{ "genome": { "genus": "Mycoplasmoides", "species": "genitalium", "strain": "M6282", "taxon": "Mycoplasmoides genitalium M6282", "complete": true, "gram": "?", "translation_table": 11 }, "stats": { "size": 579504, "gc": 0.31671049725282313, "n_ratio": 0.0, "n50": 579504, "n90": 579504, "coding_ratio": 0.782831524890251 }, "features": [ { "type": "cds", "sequence": "contig_1", "start": 686, "stop": 1828, "strand": "+", "frame": 2, "gene": "dnaN", "product": "DNA polymerase III subunit beta", "db_xrefs": [ "COG:COG0592", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0005737", "GO:0006260", "GO:0006271", "GO:0008408", "GO:0009360", "KEGG:K02338", "RefSeq:WP_014894092.1", "SO:0001217", "UniParc:UPI00027B3517", "UniRef:UniRef100_UPI00027B3517", "UniRef:UniRef50_P47247", "UniRef:UniRef90_P47247" ], "nt": "ATGAAAATATTAATTAATAAAAGTGAATTGAATAAAATTTTGAAAAAAATGAATAACGTTATTATTTCCAATAACAAAATAAAACCACATCATTCATATTTTTTAATAGAGGCAAAAGAAAAAGAAATAAACTTTTATGCTAACAATGAATACTTTTCTGTCAAATGTAATTTAAATAAAAATATTGATATTCTTGAACAAGGCTCCTTAATTGTTAAAGGAAAAATTTTTAACGATCTTATTAATGGCATAAAAGAAGAGATTATTACTATTCAAGAAAAAGATCAAACACTTTTGGTTAAAACAAAAAAAACAAGTATTAATTTAAACACAATTAATGTGAATGAATTTCCAAGAATAAGGTTTAATGAAAAAAACGATTTAAGTGAATTTAATCAATTCAAAATAAATTATTCACTTTTAGTAAAAGGCATTAAAAAAATTTTTCACTCAGTTTCAAATAATCGTGAAATATCTTCTAAATTTAATGGAGTAAATTTCAATGGATCCAATGGAAAAGAAATATTTTTAGAAGCTTCTGACACTTATAAACTATCTGTTTTTGAGATAAAGCAAAAAACAGAACCATTTGATTTCATTTTGGAGAGTAATTTACTTAGTTTCATTAATTCTTTTAATCCTGAAGAAGATAAATCTATTGTTTTTTATTACAGAAAAGATAATAAAGATAGCTTTAGTACAGAAATGTTGATTTCAATGGATAACTTTATGATTAGTTACACATCGGTTAATGAAAAATTTCCAGAGGTAAACTACTTTTTTGAATTTGAACCTGAAACTAAAATAGTTGTTCAAAAAAATGAATTAAAAGATGCACTTCAAAGAATTCAAACTTTGGCTCAAAATGAAAGAACTTTTTTATGCGATATGCAAATTAACAGTTCTGAATTAAAAATAAGAGCTACTGTTAATAATATCGGAAATTCTCTTGAGGAAATTTCTTGTCTTAAATTTGAAGGTTATAAACTTAATATTTCTTTTAACCCAAGTTCTCTATTAGATCACATAGAGTCTTTTGAATCAAATGAAATAAATTTTGATTTCCAAGGAAATAGTAAGTATTTTTTGATAACCTCTAAAAGTGAACCTGAACTTAAGCAAATATTGGTTCCTTCAAGATAA", "aa": "MKILINKSELNKILKKMNNVIISNNKIKPHHSYFLIEAKEKEINFYANNEYFSVKCNLNKNIDILEQGSLIVKGKIFNDLINGIKEEIITIQEKDQTLLVKTKKTSINLNTINVNEFPRIRFNEKNDLSEFNQFKINYSLLVKGIKKIFHSVSNNREISSKFNGVNFNGSNGKEIFLEASDTYKLSVFEIKQKTEPFDFILESNLLSFINSFNPEEDKSIVFYYRKDNKDSFSTEMLISMDNFMISYTSVNEKFPEVNYFFEFEPETKIVVQKNELKDALQRIQTLAQNERTFLCDMQINSSELKIRATVNNIGNSLEEISCLKFEGYKLNISFNPSSLLDHIESFESNEINFDFQGNSKYFLITSKSEPELKQILVPSR", "aa_hexdigest": "12afbc4104b0dea6ea1c09897760a560", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3517", "ncbi_nrp_id": "WP_014894092.1", "uniref100_id": "UniRef100_UPI00027B3517", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3517", "RefSeq:WP_014894092.1", "UniRef:UniRef100_UPI00027B3517" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3517", "uniref90_id": "UniRef90_P47247" }, "psc": { "uniref90_id": "UniRef90_P47247", "gene": "dnaN", "product": "DNA polymerase III subunit beta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_P47247", "cog_id": "COG0592", "cog_category": "L", "kegg_orthology_id": "K02338", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0005737", "GO:0006260", "GO:0006271", "GO:0008408", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_P47247", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47247" ], "product": "Beta sliding clamp" }, "genes": [ "dnaN" ], "id": "KMADJIBOEG_43", "locus": "GCA000292445_00001" }, { "type": "cds", "sequence": "contig_1", "start": 1828, "stop": 2073, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00226.36" ], "nt": "ATGAATCTTTACGATCTTTTAGAACTACCAACTACAGCATCAATAAAAGAAATAAAAATTGCTTATAAAAGATTAGCAAAGCGTTATCACCCTGATGTAAATAAATTAGGTTCGCAAACTTTTGTTGAAATTAATAATGCTTATTCAATATTAAGTGATCCTAACCAAAAGGAAAAATATGATTCAATGCTGAAAGTTAATGATTTTCAAAATCGCATAAAAAATTTAGATATTAGTGTTAGATGA", "aa": "MNLYDLLELPTTASIKEIKIAYKRLAKRYHPDVNKLGSQTFVEINNAYSILSDPNQKEKYDSMLKVNDFQNRIKNLDISVR", "aa_hexdigest": "bd68886341465ed340ba720a06a2fb0d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00226.36", "name": "DnaJ", "length": 63, "aa_cov": 0.7407407407407407, "hmm_cov": 1.0, "evalue": 1.7874864924488404e-22, "score": 74.42524719238281, "start": 2, "stop": 61 } ], "seq_stats": { "molecular_weight": 9475.772099999996, "isoelectric_point": 9.221831703186034 }, "id": "KMADJIBOEG_44", "locus": "GCA000292445_00002" }, { "type": "cds", "sequence": "contig_1", "start": 2605, "stop": 2760, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGTAGTTAATAAAGTAAACAAAAGACTGCGTATTTTTTCAAGCTTTTTTGAGAACGATAAATCTAAATTATGGTTCCTTGTTCCAAACGATAAACAAAGTAATCCTAATAAGGGCGTTTTTAACTATAAAACTCAGCACTTTATTGATTAA", "aa": "MKVVNKVNKRLRIFSSFFENDKSKLWFLVPNDKQSNPNKGVFNYKTQHFID", "aa_hexdigest": "7ccff5a9fba33d568e382fcbb1e0eb7b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6148.016900000002, "isoelectric_point": 10.092283821105958 }, "id": "KMADJIBOEG_45", "locus": "GCA000292445_00003" }, { "type": "cds", "sequence": "contig_1", "start": 2845, "stop": 2979, "strand": "+", "frame": 1, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920FT50" ], "nt": "ATGGAAGAAAATAACAAAGCAAATATCTATGACTCTAGTAGCATTAAGGTCCTTGAAGGACTTGAGGCTGTTAGAAAACGCCCTGGAATGTACATTGGTTCTACTGGCGAAGAAGGTTTGCATCACATGATCTGA", "aa": "MEENNKANIYDSSSIKVLEGLEAVRKRPGMYIGSTGEEGLHHMI", "aa_hexdigest": "838391e1dd8186643f04d3bd2b89c52e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A920FT50", "query_cov": 0.9090909090909091, "subject_cov": 0.8, "identity": 0.7, "score": 58.5, "evalue": 1.34e-10, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0A920FT50" }, "pscc": { "uniref50_id": "UniRef50_A0A920FT50", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920FT50" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "KMADJIBOEG_46", "locus": "GCA000292445_00004" }, { "type": "cds", "sequence": "contig_1", "start": 3010, "stop": 3255, "strand": "+", "frame": 1, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380EBM1" ], "nt": "ATGGGAGGTTTTGCCAGTTTTGTTAAGCTTACCCTTGAAGATAATTTTGTTACCCGTGTAGAGGATGATGGAAGAGGGATACCTGTTGATATCCATCCTAAGACTAATCGTTCTACAGTTGAAACAGTTTTTACAGTTCTACACGCTGGCGGTAAATTTGATAACGATAGCTATAAAGTGTCAGGTGGTTTACACGGTGTTGGTGCATCAGTTGTTAATGCGCTTAGTTCTTCTTTTAAAGTTTGA", "aa": "MGGFASFVKLTLEDNFVTRVEDDGRGIPVDIHPKTNRSTVETVFTVLHAGGKFDNDSYKVSGGLHGVGASVVNALSSSFKV", "aa_hexdigest": "930d6e6912b2909e276c273e3dce4c94", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021B43CD8", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.568, "score": 93.2, "evalue": 2.53e-23, "valid": false, "product": "ATP-binding protein", "uniref50_id": "UniRef50_A0A380EBM1" }, "pscc": { "uniref50_id": "UniRef50_A0A380EBM1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380EBM1" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "KMADJIBOEG_47", "locus": "GCA000292445_00005" }, { "type": "cds", "sequence": "contig_1", "start": 3319, "stop": 3513, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTCCAAGAAGGTAACTCTGAAAAAGAGCATGGAACAATTGTTGAGTTTGTTCCTGATTTCTCTGTAATGGAAAAGAGTGATTACAAACAAACTGTAATTGTAAGCAGACTCCAGCAATTAGCTTTTTTAAACAAGGGAATAAGAATTGACTTTGTTGATAATCGTAAACAAAACCCACAGTCTTTTTCTTGA", "aa": "MVQEGNSEKEHGTIVEFVPDFSVMEKSDYKQTVIVSRLQQLAFLNKGIRIDFVDNRKQNPQSFS", "aa_hexdigest": "5dc3e325e8cc02961f18c22b3972012f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7404.266600000002, "isoelectric_point": 5.678067588806152 }, "id": "KMADJIBOEG_48", "locus": "GCA000292445_00006" }, { "type": "cds", "sequence": "contig_1", "start": 3529, "stop": 4557, "strand": "+", "frame": 1, "gene": "gyrB", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit B", "db_xrefs": [ "COG:COG0187", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0034335", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P22447", "UniRef:UniRef90_P22447" ], "nt": "TTGGTTGAATATATCCACCACCTAAACAACGAAAAAGAACCACTTTTTAATGAAGTTATTGCTGATGAAAAAACTGAAACTGTAAAAGCTGTTAATCGTGATGAAAACTACACAGTAAAGGTTGAAGTTGCTTTTCAATATAACAAAACATACAACCAATCAATTTTCAGTTTTTGTAACAACATTAATACTACAGAAGGTGGAACCCATGTGGAAGGTTTTCGTAATGCACTTGTTAAGATCATTAATCGCTTTGCTGTTGAAAATAAATTCCTAAAAGATAGTGATGAAAAGATTAACCGTGATGATGTTTGTGAAGGATTAACTGCTATTATTTCCATTAAACACCCAAACCCACAATATGAAGGACAAACTAAAAAGAAGTTAGGTAATACTGAGGTAAGACCTTTAGTTAATAGTGTTGTTAGTGAAATCTTTGAACGCTTCATGTTAGAAAACCCACAAGAAGCAAACGCTATCATCAGAAAAACACTTTTAGCTCAAGAAGCGAGAAGAAGAAGTCAAGAGGCTAGGGAGTTAACTCGTCGTAAATCACCTTTTGATAGTGGTTCATTACCAGGTAAATTAGCTGATTGTACAACCAGAGATCCTTCGATTAGTGAACTTTACATTGTTGAGGGTGATAGTGCTGGTGGCACTGCTAAAACAGGAAGAGATCGTTATTTTCAAGCTATCTTACCCTTAAGAGGAAAGATTTTAAACGTTGAAAAATCTAACTTTGAACAAATCTTTAATAATGCAGAAATTTCTGCATTAGTGATGGCAATAGGCTGTGGGATTAAACCTGATTTTGAACTTGAAAAACTTAGATATAGCAAGATTGTGATCATGACAGATGCTGATGTTGATGGTGCACACATAAGAACACTTCTCTTAACTTTCTTTTTTCGCTTTATGTATCCTTTGGTTGAACAAGGCAATATTTTTATTGCTCAACCCCCACTTTATAAAGTGTCATATTCCCATAAGGATTTATACATGCACACTGATGTTCAACTTGAACAGTGA", "aa": "MVEYIHHLNNEKEPLFNEVIADEKTETVKAVNRDENYTVKVEVAFQYNKTYNQSIFSFCNNINTTEGGTHVEGFRNALVKIINRFAVENKFLKDSDEKINRDDVCEGLTAIISIKHPNPQYEGQTKKKLGNTEVRPLVNSVVSEIFERFMLENPQEANAIIRKTLLAQEARRRSQEARELTRRKSPFDSGSLPGKLADCTTRDPSISELYIVEGDSAGGTAKTGRDRYFQAILPLRGKILNVEKSNFEQIFNNAEISALVMAIGCGIKPDFELEKLRYSKIVIMTDADVDGAHIRTLLLTFFFRFMYPLVEQGNIFIAQPPLYKVSYSHKDLYMHTDVQLEQ", "aa_hexdigest": "a401d9d9670848dc5b513361b388c93d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3D5R7J3", "query_cov": 1.0, "subject_cov": 0.8260869565217391, "identity": 0.5379999999999999, "score": 366.0, "evalue": 3.5e-122, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_K2BJ35" }, "genes": [ "gyrB" ], "truncated": "both", "pseudogene": { "start": 3229, "stop": 4797, "observations": { "mutation": [ 3253, 3511, 4555, 4633 ], "stop": [ 3253, 3511, 4555, 4633 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P22447", "query_cov": 1.0, "subject_cov": 0.5261538461538462, "identity": 0.997, "score": 670.0, "evalue": 2.79e-238, "gene_start": 1, "gene_end": 342, "reference_start": 229, "reference_end": 570, "reference_sequence": "MEENNKANIYDSSSIKVLEGLEAVRKRPGMYIGSTGEEGLHHMIWEIVDNSIDEAMGGFASFVKLTLEDNFVTRVEDDGRGIPVDIHPKTNRSTVETVFTVLHAGGKFDNDSYKVSGGLHGVGASVVNALSSSFKVWVFRQNKKYFLSFSDGGKVIGDLVQEGNSEKEHGTIVEFVPDFSVMEKSDYKQTVIVSRLQQLAFLNKGIRIDFVDNRKQNPQSFSWKYDGGLVEYIHHLNNEKEPLFNEVIADEKTETVKAVNRDENYTVKVEVAFQYNKTYNQSIFSFCNNINTTEGGTHVEGFRNALVKIINRFAVENKFLKDSDEKINRDDVCEGLTAIISIKHPNPQYEGQTKKKLGNTEVRPLVNSVVSEIFERFMLENPQEANAIIRKTLLAQEARRRSQEARELTRRKSPFDSGSLPGKLADCTTRDPSISELYIVEGDSAGGTAKTGRDRYFQAILPLRGKILNVEKSNFEQIFNNAEISALVMAIGCGIKPDFELEKLRYSKIVIMTDADVDGAHIRTLLLTFFFRFMYPLVEQGNIFIAQPPLYKVSYSHKDLYMHTDVQLEQWKSQNPNVKFGLQRYKGLGEMDALQLWETTMDPKVRTLLKVTVEDASIADKAFSLLMGDEVPPRREFIEKNARSVKNIDI" }, "paralog": false, "identity": 0.9923371647509579, "score": 1017.0, "evalue": 0.0, "description": "Internal stop codon at 3253, 3511, 4555, 4633. Nonsense mutation around 3253, 3511, 4555, 4633.", "psc": { "uniref90_id": "UniRef90_P22447", "gene": "gyrB", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit B", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P22447", "cog_id": "COG0187", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0034335", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P22447", "EC:5.6.2.2", "UniRef:UniRef50_P22447", "COG:COG0187", "COG:L", "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0034335", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P22447", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P22447" ], "product": "DNA gyrase subunit B" } }, "id": "KMADJIBOEG_49", "locus": "GCA000292445_00007" }, { "type": "cds", "sequence": "contig_1", "start": 4645, "stop": 4797, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCCTAAGGTTAGAACATTGTTAAAAGTTACTGTTGAAGATGCTTCTATTGCTGATAAAGCTTTTTCACTGTTGATGGGTGATGAAGTTCCCCCAAGAAGAGAATTTATTGAAAAAAATGCTCGTAGTGTTAAAAACATTGATATTTAA", "aa": "MDPKVRTLLKVTVEDASIADKAFSLLMGDEVPPRREFIEKNARSVKNIDI", "aa_hexdigest": "005320cf15fac2ee99e015a052764196", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A926IWG4", "query_cov": 1.0, "subject_cov": 0.8333333333333334, "identity": 0.68, "score": 68.2, "evalue": 3.33e-14, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0A926IWG4" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5657.521399999999, "isoelectric_point": 6.141760063171386 }, "id": "KMADJIBOEG_50", "locus": "GCA000292445_00008" }, { "type": "cds", "sequence": "contig_1", "start": 4812, "stop": 7322, "strand": "+", "frame": 3, "gene": "gyrA", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit A", "db_xrefs": [ "COG:COG0188", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0009330", "GO:0034335", "KEGG:K02469", "RefSeq:WP_009885559.1", "SO:0001217", "UniParc:UPI000012BE8C", "UniRef:UniRef100_P47250", "UniRef:UniRef50_P47719", "UniRef:UniRef90_P47250" ], "nt": "ATGGCAAAGCAACAAGATCAAGTAGATAAGATTCGTGAAAACTTAGACAATTCAACTGTCAAAAGTATTTCATTAGCAAATGAACTTGAGCGTTCATTCATGGAATATGCTATGTCAGTTATTGTTGCTCGTGCTTTACCTGATGCTAGAGATGGACTTAAACCAGTTCATCGTCGTGTTCTTTATGGTGCTTATATTGGTGGCATGCACCATGATCGTCCTTTTAAAAAGTCTGCGAGGATTGTTGGTGATGTAATGAGTAAATTCCACCCTCATGGTGATATGGCAATATATGACACCATGTCAAGAATGGCTCAAGACTTTTCATTAAGATACCTTTTAATTGATGGTCATGGTAATTTTGGTTCTATAGATGGTGATAGACCTGCTGCACAACGTTATACAGAAGCAAGATTATCTAAACTTGCAGCAGAACTTTTAAAAGATATTGATAAAGATACAGTTGACTTTATTGCTAATTATGATGGTGAGGAAAAAGAACCAACTGTTCTACCAGCAGCTTTCCCTAACTTACTTGCAAATGGTTCTAGTGGGATTGCAGTTGGAATGTCAACATCTATTCCTTCCCATAATCTCTCTGAATTAATTGCGGGTTTAATCATGTTAATTGATAATCCTCAATGCACTTTTCAAGAATTATTAACTGTAATTAAAGGACCTGATTTTCCAACAGGAGCTAACATTATCTACACAAAAGGAATTGAAAGCTACTTTGAAACAGGTAAAGGCAATGTAGTAATTCGTTCTAAAGTTGAGATAGAACAATTGCAAACAAGAAGTGCATTAGTTGTAACTGAAATTCCTTACATGGTTAACAAAACTACCTTAATTGAAAAGATTGTAGAACTTGTTAAAGCTGAAGAGATTTCAGGAATTGCTGATATCCGTGATGAATCCTCTCGAGAAGGAATAAGGTTAGTGATTGAAGTAAAACGCGACACTGTACCTGAAGTTTTATTAAATCAACTTTTTAAATCAACAAGATTACAAGTACGCTTCCCTGTTAATATGCTTGCTTTAGTTAAAGGAGCTCCTGTACTTCTCAACATGAAACAAGCTTTGGAAGTATATCTTGATCATCAAATTGATGTTCTTGTTAGAAAAACAAAGTTTGTGCTTAATAAACAACAAGAACGTTATCACATTTTAAGCGGACTTTTAATTGCTGCTTTAAATATTGATGAGGTTGTTGCAATTATTAAAAAATCAGCAAATAACCAGGAAGCAATTAATACATTAAATACAAAGTTTAAGCTTGATGAAATTCAAGCTAAAGCAGTTCTTGACATGCGTTTAAGGAGCTTAAGCGTACTTGAAGTTAACAAACTTCAAACTGAACAAAAAGAGTTAAAAGATTCAATTGAATTTTGTAAGAAAGTGTTAGCTGATCAAAAATTACAGCTAAAAATAATCAAAGAGGAATTGCAAAAAATCAATGATCAGTTTGGTGATGAAAGAAGAAGTGAAATTCTCTATGATATCTCTGAGGAAATTGATGATGAATCATTGATAAAAGTTGAGAATGTAGTGATAACTATGTCTACAAATGGTTATCTAAAAAGGATTGGAGTTGATGCTTATAATCTTCAACATCGTGGTGGAGTTGGGGTTAAAGGGCTAACTACTTATGTTGATGATAGTATTAGTCAATTATTGGTCTGTTCAACTCACTCTGACTTATTATTTTTTACTGATAAGGGTAAGGTTTATAGAATTAGAGCTCATCAAATTCCCTATGGTTTTAGAACAAATAAAGGTATTCCCGCTGTTAACTTAATCAAAATTGAAAAGGATGAAAGAATTTGTTCATTGTTATCTGTTAATAACTATGATGATGGTTATTTCTTTTTCTGTACTAAAAATGGAATTGTTAAAAGAACGAGCTTGAATGAATTCATCAACATCTTAAGTAATGGTAAGCGGGCTATATCTTTTGATGATAATGACACTTTGTATTCAGTAATTAAAACCCACGGAAATGATGAGATTTTTATTGGTTCTACCAATGGATTTGTTGTTCGATTCCATGAAAATCAACTCAGAGTTCTTTCAAGAACAGCAAGAGGTGTATTTGGTATCAGTTTAAATAAAGGAGAATTTGTTAATGGACTATCAACTTCAAGCAACGGTAGCTTACTTTTATCAGTCGGTCAAAATGGAATAGGTAAATTAACGAGCATAGATAAATATAGGCTCACAAAACGTAATGCTAAGGGAGTTAAAACTCTAAGGGTTACTGATAGAACAGGCCCTGTTGTTACAACAACCACTGTTTTTGGTAATGAGGATCTTTTAATGATTTCCTCTGCTGGTAAAATTGTGCGTACCAGTTTACAAGAACTTTCAGAACAAGGTAAAAACACTTCTGGTGTTAAGTTAATTAGATTAAAAGATAATGAACGTTTAGAAAGAGTAACTATCTTTAAAGAAGAGTTAGAAGACAAAGAAATGCAACTAGAAGATGTTGGATCCAAACAAATTACGCAATAA", "aa": "MAKQQDQVDKIRENLDNSTVKSISLANELERSFMEYAMSVIVARALPDARDGLKPVHRRVLYGAYIGGMHHDRPFKKSARIVGDVMSKFHPHGDMAIYDTMSRMAQDFSLRYLLIDGHGNFGSIDGDRPAAQRYTEARLSKLAAELLKDIDKDTVDFIANYDGEEKEPTVLPAAFPNLLANGSSGIAVGMSTSIPSHNLSELIAGLIMLIDNPQCTFQELLTVIKGPDFPTGANIIYTKGIESYFETGKGNVVIRSKVEIEQLQTRSALVVTEIPYMVNKTTLIEKIVELVKAEEISGIADIRDESSREGIRLVIEVKRDTVPEVLLNQLFKSTRLQVRFPVNMLALVKGAPVLLNMKQALEVYLDHQIDVLVRKTKFVLNKQQERYHILSGLLIAALNIDEVVAIIKKSANNQEAINTLNTKFKLDEIQAKAVLDMRLRSLSVLEVNKLQTEQKELKDSIEFCKKVLADQKLQLKIIKEELQKINDQFGDERRSEILYDISEEIDDESLIKVENVVITMSTNGYLKRIGVDAYNLQHRGGVGVKGLTTYVDDSISQLLVCSTHSDLLFFTDKGKVYRIRAHQIPYGFRTNKGIPAVNLIKIEKDERICSLLSVNNYDDGYFFFCTKNGIVKRTSLNEFINILSNGKRAISFDDNDTLYSVIKTHGNDEIFIGSTNGFVVRFHENQLRVLSRTARGVFGISLNKGEFVNGLSTSSNGSLLLSVGQNGIGKLTSIDKYRLTKRNAKGVKTLRVTDRTGPVVTTTTVFGNEDLLMISSAGKIVRTSLQELSEQGKNTSGVKLIRLKDNERLERVTIFKEELEDKEMQLEDVGSKQITQ", "aa_hexdigest": "f92bc4b44c4f2d5989364788f6845074", "start_type": "ATG", "rbs_motif": "ATTTA", "ups": { "uniparc_id": "UPI000012BE8C", "ncbi_nrp_id": "WP_009885559.1", "uniref100_id": "UniRef100_P47250", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012BE8C", "RefSeq:WP_009885559.1", "UniRef:UniRef100_P47250" ] }, "ips": { "uniref100_id": "UniRef100_P47250", "uniref90_id": "UniRef90_P47250" }, "psc": { "uniref90_id": "UniRef90_P47250", "gene": "gyrA", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit A", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P47719", "cog_id": "COG0188", "cog_category": "L", "kegg_orthology_id": "K02469", "go_ids": [ "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0009330", "GO:0034335" ] }, "pscc": { "uniref50_id": "UniRef50_P47719", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47719" ], "product": "DNA gyrase subunit A" }, "genes": [ "gyrA" ], "id": "KMADJIBOEG_51", "locus": "GCA000292445_00009" }, { "type": "cds", "sequence": "contig_1", "start": 7294, "stop": 7857, "strand": "+", "frame": 1, "gene": "serS", "product": "serine--tRNA ligase", "db_xrefs": [ "COG:COG0172", "COG:J", "EC:6.1.1.11", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0005737", "GO:0006434", "GO:0016260", "KEGG:K01875", "SO:0001217", "UniRef:UniRef50_P47251", "UniRef:UniRef90_P47251" ], "nt": "ATGTTGGATCCAAACAAATTACGCAATAACTATGATTTCTTTAAAAAGAAACTGTTAGAAAGAAATGTAAATGAGCAATTATTAAATCAGTTTATTCAAACTGATAAACTAATGCGCAAAAACTTACAACAACTTGAACTTGCTAACCAAAAACAAAGCTTGTTGGCAAAACAAGTTGCTAAGCAAAAAGATAATAAAAAGCTATTAGCTGAATCAAAAGAACTTAAGCAGAAGATTGAAAACTTAAATAGTGCTTATAAAGATTCACAAAACATTAGTCAGGATTTACTTCTAAATTTTCCTAATATTGCTCATGAATCAGTTCCTGTTGGTAAAAATGAATCAGCAAACTTAGAACTCCTTAAAGAAGGGAGAAAACCAGTTTTTGATTTCAAACCTTTACCACATTGGGAGTTATGTGAAAAGTTAAATTTAGTTGCTTTTGATAAAGCTACTAAGATTAGTGGAACTAGGTTTGTTGCATATACAGATAAAGCAGCTAAACTGCTTAGAGCGATAACTAATCTAATGATTGACCTTAATAAAAGCAAGTATCAAGAATGA", "aa": "MLDPNKLRNNYDFFKKKLLERNVNEQLLNQFIQTDKLMRKNLQQLELANQKQSLLAKQVAKQKDNKKLLAESKELKQKIENLNSAYKDSQNISQDLLLNFPNIAHESVPVGKNESANLELLKEGRKPVFDFKPLPHWELCEKLNLVAFDKATKISGTRFVAYTDKAAKLLRAITNLMIDLNKSKYQE", "aa_hexdigest": "a4a983d104cb3c2bd0ef6dccfffcae96", "start_type": "ATG", "rbs_motif": null, "genes": [ "serS" ], "truncated": "3-prime", "pseudogene": { "start": 7294, "stop": 8160, "observations": { "mutation": [ 7855 ], "stop": [ 7855 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47251", "query_cov": 1.0, "subject_cov": 0.44844124700239807, "identity": 0.9890000000000001, "score": 345.0, "evalue": 1.46e-116, "gene_start": 1, "gene_end": 187, "reference_start": 1, "reference_end": 187, "reference_sequence": "MLDPNKLRNNYDFFKKKLLERNVNEQLLNQFIQTDKLMRKNLQQLELANQKQSLLAKQVAKQKDNKKLLAESKELKQKIENLNNAYKDSQNISQDLLLNFPNIAHESVPVGKNESANLELLKEGRKPVFDFKPLPHRELCEKLNLVAFDKATKISGTRFVAYTDKAAKLLRAITNLMIDLNKSKYQEWNLPVVINELSLRSTGQLPKFKDDVFKLENTRYYLSPTLEVQLINLHANEIFNEEDLPKYYTATGINFRQEAGSAGKQTKGTIRLHQFQKTELVKFCKPENAINELEAMVRDAEQILKALKLPFRRLLLCTGDMGFSAEKTYDLEVWMAASNEYREVSSCSSCGDFQARRAMIRYKDINNGKNSYVATLNGTALSIDRIFAAILENFQTKDGKILIPQALKKYLDFDTIK" }, "paralog": false, "identity": 0.9895833333333334, "score": 560.0, "evalue": 1.16e-203, "description": "Internal stop codon at 7855. Nonsense mutation around 7855.", "psc": { "uniref90_id": "UniRef90_P47251", "gene": "serS", "product": "serine--tRNA ligase", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_P47251", "cog_id": "COG0172", "cog_category": "J", "kegg_orthology_id": "K01875", "go_ids": [ "GO:0000166", "GO:0004828", "GO:0005524", "GO:0005737", "GO:0006434", "GO:0016260" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47251", "EC:6.1.1.11", "UniRef:UniRef50_P47251", "COG:COG0172", "COG:J", "KEGG:K01875", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0005737", "GO:0006434", "GO:0016260" ] }, "pscc": { "uniref50_id": "UniRef50_P47251", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47251" ], "product": "Serine--tRNA ligase" } }, "id": "KMADJIBOEG_52", "locus": "GCA000292445_00010" }, { "type": "cds", "sequence": "contig_1", "start": 8107, "stop": 8295, "strand": "+", "frame": 1, "gene": null, "product": "aminoacyl--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0032988E16" ], "nt": "TTGCATCAGTTTCAAAAAACTGAGTTAGTTAAGTTTTGTAAACCTGAAAATGCTATCAATGAATTGGAAGCAATGGTTAGAGATGCTGAACAAATCTTAAAGGCACTTAAGTTACCTTTTAGAAGGTTATTGTTATGTACTGGTGATATGGGCTTTAGTGCTGAAAAAACATATGATCTTGAAGTTTGA", "aa": "MHQFQKTELVKFCKPENAINELEAMVRDAEQILKALKLPFRRLLLCTGDMGFSAEKTYDLEV", "aa_hexdigest": "e28a48f94f7fd7ff0c03f1b7e954a7db", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002F2627E6", "query_cov": 1.0, "subject_cov": 0.8857142857142857, "identity": 0.5479999999999999, "score": 73.6, "evalue": 5.18e-16, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_UPI0032988E16", "kegg_orthology_id": "K01875" }, "pscc": { "uniref50_id": "UniRef50_UPI0032988E16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0032988E16" ], "product": "aminoacyl--tRNA ligase-related protein" }, "genes": [], "id": "KMADJIBOEG_53", "locus": "GCA000292445_00011" }, { "type": "cds", "sequence": "contig_1", "start": 8296, "stop": 8547, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "ATGGCAGCTAGCAATGAATATCGTGAAGTTTCTTCTTGTTCATCTTGTGGTGATTTTCAAGCAAGAAGAGCTATGATTCGTTACAAAGATATTAACAACGGTAAAAACAGTTATGTTGCTACTTTAAATGGAACAGCATTATCTATTGATAGAATTTTTGCTGCAATTCTAGAAAATTTTCAAACAAAAGATGGCAAAATTCTTATCCCACAAGCATTAAAAAAATACCTTGATTTTGACACAATCAAGTAA", "aa": "MAASNEYREVSSCSSCGDFQARRAMIRYKDINNGKNSYVATLNGTALSIDRIFAAILENFQTKDGKILIPQALKKYLDFDTIK", "aa_hexdigest": "dcc1f77d2836fa16e7d8406aa61056ff", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00345BB37E", "query_cov": 0.891566265060241, "subject_cov": 0.8222222222222222, "identity": 0.5539999999999999, "score": 89.4, "evalue": 1.17e-21, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_A0A3B8WPJ3", "kegg_orthology_id": "K01875" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 57, "aa_cov": 0.6867469879518072, "hmm_cov": 0.9649122807017544, "evalue": 2.1411441160651048e-09, "score": 32.797603607177734, "start": 4, "stop": 60 } ], "seq_stats": { "molecular_weight": 9314.53189999999, "isoelectric_point": 9.001543235778808 }, "id": "KMADJIBOEG_54", "locus": "GCA000292445_00012" }, { "type": "cds", "sequence": "contig_1", "start": 8551, "stop": 9183, "strand": "+", "frame": 1, "gene": "tmk", "product": "dTMP kinase", "db_xrefs": [ "COG:COG0125", "COG:F", "EC:2.7.4.9", "GO:0004798", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235", "KEGG:K00943", "RefSeq:WP_009885557.1", "SO:0001217", "UniParc:UPI0000032EAD", "UniRef:UniRef100_P47252", "UniRef:UniRef50_P47252", "UniRef:UniRef90_P47252" ], "nt": "ATGAATAAAGGTGTTTTTGTTGTTATTGAAGGAGTTGATGGAGCGGGCAAAACTGCTTTAATTGAAGGTTTTAAAAAACTTTATCCAACTAAGTTTTTGAACTATCAACTTACTTATACTAGAGAACCTGGTGGTACTTTGTTAGCTGAAAAAATTCGTCAACTTCTTTTAAATGAAACAATGGAACCTCTAACTGAAGCTTATTTGTTTGCCGCAGCTAGAACTGAACATATCAGTAAGCTAATTAAACCAGCAATTGAAAAAGAACAACTAGTTATTTCAGATAGATTTGTTTTCTCTAGTTTTGCATACCAAGGATTAAGCAAAAAAATAGGCATTGATACAGTAAAACAGATTAATCATCATGCGTTAAGAAATATGATGCCAAACTTTACCTTTATTTTGGATTGCAATTTTAAAGAAGCATTACAAAGGATGCAAAAGCGTGGTAATGATAATCTTCTTGATGAATTTATTAAAGGAAAGAATGATTTTGATACAGTTCGTTCTTATTATTTAAGCTTAGTTGATAAAAAAAACTGTTTCTTGATTAATGGTGATAATAAACAAGAACACCTAGAGAAATTTATTGAATTGTTAACAAGATGCTTACAACAACCCACGCATTACTAA", "aa": "MNKGVFVVIEGVDGAGKTALIEGFKKLYPTKFLNYQLTYTREPGGTLLAEKIRQLLLNETMEPLTEAYLFAAARTEHISKLIKPAIEKEQLVISDRFVFSSFAYQGLSKKIGIDTVKQINHHALRNMMPNFTFILDCNFKEALQRMQKRGNDNLLDEFIKGKNDFDTVRSYYLSLVDKKNCFLINGDNKQEHLEKFIELLTRCLQQPTHY", "aa_hexdigest": "b0628416374409bfb1dc17ac3d82a808", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000032EAD", "ncbi_nrp_id": "WP_009885557.1", "uniref100_id": "UniRef100_P47252", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000032EAD", "RefSeq:WP_009885557.1", "UniRef:UniRef100_P47252" ] }, "ips": { "uniref100_id": "UniRef100_P47252", "uniref90_id": "UniRef90_P47252" }, "psc": { "uniref90_id": "UniRef90_P47252", "gene": "tmk", "product": "dTMP kinase", "ec_ids": [ "2.7.4.9" ], "uniref50_id": "UniRef50_P47252", "cog_id": "COG0125", "cog_category": "F", "kegg_orthology_id": "K00943", "go_ids": [ "GO:0004798", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235" ] }, "pscc": { "uniref50_id": "UniRef50_P47252", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47252" ], "product": "Thymidylate kinase" }, "genes": [ "tmk" ], "id": "KMADJIBOEG_55", "locus": "GCA000292445_00013" }, { "type": "cds", "sequence": "contig_1", "start": 9156, "stop": 9323, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTACAACAACCCACGCATTACTAATCATTCAAAGAAAAGGTAGTTTCTTAAAACCTTTTCTTGATAATTATCTTACTAGTATTGTTTGTGAAAACAAAAATGGTTGCAAAAAGTGTATAAACTGTTTGGAAATTCTCAATAATAAATACAACAGCTTATATTGA", "aa": "MLTTTHALLIIQRKGSFLKPFLDNYLTSIVCENKNGCKKCINCLEILNNKYNSLY", "aa_hexdigest": "eaef039d4c156b9a8950d9e6f9012a48", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6339.452299999999, "isoelectric_point": 9.046606636047361 }, "id": "KMADJIBOEG_56", "locus": "GCA000292445_00014" }, { "type": "cds", "sequence": "contig_1", "start": 9468, "stop": 9755, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGACTAGTTGAAGATAGTCCGATAAATAGTTATGGTATTTTTACAACTAAAAATGAAAGTTTAATTCTTTCCACTTTTTTAAGTAGAGTACAGAAAGTAGTTTTAAAAAAAGCTAGTAAAGTTCCTTTTAAAGTAAGCAAAAATGATCAAGAAATTATTACAAGTTTTTTTACTGTAGATGAACAAATTGAAGCAATTGAAAATGGAAGTTTTAACCGTTTCAAAATTATCTTAGATGCATGTTTAAACAAAAAAACAGGTACAGAACAAATTTATCATGCTTGA", "aa": "MRLVEDSPINSYGIFTTKNESLILSTFLSRVQKVVLKKASKVPFKVSKNDQEIITSFFTVDEQIEAIENGSFNRFKIILDACLNKKTGTEQIYHA", "aa_hexdigest": "1ad9da980b4fbfcd8b0643c6465d8fab", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10816.33169999999, "isoelectric_point": 8.75959339141846 }, "id": "KMADJIBOEG_57", "locus": "GCA000292445_00015" }, { "type": "cds", "sequence": "contig_1", "start": 9923, "stop": 10804, "strand": "+", "frame": 2, "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "db_xrefs": [ "COG:COG0486", "COG:J", "EC:3.6.-.-", "GO:0002098", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0006400", "GO:0030488", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47254", "UniRef:UniRef90_P47254" ], "nt": "ATGAAAAGCGAAATTAATATTTTTGCACTAGCAACTGCACCTTTTAATAGTGCATTACATATTATTAGGTTTTCTGGTCCTGATGTTTATGAGATTTTAAACAAGATAACTAATAAAAAAATAACAAGAAAAGGGATGCAAATTCAACGCACATGGATAGTTGATGAAAACAATAAGCGAATTGATGATGTGCTATTATTTAAATTTGTCTCTCCAAATTCTTATACAGGAGAAGATTTAATTGAAATTTCTTGTCATGGTAACATGTTGATCGTTAATGAAATTTGCGCACTTCTTTTAAAAAAAGGAGGTGTTTATGCCAAACCTGGTGAATTTACCCAAAGGAGTTTTTTAAATGGAAAAATGAGTTTACAACAAGCTAGTGCTGTAAATAAATTGATTTTATCTCCTAACTTATTAGTTAAAGATATAGTCTTAAATAATTTAGCGGGTGAAATGGATCAACAATTAGAACAAATAGCTCAACAAGTTAATCAATTAGTAATGCAAATGGAAGTAAACATTGATTATCCAGAATATCTTGATGAACAAGTAGAACTATCAACTTTAAATAATAAAGTTAAATTGATTATTGAAAAGCTTAAAAGAATTATTGAAAATAGTAAACAACTCAAAAAACTTCACGATCCTTTTAAAATTGCCATTATAGGCGAAACTAATGTAGGTAAATCTTCTTTACTCAACGCTTTATTAAATCAAGATAAAGCGATAGTTTCAAATATTAAAGGTAGTACACGCGATGTTGTTGAAGGGGATTTCAATTTAAATGGTTATTTAATCAAGATCTTAGATACTGCAGGTATCCGTAAACATAAAAGTGGGCTTGAAAAAGCAGGAATTAAAAAAGCTTTGAATCTATAA", "aa": "MKSEINIFALATAPFNSALHIIRFSGPDVYEILNKITNKKITRKGMQIQRTWIVDENNKRIDDVLLFKFVSPNSYTGEDLIEISCHGNMLIVNEICALLLKKGGVYAKPGEFTQRSFLNGKMSLQQASAVNKLILSPNLLVKDIVLNNLAGEMDQQLEQIAQQVNQLVMQMEVNIDYPEYLDEQVELSTLNNKVKLIIEKLKRIIENSKQLKKLHDPFKIAIIGETNVGKSSLLNALLNQDKAIVSNIKGSTRDVVEGDFNLNGYLIKILDTAGIRKHKSGLEKAGIKKALNL", "aa_hexdigest": "3abac357846f16cb5a0b9bbe874ddde6", "start_type": "ATG", "rbs_motif": "ATTTA", "genes": [ "mnmE" ], "truncated": "3-prime", "pseudogene": { "start": 9923, "stop": 11106, "observations": { "deletion": [ 10787 ], "stop": [ 11057 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47254", "query_cov": 0.9897610921501706, "subject_cov": 0.6561085972850679, "identity": 0.997, "score": 553.0, "evalue": 4.33e-196, "gene_start": 1, "gene_end": 290, "reference_start": 1, "reference_end": 290, "reference_sequence": "MKSEINIFALATAPFNSALHIIRFSGPDVYEILNKITNKKITRKGMQIQRTWIVDENNKRIDDVLLFKFVSPNSYTGEDLIEISCHGNMLIVNEICALLLKKGGVYAKPGEFTQRSFLNGKMSLQQASAVNKLILSPNLLVKDIVLNNLAGEMDQQLEQIAQQVNQLVMQMEVNIDYPEYLDEQVELSTLNNKVKLIIEKLKRIIENSKQLKKLHDPFKIAIIGETNVGKSSLLNALLNQDKAIVSNIKGSTRDVVEGDFNLNGYLIKILDTAGIRKHKSGLEKAGIKKSFESIKQANLVIYLLDATHPKKDLELISFFKKNKKDFFVFYNKKDLITNKFENSISAKQKDIKELVDLLTKYINEFYKKIDQKIYLIENWQQILIEKIKEQLEQFLKQQKKYLFFDVLVTHLREAQQDILKLLGKDVGFDLVNEIFNNFCLGK" }, "paralog": false, "identity": 0.9949238578680203, "score": 753.0, "evalue": 1.47e-277, "description": "Internal stop codon at 11057. Frameshift due to deletion around 10787.", "psc": { "uniref90_id": "UniRef90_P47254", "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "ec_ids": [ "3.6.-.-" ], "uniref50_id": "UniRef50_P47254", "cog_id": "COG0486", "cog_category": "J", "go_ids": [ "GO:0002098", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0006400", "GO:0030488", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47254", "EC:3.6.-.-", "UniRef:UniRef50_P47254", "COG:COG0486", "COG:J", "GO:0002098", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0006400", "GO:0030488", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47254", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47254" ], "product": "tRNA modification GTPase MnmE" } }, "id": "KMADJIBOEG_58", "locus": "GCA000292445_00016" }, { "type": "cds", "sequence": "contig_1", "start": 10816, "stop": 11058, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTATTTATCTTTTAGATGCAACACATCCAAAGAAAGATCTTGAATTAATTAGTTTTTTTAAGAAAAATAAAAAGGATTTTTTTGTTTTCTATAACAAAAAAGATTTAATTACAAATAAGTTTGAAAATAGTATTTCTGCAAAGCAAAAAGATATTAAAGAATTAGTTAATTTATTAACTAAATATATTAACGAGTTTTATAAAAAAATAGATCAAAAAATCTATCTGATTGAAAATTGA", "aa": "MVIYLLDATHPKKDLELISFFKKNKKDFFVFYNKKDLITNKFENSISAKQKDIKELVNLLTKYINEFYKKIDQKIYLIEN", "aa_hexdigest": "ee69a5fc9b15ab6f4f1eb634a0499752", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9712.305399999996, "isoelectric_point": 9.372236442565917 }, "id": "KMADJIBOEG_59", "locus": "GCA000292445_00017" }, { "type": "cds", "sequence": "contig_1", "start": 11250, "stop": 11891, "strand": "+", "frame": 3, "gene": "tatD", "product": "putative metal-dependent hydrolase MG009", "db_xrefs": [ "COG:COG0084", "COG:N", "EC:3.1.-.-", "GO:0004536", "GO:0005829", "GO:0046872", "KEGG:K03424", "SO:0001217", "UniRef:UniRef50_P47255", "UniRef:UniRef90_P47255" ], "nt": "ATGGAATACTTTGATGCACATTGTCATTTAAATTGTGAACCTTTACTGAGTGAAATTGAAAAAAGCATCGCTAATTTCAAATTAATTAATTTAAAAGCAAATGTTGTAGGTACAGATTTGGATAATTCTAAAATTGCTGTTGAATTAGCTAAAAAATATCCTGATCTTTTAAAAGCAACCATAGGTATCCATCCAAATGATGTTCATTTAGTTGATTTTAAAAAGACAAAAAAACAACTTAATGAACTATTAATAAATAACAGAAATTTCATAAGTTGTATTGGTGAATATGGTTTTGATTATCACTACACAACAGAATTTATTGAATTGCAAAACAAATTCTTTGAGATGCAATTTGAAATAGCTGAAACTAATAAATTGGTTCACATGCTTCATATTCGTGATGCTCATGAAAAAATTTATGAAATATTAACAAGATTAAAGCCAACTCAACCTGTGATTTTTCATTGTTTCAGTCAAGATATAAATATTGCTAAAAAGCTACTATCATTAAAAGATTTAAATATTGACATCTTCTTTTCTATCCCAGGGATAGTTACTTTTAAGAATGCTCAAGCATTACATGAAGCTTTAAAGATTATTCCTAGTGAATTACTTTTAAGTGAAACTGACTCACCGTGA", "aa": "MEYFDAHCHLNCEPLLSEIEKSIANFKLINLKANVVGTDLDNSKIAVELAKKYPDLLKATIGIHPNDVHLVDFKKTKKQLNELLINNRNFISCIGEYGFDYHYTTEFIELQNKFFEMQFEIAETNKLVHMLHIRDAHEKIYEILTRLKPTQPVIFHCFSQDINIAKKLLSLKDLNIDIFFSIPGIVTFKNAQALHEALKIIPSELLLSETDSP", "aa_hexdigest": "fe4aaaf19581833bce1f838a004b8d5f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47255", "query_cov": 1.0, "subject_cov": 0.8129770992366412, "identity": 1.0, "score": 427.0, "evalue": 1.68e-150, "valid": true, "gene": "TatD", "product": "Uncharacterized metal-dependent hydrolase MG009", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P47255", "cog_id": "COG0084", "cog_category": "N", "kegg_orthology_id": "K03424", "go_ids": [ "GO:0005829", "GO:0004536", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47255", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47255" ], "product": "Uncharacterized metal-dependent hydrolase MG009" }, "genes": [ "tatD" ], "id": "KMADJIBOEG_60", "locus": "GCA000292445_00018" }, { "type": "cds", "sequence": "contig_1", "start": 12067, "stop": 12243, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGTTAGAACTGAAAGATTAAACGAATTGTTTTTTGTTTATCATAAAAACTTAAAAAATCAATCTAAATCTAAATATAGCAGAGCAGTTAATTACTTAAAAAGGCGTGGATTTAATTTACAAGATTTTTTAAAAGTTGGGGGTGGTGTTGGTTATTTACAAAATAAAGAATGA", "aa": "MDVRTERLNELFFVYHKNLKNQSKSKYSRAVNYLKRRGFNLQDFLKVGGGVGYLQNKE", "aa_hexdigest": "a751731d7e098c9c81701eadb454df60", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6898.821600000003, "isoelectric_point": 10.070106697082519 }, "id": "KMADJIBOEG_61", "locus": "GCA000292445_00019" }, { "type": "cds", "sequence": "contig_1", "start": 12507, "stop": 12656, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAAAACAAAAAAGATTAAAAAGATTTTTATCTGTTTTGATAATGATTTAGCTGGAAAGAATGGAGCAAAAAATTTAAAAGAATATCTAACTAAGCAAGGATTTATAACAAAAGTTATAGAAATTAAAGCTGCCGCAAAAGACTGA", "aa": "MIKTKKIKKIFICFDNDLAGKNGAKNLKEYLTKQGFITKVIEIKAAAKD", "aa_hexdigest": "f8ee1304f8c4dd7a2f89a75ccb2b2028", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5529.605700000002, "isoelectric_point": 9.81474742889404 }, "id": "KMADJIBOEG_62", "locus": "GCA000292445_00020" }, { "type": "cds", "sequence": "contig_1", "start": 13102, "stop": 13329, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAATGTTTGATCTCGTCAGCACTATTAACTAAATGTACATCTTTTCCACCGTGTGAATTACGTTTCTTAACGATGGCAGGAAATGATTTGATTGTTTCTTTACTAAGAAGAGAAGAATTGACAGTTAGAAATCTATTTTGTTTTAATCATTTATATGTTTCGTATTTATCGTTTGCTATAAAAACAACATTAAAAGGATTAACTAAAAAAGTATTTATTTGA", "aa": "MFKCLISSALLTKCTSFPPCELRFLTMAGNDLIVSLLRREELTVRNLFCFNHLYVSYLSFAIKTTLKGLTKKVFI", "aa_hexdigest": "0b51cb255ccac4ecfde7fe44c2e2d356", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8610.31359999999, "isoelectric_point": 9.505879402160645 }, "id": "KMADJIBOEG_63", "locus": "GCA000292445_00021" }, { "type": "cds", "sequence": "contig_1", "start": 13442, "stop": 13561, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAAAATTAAATTAAAAAACAGAAAAGCATTAGTTGTTTATGACAACAAGGATGATTTTGAAAAAAATCAAACATTTGCTTTAAGTCTAATCAAGGAATTACAAAAGAAAAACTAA", "aa": "MGKIKLKNRKALVVYDNKDDFEKNQTFALSLIKELQKKN", "aa_hexdigest": "c914e02b13f6530b4262049df691fc7b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4610.381, "isoelectric_point": 9.822999382019042 }, "id": "KMADJIBOEG_64", "locus": "GCA000292445_00022" }, { "type": "cds", "sequence": "contig_1", "start": 13566, "stop": 14054, "strand": "-", "frame": 2, "gene": "lysX", "product": "putative protein MG012", "db_xrefs": [ "COG:COG0189", "COG:EHJQ", "GO:0005524", "GO:0005737", "GO:0009432", "GO:0018169", "GO:0036211", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P75097", "UniRef:UniRef90_P47258" ], "nt": "ATGCTCGATGTTTTTATTAATCAAATAAAACAAAGTATGGAGTTTCCTGTTATTGTTAAAAGTGTTTTTGGTTCTTTTGGTGATTATGTTTTTTTGTGTTTAGATGAACAAAAATTAAGAAAAACTTTAATGTCTTTTAATCAACAAGCAATTGTGCAAAAATACATTACTTGCTCTAAAGGTGAATCGGTAAGAGTTATTGTTGTGAACAATAAAGTTATAGGTGCTTTACATACAACTAATAATAGTGATTTTCGTTCTAATCTCAATAAAGGGGCAAAGGCAGAACGCTTTTTTTTGAATAAGGAACAAGAAAATTTAGCAGTTAAAATTAGTAAAGTAATGCAACTTTTTTATTGCGGTATTGATTTTTTGTTTGATCAAGACAGATCATTGATCTTTTGTGAAGTAAATCCTAATGTGCAATTAACAAGAAGCTCAATGTATTTAAATACTAATCTTGCAATTGAGCTTTTAAAAGCAATTTAG", "aa": "MLDVFINQIKQSMEFPVIVKSVFGSFGDYVFLCLDEQKLRKTLMSFNQQAIVQKYITCSKGESVRVIVVNNKVIGALHTTNNSDFRSNLNKGAKAERFFLNKEQENLAVKISKVMQLFYCGIDFLFDQDRSLIFCEVNPNVQLTRSSMYLNTNLAIELLKAI", "aa_hexdigest": "e7242341018933fd7eacc87b25ad2c9f", "start_type": "ATG", "rbs_motif": null, "genes": [ "lysX" ], "truncated": "3-prime", "pseudogene": { "start": 13566, "stop": 14354, "observations": { "mutation": [ 14066, 14216 ], "stop": [ 14066, 14216 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47258", "query_cov": 1.0, "subject_cov": 0.5644599303135889, "identity": 1.0, "score": 316.0, "evalue": 2.55e-107, "gene_start": 1, "gene_end": 162, "reference_start": 126, "reference_end": 287, "reference_sequence": "MKKINVVYNPAFNPISSKLNQTQLLKNASEELDIELKFFTSFDINTTKAKANLPFISNKILFMDKNIALARWLESNGFEVINSSIGINNADNKGLSHAIIAQYPFIKQIKTLLGPQNFDREWNPVMLDVFINQIKQSMEFPVIVKSVFGSFGDYVFLCLDEQKLRKTLMSFNQQAIVQKYITCSKGESVRVIVVNNKVIGALHTTNNSDFRSNLNKGAKAERFFLNKEQENLAVKISKVMQLFYCGIDFLFDQDRSLIFCEVNPNVQLTRSSMYLNTNLAIELLKAI" }, "paralog": false, "identity": 0.9923664122137404, "score": 506.0, "evalue": 7.91e-185, "description": "Internal stop codon at 14066, 14216. Nonsense mutation around 14066, 14216.", "psc": { "uniref90_id": "UniRef90_P47258", "gene": "LysX", "product": "Uncharacterized protein MG012", "uniref50_id": "UniRef50_P75097", "cog_id": "COG0189", "cog_category": "EHJQ", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0046872", "GO:0018169", "GO:0036211", "GO:0009432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47258", "UniRef:UniRef50_P75097", "COG:COG0189", "COG:EHJQ", "GO:0005737", "GO:0005524", "GO:0046872", "GO:0018169", "GO:0036211", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P75097", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75097" ], "product": "Uncharacterized protein MG012 homolog" } }, "id": "KMADJIBOEG_65", "locus": "GCA000292445_00023" }, { "type": "cds", "sequence": "contig_1", "start": 14214, "stop": 14429, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATAAACGTTGTTTACAATCCAGCATTTAATCAAATTAGCTCTAAATTAAATCAAACTCAACTTTTAAAAAATGCTAGTGAAGAGTTAGATATAGAACTAAAATTCTTTACTAGTTTTGATATTAATACAACTAAAGCAAAAGCAAATTTACCCTTCATATCCAACAAAATTCTTTTTATGGATAAAAATATTGCTTTAGCTAGATGA", "aa": "MKKINVVYNPAFNQISSKLNQTQLLKNASEELDIELKFFTSFDINTTKAKANLPFISNKILFMDKNIALAR", "aa_hexdigest": "e05f80ee5e45e436e13d84dfe5dec6d4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8136.400799999994, "isoelectric_point": 9.631657218933103 }, "id": "KMADJIBOEG_66", "locus": "GCA000292445_00024" }, { "type": "cds", "sequence": "contig_1", "start": 14500, "stop": 15135, "strand": "-", "frame": 1, "gene": "folD", "product": "Bifunctional protein FolD", "db_xrefs": [ "COG:COG0190", "COG:H", "GO:0000105", "GO:0004477", "GO:0004488", "GO:0005829", "GO:0006164", "GO:0009086", "GO:0035999", "SO:0001217", "UniRef:UniRef50_P47259", "UniRef:UniRef90_P47259" ], "nt": "GTGATTATTCAAGCTAATGATGATGATTCATCAGACAGTTTTATTAAACAAAAACTTATTGCTTGTAACACTGTAGGAGCAAAAAGTGAATTAATTAAACTATCTAATCAAATAACTCAAGCAGAGTTAATAGAAAAAATAATTAGTTTAAATCATGATGTAAATGTTACTGGTATCATTTTGCAATTACCAGTTTATCCACACTTAGATAAAAACTCACTACTAGAAGCAATTAATCCTTTAAAAGATGTTGATGGTTTAACAACTAATCATTTGGCTGAAATTAAACCTTGTATAGTTGAAGCTATAATAACACTAAAAGAACTATTTAACCTTGAATTTAATAATCAAAAAATTGTTGTGGTAGGTTTGGGAATAACTGGTGGCAAACCTATTTATGAATTTTTAAAAACTAGTGGTTATAAAGTTCAAGCATGCGATAAAGATACTCCAAATACATTTGAATTGATTAAAAGTGCTGATATAGTTTTTACTGCTATTGGAAAATCTCATTTTTTTCAAGCTAAAAACTTTAAAAAAGGAGTTATTTTATTTGATATAGGTGTTTCAAGAAACAAGCAAAATAAACTTTGTGGTGATATAAATCCTGAAGGCATTGAAAAAAAAGCTAGATGA", "aa": "MIIQANDDDSSDSFIKQKLIACNTVGAKSELIKLSNQITQAELIEKIISLNHDVNVTGIILQLPVYPHLDKNSLLEAINPLKDVDGLTTNHLAEIKPCIVEAIITLKELFNLEFNNQKIVVVGLGITGGKPIYEFLKTSGYKVQACDKDTPNTFELIKSADIVFTAIGKSHFFQAKNFKKGVILFDIGVSRNKQNKLCGDINPEGIEKKAR", "aa_hexdigest": "422cef830fe9d044569da2be86c1fe31", "start_type": "GTG", "rbs_motif": null, "genes": [ "folD" ], "truncated": "both", "pseudogene": { "start": 14392, "stop": 15213, "observations": { "mutation": [ 14439, 14499, 14502, 15153 ], "stop": [ 14439, 14499, 14502, 15153 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47259", "query_cov": 1.0, "subject_cov": 0.7728937728937729, "identity": 0.995, "score": 412.0, "evalue": 9.95e-145, "gene_start": 1, "gene_end": 211, "reference_start": 27, "reference_end": 237, "reference_sequence": "MSFDGKLKAQSILETYKNFDWSKCKLVIIQANDDDSSDSFIKQKLIACNTVGAKSELIKLSNQITQAELIEKIISLNHDVNVTGIILQLPVYPHLDKNSLLEAINPLKDVDGLTTNHLAEIKPCIVEAIITLKELFNLEFNNQKIVVVGLGITGGKPIYEFLKTSGYKVQACDKDTPNTFELIKSADIVFTAIGKSHFFQAKNFKKGVILFDIGVSRNKQNKLCGDINPEGIEKKARWWTKTPGGVGPFTVLAIIKNLWILHEKNKRCLQSSI" }, "paralog": false, "identity": 0.978021978021978, "score": 520.0, "evalue": 1.03e-189, "description": "Internal stop codon at 14439, 14499, 14502, 15153. Nonsense mutation around 14439, 14499, 14502, 15153.", "psc": { "uniref90_id": "UniRef90_P47259", "gene": "folD", "product": "Bifunctional protein FolD", "uniref50_id": "UniRef50_P47259", "cog_id": "COG0190", "cog_category": "H", "go_ids": [ "GO:0005829", "GO:0004477", "GO:0004488", "GO:0000105", "GO:0009086", "GO:0006164", "GO:0035999" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47259", "UniRef:UniRef50_P47259", "COG:COG0190", "COG:H", "GO:0005829", "GO:0004477", "GO:0004488", "GO:0000105", "GO:0009086", "GO:0006164", "GO:0035999" ] }, "pscc": { "uniref50_id": "UniRef50_P47259", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47259" ], "product": "Bifunctional protein FolD" } }, "id": "KMADJIBOEG_67", "locus": "GCA000292445_00025" }, { "type": "tRNA", "sequence": "contig_1", "start": 15291, "stop": 15366, "strand": "-", "gene": "trnA", "product": "tRNA-Ala(tgc)", "amino_acid": "Ala", "anti_codon": "tgc", "score": 68.8, "nt": "GGGGATGTAGCTCAACTGATAGAGCACCTGATTTGCACTCAGGAGGTTGAGGGTTTGAGACCCTTCATCTCCACCA", "db_xrefs": [ "SO:0000254" ], "anti_codon_pos": [ 15331, 15333 ], "id": "KMADJIBOEG_36", "locus": "GCA000292445_00026" }, { "type": "tRNA", "sequence": "contig_1", "start": 15372, "stop": 15448, "strand": "-", "gene": "trnI", "product": "tRNA-Ile(gat)", "amino_acid": "Ile", "anti_codon": "gat", "score": 84.3, "nt": "GGAAGTATAGCTCAGTTGGTTAGAGCACACCCCTGATAAGGGTGAGGTCGATGGTTCAAGTCCATTTACTTCCACCA", "db_xrefs": [ "SO:0000263" ], "anti_codon_pos": [ 15412, 15414 ], "id": "KMADJIBOEG_35", "locus": "GCA000292445_00027" }, { "type": "cds", "sequence": "contig_1", "start": 15552, "stop": 16307, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B41887", "UniRef:UniRef90_UPI0001B41887" ], "nt": "ATGGGGCTTGTTTTAAAAGAATTTAACAATAAAATAAGAACAGCATTAATTCTCGCTCCTTTTTTCACTTTCGCTCAGATAGTGATTGATTTAATCATTCCTTCTTTTTTAGCAAGTGCTATTTCAGTTGTGTTTTCAATTGATAAATTAAAACAGGATGAGTCAGGCGGAAAGACAATTTCAGTTGATTTTATTGGTGGGGCTAATATTAATTTTGCTAATGTAAGAGAAGCGCAAATAGTTTTAGCAACCACAGTTATTTTACTTGCATTATGTGGACTTTTTTTTGGTCTAATATCTATTTATTGTGCAAGTTATGTTTCAGCAAACACTTCTTATCTATTAAGAAAAAAGATCTTTGCAAAATTAATGCGAATCACAACCCCATCACATGACCATTATGGTTCTTCTACTTTATTAGTTAGATTAACAAATGATGTTTATTTGATGGAGGTAATTGCTTTTGATTTTTTAAGGCTAATTATTCGTGCTCCGCTTTTGTTTATAGGTGGATTAGTGTTTGCAGTAACTACAAATCAAGATATGTCAATATCTTTACTAATTACTTTTCCTTTGATTCTTTTGGTAATTGGTATTCTAAACCGTAAATCTATTCCTTTATTTAAAGAAAATCAAAAATCAGTTGATAAGATAAATGAAAGAGTAGAAGAAGATGTTTCAGGTTATAAAGTAATTCAATCTTTTAATCTTCATTCCTTTACAAATAACAAATTTAAGATTGCAAATGAAGGATGA", "aa": "MGLVLKEFNNKIRTALILAPFFTFAQIVIDLIIPSFLASAISVVFSIDKLKQDESGGKTISVDFIGGANINFANVREAQIVLATTVILLALCGLFFGLISIYCASYVSANTSYLLRKKIFAKLMRITTPSHDHYGSSTLLVRLTNDVYLMEVIAFDFLRLIIRAPLLFIGGLVFAVTTNQDMSISLLITFPLILLVIGILNRKSIPLFKENQKSVDKINERVEEDVSGYKVIQSFNLHSFTNNKFKIANEG", "aa_hexdigest": "e3d948e9cf5764e3c0f6c60d551a7700", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 15552, "stop": 16610, "observations": { "mutation": [ 16305 ], "stop": [ 16305 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001B41887", "query_cov": 1.0, "subject_cov": 0.6519480519480519, "identity": 0.996, "score": 461.0, "evalue": 1.22e-161, "gene_start": 1, "gene_end": 251, "reference_start": 1, "reference_end": 251, "reference_sequence": "MGLVLKEFNNKIRTALILAPFFTFAQIVIDLIIPSFLASAISVVFSIDKLKQDESGGKTISVDFIGGANINFANVREAQIVLATTVILLALCGLFFGLISIYCASYVSANTSFLLRKKIFAKLMRITTPSHDHYGSSTLLVRLTNDVYLMEVIAFDFLRLIIRAPLLFIGGLVFAVTTNQDMSISLLITFPLILLVIGILNRKSIPLFKENQKSVDKINERVEEDVSGYKVIQSFNLHSFTNNKFKIANEGWKKNSTSSLFINSLNIPFTFFLSSLTIIIALLLVFQLDSSVSVDPLPQDAAIRPNIFAFFQYNFYIVLGFILTSLTMVNFNRSRVALGRIKDILSQPEIKTITNKDQKELLPTLEFRNISFGLGNKNNNNFFTK" }, "paralog": false, "identity": 0.9914772727272727, "score": 663.0, "evalue": 1.44e-243, "description": "Internal stop codon at 16305. Nonsense mutation around 16305.", "psc": { "uniref90_id": "UniRef90_UPI0001B41887", "product": "ABC transporter permease", "uniref50_id": "UniRef50_UPI0001B41887", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001B41887", "UniRef:UniRef50_UPI0001B41887" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001B41887", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B41887" ], "product": "ABC transporter permease" } }, "id": "KMADJIBOEG_68", "locus": "GCA000292445_00028" }, { "type": "cds", "sequence": "contig_1", "start": 16533, "stop": 16964, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGGTGAACTTTAATCGTTCTAGAGTTGCACTAGGCAGAATTAAAGATATTTTATCTCAGCCTGAAATAAAAACTATTACAAATAAAGATCAAAAAGAATTATTACCTACCTTGGAGTTTAGAAATATCTCTTTTGGTCTTGGCAATAAGAACAATAACAATTTTTTACAAAATCTTAGTTTTAAATTTGAAGCATATAAAACATATGGTATTGTTGGGCCTACAGGCTCTGGAAAATCTTTAATTGCTAACATTATCGGTGGTTTATATGAGCCTAATGAGGGTGAAATTCTCATTGGTGGGGAGAAAATTCAATCTATTGACAGTTTATATCTATCAGAAATGATAGGAATTGTTTTCCAACAAAACATCCTTTTTAAAGGAACAATTTCTTCTAATATAAAAATTGGTATTGAAACTAGAAGTGATTGA", "aa": "MVNFNRSRVALGRIKDILSQPEIKTITNKDQKELLPTLEFRNISFGLGNKNNNNFLQNLSFKFEAYKTYGIVGPTGSGKSLIANIIGGLYEPNEGEILIGGEKIQSIDSLYLSEMIGIVFQQNILFKGTISSNIKIGIETRSD", "aa_hexdigest": "67a4154d88c79d2e2ace51672c06c0d0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 76, "aa_cov": 0.5244755244755245, "hmm_cov": 1.0, "evalue": 4.395809451939772e-17, "score": 58.12670135498047, "start": 56, "stop": 130 } ], "seq_stats": { "molecular_weight": 15873.016799999996, "isoelectric_point": 8.858745765686034 }, "id": "KMADJIBOEG_69", "locus": "GCA000292445_00029" }, { "type": "cds", "sequence": "contig_1", "start": 16998, "stop": 17423, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DF8197" ], "nt": "ATGAAAAACGCAGCTAAAATAGCTTGCGCTGACACCTTTATTGAAAAGTTTTCTGATAGTTATGATCACAATGTTGAACAGTTAGGTAAAAACTTATCTGGTGGACAAAAACAAAGAGTTGCTATTGCAAGAACTTTAATTACAAAACCAAGAATTTTAGTTTTTGATGATAGTATGAGTGCTCTTGATGCTCTAACTGAAAAAAAAGTAAGAGAAAATATTGAAAATGATTTAAAGCTAACTACCAAAATCATTATTAGTCAAAACATTAATTCAATTAAACACGCAGATAAAATTTTGGTAATTGATAATGGCAGAATTGTTGGTTTTGATAGTGATCAAAAACTAATGAAAAATTGTTCACTTTATCAAAAGATGAAAGAATCTCAAAAAGATTTGGGAGGTGATTTTGATGCGGTTAACTAG", "aa": "MKNAAKIACADTFIEKFSDSYDHNVEQLGKNLSGGQKQRVAIARTLITKPRILVFDDSMSALDALTEKKVRENIENDLKLTTKIIISQNINSIKHADKILVIDNGRIVGFDSDQKLMKNCSLYQKMKESQKDLGGDFDAVN", "aa_hexdigest": "4b00981fe19cc59148815d4e1c1e9d57", "start_type": "ATG", "rbs_motif": "ATTTA", "psc": { "uniref90_id": "UniRef90_UPI0021DF8197", "query_cov": 0.9219858156028369, "subject_cov": 0.8441558441558441, "identity": 0.546, "score": 140.0, "evalue": 3.06e-40, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI0021DF8197" }, "pscc": { "uniref50_id": "UniRef50_UPI0021DF8197", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DF8197" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "KMADJIBOEG_70", "locus": "GCA000292445_00030" }, { "type": "cds", "sequence": "contig_1", "start": 17509, "stop": 17781, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTGTATAGTTCTTGGTATTTTATATGGAATTGCTAACCCTATCTTATTAGCACAAGGTCTTGGTTTTATTTTTCCTATTACTAATAGTAATGGTCGTGCTGTTGACTCAATATATTCATTAATTTACCCAACAAATTTAAATGTATTCATTAGGCTCACAATTGTGAGCGTAACTGTTTTTATAGCTTATGCATTAATCTTTGTATTTAATGTAGCGCAAAACTATGTAGGGATTAAACTTTACCAGCAAACATGTGCTACTTTGCGTTGA", "aa": "MCIVLGILYGIANPILLAQGLGFIFPITNSNGRAVDSIYSLIYPTNLNVFIRLTIVSVTVFIAYALIFVFNVAQNYVGIKLYQQTCATLR", "aa_hexdigest": "a004c9998b828b22d5e5636d2eee90a4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9934.723599999994, "isoelectric_point": 9.137958335876466 }, "id": "KMADJIBOEG_71", "locus": "GCA000292445_00031" }, { "type": "cds", "sequence": "contig_1", "start": 17797, "stop": 18411, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGAGTATGTCAACCAGCTTTTTTGATACGCAAAATAATGGTGATCTTATGAGTAGGTTAACTAATGATATGTATAACATTGATAACCTATTCACTCAAGCTGGAGGACAAGCTATTCAAAGTTTGTTTAATATTTTAACAACCTCAGTATTAATATTTTTATTAAGTCCAGTTATTGCACCTATTTCACTTTCAATTTTAGCTACATTAATTACTTTTTCTTTTGCCTTTCTAAAGAAATCAAAAACTTCATATAGTCAAGTACAAAATAATTTGGGTGATATGTCTGGTTATATTGAAGAGGTTTTAACTAATCATAAGGTTGTTCATGTCTTGAAGTTGCAAGAGATAATGATTAAGGATTTTGATCAATACAACAAATCAATGATCAAATCAACTGTAAGAGGGAATACATATTCGATCTTTCTTTTTTCTTGGTTTGGTTTTATATCAAATATTACTTATCTGGTTTCTATATCAATTGCTACTGCTTTTAGTGTTAATTCTATTCCTTCATTTGGAATTAGTGTTATTAACTATTCATTCATGTTGTCTTACATTGCTTCTTTAAGGCAAATAACTTTAGCATTAGATAAAATCTTTACCCTTTGA", "aa": "MQSMSTSFFDTQNNGDLMSRLTNDMYNIDNLFTQAGGQAIQSLFNILTTSVLIFLLSPVIAPISLSILATLITFSFAFLKKSKTSYSQVQNNLGDMSGYIEEVLTNHKVVHVLKLQEIMIKDFDQYNKSMIKSTVRGNTYSIFLFSWFGFISNITYLVSISIATAFSVNSIPSFGISVINYSFMLSYIASLRQITLALDKIFTL", "aa_hexdigest": "bf1bf299cb1bcde9467a3018ca48f1b3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22883.263900000016, "isoelectric_point": 7.906031990051269 }, "id": "KMADJIBOEG_72", "locus": "GCA000292445_00032" }, { "type": "cds", "sequence": "contig_1", "start": 18883, "stop": 19239, "strand": "+", "frame": 1, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X7NT64" ], "nt": "ATGCGTTTACCAAAAGGATATGACACTTATATTTCCAATAAAGCAGATTATCTTTCTGTTGGTGAAAGGCAATTATTAACAATTGCCAGAGCAGTAATCCGTAATGCTCCAGTTTTGCTCTTAGATGAAGCAACTAGTTCAGTTGATGTCCATTCAGAAAAATTAATTCAAGAATCAATAGGAAGGTTAATGAAAAATAAAACTTCTTTTATAATTTCTCATCGTCTTTCAATTATTCGTGATGCAACATTAATAATGGTTATTAATGATGGTAAAGTACTTGAAATGGGTAATCATGATCAGCTGATGAAACAAAATGGATTTTATGCACGTTTAAAACAATCTTCGGTTCGTTAA", "aa": "MRLPKGYDTYISNKADYLSVGERQLLTIARAVIRNAPVLLLDEATSSVDVHSEKLIQESIGRLMKNKTSFIISHRLSIIRDATLIMVINDGKVLEMGNHDQLMKQNGFYARLKQSSVR", "aa_hexdigest": "5e64f46355b735f8f6a4435ac85fd7d0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00131ED865", "query_cov": 0.9576271186440678, "subject_cov": 0.9338842975206612, "identity": 0.5660000000000001, "score": 136.0, "evalue": 2.19e-39, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_A0A7X7NT64" }, "pscc": { "uniref50_id": "UniRef50_A0A7X7NT64", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X7NT64" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "KMADJIBOEG_73", "locus": "GCA000292445_00033" }, { "type": "cds", "sequence": "contig_1", "start": 19249, "stop": 19620, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04434.22" ], "nt": "ATGACTGTTGCTGAAATTAAAAAACTTGCATTAAATAATCAGGTATTTAATGAAGCAAAAGCGCTTTTAGAAAAAGGTAATGTTATTTTTCCAAAAAAATTCTTAAAGCGAAAGAAGATCATTATTGAAGTATTAGATGGTAAGGTTTTTAAGGTTCAAATTAATTTAAAAACCGCTGCTGCACACTTGGATTGTAGTTGCTCTAATGATAAGCAAAATTGCGTTCATATAATTGCAGCGCTTTTGAAATATAATGATCTAAAAAATCAAGATAACAAAGAATTTGACCTTAATAAAGCCGATAAATTAGAGTGCAAAGAAGTTGAAATTCTAATTGAAAATGTTAGCTTAGCAATTGTTAATGGCAGCTGA", "aa": "MTVAEIKKLALNNQVFNEAKALLEKGNVIFPKKFLKRKKIIIEVLDGKVFKVQINLKTAAAHLDCSCSNDKQNCVHIIAALLKYNDLKNQDNKEFDLNKADKLECKEVEILIENVSLAIVNGS", "aa_hexdigest": "91ce7bc8403e9eef42379ea6dc1b9554", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04434.22", "name": "SWIM", "length": 39, "aa_cov": 0.2926829268292683, "hmm_cov": 0.9743589743589743, "evalue": 1.2300915548555542e-08, "score": 29.489242553710938, "start": 50, "stop": 85 } ], "seq_stats": { "molecular_weight": 13824.102099999984, "isoelectric_point": 8.85700511932373 }, "id": "KMADJIBOEG_74", "locus": "GCA000292445_00034" }, { "type": "cds", "sequence": "contig_1", "start": 19822, "stop": 20733, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATATGAAAATAACAGTTTGCTTTTTAATTTAGATCAATTCCTAAGCCTAGTTAAGGAAGTTAAAAAACCTTCATTATTCTTATTAAATGAGGATAAAACTGACAATATTCTTTTCTTAAGAAGTCAACATAAAATCAATGGATTAAGCCACGTTTGTGGTTTTTTAAATAATAAGGTTTTTGATTTTGTATCCTACAATGAAAAAACTAAGCAAATTGTTTTACGCTTAGCTTATCTTAATAAGTTCACTGATTTTAAATTTCCATACAACATTAACATCTATAAACTTGCTTTTGGAGAAACATTATTTTTCCATTTTTTAATCCATTTAAAAATGAATGGTTTTAAAAACATCTTTTTTCAAAGTGATGTTGCTATTGTTAAGGAAAGTGAATATCTACCTAAGATGTTTTTGACTATTGAATTTAATACACAAAAAAACAAGTTTATAACTGATGCTTTTTTTAAATATAAGAATAAAAATAGCAATACTTTAACAACTGTTTACCCCCATCGCTATTACTTAGCTCAAAAAACTAATACATCAAACTTCAACCGTTTACTTTTTTATGAACAAGCACTACAAAGGTTTTATGAAGAATTATTTCAAATAGATTATTTAAGAAGATTTGAAAACATTCCCATTAAAGATAAGAATCAAATTGCGCTTTTTAAAACTGTTTTTGATGATTACAAAACCATTGATTTAGCAGAATTGAAACTTACAAGTAATCTCTTAAATTACAAACAGTTACATTTTTCAATAAGTGATATTAAAGCTTTAAAAATTGAAGATAGACAACTAAAAATTGAATTTAAAGCTGGCGGGATAGATCTAAAGTTAATTAAAAGTGTTTTAAGTAACTACTATAAGGGTAATGCTATTTGTATTGGTGAAGATGGTTGA", "aa": "MQYENNSLLFNLDQFLSLVKEVKKPSLFLLNEDKTDNILFLRSQHKINGLSHVCGFLNNKVFDFVSYNEKTKQIVLRLAYLNKFTDFKFPYNINIYKLAFGETLFFHFLIHLKMNGFKNIFFQSDVAIVKESEYLPKMFLTIEFNTQKNKFITDAFFKYKNKNSNTLTTVYPHRYYLAQKTNTSNFNRLLFYEQALQRFYEELFQIDYLRRFENIPIKDKNQIALFKTVFDDYKTIDLAELKLTSNLLNYKQLHFSISDIKALKIEDRQLKIEFKAGGIDLKLIKSVLSNYYKGNAICIGEDG", "aa_hexdigest": "a42539632621ef2876fe5cf77d4c30b2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 35975.158099999986, "isoelectric_point": 9.250906944274899 }, "id": "KMADJIBOEG_75", "locus": "GCA000292445_00035" }, { "type": "cds", "sequence": "contig_1", "start": 20740, "stop": 21174, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00176.29" ], "nt": "TTGAACGATGAAAATGCTAAAGCACTAATTTCATTTTGGAGTCAAATTGACTTAAGAAATGCTACTTGTGATGCTAATAACAATTTGCTTCTTGCTAAATACCACTTGTTTGAAGTTGTAGATACTATTAGTAAATACACTGATGTAACTAATTTATTAGATGAAAAAACAGCCTTACAATTAAAAATAGCTAGTGAAAATCAATTTCATCTTTCGTTAGATAATAACCAAATTAATAACTTACGTAAATATCAAAAAGAAGGAGTTAAATGGATAAGGGCATTAGAAGATAATCAGTTTGGTGGAATTCTAGCAGATGAAATGGGGTTAGGTAAAACTGCTCAAGTGATCTTTGCAATGCTGGATAGTTATCAATCAACAAAATCACTTTTACCTAGTTTAATTATCGTTCCAGCATCCTTACTCTTAAATTGA", "aa": "MNDENAKALISFWSQIDLRNATCDANNNLLLAKYHLFEVVDTISKYTDVTNLLDEKTALQLKIASENQFHLSLDNNQINNLRKYQKEGVKWIRALEDNQFGGILADEMGLGKTAQVIFAMLDSYQSTKSLLPSLIIVPASLLLN", "aa_hexdigest": "2f1ca9220bc4611b7188c1caec9439d5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00176.29", "name": "SNF2-rel_dom", "length": 63, "aa_cov": 0.4097222222222222, "hmm_cov": 1.0, "evalue": 4.4339456295561884e-17, "score": 57.18943405151367, "start": 84, "stop": 142 } ], "seq_stats": { "molecular_weight": 16217.3373, "isoelectric_point": 5.07500591278076 }, "id": "KMADJIBOEG_76", "locus": "GCA000292445_00036" }, { "type": "cds", "sequence": "contig_1", "start": 21574, "stop": 22125, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00271.37", "PFAM:PF00176.29" ], "nt": "ATGAAAAAAACAAGTCCTTTTATTTTAAGAAGGACTAAAAATAAAGTTTTAAAAGAACTACCTAAAAAAATTATTACTGATATCTATGTTGAACTTAGTGAAGAACATCAAAAACTGTATGATAAACAAAAAACAGATGGTTTGAAGGAGATTAAAGAAAGTGATGCTAAAAATGCTTTAAATATCCTTAGTTTAATCTTGAAATTAAGGCATATTTGTAGCTTAGTTAAAGATAATGATGTTAATGATTTTGAAGATAATTCCAAAGCTAATACAGCTTTAAACATTATTTATGAAGCACTTGAAAATAAACGCAAAGTTATTTTGTTTACTCAATTTTTAGATGTAATTGATTGTTTTAAGCAAACTTTAAAAAATCAAAAGATTGATCACCTGGTATTTGATGGTAGAAAAACTGTGAAGAATAGAAACACTATTATCCAGAAGTTCAATAGTGCTAAAGAACCTTCTGTGATGTTAGCTTCTTTAAAAGCTGGTGGAGTTGGTATTAACTTAACTGCTGCTGAAGTTGTTATCCATTTTGATGTGTGA", "aa": "MKKTSPFILRRTKNKVLKELPKKIITDIYVELSEEHQKLYDKQKTDGLKEIKESDAKNALNILSLILKLRHICSLVKDNDVNDFEDNSKANTALNIIYEALENKRKVILFTQFLDVIDCFKQTLKNQKIDHLVFDGRKTVKNRNTIIQKFNSAKEPSVMLASLKAGGVGINLTAAEVVIHFDV", "aa_hexdigest": "b0833bc074151774d888539aa310e65e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00271.37", "name": "Helicase_C", "length": 91, "aa_cov": 0.4808743169398907, "hmm_cov": 0.967032967032967, "evalue": 1.2118362323486812e-15, "score": 52.88323974609375, "start": 95, "stop": 182 }, { "id": "PF00176.29", "name": "SNF2-rel_dom", "length": 74, "aa_cov": 0.3879781420765027, "hmm_cov": 1.0, "evalue": 2.5707693532136407e-11, "score": 38.27383804321289, "start": 4, "stop": 74 } ], "seq_stats": { "molecular_weight": 20925.1637, "isoelectric_point": 9.377329444885255 }, "id": "KMADJIBOEG_77", "locus": "GCA000292445_00037" }, { "type": "cds", "sequence": "contig_1", "start": 22385, "stop": 23554, "strand": "+", "frame": 2, "gene": "dnaJ", "product": "molecular chaperone DnaJ", "db_xrefs": [ "COG:COG0484", "COG:O", "EC:1.8.4.-", "GO:0005515", "GO:0005524", "GO:0005737", "GO:0006260", "GO:0006457", "GO:0008270", "GO:0009408", "GO:0031072", "GO:0042026", "GO:0051082", "GO:0051085", "RefSeq:WP_014894099.1", "SO:0001217", "UniParc:UPI00027B3189", "UniRef:UniRef100_UPI00027B3189", "UniRef:UniRef50_P78004", "UniRef:UniRef90_P47265" ], "nt": "ATGGCTGCTGGTAAAAGGGATTATTATGAAGTTCTAGGGATATCTAAAAACGCTAGTTCTCAAGACATAAAAAGAGCTTTTAGAAAGCTTGCAATGCAATATCACCCCGATCGTCATAAAGCAGAAAATGAAACTACTCAAAAACAAAATGAGGAAAAGTTTAAAGAGGTTAATGAAGCATACGAAGTTCTAAGTGATGAAGAAAAACGTAAGCTTTATGACCAGTTTGGTCATGAAGGGTTAAATGCTTCTGGTTTTCATGAAGCAGGGTTTAATCCTTTTGACATCTTTAATAGTGTTTTTGGTGAGGGATTTTCCTTTGGAATGGATGGTGATTCACCATTTGATTTCATTTTTAATCGTTCTAAAAAACGTCAACAACAAATTGTTGTTCCCTATAACCTTGATATTGCTTTAGTAATTGAAATTAACTTTTTTGAAATGACTAATGGTTGCAACAAAACCATCGAATATGAAAGAAAAGTTTCATGTCATAGTTGTAATGGTTTTGGCGCTGAAGGCGGGGAAAGTGGATTGGATCTTTGTAAGGATTGTAATGGCAATGGTTTTGTTATTAAAAACCAACGTTCTATCTTTGGAACCATTCAATCCCAAGTCTTGTGTTCAACTTGCAATGGACAAGGAAAACAAATTAAAGTTAAGTGCAAAACTTGTCGTTCTAACAAATACACTGTTACCAATCAAATTAAAGAGATTAATATTCCAGCAGGAATGTATAGTGGTGAAGCTTTAGTTGATGAAAGTGGTGGTAATGAATTTAAAGGTCACTATGGAAAATTAATCATTCAAGTGAATGTATTGGCAAGTAAGATTTTCAAACGTAGTGATAATAATGTTATTGCCAATGTTTTAGTAGATCCAATGGTTGCTATAGTTGGTGGGGTAATTGAACTACCTACTCTTGAAGGGATTAAAGAATTTAATATTAGACCAGGCACTAAGAGTGGCGAACAAATTGTTATTCCTAACGGTGGGATTAAATTCTCAAAGAGTTTTAAAAGAAAAGCTGGGGACTTAATCATTATTATTAGTTATGCACGTCCTTGTGAATACACTAACTTAGAATTGAAAAAATTACGTGAGTTTATCAAACCTAATCAAGAGGTTAGACAATACTTAAATACTTTAAAAAATGAATACAAAACTTAA", "aa": "MAAGKRDYYEVLGISKNASSQDIKRAFRKLAMQYHPDRHKAENETTQKQNEEKFKEVNEAYEVLSDEEKRKLYDQFGHEGLNASGFHEAGFNPFDIFNSVFGEGFSFGMDGDSPFDFIFNRSKKRQQQIVVPYNLDIALVIEINFFEMTNGCNKTIEYERKVSCHSCNGFGAEGGESGLDLCKDCNGNGFVIKNQRSIFGTIQSQVLCSTCNGQGKQIKVKCKTCRSNKYTVTNQIKEINIPAGMYSGEALVDESGGNEFKGHYGKLIIQVNVLASKIFKRSDNNVIANVLVDPMVAIVGGVIELPTLEGIKEFNIRPGTKSGEQIVIPNGGIKFSKSFKRKAGDLIIIISYARPCEYTNLELKKLREFIKPNQEVRQYLNTLKNEYKT", "aa_hexdigest": "3eec74deae4441d3aa5c8f9b2719470b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3189", "ncbi_nrp_id": "WP_014894099.1", "uniref100_id": "UniRef100_UPI00027B3189", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3189", "RefSeq:WP_014894099.1", "UniRef:UniRef100_UPI00027B3189" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3189", "uniref90_id": "UniRef90_P47265" }, "psc": { "uniref90_id": "UniRef90_P47265", "gene": "dnaJ", "product": "molecular chaperone DnaJ", "ec_ids": [ "1.8.4.-" ], "uniref50_id": "UniRef50_P78004", "cog_id": "COG0484", "cog_category": "O", "go_ids": [ "GO:0005515", "GO:0005524", "GO:0005737", "GO:0006260", "GO:0006457", "GO:0008270", "GO:0009408", "GO:0031072", "GO:0042026", "GO:0051082", "GO:0051085" ] }, "pscc": { "uniref50_id": "UniRef50_P78004", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78004" ], "product": "Chaperone protein DnaJ" }, "genes": [ "dnaJ" ], "id": "KMADJIBOEG_78", "locus": "GCA000292445_00038" }, { "type": "cds", "sequence": "contig_1", "start": 23634, "stop": 23834, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATATCCATGGCGGACCTGGTTCTGGTACTGATGAAGGATGTTTAAAGTATTTTGATCTTGAAACAACTTGGATTATTTTATTAGATCAAAGAGGTTGTGGTAAGAGCAAGACTAATGATATCTTTTATGAAAATAACACTGATAAATTAGTTAGTGATTTTGAAATTTTACGTCAAAAATTAAACATTAAAAGCTGA", "aa": "MYIHGGPGSGTDEGCLKYFDLETTWIILLDQRGCGKSKTNDIFYENNTDKLVSDFEILRQKLNIKS", "aa_hexdigest": "f2ac95f2749caeac5efb7135488bbbb7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7531.424499999998, "isoelectric_point": 5.14542942047119 }, "id": "KMADJIBOEG_79", "locus": "GCA000292445_00039" }, { "type": "cds", "sequence": "contig_1", "start": 23964, "stop": 24131, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGATTAGGAAAAATGTTTTATCCTTATGAACACCAACGCTTTATGGATAGTATTCCTAAAGCTTATCAGAACAGTTATGAACAAATTGTTAACTACTGTTATGATCAATTTCAAAATGGTGATGAATCAACCAAAGAAAAACTTGCTAAAGCTTGGGTGGATTGA", "aa": "MGLGKMFYPYEHQRFMDSIPKAYQNSYEQIVNYCYDQFQNGDESTKEKLAKAWVD", "aa_hexdigest": "4fa629ae8e0979194b861e4c5b6ce087", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6607.3088000000025, "isoelectric_point": 5.156797218322752 }, "id": "KMADJIBOEG_80", "locus": "GCA000292445_00040" }, { "type": "cds", "sequence": "contig_1", "start": 24210, "stop": 24464, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATGTCACTATGCAGTTAATAAAAGTTTTTTGGATGAAAACTTCATTCTAGATAACATTAGTGTTCTTAAAAATAAAAGTATTTATTTGGCTCATGGTAGATTTGATTTGATCTGTCCTTTATATCAACCATTAGCATTAAAACAAGCATTCCCTGAATTACAACTTTATGTAACCAATAATGCTGGTCATAGTGGTAGTGATGCTAATAATTTAGCAACTATAAAACACCTTTTAAAAACTTACCTTTAA", "aa": "MECHYAVNKSFLDENFILDNISVLKNKSIYLAHGRFDLICPLYQPLALKQAFPELQLYVTNNAGHSGSDANNLATIKHLLKTYL", "aa_hexdigest": "b972969db37f342178fedff9182e500c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9524.865399999991, "isoelectric_point": 6.8863508224487315 }, "id": "KMADJIBOEG_81", "locus": "GCA000292445_00041" }, { "type": "cds", "sequence": "contig_1", "start": 24464, "stop": 24706, "strand": "+", "frame": 2, "gene": null, "product": "Methionyl/Leucyl tRNA synthetase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A093YS71" ], "nt": "ATGAAGCGTTGTTATATTACAACCCCTATCTACTACGCATCAGGTAAGCCCCATATAGGTCATGCTTTTACCACTATTTTGGCGGATGTAATTAAGCGTTTTAAAATCCAAAACGGATATGAGGCTTTTTTGCTTGTTGGCAGTGATGAACATGGCAATAAAATAGAAAGTAAAGCTAAAAGCTTAAATTTAGATCCTAAAACATTTGTTGATATTAACGCTCAAGCTTTTAAGTTAATGTGA", "aa": "MKRCYITTPIYYASGKPHIGHAFTTILADVIKRFKIQNGYEAFLLVGSDEHGNKIESKAKSLNLDPKTFVDINAQAFKLM", "aa_hexdigest": "648efa85a2d31d25aea590fb5cab23b8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A093YS71", "query_cov": 0.95, "subject_cov": 0.9743589743589743, "identity": 0.513, "score": 82.0, "evalue": 5.85e-19, "valid": false, "product": "Methionyl/Leucyl tRNA synthetase domain-containing protein", "uniref50_id": "UniRef50_A0A093YS71" }, "pscc": { "uniref50_id": "UniRef50_A0A093YS71", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A093YS71" ], "product": "Methionyl/Leucyl tRNA synthetase domain-containing protein" }, "genes": [], "id": "KMADJIBOEG_82", "locus": "GCA000292445_00042" }, { "type": "cds", "sequence": "contig_1", "start": 24974, "stop": 25090, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAGGCAAAAATCAACTTAACATTGTTCCTAAATCACGTGCTTCTGAATTAAAAAATAACTTTATAAACAATGGTTTAAACGATTTAGCATTAACAAGAAAAAATGTTACTTGA", "aa": "MIGKNQLNIVPKSRASELKNNFINNGLNDLALTRKNVT", "aa_hexdigest": "1b4e1135fda699dc66bbaaf0e280ba4a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4239.855900000001, "isoelectric_point": 10.446408653259276 }, "id": "KMADJIBOEG_83", "locus": "GCA000292445_00043" }, { "type": "cds", "sequence": "contig_1", "start": 25367, "stop": 25762, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAAATCTTTAAACAACGTTATCTCTCCAGAACAATTAATTGATCAATTTGGTGTTGATGGTACAAGATATTGTTTATTAAAAGAGATGCGTTTAGATAAAGATAATCGTTGTAGTATTAGCATCTTAAAAGAGATTTATAATGCTGATCTTGCCAATAGTTTTGGAAACCATGTTTCACGTACTTTTGGCATGATTAAAAAGTATCTAAACGGCAAATTAGAATACCAAATTATTACTGATAATGCACTTCAAAAAATAATGATTTTAATAGATGAATCAATCGTTCAATTTGATCATTACTTTAACAGTTATGAATTTTATAGAGCGATTAATCTACTTTTAAAAATTGTTTTTGAATTAAGTAAATTAATTGATGATTTCAAACCATGA", "aa": "MSKSLNNVISPEQLIDQFGVDGTRYCLLKEMRLDKDNRCSISILKEIYNADLANSFGNHVSRTFGMIKKYLNGKLEYQIITDNALQKIMILIDESIVQFDHYFNSYEFYRAINLLLKIVFELSKLIDDFKP", "aa_hexdigest": "81671542dc01c1aaf7feb68c710d5a1f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15311.55809999999, "isoelectric_point": 6.095095252990721 }, "id": "KMADJIBOEG_84", "locus": "GCA000292445_00044" }, { "type": "cds", "sequence": "contig_1", "start": 25766, "stop": 26002, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAAAAATCAGGAATTCTCACTTTTAAAACAACTACTTTTTACTTGTGTTAGGTGTGTGCAGGTATGCTATGTGTTGTTAACACCTATCTTAGTAAATACTGCTTCAAAAGTTTTTCATTTATTTAATTTCGCTGATGATGCCTGTAGAAAAGACCAATTAAGAGATGCAACTTTATTAAAAAAAATTATTATCTCTAATTCAATGGAAGTTTTATTTAAAAGAGTAGATTAA", "aa": "MFKNQEFSLLKQLLFTCVRCVQVCYVLLTPILVNTASKVFHLFNFADDACRKDQLRDATLLKKIIISNSMEVLFKRVD", "aa_hexdigest": "6f5655566eed17e86cdd8dfc17e96edd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9085.770299999996, "isoelectric_point": 9.070653343200682 }, "id": "KMADJIBOEG_85", "locus": "GCA000292445_00045" }, { "type": "cds", "sequence": "contig_1", "start": 26032, "stop": 26124, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATTAGAGTACCTCAATCTTATTTCTCAAGCTAAAGTTATTGCAGAAAAACAATTTAAAGCTAACCCTTTTTCTTTTGAAACAATTTGA", "aa": "MQLEYLNLISQAKVIAEKQFKANPFSFETI", "aa_hexdigest": "dc9fa5bbd9bd6161886b243b351fd3a1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3502.0416000000005, "isoelectric_point": 5.985566520690917 }, "id": "KMADJIBOEG_86", "locus": "GCA000292445_00046" }, { "type": "cds", "sequence": "contig_1", "start": 26257, "stop": 26469, "strand": "+", "frame": 1, "gene": "rpoE", "product": "DNA-directed RNA polymerase subunit delta", "db_xrefs": [ "COG:COG3343", "COG:K", "EC:2.7.7.6", "GO:0000428", "GO:0003899", "GO:0006351", "GO:0006355", "KEGG:K03048", "SO:0001217", "UniRef:UniRef50_P47268", "UniRef:UniRef90_P47268" ], "nt": "ATGAAGTTTGATGAATGGAACAAGATATCACAATCTATGTTTGTTACAAAGGAGATTTTTGAAGAAGGTTATGAAGATCTTTCCAATAAAAAAGTAGAACCTGAGGAAGGAGTTGGTGATTTCATTATGGGAAATGATGGTGATGACAATGAAACTGGCAGTGAAATAGTACAAGGTTTAATTAATGATTCATTCAGTGAGGAAAATCAATAG", "aa": "MKFDEWNKISQSMFVTKEIFEEGYEDLSNKKVEPEEGVGDFIMGNDGDDNETGSEIVQGLINDSFSEENQ", "aa_hexdigest": "33251ba9ccc549576565c89ea720e8dd", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpoE" ], "truncated": "5-prime", "pseudogene": { "start": 26032, "stop": 26469, "observations": { "mutation": [ 26122, 26239 ], "stop": [ 26122, 26239 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47268", "query_cov": 1.0, "subject_cov": 0.4827586206896552, "identity": 1.0, "score": 139.0, "evalue": 5.57e-41, "gene_start": 1, "gene_end": 70, "reference_start": 76, "reference_end": 145, "reference_sequence": "MQLEYLNLISQAKVIAEKQFKANPFSFETIWKEVVKHFKISKQDEPSLIGRFYQDFLEDPNFVYLGDRKWKLRDFMKFDEWNKISQSMFVTKEIFEEGYEDLSNKKVEPEEGVGDFIMGNDGDDNETGSEIVQGLINDSFSEENQ" }, "paralog": false, "identity": 0.9862068965517241, "score": 287.0, "evalue": 6.01e-102, "description": "Internal stop codon at 26122, 26239. Nonsense mutation around 26122, 26239.", "psc": { "uniref90_id": "UniRef90_P47268", "gene": "rpoE", "product": "DNA-directed RNA polymerase subunit delta", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P47268", "cog_id": "COG3343", "cog_category": "K", "kegg_orthology_id": "K03048", "go_ids": [ "GO:0000428", "GO:0003899", "GO:0006351", "GO:0006355" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47268", "EC:2.7.7.6", "UniRef:UniRef50_P47268", "COG:COG3343", "COG:K", "KEGG:K03048", "GO:0000428", "GO:0003899", "GO:0006351", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_P47268", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47268" ], "product": "Probable DNA-directed RNA polymerase subunit delta" } }, "id": "KMADJIBOEG_87", "locus": "GCA000292445_00047" }, { "type": "cds", "sequence": "contig_1", "start": 26496, "stop": 27026, "strand": "+", "frame": 3, "gene": null, "product": "Fructose-bisphosphate aldolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P7V3" ], "nt": "ATGCTTCAAAAAGCAAAGCTAGGTAAATATGCAATCCCTCACATTAACATCAATAACTATGAATGGGCCAAAGCTGTTTTAACAGCAGCAAATCAAGCTAATAGCCCAATTATTGTTTCAGTATCTGAAGGTGCTTTAAAGTACATGTCTGGTTATAGTGTTGTTATCCCGCTTGTTAAGGGTTTAATGGAATCACTAAGTATTAAAGTACCAGTGACATTACATTTAGATCATGGTAGTTATGATGCATGTGTCCAAGCATTACAGGCTGGATTTAGTTCAGTAATGTTTGATGGTTCACATTTACCATTTGAAGAAAATTTCAATAAATCTAAAAAGTTAATAGAGATAGCACAAAAAACAAATGCTTCTGTTGAACTTGAAGTTGGTACTATTGGTGGAGAAGAAGATGGTGTTATAGGACAAGGTGAGTTAGCTAATGTTGATGAATGTAAACAAATCGCTAGTTTAAAACCAGATGCTTTAGCAGCAGGAATTGGTAATATCCATGGTATCTATCCTAAGAATTGA", "aa": "MLQKAKLGKYAIPHININNYEWAKAVLTAANQANSPIIVSVSEGALKYMSGYSVVIPLVKGLMESLSIKVPVTLHLDHGSYDACVQALQAGFSSVMFDGSHLPFEENFNKSKKLIEIAQKTNASVELEVGTIGGEEDGVIGQGELANVDECKQIASLKPDALAAGIGNIHGIYPKN", "aa_hexdigest": "413df7c0d3e71f17a7f0a3da5bb14d05", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FEF401E", "query_cov": 0.9034090909090909, "subject_cov": 0.9034090909090909, "identity": 0.579, "score": 189.0, "evalue": 1.69e-58, "valid": false, "product": "class II fructose-bisphosphate aldolase", "ec_ids": [ "4.1.2.13" ], "uniref50_id": "UniRef50_A0A3B0P7V3", "kegg_orthology_id": "K01624" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P7V3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P7V3" ], "product": "Fructose-bisphosphate aldolase" }, "genes": [], "id": "KMADJIBOEG_88", "locus": "GCA000292445_00048" }, { "type": "cds", "sequence": "contig_1", "start": 27045, "stop": 27341, "strand": "+", "frame": 3, "gene": null, "product": "fructose-bisphosphate aldolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A097ST37" ], "nt": "TTGATTGAAACAATATCAAAAATTACTAACTTGCCCTTAGTTTTACATGGTGGCTCTGGAATCTTAGAAAATGATGTTAAAAAAGCAATTAGTTTAGGGATTTGCAAACTAAATATTAATACTGAGTGTCAATTAGCATTTGCACATGAAATTAGAAAATACATTGAATCAAATAAAGACTTGGATCTTAACAAAAAAGGTTATGATCCTAGAAAACTTTTAAAAGAACCTACTCAAGCAATTGTTGATACTTGCTTGGAAAAGATTGATTTGTGTGGTTCTAGAAATAAAGCATAG", "aa": "MIETISKITNLPLVLHGGSGILENDVKKAISLGICKLNINTECQLAFAHEIRKYIESNKDLDLNKKGYDPRKLLKEPTQAIVDTCLEKIDLCGSRNKA", "aa_hexdigest": "9cdc981a63c4f3b529d80113279022e0", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A097ST37", "query_cov": 0.9795918367346939, "subject_cov": 0.9696969696969697, "identity": 0.635, "score": 124.0, "evalue": 3.83e-35, "valid": false, "product": "fructose-bisphosphate aldolase", "ec_ids": [ "4.1.2.13" ], "uniref50_id": "UniRef50_A0A097ST37", "kegg_orthology_id": "K01624" }, "pscc": { "uniref50_id": "UniRef50_A0A097ST37", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A097ST37" ], "product": "fructose-bisphosphate aldolase" }, "genes": [], "id": "KMADJIBOEG_89", "locus": "GCA000292445_00049" }, { "type": "cds", "sequence": "contig_1", "start": 27342, "stop": 28229, "strand": "+", "frame": 3, "gene": "ychF", "product": "redox-regulated ATPase YchF", "db_xrefs": [ "COG:COG0012", "COG:J", "GO:0005524", "GO:0005525", "GO:0005737", "GO:0016887", "GO:0043023", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47270", "UniRef:UniRef90_P47270" ], "nt": "ATGTTAAGTGCAGGGATAGTTGGTTTACCTAATGTTGGTAAGTCAACTTTATTTAGTGCTATTACTAATTTGCAAGTTGAAATAGCAAACTATCCTTTTGCAACTATAGAACCTAATACTGGCATTGTTAATGTTAGTGATGAGAGATTAGATAAATTAGCTAGCTTAATTAATCCTGAAAAGATAGTTTATACAACCTTTCGTTTTGTTGATATAGCAGGTCTTGTTAAAGGCGCCAGTCAAGGTCAAGGATTGGGTAATCAATTCTTAGCAAACATCCGTGAAGTGGATTTAATTTGTCATGTTGTAAGATGTTTTCAAGATAAAAAAATTGTTCATGTTAACAATACAATAGATCCTGTTTTTGATTTTGAAATTATTGTTAATGAACTAATCCAAGCTGATTTTGAATTAATAACAAACAGAATCAGCAAACTTAAAAGAAAAGCTGAATCAGGTGATAAAATCGCTAAAGAAGAGTTTGTATTACTTGAAATTGTTTTAAATGGATTAAAACAAGGTCAAATGCCCATTCAGACTCTAAGTGAAAGTGAATTGAAAACAATTAAATCACTTAATCTATTAACAGCTAAACCTATTCTAATAGTAGCCAATGTATCTGAGAATGACTTATTAAACCTTGATAATAATGAAGCTTTAAAAAAATTGAATGCTTTTCTTGATCAAAAAAAGATTCCCAAGGCAATCACAGTTTGTTCTTTAATTGAAAAAGAATTAAGCGGTTTGAAATTAGAACAACGTCAATACTTTTTGGATGAACTTGGCTTAAAAAATTATTCAGGTTTAAACCAAGTAATTCAAGCTGCATATCAAACTTTAAACCTTTGGTCTTTTTTTACTTTTGGTAAAAAAGAAGTTAGAGCATGA", "aa": "MLSAGIVGLPNVGKSTLFSAITNLQVEIANYPFATIEPNTGIVNVSDERLDKLASLINPEKIVYTTFRFVDIAGLVKGASQGQGLGNQFLANIREVDLICHVVRCFQDKKIVHVNNTIDPVFDFEIIVNELIQADFELITNRISKLKRKAESGDKIAKEEFVLLEIVLNGLKQGQMPIQTLSESELKTIKSLNLLTAKPILIVANVSENDLLNLDNNEALKKLNAFLDQKKIPKAITVCSLIEKELSGLKLEQRQYFLDELGLKNYSGLNQVIQAAYQTLNLWSFFTFGKKEVRA", "aa_hexdigest": "90eaa26a2e7e2d88fa69f4313f57209c", "start_type": "ATG", "rbs_motif": "TTCTA", "psc": { "uniref90_id": "UniRef90_P47270", "query_cov": 1.0, "subject_cov": 0.8038147138964578, "identity": 0.993, "score": 562.0, "evalue": 6.96e-201, "valid": true, "gene": "ychF", "product": "redox-regulated ATPase YchF", "uniref50_id": "UniRef50_P47270", "cog_id": "COG0012", "cog_category": "J", "go_ids": [ "GO:0005524", "GO:0005525", "GO:0005737", "GO:0016887", "GO:0043023", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47270", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47270" ], "product": "Ribosome-binding ATPase YchF" }, "genes": [ "ychF" ], "id": "KMADJIBOEG_90", "locus": "GCA000292445_00050" }, { "type": "cds", "sequence": "contig_1", "start": 28338, "stop": 28445, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF06071.19" ], "nt": "ATGAAATCTTTACAAGAAGCTAAAAAACAAGGTTTGATAAGATTGGAAGGCAAAAATTACTTAATAAAAGATGGTGATGTTTGTAACTTTAAATTTAACGTCACTTAA", "aa": "MKSLQEAKKQGLIRLEGKNYLIKDGDVCNFKFNVT", "aa_hexdigest": "6ae3cf5b3cd0c8373b031a994816ed93", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF06071.19", "name": "YchF-GTPase_C", "length": 32, "aa_cov": 0.9142857142857143, "hmm_cov": 1.0, "evalue": 3.533830995318245e-11, "score": 38.27375411987305, "start": 2, "stop": 33 } ], "seq_stats": { "molecular_weight": 4029.6841000000013, "isoelectric_point": 9.360438728332518 }, "id": "KMADJIBOEG_91", "locus": "GCA000292445_00051" }, { "type": "cds", "sequence": "contig_1", "start": 28648, "stop": 28905, "strand": "+", "frame": 1, "gene": null, "product": "glycosyltransferase family 2 protein", "db_xrefs": [ "RefSeq:WP_237711681.1", "SO:0001217", "UniParc:UPI001ED93A4E", "UniRef:UniRef100_UPI001ED93A4E", "UniRef:UniRef50_UPI001ED93A4E", "UniRef:UniRef90_UPI001ED93A4E" ], "nt": "GTGCCAAGTAAGTATTTATTCACCGTTATAATTCCAACATACAATTGTTGTCAATACATAAAAAAGGCATTAGATTCATTGCTTTTACAGAATGAATATTTTTTAAAAACACAAGTACTAATAGTTAACGATGGTTCACTAGACAATACTAAGGAAGTTGTTAGTGACTATTTAATAAAGTACTCCAATATTAGTTATTTTGAAAAAACTAATGGTAATTGGGGCAGTGTTATTAACTATGTTAAAAAAATAAATTAG", "aa": "MPSKYLFTVIIPTYNCCQYIKKALDSLLLQNEYFLKTQVLIVNDGSLDNTKEVVSDYLIKYSNISYFEKTNGNWGSVINYVKKIN", "aa_hexdigest": "e2e0d7f429c28fc3d261ca1111827b73", "start_type": "GTG", "rbs_motif": "TTATA", "ups": { "uniparc_id": "UPI001ED93A4E", "ncbi_nrp_id": "WP_237711681.1", "uniref100_id": "UniRef100_UPI001ED93A4E", "db_xrefs": [ "SO:0001217", "UniParc:UPI001ED93A4E", "RefSeq:WP_237711681.1", "UniRef:UniRef100_UPI001ED93A4E" ] }, "ips": { "uniref100_id": "UniRef100_UPI001ED93A4E", "uniref90_id": "UniRef90_UPI001ED93A4E" }, "psc": { "uniref90_id": "UniRef90_UPI001ED93A4E", "product": "glycosyltransferase family 2 protein", "uniref50_id": "UniRef50_UPI001ED93A4E" }, "pscc": { "uniref50_id": "UniRef50_UPI001ED93A4E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED93A4E" ], "product": "glycosyltransferase family 2 protein" }, "genes": [], "id": "KMADJIBOEG_92", "locus": "GCA000292445_00052" }, { "type": "cds", "sequence": "contig_1", "start": 28971, "stop": 29300, "strand": "+", "frame": 3, "gene": null, "product": "glycosyltransferase family 2 protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FDFBC6D", "UniRef:UniRef90_UPI002FDFBC6D" ], "nt": "GTGGCTCGTTTCTTTGGCCATGACATGATCATTGGCGCTTTTTATTGTTATATTAATGAAAATAAAACTCGTTTTTTAAAACCTTATTTCGGTAAGACTGGTGTTATTAAAGAACATACCAAATTAAGAACCCCCCATTCCCAACCTATTGCTAAATTTTACAGTAATAAACTTTTCTATGAACTACATGATCTTAAGGAAAAGTTATTCTTCCAGGATTGTTTAATGTATCACGATGCTATTAACAGAGTTGAAAGTGTTTTTTATTTGCGAGAGCCTTTAGCAGTTTGGTTTTCTACAAGACCTGGCAATTCTACAACAACTTCTTGA", "aa": "MARFFGHDMIIGAFYCYINENKTRFLKPYFGKTGVIKEHTKLRTPHSQPIAKFYSNKLFYELHDLKEKLFFQDCLMYHDAINRVESVFYLREPLAVWFSTRPGNSTTTS", "aa_hexdigest": "aabad00bc954eef137f4088eab127fce", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 28950, "stop": 29543, "observations": { "mutation": [ 29298, 29463 ], "stop": [ 29298, 29463 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FDFBC6D", "query_cov": 1.0, "subject_cov": 0.5532994923857868, "identity": 0.991, "score": 227.0, "evalue": 2.04e-74, "gene_start": 1, "gene_end": 109, "reference_start": 8, "reference_end": 116, "reference_sequence": "MKDSFKKVARFFGHDMIIGAFYCYINENKTRFLKPYFGKTGVIKEHTKLRTPHSQPIAKFYSNKLFYELHDLKEKLFFQDCLMYHDAINRVESVFYLREPLAVWFSTRPGNSTTTSWENPNKFNAWCEILQKMNLYGAGIVIYIYTMLPGFLKQLKKKELILNLNHKPAYTWLPKPLAFIFGGLMAFKTRKYIKYPK" }, "paralog": false, "identity": 0.9847715736040609, "score": 405.0, "evalue": 4.34e-147, "description": "Internal stop codon at 29298, 29463. Nonsense mutation around 29298, 29463.", "psc": { "uniref90_id": "UniRef90_UPI002FDFBC6D", "product": "glycosyltransferase family 2 protein", "uniref50_id": "UniRef50_UPI002FDFBC6D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FDFBC6D", "UniRef:UniRef50_UPI002FDFBC6D" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FDFBC6D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FDFBC6D" ], "product": "glycosyltransferase family 2 protein" } }, "id": "KMADJIBOEG_93", "locus": "GCA000292445_00053" }, { "type": "cds", "sequence": "contig_1", "start": 29346, "stop": 29465, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTGTATGGAGCTGGGATAGTAATCTACATCTATACTATGCTACCTGGATTTCTAAAACAACTAAAGAAAAAAGAACTAATACTGAATTTGAACCATAAACCAGCTTACACTTGA", "aa": "MNLYGAGIVIYIYTMLPGFLKQLKKKELILNLNHKPAYT", "aa_hexdigest": "034fdddd1f30750c9811f7895d2205ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4511.438200000001, "isoelectric_point": 9.69941349029541 }, "id": "KMADJIBOEG_94", "locus": "GCA000292445_00054" }, { "type": "cds", "sequence": "contig_1", "start": 29547, "stop": 30119, "strand": "+", "frame": 3, "gene": "efp", "product": "elongation factor P", "db_xrefs": [ "COG:COG0231", "COG:J", "GO:0003746", "GO:0005737", "GO:0006414", "GO:0043043", "KEGG:K02356", "RefSeq:WP_014894103.1", "SO:0001217", "UniParc:UPI00027B318F", "UniRef:UniRef100_UPI00027B318F", "UniRef:UniRef50_P75085", "UniRef:UniRef90_P47272" ], "nt": "ATGGCAGAAATGATAGAAGCAAAAAATCTTCGTAATGGGCAAACCATCTTCGGTCCTAACAAAGAGATTTTATTAGTACTGGAAAATACATTTAACAAAACTGCAATGCGCCAGGGAATTGTTAAAACTAAAGTTAAGAACTTAAGAACTGGGGCTATTGTTTGGCTTGAATTTACTGGTGACAAATTAGAACAAGTAATTATTGATAAGAAAAAAATGAATTTCTTATACAAAGATGGTAATAACTTTGTTTTTATGGATCAAAAAGACTACAGTCAGATTGAGATTAATGAAAAAAAATTAGAGTGGGAAAAAAATTTCATTACTGAAGAAATTGAAGTTACTGTTATTACTTATCAAGATGAAATTCTAGGAGTTAATTTACCTGATTTAGTTCCTATTGAAGTTGAGTTTGCTGAAGATGCTATTCAGGGCAATACTGCTAACATGGCAAGAAAAAAAGCACGCCTTGTAACTGGTTATGAACTTGATGTACCCCAATTTATTAATACTGGTGATAAGATTGTAATTGCCACTATTGATGGCAATTACCGTGAAAGGTTTAACAAATAA", "aa": "MAEMIEAKNLRNGQTIFGPNKEILLVLENTFNKTAMRQGIVKTKVKNLRTGAIVWLEFTGDKLEQVIIDKKKMNFLYKDGNNFVFMDQKDYSQIEINEKKLEWEKNFITEEIEVTVITYQDEILGVNLPDLVPIEVEFAEDAIQGNTANMARKKARLVTGYELDVPQFINTGDKIVIATIDGNYRERFNK", "aa_hexdigest": "4b720a55fbb962d599ccaf1d21c12b95", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B318F", "ncbi_nrp_id": "WP_014894103.1", "uniref100_id": "UniRef100_UPI00027B318F", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B318F", "RefSeq:WP_014894103.1", "UniRef:UniRef100_UPI00027B318F" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B318F", "uniref90_id": "UniRef90_P47272" }, "psc": { "uniref90_id": "UniRef90_P47272", "gene": "efp", "product": "elongation factor P", "uniref50_id": "UniRef50_P75085", "cog_id": "COG0231", "cog_category": "J", "kegg_orthology_id": "K02356", "go_ids": [ "GO:0003746", "GO:0005737", "GO:0006414", "GO:0043043" ] }, "pscc": { "uniref50_id": "UniRef50_P75085", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75085" ], "product": "Elongation factor P" }, "genes": [ "efp" ], "id": "KMADJIBOEG_95", "locus": "GCA000292445_00055" }, { "type": "cds", "sequence": "contig_1", "start": 30088, "stop": 30255, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09185.15" ], "nt": "ATGGCAATTACCGTGAAAGGTTTAACAAATAAATTAACTAGAACCCAAAGACGAATTGCTGTAGTGGAATTTATCTTTTCTCTCCTCTTTTTTTTACCTAAAGAAGCTGAAGTTATTCAAGCAGATTTTTTAGAATATGATACTAAAGAACGACAACTAAATGAATGA", "aa": "MAITVKGLTNKLTRTQRRIAVVEFIFSLLFFLPKEAEVIQADFLEYDTKERQLNE", "aa_hexdigest": "fd22dea1bdfd44b29cbd46360442dd47", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09185.15", "name": "DUF1948", "length": 44, "aa_cov": 0.8, "hmm_cov": 1.0, "evalue": 9.061191704481707e-25, "score": 82.33567810058594, "start": 12, "stop": 55 } ], "seq_stats": { "molecular_weight": 6475.4695999999985, "isoelectric_point": 6.225256538391113 }, "id": "KMADJIBOEG_96", "locus": "GCA000292445_00056" }, { "type": "cds", "sequence": "contig_1", "start": 30325, "stop": 30543, "strand": "+", "frame": 1, "gene": "nusB", "product": "putative protein MG027", "db_xrefs": [ "COG:COG0781", "COG:K", "SO:0001217", "UniRef:UniRef50_P47273", "UniRef:UniRef90_P47273" ], "nt": "TTGAAAAATCAATTAGAAATTCAAACTAAATACAATAAAATATCAGGAAAAAAGATTGATCTTTTAACTACTGCAGTTGTTTTATGTGCACTTAGTGAACAAAAGGCACATAATACTGATAAACCACTTTTAATTAGTGAGGCATTGTTGATTATGGATCGTTATTCACAAGGTGCTGAAAAAAAACAAACTCATGCTTTATTAGATAAGCTCTTGTAA", "aa": "MKNQLEIQTKYNKISGKKIDLLTTAVVLCALSEQKAHNTDKPLLISEALLIMDRYSQGAEKKQTHALLDKLL", "aa_hexdigest": "649c420bb67839b397dcf26686b71fca", "start_type": "TTG", "rbs_motif": null, "genes": [ "nusB" ], "truncated": "5-prime", "pseudogene": { "start": 30088, "stop": 30543, "observations": { "mutation": [ 30253 ], "stop": [ 30253 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47273", "query_cov": 1.0, "subject_cov": 0.4768211920529801, "identity": 0.972, "score": 137.0, "evalue": 2.95e-40, "gene_start": 1, "gene_end": 72, "reference_start": 80, "reference_end": 151, "reference_sequence": "MAITVKGLTNKLTRTQRRIAVVEFIFSLLFFLPKEAEVIQADFLEYDTKERQLNEWQKLIVKAFSENIFSFQKKIEEQQLKNQLEIQTKYNKISGKKIDLLTTAVVLCALSEQKAHNTDKPLLISEALLIMDHYSQGAEKKQTHALLDKLL" }, 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Nonsense mutation around 30253.", "psc": { "uniref90_id": "UniRef90_P47273", "gene": "NusB", "product": "Uncharacterized protein MG027", "uniref50_id": "UniRef50_P47273", "cog_id": "COG0781", "cog_category": "K", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47273", "UniRef:UniRef50_P47273", "COG:COG0781", "COG:K" ] }, "pscc": { "uniref50_id": "UniRef50_P47273", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47273" ], "product": "Uncharacterized protein MG027" } }, "id": "KMADJIBOEG_97", "locus": "GCA000292445_00057" }, { "type": "cds", "sequence": "contig_1", "start": 30978, "stop": 31148, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAAAACTGCACATTTTTTTATTGATATAGAGATTTTGAAAGCCAAGAAAGGAGAACCCACTGATCCTAATAAAACTGATAATAGAGCAGTTGTTATTAATCTTGATGAGAATAAAAAGAATGAAAAAGAACAGTCCCCTCCTTCTGCAGAAATGACTAGTCTTTAA", "aa": "MSKTAHFFIDIEILKAKKGEPTDPNKTDNRAVVINLDENKKNEKEQSPPSAEMTSL", "aa_hexdigest": "54fd8293bb6a9a7188abedcdfb7798ba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6300.047800000002, "isoelectric_point": 5.7142740249633786 }, "id": "KMADJIBOEG_98", "locus": "GCA000292445_00058" }, { "type": "cds", "sequence": "contig_1", "start": 31138, "stop": 31731, "strand": "-", "frame": 1, "gene": "yajL", "product": "putative protein MG029", "db_xrefs": [ "COG:COG0693", "COG:V", "GO:0005737", "SO:0001217", "UniRef:UniRef50_P47275", "UniRef:UniRef90_P47275" ], "nt": "ATGCTGGGGACCGTTTATTTGGTACTAAATAAAATGAAAAAGATACTTGTTATTGTCTATCCAGAGATGAATGATGTTGAATATACTAATGTAATGGTTGTATTTAGTTTCATTAAAACAATACAAACAACATGTTATCACCATTCATTAAAAAAGATAACTGCTAGTAATGGTGTTGTTGAAGTTAACAATATTGTCAACACCATTAACTTATCAGAATTTGATGCAGTTTATATTCCCGGTGGAATAGGAGCTACAAAACACTTAGATAAAGATGAAAAACTGCTTAAAACAATTAATTATTTCAAAGTAAATAACCTTTATCTTTTTGCTATTTGTGACACACCGAATGTTCTATTTAAACATGGTATTATCACTAAAGATGAGATCTATTCTTCTTTTCCAAACCCAAATTTAGTTATGTCTGAAAACAGATCAACAGCAAAAGTAACAGTAGCAAATAAATTAATAACAGCAAGAAGTGCTGGTTGTGCTTTGGAATTTGCAACAGTTATTGTGTGCACTTTTTTAAAAGATAACACTTTAAATGAGCTTGTTCATAAAAGGTTATTTGGTAGTGAAATTAAAGACTAG", "aa": "MLGTVYLVLNKMKKILVIVYPEMNDVEYTNVMVVFSFIKTIQTTCYHHSLKKITASNGVVEVNNIVNTINLSEFDAVYIPGGIGATKHLDKDEKLLKTINYFKVNNLYLFAICDTPNVLFKHGIITKDEIYSSFPNPNLVMSENRSTAKVTVANKLITARSAGCALEFATVIVCTFLKDNTLNELVHKRLFGSEIKD", "aa_hexdigest": "6f4c7a7c877f5790514d8340318d40ec", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47275", "query_cov": 0.9441624365482234, "subject_cov": 1.0, "identity": 1.0, "score": 369.0, "evalue": 4.24e-129, "valid": true, "gene": "YajL", "product": "Uncharacterized protein MG029", "uniref50_id": "UniRef50_P47275", "cog_id": "COG0693", "cog_category": "V", "go_ids": [ "GO:0005737" ] }, "pscc": { "uniref50_id": "UniRef50_P47275", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47275" ], "product": "Uncharacterized protein MG029" }, "genes": [ "yajL" ], "id": "KMADJIBOEG_99", "locus": "GCA000292445_00059" }, { "type": "cds", "sequence": "contig_1", "start": 31700, "stop": 32320, "strand": "-", "frame": 3, "gene": "upp", "product": "uracil phosphoribosyltransferase", "db_xrefs": [ "COG:COG0035", "COG:F", "EC:2.4.2.9", "GO:0000287", "GO:0004845", "GO:0005525", "GO:0005737", "GO:0006223", "GO:0044206", "RefSeq:WP_009885912.1", "SO:0001217", "UniParc:UPI0000137D2A", "UniRef:UniRef100_P47276", "UniRef:UniRef50_P47276", "UniRef:UniRef90_P47276" ], "nt": "GTGATAAAAAAAGTTCAACATGCTTTAATCTTGAATGAATTGACAAAACTGCGTGATAAAAATACAACAACCTCCCAGTTTCGCATGGCCTTGAATCAAATCACTTCATTACTCTTTTTTGAAGCAACTAAACAGCTACCACTAGCAACAGTTGAAGTTGAAACTCCCTTTGCTAAAACAAAGGGCTACAAATTAAAAAATGACATTGTTCTTGTACCTATTATGCGTGCTGGACTTGGAATGATTGATGCTATTGTTCGCTATTCAGATAAAATCAGAGTTGGTCATTTAGGAATCTATCGTCAAACCCAAACAACCAGTGTAATTTCATACTATAAAAAGATGCCTGAAAACATCTCTGATTCACATGTTATTATTCTTGATCCTATGCTTGCTACTGGAACTACATTGTTAACTGCTATTAAATCTATTAAAGAAGATAAACCTATCAAAATTAGTGTTATTGCTATAGTAGCAGCACCTGAAGGAATTAATAAAGTAGAAAAAATGCATCCTCATGTTGATATATTTCTTGCAGCAATTGATGAAAAGTTAAATGACAATAGATACATAATCCCTGGTCTTGGTGATGCTGGGGACCGTTTATTTGGTACTAAATAA", "aa": "MIKKVQHALILNELTKLRDKNTTTSQFRMALNQITSLLFFEATKQLPLATVEVETPFAKTKGYKLKNDIVLVPIMRAGLGMIDAIVRYSDKIRVGHLGIYRQTQTTSVISYYKKMPENISDSHVIILDPMLATGTTLLTAIKSIKEDKPIKISVIAIVAAPEGINKVEKMHPHVDIFLAAIDEKLNDNRYIIPGLGDAGDRLFGTK", "aa_hexdigest": "9ae84fd6e1678687e79f4aa1b2b1c548", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000137D2A", "ncbi_nrp_id": "WP_009885912.1", "uniref100_id": "UniRef100_P47276", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000137D2A", "RefSeq:WP_009885912.1", "UniRef:UniRef100_P47276" ] }, "ips": { "uniref100_id": "UniRef100_P47276", "uniref90_id": "UniRef90_P47276" }, "psc": { "uniref90_id": "UniRef90_P47276", "gene": "upp", "product": "uracil phosphoribosyltransferase", "ec_ids": [ "2.4.2.9" ], "uniref50_id": "UniRef50_P47276", "cog_id": "COG0035", "cog_category": "F", "go_ids": [ "GO:0000287", "GO:0004845", "GO:0005525", "GO:0005737", "GO:0006223", "GO:0044206" ] }, "pscc": { "uniref50_id": "UniRef50_P47276", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47276" ], "product": "Uracil phosphoribosyltransferase" }, "genes": [ "upp" ], "id": "KMADJIBOEG_100", "locus": "GCA000292445_00060" }, { "type": "cds", "sequence": "contig_1", "start": 32354, "stop": 32815, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12836.13", "PFAM:PF14579.12" ], "nt": "TTGAGTTACTATGCTTGTTTATTAAGTTTCAAACTAAAGGAACATGATATTAATGGTTTTGAAAAAGGGTATGAATTTATTAAAAACCGTTTAGATGAACTTAATAAGCTTTACAGAATAAAAAAGATTAAACCAAAAGAAGCGGAGCTTTTAATAAGTTATGAAGTTTATTTAGAAATGATGGCACGCAATATAAAGTTGCAACAAATCTCTATTCAAAACTCTAATGCAAGGATGTTTGTTGAACATAATGGTGTTTTAATTGCGCCTTTCATCACTATTCCAGGAATGGGAGAAGCGGTAGCTTCTTCAATTGTAGAAGCAAGAAATGAAAAACCATTTGCTTCCTTAAATGATTTCAAAAAAAGAACCAAAATTACTAAAAAACACGTTGAAACGATGGAACAATTACAACTGTTTGATGAATTTGAACATCAAGATGATCATAAACTTTTCAACTAA", "aa": "MSYYACLLSFKLKEHDINGFEKGYEFIKNRLDELNKLYRIKKIKPKEAELLISYEVYLEMMARNIKLQQISIQNSNARMFVEHNGVLIAPFITIPGMGEAVASSIVEARNEKPFASLNDFKKRTKITKKHVETMEQLQLFDEFEHQDDHKLFN", "aa_hexdigest": "5d64c3e607f0a0cc5085d8b823cdc9f0", "start_type": "TTG", "rbs_motif": "ATTTA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12836.13", "name": "HHH_3", "length": 44, "aa_cov": 0.28104575163398693, "hmm_cov": 1.0, "evalue": 1.7861084570790182e-09, "score": 32.78656005859375, "start": 93, "stop": 135 }, { "id": "PF14579.12", "name": "HHH_6", "length": 85, "aa_cov": 0.5294117647058824, "hmm_cov": 0.9882352941176471, "evalue": 6.085105789217583e-17, "score": 56.831092834472656, "start": 65, "stop": 145 } ], "seq_stats": { "molecular_weight": 18004.651999999995, "isoelectric_point": 7.817001152038574 }, "id": "KMADJIBOEG_101", "locus": "GCA000292445_00061" }, { "type": "cds", "sequence": "contig_1", "start": 32846, "stop": 33049, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTATTTAATTAATAAAGGGATGCAAGCAAAAGATGCTTTTGAAATCATGGAAAAAGTAAGAAAAGGTATTAAAGTAAATGCTAAAGAAGTTAGCCTGATGCAAAACTGTGGTGTTGAACAATATTGGATTAATGCTTGTTTAAAAATTAATTATCTATTCCCTAAGGCACATGCTGCAGCTTATGTTTTAATGGCTTGA", "aa": "MLYLINKGMQAKDAFEIMEKVRKGIKVNAKEVSLMQNCGVEQYWINACLKINYLFPKAHAAAYVLMA", "aa_hexdigest": "34910a29dfd04a42bf9e5e4df94ceae6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7654.1149000000005, "isoelectric_point": 9.230857276916506 }, "id": "KMADJIBOEG_102", "locus": "GCA000292445_00062" }, { "type": "cds", "sequence": "contig_1", "start": 33125, "stop": 33379, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17657.6" ], "nt": "ATGTTAAAGCACTTAGCTGATCTAACTAAGATAAATCCCCAAAATATCCCTCACTTTGATAAGAACTTAATTTCGATGTTTTCTTCAAACAAACCATTAAACCTAAAACCAGGCATTGTTGATGAAGTTACAGGAGCGGTTGGTATCCCTGAATTTGGGACTAAATTTGTTAGAAAAATTTTAGAACAGACAAAACCTAAAGACTTTGCTGACTTAATTAGAGTTTCAGGATTAAGCCATGGTAAAAACGTGTGA", "aa": "MLKHLADLTKINPQNIPHFDKNLISMFSSNKPLNLKPGIVDEVTGAVGIPEFGTKFVRKILEQTKPKDFADLIRVSGLSHGKNV", "aa_hexdigest": "6a9bef7ad3a19e47ef211790435f60e8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17657.6", "name": "DNA_pol3_finger", "length": 88, "aa_cov": 0.9761904761904762, "hmm_cov": 0.8409090909090909, "evalue": 8.419164329829463e-18, "score": 59.38804626464844, "start": 2, "stop": 83 } ], "seq_stats": { "molecular_weight": 9308.783999999992, "isoelectric_point": 9.630883598327635 }, "id": "KMADJIBOEG_103", "locus": "GCA000292445_00063" }, { "type": "cds", "sequence": "contig_1", "start": 33458, "stop": 33892, "strand": "-", "frame": 3, "gene": null, "product": "DNA polymerase III alpha subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380E0Y6" ], "nt": "ATGATTCGTGATTGTCCTAAATGCCATGAAAAAGCAAGTTTCAAAGGTGATGGTCATAACATTCCTTTTGCTACTTTTATGGGTTTTTCTGGTGATAAAATTCCTGATATCGATCTTAATTTTTCTAGCGAATATCAAGCTAAAGCCCATGATTATGTCAGAAAATTATTTGGAGTTAATAACACCTTCAGGGCAGGAACAATTGCAACAGTTGCTGAAAAAACTGCTTATGGTTATGCTAGAAACTATTTTGAAATCATTAAACGTGTTGATCTTGCAACTACTGCTGAAATTGAAAGATTTAAACAAAAGCTTATAGGAATTAAGAGAACAACAGGGCAACATCCAGGGGGAATTATGATCTTTCCTAGTGATCATTCTGTTTATGAATTTACTCCCTGTGGTTTTCCAGCAGATGATGTTGAAAGTGAATGA", "aa": "MIRDCPKCHEKASFKGDGHNIPFATFMGFSGDKIPDIDLNFSSEYQAKAHDYVRKLFGVNNTFRAGTIATVAEKTAYGYARNYFEIIKRVDLATTAEIERFKQKLIGIKRTTGQHPGGIMIFPSDHSVYEFTPCGFPADDVESE", "aa_hexdigest": "c63ff05b17895e59818e6c4bcc026882", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A380E0Y6", "query_cov": 0.8402777777777778, "subject_cov": 0.9453125, "identity": 0.562, "score": 140.0, "evalue": 3.12e-40, "valid": false, "product": "DNA polymerase III alpha subunit", "uniref50_id": "UniRef50_A0A380E0Y6" }, "pscc": { "uniref50_id": "UniRef50_A0A380E0Y6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380E0Y6" ], "product": "DNA polymerase III alpha subunit" }, "genes": [], "id": "KMADJIBOEG_104", "locus": "GCA000292445_00064" }, { "type": "cds", "sequence": "contig_1", "start": 34190, "stop": 34360, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAAAAGAATGAGTTTTTTAGGAGAAGATATTGCCTATAAATTAGTCGTTGAAAACACTAATAAGATAGCGAAGCTACTTGACTTAAATGAATTAGTACCAACTAAAAACAAGCTTTATCCACCAGTGATGCAAGATTCTAACCAAAAATTAATTGATAAAACTTGA", "aa": "MLKRMSFLGEDIAYKLVVENTNKIAKLLDLNELVPTKNKLYPPVMQDSNQKLIDKT", "aa_hexdigest": "2eb5684ac660461bc551c9e4c2eb60b0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6463.584800000002, "isoelectric_point": 9.049507713317869 }, "id": "KMADJIBOEG_105", "locus": "GCA000292445_00065" }, { "type": "cds", "sequence": "contig_1", "start": 