTATCTCTACAGAAAAGGAAAGACTTAAAGGTATGATGTCAGGAACACCAGATGCTAATGTTGCTGCTACTTACATCAGCACATTAAAGAAAATGCCTTGAAGAAAAATAGAAGTTGAAACTTTAGACATCAAACGTGCCAAAGAAGTTTTAGATAAAAATCACTACGGCTTAGAAAAAGTTAAAGAACGTATTATTGAATACTTATCATTAATTATTAACCATAAAAACTTATCTAAAAAATCAGACCAAGACAAATTAATTGATATTGACAAAAATTATCAAATTGATATGTCCTTATTTAAAGAAGATGAAAAAAATGCTAAATTACAAACAGAATTTAACAATGTTCCTATTCTTACTTTAGTTGGTCCTCCAGGTACTGGTAAAACCTCTTTAGCCAGAGCTATTGCTGAAGCGCTTAACAAAAGTTACATTAAAATTAGTCTTGGTGGTGTGCATGATGAAAGTGAAATTAGAGGTCACAGACGTACTTATGTAGGTTCAATGCCAGGTAAAATTATTAAAGCTATCCAAAGAGCTGGTGTATCTAACCCACTTATTTTATTGGATGAAATTGACAAAATGTCATCTGATTATAAAGGTGATCCAGCTAGTGCAATGCTTGAAGTATTAGATCCTGAACAAAACACAAAATTCCAAGATAACTATCTTGAACATGAATATGACTTATCAAAAGTTATGTTTATTGCAACTGCTAACTACTACGAAAATATTCCTGCTCCTTTAATTGACCGTGTGGAAATTATCGAATTAAACTCATATACAATTAATGAAAAAATTAAAATTGCACGTGAACACTTAATTGATTCTACAATTCAACAAGCAGGTCTTAAAAAAGAACAATTCAATATTGATGATAAAACACTAGAATTTATTATCAAACACTATACAGCTGAAGCTGGTGTTCGTGGACTTAAGAGAGATTTAGATAAAATTGCTCGTAAAATAGTTACAAGAATTGTTGAAGGTGAAAAAATTGCTAAATTTACAATAACAAAAGATATTGTTACTGAAATGTTAGGTACTCCTAAATTTACAGATCACCAAGATACGAAAGAACCTCAAGTTGGAGTTGTTAACGGTTTAGCTTACACTTCAATAGGTGGAACAACATTGCAAATCGAAGTTTCAAGCTTCAAGAGTAAAAATAGCGGTATCAAATTAACCGGTTCGCTTAAAGATGTTATGAAGGAATCAGCCAATATTGCTCTTTCATATGTAAGAGCTAATGCTGAAAAATTTGATATTAAAGATTTTGCTTTTGATGAAAGAGAAATTCATATTCACGTTCCAGAAGGAGCTGTTCCTAAAGATGGGCCTAGTGCTGGTGTAACATTTACAACTGCTTTAGTTAGTGCTTTAAGTAATAAACCTGTACCTCAAGATATTGCAATGACAGGTGAAATTACTTTAAGAGGTCGTGTTTTTGAAATTGGTGGATTAAAAGAAAAATCATTTGCTGCATATAAAAAAGGAATCAAAACTGTATTTATTCCAAAAGACAATGAAAAGAATTTAGCAGATATTCCTGAAGAAGTTAAAAAAGCAATTACCTTTATTCCAGTTGAAACTTATGAAGAAATTTATAATCACATTTTTAAAGATCAAAAGCCTACAACTGATAAAAGCGATTCGACTAAAAAGTCTATAAAACAGGCAAATAAGAAATAATTTAAATGACTTATATTCGTTGAATATAAGTTTTTTTAATACCCTCATATTTTTTAAAAAGTAACAACTAATGTATAATATTTAAGATGAAAGAAATGCAAATTATAAGTTATGTTTTATTATCAATAACTTTATTATTAGCTATTATCGGAACAATTTTTGGAACAAATTTTTATTGAAAAACTCCTAAGCTTAATAAAGTTTTTAGCTATAGAACAAAATTATCATTAAAAAATCATGAATACTTTTATTATGCAAATCACACTTTTGGAAGAGGTTTGTTTTCTCAAAGTATAGTCATTTTAATATTGTCTTTTATTTCTTGCAGCATTGTTTTTTCACTTAAAGATTATCATTATTTATTCATAATAATTTTTGCAATTTGATTTTTGAGTTTCTTTTCAATCATTATTTACATTGAATTAAAACTTAAAAAAAGAGACAAAATAATTACAGATTTATAAAAAATCCATAAAAAATAGAAGCATGTAACAATGCTTCTAATATTTATTTTTAATGCGTTCGTAATTCTTTTTGTTTTTGTTGTAATAAGCTTCTAAAAGATCTTTTTGACTGTAACCTAATAATTTAGCAACCCCTAAAGTTAAAGCTAATATTTCTCCAATAACATATTTATTGATGTTATTTGTTGCTGAAGAAATTGTTTGGAATAATAAGCAAATTTGTTTATTAACATCCTTACTCATTATTAATGGTGTGATGTTAGGATCAACTTCATATTGATAAGCAAAAGAGCCAACAAAGTGCAATATATCAGCAAATTCTTCTAAAGCTTTGTCTTTATCAATTTGTTTTTTTGCTTTTCAATATTTGAATGTTTGGACTTCATTTGCAAATTCTGCCGTTTCAACAAGTAAAGCTAATAATTTCATGTAGTGAAATTCTTTAATTTTGCTTTTTGAAGTCTTTGCATCAATATCATTACGAGCTGCAATAGCTTTGTCAAGATCTTTTTGCATTGTAAATATTTTTGTTAAATTCATGTTTCAATTATACAACAAGTTTAAAAGCGCAATAAAAAAGCTAAATTATTATTTAGCTAAATATTTGCTTCCTTTAATATATCTAAAGAGTGAGTTGCTTCTTTTGATAAAGTATCTTTGCTATTTAAATATGAGTTAAGTATTTCTTTACTTGCTTCAAAATTAAAAGCTGATAATAATGTTAAAAATTTCCAAACAAAAATTTTATTATCAATATTTTGACCATAAACATCAATTAGCTCATTTAAAAGTTTTTCAGTAGCTAATTCTTTTTTATTAAAACAAATGTAACCAATTGCATCAATAACTTCTTGTTTTTGTTCAATTGTTCCTTGCTTAAGAGTTTCAAGTAAAAGAGGAAAAATTGAAGTATGCATTTTAGCTAAAGATTTGGCAATAATGTCTCTTGGTAGTGGATAAGAACTTTTATTACTAATATATTTAGGCAATTCTTTAATTTGGTTATTACCAATTTTGCTCAAATATTCAATCATTTTATTAGCTGTAAAAGCATTACCTGTTTCTAAAGTTTCACAAATAGCGATTTTAGTATAAAGTTTGTCTTCTTTTTCTAATCGTTTTAATAATGCATCTACACTTTCTTCATCCATAGGAGATAAATTATAAGCCGCAGAAGATCTAACTCATGCATATTCATGTTGCAATAATTCTAGTAATTCTTTTTTGCTTTTGTTTTCAAATTCTGCAATATCTTCTTGATCAACATAACCTCTTGATAATCATTGTTTTTTATTTTCTACTGGTTTCATAAGTATCCTTTTGATATAAAAATGAGCCTTAATTTGGCTCATATATTATATAATTTTATTATTATTTTTCTTCTTGTTTTTCTACTTCTTTTTTAACTACAATAACTTCATCTTTGAAGAATTGTACATTTGCTTTTTTTCTAAGTAGTATAGCAGCAACAAAGGAAACAATTCAAATAAAGCAACCAATTATAAATAAAATCATAAATGTCTTATCATTTCATTCTGAAGTGTAAATTGCTAAGAAAATTGAAAATACTGTTATTGTAACTCAAACTGCACCTAGTGCTAATCCAACAATAAGTGTAAATAATGAAAAAGTTAGTGCAGATGATCGAGTATAACCATTAGTCGCAATAACAATACTTTGAATAATAGCTAAAAGAATTAAAAATCCAATAAAGCTACATGCAATAATACTGCAAATAACTATTCATTTAGTATAGCTTAATTCTTTTGCTTCCAATTTTTCATTGTTTACTTTGTTTAAAGATTCTGTTTTGTTTTCTGTTGACATAGATCTCCTTTTCATTTAAACTCAAAAACATCTATTTTCCGATGCAATTTAATTTTATATAAATTTAAACAAAATAAAAAAATTTTTTAAAAAAAGACACTCACATAAGAATGTCTTTAAAATTAATTCTATTCTGCTTTTGAGTCAATTGATTCATCTGATTTAAAATAATTGAAGTAGTTTTTATTTTCTTTTTGAGCATCAGAAATATAAATAAATGCTCCGATGAATGTTAATATTCCAACGAAGAATCCTATAATTAAGATTATCATTGATTTTTTATCTTCTAATTTGCTTGCTTGAATAGTTATAACAATATGTAATACAAATGAAGCAACGCTTATAGGAAGTATTGATAAAATTAATGCAGTAGGGATAATAATTTCTTTTCAGTTATTTTTAATATATTCATTTGTTTCTTCTCCACTTTTAAATATTTTAACTGCAACATCAATAAACATAAATATTGCAATTCCTGTTATAACAGCGCTTAAAAGTGATAAAACAATCAAAGCAATAGCTGAACTTTTAGGATTTTTTCTATGCATTTTTCTCCTTTAAAAATGTAATTTTATTACCTTAGTATTATAAAAAATAAATTTTAATTTTGAAGAAATATTTTGTTAAATGTTTCAATATGGTTCATTAATATGAACTAATTCAATTTTAACTCCAGGGAATTTTTCTTTGATTATTTTATATGCATCATCTGCGAAAACTTGTTCATCAAGGTGAGAAATTTCTAAAATATTGGCTTTGGTTTCTTTATAAACTATTCAGTCACTTCATTTAATATCGCTTGTAATAATAAGATCATAATTTAAATTATTTATAGCATTAACTTCTGAAATATATCCAGCCCCTGAAAGGAAAGCAATTTTACTAATTTCATTGTTTCATAGGTCTTTATGTAAATTTGTACGCATAGCTTTGAAGCCAAAAGTTGTTTTAATTTTTTTAACTAAATCATTAAAAGTAGTTTTGTAATTTACTAAATTAGGATAATTATGTTCATAATTTTGGCGATAATTTTCAAGTCCTAATTTTTTTATGATTTGATATGAAGTTCCATAGTATTGGTTGTCATAGTTAGTATGAAAAGCTAAAACATTAATTCTTTTTTCTTTGCATTTTTGATAAATTGATCTTTTGTATGGAGCTTTGATATCTTCATCATCTCAAGATGCTTCAAATTTAAAAGGGTGATGAATGATGATTAAATTAGCATCTATTTCTAAAGCTTTTTCGAAAACTTCTTTGGTTAAGTCAATAGCACAAACTATACTACGTAAAGGTTCTGATAAATTGAATTTAATAGAAAAACCACTTGGGTCTCATTCTTCTTTAAGATCAAGTGGTCACTTTTCTAATAAATATTTTGTTACTTTTCTAATTTGCATTATTTAATGAAATCTTTCAAGTGACGTCCTTTACGTGTGCTACTTTTAAGTTTTCTTAAAATTTTGTTTTCAATTTGACGAATTCTTTCTTTAGAAACATTTCTTTCTTCTGCTAATTTTTCAAGTGAGTGAATTGGGTATTTATTTCCATTTTCGTCAACTCCAACTCCGAAACGTTTGCAGATTAAAATTCTTTCATCATCATCAAGTGATTTTTCAAGAATTGAATTTAGTGTTTCCACTAATTCTTCTTGTGAAGCATAATCAACTGGATTAACAACACTTTCATCTTTAACAAAGTCACTAAATGATGAATCATTTTCTTTACCAATTTGTTTGTCTAAAGAAATAGGGTCAATGTTGATTTTTCTAATGTATCTAACTTTTTCAGCGTCATAGCCATTGCCATATCTTTCAGCAATCATTTCATCAGTTGGTTCATAACCTAATTCTTGTTGCAATTCACGTTCAATTTTAGTGATTTTGTTAATAGTTTCAACCATGTGAACAGGTACTCTGATAGTTCTAGCTTGATCAGCTACAGCGCGAGTTATTGCTTGACGAATTCATCAAGTTGCATATGTTGAGAATTTAAAGCCTTTTGAAACGTTATATTTTGAAACTGCTTTTACGATACCTGAGTTACCTTCTGAGATTAAGTCAATAAAGCTAAGTCCTCTATTTTTGTATTTTTTAGCATTATTGATAACTAAACGTAAATTACGTTTGATAAGTTTATCCCTAGCACGTTTTCCTCTAAAGCCACCTTTTTCCATTTCTTGAGCAAGTTTCTTTTCTTCTTCAATGGTTAAAAGTTTTCCATATTTACCAATTCAACGCATGTATCATTTAACAATATCATTAGTTTCGGTAAGTTTGTTGGTTAAATCTTTATCTTTAATTACTTTGTTATCATTAAGGTTAATTTCTTCATTTTCATAAGATTCAAGTTCAAGTGAATCTTCATCTTCATTATTTTTACTTTTTTTCGATTTTTTCTTACTGCTTAATTCTTCTTCGTCTTCATCTTCGTCATCTTCATCATAACTTGAATCAACATAGTCATCTTCAATAATGTATTCTTGACTTTCATCATCGTCACCATATGAACCAACTAAATTGTAGTTATCATCTTCATCTAAATCTTCGTCATATAAATCACTTTTTTTGTTTTTAACATCCATTTCATCGGTGTTTTCGTCTTTTTTGCTTTTTTCTTCGAATTCTAATTCTGAGTCTAATTCATCTAAACTAACATCGTCTAAATCGCCCTCGTCGCTTTCATCAAGAATTAAGCCTGATTCAAAAAGTTGGCCTAAAACTTCGTCCATTGATTCATCATCAATTTCAACTTTAATTGAATCTAAATAATCATAAACTTCAGACTGACTTATTTGTTTTTTCTTTAAGCTTTTTGCATGTTTTTTAATCATTGAGATTATTGATTTAATTTCATTTGCTTTATTCATTTTTTTCCTTTCTTTTTTGCATTTCTCTTAATTTTTCTTTGTGTTCATCATACATTTTTTTGATACTGCTGATTTGTTTCAAAACTTCAGGATTGCTTGTGTATTTATTATAAGCATTATCTGATTCCTTTAATGCTCGGCGATAAACATCTTTAAAGTTTTGTTTAATAATTTTATCCTCTTTGTATTTTTTTGAAAATTGATTTAAATCGATATCAACATTTTCCTGTTCTAAGTCTTTGATATATTCCTTTAATTTAGCAACCTTAATACTTTTACTTTCTCTAAATGTTTGATAAAGTTCTTTTTTTAAATCTTCATTATTTAATTTACCGAAAAACATTAAGTCTTTGTGTGTATCTTCTCTTTCTTTAAATAGCTTAATTAATTCAGTGTGATTAAGTAATAAATAGAAAAGATTGTTAATTCAATAATCGCTTAATGGCACTTCGTTATTTAAAGGGTGATTAAAATTATTGACATTATTGCTGTATTCAACTACTTGATTATAATTTTGTTCATCATAAGCATAAGGGGGAATATTGTTTTCTAATAATTGATATTCACCTGAGTCTTCTGGAAATTGTTGTGGTGCATAAATTTCTTGAGAATCATAAGGATCAGGCAACTTAAAGTTGTGTTTTACTTGAATGCTAAAACCATAATCGGTTTGAATTTTGTTTTTGTAGAATTCTTGAACATTAATTTCTTGGTGTTCAAGGAAGTTAACAAAGTCATTGCAAAAAGCTTTAACATTTTTGATTTCATTGTTTGGCGAAACACTGAGATTATATTTCTTGCTTAAATTTTCATAAATAAAATCTAATGCAGCTGGAGCTTCTTTAATTAACTTGTGAAGAATATCGGCACCATAACTATTAAATAATTCATCGGGGTCATATTTAGTTGTGTTACGTACTATGTAAGTGTCAATACGATGGTCTAATAAAAACTTAGCACTTTTTAGTGAAGCTTTTTGACCAGCATCATCACCATCTAAAAAGATGACTACTTTTTTGTTTTTTAGTAATCGGAAATGATTATTGGTTAAAGCTGTACCCATTAAACCAACAGCATTATAGATTTCAGCTTTAAAAAGTGCAATAACATCAAAAAATCCTTCAGTAATTATGATTGAATTGTCTTCGCTTTTACTATTAGCTTGTCAATAGTTATATAAAAGAGAATTCTTTTTGAAAAGTTTAGTTTCATTACTATTGATATATTTAGGTTTAGTATCATTGTCTAAAGCACGAGCACTAAATCCAACTATTTCACCTTGTTCATCTTTAATAGCAAAAGTAATTCTATTCTTAAATGCGACACTTTTGTTATGACTATTAATTAAACTTGCTTTGACTAATAAGTCAGGAATATTTTCTAACTCATTTGCAAAAATTGAATTGAAACTTTCTTCTTCACTATAACCAATATCAAAAGCCTGAATGATTTCACTAGTTAATGCTCTTTTATGAATAAAATCTTTTAATTCTTTGTTTTTTCTTTTTATTAATTCTAGTTTAAAAAGTGAATTAGCTTTGTTGTTGATTTCAATTATTTTTAAGTCTTCTTCATTGTAACGACTTATTGATTTTTGGAATGAACTTAAGTCAACATTGATATTACTACGATTAGCTAAATATTCTAGTGATTCTTGGAAAGTTCATTTTTTGTATTTCATTAAAAATGTTAAAACATTACCACCAACTTGGCAGACAAAGCATTTAAATATTTGCTTTTTAGGGTCGACATTCATTGATGGATTTGTGTCTTGGTGAAAAGGACAAATGCAAACAAAACTACGACCTTTTTTGCTTAAAGTAACAAATGAGTTGATAACATCAACAATATCATTACTTGCAATAATTGAGTCAACTATTTCATGGTTATTATTACTCATTTGTTACCTCCGTTTTTTTCTAAAACTATTGTTTTATAGTTCTTTTAGTAAATTATTAATTAGTACAGTTTTTTGTTTCATACTGTCTCTGTCTCTAATTGTTACAGTATTTTTTTCAAGTGAATCAAAGTCTATTGTAACACAATAAGGTGTACCAATAGCATCTTGACGACGGTATCTTTTGCCTATTGATCCAGTTTCATCATAAGTTACAGATAAGCCTGCTTTTAGTAATTTTTCATAAACTTCTGTTGCTTTATCATTTAGTTTTTTAACTAAAGGTAAAACTGCAACTTGATAAGGAGCTAAATTTTTGTGTAGATGTAAAACAACTCTTGAGTCATCTTTTTCAAGTTTTTCTTCGTCATATGAATCAATTAAAATTGCTAACATTAAACGGTCAAGTCCTATTGAAGGTTCAATAACAAAAGGAACAAATTTTTCGTTTGTTACAGGGTCAACATATTCTAAGTTTTCACCAGTAGCTTTCATATGAGCATTTAAATCATAGTCAGTACGATTAGCTATTCCTAAAAGTTCACCTCAGCCAAAAGGGAAAAGATATTCAATGTCACTTGTGCCTGAAGAATAATGACTTAGTTCTTCTTTTTCATGTTCTCTGATACGAACATTTTCTTTGCTTAAGCCTAAATATTCAGCAAATTTTTCAGCTTTTTTAATGTAGTATTTGTAAAGTTTGTTTGCTTCTTTTGGATGGCAAAATACTTCAAGTTCCATTTGTTCAAATTCACGAGTACGGAAAATAAAGTTACCTGGTGTTACTTCATTTCTGAAACTTTTTCCAACTTGCCCTATGCCCATAGGTAATTTTGAACGCATGCTTCTTTGTACGTTTTTAAAGTTAACAAAAATACCTTGAGCAGTTTCAGGACGTAAGTAAATTTTGTTTTTGGTTTCTTCTGTAACTCCTTGTTTGGTTTCAAACATTAAGTTAAATTTTCTAATATCGCTTCAGTTAGTTTTAGCACCATCGTATTCTTTAAGATTCTTTTCAAGGAATTTTTTGATTTCATCATTAGTCATTTTTTCTGGAATAATGTTAGGATCAATCTCTTGAACTAATTTGTCAGCACGATATCTTTTGCCATTAACTTTGTTTTCAATTAAAGGATCATTAAAGTTAATAACATGACCTGAAGTTACTCAAACTTGAGGATTCATTAAAATTTTTGAATCTAGTAAATGGTTGTTTTTTTCTTTTTTAATAAATTCGCTTTTTCAAGCAGCTTCAATATTATCCTTTAAAAGTGAACCAAGAGGACCATAGTCTCAAGTATTAGCTAAACCACCATAAATTTCACTACCTTGATAAACAAATCCACTAGCTTTTAAATGATTTATTAAAGTAGTCATATTTTTTTCATTATTCTTCATAAATATCTCTCCTTTTATTAATTGAATAATAGTAAATAATTACAAAAAATGCATCTAAGTGCATTTCTTGATAATTAAATTAAGCACCAAAATTAAAGAATGTATAACCACCTGCTAATTGAATAATAGCAAGAATATCTAAGGTTAAAAGACCTGCAAGAATTAGTACTAATAATAAGATTAAGAAGTAGAATCCTGCTGAACGTTTTTTCTTAGTTGAAACATTGTTTGATGATTCTTCTGATTCTAGAGCAACTGGTTCATTACCTCTAATTCCTGTGTAGAAATTATCTTCAGGTTGAGCTTCTTTTTCTTCAATTACTTGAATAGGTTTTTCTTCAACAACTCTAACAGCAAAGTCTCTTGTTTTAGGTTTTTCAGCTTTTTTATGGTCAATTAAGACAAAATCATTGTCTTCAACTTTTTCTTTTTTAGCTTCTTCAGGAGCTAAAACAATAAGCGGTTCTTCTTTAATTGTTGGTTGTTTTTCTTCAACTGGTTTAACTTCTTCAACTACAGCTTCAGCTTCAACAACTGATTCATCTTTAACTTCTTCAACTACTGGAGGCACTTCAGTAACTATAGTTTCAGTTTTAGGTTGTTTTTTAGTTTTAGAAGATTTTTCAGCAGCTAAAACAGCTTCTTCCATTTCAACTGCATCTCTTTCTGCAGATTGAATTGTTATTGTTTTAGTAGCATCTGATTCATTAATAACTGTTACAGCAAATTTGTTTCTAATTGCTAAATATGGAATTGCATATTCATATTCTTCATAAGGAACAAATCCAACATGATAAAGATCAACTAAAACATATGAAGTAGCTCATGAGATTTCTTGTTTTTGCTTAGTTTCTTTATTTAGGAAATAACCGCGGTTAGCTGGTGTATCTTCTAATTTAGGATCTAAGACTAATAAACTAAGTAATGATTCAAAAGTTGTACTTAATTCAGCATGCATTTTTTGAACTAAATCAGCATGTGGGAAGTTAGGATTTGCTTCAACTTCTAATAATTGTTTATTGAAGTTAGCATAGTTTTTCTTAATATTATTGTAATCAGCTTCACTAATATTTTGTTTTGGAGTTGTTTCAATAATATTTTTAATTTTTTCTGTGTATAAAGCAACACCACCTACATAGTCATCAAAACTTAATGTGATGTAGTTCATGTATGTTACACCTTCAAGTGGCTTGTAGTTTTGTCTTAAAAGTTCCATTTTACGAGCCATTTCATTGAAGTTCATATCTTTTTTTGCTAATTCTTGTTTAAGAAGTTCAAGTTCTTTGTCTGCTTCAGCATTGTTTTCATGAATTTGAGTTAAAAGAATATTAATTTGTTCTTGGAATTGTGCTCTAATAGCATCAACGTCAACAACATCTGATTTCTTTTTCTTTTTAGCAATTTCTAATTCAGGTGGAAAGTAGGTTGCTGGATCAGAAATTAATACAAAATCAAGATTTCTTAATCTTCTTTTTGCATCATAATCATCGTGTTGGAATGTTGAAGGATTAATTCCAATTTGACGACAGTTACCTTCATATGTACCGCCACCATCAAAACCAGCAGTTTTAATGTAGTAGCTTCATTGTCCATCTTCACAATCACAAGTTAATTGACCTTCAAAAATCTTTTGATCATTTTGATATCAAACAGCTGTTTCATACTTAAGTAACATGTATCATTCAAGAGCTTCTTGTCTTGTTTTAAATTTTGCTAATCCAATTTTGATTTTTGGATGTTTTAGTAATCAAGGATAAATTGGGTCCTTTTTAACTTCATATGTACAGTTAAGTCTTTTCATATTGATTCCTCGCTTATTTTTTACTAATTAGCCAATAAATAGTGTATTTATTGAAAATTTTTTTTACTATTTTTGGCCTCAAACAATAGTATTTTATTATTGTATTAATATTATAAATTAATATTTAATTTTATAATGCAATTAGTTCAAAAAAATTAGCTAAAAATAAATATTTTTTATTTTTGACCAAAAAAATTGCTAAAAAATCAAATATTTTTGCATTTTTTCTTATTTTTTTCCATTTAATTATTAATATAAACATAAAGTTTAAGTATAATAAAAACACCATGAATCATAAAGGCGATATTTTATTCGGAAAAATAAAAAGTGTAAGTAGTGAAGGACTTACAATTTTATGCAATAATGATTATTCTTATTTTATTCCTCGTGATTTGATAACTGACTGAAACAAAATTAACTTGCGTCATGAATTTAAGATCGGCGAAAAAATTAATTTTATAGTTGAAAAAGTTGATTATCACAGTCATAGTGGTATTGGTAATTTTAAAATTAATCATGCCACCTTTTCACGCAGTCCATTTAAAGAAAATTTAAGCAATACAAAACATGGTTTTGAAAACTTGAAAAACCAAATTGATCAAGAAATCAAAAATTATCATAAAGAAGGTAATAATGGCAACAATTAATTTGAAAGCTTATAATTATTCACCTGATTTTAAAAATGAAAGTTTGATTAATCGTGGCAATAACTTGATTAAGGATGTTAAATTAAAAAATATCGAGGGTTTTGAACATTTTGGTTTTCATGAATTAGCTTTAAATTTTAGTAATTTTAATGTTGATGAATTAAACAATTTTGTACGCTTAATAAAAAAGCAAGAAATTAAAGAAATTTTGATTATGACAACAAGCAAAATAATTGATAATTATCAAGCTGGAAATGACTTTTTATTTAAGTATGATTTATTAAAAAAATACAAGTTAAAATTTAATTTTATTAATGTTGATAATTATGAGCAAGATTACTATGCTAAGTTTGAAGAGTATAAGAAAAAATTTGAACAAAAAGGCATTGCTTTAATGTTTGGATCATTTACTAAATTTAGTGAATAAACAATTAATTTTATTAAATTATTAATGAATCATTTTCAATTAAATCAAGGTTATTATCGAGCACTTGAAAGAGTTTTTTTAATCTCAAAAGAATCATTAGAAAAACAATTGGAATTTTTAAGTATTCCCCTTCAAAACAGACTTATTAGTCCAAATATCCTCTCAAATAATTTTGCTTTTTTTAGTGAAATTAATATGTTACTTTTGTTGCTTCAAGGAGCTAATATTAATGACTTATCTGAAGGCTATTCTTCAGGTTGCACAAACTGATTAAGTAGTAACTTAGAACAAAACAAAGCATTCCAATATGCCTACATTAGGTATAGCTTAAAAGGTGATCGAAAAAATGATCTTTTAATTGGAACTGATGAAAGATTTAAAGGTATTTTGAAGCTTTATAGCACAATAAATAATAGTCAATATTTAAAGTCAAATAATGTGACAATGTATGCAATTTTTAGTCAAGATATTTATACTTATGGACAATATTTAATTGATAATAATCGCAACTTTTTTACCTCAATTTTTAGCATTGAAAATGAAAAAATTGACTTTAGAATTAGTGATGAAATTAATAATACTCAAGATGGTTTAGAGAACCATAAAGATAACCGCATAAGCAGCTATTACAAAGTAGCAAAAAAAGGTTATTTGACAACACTTTCAAATGTTGCATCATTACCAGTTTTTGATATTTCATTGGAAAGTTCAAATGAATTAAATTTAGGATACTTAGTTGGATTTCTATATTGAAGTTATATTTTTGAATGTTACTTAAATAAATTGAATCCTTTTGATATATAAAAATTGTAATAATTTCCATATTTTTTAATTAAAAGAAGATAGTTAACAAAAGAAAATATTAAAAATGTAGAATAATTAAAGAAATTATTAAGTTAAGGATAAAAAAATATGAAAGCAGTTAAAGTTAATTTACATAATTGTGTTGATGAAAAAACATTACTTTCTTATCAAGACAAAGTTAAACAAATTCATGATTCAATAATGAACAAAACTATTGCAGAAAAAGATTGATTAGGTTGATTAGAATTACCTAACAAAGGCAAAAGTGAAGAAGTTAAAAGAATGAAAAAGATAGCTGAACTATGAGAAAGCAAAAACATTGAAGTAGTTGTAGTTATTGGTATTGGTGGGTCATATTTAGGAGCTAAAAGTGGCTATGAATTTATGTATGGACCTTACAGCATTAACAAACCAAAAATGGAATTATTATTTGCTGGAAATAGCATTAGTAGTGAAAGCTTAGTAGCAGCATTAAAATATGTTGAAAACAAAAAGTTTGCAATTAATGTTATTTCAAAAAGTGGAACAACTTTAGAACCTTCTATTGCTTTTAGAGAATTTAGAAAATTATTAGAAGCAAAAGTTGGAGCAGTAGAAGCTAAGAATTTAATTGTGGCTACTACTGATGCTAAAAAAGGCGTTTTATACTCATTAGCAACTCAAAAAGGATATGAAAAATTAATTATTCCTGATGATGTTGGTGGACGTTTTAGTGTTTTAAGTCCAGTTGGTTTATTCCCATTCATTTGTGCGGGAATTGATATTGAACAAATTCTTAAAGGTGCTCAAGAAGCTAATAAAGATGCAAATGATTTAAGTTTAAAAACAAATGATGCTTATCGTTATGCGGTTGCAAGACATGTTTTAAGTAAAAATTACACCATGGAAATGATGATTTCTTATGAGCCAAAAATGCAATATTTTGCAGAATGATGAAAACAATTATTTGCTGAAAGTGAAGGGAAAGATGGCAAAGGATTATTCCCAACAAGCGGAATTTTTTCAACAGACTTGCACTCACTTGGTCAAATGATTCAAGATGGAAATAAAGTATTATTTGAAACAATTTTAGTTTTAGATAAACCAAAATACGATATTACTTTTAAAGCTGATAATGAAGATTTAGATAAACTTGTTTATTTAGATAAAAACAAATTGCATAATGTTAACATGACAGCTTTTACAGCAACTTTAGATGCTCACATGAACACAGGTAAAGTTCCAAACATTGTAATGAATTTTGCAAAATTTGATGAATACACATTAGGTTACTTATTCCAATTCTTTGAAAGAGCTTTAGCTATTAGTGCTTATCTTTTAGGTGTTAATCCATTCAACCAACCAGGTGTTGAAGTTTATAAAGCTAATATGTTTAAAATTCTTAAATAATAAAAATTTAAATTATAAGTCTTGTTAAAAGGCTTATTTTTATTCTAAATTTTATTCAAAAAGCCTAAAATGGTGGAACACATTCCACTGAATTTGGTGGAATGTGTTCCACCATTTTAGGAATTTTTAATAGACTCTAATAACTTTTAATATATAATATTAATAAATAAAAAGAAATATTTATGACATATAAAAAAGAAATGTATTATGAAAGATTATTAGATCCAATAATAAGAAAATATTTAAAAGTTTTTGGAGCAATTTGCATTCAAGGACCTAAATGATGTGGCAAAACATGAACATCACAATTTCACTCAAGCAGTTCATTTTATGTTGGAGATTCTAGAAATAATTTTGAAAATAGACATTTAGCAAAAATAAATCCAACTAAAATTTTAGAAGGAAAAACACCAAGACTAATTGACGAATGACAAGAATATCCACCTATTTGAGATGTAGTTAAATTCGAAGTAGATAACCGTGGACTTAAAGGTCAATTCATTTTGACTGGTTCTTCTACTCCTGAACATAAAGGCCTATTTCATAGTGGTATTGGTCGAATTGCTACATTACAATTGAGACCTATGAGCTTATTTGAATCTAAAGATTCTTTAGGACAAATTTCATTAAAAGATTTAATAAATGACAATTTTGAAAATACAACTTATGAAGGTATTGAATTAAATAAAATTATTAATTTAATTATTAGAGGTGGTTGACCTGACAATATTGAAGTTGATGATAAATACTCTTATTTAGCGGCAAGTAATTATGTAAACACTTTTTTAACTGAAGATTTAGATAAAATTAGTGATACTAAATTTGATAAAAATAAATTACAAAAATTATTAAAATCATTAGCTCGAAATAATGCGATAACAGTTAGCATTAATAAACTAATTGATGATATTAAAACAAATGAAAATGAAACAATTGATAAAAAAACTATTTATGCATATTTAGATATTTTAAATAGATCTTTTATTGTTGATAATTTGCAACCATTTAGTTTGAATATTAGATCCAATTTAAGAATTAAACAAAATGAAAAAAGACTTTTTGTCGATCCTTCTTTAGCAGCTGCGTTATTGAATGTTAATAATGAGAGTTTGCAAAATGATTTAAATACTTTGGGTTTTTTATTTGAAAACCTTGTCTTAAGAGATTTGAAAGTTTATGCTCAAAGTAATGAGTGAAATATTTATCATTATCAAGATTATAATGATAATGAATTAGATGCTGTTATTGAATTAAAAAATGGCGAATGAGTTGCAATTGAAATTAAATTAGGCTGAGAACAAGTTGATAAAGCTTCTGAAAAATTAGTTAGATTTTCTAACAAATTAGAAAAACAAAAGGCAACAAAACCTAAAAAATTAATCGTAATTACAGGAACAGGGAAAAGTGCTTATTTAAGAGAAGATAATGTTTATGTTGTGCCTATTGGATTGTTAAAAAATTAAGAAAAAAGAATATTGCTAATTAAAGCAATATTGCTACATTGTAATCTGAAACTTTACACATTTTAGATAGAATCTATAATGGGTGAAGTTTTTTTTATAAAAAAAGTCACCCAAAGAGTATTTGGCGACTGTCATGAAGAAATGCAAAAAAATTATCATGAAATGAACGCACTGTTCTTGAATACTTACTTTGAACAACTAATTATAGTATAACAAAAATAGCAAAAATATTAGGTTATTCAAGAACAACTATTAATAATGAAATAAAAAATAATTCTACTTTTTGAGGTTATTTTGCTTTATATGCAATTGAAAAAACAATTACAAGAGAAAAGTGAAAAAATCATTTTAAATTTTTAAACAATTTAAATAAATATAAAGAATTTTCTTCTTTATTTAAAAAAGAATATGATGGTAAAATGCGGACTGTTAAATTAACAATTGAAAAAATAAAAAATCTTTATCCTGATATTGATGTTCCTTCTTTTAAAACAGTCTACAATTGAATAAAAAGTGGTCATTGAATTTTGAACTGAAAAGGTTTATTAAGAAAAGTCTACAAAAAAGGCGAAAAAAGAGAGAAACAAAGCGCTGCTAGAAGACTGGTCGGCGCACGTTATGTAAGACCTTTTTGATCTAGACCAGCAAACATAAATGAAAGAAAAGAATTTGGCCATTGGGAAATAGACCTTATTGTAGGTAAGAGCAAAGGCACACATTGTCATTTACTTAGTTTTACCGAAAGAGTCTTTAGATATGGATTTTTGGTAAAAATTCCTAATAAAAATCCACGAAATTTAAATTTGTATTTATGGAATCTTATTTGAAAACATAAATTAAATGTTAAATCTATAACTCAGGATAATGGATTAGAATTTTTAACATTATTTTTCATTGGATATAAACTAAAAATTTTTATATATAAAGCTGATCCATATGCTTCTTTTCAAAGAAGTTCAAATGAAAATTTCAATGATTTAGTTAGAAGATTTTTTTAAGAAAAAGACTAATTTTGATGATATTACTGAAGAAGAAATTTTAAAAGTACAAAATGAAATAAACAATCGTCCAAGAGAAATTTTTGGATATTTATCTGTTTTGGAAATGTACGAAAAATTGAATGCAGAAAATCCTTGAAATTCAACAGGTATTGACAAAGTATTATATGGAAAACAAAAGAGAAACTGAGAAAGCAACAAGAATAGAAATTTGTTTTTCAAAAAAATAGGAAAATTAAAATAATATTTTAGAAAGGGCGACAAGTCGCCAAATATTTTAGGAAGGGTTAATGCCCTCACTTTTATTTGTCCTATTGGACAAAAAATAATAACAATATTAATAATTAAAAAGGAATATTGACATGCAATATTCCAAATTTCTAGGTGTAAACTTACAGATTACAATCTAGGAATATTGCTAATTAAAGCAATATTCTATTTTTAATTAACAACATTTGCATTCTTTGCGGCATTTTTCTTTGCAACATGATGAACAACATGAATTTTTGCAGCAATCTTTTGTTGTACATTTAGTTGCACAGTCTTTTACTTCACAATTTTTTGTGCAACAATTTTTCTTTGAACAAGCCATAATTTAACCTCCAATGCATATATATTAAATTATATTCTTTTTTTAATATATAATTAAAACATTATGCCAGAATTACCAGAAGTTAGAAGTGTGGTCAAAGATTTAAGACCTAAAGTTGTTAATAGAAAAATAGTTAAAATTGATATCTTACATCCTAAATTAATTAAAGAGGTAAGTGTTGAAGAATTCAAGAATTTTTTAATTAATGAAACATTTTTAGATGTCAATAATTTAGCTAAACATATTATTTTTTCTTTAACAAATAATAAATATTTATTAAGCCACTTGAGAATGAGTGGAAAGTATTTTACACATTATAAATATCGTCCAGCAACAAAACATGATTACTTAATTTTTCATCTTGATGATAATAGTTGTATTTATTATAATGACTCACGTCAATTTGGCACTTTTCATATAAAAACTAAGGGCACTTTATATACAACTAAACCTTTAGATAAAGTTGCAAAAATTCCTTCAGAAACAAATATTAAAGAGTTATTTAATAAAATTAAAAATAAGAATATACCTATAAAACAGCTTCTTTTGGATCAAAGCTTTGTTTCAGGTATTGGAAATATTTATGCTAATGAAACTTTATTTGCCACTCAAATTAATCCTTTGACGCCTTCAAAAAATATAACTTTTGAACAGTTAGAAAAAATTATTAAAGCAGCTGCAAAAATTATGGATCAAGCTACTGAATTAGGTGGTTCAAGCATTGACACATATACTTCAGTTGATGGAGTTAAAGGTCAATTTCAAGATTTTTTACAAGTTCATGGACACTTTAATGATGTATGTAAAAGATGTAAAAAAGCTAAAATTAATAAAATTTTTATTAACAAAAGAGGGACTTATTATTGTCCAAATTATCAAAAATAAAAAGCACTTCTCAATAAATGAGAAGTTTTATTTTAATCTTTTAGTAGACTAATTGGAATAATAAGTATTCCATCTGTTATTTCATATGCTCTATCTTCTGCTGTTAAAATAATCATAAGATCCGGTTCTCTTTGTCCATTTTCTTTTATCAGCTGTTTTACTTTTAATAAATTATTTTTCGCTTTTTCAATTTGATTTCCACCTAATTTACATTCTATCAATGCATATCTGCCATCATTTAAGTGCAAAACAAAATCAATTTCAAGACCATATCTATCGTGATAATATGATAATTCTCCATTTAAACTTGATGAATATATTTTTAAATCTCTAGAACACAAATTTTCAAATACAAAACCATATGTTCGTAAATCCTTTATTAAATCTATATATGAAATACCTAAAGAACTTGTTGCTAAAGAAGGATCTACAAAAAGTCTCTTGTTTGTACTTCTAATTGCTGAAGCTGACCTAATTTTAGGGTTTCATGCAGTTATATTATCAATAACATTTAAATCTTCAAGTTTTGTAGTGTAATCATAAAAAGTTGTTTTTGAACCTTCATCTTTAAAACTACAATCTTTTATTAAAGTTTTTGAAGTAGCTAAAGTAGCAACATTTCTTGCATAACTTTTCAATATTCTTCTAGTAATTTCAGGGCTTCTTTTTATGTTATCAATTTTACTTATATCTACATTGCAAATTTGATTGACATAATCTTTAGGAATTTCGATTTGATTTTCATATTTTTTTAAATTTAGAGTACTTGGCCATCCTCCTCTGCAAATTGCTTTTGCTAAATCTTCAAAAGATAAATTTGATTTTAAACCATTTACTTTAATTTTTTTATCAAATAAATCTTGTAAAGATATTTTTCCACTAGATTCTCCAGTTTCATATAAACTCATTGGATACATTTTCATTGTATGTATTCTACCAGTACCAGTATGCATAGTATTTAATTTATTGTGTGTACTTGAGCCTGTTAAAATAAATAACCCCTTTTTTCCCATTGCTGAATCAACATAAGTTCTTACGGCATCTCACATAATTGGAAAGTCTTGTCATTCATCTATTAATAATGGTTTTTCTCCTTCTAAAAGTAATGAAGGTTTATATTCAATTGTTTCTTTGTAAGTTTTTAAATTGTCTGGATCTTGTAATTTAATTAATGTTTTTGAAAATTTTTGTGCAGTAGTTGTTTTACCACATTGTTTAATTCCTTCTATGTTTATAGCACCAAAAATTTCAAGATTTTTTTTTATTTGTTTTTCTATTATTCTTCTTTTATATTTCATAGCAACCTCGTAAACATTATAACAAAAAAATAATTTGCCGAACACTTTTGTGGTAATTTGCTGAACACTTTTGTGGTAATTTGCTGAACACTTTTGTGGTAATTTGCCGAACACTTTTGTGATAAATAATATAAAAAGAACAAAAAAATGAGCATTTAATTACTCGCTTTTTTGTTCTTGAAGATTATTTTCTGGTTCTTTTTCTAAAACTCTAATAACTTCATGAGAGTCATTAAAGTTGCAATTGCTCCATAAATCCTCTACATATTTTGATTGATAATTTTCTAAATCAAATTCTTCTAAAACATATCATTTTTCAATAGCTTTTATCAAATAATAATTAATTATTGGGGCTACTATTACTAATACTCAAAATCCTAAATATAAGCCTAATTCAACTCATTTACTAATAGAGATGTCTTTACCATAGAAAACACTGCCTAGTGCTTTATCATTATTTACTATTCCGTCAATTGTTGCGCCTATATAAGCAACAATTGCCAATAATGATCCTACAATTGCAAAAATTGAAAAGATTCAGAATATAACTGTATTCATCTTTTTAATATTCAATGTTTTTTTGTGTAAATACAAAACAATAATAACAGCATAAAAATTGAAGAAGATAAATGTTGGCAAATTGCTTTAACCATCTAATATGTTATCGCCACCTAAAACTCAGATGCAAATTGAAATAATTAGTCAAATAGCACTATAAATAAGACTTATAATTAAAATCATCATTCTTTTAAGATTCATTTTGTTTCTTATTTTAGTAGTATTAATTATTATTTTTAAATTAATAGCATTATTAATATTATGTGCATATGAATTGGTAAAATCATTTAATACTCCAACAACAGCAATAAATAAGAATAGACTAAAAATAATGCTTAAACTTTGCACTAAATGTTGATTTGTTCTTTGATCAATTGTATTTTTAATTATTTGTTCAACAACTCCACTATTATGTAAAATTGCAAAAATAGCAATTAAAGAATAATTTATTATAACTGTAATCATTCCAACAATAGCAACAGTTGGTAAGTTATCTTTCTTTAAGTTATTAGCAGTAGATCCAACTGATAAAAATGTATAGTTAAAGCTGATGAAATAACAAAATTTTTTATTACCTAATTGTGTAGCTCAGTTGCTGATACCATTTGTTTTAGTATTTTTAAGTTGGTCAGTTTTGTTAAAACTTAAAGCATTATAAAGTGAAATTAAATTAACCCCGCTAAAATTCAAGTCATTAATCAACTTAAATTATCTTCACTAGTAGCTTTTTTATTAATTAATTTTTAAAAAATATCCCAAACCAACAACAGTTCCTATTAGCATAGTAAGTATAAAAAAACCAATTTTTTTAGCATTTTTATATGTGAACATTGCTTTAGCTCTTTTCTATGATTAATCAAAAATATTTTTTAGCAATAATAAAAATTATTTGTTTTTATTGTAAAATAAATTCATGAATATTTTTTATAGTATTTTGTTAAATTTAACTTTAATCTTAATTGGATATTTTTTACTTGGATCATTTAATACAAGCATTATATTATCCAAATGAAAGAAAAAAGATGATATTAGACTGCATAATTCAAAAAATGCGGGAGCGACTAATAGCTTAAGGACTTATGGTAAAAAGTTTGCAGCAACAGTTTTTGTTATTGATGTTTTAAAAACTTTTATTCCAACAATAGTTTTAGCAGCAATTTTAAATCATGCTTGTTTTGACTTTGCTCAAACTTATTACATGAGCCCACAATCTTTAGCTTTAGGTGTAATCATTGGACATATTTTTCCAGTTTACTTTAAATTTAAAGGTGGTAAAGGTGTAGCTTGTACAATTGGTTTAATTGCCACAATTAATATTGTCTTTTTATTAATGGCTTCTATTATCTTCTTTATAGTTGTCTTAACTTCTAAATATGTATCTTTAGCTTCAATATTAACAGCGGCAATATTAGTGCCCTTTGCTTTTATTCCTTGAATGACTAGTGGCATTTTAGGATATTGAACAAACTTTGTGTATGAATATCACAATTGCTTTGTAACAAGTTTTATCTATTTAATAGCTGCAATATTAATTATTGCAATGCATCATAGTAATATTACTCGTTTAATTAATCATACAGAAAGTAAATTAGGCCAAAAAAAAAGCGAAGATAATTCAGATGATACTGAAGTTGTTTTAATTAGTGAAAAAGAAGAACATGAATCTACTAAATAAGTAGATTTTTATTTTTTTGTGTTATACTAAATATGAAATTGACAGAGGTATTTGCTTATATAACAAGCAGATTGAGAAAAACTCTTAGAGCTGATCAGGTTAATACCTGCGGAGCAAGTTCAATTAATTTGAATTATAAATAGTTTTTTCTCTAATCCTTTTACTCTGTCGTGAAAGGATTTTTTTATGAAAAAAATATATAAATTTATTGTAGCAAGTTCAAGTATTTTTGCTTTAGGAAGTATTAGTGCTGGTTGCCAATATCAACCTAATATTGAAGAAATAAAAATTAAAATGGAAGGTATTAAATATGCAGATAAAGAAAAGTTAGATGCATATACAAAAGTATTTAATCAAGTTTTTAATATGAAAATGAAAGAAGCAAATTTAAATCAAAAAGTTGTTTCGTTTCATGATTCAGGAAATGGTGAAAGCGCAACACTTAAAGAAGTTAATTTAAACAAATTTCAAGTGGCTTTTCCCGGAGCTTGAAGATATTATCAAGAATATTTGAATAATAAAAATAAAAATGTTGTAGCTTTTATGCAAACAAAAACTAGAGCTTTTAAAACAAAAAGTAGTTTAAATTACAATGATCAAAGTCTTTATGATCCAAGTAAAAGTAATAACTTCGCAACTTTACAAGCTCAATATGAAAATGAATTATTCAATAGTGAAGGTGGCTATGTAAATTGAAGTGACAAAAGCATGGGCGGCTTTGTTGATTATAAATTTTTAAATATGTATGAAGCTGATAAATATGTAGATCATCAAAGAGGATGTATTTGAATTTATGGTACTAATGAAGAAAGAAATTTAATCAAAAAAGCTTGAAATGATAAAGATTGAGAAACTTTTTATAAAAAAGGAATTTATTATGGAAGTAATGATAGTGCATCAAAATATCATTTACCTCAAGCATTATTAAAAAAACAATTTAATTTAGACAAAAACTTTACACTAGATTCTTTTGCAAGTTCAAATTCCAAATATTTTAAAAAAGGAAAAGCTAAAGAATTGGGCAAGGATAATTCTTCAAATTATCATATAGCTTTTGATAATGAGAATTCTTTTGCTTATAGTAATCATGGAAATAAAGCATTAGAAAATAGAGATTTTTATGTTAAAGATAAAAATAAAACTTATGAAATGTTAACTTTAACTGATCCTATTCCTTATAATGTGGCTATAGCAAATAATTCACTAAGTAAAAATGTATTAGAATTATTAGCTCAAACTTATATTGAATTAGCAAATAAAAATAATAATTATGTTGGAAATTTATGAGGTCTAAATCATTATTCAGTTATTAGTAATGAACAAACAGAATTTATAAATAGAATAGAAGCAGTTATGAAACAATAATATGAAAACATACAAAATTGAATTTAAAAATGTAACTTTAGCTTATCAAAATGAAGTGGTCTTAGCATTTAAATATCATTTTTGAAGTGGCAAAACTTATGGAATTTATGGGCTTAGTGGGGTTGGGAAAAGTACTTTGATTAAAGCTATTTTAGAACCAAAATTAATAGCTAAAGGGCAAATATTAATTAATGATCAAGATATCAATAAAATTGATCAAAAGACCTATAAAACAGTTAAAAAAGATATTTCTTTTTTATTTCAAGAGCCTTCTTTAATTGAGACAGATGATGTCATTGAAAATATTAAAAGAAAGTCAAATAATATCTATAATAATTGATTTTACAAAGTTTTTAGAATATTAACTAAAGAACAAAAAAATAAGCTAGCAAAAATCTTAGAATATTTAGATATTTTTGATTATATTAATACACCAGTTAAAGATCTTAGCGGTGGTCAAAAACAAAGAGTGGAACTAGCCTCAATCTTTTTTGAAAATAAAAATATTATTTTGGCTGATGAACCAACAAGTAATTTAGATTTTATTAATACTAAAAAAATATTAAAATATTTGCAAAAATATGTTCATGATAATAATGCATTATTATTATGTAACATTCATGATGTTACTAATTCGCTAGAATATATGGATTATATTTTATTATTAAAAAATAAGAGACTTGTTTTTATTAAACAAGCTAATAATATAAGCGAAAAAGAATTTCAAGAACAATATTAAAGTCTAACTAGTTTAGGCTTTTTATTTTGAATTTCAATCAAATAGTTATGCTTTTGTGATATTTAATTAAGGTTGTAATTAATTCGATGGAAATTAAACAAAGTCAAAAGATTAATATTAAAGCTCCAACTTTTTGTCATTCATTTTTGTTTTGGTAATAATTTAATCTAGCGCCTAAACCAACAGCTGCAAAAGTTCCATAATTAATAATGTTTCTAAAGATTAATTCGAATCTAAAAGCTGCATAACTATAAAAATCTTTAATTACTCTAGGTAAAATAAATTCAAAATATAGAGTTATTTTATTTTTATTTAATAATAAATAATTGCCAATTAATTTTTGATCAATATTATTAATTGATTCAGTATAAAATTTACTCATTGAACGCATTGTAGTAAAAGATAAAGCTAAAATTAAAATGACTTCTCTTGAATAATAAAGATTAATTGATAAATAGAAAAATAAGGTTATTGGTATAGTTCTAAAGAATGCTAAAAGCATTTTACTTATTATTTTCACAAATTTATTATTTGTTTTATCGTTTGCAAAAAAAGAAGCAAAAATTGAAAAGATAAAAGCTAAGAAAATGCTGGCAATAGTAATTTTGATAGTAATTAGTAATTCTTTGAAAATAGAATAACTTTCGCTTTTCAAAATTGAATAATTTGCTTTATAAGTTAATTCACTTCATCTACTTTTAAAAATATCTAAATTAAATTGATATTTAATATTAATAAGTGTAATAACACTCGTTAAAAAAATTAATGAAAAACTAATTATTAAATATAGTTTAACTCGAACTAAACCTTTTATTTTATTGTTAATTTCTAATTTAAGTGGATTAAAGATTAAGTGATTAATTATTAAACTATAAATTTCTAATATTAATAGTATTAGTGCTAAATAAAGAAGTGGAATTGCAATAACGCTATAGTTACCACTTTTAAGAGGATTATTGATTAATTCTCCAATTCCAAAAAGTCCTAAAGTACTTAAAACACTGCTTCATCTAATATTTGATTCAAATGAATAAAGAAATAACACTATTACTTTATTCTTATTACGATAAGAAACATTATTAATAAAAGAAGCAAATTTATTTTGTCCTAGTAAAATTGAATTTCAATAAAAATTAACTTTACTGTTTTCTCATAATTCACATAAATATTTATGCAATCAAAGTCAAGTAAATCAAGTCAATATCAAAGAAGCTGAAAGCAATCTATCAAAGCTTGAAGTAAAAAGAAATACAAAAAAGAATATTGGTAAAGTTCTTAATAGCGTAATTATTATTTTACTTATTATTGGTAATATTTTATTTTTATGTAAATTTCAAGAAGCTAAATAGCTTGTTACTAAAGCTAAAAGAAAACCAAGTAGACTTCCTAATGCAGTATATTGAATGGTACTTCATAAAAAACTTAAAGATAAAATTCATAAATTATTTGCGGGATATGAAGAGTTTATATTATTAAAAGTAAATAATCTTTGTAGATCATTTTTTAATTGTTTTACTGAATTATTGTCCATAATTTTTCAATCAAAAGATAATAATGAATATAAACAAATTATTAGAATAAGCAAAATAAAAGTTACTTTAACTCATCTTTTGAGTTTTTTATAACTTTCATTTTTGCTATTAACATAAATATCAAATAATCATGAATAATGATATTTTTTGTTTTCTATTTGATTTGTTTGCAAAATAAAATCCTTTCATGATAGAGTAAAAGGATTAAAACACTTGAATTCAAAATTAATTGAACTTGCTCCGCAGGTATTAACCTGATCAGCTCTAAGAGTTTTTCTCAATCCACCTGTTTTATAGGTAAATACCTCTGTCAATATATTTATATTTTACCATTAAATTAATAAATAAGCTGTGCTTTTAGAAAATAATTTTTACCTTTTTTGGTGCTTTTTATGGTTATTTTTAAGGCATAATTTTCCTTATGTCCCAAAAATTTAATTTTTTTATGTTAAATATAAGGAGACAACTATGAAAAATAGCACAAAAAAAATTGTAGCTTTAACAATGGATATTTCTGCCGGGATTGTTGGTGCAGTATCAGCTGGTACTTTAATTGGTTATGCAGTTGACCATTCAAACTATAAAAAAGAAAAATTGTGAATTGAAAACAATATAAAATACCTTAAAGAATCAAATCAAAAATTAAAAGAAAAAGACAACAAATTAAAAGAAATTTGAAAGAAATATGAGAATCAAGAATGACTTTCTAGTCTTTGAAATGAACTAAGTTTGTGTCAAACTCAAATTGACTCAAATGAAAAACTTATTAAAGATTTACAAGGAATTATTGATCAACACTCTTCATATCATGTTGGCCGCCGCTTTGGTAACAAAAGTTATATGAGAGCTGTAAAACATAGTAATGAAAAGAAAATAATTCAAAATCTTTTACCAGAAATGGTGGAAAGAACAAAACAAGTTATTAATCAAAGCGAATATGAAAATATCAAAAAATCAATCCAAGATTTTGTTGTTCGTAGTAATGATTTATTAACTTTAAAAACTGCAGATGAAAAATTTGTTGATGCTAATAGTAAAAGTGAATTAAATTCTTTAGAACTTAAAATTAAAGATTATCAAAACACTTTAAGTAAAATCGAAGAAGAAGCACTTTCAAGTAACACAAAACCTAACATTTCAACTGAAATGATTAATAAGGCAGCAGATTTTATTAAATCTGATTTTCATGTTTGAAATGACAAAATTATTAAAAAATATGTTAAAAAGACGGCTTAATTAAAAATTCATGTCAAACATAGATAAAATAGAAGAATTATCAGCAAAAATAGAAGATGCTAATACTATTGCAAGTAATAATTCTCCAATTTTAAATCCTATTAATGCTGTTAAAAAGATTTTATTGTCAAGATTTGACTCTAATAATTATGACGTTATATTAGAAAAAAACGGTGTTTCAGTAAATAGAAGTGTTATTATAGAAGATATTATTAAAGCATGTAAAACATTAGAACAATCATATGGAAATACGAAGGAATATATAAATCGTTATGTTAATACTTTTGTCAAAACAATTGAAAATACAAAATTATTTAACAAATCTCAAATTCTAGACTTAAGCAACAAATCATTAACACCTATTATTAATGGTCTTAATGAATTTAACTCATTTGTTGAAAGTTCAAATCAAATTTCTTTTGAAAAATTAGATGAATTTATTAACAAAATTCAAGCTATTCTAAATGTTGAAAATGATTTAACAACTTCTATTCAGTCTGTTATTAAAGAATTTTTAAATGCCTATAATTCAAATGCTGATTCAATTGAAACAACAGTTAAATTAGCTAAACTAAATAACTCTAAACAAATTATTGATGAATTTGTACAAAAATCTAAAATTGAATTAAGTTCTCTCAAATGAGATAATTATATTGAAGGCAGAAACAAATTAATTAATGAATATGTTAATAGCAAAAATGAATTAGATTCAATAAAAAATGGGTTTAAAAAAGCTTTTGTTAATATTAAATCAAGATTAAATGAATTTAATTTAACATTAGAAGCAAACAAAACAACATTAAATGAATTTTATTTATTTGAAACTTCTTATAAATCATAATTGATATACAAACTAAACTAGATCCTTTATTAAAAGCTAATTTAGATTCTGATATTGACAACAAAATTGATAATATTTCAACATTCTTTGAATAATTTCAAATCTTAAAGAATATGAAACAAATTATTTAAAAATATTTCAAATTATAAAAAATGCAACTGATTTAAACTTACAAAAAGTTAATAACTTTGCTTCAAAACCTAAAGAAATTTCAAAACTTATTAGTAATGCAAATCCAAATATTGCTAATTTCTTATCAAAAGTTTTTGATAATTTAAAAACATTAGACCAATTCGAAGATGTTAAAAAAATAATTGAAGATATTAATAATAACAAACCTAATTTATCAACTAAAGAAATATCTTCAATTGAAAATAGTGAAATTCAAAATGACAAAGAAAAATTTAAAGGACTATTTGAAAAAATTAATTTAATCAATAATTTTATTTCATCAATTAATGAAAAAGATAAACTTTATAGTAATTTCTTAGAAAATGAAGCTCAATTTTGCTTAGAATCATACAATGAAATGGATGCAAAATTAGCAGCAATAGCAAATGTGGATTCTAAAGTATATGAATATAAAAACAAAATTTTTGATGCTGCAATTGAAGCTTATAAAAATGTTAATTCTACAGAAAACTTTAGTGCTGTTCAAGCTGATAAAGACAACAAAGTTAACTTTGTTAAAAAATGATTTGGTGTTCTTAATTACTATAATGAATATGTAAAAGAAAAAGCAAAAACTGAACAAAAAGCAAAATTAACTTCAGAACAAGAAAGTTCACTTGTTTTTGCAAAAAATGAAATGAATGCAATTAAAAATGATCAAATTTCTAAATTAGATAGTGAAGGTTTAAAAGCTTTGCAACAACTTGATGGTGGTGTTGATTTTACAAATAATGATTTAATTAAAAATTATTTAGAAAATGATTATTTATCAGCTGTTAAAGATTTAAGAGAATATTTCTTAAACAAACTAATTTACTTATTACATGAAAAATTTAATTATGGTGATGTATACTGATCTCCAACAAAATTGTATAGACCAACATCTGTCTCACAAAATAATGCAAATTGAGATGAAAAATCAAAAATTTGAGCTAAACCTAAAGTTTTAGGCAAAAAACATCTATATGATCGTATTTCAGATTTAGATACTGGAGGATACTCAAAAGGTAATACAAATGGAGGCTGAGGAAATTCTAAAGGTGATAAAGTTAGAGCTGAATATTCATGACACCGATATGGAGTAGATAAAGGAAATACTACTAGTGAAATCAAACAAGGTGAAGAAATTGAAAATGATAATTATAAACCTTGACATACAATTATTAACCGTAGTCTTACTGGTAACCAACCAGCAGTTAAATATATAAGTCAAAAATATCCTAATATGAAATCAAATGGTTTTTTACAAGCAGTAGGTGATGATATTTGAAGTGGGCATGATGATCTATATACTTTGGCCAAATTAATAGAATATGAGAAAACAGTAACTGAAAAAATGCTTTCAAATATCAAAAAAATATCAGATTCTAAAATAATGTTTAAAAATGATTTTAAAGACAATTTTGTTAATTTAAACGATGCTCTTTACAAAATAAAATATGCTTTTGATTTCATTAAATATAACAATCCAACAAGCAATGGATATTATGAAGGAATATGAGAAAGATGAAGTGGAGAAGAAAACAAATCTCATTACGCAATTGGAAATAATCCATTAAGAAATAGAGTTTGAGTTCATAACAACTATGGAGAATCATATACTCTTCCGGGTCCAGATGGAAATCCAACAGAGAGTGATTTTAATGGTCCTTACTATAGAGCAGCTGCTCATACATCAGATGAAAAATATGATAGTACTAACCCACTATTTGAATATATGCCATTTTCTAATACATTATTCTCATATTTAAGAACAGTAGACCATGCTTCAAATTCTTTCTTATTAAAACATAATGGTGAACCTATTTCATGAGTAAATTACATCTTTAAATTTACTCTTGATAACCCACTAGGAAACTTTGATGCTTCATATTACTTATGAAATAGAATGTACTGAAAATCAGAATATGCAATCACCGAAAGATACATTTTGAAAAATAATTCGTCTTCTGAAACAGTTTCAATTTGAGATAAATGATCAAATATCTTTACTACAACAAGTAATAATACATATAGCAAACTTATTTGATAAATTAATGAAATTTAATATTAAATGAACCCCAAACTTTCTTTTTCATAAGGGGTTCATTTAATATTTGAATTACAAAAAGAAAGATTGTTTCCAATTTTGTGTAAAGTCTCTCCCTTTCTCAATAGAAAGGAATTAACTTTAGATCAACACCTAACAATATTGTAGAACAAATCAGATAATGTCTAAAAAAAACATTATCTTATTTTTAAAATTTGAATTTTAAAATAGGAAAAAAGATCTTGCTATGCAAGAAATCTTAATTCCTCTCCCCTCTCCCTTAATTCATTTAAGGGAGAGGCCTTCTTTGAAGCCAAAGAAGCAAAGCTTTTAAAATTTGAAAAAATTATTCTTCATTTTCAAAATCAATTATTTTAAATTCATTTAAAATGCTAGATCAATTTCTAATTCTTGAATTACTTCATTTTTTTATGATTTGTTGAGATGCTAGAAAAATAATTTTTAAAAGACTATCATCAGATGGGAAAACCCTTTTTGTTTTTGTATATTTTCTAAATACACTATTTAAATTTTCGATTAGATTAGTAGTATAAATAAGCGTTCTTATTTCAGGAGAATAATCAAACATTGTAGAAATTTCAGATCAATTTCGTCTTCATATTGAAATAGATGTAGGATATTTTTTGCCTCATTTATCTTCAAAAATATCCAAATTATCCATTGCATTTTTTACATCATTAGATTGATATACAGATTTTAAATCTTTGCAAAATTCTTTCATATCTTTGTAGTTAACATATTTAACAGAATTTCTAATCATATGTACAATGCAACGTTGTTGCTTACTATCAGGAAAAACAGCTTCTATAGATTGTTGTATACCTTTTAAATTATCTGAACAAATAATATTAATTTTACTAATTCCTCTATTTTTAAGAGCATTTAGGACAGACATTCAAAACTTTGCAGATTCATTTTCTCCAATATAAAGTCCTAGAATTTCTTTTTGTCCATCACCATTAATACCCATAATAACATATGTTGCTTTCTTAGTTACAACATTATTATCTACAACATGAAAATGTACTGCATCAATATAAATTATTGGATAATATGTTTCAAGTTTTCTTTCTTGTCATAATCTTACATCTTCGACAATTTCATTAGTTAATTGGCTTATTTGTGCTGGAGAATAATTAACGCCATATTTGCTAAACATAAAATCACTTACATCTCTTGTTGGCATTCCTCTAGAATATAAAACAAATATCATATCAACTAATTCAAAAATATCACGTTCATACTTTTTGATGATTATTGGTTCAAATTTTGCTTCACGATCTCTTGGAATGTGACCATATTGACTATTAACATTTTTCTTAGAAAAACCATTTCTATAATTTTCTAAAATTTTTCTGTTATTTCATCAATAACAGGATTTATTGGTTCTCTTTTCATAAATAAAAAATCCTTTCTTGATAATATTTTATCTATTAAGAAAGGATTTTAAAACTTTACACAAAACTTTTTGTAGTCCAACTTAAAAATAATAATTACTTTTTGTATGGATTAGTCATAATATTTTTTTAATCCAAATATTAAATGAACCCCTTTTGAAAAGGAATTANGGTCATATATATTAATTTATAATTTATCAATAGTTGCTAATGTATTATACTGTGTAGTAGAATTGACATATCCAATGACTGTCAAGATTATTTCAATGACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCGTTGAGCACGAATTAAGGATCATGGAAGTCTAATTACATGTTAATCCCATAAACCGTGTAATGAGCTTCTCCTCTCCAGGAAAAATAATGTATACTTCATCCTAAACGAAACCGGTATCAGAGAGATTTAATTTAAGAAAGTAGTTATAGGTAGATGGAATATTTTCATATTAAGGTCAATAGATTTCATATGTAGTATGAAGCATTAAAGTTTCCTAGCGGGTTATCAAGAGTAAATTTAAAGATGTAATTTACTCATGAAATAGGAGCTCCATTATGTTTTAATAAGAAATGAACTGAATTATGGTCTACTGTTCTTAAATATGAAAATAATGGATTAGAAAATGGCATATATTCAAATAGTGGGTTAGTACTTTCATATTTTTCATCTGATTTTCCATTTTCTGTTCTATAGTAAGGACCATTGTAATCACTTTTTACTAGATTTCCATCTGGACCCGGAAGAGGACCGTATCAATTCCCTTTTTCTTCATCATAAACTATAACTCTATTTCTTAATGGATTATTTCCAATTGCGTAATGAGATTTGTTTTCTTCTCCACTTCATCTTTCTCATATTCCTTCATAATATCCATTGCTTGTTGGATTGTTGTATTTAATAAAATCAAAAGCATATTTTATTTTGTAAAGAGCATCATTTAAATTAACAAAATTGCCTTGAAAATCATTTTTAAACATTATTTTAGAATCTGATATTTTTTTGATATTTGAAAGCATTTTTTCAGTTACTGTTTTCTCATATTCTATTAATTTGGCCAAAGTATATAGATCATCGTGTCCACTTCAAATATCATCATTTACTGCTTTTATACAATCTGCTCAATCTTTATCAGGATATGTTTGTTTTATATATTTAACTGTTGGTTTACCATGATCACCAGGGTCAAAAATTGTATGTCAAGGCTTATAAGGTTCATTTTCAATTTGTTTACCTTGTTCAATTTCACTAGTACTATTTCCTTTATCTACTCCATATCGGTGTCATGAATATTCAGCTCTAACTTTATCACCTTTAGAATTTCCTCAGCCTCCATTTGTATTACCTTTTGAGTATCCTCCAGTATCTAAATCTGAAATACGATCATATAGATGTTTTTTGCCTAAACTTTAGGTTTAGCTCAAATTTTGATTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGATTTCTATCATATGTACAATGCAACGTTGTTGCTTACTATCAGGAAAAACGGCTTCTATAGCTTGTTGTATACCTTTTAAATTATCTGAACAAATAATATTAATTTTACTAATTCCTCTATTTTTAAGAGCATTTAGGACAGACATTCAAAACTTTGCAGATTCATTTTCTCCAATATAAAGTCCTAGAATTTCTTTTTGTCCTTCACCATTAATACCCATAATAACATATGTAGCTTTCTTAGTTACAACATTATTGTCTACAACATGAAAATGTACTGCATCAATATAAATTATTGGATAATATGTTTCAAGTTTTCTTTCTTATCATAATCTTGCGTCTTCAACAATTTCATTAGTTAATTGGCTTATTTGTGCTGGAGAATAATTAACGCCATATTTGCTAAACATAAAAATCACTTACATCTCTTGTTAACATTCCTCTAGAATATAAAGCAAATACCATATCAACTAATTCAGAAATATCACGTTCATACTTTTTGATGATTATAGGTTCAAATTTCGCTTCACGATCTCTTGGAATGTCAATTTCCATTTGACCATATTGACTATTAACATTTTTATTAGAAAAACCATTTCTATAATTTTTTTGAACTTTATTATCATTTTTTTCATAACCCATAAAATTATCAAATTCTTTGTTCATCATTTTTTGGATTGTATTTTGAAATACTTCTTTCATAGCCATACTAAGATCACCAGAAGTAACTGGATTATAATTTTCTAAAATTTTTTCTGTTATTTCATCAATAACAGGATTTATTGTTTTTCTTTTCATAAATAAAAAATCCTTTCTTGATAATTTATTGTCTATCAAGAAAAGATTTTAGAACTTTACACAAAATTTTTTACAGTCCCATTTTTTCTTTTAATGCTGTTTTATAGTGTATTTAGTATTTTTGCCTTGATTAATTTTAAGTATTTTTTTAGCTTTTAATAAGACATTTAGTTTTCTTTCAATTGTTCTTTTACTGCTTTCAGGTAGTAAATTAATTAGCTCATTTTTACTTACTTTTTGATATTGAGCTATCAGTTTTAAAATGTTGTCCGCTACATCAATTTTTTTATTTTCTAAACTTGTAAAACGGCTTTCAAATTCTTTATAAGCTTGTAAAACAATTTTTAAAATAAACTCTATAAAGTAATAATAGTTATTCAAATTATCATGTCATTTATATGAACTTTTTTGTTGAACATTTTGATATTTTTCCAAGTTTTCAAAAATGATTTTTTCTAAACTTATATACTGAACGACTTTAATATCACCAACACTTAATAGTCAATTTAAAAGAATTCTAGACATTCTGCTATTGCCGTGATTAAAAGGCGAAATGCAAATAAAATCCAAAACAAAAGCTGTTGTTAGAAGCAATGAATTAATCTTCTTACTTTTATATTCTTCATTATATTTATTACATAAATTATTTATATATTCACTAATAAAAAATGCTTTGACAGGAATAAATTTTATTTCTTGAGTACCTTCTAAGGTTGTTTCTAAAATAAAATTATCATTTAACTTAAAACGCCCACTCATTTTGGTATTTGAGTTTTGAAACAATTGATAATGAAGATCAAGAATCAAATTAGTGTTTAATAATTCAAATGTTTCTTGATTATATAGAGAATTTAATAATTCAAAATAACCTTTAATATCTTCTTCATTTTCATTTTGCAACTCATCATTTTCAAGCAAAATATCTTTAATTCTTTTTTTGCTTATATTGATGTTTTCTATTTTATTTGAATAAACTGCACTGTCAATTAAAGCTTGTTTTTTAAGCTTTTGGATTTCTAAATTCTTGTTATTGATATAAATGTTTTGTCTTCCTTTATATTCATATATTTCAGTCAACAAGTTAATAATATTTGTGGGGATACCTTTATTAAAAATTCTTTTATAATCAATATTTTTCATTTTTTTTGCTACCTTTTATAAGCCATTATATCATTTTTGGCGTTTAAAATGAAAAAAATAAATTTCACTTTTTGCTTCAAAATTAACAAAAAAGTTTTCAAAAAATCAAGAATAAGAAAAATAAATGAAAAAATTACCATAAAAGATACAAAAAGAATGCTATATAAATATATTTTTGGAAATATTTTCATATAAATAATAAAATTTTTATAGTCTTAATTATAAAAGACTTAATTTTGACTCAAGAAAGGAAGTATCTTTATGAAAACAAAGTACAAATATGTCGATGTTAATAAAATTATGGACGATCCAAATAAGATTATTAGACATTTAGACATAGATGGTAAAGTAATTGAAAAAAATTACAAAGCACCACTAAGTAATGCTGAAGTTTTAGAAGCTTACAAATGAATGGTACTATCTAGACAACAAGATACATACATGTTGCAATTGCAACGTCAAGGACGTATGCTTACATTTCCACCTAACTTAGGTGAAGAAGCATTACAAATTGCAACAGCAATGGCTATGGAAAATAAAGACTGATTTGTTCCAGCTTTTAGATCTAATGCTGCTATGCTTCGTTTAGGCGTTCCTATGATTGACTTAATTCGTTACTGAAATGGTCAAGAATGAGGATGCAACCATCCTAAAGATGTTAATGTAATGCCGGTTAATATTGTTATTGCAACTCAAATTTCACAATGTGCTGGTATTGCTTATGCACAAAAACAACTTAAAACTGGTGGTGTAGCTATTTCATTTATTGGAAATGGCGGAACTACTGAAGGAGAATTTAGCGAAGGTGTTAATATTGCAACTGTTCAAGAATGACCCGCAGTATTTTGTGTAAACAATAACCAATGAGCTATTTCAACACCTAACTCAGAAGAAAGTAGATCAGCTACTATTGCAGCTAAAGCTCATGCTTTTGGTTGTGCTGGTGTTAGAGTTGACGGTAATGACCTTCTAGCTTCTTATGAAGTAATTAAAGAAGCAATTGATTATGCTAGAAAAGAAAGCAAACCAGTTATTGTTGAATTCTTAACATGACGTCAAGGCCCTCACACAACTAGTGACAATCCAAAACTTTATCGTAGTGAAAAAGACGAAAAAGAAAATGAAAAATGAGAACCAATGCATCGCATCGAAAGATATATGAAAGACAAAAAAATCATTACTGAAAAAGAAAAAGAAAAGATTTGAGCCGATGCTCTTGAACAAGTTAAGAAAACTTATGAAGAAAGCTTGAAAGACATCAATACCAAATTAGAAGATATTTTTGACTATACATATGAAAAGCTTGATGATGATTTATTAGAGCAAAGAGAAGAAGCTATTAAATTCTGACAAGCTAAAGGAGGCAAATAATCATGGCTGAAGAAAAATTAACTTTAAATAATGTTCAAGCAGTTAATAATGCTTTAGACATTGCTATGGCTAAAGACCCCCGTGTAGTTTGTTATGGTGAAGATGCTGGTGTTGAAGGTGGAGTTTTCAGAGCTACAGAAGGCTTACAAAAAAAATATGGTAAAAGTAGAGTATTTGATACCCCTATTTCAGAAGCAACAATTGCTGGTACCGCAATTGGTGCTGCAGTAGCAGGTCTTAGACCTATTGCCGAAATTCAATTCCAAGGTTTTAGTTATCCAGCTATGCAACAATTATTTACTCATGCAGCTAGATGAAGAAACCGTTCAAGAGGACGTTTCACCGTGCCCATGATTTTAAGAATGCCTATGGGTGGTGGAATTAAAGCTATGGAACATCACTCAGAAGCTTTAGAAGCTATTTATGCTCACATTCCAGGTGTTAAAGTTGTTATGCCAGCCTTTCCTTATGATGTTAAAGGTTTATTATTAGCAGCTTTAAATGATCCAGATCCAGTAGTATTCTTAGAAAATAAGAAAATTTATCGTGCTGGGAAACAAGAAGTTCCAGCTGGCGAATATACAGTTGAAATTGGAAAAGCTAATGTCTTAACTCAAGGTAATGACTTAACTTTAGTAACTTATGGAGCTCAAGTATTTGACTCAATTAATGCTGTTAAAAAATATAAAGAAATCAATCCTAACGCTTCAATTGAATTAATTGATTTAAGAACAATTAAACCTCTTGATACTAAAACTATTGTTGAAAGTGTTAAGAAAACAGGACGTTTATTAGTAGTTCATGAAGCTGTTAAATCATTTTCAGTTTCTTCTGAAATTATGGCTAGAGTTAATGAAAAAGCATTTGAATATCTTAAAGCACCTATGACAAGATGTACAGGTTATGATGTAACTGTTCCTCTTGCAAAAGGCGAAGCATGAATGTGCATCAATGAAGACAAAATTCTTGCAAAAATAAAAGAAGTTATGGAATTTAAATTTTAATATTTAAAGACCATTTAATTTACTAAAAGACTTGTTTTATAGTCTTATAAGGAGAAATAATGAAAATTAAATCAACCCCTATTGCAAGAGCTATGGCTGCTAAACTTGGAATTGATATTAACTTAGTTCCAGGTTCAGGAATTGATGGCCGTATTCTTATAGAAGATATTCAAAAATTTAAAAACAGTCCTATTTCAGGTGCTGCTCATTCAAACTTTGCACCAGCTCCAGTAGCTGATAATAAAGTAATTACTCAACCTAGTGCAGTAGCTCCTAAAGCTACTCCAGCACCTCGTCCTGCCATTGATCGTTCAGCTCACTCAGAAGCTATTACTCCAATTAGAAAAGCAATTTCTAAAGCTATGACTAACTCATGAAGTAATGTTGCTTATACAAATTTAGTTCATGAAATTGATATGACTGCTCTTTGGGACTTACGTTCAAGAATCAAAGATATTGTACTTAAAAGTGATAATGTAAAAATTACATTCTTACCTTATATTATTAAAGCAGCTGCTATTGCTTTAAGAGATTTTCCAGTATTCACTGCTAAATATAATGAAGCAAATCAAACTTTAGATTACCCTGGTGTAATTAATGTTGGTATTGCTGTTGATACTGAAGCCGGTTTAATGGTTCCTGTTATTAATAATGCTGATAATTTAAGTATTGTTAATATTGCTAGTGAAGTAACTAGACTTGCAGGCGCTGCAAGAAATAGAACAATTAAACCTGCTGAAATGAAAGGTGCCGGTTTTACTATTACTAACTATGGTTCAGTTGGATCACTTTTTGGAGTTCCTGTTATTAACTATCCAGAATTAGCTATTGGTGGTGTTGGTGCTATTATTGACAAGCCTGTAGTTAAAAATGGACAAGTTGTACCTGGAAAAGTTATGTACCTAACTGTCGCTGCTGATCACCGTTGAATTGATGGTGCCGTAATTGGAAGATTCGCTTCAAGAATCAAAGAATTATTAGAAAAACCAGATGTTTTAGGAGTGTATTAATATGTACGTATTTAAATTTGCTGATATAGGTGAAGGATTACACGAAGGAACAGTTGGTGAAATCAGTGTTAAAGTTGGTGACAAGATTAAAGAAGGAGATACACTTTTTTCAGTAGAAACTGATAAAGTTGCTTCTGAAATTCCTTCTCCAGTAGATGGAATAATCAAAGAAATTAGAATGAAGACTGGTGATGTAATTCATGTAGGACAAGAAATATTTGTAATTGATGATGGCAAAGGTGGCTCAGTAGCTGCAGCTCCTGCTACTAAAGTTGAAGAATCGAAAAGTAGACCTGCTACTTCATCATCTACAAGCTTTTATGTTTTTAAATTTGCTGACATTGGTGAAGGTTTACACGAAGGAACAGTTGGTGAAATTCCTATTAAAATAGGTGATAAAGTAAAAGAAGGCGACACATTATTCTCAGTAGAAACTGATAAAGTTGCTTCTGAAATTCCTTCTCCAGTAGATGGAATAATCAAAGAAATTAAAATGAAAACAGGTGATGTAATTCATGTAGGACAAGAAGTTATTGTTATTGATGCAGGAAGTAAAAATCCTACTTCTTCTTCAGTTTCATCTGCACCAAGTTCCAATAAAGAAGAAGAAAAATGTTTAGTAGGTGATGCTCCAAATTCAAGTGAATTAATAAATCTAGATTTCAGTTCACTAAATTCACCCGCAAAAGAAACTCCTCAACCAGTTGCACCTCAAGCTTCAACTTATGAAGCTAACCAAGAAGAAGAAAGCAATGGTGTTTCATTTACTTCTGAAGGTAAAAAATACGAAGGCAAAGTTGATGAAGAATTTGATGTAATTATTGTAGGTAGTGGTCCTGGAGGATATTTAGCCGCTGAAGAATTAGGCAAAGCAGGCAAAAAAGTATTAATTGTTGAAAAAGAATTCTGAGGTGGTGTATGTCTTAATATATGATGTATTCCTACTAAAGCAATGCTTAAATCAACTGATGTTTTAGAAACCTTAACCGATGCTGCTGGTTATGGTATTGTTGGGAACTTAGATAAATTAAAGATAGATCTACAAAAAACATGAGTCAAAATGCATGAACGTAAAAAAGGCGTAGTAGATCAAATTTCAAGTAGTGTTAAAAAATTAATGATTGCTTCTAAATGTAAAATAGAAGAAGGCGAAGCAGAATTTGTTGGAGCTCACGAAATTAAAATAAATGGTAAAGTATATCGTGGCACAAATATTATTTTAGCCACAGGCAGTCACTCACGTAGATTGCGTGCTTTACCTGGATTTAAGGTTGGATATGAAAATAATTATGTTCTTTCAAGCCGCGAAGCAATTAACTATGATTCAAAATTGCCTGGATCGGTAGTTATTGTTGGTGGTGGTGTTGTTGGCGTTGAATTTGCTCAAGTATTTGCTTCAGCAGGTGCTAAAGTAACTATTATTCAAAACCAAAACCACTTACTTCCTGGTATTGATCATGATGTAACAAATGAAATTCTTAAACATCTAGAAAAGCATGGGGTTCAAATTATTTACAATGCAACTTCAACAGGTTTAAATAATAAAAAAGAACTTCTTTACGAAATTGGTGGTAAAGAAAGAAAAATTAAAGCTGATGTTTATTTGATTGCTGTTGGACGTATTCCATCAAGTAAAGGTATTGCAGAAGTTGGAGTTAATGTCGGAGTTAGAGAGGAAGTTCTAGTTGATGAAAAAATGAGAACAAATGTTAAAGGCGTTTATGCAATTGGAGATTTAACTGGACAAAACATGTTAGCTCACGTTGCATATCAACATGCTTTAATTGCTGTTGGTAACATTTTAGGTGAAAAAAATGTTCGTTATCACAATAAACCAGTTCCAGGATGTATTTATACAAACACTGAAATTGCTTTCATTGGTTTAACTGAAGAAGAAGCAAAAAATAAAGGATACAATATTTTTACTTCAAAATACATGTTCTCTTATTTAGGAAAAGCTATTGCAACCAAACAAACTTCTGGTTTTGTTAAATTAGTTGTTGATCGTGAATATGGGCAAATATTAGGAGCTCACATTATTGGAGCTCATGCAACTGACTATATTTCGGAAATAGCACTTGCTATGGAACAAGAAGTTAGTGTCAAAGAATTAGCTTACACAATTCACCCACACCCAACTTATTCGGAAATTATTTGAGAAGCTGCAAGAGCTGCTTCACTTAAACTTTACTTAGAAGAAAACAAGAAATAATTTTACTAATTTAATAATAAAAAAGGCACTTTGGTAAAAATCAATGTACCTTTTTTATTTATTTTTAGATTAATTTCTTAATTCTAAAACAAACAAAATATAGTATAATTAATATATTCAAACAAGAATAGATTAATCTAACAAAAGATATTTTTTTGAAAGGAAAAGAATATGCCTTACTCTCTCTCTCTCTCTCTCTCTCTGATCTATTTAAAATAGCAATTTTTAATTGCTCTCTTTTTGTTTTTTTATCAAATGATTAATACCTTCCCCAGGTATTTTTTATTATGCAAAAAATAAGAATATTTGAAACATTCTCTGGTATTGGTGCTCAACACAAAGCCATTACTTGATTAAATAAAAAACAAAAAGAAGTAAATTTTGAAATCGTTGCAAAATGCGATTGAGATATACAAGCAACCATTGCTTATGCAGCTATTCATCACAACCTTGATGAAACAAAAATAGAAAAAATTCTGAATTCAAATAAATTAGAAGATGAAACAAAAATTAATGAATATTTAAAGTCTAGAACTTTTAGTATGACTAGTAAAAAAGCTATTAGTAGCTTAAAAAACAAAAGTTATGAACTTAAAAAAGCCCTAGTAGCTGCAAATCTAATATCAAATAATTTTAATGATATTACAAAGATTACTAAAGAACAATTAGATCAGTTACATTTCGATTTAATAACTTATTCATTCCCTTGCCAAGGTCTTTCAAGTGCTAATATGGGTCGTTCGTTAGGAATTAAAAACAACAATTCAACTAGTCATTTAGTTTGACAAATAGCACGCGTTATTGAATTATTAGAGAACAAACCAAAATATTTGTTACTTGAAAATGTCCAACAATTAGTGACTAAATATAAAGAAGAATATAACGAATGAAAAAAGAAATTAAATAAACTTGGATATAGAACTTTTACAGCAGTATTAAACGGCAAAGATCATGGTTCACTTCAACATCGTAAAAGAGTTTTTGCACTAAGTGTTAGAAATGATTTAAAAGTTCCTTTTAAAGAAAATGATGAATCTTATTTAGATGAGTTACTTAATTTTGGCAAAGAAAGAGAGATTAAAAATAAATGAAAAGAATTTGCAAAAATTTTCGATTTAGAAAATAAAAAAGAAGAAGAATCAAAAGAAGCTTTAATCAAAAAAAGTCAAAGTCGCGAAAGAATGGCTTTTGAAAATAAAAATTATTCAACTATTTATAAGAATCAAAAGATTGATGAATGTCGTATTAACACATTGACAACCAAACAAGATCGTCATCCTAATGTTGGGGTTTTAGATTATGATGCTAATTTAAAAGGTTTTTATAACAAAAGATTTGTAACAAATAGAGAAGCTTACAAAATTATGGGATTTGAAGATAAAGATTTTGAAAAAGTTAATGAATTTAAGAAAAAAAACATAATAACAAAAGAAGCTCTTTATCGTCAAGCAGGCAATTCAATTTGTGTGCCAGCTATAAAAAGTGTATTTCAGTTAATAGCAAAAATCGAAGAATATAATGGAGGTCAAAATGATTAGTAGTAAACATATAGAATTTTTAAAAGAAAAAAAGTTTTTTAATTTTTGAAAAATTGGAACATCAGTTGAAAGCTATATTAAAAGAACAATTTTAAAATGAGATTGAGACATTATTTTTAATGCTTTTGCAGATATAAAAGATGTTAAATTTGAATTAGTTGATAGAAGTAACAATAAATTAAGTGTTTGTCTATATGAGTATGATAGAAAGTTTATTTTTCTTTATGATGAAGGCGAAAAAGAAAAAGAATTCTATAGCGCTTCAACAGTAAGACAAACAATGGAAGTTTTAGAAGAATTAAAAATATTAGATAATTTTCATGAAAATTATTACAAATTCGACGAAGATTTTATTATTGAATTAAAAGCTTATTTTGGAAGTAACTTAGAAAACGAATTAAACTATAAAAAAGTAGATTTGGAAAAATTTGTATATAAATATATTTTGCGAAATGTTAACAAAAAATTAAAACAAAATTTAATTAAAAAATTAAATAAATGAATTGAAGAATTTGAAGACAATAACGTAGAATATGAAGAAGTTGAATTAGGAAATTTTGATGAGAAATCAATAATCAACTCAATTATTTTTAGCTGACTTTCTTTTTCAAAAAACAAAAAAAGAAATAATCCTTTTTCAAAATGATCAGAATTAAATGAAAGAGATTTGTCTTATTATAAGGAATTAATAAACAAAATTGAATTAAATAATGAAAACACAAATTTAAATTCTTTTAATGAAAATATGGATGAATTATTAAACATTTTAAACGCTAAAAAAGATGATGATATAAGTAATATTTTATATACAAATGAAGAAGCAATTGTTACAAAAGATTTGGATAGAAATAAATTTTATATTGATGATAATGAAGCAATTTATAAAGAATTAGCTCCTTCTTTTGATGTATTAAATATTCAATCATATTTTAATAAACTTTTAAATATGAAGATATCTATACCAGTATTTCAAAGAAGTTACGTATGAAATGAAGAATACGTTTTAAGAATAGTTGGAGATTTAATTAATATTTCATCTAATAATTCCAATCAAAAAGAAATTTTAAATTTAGGAATTGTTTATTGATGGGAAAAGACAGCAAATAATCAGGTTAAAGAAATAATCGATGGACAACAAAGAACAGTGACATTAATATTAATTGCATATTCTTTATATAAATTGTTTTTACATGCTAAAAATTTGAAATTATTTTTACCAAAAGATCCTGTCGAAAATATGTTTAGATATTACATTGATATAAAAGACAATTCTAATAGTTTATTGCCTAGAAAAATAACAAGTATTTATAACAATATTAGTGAAAGTGATTCATACAATTCTCTTAGCATCGCTTTAGGTGATAATTGAAATGCAGCCGAAGGTAAAAATTCAGAATTTTATAAAACTAATATTTATAAAAATTTAAAAGCTATTACTAAGAAGTTATACGATTACTTTTTTACAAACATTAACGATGCAGAAGAAAAATTTAAAAATTTTGTTCAAGCATTTTATCAAAAAACATTTTTAACAACAATTACCACAAACCAAGAACAAACTCCTAAAAACTATGAATTATTTCAAAGAATTAACTTGTATTCAAAACCATTAACGTCATACGATTTAGTTCGTAATTTTGTTTATGGTCAACTAAGTGAAAAAAGTTCAAATGTAGAATCAAACATTAGCAAATTTGACAATTTCTTTAAACCATTATTTATTAGTAAAAGTGAAAGAAAAACACTTGTCTTGGATTCAAAATTGTTTGATAATTACGTTAACTCAATGGCAACTATTAATAACGTATCAATTAATTCAAATGAATTATCAAACAATTTAACCTACAATATTTTTGTAGAAGTTTGAAGAAAATTATCTAAAAAATATTCTAATTATTCAGAACTAATCGACAAATTATGTGAAACGGTTGAAATTTTTAGTTATTTTGCTAAAAACAAATTGATTAGAAATTCTAAAAATAAATATGATTGAGATTATGACAATCTTGAAAATATGAAGTTTTTAGTTAACTTTATCATAGGAAAAGGCAAAACAATTTATATCCCTTTACTTTGATCTATTTTTGACAAAATGAACTTATTTAATGTCAATTTGGTCAAAGAATCGAGAATTAGTTTACTAAGAAGATTATCAAAAGTAGTTTATAAAGTTGCTAGAATTAGATTCTTTAACAAATTTATTTCATTTAAAGGTGAATCACTAACAATGCCAATTAAAAAAATAGTTCAAAGTTTAAATGATGACTGTACAAACTTTGATAAATTAGCATACAATAATTTTGTATTTTTAAGAAAATATGCTGAAATTGATGAAATAAATAAAAAGATGGAACAAGATTTGTTAAATAAGAAAACAGATTTATTAGCATCTTCAGACGATCCAGTTTCTTTATTACTTTTAATAAATTATAGTATAGGAAAAAATAATAAAAATCTACACTATTTTGAAAATGATGAACAATCTTCTTATTTTAAAGATTGAACAAATTTATCTTGAGAACATTTAACACCAGTTTTCAGAAATAAAAGTGGAGAAGTAGACCAAGAAAGAAAATATCTAAACCTTTTAGGAAATGCCCATATAGCTAGCAAAAAAACAAATAGTTCAAAAGGAAATAAGAAATTTAGTGATAAATTTGGCGATTATATATTTGATAAATTATTATCATTAGAATTTGATTACAATACCAAAAAACAAAACCTAAAGGATGAAGAAAACGAAGAACATAACCTAGAGGATAAAACAATCGAAAAACATAACCTAGAGGATAAAGAATTTATTCAAAAGCATTGAGAAAATTTAGACGAACAAAAACAAATAATGATAAGTAGATCAAAAGTTATGTGAGAGCAAATTTATAAAGTTTTTGAATTTGATAATGAAACATTGAAAAATTTCAAAAATGCTAACTAGAATTTTATTTTAATATAAAATTAATTCATGACTTTTAAAAATAAATTAGAAAAAGTACCTCTTGGTTTAGGAGGACTTGCACTTGGCATAAGTGGTTTAGCTGGTGCTTATAATGGGGCTTTTAATGAATTTAAAAACTCAAGCAATGGAAAATTAATTAATAATGTTTCAATTGGCATTCAAGCTTTTTGGATGTTAATTGCTTTTGCTTTAGCTTTTATTGTTTGCTTAAGATTTATTAAAGCAAGGCACGTATTTAATAAAGAAGCAAAAGCTCCAAATACAGCAGCATTTATACCCACTTTATTAATGACATTTATTTCTATTTTTACTTTTATAGCCTCAATATTAAATACTTATTTAGAAGCTAGTGAAGCTAAAAAATGGTTATATATTCCATTGGTATTTTCATTCATTGCTTTTATATTACAAATAGCTTATTTAATAACTTTTATAGTATTAATTATAGTTCAACATGATTTTAAATATGATGAAGCTTTTGCTTCTTGATTTATTCCAACAGTTGGTTTTGTAATCCTTACTTTTGGAGCTAATAATTATCAAAATATAATACCCTTACTTTTTTACCAAATACTCTGATTTATAGGTTTATTATTCTGCTTTTTAATCTTTATTTTAGTAGCATTTAAGGTCGCTTTTGTAGGCCACAAAAATCATCAAGATATTCCATCTTTAGCAATTTTTTGTGCTCCAGCAATGGTATTAAGTTTTGCATTTTTAACTTTATTTGATCCTAAGAATCCAAACGCAAATATTTTAAATAATTATGTAATTTTATATACTTTTACAGGAATATTATTCTTATTTATTTTAGTTGGTTGAGCTGCATATTTTTATTCTTTAGCTATCTCAATAAAATCTAAAAAATTTTATTTCACTTGAGCTTGTTTTACATTCCCTATCGAAATAAGTTCAACAGCTTTATTCAAATATGCACAAGTGGTATTTACTAAGGATTTAAATGTTTATGTTCATTTTGCTTTTATTATTTTAGCTATCTTATTCTTAATCTTTGCAACAGTAATTATTAATTATGTTTTCTATAAATATGTTGTTGGTTTACATAAAATATTTAGCGATAAAGATAAAAACAATTCTGATATTCAAAATCAAAAAACAATTGTTGAAGGAATTGAAGTTACTAAAGTTGACTTAGATTCTTTAGAGTAAATTAAGTGTTCTTAGTTGGACACTTTTTTATTAAAAAAAGCACATAGAGTGGTTCAAATGTATTCTTATGAGGAAGAATAACGAATTTTATCTATTACATATTCTGTAAAGTCAATTTTATTTTGTTTTTCGAATAAAACATTATTTATATATAAATCAAATTTATTATCACTTCTAGTTTTTTGAAATTTGTTCGTTCCTTTAGTATCTGAAATTTCAAATTTTTTGGCTGGAATTTGCAAAATTGTTATTTCTTGAGAATTTATATCATTTAGCACTAATTTTAAATCACTATTAACTGAATTATTTTTTAAAGTAAATCAATAATAATTTTTACGATCATAATATTTACTATAGTAAGTATTATTTTTTAAATCAATTTTTGTTTTTGTTTCAATATAAGAAAATATTTCCTGTTTACTTCGACTTCTAATTTCTTCTTCTGATTTGGGTTTAATAATATTTGATAAATTTACATTTTCTAAATTATTTTTCTTGTTTGTATTATCGTTAAAAACATCAATATTAATAAAGTCTAATAAGAAAATTAATTCATTTAAATATTCTTTTAATTCTGTTTCTCGGAATCTATCAATATTAGTTTTGTTTCCATTTTTTTTATTATCTGAACTTAATTTATTATTCGCTTTTGCCTTATCAATTAGTTTTGATTCTAAATAATCGATTCCACTTTTGTCTAATGAATTATTTGATGTAGTAAATAAGAAAACTTTTTCTCATCAATCTTTTCCTAATAAATGTTGTTCAATTCTTTGTTTCAATTCTGAAGATTGTCCTACATAAACTTGATCTTTTGCAATCAATAAATATATTCCTCAATTATTGCATTCTTCATATTCGTCCAATAATTTAGTAACCTTATCGCGAGGACTTACCAACATAATTCCTCCTAGAGGCCATCTTAAAGTTTTGAGATACAAAATCCCGTCTAATGTCCCATCTTCTAATATTAATTGAATGGTTGTTGTTAAAGAATTTTTATTAGTATTTGTTGTCATGCTTAAATTATCGCAGATTAGCAAAAAAATGTTACTTTTTTTATAACTTTCAACTTTAATAACCTAATTCATTTTGTTTGCATAATATAGAAAATGTTATATCATAATAATATATAAAGGAGCAAATGTGAAACTATCGATCAAAGAAATTATCAAGAGAATTAAAATTCTTGAGGACGAATTAATTGTTCTTAATCAAGAAGAAGATAAAGATAATTATGTTATATATTTAAAAGATGAAACAGTTGAAAAATCATCGTATAATTTTAATAAAACTAATAAAAAACGTCAAGAAATTAATCAAGAAATTTTAAGACTTAAAATTATTGTTCAAAAAGCAAACATCAAAACAATAACTAGTTATAAAGGATTAAGTATTTCTGAACTTTTAATTTTATTAGCACAAAAATCTTCTTTAGTTCAAAGATTTGATTTGTTATGTTCTAAAAAGCAAAAAACAAGAAAAACTACCAACGATGGTCAAATTGAATATATTGAATATTTATATGATATTAACGAATTAAAAAATACTAATTTAAAATTAAAACAAGAAATAGCAACAATGCAAGTTGCTATTGATAAAGCTAATATTGAAACATTAATTGAAATATAATAATTTATCTTTCCTTAATAATGCTATTTATACTTAAAATTACAATTTAGATTTATTGTTATTGAAAGTGTTTAATAGTTTTAGTTAGTGCTTATTATTTTATTAATATATTTTTAATGTTTATTGTTTGTGTAAAAATTATTCATAAAAATTAAAGCATTATTTAAAAACTTCAAATATGGAAGTAAAGGGTTATACATATTTAGCTACATCTATGATGTGGCTTTTTTATTCTAAATTTAAATCAGATGCTTTTACTCCATTTTTACTTTTCATTTTTTCTAAAATAGTTTTAAGTTTATTTAATAATAATTCAATTCTTAATTTGTCTTATGGAAAAGATTATTTTATAGAAAATATAGATAATCCAAAATTTCTTAAAATATAAAAATAAAACTATTTTTTTCTAATTTGATCTGTAATTTTAACTAAAGCCTTAGTTATATTAACTTCTCTTTGTGAATGAGCTGCTTTTTCAACCATAATTAAATGCGAATTATTTAGTTTTTGATGAAGTTCATAAGCACCACTTGGGCGACAGTCCAAATCAAATTGACCGTGAACAATGTAAGTTTCAACATCTTTAATTTTGTTAATATTATTTAAAATATAGTTTTCTTCGAAGAAACATTTATTATAGAAATAATAATTTTCAATTAATGAAATTTCATATATTCACTTTGGTTTTTTAGGTTCTTTAAATGGTTGTTCTTTAAGCAACACTAAAGATGATTCTCATCTCGTTCATTCAATTAATGCTTTATGTCTTAATTCTTTATTTGTTGAATGTATATGTTTATAATAAGCATTAACTATATTTTTTCTTTCTTTATCACTTAAAAATGATGTGTATCTTTCATAAGCTTCAGGTTTAAAGTAGCTAGCTCCTTCTTGATATAATCAGTCGATGTCACTTTGACGTCCCAAAAATATACTTCTTAAAATAAGTTTATCAACTTTATCTGAATGATTAATTGCATAAGATAATGATAAAGTAGTGCCCCATGAGCCACCAAAAAGGATTCATTTATCTATTTTTAATTCTTTTCTAATGGCTTCCATATCATTGATTAAATCTCAAGTTGTATTATTTTTTAACTCTAAACTTGGAGTACTTTTTCCGCAACCTCTTTGGTCAAATAAAACTATTTTATAGTATTTTGGGTCAAAATATCTTCTACATACTTCACTAGTTCCTCCACCAGGACCACCATGAACATAAACTATTGGTTGACCTTGTGGATTGCCACTAACTTCATAATAAATTTGATAAATATCATCTACTTTTAAATAACCTTTTTGATAAGGTTCAATTTTTGGATATAAATATTTTTTGTACATGATTTAATTGTATTCCTTTTTCTAATTTTCTGGTTTTATTCCTAAAACAGATCATTTTCCTGTTTTTCTTGAACCAATTCTTTTTAAAATTAGTTTGTTTTTTAAAGATGCTAAAGCTCTTTCTATTGTTCTTTTAGAAACTTCTAATTCGTTTGACAATTCATAAGCTGTATATTCTTCATTTTTTTGAATTAATTCGAATATTTGATTTTCGATTTTGTTTAATTTGTCTTTTATTTCTTTAATTTCATAATTGTTATTTTTTTGTAGCAATTCCTTGTTAAAAGGTATTGTTATCCTGATATAGTTTTTTTCTACTTCAATTGCCATTTTTCCATATTTTTTTAGAATAAGTGGAACGCCATGTCCGGTTTGTTCTGCCAAATTAAGATCTATAAATATTTTCATTAATTTTGGATTTCTTGGCTTACTTATTCCTTGATAAAATTCTTCAAGTTTCAAACCTTTTGGTAATCCACCATAAGACATAATTTCAATTCTATTTTCAAATTGTTGGATAGTTGGTACGCCACTCAATCAATCATTATGTACAATTGCATTTAATAAAGCCTCTTTAGCTACATTAAAATCAAATAAAGTCTTTTCAATTCTTTGCATTGAACTAGTATCTACATAGCATTTATTTTCAACTTCTAATCTTGCTAAAATTTGTGAATAACCAACCAATATACTTTTATTACTGAATTTTTGTATTTCTGAAAAGACTGTTTTATCATTTCCTTGAAATTTTACATATTTCAAGTCAATTTTATTTTCATTAGCTAGCAATTCAGCTAGCTTATTGTAACTACCTAATTTATTTCTTAAATGTAAATTTTTTTCAAATGTTTTATCATCTAAATGTAAATTATTTAGTTCATAATTTATTTTTAATATTGAAAATTTCAAATCATCCAAATCACTAACATAATTTATTATGTTGTCCTCATCATAAAATCTAATTTTGTTTCTTTCTTCTATTTCTTCATTTGACATTTCTCTTGTGCTTGTCCCAACTCTTACTAAACATCCTTTTGAAGAACAACCATATTTTTTTATGTAATAAATAGCTTTTTCTCCTTTTTGAACGTTTATTCCCATAATCATTTTGTTATTTTCAAATTTTATTTCACTTTTTACTTCTTTTTCCGCTCTTGGTTCAATTTGATCACAAATAATTTCGTTTAATTAAGTAAAAGCTTTATTTACATCTTTTATGCCAATTACTTCACCATTATCTTTTACACCTATATAAATTGTTCCGCCTTCTGTGTTTAAAAAGCCAACAATTTCTCTAATTAAGTTATCATTTAATTTTTCCTTTAATTCGATTTTATTTGTTTCAACAAATTTCATTTTTTACCTCTTTATGAAAATTATATACTTTTTAATTTAAACCGCCAAGTAAAATGTCGGTTTATTTGTCGGTATAAATGTCGCCATAAAAAATAGACCTTAACATCATGTTAAAGCCTATATATTATTCTTTTTTATTGTCTTCTTGTTCTTCTTTTGGATCATTTTCAACAGTATTATTTTGACTATTGTCTTCTTGAATTGCATCTATTATTTCTTGTGCTTCTTGGTGTGTATCTTCTCTTCTTTTATCTAATATCATTTCAATCACAAAGAAGAGTACTAATGAGATGCAGCCTAAAACAATACATAAGTCTGCGAAGTTAAAGGTTCCTAATACTTTGCCAGTTCACTTTTCCATAAAAGGAACATAGAAAATATCTTTAACCATCATATTAAACATAAAGCGGTCTAACATGTTTCCAAAGTCACCGGCTCAAATAATTGCACAAATAATAATAGTGGTTAAGTGTCTTGATAAAAGTGGTACTAATAATAAACTAAGTAAAATTAGGATACTTAAAAATTGAATAAAGATTAAAGCACCTTTTTTATTTTCAACACTTGGAATGAAGGTTACTCCATAGTGACCTACAGATCTAATTCCGAGTAAAGTTCCTTCATAATAAACTTTTCCTATTTTGCCACCATTATGTTCTTCATTTCATTCGAATAAGAAGGTTTTAGTTAATTGATCAATTAGAATGGCAACAAAAGCAACAGCAAAAAATATTCCAAAAGCAATTAAGATTTTCTTTTTGTTATTTTTGCAGTAGTTAATAAAATCATTTCATTTTTTACTAATTGTATTTTTTAATGGTTCTTTTTCTGGCTCATAGCCATTGTATTCTTTACTCATTAAAAACTACCTTGTGACAAAGAGGACAAAGTTCCTCTTTAATTTGTGAAGGCATGAAATGATTTCAACATCTTTGGCATTTAATTGATTTGAAAACTGAAACAGAAGTTTGAGCTCCAAATTCAACTTTACCAACCATTAATAATTGTCTTAAATCAAGACTTTTAATAAAGTCGCTATCGCTTTTAATTACAACTTTAGCTTCATTAGTTCTTTTAATTAAACCTTCTTTAATTGCATTTTCAAGTGATAAGTTTACTTCATCACGAAGTTTAAAAAAGTCTTGCCATTGAGTTAAAACTTGTTCGTTAACTTTGAGTGGGTTAGCTAAAGTTTCGAGATGAACACTTTCAGCTTTATTTACTTTATTAAAGTTTTTGTAAGCATCTTCAGCTGTAGTTGGAAGAATTGGAGCAAGAGCAATAATTAGAAAATCAACAATTTCATAAATATTTGTTAAAGTCATAAGACGGTTAATTGAATCTTTAGCTTCGACATATAAAACATCTTTTGAAATGTTTAAATAAAAGCTTGATAATTCAACTACATAATTATTTAAAAGTTTAATAACGTTAATGAATTTATATTCATCATAAGCTTCAATAACTTGAGCTTTAACTTTTTCCAATTGCTCTTTAATATAGGCATGTACGCCAATTCTTTTGAGATTTGAGTCATATTTAAAACCATTTAAATTACCGAGCATAAATTTAATTGTGTTTCTAATTTTACGGTAAATTTCGCTGTTTTGATTAATGATATTATCACTAATACTTACGTCATTTGAGTATTCAGAATTGGCTACTCAAAGACGTAAAATATCAGCTCCATTTTTGCTAATTATTTTAAGAGGATCAACAACATTACCTTTTGATTTAGACATTTTTTCACCTTTAGCATCTAAAACAAAACCGTGTGAAACAATGTTTTTGTATGGTGCTTTACCATTATAAGCTACTGAGTTAATTAAAGAAGAGTTGAATCAGCCTCTAAATTGGTCACTACCTTCAAGATATAAATCATAAGGAGTTTCTAAACCTTTATCAATATTAACTGCCATAAATGTTGAACCTGAGTCGAATCAAACATCCATGATGTCGTTTTCTTTTGTATAACCTTTGTTTTGGTATTTTTTAGGTAATAATTCATCAGCAGTTTTTTGATATCAAATATCAGTTCCGTGTTTTTCAACAAGCTTAATAATGTGATCAAAAATGTCTTCGTTTAAAACTGGTTTTTTACTTTTATCATAGAAAATGATAAGAGGCACACCTCAAGTTCTTTGACGACTAATAGTTCAGTCATCTCTATCAACTATCATGTTGCTTAAACGTTTTTTAGCTCAATCTGGATAAGTTTTAACTTTTTCAAGTTGGCTTAAAATTTGATCTTTAATTTTATTTATTGAAACAAATCATTGAGGTGTTCCTCTGAATAAAATTGGTTTATGAGTACGTCAGTCATGTGGATATGAGTGAGTTATTGAGCTTTCTAAAAGTAAGTTTTTAGATTTAGTTAATTCTTCAATAATTAAAGCATTAGCTTTTTCATAAAATTGACCTTGGTAAGCTCCACCTTCTTTATTAATTTTTCCATCATCTTCAATATGCATAATCATTTTAAGTGAGTTTTTGATTCCAATTTGGAAGTCATCTTCACCAAAAAGAGGAGCAATATGAACTAAACCACTACCACTTTCTAAAGTAACGTGGTGGCCTATAACTACAGGACATACATTTTTATTAAGTGGGCTTACATATTGCATATTTAAGATTTCTTGGCTATTAAAAGTGTTAGTGATTTTGTAGTTTTTTCAGCCTAATTGACTAACTACTTTTTCAACTAAATCAGAAGCAAAAATATAGCCTTTTTTATCATGTTCTACTCTTGAGTATTGAATATCTTGGCCAACTGCTACAGCAGCATTAGCAAGTAAAGTTCAAGGTGTAGTAGTTCAAATTACTAAGTTATCTTTAACATTAATTTTTTGGCTACCTAAATTATCTTTAATTTCAAATGCCACAAAAATTGAAGGTGATTTAATATCTTTATAAATAACTTCAGCTTCAGCTAATGCTGATTGACTTGAAGGTGATCAATAAACAGGTTTTAATCCTTTGTAAATAAGACCACTAAAAATCATCTTTTTAAAAAGTCTTAATTGTTGAGCTTCGAATTTAGGATCTTTAGTAATGTAGTATTTTTTAAAGTCACATAAAAGTTGCATGCTTTTGAATTGTTCAGCTTGTTTATTAACTTGATCTTGAGCATAAAGAGCAGCTTTTTTACGTAAGTCAACAACACTTAATTCATTTTTCTTTAAGTGTGCTTCCTCAAGCATTTTGTGTTCAATAGGAAGACCATGCGTATCTCAACCTGGAACATATGGAGCATAAAAGCCTCTTAAAGATTTATAACGGATTACAATATCTTTAAGGATTTTATTTAAAGCATGTCCGATATGCAAATTACCATTAGCATATGGTGGACCATCATGCAAAATGAAAGGTGTATTCTTTTTGTTTTTATTTAATACTTTTTCATAAATTTTATTGTCTTCTCATTCTTTTCGGAATGCTGGCTCTTTAGTTGTTAAGTTAGCACGCATATCGAAATTAGTTTGAGGCATATTTAATGTTTTTTTGTAATCAATTGCCATATATTGACCTCCTATTTTTGATTTTTAATAATTGCTTGGAAGAAATTATCAAAAAGTGGATTTGAAGCTAAAACTCTAGTACTAAATTCTGGGTGATATTGAACACCTAAATAGAAAGGATGGTTTTTAACTTCACAAATTTCAGCCACTTTTTTAGTTGGATGAATTGCACTGAATGTAAATTCATCATCTTGTAATTTTTCTAAGTATTCAGGATTAATTTCATATCTATGTCTGTGACGTTCATAGAAAATATTTGAACCATAAATTTTTTGAGCTAAGGTGTTATTTTTAGCTTGAACTTGATCTTCACCAAGTCTTAGTGTGCCACCAATAGCCATTTTGTCTCCATTTTCATAAAAAGGAGTTAAAACAAAAGTTTGTTTTTTACCATTTTCAGCGAATTCTTTGCTGGTTGCATCTTTAATGCCTAATAATCGAGCTTGAGCTACTGACATTGCTTGAAAACCTAAACAAATACCTAAAGTAGGAATTTTGTGTTCTCTTGTGTAAGTCGCAACATTAACTTTAGATTCAAAACCTCTAGCACCAAAGCCTGGTAAAATCATTACTGCATCATGTGCTTTTGTTAATTTTTCAATGTTTTTATTGTTAACTAAATCAGCATCAATTAAGTCATATTTTAATTCGACATTTTGATGAACAGCAGCTATTTTTAATGAAGAAATAATTGATAAATAAGCATCTTCTAATTCAGTATATTTACCAACTAAAAGTAAGTTAATTTTATAATTTTTAGTTGCGTGATATTTATTCAAAAATACTTTTCATGAATCATAAACCTTGTCATTAATTTGTTTTTTAATTCCAAAATGTGAGTAAATTATTTTTAAAATATTTTGTTCATATAAGAACATTGGAATTTCATAGATACTGCTTTTATCAGGAACTCCAATTACATGATTAGGGTCCAAAAATGAAGCTTCAGCAACTTTATTAATAATTCTTTCATCAACTTCGCCTTGACTTCTTAAAAGTAAAATATTAGGGTTAATTCCAAATGATCTTAAAGAAGCAATTGAAACTTGAGATGGTTTTGATTTGTATTCTTTTGAAGCAGTTAAATAAGGAATAAATGCTAAGTGAGCAAAGAAAACACTTTTAGGATTTAAACTAGCAAACTTACTAATTGCATAAATATAAGGATTACTTTCAATGTCTCCAACAGTTCCTCCGATTTCAATTAACATGAAATCTGGTTTTTGTTTTTTAGCAGTATTTTCGATAATGCTAATAATTTCATCAATAACATGAGGTACTATTTGAACAGTTTTTCCACCATAAAGTCCTTGACGTTCTTTTTCAAAAATGCGAGTAAAAATTCTTCCACTTGTGTAGTTACTTTCCTTGCTTAGCTTAACATCAATAAATCTTTCATAGTGTCCTAAGTCTAAGTCAGTCTCACCACCATCAGCAGTTACAAATACTTCACCATGTTCAATGGGACTCATTACTCCTGGGTCAATATTTAAATATGGATCAAGTTTTAAAGCAAAGACTTTAAAGCCTTGTGCCTTTAATAAATTTCCAATACTTGCACTGGTAACACCCTTACCTAAACCAGATAAAACTCCACCAGTTGTCACAATAAATTTAGTCACGATAATTCTCCTTAATACAATAGGATTTAAATAGTTATAGACTTAATTATAATATTAAATATTTATATTAATAATAAAATAATTTTTTTGTTTATTTTTGTTCTTCACTGATTTTTCCTAAATAACTGTTTTATAGTTGTTTTAGTTAAAAAAAAGAAAAAAATTGTGATTTGAAATCATTATTTACAAATTATTTTCTAATCAATTATTAAAACAGGTTTTTTAGTCAAAAATGATTACATTTTTAAATTACTTTTATGCAAATTATATGTATAATTAACATACTAATTAATTGGAGGAAAATATGGATTTATCTAAAAAATTTGTAGCACAAAAATTAAACTTAAAAGAAGACCAAGTTGAAGTTGTTCTTAAGTTAATGGATGAAGGAGCAACAGTACCTTTTATTGCCCGTTATCGTAAAAATGTAACTGGTGGACTTGATGAAGAAGTTATTCAACAAATAAGTGACATGTATGTTTACAATGTTGAATTAAACAAAAGAAAAGAAGCTATTATTGACATCTTAAAAGAAAAAGGATTACTCACACCTGAAATTGAAAATAAATTAAAAGCAGCTGAAACTAAAGCTGAAGTTGAAAACATTTATGAACCATTTAAAGTTGGTAAAAAAACTAAAGCTAGTGAAGCTATTGCTTTAGGTTTAGAACCTTTAGCTAAAGCAATTATGGAAGCAACTGATGAAAGTTTTGATCCTTATAAAGAAGCTAAAAAATACTTAAATGACAAAGTACCAACAGTTGAATTTGCTATTGAGCAAGCTCAATACATTATTAGCCAAATTATGTCTCAAGATGTTGAAAATAGAGAATATGTTAAAAAACAAATTTATGATTTTGGTTTCATTGTAACAAAAGTTAAAAAGAATGCTGAAGATGAAAAAGAAGTATTTCAACAATACTATGATTATAAAGAAAGAATCAAGTTTATTCCTAACCATCGTGTTTTAGCAATTAGTCGTGGTGAAGACAAAAAGATTTTAAGTTATGACATGACTTATAATTTAGCTAAAATTACTTATGACTTAAACAACAAATATTTTAAAATCAAAAGAACAGGCAAGATTTTGAACGAATCATTAAAAGACAGTTTAGATCGTTTGATTTTACCTTCAATTATTAGAGAAATCAAAAGTGATCTTTTTGCTCGTGCAGAAAAAGAAGCAATTAACCTTTTCGCAACTAATCTTGAAACAATGTTACTATGACCTGCTGTTAAAAATAAAACAGTTATGGCAATAGACCCTGCTTTTGTTAATGGTTGCAAAATTGCAATCTTAGATCCTCAAGGTAAGTTCTTAAAAAAAGCTTTAATTTATCCTAACCCTCCTCAAAATTATGTTGATAAAGCTAAAGAAATAGTTAATAAACTATTAGATTCATACAAAGTTAATATTATTGTTATTGGCAATGGAACAGCTTCAAGAGAAACTGAAGAATTTATTGCTAATTTATTAAAAGAAAGATCAAAAGTAAATCCTAATGAAGATATTTCTTATGCTGTTGTTAGTGAAGTAGGAGCTAGTGTTTATTCAGCTTCTAAAGTTGCTATTGATGAATTTCCAGATTTTAGTGTTGAAGAAAGATCAGCTGTTAATATTGGACGTCGTTTCCAAGATCCTCTTAATGAATTAGTTAAAATTGATCCTAAAGCTATAGGGGTAGGTCAATATCAACATGATGTTAATCAAAAAGATTTATCTAAGGCTTTAGACTTTAAAGTTGATAAAGTAGTTAACATGGTTGGAGTTGACTTAAATAGTGCTACTAAAGAAATCTTATGCTACATTTCAGGTTTAACTAAAACATTAGCTCAAAACATTATTGATTATCGTGAAAAAATCAATAAATTTAAAGAAAGAAAAGAACTATTAAAGGTTAAAGGTTTAGGCGAAAAAGCCTATGAACAAGCGGTTGGCTTCTTAAGAATCCATGATTCTAAAAACTTTTATGACCGCACAGCTATTCACCCTGAATCATATCCTTTAGCAATTAATATTGTTAATGCGCTTCAATTAGATCTAAATGATATAGACTTAAAAACTATTGAACAACAAGATGCTAATAAATTAGCTAAAGACTTTAATTCAAATGAATATGATGTTAAGTTAATATTAGATTCTTTATCTAATCCAACTAAAGATATTCGTGATGACAAAGATGGCTACATTCTTAAAAAAGATGTTCTTAAGTTAGAAGACTTAAAACCAGGTATGATTCTAGATGGTTCAGTGCAAAATATTACAGACTTTGGTATCTTTGTTTATATTGGAATTAAGCAAGCTGCTTTAATTCACATTTCAAATATGAAAAGAAATAAAGATCACTACATCAAACACTCTAGCGAAGTAGTTAAAACAGGAGATAACATTAAAATCCAAATCCTTGAAATAGATAAAGAAAGAGAAAGAATTCAAGGCAAATTAATTTGAGAAGATTAATAATCAAATCAAATAAGCAATTTTTGTCAAAGCATTGGCAATATTGCTTATTTTTTAACATAATAGCATATATGAATTTTTATAAAATTTAACAAAGCAAAATGTGAAATTTAAAACGGATTGTCATACTTTTTTTCATATTTTTTTGACATTAATTTAATGATACAAAATTATTAAATTTTTGTCAATTTTTTTCAAAAAAACATGTTTTTTTATAGGAAAAGTTAAAAAAATAAAACAGTGTTTAAAATTAAAAATTTAATTTTTTAGTACAAAAAACTAACTCTTTCTTTTTAAAAAGAGTATATTTTTGTCATCTGAAAGAAATAATTGAAATTAGAATTGAATTAATTCACCTATTTTTTTATTGATCTTACAGATGTTATGAGTAAAATCTTTTATTTCGCTTTCTAATTTATTTTTGTCTAAATTTAGAGTGAGTAAAAGATTTTTTAATTTTTCAATTTTAGATTCTATTAAGTTATAAAAACTATTTGTTGATTTGATTTTTGTATAAAGAAGTTTTGATATTGTGCTTTCTGTCACACCTAAATTGTGTACAAAAAGTTGATATTCGTTAAGTTCTTCTCTAGTCAATGTTTTAGTAGGAAATTTTTCAATTCCTAAAATGTCGATATAAATACTACCATCCTTATCAATAAGCACATATACTTTTGATCTATCATTTAGAAAAACTCTTTTACCATTTTCTGGATTGTAAGGATAATAATATTTGTAATCATATTGTATTGTGTTAGCGGTTACTATTCTTATAAATCTCTCAATGATTTTATAATCACCATTTATTACTTTTCCAAAATGATTTTCTTTTACTTCAATATTTTTTCTAAGTTTTTCATTAAAGTAGTTGTTAAGGTCATTTGCAATTTTATTAGATTCTTCTAAATTTTTACCATTTAGTATTGGTAAATAATTAGCTTGAGCTGTTTTGAAAGATCTTTCAACTTGTACTTTAAAAGTAGGAGAACTTGAAGATTCTACTTTTATTTTTTTCCATTAAAGTATTAGTTAAAGGGGTTGTGGTGTTTTCAGAAACAAAAAATTCTTTTCTTTTATTAGTTCTAATAACCTTATAGTGAACCACAAATTCCGGACTTTTTGGAAAAAGGTTCGCTCTAATATATAATATTATTAGTTGTTAAAACAATACTAAAATATTAATAAAGGAGAGTCTATATGAAAGAATATAAAATTTTAACAGAACACCCATCAAAAGCTGAAGATTTAATGAATGAAATGGCAAGTAAAGGTTGAGAAGTTATAGCAGTAAATTATTGAATGAATTGAAAAGTATGTCTTGTCATAACATTTGTTAGAGATAAACAATAATAGAAAAGATCTTCTCATTATTTAGGGAAGATTTTTTGTACTTTTATTTAATAAATTAAGAACGAAACGGCTCCTTGTTTGTTCTCTATTTGTTCCTTATTTGGTATAATATTTACATGAAAAAAGAATTAGAACTATCATTTAAATATACTGATGAGCTTGTCAATTATCTTCTAAAAATTGAAAAATACAAAAGTTCATTAGAATTTTTGTCTTTACCTACAAGAAGTAAACAAAGAATTCAATTTGAAGCCAAAATTAAACAAACTCATTTTTCAACAAGTATTGAAGGCAATGTTTTAAACATTGAACAAGTTAAAAACGTTATAAACAATAAAACACATCAAACTAGAATTAAAGCTGAACAAGAAGTATTAAATTATTGAGAAGCTTTAAGTTTCTTAGAAAGAAGTAAAAAAGCTAAAATAAAAATAACTAAAGATTTTATTTTTAGTTTACATAATATTATTGAAGCTAAAAGTTCTAGAATTACCAAAATAGATTTTCGACAACCTACTCCTCTTGGAGTACTTTTTGCTGTATATGATTCAGTTACTAAAAGTCCTGAATATATACCACCAGAAGCTAAGGATATAAATATTCTAATAGATGAATTAATAACTTGAATAAATGAAAATCAACATTTACCAAGTGCAATAAGAGCAGCTATCATTCATTATGCATTTGTTACAATTCACCCTTTTGAAGATGGTAATGGTCGTAGTGCTAGAGCACTAGCTACTTATTCATTAATGTTAGATGATTATGATTTCAAAGGATTTAATTCTTTTGAAGAATATTATAGTAATAACTTAAATGAATATTATCAAGCATTACAAATGGATTTAAGTCCTTTATTTTATAATGGACGTAATAAACTGCCACATTTAGAAAAATGAATAACTTACTTTTGTAAAATTATGGCTCTTAATGCACAAAATATATATTTAAGTGCTTTAGAAGCAAGCACTAAAGATAATAGTTATAATGATTTGTTAAATAATCTTAATAAAAAAGACTTAACTTTATTAAGATATTGCATAGAAAATAAAAAAAGTATTATTCTTATTAAAGATTTAGCTTTAGTCTTTGGCGTTACTTCTAGAGCTATATCAAAATGAACTAAAGACTGAATAGACAAAGATATTTTAGTTCCAAATAGTGGTACTAAAAGAATTGCTTCCTATAAATTAAATAAAAAGTATGCAAAATTTAAAGTTAGTGATTTAGGATTTATAGATTAAAAATAAATTGAACTATAATGTAATGGAGTGGTTAAATTTCACAAAATTACATTATTTTTATTTGGATTAATTAATCAAATATAAAAACAATGGATTAATTAAAGTTATTACTGGTATTAGAAGTATTAGAAGAAGTGGCAAAAGTGTTTTATTAAATAATTTTTTTATAATTACTTAATTTAAAATAACGTAGATATTAATCATATCATTTCTTTATTATTTGATAACCTTGAAACCAAAGAATTATTAGATGCCACTAAGGCATATAATCATATTAAAAAATTAATTAAAGATCAAGGAATATATTATTTATTACTTGATGAAATTCAAAACATTAAAGACTTTCCGTTATTATTAAATTCACTTTTAGATTAGTCAAAAGTCAAGTTGACATTTATGTTACTGGTAGTAACTTAAAATTTCTTTCAAATGATATAGCTATAGAATTTATAGGTCGCGGTGAAGAAATAAAAATCTGTCCATATTCATTTAAAGAAATAGTTCAATATTTCAATTTAAAAGAAAATGAATATGATTTATATTTTGATGATTATTATAAATAAGGTGGTATGTTATTAGTTATTTTAAAAGAAAACATCAATGACAAACAAAAATATTTAAAAGAATATTTAATAACATTCTTATTAAGTGATATATTAGAACACTATAATGTCAAAAATGACTATAAAATAGAGGAATTATTAAAATTCTTAGCTTCTAGTTCTTCATCTTTAACTAATACTAAAAAATAAGTAATTTATAAATAGTAATAACAATTTTAAAATTGATAGTAGAACAATAGATACTTATTGTAACTATTATTAAGAATCATTTATTTTTAAAAAAGTTACTCAATATGATATTAATGGTTTAAAAATATTAAACGATAGTAATTCAAAATATTATTTTATCGATAATGGTTTAAAGAATGCTTTAAATGATTTTAAATTCGATGACTTTGGTCGAACATAGAAAACATTGTTTACTTTGAATTATTAAGAAAAAGGGAACTAATATAAAAAGGTGTGTTATAAGATTCTCTACGTTTTCTTATTTCGTAATTATTTTATCATAATCCATTTTGTAGCAAAGGAAAATACTGCATGTGAAGAGCATGCAGTTTCCTTTTGCGCGCACACGCGCTACTTGAATAGTACAAGATAAAGACACTACAAAGCTATTTACCATAGCGTGTTTTATCTTGAAATGATTTATTCATAGAATAAATCAAAAATTAATATTTTCATATTTTCTAGGCGCATGCACTTTTTCGTTTGCCTAAATCGCATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATGGATGTTTCATAGATATAAACAGATTCAATAGTATTATACATCATTCGAAAAGATTGATTACAATCTGTGATTTAGTAACATTTAAGCAATAATACTATAAAACAGTGTTTTTGGAGAAAGGATAGTATGAAAAAATTAAAAAATTTTTTGCTTGGTATTTCATCTACAATTCCAGCTTCTGGAGTATTTTTGATATCAAGCACTAATTCTAAAAATGAAGATAGTTTTAATAATTGAAATGAAAGTATTAAAGATAAATGTCCTATAAAAAATGCTAACTGAGAAGAAATATTTGATATTTTAAAATCTAAATTACCATACAAAAAAGATATTGAATTGGTGTCTTATTCAGGCATGAAAGATAATTTAAATTCAAAATTAAAACTAAATAAATTCAGTGATAAAAAAACTCCTAAAGAAGAATTACTTCAATATTGAAAAAATCTTGACATTAATTTAGCACTAAATAATTATTTGAAAAACAAAAGCATTAATAAAAATGAATTTTTTAAATTTGATTTTGAAATTGTAGAAAATCAATATTTAAATATTTATTTAATTAATGAAAATGTTAGTCTTAATAAAACAAGTAGATATTTATTGATTAAAAATCAAAAAATAAATTGAAAACTAAGTTCTGATATTTCACAATTAAATTGAAAAGAAAGATTAATAATAAATTATGGGTATAATTACAAAATAAATAATGATGGAACAATACAAATAAAAGAATCTAAATCAAAAAATATAACTTCAAATGAAATTATTTGTGAATATCCACCAACAGAAATTTATTTTGTTTCACCACAAAATTTACAGGAAAAATTTGAAATTAGAATTGAAAGTGAAAATGGTGAAAAAATTGTTTCAATAAACAATAATGAAATTTTTTATAGTGTTATTGATATAAGGAAAATTGTTAAAAATAAATTAAATTTTTCTAATAAGCTTTTCTTAATACACGAAGATTCAAATGGCATTAAAGAAAAAATTCAAATTAAATTTGAAAAAAGCGATATATCAAACACAATTGATTTAAAATATCCAAATTGAAATCCTGATTTATTAGAATACAAACAATTTATAAGCAAAAATGAATTAAATAAAGATGGAAATGAAATTAAAGATGCTAATGGGAACCCTGTACCATCAAAAAAATATGACCCTGGGATTGATCCTAAAACAGGACTAAAATCAGAAATTGTGTGATTAAATTTTAATGAATTAAACTGAAACCCAAAACTTAAATTAGATAACGACTTATTTTATGCAAATGACTTTTACATTGACTGAAAAAACATAATAAATTTAGAAAAATGAGTTAGTTATTATTTAAAATTACATAATATTGCTTTTGATAATTTTGATTATATTTCAAGCGACTTATTAGGATATAAAATTTTAGCCCCTGAAACAATAAAAAAACTCTTAAATTTAAAAATAGATGAATTAAATAATTTTTTGCTAAATAACTTAGAAAAATATTGTTATGCCATACTAACAGAAAAAGAATCTAAATTTCTTTGAGAATTTGTAAATGATAAAGAATTTGGTTGAAATTTATTTTTAAGTTGATATGAAGGTTTAAAACATGAATATGATAAATGACACACCTGAGATGAATCGGTGTTTGGCCCATATCCCTACGATGGATATTATTTTGGTAAAAATGTTCCTGATGAAGAAATAGAAAAAAATATCGAAATTCTATATGGGCTAATAGATTTATATGACAAAATAAATAAAATTGACTTTCTTCAAGACGAATCAGTAAACAAAGATAGTTATTCAAATTTAAAACAATTAAGAAAATATAAAAATCATTTTGATTCAAACTTTGCTTCAAATCCTTTTGGTTCATTTTATCTAAAATATAAAAATAATAACTCATCTGTATTAGCAGAAGCTATTAAGACTCAATATTTAACTATTAATGCAAATTCAAATATTTTAATAAGAGAAATAAAACAAAATTCAATAGGAAAATGAGAATTTGTAAATGATTCAGAAAATATGGAAATATCTAATTCAGCTCCTGGGAAAGAAATCAAATTAACTAAAAATGGTTTTTATTTAATATATGACATATCACTAGATAAAACAAATATTAATAAATTTAAAATTGTTTGAATAGATAATAATAGTACTACATATACAAAACCTAGTTATTTAACAGAAGAAGCAAATTCTCCATCATTTATCAAGAAATTTTGAAGAAATAAAAATCTTAAAGTTTCAAATGATTTCGAAAAATACTTAGCTAAGAAAAAAATTATTGATTTAAACATAACAAAAAAAGATCTTGATAAATTAAAATATGATGATATTTTTAATGAGTATAATAATTTTTTAATTGAAAAATCTTTTGAAGAAAATTTTGAAATCAAACCAGAAATTAACATTAACAAATTAAGACAAGATTTTAAAACAAACGAACAATTTTTAAATTATATAAATCAAAAAGATAATGAAGCTAAATTTATTGAAAATTATGTTATCTCAAAAAATAAAGAATTTTTAACAATTAAAAACTGCTATTTTGATTCAAAAAGCAAATTAATTAAAGTTGTTATTGATTTAAAAGAAAATGCTAGCTTAAATAGTTTTTTTATCAGAAGAAAAAGCTTTAATATCAATTTAGAATTAAAACCTATTTTTATTAAATTTAATAGTATTAATATTGAAGAACATATACCATTTAATGTATTTGTTAATGAGTTAACTAATAATAATCTTAATAATTTGATTAAATCTGCTAAAGATATAAATAACAAAGACATTGACTTGAATATTATCGAATTAAAACTTTTGAAAGATAGTGATGAAAATCAAATAATAGTTAAATCTATATCAAAAGACAAAAACTATTATATAGCTAATAATGATACATATACATACAATGTAAGTTTACTTAAATCAGATTATAGTTATTTATTGAATTTTAATCCTGGAACTTTAAATGTTTTTGGTTTAAAAGATAAAGAAGAAATCAAAAAATACATTAAAGAACTATTAAAGAATTATTTAATCAAATACAATGAAAAAATTGATCAATCTCTTTACAAAAAAATCACAATAGATGATATTAATATATCGTTCAATGATGAAGAGTTAAACAAAATAATTAATCCAAAAGACGATGACATTTATTGAAATAAAGATGAAAATATTGCAAAATTTGCAATAAACTTCAACAATAATTTTAAAACTTATCATCCTTGCACTCTCCATGTTATCAACAATGCTCCAGATGACGATTTTAATTGACCAGAAGCAGGCTTAACTGTAAAAGTGAATGTTGATATGGAAAATAAAAAAGACCCTGTTAAAATGGCTCAAATTAAAAATGAGGCTATTAGACAAGTTAGAGAAAAAATAATAGAAGAATTTAAAAAGAGAGCTCCAAAGCTTGAAATTGATAAGTTATATACTATTGATTTTACTGATTCATTTTTCAATAATTTAGATTTGTCTATCGGAACAACCTTAACTTTAAAAGGCAAGCATTTTATGTTGAAAAATCCTTATACAGTTAATGTTATAAATACTCATTCATCAGCAAACAAAAAATTAAATTTAAAAGATTTAAATTTTGAAGTTATTACTTTAAGTGAAAAGAATTTAAATGATTTCAAAAAAGCTTTAAATGAAAAAATTATTGATATTCTCAAAAAAGAAAATTATCCTGCTAAAGAAATAATCAAATTAATTGAATTTAAATTATTAGAAAACCAAAAGTGAGATTCTTTCTTTAGTAAAGTTGGCAATAATGAATTAAAAATGATGATTCAATTAAAAGAAGAATCGAATTTAATAAGTGGAGATAAAGAATTTAAATTAATTAATATTTTTACAGAAGAAATGTTAAAAGAAGCTTTAGAAATTAAATCTAAAAACAAGAAAAAATTACTTTCATGGCTAATTCCAGTAATTATTTTAAGTGTTGCTGGAATTATTGCAACAGTTATTGCAATATGATATAGAAAGAAGAAGAAACATTTATAGATTAAGCCTATAGGCTTCTTTTTCTATTTCTGTGATTTTACTAAAAATACTATAAAACAGGCTTATAATAAAAATATGAATTTACAAGATAAGCAAAATTATTATGAAAGTAATTTGAATTTTTCATTAAGTTCATTAATTATGAATGATGAAAATTCATTAAATAAATTCATTGCTATTAACTTAATTAAAAATATGATTAACCAATTAATTAATTATAAATATAGTGATGAAAGAAAAAGTAAATATAAAGCTCATGATGTGATTATAAGAATTGTGAGATTTGAAAATTTTACATTTAAATTGAAAGTAGAAAGATATAAAGATAAAACAACAAATAAGTTGCATACATTTTATCCTGATTATTTAAATTTTGGATCATCTATATGTGAAGATGAAAAAGAAAGAATTCAAAATTATGCTGTTAAAGATAGAATCACTTATTCCAGAATTGCGAGCAAATACAATGTAAGCAAAACTCAAATATATAAAATTGTAAAACAAATAAATTTAGATAAAATAAACAGTCTAAAAAACTACAAGAATGAATTAGTAAACAATAACATTTTTATAGGTATCGATGATACATTTATAAATTCAAATCAAGGAAGAACAAAACAAAAACTAAAAATAAGATTTTTAACCATATATCAATTGAAGAATAATAAAATAGTTAATTTGAATAAAATAATGAATATTTTGCCATCTTCTAAAATGATTTCTGGCAAATGTTTATCAAAGACAATTAAAGAAGCAATATCAAAAAATTATGGAGATTATAATAGCTTTAATGTTTATATTTTAGGGGATGGTGCTCATTGAATTCAAACACTAGCAAAACAACTAAAAGGCAATTTTATTTTGTGTAAATTTCATTTTTTAAGAAAATTTAATGATTTTATTTTCAAAAAGAGAAATCGTGGATTTAACAATTTTATAGATAGCATTCAAAATTTAAGTGGGTGAAAAATCGAAAAAGAAATATCAGAAGCTTACAACAGTAGAGATTATGGAAAGTTGTTAGATATATTAAATACAATTTATCTCTATTTTAGAGACTCTTTTGTTTTGAGCGATATTGATAGATACAATTTAAATAGTTTGATAAGATACACATCTAAACTTGAAAGTGGAATCATTAATAATGATTATAGTTCACTAATAGAAGGACACATATCTGTTTATATTAAAAGAAAACTAAACAAAAGCTTTGCATTATATTCTCAAAAAACAATAGAAGCTTTATTGTTGAATGATAATAAAATTCAAAATACTCTTATTCAGACATATTAGTATTTATATTAATATTAGTATAGAAAGGCAATTAAATTTGCTATTTTTTCCATACAATTTTCTTTGTAATTTACATTTTTTAATATAAAATATAGTTGATAGAAAAAGGAAATACCGTTTTCTCTTTTAAATTTCTAACTTATCCCATTAATAATTTACATCACCAAATTCCTAATAAAAATCCATGAAATTTAAATTTGTATTTATGGAATCTTATTTGAAAACATAAATTAAATGTTAAATCTATAACTCAGGATAATGGATTAGAATTTTCAACATTATTTTTCATTGGATATAAACTAAAAATTTTTATATATAAAGCTGATCCATATGCTTCTTTTCAAAGGGGTTCAAATGAAAATTTCAATGGTTTAGTTAGAAGATTTTTTAAGAAAAAAACTAATTTTGATGATATTACTGAAGAAGAAATTCTAAAAGTACAAAATGAAATAAACAATCGTCCAAGAGAAATTTTTGGATATTTATCTGCTTTGGAAATGTATGAAAAATTGAATGCAGAAAATCCTTGAAATTCAACAGGTATTGACAAATTATTATATGGAAAACAAAAGAGAAACTGAGAAAGTAACAAGAATAGAAATTTGTTTTTCAAAAAAATAGGAAAATTAAAATAATATTTTAGAAAGAGCGACAAGTTGCCAAATATTTTAGGGAGGGTTAATTCCCTCACTTTTATTTGTCCTAGCGGACAAAAGATAATAACAATATTAATAATTAAAAAGGAATATCGACATGCGATATTCCAAATTTCTAGGTATAAACTCATAAATTACAATGTAGCAACATCCCGAAAGTTACGGGATGTTTTTGTAAAAACTATTTTATATTTTTTAATTGTTCTATAGTGTTAAATGACATATCTTGATAATTTTTGATTTCTAATATTTGAGCTTTTATATCTGTTTCATTAAAAACATCTAAAAATTCCATTTCATTTCCATGTTTATGAACATAATTTCCATCCCTACCTATTATTTTATTTTGAAAATCTAAAAGATATTTTAATATTTCCTCACAATTAAAATAAAAAATACTTAAATCATGAAAACCAGATGATGCATTTTCTCCAAATTTAGAAAATAAAACATCAATTTTATTATCTTGTTTTTGAAATATTTGAACGTAGGGTTTTTCTTCAAATACACATGGAACAATACCAACTTTTTTATAATTTTCTACAACTTTTTTATAAATTAAGTCTTCAATTAAACAATCTCCTCATAATATAAAACAAGTATCATTTGAAGAAAAACCATATTTTTGTTTGATTTCCAATAATGCTTTTAGTACAGCATCTCCACTACCCTTACCTGATGAGATAGCAATATGATTATATTTTTCAAAAAAATGCTCATTTTGGGAAGAACATATCGTAAAAATATGATCACAATATTTTGACATTCGCAAAATATTTTCCTCAAGTAAAATTTTTCCATTTACATTAACCAAGGCTTTAGGAGTTGCGCTTTTAAATCTAGTTTGATTACCAGCAGATAGTATAATTCCGAATTTCATCTATCTCCTTAATTATTTATCATAATAGGTATCAATAACAGATTTAACCATTTTTTCTGGTTCTTGTTTACCCTTAATATTTCCCATATCTTTGACTATGAAATTATAGTTTGGTATGGTTAATTTTAAGGTTTCTTTTGCACTTTTATTAAGTAAAATTTTTACATTTGATAAGTCAAGACCGGTTCTTTCCATATAATGGATAAAATCTAAATTAGTAACTCCAAATGAATCACCATCATCTTCATTATTAATGATTCAAATTTTTTGACATTTTGCCTTATTGATATAATCATAAAAATTCATATATTCAATTGTAGGTTGAATTGACGATCAAAAAGTACCAGTAGATATAATAATTAAATCCGAATTATCAATAAGTTCAATGGCTTTAGGATTTAAACCATATTTTATTTTTCCTTCATATATGGTGGAAATTATTCTATCGTTTTTATTGTTTCAAAAGACAGTTTTTCCTTCATCATCTATTATTGTTCCAGATTCCGTTAATGCCTTGATAAAGATATTATCAAATGAATTTAAAACAACGAAATCATCAATTCCCATTTTATCGCAAAATGTTTATTTGTTTTTTCATAACCTAATTCTTTATACATTTGAGAATATACTATATTTGAAACACTAAAGTCTTTATAATTAAATTCATTGGCCTTAGGGTTTTTGAAAAAGTTAGAAACATATTTTTTATATTCTTTTAAATTTCATTTATCTAAAAGTTTAGAAATTTCTAATTCTTCTTTATTTTTAGTGAAATCAAATCTTTTTTCATAAAACTCTAAAAGGTTTTTATCCACTTTATCGCCATATTCACTCACATACATTCTTGAGTGATTTTTTCTTATATCTGATACTCCTAAAGTATCAGTAACTTTTCTACATACACCAGTAGATTTTCCATTATCATAAGCATTTACAATAACTTTAATGCTTAAATTTTTATCTTTGTAAAAAGCTTTTAATCCTTTTACTAGTGCATCATTACCTGATCCACCTGAAATAATAGTTATTTTCATAAATAAATTTCTCCTATCAATGTTTTTAATACTAAATGTTTTTGTTGTGTTCAATTTGTGTTCAATTAAATTTATTGATATTTTTAAATATAGCAACAATTTGAAGAGGAAAGTATAACGCTAAAAACAAAGGTCAAATAAATAATGATATTATCATTTTTCACCATGGAACACGCTGAACTCTTTTTCTACCTCTTGCGTATGTAATTATACCAATTACAAATAATATTAAGTATATTATTGCATGACTTATAGAGAAAGATATTGCTCAATCAATTCACATTTGAGTAGCATTAGCTCCAAAAATAGGTCCAAGTGAAACTAACAAAATATTTAAAAGTAAAATTAAAAAACCTATAAAACTAATTGGTGATATCATAAATAAAATATCAATAGAAGAAGACTTTGATATTTTTTTATCATCCTCTTTTTTAGATGAAAATATATTTTTTATCAAAGATTTAAATCTTTTTCTTCAAACAATTCTATTTCCAAAAACTCATCTCAATCTTTGACGTCACATATCTTTAAATTTAGTAGGTTGTTCATCATAAAACATTGCTTCATCACAATATTTAACATTTTTTCCTTCTGAAATTTGTTCAACCGTAAAGTCTGAATCATCGGATGGAATAGTTATATTTCAACCATCTTTAACCATTTCATTGCTAATTAAAAAACCAGAACCCAAAATTTTTGAAGACATCTTAAACTTCATTCTAGCGTTTGATTCAATGGTTGTATGGAACATAAACATAAGTCCATAATTAATTGTTTGAATGCTTTTACCAAAATTTTTAGTATTTCTAAATGAAGTAATTACATTTTTTTTATCATAGTGTAAAAAAGCATCATTCATTTTTTCAAAATATAGACTATCAACTATATTGTCCGCATCAAATATATGATATCCATCAAAACTTTGTATACCATAGTCTTGTTCTATGAATTCAAATATTTTTTTTAATGCATAACCCTTAGTTCTTTCGCTAGAATTAGAATATTCATAAACAATAGCACCAGCATTTCTTGCTTTTTCCGCTGTTTTATCTGTACAATTATGTGCTATCACAAATGTTTGGAGTTTATCTTGATCATATTGAGAATTTTTTATGCTTTTTACTAACTCAGCAATAACTTTTTCTTCATTTCTAGCACAAATGACTATACCATATTTTAGCTTTTGAGTTTGAGTCTTAAATTTCACTGGCTTAGCAAATAAACCAAAAAATAAAACAAATCAATATTGAAATGAAAAAATTAAAAAAATCGCTACAATAATTGGCATAACTATATAAACTACATTTTGATATACCATTACCACTCCTTACTACATAAAATCCAATTTTTTAATTGTTAAATTTACTACAAATTTCTAAAATAATATTTCAAAATATTGTTTTAAGTATATAAATGTAAATTGTATTTTATTATTTTTCTCTTTAATTATATTTAAAATATAATATAAATAATTCAAAAGATAATTTTCAAATTATATCAAAAATTAAGAAGTAAAATATTAATTTAATCTTATTAAAATTTTTATAAAAGTGAGACATACCATTATGGATTTTTAAAATAATTTGTCCGTTAAACACAAATTGCTAAAGATATTATAAAAGTTTTTTTGCTATTTTTGTTAATAAATAAAAATAATTCGAAATAAAGCAATAGGAAAAATAACTCAGCTGAGTTAGGTAGTTTTATTTTTTAAAATATTTAATTAAGCGTATAAATTTGTATAAAATGATGGGTATAAATGTTTGTTTTCTTGAATAAATTTTTCAAATTGATTTGCTGACATAGCAAATGGAAAATCATCTACTTCATGTCTTACAAAATTATTTATTTTTTCGTTAATTATTTCAATTCATTCATGTGTTAAATTATCAAAAGTAATTGATTTTGGTCTATATTTTCTTATCATTTTATTACAATTTTTAATTCTACCTTTTTGACTTGAATTGAAAGCATCGCAATGATATATTTGCTTAATAGTGTTGATCTGATCTAAAGTGTAATTTTCTGAACCATTGTCAATGGTTAAAGTATTAATATTTAATTCATGTTCATCAATCATATGTATTAACGCTTCTTTAGTTGAATCTGCATCTCGTTCGCATAATCGCATATAAAATTTCGTGTTGCTCTATTCATTAAAACAGCAATATGATATAAATCATCGCTTTTGCCATGTACTGTATCAAATTCAAAATCGAACATTGTTGTAATTTCTGGTCTTAAACTTATATGTAATCCGACTCTTCTTTTCTTTGGAATTGAAGGGTCTTTTTTATGCCTTTCTCCCTTTCCTACAGACTTATTGGGCAAAATTGAGATATTTATATCAAATATTAAAATGTTTATATTTTTGTATATTGTAGTGAAGTGTGGAAACTTAATTTTTAAGCATTTTTCTTTAGCTTCATCAATTAATGTTCGAACTGATTTTTGGCAAAAAGTTCTTAAAGAATTTTTTCTATTTTCATTGCATAATCTATCATATACATTTGAATTTTGATTTATATATTTAATTCAATCTTTGTGTTTATTTTTAATTTTTTGATTAACTATTATTCTTCTACAATTTCTGTATTTATTTAAAACTGAAATTTTATTTTTTATACTCAAAATTGTAACTAAGGGCTTATAAGCCCCACAATGTGGGCACGTCAAACCTTTAAAAAATTTATCGGTTTTGGAAATCATTTTTCTATCATCTGATAAGTCATCAAATAGTTTTTGAAATGTTTCGCGTTTTAAATTTAAAGATTTATGAATATTCTTAAAGTTATTTAATTCTTTAAACATTTCTAGTGCTTTAATAACTGTTTCAATTCTTACATTATTGGGATGTAATTTATTTCTATTTAACGAATTCATAAATGTTAAAAGATCGTATTTTTCGACAAAAACATGTATATATTCAGTTATAAATTCATTATTTTTATTTTTAATTCTTTTGATGCAATTTATATTATACAAGGACAAAACAAAAAAAGTGAGATTCACTAAAATGGTGGGTCTCGCTTTTATAACTTTCACACATTTTATAAATGTTCTTTTCTAAATTCAATCTCACTAGAAATTATTTCATCTAAATTAGTTTTTACTTCATAATTTAATTCTTTACATATTTTTTTATTTGAAGCTATTAATTTATCAGGGTCTCCTGCTCTTCTTAGACCATATTCAATATTTAATTTCTTGCTTAAATTTTTTTCAAAAGCATTTACAACTTCAAGATTTGAAAAGCCTTTTGAGCTACCAATGTTGTAATATAATGAAGTTTTTTCTTTAATCATTTTTTTTGCAGTTAATAAATGTAATTCTGCTAATTCATATACATATACATAATCTCTTATGCATGTCCCATCAGGTGTGTTATAGTCATTACCAAAAATTTTGAATTCGGGAATTAAACCAAAAGCATAATAACTAATAGCAGGTATAAGATGAGTCGGTGCAACACCATTTTTGGTTAAATAACCAATTCTTTTACTTTTTGAAGCACCGGCAACATTGAAGTATCTTAAAAAAGTATATTTGAAATCACTATTTGTATTAGCAAAGTCTTTGATAATTTGTTCTCCAAAATATTTGGTTCTACCATAAGGACTACATGGTTCTTTTGGGTCTTCTTCAAAAAAATAACCATCGTGTAATGAGTTATTACCATATACTGCTGCTGATGAAGAAAATACCAAATTATTAACATTATGCTTCTTCATTGTTTTTAAAACATTTACTAAGCCTTGAATATTGGTTTCATAATAGTCAAGTGGTTTTTGTACACTTTCACCAACTTTAATTAAACCTGCTAAATAAATTACTACATCTATTTTATTTTTTTTAAAAGTTAAATCTAATGTTTTTATATCTAAAATATCTCCATGAATGAATTTACTTTTTTCTTCAATAAATTCTTTAAATCCTGTTGAAAGATTATCATAAATAATTACTTTATTTTGATTTTCTTTATTTATAACTTCAGCTACATGGCTTCCGATATAACCTGCTCCACCAATTAATAAATAAGTCATTATTTCTCCTTTCTATTTTTTAACTTTTGGTATTTTATATAAAAATTTAGTAACAAAAACTGGTAAATCAGAAATTCTTCGATATTTGATTTTTACATCAAAACCATTATATATTTCTAAAATTGAAGGATAAATATCTTTGATGAATTTTTTAAATTCTTTTTTTAAATTTCGATTTTTTAAATTGATATAGTAAACACTAATCAAACTTAAATGTTCCATAAATAAGGAATTTACAGTTTTTTTAAGAATTTTAAAACTTACATCATTTTTTAAATTTGATACTATGTTTTCTAAATTAAATTTGTAGGCTTGGTTGATACATTCAATTTTATTGATATAGTTTTTATCAAATGAAGTTAATGAATTGGTTTTTCTATGATAAATTATAGAATAAATCAAAGTTCTTTTAAAATTCTTACATTTGGAAAAAATGTTTCCCATTTCTGCAATATCTTCATATTTTTTAACTTTGCTAAAATTAACATCAATATCATATAAAAATTTTTTGTTGAAGAAATGTCCTCAAGCAAAAGTTGTGGTATTCAAATAATAATGCATTGGTGAAGGATTGATTTTCAATGTTTTAACAATATTATTGATAGGTATTTTAATACCTAAATCATCAAATTTATATTTAAATTGTGTTGAAAGTATTTCTAAATTAGGCTTTAAATATTTAACACAACGTTCTATTATTTTAGGACTTAAAAATTCATCATCATCATCGATGAAAAAAATGAAATCTCCGGTAGAAGCTTCAATTAATTTGTTTCTTGAATATCCTGAACCAATATTAGTATCAGAAAAGATATATTTAACTCTAGAATCTTTAATGGTCAAATCTTCTAAGATTTTTCTTGTTTCATCATTAGAGGCATCATCATGGAATAAAATTTCTAAATTTTCATAACTTTGATTAAGAATAGTTTTAATAGTGGAATTAAAATATTTAGCACTATTATGCAAGGGAATTAAAATTGAAATTTTAGGTTGATATAAAGTTTTTTGCCTATTCATATTTTTCCTTTTAATATAATATATATTACTTATTTTACTAAACATTCAATAATTATCAATGAATATAAATAATGTAAATTATAAAAGTGAGATCTCTAAAACATGGGGTCTCACTTTTGGAATTAACAGTTTGCAAAAAAGTTCTATTTTTATTTATAATTTAATTCTTTTTGAACTATTCAATAAATGGTATTTAATTTTTGATAAAATGCAGCATGTCCACATTCATTTAATTGCACAATTTTATAACCATAATCTTCTTCTATTTTTTTAATTTCTTTTGGTGTCACAAATTCATCATTTTCACCATAAATAATTACACAACCATTTGGATTTGCAAATAAAGGCTTTACATGTTTTAATAAATAATCTTTATTTAAAATTTGTTCATTTAATAGATAAGAAAAAGTTTCTTTGTTTTCATTATATTTGCTCGCTAATTTTTCAGCATTATAATCCAAGTTTCTTAAATAATAATCTTCAGGATTATAAACCAATGCTTTTAATGAATTTCTAGCATCTTCAACATTAGCAGGAATCAATCATTTTTTAAGATTAATTAGTTTTTGCTTATCGTCAACTAAAGCATAATTTAAAGGAGCTACCATAAATAACCTAGTTACGTTTTTGCATTCTTTTTTAGCATACAAACAAGGTACTGAACCCATTGAATGCGAAATTAAAATTACTTCGTGGTCTTTTAAAACTGTGTTAATAAATTCAACTACTATTTTGTTATATAAATCAAGTGTTATTTGACTATTTTTTCCAGATTTTCCACAACCAGGCAAATCTAAAGCATATAAATTGAAACGATGAAAGTTTCTATATTCTTCTCTTAATTCGTCATTATCGGGTGTATTTTCGGCCACTACTTCAGCAAAATCAATAAATCTAGTAAAGGTATTTGAATCAGAATTAAAACCGTGCACAAGTAAAACAGTTGCTGGTCTTTTTTCTTTTTGAAAACAATCTATTCTTTCATAATAAATTGTTTCGCCTAAAAGTTCAATTGATTGTCTTTTATACATTTTAGCCTCCTAACCAATTAACAAGCTAACAATTTCTTCCTTAGTAACAGTTCCAAAATATTCATTCAAGTTTTTAATTTGATTTAAAACTTCTAGAATGCTTTGTTCATTTAAAGCTACATTACTAAATAAGGGTTCAATTTCATGAATATCTTTTTTGCTTAAAAAGTCACCTTCAAAAACTAATTGTTTAATAGTACCTTTTTCAATATTACCTTTAACTACTAAAATTCCACCAGGAAATTTAGCTCCATTTTTATATTTAAAAGTTGCTGCTTTATTATATATTCATTCTTCAGAAGCAAATTTTTGTTGTAAAGAGTTTAATTGTTCTTGATATTTTGCATAATCAATATCAATAAATTCTCCTTTAAAATTCTTAACAAAATAATTAATTAGACGACTAATAAATTCTTCAGCTGTCATTTTGTTTAATAATTCATCATAAATATTAACTACTCTAGATCTAACTGATTGAATGCCTTTAGATTCATACTTAATTCTTGCAGGATTAAGTGCTTTCGATAATTTTGTTAAATCAACATCAAATAATAATGTTCCATGGCTTACTATTCGATTATTACTAATATATTGGGCGTTACCACTTATTTTGCAGCCATTAACCACAACATCATTGCGACCTTTAAATTGAGCATCTAGACCTAAGCTTTTAAGAAAAGCAATAATTGGAGTTAGAAATCTTTCATAACCGCCTTTTTTATCATAGTCAGTTATGAAACTAAAATTAATATTGCCTAAATCATGATACACAGCTCCACCGCCAGACATTCTACGAGCTAATTTAATATTATTTTCTTTTACAAAAGAACGGTTAATTTCTTCATATGCATTTTGATTGTTACCAATAATAATAGCATTATCATGTTGATAAATTAAAAGTACATCACCTTTTAAATCATGATCATGCATAATAATATTTTCTAAAGCTAAAGTAACATAAGGTGAAGTTTCCTTAATTCTAAAAATTTTCATATTAACCCCTTTACTATATGAATATATAAATTTAACTTTATTTTATACAAAAAAGAATTTTTGTAAAGTAAAAGCATAAATTAATATCTTCAAAATTTTAAAGAAAATTGTACCAACCATTTTGTATATTTTTTTCTTGACTTAATCCCAATAATGGTATATAATGATGTCGATGTGTCCCCTTTAGATGGACAATTACTATACCCATAGGGATAGTTGCTTAAGAGTATATAGACTCCTTTCTTTAGATAATTGAAAAGTGCTGTGCGCGGATGCTTTTCTCATTTTATTTCAAAATATTATATTAATCTTCAATGTAAAAAAAGGGCTCAAAAAAGTCCTTTTTTTATTTAAAATTTTTGCTTTATGTGAAAGTTTTTTTTACCTCTCTAAAAATTTTTTCATTTTATAATCAAATTTCAATTACATTAACCTCTTTTTTAATAATTGATAAATTTATCTATTTTATAGGCTTTTTAGTAAAATATTTTTTTAAAGTTTTTCAATTTTTTAAAAAGATTATGATTAAAAAATTAGTATGGAATAAATTCCTTATTTTTTTCACATAGTTAATTTTGAAAATTATTTTTATTGATTTTCTTTTTATAATTTAAGAACCTTAATATTTATTATTAATATTAGATTTTAGGGTAATTTTATTAAATGTATATGAAAGGAGAAAAATGACTACAGCACAAACTACAGGTATAGAAAACAAAAAATCTAAACCAAAAGACAATAGCCGTAAGGGAAATTTTCGTAAGATTCTAAGTAAAATCTCTGGTGCTTTCATGTTACCAATTTCCGTTATGTCTATAGCTGGTCTATTTCTAGGGGTTGGTGCAACAATATCAAGTCAATCAGCGATTGTTCTTAACAACAATCCATTATCATCTGCTGCTAAAGCTGGTATTGTAATTGGTGACTTTATTAAGTTATTAGGTGACCCAATATTTGGAGCAATGCCGCTATTGTTTGCCGCTGCCTTTGTTGTGGCATTTACCGACGAAGCCGGAGTCGGTGTCTTCGCCGCAATAGTTGGATACTTAGTATTCTCAGCAATACAAGCTCCATTTATTCAAAGTGTTATAGAAAAAACTAATATTGATGGCACTAAACAAGCTAGTGAATTAATAGGTAAAACAATACTTAAAGATGATAAAACAGGACAACTTTACTATGAAACATTAAAAGGATATAACATTCTTTGAATGACAGGTAAATTTGGTGCTGAAGTTAAACAAGTCGTTGGTTCTTCATTAGGATTCAAAACTCTTCAAACTTCAGTTTTCGGTGGTATTTTTGTTGGTTTAATAGTTTCATACTTATACAACAAATTACACACAATTAAACTTCCAACTGTTATTTCATTCTTTGGAGGAAAACGTTTTGTTTCATTAATAACAATAGTTGCTATGATTCCATTAGCATTCTTATTCCTCCTTATATGACCATGAGTTGGTATTGGTCTTAGTTACTTTGGTAATAGTCTTGGCCATGTACCTTATGGAATTGAATCATTTGTTTTTGGTTATATTGAAAGATCATTAATTCCATTTGGTTTACACCACGCATTTTATGCACCTCTTTGATACACAAATGCAGGCGGTGATATTCTTAAAGCGCTTGAAGATTGAGAAGCAAGAGGAAATACTTTTTCAGGAGATGCTACTGCATTAACTGCGTACAATGGTTGAAAAGCTTTGATGGCTTCTAAACACAATGAATGAATGGGTGACTCATATGCTGCTATTGGTGTAATGAAATTAAATTTCAACACTATAACATGAAAAGATGCTACACCAGCACATGAAGTTCATACATTACCTTTATATAAATTTGTTGCTAGTGAATTAGGCATTAAATTAGGTAGATTCATGCAAGGCAAATACTCATTCATGATTCTAGGATTACCGGGTGCAGCTGTTGCTATGATTTTAGCGGCTCCTAAAGAAAATAGAAAAGTGGCAGCTGGTACAGTTATTCCTGCAGCTCTTACTTCAGTTATTACTGGTGTTACAGAACCTATTGAATTTACATTCTTATTCTTAGCACCATGATTGTTTTGGGGATTCCATGCATTCTTCTGTGCATTATCATTCTTGTTAGCTAACGTTCTTGGCGTTCACATTGGTATGTCATTCTCAGGTGGAATTATGGACTTAATCATCTATGGCATTATTCCTGTGATGAAAGGTACAAACTTCTGATGAGCATTAGCAGTCGGAGTTCCTTACTTCTTTATTTATCTTGGTGGTTTTTACTTCTTCATCAAGAAATTCAACTTGGCAACCCCAGGTCGTGGTGAAACAGTTAGATTATTCACCAAAAAAGACTTCCAAGCTAAACAAGATGGAAATGATGAAAATTCTTCAAGTAAAGATACTTCAGCAAGAAACGCAAGAGAATATGCAATGATTCTTGCTTTAGGCGGTCCAAAAAACATCGTTAACACAAATAACTGTGCTTCAAGATTAAGATATGATATTAACGATAGAAACTTAGTAAATGAAGTTGAATGTAAGGCAGCAGGTGCAGTTGCACTTAAATGAGAAGGTGATAAACACGTTCAAGTTATTGTTGGCCCTGTTGCTGAACAAATGAATGCTAACATTAGAAAAATTTTAGCTTCAGGTGAATTAGATGACATTGTTGTTGAAGCAAAAAGCGAACCAACACAACCAGTTGTAGTAGTTGAAGCTCCAACACAAGAACAACCTACTGTTCAAGAAACCCCAGCAGTTACACCTGAACCTCAAGTTGAAACATCATCAATGACAGAAACTAAATGAGTTGACGAATCTAACGACTTAGAACGTCCAGTTGAATTCTTTGCTCCAGCAGTTGGAGATGTAATTCCTCTTAGTCAAGTACCTGACGAAACATTTGCAAATAAATACTTAGGTGATGGTATTGCAATTCGTCTTAAAGCTGAAAAGAAAGCTTCAATTTGAGCTCCAGTTTCAGGTACTCTTGAAACAGTATTCCCTACAAAACATGCTTATGGTATTACAACAGAAGAAGGTATTAAAGTACTTATTCACATTGGTGTTGATACTGTTGCTTTAAATGGCGAAGGTTTCGAAACCAAATTAAAACAAGGCAAAAAAGTTAAAGCTGGTGACTTGTTATGTACAGTAGATGTTGAATACTTATCAAACCAAAATAAAGTAAGTGATGTTATCGTTGTAATTCTTAATGAATCAGAACACAAACACATTATTGATACAGCTCTTGGCGAAAAAATGACTAAACGTGCTGACAAATTAATGCTTGTTAAATAATTTAACTTACAAAGTGGACTCGAATTTGCGAGTTCATTTTTTATTATTTCAAAAAAGAGTAGAATAAAAATATAGATAAAAGGAGGACTAACATGAAAAAGAAAATACTTTTATCAAACTTAATTTGAAGTTTACCATTATTACCTTTAAGCACATTAAGTTGTAATAATCAAACTTCAAACAACAACTCCAAAGATTTTGAAATAGTTAACAATGAACTAATATCTTTTAACAAAAATAAAAACTACGCGGGTGAAATTACTTTACCAGAAGGTATAACTAAAATTAAAAATGAAGTATTTAATGGTTTTGATAAAATAACTAAAATTCATTTACCTCAATCTTTACAAACTATTGGAAACAAAGTTTTTGCCTATTGTGTTTCATTAACTGAAATTACATTCCCCGCAAATTTGATTAAATTAGGTGATGGTGTTTTTTATGGTTGTAGAAGTCTAACAAAAATTATCTTCAACAGTCAACTTCAAATAATTGGAAAAGAAACTTTTAGCCATTGTAATTCAATAGAAGAAATAAACATTCCAGATACTGTAAATTTAAACACTGAAGGTGCTTTTGAAATGTGTTTTAATTTAAAAACCGTTCATATCCCAAATAACACAACAATAATTAAAACTAATGCCTTTAAACAGTGCGAATCATTAGAAACATTAGACTTAACAAATACCAACGTGAATCAAATACAAGCAAATACATTTAAAGGATGTACAAACCTAAAAGAAGTTAAGGTCAAAAAAGACACTCAAATAGACCCTCAAGCAAGCGATAATAATTCAATTTTTGTTTTTAATATTTAAACAAAAATTGAATGCAATTTTTACTTATTACATTTTTTTATAGGCTAATTAGAACAAAATAATATCAAAAAATGAACCCTTGTTTTTAAGCAATGGTATGAACCCCAAAAATTGGACTTTTTAATTATGTTTGGACTTATTCCTGTATTGGACAGGACTAAGTCCTTTTAATTTATTAACAATTCTGTCATTATTATAATATGAAATATATTCTCCTAAAGCAGTTTTAAATTCTTTAAAACTATTAAATTTCTTTTCTTCTCCAAATCAAAATTCTCTTTTTATAACACTAAATAAACATTCAGTAGGACTATTGTCTAAACAATTTCCCTTTCTTGACATGCTTTGAGTTGTTTGTTTTTCTTTCAAATAATCAATATAATCTTGATGAGTGTATTGTCATCCTTGATCAGAATGTAATAATACATTTTTTAAGCTGTGGCCATTCTCTTCTACGTTTTCTAACATTTTACCAACCATTCTTAAATTAGGCGATTTAGAAATCGAATAACCTAAAATCTCTCTGTTTTCAAAATCAACTACAGGAGAAAAATATGCTTTTTCATCATTTTTTAATTTAAATTCAGTAACATCTGTTCCTAGTATTTTCAAATATTTATTATTTGAAAAATTTCTTTTGTATCTGAAGAAATCTTCTGTGATATTTTCTTTATCTAAAAGCAAGTTTGGAATGTTTTTGTGAGCATCACCTTTGAATGAAGAATATTTATTTTTTCTTCTTAGCGTTTTGCCGAAAATATTCATTGCTTTCATTAATTTTTGAACTTTTTTATGATTGATTATTATTCCTTTATTTCTTAAAGCAAAAGTAATACGTCTATAACCGTATAGTCCTTTATTATAGTTAAATAAGTCAAAAATAACCTTTTTTAAATTTTCATCTTTATCAGGTTTATTTTGTGATTTTAATATCTCATAAAAAGTTGACTTTTTCAATTTAATTTTTTCTAATATTAATTTAATAAAAAATAGCTTTTTAAATTCTTGCCTTAGTTCAATTGTTATTTGGAATTTTTCTTTGTTAGTGAGTCTTTCAATTGAACCAAGGCGCTCACTTTTTTTGCTATCGCATTCTCCAATTTGAGTATTTTATTTTCTTCCTTTAATTGTTTAATTAATAAATCTTTATTTTCTATTTTATTTTCAATATTGATTTTAAAATTTGTTGCCTTTTGAGCATCTAAATTTATTTTAGATTTTCTTCCTTTTTTCTTTTCCATAGCAAAAGAATTATAACCAAACTTTTTGTATTTTTCTATTACTGAATAGCATGCTGAATAAGAGTTTTCCATTATTTTAGATATTTCTAAAATACTAAATTCTTGAAAATATAATTCGATAATTTTTATCTTTTGATCAAGTTTTAATTTTAAATAACCTTTTATATCTCTATTTCATTTGTTTCTTTTTACTTTTTTAAATTTAAATTGCATAAAAATGAACCCCTTTCCAAAAAGTCCGGAATTTGGGGTTCACTCTACAAAAGTTCATTTTTTTATTATTATTCTGTTTGTTCTTTTTCAACAACTTCAATATTGAAGAAATACTTTCTTCAAGTTAATCTCAATTTTTGATTTTTAGTTACTTTTTTCTCATCAAAATATTCTAAGTATAAATCATCAAAGAAATGCATTGGACAGGCAAACGATCTACCTTTACTAATTAATTTTAGTGATAAATCATCTAACACTACTTCAACATTTTCACTAATATGAAACTTGAAATAATTGTCTTTATTTTTAACCGCTGAAGGATTAATTGAGATACCTACTTTATGTACTCGTTGTAACTCATTGAATGCTTTAAATCTTAGATCACCATTGAAATTAAAATACCCTGGATTATATTTAATTTCTAAATGTCTCATTAATTCTTCAAGAGTAGCAAATGGTCTTGAAGCTCATAACCTTCTACTGAATGTAAAAAGTGAAGGACAATATCAGTATCTATTTTCGATTATGTTAACTTTATAAACTTTAATTTTAGTCATTTACTAACTCTAATTCTTTAATAATTTTGGCAATTCCATCATTATTATTTGTATCTGTTACATAAGTTGCAACTTTCTTTAAATTCTCTGTTGCATTACCCATTGCAACTGAAATTTTAACAGATTTTAACATACTTATGTCATTATCAGCATCACCAAAAGCCATTGCTTTTGATAAATCAATATTTTTAATCTTAGCTATTTTTTTTAATGTTTCACCTTTTGATATTCCTATTGGCATTATGTCAATAACACCTTTTTGAGAAGATACCGTATATATTTGTTTTGGAAATTTGCTTGCAATTGTAAGTAAGGAGTCTATCTTCTCTTGTGGAACGTTTTGAGTGATGATTAATATTTTAGCAAAGTTAAATTGGTTACAAGTTTCTTTTAAGCTTCCTTCTTGATAATCTCAACGATAAGGATTAGTTTCCACTACATTCTTATAGATCATAAATTCGAATCAATAAGGGTTCGAGAAATTATTACCTTTCATACCATTAATATCGTAAGCTAAAAAATCAATTCTATTCTCTTCTAAAAAGTTAATTAATTCTTGAACAACTTTTTTGTCTAGTCCTTGAAAATAAGGCACTTCTTTAGTGATAGGATCATAAATCATAGCCCCATTAACTGCTATTATTGGTAGTTTAATATCTAAATCCTTAATTAATTTATAGTTCATATAATAAGGTCTACCAGTAGCAATTATTATATTTTTATTTTCCTTTTGAAGCTGTTTTAAAGTGTTTTTGGTTGATTCTAAGATGTTTTTATTATCATCATAGAGTGTACCATCTAAATCAAAAATGAAGTAGTCTATATTATTTATTTTGACCATAATAAGCATTCTTTCCGTGCTTACGGTTATAGTGCTTTTCAATCAATGCTTCTTGCGCAGTTGGAGCACTATTATTTGAAACTATTAAGGTATTAATAGCCATTTTTGCCACTTGTTCAAGTGTTAAGGCTAAGTCAACAGCATCTTTAGGAGATTTGAATGATCAAGCAAAAGGCCCATGTTCTTTAACTAATGTTGCGGAACAAGCTTTTCAGTCAATCTTGCGTTTTTTAAATGTTTCTATTATTACAAGTCCTGTATTGTGTTCATATTCTCCATCAATTTCTTTTGGAGTTAGAGCTCTAGCACATGGCACTGGTCCATAAAAATTATCTGCATGTGTTGTACCAAAGCAAGGAATATCTTTACCTGCTTGTGCAAAAGCTACTGCATGAGGACTGTGAGTATGAACAATACCTTTTATTTCAGGATTTTCTTTATAAAGTAAGGTATGAGTAGGTGTGTCTGAAGAAGGGCGATATTTAGAATCAAATACATTGTTATCTAAATCAGTTATGACCATGTCATCAGCTTTAAGTGTGTCATAAGAAACACCACTTGGTTTAATAACCATATATTTACGGTCTTGTGTTATACCTGATACATTACCTCAAGTATGAATAACAAGTCCATATTTAACTAATAACATATTAGCTTCATAAACTTCTTGTTTAAGTTTTTTGATTTCAGATTCATATTTTTTATCAATTGCCATTATTTGTCACCTCCTACCTTAGCTCACATTTTGTCATAAAAATCATGAGCATCTTTAATGTATTTTATAGCTTGTGTTTTTGTTTCATTTTCTTTATTTAAAGATCACATTTCTATCATAATAGGTTGGTTCATTTTTAAATCTTTTAATATTTTTAAAAGATAAGGGAAATCTACAGTGCCAGTTCCAAAAGGTGTATCTCTAAATTTACCAGGTACAGTATCTTTAAAGTGGAAAGCTACTATATGATTTCTAGCAAGTTCAAATTCGTTATACAAATCTTCTTTTTTACTTCATTGATAAATATTACCTAAATCAGGATAGATTTGTAAATATGGACTATTAATCATGTTGATATAAGTCATTGCTCTTGAATTAGTTCCCATGAATTGAGTATCCATAATTTCAAAAGCTAACATAACTGAATATTTACTTGCTTCAGTAACTGCTCATTTCATTCCTTCAATAAAGTTTTTTCTTGTTTCTTCATCACCTGGTTCATAATAAACATCATAACCAGCAAGTTGAACAGTTCTAATACCTAATTTTTTGGCTAAAATAATTGCTTTAGTCATTATTTCTTTGGCTCTAGCTCTGATTTTAGGATCTTTAGAACCAAAAGGATATTTACGATGACCACTAAGGGTCATTGAGTTAAAGTTAAATTTGTGTTTGATTAGTAAGCTTTGAGCATGAGTAATTTCTTCATCACTTCAGTCTAAGCGAGCTAATCTTTGAGGACTTTCATCAACTGAAAATTCCATAAAGTCATAACCAGCTTCTTTAGCAATTATAATTTTTTCTTCTAAGCTAAATTTGTTGTTTATAGCTTTTTCATAGATGCCTAGAAGTCTTTTTTTATTTTCAATATTTGGCAAATTCGTCCTTAAAGGCTTTAGCAGCTAATTCAGGATTTTCAGCATCTCTTAATGATCTTCCAGCTATAAAGATGTAAATAGGAATATCTTTAAATAGTTTAATATCTTCAAGAGCAACACCACCTGTAATAGTTACTTTAAAACCTTTTTTAGCAAGTTTTGCAACAGCATCTATGTCTTTTTGTCCTCATTTAACTCCACTAGCTTGACTGTCTCTTGAACGGTGTCAAACAACTTGAGGAACTTTTACTTTTGCTCAATCATCAACTTGATCTCAAGTAAAGTTTGAAGTTAATTCAATTTGAACTTCTTTATTTTTGCCATATTCTTTGGCTATATTCATTGTTTCAACAATTGTAGGCACTTCAGCTGCACAAATGCATGTTGTAAAGTCAGCACCATTTTCGAAAAACATTTTTCCAAAAACTTTCCCAGCATCTGCAATTTTGCCATCAGCAACAATGATTTTTGTAGGAAATGCTTCTCTCAAAGCTTTAATAGCTTTTTTACCTTCTGAAGCAATAAGAATAGTTCCTACTTCAATAATGTCTATGTATTTTTCAACCTTTTTGGCACTTGCAATTGCATCTTCAATTGTTAAGTTATCTAAAGCAATTTGTAACATTGGTTTCATATTAAGTCCTAACTAAGGTATTTAGTATAATCAACACCTTTTATGATATTTATAATTTCTTCTTTTGTTTTAGCTTGTGCTATTTTTTCAATATTTTGTGGATCTTCAAAAACAGCAATAATTTGTGGAATAGCAACAGATGTATGAGTTTCACCATCTTTTGCGGCTAGTCCCATAAGGATTTTTACTTCTCTTTCATCTCCTTCAAATTTGATAGGTTTTTTAAGAGTAATAAGTGAGAATCCGTTTCCAAAAACAGCGTCTTTTGTTGCTGAAGCATGTGGCATAGCAAGACCATCTACTAAAATGAAATATGGTCCATGTTTTTTAATTGACTCTAAAATGCAGTCATAATACTTAGAATTAATAACTTTTGCTTTTTCTAATGGTAAGCAAGCTTTCTTAATTGCGTCTTTTCAATCTTTAACATCAACATGAGGTACGATTGAATCATTAACAATTAAGTTATCTAATAAATTTAATTTATCAGCCATTATTACATCTTGCTTGATGCATCTTTTAGAGCAGCTTTGATTTCATTAACATCCATGATGTTTTTTAAACCATAAGCTTTTGCTTTGCCTTTTGATACTTCTGAAACAAAGTTTGAGGTACACAAAACAACATCAAAGTTGTTTACAACTGTTTTGCCTTGACCTAAACCTAAAGCTTCAACTGTTCCTGGAATTGAAAGTTCTTTCATAGCTTGTTGAACTTTCATTTTAATAATCATACTTGTGCCCATACCTGAGCCACAAACACATAATACTTTCATGTTAATCTCCTAAATTTTAATAATTAGTATATATAATTTATATTAATATAAGCAATAAAAGCAGAAAATTCTGCCTTTATTTTTACTTTTTTGCCTAATTAAGCAGCTTGTTCTGCAACTTCTGGTTGAGGAACTTCTTCAACTGGTGCATTAGCAACTTCTTCAGCTTTCTTTTGATCTTCTAAAGTTTCGACTTTAACTTTAAGCATTCTTTGGAATACTGTAGGTTTGTGTTGTCTGTGTGAGTCAAAGAATTGAGCAGCAAAGATCATACCAACAACGTAAAGAGGGAATACGATGTAAGCTGCATATGGGTGTCATCCACCAAGAATCATAATTGAACCAAAGATACATGTTCAGTCAAACATACCATTAAATCCTGTTAAGAATGGTGTTCTTTGGAATGAAGATAATTTCAAGATTTCTTTCATATCTTTAGCTTTAGCTATTTGGTCTGAAACATATCTTGATAAGTATTGCATATCTGGGAGAGATATAGCTTTAGCATTTTTACCATGTCCTAAAGTTCAGTTTGTTGTAACTTCACTTAAGCTTTTTAAGATTTTAAATGAATCTTGACTGTTAATGTTTGTAATAGCATCATTTAATTGTGCTGCTGAAACAGCTTTAGTAGCATCACCTTGAATAAACAAGTTAGCAGAATTTGCATTTAATGAATCTGTAAATGATTTGAATGCATCTGCTTTTGTGAGAGTTTTTTCAAAGTATTGGAAGTCAAGATCTACTTGGTGCATTACACCAAAGTTTTTAATCATACTTAAAGCAAGAGCAACAACAATAATTTCAACGAAACCAAAGATCATTGGTACTGCAATAGTAGCTTTATATCCACCTGAAGCATTTGCAAATACTCCAATTGAACCTGAGTTAAAGAACAATGTAATGAATAATGGAACTACCATAACAATTTCAAATCTTGAGTTTGGAATTTGAGCTAATCCAATTACAAGACCCATAGCTAAGAATTGGCCAATTGAACCAAATAAGAAACCATAGGTAACTGAGTTTTGGCTGAAACCATATGTGGCAGCAACGTCAACAGCAACAACTGATCCTGGAACTAATTTTTCACTAATTCCTTGAAAAGCTTGTTGAAGTTCTGAAACGAACATACGAATACCAGCTTGCAATGTAAGGATAGCAGCAATTAATTGTAATGACCCTAAAACAAGGTTAATTGGTCAATAAACTGCTCCACCGCCTGGTGTTCATGAACCATAAATTGCTTTGTTTACAAGTCCATTGCTTAAACCAAATCTTACTGATGCACCTGCAGGTGCGAATTGAATAATTAAGATAAGGATTAAGAAAATTAATAAGATCAATACACTTTGTGTGAAGATATTGTCTTCTAATAATTTAACTTTGTTTGAAAGTTTTTTAGTTTCTGCTGAATCTTCTTTTTTACCGAAGAATTTACCAATTTGGTATGCAATAGCAATACCGAACATTTGTTGGTGACCAACACAGAAACCAGCTTTTTGTGTAACAGCATCTGAACCTTTAATTGTTGAAGATGAAGCTACACCTCAGTAAGTACCAAGTAATAAACCTGAGAAGAAGATTGTACCGACTTGGCCGCCTACATTAATAGCACCTTCTGCTGTTCTAAATTGTTGTCCTGAGAACATTAATACAAGTACAACTGGAACAACAACAGCTGCTTGTTGGAACATAACGTGTCCTGTAATCATAAGTGAGTGAACATTTGTTCATCTTCTTAATGCAATTAAAATAATGTTAATTGTTAAACCAATTAATAGGGCATATGCTAATCATGAAGCAAAACCTTGACCAACTGAGTTAAGGAAGTTTTCTACTGAAACTTGTCCTAAGTATGTATCAAGTGGTGTAAGCCCTTTACCGAATTTAGAGAATGCAAGGAACACTGGTTTAGCTAAACCAACAATCATTCCTGAACCGATTTGCATCATTAAGACACCAATCATGGCTTTAAGCATTCCAATAAGTGAGTCTGAAAAGCCTCTACCTAGTATTAAATAGCCAATGAAGACAATTAATCCGATTAATACAGCGTTAATTCCTAAGAAGTTATTTAATAATAAGTCTTTTGCAAAGAATAATGTACCAGCTTTAAAATCTCATTTTTTGAGACCTAAAGCAAGAACATAGAATCCTGCAAAAATAGCGATAACTACAAGAGCACCAACTAAAGCTTTTCAGTTAAAAGATCTTTTTGTCTTGTTTTCTGACATAAGATATCCTTCCCCCGAAAAGTGGAATTATTTTGAGTTAAATAAGTATATAAGTTATAAGTAATTTTATGTTTTTAAGTTTTTATGGATAAAAATAAATGAATAATTAATTAAGTTAAGTTTAATTTTAATAAATATATAAAAGTTTAAATTAATCTAGTTTTAGATTTTTTTTAAGTTGTTTTAATTCGTTTGATACTTTTGATGGTTCATATTTACGAGGTTCATCAAATGCAAGAATTTCACGTGGGTTGTTGATCAAAATGTCATCAATAGCATCTTGAGGAACACCAACTTTTTTAAGTAAAGGAATAAATCTATCAAATAAGTATGAGAATCCAAATGTTTCTTTACCTTTTGTTAAACCATAGTTACGTTGGTATAGAATTCTACCAGCATCAAGGCTTAATGTTATATGTTTTTGAAGACCTTGGTCTACTAAGTATTTAATGTTATCTGCAAGAGTTGAGTCAGGGTAGTATTTAACTCTGTCTGGTCCATCAAGACAAAGTGTAACGCCTGTATCTTTAATAACTTTTTCATAGTAGTATGGGTCTGGGTTTTTATTTAAGTGAGAAATTTGAATTTTACGAGGGTTTGCACCAAAGTCAATTAAGTGTTGTGCAACTTCTAAAGCCATTGTACCTAATTGAGTATGTACTAAAATTGGGCAACCTGTTGTAATTGAGGTTCTAGCAGCAACTTCAAGTGCTTTTAATTCAAGACGGTCAATAGCAGCATAACCTGTACCAGCTTTAATAATACCAGCTTTAGCTTTACCACGTTTTACAACTGGTCCATTATAGTTGTAAACGTCCATACCTTCTTCAATTTCTGCGACACACATTTTAACAATGTCGTCTGTTGGAACATTAGCTAATCATGAACAGTATTTGTCATAGAATTTAGCTTTGTGGAAACCTGTAGACATAATAATGTTTGCTTTGCCTTTGAATGCATTAGCAATTTCCATCATTCTAAATACATCACGTCCAACATTAGGTGGATCCATTGTAACCATTGTTTTTCCACCGTGATCAACGAATTCTTGAAGTTCTTTTTTAGCAGCTTCAACATCGATCATTAAAAAGTCAATGTGTTCTTCCATTTCAGGGCCACCGTTCTTTATAAGGTGGTCGTGACAGTCAGTAATACCGATAGAACTAGCAGGCACATCGCCTAGCACAGTCCTAACAAATTTTTCTTTCTTCATAAATCTCCTTTTTTGTAATCAATAGAACCCTTTTTGATTTTTGGTTCTAAACTTATTTTAGATAATAAATTTAATAATTAAAATAAATAAAATTTGCGTATTTTGAAAGTTTTTTCCAAAATTTTTATATGAAAATCTTTACAGCTTTTTTTACTTTTCTTGATACCTTATAATATATATAAGCAAAAATACATAAATAAATTGAAAATTATTTTCAAATTTTTAAAATTTTTAAATCTCTAAAAATTAACAAAATTAAAAAGATTGTAAAATGTGAAAAATTCTCATTTTTTAAAACTAATTTATTTAAAAACCAAAAAATATATTTTCATTCAAAACACACTTAAACTTAAAGCATTTATAAGAAAATAAATGTATAAAAAAATTGACAAGCAAAATAAAAATCTTAGCTTATTGAGCCTAAAGTTTCTACTTTTTTGCATTATTATATTTATCAATATCATCCATTACTAATTTATATAAACCGTTAGGACAAAAATTACCTTTTAATTAATTTCTTGTTAAACTATTGAAATAAGTTGAAAGATCATTGTCTACAACTTTCATTAACTCTACAACATGTCTTAATTCAGTTGCCACATCATGTTTTTTACTATATTCAACAATTGTAACAATGTTAGGCAATATTTGTCTAAATGTGACTTTCTCATTTGTCTAATTTGGTCATAAGTTTCTTTTGCTCTAACATTTGCTAACTCAAAACTTTTAATATTGTTTAAAAGATTATTTGGGCCATTTGCTAAAATATTTTTTATCTTGAGCAACTTTTTTAATAGATTCATTAGCAAGATTTTATATTGAAACTATTTCAGTCTCATATTTTGGATATGAATCTTTGACAATGCTATATTTAACTAATTTATTAGTTCATTTTTCGATTTGTTGTTTATTTTCTAAATATGTATCTTTAGCTGGTTTTATTGTTTTTCTCTTTCTTTTGATTATGTCTAGCAACTAAAGCAATTAACAATGCGCCTTTAGCTGTTGCTGTAGTAGTTGTTAATCCTTATAAAACTTTAAAACCTTTTATTAGATTTATCCTGACATGATTCAATTCATTGGTCAATTTTAATCTTTTATTGCATATTTAAATTATGTTTAAAAATGCTTAATAATGTTATTTTTGTATTTTTTAAATGATAAAAATCACTTGAAAATACATATTTTTTATAAATTTTTCACACAATAATTCATTGATTATTTTATTAATAAGCCTATAAAACAGTTGTTTTGGTAAAAGAGATGTATTTTTAACTTTTGCTTTTTATGAAAAAAGATAAAAAAATACCCTTTTATCTAGGGCATTAAATAATATTATTATTTTTTGATTCTTTTTAGTGTGGGAAATAAAAGTACATCTCTAATAGAATCTGTTTCAGTTAATAACATAACTAAACGGTCAATTCCAATTCCACAACCACCTGTTGGAGGCATGCCATATTCTAAAGCATCAACAAAGTCATAGTCAATATCACTTGCTTCATCATTACCTTTATTCTTTTCTTCTAATTGACTTTCGAATCTTTCTAATTGGTCAATTGGATCACTTAATTCTGTATACATGTTGGCATATTCTTTTGTGTTAATAAATAATTCAGCACGTTCAGTAAATCTTGGATCATCACTCTTAGCACTTAAAGGAGAAATTTCAATTGGATGTCCATATAAGAATGTAGGTTGAATTAATGTTGGTTCAACTAATTCTTCATATAAAGCATTAATAATGTGTCCAATAGTAAAGAATTTTTCGATTTTGATTTTGTAAGCTTTAGCTACTTCAATAGCTTCTTCTAAAGTAATTTTTCTAAAGTTTTTACCAGTTTTCTTGCTTACTTCATCAATCATATTTACACGCTTAAATGGTTTTGATAAATCAACTTCAACACCATTTGTTACAAATTTACTTTTACCAATTTTTTTGCAAAGTTCTTTAATTAATGTTTCAGTTCTATTCATCATACCTTCAACATTTGAATAAGCTTCATAAAATTCAATAGTTGTAAATTCAGGGTTATGTGTTGTGTCGTATCCTTCATTTCTGAATATGCGACCCATTTCATAAACTCTTTCAAATCCACCAACAACTAATTTTTTAAGCGGAATTTCTGTTGCTATTCGTAAAACAAAATCTTGGTTCAATGAATTATGATGTGTAGTAAATGGTTTAGCAGCTGCTCCTGAAAGATAATCATGCAAAAATGGAGTCTCAGCTTCTAAATAATCAAGTTTATTAAAGTAATCTCTAATTCATTGAACAATTTTTGTTCTCATAATGAATGTATTTCTTGATTCTGGATTAGTAATTAAATCTAAGTATCTGTGACGATATCTTTCTTCAGTATCAGTTAATCCATGAAATTTATCTGGAAGGGGTTTAAGAGCTTTAGTTAATAATTGAATATCTTGAGCCCTAACTGTCATTTCACCTGTATGAGTTTTCATTAATAGACCTCTAACTCATAAAATGTCCCCTAAGTCAAATGTTTCAACTATTTTAGTTAATTCAGGATTTTCTTTTTTATTGAAATAAACTTGAATTGTTCCTTTACGATCTTTTAATACTAAGAAAGGTCCTCTAGCTGTAATTAATCTACCACTAATATTTTTCTTAACTTTTTTAGCTTCTAATTCTTCTCTAGCAAATTGACCATATTTTTTATTAAGTTCATCACTTGTACTCAATTTACCTAGACCATAAGCTTTTTTAAAAGGTACTACATCAAATTTTTTATAAAATTCTAGTTTATCACGACGAATTTGTTCTTGTTCTGTGTATTTTGTCATGTTTTTCTCCTTATTATTAATATTAAAATTAATTATAAAGTAAATAATTAAAGAATTTAATTAATTTTTAATATGAAAGTTATAAAAGTGAAATTCACTAAAATGGTGGTCTCACTTTTATAACTTTAAATTCATTTTAGATTTTCTTCCTTTTTTCTTTTCCATAGCAAAAGAATTATAACCAAACTTTTTGTATTTCTCTATCACTGAATAGCATGTTGAATAAGAGTTATTCATTATTTTAGATATTTCTAAAATACTAAATTCTCGAAGATATAATTCGACAATTTTTATCTTTTGATCAAGCATTAATTTTAAATAACCTTTTATTTCTCTATTTCATTTGTTTTTTTTCTTTTTTAAATTTGAATTGCATAAAAATGAATCACTTTCCAAAAAGTCCGGAATTTGGGGTTCACTATACGATGCTTAGTGGAGAACCAAGTAAAATTAGTTTCTTCATTCTAATAATTCCTTTTCTATTCTATTTATTATCACGATGAAGTTATATAAAAATGTGATTTTTTTAAAAAATTTCAATATTTTTTTACACAAAAAATTACAAATTTATATATAAATTTTTAACCAATATCATTGTTTGATAGTTGTTTTGGTAAAATTAACTATTTCTTTAAAACTAGACCCTATCATTTTTTTAAAACAAAAAATAATAAAAAAAATAATATTGTGGTAATATATAAATGCAAAAATAAATGTTTTGTAAATAGCGTGGTCGCGTAGCTCAGTAGGATAGAGCACGAGCCTTCTAAGCCCGTGGTCAGAGGTTCGAATCCTCTCGTGATCGCCATTTTTTTATTTTTATTTTTTGTTATATAATACAAATATGTCCTTGTAGCTCAGATGGATCAGAGCACGAGCCTCCTAAGCTTGGGGTGGGTGGTTCAATTCCACTCAAGGACGCCATTTTTTTATTTATGTGGTACTTTTAATTTTTATATATAAAAAATAGTATAATTATTTAGTAAAAATAATGAGGAGAAAATCTATGAATAAAAAATTTTTAAAATTAGGCTCAATTGCAGGAATTTTATCTTTTGCACCAGTAGCAATTAGTGCTGGATGTGGTTCTAAAGGTGGAGAAACCCCTACTGATCCAGTTGCTGCTAAGAAAGCATTAGTTGAACAAGCATTAAAAGATTTAAATGCTAAAATTGAAACTGTTACTGATGAAACTAAAAAAGCTGAACTTAAAAAGGAAGCAGAAGCTATTAAAAAAGATTTCGATGCTGCTAAAACAGTTAAAGATTTTGAAGCTGTAGATGCAAAAATTAAAAAAGTTGTTGCTAAGGTTGAAAGTAAAAGTACATCTGACCAAGACAAATATAAAACATGCTTTACTTTAGGAGAAGGCAAAGATAGAATTAAAGAAGCTATTGAAGCCATAAAAAAAGATTCTAAATCATATTCATTTGCATATGATCGTAAAGGCCACAACATTGTTTGCTTTAAAGGAAGAATTAATTGAAGCAATGGAGACAAGGTTATTGCATTAAATATAACAGGTGCACTAAAAGAACATATTGAAAAAAATAGTGTACAACTTGCAAATGCAAAAAAACCTACATATAATAGCAAAAAAGGAGAATCTATTTCTTCAATTCTTGATTTTAAACTAGAAGGCAATACTGTAAAATTCAGCTTTAAATTAGCTAAATTTGAAAACAATAAACCTGTTTTGGATGATCCAATTGTTTACAATGCAGAAGTTTCTTTAGCTTAAAAAATAATTAAAAAAACTAGCTCAATAAATAAGCTTAGCTTACTAAAAAGGGGCTAGTTTTAATTTTTCATATTTGACAATAATCTAATTAAATAAAGTACACAATAAATATAATCATATGCTAATTTGAAACCAAAGAAAAGAGATAATTTTATCATTTCTTTTTTGGTTTCTTCATCATCCATAAAGTTGTTATAAACTTTATTGTATTTGCTAATAAAGTATCAATCAAATCCCATGTAAAGTGCCATTAATAAAACACCTAATGCTGAAATTAAAGCAACTATATAACTTGAAAATACAAATCATGATACAATACCTAAAATCAACATTGTTACTACTACCACTATAATAGGAATTCAAATTTTACTTAAATCAACTTTATTAGTTGCTGCTAAAGTTCCCATAATAAACATAAAACCTGCTGGCAAGAATAATACTAAATATAATTTTCATCAAACCATGGTTTCTTTTGCATAGTAAAGAGGAATAGAAAGCGCAAATAGACCAAATCCCATCATTATGATCATAACCATAGGTATGGATACACCTATATGCATTCTAGGTCCTAAAAACATATATACAAAAAGTAAAATTATTGTTAGAATACCTCCAATTATTCATCCAATAACATTATTTGCAAAATAGCCAGCAATTTTTTCCATATACAATGTACATACAAATGCTGTACCGAAACTAATTGCCATGCAAAATGCAAATCAAAGTAATGTGTAACCTAAAAAACTATTTCTTCTGACAAAGACATCGTTAGTTGTTTTAAAGCTATCGTCTTTTATTTGTTCATTGTTCATAATCATTATTCATAACATATATATTATAAAGTAAAAAAATAAAAATAAAAAAATCGCCTACGCAGCGATATGGTTGCCCCAGTTAGATTCGAACTAACGCATGACGGAACCAAAACCCGTTGCCTTACCGCTTGGCTATGGGGCAAAATGGTGGAGGGGGAGGGATTCGAACCCCCGAACCGTGAGGAAGTGGGTTACAGCCACCCGCGTTTGGCCGCTTCGCTACCCCTCCATTTGCATAAGCAATGTAATTATAATATAAAAACATTTTTTGCATATTTAATTTTAATAGAAAAAATTAATTTTGTATGCAATATTTTTTAAAATTGTTTTTGCGTTTTACCAAAAGTCCTGTTTTATAGTTAAATTTGTCAAAAATTACCTAATAAAATTTTTTACACCACTTTTTTTAATATTAATAATATTAATCTAAAATATATATTATAATTAAATTATAATTTAACTCACAAAATTGATTATAAATGCTTATTTTATAGTAAAAAAGACAAAATAGGAGGAAATATGTTTGGTTCACTAACTGAAAGAAACATAAAAGATATCATCAAAAAAGAAAAATTAACAGATGTTAAATATTTAATTAAATATATTGAAAAAAATCCAAAACTTATGGATGTTAGATCAATGTACCAAGTAGCTCAATATGCAAACTGCTTGAGATTAGGTCAAAAACCTTTTTATACAGATGAAAGTTTACTAAACTCAATTTACAAAAGACTTCCAAATATTAGAGGCAAAGAATTAACGATTGTTGATCCTTTATGTGGTGTTGGTATTTTCTTACCTTTAATTATTGAAAAATATGGAGACAAAGACAAACTAAGTATTACTTTTTATGATGTAGATAATGAAACAATAAATATTGCTCGCGCTTTATCTAAAATGATAAAACTTAAAGTTACTTTTGGTGTTAGTTTTCAAAATGCTGATTTTCTAATGAAAAGAACTAATGAACCAATTGACTTATTAATTGGTGATTTCCCTAACATAAGAATGTCTCATCCAGCTTTAAGAAAAAAATATCCTTCATTAACTAAAGAAAACAAATTATTACAAAGTTTTGCTCCATTATACTTAATGCAAAGTAAAAAATGAGCCAAATATATTGTTACAGTTTTAACTAAAAACTTTTTATCAACAAATGAATATGAAGAATTAAGAAATGACATTTCAAAATTTACAATTGATCACATTGTAGATTTAGATACTCTTGGTTACAACGGAATTAGTAATGAAAACATTATTATTTTTGCTAATTTAGCTAAAAAAGTTTCAATGAGCAATACAACTGTTGTATATTCAGCTAAATTTGAAAAATTGCATGAAATTAATCAAAGATTCTTAACTTCAAAAACATATCCTAACTGAATACTTTACCGTAATACTTTCTTTAACTCAATAGCTCGTAGAATGAATTTCAATTCATTAAATATCATGAAAAATTCAATTCCTCAAACAACCTACAATGAAAGTAACTCGGTTTGATTAATTCAACCATTAAATTTAAATAACAAAAACAAATATGTTAAAAACAATAATGGCACTGACTTATTTGTTAGTCTAAATTTAATTAAACCAAATACTAATTTAGCTAAAACTTTAGAAAGAAAAGACCTTTATATTCTTTATATGAACACAAACCAAATTTCGGTTGTTCCTAAACCTGCTAATGTTGCAGTTAGTGACAAACTATACATGCTTGAGCCTTTAACTCAAATTACTGAAAAGGATTTAGAATTCTTTAACTCAAAAGATTTTAGACACTTTTATCAAATAGCCACAAACTTTTCATCAAGATCAAGCTCAGTTGATACATCAAGTGTATTCTACTTCGGTTTGCTTCAAGAAGATGATGTTGAAACAAACAAAAAACAAAGCGAAACTCAAGAATACGATTTCTCAGATGACTTTGATATTGACACCAAAGAATACGAAAGCAGTTTCTAATACAATTTTTACTTATTTCTATCCTAGCATTGCTAGGATTTTTTGTTATTTTTAAAAAAATCAAATAATAATAATTTATTATCAATATAAATTTTAAATTTAATATATAATTTTTTTGCAATTTGTAAGTAACAAAAGCGGGAGAGTATAAACTTGTCTATTCTATTCTTCTGGTTATTAATTTAGCCTAAACTTTAGAAAGACTAACAAATGCAAAAAATAAATAAGGAGATCAAATGAACAAAAAAGAATTCGACAGTAAAGAATACCTTGAAAAAGTTGATGCTTGATGAAGAGCTGCAAACTATCTTTCAGTTGGTCAAATTTACTTAAGAAATAATCCTCTTTTAAAACATCCTTTAACTTCTGATGATGTTAAAGTTTACCCAATTGGTCACTGAGGAACTATTTCTGGACAAAACTTTGCTTATGCACACTTAAACAGAGTTATTAACAAATATGACTTAAACATGTTCTACATTGAAGGACCAGGACATGGTGGCCAAGTTATGACATCTAACTCATACTTAGATGGAAGTTACACAGAATTATTCCCACATGTAACACAAGATGAAGCCGGAATGCAACATTTATTTAAATACTTCTCATTCCCTGGTGGAACAGCTTCACATGCTGCTCCTGAAACCCCTGGATCAATTCATGAAGGTGGAGAATTAGGTTACTCAATTTCACACGCAACAGGTGCAATTTTAGATAACCCTGATGTTATTGCTGCTACTATCGTTGGTGATGGTGAAGCTGAAACAGGACCTCTTGCAACTAGCTGATTCTCAAACTCATTTATTAACCCAGTAAATGATGGTGCTGTATTACCTATTCTTCACTTAAACGGTGGAAAAATTTCAAACCCTACAATTTTAAGCCGTAAAAGTAATGAAGAACTACAACAATACTTTAGAGGTATGGGCTGAGAACCTCACTTTGTTGAAGGCGACAAACCTGAAGTTATGCATGAATTAATGGCAAAAACTTTAGACTCAGTTATTGAAGAAATTCAAAGCATTCAAACAAAAGCTCGTAAAAAACCAGCTGACAAAGCTAAAAGACCAGTATGACCAATGATCGTTCTTAGAACACCTAAAGGTTGAACAGGTCCTAAATCATGAAACAAAGAAGCTATTGAAGGAAGCTTCCGTGCACACCAAGTTCCACTTCCAATTAATGCAGAAAACATGGAACATGCAGATGCACTTGAAAAATGATTAAGATCATATCGTCCTGAAGAATTATTTGATAAAAAAGGTAAATTGGTAAAAGAAATCGCTGCTATTGCTCCTAAAGGCAAAAGAAGAATGGGCATGAACCCTATTACAAACGGTGGTATTAATCCTAAAGTTATGAAACTTGGAGACTGAAGAAAATTTGCTCTTCACTTTGATAGACCAGGTTCAGTTGTTGCTCAAGACATGGTTGAATTAGGAACATACTTTGCTGACTTAGTAAAAAGAAATCCAGAAAACTTTAGAATTTTTGGACCAGATGAAACTAAATCAAACCGTCTTTACAACTTATTCAAAGTTACAAACCGTCAATGAATGGAAAGAATTGATTCTAAATTAGATGAAGCTCTTAGTCCAGTAGGTAGAATCATTGACTCACAACTTTCAGAACACCAAGCTCAAGGTTTCTTAGAAGGTTATGTATTAACAGGTCGTCATGGTATCTTTGCTTCATATGAATCATTCTTAAGAGTTGTTGACTCAATGGTTACACAACACATGAAATGATTAAGAAAAGCAAAAGAAATTAACTGAAGAAAAGATTATCCATCATTAAACATTATGGCTACTTCAACTGCTTTCCAACAAGACCACAATGGTTATACACACCAAGACCCAGGTATTATTGGACACATGGCAGATAAAAGACCAGAACTAATTAGAGAATACTTACCAGCTGATACAAACACATTATTAGCCGTTATGGATAAAGCATTTACAGAAAGAAATGTAATTAACTTAATCGTTTCTTCAAAACAACCAAGACACCAATTCTACTCAGTAGAAGAAGCTGAAACATTGGTTGAAAAAGGTTTAGACATTATTGATTGAGCTTCAACATGTTCAAGAAATGAAACTCCAGATCTTGTTGTTGTTGCTAGTGGTACAGAACCTAACTTAGAAGCTTTAGCTACTATCTCAATTCTTAACAAAGAATATCCAAGTATGAAAATTAGATTTGTTAACGTTGTAGATTTACTTAAATTAAGACATCCAAAAATTGACCCACGCGGTTTAAGTGATGAAGAATTTGATGAAATCTTTACAAAAGACAAACCAGTATTATTTGCTTTCCATGGTTTTGAAGGTATTTTAAGAGACATCTTCTTTGACCGTCACAACCACAATTTAATTGCTCACGGTTATAGAGAAAATGGTGACATTACAACATCATTCGACATTCGTCAATTAAGTCACATGGACAGATTCCACATGGCTAGCGATGCTGCTGCAGCTGTATTCGGATCATCAAAAGCAAAAGAATTCATGGACAAAATGGAAGAAACAATCCAATTCCACAACAAATACATTCGTGAAGTTGGTACAGATATTCCTGAAGTTAAAAACTGAAAATGAGAAGGTCTTATTAAATAATTTAGACCACAAAATGGACTCAATAGAGTTCATTTTTTTAAATTATTTTTTTCATATCTTAAACAATTTTATTCGAGGGAAAAATGAAAAAATAGTATCAGAAAAATTACCAAAAACACTATAAAACAGCAAAATAATAAAAATAAAAGTACTTCGCTAAGTACTTAAATTAATTATATGTCCCATGTTGATGCAATGCTAATATCAAGGCACAAACCACAAGTCCAGCCATAAATGTAATCATAACTTTTTTGAACAAATCTTTGTCATGATGTGCATGATAAAACTCAGGTAGAAATTCCATCACAGCTGTAAAGAAGAATATACCAGCTATCATTGCTTGTAAGAATGCTCTTAGTTGTCAAATTTGTTGAATATAATCCCCTGTGAATATTCCTAAAAGCATCATAGGCAAGAATAAGAATAAACAACCTATTGATATTCCAATTGCTTTTCAACGACCTACACCCATTTCACGTTGTCTATAATAAAAAACCATTTCTTCAGGTATTAAGTGCATTATAAATGAAACAAAGAATGCAATACTTAATGAGCCAATATTCTGATTATATAAGTTATTTAATGAATAACCAATTAAGAAACCTTCTGGTATTCTGTGAGTTAATAACAACAATAATGCAACTACTTTTAGTTTGTTATTAGCATTTTTTTCGTCTGTTGATATTTTATGTTCTTGTGCCACATGATCATGAGAATGTTCTTCATTTTCACCATGTTCATGGTTATGAACCATATCATGTGTATGCACAAATACACTTGCTTTACTTTTAGCTAAATGTTTTTCACTTATCTTTTTAACTGTCATTCTAATTCCAAGTGCAAATAATAATCCACCAACTAAACCTGAAACTAATAAGAATATGTTTCAACCATATGTTGCTCATTTAATCTGCGCTGACGAAGCTTCTGGTGGCATTGATGCCGGAGCACTAATTGAACTTATTTCCAAAGCTTCTTTTAAAAATCCAAATAAAGCCATCGTTAAAAAGAAACCAGTTACAAAAGCATAAAGCAATATACTTCCTTTTTTACTTAAGCTTTTCTTAAATAAAGGAAAAATTAATGCCACTAAAACTGGCACTAAAAATATAATTGCAAATGCAATTAAGATCAAATAGAATTTAGCTGCTATTTCTCTTGTGCCTATTGTTAAAGCACCATTTTCAAGAGCTCTAATAAATTTTCTCATACTAACTTTCTACTTGAAGTTAAAGATAATAATTATTCTTCAAATTCAATATTGTGAAATACTTCTTGAACATCATCGTCATCTTCTAAACGAGCGATAAATTCTTGAAGTTTTTCACTTTTTTCTTTATCAAAAGCAACTGTTGTGTTAGGAATATATGTAACTTCACATTGCATAAAATCACTAATATTTAAGTTACTTTCAACAGCGCTTTTACATTTTGAAAAGTTTTCTGGTGCTGTTGTAATTATATAAGAATCTTCAGTTACTTCAAAATCTTCGGCACCATTTTCAAGTGCAACCATAGTTAGATCGTCTTCTCCAACTAAGTTTTTTGAAACTTCTAAAACACCTTTATGATCGAAAGCAAAAGGTACTTGACCTGTTTTACCTAAAGCTGCATTTTGTCTATTAAAGTGTGCTTTCATAGAACTTGTTGTTCTATTCATATTATCTGTTAAGGCAACAACTAAAAATGTAGCTCCCCCTGCAATTGTTGCATTGAATAAAACTTCTGTATAAGCTGATGCATCTTTACCATCACCTTTTGCTTTTGCTAAAGCTCTTTCAATATTTGCTTTAGGCATGTTTTTACTTTTAGCTTTTGCAATAGCTAATTTAAGTGCTGGGTTCATATCTGGATCTGGTCCACCAGCTGTTGTTGCTGCTACATAAATTTCTTTAAACATTTTTTGGAATATCTTACCACGGGCAGCATCGTTTGCTCCCTTCTTGTGTTTAATTCCTGCTCAATGTGAGTGTCCTGACATAATAAATCTCCTTGTGATTTTTTTATTTTAATATTGTATCAATTTCTTGAGGTTGTAATGAAACATTTCTTTTGTGTTCACTATTTTTGTACATTTTGTCTATTTTATTTTTGACCTCTTGACTAATTTTTGCATTAGAATCCATTAAATAAGAATCCAAATCTTTATAACTGAATCCTAATTCATCTTCATCATTTTGTCCTTCTCAAAGACCGGCAGAAGGTTTTTTATTAATAATTGAGTCTGGCACTTTTAAGTGCTTTGCAATAATTTTTACTTCACTTTTTAACAGTTTACTTAAAGGCAAAATGTCACTTCCGCCATCACCATATTTTGTAAAATAACCAATATGAGTTTCACATTTATTACCTGTTCCTGCAACTAAATAATTGTTTTCTTGAGCTAAAGCATATATGACAGCCATACGCAATCTTGGTTTAATATTACTTAAAGCTAAATTGCTGTTAATTAACGGTAAAGCCTGCTTTATAGTATCATAGGTATTTTTTAGATCAATAGTTATAAATTTTAAATCAACTGCTTTTTCTAATTCTTTGATATCTTTTAAATCATTTTGCATGCTATCAACAGGCATTATTACACCTAGAACATTTTTGCCAAAAGCTTTTTTTGCTAAGGCGACAACTACAGCAGAATCTATACCTCCACTAATACCTAAAACAATTCCTTTAGCACAAGCATCAGCAACTCTCTCTTTCATTCAAGAAATTAAATAGTCAATATAAGCTTTAATTTTTTTATCATCAAAATCTGCTTCATATTTAGCTTCTTTTCTTAATCCAATATGTTTTTTCATTAATCCTCTTTTTTTTAATTATTATATTTTATATAAAAATATTTTTAGCCAAAATTTTTTATTTTTATGAGTGCTCCATTTTCAAACATTTTTTAAATATGTAGGCATAGATGAAACATTTTTTGTATTCAAAAGTCAAAAAATGTTTAATATAAACCAACATCAAGTTTTTCATAAATATTTTCTTTTAAAAATTAATTTTAATTATTAAAATCAAAAAGGGATAATAAGAAGGTAAATGAATAATACTTAATTAAAAGAAGAAAAATAAACTCTTCTTTACAATTCAAAAATTGTTTTCGTTTGAGAAATTATATCGACAACATTGCTAAGTATATTAACTATTATATTTTTATCAATATGTGCAAATTTATTCCTATGTTTATAAAAACTATTGATCATTGAGATTTAAAAACAAATGCAACAACTAGAATAACAAAATTCGCTAACAAATATCAAGATGAAAGAATAATTTTAGGTATTTTATCAATTATTTTTTCATTTATTTTTGTTTTTCTTGGCGGATAGAATGGAAAACTTAAATATTGACATAATATTTGTGGGAGTTTCATTAGGTTTGATTTTTAAATTTGTTATAGATATTATGCTTATTGCAGCTTAAGGTTATTTAATTTATTTATTAAAAGTATTATGAAAAAAGAATACAAATCAACTGCTTTTATAACAACAGCAATCCAATTTGATTTATCAATTCTCTTCACAATATTATTTGTAACATTTACATTTAAACCTAAAAGAACTTGAAATGAATATTCTAAGTATTTAATAACCATGACTATTTTTGCATTAATATCTATTGTTTTATGTAATTTTATTTACACGTGAAATAATATTAAATTGAAAAAATCTTTACCTGAAAACAACAAAAACGAATTCCAAAAAATTAATGAAACAATTTAAAACTATTAAGACTTAATCTACAAAAATAGAATAATCTTTTTTATACATTTTTGACTAATAATTCTATAAAACAGTCGTTTTTGAAAAAATGCTTATCAAAAAAGTAGCTTTTTGAGCTACTTAAAATGATCTGGTGCCAATTATGAGAATCGAACTCATGGCGGCTAATTACGAGTCAGCTGCTTTACCACTAAGCGAAATTGGCACATATAAATTATTATAAATTATACAAAATAATAGTAAATAATACCTTTATTCATATTTTCATATCGATAACTTTTATTAATTTAAGCAATATAATTTTTTTGTGCAAAGAAGCACACTAAATTAAAAAATGGAGATATTATGAGTTGACAATTAATTGTTGGAGAACTAGTTGGTACTTTCTTATTAATACTGCTAGGTAACGGCGTTTGTGCAGCAAACAATTTTAAAAATATGCATAGTAAAGGTGCAGGTTGATTATTAATTGCAATAGGTTGAGGTTTAGCAATTTTTGTTGGTGCTTTAGCTGCTATTGGAATTGGTGGACTAGGTCACTTGAATCCTGCAGTAACATTATATTGATTAATTTCATTTGGAAAACACACAACAGGTGAGGCGTGAGGTAATGGTATTTTAATGATCATAATGCAATTTGTTGGTGCCTTACTTGCTCAAATTTTATTAAATGTAATGAACTGAAAACACATTAAAGAAAATGATTTAACCCTTCTTAAAGCTTCAAGTGCCACAGGTGCTTCACATGAAAACGCTTGAGTACAAAATGTTCTTTATGAATTTGTTGGCACAACTGTTTTAATTCTTATGATTTACTTAATAGAAAAATCAAAATTTACTATTTTAGACCCTCTACCTGTGGCATTAGTTATTATGTCTATTGGTATGTCACTTGGTGGTGTAACAGGTTTTGCACTTAATCCTGCAAGAGACTGAAGTCCAAGACTTGTTTATCAAATGACAGTATTATTCTTCTTTAAAGACGGAATAAAAGATGAAAAAACAGGAGAAGTAGTTAAAGCTCCTTCAGCAAATTGAGTATATGGTTCAATTGTTCCTGGTGCTACACCTTTTGCAGCAGGTTTACTAGTTGGCATTATTCCTTTAGTTGAAACATTTATGTAATCAAAATTAAAAAAGCGGTCTTTTCCGCTTTTTTTAATCAACTAAATAGTTTTTAATAGTATAGTTTGTTTCTTTCCTTTTCTAAAGAATGCATACTTTTTAGAAAAGTTTTTTGGTTTATAAACTGAGTTTTCTTCAACTATATCTTCATCTATTTTTAAAGCTTTAGTTGCCATAAATTCTCTAGCTTCACGTTTTGAAGCAATAAAACCTTTTTCAATTAAAGTTTCAATAATATTTTGATTTTGCTTAATTTCAACTACTGGTAAATATTGCTCAATTGATTTAAGATCTTTTAAATTTAGTTTGCTTGTATCAAAATTTTTGTTAAACAAAACTTCTGTAATTTGAATAGCTGAATCTAATTCATCTTGACCAAAAATATCTCTAACTACTTCACTAGCTACTACTCTTTGAGCTACATGTTTAAACGGTGCTTGATTATGTTCTTCCATAATTTTTGCTATTTTTTTATTATCTAAAAATGTTAATCAATTTAAAAATGTTTCAACTTTGCTATCTGGTTGATTTAATAAGTATTGATACATTTCATATGGTGAAGCCATAGTTTTATCTAATCAAAGTTTGCCACCACCTGTTGATTTACCAATTTTATTTCCATTTTCATCAGTTAATAAGTCTGCAGTAACTACAACAGCTTTATGATCATCACCCTCAACTTTAGAAATCATTTCAAGACCTGTAGTTAAATTACCTCATTGATCTGAACCACCAACTTGAATCATTATATTGTAATTTTTATAAAGTTGCAAGAAGTCTCAACCTTGTAAAACTTGATATGAAAATTCAGTAAATGACAAACCTCTTTCAATTCTTTTTTGAACTGAATCCTTAGTCATCATATATGAAACATTAACAAGTTTCCCGGCATCTCTTAAGAATTCAAGCACATTCATTTTTTCATAAAATTGAATGTTGTCAATTACTTCTAAGCCTTGAGCTTCTAATTGTTTTCTAATTTTACTTTTATTTAATTTCAAAGTTTTATTATCTAAAAGAACTCTTTCACTATCTTTAAATGAAGGGTCGCCAATCATTCCTGTCGCCCCTCCAATAAGTCCATAAACTTTAAAACCATATTGCTTAAATCTTTTTAAAACTGAAATTAAAACATAGTTTCCTAAATGCAAACTTTGCGCAGTTGGATCAAAACCACAATATACAGCAACTTTCTTAGGATCAAGTTTTTTAAACTTATCTTCATTACTAATTTGCTTTAATATTCCTCTTAATTTTAAATCTTCTAATATTGTCATGATTACTCCTATTTGCTTATTAATTCTGGCAATAATTCTTGTATTTCTTTGCCTAAATATTTTTGTTTTTCTTCTTCTAAAACATTATTGAAAAAGCCTTCTTCAAATTCTTTATAGCTTTTTGCTTCTAAATCTAAAGATTCTGCTGAGCAAAGCATTCCATCACTTTCTACATTCATAATTTTACCATCTAAAATTTCTAATCCATTAGCTGTAATTGATCCTGGTAAACAGAATGTAAAATATTTATTTTCAAGTGTATATGTTGTGTTAGTTATTATTTGTTTTACTTCATTTCCAAAATCTATTTTTAATACAAATAATCTATCGCTTTTTGGGTGTTTTTTTCTTTCTACAATTTTACCAATTCTAAAGTATTTAATTGATTTTAGTAAATATTTTTTGTTATATTTGTGCAATTTATTGACTAGTTTTTTAGTTTCATTTTCATTTAAAAAACCAAATATTTTTTCATCTCTTTTTTTACTTTTTGCATTCAGTAAATTAACCGAATTTACATTAAAATTTTCATCTCTAAAAACAACCATATCTTCATAAATATCCATAGTTTTTTTGTTTATTGTTGTATCAAAATATATGATTTTTGTGTTCTTAAATTTTGAATTTAAATTAAAAAATATACCCATAATATTTTTATTATATAATATAAAATATGAAATATGCAATAATTGTCGACTCATCATGTGCTTTAACAAAAAAACAAGCTGAAGCACTAGGATGATTTTATACCCCTCTACATATAAATATCGATGAGAAGGAATTTGAAGATGGTGTTGATATCAACACCTCTAATTTGTTTAACTTTTACACTAAAAAAGCTAAAGTAAAAACATCGGCTATCAATATAGCTAAAACTACAGAATTATTTGAAAAATTGTCAAAAGAGTATGACAAAATTATTTTCTATCCAATCTCTCATCATTTGTCAAGTACTTATCAAACTTCAAAAACAATGGAAGAAGAGTTTCCAAAATTAAGAACAATTAAATCGGTTCAAATTGTTCAATTAATTGTTTTAGATTTAATTTGATTCGAACAACAAATGGCTAAAGATCCTTCAAAAATGGATGAATATCTTAAATACATTGAAGACGGGGGGTTCCACAAATCAATTACCCTTATACCTAAATACAATGATTATTTAGTTAAAGGTGGAAGACTGCATCCTGCTGCCGCAGCCATAGCAAAATTATTTAAAATTGTTCCTTTAATTGCTGTTGATGGTGGTCAACTTAAAAAAGAAGGCGTCGGTCATGTTTTTTCAAAAACCACTATTAAAAATATAGTATCTAAAGCTAAAAACTTCAAAATCAATGACAAGAAAAATGTACTTCTTACTGCTTATATACACACAGGTGCTTCAGAAGAAGAACAAAAAACTTTTATTGAAGAATTCAAAAAACAATACCACCAAGACCCTTGAGTTAACTGACTTGCTCCTGTTGTTGCAATTCACACAGGTCCTGAAGCTTTTTCAAATGTTGCTGTTGAATGCCCGAAAGAAACAAAGCAAATATTCTGAGAAAAAATGGACAAAATTAAAGATTTATTATAATGCTTAAATTTCACGATTTAAGTTTTATTTTTCCATTTTTGCAGTAAATTTTGCTTTTTTACTTTTAAAAGTATAATTCTTGCATGAAAAACAAATCATTATATTGAGACGGATATTTTATGGCTTTAGCTAAAGTATCTGCATTAAGATCTAAAGATCCTTCAACAAAGGTCGGTGCTTGCGTAGTTAATGAAAACAAAAGAATTATTGCTCTAGGTTACAATGGAATGCCAAAAGGCATAGATGACGAATTCCCTTGAGACCGTGAAGGTGAAAGTCCAAAAGAAACTAAATATGCTTATGTTGTTCATGCCGAAATGAATGCAATTCTAAATACAAACAATAATCTTCAAGGTTGCACAATCTTCACTTCTTTATATCCATGCTCAAGTTGTGCTAAGACCATAGCTCAATCAGGCATTAGTGAAGTAGTTTATGAAGATGACAAATATCATGACACTGAAGACGCAGAAATAGCAAGGCACATTTTAAATGCAAGCAATATTAAAACAAGAAAAATTGAAATAGATACAAATGTTGAAGTTCAAGTGGGTAAAAACAAATACAAAAATTAACACATTAAGAGCTTATTTATTGGCTCTTTTTGTTTTGAATTAATTTTTAATTTAACTAATAATAAAATTATTAAAATAGCAAGTCAAATCAAAATAATACTTTATCTAGCTGATAACAAAGCAATCAAAGATAAATATGTAAATGAAGTAGCAATAGAAAAATTAAATTTAAACTTGATAATAATCCTTATCTAGATAAATTTATAAATCATTCCTAATTCATATGCTATGTCAAACAAGTTATACACAAAGCGTCCAATATTATGAAAAAGCAAATTTTGTTTATATTGTTTTTTCTCTTAAATATGTTTATAGTGATGGCGAAAAAGTATCATTAAAAAATATGACTATTAAAAAATACAAAAAAATCACAAATTATAAAAAAGAACAATTCATTAAAGATAACTTAGAATTGGCAGTTTCGGAGAAATTTTAAAAGATTATTTTCAAGGTTTTAAATTGGAAACAATGATAGTACAAATGTTATTGTAAAAATTAATTAGTATTTTTGAGGTATCAATTCTCACTCTAATTTTAGATCAAGTCATGAAATTTACAAAAGTATACAATGGTGGTTTTAAAAAAACACCAAAGATAAATTACCTGACTATTACACAGATGATCAAATCAAAGAAAAACTAAATGAATTAAATAGTAAACTTAAATTTGAAGTTAAAAATTTCAATTTTGAAAAGACTATGTTGAAAAATTAACTACAGAATGTTAAAATTATTTTGCCTTATGATTTTAACTAAAATACTATAAAACAGTCGATTTGGAAATTAAAAATGATTTACAAAGATTCTGTTGAGAATTACTTTACAATATATCTGGTGGCACCAAAATAAAAAAATAAATACTAAAACTTATAAACATTTAGGCAAATTTCAAAAGTCAAATGAAGACATAGAAAGATCTTTAGAAAACTAAAAATTTGATTATGAATCTAAATTTAAAAATAATAATTAGCTTCTTTAGAAATTAGTAATGCTATAACATTTGACAAAGGAAAAATTGATGGCATTATTAAAATTTTAAATAATAGTTTAAATACTACTAAGTGCTTTTAAATTCACAAAACAAAGAAAAAAATGAATTACAAATGCACAAATTTGTAATTATTAAATATAGACTAGAAACTCAAAATGGAAAAATTTATAGTTCTCAAAAAACAAATAAAACGCTTTGATAATCTGAATATTCACCTTAATAAAAATAGTTTTTTATTTGTTAATCTATTTTTATAGAAAAATAGAAAATTTGCATTTTTAGTCAATTTTGTATAATTAAAGGGCTGACAAAATAAAAAAACGAAAGGAAAAATATGAAAAAAATAAAGAAAATAAGTTTGGCTATTGTTCCTGCAATTGCAACAGTAACAACTTTACCGTTGACTACTATTTCATGTCATAAAGATCCTAAGGAAACTCCAAAATCTCTTACTGAAACAGAACTAAATAGTTTATTAGAAAAAATCAAAGTAACTTTATTAGATAAAAATTTAACTGCTGATGAAGTTATAGCTAAAGGCAAAGACATTTTTAAATTTACAGATGTAGATACTAAAGTTAATTTTAAAATTGTATCAATTAAAAAAGGTGCAAATAAAGGTGAAGTTGAATTAACACTTAAAGTTGTTGATAAAAAAGATTCAAATAAATTCTCTAAAGAAAAAACATTTGAAATAAAAACTTTATTAACACCTGATATGGTATTGAATCAAATTAAAGCTGAATATCCAGATCACTTAAATGTGTTTGTTACAGATGCTAATATAAATAAAATTGTTTACTCTAATTTTGACCAAAACACCTTTGAAATAGTTGAATCTAAACTTTTGAGTAAATTTTATTCAACATTAAAATTTGAATTTAAAATTAAAAACAAAATTAGAAATGAAGTTTCTACAAATAAAAGAACTATACAAATTGATGGTTTTAAATTAACAGACAAATATTTAAATTATCAACTCGGAAATATTGAAATTTATTCAGATGGATCTAAAACTTTAAAAGAAACAAAAACTGAACTTTTAAATCCAAATACAACAACATACAAAACAATATTTATTAATAACTTACCATCAACAGTAAAAATTGTTTTTGAATCTGTTGAAGTATATAAAGAAAATGCTGATCAGTTAGAAATTAAATTTCATCTTACAAATATTCACAATGAAAACAATAAATCTAATACTAGAACTGAATACATATATCACAAAAGTGATCATTATGATGCTTTAGTTCAAGAATTTGAAAAAGCTAAATTATCATATGAAGGCGGTACTAACAAAGTAAATGCTTTTGATATTTTTGAAAGCAAAATTAAAATAGAAGGACTTAAAACACCATCAGATTTTTCAGATTTTGTTTTTAAATTCACAAATTCTAGCCCTAGCTCTACTGAATACAAAAGTGGTAAAAGAAAAATTGATTTTAGTTTTGTTTATAAAGGAAACAAATTCTTCAGAAGTGAACAAGAACTTCAAGTAAGACCAACAACGAATGAAATAATTACATCATTAAAAAAACTTAATAAAATTAAACTTGTTGAAACAACTGAAGCTTTAAATAAATTAGAAAGCTTAGTTGATGGTACAACTTTAGATGCATTTAACAATTCTATTTCAATAGATGGGGTTGAATTATTTAGTTACAATTTTACTGCATATCTATTTTCTGAAGATAATGATACTTCAGCTAAAAAAGTATTACTAAATAAAGATAATGAAAACTATTATTTAAGTTTCTACTTAGGTGGAAAAGTATATGGCAAATATGTAAGACACTATGAAGAAAGTAGCACTACTATAGCTAAAATCCAAACACCTGCTGAATTGACAGCAATGGTAAATTCAAAAATTAATGAATTTGATTATAAAAACAAAACAACAACAAAAGCTAAAAATGCCATTCAAGAAAACATTGTTAGCCCAGATTTTGGCTCAGGTTACAGCGTTAAAATTTCAGACTTCACAACTTTAAATGACAAAATTACATTTAATGTTCAATTAAGTAATTCTCAACATCCAGATGTTTTCTCTAATAAAGTTCCAATTACAATTTCAGGTTTCTACCGTAGCGAATTAGCAGAAGAAATCACTAACATGGAAGTTACAATTGCTGATTCATTTAAAGCTAAAGAAGCTAAAGAATTCACAGATGTAAACAACATTACAATCACAGACAAAAGAACAAGTACTGCTTATGATTTTGCAACTAAAGGTGTTTCTCTTGAAACAACTATTGTTGAAGTTAAAAATTTAGAAGGTATTATTAAATTCAACTTAAAATTCACAAAAGAAACTGAAGTTATTAATAAAGAATACATCATTAATGATTTCAAAAAAGAAACTTTAACAGCTGAAAAAATTGTTAAGCTATTAAATATCCAATTAATTAAAGAAAATGTTCAATTAGACAAAAAATACTTCTTGTCTTCATATATTACAAAAAATGAAGTATCTATTACAAACTTAGATGATCTCAAAAAAATAAATTCAACAGTTAATGTTGTTATTAATAAAACAACACCTAATGTTGCTGAAGGTAAATTAGACATTGAATTAAACATTTCGTTTGATTCAAATTCAAATACTAAAACAATTTCAGTCAGTGGCTTTAACAAAAACGTTAAATCTTCAACTGAAAATATGATATTTAAAGACAAAATGAAGTTAATTGTTTACTTGGCAAAAAATAATATTTTGTTCAAACAAGTAGATCAAAACACAATTAATGAAATGAATGTTGCAGGTCCATTTGGCATGACAACATCTATTAAACAAGGCTTAACAAAATATGGTTTTAACCAAATGACAATGGAAAACAACTTCAATTTTGAATATGTATTTAACTTTGTTTCAGCTGATACTGAAACAGTTCAAGATGAAAAATTTGATACTTTAATTAGACATCATATTGTTGGTAATAAATATAATAAAGATCAACCGATGTCTCGCCACATAAAAGTTTTTAGAATAAATGGTAATACACAATTAGCTGCATCATTTAGAATTGTTGCTAGAGATCATGTTAAATTTGATTTAACTAAATTTGAATATAAAACATCAGTTGACGATGATGAATATAAAGTATTTACATGAATATTAGGTAACATTTAATTAATTTATTATTATATTAAGCGAGATTTTCTCGCTTTTTATTTTTTCTTTAATAACTATAAAACAAACATTTTAGTATTTATTAAATGATTTAATATATATAAAAAAATAAAAGATATTAAATATCTATTATCTAATTATTTTTCAAGTTTTTTAATCATAAAAACTTTATTATTTATCCACACAATGTCACCAATTTTAACTTTTGCACTACGTCCATTTGGTGTTCTATTATTTATTAAAATTTTATTAGAAAGTAAATAACCTTTAGCTGATCCGCCTGTGTCAATAAGTCCTATTTTCTTTAATAATTGACCTAGTGTAATTACATCATCTTTTAAATAAATTTCATTATTCATATTATCTTCTCATAGGAGTTACTAGTTGTTTAGATTCTTTATTTGAATCTGAAACTACTAAAATTTTTTGTACACTTTTATCAAATAGAATTAAAACGTCACCTTCAAACACACTTAAAGCATCTTTTAAGAAATGATAGTTAATATCAAATTCAAAAGCTTTTCCTTCAAATTTAAAATTTTCTGTTTTTGCAATAGATGTTCCGATTTCATTCAATCTATTTAAAACAGTTATTTCATTTTTATTAATTGTAAATTCTAATCTATTTTGTTTATCACTTGTTCCTAATCAAACTTTGTTAATTAAATCATTTATTTCTTCTTTTTTAATAAATATTTTATTTTCAATATCTATTGAAGCAAATAAACTTTCAACATCTGGGTAAGGCAAATCAACTATTCTTGAAGTGATAACTGTATTTTTATATTCAATTCCTATTTTTATTCTATTAAAGAATAATGTTACTTTTTTAGATGCATCTGCAGGTATTAAATCTTTAACATCTTTATTGTTTACTGAAAATTCTTCATTATCTACTTGCGAACTTTTAAGATCCATTGTGTAATATGCAAGTCTTGCTGCATCTGTAGCTGTTAGATTTAATTTATTATTTTTAAATTTAAAATTAATACATTTATATACTAATGAAGCGTCTTGACTAACAGCAAAAGCTGTGCATCTAATAGCTTTTTTAAATTCATTTGTATCGATTTCAATTTTTTTTAAATTAATATTTTCTTCTATTTGTGGAAATGAATTGATTGGGTTTGTAGTTAATGAATAATTTGAATTGCCTTGTGATATTTCTAATGTTGTTGAATATTCTTGACTTAAAGTAATTGTTTCACTTAATTTTTTAATAATAGCTTTAAATATATTTGCTTGAATTAAAAATGTTCCTGTTTCTTCTACTTTAATATCCACATCTTCAACATCTAGTGATTTAACTATACTTATAACTCCATTAGATGCTGAAAATGTTACTTTTTCATTATCTACTTTAATTAATATACATCTGAACCCATACAATGAATTAATAGGATCTGTATACTTTGAAACAATTTCCAATGTTTCATCTAAAATTTGTTTTTTAATGTGTAATTTCATAATAACTCCTTATATAATATTAATAAGCATTGTTATTTTGTGGATAACTTCTAAAAAGTTTATTTTTTATGTTTTTTTTGCTTATTTTTTAAATTTTTGATTAATTTTAATGTTTATATGTAGTTAATAACTATTTTAATTTGTAGATATCATCACTAATCATTGATATGGTTCTTTTAATTGTTTTATCTGGCATATCACTTTCAGTTTCTATTTTTCTAATTGCATTAATAATAGTTGAGTGATCTCTATTACCAAATATATGACCAATTTGTTCTAATGGAACTTCCAGTTGTGTTCTTATAACATAAATAGCTATGTGTCTTGCTAGAACGACATCTTTTTGTCTGCTTTTGCCTAAGATTTCTTTTTTAGGAATTTTGTAATATTTTGCTACATATTCTATTATTGAATCTGGTGTAATATTTTCTTTTGTTTGTTGCATGTCTTTTAATATTGAATTAACAACAGCAACAGTATATTTAGAATTAGTCCGGGCCATTTCCATTTTGTAGAATTTTAATCTTGTTATGGCTCCAATTAATGTTCGAATTGATGTTGAGTAATTTCTTGTAACGAAGTTTTTAGCTTCTTTTTCTCAAGCCTCTGGATTCATATCATTAATATCTAGCATGTAATCTAAAACTCTTAATAAATCACTTTGTTGTGGAATTTTAATTTCAAGTTGTAAACCCATATAAAGACGAGAAATTAATCTTTCATCAAACATCGCATTCAAGTTATTTATAGGCTTATCCGAACAAAATATGGTTGTTTTATTTGTGTTCAAACGATAGTCTAAAATATTATAAATTGTTTGAATTGTTGCTTTTTTATTTCCGACTCCATAACTTTGAAAATCATCAAATATTAAGTAATCAGCATTATCAAATTCATCTTTTATTTTCTTTAATTTTGCTTGATCATTTTCTTGCAAATAGAATGATATATCTCTAGCATAAATGCTTGGATTAATGTATTTAACAACTTTGCCTTGCTCTTGTAATTTGTTACCAATTGCATGTAATAAGTGTGTTTTTCCTAGTCCACTTTTAGCATAAATAAAAATAGGATTATATTCTTTTCCACCTTTAACTAAATAGTTAGCTAATTTGATTGCATCTTTATTAAAATCACTTTCAACATAGTTGTCAAATGTTAATTCTTTATTTAAATCAACGCCTAATTTCACTTCTTCTTCAATTGTTTTAGAAGAAATTGTTTTGTTGCTTGAGGCTTTTGTTAATTTAACAATAGGTTTAGGAATATGAACAAATTCATATGTGCATTCTCTTTCAAAAGTTTCTTCTAAAGCTTTTAAAATATTACCATCTAGCATTTTTATTAATATTTCTAAGCTTGGTTTTTGTAGTCATGTTTGAATTTTGACATGATTGTTCTGATCTATGCCTACAATTGTGAAGTTTGAAATGAAATTTTTGAACAACATTTGGTCATTAACATCTGAACTTAAAGATTTTAAAAATGTTAATGTATTTGCTTTCATTGTGATACTATCTTTTATATTTTTATTCTCTGCCATAATATAAAAATAGTATTATTTATATTTTAATGTAAGAAATAATTAGTAAGTTTTTTAGTTATTTAAACAAGTTATCCACAAACTTATCAACAATTAAAAGTCTTATTTATAGTATAAAAAGTAGAAAAGTTAACTTATTAACATATGTTTATAACTATGTGGAAAACTGTGTTTAAAAATAAGAATACTCTTTATATATACTTAAAAATTTGATAATATCCTTATTTAAAGACAAAAAAATATTTAATGAAAATGAATATTAAACTTATTTAAATGAGCTCAATAATGTTGTGTTTCAAATTTTATCAACTTTTATAGCTACAAATATGTTACACCACTATAATTCAGCAATTAGATTTAAATTTAGTTTCAAAAGAAAAGAAGATTCAATTTCTAAAAACTATATAAAAATTTAGTAGTTAATGATGGTAATTTGTGATCAAATAAGAATAAAACTTATAATTTAGATCTAAAGAATTATCTTTATAGTGAATTCATCGTTTAAAATAATAAGTACAAACTACCAAGCATATGAAACTAGAAAGTGGATTTTATATTTTGTTTTTATTATTTTATTTTTTAAAATTAAGCATATTTATTTTTATATTATTCAATATAATTTAATAATATGCAAGCAAAAACAACAATAAAAGTAAAAGGAACAATAAAAAAAATAAGAAAAGGATTGGCTCAAAAGTGAATTCTTTTTGAATTTAAATCTCAAGAAAACCAATTATTTGTTGTCTATGCTCGAATTAGTTCCGATGACTTTTTACAAGTTGGTTATTTATATGAAATGGAATTAAGTGGGCCTAATAAATATAATAATTACATACTTGAAGGCTACAATATGATTGAAGCAACAAAAGAAGATTTAAAAGTTATGTTGAAGAATCATATTAGAGGTTTTAAAGAAGAAGATCATAATATTTTAAAAGAAAATTTAGGTGAAAATTATTTAGATGTTATTAAAGATACACCTAAAGAAGAAAGAAAAATATTTTACGAATTTATTAAACCCCCTTTGCTTAAAAAAATAGAAGCTTTTATTGAAGAAGTTCTAAACATGGGTAAAGATGAAAGATTTTTTATAACCAATAATATTTACAATTTTTATATTAAATTGAAGCAAAAATTACCTCTTGCAGATGGCGAATCATATTTAGATTTTTTTGATAAAAACAAAGAAAATCATGAAGATCCTTATCTTATTTTGTATATTGAATTAGGATTAAGCTTTGACTCAGTAGACAATTTTGCAAGCTTAATAGGATATTCAAAAGATTGCGAAACTAGATATTATGCAATAGTTTATAAATCTATTTATGATTTTGGAATTCAAGTTAATGATACATATTACAATTTGTCTGATTTACATAAAAATTGACTTGAATTTACAAATGAGTTAATACTAAATAATAAGTTATCAATTTCAAAGCTTGCTATTGATAATATATTTATTAATTCTATAAACAAACTAATTGAAATGCAAAAATTATATTATGCAGAAAAAGAAGTTCCAACTATAGCACTTAGTTTTATTAAAGAACAAGAAGACTTTATTTTTGAAAAATTAATAACTATAAAAAATAAGATAAATAAAACTATTCCGCAAATAGAAGATGATACATTAACTGCTAAACAACTTGATGCTTTAAACATTGCACTAAATGAACCGATAAGCATAATTTCAGGATTCCCTGGCACTGGCAAAACATATTTAATTAGTAATATTTATGAGTCATTAATTGCCCAAAAAGTCTATAAAAAAGGTGAAATAGAAATTTTGACCCCCACTGGTAGAGCTGCAATTAATATTAAAAATAAGAGTAATAATTTGCCTGCAAAAACGATTCACAACTATTTAAAAATTGATAAAGAAGATGAAGACGTTGTTTATAACAATGATTGTGACAAAACGAAAGTTGTAATTATTGACGAGTTTTCAATGGTTAATTTAAAGATTTTTTATCAACTTTTAAGTCATTTATCCAAGCTTGAAAAGCTCATTATTGTTGGAGATAAAGATCAGCTTCCATGTATTGGACCTGGCAATTTAATGCACGACTTATGTAATTGAGATTTTATTCCTAAAACTCTTTTAATTGAAAATCAAAGAGCTGAAAGTGATGAAATTTGCGAACACTTTTTAAGTATTATTGATTCAAAAGTTAAAGAAGTTGAAATAGGAAGTACAAACACAATTCAAATGAAAGAAATAAACTCAGTTGCATTATTAAACGAATTATCATACTTATATCAAACTTATAATCCAGAATACAATTCTGAAAAAATGGTAACCTTGATTCCACTTCATGATGGTCAAGTTGGAGCCAAAACAGTTAATAAATGATTGCAAAAAGAAAAACTAAATAATTACACATATAAGTTGCCTAAATTTAAACAAAGTAAAGACAACTATATCCAATCTTGAGAAGGTACAAAATTCTATTTATTTGACAATGTTATCCAATTAATAAATGATTATGAAAAAAATGTTTTTAATGGTGAGATCGGAATAATAAAAGAAATAATAGATGACAAAATTGTTAGTGTTGAATATCCACAAGGTGGAAATAAATTTAAAACAGTTAATTATGTAAAATCAGAAATAGCAATTAATTTATCTTTAGCATATGCTTTAACTGTTCATAAATTTCAAGGTTCAGAAGCGCCACATGTTGTAATTCCGGTTTTTAAAGATTATTCAAGAATGCTTAATAAAAAACTTTTATATACAGCAGTTTCAAGAGCTAAAGAAAAATTATTTATTTTAGGCGACTTAGCTTTATACGCTAAAAAAACAAAAACTGAAGATTATAGTAAAATAAAAACATATTTATTTGATTTAATTAAGGAACAAAATTAATAAATAATTTATTATAATTTTCATAATGTTTATAACTAATACAAATGAACGAATGGTTTTAAACAAATTAAAAAATCACAACGTGAATGGTGTTTTAGCTGTTTTTGAATCTTTTGATATCAACAAAATGGTTTATAACAAAATAAAAGGTTATATTTCACATCAAATTTGAATTAGAAGATCTTAAAATTGGCACATTGTGTTCAATTAATTACAATTTTGATGATAAAAAAAAGATCATTAATTATTATTCTGAAGATTCACAAGTATTAAAACTTAAAGGCTCATTCCGTGCGAACAAAAATATTGAAGAATTCTATGAAAGTATTTTAGAAAACAAACCATTTCACTTTGATGGTGAAAATGTAATTGCATTTAATCAAAATGATATGGCTAGAAAATACGAAGCTCTCAAATATTATTATAATTATATTGACTCGAAGTTTATCGATTCATCAAAAAATATTAAAGAATGTTTCCCAAATTATAAAGATGAACTTTTTGTTATTTATGCACTTCTAAAAGAATTTTATGGGTGCAATTATCCAGAAGGAAAACCAACTGAGTTGAAAAAGTAGTTGCTTTGTTTCCTTTAAATTCACAAGTTAGAGATGTTATTCACAAATTAAATAATATTGATTTTTATTTCCAAGGTGTAATGTAAAATTTATTTAGTTCCTTTGTTTATGCTTTTATTCAAAATGTAAATAAGTATCATTTAAACATTTATAATAGTGAATTAGGTCAAGCTTTAGAAAATTATAAAAGAGATCTAATTGAATTAAGATTCTACAAAGATGACTTAGAAAAAAAGATCATTTACAATAAAAAAAGTGAAGATTTTTTATCAGCTTATTGTTTGAATTATTATAAAGTTCAAGGTTCTGAATCTGATGTAGTTATCATTTACTTTGACAAAAACATAGCCAAATATATTACTAAAAATCACTTATATACTGCTCTTTCAAGAGCTAAAAAGAAAATCATTATTATGAATGATGTAGATGAATTTAAAGAACAGTACCTAAACTAAAAGAAATTAATGTTTTAACAAATATGAATTTTGAATTTAAAAATAAAAATAATTTATAATTAACATATGAAATTAATCGTAGGTTTAGGTAATCCAGGAAACCAATATCGTTTTACAAAACACAATATTGGTTTTTTAACTGTCGACAAAATTTGTGCGAACTTTAATTTAACTCTTACAAAAGAAAAATTTAATGGCTTTTATGCAAAAACTGAAGATTTTATTTTAGCTAAGCCAACTACTTATATGAATAATTCTGGTGAATTTGTTCAAGCGATAGCTTCATTTTATAAAATAGATCCTTCAGATATTTTGATTATTCATGATGAAAAGGATTATGAAATTGGTCAAGCAGCAATTAAAGTAGGCGGTTCAAGTGCTGGACATAATGGTGTTCAAAGCATAATTAATGCTCTTAGAAGCAATGAATTTAAGAGGCTTAGAATAGGAATCGGAAGAGATCCTAAAATGGAATTAAAAGATTATGTTTTAAGTAATTTTACAAGTGATCAAATGTTAGTAGTCGAACAAGTTCTAAATGTTGCTGCACAAGCTGCAATATCTTTTGTTTACAATGATATTAAGATAGTAATGAATAACTTTAATGTTTTTCGTAAAGGTAAATAATATGTATATTTTAGGAATTAGTGGCGGCCCAGATTCAATGCTACTTTTACATGAATATGCTTATAAAAAAGAGCCCATTTTTGTAGTGTGCATTAATTATAATCAAAGGATGGATTCACATAAAGATGTGGAAATTGTAGAAAATTATTGCAAACAAAATTTTATAGATTATCAAATATACAATTTTGACCAAAAAGACTATAAAACAGGCAATTTTGAAGAATGGGCCAGAGACAAAAGAATGGAAATCTTTATTCAAAATTATCATGAAAAGAATGCTTCTAAAATTTTGTTAGCACACCACAAAGATGATTTTTTAGAGTCATTTTTTATGCAAAAAGTAGCGAAAAGAAAACCTTCATTTTGAGGCATTAAAAAAACAAATATTTATAAAGGCTGTTTAATTGAAAGACCTTTTATTGATAAGTATTTTAAAGATGAAATTTTAGAAAAATGTAAAAAACTAAAATTAGATTATGCAATTGATTACACCAATGAATTGCCTATTTATACACGCAATAAATTTAGATTAAAACTAAAGAAATTAACTAACGAAGAAAAACAAAAAATTATTGATGAAGTTAATGAAAAAAACAAAGAATTAGAATGATTAGAAAAAGAATTTAATAAAGCGTTTGCAAGCTGAAAAAAAATAGAATTCAAGGTTAATTATTATAAAGATCTTGATTATGAAATTCAAGAGCAATTAATTAGAAAATTTATTTTTGCTCATTGCGAAGATAAAATCAATTTAACTTCAGGCAAGATTAAAAATCTTGTTAATTATGTAATTGCAGATAATACAAATAATCCTATTTTTATTTTAAACAGTCAATATTATTTGCAAAAGTCGCAAGGAAAATTATTACTAAAAAGTAAAATTACTATTTAATATAAATATTAATGTATAATTTGTTTTATTTATTAACCAAATTAAGAAAGGAGCATTTATGCAAAAAAAACCAAGCATACTGTCAATTATTTTAATTACAGTATTGTCATTCTTAGCTTTATCAATCGTTTCATATTATTTGTTTAAAAGATTTGGACCTAAGCCTCAAATTGTTGACTTAAATCAATTTATGAATGATTTAAAAGCTGCTTCAGCTAGTCAATCAGATGATGTTTATTTTGCAAGTATAGAGTTTGACTCGTATTATGGAACTGCAAAATATGTATATGCTGGTTTAGACTTAAAAGGTAACCGCATATTAGTTGAAAGAATTGCTCAAATTGGAAGATCTAATGCAGCATCATGATCTTCAACTAATGAAATTATTGGAGAAGGATTAGCCAATGTCTTAGGTAATAAAGCTGGTTTTAAAGGATTAACTTTTTTTGAATTCTTAAAAACTTTTGTCCTAACAAAAGGTGGATCTTACTTAGCTAAACCTATTCCCGATGGACAACCTTCATTATTAAGAAGTTTACTTACTTCATTAATTCCAACATTTATTACTATTCTTTTATTATTTTTCCTTTACAGATACTTGATGAAAAAAAATATGAGTATGATGGGTGCAATTGGAGATGTAAAGAATCCTGCAAGAAAAGTCAAATCAGATAAAACATTTAAAGATGTTGCAGGTAACAAAGAAGCAATTGAAGAAATTGAAGAAATTGTTGATTATCTTAAAAATCCTAAAAAATATGCACTTGCTGGTGCTAGAATGCCACATGGTATTTTACTTGGAGGCCCTACAGGGACAGGTAAAACATTGTTAGCTAAAGCTACAGCAGGTGAAGCAAATGTACCTTTCTACTTTATTTCAGCTTCAAACTTTGTTGAAATGTATGTTGGTTTAGGTGCTAAAAGAGTTAGAACTGTTTTTGCAGAAGCTCGTAAAAATGCTCCAGCTATTATCTTTATTGATGAATTAGATGCTATTGGTCGTACCCGTGGAGCTGGTTTAGGCGGAGGTCATGATGAACGTGAACAAACACTTAACGAACTTCTTGTTGAAATGGATGGTATGACTCAAAATAATGGAATATTAATTTTTGCAGCAACTAACCGTACAGACGTTTTAGACCCTGCTTTAACTCGTCCAGGTCGTTTTGACAGAACAATTACAGTTGGATTACCTGATGTTAAAGAACGTGAAGAAATTTTAGAATTGCATGCTAAAGGTAAAAGATTATCACCTGAAATTAAACTTGATCAAGTTGCAAAAAGAACTCCAGGATTTTCTGGCGCTCAACTTGAAAATGTTATTAATGAAGCCTCATTATTAGCAGTTAGAGCTAACTCAGACGTTATTAGACGTGAAGATATTGATGAAGGTATCGATAGAGTTATGGCTGGACCTGCTAAGAAAAATAGAGTTATTACTAAAGAAGAACTTACAATGGTTGCTTACCACGAAGCCGGTCATGCTGTTGTTGGATTAAAAGTTAAAGGTGGAAACAAAGTTCAAAAAATTACCATTATTCCTCGTGGAAATGCCGGTGGTTACAACTTAATGACACCCGAAGAAGAAAAATATAATGCAACAAAACAAGAATTGATTGCAATGATTACTGGATTTATGGGTGGTCGTGCATCTGAAGAATTAATTTATGGAAAAGACAATGTTTCTACAGGCGCTAGTGATGATATTTCTAAAGCAACCAAAATTGCTCGTAGAATGGTAACTGAATTTGGTATGTCACAACTTGGCCCTATTCAATATGAAAAAGATGAAAGCTCTCCATTCTTAGGCCGTGATTATCTAAAAAGTGCTTCATTTAGTTCTCAAGTAGCCCACGAAATTGATCTTGAAGTTAGAAGAATAATGACAGAAGCAGAAAAGAATGCCGCTAAAATCATTAAAGAAAACAGACAACTTCTTGAATTAATCAAGACAGCTTTACTTGAAAATGAAACAATTGTTGCTGAAGAAATTGAATACATTGAAAAGAATATGAAACTTCCTCCAAAAAGAGAAGCTGACAAAATCAAAACCAAAGTTTATACTTTAGATGAGCTTATCAATGAACACAAAGATGACAAAAAATTAGAAGATTCAAAAGCTGAAACAAAAAAAGATAATTCTAAAAAATAATCATAATTACTTGCTTTAAAAGAGCAAGTTTTTATTTGAAATTTTGACTAAAAATCCTATAAAAAAGATAATTCTAAAAATAGAAACATAATCTATTGATTAATAATTTTTACAAAAATTAATTTTTGTAATTTTCTATAAAAAATTAAATCTTTAATATTAAATATTTTTTATTATAATAATACATATTAATTATTAGGAGAAAGTATGTGAAGATTATTCAAAGAAGTTTTTAAATCTCTCTCTAAAAATAAAGTTATAGTTGCTGGACTAAGCATTTTAGTTTTTTTAACTTCAGCAATTTTTACCTTGCTATCAAACGTTAGCTCTTCAATGGTTAATGGTTTTAATGATTATAAACATGTATCAAAATTGCAAGATATCAATGTTGACTTAAATTTACCAACACAAGGTAGTGCATATAATCAAGGTTACTACATTAATGGTGAAAAATCAGATGATTTCAAAGGGACAAATAAAGACTATACACCAATTGAATATCAAATTGATTATCAAAGTGTAAAAGCTTCTTCATTGCCCGATTCAACTAAAAAAGAATATGAAGACTTTACAAGAAATGTTTTATTTTTTGGCAATGATAAAAGAGAATATTTGCCATTTAATAAAATAGGTAATATTGACCCTTCGGTAAATAGTTATTACTTTAAAAAAGATGACTTATTAAATCTTTACTCAATTTATAAAGCTGAACAAAACATTGAAAATAATAAAAACCCAGACATTGTTTTTGATTTAAAAGACATAAATAATTTAACTTTTGAACTAAAACAAAAAAACCGTTTCTTGAATATTTATGAAAAGAAAGGCGATACTTATGAACAAGTTTTTCTTGAAAAGAAACTTGAAAGAACTAACATTGTTCATTTCGATAAAACCTATAAAATAGGTAATTTAATGAATTTAACTATCAACAATAATGAAATTTATGCAACTCAATTAAGTTCATTATTTATTAATACTGACACAAAGGAAATAACAGCAGACTATTCAAAAGGTAAAAGTTGAATTAACCAAGAAAAAGGTATTGAAATAGAACCTAAACAACTTGCTGCATTATTTAATTTTGAACCTACTTCATTTGATGGCAGTACTCCAAATGGCTTACTTTTTAAACAAAAAGATAAAACAACCAATTTAGATAAATTTATTAAATATAATCCAAGTTGAGCTGAAATAAATGAAAACTTAAATTTAAAACCTGACTGAAATCTTGAAGATGTTTTTGCAGCCAACCCTAAAGCAATAAAAGCTCAAAAGAAATTATCTTTAAACAAAAATAATAAATTTACTTTACCAATTGAATGAATTGCTAAAGAAGAAAACATAGTTCGTTTTTTAAGATGAAACTACTACACAAGTTTTGTTGATAAAGATAAATCAAAAGATAAATGATTAGGTGCTTTCAAAACATTTATGGAAGACTTAATTGAGTCTTCAAAAACAAATCCAGAAAACAAAAAAATGTTAGATAAATTAACAACTTTTTCATATTGAAGAAAAAGTAAAAAGAATATAGTTACCCCATACAAAAGTGACAAAACATTAGATTTAAGTAACAAAATTACAAAAGAATCGGTTATTCCATTAGGCTCAAGTTTTAATTATTTTGAAACAACTCAATTAGAGTTATATACAAAAGATTCAAGAATTAGACCAGATGGTCAAAAATATTTAATTGGAAAAGACACTCCTAGAACAATTAATAAAATTGAAAAAGAATCACAATTCTTAAAAGATAAAGTTAGATTTTTAGATGAAATTAACAATGAAAACATTAAATCTGAAAGATTTGCAATTATTAAAAATGAAGCTTATGAATCAACAAAAAGAAAAATCATTGATTTAATTGTAGCAAAAGTTAAACAAGATAATATCGGACTTCGTAAAACTTTAACTGTTGATGGAATTAATGAGGAAACAGGAAAACAAAATGTTTTCCACTTTATTAATACAGGTGATGAGAATTATGTAGTTGATGGTGTTAAATTAAATGTTGGTAAATTATATGATGAATATTATGATCCTTCAATTTTAAATAAAGCAACCGAAAACCAGAACAACATTTATAAAACTCATCAACTTACACCTTATGTTTCTTCTTTAGTTTTACAATCAATTGGACGTAACTTATATCCTAATCCTGAGTATATTAGACCTGCATATCATTTTGGAAAATTAATTGACTACAACCAAAAAACAGGTGAATTAAAAGAAACAAAAAATGCAAAAATTGTTCCTTTAACTCAATTTATTCCACACGATCAAAATAAAGATGGTTCGAAAACTCCAGATTATAATTTAACTAATCTTGGTGTTGCATTTTTTGGAAGCAAATACAAAATCGTTAGAAGACAAAAAGATGTAAACAATAAATATGTAAGCCCTGAGACATGAGAAGTTGTTTATACAGAAAAAATGCCACAAAATGGTATGGATAAGGGTCTTTTGTCAAAATGAATGGAAAAATATCAATTAACTATTGCAACCAAATTTATTAAAACAAGTGGTAATGGTTGAGTAAAACAAGACTCTACTTTCCAAAATGTTTCATATATCCCAATGCTTTTTTTAAGTCCAAAATCTCAATTAATTTATGATGTTTTAAATCATGGAAAAATTGATTATTTAGCTAATGCAATTGAAAAATATTTATTAAATTTTGATTTAGTTAAACAACAATTTATTACACCTGAGCAAGTTTATCAATTAACAAAAGTTATGAAAAAAGTGTTAAATGATAACAACTTTGCTTCAGTGTTTGCTACTGGCAAAATGAACAAGAAAATATTACCTAAATTAATGTTTGACTTTGTTTATGAGTTAAGTCATTATGAAGGTGGTGATGTTCTCAAATCACTATTCTTTAGCATTCTAGAACAAGCAAAAGCTAAAATAGCTTCTAAATCAACTTTAGTTGAACAAAAAGCTTATTTAGCAGCTGAAGTAAATAACTTATACAAATTACTACAAGATTTAGCTTCTATTGATTTAAACCAATACTTAAGTGCAGAATCATTAGTTAATGTTTCAAATGATCCAAAAATATTTATTAATGCAATTTGAAAAATAATTGATTCAATAGATTTTAAAAAATTTAGTGAATACTCGCATGAATGATATAAAAATGAAAGTGATAAAAAAGTAGTTCATAATGGTAAAACATACACTCAAAAACTTTCATCAGGTTTATTAATCAAATGATTATTCAATTCATTAGACCAAAAAACATTAAAAGAAGGTCTTAAATTATTAGTTGATAATTTAGATATAGCTGCTTCAATTAATTTAGATAATAGTGATAGTTTACTTTACAATTTATTAATTAAAATGAATCCTTCATTAATTAGTGGTTTAAAACCTATTATTAAAAAAATGAATAGCGATCCAACAAATCCTTATTCTAATGTAAAACAAGGCTTAATTAATATTCTATCAAATATTGATTTTAATATTCTGGCTCGTGAATTAAATCAAACAATAACAACACATTATATTGATTACAAAGTAGTTAAATTTAATCCAGACTTAAACAAAGATGAAGAAAAAGTCTTTTCAGTTGTTTTAAATGCAATAAGTCCAAAAGATGGAATTATATCATTCTTAAGATCTATGTTTGGTATGCCTGGATCAAATAGAGCATTTAAAGAAAACTTAATCAAAATGTTCAATTTGTCTGACAAATATAAAGAAATAGAACTACCTAATAGTAGTGTAAAAATTTATGTTCCAGATAATGACGACACAAAAGTAGGTTTCTTTGATTTCTTAAAAATTTTTGCTTCAGGTTTATCAAATACAGAAGCTAATGAATTCAATAACTACAAAATTGAACATGACTTCTTAGCACTTAAATCATCAATTGAAAGTCTTAAAGAGGAAGGCACTCAAATTAATTTAGATTACTTAAAAGAACCTCAAAAAAGATTACTTAAACAATATGGTTTATTTGTCACAAAAACCTATAAAACAGTCATATTAGAAAAAATTAATAAAATATTAAGGTTCATAAAACAAACAAAAGGTGGAACTTCATATATAAGTAATGATGCTAATAAAACAGGTAGTGATTTATTAAATGATTTAAAAAATTTTGAAAACAACGGGACTTGATCATTAATTAAAAAATTATTATCTTCATTAACAACTCCAGTTGTTCAAAATGAATATGCATTAGGAGCTCAAACATTTTCAATTTATAATCCATGAATTCAAATGTTTATGAATAAATATGCAAATCATGAAGAAGCAAAAAGATTTGTTAAAGATTTAATTGCATTAGCATTGGAACCTGAAATATTTGACATTAACAAAAAAATGTCAACAAATGACAATATACCTTTTGCTGGTGTAACTAACTATGGATTATTAGAATTACTTGAAAATCCCGAAAAATCAAACTTATTTGCTTATGATGTTAATGGCAAATTCTTAAATGACAAAGTTGAACAATTTGCTGCTAAAAATGAAATATATAGAAAATGAATTCAAAATAATAAATTATTAATATTACAACAATTAGGTTATATTACAGCAAGCAAACTTTACAGTAATTCTTCTGAATATCCTGATGGTATTTATTTAGGAATAATTCAAAAATTCTTGAACAATTATTTATTAAGCAAAGATTTTTATGACATTAAAGACCAAGCTCAAATCATTATGTCTTCATTAAATCTTAATGTACCATTACAAATTTTTGGTTTAAGTGAAGCCTTAGTCAATCCTATTTTAAGATTTACTTTCCCTGAAATTACATTATCTTATTTAGCAAGTCAAAAAACAAATGCTAGTTTAGTTAAAGCTAACTTACAATACTTATTGCTCAATAAAATAGATGATTTCGAAAAAATTGTTAGAAAAGGCGAAGACAAATTTTATGCCTTAACTAACATGTTAGGAACTGCATTTAGCAAAAAAGATACCTCACTAATTCCTCTTGACTTAGATGACGAACAAACATTAGTAATGGATGGAGCTACATTTGATAAATTAAAAGATACAAGCTCTTCATTATTTGGAATTGATTTTATTAAATTTGTTAATGAAGCAATGAACACGATAGTTGAACCTAAAGAAATGAAGGACATTGTGTTCTCAAATTCAAATAGTTACTTAGCTAAAGTTAACTATGCATACTTAGCAAAAAATAATAAAGCAATTTATACAGGTAAAATTCCAAGTGATCCTATTGAACTATTATCATTATTAGATAAATTAGATGAAAAATTCATTTTAAATGTTAATGGAACCAAATTTATTATTATTGGTCAAGAAACTACAGTCGACTATATGTACCCAGTTATTGATGAAAATAACTTACAAGTTAATACACAAAACCAATCCTTAGTTTATGTTAACAAATATGGTTTTGACAGAATTCACTCAGGTTACATTGGAAATGTTATTAAGAAAACTCTTTTAGTTAAAAACTCAGCAGCAACTGGTATGAATAATAATGAACTTAAAAAATTCATTACTAAAATAATAGATAACTCAATTGCAGACAGTAACAAACTTCAAAGAGTTTTCTTAGCAAATGAAATTGATCCAATTAACCCTGAAAGGTCTCTTAGAATAACAACTGTTCAAGGAATTATTAAAGCTGTTTCATCAGCTACTATTGCCTTAGTTTCAGTATTCGTTACACTTGTGGCTATTTCAACTATCTTTATTATTAAGAGATATATTTCAAATAAAAACAAAGTTATTGGTATTTTAGTGGCCCAAGGTTATCGTCCATGACAAATATCACTAAGCTTTACAGTGTTTGCTTTAGTTACATCAGTTATTGGTGGAATCTTAGGTTATGTAATTGGTTATAAACTACAACTGATGGTTATGAACATCTTTAGTAGTTATTGGACACTGCCAAAACAAACGATTTCATTTAACTTCTTTACATTATTCTTCACTGTCTTCTTACCATTTATTGGAATGAGCTTATTGATCTTTATAGTTTCATTATTTGCTTTAAGACATAAAGCTATAGACTTAATTAGTGGAGTTAATGAAATACCTCAATCAAAATTATTCAAAACTTATCAAAAAGCAACTAAGAAATTTAATGTTAAAAAACGTTTCTCATTTACATTGGCATTTACAAGTTTTTGAAAACTAATTTCATTTACAGTCAGTGTTATGTTAACAGGTATCGCAACCTTATTTGGTGTAGCCAATACAAACGTCTTTAATAGAACTATTAATGATACTTATAAAAATAGAAGTTATACATTTAAAGTTGATTTAGAAAGTCCAACAGTTGAAGGTGGAGCATTCAAACCATTTAATCCTGATAATTTATATAACAACATTTATACTCCTCTTGGTGATATAAATGAAGGTAACAGAGAAATAGGAGATTACTTTAAACCTGGTGCTTCTAGTGTGCTTAATAAAGGAACTAAAAATGGTAGTCCTCAAGATTTAGACTCACATATCTTAAGCCAATTCTCAGTTAATGTTACTGTTGCAGCTGGTGTAGCCGCTGACCCTTGACAAGTAGCTTACAATGGTATGCCAGATACTCAAAAAGCTAAAATTGATAAGATAAGAGACCGTGTTGGTTATGAGTTAGAAAGAACTCAAGAAGACAAAGATAAAGAATTCTTTGTAGATCCTAATACTTATGCTTTAAGCTTAGTAAATAAGGCTAATAAGGAAAAATTAAGCTTCTTTAAATACTATCATTCACCATATGAAAAACAAGGTAGTTTCAAATATGCTTACTGAGATAAAGATAATGCAAATTATCAAATGGTAAATATAACAACCGATAAATATCGTGATGAATTTAGAACACTCTTAATCAATGGTTATACTAAAATTGCTCAAAAAATTAAAGCCGAAAAAGAAAATCCAAGTTTAATTAAACCTAAAGAAAAGAAACCAACCAATTTATCTGATTACTGATTAACTGATAAAAGTGATTTAAGTGGTCCAACTATTCAAGATTACTTTATTAGTTTTGGTGGTGTTTATATTGACCCAAATCATGATGAAACTTATACTTATGTAAAAGTTGCAAACAAAGATAATAACTTCAAAATTTATGGTTATCAAAAAGATAGTAAATACATAAAACTTGTAGATAAAAATGGTAATAATTTATACAATGATTTATATAACTATAAAGTCAAAAACAACATTTATCCTTTGGTTGTAAATGAAGTTACTTCGAAGAAATATAACATTAATATAGGCGATTTAGTCCAATTTGAAGTTCTTAACCATACAAAACGTTATGCAAACAAAGTGTTGTCAAAAATAGATAAATCATTAAGAAGTAATGTTGACTTAAAAAATCCAAAAGTAACATTTAAAGTTGTTGGTATTAACCCTACATACATTAATAATGAATTAATAACAACAGTTGATGCAGCTAATAAATTAGTTGGCTTAGACAAACTTCCAAATGTTGTTGGATTTAAAGCCTTCAATGGTGTTATGACAAATAGTCCAGCGCCTAGCCAAGTTCTTGATTCAACTTCGCTTTATTCATTAAGTGGTTATTGATCGGGATATGATGGTTTTGATTTAAGCGGTATCGATGACAACACACTTAAATCAATGTTTGAACAAATTTATAATCCAGATACTGGAATATTAAAAACCAGATTGAATCTAACAAAAGATCAAATTATGAATTTATTAGATTCAACTGAATTAACATTTAATAAAGCTAAATATGATCAAATTAAAAATGCGCCTAAAAACGCAATAGAAAATTATGCTAAATTGTATGATTCTAAACATTATATTGCTTTAGGTACAAGTATTGATTCAAAAGATATAGAAGCTGGATTTACAACCCAAATTGGTTCTACTATAGGAACAATTAGTATTGCAATTATTGCAATTAGTTTCATAATTTCACTTGTTATTCTTATTATTATGTCAACAATTATGATTAGTGAAAATGAGAAAAACATTGCTATTTGATCTATTCTTGGATATACACAAAAAGAAAAAATTAGTATGTTCTTCAGTGTCTTTATTCCATTTATAGTTCTGGCCATCGCTCTGGCGATACCAATAGTAATACTGATGATATTTGCATTTAACAAGTTCTTGCTGGCCTCCTCGTCAATTGCCTTGCCATTAGTGCTTACGCCACTACATATATTCTTAACAGCTTTAGTAATTTTTGGAATATTTATTATTACATCATTTGCTACCTGAATTAGTATTAGTAAAATGAAACCAGTAGATTTATTGAAAGGAAAATAGTGTGTCTGAAGAATTAGAAAACAAAGAAAAAAAAGTTAAAAAAGATCAAACTTCTACTAAAAAATCAACAGCCAAAAAAATGACCAAAAAGTCTGCAAAAAAGGCTGAAAGAGAAAAAAATAAAAAAGAAGAAACTGGTCTTTTACAAGCTATTATTGAAGAAGAAAAACTTCTTGAAGAACAAGTCGCTGAATTAGAAATTGACAAAAATATCAAAATTACTAAAGGCAATGTTTTTGAGCTTTTAGACAGAGATAGTTCTAAAAAGAATATTATTGTTCCTAAAAAAGTTCGAAAATTATTGACTAAGTTAGCTAAGAAAAAAATAAAAAAAGATGGCAATGAAAACCTAAAAAATGAAGAAGGTAATGTTATTGAAGTTAAGAACGTTAGCAAATATTATGTTAATGGTAATATTGTTACAAGAGTTTTAAAAGATGTTTCAATTGAAATTAAAAAAGGTGAGCTAATGCTTATTTATGGTGTTAGTGGTGGTGGTAAAAGTACACTTCTAAACCTTATTTCAGGACTTGATAGACCTTCAAAAGGACAAGTTATTGCTTGCGATAATAATCTTCCTTATATGTCTGATAGTCAATTAACTCTATTTAGAAGAAAACATGTTAGTTTCATTTTCCAAAACTATAACTTGTTAGCAAACTTAAATGCTTTTGACAATGTTCAAACGGGAGCTTACTTACAAAAAAATCCTGCTAAAAAAGTTGATGTTGTTGAGTTATTTAAGAAATTTGAAATGGATGAAGAAATATCAAAATTCCCTGCTCAAATGTCTGGTGGTCAACAACAACGTGTTTCAATTATGAGAGCCTTAGCTAAAAATGCAGATATCATTTTTGCTGATGAACCTACTGGTGCTCTTGATGAAGCAACAACAAAAATAGTTTTAAGATTTCTTTACAATATTAATAAAGAAACAGGCGCTACAGTTGTTATGGTTAGTCATAACCCAGTTATGGCGCAAATGGCTGATAGAATTATTCATGTTGTTAAAGGAAGAATTGCAAAAGTTGAAGTTAATCAAAAACCAATTCACCCTGATGATATTGAACTTTTTAAAGAAAAATAATAATTTAGATATTCTTTGCTTAATGCAAAGGATATTTTTTGACCAAAATGACTATAAAAAAGGAATATTTCATACAAACTTTTAATATTTTTTTATTTTAAAAAAATGTATAATTATTAACATGAAATTTTTTAAATCAAGAATACTACCTGCTTTTATTTTCGTAGTAGTATTGCTAAGTTGAATGGTACCTTTAGCAATTTTTGGAAAAAATCATTATGAAGCTAGAATAGTTTCATATATTTTTACTGCTATTGGGATAGGAATACTATGCTATGAATATTTCAAAGCTAATCATTTAAAATGATATGTTATTGCTATTTTTATTATGCTTATACTACCTACTGTAGTTTTACCGGTTAATAACTTTACTGAGTTTATTTTTGTTTCTCATCCTTTAGAATTTCATCCTAGTTTTATTCAAGAAATAGCAAAAGAATGATTTACACCAGTTGTTTTATTAATGATTTCTGTTGCTTTTATTATTATTGAATATATAACAAGAAATAATATGAGTTTTGAAGATCGTTTGGTTAGAGGAATACTAATGTTTATTTCTCTATACGTAATAGTCAACACTTCAAAATTGATTCAAATAACCATTATACTTGATTGAAGATATACCATCCTATTATTTGTTATTCCTTCTACTTGTGATATCTTTGGTTTTGTTGGAGGAACATTAGCAGGCAAAAAATGAATCAAACAATCATTTGCTCCAAATGTTTCACCTAAAAAAACTTGAGAAGGTTTTATTTTTGCTTTAATTGGTGGAATTGGAATTGGATTAGCATTAATATTAGGGCTTAATTTATTCTATGGAAAATTAGAAGCACAAATATCAATTGCTGTTTTAATGCCTTTCTTTTCGATATTAGGTGATTTATACTTTTCATATGTTAAAAGAATAAATGCGATAAAAGATTATTCAAAAATTTTAATAAGTCATGGTGGTATTTTAGATCGTTTTGATAGTGTTGCTTTAGGAGGATTAACTTTTCTTTTGTTGTTTGTTATATTTATATAAATCAATTTTTATTTTAAATATATAATTTAATAAATGTTTGGAGGTAATTATGGCAAAAGTTAGAAATGCTGTTATTTTTGCAGCAGGTAAGGGGACTAGAATGACCCCATTAACTCATTATGTTCCTAAACCATTAATAACTGTAAATGATGAATCAATGATTGAAAGAAACATAAAGCATTTACAAGAAATAGGCATTAATGACATCACTGTGGTTGTTGGTTATATGAAAGACCAATTTAATTTAATTAAAGAAAAATATAATGTCAAATTAATTGAAAATGATTTATATGATGAAGCAAACAACATTTATTCTTATGTTGTTTCCAAAAATGCATTTGGAGACACTTTATATATTGAAGGTGATTTGTATATTGAAAAAAATATTTTTCCAGAAATAATTAAAATAATTGAAAACTCTGAAGATTCAATTTGTTTTACTGAAAAATGTACAAAACACAAATCGGAATGAGTTTTTGATACAAATAATAAAGGATATGTAGTTAAACATCACAATGAAAAAGATGCATATAATCAAAATATCTGAATTGGTATTTTATATTTAAATAAAAAATTAGCTAATGAAGCAAAAGAAAAAGTTGATGCATGATTTAATCAACAAGGAAATAAGCAACAATATTTTGAAACATTTTTATGAACTTTAGAAAACAAAATGAGATTGGAAGCAATTTCTTCAAATTTGATTAAAGAACTTGACAATTTTCAAGACTTAATTAATATTGATCATCATTATGCAAACCACGCTTCTACTTTATTATTTACACCAGGTCCTATTAATAATTATCCAGAAGTTTCTGAGATTTTATCTGAGTCAGTGTTGCATCATAGAAGTGAGTTGTTCAAACACTACATGGAAGAATCAACTAATTTAATTAAAGAATTCTTTAGAACTAAAGAAGCACTTCCATTATTTATTACATGTTCAGCAACTGGGGCTATGGAAGCAGTTGTTGTTAACCTTATTAGTCCTGAAAACAAAGTTTTATTGGTTGAAGCAGGTGACTTTGGTAAAAGATTCCAAATAATTTTAGAAAGATTAATTGGAGCTAATAATCTTGATGTTATTCATTATGAAGATGGTGAAGCATTCAAAGTAAAAGATATAGAAAATAAACTTAAAGAAAATAAATATGATGCATTCTTCTGTACTCATCATGAAACATCAACAGGTGTTTTAAATGATATTGAAGCTATTTCAAAAGTAGTTAAAAAATATGCTAAAGATACATTATTTATTGTGGATACAGTTAGTTCATTTATTCATGAAAATGTTGAGTTTGATAATTGAGGTTTAGATGTTGCTATAGCAACAAGCGGAAAAGCATTTTGTGTAATGCCTGGTTTAAGTTGTGTTGTTTTATCTAAAAGAGCTCAACAAGTAGTTAAAGCAAATAAAAACTTTAAATTCTATTTTGATTTATCAGCTTATATTAATTTTTATGAAAGAGAAAAATCAACTCCTTATACTCAAGCTTCAGGTATTTTATTAGCTTTGAATGCTGCAATGAAAGTTATCAAAAACCAAACATTTAAGAATATAAGAAATAATAAACTCAAAATATATGACTACATTAAAAAAGAATTAATTGCTATTGGATTTAAGGATGTAGTTCCTAAAAAGAATATAACTATTGGACTTTTAGTTTTAAATGTTCCAGAAGGTGTTGATGCAATTAAGTTAAGAAATAAAATTGAATATAATCACAATATTTATTTTGAATTAGGTCGTGGAAATAGAAGAAGTAAACAAGTCAGAATTGGAATTCCTAATACTATAGACATGGAAAAAGCTAAAAAACTTGTTAAAGTAATTAAGGAAACTTTAAAGATTAAAATTGATTAAAAGCACTATAAAACAATAAAATAAGTCAAAGTTTGGCTTATTTTTTTATACAAAAAACTTCCTAAATAGGAAGTTTAATTGTTTGGATTATTTTTCAATTTTAACTTTAACACCAGGACCCATTGTTGATGTTACAACTAAGTTTTGCATGTAAGCACCTTTAACTGTAGCTGGTTTAAGTTTTTTAATTAAATTAATGATTGTTTGAGCATTTTCTGCTAATTGGTCTGTTGTCATATTTAATTTACCAATGATTGTATGTACAATACCTGCTTTATCTGTACGGTAGTTAGCTTTACCTTTTTTAAGTTCAGCAACTGTTTTATCAGGAGTAGGTGTAACTGTACCTGTTTTAGGGTTTGGCATTAAACCTTTAGGTCCAAGTTTTTTACCATATTTACCTAATAAAGGCATCATTGAAGGGTCAGCAACCATAACATCAAAGTCAAATTCGTCTTCTTTAATTTTTTGTTCAAGTGCTGGACCATCAACAACGATGTCTGCACCAGCAGCTTTTGATGATTTTTGAATTGCTGGATTGTTTGAAACAACTAATACACGAACGCTTTTACCTGTACCGTTAGGCAACAATACTGATCCACGCAATTGTTGGTCAGCTTTTCTAACGTCAAGGTTTAATTTGAATACTAATTCTACTGTAGGAACAAATTTTGTAAATGATGTTCTTTTAACAATTTCTAATGCTTCTTTTAAATCATATGCAACTGTTTTGTCAAATGATTCACGAGCATTTCTTATTTTCTTTCCACCTTTAAGAGACATTATTCTTCTCCTTCTTTAGCAGCAGCTTTTTCGTCTGAAATTGTTTTAACATCAATGGTTTTACCTTTAGATTCTTTTAGTTCGTTTTGAGCTTCATCTAAAGCGGCTTCTTTAGCTGCAGCAGCTGCGTTGTCTTTTGCAGCTTTAGCAGCAGCAGCTTTTTTAGCAGCAACGTTATCAAATCCTTCGATAATAATACCCATTTGTCTTGCTGTTCCAGCAATAGTTCTCATAGCAGCTTCAACATCATTTTTGTTTAAGTCTGGTAATTTGTATTCAGCAATTTCTCTCAATTGTTCTTTTGTAATAGTAGCAGCAATTGTTGTTTTTGAGTTTGAACTACCTTTGTTTAATTTAGCAGCTTGCTTTAATTTGTACGAAGCTGGAGCTGTAAATAACTTGTAGTCAAATGATTTATCTTTGTAAACGGTAATTTCAACAGGAACTGGTTCATTACCTCTATCACGTGTTGCGTCATTAAAAGCACGTGTGAAATCAGGCATATTGATTCCAACACCAGCTAAAGCTGGACCAGGTTTGGCTTGGCCAGCGATGAATTGTAGCTTACGAACACGGACAATATCAGCTTTTGATTTTGCCATTGTAATATCCTTTCGATAAGTGTGGTACTAATGATACTTTTTGTTTTCTCTCCCACTAGTTAGAGGCAATTATTTATAATTGCTTTTTTATTATATACAAAAGTTTAAAAAATGAAAACAATTAATTTTGCATATTTAACCTTTTAGAAATGAAATAATATTTTGTATAATTTTATTGTAAATAATAAGGAGAAACTAAATGAATAAAAAAAGTTTTAAATCAACTTTAACTTCAATTGCTATAGCAGCAATTTTATTATTAACACTAACTATCTCATTTTTTGGATTACAAAAAAATATCCCAGAAGCAGTAATAAAAGCGACAAATAACAATTATGTGGCTGGAATTACTAACATTATTTTATTAGTTATTACTCTTGCTCTATGCATTTCTTTTATTGTTGTTGGTTCAATTCAAGCAGCAAAATTAAGTTTTCTTAAAAAAGATCGTTACATTTATGTTTTAGCAATTTTAGGAATTTTCTTTGTCCCTGTTTTCTATGTAGTAGCAGGAATTATTCTTGCTAAAAGAGATTCTATTGTTAATGAATTAGTTGATGAAGAAAATGAAGCAATTGAAGTTTAAAAAGTGCATTATTGCATTTTTTTATTCAACTTTTTTATAAAAAAGTCACCAAAAGAGTATTTGACAACTGTCATGAAATGAATGCACTGTTCTTGAATACTTACTTTAAGCAACTAATTATATAGTATGACAAAAAATGTTAAGTTATTCAATAGCAACAAATGTTATAAAAGTGAGATCCACCATTCAAAAAATCTTATATTTATTTGTTTTCATTTCTATTGAAATTGCACTTTTTACTCATTGTTTTTGTAAAAATATTTTGAGAAAAAAACAATTGATATATAATAAATGCACTTAAGCACCGTTAGCTCAGTCGGTAGAGCAGCTGGCTCTTAACCAGCGGGTCACAGGTTCGAACCCTGCACGGTGTACCAAATCATTTATTTGACCAAAAGCACTGTTTTATAGTGTTATTAAAAAATTAGCAACAATCAAATTGCTAATTTTTTTATCATTATTCTTCGTCTTCTTCATCATGAATGTCTTCTGACATTTCACTGTTTTCACCAAACATTTCTGATTTTGCTTCTTTAAGAGCAGCAGTAGCTTTTTTGCTTTTCTCATCTTCTTTTGTACCCTTAACATTAAATTTAATTTTAGAAGTTGCAAATGCTGGTTCATTTTCTTCATCTGTAAAAAGAATAGTTGCCACAACAATTGGTCTTCTTCTTTTTTCTTTGTAGAAAAGATTTTCAAGACGATCAATAATCAATTGTTTTAAATCTACAATATTTCAATCAGGTGTATTTTTAATATAGAAAAGAGCAGCACCATGAGCAATTCTTTTAGCTTCTTCAACTAATTCAAGCGAAGTTTTTACATAGAAGCTCCCACGACTAATTAAACTTGGACGGCCAACTATTGAATTAGTTTTTTTGTTAATATTTAGGATCATATTTACAAAACCGCTATCAGCTAATTTAGCTCTTTCCTTAAGTATATTTGCACTTAAATTCAAGGTTGTGTGTCCATCAATATAAATAGGACCACAATCTATTTTTTCATCTGTTTGTGTAATTTCTTCATTAATCATGTTGTAAACACGACCAATTTCAGGAATCATTATATTTTCTGGTTTAACTCCATTTCTAATAGCGCTTTGGCCATGAACTATACTCATTCTGAATTCACCATGGTAAGGTAAAAAGTATTTTGGTTTAGTAAGTTGGAAAATTTTATCATGTTCGTGTTTGTAAGCATGACCTGAGGTATGCAAGTAACCATCAACACCATTTTCTTTAATAATAGCTCCAAGTTTTGAAAGTCTATTAACTAAAAGTTCAATAACCATTCTATTCCCTGGAATTGGACTTGATGAAAAGATAATCATGTCACCTTTTTCAATTTTTACGCTTTGGTGTTTACCATAAGACATTCTTGAAAGAGCAGCCAATTGTTCACCTTGTGAACCTGTTGTAAGAATAACAAGATCTTTGTCAGGAACTTTTGATACATCTTTTTTATCAACAAAAACACTTGCAGGCGCTTTAATGTAACCTAATTTACGTCCAATTTTGATTCCTTGAACCATTGAACGACCAAAAGCTGCAACTTTTTTGCCAAGTTTAACAGCTAACTCAATAATTGCTGTAACTCTAGTTAAGTTTGAAGCAAAAGCTGTAATAATAGTTTTTCTTGTAGCTTGACGCATGTATTTTTCAATATCTGTTAAGATGTCATTTTCACTAGGCGAGTGGAAAGGACGCATAGCATTAGTTGAGTCTGACAAAAGAACAGTTAGGCCTTCTTTTCCAATTTGATCTAAACGAGCAAAATCAGTATAAATTTCACCAATAGGGTTGTAATCAAATCTAAAGTCACCGGTACACATTATTGAACCATTTGGAGTTTTGATTCTAATACCAAAAGCATCTGGGATTGAGTGTTGAGCTGTTCAAAAATCTACTGAACATTCGCCAAAAGTGTGTACAGCACTTTTTTCAATTTCAATAAATTCAACCTTGTTTTTAATTTTATTTTCATCGAATTTTAATTTCAAATATTGAATAGCAATTCTAGGTGCAAAAATCTTTTTAACTGCTACTTGTTTCATTAAATGAATAACACCACCAATGTGGTCTTCGTGACCATGAGTAATAAAAAGACCTTCAATATTTTTATATCTTTCTGCCAAATATGTATAGTCAGGAATAATACCTTTAATACCAGTTAAAGCTGTATCAGCAAATTTGATACCTGCATCAATAATAAATATTTGGTTTTTGTATTCAACTATTAGTGCAGCTTTACCAATTTCTTGAACTCCACCAATAGGTATTAATCTTGTAGGATTCATGTTTTGATCTCCTTTTTTAATGTTTAAAAAAACTGCTAAAAGTACCTTTATGCTTCTATTATGAAGGATTATTATAAAGATGTAGAAATACAATACAGTTTACTATTAATAGTTATGTAAATATAATACCAAAGAATAAGCAAACTATTATTAAAAAGTATAAAAAAATTATTTGAAATTTTACATTCTGCCAATAGTCTTATCAAATAGTATTATTTGTCAAAAATTTATAATTTTTGGACAAACTAGAATAATTTTTGTTTAAAAAAGCATATAAATTATTTTTCAATTTAATTATTAATACAATTAATAAGGTATAATTTTTTTACTTAATATAAGGAGGTGGTGATGTCACAAAATAATAATTCTTCTTCTTTTACAAATCACCAATGTATTAGAAATGGAAACATCCAAAATAAAGTTCAAAATGAAGAACATATTATTGAAACTATAGAGCTTTATTCAACTGAAGACATGAAAAATTCTAATGCTTTCAAGTTATATAAAAAATCACTTGATTTTGCAATGAGTAACAACAATGACAAAGTGAATAATATTGCTATTATAGGTTCTCAAGATGGACAAAATCAAAATGTTATTGAAACATATAAATAAAGTAGAAAAGATTTTAATATAGCTAATGTTTCATTAGCAAACACAAATAATGAATTATTAGAATCAGAAATCATTAATCAAATTTCAGGCCAAGTTACTAAAAAGAATGTTAATCAAGCAAATAATAAAACAAGTGTAAAACATGTTGTATTATGAACAGCTTTAACAAGTATTTTCATTTTATTATCAGTAGTATTTTTTAGCTTTAGTGCAAGAAAAATTATTGCTCATGGTTGAAGCAAATCATTTGATTTAAACCATTTAGGATTCTTCTTACCAGTTGCATTTATTTTATTTGGAATGATAGTTGGATTCTTAGCAATTGCTATTTATTATGGTAGAAACTATTTATTAGACTTAAAAAAATCAAAAAATATAGTAGTTCAAGAAACTAAAGAAATAGTAGTTATTGAAGATAAAGAAAAACTTGTTAACTTAATTGACAAATCTGGCTTAGATGCTTTGATTATAGAAGATTTAGGCGAATCTAAAGATTATCAATTAATCAAAAGAATTAAAGATATTAACACACTTGTAAACAAAAGAAGACAAAAAAGAATTGAAAAAATTTTAGCTAAACTTGAAAATAAAAAAGAACAAATTGCTAAATTTTATGCATCAAAATACTATGTTAAAAAATGTAAAGCAATTAAATTCTTCTATGTAATTGACAACACACTTGCATCACTAGAAGACAAAGGTAATATTTTTGATTTTTCATTAACTGTTTTACCAATTAGCAAAGAATACAAAAAAGAACAAATCAATAAATTGTTATCACAATCAAATTTAAGTGTTAAAGAATTAAGCTGAATAAAACAAATTGAAGACGTTAAATTAAACAAAGTATTAATTAATGATTTTAATATGTACTCAGATATCCATAACTTCAGAAGCACTAACACAATGGGTGATGATATTGCAGAAGAATTCAAAAAAGATTACAACAATAAATTTGAAGAAATTGTTAAATATGTAAACAGTTTATTGCAAAATAATGTATTTAGCAAAATCAAAAATCAAAACAATTTAGCTTTAAATGCATTCAAAGCTTTAAAACAAACATTAGGTGAAAACAACAAGATAGCTCAAGAATTAGCTTATGCAGGTTGTTCAATTCACTATCAAGAAATTGGTTTTGATCCTTACATTGAAAGTCAATTAGATTTATTAAGAAATAATTCAGATCTTGAAATATTCTTTAAAACAAAAAAACCTAATGAAAGTAGATATTTAATTACTTATGTTCGTAAAGCATTGAAAAACAATAGAAGTAAACTATTTGCATTCTTAATTTACAAAAACTTATTCTCACATGAATACAGCAACCTATTAGAAGAAAAAGGTTGATTATACAAACTTAATTCAATTAAAAAACAACAATACAATAGATTATTTAAAATAGTGGAAAATGAAAGCAAAGAAAAAGTATTTGCCAACATTAAATTAGATGGTAAAGCAATTACAGTTAAATCACTTGCATATTTATTAACCTTTAATTTATGTTCACAATTAAACTTAGATATGAATTTAACTGAATCGTTCTACTGATCAAGAGAATTTGTAGCAAATGCTATTGTTGATACATTAACTAATAAAGAAAATTCAGAAGTTGTAATAAATAGAGAAATCTTAATGGACTTCACACAAGATGAATACAACAAAGTTGAATCTAATAAAAATATCATTAATTTTGAAAAAGCAAGAGAAGAAATTACTGAGAAATATCAAATTGAACATCTTGAATTAATTGAGCAATTATTCAAAGAATCATACTTAGGTTTAGACTGTCAATATTACATGAATGATAGTCATCAACTTTCAAGTGAAATCAAATTAGATAATTCAGATGATGATGAAAATGGTGGCGAAAAAGAAGTTGACTTAAATGTCGAAGTTCAAAATGTTGATAATCTTGAACAATTACAAGCAGAAGCTCAAAAAGTTGTTGATTTAGAAAACTTGGCTAACTTTGAAGAAGCTGAAATTGAAGAAAAGAAAAATCAAAAGGTTGAAACTCAAAAGATAGAAGAAGATGCAAAAGAAACAATTACAGAAATTAAGGAAGAAGTTGTTCCTGAAACTAAAGTTGAAGAACAAAATATTAAAGTTGAAGAAGTTAAAAATCCTTCTGCACAACAAGCTCAACCAAATCAACCTAAATTCAATTTAAATAAACCTCAAGATCAATACCAACAAACTTCTAGACTTCGCCAAGTAAATCCAAATATGTATCAAAATGTTCAATATCCAAACCAATATCAAAACTTTCCAAATAAAGGCTATGTTGATCCAAGATTTGCACAATATCCTCCTAGACAAAACCCATATTATCCAAACCAAAATTATACAAACATAAATGTAGCTCAAAGACAACCACAAAATCCTAATAATCCTATAAAACAATAGTTTTCGTAAAAAAATAGAACTGAGTTTTAATCTCTCAGTTCTATTTTTATTCATTATTTATTCAACATTTTCGGCATTAAAATAAAGTTCATTTAGACTTAATTTGTTATCAGCTAAATTATAAATTTGAATATTGTTTTTTAAACAATATTGAAGAAATTTATTAACTTGTTCTTCATCTAATTTTGCATTAATTCAATCATGTTTTTCAATAAAATCAATTCGGTTTTTAATTAAATATTCCACTATTTTATTGTTATCATTTGTATTTATTTTTTTGTTATATTTTGCATTGTGCAATTTTTCTTTTATTGTTCCATTGTCTAGTACTTTGCCCTTAGCAAGCACCGTAAAAGAATCGATATATCTTTCAAGTTCTAGAAGAATATGACTAGAAATAAATATAGTTTTTCCTTGTAAACTTAATTCTTTTATTTGTTCATAAAATATCACTCTAGCACTTGGGTCTAAATTAGCAGCAGGTTCATCCATAATTAAAATTTCAGGATCATTTAATAAAGCTTGCATTAATAAAATTGTTTTCTTTTGGCCTGATGAAAGTTGCTCTGCTGATTTTTCAAATTCAGGTCCATCAAAGTGATATTTTTTTAATAAGAAATTTACTTTTTCTTTTGCTTCTTCAGGTTTTAAATTTGACATTTCAGCAAATGCTAAAAGATATTCTTTTACTTTTACTTTTCTAGGAAAAAGAGAAACTTCAGGAATATAACCAATAAATGATTTTGCTTTCTTGTTTGTTGAATCAATACCATTGATAAATATTTTGCCTATTTTAGAAGGATAGAATCCCAGTATTGATCTTATAGTTGTGGTTTTACCGGCACCATTATCACCAATAAAACCATGAAATGATCCTTTTTTAACATCAAAACAAACATCGTCTAAAGCAGTTTTAAAATCGCCATGTCCAAGTTTAAAAAATTTATGTAAATGTTGAATTGAAATTGCAACATCCTCTTGAGGCTGTTGATATTCATAATTTTTAGCTCTCTTGATTGTAGCTTTATAATTTGCTCTTTTTTCAAGATAAATTCTTCATTTACTTTTTTTAACTAGTGTTTTCATTATTTAAAATTCTTTCTAACAAATATTGCTCAACCACCAGCCATCATAGCAAAAGCAGCTAACATTGATAAAATTATTGATCCGCCAACTGTCATGTGATCTTTTCTTGTAACAACAATTAAATCTCTTTCAAGTGGTGTTAATTTAATTAATTCAATACCACCTGCATTGTCTAATTCATTTAATTCATCCAAATTATTAGGTGAAGCTTGATAACTTAAAACATATTGTTCTTTATTTAAACCATACATAAGAGTCATTAAGTAACCAACTTTTTTAAAAAGTTTTTTGTTTTCTTCTAATTTTTCTTGATATATTTTAAGTTTCTTTTCATATCTAATTCTTTCACCAATTGAATTAGATTTTGGTTTTTTAGGTTGTATTGTGAGTAGCTCTGCAACAGCAACAAAAAATTCATTAAGTTTTGCTACTTTTTCATCATTAAATTGTTCAAAGAAATCGATTCATTGTGGATAATATGTTGCAATATACGTTTTAATTTTTAAGTTTTTGTATTTATCTTTTTGATGTTCTTCAACATAGCTTAATATTTTGTTAAAGTCTTGAAATACTTTTGATAAGACTAATACCTTTTTGCCCTCGTAATCTATAACATAAGGTAATTTGTTAAATTTGATCTCTTGAATATTTTCATATAATTTATCTAAAAATATTGATTCAAAAAGTTTTAACTTATCAATCATTGGTTCAATTGTTTCTTCTTTATAAGTTTTATAAAAACTTTCATATAAATCTTCTTTAGTTAAGTAGCCTTTACTTAATTTATTTTTAATTGAATTTGCATCATAACTAACACCAAAAAGATTTTTTGATTTAACTTCTTTTATAGAATTTTTATTGTTATCTTGATAAACAACATTTCAGATATTTAATTTAATATTGTTGTCTAAATGATAGCCATATTTTTCATACATTCGATAATCTATAGTACCGTCACTTTTAAGTGGATTTATTTTGTAATAACTAATTTTTTTGTTAAATGAATTAATACGATGATCAAAGCAACCGCTTTGTAATGAGAAATAGTTTTCAATGTTTAATCAATATAAATAGTTATAAACTGACTTATGAGTATTGTCAATATATTTTGTGATATCTTCTGAATCTTCTGTAATATTTAATAAAGTATTTTTTAAGTCATCGTTGTATCTACTCATCAAGATAGGTTGATTTTCATTGAATCTGCCTTTTAATTCTTCAAGGAATTTCTTGTTGCCTTTTTCTTTAGAAGGCAAGGTCGCTATAACATTATTTGTTGATTTAGTTAAGCTTTTAATGTTATCTACAGATCAAGCAAACTTAACAACACCGAAAATAATTGTTATAAGAAAAGGTATAGCACCTATTAAAATTTGAAACATCCTTAAACCCACACTTGAGTGTACTAAGCTAGCAATTCCTAGAGCAAAAAGAGCTGTTAGTGTTATTGCTAATAAGTGAGCACCTGAAGCACCTCAGACCTCTTTTCAAATAATTGTTGGATAAACCAATCTGCTTATAAAATAACCTAATTCGATAATTGCTATTGAAAATAATAGACCAATTGCAGATACAAAAATATTAGCAAAATATATTTGAGTTCTACTTAAAGGTTTTGAATATAAAATGTAATCCACACCTTCTCTTTTGTATTTATAAAATACAGTTTGACACACAAAAGTGGCAACAATAAAAATCATAAAAATTGAAAAATGGTTAATATTTGTTAAGAAGTTCACCGAATATAAAAGAGTTTCAAAAACTTTTGGACTTGCAGCACTTCATTTAAGAACAACATTAATAATGATAAAAAAGATACCGAAAATTAAAGGCACGACATAGCTTGATGCGTGTCTAAAATATATTTTTAGTTGTAAATTAAAAAATGCTTTCATAAATTAAATTATAAATTAATATTAAATTTAATTATTCAAACTTTGTAAGTAACATTTCTAAAGTTTTATATAAACCACCTTTTTTATTTGTAAAAGGACTAATAAAATCTGCTTTATCTTTAAATTCAGGAACAGAGTTTTTCATAGCAATTACATAACCCATATGTCCACTAGCTGTTGAATCATTTTGTGAATCACCAACATGTCAGCAATCTTCAGCATCTACTCCAAAACTTTTAGCTAAGTATTTATTTGAACTACCCTTAGAAGCAAAAAATGATGTAACTTCTAAAAATTCATTTTTAAATCCTGATATAGATACATTAAGATCTTTTGGTCATAATTTTTTAAGTTCTTCAGCAAATAAATGAAGTTTTTTCTTTGACAAATTAAGAAAAAGTAACTTAAATACTTCAGTTTCTTTATTAAATTCTTTTAGTTTGTAGCATTTGCCTTTTTTGAAAATTGAAGCAACTTTAAAAAGAAATGAATTCGAGTACAAACCATTTTTAAAGTTTGAGTTAATAATAATTGTCATTTTTCATTTTTCAGCTAATTCAAAAATTTGTTCTACTACTTCTTGATCTATAGCTGCTGAAAAAATTTCTTGACCATCTTTTAAAACTTTAGCCCCATTTCAAGCAACAAAATTATTTTCATTGCCACATTTTCTAGCCAACATTTTTGTTTTTTCAACTACTGCGCGGCCAGTTGAAACAAAAATTTCTAAGCCATATTTTTCTTTAAGTTTAGCTAAACCTTTTTGGTTAATTTCACTGATATCTTTTTTAAATTTGCTACTTTTATCAATTGTTGTACCATCCAAATCGATAAAAATTATTTTAGGTTTTTTTAATTCTTTAGTCATAATGCTTTTATTATATAAAAGATATTAAATTTTGATTATTAAAAACACAAATAAAAGAATGTACAAAATATTTTTATTTTTGTTATACTTTTAACATTAAAAAAGGACTTAAATGATTTTAAACTCAGTATTTTATAAAGCAAATAACCCATTTTACGAACAAGGAACACACAAATTAAATGCTCCTTATTTAGCTTTATTTATTATTGGATTATCACTAATAGTAATTGGAATTACATGCTTTTTCTTTTATCCTAAAGCAAAAGACAAGGTTTATTTGTATAAAGAAAAACAAATGGAAGAATATAAAAAAAATAATCCAAAATCTAAAGTAACGAATTATGAAGCAACAGGAATGTATTTACCAGCTTGAGAAAGAATTAAATTATTTGCACCATTATTTTTCGGGATCTTATTTGTTGTTGTTGGTATAACAATGATTGTTGGCAAAACTATTTCGACATTATAAAGTAGCTTAGGCTGCTTTTTATTATATTTTTTAATTTATTTAACTATAATAATAGCATGAGTAATATTATTGAAATGAAAGTTGAAATTCAAAAGAATTCAAACATAAAATACGAATATGATCGTGCTGACGGAAATATTCATGTAGACCGTATTTTGAGAGGAGATTTTGTCTACCCATGTAATTATGGCTTTATTCCTACAGCTTTAGATTGAGATGGCGATGAGTTAGATGTTTTACTTTACTCTCCAGAAACATTTACACCAGGTGTTGTTCTTAATGCAAGAATTATTGGTGCTATGAAAATGATTGACTCAGGAGAAACAGATACAAAATTAATTGCTGTTCATGCTGATGATTATCGTTTAGATGGTATTAAAAAATTAGAAGACTTACCTGAACCATTTTTAATGCACATTGAAACATTCTTTAGCAACTATAAAAATTGAAAGAAAAAAGGAGCTACAAAAGTTCAAGGATTTGAAGGCGAAAAATGAGCCTTAGCAGAATTAAAAGAATGTGAAGAATTAATGGTTAAATATGGCAAGATGCCTAAAAAAGAATTCATCAAGAAAATGATGAAAGAACACCCAGAAAAATATGAAGCTTAATTTTCCAAAACGCCTTTAAAACAGGCGTTTTAGTTTATTTTTGTTGATTTCAAAGTCTTGAATTTTAAATATTATTTATAAATTTTAATATAATTAAAATAGGAGGATTGATGAAGAAAAAAATATTAATGTCATTTGGTCCAATTATATGTTTATTACTTATGTCATCATTTTCTTCTTGAAGCTGCAATAATTCAGCTGAACCACAAAAACCTTCAAACGATGATGACAACTGAGAAGATTATAAACAGCCAAGTTTAAGTGTTCCTAATTTTGTGCCTGAAGGTGAATGAGAAGATGATGGCAGTATTTACAAATATTATTTAAAACCAAATACTGCTCAAGAAATTGAAATCAATTCTAAAGAAAAAGAATTATCTTTATTAAAAATAAAGAAATTTGTTTCAGATATTGAGATAACTAATTTTGAAGCTAGCTCAGATCCTGAAGTTAATAAAATTATTAAAGATGCTTGAGCAATTAAACAAGAATTATTGCAATTAAACAATGATGTTTATGATAAATTTGGTTTGCATGAAAACCGTTATAAAAAATATTTAGTTGCTTACAAAAATTTTATAAATTTATATATTGATAATGAAAATAGCAGAGCTTATATTTTTGATCTTTTAGCTCCAAAACATAATAATTTTTGTTCCTGAATTTCATTTTATTTAGACAAATACAAAAGAGGTGCTTTTGATCGAATAGTCAATAATAAAGATTTATTCTACAAAAATTATGACTTGTTTAGAAACTTTATTGATAGAAACAAAAAGGATCTTGAATTAGTTTTTGATTTAAAAGATGCTAAACAAATTAATATTAATAATTTAAAAATGTATACAGAAAATATTAACTCAGTTTTAAACAAACAATTGAATGTTGAACATTTTAGAAAATCATTAGTTAAAAATGGTGTTAATGTTGCTCCTCTTATCAAATATTATTATGGCACGGATAAATATAACAGCTTAAGAAATTTAGTTTATGATCTAACGGGCGGTTCAAACTATTTTAATGATTTTTGTTTTAAAGGAAACCACTATAAAAATTCAACATCTGTTACATTTTATCAACCAGAAAATAATGTAATAAACATTAAAATAAATCGTTTAGCTTATAGAACTGAAAATGCACAAGAAGAAATTGATATCAATAAATATTTTGAAAAAATTATTTATTCTATTATTTCAAAACAAATGAATGATGAACAAAAAATTAGAGCTATTCACAATTGAATCATTTTAAATAATGACTACTTAAGAGATGATGAGATGGTATATTGTGACAAACAATGAGCTCAAACTTGTCGTAGTCCATATGCTTATATTGGAAATTGAAGAATAGTTTGTGACGCTTATGCTAGAACATTTCAAAGATTTATGACTCTTTTAGATATTCCATCTTGATATATAACTGGACCAGTTAAAACTTATGTTGATGGCAAACTAAAAGATGATGAGGGCCATGCTTGAAACCTAGTTTTATTAAATGGTAAAGAATATTTTGTAGATTGTACTTGAGATGATCCTTCTTATAATGAATATAAAGAAAAATACAATGTAAATTCAAAAGAAATTAGTCCAATTAATAATAACTATTTATTAAAAGAATGAAAAGATATTAAATATGAAACATATATTAATAAAAATAATAAAAAGATAGAAATAACCAGAGAACTATATGATAGTTATCATGATTTATATGAATTAAGAACTAACTTAGGTCTTCCTTATTTTAAAGCTAATGAATATGTTTAATAAAATTTGTAGCCTTATGGTTGCATTTTTATTGTTCACTTTTAAGATATTTAATTAAAATTTTGTTATTATTTAATTGTAGAAAGAGATAAATATGAAAAAAAATAAAATATTTAATTGACTAGGTACTCTAACTGCTATTGTTAGTCTACCAACTATAACAACATCATGTTTTTGAGATGATGTTACTGGCAATATACCTTCAACACCAAGTACGACAGTTACACCTGTAACACCAGAACCAGGTCCAGGCCCAGCCCCAAAACCTGGAGATAATAAACCATCTAGCATCTATAAATATGGATTAAAAGAAAATACAAAAGAAGAAAAAGAAGCAAATGAAGTTGAAAAAGCTTTATATTTATCAAAGGTAAAATATTTTGTTAGTGATGTAGAAACTGTAAAAGATACAATAAGTTCCAAAGTTGACTTGTTAGTAAAGAAAGCTTTAAAACTTAAAAAAGAATTATTAGAATTAAATGAAAAAATTTTTAATGCTTTTGGATTACATGAGGGAAGATTTGAAAGTTATTTAAATGCTTACAAAAATTTCGTTAATATAGGTTTTGTACAATCAAATACAGGATTAACTGCTACAATAAATGGGAATCCTGAACCTACAATAAAATATATTTTAGAGAATTATATATCTCAAGGAAGTCTTCTTAATCTTAATTATAAATTTTTTAACGACATTTTAAGCAATATAGAAAAATCTGTAAAGTACTATAAAAAAGACTTAGACTTATCATTTGACATTAAAGATGCATATGAAGTTGATTTAAACAATTTGGACTTTCTAAAAAGTGCTAAAGCTGTTGATACTCTCACAAAAGAACAAGTTTTAAAAATTAGAAAAGACTTGCTATTTAAAGGAAAAATAACTTCTGATATTGCCGTAACTAATCATAATTTAGGAGATTATTTCTGAGATTTAAATTCAACTTGATATGACATAACTGGTGGTAGCAATTATTTTAATGACAAAATCCTTGAACGTATTACTTACACGCGTCAAAAAATAAATATAAAAGAATCAGAATTTAGATATAGAACAGATAGTGCTTCTGAAGAAGTTAAACTAAATTCATTGATGACAGAAGTAATTTATAAAATAATTTCTAAAAAAATGACTGATGAACAAAAAATAAGAGCAATACATAATTGAGTGATTGACAATAATGAATATTTGACTGATGGAGAGTTAGCTTTATCAAATAATGAACCAATACGAAGTCAATGTCGTAGTCCATATTCATATTTTACTAAACCAAAAAGAAGAATAGTTTGCGAAGGATATGCAAGAACATTTTCAAGATTTCTAACATTAATAAATATTCCTGTGTGATATGTAACCGGTCAAGCTGCTAATTAAAGTGGGCAAGAAGGTCATGCTTGAAATATGGTAAGACTTGGAGGGCGAGAATTATATTTAGATCCAACTTGAAATGATCCAACAATGGGCCCTTCAATGAAAAATTATTTTGAAGGTAAAAAAATGATGCCACATAGATTTACTTATTATTTATTAACTTGAGATCAAATAAAAAATACTGGAAATACTGCAAGAGATTTTGATCAAAGTATTAAAAAAATTGCTGCATATCGTCAAAGTTTAGGTTTAGATTACATACATGTAAATTAATTTATGAAATAAAATATTTTCAAAAAACTAATTTCTTTATATAATATAAAAAGAATTTTGAACTTTTAGGAGGATTAATATGCCAAGAGACGGTTTTACATTAGTTTGCGAATCATGTAAAATGGAAAACTACATTAGTAAAAAGAACAAGAAAAATACACCAGAAAAAGTTGAATTAAGCAAATATTGTTCAAAATGTAGAAAACACCAAAATCACAAAGAAAAGAAATAAAAAATAATATATAAGAAGCTTGCTAAATTAATAAGCAAGTTTTTTAATTTAAAAAACACCAAAAATTTAAAAAAGTTAGTTTTTCAACCTTTTTACTTGCCAAAAATTAACTTTTTAATATAATTAAAATTATGAAAGAACTAGAAAAAATTACTCCATTAGAACAAGATTTTGCTAAGTGATATACAGATGTTGTTAAACAAGGGAATTTAATTGCTTATGGGCCTGTAAAAGGAACAATTGTATTTAAACCCAATTCATATGGAATTTGAGAACAAATTCAAAAAAATTTAAATGAATGTTTTAGAGAAAAAGGTGTGCAAAATGTATATTTGCCACTTTTAATACCTGAAAAATTGTTTAACAAAGAAAAAGATCACATAGCAGGATTTAATCCAGAATTAGCAACAATTACTAAAGTTGGCGACAAGGATTTAGATGAAAAAGTATTTATTAGACCAACATCAGAAGTTTTATTTGCTGATTTATTTAAAAACTCAATTGAGTCTTACAAAGACTTACCTATGATTTATAACCAATGAGCTAATGTTGTTAGATGAGAAAAAACAACCAACCCATTTTTAAGAAGTAGAGAATTCTTATGACAAGAAGGACATACATGTCATAGTAATCCGCTTGAAGCTAGAAAATTTACAAAAGGAATGATTTCAACTTATTCAAAATTTTTAAAAAACTTTTTAGCAATACCAACAATTATTGGTAAGAAAACGCCAAAAGAAAAATTTGCTGGCGCATGTTCAACTTATACAGTTGAAGCAATGATGAAGGATGGTCGTGCTTTACAAGCAGGAACAAGCCATTATTTAGCTCAAAACTTTTCAAAACAATTTGAAATTCAATTCAAAACTGAAAACAATGAATTAGATTATGCTTACCAAACTTCATGGGGTGTTTCAACCCGTCTGCTTGGAGCTATTATAATGACTCATGGAGACAATAGAGGAATTATTATTCCACCTCGAGTAGCTCCAATTCAAATAGATGTTTTAGAGCTTTTTGCAAATAAAAATAAAGAAGTTCATACATACTGTGATAATCTTTACAAAGACCTAAGTAGAAAATTTAGAGTAAGACTTGATTCAAGTGACAAAGGTCCAGGATTTAAAGCCTCAAATTCTGAAATTCAAGGTGTTCCTCTTAGAATTGAAGTTGGACCTAAAGACATTGAAAATAAAAGTGTGACAATTGTTAGAAGAGACACCCTTGAAAAGATTAATATAAAACAATCAGAAGTTAAGGATCAAGTTGAAACTCTTTTAAATCAAATCCACGATAACTTGTACTTACAAGCTGAAAAAAGACTAAATGAAAATACAGTCTTTGTAGATAACTACGAAGATTTCAAAAAACAAATTAAAAATCATAAATTTGTTATAGCCCCATTTTGTTGTTTAGATGTGGCTGAAGAAATAATCAAAAATGAAACTGGCGCTTCAACTCGTTGCTTACCTAAAAAGTTTGATAAACCAAATCAAAAACACAAATGTATTTTTGAAAAATGTTGTAAAGAAACCAATAGATATGTAATCTTTGCAAGAGCTTACTAGCAATATACTTTTATTTACCTGATTTAAAAAAGAAAGGTAATATGACAAGTACTATTTCGCAATTTGCTTCACAATACCAAATGAGTAATTTACAAAAGTATCGTAGTGTAGAAACAATAATTAATATGGAAAAAGCCATATTCTTGAAAAATCGTCAAGAAGATTTTATAGAAAAATATTACAAGAAGAATGAAAATTCAAGATCTAATAATTACTTTTCAGACAAATTCAAAAGAGTATACAATTGCTTAAGCGGTGTAAGTCAACTGATCATTAAAAATGAGTTTATGAAACAATCTGAAAACAAGTTATGATACCAGGATTATTTTTCAAAATCGACATATTATAAAAATAGAAAAAATGCTATTGACGAATTTTTAATTTATTATTTGGACTATACTCCCCGTGCAGTTATAAAACCTTAATATAATTTTAAAATACCACAAAATTAGTGGTATCCTAGATTGTAATCTGTAAGTTTACACCTAGAAATTTGGAATATCGCATGTCGATATTCCTTTTTAATTATTAATATTGTTATTATTTTTTGTCCAATTGGACAAATAAAAGTGAGGGCATTAGCCCTCCCTAAAATATTTGGCGACTTGTCGCCCTTTCTAAAATATTATTTTAATTTTCCTATTTTTTTGAAAAACAAATTTCTATTCTTGTTACTTTCTCAGTTTCTCTTTTGTTTTCCATATAATACTTTGTCAATACCTGTTGAATTTCAAGGATTTTCTGCATTCAATTTTTCGTACATTTCCAAAGCAGATAAATATCCAAAAATTTCTCTTGGACGATTGTTTATTTCATTTTGTACTTTTAGAATTTCTTCTTCAATAATATCATCAAAATTAGTTTTTTTTCTTAAAAAATCTTCTAACTAAACCATTGAAATTTTCATTTGAACCCCTTTGAAAAGAAGCGTATGGATCAGCTTTATATATAAAAATTTTTAGTTTATATCCAATGAAAAATAATGTTGAAAATTCTAATCCATTATCCTGAGTTATAGATTTAACATTTAATTTATGTTTTCAAATAAGATTCCATAAACACAAATTTAAATTTGATGGATTTTTACCAAAAATCCATATCTAGAGACTCTTTCGGTAAAACTAAGGAAATGACAATGTGTGCCTTTACTCTTGCCTACAATAAGGTCTATTTCCCAATGGCCGAATTCTTTTCTTTCGTTTATGTTTGCTGGTCTAGATCAAAAAGGTCTTACATAACGTGCGCCAACCAATCTTCTAGCAGCGCTTTGTTTCTCTCTTTTTCCACCTTTTTTGTAGAATTTTCTTAATAAATCTTTTCAGTTCAAAATTCAATGACCACTTTTTATTCAATTGTAGACTGTTTTAAAAGAAGGAACATCAATATTAGGATAAAGATTTTTTATTTCATTATTAATAGTTGTTCTTGAATAACCTAATATTTTTGCTATTTTTGTTATACTATAATTAGTTGTTCAAAGTAAGTATTCAAGAACATTGCGTTCATTTCATGATAATTTTTTTTGCATTTCTTCATGACAGTCGCCAAATACTCTTTGAGTGACTTTTTTATAAAAAAAATTTCACCCATTATAGATTTTATCTAAAATGTGTAAAGTTTCAGATTACAATGTAGCAAAACAGTTAATATTGTTTTTTATTTTACAATTATTTTCAATGTTAAAAGTTCCTACCTATAAAGATTTAAATATAAATATTAAAAAGATTTTGTGGACTAAAAATTATAAAAGCTTTACAGAGTTCCAAAAGGCATATAGTGGCGTTTTTTGTGCTAAATTAGGTCATTATCTAAGTAAGCATAAAGCCAACACCAAAACAAATTTTTTAAATATGTATCAAATTATTTTTTATCAAGGTTTTATTAATTACAACAGAGAAAAGGAATTATTAGATTTATTGACTTATAAAGAAAAGTCTCAGACATATTAATAGACGCGATTATAGGCATTGATTTAATTGCTACGATAATATTACTTATTTAATACAAGTTAAACCCAGCAATCATTTTATTAATTTAAAGCATATGGGAATAATCACAATTATAAAATTGTATTAGCATTTAAACAATCTAATAAATGAGTATTTAAAACAGAAACTAATCAATTATTACACCTAGTTTAATTTTTCATGTTATAATCTAATTAAGAATATATACAGTTATTAATTTAGGAAGTTAAAGTTAAGGACTATATATATTCTATAATACTTTTAGTGCTGTTTTATAGTAATATTTATTAAAAATATTAATAACAATTTCCCAATGCAGTTATATAAATATCTAATGTTTGTTTTGGATCGTTGTCTTATTATTTATAAATATGAAATGTCGTAGCATCTAAAAACCTTATAATAAAATGGAGAGTGGCATTTTGCTAATATAATTTATGAAATTATCTACTTTACTAAATACAATTAATGTAACTAACATAACACCATCAGTCATTGTTGCATCAAATGAAAATTTAAATTCAGAGCAACAAAAAATTTTGGAATTATTTTCTCGATTTACAAACGATGAAATTGAAATTTTTAACTATATCTCAAATGATATTATCAATAACAAAAATTCAACTAAAAAAGTTATAAATTCCATTGATGAAATAAAATTAGATAAAATTTATGATTTGTTTATATCTAAAATTAGCCAATCTAGTATAAAAAATAAAGATGAATTATTAAATAAACTAAGAGACAAAAGAGAAATTTTTAAAACTGAATTAATTAAAAAAATAAAAGAACAATCTTTAACTTTTAAAAAAAGCGAAAAAAAATCAAAAATAGAAATCAATTCAATGATAAAAAGAGTCAATTCGTCTTTTGAAAATAAAGATTGAAAAATAAATGATGACGGTACAATTTCATCTAAATTTATTTTAAAAGATTATGAGTTATTGAATAATCATTTAATTAACCTTAATGATTCAATTAAAAAAACAATAGATCATATTGCGTTCTTGCAAAAATGAAAAATCGCTTGTGTTTCATTATCTGCCATATCAGCAGCATCAGCAACTGGATTATGAATAGCTAGCTTTTTCTGTCCACCATGTGCAATTGCTGCATCAGTAGCATCAGCGTCAGCATCTTTGCTATCAATTGCCGCAATTACATTATCTGATAGAATATATTATTTAAACGAGACTCTAAAAAATTCTCAAAAATTTATTGAGCTATTTCAATCGTCAAATTATAGCATACCAATATGAGGTATGGTTTCAAGTATTCCTATGGCATTAAATCGTGTTGCTAGAGTATATGAAATGCTTAAATTATCACAAGCTGCGCCATCAATAGCAACTATGGCTACTGGTGTTGTTGGTTCAGCATTACAAACTCTTGGATCATTTTATGATGTTTACACCGCTTCAAAAGAATTAGATTATTTTAATGAATGTGCTAAAAAGGCATCAGATTTAATTAGTGATTTTTCAAAGAAAATAAAAGATATTAAAAAAATAGGATGAGTTGTTATACATGAAAGCGAACAAACTGGTCCTTATTATATGGGAGGTACTGGTGGAAAAAACCTTATTTTTAAAAATTTAGAAACCAACGAAATAAAAACTATTAATGAAATGCTAGAGCTTTCTGATTTTGAATTAAGTTTGTGAAGTTTACAAAGAGTTAATGACCCTAAAAAAGGAACATATTTGCGTAAAAAAGCTAATAATTCTTTATATGATAATTTAGGATAATCACATATGAGTAATTTTTTAATTTTTTCAATTATCTTTTTTAGTTTTTGAATTGCTTTTATTAATCTTACGCCATTCATTATTTTGTTTCCTAAAATTAGATGATATATATATGCTTTTGGTAGTGTAAATAAATATTGCATGAAAAGAAAATTAAGAGTTCAAAATTCATTAGTAAATCATTATTGTCTGATGTCTAAAATTCCATTAATTTATGCTTTACTTGCAATCGCATTAATGCTAGAAAGCTTAGTTTTAATGGTTTTTCTACAAGTATATGAAAAGGAATCATTTTTTACAATGTTTGTAACACTTGGCATAATATTTTATGTACCATTGCCCTTGTGATATATTGTTGTATGCTTACTATGATATATAAAACAAAAAAAGTGAAGAAAGTTTAATAATATGCTTCCAGATAGTTCTTTAATCGAAAAATCAATTGACATAAACACAGATGTTGAACAATTGTATCCATCTAAAAATAAATGTTATTTTAAAAGACAAGGTTTTAATGCACTTTATTTTTCAACTTTTAATACTCAAAAATTCAAGTCATACTCATTCGAAAAGCAAAAACTTTTTATTTATATGACTATGGTTCTCAATTACGATGATACAGCATTTGATAATAAATTTGAGCCGCTAAATATTAATATGTTCAAAAATGAAGCAAAAGCTTATAAAATTATTGAATAATATATTATTCATTGTTTAGAAAGTTAAAAATGATTTGATTAGTGATTATTATATGTTTTATTACACTTATGCCTTTGTTCATAGATTCAATTTTAGTCAAACAAAATACCTTTAAAAAAATCTTATATTTTTGTCTAACAGTAAGACCTAATTGCATAAGTAATGAAATAAAACTTCAAGATTTTGTTATCAAAAACCAACAAAAATATCAATTATTCTATATGTTAGGTTCATTGGGCTGACTGTTAATAGAAATACTTATTGCTTTGCTAGTAGGATTATTATGAAAGCCTAATATCAAATACTATGATTCGCTTGCATACTATTGAATTATATTCGGCGGTAGCACTTTTTTAGTAATTTGTTTTATCCTTATACAGATAAACATATTTGTAAAAACAACCAAATGATTGAAATTCAACAATAATTTAAATGACGAAAATCTTTATGAAAAGAAGATTAATTCAGAAACTAACAAAACACAATTTGATTTTTCATTAGGCCAAAAATATCACTATAACTCCCTTCCAAATGAAAAGTCGTTAGTTTCATTCTTTTTCAGATCAGTCGATTTTTATATTAAATATCCAGAAAATTTTGAAAACTTATCTCCTAAAAAGCAAAGAAAATTAGTATATGCAAATTCAATCTTCGATTTTGAAAATACTAAGCCTGATAAATTAACATATTTTGATATTAATATGTTTAAAGACACATTTATTAAATATCAAATTATCCAGAATAATAAAAATGATTAGCTTTAAAGGCTAATCCCTAGATTGTAATCTGTAAGTTTACACCTAGAAATTTGGAATATCGCATGTCGATATTCCTTTTTAATTATTAATATTGTTATTATTTTTTTCCGATAGGACAAATAAAAGTGAGGGCATTAGCCATCCCTAAAATATTTGGCGACTTGTCGCCCTTTCTAAAATATTCATTTTAAATTCCATCTTTGTAATTTTATAGTCTAAATATTCTTTAAAAAATATAAAAATTTCTATCCATTTTTCAGTATTTAAATGTTTTCCCATAAAACTTCATAGTACAAAAAGACTTAAATCTTTTTGGGATTTAAGTCTAATTCTTTTGTCCTGGTTTAATACCACAAAATTAGTGGTATTTTTCTTGTTAATTTTTAACATAAAAAAGCTTAAAAATAGGCTAAAAAATTATTAAAATAAATTAATTTACATAATTAAATTTTTATAAAAAAATATATGTATAATAGTAATAATATGCCTAAAGAAAATAGAAAAATAGAAAATGAAAATTTCATTACATTTTGTAAAGATATTAATGTAAAAGTGACACCTGGATTGGATAAAGTTGAATTAGACGATGTCGAATATGACAGTGTTAAAGACCAATTAAATGTGTCATTTAAATTTACAAATTCAATCAGTGCAAAGGAATTTTTTTCGTTTCTTTCTAGTTTAAAAAATAATAAAGTTTATAACATCAAAGGTAAAATTACTTTTGATATTTCACAATATAAAAAAGAAAATTTAATAGATTTTATTAACGAATTAACCTATGAAAAAGCCAAATTCATCAAATTGAGAAATTTGCCAATTGTTAAAAAACTTAAATTTAATGATCAATTTGTAGCAACAATTACAACTATTTCAGCTAAAGAATATGAAGAGTTTAACCCCGTTTTTAAAAACCTTCAAAAAACTTTAAATAAATACGGTTGAAAAGATTTAAAAATTAATTTTGAACAAAAAACCATGGTTTCAGTTGACAACGGTGTTATTGAGGAAGAAAACAAAAGAACTCTAGAATTTATGAAAAAATGACAAGAAAACCAAGAAAAACTTACCAAAGAAAATGAAAGAGTTACAACTCCAAATAATTCGAATAGATGAAGAAGAAAAAGCTATGAAAAAGTTGAGCTCCAAGACATATCTTCGTTAGATGATAAAACTGCTGTTTCATTTACTGGAATGATTTATTCTCAAGACTATGCTTTAAGTAAAAATGGTAAACACATTTACACATTTTTTCTAACCAACTATAAAGATGCTGTAGAAGTAAAAACAATAAGAACAACATTATTAGAACCTGAACAAATAAATGAGCTTGAAGACAACAAATGAGTAACAATTTATGGAACAGTTTCTGAAAGTACTTTTGGTGCTAGAAACAAATTTGTCTTCTTAGATTTCTATGAAAAAGCAAAAAGTTTACTAACAGAAAAGAAAGAAAATAAAGACATAGCTAAAAAAAGAGTTGAGTTACATGTTAGCTCAAAAATGAACACAATGGATGGATTAATTTTTCCTAAAGACATTGTGGCTTTTGCTGAAAAATTGAATCATTCTGCTGTTGCAATTATGGACGTTGATGGTGCTCAAGGTTATCCTGAATTTTATAATGCAGCTAAAAAATCAAAAGTTAAACCTCTTTTTGGAACTGCGTTTTCAGTTATAAACAAAAGTAATAAATCTATTTTAGGAAACATACCTGAAGGCGAAATTAAAGGTCAAGAATATGTTTCATTCGATATTGAAACCACTGGACTTTGTCCTCGTTTTGCCGTAATTATTGAATATGGTTCATTGCCAATTCACTCAAACATGAAGTATGGGAGAAAAACACAATTTTTCATTAAATGTCAAGAACCAATTAAACCTTTTACAGAAAACTTGACAGGTATTACTAATGCAATGATTGAAAAAAGTGGTTTGGAATTAAAAGAAGCTTTGCAAAAAATTTATAATGACTTAAATGGCAAAATTGCTTTAGCACACAATGCTAGATTTGACTTCAACTTCTTAAAAGAAAAATTTAGACAAGCTAAAATAGAATTTCCAAATGTAACAGTAATTGACACACTTATTGTTTCAAGAATTGTTTTCCCTCAAAACAAAAAACATAAACTTGAAGATGTTGCTACTCGTGTTGGTGTTGAATATGATCCTACAGTTGCTCACCGTGGTGATTATGATGCGAAAGTTTTAGCTGAAGTTTGAGTGGCTTTAATGGACGAATTAGGTAAAAAAGGTATTTTAACCTTTAATCAACTTAATTCATATATTAAAGATGATCTTTATAATAAAACCTTTTCATATGAAGTTTCAACAATAGCTAAAAACTATGAAGGTTTAAAAGAACAATATGGTTATTTAACAACAGCTTTAACTAAAAACTTATACAATGGACCTAAAACATTTATGGAAGATTTAGCAGCTCATAGTGAAAATCTTTTATTAGGTTCAGGAACACTAAAAAGTAGATTGCTTGATGTTTATTTTTATTCTTCACATGAAGAATTCATTAAAGAATTAAAACTTTATGACTATATTGAAGTGCCAGCACCTCAAGTGTTTAGCCATTGAATTGATAATGAATTTATTACTCAAGAACAATTAGAAATTTCTTTAAAAGAAATTATTAAAGAAGCAATAAAAGCTAAAAAAATAGTGGTTGCTACAGCTGATGTTAAGTATCTAAATTTATATGACAAAGTAGCTTATGAGGTTTTAGTTTATGCTAAAGGAATTAAAAACTCTCGTCATTACTTATTCAACTATGAACAAGCTAAAAACGGAAATATTCCAATTCCAAATCAAGACTATTTAACCACTGAAGAAATGCTTGAACAATTTGCCTTTTTAAAAGACAAACAATTAATTGAAGACATAGTTATAAACAACACAAATAAAATAGCAGACATGTGCGACAAAATTCAAGTTATCAAAGATAAACTTTATACACCTAAGTTTGAAGACAGCGGTAAAAAACTACATGATTTAGTTTATGAAACAGCATACAAAAAATATGGTGAAAAATTACCAAAAATAGTTGAAGAAAGAATTGAAGCCGAATTAAATCCTATTATTGATTATGGTTTTAGTGTTATTTATTGAATTAGTCATATCTTAATTCAAAAATCAACTGACAATGGTTTTGTTGTTGGTAGTCGGGGATCTGTAGGTAGCTCATTTGTTGCAACCCTTGCAGGTATTACTGAAGTTAATCCCTTACCTCCTCATTATCTTTGCAACAAATGTAAATATTTTGAATTGTCAAAAGATGAAAAAATAACAAGTGGATATGACTTACCTAACAAAAATTGCCCTAAATGTAATATTTTATTAGAAGCTGATGGACAAGGAATTCCTTTTGAAACCTTCTTAGGTTTTAAAGCTGATAAAGTTCCTGATATTGACCTTAACTTCTCAGGCGATATTCAAGGTGATATTCACAATGAAGTTAAACGTATTTTTGATAATACTCACACTTTAAAAGCTGGTACCATATCAACAGTTGCTCTTAAAACTTCATATGGACATGTTAAAGCATTTACAGAAGAAGCAATGCGTAATTATTCAGAACCATTTATTGACTTTTTAGCCCATAAAATAGAAAAATTGAAAAGAACTACAGGCCAACACCCAGGTGGTATTCTTATTATTCCAAAAGAATTTGATGTCTATGATTTTACCCCAATAAACTATCCAGCCAATGAACCTGATTCTTCTTGATTAACAAGTCACTTCGACTTCCATTCAATTCATGACAACATTTTAAAACTTGACTTACTTGGTCATGATAATCCAACAATTATTAGATTGCTTAAAAAATATACCAATATTAATATTGATGACATTCCAAAAAATGATCCTAAAGTTTTAAGTTTATTTGCAAGTACTAAAGCTATGAATATTAAACCTGAACAAATTAATAATGAAACAACAGGGGCATTAGGTTTGCCTGAATTCGGAACAAGTTTTGTTCGTCAAATGTTAAAAGAAGCTAAACCAAAATCATTTGCTAACCTTATTTCTTTAGCTGGACTTTCTCATGGTGAAAATGTTTGGTTGAACAATGCTCAAGAATTAGTAAGCAAAAAGAATTTTGAAATAACTGATGTTATTTGTTGTCGTGACGACATTATGCAATTTCTTTTAAAGAAAAAAGTTGAACCTTTATTTGCTTTCAATATTATGGAAAAAGTTCGTAAAGGTAAAGGTTTAACTGATGATGAAGTTAAGAGATTAAAAGAATATAAAGTGGCTGACTGAATGATTGAAAGTATGAAGAAAATCAAGTACATGTTCCCCAAAGCCCATGCTACCGCTTATGTTTTAATGGCTTGATGAATTGCTTGATTTAAACTTTATTATCCTTTAGCTCACTATGCTTGTTATTTTGCAACCCATGCTAAAGCGGTTGAAATTGAAAGTATGATTGATATTAAAGGTGGACATAAAGTTACAGACAGATTGAATCAATTGAATAACATTCCAAAGAATGAAAGAAAAGTTAAAGATGATGACTTAATTCCAGTATTTGAAATAGCTCAAGAACTTTATGCTCGTGGTTTCTATATTGCTAATATTGACTTGAAAAAATCGGCAGCACATGAATGAATTATTGACAATAAACACGGTTGTTTAATTCCTCCTTTTAAATCAATTGATGGATTAGGTGATGCAGCTGCAGTATCTTTAGTAGAAGCTAGAGATGCTAAAGATTTTACTTCAAAAGAAGATGTTATTCGCCGTACTTCAGTTAATAAAACATTAATTGAAAAAATGGATAAACTTAACATTTTTAAAGGTCTTGACGATACTGACCAAATGAAATTATTTTAAAAATTAAGACCTTTTAAAGGTCTTTTTTTGATCAAAAAATAAGTACTGTTTTGTAACAATACTTATTTAAATTTAGGCTTCTTTAACAACAAAGTTTCCGGCGAAAACGTCTATTTTATAGGCTTTTCCGCTTCTTATGGTGCCATCAATAATCTTGTAAGCTAATAAGCTTTCAATTTTATTTTGGATAAATCTTTTAATAGGCCTTGCCCCAAAGTTTTCATCATATGAAGAAGAACCTACAAAGTCAACAGTTTTGCTTGTGAATGATATTTTTACATCTTTAACATCTTTCATTCTAGTAGCTAATTTTTGAAGTTCAAGTTCAACAATTTCATTAATTACATCTTTAGTTAATGGATTAAATTTGACAATTTCATCAATTCTGTTTACAAATTCAGGACTCAAGAATTTAAGCAATTCAGCTTTAATACTTGGCATTTGATCTAAATGTTTAAGTATTTCTTGTGAGCCTAAATTACTTGTCATAATTATAATTAAATTACGACAGTTAATTACACGGCCTTTTGAGTCTGTAATAGCCCCATTATCCATCATTTGTAAAAGAATGTTAAGAACATCGCGGTCAGCTTTTTCGATTTCATCGAATAAAAGAATTGTGTAAGGATTCTTTCTAATTTTTTCAGTTAATGATCCACCGCTGTCATATCCAACATATCCTGGAGGAGCACCAATGATTTTAGAAACACTATGTTTTTCCATGTATTCAGACATGTCTAAACGAATCATTTGTTTTTCACTATCAAAAAGAGAATATGCAAGAGCTCTTGCTAATTCTGTTTTACCAACACCAGTAGGTCCTGTAAATAAGAAACTTGCTAAAGGCCTATTAGGATCATTAATATTTGCTTTAGCTCTTAATACAGCTTGAGCAACTAATCTAATGGCAGCATCTTGCCCTTTTATTCTTTTCTTTAAATCGTTTTCAAGATTTAACAATTTTTCTTTATCACTTTCAAGTAATTTTGAAATTGGAATTTTAGTTCATTTCGAAACAATGTTAGCAATTTCTTCACTTGTAACACTATCTTTAACAATTGTGTTACCTGATTCTGCTATTTCTTTTTCTGTTTCTTCGATTTGTTTCTTAATGCTTGGAATTTCATCATACAAAATTTTTGAAGCTTTTTCATAATCTGTTTCTTTTTGGTAAATATTTAATCTATGTCTTGCGTCATCTAATTTACTTTTTAAATTAGACAATTCTTCAAGTCTTTGTTTTTCAAAATTTCATTTATCTTCTAATTGTTTTATTGTTTTTTCAATATCTTTTATTTCGGCATCAATTTCTTTAAGTCTAACTGCATGTTTTTTCTTGTCTTCACTAGAAATGGCTATTTTTTCCATTTTCAATCTAGCCATTTTTTGTTTAGCTTTTTCTAATTCTTCAGGTTGAAAGTTTATTTCTGTTTTAATAGTTGCAGCAGCTTCATCAACTAAGTCAATAGCTTTATCTGGTAAAAATCTATCAGAAATATATCTTGAAGAAAGACGAGCAGCAGCTACTAAAGCATCATCTTGAATTTTTACATTGTGGTAATTTTCAAAACGGTCTTTGATACCACGAAGAATTGTAATAGTATCTTCAATTGAAGGCTCTAATACATCCACTCTTTGCATTCTTCTTTCAAGAGCCGCATCTTTTTCTATATATTTTCTATATTCATCAAAAGTTGTAGCACCAATTAAATGCATTTTACCTCTGGCCATTAAAGGTTTAATGATGTTAGCAGCATCCATACCACCTTCAGCATTTCGACCTGTTCCAATAAGCATGTGAATTTCATCAATGAAAACTATAATGTCACCATTAGATTCTTCAATTCTTTTGAGTACGTCTTTTAATCTCTTTTCAAATTGTCCTTGATATGAAGCGCCAGCTATTAAAGCAGCTAAGTCAAGTTCAAAAATATCTTTACCTTTTAAGTTTTCTGGTACTTGACCCTCAACTATTTTTCTAGCCAATCCTTCAACAATAGCTGTTTTACCAACACCTGGTTCGCCGACTAATACTGGGTTGTTTTTTGTTTTCCTACTCAAAATGCGAATCATTCTTCTGATTTCATCATCACGATTAATAACAGGATCTAATTCATTCCGAGCAGCTAAATCAGTTAAGTTTCTTCCATAAATTTTTAATGGATCACTTGATTTTTTACTTTTATTGTCATTAGAACCAAATAAGTCATCTAAATTAAAATCCATAATTTCACCTCCTATAGAAAATCAATTATATCATAATTTTAGCACTTTAAGGGTGAGAGTGCCAAAAATTATTTAAAATATTATTTATTGCTTTTTGAGCTCAAAAATTAAGAATTAATATTAACTATTTAATTATGTGTTAATTGCAAAGCGAGGTAAAAAAATGATTTATGGTTATACAAGAGTTAGCACAAAAAAACAAATTGATGGATATGGTTTTGAAATACAACAAAATGAAATATTGAATAAATATCCAAACGCAATAATAATTAATGAACAATTTACAGGAACAAAATTAAATAGACCACTATTTAATGATCTATTAACTAAATTAGAAAAAGATGATATTCTAGTAGTTTCTAAATTAGATAGATTTGCTAGAAATACAGTTGAAGGTATTAAAGTAGTTGAAGCTTTATTTAAAAAGAAAGTAGCAATACATATTTTAAATATAGGCTTATTAGAGGACACTCCAATGGGTCGTTTTTTCTTAACAACTATGTTAGCAGTTGCTGAATTAGAAAGAAACATGATAGTAGAAAGAACACAAAATGGTAAAGAGATAGCAAGACTAAGAACAGATTATAAAGAAGGTAGACCAAAAGTTTATACTGAAGATCTTTTAAGTGAAGCAATTTTGCTTTTAGATTCCTTTTCCTATCGCCAAGTTTCACTTTTAACTGGTATTAGTAAATCAACATTAGTTAGAGCTAAAAAAGCACTAAAAAATAGCTAGTTTCAAAAGTTACAAGTTATGGGACAGGCTATTTTTAATAGGTGATTTTTACAATTTGAGCATTTTAAAAAAGATAATAAATTTAAATACAATGAAGATTTAAATTTAAAAATTCCTGAAAATTGAGAAGTTAAAAAAATTGCTGAAATTTGTAAAATTTTTCTTGGAGGAACCCCTAGCACAAAAAATAGAGAATATTGAAATGGCGAGATAAATTGACTAAATTCTGGTGAGGTAGCCAATTTTCCAATAATAGATAGCGAAAAAACAATAAATGAAAAAGGTTTAAAAAATTCAAACACTAAATTATTGAAAAAAGGTACTGTAGTTATTTCTATAACAGGAAATATAAGGGTCAGTTATCTTGCAATTGATTCATGTATAAATCAATCTATAGTAGGAATAGAAGAAAATGAGTTATTGAAAATAGGATATTTATATCCTTTTTTAAAGAATAAAATTGAGTTTTTAATAAGATCTTCTACTGGGAACTGTCAAAAACATATAAATAAAAATTTTATAGAAAATTTAAAAATTGTTTTACCACCAAAAAATGTTTTGGACATATTTAATAATTTAACTCAAAATATATATGCTAAAATAAGTCAAATCAGTTTAATGACTAAGAAATTAATAAAATTCAAAAATAAGCTATTACCTTTATTGATTAATCAACAAATTATTTAAAAAAGTCCTATCAATAGATAGGGCTAATTTATTTCTATTTGGCCATTTATTAATAAAGGTATCAACTTTTCTTTAAGCTTTATCAAACTTAAAGTATTTTTTTCTAATTTCATGCATTTAGTTATTAATTCGTTTAATAAATTGTTTTTTTCGTTTGTTAATATTGGAACATTCACAATATTGAGATGATTTTTGTTAATAATTTTTTGTATAGCTCCAATTGATTGTCTTTTTAAAAATGAACTATATTTATTTAGAATAAAATAAATTAGTCCAAATTTTTTTTCATTATAAAATCCAACCATAGCTCTCCCAATTGCAATATTTTTATCACTTATTCCAATTTTACCAACAGTTCCCGCAATAGACATACAAATTGTCCTATTATCTATTATTTTAATTGGTTTATCAGTAAAAGCTTTAGGAGAAATTAATATTTTATTATTCTCTAAATCACCTGCTCCTGATGCATAAAGCATTAAATTATTTGATACGTTTTCGCTTAAAATGTTGTTTCCTTTTGGACACTGACCCCAAATTATTTTGCACAAACTTTCTATATTTTCATAATCTTTCGCATTATTCAAAAACATATCAAAAATAGCCTGTCCCATAACTTGTAATCTTTTGACTATATGCAAATTCTTTTCAATCTGCTTGTCTAAATTTTCTAATATTTTAGAAATCTTTTTTTGAGTTTTTAGTGGAGGCAAATCAACAATTTTTTCTTCCATTGTTTCTTTTGTAATATGAATCATTATTGATCCATTAGTTGATGAAAGCCAATAAGGTGTCATATACATTAAAAGATAATAAAGAAACATTTTATCGACGCGCTTTTCATCTCATTTTATTTTTCATATATGATAATGAAATATTGTTTTTTCAGATTTCCAAATTTCAGGGCCGAAATTACAAGCTCATTTATATAACAAATCGCCATTTTCACAATATTTATCTTCATTCAATTCCATATCTGAATAAAATCATTCATTTTTACCAGAAAAATTTCCTACTCGCACTATTCTATATTTTCCCTTGTCTTGTAATTCAGGTTGAGAATAGGCTCTTCCATTAATGAAAGTTACAATATCTTTCAATTTAACAAGCATAATTTGCCCCTCACTTTTTTGAGAAAATTAATGCTTGCTCAAAAGACTGTAACTTTTGAACCATAGAATTATTTCGCTCAATTTGATTATCTATGTTATCTAATATTTTTGATATTTTTTGGCACTTATTTAAACTAGGAATATTTATTTTTATGTTTCCTAAAATTCCTTGAGTTAATAAAGGCTGTGAAGAACCTATATGAAATTTATTTAAGTTTAAATGTAAAAGAAAATAAAAATAAAATCTTGCCATATTTTTTTCTTTAGGTTTAAATATTAAAGCATTATCTGTAACAAAACTTTTTTTGTTTGTGATATTTACACAACCACAATTCGCTCCAACTCTACCAATAATTATTGCATCTTCATCAACATTTCATTTATTCACATATGTAATAATGCCATTGCCTCCAAAAGCAGGAAAATTTCCAATTTTTTTGATTAATGTGCTTCCAGTTTCAAATATACCTAATTCTTTTAATTTATATTCCATATTTATCCTTATTAATTTATTTTAGCTTCAACTCCTTCAATGTTTTAGACATCTCTTTTTGGAATTCTTCTGTATCTTTTGTAAATTGTTCTAATTCTTTATTAAAGTCATCAATCATTTTGTCAAATTCTTCTTGAGTAATATCAACATATTCAATTTTTACTTCAAAATATTGACCAGCTGATAATGAATAGTTTCTTTCTTTTATTTGATCATAGGTAACTGAAATTGTAAAATCATCTTCAACTTTATGATTAACAAAATCATTAACTATTTTGTTTAATTCATCATGAGTTAAAACTGTTTTTTGAAGATCTTTAACTTTTTCTTTATGACCCATTTTTGAAGCGTCCATTAATAACACTTCACCATTAGTATTTGTTTTATCTATAAATAAAACGGATACGTTAGTTCCAGTATTAGCAAAAATATTTGATGGCATTGAAATCACACCCTTTAGTCATTTTCTATCAATAATATATTGTCTAATAGTTTTTTCAATTCCACTTTGTGCTGTAATAAACCCTGTAGGTACAACTATAGCGGCTTTACCACCTTCTTTTAATGAATAAAGAATATGTTGAATAAATAAAAGATAAATAGCCATTGAATCTTTTTTAGTTTTGGGAACTTTAGGAATACCAGCAAAAAATCTTTCTGTATTTTGTCACATTAATTCAATGTTATCTCTAGTAGAAGAGAAGTCAGTTTTGAATGGTGGATTAGATGTAATAAAATCAAATTGTTTAAGACCTGTTGTTGCGTCTCCTGCTTTTTTATAGTGTGTTGGGGTAAGTAAGGTATCGCCTTCAATTATGTTATCTAGTGATTCTTTTAAACCATTAAGTAACATATTTATTCTTAAAAATCTTGATGATTTTTGTGAAATATCTTGAGTGTAAACACGAGCCTTATTTCCAAATTTACCTTCTCCAAGTTCATTTGCTAAATGCAACACAAGCGATCCAGAACCTGCACTTGGGTCATATATTTCATAAATTTTATCTTCAATTGGAGACATTTTAACTAATATTTCACCAATAGCTTTTGATAATGTTTGAGGTGTAAAGTATTCAGCATATTTACCACTTGCAATGTTGTAATCTGAAATTAGGTATTCAAAAATAGTTGAAAAGAAATCATAGTTATTATTAAAAGCTTCACCAAAATCAAATCTATCTCTTGTAATGTAAGAAAAAACATTTTTTGTGAAGTTATTTCTTTTTGAAGGTTCAACAAAAGTACTAAGACGAACAAAAAGTGGCTTTTTAGTTCCTTCTGCAGTATCAACATTAAAAGCTTCATTTTGTTTATAATCTGAAATTCTAATTAATGTATCGTCAAATTTTTGATAAAAATCATCTTCATTAATGTATCCAACTAAATAATCAATTGTATCTTCATATTTGAATGCAACATCTTGTGGATAAGCATCATAAAATCCATCCATATAGTTTGAATTTTTAATTATTTCTTCAAAAGGAACATTTAATTCTTTTGCAAATTTTTTTAAATTTGAAATGAATTTATCATTTAGAAATTTATATAAAAAGACAGAAGTAACAACTATTTCTTCGCCAGCTGTATTTGAAAGACCATTTGTTGAACACAATCCCTTCAAATCATCGATTATTGATTTTATATTATTTTCAAGTTTTGTAAAATTAGTCATTTTTCTCCTTAAAGTGTTTCCTTGTAATTAAGCAGATTAAAGTATAATTCGTTCAAAATATAATCGTAATTATCTTTAATTTGTTTATATAAATTCTTCTTAATTAATCTAGTTGTTATTTTAGATTTTGTTGAGTCAATAAATCCATTTTTGCCTTGTTTAATGAGGATTTCGTCAGAAATGTTGCCTTTTAATTCATCATAAACCATTAATAAAAAGTTCTTTAAAATTGAACGATCTAATTTAGTTTCTGCTAAAACAATATTCATTGTTGTGACAAAAGCCAAACTATTTCCAAAGTCTTTAGATATTTCTTTATTTTCTTCATTTATTTTTTTAGTTGAATTGTAAATTAAATCTAATTCATCTTCTACTTCTTCGTAAGTCAATTCCTCAACAGCTAATTTATCAAGTAATTTCTTAATCAATGCATTAAGATTCATAAATTTAACATCTCTTGTATTTTTGTTTTCTTTAATTTCTTTTTGCATTTTCATAAACTTTTGTTTAATAAGTTCCATTTTATTTTCTTTATCAAATTTACCTAAATCTAAAACAGTAATATTTTTTAAAAGAAAATCATAAATGATTTGAACAACTTCTTTGTTATCTATTAATTGAATTGTATCAATAGGATCTGAACGCAAGTTTAAAAAATTAATTTTCTTTTGTACTTCTTTAATAAACACATTGATTTCATAATCGTTAATGAGTTTTGAATATTCATCAGCTTGTGATAATTTGAATTCAATTGAACAGTTTTTAATATTAACAAGAATCTTTTTGATCTTTTGTAAAGTATCTTTGTCAAAATTGTTAATTTTGTCAACAAAGGTTTCTAAATTGTAATTATTAACAAATTTTTCAAGATCACTTTTTAAAACATCATATCTTTTTTTGATATCTGTTTTATCAATAATTAAACCAGAAAGGGAAGTGTCTTCTTCTTCAAAATCGTATTCTTCTTCTATTTCTTTACGATAAGCATCAATAGTATTGTTGTATTCTTTTTCAATATCAATAAAATCAACTATATATCCATATTGATATTCTTGGCCATTAGGACTACGATAGGGTCTATTAACACGTGAAATAGTTTGCAAAAGATTTTGAGCTTTAGGTCCTCTTAATAAATACATCTTCTTAAGTCTTTTAACATCATATCCAGTTGTAAGCATATAGTGAACAACTAAAATATCTGGATAATTACTTTCTTTAAAGGTTTTTTGATTATTTTTATTTATGTTGTCTTGCAATGGATTATCAGTGTCTGATAAAACAAGGCCAGTAGTTAATTTAGAATTCTTTTTAAATCATTCATGAATTTTTTTCGCTTGGTCATTTGAACGACAGACAATCATTCCACCAATTGTTAAATCATTTCTGCAAATAAATCTAAAATCAATAAAGTCTTTTTCTATATATTGGCTAACGCTTTTAACATAATCATCTGATTCATAAATAATTCTTGATTCTAATTTTTCAATTTCAATATTTAAGTTTCTAATAATTGTAGCTCTAGCTTCAGTTTTAATTTGTTCTTTTTTAATTCTCAAAGTATAACCATCAGCTATTGATTTATCATAAAAGTATTTATGAATGTAGTCTCCAAATTTTAAATTTGATCTTTCTCTTTTAGTTAATAAAGGTGTACCAGTCATTGCAATGTAAATTCCATCTCTATCACAAGTCATAAGGTTTTTGTAAAATTCACCATGCATTGTATAACTTCTGTGAGCTTCATCAATAAAGAAAATTCTTTGAGTTCTTATGTTGTAATCATTTTTAGCTTCTGGCATTTTTGAACTATCCATAAATTTATGAATGTTTACAACACAAATTGTGCCAATACCATTATTATTTCTATCGATTGTCTCATTTAATTCTTTAGCGAATGCTTCTTTTGAATCAACATTAACAACATTGAAACCTCTATTGGCAAATTCTGCAGAAGCTTGTCTCATTAAGTCTAATCTATCAACTATAAAGAAGAATCTTGCATTTATTTTCTTTTTACTTCAATAATCTTTAATTACTTTTGTAGCAAAAGCACTTAGTGCTGTTTTGCCTGAACCTTGAGTATGTCAAATAATTCCATTTTTATTAGTTCGTTTGAATCTTTTGATAATTGCTCTTGTAGCGAAAAATTGAGGATATCTCATAATGTGTTTTTGATTAATTGGCAATTGTTCAACTTTATTTAAAGTTTCATTTTTAATTTCTTTTATTTGGCTAAGATACATCATTCCATATTTAATGAAGTATAGAAATCTTTCTTTGTCAAAAATTGAAGTTATAAAACGGTTGCAAGGAGTTGTATCTGATAAATTTGTTTGGAATCAATCACTATCAACATGTGAAGTTTTAATATTATTATCAATTAAAATTTCTTTTATTTTGTCATTGTCTATTTCCTCAAAAGAGTAGTTAGCATGGTAGTTAGGTTCATCTTCTCTAAAAGCTGAAAAAGTAGTATTGTCTTTTCCATTTGGTGTTGTATAGAAAGAGCCTTGTTTTAGTAGTGAAGCCTCAGCATCATTAAATTCTTCATAATCCATATTGTTTGTAAATGAAACAATTTGCAACATATTAAAAAATTTTCTGTATTCATCAACTTTTAATCTATCTTCTCACATTCTAATAAATTCTGCTTGAATACCATTTTTGTTGTTTTTAACTTTTACTTCTAAAAATGCTAATGGCATACCATTAACTAAAATTGTAATATCTGGTCTAAATGAATCATAATTAGAATTAGCGTTACTTCTAAAAGTTAATTCATTAACAATTGCAAAATCATTGTTCTCAATATTTTCAAAATCTATCAGTTTTATAGGGTAATCGGTCGAAATTAATCGATTATAAAATTCTTTTCCTAAATCCTTATTTTTGATTTTTCTGCTTATATCAGAGATCAATTCATTTATTTCTTCTTTAGCAAATTTTTTCTTATTTATTTTTTCAATTGCTCTATGAAATCTTTCTTCAAAAATGTTATTGTCTCTATTAATTTCTGGGCTTCTAACAGTTTGATAATTGTAACCAAGTTTTAAAAAGTGAATCGTTGCTGGTAATTTTACTCTTGTATCTTCATTAAATTCCATTATCTAACCTCCAACAAAACACCATTTTTTCTACAATATTCATCAAATCTCAATTTAGCAACTTTTGTTGGCTTAACTTTTCCATTTTCCCATCTATTTATGGTGTTAAAAGAAACACCAAGTTGTTTTGCTAATTCTTCTTGAGTTAAGTTTAAAAGAGCTCTAGCACGTAAAATTTTGTCTTTAAATTCCATTATCTTTTGCCTCCATTCGCTAACAACTTATAACAAATTATAACATTTTATTTTTTTATAAATATTAAATTAATATTTAACTTCCTTTATATAGTAAAATAGAAAACATGAGATTAAGAAATGACAAAGATGCACCAAAAAAGCTTGAAGAATCTAATTTATTGATTACTAAAAGTCAATATCCTATTAGCTTAAAAAATACTGATGTAATTGAAATTGGCATGGGTAAAGGCGAAATGATAACTCAATTAGCTGCTCTAAATCCAAATATAAATTTTTATGGTTTAGAAAAGTTTGCTACTGTAGCTGCTAAAAGCGTAAGAAGAGCTAAAGAATATAAGCTTAATAACTTTAAAATTATTTTAGAAGATGCTAAAGAATTAAAAAGCATTTTTAAAGGAACTTGTGGCACAATTTGATTAACTTTTAGTGATCCTTGACCTAAGGCTAGACATTTTAAAAGAAGATTAACTTACAAAGATTTTTTGGAAAAATATAAACTTATTATGGATAAAAATACTATTCTAAAATTCAAATCAGACAATGATAAACTTTATGGATTTTCGCTAGAAAGCTTTAAAGAAAACGATTGAAACATAATAGCATATGGTACAGATTTTCATAAAAGCAAATATGCTAAAGATAATGTTATGACAGGCTATGAAGCAAAATGAAGTCAAGCTGGCAAAAACATAAATTATATTTTTGCTAAAATTAAATAGAGGTATATGGTGCAAAATTTTAATGAAAAAATAGACAAATTTAAAAATTGAATTAAAGAATCTAAACATATTGTTTTCTTTAGTGGAGCTGGTGTTTCAACAGCTTCAGGAATTCCTGATTTTAGATCAGCTGATGGTTTATATTCTAAAAAATTTAAAAACATGAATCCTGAAAGTATTCTTTCAAGAAGTTTTTGAAGAAAAAATAAAAAAGATTTTTATGAATACTATTTTTCAAATATAGCTTTTGATAACATTAAACCAAATATTATTCATGAAACTGTGGCAAGCTGATGCAATAAAAATAAATGCCATGTTATTACACAAAATATTGATAACTTGGATATTAAAGCTGGTAATAAATATGTAATTGAATTACATGGAAATATTAATCGTAATTATTGTTTGCTCTGTGGTAAGTTTTATGATTTAGCTCAATTAATTCATCAAAAAGATAAAGATGGAATTCCTACTTGTAAATGTGGGGGAGTAATAAATCCAGATGTAGTTTTATATGAAGATCCACTAATGGAAGATTCAACTAATGATGCAGCTGAAGCCATTTCAAATAGTGATTTATTAATTATTGCAGGTACTTCATTAAGTGTTTATCCTGCAGCTTCATATATTCATTTTTATCAGGGTAAAAGAATAGTAATTCTAAATAAAGATACTTCACGTTATGAAAATAGTAACAATGAAAATATCTTGTTGTTTAATGAAAATATGAAGGATGTTTTTGAAAATTTAGCTAATTCATAGTTAAATGAAACAATTTTGATAAAATAAAATTATCATGAATAAAGATGACAAAAAAGAAAACAGCTTAGAGATTACAACTCTATACGATGCTGAAAAATTTAGAATTATTAAGAATGAAGATTTAAACAATTTAACTAATGAACAAAGAGAAAAATTAGTTAAAGATGCAATTGATAATGCTAAAAAAAATGAAGCCAATATAATTGCAAATAGGAAGAAAAGATATTTGTCTTCTTTAGCTGTAATTTTAGTAGCTGGAATAATCATTGCAATTACAGTAATTTTGATACTTGCACTAATTATTTACTTACATAAAAAATAATTATATAAAAAAGTTGCTTTTTGACCAAAGCAGCTTTTTTATACCATTTCTAGGAAAGGGATTATTTTTTTAACATACAATATTCCTGAGTAAATACTGAAGAATAATGCAACAATTGTAAGAGTGTTAATTGCATAAATACAAGCATCACTTTGAACTAAAGTTAGGCCATATTTTTCAACCAATTCAATAGGATATTCAAAGCTTATAGCAATAATTAAAATAGCAATAATTGCAAGCGTTTGAAACAAGGTTTTCATCTTTCCTCAAATTGAAGCCTCAACCCCAATTTTCTTTTCAGCCATTATTACACGAGAACCATCCACTATCAAATCACGAACTACAAAAACTATAACAATTCATAAAGGAATGAATTTAAAAGCAGCTAAAAAAATTAATGTACTTGTGACAATAATTTTGTCAGCTAAAGGATCTCACAGCTTTCCAAAGCTTGTAATCATATTGTATTTTCTAGCAATTTTGCCATCAAAATAGTCAGTAATCATTGCTCCTAAAAAGATAAGTAAAATTCCAGATAATATTGAACGGCCAGCTATTTTATAGTAATTATCCATGTAATTTTGGATCATCTTATAAGTAATAAAAAGAGCTAAAAGAGGTAAGAATAAAATCAATCTAAGTATTGTTAGTTTATTTGGTAGATTCATCTTCTTTGTCATCTTTTAAAAACTCCGCATCATAATAATCATTAACTCTTTTTTCCACTCTTTGATATTCATCTTCATAAAGAGAAATGTTATATGAATTAAATTTTTCTTCTATTCATTGAAGTTTATCAGCACATCTTTTACTTCACGAATTGCTTCTTGTATCTCTTAAAAAACCAAAAGCAAAAATTTCATCCCTAGCATCAGCATTATTCAAAATACATTCTTTAAATAAAGCATCTTCTTGATATTTTTCTAAGAAACCACGAGCTGTATTAATTATGTTTGACATTTTGTATTGACCAATTGATGGTTCAAATTCTTTTAAATATTTTTCATTTTTGTCAACTAATATTTTTAAAGCAAAAATAAGTAATCTAGATTGTTGTTCAGTATCTTTTTTGAATTGTAATTCTTCTTTCTTTCTTTTCTTTTTCTTGATCTTTTCTTTAACAGCACTTCATACAAAGAAGCTGGCTAAAACAGCAACTAAGATACCAAGTATTATCCACATTGTTACGCCACCCTTCATATAACCTCCTATAAAATAAATGTATAAGTAAAATAAGTATATTAATTATAAACAAAATATTGATTTTAGTTTTATAATTTTTTCCATTATGGAAAGAGAAACTAACTTAATAAAAAATAAGAAAATAAATGACATTTTTGAAGCAATTGGAATAATTGTTACAGTTGATAACGATTTAAAAACAAACAACAAAAAACATAGAATTACATTATGAATTTTTAGAACACTTTATTTAGTTTTCATTGCACTTTTTGTTCTATTTGGATTTGTTAGCTTTATTTTATTAAAGTATTCAAATCATAAAAATACTTTTGATAAAGCAATTAAATTTATTGAAATTTCTTCATTTTTTGTTTTTGTTGTTGACTGATTAATACATTGAATCACTTATCCAGCAAGAGACAAGAAAAGTAAAAATATAGGAGTTTCATATTTAAAATATTTGATTACAAGCGGCAATATTATTTTGATTCTTACATTTTTGCCTTCACTTTATGTGATTAATAATTTCTTGCCTGAAGCTAATCAAAGTAATGCATTAAAAGCTTTTAACGGCTTTAAGTTTTTAAGAATTTTAAGATTAATTATGCTTTTAAATGTTTTTGTAGTTTTTAAAAATATTTCAGAATCTGTATCAAATGAAAAAGTTTTGTTTTTCAATATCTTCTTATTTATAGGTATTTTGATCGTTTTATTTGCTCTTACAGTTTGATATACAGAAACAGAATATGTAAATAATTTAGTTAGAGAATTACCTGAAGTTAAAGCTAATCCAACTAAATTTGAAAGTGAAAAAATTAAGTTTTATACAGAACACACCAACATTGTAAAAAACTTTGGAGATGCCTTATACTTTAGTGCCATAACCTTAACAACTATTGGTTATGGAGACTATATTCCTTATAGTGGTTTTACAAAAATGATTGTGCCCTTAATTTCTATTGTTGGTATTGCAATTATTGCTATACCTGGTGGTATGGTAGCAGCAAGTGTTTTAACTAGTTTTCAAAAGAAAAAAGATAATAAAGAAAAAGCTAATGAGCTTGCTGAAATAATTGATGCAGAAATTGAAAGAAGAGTTAAAGAAAGAATTAAAGAAGAAGAAAAAGAAATGAAACACGATTTATTACATCGTTTTGATGAAACAATTCAAAAAGAAAAAGAATTAAAACACACAAACGAACACGCATCTCATGATCATAAAGTTCATAAATAATTTACTCATTAATTTGAGTTTTTATTTATAATTTTTTTAACGGTAGTTGCTCGAGAGGCTGAAGAGGTTGGTCTCGAAAACCAATAATGGTGAAAGTCATTCAAGGGTTCAAATCCCTTACTACCGGCCAGATGAAAAAAACTTGCCAATCGCAAGTTTTTTTGTTTAGTTTTTGATTAAATAAGGTGGAGTTTTATCCTTAAGAGGTCTCTCAAAATCAGCATCAGTTAATGTTTTTAATTTTGCTTCAATATCACTGAAATCATAACCAACAAAACCGCCTAATAAGAATTTTCTTTCACTTATGTTTTTAATCATATCTTCATTTTTATGATTAATGAAATCTATTCTAATTGGTAACTTACCGACGCCATATTTTAAGGCGTCATAATCATTATTTGGATCAATAGTAACTTTAACTTCTTTTATTAATTCTTCATAACCTGGTTTTTTGTTGTTTATAAAGAATTTAAGGACATATTGGGAAATTAATGTACTTATTTTTTTTTCAAATTCTGCTTTATTTTGAATTATTTCTAGAACACTATTTTTGTTCATTTTTTTATTATTTCAAATGTTAAATGAATCTCTGTTTTGATAAATAATAAAGTTAACAATATCTATTAAATTAGTGTTATAAAATTCTTCTGCATTAATGCCTTCAGGTAATTTTAATTCATTATTTGTTCCAATTCCTTTATTTATATAATAGCTGAAGAATTCATCATTTTTTAAAGGATCACAATCTAATTTATTTTGAATATAAAAGCCATCAAATATATGTTTATGTGCATATGTAAAGTCAATAGTAAACCAATTAGCTTCTTGTACTCTATTAGTTTTTTTGTTTTTAATTTCAACTATAGCCTTGCCTATTGAATGTGAATTATTTAATATTTTGTCAAATTTTATTTCTTTTATTTTTGCTTCATAATTTTCATCAGGAACATTAATTTTGACCTTTTCTTTATAAGCGTTATAGAATTGCATAAAAGAATTAAGATTAAAACCTAAATAAGGTTTTGAAGGGTGCATTAATGAATCAAAATCATTAAAATTGAACATATTTTTTTCAAATGAAAGTGTTGGTTCCTTAAACATTTCATTAAATAAAGATTTGCTTTTTTCATTTGTTTCTAATGAAGAATCGATTTCAAAATTTCCATATTTTTGAAATGCATGATATCTAGGATTAGAATCTTGAAAATCCATCAAATAAAAGACTTTATTTTTTTGATCATTATTTAATATACTTTGATTATTTTTATCTAATAAATCTACTTTTATTTTTAAAATATTCTTGCCTTTGTACTCTTCTTTTTCTTTACTTGTAATAATTAATTTTTCAAATTCTTTATTTTCTTTATTAACTATTTTATAGGCTTTTAAGTAAAAAGTTGCTTGTTTTTCGAAGTATTCTTTGAATTGATTTAAAATAGCATTTTCATCAAAAATATTTTCAATTCTTAATAAGTTTGAACTTAGTTGATTTTTTCAAATTGAAAATCTATTACTTCCTGAAATTGCAACTTGTAAAATCATTTGTAAATCAGGTAAATTTTTAGAAGCATTTGCTAAATTGTTGTATTTTGATTTAAACAATCTTTTAACATAATTAACATAGCCTAAATTTTTAAAATTTGAATTATCATTTTCAAAAAACACATTTACGTCAGTACTATTGTTGCCTTTTCTAATAAGGCTATTATTTTTATTTGTAACAATTTCTCAATTTTTTGATAATATATCCCAATTATCATTAACTGATTTTAAAAAACTTTTTAATGAATATTTTTTATTATTTATTGACAAAAATAGATTATCATTTTCTTTTACAACTTGTTTTAGTTCAAAATTATTTTTTAATCTTAATTTATTAGTTTGCTCGATAAAATACTTTATTTGATTTAAATTTAATTTAAGTAAATCTTTGTCCTTTAAGCCAATTATTGAACTATTTTCATATTTTGATTCAATTAATTTGTCTTCAAAAGGAACTTCTTGTTTTTGAACACAAGAAGCAGATATTATTGGAGCAAAAGATAAAGCACTACCTAAAATAAATCTATTCAAAAAATATTTTTTCATCTTCTTCTTTCTGCTAATGTTTGTCATACTCATAAAAACCATTTAATTTTAGATTAAATGCATATAATTTCAATAATGTTCTTTCATTTTCTCTTATTTTAGTTAAATCAACATGGAAGTGCTCACTACCATAACTTCTTGTATTTGTTATATTAATAGGCGAGCTATTAGTATTAATCTTTTGATAATAATTTTCATCAGAAACATCGACAATTTTAAAACCAACCCTAGCTCTAGCATTTCTTAATGTTCCATTGTCATAAACTTCCAGAACATCGAATCTATACTTAGGATTTTTAGATTTAATTTTTATATATCCTCTTATTCATAAATATTCAGCAGTAAATTCTGATGGTTCAGGATTTATTGTTGCAGAAGGATTATCAATTAATTGTCTTGTAGAAGGAGGTAGTACATCTTTTGCATATTTCTTCTTGAATTCTGGATTATTTGTTCATTCATCAGCTATAGCTTTGCATTCTTTTTTTAAGTCGTCAGTAGAAATTGAAAAATTATAATCTTTTCCTTCTTTTAAATTGTCAACAAAGTCTTGTAAAGCTATTCATTTTTCTTGATTTCCTAAACAACTTACTGAAATCATTGGTGTAGATAAAACACCAATAACTGATGGAACTAAAAGTCACTTGAATTTTTTCATTTAATTCATCTCTTCTCTACATCATTTTTAATTTAATGATTACTTTAAAATTATACAAATTAATAGTAAAAATTTCATTAATTATGATTTAAAAAAATTATTGATTTACCAATAATTCTATTTTAAAGTATTTTTAGAAAAAAATGTTATTTAAATTTATCTTTTGCTAAACAATATAAACCCATTACAGCGCTGAAATCTGTATAAGGATCAAAAGCAAATTTTACATTTTGATATTGAGATTTAATTGTATATGTTTTAGCTTTTTCAATAGCTTTTTCTATAAACCAATTATTATGTTCACTAACACTACCGCCAAAAACAACAAGACTTGGATTTAAAAAAGCTAAAGAAGTTGAGATAGTTCTAGCTAAAGAATCTATACCTTCATTTATTATTTTTTTTGCATTTTCATTATTTGGATATAAAGCAAAAATTTTTTTAGCGTTTAATTTTTCTCCTGTTAATTTGTTATATCTTAATTCAATGCCTGTTCCTGAAGCATACAATTCCACTGAATAAGGAAGTAAATGAAATGAGTCATCTTCATCAGTACCTAAAGGTGCTCTCGCGATTTCTTGAGCTAAGTGATTTTTACCAACATATATTTGATCATTAATAATAAGACCAGCACCTAAACCTGTTGATACTGTGAAAAATTGAGTTACATCACTTTTCTTTTGTTTGTAATATTTGTGATTTGCCAAAGCCATAACATTAGCATCGTTTTCAGTAATTATTTCTTTTAGATTAGTATGCTTAAATAAATATTTTTTAATGTTTTTGTTAACTCAACCAGGTAAATTAGGTGTATTTAAAACTATTCCTTTGTCATAGTCGGCTGGTCCTGGAATACATAAAGCCAAGCATTCTATTTTATGCAAATTGCATAAATGAGCAATATGATTTAAAGTTTCACGATAATTATTTTTATTAGTTTCAAATTTAACTTTAGATCAAAATTTATTTTTTTTAAATAAAGCGAATCTTGTATTTGTTCCGCCAATATCTGCTGTTGCTATTTTCATAATTGTTTATCTCCTTCTAAAATTATAATTTAATTTTTACGTAATTTTTTTATAACTTGAACATACAAGGCTATAAAAATAAAAATTTTAAATATAATTATTAAGGAGTAAAAATGAATAAACAAAAGAGATGATGAATGCTATTAAGCTATGTAATAATTGGCTTAGTTTTATTAATAATTAATGTTGTATTAGTTTGTTGTTGACAAAAAGGTAGTGGAGTTTTTATATCTTTAATTGGTCAAACAATATTAGTAATGTTGCCTTATATTATTGTTGGCTACAGTCATGAAAAATTAAAATATTTTTCGCATGTCAATATATCGTTAGGAGTAATCTTAATTATCTTAACTATTTCGCAAGTAGCAGTTTCAACTAAAGCAAATTTCCTTATTTATTTCTTAATAAGCTTTGAAATAATTAACGTTATTTGCATAACTATTAGTTTGATTTTTGGCATTATTTTCTTTTTTGATTATCTACAAAGTTTCAAAGAATCTTTATTAATATTTAAACAATATTTAAAAGAACTTTAAATAGATTACTAACTAAAATTTTAAAAAACATCACACTGATTTTTTACACTAAGTGTGATTTATTATGCTGTTTTATAGTATTCCAAGTAAAATCTACTTAATTATTTTTTTGATTCAGAATTCTTTTTTCTTGCTGTAAGGATGCTCATTTAATTTAAAATTAAAACCTTTTCTTTGGTAAATAGCTTTTGGATTTGACATAGCTTTAAAACCTTCAAAACCATGACTTCTGCCATAGCCAGAATCACCAACACCACCAAAAGGTAAATTACTATTTGCTATATGCATAATGGTATCATTAATTACTAAAGCTCCACTAGTAACTTGTTCTTTAAATAGCTTTATTATTTTCTTATCTTTAGTAAATAAATAAGCAGCTAAAGGTTTAGGAAGTGATTTAAATTTGACTAATAAATCATCTAAATTATCATAAGGAATAATAGGCAACAACGGCCCGAAAATTTCTTCTTGCATTATAAGATCATTTCAGCTTGTTTCAAAAATATAAGGCATTATTTGTAATGTTTTTTCATTAATTTTATGATCTTTAGGTAAATATTTTTTTAATCTTTCAAAATGTTTTTGATTAATTATCTTTGGATAATTACCTTTAATATATTCTTCATTTTGCAAAACGTTTTCAAGATAAACATAACAAGCAGCTTTAAATGCGTTAATTCTATTTTTATCAATTAAAACATAATCAGGAGCTATACAAGTTTGACCTGCATTAAGCAATTTACCAGCCATAATTTTTTGAGCAGAATAAATTATGTCTTGACTTTTGTCAACAACGCAAGGGCACTTCCCACCTAATTCTAAAGTAATTGGTGTCAAAGTTTCAGATGCTTTTTTAGCAATAATTTTTCCAACTGTTGTGCTACCTGTGAAATGAATTAAATCAAATTTGTAGTCCAACAATTCTTGAGCAACTTCTAAAGAACTGTCTGCTACAAAAGCATATTGAGGATCAAAATTATTGTTGATTAATTCTTGTAATAATTGTGAAGTGTGGACTGAAAATTCACTTGGTTTAATGATTACTCTATTGCCTGCTGCAAGAGCTGAAACTAATGGCATTAAACTCAAATAAAAAGGATAATTTCAAGGACTAATTATTAATACTTGACCTAAAGGTTCATATTGAATTTCACTTTTTCCTAAAGCTAAATGAGAAGGCGTTGAAACATGAAAAGGCTTAGTTCATTTATTTAAATTTTTAATATTAAATTTGATTTCTTTTAAGACTAAACCTATCTCACTAAAATATGATTCTAGTGATGATTTATTAAGGTCCAAAGATAAAGCTTCTTTAATTTTACTTTCATTTTGCACAATTATTGAGTAAAGTTTTTCGATATTTTCTTTTCTCAATTTTTTGTCAATAAAAGCATTTTTTAATATTTTTTCTCTTTGCAATTGATAAACATTTTTTGCATTCATAATATCTCCTGTTTGTCTAAGAATATTATAAATAAATAATTTTGTTATAAAAATTATATTAAATTTGTAAGTTATTTTAAATTTTGATCTTATTTAAATTAAATTTTTTGTACACAAAAAAAGCACTAAAAAAATTTAAAAATAAAAGTATAATTCAAAATATGAATAATCAAAATCAACAAGCAACTCAATATACAAAAAAACCAACATTTTGAAATGTTTTTTTGTTTATTTTATTTATTACTTTCATAGGTTTTGGGGGTGGCAATGCCTTGATGCCTGTTATTAAAAAGTATGCTGTTGATAAATATAAATGATTAAATGAAAAAGAATTTGATGAAAATGTAATTATTACAAATATGCTTCCAGGTCCTAGCGTCATTGAAGCAATTAGTTATATTACTATTAAACTTTTAGGTTTCAAAAAGGGCTTGTTTTTGGCAACTTTTGCAACCTTACCTCATGTTTTAGTTTTCTTTTTAATCTTTTATTTTGTGCAACATATTCCTCAAAAATATTTGTTTGTTATTGAACTTGGTGTTATTGTTGCAATTATTGGATCATTGATTGGTTTTGCTTGGGAATACTTCAAAAAATCAAGAGGCAAAACTAAAATTAGTTTGTGATTAATTTTATTTTTAATAACCTTTGCCTTTAGTTTCTTTATACCAACTCCATGAAATATGCCTGTAATAATTATGGTTCTAATTATTTGTATTTTTGCAACTGTTGAAGCAATCAAACACAAAAAAGAAATCAAAAAACTTAAAGAAAATATTGAAAATAAAGTTACTAATTTAGACAATAACATAGAAGAAAAAATAGAACAAAACTTAGAATTAGAGACCAAAGAAAATGATTTGAATAGTGACAAGAAAGGAGGTCAATAATGGCTTGACTTGCTTTACTTGTTGCCCTACCTTTTTTGGTATTAATTAGCTTATCTGTTTTTGGCGGTGGGCAAATATTTATGCCTATTTTTAGTTGATTCTGAGGATTATTTAGAGAATGATTTGGAAGTAATATTACTGAAAGTGATATTAGCCAAGTATTTGCTGTTGGTAATGCAACACCTGGAATTTTATCTACTAAGTTTGCTTTAGTTACGGGATACTTCTATGCTAATAAAGAATGATGAGGATTTATAGTTATGTTTTTAACTTATTTAGTCTTTGTTATTCCACCAATTTTAATGATGAAATTAGCAATGAAATATTCAAAAAAATTTGAAAATAATAAATATTTAATAAAATTAATTAACATAATGAATCCTGTTGTAACAGGTATTATTGTTGCCTTGGGTTTTCAACTTTTTATAGCTTCAGTAGCACCTCAAGTTTTCTTTAATAAATCATTTACTAACTATGTAGGAATAAATAATGATACACAAAAAGCACTGTTTTATAGGGGTTGACGTCGAATTGCTGTTTACTGTTATGTGCCTGTTGGAGTGATACTTTCAACTACTCTTTATCTTAAAAAAGTCCCTGTGGTCGCTTTGATCTTAAGCAACATAGTTTTAGCTTTAATTATTTTTGAACCTTGACTAGGTTAAAAACAATTTTGAATTTTTAATTATAATATATAATTACTCTATGTTAAAAATTAGTGTTCAATATAAAGAGAGAATCGATAAGTATATCGGTCAAAATAGTGAATTAAGTCGAAATGATATTAAAGAGTTAATTGAGCAAGGTGCAGTTTATATTAATGACAACGTTCAAGTACATAAATGTAATTACATCGTTCGCGAAGGACAAGAAATTACAATTGTTAAATTACTTAACAAAGAAATTAAAATTGAACCAGTTAATATTCCTCTTGACATTAAATATGAAGATGATGATTTAGTAATAATTAATAAACCAAGTAATCTAGTTGTCCACCCTTCACCGGGGCATTACAATGACACACTAGTTAATGGACTTTTATATCATTTTAAAAACAATTTATCAAATGAAAATGGGTTACTTCGACCAGGTATAGTACACCGTATTGATAAAGACACAAGTGGCTTATTAATAATTGCTAAAAATAACAAGATGCATAACTCATTAACTGACATGCTCAAAAGACATGAAATTGAAAGAAGTTATTTAGCTATCGTTAGAGGCTTAATTGAAAATCCACTTACTAAAATTAATTTACCAATTGCTAGAAATACTGTTGATCGTAAAATGATGTCAGTTCATAAAGAAGGTAAAGAAGCTATAACCTTTGTTCATTTGCTTAAGCACTTTTATTTAGATAAAGAACCAATGTCTTTAGTTAGATGCGAATTAAAAACAGGTCGAACTCATCAAATTAGAGTCCACTTGTCTTACATCAAACATCCAGTTTACGGTGATCCTACTTATGGCAAAAGTGTTGATGAATTTAATCAAAGATTACATGCTTATAAATTAAAATTTATTCATCCACTTACTAAAAAAGAAATTTGTGTTTATGCACCGGTGCCTAAAGAATTTGATGTAGCCGATTATGATTTTAATGAACTATTAAAAAATAATGTTTTATAATATATTAATTAGATATAGGAGAAAAAATGAATTTATTCGACAAAAAATATTTACAAATTAAAAGCACAAATAAACTTTGAGCTCTAGAAAAAGAAAGATTTAGACCAGTTGTAATTCTTTCTCTTTTAACATTCTTTTTAGCACTTGCTTGTGCTTTAACTTCAACAATTTTGCTCGCTGCTTTTAAAGATAATTTAGTTGACTATATTGTAAAAATTAATGGTGGTACAACTCCAAGTTTTAAACCTGAAGATCAAATAATGAGCATGATTATTACAAATGGTTTAGTTACAGTTGGTATGTTAGTTAGCATTATTGTATTTTTAGTTTTTTATGCTCGAACATTTAAAGAAAAATCATTAGCCAACATGCATACATGACCTTCAATGGTTTTAATCATTTCTTGATTTATAAACTTGTTCTCAATTATTAGAAACATTAGATCACTAGCTACTGCTTACAATCCAATTATTTTATCAATAACAATAGTTACTTCAGTTATCACAGTTGTTTTAATTGTTTTCCAATTCTTGTTTGTTAGTCAATTAAGATACTTCAAAGGTGTATTTATTAATGCAAGAAGATTAGAAGAAATCAAACAACTTCAAAAAAAGATGAAAGAATCAGGTATGATGGATGAAAGCAATTTAGCAAACAATCTTTTTGGAATCATGTTAGATGATGTTGATGATGTAAGTGCTAATCAAGATAAAAGCAAATTCAATAAAGAAGAAGATATTGAAGAAGCTGAAATAGTTGATAACAAAGCACATCTTAAAAATAATGAAAAAGAAAATGAAGAAAAAATCAAAAAATTATTAGCATTGCCTAATGCAAAACTTTACAACATAGCAGAAACCCTATACATTAGTGGTTACAAAAAAATGGACAAAGAAGAATTATGCCGCTTAATTGTTCAAATTGTTGATACTCAAAAGGCTAAAGAAGACGAAGAAGCAAACAAAGACAAAAAATAATTTCAATACAAAAAGCAGAATTCAAGTTTTGCTTTTTTTGTATAATTTTGATTATGTTGAATTATGAAAAAGAAAATTATCCTGACACTAAATTAATTGTAGGCTTAGATGAAGCAGGAAGAGGTTGTTGTGCAGGGCCTTTAGTAGTTGGTTGTGTTATTATGCCTCAAAATTTTAACCATCCTTTAATAAAGGATTCTAAACAATTGAGTCAAAAACAACGTGAGGAAGCGTTAGAAATTATTCTTGAAAATGCTATTGAAACAGTAACAACTATTATTAATGCTCAAGAAGTAGATCAACTAAATCCAAAGCAAGCTTCAAGAATTGGCATGCAAAGGTGTATTAATAAACTTAATACAAAACCTGAATTAGTAATTACAGATTTTGAAAAAATTAATGACATCAATATACCTCAAATTAATTTAGTAAAAGGTGACCAATTAAGTATTAATGTGGCAGCAGCTTCAATTTTAGCTAAAGTAACTAGGGACTTAATTATGGTTGGAATTGACAAAAAATTTCCTATGTATAATTTCAAGAAAAATAAGGGCTATTGTACAAAAGAACATGAAGCAGCAATGAGTCAATATGGCATCTGTGAATTTCATAGAAAGTCATATAAAAATGTTAAAAAATATTTGAATTAAACAAATTAATTTTTTACCAAAACCATTGTTTTATGCTATATTTAATAAGAAATTGAGTTAATTATAAAAGGAGTTAATATGAAAGCTTTAGTAAAATCAAGTATTACAACTTTGGATAAAAATTATCCTCAATTTGATTGAATTAAATTAACAACGAATGAACAAATTAAACACTCAAAACCAATTTCAAAACCTATGCTAATTGCTCTAAGTGGCAAAACAGTTTTAAATGAAAAATTTAATGAAATTGTTCAACAAAAAATTGAATTAGAAAAGAAATATGGAGCATTTATTTACTTTGATGTAATTGTTAAAGAAGAAACTTTAACCTTAGATAATTGTGACTACCAAGTTATCATGGAAACATTCAAACCAAATGAAATTGCTAAAATTAATTTAGTTCCTAAAGACTATATTCCAGCTTTTGATCCTTTACCAATTAATAAATTAGAAGAATATGCAATGGAATATAATCCGGGCGGTTCAAAAAATTTAGCAAAAGAAGAATTAAATGAAATTAAAAGATGATATAAGCATGATGTTTATGCAAAATTCTTTAGAGAAATAAGAGATAAAGCAATAGAAACTTTTGATATTATGGTTTCAGACCATGCTATTGAAAGATGAAATAAAGTTAATAATAATGACTATGAAACAGACATAAAAAAGATAGCTTATATTTCAAAAATAAACTTAGAATTAGAAGCAATTAAAATGAATGAAATCAATTTAGTATCAAGATTTATTGATAAAAAAATCAAACTTGAACACTTGTCTTTTGAAAATGAAGTAGAAAAACATGATGCATTAAGACAATGAATTATGGAGGTAATTTATGGTAAAAAAATTATGTCTAAATTCTTCCCTGCTTTATTTAGATCAACTATTACATGATAGTAAACTAAATTGAGTTTAATAATAAACCAGGACAAAAAAATTAGACTTAAATCCCAAAAATATTTAAGTCTTTTTGTACTAGTTTAGGTTTTATTTTTTGCTTTGGAGTTAATAAAAAAAGACACCTTTTAAAAAGATGTCAATTTGTTTTTAGTAATAACTGCTTGATGTTGAAGCAATTGATACAGAAGATCTAATTGTTACACCTAAGGCAACTAAAAAACATATTATAAATATAGAAATAAAAATACCAACAATAGTTCATATAATGACTTTAGCTGTTTTCTTTTTGTTATTAATTCTTTTTATTCTTTGTTCATATTCGACTTTGTTCTGTCTATCTAATTCAACAGTTTTTTTGTCTCAGAAGTCATAAGAACAAATAGCAAAAACTTCAATGTTTCTATCTGAGTTTATTTTGTCGAGTAGGTCGCTGTCACTTAGCATAATTCCTTCTTTATTAAATACTCTATCTTTTAATACTTTAAGATAATCATTGAATTCATATAAACTCATTTTTCTTTCTAAGGCTTGTAATTCACTTCTTTTTAAACAACAAATATCTTTACCGGAATATTTCTTAATTTTTTCTGAGTAAGTTTTTAAGATTTCATCTTTGTCAAGTGATTCATAGTAATCGATTTTATTTGCTAAAACATAACTTTTCTCAAGTTCTGAAATGCTTCCTCCTTTGTTTACATATGTTTTCATTGAGTTTGCAGCTGTGTTAGAAGATAAATCAAAATCAATTTCGTCATATTTATCTTCTATAACTAATAAGTTCAATACATCATAATTAATTGCTTTTGATTCTGTTACTACTCTTGTAGTTGCAGTTGTGTGTTGAGATGTTTCAACGCTTGATTTAGGTGCTTCGATTAATGATCGGCAAAAAGAACAAAATTTATCTGTGATTCTAACATTAGCTCCACATGATTTGCATTCCATAATTAGTACAACCCTTCTTCATCTTTAGGTTTTTGTCCTGTGATGTATTCTTCTCTAAGTGATCTTTCTCTTGATTTAAATTTTTGATCTGTTTTACTCATTTTGTTGCTTATAAATGTTAAAGTAGCATATGGAACGATAAAGATAATAAGTGATACAACAAAGTAAAGTGATAATCAAATGTTGATATTGTTGAAATTAGGTAAGTAACCAAAAAATCTTAATATAAATGGCACAACTTGATAAAGGGCCACTATTACCATTAATCTATAGTAGAATGATCTAGCTATTTTGAAGAATGTTGTGTGTACAAAACCGAAAATTAAAAACAATAAAGCGAAGCATTTAACACCAATATATCAATACAACATGTCGGTGCTTTTGTCATGAAATGGAAATTTCTTAACACCAATTGGAATTAAAATCATTGTGATGAATATTAATCCAAAAGCGAGCATTCATGGTCATCTTTTTTTAATAGTTTCACGATCATTAAAGATTGTTGTTTTAGGTTCAACTAATTCTTGTTGTTGATTGTTGTTATTCATTTTATTTTACCTTCTTTCCGCAATTTACGCAGAATTTTGCATTTGGCGATAAGTCGATATTACATGATGAACATTTGTTTGTTGCGATTTTTGAACCACATTCAAAACAAAATTTAGAATCTTTAGATACTTTGCTTCCACATGTACCGCAAGTGATTAATAATGCAGCTGCTGCTGTTGGGGTTGCTGATTTATTGATTTTGCTATCACTTAATAAATTTGAAGCACCTTCGTTTAGTCCTAAACCAATGCCTGCTCCAAAAAAAGTTCCTACTGAACCCGAATTGTTTGCTGCATCTTGGTAAACGTCATAGCCTCTAACTTTAGCATAGTCGTCTCCCATGATATCAACTTCGGCTTTTCTTTTAAGAATTTTGTTCAATTCATCTTGTTGCTCTTTAGGTATACTAATATCTTCAATGCTTGAGTTAACAACTTCCATACCTAATTTTTGTGTTTCAGATTTTAAAGCTTCTTTGAAATCTTCATTAATATTATCTATTGATGCGATTATTTCATCAAAAGGTATTTTGTGTTCAAGAATGAATTTTGAAATAATTTTTTTAATGTTTTGATTAATTCAGCCTCTTAAGATTCTGTCTATGAATTCGTAATAAATTGTTCTACTTGAATCAAATGATCCATTTAATGTTCTATATAAGTATTCATATTTAGTAATTCTTAAACCAAATTGACCTCTTGCTCTTAAATATAAAAGCAATCCATATTTAGGGTCTCTAAGTGGAATAGGCGAATTTGTTCCTCAAAGAAAGTCTAATTTTAATCTTGTGTTAACGAAATAAAATTCAATTGGATATGGGTTTTTATTTCAAAATTCTTTTGTCTTATTTTTTAATATAGGGAATCAATCGCTATCAATATTACTTGTTCCTGGTTCAACAAGCTTAATCATTTGTCCGCTTTGAACAATTATGGCAACTTGACCTGGTCTAACGATTAATTTTGAATTGTTATTTACTTCACACTCTGGATGTTTTCAAATTAATCAATTTTGTGTGCCAATCCCTTGATTTTCTATAACTTGATAAAATTTTGCTGACATTTATTCCTCCTATTATGTTACTTTATAATTTTATTACAATTTTTGCAAAAAATGGTTAAAAATGACAGAAAAATCATTTATTTTTTTTACATAACACTATATATGTTTGAATATAATTCAATTTATTCAAAAAAATTAAAAATTAAAGAAAGCATTAAATATAAGATTAATAACTTTCGAATAAAAAATGAACTCTATTCCAATTAATAGAATAAAGTTCATTAATAGCTTGAAATTATGAGCTGTGTTTGTCTTTTGGACCTTCATCAGTTTCGATTCTTGGTCCTCCGTTTACAGGTGTAGATGAAAGACTTCAATCGCTAATAATGTATCCTTGACCATCTATTTGTGCGCCAGTAATAGCTAGAATAAAGTCGACAAGATATCATACTCCTAATCCACCAACTGTTACCAATTTTAAAAATCCAAGAACCACTTCACAAGAGTAGAATCTATCAACTCCACATCATCCAAGGAAGAATGAAAGAATAGTTAAAACTGTTCTTGATTTTGTAGATGTCATTTTTTCTCCCTTTATTTTTATATTTTTTTTGTGTTAATTTATTATATATCAATACAAAATCAAAACAACTAAGTTTTGAAAAATTTACCTATAAATAAGCCGTATCTAAAATTTATTAGTGAAAAAAATTATATGTATCCATGTTAAATAAATAAAGAAATCTCTTAAATATTTTTTTTGTGTTTATTCTTTTTATTTTAAGAACTCTAAAACTTTTTTGTGAATATTATCTAAGCTAAATCCTTTGATTTTGTACACTTCAAAACCATCAGCTGAATAACCATAGCCATCAGCTAAAAAGGCATCAATTTTGTTATATTTTCCAAGTTTATATCACAATGGATCTGTTGAAGCTTCAATAGCATATATTGGTTTTTTGTCAATGCCTAAGCTTTTGATTAATTTTTCATCATTAACTAAATCTTGTAATAAAGGAACGGAAATAACTTGAGCTTTAATGCCTGCTCCAACTAATTTATTAGCAACCTTTTCTGCTAAGTCAACTTCACTACCAGAAGCCAAAATACTAATGTCATGGTTTTTTGCAGGACGAATAATGTAAGCTGGTTGCAATTCACCTTCAGGATGTAAATTGTAAGATTTAACATTTTGACGACAACCAATAATAGCTACTTGATTTTCATTTTGATTTAATGCATAATTGAATGCTGCTTTGACTTCGCTCTCATCCATAGGTCTAATAACTTTAAAATTTGGAGTACTTCTAAGCATTGCTAATTGTTCTACTGGTTGATGAGTTGGTCCGTCACCCCCAACTTGGTAACTATCATGAGTGTAAACATGAACTGAAGGTATTTTCATAATTGAACCTAGTCTTAGAGCACCTTTAGCATAATCGCTAAAAGCTAAGAATGTTGAATCTATTGTTCTTAAATGTGAGTGTAAATAGATTCCATTATTTATAGCAGTCATAGCAAATTCTCTAATTCCATAGTGAATGTTTTTGCCACCTTCAGCATAAGATTTGCTAAAGTTAACTTTTGTAGCTGAGGCTAAGTCAGCTGAACCACCTAAAACGTTTTTGTAATTTTGATCTATTTTTTGCATTATAGGAGCAAAATAGTTTCTAGTTGCTGCATTACTTTCATTAAATTCAATATCATCAAAATTGTATTTTTCTTTTTTATTATATAAATCTTCTAATTCTTTAGCTTCTTGAGAATATTCTTTTTTATATTTATCATAAAGATGTAATCATTCTTTATAATGTTTGTCTTTTTCTTTTAATAATTCTTCTCCATAGTCATAAACTTCTTTGTCATATTCAAAAGGAACAAATGAAGTTAAACCACATTTCTTTTTAAATTCAATAGTATCTTCTTCTTTTAACATTCCATGGTGACCTTTTGGCGAGTTTTCAAAAGGTGTATTTTTAGCTATTGCTGTGTGAACTTGAATGTATGAAGGTTTCTTAGAAGCTTTAGCTTTTTGAATTGCTTCAAGAATTAATTCAGGTTTATCTTCTTTAACTACAAAGGTTGCAAAATGACAAGCTTCAAAATATTTAATGAAGTTAATATTATTAACAACCTCGGTTTTTGTATCAATTTGAGCATTATTAAAATCATGAATTAAAATAAGTCTATCTAATTGATTTGTACCTGCAAATTGAATAGCTTCATGAGCCACACCTTCTTGAATACAACCGTCTACATGAAGCGCATAAATGTAGTGGTCTATAATATTGTAATTTTTACGATTAAACCTGTTTTGTAGTACTTTTAGACTAAGAGCCATACCAGTAGCCATAGCTATTCCTTGACCTAAAGGACCTGTTGAAGCATCCACAAAAGGTGTATATTCTAACTCAGGATGAGAAGGTGTTTTTGAGTCCATTTTTTTATGATTTTGCATGTCTTCTTTTGCTAATAAACCTAAGAAATGCATAATTGAATAATATGACATTGAACCATGTCCAGCTGATAAAACAAAGCGATCGCGATTGATTCATTTCGGATCTTCTTTTGAGAATCTTAATTCCTTTCCTAATAATGAATAAGTAATATTTGCAGCTCCAATAGCCATACCAATGTGGCCACCTTTTGCTTTGTTGATTGAATCCAATGCTATGCCGCGCATTGAAGCAATTAATTTATTTTCGATTAAATTATTTTTCATAAGTCTCCTAAAATTTTATATATCTATTATAAATTAAATAAAGCCTTTTTTATTAAGAAAAAAGAGCCTAAGCCCTTCTATTTATATGCCTTAATTAATCAATCATATAGTTCTTGTTGATTTTTTACGCAACCATCTGATACATAAATCATTCCGTTGATAAATCTGTTTAATCTAATGTCGCGATCTGCTACATCTCTAAAATCTGACATTCAAACACATATTTTAACTTCTGGATCTCTTTCTTTCATTGCTAAGAAGTAACCAAATTCTGCCATGGTGCCATCATCTTTGTTGTCAATGTCAAGAATTGTAAGTTTACTTTTTTGAATTGCTGCTAAGTCATTTTTGAAAATTTCTTGTGGTGTTAATTTTCCGTTTTGTTCTAATTCTTCATTTAGATGAATCGGATTGAAAATGTCAAAGTCAATTTTGCCTTTTAATTCTTTTGATTTTAGTGCTTTTTTCATTGTTTCTTCGATTGCGTATCTTAAGTCAATTTCTGCTCTAGTAAACAATGGACCAGCTACATAAGCATGTATTTTTTTCATATTATTCTCCTATTTTTTTTATGTTTATTTCTTTATTATATTTTAAAAAGTCGACTCTTAAAATATTTAATAAATTATTTTAAAAAAGAGTAAAAATAAAACCAGAATGATCTGGTTTTATGATTATTTTTAAACGGTTGCATTTGCATATTTTCTTCTATAATATGCAGATTTTTTGAAGAAGTGAATTGAAATTGTTTGGATGATATTTCAAAGCCCACTGAAGATTCAATACACTTGGACACCACAACTAAACATTAAAGTAATGAATAAGAATACAATCATAATAATGTTTTGTGTCTTATTACTTTTCTTCAATGCTTTTTCTTCTTCGATAGTTGTTCTTTCTTTGAATTTTTTCCTGTTCAGTAACCGTGGAGTAAATTGCGAGAAGAATTGAACTGCTAAAGCAACAACTAATATTCCAAGGTAAGCTCATTCACCAGCAAATAAACGTTTTCAGCTAGTTGCAGCAAAGTCCATACCTAGTCAATGAGTTGATTTCATTTCTGGAATACTTTGAATAACCCTTCACATAGCAATGAAGATAGGGAAGGAAATAATTATATTCAAGAAAGCATCAAGAGGATTTATATTATTCTTTTTATATAAAGCCGCAATTTCTTGTTGATGTCTTATTTTCATTTGTTTATTGCCTTTGAAGTCAGCATATTTAGCATCAATTTTTGCTTTTTTAGCTTTAATATCTTCTTGTTTAGATTGATTAACTTGAGATTTTCAAGTAATTGCTAAAGCAAGCAGACGAGTTATAACAACAGCAATAATAATAACAAATATTGTACTTCAACCAGCAAGTGAAGGCATATTTTCACGCATTGATCCTGTTAATCAAGCAATAGGGTGAACGAATAATCCAAAGAATGGACCTCAGCGTTTTCATGAATCACCTCATTTAACAAAAGGTTTGAATGGTTCACTATGAGTGTATACAAGTTTTGGAGCAGGAGAAAGATAAGCATTTTCTTTAGCTATTTTAACTTTTTCAGTTCCTTTAGCTAATTCAACTTTTGTTAATTGAGTGTCGTCATAAATATTTGTGAAACCTGTAGTTTTAAGATATCCATTTAATATATTTACATATTCATAAATAAGTTCCACTTGTTTTTTACTATATTTGTTAGTATTTGTAGGATCAATTATTTTGCCTAATACATCATTGTATTTATCGGTACCAAACTCTTGTTTGAATCTTCTAGCAAAATAATTATCTTTACCATTTACTTGTTCAATAAATGTTTTTCTATATAAAAATTCTAAAACATCACGAGCATAGTATTGTTTTGAAAGCTCAATTTCACTAAGTTCCTTACCAGCAATTTTATTTTTTGCTTTCAATTGGTCTGGTGTAGCTATTGAAAACAATGTTGCTTTTTTAGGGGTTTTGAATGTTTTATTTTCTAAACGATCTTTTTTAACTTCATAAAGATCTGGATTTTTTTGATCAACTAAAGATAAAATTGAATTTAAATTGAATTGTGGGTCAATAAATTTAATTTCACTATAAGGTGTTGTGTAGGTAGGTTTATATGTTGCAGACTCGCTCATATCAAAAAGATATTTACCATTTTCACCTTTAACTATTGCAGAATCGTAATAGTTTGCTTTTCATTTACCATCAGCATTATGAGTGTCATAGAATCATTGAATAGAAGATGAATATGAGTTATATTTTCCATAAACATCTCCGCCTGTTTGCTCTCTTAACTTTTTAAGTATTTCGGCATGATTTTTGTAGCTAGCATGGTAGTTTGTAGAATCATTTTTAACAATTCTCTTGAATTCACCAGGTTGTAAAGCCTTGTCATCTTTGTCGATTTTTTTATCAGCTTTAGCTGGTTCAAGTGATGTTACATAAGGACTAATTTTATCGTATGATGTGTAATATTCAGTACCTGCACCTGTATATGTTGAGGTCTTGATAACCATAGTCTGAATACATCCTGTCATAGTTAAACCAAATAACAAGGTGTATAAAACTATTTTGGTTCAGTATCATATTTTCTTTCAGAGATTCTTTTTCTTTTCTTTTTCGGAATTAGAATCTGAAAAATAGTTGAATTTGTCTGATCTATTTTTGTTTTCCATTTTTAATCTTTTCAAATAATTTTTTAGTTATAGTCAATTTATCTTCATATATTAAATTCAAAAAATCTTTTCTTGGAATTAAGACAAAATGCATTTTTTCGCCATACAAATTCAAAGAATGGATAATTGATTTTAATTGGCGTTTATAGTAATTTCTTAAAACTGCATTGCAAAACTTTTTCGGTACAGTTATACCAATTTTGAAATCATCGTTAGGCATGTAATAAATAATCATGTATTTATTTAGAACTTGCTTTTGAAATTTGATGACAGCGTCAAATTCTCAACTTTTTTGAAGACGATAAATTTTTTTCATTTTATAAACTAACTAATTATTTAGCATCTGAAACTGTAAGTCTTGCTCTACCTTTAGCTCTTCTAGCAGCTAAAACTTTTCTACCATTTGTTGTTGCCATTCTTGCTCTAAAACCATGAGTCTTGATGTGTTGGGTCTTGTTTGGTTGATATGTTCTTAAACTCATATTTCCTCCTTAAATTATTTTTTTATGCATGCATTTTATCAAATATTAATATTAATACTTAAATCTCAATAAAATGTGCCTTAATTATATATTAATTATTAAAAATGCAAAAGAAATTATTGTCTTAAATCATTTTTGTAAAAAAGTATTAAAAATTTAATATTGTTGTATTATTTAAGTAAGATAATTTTATGAAATTAAGGAAGAATTTTTAAGGTTATTTTTTGATTTATAAGCCTATAAAATAGGCGGAAAGGCAAAATGCTAAATTTAAAATACGTTTTAGAAAATGAAAAGAAAGTTAAAGAAGCTTTAAAAAATAGAAATTTCGATCTAAAGCAATTTAATGAGCTTATTAAAGAAGCAAATAAGCGTGGAAAGCTAATGTTTGAAGCTCAAAGCAAGAAAGCTGAATTAACAAAATTTAGTAAAGAATTTGCAGCTAACAAAAACAATAAAACTAAATTAGCTAAATTACAAAAAGAAGTAGCAAATATTAAAAAAGAACAAGTAGCACTTGAAGAAGCTGCTAAAAATATTGAAAAGAAAGTTAATGATATTTTAATCAGAATACCAAATATTTCAATGGAAGATGTGCCACTTGGTAAAGACGAAAACCAAAATAAAGTTATAAAAAATCATGATAAATTAGGACGTGGATTAGTTTCAGGCGTTCAACCTCACTATGTTATAGCTAAAGACTTAGACATTATCGATCTTGAAAGAGCAGTAAAAATGTCAGGTTCAAGATTTGTAATTTACAAAGGTTTAGGTGCTAGATTAGCTAGAGCTATTATGAATTTTATGTTAGATTTACATACCAAAAATGGTTATGAAGAATATTCGGTACCTGTAATAGTTAAACCTGAAATTCTTTTTGGTACAGGTCAATTGCCTAAATTTAAAGAAGATCTTTTTGCTTTAGAAGATGCTCAAAGTTATTTAATTCCAACAGCTGAAGTCCCTTTGACAAACTACTATAATAATGAAATAATAGATTTATCAAAACCTATTAGAATGACAGCATTTACTGAATGTTTTAGATCAGAAGCAGGAAGTAGTGGAAAAGATACAAGAGGTATTATTAGAAACCACCAATTCAAAAAAGTTGAATTAGTAAAAATTGTTAGTGAAAAAGATTGAGAAGCTGAATTTAATCAAATGCTTGAACAAGCTAAATCGGTTTTAGAAGAATTAGAAATACCTTATAGAGAATTATTATTATGTACAGGTGACTTAGGATTCTCAAGTAGAAAAACTATTGACTTAGAAGTTTGATTGCCTTCAGAAATTAAGTATCGTGAAATATCATCAGTTTCATATATGGGAGATTTCCAAGCACGTCGTGCAATGATTAGATATAAGAATGAAAATGGTGAAAATGTTTATGCTCATACCATGAATGGTTCAGGTCTTGCAATTGATAGATTAATTGCAGCAATTCTCGAAAATTATCAAAACAAAGATGGTTCAATTACTATACCTAAGAAATTAGTTCCTTATATGGACGGAATAACAAAAATTACAAAATAATAATAAGAAAAACCAGGCATTTGAAGTGTCTGGCTTTTTGTTTAATTTACCTATAAAACAGATTGATTAGAAAAATCTATTCATTTTGATTTGCTTTATTTTCCAATTCTAAAATGCGTCTTTTTTCTTCATCATCATCTACTGAAGTTCGTTTAATATCTCTAAGTTTTAGAGCTTTAAATAAGTTAGAATTTTCATTTCTTTCATTGAAAATATATTGTCTAATATTTGCATAAACTTCATCAGGTGAAATTAATTTTTGTCAAGATGTTTCATTTAAATTAATCATAGCATTTAAAACATCTTCATCTTGATCTAAATCTGGCATTTTTAAGAAGTAATCATAGTTACTATTTTTAAATTTTTTAATATGATTTTCAACTAATAATCAGTCTTCTTCTTTATCAAAAATTTTGAAAAATTGTGCACCATAATAAGCTCTAAAACCAGGTGTTTCAAATGAATGTTCTTTATCATAGCATTCATTAAAAAAGTTAACATTTTTGTGATAGTCATTATCTAAAAATCATAAGATACCTTTGATTTTATTATTAGGATAAATTATTCTTAATACTTCTAATTGCTTAATGATATTGTCATGAAGTTTAGTAACTTCTTTAGCTCCATAGTAATCTCTTTTTTTCAAGTAAATTACACAAATATCAGCTGTTTCTTCATCTCCGCCATTTTCTTTTTGTCTAAAAATCATAGCTGCATTGACTTTTACTTCTTCTGGGAATTCTTCTCCCTCTTTAAGTGGTAAAGTATATTTAAAAGAACTTTTTTCTAATTTAAAACCATATTGAGTAATTAAATATTCAGCACAATATTTAATAAATTTTACATATGTGTTTTCTTGTGTTCTTAAGAATGCTTGTTCTAATTTAACTTTGTAATTAAAAGGTTGCAAAGGTGAAAAAAATCTATAAGGTGCATCAATTATTTTGTATAAAAGTTTTGTACCATTGCCTGTTGTTAAAGCACTTCTAAGTTCCATTTCAAATTGATTGTATTCCATAATAATCTCCTAATCAACGTTTTGTTCTAGTTCAAGAAGAATATCTTCTTCTTTTGTTTTTTCTTCATCAAATTCTAAGAATTGATTCATTTTAATATTCATAAATTTTTGTCAAAAGTGTCTTTTTTTGCTTTCAATCAATGCATTAAAAAATCTTGCAATTCTTTTTGGTTCATCTTTAATTGGCGAGTGTCCCGTACCCATAATTAAAATTGGAACTTCTAAACTAATTGCATAATCTTCAAAATATTTAGTACCAATAATTTTGTCCTTTTCACCTACTAAAAAGAGCATTTTGTGACAATTTGCTTTGTATTGTAAATCTAATTGTTCCATATATTCAGGATTCAAAACATATTTTTCAAGTAAGTTAAATCAAGCTCCTTGTCGAGTAAAACTTTCAATTAATTTTTCAGTTTTTTTAAACTTAGAAGCTATTTTAGAAATAACTTTGCCAATAAATGAGCTTCATTTATTTTGAGGTTTAATAACAGATTGTAACACTGAATAAGAAGTAGATTCTTTCATATAAGGATTAATTGTTGACATCATAATTAATCTAACAACTTTAGGATCCTTAGCTAAAACAGTAGCCACGCCACCTGCCATTGAGTGAGCTACTATGACCACTTTTTTAGTTTTAATTCTATTTAAAACCTCGTTAGCTACTTCAATTCATCACTCAAGTTTAATTTCTTCTGGTTCTACATTTTTGAAAAATTTACTTCCTGGAAAATTAATAGCCACAACATTGTAATTATGTGTGTAATCTAATAATGGTCTCATGTATTTTCAGTTTGAATTAAGTCCATGAAGAAACAAAAAAGTTGTATTTTTTTTGTTATCTTCTATATATAAAGGAACTTTGTCGCCATTGTATTCAATATAGTTTAATTTCATAGTATTTATTATTATATTAAATATTACTTTTATTTTTGAAATAAAAAAACAGCCGCAGCTGTAATTTTAGAATTTGTTTTCAGGAAGATATATTTTTCCATCAACACTAGCAACATTACTAATAGTAATCAGCGAGTTTTGATCAACGGCCCGAACTTTCTTAATAAGACGTGGCACTTGATTGTAAAGAGTAATTGATGAAAGAACTTTGGTGTTATTTCCTGAGTAACCTCCAACGCCTTTAGTGATAGTAAAGCTATTAACAGTTTTATTATCACTAATAATTGCTTCTCTAATAGCACTCATGTGAGGCGAGTAAATTTTAACTTGAACAATTTTGTAACGAGGGAATAATTTGTTAAGGTACATAACAAAAATAATGTTACAAATAAATGTTGATATAAAGTTAGGCGAGAAGTAAAGCGATGGTTTTCAAGGTAACGTTTTTAGAGATTTAAGAATTTCTTCAACTTTTTCTACTGTTGTATTTTCTCAAGTCACTGAATCTGCTTTAAAACCATTAATGTCATTTAATAATAAACTTCCTGGTAAGTATGTACCAAGTACAACTGAAACAATAATAATTACTAAGTTGATATAACCATTAATTGTTCCAAAGTTTTTTTGTTTAACGACAGCCATATATTCGCCAATAATTCCAGTAACTCCTGCACTACCTCCTATTATTGCAATAATTGCAAAGAAGAAAGCAAGCATTCAACCATAAGCAACAGAAAAAATTATTTGAGCAATTATGTTACCTCCATCATTTCATTGAAGAGGAACTAGTGATCATAATTGTTCTTTTCCTTGTCAATATTTGTCTGCTACTGCTTTTCTTAATTCTTCTGGTATATCACTGTGTGAAAAGTTACCAATAATATAAACTGAGTTAGCCCCTGGTATTTGACCTAAACCAAATGAAACTAAACTACTTACAACTAGAAATTCTAGTGTTAATATAGTAAAAACTTTACCGACTTTTTTTCATCCAAATATAAAAATTGGAATACTCAAAACAAGATAAAGAATTCAGAACAAGGCGTGGTCAATAACGTTATAAATAGTGTCATTAATGTTTGGGTTTGATCTTAAAAGAACGTTAGTCAATCTTGAAATTGCTTGTCCAAAAGCTGCTAAACCAAAGTTATAAATTCCTGGGTTTTTAACAAAGAAAACTCCGACAATACCAAAAACAATTGCAAAACCAGCTGTGATTAGACCTAGTTTTCAAAGAGGCATAGGAGCATAAAAACGAGATAATTTCAACACAAAATTCGACATTTTTGTGTATTTGTATTGAGTATTTTTTTTGTTAACAACGTCATCTAAATGAATTGAATTCATTTCATGATGTTCTTCAACATGCTTGTCTTCGACTCTACCATTATTAAGTAAAACGTCTCTTTCGAATGCATTAACCTCGTCCATCACTTCTTTGTCTTTTTGTTTTTGTGTATGTTCTTCATTTACATTATGTTCTTCGACTGATGATTGATTTAATTTTTCATCATTTTGTGATTTATTAGGCATAAACTCCTTTCTTTTAATTATTCAAAAATTTTAAAAAAACGCGCAAAAGCACTTATAGTATTTTACATTATTTTTTATTAAATTTATAAACCTTAATTAATTGTAATAATTGTAAGTGCAATTTTTTAGTTATTTTTAAATATTTTTAATTTTATTAAAATAGTCATTTTTTACGAAAAAGCCTATAGAATAGTCTTTTAATTAAATAAATAAAAAATTTTATTTATTGAAATTTATAAAAATAAAATTAAAAAAAATTTATGAAAAATTCCATAAAATTTTCACCTTTTTAAGGTATAATCTTTATATTAAAAAATATAAAGAATTGGAGGCAATATGTCTAAAGAATATGATGCATCGAATATTAAAGTTTTAGAAGGATTAGAAGCCGTTAGAGTTAGACCTGGTATGTACATCGGTACAACAGGAATTAGAGGTCTTCACCACCTTATTTGAGAAATAGTTGATAACTCTGTTGATGAGGCTATGGCAGGTTTTGCAAACAAAGTAGAAATCACGATTACAAAGGATGGTTACATTGATGTTATCGATAATGGTCGTGGTATTCCTACAGCAGTTCACCCTAAAACTGGTCTTTCAACAGTTGAAACAGTTTTAACGGTTTTACATGCTGGCGGTAAGTTTGACTCTGACTCATATAAAGTTTCTGGTGGACTTCACGGGGTTGGTGCTTCAGTAGTTAATGGACTTAGCGATGACTTTGAAGTTTGAGTTAAACGTGATGGTAAATTACACTATGCTCATTTTAACAATGGTGGAAAAATTCTTCAACATCTTACAATATTAAACGAAGTAGATCCTCAAGATACAGGAACAAGAATTAAATTCCATCCAGACTTTAGCATTATGGACAAAAACGAATGAGATAAAGGTGTTATTGTTGATCATGCTAAACAAATTGCATATCTTAACAAAGGCCTAAGAATTATTGTTAATGATGAAAGAGACAAATCAAGAGAAGAATATCACTTTGAAGGTGGCATTGTTGATTATGTTAAAGAATTAAATACAGGTCAAAAATTAATTCATCCCGATGTTATTTATGCTGAAGGTGAATACTCATTCCAAGATTCTCCAGCAGTTCAGGTTGAAGTAGCTGTTCAATATAATGAAAGAATTACAGGAAATGTAGTTTCTTATGCTAACAACATTATTACAGTTGAAGGTGGAACACACGAAAGCGGTTTTTATGATGCTTTAGTTCGTTTAGTAAATAATTATGGAACTGAATGTGGATTAATCAAAACTGAAGAAGACAAAGTTACTCGTGAAGACGTTAGAGAAGGTATTGTTGCTATTATCTCAGTTAAACATACAAACCCTATTTTCGAAGGCCAAACAAAAGGAAAATTAGGAAACAAAGATGCCAGAATGGCTGTTAACAAAGTGTTTTCAGATTCATTTGAACGTTTCTTAAATGAAAATCCTGCTGAAGCAAAAACTATTGTTCAAAAAACAATTTTTGCTAAAAAATCTCGTCTTGCTGGTTTAGCAGCTAGAGATGCAGCTAGAAGAAAAACTGTTTTTGATACAGGTTCACTTCCAGGAAAATTAGCTGACTGTTCTTCAAAAAATGCTGAAATTAGTGAGCTTTACATTGTCGAAGGTAACTCAGCTGGTGGTTCAGCTAAAATGGGAAGAAACAGAATGACTCAAGCTATCTTGCCTCTTCGTGGTAAAGTTATTAATGCTGAAAAGAATCAACCTAAAAAAGTGTTTGCAAATGCTGAAATTCTTTCATTAATTACAGCACTTGGAACAGGTGTCGGTGAAGAATTTAATATTAATAAATTAAGATATCACAAAATTATCATCATGACCGATGCCGATGTCGATGGTGCTCACATTAGAACATTGTTATTGACGTTCTTCTACCGTTACTTTAGACCTTTAATTGAATACGGATTTATTTATATTGCTCAACCTCCTTTATATAAAATAGCTCAAAACAAATTTATTGGATATGCTTACAACGATGCTCAAAAAGAAGAAATTATGCAAGGCTTAAATCAAAATCAAAAAGTAACAGTTCAACGTTACAAAGGACTTGGTGAAATGGACCCTGAACAACTTTGAGAAACAACTATGGATCCAGTTAATAGAAAAATGCTTCAAGTTCAAATTGAAGATGCAGCAAGAGCTGACTGAGCATTTACAACATTAATGGGAGATGAAGTGTTGCCTCGTCGTGAATTCATTGAACAAAACGCTAAATATGTAAAGAATATTGACATTTAATAATTATTTTAAGTACCACATTTGTGGTATTTTTATTTAACATAATTACATATACAAAAAATTTTTGATAAATTTTCTCATTGTTTTATTAATTCTAAAAGTGAGATCTCTAAAACCTAGAAGTCTCACTTTTAGAATTAATACTTATTATCTTTTAAAATATATTAAAAAAATATTTTAAAAAATAAACTTATTAACTTTTAATTTAACATAAGAATATATATAAATTTTAATTTAAAAAATATAATGTTAAAGGTCAAATCAAAAAATAACGATTTAGTTTTTGCACAGATTTTTAAATAAAAAACCTGTCGAAGACAGGTGTGGCATTCATAAAGAATAAATGGAGCGAACGAGGGGAATCGAACCCCCATTATCAGCATGGCAAGCTGGCATTTTACCATTAAATTACATTCGCGTTTTTGCTTTTAATATTATAAACACATTTTTTAAAAATCAAAACATTTTTTATTTTGAGTCAACTTTATTTAAAATTGAAGTATGAAAAGACATAAACCAAGTGAAATATTTATTAACAATTTAGGCATTTGAAAAATGAAGAAGAAAAGATCTATCAAAAGCTAATTAAAGAATATGAAATATTAGTAGCTTTAGAAAAATAAAAAAAGTCAATAGACACAACACTACTCGATATGGCTGCTTCATCTCTGACCTGACCAGGTTACAAGGTTATTGTTCTACTGACGCTACAATTATACCAAAAATTTATTTATTTCAAATTGTTTGACTAAGTCAGGCTTTTTTTATTGGTCTAAAGATCGTTTTTTGCTTATTCATATACACTATTTAAAAAAAATATAAATTTTTTTGTGGAAATTTTCACATTTTAATTTTTTCTATATAATTAATATATGAAAAATTTCTCATATAAAAATATTGCTAAAGAAGCAGGTGTCTCTATTAGTACTGTAAGCCGTTACTATCGTGGTGGTTATGTTAGCAAGGAAACTAAAAAAGTTATTGCTAATATCATAAAAGAAAACGATTATTATCCAAATCATGGAGCCAGAACAATTCGTGGCCGTGACACTTCTATCTTTATTATTGTGCCTGAGTGATATGAAAACTCATATACTCAAATTATGAATGGAATTGAACAAGGAGCAAAGACAAGAAATAGAAAAGTTGTTATAACTCACTCTTCACCTAATCCTGAAGAATATATTGAAACTATCAAATATGTTTCAAGCTGAAAACCTATGGCAATAGTTTTCTTTCTTCCTGAAGATCCTCAAAACACAATTACAAACTATATTAAGAACACAAATTTTGAATCAAATATTTTAGTTTATGGAAAAGAAGTAGAAACATTAAATTGAATAAAAATTGATGAAGAGAACTCATTTCATCATTTAACTTCAAGATTCATAAACTATATAGATAAAGGCGAAAAGGTTGTTTTTGTTGAAGATGTTAAGTTAACTGAAGAACAAAAAAGACTTCGTTGTATAGGTTTTGAAAATGCTTGCAAGAAGCTTAATGTAGAATATGAAATCTATTCACTTAAAAACAAAAATAATAAAGAAGTACAATTTTTCTTAAATTACTTAAGAAATAACAATTTGGTTAATGTTGTTTGCTCAACTCATGAAGTATTTATTAACTTAATTTCATCAGGCGATCGTAATTTAAGATTAACTGATATTGGTTGAGTTTCAATTTATGACTATCAACACAAATATAAAGCTAAAATCTTTATTGATTATCCTGCAGTTGGATTAGAAATTGAAAAAATGCTTTATAGTTGTGACGTTGATAACACAATTGAAAACAAAATTTATAAACCAAAAATTTTATTCGATAATTAAAAAATTAATGACGCACTTTAGCGTCATTTTAATATACATTTTTCAAGACTTTTTTGATGTATCTTTCATTATCTCTTTTTTTGATTTCTTCACGTTTATCTGTTTTTTTCATTCCTTGAACTAAAGCTATTTCAATCTTAATACGTGAGCGATTATCAAAATAAATTTTGCTTGGAATAATAGTTGCATGACCTAGCTTATGAAGTTTAGACTGCAATCTAACTATTTCATTTTTATGCATCAATAATTTGCGATCTCTAGTTTCATCACACTTAACTAACATGTATTGTTTGAACTGTGCATTGCACAAGAAAATTTCGCCTTTTCTAAAAAAGCAATATGAATTCAATAAACTAACAGTATTAGCTCGAGCACTCTTAACTTCTCAACCCATTAAAGATATGCCGGCTTCATATTTGTCAATTACTTTATAGTTGTGCATTCCGCGTTTGTTGTCGCTTATTATTTTCATAGTTTAAATATTATAAATAAAAAATAGGACTAGCCTATTTAATTATTTTATTTGTATTCCATAACTTTTGCTTTAAGGTGATCATAAGGTACATAGCCCATAACTTTTTCCATCATTTTTCCATTTTTAAAAATGAATCATGTTGGCATTGAATTAACACCCATTTCTTGAGTTAGCTTTGTATTTTCATCAACATCTACACGATAAACTGGAATGTTGTCTAATTTGTCTAATTGTTCTAAAGAAGATTTAAACATTCGACATGGAGGACATCATAATGCGTGAAAAACCAAAACATAAATGCCTTTTTCTTTTCCAATAACATTGTTCTTGTATTCATCTTGATTAATTTCTTTTAACATAAAATCTCCTTATTCATTTTTTTAAGTAACTTAATCTTAACATATTTTTAATCTTTTGTTAAAAAATAAAAAATTATTTTTTAACCTATTTTTTAATCAAAAATGCACTTTTTGTAATTTTTACCACATTTTAAAAATTAGTTAAAAATTTTGCAAAAATTAGTTTTGAAAAGAAAATATTCGTTTTTGTTTTTTGATTTTTCGAAGTCAAATATTAATCTTCAAATAAGTTTTAAATTTAATATTTGAGTTTTTTTAAATTTATTTTAAACCTAAAGCATAAAAAATAAGGCTTTATGAGCCTTATAATTTATTTTTTTTAAAACCATTTAATATAACTGTTTTTGAGTCTGAAACAGTTTTGAAAGGTATTTCTTTTTCAACATATTCATTCTTTTGTTGGTCATAAACTTTTTGTACATAACCAATTTTGTAACGTAATTTAAATGTAATAACAACTTCGCCTTTTTGATCATTAGCAACAATTATTGATGGTTTTTCAATTTCAAAAATATTGTTATCAAAGTTAGTAAATTTAATATCATTAATGTTTAATTGACTTGGCAATTTGCTTGAAGCTGTTGGACAAGTACAAGTAACCTTTTGAATTTCTTCATTGATATGTTGTGTCAATGATTTTTCTGAGAAGCCTGTAATTTTCTTTGTGAATGATTTAACAAATTTGAAACCGCGTTTTTTGTTGTCTGGTTTTAATTTAGAAAGTGCATCAACTTCATATAATTTAAATTCAACTAATATTGATTTATCTTTAAGATCAAATGAATCTATTTTTGTAACTTCAATCATGAAGTTTGAATCCATTGATTCAATAAATACATGATCTTTATTCGCTTCATTTGCAGCTACTAAGTTTTTACGTTTATAATTCAATTTAATTAAATCTAAAATTGCATCAATTTTAGATTGTGAAAGTTTAGAAAAGCCACTAATTGTTCTTGAATAAACTTGTTCAATTGAACTGCTTTTACTTTTTGCTTTGTATTCAATAACAATAGTTGAATTGTTGAATTCATAACCAATAATTTTTAAATCAGTAATTTCATAATTTGGGTTAGATAAAGTGTATTTAACTTTAGAAACTTCTGCCTTATCAACTTCTGTTGTTGCAGCATTTTCATATGTAAAGCTAATTTGTTTTTCAATTTCACTTAATTTTTCTTTACTATCTTTTTTGTTAAATTCTATTTCACATGCATAATCAAATTCAATTGTTTTTTTGTTTTCATCAACTAATAAAGTTGTTGCTTGTTTGTTAATAGGTGCTTTAATATTAAATTTAAGATTTACTTTGCCTTCTGAATCAGCAGGTTCAACTTTGATGTTTTCTATTGAAAAATCAATTGGTGTGTCAAAATTAAATTTAAATTTATCAGCCTTAACTTCAGATACAGAAACATTTGTATTTCCTGTATATTCTAGTGAAGCAGTTTTTTTAATGTATTTCTTTATTTCTTCAGTTAAATCTGAAACAGAGTGAAATGAATGCGAAACTGTTGAGTATGTGTCACTTTTATCAAAAACATAGTAATCAATGTATGAATCATTTTTGAAGTTTGGAATTGAACGACTGAATTCATCAGATACAATTTTAGGATCATTTATTGATTGAATTTTGTATTTTATATTTAAATTCTCGAATGATTTGATATATGAATCTTTATCCGCAGTATCTTCATATTTAGCTTTTCTAATTAAAGCGCGTTCATATTTTTCAATAATTTCCTTATTAGGATTTGCTTTAGCTTTTTCAGCATCATATTCAGATTTATTTTTTGTTAAGAATTCATTTATATCTTTTAGTTTGTCAGATAAATTTTTTTGATATTCTTCAATTCTTGAGTTATCAATTTTAACATCGATAATTTTGTATCCTGTTTTAATATCTCTAAAGATAATGTCACTTGTTTGAGCAACATCTTTTACATAAGTATTTTTTGAATTAGAATATTGTACTGTTAAAGCTTCATTAATATGATCAAAGTTTAAGAAATTTTTGTATGTAATTGTTTTGTATTTTGAAACAATGTTTGAGTTTTTTTCGCGTAGTCTAAAAGTTACTACAAGTTCTGAAGAATATTTTTGAATTTTTTGTATTTCAACAGTATATTCACTATTAAAACTTGGCATATTTTTTGAGTAAAATTTTAAATCCGATTCTTTGATTTCATTTGGTTTTTTAGATTCTGAATCTGGAATAGTAATGTCTAAATCATCTAATAGATTAGACAATTTATCAGCATTAGTACATGATGAAGAAATAAGAGGTGTAGCTAAAAAAGCTGTTGTTAATGATGATAAAGCAAATATAAATTTTCTTTTTTTCATAATAATCTCCTTATATAGCTAACGCTCTGATTTGTCATAAGGTCGACCTGCAGCAGCTGGCCCCATTTGCTTGCTTTTCATTTTAGCAATCATAATTCCAAGTGTGATAATAAATGGCAATGTGTAGAGTAGTTCAGCGAATCTTGAAACACCTTTCATAGATTCGAATCTTTCAAGTCATGAAATTCCGAAGTATGAGAATCCATAGATAAATGAGAAAATGATACATATCAATAATGTTCAACTAATTTTTCAACCACTAGTAATCATAATAGCTAGAGCTAAGAAACCTAAACCTCCAACTTCGATGTATAGTGAGAATTGGTTTGTTTTACATTGAATAAATATAGCGCCTGCTAAACCAGCAATAAAACCTGAATATGCAATACCTAATCATTTGTATTTAAAAACGTTAATACCAGCAACGTCTGCTGCTTGAGGATTTTCACCTATAGCTTTATATCTTAAACCCCATTTAGTTTTGTTAAGGAAGAATCAAGAACCACCAGCAATAATTAAAGCTAAGAATAGTTTAAGTGAAATAATGTTTTTTCAGTTGTCAACATTAGGACTTAAAGCAAGTTCTGTTTGGCCACGCATTTGGAAGTAGTCTCCACCTTGTTGTGCTTTTTTAACAAATAATAAAAGGATAGAAGCAACACCAACTGCAAGAATGTTCATAGCGAAACCTGAAACAGATTGATCTGATTTAAGTTTAATCGTTGCGAAACCAAACAACAGTGCAAATCCCGAGGATAAGATACCTGCAAAAGCAAAGTACAATAATTGGAATCATGGACTTAATGTTTTACCTTTGAAAACAACATCAGTCATTAGGAAGCTTATTAGAGCATACATAGCAGCTCCAAAAATCATAAACCCGTTAACAGCAATGTTTGTAATACCAACTCTTTCTGAGAAAATACCTGAAAGGGTTGCAAGTGTTAGAGCTGAAAACATAAAAATGGTTGTAGTTAAAATTAATATTCAACTATACATTATTCACCTCCTTTACTTGCTTGATTGCTTTTTTTCATATCTCTTTTTTTAGCTCAATTTTCTTTTACTTTGTCTCAATGAGATGTATAAATTAAATCTCTTTTAGTAATATATTGAATTTTTCTTTCGTGTTTTTCAGCTTTATATTGATTTTTTAATGTTTTAAGATTGTTTAAGCCAAAATCACTGTATTTTTTGAGATATTCGAATTTTTTCTTAGACATTTCGTCATAGATAGACATAATAGTATCATCACTTGCGCTTTTAAGTGAAGTTATTTTACAATCAACTCATTTTTGATATTCTTCTTTAATTGAAGCAAATTCTTTTTTGAGTTTTCTATTCCTTCTTTTTTCTTCATTAAGAGCTTTAACATATTCTCTATATTTAGCATTTTTGGTTTTCATGTATGAACCATATTCTTTTCAGTATTCTTTTTTGATTCAAAGCGTTCCGTATTTTCACATGAAACCAAGTGGTTTGAATTTATAAAGCATAATAGCTAAAGCAGCCATAAAGACAATTATACCAGTAATAATAGGGAAAAAGTCTTTTGTTAATTTATCTTTTATAGCAAATAAATCTCTTGTTGTATAAATCATTGAATAGAAAAATCCGGATAATGTTATACCAATAGGGTGGTTTAATGCAATAAGTGAAACAGCAATTGATTCAAAACCAATTGCAATAGGGTTGCTACCAAATGAATCGACAATTCAGCCTTCTTTGATAATAATGTAGTAGAATCCAGCGAAACCAGCTAAAGCTCCTGAAAATCCCATAACTATAATGGTTAACATTTTTTCGTTAAGACCAATATATTTAGCGTTTGAAGGATTTTTACCAACCATGTTTATTTTGTAACCTAATGTTGTGAAGTTGTAGATCACAAATAAAACAATAGCTGTTAAAACAAACATTCCAATACCAAAGTAAACAAATTGATATTTAACTGCATTTTCAATTGCAATTCTAGCACTACCTATAGTTGTAGGTGAATATGATTCTGGATTAAACATTTTCCCATTTAAACTAAATAATCATCCTGATATAAAGGCAACAATTCAGTTAATGAAAATAGTAGAAATAACTTCGTGAATTTTGCAATATGCTTTTAATAATCCAGCGATTGCACCTGTTGCAAATCCTACGATTATGAACAAAATAATTGCTAATCATAAGAATCCTGCGTCAACATTAAAGTTTCTAGCTTTAGCTATAAAACTGAATACTAAGATTGCTGGTAAAAGCATTTGACCAGAAATACCAATGTTAAATAATCCAACTTTAAAACCGATTGAAACAGCTAAACCAGTAAACCAAATATTACAAAAAATGTTGCAAAGTCAAATTTAATGTTTTTGTCAATAAAGCTATAATTTCATAATTTTATAACAAAGTCGAATGGTCCTGCACTACGTCCTTCCATGCTTTGATGAATCATGAAGAAGATGCTAGCAACAACTAATCCGAAAACAACAGCTCATAATGATGAGTATAATTTTCTTCTTCCTTTTTGTTGATCTTCAAGTGTAATAAATGATCTAAATTTATTTCAGCCTGTTCTTACTTTAGAAGAAAATGATAATTTTTCTTTTGTTTCTTTCATTATTGTGTGCCTCCTTCCGTTTGATGTTCTTTTTGAGCCATGTAAAGACCGATTTCTTTTCTAGTTAATTCTCTTGCGGGTTTTGAAAGTCTAATTTCGCCTTCGTTAATAACAGCAATAGTATCAGCTAGTGAAAGAACCTCGTCTAATTCATAAGAAATTAAAAGAATTGCTTTTCCTTCTGCTTTTTCTTTAAGAATTCTTTCATGGATATTTTTAATAGCCCCAACGTCAAGTACACGTGTTGGTTGAAGAATTAATATAAAGTCGTGTGGTGAGTTCATTTCACGACCAACAATGAATTTTTGTTGGTTACCGCCAGAAAGTGATCTAGAAGTTGAATAACCTTCTCTAGCACCACGAACGTCATATTTGTCAATAACTTCAGCTGTTTCTTTTTTAACAGCTTTAGGTTTTATGAAACCAAAAGTTTGATATTTTGGATCTCATAAACGTCTTAATATTGTATTATTTTTAATATTGTAATCTAATGCTAGACCATGTTTATGTCTATCACTTGGAATAAAACTTGAAGCAAGCATTGATTTTTCTAAGACTGAATAATTGTTTAAATTAATCCTGTCTTTAAGTTTCTCTTCATCTTTTTTGGTTTTAAAGAATTTAATAATTTTTACTATCAAGATACTTAAACCAACAGCAAATAGAGCAAAGAAACTAATGCCTATAGCTCACATTAATCAAATGTTTGAATAATTTTGCTGAAATGGACTAACACCAAAAAAAATTGATCCAAAAGCTATAAATAATGCAAGCAATGAACTTAAAATAATGAAATTCTTGATTTTATTTTTATGAATGAATTTAACTCTTTCCTCAACTAAAGGTGTTTTCTTTAAGTAGACATTACCTTTTGTAGGTTTAACAATTCCACTAACAACATATTCTAAATCTTGTTGTCCATTGCCTTCAACACCAGCAATAGCTAGAATTTCTCCAGCATGAACCTTAAGATTTAAGTTTTTAAGTGATTTTGTACCTTTTGTTGATACATTTTCAAGTTCTAAAACTGTGTCTTTATCTTCAAAAACGTCAAGTGATTGGTTTTGTGCCATAACAATATTGTCACCAACCATTCTGTTAGCCATTTCTTCAATAGAAGTATTAGCAACTTCGAAGTTACCAGTAACTTTACCGTGACGTATAACTGTTGCATAATCAGCTACAGCTTTAACTTCTTTTAGTTTGTGAGAGATAAAAATGATTGTTTTACCTTGCGATTTAAACAATTTGAATGTTTCAAGAAGACCTTGAATTTCTTCATCTGTTAAAACAGCAGTTGGTTCATCAAAGATTAGAATTTCTGAATCACGATATAACATCTTCATGATTTCAACTTTTTGCTGTGTTGAAACTGATTCAGTGCCTGTGTCTTTATTCAAATCAAAATTTAAATTAAATAATCTTTGAATAGTTTTAATTTTTTTAATTGATGGTTTATAATCCAAAGTTTTAAAAGTTTTTGATTCACTTTCAGCACCAAGAATTATATTTTGTAAGTTTGTGTATGCACCAACCAATTTAAAATGTTGGTGAACCATACCAATACCCAAATTATTAGCATCATTTGGATTATTTATTAAAACCTCTTTACCATTAACCTTGATAATTCCATGGTCGGGTTCATATAGTCCAAAAAGAATTGATAGCAAGGTACTTTTACCTGCACCATTTTCACCAATAAGTGCATGAATTGTACCTTTTTTAATTTGAATATTAATATCTTGGTTAGCTTTGATTACACCGAAACTTTTCGAAATATTAATAAATTCAATTGCGTTCATCTTTGTCCTTTTCTTAAATAAAATTTTTAGTTAATTTTGTTTCAGACTCATATTTATATATATTTTTATATATTAATATAAATAATAAAATTAACTATTTAAGTATATCAATTAATAGATAAATAAGAAATAATATGTTAAAAATATAGTTTATTATTTTTTCCAAAAGTGAGTGTGGTAAGTTTTAAGAAAAACATTTTTCTAAATCGATTGCTAAATTTTAAATTAAAAAGTCATTTTTTGACTGAAAGCGAAAAAAACGCAAAAGAAAATATTTTAATGCAAGCTAGCAAAATTCAGCGAAAGCTAGTAATTTTATATTTATTTTTTATTTTTAAATATTTGGATATAAATTGATAAATCATTGTTGGTTATATTGTTTGTTCATCATAACTTAAATTGCAAAATCATTTGCACTCATTGCATATGGATATTCTTTAATTTTATCTATTATGAATGAGTTAGTTTCATTTTGAATTCACGCAATATATTTATTTGATAAATTGTCTAATGTTTTTCCTTTTGGAATATATTTTCTAATAAATTTATTGTAGTTTTCAACACGACCTTTTTATGAAGAACAAAAAGCATCGCAATGATATATTTGGCCCACGGCATCGATTTCATCTAGCCTATAATTTTCAGGTCCATTATCAATAGTTAATGTATTGATTTTAAGGCTATTTTCTTATATGATTTCCTATAAAGTTATTTTGGTAGATAAAGAATTTTTTTATCTTTTTTTAAAGTGTACTTCTCTAGTCAATCTATTTATTAAAATCAAAATGAAATATTCATCCATTCTACACCCATGAACCGTATCCATCTCAAAGTCATAAATAGTTAAATAATTTGGTCTTTAACTTTTATGTTTTCCAACTCTTTTTTTCTTAGGCAGTTTTACAACCTTTTTTCTCTTATTTATCTTATTTGAAGACAAAAACGGAAAAACTGTTTTATCTAATTTCTTCAAAAATTAACAAAAAAATATACTTTTTTCATATTTAAAATAAAAAATGCTGGATATTTTCCAGCATTGCTACATTGTAATCTGAAACTTTACACATTTTAGATAGAATCTATAATGGGTGAAGTTTTTTTTATAAAAAAAGTCACCCAAAGAGTATTTGGCGACTGTCATGAAGAAATGCAAAAAAAATTATCATGAAATGAACGCACTGTTCTTGAATACTTACTTTGAGCAACTAATTATAGTATAACAAAAATAGCAAAAATATTAGGTTATTCAAGAACAACTATTAATAATGAAATAAAAAATAATTCTACTTTTTGGGGTTATTTTGCTTTAGATGCAATTGAAAAAACAATTACAAGAGAAAAGTGAAAAAATCATTTTAAATTTTTAAACAATTTAAATAAATATAAAGAATTTTCTTCTTTATTTAAAAAAGAATATGATGGCAAATTGTGAACTGTCAAATTAACAATTGAAAAAATAAAAAATCTTTATCCTAATATTGATGTTCCTTCTTTTAAAACAGTCTACAATTGAATAAAAAGTGGTTATTGAATTTTGAACTGAAAAGGTTTATTAAGAAAAGTCTACAAAAAAGGTGGAAAAAGAGAGAAACAAAGCGCTGCTAGAAGATTGGTTGGCGCACGTTATGTAAGACCTTTTTGATCTAGACCAGCAAACATAAACGAAAGAAAAGAATTCGGCCATTGGGAAATAGACCTTATTATAGGCAAGAGTAAAGGCACACATTGTCATTTACTTAGTTTTACCGAAAGAGTCTCTAGATATGGGTTTTTGGTAAAAATTCCTAATAAAAATCCATGAAATTTGAATTTGTATTTATGGAATCTTATTTGAAAACATAAATTAAATGTTAAATCTATAACTCAGGATAATGGATTAGAATTTTCAACATTATTTTTCATTGGATATAAACTAAAAATTTTTATATATAAAGCTGATCCATATGCTTCTTTTCAAAGGGGTTTAAATGAAAATTTCAATGGTTTAGTTAGAAGATTTTTTAAGAAAAAAACTAATTTTGATGATATTACTGAAGAAGAAATTCTAAAAGTACAAAATGAAATAAACAATCGTCCAAGAGAAATTTTTGGATATTTATCTGCTTTAGAAATGTACGAAAAATTGAATGCAGAAAATCCTTGAAATTCAACAGGTATTGACAAATTATTATATGGAAAACAAAAGAGAAACTGAGAAAGTAACAAGAATAGAAATTTGTTTTTCAAAAAAATAGGAAAATTAAAATAATATTTTAGAAAGGGCGACAAGTCGCCAAATATTTTAGGGAGGGCTAATGCCCTCACTTTTATTTGTCCTATTGGACAAAAAATAATAACAATATTAATAATTAAAAAGGAATATCGACATGCGATATTCCAAATTTCTAGGTGTAAACTTACAGATTACAATCTAGGAATGCTGGATATTTTCCAGCATTTTTTTGATTAATTATTTTGCTGCCTTGTTAATAGCAGAAATAATTGCTTCTAATCTTTCACTAACATTGTCAATTTTATTACCATCTTTTAAAGCTTTGTCACTATTAATATATTTAACGAAATCTTCTGGTAATTCTTTAAACATTTTAATTGCTTCTTTAATTTTGTTATTAATTTTTGCTTGTTCTTCTGTATTTGAGAAGTGGTTTTCTGCGACACCGATTCATTTTTCTTTTTGAGTTCCAAAGTGGCTTCATTTTTTGTCTGCTTTTTTGTCTTTAACTACATATGGTTTATATCCTTCTTCTTTTTCAAGAATAAGATCTAATAATGTTTCATAAACAGCTTGTTTAATGTGTTTTAGAACTGATGTAAGAATTCTGTCTTTGTCTTGAATCATACCTTGGTCTGAGTCAACACCAATTACATATTGACCTTTGTTTGCTAATCTTACAGTTTCAAATGTAGCAGGTCCAGCAACAGATAAGATAACATGTGGGTTGTATTTAACATCAGCTGGTGTTGAAGATAAAACATTATTAATAACAGTGTTCATTTTTTCACCAGCAGTAAAACCTGAGTCTAATTTAACAGGTGATGTGTGGTAAATTTTACTTGATTTATGTTTTTGGTTGTAGTATAGAATGCCTTTTGCAAAACCTTCGTTAAATGTTGTAACACCTGGGAATGCACCTACACCAAATGATGCAACAACTCTTTTACTTTCATCTTGTTCACTTAATCAACTTGCAATTGCATAGCCTGTTGTAAATGCAGATTCTTTAATATTGAATTGTAATGAGTAGAATCACTTGTACTCTGTTTCAATATCAAAGTCGATACCAATGATTTTGATTTGATTTCTTTCAAGTTCTTCTCTGTGAGCATCAATGTATTGTTTAATAGATTGTTGGTGTTTGAAGCCATTAAGTACTCAAATTTTGTGTCCGGCTGAAAGTGCACTGTTGTAAGTACTTTCAAAGTTTGAGCTAGGTTCAACACTGTTAATTTCAATACCAGTTTGTTTATTTATAGCTTTTAAAGCTTCAAAAGCTGATTGGTTAAATGATTTATCATCAATTTTACCTTCATCTGTAATAAGAACTGGTTTCAATTTTAACAATTCAGCGTTTTTAACAACTTGTTTTCCATTAGCATTTGTTGTGGTATATTTACTAATATCTTTCTCTTTGAATGAAATATTGGATTCATCGTTGTTTCCACAAGAAACAGCTACTGCAGGAAGAACAGCAGCAATAGGACTCAATCCTAATAAAATTTTTTTTGACTTTTTCATATAATCTCCTTATTTTTAATTGTATATTGAACATTATATAGATATTTTGCTTAAATATCTCAAAAATTGGTTTTTATTGGCAAATTTTGCAAAAAATAAGAAAAAATTGCAATTTGTTTTGTGATTTCATTTTTTAGTTTATATGTGCAAAATTATAATGTGGCAATATTAAATTTGTTATTAATAATATGAGATTAAAAAAATTTAAAATTTTTTACTAATTTTTATATTAAAAATGAAAATTGCGTCACATTTTTAACTGAATTAACAACTAAAAAGATAAATTATTGGTTATTTAAAAAAAGCGTCATAGAACATCATAAACGCATAAAACATAGATATTTATATTTAAGCTAAATAGGCTTTTATATTTTAAAAAATGTTATTAAAAATGCTTTTGCAAAACAAAGTTTATGTTTAACTTTTTATATATAAAAAAAATACTTTTATACTTATAAATAAGGCTAGTAAAAGTATTCTGTAAATTATTTTTTGAATTTTTTGCTTAAATTCTAAATTTATAACTTATGATTTTAAATTTGAGCCAATGTTACAATTACTATTGGTTCTTTTTCAAAAGTTTTGTAAATTCTTTTTCTAATAGCATGACGAGCTTCATTTTGGAAATCTTTTATTCCATTAAAACTTTCTGTTTCAATTAATTTTACAATTGTGCTTTTGATTATGTCGCTAGCTTCATTTTTTTCCTCTTTGTTTATGACACCAACAAAATTTATTTGTAATTTACCAGTTAATAATTTTGTTTTTGGATTATATTGAGAAGACACTAAAATCACACCTTCACGACCTAAAGCTTCACGCTCACTAATAACCTCAGTACTAATATCGCCAACACCAAAACCATCAATAATAGTGTCGCCAATTTCTTTAACTTTGCCCTTGGTTGTTGACATTTTGCCATCAATAAAGTGAACAATTTTTCCATTTTGCATAACTAAACAATGATTTTGTTTCACTTGTGTATTTTCACTTATAGCTATAGTAGCTTCATTTAAGTAACGATAAAGTCCTTGCACAGGAATCACATATTCAGGATTTAGAGCATTAACTAAATCAATTAAGTCTTGTTTAGCAGGACGACATCTATAAAATTCATTAGCATTAACATCACTAATTTTTGGAGTAATTCTTGCAATTTCATCTAAAGTTAAAGCTTCAAGACTTTCAAGTCCATTAATAGGAGGAGCAATCATTACAACAGTATCGCTTTTTTGAAGTTTTAAAAAAACATCATTATTGTCAGTTATTCTAATAAATCTTGAGTAAAGTCTTTCAGTAGCACCAGTTACTAAAATAACAGCATTTTTAACTTTGTTAGCATTACGATAATCAATTAATTCTGGTAACTTAAGTTCAGGATGAGCTTTTTTAATTAAATAAAATATTTGGCCATAAGTTTTACCATAAGTTACAACTGGACGACTTGTTTCTTGAGCTAATTCTAAAATTTTGTGGATAGCTACCATTTCTTCGTCATAAGCACCAACTATTATTCTTTCATTCTTTTTGGTTTCTAAAAAAACATCTCTAATACCTTCTGGTAAACCAATTTTTTCAATAGCCTTACCACCAAAGTTTGCTTTACCTGAGTCAACAACTAGAGCTAAAATTTTACGTTTTGTAAATCTTTGTTTAAGTTCATTAAAGCTTAATTTACCATAAATTCCTAAGTCACCTTCGACAAAGTTAAACATAAATAAGATATCTCCATCTGGTGTGATGAAGTCAAAACCAATGTGTCCTGGCATTGAACCAGCTAAATCGATTGGCTTAACAAAAAGTTTGTCACTAAATGGTGTAACTTTATTAATAACCATAACTTTGTAGTTTTTTGTTGGAATCTTATATTTTGATAAACGATCCATGATCATGATTTTGTTGAATGCTGAAGTATAAATTGTTAAGTTAGGAATTTTCATTAATAATCAAGGCAATGCACTAAACGATTCATTTTTAACATCAGAAATAAAGATACCTTTAATTCTATCTTTGTGTTTTTCTAAGTATTCAAAAGAAGGAATTAAAGTATCAACTCCATTATTTGAGTTGATTGGAATTTTAGTTCCTGAGTTAATAATGTAAATGTCATCATTGAATTCGAAAACATAACAGTTTTTACCATTTTCATCAAGCCCACCAAGAGCAAAAATATTAATATGATTCATATTATCTCCTTTTATAATAATCATTTAATGTGAAAAATAATAAACAATTAAGTTGACTTTAATTATATAAGAATAATTTTATTTTAAGTGAGCATATTTATTTATTTTCTCTTTTTTAATTTCTTTTAGTTTGATAACTAAAGTTATCTCACCTTTAAAAGGACTAGTTTTGTTTTTTGCTAATAGTTCAGTTGCGGTTGCATAAATAAATTCTTCATGTATTTTAGTAAGCTCTCTAGCCATAAATAATTGAGCTTGATTGCCTCACACTGAGTCAATGTCTTTTAAGAAACTTTCTAATTTATGCGGAGCTACATAAAAAACATATGCATGTTCACTTGTGAAGCTTTTGACTTGTTCTAATCTTTGGCCACTTTTTTCTTTAGGAAAAGTCATAAAAATAAAGGTATTACTTAGTCCACTAAAAGCAAAAGCACTAATAGCTGCATTGACTCCTGGAATTAGTTCTAATTCAATATTTTTTTCTCTTGCTTGTTTAATTAATTCAAAACCCGGATCACTAACTAAAGGCATACCTGCATCACTAATTAAAGCCATTTTTAAGTCATCTTTTTCAATTAAATCAATAAGGCCTTGGGCACTTGCTTTTTCATTGATTTTGTTGTATGAAATTAAACGTTTTTTAATGTCATAATGGTTCAATAATTTAGCACTAACACGAGTATCTTCGCAAGCAATAACATCAACAGCCTTAAGAGTTTCAAGAGCTCTTAAAGTTATATCTTTTAAGTTTCCAATAGGTGTTCCAACAATATATATTTTAGGCATAAGACTCCACTAATTTAATTAACATTTTTTCAGCTTGTAAGAAGAAATTTGCATTTGTATTTTGAATCTTTAAAAAGTCGTCTATTACTATAAAACAGTTGAATAAGTCAAATTTTATGTTTTTTAATTTAACTAATAATTTAGCATAATTTTTTCTACAAAAAGCATCAGCCGTTCATACTCAAGAGAAAACAAAACGCAAAGCCATAGCCAAAAATACAAAGGTATCTTTGCTATCTTTTTTTGTAAATCTAGATAAGAAAATATATAAATAATGAGGATTTTTAATACTTTTTTCTATAGCTTCAAGCAAGTCTTTAATTAGTTTAAATGATTCATTATTTGTATATTTTTTTACTTGTGTTAAATCAGCAAAAATATGGCTATAAAATCATATAGCTTCTTCTTTTAAATTTTCACCTTTTAATTCTTCTGCTAACTCTTCGTTTGAAGGCGCATTAATGTTAATTGTTATTGATCTTGATTTGATGGTTTCTAAAACTTTTGTTATGTTATTAGTTGTTAAAATAAAGACTACATTATCACTTGGTTCTTCAATTATTTTAAGTAATGAATTAAGAGCAGCTTTAGAAGCATTTTCAACATTTTTAAATACAGTTATCTTCTTTTGCCCTTCAACAAATGAAGATAAACTTGATTCTTCAAAGCCGGCCACAATTTTATCTTTTTTAAGATTATTTTCAGTGCCATTATCTTCAAAAATTTGAACATTAGGCAGCATTGTTTCAAGATCTAAAGATTGCAATTGTGAGTTGCAAAGTTTATTAATCATGTATAAAATTGAATCATCGATGTTTAAACCTGAAGGAGCTTTTAGTAAATAACAGTGACTAAGTTTGTTATTTTTTATTGAGTTTTCTAAAATAGTTATGACATTTTTTGAAACCATTATTTAAACTCCTTAATTCATTTTTTAAATCTTGGATGTTTATCTAATTTTTCAAATGATTCTTTTAAAACTTCTTCAATACTTTTTGTAGCATCAACAACAATGAATCTATCTTTTTCTTTTTTAATTAGCTCATGATAACCTTTGTAAACTTTTTGGTGGAATAATTCGGTTTCACTGTCTAATCTGTCACTAACAATTCTTATTTGTTCTCTTCTGATTTTACTTTCTTCAGGTTTGATTTCAAAAAAGATTGTAATATCAGGCATAGTTTTATCAATTATTAAATTAGTAAGTTTTTTAACAAAAGTAATACCTAAATTACGAGCATAACCTTGATATGCAAAAAAGCTGTCGACATAGCGATCGCACAAAACAAGTCTGTTTTCTTTTAGAGCGGGTCATACTACTCTTTCAAGATGAATTCGACGACTAGTTGTATATAAAAGAGCTTCAGAAACTGAAGAAAGCTTTGAATCTTTATCCAAAATTAATGATCTAATTTTTTCTGCTTCAATTATGTCTTTGCCACCCGGTTCACGAGTCAAAACATAATTTAATGCAAGCTTTGGATATTTTTCAATTAATTCAATAATAAGGCGGTTAATTATTGTAGTTTTACCACTACCATCTGGTCCTTCAAAAGTAATAAACATAATTAACCTCCCTTAAGTTTTATTTGTTTCTATTTTCAATAGATTGTTTGATTGTGAAAGTATCAAGATATTCTAAATTTGAACCCATAGGCATGCCAATAGCTGCTCTTGTTACTTTAATTTTAAATTGGCTATTAATCAATTTTAATAAATGACCTGTTGTCATTTCGCCTTCTAAAGAAGGGCTCAAAACTATGATTACTTCATCAAATTTTTTATGTTGAATATAGTTTATTAATTCTTTGTAATCATAGTTTTTAATTTCCTTATTATTCTTTAAATTAAACAAATAAGGCAAAACATAGTAGTAGCCATTGTAAAAATTCATGTCATCAATTTTTCTTAAAGTTGCAGCATTTTCAACTATCATTAAAAAGTTGTATCTGCTCTTATCATCACATACTAAACATTTGCCACCTTCTTTTAAAAAGTTACAAATAGAACAATATGAAATGTTGGTTTTAAGATCATTAATTGACTTAACTAAATTGTCAATATATTCTTGATCTTGTTTTAAGAAAAAGTTTGACATTTTGTCTGCTTGCTTTTTACTAATTCCTGGAATTGTTGTTAAAACTTGATTAAGTTTTTCAATTGTTTCAATTTTCATTATTAGAAGCCAAGTCCACCTAAACCAGGCATAGCTGGCATTAATTTTTCTTGTTCTTCATCAATTGTGTCAAATAACTCATTAATCACAAGTGATAACAAGTCTTCTAAAGTTTCAGCATCATCTGGGTCTAAAAGAATAGAATCTTTGATTTTTACTGAAACTATTTTTTTGCTTCCTTTAGCTGTAACTTCAATTCCATGTTTTTCTAATTTAAATGTTTCTTCCATGAATTGTTCAGTTTTTTCTTGAACTTCTTGTTGCAATTTTTGCATCTTTCTTAACATTGCTTGATCCATAATTTCTCCTTAAAAGTGTATAAATAAAATTTAGTATAAACTAAAAATTATTTTTTGAATTTATCAAATAATTTGCCTGTTGGACTCTTTACATTTTGTAAAACAACTGGTCCTGGTACTGTAAAGCTTGTGTCAAATTTTGCATCTTTCATTTTAGCTGCAAAATCTTTTGCTGTTTCTTTGTAATCATCACTCATAATGCAGTATAAGTGTTTGTAATCTTCAAAGTAATCTTGAATAAATTTTTGAATATTTGAGTTGTAACCATTTTCTTGTAAATAAACTAATTCTGGTACATTTTTTGAGATAAAAATCATGTAATTAGGGCCTGCTGCGGCAATTTTACATTTTTTAAGTACTGTAATTAAATCACTGTAAATTGGATTTTGAACTGTGTTAATCACAATATCTAAATTTGATTTGTATCTATTTAAAAATGTTAATGTTGATTGTTTAAGTAAGTTGATTAAATCTTTTTTACTTAAGTTTGATTTGAATGTTTTCAAGTTTTTGTATTTTGAGTCAAAAGCAGGGATTACTACTGGTTTTGGCATATTTTGAATTTCATCATCTAGTTGAATTTCTGCTGTACTTACTAAACCATCATCATAATCTTCTTCTATTTCAAAATTGCTACTGAGAAGATCTTGTTCTAGGCTAAAATTGTCATCAGCGTCACGATTAGCTTCAAGTACTAATTCTTGAGTTTGATTTAAAACTGTTTTGATTGTGTCATCTTTTTTAGGTTTCTTTTTGTTTACATCAATTGACATAGTTTGCTCCTTACTATTTTGTAATTCATTAGTTTGATTAAGTTTAATTAAATAAAGTTCGATTAATTGGAATGGGTTTTCTGAAAAAATTAATTCTTTGAATAATTTATAAAGTTTTTCAGATGAATTGAATGCAAAATTAACATCAATTTTCAAAGTATTTAATTCTTCAAGAGATAAAAGTTCTAATAAATTAGCATCATTTGATTTTGCATAAATCAAGTAATCCTTGAACAATTCTATTAAGCTTTGAACAAATTGATTTGAATCAATTCCCGCATTTTTAAGGTTATTAAATAAAATTAAGACTTCCTTGATTTTGCCTTGATAAAGGTAGTTTACAATTTGAATTAAGCTTTCATTTGAAGTAATTCCAAAAGCATACATAACATCTTTGAGCGATATTTTGTCGTTACCATAAGCTGCAGCTTGGTCGACTATTGAAAGTGCATCACGCATACCACCAGTTGCTAATCTTGCAATATAAGCTATGGTTGAATCTTCATATTTAATTTTTTCTTTATCTAAGACCTCTTTAATTTGGTTAATTAGAACTTGGTTTGATATTTTCCTAAAATTATATCTTTGGACTCTTGAAAGAATTGTTAGAGGTATTTTTTGAGGATCAGTTGTTGCTAAAATAAATATTGCATGTGCTGGTGGTTCTTCTAATGTTTTTAAAAGTGCATTAAAAGCTCCTTTTGAAAGCATGTGAACTTCATCGATTATATAAACTTTGTAGTTTCCACTTGCTGGTGAATGTTGGATTTTATCTCTTAAATCTCTAATTTCATTAACACCATTATTTGAAGCAGCATCCATTTCGATAACATCAAAATTTTGATCCGCTTTTTCAATACAACTTTCACAAAGTTTAGTTAAATCTTCACCATGACCACAGTTAACTACATTAGCAAAAATTTTAGCAACTGAAGTTTTACCAACACCTCTTGGTCCACTAAATAAGTATGCATGACTTATCTTGTGATTTAAAATGATATTTTTTAATGTTTGCACAATATGATCTTGACCCTTAACATCATCAAAAGACTTCGGACGATATTTTCGATAAAGTGCTTTATATGCCATAATACCCCTTTCCAAATAATTAATATTAACATTAAATTACTTATATATTTATAATCATATATTAAAAATTAAAAGCCTTAAAAATAAACGCAAAAAAGTTTTCTAAAATGTAAAAACCATGCCACACTATAAAAATTTTTTTTATAATTAATATGCTTTATAACTTTAAGGAGGTTTTTATGTCTGAAATAATAAAAGTTGAAAATTTAAAAAAATCATATGGCAAAGTTGAAGCTGTCAAAGGTATTTCTTTCATTGTAAAAGAAGGAGAGCTTTTTGCATTTTTAGGGCCTAATGGTGCAGGCAAAAGTACCACAATTGATACCTTGTGTACATTGAATGATTATAATGAAGGCCAAATAACAATTTGTGGTTTTGATTTAAAGAAACAAAGTGATGAAATCAAAAAACAAATTGGTGTGGTTTTTCAAGATAGTGTATTAGATCGAAAATTAAGTGTTAGAGATAATTTAACAATTAGAGCAGCATATTATTATCGTAAAAAAGCAGATATAAAAGCAGCTTTTGAAAGGGTTGTAAAAGATACTCAAATTGAAGATATTTTGAAAAGAAAATATGAAAAACTTTCAGGTGGTCAAAGACGTCGTGTGGATATAGCTAGAGCCTTATTAAATTATCCAAAAATTTTATTTTTAGATGAACCAACAACAGGATTAGATCCTCAAACTAGAAAGCTTATTTGAGAATTAATTTATGAATTAAAAAATAAAAATAAAACAACAATATTTTTAACTACTCACTATATGGAAGAAGCTGAAAAAGCTGACTATGTTGTGATTATGGATAATGGAAAAATTGTGGCTAAGAATACTCCTGCAAAACTAAAAGAAGAATATACAAAAGATAGGTTAAAAGTATATCTTGAAAATGATGTAAAGTACTTTAAAACAGTTGAATTGTTAAAAAACAAAGGTTTAAAATTCAAAGAATTTAAAGATTATATTGATATATATTTAGACTCTTCGTTTAAAGCTATAGATTTGGCAAATCAACTTAAAGATTTGATATCTTCTTTCGAAGTTTTAAAGGGTGATATGGATGATGTATTTATCAATATAACTGGAAAGGAGATTAGATAATATGGGTGCAATAATTAAAAGAAATATTAAAAAATATTTCATGGACAAAACTAATGTTTTCTTTTCTTTATTAAGTGTTATTTTAGTCTTCTTTATAGTTGCAGTTTTTTTAGGTAAATCAATTAGTGATGGACTTAAAGGATGAGTGATTGAAGATAAAAGCAAAAGCGCTAGAGAAATAATGAATTTATGAATGATTTCAACTACAACGACAATTGGTTGTATTACAGTTAGTTTAGGTGCTTCAAGTTTCATGGTTGAAGATATAGAATCAAAAGCCTATAAAGACATTTATGCTTCGCCAGTTAATAAATCTTCAATAATTATTGGAAACGCTATTTCTATTTATGCTGTTACTGTAATTATGTCAACTGTATCATTTATTATTAGTTTTGTGTATGCATTATGCTTTGGAGCAAAATTCTTATCATTTTTACAAATTGTTGAAGTAATTGGATTGATTTTAGTTTCAAGTTTAATAGGAAGTAGTTTTAGCTTTTTAATTGTTTACTTCATTAAAAAACATAATGTTTTTGCAGCTATCAATGCTTTAATTGGATCTATAGCAGGTTTTATAATTGGTGGTTATTCAACAATGGAATCACTGCCTGATGCGCTTCAATGAATTATTAAATGCTTTCCTGCTTCGCATGCTAATGTTTTATTTAGACAAACCTTAGTTTCACCTGCTATTGATCGTCTAATTAGTGATCCAACACACATAGAACAATTTAAAAAAATAATGGGTTGTTTTTACAAATTTAACGATTATCAAATGCAATGATATGTTAATTTCATAATTGTAGCAGGCACTTCACTATTGCTATATTCAATAGCTTTTGGAGTTCAATTCACTAAAAAAATCAAAAATTAAAAACCGCGCTATGCGGCTTTTTTATAGTTTTTTTAGTAAAAATCATTATAGATTTTTTAATGCTTCCAAAGCTATTTCGAACATTTCTGTAAATCCTGTTTCTCTAGTTTTATAATCACTAAATTCCATTTTAGCTAAAGAGTCACTAACTTGTAAAATGGTTGCGGCTTTTTTATTGCTTCTTTTAGCTTCTGCAAAAAGCGCATAACTTTCTGCTTCAACTACTTGACATTTTGATACTTTCTTAGTTTCCTCTAAAGGTCTAGTTGCATAGAAAACATCTGTTGAGTGTACTGAAACTACTGGTGCTTTTTTGTTTAATTTTTTAGCTGCCTTAATTAATTCATCTGTTACACCTTTGTCTGGAAAGTATTCATATGTATTTTCTTTATTTATTAATTGAGCTATTGAAATGTTGTCGCTAAAAGCTCTATTAATAATTACTGTATCCATAACATTGTGTTCGTCATAATATGAACCAGTTGATCCTAAACGAATAATAACATCTACTCCATATTCATTATAAAGTTCATGAGCATAAGCTCCAATTGAGAACATACCCATTCCATGTCCTCCAAAAGTAACTTTGATACCTCTATATTCTCCTGTGTAGTAATTTTCATTTCTAACTGAGCTTACTTCTTTTACATTTTTTAAATATTTATGAGCCATAAATTTTGATCTTAAAGGGTCTCCTGAAAGAAAACAAATTCTTGCTATTTCACCTTTTTTTGCGCTGATGTGTGTTGACATAATAACTCCTTATTTTGTTTATATATGATTATTTTAAAACAAGAATTTTAACTTTTTCTTAAAAAATATGCTTATTTTTTTATAAGGTGAAATTAATTACTTAAAGAGCCAAAAAATGAGTGAAATTTTAAAAAGCTTTAAAAGAATGATTTATTCAAAAAGTGCAAAAAAAACTTATTTTTTAAACAAAATTTAAACTTTTTGCAAAAATACATTATAATTTAGTTAACTAATAATTAGTTAGTTAAATGATTTTTAGAAAAAATGGAGGAAATCATGAAAAATAAAAAAAGAAAATTATTCTTAATCCTATCTTCATTAAGTACATTACCTTTTGCTTTTGTAGCAGGTAGTTGTGTTGGTAAAGATAATGGTGGAACAACCGGTAAACCAACTAGTGAAGAAACAAAAGCTGATTTAAATAAACTTGCAAAAAATATTGTTTTTGATGTTAAAAATAAACAAGATAAATTTGCAAAGGATATTAAAAACATATCAAATTTAGTTGTATCAAATTTAGATTTAAATAAATATGAATTAATACATTGAAGAATTGAAGCGCAAGAAACTTTAATAAAAATTTACTTTAAAATTAAGAATTCTGCAAATCTTGTAAGTGACGAAAATACCTATGAAATAACTGGATTCAAAAAATCTGCTACTACTTCAAACCCAGGGAAAGTTCCAGCTAATAATCCTTCAGAAAGTTTACTTAAAGAAGTAAAAAAAGTTAAAATTGATTATTATAATCCAGCTCTTAAAAAAGATGTTTTAGTAGATAAGACTGAAGCTAGTGATTTTGTTGCTACAGGTTTTAATGAGGACTTATTTGAAATAAATATCAACTACGACAACAAACTAGTGGATACTGCAACAGGCGATTTATATATATCGTTTAAATTAAGAGAAAAAGCAAATAATATTTCTGTTGACGGTTATTGTAAAGTTCAAGGTTTCAAACCATTTGATAGTGTTAAAAATCCTGATTTAATAGATGTAGCTAATGCAGCAAAAGCTGTAAATGTTGCGTATGAAGATAAAGGTAGCTTTTCTATTTATGAATATAATGCGAATTTAGTTAAATTTGAAAATTATGATTCATCTATTTATGATTTAAGCCTAGAATCTTGAACTTTAAATATTTATAATGTTTCAACTGTGATTATTAGTTGAAGTTTTTGAAATAAAAATACAAATAGTTTAATAAAAAAAGTTACTGTTATTGATGGTTTTAAACCTGCTTTATACAATAAAAAATCTCAAAATTTATCTGTTACACCAAGCGGAGTATTTGCAACAATGCCCACTACACAAAAAAGACCAACATTTAGAATATCTAAAGAATTAGATGCAATTTTTGACAAAGCAAAAGAGTTTTATCTAAAACCAGAAAACTTCGATATTTACACAGGAGAATTTAAAACACAAGTTTATGGAAAATATAGTTCTTGACTCATTAATGATCTTACAAAATCAGCACTAGAATCATTAGCAAAAAAAGATTTATCTAATCCAATTACAACACTTAATAGTTGAAACATATTGGAACGAGCTTTTATATATAATAAAACTAAATTTGTTGTAACTGATTACACAATTAATGGTGATAAAGTAGAATTAAAATTTAAACTTTGTCGTAATTTAGATGATAAAAGTAAAAAGTCATTTTATGAAATATCAACAAAAGAATTCACAAAAGAAATTACTTTAGCTACAGAAAAAACAACAGAAAAAGCATATTTGGACAAACTAATTGATAATTATGACTTTTCACTTCCAGAAGGCTTACAACAATATCATGCCCATAAAGTTCTTTCTCAATTAAAACAAAAACAAATAGGATTTTTTGAACAAAAAAAATGAAAAAATGCAATTGCTGAATTAAAAAAAGAATGACCAGATTTAAAAAGTGAAAACGTTTATTTAAATATTATTGATTATCGTCAAAAAGATGTTAATTCATTTGAAGCGGATGTAACATTTAGTTATCAAAAAGATGGAAGCAATAAAAATCAAAGAGTTATAAGTAAAGTTAGAACCTTGCAATTTAATAACTTTAAAGATTTAAATCCACTTATTAAATTAGTAGACAGATTGAATAAGTCAATGGTAGCTTATATTAATCCAAATGAAAGTCTTGATACATTTAATGAATCATTAGGACGTCAATTAAAATCTTGAATGAATGGTAGTAATCTTTTTTATACAGTATCAAATAAATTTAAAGCAATAGATTTTACAGATCAAAGTGCTTTAAATGAAATTCAAAGTTATTATGATAATGAATGAGATATTAATGCTTATGATTCTGGTGGAAGTTTAGGCAATTATGCTAAATTAGCTCCTAATTGTAAATTTGAAACATATTATGATTCATCAAATAATTTTAATTTAAATACAAGAATAATTTTTGAAGATTTAGAATTATATGAACCAGACTATAATCATTTTGACTTGGAAGCAATTAAATTAACACATAATTCAATTAATTCGCCAAAAACCTTTGATCCAAAAATTAATAATAGTTTTCCATTCACAGATTCGAAACTTGAAGAATATGTTAAAACAGACGATTTTAAACACAATTTAGAAGCAAATATTAAAGAAAACTTTTATTTACTTGATTTCATTAAAAAAGAAACTTCTGATAATAAAAAAGATATAAGCAAATTTGTACTTTCTGATTTAGAACTACAAAAAGAATATGATTCAATATTGTTAGATGAAGAATCAAAAAAGATCATTGTTACAAATATTGAAAATTATTTAAATAATAAATTTGATTCTAAATTTAATAAATTTTTAAATTCATATTTCAAACAAGCAGAAATAGCTAGAAAGAACAAAGCGATTAAAGATGATCAAAAATTGTTTAGATATACAATTAAAGAATTCTCAAATATTGATGCCTATCAATCTAAAATTAAAATTCAAGTTGAAATATTCAAAATAGAAGCTAAGGCTTGAAAAGTTGTAGATGAATTTGAATTTGTTTATCAACATGATTCAGAAATTAGAGAAAATCATTGTCCAAGTTTAAGCACAAAATCAACTGAATTAAAAGATGCAACAATTGGTATTTCACAAGAAATTCAAACACAATTTATTAACTTAATGAGTGCATATGGTGTTGAACAAGCTATGTCATCTAAAGCAGACAAACTAGAATTCCTTGAAAAATTAATGTCTGTTGGTGGTGCTAAAATAAAAGAACATGATTCAGCTATTTCCTTTATTAATGCTTATTTATTCAAGATGTTAATTTTCGAAAATGTTAAAGTTTATGAAAGACTTAATCGATTTGACAAAGTAGAATTTACATATGATCAAGTAAATAACAAAGTTGTTGTTAAATTGGTATTTGGATATGAAACACATGTATTTAATATTGATAGCAATAAATTTGACAAGAAAGTTTTAAATGTAATTAAAGTTTTTGCAAACAATGAGTTAAATAAATTTATTGATGAAATTATAAAAAATAATTGAATTAAATTAAAAGATAATAAGTATTATGATGAAAATTTACATGATAGAGATCTTGAAAAAGTATTTGAAATTGATTATACAAAAACGACAGATAAAGGCAACAGATTTAAAGTTAGAATTATTAGTCATAGTTTAAATAATCTTCAAATATCAGTAATAGATACCAAAATGAGAGAAATGTATGGATATAGTTTACCTTATGTAACCGATGGAATTAATTTAGATTTTGATATAGATAAGTTTCTAAGCCCAGTAGCTTCACAACTTAAAGGTATTTGAGCTGAAATTGAAAATTTTACAGTTGCTCAACAAAAAGAATTATTTGCATTAATTAAAGATAAAACAGATTTTTATTCAATTGATTCTCATGAAAAATTAAATGAATTAATAGTTAAAATAAAATCATTTATAAAATTTAAAAATGTTGAAATTGAAAGTAATCCTCATCTTAAACAACAAGTGCTTGATAATTTAGTTTTAATTGATGCAGTAAGAAAAACGTATGATGAATATGAAGTTATTTTCCGTATAAAAGGAAAAAATCAAAAATTAGTAATATCATTCCAAAATAAATAATAATCAAAAAAGTCTAACCATTTGGTTAGATTTTTTGTATAAAAAAAGCAACAAATGTTGCAAAAAGGTCATTCTGATATGGCAGGAGTAGCAGGACTCGAACCCACAACACACGGGGTTGAAGCCCGTTGTTCTACCATTGAACTATACTCCTATTAATAAGCTAAAAATAATTATACACAAAAATTAAATTTTCTTAGCATTTTCTATTGCATCAATAATATCATTAACATTTTTAATTTTTAAAAGCACTTCATCTTCAATTCTAATTTTGAATTCTATTTCACTTTCGTAAATTAATTCAGCAAGTGATAATGAATCAATTTTTAATTCTTCGAGTGTTGAAGTAAAAGTTACCTTTTTCTTTGAAAGTTTGTCTAATCTTGCAATAACAATTTCTTTAATTTTATTTTTTTCCATGTTCTTATTATAAATTAAAAAAGCTAAATATTTTGAATAAATACAAAGCGATATTTACCATTGATATCTTTTTTAATTTGTGCTCCCTTAATTTGTTTTCATTTTTCAATGTGTTGAGGATTAATTTCCATAATTAATCAACCGTTTTTCTTTAAATAATTCTTGTATTTTTTTAAGATTTTATCATAAAAATATCAGCCATCTTCAGGTGCACATAATGCAATTTTAGGTTCAAAACTTAATGCTTTTCAGTCTAAACTTTTGTCATTGCAATTTAAATATGGTGGATTTGAAATGATAACATCAAATCTTCCCTTAACATTAGTAAATAAGTCACTTTGGTAAATTTTTAAGTCATGATTAAAACCAAAATTTACTATAGAATTTTCAATGGTTTGTTTAATAGCTTCATCACTAATATCTACTAAAGTTACTTTAGCTGAACGTAAGTTTTTCTTAATTGCTAAACCTATAAAACCTGAACCACAACAAAGATCTAAAACTTCTTGATTTTCAGCTGCAAATTCTTTTAGATAAATATCAACCATTTCTTCAGTTTCATATCTTGGAATTAAAACTTGTTCTCTTAAATTAATTCTAGTATTTAAGTATTCAACATAGCCCATTATTAATTGAATTGGATAATTTTGCCTTAATAATTCATATTCTTTTTCAGTAATTTTAGGTTCCAAACCATACCGCTTTTTTTCTAAAATTAATGTTTCTTCGTTATTCATTTTATAAGCCACTTTCCTTTATTTTTTGATTTTGTTCTTCTGTTAGTAATGCTTCAATAATAGTTAATAATTTACCTTCCATTACTTGTTTTAAACTTGTTGAATATCCAATTCTATGGTCTGTAACTCTATCTTGTGGATAGTTATATGTTCTAATTTTTTCGCTCCGATCACCATGACCTGCTAGTTTTCTATACCCTGCTTCTTCTTGTTGTTTCTTTTGAATTTCTAAATCATAAAGCTTACTTTTAAGAACTGTTAAAGCAGTTTCTCTATTTGCTATTTGACTTCTTTCATCTTGCGATGAAACAACTATACCAGTAGGCAAGTGTGTAATTCTAACCGCTGAGTCTGTTGTATTAACTGATTGGCCACCAGCTCCAGATGATCTATATGTATCAATTTGTAAATCTTGTGGTTTGATTTCAATATCAACACTATCATCAATTTCAGGCATAACAGTAACTGTTGCTGTTGAAGTATGAATTCTACCTGCACTTTCAGTTACAGGTACGCGTTGCACACGATGCACTCCACTTTCAAACTTAAGTTTTGAATAAGCTTTATCACCCTTAATACTAAATACTATTTGAGTAAAACCACCAACACTAGCTGCTGAAGAAGATATAACTTTTAATTTGAAATTTAAATCTTCTGCAAATTTTGTGTACATTTTGAATAAGTCGCCAGCAAAAATATTAGCTTCGTCACCGCCTGCTGCACCTCTAATTTCCATAATAACATTCTTGTCATCGTTTTCATCTTTAGGCAGAATTAAAATTTTTAATTCTTCTTCAAGTTGTGCTAATTTAACTTCATTTTCTTCAATGATACTTTTAGCAAAAAGAACTTCCTCTTCATTTTTTGAATTGATCATTACTTTTGCATCATTGTAGTCATTTTGTAAATTTAAGTACTTAGTAAAGGTTTCTGCTATATCCTTAATTTTGTTGACTTCTTTACTTATTTTTGTGTATTCCTTGATGTTATTGATAACTTCATGATCATCCAATTTTTTAACTAAATTGTCATAGGTATTTTTAATATCAGACAACGAATTGAACATATTTTTTTCCATGTTTATAATTATATAAAAATATTCATTTTTAAAAAGCATATTTATTTATAGCTAATAATCAGTCTAAAAATAAAGCTAAAAGCCTTTTTTATAGGTTTATAAATAAAATTTAAACTTTTAAAAATGATATTTTCAACAATAATTTACCATAAGATTCATTTTAATTAATTTTAGTTTAAAATATAAATATAAAAGTCATTTTAAGGAGAAATTTATATAATGGATAAAAAAAATGTAATGCTTTTTGGTATGCCGAATAGCTTGAAATTAGCAGAAAAAATTTCAAAAATTATTAAAGTTCCTTTGACAGGAATTACTAAAACAACTTTTGCTGATGGCGAAGTTATGTTAGTTAGCGACAAAACAGTTAGAGAAAAAGATGTTTTCGTTATTGCTTCAACATCAAGACCAGTTAATGAAAACTTAATGGAGTTATTCTTATTTATTGACTCGCTTAAAAGAGCAAGTGTTAAAAGTGTTACAGTTGCTTTAAGTTACTATGGATATGCACGTCAAGATCACAAAGCAAGTGGACGTCAACCTATTGGTGCAAAACTAGTAGCAGACTTATTACAAACAGCGGGTGCTGACAAAGTTATTGCAGTTGATTTACATAACCCTGCAATTCAAGGTTTCTTCAATATTCCTATTGATGATTTGCGTGGTGCTTATACTTTAGCAAAAGCTATTAAAAATACACATGAATCATTTACTGTTGTTTCACCTGACCACGGTGGAACAATTAGAGCAAGAAAACTTGCTGAATTAATTTCAAACGATGTAAAAATTTGTATTATTGACAAACGTCGTATTGGCACAAACCAAACTGAAGTTTTAGGACTTATTGGTGATATTGAAGGTCAAAATGCAGTTATCATTGATGATATCATTGATACTGGAGGAACAATTTTAAAAGCAGTTGATACATTAAAAGCACACGGTGCAAAGAAAATTATTGTAGCTGCAACTCACGGTATTTTTACAAAAGGATTTGAAATTTTTGAAAATAATCCAAATGTTTATAAAGTAATTATTACTGATTCAATTGAAAATGCAGAATTAAGAGATGGTAAATTCAAAAAACTCGAAGTAATAAGTCTTGACAGATTCCTAGCTAATGTTATTGAATCATCAATTAGTGGTAAATCTATTTCTCATTTATATCAACAATTTGCAAAAGAAATTACTTCAAATAAATAATGAGCTATAGAAAAATTACTAAAAAAGATTTACTTGAAAAATATTCTTCAAAAATTAGCATATTAAAAGAATATGCTCAAATGATTTATGATCAAAATCAAGTTATGAATATCACAGGTTTTAAAACACTAGATGATATTTTTGAACAAGGTATACTTGATTCTATTTTAGCTTTTGAAAATTACATGATGCATTATGATGAAAATCTAGAAAACAAAAAAATATTAGATGTAGGTGCCGGTGCAGGATTTCCTTCAATTCCAATTCTAATTGCATTAGATAATAAGTTCAATTTAACTATTATTGAATCTCAAAAGAAAAGATGCAATTTTTTAGAAAAAGTTAAAGCACGATTTAATTTAAGTGTCAATATAATTAATTCAAGAGTTGAAGAGTTTTTTAAATTAACAAATTACTTTGAGATTATTACTGCTCGAGCTGTTGCAAGCATTAAAGTTTTATATTTAAGTGCAGCGCACTTATTAAAAAACAGAGGAAAATTTGTTTTATTAAAAGGCGAAAACTTTAGAGATGAGCTTACTGATATTGTAAATATTGCTAAATACATGGGCGAAATGGGCGGCTTTGAATATGATAATGTTTTAAATGAAACGTCTAGCGTGGTGGTTATTAACAAAGGAAAAATTCCTGATGATTGACCACATTCATGAACTAAAATTAACAAAATGTAAAGTTAAAATAAAGTCTATTAATTAGGCTTTTTTATTATGCTCTTTTGTATCAATTAAATTGTTTTATAGTTAAATAAGTCAAAAATAACTAGCTCTTTGCTAGTTAAAAAATTATTCATATTCTTGACTTTTAATTTCTTTGTATTCAAAATCAGCTGGAGTTTTTTTACCTAAATATTCGATTTCTACAGTTACTTTTTTATTTGCTTCTGAACATTCTAAAACTCTACCTTGTTCACCCTTGAAAGGTCCATCAATAATTTCAACAATTTCACCAATTTGGAATTTTGTATGTGTAACGCCTTTGTCAAAGTCTTCTTGTAATTTGATTTCTTTTTTAAAACTTTTATCAATTTGGTGTTGTGAAACTGGAGTAGGTTTTGCTCCACGGCCTGATGATCCAATTAAACCTGTTACATATTGAGTATTACGTACAATAAATCAAGCTCTATCTGTCATGTCCATATTAATAAAAATGTAGCCTGAATACATGTTTACATATTTAACTTTGTATTCTTCACCATTTTTTTGTTTTTCTAATTCTTTAGGTGTTAAGGTTGGTTTTTTGAAAATTTTAAAAGCGCCTTCAGGTGTTGCTTCATGATTAAAGCAATCTTCAACTTGTTCACTAATAATTCTATTTTTTAAAGCTTCAACAACCTTATCTTCTTTTCCAGAAACTGTACTAATCATATATCATTGAAAGTTCTTATCCATTATATCCTCCAATTATTTAACTCCTGCTTTAGTAAGAATAAGTGAGAAAAGAACGCCAAGTAAAACAGCACACAATGTAAATATCAATGTAAAAATAACTATTTGAGCAAAAGAAATTCAATTCTTTCTTACAGAAGGTCATCTAACTCTTTTCATTTCTTTTACAAAACGTCTGAATAGATACTTTTTAGGTTTTTTAGTTTTGACTTCTTTTTCCTTTTTCATTATTTTTCCTCTTTGTGTATTGTGTGCATTCTACAGTTTGGACAAAATTTTTTAATCTCTATTCTTTTAGGATTGCTTAAACTTTTATTTGTTGAATAGTTAAGTTTTTGGCAATTTTCACAAGAAAGAGTCACTTTTCTTTTTTGCATAATCTAAATTATAAAGAATAATTAAAAAAAAGCAAATTTTATTTTATAACTTATTTTTGCTTCTTTTATTAATATTTAATAAACATTTTTAATGGGCAAAATGAAAATAATTATTAAATTTAGTTAAAAATTTAGTTTTAAATTCTTTTAGTTTACTAGGTGTAATCAATTTGTCCTTTTGATTGATACTATTTTGCAATATTTTAATTATATTTTTATCTTTGTCAGTTAATAAATGATAAAAGTTTTCAGTATCAATTTGATTAATTCTTTGTTCAATTTGTTCTTCAGAATGCTCATCTAAAACATATAACAATTCATCAGTTGAAGTCATAAGTGAGTTATGAATTCTTTTTTTTCTAAGCCAATATCTAATTTTATTTAATGTGTAAAATTTGGAGATATTTAAAAAGAAATTTAAAAATTTAACATTTTTGTCAGTTGAATTATACTTTTTAACTTCTTCTAAATAGGTTTGCAAAAATTCAATATAAATATCTTCAGCTTCTAATGGAATATAAGGAAATCTAATTAAAGTGCTTTTGATATTTTTGATAAATTCATTTTTGTAAAAATAATCCAATATCATAATCAATTGATTGTCTTTTAAGCTATGTAAAAATTCATCAAACTATTGCGTGGGTGTTTGATGTAAGTCTAAAAATTTTTGTAAATATTTCATGTTAGTCCTTTGTTAATTCATATAACAAAATACCAGTTGCAACTGAAACATTCATTGATTGAACACTTCCTTTTTGAGGAATGTAAATCACTTGGTCAGCTTCATTTAGAGTTGATTTGCTAACACCATCTCCTTCATTGCCAACTACTAAAACACTTGGTTTATTAAAAGTAACTTGATTTACTTTTTGAGCAGTTTCATCTAAAGCTGAAACATAAATTCAATAGTTTCATTTTTTAAGTTTTGTTAAGGTAGCACTAATACTATTAACTTTAATTATTTTCATATTAACAAAACCACCTGAGCTAACTTTTAAAACTGTTGAAGTAATATTAGCTGATCTTTCTTTACTAATAATAAGATGCTTAATTCCGCAAGTATTAGCAGTTCTAATGATTGCACCTAAATTATGAGGGTCTTGAATGTGGTCTAAAACTAAAACATTTTCAGGATTATCTTTTTTAATTGTTTCAAGATCATAAATAGGGAAGTCTCTTAAAATAGCAATGACACCTTGATGGTTATCATTACGATAGTTAGCAAAAAATGAATTATCCTTCATTTCTATTTTTGCTTTGGTAATTTGTTTTAATTTTTGTGCGAGTTGAAATGAGTTGACATAAATTGTTTCAATTGGCAAGTTTACTTTTAAAGCATCTTCAACTGAATTTCTACCACACACATATAATTTTTCCATATTTAGCACCTCAATTTTTCCTAAAAAATAGTAATTCAAAAAATGTTAATAAACTCCTAATTTCATTAATTTTTTTCTAATTTTATCAGCTTGTTTATATTCTTTTTTGTCTATAAAACAGCGTCATTGGTCAAAAAGCTCGAAAGCTTTGTTATAGTTAGCTTTAATTGTTAATTGAGGGTGGATAACTTTTAAAGTATTAAAAATTATTTCTGCATTTTTTATATTTTTGGTTTTATTGAATTCTTTAATTTGTTCATTTAATAAATAATTAAAATCAGAAAAATCGCCTATTTTTAAAGAATTCATTATCTTAATAAATTCTTCATTTTTATTAATTTTTGATTTGTCAATATTTTTAAAATTAATAAAGAATTTAAAAATTAATTTTTTGTATTTATTCAAAATAACATACATATTTTCAATTAATTCATTTGTCACATTAATTGGAGCTGAAATTTTTGAACTCAAAATAATCAACTTCAAAATTTCGCGATCATGTTCTTTTAAAAAGTCTTTTGCAAGAATAACATTGCCTAAAGATTTTGACATTTTTTCGCCCTCATAATTAATTTGTCCACAACGAATTCATTCTTTAGTTATGTCTTGATTATATAAAGCAAAGTGTTGAATATTTTCATTTTCATGATGAGGAAAAGTTAAATCCATTCCGCCTCCATGAATATCTAAACCTTTGTTTTTAAAGTGTTTATTAATCAAAACCGCACATTCAGTATGTCAGCCTGGGCGACCTTTTGAAAAAGGTGAATCAAATTGAATTCCAACATTAGTTTTCTTTCAAAGCGCAAAATCTGCTTCAAATTTTTTGTTATTATTTTGCTCTTCAAAAATCATTTTGTCAAGATTTAAATTTGCTACTTGACCATAATGATTTTCAAACTTTTTAACATCAAATCAAACATTACCTTCTTGGTCAATATAAGCATTATTATTTTTAACTTGCTTATTAACATATTCAATAATTAAGCCCATATTGTCAGTAACTTTTTCAAGATGACTTATAGTGTCAACATCCAAGGTTTTTAATAATTTCAAGTAGTCTTTAGTATATTTTTGACTTAATTCTTTTTCGCTTATATTTAGCTCTCTAGCACGATTGATTATTTTGTCATCAATATCAGTAATATTGTGAACAAAAGTGAATTTTTCTCCTAAAACTCGTCTAGCTTTTAGCATTAAATCAAAAGTTAATATTGGAACCATATTTCCAATATGTAGATCATTATAAACAGTTGGACCGCAGACATAAATTTTTAACATTTGTTAAATTATAATAAATAAGTTAAAATATTAAATACAAATAAATATATTTAAAGATATATTCAGGGAGCAATATGGTTATAGTTCATAAATTGAACAAGTTAATTAAAAACATAGTAAATGAACTAAAAGCTGAAAACAAGATTTTCAGTTCTTTTAAGTTTCTTGATGGTGAAAAGAAGTTTTCACTAAAAATGGCTCGTAAAACTAAGGGCCATAACAATAATATTAACCATATTGAATGCGATATTGTTTTTCAACTTAAAAAATTTAGTTTTATTCCTTATATTGAATTAGCAGAAGAAATTAAAAAGAGATTAATCAAAGATTCAATGGTTAAAGATGTGACAATTGATCGTTATGGGCTTTTACATATTGTTTTGAATGAAAAAGATTTAGTTTCAGTGGTTAATGAAGTTATTAAGAAAGCTTATAAATATGGACAAGAAAAGAAAAATAATATTAAAGTTAATATTGAATATACTTCAGCTTCGCCACTTAATTCTTTAACTTTGATGAATGCTAGAAATGCTTTAGTTGGAGAAACTATAGCAAGAATTTTAGAATACTTAGGTTATCAAGTTACTCGAGAATTTTTAGTTTATCCAGGTTTAAAGAAAATGGAAAAAGTAAGCCAAATTGTTCATGAACAATATGTTAACTTGTTTGAATTAAATGACAATTCTATTAAAGTTGACAAGGATATTTTAGAATGTGCAAAGAATATTAAAGCACAAAATGGTGATCTTTATTTAGACATAAATGACACAATTAAAAGAAATAAATTTGATTTGGCAATTGTTTTTTATTTCTTAAATAAATCCAAAAAACAACTTAAAAATTTAGGTGTCTTTTTTGACATAGTGAGCTATTTTGAAGACATTTTGGTCAAAGAAAAGATTGACAAAGTATTAGAATTATTGTCACCTTTTATTTATGATAATGATTCAAATTTAATACTTAATACAAATCTTGATGATAGTAAAGAACGCATTCTTGTTCATAGAAATGGCACGAGTTCATATTTATTATTAGACATAATTTATCATCTTGAAAAATTAAAAAGAGCAGACTGAATTATTGACATTTTAGATGCTAACCATACTCAATATTCACACTCTTTAATTTCTGCTTTAAAATTCTTAAATGCTTACAATAATAATATTGATATTGCCTTTTGTGAAAACTTCCAAATTAAAGAAAATAATGAAGTTAAAAGTATGACAGAAAAAAACCAAAACGTCATTTTTATAGATGAATTAGTAAAAAAAGTTAATTTAAATGAAATCAAATTTTTCTTTTTAGAAAAGAGTACTAAACATCAAATAACAATTGAAAAGGAAATTTTAAATCAAAAGAAAATTAGTCATAATTTCGATTTCATATTAAATACTTATAATAAAATAAATAAATTATTAGATGAATCAAAAAACAATAAATGTTATTGTAACAAAATGAATCAAAACAATTTTTTAAGCAAACTTATTTTAAGGCTTGATTATTTTCCTTATGTTTTAAGAAAAGTAATCAAAACTTTAAATCCTTTTTACTTAACAAAATATCTAAAAGAAATGTGTTATTTGATAAATCAAAATCTAAAAGATAAACTTAAAAAAGATTATAGAAACTTCTATCCAATTTTAGAAGCTGTCAAAAATGTATTTGATAATGGACTAAATTTACTTGGAATAGATTTTAAAAATTAAAGGCATTTTCAATAAAGAAAGTGCTTTTTTAGTGCAAAAAGTGTCAAAATATGCTGAATTTACTATTTTTTTTATAAAAATTTGTACAAAATTTAAATAATTAATTATAATTCAAATACTTAATATATAGATTATTAGGACAAGCGAGGAAATAAATTATGAAAAGACTTAATCTTTTATGAAAACCTAAAGAAGATGAAACATATCCATGACTTCTTAAACACCCTAAAATTAAAGAAGGGCTTGCAAAATTTAAAACACGTCAAGATGCTGTAGACTGATTCTTGAACTTTGGAGATGAAGTTTACATTTGATTTCAAAATAACGAAGGTATTTTTGGTGGACAATTAGCAGTATTAGAATCAGAAAATCCAAAAGACAAAGATTCTAAAAAATTATTATCAATTCCTAAAGTTTCAGGTTTTGATGGTGGTGAAACATATAACGGTATTTGTGAAGAATTTAGTATTCACCCATCATATCTTTCAAGAGAACCTTACAAATCGGCTTTAGCTAAAAGAATTCCAGAAATTGACTTTGTATTAATTTCAGATCCTGCTACATACTTCCCAGCAGATCTTGAAATGAAAAAACGTAGTCAATCATCATATGTTGACCTTAACGCTATCAAAGCATCTCTTGAACTTAAAATTAAAGAACTTGAAGACCAAAAAAATGCTTCAGATGAAACAATCAATAGCTTAAAAGAACAATTAGAAAACAAAAAATTCGACTTCATGAAAGTTGAAGAAGAAATTGAAGCTATTAAAAACCAAAAAGATCAAGAAATCAACGCAGTACGTGAAGAAGCTGAAAAGAAAATTGAAGAAGTTAAAGCAGAAGCAGCTGCAACAGCAGAACAACCAGCTTATGATCCAAATGCTGAAGTTCAATACGATCCATATGGAAACCCAATTGATCTAAATGCACAGCCAGCAATGGACCCTTATGGAAATCCATATGTACAAAGTGAAACAATTATGGCAGATCCTTATGGAACAGAAGGCGGATATTTAGTACAACCTGAAGCCTATGGGCAATTAACTCCATATGAAGCTAACTATGGATACCCAACAGGTATGTATGATGGTACTCAATTCTACCCTGTTCAACGAAAATCAGACAAAGGAATTTGAGTAGGTGTTGGTATTGCTGCAGCAGTGTTGTTAATTATCTTAATTCTTGATATATTAGTGTTACTTAGCTTAACAAATGTTGCTCAAATCTTTTAATAATCATTAAATTTAATACAAATTAACTTGCAAAAAATGCAAGTTTTTTTATTAATAATAAAACTATGGTAAAAAAATATTTTTTTCATATTACACTTAAAACAAAACATTTTTAAATAAAATAAAAATGTACAAATACAAATTTTTATTACAGAAAAGAGGAAATATGAAAATTATTGTCATTGGTGCAAACCATGCAGGGACCAGCTTTTTAAGAACATTAAAAACTGTAAATCCTAAAGCAGAAATTACAGCTTATGACCGTAATACAAACACATCATTTTTAGGTTGTGGTATTGCAATTTGAGTAGGTGGAAAAGCTAAACAACCAGATGGATTATTTTATTCTTCACCAAATATTTTGCAAAAAGAATATGGAGTAAATTTAAAAACTTCACATGAAGTTATCGAAATTGATAGAAAGAAAAAAGAAGTTACAGTTCAAGATTTAGATAGTGGTAAAACCTTTAAAGACACATATGATAAATTGGTTTTTGCAGGTGGTACATGACCTATTGAACCAAAGATTCCAGGCATTGAATTAGACAACATTCTTTTATCAAAACTATATCAACATGCTCAAGTTTTAGTTAAAAAAGCAAATGATAAAAAGATTCAAAATGTTGTAGTTGTCGGTGCTGGATATATTGGAATTGAATTAGTAGAAGCATTTCACAATAAAGGCAAAAAAGTTACTTTAATTGACTTAGCTGATCGTGTTGTTCCGGGATACTTAGATGGTGAATTTACAGACATCATGCAAAAAAACATGAAAAAAGAAGGTGTTAAACTTGCTCTTGGAGAAAAAGTTGTTGAATTCAAATCAAAAGATGGAAAACATGTATCACAAGTTATTACAGATAAAAGTAAATATGATGCAGATCTTGTAATTATGTCAGTAGGTTTCAAACCTAGAACATATGTATTGAATGATGTTGAAAAATTACCTAATGGAGCAATTAAAGTTAATGAATTCCAACAATCAATTTCTGACGAAAACATCTATGTTTTAGGTGATTCTGCAGCATTAAAACACTGTGTAAGCAACACATATGAACACGTAGCTTTAGCAACTAATGCAGTTAAAACAGGTATTGTAGCTGCATTAAATTTAGCTGGACTTAAAGTGCCATTTCCAGGAGTAGTAGGAACAAATGCTATTAATGTTTTTGGTTGTCACTATGCTGCAACAGGATTTAATGAAGATGCAGCTAGAGCTCATGGATTTGATGTAGCTAGTGAATATTTTGAAGACAATGACCGCTGCGAATTTGCTGGTAAAAGTGAAAAAGTTGGATGCAAAATTATTTTTGACAAGAAAACATTACAAATTTTAGGTGTTCAAATCGGATCATGAGGTCAAGTAAATCATACAGAAGTTATTTACATGTTTGGTTTAGCACTTCAAAGAAAATTGACTCTTCCTGAATGTGCTTTAACTGACGTCTTCTTCTTGCCTCACTTTAACAAACCATTTAATTTCTTCTTAATGCCAATGCTTAAAGCACTTGGACTTAAATATAAAGCTTAATGATTAAAGCAAACAAAAAACCAGAAGTTTCTGGTTTTTTAATTTATAGTTCAGAAGCTAATTATGCATCTTTTTTAGCATTTTGTTCGTTAACAAATTTGTCTAAACGAGATGATTTTCTTGCACCTTTATTTGGGTGGAAAACACCTTTTTGAACAGCTGTAGCAATAATTGAGTGAGCTTTATTAACTAACTCTTGAGCGTTTTTATCTTTAGCTAAAATTGCTTCTCTAGCTACTCTAATTGCTTTACGAACTCTTGTTTTCATAGCATTGTTACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGCCTAATTTAAGAATGGTTGGTAAAATGTTAGAAAACGTAGAAGAGAATGGCCACAGCTTAAAAAATGTATTATTACATTCTGATCAAGGATGACAATACACTCATCAAGATTATATTGATTATTTGAAAGAAAAACAAACAACTCAAAGCATGTCAAGAAAGGGAAATTGTTTAGACAATAGTCCTACTGAATGTTTATTTAGTGTTATAAAAAGAGAATTTTGATTTGGAGAAGAAAAGAAATTTAATAGTTTTAAAGAATTTAAAACTGCTTTAGGAGAATATATTTCATATTATAATAATGACAGAATTGTTAATAAATTAAAAGGACTTAGTCCTGTCCAATACAGGAATAAGTCCAAACATAATTAAAAAGTCCAATTTTTGGGGTTCATACCACAAAAAACAAAGATTTTTATTTTTTTTAATAAATTATGTAAAAAATGGCTAGGTATATATAGGAGGAGAAATGAATGAATTATTAGTTTATTTAAGTTATAAATATGAAGGGAATATTTTTGAAATTTACAAATTTTTGAAAAATAAGAAAAAAATTAATCAAGCAGAAATAGATATAGTATTAAAGGAGCTAGAAGAAAAAGGTATTAAGTATATAACAGCTTTAGACGAAAATTTTCCAGAGATATTAAAAAATTTTCGTTATTCGCCATATGTTCTTTTTTATAAAGGCAATATTGGCTTATTAAATAAGAAAGCAGTTTGCTTAACTGGAGATATTGAAAGTCCAATTGTGGACATCAATATTAAGAAATCACAAAATACATTTTTGGAGAATTATCCTTTAATAACGAATGATTTCAAGAATTTAGATCAAAAATTTGTTGAAATATATCGTCAAAATAAAAAAGAAATAATCCATGTATTGCCTTATGGATTAGATTATATAAAGGAAACTATAAATTTAGATACAGAATTGTATATTAGTCAATATCCACCACAAACTCATGCTAAATTATTTAGATTTAAAGAAAGGAACATTTTAGTTTCATTTTTAGCTGAATTTTTAATTGTTTATAGCAGCAAAAGCGACTCGGGTATTTTGAGTCTTGCAACAGCTTTTGCTAACAATAATAAGGAAGTGTATTGTTATCCGGGTTTAAGTTATGATGATGGAAACAATTATTTAATTAAATTAGGAGCTAATCTAATTACGCAAATTGCAGAAGTACAATATTATTAATTTTTGTCGTCTAAAATTTCATTAATTGCTTGACCAATAGCGCCAGTTAAATTAGAACCTATAACTCTATCGGCAGCTACTTTTGCTTCTTCAGTGGCATTTCCCATTGCAAAACCAATGTGAGCATTTTTTAACATTTCAATATCATTGCCACTATCACCAAAAGTATAGAGCTCATGAACATTAATTTTTAAATAGTCTAGAAGTGAACTTAAACCATTTCACTTAGTTACACCTAAAGGATTAACATCAGTGTAAATTGAGTTTGTTAAGAAAACTTTTTGTTTATCTTTTAATTTATTTTGAAAATACTTTGTGGTTTCTGCTGCAGTAATTAATGGATTTCTTAATGCCATTTGAGTAACTTTACTATTATTATCTTTAAGAACTTTTTCTAAATCTGAATATGGTTTTAAGAAATTTGAATTTTTATCTGTTAATTTTGTTTGATCTTTAACTCATTCTGGTAATATATTTTTATCACTATTTCTAGATCATGCACCATTAAATTCAGGAGTATCAATAGTTAAAATTAAATCATGAGCTTGAGCATAATCAAAAAGATCATTAAATATATCTTTATTAACTTCGCCTAAAACTTTATAATAATCTTTTTCAACATCATAAAATAATGCACCATTTGAACATACAAAATAATTAATGCCTTTAATTGATTTTAAAAGTGGTAAAGTTTTTTGCAAACCACGACCAGTACAAATGATATTTGCATGTCCTAAATTATATGATTTTAAAATTGCTTCTTGAGTTTCTGGATGAGTATTAACTCCATCTGTTAATAATGTGCCATCCAAGTCAAAAGCAAACACTTTTCTTTTGTTTTGCATAATGCGCTCCTTACTATATTCATTAATTATATTATTTTACCATTTTTTTCAATCTTTTTTAAAAAAGACTATTAAGGCAAATTATAAACCAGGACAAAAGAATTAGACTTAAATCCCAAAAAGATTTAAGTCTTTTTGTACTAGTTTATTATTCTAAAACATCTGTTTTATAGGCAAAAAATAAAAAAGCATGAATGCTTTAATATTTGAAATATGTGAACTTGTATCAGAATGGTAAGAAATCATATTTTGCACTTTGACTAAATGCCACAAATAGAATCAAACCAACTATCAATATAAAGAATAAAATATCTAATCAACCTATTCTTAATTTACGATATCTTGTTCTTTTTGCATAAGGATCATAACCCCTAGTTTCCATAGCATTTGATAAGTCTTCCGCTTTAGCAAAAGAAGTAACAAACAATGGAATAATTAATGTTGTAAATGATTTAACTTTTTCTTTAATTTTTCCATTCTTAAAGTCAACTCCACGACTTGCTTGAGCTTTCATTATTCTTTGTGCTTCTTCAAAAAGAGTAGGAATAAATCTTAATGCAATCGAAATAATCATAGCAATTATGTGAGTTGGAATGAATAGAAGTTTAAATGGAAACATCAAGTCTTCAAGTGCTTTAGTAAGTAGTATTGGTTTGGTTGTAATCAATAATAAAGAAGTAACCATAATCATAATGTAAATTCTTATAATTAATGCTCCAGTTCTTGCTAAAGCTTCTAAAGTTAAGGCATATTCACTATGGCCACCTCAGGGTATTATTCTTGTATAGTCCGAGTCAGGTTTTAACTTCATAGTATAGATATTAACAAAGAGAATAAAGAAACCAATAATTAGAGGCATAATCATTAATCTAAATAAAGGATATATTTTTCTTGAACCAATAACAAATGCAATCATTAGAGGTACTAATAAAACAGCTAAAGAGACAAAGTAATTAACAATAAAAGTTAAAACAATATAAATAATATTGATTATCAATTTTAACCTTGGATCAATTTTATGAATTATTGTTTCATGTGGATAATAACGACCAATAGGAGATTTCATTATACACCTCCTTTCATTCTTTTATTTAAGAATGCAGCTAGTTCATCAATAGATGTAACTTTAGGCACACTAATGCCTCTTTCCTCTAATTTAGAAACGAATTCTAATAATTTTGGAGGTTCCATATTGTTTTCTTTTAAGAATTTTGTATTCCTTAATACTTCATAAGTATCACCATCTTTAACAACTTTTCCATCTTTAAAAATTAATACTCTTTTTGCTACTTCTAAAACATTATCCAAATCATGTGTAATAATAATAACCGTTTTACCTTGTTGATGAAGTTTATTAAAAATTTCTAAAACTTCTCTAACTCCAACTGGATCTAATCCAGCTGTTGGTTCATCAGCAATTAAAATGTCGGGTTCCATAGCTAAAATTCCAGCTAAAGCAACACGCCTTTTTTGACCACCACTTAAACCAAAAGGTGATTTATTCAAGTAACTTTCATCCAAACCTACTAACTCAAGATATTTTTTAGCTTTTTCATAAGCTTCAGTTTTATGCACACCAAAGCTTCTTGGTCCAAAAGCAATATCATCTTTAATAGTAGATTCAAACAATTGGTATTCAGCAAATTGAAATACTATTCCAACTCTTTTACGAATATCTTTAGATTTCTTAACTCTTTTTTGTCTTGCTTTAACAATATATGAATCAATAAATTTTTCTTTTTCTTTTGTTTTCTTATTTATTTTTTCATTTTCAAAAATTCATTCAATTGAACCATTTCATGGCAAAATAAGAGCATTTAAATGCTCTATGAATGTAGTTTTACCTGAGCCAGTTTGTCCAATTATTCCTATATATTCGCCTTGATTGATTGTTACATTAATACCACTTAATGCTTTAAATTCCATAGGAGTTCTACGACTAAAAATATGTTCTAAATCTTTAACTTTTATTTGCATAGTTGATTTAATAACTCCTCTTCATTATATGTTGATTCAATATTTTCAATTTTTTCACTGAGCTTATAAATAAAAGGTGAATCAATTTTAGCTAATTCAATGATTTCATGATTTTTTAAAATTTCTTTAGGTGAGCCACTTGCAACAACTTGACCTTTAGAAAAAACTAAGCAATAATCAGCTTGAATAGCTTCATCCATATCATGAGTAATTGAAATTAAAGTTTTTTGACGAGTTTTTTGGATTTCTCTAATAATTTTTAAAACTTGACTTTTACCTCTTGGATCAAGCATTGAAGTTACTTCATCAAAAATTATGATTTCAGGATTTAAAGCTAAAACAGAAGCTATGGCAACGCGTTGCTTTTGACCTCCTGAAAGGAATTGAGGTTCTCTTGTCAAATAGTCCTTCATTCCTACTTTTTCAGCTAAATCATCAATAATAGGTTTCATTTCTTTTTGTGGAATGCATTGATTTTCTAAACCAAAGGCAATATCATCTTCAACACTAGCGCCAACAAATTGGTTATCAGGGTTTTGGAAAATAATGCCAATTTTCTTTCTTATTTCTCTGATTGTTTTTTTAGAAATAACTGTTCCATTTATTTCAATAGAACCTGAAGAAGGTTTATACAAAGCAACAAGCAATTTACTGAATGTACTTTTACCTGATCCATTGTGACCTAATATAGCAACATATTTACCATCTGGAATGGTAAAATTAAGCCCATTGAGAGCTTTTGTTTTTGATTCTGGATAAGAAAAAACTAGGTCCTGTACTTTAATCATAAAAATATTATATTATGAAATGATTTTTTTAAAAACCAATAAAATATTTTTTATTTATAAAAAACAAAAATGTTTAATTTTTATTATTTCTAAAATACTAAATTCTTGAAGATATAATTCAACAATTTTTATCTTTTGATCAAGATTTAATTTTAAATAGCCTTTTATTTCTCTATTTCATTTGTTTCTTTTTTAAATTTGAATTGCATAAAAGTGAACCCCTTTCCAAAAAGTCTGGAATTTGGGGTTCACTCTAAATGTGCCTTATTGTGTGATTAAAATAATCAAATTATGTATGTTGAATTTAGTCTAAAACAGCTATAAAACTGTCTTATTGGTAAAAATTATTATTTAGTTACTTTAGCATCTTTTTTGCTTTTTGTTGCTTTTGATGCTGTAGTTTTTGTGTCTTTGTCTTTTGATGTTTTTTCTGCTTTGTTTGTTTTAACTTTGTCGCCTTTAACTAATTCAATAATAGCCATAGGTGCATTGTCTCCATCACGTGAAACCAATTTGTAAATACGTGTGTATCCACCATTACGGTTTAAGTAACTTGGAGCAATTTCTTCAAATAAAACTCTTAAAACATCTTTACCATCTGTGTTTTTAATTGTACGTAAGAAACTTGCAGCAATACGACGGTTTGCTAAAGTTGGGTTTTTTGCTTTGGTAATTAATTTTTCTGCATGGCGACGTAATTCTTTAGCACGAATAAGTGTTGTAGTTAAACGTCCGTGGATGAAAAGTTCACTAACTAAGGTACGAATAACACCTTTTCTTCATTTAGTATCACGACTATAAATTTGTTTTGGGTTAGCCATTATTCTTCTCCTTTACTTAGTTCATATCCATATTCTTTTAAACGTTGTTGAATTTCATCAAGTGATTTACGACCAAGGTTTTTAACTTGTTCTAATTCTTCAAGTGACATTTCAGCTACTTGAGAAATCTTTGTTTTACCAATTCTTTTAAGTGCATTCAATGAACGTACCGAAAGATTTAATGAATTGATATCAACATCATTGTCGTTTGTTTCTTGAGTTTGTTCACTTTCTTCTGCAAATACATTTACTTTCATTTCATCAACATTACCAATCAATTTGAAGTGACCAACTAAGATTTCACAAGCTTGTTGTAATGCACTCTTAGGATCAACTGTTCCATCTGTTTGGAATGAGAAGGTTAATCTTTCTTCAATTTTGTTTGATGATGAGTTTAATTCTTCAACTGTGTAGTTAACTTTTTCAATTGGACTAAAGTTACTATCTGTAGCAATAAAAATTCCGTTTTTGATTTTTGATTCCATTCTAGTTGTAATTGAAGAGTTATTAAGTAATTTCTTATTTTCTTCATTAGAAATAAAGCCACGACCTGGTCTTAAATATAATTCAATATTTAATTTGTGTTTACCATTTGCTGATAAGTGAGCAATTTCTAATGATTTGTTTAAAATTTCAATACTTGAGTTGTTTACTTCTAATAATCTTGAAGTAACTTCTCCTGGTTGATCAGCTTTTAATGTTACTTTAATAATTTCGTCATCTTTAACAAATTCTGGGTTGTATTGGAATTTTACTTTTCTTAAATTCATAATAATTGTGGCAACGTCTTCTACGCAACCATCAACTACTTGGAATTCATGATTAACATCTTCAATTTTGATTGCAAATAATGCTAATGAAGTAATGTTTGAAAGTAAAATACGACGAAGAGCAACACCTAATGTTTGACCCATACCACGTTCAAGTGGTTGAAGAGTAAATGCAGTTACATTTGAAGTTGAATTATTTAATTTAGTAACCTTTTGGTACTCTAATTTTGTCATTTTTTCCATAATTATTCAACTCCTTTACTATTTTTTAGCTTCACGTTTAAGAATACGTTTTGGAGGTCTTGTTCCGTTGTGTGGAACTGGTGTAACATCTCTAATTTCTGTTACTGTGATACCGGCAACTTCAATTTGTTTTCTAGCAGCGTCTTTACCTGCACCTAAACCTTTTAATTGAACTTTTACGCTTTTAATACCATGTTCTTTGGCAGCTTCAGCAGCTGCTTGTGCAGCTAAACCAGCTGCATAAGGGGTTTTCTTTTTGGTCCCTTTGTAACCAATAGCACCTGAAGAACTTCATGCAATTACGTTTCCTTTTTCGTCAGCAAAGGTAACAATAGTATTTTGGTTTGTTGAGTGAATGTGCGCTACACCATTTGTTATATTTTTCTTTTTGGTTTTTCTAGCCATAATTAATTCCTCTTCCTATCTATTATTTAGTTGATTTTTTACCTGCAACTGTTTTACGAGGACCTTTACGTGTACGAGCATTCTTTTGTGTACATTGACCTCTAACAGGTAAACCTTTTCTGTGACGAATACCACGGTAGCATTTGATTTCCATTAAACGTTTGATGTTTAAACTGGTTTCACGACGAAGATCACCTTCAGTTGTGTATTCTTTAGCTACTTCACGAATTGCTGATAATTGTTCTTCTGATAAATCTTTAACTCTAAGATTTTCATCAATTTTTGCTTTAGCACAAATTTCTTGTGCTCTAGTTCTTCCTATACCATAAATGTATGTTAATGAGATAACAACACGTTTATTGTTAGGAATTTCAATATTTAAAATTCTAGCCATTTAATTCCTCTTATCCTTGTCTTTGTTTGTGTTTTGGTTGTGAGCAAATTACCCGAATAATTCCTTTACGTTTGATAACACGACAATCTTTACACATTCTTTTTACACTAGCTCTAACTTTCATATAATTCTCCTATAAATCTATTTGTGACGATATGTTATTCGTCCTAAAGATAAATTGTAAGGACTAATTTCAACATCGACAGTATCTCCAGGCAAAATACGAATGTGATTTACACGCATTTTTCCTGAAATGTGAGCTTTAATGAGCATGCCGTTTTCAAGTTCAACATCATATTCATCTGTTGAATAAGCTTCTTTGACAACAGCTTTCATTTTGATTGCGTCTTTTGCCATTTAGATTCCTTTCGTTAATATAATACCTTTGCCATCTTTAATTAAAACAGTGTGTTCGTAGTGAGCTGCATTTAAACCGCTAGCGCTAACAACGGTTCATTTATCTTTTTTAACTTTGATTCTAGCATTTTGTTGTAAAATCATTGGCTCAATACAAATTACCATTCCATCTTTTAATAATGGTCCACTACCAGCATGGCCATCATTAAATACATAAGGGTCTTCATGAACTTCACGTCCAATTCCATGCCCACAGTATTGACTTGGTGTATATAAATTATTTTGTCTTATTACTTGACCAATAGCGTGACTAACATCTCCCACTCTTGCACCAGGTTTAATCGCATTAAAACCTGCATAGAATGATTCTTGAGCCACTATAATTATTTTTTTATCACTTTCATTGATATTACCAACACCTTTAGTGAAGGCACTATCGCTGTTCATTCCTTTGTAAGTACATCCCATGTCGACTTTAACTATGTCGCCATCTTGAATAATGTAATCACTTGGAATTCCATGGATCAATTCTTCATTTATTGAGATGCAACAGGTTGCTGGAAAACCATATAAGCCTTTAAAAGCTGGTTGTGCACCTCTTTTTACTATTTCATTAAAAGCGACTGAATCCACTTCTTTTAATGAAACCCCTGGTCTTACAAAGTCATAAACTATTTGTTTAACTTCTGCCAGGATTTTGCAGCTTTGAGTAATATTAATAATTTCTTGTTCATTTTTAATAGTTATCATAAGCAGCTTCTTTCTACTTTTAGCAAATTAGTTTTTTAATTTCGCTAAAGATTCAACAATATTTTTTGCTATAGTTTCTGGATCACTTGTAGCATCAAATGTTAATAATAAATTATATTTTTTGTAATAATCAAGCAATGGTGCTGTTTGATCATGATAAACTTTTTGACGTACTTTAATAGCTTCAATTGAGTCGTCTTTTCTTAAGATTAATTCAGCTCCACATTTATCACAATATTTGCCTTTAGTTGAAGGCATAAAATCAATATGGAAACTTGCTTTACATTTAGGACATTGTCTACGTCCATTTAATCTTTTTAAAATAACATCTTCAGGAGCACTTAATTCAAGAGCTACATATTTAAAGTTTGAAATACTATCTAAAAAGTTAACTTGGTCAATAGTACGTGGATAACCATCAAGCATAATAATCTTTTTAGCTTTGATTAAAGCTTGAATTTTATTTTTAACAATAGTATTTGTAATGTCGTCTGGTACATATTGTCCTGCTTCAACTATCTTTTTAACTTGTAAACCAAGTTCACTTTGTGAAGCTATTTCTTCACGAAAGATATTACCAGTTGAAACATGTACTAGTCCATATTTTTCAGCTATTATAGCAGCCACTGTACCTTTTCCTACACCAGGAGGCCCCATAAAAACTATATGTTGTTTAATTTTACTTATCATAATAAGCCATCTCCATTTTTGTATTTATCATATTCTTTTGAAACGTCTTCAGATTTTTGATCATTTGTTGCAAAAGGATCATTTTCTTGATATTTGTTTTGGAAGTTAAGCACATCTGCAGCTTCTTTTGAAAGACGTTTTTGTTTTGCAAGATGTTGACTTTTACGGCGTGCCATGAATTGTTGAATAGTTTCAAGAGCAACTGATACTAAAATCATCATACCTGTACCACCAAAACTTACTATAGCTGGAATTTGTAATCATAAAATCATTATGTATTGGAATGAAGCTAAGAATACTAAATAGAAACCACTGAAGGTGCAAAGTCTTAAAACAACACCTATTAAATAATCTTCAGTTTCTTCTCCAGGTCTAATACCAGGAATAAATGTTGAGTTCTTTGCAAAGTCTTCTGAAATCTTGTCAATTCTTGATTGTTGCAATCCAATTATGTAACTAAAGAAGAATGTAATTAGAATCAATAATGACAAGCCTAGTGGTTGAGTGAATTGCATATTGGCTTCAATTCATAATTTGCCAGAATTACCTTTTGGAAGTATTCTAGATATCATAATTGGGAAGCTGACTAGCATTGAGGCGAAGATTATTGGCATAATTCCACCGGGATTTAATTTAATTGGCAATTTACCCATTTCATTAATATTTCTACTACGTCCAGCACCAATTTGTTGAATAGGTACATGTCTTTCAGCATTGTAAACTAAAGCAATAATAAATAACAATGCAAAGTAAACAAATAAGTAAGTTAAGAATTTTAATGCTCCAACAAAAATTGCTGCTGTACTTTCACTACCAACAAATAAGCTAAATGCTCCTTTAAATTGACTTTGTAATTGGAATGCAATACCAACAAAAATAATTAAGCTTGTACCATTTCCTAAACCTTTATCTGTAATTTGTTCAGCAATGAATAATGAGAACAATGATCCACCAATTAAGATTAAAGGCAATATTAAGTAGATTGTAACTTCTCGAGGGAGTTTTTGAATTGGAGTAAATCCAATAAATGGATTGTCACCTAAACTAAGTGATTTAGTAAGCAAGATAGCTTGAGGGAATGCAATAAACAAAGTCAAAATTCTTGTAATGATATTTAATTTTCTCCGACCTTGTGGTCCACTTTGACTAAGTTTTTGAATTGGAGGGAAAAGTTTGGTTTGTAAAAGAGACATTATCAAGCTGGCATTAATGAATGGACTAATTCCAAGTGCTGCAAGTGAGAAGTTTTTAAGACCTCCACCGCCAACAAGGTTTAATGTATTTAAGAATGGGTCATCATTAATATTTACATCAGATAATTTAACAAAAGGAGCTTTGATTGTTGTCATTAAAATATAGATTGATAACAACAATAATGTATAAAGCAATTTTTTAGTAATTTCTTTAGTACTTCAGAAAACTATTCATTTGTTTTTGAAGTTATAAAAAAATTTTGAAATCTTAAATCCTACATTTGAGAAAAATTTCTTCATATATTCAACCCTCACTTTCATTGCAAAAACCAATTCAACTTTGTCTCTTATAAAATACTTTATTATTATATAATAAACTACCAAATTTTATAGTAAAAGTTTAATAAAAAAACTTATATTTTTTTAGCCTTAAAATAAATAAAAAATATTTTAGTTTTTTAGTATTTTAAAAAAATAAAAAAATTTCACCAAATCCTTTGTTTTATAGGCTTTTTGGTAAAAAAATGAATAAAAAAATGTCACACTCGTGACACATGTTCTCTGAAAACTGAATAGTAACAATAATTCATTGAAATGTCATAAATACACTTCTACTTTTAAACCGATCGATTTATTAGTATTGGTCAGCTCAACGTATTACTACGCTTACACCTCCAACCTATCAACCTCATAGTCTATAAGGAATCTCAAGGGAATACTTATCTCTGAGGAGGCTTCCCACTTAGATGCTTTCAGCGGTTATCCTTTCCGTACTTAGCTACCCAGCTATGCTCCTGGCGGAACAACTGGAACACCAGTGGTACGTCCACTCCGGTCCTCTCGTACTAAGAGCGGCTCTCATCAATATTCCAACGCCCACATCAGATAGGGACCAAACTGTCTCACGACGTTTTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGAACCGACTCCAGCTCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTTGCCGTCGATGTGATCTCTTGGGCAAGATAAGCCTGTTATCCCCAGGGTAACTTTTATCCGTTGAGCGACTGCCATTCCACAATGTACAGCCGGATCACTAAGTCCTGCTTTCGCACCTGCTCGACTTGTAAGTCTCACAGTCAAGCACACTTCTACCTTTGCGCTCTACATACGGTTTCTGACCGTACTGAGTGTACCTTTGAACGCCTCCGTTACTCTTTAGGAGGCGACCGCCCCAGTCAAACTACCCGTCACGCACTGTCCACCCACCTGATGATGATGGCATGTTAGAAACTCAATATACCAAGGGTGGTATTTCAAGGATGACTCCACGAGAACTAGCGTCCTCGCTTCAAAGTCTCCCACCTATCCTACACATGTTAGACCAAGTTCCAATACGAAACTGTAGTAAAGCTCCATGGGGTCTTTTCGTCTTGATGCGGGTACCCAGCGTTTTCACTGGGACCATAATTTCACCGAGTCTAGTGTTGAGACAGTTGAGAGATCATTGCGCCTTTCGTGCAGGTCAGTATTTAGCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTCACCCGGGCTTCATTTCAACGCTTCGCAAATGCTAACGCATCCACTTAACCTTCGGGCACTGGGCAGGCTTCACCCCCTATACATCACCTTGCGGTTTAGCAGAGAGCTGTGTTTTTGATAAACAGTTGCCCCTCATAATTTACTGTGGCCTAAGTGTTACCTTAGGCGCCCCTTCTTGCGAACTTACGGGGTCATTTTGCAGAGTTCCTTAACACTAGTTTTCTCGCTCGCCTTAGAATACTCATCTCGGGGACGTGTGTCCGTTCTCGGTACAGGTTTCCATAATATTAAGTTTAGAAGCTTTTCTTGGAAGTGTGGAATCATCTAATTCGGTTTGACCCTATGCATCACGCCTCCCGGTTATAGACTGCGGATTTGCCTACAGTCACCAGTGAACGCTTACCCCACAATCCAGTAAGTGGTAAGATTATCCTCCTCCGTCACTCCATCACTATTATAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTACGCTTTTCAGCCTCGTCTTAGGTCCTGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATAGGCGTCGTAGATTCTCACTACGAATCGTTACTCATACCGGCATTCTCACTTCCTAGCGCTCCACCAGTCCTCACGGTCTGACTTCATTGCCCTAGGAACGCTCCTCTAACGTAAATATAATTACCCGCGGCTTCGGTATCGTGTTTTAGTCCCGTTAAATTGTTGGCGCAAGGTCTCTTGACTAGTGAGCTATTACGCACTCTTTAAAAGGTGGCTGCTTCTGAGCCAACTTCCTAGTTGTTTATGAAACCTCACAACCTTTCTCACTTAACACGATTTTGGGACCTTAGCCGGCGATCTGGGTTGTTGCCCTCGCGAGCCGGGACGTTAGCACCCCGGTTCCGACTGCATAGCAATACATAGCGGTATTCGGAGTTTGATTATAGTCAGTACCCCTAGGCGAGGCCATTCCATATTCAGTGCTCTACCACCACTACTTAACACTACACGCTAGCCCTAAAGCTATTTCGAGGAGAACCAGCTATCTCCAAGTTCGATTGGAATTTCTCCACTATTCACAAGTCATCCGGGCACTTTTCAGCGTACTACGGTTCGGCCCTCCGCTTGGGGTTAGCCAAGTTTCAGCCTGCTCATGAATAGATCACATGGTTTCGGGTATATGACAACATACTAAGACGCCCTATTAAGACTCGATTTCTCTACGGCTCCGCTTTTTTCTGCTTAACCTCGCATGCTGTCATAACTCGCCGGTCCATACTGCAAGATGTACGCCATCACCCATAAACGGGCTCTGACTAATTGTAAGTAAGTGGTTTCAGAATCTATTTCACTCCCCTCTCGGGGTTCTTTTCACCTTTCCCTCACGGTACTAGTTCACTATCGGTGTCTGATTAGTATTTAGCCTTACCGGGTGGTCCCGGCAGATTCAGACAGGGTTCCACGTGCCCCGCCCTACTCAGGATACGATCAGAAGACTTAACAATTTCACATACGGGGGTATCACCCTCTATGCCGCTTCTTCCCAAAAGCTTCTGCTATCATTAAGTTTTGTAACTTCATGTAGATCGTCCTACAACCCCCATTGCTGGGTTTGGGCTCTTTCTCGTTCGCTCGCCGCTACTAAAGAAATCATTGTTTATTTTCTCTTCCTCTTGCTACTAAGATGTTTCAGTTCACAAGGTGTCTCACTCAAGTTCCTATGAATTCAGAACAAGGCAACTAGGCATTACCCTAGTTAGGTTTCCCCATTCGGAAATCCCCGTTTCATAGCATATTTCCGGCTCCACGAGGCTTATCGCAGGTAATCACGTCCTTCATCGACTTTCAGACCCAAGGCATCCACCACAAACTCTTCCTTATTTAAAAGTATTTTCCTATTGTTATAGTTTGTTATGATGTGTATTTTAAGACATTTCAATGAATCATAATTAATATGATTTTCTCGATGAAATCAAAACAAATTAACCATTTAATTTAATTTTGATGTCGTTGTAAATATCAAAAAATTTACTATTCAGTTTTCAAAGAACAAATGAGAGAAAAAAATATTGTTCTCTCAAAACTAGATATAAAGCTTTAGACCCATAAAAAAGCCACATAACAAAAAATATATAAATAAAATAGGTCTAGTGAAATAAGGTTAATAGTACTTTAAAAAGTACTCTTTTGTACTCCGTAGAAAGGAGGTGATCCATCCCCACGTTCTCGTAGGGATACCTTGTTACGACTTAACCCCAGTCACCAGTCCTGCCTTAGGCAGTTTGTTTATAAACCGACTTCGGGCATTACCAGCTCCCATGGTTTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACCGTAGCGTAGCTGATCTACGATTACTAGCGATTCCGACTTCATGTAGTCGAGTTGCAGACTACAATCCGAACTGAGACCGGTTTTTTGAGGTTTGCTCCATGTCACCACTTCGCTTCTCTTTGTACCGGCCATTGTAGCACGTGTGTTGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCCCCACCTTCCTCCCGATTACTCGGGCAGTCTCCTTAGAGTCCTCAACTAAATGGTAGTAACTAAGGATAAGGGTTGCGCTCGTTGCAGGACTTAACCAAACATCTCACGACACGAGCTGACGACAACCATGCACCATCTGTCATTCTGTTAACCTCCACTATGTCTCCATAGCTTTGCAGAAGATGTCAAGAGTGGGTAAGGTTCTACGCGTATCTTCAAATTAAACCACATGCTCCACCGCTTGTGCGGATCCCCGTCAATTCCTTTAAGTTTTATTCTTGCGAACGTACTACTCAGGCGGATCATTTAATGCGTTAGCTGCGCCGATGAGTTCCCCATCAGCTAATGATCATCGTTTAGGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTCTCTCAGTGTCAGTATATGTCCAGTTAGCTGCCTTCGCCATCTTGGTGTTCTTCCTTATATCTACGCATTTCACCGCTTCACAAGGAATTCCGCTAACCTCTACACAACTCTAGCCTGCCAGTATCCAACGCGTTTTGGGGTTGAGCCCCAAAATTTAACGCCAGACTTAACAAACAACCTACAGACGCTTTACGCCCAATAATTCCGGATAACGCTTGCAACCTATGTATTACCGCGGCTGCTGGCACATAGTTAGCCGTTGCTTTCTAATAAGGTACCGTCAAAACAAAATCATTTCCTATTCTGTCTTTTCTTCCCTTACCACAGAAGTTTACAATCCATAGGACCTTCATCCTTCACGCTGTGTCGCTCCATCAGGCTTTCGCCCATTGTGGAATATTCCCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGTATCTCAGTCCCAGTGTGGCGGTTCAGTCTCTCAACCCCGCTAAACATCATCGCCTTGGTGGGCCGTTACCTCACCAACTAGCTAATGTTACGCACCCCGCTCCTCCAGCGAAGCAAACGCTTCTTTTCCGTAATCTTCATGCGAAAACTACGAGTATTTGAAATTATCGACTGTTTCCAGTCGCTATGCCAAACTGAAGGGTACGTTGAGCACGTGTTACTCACCCATTCGCCGCTAAGGTATTGCTACCTTCGCTCGACATGCATGTATTAGGCACACAGCCAGCGTTCATCCTGAGCCAGGATCAAACTCTCGAAAAAATTGACTGTCATGTTTATATATCTAGTTTTCAAAGAACAATTGCGCACTCTATGGTTTATAGATTGCTTTTTAATTATATATAATAAAAAATTAAAGAGAAGAAAATTTTTTTATTTTTTTTCAAAAATTTTGGCAAGTTAATTTTAAGAGCAAAAAAACTAATTTTTTGCCTATAAAATAAGATATTTTGGTTACTTTAATAATTTATTTAGTTAGCTAAATATTGCAAAAAATATGAAATAAAAAAGCAAGGTTACTCCTTACTTCTTTATATTATTAATAGAAATACTTTTTTATAGACTAACAATAGTTAGGTTGCAAACTATAATAAAGTAACTTTTCCACCTTTAGCTTCGATTGCATCAATAGCACTTTTTGAAGCTGCATGAAGATTAACATTGAATTTTTTAGTTACTTGACCATTACCCAATAATTTAACAGGCATTTGTCTTTTAATCAAATTAGCTTTGAATAATGCTTCTAAGTCAATAGTTGCATTTGCTTTAAAGTTGTTTTCTAAATCAACTAAATTAATAATTTGGTATTCGATGTGGTTAACATTGGTAAATCCACGTTTTCCAATACGACGGAATCAAGGTGTTTGACCACCTTCAAATCCTAGTCTGTGACCCTTACGTTTGTTTTGTCCACTTTGACCTTTACCAGCTTGTTTACCTTTTCCAGCAGCATGTCCGCGACCAACACGGTGTTTTTCAACACGTGAGCCAGCGGTAGGTCTTAATGATTGTAATGTTGAGTTCATTTTAGTCCTTTCCTTTATGTAAATAAATTTTTAATTTTACTTAGTCTAAATTAATTAGGCTAAAAGATCTTTAACATCTTTACCACGAATGTCAGCAATTTGTTCTGGTGTTCTTAAAGCTTGAAGAGCTTTTAAAGTTGCTCTAACAATGTTTGCTTTTGAACGTGAACCATAAGTTTTGGTATAAATATCGGTGTAACCTGCAAGTTCAACAACTGCTCTAACTGTACCACTAGCAACAATACCTTTACCTTTTGTATCAGGTTTAAGCATAACTCTTGAAGCTAAGAATTTAGCATGAACTTCATGAGGTACTGTGATTTTGTTTTGAATTGGAACTGTAATAAGGTGATTTTTAGCATCTTTAACTGCTTTTTTGATTGAGTCAGGAACTTCGTTTGCTTTTCCATGCCCAAAACCAACACGTCCTTTTTTGTCACCAACAACTACAAAAGCTGCAAATGAGAAACGACGTCCACCTTTAACAACTTTAGTTACACGTGAGATATTAACAACTTTTTCGCTGAATTCATTGTCGCTAGCATCAAAGTGATTACGACGGTCACGGCGATTGCCTTTTTCGAATTTACCTTGTGGACGAGAGTTTTGAGAAATTTTTTGACCTTGAGGTCTTTTTTCTCAAATTTGTTTTTTATCTTTGTTTTCAGCTTTAGCTTTAGGAGCTACAACTTCTTTAGTTCCTTGAGCTTGGGCTTCTTTAGGTTGTTCTTTATTAACAACTTTCTTTACTTCTGCTTCTGCCATAATTAGAATTTAACTCCTTTAGCTATACTTCTAACACCTTCTGCGAAGGCTTTAACACGTCCATGATAAATGTAACCTGAACGATCGAATACTATTTCTTTTAATTTAAGGGCTGCAATTTTAGCACCCATTGCTTCACCAGCTTTAGTTGCTGAAGCAATGTTTCCGTGGTATTTAACTTTAGCTTTAGGATCAAGTGTTGAAACACTTGCTAAAGTGTGTCCTGTTGTATCGTCAATTAGTTGTGCATAAAAGTTTGTATGAGATTTGAATACGCAAAGACGTGGTTTAGTAGCAGTACCTGAAATACTTTGACGTTCACGTAAGTGTTTAACTTGACGTGCTTGATTACGAGATAATGTAGCCATAATATTTTTTCCTTTCAACTAAACTACTATTTAGAAGCAGTTTTTCCTTCTTTAATACGAATGTATTCGCCTTTGTATGAAATCCCTTTACCTGAGTATGAGTTAGGACGACGAACATCATGCACAACAGCAGCAAATTGACCAACTTTTTCTTTGTCAATACCACTAATAATGATTTCAAGAGGTTTTGGAACTTCAACTTTAACACCTTCTGGTACACTTAGACTAACTATGTGGCTGTAACCAACAGTTAATTCTAAAGTTTGACCATTAAGTGCAGCTTTGTAACCAACACCTTTGATTGCTAATTCTTTTTTGAAACCTTTTGAAACACCTTCAAGCATGCCTTGAATTAAAGCATTTGTTGTTCCGTGTAATTGTTTTGTGTGTTTTTCTTCATTAGCACGGAGGGTTGTAATTTGATTATTTTCAATTTTAATTGCAATAAGTGGGCTGAATGTTTTTTCTAATTGGCCTAATGAACCTTTAACATTTAAGGTTGTACCATTTAAATTAACTTCAACACCTGCAGGGATATTTAATACTCTATTTCCAACACGAGACATAAAGCACCTCCCTATCAAATGTAGGCAATGATTTCACCACCCACATTTTCCTTACGAGCTTGTTTTTCAGTCATCATTCCTTTTGAAGTTGAAATGATTGCTGTACCATAACCTGATAAAATGGTAGGTAATTTGTCAGCTGAAGTATAAACTTTAAGACCTGGTTTTGAAACACGTTTAAAGTCAACAATTGCACTTTGGTTGTTTTTGTATTTTAATGAAACTTCAAGTACTTTGTCTTTACCTTGGCCTTTTGTAACAAAGTTTGTAATGTAACCTTCATTTTGAAGAATTTCTAGAATTTTAGCTTTTTTATTTGAAAAAGGAATAGCTACAGTTTTGAATTTACGTTGATTAGCGTTTTTGATTCTAACAATCATATCTGAAATTGGATCTGTGATAAACATAATTATCAACTCGCTTTCTTCATGCCAGGAATTTTTCCTTCATGAGCTAAATTACGGAAACAAATACGGCAAATCTTGTATTTTCTTAAAACTGCGTGTGGACGTCCACATAATTCACAACGTGTGTAAGCACGTGTTGAGAATTTAGGATGTTTCTTTTGTTTTGCTTTTAATGAAACTTTAGCCATATTATCTATCTCCTTTAATAAATGGAATGCCGATTAATTCTAATAAACTTCTAGCTTCTTCATCTTTATTTGTGCTTGTGATTAATTGAACATCAAGTCCCTTGATGTGACGAATTTTGTCAAATTCGATTTCTGGGAAAATAATTTCTTCTTTAACACCTAATGAGTAGTTTCCTCTACCATCGAATGCTTTAGGGTTGGCACCACGGAAATCACGAATACGTGGCATTGCAACATTAATTAATTTTTCTAAAAAGTCTCACATACGATCACGACGTAGTGTAACTTTACCACCCATAGGCATGCCTTCACGAAGTTTTCATGAAGCATTTGATTTTTTAGCTCTGGTTTGGAATGGTTTTTGACCTGAAATTTGAGTAAGTTCATTAAGAACTTCTTCGATAGCTTTAGAGTTTGTTACTTCTTTACCTGCTGTCATATTCAAAACAATTTTTTCTAAACGTGGTACTTGCATAACTGATGAATAGTTATATTTTTTCATTAATGCAGGTACAACTGTTTTTGTGTAATAAGCTTTTAAATTCATAATTAAATTTCCTTCTTAGTTTTACGAGCAACTCTAACTTTAGTGTTACCTTTAACTTTAAAACCAACTTTTGAATATTGAGCAGCACCACTTTTGGTAGCTTTTTTAGTAACGATTGCAAGATTTGAAATGTGAATAGGAGCTTCAACGTTCTTGATTGAACCTTCTGTATTACTTTGACTTGGTTTTACGTGTTTAGTTACTACGTTTACATCTTTTACATATGCACGGTTTGTTTTAAAATTTGTTCTAATAATACGACCAATTTTACCTTTATTGCTTCCTGCAATAACAATAACTTCGTCGTTCTTCTTAAGTTTTAATTTCATAATTACCTTCTTTATTTATTATTAAAGAACTTCAGGTGCTAATGAAACGATTTTTAAATAACCACGGTCACGTAATTCACGAGCAACAGGTCCAAACACACTAGTACCTCTTAAAGTACCATCATCTTTAATTAACACAACTGCATTATCGTCGAAACGAATATGTGTACCATTTTCACGGCTAATTCCATAAACACTTCTAACAATAACACCTTTAACTACTTGTCCTTCTTTTACTAAACCATTAGGTAAAGCTTTTTTAACTGAACAGATAATAACGTCACCGATATTAGCTGTTTTCTTTCTACTTCCACCTAAAACTTTGATTACACCAACTTCTTTTGCTCCAGAGTTATCTGCAACATTTAATTTAGAAAGTGCTATAACCATTATTTTTCTCCTTCAACTGCGTGTTGTTTAATTTCAACAAGACGGAAGTGTTTGGTTTTTGAAAGAGGACGTGTTTCCATAATTGTAACGATGTCGTTAACTTTAGCTAAGTTTTTTTCATCATGAACTGCGAAACGTTTAACAACTTTAAAACGTTTTGAGTAAAGACTATGAGCTTTATAACTTTCAACTTCAACGATGATAGTTTTATCACCACGTGCTGAAGCAACACGACCTTGAAGGGTCTTACGTGAACTTCTTTCCATTATTTAGCTCCTCCTTTAGATTCGTTAATTTTTAAAACTGTTAATACTTTAGCAATGTCACGACGTACTTCTCTAATTTTGTGACTGTGATCTAATGTCCCAGTTTTGTTTTGGAAACGATAAATAAATAATTGTGCTTTTAAATCGTCAAGTAATTTACGGAGTTCAGCATTATTTTTAACTTTAATATCTTTATAAAGCATTATTGTTGACCTCCTTGTTCTTTAGCTACTATTCTTCAGCTTACAGGTAGTTTGTGACCACCTAAACGTAAAGCTTCTCTTGCAATTTCTTCGCTAACACCTGATACTTCGAACATCATTGTGTTAATTCTAACATGTGTATATCATTTTTCAGGACTACCTTTACCAGAACCCATACGAACACCGATAGGTTTTGATGTTTTTGAGAAGTGAGGGAAGATTCTAATGAAAACTTGACCTTCACGACCCATATAACGAGTGATAGCAATACGAGCACTTTCTATTTGACGTGCATCGACTCAAGCTGAGCTTACAGCTTGTAAACCAAAGTCACCATAAGCAACTGTATTACCTTTGAAGGCTTTACGTTTGTCATGACGTACTAAAAATGGTTTTCTATATTTAGTTCTTTTTGGTTGAAGCATCTTTTGACCCTCCTTCCAAAATTTCGCCTTTTGAAATTCACACTTTAACGCCGATTACACCATAAGTAGTGTGAGCATTTGCGTGTGCATAATCAACATCTTGTCTTAATGTGTGAAGTCTCATTTCTCCACGTGAATAGCCTTCTGTTCTAGCCATATCAACACCATTAAGACGACCTGAAACTGCAGTTTTAATACCTTTAGCTCCAGCTTTTAATGCATCATTAATAATTAATTTTTGTGCAATACGAAAGCTTTCACGATTTTCAAGTTTAGTTGCAATAGCTTCAGCAGCTAAACGAGCATTAAGTTCAGGATTTTTAAGTAAAACAACTTGTAAATTAATATCAAGATTTTTGTTTTTTAAGTATTTGTGTAAATCAACATTAATTTTGTCGATTGTACTTCCACCTTCACTAACTAACTTATTTGGTGTAGCTGAGTGAATGTAAACTGTTATTTTATTGGCTTGATTTCTTTTGATTTCAACTTGTCCAATTTGGTATTTACGTACTAATTTATCGAAGAATTTGTAAATTTTAGCATCTTCTACTAAGTATTTTCCATAGTTTGCTTTTTCAGCAAATCAAGATGTATTACGCGCTTTTGTAATTCCGTAACGGAATCCATTTGGATTAACTTTTTGTCCCATAATTTACTACTTTCTTTCTTCTAATGTAATTGATAAATGTGATGTACGTTTAAAAATTGAATAAGCTGTTCCTCTTGAGTGAGGTTGGTATCTTTTAAGTGTTGGTCCTTCATTAACTAAGATTTCTTTAACAAATAATTTAGATGCATCCATTCCGTGGTTGTTTGTAGCATTAGCAATTGCTGAGTTAATAAGTTTAATAAATAATTCTGAAGATTTCTTGTTTGTATTTCTTAAAATACCAAGAGCTTCACGCACGTCTTTAGATCTAACTAAGTCAGCAACTAAACGTGCTTTACGTGCACTAACACGTTGAATTTCAACATGAGCTTTAGCTTGTTGAACAACTGGTGTATTTTGTGCCATAACTATTTCTTACCTTTGTCAGCACCGTGACCTGAGAAGGTTCTTGTAGGTACAAATTCACCTAACTTGTGACCAACCATGTCATCTGTTACATAAACATCAATAAATTGCTTGCCATTGTGTACTTGGAATGTTAAACCAATGAAACTTGGAAAGATGGTTGAACGTCTTGATCAAGTTTTAATTGGTTTTTTTGGCGCTTTGCCTTCAACAATAGCATCTACTTTTTTAAGTAAATGTTCGTCTGCAAAAGGTCCTTTTTTAAGACTACGAGCCATTATTTAGCATCCTTTCTTCTTCTTACGATAAGTTTGTTTGAAGATTTCTTAGTTTTTCTTGTTTTAACTCCAAGAGCTTTTTTACCTCAAGGAGTAAGAGGAGCTTTACGACCAACAGGTTGTTTACCTTCACCACCACCATGTGGGTGATCCACAGGGTTCATAACTGAACCACGAACTGTTGGACGAACACCTTTATAACGGTTTCTACCAGCTTTTCCTAAATCAACCAATAAATGTTCTTCGTTACCAACTGAACCGATAGTTGCACGGCAACGAGCTAAGATACGACGCATTTCACCGCTTTTTAGACGTAAAACAACATATTTACCGTCGTCATCTTTACCTAAGATTTGTGCAGATGTACCTGCTGATCTAGCAATAATACCTCCACCACCTGGTTGCATTTCAATGTTGTGTACAATTGTACCTTCTGGAATGCTTGAAAGTGGTAATGCATTACCTACTAAAATGTCAGCGTGTTCACCTGAAATAACGCTTTGGCCTACTTTAATGCCTTCAGGCGCTAAAATATACCTTTTTTCTCCATCAGCATATGCTAATAAACAAATATTAGCTGAACGGTTAGGATCATATTCAATGGTCTTAACTACTGCTGGAATATTGTCCTTATTACGTTTGAAGTCAATTAATCTGTAAAATCTCTTAACACGACCACCCTTGTGACGTACTGTAATCTTACCTTGGTTATTGCGACCAGCACTATATTTTAATAGTGTAAGTAAAGATTTTTCAGGAGCATGTCCACTTAAGTTTTCTGTGTAATGTAGAGATGACATATTACGACGTCCATTCGTAGTTGGCTTGTAATGTTTAATTGCCATTTCTAATTTTCTCCTTGCTTAAAAATAGATGAGGAAATTTTTCTTTTAAGCAAAATTTAATCCTCTAATAATTATTTTGAACTCTTCTTAGCAGCTGCAACTTTTTTAGCAGCAGGTTTTGAAGTAGTTGATTTTGTAGTTTTTTCAGCTTTTGAGCTGTCTGCTTTTTTGCTTTCAGCTGCTTTAGCTTTTTTAGCAGCAAGTTTTTTAGCAATTTCTGCTTCTTTAGCTGCTGCTTGTTTTTTATCTTCAGCTTTTTTAGCTTTAACTTTAGCTTCTTTTTTAGCATCTTGAGCTTCAGCTTCATTTGGATAATAAACGATTTTATCTCCTTCAGCTAAAGTAATCATTGCTTTTTTGTAACGATTTGTAAAACCGTTGTAACGACCTACTCTTTTAGGTTTTTTGTCAACTTTTAAAATGTTAACTTTAACAACTTTAACTTTGAAAATAAATTCAATTGCTTCTTTAATTTGATATTTGTTTGCAGCTCAATCAACTTCTCATGTGTATGTGTTTGAACTTTGTAAAATGTTGGTTTTTTCAGTGAGAACTGGTCTATGTAATACTTGAGTAATTTCCATTATTTAATTCTCCCTTCAAAGTTTTTAAGACCTTCATTTGAAAGAACTAATACATCTGCTCAAACAAGGTTTTCAACACTTACTGAGTTTGGTTTAACACATAAAACATTTTGTAAGTTAGCTGTTGATTTGTAAACAGTTTCATCTGTTGTTACCACTAAAATGTGTTTTAAGTTTTCAATTTTTAATGATTTAAGTTTGTTAACAGCATCTTTAGTTGAGATTTTTGACATTTTAAAGTCATCAATAACAACTGATTTTTTGTTTGAAAGTAATGTAAGAGCTGATAAGAATGCAGCACGACGTACTTTCTTGTTAACTTTTAATGAGTAGTTACGTTCTGGAGTAGGACCAAATGCACGTCCACCACCAACTCAAATAGGTGATCTTGTTGAACCTGCACGAGCACGTCCTGTTCCTTTTTGTCTTCAAGGTTTTTTACCACCACCGCTTACTTCTGAACGATTTTTAACATCATGAGTACCTTGACGTCTTGATGCTCTATCAGCCATAATGGTGTCAAAAATTGCTTGTTCATAAATTTTTGGTGATGCAAATAATTCTTTTGGTAAGGCTTTATTGTCAAAGTGAAGCAAGATAACTTTTGTAAATTTTTCTTGTTCAAGTTTAGCATCATTTAATTTAAGTGAAGCAACTTTTGCTCTTGAAGATGCATAAGTTTTAGCTTTTCTTTTAGCAGCTTCTGCTGCAGCTTTTAATTCATCTAAAGCTGCTTGTGCTTCTTTTTCTGCTTTTGCTTTTGCAGCAGCTTCTTCTTTAGCTAATTTAGCTTTTTGAGCAGCTGTTTTAGCAGCGGTTTTCTTTTTAGCACTTTCTTCTTTAGCTTCTTGAGCTTTTAATTCAGCTTTAGTAGGCTTTTTAGGTTTTAAGTTTTTAGTTAAATCAATTTCAACTGTAACTTTTTTTCTTGAAGTTGTTTTCTTTTCAGCTGTTGCTTTAGGAGCAGCGGATTTTTTAGTTGTTGTAGTAGCTTTTTTAGCAGTAGTTGTTGATTTTTTAGCTGTAGAAGTTGATTTAGCAGTAGTAGTTTTCTTAGCAGTTGCTACTTTTTTAGTTATAGTTTTCTTTTCTTCACTAGCTTTAGTAGTAGCTTTTTTCTTTGTTTCAGCCATTATTTATCTCCTTCTGCTTTTTTACTTTCTTCAGCTGCTGCAGCTGCATCAGCTTCAGCTTTTTGGATAAGAGGTTCTAATTCACTTGAATGCATACCAACTGTAACTTCAATGTTGTATTTTTTAGCTCTTTCAAGCAATTCATTCATTTTAAGAACTTCTTTAATATCAACAAGTTTAATTGCATCATGATTTGGTAAGCCTTTAACAGCTTCACGAATCATAACATATGAATTTTTAGCCCCAGGAATTGATCCTTTAACTAACATAATGTTGTTTGCTACATCAACTTTAACAATTTCAAGATTTTGAACTGTTGTTCTAACAGCTCCTAAGTGACCTGGCATTGTCATACCTTTGAAAACACGGTTACCTGAGATGTCCCCAAGTGATCCTGTTTGTCTAACAGGTTGACTACCACCACCACCACCGTGTGATTTAGGTCCAATGTGTTGGTTTCATCTTTTGATTGTACCGGCAAAACCTTTACCTTTTGATGTACCAGTGATATCTACTAATTCACCAGCTTTAAAAATGTTGGCCTTAATAGTTTGACCTAATTCATAGCCTTGCATGCCACGAATTTCTTTAACGTAGCGCTTAGGTGTTGTTTTAGCTTGAGCAAATTGACCTTTAAGAGGTTTATTGCTTCTGTGTTCTTTCTTTTCACCAACAGCAATTTGGGTTGCAACATACCCATTTTTGTCAGCTGAAAGAACTTTAGTAACAACATTTGGTTGCACTTCTATAACTGTAACTGGAACACTAGCTCCAAGTTCAGTAAAGATTTGTGTCATACCAATCTTCCGTCCTAAGATTCCTTTCATAATTTTTTCTCCTTAAATTTTGTTTGTAATTTAACAACCGTCGCTTGTTGTCTAGCACAAGTCGGATTTTGTTATTTTGTTGAGATTTGAACTTCAACACCTGCTGGAAGTTCTAATCTCTTAATTTGGTCAAGAGCTTTATCGTTTGGGTTTAATAAAACGATCAAACGTTGAAAAGTTCTTGATTCAAATTGTTCACGAGATTTTTTGTTAACGTGAACTGATCTTAAGATAGTCACAACTTCTCTTTTTGTTGGAAGAGGAACTGGACCACTTACTTTAACTGAGTTTTTGGTAGCAACTTCAACAATTTTCTTTGCTCCAAAATCAACTAAAGCATGATCGAAACTTTTAACCTTGATATTGATTTTTTTCATAGTTGTCTCCTTTGTTCATATACGAACTTTACTCCACATAAATTACTTGAAATGTCCTCAACAACTCTTCTTGGCATTTCAACAACCTTATGCTTCTATGTAATAGTTGCTTTTCAATTATAAATGAAGACTTAAAATTTCAAAGAAAAATATTAAATATTTTATTATTAAGTATAAATGCTAAGTAAATAAAATCAGCTAATAAATTAAAAAGTTAATTTTTACTTATTTTTTAGCTTATAAACTCACTTTTTGGTTTTTTGTGCAAATTTTAAAAATGTTGATTTTTTAGCCAAAACATCAATAAGTTATTAAGTAAAAACTTGAAACACCGAAACAAGTTGTTAAAATTAATTTAAACTACTTTTTAATGCTTTTTGTTATTAAAAAAGTGATTTTTTAAACTATACCACATCTCTATAAAATATAAAGATAAAAATAATTAATATATGTATATAAAGAAAATTTGAATTTTTTTGTTTAGGTGTATAAAAAACAGATATATAATGTAGCTGAAGATTAATCACATTCTATATATAATATTTTGAAAAAAATATTTTTGTTTTATAACAATCTATCATTCTTAAAAATAATTAAAAAAATTAATAATAAAAAGGAAAATAACTATTATGAAAAAGAAATTATTCTTTAATTTTTCATTGTTATCTTCTGTAGCTCTACCACTTACAACTATTGCAGCTAGTTGTAATCAAAACTCAGATCAAAATATACCTAACATTACAGTTAGCGCTAGTGAAAAAGGTTTAGGCTACTATCCAAAAACAATACAAAGATTAATTAAAGGCAAAAAAAATAAAGAATTAATTGATACTATTAATCAATTTATAAATAATAAAGAACAAATTAAATATGAAAATAATAAAGAATATTCTTTAAATGAAAACGAAAGTACATTTGGTTACAATGAATTAAAAAAAGAGTATAAATGCATTATTGCATTAAATATTAAAGACAATGACAAAACTAAAGTTAAAAAATTTTCTATTAACAAAATTGGTCACAATAATGTTTATCATGATGATATACACAAATTTGATAGACTTGAGAAAGTTTACTTTTGAGATGGTCAATTAGTAATTGATGGTTACATTAAAGATTTAGAAGTTATTAAAAAAGTCAGTTCAGGTGAAGTAGCTCACGAAATTAAAAACAAAAGTTTTGAAGAACAAATTGAAATCATTAAAAAATTATGTAAAGTTAATATTGAATTTCATCCCGATTCTGCTTATGACTACAAAAGTGATGAAGCAACACATTCTCACCCTGATACTTTACACTATGTTTTTGTACGTATTAAAAAAGATAACCCTGCTGATAGTAGCATGGTTTCTCGTGATATCCAAGGCTTTGAGCCTAATGCTAAAGTCGATCCAAAAAAATGACTAGGAGTTTATGCTTCTGGAGGTGTTCAAGTTAAAGAAGGTGAAATTGATCACGTTGTTGGACATAATGCGGCAGGTGACATTGCTGATAAAAAAATAGGTTCATTTGTATTTCGTCCAAGCGTTAAAGATGAAGGCAAAAAAATGACATCAAAAGACTTTTTGGATTTACTAGATAAGGTTGATAAAGAACATAATCATGAACCAAAAGACATAATTAATTTTTTAAAACAATATGTAAATATTGATGGTACTTATGATGCAAATGATAAATATGAATATGCTTTTAACGTAGAAAAAACTCATACTCATGGTTCAGGCCAGTTGCACTTATATACTTACTACCGTGAAAAAGGTAAAAACCAAAAATGAGTTGAAAAATCAATCAATATTGTTGGCTGAGGAAGCAACTTTGAAATGGGCGGATTAAAATTCAATAATGCTGGTATTAAAGACGCAAAACAAGCAAATGAAGATCCAGGATTTTACACAAGTCCACATCAAATACTTAGAGAAATGGAAACCAAATCTACATTTGATGAACAACTAGCAGTATTGAAAAAATATTGTGAAGTTAAAGACGAATCAGATAAAAATAAATATGACTACAAATTCAATTTTAAAGAATCATGAAACAATATTCAATATCGTCATGACTTTGAAAAATTATCAATTGTCAAAGAAAAGACAAAAGCAAATGATGCAGCTAATACACTTACAATAACATTGAAAATTGAAAATATATTTACTAAAGAAACTAAAGAATATCCAATTACAATTACAGGTTTCTGAAATGACGGAAAAGAGTATAAAAAAGGCTTTTTCGACAATAAAGTACTATTAAAAGACTATATTAATGAAGATGAATATGATTTCGATTTAAAAGATAAATTTTTAAGTGGATTATTTAGTTTCGATAAAATTCAAGACCAACTTAAATTCCTAAAAGATAGAAAAATAATTAATAAAGCAAAAGATTTTGATGGGGCTAATGAAGAATTTACAACATTTGCTTCAAAAGATTACAAATACTACTTTGATAAAGAAAAAACAAACAATTTAGAAAAGGTCAACAAAGATAAAAGAACGTTAGAATTAGTATTAGTTAGAGAAAATTTAAAAAATCCTTCAGATAAAAAAGAAGAGATTATTAAAATACATCAATTTTTAAAAGATTCCAAAGATGAAGTAGGTGCATTAATAAGTGCTTCAGGACATATTAAATATAAAAAATATTTCACTCCAACTAAAGACGCCAAAAACAAAAAAGCAAGCGATTTATATAAAGAACTTGAAAAGAACAATTTTGAATTCACAACTAATTTAACAAGTTTAATGTATAAATGAGGTATCTATTTTATATCAAATAATATTAAAAATAGAGATTATGATGACAGAAGTCACTTACATTTAGTTTTAGAAGTAACTGAAAAAGCAACAAAAAAAACCAATGTTATGAGACTTGATTTAAAAAACTTTGGAGCATTTTCTAAAGTTGGCGATTTTTTAATAAAAAATACACCTTCAGAAACGGATATTGTTAATGTTCGTGCTCAAGACATTATAAATAAAGCTAAAAGTATTTGAGAAGATACTGCAAATAAAAATAAAACTTCAGGTCAAAAAATGAAACTTTGAGTAGATTACCTAAAAAATAATAAATTGGGTTTCCCAGGATATGATAATACTGTTATTAATGAAGATTTAGAATACACAATTGATTTCAATAAAGCTGTTGTATATAAAAGTGGAAAATTAGAATTGAACATTAAAGTAAAGAACACTAAAACAAATGAAGAAGAAAATGTTTATATTCTATTTTGCAAATTAGCCTAAAATCCTTTTCTATCGTTTTTCTAATAATTAATGAGTCATAATTGACTCATTTTTTATATCTAAAAAGCAGCAAAAACTGCTACTTTTTGGATTAGATAAATTATTGTCAATCGTCACATTTGTAGGTTTCATCATGGTTGTAATTTTGGTTATTTGATTGTGAATTGTTACCTGTTGTTTGATTTTGATCTATATTAGATTTGCGTTGTTGGTTCACATTTTGATCTTGTGTTTTTCTATTTTTAAGATATTTTTCAAAATAGCATGAAACCACCAATGACTAATAAAATAATTCCTGAAACTAATGTTCCTATTCTTTGAGTACTATCAAATGTTTTTCATTTAACTCAAGCAGCAATAATTCCTACAAAACCACAAATTACACACAAACCACCAAAATTCAAAAACCAAATTTATCAAAAAAATGATTTTAGAGCACTACAGGTTGAATTTTCAACTTCTTGTTTAAGAGCATTTGAAAATGCATCTTTGAAACTTTTTGCTATTATTTGCCCACTTTTCTCTTCTAATTTCAATTGTCATATAATAAAATTATTTAAATATAACCATATTTATTTCACATAAAAATACTCTAATGAGTATAAATGTATTATTATTTATATGAAAAAACATTAAACTATGTTATTTTTGGTAAAAAAGCCTCAATTTTCACAATTTTTTTCAATTTTTTTAGTAATTTAAGCATAAAAGGCAAGTTTTAATTGAAAGGATAAAATCAACATGAAAAGCAAAAATATTGAAATTAAAAATAAAGACAAAGAATTAAAAGAAGCTTTAGAAGAAATTACTAAAAAATTTGGATCTGAAGCAATTATGCTTTTTGGACAAAATGAAATTGAAGAAAAAATTGAAACTTTTCCAAGCGGTTCATATCTTTTAGATAATGCACTTGGTATTGGCGGTTATCCAAAAGGAAGAATTATTGAAATTTATGGACCTGAAAGCAGTGGTAAAACCACACTTTGTTTACATGCAATTGCCGAAATTCAAAAACAGGGAGGAATAGCAGCTTATATTGATGCTGAGCACTCACTTGATCCAAATTATGCTCGCAATTTAGGAATTAATCTTGATAACTTAATACTTTCTCAACCTGATAGTGGAGAACAAGCTTTACAAATAGTCGATATATTAGCTAAAAGCGGTTCTATTGATTTAATTGTTGTTGATAGTGTTGCTGCATTAGTACCTGAAGTAGAATTAAATGGAACAATGAGCGATTCTTCTGTCGGAGCTTTAGCTCGTTTAATGGCTAAAGGTTTAAGAAAAATAACTGGTAATTTAAACAAAAATAAAACAGCAATTATTTTTGTAAACCAAATTAGAGAAAAAATTGGTGTTTTATTTGGCAATCCAGAAACAACAACTGGAGGCCGAGCAATTAAATTCTTCTCATCAGTTAGAATTGAAGTAAGAAAAACCACATCAATTGTAGAAGGAAAAGACATAACAGGAAATGAAGTTAAAATCAAAATAGTTAAAAACAAATTAGCTGCTCCTTATAAATCATTTACCACTGAAATTATTTTTTCTAAAGGAATCGACAAGTTAAATGAGTTAATTAATTTAGCTACAGAAGCAAATGTTATTGAGAAAAAAGGTGCTTGATTCGCATATCAAGAAAAGAACATTGCGCAGGGTAAAAAAGCGTTAAAAGATATACTTTTAGAAAATCCAGAATTCAAAAAAGAAATCGAAAATCAAATTTTTGGTAACGAAGTCAAAAATGTTTCTAAATAACTTTAAGCCCTATTTTATAGGGTTTTTAGTTTAAATATAAATTATGGAATTTAAAAAAATTTATTGGCGAAGATAACAAAATTAATTTTTGGGTTGAAAAATTTTCTATGTAACATATAAATATTAATAACGAAATCATATGTAAAAAACAAAAGCAAAAAAATAAATAATTATTATTGCAACATCTATTGCATGATAATATGCATTCTTAGTCCCATCAAATCATTCATGGATACAAGCATCTATTTGTAATTCATCACTAAAATGCATGTTTTCTTTTCTGCATAATCTAACTCTTAGTCTTACTTCGCTAAACAAGCCTAAAAAGTTTTGATAATTGTTTCTAACATCTTCTATTTGGACTTTTAACAATTCAGCAATTTTAATCATTTTTTTACTTTTCTTCTCGTTTTCTTATAGTTAAAACTCCTAAGCTTGTTAATCTGTTAACTAAAGTTTGATAACTAATTTATTTTTCATAGCCTTCATGAGAATAATCTCCAACTTTTATTTCTTTTCAAAAAGATTTGATTGTTGCATCATTAACATTTTTGATTCCGTTAGTTCTATTTTTAGAAACAATTTGAATAGCATTGGTTTTTCTATTTAAAAAATCTCTAATATGGCTTTTGTATCTTTGAATAGATATAATTGATAAACCAACTTTTTTAGCAATTTGTCAATTACTCAAATTCATTCAAGGTGCAATTAATTGATAGTTTTTTGTTTGCTTTTTCCAATTTTGCTGGATTAGAACAAAAACTAAATACTAAATCAATAAATACATCCTATTTTACAATTATAAATGTCAAATTGTTTTGTATTGAAGGTATGACAAACTATTTTAGAATTTATATCCCACTTGTTATTTCTTATTACTTTTATATTTTAATAATATAATAATTTTAATAATATATTTTGATTTTTATCAGTAGAATGAGGTCATTAATATGACAAAAGAAAACAAAACTTTAAATATTCTTTTTATTGGGGATATTTTTTCTGAAGCAGGAATTGAAATAGTTGAAAAACATTTACCTTCTTTAATAAAAGAAAATAAAATCGATGTTGTTATAGCGCAAAGTGAAAATGTTTCAGGAAGAAAAGGTTTAGTTAAAAAAGATTATTTAAGACTTAAAAAAGCAGGTGTTAATATTTTTACAATTGGAAACCATGTTTGAGCTAAAGATGGAATTTTTTCAATAATCAATAATCCTGAATTAGTTAGACCTGCTAATATAGATGAAGGATATCCAGGAAATGGATCAATAGTTTATAAATGTAATAATGGAACTACTTTAAGAGTTACTTCATTAATGGGAATTACTTTTAATAAACTATTAGCCCCTTGAACTCAAGAATATGCCAATAACTTTTTTGATACTATAGATAACATCATTGAATATGGAGAAAAAACTGATTTTCACTTTGTTGACTTTCACGCTGAAACAACAAGCGAAAAATATGTTTTAGGTCTTTATTTAGATGGCAAAATTGATGGTATTTGTGGCACTCACACACATGTGCAAACTAATGATGCACATGTGCTACCTAAGGGGACTTGTTATTTAACAGATGCTGGAATGAGTGGGCCATTTGATTGTGCTATTGGAGCAAACTTTGATGAAGTTTATCAAAAAATGAGATATAACAAGAATGTTAAATTCATTCCTTCAAAAAACAAAAGCCAATTTAATGGTGCTATATTAAAACTAAATAGTGACAAAAGCCAAAACAAAATTATTCCAATTAACATAAGACAAGAATAAAAAATGATATAACTAGTTCAAGCTTTACATAGGTAAAGCTGCAAGTTAGTCAAAGAGCTTGGCTATTAGACTAATATATGTGTTACTAATGAACAAGACAAATGTGCTTGAATCAATGGACCAACATTGAGGGTGTAGACTCTTGCATTGTATAGTAAGTGATTTTAAGTGGTATTCTTTACCGCTATTTTTTTACAATTTATTATAAAATTTGCAAAGATAAAAAAGAAATAAAAATTATGAAATATCCAAAGTCTTTTGTTGATTTTAGTAATATTGATGTTGATTTAGAAGCTAGTAATGAACTCTTTTTAATGGGTCCACTTGTTTTTTTATTTTATTAATCCTTTAATAAAGCTATGGCTTTTATTTTGCATAATGTGATTTTTCACCAACTTTGACATTAAAACAAATTTTGTATTCTCCTGATTTTTCTATTGTTTTATAATCACTTAAAAAAAATCTATTTGAATATTTTTCTAAAGCTGTTTGTAATAAAATTATATTAAAAATGCTACTTTTACTTAAAATTAAAAAGTATTCATTAATTTGATCGCTTGATTTTTTAATTTTTCTAAAGCTATTTCTTTAGTTACTTTTGAAGTATCTCACGTCCCTTTCCTCCGAGTCTCCTTTTTTCTTAGTGCCACAACCTTCACTAATTGTTGGTAATAAAATTGTTGAAATTGTAGGTGTTAAAACTGAGAATTTCAATCAAAAATTGTGCTTTTTATTTTTATTTTTCGTTGTACTTTTTTCTATTTTTTTACTTTAAAAATAAATATTATTTTTTTAAAACATTATGTTATATAACGCAAAAGAAAATAAAAAAATATCAGAATTTTAACTAATTAATTCTGATAATTTCTAGTTGCATTTTGTCTTAAACAACTGTTTTACAGTATTTTTTGATTTTATTTAGCTTTTAATTCATTTAACATTTTGTTGTCAAAAGCTTTGACAAATTCTGTAATTAAATCAATTGTTGCTTGAATATCTACTAATGAACAAACGCCTATTGGTGAGTGTAAATAACGTTGAGGTAAACTTAGAGTAATTGTAGGAACCCCACCTTTACCATATTGTAAACTAGCTCCATCAGTACCGCCACCTTCGGCTATATATTTATAAGCTGGAATTTTGTGTTTTTCAGCAATTTTTACTAACATGTTAACTAATTTAGGATCTGTAATAACAGCACCATCTTGTATTAAAAGAGCTACACCTTTACCTAAAACTGGAGTTCCTTTAATACATCCTGTCGTATCATGACTTGCTCCTGTATCAATTGCAAAAGCAACATCTGGTTCAATAATTGAAACTGAAGTTTTAGCACCTCTTGTTCCAATTTCTTCTTGTACTGTACCAACAATATATGTTTCATTCGGTAAATCTAAATCTTTAATATTGTTTGCTAAAAAGTCAATAACTGTAACACCAGCTCTATTATCCATTGCTTTACCAGCAATTAAATTATTAGGCATATCAATTGCTTCACCTGACATATAAATTCTGTCGCCTACTTCAATACCTCATTTTTCAGCTTCTTCTTTTGAATTGAATCCGCAGTCAACAAACATTTCTTTTGTTGTAGGAACTTTAGCACGTTTTTCAGCTTCCATAATATGAATTGATGTATGACCAAAAATACCAAAAATTTCTTTATTGTCTCTATTTGTTACCACACGACATTTTGTCCCAATAACAGCAGCTGGTCAAACACCTCCAACAACTGAAACTAATAAGTTACCATTTGGTTCGATTGATCTAACTAAATAACCAACTTCATCCATGTGTGCAGCAATCATAATTTTTGGTGCATTTTTAACTTTTGTTTTATTTTTAATAATTAAAGAACCAAAACCATCTCTTGAATATTCAAAATTTTTTGAAGCTGTATTCTTCTTTAAAGCTTCAACAACAGGTTCTTCATATCTACTCATAGCTTCAATAGCCATGTATTCTTTTAATCTTTTTGCTAATTTATCATATTTAGCCATTTTTAAACCTCCTTAAAAAATTACTATATGAATTATATAACTAATTTATTTTTGCTAGGTCTATTTTAAGGCGGTAAATTTTTAATTATTTTTTGCATCTTATTTATAGGTCTTTAAGAAAATTAGTTTTAAACTAAAACAGCAAATTTTTATATTTTAGTATCATTTTTAAAATCTTTAATAATTTTTATACATTAAACACAAGCATGAATGCTTGTCGCATTTTTCTTTTTTATAAATTTTTCTAAATATTAGTATACTTATTTCACTTCGGATCGCTAATAATACATAGTATTATAATTTATATACCAAAACGAGAATTGTGAAAAAGAGAAACAAACTAAAGGCTTTAACTATTTTATCAACTGCTTTTGCAACAAGTATTGTTGCTTTTGCTGCTTCATGTAATAATGAAGATAAACAAGTTTATTTTACTTTAAATCATAATTACAACCAAAAACAGCGAAACATATAACATTTTGTCAAATAACTATCGATACTATAATGATTTTGGAATGAAATTAGCTAAAGAAGAAAATTAAGTTTAATATTAAACTAAAGATGCAAACTTTGCATAAAAAAATTAACATCTTTTTATGCAAATTATTAATCACTTTTTTCTAAAATGCCTATAAAACTGAGATTTTAGGCAAAATAGACTATTAATTTTTTACTTTTAGAGTCAATTTTTTGCTTGCAAAAATTATTCTTATTTTATGTAGCAAAAACTATATTATTTTGTTATAATAAATAATATTTATTATTTAAATGGGAGGGAGGTTATTATGAAAAAAACTGTTAAAAATAAGAAAAACAATGAAGCTTTAATAATAAAAAATAGATTAGTTGAAGCTGCAAATATGAATCAAAATGAAATTTATGAAAAATATAATTCTTCAAATTTAGGCATTACAAAAAATGAAATAATAGAAGAAAACAAAGAAAATTATGGAGTTAATAAACTCAGCAAAAAGAAAAGAGGCGGAGTATTAAAAAGAATATGAGATAGCTTCTTCAATCCTTTTTCATTGATCTTGATTTTACTTTCAATAGTTTCATTAATTACTGACATAATACTTCCTTTAACTAAAGGTGAAAAATCCGAACCTTGAACTGTGTCCATTATTTTAACAATGGTTATAATTAGTGGAATCTTACACTTTGTTGAAGAAACGCGGAGTAGTGCTAGTGCTGAAAAATTAGTCAAAATGATTCAAACAACTACTAGAGTAGAACGAAATGGTGTCGCTTATGAAATACCACTTGAAGAAGTAGTTGTAGGCGATATTGTTGTTTTAGCTGCGGGTGATATTATTCCTGCTGATGTTAGAATTCTTAGTGCAACAGACCTTTTTGTTTCACAATCTCAATTGACAGGCGAAAGTGATTCAATTGAAAAGTTTCCTAATTTAGTTCCAAATTATGATTATGATAATGTAACTGATTATCACAACTTAGCTTTTATGGGTTCTAACGTTATTTCAGGCTCAGCTAAAGCTATGGTTGTAGTTACTGGTGATCAAACTTATATTGGTCAAGTAGCTCAAAAAATTAATGAAAAAGCTCCTAAAACCGATTTTGAAAAAGGAATCAAATCAATTAGTTGATTATTAATTAAAATCATGTTAGTAGCTGTACCTCTTGTTTTAATAATCACAGGAACAAGAGCTTCAATTAAAGGAGAACATGAAAAATGGGTTGATGCTTTATTATTTGCAATTTCAGTTGCAATTGGCTTAACTCCTGAAATGTTGCCGATGATAATTACAAGTACTCTAGCTAAAGGTGCTATGAGTATGTCTAAAAAACAAACAATTGTTAAAAGTTTAAACTCAATTCAAAACTTTGGTGCTATGGATATCTTCTGTACAGATAAAACAGGTACCTTAACCTTAGATCAAGTTGTGCTCGAAAGACACTTGGATGTAAGAGGCTTAGAAAATATTAAAGTTTTAAAATATGGATTCTTGAATAGTTATTATCAAACAGGTCTTAAAAACCTTTTAGATATATCTATTATTAATAAGACAGATGAATTAAGTGATGTTGAAATGGAACTAAGATCACTTGAAGAAATCTATAAAAAAGTTGATGAAATACCTTTTGACTTTATTCGTAAAAGAATGTCAGTCGTGGTTAAAAGCATTAAAAGCGATAAAACACAACTTATAACTAAAGGTGCAGTTGAAGAAATTTTATCAATTTGCTCAAAATTAGAATACAAAGGTGAAGTAGTCGACTTAGATGAAAAAATGATTCAAAAAGTTTTAAAACAAGTTGACAAACTAAATGATGAAGGTATGCGGGTAATAGCAATGGCTCGTAAAAGTAACCCTAGTCAAGTTGGTAAATTTGGTGTAGCAGATGAAAAGGATATGATTCTAATTGGCTATCTAGCTTTCTTAGATCCACCAAAAGAATCAACTAAAAGTGCAGTTGAAAACCTTCACAACTTAGGTGTCGAAGTAAAAATCTTAACTGGTGATAATGCCAGAGTTACTAGAGCAATTTGTGCTCAAGTTGGAATTCCTACAGATAAAATTATGCTTGGTAAAGACTTAATGGAATTAAGCGATGAAGAATTGCAAAAAGTTGCTAATGAATACAATATTTATGCTAAATTAAGTCCTGACCAAAAAGCTCGTGTAATAACAGCACTTAGAGCTAATGGCCATGTTGTAGGTTATATGGGTGATGGAATTAATGATGCTCCTGCTATGAAAGTCGCTGATGTTTCTATTTCAGTTGATACTGCAGTCGATATTGCTAAAGAAAGTGCAAACATTATTCTTTTAGAAAAAGACTTAAATGTTTTAGCAACTGGAATTACTGAAGGCCGCAAGACATATGCTAATATGAATAAATATGTCAAAATGACAGTAAGTAGTAACTTTGGAAATATCATTAGCATGATATTAGCTTCGATTCTTATTCCTTTTGTGCCTTTAATGGCAATTCAAGTTTTATTTTTAAACTTAATTTATGATTTTGCTTGTGGAACAATTCCTTGAGATAAAGTCGATAAAGAACTAATTACTAAACCACGGAAGTGAAATGCTAAAAGCATATTGCGCTTTATGCTTTGGTTTGGACCAGTTTCTTCTATTGTTGATATACTTTCTTTTATTCTTCTTTATTATGTTTTCATTCCTTATGCCCATCCTTCTGTAGCCAAAGATTCAGAACAATTCAAGATGCTATTTTGAACAGGTTGATTCATAATTTCAATGTGAACTCAAAGTTTAGTAATTCACTTCATTAGAACTGAAAAAGCTGCATTTATTCAATCAAAGCCAGCCTTTATATTATTATTCTTTTCAATATTTGGAAGTGTATTAATTACTATTACTCCTTATATTCCTGGCCTTAATACAGCTTTAAAAGTAACACAACTTAATCCTTGATTCTATGGCTTTTTAGTAGGTTCAATTGCTTTATATATTTCACTTGTTTTAATTGTTAAAAAAATCTACATCATAAAATACAATGAGTTACTATAATTTACTAAAACATCTATAAAACAATAGAATTTAAAAAACGGTTACCCGTTTTTTATTTTTGCTTTTAGTTAACTCCAGTAGTAACAAATAATTTTTGAAGAGGTTTACATGGTTTTATAGAAAGCGTTAATAAATAATGCCTTCGCCAACTCCAACTCAATATAAATGAATAACATATCTATTAGAAACATTTGTATGTTTTTCAAAATTAATGGTTAATCATTCTCTGTTATCTTCAAAGAATTGTAGAAATTCTTCTGATGTTCATCTTTGATAAGTTAGTGTAACTAATCTTGAACTAGCAACTTTGTAATTGGATGTTTTTTTCTTCAATATCAAAAACAATGTAATTGTCTTTTTCTAATCCAACTTCCATTAATGGAGTAAGATAATCCTTATAATCAGAATCTGAAAAAACATTTATATTTGCATCTCATTCATTATGATTTGGAACAGGAAAATTGAAAAATATTTTTTTAAAAAATAAGTCCCTCAAGACTTATCAAAAATTAATTTTATGTTTATTAATTGTGTCTGAATATTCTTTAAAATTAGGATAATAAAATTCACCCAATTTATAAAATTCTTCAGAATGATCCATATGAAAGTAGTGGCATAATTCATGAACTATCACATAGTCAATATATTTAGGATCAAGTGCCACTAAAAACAAACTATAACTTAGCTTACTAAAAACATTATATTTTTTGTGGTGTGTACCTCAAGCACTTTTTTTCATTCTAACATAAAAATAAGGGTTATTAATTTGCATTAAAGCACTATAAACAAGCTGTTTTTTAGAAATATAGTCTTCTAAAAATTTAGTTAAAGTTTTTTCGAAAACCTTTAATCTTTTATTTTGATCACTGGCACATTTTATTGAGCATCTAATAGTTAAAATATTGTTTTCTTTATCATAAACCCCATCACTAGTATCGCGATAATGAAAGCTTCATTTTAATAATGTTTCTTTGCCAAAAAAACTAATGTAGTGTTCTTCAACTCCGCTAGTTAAATTTAAAACTATAGCATTATTTAATCTTTTATTTGCTTTTTTAAAAGCTTTAATAAGTAGTCTATCTAAATTATGAAAATTATAATCAGCCTCACTAATATGTCTAAAACTAGTGACCTCAAATACTTTTTGGTCACTATTAAATCTAACGATTATATTTTCACAACGGACATTAACTTTTGAATAAACTGGATAACTAACATCATCAATTATGATGTGTTTAATTAAAACTAAATTATTTTTTCTTTGAGTCATTTTTGTTTAAGATCTTTCCAAAACGTTTAATGTCTTTAAGTGAAGCACTTCTATTTGAACTCACTGATTCATTGATTAACATTAATTCATCTTTATTTAAGTTTCGATCTTCACTCATATCTTTAGCCACATCTTCAAGTAAGTTAGCTACTTGATCTTCAGTTGTTACTAAAGCTCATGATTCTAAGTTAGCTATATCAAAAGTAGTATTTGAAGGCAAGATTACCAATGAAAATACTGGGAAGTTAAAGCCACACATTTTGTAAAAGTGTTGAATGTGTTTAAAGTTTTGAATAAGCGGATTTTTAAATTTCAAAACTTTATTGCTTTTGCTTCTTAACTCTAATTCAGCATTAGTAGCTTCACCACTAAGATGGCCAACATAATATTTAACTTCAATAATAAAAACTGCCTTATTACTAATTAAAATACCATCAAGTTCAAATATTTGACTTTGTCCATATTTAAATAAACCACCATTTAAGTATTTGTAGTTTTTATCTTTAGCAATAGTTAGAAGTTTATTTCTAATTTTTGCTTCAAAACGAAAACCAATTGAATTATTGCGTTTGCTTTTAATAAGTTTGATAATTAAAAAAGTTAACACTAGCACAATAATTAATAGTGTTAAAGGAATTCCAACTGATAGTCCAATTATCATTTCTCTACTTGGTTTAATCATATTTATAATTATATTTAAAATTAGAATTTGTAAAGCAAATTAATAATATAATTAAGAAAAGTGAGTAAGGAGGCATTTATGAGTGCAAAGAAAATTATCTCAATTTTAGGTTCAGTAAATAAGAATAGTTTAAGTAATAAAATCAACAATATAGTAAGCAGTAAATTGTTTAAAAAATATCCTAATAGTGAAATCACTTTATTAGATTTAGCAAATTCGCCTTTTAGTGAATTAATGCTTAATGGTCATGACACAACAGCTTTTTGAGAAGATAGTGAAGCAAAAAGCTGAATAACAAAATTAAAGGAAGCAGATTATGTTGTAATTAATAGTCCAATGATTAATTTTAATTACAGTGCTTTAGTTAAAAACTTCATTGATGCAATTTGTATTGCTGATCATTCATTTAGTTATAAATATAGTAAAAAGAATGGATCAGTTGGTTTATTAAACAAAATGAAAGTTATTATTATTGGTACTCAAGGAGCTCCAAAAGGTTGATATACTTTTGGAGATCATGTTAAAATGCTTGAAGGAACATTTAACTTTTTAGGAGCTAAAGAAGTTAAGACTTTATTAGTAGCTGGCACTAAAGTGACACCTCTTAAGGACATGAGTCAAGAAGAAATAATTAAGCAATATCAAAAAGAAATTGATGCTTTAATTTAACTATAAAACAGAGCAATTGGTATAAATTGCTTTTTTATTTGCAATAAAAAATTTCTCGTTTTCATGAGAGAAATTTTTAATCTTGTTTTTTTAAAATAACTTCATTGATAACAAAGAATTTATAAACTTTTTCTAGAATTGCATTAACCATTTTGTAAAAGTTTTTTTCATCTTCAAAATAATATTTAGTTATTTCAACTGATTCATTAATTATTATTTTAGGTTCAATTGACATAAGTTCATGAGCACCATTAATTAAAATGGCTCTAATTAATGGACTAATTCTTTCTCAGCTTCAACCTTCTTTTAAAAATTGACTGACTGCTTTTTTATAAAAATCATAGTTTTTTGCTATCTTTTCAAGATGCGAAAATTGTTCATTACTTAAGTTACTTTCTGAATCAAATAATTCATTGACATTGATTTTTTGATCCATTAATTCATAACGGTAAAGAACATTAATTATACCTAAACGAAATTGACGTCTGTTTTGCATGTTTTTATTTTATAACAAAAGTTATAATTGCAAAATATTTAAATAAAAAGAAGCAACAAGTGCTTCACTAAAATTATTCTAATTCTTCAATCGAAACATTGAATTCACCAATTAAATCATCTTTAGGGCCAAATAATTCATATTTGAATTTTTTAATATTGCCTTCAATAACAACTTCATGTAAAAATGGTTTTAAAAAGAATTGTGTGCCATCTACTTTAACAAAAGAGTTAGGAGCTTTTTGACCTTTTAACATTGTCAACGTTGACATTCCTGTAAAAATATTTACTCTTTCACTATTGATTTCTATACGGTTAGATTCACCAATTGAAGGCTCTTTAAAATGATATGTATGATATTGTAATTGCTTTGGATCTTCATCATCTAAAAATAAATCATATTCTGCATCAGCTTCAAAAGAAACTATTTTAGGTTCTTGGTTTTTAATTTTATGATCTTGAATTGAAGTAAATCTTATTTTCATTTTGCTAACCTTTCTTTAATTAAGTCTTCGCCATATAAATAAATTGCTTTAGCTAATTCAGGACCATGTTCAATGTAAGTGGTTGCTAGACGTATTGGCATAAATAGATTTTTGCCTTTTTTACCTGTAATTTCTTTTGTTTGGTCAATTGCAGCTTGAATATTTTCAATAGTAAAAGTGTTTTTATTTAATAAATCTTTAAAAGTTAAAATTACATCATGATTTTCAGCTTCGACAACTGGTTTTTTATTTGTTGGTTTTAAATATGGCTGCATATCTTCGATTAATTGCGAAGTTGTAAATACACTTTCTTTAAATGTATCAACAAATAATTTGTTTCATTTTGTTGCTTTTAAATTAAGTTCTTTAAGTAACTTAGAAGTTGGTTCTTCTTTTATATATTGTTTAGAAAATCATTGCATCTTTTTGATGTCAAATTTTGAAGGGCTTTTGCTTAGTCTTGCTGGGTCAAATTTTTCAATAATTTCTGCTTTTTTCATAACTTCAACTGTATCTGGTGAACTTCAACCAAGCAAACTTAAAAAGTTAAATACACCTTCAGGATGGTAGCCTTCATTACGATAATCTTCAATAAATTGTTTTAAACTAGTATCACGTTTTGAAAGTTTTTTACCTTCCATATTAGTAATAACAGTTAAGTGGCCAAAGTAAGGAGCTTCTCAATTTAAGGCTTGATAAATTGCTAATTGTTTTGGAGTATTAGTGATGTGTTCTTCGCCACGTAAAACATGGCTTATTTTCATATCGTGGTCATCAACTACAACTGCAAAGTTGTATGTTGGATAGCCATCACTCTTTTGAATAACTCAGTCTCCGATATCATTTGAATTAAAGCTGATGTTACCTCTAACTAAGTCTTCTCATTCATAAGTTACATCCTTAGGCATACTTAAACGAATTGAGTAAGCTTTAGCTTTTTCTCTTTTGGTTTTTTCAGCTTCGCTAATTTTTAATCAATTACGATCATATCTAAATGAAGCAATACCTTTTTTGTTTGATTCTTCATGTTGAGCTTCTAATTCTTCGCTAGTGTCATAAGCTTTATAAGCTAAACCTCTTTTAACTAATTCATCAGCAATTTCTTGGTATCTATCTAATTTTTCGCTTTGGCGATATTTACCACAAGCCTTATTTGGTTTAAGTGGGCTTTCATCTGGAACTATTCCAAGTCAAGCTAAGTTTTCTAATTGACTTCTTTCACCATCAGCTACATTTCTTTTAACATCAGTGTCTTCTAATCTGAAAACAAAATCACCTTTAAAGTGCTTAGCATATAAATAGCAAAATAATGCTGTTCTAGCACCTCCAATGTGTAAGTAACCTGTTGGACTTGGCGCATATCTGGTTCTTATTTTTTTCATTCTAACCTCTTATTTATGACTTAATTTGTATATTAATTAATATTATATAGAAAATGTTTATTTTAGTATCTAAAAGGCAAAAAAAGAAAATTAAAAAAGATATCAAAGTAGAGTGAACCCAAATTCCGAACTTTTTGGAAAGGGGTTCACTCTAAATTTCTAATTAATTTTAATTTCATTGATTTCTCCTTATAAATATAATTTGCTAAATTATTCAATAATTTTAATAAAAAAGCCTACTAATTAATAGTAAGCTTCGATAAATTTTAAGCTTTCGAATTAGAATTTAAAATAGATTTGCAGTCGACAAAACATTGCTAATTACATCTAATGATTCATTCAAATTATCAAAAGGTCTGTTTGGAATAATCGGAGCAAATTTTGATAAATTAGGATTTTTAAATAGTTCGTCTTTATTCTTTATTATTCCATTAAGTCCAAGAAAAATTCCTTTTGCAATTTCATTATTATCATAACCACTGATTTTTTCTTTAATTTTTTCTATTGATAAGTTTTGTTTTTTCAAAGATTCATCAACTTTACTTTTGATTTCAGGATTTTCATTTAATTTATCAATCAATTTGTTAATTGTTTCTTTTATATTATTAACCTTGTTATTTATTTCTTTAACTTTTTTAGGATCATTTAATTCTTCTTTAATATTTTTTGCACTTTGATTATCTTCAGCTTTAAGATAAATATTAATTGCACTTGTCATCTTTTTATATTCATTAGTTAAATAATCATTAAAGTTTCCTGTAGGATTAATTGATTTATAAACTTCAACTATTTTTTCATATTCAGATTCTTTATTAAAATATGTAGCTTTAAGTAATGCAAGACCGTCCTTAGCTTTAAAATCTAAAATTTTTTCAGGTCCATATTCACTTACGTTATTTGCATCAAAATCACTTTGAAGCGCATTTTTAAATGATGCTAATTGGATAATTTTTTCATATGCGTTTTGACAATTTTGAAATTTTTCTTTTTCACTTATTTCATTATTTGGTTTATTGTTTTTAGGCATCGGACTTTCAAATTCAAAGAATTTTGTTGAATTAACATCTTTTTGATTTTTGCCACATGAAACAATAGGAACTAACATGATTGGTGAAGTTAACAATGCTCCAAATTTAAATATTTTTTTTAATTTCATAAAAACCTCCATTTTTAAACACAATTAAATTATATTATTTATTTTACTTTTTTAATTTATCTAATTGTTCTTTTTCTTCTTTAGTTGTTTTTGATAATTCTTTTATTTTTTGTATTGTTTTATTTAAAACATTAATTGAATAATTTCAATTGTTAAGAATTTTTAAAACTTTACTTTTAAAACTCTTGTAAGCTATAGATAAATATCAAGCTTGAACCATTTCTACATAATAATCATTATTGTTAGCTGTATTCATAATTAAATCTAAATAACTTAATTTAGAATCATCTTTTAAATAATATTTCTTAAGCATAACAATAGCAAAACGATTTGTATATGAATTATTATCTCTTAGTCAATCTTTAATATGACTTAAAAAATCTTTTTTATTTTTGTCTTTTAGTTTAAATTCAAAAATATCAACAATAGCTCAATTGTCTAATTTTTTTGCATATTCAACTAAATATTTTATTTGCTTTTTATAATCATTAATTTCTTTAATAAAAAGACCATGAAGTATATTTCCTCAAACAAATAATGATAGGGGTTTCACATAAATTCTGATACTTTTGTAGGTATTTCTTTATATCATTTTTTAGCTAAATCTTTAAGTATTGGAATTCTAACTCCTAATAATTTTGCACTTTCAATTTTATAAATTTGCAAGCAAAATTCTTTATATTTTTGATCAATATAAGGTATTAATTTATAATAAAGTTTTTCTTCTTTTCTACATCTTAATTCATTCATTTTAATACCTTACTTTTTTTCTTTAAAAATTATACTTTAAATAAAATATTTTTTTGTAATATGTTAAATATTAAACCTATAAAAAATATTGCCAATTTTACTAAAAAGACTATAAAACAATTGAATTAAAAATTATTCTTCAATAAAACTTTAAAAAATATATTTATCATTTAGGGACATAAGTTAAAAATTATGTCCTTTAATTTTTGGGACATAAGGATTAAATTATGTCCCTATTTTTTGGTCAAAAACGTTAGTTTACTTTTATTATTTTCGGGACATAAGAAAAAAATTATGTCCCGAATTCTTATAGGACATAATTTCTATTTCTAATTTATTTCTCAACTTTTTGAGCTTGTTTTACTTTTCTTTTTAAGTAAACTCCATTACCAATTGGAATACCTTTTCATCTTCCTAATTCGCTTGGATCAACTTTTTTTCTTAATGCTCCTAAAATAAATGCGCCAGCAAAAGCACCTACAACAAGAGCTAATAAATATCAATATCATCCTTGTGCAACAACGAATACAAAGATTCCGCCATGAGGAGCAGGAATTGAGCATCCTAATCCAGCTGCCATTCCACCAGCTAATGTACTTGCAAAAATTGATGAACCAATAACTCTCAAGGGATCACTAGCTGCATAAGGAATTGTACCTTCAGTAATAAATGCCATGCCCATTAAGTAGTTAACTTTTGAATCTTTTCTTTCTTTAGCAGTAAATTTTTGAGGGAATAAGTCAGCTGCAATAGCGATTACAAGAGGAGGAACCATACCACCAATCATAACAGCTGCCATAATTCATGGTTGATTGATTCCATCAGGATCTACTGAAGCAACACCAATAGTATATGCAACTTTGTTAACTGGACCACCCATATCAACTGCCATCATACCAGCAAGTAAATCTCCCATTCCAATTTTAAAGCCAATTGAAATTGTGTTATTTTTAACAACATAGTTTAAACCTTGTTGAATGTAGTAATTTAAATATGAAAGTGGTGTATTAATTACAAAGAACATAATAACAGCAATAAGTAAGATACCAAATACTGGATAAATTAACATTGGTTTAAGTCCATCTAACGATTTAGGTAATCATTTGAAGCATCATTTTAATCCTAATACTAAGAATCCGGCAATGAAACCACCAATTAAGGCTCCTAAGAAGCCTGCATTAGAACCAGTCTCATAAACCGCAAACTGTGTACTAGATGCCATGGCTCCAGCTACCATACCGACTGCTAAGCCTGGTCTTCCTGCAATTGAATGTGCAATAAAACCAGCAAACACTGACATCATAAAGCCAAAAGCAAATCCACCAGCATTTTTAAAGAATTTTGAAAATGGAGTATATTGTCCAAAATCTTTTCCTACTGCTTCTTTTCCTAAAAAAGAGTCAAATAAGAAGGCTAGAGCAATTAGAATACCGCCACCAATAACAAATGGAAGCATATGGCTTACACCGTTCATTAAATTCTTATATATTTGTTTTCCAAAACTTAATTTTTCTTGATTTGTATTTTCATTTGAAGAAGAATCTGATTTATATACAGCTACTTTTTCATCTATTATTTCTTGTATTAATTCTTTGGGTTGTTTAATTCCTTTTGTTACAGTTGTTTGAAGCAATCTTTTACCATTGAATCTTGTGATTTCAATATTTGTATCGGCAGCAACAATGACACCATAAGCTTTTTGAATATCTTCATTTGTCAAAACATTTTTAGCTCCACCTTAACCTTGAGTTTCAACTTTGATTTGATAACCCATTTCCTTAGCTGCATTTTCTAAAGATTCTTGAGCCATGTATGTATGAGCAATACCAGTTGGACAAGCTGTAACAGCTACTATAAATTTTTCACCATTATTAGTTTGTTCAAATTCATTCTGATCAACTTCTTGTTCTTTTGTTTCAATTAATTTTAGAAATTCTTTGTTGCTTTTAGCATTAATTAATTTTTCAATAAATACTTTATCAACTAACATTTTGCTCAATTTAGCCAAAACTTCTAAGTGTAAGTTTTCACTATCTGTTGTTTCTGGAGCTGCAATTAGAAATAGCAATTTAACAGGTTTATTATCTAAAGATTGATAATCAACTCCGTCTTTTAAAACAAGTGCACTTAAGTGAGGTTTCTTAACGAAAGATCCGCGTCCATGAGGCAATGCTAATTCATCGCCCAATCCAGTTGTTGTTTGTTTTTCTCTTTCCAAAACCGCTGTCAAATAGGCGTTTTTGTCAATTATAATGTTATCACCCATCAAATTAACTGCTAATTCAATGGCTTCTAATTTATCTTTTGCTTTTGCATTAATATCAATATTTTTTAGAGTTAGAAGTTCTTTTATTTTCACTTTTGACCCCTTTCAAGTAATTCTAATATTGCACTTTTATTAGCTATTTCTTTATCAAAAGCAGTTGCTGCTCCACAAGCTATTGAGAATTCTAAAGCTTGTTTATAATCTTTATTTTTTAAATAATTATCAATAAATCCAGCAATCATGCTATCTCCTGAACCAACTGTACTTACAACTTTTCCTTTATATGTAGCACTATAAAAGGTTTGATTATCTTCACTAAATAAATAAGCTCCTTCAGCGCCACAAGACACTAACACATTTCTGGCACCTAATTTTTTAAGTTTTTCAACATGACTTTTTATGTCTTCTTTAGTTTTAACTTTTTCATCAAATAATTCTTCAAGTTCTTCTAAATTAGGTTTAATCAAAAATGGTTTGTATTTTAATGTATTAAGTAGAAATTGTTTAGTTGAATCTACTATTATATAAATTCTTTTGTTTTTGAGTTTTTCTAAAATTAATTCATAAACATTTGTGTCAACATTTTTTGGAACATTGCCAGCCAAAATTAAAGTATCATTATCTTTAATATTAAAAGCAATCTTATCTAATAATTGTTTTAAATTTTGATCACTGATTTTAGGTCCATTAGCATTAATAGCTGTTTCTTTAGCTTTGCCATTTAGTTTTACATTAATTCTTGAATTACCTTCAATATTACAAAAATCTGTTTTAACTTTTTTATCTTTTAAGTAATTAATAATATATTGACCAGTAAAACCACCGACAAAACCTAAAGCTACATTTTTAGTTCCTAAATTATTTAATAGTATTGAAACATTAATTCCTTTACCACCAACTGTATAATTTTCACTTTTGGAGCGATTAATATCTCCTATATTTATTTTTCCCTCTAAAGACATTGTATAGTCAATAGCTGGTGAAAAAGTCACTGTGTAAATCATCTATCCTCCTCAATTTAATTCTATTTAAAAAACTACAAAAAAGCACAGAAAAACACAAAAAGTTACAAAAAACAACAAAAAATAGGCATAAAAGCCTATAAATTTGTAGAATTTTGTAGAATTTTCCACATTTTCTCACATTTTATACTTCTATGATATTCTCAAGATTTAGATCTTTTTTCTTGTAATTAGTTATCAATGTGATGTTTTTATTATTGGTTGCAAAAGTATATTTAAATTCAGAATTTATTTTGGTTTTGTCTAACAAAATATAACTTTTTTTGCATTTTAAAATTGCTTCTTTTTTAAATAAACTTTCATTAATTTCAGGTGTTGTAAAACCTAGATTATTGCTATATCCATTTGCTCCAATAAATCCAATATCAAAATTAAAGTTTTTCAAATTATCTAAAGCTAATGATCCAACAACATTAAACGTATTACTTTTTAATAATCCACCTATTAAAATAGTTGTAACGTTTGAATGACTTTTTAATTCTGTAGCAACATTAATTGAATTCGTAACTATAGTTACATTCTTGTTATTAATTAATTTAGCTAATTGATGAGTTGTTGTTCCTGAGTCAATAAAAATTAATAAGTTATCTTGTACAAATTCATTTGCTTTGGTAGCTAATTTGATTTTTGCATCTAATTCAATATTACTTCTTGATTCATATTCAACATCATTTGCTGAAGAATTATTATTTAATGAATAAGCTCCGCCATGAACTCTTTTTAATAATTTTTGTTTATCTAATTCAGCAACATCTTCGCGTATTGAAGAAATGCTTGCTTGAGTTAATTTTGCAAGCTCTTTTAGTGAAGCAGCACCTTTTGAATTTACTTCTTTTAAAATAACATCTAGTCTTTGTTCTTTAAGCATAATTTTCCTTTATTTATATTTTAATAAATATATTTAAAAAATAAAGTTTTTAACTTTTAAACTAATTATTAAACATTTCTTTTAATTTTAGGGACATAAGGATTAAATTATGTCCCATTTTAAAATCTACAATTTGGAACATAAGAAAAAAACATGTCCCTAAATTGTTTAAGGACATATCTATATTTTTATTCATTTTAGGACATAAAAAAATTTATGTCCTTCTTTATTATTTGTCTTCTTTTTTAATTTCTAAGAATAATTCATCTAATTGTTTTTTATCAACACTGCCTGGAGCTCCTGTAAAAACATCTTGGTTTTTAGCATTCTTAGGAAATGCAATAACATCACGAATTGTTTTTTGACCTGTAATAATCATTGTCAATCTGTCAAGTCCTAAACCAATTCCACAGTGAGGAGGAATACCATAATCAAAAGCTTTTAAGAATCAATCAAATCTTGATTGTTGTTCTTTTTCACTCATACCAATTAAATCAAACATTTTCTTTTGTGTTTTTTTATCAAATATTCTTGCTGAACCTGAACCTAATTCAAAACCATTTAAAACTAAGTCATATGAAAGCGCTCTAACATCCTTCATTTCAATTTTGTCTAATTCTTCTAGTGAATGCGCAAATCTAGTAAAAGGGTGATGGGCTGCTGCTCAAGTATTATTTTCTTCATCATATTCAAACATAGGTCAGTCTGTAATTCAAGAAAGATTTCATTCCTTTCTTGCATAATTAAATCTTTCATTTAATTCAACTCTAACTGCACCTAAAGCTTGAGATGCGTGATCATAAGTATTAGCAACTATAAAGTAAGAACCATTTTTGTTTTTGTTATTTTTGACTAATTCTAAAGCTGCATCTTTAACTTTGTTTGCAAAGTTGGTGTGAACTATTTCACCATTTTCATAAACAAAGTAGAATAGAATATTTGCTTTATTTTTCTTAGCAATTTCTTCTAAGTCTTTAAAGTCTTTTTTGGTAATTAAAGTATCTACAAAAAGCATTCTTTTACTTGGAGCATATTTAATGATATTAAAGTCTGTATTAAGGCAAAAGTCATTGATGTCAACTATTTTATAATCATATCTTAAGTCAGGCTTATCTGTTCCATAATCTCTTAAAGCATCAAAATATTTAACTCTTGGAAATGGAGTCTTGATTTTAATTCCTATTGATTTAAAAATGTGTTTGAATAATTTTTCAATGTATTTTTGAAAGTCTTCAACATTTAAGAATGAAGTTTCAATATCTAATTGAGTAAATTCTGGTTGACGATCTTTTCTACTATCTTCATCTCTGAAACATCTTGCAAATTGATAATATTTTTCAAAACCTGAACACATTAAAAGTTGTTTAAATAATTGGGGGCTTTGAGGTAAAGCTCAAAATTCATCTCTATTTCTAGTAGGTACTAAAAAATCTCTAGCACCTTCAGGTGTACTTTTAGCAAGCATTGGAGTTTCAATATCAATAAAATCATTTTGGTGCATGAATTCTCTAACAGCAAACATAATTTTATCTTTAGTGATAATGTTTTGTTGCATTACTGGACGACGCAAATCTAAGAAACGATATTTCATTCTGGTTTCTTCTTTAACATCAATATCATCTCTAATTGCAAAAGGTAATTCTTCATTTGCTTCAGAAAAAATTGTGTATTTATCAACTACTACTTCAATATCACCAGTAGCTAGATTAGGGTTCTTTTGTTTTCTTAAAACAACCTTCCCTATTACTTCCATTGAACTTTCTTTTGTGACGTTTAAGTCTGTTTTAAAGACACATTGGGTAATTCCATAGCGGTCTCTTAAGTCAATAAAAGTCATTTCTCCAAAACGCCTTTTATTAGCTACTCAACCATGTAAAGTTACTTTTTTACCTTTGTCTTTAGCACTTAATTCATTGTTCTTTATATATTTACTTTTCATTTTTTATTCCTCGTCTTCATCTTCTTCAGCTATTAAATCGCTTAGTAAATTATGTTCTGCTAAAAAGTCTAATAAGTCTGCATAACCATCTTCATTGTATTGGAACATAATACGATCATTATCGCTTAAACTTTTAGCAACAAAGGTTTCACGACCTTGAATGTTGTCATCAAAAATCACAACATCAGCTTGTAATTTTTGAGCCTTATCAAAGATTTTTTTGCTTTTAACTACTTCTTTAATAAATTCAATTTTGCTTCCATAATTTCTTAGCTCATTAGTTAATGAAAATAAAATGTCTAGATTTTTTTCACTGGTTGTGGCTACTAAAATGTTTATTTTTTCAGCATCATTATTGTCTTTGTTTTCATCATTAATAATGTCAGCTGCTCGATCTACTCCAAAACCAAAACCGCAAGCATATAATTGAGGTCCACCTAATTCTTTGATTAAGTTCGAATATCTTCCACCGCCAATTATTGTACTTTGACTTCCTGATTCTTTTGAAGTTGAAACAAATTCATAAACAACTTCATCATAATAATCAAGTCCTCTAACTAGAGAATAATCAACTTGATATTTGATGTTGTTGAAGTCTAAAATTGAAGTTAATTTATTAAAAAATTCAGTGCTGTTTTTGCTTAAGTATTTATTAATTTTAGGAGCTTTTTTAACAAAATCTTTTTGCCTTTCTTCTTTATCATCAAGAATTCTTAAAACATTATTTTCAAGTCTTTCAAGACTAATTGGATTTAATTGATCTTTATATTTAGCAAAATATTTTTTTAATTCTTCTTGATATCTAGTTCTGGTTTCTTCATCACCAATTGAATTAATTTTTAAAACACATTTAACATCAAGACATTGTAATAGTGAAGCTGCTGCAATAATTAATTCAGCATCATTATATGGATTAATAACTGTATCAACTATTTCCATCCCTGCTTGATTAAATTGTCTATAACGTCCTTTTTGTGGTTGTTCGTAACGAAACATTGGACCATAATAAGCAAACTTTGTATTTTTAAGTGTTGCATATCATTTGTTTTCAACTAAAGCTCTAACAAAACCTGCTGTTCCTTCTGGTCTTAGTGCAATATCACGATCACCTTTGTCTTTAAATTCGTACATTTCTTTTTTAACTATATCGCTACTTGCAACTGAACGACGATAAAGAGTGGCTGATTCAATAATTGGAGTTTCAATTAAATGGAAATTATAATTTCTTGCATAATTAACAAATGCACTTCTAATTAATTCAATCACATTATTTTCTTGTGGACCAAAGTCTCTTGTTCCTTTAATTCGGTTAAACATTTTATGCCTCCTAAAATAAATACTCAATTTAATATTAAATATTATATATAAAAGTTAAATAAATGTTATGTTTTTAGATTTAATAAAGTTAATATTTGCTTTTTTACTAGCTTTTAGTATCTTATTTTTACAATTTATGTACTTTTGATAAAATATAAAATAACTATGAAAAAGGCAAAAGATAATTTAAATCAAAAAGTTAATAAAAGAAAGAAAATCAGCTTCTTTACTGCTATGCTAATAGTAATTAGTGGCAGCAATGGTGCTGGTATTTATTTTCGTAGCGAAAGCATACTTAACAATTCACATCAGAGTTTAATTTTAGCTATCTTTTGTTGAATTTTTGGTGCTTTAGCAATTATTGCTATGGCTTTAGCTTTAGTTGAAATTACTAGTGCTAAAAGTCATAATTTATCTTTAATAGGATGAAATCATACTTTTAATAATCGGCATGTATTTAATATGTCAAAAAACTTTATGACTTACTTATATTTGCCACTTAGTTATTTTTTAATGCCTCTTTATGCTTTAATGTCCTTACAACAAGGAGTAGGAGCAATATTAAATCAAGAAGCGTTTGTGATTGGCACTAAATATGATTGAATGATTTGAACAGGAATTAGTTTATTGATTGTAATTTATTTTTCAATAGTACCAGCTCTTTATACTAAAGCAGGTGATATTCAAAATAAAATTATGTTAGCTGTTAAATTTTTGCCTTTATTCTTTATTATCATTATTGGTTTTGTTTTGGCTTTTAGTTCAAAAGGTGAAGTAAATCAAACTCATTGGAATTTACAAGATCAACACTTTGATGTTAGATTGGGAAGTTCAATTGCTTCTTATAAATCTTTAGGTGCGGGCTTTGGAGTATTTTTATCAGTATCAGCTATCTTCTTTGTTTATGATGGTTTTTATATTTCAGCTGGTATCCAAAGCGAAATGAAAGAACCTCAAAAGACTCCTTTAGCATTATTATTTGGTTTATTGATTACCACAATAATTTATCTTTTAATTGCCATTTCAATGAGTATTAATGGTGGTTCATTTATTAAAATGAAGGATTATTTAAGTAACTGATGAGGTTCAAAAGCTGGCAGAATTATCTTTGGTATTTTAAACATTTGTATAGCTATAGGAATCTTAGGAATCATTAACAATTTAGCAATGTGATTGCCTCGCTATATTGAAGATTTATTGGCTTTAGGTGAATTACCTTTTTGATATAAACTTAGATTTAAATTAAATAAAGAGAGACCTAAAATTGGTGTAATTTATAGTTTATTTCTTTGTATTCCTTTAATTATTTTGTTTTCAGTTTTTGGAGCATTATGTTATTTTTCAAATGAAAATTACAACATCTTTAATAGCTTAAATAGTGATAAAACTATTAAATCAGATGCATTAAATAAGTTATATAACTTTACTGATTTAGTGGCTAACTGAACATCTTTAATTGTTTATACTTCAATTGCTACAGCAATACTTGGTGGCATTAAAAATAGAAAACATAATTTTGTAAAAGCACAAAAGAAAAAATACTTTTTAGTATCGGCTTACTTTATTGTTACAGTTACTTATTTATCTGTAATAATCACAACAATCATTCCTTTTATTGATTTATTTTTATTGATAGGTTTTGACAGGGAATACTTTAAAACAGTCGAATTGGAAAAATTTGCAAAAGATAATACTTACATAATTAGAAATGAAAATACATTCTTTAATCAACTGCTTAAAGGCAGGATTTTATGTGTAGTAACATTAATAATTTATTTACTTATTTCAATATTTCCAAGTGTTTTTGAAAAGATATATTTCAAGAAAATGTTCAAATCATGAGATCTTTATTTAGCTTGGAAACAAGAAACACTTGAAAAAGGAATAGTTGTTTAAAAATAAAAAATATCACTTAATAGTGATACTAATAATTAGCTCTCATAATGAGAGTTTTATTTTATTTAAAATCTAATAATTGATCTCTGTAATACTTAAATTGTTTACTTCTTAATTCTATTTCGGCAGGTAAACCTAAATTAATTTCTTGAGAGTATTCTGATAATTTGTCAAGAATTGAAACTATCTTGTTTTGGGTTTCTAAAGGAGGAATAGGTATATTTAATTCCAAAATATTTTTCATAAACGGATGCTTTATACTACCACCTCTAAAATTTTTTCTAATTAAATCAAGATTTAAAAGAAAGTAATAATATAAAAATTTTGTTGCAACTTCATTTTTATTTAGAACTTGTCCAATTCTATTATCTCCCGTAACAAATTTACCTTTATGATATTTTATATTTGCCTCTCCACCTCCGGGAATTAACAACACTTCACCTGAATATATATCTTTTTTATAATTTTTACTATTAATTTTTAAATTTTCTTCTTTGCCTGTGGTAATAATTTTTACATCGCCTTTAGGATCATTATAATTTTCTATTTCTTTATAAAATAAATATTTAAAATTAGATGTTTTACTTTGTTTAAATTTTTCAACTTCTTGGAAGTTTTTATCTCAGAATGTTATTTCTCATAATTTTTTTAATTTGTACTGTTTGAAATTAAAAATTTTTGTTAAAGTGAAATCAAATTGCTTCCCTCTCGCTTCCAACTCCGCTTTCAGCTCCGCTTCTAGTATACGAAATCTCTCCAAGATTTCGACAATTTTTTCTTGAATTTTTAGAGGAATTAAAGGAATTTTTAGTTTTTTCAAAATTTCTGAATTAAGGTTTGATATTGTCCCCGGTGAGGATTGTTGAAGAAAAAATGATTGTGAATAATTAGATAAAAATCAATAATATAAAAATTTTGAAGTTACATTTTTTTCAAAATTTGCTATTAATAACCAGCCATCATGAATACAACCTTTTATATCAGCAATCACTGGTTTTCCAAAAGACATTGAATTAGATAAAATTAAATCTCCTTTATCAACAACAACAGATTTTTTGCTACCTTTTTCGTTGATAAATTGTTTAGTCTTTTTTAAATAAATTTTTCCTTCTTCTATATCTCCAATTTTTATTCATGGATAACCTTCTTTAGACAAAAAAGAAGATATAGGTCTAGGAGAGGCACCTCTCCTAATGCTTGATATTTCGCCCAATGTAACCCACTCATATCCATCTGGACAATACTTATCTAATAACTTTTGTAAATAACTCATTTTCTTCCTTTATTAAAAATATATTTTAAACTATTATTTTATTAATGCCTTCCAATAACCATTTTTATTTGCTCCAACTCTTTTAATAAAACCATGTTTTTTTAAAAAATTAATATTGTTGTCTATTGCTGTTGTGCTTAGATTTAATTTTTCTGCTAATAAAGCTTTAGTAATATGTGGATTTTTAATAATTTCCGATAATATCTTTTTTCTTATTTGATTAAGAATTTTTTTAGCTGTTTCTTCATCTATCTCTTCAACTTCAATTTCAAATTTTACAGCATTAATTCTATTAAAAGGAATTGTAACTTTAATAAATCCATCACCAAAATAAATAGCTTCTTTGCCATAAACTTTTACTATTGCAGGTACTCCACGTCCTGATTTTTCACTAATTCTTAATTGAAGAAAAATTTCGGCTAACTTTTCATTAACTGGTTTTGATTCTCCTTTGTAAAAACCTTCTATTGTTTGTTCAGGTCCTAAATTTCCCATAGAATCAATTTCAATTCTATCTTTAAATATAGAAATCATTGGAGAAGCTTTTGAAGTTCATTTGTTATGTAAGATGGCATTAATTATTGCTTCCTTGTAAACATTAGAATCAAATAAAGGTACTTCTTTTCTTGTGAGAACTCTGTTTCTTTCATCAGCTTGATAAATATTTACTACATTTCCAAATCTAATCAAATCATCTAGTGTATAAAGTAAATTAGTAAATCCAAATTCATCTACTGTATATAAAGGGGTTGTTTTGTCATAACCTCCAAAAATAGAAACTCTAACTGGAAATCCAGAATTATCTGACAAAAGCTGAGCAAGTAAGTTATACTCACCTTTTTCGGTATAAAATTCAAGGTTTTGTCTAAATGTTTCTTCTTTTAATTGAATTCCTCTTGACCCATAAAATCCAAATAATTTATTAAATGTTAAATCTTGAGTATTGGCTTTAGTGTTTTGAATTGTTTCATGGCCAAAAATTAAAACTCAAAATAATTTTCTTTCTCTTTCAGGAAATTTCATTAATTTTTCTTTGCTTGATCCAACTCGAATATATCTTTCATTTTTTCAAGCTGTTGGAGTTTTATGAGCTGCAACTATTTTTAAAATAACCAATCTTTTGCCTTGATAATTGAATTCGCTAAATTCAATATTAATTCTTGGACTGAGTTGTCTTTCTAAATAATGAATTAAAGGCTCATTACTCTTTGTATGACTATCATAATTAAATTGTGTACCAACAATTTTATGAGTTTTATCTTCAATACCTCAAACTAAATATCCATAGTCTCTGCCTTCTATAGCGCATGAATTTGAAATAGCAGAAATATAAGAGCCTATTTCATCTTCATCAGCTCTATTAACTTTAAATTCAAATCACTCCTTTTCAAATTCATAAGAAGTTAATTCTTTAATTAATTCATTATTTGTCATGTTTTTTTTCCTTTTATTTTATCGATTTTATTACCAACTTTATTACCCACCTTTAGTATATCAAAATTTTAATTAAAAGTCAATTAAATAGTAAAAAAATGATTAATAAAAATAAACCGTATTTGCCTATAAAACAGGCTTTTTGGTTTATTTGAATTGATTTTCATATAAATTAAAGTAATAACCTTTTGCTTCCATTAATGATGCATGATTTCCTTGTTCAATAATTTGACCATCATCAACCACTAAAATTAAGTCCGCATTTTTAATAGTACTTAAACGGTGAGCTATAACTATTGAAGTACGATCTTTCATTAATTCTTCTTGTAAAGCTTTTTGAATAATTTGTTCAGTATTTGAATCAACATTACTTGTGGCTTCATCTAAGATCAATACTTTCTTTTTACCCAACAGTGCCCGAGTAATTGATAACAATTGACGTTCACCTTGACTTAAACTTTGACCACTATTTTCTATTACAGTGTCATAACCTTTTTCAAGTTTTTGAATAAAGTTATGAGCTGAAGTTAAACTAGCCATTTCATATACTTCTTGGTTTGTGATTTTGTCTGAAGCTACTTTTAAGTTGTCCATAATGGTTTCATTAAACATAAATGAATCTTGTAATACAACTGACATTAAGTCTCGTAAGTTTTTCTTAGTAATTTTTGTTAATTCAACACCATCAATTTTGATTGATCCTTCTTCATATTCATAGAATTTACTAAGTAAATTGATTATAGTTGTTTTACCAGCACCGGTAGGACCAACTATGGCAATAATTTGACCTGGTAATGCTTCAAAAGTTGCATTTTTAAGTTGATATTTACTACTATTTTTATCATATCTGAAGCAAACATTTCTAAATTCAATGTGGCCTTCAATATCTTTTAATTCAATAGAATTTTTGATTTTGGTTGGAAGTTCTAGTTCTAATAAACGGAAAACACGTTTTGTTGAAGCAAGTCCAATTTGAATTGAAAAAATAACATTGAAGAAAACTTGTAAGGTACCTGTATAGTTTCAAAGCAGTGATGTATAAGCTATCATGAAACCTGCATCAGCTTTACTAAAAAAACTAAAAATACTTAAGGTAGGGACATTTTTAATATTAAAGATAATGGCAAAGAGTGAGATTAAAAGAACTAAAAGGTTGGTTGAAATAATAAATCAAGGATCAAAAAATTTAATACAAACATCGCCTTTGAATGAGTGTCAATAAATATCATGAGTTATTTTTTTGAAATTCTTTTCTGCTGTTTCTTGACGATCAAAAGTTTGAGTGATTTTGCTATTAGTTAGCATTTCTTCAACATAAGCATTTAAATCACCAAAGTCTTGTTGTACAAGAACATATGATTTTCTAGCTTTTGAAATCATGAAGTAGCCAAGTGAGAAGAATAAAACTGATAAAGGAATTACTATTGCTGTTAAAGTAAAACCGACAAAAGCCATTGCTAATATTGAAAGAATGACATTGAAAATGTTATTTCAAGTTTCATTGAACATTTGAACAAATGAGTTTGCTAAGTTATTTATATCATTGATTAATACTGAAATTAAGTCACCACTTTTTTCTTTATCATAAAAGCTAACTGGCATTTCAATAAGTTTTTCCATTGCTACTTTTCTCATTTTTGTAGCAGCTTTATAAGCTATGGCAATTAATATCCGACTTTGAATAAACCGTACTATTGCATAACAAATAAAAAATATTGCTAAAATTGAGTTTCAAATAATAAAGCCTTTAACATCGAATGCTCCTGGAATAACTTTACCACTAGCATCAAACATTTTTTGGCTGAAAAAGAGACTAATAATAACACCAGTTAAAGTAGTTCCTCCAACATAGAAAACAGCATTTCAAAAGCAAATAAAGCCACCAATAATGACTAATTTTTTATCTTTTTTGTCTAAGAAATCATAAACTAATTTAAACAGTCTTCAAGTTGAAAATTTTTTTTCTTTTTGTTTATTTTTAGCCATATTGATTATCTCCTTTCTATTGTTCTAGCTGTGTTTCATAGATCTTTTGGTAAAAATCGCAGGTCTTAATTAAGTGACCGTGTTTACCTTGATCCATTACTGCTCCATTAGTTAAAACCATAATATTGTCAGCATTTTTAATAGCTCCAATTTTTTGGCTAATTAATATTGTTGAACAGTCATAATTATTTTTAATATTATTAATAACTTTTCTAGTTGTAATATTGTCTAGTGCACTTGTTGAATCATCTAAAATTAAGATTTTAGGTTTTCTTAATAAACTACGAGCTATTGAAATTCTTTGTTTTTGACCACCGCTAAGGTTTCTAGCTCCTTGAACAACTTTGTGATCTAAACCATCTTCAAATTTATTAACAAAATCATCAGCACAAGCATTTTTAAGTGCTTCACGCATTTCTTCATCAGTTGCATCAGGTTTAGCTCAAAGTAAGTTTGATCTAATTGTTCCAGTGTAAAGTAAAGCTTCTTGGTAAACTATGCCAACAGTTTCATGAAGGTTTTTGGTATTAATTTCTTTAACTTCTTGGCCTCCAATTTTAATTGAACCTTCATCATAAACATAATTATTTAAAAGTAAGTTAATAAATGTACTTTTACCACTACCTGTTGGTCCAATAATACCTAAAGTTTGTTTATAAGGTAATTTAACATTTATATTCATTAGTGAATATGAAGGGCTGGTTTCATAGTAACGAAATGAAAGATTTTGAACTTCTAGATCAAAATTATTTGTTAATTGTTTACCATCTTTAATAAATAAAGGATCTATTTTGGTTTCTAAAATTTCATTAATTCTACCTGCTGAAACACGAGCTCTAAACATTGATGTTAAGAATTGAATGGTCATTAAGAGACCAAAGCTAATTGAAAATAAGTATTCTATAAAAATATTAACTTTAACAAGTGAATCTAAAGTTGCAACTTCAGTATACATTCTATGACCTGCAAAAGCATAGATTCCAATAATTAATAAGTTAATTGAAGCATAAAAAATCGGTACAGCAAAAGCAAAAATCATATTTGTTTTGAATTGCACTTTATATCATCTGTGGTTGTATTCACTAAAACGTTTTTTTCTTGTTTCTTCTAAGTTAAAAGTTTTAATAACTCTTGCTCCTAAAATATTTTCATCAGTTGCTTTAGTAATTTCTTCAACAACTTTTTGTGTTTTCTTTAAAAGTGGATTAGTAGTTAAACCAATAACAAGTACTAGTACAAAAATTACAGGTACAACAGCTAAAACACTAAGTGACATTTTTGGATCGTTTAAAATTGCTAAAACTCCACCACCAATTACTAAAAAGAAACCTCTAATTAATATACTTGAACCATTAACAAGGAATTCTCAAAAGATTGCAACGTCATTTGAAACTCTAGTAATAATACTTTCTGGTTTTAAATCAGCAATATTTTTAAGTGATAATTTTTGAATTTTATTAAACAAAGCATTTCTAAAAAAGCGGCTACTTTGTTCAGCTGGTCACACAATCATAAGTGTTGAAGTAAAAGTTAAAGTGGCTGCTAAAATTGTTTGACAAACAACAGCAATACTTAAATATGTAATTAAATCTTTACATGTTCCTCTAACTTCTCAACGACCATTAAAGAAGGATAATGTCTCAACTTTTGAATAATCATCAACGAATAATAATCTAATAAACTGAGAAATAATATTAGGCAAAAGCAAAGTAAAAATAATATTAATAAAAACAAGCAATGAACCAAAAAGAAATGCTAATTTAATCTTAATTGGTAGCATTTTAATTAACTTAAACATTATTCCTCCTTGCAATATATAATAAATACTTTTTCTATTATAAAACTTTTTAATTATTAATATATATAAAATATATTTAAAATATTAGAATTTATATTAAACTATAATTATTAAATATTTCAAAAGAAATGAGGACTAAATATGAGTGAAAAATCATGTGGATTAGTAATTTTTAGACAATTTACAAAAGGTTGAAAAGTTTTGATTGCTAAGCAAATAAATAATATGTGAAGCTTTGTTAAAGGACATGTTGAAAACAAAGAAAGCGAAATTGAAACTGCACTAAGAGAAGCTAAAGAAGAAGTTAACTTAGAAAATTTTCAAATTTTCAAGGAAACTAGAATGAAAGAAAAATATGTTTTACCTAATGGAATACCTAAAGAAGTTGTTTACTTTTTAGCAATGCAAACTGATAAAAAAGATCCTCAAAGACAAGAAAGCGAAATCTTAGAAATGAAATATTATTCATGTTCAAGAGCTATGAATAAATTAGTTCACAACAGTCAAAAAAGAGTTTTAAAAACAATGTATAAAAAGTTAAATCAATATTTGCTTGATGAAAAAAAGAATAAATAAAGAATCCTTTATTAAATACTTGAAAGGATTCATTTTTTAACATTAATTATTATTAAAACAAAATAGGTTTGATTAAGTCTCAAATTAATTTAGAAGTGTCTTGAACTAATAAAGTTGCTTTTTGGAATAAATTATTATTACCGTTTGTTATTGCAATGGATTTAATGTTAGCCTTATTTAAAGCTTCAACTCCAACAACTGCATCTTCAATTCCAACACATTCTTCTTTTTTTAATTTAAGTTGTTCTTGAGCTAAAAGAAAAATATCTGGTGCAGGTTTTCCTTTTTTAACTTCAACAGGATTAACAATAACATCAAAATAATTTATAACTCCGAGTTTTTCTAAAATCAAAGGAGCATTGAGACTTGAAGAAGCTATTGCAATTTTAATCTTATCTTTTTTTAAGTCTTTTAAAAATTGTTCGATGCCTTCTAAAAGGCTATCTTTATTAATATATTTTTTTAAATAACTTAAATATAATTCATTCTTTTTAGTACATAATTCATTGATTTTTTGATCATCAAAATTATGCTTAAATTTCTTTAATTCAAATATTGCAAGTAAAGTATCTCTACGAGGCAAGCCTCTTAATTTTTCATTTTCTTCTTTGCTATAATCAATACCTAATTCATTGACTATTTCTTTTCAAGCTTTGTAATGTAATTTAGCTGTATCGGTAATTACTCCGTCTAAATCAAAAATTATTCCTTTAATCATTTTTCACCTCGAAAACTTGCTTTTCATTTGTAATTTCACAGTCCATACCATTAACACATATTTTTAACTTAGAACCTTTATTTATAGTATTAATGGTAAATTTGTCCTTGTTGATTTCTACTAAAAATGGCTCATTTTTGTAAATTGCTTTATAAACTAAACTATTTCAATTATTTGGCAATTTTGGAGCTATATGGAGCTTTTTATTAAATCATCCTAAACCACCAAAACCAAAGACAATCATTTGTCAGATTGCTGCTAAAGATCCTGCATGAATACCTGCATTTGAAGTGTGCATTCATGGACCAAAGTCAATATTAATACCATATTTAAATAATTCATAAGCTTTTTCAATTTTATTTAATCTAATAGCTTCAATACAATAAGTTGCTGCCGAAAGTGATGAATCATGTGTGGTTATTGCTTCATAGTAATCAAAGTTTTTTTCTCTAATTTCTTTATTATATAACTCAGGGAATAAATAAGTTAAGAGAATGACATCGGCTTGTTTTACTAATTGAGAGCCTAATAATCTATGTCCTTCAGAAGTATTGAATAATTTTTTACCAGCATCTCCAAGCATTTGAAATGGTCTAACATTTTTTAAAGGTAAGGATAAGAATGTATCATTTTCTGCAATAATTTTATCTTTATTAGGAGCTTGAATTTTAAGACCTTTAATGACTTTAGTCATTTTTGAAAAATTAATTTTATAAGGTATTCTTTTTAAAATACAGTTAAGTAAGCCTGGTGTTTGTGCTTGCAATTTGCGATATATTGAAATTGCCAATTTCATATTAAATAAGGCCATTTGGTTGATAAATGCATTATTATTAATGTTTCCTTTATATTCATTTGGACCCATTACATCTTTAATTTCATAACTACCATCTGGTTGTATTTCTGCACGGTTTGTGTAAAAGTAAGCTGTATCAATTATCATTTCATAACCTATTTTTTTCATGAATTCTTCATCATTTGTAAAATGATAATATTGATAAACTGCATAGGCTATATCAGCTGAAACATGGATCTCTTGTCTTCTACTTGCAATCGGTACTTGTTCACCTGTTACAACATCAGTTTGGCCTCAATAAGGACAAACTTCACCATCAGTAGGTCAAGCCATTTCTCAAGGATATTGAGCACCTAATAAATGACTTTCTTCATTTCTTCGTTTTGTTTCGAAAGCTTTATTTCTAGCTCCTTTTATCCCTTTATAACGATATGTTAGTAATCTTTTAACAACACTTGGTCTAGTAAAAAGATAATTAGGATTAATAAAGAATTCAGTATCTCAATATGTGTGCCCTTGGTAACCTTCACCTGAAAGTCCTTTGGCTCCTACATTTAAGTTAGTTGAATTTTTAGGCACAAAATTATTTAAGTGAAAAATTGAAAAGTTTAATGCTAAAGCATCATATTTACTTTGTTCATTACCTTCGATTTTGACATAAAATTGATTTCAAACTTTTTGCATTGCAGCTGTAGAATTTTCTTTTAATGAAGCATATGTATTTTTTAATAAGTAGTTAAAAGTTAAATCTGATTTAGCCAAAACTTCACTATTAGTTAATATTTTTTCTTCATCATCAATTGATGTATGAACTGACATTAATTTTTCTAAAACTAATTCTTCGTTTGCTTTTAATTCATTTTCGATTTTAAAGTAAACTTTGCGGCGATCAGTTTGAATTTGATATTTGTCATTACTGCCTAAAATTAACTTATTATTTTTTAAAAGTCTTGTAAACATGTGGTGCACAACAAAACGATTTGAATTAGTTGTTATTTGTTGCATCTTCAAACTTTCGGTTGTCACACGGTTTTTAAGACCTTCTTCAAAGTGTTGAGTACCACTGTTTGTTACTTGTCCATTAATTCCTGGTAATAATTTAACCTTAACAATTTCATCTTTAGAATCTAAAATTTTAATTGTAATTTTTTGAGCATAAATGTTTCTTATTTCTTGAGAAACAAAACGTTCAAAATTTAATTCAATTGTAGCAAAGTCTCTTTTGGCAATGACTTTACGACTTAAAATTCCATTTTTAATATCTAAAGTTTTAGTATATTGATCCTTTTCATTAACTTCAAAAATTTGATTATTAAAAGAAATAGGAGTTTGAATTAAATCAGCTAAATTAGCTAATTCAGGAACTTCTCCTTTAGATGTTCTATTAAAAATACCATTAACAAAAAAGTCTTCTTTGTTATATTCTTGAACTTCTTCATCAACACTTCTAATACCTAAATAACCATTACCTAGTGAAAAAATACTTTCACTTTTTGCTGTTATATTACGGTCAAATTTAATCTGTGAAATCTGATTATTATCAGGGTCATATTCAATATATTCCATAATTACTCCTTATTTAATTTCTAACATAATCGACTCAAAAGGTCTTAATTTATCAGTAACTTTTTTGTTATCAATATATGTTGATAATATTTGTTTTCCTTGAACATTTTCAACTACTTTTTGATGTTTTGTCATATTAATTAAAATAACTATTTTTTGCTTATTAAATGTTCTTGTAATCTGTATTATTCCACTGTTTTGTAGGTCTATTTGACTTTTTCCTAATACTAAAATGTCATGATATTTTTTAGATCTTAGGGCTATAATTTTTTTGTAAAAATTAAGAATACTATTTTTGTCTTCTAAATTATTTTGAACACTAATTCATTGTGCTCTTTGATTTGTTTTAATTCAAGTTTTTTGGCCTTCATTAAAACCATTGTTTACTGCATAATTATCTCATTGCATCAAAGTGCGGCCTGAGTCACGACTGTTGATATTGCAATATTTAATAAATTCGTATTCACTGTAAATTTTCTTTTTATCTACTAACTCACTAAAGGCATTAAATATGTCACAATCTTTAAATTCTTCTCTTGAGTTAAATTGATTATTTAATAAACCAATTTCTTCTCCATAATAAATACAAGGAATACCTTTTAGCCCAAAAAGCATTAAAGCATGACTTTTAGCACTTTCTTTATGAAATATGTTTTCATCTCCTCATCTAGAAATACTTCTTGAAGTATCATGATTTGATAAAAAATTGGTTGTCATACTTGGTAAAACTTTTGTTGAATGTTGAAATGGTTGTTGTTGATAAACAAACTCTTTGTAATCTCAATTTGAGTCATAACCATTTCTACCTGTTTCTTTTCCTCAACCAATTCATCATCAAGAAAAGTTGTAATAGTTATCAGCTACTTTTTTAGATCCTGTTCCATATTTTAATAATTCTTCTAAATTAATTCCACTAGCTTCACCTAATGTATAAGCATCTTTTTTATCACTAAAAGCTAACTTATTAAACTTAAGAAGCATGTTGTCTGCACCTTTACATCATGCAAAACAAGAATTAGTTAAAGTTTCTTCAAAGTTTTTTGAAACGTGTTTAATAGCGTCAAGTCTAAAACCTCTAACACCTAAGCCATATCAAAAATCAATAACATCTGACATAGCTTTAACTGTATCTTGATGATTTCAGTTTAAATCCACTTGTTCTTTAGCAAAAAGGTGAAAATAGTATTTTTTAACACTTGGAACATATTCTCAAGCTGAACCACCAAAAATACTCTTTGCTTCTTTTTCTTCTTTATTTAATTCTTTTCTTCAAATGAAATAATTGTGTTCAATATTATCTTCTGATTCACAAGCTTTTTTAAATCATTCGTGTTCATTTGAAACATGATTTAAAACAATATCCATGATAATATCAATATTTCTTTTTCTAGCTTCAGTAGTCAAAAGTTTGAAATCTTCTAATGTTCCAAATTGTTTTCAAACACTTTTGTAATCTAAAACATCATAACCAGCATCCACAAAATTAGTGTCATAAATTGGACAAAGTCATATCGCATTTATACCTAAGTCTTGTAAATAATCTAGTTTTTTTGCTATACCTAATAAGTCTCCATCGCCATCATTATTTGAGTCATAAAATGAACGAGGAAATATTTGGTAAATAATTTTATCTTCTAATTTTATTGTTTTCATATCTCTCCTATTTTTTAATTAATATATATGAACAATGCCCCAATATTTCTTTCGTATTAAAGATGCCTATTTTTGAGCAAAATAAAACTTTGTATTTTTTTAAATCAATTGTTTTATAGTTAGATTGGTTAAAATTGTGAATGACTCTTACTTCTTCTTTTTCATTAAATTTAATTGAATAATCGACAACATTTTGATCTTTAATGTCTCAATTAAATTTCAAGTATTTGTTGATTTCTTCACTTGAATTTAGTCTGAAATATTTAGTACTTTGTCTAAATTTCAAGAGTCTAATAAATCAACGATAAAGCTCTTTTTTAATTCAAGGATTTTCCAAATTATTTCATTTTAGTCCGTTAGTATAATCTGTCGTTTTATAAGAATTAAAATGGTATTTATTTTCATCTGAATTTAATTCAAAATAATCTTTGGCATATGAATAAAATGACTTATGAAAATCTTGACCACTTTTATCAGCAGGTTTACTTTGAGCTAATTCAGTACCTGCTAAAAAGAATTGACGTCCTTGTGTTGTAATTTGCAAAATCAAAGCTTGGCGATATTGGTCTAATAAATCTTTTATTTTTAAATCTTTATTTAAAGATTCTGTAATAATACGATCTCATAATGTTCCACCATCATGACAAGCAACATAAGCTAAGTTATTTCCAACATGTTTAGTAAATAAATCATTTGAGTTTTTTGAATACTTAAAATCATAACTAGGTCATTTGTAATTATTAATATTGCCTATTACACATGAACGATATTTGGCAACCTTTTCATTATTTACTTTAGTAATCAATCCTGGTCCAGACCCAAATTCATCTCAAGTAATTATTGACCTAAGTGAATCATTAAAGTAACCAAATGAATGATTATTATCACTAATTCCTTTAACATAAGAATCTTTGAAATCTAAATCACTAAATTGTCAAGCTTCACCATGGATTACTAAATTAGGTTTTATTTTTCTTAAGTTTTTAAAACAATAATCAAGTGTTTCTTTATCAATAAAACTTGATAAATCAAAACGGAAACCATCAACATCATAATATTCAACAAAATGTTTTAGTGATTCATAAATTAGTTTTCTAACCATATATCTTTGACTTGCTAAAGGCGGATAAATAACAGGTTTTACTTTAGCATTATCTCGATAATAGTAACCAGGCAGAATATTATCATAAATTGAATTTATCATCATATGGTTATAAACTACATCTAAAATAACTCCGATTTTACTTTTGTGTGCTTTATCTACAAATCTTTTAAATTCTTTAATTCTATTATAAGGATCACTTGGATTAGTTGAATAAGAACCATTGATACTAAAGTAATTTAAAGGATCATATCCCCAATTGTAATTAGTAGCTCAACCTTTGCCTTCTCCTTTTTTAAGCATTTTTATATTGTTTTCATTTAATGAATAAGTTGAATGAATTGGCAAAAGCTGCACATGAGTTATATTTAAATTTTTTAAATATTTAAAAATATCATGTTTTAATGTTGCATCAAAAGTACCTAATTTTTCTTTTTCTTTTGCTTTCTTTAAACTAGTAAAATCACGTATGTGAAGCTCATAAATGATAGGGTCTACTTGACAATTAAATTCGGTTTTAAGAAGGTATTTTATTATGCCACATTTTGATTTATTATCTAGATTAACTATTGCGCCTTTTGCAATTTTATCTTCTTTTGCTTCTCAATCAAAAGGAGCTAAAGAATAAGCATAAGGATCTAGTGCAACAGTTTTAGAATTATCTGGATGAGTGATTAAAAATTGATAAAATTTGCCTTCATATTTTTTTTCAATTTTTACAACTCAAACAGTTTCTTCTTTTTCCATAGAAAAACTAATTGTTTCATTAATATCTGAATAATTTTGAAAAATTAATAGTTTTACATCTAAAGCTAAAGGCTGTCAAAATTTTATTTCAATATGATTTTTATGGAATTTTGTTCCTAAATCAGCTTTTTTATAAGCATATTTAGCATCAAAGTCCTTAAAAAATTCTTTAGTTTGTCCTTTTATATTCATAATAATTAAATTTTAAAATTATTTTAAAATAATGTTTCAGAATGCAAAATAATCTTGATGTATCAAGTGTAAAAGTCAATATAAAAAAGCATAAATAAAAAGCACTTTTTTTACAAGTACTTTATTTAATATTTTACAAATATAATTATGATTTTAATGAAAATTCTTGATGGAAAAATCTAAAGTATTTAGGATTAATATAATACTTTCCAGCTACAGAAATGCTATCTTCATCATAGCCAAAAAATGAAACAACTACCAAGCTTTCATATCCAAATTTTTTAACATTTTCATAAGATAATGAATAAACCATATTATTTAAACCATCGCGCTCAATTAATAAGTCGGTTAAAGATTTATTAAAATTAATTTCCTCATCAACTTCAATATTTTTACAGCTTACATAAATTTCAGAAGTTGCTAAAACCTCATTATCTTTATAATAAGTTTTTTCAAAAGTTCTAAAACCTTGAACAAAGAGAATTCTTTTATCTACCATTCATTGAGGTAATTCCTTGCTTTTTATTTCTTTTCCTTCTTGAGCTGTTGCTCCTAAAAGATAACTAATTGGTTTAATTAAGTTGTGAAAGTTTTCAGCAACAAAATGTCCTCTTTTTGAAATTGAATAGATAGCTCCTAATGCTTCTAATTTTTGATATGCTTTAACAACAATACTTCTTGAACAATCAAAACGCATCATTAAAGAATGCTCACTTGGCAAGATTTTGTTTGTTGGTACTTTTTGAGTTTTAATCAAATCAATTAAATAATCAATAATCACTTGTGTTTTCTTCTTTTCTTGTAAATCCATAATATAAATATTATACACACCTTTAAATTTTAAAAAAATAAAGCTATTTTTTTGTTAACTTATAAATACCACAAGCATATTTTTGCATTATTATTCCTCATGGCATATTTTTGCATTTATATTCATATAATGCTTCAAAGTGATAATTCCTGCTAATCTTTTGAAGAACATTATTTTGACATTTATGATTGTAAATAACAAACATTAAATAATTCTTATCATCATTAAGATCTTTACGGACAATAACATTTGAATAATCTTTACTGTCTACTACTAATCTTGGATCAACTAAAGTTTCTATATTAGTTAAACTTAAAAAAGAATAATCTTTTCTTAGCTTATTAATTAAAGCAATCATATTATAGATACTATTTCCTGGTGCTATTTCATTTTCAACATAATCAAGCGAATTAGATACATTTAAAAATAAGTTTGATTGACAAGCATCTAATTTCTTTTCGTAAATAGCTGATATTTCTTGATGGTTTTTTCACTTCATTGGTTCTCTTAAACCAGGATCATCAAAGCTTCTAGTTCCTGCATATGCTAATTCATCACCATAATAAATTATTGGTTTAGCAGGCAAAGCTAAAAGATAAAGCAAAGCAGCTCTAATACTGTCTTTTTCATCTTCTGTGATTTTAGGATAGTTTCTAATGTTTTTAACTTGGCGTCGATAATTGTCTAATCATCTAAAAGTATCATGATTATCTAAAAAAGGAATTCAGGGTGCTTGAAATTTTGTATTTTGGTATTTAGCAACAAGGCTTTGTAAATAAGAAAAATCTAAACGCACATCATCATTATCTCTAAAATGTTCTCATCCATCATAAGTAGAGTCTAGTGCTTTTTGATTATTATAGGTAAAATATTCAAAAGCTTTTAATGCTTCACTTAATCACTCACCCATCATAAACACTTCTTCACGATTTTGCTTTTGAGTGATTTCCTTGCTTGCTTGTCTTAAAAGATGAAATATTTTTGCTTCATTAAAATTGGTTTTAGTTTCGCTTTCAGAAGAAAAGTATTCACTAAAGGCATCATATCTAAAACCATCAACACCTAATTTAGTTCAAAATTTTTGAATAGCACAAAGTTCATTAATTACATCTGAATTATTTAAGTTTAAATCAGGCATACCGCCTCAAAAGCGACCAATAAATTTTTTATTTGTAGCTTTAAAATTTTGATCAATGTTTTTATATCTTTTTCTTAATTTTTCATTATCAACTCTTGCATCTAAATCATTTTTAAGTGGTTTGAAGTTATAATAATTTTCATATTTTTTGTCACCTTTTAAAGCTGCTTGAAATCAAGGATGTTCATAAGAAGTGTGATTAAAAACTAAATCTAAATAGATTCTTATACTTTTTGAATGAGCTAATTTTAGGAATTTTTTAAATGCTTCCATGCCGCCTAATTGAGGTGCTACATCACAGTAATCAATAACTGAATATCCATGATAACTTGATGAAGGATGAATTGGACTAAGTCATATTTGGTCAATACCTAAATTTACCAAATAATCTATTTTATTGATTAATCCGTAAAAATCACCAATGCCATCATTATTACCATCCGCAAAATTGTAAACCAAAATTTGATAAATAACATTGCTGCTTAATTTTGATTTATTAATACTGCAAGTTTCAATTTTTTGATCTAAATTTAAGTAAGTTAAATTTGGATTTTTTATTAAATATTTTGCATCATCTCAAGATACATAATTTTGGTTTGGATGTTTTTTAATGAATTTTTTAAGTTTCATTTTTTTAATAAAGTTCATAAATTTTTCTTTTAATTTGTTTGTTTTCATAATAATAATTTTAACTTTTTTAAATATTAAAATATTTTTTAAATAAAAATCTTGATGTATCAAAAAAGATGCCGAAGCACCTTTTTAATCATTATATTAATTGTTTTTCTAAGTCCTTAGTATCAATTTTGCTTTTAGCAATAATTTGGTCATTTTTAACATATCCATATTTTGTTTTAGCAACAATTAACAAAATAAAGTTTGTTAGTATAACGAACATTCCAATTCATGCATAAGTAATGTTCATTTTATCTAAAAATTGATATATAGCATATTCTGAACCGCTTAATTCTTTAAAGTCAATAACTTTGTTAAGTTTATTAGGACTTAGTGAGATTGCAAAAAAGAATAAACAAAAAGAGGTTAATTGGAGAAATATAAGATAAATAAAGTATTTGTCTGCATTACTTTTATTTGAGAAAAGTCGAATTACTGCAATACAATTAATGATTAAAGATATGAAGAGAATATTTATATTAATCATTATTAATGCAGGAAGGAAATTTTTAAGACCATAAGAAGGAGAGTTTGCAAAAGAACTATTGTAAATCTTTTCTTTAGTTGACTTGATAGCAGCAGGATCTACATCTCGAAGTCAATTTAATTCTTCATCTATTCTTTTTAAGAATAAAAAGAGAAAAGCAAACAATATTGCTATTGCAACTATTGTAATAATAATTGATAATACTAATAAAGATTTACGAGCTAAATAATTTGTTTTAGATTTTTTTAATTTTTGGTTAACTTTGAACATTAATTAATCCGTTTTGTTGTTTCCGCATCGAAGATATGGATTCTAGGTTCATAAAATTCAATGTAAACTTTTGAATAAAGCTCGTAAGTATCTGAATTGCTTGCTGTAATTGTAAGTTCGATATCGTCATTATCCAATTTAACAGTTACAAGTTGTTCTTTACCAATTAATTCAATATTTTTAATAGTTGCATGAATTGAATTTGATACTTCAGAAGCATGAATTTTGATGTCTTCACTTCTAAAACCAATTTTTAATTTTTTGTCTTTTAATTGTTCCAAATATTCTTTTGGGGCTTTAATTTTAATGTCTTTTTGAGCACTAACAATTTTGCCGTTTTCGCATGCTGCTTTGAAAACATTCATGGTTGGAGAACCAATAAATCTAGCTACAAAGACATTAGCAGGTTTGAAGTATAAGTCACGGCCAGTACCATTTTGTTGAATTGAGCCATCATTGAAGACAACAATTTGATCACCCATTGTCATAGCTTCAAGTTGGTCATGAGTTACATAGATACTGGTTGCGCCAAGCATTCTGTGGATATTAACAATTTCACGACGCATACTTTCACGAAGTTTTGCATCAAGGTTAGAAAGAGGCTCATCCATCAAGAACACTTGAGGTTTACGAGCAATAGCACGACCTATTGCCACACGTTGTCTTTGACCACCAGATAAGTCACGTGGTTTTCTGTATAAGTAATCTTCTATTTTTAGAATTTTAGCAACATCCTTAACACGTTTGTCAATAACGTCTTTTCTTTCTTTGGCTATTTTTAAACCAAAAGCAATATTATTGTAAACGTTCATGTGAGGATATAAAGCATATGATTGAAAAACCATTGCAATATTTCTTTCACTAGGTAAAAGGTTATTATATCTTTTGCCATTGAATAATAAGTCACCTTTTGTAATTGAATTTAATCCCGCAATCATTCTAAGTAGTGTAGTTTTACCGCAACCACTAGGTCCTAAAAAGATACAAAATTTACCTGGTTGAATATCTAAATTAATATTTTCAAGTGTATAATTTTCATTACCTTCATATTTTTTAGATAATCCTTTTAGTTCAATATGAGCTCCTTTTTCAATATTTCTAAGCTCACCGATTTCATCTAGCATTTTGTCCAAATTAATTGTTTCAAAGGATAAATCGTCAAAATCTTCATTTTGATTTTGAGTATTATTCAAGTCTTTATTTTTCTTTTTAAATCACATATTTTATCCTTTCACAGCTCCTTCAGATAAGCCACCAACAATGTATTTTTGTAAGTACATAAATAGACAAAAGGCAGGAAGAGAAGCTAGTATTGAGCCAGCTGCATAAGCACCAGCATTTTTATGTTTTTCTTCAGCATTAAGTAAAATATCTAATCCAACAGCTAAAGTTTTTTCTTCGTTACTTATTAAAACAAATTTCGGTAAGATAACATCCGTAAATGGAGTTAAGAATGATCATAGAGCAGTCATAATTAAAGCAGGCATAATAACTGGTAGAAGTATTTTGAAGAATAATCCTCAGTTAGAACATCCATCAACTCTAGCTGAGTCATCTAATTCTCTTGAAACTGTATCTAAATAACTTTTTAGCATAAAGGTATTTGAAGATATAGCTCCACCAGAATAAATAATAACCAGCATTAATATTGAATTAATTCCTAAATCATTCCCCATCCGCACAATAATGTAAAGACAAATAAGAGATGAAGTGGCAGGAATCATTTGCAACATCATAATAATAGTTAATGAGTGTTTTGAACCAGCGAATTTAAATCTTGAATAAGCATAAGCATTCAAAGCAACACAAATGGTTGAGATAACCATAGTTAAGAATGCTAGCAATAATGTATTACCATATCATCTTAAAAAATAGCTTCGTTCACTTGTAAATAAATAAGTAAAGTTGTCAAAGCCAAATTTAAAAGGTGTTATGGCTACAACCCTTTGGTTGTTAACATTTAGTGAGGCCACAATTAGTGAAACCGCAGGGAAAAGCACAATCAAGGATCAACAAATTAAGATTAAGTAATTAAAGAACAGTCAAACTATTTCCATTGGTGTTGGAGGTTTAGTATCTGATAAGTTATAGTTGATACGAACTTTTTTGTTGATAACTTTTTTAGTTGAAATGTTATCAACAATTGAATCATAAATTTTGTTTTCACTATTCATTATTAGTCCCTCCTTGTCATAGATTTAATGAATCCTCTAGCTGCTACAGCGATACTTAATAGAGCTGCAAGAGTTGTCAAAGCTGCTGCAAAAGCTTGATCAGATTCAATTTTTACTGCACCAGTGGCTAGTTTATAGATAAATGAAATTAGAATATCAGTTGCACCTTCTTGATAAGCAGTTGGTTTAGCAAACGCAGGGCCTCCGCCACTAAAGATACTAATAGTTGTGAAGTTGTTGAATGCTCCAACAAATTGACCAATTAACATTGGGGCAATTGCTAATAATAAAGAAGGTAGTGTTAGCTTAAAGAATTTATGATAATTTTTGGCTCCATCAATAGAAGCAGCTTCATAAATATCTTTTGGAATTGCTTGTAAGTTACCAGTAACTAACATGAAGATAAAGGCATAAGCTATTCAAGTTTGAACAACAATTAAGATAGCTCGAGCTGAGGTTATGTTGTTAAGTCATTCAATAGGTCTATTAACAAAATGAAGTTTTAATAGAATCATATTAATATAACCTGTTGTTCCATTAACAAAAGCACTTCTTAAGAAGTACAACATAACGAAAGCTGGAATTGCTCAAGGAATAATAAAGATTAATCTAAAGAATTTTCTTCCTTTAAGACGATTATTATTTGCTAATATCGCTAAAATAATACCGAGTGAAATAGGACAAAGAGTTGAAGCGATTGTTCAAATAAATGTTCAACCAATAATTCTTTCTAGAGCTGTTAGAATTCCATTATTACGATAAATTCATCAAAGTCCTCATTGTTTCAAGCCAACTCATGAACTGGTTTGAGTAGGGGCTTGATGCATGTATCCATAGTCAGTAAATGAAATTAGAACTGAAGTAATAATAGGAGCTAGAACAATAAATAACATTAAGATTCAACCTGTAATTGAAATCATTCAAGGGAAGCCGCTTGTATTTAATCATCTTTTTGTATATGATCATTTACTTGGACGTGACCCTTCTTCCATTAATTTAGCTGTTCTAAATGCAATAATTGAACAAACTGTAAAGTAAATTAATACTATTGTTAAGATAATAACAGATATGACACCACCAAAAAGGTAACGCCGAGCATCTCTAAATATGCCCATGCTAGAATTGAAGTGTCGAGCTCCTAAGTCAATGAAACTCAGAATCCCTTCCATACCATCAATACCAGTAGTATAAGCTCCGAAACTAAAAGGAATAAAAATTGCATACAATAATGTGGTTACTAAAAGTAAAAGAATCCCTTTAGTTTTTTGTTTGAAGAATATCAATTCTGAAAGTCCAGGGAAAATAAAACTAATTATTGCTGCTAATCATTTAATATTACGTGTACATTCAATTGGTACAGTTTTAACAGCTTCATTAACTTTACTTTCATAAATTTTTGAAACAACCTTCATTTCGGCTTGTCTTCTAAAGAATCAAGTTGATAAAATTTCTTTATTTTTAAGACTGTTAGTTTGAAGTTTTAATTCATATCTTTTTTCATTATAATCAATCTTTGCTTCCATTTTACTATGTTTAAAAGCTTGTTTTGATATTTCTTTATTTTTATATCTTAAAGCTGTTTGCTTAACTGTTTCAAGATATTGATTATAAAGTTGTTTTTTCTTGTTAGAAACTAATTCTTTTTCGCTTTGATATTGTTCTTTGTTTCTAACTTGTTCTTTTAAAATGTTAGAATTTTCATTTGAGCTAAGATTTAAAGAATTTAGTTTTGTTTCATAAATAGCTAAAACTTTTTCATATAAGAATTTTAGTCTTACTTGCTCAGCATTTATTGCTCTTTCTTTTAAGGCTAAAGCTAATTCTTTTTTATAATTTCCTTCATAAGAATATTTTTCATAAATTGCCGATTCTTTGAAATAAAGTTCAGATTTAAGCAATCATTCAAATTCTTTTTTTAGGCTTTTTGTGTTAAAAAAACCAAACAATAATTTTTTAAATATTTTAGCTAAAGCAGATGTTTTTTTGTCATTATAGACAAGTTGTTTATTTTTCTTAAATGACTGTAAAACAGATTTTTTGGTAATTTTTATAGATTCATCTTTATTAGAATCTATAAAGTTATTTTTAGCTAAAAATTCTAAATATTGTTGGTAATTTCTAGTAGCAACTAAATCATCGATTTTCTTTAGTTGTTGCTTGAAATTTTGATTTACAATCTTAATTTTTGATTTTCCATTTTGCTTTATTTTTTTAATTTTTTCAATATTGCCTTTTTCAACACGTTTAATCTCATTTTTGTGTTCTTCGATTTTAGCATATGCTTCTTTTTTATATGCTTTTACTTCTTGTTTTTGTAATTCTGCTTTTTGATTATATTCATATTCTAAAGCAAGATTTTTTTGATTTGTTTCTAATTTAATGTTGTATTTGTTTGCTTTATAAATCTTTAATTCAATAAATTTATTATTTTGATATTTGTGGTTTAGTGAATTACGTTTTTCAATTAATTCTAAACGACGTTTTTCCAATTTTTGATAAAAGTTTTGGAAATAAGATTTTGGGTCTTCTAAAGTATTTAATATTTTTAGTTCTTGTTTATGCAAATAGTTTTTAATTTTATTGATGTCAAAATCAAGATCTGAATTATGTTTAAGATATTTTAGAGTTATATTGAATAAAGCATATTTAGAAAGAATTTTATTTTCTTCTTTAATTGTTTTTTGCTTTAATTCTTCTAATAATTCATTCTTCTTTTCATCAATGTCAGCTGTTAGTCTTAGTGATTTTTGAAATTCTAGTACATATTTTTTAAGAGCCTTTTTTTCTTTTATTAACGCTTTAATTTCATACTTAATCATTGAGATTTCGTTATCAGCAAACGATATCTTTTTAATAGCATCTTTAATAGCTTTAATTTTATTTGAATAAGCCGCATATAAACTTGAAAGTTCTCTTTTTAAGTTATCTTGTAGTTTTGTTCTAGCTCGTAAAAATAAATTTTTGTTAATCTTCATTTGTGAATTAATTTTATCGATACGCCTATTAACAACATTTCTAACATGATGTTTATAGGCTTTTAAGGTATCTTGTTCTTCAGAAAGAATTGTGTCAAATTCTTCTCCATATCAGTTAAAAAGTTTTAATTTTTCCATATTAAATGTCCTTTATTCAATGTGTACCATTCATAATGGTGCATTCTTCGAGCTAAATGTAATTTGACTGTTAAAAAGATTAGATAAGAAAGTATTATTACACAAAAGATTGTAATAAATACTCCTATAATAATTTCTTGTTTTAATAAAACTCTAGTGCCTCTAAATCAAGGCAAAGCTCCTAAAAGAGCAAATACTAAAATTCATATAATTAAAAACCCATAGCCTGCTTTTAATTTATGCCTTAATAGAACAATGTAAATTAGAAAGCAAATTAATGCTAATGAGTTTGCAATATAGGAAACAATTTTAGTTATTGTCACTTGTTTAGCAATTTGTGCTTTTTCTTCATCGCTAAGACTTGTCTCTATAAATTTAGAACCATAAACAAAAGGAATTAAGACTCAAAAAATCATTAAGACTGTAATTGCTGCTAAACAAGAAACTAAAAAGTAAAAACTAAATTTGTCTATATTTAATTTGGGTTTAATGTATGGATTTTGATCCATTATTCAAATCTAATAACAGCTGTCGATTCAGTTGAACTATTAATTGTGTCAATTTTTTCTTTGTAAATTGCTGTTAATTTTTCATACATCACTTTTTTAAATGCGTCAAGTGTTTTAGTTGTTGTTGCAATTTCAGAATATAATAATTTGTTTCCAAATACAGCATCATTTCATGAACTTCATGCAACATTACCAAATTGAGGATTTGATTGGTCAATACGTCCTGGTTGGTATTGAATTACATTCAATCCACCATCAGCTAATGATTTAGTTGAAGATAATGTTTGATAAATTAAGTCAACTGTTTGATTTTTTCTATATTGTTCAACAATTTCAATAACTTTAGAAGCTGGCACTTTAGTGCTTTTATCTTTTGCTGGAAATTGAAATTCTTTAGTTAATTTGTCACCAAGATTTCTAACTTCAGCTTGGTTAATGTATCTTGCGTAAAGATATGCTTTAACTGCTTTTGTAATAACTTCATCAGCTACTGCTTTTTTAGATAAAAGTTGTGAAACTTTTTTAGCTTCAGCAATTTTTGCATTGATTAAATCTTGCAATTTGTTTTCTTTTAATGCTGTTTTGAATTTTTCGACAACAGCATCGTATTCTTTACCATTGTCACCATCAGCACCAAAAATGATTTTTTCAAGCTTTCTAATATGGAACGAATTGTCACCTTGTTTTGCATTTGCAAATAATTCATTTAAAACATTTGCTTCGTTTAATTTTGTAAAAGCTACTTGTCAAGTTTGGCCATTACCTTGTAAATCCGAAAGTTTTTCCAAACCTAAAATTGCTGCTAATGCATTACGTAATCCAGTACCTTTTTCAGTATATTTATCACGAATATCTTTGAAATTCATGCCTAATTTAGATTCTAAAGTACTGTTTAACACAGCATGTACTGGTAAATTGTTAGCTGAATACATATTTGAATCCGCAAGTGGGTCATCATTCGCTGTATTTTCTCAAGTACTTGCACTTGGAGAATCGCCTACATTTGATAAATTATTAATTACTTGAATTGATGAATCATAATCTCTATTAAATTCAGCAGCATCTGTATATTTCAATTTGCTCATTAATTCTTGTAATTTTGCATTTTGAGCGGCTGAATCTTGATCAACTTTTGTTTTTAATTCTTTTTGTAAGTCTACAATAAATGGAACTTTTGAATCGCTCTTGAAGTATTCATAGAATGATTGAGAATCAAATACACATTCAAGAAATTCTGCAATAGCTTCTTTTCTTGCTTTTGATTTACCATTATTTCTAATGTTTACTAAATATGATCAAGAACTTGAAGCTTGACGGTATGTATAAGCGTCATTTAATTTGCCTGCATTAAAGAATGTTTTGGCATTACCTGATTTTTGAGCTACTTGAACATCTCAAGTACCAATTAAACCATATTCTAGTTTGCCTTCTTTCATTAATTCAAATACTTTGTTATTTACAGCGCCCATGTCATTACTTAATAATGCTTTTAAGTCATCTTGTTTAATATTTCTTTTGCCTCATACTGAAGTAGCATATGCTCCTGAGTCTAGGATATATGCAGCTTCATATATAGGAAAGAAGAATTCTGACATTAAATCAACTGCTTTTTTGAATTCAACATGGATAGGATTTTCTGTGTAAAGACCTGTTGAAATTTTTCCATTTTTTAAGAAAAGTAATTTCTTCATCATATCTTCAGCTTTGTATTCATGAGCTACAGCGCCTAAAATACCATTACCATATCAGAAGTCTTGGAATCTTAAAAATGCACGACCTTGTTTTACTAATTTTAGCAATGTATCATTTTCAGGTTTAGCTAAGTCTTTTTTAACATCTGCTAAAGATTTTGTTGAAGCAACTATAACACCTTCAGTGTTGTGTCTAATACCAAAAAGTTTTTCAGCTGGTAATTGAGTAATTTTTTCAATACCGACTACTGAACCAAATGATCTAAGTTGTTTCTTTTCTTCTTCAGTTGCTTTAACTTTTTTAGCAATACGATCTAATAATCCTGGTGAAAATTGATCTAAATCAGCAACTGCATTAGATGCAACTAAAGTTGTAATTCTATCATGTGGTGCATAAAAAATATCTGGCACAATTTTTTCATCTGTTGCCCCTGTAGGCATTTCTAAAGCACCTCAAACATCTTTAGCTATTGTTTTAATTTCATAGCCATTTTGGCCTGATTTTGTTTTCTTAAACAATTCAATTGCTTTGTCATAGAATTTTTGTTGAACGCCATCAACTGCAATTAAAATTGTTTTGCTTTCAGTAGTACCACAACTAATTGTAGCTAATGCTGTCACTGGAGCTGCTAAGGTTGCAAAGCCAATTGCTATTTTTTTAAAAGTTTTCTTAAAATTACTCATAATTTCTCCTTGTTAGCTAACTATTCAAAAACGATTAGATTTTTTTCATAAGCAACTATTGAAAAGATAATTGCTAGTGGACTAAAAAAAGTTAAGACAAATATTGTTGCAATAATTCCGCTTCATTTGTAAAATTCGGTTTTATCCAAAATATAGTAAACATGAATGCAAACGAATATTGCAAATATATAAGAAAGTAATGCAGCTGAAAATAAAATTCTATAAGTATAACTTTTTATTTCGCTAACTATGTTTTCTAAATATTTGCCTTCAAAAGTTCATTTATTTTTGCCTAGTAAATACAAAATTAATATTTCTAAAATCAAAATAATGATTTGAGTAAAAACTAATAACATGTATTCAAGAATTTTAGGTAATGATCTTCTTTCATTTGGTCATTTGCCTAAAAAAAGTCATCTCATTCTAGTTTTAAATGAAACTTTTGTACTTTTTTTGTTTTCTAATCTCCTGTTTTTTTGCATAATTAACTAAATAAATTATATTTTAAAATTAAAATATATGCCTTGTCAGTGTGCAATTCTTGATTAAATATAGGAACATTACCCTTGACCACTCAAGATAATAAAAAATTCAAAAAACAACAAATTTTAGTGTAAAAAATTTCTTTTTGCTGTTTTTTGTGCATTTTATATATTCTTATTCTTAATCTAACTTAATCTAATTGTGGAAATTTTCTCATATATGAGATTTTAATTTTTAATAAGTTAATTCAACTAACATTAAACCAGTATTCCCACTAAAAGTTATTTTTTCATTGTTTATCGAAGAATTATTAGAAAAAATAACATTAATTTTAGTTGGAGCATTCTTAAAGACTAGATTTCATTCTTGACCATAAGCTGTATGATAATAAATTGAATTGACTAAAACTAAAAACTTTTTATCTTTTTGCTTTGCTTTAACTAATTTATATCCAAGTCCTTTATAATCATAACCGGGTTTAAAGAGATCATCTAAATTAGCATAAGTATTTTCTTTTTCAGCAAACATTTTTGGATTTTCTTTACGATAATTAATTATTTTTTTGGCTAAATTAAAATTAGATTTTTTATTCTTATGAGCTTGTTCAATCGAATTCTCATTCTTGGATAAATTCAAAAAGATATGACTAGTTTCAATACCCGAACGTCTTTCATAAAAATCTACTTGGTTTTTTTATTACTTCATTTAAAAGCTTCTCTTAAAAAGGCATCAGAATGTGCTTGTGGGCCACCTCTCATATTTAATTCATTACCATTATAAAGAATTGGATTGCCACTTTGAATTAATAGGTTACTTAATCCATAACTCATTACATTTAAAACTTTAAAATCATTTTGCTTAATTTCGAGATAAAAAGTTTTAACTCCAAAAATCAATTGGTTATTATAGTAGCGCTTGTTAATTCATCTATCTTCATCATGGTTATCTAAGAATGGTAATCATGTTCTAATATGACCTTCAACTTCATGTTCTTTTAAAATTTTTTCATAATCTTTTTTTGAAGTTTCAATAAAAAAACCATTTTTAAAATGAGAACCATCAATTACAATATTTAAGCCAATTCTTTCTTCATAAGGTTTTGTTGTATCATTAATTGCAAAATATTTATGAGCTTTACTTGGTTTATTTCATCATTCACCAAAGAAAAATATATCATCGCTTGATCTTTGATCTTTAGTTTCTTTTATTACTTCTCTTAAGGCTAATCTTTGCTCATTAAATAATTTTCTCTTTTCTAAGCCTGTTGCGTCTTTAAATTCATGTTTATTTTCTGAATTCATAATGTAATAAAAAGCATCAAATCTAAAACCGTCAACTCCTTTTTTAACTCAGAATTTCATAGCATTAATAATTTCTTTTCTTGTTTTGGAATTCTTTAAATTTAAATCAGGCATACCGCCTCAAAATTCAGCAACATATCTTTTATATGAAGACTTTTGATTAGAATCTATATTGTAAAATAAATTTCTTGTACTAGAATCATCAACACCATATTTCTTTTCACAATTATATTGGAAGTTGTAAAAATTATAAAATTTGTCAAATTCGTCTTTGTTTTGAAGACCTGCTTGGAATCATGGATGCTCAAATGAAGTATGATTAATGACTCAATCCATAATAACTTTTATGCCTTTTTTATGGGCATTTATTAAAAATTGATCAAAGGCTTCCATGCCACCTAATTCAGGAGCAACATCTGTATAATCAATTACATCATAGCCATGATATGAGCTGGCTGGATGTATTGGTGAAAGATATAAAGTATCAATTCCTAAATTGCTAAAATATTCTAAATTTTCATTTAGTCCAATAAAGTCGCCAATACCATCATTATTGCGATCTGCAAAAGAATAAATTGTTAATTGATACATAACATTTGAAGGCTTAATAGATTTGTTAAAAGGCACAATAAATTTTGCATTTTTAAATTCACCTTCATAAGACAAATTCTTTATATTTTTTCAACAAATTTTTCATTGTTTCACAATTAATAATCTTTTTTAACACTTGTGCAACTTGCACTTAAACATAAAGCGATAGGTGCAATTAAAGAAAAATATGCTAATTTTTTTCTTGTTTTTTTACTCATTTATACCTTCTTTAATACAAATATTATATTAAAATAAATTATCTTAATAGTACAAGCTTGCAAATCAAATAAAGAAAAATTACCAAAATGCTTATAAAACAATGTTTTTAGTAATTTTAAGTTATTTTAAAAACTTAATTCAACTAACATTAATCCTGTATCACTATTGAAAATAATTAAATATTTTCTATTTTAGAATTATTAGCTGCAATTACATTAATATTAGTTGGTGTGTTTTTAAATTTAGTTTCTAATTGTCAAAATTGTTTGTATGATTTTTTATTGTGTTAGCTAAAACAAGTATTTTATTGACTGCTTTTTTATCTTTAAGATAGTATTCTGTATCATTGCCATAGTTTCTAAAATTATCTTTAAATATGCCATCTAATTTGAATTTTGGATAAACATATTTTGTTAATTCAAAAGCAAGACCATAGGTTTTAGAGATCTCACTTTTGAATTAACAAAATAAATATAAAATTTCCAGCTTGCGCTGGATTTTGCCAGCTTACGCTGGAGTATTTTCTTTTGCATTTTTTCACTTTCATATGAAAAAAATGATTTTTAAATTAAAATTTAAGCAGACAATTTAAGAAAATGCATTTTTCTTAAAACTTGCCGCCCTCACTTTTGGAAATAATAAAGTAATAAATTTTGATTCTACTTTTATTATATTAATATATAAATTAATATAATTTATTGCTTGATATATCAATGCCAAAAGCAAATAAATAAAAAAACTAAAAAGCCTGTTTTATAGACTTTTTAGTAAATTTTGAGTAAAAATAATTAATATGAAATTTTGCTATTTTTTTCCACAAATTTTTCTTTTAATAAAATGGCCACAGAAAAAATTGGATTCATTATTATTGAGTAAATTGGGATAGTAATAAGGCTTTTTAAAAGGATTGGAATAGTGATTAAGTAATAATGCTCACCAGTATAATTTAATGACTTTCTGCCAGCATAAAGATAATTGTAATAATTAATGTAAACATAAGGCCCAATTAGTCATCTAAAAATGATCATAATAAAAATAATAATACTGAAGACAAATAGATAATCAAATCATCTTTTTTTCACTTTTTTATTCTTAGCAAAAAGCAATTGAAAAATAATAAAAATAAATGCGATAACACTGAAATTAAAATATATTAAGAGCATTCATAAAACTGCATTTTTAGGTAAATATTTTAGCTTCTTAATTCCTTTAATATGATAACCTTTATCATTTCCACTATTTATAACAACATAGAGTAATAAAGCGAAACCAACTGAAATTACAATTATTGGAAAAATAATTCTAAATAAAAAACTAGCACGATAAAAAAAGTAATAAAAGGAGCTTAAAATACAAATGCCTATTGGAATCAATGCATAAAGATAATACCAAGTTCCAATTGTACCAGTTAAAAGCATCATAAAAGTATCAGCTAAAAATGATATTAAAGATCCTTTAAGTGGTCCAAAAAAGATTCCATATAATATAAAAAAAATTGCATCAGTTTCTAAGGGTATTATTTTTAAAATTGTAAACTTAGAAATTATTGCAACAACAAAATGAATTGCCAATAATATACCTGCTAAAGCAATATCTAAAACACTAAGACGAAATGACTTAGCGAAGTGTTGCTTAAACTTTTTGCCAAATGATAATTCATTAAAAGATTTGTCATCTTCATTGGCTGCAATATTATTGGTAAAATTCTCATTTTTTTGCATATTTTGAATTATATGTTAAAAATTTTTATTTGTTATTATATTATATATATTATGAACAAAAAATTAGAATTTGGAACAGCTGGAATTAGAGGAATTTTAGGTTCTGGTGAAGACCATTTAAATGTAGCTCATGTTCAAAGAGTTATTCATGGTTTTGCTAAATATTTAAAAAACAAATATAGCCACATTAATCCAATTACTGTTGTTATTGGACGTGATAACCGTAGATTCTCAGCTACATTTGCAACCTTAAGTGCTCAAATTTTAAGCCAATATGACATTAAGGTTATTTTTCCTCTATACTTGACTCCAACACCTTTTGTTAGTTTTGCAATTTTAAGAGAACATGCTCAAGGTGGAATTAACATTACAGCAAGTCATAATCCAGCAGAATATAATGGAATAAAACTTTACAACGACATGGGAAGTCAATGCTTACCTGAAGAAATTAAAGAAATTAGTGCTTATTTTGAAGATTATTCAAAATATGATGAAAATCTTAAAGTTCCTAAAAAAGAAGCTATTTTTAAAGAACTGAAAAACCATAAGTTTTATCCAAAGAAACTTTACAATGAATATATCGATAGGATTCTTAAATTAGGAGGACTTGTTGACTTATCTGATATTTCGTTAATCTATTCTCCATTACACGGGACAGGACGTGATTTTGTATCTGATATATTTAATAAATTTATTGAAAGATACAGATTAAAACCTGAAAATGTTCATTATGTCAAAAGTCAAATGTATCCAAGTACATCTTTTAGACATTGCAAATATCCAAACCCTGAAAGAGAAAAAACATACAGCAAATCTATTAAACAAGCTCAAAAACATAACGCAGACATTATATTAGTAACTGATCCAGACAGCGATAGAGTCGGTTTAGTTGTTAGACATAATAACGAATATGTGAGATTAAATGGTAATGAAACAGCAACAATTATTTTTGATTACTTGATTAGTAAATCAAGTATAAGTAATTTTGCAAATCAATACATGGTTTACTCATATGTTTCATCAAATATGCCAGCAATTTTAGCTAGAAAAAATGGTATAAAAGTTTATGAAGTTCCAACAGGATTTAAATGAATTGGAATGATAATAAATGAAGAAATCAAAAAAGATCAACACTGCATGTTTGCTTTTGAAGAAAGCTATGGTTCATTAATTGATGAAAGTTTAGCTAGAGATAAAGATGCTATTCAATCAGTTGCTATTTTAGTTAAAATAGCTGCAGAATACAAAAAAGAAAACAAAACAATTGTTGATGCTTTAAATGAAATATATAAAGAAAATGGTTACATTGTTAGTGGTAATGTTGAAATTATGACTGATGAAAATACATCATTAAGTAATATTCAAAACAGTTTTATTAACTTAGATTTAGACAATAAAATTGTTGATGACTTTAACAAAAAAACTGATTTTACTAAATCAAATATGATTAAAATTACATTCTCAAATGACGATTCTTGACTTGCACTAAGACCATCAGGAACAGAACCAAAAATCAAATTTTACATTTTTGCATTTGGTAAAGATCAACAAGAAGCTCAAGCAAAATTTGATAATTTTGCAAGAATTATAAAAGAAATAAAATAACCATAATATTAAAGCAAAAGGATTAGCCTTTTGTTTTATTTAACTGTTTTATAGGCTTATACAAAAAATGAACTTTTTAAAAAGTCCATTAATTGATTATTTTTCGTCGACATATTTAGTAATAAAGCTACCTGAAATTCCAATTACTGGTTTAACTGAAATTCATAATTTAGTATCTAAAGTTTTTAAATCACGAATAATGCTACGTGTTTCTAAAAGAAGCATTGTAGTTACTACACTATATGTTTTTAAGCCTGAGTAACCACTTATGCCTTCAACAACAGTGTAGCCATGTCAATAGTTAATTTTCTTAAAGTACGCTAACACTAAATCTGGTGAAGGCCCACAAGAAATTTCAACTGCAACTTTTTTATATTTAGGATAAATAAAGTTAATTAAGAAGTTAACAACCAATATATAGAAGAATGTTGTAAATTCTCTCATTCCGAAGAATACTCTGTTACCTGAATCTTCAATAATTTGAGTTCCAACACCATTTGTTAAAATTTTTGCATAGTTATGATGTGGTTGAGCAAAACCAAAAATGATTAAGAATGTTATAGTCATTATGATTGAAATAAGACTTAAGATATTGCCAACACTTTTTTGTTTTTTAGTTGAGAAGTAATAAGCAACAATATCTGTGCCGCCAGTTGATCCGCCATTTTTTCAAGCAATAGCAATTGCTGTTCCAGCACAACCTGAACCAATAGCTCCATAAGCAAAAATTGGTCAAGAATTAGGATTTTCTAAAGTTACACTTCCAACTTTTATTTCTTTAGTTCAACCAGGTGCAAAATTAATATGTTCATGTAATCAGTCTAAAACTATTTTATTTTCTCCAATTGGAACTGAAGTTAATATCAAATTAGTTAATATTTGAAATAGCATAAAAGCTAGTGTCATCAAAGTAAAACTTTTCTTAATCTTAAATCCAAATCCTAAAAGAAGCGGTAAGTTAAAAGCTAAGTAAATTAAAGCAAAATAAGGCTTAGTACTCGGTGCAATTAAGTTAATTAATAAGGGAGCTCCACTAAGTCCACTAGGAATAGTTTCACCTCTATTTAAGAATACTTGCACACCAAAGTTAAAGACCAATGCTGATAAAAAGATAAAAACTAAACGACGAGCATAGAGTTTAGCAATTCTTTTCCTAACTTCCTTGTTTTTAGGATTATCTTTTTCATAATTATGACGGACCATTTCAAGACTGTCAAGAGATTTTTCATCAAGAATTTCCTTTTTAGGTTCTTGGATTTGATTTTTTGCTTCAAAAACCATATCTTTAACTTCTTGAATAGCTTCTTTACTAACTTCAAGGTTATGCACTTGTTCTAGTTTGGTAGTATCTTTTTCGTTGTTATTCATAATATTAATTTTATATTAAATATTTATTTTTTTAATTAATATTTTTGTTATATAATAACCATGTTACTTTTGTAACCATTCTGAACAGGTTTAAGAGCTTATAAGCCACCTTAAAGATGAGCCACAAAATTCAGGAGGTATAGCATGATTGCTATTATCGAAACTGGAGGCAAACAAATTTTAGTTAAAGAAGGCCAAACCATCTTTATTGAAAAAATTGAAGGTAAAGAAGGTTCAACCGTTGACTTTGACAAGGTTTTATTAATCGACAATAAGATTGGTAAACCATATCTTAAAGGAGCCAAAGTTACTGGAAAAATCCAAAAACAAGGCAAAGCAAAAAAAATAGTTGTATATCGTCACAACCCTAAATCAACTCATAAGAGAAAATTGGGACACCGTCAACCTTACACACGTGTAGAAATTACAAAAATTGTAGGATAGGATTATAACATGGCACATACGAAAGCCGGTGGTTCAACCCGTAATGGTCGTGACTCTCGTGGTCAAAGACTTGGAATCAAATTAGGTGACGGACAATTTTGTACAGCTGGATCAATTATCTTTAGACAACGTGGTACAAAGATTTTCCCAGGTACAAATTGTGGACGTGGAAATGACGATACATTATTTGCTTTAATTACAGGATATGTAAAATTTGAAACAAGAAGAAATAGAACATATGCCTCAGTATATCCAGAAAGAGTTTCAAGTGCTCTTTCACAACCAGTTGTAAAAAAGGCTGCTGTTAAAAAAGAAACCGCTCCTAAAGTAGCGGTTAAAAAACCAGCTGCAAAAAAAGCAACTACAACAACTAAAAAAGTTAGTGCTGCAAAAAAAGCAACTAAATAATTTAGATAAATATGCACATTTTTATGATGTGCATATTTTATTTATTTGAGTGTTTTATAGGTTTTTAAGAAAAAACAGGACTTGCCTGTTTTAACTTTCTTTAGCAAATTTTCTCTTTTGAAAAATCATTGCAATAATTGAAATAACAATTGAACAACCTGCGACAATAATTGTTAAAATGCTGTTTCAAGTTATAAGTTTTCTCAATAAGTCAACTGACTCTTCTACGCTTAAAGAAGAATTAGTTATTTTTGAATATACAAGTCCATATAAACAACTTATTGTAATACCTGCTATTGTAGCTGCTGCTAAAATGATTAAGATTGTTCCAAAGACTCAATTCTTTCTTGCTATATGAGCTATTGATGTTACAAAGTAAACAATGACTGTAGCTGTTGAAAGAATAAAGAATAAAGCAATTGCAACTAAAGCAATAATTGAGCCACCAATTAATGAAATTGAAGTTGAATTATGAAAAATGTTATTTATAAATTCTTTACAAGCAGCAAATCCATCCTTCATTGAAATAGCAACTGCGCACATATTTCAAGTGCCAAAGAATCCTACTCCAAATTCAGTTGTTGCTGGTTTGGCTTCTTCTTTGATGATCATTTCAGGTTGCATACCAAAAATGAACAATAAACAAAAGCCAACTACAAAAGCTAATAATACTATTAGCGGAATAAATTTAGTTCTACTATACATTATTATTTTCCTTTTTTTAATTACTATAATTATTTTATAGTATATATTTTAATTTTTTAAAAAATAAACAAGACTTTAAAACAAGACCACTATGGGTCTTGTCTAAATTTAGGCAGATTTTATTTCTTTCCTCTGTAACCAAAGTCAATAACAGCACGTCCTAAGAATTCACCTTTTTGAAGTTTTTCAAATACTTCTGAAACTTCTTCTAGTTTAATAACTTTTGTAACTTCTGATTTAACTTTTCCTTCTGCAGCATATTGAAGTGATTCTTGTAAATCAAGTCTTGTACCAACGATTGATCCAGCTAATTCTTTTTCGAACAATACTGTTCAGAATACTGAAACGCCAATGTTGTCTTTACCAAGTTTGTCTTTTGATGGAAGGCCTACTAATACTTGACGTCCTCCACGACGAAGCATTTCCATGCCTTGTTCACCAGCAATTGTAGCAACTGATGTGTTAATTACAGCGTGAGCTCCACCACCAGTCTTTTCAATAAGTTTTTGAATAAAGTTTGGATCTTTCTTAGAGTTGAATGCAAATTCTGCTCCTGATTTTAAAGCTAATTCGCATTTTTCATCTGTTAAGTCGATACCAATAGGTCTATATCCCATTGCTTTAGCATATTGAATTGCTAATTGGCCAAGTCCACCAACACCGATAACAACCATTCAGTAACCTGGACGAGCTTTAGCTCTTTTAATTGCCTTATAGGTTGTAACACCAGCACATACTACAGGTGCTCCAGTAATTAAATCTAATCCTTCTGGAACAATTCCTACATAATCTTCGTGTCCAATTGCATATTCAGCAAATGATCCATCAAATGTGTAAGCTGACATGTTTTGTTTAGGACAAAGTGTTTCTTCACCTTGTAAACATCATTCACATAATCCACAAGCATCATGTAATCATGCTAGACAAACTCTGTCACCTTTTTTAAGACGTGTACATCCTGGACCAAGTTTTTCAACAATACCGATACCTTCGTGTCCTGGAATTAATGGATATTTAGGTTCTACCAATCAGTCATAGTTTGCAGCGTGTAAGTCTGTGTGGCATACCCCTGAAGTTTCCATTCTAATAAGAACTTGTTTAGGCCCTGGTTTTGGAATTGGAACTTCCTTAACACTTCATTGTTTTGGTTTTTCAACAACAAAAGCTTTCATAGTTGTTTTTGCCATAAAATCTATCTCCTTAAAAAAATAATAGTAATTTTTTAATGTTTTATAAAACATTCATGGGAACATTCCCATAATTTAATTCTATATTTTTTTTAAAATAAAATTGCCATTTGAAAAAAATACTAAAAGTTTTCTACAATAGAGTGAACCCCAAATTCCGTACTTTTTGGAAAGGGGTTCATTTTTATGCAATTTAAATTTAAAAAAGTAAAAAGAAACAAATGAAATAGAGATATAAAAGGTTATTTAAAATTAAAACTTGATCAAAAGATAAAAATTATCGAATTATATTTTCAAGAATTTAGTATTTTAGAAATATCTAAAATAATGGAAAACTCTTATTCAGCATGCTATTCAGTAATAGAAAAATACAAAAAGTTTGGTTATAATTCTTTTGCTATGGAAAAGAAAAAAGGAAGAAAATCTAAAATAAATTTAGATGCTCAAAAGGCAACAAATTTTAAAATCAATATTGAAAATAAAATAGAAAATAAAGATTTATTAATTAAACAATTAAAGGAAGAAAATAAAATACTCAAATTGGAGAATGCGATAGCAAAAAAAGTGAGCGCCTTGGTTCAATTGAAAGACTCACTAACAAAGAAAAATTCCAAATAACAATTGAACTAAGGCAAGAATTTAAAAAGCTATTTTTTATTAAATTAATATTAGAAAAAATTAAATTGAAAAAGTAGAGTGAACCCCAAATTCCGGACTTTTTGGAAAGGGGTTCATTTTTATGCAATTTAAATTTAAAAAAGTAAAAAGAAACAAATGAAATAGAGATATAAAAGGTTATTTAAAATTAAAACTTGATCAAAAGATAAAAATTATCGAATTATATTTTCAAGAATTTAGTATTTTAGAAATATCTAAAATAATGGAAAACTCTTATTCAGCATGCTATTCAGTAATAGAAAAATACAAAAAGTTTGGTTATAATTCTTTTGCTATGGAAAAGAAAAAAGGAAGAAAATCTAAAATAAATTTAGATGCTCAAAAGGCAACAAATTTTAAAATCAATATTGAAAATAAAATAGAAAATAAAGATTTATTAATTAAACAATTAAAGGAAGAAAATAAAATACTCAAATTGGAGAATGCGATAGCAAAAAAAGTGAGCGCCTTGGTTCAATTGAAAGACTCACTAACAAAGAAAAATCCAAAATAACAATTGAACTAAGGCAAGAATTTAAAAAGCTATTTTTTAATTAAATTATATTAGAAAAAATTTAAATTGAAAAAGTCAAACTTTTTATGAGAATTTTAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAACTAAAAGGCAATTTTATTTGTGTAAATTTCATTTTTTAAGAAAATTTAATGATTTTATTTTCAAAAAGAGAAATCGTGGATTTAACAATTTTATAGATAGCATTCAAAATTTAAGTGAGTGAAAAATCGAAAAAGAAATATCAGAAGCTTACAACAGTAGAGATTATGGAAAGTTGTTAGATATATTAAATACAATTTATCTCTATTTTAGAGACTCTTTTGTTTTGAGCGATATTGATAGATACAATTTAAATAGTTTGATAAGATACACATCTAAACTTGAAAGTGGAATCATTAATAATGATTATAGTTCACTAATAGAAGGACACATATCTGTTTATATTAAAAGAAAACTAAACAAAAGCTTTGCATTATATTCTCAAAAAACAATAGAAGCTTTATTGTTGAATGATAATAAAATTCAAAATACTCTTATTCAGACATATCATTAGTATTTATATTAATATTAATATAGAAAGGCAATTAAATTTGCTATTTTTTCCACGCAATTTTCTTTGTAATTTACATTTTTTGATATAAAATATAGTTGATAGAAAAAGGAAATTAGAGTGAACCCCAAATTCCGGACTTTTTGGAAAGGGGTTCATTTTTATGCAATTTAAATTTAAAAAAGTAAAAAGAAACAAATGAAATAGAGATATAAAAGGTTATTTAAAATTAAAACTTGATCAAAAGATAAAAATTATCGAATTATATTTTCAAGAATTTAGTATTTTAGAAATATCTAAAATAATGGAAAACTCTTATTCAGCATGCTATTCAGTAATAGAAAAATACAAAAAGTTTGGTTATAATTCTTTTGCTATGGAAAAGAAAAAAGGAAGAAAATCTAAAATAAATTTAGATGCTCAAAAGGCAACAAATTTTAAAATCAATATTGAAAATAAAATAGAAAATAAAGATTTATTGGAAGTGATAGAAAGAAGTGTGTTGAACACTTTTTTTATTTCACTTTTGATTATTTATAAAAGTACTCATAAACATTAATTCATATTTTTATTTAGTTTCGTATTCTGCTAAATTAATATACCTTTCAAAAAAATGATCAAAAGTATAAAAAAATAACAGCGCCTAGAACACCACCAATCGTTCGACCTGTTGTATATATTATACCACAGTCGCTGTTATATGTTCAAAAAAAAGGAGAAATTATGAGTGTATACGGCGAACTAAGGTTAATTGCAAATGAAGGAAGCGAAAACGAAGTTAGAAAGTTTGAAGCTAACTTGGAATGGAGAAAAAATTATTTTGGCAAGAAGGTTTATGACAAGTTAAGCCAAGATACAGTTAGTGAAATTTTAAAAACAAAGATAGTACTCGGTGAAAGCTACTATAAGATTTTGAGAACTGAAAAAGTTGCTTTTGAAGTGTATGTCTGTTTAAGGTTTCTTGGGGCTAAAAAACGCTATGTAAATTTTTATGAATTAGTGCTTTGGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAAGAAAACAAAACAACTCAAAGCATGTCAAGAAAGGGAAATGNTTTAGACAATAGTCCTACTGAATGTTTATTTAGTGTTATAAAAAGAGAATTTTGATTTGAGAAAGAAAAGAAATTTAATAGTTTTAAAGAATTTAAAACTGCTTAGGGAGAATATATTTCATATTATAATAATGACAGAATGTTTAATAAATTAAAAGGACTTAGTCCTGTCCAATACAGGAATAAGTCCAAACATAATTAAAAAGTCCAATTTTTGGGGTTCATACCAATTTATTCTCTTATTTTTTTAAAATAATTTATTCGCTTTTGTTAAATCTTCATTTATAATGAATACCACAATTAAACATTGGAAGAAAAATTTTATTATGAAAAAAATAAGTTTTTAAGGCATTTAAGTGCTTTTTTACCTTTAAGGTTATTGCAATGAGCACATCTTGTGAAGAATAAGAAAAGAAGCCTGAATCAGTTAAACCTATCAAAAAGTTAGCTAAAAAATTAGTTGAAGATTTAAATAAATTGAATAAAAAAATTAGATCCGGCTAAATATGAAGTTAAATTAGAAATAAATTCAATTATAAAAAATAATAGTGAAGTAACATTTAATTTTTACTTACAATTGAAAAGCAATGCTAAAGTTAAATCAAAAATTAAACAAGTAAAAATTAATAATTTCAAAATAGTTAAATTAACAAAATCTTTTGAAGAATTATTTTTTGGAGTTAATGATTCTTCAAAATTATCGGCCAATAACGTTATAACTTTAGATCAATATGTTGATAATTTAAACTTATTTCAGAGCAAAATTAATCTTAATTTAAATTTAATTAATAAAGAACTAAAAATTTAAAAATAAGAGTTTATTTAAATGACAATAAATATAAAAAGTTAAAAACCTAATGATTTTAGCTTTAAGTAAAGAATTAAATTTAAAAGATATAGAAAATTTATTTTCTAAAAAGAATTTTTACAAGCTTATTTCAAATATGTAAAACTTGTTTCTGTAGACTCGAGAGAAAATATTTTTAAAGATAAAAATTATTTTGCTTCAAGATTTAGACAAGGTTTTGAAGATACAAATGAAGCTTATACAAGTAGATATTTATATACAAGCAAATTGCCTAAATATGACTTATTCAAAAATAATGACAAAAAGGTTATTTTTAAAACTAAATATAGTTATGATGATAAAAAAGTAGTGCATTTTTAAGTTATTATTTATATTTTGATGATGGTATTTATGAAAAGGTAAGAACTGACGAAAACCACTTTGAAATAGATGGATTCATTCAAATTAATGCTACTAAAATGATATGTTTTCAATAGTTGCTAATGATAAATTTTTGGGCCTAGCAGAAAGTGCCAAAAAATTTAAATATGTATTACAAGATTTAAAAAATAAAGGCGCTACTAACTACGAATGTGTTGAACGAAATATTTTAAAAACCGTATTTAATAGTAAAAAAACACAAATATTTTTATATCAAGAAAATAATAATTTGAGAGCAGAAAATATTGTTAATTTCAATCTTAAATATTTTAGCAATAGTGTTAAAAATGAATTAAATGATTCTTTACTTTCTAATGATGATTTAATAAAATTAAGCTTGAAAAAGCTTATGTTGAAATTACCAAAATATCTTTAAAAGGAAGAAACTAACAAATATATTTTTAACATTCATTTCAATTTAACTATAACAATAAAAAAGGTTGAAACTATAATTTAGCATCCTTCGATTTAACTTTTGAAAATATAATTTAGCCTATAAGTTTTCAAAAGTTATAATTTATTAAACTAGTTAATATATTTTTTTATTTGTTTTATTGATTCAAAATATTAATTTTTATATATTTTAATATATAATTTTTTAGTAAAAAAATATAGAAAAGTATTGTTTTAAGGAGGAAATATGTCTACAAAAACTATTTCAAAAGCAACAAAAGGATTAGGATGAGCTATTGCAACAGCTACTATAACTGGCTCAATTCTATTAACTATTATTGCAATAAAAACACAAAATACTAAAAAAATGTCTGATGATGATTTTCTAAAAAATTTAGAAATTAAAGGACAAAATGTAAGTGAAATTTATGCTAGTGATGCAAAAATTACAAATGTTGAATACAAAAAAGATTCTAATTATGAAATTAATGTTTCTCTAGAAGGATCAAATGTTCTAAAATCAACTGCATACTACAAAATTGAAATTACAAATAAAAATACAAAAGTTAAAAAGTCAAAAATTGTTCCAATAAGTGGTTTCAAGAAGTTTGATGAAAAAAGTACTATTTTAAAAGAAAAAGAGCTATTTGAAGATAAATCTAAATTAAAAGAATATTTAGATAATATGCCTAAAAATGATTCTGAAAATCTTAAAAAATATATTTCTAAAAATAAACATTTTATTTACAACGAAATACCAAGTTCATTAAAAGCTTCATTTAAAGGTTATTCTTTCATTCAATCTAATGGTCAAGATGCTGTTAGAGTTGTTTTCGTTTTAAATGGTCATATTACAAATGATAATCTCGAAAATAATATAGTTAAAAAAGCAAATAAAGAATATAAAAATAATGAAAAAACTTTTGAATTTTACTTAAGTAATTTAGTAAAACAAGGAACATCAAGTTCAGAAATTCAAGCAAGAAATAACTTAAGAGATTTAAAAGAAAAATACGAAACTCTTAAAAAAGAAGCTGAAGTAGCTAAATCGAAAGTTACAGGTGCTATTGTTGCTGAATATGAAAATGTTTTAGTTTCAATTACTAAAAATATTAATGAAATCGACAAAATTTTAAATGATGCAACAATTAAAGCAGCAGATCATGTTGATACATTAAAAGTTAATTATGAAAAAGACTTATTAGATAAAAATACTGTAGATTTAAAAGCAACAATTAATGATGCTGAAGTTACAAGAGATAACTATAAAGCTAAAGATGAAACAAATTATAACAATCCAATAATTGAATTTAACAAAGCTATTGATCAAGCAAAAATTAAACTTGCTTCAACAAATATTAAAGAAATTAATGATGAAATTTCTAACTTAAAAACTAAAAAAGAAGAGTTTATTAATGCTTTAAAAACTATCTTAACTGACACACAAAGTGATTCAAAAACTAAATTGTTAGAATCAAGCGATTACAAATATTTGATCGATGATATTAAAGCTCGTAAAGCTACTTTTGATGCTACACTTAATGAAGATAATATTCTAAGTGAAATTGAAAACTTAGAAGTTTTAGTTCAAAATACTCAAACAATTGATGCTATTACACCAAAAGCAATAATTGAAGCTAATAATAAACTTCTAGCTTCCTTACTAGCCTATAAAACAGCCTTAGAAGCAAAATTACACAAAATAAAACAAGATGTAATAGACTCAACTTATGACAAAGATTCATTGTCACAATTTATCGAATATTTAATTAAAAAAATTAAAACAACAAATGAAAGCAAAGCTTTAGTTAATAAACAAATAGATTTATTTAAAGCTTATTTAACTACACCAGGTTTTGTTCAAAAATATAATTCTTTAGAAAAAGATTTTGATAAATATGAAGCAATTTTTAATGAAAATATAAATAAAGCTAATGAACTTATCGCAACTCTAGATGCAGGATTAGATCAATATTCAAATTCAAAAGATAAATATAGTGAAATCAAAACTAAAGTTGACTCACTTTTAGCAGAACAAGCAACTCGTTTAAGCTCAATTGGAACTCAAATTGTTAATAGTGCACTTATTGATTTTGATAATTATGTTCAAAAGCAAAAAGCTTTAATCAATACTTCACGTAATAACTACAAAACAATTGTGACAAATGTTTACAATTTATTCAAAGATTCAGAAATCTACAAACAATATATAGATGATAATAAAGCGAAACTTACAAACTCAACTATTGATGTTAAAACTGCTTTTGAATTATTAAAAACTTTATATACAAAAGATAATGAAATGAATCAAAAAGTTGCAGCTCACTCAAACTTAGCAACTAATAGCTACTGAAATTCATTATTAGATGGCGATTTAAAAAATAACTTTACAATTCCAATGCAAGTTAATGCTGATTATGAAAATTACTTTAAATTAGATGACCATTACAAAATGGCTTTAGATATTATTATGGACAATTTAATATCTCAAATTCGTTCAGAACATATTAAAGGTATTGAAGCGAAACAAGAAGAAATTTATCGTATTTTCTATGAAAAAATGCGTGCTATTTATAATGCACAATTAACTAATGGAGTTAAATTTGTAAATGATAATATTCTAGATGCTAAAAATAGAAGAATTCAAGCATTAAAAGAATATGAAAAGGCTTTAAAAGAAGCAGAAAATTTAATCAAACATTATACAGATGAACACTATAACAATACTGATGATTTCTTCAAAACACTTGAAGAAGGCATTAAAGCTGTTAAAACTTTAAATAAAGATCGAGCAATGCTAAGTCTTGTAAAAAATACAACTATAGAATTAAAAGAAGTTATCAAAAATTCACAAGATCAATATAGAACATTCCTATTGTCTAAACTTGATGAAGCTAGAAAAAATTGACAAATTTATTCAAACAGCATCAATGGTCCAGAACATCAAGATGTCTTTCCAAAAACAATTAAACTAATTAATGAAAGTTGGAAAAAATTAAAAATGCTATTGACAATAGTACAACTATTACTATTAAAGAATTAGAAGATTTAGTAAAAGAAAACAATGATGTATATGGAAAATATACAAGCACTAAAATTCCAGAATGAAACGCATTTATAAAGAAATTCGATAGTGCTTTTGAAGAATTAGAAGCAATCAAAAAAGACTATAAAAAAGAAACAATCGTCAAATTAGGTAAAGACAAATATCAAGACAATGACTTAGTTTTAAATCCTGCTTTTGAAGCTAATTCAGTTTATCAAAAAGCAAAAGAATACAAAGAATCTGGAAAATTAAGTGTAGAAAAAAATCTTAAAACATCTAAAGAACATATAACTAAAATTAAAGCTAAAATAGAAGAAATTAAACAATTAGTTAATTATTATGAAACAAAATGGAATCAAGCTAAAACTAATTTAGATAATACAATTAAAACAGTAGAAGAATATATAACTAACAATCCTTCAGCAACAAATATCGATCAACTTCAAGCACTTCTTAATTCAACAAAAGAAGCTATTGAAACTTCAAAATTAGAAGAAGTTAATTTAACAAAACTTCAACAATTAGATAAAGATCTTGAAGCAAAATATCAAGAAGTATTGAATAAAAACAATAGTTTAGCAGCTGCTAAAAATAAATTACAATCATCAATTGAAAAGGTTGAAAATCATTTAAATTCAAGAAATTCTTTAGACAATTTAGATAATTCATATTACTCAAATTTAATGCAAAATGAGAAAATTGATTACAATGAAAATAGTTCAACTGCTGCTTCCAAAAAAGATTTAAAATCTATATTAGATTCAATGAAAAATATTGTTAAAGGCAATGATGAAAATGCAATTAATCACAATGTTCAATTTGGTCAAAAATTAATTGATGCTATTGAAGAAAAAAGAACAGAAGACATAGTTGCAAAACAAAATACATTAAAAGATAAATTAAATGAATATAACGATTTAAAAGATTCATTAAAATCATTATCTTCAAAAGATTATTATGGAGCTCAAATTACAAATCTTGAAGCTAATTCAACTGCTGATGAAGATGCTGATAGAATTACAAAATTAGCACGAGCTAACTTAATTAAAGAAAGCCAAAAATCAATTGTTAATACTAAAGTTGAATTAGAAAAATTAATTGGTAATTTAACTAAAATTAAAACTGATCGTGAAGCTCTCATTCGACAAGCAAATGATCAATATAATACAAATAAAGACAAATTTGCTGAGCTTCAAAAACAACTAAATGATTTAAATATTACACCTGCTACAATAGCGGATTATAACACAAAAGTGAAACCTAAACTTGAAGCAGCAAATAATAAAAATGAATTATTAAAAGAAGTTTCTAAAGATCAAAATAATAGTAATATAACAGCTAAAATTAATGATTCTAAAGTTGCAATTCAAGATTTAGAAAATGTTGTAAACAACTATAAAACAGCTCTTAACAACAAAATTAAAGAAAATATTTCAGCAATAAAAGAATTAGTACAATACATTAGAAACAATCCAAACAATTTAACTCTTCCAGCTAATTTTGTAACCAATTTAAAAGCTGTAGAAGACAATCAAGACTCAACTGATATTGCTATTTTAAATCAAACAAAAACTCAAAGTGAAGCATTAATTAAAGAGTTAACCGATTTAATTAATACAGCAGTAGCAACTGTTAATAATAAAGCTCAAGATACAAATCAAAAAGTTGAAGAAGAAAAAGCAAAAAATGAAACATGAATGACTGCTGCTCAAGTTCGAGAAATTCAACAACTTTTTGATAATTCTCAAGCCTTATTTAATGAAGTTAAAAACGCTGCTAATAATAAAGATCAAATCAAAGATGTAGGTGTTGATAACTTAACAAATAATATAACTAAAGCAGAAGCAAAATTAAAAGAAATTCAAAACAAAAAAGCAGAATTTAAACAAGAAAATACTAATTTAAGAAATCAAATTAGTGAATTAATGAATGATTTAAAAGTACAAGCTGATACACTTCCAAATGAATTTAATGAACACATTTTAGCAACTCATAGCTCAATAAAAACCATTCAAGAATATTATGAAAATACATATAGCAAAATTAAAAATCAAGAATGAGAAAACAAAAACATTAATGATTTACAAACTATTAAAGATATTCTAGATGGTTACAAACAATCATTAGATAATTACAAAAATGAAGCTGCAGCTGCTTATCGTAATAAATTAAAAGAACAAATTCAAAAATCAAATAATAAAGCTCAAGAATTGACTACCGCTAATGATGAATTAACTGGTATTAATAAAGTTGATAACTATTCAGATTCGATTACTAAATTAAATAATCAAAAGAAAGCAGAATATCAAGAATTAATTGATCAAAATAACAATGATCTTAAAACTCTTAAAAATGCTTTAATTGAAATTAAAAATAGTAATGACTTAATTGAAATATTAATTAATGAAGTTGTCAATAAAAAATGTAACTTAATTAATGCAACTATTAAAGAATATGAAGATCAACTAGAAGCAATTAATAATTCTATTTACAGCGATTTATATGAAGACATTACAACAAGTTTACATTTAGACTTTTTAAACTCTTTAGCTATTTTAAAAAATAAATATCCAAATAATTTAAATAAAGTAATCAAACTTTCAGAAGTAAAAAGAGATTATGAAGAAATTGAAGGTAAACTTAATGATTTAAAAAATAGATTAGCCGCTACACAAAATGAAAGAAATGACATTCTTGCAGAATTAAGATTAAACTTTGACAAAATTAATTCATATGCTAATTATTCAGATGCTGATAGTAAATTGCCTTTAACTAAAGCTTTAATAAATGATGCAAATAATAATTTAGAAAATCACTTTAGTTCAATGACACTAAAATACTTAAAAGCTGAAAAATCTGCTAGTGGTCAATATACAAAAGTAATAAATGAAATAAATCAATATGAAGCAGATCTTAATGTAGCTTTACAGGAAATTATTAATGAAGTAAAAGATGCTAAATATCAAAAAATAGATGACATTATTTCAAATAATTCAAGTGTCTTTTTAGCTGACAAAGATGATTATAAAAAAACAATTGCACCAGTTAAACAAAAAGCTAATAATGGTGTTAATGACTTAAAAACTTTAATTACAACAATTAAAGATGCAAATGAAAAACTTGTAGCTATTAAAGCTCTTGGCAATAGAATTCTTACAAAAATTAATACTGAATTCAACAATAGAATTAACCGACTTAAAACTATTGCAAATGAAACTGATGGATTCTTAAGTCCTAAAGTTGCTGACGGTAAATATGAAGGTTCTATTTTGCATGGAATTAAACAATCAAATGATGAAATTATTAAAATAATTAAAACAAAAATCGATACAATAATGAACAATAATAGTAACCTTGCATACTTAGTAAAAATCGATAATTCTAGTGATAAAACTATAGAAAAATTAGATCAATTAATTGCAAGAGCAACCCAATTTAAAGATAACGCAATCACAAAAACTAATGAATATGAAAGAGCATGAGAATAAGCTAAAAATGAAAATCTTCAAAAACTAGATGAAGCTAATGGAATTATTCAATTCTTAAATGATAAAAAAACAACAAGCACAATAACTAGTGAAGAGCAAACGGCTTTAACTGCACTAAATACTGCTAAACAAAATTATGATCGAATTATTGAAAACAATATTGATCACAAATCAAAAACTAAAATTGAAGATGCTACTAATCATTTAAATGATGCATTAAATACTGCTCGTCAAGAATTGAAAAATTCACAAAAAACTACTGCAATTATTAATTTAAAAAACCTTAAATCAGAAATTAATGCAGACTTAGCAAAACTAAAAGGCTATTCATCATTATCAAATGATCTTACTAAATCAATTGGTGATAGCTTAGTTCAATATAAAGCTTCATTACAAAAAGTAATTGATGAAATTGATGATTTAGTTCAAAAAATCAATACCATAGGTTTTGTTCAAAGTGAATATGATACTGTTTATAACAAATATAAAGATGTACCAAATAAAGACGAAAACATCATTAAATTTATTGAAAATAAAGTATTTGTTGAAACTAAATTCTTAAACAAATATGACATCAAATTAGATGAAATAACTAATCAAGAATTAAAAGATAAAGTTACAACTAAATTTAATGAATTCAAGGCAGATATAGTTAACTTCTGTTCACAAAATATTACTAATTATGATGCTGATGTTGCAACACTTACAACAAAACATCAAGCAATTCAAAATAAATGAAATGCCGACAGTGAAATGATAAAAATTGGTAAATACCTTAAATATGTTGTTGAAGCAACAAACTTTGAAAAAGCTATTCAAGCAATTGAAAATTCATATAACAATGAACCTAGCGGATACAAAGATGTTGAAGCTTACAATAACTTCAAAAAAGAAGAACAAAACTTAGCTTCTGGAAAAACATCTATTGAAACAATACTTAACAATTTCAAAAACAAAAAACAAAGTGAATTAATTAGTGGAAATGTTCCTGAAGATGCTCAATGATATGAAACTGAAACAGCTAGAATTAAAGAATTAATGTCAAATAATAATCAAATAATAACAAATAAATTGCGTGAATTTGATGAACAAAATATTGAAAAATTAAGAAACTATGTTGAAGAACTTAGACAATTAGTAAATATTTATGAACATGCTTCAATAAAATTTGCAAGCACAAATTTAAATGACATTAATAAAGAAATTAGTGAAGTTGATGCTTTAATTGCAAATAGCAAAAAAGCCTATAAAACAGAAGATAAAGTAAAAAATAGCGCTGCAAACTTAAGTCAAAAAACACTTAATACTTACAATGAATTTAGAACTCAAACACTGAATAAATATAAACAATTAATGAGTCTAAATGTTACTCCTGTTGTAGCAAATGTTAGACCTTGATTATTATCTAGTGATCCTCAAAATGATATCATTGTAAACCAAAGAGAAATTGATGTATTAAACAATTTAAAAACAAATAAATATTCTTTAGATAAATTTAATGCTAATTATCCTGCTAATTTAGCAACTGTTAATAAACACAAAATAGGTGATTTATTAAGTCATGTTGAACAATTAATAAATGATTATTCTATTATTAAAGATGATATTGAAAATGGTGATTTTGAAGAACTTAAAAAATTAACTGAATTATATTATGAAGCAAGATATAAAGCTAAAGATGTTGAAACTAAACATAATAAAGAATACAAATTATTTGTTGAAAACTCATTTAATGGGCCAAATAAAGCTTTAGTTGAAATTCAAAATTCAATTGACATGCATAATCGTCAATATAAAGAATTAAACGAAAAATACTTCTTTGAAAGATTAAATTCATCAAATAATGCTAAAATGACTAGTTTATTAGTAGATAACTACCACAAATACTTTGTTGATTTAAATGGTAAATTACTAGGTCAAGTATCATTCAATAATGAAGATGAAAAAATTAATAAATATAGATGATATGCAAGTATTGAAACTCTATACAAACATGCTGAAACCTTTGGCAATGTAGCCCTAGCTCGATACATTAAATATTCAAATGTAATGGATAGTTTATATAGAGATTTAGGTAAAGATCCTGGAAATCGTAGAATGAGACATAACAATAACAAAATTGATTGAGAACGAAATATCTTAATGGGTTATGACATGGCTTTAGATGAAGAAATGCTTGCTATTAATGATTCAAATCAAAAATTTGATGATGGTTATAGAACAGTTATGTTAGTTGGTGGATTTGCTCAATATGTTAAAGATCATCTTAATGACAAAAATTCAATTTATGCAGCTAATAGTGGTTTACAATTATGATCATATATTTTTGATGAAAAAACTCAAACGCTTGACTTTACTGCTTTAAATTATGTAAATGAATTTAAATCAAAAAATCAAAACATTAAAGTTGCTAAATATGTTTTTGACTGAAATAGAGGCGAAAATGTTAAAAATAAAGAAGCTGTTAATAAATATTTTACAGATTCAACAGTTGCAAAAGAATTAAGCTATAGTGGTGTTGCTAAATATGGTGAATTATTTAACTTCAACAAAGTTATTATTCCAGATGGCAATTTATTTTCTCAACAAGTTGTAATTCCTTTATTAAGAGATGAAACAATATTTAAAGATGCTAAAGGTCAAAAAACAACAATTATTTTACCTAAAAACAACTTTAGATATAGTTATAGATTACAAACTTTATATAGTTTCTATGAAAATGAAAGTACAGTAGGAATTCCTTGTCCAGGTTATGCATATGATAAACGTTATAACACTCAATTTATTGAATTTGTAGCTGAAAATATGCAATTCATGGCTGATACTTTATACTCAGAAAATATGTATATGTGAAATGGAAATTGACAAAATAAAGGTATAAATCATCAATATTACTTCCCAATTTTCAAATATCATTTCTCAAAAACAAACAAAAGTACTTCATATGTAGATATCATGAATACATTCAAACATGTATTAGATTCTAGATATTCTCTTTTAGCAAATACTCCATATACTTATTGTTTAAATATTGAAAGTTTTGTTGCTTATAAAAATCACATAATTGATGATAATGCAAAAAAACAATATAACCAAATAGTTTCTAATATTACAAATAGAATGAATTCAGCTTCAAGCAAAGAACAAGAAGCTAAAAAAATATTAGAAGAAATAAACAATAAATTACAAACGTTAAAAAATAATCCTAATACGCACTATTTTGGAAATAAAAATGTAGTAGGTCGTTCAATTGATCAAAAGCCACATATTACATTTAATATTGCTATTTATGATGAAAATCAAAAACATGATTACTACATAACAAGTACAACTAATTATCGTTTCTTCTTAAAAGATGTTAGCAAAGATATTTATTAGAAATTTACTTGCACAATTTGTGCAAGTTTTTATTTATTTAAAATTAAGCTAAAATGGTATGTGAACCGGGACTGTAAAAAATTTTGTGTGATGTCCCATGAAGAATAATTTTTCTTCAAATTTTAAAAGCTTTGCTTCTTTAGTTTCAAAGAAGGTCTCTCCTTTAATTGAATAAAGGGGAGAGTGGAGAGGAATTAAGATTTCTTGCATAGCAAGATCTTTTTCCTATTTTAAAATTTTAATTTTAAAAATAAGATAATGTTTTTTAAGCATTATCTTATTTTGTTCCACAATACTATTAGGTGTTGACGCAAAGTCAATTCCTTTCTATTGAAAAATGGAGAGATTTTACACAAAATTCTAAGCAATTTCAATAATACTTTTTGTATGGAATAGTCATAATATTTTTTTAATTTAAATATTAAATGAACCCCCTTTTTTTTGAAAAAGGAATTTTGGGTTCAATATAATATTAAATATCATTAATTTATCAAATAAGTTTGCTGTATGTATTATTACTTGTTGCAGTAAAGATATTTGATCATTTATCTCAAATTGAAACTGTTTCAGAAGGCGATGATTTTTTCAAAATGTATTTCTCAGTGATTGTATATTCTGATTTTCAGTACATTTTATTTCATATGTAGTATGAAGCATCAAAGTTTCCTAGTGGGTTATCAAGAGTAAATTCAAAGATGTAATTTACTCATGAAATAGGAGCCCTGTTATGTTTTAATAAGAAATGAGCTGAAGAATGGTCTACTGTTCTTAAATATGAGAATAATTTATTAGAAAATGGCATATATCCAAATAGTGGGTTAGTACTATCATATTTTTCATCTGATTTTCCAAGAAATGATCTATAGTGAGGACCGTTGAATCCATTTATTATTTCTTCATCTGGATTTTGTTTACCTGGAGTTTTAAAAGTTTTATATGGTTTTTCTTTGCTATAAACTCAAACTCTATTTCTTAATGGATTATTTCCAATTGCGTAATGAGATTTGTTTTCTTCTCCGCTTCAACTTTCTCATCTTTCTTCATAATATCCATTGCTTGTTGGATTGTTGTATTTAATGAAATCAAAAGCATATTTTATTTTGTAAAGAGCATCATTTAAATTAACAAAATTGTCTTTAAAATCATTTTTAAGCATTATTTTAGAATCTGATATTTTTTTGATATTTAAAAGCATTTTTTCAGTTATTGTTTTCTCATATTCTATTAATTTGGCCAAAGTATATAGATCATCATGTCCACTTCAAATATCATCATGTATTGGTCCTGCATAATCAATATAAGATTTATTAGGATATTTTTGACTTATATATTTAACTGTTGGTTTACCCGGTTTACCAAGTTCTTGAACTTCAAAAATTGTATGTCAAGGGGCATAATGATCATTTTCAATTTGTTTACCTTGTTCGATTTCACTAGTAGTATTTCCTTTATCTACTCCATATCGGTGTCATGAATATTCGGCTCTAACTTTATCACCTTTAGAATTTCCTCAGCCTCCATTTGTGTTACCTTTTGAGTATCCTCTAGTATCTAAATCTGAAATATGGTCATATAGATGTTTTTTACCTAGAACTTTAGGTTTAGTTCAAATTTTTGATTTTTCATCTCAATTTACATTATTTTGTGAGTCAAATGTTGGTCTATACAATTTTGTTGGAGATCAGTATACATCACCATAATTGAATTTTTCATGTAATAAGTAAATTAGTTTGTTTAAGAAATATTCTCTTAAATCTTGAACAGCTAATAAATAATCTGTTTCATTTAAATAATTTTTAATTAAATCAATGTTTGTAAAATCAACACCACCATCAAGTTGTTGCAAAGCTTTTAAACCTTGACTATCTAATTTAGAAATTTGATTATTTTTAATTGCAATCATTTCATTTTTTGCAAAAACAAGTGAACTTTCTTGTTCTGAAGTTAATTTTGCTTTTTGTTCAGTTTTCGCTTTTTCTTTTACATATTCATTATAGTAATTAAGAACACCAAATCATTTTTTAACAAAGTTAACTTTATTGTCTTTATCAGCTTGAACAATACTAAAGTTTTCTGTAGAATTAACATTTTTATAAGCTTCAATTGCAGCATCAAAAATTTTGTTTTTATATTCATATACTTTAGAATCCACATTTGCTATTGCTGCTAATTTTGCATCCATTTCGTTGTATGATTCTAAGCAAAATTGAGCTTCATTTTCTAAGAAATTACTATAAAGTTTATCTTTTTCATTAATTGATGAAATAAAATTATTGATTAAATTAATTTTTTCAAATAGTCCTTTAAATTTTTCTTTGTCATTTTGAATTTCACTATTTTCAATTGAAGATATTTCTTTAGTTGATAAATTAGGTTTGTTATTATTAATATCTTCAATTATTTTTTTAACATCTTCGAATTGGTCTAATGTTTTTAAATTATCAGAAATTTTTGATAAGAAATCAGCAATTTTTGGATTCGCATTACTAATAAGTTTTGAAATTTCTTTAGGTTTCGAAGCAAAGTTATTGACTTTTTGTAAGTTTAAATCAGTTGCGTTTTTAATAATTTCAAATATTTTTAAATAATTAGTTTCATATTCTTTAAGATTTGAAATAATTTCAAAGAATGTTGAAATATTATTAATTTTGTTGTCAATATCAGAATCTAAATTAGCTTTTAATAAAGGATCTAGTTTAGTTTGTATATCAATTATTGATTTATAAGAAGTTTCAAATAAATTAAATCCATTTAATGTTGTTTTGTTTGCTTCTAATGCTAAATTAAATTCATTTAATCTTGATTTAATATTAACAAAGGCTTTTTTAAATCCATTTTTTATTGAATCTAATTCATTTTTGCTATTAACATATTCATTAATTAATTTGTTTCTGCCTTCAATATACTTATCTCATTTGAGAGAACTTAATTCAATTTTAGATTTTTGTACAAATTGATCAATAATTTGTTTTGAGTTATTTAGTTTAGCTAATTTAACTGTTGTTTCAATTGAATCGACATTTGAATTATAAGCATTTAAAAATTCTTTAATAACAGACTGAATAGAAGTTGTCAAATCATTTTCAACATTTAGAATAGCTTGAATTTTGTTAATAAATTCATCTAATTTTTCAAAAGAAATTTGATTTGAACTTTCAACAAATGAGTTAAATTCATTAAGACCATTAATAATAGGTGTTAATGATTTGTTGCTTAAGTCTAGAATTTGAGATTTATTAAATAATTTTGTATTTTCAATTGTTTTAACAAAAGTATTAACATAACGATTTATATATTCCTTCGTATTTCCATATGATTGTTCTAATGTTTTACATGCCTTAATAATATCTTCTATAATAACGCTTCTATTTACTAAAACACCGCCATTTTCTAAAATAACGTCATAATTATTAGAGTCAAATCTTGATAATAAAATCTTTTTAACAGCATTAATAGGATTTAAAATTGGAGAATTATTACTTGCAATAGTATTAGCATCTTCTATTTTTGCTGATAATTCTTCTATTTTATCTATGTTTGACATGAATTTTTTAATTAAACCATCTTTTTTAACATATTTTTTAATAATTTTGTCATTTCAAACATGAAAATCGGATTTAATAAAATCTGCTGCCTTATCAATCATTTCAGTTGAAATGTTAGGTTTTATGTTGCTTGAAAGTGCTTCTTCTTCAATTTTACTTAAAGTGTTTTGATATTCCTTAATTTTAAGTTCTAAAGAATTTAATTCACTTTTACTATTAGCATCAACAAATTTTTCATCTGCAGTTTTTAAAGTTAATAAATCATTACTACGAACAACAAAATCTTGGATTGATTTTTTGATATTTTCATATTCGCTTTGATTAATAACTTGTTTTGTTCTTTCTATCATTTCTGGTAAAAGATTTTGAATTATTTTCTTTTCATTACTGTGTTTAACAGCTCTTATATAACTTTTGTTACCAAAGTGGTGACCAATATGATATGAAGAGTGTTGATCAATAATTCCTTGTAAATCTTTAATAAGTTTTTCATTTGAGTCAATTTGAGTTTGGCACAAACTTAGTTCATTTCAAAGACTTGAAAGTCATGCTTGATTTTCATATTTCTTTCAAATTTCTTTTAATTTGTTGTCTTTTTCTTTTAATTTTTGATTTGATTCTTTAAGGTATTTTATATTGTTTTCAATTCACAATTTTTCTTTTTTATAGTTTGAATGGTCAACTGCATAACCAATTAAAGTACCAGCTGATACTGCACCAACAATTCCGGCAGAAATACCCATTGTTAAAGCTACAATTTTTTTTGTGCTATTTTTCATAGTTGTCTCCTTATATTTAACATAAAAAAATTAAATTTTCCGGACATAAGGAAAATTATGTCTTTATATTAAAATATTTATTAGACATTTTTGGAGGTCAAAAAACATGAAAAAGAATAATAATTTTCTTGATATTTTTTTGAATAATATTTACTCAATGGTTCAAAAAAACCAAAGAAGTTATCAAACTTTTAAACAATATAAAAGGTATTTATCATCCTTAAAAAATTGAAACAATGCGAAGCAATTAATTGTAAGGATGAATCGTGAATTAGCAAATAATAAAATTTCAAATAATACTAAATTAACTAAAAGAAACATATTTTCTTCTTTTTTAAATTGATTTTGCAAAATCACTAATACAAATATGGATGTTAAAAATGAACTTCTATATATAAAAGAAGAAAAAGGCAAAAGACAAGCTATACCTGAAAAGGAATTAATTAAACTTGTTGAAGAATTAAAAAGCTACAAAAACACCAAATTTAGTATCATTTTTCGTATTTTTCTTTCAACTGGTATTAGAGTTGGAGAATATAAAGAATTTATTAAAAACATTGATAAATTAGAAAAAGAGAATTGAAGTTTTATTATTAAAACATTAAAAAACAACAATCCTAGATACTTTAGGATTCCATCAAAATTAGAGATGCCTTTAATAGGTAATCTTAGAGAAGATATATTAGAAAATATAGATAAATTACGTATTTCTGAAAAATCAATTAAAAATATGTTTTATCTTTTTAAAAAACATATTAGTGAAAAATATCCTGATTTTAAATATAAAATCTCGGCTCACGTCTTAAGACATAATTATGCAACTAAAGCTTTTTATAATTTAAAAAATAATGATTTAGTAGCCAAATTATTAGGACACACAAATTCAAATATTACTCAAAAAACTTATATTGATTATGATAAAAGTATCATGGATGAGTATGCTATTTTAGCCATACAAGATCAAAAAATAGGCTTAAATTCTATGCAATTATTAAATATAAATAATAATTTAGAGCTTACAATTATCAAATTAGAGAAAGAAAATTGCAGCTTAAAAGAAGAAATTGAGATGCTAAAAAATAAAATAAAAAAACTAAAAAAATAATGTTTATCATATTTAAATAAAATTTTAAAAAATTCTGCATTTTACTAAATCCATTATAAAATAGCCACTTTGGTAAAATCAAATTAATAAAAATTGCTATTTAATAATGATAAAATAAAAATATTAATATTTATTATTAATAAAAATAATGCTATAATTATATTGAAATTAATTTAAATATATGTTTGCATTTTACTTATGCAACTATAAAAAAGGATTTTAAGGACATAATTATCGACATAATTTTCCTTATGTCCCGAAAATTTAAATTTTTTTATGTTAAATATAAGGAGACAACTATGAAAAATAGCACAAAAAAAATTGTAGCTTTAACAATGGGTATTTCTGCTGGGGTTGTTGGCGCAGTATCAGCCGGTACTTTAATTGGTTATGCAGTTGACCATTCAAACTATAAAAAAGAAAAATTGTGAATTGAAAACAGCATAAAATACTTTAAAGAATCAAATCAAAAATTAAAAGAAAAAGACAACAAATTAAAAGAAATTTGAAAGAAATATGAGAATCAAGAATGACTTTCTAGTCTTTGAAATGAACTAAGTTTGTGCCAAACTCAAATTGATTCAAATGAAAAACTTATTAAAGATTTACAGGGAATTATTGATCAACACTCTTCATATCATGTTGGCCGCCACTTTGGTAACAAAAGTTATATAAGAGCTGCTAAACACAGTAATGAAAAGAAAATATTTCAAAATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTTTTGATCTATAATTTGCTAATTGATTCCTATTTATCTAACAAGCTCTTTTTTAAATAATTCTTGTCAGTTTTTATAATTTGTTTTTCTTTTAATAGATTCATTAATTAATTTTTCAACTTCAAAATTTTTTATATCTAGTAAATAAGCTTCTCTTCTACTGAAGATGCCAACTTTTAGTAATCAATCTATTACTAATTCCATTTTTAAAGTTCTAGATTTCATATTAATAATTTCTTTGTATTCTTTTCTAGTCATATTTTCTCCTTTGTACTTATGCAGTGAATAAACAATTTAATATTTTCACTTGATTTATTAATTAATTTTTGAACTTCAGAATTTATTGGTGTTAAATTATATATTTTACTAAAATGACTGTTTTATAGTCTTTTTGGTTTATTAGACACTGATTTAATTGGTTTTTCTGATAACAATAACTTTTTGCAAAATACGCATTTAATCATTCTTCTTTTTTCTTGCTTCTAATTCATCATAGATTGCATCATGTAAAGAATTCGAGTAATGATAAAAATCTATTGTTGGTTTTCTTATATATGTTTCTTTTATATCTCATTCATTGCATTTATAAAAATTAACAGACAATTTAATTATTTTTAATCAAAATTCAATTGGTTTTATCAAATCAATTAATATAGAATTTTTTTTAAATAATGTCTTTACTTCTTTAGTTTCTTTTATATCATCTTCAATTCTATATGTTATGAAAATATATGATTGTTTTAAAAATTGTCAATGAGTATCATAATGTTCACAATAATCAGGTTTTTCAAGACAATCAATTACTTCATCAATAGCATTTATTAATGGCATTTCTAACATACCATCTATAAAATAATTGAAATCTTTTAATTCTTGCTCACTACAATTTTTTAATTTTTCACTTAAATTTGACATATTTCCTCCTTTTGCACTTATGCATCGAATAAACAATTTAATAATTTTTCACTTGATTCTTTATTATTTTTGTTAGGTAATGTCTGAAATGGAAATTGTGTGATATACAACTCTTTTTTATAATTAATTTTTTTGTTAAGATGATTAAATCCACAATCTAGTATGTAAATGATTTTAGATTTACCTTTACTATTTAATGATGTGAATAAAATTTTTTTATTTTCAAAATCTCATTCTATGAAAACTTCATTTTTGTTTTGAGTTTCCAGTAACTTCAAAATCTTAATATTGCCTTTATAAAAAGAACATATGGTGAATAGCGATTATTTTTTAATATTTCCTGGAAAATTATCTCAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGCTATTTAAATGACGAAAATAATTTGACAACTTCTATTCAATCTGTTATTAAAGAATTTTTAAATGCTTATAATTCAAATGTTGATTCAATCGAAACAACAATTAGATTAGCTAAACTAAATAACTCAAAACAAATTATTGATGAATTTGTACAAAAATCTAAAATTGAATTAAATTCTCTCAAATGAGATGAGTATATTGAAAGCAGAAACAAATTAATTAATGAATATGTTAATAGCAAAAATGAATTAGATTCAATAAAAAGTGGATTTAAAAAAGCATTTGTTAATATTAAATCAAGATTAAATGAATTTAATTTAGCATTAGAAGCAAACAAAACAACATTAAATGGATTTAATTTATTTGAAACTTCTTATAAATCAATAATTGATATACAAACTAAACTAGATCCTTTATTAAAAGCTAATTTAGATTCTGATATTGACAACAAAATTAATAATATTTCAGCATTCTTTGAAATAATTTCAAATCTTAAAAAATATGAAACAAATTATTTAGAAATATTTAAAATTATTAAAAACGCAACTGATTTAAACTTACAAAAAGTTAATAACTTTGCTTCAAAACCTAAAGAAATTTCAAAACTTATTAGTAATGCAAATCCAAAAATTGTTGATTTCTTATCAAAAGTTTTTGCTAATTTAAAAACATTAAGCCAATTTGAAGATGTTAAAAAAACAATTGAAGATATTGATAATAACAAACCTGATTTATCAACTGAAGAAATATCTTCAATTGAAAACAGTGAAATTCGAAATGACAAGGAAAAATTTAAAGGACTATTTGAAAAAATAAATTTAATCAATAATTTTATTTCATCGATTAATGAAAAAGATAAACTTTATAGTAATTTCTTAGAAAATGAAGCTCAATTTTGCTTAGAATCATACAATGAAATGGATGCGAAATTAGCAACAATGGCAAATGTGGAATGGACAGTTAATGAATATAAAAAGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACGATACAGATCTGCATCTATGACTGCTCGTGATTCTCTCATAAAACGCTTTCA", "length": 1004014, "complete": false, "type": "contig", "topology": "linear", "simple_id": "contig_1", "orig_id": "GCA_000209735.1|AP009608.1", "orig_description": "HMT-607 Mycoplasmopsis fermentans PG18" } ], "run": { "start": "2026-01-27 17:47:32", "end": "2026-01-27 17:55:31", "duration": "7.99 min" }, "version": { "bakta": "1.11.4", "db": { "version": "6.0", "type": "full" } } }