34430, "stop": 35104, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATCCCTTGTTTTCATCCCATACTTTAAGCAATATTTGTTCTAAACTAAAACTTGAATTTGATGATGAAAGATTACACCGTGCTGAATATGATACAGAAGCTTTAAAAAAAGTTTTCTTTTACTTTAAAAAGCAGTTAAAAGAAATGGGTATTAATACCTTAACAGAGATAGATCAAAACCTAAATAAAAAGTGTCAAATTGATCTGATGAAAAGGGTATTTACAAACACAGCAATCGTTTATGTAAAAAACCAACGGGGCTTTCAAAACCTATATGAGATGTTATCAATTGCATTAACAGATCATAATGCTAACAGACCATTAGTTTTAGCTAGCAGCTTAGCTAAATTTAGAAAATCTTTTTTATTAACAGAAAACCCTGTTCAGGGTGACATCTTTAAAGCTGCATTAACAAAACCAATTAATGAACTTGAAAAAGCAATTGAAAAAGTTGACTTTGTGCTTATTTCACAACCCAATGCTTACCTTGGTTATACACTACGTGAAGGACTGAAAAAAGAACTTATTAATGATGCAATCAAACTAGTTATCAAAACAGCAACAAAACTTAAGAAATTAGTTGCTGTTGCATCTGATGCTTACTTTATCCATCCTTGGGAAAATGAATATTATAAGGCAATAGTTTGTGCTAAGGGCTTAGGTGGGAAATGA", "aa": "MNPLFSSHTLSNICSKLKLEFDDERLHRAEYDTEALKKVFFYFKKQLKEMGINTLTEIDQNLNKKCQIDLMKRVFTNTAIVYVKNQRGFQNLYEMLSIALTDHNANRPLVLASSLAKFRKSFLLTENPVQGDIFKAALTKPINELEKAIEKVDFVLISQPNAYLGYTLREGLKKELINDAIKLVIKTATKLKKLVAVASDAYFIHPWENEYYKAIVCAKGLGGK", "aa_hexdigest": "b05e5e06f8842947487e95cec938c3d0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25642.648900000022, "isoelectric_point": 9.30048313140869 }, "id": "KMADJIBOEG_106", "locus": "GCA000292445_00066" }, { "type": "cds", "sequence": "contig_1", "start": 35108, "stop": 35575, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13482.12", "PFAM:PF00929.31" ], "nt": "GTGGCTAAAAAATATGATTTAAAAGCTATCTATGGTTTGGAATTCAACTTAACTGATGATCACATCAAAATTGTTCACAACCCAGATAACACTAAGTTAAGTGATGCCACCTTTGTTATTTTTGATATTGAAACCACTGGATTACATGGTAGGTATGATGATGTTATTGAGTTTTCAGCACGCAAAATTAAGAATAACAGCGAGATAGATCATCAGCAATTCTTTTTAAAAATTGACAAACCTATCCCAAAAACAATCACTGAAATCACCAAAATAACTGATGAGATGCTTGAAGGCGGTATTGATCAACAGCAAGGTTTAGAAAAGATAAGAAATTATCTAGATGATTGTGTTATGGTAGCTCATAATGGTATTAATTTTGATTTACCCTTTTTGCAAACTCAATTTGAAAAATACAACATCAAACCATTAACAAACCCGCTGATTGATACCTTATGTTTATCATGA", "aa": "MAKKYDLKAIYGLEFNLTDDHIKIVHNPDNTKLSDATFVIFDIETTGLHGRYDDVIEFSARKIKNNSEIDHQQFFLKIDKPIPKTITEITKITDEMLEGGIDQQQGLEKIRNYLDDCVMVAHNGINFDLPFLQTQFEKYNIKPLTNPLIDTLCLS", "aa_hexdigest": "049ac24d0929f877f3495ed2137092f8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13482.12", "name": "RNase_H_2", "length": 112, "aa_cov": 0.7225806451612903, "hmm_cov": 0.8125, "evalue": 1.7953544268438253e-09, "score": 32.90215301513672, "start": 39, "stop": 150 }, { "id": "PF00929.31", "name": "RNase_T", "length": 124, "aa_cov": 0.7483870967741936, "hmm_cov": 0.9919354838709677, "evalue": 2.7242022579738147e-25, "score": 84.94076538085938, "start": 39, "stop": 154 } ], "seq_stats": { "molecular_weight": 17877.1915, "isoelectric_point": 5.01811008453369 }, "id": "KMADJIBOEG_107", "locus": "GCA000292445_00067" }, { "type": "cds", "sequence": "contig_1", "start": 35633, "stop": 35722, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAGTTAGTTTTTCATACTAAAATGACTGCTTTTGATGGTATCAATGACATTGAAGAATATGCACAGTTTGCTAAAGAAAGAGGTTGA", "aa": "MELVFHTKMTAFDGINDIEEYAQFAKERG", "aa_hexdigest": "dcdc63ed0322925c47ba882e9f810bcd", "start_type": "GTG", "rbs_motif": "ATTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3391.782, "isoelectric_point": 4.641665458679199 }, "id": "KMADJIBOEG_108", "locus": "GCA000292445_00068" }, { "type": "cds", "sequence": "contig_1", "start": 35897, "stop": 36190, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATGCTGGTTTTAAAAACTTTAACTTCATTGCACTTTATCCTATTGATAAAAATGAATCTAAGTTAAAAGTAAAAGCTGTAACTGTTTCTCAATATGATAAGCAGTTTGAAACAAAGGTATTTGCAACAGAATTTATCCCTATCCACAAGATTAACCAACAGATCGATGATGTCAAGATTATTGGGCAAATCTTTGAATTAAAAACCCATGAAAGTTTAACTGGTAAAAAAACACTTAATATCTATGTAACTGATTTTCAATTAGGTGGATCGTTAATTTTGAAATGA", "aa": "MNNAGFKNFNFIALYPIDKNESKLKVKAVTVSQYDKQFETKVFATEFIPIHKINQQIDDVKIIGQIFELKTHESLTGKKTLNIYVTDFQLGGSLILK", "aa_hexdigest": "429e5eb910b192178112f6fa5c312b6e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11109.755599999991, "isoelectric_point": 9.156976509094239 }, "id": "KMADJIBOEG_109", "locus": "GCA000292445_00069" }, { "type": "cds", "sequence": "contig_1", "start": 36509, "stop": 36709, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTATTTAATCTTGAGAATGAAAAAGATAAAAAGGTTCTTGCACTCTCCAATTTTTTGATAGATAACAATATCCTTGACCACAATGAGCTTAACAGCTTAATTGAAAGGATTGAATCAATTTACTTTAATAAATATATTGAAGAGAAGGTTTTTCTATTTGCAATAACTATTAGTCGCCCTTTAACTATTGATATTTGA", "aa": "MVFNLENEKDKKVLALSNFLIDNNILDHNELNSLIERIESIYFNKYIEEKVFLFAITISRPLTIDI", "aa_hexdigest": "c41b364f725ab4630d87c3c3921fab6b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7815.942699999991, "isoelectric_point": 4.750114250183105 }, "id": "KMADJIBOEG_110", "locus": "GCA000292445_00070" }, { "type": "cds", "sequence": "contig_1", "start": 36974, "stop": 37426, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "ATGAAAACAATTCATAAACTATTTTTAGGTCTTTGTTTACCCGCAACATTAGGTCCTTTACTTGGAATTGTTGTTACAAATACTGACCAAAGTATTAAGTTTACAAGCAAATCAAATTCGATTAATAAGAATAACCAAAATAAAGAGTTGGCTCTACTTAGAGATAATTTGATGAACGAAGCGAAAGTTGATGAACCACTTTCCTTTGAAAAACGGTTTGAAAACTTTAAAAATAAGTATAGTGATATACATAGCTTAAATAACAGTGTTTTTTCACTTCATGACGTTTATGACTTATTAGGTGGATTTAAACAATCATTGACAACATTTTTTGATGAAGTGATTGCCCAACAACAAAAGATCAAGGATGCAGATAAGATCTTTCCAAGTACTAAAGATAATCCACCTAAAGAAGAAAATCCTAATGTTTTAGATACACTAGCTAACTACTAA", "aa": "MKTIHKLFLGLCLPATLGPLLGIVVTNTDQSIKFTSKSNSINKNNQNKELALLRDNLMNEAKVDEPLSFEKRFENFKNKYSDIHSLNNSVFSLHDVYDLLGGFKQSLTTFFDEVIAQQQKIKDADKIFPSTKDNPPKEENPNVLDTLANY", "aa_hexdigest": "887b408f0725611be39ea5161461948d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 87, "aa_cov": 0.58, "hmm_cov": 1.0, "evalue": 6.8615097212935436e-34, "score": 112.35646057128906, "start": 64, "stop": 150 } ], "seq_stats": { "molecular_weight": 17021.131999999994, "isoelectric_point": 6.288290977478028 }, "id": "KMADJIBOEG_111", "locus": "GCA000292445_00071" }, { "type": "cds", "sequence": "contig_1", "start": 37481, "stop": 37954, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "GTGTTCCAAAATTTCACTGATTTTAGGATTAATGACTACAAGATTAAAAATTTTAATGTTGATCTTGTTAGTGAAAATGACATTATTAAACATGATAAAGTTCGTTATGCTTTTGAAGTTAAGTTCAATATTGCTTTAGTTTTATCTATTAATAAGTCAAATGTTGATTTTGACTTTGATTTCATTTTAAAGACTGATAATTTCTCAGACATTGAAAACTTTAATGAAATTTTCAACAGAAAACCTGCTTTACAATTTCGTTTTTATACCAAGATCAATGTACATAAGTTAAGTTTCAATGGTAGTGATTCCACTTACATTGCCAATATCTTGTTACAAGATCAGTTCAACCTATTAGAAATTGATTTAAATAAATCTATTTATGCATTAGATCTTGAAAATGCTAAAGAACGCTTTGATAAGGAATTTGTTCAACCTCTTTATCAAAAACGACGTGAAGCAAAGCTTGCTTGA", "aa": "MFQNFTDFRINDYKIKNFNVDLVSENDIIKHDKVRYAFEVKFNIALVLSINKSNVDFDFDFILKTDNFSDIENFNEIFNRKPALQFRFYTKINVHKLSFNGSDSTYIANILLQDQFNLLEIDLNKSIYALDLENAKERFDKEFVQPLYQKRREAKLA", "aa_hexdigest": "53530dc2c3101994bd1d84973d997fda", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 129, "aa_cov": 0.8152866242038217, "hmm_cov": 1.0, "evalue": 3.0654116403605437e-54, "score": 178.96926879882812, "start": 1, "stop": 128 } ], "seq_stats": { "molecular_weight": 18774.058199999992, "isoelectric_point": 5.726664924621583 }, "id": "KMADJIBOEG_112", "locus": "GCA000292445_00072" }, { "type": "cds", "sequence": "contig_1", "start": 38309, "stop": 38437, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGAGATGATAAAGCATTTCAATATGCTTTTGGTTTAGGTCCAAATACTTCTGAACAACAACTTGGTAGAGTAACCCTACCTGGTTATGGTTATCAAGGAATTAGAATGAGTGATTGGTTGAGATGA", "aa": "MRDDKAFQYAFGLGPNTSEQQLGRVTLPGYGYQGIRMSDWLR", "aa_hexdigest": "cb43104c7f7b924d8d74c300b3dae12c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4824.370500000001, "isoelectric_point": 8.18795223236084 }, "id": "KMADJIBOEG_113", "locus": "GCA000292445_00073" }, { "type": "cds", "sequence": "contig_1", "start": 38434, "stop": 38529, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCATTGGGTTACTTACTAGTTTCACTTTAAGTCCACCTAAAAATGTTGAAGCTAATCTTATAGGTGATGCTAATGATAAAAAACACATTTGA", "aa": "MSIGLLTSFTLSPPKNVEANLIGDANDKKHI", "aa_hexdigest": "b7235956b36badf48068e9532a31829f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3324.8006000000005, "isoelectric_point": 6.508939933776855 }, "id": "KMADJIBOEG_114", "locus": "GCA000292445_00074" }, { "type": "cds", "sequence": "contig_1", "start": 38731, "stop": 38973, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTTACTATAGATTACATACAACTCCACCACAATATCGTTTAACTGATGATATGAAATTATTGTTTGTTCCGCACAGTATCCAAAGGGTAACTGCAGTTGGTAATGAAAGCATTAATGGTCTTCTCAGATCACAAAACCTTCATAACTTGGAACGTCAATCATATGAAGCGACTGCTCCTATTGATTTAATATCATATATGCTTTATGCAATTAGTGATAAAAAACCACCTCAAAAATAA", "aa": "MPYYRLHTTPPQYRLTDDMKLLFVPHSIQRVTAVGNESINGLLRSQNLHNLERQSYEATAPIDLISYMLYAISDKKPPQK", "aa_hexdigest": "553ab238800d5e6fbb73df9d9f67e94f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9288.560799999996, "isoelectric_point": 9.048605155944824 }, "id": "KMADJIBOEG_115", "locus": "GCA000292445_00075" }, { "type": "cds", "sequence": "contig_1", "start": 39171, "stop": 39287, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAGGAACATTTATCCTAATTATCTTTGGTAATGGTGCAGTTGCTCAAGTTAATTTAAAGAAGATGGCTACAAGTGAAACAAAAGCCAAGTTTTTAACAGTTGCACTTACTTGA", "aa": "MIGTFILIIFGNGAVAQVNLKKMATSETKAKFLTVALT", "aa_hexdigest": "bd89cdffd8da509e26c29ed497b8b0be", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4041.860300000001, "isoelectric_point": 10.00041637420654 }, "id": "KMADJIBOEG_116", "locus": "GCA000292445_00076" }, { "type": "cds", "sequence": "contig_1", "start": 39534, "stop": 39686, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATTGTACAAGTCCTAGTGTATTTAACATTCCTAGGAATTTTCTAACTGAATTTATTGCAACTTTAATATTGATAGGTGGAGTTGTTGCTGCTAGTCACTTTCTTCATAACAACCCAAACTCTGTTCCTCCTGGATTTATGGGTCTTTGA", "aa": "MHCTSPSVFNIPRNFLTEFIATLILIGGVVAASHFLHNNPNSVPPGFMGL", "aa_hexdigest": "eca3d48452c9b4b26d7b7b38c841d6e6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5380.227999999999, "isoelectric_point": 6.804616355895996 }, "id": "KMADJIBOEG_117", "locus": "GCA000292445_00077" }, { "type": "cds", "sequence": "contig_1", "start": 39868, "stop": 40164, "strand": "-", "frame": 1, "gene": "tdk", "product": "thymidine kinase", "db_xrefs": [ "COG:COG1435", "COG:F", "EC:2.7.1.21", "GO:0004797", "GO:0005524", "GO:0005829", "GO:0008270", "GO:0046104", "GO:0071897", "KEGG:K00857", "SO:0001217", "UniRef:UniRef50_P47280", "UniRef:UniRef90_P47280" ], "nt": "GTGATTATTTCTGGGCTTGATACTGATTTTAGAGCTGAGCCTTTTGGTTGTATTCCCCAACTTTTAGCAATAGCTGATGTAGTTAATAAACTTGATGCTATTTGCAATGTTTGTGGTAGTTTAGCACAACGAACCCAAAGACTAGTAAATAAAAATACTAATGATAACCTTGTCTTAATTGGTGATGCAGAAGCTTATGAAGCTAGATGCAAACTGCACCACTCATTTTTAACTAAAAAGCACGTTACTGTTAAAACCAAAAACTTTAAAGAACAGGTGCAGGAGAAAACCCAATAA", "aa": "MIISGLDTDFRAEPFGCIPQLLAIADVVNKLDAICNVCGSLAQRTQRLVNKNTNDNLVLIGDAEAYEARCKLHHSFLTKKHVTVKTKNFKEQVQEKTQ", "aa_hexdigest": "6af524bf9cdb845509c495dd86c44995", "start_type": "GTG", "rbs_motif": null, "genes": [ "tdk" ], "truncated": "3-prime", "pseudogene": { "start": 39868, "stop": 40464, "observations": { "mutation": [ 40401 ], "stop": [ 40401 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47280", "query_cov": 1.0, "subject_cov": 0.460093896713615, "identity": 0.98, "score": 190.0, "evalue": 8.69e-60, "gene_start": 1, "gene_end": 98, "reference_start": 116, "reference_end": 213, "reference_sequence": "MGKYQPSFQTKKGWTEVICGPMFSGKTEKLLHKIKRWKIAKISVVIFKPIIDTRQTNIVKSRNGEYDQAITINSPFEIYDHLVDKNYQIVAIDEAQFFSNEIIEVVTTLNEIGTNVIISGLDTDFRAEPFGCIPQLLAIADVVNKLDAICNVCGSLAQRTQRLVNKNTNDNLVLIGDAEAYEARCKLHHSFLTKKHVTVKTKNFKEQVQGKTQ" }, "paralog": false, "identity": 0.98989898989899, "score": 388.0, "evalue": 3.58e-140, "description": "Internal stop codon at 40401. Nonsense mutation around 40401.", "psc": { "uniref90_id": "UniRef90_P47280", "gene": "tdk", "product": "thymidine kinase", "ec_ids": [ "2.7.1.21" ], "uniref50_id": "UniRef50_P47280", "cog_id": "COG1435", "cog_category": "F", "kegg_orthology_id": "K00857", "go_ids": [ "GO:0004797", "GO:0005524", "GO:0005829", "GO:0008270", "GO:0046104", "GO:0071897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47280", "EC:2.7.1.21", "UniRef:UniRef50_P47280", "COG:COG1435", "COG:F", "KEGG:K00857", "GO:0004797", "GO:0005524", "GO:0005829", "GO:0008270", "GO:0046104", "GO:0071897" ] }, "pscc": { "uniref50_id": "UniRef50_P47280", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47280" ], "product": "Thymidine kinase" } }, "id": "KMADJIBOEG_118", "locus": "GCA000292445_00078" }, { "type": "cds", "sequence": "contig_1", "start": 40538, "stop": 40915, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30", "PFAM:PF01409.25", "PFAM:PF13393.11" ], "nt": "ATGAACTTCTTGCAAAAACCAAGGGGAGTTAAAGATTGGTTTGGTGATGAATTAGTTTATTTTAATTGGATTGTTAAAAAAATAAGATCTTTAGCATTTAATTGGGGTTTTAGTGAAGTTAAAACTCCGTTGTTTGAAAATGCACAACTTTTTCAAAGATCTAATGCTAATGCTGATATTGTTCAAAAAGAACTATACCAGTTTTTTGATAAATCTCAAAGAGAATTAGCTTTAAGACCTGAAGCTACTACACCAATAGTAAGACTTGCTTGTGAAAACAAATTAATGCAAGAAGCAAATTTTCCCTTAAAGTTATTTTGCATTGGTTCAATGTATCGTTATGAACGTCCACAAAACAATAGGTTTCGTGAACATTGA", "aa": "MNFLQKPRGVKDWFGDELVYFNWIVKKIRSLAFNWGFSEVKTPLFENAQLFQRSNANADIVQKELYQFFDKSQRELALRPEATTPIVRLACENKLMQEANFPLKLFCIGSMYRYERPQNNRFREH", "aa_hexdigest": "c6e29bd97c9b27caa40ee2735951fe46", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 55, "aa_cov": 0.432, "hmm_cov": 0.9818181818181818, "evalue": 3.1303436581437845e-10, "score": 35.518009185791016, "start": 66, "stop": 119 }, { "id": "PF01409.25", "name": "tRNA-synt_2d", "length": 101, "aa_cov": 0.752, "hmm_cov": 0.9504950495049505, "evalue": 7.808687985128238e-07, "score": 23.916473388671875, "start": 23, "stop": 116 }, { "id": "PF13393.11", "name": "tRNA-synt_His", "length": 115, "aa_cov": 0.92, "hmm_cov": 0.9478260869565217, "evalue": 4.8720642379335276e-17, "score": 57.226783752441406, "start": 10, "stop": 124 } ], "seq_stats": { "molecular_weight": 15002.076399999987, "isoelectric_point": 9.389449501037596 }, "id": "KMADJIBOEG_119", "locus": "GCA000292445_00079" }, { "type": "cds", "sequence": "contig_1", "start": 40931, "stop": 41065, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGGTTTTTCCAACCTGTTTATCTTTTTAGATACACTTTTGTTTGCTAACTCTTTGCTTGAAGCACTTGGAATTACTGGATATGTGCTTAAAATTAATAATCTTGCTAACTTTGAAACACTTAGTAAGTGA", "aa": "MFGFSNLFIFLDTLLFANSLLEALGITGYVLKINNLANFETLSK", "aa_hexdigest": "232fa0335f19255ddff613feaef7442f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4914.711299999999, "isoelectric_point": 4.677076148986816 }, "id": "KMADJIBOEG_120", "locus": "GCA000292445_00080" }, { "type": "cds", "sequence": "contig_1", "start": 41147, "stop": 41293, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGAATTTTAGATGACAAGATAGATCAAAAAAAATCATTTGTTAAAAATGCTCCTAAAATTACTGATTTTTTAGATGCAAGTGCAAAACAAGATTCAGAATTGTTAAAAACACAACTAAAAAAACACAATATTAGTTTTGAATGA", "aa": "MRILDDKIDQKKSFVKNAPKITDFLDASAKQDSELLKTQLKKHNISFE", "aa_hexdigest": "e9f398681deccbdb36eac70c9c1b963f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5577.368899999999, "isoelectric_point": 9.10056667327881 }, "id": "KMADJIBOEG_121", "locus": "GCA000292445_00081" }, { "type": "cds", "sequence": "contig_1", "start": 41315, "stop": 41563, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13393.11" ], "nt": "TTGGATTACTATACTGGATTTGTGTTTGAATATGTAAAAAATCAAGACACAATTTTAGCAGGTGGAGTTTATGATAACTTAGTTGAAGAATTAAGTAGTAATCCAACTCCCGCATTAGGTTTTGCTTGTGGAATTGAACGGTTAATTAACTGTTTAGAAATTGATAAAAAAGCATTTATTTTGAATACTAAACCAAAGCAGATGTTAGTAATTTGCTTATTTGAAGAAGCGCTTGAAGAATTGGTTTGA", "aa": "MDYYTGFVFEYVKNQDTILAGGVYDNLVEELSSNPTPALGFACGIERLINCLEIDKKAFILNTKPKQMLVICLFEEALEELV", "aa_hexdigest": "5b2c62c773554382779f3964497e95a3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13393.11", "name": "tRNA-synt_His", "length": 49, "aa_cov": 0.573170731707317, "hmm_cov": 0.9795918367346939, "evalue": 2.7707809961386863e-10, "score": 35.041114807128906, "start": 2, "stop": 48 } ], "seq_stats": { "molecular_weight": 9253.618999999997, "isoelectric_point": 4.2809652328491214 }, "id": "KMADJIBOEG_122", "locus": "GCA000292445_00082" }, { "type": "cds", "sequence": "contig_1", "start": 42348, "stop": 42884, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGATTGCAGGAGTTGAAAAATATTTTCAAATTGCAAGGGTCTATCGTGATGAAGATAGCAGAAAAGATCGTCAACCAGAACACACACAAATTGATTTCGAGATCTCTTTTTGTAACCAAAAAATGATTATGAATCTAGTTGAAAAACTCTTTTTTAGTGTTTTCTTAGATGTTTTTCAAATCAAAATAAAAAAGACTTTTCCTGTTTTTAAATTTTCAGAACTTTTTGAAAGATTTGGTAGCGATAAACCAGATTTACGTTATGGTTTTGAAATAAAAGATTTCACCTCGCTTTTTCAAGATCATCAGAATCAGTTCACTAAATTAATTGAAGCAAAAGGCATTATTGGTGGTATTGAACTTACTAATATTGAGTTAAGTACAGACAAAATTAAAGCATTAAGAAAAATTGCTAAGGACCATGATGTGAGTTTAGAAGTTCATAATAAAAATAATTCAACATTAAAAACTTCAATTAAATGTGATGAAAAAAACACTCTTCTGTTAGTAGCAAATAAATCTAAAAAGAAGGCATGA", "aa": "MIAGVEKYFQIARVYRDEDSRKDRQPEHTQIDFEISFCNQKMIMNLVEKLFFSVFLDVFQIKIKKTFPVFKFSELFERFGSDKPDLRYGFEIKDFTSLFQDHQNQFTKLIEAKGIIGGIELTNIELSTDKIKALRKIAKDHDVSLEVHNKNNSTLKTSIKCDEKNTLLLVANKSKKKA", "aa_hexdigest": "02319725e1814c0aaee1acb097fea956", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 115, "aa_cov": 0.6179775280898876, "hmm_cov": 0.8608695652173913, "evalue": 2.8000364234635463e-30, "score": 100.53925323486328, "start": 1, "stop": 110 } ], "seq_stats": { "molecular_weight": 20805.805700000023, "isoelectric_point": 9.081935310363772 }, "id": "KMADJIBOEG_123", "locus": "GCA000292445_00083" }, { "type": "cds", "sequence": "contig_1", "start": 43176, "stop": 43400, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGAATTCTTTGAACATTGACCCAAATAAGTTTGCTTTTCTTCTAGAAGCTTATGAATATGGTGCTCCTGTTCATGGTGGAATGGGACTAGGTATTGATCGTTTAATGATGATTCTTAATCAAACTGATAACATTAGAGAAGTAATCGCTTTTCCTAAGAATAATCATGGTATTGAAGTCCATACAAACGCTCCTGATAAAATTGACAAAGAGGAGGTTAAATGA", "aa": "MNSLNIDPNKFAFLLEAYEYGAPVHGGMGLGIDRLMMILNQTDNIREVIAFPKNNHGIEVHTNAPDKIDKEEVK", "aa_hexdigest": "3bd48fffb316d119fabe1896e9494b35", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 52, "aa_cov": 0.6486486486486487, "hmm_cov": 1.0, "evalue": 5.750666164848798e-20, "score": 66.66931915283203, "start": 7, "stop": 54 } ], "seq_stats": { "molecular_weight": 8309.404899999998, "isoelectric_point": 5.147191429138182 }, "id": "KMADJIBOEG_124", "locus": "GCA000292445_00084" }, { "type": "cds", "sequence": "contig_1", "start": 43400, "stop": 43561, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF18127.6" ], "nt": "ATGGATAAAAGAACTAGTGAAATAGAGTTTCACTTAAAGAATCTTTTAGCTTGTGATGCATATAAACTTTCACACCGTTTAATGTATCCACAAGATACACAAAACCTTTATAGTATGTTAACTGCAAGAGGTGTTTATGGTGATTTTAAGGAGTTTGTTTGA", "aa": "MDKRTSEIEFHLKNLLACDAYKLSHRLMYPQDTQNLYSMLTARGVYGDFKEFV", "aa_hexdigest": "1a29b07947f892e4796a2ab69a699fd3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF18127.6", "name": "NAMPT_N", "length": 37, "aa_cov": 0.6981132075471698, "hmm_cov": 0.972972972972973, "evalue": 5.423485731357371e-07, "score": 25.006837844848633, "start": 15, "stop": 51 } ], "seq_stats": { "molecular_weight": 6289.158400000001, "isoelectric_point": 6.702988243103028 }, "id": "KMADJIBOEG_125", "locus": "GCA000292445_00085" }, { "type": "cds", "sequence": "contig_1", "start": 43642, "stop": 44253, "strand": "+", "frame": 1, "gene": "pncB", "product": "nicotinate phosphoribosyltransferase", "db_xrefs": [ "COG:COG1488", "COG:H", "EC:2.4.2.12", "EC:6.3.4.21", "GO:0004514", "GO:0009435", "GO:0047280", "SO:0001217", "UniRef:UniRef50_P75067", "UniRef:UniRef90_P47283" ], "nt": "TTGCTAGCTGCAGCATTGACAGATAAATTAGTTAGTGTTTTTAATGATCATGAATTGGCTAATGAATTCACACAACACATCTGTCATTTAGCTAGTTTCTTAGAAAAAAATAAAAAAATGCCGTTAGTTGCAAAGATTCATGAAAGTGATCAATCATTACCATTTAGAACTCCTTTAATAACTATAGAAGGAGTTGAAAATATTCCAAGCAACTTTGTATGGTTAGTTAATTACTTTGAAACTGTACTTTTAGAAAATATTTGGTTGTTTCAAACTGCTTCTACAGTTGCTAAAAGAATTAAATCTTTACTTGAAAAATATGCTAAAGAAACCGCAGATGAAACAAGTTTTATTAATTTTCAATGCCACGACTTTAGTATGCGGGGCATGAGTAGTTTGCAAAGTGCTTTGTATGTTGCTAGAGCACACTTGCAATACTTTACTGGAAGTGACACGATCTTAGGTGGGGATAATTCTCGTTCAATTTTAGCTTCTGAACATTCAGTGATGTGCGCAGATGGTAGTAAACATGAATTGAAAACTTTTCAACGTTTATTGGAAAAGTTTAAAGATAAAAAACTTTCTTTAGTGATTGATTCTTATGACATGTGA", "aa": "MLAAALTDKLVSVFNDHELANEFTQHICHLASFLEKNKKMPLVAKIHESDQSLPFRTPLITIEGVENIPSNFVWLVNYFETVLLENIWLFQTASTVAKRIKSLLEKYAKETADETSFINFQCHDFSMRGMSSLQSALYVARAHLQYFTGSDTILGGDNSRSILASEHSVMCADGSKHELKTFQRLLEKFKDKKLSLVIDSYDM", "aa_hexdigest": "283e5d590aa90e7d3d46a3417bff7b5a", "start_type": "TTG", "rbs_motif": null, "genes": [ "pncB" ], "truncated": "both", "pseudogene": { "start": 43397, "stop": 44556, "observations": { "deletion": [ 43634 ], "mutation": [ 43559 ], "stop": [ 43559, 44252, 44393 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47283", "query_cov": 1.0, "subject_cov": 0.45111111111111113, "identity": 0.99, "score": 400.0, "evalue": 2.33e-137, "gene_start": 1, "gene_end": 203, "reference_start": 83, "reference_end": 285, "reference_sequence": "MMDKRTSEIEFHLKNLLACDAYKLSHRLMYPQDTQNLYSMLTARGVYGDFKEFVWNHDFAKEILLNVFNGFVNSVIEVKKNKLLAAALTDKLVSVFNDHELANEFTQHICHLASFLEKNKKMPLVAKIHESDQSLPFRTPLITIEGVENIPNNFVWLVNYFETVLLENIWLFQTASTVAKRIKSLLEKYAKETADETSFINFQCHDFSMRGMSSLQSALYVARAHLQYFTGSDTILGGDNSRSILASEHSVMCADGSKHELKTFQRLLEKFKDKKLSLVIDSYDMWNVLDNIIPRLKNLILMRGATLYLRADSGNYQTLICNPNYKKQDKSTWAMIDYLDHHFSSTINKKGYKVLNKKIGIIYGDGITYQKIEWILNCLKNHGYCSSNIIFGVGSSTYQNLNRDTLGFVYKLTAIKRNNRWIGVKKTPITDLSKSSKGGRYKTKRLITVY" }, "paralog": false, "identity": 0.9818652849740933, "score": 754.0, "evalue": 1.96e-279, "description": "Internal stop codon at 43559, 44252, 44393. Frameshift due to deletion around 43634. Nonsense mutation around 43559.", "psc": { "uniref90_id": "UniRef90_P47283", "gene": "PncB", "product": "nicotinate phosphoribosyltransferase", "ec_ids": [ "2.4.2.12", "6.3.4.21" ], "uniref50_id": "UniRef50_P75067", "cog_id": "COG1488", "cog_category": "H", "go_ids": [ "GO:0004514", "GO:0009435", "GO:0047280" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47283", "EC:2.4.2.12", "EC:6.3.4.21", "UniRef:UniRef50_P75067", "COG:COG1488", "COG:H", "GO:0004514", "GO:0009435", "GO:0047280" ] }, "pscc": { "uniref50_id": "UniRef50_P75067", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75067" ], "product": "Nicotinamide phosphoribosyltransferase" } }, "id": "KMADJIBOEG_126", "locus": "GCA000292445_00086" }, { "type": "cds", "sequence": "contig_1", "start": 44299, "stop": 44394, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTGATGCTACGCTTTATTTGCGTGCTGATTCTGGTAATTATCAAACTCTTATTTGCAATCCTAATTACAAAAAGCAAGATAAAAGTACATGA", "aa": "MRDATLYLRADSGNYQTLICNPNYKKQDKST", "aa_hexdigest": "e41c7ccbd225fa43d481994fe6fdcf37", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3609.009500000001, "isoelectric_point": 8.978205680847168 }, "id": "KMADJIBOEG_127", "locus": "GCA000292445_00087" }, { "type": "cds", "sequence": "contig_1", "start": 44398, "stop": 44748, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCGATTACTTAGATCATCATTTCAGTTCAACTATAAATAAAAAAGGTTATAAGGTTTTAAACAACAAAATTGGAATTATTTATGGTGATGGAATCACCTATCAAAAGATAGAATGGATCTTAAATTGTTTAAAAAACCATGGTTATTGTTCTTCAAACATTATTTTTGGAGTTGGTAGTAGCACTTATCAAAATTTAAACCGTGATACTTTAGGTTTTGTATACAAATTAACTGCTATTAAAAAAAGTAATAGATGGATAGGCGTTAAAAAAACTCCCATAACTGATCTATCTAAAAGTTCAAAAGGCGGTAGATATAAAACAAAGAGATTAATTACAGTTTATTAA", "aa": "MIDYLDHHFSSTINKKGYKVLNNKIGIIYGDGITYQKIEWILNCLKNHGYCSSNIIFGVGSSTYQNLNRDTLGFVYKLTAIKKSNRWIGVKKTPITDLSKSSKGGRYKTKRLITVY", "aa_hexdigest": "65f9e3b17fd0d0e2749e34de59bf488a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13259.220399999978, "isoelectric_point": 9.869481086730957 }, "id": "KMADJIBOEG_128", "locus": "GCA000292445_00088" }, { "type": "cds", "sequence": "contig_1", "start": 44886, "stop": 45065, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02782.22" ], "nt": "ATGGCAAGTGATATAGGTTATAAGATTACTAGCATTAAAGCTGATGGGGGGATTGTTAAATCAAACTATTTAATGCAGTTTCAAGCTGATATTGCAGATGTAATTGTTTCTATCCCTAAAAATAAAGAAACCACTGCAGTTGGTGTTTGTTTTTTAGCTGGACTTGCTTGTGGATTTTGA", "aa": "MASDIGYKITSIKADGGIVKSNYLMQFQADIADVIVSIPKNKETTAVGVCFLAGLACGF", "aa_hexdigest": "123f07ce7d0e33d510412f30b14352eb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02782.22", "name": "FGGY_C", "length": 53, "aa_cov": 0.8983050847457628, "hmm_cov": 1.0, "evalue": 1.2318762557079062e-12, "score": 43.11842346191406, "start": 4, "stop": 56 } ], "seq_stats": { "molecular_weight": 6185.1698, "isoelectric_point": 5.950326347351075 }, "id": "KMADJIBOEG_129", "locus": "GCA000292445_00089" }, { "type": "cds", "sequence": "contig_1", "start": 45183, "stop": 45335, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGTAGCGGGTGCGGCTATAAAATGGTTAAGGGATGCATTAAAAATTATCTATTCAGAAAAGGAAAGTGATTTTTATGCAGAACTTGCAAAAGAAAATGAACAAAACCTAGTTTTTGTACCAGCTTTCAGTGGACTTGGAGCTCCTTGA", "aa": "MFVAGAAIKWLRDALKIIYSEKESDFYAELAKENEQNLVFVPAFSGLGAP", "aa_hexdigest": "8fa1bd9ff07e691a8f53295fd4c1430b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5549.310100000001, "isoelectric_point": 4.7668249130249025 }, "id": "KMADJIBOEG_130", "locus": "GCA000292445_00090" }, { "type": "cds", "sequence": "contig_1", "start": 45601, "stop": 45861, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAACTCAGAACTTTAGGTAAGATTGAAACTGGTACTTCAAATAAATCACATAACTCTTTGGATCACTCCATTTTGACAATGTCAAATAAAAGAGTTCTTGAAGCATTTGAAACATCTGTAACATGCATTTTTCCATTAGTTAGTTTTCAGATTAATCAGCTATCAATGGTGCCAAATAACAACTTTTTTTGCTCCATTAGTTTCTTTGCTAAAGGAACATTTTTTAAGATTCAAGCTATCTTAGTAGCACTAAAATAG", "aa": "MELRTLGKIETGTSNKSHNSLDHSILTMSNKRVLEAFETSVTCIFPLVSFQINQLSMVPNNNFFCSISFFAKGTFFKIQAILVALK", "aa_hexdigest": "36179cddd9d56581e09050ccd2654431", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9647.159099999992, "isoelectric_point": 9.18437557220459 }, "id": "KMADJIBOEG_131", "locus": "GCA000292445_00091" }, { "type": "cds", "sequence": "contig_1", "start": 45999, "stop": 46088, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAGTGCTAAAAATAAAGCACAAATCAAATCTCATGAAGTGATTGCAGTTGGTATTACCAATCAAAGAGAAACAATAGTTTTATGA", "aa": "MQSAKNKAQIKSHEVIAVGITNQRETIVL", "aa_hexdigest": "159464c92f69e0a07a6228989c9a85cf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3207.7024, "isoelectric_point": 9.697157096862792 }, "id": "KMADJIBOEG_132", "locus": "GCA000292445_00092" }, { "type": "cds", "sequence": "contig_1", "start": 46134, "stop": 46271, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCTAAAAAAACAATACATCATTGCCTTAGATGAAGGTACTAGTTCTTGTCGATCAATTGTTTTTGATCACAATCTTAACCAAATAGCAATAGCACAAAACGAATTTAACACTTTTTTTCCTAATAGTGGTTGA", "aa": "MDLKKQYIIALDEGTSSCRSIVFDHNLNQIAIAQNEFNTFFPNSG", "aa_hexdigest": "355f63ae6572bcf6c847359ea0119fdc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5091.643299999999, "isoelectric_point": 4.8263353347778315 }, "id": "KMADJIBOEG_133", "locus": "GCA000292445_00093" }, { "type": "cds", "sequence": "contig_1", "start": 46292, "stop": 46666, "strand": "-", "frame": 3, "gene": null, "product": "Glycerol-3-phosphate dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B2Q2" ], "nt": "ATGGTACCTACTATCCATGGTAAGGGAGTTGTTGTTGCTGAGATGTTAGATGGTAATATTCTTGTGGGACCTAATGCAGTTGAGGGAATTGAAAAAAACAAAACTCGTTCCATAGATTTAGATTCAATTAACCAAATTAAAACTATTGGTAAAAAAATGGTTCCTTCATTGCAATTTGAAAATAGTATTTATAGTTTTGCAGGTTCAAGAGCTATTGATATTGAAACAAATGACTTTGTTATTAGAACAGCAAAAAGCAATCCTAACTTTATCATCCTTGGTGGCATGAAATCCCCTGGCTTAACATCAAGTCCTGCTATTGCTAAGAGAGCAGTTGAACTCCTTAATTTAAAGTTAAAAAAGAAGATAAATTGA", "aa": "MVPTIHGKGVVVAEMLDGNILVGPNAVEGIEKNKTRSIDLDSINQIKTIGKKMVPSLQFENSIYSFAGSRAIDIETNDFVIRTAKSNPNFIILGGMKSPGLTSSPAIAKRAVELLNLKLKKKIN", "aa_hexdigest": "e7198bb38adc51876e45e2edfef62437", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FF40EBC", "query_cov": 0.9919354838709677, "subject_cov": 0.8601398601398601, "identity": 0.561, "score": 130.0, "evalue": 1.02e-36, "valid": false, "product": "FAD-dependent oxidoreductase", "uniref50_id": "UniRef50_A0A449B2Q2" }, "pscc": { "uniref50_id": "UniRef50_A0A449B2Q2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B2Q2" ], "product": "Glycerol-3-phosphate dehydrogenase" }, "genes": [], "id": "KMADJIBOEG_134", "locus": "GCA000292445_00094" }, { "type": "cds", "sequence": "contig_1", "start": 46970, "stop": 47104, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAAAGAAAGGGGAATTAAAAACTCAATTCCTGTTGAAAACATTCAAATATTAGACCAACAACAAACTTTATTGCAAGAACCGTTTATCAATCCAAATGTAGTAGCTAGTTTAAAAGTTGAAGGTAGTTGA", "aa": "MLKERGIKNSIPVENIQILDQQQTLLQEPFINPNVVASLKVEGS", "aa_hexdigest": "e895ac8fa1d30d23f4d04a1af7da3792", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4932.6494, "isoelectric_point": 5.002479362487793 }, "id": "KMADJIBOEG_135", "locus": "GCA000292445_00095" }, { "type": "cds", "sequence": "contig_1", "start": 47198, "stop": 47416, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01266.30", "PFAM:PF13450.12", "PFAM:PF00070.33" ], "nt": "ATGCAAACAATTGACGTTTTAATTGTGGGTGGGGGTATCATCGGTACTAGTTGTGCATATGAACTCAGCCAATATGAATTAAAAGTTGCACTTTTAGAAAAAAATGCTTTTTTGGGTTGTGAAACTTCGCAGGCTAACAGTGGTGTAATCCATTCAGGAATTGATCCTAATCCTAATAAGCTAACTGCCAAATACAACATCTTAGGGAGAAAAATCTGA", "aa": "MQTIDVLIVGGGIIGTSCAYELSQYELKVALLEKNAFLGCETSQANSGVIHSGIDPNPNKLTAKYNILGRKI", "aa_hexdigest": "49554420b7ad75dbe90fe35dfe4342bb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01266.30", "name": "DAO", "length": 60, "aa_cov": 0.8055555555555556, "hmm_cov": 1.0, "evalue": 3.3589331110468356e-20, "score": 67.97188568115234, "start": 5, "stop": 62 }, { "id": "PF13450.12", "name": "NAD_binding_8", "length": 36, "aa_cov": 0.5, "hmm_cov": 1.0, "evalue": 7.648067783549001e-10, "score": 34.14286804199219, "start": 8, "stop": 43 }, { "id": "PF00070.33", "name": "Pyr_redox", "length": 31, "aa_cov": 0.4305555555555556, "hmm_cov": 1.0, "evalue": 3.1477547785511095e-06, "score": 22.800899505615234, "start": 6, "stop": 36 } ], "seq_stats": { "molecular_weight": 7680.7864, "isoelectric_point": 6.527867317199706 }, "id": "KMADJIBOEG_136", "locus": "GCA000292445_00096" }, { "type": "cds", "sequence": "contig_1", "start": 47575, "stop": 47850, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAAGCTATTATTTGGTAAGTTAGTTTTTAAAAAGAGCTTGTTTTTACTGAGTGGTATGAGCAGTTTAGCAGTTTTTTTAACTGCATGCGGGGCTACCAAAATATTTGATTCCTCTGTACAGCTACTAGTATCAGATAACTTTTCCACACTTGCTGATAAATCATTTTCACAAATGTCCTATGAAGGAATCAGGAGCTTTTTTAAAAAAAGTAAGGGTGTTGATTTACCTGAAGCAGATAGTTCACAATTACAAGAAGGCAATGGCTTGTGA", "aa": "MGKLLFGKLVFKKSLFLLSGMSSLAVFLTACGATKIFDSSVQLLVSDNFSTLADKSFSQMSYEGIRSFFKKSKGVDLPEADSSQLQEGNGL", "aa_hexdigest": "8af69b5d4bd6c278f255f2095abea66e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9840.283899999993, "isoelectric_point": 8.818388557434083 }, "id": "KMADJIBOEG_137", "locus": "GCA000292445_00097" }, { "type": "cds", "sequence": "contig_1", "start": 48328, "stop": 48924, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTTATAAACATTTAAGCGATGCACAAGATTCATCTGCACAAGTGACCACTTCTAAACAAACTGTTTTAAAACAACTTCAAGTTGCAAATGGTGAAAAAAAGATTAAAAAGATTAAATGGATTTCACCAAAACAAGGAAGTGATGGAGAAACTATAAACATTCAAGATCACCAATCAGGTTCTTTTTCAGATACTGAACCTAGAGCAATAACAATAGCTAATAATTTAATTGATAAAGGAATTAATGCTATCATTCCTATTGCTGGACCACAAACGAATTTAGTGGTTACTCAAATTGCTAGAAGACAAGCCCATACTGCAGTTATTGGCGTTGATAGTGCACAGGAATTGCTAGATATTAATATTGATGCTCCAAATAAAGATAAGTTAAAAATGGGGAATAAAAAGATTATTCCCTTCTCTTCTATTAAGGCTTTGGATGTTGCTGTTGAAAGTATCTTATCAACATTAGAAAAAGGTTCCAGTCAAAATGGTTATCAGGGCTTTGGATATAACAACATAGGTACAGTAAAAAACAACTCTGTTGGGGTTAGTGAAGCAGGTTATGAATTTTTAATAGATCCTGTTTTTTGA", "aa": "MIYKHLSDAQDSSAQVTTSKQTVLKQLQVANGEKKIKKIKWISPKQGSDGETINIQDHQSGSFSDTEPRAITIANNLIDKGINAIIPIAGPQTNLVVTQIARRQAHTAVIGVDSAQELLDINIDAPNKDKLKMGNKKIIPFSSIKALDVAVESILSTLEKGSSQNGYQGFGYNNIGTVKNNSVGVSEAGYEFLIDPVF", "aa_hexdigest": "1bae88205bf5b09cd089efe4b2f95e11", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21377.959099999985, "isoelectric_point": 7.869736289978027 }, "id": "KMADJIBOEG_138", "locus": "GCA000292445_00098" }, { "type": "cds", "sequence": "contig_1", "start": 48940, "stop": 49185, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGCTATGTCTTTATCAGCAAGTCTAAAAGCTAATGCAGCATCTTCATCAGATAATAAGAAAAAATTATCAGAAGTTGCTACTAAGAAAAATGAAAACGGTTCGACAAAAAATGGTAGTAATGGCATCATTGACAAATATGCCAAACTCTTAACAAAATCTAGTTCTTCAACTAGCATGAGAAACGGTAGTTCAGATAGCAATCAACAGAATTTTAAAACAACAGATAATGATGGTGATTGA", "aa": "MQAMSLSASLKANAASSSDNKKKLSEVATKKNENGSTKNGSNGIIDKYAKLLTKSSSSTSMRNGSSDSNQQNFKTTDNDGD", "aa_hexdigest": "27ab0c851a5a029a78ff812b2b7247cb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8523.170799999996, "isoelectric_point": 9.522834587097169 }, "id": "KMADJIBOEG_139", "locus": "GCA000292445_00099" }, { "type": "cds", "sequence": "contig_1", "start": 49371, "stop": 49637, "strand": "+", "frame": 3, "gene": "ptsH", "product": "Phosphocarrier protein HPr", "db_xrefs": [ "COG:COG1925", "COG:TG", "GO:0005737", "GO:0009401", "RefSeq:WP_014894114.1", "SO:0001217", "UniParc:UPI00027B3643", "UniRef:UniRef100_UPI00027B3643", "UniRef:UniRef50_P75061", "UniRef:UniRef90_P47287" ], "nt": "ATGAAAAAATTTCAAGCAGTTATTAAAGACCCAGTGGGAATTCACGCACGTCCTGCTTCTATCCTTACAAGTGAGGCTAGTAAGTTTAAATCTGAACTTAAACTGGTAGCTCCAAGTGGTGTTGAAGGTAATATTAAATCAATCATTAACTTAATGTCTTTAGGAATTAGACATAATGACAACATTACTATCAAAGCAGATGGAGCTGATGAAGAAGAAGCTTTAGCAGCTATTAAAGCTTGTCTTGAAAAAAATAAAGTTATCTAA", "aa": "MKKFQAVIKDPVGIHARPASILTSEASKFKSELKLVAPSGVEGNIKSIINLMSLGIRHNDNITIKADGADEEEALAAIKACLEKNKVI", "aa_hexdigest": "967e33553ec136711de0e7638a8f44ef", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3643", "ncbi_nrp_id": "WP_014894114.1", "uniref100_id": "UniRef100_UPI00027B3643", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3643", "RefSeq:WP_014894114.1", "UniRef:UniRef100_UPI00027B3643" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3643", "uniref90_id": "UniRef90_P47287" }, "psc": { "uniref90_id": "UniRef90_P47287", "gene": "ptsH", "product": "Phosphocarrier protein HPr", "uniref50_id": "UniRef50_P75061", "cog_id": "COG1925", "cog_category": "TG", "go_ids": [ "GO:0005737", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P75061", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75061" ], "product": "Phosphocarrier protein HPr" }, "genes": [ "ptsH" ], "id": "KMADJIBOEG_140", "locus": "GCA000292445_00100" }, { "type": "cds", "sequence": "contig_1", "start": 49836, "stop": 50159, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920RY59" ], "nt": "TTGAACGAACATTCTTTAATTGAAATTGAAGGTTTGAACAAGACCTTTGATGATGGTTATGTTTCTATAAGAGACATTAGCCTAAATATTAAAAAAGGCGAATTTATTACTATTTTAGGCCCTTCTGGTTGTGGTAAAACTACCCTGTTGAGGTTATTAGCTGGATTTGAAGATCCTACTTATGGCAAGATCAAAGTTAATGGTATTGACATTAAAGACATGGCAATCCATAAGCGTCCTTTTGCGACAGTTTTTCAAGACTATGCTTTATTTTCCCATCTAACTGTTTATAAAAACATTGCTTATGGTCTGAAGGTAATGTGA", "aa": "MNEHSLIEIEGLNKTFDDGYVSIRDISLNIKKGEFITILGPSGCGKTTLLRLLAGFEDPTYGKIKVNGIDIKDMAIHKRPFATVFQDYALFSHLTVYKNIAYGLKVM", "aa_hexdigest": "afb91b292ce699a39b0de5f268b09fee", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A920RY59", "query_cov": 0.9719626168224299, "subject_cov": 0.9541284403669725, "identity": 0.519, "score": 112.0, "evalue": 5.42e-30, "valid": false, "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_A0A920RY59" }, "pscc": { "uniref50_id": "UniRef50_A0A920RY59", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920RY59" ], "product": "ABC transporter domain-containing protein" }, "genes": [], "id": "KMADJIBOEG_141", "locus": "GCA000292445_00101" }, { "type": "cds", "sequence": "contig_1", "start": 50532, "stop": 50720, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTTTAAAACAATATCAGAAGAAAAAAACTGAGTTGAAAGCTGATATTAAAGCAACAAAGAACAAAGAACAAATCCAAAAATTAACTAAAGAACTTCAAACCTTAAAACAAAAATACGCTAATAAAAAAGCAATTGACAAAGAGTATGACAAATTAGTTGTAGCTTACAATAAGAAAGACTATTGA", "aa": "MRLKQYQKKKTELKADIKATKNKEQIQKLTKELQTLKQKYANKKAIDKEYDKLVVAYNKKDY", "aa_hexdigest": "a54fd7f1a739f1b43dedc21e7ba2c44f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7492.738500000004, "isoelectric_point": 9.885598182678223 }, "id": "KMADJIBOEG_142", "locus": "GCA000292445_00102" }, { "type": "cds", "sequence": "contig_1", "start": 50835, "stop": 51164, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00237D6FE8" ], "nt": "ATGGTTGGTTTAAAAGGTAAAGAAGATCGTTTGCCTGATGAATTATCAGGGGGAATGAAACAAAGAGTTGCTTTAGCACGTTCTTTAGTAGTAGAACCTGAAATTCTTTTATTAGATGAACCATTATCTGCACTTGATGCAAAGGTTAGAAAGAATTTACAAAAAGAATTACAACAAATTCATAAAAAAAGTGGATTGACTTTTATCTTAGTAACTCATGATCAAGAAGAGGCTTTAGTTTTATCAGATCGGATAGTTGTTATGAATGAGGGAAACATCTTACAAGTGGGTAATCCTGTTGACATTTATGACTCTCCTAAAACTGAATGA", "aa": "MVGLKGKEDRLPDELSGGMKQRVALARSLVVEPEILLLDEPLSALDAKVRKNLQKELQQIHKKSGLTFILVTHDQEEALVLSDRIVVMNEGNILQVGNPVDIYDSPKTE", "aa_hexdigest": "2ac62bb188addd48c6339b1c99c3a907", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00237D6FE8", "query_cov": 0.9908256880733946, "subject_cov": 0.8503937007874016, "identity": 0.574, "score": 124.0, "evalue": 1.36e-34, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI00237D6FE8" }, "pscc": { "uniref50_id": "UniRef50_UPI00237D6FE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00237D6FE8" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "KMADJIBOEG_143", "locus": "GCA000292445_00103" }, { "type": "cds", "sequence": "contig_1", "start": 51567, "stop": 51695, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAATCTTCTTTATTATTCCAATGACATGGATTATTGTTAGTGGATTACAAAGTGAAGATGGGGCTAGTATTAGTCAAAAATATGAACCACTTGTTAGTGGCTTAGGTTTTTTTAACAGTTTCTGA", "aa": "MTIFFIIPMTWIIVSGLQSEDGASISQKYEPLVSGLGFFNSF", "aa_hexdigest": "b037bcf4600047071b1bfb152a8cc3a7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4672.373599999999, "isoelectric_point": 4.136935234069824 }, "id": "KMADJIBOEG_144", "locus": "GCA000292445_00104" }, { "type": "cds", "sequence": "contig_1", "start": 51858, "stop": 52139, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAACCCTACTTGATTTATTAATTGGACTTTCTTTAAACAGAGTTGGTGATAACAACTTAACTTTTGGTTCAGGATATACCTTACTGGGAACAATTTATCTGTTTACTCCTTTTATGTTTTTACCACTTTATAACAACTTCTGTGTTTTACCTAAAAACTTGTTGTTAGCTAGTCAAGATTTGGGTTATAACTGGATTTACAGCTTTGTGAAAGTAGTAATTCCTTTTTCTAAAACAGCAATGTTATCAGGAATTGCTTTAATTTTTTCCCTGCTTTAA", "aa": "MKTLLDLLIGLSLNRVGDNNLTFGSGYTLLGTIYLFTPFMFLPLYNNFCVLPKNLLLASQDLGYNWIYSFVKVVIPFSKTAMLSGIALIFSLL", "aa_hexdigest": "030aa8acb8a3e22ab64ded1eca9711c2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10403.325299999991, "isoelectric_point": 8.851976585388183 }, "id": "KMADJIBOEG_145", "locus": "GCA000292445_00105" }, { "type": "cds", "sequence": "contig_1", "start": 52211, "stop": 52351, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTAATAATGGTTATGATAGTGCAATTGAAAGAGGCAGAGCTGCTGGAGCAATTATTATTGCTGCTTTAATTACTTTTGCAATTTACTTTACTGTTGTTTTTTTGCCTAAAATTGTCCGTATTGTTCATAACAAATGA", "aa": "MGNNGYDSAIERGRAAGAIIIAALITFAIYFTVVFLPKIVRIVHNK", "aa_hexdigest": "44c1359e8ab274375eb1da749145a0df", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5005.880099999999, "isoelectric_point": 9.987909507751464 }, "id": "KMADJIBOEG_146", "locus": "GCA000292445_00106" }, { "type": "cds", "sequence": "contig_1", "start": 52359, "stop": 52763, "strand": "+", "frame": 3, "gene": "potC", "product": "Spermidine/putrescine transport system permease PotC-like protein", "db_xrefs": [ "COG:COG1177", "COG:E", "GO:0005886", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P47290", "UniRef:UniRef90_P47290" ], "nt": "ATGAAAAAGCATTTTAAGAATTTAATTAAAAACAGTTATTTCTTTCTGTTAATAACTTTAATCTATTTACCACTTTTAATAGTTGTACTTGTTAGTTTAAACGGTTCTTCTTCAAGAGGAAATATAGTGCTTGATTTTGGTAATGTTTTAAATCCTAATCCTGATTCTAAATCTGCTTATTTAAGATTAGGTGAAACTGATTTTGCAACACCACTAATAAATTCAATCATTATAGGTGTGATCACTGTTTTAGTGTCTGTTCCTATTGCTGTTATCAGTGCGTTTGCGCTTTTAAGAACAAGGAATGCTTTAAAAAAGACAATCTTTGGAATTACTAATTTTTCTTTAGCAACTCCTGATATTATTACTGCTATCTCTTTAGTGTTGTTATTTGCTAACACTTGA", "aa": "MKKHFKNLIKNSYFFLLITLIYLPLLIVVLVSLNGSSSRGNIVLDFGNVLNPNPDSKSAYLRLGETDFATPLINSIIIGVITVLVSVPIAVISAFALLRTRNALKKTIFGITNFSLATPDIITAISLVLLFANT", "aa_hexdigest": "d5eb35154948182a746979f9cd434e24", "start_type": "ATG", "rbs_motif": null, "genes": [ "potC" ], "truncated": "3-prime", "pseudogene": { "start": 52359, "stop": 53066, "observations": { "mutation": [ 52761 ], "stop": [ 52761 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47290", "query_cov": 1.0, "subject_cov": 0.47183098591549294, "identity": 1.0, "score": 238.0, "evalue": 3.68e-77, "gene_start": 1, "gene_end": 134, "reference_start": 1, "reference_end": 134, "reference_sequence": "MKKHFKNLIKNSYFFLLITLIYLPLLIVVLVSLNGSSSRGNIVLDFGNVLNPNPDSKSAYLRLGETDFATPLINSIIIGVITVLVSVPIAVISAFALLRTRNALKKTIFGITNFSLATPDIITAISLVLLFANTWLSFNQQLGFFTIITSHISFSVPYALILIYPKIQKLNPNLILASQDLGYSPLKTFFHITLPYLMPSIFSAVLVVFATSFDDYVITSLVQGSVKTIATELYSFRKGIKAWAIAFGSILILISVLGVCLITLQKYLREKRKEIIKIRQWKNS" }, "paralog": false, "identity": 0.9957446808510638, "score": 445.0, "evalue": 3.83e-161, "description": "Internal stop codon at 52761. Nonsense mutation around 52761.", "psc": { "uniref90_id": "UniRef90_P47290", "gene": "potC", "product": "Spermidine/putrescine transport system permease protein PotC homolog", "uniref50_id": "UniRef50_P47290", "cog_id": "COG1177", "cog_category": "E", "go_ids": [ "GO:0005886", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47290", "UniRef:UniRef50_P47290", "COG:COG1177", "COG:E", "GO:0005886", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P47290", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47290" ], "product": "Spermidine/putrescine transport system permease protein PotC homolog" } }, "id": "KMADJIBOEG_147", "locus": "GCA000292445_00107" }, { "type": "cds", "sequence": "contig_1", "start": 52824, "stop": 53201, "strand": "+", "frame": 3, "gene": "potC", "product": "Spermidine/putrescine transport system permease PotC-like protein", "db_xrefs": [ "COG:COG1177", "COG:E", "GO:0005886", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P47290", "UniRef:UniRef90_P47290" ], "nt": "GTGCCTTATGCATTGATTTTGATTTACCCTAAAATTCAAAAATTGAATCCTAATTTAATTCTTGCTTCTCAAGATTTAGGCTATTCGCCTTTAAAAACTTTTTTCCATATTACTCTACCTTATCTAATGCCAAGTATTTTTTCAGCAGTACTAGTAGTATTTGCAACTAGTTTTGATGATTATGTAATTACCTCTTTAGTACAAGGATCAGTAAAAACTATAGCAACTGAACTCTATTCATTTAGAAAAGGAATTAAAGCATGGGCAATCGCCTTTGGGTCTATTCTCATATTGATTAGTGTCTTAGGAGTCTGTTTAATAACCCTGCAAAAGTATTTAAGGGAAAAAAGAAAGGAAATAATCAAAATAAGACAATGA", "aa": "MPYALILIYPKIQKLNPNLILASQDLGYSPLKTFFHITLPYLMPSIFSAVLVVFATSFDDYVITSLVQGSVKTIATELYSFRKGIKAWAIAFGSILILISVLGVCLITLQKYLREKRKEIIKIRQ", "aa_hexdigest": "e75e66aadada292443606bf52a3766c4", "start_type": "GTG", "rbs_motif": null, "genes": [ "potC" ], "truncated": "both", "pseudogene": { "start": 52524, "stop": 53213, "observations": { "mutation": [ 52761, 53199 ], "stop": [ 52761, 53199 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47290", "query_cov": 0.992, "subject_cov": 0.43661971830985913, "identity": 1.0, "score": 229.0, "evalue": 1.58e-73, "gene_start": 2, "gene_end": 125, "reference_start": 157, "reference_end": 280, "reference_sequence": "MKKHFKNLIKNSYFFLLITLIYLPLLIVVLVSLNGSSSRGNIVLDFGNVLNPNPDSKSAYLRLGETDFATPLINSIIIGVITVLVSVPIAVISAFALLRTRNALKKTIFGITNFSLATPDIITAISLVLLFANTWLSFNQQLGFFTIITSHISFSVPYALILIYPKIQKLNPNLILASQDLGYSPLKTFFHITLPYLMPSIFSAVLVVFATSFDDYVITSLVQGSVKTIATELYSFRKGIKAWAIAFGSILILISVLGVCLITLQKYLREKRKEIIKIRQWKNS" }, "paralog": false, "identity": 0.9912663755458515, "score": 427.0, "evalue": 3.83e-154, "description": "Internal stop codon at 52761, 53199. Nonsense mutation around 52761, 53199.", "psc": { "uniref90_id": "UniRef90_P47290", "gene": "potC", "product": "Spermidine/putrescine transport system permease protein PotC homolog", "uniref50_id": "UniRef50_P47290", "cog_id": "COG1177", "cog_category": "E", "go_ids": [ "GO:0005886", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47290", "UniRef:UniRef50_P47290", "COG:COG1177", "COG:E", "GO:0005886", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P47290", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47290" ], "product": "Spermidine/putrescine transport system permease protein PotC homolog" } }, "id": "KMADJIBOEG_148", "locus": "GCA000292445_00108" }, { "type": "cds", "sequence": "contig_1", "start": 53198, "stop": 53482, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACAGTTAAAATATTGCTTTTTCTCACTTTTTGTTAGTCTCTCATCAATATTGAGTAGTTGTGGTTCAACAACATTTGTACTAGCTAACTTTGAATCTTATATTTCGCCCTTATTGCTAGAAAGAGTACAAGAAAAACATCCCTTAACTTTCTTGACTTATCCTAGTAATGAAAAACTAATTAATGGTTTTGCTAACAACACTTATTCAGTAGCAGTAGCATCTACTTATGCAGTTAGTGAATTGATAGAAAGGGATCTATTATCACCAATAGATTGA", "aa": "MKKQLKYCFFSLFVSLSSILSSCGSTTFVLANFESYISPLLLERVQEKHPLTFLTYPSNEKLINGFANNTYSVAVASTYAVSELIERDLLSPID", "aa_hexdigest": "eac83ea179219eaf3c77ac5f95e39326", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10552.020999999988, "isoelectric_point": 5.723197746276856 }, "id": "KMADJIBOEG_149", "locus": "GCA000292445_00109" }, { "type": "cds", "sequence": "contig_1", "start": 53729, "stop": 54562, "strand": "+", "frame": 2, "gene": "potD", "product": "putative lipoprotein MG045", "db_xrefs": [ "COG:COG0687", "COG:E", "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75056", "UniRef:UniRef90_P47291" ], "nt": "GTGAAACACAAAGATCGCTTTAATGATAATAGGTTAGTTTTCATTGATGATGCTAGAACGATCTTTTCACTTGCTAACATCGTTAATACTAACAACAATTCAACTGATGTTAATCCAAAGGAAAATGGAATTGGTTATTTCACTAATGTCTATGAAAGCTTTCAAAGACTTGGATTAACAAAATCTAATTTAGATAGTATCTTTGTTAATTCTGATTCCAATATTGTGATCAATGAATTGGCAAGTGGTAGAAGACAAGGAGGAATTGTTTACAATGGTGATGCAGTGTATGCTGCATTGGGCGGTGATTTACGTGATGAATTGAGTGAAGAACAGATTCCTGATGGGAACAACTTTCACATTGTGCAACCCAAAATTTCCCCAGTTGCTTTAGATCTTTTGGTTATCAATAAACAACAACCTAATTTTCAAAAAGAAGCACATGAGATCATTTTTGATCTTGCTTTGGATGGTGCTGATCAAACTAAAGAACAGTTAATTAAAACTGATGAAGAATTGGGTACTGATGATGAAGACTTTTACTTAAAAGGAGCGATGCAAAACTTTAGTTATGTGAACTATGTTTCACCATTAAAAGTAATATCTGATCCAAGTACTGGAATAGTCAATTCCAAAAGGAATAATGCTGAAATGAAAAGTAAACAAATGTCAACTGATCAAATGACTAGTGAAAAAGAATTTGATTATTACACTGAAACACTTAAAGCATTATTAGAGAAAAAAGATAGTGAAGAATTAAATGAAAATGAAAAAAAACTAGTTGAAACCATTAAGAAAGCTTACACTATTGAAAAAGATAGTTCAATTCAGTGA", "aa": "MKHKDRFNDNRLVFIDDARTIFSLANIVNTNNNSTDVNPKENGIGYFTNVYESFQRLGLTKSNLDSIFVNSDSNIVINELASGRRQGGIVYNGDAVYAALGGDLRDELSEEQIPDGNNFHIVQPKISPVALDLLVINKQQPNFQKEAHEIIFDLALDGADQTKEQLIKTDEELGTDDEDFYLKGAMQNFSYVNYVSPLKVISDPSTGIVNSKRNNAEMKSKQMSTDQMTSEKEFDYYTETLKALLEKKDSEELNENEKKLVETIKKAYTIEKDSSIQ", "aa_hexdigest": "475b0630384d9f11f85c052260af0571", "start_type": "GTG", "rbs_motif": null, "genes": [ "potD" ], "truncated": "both", "pseudogene": { "start": 53429, "stop": 54646, "observations": { "mutation": [ 53480, 53621, 53705, 54560, 54620 ], "stop": [ 53480, 53621, 53705, 54560, 54620 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47291", "query_cov": 1.0, "subject_cov": 0.5734989648033126, "identity": 0.968, "score": 509.0, "evalue": 1.03e-178, "gene_start": 1, "gene_end": 277, "reference_start": 178, "reference_end": 454, "reference_sequence": "MKKQLKYCFFSLFVSLSSILSSCGSTTFVLANFESYISPLLLERVQEKHPLTFLTYPSNEKLINGFANNTYSVAVASTYAVSELIERDLLSPIDWSQFNLKKSSSSSDKVNNASDAKDLFIDSIKEISQQTKDSKNNELLHWAVPYFLQNLVFVYRGEKISELEQENVSWTDVIKAIVKHKDRFNDNRLVFIDDARTIFSLANIVNTNNNSADVNPKEDGIGYFTNVYESFQRLGLTKSNLDSIFVNSDSNIVINELASGRRQGGIVYNGDAVYAALGGDLRDELSEEQIPDGNNFHIVQPKISPVALDLLVINKQQSNFQKEAHEIIFDLALDGADQTKEQLIKTDEELGTDDEDFYLKGAMQNFSYVNYVSPLKVISDPSTGIVSSKKNNAEMKSKQMSTDQMTSEKEFDYYTETLKALLEKEDSAELNENEKKLVETIKKAYTIEKDSSIRWNQLVEKPISPLQRSNLSLSWLDFKLHWW" }, "paralog": false, "identity": 0.9679012345679012, "score": 758.0, "evalue": 2.68e-279, "description": "Internal stop codon at 53480, 53621, 53705, 54560, 54620. Nonsense mutation around 53480, 53621, 53705, 54560, 54620.", "psc": { "uniref90_id": "UniRef90_P47291", "gene": "PotD", "product": "Uncharacterized lipoprotein MG045", "uniref50_id": "UniRef50_P75056", "cog_id": "COG0687", "cog_category": "E", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47291", "UniRef:UniRef50_P75056", "COG:COG0687", "COG:E", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75056", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75056" ], "product": "Uncharacterized lipoprotein MG045 homolog" } }, "id": "KMADJIBOEG_150", "locus": "GCA000292445_00110" }, { "type": "cds", "sequence": "contig_1", "start": 54651, "stop": 55265, "strand": "+", "frame": 3, "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "db_xrefs": [ "COG:COG0533", "COG:J", "EC:2.3.1.234", "GO:0002949", "GO:0005506", "GO:0005737", "GO:0061711", "SO:0001217", "UniRef:UniRef50_P75055", "UniRef:UniRef90_P47292" ], "nt": "ATGGAACAGCCGTTGTGTGTTTTAGGGATTGAAACAACCTGTGATGATACAGGTCTTAGTATTGTCATTGATCAAAAAATCAAGAGTAACATTGTTATCTCTTCTGCTAACTTACATGTAAAAACAGGAGGAGTTGTACCTGAAATTGCAGCACGATGCCACGAACAAAATCTCTTTAAAGCAATAAGAGATTTAAATTTTGAGATAAGAGATTTATCTCACATTGCTTATGCATGTAATCCTGGGTTAGCAGGATGTTTACATGTGGGAGCCACTTTTGCTAGAAGCTTAAGTTTCTTATTAGACAAACCATTGTTACCCATCAACCATCTTTATGCGCATATCTTTTCTTGTTTAATTGATCAAGATTTAAATAAGCTGCAATTACCAGCATTAGGCCTTGTAATTTCAGGTGGTCATACTGCTATTTATCTAGTTAAATCATTTTATGAACTTGAACTAATTGCTGAAACTAGTGATGATGCAATTGGTGAAGTTTATGACAAGATAGGCAGAGCAATGGGCTTTGATTATCCTGCTGGTAGTAAAATTGATAGTCTTTTTAATAAAGAATTAGTTAAACCTCACTATTTCTTTAAACCTTCTACTAAGTGA", "aa": "MEQPLCVLGIETTCDDTGLSIVIDQKIKSNIVISSANLHVKTGGVVPEIAARCHEQNLFKAIRDLNFEIRDLSHIAYACNPGLAGCLHVGATFARSLSFLLDKPLLPINHLYAHIFSCLIDQDLNKLQLPALGLVISGGHTAIYLVKSFYELELIAETSDDAIGEVYDKIGRAMGFDYPAGSKIDSLFNKELVKPHYFFKPSTK", "aa_hexdigest": "276fba995d1708c809dbb1bf246e6bd0", "start_type": "ATG", "rbs_motif": null, "genes": [ "tsaD" ], "truncated": "3-prime", "pseudogene": { "start": 54651, "stop": 55568, "observations": { "mutation": [ 55263 ], "stop": [ 55263 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47292", "query_cov": 1.0, "subject_cov": 0.6476190476190476, "identity": 1.0, "score": 416.0, "evalue": 1.41e-145, "gene_start": 1, "gene_end": 204, "reference_start": 1, "reference_end": 204, "reference_sequence": "MEQPLCVLGIETTCDDTGLSIVIDQKIKSNIVISSANLHVKTGGVVPEIAARCHEQNLFKAIRDLNFEIRDLSHIAYACNPGLAGCLHVGATFARSLSFLLDKPLLPINHLYAHIFSCLIDQDLNKLQLPALGLVISGGHTAIYLVKSFYELELIAETSDDAIGEVYDKIGRAMGFDYPAGSKIDSLFNKELVKPHYFFKPSTKWTKFSYSGLKSQCLNKIKQISANKTRIDWSELASNFQATIIDHYIDHVKNAIKKFAPKMLLVGGGVSANSYLSNRISTLNLPFLIADSKYTSDNGAMIGFYASLLINGDKN" }, "paralog": false, "identity": 0.9967213114754099, "score": 608.0, "evalue": 1.02e-223, "description": "Internal stop codon at 55263. Nonsense mutation around 55263.", "psc": { "uniref90_id": "UniRef90_P47292", "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_P75055", "cog_id": "COG0533", "cog_category": "J", "go_ids": [ "GO:0002949", "GO:0005506", "GO:0005737", "GO:0061711" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47292", "EC:2.3.1.234", "UniRef:UniRef50_P75055", "COG:COG0533", "COG:J", "GO:0002949", "GO:0005506", "GO:0005737", "GO:0061711" ] }, "pscc": { "uniref50_id": "UniRef50_P75055", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75055" ], "product": "tRNA N6-adenosine threonylcarbamoyltransferase" } }, "id": "KMADJIBOEG_151", "locus": "GCA000292445_00111" }, { "type": "cds", "sequence": "contig_1", "start": 55437, "stop": 55598, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGTTAGTAGGAGGTGGAGTTAGTGCCAATTCTTATCTATCTAACAGAATTAGTACATTAAATTTACCCTTTTTAATTGCTGATAGCAAATACACCAGTGATAATGGAGCAATGATTGGTTTTTATGCATCACTTTTAATTAATGGCGATAAGAATTAA", "aa": "MLLVGGGVSANSYLSNRISTLNLPFLIADSKYTSDNGAMIGFYASLLINGDKN", "aa_hexdigest": "6946ae4a63279b6a7e7fea716897fbf0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5598.319300000001, "isoelectric_point": 5.793621253967285 }, "id": "KMADJIBOEG_152", "locus": "GCA000292445_00112" }, { "type": "cds", "sequence": "contig_1", "start": 55582, "stop": 55812, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00438.25" ], "nt": "ATGGCGATAAGAATTAAAAGTACAAGAGTTGGTAGATTTGTTTCTGAATCAGTGGGATTAGGTCATCCTGATAAAATTTGTGATCAGATTGCAGATAGTATCTTAGACCAATGTTTACTACAGAGTAAAATTAGTCATGTAGCATGTGAAGTCTTTGCTTCTAAAAACCTTATTTTAATAGGTGGTGAGATTTCAACAAGTGGCTATGTTGATGTTGTTCAAACTGCTTGA", "aa": "MAIRIKSTRVGRFVSESVGLGHPDKICDQIADSILDQCLLQSKISHVACEVFASKNLILIGGEISTSGYVDVVQTA", "aa_hexdigest": "b4ebd3ac6f81b4a62c8c383f05bb943a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00438.25", "name": "S-AdoMet_synt_N", "length": 63, "aa_cov": 0.8289473684210527, "hmm_cov": 1.0, "evalue": 1.495456867753605e-25, "score": 84.82149505615234, "start": 13, "stop": 75 } ], "seq_stats": { "molecular_weight": 8133.315999999996, "isoelectric_point": 6.01432704925537 }, "id": "KMADJIBOEG_153", "locus": "GCA000292445_00113" }, { "type": "cds", "sequence": "contig_1", "start": 55957, "stop": 56472, "strand": "+", "frame": 1, "gene": "metK", "product": "methionine adenosyltransferase", "db_xrefs": [ "COG:COG0192", "COG:H", "EC:2.5.1.6", "GO:0000287", "GO:0004478", "GO:0005524", "GO:0005829", "GO:0006556", "GO:0006730", "KEGG:K00789", "SO:0001217", "UniRef:UniRef50_P47293", "UniRef:UniRef90_P47293" ], "nt": "GTGAATGAAACAAAGCAACTAATGCCTTTAGGAGTTTTACTAGCACACTCGTTTTTAAAACAAGCAGAAAAACTAACAAAACAATTTGATTTTTTAAAAAATGATATGAAAAGTCAAGTGGTTTTAAACTACAGTTTAAACCAAGTTGAATGTGAAGAAGTTTTACTATCAATTCAACACACTAATGCTATTAGTTTAACAGAATTGAGAAAAGTGATTGAAAATAATGTAATTCTACCTGTTTTAAACCAATATGGTTTTCAAGATAAAAAGCCAACTTGTTTAGTGAATCCTGGTGGTTCTTTTGTTTTAGGTGGACCTATGGCAGATACTGGATTAACTGGTAGAAAAATCATTGTTGACACCTATGGTCCATATGCTCACCATGGTGGTGGTAGCTTTAGCGGAAAAGATCCTAGTAAGGTGGATAGAACAGGTGCTTATTTTGCACGTTTTATCGCAAAACATATTGTAAGTTTAGGCTGGGCCAGTGAGTGTGAAGTCAGTATTAGCTGA", "aa": "MNETKQLMPLGVLLAHSFLKQAEKLTKQFDFLKNDMKSQVVLNYSLNQVECEEVLLSIQHTNAISLTELRKVIENNVILPVLNQYGFQDKKPTCLVNPGGSFVLGGPMADTGLTGRKIIVDTYGPYAHHGGGSFSGKDPSKVDRTGAYFARFIAKHIVSLGWASECEVSIS", "aa_hexdigest": "7cbf5654cc2b87a4fbb0719aa3d9d84b", "start_type": "GTG", "rbs_motif": null, "genes": [ "metK" ], "truncated": "both", "pseudogene": { "start": 55657, "stop": 56733, "observations": { "mutation": [ 55810, 56470, 56578, 56677 ], "stop": [ 55810, 56470, 56578, 56677 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47293", "query_cov": 0.9941520467836257, "subject_cov": 0.44386422976501305, "identity": 1.0, "score": 343.0, "evalue": 2.92e-116, "gene_start": 2, "gene_end": 171, "reference_start": 127, "reference_end": 296, "reference_sequence": "MAIRIKSTRVGRFVSESVGLGHPDKICDQIADSILDQCLLQSKTSHVACEVFASKNLILIGGEISTSGYVDVVQTAWRILRNLGYNETDFSFLSCINNQSLEINQAVLKNNEINAGDQGITVGYAVNETKQLMPLGVLLAHSFLKQAEKLTKQFDFLKNDMKSQVVLNYSLNQVECEEVLLSIQHTNAISLTELRKVIENNVILPVLNQYGFQDKKPTCLVNPGGSFVLGGPMADTGLTGRKIIVDTYGPYAHHGGGSFSGKDPSKVDRTGAYFARFIAKHIVSLGWASECEVSISWVFSKPNPQSITVKCFNTNIQYDEVLINRVVNNYFNWSITKIIDKLKLLDFVKYSDYAVYGHFGNDLSPWEQPTELDKLECLIKNFH" }, "paralog": false, "identity": 0.9804469273743017, "score": 697.0, "evalue": 2.23e-258, "description": "Internal stop codon at 55810, 56470, 56578, 56677. Nonsense mutation around 55810, 56470, 56578, 56677.", "psc": { "uniref90_id": "UniRef90_P47293", "gene": "metK", "product": "methionine adenosyltransferase", "ec_ids": [ "2.5.1.6" ], "uniref50_id": "UniRef50_P47293", "cog_id": "COG0192", "cog_category": "H", "kegg_orthology_id": "K00789", "go_ids": [ "GO:0000287", "GO:0004478", "GO:0005524", "GO:0005829", "GO:0006556", "GO:0006730" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47293", "EC:2.5.1.6", "UniRef:UniRef50_P47293", "COG:COG0192", "COG:H", "KEGG:K00789", "GO:0000287", "GO:0004478", "GO:0005524", "GO:0005829", "GO:0006556", "GO:0006730" ] }, "pscc": { "uniref50_id": "UniRef50_P47293", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47293" ], "product": "S-adenosylmethionine synthase" } }, "id": "KMADJIBOEG_154", "locus": "GCA000292445_00114" }, { "type": "cds", "sequence": "contig_1", "start": 56709, "stop": 56888, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAATCAAAAATTTCCATTAGATCCTAATAAAAAAAAGGAACAACAAGGCGTAGTAAAACCAAATCTACCGGTTGTTAAAGAAAAGAAAAACAGCCATTTAAAAAACCAAATTGGAGTGAATTTAAGCTGTTCAACTTTTTTAAAAAGCACACTTATTTTTGCTTGGTTGTATTAG", "aa": "MLNQKFPLDPNKKKEQQGVVKPNLPVVKEKKNSHLKNQIGVNLSCSTFLKSTLIFAWLY", "aa_hexdigest": "ac6444badcf761549885974cd440e27f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6783.958099999998, "isoelectric_point": 9.937495231628418 }, "id": "KMADJIBOEG_155", "locus": "GCA000292445_00115" }, { "type": "cds", "sequence": "contig_1", "start": 57049, "stop": 57168, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02978.25" ], "nt": "ATGACTAGAGAAGAAAGGAGACATCCCAAATTAATTAATCGTGATCCTAATAGAAAACAGCGCATCATAAAAGGATCAGGGAGAAAAATGGATGAGTTAAACAAACTGATGAAGGAATGA", "aa": "MTREERRHPKLINRDPNRKQRIIKGSGRKMDELNKLMKE", "aa_hexdigest": "ad263c070bd32515b7247a288903f725", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02978.25", "name": "SRP_SPB", "length": 39, "aa_cov": 1.0, "hmm_cov": 0.9487179487179487, "evalue": 8.20248719780357e-12, "score": 40.56576919555664, "start": 1, "stop": 39 } ], "seq_stats": { "molecular_weight": 4803.603900000001, "isoelectric_point": 10.963251686096193 }, "id": "KMADJIBOEG_156", "locus": "GCA000292445_00116" }, { "type": "cds", "sequence": "contig_1", "start": 57187, "stop": 57909, "strand": "-", "frame": 1, "gene": "ffh", "product": "signal recognition particle protein", "db_xrefs": [ "COG:COG0541", "COG:U", "EC:3.6.5.4", "GO:0003924", "GO:0005048", "GO:0005525", "GO:0006614", "GO:0008312", "GO:0009306", "GO:0016887", "GO:0048500", "GO:0048501", "KEGG:K03106", "SO:0001217", "UniRef:UniRef50_P75054", "UniRef:UniRef90_P47294" ], "nt": "ATGTTAGTAGGCTTGGACATCTACAGACCCGCTGCCATTGAACAACTTGAAACGCTTTCACAACAAACTAACAGCGTATTTTTTGCACAAGGCACTCAACCAGTTGCTAAAACAACAAAAGCAGCACTCAGTGCTTTTAAAACTGCAAAATGTCAAGCAATCATTTGTGATACCGCTGGTAGATTACAAACAAATGAAACATTAATGGATGAATTGGTAAGTGTTAAAAATGAATTAAATCCTGATGAAATTATCATGGTAGTAGATGGATTAAGCGGTCAGGAAATTATCAATGTTGCTCAAACGTTCCACAAACGTTTAAAACTAACTGGATTTATTATCAGTAAATTAGACAGTGATGCTAGAGCAGGAGCTGCACTTTCATTAGCTTCACTTTTACAAGTACCCATTAAATTAATTGGTGTTTCTGAAAAATTAGATGGATTGGAACAATTTCATCCTGAAAGGATAGCCAATCGGATCTTAGGTTTGGGTGATGTAATGAGTTTAGTTGAAAAAGCTGAACAAGTTTTTGATAAAAAAGATTTAACTAAAACCATCAGCAAGATGTTTTTGGGAAAAATGGATTTAGAAGATCTTTTGATCTACATGCAACAAATGCACAAAATGGGAAGTGTCAGTTCACTGATAAAAATGTTGCCTGCTAACTTTTCTGTATCAGAAGAAAATGCTGAATTAATTGAAAACAAAATTGAACTATGA", "aa": "MLVGLDIYRPAAIEQLETLSQQTNSVFFAQGTQPVAKTTKAALSAFKTAKCQAIICDTAGRLQTNETLMDELVSVKNELNPDEIIMVVDGLSGQEIINVAQTFHKRLKLTGFIISKLDSDARAGAALSLASLLQVPIKLIGVSEKLDGLEQFHPERIANRILGLGDVMSLVEKAEQVFDKKDLTKTISKMFLGKMDLEDLLIYMQQMHKMGSVSSLIKMLPANFSVSEENAELIENKIEL", "aa_hexdigest": "3b190a50b43f95df42a1b232bfb5d714", "start_type": "ATG", "rbs_motif": null, "genes": [ "ffh" ], "truncated": "both", "pseudogene": { "start": 56962, "stop": 58209, "observations": { "mutation": [ 57051, 57189, 57939 ], "stop": [ 57051, 57189, 57939 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47294", "query_cov": 1.0, "subject_cov": 0.5381165919282511, "identity": 0.996, "score": 414.0, "evalue": 4.44e-142, "gene_start": 1, "gene_end": 240, "reference_start": 132, "reference_end": 371, "reference_sequence": "MFKAMLSSIVMRTMQKKINAQTITEKDVELVLKEIRIALLDADVNLLVVKNFIKAIRDKTVGQTIEPGQDLQKSLLKTIKTELINILSQPNQELNEKRPLKIMMVGLQGSGKTTTCGKLAYWLEKKYKQKTMLVGLDIYRPAAIEQLETLSQQTNSVFFAQGTQPVAKTTKAALSAFKTAKCQTIICDTAGRLQTNETLMDELVSVKNELNPDEIIMVVDGLSGQEIINVAQTFHKRLKLTGFIISKLDSDARAGAALSLASLLQVPIKLIGVSEKLDGLEQFHPERIANRILGLGDVMSLVEKAEQVFDKKDLTKTISKMFLGKMDLEDLLIYMQQMHKMGSVSSLIKMLPANFSVSEENAELIENKIELWKVLINSMTREERRHPKLINRDPNRKQRIIKGSGRKMDELNKLMKEWNKMQLKATEMGKLLKTGSNPFGGFGQFF" }, "paralog": false, "identity": 0.9903614457831326, "score": 750.0, "evalue": 2.47e-277, "description": "Internal stop codon at 57051, 57189, 57939. Nonsense mutation around 57051, 57189, 57939.", "psc": { "uniref90_id": "UniRef90_P47294", "gene": "ffh", "product": "signal recognition particle protein", "ec_ids": [ "3.6.5.4" ], "uniref50_id": "UniRef50_P75054", "cog_id": "COG0541", "cog_category": "U", "kegg_orthology_id": "K03106", "go_ids": [ "GO:0003924", "GO:0005048", "GO:0005525", "GO:0006614", "GO:0008312", "GO:0009306", "GO:0016887", "GO:0048500", "GO:0048501" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47294", "EC:3.6.5.4", "UniRef:UniRef50_P75054", "COG:COG0541", "COG:U", "KEGG:K03106", "GO:0003924", "GO:0005048", "GO:0005525", "GO:0006614", "GO:0008312", "GO:0009306", "GO:0016887", "GO:0048500", "GO:0048501" ] }, "pscc": { "uniref50_id": "UniRef50_P75054", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75054" ], "product": "Signal recognition particle protein" } }, "id": "KMADJIBOEG_157", "locus": "GCA000292445_00117" }, { "type": "cds", "sequence": "contig_1", "start": 57937, "stop": 58272, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00448.28", "PFAM:PF02881.24" ], "nt": "ATGCGCACGATGCAAAAAAAAATTAACGCTCAAACGATCACTGAGAAAGATGTAGAGTTAGTTCTAAAAGAGATTAGAATTGCATTGCTTGATGCTGATGTTAACCTGCTTGTTGTTAAAAATTTCATCAAAGCAATTAGAGATAAAACAGTAGGACAAACCATTGAACCTGGTCAAGATTTGCAAAAGTCTCTATTAAAAACAATCAAAACAGAACTAATTAATATCTTAAGCCAACCCAACCAAGAACTAAATGAAAAAAGACCTTTAAAAATAATGATGGTTGGTTTACAAGGATCAGGTAAAACAACAACTTGTGGCAAACTAGCTTATTGA", "aa": "MRTMQKKINAQTITEKDVELVLKEIRIALLDADVNLLVVKNFIKAIRDKTVGQTIEPGQDLQKSLLKTIKTELINILSQPNQELNEKRPLKIMMVGLQGSGKTTTCGKLAY", "aa_hexdigest": "c5de0bd3bf8dd932b62cf6bb17c685b0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00448.28", "name": "SRP54", "length": 20, "aa_cov": 0.18018018018018017, "hmm_cov": 1.0, "evalue": 1.5084301463435073e-08, "score": 29.639175415039062, "start": 91, "stop": 110 }, { "id": "PF02881.24", "name": "SRP54_N", "length": 58, "aa_cov": 0.5135135135135135, "hmm_cov": 1.0, "evalue": 1.1035632057210709e-15, "score": 52.79533767700195, "start": 9, "stop": 65 } ], "seq_stats": { "molecular_weight": 12461.65679999999, "isoelectric_point": 9.58872127532959 }, "id": "KMADJIBOEG_158", "locus": "GCA000292445_00118" }, { "type": "cds", "sequence": "contig_1", "start": 58277, "stop": 58684, "strand": "+", "frame": 2, "gene": null, "product": "Uridine phosphorylase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9D1M2H1" ], "nt": "ATGCTTGATAACATTGCTTTGAACATGATTTTTAATTATTTATTATTAAATAATGTTTTAATAAAACAATATTGCAATATGACCCCACATATAAGTGCTAAGAAAGATGACATTAGCAAAGTTGTTTTAATGCCAGGTGATCCATTGAGAGCTAAATGGATAGCTGAGCAATTCTTAGATCAAGCTAAATTAGTCAATGAAGTGAGGGGAATGTTTGCTTATACTGGGCAGTATAAATCTAAAACAGTTACAGTAATGGGCCATGGAATGGGGATCCCTTCTATTGGAATTTATTCATATGAGTTGATGAATTTTTATGAGGTTGAAACTATCATTAGAATCGGAAGTTGTGGTGCTTTAGCACCGCAATTAAAATTAAAAGATCTTGTTATTGCTTCAAAAGCATGA", "aa": "MLDNIALNMIFNYLLLNNVLIKQYCNMTPHISAKKDDISKVVLMPGDPLRAKWIAEQFLDQAKLVNEVRGMFAYTGQYKSKTVTVMGHGMGIPSIGIYSYELMNFYEVETIIRIGSCGALAPQLKLKDLVIASKA", "aa_hexdigest": "5e6b0e9cbc242a2599f07e5f94feb7ac", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001FEB9C95", "query_cov": 0.8074074074074075, "subject_cov": 0.8449612403100775, "identity": 0.624, "score": 143.0, "evalue": 1.4e-41, "valid": false, "uniref50_id": "UniRef50_A0A9D1M2H1" }, "pscc": { "uniref50_id": "UniRef50_A0A9D1M2H1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9D1M2H1" ], "product": "Uridine phosphorylase" }, "genes": [], "id": "KMADJIBOEG_159", "locus": "GCA000292445_00119" }, { "type": "cds", "sequence": "contig_1", "start": 58709, "stop": 59071, "strand": "+", "frame": 2, "gene": "deoD", "product": "purine-nucleoside phosphorylase", "db_xrefs": [ "COG:COG0813", "COG:F", "EC:2.4.2.1", "GO:0004731", "GO:0005829", "GO:0006139", "GO:0006152", "KEGG:K03784", "SO:0001217", "UniRef:UniRef50_P47295", "UniRef:UniRef90_P47295" ], "nt": "ATGGGTGTTGAAATTCCAGAAGATAAGATCTTATTTGCAACAAGTTCTTTAGTGGAATTAGCAAAAGAAACTGCGATTAAGAACAAGCTTGATTTTCATGAAGGATTAGTATTTTGTGAGGATGCTTTTTATCAAACTAGAAAAGATGTAATTAGTCTTGCTAAAGAAAAAAATAGTTTAGCAGTTGAAATGGAAGCACATGCACTTTATGCTAATGCAATCCTGTTGAAGAAAAAAGCACTTACACTCTTAACAGTATCTGATTCTCTAGTAACTCATGAAGCACTTAGTTCTGAATTAAGACAAAAGTCATTTAAGCAAATGGCTTTATTAGCACTTGAAATGACTCAAAAACTAATCTAA", "aa": "MGVEIPEDKILFATSSLVELAKETAIKNKLDFHEGLVFCEDAFYQTRKDVISLAKEKNSLAVEMEAHALYANAILLKKKALTLLTVSDSLVTHEALSSELRQKSFKQMALLALEMTQKLI", "aa_hexdigest": "b5531ca3f8d05936b458016ffb9425be", "start_type": "ATG", "rbs_motif": null, "genes": [ "deoD" ], "truncated": "5-prime", "pseudogene": { "start": 58409, "stop": 59071, "observations": { "mutation": [ 58682 ], "stop": [ 58682 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47295", "query_cov": 1.0, "subject_cov": 0.5042016806722689, "identity": 1.0, "score": 221.0, "evalue": 2.33e-71, "gene_start": 1, "gene_end": 120, "reference_start": 119, "reference_end": 238, "reference_sequence": "MTPHISAKKDDISKVVLMPGDPLRAKWIAEQFLDQAKLVNEVRGMFAYTGQYKSKTVTVMGHGMGIPSIGIYSYELMNFYEVETIIRIGSCGALAPQLKLKDLVIASKAWSESIYAKDMGVEIPEDKILFATSSLVELAKETAIKNKLDFHEGLVFCEDAFYQTRKDVISLAKEKNSLAVEMEAHALYANAILLKKKALTLLTVSDSLVTHEALSSELRQKSFKQMALLALEMTQKLI" }, "paralog": false, "identity": 0.9954545454545455, "score": 421.0, "evalue": 2.35e-152, "description": "Internal stop codon at 58682. Nonsense mutation around 58682.", "psc": { "uniref90_id": "UniRef90_P47295", "gene": "deoD", "product": "purine-nucleoside phosphorylase", "ec_ids": [ "2.4.2.1" ], "uniref50_id": "UniRef50_P47295", "cog_id": "COG0813", "cog_category": "F", "kegg_orthology_id": "K03784", "go_ids": [ "GO:0004731", "GO:0005829", "GO:0006139", "GO:0006152" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47295", "EC:2.4.2.1", "UniRef:UniRef50_P47295", "COG:COG0813", "COG:F", "KEGG:K03784", "GO:0004731", "GO:0005829", "GO:0006139", "GO:0006152" ] }, "pscc": { "uniref50_id": "UniRef50_P47295", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47295" ], "product": "Purine nucleoside phosphorylase DeoD-type" } }, "id": "KMADJIBOEG_160", "locus": "GCA000292445_00120" }, { "type": "cds", "sequence": "contig_1", "start": 59075, "stop": 59746, "strand": "+", "frame": 2, "gene": "deoC", "product": "deoxyribose-phosphate aldolase", "db_xrefs": [ "COG:COG0274", "COG:F", "EC:4.1.2.4", "GO:0004139", "GO:0005737", "GO:0006018", "GO:0009264", "GO:0016052", "KEGG:K01619", "RefSeq:WP_014894119.1", "SO:0001217", "UniParc:UPI00027B3648", "UniRef:UniRef100_UPI00027B3648", "UniRef:UniRef50_P09924", "UniRef:UniRef90_P47296" ], "nt": "ATGAAATTAGAATACAACCGGATTATTGATAGCACCTTAGTCAAAGCTGATACGCTTCCCCATGAAATAGATACTTTATGTGCTGATGCTCATAAATACCAGTTTTTTGCAGTATGTGTTAATCCTAGTTATGTTAGTTGTGCTAAAAATATCTTGAAAAATACTGCAGTTCAACTCTGTTGTGTTGTTGGTTTTCCCTTAGGACAAACAACCCAAAAACAGAAGGTATATGAAGCTAAGATTGCTATTAAAGAGGGAGCGGATGAAATTGATATGGTAATGAATATTGCTGAGTTTAAAAAACGTTGTGCTTGTGTTATTACTGAAATTAGAGCTGTTAAAAAAGTGTGTGGCAAGCGTAAATTAAAAGTAATTATTGAAACTGCACTTTTAACAAATGATGAAATCAAAGATGCAGTTAATGTTTGCATTGATGGCAATGCAGATTATGTTAAAACTTCCACTGGTTTTTCTTTCCGTGGTGCATCTTTAGAAGATGTTCAGATTATGAATAATGCTGCAGCAAATTTAATTAAAATCAAAGCTTCAGGTGGGATTAAAACAGCAAAGCAATTTATAGATTTATTTCAAGCTGGAGCTAGTAGAATTGGAACTTCAAATGCGGTCCAAATAATGCAAGAATTAAACAAAATGAACCATGAATATCATTAA", "aa": "MKLEYNRIIDSTLVKADTLPHEIDTLCADAHKYQFFAVCVNPSYVSCAKNILKNTAVQLCCVVGFPLGQTTQKQKVYEAKIAIKEGADEIDMVMNIAEFKKRCACVITEIRAVKKVCGKRKLKVIIETALLTNDEIKDAVNVCIDGNADYVKTSTGFSFRGASLEDVQIMNNAAANLIKIKASGGIKTAKQFIDLFQAGASRIGTSNAVQIMQELNKMNHEYH", "aa_hexdigest": "10b4d41c3c328c3b73d8327e5cedd361", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3648", "ncbi_nrp_id": "WP_014894119.1", "uniref100_id": "UniRef100_UPI00027B3648", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3648", "RefSeq:WP_014894119.1", "UniRef:UniRef100_UPI00027B3648" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3648", "uniref90_id": "UniRef90_P47296" }, "psc": { "uniref90_id": "UniRef90_P47296", "gene": "deoC", "product": "deoxyribose-phosphate aldolase", "ec_ids": [ "4.1.2.4" ], "uniref50_id": "UniRef50_P09924", "cog_id": "COG0274", "cog_category": "F", "kegg_orthology_id": "K01619", "go_ids": [ "GO:0004139", "GO:0005737", "GO:0006018", "GO:0009264", "GO:0016052" ] }, "pscc": { "uniref50_id": "UniRef50_P09924", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P09924" ], "product": "Deoxyribose-phosphate aldolase" }, "genes": [ "deoC" ], "id": "KMADJIBOEG_161", "locus": "GCA000292445_00121" }, { "type": "cds", "sequence": "contig_1", "start": 59733, "stop": 60656, "strand": "+", "frame": 3, "gene": null, "product": "Pyrimidine-nucleoside phosphorylase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A354QR12" ], "nt": "ATGAATATCATTAATTTAATTAATAAAAAACAACGCGGAAAAGCTTTAAATTTAGCTGAAATCAATTGGTTTGTTAATGCTGTTTTAAACAAAACCATTGCTGATTATCAAATTACTGCATTTTTGATGGCTATTTGGTTTAAAGGGATGAACCCAAATGAACTTTTTTTATTAACAAAAGCAATGGTGGATACTGGTGAAATTATTAAGTTTAATCACCATGGCAAGATTAGTGTTGATAAACATTCAACTGGTGGTATTGGTGATAAGGTTTCTTTAGCATTGGTTCCTATCTTAACTAGTTTAGGATTTAGTGTTGCTAAATTATCAGGAAGAGGCCTTGGTTATACTGGTGGAACAATTGATAAATTAGAAGCAGTTGGAGTTAAAACAGAATTAACTGACCAACAAGCACAAGCATGTTTAGATAAAAATGATTGTTTTATCATCGGACAAAGTAAGGACATTGCACCAGTTGATAAAGTACTTTATGGTTTAAGAGATATTACTGGAACAGTTGATAGTTTGCCTTTAATTGCATCTAGTATTATGTCTAAAAAGCTAGCAGTTATGAACGAGTATATTTTCATTGATCTTAAATATGGAAAAGGTGCCTTTTGTAAAACTAAGAAAATTGCTAACGAACTTGCAAAACTGATGCAAAGTATTGCTAAAAGTTTTAAAAGAAAGCTGTCTGTTAAATTAAGTGATATGAATCAAGTACTTGGTAAAGCTGTTGGTAACGTAATTGAAGTTAATGAAGCTGTTAACTTTCTAAAACAAGATTTAGATCAAGTAGGACAAGATTTTATTGATTTAATGCAAGCAATTGTTATTAACATTCTACTTGAAACAAAACAAGCAAAAACCAAACAAAAGGCTATTGAACTTTATCAGGATGTTTTAACTAGTAAAAAAGCATGA", "aa": "MNIINLINKKQRGKALNLAEINWFVNAVLNKTIADYQITAFLMAIWFKGMNPNELFLLTKAMVDTGEIIKFNHHGKISVDKHSTGGIGDKVSLALVPILTSLGFSVAKLSGRGLGYTGGTIDKLEAVGVKTELTDQQAQACLDKNDCFIIGQSKDIAPVDKVLYGLRDITGTVDSLPLIASSIMSKKLAVMNEYIFIDLKYGKGAFCKTKKIANELAKLMQSIAKSFKRKLSVKLSDMNQVLGKAVGNVIEVNEAVNFLKQDLDQVGQDFIDLMQAIVINILLETKQAKTKQKAIELYQDVLTSKKA", "aa_hexdigest": "8dfeec9dc9ab042e4c27adedf2ac68c4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000A66C754", "query_cov": 0.8045602605863192, "subject_cov": 1.0, "identity": 0.514, "score": 236.0, "evalue": 5.88e-74, "valid": false, "product": "thymidine phosphorylase", "ec_ids": [ "2.4.2.4" ], "uniref50_id": "UniRef50_A0A354QR12", "go_ids": [ "GO:0006206" ] }, "pscc": { "uniref50_id": "UniRef50_A0A354QR12", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A354QR12" ], "product": "Pyrimidine-nucleoside phosphorylase" }, "genes": [], "id": "KMADJIBOEG_162", "locus": "GCA000292445_00122" }, { "type": "cds", "sequence": "contig_1", "start": 60861, "stop": 60998, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGGGTTATTTTTAGTGAAAAAAGATAATGAGTCTGTGGCAGTTGGAGATACTGTATTAAACCTGTATAGTTCTAATCCTATTAGCAATGAAGATATCTCTGCTGCTCAAAAAACAATAATTATTAATAAATAA", "aa": "MAGLFLVKKDNESVAVGDTVLNLYSSNPISNEDISAAQKTIIINK", "aa_hexdigest": "bbeb09d191d4c139a4fa417a6baba990", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4822.446000000001, "isoelectric_point": 4.8610639572143555 }, "id": "KMADJIBOEG_163", "locus": "GCA000292445_00123" }, { "type": "cds", "sequence": "contig_1", "start": 61007, "stop": 61399, "strand": "+", "frame": 2, "gene": "cdd", "product": "cytidine deaminase", "db_xrefs": [ "COG:COG0295", "COG:F", "EC:3.5.4.5", "GO:0004126", "GO:0005737", "GO:0005829", "GO:0008270", "GO:0008655", "GO:0009972", "GO:0042802", "KEGG:K01489", "RefSeq:WP_010869308.1", "SO:0001217", "UniParc:UPI0000127407", "UniRef:UniRef100_P47298", "UniRef:UniRef50_P75051", "UniRef:UniRef90_P47298" ], "nt": "ATGAAGGTGAATTTAGAGTGGATAATTAAACAGTTACAAATGATAGTTAAAAGAGCATATACTCCCTTTTCTAACTTTAAAGTTGCATGTATGATTATTGCTAACAACCAAACTTTTTTTGGAGTTAACATTGAAAATTCTTCCTTTCCAGTAACTTTGTGTGCTGAAAGAAGCGCCATTGCTAGCATGGTTACAAGTGGTCATAGGAAAATTGATTATGTTTTTGTTTACTTCAATACTAAAAATAAGAGTAACTCACCCTGTGGAATGTGCAGACAAAACTTACTGGAATTTTCCCATCAAAAAACAAAGCTTTTTTGTATTGATAATGATAGTAGTTATAAACAATTTTCCATTGATGAATTATTAATGAATGGTTTTAAAAAGAGCTAA", "aa": "MKVNLEWIIKQLQMIVKRAYTPFSNFKVACMIIANNQTFFGVNIENSSFPVTLCAERSAIASMVTSGHRKIDYVFVYFNTKNKSNSPCGMCRQNLLEFSHQKTKLFCIDNDSSYKQFSIDELLMNGFKKS", "aa_hexdigest": "f7eb58a0e726f681ef33ce0e9201bbeb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000127407", "ncbi_nrp_id": "WP_010869308.1", "uniref100_id": "UniRef100_P47298", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000127407", "RefSeq:WP_010869308.1", "UniRef:UniRef100_P47298" ] }, "ips": { "uniref100_id": "UniRef100_P47298", "uniref90_id": "UniRef90_P47298" }, "psc": { "uniref90_id": "UniRef90_P47298", "gene": "cdd", "product": "cytidine deaminase", "ec_ids": [ "3.5.4.5" ], "uniref50_id": "UniRef50_P75051", "cog_id": "COG0295", "cog_category": "F", "kegg_orthology_id": "K01489", "go_ids": [ "GO:0004126", "GO:0005737", "GO:0005829", "GO:0008270", "GO:0008655", "GO:0009972", "GO:0042802" ] }, "pscc": { "uniref50_id": "UniRef50_P75051", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75051" ], "product": "Cytidine deaminase" }, "genes": [ "cdd" ], "id": "KMADJIBOEG_164", "locus": "GCA000292445_00124" }, { "type": "cds", "sequence": "contig_1", "start": 61399, "stop": 62346, "strand": "+", "frame": 1, "gene": "manB", "product": "Phosphomannomutase", "db_xrefs": [ "COG:COG1109", "COG:G", "EC:5.4.2.8", "GO:0000287", "GO:0004615", "GO:0005975", "GO:0006166", "GO:0008973", "SO:0001217", "UniRef:UniRef50_P47299", "UniRef:UniRef90_P47299" ], "nt": "ATGGATAAACTTAGATTAGAAGTTGAAAGATGGTTAAATCATCCTAATGTTAATTGGGAGTTAAAACAACAAATTAAGGAGTTGAATGAATCAGAAATTCAAGAACTTTTTAGTTTGGAAAAACCTTTATTTGGCACTGCAGGTGTAAGAAACAAAATGGCACCAGGTTATCATGGTATGAATGTTTTTTCTTATGCCTATTTGACCCAAGGTTATGTTAAGTACATTGAATCAATCAATGAACCAAAGCGTCAACTACGGTTTTTAGTAGCACGTGATACAAGAAAAAATGGTGGTTTATTTTTAGAAACGGTTTGTGATGTAATTACATCTATGGGTCATTTGGCTTATGTGTTTGATGATAACCAGCCAGTTTCAACACCTCTAGTGTCCCATGTCATTTTTAAATATGGTTTTAGTGGAGGTATTAATATCACAGCTAGCCATAACCCTAAAGATGATAATGGTTTTAAGGTTTATGATCATACTGGTGCACAGCTTTTAGACACACAAACAAACCAATTGTTAAGTGATTTACCTTGTGTTACATCTATGCTAGATTTGGAATTACAACCAAATCCAAAGTTTGTCCATACTCTTGACAATGAAAAGGTTTATAAAAACTATTTCAGAGAGTTGAAAAAGGTGTTGGTTATTAACAACAACAATTTCAAAGACATTAAGGTAGTTTTTAGTGGGCTTAATGGGACTTCAGTTTGCTTAATGCAACGCTTTTTAAAGTACCTTGGTTATAGCAATATTATCAGTGTTGAGGAACAAAATTGGTTTGATGAGAATTTTGAAAATGCTCCTAACTTAAATCCAGAGTATAAAGATACATGGATATTAGCACAAAAATATGCTAAGAAAAATAATGCTAAGTTAATTATTATGGCAGACCCTGATGCTGATAGATTTGCAATTGCAGAGTTAAATAATAATCAATGA", "aa": "MDKLRLEVERWLNHPNVNWELKQQIKELNESEIQELFSLEKPLFGTAGVRNKMAPGYHGMNVFSYAYLTQGYVKYIESINEPKRQLRFLVARDTRKNGGLFLETVCDVITSMGHLAYVFDDNQPVSTPLVSHVIFKYGFSGGINITASHNPKDDNGFKVYDHTGAQLLDTQTNQLLSDLPCVTSMLDLELQPNPKFVHTLDNEKVYKNYFRELKKVLVINNNNFKDIKVVFSGLNGTSVCLMQRFLKYLGYSNIISVEEQNWFDENFENAPNLNPEYKDTWILAQKYAKKNNAKLIIMADPDADRFAIAELNNNQ", "aa_hexdigest": "bc52937199f95df1025bd05d86d79105", "start_type": "ATG", "rbs_motif": null, "genes": [ "manB" ], "truncated": "3-prime", "pseudogene": { "start": 61399, "stop": 62649, "observations": { "mutation": [ 62344 ], "stop": [ 62344 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47299", "query_cov": 1.0, "subject_cov": 0.5727272727272728, "identity": 1.0, "score": 640.0, "evalue": 1.42e-228, "gene_start": 1, "gene_end": 315, "reference_start": 1, "reference_end": 315, "reference_sequence": "MDKLRLEVERWLNHPNVNWELKQQIKELNESEIQELFSLEKPLFGTAGVRNKMAPGYHGMNVFSYAYLTQGYVKYIESINEPKRQLRFLVARDTRKNGGLFLETVCDVITSMGHLAYVFDDNQPVSTPLVSHVIFKYGFSGGINITASHNPKDDNGFKVYDHTGAQLLDTQTNQLLSDLPCVTSMLDLELQPNPKFVHTLDNEKVYKNYFRELKKVLVINNNNFKDIKVVFSGLNGTSVCLMQRFLKYLGYSNIISVEEQNWFDENFENAPNLNPEYKDTWILAQKYAKKNNAKLIIMADPDADRFAIAELNNNQWHYFSGNETGAITAYYKLNHKVFKSPYIVSTFVSTYLVNKIAKRYGAFVHRTNVGFKYIGQAINELSQTNELVVGFEEAIGLITSDKLNREKDAYQAAALLLEIARHCKEQNITLLDFYKRILSEFGEYFNLTISHPFKATATDWKEEIKALFNQLINANLTEVAGFKVVKVHLDKQTNILEFGFENGWVKFRFSGTEPKLKFYFDLTNGTREALEKQAKKIYKFFVNLLKLNKA" }, "paralog": false, "identity": 0.9975961538461539, "score": 841.0, "evalue": 2.7e-310, "description": "Internal stop codon at 62344. Nonsense mutation around 62344.", "psc": { "uniref90_id": "UniRef90_P47299", "gene": "manB", "product": "Phosphomannomutase", "ec_ids": [ "5.4.2.8" ], "uniref50_id": "UniRef50_P47299", "cog_id": "COG1109", "cog_category": "G", "go_ids": [ "GO:0000287", "GO:0004615", "GO:0008973", "GO:0005975", "GO:0006166" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47299", "EC:5.4.2.8", "UniRef:UniRef50_P47299", "COG:COG1109", "COG:G", "GO:0000287", "GO:0004615", "GO:0008973", "GO:0005975", "GO:0006166" ] }, "pscc": { "uniref50_id": "UniRef50_P47299", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47299" ], "product": "Phosphomannomutase" } }, "id": "KMADJIBOEG_165", "locus": "GCA000292445_00125" }, { "type": "cds", "sequence": "contig_1", "start": 62452, "stop": 62778, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02880.22" ], "nt": "TTGGTAAATAAGATTGCTAAAAGATATGGCGCTTTTGTGCATAGAACCAATGTTGGTTTTAAGTACATTGGTCAAGCAATTAATGAGTTATCACAAACAAACGAATTAGTTGTTGGTTTTGAAGAGGCAATTGGTTTAATAACTAGTGATAAATTAAACCGCGAGAAAGATGCTTATCAAGCTGCTGCATTATTGCTTGAGATTGCTAGACATTGCAAAGAACAAAACATCACGCTTTTAGATTTTTATAAAAGAATTCTTTCTGAGTTTGGTGAATATTTCAATTTAACAATATCTCATCCCTTTAAAGCTACTGCTACTGATTGA", "aa": "MVNKIAKRYGAFVHRTNVGFKYIGQAINELSQTNELVVGFEEAIGLITSDKLNREKDAYQAAALLLEIARHCKEQNITLLDFYKRILSEFGEYFNLTISHPFKATATD", "aa_hexdigest": "136ea89dfccaa978498ac34c51588023", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02880.22", "name": "PGM_PMM_III", "length": 83, "aa_cov": 0.7685185185185185, "hmm_cov": 0.9036144578313253, "evalue": 5.872681306935725e-14, "score": 47.37049865722656, "start": 1, "stop": 83 } ], "seq_stats": { "molecular_weight": 12285.895599999985, "isoelectric_point": 6.831103324890138 }, "id": "KMADJIBOEG_166", "locus": "GCA000292445_00126" }, { "type": "cds", "sequence": "contig_1", "start": 62833, "stop": 63051, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00408.26" ], "nt": "GTGGCTGGTTTTAAAGTAGTTAAAGTCCATCTTGATAAACAAACAAATATCTTAGAGTTTGGTTTTGAAAATGGCTGGGTTAAATTCCGCTTTTCAGGTACTGAACCTAAATTGAAATTTTACTTTGACCTAACTAATGGCACTAGAGAGGCTCTAGAAAAGCAAGCTAAGAAAATTTATAAATTCTTTGTAAATTTACTCAAACTCAACAAAGCTTAA", "aa": "MAGFKVVKVHLDKQTNILEFGFENGWVKFRFSGTEPKLKFYFDLTNGTREALEKQAKKIYKFFVNLLKLNKA", "aa_hexdigest": "9704d7a0affff9369fc6f9fdfe87e693", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00408.26", "name": "PGM_PMM_IV", "length": 54, "aa_cov": 0.75, "hmm_cov": 0.9629629629629629, "evalue": 2.835260850759803e-08, "score": 28.900632858276367, "start": 7, "stop": 60 } ], "seq_stats": { "molecular_weight": 8461.833499999993, "isoelectric_point": 9.8733491897583 }, "id": "KMADJIBOEG_167", "locus": "GCA000292445_00127" }, { "type": "cds", "sequence": "contig_1", "start": 63028, "stop": 63549, "strand": "-", "frame": 1, "gene": "nusG", "product": "transcription termination/antitermination protein NusG", "db_xrefs": [ "COG:COG0250", "COG:K", "GO:0005829", "GO:0006353", "GO:0006355", "GO:0031564", "GO:0032784", "GO:0140673", "SO:0001217", "UniRef:UniRef50_P47300", "UniRef:UniRef90_P47300" ], "nt": "ATGCTTAAAGGGATATCAATCAATCCTAGCAAACGAATTATGCTAACAAATACTGCCATTATTGAAATGGACAGTGATAAATTTGATGAAAAATTTCAATATATCTTAAAACAAAAACAAGCCATTCAAAAACCAAAAGAGGATGAAGATTCAGAAATTGTTGATGCTGAAAAACTAAAAGAAGCTTTTAAAAAACTACAAAATAGTCAAGAACAAGATGAATGGAAAGAAAAAGCAACGATTATTCAAAGTGAGCAAACCAAACTTGATCCATCAGTATTAGTTCCTTTTTTGGGCAAATATGAAATTCTTGATACCGACAATAAAGTTGAACAACTCTTTGAATTTAGTGTTGGTAATTTAGTAGAGGTACATTTAACTGATACTATTCATGTTCAAGGACAGATAAAAGCACTTTATCAAGGTACAGTTAACAAAGCAGTTGTAGAGATAGAATTAACATCTAAAACCCAATTAATTAACTTACCTTTAGAAAATTTAAGCTTTGTTGAGTTTGAGTAA", "aa": "MLKGISINPSKRIMLTNTAIIEMDSDKFDEKFQYILKQKQAIQKPKEDEDSEIVDAEKLKEAFKKLQNSQEQDEWKEKATIIQSEQTKLDPSVLVPFLGKYEILDTDNKVEQLFEFSVGNLVEVHLTDTIHVQGQIKALYQGTVNKAVVEIELTSKTQLINLPLENLSFVEFE", "aa_hexdigest": "405919f1e35823e1132b4e0609bf5aca", "start_type": "ATG", "rbs_motif": null, "genes": [ "nusG" ], "truncated": "3-prime", "pseudogene": { "start": 63028, "stop": 63849, "observations": { "mutation": [ 63657 ], "stop": [ 63657 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47300", "query_cov": 1.0, "subject_cov": 0.5474683544303798, "identity": 1.0, "score": 326.0, "evalue": 1.67e-110, "gene_start": 1, "gene_end": 173, "reference_start": 144, "reference_end": 316, "reference_sequence": "MQASELTPKWYVAPVSIKDEAVVKNLKAKIQALGFNHEIVDVKVLKEREVHEEVYSLKSGKLPRSLKNTTFNKWFVLDDYRYLRVKISEKNLLGRYIYIKMIYSEDAWRIVRNFPGITGIVGSSGRGALPIPLDEKDANNLEQMLKGISINPSKRIMLTNTAIIEMDSDKFDEKFQYILKQKQAIQKPKEDEDSEIVDAEKLKEAFKKLQNSQEQDEWKEKATIIQSEQTKLDPSVLVPFLGKYEILDTDNKVEQLFEFSVGNLVEVHLTDTIHVQGQIKALYQGTVNKAVVEIELTSKTQLINLPLENLSFVEFE" }, "paralog": false, "identity": 0.9963369963369964, "score": 531.0, "evalue": 6.33e-194, "description": "Internal stop codon at 63657. Nonsense mutation around 63657.", "psc": { "uniref90_id": "UniRef90_P47300", "gene": "nusG", "product": "transcription termination/antitermination protein NusG", "uniref50_id": "UniRef50_P47300", "cog_id": "COG0250", "cog_category": "K", "go_ids": [ "GO:0005829", "GO:0006353", "GO:0006355", "GO:0031564", "GO:0032784", "GO:0140673" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47300", "UniRef:UniRef50_P47300", "COG:COG0250", "COG:K", "GO:0005829", "GO:0006353", "GO:0006355", "GO:0031564", "GO:0032784", "GO:0140673" ] }, "pscc": { "uniref50_id": "UniRef50_P47300", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47300" ], "product": "Transcription termination/antitermination protein NusG" } }, "id": "KMADJIBOEG_168", "locus": "GCA000292445_00128" }, { "type": "cds", "sequence": "contig_1", "start": 63655, "stop": 63978, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGCTAGTGAATTAACACCAAAGTGGTATGTAGCTCCTGTTAGTATTAAAGATGAAGCTGTTGTAAAAAATCTAAAAGCTAAAATTCAAGCTTTAGGATTTAATCATGAGATTGTTGATGTTAAAGTTCTAAAAGAAAGGGAAGTTCATGAAGAAGTTTATTCATTAAAATCAGGAAAACTTCCTCGTTCCTTAAAGAACACTACTTTTAACAAATGGTTTGTTCTTGATGATTACCGTTATCTTAGGGTAAAAATTAGTGAAAAAAATCTCCTTGGTAGATACATCTACATAAAGATGATTTATAGTGAAGATGCTTGA", "aa": "MQASELTPKWYVAPVSIKDEAVVKNLKAKIQALGFNHEIVDVKVLKEREVHEEVYSLKSGKLPRSLKNTTFNKWFVLDDYRYLRVKISEKNLLGRYIYIKMIYSEDA", "aa_hexdigest": "97401e671f986592ffc376eb3d641b5c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12579.512099999985, "isoelectric_point": 9.370753669738768 }, "id": "KMADJIBOEG_169", "locus": "GCA000292445_00129" }, { "type": "cds", "sequence": "contig_1", "start": 64138, "stop": 64353, "strand": "-", "frame": 1, "gene": "secE", "product": "preprotein translocase subunit SecE", "db_xrefs": [ "COG:COG0690", "COG:U", "GO:0005886", "GO:0008320", "GO:0009306", "GO:0015450", "GO:0016020", "GO:0043952", "SO:0001217", "UniRef:UniRef50_P47301", "UniRef:UniRef90_P47301" ], "nt": "ATGGAAAAAAAACTGCCTTTTAGCTTTAAAAAGAAGGAAAAGCTAACTGCTTATGATGATGCTTCAATTCATGAGTTACATAAACAGCTCAAACTTAGAACAGAAGCCAAGAAAAGTAAAGATAAGGAAAGAACTAAAGAAAAAGAAAAGCATGAAAGTTTAGCAAAGGAAAAGAAACCCAAGCTTCCTTTTAAAAAACGAATTGTTAATTTATGA", "aa": "MEKKLPFSFKKKEKLTAYDDASIHELHKQLKLRTEAKKSKDKERTKEKEKHESLAKEKKPKLPFKKRIVNL", "aa_hexdigest": "798ee827a94f4f5c9d633acf03b073b7", "start_type": "ATG", "rbs_motif": null, "genes": [ "secE" ], "truncated": "5-prime", "pseudogene": { "start": 63982, "stop": 64353, "observations": { "mutation": [ 64104, 64140 ], "stop": [ 64104, 64140 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47301", "query_cov": 1.0, "subject_cov": 0.5772357723577236, "identity": 1.0, "score": 112.0, "evalue": 9.56e-31, "gene_start": 1, "gene_end": 71, "reference_start": 1, "reference_end": 71, "reference_sequence": "MEKKLPFSFKKKEKLTAYDDASIHELHKQLKLRTEAKKSKDKERTKEKEKHESLAKEKKPKLPFKKRIVNLWFGVDKEINKIVWVKGRQLIIIFLLILLVSGLMVGIFFGINQLLITLGIFKN" }, "paralog": false, "identity": 0.983739837398374, "score": 210.0, "evalue": 7.15e-72, "description": "Internal stop codon at 64104, 64140. Nonsense mutation around 64104, 64140.", "psc": { "uniref90_id": "UniRef90_P47301", "gene": "secE", "product": "preprotein translocase subunit SecE", "uniref50_id": "UniRef50_P47301", "cog_id": "COG0690", "cog_category": "U", "go_ids": [ "GO:0005886", "GO:0008320", "GO:0009306", "GO:0015450", "GO:0016020", "GO:0043952" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47301", "UniRef:UniRef50_P47301", "COG:COG0690", "COG:U", "GO:0005886", "GO:0008320", "GO:0009306", "GO:0015450", "GO:0016020", "GO:0043952" ] }, "pscc": { "uniref50_id": "UniRef50_P47301", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47301" ], "product": "Uncharacterized protein MG055" } }, "id": "KMADJIBOEG_170", "locus": "GCA000292445_00130" }, { "type": "cds", "sequence": "contig_1", "start": 64661, "stop": 64903, "strand": "-", "frame": 3, "gene": null, "product": "MPN070 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75047", "UniRef:UniRef90_Q9ZB81" ], "nt": "ATGAAAGTATTTAAAAAACTCCATAACCTTAATGAGATTCTTTTGTTAATTAGTATCTTTTTTTTAGTTTGTATTATCTCTTTAGTAGGGATTGGTATCATCTTTGATTTAATTAGAAAAGCTAGTTTAAGTGCAATTAGAAGTGATCCTATTTTTTTTAATTTACGAGCAGTTTTAATTGTTTTAGGAGTTTTTGCTATCTGTTTTATGATCATCCAGTTAGTTATCAGCATAATGATTTGA", "aa": "MKVFKKLHNLNEILLLISIFFLVCIISLVGIGIIFDLIRKASLSAIRSDPIFFNLRAVLIVLGVFAICFMIIQLVISIMI", "aa_hexdigest": "72a53a11fe15a0e34b58945654560d0a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 64520, "stop": 64903, "observations": { "mutation": [ 64603, 64663 ], "stop": [ 64603, 64663 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9ZB81", "query_cov": 1.0, "subject_cov": 0.6299212598425197, "identity": 1.0, "score": 135.0, "evalue": 1.15e-39, "gene_start": 1, "gene_end": 80, "reference_start": 1, "reference_end": 80, "reference_sequence": "MKVFKKLHNLNEILLLISIFFLVCIISLVGIGIIFDLIRKASLSAIRSDPIFFNLRAVLIVLGVFAICFMIIQLVISIMIWKTINTACENIEPKFKKILHWSCFLPFGLLQLYCYQKIKLVKQADNL" }, "paralog": false, "identity": 0.984251968503937, "score": 241.0, "evalue": 1.15e-83, "description": "Internal stop codon at 64603, 64663. Nonsense mutation around 64603, 64663.", "psc": { "uniref90_id": "UniRef90_Q9ZB81", "product": "MPN070 family protein", "uniref50_id": "UniRef50_P75047", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9ZB81", "UniRef:UniRef50_P75047", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75047", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75047" ], "product": "Uncharacterized protein MG055.2 homolog" } }, "id": "KMADJIBOEG_171", "locus": "GCA000292445_00131" }, { "type": "cds", "sequence": "contig_1", "start": 64890, "stop": 64991, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATATTGGTGTTAAGTTAAAAGATGCATGCAATTATTTAGCTAAAAAAATGCATTTAAAATCAAGTATGCTCTATACACTTTTCCATGAAAGTATTTAA", "aa": "MDIGVKLKDACNYLAKKMHLKSSMLYTLFHESI", "aa_hexdigest": "52022b2b22d050a7493e856737549861", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3829.575, "isoelectric_point": 8.93288440704346 }, "id": "KMADJIBOEG_172", "locus": "GCA000292445_00132" }, { "type": "cds", "sequence": "contig_1", "start": 65124, "stop": 65723, "strand": "-", "frame": 2, "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "db_xrefs": [ "COG:COG0313", "COG:J", "EC:2.1.1.198", "GO:0000451", "GO:0005737", "GO:0008649", "GO:0008757", "GO:0070677", "KEGG:K07056", "SO:0001217", "UniRef:UniRef50_P47302", "UniRef:UniRef90_P47302" ], "nt": "ATGAAAACACTTAAAGTAGTTGCTACACCAATTGGTAATATTCAAGAGATAAGTGAAAGAGCTAAAAAAGCGTTACAAGATTGTGAAGTGTTATTCTGTGAAGATAGTAGAGTAACAAGAAAAATGCTGGATTTACTCAACATTGATTGCAAGCAAAAAAAGTTTGTTATTAACAACAGTTTTAAAGAAAAACAGAATTTAACTTTTGCTGAGGAATTTATAACTAACTTTAAGTGTTGTCTTGTTAGTGATGCAGGTTATCCTAGTTTATCTGATCCTGGTAATGAAATGATTAATTGGATCATATCAAAAAATAAGGAGATAAAAATCGAAGTTATTAATGGTCCTAGTGCATTAATGTGTGGATTGATCACTAGTGGTTTTAAAACAACACCACTTTTGTTTTTAGGTTTTTTAAGTCACAAACAAAATCAGCTCAAAAATTATTTAAGTACCTATCAAAATCAGAAAAGCACTATCGTTTTTTTTGAAGCAGTGCATAGGTTAGAAAATACTTTAGAAACTGTGAAAAATGTTTTTAAAAACAATGACGTTTTTATAGGAAGAGAATTAACTAAGTTACATGAATCACATTATTGA", "aa": "MKTLKVVATPIGNIQEISERAKKALQDCEVLFCEDSRVTRKMLDLLNIDCKQKKFVINNSFKEKQNLTFAEEFITNFKCCLVSDAGYPSLSDPGNEMINWIISKNKEIKIEVINGPSALMCGLITSGFKTTPLLFLGFLSHKQNQLKNYLSTYQNQKSTIVFFEAVHRLENTLETVKNVFKNNDVFIGRELTKLHESHY", "aa_hexdigest": "8d138a52482b83a29f059fc22b652a69", "start_type": "ATG", "rbs_motif": null, "genes": [ "rsmI" ], "truncated": "5-prime", "pseudogene": { "start": 64890, "stop": 65723, "observations": { "mutation": [ 65126 ], "stop": [ 65126 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47302", "query_cov": 1.0, "subject_cov": 0.7184115523465704, "identity": 0.995, "score": 399.0, "evalue": 1.06e-139, "gene_start": 1, "gene_end": 199, "reference_start": 1, "reference_end": 199, "reference_sequence": "MKTLKVVATPIGNIQEISERAKKALQDCEVLFCEDSRVTRKMLDLLNIDCKQKKFVINNSFKEKQNLTFAEEFITNFKCCLVSDAGYPSLSDPGNEMINWIISKNKEIRIEVINGPSALMCGLITSGFKTTPLLFLGFLSHKQNQLKNYLSTYQNQKSTIVFFEAVHRLENTLETVKNVFKNNDVFIGRELTKLHESHYWFNTSENTLPDITLKGEFVIVIDNQNINHQTLSSNQYLVYEIKKLMDIGVKLKDACNYLAKKMHLKSSMLYTLFHESI" }, "paralog": false, "identity": 0.9927797833935018, "score": 551.0, "evalue": 4.98e-202, "description": "Internal stop codon at 65126. Nonsense mutation around 65126.", "psc": { "uniref90_id": "UniRef90_P47302", "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "ec_ids": [ "2.1.1.198" ], "uniref50_id": "UniRef50_P47302", "cog_id": "COG0313", "cog_category": "J", "kegg_orthology_id": "K07056", "go_ids": [ "GO:0000451", "GO:0005737", "GO:0008649", "GO:0008757", "GO:0070677" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47302", "EC:2.1.1.198", "UniRef:UniRef50_P47302", "COG:COG0313", "COG:J", "KEGG:K07056", "GO:0000451", "GO:0005737", "GO:0008649", "GO:0008757", "GO:0070677" ] }, "pscc": { "uniref50_id": "UniRef50_P47302", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47302" ], "product": "Ribosomal RNA small subunit methyltransferase I" } }, "id": "KMADJIBOEG_173", "locus": "GCA000292445_00133" }, { "type": "cds", "sequence": "contig_1", "start": 65858, "stop": 66241, "strand": "-", "frame": 3, "gene": "rnmV", "product": "ribonuclease M5", "db_xrefs": [ "COG:COG1658", "COG:J", "EC:3.1.26.8", "GO:0005737", "GO:0006364", "GO:0019843", "GO:0043822", "GO:0046872", "KEGG:K05985", "SO:0001217", "UniRef:UniRef50_P75045", "UniRef:UniRef90_P47303" ], "nt": "ATGGATCAAAAAGCACGTATAAAAATTGATGGGGTAATTGTTTGTGAGGGTAAAACTGACCAAGCTAAATTACAGCAAATTTTTGATGTAGATGTAATTACAACAAATGGTTCAGCTTTAAAAAAAGAAACTATTAACCTAATTAAAAAAATCAGTGAAAAGCAAACTATTATCTTGTTATTAGATCCAGATCAACAAGGCAAAAAGATTAGAAGTAAATTAGAACAACATCTAACAAACTATTACAACTGTTATGTTGATATGAATGCAAGGTTAAAAAACGCTAAAAAAGCTGGTATTGCTGAAATGGAAACTAGTGCTTTAATTGCTGCTTTAAATAATCGTGTTGCAATCACAAAAAATGCTAATCAAAGCATATTATGA", "aa": "MDQKARIKIDGVIVCEGKTDQAKLQQIFDVDVITTNGSALKKETINLIKKISEKQTIILLLDPDQQGKKIRSKLEQHLTNYYNCYVDMNARLKNAKKAGIAEMETSALIAALNNRVAITKNANQSIL", "aa_hexdigest": "d7bab113f14fffb309fbbfe44371cc29", "start_type": "ATG", "rbs_motif": "ATATA", "genes": [ "rnmV" ], "truncated": "5-prime", "pseudogene": { "start": 65705, "stop": 66241, "observations": { "mutation": [ 65722, 65860 ], "stop": [ 65722, 65860 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47303", "query_cov": 1.0, "subject_cov": 0.7134831460674157, "identity": 0.992, "score": 244.0, "evalue": 4.53e-81, "gene_start": 1, "gene_end": 127, "reference_start": 1, "reference_end": 127, "reference_sequence": "MDQKARIKIDGVIVCEGKTDQAKLQQIFDVDVITTNGSALKKETINLIKKISEKQTVILLLDPDQQGKKIRSKLEQHLTNYYNCYVDMNARLKNAKKAGIAEMETSALIAALNNRVAITKNANQSILWDQYLSLKLNDKKKRLLLCNNLNLPYFNHKQLFKKLNLLNLTFQDVWKHLK" }, "paralog": false, "identity": 0.9831460674157303, "score": 342.0, "evalue": 3.93e-122, "description": "Internal stop codon at 65722, 65860. Nonsense mutation around 65722, 65860.", "psc": { "uniref90_id": "UniRef90_P47303", "gene": "rnmV", "product": "ribonuclease M5", "ec_ids": [ "3.1.26.8" ], "uniref50_id": "UniRef50_P75045", "cog_id": "COG1658", "cog_category": "J", "kegg_orthology_id": "K05985", "go_ids": [ "GO:0005737", "GO:0006364", "GO:0019843", "GO:0043822", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47303", "EC:3.1.26.8", "UniRef:UniRef50_P75045", "COG:COG1658", "COG:J", "KEGG:K05985", "GO:0005737", "GO:0006364", "GO:0019843", "GO:0043822", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P75045", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75045" ], "product": "Ribonuclease M5" } }, "id": "KMADJIBOEG_174", "locus": "GCA000292445_00134" }, { "type": "cds", "sequence": "contig_1", "start": 66220, "stop": 67311, "strand": "-", "frame": 1, "gene": "prs", "product": "Ribose-phosphate pyrophosphokinase", "db_xrefs": [ "COG:COG0462", "COG:F", "EC:2.7.6.1", "GO:0000287", "GO:0002189", "GO:0004749", "GO:0005524", "GO:0005737", "GO:0006015", "GO:0006164", "GO:0009156", "GO:0016301", "KEGG:K00948", "SO:0001217", "UniRef:UniRef50_P47304", "UniRef:UniRef90_P47304" ], "nt": "ATGGCAAAATTAAAGTGGAAATTTGATTGGCAAAACCTAAAAAGAAATTTGACAAACGTGAAACTATTAAAAAGAAAACAATCCGACGCGAGCTTGAAGCTGAGTATCGATAATAAAAAGCACGTTATTTTTAGTTTATCTAAAAGCAAAACACTGGTTGAAAATATCTGCAAAAAACTAAATATTTCAGAGGGCAAAATGGTTTGCGAACACTTTGCTGATGGTGAAACTTATATCCGTTTTGATGAATCAGTTCGTAACAAAGATATCTATATTTTTCAATCAACCTGTCCTAATGTTAACGATAGCTTAATGGAACTTTTAATTGCTATTGATGCATTGAAAAGAGGTAGTGCTAAAAGTATTACTGCCATTCTACCCTATTATGGATATGCAAGACAAGATAGAAAAACAAAAGGAAGAGAACCAATTACCAGTAAATTGATTGCTGATATGTTAACAAAAGCAGGTGCTAACAGGGTTGTTCTAACTGACATTCATAGTGATCAAACCCAAGGTTTTTTTGATATTCCCGTTGATTCTTTAAGAACTTATCACATCTTTCTTTTTAGAGTTATAGAACTACTTGGTAAAAAAGACTTGGTGGTTGTTTCCCCTGATTATGGTGGGGTTAAAAGAGCAAGGTTAATTGCAAATACACTAGAACTACCATTAGCCATTATTGATAAAAGAAGACCATCTCATAATGTTGCTGAATCAATTAATGTTTTAGGTGAAGTGAAAAATAAAAACTGTTTAATAGTTGATGACATGATAGATACTGGTGGTACAGTAATTGCAGCAGCCAAGCTATTACAAAAAGAACAAGCTAAAAAAGTGTGTGTAATGGCAACTCATGGTTTGTTTAACAATGATGCAGAACAAAAGTTTATGGAAACATTTGATCAAAAACTAATTGATTTCTTGTTTGTATCAAACTCTATTCCTCAATATAAGTTTAAAGCTGTAAAGCAGTTTGAAGTAGTTGATCTAGCATCTTTATATGAAGAGGTTGTTCTGTGTTACGCTAACAGCTTATCAGTTTCTGCAATATATGAACGTCACATTGAATGGATCAAAAAGCACGTATAA", "aa": "MAKLKWKFDWQNLKRNLTNVKLLKRKQSDASLKLSIDNKKHVIFSLSKSKTLVENICKKLNISEGKMVCEHFADGETYIRFDESVRNKDIYIFQSTCPNVNDSLMELLIAIDALKRGSAKSITAILPYYGYARQDRKTKGREPITSKLIADMLTKAGANRVVLTDIHSDQTQGFFDIPVDSLRTYHIFLFRVIELLGKKDLVVVSPDYGGVKRARLIANTLELPLAIIDKRRPSHNVAESINVLGEVKNKNCLIVDDMIDTGGTVIAAAKLLQKEQAKKVCVMATHGLFNNDAEQKFMETFDQKLIDFLFVSNSIPQYKFKAVKQFEVVDLASLYEEVVLCYANSLSVSAIYERHIEWIKKHV", "aa_hexdigest": "07d227f91ed372da1c206b8dcf63cbcb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47304", "query_cov": 0.9146005509641874, "subject_cov": 1.0, "identity": 0.9940000000000001, "score": 648.0, "evalue": 2.17e-234, "valid": true, "gene": "prs", "product": "Ribose-phosphate pyrophosphokinase", "ec_ids": [ "2.7.6.1" ], "uniref50_id": "UniRef50_P47304", "cog_id": "COG0462", "cog_category": "F", "kegg_orthology_id": "K00948", "go_ids": [ "GO:0005737", "GO:0002189", "GO:0005524", "GO:0016301", "GO:0000287", "GO:0004749", "GO:0006015", "GO:0006164", "GO:0009156" ] }, "pscc": { "uniref50_id": "UniRef50_P47304", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47304" ], "product": "Ribose-phosphate pyrophosphokinase" }, "genes": [ "prs" ], "id": "KMADJIBOEG_175", "locus": "GCA000292445_00135" }, { "type": "cds", "sequence": "contig_1", "start": 67286, "stop": 67636, "strand": "-", "frame": 3, "gene": "smpB", "product": "SsrA-binding protein", "db_xrefs": [ "COG:COG0691", "COG:O", "GO:0003723", "GO:0005829", "GO:0006450", "GO:0070929", "KEGG:K03664", "SO:0001217", "UniRef:UniRef50_P75043", "UniRef:UniRef90_P47305" ], "nt": "ATGTTAATTCTTGTTAACAATCCTAAGGCTAAATATGACTATCATTTAATGGAATCTTATTGTGCTGGAATAGTTTTAAAAGGAAGTGAAGTTAAAGCTTTAAGTTTAGGTCAAGGTAGTTTAAAAGAAGCTTATGTTTTTTTTAAAAACAATGAGCTTTTTTTAGAACAGTTCACTATTCCACCTTATAGTTTTGCAGGTCCATTAAATCACGCTTCAGATAGAATTAAGAAACTTTTATTAAATAAACATGAAATTAAACAAATTATCAATAAAAAACAACAACAATCTTTATCTGTAATCCCAAGTAAAGTATTCTTTAGAAATGGCAAAATTAAAGTGGAAATTTGA", "aa": "MLILVNNPKAKYDYHLMESYCAGIVLKGSEVKALSLGQGSLKEAYVFFKNNELFLEQFTIPPYSFAGPLNHASDRIKKLLLNKHEIKQIINKKQQQSLSVIPSKVFFRNGKIKVEI", "aa_hexdigest": "5205ad2c8d083fbc4fb46450169a4f80", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47305", "query_cov": 1.0, "subject_cov": 0.8, "identity": 0.991, "score": 226.0, "evalue": 9.81e-75, "valid": true, "gene": "smpB", "product": "SsrA-binding protein", "uniref50_id": "UniRef50_P75043", "cog_id": "COG0691", "cog_category": "O", "kegg_orthology_id": "K03664", "go_ids": [ "GO:0003723", "GO:0005829", "GO:0006450", "GO:0070929" ] }, "pscc": { "uniref50_id": "UniRef50_P75043", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75043" ], "product": "SsrA-binding protein" }, "genes": [ "smpB" ], "id": "KMADJIBOEG_176", "locus": "GCA000292445_00136" }, { "type": "cds", "sequence": "contig_1", "start": 67643, "stop": 67909, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00535.33" ], "nt": "ATGAAATTATCTGTAATTATACCTACTTACAATTGTGCATCATTTATTGAAAAAGCAATTAATTCAATTGTTAAAAATAGACCTAATGATTTGGAAATAGAAGTTTTAATTATTGATGATGGATCAATTGACAATACTAACAAAGTTATTAAGAAAATTCAAGACCAAATTAATAATTTAACTTTGCAGTATTTTTACAAAAGTAATGGTAACTGGGGTAGTGTTATTAATTATGTTAGAAACAATAAACTAGCAAAAGGGGAATGA", "aa": "MKLSVIIPTYNCASFIEKAINSIVKNRPNDLEIEVLIIDDGSIDNTNKVIKKIQDQINNLTLQYFYKSNGNWGSVINYVRNNKLAKGE", "aa_hexdigest": "26cec3462c71ae6ebe902523f5cd7737", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00535.33", "name": "Glycos_transf_2", "length": 84, "aa_cov": 0.9545454545454546, "hmm_cov": 0.9404761904761905, "evalue": 3.2959889196274187e-17, "score": 58.06611251831055, "start": 4, "stop": 87 } ], "seq_stats": { "molecular_weight": 10017.366199999995, "isoelectric_point": 8.732774543762208 }, "id": "KMADJIBOEG_177", "locus": "GCA000292445_00137" }, { "type": "cds", "sequence": "contig_1", "start": 67929, "stop": 68087, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATTTTTTCAAAAAAAACAATTTCTATTTTTCAAAAATATGCCCAAAAACAAAGATATGATGCGATTATTTTTGACTACTATAAATGCTGAAAAAAGTTTTTGTGAAAAATTCCTACCTATGCAAGGTTTAGAAAAGAAATTAAAGGTGAATTGA", "aa": "MTFFQKKQFLFFKNMPKNKDMMRLFLTTINAEKSFCEKFLPMQGLEKKLKVN", "aa_hexdigest": "941594f13135f7075475b6bd2801b7ba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6339.647499999999, "isoelectric_point": 10.01376132965088 }, "id": "KMADJIBOEG_178", "locus": "GCA000292445_00138" }, { "type": "cds", "sequence": "contig_1", "start": 68084, "stop": 68296, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAAACAAACACCTTTTTGTATTCCCTTAGCTAAGTTTTTTAAAAATGAGGTTTTCTATCAACTTCCTAAACTAAGAGAAAATGTTGGTTTTCAAGACGCTATTTATACGATGCATGCATTACAAATTGCAAATAATGTTTTCCATGTTTCTAAAGCTGGAGGATATTACTTTTTTAAAAGGGTAGGTAACTCTATGAGTATCCCTTGA", "aa": "MKKQTPFCIPLAKFFKNEVFYQLPKLRENVGFQDAIYTMHALQIANNVFHVSKAGGYYFFKRVGNSMSIP", "aa_hexdigest": "21a3cece4e3be010d522798d65e95ecf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8121.4417, "isoelectric_point": 9.779418754577634 }, "id": "KMADJIBOEG_179", "locus": "GCA000292445_00139" }, { "type": "cds", "sequence": "contig_1", "start": 68584, "stop": 69030, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCACTTCTTATTAACAGCTAACATAGTGGTATTGCTTATAATCATCATGTTTGCAGCAACACTTGGTATTGGTAGTGCAGCTGGGTTTGCTTTAATTAGTATCTTTTCATTTATCGGTGGTGCATTTGCTTGGAGTTTATCAAGTTCAAATTTAATCCTGCCATATGAATTTAAAGATTACAAGAAGAATGAACTTCCTATTCTTTTTGGATTTTGTTGGGGCTTTGGTTATATTGCTTACACCTTATTTGACATTACCCAATCAGTATTTCTCCAAGCGCCTGTTTTAATTCAAGGAAAAGGTACAAGTATTTTACCAGGGGTAATTGTAAGTATTGTTTTCTTCTTAGGACTTATTGCACTAGCAAACCTAATTGTTAAGTTCTTTCCTGCTTGTTGGATTAAAGATGGTGATCAATTGGTTCAGGAAATGACAAGAAAATGA", "aa": "MHFLLTANIVVLLIIIMFAATLGIGSAAGFALISIFSFIGGAFAWSLSSSNLILPYEFKDYKKNELPILFGFCWGFGYIAYTLFDITQSVFLQAPVLIQGKGTSILPGVIVSIVFFLGLIALANLIVKFFPACWIKDGDQLVQEMTRK", "aa_hexdigest": "f78a76e8d077db6d7b3fa4e0b2926108", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16253.248899999984, "isoelectric_point": 7.752804374694825 }, "id": "KMADJIBOEG_180", "locus": "GCA000292445_00140" }, { "type": "cds", "sequence": "contig_1", "start": 69262, "stop": 69972, "strand": "-", "frame": 1, "gene": null, "product": "Major facilitator superfamily transporter MG061", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47307", "UniRef:UniRef90_P47307" ], "nt": "TTGAGAGCAGTAGGTTCTATTCTTTGTGGAGTTGTTGTTTTGAAATTTGGTTATCGTTATGCGGTTTTAATCATGATGGGAATCATGTGTGTTTGTTTTCCCTTTCTTATTATAGGAGACCCTTTGAATGGTCATAATCAATTAACTTTATTAAGACCTTTATCAGATTCAGTAAAAACTCAATTAAGTTCACTAAGTAGCAATTTACAAGTGGGTCAATTACTTGGACCGGTGATGATCAATGGTAAAACGATGCTTGCAGATGGAACAAGTGTTGAACTTATTAAGGGTTTGGATGGTAATAGTATTGGTACAGCTGCATCAATTACTGGTTATGCATTGTTTATTATCTTTAGAAGCACAATTGCTATTGGTGGAACTACCTTAGTAGTTTATACTCAACCAGCAATTGCTAATCTTTCTTCAAACAGAAAAAAATCAATCCTATCTAATGCTAACCTTTGGGGTTTTAACATTGGGATTGCAGTAGTTTTCACACCATTTTTATTTGAACAAGTTCAACAAGTAGCATCTCAATACTGGGTTTACATTATGACTGTGATGATTTTGGTTGTGTTTGCTAACTTATGTTTATTTTTATGGTTTGAAAGCAAGATAGATCACATCTTTCCTCAAAAGCAAACTAAGGAAAATATGAGTTTAACTACTCAACCAAAATCCATAGATATCTTAAAGAATAAAACAACATGA", "aa": "MRAVGSILCGVVVLKFGYRYAVLIMMGIMCVCFPFLIIGDPLNGHNQLTLLRPLSDSVKTQLSSLSSNLQVGQLLGPVMINGKTMLADGTSVELIKGLDGNSIGTAASITGYALFIIFRSTIAIGGTTLVVYTQPAIANLSSNRKKSILSNANLWGFNIGIAVVFTPFLFEQVQQVASQYWVYIMTVMILVVFANLCLFLWFESKIDHIFPQKQTKENMSLTTQPKSIDILKNKTT", "aa_hexdigest": "5040f8586b191b71c17a1f3bf0093ea0", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 68959, "stop": 70218, "observations": { "mutation": [ 69033, 69105, 69201, 69264, 69987 ], "stop": [ 69033, 69105, 69201, 69264, 69987 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47307", "query_cov": 1.0, "subject_cov": 0.41622574955908287, "identity": 0.996, "score": 451.0, "evalue": 5.4e-155, "gene_start": 1, "gene_end": 236, "reference_start": 83, "reference_end": 318, "reference_sequence": "MENKSQKKVSDLKLITLWIIVIFGYLLFVVEWFVIDRISGKPTGILTQSTTTLPQYSGWLSSFFTENAGQIATSSTNWTITLLRAVGSILCGVVVLKFGYRYAVLIMMGIMCVCFPFLIIGDPLNGHNQLTLLRPLSDSVKTQLSSLSSNLQVGQLLGPVMINGKTMLADGTSVELIKGLDGNSIGTAASITGYALFIIFRSTIAIGGTTLVVYTQPAIANLSSNRKKSILSNANLWGFNIGIAVVFTPFLFEQVQQVASQYWVYIMTVMILVVFANLCLFLWFESKIDHIFPQKQTKENMSLTTQPKSIDILKNKTTWKLIGVYGIVLILIVNPLTPAWFSILQTVSPSGSNGLISTGVYTTGLATLAIFWVIGYALGFVVFSPFNKTIYDKKRWMHFLLTANIVVLLIIIMFAATLGIGSAAGFALISIFSFIGGAFAWSLSSSNLILPYEFKDYKKNELPILFGFCWGFGYIAYTLFDITQSVFLQAPVLIQGKGTSILPGVIVSIVFFLGLIALANLIVKFFPACWIKDGDQLVQEMTRKWKLNEWQFVIANKQKNRYSELLK" }, "paralog": false, "identity": 0.9856801909307876, "score": 796.0, "evalue": 1.42e-293, "description": "Internal stop codon at 69033, 69105, 69201, 69264, 69987. Nonsense mutation around 69033, 69105, 69201, 69264, 69987.", "psc": { "uniref90_id": "UniRef90_P47307", "product": "Major facilitator superfamily transporter MG061", "uniref50_id": "UniRef50_P47307", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47307", "UniRef:UniRef50_P47307", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47307", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47307" ], "product": "Major facilitator superfamily transporter MG061" } }, "id": "KMADJIBOEG_181", "locus": "GCA000292445_00141" }, { "type": "cds", "sequence": "contig_1", "start": 69985, "stop": 70218, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAAATAAAAGTCAAAAAAAAGTAAGTGATCTGAAACTTATTACCCTGTGGATCATAGTAATTTTTGGGTATCTATTGTTTGTAGTTGAATGGTTTGTAATTGATCGAATTAGTGGTAAACCAACTGGTATACTTACTCAATCAACCACCACTTTACCACAGTACAGTGGTTGGTTAAGTTCTTTCTTTACTGAAAATGCTGGGCAAATAGCCACATCTTCCACCAACTGA", "aa": "MENKSQKKVSDLKLITLWIIVIFGYLLFVVEWFVIDRISGKPTGILTQSTTTLPQYSGWLSSFFTENAGQIATSSTN", "aa_hexdigest": "9f8e71f0f49ac5cd20d65e72a80bf55c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8660.895099999994, "isoelectric_point": 8.06423740386963 }, "id": "KMADJIBOEG_182", "locus": "GCA000292445_00142" }, { "type": "tRNA", "sequence": "contig_1", "start": 70386, "stop": 70474, "strand": "-", "gene": "trnS", "product": "tRNA-Ser(gct)", "amino_acid": "Ser", "anti_codon": "gct", "score": 76.9, "nt": "GGGAGCGTACTCAAGTGGTTAAGAGGACACCCTGCTAAGGTGTTAGATCGTTCTACGGTGCGTGGGTTCGAATCCCACCGCTTCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 70439, 70441 ], "id": "KMADJIBOEG_34", "locus": "GCA000292445_00143" }, { "type": "cds", "sequence": "contig_1", "start": 70523, "stop": 70789, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00359.27" ], "nt": "ATGTTTAAAAATTTATTAAGACCATCCCTATTTTTTAACTGGAGCCAAAAAACATTTAAAAATAAATTTTCGTTTTTAAAACAAGCAGCAAATGCATTACAAAAACAGGCTGTTATCAATGATAACAATGTTGCATTTGAAGCTTTAAAAAAGCGTGAAGAAGAGATTACAACTGGAATTATTACTAGTTTAGCTTTACCTCACTTACAAAGTCAAAGTGTTATAGAACCTTTTGTTGCTGTATTTAAGGTTAAAAACTTAGATTGA", "aa": "MFKNLLRPSLFFNWSQKTFKNKFSFLKQAANALQKQAVINDNNVAFEALKKREEEITTGIITSLALPHLQSQSVIEPFVAVFKVKNLD", "aa_hexdigest": "69befee4b3c9dac3ce986b5e98e60c3f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00359.27", "name": "PTS_EIIA_2", "length": 71, "aa_cov": 0.8068181818181818, "hmm_cov": 1.0, "evalue": 1.3282900739743505e-14, "score": 49.511775970458984, "start": 16, "stop": 86 } ], "seq_stats": { "molecular_weight": 10084.609399999996, "isoelectric_point": 9.821838951110838 }, "id": "KMADJIBOEG_183", "locus": "GCA000292445_00144" }, { "type": "cds", "sequence": "contig_1", "start": 70817, "stop": 71359, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02302.22" ], "nt": "TTGATATTTTTAATTGGTGTTCCTGTTGACAAAACCAATTTGCATCTTGATTTCATTAGTAACTTTTCCAAGTTAATGTTGAATGAAACATTTGCAAGTAAGGTTTTAAATGTCACTAGCTATAACGGCTTGATTAAACTAATTGATCTTTTTAACCAACAAAAAGTGCAAGACCAACCAGCTGTTGAAACAAAAAAAGAGTATGACTTTGTTGCTGTAACAGCATGCCCAACTGGCATTGCCCATACTTTCATGGCTAAAGAAGCGTTGGAAGCATTTGCAAAAAAGCATAACTTATATGTAAAAGTTGAAACTCAAGGTACAGATGGGATACAAAATCAACTTACAAGTGACGATATTAATAACGCTAAAGGTGTTATTCTTGCTTGTGATCGTTTAATTGATTTCTCTAGGTTTTATGCAAATAAGAATGTGATTGAAGTGTCAACTCTAAAAGCCATTAAAAAACCTGATGAGGTGTATGAATTAATTAAAAACCAAAAGGGTAAACAGCTTGCAAATAGTGCTAAACCAACTAATTAA", "aa": "MIFLIGVPVDKTNLHLDFISNFSKLMLNETFASKVLNVTSYNGLIKLIDLFNQQKVQDQPAVETKKEYDFVAVTACPTGIAHTFMAKEALEAFAKKHNLYVKVETQGTDGIQNQLTSDDINNAKGVILACDRLIDFSRFYANKNVIEVSTLKAIKKPDEVYELIKNQKGKQLANSAKPTN", "aa_hexdigest": "511d05608242bae3c20a9e974bd79703", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02302.22", "name": "PTS_IIB", "length": 96, "aa_cov": 0.5111111111111111, "hmm_cov": 0.9270833333333334, "evalue": 6.144944366729772e-21, "score": 70.07487487792969, "start": 71, "stop": 162 } ], "seq_stats": { "molecular_weight": 20128.94840000001, "isoelectric_point": 8.41617031097412 }, "id": "KMADJIBOEG_184", "locus": "GCA000292445_00145" }, { "type": "cds", "sequence": "contig_1", "start": 71447, "stop": 72565, "strand": "+", "frame": 2, "gene": "fruA", "product": "PTS system fructose-specific EIIABC component", "db_xrefs": [ "COG:COG1762", "COG:GT", "GO:0005351", "GO:0005886", "GO:0009401", "GO:0016301", "GO:0022877", "GO:0090563", "SO:0001217", "UniRef:UniRef50_P47308", "UniRef:UniRef90_P47308" ], "nt": "ATGCTACCATTCGTTGTTTTTGGTGGGATATTAATTGCACTCTCTTTTCTAATTGATATAAATAACGCTAATAATGCTGGTGGTAACTTTGGCACAATTAATCCTGTTGCTAACTGGTTAAATAAGCTAGGAGGAATTTCCTTTAGTTTAATTGTTCCTATCTTATCTGCATACATTGCATATGCATTGGTATCAAGACAGGGATTATTACCTGGTTTTGTTGTTGGTTTAATCTCTTCAGGACAATTTTTATTAAATATTGTTCTGACCAATGGAACAATTGAATGGTTAGCACCATCACAAGTATCAAGTGGCTTTTTTGGTGCTATCTTTGGAGGACTTTTGAGTGCTTGCTTAATAATTGTTCAACAAAACTACATCTATAAAAAATTACCTCAATCTTTGCAGGGAATTAAAAACATCTTGTTTATTCCCTTGTTTGGTACTTTATTCACTGCTGGTTTATTTTGGGTTATTAACATTCCTTTAATTTATCTAAACTATGGATTAAGTCTGTTTTTAAATATTATGAACAGCCCTATCCTAGCACCTTTACTTGGTTTTGTAATTGGGTTGATGATGTGCTTTGATTTAGGGGGGCCAATTAATAAAGCAGCTTATGTTTTTGGTGTTGTTTCTTTACAAAATCAAAATGCAGGAACAATTGCGATGGCTGCAGCTATGCTATCAGGGATGGTACCTCCTTTATCAATTGCTTTGGCAGCTTCCATTCGAAAGAGCTGCTTTGATAAACAGGAATTACCTGCAGCTTATGCTTGTTATCTGATGGGATTGAGTTTTATTAGTGAAGGTGCTATCCCATTTGTTGTTAAAAAACCTAAGGTGATGTTAACTGCTAACTTAATTGCTGGAGCAATTTGTGGAGCATTAACAGGAGCATTTGCCTTATCAATTCGTGCTCCTCATGGCGGTGTTTTTGTGTTTGCACTTTTAAAAACTACTTTACAAGGGATTGAAGGAGCTACATTACAAACTGGAGTTGGCATTGGTTTGGCATTGGTTTGTTTAATAATTAGTATGATAGTTGGTAGTAGTATTATCATTGGCTATGACTTGATTGCAAAACATAACCAAAGAAAGCAAAATCTGAATAGTTAA", "aa": "MLPFVVFGGILIALSFLIDINNANNAGGNFGTINPVANWLNKLGGISFSLIVPILSAYIAYALVSRQGLLPGFVVGLISSGQFLLNIVLTNGTIEWLAPSQVSSGFFGAIFGGLLSACLIIVQQNYIYKKLPQSLQGIKNILFIPLFGTLFTAGLFWVINIPLIYLNYGLSLFLNIMNSPILAPLLGFVIGLMMCFDLGGPINKAAYVFGVVSLQNQNAGTIAMAAAMLSGMVPPLSIALAASIRKSCFDKQELPAAYACYLMGLSFISEGAIPFVVKKPKVMLTANLIAGAICGALTGAFALSIRAPHGGVFVFALLKTTLQGIEGATLQTGVGIGLALVCLIISMIVGSSIIIGYDLIAKHNQRKQNLNS", "aa_hexdigest": "939624a756d92c4d644fd6530cd8ae43", "start_type": "ATG", "rbs_motif": "ATTTTA", "genes": [ "fruA" ], "truncated": "5-prime", "pseudogene": { "start": 71147, "stop": 72565, "observations": { "mutation": [ 71357 ], "stop": [ 71357 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47308", "query_cov": 1.0, "subject_cov": 0.5470588235294118, "identity": 0.997, "score": 704.0, "evalue": 7.95e-251, "gene_start": 1, "gene_end": 372, "reference_start": 309, "reference_end": 680, "reference_sequence": "MFKNLLRPSLFFNWSQKTFKNKFSFLKQAANALQKQAVINDNNVAFEALKKREEEITTGIITSLALPHLQSQSVIEPFVAVFKVKNLDWQSLDQKPVKLIFLIGVLVDKTNLHLDFISNFSKLMLNETFASKVLNVTSYNGLIKLIDLFNQQKVQDQPAVETKKEYDFVAVTACPTGIAHTFMAKEALEAFAKKHNLYVKVETQGTDGIQNQLTSDDINNAKGVILACDRLIDFSRFYANKNVIEVSTTKAIKKPDEVYELIKNQKGKQLANSAKPTNQTQLAESEGEFNFNNFHKRIYRAILSGVSYMLPFVVFGGILIALSFLIDINNANNAGGNFGTINPVANWLNKLGGISFSLIVPILSAYIAYALVSRQGLLPGFVVGLISSGQFLLNIVLTNGTIEWLAPSQVSSGFFGAIFGGLLSACLIIVQQNYIYKKLPQSLQGIKNILFIPLFGTLFTAGLFWVINIPLIYLNYGLSLFLNIMNSPILAPLLGFVIGLMMCFDLGGPINKAAYVFGVVSLQNQNAGTISMAAAMLSGMVPPLSIALAASIRKSCFDKQELPAAYACYLMGLSFISEGAIPFVVKKPKVMLTANLIAGAICGALTGAFALSIRAPHGGVFVFALLKTTLQGIEGATLQTGVGIGLALVCLIISMIVGSSIIIGYDLIAKHNQRKQNLNS" }, "paralog": false, "identity": 0.9936440677966102, "score": 913.0, "evalue": 0.0, "description": "Internal stop codon at 71357. Nonsense mutation around 71357.", "psc": { "uniref90_id": "UniRef90_P47308", "gene": "fruA", "product": "PTS system fructose-specific EIIABC component", "uniref50_id": "UniRef50_P47308", "cog_id": "COG1762", "cog_category": "GT", "go_ids": [ "GO:0005886", "GO:0005351", "GO:0016301", "GO:0022877", "GO:0090563", "GO:0009401" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47308", "UniRef:UniRef50_P47308", "COG:COG1762", "COG:GT", "GO:0005886", "GO:0005351", "GO:0016301", "GO:0022877", "GO:0090563", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P47308", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47308" ], "product": "PTS system fructose-specific EIIABC component" } }, "id": "KMADJIBOEG_185", "locus": "GCA000292445_00146" }, { "type": "cds", "sequence": "contig_1", "start": 72660, "stop": 73427, "strand": "+", "frame": 3, "gene": "fruK", "product": "Putative 1-phosphofructokinase", "db_xrefs": [ "COG:COG1105", "COG:G", "EC:2.7.1.56", "GO:0005524", "GO:0005829", "GO:0008443", "GO:0008662", "SO:0001217", "UniRef:UniRef50_Q49396", "UniRef:UniRef90_Q49396" ], "nt": "ATGAAATCACTTGGTTTTGATCCAACTGTCATTACTTTTTTGGGACAACCCACTAAAAACTTATTTTTAGAGTTGGTAAAACCTTATGATCTAAATATAGTTAGCTTCATTTCTGAAACTAAAACAAGAATTAACCTTAAGTTATTAAAAGATGAAAAAACTACTGAAATTAATGATTTAAGTCCTTTAATAACAGATGCTAATCTAACTGAATTGTTAACTTTTTTAAAAGCTAATGTTAAGAATAATGATTTGGTTATCATCAACGGAAGATTTAAATTTGAAGCTTTAGAAAAAGTTCTAAACTTGGTCTTTACATTAACAGAAAATGTGGTTATAGATGTTGATGAAAGCAAAATGTTAACGCTCTTAAATCAGTCTAAACCACTAGTTATGAAACCTAACATTGATGAGTTTCAAACTATGATTAATACTTTTTTTCAAGATCAACAAAGCTTAATAGCAGCAATTAAAAAATTTCATTACTGTAAGCTCTTGTTATTATCTGATGGTGACAAAGGAGCTTATCTTTTTGATCAGAATAAGTTATTGTTTGTAAGTTCTATCACTCCTAAACAAGTAGTTAGCACCACAGGAGCAGGTGATACTTTGTTGGCAGTTTTTTTAGCAAATTTGATTCTAAAGGTAGATTTAAAAACTGCTTTGATTAAAGCAACTAACTATGCAAGTGCAACAATTAGTAAGTTAGGTGTTGTTGATAGTAAAGACAAAATTAGTGTTATAATCCCAAAAAGTTACTATTTATAA", "aa": "MKSLGFDPTVITFLGQPTKNLFLELVKPYDLNIVSFISETKTRINLKLLKDEKTTEINDLSPLITDANLTELLTFLKANVKNNDLVIINGRFKFEALEKVLNLVFTLTENVVIDVDESKMLTLLNQSKPLVMKPNIDEFQTMINTFFQDQQSLIAAIKKFHYCKLLLLSDGDKGAYLFDQNKLLFVSSITPKQVVSTTGAGDTLLAVFLANLILKVDLKTALIKATNYASATISKLGVVDSKDKISVIIPKSYYL", "aa_hexdigest": "8efea2d3462fab8f882d7bf68696617d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q49396", "query_cov": 1.0, "subject_cov": 0.8415841584158416, "identity": 0.992, "score": 481.0, "evalue": 1.88e-170, "valid": true, "gene": "fruK", "product": "Putative 1-phosphofructokinase", "ec_ids": [ "2.7.1.56" ], "uniref50_id": "UniRef50_Q49396", "cog_id": "COG1105", "cog_category": "G", "go_ids": [ "GO:0005829", "GO:0008662", "GO:0005524", "GO:0008443" ] }, "pscc": { "uniref50_id": "UniRef50_Q49396", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49396" ], "product": "Putative 1-phosphofructokinase" }, "genes": [ "fruK" ], "id": "KMADJIBOEG_186", "locus": "GCA000292445_00147" }, { "type": "cds", "sequence": "contig_1", "start": 73438, "stop": 73641, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_014894126.1", "SO:0001217", "UniParc:UPI00027B32C6", "UniRef:UniRef100_UPI00027B32C6", "UniRef:UniRef50_UPI00027B32C6", "UniRef:UniRef90_UPI00027B32C6" ], "nt": "ATGGTAAAAAGAAAGCGAAAGCCTAAGCTTAATTCGCGTAATATTTTAACTATCCAGATTGTTTTAGCAATCTTTAGTATGATCTTTTTTCTTACTTTGCTATCTTTAATCTTGTTTTTGAGTTTGCAAAGTAATTTAGCTACAGCTTTAGTTGAAAACAGAAATAAAGCTGTGGAACTTGTAGATAACATTGTCTTTTTTTAA", "aa": "MVKRKRKPKLNSRNILTIQIVLAIFSMIFFLTLLSLILFLSLQSNLATALVENRNKAVELVDNIVFF", "aa_hexdigest": "3a71b4558c207c861345ffead1398d13", "start_type": "ATG", "rbs_motif": "ATTTA", "ups": { "uniparc_id": "UPI00027B32C6", "ncbi_nrp_id": "WP_014894126.1", "uniref100_id": "UniRef100_UPI00027B32C6", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B32C6", "RefSeq:WP_014894126.1", "UniRef:UniRef100_UPI00027B32C6" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B32C6", "uniref90_id": "UniRef90_UPI00027B32C6" }, "psc": { "uniref90_id": "UniRef90_UPI00027B32C6", "uniref50_id": "UniRef50_UPI00027B32C6" }, "pscc": { "uniref50_id": "UniRef50_UPI00027B32C6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B32C6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7721.306799999995, "isoelectric_point": 11.0533784866333 }, "id": "KMADJIBOEG_187", "locus": "GCA000292445_00148" }, { "type": "cds", "sequence": "contig_1", "start": 73683, "stop": 74573, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAAACCTAATTAAAAATGTTATTCGTTCTTTAAAGAGTGCTAAGATTGCTTTAATAGCGTTAACTTTTTTAATTTTTGTTGCTGTTGGTGGTTTTGTGTTGTTAAATAACACAGTTAATAATTTTAACGCTGCTTTTAACTATGTTACCCACACTGGTAAATTAAGTAATGCCATCATTAATGAACGTTATGACTTTGGTAAATTAGAGTTTCAAGAACAGACCAATAATTCTCAGAATAGTAGCGACAGTTTTACTTTAACTTTAACTAACGATTCAAGAACAAGTTTTATTAATAATGCTTTGAGAACTAACCCTTCTTTGTATGAAGGATTAGTAACCCAAACTTTTAGTTATCAAAACAAAACTGAAATGACTGAAAAAACCAATATAGTTAATCAGTCTAAAATTATTGCTGCTAACAATCTTAACAATGCATTAAGTAAAGATAAACAGCTCTTAGTTTCAGGCCAACTTGAAAAACTAAATGCTGTTTTTCGAGAATATAAAGCTATTAATATTACTGACAAAAGTGTTTTTAAAAAATTGATAGTATCAGAACCTAATGATTTGGTAAATAGCCTAGTTATTTTTGATGGTCAAAATTTATCTCTCTCCAAACAAAGTTATTTCAATAATTTTTTAAATCAATTTAACGAAATTAAATCAAAGGGTAAAGATAATTTAAGTACTACTTTAAAAACTGGGCAGTATCAGGCATTTTTACAAACTCTTTTTGATTATGTTCAAGCGAGTGAAACAACATTAAAAGATCAGCTTCAAAAGTTAATTTCAAATCCAGATTCAAGTGAAACAAATCAGGTTAAAAATCTCTTTGATACTCCAAGTGCGCTTACTAATATTGGGGGTCAATTAACCTTACAATGA", "aa": "MLNLIKNVIRSLKSAKIALIALTFLIFVAVGGFVLLNNTVNNFNAAFNYVTHTGKLSNAIINERYDFGKLEFQEQTNNSQNSSDSFTLTLTNDSRTSFINNALRTNPSLYEGLVTQTFSYQNKTEMTEKTNIVNQSKIIAANNLNNALSKDKQLLVSGQLEKLNAVFREYKAINITDKSVFKKLIVSEPNDLVNSLVIFDGQNLSLSKQSYFNNFLNQFNEIKSKGKDNLSTTLKTGQYQAFLQTLFDYVQASETTLKDQLQKLISNPDSSETNQVKNLFDTPSALTNIGGQLTLQ", "aa_hexdigest": "3bb96c8ee37f8e6c7da393eab19aff2c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 33133.00700000004, "isoelectric_point": 9.03081188201904 }, "id": "KMADJIBOEG_188", "locus": "GCA000292445_00149" }, { "type": "cds", "sequence": "contig_1", "start": 75078, "stop": 75386, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02687.27" ], "nt": "TTGAATTTATTAGCAGCAAGAACAGTTTTTATCCCTAATCTAATTAACACAATTAATTTAGTGGCTTTGTTTTTAACTATTGCTATCCTAACTGTTGCTATAATTGTCAGCATTTTAATCCTGATTAGTTATTTAAAGAAAAACACTGAGCAAATTGGCATTTTAAAAGCAAATGGGTTAAGTGGTAAAAAGATTAACCTTAGCTTGTTAATCTTTGGGTTAATTCCTGCTATAGTAGGTGCTATTTCTGGATATAGCTTTGGAATTGGATTTCAAGACGTAGCTATTCATCTATTTAGTAACTATTGA", "aa": "MNLLAARTVFIPNLINTINLVALFLTIAILTVAIIVSILILISYLKKNTEQIGILKANGLSGKKINLSLLIFGLIPAIVGAISGYSFGIGFQDVAIHLFSNY", "aa_hexdigest": "7259c36bcdd016408920d3f67883d822", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02687.27", "name": "FtsX", "length": 74, "aa_cov": 0.7156862745098039, "hmm_cov": 1.0, "evalue": 7.961999704103435e-11, "score": 37.230735778808594, "start": 24, "stop": 96 } ], "seq_stats": { "molecular_weight": 10943.102899999989, "isoelectric_point": 9.777226829528807 }, "id": "KMADJIBOEG_189", "locus": "GCA000292445_00150" }, { "type": "cds", "sequence": "contig_1", "start": 75429, "stop": 75635, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTTTTTCACTGTTTGTTATCTTAATTATGAGTAGTATATCGCTTTTAGTGGGATCAATTATCTTAAAGAAGGATGTTGTAAAGATTTTAAAGCACGACAGTGAATTTAAAGTTTCAAGATTAGGGCTTAGTTCTAAGAAATTGTTTGCTAGGTTTGGTATTATGACCAGGTTTAGAGTAGCATTAGCATTTAACGCTCCTTGA", "aa": "MFFSLFVILIMSSISLLVGSIILKKDVVKILKHDSEFKVSRLGLSSKKLFARFGIMTRFRVALAFNAP", "aa_hexdigest": "8beeb4b01f9c33f95dd04916f08798f9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7677.318799999996, "isoelectric_point": 11.13389949798584 }, "id": "KMADJIBOEG_190", "locus": "GCA000292445_00151" }, { "type": "cds", "sequence": "contig_1", "start": 75657, "stop": 75809, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTCATTTACAATGATGATTTTAAACCTTAGTTTTGCAACTAAAGATAGCTTTGAAAATGCTCAATCAAAAACTAATTTAACTAATCAGAACCACCAATATGAATTTGAACTCGCTTCAGCAACAACACAAAGTGGTTTATTGAAGTGA", "aa": "MSSFTMMILNLSFATKDSFENAQSKTNLTNQNHQYEFELASATTQSGLLK", "aa_hexdigest": "7a78ee8852ef66890264e9313cd3a180", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5620.217699999999, "isoelectric_point": 5.510108375549315 }, "id": "KMADJIBOEG_191", "locus": "GCA000292445_00152" }, { "type": "cds", "sequence": "contig_1", "start": 76071, "stop": 76565, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAACTAACCAAGCATCTGCTTCCAACACTGCTTTTCAAAATTTTTTAAAAGCAATCATTACTATAAATCCAAGCCAAGGATCGCAATTCATTAAACAAACCCAAGATCCATTAACAAAAAGATTTATCTATGCAATTGACAGTGATAAGGCATTAAAAAATAATAATGAACAAAACGGTTCCCAAAACCACTTAACTTTAAATGATGATTTTGCTAAATTTCTCTACAGTCAATTTGAATTAATTAAAAAGAGTGGGAATGCAAGTAATGAAGATTTAAATGCAATTGATTTTGAAAACCCCCAAACAATCAGAGATTTTTACAACAAGTACAATGCTTTACCACCATTAGATTACAAACTTAGCTTTAATGTAATAGGTTTACCCAAAGAGACAATTGCTGGACAAATTGACACCCCTAAGTATGGATTTTTAACCCTTCATGGTGAATATCAAAATACTCCTATCAAGATTAAAGGTATTAAAGATTGA", "aa": "MPTNQASASNTAFQNFLKAIITINPSQGSQFIKQTQDPLTKRFIYAIDSDKALKNNNEQNGSQNHLTLNDDFAKFLYSQFELIKKSGNASNEDLNAIDFENPQTIRDFYNKYNALPPLDYKLSFNVIGLPKETIAGQIDTPKYGFLTLHGEYQNTPIKIKGIKD", "aa_hexdigest": "d6c71fb82a4e129340b4ab333a220b62", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18527.53399999999, "isoelectric_point": 6.73123722076416 }, "id": "KMADJIBOEG_192", "locus": "GCA000292445_00153" }, { "type": "cds", "sequence": "contig_1", "start": 76575, "stop": 77630, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0011815B2F" ], "nt": "GTGGATAATTTAGGTCCAGTTTTGAGTGATCAAAACAACCACATTATTAATCAAGAATTGTTTAAAAATTATTCTTTTGATCCTTTGATAGTTAACAATTCTGCTGCAAAAAAATACCAACTTGCAATAGGTAGTGAGATTAATATTGCAGTTAACAACAGCTTCAAACGGATTGACAATAAGATCATTAATCAAGATCCTTTAGTGAATGCTACCTTTAGAGTTGTAGGGATTAACAATTCCGCTCATGATCCTGAATTTTTCACTAGTTATAGTACTGCTTTTAAAGTATTGGAATATCCCAATGAATGGTTCGTAAAAAAACTTCCATTTAATAGCTTCTATGCTAATTCGCTTTTAAGTTTTGTTCAATCTACTTCGCTATTTTCTGAATCTGGTATTTTTCCTGCTACTAGTAGTTTTTCAACTAATAACACTGTACTTGTTGAGTTAATTAAAAAAACCATTAATTACAAGAATGGTCAAATGAATCAAACTTCAAGTAATGACTCTTCTAAGAAAGAAAATTACCAAAAATTGCAAAAAGCATTAGGAATATCAACTGATTTGGAGATTAGTAAAGTTAATGAATATGTTGCTATCTTAGCAAGGGTTTATAATGGTTTACCTTACAACTCTACTATTAGCTTTATTAGCAATGTTGCTGCTAACAACGCTTTATTTGGAAATATTGCTAACACCACCAAGCAGATTCAAGCTGTTGTAATTGCAGTGATTATTCCTATAATCATGTTGATTATTCTTTTGGTTTCAACTACCTTAATTCAAGAGTTGAAAAAAATTGCTATTAGATTAAAAGCATTGGGATATTCCAATTTAAAAATTCTCGCTTCATTTTTATCAATATACATCCCTTTATTTGCCTTTGGTTTGTTGATTTCTATCCCCTTTTCTATCTATCTAATTGCACTACATAATGAGGTAATTTTTGCAAGCTCATCGATCTTTTTAGATGCTTTTTTAAGTTTTGAAAGTGCAATTGGTTCAATGTTAGTTTTACTAGCGGTTTTATCAATTACCTTTGTGTTGAATTGA", "aa": "MDNLGPVLSDQNNHIINQELFKNYSFDPLIVNNSAAKKYQLAIGSEINIAVNNSFKRIDNKIINQDPLVNATFRVVGINNSAHDPEFFTSYSTAFKVLEYPNEWFVKKLPFNSFYANSLLSFVQSTSLFSESGIFPATSSFSTNNTVLVELIKKTINYKNGQMNQTSSNDSSKKENYQKLQKALGISTDLEISKVNEYVAILARVYNGLPYNSTISFISNVAANNALFGNIANTTKQIQAVVIAVIIPIIMLIILLVSTTLIQELKKIAIRLKALGYSNLKILASFLSIYIPLFAFGLLISIPFSIYLIALHNEVIFASSSIFLDAFLSFESAIGSMLVLLAVLSITFVLN", "aa_hexdigest": "f9180b4f61982dc951b184d8cb4df88e", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0011815B2F", "query_cov": 1.0427350427350428, "subject_cov": 0.8413793103448276, "identity": 0.617, "score": 389.0, "evalue": 1.61e-130, "valid": false, "product": "ABC transporter permease", "uniref50_id": "UniRef50_UPI0011815B2F" }, "pscc": { "uniref50_id": "UniRef50_UPI0011815B2F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0011815B2F" ], "product": "ABC transporter permease" }, "genes": [], "id": "KMADJIBOEG_193", "locus": "GCA000292445_00154" }, { "type": "cds", "sequence": "contig_1", "start": 77678, "stop": 77878, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTTTTCTCTTTAAACAAAATCATAAAACCCAACCAGAAATTCACTAGTAATGAAGCTGAATTTCTACAGATAGCTACTGATTATTTGGAGGAAAGTCAAAACTATCTTCAAAAGGGTTTAAAGCAATTAAAAAAAGAATATAAAAGATCCATTATTTATAACCCTAACCTTGAATATAAACGCTTTGTTAAATGA", "aa": "MDFFSLNKIIKPNQKFTSNEAEFLQIATDYLEESQNYLQKGLKQLKKEYKRSIIYNPNLEYKRFVK", "aa_hexdigest": "98c702ed2232c26b335f4205013eaed0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8026.137799999998, "isoelectric_point": 9.397765922546387 }, "id": "KMADJIBOEG_194", "locus": "GCA000292445_00155" }, { "type": "cds", "sequence": "contig_1", "start": 78389, "stop": 79072, "strand": "+", "frame": 2, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P9E0" ], "nt": "GTGTATAAATACATCACTAATGGCATTACTACAAATGCAGTTCTTAAAGGAGTTGATCTTGCCATTAAAAGTCATGATTTTATTGTGATTTTAGGCCCTTCAGGATCTGGTAAAACCACATTACTAAACATTATTTCAGGGATGGATAGAGCTTCTAGTGGTAGTGTTATTGTCAATGGTTATAACATGATTTGTTTAAATGATAGAAAGCTCACTAAATTCCGTCAAAAGTATGTTGGTTACATCTTTCAACAATACGGTTTATTACCTAATTTAACAGTTAGAGAAAACATTGAGATAGGAGCAAATCTTCAACCAGATCCTAGTAAAAGGATCAGCATTGATGCACTTTTAGAAGCGGTTGGGATGGATAGTTTGCAAAAGAAGCTTCCTAATGAATTGAGTGGTGGGCAACAGCAACGTGTTTCCATTGCAAGAGCTTTTGCTAAAAACCCCTTATTAATTTTTGGTGATGAACCTACTGGGGCACTTGATCTTGAGATGACCCAAATTGTTTTAAAACAGTTTTTAGCAATTAAAAAGCGTTATCAAACGACAATGATTATTGTTACCCACAACAATTTAATTGCTAACTTAGCTGATTTAGTTATCTATGTAGCAGATGGAAAAATAAAATCACTACACAGAAACTTAAATCCTAAACAGGTTGAAGAGATCCATTGA", "aa": "MYKYITNGITTNAVLKGVDLAIKSHDFIVILGPSGSGKTTLLNIISGMDRASSGSVIVNGYNMICLNDRKLTKFRQKYVGYIFQQYGLLPNLTVRENIEIGANLQPDPSKRISIDALLEAVGMDSLQKKLPNELSGGQQQRVSIARAFAKNPLLIFGDEPTGALDLEMTQIVLKQFLAIKKRYQTTMIIVTHNNLIANLADLVIYVADGKIKSLHRNLNPKQVEEIH", "aa_hexdigest": "bc7686ff677e6c1249c40cc7f03caf28", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P9E0", "query_cov": 0.8766519823788547, "subject_cov": 0.9900497512437811, "identity": 0.698, "score": 267.0, "evalue": 3.76e-88, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A3B0P9E0" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P9E0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P9E0" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "KMADJIBOEG_195", "locus": "GCA000292445_00156" }, { "type": "cds", "sequence": "contig_1", "start": 79082, "stop": 79933, "strand": "+", "frame": 2, "gene": "tkt", "product": "transketolase", "db_xrefs": [ "COG:COG0021", "COG:G", "EC:2.2.1.1", "GO:0004802", "GO:0005829", "GO:0006098", "GO:0046872", "KEGG:K00615", "SO:0001217", "UniRef:UniRef50_P75611", "UniRef:UniRef90_P47312" ], "nt": "ATGAAATACTTGTATGCCACTCAACACCTTACTTTAAATGCTATTAAGCATGCTAAGGGAGGACATGTTGGCATGGCCATTGGTGCAAGTCCTATCTTATTTAGTTTATTTACTAAACACTTTCACTTTGATCCTGACCAACCAAAGTGGATCAACAGAGATCGCTTTGTTTTAAGTGCTGGCCATGGTAGCATGGCATTATATTCAATTTTCCATTTTGCCGGACTTATTTCTAAACAAGAGATCTTACAGCATAAACATGGTCAAATTAACACTTCTTCCCATCCTGAATATGCTCCAAATAACTTCATAGATGCATCAACAGGCCCTTTAGGTCAAGGCTTTGGCATGGCAGTTGGCATGGCGTTAGCACAAAAGTTATTAGCTAATGAATTTAAAGAGCTAAGTGATAAATTGTTTGACCATTACACCTATGTGGTTGTTGGGGATGGAGATCTACAGGAGGGGGTTAGTTATGAAGTTAGTCAAATTGCTGGGTTATATAAATTAAATAAACTAATTGTGCTTCATGATTCAAATAGAGTGCAAATGGATAGTGAAGTAAAAAAAGTTGCTAATGAAAATCTAAAGGTTAGGTTTGAAAACGTTGGTTGGAATTACATCCATACTGATGATCAACTAGAAAATATTGATCAAGCTATTATTAAAGCCAAACAATCAGATAAGCCAACTTTTATTGAAGTGAGAACAACTATTGCTAAAAACACCCACCTTGAAGATCAGTATGGAGGACATTGGTTTATTCCCAATGAAGTTGACTTTCAACTTTTTGAGAAAAGAACAAATACTAACTTTAACTTTTTTAATTATCCAGATAGTATTTACCAATGA", "aa": "MKYLYATQHLTLNAIKHAKGGHVGMAIGASPILFSLFTKHFHFDPDQPKWINRDRFVLSAGHGSMALYSIFHFAGLISKQEILQHKHGQINTSSHPEYAPNNFIDASTGPLGQGFGMAVGMALAQKLLANEFKELSDKLFDHYTYVVVGDGDLQEGVSYEVSQIAGLYKLNKLIVLHDSNRVQMDSEVKKVANENLKVRFENVGWNYIHTDDQLENIDQAIIKAKQSDKPTFIEVRTTIAKNTHLEDQYGGHWFIPNEVDFQLFEKRTNTNFNFFNYPDSIYQ", "aa_hexdigest": "c9eaae64a7b9f4d7e3665cfe1b198bca", "start_type": "ATG", "rbs_motif": null, "genes": [ "tkt" ], "truncated": "3-prime", "pseudogene": { "start": 79082, "stop": 80236, "observations": { "mutation": [ 79931 ], "stop": [ 79931 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47312", "query_cov": 0.9964664310954063, "subject_cov": 0.4351851851851852, "identity": 0.996, "score": 577.0, "evalue": 1.33e-202, "gene_start": 1, "gene_end": 282, "reference_start": 1, "reference_end": 282, "reference_sequence": "MKYLYATQHLTLNAIKHAKGGHVGMAIGASPILFSLFTKHFHFDPDQPKWINRDRFVLSAGHGSMALYSIFHFAGLISKQEILQHKHGQINTSSHPEYAPNNFIDASTGPLGQGFGMAVGMVLAQKLLANEFKELSDKLFDHYTYVVVGDGDLQEGVSYEVSQIAGLYKLNKLIVLHDSNRVQMDSEVKKVANENLKVRFENVGWNYIHTDDQLENIDQAIIKAKQSDKPTFIEVRTTIAKNTHLEDQYGGHWFIPNEVDFQLFEKRTNTNFNFFNYPDSIYHWFKQTVIERQKQIKEDYNNLLISLKDKPLFKKFTNWIDSDFQALYLNQLDEKKVAKKDSATRNYLKDFLNQINNPNSNLYCLNADVSRSCFIKIGDDNLHENPCSRNIQIGIREFAMATIMNGMALHGGIKVMGGTFLAFADYSKPAIRLGALMNLPVFYVYTHDSYQVGGDGPTHQPYDQLPMLRAIENVCVFRPCDEKETCAGFNYGLLSQDQTTVLVLTRQPLKSIDNTDSLKTLKGGYILLDRKQPDLIIAASGSEVQLAIEFEKVLTKQNVKVRILSVPNITLLLKQDEKYLKSLFDANSSLITIEASSSYEWFCFKKYVKNHAHLGAFSFGESDDGDKVYQQKGFNLERLMKIFTSLRN" }, "paralog": false, "identity": 0.9921875, "score": 780.0, "evalue": 4.26e-285, "description": "Internal stop codon at 79931. Nonsense mutation around 79931.", "psc": { "uniref90_id": "UniRef90_P47312", "gene": "tkt", "product": "transketolase", "ec_ids": [ "2.2.1.1" ], "uniref50_id": "UniRef50_P75611", "cog_id": "COG0021", "cog_category": "G", "kegg_orthology_id": "K00615", "go_ids": [ "GO:0005829", "GO:0046872", "GO:0004802", "GO:0006098" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47312", "EC:2.2.1.1", "UniRef:UniRef50_P75611", "COG:COG0021", "COG:G", "KEGG:K00615", "GO:0005829", "GO:0046872", "GO:0004802", "GO:0006098" ] }, "pscc": { "uniref50_id": "UniRef50_P75611", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75611" ], "product": "Transketolase" } }, "id": "KMADJIBOEG_196", "locus": "GCA000292445_00157" }, { "type": "cds", "sequence": "contig_1", "start": 79988, "stop": 81028, "strand": "+", "frame": 2, "gene": "tkt", "product": "transketolase", "db_xrefs": [ "COG:COG0021", "COG:G", "EC:2.2.1.1", "GO:0004802", "GO:0005829", "GO:0006098", "GO:0046872", "KEGG:K00615", "SO:0001217", "UniRef:UniRef50_P75611", "UniRef:UniRef90_P47312" ], "nt": "TTGCTAATTTCTCTTAAAGACAAACCACTTTTTAAAAAATTTACTAATTGGATTGACAGTGATTTTCAAGCCCTTTATCTTAACCAACTAGATGAAAAGAAAGTAGCAAAAAAAGATAGTGCTACTAGAAACTATTTAAAAGATTTTTTAAACCAAATTAATAATCCTAATTCCAACTTGTATTGCTTAAATGCTGATGTATCACGTTCTTGTTTTATCAAGATAGGTGATGATAATCTCCATGAAAATCCTTGTTCTAGAAATATCCAAATAGGAATTAGGGAGTTTGCAATGGCAACAATAATGAATGGTATGGCACTTCATGGTGGTATTAAAGTGATGGGTGGTACTTTTTTAGCATTTGCTGATTATTCAAAGCCAGCAATTCGCTTAGGTGCATTAATGAACTTACCAGTATTTTATGTTTATACCCATGACTCTTATCAAGTAGGGGGTGATGGTCCTACTCATCAACCCTATGATCAACTACCAATGTTAAGAGCAATTGAAAATGTTTGTGTATTTCGTCCTTGTGATGAAAAGGAAACTTGTGCTGGATTTAACTATGGTCTTTTAAGTCAAGATCAGACAACTGTTTTGGTTTTAACACGTCAACCCTTAAAATCAATTGATAACACTGATAGTTTAAAAACACTGAAAGGTGGCTATATCCTTTTGGATAGAAAACAACCTGATTTAATTATTGCTGCTAGTGGTAGTGAAGTGCAACTTGCAATAGAGTTTGAAAAAGTTTTAACTAAACAAAATGTAAAGGTAAGAGTTCTGTCAGTTCCCAATATAACTTTGCTTTTAAAACAAGATGAAAAATATCTAAAGAGTTTATTTGATGCTAACAGTTCACTTATCACTATAGAAGCGAGTAGTAGCTATGAATGGTTTTGCTTTAAAAAGTATGTTAAAAACCATGCTCACTTAGGAGCTTTTAGTTTTGGTGAATCTGATGATGGAGATAAAGTTTATCAGCAAAAAGGGTTTAATCTGGAAAGGTTAATGAAAATATTTACTTCCCTAAGAAATTAA", "aa": "MLISLKDKPLFKKFTNWIDSDFQALYLNQLDEKKVAKKDSATRNYLKDFLNQINNPNSNLYCLNADVSRSCFIKIGDDNLHENPCSRNIQIGIREFAMATIMNGMALHGGIKVMGGTFLAFADYSKPAIRLGALMNLPVFYVYTHDSYQVGGDGPTHQPYDQLPMLRAIENVCVFRPCDEKETCAGFNYGLLSQDQTTVLVLTRQPLKSIDNTDSLKTLKGGYILLDRKQPDLIIAASGSEVQLAIEFEKVLTKQNVKVRVLSVPNITLLLKQDEKYLKSLFDANSSLITIEASSSYEWFCFKKYVKNHAHLGAFSFGESDDGDKVYQQKGFNLERLMKIFTSLRN", "aa_hexdigest": "ad9eea3605d7a38fc6d375c1bf67d9c0", "start_type": "TTG", "rbs_motif": null, "genes": [ "tkt" ], "truncated": "5-prime", "pseudogene": { "start": 79688, "stop": 81028, "observations": { "mutation": [ 79931 ], "stop": [ 79931 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47312", "query_cov": 1.0, "subject_cov": 0.5339506172839507, "identity": 0.9940000000000001, "score": 699.0, "evalue": 1.22e-249, "gene_start": 1, "gene_end": 346, "reference_start": 303, "reference_end": 648, "reference_sequence": "MKYLYATQHLTLNAIKHAKGGHVGMAIGASPILFSLFTKHFHFDPDQPKWINRDRFVLSAGHGSMALYSIFHFAGLISKQEILQHKHGQINTSSHPEYAPNNFIDASTGPLGQGFGMAVGMVLAQKLLANEFKELSDKLFDHYTYVVVGDGDLQEGVSYEVSQIAGLYKLNKLIVLHDSNRVQMDSEVKKVANENLKVRFENVGWNYIHTDDQLENIDQAIIKAKQSDKPTFIEVRTTIAKNTHLEDQYGGHWFIPNEVDFQLFEKRTNTNFNFFNYPDSIYHWFKQTVIERQKQIKEDYNNLLISLKDKPLFKKFTNWIDSDFQALYLNQLDEKKVAKKDSATRNYLKDFLNQINNPNSNLYCLNADVSRSCFIKIGDDNLHENPCSRNIQIGIREFAMATIMNGMALHGGIKVMGGTFLAFADYSKPAIRLGALMNLPVFYVYTHDSYQVGGDGPTHQPYDQLPMLRAIENVCVFRPCDEKETCAGFNYGLLSQDQTTVLVLTRQPLKSIDNTDSLKTLKGGYILLDRKQPDLIIAASGSEVQLAIEFEKVLTKQNVKVRILSVPNITLLLKQDEKYLKSLFDANSSLITIEASSSYEWFCFKKYVKNHAHLGAFSFGESDDGDKVYQQKGFNLERLMKIFTSLRN" }, "paralog": false, "identity": 0.9932735426008968, "score": 902.0, "evalue": 0.0, "description": "Internal stop codon at 79931. Nonsense mutation around 79931.", "psc": { "uniref90_id": "UniRef90_P47312", "gene": "tkt", "product": "transketolase", "ec_ids": [ "2.2.1.1" ], "uniref50_id": "UniRef50_P75611", "cog_id": "COG0021", "cog_category": "G", "kegg_orthology_id": "K00615", "go_ids": [ "GO:0005829", "GO:0046872", "GO:0004802", "GO:0006098" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47312", "EC:2.2.1.1", "UniRef:UniRef50_P75611", "COG:COG0021", "COG:G", "KEGG:K00615", "GO:0005829", "GO:0046872", "GO:0004802", "GO:0006098" ] }, "pscc": { "uniref50_id": "UniRef50_P75611", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75611" ], "product": "Transketolase" } }, "id": "KMADJIBOEG_197", "locus": "GCA000292445_00158" }, { "type": "cds", "sequence": "contig_1", "start": 81078, "stop": 81287, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "ATGCCTGGTTGCATCTTACTTTCATCCTGTTCTTTTAGAAGTTATATCCCAACTCCTAGTTTAAGAAATACTGTTGGTAACCACAACAGTTATGTTAATAATACTGTCCCTAAAAACAATTTTTATGAAAAGTTTTATGATCTAACTTTTGCTTTAAATTTCACTAATCAGAAAACTCAAGAGTTTGGTACTGGTTGGTTAATTGACTGA", "aa": "MPGCILLSSCSFRSYIPTPSLRNTVGNHNSYVNNTVPKNNFYEKFYDLTFALNFTNQKTQEFGTGWLID", "aa_hexdigest": "fc002f78baf29bb0d383dafd8541c01e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 39, "aa_cov": 0.4492753623188406, "hmm_cov": 1.0, "evalue": 4.979696176361972e-08, "score": 28.07671546936035, "start": 39, "stop": 69 } ], "seq_stats": { "molecular_weight": 7916.801200000002, "isoelectric_point": 7.786185264587402 }, "id": "KMADJIBOEG_198", "locus": "GCA000292445_00159" }, { "type": "cds", "sequence": "contig_1", "start": 81423, "stop": 81707, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "GTGATAGACGGTTTAAAGAATGATCATGATTATCAACCATATAACAAAGATGGAAATGGTCTTAGTTTTCCATTTGATCAAAAAACCCAATCATTCTTATTGGGTAGGTTTGCCAATCCTAAGATAAATTCCAAACCAGAAGAGATGAACTACCAAGTTCAAACTCGTTTAAAACAAGATGCAATGGTGTTTATCCAAACCAGTACTTTACCTAAAACTGCTTATGCAGGAATTGATCCTATTAACTTTGATTACCATGAAACTAGTGATGAGAGTGGATTTTGA", "aa": "MIDGLKNDHDYQPYNKDGNGLSFPFDQKTQSFLLGRFANPKINSKPEEMNYQVQTRLKQDAMVFIQTSTLPKTAYAGIDPINFDYHETSDESGF", "aa_hexdigest": "4923a12b01435c1ad92eba6f14e48df8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 89, "aa_cov": 0.9148936170212766, "hmm_cov": 0.898876404494382, "evalue": 1.8984052914909494e-10, "score": 36.030906677246094, "start": 2, "stop": 87 } ], "seq_stats": { "molecular_weight": 10770.800599999993, "isoelectric_point": 4.909604454040527 }, "id": "KMADJIBOEG_199", "locus": "GCA000292445_00160" }, { "type": "cds", "sequence": "contig_1", "start": 82613, "stop": 84037, "strand": "+", "frame": 2, "gene": null, "product": "putative lipoprotein MG068", "db_xrefs": [ "GO:0005886", "RefSeq:WP_014894130.1", "SO:0001217", "UniParc:UPI00027B3768", "UniRef:UniRef100_UPI00027B3768", "UniRef:UniRef50_P47314", "UniRef:UniRef90_P47314" ], "nt": "GTGTTAAGACGTTATCTAACTCTGAGTTTTTCTAGCTTGCTTTTATTAGCACTGCTGTTTTTAACAGGCTGTTCTTTTGTTAGACCACAATTTCGCAGGGGCTTTAGAACCCAGTTTAAAATTAACTCTATTCCAACTGTTAGTGATCCTTATCACATTAACTACGACTTAACTTTTTCATTAAACTTTGCTTCCAACAAAAGAAATACTTATGGTACTGGTTGGTTAATTGATTGGAAAGGAGATGAAAATAACCCTGAGAAAAATGATCCTTTTAAAGTTTATTTAGCTACTAACCTCCATGTGATAGATGCGTTAAGAAATAATAATGACTATGAACCATATAACAAGGATAGTAATAGTCAAGCTTTTAATGGCGAAGAGATCACTAGGTTCTTTTCTATAGGTAAGTACACATATCCTAGTATTTTTAGTGAATTAAATTTCATTAGCAATGCTAGAGAAGCGTTTGTTTCGATTCAAACCTCTACTATTCCTAAAACTGCTTATGCTGCTGTTAACTTTGTTGAAACACAAGGAGAGGATGAAAGTTATACAGATTCATTATCAACTGATAATAAAAGAGATATTTATGCGGATTTTGCTGTGATAGAAATCCCCTTATTCTTAACTAACCATCGCGATTATCAAGTATTTAATGAATTTATTAAACCAGCAATAGAAACATACAAACAACTAGGAAACTCTCCCTTTGAAGAAAAACAACTAGATCAGCATAAAAACGACAACTTTTACATGTTAGGTTACCCTTTGGTTGAAAGTAGCATAGATGCTCTAATTTTAAACCAAAGAAGACAATACAATAACAGTTATACTGAAAAATATACTCCTCAAACTTTGACCAAAGACCAACGCACTATTGACTTAAGCAGAGAAGTTCCTACTTTAATTCAGAACAAAACAGAAAATTCTACAGGAAGTCAATTACTAGTAAATCAATCTTTAAGTAGTACATCTGAAGGGATTATTGAATTTATTAAGTTACCTGAATTTAAACTCAATTATCATAATAAAAGTTACCGTCAATATGGTAGAGGTTTTGCTCTACAAAACACTAACTTTAGACCAGGTTCTTCAGGAACTTTAATGTTAAATAACCAAAAACAGATAGCAGGTATTTATTTTGGTGTTTTAGATTTTGGAGAAGATGTAAGTTTAATGAGTAACATTGGCGTTGGACAAATTCTTCGTGTTCCTCAAAAGAACAATACTAGAAATAGATCAATTGCTACTAATAAAAGCAACTATGATTTGATCTTTGGTGATAGCAATACAACTAACTTTTATGCAAAATTTGCTAGACAAAATAACACCCATCTTTATCAGATGATTTCAAATAGTAAAGATACAAAATTAAAGTATGTGAATACTGTTGAAAAAACAGTAAAAGCTAGTATTAAATAA", "aa": "MLRRYLTLSFSSLLLLALLFLTGCSFVRPQFRRGFRTQFKINSIPTVSDPYHINYDLTFSLNFASNKRNTYGTGWLIDWKGDENNPEKNDPFKVYLATNLHVIDALRNNNDYEPYNKDSNSQAFNGEEITRFFSIGKYTYPSIFSELNFISNAREAFVSIQTSTIPKTAYAAVNFVETQGEDESYTDSLSTDNKRDIYADFAVIEIPLFLTNHRDYQVFNEFIKPAIETYKQLGNSPFEEKQLDQHKNDNFYMLGYPLVESSIDALILNQRRQYNNSYTEKYTPQTLTKDQRTIDLSREVPTLIQNKTENSTGSQLLVNQSLSSTSEGIIEFIKLPEFKLNYHNKSYRQYGRGFALQNTNFRPGSSGTLMLNNQKQIAGIYFGVLDFGEDVSLMSNIGVGQILRVPQKNNTRNRSIATNKSNYDLIFGDSNTTNFYAKFARQNNTHLYQMISNSKDTKLKYVNTVEKTVKASIK", "aa_hexdigest": "0222747a7555cd9bda7f578ba53110c6", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3768", "ncbi_nrp_id": "WP_014894130.1", "uniref100_id": "UniRef100_UPI00027B3768", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3768", "RefSeq:WP_014894130.1", "UniRef:UniRef100_UPI00027B3768" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3768", "uniref90_id": "UniRef90_P47314" }, "psc": { "uniref90_id": "UniRef90_P47314", "product": "Uncharacterized lipoprotein MG068", "uniref50_id": "UniRef50_P47314", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47314", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47314" ], "product": "Uncharacterized lipoprotein MG068" }, "genes": [], "id": "KMADJIBOEG_200", "locus": "GCA000292445_00161" }, { "type": "cds", "sequence": "contig_1", "start": 84075, "stop": 84338, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_014894131.1", "SO:0001217", "UniParc:UPI00027B3769", "UniRef:UniRef100_UPI00027B3769", "UniRef:UniRef50_UPI00027B3523", "UniRef:UniRef90_UPI00027B3769" ], "nt": "ATGGAAAATCAAAACAGTTGTAGTTCTGTAAAAGAAAAAGAGTTATATAATCCAAAAAAGCCTTTTCTTTTTGCGGTAATTTCTTTCTTTGTTTTCATAATATTTTTTGCTTTAGCAGGTTCATTTACCAAATTTGCATTAGAAGAAAGCAGGGGTTATAGTTTTCTTGTTATTGTCTGTTATCTTCTAGGAACAGTTTGTTTTTTGTTTTTATCTATTTTTGGTTTTGATTCTTATCTGAATTTTTTGCAAATAGAGTTCTAG", "aa": "MENQNSCSSVKEKELYNPKKPFLFAVISFFVFIIFFALAGSFTKFALEESRGYSFLVIVCYLLGTVCFLFLSIFGFDSYLNFLQIEF", "aa_hexdigest": "7f5afd7940d19751bfbc63f75d3befb4", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3769", "ncbi_nrp_id": "WP_014894131.1", "uniref100_id": "UniRef100_UPI00027B3769", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3769", "RefSeq:WP_014894131.1", "UniRef:UniRef100_UPI00027B3769" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3769", "uniref90_id": "UniRef90_UPI00027B3769" }, "psc": { "uniref90_id": "UniRef90_UPI00027B3769", "uniref50_id": "UniRef50_UPI00027B3523" }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3523", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3523" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10076.71689999999, "isoelectric_point": 5.188911247253416 }, "id": "KMADJIBOEG_201", "locus": "GCA000292445_00162" }, { "type": "cds", "sequence": "contig_1", "start": 84524, "stop": 84736, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTATTTGGTAATCCTTGTTTAGTAGATCCTAATAAAATGAAATTAAATGCTAATACACTAACAACAACTAAGAGTGTTATTAATATTGATCACAACAAAATTAAAGTTGTCGTTAGAAATTTAACTTTGCTTGTTTCTATTTCATTTAAACAAGTCTTTTTATCATTTGAAATATTTAATTCTTTTTGCACTTTCCAACTAATAAAATAA", "aa": "MVFGNPCLVDPNKMKLNANTLTTTKSVINIDHNKIKVVVRNLTLLVSISFKQVFLSFEIFNSFCTFQLIK", "aa_hexdigest": "5323dbaff72f7840aa10d85d3e23a558", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7977.432699999995, "isoelectric_point": 9.698317527770996 }, "id": "KMADJIBOEG_202", "locus": "GCA000292445_00163" }, { "type": "cds", "sequence": "contig_1", "start": 84768, "stop": 85043, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAATTATCACAAAATGGTGTGATCTTATCAATAGCACTACTTGCTTTTTTTAGTTGGATGGCAATTGTTGGAATTCACAGCTTTTTAGTTGGAATTTTAGCTAATCATCAAACACTTAAAATAAGTAAAAAGTATGTTATTTTAGGTTCGATTTTTCCAATTATGGCATTAACAAATACTCTTGTAATTAGAAAAAAATTAAAAGCTTTATTAGGAGAGGGTAAGGTTCAAAAAGGACTCAAAGTTTTAACTATTAGTTTTATTTGTGTTTGA", "aa": "MQLSQNGVILSIALLAFFSWMAIVGIHSFLVGILANHQTLKISKKYVILGSIFPIMALTNTLVIRKKLKALLGEGKVQKGLKVLTISFICV", "aa_hexdigest": "769ecc93991720779e2d3da3d34c8d07", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9953.15349999999, "isoelectric_point": 10.37175426483154 }, "id": "KMADJIBOEG_203", "locus": "GCA000292445_00164" }, { "type": "cds", "sequence": "contig_1", "start": 85311, "stop": 85547, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED97BE8", "UniRef:UniRef90_UPI001ED97BE8" ], "nt": "TTGGCTTTAACCTTAATTGATTTAATGGGTTTAATTTTGGCTTTTGGTATTATTGTTGTTAACAATACAAATTCTTCAAGTTTTGATGCAAAATCAAATTCCTTAGCATTAATTTTTAAGTTCATTTATTTTGATAGTTCTATAGCAGCCTTTTTAACAGTTTTAAGCTTTGCTAGTTCAGTTGCTTTACTTGTCAATTTAATAAAAAAAGATAAGGGATTAACCTTAAAAAATTAG", "aa": "MALTLIDLMGLILAFGIIVVNNTNSSSFDAKSNSLALIFKFIYFDSSIAAFLTVLSFASSVALLVNLIKKDKGLTLKN", "aa_hexdigest": "bcd56ee307a3d42b1e867789222c4fdd", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 85047, "stop": 85547, "observations": { "mutation": [ 85074, 85218, 85284 ], "stop": [ 85074, 85218, 85284 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001ED97BE8", "query_cov": 1.0, "subject_cov": 0.46987951807228917, "identity": 0.9620000000000001, "score": 130.0, "evalue": 4.56e-37, "gene_start": 1, "gene_end": 78, "reference_start": 89, "reference_end": 166, "reference_sequence": "MQLIIGFFVWLFPYAGEAGINIGINLSIFNLAQLVGSDINVITYFTTFLTLLFAFLSWAVLHVLACFILVHLTIYKQQEWIKIKAIYILALTLIDLMGLILAFGIIVVNNTNSSSVDAKSNSLALIFKFIYFGSSIAAFLTVLSFASSVALLVNLIKKDKGLTLKN" }, "paralog": false, "identity": 0.9518072289156626, "score": 293.0, "evalue": 8.7e-104, "description": "Internal stop codon at 85074, 85218, 85284. Nonsense mutation around 85074, 85218, 85284.", "psc": { "uniref90_id": "UniRef90_UPI001ED97BE8", "uniref50_id": "UniRef50_UPI001ED97BE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001ED97BE8", "UniRef:UniRef50_UPI001ED97BE8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001ED97BE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED97BE8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8387.883999999991, "isoelectric_point": 9.224861717224119 }, "id": "KMADJIBOEG_204", "locus": "GCA000292445_00165" }, { "type": "cds", "sequence": "contig_1", "start": 85572, "stop": 85931, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTACTAGTGCCAAAAGTGGTATTGGAGTATCACAACCTGAATAACCAAGTAGTCAAAGAGAGTTTGGAAGTGGAATCTTCTTCCTCCTTTAACCCCACCCAAAGGTTGAAAAAAGATAGTCCAATGAAGGATTCAAGTAAACACAGGGAGAAACTCACCGAAACAACTTCATCGATGAGTAGTGGTGGGGCTACATCCACTCGAGCCAAGGCCCTCAAGGTTGAGGTGGAAAGGGGGAGTCAAAGTGATTCACTTTTAAAAAACGACTTTGCCAAAAAGCCACTGAAACATAAAGAAAATAGTGGGACAGATGTGAAGTTGGATGCGAGTGGGGAGTTTGCCAATGATAAAGCCTGA", "aa": "MLLVPKVVLEYHNLNNQVVKESLEVESSSSFNPTQRLKKDSPMKDSSKHREKLTETTSSMSSGGATSTRAKALKVEVERGSQSDSLLKNDFAKKPLKHKENSGTDVKLDASGEFANDKA", "aa_hexdigest": "fbf642def48db0abac5f65c333c88dd2", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030CD7D5B", "query_cov": 0.9327731092436975, "subject_cov": 0.8409090909090909, "identity": 0.838, "score": 167.0, "evalue": 2.13e-51, "valid": false, "product": "adhesin", "uniref50_id": "UniRef50_A0A7M3VDG5" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13079.524499999981, "isoelectric_point": 9.126031684875489 }, "id": "KMADJIBOEG_205", "locus": "GCA000292445_00166" }, { "type": "cds", "sequence": "contig_1", "start": 85945, "stop": 86088, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGAAAAAGTGGGGAGTGGGGTAGAAGAGGAAGTAGAAGTAGAAGAAGAAGGGGAGTTGGAGAAGGGTTTACCATTGCCGATTCAGGGGAGAAAGTCTAAGTCGCCCCCATCCCCTTCTCTCTTGCTATTTGCTCGGTAG", "aa": "MLEKVGSGVEEEVEVEEEGELEKGLPLPIQGRKSKSPPSPSLLLFAR", "aa_hexdigest": "2ad57c68ac8a3711e38ac8b8fa6a184b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5119.796600000001, "isoelectric_point": 4.638994026184082 }, "id": "KMADJIBOEG_206", "locus": "GCA000292445_00167" }, { "type": "cds", "sequence": "contig_1", "start": 86281, "stop": 86595, "strand": "+", "frame": 1, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49206", "UniRef:UniRef90_UPI0030D20171" ], "nt": "ATGATAATAAAGTCACACCATCAGGTGGCTCCCCCAAAAAAAACCACCTATCCTTTTTTACCCAACAGTATCAGTCCCACCAGTGACTGGATCAATGCGTTGACTTTCACTAATAAGAATAACCCGCAACGAAATCAGTTGTTGCTCAGAAGCTTACTAGGAACGATCCCGGTATTGATCAATAAGAGTGGAGGACGTGGGGATGAGTTTACCCATACGAGTGATCAGAAGTGGGATAAAACTGAGACAAATGAGGGCAACCTCCCGGGGTTTGGGGAGGTGAATGGCGGTTTTTACCCAGTTTTACTTATTTAA", "aa": "MIIKSHHQVAPPKKTTYPFLPNSISPTSDWINALTFTNKNNPQRNQLLLRSLLGTIPVLINKSGGRGDEFTHTSDQKWDKTETNEGNLPGFGEVNGGFYPVLLI", "aa_hexdigest": "ea47f6b2cb0cb6149f3bb3e0df7c5ca7", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 86244, "stop": 86595, "observations": { "insertion": [ 86313 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0030D20171", "query_cov": 0.8846153846153846, "subject_cov": 0.7796610169491526, "identity": 0.924, "score": 179.0, "evalue": 1.41e-56, "gene_start": 13, "gene_end": 104, "reference_start": 27, "reference_end": 118, "reference_sequence": "MKPSSNNNQQFNPNSDDNKVTSGGSSKPTTYTHLPNSISPTSDWINALTFTNKNNPQRNQLLLRGLLGTIPVLINKSGGSGNEFNHTSDQKWDKTETNEGNLPGFGEVNGGFYPVLLI" }, "paralog": false, "identity": 0.8888888888888888, "score": 203.0, "evalue": 9.67e-69, "description": "Frameshift due to insertion around 86313.", "psc": { "uniref90_id": "UniRef90_UPI0030D20171", "product": "adhesin", "uniref50_id": "UniRef50_Q49206", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0030D20171", "UniRef:UniRef50_Q49206" ] }, "pscc": { "uniref50_id": "UniRef50_Q49206", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49206" ], "product": "Random genomic clone esh10, partial cds" } }, "id": "KMADJIBOEG_207", "locus": "GCA000292445_00168" }, { "type": "cds", "sequence": "contig_1", "start": 87016, "stop": 87192, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13", "UniRef:UniRef90_UPI0030D43D23" ], "nt": "GTGGTGAAAAAGTTTAAGTTAGATGTGCCAAGTGTTTCAACAACCATGAACGAAGTGTTGCAAAACCTCATCCTTGAACAACCTTTGACTAAGTATACGCTTAATAGTAGTTTGGCTAAACAAAAGGGCAAAAGCCAGATAGAGGTGCATCTGGGTAGTGGGCAAGCAAATAATTGA", "aa": "MVKKFKLDVPSVSTTMNEVLQNLILEQPLTKYTLNSSLAKQKGKSQIEVHLGSGQANN", "aa_hexdigest": "df96736ade49c3b023490562a405e82d", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 86887, "stop": 87257, "observations": { "deletion": [ 87214 ], "mutation": [ 87010, 87190 ], "stop": [ 87010, 87190 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0030D43D23", "query_cov": 0.9655172413793104, "subject_cov": 0.4628099173553719, "identity": 0.821, "score": 93.6, "evalue": 2.1e-23, "gene_start": 1, "gene_end": 56, "reference_start": 42, "reference_end": 97, "reference_sequence": "MKLTYSSSGSNTNTISFENNANKPSYVVEFTNSTNVGIKWRVVKKYQLDVPNVSSDMNQVLQELILEQPLTKYTLNSSLAKQKGKSKIEVHLGSGRATQWTSRTQLHWPKQQSQPKCINRI" }, "paralog": false, "identity": 0.7235772357723578, "score": 166.0, "evalue": 7.86e-55, "description": "Internal stop codon at 87010, 87190. Frameshift due to deletion around 87214. Nonsense mutation around 87010, 87190.", "psc": { "uniref90_id": "UniRef90_UPI0030D43D23", "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0030D43D23", "UniRef:UniRef50_UPI00027B3D13" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6400.338600000001, "isoelectric_point": 9.523801612854005 }, "id": "KMADJIBOEG_208", "locus": "GCA000292445_00169" }, { "type": "cds", "sequence": "contig_1", "start": 87199, "stop": 87300, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTAATCAACACAACCTAAACAACAATCCCTCTCCCAATGCATCAACCGGATTTAAATTAAATAAAGGCAACGCATATAGAAAACTAAGTGAATCCTGA", "aa": "MRNQHNLNNNPSPNASTGFKLNKGNAYRKLSES", "aa_hexdigest": "40538a33f0ce3d3863633d24090f39e8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3703.0246000000006, "isoelectric_point": 10.446408653259276 }, "id": "KMADJIBOEG_209", "locus": "GCA000292445_00170" }, { "type": "cds", "sequence": "contig_1", "start": 87320, "stop": 87433, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGACCAAGCACGGCAAAGGGAAGGATAGTAGTGGGTGGAATAGTGAAGAAAATACGGCAGCAGGGGATGCGCCCAGTGTTACTGGAGGGAGGAGGTGGGGGATCAAATTAA", "aa": "MGPSTAKGRIVVGGIVKKIRQQGMRPVLLEGGGGGSN", "aa_hexdigest": "c706b5ab7414e3399dd7596c904a27f6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3706.3481, "isoelectric_point": 11.733906745910645 }, "id": "KMADJIBOEG_210", "locus": "GCA000292445_00171" }, { "type": "cds", "sequence": "contig_1", "start": 88300, "stop": 88608, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCCCTATTGCAGTTTTGCCTTTAGCAGGTATTTTTTTAGGAATCGGTGATGCCATTTCTTCCAATTCATCTGGCATTGTTGGTGTGAAATTTTTTGGTGAATTTATTAAACAAGGTGGTAATGTAGTTTTTGCTAACTTACCTATTTTGTTTGCAGTTGCAATTGCGATCACCTTTTCTCAAGATGCAGGGGTTGCTGGATTTTCTGCTTTTGTTTTTTGGGCCACAATGAACGCGTTTATGAGTTCATTAATTATTCCTGTTGATGCAAATAATACTGCTTCAGGTTATAACATCCTTTATTGA", "aa": "MIPIAVLPLAGIFLGIGDAISSNSSGIVGVKFFGEFIKQGGNVVFANLPILFAVAIAITFSQDAGVAGFSAFVFWATMNAFMSSLIIPVDANNTASGYNILY", "aa_hexdigest": "c509c2615c2ebfb2fb4f53bc0cc62f8f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10582.222899999993, "isoelectric_point": 4.225490379333496 }, "id": "KMADJIBOEG_211", "locus": "GCA000292445_00172" }, { "type": "cds", "sequence": "contig_1", "start": 89055, "stop": 89312, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGAACCATCTAATAAACCGTTTTTAGCCAATTCATCAAAGTTACCTTTACGTGTAAGACCATCAACAAGTTCTTTTCAACTTAGAAAGTTTGTTGATTCTTTACTCATGTATTTTGAAAGTGCAGATGTACTATCTTGTCCTGTTGTAGTAGCTCCATTCATCATTCCACTTGTTCCATTAGCTAAAGCAGCAGGAATAACTCATTTTCCTGGAGTATTAAAATCTTCTCAACCTATCACTCTGGTTACATAA", "aa": "MLEPSNKPFLANSSKLPLRVRPSTSSFQLRKFVDSLLMYFESADVLSCPVVVAPFIIPLVPLAKAAGITHFPGVLKSSQPITLVT", "aa_hexdigest": "b5bfafef228a34c32e7120f9423cc423", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9225.878999999992, "isoelectric_point": 9.743316459655762 }, "id": "KMADJIBOEG_212", "locus": "GCA000292445_00173" }, { "type": "cds", "sequence": "contig_1", "start": 89596, "stop": 89802, "strand": "+", "frame": 1, "gene": null, "product": "PTS transporter subunit EIIC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF73E8" ], "nt": "ATGCTAATTGGAATTCCAGCAGCTGGACTTGCAATGATCTTAGCTGCTCCTAAGGGTAGAAGAAAAGAAGCTAGTTCTATTATTGGTAGTGCTGCATTCACTAGTTTTCTAACAGGGATCACCGAACCTTTTGAATTTACCTTTCTTTTCTTAGCACCATGGTTATTCTATGGTATCCACGCTGTATTAGCTGCAGTAAGCTTTTGA", "aa": "MLIGIPAAGLAMILAAPKGRRKEASSIIGSAAFTSFLTGITEPFEFTFLFLAPWLFYGIHAVLAAVSF", "aa_hexdigest": "c0e42e1ce3e2c51319e018cf78de9d4e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021AF73E8", "query_cov": 0.8088235294117647, "subject_cov": 0.9166666666666666, "identity": 0.5820000000000001, "score": 63.5, "evalue": 4.71e-12, "valid": false, "product": "PTS transporter subunit EIIC", "uniref50_id": "UniRef50_UPI0021AF73E8" }, "pscc": { "uniref50_id": "UniRef50_UPI0021AF73E8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF73E8" ], "product": "PTS transporter subunit EIIC" }, "genes": [], "id": "KMADJIBOEG_213", "locus": "GCA000292445_00174" }, { "type": "cds", "sequence": "contig_1", "start": 89806, "stop": 89898, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTATTGAGTGCTAACGTTGGACAAACCTTCTCAGGTTCTTTCATTGACTTTATCTTGTATGGGGCTTTACCTGATGGTAGGGGTTGA", "aa": "MNLLSANVGQTFSGSFIDFILYGALPDGRG", "aa_hexdigest": "3cf8f7a3c4b9e071c17bda66713ca988", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3161.5407000000005, "isoelectric_point": 4.207074546813964 }, "id": "KMADJIBOEG_214", "locus": "GCA000292445_00175" }, { "type": "cds", "sequence": "contig_1", "start": 89944, "stop": 90345, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00367.25" ], "nt": "TTGATTTATTTCCCTACCTTCTATTTCTTGACAATTCGCTTTAACTTAGCAACTCCTGGTAGAGGTGGTAAGTTAATTACTAAAAAAGAATATTTAGCAGCAAAAGCAGCTCAAAAAACTGATCAAACTACTATCACTAACTTTAATCAAACCCAAATTGAAGCTGGTATGTTACTAAGAGCTTATGGTGGAAGTGAAAACATTGCTGAATTAGGGGCTTGCATTACTAAATTAAGAGTAACAGTTAAAAACCCTGAACTTGTTAATGAAACTATTATTAAAGACTTGGGAGCAGCTGGGGTAATGCGTACCACTCCAACATTTTTTGTAGCAGTGTTTGATACTCGAGCTGCTGTTTATAAATCAGCAATGCAAGATATTATCCAAGGCAAAGTAAATTGA", "aa": "MIYFPTFYFLTIRFNLATPGRGGKLITKKEYLAAKAAQKTDQTTITNFNQTQIEAGMLLRAYGGSENIAELGACITKLRVTVKNPELVNETIIKDLGAAGVMRTTPTFFVAVFDTRAAVYKSAMQDIIQGKVN", "aa_hexdigest": "c8ac0444dd34f8baf31e99a8313dd96f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00367.25", "name": "PTS_EIIB", "length": 32, "aa_cov": 0.24060150375939848, "hmm_cov": 1.0, "evalue": 1.6973665701427051e-13, "score": 44.95527267456055, "start": 58, "stop": 89 } ], "seq_stats": { "molecular_weight": 14646.879699999983, "isoelectric_point": 9.587625312805173 }, "id": "KMADJIBOEG_215", "locus": "GCA000292445_00176" }, { "type": "cds", "sequence": "contig_1", "start": 90352, "stop": 90933, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00358.26" ], "nt": "TTGCAAAAAGTCTTAGATAAAAATGATAGTACTGTTGAAAAACCAGAAATAAAACCAACCCCAGTTTTAAAAGTTCAAGATGAAATTGTGATCCTCTCACCAGTTAATGGCACCTTAAAACCGCTCACCCAAGTTCCTGATGATACCTTCAAAAATCGTTTGGTAGGAGATGGAATTGCTATCTTACCTAGCGATGGGCACTTCAAAGCACCAGGTGATGTGGGTGTGAAAACTGAACTTGTTTTCCCTACTGGTCATGCCTTTATCTTTGATGTTGATGGTGTGAAAGTAATGCTTCACATTGGGATTGATACAGTAAAAATTAATGCTGATAAAAAACCAGGGGAACAACTTGAAGTGTTTGATGTTAAAACAAAACAAGGAGAATACACTAAATTAAAGAGTGAAAGTGTTGTTGAAGTTGATTTAAAGAAACTTAAACGAAAGTATGATCCAATCACTCCTTTCATTGTGATGCAAGAATCACTTGATAACTTCAAGTTGGTGCCAATTCGCCAACGTGGTGAAATTAAAGTTGGCCAACCTTTATTTAAACTAATTTATAAAGATAAGAAGAGTTAA", "aa": "MQKVLDKNDSTVEKPEIKPTPVLKVQDEIVILSPVNGTLKPLTQVPDDTFKNRLVGDGIAILPSDGHFKAPGDVGVKTELVFPTGHAFIFDVDGVKVMLHIGIDTVKINADKKPGEQLEVFDVKTKQGEYTKLKSESVVEVDLKKLKRKYDPITPFIVMQESLDNFKLVPIRQRGEIKVGQPLFKLIYKDKKS", "aa_hexdigest": "0c153a8e287148c7e8bf42c289c677c2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00358.26", "name": "PTS_EIIA_1", "length": 134, "aa_cov": 0.6839378238341969, "hmm_cov": 0.917910447761194, "evalue": 1.002797568535107e-29, "score": 98.00225067138672, "start": 30, "stop": 161 } ], "seq_stats": { "molecular_weight": 21589.960200000012, "isoelectric_point": 8.857456398010253 }, "id": "KMADJIBOEG_216", "locus": "GCA000292445_00177" }, { "type": "cds", "sequence": "contig_1", "start": 91044, "stop": 91136, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGATACAAATACTGAAAAACCTGAGTTAGTTTCCCTTAATAAGTTAAGTGAGATGCGCACTAACATCGGGATGGTTAAACGTTATTGA", "aa": "MSDTNTEKPELVSLNKLSEMRTNIGMVKRY", "aa_hexdigest": "6e9dafaa0e44b65bba63ebdcdfba1eed", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3486.004500000001, "isoelectric_point": 8.189886283874515 }, "id": "KMADJIBOEG_217", "locus": "GCA000292445_00178" }, { "type": "cds", "sequence": "contig_1", "start": 91146, "stop": 91361, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGATTCTTTATCGAACCTGAACGTAAGCATAATAACGATTTATTGAAGCTTGATCTACAGTACCAAGCGTTAAAAACTGCTTATAACTTCATTAAGGATGTTGTTAAAAATCACGGACAAATCCTTTTTGTTGGAACAAAGAATGATTATGTTAAAAAACTGGTAATTGATATTGCTAAAAGAGTTAATGTTGCATATATTACCCAGCGCTGA", "aa": "MGFFIEPERKHNNDLLKLDLQYQALKTAYNFIKDVVKNHGQILFVGTKNDYVKKLVIDIAKRVNVAYITQR", "aa_hexdigest": "0389f12c2bf01c4493ad346391329ca1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8338.6413, "isoelectric_point": 9.646162605285646 }, "id": "KMADJIBOEG_218", "locus": "GCA000292445_00179" }, { "type": "cds", "sequence": "contig_1", "start": 91620, "stop": 91898, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCACTATGTAACACCAACACCAATCCAGAGCTAGTTGACTTTATTATTCCAGCTAATAACCACCAACCCCAAAGTACTTGTTTATTGATGAATTTACTAGCAGATGCGATAGCAGAAGCGAAGGGCTTTGAAACCTTGTATGCTTACAAACCAGATGAACAGATCCAAATTGAAATTCCTCCCAAACAAGAACGCCAAGTTATTAACCGCTCCAATACCAGAAACATCACTAACCAGCGCTTAAACATTAACCGTCAGCAACAAGAAACTTTATAG", "aa": "MALCNTNTNPELVDFIIPANNHQPQSTCLLMNLLADAIAEAKGFETLYAYKPDEQIQIEIPPKQERQVINRSNTRNITNQRLNINRQQQETL", "aa_hexdigest": "c4ec16aa3b78c1dcdc7b7e018ba94fdf", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10563.82369999999, "isoelectric_point": 5.242680931091308 }, "id": "KMADJIBOEG_219", "locus": "GCA000292445_00180" }, { "type": "cds", "sequence": "contig_1", "start": 92236, "stop": 92631, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACAAAGTCTTTCACAAGGGTTTTTAGGAATGGTGAATTAATTAGCATTAATGTTAGTGAAGTTGTTGTAGGAGATATTATTTTTGTTGATGCAGGGGATATTATCCCTGCTGATGGCAAATTACTACAGGTTAATAACTTACGTTGTTTGGAAAGCTTTTTAACTGGTGAATCAACTCCAGTTGATAAGACTATTGATAGCAATGAAAAAGCTAGTATCCTTGAACAGACAAACTTAGTTTTTTCAGGGGCACAAGTAGTTTATGGTAGTGGCGTTTTTCAAGTGGAAGCAGTTGGGATTAAAACCCAAGTTGGACAAATTGCTAAAACTGTTGATGATAGTGTAACCAAGTTATCACCCTTACAACAAAAACTAGAGAAGATAGGAAAGTGA", "aa": "MTKSFTRVFRNGELISINVSEVVVGDIIFVDAGDIIPADGKLLQVNNLRCLESFLTGESTPVDKTIDSNEKASILEQTNLVFSGAQVVYGSGVFQVEAVGIKTQVGQIAKTVDDSVTKLSPLQQKLEKIGK", "aa_hexdigest": "edfe840a6bb84c4457154e58e530a404", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14099.967599999987, "isoelectric_point": 4.921483802795408 }, "id": "KMADJIBOEG_220", "locus": "GCA000292445_00181" }, { "type": "cds", "sequence": "contig_1", "start": 92791, "stop": 93063, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCTTTGCATTAAGTGTGCAGAAACTAACTAAGCAAAAAGCCATTATTAAGTATTTATCAGTAATTGAAACACTTGGATCAGTACAAATTATCTGTACTGATAAAACTGGTACTTTAACCCAAAACCAGATGAAAGTTGTCGATCACTTCTGTTTTAATTCAACAACCCAAACTGATCTAGCAAGAGCATTGTGTTTGTGTAATAATGCTTCTATTTCCAAAGATGCTAATAAAACAGGTGATCCTACTGAAATTGCTCTCTTGGAATGA", "aa": "MIFALSVQKLTKQKAIIKYLSVIETLGSVQIICTDKTGTLTQNQMKVVDHFCFNSTTQTDLARALCLCNNASISKDANKTGDPTEIALLE", "aa_hexdigest": "2e0900c010e0c7cab633e785977486aa", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9851.356999999993, "isoelectric_point": 7.646742820739747 }, "id": "KMADJIBOEG_221", "locus": "GCA000292445_00182" }, { "type": "cds", "sequence": "contig_1", "start": 93139, "stop": 93633, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00702.32" ], "nt": "ATGACAGTTGTTGTTCAAAAAGACAACCGCTTCATTGTGATTGTTAAAGGTGCTCCTGATGTGTTATTACCATTATGTAATAACGTTCAAAATGAAGTAAAGAACATTGAAAACTTACTTGATCAAAGTGCTGGTCAAGGCTTGCGTACCTTAGCAGTTGCTTTAAAGGTTTTATATAAGTTTGATCAAAACGATCAGAAGCAAATTGATGAACTTGAAAACAACCTTGAATTCCTTGGGTTTGTTAGTTTACAAGACCCACCAAGAAAAGAAAGTAAGGAAGCGATTTTAGCGTGCAAAAAAGCTAATATAACCCCAATAATGATTACAGGGGATCATCTTAAAACTGCAACTGCAATTGCTAAAGAGTTAGGCATTTTAACTTTAGATAATCAAGCAGTTTTAGGTAGTGAACTAGATGAAAAGAAGATCTTGGATTACAGGGTATTTGCTAGAGTAACTCCTCAACAAAAATTAGCCATTGTTAGTGCTTGA", "aa": "MTVVVQKDNRFIVIVKGAPDVLLPLCNNVQNEVKNIENLLDQSAGQGLRTLAVALKVLYKFDQNDQKQIDELENNLEFLGFVSLQDPPRKESKEAILACKKANITPIMITGDHLKTATAIAKELGILTLDNQAVLGSELDEKKILDYRVFARVTPQQKLAIVSA", "aa_hexdigest": "747f1e3afab5796d6d09481a84f7dbf2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00702.32", "name": "Hydrolase", "length": 122, "aa_cov": 0.7134146341463414, "hmm_cov": 0.8934426229508197, "evalue": 4.2622073995093303e-10, "score": 35.43714904785156, "start": 46, "stop": 162 } ], "seq_stats": { "molecular_weight": 18166.893999999986, "isoelectric_point": 5.883824729919433 }, "id": "KMADJIBOEG_222", "locus": "GCA000292445_00183" }, { "type": "cds", "sequence": "contig_1", "start": 93673, "stop": 94113, "strand": "+", "frame": 1, "gene": null, "product": "HAD-IC family P-type ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB434DA", "UniRef:UniRef90_UPI001FB434DA" ], "nt": "GTGAATGATGCACCTGCATTAATCAAGAGTGATGTAGGGTGTTGTATGGGGATTACTGGGGTTGATATTGCAAAAGATGCTAGTGATCTGATTATTAGTGATGATAATTTCGCTACTATAGTAAATGGTATTGAACAGGGTAGAAAAACCTTTTTAACTTGTAAACGAGTTTTATTAAACCTGTTTTTAACTTCAATTGCAGGAACAGTTGTAGTTTTATTAGGACTATTCATCTTAGGACAAGTTTTTAAAACTAATTTATTACAACAAGGTCATGACTTTCAGGTGTTTAGTCCTACCCAACTGCTAATTATTAACTTGTTTGTTCATGGTTTTCCTGCTGTTGCATTAGCAGTACAACCTGTTAAAGAAAAACTGATGGTAGGTAGTTTTTCTACTAAAAATTTGTTTTACAATCGCCAGGGCTTTGATTTAATCTGA", "aa": "MNDAPALIKSDVGCCMGITGVDIAKDASDLIISDDNFATIVNGIEQGRKTFLTCKRVLLNLFLTSIAGTVVVLLGLFILGQVFKTNLLQQGHDFQVFSPTQLLIINLFVHGFPAVALAVQPVKEKLMVGSFSTKNLFYNRQGFDLI", "aa_hexdigest": "1ac44b39b3fe3d0be902c730bf27ec19", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 93508, "stop": 94334, "observations": { "deletion": [ 94243 ], "mutation": [ 93631, 94111 ], "stop": [ 93631, 94111 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001FB434DA", "query_cov": 1.0, "subject_cov": 0.5270758122743683, "identity": 0.8290000000000001, "score": 241.0, "evalue": 2.93e-78, "gene_start": 1, "gene_end": 146, "reference_start": 56, "reference_end": 201, "reference_sequence": "MGILTDERQAILGVDLDPAKIMEYRVFARVTPQQKLEIVNAWKQAGYTVAVTGDGVNDAPALVTSDVGCCMGQTGVDIAKDAADVIISDDNFATIVNGIEQGRKTFLTCKRVLFNLFLTSIAGTIVVLLGLFVLGEVFREQLSKANHNFQVFTPTQLLIINLFVHGFPAVALAIQPVQEKLMLKPFSTKNLFYNRGGFDLIWQSLLLSFLTLLFYSLGMVYAINDPELGKSGDLINRAGATCGFMVLGGSAALNSLNLMVDRPLVATNPKHYGIVXX" }, "paralog": false, "identity": 0.7745454545454545, "score": 421.0, "evalue": 1.8e-151, "description": "Internal stop codon at 93631, 94111. Frameshift due to deletion around 94243. Nonsense mutation around 93631, 94111.", "psc": { "uniref90_id": "UniRef90_UPI001FB434DA", "product": "HAD-IC family P-type ATPase", "uniref50_id": "UniRef50_UPI001FB434DA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001FB434DA", "UniRef:UniRef50_UPI001FB434DA" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001FB434DA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB434DA" ], "product": "HAD-IC family P-type ATPase" } }, "id": "KMADJIBOEG_223", "locus": "GCA000292445_00184" }, { "type": "cds", "sequence": "contig_1", "start": 94368, "stop": 94526, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATCATCTTTATCAACCCTTTAGGGTTAGTGTTTAATGTCTTGCAAGATTTAACTAATCACCCAGTTTTAATAGGCTATAGTTTTGGGGGAGTTATTTTGTATATGGGGATGAATGAAGTTGTTAAACTTATTAGATTAGGTTATGGCAATATTTAA", "aa": "MIIFINPLGLVFNVLQDLTNHPVLIGYSFGGVILYMGMNEVVKLIRLGYGNI", "aa_hexdigest": "0b64592d09746ca2796fdda5cd1b239c", "start_type": "TTG", "rbs_motif": "TTCTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5780.9059, "isoelectric_point": 6.504506492614746 }, "id": "KMADJIBOEG_224", "locus": "GCA000292445_00185" }, { "type": "cds", "sequence": "contig_1", "start": 94513, "stop": 95388, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00270.35", "PFAM:PF07517.20", "PFAM:PF01043.26" ], "nt": "ATGGCAATATTTAACTTCCTTAAGTTAATTTCACCCAAAAACAGAATTCTCAGTAAGGCAAATAGGATTGCCAGTGAGGTTGAGAGTTATAAAAACTACTACCGTAACTTAACTGATCAACAGTTATTTGAAGAGTCAAATAAACTAGTTGATCTTGTCACTAAGCAAAATTACACCATTCTAGATGTTTGTGTTGCTGCACTTGCTTTAATTAGAGAAGTGGTTTACCGTGAGACTGGTGAATTTGCATATAGGGTGCAGATCATAGGAGCTTTTATTGTTTTAAGTGGTGATTTTGCTGAGATGATGACTGGTGAAGGTAAGACCTTAACCATTGTTTTAGCAGCATACGTTTCTGCACTTGAAAAGCGTGGTGTGCATGTTGTTACTGTCAATGAATATCTAGCTCAAAGGGATGCTAATAATGCAATGAAGATCTTAAAACGGGTTGGGATGAGTGTTGGTTGTAACTTTGCTAATCTCTCCCCTCAGCTAAAACAAGCTGCATTTAATTGCGATGTTACCTACACCACTAACAGTGAACTGGGGTTTGATTATCTTAGAGATAACATGGTCCACAGTTATCAAGATAAGAAGATCAGAGAGTTGCACTTTGCAATAGTTGATGAAGGTGATTCAGTTTTAATTGATGAGGCGCGAACGCCTTTAATTATTTCAGGTCCTAGTAAAAATGAGTTTGGGTTATATGTTGCAGTTGATCGATTTGTTAAATCATTAACTGAACAGGAGTTTAAGATTGACCCTGAATCACGTGCTGCTTCTTTAACTGAACTTGGGATTAAAAAAGCAGAGCAAACATTTAAAAAGAAAACCTTTTTGCTTTGGAAAACAGTGATCTTTTTCACAAGATCATGA", "aa": "MAIFNFLKLISPKNRILSKANRIASEVESYKNYYRNLTDQQLFEESNKLVDLVTKQNYTILDVCVAALALIREVVYRETGEFAYRVQIIGAFIVLSGDFAEMMTGEGKTLTIVLAAYVSALEKRGVHVVTVNEYLAQRDANNAMKILKRVGMSVGCNFANLSPQLKQAAFNCDVTYTTNSELGFDYLRDNMVHSYQDKKIRELHFAIVDEGDSVLIDEARTPLIISGPSKNEFGLYVAVDRFVKSLTEQEFKIDPESRAASLTELGIKKAEQTFKKKTFLLWKTVIFFTRS", "aa_hexdigest": "b48880d69ea9cc0f721183b99fa73c0a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00270.35", "name": "DEAD", "length": 121, "aa_cov": 0.4020618556701031, "hmm_cov": 0.9338842975206612, "evalue": 1.1853793082896228e-08, "score": 30.02716064453125, "start": 99, "stop": 215 }, { "id": "PF07517.20", "name": "SecA_DEAD", "length": 251, "aa_cov": 0.8625429553264605, "hmm_cov": 0.9601593625498008, "evalue": 1.4284206538789403e-90, "score": 298.5530700683594, "start": 11, "stop": 261 }, { "id": "PF01043.26", "name": "SecA_PP_bind", "length": 46, "aa_cov": 0.15463917525773196, "hmm_cov": 1.0, "evalue": 2.499074466444201e-07, "score": 26.458953857421875, "start": 234, "stop": 278 } ], "seq_stats": { "molecular_weight": 32994.62200000004, "isoelectric_point": 8.259834480285647 }, "id": "KMADJIBOEG_225", "locus": "GCA000292445_00186" }, { "type": "cds", "sequence": "contig_1", "start": 95355, "stop": 95759, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07517.20", "PFAM:PF01043.26" ], "nt": "TTGGAAAACAGTGATCTTTTTCACAAGATCATGAATGGTTTGACTGCTGTGAAAGTTTTTGAACAGGGCAAAGAGTACATTGTTCGTGATGGCAAGGTTTTAATTGTTGATCACTTTACAGGTAGGATATTGGAAGGGAGAAGTTACAGTAATGGCTTACAACAAGCTGTACAAGCCAAAGAATATGTTGAGATAGAACCTGAAAATGTGATAGTAGCTACCATTACCTACCAATCCTTCTTTAGGCTATACAACCGCTTAGCAGCAGTATCAGGTACTGCTTTAACTGAATCAGAGGAGTTTCTCAAGATTTATAACATGGTTGTAGTACCAGTGCCAACTAACCGTCCTAACATCAGAAAAGACCGTTCTGATAGTGTATTTGGTACCCCACAAATTAAGTGA", "aa": "MENSDLFHKIMNGLTAVKVFEQGKEYIVRDGKVLIVDHFTGRILEGRSYSNGLQQAVQAKEYVEIEPENVIVATITYQSFFRLYNRLAAVSGTALTESEEFLKIYNMVVVPVPTNRPNIRKDRSDSVFGTPQIK", "aa_hexdigest": "6a3c01ab69381d9036a3c243b5151f0a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07517.20", "name": "SecA_DEAD", "length": 53, "aa_cov": 0.39552238805970147, "hmm_cov": 1.0, "evalue": 3.9412038656307415e-16, "score": 54.23501205444336, "start": 49, "stop": 101 }, { "id": "PF01043.26", "name": "SecA_PP_bind", "length": 57, "aa_cov": 0.4253731343283582, "hmm_cov": 1.0, "evalue": 4.805716222609874e-22, "score": 73.8265609741211, "start": 2, "stop": 58 } ], "seq_stats": { "molecular_weight": 15229.229399999984, "isoelectric_point": 6.740558815002442 }, "id": "KMADJIBOEG_226", "locus": "GCA000292445_00187" }, { "type": "cds", "sequence": "contig_1", "start": 95760, "stop": 96674, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF21090.3" ], "nt": "ATGGCAGTTGTTAAAGAGATAAAAAAGATCCATGAAACTTCTCGACCTATTCTGATTGGAACTGCTAACATAGATGATTCTGAACTCTTACATAATCTGTTACTAGAAGCTAATATTCCCCATGAGGTTTTAAATGCTAAAAACCATTCAAGAGAAGCGGAGATAGTAGCTAAAGCAGGACAGAAGAATGCAGTGACTATTTCAACTAACATGGCTGGAAGAGGAACTGATATCCGTTTAGGTGAAGGGGTTGCTGAAATGGGTGGTCTTTATGTATTGGGAACTGAAAGAAATGAGTCAAGAAGGATTGATAACCAACTAAGAGGGAGAGCTGCTAGACAAGGTGATAAAGGGGAAACTAAGTTCTTTATCTCACTAGGTGATTCATTGTTTAAACGTTTTGCTCATGACAAGATTGAAAGAGCGATTAGCAAATTAGGTAATGAAACATTTGACAGTGCCTTCTTTTCCAAAATGTTAAGTAGAACCCAAAAACGGGTGGAAGCAATTAACTTTGACACTAGAAAAAACCTGATTGATTATGACCATGTTCTTGCAAGTCAAAGGGAATTGATTTACAAACAACGTGATAAGTTTTTATTAGCAAACGATTTAAGTGAAATGATCGACAAAATGCTAGAAAAGTTTGTACAACAGTTTTGTGATCAATATAGAAACCAAAAGAACCAAAACTTAATTAATCACATTGCACTAGCAGAAGCTTTAAATCTTGAGATGAACATGCAAAACACCATTAATCCAAAGGTGTTTGAAAACATGACTTTTGATGTTGCTGTTGATAAAACCCGTAACTTAGTAGCTAAAAAGATTAGTGATAAAGTTAATGTTTTGACCAAACCAATTGCTTTAAACAGGTTTCGTGACATTATCATAACTTCGATGGATAAACATTGA", "aa": "MAVVKEIKKIHETSRPILIGTANIDDSELLHNLLLEANIPHEVLNAKNHSREAEIVAKAGQKNAVTISTNMAGRGTDIRLGEGVAEMGGLYVLGTERNESRRIDNQLRGRAARQGDKGETKFFISLGDSLFKRFAHDKIERAISKLGNETFDSAFFSKMLSRTQKRVEAINFDTRKNLIDYDHVLASQRELIYKQRDKFLLANDLSEMIDKMLEKFVQQFCDQYRNQKNQNLINHIALAEALNLEMNMQNTINPKVFENMTFDVAVDKTRNLVAKKISDKVNVLTKPIALNRFRDIIITSMDKH", "aa_hexdigest": "ad9c5f218455bf6bddd4cef68ae756cd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF21090.3", "name": "P-loop_SecA", "length": 81, "aa_cov": 0.26644736842105265, "hmm_cov": 1.0, "evalue": 9.989885597740352e-64, "score": 209.8253936767578, "start": 2, "stop": 82 } ], "seq_stats": { "molecular_weight": 34671.368500000026, "isoelectric_point": 9.228987693786618 }, "id": "KMADJIBOEG_227", "locus": "GCA000292445_00188" }, { "type": "cds", "sequence": "contig_1", "start": 96684, "stop": 96932, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATAGTGTTTTTAAGTTAAGAGAAGGGGTTGTACTTCGTTCTATGGAACATACAAGTCCTTTAAATGTTTACATTAAAGAAACAGATATCCTTTTTAAAACAATGTTGCAAAAGATTGCTCAAGATGTCATTGTGCAAATTGCTAACCTCACAACTCCAGATGAATTTGATCATAGCTTAATGCAAGCCAATGCTTTAAAGAAACTAGCAGCAATTAAAGCAGATGAAAAATCAAACCAAGAGTAA", "aa": "MDSVFKLREGVVLRSMEHTSPLNVYIKETDILFKTMLQKIAQDVIVQIANLTTPDEFDHSLMQANALKKLAAIKADEKSNQE", "aa_hexdigest": "cb6e634dd1f2b81062d3800bf3643a62", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9303.670099999994, "isoelectric_point": 5.623217964172363 }, "id": "KMADJIBOEG_228", "locus": "GCA000292445_00189" }, { "type": "cds", "sequence": "contig_1", "start": 96910, "stop": 98880, "strand": "+", "frame": 1, "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "db_xrefs": [ "COG:COG0556", "COG:L", "GO:0003677", "GO:0005524", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432", "GO:0016887", "KEGG:K03702", "RefSeq:WP_014894137.1", "SO:0001217", "UniParc:UPI00027B376E", "UniRef:UniRef100_UPI00027B376E", "UniRef:UniRef50_P47319", "UniRef:UniRef90_P47319" ], "nt": "ATGAAAAATCAAACCAAGAGTAATAGTTTATTTCAACTTTCCACTAACTATATACCTACTGGTGATCAACCTGAAGCAATTAAGAAATTATCAGAATTTAAAACTAAGCAGCAGGTTTTATTGGGGGCCACAGGCACAGGTAAAACCTTTACAATTGCTAATGTAATTCAAAACAGCCAACTCCCAACAGTTGTTATTGCTCATAACAAAACCCTAGCAGGTCAACTCTTCAATGAATTAAAGCAACTGTTTCCTAAAAATGCAGTTGAATATTTTATCTCTTACTTTGATTTTTATCAACCTGAAGCTTACTTACCCAGTAAAGGGATCTACATTGAAAAAAGTGCTACAGTCAATGAAGCGATTAAACGCTTAAGAGTCTCAACACTGCATTCACTTTCAACAAGAAAAGATGTTATTGTAGTAGGTTCTGTTGCTAGTATTTATCCCACCTCATCTCCCAGTGATTTTGTTAAGTATTGCTTGTGGTTTGTGGTTGGCAAAGATTATGATTTGAAAACCATTAAAGATAGGTTAGTTAGTCTTAACTATGTTGTTAATAAACAACAATTAACCCCAGGAAAATTTCGCTTTCAGGGTGATGTTTTGGAGGTATTTCCTGGTTACAGTGATGCTTTTGTGATCAGAATCTCCTTTTTTGATACTAAAGTAGAACAAATTTGTCAAATTGACCCACTAACAAATAAGATTTTAAACCAACTCTTTGAGATTAAGATAGGTCCTGCTGATGAATATGTTGTAAACCAATCTGATCTTGATATAGCAATTAAAAATATTAAACAAGAACTTCAGGAACGAGTTAATTATTTCAATAAGCAAAATCTTGTTGAAAGAGCACAACGTTTAGCCACCATTACTAACCATGATCTCAATGATCTGAAGGCTTGGGGATTTTGTAGTGGAGTTGAAAACTATGCTAGACACTTAGAGTTGAGGATGGCTAACTCAACCCCTTACAGTATCTTTGATTATTTTAAGGGGGATTGGTTATTGGTTATTGATGAATCACACCAAACTTTACCGCAACTTAATGGGATGTATAACACTGATCTTTCAAGAAAACAAAGCTTAATTGATTATGGTTTTCGACTCCCCTCTGCACTTGATAACAGACCGCTCTCATTTGCTGAATTACAACAAAAAATGCAAAAAGTTATTTATGTTTCAGCAACTCCAAGAGATAAAGAGATTAGTTTAAGTCAGAATAATGTCATTGAACAGTTAGTTAGACCAACCTACTTGGTTGATCCTATTATCGTTGTTAAACCAAAAGATAACCAGGTGGAGGATCTCATTGAAGAGATTATCAACCAACGCCAAAACAACACAAGAACATTTGTTACTGTTTTAACTATTAAGATGGCTGAAAACCTCACTGAATACTTAAAGGAACGCAAAATTAAAGTTGCCTATATCCATAAGGACATTAAAGCATTGGAACGTTTATTGTTAATTAATGACCTGAGAAGAGGTGAATATGAGTGTTTAGTTGGGATTAACCTTTTAAGAGAAGGGTTAGATGTCCCTGAAGTTGCTTTAGTTTGTATCTTTGATGCAGATATCCCAGGACTACCTAGGGATGAGAGAAGTTTAATCCAGATTATTGGACGTGCTGCTAGAAATGAACATGGTCGAGTTGTTATGTATGCTAACCATGTTACTGAACAGATGCAAAAAGCCATTGATGAAACCAAAAGAAGAAGAACTGTTCAAATGGAATATAACAAGCTACATAATAAGATACCAAAAACAGTTGTTAAACCCCTTAGCTTTGTTCAACCAATCAAATTAAAAGCTAAGAGTAATGCAGAAAAAAATGCTGCATTAATCAAACAATTAACCAAAGAAATGAAGAAAGCAGCAGCTAATCAAAATTATGAACTTGCCATTGAGATTAGAGATTCCATATTTGAATTGGAAAAAGAAATTGATAGTAAAATTAAAGTATAG", "aa": "MKNQTKSNSLFQLSTNYIPTGDQPEAIKKLSEFKTKQQVLLGATGTGKTFTIANVIQNSQLPTVVIAHNKTLAGQLFNELKQLFPKNAVEYFISYFDFYQPEAYLPSKGIYIEKSATVNEAIKRLRVSTLHSLSTRKDVIVVGSVASIYPTSSPSDFVKYCLWFVVGKDYDLKTIKDRLVSLNYVVNKQQLTPGKFRFQGDVLEVFPGYSDAFVIRISFFDTKVEQICQIDPLTNKILNQLFEIKIGPADEYVVNQSDLDIAIKNIKQELQERVNYFNKQNLVERAQRLATITNHDLNDLKAWGFCSGVENYARHLELRMANSTPYSIFDYFKGDWLLVIDESHQTLPQLNGMYNTDLSRKQSLIDYGFRLPSALDNRPLSFAELQQKMQKVIYVSATPRDKEISLSQNNVIEQLVRPTYLVDPIIVVKPKDNQVEDLIEEIINQRQNNTRTFVTVLTIKMAENLTEYLKERKIKVAYIHKDIKALERLLLINDLRRGEYECLVGINLLREGLDVPEVALVCIFDADIPGLPRDERSLIQIIGRAARNEHGRVVMYANHVTEQMQKAIDETKRRRTVQMEYNKLHNKIPKTVVKPLSFVQPIKLKAKSNAEKNAALIKQLTKEMKKAAANQNYELAIEIRDSIFELEKEIDSKIKV", "aa_hexdigest": "f38e7774a97159727097d59dac6d8ced", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B376E", "ncbi_nrp_id": "WP_014894137.1", "uniref100_id": "UniRef100_UPI00027B376E", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B376E", "RefSeq:WP_014894137.1", "UniRef:UniRef100_UPI00027B376E" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B376E", "uniref90_id": "UniRef90_P47319" }, "psc": { "uniref90_id": "UniRef90_P47319", "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "uniref50_id": "UniRef50_P47319", "cog_id": "COG0556", "cog_category": "L", "kegg_orthology_id": "K03702", "go_ids": [ "GO:0003677", "GO:0005524", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47319", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47319" ], "product": "UvrABC system protein B" }, "genes": [ "uvrB" ], "id": "KMADJIBOEG_229", "locus": "GCA000292445_00190" }, { "type": "cds", "sequence": "contig_1", "start": 98883, "stop": 99089, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MG074", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47320", "UniRef:UniRef90_P47320" ], "nt": "ATGCGACAGTTTATTAAGCTTAGCCTCTTAGTCTTTGTCTTGCTTTTCTTAAGTGAACTTATCTGTAGGTTCTCTTTACGCTTAGTAAATTCTATTAAAGCGAGATATAAATTTTCAGTATTTTCCTACACTGCTTGTTTGCTATTTTTAAAGAGTTTTCAAAACTTTTCTAATGCTTTTCAGAAGTTAGCTAACTGGGTATTTTGA", "aa": "MRQFIKLSLLVFVLLFLSELICRFSLRLVNSIKARYKFSVFSYTACLLFLKSFQNFSNAFQKLANWVF", "aa_hexdigest": "6851474f67679feb0a5f7f271d438581", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 98883, "stop": 99296, "observations": { "mutation": [ 99087, 99273 ], "stop": [ 99087, 99273 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47320", "query_cov": 1.0, "subject_cov": 0.49635036496350365, "identity": 0.985, "score": 122.0, "evalue": 2e-34, "gene_start": 1, "gene_end": 68, "reference_start": 1, "reference_end": 68, "reference_sequence": "MRQFIKLSLLVFVLLFLSELICRFSLRLVNSIKARYKSSVFSYTACLLFLKSFQNFSNAFQKLANWVFWFENDVNELLSIFYFNFDQKSEKVDYNFFNGYKVTAQKVVEKEQLLTCKLSDYYRLFRDKTFWFELINN" }, "paralog": false, "identity": 0.9781021897810219, "score": 265.0, "evalue": 2.81e-93, "description": "Internal stop codon at 99087, 99273. Nonsense mutation around 99087, 99273.", "psc": { "uniref90_id": "UniRef90_P47320", "product": "Uncharacterized protein MG074", "uniref50_id": "UniRef50_P47320", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47320", "UniRef:UniRef50_P47320" ] }, "pscc": { "uniref50_id": "UniRef50_P47320", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47320" ], "product": "Uncharacterized protein MG074" } }, "id": "KMADJIBOEG_230", "locus": "GCA000292445_00191" }, { "type": "cds", "sequence": "contig_1", "start": 99360, "stop": 101756, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MG075", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47321", "UniRef:UniRef90_P47321" ], "nt": "ATGAAATTAAGTACAATAACTACTATCTGCTTGTCAATATCTGGTGCTTTTGGTACTACTGCAATTGCTTTACCAACTACAGTAGCATTGTTAAAAAACCACCAACAGCAAAACACTGAAAAACAACAAAACCCGATTAAAGATATCCGCTTTGGTTTAAACAATGTACAGGTTCCAAATACCATTCCGTTACACCAAACTGTGGTTGAAGTGATCAACAATAAAGAAATTGTTGATTACAAAGATGCACCCCAAAAACTCTTTTTAGCTAAAAGTGCGTTAAATAATAAACTCCAAGTTGAGTTTGATAAGTTCTTGCTAAGAACAGGAGTAATTAATGCTTTAAATGCAGATTTGAAAGAATGGATAGACCAAACATTATTTATCCCCAATCAAAGTTTTTTTGATCTTAGTGCTAATAAACTCAATTTAACTTTGTCAAATCAAGGCGAAGTGAGTTTAGATCTTGATTTTACTTTTACTAACTTTAGTGATAAAAATCAACCATTAAAACTCCCCTTTGACGGTAATGTGGTGGTTAATGCTAACGAATCATACACCTATTCAGTTAAAGCAACACTACAGAAACTAAAAGTATTGACTTTTTCAAGAGCAGATCATTATGTAGGGATTAGTTATGCAATACCAAAAGTGAGTTTAAATGGTAAAACTCAAAATGACTTTAGTTTTAACCCTTTTAAAAGTAATATTAACTTTACTTTTAAAAATGTCTACAATGCTTTAGATCCGTTTGAAGCACAAAAATACCTGGTTGGCCAAGGCAAGTTTTTAAACCAAAAAGTTAATGCTGATGATGTTAAAAATGACATTAATAACCACATTGAAACCCAGTTTAATGTTGCTAAAATCACAGCAACTCTTTTAGGAAAAGCTTTTAAGCAGTTTGGTGAACATAAAAATGGTCAACCACTTTCACTTTTAAAAGTACTAAGTGGATTAAACAATGAGTTTAAACAACTCTTTAATTATGTCAGACCTGGTTTAGGTGATTTTGTTAGTGATTTAATCCAAAGCTCCAGTCAATCAAGTAATAAAAAAACTGTTTACCAGCTATTATTTGAAAACAAGACAACAATTATCCACTTGTTGCAAGATCTTAATATTAGTGAACTGAACTCTGTTTTACCAGTTGTTGATATTTTGTTTGAAGGGATTAACAGTGCAGAATCACTATACCAAAGGATCCAATCATTTAAGGATTTAATTGTTCCTGCTTTAAAAGCTGATAAGCAACTTAAGAGCTTAGAAGCGATTATTTTAGCAGTTTTAGACAATCCTAATACCTATGTTTTTGATCTTGTTTACCAAAACAAATCGATTCTGTTTAATTTGTTAAGTGATTTTCTTAAAAACACCGCAAACACTTTACCATTTCTTCAAGAACAGTTTGATATTGTTAACCACTTATTTGCCAATGAAGCGATCTTTGATCTGTTTTCCAATGCTGACTTTGTTGATAAGATTGCTGATCTTTTTTTAGCAAAACAAAAGGTACAAGAAGTTAATAATGATGGAACTAAATCAACAAAAATTGTTGATTCGATTTTAGTTGCTACCCTTAAGGGTTTAGTTGGTAATCAGCTCAGTTCAATCACTGAACTGTTAAACATCTATATCTTTGAAAATGAATTTTTAAACAGAAATGATAGTAATAGTTCAGTTAAAAAACAACAAACTGATAGTCTAAAAAATCTGTTTAGCGTAATTGGTGATATTTTAAGTGAGACCAATGTTAACAAGATCACTTTACACGCAGTTAAAAATAATGAACTTCTTTCGTTAGTTGAAACAGCTTCAACACTAAAAATTAAACATCTTAATGTTCAATACAAAGTTTTAGTTGATAAGTTTGAATTGAAAAACAGTTTCATTAAGGCACTATTAAATTTTTTCCCAGATACTAAAGATATCACCCCAACTATTAAAAAGGTACTGTTTGAAAGTGAAAATTATAAAACACTCCGTAAGAAGTATGAAAATGAAGGGTTCCCAGGTTATCACTGGGCTAAGTTCATTGTCCCAGGTACCTTCAATTCTGCTGAAAACACTTTCTATTCTGCTATTGATAAAACTAAATCAATTCGCGATCTGTTTGCTGACATGTTATTTGGTAAGAGTTTAGAGAGTGTTAATGACAGTGATAGTTTTATCAAAATTAATGGTTCATTTACATTAAAGTACCACGGTGATAACTTAAATTTACTCCCTAACTACCACTCATTAATCACTAAGAATGTTGGTTATCAAATAGTTAATGTTAATTTTCATATTGATGCAAGATTGCTAACTGCAGAACTACAAAACACGGTATTTTCAAATCCAAAACCAGTTATTAAATCACCTGTTGAACTCTCCAAATCCTTATTTGAAGTTTGA", "aa": "MKLSTITTICLSISGAFGTTAIALPTTVALLKNHQQQNTEKQQNPIKDIRFGLNNVQVPNTIPLHQTVVEVINNKEIVDYKDAPQKLFLAKSALNNKLQVEFDKFLLRTGVINALNADLKEWIDQTLFIPNQSFFDLSANKLNLTLSNQGEVSLDLDFTFTNFSDKNQPLKLPFDGNVVVNANESYTYSVKATLQKLKVLTFSRADHYVGISYAIPKVSLNGKTQNDFSFNPFKSNINFTFKNVYNALDPFEAQKYLVGQGKFLNQKVNADDVKNDINNHIETQFNVAKITATLLGKAFKQFGEHKNGQPLSLLKVLSGLNNEFKQLFNYVRPGLGDFVSDLIQSSSQSSNKKTVYQLLFENKTTIIHLLQDLNISELNSVLPVVDILFEGINSAESLYQRIQSFKDLIVPALKADKQLKSLEAIILAVLDNPNTYVFDLVYQNKSILFNLLSDFLKNTANTLPFLQEQFDIVNHLFANEAIFDLFSNADFVDKIADLFLAKQKVQEVNNDGTKSTKIVDSILVATLKGLVGNQLSSITELLNIYIFENEFLNRNDSNSSVKKQQTDSLKNLFSVIGDILSETNVNKITLHAVKNNELLSLVETASTLKIKHLNVQYKVLVDKFELKNSFIKALLNFFPDTKDITPTIKKVLFESENYKTLRKKYENEGFPGYHWAKFIVPGTFNSAENTFYSAIDKTKSIRDLFADMLFGKSLESVNDSDSFIKINGSFTLKYHGDNLNLLPNYHSLITKNVGYQIVNVNFHIDARLLTAELQNTVFSNPKPVIKSPVELSKSLFEV", "aa_hexdigest": "7b81220e887c898cff52189fc4fbdab8", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 99360, "stop": 102058, "observations": { "deletion": [ 101964 ], "mutation": [ 101754 ], "stop": [ 101754 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47321", "query_cov": 1.0, "subject_cov": 0.779296875, "identity": 0.98, "score": 1511.0, "evalue": 0.0, "gene_start": 1, "gene_end": 798, "reference_start": 1, "reference_end": 798, "reference_sequence": "MKLSTITTICLSISGAFGTTAIALPTTVALLKNHQQQNTEKQQNPIKDIRFGLNNVQVPNTIPLHQTVVEVTNNKAIVDYKDAPQKFFLAKSALNNKLQVEFDKFLLRTGVINALNADLKEWIDQTLFIPNQSFFDLSANKLNLTLSNQSEVSLDLEFIFTNFSDKNQPLKLPFDGSVVVNANESYTYSVKATLQKLKVLTYSRADHSVGISYAIPTVSLNGKTQNDFSFNPFKSNINFAFKNVYNALNPFEAQQYLVGQGKFLNQKVNADDVKNDINNHIETQFNVAKITATLLGKAFKQFGEHKNGQPLSLLKVLSGLNNEFKQLFNYVRPGLGDFVSDLIQSSSQSSNKKTVYQLLFENKTTIIHLLQDLNISELNSVLPVVDILFEGINSAESLYQRIQSFKDLIVPALKADKQLKSLEAIILAVLDNPNTYVFDLVYQNKSILFNLLSDFLKNTANTLPFLQEQFDIVNHLFANEAIFDLFSNADFVEKIADLFLAKQKVQEVNNDGTKSTKIVDSILVATLKGLVGDQLSSITELLNIYIFENEFLNRNDSNSSVKKQQTDSLKNLFSVIGDILSETNVNKITLHAVKNNELLSLVETASTLKIKHLNVQYKVLVDKFELKNSFIKELLNFFPDTKDITPTIKKVLFESENYKTLRKKYENEGFPGYHWAKFIVPGTFNSAENTFYSAIDKTKSIRDLFADMLFGKSLESVNDSDSFIKINGSFTLKYHGDNLNLLPNYHSLITKNVGYQIVNVNFHIDARLLTAELQNTVFSNPKPVIKSPVELSKSLFEVWKTIFENSVNQILKKEYTFKDNLKFFPFKADGSSRLEFDLSKPDQRVIPFAFVDGYQFQLKKELIPNKETKKEANSSPVLKLYDAVKRNDRQYRPNHHHDDLRNYPSLKSQLELILNLGDKLKANNDFIDDTVVNALQYKTSFKSTLKVNSLGIPINLFFFTLWLKFNLEIPIDGSLTLTSVNVVFPYSLYDTSSNEFTRIVDRLNFTDTNFYLKDAFPNFWFVGF" }, "paralog": false, "identity": 0.9788888888888889, "score": 1716.0, "evalue": 0.0, "description": "Internal stop codon at 101754. Frameshift due to deletion around 101964. Nonsense mutation around 101754.", "psc": { "uniref90_id": "UniRef90_P47321", "product": "Uncharacterized protein MG075", "uniref50_id": "UniRef50_P47321", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47321", "UniRef:UniRef50_P47321", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47321", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47321" ], "product": "Uncharacterized protein MG075" } }, "id": "KMADJIBOEG_231", "locus": "GCA000292445_00192" }, { "type": "cds", "sequence": "contig_1", "start": 102079, "stop": 102372, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED933E1", "UniRef:UniRef90_UPI001ED933E1" ], "nt": "GTGAAATTTAAGCGATCGACTATCCTTGTAAATTCATTTGAACTAGTATCATACAAACTGTATGGGAAAACAACATTAACAGATGTTAATGTTAATGAACCATCAATTGGAATTTCTAAGTTAAATTTAAGTCACAATGTGAAAAAGAAAAGGTTAATAGGAATTCCTAAACTGTTAACTTTTAGAGTAGATTTAAAACTAGTTTTATATTGCAAAGCATTGACAACAGTGTCATCTATAAAGTCATTATTAGCTTTAAGCTTATCACCAAGGTTTAATATAAGTTCTAGTTGA", "aa": "MKFKRSTILVNSFELVSYKLYGKTTLTDVNVNEPSIGISKLNLSHNVKKKRLIGIPKLLTFRVDLKLVLYCKALTTVSSIKSLLALSLSPRFNISSS", "aa_hexdigest": "f30abffff84eb9517c543fefceed5ee2", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001ED933E1", "query_cov": 1.0, "subject_cov": 0.941747572815534, "identity": 1.0, "score": 180.0, "evalue": 3.25e-57, "valid": true, "uniref50_id": "UniRef50_UPI001ED933E1" }, "pscc": { "uniref50_id": "UniRef50_UPI001ED933E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED933E1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10843.832299999985, "isoelectric_point": 10.297228813171383 }, "id": "KMADJIBOEG_232", "locus": "GCA000292445_00193" }, { "type": "cds", "sequence": "contig_1", "start": 102427, "stop": 102843, "strand": "-", "frame": 1, "gene": null, "product": "MPN214 family protein", "db_xrefs": [ "GO:0005886", "RefSeq:WP_014894139.1", "SO:0001217", "UniParc:UPI00027B3770", "UniRef:UniRef100_UPI00027B3770", "UniRef:UniRef50_P47322", "UniRef:UniRef90_P47322" ], "nt": "ATGGTTTTAAATCAAAACAAAAACAGTAATAAAGCTGAGTATGGTGGGATAGTTATTAGCGTATTTTATCTAATCCTTTTCTTTTTGATCTTAAACATTACTATTTATTTTCACAAGTCAACCAATTTTACAGTGGTTGTTAAAAATTCAGTACTAACTAGCTTTTTTGTTAACTTATTACTTGTTTGTTTACAGGGGATATTCCGTTTAAAAACTTGTGATGGAATGCGGTATGAAATTAGTAAGTTTAACAGATATCTTAAGTTAGGTAGTGTTTATGCAAAACCATTAGTTAGTTTTAATCAATATCAAGATCAAAGTGCAACTTACAGACAAAAAACTAGTGGGTTTTGGTGGATGAACTTGATTGTTTATCTAGTTGGTAGTTTAGTTAGTGGTTTAGTTTCATTACTCTAG", "aa": "MVLNQNKNSNKAEYGGIVISVFYLILFFLILNITIYFHKSTNFTVVVKNSVLTSFFVNLLLVCLQGIFRLKTCDGMRYEISKFNRYLKLGSVYAKPLVSFNQYQDQSATYRQKTSGFWWMNLIVYLVGSLVSGLVSLL", "aa_hexdigest": "0827c1c51961f34a09cfb8393eaf5802", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3770", "ncbi_nrp_id": "WP_014894139.1", "uniref100_id": "UniRef100_UPI00027B3770", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3770", "RefSeq:WP_014894139.1", "UniRef:UniRef100_UPI00027B3770" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3770", "uniref90_id": "UniRef90_P47322" }, "psc": { "uniref90_id": "UniRef90_P47322", "product": "MPN214 family protein", "uniref50_id": "UniRef50_P47322", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47322", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47322" ], "product": "Uncharacterized protein MG076" }, "genes": [], "id": "KMADJIBOEG_233", "locus": "GCA000292445_00194" }, { "type": "cds", "sequence": "contig_1", "start": 103075, "stop": 103275, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCAAGTATATTCTTAAACGATTAGGACTAGCAGTAGTTGCGATGTTTATCGTAATGTCTATAGTCTTCTTTTTAGTGAACGCTACTGGTAATGTTCCCTTGTCAGCCACTTCTGCAAGAGATATTGCTGCAGTGCAAGCACAACTACAAGAGTTTGGGTTTAATGACCCTATTATAATTAGGTATTTTCGCTATTGA", "aa": "MFKYILKRLGLAVVAMFIVMSIVFFLVNATGNVPLSATSARDIAAVQAQLQEFGFNDPIIIRYFRY", "aa_hexdigest": "6c4c6fd0e8e51a77175d485ed3d3876c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7460.824100000001, "isoelectric_point": 9.815069770812986 }, "id": "KMADJIBOEG_234", "locus": "GCA000292445_00195" }, { "type": "cds", "sequence": "contig_1", "start": 103351, "stop": 104094, "strand": "+", "frame": 1, "gene": "oppB", "product": "Oligopeptide transport system permease protein OppB", "db_xrefs": [ "COG:COG0601", "COG:EP", "GO:0005886", "GO:0015031", "GO:0015833", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P75554", "UniRef:UniRef90_P47323" ], "nt": "GTGTTTGCAAGAGTACCAAATACCTTATATGTGGTTTTAATCTCTTTTTTAATTGGTTCATTGCTAGGGATCTTTTTAGGGATGGTTTCAGGATTGAATAGAGGGAAGTTTTTAGATGCAGCAATTAATGTGTTGGTAGTTTTATTTGTATCTATTCCTTCATTTGTAGTGGGATTAGGGTTACTTAAACTAGCAGGATTTTTAAATCTACCACCACGGTTTATTAACTTTGATGATGCTTTTTTTAGCTTTGATCGTTTCTTGCTTGCATCAATTATCCCGATCCTTTCATTGGTCTTCTATTCATCAGCTGCTTTTACATACAGGATTAGAAATGAGGTGGTGGAAGTGATGAATCAAGACTATATTAAAACTGCAAAAAGTAAGGGACTTGGGATGTTTGCTGTAGCTAGGTATCATATCTTTAGAAACTCGATTATTCCTTCTATTCCCTTGTTTGTATTTGGAATCTCAGGTGCTTTTTCAGGTGGATTTATTATTGAGTCTTTGTTTGGAGTACAAGGGGTATCTAGGATCTTAATTGATTCAGTGCAAGTTAATGAAACTAACATGGTAATGTTTAATATCTTGTTTATCCAAGGGATTCCCTTATTAGCAAGTGTCTTTATTGAATTTATCTATGTTTTAGTTGATCCTAGAATTAGGATTGCAAATAGTTCTAATGTTAGCTTATTAACTAAGTTAAAGTTCTTAAGTTCAAGACACCAATGGTTAATGAAGTGA", "aa": "MFARVPNTLYVVLISFLIGSLLGIFLGMVSGLNRGKFLDAAINVLVVLFVSIPSFVVGLGLLKLAGFLNLPPRFINFDDAFFSFDRFLLASIIPILSLVFYSSAAFTYRIRNEVVEVMNQDYIKTAKSKGLGMFAVARYHIFRNSIIPSIPLFVFGISGAFSGGFIIESLFGVQGVSRILIDSVQVNETNMVMFNILFIQGIPLLASVFIEFIYVLVDPRIRIANSSNVSLLTKLKFLSSRHQWLMK", "aa_hexdigest": "b7c521ba66acfb3ac96f6e00bcf2a0b3", "start_type": "GTG", "rbs_motif": null, "genes": [ "oppB" ], "truncated": "both", "pseudogene": { "start": 103075, "stop": 104298, "observations": { "mutation": [ 103273, 104092 ], "stop": [ 103273, 104092 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47323", "query_cov": 0.9959514170040485, "subject_cov": 0.6044226044226044, "identity": 1.0, "score": 440.0, "evalue": 5.17e-153, "gene_start": 2, "gene_end": 247, "reference_start": 94, "reference_end": 339, "reference_sequence": "MFKYILKRLGLAVVAMFIVMSIVFFLVNATGNVPLSATSARDIAAVQAQLQEFGFNDPIIVRYFRYWAKLFSFQADALGIYYANPNQTIGEIVFARVPNTLYVVLISFLIGSLLGIFLGMVSGLNRGKFLDAAINVLVVLFVSIPSFVVGLGLLKLAGFLNLPPRFINFDDAFFSFDRFLLASIIPILSLVFYSSAAFTYRIRNEVVEVMNQDYIKTAKSKGLGMFAVARYHIFRNSIIPSIPLFVFGISGAFSGGFIIESLFGVQGVSRILIDSVQVNETNMVMFNILFIQGIPLLASVFIEFIYVLVDPRIRIANSSNVSLLTKLKFLSSRHQWLMKWNKINSDNAQNIVFNSPLHHQLLELNAIDYKTKTVQLTTEQKTALNISATANFILLGNKCLKLKTIHG" }, "paralog": false, "identity": 0.9926289926289926, "score": 761.0, "evalue": 4.1e-282, "description": "Internal stop codon at 103273, 104092. Nonsense mutation around 103273, 104092.", "psc": { "uniref90_id": "UniRef90_P47323", "gene": "oppB", "product": "Oligopeptide transport system permease protein OppB", "uniref50_id": "UniRef50_P75554", "cog_id": "COG0601", "cog_category": "EP", "go_ids": [ "GO:0005886", "GO:0015833", "GO:0015031", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47323", "UniRef:UniRef50_P75554", "COG:COG0601", "COG:EP", "GO:0005886", "GO:0015833", "GO:0015031", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P75554", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75554" ], "product": "Oligopeptide transport system permease protein OppB" } }, "id": "KMADJIBOEG_235", "locus": "GCA000292445_00196" }, { "type": "cds", "sequence": "contig_1", "start": 104128, "stop": 104298, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTAACTCGCCACTGCACCACCAGTTATTAGAACTCAATGCAATTGATTACAAAACAAAAACAGTCCAACTAACAACTGAACAAAAAACTGCTCTCAATATCAGTGCAACTGCTAACTTTATCTTACTTGGTAACAAGTGTTTAAAACTCAAAACAATCCATGGATAG", "aa": "MFNSPLHHQLLELNAIDYKTKTVQLTTEQKTALNISATANFILLGNKCLKLKTIHG", "aa_hexdigest": "ae026fa01f44486324269dbf017cbde2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6279.291399999998, "isoelectric_point": 9.361599159240722 }, "id": "KMADJIBOEG_236", "locus": "GCA000292445_00197" }, { "type": "cds", "sequence": "contig_1", "start": 104291, "stop": 104776, "strand": "+", "frame": 2, "gene": "oppC", "product": "Oligopeptide transport system permease protein OppC", "db_xrefs": [ "COG:COG1173", "COG:EP", "GO:0005886", "GO:0015031", "GO:0015833", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P75553", "UniRef:UniRef90_P47324" ], "nt": "ATGGATAGAAATAAAAGTTTTGACCCTAACTTATTTAAAAGGGTTGATATCAACTTATTAAAGCGAAATGATCAGCTTATTGGTAAACCAACTACCAATTCAATAGAAATTATCAAGCGCTTGTTTCAAAACAAGTGGGCCATCTTATTTTTTCTTTTAATAGTTGTTATTGTGCTATTAGCAATTATTGTGCCTTTAACTTCCCCTTTTTCAGCAGTAACTCCTGTTTCAACCAATGCCTTAGCACAAAATCTACCACCACGGTACTTATGGCATAAACCAGGTGACATTTTAGTTCATAAGATTACAGCAAGATCAATTGCTGAAATCTCTCAAGCTAGTGGAGTTTTAGTAGGAACATTACCTAGTGCAAATAGTAATCCCTTAGCAACTAATGTCCAGTATGATATTGCTCCTTTTCAACTCCAAGAATTGCGTAATTATTTCCCTTTATTGGGGACTAATGGACTTGGGATTGATATTTGA", "aa": "MDRNKSFDPNLFKRVDINLLKRNDQLIGKPTTNSIEIIKRLFQNKWAILFFLLIVVIVLLAIIVPLTSPFSAVTPVSTNALAQNLPPRYLWHKPGDILVHKITARSIAEISQASGVLVGTLPSANSNPLATNVQYDIAPFQLQELRNYFPLLGTNGLGIDI", "aa_hexdigest": "1b9067c6ad8d61f120903ceacc121ac9", "start_type": "ATG", "rbs_motif": null, "genes": [ "oppC" ], "truncated": "3-prime", "pseudogene": { "start": 104291, "stop": 105079, "observations": { "mutation": [ 104774, 104786, 104810, 104951, 105041, 105047 ], "stop": [ 104774, 104786, 104810, 104951, 105041, 105047 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47324", "query_cov": 1.0, "subject_cov": 0.42819148936170215, "identity": 1.0, "score": 301.0, "evalue": 2.66e-100, "gene_start": 1, "gene_end": 161, "reference_start": 1, "reference_end": 161, "reference_sequence": "MDRNKSFDPNLFKRVDINLLKRNDQLIGKPTTNSIEIIKRLFQNKWAILFFLLIVVIVLLAIIVPLTSPFSAVTPVSTNALAQNLPPRYLWHKPGDILVHKITARSIAEISQASGVLVGTLPSANSNPLATNVQYDIAPFQLQELRNYFPLLGTNGLGIDIWTLLWASVAKSLWIAVVVAIIAMVFGTIYGAVAGSFVGHMADNIMSRIIEIIDIVPSILWIIVLGATFRFGGVKQFDDSVVIFTLIFVFWTWPATTTRIYILKNKDTEYIQAAKTLGAHQIRIIFVHMLPVVFGRLAVVFVSLIPAVIGYEASLVFLGLKPATDIGLGALLNQVTSSDNVALILSSIVSFAVLTVAARTFANALNDAIDPRVVKR" }, "paralog": false, "identity": 0.9732824427480916, "score": 418.0, "evalue": 1.19e-148, "description": "Internal stop codon at 104774, 104786, 104810, 104951, 105041, 105047. Nonsense mutation around 104774, 104786, 104810, 104951, 105041, 105047.", "psc": { "uniref90_id": "UniRef90_P47324", "gene": "oppC", "product": "Oligopeptide transport system permease protein OppC", "uniref50_id": "UniRef50_P75553", "cog_id": "COG1173", "cog_category": "EP", "go_ids": [ "GO:0005886", "GO:0015833", "GO:0015031", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47324", "UniRef:UniRef50_P75553", "COG:COG1173", "COG:EP", "GO:0005886", "GO:0015833", "GO:0015031", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P75553", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75553" ], "product": "Oligopeptide transport system permease protein OppC" } }, "id": "KMADJIBOEG_237", "locus": "GCA000292445_00198" }, { "type": "cds", "sequence": "contig_1", "start": 104840, "stop": 104953, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGTTTGGAACCATTTATGGAGCGGTTGCTGGAAGCTTTGTTGGACATATGGCTGATAACATTATGAGTAGGATCATTGAGATTATTGATATAGTCCCTTCTATTCTTTGA", "aa": "MVFGTIYGAVAGSFVGHMADNIMSRIIEIIDIVPSIL", "aa_hexdigest": "4936c367a942cc35c6979e56c0d499a9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3951.6727999999994, "isoelectric_point": 4.535092353820801 }, "id": "KMADJIBOEG_238", "locus": "GCA000292445_00199" }, { "type": "cds", "sequence": "contig_1", "start": 105077, "stop": 105421, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAACAAAGATACAGAGTACATCCAAGCAGCTAAGACCTTAGGGGCACACCAAATCAGAATTATCTTTGTTCATATGTTACCTGTTGTATTTGGGAGATTAGCTGTTGTGTTTGTTAGTTTAATCCCAGCAGTTATTGGTTATGAAGCTTCCTTAGTTTTCCTTGGGTTAAAACCAGCTACTGATATTGGCTTAGGGGCACTTTTAAACCAAATAACTTCAAGTGATAATGTAGCTTTAATCTTAAGTTCGATTGTTAGCTTTGCAGTTTTAACAGTAGCAGCTAGAACATTTGCTAATGCTTTAAATGATGCAATTGACCCTAGGGTTGTAAAACGATAA", "aa": "MKNKDTEYIQAAKTLGAHQIRIIFVHMLPVVFGRLAVVFVSLIPAVIGYEASLVFLGLKPATDIGLGALLNQITSSDNVALILSSIVSFAVLTVAARTFANALNDAIDPRVVKR", "aa_hexdigest": "78ef30a7a32902437fedcea572798312", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12204.306299999978, "isoelectric_point": 9.59697322845459 }, "id": "KMADJIBOEG_239", "locus": "GCA000292445_00200" }, { "type": "cds", "sequence": "contig_1", "start": 105423, "stop": 106340, "strand": "+", "frame": 3, "gene": "oppD", "product": "Oligopeptide transport ATP-binding protein OppD", "db_xrefs": [ "COG:COG0444", "COG:EP", "EC:7.4.2.6", "GO:0005524", "GO:0005886", "GO:0015031", "GO:0015833", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P47325", "UniRef:UniRef90_P47325" ], "nt": "ATGGCACTTAAAAGAAGTAATTTCTTTGTTGATAAAGACCAACAACTAAAGGATAATTTGATCTTAGACATCACTGATTTACATGTTAACTTTAAGGTTAAAGATGGGATCTTACATGCTGTTAGAGGGATTGATCTTAAGGTAGAGAGGGGTAGTATTGTAGGGATTGTAGGTGAATCGGGCAGTGGTAAATCAGTGAGTGTTAAATCAATTATTGGTTTTAATGACAATGCACAAACTAAAGCCAAACTGATGAACTTTAAAAACGTTGATATTACCAAACTAAAGAAACACCAGTGGAAGTATTATAGAGGGACATATGTCTCTTATATTTCCCAAGACCCATTGTTTTCTCTAAACCCAACAATGACGATAGGAAAACAAGTAAAAGAAGCGATTTATGTGGCTTCAAAAAGAAGGTATTTCCAAGCTAAATCAGACTTAAAATTTGCTTTATCAAATAAGGAGATTGACAAAAAAACTTATAAAAGTAAACTAAAAGAGATCAAACAAACCTACCAACAAAAAATAAAACCTATCAATGTAGAGAAAAAAACCTTAGAGATCCTGCAGTTCATTGGTATTAATGATGCCAAGAAACGTTTAAAGGCATTCCCAAGTGAGTTTTCAGGAGGGATGAGACAGAGAATTGTGATTGCTATTGCAGTAGCAACTGAACCTGATTTAATTATTGCTGATGAACCTACTACTGCACTTGATGTAACTATTCAAGCTAAGGTATTAACTTTAATTAAACAACTCCGTGATCTACTTAATATCACTATTATCTTTATTAGTCACAATATCTCTTTAATTGCTAATTTCTGTGACTTTGTTTATGTTATGTATGCAGGAAAAATTGTAGAACAGGGTCTGGTTGAAGAGATCTTTACAAATCCACTCCATCCCTATACATGA", "aa": "MALKRSNFFVDKDQQLKDNLILDITDLHVNFKVKDGILHAVRGIDLKVERGSIVGIVGESGSGKSVSVKSIIGFNDNAQTKAKLMNFKNVDITKLKKHQWKYYRGTYVSYISQDPLFSLNPTMTIGKQVKEAIYVASKRRYFQAKSDLKFALSNKEIDKKTYKSKLKEIKQTYQQKIKPINVEKKTLEILQFIGINDAKKRLKAFPSEFSGGMRQRIVIAIAVATEPDLIIADEPTTALDVTIQAKVLTLIKQLRDLLNITIIFISHNISLIANFCDFVYVMYAGKIVEQGLVEEIFTNPLHPYT", "aa_hexdigest": "aeb14029ceee6c6c76cfd8b2c8d1ca04", "start_type": "ATG", "rbs_motif": null, "genes": [ "oppD" ], "truncated": "3-prime", "pseudogene": { "start": 105423, "stop": 106631, "observations": { "mutation": [ 106338, 106533 ], "stop": [ 106338, 106533 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47325", "query_cov": 1.0, "subject_cov": 0.7587064676616916, "identity": 1.0, "score": 575.0, "evalue": 3.51e-205, "gene_start": 1, "gene_end": 305, "reference_start": 1, "reference_end": 305, "reference_sequence": "MALKRSNFFVDKDQQLKDNLILDITDLHVNFKVKDGILHAVRGIDLKVERGSIVGIVGESGSGKSVSVKSIIGFNDNAQTKAKLMNFKNVDITKLKKHQWKYYRGTYVSYISQDPLFSLNPTMTIGKQVKEAIYVASKRRYFQAKSDLKFALSNKEIDKKTYKSKLKEIKQTYQQKIKPINVEKKTLEILQFIGINDAKKRLKAFPSEFSGGMRQRIVIAIAVATEPDLIIADEPTTALDVTIQAKVLTLIKQLRDLLNITIIFISHNISLIANFCDFVYVMYAGKIVEQGLVEEIFTNPLHPYTWALISSIPEQKDKNKPLTSIPGVIPNMLTPPKGDAFASRNQYALAIDFEYHPPFFEVTKTHKAATWLLHPQAPKVEPPQAVIDNITLTKKALQFKDQ" }, "paralog": false, "identity": 0.9950248756218906, "score": 782.0, "evalue": 1.98e-289, "description": "Internal stop codon at 106338, 106533. Nonsense mutation around 106338, 106533.", "psc": { "uniref90_id": "UniRef90_P47325", "gene": "oppD", "product": "Oligopeptide transport ATP-binding protein OppD", "ec_ids": [ "7.4.2.6" ], "uniref50_id": "UniRef50_P47325", "cog_id": "COG0444", "cog_category": "EP", "go_ids": [ "GO:0005886", "GO:0005524", "GO:0016887", "GO:0015833", "GO:0015031" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47325", "EC:7.4.2.6", "UniRef:UniRef50_P47325", "COG:COG0444", "COG:EP", "GO:0005886", "GO:0005524", "GO:0016887", "GO:0015833", "GO:0015031" ] }, "pscc": { "uniref50_id": "UniRef50_P47325", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47325" ], "product": "Oligopeptide transport ATP-binding protein OppD" } }, "id": "KMADJIBOEG_240", "locus": "GCA000292445_00201" }, { "type": "cds", "sequence": "contig_1", "start": 106344, "stop": 106535, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTCTTCAATTCCTGAACAGAAAGATAAAAACAAGCCACTAACTTCTATCCCTGGAGTTATTCCTAACATGTTAACCCCACCAAAGGGTGATGCTTTCGCTAGCAGAAACCAATATGCTCTAGCAATTGACTTTGAATACCATCCACCCTTTTTTGAAGTTACTAAAACCCATAAAGCAGCAACCTGA", "aa": "MISSIPEQKDKNKPLTSIPGVIPNMLTPPKGDAFASRNQYALAIDFEYHPPFFEVTKTHKAAT", "aa_hexdigest": "880389c4dace7ba724a7d915fb089c58", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7000.960500000002, "isoelectric_point": 8.076164054870606 }, "id": "KMADJIBOEG_241", "locus": "GCA000292445_00202" }, { "type": "cds", "sequence": "contig_1", "start": 106631, "stop": 107980, "strand": "+", "frame": 2, "gene": "oppF", "product": "Oligopeptide transport ATP-binding protein OppF", "db_xrefs": [ "COG:COG4172", "COG:Q", "EC:7.4.2.6", "GO:0005524", "GO:0005886", "GO:0015031", "GO:0015833", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P75551", "UniRef:UniRef90_P47326" ], "nt": "ATGGAAAACCAAAACACAAAAAAACCACTTGTTAATGTTAAGGCTTTGAGCATGATGTTCAAGGTCAGAGGAACTCTTTTTAAAGCCCTTGATGAAATTGGTTTTACTGTTAATGAAGGGGACTTCTTTGGGGTTATTGGTGAGAGTGGTAGTGGTAAATCAACCACGGGAAAATGTTTGATTAGATTAAACATTCCTAGTGGTGGAAAGATTGAGATTGCCAACCACTTACTCTCAGGAAAAAAACTTACTAAAGAGAATAACCAGTGGTTAAAACAAAACATCCAAATGGTGTTTCAAGACCCTTATTCATCTATTAACCCTACTAAAAATGTGCTAACTGTGATTTCAGAACCGCTGGTAATTAGTAAAACTGTTTTTGGGGAAACAAAACAATACTTAAAGAGTTTGCAAAAGCTCTCTTTTAAAGTAAAGAAAACATTGTTAAGGAATGATATTGAACTTGAAACCAAGTTTCACAATAACTTTTTTAAAACCGTTATTAAGCAAATTAATGAATCATTGTTTAACTTTGAAGATCTTGATTACAAGGATTTAAAACCATCACATTTAAGGCAAAGAATCATAAATGAAACAGATAAATTCATTGAAAAAATTAGAAGTGAGTTTGCCCTTTTTTATGATTTTTATGCTAACCAATCAGTACCCTTGCAAAAGGCATTAGATGATGCGAATTCCTCTTTAACACCATCTAGTGTTATTGAGTTAAAAAACCAGTTAAAAGCATTACAAAAACAAGCAAAGATTTCAAAGGCAGCATGGGATATTTTACAAGCCCTAAAGCAAAACCAAAAGGAGTTGAAAGATTATGAAAACTATGTCCATTTTGAACTCCAAAAAAAGCCACGAATCTATCTTAATACCTGGCTTTTAACAACCAAAAGCTACATTAAAGATTCCAAGCAAAACATGCAGCTTACTGATGATATCTTTGCTTTTTCATACAACAGTATGGTTGACAAGAAAAGAAACTTGGTTTTAATTCTTTCTAAATACTATAAGCTGTTACCTTATTTCTATGACCAATCAGTATTTGATAATGCTGATCAATTTGATGAAATTGCTAACCTTATCTTTTTTGATTTAGTTGAAACATTGCTTGGTGTAACTAGTTTATTTAATGATGCATTAGCAGCTGATAAAGTCCCACTAATTAAGTTTGCTAAGTTCTTAAATAAGTTATGTGACTTGCGCTTTTTAACCTTAAAAAAGAGCTTTAAAAAAACAAGAGTAAGTTGTAGCTTTAGTTTTAACAGTGAACCTGAAATCTTGTTTGCCAACAGCTGCTATGATTTGCAACAAATGCCTCAAATCATTAAACCCTTTTGA", "aa": "MENQNTKKPLVNVKALSMMFKVRGTLFKALDEIGFTVNEGDFFGVIGESGSGKSTTGKCLIRLNIPSGGKIEIANHLLSGKKLTKENNQWLKQNIQMVFQDPYSSINPTKNVLTVISEPLVISKTVFGETKQYLKSLQKLSFKVKKTLLRNDIELETKFHNNFFKTVIKQINESLFNFEDLDYKDLKPSHLRQRIINETDKFIEKIRSEFALFYDFYANQSVPLQKALDDANSSLTPSSVIELKNQLKALQKQAKISKAAWDILQALKQNQKELKDYENYVHFELQKKPRIYLNTWLLTTKSYIKDSKQNMQLTDDIFAFSYNSMVDKKRNLVLILSKYYKLLPYFYDQSVFDNADQFDEIANLIFFDLVETLLGVTSLFNDALAADKVPLIKFAKFLNKLCDLRFLTLKKSFKKTRVSCSFSFNSEPEILFANSCYDLQQMPQIIKPF", "aa_hexdigest": "e296e99498ba69c322aaa62c9ce285d6", "start_type": "ATG", "rbs_motif": null, "genes": [ "oppF" ], "truncated": "3-prime", "pseudogene": { "start": 106631, "stop": 108226, "observations": { "mutation": [ 107978 ], "stop": [ 107978 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47326", "query_cov": 1.0, "subject_cov": 0.5294811320754716, "identity": 1.0, "score": 868.0, "evalue": 8.27e-312, "gene_start": 1, "gene_end": 449, "reference_start": 1, "reference_end": 449, "reference_sequence": "MENQNTKKPLVNVKALSMMFKVRGTLFKALDEIGFTVNEGDFFGVIGESGSGKSTTGKCLIRLNIPSGGKIEIANHLLSGKKLTKENNQWLKQNIQMVFQDPYSSINPTKNVLTVISEPLVISKTVFGETKQYLKSLQKLSFKVKKTLLRNDIELETKFHNNFFKTVIKQINESLFNFEDLDYKDLKPSHLRQRIINETDKFIEKIRSEFALFYDFYANQSVPLQKALDDANSSLTPSSVIELKNQLKALQKQAKISKAAWDILQALKQNQKELKDYENYVHFELQKKPRIYLNTWLLTTKSYIKDSKQNMQLTDDIFAFSYNSMVDKKRNLVLILSKYYKLLPYFYDQSVFDNADQFDEIANLIFFDLVETLLGVTSLFNDALAADKVPLIKFAKFLNKLCDLRFLTLKKSFKKTRVSCSFSFNSEPEILFANSCYDLQQMPQIIKPFWEKLFNEQNYQKIIDSVSRLNVMIANYITKAFEIKKTIDEKLREFKQQNLALKKAYSANKKSEANKASINELKVNLKTLKKQLKQEKNTTKKQSKKELKPLLKEHHTALKLHDEFNHDLRKWFKKLNFMVKKYNRLENSQKKFCLVKKLKALFKKQDETLQSELRPKLKTFGVINFEYKRAVKESNVFRLVHFAKNIFKPFLFFNLTKIFMRNKVYEALDSVGLKREHAYRYPHEFSGGQRQRIAIARALITKPKLIIADELISALDVSIQAQVINILKDLAKKHNLTVLFIAHDLSMVQTVCNRLIIMHRGKIVERGSVDEIFSNPVHPYTRSLIKASPKLSKINVDLASFDENFTYDSDYSLTNMPFYIKVPNSEEHELYCTQKQFDSWIKEATPIN" }, "paralog": false, "identity": 0.9962335216572504, "score": 1034.0, "evalue": 0.0, "description": "Internal stop codon at 107978. Nonsense mutation around 107978.", "psc": { "uniref90_id": "UniRef90_P47326", "gene": "oppF", "product": "Oligopeptide transport ATP-binding protein OppF", "ec_ids": [ "7.4.2.6" ], "uniref50_id": "UniRef50_P75551", "cog_id": "COG4172", "cog_category": "Q", "go_ids": [ "GO:0005886", "GO:0005524", "GO:0016887", "GO:0015833", "GO:0015031" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47326", "EC:7.4.2.6", "UniRef:UniRef50_P75551", "COG:COG4172", "COG:Q", "GO:0005886", "GO:0005524", "GO:0016887", "GO:0015833", "GO:0015031" ] }, "pscc": { "uniref50_id": "UniRef50_P75551", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75551" ], "product": "Oligopeptide transport ATP-binding protein OppF" } }, "id": "KMADJIBOEG_242", "locus": "GCA000292445_00203" }, { "type": "cds", "sequence": "contig_1", "start": 108044, "stop": 109150, "strand": "+", "frame": 2, "gene": "oppF", "product": "Oligopeptide transport ATP-binding protein OppF", "db_xrefs": [ "COG:COG4172", "COG:Q", "EC:7.4.2.6", "GO:0005524", "GO:0005886", "GO:0015031", "GO:0015833", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P75551", "UniRef:UniRef90_P47326" ], "nt": "ATGATTGCAAATGACATTACCAAAGCTTTTGAAATTAAAAAAACTATTGATGAAAAACTAAGGGAGTTTAAACAACAAAATTTAGCTTTAAAAAAAGCTTATTCAGCTAACAAGAAAAGTGAGGCAAACAAAGCTTCCATTAATGAGTTAAAAGTCAATTTAAAAACACTTAAAAAACAGCTTAAACAAGAGAAAAATACTACTAAAAAACAATCAAAAAAGGAATTAAAACCACTTTTAAAAGAACACCATACTGCTTTAAAACTCCATGATGAGTTTAACCATGATTTACGCAAGTGGTTCAAAAAACTTAACTTTATGGTTAAGAAATACAACCGACTGGAAAACAGCCAGAAAAAGTTTTGTTTAGTTAAAAAGTTAAAAGCGCTTTTCAAAAAACAGGATGAAACACTACAAAGTGAATTAAGACCAAAACTAAAAACATTTGGTGTAATTAACTTTGAGTACAAACGTGCAGTCAAAGAGTCCAATGTCTTTCGATTGGTGCATTTTGCTAAAAATATCTTTAAACCATTCTTGTTTTTTAACCTCACCAAGATTTTTATGAGAAATAAGGTCTATGAAGCACTCGATAGTGTTGGTTTAAAAAGAGAACATGCTTACAGATACCCCCATGAATTTTCAGGCGGACAAAGACAAAGAATTGCTATTGCCCGTGCTTTAATCACTAAACCCAAACTGATTATTGCAGATGAATTGATTAGTGCACTTGATGTTTCTATCCAAGCCCAAGTTATTAACATCTTGAAAGACTTGGCTAAAAAACACAACTTAACTGTGCTTTTCATTGCCCATGATTTATCAATGGTGCAAACTGTTTGTAACCGTTTGATCATTATGCATAGGGGCAAGATTGTTGAACGGGGCAGTGTGGATGAGATCTTTTCAAATCCAGTTCATCCCTACACCCGTTCCCTAATAAAAGCATCTCCTAAGTTAAGCAAAATCAATGTTGATCTCGCTTCTTTTGATGAAAACTTCACTTATGATAGTGATTATTCACTAACCAATATGCCCTTTTATATTAAAGTTCCTAACAGTGAAGAACATGAACTTTACTGTACTCAAAAGCAATTTGATAGTTGA", "aa": "MIANDITKAFEIKKTIDEKLREFKQQNLALKKAYSANKKSEANKASINELKVNLKTLKKQLKQEKNTTKKQSKKELKPLLKEHHTALKLHDEFNHDLRKWFKKLNFMVKKYNRLENSQKKFCLVKKLKALFKKQDETLQSELRPKLKTFGVINFEYKRAVKESNVFRLVHFAKNIFKPFLFFNLTKIFMRNKVYEALDSVGLKREHAYRYPHEFSGGQRQRIAIARALITKPKLIIADELISALDVSIQAQVINILKDLAKKHNLTVLFIAHDLSMVQTVCNRLIIMHRGKIVERGSVDEIFSNPVHPYTRSLIKASPKLSKINVDLASFDENFTYDSDYSLTNMPFYIKVPNSEEHELYCTQKQFDS", "aa_hexdigest": "a5a364007d82da4152f4b0662b353388", "start_type": "ATG", "rbs_motif": null, "genes": [ "oppF" ], "truncated": "both", "pseudogene": { "start": 107744, "stop": 109177, "observations": { "mutation": [ 107978, 109148 ], "stop": [ 107978, 109148 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47326", "query_cov": 1.0, "subject_cov": 0.4339622641509434, "identity": 0.997, "score": 652.0, "evalue": 5.59e-228, "gene_start": 1, "gene_end": 368, "reference_start": 472, "reference_end": 839, "reference_sequence": "MENQNTKKPLVNVKALSMMFKVRGTLFKALDEIGFTVNEGDFFGVIGESGSGKSTTGKCLIRLNIPSGGKIEIANHLLSGKKLTKENNQWLKQNIQMVFQDPYSSINPTKNVLTVISEPLVISKTVFGETKQYLKSLQKLSFKVKKTLLRNDIELETKFHNNFFKTVIKQINESLFNFEDLDYKDLKPSHLRQRIINETDKFIEKIRSEFALFYDFYANQSVPLQKALDDANSSLTPSSVIELKNQLKALQKQAKISKAAWDILQALKQNQKELKDYENYVHFELQKKPRIYLNTWLLTTKSYIKDSKQNMQLTDDIFAFSYNSMVDKKRNLVLILSKYYKLLPYFYDQSVFDNADQFDEIANLIFFDLVETLLGVTSLFNDALAADKVPLIKFAKFLNKLCDLRFLTLKKSFKKTRVSCSFSFNSEPEILFANSCYDLQQMPQIIKPFWEKLFNEQNYQKIIDSVSRLNVMIANYITKAFEIKKTIDEKLREFKQQNLALKKAYSANKKSEANKASINELKVNLKTLKKQLKQEKNTTKKQSKKELKPLLKEHHTALKLHDEFNHDLRKWFKKLNFMVKKYNRLENSQKKFCLVKKLKALFKKQDETLQSELRPKLKTFGVINFEYKRAVKESNVFRLVHFAKNIFKPFLFFNLTKIFMRNKVYEALDSVGLKREHAYRYPHEFSGGQRQRIAIARALITKPKLIIADELISALDVSIQAQVINILKDLAKKHNLTVLFIAHDLSMVQTVCNRLIIMHRGKIVERGSVDEIFSNPVHPYTRSLIKASPKLSKINVDLASFDENFTYDSDYSLTNMPFYIKVPNSEEHELYCTQKQFDSWIKEATPIN" }, "paralog": false, "identity": 0.9937106918238994, "score": 881.0, "evalue": 1.58e-320, "description": "Internal stop codon at 107978, 109148. Nonsense mutation around 107978, 109148.", "psc": { "uniref90_id": "UniRef90_P47326", "gene": "oppF", "product": "Oligopeptide transport ATP-binding protein OppF", "ec_ids": [ "7.4.2.6" ], "uniref50_id": "UniRef50_P75551", "cog_id": "COG4172", "cog_category": "Q", "go_ids": [ "GO:0005886", "GO:0005524", "GO:0016887", "GO:0015833", "GO:0015031" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47326", "EC:7.4.2.6", "UniRef:UniRef50_P75551", "COG:COG4172", "COG:Q", "GO:0005886", "GO:0005524", "GO:0016887", "GO:0015833", "GO:0015031" ] }, "pscc": { "uniref50_id": "UniRef50_P75551", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75551" ], "product": "Oligopeptide transport ATP-binding protein OppF" } }, "id": "KMADJIBOEG_243", "locus": "GCA000292445_00204" }, { "type": "cds", "sequence": "contig_1", "start": 109233, "stop": 109646, "strand": "+", "frame": 3, "gene": "rplK", "product": "50S ribosomal protein L11", "db_xrefs": [ "COG:COG0080", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:0070180", "KEGG:K02867", "RefSeq:WP_009885638.1", "SO:0001217", "UniParc:UPI0000165F74", "UniRef:UniRef100_P47327", "UniRef:UniRef50_P47327", "UniRef:UniRef90_P47327" ], "nt": "ATGGCTAAAAAAACAGTTACAAGAATCGCTAAGATTAACCTAATTGGCGGACAAGCAAAACCTGGCCCTGCGCTTGCTTCTGTAGGGATTAATATGGGTGAGTTCACCAAACAATTTAATGAAAAAACCAAGGATAGACAAGGTGAAACGATCCCTTGTATAATCACTGCTTTTAACGATAAATCATTTACTTTTGTCTTAAAAACTACCCCTGTTAGCAACTTAATTAAACAGGCTGCTAAACTAGAAAAAGGTGCTAAAAATGCAAAAACTATTGTTGGAAAAATCTCCTTACAACAAGCTAAGGAGATTGCGCAATACAAGTTAGTTGATCTTAATGCTAACACAGTTGAAGCAGCATTAAAAATGGTGTTAGGTACAGCTAAACAGATGGGAATAGAGGTAACTGATTAA", "aa": "MAKKTVTRIAKINLIGGQAKPGPALASVGINMGEFTKQFNEKTKDRQGETIPCIITAFNDKSFTFVLKTTPVSNLIKQAAKLEKGAKNAKTIVGKISLQQAKEIAQYKLVDLNANTVEAALKMVLGTAKQMGIEVTD", "aa_hexdigest": "19bb1803ec9bd00a603f99a2e03a8a04", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000165F74", "ncbi_nrp_id": "WP_009885638.1", "uniref100_id": "UniRef100_P47327", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000165F74", "RefSeq:WP_009885638.1", "UniRef:UniRef100_P47327" ] }, "ips": { "uniref100_id": "UniRef100_P47327", "uniref90_id": "UniRef90_P47327" }, "psc": { "uniref90_id": "UniRef90_P47327", "gene": "rplK", "product": "50S ribosomal protein L11", "uniref50_id": "UniRef50_P47327", "cog_id": "COG0080", "cog_category": "J", "kegg_orthology_id": "K02867", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:0070180" ] }, "pscc": { "uniref50_id": "UniRef50_P47327", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47327" ], "product": "Large ribosomal subunit protein uL11" }, "genes": [ "rplK" ], "id": "KMADJIBOEG_244", "locus": "GCA000292445_00205" }, { "type": "cds", "sequence": "contig_1", "start": 109646, "stop": 109789, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACTATCAAAAAAGATGCAAGCTGTTACTAAGCTCATTGATAAAAACAAACTTTATCCTATCCAAGAAGCATTTGAATTAATTAAAAAACAGCAATTACTAAGTTTGTCAGTTCAGTTGATATTGCTGTTAGTTTAA", "aa": "MKKLSKKMQAVTKLIDKNKLYPIQEAFELIKKQQLLSLSVQLILLLV", "aa_hexdigest": "bb768e183bae3d04a163adbfb5f32766", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5468.7328, "isoelectric_point": 10.000158500671386 }, "id": "KMADJIBOEG_245", "locus": "GCA000292445_00206" }, { "type": "cds", "sequence": "contig_1", "start": 109999, "stop": 110325, "strand": "+", "frame": 1, "gene": null, "product": "Ribosomal protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2P6FDS1" ], "nt": "ATGGGAGCATTAGGTAAACTAGGAAAACTATTAGGAACCAGGGGATTGATGCCAAACCCAAAAACTGAAACAGTTACTGATGATGTAGTTAGTGCTATTAAAGCTTATAAAAAGGGTAAGAAAGAATATCGAACTGATTCATTTGGTAACATCCACCTCTCTTTAGGTAAAACAGATACCAAAACTGAGCACTTGGTTGCTAATGCCATGGCTTTAATAGATTTAATTAAATCTAAACGTCCTAGCACAGTCAAAGGTACTTACATTAAAAATATTGCTTTGACAACAACAATGGGACCAAGTTTAAAAGTAAAGCTACCTGATTAA", "aa": "MGALGKLGKLLGTRGLMPNPKTETVTDDVVSAIKAYKKGKKEYRTDSFGNIHLSLGKTDTKTEHLVANAMALIDLIKSKRPSTVKGTYIKNIALTTTMGPSLKVKLPD", "aa_hexdigest": "4256e6580f77ad810c76182ec7135e66", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2P6FDS1", "query_cov": 0.9629629629629629, "subject_cov": 0.9629629629629629, "identity": 0.5870000000000001, "score": 117.0, "evalue": 5.77e-32, "valid": false, "product": "Ribosomal protein", "uniref50_id": "UniRef50_A0A2P6FDS1", "kegg_orthology_id": "K02863" }, "pscc": { "uniref50_id": "UniRef50_A0A2P6FDS1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2P6FDS1" ], "product": "Ribosomal protein" }, "genes": [], "id": "KMADJIBOEG_246", "locus": "GCA000292445_00207" }, { "type": "cds", "sequence": "contig_1", "start": 110325, "stop": 110894, "strand": "+", "frame": 3, "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "db_xrefs": [ "COG:COG0193", "COG:J", "EC:3.1.1.29", "GO:0004045", "GO:0005737", "GO:0006412", "RefSeq:WP_009885642.1", "SO:0001217", "UniParc:UPI0000132923", "UniRef:UniRef100_P47329", "UniRef:UniRef50_P47329", "UniRef:UniRef90_P47329" ], "nt": "ATGCCCACCTATAAACTAATTGTTGGTTTAGGTAACTTAGGTAAAAAGTATGAGAAAACCCGCCATAATGCTGGTTTTATGGTATTAGATAGACTAGCTAGTTTATTCCACTTAAACTTTGATAAAACCAACAAGTTAGGTGATTATCTTTTTATTAAAGAAAAAGCAGCAATCTTAGCAAAACCTGCTACCTTTATGAATAATAGCGGTCTTTTTGTGAAATGGTTACAAGATCACTTTCAAATTCCGCTTGCAAACATAATGATAGTCCATGATGAAATAGCGTTTGATTTGGGAGTAATTAGGCTTAAAATGCAAGGGAGTGCTAACAATCATAATGGCATAAAATCAGTAATTAGACATTTAGATACTGAACAGTTCAATCGTTTACGCTTTGGGATTAAATCACAAAATACGAGTAACATATTGCATGAACAGGTAATGAGTGAATTCCAGAATAGTGAACTGACTAAACTGGAAGTTGCGATTACAAAGTCTGTTGAACTGTTGAAGCGTTATATTGAAGGAGAAGAGTTACAAAGGTTAATGGAATATTATCATCATGGCTAG", "aa": "MPTYKLIVGLGNLGKKYEKTRHNAGFMVLDRLASLFHLNFDKTNKLGDYLFIKEKAAILAKPATFMNNSGLFVKWLQDHFQIPLANIMIVHDEIAFDLGVIRLKMQGSANNHNGIKSVIRHLDTEQFNRLRFGIKSQNTSNILHEQVMSEFQNSELTKLEVAITKSVELLKRYIEGEELQRLMEYYHHG", "aa_hexdigest": "9408931b37249c9e048343d2b12aa2c3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000132923", "ncbi_nrp_id": "WP_009885642.1", "uniref100_id": "UniRef100_P47329", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000132923", "RefSeq:WP_009885642.1", "UniRef:UniRef100_P47329" ] }, "ips": { "uniref100_id": "UniRef100_P47329", "uniref90_id": "UniRef90_P47329" }, "psc": { "uniref90_id": "UniRef90_P47329", "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "ec_ids": [ "3.1.1.29" ], "uniref50_id": "UniRef50_P47329", "cog_id": "COG0193", "cog_category": "J", "go_ids": [ "GO:0004045", "GO:0005737", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P47329", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47329" ], "product": "Peptidyl-tRNA hydrolase" }, "genes": [ "pth" ], "id": "KMADJIBOEG_247", "locus": "GCA000292445_00208" }, { "type": "cds", "sequence": "contig_1", "start": 110887, "stop": 111099, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAGTGAAAAACAATATATAGCAGGGGTTTCAGGTGGATCTGATTCAATGCTAATGCTTAAACTTTACCAAAAGAAGATTGCTTGTGTTGTTCATGTCAATTACAACACCAGATCAACCTCATTACGTGATCAAAAGTTAGTAGAACAATATTGTCAAAAATTAAATATTCCACTGGTTGTTCACACTGTTGATCCTGATCTAGTTTGA", "aa": "MASEKQYIAGVSGGSDSMLMLKLYQKKIACVVHVNYNTRSTSLRDQKLVEQYCQKLNIPLVVHTVDPDLV", "aa_hexdigest": "9bcf35473bb65cd8ff84750fd09c6bd0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7869.1032000000005, "isoelectric_point": 8.640069007873539 }, "id": "KMADJIBOEG_248", "locus": "GCA000292445_00209" }, { "type": "cds", "sequence": "contig_1", "start": 111226, "stop": 111324, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGCTGGATGCAAAAAAACGTGCTGTTTACTATGGGATTAAAACCAGGTGTGAATTGTATGGTTTGAAAATCTACCGTCCCCTAATGAAATACTGA", "aa": "MQLDAKKRAVYYGIKTRCELYGLKIYRPLMKY", "aa_hexdigest": "349c372203ed58aa655e24dde8590348", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3913.719500000002, "isoelectric_point": 9.843049049377441 }, "id": "KMADJIBOEG_249", "locus": "GCA000292445_00210" }, { "type": "cds", "sequence": "contig_1", "start": 111415, "stop": 111759, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGGTTAGAGATAGAAAAATGGTCTAAAATCGAAAAAGAACAGTTTTATATTGCTATCTGTGCAATGAACAAAACAATTGCTCAAAAACTGTTTGTATTAATGAAAAAAGCTAAAAAATGGTTATTACAACCTGATGTTAGAGAATTGAAACGGTTTTCAATTATTGATCAAAAACAGTTAATTTATAGCTATCTTATCTATCACAAGATTAATGTTAATGGTGAGAAAATTGATGCTATCCTTGATTTTATCCAACCTAGTCAACAAAAACAATACCGCTTGCAAAACGATATCTTTTTAATGGTGAAAAACCAGTGTTTAGCACTTTTATACAAAAGCTAA", "aa": "MRLEIEKWSKIEKEQFYIAICAMNKTIAQKLFVLMKKAKKWLLQPDVRELKRFSIIDQKQLIYSYLIYHKINVNGEKIDAILDFIQPSQQKQYRLQNDIFLMVKNQCLALLYKS", "aa_hexdigest": "e11f65335f95187cde31ac200bc29b48", "start_type": "GTG", "rbs_motif": "ATTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13758.283499999985, "isoelectric_point": 9.611091804504394 }, "id": "KMADJIBOEG_250", "locus": "GCA000292445_00211" }, { "type": "cds", "sequence": "contig_1", "start": 111760, "stop": 111999, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACATTTAACTGTCAAAGCATTAGTTTTGCAATTTAATGATTGTATCCAACTCATTGATGGTAAAAACAACATAGATAATGTGATTACTATCCCTGGATTAAAAAGGAGTGTTTTTGAACTGTTAGGGCTATTTTGTAAGCCAATTGGTTCAGTTGCTATTTTAGGCAAACGTGAATTTATTTTTTTAAATCAAAAGCCAGTTGAGCAACAGAAAAAATTATTGCAAACCTCTTGA", "aa": "MKHLTVKALVLQFNDCIQLIDGKNNIDNVITIPGLKRSVFELLGLFCKPIGSVAILGKREFIFLNQKPVEQQKKLLQTS", "aa_hexdigest": "248f7537e62ff57381cc0586b12d0d1e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8907.559399999993, "isoelectric_point": 9.566221809387208 }, "id": "KMADJIBOEG_251", "locus": "GCA000292445_00212" }, { "type": "cds", "sequence": "contig_1", "start": 111996, "stop": 112694, "strand": "+", "frame": 3, "gene": "hprK", "product": "HPr(Ser) kinase/phosphatase", "db_xrefs": [ "COG:COG1493", "COG:T", "EC:2.7.11.-", "EC:2.7.4.-", "GO:0000155", "GO:0000160", "GO:0000287", "GO:0004674", "GO:0004712", "GO:0005524", "GO:0005829", "GO:0006109", "KEGG:K06023", "SO:0001217", "UniRef:UniRef50_P75548", "UniRef:UniRef90_P47331" ], "nt": "TTGAAGCTCAAACCACCTGCAGTTATTCTAACCAAGTCATTTGTTGATTGTGGTGTTTTGTTAGCTGTTAATCAAACTTATCAAGTTCCTATTTTAAAAACTAACTTGTTTTCAACTGAACTCTCTTTTACTGTTGAAACCTATATTAACGAGCAATTTGCTACTGTTCAAAAGTTACATGGAGTTTTACTTGAAATCTTTGGTGTTGGGGTATTTTTAGAGGGAAAGAGTGGGATTGGTAAATCTGAAAGTGCTTTAGATTTAATTAATAAAAACCACCTTCTAATAGGGGATGATGCTGTTGAGATCTACAGATTAGGCAATAGGTTATTTGGTAGAGCACAAGCACTGGCAAAAGGCTTTATGGAGATTAGGGGTCTAGGCATCATTAATATTGAACGTGCTTATGGGTTACAGATTACAAAAGAACAAACTGAAATCCAACTAGCAATTAGTTTGTTGAGTTTAGAGGAGAAAAACAACGCTAGTTTTGAGCGCTTAGGCAGTGATTTAAAACTAAAAAATCTGCTTGGGGTTAAGATTAGTTACTATCAGATCCCTATCTCTTCAGGTAGAAAAACAAGCAAAATTATTGAAAGTGCAGTAATCGATTTTAAACTTAAGAAAAGTGGTTACAATTCAGCAAACGAGTTTATCTTAAAGCAACGTGCCATGTTAGAAGAACAAACTGATGAATAG", "aa": "MKLKPPAVILTKSFVDCGVLLAVNQTYQVPILKTNLFSTELSFTVETYINEQFATVQKLHGVLLEIFGVGVFLEGKSGIGKSESALDLINKNHLLIGDDAVEIYRLGNRLFGRAQALAKGFMEIRGLGIINIERAYGLQITKEQTEIQLAISLLSLEEKNNASFERLGSDLKLKNLLGVKISYYQIPISSGRKTSKIIESAVIDFKLKKSGYNSANEFILKQRAMLEEQTDE", "aa_hexdigest": "2189e8c47630dfb4b539615ed5a4bdc4", "start_type": "TTG", "rbs_motif": null, "genes": [ "hprK" ], "truncated": "5-prime", "pseudogene": { "start": 111760, "stop": 112694, "observations": { "deletion": [ 111979 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47331", "query_cov": 1.0, "subject_cov": 0.7459807073954984, "identity": 0.983, "score": 434.0, "evalue": 1.88e-152, "gene_start": 1, "gene_end": 232, "reference_start": 80, "reference_end": 311, "reference_sequence": "MKHLTVKALVLQFNDCIQLIDGKNNIDNVITIPGLKRSVFELLGLFCKPIGSVAILGKREFIFLNQKPVEQQKKIIANLLKLKPPAVILTKSFLDCGVLLAVNQTYQVPILKTNLFSTELSFTVETYINEQFATVQKLHGVLLEIFGVGVFLEGKSGIGKSESALDLINKNHLLIGDDAIEIYRLGNRLFGRAQALAKGFMEIRGLGIINIERAYGLQITKEQTEIQLAISLLSLEEKNNASFERLGSDLKLKNLLGVKISYYQIPISSGRKTSEIIESAVIDFKLKKSGYNSANEFILKQRAMLEEQTDE" }, "paralog": false, "identity": 0.9903536977491961, "score": 588.0, "evalue": 1.06e-215, "description": "Frameshift due to deletion around 111979.", "psc": { "uniref90_id": "UniRef90_P47331", "gene": "hprK", "product": "HPr(Ser) kinase/phosphatase", "ec_ids": [ "2.7.11.-", "2.7.4.-" ], "uniref50_id": "UniRef50_P75548", "cog_id": "COG1493", "cog_category": "T", "kegg_orthology_id": "K06023", "go_ids": [ "GO:0000155", "GO:0000160", "GO:0000287", "GO:0004674", "GO:0004712", "GO:0005524", "GO:0005829", "GO:0006109" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47331", "EC:2.7.11.-", "EC:2.7.4.-", "UniRef:UniRef50_P75548", "COG:COG1493", "COG:T", "KEGG:K06023", "GO:0000155", "GO:0000160", "GO:0000287", "GO:0004674", "GO:0004712", "GO:0005524", "GO:0005829", "GO:0006109" ] }, "pscc": { "uniref50_id": "UniRef50_P75548", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75548" ], "product": "HPr kinase/phosphorylase" } }, "id": "KMADJIBOEG_252", "locus": "GCA000292445_00213" }, { "type": "cds", "sequence": "contig_1", "start": 112789, "stop": 113595, "strand": "+", "frame": 1, "gene": "lgt", "product": "prolipoprotein diacylglyceryl transferase", "db_xrefs": [ "COG:COG0682", "COG:M", "EC:2.5.1.145", "GO:0005886", "GO:0008961", "GO:0009249", "GO:0016020", "GO:0042158", "SO:0001217", "UniRef:UniRef50_P47332", "UniRef:UniRef90_P47332" ], "nt": "ATGTTTGTCTTTAAACTGATTTATTGTTACAAATTACAAGACAACAGTTTTTATTTTTTTATCTTTATTGCTGTTTTAACGATGGTTTTAGGCGCTCGCCTCTGGTCATTTGTAATTGGTGATTCCAATTTTGCTAACAACAATTTCTTTGATTTTCGTAACGGTGGATTGGCCATTCAGGGTGGGATTTTGTTAACCAGTATTGTCGGAGTAATCTATTTCAACTTCTTTTTAAATAGTAAGACCAATAAAACCAAAACGATTGCTAAATTACTGAATAATAAGAATGAAATAAAAGCTGTTTATGTTGAAAGAAATATCTCTGTTCTAGTGATGTTAGATCTGATTGCTCCTTGTGTTTTAATTGGTCAAGCAATTGGCAGATGGGGTAATTTTTTCAACCAAGAAGTTTATGGGTTTGCTTTAGCTGGAACAATGAATGATCCCCAAGCATTGGCTAATACCCAGTGGGGATTTTTAAAGATCTTAATGCCTAAGGTTTGGGATGGGATGTGGATTGATGGTCAGTTTCGCATTCCGCTCTTTTTAATTGAGTCATTTTTTAACACTATTTTCTTTGTGTTAATTTACTTTGTAATGGATTTTATTAGGGGAGTTAAAAGTGGCACAATTGGTTTTAGTTATTTTCTTGCTACTGGAATCATTCGTTTAATCTTGGAAAACTTTAGAGACCAAACCTTTTATTTTCCAACTTCAATAACCACTAGTATTTTGTTTATTGTCGTTGGTATTTTAGGAATTTTTTATTGCCAGTTTATCCATGTCAAATTAAGAAATTACTTCTGA", "aa": "MFVFKLIYCYKLQDNSFYFFIFIAVLTMVLGARLWSFVIGDSNFANNNFFDFRNGGLAIQGGILLTSIVGVIYFNFFLNSKTNKTKTIAKLLNNKNEIKAVYVERNISVLVMLDLIAPCVLIGQAIGRWGNFFNQEVYGFALAGTMNDPQALANTQWGFLKILMPKVWDGMWIDGQFRIPLFLIESFFNTIFFVLIYFVMDFIRGVKSGTIGFSYFLATGIIRLILENFRDQTFYFPTSITTSILFIVVGILGIFYCQFIHVKLRNYF", "aa_hexdigest": "7766424ce79c4bb2a8cc404aa9d0c663", "start_type": "ATG", "rbs_motif": null, "genes": [ "lgt" ], "truncated": "both", "pseudogene": { "start": 112687, "stop": 113835, "observations": { "mutation": [ 112702, 113593 ], "stop": [ 112702, 113593 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47332", "query_cov": 1.0, "subject_cov": 0.7015706806282722, "identity": 0.993, "score": 526.0, "evalue": 5.86e-187, "gene_start": 1, "gene_end": 268, "reference_start": 35, "reference_end": 302, "reference_sequence": "MNRPSWSTAFNIGGGFPIQWYGIIVSIGIIFAILMFVFKLIYCYKLQDNSFYFFIFIAVLTMVLGARLWSFVIGDSNFANNNFFDFRNGGLAIQGGILLTSIVGVIYFNFFLNSKTNKTKTIAELLNNKNEIKAVYVERNISVLVMLDLIAPCVLIGQAIGRWGNFFNQEVYGFALAGTMNDPQALANTQWGFLKILMPKVWDGMWIDGQFRIPLFLIESFFNTIFFVLIYFVMDFIRGVKSGTIGFSYFLATGIIRLILENFRDQTFYFQTSITTSILFIVVGILGIFYCQFIHVKLRNYFWTYFFLYAFYKVAAFFTTLFLNNRKQMAQQKFAFYEKSLPNKKRSFFEMKYYNDVTTPKIYRLTDQEMKLFDKLEAVTTS" }, "paralog": false, "identity": 0.9842931937172775, "score": 756.0, "evalue": 2.46e-280, "description": "Internal stop codon at 112702, 113593. Nonsense mutation around 112702, 113593.", "psc": { "uniref90_id": "UniRef90_P47332", "gene": "lgt", "product": "prolipoprotein diacylglyceryl transferase", "ec_ids": [ "2.5.1.145" ], "uniref50_id": "UniRef50_P47332", "cog_id": "COG0682", "cog_category": "M", "go_ids": [ "GO:0005886", "GO:0008961", "GO:0009249", "GO:0016020", "GO:0042158" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47332", "EC:2.5.1.145", "UniRef:UniRef50_P47332", "COG:COG0682", "COG:M", "GO:0005886", "GO:0008961", "GO:0009249", "GO:0016020", "GO:0042158" ] }, "pscc": { "uniref50_id": "UniRef50_P47332", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47332" ], "product": "Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase" } }, "id": "KMADJIBOEG_253", "locus": "GCA000292445_00214" }, { "type": "cds", "sequence": "contig_1", "start": 113671, "stop": 113835, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCACAACAGAAGTTTGCTTTTTATGAAAAATCACTTCCCAATAAGAAGCGTTCTTTTTTTGAAATGAAGTATTACAATGATGTAACAACACCCAAAATTTATCGTTTAACTGATCAGGAAATGAAGTTATTTGATAAATTAGAGCCAGTTACAACCAGCTAG", "aa": "MAQQKFAFYEKSLPNKKRSFFEMKYYNDVTTPKIYRLTDQEMKLFDKLEPVTTS", "aa_hexdigest": "c58057e73b93e4c21edb6b07c7778500", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6576.528700000001, "isoelectric_point": 9.301321220397949 }, "id": "KMADJIBOEG_254", "locus": "GCA000292445_00215" }, { "type": "cds", "sequence": "contig_1", "start": 113864, "stop": 113989, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTACAATAGCGCAATTAATTAGAAAACCACGCCAAAAAAAGAAGGTTAAATCAAAGTCACCTGCACTCCATTATAACCTCAACCTTTTAAACAAAAAACTACCAATGTTTACTCACCACTAA", "aa": "MATIAQLIRKPRQKKKVKSKSPALHYNLNLLNKKLPMFTHH", "aa_hexdigest": "7868583741dd99861bec2a850c876912", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4823.8217, "isoelectric_point": 11.362439918518067 }, "id": "KMADJIBOEG_255", "locus": "GCA000292445_00216" }, { "type": "cds", "sequence": "contig_1", "start": 114019, "stop": 114282, "strand": "+", "frame": 1, "gene": null, "product": "30S ribosomal protein S12", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A8J6YEE5" ], "nt": "ATGACCCCCAGAAAACCTAATTCTGCACTAAGAAAGTATGCTAAGGTCAGACTTACAAATGGTTTTGAAGTACTTGCTTATATCCCAGGAGAAGGTCATAACCTACAAGAACACAGTGTTACTTTATTAAGGGGGGGTAGAGTAAAAGATCTCCCTGGAGTTAGATACCATATTGTTCGTGGTACTTTAGATACAGTTGGTGTTGACAAAAGAAGACAACAACGTTCTGCATATGGCGCTAAAAAACCAAAACCAAAATCTTAA", "aa": "MTPRKPNSALRKYAKVRLTNGFEVLAYIPGEGHNLQEHSVTLLRGGRVKDLPGVRYHIVRGTLDTVGVDKRRQQRSAYGAKKPKPKS", "aa_hexdigest": "19c4b2ac811111f00d6cb5e7c84f0e72", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2P9HNB1", "query_cov": 0.9540229885057471, "subject_cov": 0.9540229885057471, "identity": 0.807, "score": 143.0, "evalue": 5.82e-43, "valid": false, "gene": "rpsL", "product": "30S ribosomal protein S12", "uniref50_id": "UniRef50_A0A8J6YEE5", "kegg_orthology_id": "K02950", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_A0A8J6YEE5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A8J6YEE5" ], "product": "30S ribosomal protein S12" }, "genes": [], "id": "KMADJIBOEG_256", "locus": "GCA000292445_00217" }, { "type": "cds", "sequence": "contig_1", "start": 114299, "stop": 114766, "strand": "+", "frame": 2, "gene": "rpsG", "product": "30S ribosomal protein S7", "db_xrefs": [ "COG:COG0049", "COG:J", "GO:0000028", "GO:0000049", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0022627", "KEGG:K02992", "RefSeq:WP_009885645.1", "SO:0001217", "UniParc:UPI0000135107", "UniRef:UniRef100_P47334", "UniRef:UniRef50_P21469", "UniRef:UniRef90_P47334" ], "nt": "ATGAGAAAAAATCGTGCTTTAAAAAGAACTGTTTTACCCGATCCTGTTTTTAACAACACACTGGTTACAAGGATTATTAATGTCATCATGAAAGATGGCAAGAAGGGTTTAGCACAACGCATCTTGTATGGTGCTTTTGAGATCATTGAAAAACGCACCAACCAACAACCTTTAACTGTCTTTGAAAAAGCAGTTGATAATGTTATGCCCCGCTTAGAGTTAAAAGTGAGAAGAATTGCTGGTTCTAACTACCAAGTACCAACTGAAGTTCCCCCTGACAGAAGGATTGCTTTAGCACTAAGATGGATTGTGATCTTTGCTAACAAAAGAAATGAAAAAACAATGCTTGAACGTGTTGCTAATGAAATTATTGATGCTTTTAATAACACGGGTGCTAGTGTTAAAAAGAAGGATGATACTCACAAGATGGCAGAAGCTAACAAAGCCTTTGCCCACATGCGTTGGTAG", "aa": "MRKNRALKRTVLPDPVFNNTLVTRIINVIMKDGKKGLAQRILYGAFEIIEKRTNQQPLTVFEKAVDNVMPRLELKVRRIAGSNYQVPTEVPPDRRIALALRWIVIFANKRNEKTMLERVANEIIDAFNNTGASVKKKDDTHKMAEANKAFAHMRW", "aa_hexdigest": "c83a70d858afbe8347c2153f5149a144", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000135107", "ncbi_nrp_id": "WP_009885645.1", "uniref100_id": "UniRef100_P47334", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000135107", "RefSeq:WP_009885645.1", "UniRef:UniRef100_P47334" ] }, "ips": { "uniref100_id": "UniRef100_P47334", "uniref90_id": "UniRef90_P47334" }, "psc": { "uniref90_id": "UniRef90_P47334", "gene": "rpsG", "product": "30S ribosomal protein S7", "uniref50_id": "UniRef50_P21469", "cog_id": "COG0049", "cog_category": "J", "kegg_orthology_id": "K02992", "go_ids": [ "GO:0000028", "GO:0000049", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P21469", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P21469" ], "product": "Small ribosomal subunit protein uS7" }, "genes": [ "rpsG" ], "id": "KMADJIBOEG_257", "locus": "GCA000292445_00218" }, { "type": "cds", "sequence": "contig_1", "start": 114776, "stop": 114991, "strand": "+", "frame": 2, "gene": "lepA", "product": "GTP-binding protein lepA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KNQ4" ], "nt": "ATGTCAAGAACAGTTGATTTAAAAAACTTCCGTAACTTTGGCATTATGGCCCATATTGATGCTGGGAAAACCACCACATCAGAACGTATTTTGTTCCATTCAGGTAGAATTCACAAGATTGGTGAAACCCATGATGGTGAATCAGTGATGGACTGGATGGAACAAGAAAAAGAAAGGGGTATTACTATCACCTCTGCAGCCACTTCAGTGAGCTGA", "aa": "MSRTVDLKNFRNFGIMAHIDAGKTTTSERILFHSGRIHKIGETHDGESVMDWMEQEKERGITITSAATSVS", "aa_hexdigest": "f943b1f57d508f2d22013d85c521b884", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3P8KNQ4", "query_cov": 0.971830985915493, "subject_cov": 0.9324324324324325, "identity": 0.7390000000000001, "score": 109.0, "evalue": 3.86e-30, "valid": false, "product": "GTP-binding protein lepA", "uniref50_id": "UniRef50_A0A3P8KNQ4" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KNQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KNQ4" ], "product": "GTP-binding protein lepA" }, "genes": [ "lepA" ], "id": "KMADJIBOEG_258", "locus": "GCA000292445_00219" }, { "type": "cds", "sequence": "contig_1", "start": 115010, "stop": 115135, "strand": "+", "frame": 2, "gene": null, "product": "GTP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000E2F5E1A", "UniRef:UniRef90_UPI000E2F5E1A" ], "nt": "TTGATTGACACTCCTGGCCATGTTGACTTTACAGTTGAAGTGGAGCGTAGCTTAAGGGTTTTGGATGGAGCAATTGCGGTATTGGATGCTCAAATGGGAGTAGAACCACAAACTGAAACAGTATGA", "aa": "MIDTPGHVDFTVEVERSLRVLDGAIAVLDAQMGVEPQTETV", "aa_hexdigest": "5fdc6e462382a49e41aa5e7c6538dacc", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 115004, "stop": 115222, "observations": { "mutation": [ 115133 ], "stop": [ 115133 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000E2F5E1A", "query_cov": 1.0, "subject_cov": 0.5256410256410257, "identity": 0.927, "score": 79.0, "evalue": 1.92e-18, "gene_start": 1, "gene_end": 41, "reference_start": 8, "reference_end": 48, "reference_sequence": "WRGHRVNIIDTPGHVDFTVEVERSLRVLDGAVAVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKIGADFLYSVG" }, "paralog": false, "identity": 0.8055555555555556, "score": 117.0, "evalue": 1.75e-36, "description": "Internal stop codon at 115133. Nonsense mutation around 115133.", "psc": { "uniref90_id": "UniRef90_UPI000E2F5E1A", "product": "GTP-binding protein", "uniref50_id": "UniRef50_UPI000E2F5E1A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000E2F5E1A", "UniRef:UniRef50_UPI000E2F5E1A" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000E2F5E1A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000E2F5E1A" ], "product": "GTP-binding protein" } }, "id": "KMADJIBOEG_259", "locus": "GCA000292445_00220" }, { "type": "cds", "sequence": "contig_1", "start": 115184, "stop": 115834, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAAACCGGTGCTAACTTTGAGCGCTCTGTTTTATCAATTCAACAACGCTTGGGAGTGAAAGCTGTTCCTATTCAATTTCCCATAGGTGCTGAAAATGATTTCAATGGCATCATTGATATCATCACTAAAAAAGCTTATTTTTTTGATGGTAATAAAGAGGAAAATGCTATTGAAAAACCAATTCCTGAACAGTATGTTGATCAAGTTGAAAAACTTTACAACAACTTAGTTGAAGAAGTTGCTAGTTTAGATGATCAACTCATGGCTGATTATCTAGATGGTAAACCAATTGAAATTGATGCAATTAAAAATGCAATTAGAAACGGGGTAATTCACTGTAAGTTTTTCCCGGTATTGTGTGGTTCAGCATTTAAAAACAAGGGAATTAAACTCTTACTTGATGCAGTGGTTGATTTTCTCCCTTCACCTGTTGATGTCCCACCTGCTAAAGCAATTGATGCAAACAACAAAGAGATATCTATTAAAGCTAGTGATGATGCTAACTTTATTGGCTTAGCATTTAAAGTTGCTACTGATCCTTTTGTTGGTAGATTAACTTTTATTAGGGTTTATGCAGGGGTTTTAAAATCTGGTTCTTATGTTAAGAATGTTAGAAAAACAAAAAGGAAAGGGTATCACGTTTAG", "aa": "MDKTGANFERSVLSIQQRLGVKAVPIQFPIGAENDFNGIIDIITKKAYFFDGNKEENAIEKPIPEQYVDQVEKLYNNLVEEVASLDDQLMADYLDGKPIEIDAIKNAIRNGVIHCKFFPVLCGSAFKNKGIKLLLDAVVDFLPSPVDVPPAKAIDANNKEISIKASDDANFIGLAFKVATDPFVGRLTFIRVYAGVLKSGSYVKNVRKTKRKGYHV", "aa_hexdigest": "6bd9394cbf12520b1e3e23718b38e009", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00316C015F", "query_cov": 0.8981481481481481, "subject_cov": 0.8622222222222222, "identity": 0.515, "score": 186.0, "evalue": 3.91e-56, "valid": false, "product": "GTP-binding protein", "uniref50_id": "UniRef50_A0A7S3AZE6" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23922.302100000008, "isoelectric_point": 7.6723203659057635 }, "id": "KMADJIBOEG_260", "locus": "GCA000292445_00221" }, { "type": "cds", "sequence": "contig_1", "start": 115840, "stop": 116841, "strand": "+", "frame": 1, "gene": "fusA", "product": "elongation factor G", "db_xrefs": [ "COG:COG0480", "COG:J", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006414", "GO:0032790", "KEGG:K02355", "SO:0001217", "UniRef:UniRef50_Q5SHN5", "UniRef:UniRef90_P75544" ], "nt": "ATGCACGCACAAAATCGCAATGAAATTGATGAAATTAGAGCAGGGGATATCTGTGCAGTAATTGGCTTGAAAGATACTACTACTGGAGAAACTTTAACTGATGATAAGCTTGATGTGCAACTAGAAGCAATGCAATTTGCTGAACCAGTGATCTCTTTAGCAGTAGAACCTAAAACTAAAGCAGATCAGGAAAAGATGTCAATTGCTTTATCAAAACTAGCAGAAGAAGATCCTACTTTTAAAACCTTTAGTGATCCTGAAACAGGGCAAACTATTATTGCTGGAATGGGTGAGTTACACCTTGATATCTTAGTTGATAGGATGAAACGTGAATTTAAGGTAGAAGTTAACATTGGTGCACCTCAAGTTAGCTTTCGTGAAACCTTTAAATCAACTAGTGAAGTTGAGGGTAAATACATCAAACAATCAGGTGGTAGAGGTCAATATGGACATGTTAAAATCCGTTTTGAACCTAATAAAGATAAGGGCTTTCAATTTGTTGATAAGATTGTGGGCGGAAGGATTCCAAGGGAATATATTAAACCAGTTCAAGCTGGTCTTGAAAATGCAATGAATTCAGGTCCTTTAGCAGGTTACCCAATGATTGATATTAAAGCTACCTTATTTGATGGTTCTTTCCATGAAGTTGACTCAAGTGAAATGGCTTTTAAAATTGCTGCATCCTTAGCTTTAAAAGAAGCAGGTAAACAATGTAACCCAGTTTTACTTGAACCTATTATGGCAATAGAAGTTACTGTACCTGAACAGTACTTTGGGGATACAATGGGTGATATCAGTTCAAGAAGAGGGATCATTGAAGGTACTGAACAACGTGATAATGTTCAACTAATAAAAGCAAAAGTACCTTTAAAAGAGATGTTTGGTTATGCCACTGATTTACGCTCTTTTTCCCAAGGTAGGGGTAATTATGTAATGCAATTTAGCCATTATGCTGAAACTCCTAAAAGCGTTGTTAATGAGATAATTGCTAATAAAAAATAG", "aa": "MHAQNRNEIDEIRAGDICAVIGLKDTTTGETLTDDKLDVQLEAMQFAEPVISLAVEPKTKADQEKMSIALSKLAEEDPTFKTFSDPETGQTIIAGMGELHLDILVDRMKREFKVEVNIGAPQVSFRETFKSTSEVEGKYIKQSGGRGQYGHVKIRFEPNKDKGFQFVDKIVGGRIPREYIKPVQAGLENAMNSGPLAGYPMIDIKATLFDGSFHEVDSSEMAFKIAASLALKEAGKQCNPVLLEPIMAIEVTVPEQYFGDTMGDISSRRGIIEGTEQRDNVQLIKAKVPLKEMFGYATDLRSFSQGRGNYVMQFSHYAETPKSVVNEIIANKK", "aa_hexdigest": "a86f74a7c958cc2f54203ddd54655324", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FE15F15", "query_cov": 0.987987987987988, "subject_cov": 0.9676470588235294, "identity": 0.7390000000000001, "score": 502.0, "evalue": 6.72e-177, "valid": false, "uniref50_id": "UniRef50_A0A2D5D5Z6" }, "genes": [ "fusA" ], "truncated": "5-prime", "pseudogene": { "start": 115541, "stop": 116841, "observations": { "deletion": [ 115805 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P75544", "query_cov": 1.0, "subject_cov": 0.48401162790697677, "identity": 0.9940000000000001, "score": 653.0, "evalue": 2.43e-231, "gene_start": 1, "gene_end": 333, "reference_start": 356, "reference_end": 688, "reference_sequence": "MSRTVDLKNFRNFGIMAHIDAGKTTTSERILFHSGRIHKIGETHDGESVMDWMEQEKERGITITSAATSVSWKNCSLNLIDTPGHVDFTVEVERSLRVLDGAIAVLDAQMGVEPQTETVWRQASRYEVPRVIFVNKMDKTGANFERSVLSIQQRLGVKAVPIQFPIGAENDFNGIIDIITKKAYFFDGNKEENAIEKPIPEQYVDQVEKLYNNLVEEVASLDDQLMADYLDGKPIEIDAIKNAIRNGVIHCKFFPVLCGSAFKNKGIKLLLDAVVDFLPSPVDVPPAKAIDANNKEISIKASDDANFIGLAFKVATDPFVGRLTFIRVYAGVLKSGSYVKNVRKNKKERVSRLVKMHAQNRNEIDEIRAGDICAVIGLKDTTTGETLTDDKLDVQLEAMQFAEPVISLAVEPKTKADQEKMSIALSKLAEEDPTFKTFSDPETGQTIIAGMGELHLDILVDRMKREFKVEVNIGAPQVSFRETFKSTSEVEGKYIKQSGGRGQYGHVKIRFEPNKDKGFEFVDKIVGGRIPREYIKPVQTGLENAMNSGPLAGYPMIDIKATLFDGSFHEVDSSEMAFKIAASLALKEAGKQCNPVLLEPIMAIEVTVPEQYFGDTMGDISSRRGIIEGTEQRDNVQLIKAKVPLKEMFGYATDLRSFSQGRGNYVMQFSHYAETPKSVVNEIIANKK" }, "paralog": false, "identity": 0.9953810623556582, "score": 841.0, "evalue": 1.22e-307, "description": "Frameshift due to deletion around 115805.", "psc": { "uniref90_id": "UniRef90_P75544", "gene": "fusA", "product": "elongation factor G", "uniref50_id": "UniRef50_Q5SHN5", "cog_id": "COG0480", "cog_category": "J", "kegg_orthology_id": "K02355", "go_ids": [ "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006414", "GO:0032790" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P75544", "UniRef:UniRef50_Q5SHN5", "COG:COG0480", "COG:J", "KEGG:K02355", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006414", "GO:0032790" ] }, "pscc": { "uniref50_id": "UniRef50_Q5SHN5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5SHN5" ], "product": "Elongation factor G" } }, "id": "KMADJIBOEG_261", "locus": "GCA000292445_00222" }, { "type": "cds", "sequence": "contig_1", "start": 116893, "stop": 117363, "strand": "+", "frame": 1, "gene": "rpsF", "product": "30S ribosomal protein S6", "db_xrefs": [ "COG:COG0360", "COG:J", "GO:0000314", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0022627", "GO:0070181", "GO:1990904", "KEGG:K02990", "SO:0001217", "UniRef:UniRef50_P47336", "UniRef:UniRef90_P47336" ], "nt": "ATGCACTACAATATCATTCTTTTAGTTGATGGTACGCTTAGTTTAGAACAAGCTAACCAAGTTGAACAAAAACACCAAAAATTGCTTGAAAAGGCAACTGAATTTAAAAGTGAATACTTAGGTTTAAAAGAGTTGGCTTACCCCATTAAAAAGCAACTTTCTGCTCACTATTACAGATGGAGTTTTCATGGTGAAAGCAATTGTACTAAGGAGTTTAAAAGAGCTGCTAACATCAATAAGCAGATAATAAGAGAGTTAATTATTAACAGAGAAAAAGACTATGGTTATTTAGGTTCAGTTAACCCTAAAAAACAACAACTGTCTTTGCAGAAGCTAACCAAGTATAATGAGATTATTGCTAGTGAAAATAATCCTGATAACCCAGATGCGCCTGTCACTTCTGGTCTAGCTTCTGTTAAACCACGGCTATCAAGAGTTGAAAAACAAAAGGAACGTGAACTTGAAAAGTGA", "aa": "MHYNIILLVDGTLSLEQANQVEQKHQKLLEKATEFKSEYLGLKELAYPIKKQLSAHYYRWSFHGESNCTKEFKRAANINKQIIRELIINREKDYGYLGSVNPKKQQLSLQKLTKYNEIIASENNPDNPDAPVTSGLASVKPRLSRVEKQKERELEK", "aa_hexdigest": "97462fe86103323364dc6241cad6a575", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpsF" ], "truncated": "3-prime", "pseudogene": { "start": 116893, "stop": 117519, "observations": { "mutation": [ 117361 ], "stop": [ 117361 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47336", "query_cov": 1.0, "subject_cov": 0.75, "identity": 1.0, "score": 301.0, "evalue": 8.18e-103, "gene_start": 1, "gene_end": 156, "reference_start": 1, "reference_end": 156, "reference_sequence": "MHYNIILLVDGTLSLEQANQVEQKHQKLLEKATEFKSEYLGLKELAYPIKKQLSAHYYRWSFHGESNCTKEFKRAANINKQIIRELIINREKDYGYLGSVNPKKQQLSLQKLTKYNEIIASENNPDNPDAPVTSGLASVKPRLSRVEKQKERELEKWTVVHQSGNFDTVQINPYRPRIKRFLQNNQQTSQANNNQPRFQNQFKKGAKP" }, "paralog": false, "identity": 0.9951923076923077, "score": 416.0, "evalue": 2.09e-150, "description": "Internal stop codon at 117361. Nonsense mutation around 117361.", "psc": { "uniref90_id": "UniRef90_P47336", "gene": "rpsF", "product": "30S ribosomal protein S6", "uniref50_id": "UniRef50_P47336", "cog_id": "COG0360", "cog_category": "J", "kegg_orthology_id": "K02990", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0022627", "GO:0070181", "GO:1990904" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47336", "UniRef:UniRef50_P47336", "COG:COG0360", "COG:J", "KEGG:K02990", "GO:0000314", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0022627", "GO:0070181", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P47336", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47336" ], "product": "Small ribosomal subunit protein bS6" } }, "id": "KMADJIBOEG_262", "locus": "GCA000292445_00223" }, { "type": "cds", "sequence": "contig_1", "start": 117712, "stop": 118002, "strand": "+", "frame": 1, "gene": "ssb", "product": "Single-stranded DNA-binding protein", "db_xrefs": [ "COG:COG0629", "COG:L", "GO:0003697", "GO:0006260", "GO:0009295", "SO:0001217", "UniRef:UniRef50_P75542", "UniRef:UniRef90_P47337" ], "nt": "ATGCTTTTTGTAGAAGGAAGATTAACTACATATAAAATTACTAACAGTGAAAATAAAAACACCTATGCTTTACAAGTAACTGCTGATAAGATCTTTCATCCTGATGAAAAAACTACCAATGAAGAACCTATTAAATCAACTGTAGTTGATTCACCCTTTATGAATCCCAAAGCAAGTGTTACAGAAGCTGAGTTTGAACAAGCATTCCCCCATCAAGATGAAACTGATTTTAACAACATTACCCCTATATTTGAAAATGATGTCCAACTAGAGGAGGAAAGTGATGATTAA", "aa": "MLFVEGRLTTYKITNSENKNTYALQVTADKIFHPDEKTTNEEPIKSTVVDSPFMNPKASVTEAEFEQAFPHQDETDFNNITPIFENDVQLEEESDD", "aa_hexdigest": "63c4e090fab5027f158933171c84a084", "start_type": "ATG", "rbs_motif": null, "genes": [ "ssb" ], "truncated": "5-prime", "pseudogene": { "start": 117520, "stop": 118002, "observations": { "mutation": [ 117667 ], "stop": [ 117667 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47337", "query_cov": 1.0, "subject_cov": 0.6, "identity": 1.0, "score": 190.0, "evalue": 2.11e-60, "gene_start": 1, "gene_end": 96, "reference_start": 65, "reference_end": 160, "reference_sequence": "MNRVFLFGKLSFTPNRLQTKNGTLGATFSMECLDSSGFNNAKSFIRVTAWGKVASFIVAQNPGVMLFVEGRLTTYKITNSENKNTYALQVTADKIFHPDEKTTNEEPIKSTVVDSPFMNPKASVTEAEFEQAFPHQDETDFNNITPIFENDVQLEEESDD" }, "paralog": false, "identity": 0.99375, "score": 319.0, "evalue": 8.89e-114, "description": "Internal stop codon at 117667. Nonsense mutation around 117667.", "psc": { "uniref90_id": "UniRef90_P47337", "gene": "ssb", "product": "Single-stranded DNA-binding protein", "uniref50_id": "UniRef50_P75542", "cog_id": "COG0629", "cog_category": "L", "go_ids": [ "GO:0009295", "GO:0003697", "GO:0006260" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47337", "UniRef:UniRef50_P75542", "COG:COG0629", "COG:L", "GO:0009295", "GO:0003697", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_P75542", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75542" ], "product": "Single-stranded DNA-binding protein" } }, "id": "KMADJIBOEG_263", "locus": "GCA000292445_00224" }, { "type": "cds", "sequence": "contig_1", "start": 117995, "stop": 118309, "strand": "+", "frame": 2, "gene": "rpsR", "product": "30S ribosomal protein S18", "db_xrefs": [ "COG:COG0238", "COG:J", "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "GO:0070181", "RefSeq:WP_009885648.1", "SO:0001217", "UniParc:UPI0000557C7D", "UniRef:UniRef100_P47338", "UniRef:UniRef50_P47338", "UniRef:UniRef90_P47338" ], "nt": "ATGATTAATAAAGAACAGGATTTAAACCAATTAGAAACCAACCAAGAACAGAGTGTTGAACAAAACCAAACTGATGAAAAGCGCAAGCCAAAACCAAACTTTAAAAGAGCAAAAAAATATTGTCGATTTTGCGCCATAGGTCAACTAAGGATTGATTTTATTGATGATTTGGAAGCAATCAAACGCTTTCTCAGTCCCTATGCAAAGATTAATCCTAGAAGAATTACAGGTAATTGCAACATGCACCAACGTCATGTAGCTAATGCTCTAAAACGAGCACGTTACCTAGCTTTAGTGCCATTTATTAAAGATTAA", "aa": "MINKEQDLNQLETNQEQSVEQNQTDEKRKPKPNFKRAKKYCRFCAIGQLRIDFIDDLEAIKRFLSPYAKINPRRITGNCNMHQRHVANALKRARYLALVPFIKD", "aa_hexdigest": "f3db5ce4b19d0a295cf2c340b55e09b3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000557C7D", "ncbi_nrp_id": "WP_009885648.1", "uniref100_id": "UniRef100_P47338", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000557C7D", "RefSeq:WP_009885648.1", "UniRef:UniRef100_P47338" ] }, "ips": { "uniref100_id": "UniRef100_P47338", "uniref90_id": "UniRef90_P47338" }, "psc": { "uniref90_id": "UniRef90_P47338", "gene": "rpsR", "product": "30S ribosomal protein S18", "uniref50_id": "UniRef50_P47338", "cog_id": "COG0238", "cog_category": "J", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "GO:0070181" ] }, "pscc": { "uniref50_id": "UniRef50_P47338", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47338" ], "product": "Small ribosomal subunit protein bS18" }, "genes": [ "rpsR" ], "id": "KMADJIBOEG_264", "locus": "GCA000292445_00225" }, { "type": "cds", "sequence": "contig_1", "start": 118312, "stop": 118764, "strand": "+", "frame": 1, "gene": "rplI", "product": "50S ribosomal protein L9", "db_xrefs": [ "COG:COG0359", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "KEGG:K02939", "RefSeq:WP_009885649.1", "SO:0001217", "UniParc:UPI00001341F1", "UniRef:UniRef100_P47339", "UniRef:UniRef50_P75540", "UniRef:UniRef90_P47339" ], "nt": "ATGAAGATAATTTTGAAGCAAGATGTTGCTAAATTAGGCAAGCGGTTTGATGTTGTTGAAGTTAAAGATGGGTTTGCTATCCATTTTTTATTTCCCAAAAAACTAGCTGCACCTTTAACAAAGAAAGCAATTGCTAACCGTGATTTGTTTTTAAAACAACAACAAGAACAATACCAAAAAAATCGTGCCTTAGCTGAAAAATTGAAACTAGTAATTGAACAAACACCATTAACTTTTCAACTCAAACAACACGATGGCAAGCCATATGGTTCAATCATCACCAAACAAATAATTAATTTAGCAAAACAACAAAGACTTGATTTACAGCGCTTTATGTTTAAAGATAATGTGCGCTTACAGTTTGGTGAACACAAACTAATTTTGCACCTTTTTGAGGAGATAACTGCAACTTTAACTGTTATAGTGAACCCTGAAAATGGGACAACAAACTAG", "aa": "MKIILKQDVAKLGKRFDVVEVKDGFAIHFLFPKKLAAPLTKKAIANRDLFLKQQQEQYQKNRALAEKLKLVIEQTPLTFQLKQHDGKPYGSIITKQIINLAKQQRLDLQRFMFKDNVRLQFGEHKLILHLFEEITATLTVIVNPENGTTN", "aa_hexdigest": "bcfbc0281b4519f95d168d7380d55c03", "start_type": "ATG", "rbs_motif": "ATTTA", "ups": { "uniparc_id": "UPI00001341F1", "ncbi_nrp_id": "WP_009885649.1", "uniref100_id": "UniRef100_P47339", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001341F1", "RefSeq:WP_009885649.1", "UniRef:UniRef100_P47339" ] }, "ips": { "uniref100_id": "UniRef100_P47339", "uniref90_id": "UniRef90_P47339" }, "psc": { "uniref90_id": "UniRef90_P47339", "gene": "rplI", "product": "50S ribosomal protein L9", "uniref50_id": "UniRef50_P75540", "cog_id": "COG0359", "cog_category": "J", "kegg_orthology_id": "K02939", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P75540", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75540" ], "product": "Large ribosomal subunit protein bL9" }, "genes": [ "rplI" ], "id": "KMADJIBOEG_265", "locus": "GCA000292445_00226" }, { "type": "cds", "sequence": "contig_1", "start": 118748, "stop": 119377, "strand": "+", "frame": 2, "gene": "dnaB", "product": "Replicative DNA helicase DnaB", "db_xrefs": [ "COG:COG0305", "COG:L", "EC:5.6.2.3", "GO:0003677", "GO:0003678", "GO:0003689", "GO:0005524", "GO:0005829", "GO:0006268", "GO:0006269", "GO:0009378", "GO:0016853", "GO:0016887", "GO:0036121", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990077", "GO:1990518", "KEGG:K02314", "SO:0001217", "UniRef:UniRef50_P47340", "UniRef:UniRef90_P47340" ], "nt": "ATGGGACAACAAACTAGTTTTAAATATGCAAATGATAGCAACATTGAACGTGCTGAAAGACGTTTGATGCAAGCAGTTGCTCAAAACAGTGAGGGCATTGATCTAATTTTCAATAAACTTGAACCAATTGATTTTTTTGCAACCCCTTTCAAACTCATTTTTCAAACTGCAAAAGAAAACTACCAATTAAATAACCCTATTATTGGTTCTGGTTTACTAGAAGCGGTTAAGTTTAAACTTGATGCTAATGATCAATCCACTAAAAGTGAACTTGAAATTTTATTCACAAAGATCTTATTAATCCGTTTACCACCTAACCAAACAGAGATTAAAACACTGGTTGATGTTGTTAAAAAAGCTTCTATTTTTCGCAGGTTACAACAGTTTGCTAAGCGTGTTTACAACGAGGAATTTAAGTTAAAAGAAGATCGTTTTGAAGGCTATTTACAAGCTATTCAAGATGATTTTGTCAAGATTATCCACAGTGCTTTTAGTAACATCTTTGCTTTTAGCTATGATGAGATTGCCAATCAAGAGGAAGCATTAATTAAAAAGGTTCACCGTGGGGAATTGATCATCAGTGGACTTTCAAGTGGATTTTTAAAATTAGATCAACTTACATCAGGTTGA", "aa": "MGQQTSFKYANDSNIERAERRLMQAVAQNSEGIDLIFNKLEPIDFFATPFKLIFQTAKENYQLNNPIIGSGLLEAVKFKLDANDQSTKSELEILFTKILLIRLPPNQTEIKTLVDVVKKASIFRRLQQFAKRVYNEEFKLKEDRFEGYLQAIQDDFVKIIHSAFSNIFAFSYDEIANQEEALIKKVHRGELIISGLSSGFLKLDQLTSG", "aa_hexdigest": "9fc704a78e823d94adaf9bdad0485551", "start_type": "ATG", "rbs_motif": "TTATA", "genes": [ "dnaB" ], "truncated": "3-prime", "pseudogene": { "start": 118748, "stop": 119680, "observations": { "mutation": [ 119375 ], "stop": [ 119375 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47340", "query_cov": 1.0, "subject_cov": 0.4465811965811966, "identity": 1.0, "score": 400.0, "evalue": 5.36e-137, "gene_start": 1, "gene_end": 209, "reference_start": 1, "reference_end": 209, "reference_sequence": "MGQQTSFKYANDSNIERAERRLMQAVAQNSEGIDLIFNKLEPIDFFATPFKLIFQTAKENYQLNNPIIGSGLLEAVKFKLDANDQSTKSELEILFTKILLIRLPPNQTEIKTLVDVVKKASIFRRLQQFAKRVYNEEFKLKEDRFEGYLQAIQDDFVKIIHSAFSNIFAFSYDEIANQEEALIKKVHRGELIISGLSSGFLKLDQLTSGWKPGELIVIAARPGRGKTALLINFMASAAKQIDPKTDVVLFFSLEMRNREIYQRHLMHESQTSYTLTNRQRINNVFEELMEASSRIKNLPIKLFDYSSLTLQEIRNQITEVSKTSNVRLVIIDYLQLVNALKNNYGLTRQQEVTMISQSLKAFAKEFNTPIIAAAQLSRRIEERKDSRPILSDLRESGSIEQDADMVLFIHRTNDDKKEQEEENTNLFEVELILEKNRNGPNGKVKLNFRSDTSSFISQYSPSFDDQYS" }, "paralog": false, "identity": 0.9967741935483871, "score": 595.0, "evalue": 3.51e-216, "description": "Internal stop codon at 119375. Nonsense mutation around 119375.", "psc": { "uniref90_id": "UniRef90_P47340", "gene": "dnaB", "product": "Replicative DNA helicase DnaB", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_P47340", "cog_id": "COG0305", "cog_category": "L", "kegg_orthology_id": "K02314", "go_ids": [ "GO:0005829", "GO:1990077", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0003678", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0006269", "GO:0006268" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47340", "EC:5.6.2.3", "UniRef:UniRef50_P47340", "COG:COG0305", "COG:L", "KEGG:K02314", "GO:0005829", "GO:1990077", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0003678", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0006269", "GO:0006268" ] }, "pscc": { "uniref50_id": "UniRef50_P47340", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47340" ], "product": "Replicative DNA helicase DnaB" } }, "id": "KMADJIBOEG_266", "locus": "GCA000292445_00227" }, { "type": "cds", "sequence": "contig_1", "start": 119447, "stop": 120154, "strand": "+", "frame": 2, "gene": "dnaB", "product": "Replicative DNA helicase DnaB", "db_xrefs": [ "COG:COG0305", "COG:L", "EC:5.6.2.3", "GO:0003677", "GO:0003678", "GO:0003689", "GO:0005524", "GO:0005829", "GO:0006268", "GO:0006269", "GO:0009378", "GO:0016853", "GO:0016887", "GO:0036121", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990077", "GO:1990518", "KEGG:K02314", "SO:0001217", "UniRef:UniRef50_P47340", "UniRef:UniRef90_P47340" ], "nt": "ATGGCTAGTGCAGCTAAACAAATTGATCCTAAAACTGATGTGGTCCTCTTCTTTAGTTTAGAGATGCGTAACCGGGAAATTTACCAAAGGCACTTAATGCATGAAAGTCAAACTAGTTACACACTAACCAACCGGCAAAGGATTAATAATGTCTTTGAAGAGTTAATGGAAGCATCTTCAAGGATCAAAAACTTACCTATTAAACTCTTTGATTACAGTAGTTTAACACTCCAAGAGATCAGAAACCAAATTACTGAAGTGAGTAAAACCAGTAATGTTAGGTTAGTAATTATTGACTATTTACAACTTGTTAATGCTTTAAAAAATAACTATGGTTTGACAAGACAACAAGAAGTGACAATGATCTCTCAATCACTTAAAGCATTCGCTAAGGAGTTTAATACCCCTATTATTGCTGCAGCTCAACTTTCTAGAAGGATTGAAGAAAGGAAAGATTCCAGACCAATTCTTTCTGATTTAAGAGAATCAGGTTCAATTGAACAGGATGCGGATATGGTTTTATTTATCCATAGAACTAATGATGATAAAAAAGAACAGGAAGAGGAGAACACAAACTTGTTTGAAGTGGAGCTTATCTTAGAAAAGAACAGAAATGGTCCCAATGGCAAAGTTAAACTAAACTTTCGCAGTGACACTTCTTCTTTTATTAGTCAATATTCCCCTAGTTTTGATGACCAATACAGTTAA", "aa": "MASAAKQIDPKTDVVLFFSLEMRNREIYQRHLMHESQTSYTLTNRQRINNVFEELMEASSRIKNLPIKLFDYSSLTLQEIRNQITEVSKTSNVRLVIIDYLQLVNALKNNYGLTRQQEVTMISQSLKAFAKEFNTPIIAAAQLSRRIEERKDSRPILSDLRESGSIEQDADMVLFIHRTNDDKKEQEEENTNLFEVELILEKNRNGPNGKVKLNFRSDTSSFISQYSPSFDDQYS", "aa_hexdigest": "b5651c07e719f73098f7488ccd5f6632", "start_type": "ATG", "rbs_motif": null, "genes": [ "dnaB" ], "truncated": "5-prime", "pseudogene": { "start": 119147, "stop": 120154, "observations": { "mutation": [ 119375 ], "stop": [ 119375 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47340", "query_cov": 1.0, "subject_cov": 0.5021367521367521, "identity": 1.0, "score": 441.0, "evalue": 8.85e-153, "gene_start": 1, "gene_end": 235, "reference_start": 234, "reference_end": 468, "reference_sequence": "MGQQTSFKYANDSNIERAERRLMQAVAQNSEGIDLIFNKLEPIDFFATPFKLIFQTAKENYQLNNPIIGSGLLEAVKFKLDANDQSTKSELEILFTKILLIRLPPNQTEIKTLVDVVKKASIFRRLQQFAKRVYNEEFKLKEDRFEGYLQAIQDDFVKIIHSAFSNIFAFSYDEIANQEEALIKKVHRGELIISGLSSGFLKLDQLTSGWKPGELIVIAARPGRGKTALLINFMASAAKQIDPKTDVVLFFSLEMRNREIYQRHLMHESQTSYTLTNRQRINNVFEELMEASSRIKNLPIKLFDYSSLTLQEIRNQITEVSKTSNVRLVIIDYLQLVNALKNNYGLTRQQEVTMISQSLKAFAKEFNTPIIAAAQLSRRIEERKDSRPILSDLRESGSIEQDADMVLFIHRTNDDKKEQEEENTNLFEVELILEKNRNGPNGKVKLNFRSDTSSFISQYSPSFDDQYS" }, "paralog": false, "identity": 0.9970149253731343, "score": 644.0, "evalue": 4.69e-235, "description": "Internal stop codon at 119375. Nonsense mutation around 119375.", "psc": { "uniref90_id": "UniRef90_P47340", "gene": "dnaB", "product": "Replicative DNA helicase DnaB", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_P47340", "cog_id": "COG0305", "cog_category": "L", "kegg_orthology_id": "K02314", "go_ids": [ "GO:0005829", "GO:1990077", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0003678", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0006269", "GO:0006268" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47340", "EC:5.6.2.3", "UniRef:UniRef50_P47340", "COG:COG0305", "COG:L", "KEGG:K02314", "GO:0005829", "GO:1990077", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0003678", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0006269", "GO:0006268" ] }, "pscc": { "uniref50_id": "UniRef50_P47340", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47340" ], "product": "Replicative DNA helicase DnaB" } }, "id": "KMADJIBOEG_267", "locus": "GCA000292445_00228" }, { "type": "cds", "sequence": "contig_1", "start": 120158, "stop": 120505, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACTGAAGTTTTACAAACTACCTTTAATTACTACAGCATTTAGTTTTGTATTTTTAACTGCTTGTTCAACACCCCAATCTTCCTTTTTTCTCCCTGCACAAACAACTATTAGTACTTTAAAAATTAATGGTATGGAAAACAAAACAGGTTATTTTCTTGAAACGCAGCGTTCTCGTGGTAGTTACAATCCCACCGCTTCACTTACTATGATTAAGTTAGGAGATGAAAAAGAGTTTAAAAACGTAGATACAACCAAGCAAGATGAAGTTTTGTTTGCCAACATTTATGGTGGAATCTCTTCATTGCTTAACTTCAGAATTATCCAACCGATGTTAACCTACTGA", "aa": "MKLKFYKLPLITTAFSFVFLTACSTPQSSFFLPAQTTISTLKINGMENKTGYFLETQRSRGSYNPTASLTMIKLGDEKEFKNVDTTKQDEVLFANIYGGISSLLNFRIIQPMLTY", "aa_hexdigest": "4239874428945bbd1f667f1e9f076b0c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13014.951299999982, "isoelectric_point": 9.300869941711426 }, "id": "KMADJIBOEG_268", "locus": "GCA000292445_00229" }, { "type": "cds", "sequence": "contig_1", "start": 120824, "stop": 120952, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAGGTATTGAAAATATTGAAGTTGAGTTTAGCTTTGTTAAAACTGGTTTTAATGGAAATGAGATTAAGTTTGAAGATTATGTAACAGATTCTTCCCCCACAACATCTTTATTAAAGCAAGTTTGA", "aa": "MIGIENIEVEFSFVKTGFNGNEIKFEDYVTDSSPTTSLLKQV", "aa_hexdigest": "8ed62026fe40d4ea6cd97c5ff932fbec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4728.241900000001, "isoelectric_point": 4.250954246520997 }, "id": "KMADJIBOEG_269", "locus": "GCA000292445_00230" }, { "type": "cds", "sequence": "contig_1", "start": 121181, "stop": 121354, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTACTAACTGATTTGCAAAAGAAACAAGATCAAGTGAATCGGTTTGTTGGTTTTATTGGTCAAAACCACTTTACTTTAAGTGCTAACAGCATTAATGAAAAGCAGTTTAACGATGCTAGTACTGCTGATTTTTTTAAAGCAATCTTTCAAAAAGTGAGTATCAATGAATAA", "aa": "MLLTDLQKKQDQVNRFVGFIGQNHFTLSANSINEKQFNDASTADFFKAIFQKVSINE", "aa_hexdigest": "b143ce62319daa644f13bee0d843ce30", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6538.271699999999, "isoelectric_point": 6.541849708557129 }, "id": "KMADJIBOEG_270", "locus": "GCA000292445_00231" }, { "type": "cds", "sequence": "contig_1", "start": 121538, "stop": 123169, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MG096", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47342", "UniRef:UniRef90_P47342" ], "nt": "TTGAAAACACAAAAAAATATCTTTAAGGTCAAGGCCTTACTACTTTCACTTTTTTCTGCTGCTGTTACCATCACTATTTTTGCTTTACCTATCTTTGCAAACAATGGTTCTAAAACTGATTTAGGTTTGCTTTCTAAAAATAGTGCTGATTTTTTAGGTAGTTCAAAAAGAAGTCTTGCTGGCTTTGATACTCCTTTTAGTCCAGATAACCTCCAGTATTTAGAAAAAGAAACTGACTATGATCAGAACTTTAAAAATTTTACTGAAAAGTTTAAAGATGAAAAAATAACTAACAATCAACTTGATATTGTTGATATCTATAACCTATTCAGTGGTTTTCACAAGAGTGTCAAAAACACAGTTGACTTAATGAGCCAACTGCAAAAGCAAGTTGAAGCTGCCAATGCTATCTTCCCAGTTGATGATGCTTTTGTTAAATTACCTAAAGTACCTACTGAATTATTTAAGCTAGTTGATGATAATGTCTTTCCTAAGTTAAATCCTAAGGGCTTAAATATCTCTGATAATATTGCTGCACTTTTTGAAAGATATAACCTTAAATCGATTGAACTTAAAAACTTTGATTTAGCTTTCTTGAGAAAAGCTGATGTAATTATCAAAGATAAGGTTCGATACAACTTTGAGATGCAAATGCAGTTTCAAACTGTTTATGTTGGTGGAGGGAATACCGTTATTAACTTAGACTTTACTTTAAAAGCCCAAACCGTTAACTTTGCTAACCTCCAAGATTTACAAAACACTTTTGTTAAAGTTGGTAATGATCTTTCCACCCAACTCTTTTGGATTCCAACTGTTAATAAATTAACTGATAATGCAGGTAATGATCTTACCCATATTGCTAAAACTGTGATTGGTGAATCGTTTTTCCAAACCAATGTTAACTTAGCTAAATCAGTTATTGAATATGATAAGGTTCAACCATTGGTTAAACAAGCTTTTGAAGAGCGAGTTTTAACTCCTTTCAAAAAGGAAAGAGAAGCTGCTAAAAAAGCTTATGAAGAAGAACAACGTCGCTTGGAAGAGGAACGTAAGCGTCAACTAGAAGAGCTAAGGAGAAGAGAAGCTGAGGAGAAAAGAAAAGCTGAAGAAGCAAAACGAAATCAAGAAAAAGCACGCAGAGAAAGAGAAGCTTATGAAAAATCATTTAACTCCTTTAACTCCTTTAAATTTTACTGGTTAACTAAAGGTAAAGATGTTACTAAAAAAGCTGATTTAATTGATGCACTTAAAACCGCTATTGCTACACCAGCATACAGAAATAGAACATTCTCTTTATTAATCAAAGGTTTTGCTAGTGGAGTTGAACGTTATTTCAACGCTAACAAGAATGATAAAGAGTTGAAAAAACTTGCATTTGGTGAAAAAGGGATCCAATTCCCTAGAGCAGATGCTGGGATAGATGGTCTTTACATGAGTAATTTTTTGAGGCATGAATTGACAAGCAAAGCAAAATTTTCACTTAACCTAAAAGATATAAAAGTTGAAAATACGGTTGAAGATACTCAATTGTATTGGAAGGATAATGGCATTCATTTAAAGCAAGCCAATCCTTATAAATTCAATCTAAATATCAAGATCAAGTACAACGGCTGGTATAATGTCCACTGGTGA", "aa": "MKTQKNIFKVKALLLSLFSAAVTITIFALPIFANNGSKTDLGLLSKNSADFLGSSKRSLAGFDTPFSPDNLQYLEKETDYDQNFKNFTEKFKDEKITNNQLDIVDIYNLFSGFHKSVKNTVDLMSQLQKQVEAANAIFPVDDAFVKLPKVPTELFKLVDDNVFPKLNPKGLNISDNIAALFERYNLKSIELKNFDLAFLRKADVIIKDKVRYNFEMQMQFQTVYVGGGNTVINLDFTLKAQTVNFANLQDLQNTFVKVGNDLSTQLFWIPTVNKLTDNAGNDLTHIAKTVIGESFFQTNVNLAKSVIEYDKVQPLVKQAFEERVLTPFKKEREAAKKAYEEEQRRLEEERKRQLEELRRREAEEKRKAEEAKRNQEKARREREAYEKSFNSFNSFKFYWLTKGKDVTKKADLIDALKTAIATPAYRNRTFSLLIKGFASGVERYFNANKNDKELKKLAFGEKGIQFPRADAGIDGLYMSNFLRHELTSKAKFSLNLKDIKVENTVEDTQLYWKDNGIHLKQANPYKFNLNIKIKYNGWYNVHW", "aa_hexdigest": "77a4449a916567705f67d1ad8bf84882", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47342", "query_cov": 0.998158379373849, "subject_cov": 0.8325652841781874, "identity": 0.98, "score": 946.0, "evalue": 0.0, "valid": true, "product": "Uncharacterized protein MG096", "uniref50_id": "UniRef50_P47342" }, "pscc": { "uniref50_id": "UniRef50_P47342", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47342" ], "product": "Uncharacterized protein MG096" }, "genes": [], "id": "KMADJIBOEG_271", "locus": "GCA000292445_00232" }, { "type": "cds", "sequence": "contig_1", "start": 123221, "stop": 123490, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "ATGAATTTAACATTTGTTGTCAACGGTGACCTATCAGAAATTGTTGATAAACATGATTACCCTGGTACTTTCTTTCAATTTACCGATAAAAATGAATTGCTATTTACCTTAGCAGTTAGAGAACAAATTAAGGTTGATAATAATCATTTTATGGGTCTGTTAAAAAGCCAAAACCTTCATAATCTTCAGCTTGCTTCTGGTGCTACAAAACCTCCTGTTGTTGATTTAGCTAGTTATTTCCACTTTGTATTATTGACTGAAAAATCTTAA", "aa": "MNLTFVVNGDLSEIVDKHDYPGTFFQFTDKNELLFTLAVREQIKVDNNHFMGLLKSQNLHNLQLASGATKPPVVDLASYFHFVLLTEKS", "aa_hexdigest": "faa8681328b3bb4030f9cf2cf21a4b41", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 47, "aa_cov": 0.5168539325842697, "hmm_cov": 1.0, "evalue": 3.4604935911401685e-13, "score": 44.97151565551758, "start": 1, "stop": 46 } ], "seq_stats": { "molecular_weight": 10112.420499999991, "isoelectric_point": 5.675168800354004 }, "id": "KMADJIBOEG_272", "locus": "GCA000292445_00233" }, { "type": "cds", "sequence": "contig_1", "start": 124018, "stop": 124245, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTAACTTTTCTAAAAAATCAAAACATTAAACCGCTGTTGGTATTTCTGGGTGTTCAAACTAAAAACTTTGTTGTTAAGAGTATTGGTAATGTTGATGGATTTGAGCATTTATCATATCCCCATCCCTCACCACTAAGTGGTAATTTGTTTCTAACAAACCCTAACGATCTGTTTAAAACAATTAACAATTGGTTGAAACAACATAACCAAAAAATAATTAACTGA", "aa": "MLTFLKNQNIKPLLVFLGVQTKNFVVKSIGNVDGFEHLSYPHPSPLSGNLFLTNPNDLFKTINNWLKQHNQKIIN", "aa_hexdigest": "39ebb89078017ab907b4aa6e583be638", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8589.877999999997, "isoelectric_point": 9.778645133972166 }, "id": "KMADJIBOEG_273", "locus": "GCA000292445_00234" }, { "type": "cds", "sequence": "contig_1", "start": 124384, "stop": 125121, "strand": "+", "frame": 1, "gene": "gatC", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC", "db_xrefs": [ "COG:COG0721", "COG:J", "GO:0005886", "GO:0006424", "GO:0006450", "GO:0030956", "GO:0050567", "GO:0070681", "SO:0001217", "UniRef:UniRef50_P47344", "UniRef:UniRef90_P47344" ], "nt": "ATGGGAGAGATTAAAAATACTGCACCAACAAGCGATATTTCCACTTCAGGATTTATCTATTTTGCAGTTGTTTTTCTAATCATTATAGTCTATCTTTTTTTTAAAAACATTCTCTTTTTGTTCTTCTTTAAAAGATATCCTAAAAACACACCTAAAATTGGGGTTAGCAATATTACTACTATTGCTATGATTATTGCTGTTGCTGTATCTGTTGTTTTGGTCTTAATGGCTTTAGCAGGGGGGTTAACAGCAGCGCTGTTCCGTGGTTATCCTGGGTTCCGTGTTACCTTAGAGTTAATTCTAGTGAAGATTTCAGGACTTTTATTTGGTCCTATAATTGGTATTTTTTCAGCAGCTACCATTGACTTTTTAACTGTTATTTTTTCAGGTGGGGTGTTTAATATTGGTTATGTTTTAGGAGCAATCCTAACGGGAATGATTGCTGGAATTTTACGTGAAGTTTTAATTTCAACTTCTTTTTTAAATAACAAAACTTTAAGTGATTTTGCCTATCTTGTTTTATCAGTGGGGATGGTATTTGCCAGTTTTTTAGTAACCCAGTTTTTTGTTATCTCAGTTACCCAAAACTTATCAGCATTTCAAAGTAATGATCAAATTGTTTTACGTTTTAACGCCTCACCTTTAAATTTCAGTATCTCATTGCAAAGATATGTTCAAATTATTTTCTATTTTGCGATGGTTGTAATCATCACAATGGTAGTACTTTACTTTGTTTGA", "aa": "MGEIKNTAPTSDISTSGFIYFAVVFLIIIVYLFFKNILFLFFFKRYPKNTPKIGVSNITTIAMIIAVAVSVVLVLMALAGGLTAALFRGYPGFRVTLELILVKISGLLFGPIIGIFSAATIDFLTVIFSGGVFNIGYVLGAILTGMIAGILREVLISTSFLNNKTLSDFAYLVLSVGMVFASFLVTQFFVISVTQNLSAFQSNDQIVLRFNASPLNFSISLQRYVQIIFYFAMVVIITMVVLYFV", "aa_hexdigest": "5e54c5af3e0ccf8a3d223961fe32b9b2", "start_type": "ATG", "rbs_motif": null, "genes": [ "gatC" ], "truncated": "3-prime", "pseudogene": { "start": 124384, "stop": 125424, "observations": { "mutation": [ 125119, 125341 ], "stop": [ 125119, 125341 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47344", "query_cov": 1.0, "subject_cov": 0.5136268343815513, "identity": 1.0, "score": 427.0, "evalue": 7.38e-147, "gene_start": 1, "gene_end": 245, "reference_start": 1, "reference_end": 245, "reference_sequence": "MGEIKNTAPTSDISTSGFIYFAVVFLIIIVYLFFKNILFLFFFKRYPKNTPKIGVSNITTIAMIIAVAVSVVLVLMALAGGLTAALFRGYPGFRVTLELILVKISGLLFGPIIGIFSAATIDFLTVIFSGGVFNIGYVLGAILTGMIAGILREVLISTSFLNNKTLSDFAYLVLSVGMVFASFLVTQFFVISVTQNLSAFQSNDQIVLRFNASPLNFSISLQRYVQIIFYFAMVVIITMVVLYFVWIIKQKHFNYAYSKFFFRRYKHANHQFTLFVLTKENWFYLILNVITLATTSLLMINIAFIPIFDTQTTGQTYDFWLLVRLLFAPLIFLLDIIVIYPILLLLTPIMLKGFKTVASETQTKGIKKSFSDMQSLIMPNVISHKKQQLIRKEMQQLAKTIRIDLSDKEVDALVEEFKEITKSFNKVTKIDTTNVQPMYAPFEFSPTPLRKDKPVVDKHAKQLLNNCCEVKTGFVKV" }, "paralog": false, "identity": 0.9942196531791907, "score": 655.0, "evalue": 5.28e-239, "description": "Internal stop codon at 125119, 125341. Nonsense mutation around 125119, 125341.", "psc": { "uniref90_id": "UniRef90_P47344", "gene": "gatC", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC", "uniref50_id": "UniRef50_P47344", "cog_id": "COG0721", "cog_category": "J", "go_ids": [ "GO:0005886", "GO:0006424", "GO:0006450", "GO:0030956", "GO:0050567", "GO:0070681" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47344", "UniRef:UniRef50_P47344", "COG:COG0721", "COG:J", "GO:0005886", "GO:0006424", "GO:0006450", "GO:0030956", "GO:0050567", "GO:0070681" ] }, "pscc": { "uniref50_id": "UniRef50_P47344", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47344" ], "product": "Uncharacterized protein MG098" } }, "id": "KMADJIBOEG_274", "locus": "GCA000292445_00235" }, { "type": "cds", "sequence": "contig_1", "start": 125209, "stop": 125343, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAACAAAAGAAAACTGGTTTTATCTAATTCTGAATGTAATTACATTAGCAACCACCAGTTTGCTAATGATTAACATTGCATTTATTCCTATCTTTGATACCCAAACAACTGGACAAACTTATGATTTTTGA", "aa": "MLTKENWFYLILNVITLATTSLLMINIAFIPIFDTQTTGQTYDF", "aa_hexdigest": "1ae888cae4e965623de8ebb0dcb8a1b2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5116.9391000000005, "isoelectric_point": 4.0500284194946286 }, "id": "KMADJIBOEG_275", "locus": "GCA000292445_00236" }, { "type": "cds", "sequence": "contig_1", "start": 125359, "stop": 125817, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGCTCCTTTGATCTTCTTACTTGATATTATTGTTATCTATCCAATCTTGTTATTGTTAACCCCAATCATGTTAAAAGGGTTTAAAACAGTAGCATCAGAAACCCAAACAAAAGGAATCAAAAAGAGTTTTTCAGATATGCAAAGCTTAATTATGCCCAATGTTATTAGTCACAAAAAACAGCAGTTAATTAGAAAAGAGATGCAACAGTTAGCAAAAACAATCAGAATTGATTTATCAGACAAAGAAGTGGATGCACTTGTTGAAGAGTTTAAAGAGATCACAAAGAGTTTTAATAAGGTAACTAAAATTGATACCACTAATGTTCAACCGATGTACGCTCCATTTGAATTTAGTCCAACCCCACTAAGAAAAGATAAACCAGTAGTTGATAAACACGCTAAGCAACTACTTAATAACTGTTGTGAAGTTAAAACAGGTTTTGTAAAGGTATAG", "aa": "MFAPLIFLLDIIVIYPILLLLTPIMLKGFKTVASETQTKGIKKSFSDMQSLIMPNVISHKKQQLIRKEMQQLAKTIRIDLSDKEVDALVEEFKEITKSFNKVTKIDTTNVQPMYAPFEFSPTPLRKDKPVVDKHAKQLLNNCCEVKTGFVKV", "aa_hexdigest": "2f66afd55144866347b09c9b0c48e245", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17382.546599999998, "isoelectric_point": 9.416332817077638 }, "id": "KMADJIBOEG_276", "locus": "GCA000292445_00237" }, { "type": "cds", "sequence": "contig_1", "start": 125820, "stop": 126629, "strand": "+", "frame": 3, "gene": "gatA", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA", "db_xrefs": [ "COG:COG0154", "COG:J", "EC:6.3.5.7", "GO:0005524", "GO:0006412", "GO:0006424", "GO:0030956", "GO:0050567", "SO:0001217", "UniRef:UniRef50_P47345", "UniRef:UniRef90_P47345" ], "nt": "GTGCGATCAAATATTTTAAGTCTCAGGGCGATACTTGATAAAAAACCTAGTGCCATTAACGATGTTTTAACATCAATTAATGCAAAGATAGAACTGAATAAATCAAGTAATTTTTTATTGAAAAATACTGTTGAAATTTATTCAAAAAAAATTAATAAAAGTGATGAAAAGATTCTGCTAAATAACATCCCTTATGTTTTGAAAGATAACATCGCTACTAAAGATATTGTCACCACTGGTGGTTCTTTGTTTTTAAAAAACTATCTTCCCCCTTTTTCAGCAACTGTGTTTGAACTGTTAGAAATGAATGGCGCGTTGCTTGTTGGTAAAGCTAATATGGATGAATTTGGCTTAGGTGGAACAGGTAGTTATTCTGCTTTTGGTGTTGTCCATCACCCTGAAAATTCCAGTTTAATTGCAGGTGGTTCTTCCTCAGGTTCAGCTTACGCAGTTGCTAAAGACATTGTTCCTTTTTCCATTGCAACTGACACTGGTGATTCGATTAGAAGACCTGCTAGTATCTGTAATGTTGTTGGCTTTAAACCAACTTATGGTTTGATATCACGTAATGGGGTATTTCCATATGCCCCAAGTATGGACCATGTGGGGATATTTGCTAAGTTTGTTAGTGATATTGCCATTGTTAGTGATGTTGTTATTAAACATGATAAAACTGATTTTTCTTCCCAAAAATCACCTGATGAAAACCAGTTTTTCAATGAGTTGGCCATTCCCTTTACAAGATCAATTCGCTTTGGTTATTTAAAACCACTAGAAAAACTGTTTAACAAACACCTCCAAAAAAAATGA", "aa": "MRSNILSLRAILDKKPSAINDVLTSINAKIELNKSSNFLLKNTVEIYSKKINKSDEKILLNNIPYVLKDNIATKDIVTTGGSLFLKNYLPPFSATVFELLEMNGALLVGKANMDEFGLGGTGSYSAFGVVHHPENSSLIAGGSSSGSAYAVAKDIVPFSIATDTGDSIRRPASICNVVGFKPTYGLISRNGVFPYAPSMDHVGIFAKFVSDIAIVSDVVIKHDKTDFSSQKSPDENQFFNELAIPFTRSIRFGYLKPLEKLFNKHLQKK", "aa_hexdigest": "cd92cb5d125a5d87cb7868e241d72642", "start_type": "GTG", "rbs_motif": null, "genes": [ "gatA" ], "truncated": "3-prime", "pseudogene": { "start": 125820, "stop": 126932, "observations": { "mutation": [ 126627 ], "stop": [ 126627 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47345", "query_cov": 1.0, "subject_cov": 0.5639412997903563, "identity": 1.0, "score": 517.0, "evalue": 7.86e-182, "gene_start": 1, "gene_end": 269, "reference_start": 1, "reference_end": 269, "reference_sequence": "MRSNILSLRAILDKKPSAINDVLTSINAKIELNKSSNFLLKNTVEIYSKKINKSDEKILLNNIPYVLKDNIATKDIVTTGGSLFLKNYLPPFSATVFELLEMNGALLVGKANMDEFGLGGTGSYSAFGVVHHPENSSLIAGGSSSGSAYAVAKDIVPFSIATDTGDSIRRPASICNVVGFKPTYGLISRNGVFPYAPSMDHVGIFAKFVSDIAIVSDVVIKHDKTDFSSQKSPDENQFFNELAIPFTRSIRFGYLKPLEKLFNKHLQKKWNNLKKTLEQKNYQLIPLDFDVELLKVIDSIYKIISYSEAVSCYSNLTGIVFGQKVFEPNSPSNFDQTITRNRDQFLGKQLKRRFVIGAFATDEKNFEKYFEKAQKIRRVLVDNFLNLFSDVDFVLSPSASCFASTIEDIQANKPYTNIIDDFLQLANFAGSPSITIPWLVQTKDQTIGLSISANCFEDKKLLQIAYWFEQLFDLNHD" }, "paralog": false, "identity": 0.9945945945945946, "score": 724.0, "evalue": 7.84e-266, "description": "Internal stop codon at 126627. Nonsense mutation around 126627.", "psc": { "uniref90_id": "UniRef90_P47345", "gene": "gatA", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA", "ec_ids": [ "6.3.5.7" ], "uniref50_id": "UniRef50_P47345", "cog_id": "COG0154", "cog_category": "J", "go_ids": [ "GO:0005524", "GO:0006412", "GO:0006424", "GO:0030956", "GO:0050567" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47345", "EC:6.3.5.7", "UniRef:UniRef50_P47345", "COG:COG0154", "COG:J", "GO:0005524", "GO:0006412", "GO:0006424", "GO:0030956", "GO:0050567" ] }, "pscc": { "uniref50_id": "UniRef50_P47345", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47345" ], "product": "Glutamyl-tRNA(Gln) amidotransferase subunit A" } }, "id": "KMADJIBOEG_277", "locus": "GCA000292445_00238" }, { "type": "cds", "sequence": "contig_1", "start": 126669, "stop": 127220, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01425.27" ], "nt": "TTGATTCCACTTGATTTTGATGTGGAACTTCTCAAAGTAATTGATTCTATTTACAAAATAATTAGTTATAGCGAAGCAGTTAGTTGTTATAGTAATTTAACTGGCATTGTCTTTGGTCAAAAGGTGTTTGAACCTAATTCACCAAGTAATTTTGATCAAACTATTACCAGAAACAGAGATCAGTTTTTAGGTAAACAACTAAAAAGAAGATTTGTAATAGGGGCATTTGCAACTGATGAGAAGAATTTTGAAAAGTACTTTGAAAAAGCTCAGAAAATAAAAAGAGTCTTAGTGGATAACTTTCTGAATCTCTTTAGTGATGTTGATTTTGTATTATCACCAAGCGCTTCTTGTTTTGCTAGTACCATTGAAGATATTCAAGCTAATAAGCCATATACAAACATTATTGATGACTTTTTACAATTAGCTAATTTTGCTGGTAGCCCTTCTATAACTATCCCTTGGTTAGTTCAAACAAAAGACCAAACAATTGGTTTAAGTATTAGTGCTAACTGTTTTGAAGATAAAAAACTCTTACAAATTGCTTATTGA", "aa": "MIPLDFDVELLKVIDSIYKIISYSEAVSCYSNLTGIVFGQKVFEPNSPSNFDQTITRNRDQFLGKQLKRRFVIGAFATDEKNFEKYFEKAQKIKRVLVDNFLNLFSDVDFVLSPSASCFASTIEDIQANKPYTNIIDDFLQLANFAGSPSITIPWLVQTKDQTIGLSISANCFEDKKLLQIAY", "aa_hexdigest": "9dee6af06d7c69a49770305182d97c4f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01425.27", "name": "Amidase", "length": 180, "aa_cov": 0.9562841530054644, "hmm_cov": 0.9555555555555556, "evalue": 1.4335393881009705e-30, "score": 101.78336334228516, "start": 5, "stop": 179 } ], "seq_stats": { "molecular_weight": 20779.509199999997, "isoelectric_point": 5.032376670837402 }, "id": "KMADJIBOEG_278", "locus": "GCA000292445_00239" }, { "type": "cds", "sequence": "contig_1", "start": 127246, "stop": 128679, "strand": "+", "frame": 1, "gene": "gatB", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB", "db_xrefs": [ "COG:COG0064", "COG:J", "EC:6.3.5.-", "GO:0005524", "GO:0006412", "GO:0006424", "GO:0016884", "GO:0030956", "GO:0050566", "GO:0050567", "GO:0070681", "KEGG:K02434", "RefSeq:WP_014894150.1", "SO:0001217", "UniParc:UPI00027B387B", "UniRef:UniRef100_UPI00027B387B", "UniRef:UniRef50_P75533", "UniRef:UniRef90_P47346" ], "nt": "ATGATTAATTTTGAAGCGATTATTGGAATTGAAGTCCATGTAGTTTTAAATACTGCTAGCAAGATGTTTTCAAAAGCACCTAACCGCGTTGATAATCAAAAAATCAATCATTTTATTGACCCAATAGATTTAGGTTTACCAGGCACTTTGCCTCAAGTTAATGAGTTAGCAGTTTACAAGGCATTATTATTAGCTGATGCATTAAAAATGAAGACAGTAACAAATAAACTTGTTTTTGATCGAAAGCACTATTTTTATCAGGACTTACCTAAGGGTTTTCAAATCACCCAACAAAACTATCCTTTTGCTAAAAATGGTGTAGTTACCATTAATGTTGATGCTATTGAAAAACCAATTTATATTGACCGGTTTCATTTGGAGGAAGATACTGCCAAACAACACTTTAACCATGACCAAATTCTGCTTGATTTTAATAGGTGTGGTGCACCTTTAATTGAAGTTGTTACTCTTCCTGTTATCAACACTGCAAAAGAAGCGAAAGCCTACCTACAGAAGTTGAGACAAATTCTGATTGTTAACAATATCTCCAATGCCAAATTGGAAGATGGTTCAATGCGGAGCGATTGCAATGTTTCAGTACGTTTAAAAGGTCAAAGGCAACTAGGAACTAAAATTGAAATTAAAAACATCAACTCACTTAATAATGTTGAAAAAGCGATTGATCTTGAGATTAACCGCCAAGTTAAAGCACTTATTAATGGTGAAACCTTGAGTCAAGCAACTTTAAGCTTTGATGATAAAACCAACAACAATGTTTTTATGAGAAAAAAAGACAATACGATTGACTATAGGTACTTTATTGAACCTAACATCATGACTAGTAATATTGATGATTTATTATTAGAAAAACCTGTTGCTTTTCAGTTAGAACAGTTTCAAAAAGAACTAATTGATAGTAATGTCAATCCTCAATTAGTCCAATTAGTAGTTGATGATGCAACTATCTTTAGTGCTTTTCAAACTATTAATAGGGTGGTTCAAAACCCCCAAGAAACCATCAGGTGGTTATGTATTGAACTAATGGGTCAACTCAATAAAACCAACAGTTCATTAACAGTTAATTTAATTCAAGATCTAATTACCCTAATTGAAATGCTAAAAGCAGCAAAGGTTAACCAAAAACAAGCTAAGCAGTTAATTACTTTAATGATTGAAACTAAAAAAGATCCAAAATCGCTAGCTAAGCTCCATAATTTAGAGCAAATTACTGATCCAAAAGAGTTACAAAAGATCATTAAAAAAATCTTTCAGGAAAATGAAAGAGAGATCCTGAAAAACATTGATAGAATTGAACGGATTCAAAAGTTAATTATTGGTCAAGTTATGCATAAGACCAATAATCGAGCAAACCCCCAACAAGTTTTTATAATTGTTGAAAATATGCTTCATGAAGTTCGGGAAAGAGATAGCTAA", "aa": "MINFEAIIGIEVHVVLNTASKMFSKAPNRVDNQKINHFIDPIDLGLPGTLPQVNELAVYKALLLADALKMKTVTNKLVFDRKHYFYQDLPKGFQITQQNYPFAKNGVVTINVDAIEKPIYIDRFHLEEDTAKQHFNHDQILLDFNRCGAPLIEVVTLPVINTAKEAKAYLQKLRQILIVNNISNAKLEDGSMRSDCNVSVRLKGQRQLGTKIEIKNINSLNNVEKAIDLEINRQVKALINGETLSQATLSFDDKTNNNVFMRKKDNTIDYRYFIEPNIMTSNIDDLLLEKPVAFQLEQFQKELIDSNVNPQLVQLVVDDATIFSAFQTINRVVQNPQETIRWLCIELMGQLNKTNSSLTVNLIQDLITLIEMLKAAKVNQKQAKQLITLMIETKKDPKSLAKLHNLEQITDPKELQKIIKKIFQENEREILKNIDRIERIQKLIIGQVMHKTNNRANPQQVFIIVENMLHEVRERDS", "aa_hexdigest": "6423815809bcc7bb1738a2171651ca7b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B387B", "ncbi_nrp_id": "WP_014894150.1", "uniref100_id": "UniRef100_UPI00027B387B", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B387B", "RefSeq:WP_014894150.1", "UniRef:UniRef100_UPI00027B387B" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B387B", "uniref90_id": "UniRef90_P47346" }, "psc": { "uniref90_id": "UniRef90_P47346", "gene": "gatB", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB", "ec_ids": [ "6.3.5.-" ], "uniref50_id": "UniRef50_P75533", "cog_id": "COG0064", "cog_category": "J", "kegg_orthology_id": "K02434", "go_ids": [ "GO:0005524", "GO:0006412", "GO:0006424", "GO:0016884", "GO:0030956", "GO:0050566", "GO:0050567", "GO:0070681" ] }, "pscc": { "uniref50_id": "UniRef50_P75533", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75533" ], "product": "Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B" }, "genes": [ "gatB" ], "id": "KMADJIBOEG_279", "locus": "GCA000292445_00240" }, { "type": "cds", "sequence": "contig_1", "start": 128873, "stop": 129322, "strand": "+", "frame": 2, "gene": "mngR", "product": "putative HTH-type transcriptional regulator MG101", "db_xrefs": [ "COG:COG2188", "COG:K", "GO:0003677", "GO:0003700", "SO:0001217", "UniRef:UniRef50_P75532", "UniRef:UniRef90_P47347" ], "nt": "GTGTGTAAAGATGTAGGCTTTAGTTTTTTTCATAAAACCCAGGATAATCTGAAGGTGAAATATGCTAAACTTTCAACTTTGATTAAAAAGCTGTTATCACAGGATGATGCTAGTGTTTTTGCCAATATTGACAGTACTGTGCACCTTGATAAATTTAAGGGAATTGAAGCTAAGTTTTTTGATGAGAATAAAAAACACTTTTTAAACGTTTGTTTTTTTGCTAAAGATGATATTTTAAACATCCTTGATGAAAACAACCTCCAGCAACAGTTCTTCAGGGAGTTTGCTTACAATGGGATTGCTATTGAAAAACGTCATTGTGTTACCTCTGTTGACAAGGAAAGTGGGTGCTTGGTGATGTATGACATGTATTATGATGACAATGATAATTTTGTAGTTGCTAGCAAAAGTAACTTCCTTAACCCTGAATTGAAAGTAATCAATGCTTAA", "aa": "MCKDVGFSFFHKTQDNLKVKYAKLSTLIKKLLSQDDASVFANIDSTVHLDKFKGIEAKFFDENKKHFLNVCFFAKDDILNILDENNLQQQFFREFAYNGIAIEKRHCVTSVDKESGCLVMYDMYYDDNDNFVVASKSNFLNPELKVINA", "aa_hexdigest": "0a610266e88f07daa4cc4fff18420b8c", "start_type": "GTG", "rbs_motif": null, "genes": [ "mngR" ], "truncated": "5-prime", "pseudogene": { "start": 128654, "stop": 129322, "observations": { "mutation": [ 128732 ], "stop": [ 128732 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47347", "query_cov": 1.0, "subject_cov": 0.6711711711711712, "identity": 0.993, "score": 302.0, "evalue": 4.93e-103, "gene_start": 1, "gene_end": 149, "reference_start": 74, "reference_end": 222, "reference_sequence": "MKFGKEIAKKNQIIYRYIILKIQSFEWPANTRIFSERQLEIRFNSSRSQIRSVLATLLNKNIIRYTKNTPGYFVCKDVGFSFFHKTQDNLKVKYAKLSTLIKKLLSQDDASVFANIDSTVHLDKFKGIEAKFFDENKKHFLNVCFFAKDDILNILDENNLQQQFFREFAYNGIAIEKRHCVTSVDKESGCLVMYDMYYDDNDNFVVASKSNFLNPELKVINA" }, "paralog": false, "identity": 0.9954954954954955, "score": 442.0, "evalue": 1.69e-160, "description": "Internal stop codon at 128732. Nonsense mutation around 128732.", "psc": { "uniref90_id": "UniRef90_P47347", "gene": "MngR", "product": "Uncharacterized HTH-type transcriptional regulator MG101", "uniref50_id": "UniRef50_P75532", "cog_id": "COG2188", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0003700" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47347", "UniRef:UniRef50_P75532", "COG:COG2188", "COG:K", "GO:0003677", "GO:0003700" ] }, "pscc": { "uniref50_id": "UniRef50_P75532", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75532" ], "product": "Uncharacterized HTH-type transcriptional regulator MG101 homolog" } }, "id": "KMADJIBOEG_280", "locus": "GCA000292445_00241" }, { "type": "cds", "sequence": "contig_1", "start": 129315, "stop": 130262, "strand": "+", "frame": 3, "gene": "trxB", "product": "thioredoxin-disulfide reductase", "db_xrefs": [ "COG:COG0492", "COG:O", "EC:1.8.1.9", "GO:0004791", "GO:0005737", "GO:0019430", "GO:0045454", "GO:0071949", "RefSeq:WP_009885660.1", "SO:0001217", "UniParc:UPI00001376C6", "UniRef:UniRef100_P47348", "UniRef:UniRef50_P47348", "UniRef:UniRef90_P47348" ], "nt": "ATGCTTAAAGTGAATGCTGATTTTTTAACTAAAGATCAAGTTATCTATGATTTAGTGATAGTAGGTGCTGGCCCTGCTGGGATTGCTAGTGCCATTTATGGTAAACGTGCTAACTTAAATTTAGCAATTATTGAAGGAAACACTCCAGGAGGGAAGATAGTAAAAACTAACATTGTGGAAAACTATCCTGGTTTTAAAACCATAACTGGTCCTGAATTAGGTCTTGAGATGTACAACCACTTGTTAGCATTTGAACCAGTTGTTTTTTATAACAACTTAATCAAAATTGATCATCTTAACGATACATTCATCTTGTATTTAGATAACAAAACGACAGTTTTTAGCAAAACTGTTATCTATGCAACAGGGATGGAAGAGAGAAAACTTGGCATTGAAAAGGAAGATTATTTTTATGGTAAAGGGATTAGTTATTGTGCTATTTGTGATGCGGCTCTTTACAAAGGTAAAACAGTTGGTGTTGTAGGAGGAGGTAATTCTGCAATACAGGAAGCAATTTATCTTTCAAGTATTGCTAAAACAGTTCACCTTATTCACAGACGTGAAGTGTTTAGAAGTGATGCATTACTAGTTGAAAAATTAAAAAAAATTAGTAATGTAGTTTTTCATTTAAATGCTACTGTAAAACAGTTAATAGGTCAAGAAAAGCTCCAAACTGTTAAATTGGCAAGCACAGTTGATAAATCAGAAAGTGAAATTGCAATTGATTGTCTCTTTCCTTACATAGGCTTTGAAAGTAATAACAAGCCAGTTTTAGATCTTAAGCTTAATTTAGATCAAAATGGTTTTATTTTAGGAGATGAAAATATGCAAACTAACATTAAGGGTTTTTATGTTGCTGGGGATTGTAGAAGTAAATCATTCCGGCAAATTGCCACTGCAATTAGTGATGGGGTAACAGCTGTTTTAAAGGTTAGGGATGACATTTAG", "aa": "MLKVNADFLTKDQVIYDLVIVGAGPAGIASAIYGKRANLNLAIIEGNTPGGKIVKTNIVENYPGFKTITGPELGLEMYNHLLAFEPVVFYNNLIKIDHLNDTFILYLDNKTTVFSKTVIYATGMEERKLGIEKEDYFYGKGISYCAICDAALYKGKTVGVVGGGNSAIQEAIYLSSIAKTVHLIHRREVFRSDALLVEKLKKISNVVFHLNATVKQLIGQEKLQTVKLASTVDKSESEIAIDCLFPYIGFESNNKPVLDLKLNLDQNGFILGDENMQTNIKGFYVAGDCRSKSFRQIATAISDGVTAVLKVRDDI", "aa_hexdigest": "c62ad9a7e9d36b17e652e480cf0c7955", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001376C6", "ncbi_nrp_id": "WP_009885660.1", "uniref100_id": "UniRef100_P47348", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001376C6", "RefSeq:WP_009885660.1", "UniRef:UniRef100_P47348" ] }, "ips": { "uniref100_id": "UniRef100_P47348", "uniref90_id": "UniRef90_P47348" }, "psc": { "uniref90_id": "UniRef90_P47348", "gene": "trxB", "product": "thioredoxin-disulfide reductase", "ec_ids": [ "1.8.1.9" ], "uniref50_id": "UniRef50_P47348", "cog_id": "COG0492", "cog_category": "O", "go_ids": [ "GO:0004791", "GO:0005737", "GO:0019430", "GO:0045454", "GO:0071949" ] }, "pscc": { "uniref50_id": "UniRef50_P47348", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47348" ], "product": "Thioredoxin reductase" }, "genes": [ "trxB" ], "id": "KMADJIBOEG_281", "locus": "GCA000292445_00242" }, { "type": "cds", "sequence": "contig_1", "start": 130384, "stop": 130989, "strand": "+", "frame": 1, "gene": "whiA", "product": "DNA-binding protein WhiA", "db_xrefs": [ "COG:COG1481", "COG:K", "GO:0003677", "GO:0043937", "GO:0051301", "SO:0001217", "UniRef:UniRef50_P47349", "UniRef:UniRef90_P47349" ], "nt": "GTGCAATCTGAAGCATTAAAGGAACAATTTGTTCAAAATCTTAAGTTTGACTTTAAGACAAAAGCTAGTAAGAAATACTTTCTTTTTGAGTTTAATGCAGATATTAACGTAATTAACACTCTTTTAAAACTTGATGTGACAACTAGTGAATTGGTAGTTAAACAAGTTTATCTCATTGCTGCTTTTTTAAGTGGAGGTAGTGTTAGTGATTTAATAAACTCCAATAACTTTCACTTGCAAATCAGTTCCAACAATGAGTTTCAAATTCAACAACTTTTAAAATTGTTTAGTTTTTTTAAAAAAACAGTTAAACAAAACCAGTTAGTTGTTTATCTTAAAAGTTATGAAAAGATCTGTAATTTTTTAAAACTGATTCAAGCCTTTGATGGTTATCTTGCTTTTGAAAATAAGCAACTAGAGAAAAGTTTTACTTTAAACCAGTTAAGAAAAAGTAATTTGGAAGTTGCTAACTTAATGAAGACAATCAGATCTAATAATCAAACTAATCAACTCCAACTAAAATCATTTATTAAAAGTAGTAGTTTTGCAAAAAGACCGCTTAATTTTCAGCGTTATTGCTTAATTAAAAGTGATCATCCTGATTGA", "aa": "MQSEALKEQFVQNLKFDFKTKASKKYFLFEFNADINVINTLLKLDVTTSELVVKQVYLIAAFLSGGSVSDLINSNNFHLQISSNNEFQIQQLLKLFSFFKKTVKQNQLVVYLKSYEKICNFLKLIQAFDGYLAFENKQLEKSFTLNQLRKSNLEVANLMKTIRSNNQTNQLQLKSFIKSSSFAKRPLNFQRYCLIKSDHPD", "aa_hexdigest": "30fc90082c1df06ba3d8cd002482d3ca", "start_type": "GTG", "rbs_motif": null, "genes": [ "whiA" ], "truncated": "both", "pseudogene": { "start": 130252, "stop": 131094, "observations": { "mutation": [ 130312, 130372, 130987 ], "stop": [ 130312, 130372, 130987 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47349", "query_cov": 1.0, "subject_cov": 0.7178571428571429, "identity": 0.995, "score": 376.0, "evalue": 1.77e-130, "gene_start": 1, "gene_end": 201, "reference_start": 45, "reference_end": 245, "reference_sequence": "MTFSTQIKAELVQNKLIDKHWNVFLAGFFQNNLKLLYNRNWSFKVQSEALKEQFVQNLKFDFKTKASKKYFLFEFNADINVINTLLKLDVTTSELVVKQVYLIAAFLSGGSVSDLINSNNFHLQISSNNEFQIQQLLKLFSFFKKTVKQNQLVVYLKSYEKICNFLKLIQAFDGYLAFENKQLEKSFTLNQLRKSNLEVANLMKTIRSNNQTNQLQLKSFIKSSSFAKRPLNFQRYCLIKSDHPDWSLEQIANFFFTKYNIKISRSGIQHFSVNLKKLCQ" }, "paralog": false, "identity": 0.9892857142857143, "score": 536.0, "evalue": 7.39e-196, "description": "Internal stop codon at 130312, 130372, 130987. Nonsense mutation around 130312, 130372, 130987.", "psc": { "uniref90_id": "UniRef90_P47349", "gene": "whiA", "product": "DNA-binding protein WhiA", "uniref50_id": "UniRef50_P47349", "cog_id": "COG1481", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0043937", "GO:0051301" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47349", "UniRef:UniRef50_P47349", "COG:COG1481", "COG:K", "GO:0003677", "GO:0043937", "GO:0051301" ] }, "pscc": { "uniref50_id": "UniRef50_P47349", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47349" ], "product": "Probable cell division protein WhiA" } }, "id": "KMADJIBOEG_282", "locus": "GCA000292445_00243" }, { "type": "cds", "sequence": "contig_1", "start": 131116, "stop": 131349, "strand": "+", "frame": 1, "gene": "secG", "product": "preprotein translocase subunit SecG", "db_xrefs": [ "GO:0005886", "GO:0009306", "GO:0015136", "GO:0015450", "GO:0015739", "GO:0016020", "RefSeq:WP_010869333.1", "SO:0001217", "UniParc:UPI00001357BA", "UniRef:UniRef100_P58061", "UniRef:UniRef50_P58061", "UniRef:UniRef90_P58061" ], "nt": "ATGCATCCAATCCAAATAGTAATGTTCATTATGGCTGTTATCTGTTTAATTATTGGACTTTTGCTTTCTAACCATGGTTCTACTGGAGGATTAGCTTCTCTATCAGGTCAGGACTTGGAAATCTTTCGTAAAACCAAAGATAGGGGTTTTGTAAAGATCTTACAGATTATCATGTTTATCTTAGTAGTTTTATTTTTAATTCTTGGGTTGATATTTAGTTTTGCACCAAGATAA", "aa": "MHPIQIVMFIMAVICLIIGLLLSNHGSTGGLASLSGQDLEIFRKTKDRGFVKILQIIMFILVVLFLILGLIFSFAPR", "aa_hexdigest": "ae937ae76cf05dd412b9491e9bfeb245", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001357BA", "ncbi_nrp_id": "WP_010869333.1", "uniref100_id": "UniRef100_P58061", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001357BA", "RefSeq:WP_010869333.1", "UniRef:UniRef100_P58061" ] }, "ips": { "uniref100_id": "UniRef100_P58061", "uniref90_id": "UniRef90_P58061" }, "psc": { "uniref90_id": "UniRef90_P58061", "gene": "secG", "product": "preprotein translocase subunit SecG", "uniref50_id": "UniRef50_P58061", "go_ids": [ "GO:0005886", "GO:0009306", "GO:0015136", "GO:0015450", "GO:0015739", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P58061", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P58061" ], "product": "Probable protein-export membrane protein SecG" }, "genes": [ "secG" ], "id": "KMADJIBOEG_283", "locus": "GCA000292445_00244" }, { "type": "cds", "sequence": "contig_1", "start": 131352, "stop": 131513, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGTTCTAACTGAACTCCAAAAGCAGATATTTACCATTGTCAAAAAGGAAAATGGTAAACCTATTCCCCCTGGAATAGTGGTAAGAATGATGGAAAATAGTCCTAATTTTCCAGGTAAACATCTCATCTATCGGGCCATTGATGATCTGCTTGATTGA", "aa": "MKVLTELQKQIFTIVKKENGKPIPPGIVVRMMENSPNFPGKHLIYRAIDDLLD", "aa_hexdigest": "5a38b02da334b0cefc910e0643653a80", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6077.189500000001, "isoelectric_point": 9.22350788116455 }, "id": "KMADJIBOEG_284", "locus": "GCA000292445_00245" }, { "type": "cds", "sequence": "contig_1", "start": 131646, "stop": 132932, "strand": "+", "frame": 3, "gene": "rnr", "product": "ribonuclease R", "db_xrefs": [ "COG:COG0557", "COG:K", "EC:3.1.13.1", "GO:0003723", "GO:0004518", "GO:0005829", "GO:0006402", "GO:0008859", "KEGG:K12573", "SO:0001217", "UniRef:UniRef50_P47350", "UniRef:UniRef90_P47350" ], "nt": "TTGGATGATGATAAAACTGTGTATTATGTCCATTACTCTAATTTAACTGGAGCTTTAGATGGGGATCTTGTGGAGTTTTGTAAATTAGATAAACCCCAATTTGGTGATAAGTTTGATGCTGCAGTTATTACTATTCTAAAAAGAGCAAGAATCTTGTATGCAGGTAATTTTTTAGTAGATCAAAATGAGTTTGCCTTGGAATACAAAATTGTTGCTGATAACCCTAGATTTTATTTAACTACGATTGTAAATCCTGATTCTATCCCAAATAACTTAGCATCTAACACCAAGATAGCTTTTCAAATTGATGAGTATGATCCTGATAACAACTTATGTAAGGTTTCTGTACAACAAGTTTTGGGTAACAATGATGATCCGCTAATTAATATAAAAGCAATCATGTTGGACAATTCCATTGTCTTTGAAACTAACGATGTAGTTGAACAGCATGCTAACAAGTTAAGTTTTGATACTGAAGAACAACATAAAGCTTACCGTCAGGATTTAACTGATTTAGCTTTTGTGACTGTTGATCCTACAACATCAAAAGACCTTGATGATGCTATTTATGTCAAAACAATACCAACAGGTTTTGTGCTTTATGTAGCTATTGCTGATGTTGCACACTATGTTAATAGAAATAGTGAAATAGACATTGAAGCAAAACACAAAACAAGCTCAATCTATCTACCTGGTCATTATGTTGTGCCCATGCTACCTGAGCAATTGTCAAATCAGCTCTGTTCTTTAAATCCAGCACAAAAACGTTATGTTGTTGTTTGTGAGATTAGTTTTGATAATCAGGGAAGGATTAAAACAAACAAGCTTTACCCAGCAACAATTATTTCCAAAAATCGTTTTAGCTATGATCAGGTTAACAAGTGGTTAAATAATAAATCAGAATTAAACTGTGATGAAACAGTTATCAACAGCTTAAAAGCAGCTTTTACACTAAGTGATCTAATTCAAGCGCAACGTCAAAAACGCGGTACAATTGATCTTTCACACAAAGAAACTGAGATAGTTGTTGATGAACATTATTTTCCCATTAAGATAAATTTTTTGGTTCACGATAAAGCTGAAACCATGATTGAAAATCTCATGGTAGTGGCCAATGAGACAGTTGCTTGGGTGTTAACTAACAACAAAATTGCTTTACCATACAGAGTTCACCCAAGACCAAGCAAAAAGAAGTTACAAAGTTTGATTGAAACAGTTGGTGAGTTGAACATAACTAAACCCCAATTTAACTTAGATACTGTCACTTCAAGCCAAATAGCTAGCTGA", "aa": "MDDDKTVYYVHYSNLTGALDGDLVEFCKLDKPQFGDKFDAAVITILKRARILYAGNFLVDQNEFALEYKIVADNPRFYLTTIVNPDSIPNNLASNTKIAFQIDEYDPDNNLCKVSVQQVLGNNDDPLINIKAIMLDNSIVFETNDVVEQHANKLSFDTEEQHKAYRQDLTDLAFVTVDPTTSKDLDDAIYVKTIPTGFVLYVAIADVAHYVNRNSEIDIEAKHKTSSIYLPGHYVVPMLPEQLSNQLCSLNPAQKRYVVVCEISFDNQGRIKTNKLYPATIISKNRFSYDQVNKWLNNKSELNCDETVINSLKAAFTLSDLIQAQRQKRGTIDLSHKETEIVVDEHYFPIKINFLVHDKAETMIENLMVVANETVAWVLTNNKIALPYRVHPRPSKKKLQSLIETVGELNITKPQFNLDTVTSSQIAS", "aa_hexdigest": "075840184efc42bb117eabe3f322546e", "start_type": "TTG", "rbs_motif": null, "genes": [ "rnr" ], "truncated": "both", "pseudogene": { "start": 131352, "stop": 133235, "observations": { "mutation": [ 131511, 132930, 133113 ], "stop": [ 131511, 132930, 133113 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47350", "query_cov": 1.0, "subject_cov": 0.5729585006693441, "identity": 0.993, "score": 840.0, "evalue": 2.53e-302, "gene_start": 1, "gene_end": 428, "reference_start": 121, "reference_end": 548, "reference_sequence": "MGHHHHHHHHHHSSGHIDDDDKMKVLTELQKQIFTIVKKENGKPIPPGIVVRMMENSPNFPGKHLIYRAIDDLLDWAILRKAGGVTNQLLVNYEPAEPLLDKKLQGILTLGNKNSGFIRSLDDDKTVYYVHYSNLTGALDGDLVEFCKLDKPQFGDKFDAAVITILKRARILYAGNFLVDQNEFALEYKIVADNPRFYLTMIVNPDSIPNNLASNTKIAFQIDEYDPDNNLCKVSVQQVLGNNDDPLINIKAIMLDNSIVFETNDVVEQHANKLSFDTEEQHKAYRQDLTDLAFVTVDPTTSKDLADAIYVKTIPTGFVLYVAIADVAHYVNRNSEIDIEAKHKTSSIYLPGHYVVPMLPEQLSNQLCSLNPAQKRYVVVCEISFDNQGRIKTNKLYPATIISKNRFSYDQVNKWLNNKSELNCDETVINSLKAAFTLSDLIQAQRQKRGTIDLSHKETEIVVDEHYFPIKINFLVHDKAETMIENLMVVANETVAWVLTNNKIALPYRVHPRPSKKKLQSLIETVGELNITKPQFNLDTVTSSQIASWLNENKDNPSYEIFVILLLRTLGKAFYSVNPLMHFSIGSNHYTHFTSPIRRYIDLTIHRLLWMHLFTPDQFTDNERDQLKQELEKIADTVNDTEIKIINCERNANDYLTTLLLSKQIGKTFSGFISAITSFGIFMRMDENNFDGLIKITTIPDDFFIFEKEKMVLKGRKTNKVYKIGDRLEAKLSEIDFIQKRAILTLI" }, "paralog": false, "identity": 0.9920255183413078, "score": 1236.0, "evalue": 0.0, "description": "Internal stop codon at 131511, 132930, 133113. Nonsense mutation around 131511, 132930, 133113.", "psc": { "uniref90_id": "UniRef90_P47350", "gene": "rnr", "product": "ribonuclease R", "ec_ids": [ "3.1.13.1" ], "uniref50_id": "UniRef50_P47350", "cog_id": "COG0557", "cog_category": "K", "kegg_orthology_id": "K12573", "go_ids": [ "GO:0003723", "GO:0004518", "GO:0005829", "GO:0006402", "GO:0008859" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47350", "EC:3.1.13.1", "UniRef:UniRef50_P47350", "COG:COG0557", "COG:K", "KEGG:K12573", "GO:0003723", "GO:0004518", "GO:0005829", "GO:0006402", "GO:0008859" ] }, "pscc": { "uniref50_id": "UniRef50_P47350", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47350" ], "product": "Ribonuclease R" } }, "id": "KMADJIBOEG_285", "locus": "GCA000292445_00246" }, { "type": "cds", "sequence": "contig_1", "start": 133116, "stop": 133529, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00575.29" ], "nt": "ATGCATCTTTTTACTCCCGATCAATTCACTGATAATGAAAGAGATCAACTCAAACAAGAGTTGGAAAAAATTGCTGATACAGTTAATGATACAGAGATTAAAATTATCAATTGTGAAAGAAATGCCAATGATTATCTAACAACGCTGTTATTATCAAAACAAATTGGCAAAACCTTCAGCGGATTTATTTCAGCAATTACTAGCTTTGGAATTTTTATGAGAATGGATGAAAATAACTTTGATGGGTTAATCAAAATTACAACTATCCCTGATGTTTTCTTTATTTTTGAAAAGGAAAAAATGGTATTGAAAGGAAGAAAAACTAATAAGGTTTATAAAATTGGCGATCGTTTGGAAGCTAAACTAAGTGAGATTGATTTCATCCAAAAACGTGCTATTTTAACACTCATATAA", "aa": "MHLFTPDQFTDNERDQLKQELEKIADTVNDTEIKIINCERNANDYLTTLLLSKQIGKTFSGFISAITSFGIFMRMDENNFDGLIKITTIPDVFFIFEKEKMVLKGRKTNKVYKIGDRLEAKLSEIDFIQKRAILTLI", "aa_hexdigest": "ae31d39efbebf73e4520149cd737b7f8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00575.29", "name": "S1", "length": 82, "aa_cov": 0.5985401459854015, "hmm_cov": 0.8536585365853658, "evalue": 2.4937909026156997e-11, "score": 39.01045227050781, "start": 54, "stop": 135 } ], "seq_stats": { "molecular_weight": 15902.247299999988, "isoelectric_point": 5.884279441833496 }, "id": "KMADJIBOEG_286", "locus": "GCA000292445_00247" }, { "type": "cds", "sequence": "contig_1", "start": 133537, "stop": 133653, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGTTAATATCCTGCTTTTTATTACCCTTATTTTTTTACTTTTACTGTTTGTTTTTTTAATTGCTTTTGCATTTCTAAACAAACGGGTTAGAAATTATGTTGTACGTACTTGA", "aa": "MVVNILLFITLIFLLLLFVFLIAFAFLNKRVRNYVVRT", "aa_hexdigest": "e7bad74b7219d04b6fd7a3731452fcfd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4544.662900000002, "isoelectric_point": 11.722947120666507 }, "id": "KMADJIBOEG_287", "locus": "GCA000292445_00248" }, { "type": "cds", "sequence": "contig_1", "start": 133888, "stop": 134139, "strand": "+", "frame": 1, "gene": "cdaM", "product": "diadenylate cyclase CdaM", "db_xrefs": [ "COG:COG1624", "COG:T", "EC:2.7.7.85", "GO:0004016", "GO:0005524", "GO:0005886", "GO:0006171", "GO:0106408", "KEGG:K18672", "SO:0001217", "UniRef:UniRef50_P47351", "UniRef:UniRef90_P47351" ], "nt": "GTGATTGTTAGAGATTACAAAATTATTAGTGTCTCTTCTTACTTTCCAATGACAAGACAATTAATTGATGTTTCATATGGTTCAAGACACAGAAGTGCCTTGGGATTAAGTGAAAAGTCTGATGCAGTTGTTTTTATTGTTAGTGAAACAACTGGTAAAATATCAGTTGCTTTAAAAGGGGTTATTAAAACCTTGTCTTCCAACTCAGATAGGTTGCAAGATGAAATTATCCATTACTTGTCTTCAAAGTAG", "aa": "MIVRDYKIISVSSYFPMTRQLIDVSYGSRHRSALGLSEKSDAVVFIVSETTGKISVALKGVIKTLSSNSDRLQDEIIHYLSSK", "aa_hexdigest": "5729fce41ac549b731dc69104e4ea5cb", "start_type": "GTG", "rbs_motif": null, "genes": [ "cdaM" ], "truncated": "5-prime", "pseudogene": { "start": 133594, "stop": 134139, "observations": { "mutation": [ 133651 ], "stop": [ 133651 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47351", "query_cov": 0.9879518072289156, "subject_cov": 0.41, "identity": 1.0, "score": 155.0, "evalue": 2.94e-46, "gene_start": 2, "gene_end": 83, "reference_start": 119, "reference_end": 200, "reference_sequence": "MVVNILLFITLIFLLLLFVFLIAFAFLNKRVRNYVVRTWTSVFSKSKQNLDKKNFFDNLTSTLLRLSVDKIGAIIAIEKRDSLDPYINIGYRVSSDFSPELLVTIFYNKSSPLHDGAVIVRDYKIISVSSYFPMTRQLIDVSYGSRHRSALGLSEKSDAVVFIVSETTGKISVALKGVIKTLSSNSDRLQDEIIHYLSSK" }, "paralog": false, "identity": 0.994475138121547, "score": 343.0, "evalue": 4.06e-123, "description": "Internal stop codon at 133651. Nonsense mutation around 133651.", "psc": { "uniref90_id": "UniRef90_P47351", "gene": "cdaM", "product": "diadenylate cyclase CdaM", "ec_ids": [ "2.7.7.85" ], "uniref50_id": "UniRef50_P47351", "cog_id": "COG1624", "cog_category": "T", "kegg_orthology_id": "K18672", "go_ids": [ "GO:0005886", "GO:0004016", "GO:0005524", "GO:0106408", "GO:0006171" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47351", "EC:2.7.7.85", "UniRef:UniRef50_P47351", "COG:COG1624", "COG:T", "KEGG:K18672", "GO:0005886", "GO:0004016", "GO:0005524", "GO:0106408", "GO:0006171" ] }, "pscc": { "uniref50_id": "UniRef50_P47351", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47351" ], "product": "Diadenylate cyclase" } }, "id": "KMADJIBOEG_288", "locus": "GCA000292445_00249" }, { "type": "cds", "sequence": "contig_1", "start": 134483, "stop": 134695, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01327.26" ], "nt": "ATGACTTTTCAACCAACTAAAACTTGGCTTGTTTTTGATGATAATGCTTTGATTAACAAGCCAACTGAAGCTGTTAATTTTCCAATAGATGAGCAAATTGAAACCTGTATTAAAAAGATGATTGCATATGTTGATGCTTCATATGATGGTAAAGCACAAGAATATGACATTATTCCAGGAATTGGGATAGCTGCTAACCAGATTGGCTATTGA", "aa": "MTFQPTKTWLVFDDNALINKPTEAVNFPIDEQIETCIKKMIAYVDASYDGKAQEYDIIPGIGIAANQIGY", "aa_hexdigest": "f68adf1a996fd0d66b84e5bf1b67bbdd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01327.26", "name": "Pep_deformylase", "length": 61, "aa_cov": 0.8571428571428571, "hmm_cov": 0.8032786885245902, "evalue": 2.1339831625382832e-07, "score": 25.789291381835938, "start": 11, "stop": 70 } ], "seq_stats": { "molecular_weight": 7842.8357000000005, "isoelectric_point": 4.2393590927124025 }, "id": "KMADJIBOEG_289", "locus": "GCA000292445_00250" }, { "type": "cds", "sequence": "contig_1", "start": 134736, "stop": 135305, "strand": "+", "frame": 3, "gene": "gmk", "product": "guanylate kinase", "db_xrefs": [ "COG:COG0194", "COG:F", "EC:2.7.4.8", "GO:0004385", "GO:0005524", "GO:0005829", "GO:0006163", "SO:0001217", "UniRef:UniRef50_P47353", "UniRef:UniRef90_P47353" ], "nt": "GTGAACAATCAGGGGAGAATTTTTGTCATTACTGGTCCTAGCGGTGTTGGCAAAAGCACCCTTGTTAAAGCCTTATTAGATCATTTCAAAGAACAACTGTTCTACAGTATCTCTGCAACTACAAGAAAAAAGCGCATTAGTGAAAAAGAGGGAATTGATTATTTTTTTAAAGATAAAGATGAGTTTGAAAACTTAATAAAACAAGATGCTTTCATTGAATGGGCTTGCTATAATAACCATTATTATGGAACGCTCAAGTCTCAAGCTGAACAAGCAATTAAAAGCGGAATTAATTTAATGCTTGAAATTGAGTATCAAGGTGCTTTACAGGTTAAAAGTAAATATCCTCATAACGTTGTTTTAATTTTCATTAAACCACCTTCAATGCAAGAGTTGTTAAAACGTTTAAAAAAGCGTAATGATGAAGATGAAACCACAATTAAAAAACGTTTAGAACAAGCTAAGATAGAGTTTCAACAGATTGATAATTTTAAGTATGTTGTCACTAACAAAGAGTTTGATAAAACCCTTAATGAGTTGAAATCAATCTTACTATCTGAGTTTATTTAA", "aa": "MNNQGRIFVITGPSGVGKSTLVKALLDHFKEQLFYSISATTRKKRISEKEGIDYFFKDKDEFENLIKQDAFIEWACYNNHYYGTLKSQAEQAIKSGINLMLEIEYQGALQVKSKYPHNVVLIFIKPPSMQELLKRLKKRNDEDETTIKKRLEQAKIEFQQIDNFKYVVTNKEFDKTLNELKSILLSEFI", "aa_hexdigest": "95bd95101c47f3b98b91effa28e4a3b9", "start_type": "GTG", "rbs_motif": null, "genes": [ "gmk" ], "truncated": "5-prime", "pseudogene": { "start": 134583, "stop": 135305, "observations": { "mutation": [ 134685 ], "stop": [ 134685 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47353", "query_cov": 1.0, "subject_cov": 0.7875, "identity": 0.995, "score": 367.0, "evalue": 1.71e-127, "gene_start": 1, "gene_end": 189, "reference_start": 52, "reference_end": 240, "reference_sequence": "MIQVSICSSIGKLTASVGLLIKALSSKTSQVLVGWKVIDKNSKVKIILTIEVNNQGRIFVITGPSGVGKSTLVKALLDHFKEQLFYSISATTRKKRISEKEGIDYFFKDKDEFENLIKQDAFIEWACYNNHYYGTLKSQAEQAIKSGINLMLEIEYQGALQVKSKYPHNVVLIFIKPPSMQELLKRLKKRNDEDETTIKKRLEQAKIEFQQIDNFKYVVTNKEFDKTLNELKSILLSEFI" }, "paralog": false, "identity": 0.9916666666666667, "score": 461.0, "evalue": 2.56e-167, "description": "Internal stop codon at 134685. Nonsense mutation around 134685.", "psc": { "uniref90_id": "UniRef90_P47353", "gene": "gmk", "product": "guanylate kinase", "ec_ids": [ "2.7.4.8" ], "uniref50_id": "UniRef50_P47353", "cog_id": "COG0194", "cog_category": "F", "go_ids": [ "GO:0004385", "GO:0005524", "GO:0005829", "GO:0006163" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47353", "EC:2.7.4.8", "UniRef:UniRef50_P47353", "COG:COG0194", "COG:F", "GO:0004385", "GO:0005524", "GO:0005829", "GO:0006163" ] }, "pscc": { "uniref50_id": "UniRef50_P47353", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47353" ], "product": "Guanylate kinase" } }, "id": "KMADJIBOEG_290", "locus": "GCA000292445_00251" }, { "type": "cds", "sequence": "contig_1", "start": 135305, "stop": 135520, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGCTGCTAATGATCATTTCTTTACCGGTTTATCAAAAAAAGGTCCTGTAAGAAAGGAAAATCAGGACTTTTATGGATTTAGTTTTAATCAAAATAACCTACTAATAGTTGTTTGTGATGGTCTTGGTGGTTATAAGGGTGGCAAGATTGCTAGTAACTTAGTAGGTAAACTCTTTCTTAGTTTATTTGAAGGTTTTGAATTTAACCAGTGA", "aa": "MQAANDHFFTGLSKKGPVRKENQDFYGFSFNQNNLLIVVCDGLGGYKGGKIASNLVGKLFLSLFEGFEFNQ", "aa_hexdigest": "9d811b60877e9a5d207cc794be7488c2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7867.837799999999, "isoelectric_point": 7.879471015930176 }, "id": "KMADJIBOEG_291", "locus": "GCA000292445_00252" }, { "type": "cds", "sequence": "contig_1", "start": 135773, "stop": 136087, "strand": "+", "frame": 2, "gene": "pTC1", "product": "Putative protein phosphatase", "db_xrefs": [ "COG:COG0631", "COG:T", "EC:3.1.3.16", "GO:0007165", "GO:0017018", "GO:0033192", "GO:0140791", "GO:0180004", "GO:0180005", "GO:0180006", "GO:0180007", "GO:0180008", "GO:1990439", "KEGG:K20074", "SO:0001217", "UniRef:UniRef50_P47354", "UniRef:UniRef90_P47354" ], "nt": "ATGAATGCTGATGAGAAATTACTTTTAAGTTATTCTAATCAACTTTTAGCATTAACAAATACTATTTCCAAAGAAACAAAAAGACCATTAGTTTATGGTTTTTATAACACAAAAATTGAACAACAAGAATTTTTATTATTGTGTTCTGATGGACTTTATAACTTTGTTGAAAAAGAGCTTTTTTTTGAAATTATCACTAATTCTAAAAACCTAAAACAAGCAGTGTTTAATCTTTATAGAAAAAGTATTGAAAATGCAAGTAATGATAATATTACTGCTGCCCTTGTTAATCTGCAAAAATGGAAGCAATCTTAA", "aa": "MNADEKLLLSYSNQLLALTNTISKETKRPLVYGFYNTKIEQQEFLLLCSDGLYNFVEKELFFEIITNSKNLKQAVFNLYRKSIENASNDNITAALVNLQKWKQS", "aa_hexdigest": "bb93c639f64d7b55e5df02060884917c", "start_type": "ATG", "rbs_motif": "TTATA", "genes": [ "pTC1" ], "truncated": "5-prime", "pseudogene": { "start": 135473, "stop": 136087, "observations": { "mutation": [ 135518, 135683 ], "stop": [ 135518, 135683 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47354", "query_cov": 1.0, "subject_cov": 0.4, "identity": 1.0, "score": 204.0, "evalue": 1.15e-64, "gene_start": 1, "gene_end": 104, "reference_start": 157, "reference_end": 260, "reference_sequence": "MQAANDHFFTGLSKKGPVRKENQDFYGFSFNQNNLLIVVCDGLGGYKGGKIASNLVGKLFLSLFEGFEFNQWDETTVKKWFENTLIQARFQLENCFQTVYEAQIQFARMASTLVLGILTKSDIYIFWIGDSRAYLLFENQAKLVTKDHNLYNQLVAMNADEKLLLSYSNQLLALTNTISKETKRPLVYGFYNTKIEQQEFLLLCSDGLYNFVEKELFFEIITNSKNLKQAVFNLYRKSIENASNDNITAALVNLQKWKQS" }, "paralog": false, "identity": 0.9901960784313726, "score": 396.0, "evalue": 1.53e-142, "description": "Internal stop codon at 135518, 135683. Nonsense mutation around 135518, 135683.", "psc": { "uniref90_id": "UniRef90_P47354", "gene": "PTC1", "product": "Putative protein phosphatase", "ec_ids": [ "3.1.3.16" ], "uniref50_id": "UniRef50_P47354", "cog_id": "COG0631", "cog_category": "T", "kegg_orthology_id": "K20074", "go_ids": [ "GO:0033192", "GO:0140791", "GO:1990439", "GO:0017018", "GO:0180006", "GO:0180007", "GO:0180008", "GO:0180005", "GO:0180004", "GO:0007165" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47354", "EC:3.1.3.16", "UniRef:UniRef50_P47354", "COG:COG0631", "COG:T", "KEGG:K20074", "GO:0033192", "GO:0140791", "GO:1990439", "GO:0017018", "GO:0180006", "GO:0180007", "GO:0180008", "GO:0180005", "GO:0180004", "GO:0007165" ] }, "pscc": { "uniref50_id": "UniRef50_P47354", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47354" ], "product": "Putative protein phosphatase" } }, "id": "KMADJIBOEG_292", "locus": "GCA000292445_00253" }, { "type": "cds", "sequence": "contig_1", "start": 136072, "stop": 136995, "strand": "+", "frame": 1, "gene": "sPS1", "product": "Putative serine/threonine-protein kinase", "db_xrefs": [ "COG:COG0515", "COG:T", "EC:2.7.11.1", "GO:0004674", "GO:0004676", "GO:0004677", "GO:0004679", "GO:0004711", "GO:0005524", "GO:0005886", "GO:0007165", "GO:0035175", "GO:0035402", "GO:0035403", "GO:0035979", "GO:0044022", "GO:0044023", "GO:0044024", "GO:0044025", "GO:0072354", "GO:0072371", "GO:0072518", "GO:0106310", "GO:0140823", "GO:0140855", "GO:0140857", "GO:1990244", "SO:0001217", "UniRef:UniRef50_P47355", "UniRef:UniRef90_P47355" ], "nt": "ATGGAAGCAATCTTAAAGATAGGTGATATTGTTGAAAATAAGTATCAAATTGAAAAGCTTTTAAATAGGGGTGGGATGGATTCCTATCTTTTTTTAGCAAAAAATTTGAACTTAAAAAACTATGGACCAGTTCAAAAAAAACAGTATGGACACTTAGTTTTAAAGGTGGTTCAAAAAAATCCTAAGATTAATGAAAATAATTGGAAAAAGTTCCTTGATGAAATGGTAACCACAACTAGAGTGCACCATTCTAACCTAGTGAAAAGTTTTGATGTGGTGAACCCTTTTTTGAAAATAGTTCGTGGTAATAAAACAATTGCTCTCAATCAGATAGTGATGATTGCAATGGAGTATGTTGATGGCCCTTCTTTAAGACAATTGCTTAACAGAAAAGGCTACTTTAGTGTTAGCGAAGTAGTTTATTACTTTACAAAGATAGTAAAAGCCATTGATTATCTCCACAGTTTCAAGCACCAAATTATCCACCGTGATCTTAAACCTGAAAACATCTTATTTACTAGTGATTTAACTGATATTAAACTATTAGATTTTGGCATTGCTTCAACAGTAGTTAAAGTTGCTGAAAAAACCGAAGTTTTAACTGATGAAAATTCACTGTTTGGCACAGTTAGTTATATGATTCCTGATGTTTTGGAAAGCACAGTTAATAAAGCTGGTAAAAAGGTTAGAAAACCACCCAATGCCCAGTATGATATCTACTCGTTAGGAATTATTCTATTTGAGATGTTAGTTGGTAGAGTTCCATTTAACAAGTCAATTAACCCCAACAAGGAAAGAGAAACAATCCAAAAAGCACGCAATTTTGACTTACCTTTAATGCAAGCAACTAGAAGTGATATCCCAAATAGCTTAGAAAATATTGCTTTTCGTTGTACTGCTGTGAAAAGAGAAAACAATAAGTGA", "aa": "MEAILKIGDIVENKYQIEKLLNRGGMDSYLFLAKNLNLKNYGPVQKKQYGHLVLKVVQKNPKINENNWKKFLDEMVTTTRVHHSNLVKSFDVVNPFLKIVRGNKTIALNQIVMIAMEYVDGPSLRQLLNRKGYFSVSEVVYYFTKIVKAIDYLHSFKHQIIHRDLKPENILFTSDLTDIKLLDFGIASTVVKVAEKTEVLTDENSLFGTVSYMIPDVLESTVNKAGKKVRKPPNAQYDIYSLGIILFEMLVGRVPFNKSINPNKERETIQKARNFDLPLMQATRSDIPNSLENIAFRCTAVKRENNK", "aa_hexdigest": "3ae8cd8ba63e45f2ed275564bb089a3d", "start_type": "ATG", "rbs_motif": null, "genes": [ "sPS1" ], "truncated": "3-prime", "pseudogene": { "start": 136072, "stop": 137235, "observations": { "mutation": [ 136993, 137038, 137149 ], "stop": [ 136993, 137038, 137149 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47355", "query_cov": 1.0, "subject_cov": 0.7932816537467701, "identity": 1.0, "score": 602.0, "evalue": 4.8e-216, "gene_start": 1, "gene_end": 307, "reference_start": 1, "reference_end": 307, "reference_sequence": "MEAILKIGDIVENKYQIEKLLNRGGMDSYLFLAKNLNLKNYGPVQKKQYGHLVLKVVQKNPKINENNWKKFLDEMVTTTRVHHSNLVKSFDVVNPFLKIVRGNKTIALNQIVMIAMEYVDGPSLRQLLNRKGYFSVSEVVYYFTKIVKAIDYLHSFKHQIIHRDLKPENILFTSDLTDIKLLDFGIASTVVKVAEKTEVLTDENSLFGTVSYMIPDVLESTVNKAGKKVRKPPNAQYDIYSLGIILFEMLVGRVPFNKSINPNKERETIQKARNFDLPLMQATRSDIPNSLENIAFRCTAVKRENNKWLYSSTKELLEDLANWENEQAMIKPANERVLEGQVEIREMMLEKPLAWYFKTWALSIFTIVFIGLIIAAIVLLLIFNARF" }, "paralog": false, "identity": 0.9922480620155039, "score": 748.0, "evalue": 4.35e-276, "description": "Internal stop codon at 136993, 137038, 137149. Nonsense mutation around 136993, 137038, 137149.", "psc": { "uniref90_id": "UniRef90_P47355", "gene": "SPS1", "product": "Putative serine/threonine-protein kinase", "ec_ids": [ "2.7.11.1" ], "uniref50_id": "UniRef50_P47355", "cog_id": "COG0515", "cog_category": "T", "go_ids": [ "GO:0005886", "GO:0004676", "GO:0004679", "GO:0005524", "GO:0004677", "GO:0044024", "GO:0072371", "GO:1990244", "GO:0035979", "GO:0044025", "GO:0140823", "GO:0035175", "GO:0044022", "GO:0140855", "GO:0035402", "GO:0072354", "GO:0140857", "GO:0035403", "GO:0044023", "GO:0106310", "GO:0004674", "GO:0072518", "GO:0004711", "GO:0007165" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47355", "EC:2.7.11.1", "UniRef:UniRef50_P47355", "COG:COG0515", "COG:T", "GO:0005886", "GO:0004676", "GO:0004679", "GO:0005524", "GO:0004677", "GO:0044024", "GO:0072371", "GO:1990244", "GO:0035979", "GO:0044025", "GO:0140823", "GO:0035175", "GO:0044022", "GO:0140855", "GO:0035402", "GO:0072354", "GO:0140857", "GO:0035403", "GO:0044023", "GO:0106310", "GO:0004674", "GO:0072518", "GO:0004711", "GO:0007165" ] }, "pscc": { "uniref50_id": "UniRef50_P47355", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47355" ], "product": "Putative serine/threonine-protein kinase" } }, "id": "KMADJIBOEG_293", "locus": "GCA000292445_00254" }, { "type": "cds", "sequence": "contig_1", "start": 137056, "stop": 137151, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAAACCTGCTAATGAAAGGGTTTTAGAAGGGCAGGTGGAAATTAGAGAAATGATGCTTGAAAAGCCCTTAGCTTGGTATTTCAAAACTTGA", "aa": "MIKPANERVLEGQVEIREMMLEKPLAWYFKT", "aa_hexdigest": "c33c19896e15df6b6658bc9374a8b804", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3751.4417000000003, "isoelectric_point": 6.0999265670776355 }, "id": "KMADJIBOEG_294", "locus": "GCA000292445_00255" }, { "type": "cds", "sequence": "contig_1", "start": 137348, "stop": 137488, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGGTGATAGGGTTCAACTTGAAGATGGTGCGATCACTAAAGTTTTAGCTAGAAAAAATGAACTTACAAGACCAAGGGTTGCTAATGTTGATCAGATTGTTTTAATCCAATCACTAGTTCAACCTAAGATTAACTGA", "aa": "MVGDRVQLEDGAITKVLARKNELTRPRVANVDQIVLIQSLVQPKIN", "aa_hexdigest": "189b228caee193f8e7f40df2b8cfff7b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5139.973, "isoelectric_point": 9.687615776062014 }, "id": "KMADJIBOEG_295", "locus": "GCA000292445_00256" }, { "type": "cds", "sequence": "contig_1", "start": 137498, "stop": 138025, "strand": "+", "frame": 2, "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "db_xrefs": [ "COG:COG1162", "COG:J", "EC:3.6.1.-", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0019843", "GO:0042274", "GO:0043022", "GO:0046872", "KEGG:K06949", "SO:0001217", "UniRef:UniRef50_P75523", "UniRef:UniRef90_P47356" ], "nt": "TTGAAATTATTGGTATATTTCAATGCTAAATTAATTGATGAAATACCTATTTTAATAACAAAAACAGACCTTGATTTTGATCCAATGGAAAAACAAAAGTTAATCGATTTAAAACAGTTTAACTATCAGTTATTTTTTGTTTCTAAAAATGAACCACTACCTTCAGAATTAATCGATATTTTTAGTAAAAAACTAAGTGTTTTTACAGGTCAATCTGGTGTTGGTAAATCTAGCTTAATTAATCGTTTAGATCCTTCTTTAAAACAAAAGATTCAAGCCTTATCAGTTAATAAATTTGGTAAGAATACCACTACTAAAACAACACTTTTTTCATTTAGAGGCGGTTTTATTTGTGACACCCCTGGTTTTAATGTAATTTCTATTAAAAACCTCAAAATTTTAGCAGCCCAACACTTTGTTGGTTTTCAGAAAATGATTAGTAAGTGTCATTTTTCTAACTGTTATCATCAGTATGAAAAAGATTGCTTTGTAACCACAAGTGTTATGAAAAACAGATATCCTTCGTGA", "aa": "MKLLVYFNAKLIDEIPILITKTDLDFDPMEKQKLIDLKQFNYQLFFVSKNEPLPSELIDIFSKKLSVFTGQSGVGKSSLINRLDPSLKQKIQALSVNKFGKNTTTKTTLFSFRGGFICDTPGFNVISIKNLKILAAQHFVGFQKMISKCHFSNCYHQYEKDCFVTTSVMKNRYPS", "aa_hexdigest": "840f77a830b4db4270930cab4d57a428", "start_type": "TTG", "rbs_motif": null, "genes": [ "rsgA" ], "truncated": "both", "pseudogene": { "start": 137222, "stop": 138058, "observations": { "mutation": [ 137327, 137486, 138023 ], "stop": [ 137327, 137486, 138023 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47356", "query_cov": 1.0, "subject_cov": 0.6294964028776978, "identity": 0.9940000000000001, "score": 348.0, "evalue": 1.06e-119, "gene_start": 1, "gene_end": 175, "reference_start": 93, "reference_end": 267, "reference_sequence": "MPDSNFGIVLQSLAKQCKVFWNNQIITAFPQKKLQWKNDFKLMVGDRVQLEDGAITKVLARKNELTRPRVANVDQIVLIQSLVQPKINWIQLLKLLVYFNAKLIDEIPILITKTDLDFDPMEKQKLIDLKQFNYQLFFVSKNEPLPSELIDIFSKKLSVFTGQSGVGKSSLINRLDPSLKQKIQALSVNKFGKNTTTKTTLFSFRGGFICDTPGFNVISIKNLKILAAQHFVGFQKMISKCHFSNCYHQYEKDCFVTTSVMKNRYPSWLYEKYRKMIN" }, "paralog": false, "identity": 0.9892086330935251, "score": 543.0, "evalue": 3.22e-199, "description": "Internal stop codon at 137327, 137486, 138023. Nonsense mutation around 137327, 137486, 138023.", "psc": { "uniref90_id": "UniRef90_P47356", "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "ec_ids": [ "3.6.1.-" ], "uniref50_id": "UniRef50_P75523", "cog_id": "COG1162", "cog_category": "J", "kegg_orthology_id": "K06949", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0019843", "GO:0042274", "GO:0043022", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47356", "EC:3.6.1.-", "UniRef:UniRef50_P75523", "COG:COG1162", "COG:J", "KEGG:K06949", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0019843", "GO:0042274", "GO:0043022", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P75523", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75523" ], "product": "Small ribosomal subunit biogenesis GTPase RsgA" } }, "id": "KMADJIBOEG_296", "locus": "GCA000292445_00257" }, { "type": "cds", "sequence": "contig_1", "start": 138079, "stop": 138834, "strand": "+", "frame": 1, "gene": "pgi", "product": "glucose-6-phosphate isomerase", "db_xrefs": [ "COG:COG0166", "COG:G", "EC:5.3.1.9", "GO:0004347", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0048029", "GO:0051156", "GO:0097367", "SO:0001217", "UniRef:UniRef50_P47357", "UniRef:UniRef90_P47357" ], "nt": "ATGAGTGATAAATTATTAACAATTGACTTAAGTCATGTTTATGGATTTGATAAAGAAATTATTTTTAAGAAATACCAAAAAAAAGTAGATCAAATTCACCAAGATTTTCTAGCTCATAAACTTGCTGATGGTCACATGACTGGGTGGTATGACCAACCTGATCAAAACCACCAATTCCTTTTAAAAACCATTAATCAAATTGACAAAAAGTTTAAAAGTTTAAAAGTAACTGACATTGTTTATGTTGGTATTGGTGGTTCTTTTACTGGTATTAAAACAGTTTTAGATTTCTTAAAACCAAAACAAAGAACAGGATTAAAAATCCACTTTGTCCCTGACCTTTCTGCTTTTCAAGCTGCAAGTGTTATTAAGGAAATTAAAAATAAATCATGGGCTCTAATTACCACTTCTAAGTCTGGTAGAACCCTAGAACCAGCACTGAATTTCCGCATTTTTAGAAACTTATTAAACAAGCGTTATGGCAACAAACACTACCAAAGAGTAGTTGTTATTACTGATGAAAAAAAGGGATTACTAACCAAAATGGCATCAAATCATGGTTACCAAAAGTTAGTTATTGATTCAAATATCGGTGGGCGTTTTTCAACTCTATCTCCTGCTGGTTTGTTACTAGCCAAACTTTTTGGTCATGATCCTAAGGCCATCTTAAAAGGAACATTACAAGCCAAAAAGGATTTGCAAACAACTTCACTTGAAAACAATTCTGCATACCTTTATGCAGTAGTTAGACATTGA", "aa": "MSDKLLTIDLSHVYGFDKEIIFKKYQKKVDQIHQDFLAHKLADGHMTGWYDQPDQNHQFLLKTINQIDKKFKSLKVTDIVYVGIGGSFTGIKTVLDFLKPKQRTGLKIHFVPDLSAFQAASVIKEIKNKSWALITTSKSGRTLEPALNFRIFRNLLNKRYGNKHYQRVVVITDEKKGLLTKMASNHGYQKLVIDSNIGGRFSTLSPAGLLLAKLFGHDPKAILKGTLQAKKDLQTTSLENNSAYLYAVVRH", "aa_hexdigest": "07eef0244e1c0be1a3f0164ea79da9dd", "start_type": "ATG", "rbs_motif": null, "genes": [ "pgi" ], "truncated": "3-prime", "pseudogene": { "start": 138079, "stop": 139137, "observations": { "mutation": [ 138832 ], "stop": [ 138832 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47357", "query_cov": 1.0, "subject_cov": 0.5823665893271461, "identity": 1.0, "score": 501.0, "evalue": 9.23e-177, "gene_start": 1, "gene_end": 251, "reference_start": 1, "reference_end": 251, "reference_sequence": "MSDKLLTIDLSHVYGFDKEIIFKKYQKKVDQIHQDFLAHKLADGHMTGWYDQPDQNHQFLLKTINQIDKKFKSLKVTDIVYVGIGGSFTGIKTVLDFLKPKQRTGLKIHFVPDLSAFQAASVIKEIKNKSWALITTSKSGRTLEPALNFRIFRNLLNKRYGNKHYQRVVVITDEKKGLLTKMASNHGYQKLVIDSNIGGRFSTLSPAGLLLAKLFGHDPKAILKGTLQAKKDLQTTSLENNSAYLYAVVRHWLYTTKKFKIEVCIAYHSLYEYLLLQHRQLFGESEGKNDKSLFPTFSIFTVDLHSMGQLYQEGEKVFFETVIDVKNPLVNINLPPSDFDNDDELDFLLDKSLNEISDVAIDSVIKAHYQANVSIIKLTLKEQSAFMFGYFYFWLSVATVMSGSLLGHNVFNQPGVEVYKKLMFEKLRSGH" }, "paralog": false, "identity": 0.9971590909090909, "score": 703.0, "evalue": 1.17e-258, "description": "Internal stop codon at 138832. Nonsense mutation around 138832.", "psc": { "uniref90_id": "UniRef90_P47357", "gene": "pgi", "product": "glucose-6-phosphate isomerase", "ec_ids": [ "5.3.1.9" ], "uniref50_id": "UniRef50_P47357", "cog_id": "COG0166", "cog_category": "G", "go_ids": [ "GO:0004347", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0048029", "GO:0051156", "GO:0097367" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47357", "EC:5.3.1.9", "UniRef:UniRef50_P47357", "COG:COG0166", "COG:G", "GO:0004347", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0048029", "GO:0051156", "GO:0097367" ] }, "pscc": { "uniref50_id": "UniRef50_P47357", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47357" ], "product": "Glucose-6-phosphate isomerase" } }, "id": "KMADJIBOEG_297", "locus": "GCA000292445_00258" }, { "type": "cds", "sequence": "contig_1", "start": 138886, "stop": 139260, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATGAATATTTGTTATTACAGCATCGACAACTTTTTGGTGAATCAGAAGGTAAGAACGATAAATCTTTATTTCCTACTTTTTCGATTTTTACTGTTGACTTACACTCAATGGGACAACTCTATCAAGAAGGGGAAAAAGTGTTTTTTGAAACAGTAATTGATGTTAAAAATCCACTTGTTAATATTAATTTACCTCCATCTGATTTTGACAATGATGATGAACTTGATTTCTTGTTAGATAAAAGCTTAAATGAGATTTCAGATGTTGCAATTGATTCAGTTATTAAAGCGCACTACCAAGCAAATGTAAGCATTATTAAATTAACTTTAAAAGAACAATCTGCATTTATGTTTGGTTATTTTTACTTTTGA", "aa": "MYEYLLLQHRQLFGESEGKNDKSLFPTFSIFTVDLHSMGQLYQEGEKVFFETVIDVKNPLVNINLPPSDFDNDDELDFLLDKSLNEISDVAIDSVIKAHYQANVSIIKLTLKEQSAFMFGYFYF", "aa_hexdigest": "9c21bc0eb807d4b4ad1c0a13b1c0f4ec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14374.081199999979, "isoelectric_point": 4.418231391906739 }, "id": "KMADJIBOEG_298", "locus": "GCA000292445_00259" }, { "type": "cds", "sequence": "contig_1", "start": 139364, "stop": 139993, "strand": "+", "frame": 2, "gene": "rpe", "product": "Ribulose-phosphate 3-epimerase", "db_xrefs": [ "COG:COG0036", "COG:G", "EC:5.1.3.1", "GO:0004750", "GO:0005829", "GO:0005975", "GO:0009052", "GO:0019323", "GO:0046872", "RefSeq:WP_009885669.1", "SO:0001217", "UniParc:UPI0000134621", "UniRef:UniRef100_P47358", "UniRef:UniRef50_P47358", "UniRef:UniRef90_P47358" ], "nt": "GTGGCCACTAAGGTTGTTTTTTCACTCTTACCACTTTTAAATAGGTTTGACAAGTCACTTTTAGAAAGTTACTTTCAAGATGGATTGAGGTTAATCCATTATGATGTGATGGACCAATTTGTTCATAATACTGCTTTTAAAGGTGAATATTTGGATGAATTGAAAACAATAGGTTTTGATGTTAATGTCCATTTAATGGTGGAACAGATCATCCCTCAAATAAATTTTTATCTTTCACAACCTAATGTGAAAAGGATTTCGTTTCATGTTGAACCATTTAGTTTTGCAAAGATTAAAGAACTAATCCAACTAGTTAAAGAAAATGGTAAAGAAGTTGGTCTTGCTTTTAAATTTACAACCAATTTACAACTATACCAACCATTTTTTACAACCATCGACTTTATCACTTTAATGAGTGTTCCTCCTGGTAAAGGTGGTCAAGCTTTTAACGAAGCTGTTTTTACAAATTTAAAGATTGCTAACCATTACAACTTGAAAATTGAGATTGATGGTGGGATTAAAGTTAATAACATTGATCAAATTAAAGCCTTTGTTGATTTCATTGTAATGGGAAGTGGCTTTATAAAATTAGAGCAGTGGCAACGTCAAAAATTGTTGCAAACAATCTAA", "aa": "MATKVVFSLLPLLNRFDKSLLESYFQDGLRLIHYDVMDQFVHNTAFKGEYLDELKTIGFDVNVHLMVEQIIPQINFYLSQPNVKRISFHVEPFSFAKIKELIQLVKENGKEVGLAFKFTTNLQLYQPFFTTIDFITLMSVPPGKGGQAFNEAVFTNLKIANHYNLKIEIDGGIKVNNIDQIKAFVDFIVMGSGFIKLEQWQRQKLLQTI", "aa_hexdigest": "3e9c16fedfdc638449e854ff97b054d3", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134621", "ncbi_nrp_id": "WP_009885669.1", "uniref100_id": "UniRef100_P47358", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134621", "RefSeq:WP_009885669.1", "UniRef:UniRef100_P47358" ] }, "ips": { "uniref100_id": "UniRef100_P47358", "uniref90_id": "UniRef90_P47358" }, "psc": { "uniref90_id": "UniRef90_P47358", "gene": "rpe", "product": "Ribulose-phosphate 3-epimerase", "ec_ids": [ "5.1.3.1" ], "uniref50_id": "UniRef50_P47358", "cog_id": "COG0036", "cog_category": "G", "go_ids": [ "GO:0005829", "GO:0004750", "GO:0046872", "GO:0005975", "GO:0019323", "GO:0009052" ] }, "pscc": { "uniref50_id": "UniRef50_P47358", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47358" ], "product": "Ribulose-phosphate 3-epimerase" }, "genes": [ "rpe" ], "id": "KMADJIBOEG_299", "locus": "GCA000292445_00260" }, { "type": "cds", "sequence": "contig_1", "start": 140007, "stop": 140240, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01336.31" ], "nt": "ATGAAATCAGTTACAGTCAAGCAATTACTACAAACCCCACGAAAATTTAATAACAAGCAGATTAAACTATCAGGTTGGGTTAAAAATAAACGTGCTAGTGCTAACATCATCTTTCTAGCAATTAGTGATGGCTCTAGTATTAATACCCTACAAGCAGTAGTAAAACAAGAAGATAACCCCCAGGTTTTCTCACTGTTACAAACTGTTAATTTAACAAGTGCTGTTATGGTTTGA", "aa": "MKSVTVKQLLQTPRKFNNKQIKLSGWVKNKRASANIIFLAISDGSSINTLQAVVKQEDNPQVFSLLQTVNLTSAVMV", "aa_hexdigest": "f5c25dba83306335b5f5650bc862360b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01336.31", "name": "tRNA_anti-codon", "length": 47, "aa_cov": 0.5974025974025974, "hmm_cov": 0.9574468085106383, "evalue": 4.8646619712409177e-08, "score": 28.026779174804688, "start": 22, "stop": 67 } ], "seq_stats": { "molecular_weight": 8529.888, "isoelectric_point": 10.461107444763183 }, "id": "KMADJIBOEG_300", "locus": "GCA000292445_00261" }, { "type": "cds", "sequence": "contig_1", "start": 140298, "stop": 141233, "strand": "+", "frame": 3, "gene": "asnS", "product": "asparagine--tRNA ligase", "db_xrefs": [ "COG:COG0017", "COG:J", "EC:6.1.1.22", "GO:0000166", "GO:0003676", "GO:0004816", "GO:0005524", "GO:0005737", "GO:0006421", "KEGG:K01893", "SO:0001217", "UniRef:UniRef50_P47359", "UniRef:UniRef90_P47359" ], "nt": "GTGAGTTTATTAGCACAAGCAGAGTCTGATTATCCACTGCAAAAAAAAGAACATAGTCAAGAGTTTTTTAGAAGTAATGCGCATCTAAGAGTAAGAGCAAAAACTTACTTTGCAGTGATGAAAATAAGGAGTGTTTTGTCACACGCAATCTTTGAATACTTCTTTAAAAATGATTTTATCTTAGTGCAAAGCCCTATTTTAACTAGTAATGATTGTGAGGGAGCGGGGGAAACATTTGTAATTAAAGATAGTGAAACTTTTTTTAATAAAACGACTTTTTTAACAGTAAGTGGCCAGTTTGGAGCAGAAGCTTTTGCGCAAGCATTTAAAAAGGTTTTCACCTTTGGTCCTACTTTCAGAGCTGAAAAATCCCATACTAATCGTCATCTTAGTGAGTTTTGGATGATCGAACCTGAAATTGCATTTGCTAACTTAAAAGATTTAATGCAGTTAATACAAAACCTAATTAAATTCTTAATTAAAAAAGTGATGGAAAATGCTAGTGATGAACTAAATGTTTTAGCAAAGCAATTTAGCAATGACATTATTAGCAACTTAAAGACAATCATTAGTACTAAAAAATTTCCAATCATTGAATACAGCAAAGCATTAGCGATTCTAAAGGAATCTAGTGATACAAAAAAAACTAATTTTGAACTAAACGACTTTAGTTTTGGTATTGACTTAAAAACAGAACATGAACGCTTTTTGTGCGAACAATATTTTCAAAATCAACCGCTTTTTGTTATTAACTATCCAAAGGAGTTAAAGGCATTTTACATGAAAACAAATACTGACAATAAAACTGTTGCTGCAGTTGATCTTTTATTACCAAAGATTGGTGAGATTTGTGGGGGAAGTGAAAGGGAAAGTGATTTAAACCAACTTAAGAATAGGTGTCAATCTTTAAACATTGACACAAAAAGTTTGAACTGA", "aa": "MSLLAQAESDYPLQKKEHSQEFFRSNAHLRVRAKTYFAVMKIRSVLSHAIFEYFFKNDFILVQSPILTSNDCEGAGETFVIKDSETFFNKTTFLTVSGQFGAEAFAQAFKKVFTFGPTFRAEKSHTNRHLSEFWMIEPEIAFANLKDLMQLIQNLIKFLIKKVMENASDELNVLAKQFSNDIISNLKTIISTKKFPIIEYSKALAILKESSDTKKTNFELNDFSFGIDLKTEHERFLCEQYFQNQPLFVINYPKELKAFYMKTNTDNKTVAAVDLLLPKIGEICGGSERESDLNQLKNRCQSLNIDTKSLN", "aa_hexdigest": "c8604be61c724bce98994c13cdde93fc", "start_type": "GTG", "rbs_motif": null, "genes": [ "asnS" ], "truncated": "both", "pseudogene": { "start": 140007, "stop": 141377, "observations": { "mutation": [ 140238, 141231 ], "stop": [ 140238, 141231 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47359", "query_cov": 1.0, "subject_cov": 0.6820175438596491, "identity": 0.997, "score": 603.0, "evalue": 1.2e-215, "gene_start": 1, "gene_end": 311, "reference_start": 98, "reference_end": 408, "reference_sequence": "MKSVTVKQLLQTPRKFNNKQIKLSGWVKNKRASANIIFLAISDGSSINTLQAVVKQEDNPQVFSLLQTVNLASAVMVWGEIILTPKAKQPLELKLKQVSLLAQAESDYPLQKKEHSQEFFRSNAHLRVRAKTYFAVMKIRSVLSHAIFEYFFKNDFILVQSPILTSNDCEGAGETFVIKDSETFFNKTTFLTVSGQFGAEAFAQAFKKVFTFGPTFRAEKSHTNRHLSEFWMIEPEIAFANLKDLMQLIQNLIKFLIKKVMENASDELNVLAKQFSNDIISNLKTIISTKKFPIIEYSKALAILKESSDTKKTNFELNDFSFGIDLKTEHERFLCEQYFQNQPLFVINYPKELKAFYMKTNTDNKTVAAVDLLLPKIGEICGGSERESDLNQLKNRCQSLNIDTKSLNWYLDMRKWGYFASAGFGLGFDRLLAYICGLENIRDAIPFPRVHGTINF" }, "paralog": false, "identity": 0.993421052631579, "score": 890.0, "evalue": 0.0, "description": "Internal stop codon at 140238, 141231. Nonsense mutation around 140238, 141231.", "psc": { "uniref90_id": "UniRef90_P47359", "gene": "asnS", "product": "asparagine--tRNA ligase", "ec_ids": [ "6.1.1.22" ], "uniref50_id": "UniRef50_P47359", "cog_id": "COG0017", "cog_category": "J", "kegg_orthology_id": "K01893", "go_ids": [ "GO:0000166", "GO:0003676", "GO:0004816", "GO:0005524", "GO:0005737", "GO:0006421" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47359", "EC:6.1.1.22", "UniRef:UniRef50_P47359", "COG:COG0017", "COG:J", "KEGG:K01893", "GO:0000166", "GO:0003676", "GO:0004816", "GO:0005524", "GO:0005737", "GO:0006421" ] }, "pscc": { "uniref50_id": "UniRef50_P47359", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47359" ], "product": "Asparagine--tRNA ligase" } }, "id": "KMADJIBOEG_301", "locus": "GCA000292445_00262" }, { "type": "cds", "sequence": "contig_1", "start": 141243, "stop": 141377, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGAGGAAATGGGGTTATTTTGCTAGTGCAGGTTTTGGTTTGGGCTTTGATAGATTATTAGCTTATATATGTGGATTGGAAAACATCAGAGATGCTATTCCCTTTCCCCGTGTACATGGCACCATTAACTTCTAA", "aa": "MRKWGYFASAGFGLGFDRLLAYICGLENIRDAIPFPRVHGTINF", "aa_hexdigest": "fe5196cc5a7e35d0d9f2fd7fa4dfda18", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A378BFT2", "query_cov": 1.0, "subject_cov": 0.9166666666666666, "identity": 0.5, "score": 55.5, "evalue": 2.13e-09, "valid": false, "product": "Asparaginyl-tRNA synthetase", "uniref50_id": "UniRef50_A0A378BFT2" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 37, "aa_cov": 0.8409090909090909, "hmm_cov": 1.0, "evalue": 1.903983273755394e-11, "score": 38.68692398071289, "start": 2, "stop": 38 } ], "seq_stats": { "molecular_weight": 4991.7507, "isoelectric_point": 9.183408546447755 }, "id": "KMADJIBOEG_302", "locus": "GCA000292445_00263" }, { "type": "cds", "sequence": "contig_1", "start": 141334, "stop": 141636, "strand": "+", "frame": 1, "gene": "pgsA", "product": "CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase", "db_xrefs": [ "COG:COG0558", "COG:I", "EC:2.7.8.5", "GO:0005886", "GO:0006655", "GO:0008444", "GO:0008654", "GO:0016020", "GO:0046474", "SO:0001217", "UniRef:UniRef50_P47360", "UniRef:UniRef90_P47360" ], "nt": "ATGCTATTCCCTTTCCCCGTGTACATGGCACCATTAACTTCTAAATTCGCTGCTTATAAAAAAAAGATTGCAAACTGGTTAACAGTTTACAGAATTTTTATTGCTTTACCTACTATTATTTTTATTGCTTTAGATAATCAACTAGGAGTTTTAGCTAACTTTTCTGTTGGTGCAATTAGCATTAGTTTACAGATCAGTTTATTGATTGGAGGATTTTTGTTTTTAACTGCAGTTATATCAGATTATTTAGATGGATATTTAGCAAGAAAATGGCTAGCAGTTTCTAACTTTGGTAAATTATGA", "aa": "MLFPFPVYMAPLTSKFAAYKKKIANWLTVYRIFIALPTIIFIALDNQLGVLANFSVGAISISLQISLLIGGFLFLTAVISDYLDGYLARKWLAVSNFGKL", "aa_hexdigest": "fb905faad3c5ae4d0b8c9279a7c59a79", "start_type": "ATG", "rbs_motif": null, "genes": [ "pgsA" ], "truncated": "3-prime", "pseudogene": { "start": 141313, "stop": 141939, "observations": { "mutation": [ 141634, 141799, 141811, 141910 ], "stop": [ 141634, 141799, 141811, 141910 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47360", "query_cov": 1.0, "subject_cov": 0.423728813559322, "identity": 1.0, "score": 191.0, "evalue": 1.35e-59, "gene_start": 1, "gene_end": 100, "reference_start": 8, "reference_end": 107, "reference_sequence": "MDWKTSEMLFPFPVYMAPLTSKFAAYKKKIANWLTVYRIFIALPTIIFIALDNQLGVLANFSVGAISISLQISLLIGGFLFLTAVISDYLDGYLARKWLAVSNFGKLWDPIADKVIINGVLIALAINGYFHFSLLIVFIVRDLVLDGMRIYAYEKKVVIAANWLGKWKTIMQMVGIVFSCFVWSFKQSEIASLNSGLFFWLLTQLPYYLAAVFSIWSFIVYNIQIYQQLKAYNSKL" }, "paralog": false, "identity": 0.9759615384615384, "score": 393.0, "evalue": 9.22e-142, "description": "Internal stop codon at 141634, 141799, 141811, 141910. Nonsense mutation around 141634, 141799, 141811, 141910.", "psc": { "uniref90_id": "UniRef90_P47360", "gene": "pgsA", "product": "CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase", "ec_ids": [ "2.7.8.5" ], "uniref50_id": "UniRef50_P47360", "cog_id": "COG0558", "cog_category": "I", "go_ids": [ "GO:0005886", "GO:0006655", "GO:0008444", "GO:0008654", "GO:0016020", "GO:0046474" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47360", "EC:2.7.8.5", "UniRef:UniRef50_P47360", "COG:COG0558", "COG:I", "GO:0005886", "GO:0006655", "GO:0008444", "GO:0008654", "GO:0016020", "GO:0046474" ] }, "pscc": { "uniref50_id": "UniRef50_P47360", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47360" ], "product": "CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase" } }, "id": "KMADJIBOEG_303", "locus": "GCA000292445_00264" }, { "type": "cds", "sequence": "contig_1", "start": 141655, "stop": 141801, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTATCAATGGTGTTCTTATTGCACTAGCGATTAATGGATATTTTCACTTTAGCTTATTAATTGTTTTTATAGTCCGTGATCTTGTGTTGGATGGAATGCGGATTTATGCTTATGAGAAAAAGGTGGTTATTGCTGCTAACTGA", "aa": "MIINGVLIALAINGYFHFSLLIVFIVRDLVLDGMRIYAYEKKVVIAAN", "aa_hexdigest": "ea3f372f1427cd7cb5a01ce016ab5adc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5411.513300000001, "isoelectric_point": 8.16035976409912 }, "id": "KMADJIBOEG_304", "locus": "GCA000292445_00265" }, { "type": "cds", "sequence": "contig_1", "start": 142048, "stop": 142521, "strand": "+", "frame": 1, "gene": "pncC", "product": "Protein MG115", "db_xrefs": [ "COG:COG1546", "COG:H", "EC:3.5.1.42", "KEGG:K03743", "RefSeq:WP_009885673.1", "SO:0001217", "UniParc:UPI00001392B9", "UniRef:UniRef100_P47361", "UniRef:UniRef50_P47361", "UniRef:UniRef90_P47361" ], "nt": "TTGTTTGCCAATTTAATAGCAGAAAAACTCCAAAAGTTACAACTTAGTGTTGCAACAGCTGAATCAGTTACTGGTGGCTTATTAGCTCATTGTTTAACTTCCATTGATGGTGCTTCTAACTATTTTAATGGCGGTGTTATTGCTTACAATAACCAAGTTAAGATTAACTTACTGAATGTTCAATCCTCCACAATTGCAAACCATGGGGCAGTTTCCAGTTTCTGTGCTAGGGAGATGGCAGTTGGGGTTAAACAAAAGTTTCAAGCTGATGTGGGTATTGCTTGCAGTGGGATAGCAGGTAGTAAAGCAGTTGAAAATAAAGCAATAGGATTACTTTTTTTCTGTATTATTATAGGAAATAAGGCTTATGATTTTGAGTTTGAAATGAACCAAAATAATCGTAAGGATAACATTGAATTATTTACCAATAAGATCTTGGAATCTTTCCACTATTTGTTAACAAAGCTAGCTTAA", "aa": "MFANLIAEKLQKLQLSVATAESVTGGLLAHCLTSIDGASNYFNGGVIAYNNQVKINLLNVQSSTIANHGAVSSFCAREMAVGVKQKFQADVGIACSGIAGSKAVENKAIGLLFFCIIIGNKAYDFEFEMNQNNRKDNIELFTNKILESFHYLLTKLA", "aa_hexdigest": "ee88f60c5e80f1baaaefcce45f207bc5", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001392B9", "ncbi_nrp_id": "WP_009885673.1", "uniref100_id": "UniRef100_P47361", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001392B9", "RefSeq:WP_009885673.1", "UniRef:UniRef100_P47361" ] }, "ips": { "uniref100_id": "UniRef100_P47361", "uniref90_id": "UniRef90_P47361" }, "psc": { "uniref90_id": "UniRef90_P47361", "gene": "PncC", "product": "Protein MG115", "ec_ids": [ "3.5.1.42" ], "uniref50_id": "UniRef50_P47361", "cog_id": "COG1546", "cog_category": "H", "kegg_orthology_id": "K03743" }, "pscc": { "uniref50_id": "UniRef50_P47361", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47361" ], "product": "Protein MG115" }, "genes": [ "pncC" ], "id": "KMADJIBOEG_305", "locus": "GCA000292445_00266" }, { "type": "cds", "sequence": "contig_1", "start": 142530, "stop": 143063, "strand": "+", "frame": 3, "gene": "ispD", "product": "putative protein MG116", "db_xrefs": [ "COG:COG1211", "COG:I", "SO:0001217", "UniRef:UniRef50_P75519", "UniRef:UniRef90_P47362" ], "nt": "ATGAAACGTACTTTAAATATAGGTATTGTTTTGTGTGAAAATTTTCTTTCAGACCAACAAAATGCTGTTGATAGTTACACCCAAGTTTATGAAGATGTTAGGATGTTTGAATTTGGGTTAAAACTCTTTCAATCATTACCTTTTAACATCCAAGAAACCCTTATATTTTGTAATGCTGAACAACATAAAATAGTTGATAAAGCTGCTAAAAAATACAAAAACACTACTGTGTTTTTTTCACGTGATACTGATGTAGCTAATGTTTATGAAGCTAAGGTATTTATCCAAGAAAAATACAAATTAACCCAAGATTACAAAAAAAGAGGTGTTAGCTCTTATTATGACAGCTGTTGCTTTATCTTAATTGAAGCGAATCGTCCATTAACTGCAATAAAGACAGTTAAAAGTGTTTATGAAAAAGCACTGATAGAAAAAGCAGCTATTGCAGTATTACCATATAATGGTACACTAATGAATGGTAACAATGATGTTGTCTTTAGCCACTTGCAAGATAAAAATAGGTTTAACTATTGA", "aa": "MKRTLNIGIVLCENFLSDQQNAVDSYTQVYEDVRMFEFGLKLFQSLPFNIQETLIFCNAEQHKIVDKAAKKYKNTTVFFSRDTDVANVYEAKVFIQEKYKLTQDYKKRGVSSYYDSCCFILIEANRPLTAIKTVKSVYEKALIEKAAIAVLPYNGTLMNGNNDVVFSHLQDKNRFNY", "aa_hexdigest": "f6ec4c09c526c983565036166bbab5d9", "start_type": "ATG", "rbs_motif": null, "genes": [ "ispD" ], "truncated": "3-prime", "pseudogene": { "start": 142530, "stop": 143285, "observations": { "mutation": [ 143061 ], "stop": [ 143061 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47362", "query_cov": 1.0, "subject_cov": 0.7051792828685259, "identity": 1.0, "score": 354.0, "evalue": 1.16e-122, "gene_start": 1, "gene_end": 177, "reference_start": 1, "reference_end": 177, "reference_sequence": "MKRTLNIGIVLCENFLSDQQNAVDSYTQVYEDVRMFEFGLKLFQSLPFNIQETLIFCNAEQHKIVDKAAKKYKNTTVFFSRDTDVANVYEAKVFIQEKYKLTQDYKKRGVSSYYDSCCFILIEANRPLTAIKTVKSVYEKALIEKAAIAVLPYNGTLMNGNNDVVFSHLQDKNRFNYWKQSKAYEVQYPQAYTLNKLNQFSKQQFLRARSMLDLMKISNKSPLSIVDGSAYAFRVVTNLDFEILLGILKNG" }, "paralog": false, "identity": 0.9960159362549801, "score": 494.0, "evalue": 1.98e-180, "description": "Internal stop codon at 143061. Nonsense mutation around 143061.", "psc": { "uniref90_id": "UniRef90_P47362", "gene": "IspD", "product": "Uncharacterized protein MG116", "uniref50_id": "UniRef50_P75519", "cog_id": "COG1211", "cog_category": "I", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47362", "UniRef:UniRef50_P75519", "COG:COG1211", "COG:I" ] }, "pscc": { "uniref50_id": "UniRef50_P75519", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75519" ], "product": "Uncharacterized protein MG116 homolog" } }, "id": "KMADJIBOEG_306", "locus": "GCA000292445_00267" }, { "type": "cds", "sequence": "contig_1", "start": 143160, "stop": 143285, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGGACTTAATGAAGATTTCTAATAAATCACCCTTAAGTATTGTTGATGGTAGTGCTTATGCTTTTAGGGTTGTTACCAACCTTGATTTTGAAATTTTATTAGGTATTTTAAAAAATGGATAA", "aa": "MLDLMKISNKSPLSIVDGSAYAFRVVTNLDFEILLGILKNG", "aa_hexdigest": "8af0f9090e7692277155f57c0d623cce", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4497.279299999998, "isoelectric_point": 5.91951961517334 }, "id": "KMADJIBOEG_307", "locus": "GCA000292445_00268" }, { "type": "cds", "sequence": "contig_1", "start": 143278, "stop": 143481, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAAAATATTTTTCAATTAGTACAAAGTTTTGCTAAAACGCAAAATGTGCGAGCTAATTACACCTCACAAGACAAAAATATTAGTTTAGATTTTGTTAGTTTTGAAACAGTTAGTCAATCACTAACAGGTTTTCTAATTTTTAATAATTTCAATAAATTGTTACAACTAATTGAACTGATAAAACAAAAGCAAACATGA", "aa": "MDKNIFQLVQSFAKTQNVRANYTSQDKNISLDFVSFETVSQSLTGFLIFNNFNKLLQLIELIKQKQT", "aa_hexdigest": "695f58ce4a6841392ec802bbf66dbe4e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7789.825799999995, "isoelectric_point": 9.22402362823486 }, "id": "KMADJIBOEG_308", "locus": "GCA000292445_00269" }, { "type": "cds", "sequence": "contig_1", "start": 143569, "stop": 143922, "strand": "+", "frame": 1, "gene": null, "product": "MPN256 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47363", "UniRef:UniRef90_P47363" ], "nt": "TTGCCTGTTTCAGTTGCGGCTTTTAGCAACCAACAACTCAACCAAGTTTATCGCAATTCATCCACAAGTCCTTTGTATCTTAGTTATGTCAAACCAATCGAAGTTCAACAATTTTTTACACTTTCTCCATCCATTAACAATAATCCTTTTTTAAATCAAAATCCTTTAACTGAATCACCATTTGATAACAATAATCAGCTGTTTCAAGCAACAAAGAGCGTTGAACCTTCAATGGAAACAATGGAATTTTCCCGGTTTATGGATGAATTTGATCAGATTACAAAGAACTTTTCTGATATAGAGCTTGAACCTATGCAATTCACCCAAAGTTTTGATGATTGGAGCAAAGACTAG", "aa": "MPVSVAAFSNQQLNQVYRNSSTSPLYLSYVKPIEVQQFFTLSPSINNNPFLNQNPLTESPFDNNNQLFQATKSVEPSMETMEFSRFMDEFDQITKNFSDIELEPMQFTQSFDDWSKD", "aa_hexdigest": "20fe9c081e0b955f3813d61bd343d2d2", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 143278, "stop": 143922, "observations": { "mutation": [ 143479, 143500 ], "stop": [ 143479, 143500 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47363", "query_cov": 1.0, "subject_cov": 0.5467289719626168, "identity": 0.991, "score": 229.0, "evalue": 8.6e-75, "gene_start": 1, "gene_end": 117, "reference_start": 98, "reference_end": 214, "reference_sequence": "MDKNIFQLVQSFAKTQNVRANYTSQDKNISLDFVSFETVSQSLTGFLIFNNFNKLLQLIELIKQKQTWLYVDQLWIVDLSNNKALTDATVWIIKQEKLPVSVAAFSNQQLNQVYRNSSTSPLYLSYVKPIEVQQFFTLSPSINNNPFLNQNPLTESPFDNNNQLFQATKSVEPSMETMEFSRFMDEFDQITKNFSDIELEPMQFTQSFDDWSKD" }, "paralog": false, "identity": 0.9906542056074766, "score": 417.0, "evalue": 2.94e-151, "description": "Internal stop codon at 143479, 143500. Nonsense mutation around 143479, 143500.", "psc": { "uniref90_id": "UniRef90_P47363", "product": "MPN256 family protein", "uniref50_id": "UniRef50_P47363", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47363", "UniRef:UniRef50_P47363" ] }, "pscc": { "uniref50_id": "UniRef50_P47363", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47363" ], "product": "Uncharacterized protein MG117" } }, "id": "KMADJIBOEG_309", "locus": "GCA000292445_00270" }, { "type": "cds", "sequence": "contig_1", "start": 143903, "stop": 144643, "strand": "+", "frame": 2, "gene": null, "product": "NAD-dependent epimerase/dehydratase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557C9D", "UniRef:UniRef90_UPI0000557C9D" ], "nt": "ATGATTGGAGCAAAGACTAGGGTTGCAATAGTTGGCGGGATTGGTTACATAGGTAGTTGTTTTGCTAGTTTTATCAAAGAACAAAATGATAAGCTAATTGTTACTGTTATTGATAACAACAAAAATAACCATGTAATTAAACTCTTAAAAAAGATTGGAATTGAATTCTATTTTGCTGATTTACTAGATAGACATAAGCTAACTGAAGTAATTGCAGCAATTCAACCTGATGTGGTATTTCACTTTGCTGCTAAAACAAGTGTAAGTGAATCAGTACATAATCCATTGAAGTACTTTGATTGCAATGTAATTGGTACTTTAAACCTAATTAGTGCAATTAGTAACTTACAGAAGCCAATTAAATTATTTTTCGCTTCTAGTGCTGCAGTGTATGGTCAAACAACTAATAGTTACATTAGTGAAGAGATTGTAATAACTGAAACACAAGCAACCAATCCTTATGGATTGAGTAAGTTTTTAGATGAATTAATCTTAAATGCAGTTGCCAAAAATAGTCAACTACAAGTTGTTTGCTTACGCTTTTTTAATGTGGCAGGTGCAATTCTGCCATTTGGTAATTTTAATGGTAATACCACGCTTTTAATTCCTAACTTAGTAAAAGCCTTTTTAAAACAAACTCCCTTTTTTTTATATGGCAATGATTATGCAACTAAGGATGGTAGTTGCATAAGAGATTACATCCATGTTTATGATATATGTAATGCTCATTTCTTATTATGA", "aa": "MIGAKTRVAIVGGIGYIGSCFASFIKEQNDKLIVTVIDNNKNNHVIKLLKKIGIEFYFADLLDRHKLTEVIAAIQPDVVFHFAAKTSVSESVHNPLKYFDCNVIGTLNLISAISNLQKPIKLFFASSAAVYGQTTNSYISEEIVITETQATNPYGLSKFLDELILNAVAKNSQLQVVCLRFFNVAGAILPFGNFNGNTTLLIPNLVKAFLKQTPFFLYGNDYATKDGSCIRDYIHVYDICNAHFLL", "aa_hexdigest": "75b92aaa9fb3f53da333954460ea821d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0000557C9D", "query_cov": 0.8780487804878049, "subject_cov": 0.9432314410480349, "identity": 1.0, "score": 424.0, "evalue": 1.59e-149, "valid": true, "product": "NAD-dependent epimerase/dehydratase family protein", "uniref50_id": "UniRef50_UPI0000557C9D" }, "pscc": { "uniref50_id": "UniRef50_UPI0000557C9D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557C9D" ], "product": "NAD-dependent epimerase/dehydratase family protein" }, "genes": [], "id": "KMADJIBOEG_310", "locus": "GCA000292445_00271" }, { "type": "cds", "sequence": "contig_1", "start": 144689, "stop": 144925, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000319466E", "UniRef:UniRef90_UPI000319466E" ], "nt": "TTGGGGAGTGGGATAGGAACTTCTAATTTAGAAGTTATTGATATTGCTAAAAAAGTGTTTTATCCTAGTAGATTAAATTTAGAAATTAGACCAAAAAGAAGCGGAGATCCAGCAATTTTAGTAGCAAATGTTGCTAAAGCAAAACAAACCTTTCAATTCAAAATAACGCGTAATTTGAAAGATATGATAAGTGATGAGCGTAATTTTTATGAGAATTTTTATAATGACGCTTATTAA", "aa": "MGSGIGTSNLEVIDIAKKVFYPSRLNLEIRPKRSGDPAILVANVAKAKQTFQFKITRNLKDMISDERNFYENFYNDAY", "aa_hexdigest": "ec23c94f196962c16cbae406d5f740f6", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000319466E", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.987, "score": 153.0, "evalue": 3.31e-47, "valid": true, "uniref50_id": "UniRef50_UPI000319466E" }, "pscc": { "uniref50_id": "UniRef50_UPI000319466E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000319466E" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8933.104899999998, "isoelectric_point": 9.345095252990724 }, "id": "KMADJIBOEG_311", "locus": "GCA000292445_00272" }, { "type": "cds", "sequence": "contig_1", "start": 144961, "stop": 146103, "strand": "+", "frame": 1, "gene": "nupO", "product": "Putative carbohydrate transport ATP-binding protein MG119", "db_xrefs": [ "COG:COG3845", "COG:F", "GO:0005524", "GO:0005886", "GO:0016887", "KEGG:K23537", "SO:0001217", "UniRef:UniRef50_P47365", "UniRef:UniRef90_P47365" ], "nt": "ATGGAGCATATCTCCAAAAGTTTTGACAATGGCAAAATTAAGGCTAATGTTGATGTTAGCTTAGTTGTTTATGAAAATACTGTCCACACCATTTTGGGGGAGAATGGTGCAGGAAAATCAACCCTGACTTCGATTTTATTTGGTTTATATAAACCTGATAGTGGCAAGATCTTTATTGGTGAAAAGCAAGTAAATTTTAAATCTTCTAAAGATGCAGTAAAACATAAAATCGGAATGGTGCACCAGCACTTTAAGTTAATAGAAAACTACACGGTTTTAGATAACATCATTCTAGGGAATGAAAGTAGGTTTGGGTTTTTACCTTTAATTAATCGTAAAGTAAGTGAAGCAAAGATTAAAACCATCATGGAAAAATATGGAATCTTTGTTGATCTTAAACAAAAAGTTAGTAACTTAACAGTAAGTCAGCAACAACGGGTTGAGATCCTAAAGGTTTTATTTCGTGATAGTAATATCCTCATCTTTGATGAACCCACTGCAGTTTTAAGTGATCTTGAAATTCAAAACTTTCTCAAGATTATTGCTAACTTTAAAAAGCTAGGAAAAACAATTGTTTTAATCTCTCATAAATTAAATGAAATTAAACAAGTTGCTGATACAGCTACTGTCTTAAGACTTGGCAAGGTAGTTGGTAGATTTGATGTTAAAACAACACCAGTTGATAAGATTGCGCTTTTAATGATGGGCAAAGAGTTAAAACAAACTAAAAACACCACAGATTTTGTTGCTAAAGATGAACCTGTTTTAAAAGTTCAAAACCTGAATTTGTTTCTCAATAAATCTTTAGCATACAAGTTCTTAGTGAGATGCAATAACATCCATAAAGCCCAACAAATTAAGAAAAATAAACCATTAAAAGACTTATGGATAATTAGTTTTTTAAATAAACTAACCACCAGTAACAAAACCCCTAAATTAGTAAAAGGCTTGATTAATAAGTTAGGACTTTCCTATCAAGAAAATACAGATGAAACCATTAGTTTTGCTATCCATAAGGGAGAAATTTTTGCTATTGCTGGGGTTGAGGGTAATGGTCAAAGTCAGCTTGTTAATTTAATTTGTGGAATTGAAAAAGCTGCTAGTAATAAGTTAATTTTTAACAATATTGATATCTCAAGATGA", "aa": "MEHISKSFDNGKIKANVDVSLVVYENTVHTILGENGAGKSTLTSILFGLYKPDSGKIFIGEKQVNFKSSKDAVKHKIGMVHQHFKLIENYTVLDNIILGNESRFGFLPLINRKVSEAKIKTIMEKYGIFVDLKQKVSNLTVSQQQRVEILKVLFRDSNILIFDEPTAVLSDLEIQNFLKIIANFKKLGKTIVLISHKLNEIKQVADTATVLRLGKVVGRFDVKTTPVDKIALLMMGKELKQTKNTTDFVAKDEPVLKVQNLNLFLNKSLAYKFLVRCNNIHKAQQIKKNKPLKDLWIISFLNKLTTSNKTPKLVKGLINKLGLSYQENTDETISFAIHKGEIFAIAGVEGNGQSQLVNLICGIEKAASNKLIFNNIDISR", "aa_hexdigest": "4598ec2f7ae0201ce96797f67eb6b4a9", "start_type": "ATG", "rbs_motif": null, "genes": [ "nupO" ], "truncated": "3-prime", "pseudogene": { "start": 144940, "stop": 146406, "observations": { "mutation": [ 146101, 146236 ], "stop": [ 146101, 146236 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47365", "query_cov": 1.0, "subject_cov": 0.6737588652482269, "identity": 0.995, "score": 727.0, "evalue": 1.9e-261, "gene_start": 1, "gene_end": 380, "reference_start": 8, "reference_end": 387, "reference_sequence": "MEKVAFKMEHISKSFDNGKIKANVDVSLVVYENTVHTILGENGAGKSTLTSILFGLYKPDSGKIFIGEKQVNFKSSKDAVKHKIGMVHQHFKLIENYTVLDNIILGNESRFGFLPLINRKVSEAKIKTIMEKYGIFVDLKQKVSNLTVGQQQRVEILKVLFRDSNILIFDEPTAVLSDLEIQNFLKIIANFKKLGKTIVLISHKLNEIKQVADTATVLRLGKVVGSFDVKTTPVDKIALLMMGKELKQTKNTTDFVAKDEPVLKVQNLNLFLNKSLAYKFLVRCNNIHKAQQIKKNKPLKDLWIISFLNKLTTSNKTPKLVKGLINKLGLSYQENTDETISFAIHKGEIFAIAGVEGNGQSQLVNLICGIEKAASNKLIFNNIDISRWSIRKRINAGISFVLEDRHKYGLILDQTVRFNTVNNQINNRPFSSWNFLKPMEIALYSNTIIKKFDVRGSAEGSAVVRRLSGGNQQKLIIGREMTKQNDLLVLAQVTRGLDIGAIAFIHENILLAKANNKAILLVSYELDEILALADTVAVINKGRIVGMGKRDLMDRQSIGRLIMQ" }, "paralog": false, "identity": 0.9897540983606558, "score": 932.0, "evalue": 0.0, "description": "Internal stop codon at 146101, 146236. Nonsense mutation around 146101, 146236.", "psc": { "uniref90_id": "UniRef90_P47365", "gene": "NupO", "product": "Putative carbohydrate transport ATP-binding protein MG119", "uniref50_id": "UniRef50_P47365", "cog_id": "COG3845", "cog_category": "F", "kegg_orthology_id": "K23537", "go_ids": [ "GO:0005886", "GO:0005524", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47365", "UniRef:UniRef50_P47365", "COG:COG3845", "COG:F", "KEGG:K23537", "GO:0005886", "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47365", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47365" ], "product": "Putative carbohydrate transport ATP-binding protein MG119" } }, "id": "KMADJIBOEG_312", "locus": "GCA000292445_00273" }, { "type": "cds", "sequence": "contig_1", "start": 146254, "stop": 146634, "strand": "+", "frame": 1, "gene": "mglA", "product": "Galactose/methyl galactoside import ATP-binding protein MglA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M965" ], "nt": "ATGGAGATTGCTCTTTATAGCAACACTATTATTAAAAAGTTTGATGTTAGGGGCAGTGCTGAGGGTAGTGCTGTTGTAAGAAGACTTTCAGGTGGTAATCAACAGAAACTAATTATTGGTCGAGAAATGACCAAACAAAATGACCTTTTGGTGTTAGCACAAGTAACCAGAGGCCTTGATATTGGTGCTATTGCTTTTATCCATGAAAACATCTTATTAGCTAAAGCTAATAATAAAGCTATCTTATTGGTTTCATATGAACTTGATGAGATCTTAGCACTTGCTGATACAGTGGCTGTTATCAATAAGGGGAGAATAGTTGGTATGGGAAAAAGAGATTTAATGGATCGCCAATCGATAGGTAGATTAATAATGCAATAA", "aa": "MEIALYSNTIIKKFDVRGSAEGSAVVRRLSGGNQQKLIIGREMTKQNDLLVLAQVTRGLDIGAIAFIHENILLAKANNKAILLVSYELDEILALADTVAVINKGRIVGMGKRDLMDRQSIGRLIMQ", "aa_hexdigest": "e869337ed1f0987f1d2aa27fd769dede", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A223M965", "query_cov": 0.9126984126984127, "subject_cov": 0.9274193548387096, "identity": 0.574, "score": 123.0, "evalue": 4.79e-34, "valid": false, "product": "Galactose/methyl galactoside import ATP-binding protein MglA", "uniref50_id": "UniRef50_A0A223M965" }, "pscc": { "uniref50_id": "UniRef50_A0A223M965", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M965" ], "product": "Galactose/methyl galactoside import ATP-binding protein MglA" }, "genes": [ "mglA" ], "id": "KMADJIBOEG_313", "locus": "GCA000292445_00274" }, { "type": "cds", "sequence": "contig_1", "start": 146725, "stop": 147165, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAGAAGAAGTATCCTCTCTTCAGTGGTTTTGATAATCCTCTCTTTTCTTATATCGTTTTTACTGATTATTTCAATTCCTGGAGGTAGAGGTGCGAGCTTCTTTGCACTGTTTACTAAGTTATTTTTAGATAACACTAATACTGAAAATTTCTTAAGACAGATTGCTATTTATATCCTAGCTGGATTAGCATTTAGTTTCTGTATGAGTGTTGGTATTTTCAACATTGGTATCTCAGGGCAGATGATGGCTGGAGCCATCTTTGGGTTTTTAATGATTCTCAAGGTGTTTCCAAGTTCATTTCGACCTGGTTTTGGAGGTCAGATTATTACTGTATTATTGATGGTAATAGGTAGTGTTAGTGTGGCAGTTGTTGTTGCAACTTTAAAGATTTTCTTCAAGGTTAATGAAGTTGTAAGTGCAATTATGTTGAACTGA", "aa": "MQRRSILSSVVLIILSFLISFLLIISIPGGRGASFFALFTKLFLDNTNTENFLRQIAIYILAGLAFSFCMSVGIFNIGISGQMMAGAIFGFLMILKVFPSSFRPGFGGQIITVLLMVIGSVSVAVVVATLKIFFKVNEVVSAIMLN", "aa_hexdigest": "5029e9cca108206d566a5d1a98f2de81", "start_type": "ATG", "rbs_motif": "ATATA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15812.016799999998, "isoelectric_point": 10.531829261779784 }, "id": "KMADJIBOEG_314", "locus": "GCA000292445_00275" }, { "type": "cds", "sequence": "contig_1", "start": 147172, "stop": 147306, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTATTAGTGCTTATTTAGTAGAGACTTACATTAAAAATAATAGTGGGGGTACAGCCCAATTCTTTTCCTTACCACTCCCTGATGAATTTGCTTTATATAACTTCTCTCCTTTAACAAAAAAGTTTGGTTGA", "aa": "MLISAYLVETYIKNNSGGTAQFFSLPLPDEFALYNFSPLTKKFG", "aa_hexdigest": "ef71a9c424543b530de92ee293cb34f4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4934.6151, "isoelectric_point": 5.936628150939941 }, "id": "KMADJIBOEG_315", "locus": "GCA000292445_00276" }, { "type": "cds", "sequence": "contig_1", "start": 147346, "stop": 147774, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCAGTAGTATTAAAATACACAGTTTTTGGACACAAATTAAAGTCAATTGGTAGTAGTGTATTTGGTTCTCAGGCAATGGGTTTTAATGTTAAAAAATACCAGTTCTTATCGTTTATTATCTCAGGAATTTTATCAGGACTATTAGCAACGGTTGTTTACACTGCATCAACTGAAAAAGTATTGACATTTAACAATGTTGGGGATAGTGCTATTTCAGCAGTACCAGCTACTGGTTTTGATGGGATTGCGATTGGTTTAATTGCTTTAAATAACCCCTTTAGGATTGTTATTGTTTCTGTTCTTATTGCTTTTGTTAACATTGGGGCAAGACCTGCTAATTTAAACCCTAGTACTGCTAGTTTAGTTTTAGGAATCATGATGTATTTTGCTGCACTTTATAACCTAATGGTTTACTTTAAACCATGA", "aa": "MAVVLKYTVFGHKLKSIGSSVFGSQAMGFNVKKYQFLSFIISGILSGLLATVVYTASTEKVLTFNNVGDSAISAVPATGFDGIAIGLIALNNPFRIVIVSVLIAFVNIGARPANLNPSTASLVLGIMMYFAALYNLMVYFKP", "aa_hexdigest": "b757aba318562ca10a18bd74a9e7ffa6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15091.734799999977, "isoelectric_point": 9.7943754196167 }, "id": "KMADJIBOEG_316", "locus": "GCA000292445_00277" }, { "type": "cds", "sequence": "contig_1", "start": 148021, "stop": 148203, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGCAATCAAAAGTGGCAATATTAAACCTTCAACTACTTTTAAGTTGGAATTTGTTAATTTTAAACACCAACAGAAGTTTGTATTAAATTGGTTTAAAAATGAAAGTGAATCACTGCGTGATTTCCAATCACAGTTTGAGAGAATCAATAAGTTAGTGGAAAGGGAGTTTGTTAAGTAA", "aa": "MNAIKSGNIKPSTTFKLEFVNFKHQQKFVLNWFKNESESLRDFQSQFERINKLVEREFVK", "aa_hexdigest": "97948b4f1e5b7e13d4e01cde7bc1ae90", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7282.256099999995, "isoelectric_point": 9.77445468902588 }, "id": "KMADJIBOEG_317", "locus": "GCA000292445_00278" }, { "type": "cds", "sequence": "contig_1", "start": 148206, "stop": 148403, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAGTTTAGCACAATTAGAAAGTTGGTTTTTTATCGCTCCAGCACTGCTTTTAGCAGTATTGAGTGGTTATCTTGCTGAACGCGTTGGGATCATTAATATTGCTATTAATGGTGGAATGGTGTTTGGTGGGTTATTTATGGCACTCTTATCGTATGGATTTACAAATAACTTAAACCAATCCGCTCCCTCCTGA", "aa": "MLSLAQLESWFFIAPALLLAVLSGYLAERVGIINIAINGGMVFGGLFMALLSYGFTNNLNQSAPS", "aa_hexdigest": "fd18b1108f04c3f653ff47333939cb4d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6908.0662999999995, "isoelectric_point": 4.529692649841309 }, "id": "KMADJIBOEG_318", "locus": "GCA000292445_00279" }, { "type": "cds", "sequence": "contig_1", "start": 148503, "stop": 148925, "strand": "+", "frame": 3, "gene": "nupQ", "product": "putative protein MG121", "db_xrefs": [ "COG:COG1079", "COG:F", "GO:0005886", "GO:0022857", "KEGG:K23536", "SO:0001217", "UniRef:UniRef50_P47367", "UniRef:UniRef90_P47367" ], "nt": "GTGGGAACTGGGATTAACTTGTTGGCTAGCGGAATTACCCTTTTTATTAGTCAGAATGCTGCTAGTTTGTTTTCCGATACTACCTTAAGAGTAAGGTACTTATTTCCCATCCAAACTACTGTTAGTATAGAAGCAATTGGTGTGTTTGTTTTTAGTTTACTTCTGATTGGGTTTGTATGGTACTTGATGAGTTTTACTAAAACTGGGTTGAGATACCGTGCGGTAGGTGAGAATCCTAATGTAATTGATACCCAAGGGATTAGTGTTTACAAATACCAATGGATAGGCGCAATTTGTTCAATGATGGTAGCTGGATTGAGTGGTAGTTTGTTTGTTTTAAGTGTTTCTAACTTTCCCTTTAACAGCGGAGATGTAAATGGCTTGGGTTTTATTGCTATTGCCATTATGATTATCTCAATGTGA", "aa": "MGTGINLLASGITLFISQNAASLFSDTTLRVRYLFPIQTTVSIEAIGVFVFSLLLIGFVWYLMSFTKTGLRYRAVGENPNVIDTQGISVYKYQWIGAICSMMVAGLSGSLFVLSVSNFPFNSGDVNGLGFIAIAIMIISM", "aa_hexdigest": "eca65363cbce6200ca83e86a8fd98295", "start_type": "GTG", "rbs_motif": null, "genes": [ "nupQ" ], "truncated": "both", "pseudogene": { "start": 148206, "stop": 149126, "observations": { "mutation": [ 148401, 148923 ], "stop": [ 148401, 148923 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47367", "query_cov": 1.0, "subject_cov": 0.45751633986928103, "identity": 0.993, "score": 266.0, "evalue": 7.84e-88, "gene_start": 1, "gene_end": 140, "reference_start": 100, "reference_end": 239, "reference_sequence": "MLSLAQLESWFFIAPALLLAVLSGYLAERVGIINIAINGGMVFGGLFMALLSYGFTNNLNQSAPSWSLFITIPLSVLFSSVIGCLFALAAVKLRADHVIVGTGINLLASGITLFISQNAASLFSDTTLRVRYLFPIQTTVSIEAIGVFVFSLLLIGFVWYLMSFTKTGLRYRAVGENPNVIDTQGISVYKYQWIGAICSMMVAGLSGSLFVLSVSNFPFNSGDVNGLGFIAIAIMIISMWRIIPSIFIGLIFAYAYVFTNSQIGSNSNSYLLRTIPFIISLLVMLLFGFLNVAPKNIGKHFDKGLR" }, "paralog": false, "identity": 0.9901960784313726, "score": 581.0, "evalue": 1.9e-214, "description": "Internal stop codon at 148401, 148923. Nonsense mutation around 148401, 148923.", "psc": { "uniref90_id": "UniRef90_P47367", "gene": "NupQ", "product": "Uncharacterized protein MG121", "uniref50_id": "UniRef50_P47367", "cog_id": "COG1079", "cog_category": "F", "kegg_orthology_id": "K23536", "go_ids": [ "GO:0005886", "GO:0022857" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47367", "UniRef:UniRef50_P47367", "COG:COG1079", "COG:F", "KEGG:K23536", "GO:0005886", "GO:0022857" ] }, "pscc": { "uniref50_id": "UniRef50_P47367", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47367" ], "product": "Uncharacterized protein MG121" } }, "id": "KMADJIBOEG_319", "locus": "GCA000292445_00280" }, { "type": "cds", "sequence": "contig_1", "start": 149166, "stop": 149327, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAAAAACCTGGTGGTGATTGAATCACCCAATAAAGTTAAAACATTAAAACAATATCTTCCTAGTGATGAATTTGAGATAGTCTCAACCGTTGGTCACATCAGAGAAATGGTGTATAAAAACTTTGGTTTTGATGAAAATACCTATACCCCTATCTGA", "aa": "MIKNLVVIESPNKVKTLKQYLPSDEFEIVSTVGHIREMVYKNFGFDENTYTPI", "aa_hexdigest": "70b39fdba60de8fd39e6d8f91219a05a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6177.061699999999, "isoelectric_point": 5.644021034240722 }, "id": "KMADJIBOEG_320", "locus": "GCA000292445_00281" }, { "type": "cds", "sequence": "contig_1", "start": 149496, "stop": 150935, "strand": "+", "frame": 3, "gene": "topA", "product": "type I DNA topoisomerase", "db_xrefs": [ "COG:COG0551", "COG:L", "EC:5.6.2.1", "GO:0003677", "GO:0003917", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0006265", "GO:0046872", "KEGG:K03168", "SO:0001217", "UniRef:UniRef50_P47368", "UniRef:UniRef90_P47368" ], "nt": "TTGGATCAAAAAGATAAAGCTAAGTGCAAACGAATCACTTTCAATGAGATCACTAAAAAAGCAGTAGTAGATGCATTAAAACAACCGCGTAACATCGATCTTAACTGGGTTGAAAGTCAGTTTGCCCGCCAAATCCTTGACAGGATGATTGGTTTTAGATTATCAAGATTATTAAATAGTTATCTGCAAGCAAAGTCTGCAGGTAGAGTTCAATCAGTGGCTTTGCGCTTTCTTGAGGAAAGAGAAAAGGAGATAGCTAAGTTTGTTCCGCGTTTTTGGTGGACAGTTGATGTTTTATTAAACAAAGAAAATAACCAAAAAGTAGTTTGTGCAAACAAGTCTATTCCTTTGGTTTTAAGAGAAATTAACCCTGAATTAAGTGCTAGTTTAAAACTGGATTTTGAAGCTGCTGAAAACGTATCAGGAATTGACTTTTTAAATGAAGCTTCAGCAACCAGATTTGCCAACCAACTGACTGGCGAATATGAAGTTTATTTTATTGATGAACCTAAGATTTACTATTCATCTCCAAACCCAGTTTATACCACCGCTTCACTTCAAAAGGATGCAATTAATAAGTTAGGATGGTCTTCCAAAAAAGTAACAATGGTGGCCCAAAGACTGTATGAAGGGATTAGTGTTAATGGGAAACAAACTGCATTAATTAGTTATCCAAGAACTGATTCAATTAGGATTTCAAACCAATTTCAATCAGAGTGTGAAAAGTACATTGAAAAGGAGTTTGGAAGTCATTATTTAGCTGATAAAAATAAGTTAAAAAGACATAAAAAGGATGAGAAAATCATCCAAGATGCCCATGAAGGGATCCATCCTACTTACATTACTATTACCCCCAATGATCTGAAAAACGGGGTGAAACGCGATGAGTTTCTCCTTTATCGTTTAATATGGATTAGAACAGTTGCTAGTTTAATGGCAGATGCTAAAACATCAAGAACTATTGTTCGTTTTATAAACCAAAAAAACAAGTTTTATACCTCTTCAAAATCACTTTTATTTGATGGTTATCAAAGGTTATATGAAGAGATTAAACCTAATACTAAAGATGAACTTTACATTGATCTTAGTAAGCTTAAAATTGGTGATAAATTTAGTTTTGAAAAGATCAGCGTTAATGAGCATAAAACCAACCCACCACCACGTTACACCCAAGCTAGTTTAATTGAAGAGCTTGAAAAATCTAACATCGGTCGTCCCTCTACTTATAACACTATGGCCAGTGTTAATCTAGAAAGGGGCTATGCTAACTTAGTGAACCGATTTTTTTATATCACTGAGCTTGGTGAAAAAGTTAATAATGAACTTTCCAAGCATTTTGGGAATGTAATTAATAAAGAATTTACCAAGAAGATGGAAAAATCTTTGGATGAAATTGCTGAAAACAAAGTAAACTATCAAGAATTTCTTAAGCAGTTTTGA", "aa": "MDQKDKAKCKRITFNEITKKAVVDALKQPRNIDLNWVESQFARQILDRMIGFRLSRLLNSYLQAKSAGRVQSVALRFLEEREKEIAKFVPRFWWTVDVLLNKENNQKVVCANKSIPLVLREINPELSASLKLDFEAAENVSGIDFLNEASATRFANQLTGEYEVYFIDEPKIYYSSPNPVYTTASLQKDAINKLGWSSKKVTMVAQRLYEGISVNGKQTALISYPRTDSIRISNQFQSECEKYIEKEFGSHYLADKNKLKRHKKDEKIIQDAHEGIHPTYITITPNDLKNGVKRDEFLLYRLIWIRTVASLMADAKTSRTIVRFINQKNKFYTSSKSLLFDGYQRLYEEIKPNTKDELYIDLSKLKIGDKFSFEKISVNEHKTNPPPRYTQASLIEELEKSNIGRPSTYNTMASVNLERGYANLVNRFFYITELGEKVNNELSKHFGNVINKEFTKKMEKSLDEIAENKVNYQEFLKQF", "aa_hexdigest": "c0057832d8c57db8df47d4aca2234c5a", "start_type": "TTG", "rbs_motif": null, "genes": [ "topA" ], "truncated": "both", "pseudogene": { "start": 149196, "stop": 151225, "observations": { "deletion": [ 150996 ], "mutation": [ 149325, 149334, 150933 ], "stop": [ 149325, 149334, 150933 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47368", "query_cov": 1.0, "subject_cov": 0.6755994358251057, "identity": 0.998, "score": 937.0, "evalue": 0.0, "gene_start": 1, "gene_end": 479, "reference_start": 111, "reference_end": 589, "reference_sequence": "MIKNLVVIESPNKVKTLKQYLPSDEFEIVSTVGHIREMVYKNFGFDENTYTPIWEDWTKNKQKNPKQKHLLSKFEIIKSIKAKASDAQNIFLASDPDREGEAISWHVYDLLDQKDKAKCKRITFNEITKKAVVDALKQPRNIDLNWVESQFARQILDRMIGFRLSRLLNSYLQAKSAGRVQSVALRFLEEREKEIAKFVPRFWWTVDVLLNKENNQKVVCANKSIPLVLREINPELSASLKLDFEAAENVSGIDFLNEASATRFANQLTGEYEVYFIDEPKIYYSSPNPVYTTASLQKDAINKLGWSSKKVTMVAQRLYEGISVNGKQTALISYPRTDSIRISNQFQSECEKYIEKEFGSHYLADKNKLKRHKKDEKIIQDAHEGIHPTYITITPNDLKNGVKRDEFLLYRLIWIRTVASLMADAKTSRTIVRFINQKNKFYTSSKSLLFDGYQRLYEEIKPNTKDELYIDLSKLKIGDKFSFEKISVNEHKTNPPPRYTQASLIEELEKSNIGRPSTYNTMASVNLERGYANLVNRFFYITELGEKVNNELSKHFGNVINKEFTKKMEKSLDEIAENKVNYQEFLKQFWTNFKSDVKLAENSIQKVKKEKELVERDCPKCNQPLVYRYTKRGNEKFVGCSDFPKCKYSEFSNPKPKLTLETLDELCPECNNKLVKRRTKFNAKKTFIGCSNFPNCRFIKKDNAAEFKQ" }, "paralog": false, "identity": 0.9807692307692307, "score": 1274.0, "evalue": 0.0, "description": "Internal stop codon at 149325, 149334, 150933. Frameshift due to deletion around 150996. Nonsense mutation around 149325, 149334, 150933.", "psc": { "uniref90_id": "UniRef90_P47368", "gene": "topA", "product": "type I DNA topoisomerase", "ec_ids": [ "5.6.2.1" ], "uniref50_id": "UniRef50_P47368", "cog_id": "COG0551", "cog_category": "L", "kegg_orthology_id": "K03168", "go_ids": [ "GO:0003677", "GO:0003917", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0006265", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47368", "EC:5.6.2.1", "UniRef:UniRef50_P47368", "COG:COG0551", "COG:L", "KEGG:K03168", "GO:0003677", "GO:0003917", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0006265", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47368", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47368" ], "product": "DNA topoisomerase 1" } }, "id": "KMADJIBOEG_321", "locus": "GCA000292445_00282" }, { "type": "cds", "sequence": "contig_1", "start": 151001, "stop": 151294, "strand": "+", "frame": 2, "gene": null, "product": "topoisomerase DNA-binding C4 zinc finger domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED90A12", "UniRef:UniRef90_UPI001ED90A12" ], "nt": "TTGGTTGAAAGAGATTGTCCTAAATGTAATCAACCGTTGGTATATCGTTACACCAAAAGAGGTAATGAGAAGTTTGTTGGTTGTAGTGATTTTCCTAAGTGTAAATACAGTGAGTTTAGTAATCCTAAACCAAAACTAACCTTGGAAACACTTGATGAATTGTGTCCTGAGTGTAACAATAAACTGGTTAAGAGGAGAACTAAATTTAACGCTAAAAAGACCTTTATAGGTTGCAGTAATTTCCCTAACTGCCGTTTTATCAAAAAGGATAATGCTGCTGAATTTAAACAATAA", "aa": "MVERDCPKCNQPLVYRYTKRGNEKFVGCSDFPKCKYSEFSNPKPKLTLETLDELCPECNNKLVKRRTKFNAKKTFIGCSNFPNCRFIKKDNAAEFKQ", "aa_hexdigest": "0afa59ffeba519a03a5f69fa0caf6026", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001ED90A12", "query_cov": 0.8762886597938144, "subject_cov": 1.0, "identity": 0.988, "score": 150.0, "evalue": 1.04e-45, "valid": true, "product": "topoisomerase DNA-binding C4 zinc finger domain-containing protein", "uniref50_id": "UniRef50_UPI001ED90A12" }, "pscc": { "uniref50_id": "UniRef50_UPI001ED90A12", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED90A12" ], "product": "topoisomerase DNA-binding C4 zinc finger domain-containing protein" }, "genes": [], "id": "KMADJIBOEG_322", "locus": "GCA000292445_00283" }, { "type": "cds", "sequence": "contig_1", "start": 151272, "stop": 152264, "strand": "+", "frame": 3, "gene": null, "product": "MPN262 family protein", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_P47369", "UniRef:UniRef90_P47369" ], "nt": "ATGCTGCTGAATTTAAACAATAACAGCGTTCTTATTGTTGCGTTTGTAATTGTTTCTTTATTCTTTCTAATAATTGTTGGGTTTGCTTTAAATTTAGCAATTGCTTTTTCACTCCATTTAAAGCAGAATAAAAATAACAAAAAATACATCTTAAATGACCAACAGATCCAGTTAAGATTAACTGAAAAACAAGCCCAATTAACAACTTTACTTAACTTTTATCAACAAAAAATTGAAAGTGTAAACAGAGAAAAAAGTTGGTTAGAAAGTCAGTTACAGGTAATTGATAAAAAGGATTTAAAGCAAGCGCAAAAGTTAACTTTACATTTAAAAAAAGACCAAATATTAGCTCAACTTAATGAAAAGCTGATTCAAAAAAAAGTTGATCAGCCTTTAGTTAATGAACTACAGAAAATCAAACTTTCCTATCTTGAAAGGTTAGTTGATCAAAAGATTAAACTCAGTGAAAATAATTTCAAAAGTGCTTTTCTTAAAACGAAGGTAAAAGAGACAGCATTTAATATCTTTGCAGCTAAAAACAAGGTTAACTGGGAGTATTTTAAACAGGTGTGTGATGCTGATTGCACTTTAAAAAACTTAGAAGATGAAGTGGAAATTACTTTTTCTAATTGGAGTTATTTGAGAAGGATGCAAGCTCTATTAGCGTTTGAAAAACTAATTAGCAAAATCAAAACAGTCAAAATTAATGAATTGGTTATTAATGAAACTTTAGATGAAGTGAAAAACGAAATTAGCCAAACTGCTTTTCAAGCGGGTGAAAAAATAGTTAAGGAATTTCAGATAACTAATTTAAACGAGCAAATTACCAGACTAATTGGCTTGCAAAAATACTATTTTGGCACTGATCAGCTCAATCTCTTAGAACTCGCGGTATTAACAACCAAATTAGTAATTTTACTGAACAAAAAGTTCAAACTAGATTTAGATTTAGAACTTCTTAAAGCAGCTAGCTTGTTTAACTATTTAAAGTGA", "aa": "MLLNLNNNSVLIVAFVIVSLFFLIIVGFALNLAIAFSLHLKQNKNNKKYILNDQQIQLRLTEKQAQLTTLLNFYQQKIESVNREKSWLESQLQVIDKKDLKQAQKLTLHLKKDQILAQLNEKLIQKKVDQPLVNELQKIKLSYLERLVDQKIKLSENNFKSAFLKTKVKETAFNIFAAKNKVNWEYFKQVCDADCTLKNLEDEVEITFSNWSYLRRMQALLAFEKLISKIKTVKINELVINETLDEVKNEISQTAFQAGEKIVKEFQITNLNEQITRLIGLQKYYFGTDQLNLLELAVLTTKLVILLNKKFKLDLDLELLKAASLFNYLK", "aa_hexdigest": "c58c3753d191e66d4932328d0020581d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 151272, "stop": 152567, "observations": { "mutation": [ 152262 ], "stop": [ 152262 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47369", "query_cov": 1.0, "subject_cov": 0.7006369426751592, "identity": 0.997, "score": 595.0, "evalue": 9.86e-212, "gene_start": 1, "gene_end": 330, "reference_start": 1, "reference_end": 330, "reference_sequence": "MLLNLNNNSVLIVAFVIVSLFFLIIVGFALNLAIAFSLHLKQNKNNKKYILNDQQIQLRLTEKQAQLTTLLNFYQQKIESVNREKSWLESQLQVIDKKDLKQAQKLTLHLKKDQILAQLNEKLIQKKVDQPLVNELQKTKLSYLERLVDQKIKLSENNFKSAFLKTKVKETAFNIFAAKNKVNWEYFKQVCDADCTLKNLEDEVEITFSNWSYLRRMQALLAFEKLISKIKTVKINELVINETLDEVKNEISQTAFQAGEKIVKEFQITNLNEQITRLIGLQKYYFGTDQLNLLELAVLTTKLVILLNKKFKLDLDLELLKAASLFNYLKWVDNNQFFQILNTKLNQLLISDQVIAIIQQQELSFYPDQYGMLINGVKTMIREHNTIDFEKLVFLNSTKLIDNFYLYDLNMIHAVEYNNCFYYFVSVKPFEIKSLAELDLFVVLLKTFLAKKQKQNPKAVKLFITTKILAI" }, "paralog": false, "identity": 0.9930394431554525, "score": 827.0, "evalue": 1.54e-305, "description": "Internal stop codon at 152262. Nonsense mutation around 152262.", "psc": { "uniref90_id": "UniRef90_P47369", "product": "MPN262 family protein", "uniref50_id": "UniRef50_P47369", "go_ids": [ "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47369", "UniRef:UniRef50_P47369", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47369", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47369" ], "product": "Uncharacterized protein MG123" } }, "id": "KMADJIBOEG_323", "locus": "GCA000292445_00284" }, { "type": "cds", "sequence": "contig_1", "start": 152385, "stop": 152687, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATTAATGGAGTGAAAACAATGATTAGAGAGCATAACACCATTGATTTTGAAAAACTGGTTTTTCTCAACTCAACAAAACTTATTGATAATTTTTATCTCTATGATTTAAATATGATCCATGCAGTTGAATATAACAACTGCTTTTATTACTTTGTTAGTGTTAAACCCTTTGAGATTAAGTCATTAGCAGAACTTGATCTTTTTGTTGTTTTGTTAAAAACTTTTTTAGCTAAAAAGCAAAAGCAAAATCCAAAAGCGGTTAAACTGTTTATCACTACCAAAATTTTAGCGATCTAA", "aa": "MLINGVKTMIREHNTIDFEKLVFLNSTKLIDNFYLYDLNMIHAVEYNNCFYYFVSVKPFEIKSLAELDLFVVLLKTFLAKKQKQNPKAVKLFITTKILAI", "aa_hexdigest": "2a9f4881a699bb72b0bf3c1e1bd5ef9b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11768.906399999985, "isoelectric_point": 9.212806129455569 }, "id": "KMADJIBOEG_324", "locus": "GCA000292445_00285" }, { "type": "cds", "sequence": "contig_1", "start": 153048, "stop": 153737, "strand": "+", "frame": 3, "gene": "cof", "product": "Putative phosphatase MG125", "db_xrefs": [ "COG:COG0561", "COG:HR", "EC:3.1.3.-", "GO:0000287", "GO:0005829", "GO:0016791", "SO:0001217", "UniRef:UniRef50_P47371", "UniRef:UniRef90_P47371" ], "nt": "ATGATTGATCTGCTTGGTTTGGATCTGGATGGAACGTTATTATCTAAAACTAAAAAAATTAACAATCCATCAAAATTAGCATTAACTAATTTAATTGCTAAAAAACCAAGTTTAAAGGTGATGATTTTAACTGGTAGATCAGTTTTTTCTACTCTAAAACACGTTGAAAAGCTGAACAGTTTGTTTAAAAAACCAATTGTTGATTATTTTTGTTGTTATGGGGGTGCTAAACTTTATCAAATTGAAGCAAATAAGCCACAAGAAAGATACAAGTTTTGCTTGGAAAACAGTGTTGTTGAAACTACCTTTAGTATTATCAAAAAACACCGCGGATTATGTTTAGCTTACTTAGATAGTTATGTCTCTCCTTACCTTTGTTTAGCTGGTAACAAGCTCCTTGGGTGGTTCACTAAATACTTTTGGTATAGAAAAAGGTGTGTGTTTTTTAACCAGAACCATTTAAAACAAGGTATTTTAAAGATTAGTGTTTACTTTTTAAGTGCAAAAAGGTGTAAAAAAGTTTATGAAATCTTAAAAAATACCTTTCAAGAAAAGGTTAATGTTTTAAGTTTTTCTAATAATTTAATTGAGATAACTCATCATGATGCTAATAAGGGTTATGCAATTGAATATATGGCCAAAAGAGAACAACTTTCACTTAATAGAATAGCAGTTATTGGTGATTCTTGA", "aa": "MIDLLGLDLDGTLLSKTKKINNPSKLALTNLIAKKPSLKVMILTGRSVFSTLKHVEKLNSLFKKPIVDYFCCYGGAKLYQIEANKPQERYKFCLENSVVETTFSIIKKHRGLCLAYLDSYVSPYLCLAGNKLLGWFTKYFWYRKRCVFFNQNHLKQGILKISVYFLSAKRCKKVYEILKNTFQEKVNVLSFSNNLIEITHHDANKGYAIEYMAKREQLSLNRIAVIGDS", "aa_hexdigest": "4d2dd27c5f4598e07b4c9c74f5563782", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47371", "query_cov": 1.0, "subject_cov": 0.8035087719298246, "identity": 1.0, "score": 461.0, "evalue": 2.86e-163, "valid": true, "gene": "Cof", "product": "Putative phosphatase MG125", "ec_ids": [ "3.1.3.-" ], "uniref50_id": "UniRef50_P47371", "cog_id": "COG0561", "cog_category": "HR", "go_ids": [ "GO:0005829", "GO:0000287", "GO:0016791" ] }, "pscc": { "uniref50_id": "UniRef50_P47371", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47371" ], "product": "Putative phosphatase MG125" }, "genes": [ "cof" ], "id": "KMADJIBOEG_325", "locus": "GCA000292445_00286" }, { "type": "cds", "sequence": "contig_1", "start": 153750, "stop": 153905, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCAAAAAAGCTAAATATTCCTTTGCAATGTCAAAATCCCCTTCCCAGTTAAAATTAATTGCTACCAATACCAGTAACAAAACCAACCGTTACCGCTTTAGTACCTTACTTAATTTAATTAGTGAAACAATCATTAATCAAAAAGCTGATTAG", "aa": "MFKKAKYSFAMSKSPSQLKLIATNTSNKTNRYRFSTLLNLISETIINQKAD", "aa_hexdigest": "586c7c2a6f62f259930e18d13f136901", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5843.731199999998, "isoelectric_point": 10.29278049468994 }, "id": "KMADJIBOEG_326", "locus": "GCA000292445_00287" }, { "type": "cds", "sequence": "contig_1", "start": 153886, "stop": 154929, "strand": "-", "frame": 1, "gene": "trpS", "product": "tryptophan--tRNA ligase", "db_xrefs": [ "COG:COG0180", "COG:J", "EC:6.1.1.2", "GO:0000166", "GO:0004830", "GO:0005524", "GO:0005829", "GO:0006436", "RefSeq:WP_014893947.1", "SO:0001217", "UniParc:UPI00027B3541", "UniRef:UniRef100_UPI00027B3541", "UniRef:UniRef50_P75510", "UniRef:UniRef90_P47372" ], "nt": "ATGATAAAGCGCGCAATTACAGGGATTCAAGCTTCTGGAAGACAACACCTAGGTAACTTTCTTGGTGTAATGCAAGGTTTAAAACAACTCCAAAGTCAATACCAACTGTTTTTATTTGTTGCTGATCTTCATGCTATTACTGTTGATTTTGAACCAACAATGCTCAAAGATAACAACTTGCAACTTGTTAAAACTTTATTAGCACTAGGACTTGATTATGGAAAAGTGAACTTATTTTTACAAAGTGATCTGATGGAACATACCATGTTAGGTTATCTAATGCTGACACAAAGTAATCTAGGTGAATTACAAAGAATGACCCAATTTAAAACAAAGAAATTAGCGCAAAAAAGAAATAGTAATAACACCATTACTATCCCAACTGGTTTGTTAACTTACCCAGTGTTAATGGCTGCTGATATCTTGCTTTATCAACCTGATATTGTTCCAGTTGGTAATGATCAGAAGCAGCACTTGGAATTAACCAATGATTTAGCTAAACGTATAGCAAAAAAATTTAAGTTAAAACTGAAATTACCTGTATTTATAGAAAACAAAGATACCAACAGGATCATGGATCTATCAAATCCTTTAAAGAAGATGTCCAAATCAAATCCTGATCAAAATGGTGTTATCTATCTGGATGATAGTAAAGAAACAATCATCAAAAAAGTGCGCAAAGCCACAACTGATAGTTTTAATAAGATTCGTTTTGCTAAAAAAACCCAACCTGGTGTTACTAATTTACTTGTTATTTTAACTGCACTTTTAAAAGAAGAAGTTAACCATAATTTAAGTAAAAAAATCGGCTCTGATCTTGTTAAATATTATCAGAATAAAAGTTATTTAGATTTAAAGAATGACCTCAGTAGTGCTGTTATTAATGTCATAGAATCACTTAAATTTAAAAAAGCACAAATTACTGATGAAATGGTATTAAAAGTCCTAAATGATGGTAAAAACCAAGCTAAAAAAGTTGCTGATGAAACATTAAAAATGTTTTATAAAGCATTTGGTTTAACATCTAATCAGCTTTTTGATTAA", "aa": "MIKRAITGIQASGRQHLGNFLGVMQGLKQLQSQYQLFLFVADLHAITVDFEPTMLKDNNLQLVKTLLALGLDYGKVNLFLQSDLMEHTMLGYLMLTQSNLGELQRMTQFKTKKLAQKRNSNNTITIPTGLLTYPVLMAADILLYQPDIVPVGNDQKQHLELTNDLAKRIAKKFKLKLKLPVFIENKDTNRIMDLSNPLKKMSKSNPDQNGVIYLDDSKETIIKKVRKATTDSFNKIRFAKKTQPGVTNLLVILTALLKEEVNHNLSKKIGSDLVKYYQNKSYLDLKNDLSSAVINVIESLKFKKAQITDEMVLKVLNDGKNQAKKVADETLKMFYKAFGLTSNQLFD", "aa_hexdigest": "2cfd4403f99558215ca1a88b8e66bd60", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3541", "ncbi_nrp_id": "WP_014893947.1", "uniref100_id": "UniRef100_UPI00027B3541", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3541", "RefSeq:WP_014893947.1", "UniRef:UniRef100_UPI00027B3541" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3541", "uniref90_id": "UniRef90_P47372" }, "psc": { "uniref90_id": "UniRef90_P47372", "gene": "trpS", "product": "tryptophan--tRNA ligase", "ec_ids": [ "6.1.1.2" ], "uniref50_id": "UniRef50_P75510", "cog_id": "COG0180", "cog_category": "J", "go_ids": [ "GO:0000166", "GO:0004830", "GO:0005524", "GO:0005829", "GO:0006436" ] }, "pscc": { "uniref50_id": "UniRef50_P75510", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75510" ], "product": "Tryptophan--tRNA ligase" }, "genes": [ "trpS" ], "id": "KMADJIBOEG_327", "locus": "GCA000292445_00288" }, { "type": "cds", "sequence": "contig_1", "start": 154965, "stop": 155402, "strand": "+", "frame": 3, "gene": "arsC", "product": "putative protein MG127", "db_xrefs": [ "COG:COG1393", "COG:P", "GO:0016491", "KEGG:K16509", "RefSeq:WP_010869345.1", "SO:0001217", "UniParc:UPI0000139302", "UniRef:UniRef100_P47373", "UniRef:UniRef50_P75509", "UniRef:UniRef90_P47373" ], "nt": "ATGAAAAAAGCGGGTAAAAAAAATAGTGATGCAGGTAAGACTTTTATCTTAATCTCATCATCCTGTTCATCCTGTCAGAAAGCCATTGAGTTTTTTGATCAAAACAAAATTAGCTATGTTGTTGAGAATTTTTATAAAAAACCAATTAGTGATAAGCGTTTTAAAGATATTTTAAGTCTTAGTGAGGATGGTACTGAAAGTTTGTTTTCCAAACGTGCTGATCAGATTAAAGCAACTAACAGTGTTAGTGTTGAAGAGTTGAGTATCAGTGAATTAATTAAGCTAGTTAGAGAACGTCCTTCTTTACTAAGAAGGCCTATTATCATCCAATATAATTCTTCAGGAATTCCTAAAAGGATGCGAATTGGTTATAACTCATCTGAAATTAAGGTCTTTGAGCGTAAACTAATAGAACCAAAGCCTATAATACAACAATAA", "aa": "MKKAGKKNSDAGKTFILISSSCSSCQKAIEFFDQNKISYVVENFYKKPISDKRFKDILSLSEDGTESLFSKRADQIKATNSVSVEELSISELIKLVRERPSLLRRPIIIQYNSSGIPKRMRIGYNSSEIKVFERKLIEPKPIIQQ", "aa_hexdigest": "40e8ac7b4528c13aeb66ae30ddcdf3a3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139302", "ncbi_nrp_id": "WP_010869345.1", "uniref100_id": "UniRef100_P47373", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139302", "RefSeq:WP_010869345.1", "UniRef:UniRef100_P47373" ] }, "ips": { "uniref100_id": "UniRef100_P47373", "uniref90_id": "UniRef90_P47373" }, "psc": { "uniref90_id": "UniRef90_P47373", "gene": "ArsC", "product": "Uncharacterized protein MG127", "uniref50_id": "UniRef50_P75509", "cog_id": "COG1393", "cog_category": "P", "kegg_orthology_id": "K16509", "go_ids": [ "GO:0016491" ] }, "pscc": { "uniref50_id": "UniRef50_P75509", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75509" ], "product": "Uncharacterized protein MG127 homolog" }, "genes": [ "arsC" ], "id": "KMADJIBOEG_328", "locus": "GCA000292445_00289" }, { "type": "cds", "sequence": "contig_1", "start": 155410, "stop": 156189, "strand": "+", "frame": 1, "gene": "nadK", "product": "NAD(+) kinase", "db_xrefs": [ "COG:COG0061", "COG:H", "EC:2.7.1.23", "GO:0003951", "GO:0005524", "GO:0005737", "GO:0006741", "GO:0019674", "GO:0046872", "GO:0051287", "KEGG:K00858", "RefSeq:WP_014894158.1", "SO:0001217", "UniParc:UPI00027B3542", "UniRef:UniRef100_UPI00027B3542", "UniRef:UniRef50_P75508", "UniRef:UniRef90_P47374" ], "nt": "ATGAAATACAAAATTTTTGCTTCCACAACGCCCCAAACTGAACCTGTTCTCAATAAGTTAAGAACAGTTTTAAAAACTTGGCAAGCGGTTGAGAATGGTTATGAATATGTGTTTGTTTTAGGTGGGGATGGTTTTTTTGTTTCCACACTTGCTAACTATAACTGTGATAGTTGTAAGGTAGTTGGTATCAATACTGGCCACCTTGGTTTTTACACTTCTTTTAATGGAGATGATCTTGATGAAAATTTCATTTCAAAACTAACTAGCTTTGAGTTTAGAAAGATTAATTTACTGGAAGTGAAAACTGAAAACCACAGTTTCTTAGTTTTAAATGAACTTGCAGTTTACACCAACACTGCATATCCAATTAACATCTTTATTGATGATAACCCCTGGGAATCATACCGCGGTTCAGGGTTGCTAATTGGCCCAAGAACAGGCTCAACTGCCCTAGCAAAATCCGCTAAGGGAGCGGTTATTTTTCCAAATGTTGATGTTGTTCAAATTATTGAACTAAACCCCTTATTACATCCCAACCAAATCACAATTCAATCTCCGATTATTCTGCCAATGCAAACCAAGGTTGAATTTAGGATCAAAAAGGCTTTTAAAGCAGAGCAATTTCCCAATTTTTATGCAGATGGAATCAAGCTTGATTTAAAAAATGAAGATACCAGTATTAGCTTTCAGTTGGTTTTATCAAGATCAATGTTTCACGCTTCTTTAAAAACTAAGGATTTTATTGATAAACTAAAGTCAACTTTTATTAAGCAAAGCTAA", "aa": "MKYKIFASTTPQTEPVLNKLRTVLKTWQAVENGYEYVFVLGGDGFFVSTLANYNCDSCKVVGINTGHLGFYTSFNGDDLDENFISKLTSFEFRKINLLEVKTENHSFLVLNELAVYTNTAYPINIFIDDNPWESYRGSGLLIGPRTGSTALAKSAKGAVIFPNVDVVQIIELNPLLHPNQITIQSPIILPMQTKVEFRIKKAFKAEQFPNFYADGIKLDLKNEDTSISFQLVLSRSMFHASLKTKDFIDKLKSTFIKQS", "aa_hexdigest": "b6a04b8198a2fd4c1038b349981c046c", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3542", "ncbi_nrp_id": "WP_014894158.1", "uniref100_id": "UniRef100_UPI00027B3542", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3542", "RefSeq:WP_014894158.1", "UniRef:UniRef100_UPI00027B3542" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3542", "uniref90_id": "UniRef90_P47374" }, "psc": { "uniref90_id": "UniRef90_P47374", "gene": "nadK", "product": "NAD(+) kinase", "ec_ids": [ "2.7.1.23" ], "uniref50_id": "UniRef50_P75508", "cog_id": "COG0061", "cog_category": "H", "kegg_orthology_id": "K00858", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0046872", "GO:0051287", "GO:0003951", "GO:0019674", "GO:0006741" ] }, "pscc": { "uniref50_id": "UniRef50_P75508", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75508" ], "product": "NAD kinase" }, "genes": [ "nadK" ], "id": "KMADJIBOEG_329", "locus": "GCA000292445_00290" }, { "type": "cds", "sequence": "contig_1", "start": 156207, "stop": 156542, "strand": "+", "frame": 3, "gene": "ptsG2", "product": "Putative phosphotransferase enzyme IIB component MG129", "db_xrefs": [ "COG:COG1264", "COG:G", "EC:2.7.1.-", "GO:0008982", "GO:0009401", "GO:0016020", "SO:0001217", "UniRef:UniRef50_P75507", "UniRef:UniRef90_Q49397" ], "nt": "TTGGGTTATATTTTTAGTTTTGGTTTACTCTATCTTTGGATAGTAAAAAAATCAAAACAAATTGCTCAACAACCCAACACAAAACTGGTTGAATCAACTTCTATTCCTTTTAAAGTTAAGGACTTTGTTAGTGCTTGTGGTGGTAAGAAAAACTTTGTTAATATAAAAACAACACCAACTCAATTAATAGTTACTTTTAAAGATGTTAACTCAGTGAGCTTAACAAAGCTTAATGCGCTTAATATCAAAGGAATTAACAAAAACCAAAACCAATTTCGTTTTGTACTTGGTAACTTTGTAAATGAATTGAAAAAAAAGATAGAAGATGAACAATAA", "aa": "MGYIFSFGLLYLWIVKKSKQIAQQPNTKLVESTSIPFKVKDFVSACGGKKNFVNIKTTPTQLIVTFKDVNSVSLTKLNALNIKGINKNQNQFRFVLGNFVNELKKKIEDEQ", "aa_hexdigest": "0f26b1eea215da6edac1f2fc5c3e7254", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q49397", "query_cov": 1.0, "subject_cov": 0.9487179487179487, "identity": 0.982, "score": 214.0, "evalue": 2.41e-70, "valid": true, "gene": "PtsG2", "product": "Putative phosphotransferase enzyme IIB component MG129", "ec_ids": [ "2.7.1.-" ], "uniref50_id": "UniRef50_P75507", "cog_id": "COG1264", "cog_category": "G", "go_ids": [ "GO:0016020", "GO:0008982", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P75507", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75507" ], "product": "Putative phosphotransferase enzyme IIB component MPN_268" }, "genes": [ "ptsG2" ], "id": "KMADJIBOEG_330", "locus": "GCA000292445_00291" }, { "type": "cds", "sequence": "contig_1", "start": 156532, "stop": 157824, "strand": "+", "frame": 1, "gene": "rny", "product": "Ribonuclease Y", "db_xrefs": [ "COG:COG1418", "COG:JR", "EC:3.1.-.-", "GO:0003723", "GO:0004521", "GO:0005886", "GO:0006402", "SO:0001217", "UniRef:UniRef50_P47376", "UniRef:UniRef90_P47376" ], "nt": "ATGAACAATAACATTACTAACAGTATTGTCCAATTGTTTTTCAACACTTCTCTCTTTGCTTTTCTGTTTTTAATCATTATTGCGTTTAATTTATGCTTGTTTGCCTATCTTTATTTTCAGTACCGAATTTATAAGAAAAACCCTAAAAAAGCTAACAACTTTAAAGCGAATGAATATGAAAAAATTAAGCTATTAAAAAACCAAAATTTCACTGAAAGTAATAAATTAATTGCAACAACTAATGAGTTAAATGAACTTACTAGTCAACTAGATAATATCTTGGTTAGGATTATCAACAAACCACTAGCAAAGTTAGTTAATGATTTTTTAGATGAACAGATTAAACAGATAGTTAAGCTAGATAAAAACAGTTCTGATTTTCACTCAGAAAGTGATAATCTCCCTTTTTATACCAAACTCTTTAATGATTTTCACTTTGGTGTTGATAAATTAATAAACATTAACATAAAAAACCCTCTTTATAACTGGGTTTATAGCCCCAGTTTTTTAATTAGTGAAAGTGATTTTCGCAAGCTTAATGGTATTAGTGGTATCAATAAAAAGCTTTTGGTTGAAAAACTTAGAATTGAAGACATTGTGTTTACAGATCTCAACAAAAAGTATGAAGTTAATGTCTTGACAGAAAGTCCTATTAAAGCACAAAAAACAGTGTTAACTGTGCGTAATATCCTGATGAACGATTATGTTGATAATGAAAGGATTGAATCATATGTCCAACAAGCTAACTTCTTTTTTACTGAGCACTGTAAAAAGATCGGTAAAGAGATCTTAGAATCACTTAATATTTTTATCTCAAGTAGTTCACTACACCGTCATTTTGGCTTTTTAGCATTTCGCTATTCATTTGGACAAAATGTCTTATCCCATAGCCTTGAAACTGCATTTTTAACTGCCCACTTAGCAGCTTTAATAGAACTTGATAGTGAACTGAGTTTAAAGTGTGGATTGCTCCATGATATTGGTAAATCTAATGATGATAATGGTAAAGAGAGCCATACGATTACAGGCGCTAAATTCGCTGAGCAATTTCAACTACCTGATGACATTAAATACACAATTGCTAACCACCACAATAAACATATTGACAATACTTATTGTCGTTTAACACAAATTGCTGATAAACTATCTGCTGCTAGAATTGGTGCTAGAAGTGATAGTTCGCTTCTTTTTAAACAACTAAAAGATGAGTTGAAAAAGATTGTTGATAAAACTATTAATAATTTTCATACAACGATCTTACTAGGTCAAAGTGGTAGAAGGTTAATGATTTGA", "aa": "MNNNITNSIVQLFFNTSLFAFLFLIIIAFNLCLFAYLYFQYRIYKKNPKKANNFKANEYEKIKLLKNQNFTESNKLIATTNELNELTSQLDNILVRIINKPLAKLVNDFLDEQIKQIVKLDKNSSDFHSESDNLPFYTKLFNDFHFGVDKLININIKNPLYNWVYSPSFLISESDFRKLNGISGINKKLLVEKLRIEDIVFTDLNKKYEVNVLTESPIKAQKTVLTVRNILMNDYVDNERIESYVQQANFFFTEHCKKIGKEILESLNIFISSSSLHRHFGFLAFRYSFGQNVLSHSLETAFLTAHLAALIELDSELSLKCGLLHDIGKSNDDNGKESHTITGAKFAEQFQLPDDIKYTIANHHNKHIDNTYCRLTQIADKLSAARIGARSDSSLLFKQLKDELKKIVDKTINNFHTTILLGQSGRRLMI", "aa_hexdigest": "a175b57091d63aa77f674ff23c7434ea", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47376", "query_cov": 1.0, "subject_cov": 0.8884297520661157, "identity": 0.993, "score": 828.0, "evalue": 4.68e-302, "valid": true, "gene": "rny", "product": "Ribonuclease Y", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P47376", "cog_id": "COG1418", "cog_category": "JR", "go_ids": [ "GO:0005886", "GO:0003723", "GO:0004521", "GO:0006402" ] }, "pscc": { "uniref50_id": "UniRef50_P47376", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47376" ], "product": "Ribonuclease Y" }, "genes": [ "rny" ], "id": "KMADJIBOEG_331", "locus": "GCA000292445_00292" }, { "type": "cds", "sequence": "contig_1", "start": 157876, "stop": 157986, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGAAAAGATTAAAGCTGAAATTGCTAAGAATTCAATTACAAATCACTTCCCTATTAAAGTTGTAATTAGATATAACTTTGAACATAGTTTCAACACCAAAAGCTAA", "aa": "MVEKIKAEIAKNSITNHFPIKVVIRYNFEHSFNTKS", "aa_hexdigest": "5711583dc453818723a3f5b9a9c8542d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4234.875800000001, "isoelectric_point": 9.698124122619632 }, "id": "KMADJIBOEG_332", "locus": "GCA000292445_00293" }, { "type": "cds", "sequence": "contig_1", "start": 157989, "stop": 158276, "strand": "+", "frame": 3, "gene": null, "product": "MPN270 family protein", "db_xrefs": [ "RefSeq:WP_014894161.1", "SO:0001217", "UniParc:UPI00027B3545", "UniRef:UniRef100_UPI00027B3545", "UniRef:UniRef50_Q9EXD1", "UniRef:UniRef90_UPI00027B3AA2" ], "nt": "ATGCAATACAGCGCTTTAATACCACTTTTTATATTACTAATTAGCTTAGTATTATTTTGTTTCAGCTTTAGAAAAAATCAAAGTGAAAATCAGATAGTAAAGATCTTATTTTTTGCTTATTGCATCGATTTTTTAGCTTTAATTCTTGCTGTAATGTTGCTCACTTTTTTAAGTCATGGGTTGTTAAGTTTAGCGATTTTAATCCCTGTCTTAGTTTTTTCAATAATAATGTTTTTTGTTATGGTTATTAGTCATTATCCCCTTATGAAAAGACTATTTGGAAATTAA", "aa": "MQYSALIPLFILLISLVLFCFSFRKNQSENQIVKILFFAYCIDFLALILAVMLLTFLSHGLLSLAILIPVLVFSIIMFFVMVISHYPLMKRLFGN", "aa_hexdigest": "5313ef91cfd34330cb74841903839d62", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3545", "ncbi_nrp_id": "WP_014894161.1", "uniref100_id": "UniRef100_UPI00027B3545", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3545", "RefSeq:WP_014894161.1", "UniRef:UniRef100_UPI00027B3545" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3545", "uniref90_id": "UniRef90_UPI00027B3AA2" }, "psc": { "uniref90_id": "UniRef90_UPI00027B3AA2", "product": "MPN270 family protein", "uniref50_id": "UniRef50_Q9EXD1" }, "pscc": { "uniref50_id": "UniRef50_Q9EXD1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9EXD1" ], "product": "Uncharacterized protein MG131 homolog" }, "genes": [], "id": "KMADJIBOEG_333", "locus": "GCA000292445_00294" }, { "type": "cds", "sequence": "contig_1", "start": 158290, "stop": 158571, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_014894579.1", "SO:0001217", "UniParc:UPI00027B3546", "UniRef:UniRef100_UPI00027B3546", "UniRef:UniRef50_UPI00027B3546", "UniRef:UniRef90_UPI00027B3546" ], "nt": "ATGGAGTTGAAAACCCCTAATTTTAAGCTAATTGATGAAAAGATTGCTGAATTTAATAAGAGCAATGAAAACCTGATTGTAAAACTACTTCAAAAAGAAAAGGAATTTGCCACAAACCAAGTTACTATTCAGTTTGATACTCAGTCAAAAAAGTCAGAAGAAGTGAAAAAACCTAGTAAAAAAATACTGAAAAATTATCACTTTCTAACAGTAGTTATGGCAGTTAATTTGCTAATAACTGTTGGTTTGATAGTTGGTTTAATTTTTGCTTTCAGATATTAG", "aa": "MELKTPNFKLIDEKIAEFNKSNENLIVKLLQKEKEFATNQVTIQFDTQSKKSEEVKKPSKKILKNYHFLTVVMAVNLLITVGLIVGLIFAFRY", "aa_hexdigest": "9902819cd6e11ed9730a20e2170c00a4", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3546", "ncbi_nrp_id": "WP_014894579.1", "uniref100_id": "UniRef100_UPI00027B3546", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3546", "RefSeq:WP_014894579.1", "UniRef:UniRef100_UPI00027B3546" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3546", "uniref90_id": "UniRef90_UPI00027B3546" }, "psc": { "uniref90_id": "UniRef90_UPI00027B3546", "uniref50_id": "UniRef50_UPI00027B3546" }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3546", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3546" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10789.648899999993, "isoelectric_point": 9.52289905548096 }, "id": "KMADJIBOEG_334", "locus": "GCA000292445_00295" }, { "type": "cds", "sequence": "contig_1", "start": 158548, "stop": 158838, "strand": "-", "frame": 1, "gene": "hinT", "product": "putative HIT-like protein MG132", "db_xrefs": [ "COG:COG0537", "COG:FR", "GO:0003824", "GO:0009117", "SO:0001217", "UniRef:UniRef50_P47378", "UniRef:UniRef90_P47378" ], "nt": "GTGATCCCTAAAAAACATGCAGCTAATTTTTCTTCAACTGATCAAAAGGAGTTACAAGCAGTTAGTTTATTAGCAAAACAAATCGCTTTAAAACTAAAGATGACACTAAAACCATCTGGTTTAAACTATATTTCTAATGAAGGGGCAATTGCAGGTCAAGTGGTTTTTCATTTTCACTTACACATAGTACCCAAATACGAAACTGGTAAAGGGTTTGGCTATAATGTCAATAAAACCAACAAACGTTCCTTAGAAGAAAACTACCAACTAATATCTGAAAGCAAAAATTAA", "aa": "MIPKKHAANFSSTDQKELQAVSLLAKQIALKLKMTLKPSGLNYISNEGAIAGQVVFHFHLHIVPKYETGKGFGYNVNKTNKRSLEENYQLISESKN", "aa_hexdigest": "b0af0ab96ad53186677969373972de85", "start_type": "GTG", "rbs_motif": null, "genes": [ "hinT" ], "truncated": "3-prime", "pseudogene": { "start": 158548, "stop": 158973, "observations": { "mutation": [ 158922 ], "stop": [ 158922 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47378", "query_cov": 1.0, "subject_cov": 0.6808510638297872, "identity": 0.958, "score": 185.0, "evalue": 7.7e-59, "gene_start": 1, "gene_end": 96, "reference_start": 46, "reference_end": 141, "reference_sequence": "MEKNTTSSCIFCDIVQGSITSYKIGENEHAIAFLDAFPVADGHTLVIPKKHAVDFSSTDQKELQAVSLLAKQIALKLKMTLKPSGLNYVSNEGAIAGQVVFHFHLHIVPKYETGKGFGYNVNKTNKRSLEENYQLISESKN" }, "paralog": false, "identity": 0.9716312056737588, "score": 276.0, "evalue": 2.44e-97, "description": "Internal stop codon at 158922. Nonsense mutation around 158922.", "psc": { "uniref90_id": "UniRef90_P47378", "gene": "HinT", "product": "Uncharacterized HIT-like protein MG132", "uniref50_id": "UniRef50_P47378", "cog_id": "COG0537", "cog_category": "FR", "go_ids": [ "GO:0003824", "GO:0009117" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47378", "UniRef:UniRef50_P47378", "COG:COG0537", "COG:FR", "GO:0003824", "GO:0009117" ] }, "pscc": { "uniref50_id": "UniRef50_P47378", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47378" ], "product": "Uncharacterized HIT-like protein MG132" } }, "id": "KMADJIBOEG_335", "locus": "GCA000292445_00296" }, { "type": "cds", "sequence": "contig_1", "start": 159521, "stop": 159745, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTAAAAAATCAATTGGAATTTTTTACCGTTGCTTTTATCTTAACAATAAATGCGATTATTATTTAATTTTTCTAGCACCTTTTTCACTATTCACCCAAATCTTTATGGTGATTACTGCACTGATTAGTGTTGCTAACAGTGGTCAAATGAGCCTGATTTGGTTTACTAATTTTGATACTTTTACTTACCAAAGTAATAGCCTTGCAATCTTTTTAGTATGA", "aa": "MLKKSIGIFYRCFYLNNKCDYYLIFLAPFSLFTQIFMVITALISVANSGQMSLIWFTNFDTFTYQSNSLAIFLV", "aa_hexdigest": "90d20f24e012c007cd41cdfb7436b8e3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8649.143199999995, "isoelectric_point": 8.683907508850098 }, "id": "KMADJIBOEG_336", "locus": "GCA000292445_00297" }, { "type": "cds", "sequence": "contig_1", "start": 159765, "stop": 160067, "strand": "+", "frame": 3, "gene": "ybaB", "product": "YbaB/EbfC family nucleoid-associated protein", "db_xrefs": [ "COG:COG0718", "COG:K", "GO:0003677", "KEGG:K09747", "RefSeq:WP_010869347.1", "SO:0001217", "UniParc:UPI000013932E", "UniRef:UniRef100_P47380", "UniRef:UniRef50_P47380", "UniRef:UniRef90_P47380" ], "nt": "ATGAGTTTTAAAAAGATTGCTGAAATGATGCGTCAAGCAGAACGAGAAACTAAGAAAAAAACATTAGCATTTGAACAACAAGCCTTTGAATACAACTATAAAAATGGTGCGATTAAGATCACTATTTTAGGTGATCTTACACTTAAATCAATTAACATCGATCCTGTTTTGATTGATGCAAGCGACAAAGTTATTCTAGAGGAGATGATTATAGAAGCTACTAATGAAGCGGTTAGTGATGTGAAAACTAAGTATGATAACTTAGTTGAGAAAACTATGCCAAAAGTTCCAGGTCTTTTCTAA", "aa": "MSFKKIAEMMRQAERETKKKTLAFEQQAFEYNYKNGAIKITILGDLTLKSINIDPVLIDASDKVILEEMIIEATNEAVSDVKTKYDNLVEKTMPKVPGLF", "aa_hexdigest": "c413e76280bd3195594ee858ba01ca99", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013932E", "ncbi_nrp_id": "WP_010869347.1", "uniref100_id": "UniRef100_P47380", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013932E", "RefSeq:WP_010869347.1", "UniRef:UniRef100_P47380" ] }, "ips": { "uniref100_id": "UniRef100_P47380", "uniref90_id": "UniRef90_P47380" }, "psc": { "uniref90_id": "UniRef90_P47380", "gene": "YbaB", "product": "YbaB/EbfC family nucleoid-associated protein", "uniref50_id": "UniRef50_P47380", "cog_id": "COG0718", "cog_category": "K", "kegg_orthology_id": "K09747", "go_ids": [ "GO:0003677" ] }, "pscc": { "uniref50_id": "UniRef50_P47380", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47380" ], "product": "Uncharacterized protein MG134" }, "genes": [ "ybaB" ], "id": "KMADJIBOEG_337", "locus": "GCA000292445_00298" }, { "type": "cds", "sequence": "contig_1", "start": 160039, "stop": 160230, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCGACCTTTCTTAACTTAAGCAATAGTGATCCACAACTTGTGTTTATTGTGGGCTTTAGTTTAAGTTATGTTGCGATTTTACTAATGACACTCTTTGCTAATATCCCTACATTTATGGAAATGAAAAAAGATAAGATCTTTATTAAAAAGGAAAATCTAATTAGAAAAGACCTGGAACTTTTGGCATAG", "aa": "MATFLNLSNSDPQLVFIVGFSLSYVAILLMTLFANIPTFMEMKKDKIFIKKENLIRKDLELLA", "aa_hexdigest": "a115bad6ad2a63a293ff095ede57b6f9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7239.664999999997, "isoelectric_point": 8.064559745788571 }, "id": "KMADJIBOEG_338", "locus": "GCA000292445_00299" }, { "type": "cds", "sequence": "contig_1", "start": 160252, "stop": 160881, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MG135", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47381", "UniRef:UniRef90_P47381" ], "nt": "ATGCAGACACTTAACTACCTTGTCATCATCCTTATTATTGCTGGTGTAATTTCTGTTTTAGCTTTTACTCCTTTAGTTCGAAAACTAAAGATTAGATTCTATCTAATTCAAGCACTAGCCATTGTCCTGTTTGTCTATGTTTTTTTTGGTAGGCAGATTATTTATTTATTCCCTGATGTTTATGGACAAAATTCCCAATCTTCACAAACCTTAAATTCACTACGACTAAGTCGGATATTTTTATTAGATCTTTGTCCATTTTTTGCTGTTATTGCGCCAGTGTTTGTCTTTTTAAAGCAAAAAAAGATTAGTGGGGTGTTAGCTGTTTTTGGGTTATTTGGAGCTTTGGTTACTCTTTTTGGTGAACTAATTTTTACACCTGTTAATGAACAAGATATTGTCAATTTCATCTTTGTTGGTACAGGAAATAACCAGATTTATTTCATGATGCATTTTCTCAGTTTACTTGTAAGCTTAGCAATTATCTTGTGGGATAACTGTTTTTCTTTAATCTCATTTTTCTATATCCATGTGTTTGCTCTAATTTACTTTAGTTATGTAGCTTTGATGGTGTCTGTGTTTAAGGACCAGATCACAGGTAATACAACTGGTATCTTAGCAAGTGATTGA", "aa": "MQTLNYLVIILIIAGVISVLAFTPLVRKLKIRFYLIQALAIVLFVYVFFGRQIIYLFPDVYGQNSQSSQTLNSLRLSRIFLLDLCPFFAVIAPVFVFLKQKKISGVLAVFGLFGALVTLFGELIFTPVNEQDIVNFIFVGTGNNQIYFMMHFLSLLVSLAIILWDNCFSLISFFYIHVFALIYFSYVALMVSVFKDQITGNTTGILASD", "aa_hexdigest": "9dc286f51cf163c314bd787bf31237e9", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 160039, "stop": 160881, "observations": { "mutation": [ 160254 ], "stop": [ 160254 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47381", "query_cov": 1.0, "subject_cov": 0.7464285714285714, "identity": 0.981, "score": 377.0, "evalue": 1.72e-130, "gene_start": 1, "gene_end": 209, "reference_start": 1, "reference_end": 209, "reference_sequence": "MQTLNYLVIILIIAGVISVLAFTPLVRKLKIRFYLIQVLAIVLFVYVFFGRQIIYLFPDVYGQNSQSSQNLDSLRLSRIFLLDLCPFFAVIAPVFVFLKQKKISGVLAVFGLFGALVTLFGELIFTPVNEQDIVNFIFVGTGNNQIYFMMHFLSLLVSLAIILWDNCFSLISFFYIHVFALIYFSYVALMVSVFKGQITGNTTGILASDWTNGEYKNVATFLNLSNSDPQLVFIVGFSLSYVAILLMTLFANIPTFMEMKKDKIFIKKENLIRKDLELLA" }, "paralog": false, "identity": 0.9821428571428571, "score": 529.0, "evalue": 5.51e-193, "description": "Internal stop codon at 160254. Nonsense mutation around 160254.", "psc": { "uniref90_id": "UniRef90_P47381", "product": "Uncharacterized protein MG135", "uniref50_id": "UniRef50_P47381", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47381", "UniRef:UniRef50_P47381", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47381", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47381" ], "product": "Uncharacterized protein MG135" } }, "id": "KMADJIBOEG_339", "locus": "GCA000292445_00300" }, { "type": "cds", "sequence": "contig_1", "start": 160930, "stop": 161358, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01336.31" ], "nt": "ATGAGTGATCGTTTAAATGATCAAGCCCAACATCGCTTGCAGAAACTTTTAAGGTTAAAACAAACTAATAATGACCCTTATTTAGTAACAAAAACTAGTCTAACCCATTCTTCAAAAAGCTTTCAAGTTGAATTTGAAAAATGTGCAGAAGAAGAGTTGAAGAAAAAAACAACTGTCTCACTAGCTGGAAGGATCATTGCTATTAGACAAACCTTTTTAATTATTCAAGATTTTGATGGTCAAGTCCAACTTTACATCAATAAAAAAATCCATCCTAAGTTATTTGATTACTTTAATGAATTTGTTGATATTGGTGATCAAATTGTTGTTAGTGGTAAGCCAATGTTAACTAAAACAAAGGTATTAACTTTAGCTGTTGAAGAGATGAAAATCATTGCTAAGTGTTTATTGGTTCCACCTGAAAAGTGA", "aa": "MSDRLNDQAQHRLQKLLRLKQTNNDPYLVTKTSLTHSSKSFQVEFEKCAEEELKKKTTVSLAGRIIAIRQTFLIIQDFDGQVQLYINKKIHPKLFDYFNEFVDIGDQIVVSGKPMLTKTKVLTLAVEEMKIIAKCLLVPPEK", "aa_hexdigest": "aa2449725f59e95f0ce925ffb6d482a0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01336.31", "name": "tRNA_anti-codon", "length": 79, "aa_cov": 0.5211267605633803, "hmm_cov": 0.9493670886075949, "evalue": 2.2289116942903997e-13, "score": 45.133270263671875, "start": 59, "stop": 132 } ], "seq_stats": { "molecular_weight": 16363.982499999995, "isoelectric_point": 9.205779075622559 }, "id": "KMADJIBOEG_340", "locus": "GCA000292445_00301" }, { "type": "cds", "sequence": "contig_1", "start": 161425, "stop": 161823, "strand": "+", "frame": 1, "gene": null, "product": "Lysine--tRNA ligase, heat inducible", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X2LQM9" ], "nt": "ATGCGTGATGTTTTTCTGAAACGCACTAAGATTATTAAATCAATCCGTAGCTTTCTTGATCAAAATGGTTTTATTGAGGTTGAAACCCCCACTTTACAAGCTGTTTTAGGAGGAGCTAATGCTAAACCCTTTAAAACCCATTACAATGCTTTAAAAGCGGATTTTTATCTCAGAATTGCTAATGAAATAGCATTAAAAAAACTCATTATTGGTGGATTTAATAAGGTTTATGAAATGGGTAAAATGTTCCGCAATGAAGGGGTTGATACTACCCACAATCCTGAGTTTACCAGTATTGAAATATATCAAGCTTATGCAGATTTTGAAGTCATGCTTGTGCTTGTTGAAAAGATGATTCAATCACTTTGTGAAAGCTTAAACCAATTTAGCTTTAACTGA", "aa": "MRDVFLKRTKIIKSIRSFLDQNGFIEVETPTLQAVLGGANAKPFKTHYNALKADFYLRIANEIALKKLIIGGFNKVYEMGKMFRNEGVDTTHNPEFTSIEIYQAYADFEVMLVLVEKMIQSLCESLNQFSFN", "aa_hexdigest": "1829c73eaefdb21b6434c19b0d7d8a0c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2X2LQM9", "query_cov": 0.9242424242424242, "subject_cov": 0.8652482269503546, "identity": 0.5489999999999999, "score": 149.0, "evalue": 4.7e-44, "valid": false, "product": "Lysine--tRNA ligase, heat inducible", "uniref50_id": "UniRef50_A0A2X2LQM9" }, "pscc": { "uniref50_id": "UniRef50_A0A2X2LQM9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X2LQM9" ], "product": "Lysine--tRNA ligase, heat inducible" }, "genes": [], "id": "KMADJIBOEG_341", "locus": "GCA000292445_00302" }, { "type": "cds", "sequence": "contig_1", "start": 161869, "stop": 162402, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGGTTGAACTTATTAAGAAAGTTACAGGGATCGATTTTAATTCAGTAAAAGATGATCAATCTGCCATTTTATTAGCAGAAAAACACCATGTTAAACTAGCAAAACACCAACAAAATAAGCAACACATCATTAATTTGTTTTTTGAACAATTTTGTGAACAAACATTAATTGAACCTACCTTTGTAACCCATTATCCAAAAGCAGTTTCTCCTCTAGCAAAACAAGATCCTTCAAATCCTGAATTCACCCAACGATTTGAACTTTTTATTAATGGTAAAGAGATTGCTAATGCTTACAGCGAGCTAAACGATCCTTTAGAACAAAGAAAAAGGTTTGAACAACAACTTGAAGAAAAACAGCTTGGTAATGATGAGACAAGTGAACTTGATGAATCGTTTTTAGAAGCATTAAGTTTTGGGATGGTAAACACTGCTGGGCTTGGGATAGGTATTGATCGTTTGGTAATGTTGTTATGTGAATGTAATTCTATCCGTGATGTTGTTTTCTTCCCCCAGTTACGTGAACATAAATAG", "aa": "MVELIKKVTGIDFNSVKDDQSAILLAEKHHVKLAKHQQNKQHIINLFFEQFCEQTLIEPTFVTHYPKAVSPLAKQDPSNPEFTQRFELFINGKEIANAYSELNDPLEQRKRFEQQLEEKQLGNDETSELDESFLEALSFGMVNTAGLGIGIDRLVMLLCECNSIRDVVFFPQLREHK", "aa_hexdigest": "e535823dc2549131097509eb98553db5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0031D0E1C7", "query_cov": 0.9830508474576272, "subject_cov": 0.8093023255813954, "identity": 0.546, "score": 182.0, "evalue": 2.26e-55, "valid": false, "product": "amino acid--tRNA ligase-related protein", "ec_ids": [ "6.1.1.6" ], "uniref50_id": "UniRef50_A0A349PV97", "kegg_orthology_id": "K04567" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 127, "aa_cov": 0.6892655367231638, "hmm_cov": 0.968503937007874, "evalue": 1.5080334420319427e-37, "score": 124.41230773925781, "start": 52, "stop": 173 } ], "seq_stats": { "molecular_weight": 20391.9711, "isoelectric_point": 5.15656986236572 }, "id": "KMADJIBOEG_342", "locus": "GCA000292445_00303" }, { "type": "cds", "sequence": "contig_1", "start": 162365, "stop": 163204, "strand": "+", "frame": 2, "gene": "glf", "product": "UDP-galactopyranose mutase", "db_xrefs": [ "COG:COG0562", "COG:M", "EC:5.4.99.9", "GO:0005829", "GO:0008767", "GO:0009273", "GO:0050660", "KEGG:K01854", "SO:0001217", "UniRef:UniRef50_P75499", "UniRef:UniRef90_Q49398" ], "nt": "ATGTTGTTTTCTTCCCCCAGTTACGTGAACATAAATAGTTTTGATATTTTAATTGTTGGTGCTGGTATTAGTGGAATAGTACTAGCTAACATCTTAGCTAACCACAATAAAAGGGTTTTAATTGTTGAAAAAAGAGATCATATTGGTGGTAACTGTTATGATAAAGTTGATAGTAAAACTCAACTCTTGTTTCACCAGTATGGACCCCATATTTTCCATACTAACAACCAAACTGTTATTAACTTTATCTCACCCTTCTTTGAACTAAATAACTACCACCATCGGGTTGGTTTAAAATTGAAAAATAACCTAGATTTAACCTTACCCTTTGATTTTCAACAGATCTATAAACTAATGGGAAAAGATGGTAGAAAACTCGTTAGTTTTTTTAAAGAAAATTTCAGTTTAAATAGTCATCTATCATTAGCAGAATTACAACTAATTGATAATCCTTTAGCACAAAAACTCTATCAGTTTTTAATTAGTAATGTTTATAAACCATACAGTGTCAAAATGTGGGGTTTACCATTTGCAATGATTAATGAGAATGTTATTAACAGGGTCAAGATAGTTTTAAGTGAACAAAGCAGTTATTTTCCTGATGCAATTATCCAGGGATTACCTAAATCAGGTTATACAAACAGTTTTCTTAAGATGTTAGCCAATCCCTTAATTGATGTGCAGTTAAACTGCAAAGATAACCTTTTAGTTTATCAAGATGAAAAACTGTTTTTTAACAATACTTTAATAGAAAAACCAGTTGTTTACTGTGGTTTAATTGACAAGTTATTTAACTTTTGCTTTGGTCATTTGCAATACCGTTCCCTTGCCTTTAGTTGA", "aa": "MLFSSPSYVNINSFDILIVGAGISGIVLANILANHNKRVLIVEKRDHIGGNCYDKVDSKTQLLFHQYGPHIFHTNNQTVINFISPFFELNNYHHRVGLKLKNNLDLTLPFDFQQIYKLMGKDGRKLVSFFKENFSLNSHLSLAELQLIDNPLAQKLYQFLISNVYKPYSVKMWGLPFAMINENVINRVKIVLSEQSSYFPDAIIQGLPKSGYTNSFLKMLANPLIDVQLNCKDNLLVYQDEKLFFNNTLIEKPVVYCGLIDKLFNFCFGHLQYRSLAFS", "aa_hexdigest": "61f97aacc7777409a662d1c6cccc3038", "start_type": "ATG", "rbs_motif": null, "genes": [ "glf" ], "truncated": "3-prime", "pseudogene": { "start": 162344, "stop": 163507, "observations": { "mutation": [ 163202, 163436 ], "stop": [ 163202, 163436 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49398", "query_cov": 1.0, "subject_cov": 0.6905940594059405, "identity": 0.9890000000000001, "score": 557.0, "evalue": 1.33e-198, "gene_start": 1, "gene_end": 279, "reference_start": 8, "reference_end": 286, "reference_sequence": "MNVILSVMLFSSPSCVNINSFDILIVGAGISGIVLANILANHNKRVLIVEKRDHIGGNCYDKVDSKTQLLFHQYGPHIFHTNNQTVINFISPFFELNNYHHRVGLKLKNNLDLTLPFDFQQIYKLMGKDGRKLVSFFKENFSLNTHLSLAELQLIDNPLAQKLYQFLISNVYKPYSVKMWGLPFAMINENVINRVKIVLSEQSSYFPDAIIQGLPKSGYTNSFLKMLANPLIDVQLNCKDNLLVYQDEKLFFNNNLIEKPVVYCGLIDKLFNFCFGHLQYRSLAFSWKRFNQKKYQTYPVVNMPLAKSITRSVEYKQLTNQGSFKPQTIVSFETPGSYAINDPRFNEPYYPINNTLNDTLFKKYWKKASKLKNLHLLGRLATYQYIDMDKAILLSIKKAQQLLS" }, "paralog": false, "identity": 0.9844961240310077, "score": 769.0, "evalue": 1.16e-284, "description": "Internal stop codon at 163202, 163436. Nonsense mutation around 163202, 163436.", "psc": { "uniref90_id": "UniRef90_Q49398", "gene": "glf", "product": "UDP-galactopyranose mutase", "ec_ids": [ "5.4.99.9" ], "uniref50_id": "UniRef50_P75499", "cog_id": "COG0562", "cog_category": "M", "kegg_orthology_id": "K01854", "go_ids": [ "GO:0005829", "GO:0008767", "GO:0009273", "GO:0050660" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49398", "EC:5.4.99.9", "UniRef:UniRef50_P75499", "COG:COG0562", "COG:M", "KEGG:K01854", "GO:0005829", "GO:0008767", "GO:0009273", "GO:0050660" ] }, "pscc": { "uniref50_id": "UniRef50_P75499", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75499" ], "product": "UDP-galactopyranose mutase" } }, "id": "KMADJIBOEG_343", "locus": "GCA000292445_00304" }, { "type": "cds", "sequence": "contig_1", "start": 163250, "stop": 163438, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTTTAGCTAAATCAATCACAAGGAGTGTGGAATACAAACAACTAACAAACCAAGGTTCTTTCAAACCGCAAACCATCGTTAGTTTTGAAACCCCTGGCAGCTATGCAATTAACGATCCTAGGTTTAATGAACCTTATTACCCAATTAACAATACACTAAATGATACTCTTTTTAAAAAGTACTGA", "aa": "MPLAKSITRSVEYKQLTNQGSFKPQTIVSFETPGSYAINDPRFNEPYYPINNTLNDTLFKKY", "aa_hexdigest": "6b8a697082f073d04bad490dc692dfc9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7178.007700000001, "isoelectric_point": 9.04899196624756 }, "id": "KMADJIBOEG_344", "locus": "GCA000292445_00305" }, { "type": "cds", "sequence": "contig_1", "start": 163558, "stop": 165354, "strand": "+", "frame": 1, "gene": "lepA", "product": "translation elongation factor 4", "db_xrefs": [ "COG:COG0481", "COG:J", "EC:3.6.5.n1", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005886", "GO:0043022", "GO:0045727", "GO:1990606", "RefSeq:WP_014894168.1", "SO:0001217", "UniParc:UPI00027B3987", "UniRef:UniRef100_UPI00027B3987", "UniRef:UniRef50_B1X3K4", "UniRef:UniRef90_P47384" ], "nt": "ATGGAACAAAAAAACATTAGAAATTTTTCTATTATTGCCCATATTGATCATGGTAAATCTACCTTATCAGACCGCTTGTTAGAACATAGTTTAGGCTTTGAAAAAAGACTATTACAAGCGCAAATGCTTGATACTATGGAGATTGAAAGAGAAAGGGGTATTACCATTAAATTAAATGCTGTTGAGTTGAAAATTAATGTTGATAACAATAACTATCTTTTTCATTTAATTGACACCCCTGGGCATGTTGATTTTACTTATGAAGTGTCTCGTTCTTTAGCAGCTTGTGAGGGAGTTTTATTGTTAGTAGATGCAACCCAAGGAATTCAAGCACAAACGATTTCCAATGCCTATCTTGCGTTGGAAAATAACCTGGAAATTATCCCAGTCATTAACAAGATAGATATGGATAATGCTGATATTGAAACAACAAAAGATTCACTCCATAACCTATTAGGAGTTGAAAAAAACAGTATCTGTTTAGTATCTGCAAAAGCTAACTTAGGGATTGATCAGTTAATTCAAACAATTATAGCTAAGATCCCTCCACCAAAAGGAGAAATTAATAAACCTTTAAAAGCATTACTCTTTGATAGTTACTATGACCCTTACAAGGGGGTTGTTTGTTTTATTAGAGTATTTGATGGTTGTTTGAAGGTTAATGATAAGGTTCGTTTTATTAAAAGTAATTCTGTTTACCAAATTGTGGAACTAGGGGTTAAAACCCCATTTTTTGAAAAAAGAGATCAATTGCAAGCAGGGGATGTTGGTTGGTTTTCAGCAGGGATAAAAAAACTCCGTGATGTTGGGGTTGGTGATACTATTGTGAGTTTTGACGATCAATTTACAAAACCCTTAGCAGGTTATAAAAAGATCTTACCCATGATCTATTGTGGTTTATATCCAGTTGATAACAGTGATTATCAAAACCTCAAGTTAGCGATGAAAAAGATCATAATCAGTGATGCAGCATTGGAATATGAATATGAAACATCCCAAGCGTTAGGGTTTGGGGTTAGGTGTGGTTTTCTAGGTCTTTTACATATGGATGTTATTAAAGAAAGATTGGAAAGAGAATACAACCTAAAACTCATCTCAGCTCCCCCTTCAGTTGTATATAAGGTATTGTTAACCAATGGTAAAGAGATTAGTATTGATAATCCCTCTTTGTTACCAGAACGCTCCAAGATTAAAGCAATCAGTGAACCATTTGTAAAAGTTTTTATTGATTCACCTGATCAATATTTGGGCAGTGTTATTGATTTGTGCCAAAACTTCAGGGGTCAATATGAAAGTTTAAATGAGATTGATATCAACAGAAAAAGGATCTGTTATCTGATGCCTTTAGGGGAAATTATCTACAGTTTTTTTGATAAGTTAAAGTCGATTAGTAAGGGTTATGCATCGTTAAACTATGAGTTTTATAACTACCAACATAGTCAACTGGAAAAAGTTGAGATCATGTTAAACAAACAAAAGATTGATGCATTATCTTTTATCAGTCATAAAGACTTTGCTTTTAAGCGGGCAAAAAAGTTTTGCACTAAGCTCAAAGAATTGATTCCAAAGCATCTGTTTGAGATCCCTATCCAAGCAACAATAGGGAGTAAAGTAATAGCAAGAGAGACAATCAAAGCAGTTAGAAAGGATGTAATAGCTAAACTTTATGGAGGGGATGTTAGTAGAAAAAAGAAGTTATTAGAGAAGCAAAAAGAGGGTAAAAAACGCTTGAAAGCAGTTGGGAGTGTTCAATTACCTCAAGAGCTATTTAGTCATTTGCTGAAAGATGAAGATTAA", "aa": "MEQKNIRNFSIIAHIDHGKSTLSDRLLEHSLGFEKRLLQAQMLDTMEIERERGITIKLNAVELKINVDNNNYLFHLIDTPGHVDFTYEVSRSLAACEGVLLLVDATQGIQAQTISNAYLALENNLEIIPVINKIDMDNADIETTKDSLHNLLGVEKNSICLVSAKANLGIDQLIQTIIAKIPPPKGEINKPLKALLFDSYYDPYKGVVCFIRVFDGCLKVNDKVRFIKSNSVYQIVELGVKTPFFEKRDQLQAGDVGWFSAGIKKLRDVGVGDTIVSFDDQFTKPLAGYKKILPMIYCGLYPVDNSDYQNLKLAMKKIIISDAALEYEYETSQALGFGVRCGFLGLLHMDVIKERLEREYNLKLISAPPSVVYKVLLTNGKEISIDNPSLLPERSKIKAISEPFVKVFIDSPDQYLGSVIDLCQNFRGQYESLNEIDINRKRICYLMPLGEIIYSFFDKLKSISKGYASLNYEFYNYQHSQLEKVEIMLNKQKIDALSFISHKDFAFKRAKKFCTKLKELIPKHLFEIPIQATIGSKVIARETIKAVRKDVIAKLYGGDVSRKKKLLEKQKEGKKRLKAVGSVQLPQELFSHLLKDED", "aa_hexdigest": "2eb228b3a5638bc2136feaf8bed63fe8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3987", "ncbi_nrp_id": "WP_014894168.1", "uniref100_id": "UniRef100_UPI00027B3987", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3987", "RefSeq:WP_014894168.1", "UniRef:UniRef100_UPI00027B3987" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3987", "uniref90_id": "UniRef90_P47384" }, "psc": { "uniref90_id": "UniRef90_P47384", "gene": "lepA", "product": "translation elongation factor 4", "ec_ids": [ "3.6.5.n1" ], "uniref50_id": "UniRef50_B1X3K4", "cog_id": "COG0481", "cog_category": "J", "go_ids": [ "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005886", "GO:0043022", "GO:0045727", "GO:1990606" ] }, "genes": [ "lepA" ], "id": "KMADJIBOEG_345", "locus": "GCA000292445_00306" }, { "type": "cds", "sequence": "contig_1", "start": 165438, "stop": 167096, "strand": "+", "frame": 3, "gene": "rnj", "product": "Ribonuclease J", "db_xrefs": [ "COG:COG0595", "COG:J", "EC:3.1.-.-", "GO:0003723", "GO:0004521", "GO:0004534", "GO:0005737", "GO:0006364", "GO:0008270", "SO:0001217", "UniRef:UniRef50_P75497", "UniRef:UniRef90_P47385" ], "nt": "ATGATAAAGGATTTTAATCCTGGTGATTTTATTGGTAAAAAACCAACTAAAATCTATGCTTTTGGTGGTATCCAAGAAGTTGGTAAAAACATGTATGGGATTGAATATGATGATGAAATCATCATTATTGACTGTGGCATTAAATTTGCTAGTGATGATCTACTTGGTATCAATGGGATTATCCCTAGTTTTGAACACTTAATTGAAAACCAAAGTAAGGTTAAAGCATTGTTTATTACCCATGGTCATGAAGACCATATTGGGGGTGTACCATACCTTTTAAAGCAGGTTGATATTCCTGTTATCTACGCACCAAGGATCGCAGCATCATTAATCTTGAAAAAGGTTAATGAGCACAAGGATGCTAAGCTCAATAAGATAGTTACTTTTGATGATTTTAGTGAGTTTCAAACCAAACACTTCAAAATTGATTTTTACCGGGTAAACCACTCGATTCCCGATGCTTTTGGAATCTGTGTGCAAACCCCTAATGGCAACATTGTTCAAAGTGGTGACTACCGGTTTGATTTTGCTGCTGGGAGTGAGATGTTAGATGTTCATAAAGTAGTGAAAATTGCTGAGCGTAATGTCCATGTTTTTATGAGTGAATCTACTAATGCTGAAGTACCAGGTTTTTCCCAAAGTGAAAAGTTAATTTACAGAAACATCCAAAAGATCTTAAAAGAAGCAAGAGGTAGGGTTATTTTAACTACCTTTGCATCCAACATCACACGGATTAATGAAATTATTGAGATTGCTTTAAACAACAAGCGCAAGATCTGTTTATTGGGCAAATCAATGGATGTTAATGTCAATATTTCGCGCAAAATTGGATTGATGGCAATTGATAGTAATGATATTGTGGAAGTCCGTGATATCAAAAACTATCCTGATCGTAATATCTTAATCTTGTGTACTGGTTCACAAGGCGAGGAGGCTGCTGCTTTAAACACGATGGCACGTGGTAAGCATAATTGGGTAAGCTTAAAATCAACTGACACCATCATTATGTCTTCAAATCCAATTCCAGGTAATTATGCTGCAGTTGAAAACTTGCTTAATGAACTATCTAAGTTTGGTGTTGCTATTTATGAAAATTCATCCCAACTAAAACTACATGCCTCAGGTCATGCCACCCAACAAGAGTTACAGTTGATGCTAAATTTAATGTTTCCTAAATACTTAATTCCTATCCATGGTGAATTTAAGATGATGCGAACCATAAAAAACATTGCTAATGAATGTGGTATTAAAAGCGAGGATGTGGCACTTTTAAGTAATGGCCAAGTAATGTATTTAATTGATGAAGAACTTTATTATTCCAATGAAATTATTAATGCTGATCCTATTTATATAGAGAGTCATAACTCTTCTCCTGATCTTGCAAGAATAATTAAGCAAAGACAAATCCTTAGTCGTGATGGGATGTTTGCTGTTATTGTTGTTTTTGATAAAAATAACAACATCATTGGGATTCCAACCTTAATAACAAGAGGTTGTTTCTTTGCACTTGATTCCAATCCTTTAATGACAAAGATAGCCCATTCTGTTAAAAGAACTTTAGAAAGTGTTATCCAAAGTAAGAAGTTTAATAGTCATGAACAACTAACAAAGGAATTGAAACGAGTTTGTAAGGAAACTGTTTCTTACTTTATCTGA", "aa": "MIKDFNPGDFIGKKPTKIYAFGGIQEVGKNMYGIEYDDEIIIIDCGIKFASDDLLGINGIIPSFEHLIENQSKVKALFITHGHEDHIGGVPYLLKQVDIPVIYAPRIAASLILKKVNEHKDAKLNKIVTFDDFSEFQTKHFKIDFYRVNHSIPDAFGICVQTPNGNIVQSGDYRFDFAAGSEMLDVHKVVKIAERNVHVFMSESTNAEVPGFSQSEKLIYRNIQKILKEARGRVILTTFASNITRINEIIEIALNNKRKICLLGKSMDVNVNISRKIGLMAIDSNDIVEVRDIKNYPDRNILILCTGSQGEEAAALNTMARGKHNWVSLKSTDTIIMSSNPIPGNYAAVENLLNELSKFGVAIYENSSQLKLHASGHATQQELQLMLNLMFPKYLIPIHGEFKMMRTIKNIANECGIKSEDVALLSNGQVMYLIDEELYYSNEIINADPIYIESHNSSPDLARIIKQRQILSRDGMFAVIVVFDKNNNIIGIPTLITRGCFFALDSNPLMTKIAHSVKRTLESVIQSKKFNSHEQLTKELKRVCKETVSYFI", "aa_hexdigest": "ce85e26158e04109b5549b8cf0c48792", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47385", "query_cov": 1.0, "subject_cov": 0.9701230228471002, "identity": 1.0, "score": 1091.0, "evalue": 0.0, "valid": true, "gene": "rnj", "product": "Ribonuclease J", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75497", "cog_id": "COG0595", "cog_category": "J", "go_ids": [ "GO:0005737", "GO:0004534", "GO:0003723", "GO:0004521", "GO:0008270", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_P75497", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75497" ], "product": "Ribonuclease J" }, "genes": [ "rnj" ], "id": "KMADJIBOEG_346", "locus": "GCA000292445_00307" }, { "type": "cds", "sequence": "contig_1", "start": 167166, "stop": 167519, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTACTAGTGCCAAAAGTGGTATTGGAGTATCACAACCTGAATAACCAAGTAGTCAAAGAGAGTTTGGAAGTGGAAGCAACTGATTCTTTCAACCCCACCCAAAGGTTGCAAAAAGAGAGTCCAGAAAAGGATGATAGTAAAAGTCAGGAGAAACTCCAAGAGACCATGTCATCAGTGAGTATGAGTGGGGCTACCTCTACTCGAACCAAGGCCCTCAAAGTGGAGGTGGAAAGGGGTAGTAAAGTCAACCAAGGCGAACTACAATCCAACGACTTTGCCAAAAAGCCGTTTAAAGATGAGAGCAATAAGAAGTTGGATGCGAGTGGGGAGTTTGCCAATGATAAAGCCTGA", "aa": "MLLVPKVVLEYHNLNNQVVKESLEVEATDSFNPTQRLQKESPEKDDSKSQEKLQETMSSVSMSGATSTRTKALKVEVERGSKVNQGELQSNDFAKKPFKDESNKKLDASGEFANDKA", "aa_hexdigest": "42cd5df56a29fc38c5505bc14ed017fc", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FCE6354", "query_cov": 0.9743589743589743, "subject_cov": 0.8636363636363636, "identity": 0.693, "score": 125.0, "evalue": 5.3e-35, "valid": false, "product": "adhesin", "uniref50_id": "UniRef50_A0A7M3VDG5" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13048.374499999984, "isoelectric_point": 5.510619926452636 }, "id": "KMADJIBOEG_347", "locus": "GCA000292445_00308" }, { "type": "cds", "sequence": "contig_1", "start": 167944, "stop": 168189, "strand": "+", "frame": 1, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002FF8E11", "UniRef:UniRef50_UPI001ED95422", "UniRef:UniRef90_UPI0002FF8E11", "UniRef:UniRef90_UPI001ED95422" ], "nt": "TTGACTTTCACTAATAAGAATAACCCGCAACGAAATCAGTTGTTGCTCAGAAGCTTACTAGGAACGATCCCGGTATTGATCAATAAGAGTGGGGATAGTAATGATCAATTTAACAAGGATAGTGACCAGAAATGGGATAAAACGAATGAAAAAGATGGGAATTTACCTGGGTTTGGGGAGGTGAATGGTGGTTTTTACCCAGTTTTTACTTATTTAATAGTTAAAAAAGTTTTAGATTTTTCTTAG", "aa": "MTFTNKNNPQRNQLLLRSLLGTIPVLINKSGDSNDQFNKDSDQKWDKTNEKDGNLPGFGEVNGGFYPVFTYLIVKKVLDFS", "aa_hexdigest": "4164f682cb84acbc1ad03de1901076a1", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001ED95422", "query_cov": 0.8395061728395061, "subject_cov": 1.0, "identity": 0.912, "score": 134.0, "evalue": 1.21e-39, "valid": true, "uniref50_id": "UniRef50_UPI001ED95422" }, "pscc": { "uniref50_id": "UniRef50_UPI001ED95422", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED95422" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 167693, "stop": 168189, "observations": { "deletion": [ 167864 ], "mutation": [ 167762 ], "stop": [ 167762 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0002FF8E11", "query_cov": 1.0, "subject_cov": 0.4879518072289157, "identity": 0.9259999999999999, "score": 160.0, "evalue": 5.06e-49, "gene_start": 1, "gene_end": 81, "reference_start": 86, "reference_end": 166, "reference_sequence": "MITQHLNKENTRWVFIPNFSPDIWTGAGYRKANNNNNGIPFEQVKPSNSSTPFNPTSAGGSSAKKTTTYSFLPNSISPTSDWINALTFTNKNNPQRNQLLLRALLGTIPVLINKSGDSNDQFNKDSEQKWDKTNEKDGNLPGFGEVNGGFYKIFTYLIVKKVLNFS" }, "paralog": false, "identity": 0.9096385542168675, "score": 306.0, "evalue": 9.32e-109, "description": "Internal stop codon at 167762. Frameshift due to deletion around 167864. Nonsense mutation around 167762.", "psc": { "uniref90_id": "UniRef90_UPI0002FF8E11", "product": "adhesin", "uniref50_id": "UniRef50_UPI0002FF8E11", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0002FF8E11", "UniRef:UniRef50_UPI0002FF8E11" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0002FF8E11", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002FF8E11" ], "product": "adhesin" } }, "id": "KMADJIBOEG_348", "locus": "GCA000292445_00309" }, { "type": "cds", "sequence": "contig_1", "start": 168298, "stop": 168477, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGGTATTTGAGCCACTTGAACTCATACTTAACTTTCACCTGAGTAGACTTGAACAACACAAACAAGTTGGTGTTATGGATGGTTTGGTCGGAGATTTGATAGGAAGAATCAAGTTGTGCCAAACTAACCAGATCACTTTTAGAATCGGTGATAGGGGTGGCGAAGTAAAAACCTAA", "aa": "MLVFEPLELILNFHLSRLEQHKQVGVMDGLVGDLIGRIKLCQTNQITFRIGDRGGEVKT", "aa_hexdigest": "ccc9ed4e5061dceec8f9a45e6fe28617", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6677.774799999999, "isoelectric_point": 6.707819557189941 }, "id": "KMADJIBOEG_349", "locus": "GCA000292445_00310" }, { "type": "cds", "sequence": "contig_1", "start": 168566, "stop": 168742, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13", "UniRef:UniRef90_UPI0030D43D23" ], "nt": "ATGGTGAAAAAATATCAGTTAGATCTACCAAGTGTTTCAACAACCATGAACCAAGTGTTGCAAGAATTGATCCTAGAACAACCTTTGACTAAGTATACCTTAAATTCGAGTTTGGCCAAACAAAAGGGCAAAAGCCAGATAGAGGTGCATCTGGGTAGTGGGCAAGCAAATCAGTGA", "aa": "MVKKYQLDLPSVSTTMNQVLQELILEQPLTKYTLNSSLAKQKGKSQIEVHLGSGQANQ", "aa_hexdigest": "c4eb3decf832ce49478c8fa4edca0275", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 168440, "stop": 168808, "observations": { "mutation": [ 168560, 168740 ], "stop": [ 168560, 168740 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0030D43D23", "query_cov": 0.9827586206896551, "subject_cov": 0.47107438016528924, "identity": 0.877, "score": 97.8, "evalue": 4.47e-25, "gene_start": 2, "gene_end": 58, "reference_start": 43, "reference_end": 99, "reference_sequence": "MKLTYSSSGSNTNTISFENNANKPSYVVEFTNSTNVGIKWRVVKKYQLDVPNVSSDMNQVLQELILEQPLTKYTLNSSLAKQKGKSKIEVHLGSGRATQWTSRTQLHWPKQQSQPKCINRI" }, "paralog": false, "identity": 0.7540983606557377, "score": 173.0, "evalue": 2.12e-57, "description": "Internal stop codon at 168560, 168740. Nonsense mutation around 168560, 168740.", "psc": { "uniref90_id": "UniRef90_UPI0030D43D23", "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0030D43D23", "UniRef:UniRef50_UPI00027B3D13" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6458.374700000002, "isoelectric_point": 9.158652687072756 }, "id": "KMADJIBOEG_350", "locus": "GCA000292445_00311" }, { "type": "cds", "sequence": "contig_1", "start": 169264, "stop": 169488, "strand": "+", "frame": 1, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030D266AC", "UniRef:UniRef90_UPI00027B3D0D" ], "nt": "GTGACCAACGACACGGGATTAAATGGTCCAATCAATGGAAGTTTTTCAATCCAAGACACCTTCAGCTTTGTTGTTCCTTATTCGGGGAATCATAGTAATCAAACTTCATCAGGACCCATTAAAACTGCTTATCCGGTGAAAAACACAGAAAAATCAACTGTCAAGATCAATTCCTTGATCAATGCTACGCCGTTGAATAGTTATGGGGATTTAAACATTAAATAA", "aa": "MTNDTGLNGPINGSFSIQDTFSFVVPYSGNHSNQTSSGPIKTAYPVKNTEKSTVKINSLINATPLNSYGDLNIK", "aa_hexdigest": "64068847154f70caaf3733999a38b969", "start_type": "GTG", "rbs_motif": null, "genes": [ "mgpC" ], "truncated": "3-prime", "pseudogene": { "start": 169294, "stop": 169523, "observations": { "deletion": [ 169480 ], "stop": [ 169483 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00027B3D0D", "query_cov": 0.8108108108108109, "subject_cov": 0.6060606060606061, "identity": 0.9, "score": 105.0, "evalue": 2.98e-28, "gene_start": 13, "gene_end": 72, "reference_start": 26, "reference_end": 85, "reference_sequence": "MTSLLKLLSSKNIKQKLGKGGTAMQGSFSVQDTFSFVVPYSGNHSNQISSGTIKTAYPVKKDQKSTVKINSLINATPLNSYGDLNNHTKLIYPNYLLIY" }, "paralog": false, "identity": 0.8289473684210527, "score": 124.0, "evalue": 3.16e-38, "description": "Internal stop codon at 169483. Frameshift due to deletion around 169480.", "psc": { "uniref90_id": "UniRef90_UPI00027B3D0D", "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI0030D266AC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00027B3D0D", "UniRef:UniRef50_UPI0030D266AC" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0030D266AC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030D266AC" ], "product": "MgpC family cytadherence protein" } }, "id": "KMADJIBOEG_351", "locus": "GCA000292445_00312" }, { "type": "rRNA", "sequence": "contig_1", "start": 169685, "stop": 171202, "strand": "+", "gene": "rrs", "product": "16S ribosomal RNA", "coverage": 0.9902152641878669, "score": 1442.0, "evalue": 2e-44, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00177", "KEGG:K01977", "SO:0001000" ], "nt": "TTTTCTGAGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCATGCCTAATACATGCAAGTCGATCGGAAGTAGCAATACTTTAGAGGCGAACGGGTGAGTAACACGTATCCAATCTACCTTATAATGGGGGATAACTAGTTGAAAAACTAGCTAATACCGCATAAGAACTTTAGTTCGCATGAATTAAAGTTGAAAGGACCTGCAAGGGTTCGTTATTTGATGAGGGTGCGCCATATCAGCTAGTTGGTAGGGTAATGGCCTACCAAGGCAATGACGTGTAGCTATGCTGAGAAGTAGAATAGCCACAATGGGACTGAGACACGGCCCATACTCCTACGGGAGGCAGCAGTAGGGAATTTTTCACAATGAGCGAAAGCTTGATGGAGCAATGCCGCGTGAACGATGAAGGTCTTTTTGATTGTAAAGTTCTTTTATTTGGGAAGAATGACTCTAGCAGGCAATGGCTGGAGTTTGACTGTACCACTTTGAATAAGTGACGACTAACTATGTGCCAGCAGTCGCGGTAATACATAGGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCAAGCGCAGGCGGATTGAAAAGTCTGGTGTTAAAGGCAGCTGCTTAACAGTTGTATGCATTGGAAACTATCAGTCTAGAGTGTGGTAGGGAGTTTTGGAATTTCATGTGGAGCGGTGAAATGCGTAGATATATGAAGGAACACCAGTGGCGAAGGCGAAAACTTAGGCCATTACTGACGCTTAGGCTTGAAAGTGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACACCGTAAACGATAGATACTAGCTGTCGGAGCGATCCCTTCGGTAGTGAAGTTAACACATTAAGTATCTCGCCTGGGTAGTACATTCGCAAGAATGAAACTCAAACGGAATTGACGGGGACCCGCACAAGTGGTGGAGCATGTTGCTTAATTCGACGGTACACGAAAAACCTTACCTAGACTTGACATCCTTGGCAAAGTTATGGAAACATAATGGAGGTTAACCGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCGTTAGTTACATTGTTTAACGAGACTGCTAATGTAAATTGGAGGAAGGAAGGGATGACGTCAAATCATCATGCCCCTTATGTCTAGGGCTGCAAACGTGCTACAATGGCCAATACAAACAGTAGCCAACTTGTAAAAGTGAGCAAATCTGAAAAGTTGGTCTCAGTTCGGATTGAGGGCTGCAATTCGTCCTCATGAAGCTGGAATCACTAGTAATCGCGAATCAGCTATGTCGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACTATGAAAGCTGGTAATATTTAAAAACGTGTTGCTAACCTTTATTGGAAGCGCATGTCAAGGATGGCACCGGTGATTGGAGTTAAGTCGTAACAAGGTACCCCTACGAGAACGTGGGGGTGGATCACCTCCTTT", "id": "KMADJIBOEG_38", "locus": "GCA000292445_00313" }, { "type": "rRNA", "sequence": "contig_1", "start": 171402, "stop": 174317, "strand": "+", "gene": "rrl", "product": "23S ribosomal RNA", "coverage": 0.9969230769230769, "score": 2681.4, "evalue": 6.1e-06, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF02541", "KEGG:K01980", "SO:0001001" ], "nt": "CAATAAGTTACTAAGGGCTTATGGTGGATGCCTTGGCACTAAAAGGCGATGAAGGACGTGTTAACCTGCGATAAGCTTCGGGGAGGTGGTAAAAACCTGAGATCCGGAGGTGTCCGAATGGAGCAATCTGGTAGCTTGCAAAAGTTACCATTAATTAATGAATTCATAGTTAATTAAAGCGATACGTGGTGAAGTGAAACATCTCAGTAACCACAGGAAAAGAAAACGAATGTGATTCCGTGTGTAGTGGCGAGCGAAAGCGGAACAGGCCAAACCTATCTGAGGATAGGGGTTGTAGGGCTTGCATTATGGAAGTTAAAAGGATAGAAGAAGCTGTTGGAAAGCAGCGCCAAAGAGGGTGATAGCCCCGTATTTGAAATCTTTTTAATACCTAGCAAGAAACCTGAGTAGCTCGAAAAACGTTATTTTGAGTGAATCTGCCCAGACCATTGGGTAAGCCTAAATACTAATTAGTGACCGATAGCGAAACAGTACCGTGAGGGAAAGGTGAAAAGAACCCAGAGATGGGAGTGAAATAGATTCTGAAACCATATGCCTACAACGTGTCAGAGCACATTAATGTGTGATGGCGTGCGTTTTGAAGTATGAGCCGGCGAGTTATGATAGCAAGCGTTAGTTAACCAGGAGATGGGGAGCTGTAGCGAAAGCGAGTTTTAAGAGAGCGTTTGTTTGTTATCATAGACCCGAAACGGGTTGAGCTAGTCATGAGCAGGTTGAAGGTTGAGTAACATTAACTGGAGGACCGAACCGACTCTCGTTGAAACGATAGCGGATGACTTGTGATTAGGGGTGAAATTCCAATCGAAATCCGTGATAGCTGGTTCTCGTCGAAATAGCTTTAAGGCTAGCGTAAGATCACAAATAAGTGGAGGTAAAGCTACTGAATGTATGATGGCGCCACCTAGGCGTACTGAATACAATTAAACTCTGAATGCCATTTATTTTATTCTTGCAGTCAGACAGTGGGGGATAAGCTTCATTGTCAAGAGGGGAAGAGCCCAGATCATTAAATAAGGTCCCCAAAATATACTAAGTGGAAAAGGATGTGAAAGTGCTAAAACAGCAAGGATGTTGGCTTAGAAGCAGCCATCGTTTAAAGAGTGCGTAACAGCTCACTTGTCGAGTGTTTTTGCGCCGAAGATGTAACGGGGCTAAGTATATTACCGAATTTATGGATAAGATATTTTTATCTTGTGGTAGACGAGCGTTGTATTGGAGTTGAAGTCAAAGCGTGAGCATTGGTGGATCCAATACAAGTGAGAATGCCGGCGTGAGTAACGCTTGGGAGTGAGAATCTCCCAAACCGATTGACTAAGGTTTCCTGGACCAGGGTCGTCCTTCCAGGGTTAGTCTGGACCTAAGCTGAGGCTGAAGAGCGTAGGCGATGGACAACAGGTTAATATTCCTGTACTTACAGTTAGACTGATGGAGTGACAAAGAAGGTTATCCACCCCCATTATTGGATTTGGGGATAAACCATAAGGTGGTACAATAGGCAAATCCGTTGTGCATAACACTGAGTGGTGATGTCGAGTGAACGAGTGATCAAGTAGCGAAGGTGGCAATTAATCATGCTTTCAAGAAAAGCTGCTAGGGCTAATTTAACTGTAACCAGTACCGAGAACGAACACACGTAGTCAAGGAGAGGATCCTAAGGTTAGCGAGTGAACTATAGCCAAGGAACTCTGCAAATTAACCCCGTAAGTTAGCGAGAAGGGGTGCTTATCTAAAAGTAAGCCGCAGTGAAGAACGAGGGGGGACTGTTTAACTAAAACACAACTCTATGCCAAACCGTAAGGTGATGTATATGGGGTGACACCTGCCCAGTGCTGGAAGGTTAAAGAAGGAGGTTAGCAATTTATTGCAAAGCTTTTAACTGAAGCCCCAGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTAGTCGGGTAAATTCCGTCCCGCTTGAATGGTGTAACCATCTCTTGACTGTCTCGGCTATAGACTCGGTGAAATCCAGGTACGGGTGAAGACACCCGTTAGGCGCAACGGGACGGAAAGACCCCGTGAAGCTTTACTGTAGCTTAATATTGATCAAAACACCACCATGTAGAGAATAGGTAGGAGCAATTGATGCAAGTTCGCAAGGATTTGTTGATGTGAAATGTGGAATACTACCCTTGGTTATGTTTTGTTCTAATTGGTAACTGTGATCCAGTTTCAAGACAGTGTTAGGTGGGCAGTTTGACTGGGGCGGTCGCCTCCTAAAAGGTAACGGAGGCGCACAAAGGTACCTTCAGTACGGTTGGAAATCGTATTTAGAGTGTAATGGTATAAGGGTGCTTGACTGTGAGACTTACAGGTCGAACAGGTGAGAAATCAGGTCATAGTGATCCGGTGGTTCAGTATGGAATGGCCATCGCTCAACGGATAAAAGCTACTCCGGGGATAACAGGCTGATACTGCCCAAGAGTTCATATCGACGGCAGTGTTTGGCACCTCGATGTCGACTCATCTCATCCTCGAGCTGAAGCAGGTTCGAAGGGTTCGGCTGTTCGCCGATTAAAGAGATACGTGAGTTGGGTTCAAACCGTCGTGAGACAGGTTGGTCCCTATCTATTGTGCCCACAGGAAGATTGAAGAGCTTTGCTTCTAGTACGAGAGGACCGGAGCGAGGACACCGCTTATGCTCCAGTTGTAGCGCCAGCTGCACCGCTGGGTAGTAACGTGTCTATTAGATAAACGCTGAAAGCATCTAAGTGTGAAACTATCTCAAAGATTAATCTTCCCATTTCTGTTAAAGGAAAGTAAGAGCCGTTATAGACCATGACGTTGATAGGTTACAGGTGTAAGCATAGTGATATGTTGAGCTGAGTAATACTAATTGCTCGAGGACTTATTGGT", "id": "KMADJIBOEG_40", "locus": "GCA000292445_00314" }, { "type": "rRNA", "sequence": "contig_1", "start": 174358, "stop": 174466, "strand": "+", "gene": "rrf", "product": "5S ribosomal RNA", "coverage": 0.9159663865546218, "score": 72.8, "evalue": 5.1e-12, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00001", "KEGG:K01985", "SO:0000652" ], "nt": "TTTGGTGCTAATATCGCTGTGGAAACACCTGGAACCATCCCGAACCCAGCAGTTAAGCACAGTGGAGCTAAATGTAGGTAGTAATACTGAGAATAGGTAAGCACCAAGC", "id": "KMADJIBOEG_39", "locus": "GCA000292445_00315" }, { "type": "cds", "sequence": "contig_1", "start": 175633, "stop": 175923, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACCAACAGGATAAACTTGAATTTAACTTAACAAGATTAAAGGAGATAGATGTTGAAGATGGTAAGCAACTATTTGGGATAGAAACTAGTGTTTATAAACACTTTCAAAATGAAATTGCTCGTTTTTATAAACAAGTAAACAAACACTTTCGTGAAACAGGTAGTGAAAGTTTGTTTTTAGCTTTACCAGTTATTGAAGGGATTAATGAGTTTAACGATATCTTTCGAGCTCCATTACTTTATGTTGGAGTTAAACTCAAAGTTTCCCCACGCTTTGAACGTTTCTGA", "aa": "MNQQDKLEFNLTRLKEIDVEDGKQLFGIETSVYKHFQNEIARFYKQVNKHFRETGSESLFLALPVIEGINEFNDIFRAPLLYVGVKLKVSPRFERF", "aa_hexdigest": "f2df0dde154fc7de8d26cbbee3a24d31", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11369.886599999989, "isoelectric_point": 6.736182212829591 }, "id": "KMADJIBOEG_352", "locus": "GCA000292445_00316" }, { "type": "cds", "sequence": "contig_1", "start": 175996, "stop": 176847, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12" ], "nt": "TTGTTTAAAAATAACTATGATACTACTAAGATAGATGTTAATGATGCTTTAAAGGTATTTAGTGAACTTGAATATGAGTTTAGAATGCCTTTAACTTCTGAATTGAAGAGTTTTAGTAAAAAAGCAAAGAGTGATTTTAATACTGAAAAACGAACTAACTATCTTATTAACAACGTTCTTTTGGGGATCTTTGATGTTAAGGGTGATCAGCTGTTCCAAAACTTTAATGAGATTCTAAACACTGATCCTGATGTATTAGATGAACTTCTAAAAGATAGAAGAGATCTGTTGTTAGAAAACCGGGAATTCCGTGAACAATTTGATTTAAAAGATACCTATCTCTTCAGTCACCTTGATATCTACCAACAGTATGCAGTTAAGCAAGCTTTACTTGGTGATTTAATTATTGAAGGCCCACCTGGCACAGGGAAATCTGAAACAATTGTTAATATCTTAGTTAACCTTGTTTTAAACAACAAAAAAGTATTGTTTGTTTCTGAAAAAGTAACTGCACTTGATGTTGTTTACAACCGTCTTGGTAGTTTTAAACACATCGCACTTTTCAACGCTAGTGTTGCTGCTGAAAAGAAACGCTTTTATAACCAGTTTGCTGAGTTTGAAACTTATTTCACTACTTACTTTTCCAAGAAAGATTTGGATGCTACTTTACCAACATTTGAAGGTAAATGGGTGGATGATATTTTAGAGGCATTTCAAGCATTACAAGCTCTTTATGACACCAAGATAAATTCTGGTGAAAATCTGTTTAGTTTCAAAGAGATTGTCAGTAGCTTTCAGATGTTGGATGCTAGTTACATCAAGATTAAAGAATATGAACGTTTTGATGAGTGA", "aa": "MFKNNYDTTKIDVNDALKVFSELEYEFRMPLTSELKSFSKKAKSDFNTEKRTNYLINNVLLGIFDVKGDQLFQNFNEILNTDPDVLDELLKDRRDLLLENREFREQFDLKDTYLFSHLDIYQQYAVKQALLGDLIIEGPPGTGKSETIVNILVNLVLNNKKVLFVSEKVTALDVVYNRLGSFKHIALFNASVAAEKKRFYNQFAEFETYFTTYFSKKDLDATLPTFEGKWVDDILEAFQALQALYDTKINSGENLFSFKEIVSSFQMLDASYIKIKEYERFDE", "aa_hexdigest": "ecc853eb3e071fcaa92a9369f21bc93b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 73, "aa_cov": 0.22614840989399293, "hmm_cov": 1.0, "evalue": 1.2739774740432186e-09, "score": 33.35270690917969, "start": 118, "stop": 181 } ], "seq_stats": { "molecular_weight": 33010.02810000003, "isoelectric_point": 4.907330894470214 }, "id": "KMADJIBOEG_353", "locus": "GCA000292445_00317" }, { "type": "cds", "sequence": "contig_1", "start": 176902, "stop": 177222, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAAAGAACTTAGTCAGCGCTGGAATGGTATTGATAATTTCTATCAGTTGCAATCGCTTCTAAACCAAACCAAAAAACGAAAGGTCTTAAACTATGTGTTGGAACACTTTGAACAGTTTAATACAGTTATCAGTCCTAAGCATGTTTTGTTCTACAAGCCTAGCAATAAATCACAATTGCTCTTAAAACAACTGAAACAGGATGTTGAACAATACACTAGTTTACAACGTTTCCAATCTCCTACTAAGTTTGAAACAATCAAGTTGAATTTCATCAACCAAGTTAATGAAAACCCAACCCCATGGTTCTTTTCTTGA", "aa": "MKKELSQRWNGIDNFYQLQSLLNQTKKRKVLNYVLEHFEQFNTVISPKHVLFYKPSNKSQLLLKQLKQDVEQYTSLQRFQSPTKFETIKLNFINQVNENPTPWFFS", "aa_hexdigest": "60f7feb5b772c56f979b4cfafbb6c1d6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12827.560899999988, "isoelectric_point": 9.776324272155762 }, "id": "KMADJIBOEG_354", "locus": "GCA000292445_00318" }, { "type": "cds", "sequence": "contig_1", "start": 177250, "stop": 177942, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12" ], "nt": "TTGGAAAAACTCGTTAGTTTTGAGTCAAACATTATTAAAACAAAACAAGCTTATCTTAATGGGATTGAAAGCTATGTAGCAAGTTGTAAGAAACTGCTTAAAACAACTATTTTAAACAACTTTTTTCAGCTTTATCAAACTAATAAAGATGAACTATTGGAGATCTGCAGACAAGCAAAAAACCCAGTTTTAAAAGAGATTACTTGGTGGTTTAAAAAACATTTTGAACTCTTAAAAAAACTCTTTCCAGTGCACATTATGACCCTTGAGTCTGCAGCAACTCTAACCCCTAACCAACGTGGTTTGTATGACTATGTGGTTATTGATGAAGCTAGTCAAGTATTTCTAGAAAGAGCAATTCCTATCTTATTTAGAGCTGATAAGTACATTATTGCAGGGGACACTAAACAGTTAAAACCAGCTAACTTCTTCCAATCACGTGCTGAGTATGATGTTGATGAGGAGTTTGAAGATGGCAATATAGAAGCTGCTGTTCACTCCAGTTCTCTACTTCATTTTTTAAAGAACCGTTCAAGAATCTTAACTTTACTTAAGTTCCACTACCGCAGTGATTCAGCTGATCTGATTGCTTTTACTAATAACAGGATCTATGACAATGAATTAATCTTTATGAATAAAGCTAATGCTGATCAAAGGGTTTTTATTGTCCATGATGTAATAGATGGTATCTGA", "aa": "MEKLVSFESNIIKTKQAYLNGIESYVASCKKLLKTTILNNFFQLYQTNKDELLEICRQAKNPVLKEITWWFKKHFELLKKLFPVHIMTLESAATLTPNQRGLYDYVVIDEASQVFLERAIPILFRADKYIIAGDTKQLKPANFFQSRAEYDVDEEFEDGNIEAAVHSSSLLHFLKNRSRILTLLKFHYRSDSADLIAFTNNRIYDNELIFMNKANADQRVFIVHDVIDGI", "aa_hexdigest": "e6aee446c4281807e0d2828a137a8f41", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 54, "aa_cov": 0.23043478260869565, "hmm_cov": 1.0, "evalue": 3.152012265510958e-10, "score": 35.340450286865234, "start": 88, "stop": 140 } ], "seq_stats": { "molecular_weight": 26793.49060000002, "isoelectric_point": 6.603235816955567 }, "id": "KMADJIBOEG_355", "locus": "GCA000292445_00319" }, { "type": "cds", "sequence": "contig_1", "start": 177979, "stop": 178113, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAACGCTTAGAACAACTAACAACAACCAATGATTACAAAAAGTCACTTGGTGTAATTTGTTTCAATAAAAACCAAGCAGATCTAATTGAATACTTAATTGATAAACAAAACAATCCGCTACTTAACGAGTGA", "aa": "MQRLEQLTTTNDYKKSLGVICFNKNQADLIEYLIDKQNNPLLNE", "aa_hexdigest": "3284f53694d67ee22a658d358df2cd28", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5170.8263, "isoelectric_point": 5.145031547546386 }, "id": "KMADJIBOEG_356", "locus": "GCA000292445_00320" }, { "type": "cds", "sequence": "contig_1", "start": 178156, "stop": 178734, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13087.12" ], "nt": "GTGAAAAACATTGAAAATGTGCAGGGTGATGAAAGGGATATCATCATCTTCTCTTTAGGTTATGATCGTTCAGTAAATAGTTATGGTCCTATTAGTAAGCAAGGTGGGGAAAACAGACTAAATGTAGCGATAACTAGAGCTAAACAAAGAATAGAACTGTTTAAGACAAATAGAGGGGAAGACTACAATGGTTTAAACTCCAGTTCTTTGGGTAGTAAACTGTTAGTTGAATATCTACTTTATTGTGAAGCAATGGCTAAAAATCAGGGTGAGAAAATCACTTTTCAAGCGGTAAAAAGAAAAGAAACAAAAGCAAAGTATGAACTAGCAGTTGAAAATGATTTCTTCAACCAACTGCAGGCAATTTTTGGCGGAGAGTTTGAGATTAAACGTAACGTTAATGAAGGAGCTTACTTTTTCTCATTTGTCTTTTACTTTAATAATATCCCTTATCTTGCCATTGACTTTAATATCCCCATTCCCACTTCAAGAAAACAAGTTATGGAAGGGATTTTATACCGTGAACAGTTTCTCAAAAAACGTCAATGGAACCTAATTAACATCTGGATTGATGAGTGA", "aa": "MKNIENVQGDERDIIIFSLGYDRSVNSYGPISKQGGENRLNVAITRAKQRIELFKTNRGEDYNGLNSSSLGSKLLVEYLLYCEAMAKNQGEKITFQAVKRKETKAKYELAVENDFFNQLQAIFGGEFEIKRNVNEGAYFFSFVFYFNNIPYLAIDFNIPIPTSRKQVMEGILYREQFLKKRQWNLINIWIDE", "aa_hexdigest": "90ddedb3aa85d1cc628f5344f222fa17", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13087.12", "name": "AAA_12", "length": 50, "aa_cov": 0.2604166666666667, "hmm_cov": 0.94, "evalue": 5.0343231513280106e-09, "score": 31.116132736206055, "start": 2, "stop": 51 } ], "seq_stats": { "molecular_weight": 22399.22600000001, "isoelectric_point": 8.510100746154787 }, "id": "KMADJIBOEG_357", "locus": "GCA000292445_00321" }, { "type": "cds", "sequence": "contig_1", "start": 178890, "stop": 179498, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08529.16" ], "nt": "ATGAATGAATTAAACCAACCCTTACTTGCTATTATTAAAAATGTCGCTAAAACCAAAAACCTTTCTATAGAAGAGGTGGTTTTTTGTTTGAAAACAGCTTTAGAACAAGCCTATAAAAAACACCTTAACTTTGTTAATGTTGAAGTTAACATTAACTTTGATAAGGGGATTATTAATGTTGAACAACTCTTTAATGTTGTTAGTGATGACAATGAAGATTATGATGACTTTCTTGAAATCCCTTTACAAGCAGCTAACAAAATAAACAGTTCATTGCAATTAGGTGATGTGTTGCGTAAACCAATCCCCTTAAAAAACATTAGTAGTGATCTTATCAATAAGATGATTGCTATCTTTAACCAAAAGATTAGTGAAACAAACTTTAAAGCAGTAATGAGTGAGTTTAGTAGTGAGGTTGGGGAAGTGATTGAAGCGAAAGTTGAAGATATTGATACTAACAAAGAAGGTGGTTTAAAGGGTTATATTATTAACCTTGAAACTACAAAGGGTTATATCTCCAAGCGGGAATTGTCAAAAAGGGAGCGCTTAGAGATAGGTAAAAAATACCTCTTTGTTATCAAAGAAATCCAACGGCAAGCATCGTTATGA", "aa": "MNELNQPLLAIIKNVAKTKNLSIEEVVFCLKTALEQAYKKHLNFVNVEVNINFDKGIINVEQLFNVVSDDNEDYDDFLEIPLQAANKINSSLQLGDVLRKPIPLKNISSDLINKMIAIFNQKISETNFKAVMSEFSSEVGEVIEAKVEDIDTNKEGGLKGYIINLETTKGYISKRELSKRERLEIGKKYLFVIKEIQRQASL", "aa_hexdigest": "be62998bc0bcf646831b59284ab32100", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08529.16", "name": "NusA_N", "length": 121, "aa_cov": 0.5891089108910891, "hmm_cov": 0.9504132231404959, "evalue": 2.534924546496709e-21, "score": 71.26520538330078, "start": 8, "stop": 126 } ], "seq_stats": { "molecular_weight": 22966.19210000001, "isoelectric_point": 5.441731071472168 }, "id": "KMADJIBOEG_358", "locus": "GCA000292445_00322" }, { "type": "cds", "sequence": "contig_1", "start": 179541, "stop": 179930, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00013.35", "PFAM:PF13184.12" ], "nt": "TTGTTAACTTCAAATACTCCAGAAATTGAAAATGGTACGATTGTAATCAAAAAGATTGAACGTTCCCCAGGAGTGAAATCAAAGATAGCAGTTATCTCCAATGATCCTGCAGTGGACCCAGTTGCTGCTATCTTAGGACCTAAGGGTGAGAAGATTAGGGGGATTAGTGAGGAATTTAATGGTGAGATTATTGACATTGTCTTTTGGAATGAAGACAAGTTAAAGTTCTTAATTAATGCCATTTTACCTGCAGAAGTCATTGGTTATAACATCTTGCAGGATGATGAGCGTGATACTAGTATTGAAGTTGTTGTACCTGCAAACCAAATTGCTAATGTTTTTGGTTTTAAAGGTGTAAACATTAGGTTAATTAGTAATTTAACAGGTTGA", "aa": "MLTSNTPEIENGTIVIKKIERSPGVKSKIAVISNDPAVDPVAAILGPKGEKIRGISEEFNGEIIDIVFWNEDKLKFLINAILPAEVIGYNILQDDERDTSIEVVVPANQIANVFGFKGVNIRLISNLTG", "aa_hexdigest": "d48c40d913118e7b7dc7df232dcadd95", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00013.35", "name": "KH_1", "length": 27, "aa_cov": 0.20930232558139536, "hmm_cov": 0.9629629629629629, "evalue": 5.5374111100644824e-09, "score": 30.86907196044922, "start": 41, "stop": 67 }, { "id": "PF13184.12", "name": "KH_5", "length": 66, "aa_cov": 0.5116279069767442, "hmm_cov": 1.0, "evalue": 9.965585031600765e-23, "score": 75.27362823486328, "start": 23, "stop": 88 } ], "seq_stats": { "molecular_weight": 14041.997699999982, "isoelectric_point": 4.736416053771973 }, "id": "KMADJIBOEG_359", "locus": "GCA000292445_00323" }, { "type": "cds", "sequence": "contig_1", "start": 180012, "stop": 180410, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGGCATCAAAAAAAGAAGGGAAAAGATCATTAGTAATGATGCTACTGATAAAGTCTTTTACACCTCTAAAGACAATGTGATAGATGATGAGATTATTGTTGATTTAGCTAAAGATCTAATGGTTGATAATAAACAAAAACAACCTGAGCAAGTTGCAAAGCAAGTTGTTGAAAAATCACAATTAGAAAAACAAGTTACTCCAAAAGAAAAAGAGAAAGTTCAACCAAAAGCTAAGGTTCATTCTAATAGCCATTCCAAAAAACCAGCTAAACCTAATCAGATTTTTTCTATCACTGTTGATGCTAGTGATAAGAATCTTAAAAAAGATCAAGTTGATAATAACCAAACAAACCCCCAAACAAAACAAACATTTGATAGCTTTGATGATCTTTAA", "aa": "MFGIKKRREKIISNDATDKVFYTSKDNVIDDEIIVDLAKDLMVDNKQKQPEQVAKQVVEKSQLEKQVTPKEKEKVQPKAKVHSNSHSKKPAKPNQIFSITVDASDKNLKKDQVDNNQTNPQTKQTFDSFDDL", "aa_hexdigest": "0733e5c815ecc8572b3ab59a4550d869", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15126.898899999982, "isoelectric_point": 8.961314964294434 }, "id": "KMADJIBOEG_360", "locus": "GCA000292445_00324" }, { "type": "cds", "sequence": "contig_1", "start": 180410, "stop": 180682, "strand": "+", "frame": 2, "gene": "ylxR", "product": "putative protein MG141-1", "db_xrefs": [ "COG:AJ", "COG:COG2740", "RefSeq:WP_009885828.1", "SO:0001217", "UniParc:UPI0000557DAE", "UniRef:UniRef100_Q2MHT0", "UniRef:UniRef50_Q2MHT0", "UniRef:UniRef90_Q2MHT0" ], "nt": "ATGCAACTAATAACAAGACTTTGTTTATTAACAAGAAAACATTTTGTTAAAAGAGAACTTTTACGTCTTGTAAAATTAGACAACCAACTTGAAATTGATCTTAATCAAAATCTCAAGGGCAGGGGTTATTATTTGAGTGTTTTTGGTTTAAAGCTAGATAAAAAACACCTCAAAGCTGTAGTTGAAAAACACCTTAAGGTTAGTTGTAATGATGCAAAGCTTACTGCAATGATTACCGCCTTACAACAATTAGCACAAGATGAAAAAAAATAG", "aa": "MQLITRLCLLTRKHFVKRELLRLVKLDNQLEIDLNQNLKGRGYYLSVFGLKLDKKHLKAVVEKHLKVSCNDAKLTAMITALQQLAQDEKK", "aa_hexdigest": "99d2c3eec803f3ec69b4cb1a48b15476", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000557DAE", "ncbi_nrp_id": "WP_009885828.1", "uniref100_id": "UniRef100_Q2MHT0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000557DAE", "RefSeq:WP_009885828.1", "UniRef:UniRef100_Q2MHT0" ] }, "ips": { "uniref100_id": "UniRef100_Q2MHT0", "uniref90_id": "UniRef90_Q2MHT0" }, "psc": { "uniref90_id": "UniRef90_Q2MHT0", "gene": "YlxR", "product": "Uncharacterized protein MG141.1", "uniref50_id": "UniRef50_Q2MHT0", "cog_id": "COG2740", "cog_category": "AJ" }, "pscc": { "uniref50_id": "UniRef50_Q2MHT0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q2MHT0" ], "product": "Uncharacterized protein MG141.1" }, "genes": [ "ylxR" ], "id": "KMADJIBOEG_361", "locus": "GCA000292445_00325" }, { "type": "cds", "sequence": "contig_1", "start": 180875, "stop": 182266, "strand": "+", "frame": 2, "gene": "infB", "product": "translation initiation factor IF-2", "db_xrefs": [ "COG:COG0532", "COG:J", "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0006413", "KEGG:K02519", "SO:0001217", "UniRef:UniRef50_P47388", "UniRef:UniRef90_P47388" ], "nt": "TTGGCACTAACAGTTAATTCAGTTTTAACAAATGAACAGTTAGCAGATGCATGTGTTAACTTTGGGTTTGACTTTAAGATGGAAACTGAAGTTACCCATGAAAATATTGTAGCTAACATCCAGTTTGAAGATAGTGATGATTTATTGCAACCAAGACCACCTATTGTTACTATCATGGGTCATGTTGACCATGGTAAAACTTCGCTTTTAGACACAATTAGAAAAACTAATGTAACTGCTAAGGAGTTTGGCGGAATTACCCAAAAAATTGGTGCTTATCAAGTGAAAAATCACCAAAATAAAACGATTACTTTTATTGATACTCCTGGGCATGAAGCATTTACTTTAATGCGTGCAAGGGGTGCAAAAGTAACTGATATTGTGGTGTTGGTTGTGGCAGCGGATGATGGGATTAAAAAGCAAACAGAGGAAGCAATTAGCCATGCTAAGAGTGCTAACACTCCTATCATTGTTTTTATTAACAAGATGGATAAACCAACTGCTAACCCTGATCTGGTGATCCAACAACTCAATAAGTTTGATTTAGTTCCTGAGGCTTGGGGTGGGAAAACTATCTTTGTAATGGGTAGTGCTTTAACTGGTCAAGGGATTAATGAGTTGCTTGATAATATCTTGTTGCTAGGGGAAGTGGAGGGTTATCAAGCTAACTATAATGCCCATTCATCTGGTTATGCAATTGAAGTACAAACTTCAAAGGGACTTGGCCCTATTGCCAATGTCATTGTAAAAAGGGGTACTTTAAAGTTAGGTGACATTGTGGTGTTAGGGCCTGCATATGGAAGAGTTAGAACGATGCATGATGAAAATGGTAATAGCTTAAAACAAGCAACCCCTTCAAAACCTGTGCAGATCTCAGGGTTTGACATTATGCCTGTTGCTGGGGAAAAGTTCATTGTTTTTGATGATGAGAAGGATGCAAAGTTAATTGCTAACAAGTTTAAAGAACAACAAAAACAAAAAGCTAACAACTTAACAGTTAACCAAACCTTGAAAGAACAGATTAAAAACAAGGAAATTAAGGTATTAAATTTGATCTTTAAAGCAGATAGTGATGGTTCATTACAAGCTATTAAACAAGCAGTTGAAAACATTAATGTTGCTAAGATCTCACTTAGTATCATCCATGCTGCAGTGGGGCAGATATCAGAGAGTGATATTATGCTAGCAAAAGCATCAGGGGCTTTATTGTTTAGTTTAAACTTAGGTTTGAGTCAAACTGTAAAAAACATTGCTAGTTTACAAGGGGTAAAATTAGAAGTTCACTACCATATCCCTAAACTAGCAGAGGAGATTGAAAACATCTTAAAAGGTCAATTAGATCCTGTTTATGAAGAGATTGAAATAGGTAAAGCGGAAGTTTTACAACTCTGA", "aa": "MALTVNSVLTNEQLADACVNFGFDFKMETEVTHENIVANIQFEDSDDLLQPRPPIVTIMGHVDHGKTSLLDTIRKTNVTAKEFGGITQKIGAYQVKNHQNKTITFIDTPGHEAFTLMRARGAKVTDIVVLVVAADDGIKKQTEEAISHAKSANTPIIVFINKMDKPTANPDLVIQQLNKFDLVPEAWGGKTIFVMGSALTGQGINELLDNILLLGEVEGYQANYNAHSSGYAIEVQTSKGLGPIANVIVKRGTLKLGDIVVLGPAYGRVRTMHDENGNSLKQATPSKPVQISGFDIMPVAGEKFIVFDDEKDAKLIANKFKEQQKQKANNLTVNQTLKEQIKNKEIKVLNLIFKADSDGSLQAIKQAVENINVAKISLSIIHAAVGQISESDIMLAKASGALLFSLNLGLSQTVKNIASLQGVKLEVHYHIPKLAEEIENILKGQLDPVYEEIEIGKAEVLQL", "aa_hexdigest": "d915b210ec02153dc46cc0c0b87fbe1d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000B556DBD", "query_cov": 0.9460043196544277, "subject_cov": 0.8358778625954199, "identity": 0.502, "score": 395.0, "evalue": 2.99e-130, "valid": false, "gene": "infB", "product": "translation initiation factor IF-2", "uniref50_id": "UniRef50_A0A6L4YBC2", "kegg_orthology_id": "K02519", "go_ids": [ "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006413" ] }, "genes": [ "infB" ], "truncated": "both", "pseudogene": { "start": 180669, "stop": 182527, "observations": { "deletion": [ 180702 ], "stop": [ 182265 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47388", "query_cov": 1.0, "subject_cov": 0.7479806138933764, "identity": 0.996, "score": 883.0, "evalue": 0.0, "gene_start": 1, "gene_end": 463, "reference_start": 70, "reference_end": 532, "reference_sequence": "MKKNRAFNQVKKTKFDGRIKTSAKHQLRNVKTGVKDGVFIYKGPLTVSEFASKTNIAVANIIKHFFLNGLALTVNSVLTNEQLADACVNFGFDFKMETEVTHENIVANIQFEDSDDLLQPRPPIVTIMGHVDHGKTSLLDTIRKTNVTAKEFGGITQKIGAYQVKNHQNKTITFIDTPGHEAFTLMRARGAKVTDIVVLVVAADDGIKKQTEEAISHAKSANTPIIVFINKMDKPTANPDLVIQQLNKFDLVPEAWGGKTIFVMGSALTGQGINELLDNILLLGEVEGYQANYNAHSSGYAIEVQTSKGLGPIANVIVKRGTLKLGDIVVLGPAYGRVRTMHDENGNSLKQATPSKPVQISGFDIMPVAGEKFIVFDDEKDAKLIANKFKEQQKQKANNLTVNQTLKEQIKNKEIKILNLIFKADSDGSLQAIKQAVENINVAKISLSIIHAAVGQISESDIMLAKASGALLFSLNLGLSQTVKNIASLQGVKLEVHYHIPKLAEEIENILKGQLDPVYEEIEIGKAEVLQLWFHSKIGNIAGTIVKSGKIKRGNLCKLFRDKEIIFEGRIDSLKNEKTPVNLIETGKNCGIVINGCNDIKIGDIIVAYEKQIVKDGKL" }, "paralog": false, "identity": 0.9951534733441034, "score": 1195.0, "evalue": 0.0, "description": "Internal stop codon at 182265. Frameshift due to deletion around 180702.", "psc": { "uniref90_id": "UniRef90_P47388", "gene": "infB", "product": "translation initiation factor IF-2", "uniref50_id": "UniRef50_P47388", "cog_id": "COG0532", "cog_category": "J", "kegg_orthology_id": "K02519", "go_ids": [ "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0006413" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47388", "UniRef:UniRef50_P47388", "COG:COG0532", "COG:J", "KEGG:K02519", "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_P47388", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47388" ], "product": "Translation initiation factor IF-2" } }, "id": "KMADJIBOEG_362", "locus": "GCA000292445_00326" }, { "type": "cds", "sequence": "contig_1", "start": 182269, "stop": 182484, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATATCACCAATCTTAATATCATTGCAACCATTAATAACTATCCCACAATTCTTCCCTGTTTCTATTAAATTAACAGGCGTTTTTTCATTTTTTAAAGAGTCAATTCTACCTTCAAAGATAATCTCTTTATCTCTGAATAACTTACATAAACTCCCTCTTTTTATCTTTCCTGATTTAACAATGGTTCCTGCAATATTACCTATTTTAGAGTGA", "aa": "MISPILISLQPLITIPQFFPVSIKLTGVFSFFKESILPSKIISLSLNNLHKLPLFIFPDLTMVPAILPILE", "aa_hexdigest": "f6146627054f6b6847cfa6a38e0f6871", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7946.660499999993, "isoelectric_point": 8.213030433654787 }, "id": "KMADJIBOEG_363", "locus": "GCA000292445_00327" }, { "type": "cds", "sequence": "contig_1", "start": 182514, "stop": 182852, "strand": "+", "frame": 3, "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "db_xrefs": [ "COG:COG0858", "COG:J", "GO:0005829", "GO:0006364", "GO:0030490", "GO:0042254", "GO:0043024", "KEGG:K02834", "RefSeq:WP_009885829.1", "SO:0001217", "UniParc:UPI00001331AF", "UniRef:UniRef100_P47389", "UniRef:UniRef50_P47389", "UniRef:UniRef90_P47389" ], "nt": "ATGGCAAGCTATAGAAAACAACGGATTGAAAATGATATCATCCGCTTAATTAATCGCACGATTATTAATGAGATCTATGATCCTGTTGTTAAGTTAGGTCATGTTAGCCATGTGAAGTTATCAGCTGATTTTTTTCATGCAGTAGTTTATCTTGATTGTTATGATCGTAGTCAGATTCAAACTGTAGTTAATGCTTTTAAAAAGGCTCAGGGCGTTTTTAGTCAAATGTTAGCACAAAATTTGTACCTAGCTAAAAGTGTAAAACTCCACTTTGTGAAGGATGATGCAATTGACAATGCTTTGAAAATAGAACAGATAATTAACTCTTTGAAAAACTAA", "aa": "MASYRKQRIENDIIRLINRTIINEIYDPVVKLGHVSHVKLSADFFHAVVYLDCYDRSQIQTVVNAFKKAQGVFSQMLAQNLYLAKSVKLHFVKDDAIDNALKIEQIINSLKN", "aa_hexdigest": "4500bbaa521ddaacfdfd8700f355eab2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001331AF", "ncbi_nrp_id": "WP_009885829.1", "uniref100_id": "UniRef100_P47389", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001331AF", "RefSeq:WP_009885829.1", "UniRef:UniRef100_P47389" ] }, "ips": { "uniref100_id": "UniRef100_P47389", "uniref90_id": "UniRef90_P47389" }, "psc": { "uniref90_id": "UniRef90_P47389", "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "uniref50_id": "UniRef50_P47389", "cog_id": "COG0858", "cog_category": "J", "kegg_orthology_id": "K02834", "go_ids": [ "GO:0005829", "GO:0006364", "GO:0030490", "GO:0042254", "GO:0043024" ] }, "pscc": { "uniref50_id": "UniRef50_P47389", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47389" ], "product": "Ribosome-binding factor A" }, "genes": [ "rbfA" ], "id": "KMADJIBOEG_364", "locus": "GCA000292445_00328" }, { "type": "cds", "sequence": "contig_1", "start": 182877, "stop": 183305, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MG144", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47390", "UniRef:UniRef90_P47390" ], "nt": "ATGGACCCACAAAACAAGTCCCCAAAACCACCAGTTAAATCAACTAGATTGGTTGTCAAAAAACAACCAGCAGGTGTTGTTTTTCCCAAATTGAGTATTCCTGTTAATGATTTTGAAAAAACAGTTACTTTAACAAGAGCACAAAAAAAGGAAGCTAAATTTCTCAAAAAAGCCCAAAGGAAAGCTAATAAGCTTAATAATAAACAAGATAGTATCTTTTTCAATTCTGCTTCAGTAGGTGACACTAATAACACCATTCTTCCTCCTGGTGTTAAAAATCAAGCTGATAATAAAACTAACCGTTTTAGTAAATTTATTAGTTTTTTCACTTCTTCAAAAAACAAACAACCAGATGAAATAACAGAAAGGTTAGTTGATGATCCTACTGTTAAAAACCGTTTTAGTGCTTTTAATAAGAAGCTTATTTGA", "aa": "MDPQNKSPKPPVKSTRLVVKKQPAGVVFPKLSIPVNDFEKTVTLTRAQKKEAKFLKKAQRKANKLNNKQDSIFFNSASVGDTNNTILPPGVKNQADNKTNRFSKFISFFTSSKNKQPDEITERLVDDPTVKNRFSAFNKKLI", "aa_hexdigest": "aebcdc8f245760c6e36a607bbaa645bf", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 182877, "stop": 183608, "observations": { "mutation": [ 183303, 183477, 183522 ], "stop": [ 183303, 183477, 183522 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47390", "query_cov": 1.0, "subject_cov": 0.5071428571428571, "identity": 0.993, "score": 270.0, "evalue": 2.24e-89, "gene_start": 1, "gene_end": 142, "reference_start": 1, "reference_end": 142, "reference_sequence": "MDPQNKSPKPPVKSTRLVVKKQPAGVVFPKLSIPVNDFEKTVTLTRAQKKEAKLLKKAQRKANKLNNKQDSIFFNSASVGDTNNTILPPGVKNQADNKTNRFSKFISFFTSSKNKQPDEITERLVDDPTVKNRFSAFNKKLIWVLKDKKLRARAWKIVGYTNLVIVAFFAGLLAVMNKFITLPSVEYPAIALQLPINNALWGISIFVISIVTLPFWTMFILFLMGVKDVRTSRSIHYFIWIVLIINVVLLLVSCLLMIAAYAHLDGYNIWRNLESLNPNN" }, "paralog": false, "identity": 0.9794238683127572, "score": 461.0, "evalue": 3.69e-167, "description": "Internal stop codon at 183303, 183477, 183522. Nonsense mutation around 183303, 183477, 183522.", "psc": { "uniref90_id": "UniRef90_P47390", "product": "Uncharacterized protein MG144", "uniref50_id": "UniRef50_P47390", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47390", "UniRef:UniRef50_P47390", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47390", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47390" ], "product": "Uncharacterized protein MG144" } }, "id": "KMADJIBOEG_365", "locus": "GCA000292445_00329" }, { "type": "cds", "sequence": "contig_1", "start": 183372, "stop": 183479, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCATTTTTTGCTGGACTTTTAGCAGTGATGAATAAGTTCATCACCCTTTCCTCAGTTGAATATCCTGCTATTGCTTTACAACTCCCTATTAACAATGCATTATGA", "aa": "MAFFAGLLAVMNKFITLSSVEYPAIALQLPINNAL", "aa_hexdigest": "9d5085af04e692f4f40cff4bc8dfbbab", "start_type": "GTG", "rbs_motif": "TTATA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3782.5132000000003, "isoelectric_point": 5.752754020690918 }, "id": "KMADJIBOEG_366", "locus": "GCA000292445_00330" }, { "type": "cds", "sequence": "contig_1", "start": 183528, "stop": 183686, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATCTTGTTTTTAATGGGAGTAAAGGATGTGAGAACTTCGCGTTCTATCCATTATTTTATCTGGATAGTGTTAATTATTAATGTAGTTTTATTACTAGTTAGTTGCTTGTTGATGATTGCTGCTTATGCCCATCTTGATGGTTATAACATCTGA", "aa": "MFILFLMGVKDVRTSRSIHYFIWIVLIINVVLLLVSCLLMIAAYAHLDGYNI", "aa_hexdigest": "96b80d41985d832101b1e57399461bd2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6013.336699999999, "isoelectric_point": 7.959798622131348 }, "id": "KMADJIBOEG_367", "locus": "GCA000292445_00331" }, { "type": "cds", "sequence": "contig_1", "start": 183719, "stop": 184528, "strand": "+", "frame": 2, "gene": "ribF", "product": "riboflavin biosynthesis protein RibF", "db_xrefs": [ "COG:COG0196", "COG:H", "EC:2.7.1.26", "EC:2.7.7.2", "GO:0003919", "GO:0005524", "GO:0005737", "GO:0006747", "GO:0006771", "GO:0008531", "GO:0009231", "GO:0009398", "KEGG:K11753", "RefSeq:WP_009885831.1", "SO:0001217", "UniParc:UPI00001338E0", "UniRef:UniRef100_P47391", "UniRef:UniRef50_P47391", "UniRef:UniRef90_P47391" ], "nt": "ATGAAATCACTCTTTATTGGTTATTTTGATGGGTTACATCAAGGTCATCTATTTTTAAAGCAGAACAGTAAGTTTGAACCAATGGTGTTATTAATTGATAACCCACCTTTAAAACAAACCAACTGGCTTTATGATTTACAACAACGGGTTGCACAAATAAAAACTTACTTGAAAGCAACTGTAGAAGTATTTGATGTTGCCAAACATAACATGAATGCACTTAGTTTTTTTGAACAACAGATTAAAAGATTGAATTGTGATGAAATTATTGTTGGTACAGATTGGCATTTTGGTAATGATCATAAGGATGGGATCTGGTTAAAGAAACTGTTTAAAAATACTGTTATTGTTAATAAAACAAACCTATCAAGTAGTGTTATCCGTAACTATCTAACTAATAATGAACTTGAAAAAGCTAACCAACTTTTAGTGGAACCTTATTATAGAGTGGGCACAGTAGTACATGGTTTAAAAAAGGCAAGGTTGCTTGGTTTTCCAACTGCTAACATTGTTATGGATAACCACTTATTGACTTTAAATAAGGGGAGTTATATAGTAAGAGTTTTATTAAATAACCAAACTTTTTATGGGATTGGTTTTATTAGCCAAAAGGATCAGGATTTGGTGTGTGAAACCCATATCTTTAACTTTAATAATGAGATTTATGGTTCACTGGTCAAATTTACACTGTTAAAGTTCATTAGAACAATTAGTAAGTTTTCCAGTCAAGCAGCTTTGCAAAAAGCAATTCAAAGTGATGCTAACTTTGCTTTAAAGTGGTTGGAAAACCAAAATTTAGATAAAATTTAA", "aa": "MKSLFIGYFDGLHQGHLFLKQNSKFEPMVLLIDNPPLKQTNWLYDLQQRVAQIKTYLKATVEVFDVAKHNMNALSFFEQQIKRLNCDEIIVGTDWHFGNDHKDGIWLKKLFKNTVIVNKTNLSSSVIRNYLTNNELEKANQLLVEPYYRVGTVVHGLKKARLLGFPTANIVMDNHLLTLNKGSYIVRVLLNNQTFYGIGFISQKDQDLVCETHIFNFNNEIYGSLVKFTLLKFIRTISKFSSQAALQKAIQSDANFALKWLENQNLDKI", "aa_hexdigest": "1b90d804a9a9d34cfb6cb35101a8d0e9", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001338E0", "ncbi_nrp_id": "WP_009885831.1", "uniref100_id": "UniRef100_P47391", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001338E0", "RefSeq:WP_009885831.1", "UniRef:UniRef100_P47391" ] }, "ips": { "uniref100_id": "UniRef100_P47391", "uniref90_id": "UniRef90_P47391" }, "psc": { "uniref90_id": "UniRef90_P47391", "gene": "ribF", "product": "riboflavin biosynthesis protein RibF", "ec_ids": [ "2.7.1.26", "2.7.7.2" ], "uniref50_id": "UniRef50_P47391", "cog_id": "COG0196", "cog_category": "H", "kegg_orthology_id": "K11753", "go_ids": [ "GO:0003919", "GO:0005524", "GO:0005737", "GO:0006747", "GO:0006771", "GO:0008531", "GO:0009231", "GO:0009398" ] }, "pscc": { "uniref50_id": "UniRef50_P47391", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47391" ], "product": "Bifunctional riboflavin kinase/FMN adenylyltransferase" }, "genes": [ "ribF" ], "id": "KMADJIBOEG_368", "locus": "GCA000292445_00332" }, { "type": "cds", "sequence": "contig_1", "start": 184541, "stop": 184666, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACAGTGCCCCCAGTGGTTTAACTTTAACTGTTATTATCCTTAGCATCATTCTGCTTGCTTTTATCAGCACAGTTGTATCAGCTTATGAAACAGCAATCACTTCTTTAACCCCTTACAGGTGA", "aa": "MDSAPSGLTLTVIILSIILLAFISTVVSAYETAITSLTPYR", "aa_hexdigest": "aa09da1cac1cae4958c9e0a811a6736d", "start_type": "ATG", "rbs_motif": "ATTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4343.083299999999, "isoelectric_point": 4.369065666198731 }, "id": "KMADJIBOEG_369", "locus": "GCA000292445_00333" }, { "type": "cds", "sequence": "contig_1", "start": 184790, "stop": 184990, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTCTAACATCCTTTTTTTAGCACTAGAACAAACAATTAAAAATGAGCTTTTATCAAGTGTTTTAAATTTGGTAGTTAGTGGGGTTTTAATCGTCTCTTTTTGTGAAATTCTACCCAAAACTTTGGGCAGAATTAATGTGATTAGAACCCTGGTTCTATTTGCTTATTTGGTTTATTTTTTTTATCTGATCTTTTGA", "aa": "MVSNILFLALEQTIKNELLSSVLNLVVSGVLIVSFCEILPKTLGRINVIRTLVLFAYLVYFFYLIF", "aa_hexdigest": "704535b5478442b3e2e21541772bb6e1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7526.074599999995, "isoelectric_point": 7.899520683288574 }, "id": "KMADJIBOEG_370", "locus": "GCA000292445_00334" }, { "type": "cds", "sequence": "contig_1", "start": 185186, "stop": 185581, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00571.33" ], "nt": "GTGGCTTATTGTTATCTTAATGACAGTATTAATTTGATTGCCAAGCAGTTTTTACAAGGGCAGTTTTCCAGAATGCCAGTAGTAGATAAAACTACTAATAAGATAGTTGGTTTTATCCATTTAAAGGATTTTTTTACAGCTAAAGAAGCAAACCCTAAGTCACTTGATTTAAACCAGTTGCTTTATCCAGTTGTTTTAGTTCAAGATTCCACCCCCATCAAACAAGCACTAAGACAGATGCGTTTAAACAGAGCACATTTAGCAGTTGTTAATGACAAACATGAAAAAACAATAGGGATTGTTTCTATGGAAGATATTATTGAAGAGTTGGTGGGTGAAATCTATGATGAACATGATGATATCCAACCGATCCAAGTATTGGATGAAAATGTTTGA", "aa": "MAYCYLNDSINLIAKQFLQGQFSRMPVVDKTTNKIVGFIHLKDFFTAKEANPKSLDLNQLLYPVVLVQDSTPIKQALRQMRLNRAHLAVVNDKHEKTIGIVSMEDIIEELVGEIYDEHDDIQPIQVLDENV", "aa_hexdigest": "592192f3d29fcbca1e16bb514e5772fc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00571.33", "name": "CBS", "length": 50, "aa_cov": 0.37404580152671757, "hmm_cov": 0.98, "evalue": 1.2698717768923147e-19, "score": 65.50303649902344, "start": 63, "stop": 111 } ], "seq_stats": { "molecular_weight": 15010.11379999999, "isoelectric_point": 5.084725379943847 }, "id": "KMADJIBOEG_371", "locus": "GCA000292445_00335" }, { "type": "cds", "sequence": "contig_1", "start": 185639, "stop": 185815, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAAAATCAAAAAATATTACTATCCAGCATTATCTCGCTTCACTAGATAATGATAGTTTTGCTTGCCAAAATAAGCTTGACACTCCCTTATTTAGTGTTGAAGTGATAGCTGATAGCGAAGATAAAACCAAAATTCTTTACGAAATTAGAAAAAAGAGCGATGTTATTGCTTAG", "aa": "MVKSKNITIQHYLASLDNDSFACQNKLDTPLFSVEVIADSEDKTKILYEIRKKSDVIA", "aa_hexdigest": "623a728d4b378e88c1b6ba185ec7bab2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6588.471799999999, "isoelectric_point": 5.628106117248534 }, "id": "KMADJIBOEG_372", "locus": "GCA000292445_00336" }, { "type": "cds", "sequence": "contig_1", "start": 185802, "stop": 186455, "strand": "+", "frame": 3, "gene": null, "product": "MPN160 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47393", "UniRef:UniRef90_P47393" ], "nt": "ATGTTATTGCTTAGAGCATTATTTTTAGAGTTGAAAACCAACAAAAATTGTAAAGCTTTACTTTTGTTATTAATCCTCTTACTAGTTGGTTTAACCTTGATAATCTATGGGATTGTCTTATTTTCCACTGAAGGGGTAATTGACCATGGTGATCATAACCACTTAAGAGCAAGGTTTCAACTCACTTTAGAGGAGATTATTGTTTTTGTTGTTGGTAGTATTATCTTGTTTTTTACTTTAGCTAGCTTTTGTGTAAGTTGCTTTATGTTAATGAGAAGTCCTAAGCAAAAACAGCTAGAGGTTGATCATGCTAATAAAACTAATTTAAAACCAAAAGCAATAGTTAATTGTGATCTTTTTCAGTTGGGTGATCATTGTGTGTTCACATTTAAAAAACTTAGCTTTAAACAACGGTTTAAGCAAGATTTTTTTGCTAGAAGTAAGTTTTCGTTTCGCAGTGAACTGTATCGTCTTTGTTTGGTGGGAGTGTCAATTGCTCTCAATTTAGCATTAAGTTTAATTGAAATTCCTGGGATAGTTTTACCTTGGGGTAGTTCGATCCAGTTCCGTTTTTTTAATACTGCTATCTTGTTTATTGCTGTTAGGTTTGTGGGGTTATTATCTACTTCTTTAGTGGCTTTAATCACCCCCTGA", "aa": "MLLLRALFLELKTNKNCKALLLLLILLLVGLTLIIYGIVLFSTEGVIDHGDHNHLRARFQLTLEEIIVFVVGSIILFFTLASFCVSCFMLMRSPKQKQLEVDHANKTNLKPKAIVNCDLFQLGDHCVFTFKKLSFKQRFKQDFFARSKFSFRSELYRLCLVGVSIALNLALSLIEIPGIVLPWGSSIQFRFFNTAILFIAVRFVGLLSTSLVALITP", "aa_hexdigest": "d53e55dfbe2330de95aac7494885ed28", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 185802, "stop": 186757, "observations": { "deletion": [ 186654 ], "mutation": [ 186453, 186531, 186642 ], "stop": [ 186453, 186531, 186642 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47393", "query_cov": 1.0, "subject_cov": 0.5786666666666667, "identity": 0.982, "score": 391.0, "evalue": 1.48e-134, "gene_start": 1, "gene_end": 217, "reference_start": 1, "reference_end": 217, "reference_sequence": "MLLLRALFLELKTNKNCKALLLLLIPLLVGLTLIIYGIVLFSTEGVIDHGDHNHLRARFQLTLEEIIVFVVGSIILFFTLASFCVSCFMLMRSPKQKQLEVDHANKTNLKPKAIVNCDLFQLGDYCVFTFKKLSFKQRFKQDFFARSKFSFRSELYRLCLVGVLIALNLALSLIEIPGIVLPWGSSIQFRFFNTAILFIAVRLVGLLSTSLVALITPWLHLLIHPIHTPISSLFYMVNDFLVLWIFYFFFFHLFKAEVIQTTTVVDNKPFSQLVNTKKTKWTKFFSLLVISFLCGFIEGLGFYFGYFLILGNVSSLGLKIYYDGLQQRDLINSSNVLFFLMTTTAIFSIKYIFEMLFFFSVEKNVVNIANHFGLY" }, "paralog": false, "identity": 0.9716981132075472, "score": 596.0, "evalue": 5.73e-218, "description": "Internal stop codon at 186453, 186531, 186642. Frameshift due to deletion around 186654. Nonsense mutation around 186453, 186531, 186642.", "psc": { "uniref90_id": "UniRef90_P47393", "product": "MPN160 family protein", "uniref50_id": "UniRef50_P47393", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47393", "UniRef:UniRef50_P47393", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47393", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47393" ], "product": "Uncharacterized protein MG147" } }, "id": "KMADJIBOEG_373", "locus": "GCA000292445_00337" }, { "type": "cds", "sequence": "contig_1", "start": 186641, "stop": 186928, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAACAAAGTTTTTTCCTTACTTGCAATTAGCTTTTTGTGTGGCTTTATTGAAGGGTTAGGTTTTTACTTTGGTTATTTTTTAATCCTTGGTAATGTTTCTAGTTTAGGATTGAAGATCTATTATGATGGGTTACAACAACGTGATCTGATTAACAGTAGTAATGTTTTGTTCTTTTTAATGACAACAACTGCTATCTTCAGTATTAAATACATCTTTGAGATGTTATTTTTCTTTAGTGTTGAAAAGAATGTAGTTAATATTGCTAACCACTTTGGTTTGTATTAA", "aa": "MNKVFSLLAISFLCGFIEGLGFYFGYFLILGNVSSLGLKIYYDGLQQRDLINSSNVLFFLMTTTAIFSIKYIFEMLFFFSVEKNVVNIANHFGLY", "aa_hexdigest": "1aa6cb0f8dea1eccc693273471673138", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10899.711199999992, "isoelectric_point": 6.521728706359863 }, "id": "KMADJIBOEG_374", "locus": "GCA000292445_00338" }, { "type": "cds", "sequence": "contig_1", "start": 187203, "stop": 187457, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAATGACAAAGAGCAAGCATTGTTGTTAAAAACCAAATTAGAATTACAAAACCAGTTTCAAAAGCAGATAGAGAACTATATCAATGAGATTAACAAGCTCAAGTTAACCAATAAAGAGCTTGAAATTACTAACCAAAAACAACTAGAAAGCTCACTTAAACTACAGCGAAACGAATTTGAAGAGAAAATCAACCAGCAAAACTTAACGATTGAAAAACTGAAAATTCAACAAGCAAGAAGTAGTATCTGA", "aa": "MLNDKEQALLLKTKLELQNQFQKQIENYINEINKLKLTNKELEITNQKQLESSLKLQRNEFEEKINQQNLTIEKLKIQQARSSI", "aa_hexdigest": "b1080aa3ab92b907a29c4a22b918f316", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10055.436699999993, "isoelectric_point": 8.925728416442873 }, "id": "KMADJIBOEG_375", "locus": "GCA000292445_00339" }, { "type": "cds", "sequence": "contig_1", "start": 187671, "stop": 188135, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGTGAGTTTAATGATAGTAAGCATAAATCAAAAAACAAAGATCATATTAGCAAACTGGTCAGGGATGCTAAACGTGCTAACTGCAAGTATGCTTTTTTAATTAGTGAACTTGAACTGGAGACTGAAAATGACATCCAAGTGCGCTTAATGCCAACATTGGAAAGTGGTGTTGAAGTCTATCTAGTTAGACCAATGTTTTTTATCTTAATGCTTAAACTTTTCTATAAGTTAGCTAAGAAGTTGTTTGCCCTTAACCGTTTTCAATCAGTTGAACTAATTGATAAAAATAAGTTAAATGAACAGTTTAAACAGTTGAAAGATAATTTTTTAACCAAAACCTTTTTAGAGATTGAAAAAGTGTGTAAAAGTAACTTAGTTGATATTGAAACACTTGAAAAAGCAGTGGTGAAACTAAGGGTTAGAAACGAACGCGTTTTAGATCAGTTACTTAATAAATGA", "aa": "MKSEFNDSKHKSKNKDHISKLVRDAKRANCKYAFLISELELETENDIQVRLMPTLESGVEVYLVRPMFFILMLKLFYKLAKKLFALNRFQSVELIDKNKLNEQFKQLKDNFLTKTFLEIEKVCKSNLVDIETLEKAVVKLRVRNERVLDQLLNK", "aa_hexdigest": "4ef5e11ec56d49368a7dc844cc105c97", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18255.3119, "isoelectric_point": 9.450823402404787 }, "id": "KMADJIBOEG_376", "locus": "GCA000292445_00340" }, { "type": "cds", "sequence": "contig_1", "start": 188271, "stop": 188981, "strand": "+", "frame": 3, "gene": null, "product": "MPN162 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47395", "UniRef:UniRef90_P47395" ], "nt": "ATGAAATTATACCGATCTTTAAAAGCAGCCCTGTTACCAGGGATATGCACTAGCATTTTACTTGCTAGTTGTGCTTCAACAAATACTTATCAAGACCAAAGGAATGCCTTGATTAGTTTGGCTTCTAATCGTGATACTTTAATTGCGAATGCTAAAAAATCCAAAGAAGAAGTGCAAAAAGAAGTTACCAAAATGAATAGTAGTACTTCATCAATGATGACAGCTACCCAAAGTGTTGCAATTACAACACATCAAACTACTGCTCAAAACAGTTCCAAATATGATTTAGACAAGCTTTTTAAGGATTACATCCTTTATGTTGTTGATAATTTTTCAGGACTTGTTTTTAAAAGAACTGGCGATCATAGGATCCAGTTAATCGATAAGGATAAAGAGATTTTGGATGGTGGTAATCTAACTAAACATACCCACCATGATCATAACCATATGCATGAACATGAACATGAAGAACACCATGATGAAGAAGAGACAGAAGTAGTTGGCAGAGTACTCTCTTTTACTAATGGCATCTTTCTAGTGATTGACTATAAAAAAGACTCTGAAAGAAAAAATATGAGTGATTCAACTACTATGATGCACCAACACCACCATGAAGCTGAAGAACATAAAGAGGAACGTAAGCTCTCTTTAAACTTAAAAGCATACAAATTTAATACCCCTTTTAACATTAGTGAGTTTATTAGTGCTTGA", "aa": "MKLYRSLKAALLPGICTSILLASCASTNTYQDQRNALISLASNRDTLIANAKKSKEEVQKEVTKMNSSTSSMMTATQSVAITTHQTTAQNSSKYDLDKLFKDYILYVVDNFSGLVFKRTGDHRIQLIDKDKEILDGGNLTKHTHHDHNHMHEHEHEEHHDEEETEVVGRVLSFTNGIFLVIDYKKDSERKNMSDSTTMMHQHHHEAEEHKEERKLSLNLKAYKFNTPFNISEFISA", "aa_hexdigest": "1367a811991ca67e0beaa5b3d79b6b85", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47395", "query_cov": 1.0127118644067796, "subject_cov": 0.8505338078291815, "identity": 0.958, "score": 369.0, "evalue": 4.09e-127, "valid": true, "product": "MPN162 family protein", "uniref50_id": "UniRef50_P47395", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47395", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47395" ], "product": "Uncharacterized lipoprotein MG149" }, "genes": [], "id": "KMADJIBOEG_377", "locus": "GCA000292445_00341" }, { "type": "cds", "sequence": "contig_1", "start": 189201, "stop": 189293, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAACAAAACCCTGATCGTTTAAAAAAAGATAGGGAACTTATTTATGCAATTGTTACAGCTAAAGGTATCATTAGCCGTTTCTTTTGA", "aa": "MEQQNPDRLKKDRELIYAIVTAKGIISRFF", "aa_hexdigest": "a2c9ce7421d5ee46c6e43dfc0bd9f152", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3581.1499999999996, "isoelectric_point": 9.518902015686034 }, "id": "KMADJIBOEG_378", "locus": "GCA000292445_00342" }, { "type": "cds", "sequence": "contig_1", "start": 189456, "stop": 189611, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_009885834.1", "SO:0001217", "UniParc:UPI0001B417EB", "UniRef:UniRef100_UPI0001B417EB", "UniRef:UniRef50_UPI0001B417EB", "UniRef:UniRef90_UPI0001B417EB" ], "nt": "TTGTTTATCTATTTTAAGGGCCAGAAAGCAATTGAAAACAACTGTAAGTACCTGTTAACAATCCTTGATATTAAGAGTGATGAACTGTTACCTTTTTCGCTTTTAGGTAGTTTAAGAAAAGGTTATATGCTAGATGAAATGCTCTTAGAACAGTAA", "aa": "MFIYFKGQKAIENNCKYLLTILDIKSDELLPFSLLGSLRKGYMLDEMLLEQ", "aa_hexdigest": "9c56816882705e57cdcda1dd5e060d34", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001B417EB", "ncbi_nrp_id": "WP_009885834.1", "uniref100_id": "UniRef100_UPI0001B417EB", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001B417EB", "RefSeq:WP_009885834.1", "UniRef:UniRef100_UPI0001B417EB" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001B417EB", "uniref90_id": "UniRef90_UPI0001B417EB" }, "psc": { "uniref90_id": "UniRef90_UPI0001B417EB", "uniref50_id": "UniRef50_UPI0001B417EB" }, "pscc": { "uniref50_id": "UniRef50_UPI0001B417EB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B417EB" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5988.062999999997, "isoelectric_point": 5.095922660827636 }, "id": "KMADJIBOEG_379", "locus": "GCA000292445_00343" }, { "type": "cds", "sequence": "contig_1", "start": 189701, "stop": 190021, "strand": "+", "frame": 2, "gene": "rpsJ", "product": "30S ribosomal protein S10", "db_xrefs": [ "COG:COG0051", "COG:J", "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0022627", "RefSeq:WP_009885835.1", "SO:0001217", "UniParc:UPI0000134BB3", "UniRef:UniRef100_P47396", "UniRef:UniRef50_O52331", "UniRef:UniRef90_P75581" ], "nt": "ATGAATAGCGCTGTAAAATATCCTGAGCTGAAGATCAAACTTGAGTCTTATGATAGCACGCTTTTAGATCTCACTATTAAAAAGATAGTTGAGGTTGTAAAGGGTGTGAACATTAAGATTAAAGGTCCTTTACCTTTGCCTACTAAAAAGGAAGTGATCACCATTATCCGCTCTCCCCATGTTGATAAAGCATCCAGAGAGCAGTTTGAAAAAAATACCCACAAGCGCTTAATGATTCTTGTTGATGTTAATCAAGGAGGGATTGATAGTTTAAAAAAGATTAAGATCCCAGTTGGGGTTACACTGCGTTTTTCAAAATAG", "aa": "MNSAVKYPELKIKLESYDSTLLDLTIKKIVEVVKGVNIKIKGPLPLPTKKEVITIIRSPHVDKASREQFEKNTHKRLMILVDVNQGGIDSLKKIKIPVGVTLRFSK", "aa_hexdigest": "81c51a3a40e700b1bc8088cf9449d925", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134BB3", "ncbi_nrp_id": "WP_009885835.1", "uniref100_id": "UniRef100_P47396", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134BB3", "RefSeq:WP_009885835.1", "UniRef:UniRef100_P47396" ] }, "ips": { "uniref100_id": "UniRef100_P47396", "uniref90_id": "UniRef90_P75581" }, "psc": { "uniref90_id": "UniRef90_P75581", "gene": "rpsJ", "product": "30S ribosomal protein S10", "uniref50_id": "UniRef50_O52331", "cog_id": "COG0051", "cog_category": "J", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_O52331", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52331" ], "product": "Small ribosomal subunit protein uS10" }, "genes": [ "rpsJ" ], "id": "KMADJIBOEG_380", "locus": "GCA000292445_00344" }, { "type": "cds", "sequence": "contig_1", "start": 190025, "stop": 190402, "strand": "+", "frame": 2, "gene": "rplC", "product": "50S ribosomal protein L3", "db_xrefs": [ "COG:COG0087", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "KEGG:K02906", "SO:0001217", "UniRef:UniRef50_P75580", "UniRef:UniRef90_P47397" ], "nt": "ATGGATGTAAGGGGAATATTTGGTGTTAAAGTAGGGATGAGTCAGATCTTTACTGAGCAAAATGAGTGCTTACCTATCACCATTGTTTATTGTGAAGCTAATCAGGTGGCTGGGATTAAAACGATTGCTAAAGATAATTACAACGCCACTCTATTAAGCTTTCAAACTGTTGATGAAAAACAACTTAACAAACCTAAACAAGGGTTCTTTTCCAAACTTAAACTAGAACCTCATAAATATCTGAGGGAAATCAGAAAGATGCAAGGGTTTGAGTTAGGTAAGAAGATCACCCCCCAGGAGTTGTTTAAGATAGGTGAATATGTTGATGTCACTTCACTCACCAAAGGTAGGGGTTTTACAGGAGCGATTAAAAGGTGA", "aa": "MDVRGIFGVKVGMSQIFTEQNECLPITIVYCEANQVAGIKTIAKDNYNATLLSFQTVDEKQLNKPKQGFFSKLKLEPHKYLREIRKMQGFELGKKITPQELFKIGEYVDVTSLTKGRGFTGAIKR", "aa_hexdigest": "ab91b20669b8ba3d57eb2675780df1de", "start_type": "ATG", "rbs_motif": null, "genes": [ "rplC" ], "truncated": "3-prime", "pseudogene": { "start": 190025, "stop": 190705, "observations": { "mutation": [ 190400 ], "stop": [ 190400 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47397", "query_cov": 1.0, "subject_cov": 0.48638132295719844, "identity": 1.0, "score": 250.0, "evalue": 2.1e-82, "gene_start": 1, "gene_end": 125, "reference_start": 1, "reference_end": 125, "reference_sequence": "MDVRGIFGVKVGMSQIFTEQNECLPITIVYCEANQVAGIKTIAKDNYNATLLSFQTVDEKQLNKPKQGFFSKLKLEPHKYLREIRKMQGFELGKKITPQELFKIGEYVDVTSLTKGRGFTGAIKRWNFKIGPLGHGAGYPHRFQGSVQAGRGGSSAQRVFKGKKMSGHYGHEQVTIQNLFIVGFDEINKLVLVSGAIAGPEGGIVLIKTAKKKTGKIKDIKLAVQTVKAPQLKAPKKQKTKVETNQVNPKIEEEKTK" }, "paralog": false, "identity": 0.9911504424778761, "score": 447.0, "evalue": 1.77e-162, "description": "Internal stop codon at 190400. Nonsense mutation around 190400.", "psc": { "uniref90_id": "UniRef90_P47397", "gene": "rplC", "product": "50S ribosomal protein L3", "uniref50_id": "UniRef50_P75580", "cog_id": "COG0087", "cog_category": "J", "kegg_orthology_id": "K02906", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47397", "UniRef:UniRef50_P75580", "COG:COG0087", "COG:J", "KEGG:K02906", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P75580", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75580" ], "product": "Large ribosomal subunit protein uL3" } }, "id": "KMADJIBOEG_381", "locus": "GCA000292445_00345" }, { "type": "cds", "sequence": "contig_1", "start": 190421, "stop": 190798, "strand": "+", "frame": 2, "gene": "rplC", "product": "50S ribosomal protein L3", "db_xrefs": [ "COG:COG0087", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "KEGG:K02906", "SO:0001217", "UniRef:UniRef50_P75580", "UniRef:UniRef90_P47397" ], "nt": "TTGGGTCATGGGGCGGGTTATCCCCACCGCTTTCAGGGTTCTGTGCAAGCAGGTAGAGGTGGTAGTAGTGCGCAGCGTGTTTTTAAGGGTAAGAAGATGTCTGGGCATTATGGTCATGAACAAGTTACGATCCAAAACCTCTTTATTGTTGGCTTTGATGAAATCAATAAGTTAGTGTTAGTTTCAGGCGCAATTGCTGGTCCTGAGGGTGGGATTGTTTTAATTAAAACTGCAAGAAAGAAAACTGGCAAGATAAAAGATATAAAGTTAGCAGTACAAACTGTTAAAGCCCCACAACTAAAAGCACCAAAAAAGCAGAAAACTAAGGTTGAAACCAACCAGGTTAACCCAAAAATTGAAGAAGAGAAAACTAAGTAA", "aa": "MGHGAGYPHRFQGSVQAGRGGSSAQRVFKGKKMSGHYGHEQVTIQNLFIVGFDEINKLVLVSGAIAGPEGGIVLIKTARKKTGKIKDIKLAVQTVKAPQLKAPKKQKTKVETNQVNPKIEEEKTK", "aa_hexdigest": "def43abacf93a1a9dc76e9c31c79fcfb", "start_type": "TTG", "rbs_motif": null, "genes": [ "rplC" ], "truncated": "5-prime", "pseudogene": { "start": 190121, "stop": 190798, "observations": { "mutation": [ 190400 ], "stop": [ 190400 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47397", "query_cov": 1.0, "subject_cov": 0.48638132295719844, "identity": 0.9840000000000001, "score": 233.0, "evalue": 2.05e-75, "gene_start": 1, "gene_end": 125, "reference_start": 133, "reference_end": 257, "reference_sequence": "MDVRGIFGVKVGMSQIFTEQNECLPITIVYCEANQVAGIKTIAKDNYNATLLSFQTVDEKQLNKPKQGFFSKLKLEPHKYLREIRKMQGFELGKKITPQELFKIGEYVDVTSLTKGRGFTGAIKRWNFKIGPLGHGAGYPHRFQGSVQAGRGGSSAQRVFKGKKMSGHYGHEQVTIQNLFIVGFDEINKLVLVSGAIAGPEGGIVLIKTAKKKTGKIKDIKLAVQTVKAPQLKAPKKQKTKVETNQVNPKIEEEKTK" }, "paralog": false, "identity": 0.9911111111111112, "score": 442.0, "evalue": 1.69e-160, "description": "Internal stop codon at 190400. Nonsense mutation around 190400.", "psc": { "uniref90_id": "UniRef90_P47397", "gene": "rplC", "product": "50S ribosomal protein L3", "uniref50_id": "UniRef50_P75580", "cog_id": "COG0087", "cog_category": "J", "kegg_orthology_id": "K02906", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47397", "UniRef:UniRef50_P75580", "COG:COG0087", "COG:J", "KEGG:K02906", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P75580", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75580" ], "product": "Large ribosomal subunit protein uL3" } }, "id": "KMADJIBOEG_382", "locus": "GCA000292445_00346" }, { "type": "cds", "sequence": "contig_1", "start": 190798, "stop": 191433, "strand": "+", "frame": 1, "gene": "rplD", "product": "50S ribosomal protein L4", "db_xrefs": [ "COG:COG0088", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:1990904", "RefSeq:WP_009885836.1", "SO:0001217", "UniParc:UPI00001340F9", "UniRef:UniRef100_P47398", "UniRef:UniRef50_P75579", "UniRef:UniRef90_P47398" ], "nt": "ATGGCTAAGCTTAAAGTAATCCAGTTTGATGGTAGTTTTAAAGGTGAGATCCAACCTGCTAACCACCTCCTTTTAAAAAAAGCAGTGATCCAACCAGTGTTTGATGCTATCTTATTAGAACAAGCAGCATGTAGACAAGGCACTCACTCCACTTTAACTAAGGGTGAAGTTAGTGGTGGGGGTAAAAAACCATATAAACAAAAGCACACTGGTAAAGCTAGACAAGGTTCAATAAGAAACCCCCATTATGTGGGGGGAGGTGTTGTTTTTGGTCCTAAACCCAACCGTAACTACAAACTAAAACTAAACAAAAAGGCTTATCAACTTGCTTTAACTAGTGCCTTTGCACAAAAGCTTAACAACAACCAAGTGATAGTTGCTGAAGCCAAGTTGTTTGAACAAACCAATGCCAAAACTAAAAAGATGCTGACGTTTCTCAAGAATGCCAAACTAACTGAGCAAAAACTCTTGTTTGTGATTGATACTATCTCAAAACCACTGTTGTTGAGTACTAACAACCTAAAGCAGATAGTAGTCAAACAGTTTAATAAAGTATCAGTAAGAGATCTACTTTTAGCTAAAACTATCATCATTGAAAAAGCTGCTTTTACAAAACTGGAGGAACGACTTAAATAG", "aa": "MAKLKVIQFDGSFKGEIQPANHLLLKKAVIQPVFDAILLEQAACRQGTHSTLTKGEVSGGGKKPYKQKHTGKARQGSIRNPHYVGGGVVFGPKPNRNYKLKLNKKAYQLALTSAFAQKLNNNQVIVAEAKLFEQTNAKTKKMLTFLKNAKLTEQKLLFVIDTISKPLLLSTNNLKQIVVKQFNKVSVRDLLLAKTIIIEKAAFTKLEERLK", "aa_hexdigest": "4ef0c6907ae54099008de7d7cf4cd805", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001340F9", "ncbi_nrp_id": "WP_009885836.1", "uniref100_id": "UniRef100_P47398", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001340F9", "RefSeq:WP_009885836.1", "UniRef:UniRef100_P47398" ] }, "ips": { "uniref100_id": "UniRef100_P47398", "uniref90_id": "UniRef90_P47398" }, "psc": { "uniref90_id": "UniRef90_P47398", "gene": "rplD", "product": "50S ribosomal protein L4", "uniref50_id": "UniRef50_P75579", "cog_id": "COG0088", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P75579", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75579" ], "product": "Large ribosomal subunit protein uL4" }, "genes": [ "rplD" ], "id": "KMADJIBOEG_383", "locus": "GCA000292445_00347" }, { "type": "cds", "sequence": "contig_1", "start": 191437, "stop": 191757, "strand": "+", "frame": 1, "gene": "rplW", "product": "50S ribosomal protein L23", "db_xrefs": [ "COG:COG0089", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "RefSeq:WP_009885837.1", "SO:0001217", "UniParc:UPI0000133DA2", "UniRef:UniRef100_P47399", "UniRef:UniRef50_P47399", "UniRef:UniRef90_P47399" ], "nt": "ATGGATGTAACCAACATACTCTTAAAACCAGTCTTAACTGAAAAGAGTTATCTCAACCAGATGGGGGAATTGAAAAAATATGTCTTTGCAATTAACCCTAAAGCTACTAAAACCAAAGTAAAACTAGCGTTTGAAATTATCTATGGGGTTAAACCTTTAAAGATTAACACGCTAATTAGAAAACCAGTGACCATTAGAAATGGCACTAAATACCCTGGGTTTAGTAAGCTAGCAAAACTAGCAGTAATCACCTTACCTAAGGGAATGGATATTGCCATTACTGGTGAGAAAACAACCAAGAAAGAAACAAAGGATCAATAA", "aa": "MDVTNILLKPVLTEKSYLNQMGELKKYVFAINPKATKTKVKLAFEIIYGVKPLKINTLIRKPVTIRNGTKYPGFSKLAKLAVITLPKGMDIAITGEKTTKKETKDQ", "aa_hexdigest": "cb3bde973f635e2c137d7725dd7ff6fe", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133DA2", "ncbi_nrp_id": "WP_009885837.1", "uniref100_id": "UniRef100_P47399", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133DA2", "RefSeq:WP_009885837.1", "UniRef:UniRef100_P47399" ] }, "ips": { "uniref100_id": "UniRef100_P47399", "uniref90_id": "UniRef90_P47399" }, "psc": { "uniref90_id": "UniRef90_P47399", "gene": "rplW", "product": "50S ribosomal protein L23", "uniref50_id": "UniRef50_P47399", "cog_id": "COG0089", "cog_category": "J", "go_ids": [ "GO:1990904", "GO:0005840", "GO:0019843", "GO:0003735", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P47399", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47399" ], "product": "Large ribosomal subunit protein uL23" }, "genes": [ "rplW" ], "id": "KMADJIBOEG_384", "locus": "GCA000292445_00348" }, { "type": "cds", "sequence": "contig_1", "start": 191757, "stop": 192614, "strand": "+", "frame": 3, "gene": "rplB", "product": "50S ribosomal protein L2", "db_xrefs": [ "COG:COG0090", "COG:J", "GO:0002181", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740", "GO:0019843", "GO:0022625", "KEGG:K02886", "RefSeq:WP_009885838.1", "SO:0001217", "UniParc:UPI0000133ECD", "UniRef:UniRef100_P47400", "UniRef:UniRef50_Q9AMK8", "UniRef:UniRef90_P47400" ], "nt": "ATGGCAATTAAAAAGATTATTAGTCGTTCTAACAGTGGGATTCACAACGCCACTGTCATTGACTTTAAAAAACTCCTTACCAATTCCAAACCCGAAAAGTCGCTTTTAGTTACTTTAAAAAAACATGCAGGAAGAAACAACCAGGGCAAGATCACTGTTCGCCACCACGGTGGGAGACATAAACGTAAGTACCGTTTAATTGATTTTAAGCGTTACCACTATGACAATTTAAAAGCAACTGTTAAATCGATTGAATATGATCCTAACCGCAGTTGTTTTATCTCCCTTTTACACTATCAGAATGGGGTTAAAACTTACATCATTAGTCCTGATGGGATTAAGGTTGGTGATCAAGTTTATTCATCTGATCATGCCATTGATATCAAACTAGGTTATTGTATGCCCCTTGCTTTTATCCCTGAAGGAACCCAAGTTCATAACATTGAACTTAACCCTAAGGGTGGGGGTAAGATAGCAAGAAGTGCTGGAAGTTATGCGAGGATCTTGGGTCAAGATGAGACTGGTAAATACATCATTCTCCAGTTAATCTCAGGGGAAACTAGGAAGTTTTTAAAGGAGTGTAGAGCTACAGTTGGTGTTGTCTCTAACTTAGATCATAACCTTGTTGTAATTGGTAAAGCAGGGAGAAGTCGTCATAAGGGAATCAGACCAACGGTTAGAGGTTCAGCAATGAACCCTAATGACCACCCGCATGGGGGTGGGGAAGGGAGAAGCCCAGTTGGCAGAGATGCACCAAGAACCCCTTGGGGCAAACGCCATATGGGTGTGAAAACACGTAACATGAAAAAACATTCAACTAACCTGATTATTAGAAACAGAAAAGGAGAACAATACTAA", "aa": "MAIKKIISRSNSGIHNATVIDFKKLLTNSKPEKSLLVTLKKHAGRNNQGKITVRHHGGRHKRKYRLIDFKRYHYDNLKATVKSIEYDPNRSCFISLLHYQNGVKTYIISPDGIKVGDQVYSSDHAIDIKLGYCMPLAFIPEGTQVHNIELNPKGGGKIARSAGSYARILGQDETGKYIILQLISGETRKFLKECRATVGVVSNLDHNLVVIGKAGRSRHKGIRPTVRGSAMNPNDHPHGGGEGRSPVGRDAPRTPWGKRHMGVKTRNMKKHSTNLIIRNRKGEQY", "aa_hexdigest": "b29c106011d890d3aa3a4e50207ce901", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133ECD", "ncbi_nrp_id": "WP_009885838.1", "uniref100_id": "UniRef100_P47400", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133ECD", "RefSeq:WP_009885838.1", "UniRef:UniRef100_P47400" ] }, "ips": { "uniref100_id": "UniRef100_P47400", "uniref90_id": "UniRef90_P47400" }, "psc": { "uniref90_id": "UniRef90_P47400", "gene": "rplB", "product": "50S ribosomal protein L2", "uniref50_id": "UniRef50_Q9AMK8", "cog_id": "COG0090", "cog_category": "J", "kegg_orthology_id": "K02886", "go_ids": [ "GO:0002181", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q9AMK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9AMK8" ], "product": "Large ribosomal subunit protein uL2" }, "genes": [ "rplB" ], "id": "KMADJIBOEG_385", "locus": "GCA000292445_00349" }, { "type": "cds", "sequence": "contig_1", "start": 192614, "stop": 192877, "strand": "+", "frame": 2, "gene": "rpsS", "product": "30S ribosomal protein S19", "db_xrefs": [ "COG:COG0185", "COG:J", "GO:0000028", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0022627", "RefSeq:WP_009885839.1", "SO:0001217", "UniParc:UPI0000134DEA", "UniRef:UniRef100_P47401", "UniRef:UniRef50_Q6ME59", "UniRef:UniRef90_P75576" ], "nt": "ATGTCAAGAAGTAGTAAAAAGGGCGCATTTGTTGATGCTCACCTCTTAAAAAAAGTGATTGAAATGAACAAACAAGCCAAGAAAAAACCAATTAAGACTTGGTCAAGAAGAAGTACTATCTTCCCTGAGTTTGTGGGTAACACCTTCAGTGTGCATAACGGTAAAACCTTTATTAATGTTTATGTTACTGATGATATGGTAGGTCATAAGTTGGGTGAGTTTTCCCCAACTAGAAACTTTAAACAACACACTGCTAACCGTTAG", "aa": "MSRSSKKGAFVDAHLLKKVIEMNKQAKKKPIKTWSRRSTIFPEFVGNTFSVHNGKTFINVYVTDDMVGHKLGEFSPTRNFKQHTANR", "aa_hexdigest": "3f214f53aa7f11b4874ded3835d887c3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134DEA", "ncbi_nrp_id": "WP_009885839.1", "uniref100_id": "UniRef100_P47401", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134DEA", "RefSeq:WP_009885839.1", "UniRef:UniRef100_P47401" ] }, "ips": { "uniref100_id": "UniRef100_P47401", "uniref90_id": "UniRef90_P75576" }, "psc": { "uniref90_id": "UniRef90_P75576", "gene": "rpsS", "product": "30S ribosomal protein S19", "uniref50_id": "UniRef50_Q6ME59", "cog_id": "COG0185", "cog_category": "J", "go_ids": [ "GO:0000028", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_Q6ME59", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6ME59" ], "product": "Small ribosomal subunit protein uS19" }, "genes": [ "rpsS" ], "id": "KMADJIBOEG_386", "locus": "GCA000292445_00350" }, { "type": "cds", "sequence": "contig_1", "start": 192880, "stop": 193314, "strand": "+", "frame": 1, "gene": "rplV", "product": "50S ribosomal protein L22", "db_xrefs": [ "COG:COG0091", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625", "KEGG:K02890", "RefSeq:WP_009885840.1", "SO:0001217", "UniParc:UPI0000133D78", "UniRef:UniRef100_P47402", "UniRef:UniRef50_P47402", "UniRef:UniRef90_P47402" ], "nt": "ATGATTGCTTTTGCTAAACAATACAGAGTTCACATCTCCCCCCAAAAAGCACGTTTAGTGTGCCAGTTAATTGTGGGTAAAAAGATTAATGATGCGCAAAACATCCTTTTAAATACGCCAAAGAAAGCTGCTTACTTTTTAACTAAGTTACTAAATAGTGCGATTAGTAATGCCACTAATAACCACGGGATGAGCGGGGATCTTTTGTATGTATTTGAATGTGTTGCTAACCAAGGACCTAGCATGAAAAGAACAATCGCTAGAGCCAAAGGTTCAGGGAGTGTTTTAACCAAGCGTTCTTCAAACCTAGTTATTAAGTTATCTGATAATCCCAATGAAAGAAAATTACTCTTAACCCAACAAAAGGAACTGGTGAAAAAAAGAACAATGGGTCATAAAAAAGAGAAAGCAAAGCAAAAGCAAAAACAACAATAA", "aa": "MIAFAKQYRVHISPQKARLVCQLIVGKKINDAQNILLNTPKKAAYFLTKLLNSAISNATNNHGMSGDLLYVFECVANQGPSMKRTIARAKGSGSVLTKRSSNLVIKLSDNPNERKLLLTQQKELVKKRTMGHKKEKAKQKQKQQ", "aa_hexdigest": "5ef95273909311097b2a84d0940b84a8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133D78", "ncbi_nrp_id": "WP_009885840.1", "uniref100_id": "UniRef100_P47402", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133D78", "RefSeq:WP_009885840.1", "UniRef:UniRef100_P47402" ] }, "ips": { "uniref100_id": "UniRef100_P47402", "uniref90_id": "UniRef90_P47402" }, "psc": { "uniref90_id": "UniRef90_P47402", "gene": "rplV", "product": "50S ribosomal protein L22", "uniref50_id": "UniRef50_P47402", "cog_id": "COG0091", "cog_category": "J", "kegg_orthology_id": "K02890", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47402", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47402" ], "product": "Large ribosomal subunit protein uL22" }, "genes": [ "rplV" ], "id": "KMADJIBOEG_387", "locus": "GCA000292445_00351" }, { "type": "cds", "sequence": "contig_1", "start": 193473, "stop": 194123, "strand": "+", "frame": 3, "gene": "rpsC", "product": "30S ribosomal protein S3", "db_xrefs": [ "COG:COG0092", "COG:J", "GO:0003729", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022627", "KEGG:K02982", "SO:0001217", "UniRef:UniRef50_O52338", "UniRef:UniRef90_P47403" ], "nt": "GTGTCTAATGTTGAGATAGAAAGAACCCAAACGACTGTTGATGTTTATGTCTATGCAGCTCAACCTGCTTTATTGATAGGCAGTGAAAACAAAAACATCCAAAAGATTACCAAAATGATCCAAATCATTGTGGGCAGAAAGATTAAACTTGATCTTACTATCAATGAGATCGGCTCTCCGATGTTATCAAGTAGGATCATTGCCCGTGATATTGCTAATGCGATTGAAAACAGAGTACCACTCCGTTCAGCAATGCGCCAAGCTCTAACCAAGGTTTTAAAAGCAGGTGCTAATGGGATTAAGGTATTGGTATCAGGCAGATTAAATGGGGCGGAAATTGCCCGTGACAAGATGTATATTGAGGGCAATATGCCTCTTTCAACTTTAAGAGCAGATATTGACTATGCCTTTGAAAAAGCAAAAACCACCTATGGCATTATTGGGGTGAAAGTATGGATTAACAGGGGGATGATCTATGCAAAGGGTTTAAACAGAACCCCAGCACACATCCTCCATCCCCAAAAGAAACAGCTAAAAACCCCAACTATCAAAAAAACCAATTCAGTAATAGCAAAACAAAAACTCACTGGTAGTGATATTGAAACTGCTAGTTTAAAAGCACTTACTGATAATAATCAAAACCACGAATAG", "aa": "MSNVEIERTQTTVDVYVYAAQPALLIGSENKNIQKITKMIQIIVGRKIKLDLTINEIGSPMLSSRIIARDIANAIENRVPLRSAMRQALTKVLKAGANGIKVLVSGRLNGAEIARDKMYIEGNMPLSTLRADIDYAFEKAKTTYGIIGVKVWINRGMIYAKGLNRTPAHILHPQKKQLKTPTIKKTNSVIAKQKLTGSDIETASLKALTDNNQNHE", "aa_hexdigest": "44fb446fc3d65b5806df197fd2afc470", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47403", "query_cov": 1.0, "subject_cov": 0.8059701492537313, "identity": 0.995, "score": 367.0, "evalue": 1.36e-126, "valid": true, "gene": "rpsC", "product": "30S ribosomal protein S3", "uniref50_id": "UniRef50_O52338", "cog_id": "COG0092", "cog_category": "J", "kegg_orthology_id": "K02982", "go_ids": [ "GO:0003729", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_O52338", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52338" ], "product": "Small ribosomal subunit protein uS3" }, "genes": [ "rpsC" ], "id": "KMADJIBOEG_388", "locus": "GCA000292445_00352" }, { "type": "cds", "sequence": "contig_1", "start": 194129, "stop": 194323, "strand": "+", "frame": 2, "gene": "rplP", "product": "50S ribosomal protein L16", "db_xrefs": [ "COG:COG0197", "COG:J", "GO:0000049", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022625", "KEGG:K02878", "SO:0001217", "UniRef:UniRef50_P02415", "UniRef:UniRef90_P47404" ], "nt": "ATGTTACAACCAAAAAGAACCAAATACAGAAAACCACATAACGTCAGTTATGAAGGACACACTAAGGGCAATGGTTATGTTGCTTTTGGTGAGTATGGAATTGTTGCTACTAAGGGTAATTGGATCGATGCGAGAGCAATTGAATCAGCGCGGGTTGCTATCTCAAAGTGCTTGGGTAAAACTGGAAAGATGTGA", "aa": "MLQPKRTKYRKPHNVSYEGHTKGNGYVAFGEYGIVATKGNWIDARAIESARVAISKCLGKTGKM", "aa_hexdigest": "b9d8b1d6fece06001424e9d7004b3a9a", "start_type": "ATG", "rbs_motif": null, "genes": [ "rplP" ], "truncated": "3-prime", "pseudogene": { "start": 194129, "stop": 194545, "observations": { "mutation": [ 194321, 194513 ], "stop": [ 194321, 194513 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47404", "query_cov": 1.0, "subject_cov": 0.460431654676259, "identity": 0.922, "score": 124.0, "evalue": 4.4e-35, "gene_start": 1, "gene_end": 64, "reference_start": 1, "reference_end": 64, "reference_sequence": "MLQPKRTKYRKPHNVSYEGKAKGNSYVAFGEYGLVATKGNWIDARAIESARIAISKCLGKTGKMWIRIFPHMSKTKKPLEVRMGSGKGNPEFWVAVVKQGTVMFEVANIPESQMIKALTRAGHKLPVTWKILKREEVSA" }, "paralog": false, "identity": 0.9130434782608695, "score": 258.0, "evalue": 1.42e-90, "description": "Internal stop codon at 194321, 194513. Nonsense mutation around 194321, 194513.", "psc": { "uniref90_id": "UniRef90_P47404", "gene": "rplP", "product": "50S ribosomal protein L16", "uniref50_id": "UniRef50_P02415", "cog_id": "COG0197", "cog_category": "J", "kegg_orthology_id": "K02878", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022625" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47404", "UniRef:UniRef50_P02415", "COG:COG0197", "COG:J", "KEGG:K02878", "GO:0000049", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P02415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P02415" ], "product": "Large ribosomal subunit protein uL16" } }, "id": "KMADJIBOEG_389", "locus": "GCA000292445_00353" }, { "type": "cds", "sequence": "contig_1", "start": 194342, "stop": 194515, "strand": "+", "frame": 2, "gene": null, "product": "Large ribosomal subunit protein uL16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_M5SUM2" ], "nt": "ATGTCAAAAACCAAAAAACCCTTAGAAGTGAGGATGGGTTCAGGGAAAGGTAACCCTGAATTTTGGGTTGCTGTTGTTAAAAAGGGGACAGTGATGTTTGAAGTTGCTAACATCCCTGAACAACAGATGATCAAAGCCTTAACAAGAGCAGGCCATAAACTCCCTGTTACCTGA", "aa": "MSKTKKPLEVRMGSGKGNPEFWVAVVKKGTVMFEVANIPEQQMIKALTRAGHKLPVT", "aa_hexdigest": "40ea8c93f2f0ea231b9d0e2882bcb9b2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_M5SUM2", "query_cov": 0.9298245614035088, "subject_cov": 0.828125, "identity": 0.509, "score": 63.9, "evalue": 2.35e-12, "valid": false, "product": "Large ribosomal subunit protein uL16", "uniref50_id": "UniRef50_M5SUM2" }, "pscc": { "uniref50_id": "UniRef50_M5SUM2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_M5SUM2" ], "product": "Large ribosomal subunit protein uL16" }, "genes": [], "id": "KMADJIBOEG_390", "locus": "GCA000292445_00354" }, { "type": "cds", "sequence": "contig_1", "start": 194545, "stop": 194739, "strand": "+", "frame": 1, "gene": "rpmC", "product": "50S ribosomal protein L29", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "SO:0001217", "UniRef:UniRef50_UPI0029620CC7", "UniRef:UniRef90_UPI0029620CC7" ], "nt": "ATGACAATCGCTAAGGAGCTGAAGCAAAAGAGCAACGAAGAGTTAGTGAAACTAGTAATTAAGCTTAAGGGTGAACTCTTAGAATACCGCTTTAAACTTGCCCATGGTGAACTTGACAAACCCCATCTGATTGCCAAGGTGAGAAAGTTATTAGCAGTTGTACTTACTATTCTCACTGAACGCAAACTCAACTGA", "aa": "MTIAKELKQKSNEELVKLVIKLKGELLEYRFKLAHGELDKPHLIAKVRKLLAVVLTILTERKLN", "aa_hexdigest": "2b3b8b6dc7f453cfcd94f3666e7c3513", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpmC" ], "truncated": "3-prime", "pseudogene": { "start": 194551, "stop": 194859, "observations": { "mutation": [ 194737, 194803 ], "stop": [ 194737, 194803 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0029620CC7", "query_cov": 0.96875, "subject_cov": 0.4460431654676259, "identity": 0.968, "score": 111.0, "evalue": 3.23e-30, "gene_start": 3, "gene_end": 64, "reference_start": 1, "reference_end": 62, "reference_sequence": "MAKELKQKSNEELVKLVIKLKGELLEYRFKLAHGELDKPHLIAKVRKLLAVVLTILTERKINWQVEKDKYKLLSRKTNELIVNSWKQKLSTKPESKQETKKAEVKPKVEPKPLKQETKKVEENKKLKRLKLNLKLNQNL" }, "paralog": false, "identity": 0.9607843137254902, "score": 185.0, "evalue": 6.63e-62, "description": "Internal stop codon at 194737, 194803. Nonsense mutation around 194737, 194803.", "psc": { "uniref90_id": "UniRef90_UPI0029620CC7", "gene": "rpmC", "product": "50S ribosomal protein L29", "uniref50_id": "UniRef50_UPI0029620CC7", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0029620CC7", "UniRef:UniRef50_UPI0029620CC7", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0029620CC7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0029620CC7" ], "product": "50S ribosomal protein L29" } }, "id": "KMADJIBOEG_391", "locus": "GCA000292445_00355" }, { "type": "cds", "sequence": "contig_1", "start": 195147, "stop": 195371, "strand": "+", "frame": 3, "gene": "rpsQ", "product": "30S ribosomal protein S17", "db_xrefs": [ "COG:COG0186", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "SO:0001217", "UniRef:UniRef50_P47406", "UniRef:UniRef90_P47406" ], "nt": "ATGAAGCGCAACCAACGTAAGCAGTTAATTGGCACAGTTGTTAGCACCAAAAATGCTAAAACAGCAACTGTCAAAGTAACATCACGCTTTAAACATCCTTTGTATCACAAATCAGTTATTCGCCATAAAAAGTACCATGTCCATAACTTTGGTGAACTTGTTGCTAATGATGGTGATAGGGTACAAATTATTGAAACAAGACCCCTTTCCGCTTTAAAGCGGTGA", "aa": "MKRNQRKQLIGTVVSTKNAKTATVKVTSRFKHPLYHKSVIRHKKYHVHNFGELVANDGDRVQIIETRPLSALKR", "aa_hexdigest": "0ccbd0b07373a87d1ad21f01e9db1014", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47406", "query_cov": 1.0, "subject_cov": 0.8705882352941177, "identity": 1.0, "score": 144.0, "evalue": 7.83e-44, "valid": true, "gene": "rpsQ", "product": "30S ribosomal protein S17", "uniref50_id": "UniRef50_P47406", "cog_id": "COG0186", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P47406", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47406" ], "product": "Small ribosomal subunit protein uS17" }, "genes": [ "rpsQ" ], "id": "KMADJIBOEG_392", "locus": "GCA000292445_00356" }, { "type": "cds", "sequence": "contig_1", "start": 195408, "stop": 195776, "strand": "+", "frame": 3, "gene": "rplN", "product": "50S ribosomal protein L14", "db_xrefs": [ "COG:COG0093", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0022625", "GO:0070180", "KEGG:K02874", "RefSeq:WP_009885845.1", "SO:0001217", "UniParc:UPI0000133BE7", "UniRef:UniRef100_P47407", "UniRef:UniRef50_Q5SHP8", "UniRef:UniRef90_P47407" ], "nt": "ATGGTTAGTTTTATGACAAGATTAAATGTAGCTGATAATACAGGCGCTAAGCAAGTAGGTATTATCAAAGTTTTAGGTGCTACATACAAACGTTATGCATTCCTTGGTGATGTTGTTGTTGTATCAGTTAAAGATGCAATCCCTAATGGCATGGTTAAAAAGGGTCAAGTGTTAAGAGCAGTCATTGTTAGAACCAAAAAGGGACAACAACGCCAAGATGGTACCCACCTAAAGTTCCATGACAATGCTTGTGTGCTTATCAAAGAAGATAAATCCCCAAGGGGAACAAGAATCTTTGGGCCAGTTGCTAGGGAGTTGAGAGAAAAAGGTTACAACAAGATTTTAAGCTTGGCGGTGGAGGTTGTTTAA", "aa": "MVSFMTRLNVADNTGAKQVGIIKVLGATYKRYAFLGDVVVVSVKDAIPNGMVKKGQVLRAVIVRTKKGQQRQDGTHLKFHDNACVLIKEDKSPRGTRIFGPVARELREKGYNKILSLAVEVV", "aa_hexdigest": "a3dba71f87df18733f7f3013a4c99584", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133BE7", "ncbi_nrp_id": "WP_009885845.1", "uniref100_id": "UniRef100_P47407", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133BE7", "RefSeq:WP_009885845.1", "UniRef:UniRef100_P47407" ] }, "ips": { "uniref100_id": "UniRef100_P47407", "uniref90_id": "UniRef90_P47407" }, "psc": { "uniref90_id": "UniRef90_P47407", "gene": "rplN", "product": "50S ribosomal protein L14", "uniref50_id": "UniRef50_Q5SHP8", "cog_id": "COG0093", "cog_category": "J", "kegg_orthology_id": "K02874", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0022625", "GO:0070180" ] }, "pscc": { "uniref50_id": "UniRef50_Q5SHP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5SHP8" ], "product": "Large ribosomal subunit protein uL14" }, "genes": [ "rplN" ], "id": "KMADJIBOEG_393", "locus": "GCA000292445_00357" }, { "type": "cds", "sequence": "contig_1", "start": 195776, "stop": 196102, "strand": "+", "frame": 2, "gene": "rplX", "product": "50S ribosomal protein L24", "db_xrefs": [ "COG:COG0198", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "RefSeq:WP_014894185.1", "SO:0001217", "UniParc:UPI00027B3997", "UniRef:UniRef100_UPI00027B3997", "UniRef:UniRef50_O52343", "UniRef:UniRef90_P47408" ], "nt": "ATGCAAAGGATTAGAAAAGGTGATAAGGTAGTTGTGATCACTGGTAAAAACAAGGGCGGTAGTGGGATAGTGCTTAAGGTATTAACCAAGCAAAACAAAGCGATTGTTGAGGGGATCAATAAGGTTACTGTTCACAAAAAAGAACAAGTCAACAAGCACAACAAACAAACAAACCCAACTACTAAAGAAGCCCCTTTACCATTAAATAAACTTGCTTTATTTGATCAGAAGGCCAAACAGCAAACAATTGGCAAGATCAAATACCAAATTGATCCTAAAACCAAACAAAAAACAAGAGTCTTTAAAAAGACTAATAATGCCATTTAA", "aa": "MQRIRKGDKVVVITGKNKGGSGIVLKVLTKQNKAIVEGINKVTVHKKEQVNKHNKQTNPTTKEAPLPLNKLALFDQKAKQQTIGKIKYQIDPKTKQKTRVFKKTNNAI", "aa_hexdigest": "03990510080fd5814e4d181a437bfe12", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3997", "ncbi_nrp_id": "WP_014894185.1", "uniref100_id": "UniRef100_UPI00027B3997", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3997", "RefSeq:WP_014894185.1", "UniRef:UniRef100_UPI00027B3997" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3997", "uniref90_id": "UniRef90_P47408" }, "psc": { "uniref90_id": "UniRef90_P47408", "gene": "rplX", "product": "50S ribosomal protein L24", "uniref50_id": "UniRef50_O52343", "cog_id": "COG0198", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_O52343", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52343" ], "product": "Large ribosomal subunit protein uL24" }, "genes": [ "rplX" ], "id": "KMADJIBOEG_394", "locus": "GCA000292445_00358" }, { "type": "cds", "sequence": "contig_1", "start": 196108, "stop": 196398, "strand": "+", "frame": 1, "gene": null, "product": "50S ribosomal protein L5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380ENH6" ], "nt": "ATGAATAACCTTGAAAAAACCTATAAAACTGCGTTAGTTAATCAACTCCAACAACAGTTGGGCTTTTCTTCCATCATGCAAGTCCCTAAGTTAACAAAAATCGTTGTTAACATGGGAGTTGGGGATGCAATTAGAGACAACAAGTTCCTTGAATCAGCACTAAATGAACTGCACCTGATTACTGGTCAAAAACCTGTTGCTACTAAAGCTAAGAATGCTATCTCAACTTACAAGTTACGTGCTGGTCAATTAATTGGTTGTAAAGTTACTCTAAGAAATAAAAAGATGTGA", "aa": "MNNLEKTYKTALVNQLQQQLGFSSIMQVPKLTKIVVNMGVGDAIRDNKFLESALNELHLITGQKPVATKAKNAISTYKLRAGQLIGCKVTLRNKKM", "aa_hexdigest": "2f01ed4f710456a260d3558900ce2dd1", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A380ENH6", "query_cov": 0.9791666666666666, "subject_cov": 0.9494949494949495, "identity": 0.532, "score": 107.0, "evalue": 1.77e-28, "valid": false, "product": "50S ribosomal protein L5", "uniref50_id": "UniRef50_A0A380ENH6", "kegg_orthology_id": "K02931" }, "pscc": { "uniref50_id": "UniRef50_A0A380ENH6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380ENH6" ], "product": "50S ribosomal protein L5" }, "genes": [], "id": "KMADJIBOEG_395", "locus": "GCA000292445_00359" }, { "type": "cds", "sequence": "contig_1", "start": 196653, "stop": 196835, "strand": "+", "frame": 3, "gene": "rpsZ", "product": "type Z 30S ribosomal protein S14", "db_xrefs": [ "COG:COG0199", "COG:J", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0008270", "GO:0015935", "GO:0019843", "KEGG:K02954", "SO:0001217", "UniRef:UniRef50_P12878", "UniRef:UniRef90_P47410" ], "nt": "ATGGCTAAAAAATCATTAAAAGTAAAACAATCCCGTCCCAATAAGTTTAGTGTACGCGACTACACCAGGTGTTTAAGGTGTGGGCGTGCTAGAGCAGTGTTAAGCCACTTTGGTGTGTGTAGGTTGTGTTTCCGTGAACTTGCTTATGCAGGAGCAATCCCAGGAGTTAAAAAAGCATCATGA", "aa": "MAKKSLKVKQSRPNKFSVRDYTRCLRCGRARAVLSHFGVCRLCFRELAYAGAIPGVKKAS", "aa_hexdigest": "985e1a9c2861017fe85276491b751686", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47410", "query_cov": 1.0, "subject_cov": 0.9836065573770492, "identity": 1.0, "score": 120.0, "evalue": 6.41e-35, "valid": true, "gene": "rpsZ", "product": "type Z 30S ribosomal protein S14", "uniref50_id": "UniRef50_P12878", "cog_id": "COG0199", "cog_category": "J", "kegg_orthology_id": "K02954", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0008270", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_P12878", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P12878" ], "product": "Small ribosomal subunit protein uS14B" }, "genes": [ "rpsZ" ], "id": "KMADJIBOEG_396", "locus": "GCA000292445_00360" }, { "type": "cds", "sequence": "contig_1", "start": 196832, "stop": 197257, "strand": "+", "frame": 2, "gene": "rpsH", "product": "30S ribosomal protein S8", "db_xrefs": [ "COG:COG0096", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "RefSeq:WP_010869359.1", "SO:0001217", "UniParc:UPI000013516D", "UniRef:UniRef100_P47411", "UniRef:UniRef50_Q9PQP6", "UniRef:UniRef90_P47411" ], "nt": "ATGATAATCAATAAAGTTCCCAAAGCCCATTTTGATCCAGTTTCTGATCTTTTCACTAAGATCAACAATGCTAGAAAAGCTAAGCTTTTAACTGTTACCACCATCGCTTCTAAGTTAAAGATAGCTATCTTAGAGATTTTGATTAAAGAGGGCTATTTAGCTAACTATCAGGTGTTGGAAAATAAAACTAAAACCAAAAAACTAGTTAGTTTCACATTAAAATACACCCAAAGAAGGATATGTTCTATTAATGGGGTGAAACAGATCTCAAAACCAGGATTAAGAATCTATCGTTCCTTTGAAAAACTTCCCCTTGTTTTAAATGGTCTTGGTATTGCAATTATCTCCACTAGTGATGGAGTGATGACTGATAAAGTAGCAAGGTTAAAGAAGATTGGTGGGGAGATTTTAGCTTACGTTTGGTAA", "aa": "MIINKVPKAHFDPVSDLFTKINNARKAKLLTVTTIASKLKIAILEILIKEGYLANYQVLENKTKTKKLVSFTLKYTQRRICSINGVKQISKPGLRIYRSFEKLPLVLNGLGIAIISTSDGVMTDKVARLKKIGGEILAYVW", "aa_hexdigest": "d93016cb3156fd323ac12380b7f7223e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013516D", "ncbi_nrp_id": "WP_010869359.1", "uniref100_id": "UniRef100_P47411", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013516D", "RefSeq:WP_010869359.1", "UniRef:UniRef100_P47411" ] }, "ips": { "uniref100_id": "UniRef100_P47411", "uniref90_id": "UniRef90_P47411" }, "psc": { "uniref90_id": "UniRef90_P47411", "gene": "rpsH", "product": "30S ribosomal protein S8", "uniref50_id": "UniRef50_Q9PQP6", "cog_id": "COG0096", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP6" ], "product": "Small ribosomal subunit protein uS8" }, "genes": [ "rpsH" ], "id": "KMADJIBOEG_397", "locus": "GCA000292445_00361" }, { "type": "cds", "sequence": "contig_1", "start": 197264, "stop": 197725, "strand": "+", "frame": 2, "gene": "rplF", "product": "50S ribosomal protein L6", "db_xrefs": [ "COG:COG0097", "COG:J", "GO:0002181", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "SO:0001217", "UniRef:UniRef50_P47412", "UniRef:UniRef90_P47412" ], "nt": "ATGTCAAAAATAGGAAATAGACCAATCAAAATTGATCCTAGTAAAGTGAGTTTAATGCAAACAACAACACTGCTTACTATTAAAGGACCATTAGGGGAAAACACCATTAAACTACCCAAAAACTTACCCTTAAAGTTTGTTGTTGAAAATGACACTATTAAAGTAACTAATAACAACAACTTAAAACAAACTAAGATCTTACACGGTACTTTCAATGCGTTAGTTAACAACGCAGTTATTGGGGTTACCAAGGGTTTTGAAAAGAAACTCATCCTAGTTGGGGTTGGTTATCGTGCTAATGTGGAAGGGCAATTTCTCAACTTACAGTTGGGCTATTCCCATCCTATTAAGGAGTTGATCCCAAACCAACTTACTGTTAAAGTAGAGAAGAACACTGAAATCACTATTAGTGGAATAAAAAAAGAGTTAGTAGGTCAGTTTGCCACTGAAATCAGAAAGTGA", "aa": "MSKIGNRPIKIDPSKVSLMQTTTLLTIKGPLGENTIKLPKNLPLKFVVENDTIKVTNNNNLKQTKILHGTFNALVNNAVIGVTKGFEKKLILVGVGYRANVEGQFLNLQLGYSHPIKELIPNQLTVKVEKNTEITISGIKKELVGQFATEIRK", "aa_hexdigest": "562434f09928b21fc6e22a740fd5f3e7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47412", "query_cov": 1.0, "subject_cov": 0.8315217391304348, "identity": 0.993, "score": 295.0, "evalue": 1.23e-100, "valid": true, "gene": "rplF", "product": "50S ribosomal protein L6", "uniref50_id": "UniRef50_P47412", "cog_id": "COG0097", "cog_category": "J", "go_ids": [ "GO:0002181", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47412", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47412" ], "product": "Large ribosomal subunit protein uL6" }, "genes": [ "rplF" ], "id": "KMADJIBOEG_398", "locus": "GCA000292445_00362" }, { "type": "cds", "sequence": "contig_1", "start": 197778, "stop": 198167, "strand": "+", "frame": 3, "gene": "rplR", "product": "50S ribosomal protein L18", "db_xrefs": [ "COG:COG0256", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0008097", "GO:0022625", "SO:0001217", "UniRef:UniRef50_P47413", "UniRef:UniRef90_P47413" ], "nt": "TTGTTAGAAAACAAGGTAAAACTGCAGAGGGCAAGAAATAAGATGACAAGAAACGATAAAAGAAGGATTAGACACAAACGGATTGTCAAAAAAATTAGGTTAACTAACCTTAACAACAGGGTTGTACTAATTGTTATTAAGAGTTTAAAAAACATCTCGGTTCAAGCTTGGGACTTTAGTAAGAACGTTGTTTTAACATCAAGTTCCTCACTTCAACTAAAATTAAAAAATGGCAACAAGGAGAATGCTAAACTAGTGGGAATGGATATTGCAACCAAACTCATCAAACTAAACCACAAGGATGTGGTTTTTGATACTGGGGGTAGTAAGTACCATGGTAGGATTGCTGCTTTAGCAGAAGGAGCGCGAGCTAAGGGTTTAAATTTTTAA", "aa": "MLENKVKLQRARNKMTRNDKRRIRHKRIVKKIRLTNLNNRVVLIVIKSLKNISVQAWDFSKNVVLTSSSSLQLKLKNGNKENAKLVGMDIATKLIKLNHKDVVFDTGGSKYHGRIAALAEGARAKGLNF", "aa_hexdigest": "b93699d1af0af835cb51de6d7bbbb88c", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47413", "query_cov": 0.8914728682170543, "subject_cov": 1.0, "identity": 0.991, "score": 211.0, "evalue": 1.11e-68, "valid": true, "gene": "rplR", "product": "50S ribosomal protein L18", "uniref50_id": "UniRef50_P47413", "cog_id": "COG0256", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0008097", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47413", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47413" ], "product": "Large ribosomal subunit protein uL18" }, "genes": [ "rplR" ], "id": "KMADJIBOEG_399", "locus": "GCA000292445_00363" }, { "type": "cds", "sequence": "contig_1", "start": 198172, "stop": 198807, "strand": "+", "frame": 1, "gene": "rpsE", "product": "30S ribosomal protein S5", "db_xrefs": [ "COG:COG0098", "COG:J", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0022627", "KEGG:K02988", "RefSeq:WP_014894188.1", "SO:0001217", "UniParc:UPI00027B399A", "UniRef:UniRef100_UPI00027B399A", "UniRef:UniRef50_Q50301", "UniRef:UniRef90_P47414" ], "nt": "ATGAATGATCCAAAAACTACTAACACTGGCTTGTTAACTTCCACTCTTAAAACCAAGCCCAAACACAACCTTAAACCTTCCAGTGAAGCCATTAAAAAAGCAGTGTCCAAAAAGGAAGGTCACTACAAAAACAAGCGCTTTCAAAAACATAACTTTAATAACAAAAGTGAGTTTGAAGAGAGGATTGTCAAACTTAAACGGATCTCCAAAACCACAAAAGGCGGAAGAAACATGCGCTTTAGTGTCCTTGTTGTTGTTGGTAACAAAAAGGGCAAGGTTGGTTATGGGATTGCTAAGGCATTGGAAGTACCACTTGCCATTAAAAAAGCGATTAAAAAAGCCCATAACTCCATCCATACAGTAGAGATCCACAAGGGCTCGATCTACCACGAAGTGATTGGTAGAAAAGGTGCATCCAAGGTGTTGTTAAAACCTGCACCTTTAGGAACTGGGATCATTGCTGGGGGAGCGATCCGTGCAATTGTAGAGTTAGCTGGTTTTAGTGATATCTATACCAAGAACTTGGGAAGAAACACCCCCATTAACATGATCCATGCCACTATGGATGGGATCTTAAAGCAACTCTCACCCAAAAAAGTGGCATTATTAAGAAATAAACCAATTAGTGATCTATAA", "aa": "MNDPKTTNTGLLTSTLKTKPKHNLKPSSEAIKKAVSKKEGHYKNKRFQKHNFNNKSEFEERIVKLKRISKTTKGGRNMRFSVLVVVGNKKGKVGYGIAKALEVPLAIKKAIKKAHNSIHTVEIHKGSIYHEVIGRKGASKVLLKPAPLGTGIIAGGAIRAIVELAGFSDIYTKNLGRNTPINMIHATMDGILKQLSPKKVALLRNKPISDL", "aa_hexdigest": "70eea6405ca27ee322b06e56fb892f97", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B399A", "ncbi_nrp_id": "WP_014894188.1", "uniref100_id": "UniRef100_UPI00027B399A", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B399A", "RefSeq:WP_014894188.1", "UniRef:UniRef100_UPI00027B399A" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B399A", "uniref90_id": "UniRef90_P47414" }, "psc": { "uniref90_id": "UniRef90_P47414", "gene": "rpsE", "product": "30S ribosomal protein S5", "uniref50_id": "UniRef50_Q50301", "cog_id": "COG0098", "cog_category": "J", "kegg_orthology_id": "K02988", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_Q50301", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50301" ], "product": "Small ribosomal subunit protein uS5" }, "genes": [ "rpsE" ], "id": "KMADJIBOEG_400", "locus": "GCA000292445_00364" }, { "type": "cds", "sequence": "contig_1", "start": 198813, "stop": 199265, "strand": "+", "frame": 3, "gene": "rplO", "product": "50S ribosomal protein L15", "db_xrefs": [ "COG:COG0200", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625", "KEGG:K02876", "RefSeq:WP_010869361.1", "SO:0001217", "UniParc:UPI0000133C15", "UniRef:UniRef100_P47415", "UniRef:UniRef50_P47415", "UniRef:UniRef90_P47415" ], "nt": "ATGGAACTACACCAATTAAAAAGTGTCTCTAAAAGCCGTAACCACAAGTCCAAAGTGGTAGGTAGGGGCCATGGCTCGGGATTAGGTAAAACATCATCACGTGGTCAAAAGGGACAAAAAGCAAGAAAATCAGGTTTAACTAGGTTAGGTTTTGAAGGGGGACAAACACCCCTTTACCGCCGGTTACCTAAGTATGGGGTTGCTAACAAGGGGATCTTAAAAAAAAGGTGGGTTGTTTTAAATTTGAACAAAGTTGCTAAACTCAATCTCAAAACAGTTACTAGAGCAACTTTGATTGAAAAAAAGGTAATTAGTAAAAAAAATAACCTCCCTTTAAAGTTAATTGGGAACACAAAACTCACTACTCCCATCCACTTTGAAGTGCAAAAAATCTCCAAAAATGCTTTAAATGCAGTGCAAACTAGCAAAGGTAGTGTGAAAATTATCACCTAA", "aa": "MELHQLKSVSKSRNHKSKVVGRGHGSGLGKTSSRGQKGQKARKSGLTRLGFEGGQTPLYRRLPKYGVANKGILKKRWVVLNLNKVAKLNLKTVTRATLIEKKVISKKNNLPLKLIGNTKLTTPIHFEVQKISKNALNAVQTSKGSVKIIT", "aa_hexdigest": "4c3312aadfabef538e5148365a549821", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133C15", "ncbi_nrp_id": "WP_010869361.1", "uniref100_id": "UniRef100_P47415", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133C15", "RefSeq:WP_010869361.1", "UniRef:UniRef100_P47415" ] }, "ips": { "uniref100_id": "UniRef100_P47415", "uniref90_id": "UniRef90_P47415" }, "psc": { "uniref90_id": "UniRef90_P47415", "gene": "rplO", "product": "50S ribosomal protein L15", "uniref50_id": "UniRef50_P47415", "cog_id": "COG0200", "cog_category": "J", "kegg_orthology_id": "K02876", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47415" ], "product": "Large ribosomal subunit protein uL15" }, "genes": [ "rplO" ], "id": "KMADJIBOEG_401", "locus": "GCA000292445_00365" }, { "type": "cds", "sequence": "contig_1", "start": 199265, "stop": 200320, "strand": "+", "frame": 2, "gene": "secY", "product": "preprotein translocase subunit SecY", "db_xrefs": [ "COG:COG0201", "COG:U", "GO:0005048", "GO:0005886", "GO:0006616", "GO:0008320", "GO:0015031", "GO:0016020", "GO:0031522", "GO:0043952", "SO:0001217", "UniRef:UniRef50_O52351", "UniRef:UniRef90_P47416" ], "nt": "ATGCAAACTGTTTCTTCACCCAAACAAAAACTTAACTTTGGTCAAAGGTTACTAACTCTATTACAAAACCGTGACTTTATGGTGTCACTGGTTTTAACAGTGGTACTTTTAATCTTGTTTAGGGTGTTAGCAATTATCCCCTTACCAGGGATTAGGATTAATGAGAGTGTCTTGGATAGAAATTCCAATGACTTTTTCTCCCTTTTTAACTTACTTGGGGGTGGGGGATTAAACCAGTTATCGTTGTTTGCAGTTGGGATCAGTCCTTATATCTCAGCCCAAATCATCATGCAACTGCTTTCAACTGATCTAATTCCTCCACTTTCAAAACTAGTTAACAGTGGGGAAGTGGGGCGAAGAAAGATTGAGATGATCACAAGAATTATCACCTTACCCTTTGCTTTAGTGCAAGCATTTGCTGTGATCCAAATTGCTACTAATGCAAGCACTGGTTCAAGTCCGATTAGTTTAGCTAATAGTGGCAGTGAGTTTATTGCTTTTTATATTATTACTATGACTGCAGGGACTTATATGGCAGTGTTTTTGGGTGATACTATCTCCAAAAAGGGGGTTGGTAATGGGATTACTCTGTTAATTCTTTCAGGGATTTTATCCCAACTCCCCCAGGGCTTTATTGCTGCTTACAATGTTTTGAGTGGGATAGTAATTACTTTAACCCCACAGTTAACTGCAGCAATTAGCTTCTTTATCTATTTCTTAGCATTCTTAGTTTTACTGTTTGCCACTACCTTTATCACCCAAGCGACCAGAAAGATTCCCATCCAACAATCAGGACAAGGGTTGGTTAGTGAAGTCAAAACCTTACCTTATTTGCCTATTAAGGTGAATGCTGCTGGGGTGATCCCTGTCATCTTTGCATCCAGTATTATGTCTATCCCTGTGACCATTGCCCAGTTTCAACCCCAAACTGAGTCACGGTGGTTTGTGGAGGATTACCTATCACTTTCAACACCCGTAGGGATCTTTTTATATGCAGTTTTGGTTATCCTTTTTCCTTTTTTTACAGTTACATCCAGATTAACCCAGAACGGTTAG", "aa": "MQTVSSPKQKLNFGQRLLTLLQNRDFMVSLVLTVVLLILFRVLAIIPLPGIRINESVLDRNSNDFFSLFNLLGGGGLNQLSLFAVGISPYISAQIIMQLLSTDLIPPLSKLVNSGEVGRRKIEMITRIITLPFALVQAFAVIQIATNASTGSSPISLANSGSEFIAFYIITMTAGTYMAVFLGDTISKKGVGNGITLLILSGILSQLPQGFIAAYNVLSGIVITLTPQLTAAISFFIYFLAFLVLLFATTFITQATRKIPIQQSGQGLVSEVKTLPYLPIKVNAAGVIPVIFASSIMSIPVTIAQFQPQTESRWFVEDYLSLSTPVGIFLYAVLVILFPFFTVTSRLTQNG", "aa_hexdigest": "bd1e41573ddfa22e8f5f3cfb331d9ecb", "start_type": "ATG", "rbs_motif": null, "genes": [ "secY" ], "truncated": "3-prime", "pseudogene": { "start": 199265, "stop": 200622, "observations": { "deletion": [ 200276 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47416", "query_cov": 0.9715099715099715, "subject_cov": 0.7178947368421053, "identity": 0.991, "score": 614.0, "evalue": 6.51e-219, "gene_start": 1, "gene_end": 341, "reference_start": 1, "reference_end": 341, "reference_sequence": "MQTVSSPKQKLNFGQRLLTLLQNRDFMVSLVLTVVLLILFRVLAIIPLPGIRINESVLDRNSNDFFSLFNLLGGGGLNQLSLFAVGISPYISAQIIMQLLSTDLIPPLSKLVNSGEVGRRKIEMITRIITLPFALVQAFAVIQIATNAGTGSSPISLANSGSEFIAFYIIAMTAGTYMAVFLGDTISKKGVGNGITLLILSGILSQLPQGFIAAYNVLSGIVITLTPQLTAAISFFIYFLAFLVLLFATTFITQATRKIPIQQSGQGLVSEVKTLPYLPIKVNAAGVIPVIFASSIMSIPVTIAQFQPQTESRWFVEDYLSLSTPVGIFLYAVLVILFSFFYSYIQINPERLAKNFEKSGRFIPGIRPGNDTEKHIARVLIRINFIGAPFLTVIAIIPYIVSYFIRLPNSLSLGGTGIIIIVTAVVEFISALRSAATATNYQQLRRNLAIEVQQTAKQDSLEQLQKEAPGIGNLW" }, "paralog": false, "identity": 0.995575221238938, "score": 845.0, "evalue": 2e-312, "description": "Frameshift due to deletion around 200276.", "psc": { "uniref90_id": "UniRef90_P47416", "gene": "secY", "product": "preprotein translocase subunit SecY", "uniref50_id": "UniRef50_O52351", "cog_id": "COG0201", "cog_category": "U", "go_ids": [ "GO:0005048", "GO:0005886", "GO:0006616", "GO:0008320", "GO:0015031", "GO:0016020", "GO:0031522", "GO:0043952" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47416", "UniRef:UniRef50_O52351", "COG:COG0201", "COG:U", "GO:0005048", "GO:0005886", "GO:0006616", "GO:0008320", "GO:0015031", "GO:0016020", "GO:0031522", "GO:0043952" ] }, "pscc": { "uniref50_id": "UniRef50_O52351", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52351" ], "product": "Protein translocase subunit SecY" } }, "id": "KMADJIBOEG_402", "locus": "GCA000292445_00366" }, { "type": "cds", "sequence": "contig_1", "start": 200398, "stop": 200691, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAATAAGGATTAACTTTATAGGTGCTCCTTTTTTAACTGTTATTGCTATTATCCCTTACATTGTTTCTTATTTCATTAGGTTACCTAACTCCTTGAGTTTAGGGGGGACGGGGATTATTATTATTGTTACTGCTGTAGTTGAATTTATCAGTGCACTGCGTTCAGCTGCTACTGCTACTAACTACCAACAACTAAGGAGAAACTTAGCAATTGAAGTGCAACAAACAGCTAAACAAGATAGTCTAGAGCAGCTTCAAAAAGAAGCACCAGGGATTGGTAACCTATGGTAG", "aa": "MLIRINFIGAPFLTVIAIIPYIVSYFIRLPNSLSLGGTGIIIIVTAVVEFISALRSAATATNYQQLRRNLAIEVQQTAKQDSLEQLQKEAPGIGNLW", "aa_hexdigest": "3232148e4db228b5a405c8f0988fcc3e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10657.388299999993, "isoelectric_point": 9.393446540832517 }, "id": "KMADJIBOEG_403", "locus": "GCA000292445_00367" }, { "type": "cds", "sequence": "contig_1", "start": 200685, "stop": 201329, "strand": "+", "frame": 3, "gene": "adk", "product": "Adenylate kinase", "db_xrefs": [ "COG:COG0563", "COG:F", "EC:2.7.4.3", "GO:0004017", "GO:0005524", "GO:0005737", "GO:0008270", "GO:0043231", "GO:0044209", "RefSeq:WP_014894189.1", "SO:0001217", "UniParc:UPI00027B399B", "UniRef:UniRef100_UPI00027B399B", "UniRef:UniRef50_P47417", "UniRef:UniRef90_P47417" ], "nt": "ATGGTAGCACAGTTTAATAAGTTCATTATCTTAGGACCCCCAGGGGCAGGAAAAGGTACAGTTTGTAAACTGCTTAGCAAAACAACTAAGTTAGTCCATATTGCTAGTGGTGATCTGTTTAGAGAAGCCATTAAAAACCAGAGTGCTGTTGGTAGAAAGATTGCAGCAATTATCAGTCACGGTGGTTATGTTGATGATACCACTACTAACCAGCTTGTTTATGAATATATCACTACGAATCCATTACCAAATGGTTTTATCTTAGATGGTTATCCAAGAACAGAGAACCAGCTTGATTTTCTAAATATTAAACTAACCATTGACATGGTCTTTGAACTAGTTGTTAGTGATCTGAATAAACTGATTACACGGATTGATAACAGGGTTATTTGTAACAACTGTAACAGTGTTTATAACTTGCTTTTTCAAAAACCACTAGTTGAAAATAGTTGTGATCAGTGTTCAGCTAAACTAGTGAAAAGGAGTGATGATAACAAAGCAGTGGTCAAAGCAAGAATGGAGTTATATCAACAAACAATTCAACCAATCCACACTTACTTTTTCAACAAACAACTTTTAGTACAAATTGATTGCTTTTTACCACTAGAAGAACAACTCAAGACAATCAAACAATTTATTAGATAA", "aa": "MVAQFNKFIILGPPGAGKGTVCKLLSKTTKLVHIASGDLFREAIKNQSAVGRKIAAIISHGGYVDDTTTNQLVYEYITTNPLPNGFILDGYPRTENQLDFLNIKLTIDMVFELVVSDLNKLITRIDNRVICNNCNSVYNLLFQKPLVENSCDQCSAKLVKRSDDNKAVVKARMELYQQTIQPIHTYFFNKQLLVQIDCFLPLEEQLKTIKQFIR", "aa_hexdigest": "1f71426226939aae27894031c97d3d21", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B399B", "ncbi_nrp_id": "WP_014894189.1", "uniref100_id": "UniRef100_UPI00027B399B", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B399B", "RefSeq:WP_014894189.1", "UniRef:UniRef100_UPI00027B399B" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B399B", "uniref90_id": "UniRef90_P47417" }, "psc": { "uniref90_id": "UniRef90_P47417", "gene": "adk", "product": "Adenylate kinase", "ec_ids": [ "2.7.4.3" ], "uniref50_id": "UniRef50_P47417", "cog_id": "COG0563", "cog_category": "F", "go_ids": [ "GO:0005737", "GO:0043231", "GO:0004017", "GO:0005524", "GO:0008270", "GO:0044209" ] }, "pscc": { "uniref50_id": "UniRef50_P47417", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47417" ], "product": "Adenylate kinase" }, "genes": [ "adk" ], "id": "KMADJIBOEG_404", "locus": "GCA000292445_00368" }, { "type": "cds", "sequence": "contig_1", "start": 201332, "stop": 202078, "strand": "+", "frame": 2, "gene": "map", "product": "type I methionyl aminopeptidase", "db_xrefs": [ "COG:COG0024", "COG:J", "EC:3.4.11.18", "GO:0004239", "GO:0005829", "GO:0006508", "GO:0046872", "GO:0070006", "KEGG:K01265", "RefSeq:WP_014894190.1", "SO:0001217", "UniParc:UPI00027B399C", "UniRef:UniRef100_UPI00027B399C", "UniRef:UniRef50_P47418", "UniRef:UniRef90_P47418" ], "nt": "ATGATCTATCTCAAATCTGCAAATGAAGTTGCAGGGATTAAAAAAGCATGTGCAATCTTCAAAGCAGTTAAGGCATATTTTACAATTGAAAAGTTACTTGGCAAAAAGTTGGTTACCATTGATCGTTTAATCAAACAATTCATTGAACAAAAACAAGCTAAATGTGCGTTTCATGGTTATCTAGGTTTCCCTGGTTTTAACTGTCTATCGTTAAACCAAACGGTTATCCATGGAGTTGCCGATCAAACTGTTTTTAAAGATAGTGATAAACTAACGCTTGACATTGGGATAGACTATCATGGTTATCTTTGTGATGCAGCTTTCACTTTACTTGGTAGTAAAGCTGATCCAAAGGCAGTAAAACTGTTAAATGATGTTGAACAAGCATTTAGTAAGGTAATTGAACCTGAGCTATTTGTTAACAACCGAATTGGTAATTTATCCAATGCGATCCAAACTTACTTTGAAAACAAGGGCTATTTTCTTGTCAAAGAGTTTGGGGGTCATGGTTGTGGGATTAAGATCCATGAAGATCCTTTAATCTTAAACTGGGGAGAGAAAAACCAGGGCATTAGGTTACAAGAGGGGATGGTAATCTGTATTGAACCGATGGTTATGACTGATAGTAGTGAGATAACAATGGCAGCTAACAACTGGAATGTACTAACTTTAAAAAGTAAGTTTAACTGTCATGTGGAACAGATGTATCACATCACAAACAACGGCTTTGAATGTTTAACTAACTAA", "aa": "MIYLKSANEVAGIKKACAIFKAVKAYFTIEKLLGKKLVTIDRLIKQFIEQKQAKCAFHGYLGFPGFNCLSLNQTVIHGVADQTVFKDSDKLTLDIGIDYHGYLCDAAFTLLGSKADPKAVKLLNDVEQAFSKVIEPELFVNNRIGNLSNAIQTYFENKGYFLVKEFGGHGCGIKIHEDPLILNWGEKNQGIRLQEGMVICIEPMVMTDSSEITMAANNWNVLTLKSKFNCHVEQMYHITNNGFECLTN", "aa_hexdigest": "ee92b480c0a2991db1c528ad2fb7632d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B399C", "ncbi_nrp_id": "WP_014894190.1", "uniref100_id": "UniRef100_UPI00027B399C", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B399C", "RefSeq:WP_014894190.1", "UniRef:UniRef100_UPI00027B399C" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B399C", "uniref90_id": "UniRef90_P47418" }, "psc": { "uniref90_id": "UniRef90_P47418", "gene": "map", "product": "type I methionyl aminopeptidase", "ec_ids": [ "3.4.11.18" ], "uniref50_id": "UniRef50_P47418", "cog_id": "COG0024", "cog_category": "J", "kegg_orthology_id": "K01265", "go_ids": [ "GO:0004239", "GO:0005829", "GO:0006508", "GO:0046872", "GO:0070006" ] }, "pscc": { "uniref50_id": "UniRef50_P47418", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47418" ], "product": "Methionine aminopeptidase" }, "genes": [ "map" ], "id": "KMADJIBOEG_405", "locus": "GCA000292445_00369" }, { "type": "cds", "sequence": "contig_1", "start": 202078, "stop": 202290, "strand": "+", "frame": 1, "gene": "infA", "product": "translation initiation factor IF-1", "db_xrefs": [ "COG:COG0361", "COG:J", "GO:0003743", "GO:0005829", "GO:0006413", "GO:0019843", "GO:0043022", "KEGG:K02518", "RefSeq:WP_009885858.1", "SO:0001217", "UniParc:UPI000012D262", "UniRef:UniRef100_P47419", "UniRef:UniRef50_Q50298", "UniRef:UniRef90_P47419" ], "nt": "ATGAAAAACGATAAACTCTTTCTAACAGGTAAGATACTGGAAATTATCCATGGTGATAAGTACCGGGTGATGCTTGAAAACAATGTTGAGGTTGATGCACATCTAGCAGGTAAAATGAAGATGAAAAGAACCAAGATTCTCCCTGGGGATGTTGTTGAGGTGGAATTTTCTCCCTATGATTTGAAACTAGGTAGGATAACCCAAAGAAAATAA", "aa": "MKNDKLFLTGKILEIIHGDKYRVMLENNVEVDAHLAGKMKMKRTKILPGDVVEVEFSPYDLKLGRITQRK", "aa_hexdigest": "cb34f5de7123f10488fac58fbf88077f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012D262", "ncbi_nrp_id": "WP_009885858.1", "uniref100_id": "UniRef100_P47419", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012D262", "RefSeq:WP_009885858.1", "UniRef:UniRef100_P47419" ] }, "ips": { "uniref100_id": "UniRef100_P47419", "uniref90_id": "UniRef90_P47419" }, "psc": { "uniref90_id": "UniRef90_P47419", "gene": "infA", "product": "translation initiation factor IF-1", "uniref50_id": "UniRef50_Q50298", "cog_id": "COG0361", "cog_category": "J", "kegg_orthology_id": "K02518", "go_ids": [ "GO:0003743", "GO:0005829", "GO:0006413", "GO:0019843", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_Q50298", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50298" ], "product": "Translation initiation factor IF-1" }, "genes": [ "infA" ], "id": "KMADJIBOEG_406", "locus": "GCA000292445_00370" }, { "type": "cds", "sequence": "contig_1", "start": 202414, "stop": 202671, "strand": "+", "frame": 1, "gene": null, "product": "30S ribosomal protein S13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A535XWH1" ], "nt": "ATGGCACGAATCTTAGGGATTGATATCCCCAACCAAAAACGGATCGAGATAGCTTTAACATACATCTTTGGGATTGGTTTGTCAAGTGCAAAAACAATCTTAAAAAAAGCAAAGATTAACCCTGATAAACGCGTTAAAGATCTGAGTGAAGAGGAACTTGTTGCGATTAGAAACGCAGCAAGCGGTTACAAGATTGAGGGTGATTTGAGAAGAGAGATTGCTTTAAACATCAAACACCTAACAGAGATCGGTTCTTGA", "aa": "MARILGIDIPNQKRIEIALTYIFGIGLSSAKTILKKAKINPDKRVKDLSEEELVAIRNAASGYKIEGDLRREIALNIKHLTEIGS", "aa_hexdigest": "11c6e5322ee6e5b4fc1e95a948b562ec", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0031B5D25D", "query_cov": 1.011764705882353, "subject_cov": 0.9885057471264368, "identity": 0.57, "score": 95.5, "evalue": 4.27e-24, "valid": false, "product": "30S ribosomal protein S13", "uniref50_id": "UniRef50_A0A535XWH1", "kegg_orthology_id": "K02952" }, "pscc": { "uniref50_id": "UniRef50_A0A535XWH1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A535XWH1" ], "product": "30S ribosomal protein S13" }, "genes": [], "id": "KMADJIBOEG_407", "locus": "GCA000292445_00371" }, { "type": "cds", "sequence": "contig_1", "start": 202974, "stop": 203153, "strand": "+", "frame": 3, "gene": "rpsK", "product": "30S ribosomal protein S11", "db_xrefs": [ "COG:COG0100", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "KEGG:K02948", "SO:0001217", "UniRef:UniRef50_P47422", "UniRef:UniRef90_P47422" ], "nt": "GTGGCTAAAACTGTGAAAGAGATGGGAATGGGGAGTGTTAAGATGTATCTGAAGGGAACAGGTAGAGGAAAAGACACCACGATTAGAAGCTTTGCTAATGCTGGGATTACGATCACAGAAATCAATGAAAAAACCCCTATTCCCCACAATGGCTGCAAGCCTCCTAAGCGTCCGCGCTAA", "aa": "MAKTVKEMGMGSVKMYLKGTGRGKDTTIRSFANAGITITEINEKTPIPHNGCKPPKRPR", "aa_hexdigest": "1ffbae663cd83fb40a4b2d12bf80cca3", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_K2BZH6", "query_cov": 0.9322033898305084, "subject_cov": 0.8461538461538461, "identity": 0.509, "score": 67.4, "evalue": 1.11e-13, "valid": false, "product": "Small ribosomal subunit protein uS11", "uniref50_id": "UniRef50_K2BZH6" }, "genes": [ "rpsK" ], "truncated": "5-prime", "pseudogene": { "start": 202788, "stop": 203153, "observations": { "mutation": [ 202896 ], "stop": [ 202896 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47422", "query_cov": 1.0, "subject_cov": 0.48760330578512395, "identity": 0.983, "score": 123.0, "evalue": 2.97e-35, "gene_start": 1, "gene_end": 59, "reference_start": 63, "reference_end": 121, "reference_sequence": "MAKKKKINVPSGLIHVSCSPNNTIVSATDPSGNVLCWASSGTVGFKGFRKKTPYSAGVAADKVAKTVKEMGMGSVKMYLKGTGRGKDTTIRSFANAGITITEINEKTPIPHNGCKPPKRPR" }, "paralog": false, "identity": 0.9917355371900827, "score": 244.0, "evalue": 1.92e-85, "description": "Internal stop codon at 202896. Nonsense mutation around 202896.", "psc": { "uniref90_id": "UniRef90_P47422", "gene": "rpsK", "product": "30S ribosomal protein S11", "uniref50_id": "UniRef50_P47422", "cog_id": "COG0100", "cog_category": "J", "kegg_orthology_id": "K02948", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47422", "UniRef:UniRef50_P47422", "COG:COG0100", "COG:J", "KEGG:K02948", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P47422", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47422" ], "product": "Small ribosomal subunit protein uS11" } }, "id": "KMADJIBOEG_408", "locus": "GCA000292445_00372" }, { "type": "cds", "sequence": "contig_1", "start": 203171, "stop": 203500, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01193.29" ], "nt": "ATGGAAAAATTTTTAAAGTACGAAATTAAGGTTAACAACAACCAACCAACCAACACTAACCCCAACTATGGGATCTTTGAAGTAGCACCGTTAGAATCAGGGTTTGGAATTACCATTGGTAATGCGATGCGCCGAGTGTTACTTAGTTGTATCCCAGGTGCTAGTGTGTTTGCCATTGCCATTAGTGGGGTGAAACAAGAGTTTAGTAATGTGGAGGGTGTTTTGGAAGATGTGACTGAAATGGTGTTAAACTTCAAGCAACTGGTGGTGAGAATCTCTGATCTTTTGTTTGAAGATGGGGAGATGATCGAACCACCCTTAGAAAGGTGA", "aa": "MEKFLKYEIKVNNNQPTNTNPNYGIFEVAPLESGFGITIGNAMRRVLLSCIPGASVFAIAISGVKQEFSNVEGVLEDVTEMVLNFKQLVVRISDLLFEDGEMIEPPLER", "aa_hexdigest": "b0289ee9d10a5f7ece465eca25a24da6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01193.29", "name": "RNA_pol_L", "length": 43, "aa_cov": 0.3944954128440367, "hmm_cov": 0.6976744186046512, "evalue": 2.353270267407481e-10, "score": 35.22905731201172, "start": 30, "stop": 72 } ], "seq_stats": { "molecular_weight": 12135.856599999996, "isoelectric_point": 4.564591789245606 }, "id": "KMADJIBOEG_409", "locus": "GCA000292445_00373" }, { "type": "cds", "sequence": "contig_1", "start": 203576, "stop": 204157, "strand": "+", "frame": 2, "gene": "rpoA", "product": "DNA-directed RNA polymerase subunit alpha", "db_xrefs": [ "COG:COG0202", "COG:K", "EC:2.7.7.6", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0005737", "GO:0006351", "GO:0046983", "SO:0001217", "UniRef:UniRef50_P47423", "UniRef:UniRef90_P47423" ], "nt": "GTGATTAATAAGGACCTTTATCTCTTCTCTTTACAAAAGGACATGAAACTAACAGTCAGTGTTTATGTTAAACAGGGTAGGGGCTTTACTAGCTTTCTTGAAAACAGAGAATTGATCAATTCGCTTGGCATTATTGCTACAGATGCTAACTTTTCCCCGGTTTTACACTGTGGTTATGAAGTTCAAGAGGTGAAAACTTCCAAACAAAAGTTAACTGACCATCTCACCTTTAAGATTGCTACTAACGGTGCAATTAAAGCAGTGGATGCGTTTGCTATGGCAGCAAAGATCCTAATTGAACACTTAAACCCAATTGTAAGTGTCAATGAGTCAATTAAGAATTTAACAATTATCCAAGAGAAAGCAGAGGAAAGAAAGGTGAAATCATTTGCCAAGCAAATTGAAGAACTTGACTTTACTGTTAGAACCTTTAACTGTTTGAAAAGAAGTGGGATCCACACACTCCAAGAGTTACTATCAAAGTCATTAACTGACATTAGAGAGATTAGAAACCTAGGTAAGAAATCAGAACGGGAGATTATCAAAAAGGTGCAAGAGTTAGGTTTAAAATTCCGTTTTTAA", "aa": "MINKDLYLFSLQKDMKLTVSVYVKQGRGFTSFLENRELINSLGIIATDANFSPVLHCGYEVQEVKTSKQKLTDHLTFKIATNGAIKAVDAFAMAAKILIEHLNPIVSVNESIKNLTIIQEKAEERKVKSFAKQIEELDFTVRTFNCLKRSGIHTLQELLSKSLTDIREIRNLGKKSEREIIKKVQELGLKFRF", "aa_hexdigest": "151359c7f907dc2c0019ae7e82de4370", "start_type": "GTG", "rbs_motif": null, "genes": [ "rpoA" ], "truncated": "5-prime", "pseudogene": { "start": 203276, "stop": 204154, "observations": { "mutation": [ 203498 ], "stop": [ 203498 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47423", "query_cov": 0.9896373056994818, "subject_cov": 0.5823170731707317, "identity": 1.0, "score": 361.0, "evalue": 8.22e-124, "gene_start": 2, "gene_end": 192, "reference_start": 137, "reference_end": 327, "reference_sequence": "MEKFLKYEIKVNNNQPTNTNPNYGIFEVAPLESGFGITIGNAMRRVLLSCIPGASVFAIAISGVKQEFSNVEGVLEDVTEMVLNFKQLVVRISDLLFEDGEMIEPPLERWPVLKVTAEKKGAVYAKDLECPAGFEVINKDLYLFSLQKDMKLTVSVYVKQGRGFTSFLENRELINSLGIIATDANFSPVLHCGYEVQEVKTSKQKLTDHLTFKIATNGAIKAVDAFAMAAKILIEHLNPIVSVNESIKNLTIIQEKAEERKVKSFAKQIEELDFTVRTFNCLKRSGIHTLQELLSKSLTDIREIRNLGKKSEREIIKKVQELGLKFRS" }, "paralog": false, "identity": 0.9965753424657534, "score": 558.0, "evalue": 6.69e-204, "description": "Internal stop codon at 203498. Nonsense mutation around 203498.", "psc": { "uniref90_id": "UniRef90_P47423", "gene": "rpoA", "product": "DNA-directed RNA polymerase subunit alpha", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P47423", "cog_id": "COG0202", "cog_category": "K", "go_ids": [ "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0005737", "GO:0006351", "GO:0046983" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47423", "EC:2.7.7.6", "UniRef:UniRef50_P47423", "COG:COG0202", "COG:K", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0005737", "GO:0006351", "GO:0046983" ] }, "pscc": { "uniref50_id": "UniRef50_P47423", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47423" ], "product": "DNA-directed RNA polymerase subunit alpha" } }, "id": "KMADJIBOEG_410", "locus": "GCA000292445_00374" }, { "type": "cds", "sequence": "contig_1", "start": 204215, "stop": 204541, "strand": "+", "frame": 2, "gene": "rplQ", "product": "50S ribosomal protein L17", "db_xrefs": [ "COG:COG0203", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "KEGG:K02879", "SO:0001217", "UniRef:UniRef50_P47424", "UniRef:UniRef90_P47424" ], "nt": "ATGACAGTGCGTCAGCAAGTGAGTGCAGTGTTAAGTTATGGAAAGATTCAAACCACTTTAAAAAAAGCTAAGAACACCCAAAAAAGGTTAGAGAAGATTATTACCATTGCTAAAGTTGATAACTTTAACAACCGCAGGGCTGTTAAAAAGTGGTTATTAAATACCAATTCATTAGATGTAGATCAACTCACAAACCACCTTTTTAAAAAAGTAGCGCCACGTTTTTTAAAGCGTAATGGTGGTTATAGTAGAGTGTTAAAGTTGGGAGTTAGAAGGGGTGATAGTACTGAAATGGCGATCTTACAGCTGATAGATGCTACCAACTAA", "aa": "MTVRQQVSAVLSYGKIQTTLKKAKNTQKRLEKIITIAKVDNFNNRRAVKKWLLNTNSLDVDQLTNHLFKKVAPRFLKRNGGYSRVLKLGVRRGDSTEMAILQLIDATN", "aa_hexdigest": "7a02b700a388aeaa2722ac9241ee35b4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47424", "query_cov": 1.0, "subject_cov": 0.8780487804878049, "identity": 1.0, "score": 208.0, "evalue": 7.13e-68, "valid": true, "gene": "rplQ", "product": "50S ribosomal protein L17", "uniref50_id": "UniRef50_P47424", "cog_id": "COG0203", "cog_category": "J", "kegg_orthology_id": "K02879", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47424", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47424" ], "product": "Large ribosomal subunit protein bL17" }, "genes": [ "rplQ" ], "id": "KMADJIBOEG_411", "locus": "GCA000292445_00375" }, { "type": "cds", "sequence": "contig_1", "start": 204528, "stop": 205340, "strand": "+", "frame": 3, "gene": "ecfA1", "product": "energy-coupling factor transporter ATPase", "db_xrefs": [ "COG:COG1122", "COG:PR", "EC:7.-.-.-", "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190", "SO:0001217", "UniRef:UniRef50_P47425", "UniRef:UniRef90_P47425" ], "nt": "ATGCTACCAACTAAACAAGCTGCTTGTAGTTTTATTAATGTTGCTTTTTCCTATAATGAACTGCCATTAATTAGGGAACTATCTTTTAGTGTTTATGAGGGGGAATATGTTTGTATTGTTGGTCATAATGGCAGTGGTAAATCAACCATTTCCAAACTGTTAACAGGGTTATTAAAGCCCCAGGCAGGTGAGATTAAGATCTTTGGTAAAACAGTTGATTTTGATAATGTTAGTTACTTGAGAAATAACATTGGGATCATCTTTCAAAACCCTGATAACCAGTTTATTGGGATCACTGTTGAAGATGACATTGCCTTTGGGCTTGAAAACAAGTGTTTTTCAAGACAGAAGATAAAAGCCATTATTGATGAAGTTACCCTACAAACCCAAACTGATGGGTTTATTAAACAAGAACCCCATAACCTATCAGGGGGACAAAAACAACGGGTAGCAATTGCATCTGTTTTAGCACTAAATCCTGCTATTATCATCTTTGATGAATCAACTGCGATGTTAGATCCTAAAGCTAAAAAAACGATTAAGCAGTTTATGGTTAAACTAGCCAAACAGGGCAAGTGTGTGATCTCAATTACCCATGATATGGAAGAAGTTACTAAAGCTGATAAGGTGTTAGTAATGAATGAGGGCAAACTGATCAAACAAGGTAAACCTGTTGAAGTTTTCACTAGTGAACAAGAGTTACAAAAAATCCGTTTAGACATCCCTTTTTCACTCAGTCTTTCAACCAAGATAAAAGGGATCACTAGTACAATTGATTACCAAACCCTGATTAAATCAATTGCCAAGCTGTGA", "aa": "MLPTKQAACSFINVAFSYNELPLIRELSFSVYEGEYVCIVGHNGSGKSTISKLLTGLLKPQAGEIKIFGKTVDFDNVSYLRNNIGIIFQNPDNQFIGITVEDDIAFGLENKCFSRQKIKAIIDEVTLQTQTDGFIKQEPHNLSGGQKQRVAIASVLALNPAIIIFDESTAMLDPKAKKTIKQFMVKLAKQGKCVISITHDMEEVTKADKVLVMNEGKLIKQGKPVEVFTSEQELQKIRLDIPFSLSLSTKIKGITSTIDYQTLIKSIAKL", "aa_hexdigest": "cc90f493acaf5880dcd95a8d0d2d4f53", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47425", "query_cov": 1.0, "subject_cov": 0.9854014598540146, "identity": 0.996, "score": 520.0, "evalue": 4.85e-186, "valid": true, "gene": "ecfA1", "product": "energy-coupling factor transporter ATPase", "ec_ids": [ "7.-.-.-" ], "uniref50_id": "UniRef50_P47425", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190" ] }, "pscc": { "uniref50_id": "UniRef50_P47425", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47425" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA1" }, "genes": [ "ecfA1" ], "id": "KMADJIBOEG_412", "locus": "GCA000292445_00376" }, { "type": "cds", "sequence": "contig_1", "start": 205337, "stop": 206251, "strand": "+", "frame": 2, "gene": "ecfA2", "product": "energy-coupling factor transporter ATPase", "db_xrefs": [ "COG:COG1122", "COG:PR", "EC:3.6.3.-", "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190", "RefSeq:WP_009885865.1", "SO:0001217", "UniParc:UPI00001393F5", "UniRef:UniRef100_P47426", "UniRef:UniRef50_Q50293", "UniRef:UniRef90_P47426" ], "nt": "GTGAAAAAAAAGATAGTCCCAATTAACCCTTTAAAAGCAGATGAGATTTTAGCAGTTAGTCACTTATCATGTGTTTTTAACAGTAAAACTAACAATCCCATTAAGGTGATTGATGATTTTTCCTATACGTTTCAAAAGAACCAAATTTACTGTATTATTGGTGATAGTGGCAGTGGTAAATCAACCCTTGTTAACCACTTCAATGGGTTGATAAAACCCAACCAAGGTGATATTTGGGTTAAAGATATCTATATTGGTGCTAAACAACGCAAGATTAAGAACTTTAAAAAACTGCGAAAAACTATCTCAATTGTTTTCCAGTTTCCTGAGTACCAATTGTTTAAAGATACCGTGGAAAAAGACATTATGTTTGGTCCAGTAGCATTAGGTCAATCCAAGTATGATGCGCGCCAAAAAGCGGCTTATTATCTGGAGATGATGGGGTTAAAATACCCCTTTTTAGAACGTAATCCCTTTGAATTGAGTGGGGGGCAGAAAAGAAGGGTAGCGATTGCTGGTATACTTGCAATTGAACCAGAAATTCTAATCTTTGATGAACCAACTGCTGGGCTTGATCCTGAAGGGGAAAGGGAGATGATGCAGTTAATTAAAACTGCCAAACAACAACAAAGAACGGTATTTATGATCACCCACCAGATGGAAAATGTCCTTGAGGTGGCTGATGTGGTTTTGGTTTTAGCTAAGGGTAAACTAGTAAAAGCTGCTAGTCCATATGAAGTGTTTATGGACCAAACTTTCCTTGAAAAAACAACGATTGTTCTCCCCCCTGTGATCCAAGTGATCAAAGATCTAATTGCGATTAATGCTCACTTTAATAAGTTAATTGAGTTGCAACCAAAGAATCTAGAACAGCTTGCATCAGCAATTAACAAGACTATAGCAAACCATGGATAA", "aa": "MKKKIVPINPLKADEILAVSHLSCVFNSKTNNPIKVIDDFSYTFQKNQIYCIIGDSGSGKSTLVNHFNGLIKPNQGDIWVKDIYIGAKQRKIKNFKKLRKTISIVFQFPEYQLFKDTVEKDIMFGPVALGQSKYDARQKAAYYLEMMGLKYPFLERNPFELSGGQKRRVAIAGILAIEPEILIFDEPTAGLDPEGEREMMQLIKTAKQQQRTVFMITHQMENVLEVADVVLVLAKGKLVKAASPYEVFMDQTFLEKTTIVLPPVIQVIKDLIAINAHFNKLIELQPKNLEQLASAINKTIANHG", "aa_hexdigest": "4db8cad8c5695dfd3904d297caf5ae55", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001393F5", "ncbi_nrp_id": "WP_009885865.1", "uniref100_id": "UniRef100_P47426", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001393F5", "RefSeq:WP_009885865.1", "UniRef:UniRef100_P47426" ] }, "ips": { "uniref100_id": "UniRef100_P47426", "uniref90_id": "UniRef90_P47426" }, "psc": { "uniref90_id": "UniRef90_P47426", "gene": "ecfA2", "product": "energy-coupling factor transporter ATPase", "ec_ids": [ "3.6.3.-" ], "uniref50_id": "UniRef50_Q50293", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190" ] }, "pscc": { "uniref50_id": "UniRef50_Q50293", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50293" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA2" }, "genes": [ "ecfA2" ], "id": "KMADJIBOEG_413", "locus": "GCA000292445_00377" }, { "type": "cds", "sequence": "contig_1", "start": 206244, "stop": 206579, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAACTTTATTAATGGCTATATCCCAAGAAACAGCTTTGTTCACAAGCTGCATCCAACTACTAAACTAGTAATCTTTTTACTGTTAGTTATCTTGGTATTTGTACCAATTGGCTTTGTTTTTCAAAGTGTTATCTTTCTTTTTGTTACCTTTGTTTTTTTTATTGCTAAACTCCCGGGGCGGTTTTACAGTTCAGCAATTAAGTCAATTACGCTGTTATTTCTCTTGTTGTTATTTGTAAACTGGTTTACCTTTCGTGATCCAGGGTTTTATCTTACTAGTGATCAACTTAACAGTTTACCAGCCATTGATAACAGCAAGTTTAGCTTTTGA", "aa": "MDNFINGYIPRNSFVHKLHPTTKLVIFLLLVILVFVPIGFVFQSVIFLFVTFVFFIAKLPGRFYSSAIKSITLLFLLLLFVNWFTFRDPGFYLTSDQLNSLPAIDNSKFSF", "aa_hexdigest": "afd990e90f8e6db4d241d4ea86bf3ffc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12867.17819999998, "isoelectric_point": 9.774519157409667 }, "id": "KMADJIBOEG_414", "locus": "GCA000292445_00378" }, { "type": "cds", "sequence": "contig_1", "start": 206589, "stop": 207050, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAACTATCAAGATAATGTTTTTTCCCAGGTTTTTGCTTTTAACAGGGGTAATTTAACCAACTTAAATCAACTTGATTTTTTCTATAAAGCTAACAATGCTGATAGTTACACCAAAGTAAAGGGCATTGATAGTTTAGCAAGTATGCTAGCAAACAATGGCAATGGTTTAAGCAAAGACAAAATTCTGAGTGCTTTTTTAGATCACAATTTAAACCTTTATTTAGCGAGAAGTTGGGGGGCAAATTTTGCTGGGTTTGTTGTTGATTTTAACCCAACAACCCAACTCTTTAAACTCACTCCTTTTCTAGCAAATGCTAGTTATGTTTTAACGTTAAGAGCAGTTATCTTAGCATTCTATGTCACCCAAAAGATCCTAATTATGATCTTATTTGCAACTGTACTCACTTCCACTTCAAGTTCAGTTGAACTAGCATATGGGATTGAAAGGTTATTATGA", "aa": "MFNYQDNVFSQVFAFNRGNLTNLNQLDFFYKANNADSYTKVKGIDSLASMLANNGNGLSKDKILSAFLDHNLNLYLARSWGANFAGFVVDFNPTTQLFKLTPFLANASYVLTLRAVILAFYVTQKILIMILFATVLTSTSSSVELAYGIERLL", "aa_hexdigest": "331d99e514f5958aa36d2962e941fdec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17091.436999999994, "isoelectric_point": 8.891173362731934 }, "id": "KMADJIBOEG_415", "locus": "GCA000292445_00379" }, { "type": "cds", "sequence": "contig_1", "start": 207090, "stop": 207506, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCATTGCCATTGCCATTAGGTTTGTCCCTTCCTTGTTACTAGAATCACAACGGATCTTAAATGCCCAAGCATCCAGGGGTTTAGACTTTCGAAATGGGGGATTTTTAGTGAAGATGCGTTCACTCTCTTCGTTAGTAGTACCAATGGTCTCCATTGCATTTAGGAATGCCTCTGAACTTGCTAGTGCAATGGAAGCAAGGGGTTATCACCCTGCAAAGAAGCGCAGTAGTTATAGACAATACAAAATCACTTGGATTGATATTTTAGCGTTATTTTTGGTTTTTGCTTGGTTTGTTGTGATTATCTTTTTAACTATTAGAGGAGCGGTCTTTTTGGATATAGGTACACCAGAATGGTTACTAACAGGAAAGATTAATGAACAGGTAATCAGGGATCTGAAGGTAAGTGGCTAG", "aa": "MTIAIAIRFVPSLLLESQRILNAQASRGLDFRNGGFLVKMRSLSSLVVPMVSIAFRNASELASAMEARGYHPAKKRSSYRQYKITWIDILALFLVFAWFVVIIFLTIRGAVFLDIGTPEWLLTGKINEQVIRDLKVSG", "aa_hexdigest": "0a5798fb69dc61b28258607b73049c92", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15532.232999999984, "isoelectric_point": 10.462010002136228 }, "id": "KMADJIBOEG_416", "locus": "GCA000292445_00380" }, { "type": "cds", "sequence": "contig_1", "start": 207589, "stop": 208233, "strand": "+", "frame": 1, "gene": "truA", "product": "tRNA pseudouridine(38-40) synthase TruA", "db_xrefs": [ "COG:COG0101", "COG:J", "EC:5.4.99.12", "GO:0001522", "GO:0003723", "GO:0009451", "GO:0009982", "GO:0031119", "GO:0160147", "SO:0001217", "UniRef:UniRef50_P47428", "UniRef:UniRef90_P47428" ], "nt": "TTGGAACAAAGTTTTTCATTAATCATTGGCAGAAAGATAAAGGTAATTGGTTCAGGTAGAACTGATAAAGGGGTACATGCCATCAACCAAACCTTTCATGTTGATATTAATGGTGAAATTAATCTCAATTTGTTAATTAGAAAAATTAACCAGTTGATTAAGCCCCACTGTATAGTTAAAACCTTGGTATTGGTTAACGATAGCTTTCATGCGCGGTTTCAAGTTAAAACTAAGGTGTATGAATATCTGATTAACTGTGGGAATTTAAACCCGTTGCAATTTAACTATGTTTGGCAGTTAAACCAGCAATTGGATCTTGAAAAACTCAAAGCTGATGCCACTTTATTTTTAGGTAAGAAAAACTTTCTTAGCTTCAGTAGTTCGATTCACACTGATTCAATTCGCACAATTAGTAAAATTACCATACAAAAAGAAACTAACCAACTAGTTAGACTAACTTTTTTTGGCAGTGGTTTTCTCAGGAGTCAAGTGAGGATGATAGTTGCTTGTTTAGTGAATTTAAACACTAATAAAATGGCACTTGAAACAGTTGCAAAATTGTTTGAACACCCCAAGAAAGGGAGTTGTGTTGTTAAAGCCCCTAGTTGTGGTTTGTATCTGAAAACAGTGGTATATGAAAAATAG", "aa": "MEQSFSLIIGRKIKVIGSGRTDKGVHAINQTFHVDINGEINLNLLIRKINQLIKPHCIVKTLVLVNDSFHARFQVKTKVYEYLINCGNLNPLQFNYVWQLNQQLDLEKLKADATLFLGKKNFLSFSSSIHTDSIRTISKITIQKETNQLVRLTFFGSGFLRSQVRMIVACLVNLNTNKMALETVAKLFEHPKKGSCVVKAPSCGLYLKTVVYEK", "aa_hexdigest": "746a0dd3964a5b1fb4c3cce16223b794", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47428", "query_cov": 1.0, "subject_cov": 0.8770491803278688, "identity": 0.995, "score": 422.0, "evalue": 6.17e-149, "valid": true, "gene": "truA", "product": "tRNA pseudouridine(38-40) synthase TruA", "ec_ids": [ "5.4.99.12" ], "uniref50_id": "UniRef50_P47428", "cog_id": "COG0101", "cog_category": "J", "go_ids": [ "GO:0001522", "GO:0003723", "GO:0009451", "GO:0009982", "GO:0031119", "GO:0160147" ] }, "pscc": { "uniref50_id": "UniRef50_P47428", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47428" ], "product": "tRNA pseudouridine synthase A" }, "genes": [ "truA" ], "id": "KMADJIBOEG_417", "locus": "GCA000292445_00381" }, { "type": "cds", "sequence": "contig_1", "start": 208223, "stop": 208867, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAGTTACAAGTGGGATCTATCAGTTTTATTAAACAACCAAAGCTTACAAGCAAATTTTTTAAAAATTCAAACAGTTAGTGAAGCGTTAATTAAAGCTTATAACAACGGGTTGTGTTTTACAAATAAAACTAGCTTTGAACAGTTTTTAGCAATCGATGATAAGTTCACTGAACTTGAAAATCGTTACACTAACTACCTTTATAACAAGCAGAATGAAAATAACTTGGATAAGGAGGTTAATGATGCAATTTTTGCATACCAGAGTTTTAAAAATAACCATAACCTTGCTTTCAGTACACTGCAACAGGAGTTATATAACCATGAAAAACTCATTAAGGATTATTTAACTGATCCAAAGCTAGCGGTTTACAAGCGCAACTTAATGTTAGTTTTTCGCGATAAACCCCACCAACTATCTAGTCAAACCCAGAGTTTATTGAGTCAAATTAACCCTTGTTTTAACCAAGCAGAACGGATTTTTAACATCCTTTCAACTGCTGATTTAAACTTGCAACCTGTTGTTTATCAAAACAAAAAATATCCGATTAACAGTGTGAGTGATTATCAGTCCTTACTTGAAAACACTAACAGGGGGATTAGAAAAGCTTGTTATGAGAAGTGGATTGAAATTTATTGA", "aa": "MKNSYKWDLSVLLNNQSLQANFLKIQTVSEALIKAYNNGLCFTNKTSFEQFLAIDDKFTELENRYTNYLYNKQNENNLDKEVNDAIFAYQSFKNNHNLAFSTLQQELYNHEKLIKDYLTDPKLAVYKRNLMLVFRDKPHQLSSQTQSLLSQINPCFNQAERIFNILSTADLNLQPVVYQNKKYPINSVSDYQSLLENTNRGIRKACYEKWIEIY", "aa_hexdigest": "d2d2a3282c9a19d3b832092f49baa0e4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25257.233700000015, "isoelectric_point": 8.274855613708496 }, "id": "KMADJIBOEG_418", "locus": "GCA000292445_00382" }, { "type": "cds", "sequence": "contig_1", "start": 208898, "stop": 209254, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAAATTACATCCAACTAGAGAACTTCGCTAAACTAAAGAACCATCCTAGTTACATTGCCCAAACTGCTTTTAATGATGAGATTGAAGTTGGTTTTATTGATTTTGTTTACCAACAAGTAGCTCAGTTTGCCAAAACCTTTCAAGCATTTATTCGCTTAAAAAAACAGATTTATAAACATGTTTTAAAAGTCAATAAAGTTGAACCATATGATCTTACCCTAACACTTTTTAAAACTAAAAAACCATACACGATTGAACAAGCTAAACAAGATGCACTAAAAGTTTTAGATCTACTTGGTGACAACTACATCAAGATAGTTAAAAAGGCTTTTAATGAAAACTGGATTGATTGA", "aa": "MENYIQLENFAKLKNHPSYIAQTAFNDEIEVGFIDFVYQQVAQFAKTFQAFIRLKKQIYKHVLKVNKVEPYDLTLTLFKTKKPYTIEQAKQDALKVLDLLGDNYIKIVKKAFNENWID", "aa_hexdigest": "4b9f6bb5dd4bb3c4504aef4959db2cf0", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13967.019899999981, "isoelectric_point": 8.981558036804202 }, "id": "KMADJIBOEG_419", "locus": "GCA000292445_00383" }, { "type": "cds", "sequence": "contig_1", "start": 209330, "stop": 209974, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTTGATAAAACCAAATCATCACTAAATACGTTGGTACATGAACTTGGTCATTCAGTTCACTCTTGGTATGCATCACAACACCAATCCCAAAACATTGATCCTACTATCTTTTATGCTGAGATTGCTTCTATTGCCAATGAACTGTTGTTGTGTTATTATGAACTGCAACTTTATAAAAATAACCACAAGCAGTTAATTGCTAGTTTATTGAGTCAAATTAACCATTTTTTTGGCGCTACTACAAGACAAATAATGTTTTCACAATTTGAAAAAGATACGCTTGATTTAATCAGAGTTAACCAGAAACCTGACTTTAAAACTTTGATTAAAATTTATGCAAATACTGCGGTTAAATACCAAGGTTTTAAACCTGAAGTAGTTGCTAATAAACTAAAAAAGACCCAGTATCAAAAATCATTGTCACACATCATTGCTATCCCCCATTTTTATGCAGGTAACTTCTATGTTTACAAGTATGCCATTGGCCAAGTTGCAGGTATTTTAGTAGCTAAAAAAATTAATAGTGGTGATAAAAAGATGAAAGATAATTACTTTAAATTCCTCAGTTCAGGTTCTAGTTTAGCGCCACTTGAAACCATTAAACTCTTAGGGATTGACCTTACTTCACCCCAACCCTGA", "aa": "MNFDKTKSSLNTLVHELGHSVHSWYASQHQSQNIDPTIFYAEIASIANELLLCYYELQLYKNNHKQLIASLLSQINHFFGATTRQIMFSQFEKDTLDLIRVNQKPDFKTLIKIYANTAVKYQGFKPEVVANKLKKTQYQKSLSHIIAIPHFYAGNFYVYKYAIGQVAGILVAKKINSGDKKMKDNYFKFLSSGSSLAPLETIKLLGIDLTSPQP", "aa_hexdigest": "4a822fe44fffc50bcaf24404c970ad71", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24389.820999999996, "isoelectric_point": 9.419169425964355 }, "id": "KMADJIBOEG_420", "locus": "GCA000292445_00384" }, { "type": "cds", "sequence": "contig_1", "start": 210047, "stop": 210796, "strand": "+", "frame": 2, "gene": null, "product": "Type II methyltransferase M-MgeORF184P", "db_xrefs": [ "EC:2.1.1.72", "GO:0003677", "GO:0009007", "GO:0032259", "SO:0001217", "UniRef:UniRef50_Q50290", "UniRef:UniRef90_Q49400" ], "nt": "GTGCACCATTTTAACCGCGCTAAAAAGGCCAAGAATAACGAGTTTTTTACTTTAATTGATGAGATTGAAAACGAAGTAATTAACTACCAAAAGCAGTTTGCAAATAAAACCATTTTTTGTAACTGTAATGATGGTAAAAATTCCCATTTTTTTCAGTTTTTTCAAACTAACTTTAACCAGTTACAACTAAAAAAACTCATTGGGTTTAGTTTTAATAATCTCTCACAAGCTGACAAGTTCACTTTTGATGGAAATAAAGTAACTAAAACCAAATTAAAGGGTAATGGTGATTTTAGTTCTGATGAATCGATTGAAGTGTTAAAACAAGCAGATATAGTTGTAACAAATCCACCCTTTAGTTTGTTTCAAAGTTTCATTGATCTGTTAATACAACACAACAAGCAGTTTCTGGTTTTAGGGTTAAATGCAGCAGTTAGCTATAACCATATTTTTACCTACTTTAAAACTAACAAGTTGTGGTTTGGCTATACCGTTAATAAAACAATGAGTTTTTCAGTTAACAGTGACTATCAACTTTATAACCCCAAAACTAGTAACTTTTTTACAAAAAATGGCAAGTGTTTCCAAAAGATAGCAGGTATCTCTTGGTTTACTAATTTAGGAAAACCACATTATAACCCCTTTTTAAATACCAACTGTTTTTATAAAAACAACGAAAAAAACTATCCCAAGTTTGATTGGTATGATGCTATTTATGTCAACAAGATTAAAAACATCCCTATGGATTGA", "aa": "MHHFNRAKKAKNNEFFTLIDEIENEVINYQKQFANKTIFCNCNDGKNSHFFQFFQTNFNQLQLKKLIGFSFNNLSQADKFTFDGNKVTKTKLKGNGDFSSDESIEVLKQADIVVTNPPFSLFQSFIDLLIQHNKQFLVLGLNAAVSYNHIFTYFKTNKLWFGYTVNKTMSFSVNSDYQLYNPKTSNFFTKNGKCFQKIAGISWFTNLGKPHYNPFLNTNCFYKNNEKNYPKFDWYDAIYVNKIKNIPMD", "aa_hexdigest": "cddcbbd39b52f9ffb7733348c1fcaccb", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 210047, "stop": 211000, "observations": { "mutation": [ 210794 ], "stop": [ 210794 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49400", "query_cov": 1.0, "subject_cov": 0.7854889589905363, "identity": 1.0, "score": 513.0, "evalue": 2.74e-183, "gene_start": 1, "gene_end": 249, "reference_start": 1, "reference_end": 249, "reference_sequence": "MHHFNRAKKAKNNEFFTLIDEIENEVINYQKQFANKTIFCNCNDGKNSHFFQFFQTNFNQLQLKKLIGFSFNNLSQADKFTFDGNKVTKTKLKGNGDFSSDESIEVLKQADIVVTNPPFSLFQSFIDLLIQHNKQFLVLGLNAAVSYNHIFTYFKTNKLWFGYTVNKTMSFSVNSDYQLYNPKTSNFFTKNGKCFQKIAGISWFTNLGKPHYNPFLNTNCFYKNNEKNYPKFDWYDAIYVNKIKNIPMDWNGLMGVPLTFLNCYNPKQFELVDCLANPYATLDTLKTNAFVKLNQGDVRNVNGKRRYVRVIIKKQQI" }, "paralog": false, "identity": 0.9936908517350158, "score": 658.0, "evalue": 7.05e-243, "description": "Internal stop codon at 210794. Nonsense mutation around 210794.", "psc": { "uniref90_id": "UniRef90_Q49400", "product": "Type II methyltransferase M.MgeORF184P", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_Q50290", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0032259" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49400", "EC:2.1.1.72", "UniRef:UniRef50_Q50290", "GO:0003677", "GO:0009007", "GO:0032259" ] }, "pscc": { "uniref50_id": "UniRef50_Q50290", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50290" ], "product": "Type II methyltransferase M.MpnI" } }, "id": "KMADJIBOEG_421", "locus": "GCA000292445_00385" }, { "type": "cds", "sequence": "contig_1", "start": 210806, "stop": 211000, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGTTCCTTTAACCTTTTTAAACTGTTACAACCCCAAGCAGTTTGAATTAGTTGATTGTCTTGCTAACCCTTATGCTACCTTAGATACATTAAAAACAAATGCCTTTGTGAAATTAAATCAGGGTGATGTGAGAAATGTTAATGGTAAAAGAAGGTATGTAAGGGTAATAATTAAAAAACAACAAATTTAG", "aa": "MGVPLTFLNCYNPKQFELVDCLANPYATLDTLKTNAFVKLNQGDVRNVNGKRRYVRVIIKKQQI", "aa_hexdigest": "19e9acc1ba819a4c2e570ba6152ca2bd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7371.567700000002, "isoelectric_point": 9.757757377624511 }, "id": "KMADJIBOEG_422", "locus": "GCA000292445_00386" }, { "type": "cds", "sequence": "contig_1", "start": 211100, "stop": 211726, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTCAAACTATTTTTTTTATCAAGCAGTGTGTTGGGTCCAATTGCCCTATTTACAACTGCTTGTAGTGCTGTTTATAGGTTTGATCAAGTTGATGATGGCAAGATTAAACTAGCAACTGTAACTTCAGCTTCCGCTAGTGGCTCGCTTACTACTATCATCAGTAAATATAATTCACAAAAAGATCCTAATGATTATCCAGTGGAACTGGTTTCACTTGATAGTAGGGGCAGTTATTCTAATGGCAAAAAGGATCTGCAAGCTAAACTGCTAGCTAAGGATAAAAATAACTTTTATAACCTTACTTTTAACTATAGTGATGTAGTTTCAATCCTCTCAAGAAGCCAGATGGAGTTGAGCTTTGATACGGTTGATACTAGTAATTTTGATCCTAGTTTTCTTAGTTTTAACAATAACATTTCCAATGTTAATCCAAATAGCATCTATGCTTTACCTGCTACTGTATCAGGTGAAGTTTTAGTTTTAAATGGACCGGTGTTACATTACATTTTAAGTAGTGCTAAAAAAGATAGTAACACCACCCTTTCAACCCATTCAGCTAGCAATAATAGCAATAAGGGAACAATGGTTGTTGCTAGTGATAGTGAAACATCAAGTTTATGA", "aa": "MKFKLFFLSSSVLGPIALFTTACSAVYRFDQVDDGKIKLATVTSASASGSLTTIISKYNSQKDPNDYPVELVSLDSRGSYSNGKKDLQAKLLAKDKNNFYNLTFNYSDVVSILSRSQMELSFDTVDTSNFDPSFLSFNNNISNVNPNSIYALPATVSGEVLVLNGPVLHYILSSAKKDSNTTLSTHSASNNSNKGTMVVASDSETSSL", "aa_hexdigest": "45ade32ed9956687bfb8a4a4a7b370fd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22476.832100000014, "isoelectric_point": 6.079066658020018 }, "id": "KMADJIBOEG_423", "locus": "GCA000292445_00387" }, { "type": "cds", "sequence": "contig_1", "start": 211751, "stop": 211915, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCTCAAACTAATGAAACCCAAGTTTTAAAAAGTAATTCATCAGAATCTAACCAAACCCAAGCTAGTGATACAGAGATTAAAAAGATTTGGGGTGATTATCAAGAAGTTGATGGAGGGTTGAAAAATTACACCTTTAAAGCTAGTGTTTTTAACAATTGA", "aa": "MNAQTNETQVLKSNSSESNQTQASDTEIKKIWGDYQEVDGGLKNYTFKASVFNN", "aa_hexdigest": "2a354eb9ad66692db4e9e73bce5190c4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6060.453400000002, "isoelectric_point": 4.715101432800293 }, "id": "KMADJIBOEG_424", "locus": "GCA000292445_00388" }, { "type": "cds", "sequence": "contig_1", "start": 212021, "stop": 213205, "strand": "+", "frame": 2, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0005886", "GO:0009897", "SO:0001217", "UniRef:UniRef50_Q50289", "UniRef:UniRef90_P47431" ], "nt": "TTGGGAAATGCCTTATATACTGCTTTATTTGCTTCTGGAAAAGCTGATTACAATAACTTTCTTTTTAACATCAAAAACCAGCGAATTAATTTCAGTAACTTTTTTAATAAATCCTCAACTGCATTTCAAAACCTTAAAACTATCTTTAACAGCTTCAAATCCTTAATTGATCAAAACGGTTTAATCTCAAATGCACACTTTAACACCCCAGTTAATGACTATGCTAAGTTTAACCAATTAGCTTTTTACACCTCTTCAACTGCACGCTTTCCCTATTCATTTGCAAGTGATAGTGTAAAGCGTTTAATAGTTAATGACAAGACAATTGAAAACAAAAACAATAAGAGTGTTTTTGAGGTTAATTTAAGTAGTGATAGTGATAACAACAGTAACCTAATTGGTACTGTATCACTGGAAAATAGTAAACAAGTTTCACTCTATGAAAAGCAAGTGGATAGTAATAAACAAATTGGTGTTGATGCTTTGTTAATCAAAGATGAAACTTTAATCAACCATCTTAAGAGTTTAAAATCGCAAGTTAGTGCAAAAAGTGCTAGTGAAACTTCCCAAACAAAACAAAACAAAACCTTTTTAGCATTTACAACTGTGAATGCAGATCAAAAAGCAATCTTTGATGTTGGTAAACTTAATGGCAAAACTGCCAAAATCATTATTAATGCTACTGAAACAACTAATGCTAAGATTAGCACCTTACAAGAAAAAGAGGCAATAGTTCTAAAAGCACCCCAACGCTTTGAGAGCACTGATCCATTTCCTATTGCTTTAGTGCAAGGTCCTAGTTTAATAGGGATCCATGCAAATGAAAGAGAGGATATTGAAACCAAAAAGTTTGTGAATTGGTATCTGAATACTAAAGTCCAATGGGAAGAAAATTCTATTAAAACTCCTGCAGAATATGTAGCTGATAAAGCTTCTTATCTTTTACCTTTTAAAAATAGGCTAAATAATACTAACAGTTACAATGAGTTTGTTAAAACTGCAGTTAGTCAGTTTGCTGATAAAAATGTAACTAAATTTGCAGAACCTGCTGATTTTTTATCCAACAAAGTACGTGATGGTGTTAAGAGTAATTTAAATGCTGCAATTAACAACCACAGCATTGATTTTGATAGTTTTATCAATGATTTAACAGACTATCTTGGTAGTGATGTTAAAAACATCTAA", "aa": "MGNALYTALFASGKADYNNFLFNIKNQRINFSNFFNKSSTAFQNLKTIFNSFKSLIDQNGLISNAHFNTPVNDYAKFNQLAFYTSSTARFPYSFASDSVKRLIVNDKTIENKNNKSVFEVNLSSDSDNNSNLIGTVSLENSKQVSLYEKQVDSNKQIGVDALLIKDETLINHLKSLKSQVSAKSASETSQTKQNKTFLAFTTVNADQKAIFDVGKLNGKTAKIIINATETTNAKISTLQEKEAIVLKAPQRFESTDPFPIALVQGPSLIGIHANEREDIETKKFVNWYLNTKVQWEENSIKTPAEYVADKASYLLPFKNRLNNTNSYNEFVKTAVSQFADKNVTKFAEPADFLSNKVRDGVKSNLNAAINNHSIDFDSFINDLTDYLGSDVKNI", "aa_hexdigest": "e769c200c044d0f6d5da6b7c58913e25", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 211727, "stop": 213205, "observations": { "mutation": [ 211913 ], "stop": [ 211913 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47431", "query_cov": 0.9949238578680203, "subject_cov": 0.5592011412268189, "identity": 1.0, "score": 709.0, "evalue": 2.97e-252, "gene_start": 3, "gene_end": 394, "reference_start": 310, "reference_end": 701, "reference_sequence": "MKFKLFFLSSSVLGPIALFTTACSAVYRFDQVDDGKIKLATVTSASASGSLTTIISKYNSQKDPNDYPVELVSLDSRGSYSNGKKDLQAKLLAKDKNNFYNLTFNYSDVVSILSRSQMELSFDTVDTSNFDPSFLSFNNNISNVNPNSIYALPATVSGEVLVLNGPVLHYILSSAKKDSNTTLSTHSASNNSNKGTMVVASDSETSSLWTKLEAAAKMNAQTNETQVLKSNSSESNQTQASDTEIKKIWGDYQEVDGGLKNYTFKASVFNNWKDLNDFATRIAKSFTKLQTTTKKGEEVQAVFGIGSLENALYTALFASGKADYNNFLFNIKNQRINFSNFFNKSSTAFQNLKTIFNSFKSLIDQNGLISNAHFNTPVNDYAKFNQLAFYTSSTARFPYSFASDSVKRLIVNDKTIENKNNKSVFEVNLSSDSDNNSNLIGTVSLENSKQVSLYEKQVDSNKQIGVDALLIKDETLINHLKSLKSQVSAKSASETSQTKQNKTFLAFTTVNADQKAIFDVGKLNGKTAKIIINATETTNAKISTLQEKEAIVLKAPQRFESTDPFPIALVQGPSLIGIHANEREDIETKKFVNWYLNTKVQWEENSIKTPAEYVADKASYLLPFKNRLNNTNSYNEFVKTAVSQFADKNVTKFAEPADFLSNKVRDGVKSNLNAAINNHSIDFDSFINDLTDYLGSDVKNI" }, "paralog": false, "identity": 0.9451219512195121, "score": 858.0, "evalue": 2.14e-313, "description": "Internal stop codon at 211913. Nonsense mutation around 211913.", "psc": { "uniref90_id": "UniRef90_P47431", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_Q50289", "go_ids": [ "GO:0005886", "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47431", "UniRef:UniRef50_Q50289", "GO:0005886", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_Q50289", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50289" ], "product": "Uncharacterized lipoprotein MPN_199" } }, "id": "KMADJIBOEG_425", "locus": "GCA000292445_00389" }, { "type": "cds", "sequence": "contig_1", "start": 213232, "stop": 213594, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTACTAGTGCCTAAGGTGGTAGTGGAGTACCACCAACTTGATAAGAAGGTTGTCAAAGAGAGTTTGGAAGTGGAATCTTCTTCCTCCTTCAACCCCACCCAAGGGTTGAAGAAAGATAGTCCAATGAAGGATTCAAGTAAACAAGGGGAGAAACTCAGTGAAGCAACTTCATCGATGAGTAGTGGTGGGGCTACCTCTCCTCGCAAGGCCCTTAAGATAGAGGTGGAGAAAGGCAGTAAAGTCAACCAAGGCGAACTACAATCCAACGACTTTGCTAAAAAGCCGTTGCAGCATAAGAACAATAGTGGGACAGATGTGAAGTTGGATGCGAGTGGGGAGTTTGCTGACGACAAAGCCTGA", "aa": "MLLVPKVVVEYHQLDKKVVKESLEVESSSSFNPTQGLKKDSPMKDSSKQGEKLSEATSSMSSGGATSPRKALKIEVEKGSKVNQGELQSNDFAKKPLQHKNNSGTDVKLDASGEFADDKA", "aa_hexdigest": "cdd88b18ffb9d794534c6ba75a9885e1", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FCE6354", "query_cov": 0.9333333333333333, "subject_cov": 0.8484848484848485, "identity": 0.7859999999999999, "score": 151.0, "evalue": 3.87e-45, "valid": false, "product": "adhesin", "uniref50_id": "UniRef50_A0A7M3VDG5" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12952.373299999985, "isoelectric_point": 7.885015296936035 }, "id": "KMADJIBOEG_426", "locus": "GCA000292445_00390" }, { "type": "cds", "sequence": "contig_1", "start": 213827, "stop": 214231, "strand": "+", "frame": 2, "gene": null, "product": "Random genomic clone esh10, partial cds", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49206" ], "nt": "ATGACAACAATAACGGTATCTCCTTTTGAACAGGTGAAACCTAGCAATGGTAGCAACACGTTTAATCCCAATTCAGATGATAATAAAGTCACACCATCAGGTGGCTCCCCAGCCAAAAAAACCACCTATGATTTTTTACCCAACTCCATCAGTCCCACCAGTGACTGGATCAATGCATTGACTTTCACTAATAAGAACAACCCGCAACGAAATCAGTTGTTGCTCAGAAGCTTACTAGGAACGATCCCGGTATTGATCAATAAGAGTGGGGATAGTGGGGATGAGTTTAACCATACGAGTGACCAGAAATGGGATAAAACGAATGAAAAAGATGGGAATTTACCTGGGTTTGGGGAGGTGAATGGTGGTTTTATTATGTTTTTATCAAATCTTTATTTACTTTAA", "aa": "MTTITVSPFEQVKPSNGSNTFNPNSDDNKVTPSGGSPAKKTTYDFLPNSISPTSDWINALTFTNKNNPQRNQLLLRSLLGTIPVLINKSGDSGDEFNHTSDQKWDKTNEKDGNLPGFGEVNGGFIMFLSNLYLL", "aa_hexdigest": "c619ff6224ce24383a9bced809b9e70f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030D20171", "query_cov": 0.8507462686567164, "subject_cov": 0.9661016949152542, "identity": 0.833, "score": 178.0, "evalue": 9.5e-56, "valid": false, "product": "adhesin", "uniref50_id": "UniRef50_Q49206" }, "pscc": { "uniref50_id": "UniRef50_Q49206", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49206" ], "product": "Random genomic clone esh10, partial cds" }, "genes": [], "id": "KMADJIBOEG_427", "locus": "GCA000292445_00391" }, { "type": "cds", "sequence": "contig_1", "start": 214637, "stop": 214807, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13", "UniRef:UniRef90_UPI002FCE54A6" ], "nt": "GTGGTGAAAAAATATAAGTTAGATCTACCAAATGTTACCAATGAGATGAACCAAGTGTTGCAAGAATTGATCCTAGAACAACCTTTGACTAAGTATACCTTAAATTCGAGTTTGGCTAAACAAAAGGGCAAAAGCCAGATAGTGGTGCATCTTGGAAAATCAAATAAGTGA", "aa": "MVKKYKLDLPNVTNEMNQVLQELILEQPLTKYTLNSSLAKQKGKSQIVVHLGKSNK", "aa_hexdigest": "d55fcf9e021d4bb23b9da7fcb8951d09", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 214487, "stop": 214872, "observations": { "deletion": [ 214805 ], "mutation": [ 214631 ], "stop": [ 214631 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FCE54A6", "query_cov": 0.9285714285714286, "subject_cov": 0.41935483870967744, "identity": 0.885, "score": 94.0, "evalue": 1.48e-23, "gene_start": 1, "gene_end": 52, "reference_start": 47, "reference_end": 98, "reference_sequence": "MLFKSTQVKLTYSSSGSNNISFENNANKPSYVVEFTNSTTVGIKWSVVKKYQLDVPNVTNEMNQVLQELILEQPLTKYTLNGSLAKQKGKSQVAVHLGGQASQWHSTRHSQSKQQSQPQGFNRI" }, "paralog": false, "identity": 0.703125, "score": 157.0, "evalue": 3.4e-51, "description": "Internal stop codon at 214631. Frameshift due to deletion around 214805. Nonsense mutation around 214631.", "psc": { "uniref90_id": "UniRef90_UPI002FCE54A6", "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FCE54A6", "UniRef:UniRef50_UPI00027B3D13" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6382.452500000001, "isoelectric_point": 9.757306098937985 }, "id": "KMADJIBOEG_428", "locus": "GCA000292445_00392" }, { "type": "cds", "sequence": "contig_1", "start": 214814, "stop": 214915, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGCAACCAACACAACCTAAACAACAATCCCTCTCCCAATGCTTCAACCGGATTTAAATTAGATAAAGGCAACGCATATAGAAAACTAAGTGAGTCCTGA", "aa": "MRNQHNLNNNPSPNASTGFKLDKGNAYRKLSES", "aa_hexdigest": "0fe68a92074324d3b26738fce2aeaf9b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3704.009400000001, "isoelectric_point": 9.992035484313966 }, "id": "KMADJIBOEG_429", "locus": "GCA000292445_00393" }, { "type": "cds", "sequence": "contig_1", "start": 215123, "stop": 215545, "strand": "+", "frame": 2, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49384", "UniRef:UniRef90_UPI0029622FB5" ], "nt": "ATGTGGTTACCCTCCTTCCTCTACTTTCAACCCAACAAGGTGAAAACAAATGCTTACCAAACCACTAACACCTACAACAAGTTAATTGAACCGGAAAGTGCAACAAGTGCAGCAACTAACATGACCAACTTGTTAAAGCTGTTGTCTAGTAAAAACATCAAACAAAAGTTGGGGAAGGGAACAGCTTCTTCGCAGGGAAGTTTCAGTGTCCAAGACACCTTTTCGTTTGTTGTTCCTTATTCATCGAATCACACAAATAATGGAAACACTTCAGGAACCATTAAAACTGCTTATCCGGTGAAAAACACAGAGAAAGCAACAGTAATGATCAATTCCTTGATCAACGCTACGCCGTTGAATAGTTATGGGGATTTAAATAATCACACCAAGTTAATTTACCCAAATTATTTACTTATTTATTAA", "aa": "MWLPSFLYFQPNKVKTNAYQTTNTYNKLIEPESATSAATNMTNLLKLLSSKNIKQKLGKGTASSQGSFSVQDTFSFVVPYSSNHTNNGNTSGTIKTAYPVKNTEKATVMINSLINATPLNSYGDLNNHTKLIYPNYLLIY", "aa_hexdigest": "e10cfe8e88d6b81c4b73fb17303739f6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0029622FB5", "query_cov": 1.0, "subject_cov": 0.958904109589041, "identity": 1.0, "score": 268.0, "evalue": 9.26e-91, "valid": true, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_Q49384" }, "pscc": { "uniref50_id": "UniRef50_Q49384", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49384" ], "product": "Adhesin-like protein" }, "genes": [ "mgpC" ], "id": "KMADJIBOEG_430", "locus": "GCA000292445_00394" }, { "type": "cds", "sequence": "contig_1", "start": 215680, "stop": 215838, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGGGTTTTTAAAACCAAATTTCTCTCTCGGTGCTTTGTTTTTAACTTTAAGCCCCATAGCCACTGCATGCATTGCTGAAAAACCAGTTAACAACCGCTTTAACTTTAATAGCGAGCAATTAGCTAGGCTAAGAAAAGCAAGGGTTAACCACTGA", "aa": "MKGFLKPNFSLGALFLTLSPIATACIAEKPVNNRFNFNSEQLARLRKARVNH", "aa_hexdigest": "ba96a79688dc3cbf84dc2154b7a16318", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5845.803, "isoelectric_point": 11.006509971618652 }, "id": "KMADJIBOEG_431", "locus": "GCA000292445_00395" }, { "type": "cds", "sequence": "contig_1", "start": 215854, "stop": 216030, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAGTTAGCTTTGCAAATAACCACCAAAAACCGATCCGTATCTATGCCATTGATACCCCTGAAAAAGCAGTTTTATCTATACAACGCAAATCAGAGATAGAACTTAAAGAAGCTAATAAAGCAACTGAGTTTGCCAAAAGCTTAATTCCCATTGGTAGTGAGGTGTGGATCTGA", "aa": "MEVSFANNHQKPIRIYAIDTPEKAVLSIQRKSEIELKEANKATEFAKSLIPIGSEVWI", "aa_hexdigest": "5b63f637902c862a17c023ab7011c0ee", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6583.523299999997, "isoelectric_point": 6.557253074645996 }, "id": "KMADJIBOEG_432", "locus": "GCA000292445_00396" }, { "type": "cds", "sequence": "contig_1", "start": 216070, "stop": 216342, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B418B8", "UniRef:UniRef90_UPI0001B418B8" ], "nt": "GTGTTTTTCAAAACCAATCCATTGCAACTGCACTTTGAATCGTTTGCAGTTGAAATGGTAGCAAATGGTCATGCTTTACCTATTGCTGGTAATGACTTTGATCTTGTTTTTAGTGATTTGGACCCATTTAATCCCCTAAAAATAGTAGGGATTGAACTAGCTAATGGTTTAAACAATGCTTTTAACAACAGAAAAAACATCTTTAGTTATTTAGAAAACAGCTTTCAATCAATAACAATGGTCTATCAACAACGCGGTGTTGACCAAGGTTGA", "aa": "MFFKTNPLQLHFESFAVEMVANGHALPIAGNDFDLVFSDLDPFNPLKIVGIELANGLNNAFNNRKNIFSYLENSFQSITMVYQQRGVDQG", "aa_hexdigest": "f73ba05685c2646e5aa7330c532707fb", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 216019, "stop": 216432, "observations": { "mutation": [ 216028, 216340 ], "stop": [ 216028, 216340 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001B418B8", "query_cov": 0.9888888888888889, "subject_cov": 0.6496350364963503, "identity": 0.978, "score": 176.0, "evalue": 2.4e-55, "gene_start": 1, "gene_end": 89, "reference_start": 18, "reference_end": 106, "reference_sequence": "MWIWPLNSYSYDREVAAVFFKTNPLQLHFESFAVEMVANGHALPIAGNDFDFVFSDLDPFNPLKIVGIELANGLNNAFNNRKNIFSYLENSFQSITMVYQQRGVDQSWTRYLAPSNDFSSTKLGLGLTIYELKLNNG" }, "paralog": false, "identity": 0.9635036496350365, "score": 266.0, "evalue": 1.53e-93, "description": "Internal stop codon at 216028, 216340. Nonsense mutation around 216028, 216340.", "psc": { "uniref90_id": "UniRef90_UPI0001B418B8", "uniref50_id": "UniRef50_UPI0001B418B8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001B418B8", "UniRef:UniRef50_UPI0001B418B8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001B418B8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B418B8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10142.344899999996, "isoelectric_point": 4.7775674819946286 }, "id": "KMADJIBOEG_433", "locus": "GCA000292445_00397" }, { "type": "cds", "sequence": "contig_1", "start": 216425, "stop": 216754, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33", "PFAM:PF03215.21" ], "nt": "ATGGCTAACAATAAGAGTGCAATTGAGTTGAAAAACATCGTTATTGATTTTGGTGAATCAGTTGCGATTGACAACATTAACCTTAGTGTTGAAAAACACCAACTAGTTAGCTTACTTGGTCCTAGTGGTTGTGGTAAAACCACTACACTTGCAGTTATTGCAGGACTTATTAAACCAACTAGTGGTCAGGTGTTATTTAATGGTTATGATGTCACCAAAAAACCACCCCAAGAACGTAAACTAGGGCTAGTTTTTCAAAACTATGCACTTTATCCGCACATGAATGTGTTTGAAAACATTGTTTTCCCCCTCTACAGTGATAACTCGTGA", "aa": "MANNKSAIELKNIVIDFGESVAIDNINLSVEKHQLVSLLGPSGCGKTTTLAVIAGLIKPTSGQVLFNGYDVTKKPPQERKLGLVFQNYALYPHMNVFENIVFPLYSDNS", "aa_hexdigest": "9abe1f7efbce8c681406a7ceb5446654", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034E961FD", "query_cov": 0.8440366972477065, "subject_cov": 0.8679245283018868, "identity": 0.5329999999999999, "score": 113.0, "evalue": 1.32e-30, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_A0A3B9G3L7" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 78, "aa_cov": 0.6972477064220184, "hmm_cov": 1.0, "evalue": 3.0160109601035743e-21, "score": 71.61136627197266, "start": 24, "stop": 99 }, { "id": "PF03215.21", "name": "Rad17", "length": 79, "aa_cov": 0.7155963302752294, "hmm_cov": 0.9873417721518988, "evalue": 4.676431214107345e-08, "score": 28.320594787597656, "start": 23, "stop": 100 } ], "seq_stats": { "molecular_weight": 11909.585999999988, "isoelectric_point": 6.710377311706543 }, "id": "KMADJIBOEG_434", "locus": "GCA000292445_00398" }, { "type": "cds", "sequence": "contig_1", "start": 216767, "stop": 217060, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAAAAAACAGTGTTGCAAACCATGAGATTAACTGTTTGTTACTTACTAGCAACGGTGCATCAGTTCAAGAGATTGATCAGCTCAATAAGTTATTTCATGATAGTATTGAAAAACCCAAACAGATCCAATACCAAATTAATGACCTTAATGTTAGTGTTTTTAAAAACTTAAATGAACTAACTGCAAACCTTAAGTTAATACCAAGTAAACACCAGTTTGCTATTACCAATCTCAACAAACAAACTCTAAAACAGATTAATGAACTGGAAGCTGAGTTTAAAACAAAGTGA", "aa": "MEKNSVANHEINCLLLTSNGASVQEIDQLNKLFHDSIEKPKQIQYQINDLNVSVFKNLNELTANLKLIPSKHQFAITNLNKQTLKQINELEAEFKTK", "aa_hexdigest": "df1742a48338dccf1a178445687dc124", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11119.565499999986, "isoelectric_point": 6.824282646179199 }, "id": "KMADJIBOEG_435", "locus": "GCA000292445_00399" }, { "type": "cds", "sequence": "contig_1", "start": 217181, "stop": 217885, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "GTGGAACTAAAACAAACCCTTGTTGAAAACCTCAAGTTAGTTAAAAAAGCGATTAGTGAACAAACTAAGTCAATTAAACAGAGCAGTGATTACACTAAGTTAAAGCAACTAAAACGGTTAATTAAAGTTGAACCTAACCAACTCAAAAAACAATATAAGGTTTTTCTCAATCAGTTAATTAAAAACTATTCACTTAAAACTGATAAGTTAACTGATACTCAACTTAATGAAATTGAACAGATTAAAACCAGAATTGTTTCAATAAAACAGTTTATCAACAAAACTGCACTTGAAGTTGCTAACAAACTAGCGATTACCAAGATTTTAACCAAACGCCCTGATAAGATTTCTGGTGGACAACAACAACGCGTAGCAATTGCTAGAGCAATTGTCAGAAGACCTAAACTATTGTTAATGGATGAACCACTCTCTAACTTAGATGCAAAGCTAAGGGTACAGACAAGACAGTGGATCAGACAGTTTCAACAGGAGTTACAAATTACCACTGTTTTTGTCACCCATGACCAGGAAGAAGCGATGAGTATTAGTGATGTCATTGTTTGTATGTCAACTGGAAAAGTGCAGCAAATCGGCACTCCCAGTGAACTTTATTTAAAACCTGCTAATGAGTTTGTTGCGCGCTTTTTAGGCACCCCTGAGATGAACATCATTGAATGTAGTGTCAAAAACAACCAGTTGTTTTGA", "aa": "MELKQTLVENLKLVKKAISEQTKSIKQSSDYTKLKQLKRLIKVEPNQLKKQYKVFLNQLIKNYSLKTDKLTDTQLNEIEQIKTRIVSIKQFINKTALEVANKLAITKILTKRPDKISGGQQQRVAIARAIVRRPKLLLMDEPLSNLDAKLRVQTRQWIRQFQQELQITTVFVTHDQEEAMSISDVIVCMSTGKVQQIGTPSELYLKPANEFVARFLGTPEMNIIECSVKNNQLF", "aa_hexdigest": "4d3a99cf57672cc4dcf24c89f9d7382b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 118, "aa_cov": 0.47863247863247865, "hmm_cov": 0.7203389830508474, "evalue": 6.252590202179728e-14, "score": 47.915016174316406, "start": 32, "stop": 143 } ], "seq_stats": { "molecular_weight": 26982.370600000006, "isoelectric_point": 9.8450475692749 }, "id": "KMADJIBOEG_436", "locus": "GCA000292445_00400" }, { "type": "cds", "sequence": "contig_1", "start": 217964, "stop": 218182, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTCACTACTAACAAAAGTAGTTTGCAAGCTAAACTAATTAACATTGAAAACTTAGGTAAACACTTAGTTGCTACCATTAGTTTGTTTGATACCACCTTATCAATGCGCTTAGAATTGAATAGCCACTTAAAAGTAGGTGATAGTTTAAATTTCATTATTAAAGCTAACAACCTCCATTTTTTTGATATTGATACAAAACAACGGATTGAGATTTAA", "aa": "MVTTNKSSLQAKLINIENLGKHLVATISLFDTTLSMRLELNSHLKVGDSLNFIIKANNLHFFDIDTKQRIEI", "aa_hexdigest": "e837cd6c9e16c876478aabd4345a93c9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8168.404699999995, "isoelectric_point": 8.158812522888184 }, "id": "KMADJIBOEG_437", "locus": "GCA000292445_00401" }, { "type": "cds", "sequence": "contig_1", "start": 218275, "stop": 218559, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTGTTCCTCCCTGCGCTTTTAACAACAATTTTGTTTACCATTATCCCCTTCTTTTTAAGCTTACAGAAGGGTTTTAGTGCTAACAGTGATCTGTATGATCTCTCCTCCCAATCCTTTAGTTTACGAACCTTTCAGGATCTGTTTAGTGAATCTAACTTTGTGTTGGGCTTACGTAACAGTTTTCTCTATTCACTAATCTCTTTACCCTTTAGCATTATTATTGCTATTGTCATTGCCAGTGCCATAGTATTTGTGTACAAAAAGCTGTTAAGAGGGTTTTGA", "aa": "MLFLPALLTTILFTIIPFFLSLQKGFSANSDLYDLSSQSFSLRTFQDLFSESNFVLGLRNSFLYSLISLPFSIIIAIVIASAIVFVYKKLLRGF", "aa_hexdigest": "883e1a9ad8066f89cfaaace6f58f86ba", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10612.459499999988, "isoelectric_point": 9.30054759979248 }, "id": "KMADJIBOEG_438", "locus": "GCA000292445_00402" }, { "type": "cds", "sequence": "contig_1", "start": 218566, "stop": 219129, "strand": "+", "frame": 1, "gene": "ugpA", "product": "putative ABC transporter permease protein MG188", "db_xrefs": [ "COG:COG1175", "COG:G", "GO:0005886", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P75263", "UniRef:UniRef90_P47434" ], "nt": "GTGTTTTTTTTACCCTATGTAACTTCAGGGGTGGCAATCTCGATTGCCTTTGTATATATCTTTGATTCTGCCTCTGGTATTTTAAACACGGTGTTTAATGTCAACACCAAGTGGCTTGATTCAGGTTCACGTGATACATTTAATGCCTTGTGGGCTATCTTAATCTTTGGGGTGTGGAAAAACTTGGCATTTAATGTGTTGATTATCTCAACAGCAATGTTAAGTGTTAATCCCCAACTTTACAAGGTAGCAAGTTTAGATAGTGCCAATCCTGTAAGACAGTTCTTTAAGATCACTCTTCCCTCCATCCGTCCTACTTTAATCTTTCTTACTACCCTTTTAATCTTAGGGGGGATGCAAGTCTTTCCGCTGGCTTTGTTTGAAAACAAACCTGAAGAAGCGGTTGCTAATGGGGGGAATAGTATCTTGCTTTACATCTTTCAACAGATCCAAAGTGGCAACACTAACTTAGCAGGTGCTGCTACTTTAGTGTTGCTTGTGTTGGGGGTTTGTTATGGGTTAGTGTTACGCAATGGTTTTTATCTGATTGAATGGTTGCAGTGA", "aa": "MFFLPYVTSGVAISIAFVYIFDSASGILNTVFNVNTKWLDSGSRDTFNALWAILIFGVWKNLAFNVLIISTAMLSVNPQLYKVASLDSANPVRQFFKITLPSIRPTLIFLTTLLILGGMQVFPLALFENKPEEAVANGGNSILLYIFQQIQSGNTNLAGAATLVLLVLGVCYGLVLRNGFYLIEWLQ", "aa_hexdigest": "cee12aca3c9346b21a35533ff76874af", "start_type": "GTG", "rbs_motif": null, "genes": [ "ugpA" ], "truncated": "both", "pseudogene": { "start": 218266, "stop": 219174, "observations": { "mutation": [ 218557, 219127 ], "stop": [ 218557, 219127 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47434", "query_cov": 1.0, "subject_cov": 0.5683890577507599, "identity": 0.9890000000000001, "score": 356.0, "evalue": 6.32e-122, "gene_start": 1, "gene_end": 187, "reference_start": 128, "reference_end": 314, "reference_sequence": "MFKWLLKHHNQPHSLQLGLLDQPLPFWKPFLLFLPALLTTILFTIIPFFLSLQKGFSANSDLYDLSSQSFSLRTFQDLFSESNFVLGLRNSFLYSLISLPFSIIIAIVIASAIVFVYKKLLRGFWQTVFFLPYVTSGVAISIAFVYIFDSASGILNTVFNVNTKWLDSGSRDTFNALWAILIFGVWKNLAFNVLIISTAMLSVNPQLYKVASLDSANPVRQFFKITLPSIRPTLIFLTTLLILGGMQVFPLALFENKPEEAVANGGNSILLYIFQQIQSGNTNLAGAATLVLFVLGVCYGLVLRNGFYLIEWLQWKIKQLYVQKQLTLY" }, "paralog": false, "identity": 0.9900662251655629, "score": 575.0, "evalue": 7.74e-211, "description": "Internal stop codon at 218557, 219127. Nonsense mutation around 218557, 219127.", "psc": { "uniref90_id": "UniRef90_P47434", "gene": "UgpA", "product": "Probable ABC transporter permease protein MG188", "uniref50_id": "UniRef50_P75263", "cog_id": "COG1175", "cog_category": "G", "go_ids": [ "GO:0005886", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47434", "UniRef:UniRef50_P75263", "COG:COG1175", "COG:G", "GO:0005886", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P75263", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75263" ], "product": "Probable ABC transporter permease protein MG188 homolog" } }, "id": "KMADJIBOEG_439", "locus": "GCA000292445_00403" }, { "type": "cds", "sequence": "contig_1", "start": 219674, "stop": 219829, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCCTTTGTTAGCAGTGTTTTTAGTGGGTTTATGTACCGTACTAGCTTTGAAGCCATTCCATCTCAATTAAAAGAGTCAGCACTCATTGATGGGTGTAATGGCTTTAACTACTTTTTGAAGATTGCTTTACCAATGGTGAAATCTACCAGTTGA", "aa": "MPFVSSVFSGFMYRTSFEAIPSQLKESALIDGCNGFNYFLKIALPMVKSTS", "aa_hexdigest": "085b3f2dbe63731aa5df8fccf6c2c25d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5658.5434, "isoelectric_point": 7.899262809753418 }, "id": "KMADJIBOEG_440", "locus": "GCA000292445_00404" }, { "type": "cds", "sequence": "contig_1", "start": 219890, "stop": 220102, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGCAACAGGGTGGATTTAAACATTAACTTGTGGGTGTTACAACAAGGGATCTTGGATGCTAACAGTAGTGATGAACAGATCAGAACGCTGTTAAATCTCAAGATGAGTGCAGCGATTCTAGCCATCCTTCCGATGTTTATTATCTACTTTTTGTTCCATAAAAGGATTATGAATGCCATTAAAAACAGAGCCAACACCATTAAGGGTTAA", "aa": "MGNRVDLNINLWVLQQGILDANSSDEQIRTLLNLKMSAAILAILPMFIIYFLFHKRIMNAIKNRANTIKG", "aa_hexdigest": "8baf63335e561f46c561ee1e85c6384c", "start_type": "TTG", "rbs_motif": "ATTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7999.449899999991, "isoelectric_point": 10.15604305267334 }, "id": "KMADJIBOEG_441", "locus": "GCA000292445_00405" }, { "type": "cds", "sequence": "contig_1", "start": 220104, "stop": 220262, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_158309353.1", "SO:0001217", "UniParc:UPI0002D36BE5", "UniRef:UniRef100_UPI0002D36BE5", "UniRef:UniRef50_UPI0002D36BE5", "UniRef:UniRef90_UPI0002D36BE5" ], "nt": "ATGCAAAAGTTTAAACAACTGGTTGGTGCAATGCACAGATGGGTAAAACTAGCACTATTAGTAATCATTGTGTTATTGGGGATTATCTTTTGTCTGTTTGCCATCTATGGCATTGCGCAAGTGATCATTACCATTATCAATGAAGGGGCACTTTTATAA", "aa": "MQKFKQLVGAMHRWVKLALLVIIVLLGIIFCLFAIYGIAQVIITIINEGALL", "aa_hexdigest": "8f883cd9601e4502159aa6088bc6e785", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002D36BE5", "ncbi_nrp_id": "WP_158309353.1", "uniref100_id": "UniRef100_UPI0002D36BE5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002D36BE5", "RefSeq:WP_158309353.1", "UniRef:UniRef100_UPI0002D36BE5" ] }, "ips": { "uniref100_id": "UniRef100_UPI0002D36BE5", "uniref90_id": "UniRef90_UPI0002D36BE5" }, "psc": { "uniref90_id": "UniRef90_UPI0002D36BE5", "uniref50_id": "UniRef50_UPI0002D36BE5" }, "pscc": { "uniref50_id": "UniRef50_UPI0002D36BE5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D36BE5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5810.248799999998, "isoelectric_point": 9.626564216613769 }, "id": "KMADJIBOEG_442", "locus": "GCA000292445_00406" }, { "type": "cds", "sequence": "contig_1", "start": 220271, "stop": 221038, "strand": "+", "frame": 2, "gene": "nrnA", "product": "Bifunctional oligoribonuclease and PAP phosphatase NrnA", "db_xrefs": [ "COG:COG0618", "COG:FT", "EC:3.1.-.-", "GO:0003676", "GO:0004527", "GO:0008441", "KEGG:K06881", "SO:0001217", "UniRef:UniRef50_P75144", "UniRef:UniRef90_P22746" ], "nt": "ATGAAAAAAGGATCAATAACTGAAGCAATTAATGCCATTAAACAATTTGATAAGATTGTTATCTTTCACCATGTGCGCCCTGACGGGGATTGTTTAGGAGCACAACAAGGTTTGTTTCACCTCATTAAAGCTAACTTTAAAGATAAGGAGGTGAAGTGTGTTGGTAATAACAACAACCTGTTTAGCTTTATCAACATGACATTTACCAACCAAATTGATGAGAGCTTTTTAAAAGAAGCACTTGCGATTGTGGTGGATGCTAATTACAAAAACAGGATTGAATTGAGAGAACTGTTAGATAAAAACCTGTTTAAAGCAGTGTTAAGGATTGATCACCATCCCAATGAAGATGATCTAAACACTAGCTTTAACTTTGTTGAAGAAAGCTATGTAGCTTGTTGTGAGCAGATAGTGGAGATGGCCACAGTGGCGAAGTGGACCATACCACCAGTGGCTGCTACTTTACTATATATAGGTATCTATACGGATAGTAATAGATTTCTATATAGTAATACATCATATAGAACACTATACTTAGCAGCAATACTATATAAAGCTAAAGCTGATATAAGGATAGTACATGATCATTTAAACCATACTAGTTTAGCAGATCTTAAGTTTAAAAAGTATGTTTATAACCACTTTAAAACCCAAGGACAAGTGATCTATTTTATCTGTACTAAAAAGATCCAAAAGAGACTAAGAATGACTGCAGATCAATGTGCTAGAGTTAACTTGTTAAGTAACATAGCAGATTACAAGATCTGA", "aa": "MKKGSITEAINAIKQFDKIVIFHHVRPDGDCLGAQQGLFHLIKANFKDKEVKCVGNNNNLFSFINMTFTNQIDESFLKEALAIVVDANYKNRIELRELLDKNLFKAVLRIDHHPNEDDLNTSFNFVEESYVACCEQIVEMATVAKWTIPPVAATLLYIGIYTDSNRFLYSNTSYRTLYLAAILYKAKADIRIVHDHLNHTSLADLKFKKYVYNHFKTQGQVIYFICTKKIQKRLRMTADQCARVNLLSNIADYKI", "aa_hexdigest": "88b531f7a26b98e01ab4777f5ecb5743", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P22746", "query_cov": 1.0, "subject_cov": 0.8018867924528302, "identity": 0.996, "score": 510.0, "evalue": 6e-182, "valid": true, "gene": "nrnA", "product": "Bifunctional oligoribonuclease and PAP phosphatase NrnA", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75144", "cog_id": "COG0618", "cog_category": "FT", "kegg_orthology_id": "K06881", "go_ids": [ "GO:0008441", "GO:0004527", "GO:0003676" ] }, "pscc": { "uniref50_id": "UniRef50_P75144", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75144" ], "product": "Bifunctional oligoribonuclease and PAP phosphatase NrnA" }, "genes": [ "nrnA" ], "id": "KMADJIBOEG_443", "locus": "GCA000292445_00407" }, { "type": "cds", "sequence": "contig_1", "start": 221675, "stop": 222142, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "ATGGAAAACCCCTCAACGGTGCAAAGGGGTTTAAATGGCGAGCCTATCTTTGATCCTTTTAAAGGCTTTGGTTTAACTGGTAATGCCCCTACTGATTGGAATGAGATCAAAGGTAAAGTTCCAGTAGAAGTAGTTCAATCCCCCCATTCCCCCAACCTCTATTTTGTGTTACTAGTGCCTAAGGTGGTAGTGGAGTACCACCAACTTGATAAGGTTGTCAAAGAGAGTTTGGAAGTGGAAGCAACTTCCAACTTTGATCCAACTAAAAGGTTGCAAAAAGAGAGTCCACAAAAAGATTCAAGTAAACAAGCAGAGAAACTCACCGAAACAACTTCATCGATGAGTAGTGGTGGGGCTACATCCACTCGAGCCAAGGCCCTCAAGGTTGAGGTGGAAAGGGGGAGTCAAAGTGATTCACTTTTAAAAAACGACTTTGCTAAAAAGCCACTGAAACATAAAGAACAATAG", "aa": "MENPSTVQRGLNGEPIFDPFKGFGLTGNAPTDWNEIKGKVPVEVVQSPHSPNLYFVLLVPKVVVEYHQLDKVVKESLEVEATSNFDPTKRLQKESPQKDSSKQAEKLTETTSSMSSGGATSTRAKALKVEVERGSQSDSLLKNDFAKKPLKHKEQ", "aa_hexdigest": "ec5507a26fedf8555465d6dbfa47a61e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 86, "aa_cov": 0.5548387096774193, "hmm_cov": 0.7325581395348837, "evalue": 8.81325425703073e-19, "score": 63.03961181640625, "start": 7, "stop": 92 } ], "seq_stats": { "molecular_weight": 17124.107099999994, "isoelectric_point": 7.893460655212403 }, "id": "KMADJIBOEG_444", "locus": "GCA000292445_00408" }, { "type": "cds", "sequence": "contig_1", "start": 222193, "stop": 222345, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "TTGTTGAAAACAGATGAGATAGCGAAGGAGAAGGGGATGGGGGCGACGGTGGTTAGTTTCTATGATGCACCCTACAGTGAAAACCATACTGCCTTTGGACTTGTTGATCACATCGATCCTAAAAAGATGGTTGAAAACTACCCACCAAGTTGA", "aa": "MLKTDEIAKEKGMGATVVSFYDAPYSENHTAFGLVDHIDPKKMVENYPPS", "aa_hexdigest": "9ad10684e7e2b3dc8385c15ffdd149aa", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 49, "aa_cov": 0.94, "hmm_cov": 1.0, "evalue": 1.5405364358760514e-11, "score": 39.27266311645508, "start": 4, "stop": 50 } ], "seq_stats": { "molecular_weight": 5573.245700000001, "isoelectric_point": 5.0413003921508786 }, "id": "KMADJIBOEG_445", "locus": "GCA000292445_00409" }, { "type": "cds", "sequence": "contig_1", "start": 222400, "stop": 222738, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "TTGTTACAAACAACAGGGTTCTTTAACCCAAGAAGACACCCAGAGTGGTTTGATGAAGGACAAGCTAAGGCAGATAACACTAGCCCTGGCTTTAAGGTAGGGGAAAGTGATCACAAAAAAGACGGCTTTAAAGACTCTTCTTCTTCTTCTTCTCCAATAGCTTTACCATTTGAAGCATACTTTGCTAATATTGGTAACATGGTTGCTATTGGCAACTCGGTATTTATCTTTGGTGGTAATGGTCATGCTACTAAGATGTTTACCACCAATCCCTTAAGTATTGGGGTATTTAGGATTAAATACACTGATAACTTTAGTAAGTCATCAGTAACTGGTTGA", "aa": "MLQTTGFFNPRRHPEWFDEGQAKADNTSPGFKVGESDHKKDGFKDSSSSSSPIALPFEAYFANIGNMVAIGNSVFIFGGNGHATKMFTTNPLSIGVFRIKYTDNFSKSSVTG", "aa_hexdigest": "b32222f093d14ec37a4333d1361c915c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 109, "aa_cov": 0.9732142857142857, "hmm_cov": 0.7798165137614679, "evalue": 3.4388847784781427e-34, "score": 113.60453033447266, "start": 4, "stop": 112 } ], "seq_stats": { "molecular_weight": 12191.419799999987, "isoelectric_point": 8.04483242034912 }, "id": "KMADJIBOEG_446", "locus": "GCA000292445_00410" }, { "type": "cds", "sequence": "contig_1", "start": 222748, "stop": 223020, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "GTGTTATTTGGAGGATTAATTAATCCCCAAACCAATGGCTTGAAGGATCTTCCCCTTGGTACCAACAGGTGGTTTGAATATGTACCAAGAATGGCAGTTAGTGGGGTAAAATGGGTTGGTAATCAACTAGTGTTAGCAGGAACACTAACAATGGGTGATACAGCTACTGTACCTAGGTTAAAGTATGACCAACTAGAAAAACACCTAAACCTAGTTGCTCAAGGCCAGGGACTATTGAGAGAAGACTTGCAGATCTTCACTCCCTATGGGTGA", "aa": "MLFGGLINPQTNGLKDLPLGTNRWFEYVPRMAVSGVKWVGNQLVLAGTLTMGDTATVPRLKYDQLEKHLNLVAQGQGLLREDLQIFTPYG", "aa_hexdigest": "906032a5b7f3406e7d53e54cdf52c5f9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 36, "aa_cov": 0.3888888888888889, "hmm_cov": 1.0, "evalue": 2.8692617017070336e-10, "score": 35.104736328125, "start": 1, "stop": 35 } ], "seq_stats": { "molecular_weight": 9986.4679, "isoelectric_point": 8.085705375671388 }, "id": "KMADJIBOEG_447", "locus": "GCA000292445_00411" }, { "type": "cds", "sequence": "contig_1", "start": 223066, "stop": 223221, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCAGTAAATTTGGTCCCCATTACTTCTTAAATAACCCTGATATCCAGGACAATGTTAATAATTATACGGTTGAAGTATTAATCAATACCTATACAAACACTGATAAGTTAAAACACGTTTATCCTTATCGATACAGTGGTTTGTATGCTTGA", "aa": "MGSKFGPHYFLNNPDIQDNVNNYTVEVLINTYTNTDKLKHVYPYRYSGLYA", "aa_hexdigest": "4d1901d6aa0bab2f153bd51218922c66", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5989.593300000002, "isoelectric_point": 6.695087623596191 }, "id": "KMADJIBOEG_448", "locus": "GCA000292445_00412" }, { "type": "cds", "sequence": "contig_1", "start": 223306, "stop": 223449, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGCTGCTATCTTAAATGAAGATCTGTTAACAGGGCTAAGTGATAAGATTGTCTATGGTAAGGAGAATGAGTTTGCTGAAAATGAAGCAGATAGGTTTAACCAACTTTTAAGTTTAAATCCTAGTCCTAACACTAACTGA", "aa": "MFAAILNEDLLTGLSDKIVYGKENEFAENEADRFNQLLSLNPSPNTN", "aa_hexdigest": "8ebb7387a7c032510490e91c76d01592", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5257.749500000001, "isoelectric_point": 4.1193151473999015 }, "id": "KMADJIBOEG_449", "locus": "GCA000292445_00413" }, { "type": "cds", "sequence": "contig_1", "start": 223777, "stop": 224238, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAGCCTAGTAATAGTAGTACCCCCTTTGATCCCAATTCTTATGAAAACCAAGTCACACCATCAGGTAGCTCCCCCAAAACAACCACCTATCCTTCTTTACCAAACTCCATCAGTCCCACCAGTGACTGGATCAACGCATTAACCTTCACTAATAAGAACAATCCACAACGCAATCAGTTGTTGTTAAGGGGGTTGTTAGGAACTATTCCGGTATTGATCAATAAGAGTGGGGATAGTAATGATCAATTTAACAAGGATAGTGAGCAGAAGTGGGATAAAACTGAGACAAATGAGGGCAACCTCCCAGGGTTTGGGGAGGTGAATGGGTTGTATAATGCCGCATTACTCCATACCTATGGTTTTTTTGGCACCAATACCAACTCTACTGATCCTAAGATAGGCTTTAAAGCTGATAGTAGTAGTAGTAGTAGTACACTAGTAGGTAGTGGGTTAAACTGA", "aa": "MKPSNSSTPFDPNSYENQVTPSGSSPKTTTYPSLPNSISPTSDWINALTFTNKNNPQRNQLLLRGLLGTIPVLINKSGDSNDQFNKDSEQKWDKTETNEGNLPGFGEVNGLYNAALLHTYGFFGTNTNSTDPKIGFKADSSSSSSTLVGSGLN", "aa_hexdigest": "c53d48cebfd6a74a8cc4e3fc95fe726e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16453.708799999982, "isoelectric_point": 5.251320457458495 }, "id": "KMADJIBOEG_450", "locus": "GCA000292445_00414" }, { "type": "cds", "sequence": "contig_1", "start": 224894, "stop": 225568, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGGTAAACTAACTGGCGTAGTATCTACTCCTCTTGTTAACCTTATCAATGGTCAGGGAGCAACTAGTGATAGTGATACTGAAAAAATTAGCTTTAAATCTGGTAACCAGATTGACTTTAATAGGTTATTTACCTTACCAGTAACTGAACTATTTGATCCTAACACGATGTTTGTCTATAACCAGTATGTACCACTATTGGTTAACTTACCTAGTGACTTTGATCAAGCTTCAATCCGCTTAAAGGTAATTAGTTACTCAGTAGAAAACCAAACCTTAGGAGTTAGATTAGAGTTCAAAGATCCTCAAACCCAACAGTTTATCCCGGTACTAAATGCATCAAGTACAGGTCCCCAAACTGTCTTTCAACCCTTTAACCAGTGGGCAGACTATGTCTTACCTTTGATTGTAACTGTTCCTATAGTTGTCATTATCCTTAGTGTTACTTTGGGATTAACGATTGGAATTCCAATGCACAGAAACAAAAAGGCATTACAAGCAGGGTTTGATCTTTCTAACAAAAAGGTTGATGTTTTGACCAAAGCAGTTGGTAGTGTCTTTAAAGAGATCATTAACAGAACAGGGATCTCTAACGCTCCTAAGAAGTTAAAACAAGCTACCCCAACCAAACCAACTCCTAAAACCCCACCAAAACCTCCAGTAAAACAATAA", "aa": "MDGKLTGVVSTPLVNLINGQGATSDSDTEKISFKSGNQIDFNRLFTLPVTELFDPNTMFVYNQYVPLLVNLPSDFDQASIRLKVISYSVENQTLGVRLEFKDPQTQQFIPVLNASSTGPQTVFQPFNQWADYVLPLIVTVPIVVIILSVTLGLTIGIPMHRNKKALQAGFDLSNKKVDVLTKAVGSVFKEIINRTGISNAPKKLKQATPTKPTPKTPPKPPVKQ", "aa_hexdigest": "41b28b70d9237da2101f55121d274bb1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24606.239800000018, "isoelectric_point": 9.572475242614743 }, "id": "KMADJIBOEG_451", "locus": "GCA000292445_00415" }, { "type": "cds", "sequence": "contig_1", "start": 225570, "stop": 225938, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACAATGAGAAAACAGATTTATAAAAAAGCATACTGGTTACTATTACCCTTTCTACCATTAGCACTAGCCAATACCTTCCTTGTCAAAGAGGATAGTAAGAATGTTACTGCTTACACTCCCTTCGCCACCCCTATCACCGATTCTAAAAGTGATCTGGTTAGTTTGGCACAACTTGATTCTTCCTATCAAATCTCCGACCAAACCATCCATAACACCAACTTGTTTGTGTTGTTCAAGTCTACTCAGGTAAAAGTTAAGTATGAGTCAAGTGGCAGTAACAACCAGATTAGTTTTGATTCAACTAACAACAAACCATCCTACATCGTTGAATTTACTAATTCTACCAACATTGGCATCAAGTGA", "aa": "MKTMRKQIYKKAYWLLLPFLPLALANTFLVKEDSKNVTAYTPFATPITDSKSDLVSLAQLDSSYQISDQTIHNTNLFVLFKSTQVKVKYESSGSNNQISFDSTNNKPSYIVEFTNSTNIGIK", "aa_hexdigest": "b533878e55a3f8cf1a6d8ab802a0a453", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13797.554499999986, "isoelectric_point": 9.301643562316894 }, "id": "KMADJIBOEG_452", "locus": "GCA000292445_00416" }, { "type": "cds", "sequence": "contig_1", "start": 225945, "stop": 226118, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13", "UniRef:UniRef90_UPI0030D43D23" ], "nt": "GTGAAAAAGTATCAGTTAGATGTACCGAATGTTTCTAGCAACATGAACCAAGTGTTGCAAAACCTCATCCTTGAACAACCTTTGACTAAGTATACCTTAAACAGTAGTTTGGCCAAACAAAAGGGCAAAAGCCAGATAGAGGTGCATCTGGGTAGTGGGCAAGCAAATCAGTGA", "aa": "MKKYQLDVPNVSSNMNQVLQNLILEQPLTKYTLNSSLAKQKGKSQIEVHLGSGQANQ", "aa_hexdigest": "170252cc2e98cd15e98b3ea8ae7e5b4c", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 225822, "stop": 226183, "observations": { "deletion": [ 226128 ], "mutation": [ 225936, 226116 ], "stop": [ 225936, 226116 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0030D43D23", "query_cov": 1.0, "subject_cov": 0.47107438016528924, "identity": 0.895, "score": 100.0, "evalue": 5.25e-26, "gene_start": 1, "gene_end": 57, "reference_start": 43, "reference_end": 99, "reference_sequence": "MKLTYSSSGSNTNTISFENNANKPSYVVEFTNSTNVGIKWRVVKKYQLDVPNVSSDMNQVLQELILEQPLTKYTLNSSLAKQKGKSKIEVHLGSGRATQWTSRTQLHWPKQQSQPKCINRI" }, "paralog": false, "identity": 0.743801652892562, "score": 163.0, "evalue": 1.23e-53, "description": "Internal stop codon at 225936, 226116. Frameshift due to deletion around 226128. Nonsense mutation around 225936, 226116.", "psc": { "uniref90_id": "UniRef90_UPI0030D43D23", "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0030D43D23", "UniRef:UniRef50_UPI00027B3D13" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6356.203100000001, "isoelectric_point": 9.523801612854005 }, "id": "KMADJIBOEG_453", "locus": "GCA000292445_00417" }, { "type": "cds", "sequence": "contig_1", "start": 226386, "stop": 226847, "strand": "+", "frame": 3, "gene": null, "product": "Cytadherence-related ORF6-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HQG9" ], "nt": "TTGGAAAGGATCGGCATCTTGTTTGATGATCAAACCCCAAGAAATGTTATCACGCAGCTCTATTATGCTTCAACTAGTAAGCTAGCAGTCACTAACAACCACATTGTCGTGATGGGTAACAGCTTTCTACCCAGCATGTGGTACTGGGTGGTGGATAGGAGTGCAACAACTGATTCATCATCAAAACCCACCTGGTTTGCTAACACCAATTTAGATTGGGGAGAAGACAAACAAAAACAATTTGTAGAGAATCAGTTGGGGTATAAGGAAACTACCAGTACCAATTCCCACAACTTCCATTCCAAATCTTTCACCCAACCTGCATATCTGATCAGTGGCATTGACAGTGTCAATGATCAAATCATCTTCAGTGGTTTTAAAGCGGGCAGTGTGGGGTATGATAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTACCAAAGACCAAGCACTTGCTTGA", "aa": "MERIGILFDDQTPRNVITQLYYASTSKLAVTNNHIVVMGNSFLPSMWYWVVDRSATTDSSSKPTWFANTNLDWGEDKQKQFVENQLGYKETTSTNSHNFHSKSFTQPAYLISGIDSVNDQIIFSGFKAGSVGYDSSSSSSSSSSSSTKDQALA", "aa_hexdigest": "43738ca2408653a3fc2b1faccb3e49e8", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003305ECA5", "query_cov": 1.0065359477124183, "subject_cov": 0.8555555555555555, "identity": 0.506, "score": 147.0, "evalue": 1.95e-42, "valid": false, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_A0AB33HQG9" }, "pscc": { "uniref50_id": "UniRef50_A0AB33HQG9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HQG9" ], "product": "Cytadherence-related protein ORF6 homolog" }, "genes": [], "id": "KMADJIBOEG_454", "locus": "GCA000292445_00418" }, { "type": "cds", "sequence": "contig_1", "start": 226893, "stop": 227276, "strand": "+", "frame": 3, "gene": "mgpC", "product": "MgpC protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8G0" ], "nt": "GTGACCAACGACACGGGATTAAATGGTCCGATCAATGGGAGTTTTTCAATCCAAGACACCTTCAGCTTTGTTGTTCCTTATTCAATGAATCATACAAATACGGGAACTAGTGGAACCATTAAAACGGCTTATCCAGTGAAAAAAGATGAAGCTTCAACAGTAATGATCAATTCTTTGATCAACGCTACGCCGTTGAATAGTTATGGGGATGAAGGGGTTGGGGTGTTTGATGCGTTAGGTTTAAACTATAACTTTAAATCTAACCAAGAACGTTTACCTTCCAGAACTGATCAGATCTTTGTTTATGGGATTGTCTCCCCTAATGAATTGCGAAGTGCTAAAAGTTCTGCTGATTCAACTGGTAGTGATACAAAGGTAAACTGA", "aa": "MTNDTGLNGPINGSFSIQDTFSFVVPYSMNHTNTGTSGTIKTAYPVKKDEASTVMINSLINATPLNSYGDEGVGVFDALGLNYNFKSNQERLPSRTDQIFVYGIVSPNELRSAKSSADSTGSDTKVN", "aa_hexdigest": "b8c0fbbda9c7be485973b3fd6c8dd553", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F305329", "query_cov": 0.8818897637795275, "subject_cov": 0.9824561403508771, "identity": 0.5710000000000001, "score": 129.0, "evalue": 1.37e-36, "valid": false, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_A0AB38W8G0" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8G0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8G0" ], "product": "MgpC protein" }, "genes": [ "mgpC" ], "id": "KMADJIBOEG_455", "locus": "GCA000292445_00419" }, { "type": "cds", "sequence": "contig_1", "start": 227481, "stop": 228725, "strand": "+", "frame": 3, "gene": null, "product": "adhesin P110", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0015882A22", "UniRef:UniRef90_UPI002FCE5F12" ], "nt": "ATGAGTGGTAAGAAAGCTAACTATGGAGCGGTTGTGTTAACACGGGGGGGTGTTGTTAGATTAAACTTTAACCCTGGTAATGATTCATTGCTTTCAACAACTGATAACAATATAGCACCTATCTCCTTCTCATTTACTCCGTTCACAGCTGCTGAGAGTGCAGTGGATCTCACTACCTTCAAAGAAGTTACCTATAACCAAGAATCAGGATTGTGGAGTTATGTCTTTGACAACTCCTTAAAACCAACCCATGATGGTAAACAAATTGCTGTCACTGATAACAAGGGCTTTAGTGTTATCACTGTCTCAAGAACTGGCATTGAACTAAACCAAGACCAAGCTACTACAACTCTTGATGTAGCACCTAGTGCACTAGCAGTGCAATCAGGGATCCAATCTACCATCCAAACCCTAACTGGAGTACTCCCACTTAGTGAGGAATTCAGTGCAGTTATTGCTAAAGATAGTGGTCAAAATAAGATTGATATCTATAAAAACAACAACGGGTTGTTTGAAATTGATACCCAACTAAGTAATAGTGTTGCCACCAACAACGGTGGGTTAGCACCTAGTTACACAGAAAACACGGTTGATGCATGGGGTAAAGTTGAGTTTGCTGATAACAGTGTATTGCAAGCAAGAAACCTAGTTGATAAAACTGTTGATGAGATCATCAATACCCCTGAAATCTTAAATTCCTTCTTTAGATTCACCCCTGCTTTTGAAGATCAAAAAGCTACCCTTGTTGCTACTAAGCAAAGTGATACGTCACTTAGTGTCTCACCAAGAATCCAGTTCTTAGATGGTAATTTCTATGATCTTAACTCCACCATCGCTGGGGTACCTTTAAACATTGGTTTCCCTTCAAGAGTGTTTGCTGGGTTTGCAGCACTCCCTGCATGGGTGATCCCTGTATCAGTAGGTTCTTCAGTTGGGATCTTGTTTATCTTGTTAGTCTTAGGACTTGGGATTGGGATCCCAATGTACAGGGTAAGAAAACTCCAAGATGCATCGTTTGTTAATGTCTTTAAAAAGGTTGATACACTCACAACTGCTGTCGGTAGTGTGTACAAAAAGATTATTACCCAAACTGGTGTGGTGAAAAAAGCACCTAGTGCATTGAAAGCTGCTAATCCTAGTGTTAAAAAACCTGCTGCTTTTTTAAAACCACCTGTTCAACCACCAAGTAAACCTGAAGGGGAACAAAAAGCTGTTGAAGTTAAGTCAGAAGAAACCAAAAGTTAG", "aa": "MSGKKANYGAVVLTRGGVVRLNFNPGNDSLLSTTDNNIAPISFSFTPFTAAESAVDLTTFKEVTYNQESGLWSYVFDNSLKPTHDGKQIAVTDNKGFSVITVSRTGIELNQDQATTTLDVAPSALAVQSGIQSTIQTLTGVLPLSEEFSAVIAKDSGQNKIDIYKNNNGLFEIDTQLSNSVATNNGGLAPSYTENTVDAWGKVEFADNSVLQARNLVDKTVDEIINTPEILNSFFRFTPAFEDQKATLVATKQSDTSLSVSPRIQFLDGNFYDLNSTIAGVPLNIGFPSRVFAGFAALPAWVIPVSVGSSVGILFILLVLGLGIGIPMYRVRKLQDASFVNVFKKVDTLTTAVGSVYKKIITQTGVVKKAPSALKAANPSVKKPAAFLKPPVQPPSKPEGEQKAVEVKSEETKS", "aa_hexdigest": "d64003c3c2fc37e9174b3bbae086e4b8", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 227181, "stop": 228725, "observations": { "mutation": [ 227274 ], "stop": [ 227274 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FCE5F12", "query_cov": 1.0, "subject_cov": 0.5806451612903226, "identity": 0.966, "score": 743.0, "evalue": 4.31e-265, "gene_start": 1, "gene_end": 414, "reference_start": 302, "reference_end": 713, "reference_sequence": "NNKTNWFDNNNVDWGENKKKQCDENQLGYKETTSTNSHNFHSKSFTQPAYFISGIDSVNDQLIFSGFKAGSVGYDSSSSSSSTKDQALAWSTTTSLDSKTGYRDLVTNDTGLNGPINGSFSIQDTFSFVVPYSMNHTNTGTSGPIKTAYPVKKSEASTVMINSLINATPLNSYGDEGVGVFDALGLNYNFKSNQERLPSRTDQIFVYGIVSPNELRSAKSSADSTGSDTKVNWSNTQSRYLPVPYNYSEGIIDADGFKRPENRGASVTTFSGLKSIAPDGFANSIANFSVGLKAGIDPNPVMSGKKANYGAVVLTRGGVVRLNFNPGNDSLLSTTDNDVAPISFSFTPFTAAESAVDLTTFKEVTYNQESGLWSYVFDNSLKPTHDGKQIAVTDNKGFSVITVSRTGIELNQDQATTTLDVAPSALAVQSGIQSTTQTLTGVLPLSEEFSAVIAKDSGQNKIDIYKNNNGLFEIDTQLSNSVATNNGGLAPSYTENTVDAWGKVEFADNSVLQARNLVDKTVDEIINTPEILNSFFRFTPAFEDQKATLVATKQSDTSLSVSPRIQFLDGNFYDLNSTIAGVPLNIGFPSRVFAGFAALPAWVIPVSVGSSVGILFILLVLGLGIGIPMYRVRKLQDASFVNVFKKVDTLTTAVGSVYKKIITQTGVVKKAPSALKAANPSVKKPAAKPPVQPPSKPEGEQKAVEVKSEETKS" }, "paralog": false, "identity": 0.9708171206225681, "score": 962.0, "evalue": 0.0, "description": "Internal stop codon at 227274. Nonsense mutation around 227274.", "psc": { "uniref90_id": "UniRef90_UPI002FCE5F12", "product": "adhesin P110", "uniref50_id": "UniRef50_UPI0015882A22", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FCE5F12", "UniRef:UniRef50_UPI0015882A22" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0015882A22", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0015882A22" ], "product": "Mgp-operon protein 3" } }, "id": "KMADJIBOEG_456", "locus": "GCA000292445_00420" }, { "type": "cds", "sequence": "contig_1", "start": 229119, "stop": 229385, "strand": "+", "frame": 3, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49237", "UniRef:UniRef90_UPI0002D2A9DB" ], "nt": "GTGCCTAAGGTGGTATTAGAGTACCACAAGCTCAGTAAGGATGTAGTCAAAGAGAGTTTGGAAGTGGAATCTTCTTCCTCCTTCAACCCCACCCAAAGGTTGAAAAAAGATAGTCCAATGAAGGATTCAAGTAAACAAGGGGAGAAACTCAGTGAAGCAACTTCATCGATGAGTAGTAGGGCTACCTCTCCTCGCAAGGCCCTCAAGGTTGAGGTGGAGAAAGGGAAGTTGGATGCGAGTGGGGAGTTTGCCAATGATAAAGCCTGA", "aa": "MPKVVLEYHKLSKDVVKESLEVESSSSFNPTQRLKKDSPMKDSSKQGEKLSEATSSMSSRATSPRKALKVEVEKGKLDASGEFANDKA", "aa_hexdigest": "a87f3bee121a742eaa618943b9bdc30f", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0002D2A9DB", "query_cov": 0.9659090909090909, "subject_cov": 0.8173076923076923, "identity": 0.988, "score": 149.0, "evalue": 5.23e-45, "valid": true, "product": "adhesin", "uniref50_id": "UniRef50_Q49237" }, "pscc": { "uniref50_id": "UniRef50_Q49237", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49237" ], "product": "MgPa" }, "genes": [], "id": "KMADJIBOEG_457", "locus": "GCA000292445_00421" }, { "type": "cds", "sequence": "contig_1", "start": 229399, "stop": 229542, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGAAAAAGTGGGGAGGGGGGTATTTGAACTAACAGAAAGGGTAGCAGTGTGGGAGTTGGAGAAGGGTTTACCATTGCCGATTCAGGGGAGAAAGTCTAAGTCGCCCCCATCCCCCTATTATCTTTTATTTGTTCGGTAG", "aa": "MLEKVGRGVFELTERVAVWELEKGLPLPIQGRKSKSPPSPYYLLFVR", "aa_hexdigest": "daf6ff2bfef8a014def94028c57ad347", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5415.357100000001, "isoelectric_point": 9.693224525451662 }, "id": "KMADJIBOEG_458", "locus": "GCA000292445_00422" }, { "type": "cds", "sequence": "contig_1", "start": 229571, "stop": 229837, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAGTCAATGCATTGCTCCAGTCACTGGTGGGACTGATACTGTTGGGTAAAAAATCATATGTAGTTTTTTTGGGGGAGCTACCTGATGTGACTTGGTTTTCAGAAGAATTGGGATTAAACGTGTTGCTACCATTACTAGGTTTCACCTGTTCAAAAGGAATGCCGTTATTGTTACCTTGTTTGCGATACCCTGCTCCCGTTCAAATGTCTGGTGAAGAGTTAGGGGTAAACACCCACCGGGTGTTCTCTTTATTTAAATGTTGA", "aa": "MKVNALLQSLVGLILLGKKSYVVFLGELPDVTWFSEELGLNVLLPLLGFTCSKGMPLLLPCLRYPAPVQMSGEELGVNTHRVFSLFKC", "aa_hexdigest": "cfbda4d8d7952b0545635a38cf0c4541", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9694.574599999993, "isoelectric_point": 7.713017082214355 }, "id": "KMADJIBOEG_459", "locus": "GCA000292445_00423" }, { "type": "cds", "sequence": "contig_1", "start": 230344, "stop": 230520, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13", "UniRef:UniRef90_UPI0030D43D23" ], "nt": "GTGGTGAAAAAGTTTAAGTTAGATGTGCCAAGTGTTTCAACAACCATGAACGAAGTGTTGCAAAACCTCATCCTTGAACAACCTTTGACTAAGTATACGCTTAATAGTAGTTTGGCTAAACAAAAGGGCAAAAGCCAGATAGAGGTGCATCTGGGTAGTGGGCAAGCAAATAATTGA", "aa": "MVKKFKLDVPSVSTTMNEVLQNLILEQPLTKYTLNSSLAKQKGKSQIEVHLGSGQANN", "aa_hexdigest": "df96736ade49c3b023490562a405e82d", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 230227, "stop": 230585, "observations": { "deletion": [ 230536 ], "mutation": [ 230338, 230518 ], "stop": [ 230338, 230518 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0030D43D23", "query_cov": 0.9655172413793104, "subject_cov": 0.4628099173553719, "identity": 0.821, "score": 93.6, "evalue": 2.1e-23, "gene_start": 1, "gene_end": 56, "reference_start": 42, "reference_end": 97, "reference_sequence": "MKLTYSSSGSNTNTISFENNANKPSYVVEFTNSTNVGIKWRVVKKYQLDVPNVSSDMNQVLQELILEQPLTKYTLNSSLAKQKGKSKIEVHLGSGRATQWTSRTQLHWPKQQSQPKCINRI" }, "paralog": false, "identity": 0.7107438016528925, "score": 158.0, "evalue": 1.65e-51, "description": "Internal stop codon at 230338, 230518. Frameshift due to deletion around 230536. Nonsense mutation around 230338, 230518.", "psc": { "uniref90_id": "UniRef90_UPI0030D43D23", "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0030D43D23", "UniRef:UniRef50_UPI00027B3D13" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6400.338600000001, "isoelectric_point": 9.523801612854005 }, "id": "KMADJIBOEG_460", "locus": "GCA000292445_00424" }, { "type": "cds", "sequence": "contig_1", "start": 231852, "stop": 232412, "strand": "+", "frame": 3, "gene": "pheS", "product": "phenylalanine--tRNA ligase subunit alpha", "db_xrefs": [ "COG:COG0016", "COG:J", "EC:6.1.1.20", "GO:0000049", "GO:0000166", "GO:0000287", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432", "KEGG:K01889", "SO:0001217", "UniRef:UniRef50_P75564", "UniRef:UniRef90_P47436" ], "nt": "ATGCTACCAGCAACAGAGTTTACCAATGGTTCTAATAACGCACTATATCAGGTCATTGATAATTTAGTTGAATACTTTAAAAGCTTTTTATTCACAATTAATTTTGATAGTGAACTGACCAGTATTAGTGACTGTTTTGATCTTTTAAATATCCCTAAAGATCATTCCAGTAGGAATGAATCTGATTCTTTTTATATCGATAAAACCAGTTTATTGAGAACCCATTGTACTGCTACCACGCTAAAAGCAGTCAGAACTTCTAAAAAAACTAATAATCCTGATATCAGGGTTGTCTCTTTAGGAGCGGTTTTTCGTAATGATAGTGATGATGCCACCCACTCCCATCAGTTTACCCAACTTGATTTTATGTGGATTAAAAAAGGGCTTTCATTAGCTAATTTAAAGTGGTTTATTAACAATATGATCACCCATTTCTTTGGGGAAAATACTTTTACTAGGTTTAGACTATCCCACTTCCCATTCACTGAACCCTCGTTTGAAATTGACATTAGGTGTTGGTTATGTCAAAATGGTTGTTCTATTTGTAAGCAAACCAAGTGA", "aa": "MLPATEFTNGSNNALYQVIDNLVEYFKSFLFTINFDSELTSISDCFDLLNIPKDHSSRNESDSFYIDKTSLLRTHCTATTLKAVRTSKKTNNPDIRVVSLGAVFRNDSDDATHSHQFTQLDFMWIKKGLSLANLKWFINNMITHFFGENTFTRFRLSHFPFTEPSFEIDIRCWLCQNGCSICKQTK", "aa_hexdigest": "bf61341790ccddf0dd3cf8ca71deaf9c", "start_type": "ATG", "rbs_motif": null, "genes": [ "pheS" ], "truncated": "both", "pseudogene": { "start": 231579, "stop": 232604, "observations": { "mutation": [ 231609, 232410 ], "stop": [ 231609, 232410 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47436", "query_cov": 1.0, "subject_cov": 0.5454545454545454, "identity": 1.0, "score": 377.0, "evalue": 2.77e-130, "gene_start": 1, "gene_end": 186, "reference_start": 92, "reference_end": 277, "reference_sequence": "MIDQNKLITKWKKAFAKAKNLTTLVNLKNTLHNSDLKPLLQKIKTATKLSEKSSLGKLYQSLDIQLTDLLTSYKKTFEINNQVSQKPSLDVMLPATEFTNGSNNALYQVIDNLVEYFKSFLFTINFDSELTSISDCFDLLNIPKDHSSRNESDSFYIDKTSLLRTHCTATTLKAVRTSKKTNNPDIRVVSLGAVFRNDSDDATHSHQFTQLDFMWIKKGLSLANLKWFINNMITHFFGENTFTRFRLSHFPFTEPSFEIDIRCWLCQNGCSICKQTKWIEILGAGIIHPQVMNNMGIGDTENITGIAAGIGIERLAMLKYGIDDIRDFYDNNFKFLTQFTD" }, "paralog": false, "identity": 0.9941348973607038, "score": 681.0, "evalue": 2.12e-252, "description": "Internal stop codon at 231609, 232410. Nonsense mutation around 231609, 232410.", "psc": { "uniref90_id": "UniRef90_P47436", "gene": "pheS", "product": "phenylalanine--tRNA ligase subunit alpha", "ec_ids": [ "6.1.1.20" ], "uniref50_id": "UniRef50_P75564", "cog_id": "COG0016", "cog_category": "J", "kegg_orthology_id": "K01889", "go_ids": [ "GO:0000049", "GO:0000166", "GO:0000287", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47436", "EC:6.1.1.20", "UniRef:UniRef50_P75564", "COG:COG0016", "COG:J", "KEGG:K01889", "GO:0000049", "GO:0000166", "GO:0000287", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432" ] }, "pscc": { "uniref50_id": "UniRef50_P75564", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75564" ], "product": "Phenylalanine--tRNA ligase alpha subunit" } }, "id": "KMADJIBOEG_461", "locus": "GCA000292445_00425" }, { "type": "cds", "sequence": "contig_1", "start": 232452, "stop": 232604, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01409.25" ], "nt": "ATGAATAACATGGGAATTGGGGATACTGAAAATATTACTGGGATAGCAGCAGGAATTGGGATTGAACGCTTAGCAATGTTAAAGTATGGGATTGATGATATCCGTGATTTTTATGATAACAACTTTAAGTTTTTAACCCAGTTTACTGACTAA", "aa": "MNNMGIGDTENITGIAAGIGIERLAMLKYGIDDIRDFYDNNFKFLTQFTD", "aa_hexdigest": "c10ad6a6cdb630eab0435e14e5630067", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01409.25", "name": "tRNA-synt_2d", "length": 47, "aa_cov": 0.94, "hmm_cov": 0.9574468085106383, "evalue": 7.087926384644626e-16, "score": 53.52961349487305, "start": 2, "stop": 48 } ], "seq_stats": { "molecular_weight": 5634.288600000001, "isoelectric_point": 4.293526649475097 }, "id": "KMADJIBOEG_462", "locus": "GCA000292445_00426" }, { "type": "cds", "sequence": "contig_1", "start": 232608, "stop": 233081, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01588.26" ], "nt": "ATGTTGATATCAAAAAAAACACTTGGCGTTTTAATCCCTGATATCTTTAGTTTTTCTAATGATCAAATTGCCCAAAAGTTAGAACAAATGGGGATTGAAGTGGAATCAATTAAGCAGTTTAACAGCCCTGATTACCTCCAACTTGCAAAGGTTGTATCAATCCAACCCCATCCCCATGACAACAAGCTTTTTATCTGTGAATTACAAATTGATAAAAACAAGTTTATTAATGTTGTTTCCAATGCTAATAACATTAACAATCCTGATAATATCAACAAGTTTGTCATTGTTGCAAAAAAAGGAACTGAGTTACTCAACGGGTTAATTGTTAAAACCCAAAATATTAAAGGGATCATTTCAGAAGGGATTTTATGTAGCTATATTGACATTAACCCCTTCAGTAGACAGATCATTGAAAAAACAGAAGTTGCTGATGCGATTATCATTGATCATGTTAGCAATGATCATGACTGA", "aa": "MLISKKTLGVLIPDIFSFSNDQIAQKLEQMGIEVESIKQFNSPDYLQLAKVVSIQPHPHDNKLFICELQIDKNKFINVVSNANNINNPDNINKFVIVAKKGTELLNGLIVKTQNIKGIISEGILCSYIDINPFSRQIIEKTEVADAIIIDHVSNDHD", "aa_hexdigest": "0376e536e714d56cc3e409b342b40556", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01588.26", "name": "tRNA_bind", "length": 94, "aa_cov": 0.5987261146496815, "hmm_cov": 0.9148936170212766, "evalue": 8.782840120297826e-14, "score": 46.59867858886719, "start": 47, "stop": 140 } ], "seq_stats": { "molecular_weight": 17611.09349999999, "isoelectric_point": 5.525227546691894 }, "id": "KMADJIBOEG_463", "locus": "GCA000292445_00427" }, { "type": "cds", "sequence": "contig_1", "start": 233115, "stop": 233342, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCTTTGATGTTAAAACCCCAACTAACAGAGCAGATCTTCATAGCTTAATCTTTTTAGCAAAAGAACTTGGGGTACTTTTGAAAACCAAAACCTTTTTAAAACAAAAAAGTAGTGTTGTTAACCATGACTTTTTTAAGTTTCCCCTAAATTTAAAAAACAAGTTAAAAGCGAATTATTTTGGCGGTTTGTTCTTAAGACAAATTAACCAACATAGTTCACCTTGA", "aa": "MIFDVKTPTNRADLHSLIFLAKELGVLLKTKTFLKQKSSVVNHDFFKFPLNLKNKLKANYFGGLFLRQINQHSSP", "aa_hexdigest": "c256bba9dff5f863cb54becac8b7c18a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8648.089899999997, "isoelectric_point": 10.237402153015136 }, "id": "KMADJIBOEG_464", "locus": "GCA000292445_00428" }, { "type": "cds", "sequence": "contig_1", "start": 233370, "stop": 234014, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03483.22" ], "nt": "ATGATCAAACCAGTTAACTATTATGTTGATAAAGCTAACTTAGTAACAGTGTTCACCGCTCAGCCAATCCATTGTCATGATGCAGATAGAATTGTTGGTAACATTGAACTTAAACAAGCAACCCATAATGAAACTTTTGTTGGACTTGATGACAAGCAATATGAGATTGAACCAGGGGATATTGTTGTTTGTGATGAGAAGGGCATTATTGCACTGGTAGGGATCATTGGTTCAAAGCGCACAATGGTCCAACCTACAACAACTAACATCTTTTTTGAAGTTGTTAACTGTAACAGTGAAACCATTAAACAAACTGCCAAGCGCTTTTTGATCAATAACTTTGCCAGTAAGTTTATGGTTAAACCGATTAGCTTATTAGCTACTGATAACTGTTTAAACTACTTACAAAACAGTTTACTAACCACTGATAACATTGGCAAAATTAGCCACTTTTCAAGTTCGCTTAAAGTTGAACCATTTAGTAAAAAGCTCACAGTGAATTTCCATAAGATACGCCAACTAATTGGCATTGAAAAAAAGGAACTAACTGATCAAACCATTAAAAAAAGCCTCAGTCAACTAGGGTTTAAAGTTGACAACCAACTTCTCAAAATCCCCAGTTACAGACAAGACATTAATACTTGA", "aa": "MIKPVNYYVDKANLVTVFTAQPIHCHDADRIVGNIELKQATHNETFVGLDDKQYEIEPGDIVVCDEKGIIALVGIIGSKRTMVQPTTTNIFFEVVNCNSETIKQTAKRFLINNFASKFMVKPISLLATDNCLNYLQNSLLTTDNIGKISHFSSSLKVEPFSKKLTVNFHKIRQLIGIEKKELTDQTIKKSLSQLGFKVDNQLLKIPSYRQDINT", "aa_hexdigest": "2e3fc53a1a13632e33e2a11b1dc81f15", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03483.22", "name": "B3_4", "length": 140, "aa_cov": 0.6542056074766355, "hmm_cov": 1.0, "evalue": 3.1658074283514254e-41, "score": 135.9696044921875, "start": 2, "stop": 141 } ], "seq_stats": { "molecular_weight": 24162.667700000024, "isoelectric_point": 8.787379264831543 }, "id": "KMADJIBOEG_465", "locus": "GCA000292445_00429" }, { "type": "cds", "sequence": "contig_1", "start": 234036, "stop": 234419, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17759.6" ], "nt": "GTGAAGTTAATTGATATCAATAAGTTAAAACCAATTGGGATCACTAGTAGTTTTAACTTTGAAAAGTCTAGTTACTTTAACACTTTTAATGCTTTAACAAAACTAAGAAAAAAACTGCAAACACTTGGTTTTCACAACGTTATTACCTACCAGTTAACTGATCAAAAAAGTGCAAAAACTTTTAATTTGTTTAACTTAGAAAATTTCATCACCATTAAAAACCCAGTGTCCCAAAACCATTCTGTAATGCGTGTTAGCTTAATTGATTCACTGTTAAAAGTGCTACAAACCAATAACAACTATAAGAATGAACTGGTGAACATCTTTGAGTTTTCCTTTATTAAAACCCAAAACAATAGTGAACTGCACCTGGCAGTATTATGA", "aa": "MKLIDINKLKPIGITSSFNFEKSSYFNTFNALTKLRKKLQTLGFHNVITYQLTDQKSAKTFNLFNLENFITIKNPVSQNHSVMRVSLIDSLLKVLQTNNNYKNELVNIFEFSFIKTQNNSELHLAVL", "aa_hexdigest": "95ba04f1fd78526260de85933ef6a149", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17759.6", "name": "tRNA_synthFbeta", "length": 90, "aa_cov": 0.7007874015748031, "hmm_cov": 1.0, "evalue": 1.9745601748376104e-21, "score": 71.59088134765625, "start": 30, "stop": 118 } ], "seq_stats": { "molecular_weight": 14717.84769999999, "isoelectric_point": 9.77703342437744 }, "id": "KMADJIBOEG_466", "locus": "GCA000292445_00430" }, { "type": "cds", "sequence": "contig_1", "start": 234453, "stop": 234854, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17759.6" ], "nt": "ATGCAAGGGATAAGCAATGATGTTTTTACTATGAAGGGATTAGCAAAACTCATTGTTGCTAACTTAGGGTTTAGTTGTGACTTTGAACCACTTGATGATAGTGACTATTTTGTTAATAATCAAAGTTTAAAAATAGTAGTTTTTAACGAACAGATCGGTTTTATTGGGCTAATTAAAGAATCATTGTTAAATAACTATGATCTGAACAATAAACCCATTTATTGTCTTGAAATCAACTTAGATAGGATGCTCTTTTCTCTAAACAGGATTGAAAAAAACTACCTTGGTTACAGTAAACTACAACCTGTTTGCAAGGATCTTACCTTTAGTTTTACCAACCCTGCTAGTCACTTTGATCAGTTTGCTAACATGATCAAAAGGATAACTGGCATTGAAAGTTGA", "aa": "MQGISNDVFTMKGLAKLIVANLGFSCDFEPLDDSDYFVNNQSLKIVVFNEQIGFIGLIKESLLNNYDLNNKPIYCLEINLDRMLFSLNRIEKNYLGYSKLQPVCKDLTFSFTNPASHFDQFANMIKRITGIES", "aa_hexdigest": "549134cf8478a701f1359f781bdbe8cd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17759.6", "name": "tRNA_synthFbeta", "length": 76, "aa_cov": 0.5639097744360902, "hmm_cov": 0.9736842105263158, "evalue": 1.1058218659425805e-13, "score": 46.2684440612793, "start": 6, "stop": 80 } ], "seq_stats": { "molecular_weight": 15205.324799999984, "isoelectric_point": 5.035218620300292 }, "id": "KMADJIBOEG_467", "locus": "GCA000292445_00431" }, { "type": "cds", "sequence": "contig_1", "start": 234879, "stop": 235028, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAACAACCAACTGATCACTAAGTACACCGTTCGTTATTTTCTGAAAAATGATGCTAACAAACCACTAACTAACCAAACAATTGAACTTATCACTAATAACTTAAAACTCCAGTGTGAAAAACTAAAAATTAAATTAGATATTTAG", "aa": "MQNNQLITKYTVRYFLKNDANKPLTNQTIELITNNLKLQCEKLKIKLDI", "aa_hexdigest": "758432b39fa1512bcb857ab864ede4d7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5822.796399999998, "isoelectric_point": 9.51832180023193 }, "id": "KMADJIBOEG_468", "locus": "GCA000292445_00432" }, { "type": "cds", "sequence": "contig_1", "start": 235087, "stop": 235632, "strand": "+", "frame": 1, "gene": "infC", "product": "translation initiation factor IF-3", "db_xrefs": [ "COG:COG0290", "COG:J", "GO:0003743", "GO:0005829", "GO:0006413", "GO:0032790", "GO:0043022", "RefSeq:WP_009885737.1", "SO:0001217", "UniParc:UPI0000165F7E", "UniRef:UniRef100_UPI0000165F7E", "UniRef:UniRef50_P47438", "UniRef:UniRef90_P47438" ], "nt": "TTGGCACAAAACGCAAAACACCCTTCTAAAAAAGAACAAAAACCACTCGTTAATGAACAGATTGCTTTCAATCAGTTCACTTTAATTGATGAAAACAGTACTAATTTAGGGATAGTTAAGATGGAAAACGCTTTAAAGTTAGCACAAGAAAAACAGTTAGATCTAGTTCTAATTGCTCCAAACCCAACCAAACCGATCGTTAAGTTGTTGGACTTTGGCAGATATACCTATGATTTAAAGCGTAAGAAAAGACAAGCCAAGAAAAACCAAACAATCATCCAAACCAAAGAAGTTGTTGTCAAACCAACGATTGCTAAACATGATTTAGAATTTAGAGCAAAACAGAGTAAGAATTGGATAGAAAAAGGTCATCATGTCAAGTTTATAGTCCGTGCCTTTGGCAGGGTTAGCACCAGGATAGAGTTAATTGAAAAGGTGTTTGATGACTTTTACCAGTTAGTTAAAGATGTAGTTGAGATCCAAAAACCTTTAACCGCTTCTTCCAAAACGATGTACGCTGCTCTATTAGTACCTTTAAAAAGATAG", "aa": "MAQNAKHPSKKEQKPLVNEQIAFNQFTLIDENSTNLGIVKMENALKLAQEKQLDLVLIAPNPTKPIVKLLDFGRYTYDLKRKKRQAKKNQTIIQTKEVVVKPTIAKHDLEFRAKQSKNWIEKGHHVKFIVRAFGRVSTRIELIEKVFDDFYQLVKDVVEIQKPLTASSKTMYAALLVPLKR", "aa_hexdigest": "10d5dec888ea3d6eadcff8182f502af4", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000165F7E", "ncbi_nrp_id": "WP_009885737.1", "uniref100_id": "UniRef100_UPI0000165F7E", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000165F7E", "RefSeq:WP_009885737.1", "UniRef:UniRef100_UPI0000165F7E" ] }, "ips": { "uniref100_id": "UniRef100_UPI0000165F7E", "uniref90_id": "UniRef90_P47438" }, "psc": { "uniref90_id": "UniRef90_P47438", "gene": "infC", "product": "translation initiation factor IF-3", "uniref50_id": "UniRef50_P47438", "cog_id": "COG0290", "cog_category": "J", "go_ids": [ "GO:0003743", "GO:0005829", "GO:0006413", "GO:0032790", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_P47438", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47438" ], "product": "Translation initiation factor IF-3" }, "genes": [ "infC" ], "id": "KMADJIBOEG_469", "locus": "GCA000292445_00433" }, { "type": "cds", "sequence": "contig_1", "start": 235635, "stop": 235814, "strand": "+", "frame": 3, "gene": "rpmI", "product": "50S ribosomal protein L35", "db_xrefs": [ "COG:COG0291", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904", "KEGG:K02916", "RefSeq:WP_009885738.1", "SO:0001217", "UniParc:UPI0000133FF7", "UniRef:UniRef100_P47439", "UniRef:UniRef50_P47439", "UniRef:UniRef90_P47439" ], "nt": "ATGAAAACCAAAAGTGCTGCAGTAAAACGCTTTAAACTCACCAAATCAGGACAAATTAAGCGCAAACACGCTTATACTTCCCACCTCGCGCCCCACAAATCAACCAAACAAAAGCGCCATTTGCGCAAGCAAGCTACTGTGAGCAACAGTGAATTGAAAAGAATTGGTATTTTAATTTAG", "aa": "MKTKSAAVKRFKLTKSGQIKRKHAYTSHLAPHKSTKQKRHLRKQATVSNSELKRIGILI", "aa_hexdigest": "a92c22b563615ebee0f1d0ca7619ae8f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133FF7", "ncbi_nrp_id": "WP_009885738.1", "uniref100_id": "UniRef100_P47439", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133FF7", "RefSeq:WP_009885738.1", "UniRef:UniRef100_P47439" ] }, "ips": { "uniref100_id": "UniRef100_P47439", "uniref90_id": "UniRef90_P47439" }, "psc": { "uniref90_id": "UniRef90_P47439", "gene": "rpmI", "product": "50S ribosomal protein L35", "uniref50_id": "UniRef50_P47439", "cog_id": "COG0291", "cog_category": "J", "kegg_orthology_id": "K02916", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P47439", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47439" ], "product": "Large ribosomal subunit protein bL35" }, "genes": [ "rpmI" ], "id": "KMADJIBOEG_470", "locus": "GCA000292445_00434" }, { "type": "cds", "sequence": "contig_1", "start": 235817, "stop": 236191, "strand": "+", "frame": 2, "gene": "rplT", "product": "50S ribosomal protein L20", "db_xrefs": [ "COG:COG0292", "COG:J", "GO:0000027", "GO:0000315", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022625", "RefSeq:WP_014894211.1", "SO:0001217", "UniParc:UPI00027B3ABB", "UniRef:UniRef100_UPI00027B3ABB", "UniRef:UniRef50_Q7NBC0", "UniRef:UniRef90_P47440" ], "nt": "ATGCGTGTTAAGGGAACAAATACAACCAGGATTAGAAGAAAAAAATGGTTAAAACAAGCTAGTGGTAGCTTTGGGACAAGAAAAGCTTCTTTTAAAGCAGCTAAACAAACTGTTATCCAAGCAAGCAAGTATGCTTACCGTGATAGGAGACAGAAAAAACGTGAGTTTCGTTCGTTGTGGATCTTAAGGTTAAATGCTGCACTGCGTGCACAAGGGATGACTTATTCAGTGTTTATCAATGAATTGAAAAAAGCCAAGATAGCCATTAACAGAAAGGTACTTTCTGAACTAGCAATTAAAGAACCTAATAAGTTAAATCTGATTATCAATACCATCAAAAAACCAACTAATAAACCAACTGTTGCAAAAACTTAG", "aa": "MRVKGTNTTRIRRKKWLKQASGSFGTRKASFKAAKQTVIQASKYAYRDRRQKKREFRSLWILRLNAALRAQGMTYSVFINELKKAKIAINRKVLSELAIKEPNKLNLIINTIKKPTNKPTVAKT", "aa_hexdigest": "7c9d6a4baf9d022ad62dd699c2c619ec", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3ABB", "ncbi_nrp_id": "WP_014894211.1", "uniref100_id": "UniRef100_UPI00027B3ABB", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3ABB", "RefSeq:WP_014894211.1", "UniRef:UniRef100_UPI00027B3ABB" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3ABB", "uniref90_id": "UniRef90_P47440" }, "psc": { "uniref90_id": "UniRef90_P47440", "gene": "rplT", "product": "50S ribosomal protein L20", "uniref50_id": "UniRef50_Q7NBC0", "cog_id": "COG0292", "cog_category": "J", "go_ids": [ "GO:0000027", "GO:0000315", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q7NBC0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q7NBC0" ], "product": "Large ribosomal subunit protein bL20" }, "genes": [ "rplT" ], "id": "KMADJIBOEG_471", "locus": "GCA000292445_00435" }, { "type": "cds", "sequence": "contig_1", "start": 236163, "stop": 236666, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCACCATACAATTACATTAGATCCCAAGCAGTATAATGATTTGACAAAATCACTGAAAAATACCAATTTACTTTTGACCAATTTACACTGTCAGCTTTACCAAAAGTTACTGGAAAAAAATCAGTTATTAAGACAAACAGTAACAATTAGCATTGACCAGTTTGCCAATCAAGAGTTATTTGTTAACTATCTAAATAAGCTAACTAATTTCACTGATAAAACAGTGTTATTACCTGATCAGTTTCTAATTAATGGTGAAACTAAGTCACTGGTGATTGCAGCTGCATCTATCTTAGCAAGAGATAGCTTTATTAATCAGCTGCAATTACTCAACCAAAAGGTAAACTATGATTTACCCAAAGGCAGTAGTCATGGCATTGAACAAGCGTTGTTATTTCTTAACCAACAACGCGGTTTTAGTCAAATTGAACAGTTTAAACAGGTGGCAAAACTTAACTTTAAAAACGTTACTAAGTTTTTGCAACAGTTGGTTTATTAG", "aa": "MDHHTITLDPKQYNDLTKSLKNTNLLLTNLHCQLYQKLLEKNQLLRQTVTISIDQFANQELFVNYLNKLTNFTDKTVLLPDQFLINGETKSLVIAAASILARDSFINQLQLLNQKVNYDLPKGSSHGIEQALLFLNQQRGFSQIEQFKQVAKLNFKNVTKFLQQLVY", "aa_hexdigest": "b11d745e642ca5c2568737c9a3077ee3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19311.005999999987, "isoelectric_point": 9.162714195251464 }, "id": "KMADJIBOEG_472", "locus": "GCA000292445_00436" }, { "type": "cds", "sequence": "contig_1", "start": 236732, "stop": 236869, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAACATTACAAACCAGCTGATTTTTATTTAATTGGCAGTGATGAATCTGGCAAGGGTGATAGTTTTGGTGGGATTTGTGTTAGTGCTGTTTTAATTGAAAAAAACAGCTTATTAATCTTGAAAATTTACAAGTAA", "aa": "MQHYKPADFYLIGSDESGKGDSFGGICVSAVLIEKNSLLILKIYK", "aa_hexdigest": "903d035f25f3f54967ef8fecfed6a6fc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4949.6961999999985, "isoelectric_point": 6.516954231262207 }, "id": "KMADJIBOEG_473", "locus": "GCA000292445_00437" }, { "type": "cds", "sequence": "contig_1", "start": 236916, "stop": 237311, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00226.36" ], "nt": "ATGGCTGAACAGAAACGTGATTATTATGAAGTGTTAGGAATAACCCCTGATGCTGATCAATCAGAGATTAAAAAAGCCTTTCGTAAGCTGGCTAAAAAGTACCATCCTGATCGTAACAACGCGCCTGATGCTGCTAAGATTTTTGCTGAAATTAATGAAGCAAATGATGTTTTATCAAACCCCAAAAAAAGAGCTAACTATGATAAGTATGGTTTTGATGGGGTTGATGGCGAACCTGCTTTTAACTTCCAAGCAGATGTTTTTCAATCCTTTTTTGAAGAGATAGCAAAATCAGGGGTTTTTAACAACCAAACCAATCCTGAACAAAAAGAAAAAAGAAACGTTACCACTGGTTCTCCAAAAAACCTAAGCAAGAACAACCTGAAATTAACTTAG", "aa": "MAEQKRDYYEVLGITPDADQSEIKKAFRKLAKKYHPDRNNAPDAAKIFAEINEANDVLSNPKKRANYDKYGFDGVDGEPAFNFQADVFQSFFEEIAKSGVFNNQTNPEQKEKRNVTTGSPKNLSKNNLKLT", "aa_hexdigest": "76ffdd33f98465c4b38c01e63809c587", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00226.36", "name": "DnaJ", "length": 63, "aa_cov": 0.4732824427480916, "hmm_cov": 1.0, "evalue": 9.259895560789945e-29, "score": 94.5582504272461, "start": 7, "stop": 68 } ], "seq_stats": { "molecular_weight": 14880.321499999993, "isoelectric_point": 7.841628074645997 }, "id": "KMADJIBOEG_474", "locus": "GCA000292445_00438" }, { "type": "cds", "sequence": "contig_1", "start": 237341, "stop": 237481, "strand": "+", "frame": 2, "gene": null, "product": "DnaJ-like protein MG200", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002AA2D45", "UniRef:UniRef90_UPI0002AA2D45" ], "nt": "GTGCAACAAAACCAAAACCAAAACAAAGACCCAGATGAATTACGTTCTAAGGTCCCTGGAGAGGTTACTGCTAGTGATTGGGAAGCATTGGTTGGTGATACTAGGTATGGGTATTTTGATGAAACAGGGGATTGGAGTTGA", "aa": "MQQNQNQNKDPDELRSKVPGEVTASDWEALVGDTRYGYFDETGDWS", "aa_hexdigest": "daaead17ad282b9b33914626d5e66ce9", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 237284, "stop": 237538, "observations": { "mutation": [ 237479, 237515 ], "stop": [ 237479, 237515 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0002AA2D45", "query_cov": 1.0, "subject_cov": 0.5, "identity": 0.978, "score": 95.9, "evalue": 6.84e-25, "gene_start": 1, "gene_end": 46, "reference_start": 20, "reference_end": 65, "reference_sequence": "KQEQPEINLDHVVEQTIKKVQQNQNQNKDPDELRSKVPGEVTASDWEALVGDTRYGYFDETGDWSWKGYFDEQGKWVWNEPVDSLEHHHHHH" }, "paralog": false, "identity": 0.9761904761904762, "score": 146.0, "evalue": 1.56e-47, "description": "Internal stop codon at 237479, 237515. Nonsense mutation around 237479, 237515.", "psc": { "uniref90_id": "UniRef90_UPI0002AA2D45", "product": "DnaJ-like protein MG200", "uniref50_id": "UniRef50_UPI0002AA2D45", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0002AA2D45", "UniRef:UniRef50_UPI0002AA2D45" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0002AA2D45", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002AA2D45" ], "product": "DnaJ-like protein MG200" } }, "id": "KMADJIBOEG_475", "locus": "GCA000292445_00439" }, { "type": "cds", "sequence": "contig_1", "start": 237447, "stop": 238673, "strand": "+", "frame": 3, "gene": "dnaJ", "product": "DnaJ C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002E59EF3", "UniRef:UniRef90_UPI0002E59EF3" ], "nt": "ATGGGTATTTTGATGAAACAGGGGATTGGAGTTGAAAGGGTTACTTTGATGAACAGGGCAAATGGGTTTGAAACGAACCAGTTGATTCTGAAACCAGTGAGGTATCAGTTGAACCTGAACCAACCCCAGTTGCCCCTGAAGCTAGTTTTGAAGAAGCTCAACCTGAAACCAGTTGCCCCTGAAGCTAGTTTTGAAGAAGCTCAACCTGAACCAACTCCAATTCCTGAACCAATCCCAACCCCAGTTCAAGTTCAACCCCTGTTGTTAGATCTCAACCTCTTTACTATCCCAACTAAAGCTACTAAGGATGATCTTTTGTTTGACAACATTAACCTCACTACCTATGAACAAGTTGTTGATTATCTCAACAGTCAAGCAACCCCTAATTTAGCTAAAACCGATGGTGAATTGCAAACGATTGATGGTACCAACCCATTGTTATTAGAACAGTGCAAAAAGATCAAAAAACAAGCAGAACAACTCTTTAAAAAACTCTTTTTAAAAAAACAACTCCCCTTCATCACCCAACCTGAAGTTGTTGAGGAAAGTAAAACCAGTTTTGATGAGAACAACGTTAACCTTGTTTACTTTGAAAAGGTCCCTGAAATCCTTTTCATTAACCAACAACCTAAGGAGGTAAAATACACCCGTCAAGTCTTTGATGGGTTGACAAACAAAACAACTAGTGAAACGATTACACTAGAGATCCAACTACTCCAAACCCCAAAAGAGACTGTTAGTGCCATTTTTAAAGGCTTTGGTAATGACCATGGCAAGGGCTGTGGGGATTTAAAGATTGTTTTTGAAAAGATTAAAAGCCCCTTTTTTCAAGTCAATGAGGATGGCTTGCACTCTGCTTGCATCATTGACCCTTTAGTTGCTTACAACGGCGGGATTATCGATGTGTTTGGGCCCTACACTAACTTCCAAGTTAAGGTAGATGGGGAGATAGATATCAATGCCATTATGAAGTTTGAAAAACTAGGCATTGCTAAAACCAAACGCAAGGGCGATCTTTTTGTCCATCTCTATTACAGTAGTGTCCCTAAAAAGAAACTCACCACTAACCCCCAAGTTCAACAGTTCTTAGAACTTTTACAAGCTGAATATGAACTGTTGCAAGACAACATCAAGAGCTTAAAGTACTTTAAAAATAACCTAGTTATCCCCAAAAAGCCACTTGATCAACAAAGCTATCAATACCTCTCCCAAGAACCCATTAGTTAG", "aa": "MGILMKQGIGVERVTLMNRANGFETNQLILKPVRYQLNLNQPQLPLKLVLKKLNLKPVAPEASFEEAQPEPTPIPEPIPTPVQVQPLLLDLNLFTIPTKATKDDLLFDNINLTTYEQVVDYLNSQATPNLAKTDGELQTIDGTNPLLLEQCKKIKKQAEQLFKKLFLKKQLPFITQPEVVEESKTSFDENNVNLVYFEKVPEILFINQQPKEVKYTRQVFDGLTNKTTSETITLEIQLLQTPKETVSAIFKGFGNDHGKGCGDLKIVFEKIKSPFFQVNEDGLHSACIIDPLVAYNGGIIDVFGPYTNFQVKVDGEIDINAIMKFEKLGIAKTKRKGDLFVHLYYSSVPKKKLTTNPQVQQFLELLQAEYELLQDNIKSLKYFKNNLVIPKKPLDQQSYQYLSQEPIS", "aa_hexdigest": "3e3fc6c3bed2b6a16dc0908fa265f195", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0002E59EF3", "query_cov": 0.8799019607843137, "subject_cov": 1.0, "identity": 0.997, "score": 670.0, "evalue": 1.05e-241, "valid": true, "product": "DnaJ C-terminal domain-containing protein", "uniref50_id": "UniRef50_UPI0002E59EF3" }, "pscc": { "uniref50_id": "UniRef50_UPI0002E59EF3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002E59EF3" ], "product": "DnaJ C-terminal domain-containing protein" }, "genes": [ "dnaJ" ], "id": "KMADJIBOEG_476", "locus": "GCA000292445_00440" }, { "type": "cds", "sequence": "contig_1", "start": 238685, "stop": 239137, "strand": "+", "frame": 2, "gene": "grpE", "product": "Protein GrpE", "db_xrefs": [ "COG:COG0576", "COG:O", "GO:0000774", "GO:0005737", "GO:0006457", "GO:0042803", "GO:0051082", "GO:0051087", "KEGG:K03687", "SO:0001217", "UniRef:UniRef50_P47443", "UniRef:UniRef90_P47443" ], "nt": "ATGTGTGAAAAATCACAAACAATTAAAGAGCTTTTAAACGCCATTAGAACCTTAGTTGTCAAGAACAATAAAGCTAAGGTTAGTATGATTGAAAAGGAACTGTTAGCTTTTGTTAGTGAACTTGACAAAAAGTTCAAACAACAACTCAACAACTTCAATGAACTACAACAAAAGATCCCACTACTCCAAAAAGCTAACGAAGAGTTTGCTTTAAAGTTTGAAAGGATGCAACGCGAAGCACAAAACCAGATCCAAGCCAAACTAGATGAGTTGAATCTTAAAAATAAAAAGGAGTTAGAACAAGCCAAGAAATATGCGATTGCCAAAACCCTTGACCAACCCTTAAACATCATCGATCAGTTTGAAATCGCGCTTTCATATGCCCAAAAAGACCCTCAAGTAAAAAACTATACCACTGGTTTTACCATGGTACTTGATGCTTTTTCAAGGTGA", "aa": "MCEKSQTIKELLNAIRTLVVKNNKAKVSMIEKELLAFVSELDKKFKQQLNNFNELQQKIPLLQKANEEFALKFERMQREAQNQIQAKLDELNLKNKKELEQAKKYAIAKTLDQPLNIIDQFEIALSYAQKDPQVKNYTTGFTMVLDAFSR", "aa_hexdigest": "dcd1c5028af2b4cc9c5a6cc785425d1b", "start_type": "ATG", "rbs_motif": null, "genes": [ "grpE" ], "truncated": "3-prime", "pseudogene": { "start": 238685, "stop": 239338, "observations": { "mutation": [ 239135 ], "stop": [ 239135 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47443", "query_cov": 1.0, "subject_cov": 0.6912442396313364, "identity": 1.0, "score": 275.0, "evalue": 4.01e-92, "gene_start": 1, "gene_end": 150, "reference_start": 1, "reference_end": 150, "reference_sequence": "MCEKSQTIKELLNAIRTLVVKNNKAKVSMIEKELLAFVSELDKKFKQQLNNFNELQQKIPLLQKANEEFALKFERMQREAQNQIQAKLDELNLKNKKELEQAKKYAIAKTLDQPLNIIDQFEIALSYAQKDPQVKNYTTGFTMVLDAFSRWLEANGVTKIKIEPGMEFDEKIMSALELVDSNLAKNKVVRVSKSGYKLYDKVIRFASVFVSKGNKKS" }, "paralog": false, "identity": 0.9953917050691244, "score": 412.0, "evalue": 7.68e-149, "description": "Internal stop codon at 239135. Nonsense mutation around 239135.", "psc": { "uniref90_id": "UniRef90_P47443", "gene": "grpE", "product": "Protein GrpE", "uniref50_id": "UniRef50_P47443", "cog_id": "COG0576", "cog_category": "O", "kegg_orthology_id": "K03687", "go_ids": [ "GO:0005737", "GO:0000774", "GO:0042803", "GO:0051087", "GO:0051082", "GO:0006457" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47443", "UniRef:UniRef50_P47443", "COG:COG0576", "COG:O", "KEGG:K03687", "GO:0005737", "GO:0000774", "GO:0042803", "GO:0051087", "GO:0051082", "GO:0006457" ] }, "pscc": { "uniref50_id": "UniRef50_P47443", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47443" ], "product": "Protein GrpE" } }, "id": "KMADJIBOEG_477", "locus": "GCA000292445_00441" }, { "type": "cds", "sequence": "contig_1", "start": 239138, "stop": 239338, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAGCAAATGGGGTTACCAAGATTAAGATTGAACCAGGGATGGAATTTGATGAAAAGATTATGTCTGCATTGGAACTAGTTGATTCTAACCTTGCTAAAAACAAGGTAGTAAGAGTCTCAAAATCTGGCTATAAACTCTATGACAAAGTGATCCGCTTTGCATCAGTATTTGTCAGCAAAGGTAATAAAAAATCATAA", "aa": "MEANGVTKIKIEPGMEFDEKIMSALELVDSNLAKNKVVRVSKSGYKLYDKVIRFASVFVSKGNKKS", "aa_hexdigest": "e587f55f73e988ad5231e8e176511e67", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7367.566200000002, "isoelectric_point": 9.646807289123537 }, "id": "KMADJIBOEG_478", "locus": "GCA000292445_00442" }, { "type": "cds", "sequence": "contig_1", "start": 239348, "stop": 239716, "strand": "+", "frame": 2, "gene": null, "product": "MPN121 family protein", "db_xrefs": [ "RefSeq:WP_010869370.1", "SO:0001217", "UniParc:UPI0000139460", "UniRef:UniRef100_P47444", "UniRef:UniRef50_P75353", "UniRef:UniRef90_P47444" ], "nt": "ATGAGTGAACAAAAAAGAAGAACAATCCAAATTGCGATAAGTGAAGACCACTATGAAGAGTTACAAAAGGCATTGGAACTACTTAAAGGGACCCAATTACCCTTTTCAACCACTGTTGAACAGTTTGTGGAGTTAATCTTATCTAACTATGTAGCTACTTCCAATAAGATTAGTAGTTTAGCTAAGAGTGGTTTTGATGTAGCTTCATTGCAGCAAGAACTTGAGAAGATAGGTAACCTTAGTGGGGTTGATGATAACCTCAAGGGTTTTCTCTCAGAACTGTTGAAAACCTCAAGGAATGGGTTTAGTAACCCCAATAAAGATGGCAAAAAAAATGATGACGATAATAACTCGTCATCAAAATCATAG", "aa": "MSEQKRRTIQIAISEDHYEELQKALELLKGTQLPFSTTVEQFVELILSNYVATSNKISSLAKSGFDVASLQQELEKIGNLSGVDDNLKGFLSELLKTSRNGFSNPNKDGKKNDDDNNSSSKS", "aa_hexdigest": "864cae9723138c15b3bad2d2b444adbb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139460", "ncbi_nrp_id": "WP_010869370.1", "uniref100_id": "UniRef100_P47444", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139460", "RefSeq:WP_010869370.1", "UniRef:UniRef100_P47444" ] }, "ips": { "uniref100_id": "UniRef100_P47444", "uniref90_id": "UniRef90_P47444" }, "psc": { "uniref90_id": "UniRef90_P47444", "product": "MPN121 family protein", "uniref50_id": "UniRef50_P75353" }, "pscc": { "uniref50_id": "UniRef50_P75353", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75353" ], "product": "Uncharacterized protein MG202 homolog" }, "genes": [], "id": "KMADJIBOEG_479", "locus": "GCA000292445_00443" }, { "type": "tRNA", "sequence": "contig_1", "start": 239734, "stop": 239807, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(ggt)", "amino_acid": "Thr", "anti_codon": "ggt", "score": 61.8, "nt": "GCCGAATTGGTTTAGGGGCAAAACAGATCCATGGTAAGGATCAGAGAACAGTTCGACTCTGTTATTCGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 239797, 239799 ], "id": "KMADJIBOEG_33", "locus": "GCA000292445_00444" }, { "type": "cds", "sequence": "contig_1", "start": 239844, "stop": 241589, "strand": "+", "frame": 3, "gene": "parE", "product": "DNA topoisomerase IV subunit B", "db_xrefs": [ "COG:COG0187", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0006265", "GO:0034335", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47445", "UniRef:UniRef90_P47445" ], "nt": "ATGAAAAGTAACTACAGTGCAACTAACATCAAGATCTTAAAGGGTTTGGATGCAGTTAAAAAGCGTCCGGGGATGTACATTGGTTCTACTGATAGTAAGGGTCTGCACCACATGCTATGGGAAATTCTTGCTAACAGTGTTGATGAAGTTTTAGCTGGTTATGCAACCAATATTACTGTTACTTTAGATCTCAACAACACCATTACTGTTAGTGATGATGGCAGGGGTATTCCCTATGAGATCCACCAAGACAGTAACATCTCTACGATCGATACAGTTTTCACCTTTCTCCATGCAGGGGGGAAGTTTGATGATCAGTCATACAAACTAGCAGGGGGATTACATGGGGTTGGTGCATCAGTGGTCAATGCCTTAAGTGATCATTTAGAAGTAACAGTGAAAAGAAATGGTCAGATCTACCAATCAGTTTATCAAGCTGGGGGTAAGATCATCCAAAAAGCCAAAAAGATTGGTGATACAACTAGCCATGGTACCACTGTTAGTTTCCATGCTGACCCTAAGGTCTTTAAAAAGGCTCAATTTGATAGCAACATTATTAAAAGCAGGTTAAAAGAGCTAAGCTTTCTGTTTGCTAAACTAAAGCTCACTTTTACTGATCAAAAAACTAATAAAACCACTGTTTTTTTTAGTACCTCAGGACTAGTTCAGTTCCTTGATGAAATTAATAATACTGTAGAAACACTTGGCCAAAAAACACTGATTAAAGGTGAGAAGGATGGGATTGAAGTGGAAGTGGTTTTCCAGTTTAACCAATCAGATCAAGAGACAATCTTATCATTTGCTAACTCGATTAAAACCTTTGAAGGAGGGAGTCATGAAAATGGGTTTTGTCTTGCCATTAGTGATGTGATCAACAGCTATTGCAGAAAGTACAACTTACTAAAAGAAAAAGATAAAAACTTTCAACTTAGTGAGATCAGACAAGGGTTGAATGCTATTATCAAAGTTAACTTACCTGAAAAAAACATCGCTTTTGAAGGACAAACTAAGAGTAAGTTGTTTTCAAAGGAAGTGAAAAACGTTGTTTATGAATTGGTCCAACAACACTATTTCCAGTTTCTGGAAAGAAACAACAATGATGCTAAATTGATCATTGATAAACTACTCAATGCTAGAAAGATTAAAGAGCAAATCAAACAACAACGTGAGTTGAAAAAAAGTTTATCAAGTCCCCAAAAAGAGAAGATCTTATTTGGAAAGTTAGCACCTTGTCAAACCAAAAAAACCAGTGAAAAAGAGTTGTTTATTGTTGAAGGTGATAGTGCTGGTGGCACTGCTAAAATGGGCCGTGATAGAATTTTTCAAGCTATCTTACCTTTGCGCGGCAAGGTGTTAAATGTTGAAAAAATTAACAATAAGAAGGAAGCGATCACTAACGAAGAGATCCTCACTTTAATCTTTTGTATTGGTACAGGGATTTTAACTAACTTCAACATCAAGGACTTAAAGTACGGAAAGATCATCATTATGACTGATGCAGATAATGATGGCGCACACATCCAAATCCTCTTACTTACCTTCTTTTATAGGTACATGCAACCCCTAATTGAACTGGGCCATGTCTATCTAGCTCTTCCTCCTTTATATAAGCTAGAAACCAAAGATAGAAAAACAGTTAAATACCTCTGGAGTGATTTGGAGTTGGAATCAGTCAAACTAAAGCTTAATAACTTCACTTTACAACGATACAAAGGACTTGGAGAGATGAATGCTGATCAGTTGTGA", "aa": "MKSNYSATNIKILKGLDAVKKRPGMYIGSTDSKGLHHMLWEILANSVDEVLAGYATNITVTLDLNNTITVSDDGRGIPYEIHQDSNISTIDTVFTFLHAGGKFDDQSYKLAGGLHGVGASVVNALSDHLEVTVKRNGQIYQSVYQAGGKIIQKAKKIGDTTSHGTTVSFHADPKVFKKAQFDSNIIKSRLKELSFLFAKLKLTFTDQKTNKTTVFFSTSGLVQFLDEINNTVETLGQKTLIKGEKDGIEVEVVFQFNQSDQETILSFANSIKTFEGGSHENGFCLAISDVINSYCRKYNLLKEKDKNFQLSEIRQGLNAIIKVNLPEKNIAFEGQTKSKLFSKEVKNVVYELVQQHYFQFLERNNNDAKLIIDKLLNARKIKEQIKQQRELKKSLSSPQKEKILFGKLAPCQTKKTSEKELFIVEGDSAGGTAKMGRDRIFQAILPLRGKVLNVEKINNKKEAITNEEILTLIFCIGTGILTNFNIKDLKYGKIIIMTDADNDGAHIQILLLTFFYRYMQPLIELGHVYLALPPLYKLETKDRKTVKYLWSDLELESVKLKLNNFTLQRYKGLGEMNADQL", "aa_hexdigest": "277bb6ee9e7f5a712c22257600721f8c", "start_type": "ATG", "rbs_motif": "ATTTTA", "psc": { "uniref90_id": "UniRef90_P47445", "query_cov": 1.0, "subject_cov": 0.9178515007898894, "identity": 1.0, "score": 1124.0, "evalue": 0.0, "valid": true, "gene": "parE", "product": "DNA topoisomerase IV subunit B", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P47445", "cog_id": "COG0187", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0006265", "GO:0034335", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47445", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47445" ], "product": "DNA topoisomerase 4 subunit B" }, "genes": [ "parE" ], "id": "KMADJIBOEG_480", "locus": "GCA000292445_00445" }, { "type": "cds", "sequence": "contig_1", "start": 241599, "stop": 241745, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCCAACTACCAGAAAGCTAGTGCAAGTAAAGCTTGATGATCTAATTAACGCTGAAAAGCAAATCAACATCTTTATGGGTGAAAAGAGTGATTTGCGCAAACACTGGATTGAAGCCAACATTAACTTTAGTATAGAAAACTAA", "aa": "MNPTTRKLVQVKLDDLINAEKQINIFMGEKSDLRKHWIEANINFSIEN", "aa_hexdigest": "0fb28b9b32d1c2ee5f38341d70b8cb08", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5656.451899999998, "isoelectric_point": 6.539689826965333 }, "id": "KMADJIBOEG_481", "locus": "GCA000292445_00446" }, { "type": "cds", "sequence": "contig_1", "start": 241745, "stop": 242032, "strand": "+", "frame": 2, "gene": null, "product": "DNA gyrase subunit A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9XCU2" ], "nt": "ATGGATCAAAAAAACAACAACCTCTTTCAAAAAGCAATTGAAGAAGTCTTTGCAGTTAGCTTTAGTAAGTATGCTAAATACATCATCCAAGATAGAGCTTTACCTGATCTAAGAGATGGGTTAAAACCAGTGCAAAGACGGATCTTATATGGGATGTTTCAAATGGGCTTAAAACCCACCACTTCCTATAAAAAATCAGCCCGTGCTGTTGGGGAGATCATGGGGAAATACCATCCCCATGGTGATAGTTCCATTTATGATGCAATTATCAGAATGTCCCAAAGCTGA", "aa": "MDQKNNNLFQKAIEEVFAVSFSKYAKYIIQDRALPDLRDGLKPVQRRILYGMFQMGLKPTTSYKKSARAVGEIMGKYHPHGDSSIYDAIIRMSQS", "aa_hexdigest": "27d8d0f5a5e5bc0cc8e2be0b923d5646", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028540D82", "query_cov": 0.8736842105263158, "subject_cov": 0.83, "identity": 0.6629999999999999, "score": 124.0, "evalue": 2.46e-35, "valid": false, "product": "DNA gyrase subunit A", "uniref50_id": "UniRef50_Q9XCU2" }, "pscc": { "uniref50_id": "UniRef50_Q9XCU2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9XCU2" ], "product": "DNA gyrase subunit A" }, "genes": [], "id": "KMADJIBOEG_482", "locus": "GCA000292445_00447" }, { "type": "cds", "sequence": "contig_1", "start": 242078, "stop": 244090, "strand": "+", "frame": 2, "gene": "parC", "product": "DNA topoisomerase IV subunit A", "db_xrefs": [ "COG:COG0188", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0005886", "GO:0006265", "GO:0009330", "GO:0034335", "SO:0001217", "UniRef:UniRef50_P47446", "UniRef:UniRef90_P47446" ], "nt": "GTGGATGGGGATAATGCTGCAGCAATGCGTTACACAGAAACCCGCTTAAGCTTGTATGGATTTGAACTATTAAAAGACATTGATAAAAAGCTAGTTAGTTTTGTTAATAACTTTGATGATAGTGAAAAAGAACCAACGGTTTTACCAACCTTACTGCCTAACCTCTTTATCAATGGTGCGAGTGGGATAGCTGCTGGATATGCAACTAATATTGCTCCCCATAACACTAATGAACTATTAGATAGTCTTTGCTTGCGAATAGATCAACCTGATTGTGAACTTAAACAAATTTTAAAAATTGTTAAAGGTCCTGATTTTCCAACAGGGGGTAATGTTTATTTTGAAAAGAGTTTAAGTGATATTTATCAAGCAGGCAAAGGTAAATTTATTATCCAAGCTAAGTATGAAGTTAACAAGAACTTAAACCAGATTGAAATTACCCAAATCCCTTATGAAACACTGAAAGCTAACATTGTTAAACAAATTGAAGAGATTATCTTTGACAATAAACTCTCTGCTATTGAAAGTGTCATTGATAGTTCAAATCGCAATGGCATTAGGATCATTATTAAACACAAGGACTTTTTGCCTGCTGAGAAGATCATGGCCTTTTTGTTTAAACACACCCAACTCCAAGTGAACTTTAACCTTAATAACACCGTGATTGCTAACCGCTTTCCCATCCAGATTGGTTTACTAAGTTACCTAGATCATTTTTTAAAGTTTGGTCATGAACTAATTATTAATAAAGCTAAGTATGAACTTGAGCTTGCAAGCAAGCGCTTGGAAATTATTTTAGGACTAATTAAAGCGATTAGTATCATTGATAAAATCATCAAATTAATTAGATCAGCAGTTGACAAAAGTGATGCAAGACAAAAGTTAATTGATAACTTTAAATTTACTTTTAACCAATCAGAGGCAATTGTTAGTTTACGACTCTACCAACTAACTAACACTGATATTTTTGAACTTAACCAAGAACAAAATGAACTTGAAAAAACTGTGATTAGTTCAGAGCAACTAATTGCTAGTGAAAAAGCAAGAAACAAACTCCTAAAAAAACAGTTTGAAGGTTATAAAAAGCAGTTTCACCAGCAACGAAGGTCACAAATATGTGGCTTTATTAACCAAAAAAAGGTGGAGGAAAGTGAGCTAATTGAAAACAAAACTTATGGGGTTTTAATCACTAAAGCTGGTAACTACCATAAGTTTGAATCTAACCAACTATTAAAAAGCACCACTGATTTTAAAAGTGAGAGTGACACAATTATCTTTGCACAAACTATTGCTAATACCGACCAAATTTTTATTGTCACTTCACTAGGTAATATTATTAATATCCCTGTTTATAAATTAGCTTTCAATTCCAAAAATAAACTAGCAAGTTTAGTTAGTAAAAAACCAATCCTTTTGGAGTATGAAACGATTGTTTTTGTTGGAACAATGAACAGTGTAAACCAACCAATCCTTGTTTTAACTTCCAAACTAGGAATGGTTAAACGGATTGATTTAACCAAACTTAACATTAAGCCACTTAAAGCTACTTTGTGTATCTCACTCCGTGATAAAGACCATTTAGTAAGTGCATTTTTACAACAAGATGATAAACTGATCTGTTTAGTGTCTGATCACAACTATTACACTGTTTTTCACACCAATGAGATCCCATTAATTAGTAGTAAGGGGATGGGAGTGAAGGGGATGAAGTTAAAACTAGAGGATCAAATTAAGTTTGTTGTTGCTTTTGAAGCTAATGAACCGTTAGTGATGATATGTAGTGATGGTAGTGTCATTAACTTAAAACAAACTGAACTAGTTGTAGTTAGCAGGATGGCAACTGCAAAAAAACTGCCTGTTAAGAAAGCAATTAACTATTGTTTTAGTGATGCAACTAACACCCAGTTAATTAATTTTCAGGGTAAGAACGGTAGTAAATTAATTACAACTAGTGAACTGAACCAGATGAGTAAAACTGCAATTAGTCAAACCAGGTTTAACAAACTTAATTAG", "aa": "MDGDNAAAMRYTETRLSLYGFELLKDIDKKLVSFVNNFDDSEKEPTVLPTLLPNLFINGASGIAAGYATNIAPHNTNELLDSLCLRIDQPDCELKQILKIVKGPDFPTGGNVYFEKSLSDIYQAGKGKFIIQAKYEVNKNLNQIEITQIPYETLKANIVKQIEEIIFDNKLSAIESVIDSSNRNGIRIIIKHKDFLPAEKIMAFLFKHTQLQVNFNLNNTVIANRFPIQIGLLSYLDHFLKFGHELIINKAKYELELASKRLEIILGLIKAISIIDKIIKLIRSAVDKSDARQKLIDNFKFTFNQSEAIVSLRLYQLTNTDIFELNQEQNELEKTVISSEQLIASEKARNKLLKKQFEGYKKQFHQQRRSQICGFINQKKVEESELIENKTYGVLITKAGNYHKFESNQLLKSTTDFKSESDTIIFAQTIANTDQIFIVTSLGNIINIPVYKLAFNSKNKLASLVSKKPILLEYETIVFVGTMNSVNQPILVLTSKLGMVKRIDLTKLNIKPLKATLCISLRDKDHLVSAFLQQDDKLICLVSDHNYYTVFHTNEIPLISSKGMGVKGMKLKLEDQIKFVVAFEANEPLVMICSDGSVINLKQTELVVVSRMATAKKLPVKKAINYCFSDATNTQLINFQGKNGSKLITTSELNQMSKTAISQTRFNKLN", "aa_hexdigest": "c4772e1450382ad08056192d4cb4b721", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47446", "query_cov": 1.0, "subject_cov": 0.8578745198463509, "identity": 0.988, "score": 1239.0, "evalue": 0.0, "valid": true, "gene": "parC", "product": "DNA topoisomerase IV subunit A", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P47446", "cog_id": "COG0188", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0005886", "GO:0006265", "GO:0009330", "GO:0034335" ] }, "pscc": { "uniref50_id": "UniRef50_P47446", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47446" ], "product": "DNA topoisomerase 4 subunit A" }, "genes": [ "parC" ], "id": "KMADJIBOEG_483", "locus": "GCA000292445_00448" }, { "type": "cds", "sequence": "contig_1", "start": 244087, "stop": 244317, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCATTCATAAACCAAACCAACCAAAAGACCAACATTGTCTTTGGTGATCAATTGGGTTTTAAAGAGATATCAGTCGCTTCTACTTTAATCAATACCACCAGTGAAGCCAAGCACCAACTTGCTATAGTAGGACCAACCAGAATGGACTACCAAAAGATTAAAGCCTTATTAACTACTCTAAAAGAAGAGATAGAAAAATATGATAAAAAGATCCACAACCAAACCTAA", "aa": "MAFINQTNQKTNIVFGDQLGFKEISVASTLINTTSEAKHQLAIVGPTRMDYQKIKALLTTLKEEIEKYDKKIHNQT", "aa_hexdigest": "09cf3b39302cca20f7123ef5ddd3eaaa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8638.835499999997, "isoelectric_point": 9.005153465270997 }, "id": "KMADJIBOEG_484", "locus": "GCA000292445_00449" }, { "type": "cds", "sequence": "contig_1", "start": 244324, "stop": 245118, "strand": "-", "frame": 1, "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "db_xrefs": [ "COG:COG1420", "COG:K", "GO:0003677", "GO:0006355", "GO:0045892", "KEGG:K03705", "SO:0001217", "UniRef:UniRef50_P75351", "UniRef:UniRef90_P47447" ], "nt": "ATGAAGAATTTAACGCCACGGCAAGCCCAAATTCTCAAAGCAATTATCAATGAATACATTGCTTATGCTATCCCTGTTGGCAGTAAATTACTAACCAAAAAATATTTTAAAAACCTCTCTGGGGGAACATTACGAAATGAGATGGCAGCTTTAGAGAAAAAAGGGTTCTTAAAGAAAAACCACATCTCTTCAGGCCGTGTTCCTTCCCAAATTGGTTATCAGTATTATGTCAAAGTGTTAAATGTTAGTAACACCACTAACGATCTGAAAACCCGCTTAAGAAGTGTTATTCTCCAGCAACACAAAACGATTGATGAGGTGATTGAACTTGGGGTTAAGTTTATCAATGAGATCATTAACCTACCAGTAGTGTTAACTAACTTTAGTAGTGATGAAGTTTTAAAAAAGATTGATCTGATCATCCTTGATAAGTCATTTGCCTTATTTTTATTGGTCTCCGCTAGTGGTAAAGTCTTTAAAAAAACCATTAGCTATGCTAACCAAAGGCAGTTTGAAGACATTGTTATTTGTGTAAGGATCTTTAATGATCGCATCATTGATACCCGCTTTTCAGAGATTAACAACCAACTAGAAGTTTTAAAAGAGATCATTAGAACCAAGGTTCATGAGTATCAGTATGTAATTGATGAGATCTTATTTAAGTTATTTGATTTAGATCAAATTGAGGCAAATAAAAAGATCTATGGGATCCAATACCTTGCTAAACAACCCGAGTTTGCTAACCAAGAGAAACTCACTAAAATCTTAAATCTGCTTGAAGATACTAGTGTTTGA", "aa": "MKNLTPRQAQILKAIINEYIAYAIPVGSKLLTKKYFKNLSGGTLRNEMAALEKKGFLKKNHISSGRVPSQIGYQYYVKVLNVSNTTNDLKTRLRSVILQQHKTIDEVIELGVKFINEIINLPVVLTNFSSDEVLKKIDLIILDKSFALFLLVSASGKVFKKTISYANQRQFEDIVICVRIFNDRIIDTRFSEINNQLEVLKEIIRTKVHEYQYVIDEILFKLFDLDQIEANKKIYGIQYLAKQPEFANQEKLTKILNLLEDTSV", "aa_hexdigest": "681cc5b1d1a7ea27d7e8294c69d49085", "start_type": "ATG", "rbs_motif": null, "genes": [ "hrcA" ], "truncated": "5-prime", "pseudogene": { "start": 244087, "stop": 245118, "observations": { "mutation": [ 244326 ], "stop": [ 244326 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47447", "query_cov": 1.0, "subject_cov": 0.7696793002915452, "identity": 1.0, "score": 503.0, "evalue": 1.68e-178, "gene_start": 1, "gene_end": 264, "reference_start": 1, "reference_end": 264, "reference_sequence": "MKNLTPRQAQILKAIINEYIAYAIPVGSKLLTKKYFKNLSGGTLRNEMAALEKKGFLKKNHISSGRVPSQIGYQYYVKVLNVSNTTNDLKTRLRSVILQQHKTIDEVIELGVKFINEIINLPVVLTNFSSDEVLKKIDLIILDKSFALFLLVSASGKVFKKTISYANQRQFEDIVICVRIFNDRIIDTRFSEINNQLEVLKEIIRTKVHEYQYVIDEILFKLFDLDQIEANKKIYGIQYLAKQPEFANQEKLTKILNLLEDTSVWQQMAFINQTNQKTNIVFGDQLGFKEISVASTLINTTSEAKHQLAIVGPTRMDYQKIKALLTTLKEEIEKYDKKIHNQT" }, "paralog": false, "identity": 0.9970845481049563, "score": 657.0, "evalue": 6.74e-242, "description": "Internal stop codon at 244326. Nonsense mutation around 244326.", "psc": { "uniref90_id": "UniRef90_P47447", "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "uniref50_id": "UniRef50_P75351", "cog_id": "COG1420", "cog_category": "K", "kegg_orthology_id": "K03705", "go_ids": [ "GO:0003677", "GO:0006355", "GO:0045892" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47447", "UniRef:UniRef50_P75351", "COG:COG1420", "COG:K", "KEGG:K03705", "GO:0003677", "GO:0006355", "GO:0045892" ] }, "pscc": { "uniref50_id": "UniRef50_P75351", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75351" ], "product": "Heat-inducible transcription repressor HrcA" } }, "id": "KMADJIBOEG_485", "locus": "GCA000292445_00450" }, { "type": "cds", "sequence": "contig_1", "start": 245487, "stop": 246947, "strand": "+", "frame": 3, "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "db_xrefs": [ "COG:COG0322", "COG:L", "GO:0003677", "GO:0005737", "GO:0006289", "GO:0006974", "GO:0009380", "GO:0009381", "GO:0009432", "KEGG:K03703", "SO:0001217", "UniRef:UniRef50_P47448", "UniRef:UniRef90_P47448" ], "nt": "TTGGAATTAGCCAGAAAATACCAGATTAAAACCACTAAGTGCTTTGGACCGTTTGCTTCAAGTAAGTTTAAGTTACGTGAGATCCATGACCTACTTTTAAAACTCTTTCCTTTAAGAAAGTGTGCACCTCACCAAAAAAACCACCCGTGCTTTTATTTTCAGATGGGTTTATGTATGGGGCAATGTATGCAAACTGATACTAAGGAAAAATACCAACAGGTAATTAGTAACATTGAACAGTTTTTTAATGACCCTAGTGTGGTAATTAACTATTTAAAAGCTGCAGAAAAAAAGGCAAGTGATAATCAGGAATTTGAAAAGGCCCAGCAGTTTCTAACACTGCAAAAAGCAGTTTTAGAGTTAACAAAAACCCACCATACCACTATCATTAAACAAAAATCAAGCCATGATTTTATTGGGTATGTCTTTCAAAATAACGTTTTGGCCATTACCATTTTTTGTTATGAAAAAGGGGAGTTAACTGATAAAGAACAAGCAGTGTTTACCCTAGAGCAAACTGACATTGTGGAAGTTGAAAGTGCTATTATCACCTTTATCTACCACCACTATAAAACTACCCCACTTCCAAGTAAGATTACTGTTTCACTTGATGAAACTAACCTAAAACTTATTAGTGATAGCTTAAAAATTGGTGTTTTTAAGCCCAAGGATGGTAATGAAAAACTGATCTTACAAACTGTTATTGATAATGCCAAACATGCACTTGCAACCAAGTGGTTGAAGTTTACTAGTAACTATGATAAAACCCGGCTCCACAAGGATTTAGCACAACTTCTAAATACTGATTATATCCATAGTCTTGAGATTATTGATGTGTCATTCTATGATCAAAACCATGTTGTCGGTTGCATGTTAAGGTTTGAAGATGGTAAAAAGATCAAACACTTATCAAGAAGATACAACATTAACAGTTTAAAAAAAGGTGATACTAACCACATTGCTTTACTTGTTTACAGAAGGATCTTAAGTGCGATGCAAACCAAAGCTAACCTCCCTTTTAGTGATCTTTTAATTATTGATGGTGGTAAAGCACAAATTAAAAGTGTTAAGCAAGTTTTTAGTCTCTTCAGTAATGTTAAACCACCCATTATCATTGGACTAGTTAAAAACAAAAACCACCAAACTGATCACATTATGTTATCTGATTTCCAAGTTAAAAAGATAGCAATTAACTCCCCACTCTTTCACTATTTAGCAACAATCCAAACTGAAGTTGATGGTTTTGCTAAAAGAAGTGCTTTTAATAAGTTAAGTAACCACCAACTGCAAAACCCGTTGCTACAAATCCCAGGAGTTGGCAAGATAACTGCCCAAATTCTCTTTGATAACTTTCAAACGCTCAATAACATAAAATTAGCTTCAGTTAATGAGTTAAGCCAGTTTATTAAAAAACCATTAGCACAAAAGATTAAAACTTACTTTGCAAAACAAACTGATTAA", "aa": "MELARKYQIKTTKCFGPFASSKFKLREIHDLLLKLFPLRKCAPHQKNHPCFYFQMGLCMGQCMQTDTKEKYQQVISNIEQFFNDPSVVINYLKAAEKKASDNQEFEKAQQFLTLQKAVLELTKTHHTTIIKQKSSHDFIGYVFQNNVLAITIFCYEKGELTDKEQAVFTLEQTDIVEVESAIITFIYHHYKTTPLPSKITVSLDETNLKLISDSLKIGVFKPKDGNEKLILQTVIDNAKHALATKWLKFTSNYDKTRLHKDLAQLLNTDYIHSLEIIDVSFYDQNHVVGCMLRFEDGKKIKHLSRRYNINSLKKGDTNHIALLVYRRILSAMQTKANLPFSDLLIIDGGKAQIKSVKQVFSLFSNVKPPIIIGLVKNKNHQTDHIMLSDFQVKKIAINSPLFHYLATIQTEVDGFAKRSAFNKLSNHQLQNPLLQIPGVGKITAQILFDNFQTLNNIKLASVNELSQFIKKPLAQKIKTYFAKQTD", "aa_hexdigest": "e07f7880b4e28f58bd03eadd817256db", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47448", "query_cov": 1.0, "subject_cov": 0.8140703517587939, "identity": 0.9940000000000001, "score": 955.0, "evalue": 0.0, "valid": true, "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "uniref50_id": "UniRef50_P47448", "cog_id": "COG0322", "cog_category": "L", "kegg_orthology_id": "K03703", "go_ids": [ "GO:0003677", "GO:0005737", "GO:0006289", "GO:0006974", "GO:0009380", "GO:0009381", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P47448", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47448" ], "product": "UvrABC system protein C" }, "genes": [ "uvrC" ], "id": "KMADJIBOEG_486", "locus": "GCA000292445_00451" }, { "type": "cds", "sequence": "contig_1", "start": 246928, "stop": 247086, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00149.33" ], "nt": "TTGCAAAACAAACTGATTAAGGTTTTGGTAATTGCTGATACCCATGGTCAAAACCAGAGGTGGATTGAACTGAAAAACTACCATAACCCTGATGTGATTATCCATGCAGGAGATCACATGACCACTAAACAATTCATGGATCAAAATGCTACTTTTTGA", "aa": "MQNKLIKVLVIADTHGQNQRWIELKNYHNPDVIIHAGDHMTTKQFMDQNATF", "aa_hexdigest": "de0b7a5eb7188aeeb12b65db36c68253", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00149.33", "name": "Metallophos", "length": 42, "aa_cov": 0.6923076923076923, "hmm_cov": 1.0, "evalue": 5.307038149604768e-08, "score": 28.64311981201172, "start": 6, "stop": 41 } ], "seq_stats": { "molecular_weight": 6104.909600000002, "isoelectric_point": 6.894762992858888 }, "id": "KMADJIBOEG_487", "locus": "GCA000292445_00452" }, { "type": "cds", "sequence": "contig_1", "start": 247087, "stop": 247431, "strand": "+", "frame": 1, "gene": "yfcE", "product": "putative metallophosphoesterase MG207", "db_xrefs": [ "COG:COG0622", "COG:R", "EC:3.1.4.-", "GO:0016787", "GO:0046872", "KEGG:K07095", "SO:0001217", "UniRef:UniRef50_P47449", "UniRef:UniRef90_P47449" ], "nt": "GTGGCAGGTAACAACGATTCAATTGGCAATGAAATTGAAATTTTCCAGTTAGGGCAAATTAACTTTGTGTTAATGCATGGTCACCAAGCACCAAGAGATAACCTGAAAAAGTGGTACCAATTATTAGTTTTAAAAGCACAACAATACCCTTGTGATGTTTTAATCTTTGGTCATAGTCACATTGAATATACCAACAAAATTAATAAGATCCAGTTAATCAACCCTGGTTCTCTACAACTACCAAGAAACCAAACCAACACCCCTTCATACTGTACCTTTATTGTCAATAAAGACGAGCTAACTGATCTAACTATCCACTATTACCAAGCTTCAAAAGTTAGTTAA", "aa": "MAGNNDSIGNEIEIFQLGQINFVLMHGHQAPRDNLKKWYQLLVLKAQQYPCDVLIFGHSHIEYTNKINKIQLINPGSLQLPRNQTNTPSYCTFIVNKDELTDLTIHYYQASKVS", "aa_hexdigest": "ed7f234b411cb1db7364d7bed4f50ba6", "start_type": "GTG", "rbs_motif": null, "genes": [ "yfcE" ], "truncated": "5-prime", "pseudogene": { "start": 246940, "stop": 247431, "observations": { "mutation": [ 247084 ], "stop": [ 247084 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47449", "query_cov": 1.0, "subject_cov": 0.6993865030674846, "identity": 0.982, "score": 235.0, "evalue": 5.13e-78, "gene_start": 1, "gene_end": 114, "reference_start": 50, "reference_end": 163, "reference_sequence": "MIKVLVIADTHGQNQRWIELKNYHNPDVIIHAGDHMTTKQFMDQNATFWVAGNNDSIGNEIEIFQLGQINFVLMHGHQAPRDNLKKWYQLLVLKAQQYPCDVLIFGHSHIEYTNKINMIQLINPGSLQLPRNQTNTPSYCTFIVNKDELTDLTIHYYQASKVS" }, "paralog": false, "identity": 0.9815950920245399, "score": 334.0, "evalue": 2.25e-119, "description": "Internal stop codon at 247084. Nonsense mutation around 247084.", "psc": { "uniref90_id": "UniRef90_P47449", "gene": "YfcE", "product": "Probable metallophosphoesterase MG207", "ec_ids": [ "3.1.4.-" ], "uniref50_id": "UniRef50_P47449", "cog_id": "COG0622", "cog_category": "R", "kegg_orthology_id": "K07095", "go_ids": [ "GO:0016787", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47449", "EC:3.1.4.-", "UniRef:UniRef50_P47449", "COG:COG0622", "COG:R", "KEGG:K07095", "GO:0016787", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47449", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47449" ], "product": "Probable metallophosphoesterase MG207" } }, "id": "KMADJIBOEG_488", "locus": "GCA000292445_00453" }, { "type": "cds", "sequence": "contig_1", "start": 247428, "stop": 248015, "strand": "-", "frame": 2, "gene": "tsaB", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB", "db_xrefs": [ "COG:COG1214", "COG:J", "EC:2.3.1.234", "GO:0002949", "RefSeq:WP_014894215.1", "SO:0001217", "UniParc:UPI00027B3BD5", "UniRef:UniRef100_UPI00027B3BD5", "UniRef:UniRef50_P47450", "UniRef:UniRef90_P47450" ], "nt": "ATGTTCTTTTTAAGTAAGTATAAGCTGTTTTTAGACTGTGCTTATAAAACCCTTAACATCATTATTTTAGAGATGAAAACCAATGCAGTGGTTGATGAGTTGTCCATTGGTGTTGAACAGAACTTAACTGAACTTGCTGTTTATTATCTGGAGACAATGTTAACAAAAAACAAGTTGAAAAAAAGCAGTATTAAACAGTTTTATGTCACAATTGGCCCTGGTAGTTTCACCGGTCAGAGGATAGCAACTATCATTGCTAAAAGCTGGTGTTTATTGTATCCCAGTTGTGAACTTTATGCTTTAAATTCACTCCGCTTTCAAATCCCATATGAACATGGGATTAGCAAGATTAGTTGTGGTAATGATCAGAACTATTGTGGACTATACAGCCAAACTACTAGTGAAATAAAGCTAATTAGTAAAGCTGATTTTGTGAAGTTATGTAAGGCAAACAATGAACTTCCTATGTATGAAAACTTTGAAAACATTGAGAGTTATAGCAAGCTGTTATTAAGCAACATCGATCACTTTGAAAGAATCGAAGATCCTCTAACCTTACAACCTATCTATCTAAAAGATCCCTGTTAA", "aa": "MFFLSKYKLFLDCAYKTLNIIILEMKTNAVVDELSIGVEQNLTELAVYYLETMLTKNKLKKSSIKQFYVTIGPGSFTGQRIATIIAKSWCLLYPSCELYALNSLRFQIPYEHGISKISCGNDQNYCGLYSQTTSEIKLISKADFVKLCKANNELPMYENFENIESYSKLLLSNIDHFERIEDPLTLQPIYLKDPC", "aa_hexdigest": "afb4614199425217889f7a80f70ee70f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3BD5", "ncbi_nrp_id": "WP_014894215.1", "uniref100_id": "UniRef100_UPI00027B3BD5", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3BD5", "RefSeq:WP_014894215.1", "UniRef:UniRef100_UPI00027B3BD5" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3BD5", "uniref90_id": "UniRef90_P47450" }, "psc": { "uniref90_id": "UniRef90_P47450", "gene": "tsaB", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_P47450", "cog_id": "COG1214", "cog_category": "J", "go_ids": [ "GO:0002949" ] }, "pscc": { "uniref50_id": "UniRef50_P47450", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47450" ], "product": "Uncharacterized protein MG208" }, "genes": [ "tsaB" ], "id": "KMADJIBOEG_489", "locus": "GCA000292445_00454" }, { "type": "cds", "sequence": "contig_1", "start": 247999, "stop": 248925, "strand": "-", "frame": 1, "gene": "rluA", "product": "putative RNA pseudouridine synthase MG209", "db_xrefs": [ "COG:COG0564", "COG:J", "EC:5.4.99.-", "GO:0000455", "GO:0003723", "GO:0009982", "GO:0120159", "KEGG:K06180", "RefSeq:WP_009885746.1", "SO:0001217", "UniParc:UPI0000139478", "UniRef:UniRef100_P47451", "UniRef:UniRef50_P47451", "UniRef:UniRef90_P47451" ], "nt": "ATGAAACAGTGTTTTGTTGTTACAACTACCAAACGCTTAGATAGTCTTTTAGCTAGCTTACTGAACCTTTCAAGAGTAAAGGTAGTGAAGCTGATCATGAATGGACAGATTAAAGTTAATGAAAAACTAACTTTTAAAAACAGTTTAATAGTTGCAAAAGATGATGTAATTAAAGTTGAGATTCATGATGAGACAACTAGTGATTTCATTACTAGTGTTGAACCTTATAACTTAAAGCTTGAGGTTCTTTTTGAAGACAAGGATTTGATGGTTATTAACAAACCATCAGGTTTGTTAACCCATCCCACCACTTTCAATGAAAAAGCCAGCTTGTTAGCTGCTTGTATCTTTCACAACAACAAAAACCCTGTTTACTTAGTGCACAGATTGGACCGTGATACTAGTGGGGCAATTGTTGTCTGTAAAAACCAAGCAAGCTTATTAAATTTGCAAAATCAACTGCAAAATCGCACCTTAAAACGTTATTATGTAGCACTAGTCCACTTCCCTTTTAATGCCTTAACTGGTTCAATTAATGCACCTTTAGCAAGGGTTAATAACAACAAGGTAATGTTTAAAATAGCCCAAACTGCTAAAGCAAAGCAAGCAATAACTAAGTTTAAAGTGATTAATCAGAATGAAAAAGCAGCACTAATTAGCTTGGAATTGTTAACAGGTAGAACCCACCAAATTAGAGTGCATCTGAAATTTATCCAACATCCAGTTTATAATGATCCACTGTATGGAATTAAAAGTGAGAAGAAAGATAGCTATGGTCAGTTTCTCCATGCAAACAGGATCTGTTTTATCCATCCCACTTTAAACAAACCTATGGACTTTCACGCCCCACTTGAACCTAAGTTTTCAACGAAACTTAAGAGTTTAAACTTATCTTTAACCGATCCACTCCATGTTCTTTTTAAGTAA", "aa": "MKQCFVVTTTKRLDSLLASLLNLSRVKVVKLIMNGQIKVNEKLTFKNSLIVAKDDVIKVEIHDETTSDFITSVEPYNLKLEVLFEDKDLMVINKPSGLLTHPTTFNEKASLLAACIFHNNKNPVYLVHRLDRDTSGAIVVCKNQASLLNLQNQLQNRTLKRYYVALVHFPFNALTGSINAPLARVNNNKVMFKIAQTAKAKQAITKFKVINQNEKAALISLELLTGRTHQIRVHLKFIQHPVYNDPLYGIKSEKKDSYGQFLHANRICFIHPTLNKPMDFHAPLEPKFSTKLKSLNLSLTDPLHVLFK", "aa_hexdigest": "07f74059485ad672f0972c9e1a18256b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139478", "ncbi_nrp_id": "WP_009885746.1", "uniref100_id": "UniRef100_P47451", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139478", "RefSeq:WP_009885746.1", "UniRef:UniRef100_P47451" ] }, "ips": { "uniref100_id": "UniRef100_P47451", "uniref90_id": "UniRef90_P47451" }, "psc": { "uniref90_id": "UniRef90_P47451", "gene": "RluA", "product": "Uncharacterized RNA pseudouridine synthase MG209", "ec_ids": [ "5.4.99.-" ], "uniref50_id": "UniRef50_P47451", "cog_id": "COG0564", "cog_category": "J", "kegg_orthology_id": "K06180", "go_ids": [ "GO:0009982", "GO:0003723", "GO:0120159", "GO:0000455" ] }, "pscc": { "uniref50_id": "UniRef50_P47451", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47451" ], "product": "Uncharacterized RNA pseudouridine synthase MG209" }, "genes": [ "rluA" ], "id": "KMADJIBOEG_490", "locus": "GCA000292445_00455" }, { "type": "cds", "sequence": "contig_1", "start": 249131, "stop": 249469, "strand": "-", "frame": 3, "gene": "lspA", "product": "signal peptidase II", "db_xrefs": [ "COG:COG0597", "COG:MU", "EC:3.4.23.36", "GO:0004190", "GO:0005886", "GO:0006508", "GO:0009306", "KEGG:K03101", "SO:0001217", "UniRef:UniRef50_P75484", "UniRef:UniRef90_Q49401" ], "nt": "ATGAAATTAAGAAAAACCAAGTTTTTTTCACAACTTAAACACCAGGTTTTAACTGCAAACCAAAAACCATTTTTATTCTATAAACTGACAATGATTGGGTTTGTTGGCTTTATTATCTTACTGCAAGTTTTCATATTAAAAAATGCGTTAAATGGTGAGATGGATAACACCATGGTAGCAAATAGTGGTTTTATTAATATCTATGTGATTAGAAACAAAGGGGTAGGGTTTAGCTTATTACAAAACCAAACTGGCTTAGTTTACTTTCTCCAGGGATTATTATCAGTAATTGCGTTAGTTTTTCTTGTTTTTATGGTGAAATATAGTTACATCTTTTGA", "aa": "MKLRKTKFFSQLKHQVLTANQKPFLFYKLTMIGFVGFIILLQVFILKNALNGEMDNTMVANSGFINIYVIRNKGVGFSLLQNQTGLVYFLQGLLSVIALVFLVFMVKYSYIF", "aa_hexdigest": "5550ef0f4009cc0cd8ca4f2f2d6ad1bc", "start_type": "ATG", "rbs_motif": null, "genes": [ "lspA" ], "truncated": "5-prime", "pseudogene": { "start": 248925, "stop": 249469, "observations": { "deletion": [ 248971 ], "mutation": [ 249133 ], "stop": [ 249133 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49401", "query_cov": 1.0, "subject_cov": 0.6187845303867403, "identity": 0.991, "score": 206.0, "evalue": 4.6e-66, "gene_start": 1, "gene_end": 112, "reference_start": 1, "reference_end": 112, "reference_sequence": "MKLRKTKFFSQLKHQVLTANQKPFLFYKLTMIGFVGFIILLQVFILRNALNGEMDNTMVANSGFINIYVIRNKGVGFSLLQNQTGLVYFLQGLLSVIALVFLVFMVKYSYIFWITTLAFGSLGNFFDRLTSANDSVLDYFIFQNGSSVFNFADCCITFGFIGLFFCFLIQMFKEFKHSKNQ" }, "paralog": false, "identity": 0.988950276243094, "score": 351.0, "evalue": 7.95e-126, "description": "Internal stop codon at 249133. Frameshift due to deletion around 248971. Nonsense mutation around 249133.", "psc": { "uniref90_id": "UniRef90_Q49401", "gene": "lspA", "product": "signal peptidase II", "ec_ids": [ "3.4.23.36" ], "uniref50_id": "UniRef50_P75484", "cog_id": "COG0597", "cog_category": "MU", "kegg_orthology_id": "K03101", "go_ids": [ "GO:0004190", "GO:0005886", "GO:0006508", "GO:0009306" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49401", "EC:3.4.23.36", "UniRef:UniRef50_P75484", "COG:COG0597", "COG:MU", "KEGG:K03101", "GO:0004190", "GO:0005886", "GO:0006508", "GO:0009306" ] }, "pscc": { "uniref50_id": "UniRef50_P75484", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75484" ], "product": "Lipoprotein signal peptidase" } }, "id": "KMADJIBOEG_491", "locus": "GCA000292445_00456" }, { "type": "cds", "sequence": "contig_1", "start": 250072, "stop": 250317, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTTAGTTGGTTTACCCTTTGAAAAAGGTGCTTATCATAAGCAGTTATATGACCATTCACGTCAGGAGATCTTAGATCTTACTGAAGTTATTAAAAAGCTTTATCTAAAAACAGAGACCAACAACGATAAGCTTGAGTTTGCTGCAACTACTAGTAAAACAACAGCGCAGCTAACTAAAACTATGCCCTTAAACAGTAGTGATCTTGATAAGGATCTAATGGAGTTTAGTGGTGAGAAGTGA", "aa": "MILVGLPFEKGAYHKQLYDHSRQEILDLTEVIKKLYLKTETNNDKLEFAATTSKTTAQLTKTMPLNSSDLDKDLMEFSGEK", "aa_hexdigest": "9b80defb3d37c571d723cb3422c4fe48", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9266.492899999997, "isoelectric_point": 5.603665351867675 }, "id": "KMADJIBOEG_492", "locus": "GCA000292445_00457" }, { "type": "cds", "sequence": "contig_1", "start": 250329, "stop": 250517, "strand": "+", "frame": 3, "gene": "rpsU", "product": "30S ribosomal protein S21", "db_xrefs": [ "COG:COG0828", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_009885749.1", "SO:0001217", "UniParc:UPI0000134E7C", "UniRef:UniRef100_P57085", "UniRef:UniRef50_Q8EUK4", "UniRef:UniRef90_P57079" ], "nt": "GTGCCTAAGATTGAAGTTAAAAATGATGATTTAGAGCTAGCTTTAAAAAAGTTTAAAAGGATATCACTGGAAGTACGCAGGTTAGCACAACGCCATGAATACCACTTGCGCAAAGGGATGCGGTTAAGAGAAAAACAAAAGATAGCACAAAAAAAGCGCAGGAAGTTTCGCAGTTTAGCTAGCCATTAA", "aa": "MPKIEVKNDDLELALKKFKRISLEVRRLAQRHEYHLRKGMRLREKQKIAQKKRRKFRSLASH", "aa_hexdigest": "9ff4ed1fff707dabf05dea47bbb375a5", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134E7C", "ncbi_nrp_id": "WP_009885749.1", "uniref100_id": "UniRef100_P57085", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134E7C", "RefSeq:WP_009885749.1", "UniRef:UniRef100_P57085" ] }, "ips": { "uniref100_id": "UniRef100_P57085", "uniref90_id": "UniRef90_P57079" }, "psc": { "uniref90_id": "UniRef90_P57079", "gene": "rpsU", "product": "30S ribosomal protein S21", "uniref50_id": "UniRef50_Q8EUK4", "cog_id": "COG0828", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q8EUK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8EUK4" ], "product": "Small ribosomal subunit protein bS21" }, "genes": [ "rpsU" ], "id": "KMADJIBOEG_493", "locus": "GCA000292445_00458" }, { "type": "cds", "sequence": "contig_1", "start": 250519, "stop": 250917, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MG211", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47453", "UniRef:UniRef90_P47453" ], "nt": "ATGGATAATAAAAACCCCCAGAAACTTATTACTAGTGAATTGTTGGCAAACCACCGCTTTAATTTTGCTAAAGATGATAAAGGTGGTTATGATGCTAATGAAGTTGATGCATTCTTAGACCAGCTAACCAAGACTTTAATCCACTATGAGGAGATGAAAAACAACGAACAGGAGTTGAAAAATGCTTACGACAAGTTGTTTTCAGATCGTGATCAGATTTTAAGTCGTTGTGCTAAATTAGAAGCTGATTTAAACACCTTTTATGAAAATGGTTATGCAAACAAGGTGCTAATTAACCGGGTTCAGGAGTTGGAGGATAAACTTGAAAAACTACCTGATCGTTACACTGAAAAACTAGAAAGGATTGAAAAACTGTTAAAAAAGGTCATTAAACACTGA", "aa": "MDNKNPQKLITSELLANHRFNFAKDDKGGYDANEVDAFLDQLTKTLIHYEEMKNNEQELKNAYDKLFSDRDQILSRCAKLEADLNTFYENGYANKVLINRVQELEDKLEKLPDRYTEKLERIEKLLKKVIKH", "aa_hexdigest": "e87fa732e2b19cb9f0e9d399a42f753a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47453", "query_cov": 1.0, "subject_cov": 0.8979591836734694, "identity": 1.0, "score": 258.0, "evalue": 6.36e-87, "valid": true, "product": "Uncharacterized protein MG211", "uniref50_id": "UniRef50_P47453" }, "pscc": { "uniref50_id": "UniRef50_P47453", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47453" ], "product": "Uncharacterized protein MG211" }, "genes": [], "id": "KMADJIBOEG_494", "locus": "GCA000292445_00459" }, { "type": "cds", "sequence": "contig_1", "start": 250965, "stop": 251081, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGTTGGGATAGGGATTGATGTTGTGCAATTAAAGCGCTTCTTAACTTTAGTTGAAACTAGTGATTGTTTTGCTAAACGATTGTTAACTAGCAATGAACTAAACAGTTATTGA", "aa": "MVVGIGIDVVQLKRFLTLVETSDCFAKRLLTSNELNSY", "aa_hexdigest": "a13dc5c3893cda2b5abaf11f801c8775", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4273.9681, "isoelectric_point": 5.968969535827637 }, "id": "KMADJIBOEG_495", "locus": "GCA000292445_00460" }, { "type": "cds", "sequence": "contig_1", "start": 251302, "stop": 251496, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAACTATTTAAAACAAGTTTTAGATTCATAATAAGGTTTTTACAAATCCTGAGTTTACCAGTTGTTTTTCCTTACTTTTTATTAAGCTTTTTAGCTTGTTTAATTACTAGTAAAAACTATGAATCACTCCCTGACAACTATCCCCCTGAAATCCGGTTCAAAAAGGTGTATAGATTGGTATCAATGTGA", "aa": "MDKLFKTSFRFIIRFLQILSLPVVFPYFLLSFLACLITSKNYESLPDNYPPEIRFKKVYRLVSM", "aa_hexdigest": "fae7e1aad6f1b89ec1228e0559e34c3f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7699.1884, "isoelectric_point": 9.728617668151855 }, "id": "KMADJIBOEG_496", "locus": "GCA000292445_00461" }, { "type": "cds", "sequence": "contig_1", "start": 251521, "stop": 252108, "strand": "+", "frame": 1, "gene": "plsC", "product": "putative 1-acyl-sn-glycerol-3-phosphate acyltransferase", "db_xrefs": [ "COG:COG0204", "COG:I", "EC:2.3.1.51", "GO:0003841", "GO:0006654", "GO:0016020", "GO:0016024", "SO:0001217", "UniRef:UniRef50_Q49402", "UniRef:UniRef90_Q49402" ], "nt": "GTGACAGTAAATGACAAGATTATCCCTAAAAAACCAGTTTTAGTGGTAGCTAACCATAAATCTAACCTTGATCCTTTAGTATTAATTAAGGCCTTTGGTAAGTTGAAAAATAGTCCACCATTAACCTTTGTTGCTAAGATTGAACTGAAAGATACAGTCCTTTTTAAACTGATGAAATTAATTGATTGTGTTTTTATTGATCGAAAAAACATCAGACAAATTGCCAATGCATTGGAAACCCAACAACAACTAATTCGCAAGGGCACTGCTATTGCTGTTTTTGCTGAAGGGACTAGGATTTTAAGTAATGACATTGGAGAATTTAAACCAGGAGCACTAAAGGTTGCTTACAATGCTTTTGTACCTATCTTACCAGTTAGTATTGTGGGTAGCTTAGGAAAGATGGAATCAAACAAAAGGCTAAAAGAACATGGTGTTAAGAAAAATTCAAACTATGAGGTTAAAGTAATCTTTAACAAGCTAATTAACCCAATTAGTTTTAACCAGATTGATTCTAATAACCTTGCTAATAACATTAGAAGCATTATTAGTGATGCATACACTAGTGAAAAACCAAGCAATGATTAG", "aa": "MTVNDKIIPKKPVLVVANHKSNLDPLVLIKAFGKLKNSPPLTFVAKIELKDTVLFKLMKLIDCVFIDRKNIRQIANALETQQQLIRKGTAIAVFAEGTRILSNDIGEFKPGALKVAYNAFVPILPVSIVGSLGKMESNKRLKEHGVKKNSNYEVKVIFNKLINPISFNQIDSNNLANNIRSIISDAYTSEKPSND", "aa_hexdigest": "4e5730143841dc074edc5008f3945fcc", "start_type": "GTG", "rbs_motif": null, "genes": [ "plsC" ], "truncated": "5-prime", "pseudogene": { "start": 251302, "stop": 252108, "observations": { "mutation": [ 251494 ], "stop": [ 251494 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49402", "query_cov": 1.0, "subject_cov": 0.7276119402985075, "identity": 0.9790000000000001, "score": 364.0, "evalue": 6.79e-126, "gene_start": 1, "gene_end": 195, "reference_start": 74, "reference_end": 268, "reference_sequence": "MDKLFKTSFRFIIRFLQILSLPVVFPYFLLSFLACLITSKNYESLPYNYPPEIRFKKVYRLVSMWLYIKGIKVVTVNDKIIPKKPVLVVANHKSNLDPLVLIKAFGRLKNSPPLTFVAKIELKDTVLFKLMKLIDCVFIDRKNIRQIANALETQQQLIRQGTAIAVFAEGTRILSNDIGEFKPGALKVAYNAFVPILPVSIVGSLGKMESNKRLKEHGVKKSSNYEVKVIFNKLINPISFNQIDSNNLANNIRSIISDAYTSEKPSND" }, "paralog": false, "identity": 0.9813432835820896, "score": 511.0, "evalue": 2.96e-186, "description": "Internal stop codon at 251494. Nonsense mutation around 251494.", "psc": { "uniref90_id": "UniRef90_Q49402", "gene": "plsC", "product": "Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase", "ec_ids": [ "2.3.1.51" ], "uniref50_id": "UniRef50_Q49402", "cog_id": "COG0204", "cog_category": "I", "go_ids": [ "GO:0016020", "GO:0003841", "GO:0016024", "GO:0006654" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49402", "EC:2.3.1.51", "UniRef:UniRef50_Q49402", "COG:COG0204", "COG:I", "GO:0016020", "GO:0003841", "GO:0016024", "GO:0006654" ] }, "pscc": { "uniref50_id": "UniRef50_Q49402", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49402" ], "product": "Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase" } }, "id": "KMADJIBOEG_497", "locus": "GCA000292445_00462" }, { "type": "cds", "sequence": "contig_1", "start": 252275, "stop": 252463, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAACCGAATTAACCCCAAATTCAAAACTCACAGTTTTTAACAACTACCAGCAACTTTTAGTTGATAATAATGACCTAATTATCTTTGCAACCCCCACTTTAGTTCAGCTTTTTGATAATGAGATTGACCAGTTAATAGTGATCAATCCCACTAGTAAATCTAAAGATCAGTTTAACTGTAATTGA", "aa": "MQTELTPNSKLTVFNNYQQLLVDNNDLIIFATPTLVQLFDNEIDQLIVINPTSKSKDQFNCN", "aa_hexdigest": "ada697e9a5f1dabb980c0eb2747ea4bf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7115.954700000001, "isoelectric_point": 4.089929389953612 }, "id": "KMADJIBOEG_498", "locus": "GCA000292445_00463" }, { "type": "cds", "sequence": "contig_1", "start": 252632, "stop": 253516, "strand": "+", "frame": 2, "gene": null, "product": "segregation/condensation protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF5CFDF" ], "nt": "GTGGATATTGGCTTGATTTCCTTACAATATCTCAATATCATTGCAAATTACACCAATAAAAAAGCCATTGAAAAGATCACTGATTATCTGGTGATTACAAGCAAAATTCTCGCTAAAAAAGCAGATAATCTGCTCAATGATCATCAGGAAGAGAGTGTGTTGGAATATGATTTAGCTACTAATAACTTTCGTGATAAGATGATCGCTAACTTAGTGGAACACAAACGCTATTGTGATTCACTGGGTGAATTTGAAAAACTCAGGGTTAACCGCTTAGCTTACTTTTCCAAAGCTAATGAGATGGAGCAATTTATCAAAACAGCTAATGATCAACTTGTTACAGTTGAAGACCAACTCCCTAACTATATTAGTGTCTTAAAACTCTTTCATGCTATGAACAAGTTACTGGAGATGAGACTCAGCTCTCTTCTAACAAATAAAAACATCACCATTAAAGAGTTATCAGTTGAACAGGTTCAAAAGGAGTTGGTTTTAGCAATCAAACAGTTTAACTACCAAACAGTTTCTTTAAAGCGGGTGTTATTAAAACTTAACCATCCTATCTCTTTAATGTATTTTGTTACTGCTTTTGTGGCACTTTTAGTGCTATTAAACAACCAGGTAATAGGTTTGGAACAAAAAGATTATCACAGTGAACTTTACATCTTTTTACTAGATGAAAACCAACTGAAAACCTTCCAAGAATCACCAGATGAAATGGTAAAAAGAATCCAAGCTCAACAACAGCAAAACGAACTGATAATTGCTAAAAACAAGCAACTAAGAGCTATCAAAAACAAACAAAAGCGAGCTGATTATCTAAAAAAGAAGTATGGTGAAAATTACTTAGATAAAACTAACTTAAAAGATGAAAACAACAATTAA", "aa": "MDIGLISLQYLNIIANYTNKKAIEKITDYLVITSKILAKKADNLLNDHQEESVLEYDLATNNFRDKMIANLVEHKRYCDSLGEFEKLRVNRLAYFSKANEMEQFIKTANDQLVTVEDQLPNYISVLKLFHAMNKLLEMRLSSLLTNKNITIKELSVEQVQKELVLAIKQFNYQTVSLKRVLLKLNHPISLMYFVTAFVALLVLLNNQVIGLEQKDYHSELYIFLLDENQLKTFQESPDEMVKRIQAQQQQNELIIAKNKQLRAIKNKQKRADYLKKKYGENYLDKTNLKDENNN", "aa_hexdigest": "15fb8a2ef922a4dc4c61d5687f1bb74c", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000AF5CFDF", "query_cov": 0.9727891156462585, "subject_cov": 0.8614457831325302, "identity": 0.552, "score": 279.0, "evalue": 6.7e-90, "valid": false, "gene": "scpA", "product": "segregation/condensation protein A", "uniref50_id": "UniRef50_UPI000AF5CFDF", "kegg_orthology_id": "K05896" }, "pscc": { "uniref50_id": "UniRef50_UPI000AF5CFDF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF5CFDF" ], "product": "segregation/condensation protein A" }, "genes": [], "id": "KMADJIBOEG_499", "locus": "GCA000292445_00464" }, { "type": "cds", "sequence": "contig_1", "start": 253500, "stop": 254123, "strand": "+", "frame": 3, "gene": "scpB", "product": "SMC-Scp complex subunit ScpB", "db_xrefs": [ "COG:COG1386", "COG:K", "GO:0005515", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0051301", "GO:0051304", "KEGG:K06024", "RefSeq:WP_014894220.1", "SO:0001217", "UniParc:UPI00027B3BDA", "UniRef:UniRef100_UPI00027B3BDA", "UniRef:UniRef50_P75477", "UniRef:UniRef90_P47456" ], "nt": "ATGAAAACAACAATTAATATTGCCACCCCTACCCTAAAAAAATCTAGCAAAGAAGCTAACTTGATTGCTAGTATCTATGGGTTGTTATTTGTTTGTGGCGCGAAAGGGATCACTTTAAGAGAACTAATTAGGATCTTTAAAAAAGCAGGGATTGAAAAGGTGAAATTAGCACTCTTAGCACTTGAAAGGAAGTTAGCAGATGATGAGCAATCAGGAGTGGAGTTGAAAAAATTTGGTAATAGTTTTTCTTTGGTAACAAAACCAATTATCAAAGACTATCTCCACTTATTATTGGCTCATAAAGTCAAAAATCCCCTTAATTCCAAAGCAATGGAAGTGTTGGCTATCATTGCTTACAACCAACCTTGCACCAGACCCAGAATTAATGAAATTAGGGGAGTTGATTCTTTTCAAATTGTTGATGATCTAATAGCAAAGGAGTTAATTGTGGAGTTAGGGAGAACTGATAAACCAGGTCGACCTTTTATTTATGAAGTGTCAGCTAAGTTCTATGATTTATTTGGCATTGATAGCTTAGATCAACTCCCTAAGATTGAGCATTTTGATCTTGATAAATTTAAGCAAGGTAGCTTTTTTGATTCCAACCGCTATGGTGATGAATAA", "aa": "MKTTINIATPTLKKSSKEANLIASIYGLLFVCGAKGITLRELIRIFKKAGIEKVKLALLALERKLADDEQSGVELKKFGNSFSLVTKPIIKDYLHLLLAHKVKNPLNSKAMEVLAIIAYNQPCTRPRINEIRGVDSFQIVDDLIAKELIVELGRTDKPGRPFIYEVSAKFYDLFGIDSLDQLPKIEHFDLDKFKQGSFFDSNRYGDE", "aa_hexdigest": "1a903e0171f408f57e9b4bf2112ec0d2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3BDA", "ncbi_nrp_id": "WP_014894220.1", "uniref100_id": "UniRef100_UPI00027B3BDA", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3BDA", "RefSeq:WP_014894220.1", "UniRef:UniRef100_UPI00027B3BDA" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3BDA", "uniref90_id": "UniRef90_P47456" }, "psc": { "uniref90_id": "UniRef90_P47456", "gene": "scpB", "product": "SMC-Scp complex subunit ScpB", "uniref50_id": "UniRef50_P75477", "cog_id": "COG1386", "cog_category": "K", "kegg_orthology_id": "K06024", "go_ids": [ "GO:0005515", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0051301", "GO:0051304" ] }, "pscc": { "uniref50_id": "UniRef50_P75477", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75477" ], "product": "Segregation and condensation protein B" }, "genes": [ "scpB" ], "id": "KMADJIBOEG_500", "locus": "GCA000292445_00465" }, { "type": "cds", "sequence": "contig_1", "start": 254142, "stop": 254561, "strand": "+", "frame": 3, "gene": "pfkA", "product": "putative ATP-dependent 6-phosphofructokinase", "db_xrefs": [ "COG:COG0205", "COG:G", "EC:2.7.1.11", "GO:0003872", "GO:0005524", "GO:0005737", "GO:0006002", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P75476", "UniRef:UniRef90_P47457" ], "nt": "ATGGATAAAATAGCTATTTTAACTTCGGGTGGTGATGCTAGTGGGATGAATGCCACCATCGCTTATCTAACCAAATATGCAATTGCAAAGCAATTGGAAGTTTTTTATGTAAAAAACGGTTATTATGGCTTGTATCACAACCATTTTATCACCAGTAAGGAACTTGATTTAACTGACTTTTTCTTTATGGGGGGAACAGTAATAGGATCAAGTCGTTTCAAACAGTTTCAAGATCCTAGCTTACGAAAACAAGCAGTTTTAAACCTCAAAAAACGTGGTATTAACAACCTTGTTGTTATTGGTGGGGATGGGAGTTATATGGGTGCTAAAGCACTCAGTGAATTAGGATTAAACTGCTTTTGTTTACCTGGTACGATCGACAATGATGTCAATTCCAGTGAATTTACCATTGGTTTTTGA", "aa": "MDKIAILTSGGDASGMNATIAYLTKYAIAKQLEVFYVKNGYYGLYHNHFITSKELDLTDFFFMGGTVIGSSRFKQFQDPSLRKQAVLNLKKRGINNLVVIGGDGSYMGAKALSELGLNCFCLPGTIDNDVNSSEFTIGF", "aa_hexdigest": "7d4c7a73cc710480e6da8e0a33dd1a92", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2X1PN14", "query_cov": 0.9928057553956835, "subject_cov": 0.8846153846153846, "identity": 0.514, "score": 135.0, "evalue": 4e-38, "valid": false, "product": "6-phosphofructokinase", "uniref50_id": "UniRef50_A0A2X1PN14" }, "genes": [ "pfkA" ], "truncated": "3-prime", "pseudogene": { "start": 254142, "stop": 254864, "observations": { "mutation": [ 254559 ], "stop": [ 254559 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47457", "query_cov": 1.0, "subject_cov": 0.43034055727554177, "identity": 1.0, "score": 279.0, "evalue": 1.27e-92, "gene_start": 1, "gene_end": 139, "reference_start": 1, "reference_end": 139, "reference_sequence": "MDKIAILTSGGDASGMNATIAYLTKYAIAKQLEVFYVKNGYYGLYHNHFITSKELDLTDFFFMGGTVIGSSRFKQFQDPSLRKQAVLNLKKRGINNLVVIGGDGSYMGAKALSELGLNCFCLPGTIDNDVNSSEFTIGFWTALEAIRVNVEAIYHTTKSHNRLAIIEVMGRDCSDLTIFGGLATNASFVVTSKNSLDLNGFEKAVRKVLQFQNYCVVLVSENIYGKNGLPSLEMVKEHFENNAIKCNLVSLGHTQRGFSPNSIELFQISLMAKHTIDLVVNNANSQVIGMKNNQAVNYDFNTAFNLPKADRTKLLNQVNTAII" }, "paralog": false, "identity": 0.9916666666666667, "score": 473.0, "evalue": 1.47e-171, "description": "Internal stop codon at 254559. Nonsense mutation around 254559.", "psc": { "uniref90_id": "UniRef90_P47457", "gene": "pfkA", "product": "Probable ATP-dependent 6-phosphofructokinase", "ec_ids": [ "2.7.1.11" ], "uniref50_id": "UniRef50_P75476", "cog_id": "COG0205", "cog_category": "G", "go_ids": [ "GO:0005737", "GO:0003872", "GO:0005524", "GO:0046872", "GO:0006002" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47457", "EC:2.7.1.11", "UniRef:UniRef50_P75476", "COG:COG0205", "COG:G", "GO:0005737", "GO:0003872", "GO:0005524", "GO:0046872", "GO:0006002" ] }, "pscc": { "uniref50_id": "UniRef50_P75476", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75476" ], "product": "Probable ATP-dependent 6-phosphofructokinase" } }, "id": "KMADJIBOEG_501", "locus": "GCA000292445_00466" }, { "type": "cds", "sequence": "contig_1", "start": 254646, "stop": 255113, "strand": "+", "frame": 3, "gene": "pfkA", "product": "putative ATP-dependent 6-phosphofructokinase", "db_xrefs": [ "COG:COG0205", "COG:G", "EC:2.7.1.11", "GO:0003872", "GO:0005524", "GO:0005737", "GO:0006002", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P75476", "UniRef:UniRef90_P47457" ], "nt": "ATGGGGCGTGATTGTAGTGATCTGACCATCTTTGGGGGGTTAGCTACTAATGCTAGTTTTGTTGTTACTAGCAAAAATAGCTTGGATCTCAATGGCTTTGAAAAAGCAGTGAGAAAGGTGTTGCAATTCCAGAACTATTGTGTTGTTTTGGTTAGTGAAAACATCTATGGTAAGAACGGTTTACCTAGTTTAAAAATGGTTAAAGAGCACTTTGAAAACAACGCAATTAAGTGTAACCTAGTTTCACTAGGACACACCCAAAGGGGCTTTAGTCCTAATAGTATCGAACTCTTTCAGATTAGTTTAATGGCTAAACACACGATTGATCTGGTTGTAAATAATGCCAACAGTCAAGTAATAGGGATGAAAAACAACCAAGCAGTTAACTATGATTTTAACACTGCTTTTAATTTACCAAAAGCTGATAGAACCAAGTTACTTAACCAAGTTAACACTGCAATTATTTAA", "aa": "MGRDCSDLTIFGGLATNASFVVTSKNSLDLNGFEKAVRKVLQFQNYCVVLVSENIYGKNGLPSLKMVKEHFENNAIKCNLVSLGHTQRGFSPNSIELFQISLMAKHTIDLVVNNANSQVIGMKNNQAVNYDFNTAFNLPKADRTKLLNQVNTAII", "aa_hexdigest": "db21aa2606e45d570afc98f8f63b1588", "start_type": "ATG", "rbs_motif": null, "genes": [ "pfkA" ], "truncated": "5-prime", "pseudogene": { "start": 254346, "stop": 255113, "observations": { "mutation": [ 254559 ], "stop": [ 254559 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47457", "query_cov": 1.0, "subject_cov": 0.47987616099071206, "identity": 0.9940000000000001, "score": 304.0, "evalue": 3.28e-102, "gene_start": 1, "gene_end": 155, "reference_start": 169, "reference_end": 323, "reference_sequence": "MDKIAILTSGGDASGMNATIAYLTKYAIAKQLEVFYVKNGYYGLYHNHFITSKELDLTDFFFMGGTVIGSSRFKQFQDPSLRKQAVLNLKKRGINNLVVIGGDGSYMGAKALSELGLNCFCLPGTIDNDVNSSEFTIGFWTALEAIRVNVEAIYHTTKSHNRLAIIEVMGRDCSDLTIFGGLATNASFVVTSKNSLDLNGFEKAVRKVLQFQNYCVVLVSENIYGKNGLPSLEMVKEHFENNAIKCNLVSLGHTQRGFSPNSIELFQISLMAKHTIDLVVNNANSQVIGMKNNQAVNYDFNTAFNLPKADRTKLLNQVNTAII" }, "paralog": false, "identity": 0.9921568627450981, "score": 500.0, "evalue": 8.66e-182, "description": "Internal stop codon at 254559. Nonsense mutation around 254559.", "psc": { "uniref90_id": "UniRef90_P47457", "gene": "pfkA", "product": "Probable ATP-dependent 6-phosphofructokinase", "ec_ids": [ "2.7.1.11" ], "uniref50_id": "UniRef50_P75476", "cog_id": "COG0205", "cog_category": "G", "go_ids": [ "GO:0005737", "GO:0003872", "GO:0005524", "GO:0046872", "GO:0006002" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47457", "EC:2.7.1.11", "UniRef:UniRef50_P75476", "COG:COG0205", "COG:G", "GO:0005737", "GO:0003872", "GO:0005524", "GO:0046872", "GO:0006002" ] }, "pscc": { "uniref50_id": "UniRef50_P75476", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75476" ], "product": "Probable ATP-dependent 6-phosphofructokinase" } }, "id": "KMADJIBOEG_502", "locus": "GCA000292445_00467" }, { "type": "cds", "sequence": "contig_1", "start": 255116, "stop": 255979, "strand": "+", "frame": 2, "gene": "pyk", "product": "pyruvate kinase", "db_xrefs": [ "COG:COG0469", "COG:G", "EC:2.7.1.40", "GO:0000287", "GO:0004743", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006096", "GO:0016301", "GO:0030955", "KEGG:K00873", "SO:0001217", "UniRef:UniRef50_P78031", "UniRef:UniRef90_P47458" ], "nt": "ATGATTGACCATTTAAAAAGAACAAAGATAATCGCTACCTGTGGCCCAGCTTTAACAAAAAGCTTGGTTAGCTTAAAGATGCTTGATGATAATGAGTATGCAGCTATTAAAAAGGTTGCTTATGCCAACATTGAAGCAATTATTAAAAGTGGGGTTAGTGTGATTAGGCTTAACTTCTCTCATGGTACCCATGAAGAACAACAAGTGAGGATCAAGATAGTAAGGGATGTAGCGAAAGCAATGAACATCCCTGTTTCTATTATGTTAGATACAAATGGTCCTGAGATCAGGATAGTAGAAACTAAAAAAGAGGGTTTGAAAATCACCAAAGATAGTGAAGTGATTATCAACACCATGAGTAAAATGATCGCTAGTGACAACCAGTTTGCTGTCAGTGATGCTAGTGGCAAATACAACATGGTTAATGATGTGAATATAGGTCAGAAAATCCTTGTTGATGATGGTAAGTTAACCCTGGTTGTCACAAGGGTTGACAAACAACATAACCAGGTTATCTGTGTTGCAAAAAACGACCACACAGTTTTCACTAAAAAAAGACTTAACCTACCCAACGCACAGTACTCTATCCCTTTTCTCAGTGAAAAGGATCTGAAGGATATTGACTTTGGTTTAAGCCAAGGTATTGACTATATTGCTGCCTCTTTTGTTAATACTGTTGCAGATATTAAACAACTGAGAGATTATCTGAAATTAAAGAATGCTAGTGGGGTGAAGATCATCGCTAAGATTGAATCTAATCATGCTTTAAATAACATTGATAAGATCATTAAAGCTAGCGATGGGATTATGGTTGCTAGGGGTGATTTGGGCCTTGAAATCCCTTATTACCAAGTCCCTTACTGA", "aa": "MIDHLKRTKIIATCGPALTKSLVSLKMLDDNEYAAIKKVAYANIEAIIKSGVSVIRLNFSHGTHEEQQVRIKIVRDVAKAMNIPVSIMLDTNGPEIRIVETKKEGLKITKDSEVIINTMSKMIASDNQFAVSDASGKYNMVNDVNIGQKILVDDGKLTLVVTRVDKQHNQVICVAKNDHTVFTKKRLNLPNAQYSIPFLSEKDLKDIDFGLSQGIDYIAASFVNTVADIKQLRDYLKLKNASGVKIIAKIESNHALNNIDKIIKASDGIMVARGDLGLEIPYYQVPY", "aa_hexdigest": "792210993a97586d312b7cc544bc540e", "start_type": "ATG", "rbs_motif": null, "genes": [ "pyk" ], "truncated": "3-prime", "pseudogene": { "start": 255116, "stop": 256282, "observations": { "mutation": [ 255977 ], "stop": [ 255977 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47458", "query_cov": 1.0, "subject_cov": 0.5649606299212598, "identity": 1.0, "score": 556.0, "evalue": 1.49e-196, "gene_start": 1, "gene_end": 287, "reference_start": 1, "reference_end": 287, "reference_sequence": "MIDHLKRTKIIATCGPALTKSLVSLKMLDDNEYAAIKKVAYANIEAIIKSGVSVIRLNFSHGTHEEQQVRIKIVRDVAKAMNIPVSIMLDTNGPEIRIVETKKEGLKITKDSEVIINTMSKMIASDNQFAVSDASGKYNMVNDVNIGQKILVDDGKLTLVVTRVDKQHNQVICVAKNDHTVFTKKRLNLPNAQYSIPFLSEKDLKDIDFGLSQGIDYIAASFVNTVADIKQLRDYLKLKNASGVKIIAKIESNHALNNIDKIIKASDGIMVARGDLGLEIPYYQVPYWQRYMIKACRFFNKRSITATQMLDSLEKNIQPTRAEVTDVYFAVDRGNDATMLSGETASGLYPLNAVAVMQKIDKQSETFFDYQYNVNYYLKNSTANKSRFWHNVVLPLTKKTVPKRKLVNSAFKYDFIVYPTNNINRIYALSNARLAAAVIILTNNKRVYTGHGVDYGIFCYLIDKNPNQLTKAELIELAWKAINHYQAYGDLEKLKQCLAVYNETIINL" }, "paralog": false, "identity": 0.9974226804123711, "score": 752.0, "evalue": 2.73e-276, "description": "Internal stop codon at 255977. Nonsense mutation around 255977.", "psc": { "uniref90_id": "UniRef90_P47458", "gene": "pyk", "product": "pyruvate kinase", "ec_ids": [ "2.7.1.40" ], "uniref50_id": "UniRef50_P78031", "cog_id": "COG0469", "cog_category": "G", "kegg_orthology_id": "K00873", "go_ids": [ "GO:0000287", "GO:0004743", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006096", "GO:0016301", "GO:0030955" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47458", "EC:2.7.1.40", "UniRef:UniRef50_P78031", "COG:COG0469", "COG:G", "KEGG:K00873", "GO:0000287", "GO:0004743", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006096", "GO:0016301", "GO:0030955" ] }, "pscc": { "uniref50_id": "UniRef50_P78031", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78031" ], "product": "Pyruvate kinase" } }, "id": "KMADJIBOEG_503", "locus": "GCA000292445_00468" }, { "type": "cds", "sequence": "contig_1", "start": 255989, "stop": 256282, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00224.27" ], "nt": "ATGATTAAAGCTTGTCGCTTTTTTAACAAGCGTTCTATTACTGCAACCCAAATGCTTGATTCACTAGAAAAAAACATCCAACCAACCCGAGCTGAAGTGACTGATGTTTACTTTGCAGTTGATCGGGGTAATGATGCAACTATGTTAAGTGGGGAAACTGCTAGTGGGCTTTACCCTTTAAATGCAGTAGCGGTGATGCAAAAGATTGATAAACAATCAGAAACCTTCTTTGATTACCAGTATAACGTTAACTATTATTTGAAAAACTCCACGGCAAATAAAAGTAGGTTTTGA", "aa": "MIKACRFFNKRSITATQMLDSLEKNIQPTRAEVTDVYFAVDRGNDATMLSGETASGLYPLNAVAVMQKIDKQSETFFDYQYNVNYYLKNSTANKSRF", "aa_hexdigest": "d15edec308c290aec65533dd551eabf1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00224.27", "name": "PK", "length": 63, "aa_cov": 0.6494845360824743, "hmm_cov": 1.0, "evalue": 2.2893668034254806e-23, "score": 77.8996353149414, "start": 1, "stop": 63 } ], "seq_stats": { "molecular_weight": 11096.425699999994, "isoelectric_point": 8.732000923156736 }, "id": "KMADJIBOEG_504", "locus": "GCA000292445_00469" }, { "type": "tRNA", "sequence": "contig_1", "start": 256679, "stop": 256755, "strand": "+", "gene": "trnC", "product": "tRNA-Cys(gca)", "amino_acid": "Cys", "anti_codon": "gca", "score": 60.0, "nt": "GGTGCCTTAGCCAAGTGGACTCAAGGCCTGGAGCTGCAACCTCCATATCGTCAGTTCGAATCTGACAGGCACCTCCA", "db_xrefs": [ "SO:0000258" ], "anti_codon_pos": [ 256714, 256716 ], "id": "KMADJIBOEG_1", "locus": "GCA000292445_00470" }, { "type": "tRNA", "sequence": "contig_1", "start": 256790, "stop": 256866, "strand": "+", "gene": "trnP", "product": "tRNA-Pro(tgg)", "amino_acid": "Pro", "anti_codon": "tgg", "score": 83.1, "nt": "CGGGAAGTAGCTTAGTTTGGTAGAGCACTTGGTTTGGGACCAAGGGGTCGCAGGTTCAAATCCTGTCTTCCCGACCA", "db_xrefs": [ "SO:0000268" ], "anti_codon_pos": [ 256807, 256809 ], "id": "KMADJIBOEG_2", "locus": "GCA000292445_00471" }, { "type": "tRNA", "sequence": "contig_1", "start": 256870, "stop": 256946, "strand": "+", "gene": "trnM", "product": "tRNA-Met(cat)", "amino_acid": "Met", "anti_codon": "cat", "score": 81.0, "nt": "GGCTGGATACCTCAGTTGGTTAGAGGGCCCGGTTCATACCCGGGTTGTCGTGAGTTCGAGTCTCACTCCAGCCACCA", "db_xrefs": [ "SO:0000266" ], "anti_codon_pos": [ 256904, 256906 ], "id": "KMADJIBOEG_3", "locus": "GCA000292445_00472" }, { "type": "tRNA", "sequence": "contig_1", "start": 256966, "stop": 257042, "strand": "+", "gene": "trnI", "product": "tRNA-Ile2(cat)", "amino_acid": "Ile2", "anti_codon": "cat", "score": 74.3, "nt": "GGATCTATAGCTCAATTGGTTAGAGCCCCCGACTCATAATCGGTCTGTTACAGGTTCAAGTCCTGTTAGATCCACCA", "db_xrefs": [ "SO:0000263" ], "id": "KMADJIBOEG_4", "locus": "GCA000292445_00473" }, { "type": "tRNA", "sequence": "contig_1", "start": 257080, "stop": 257171, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(tga)", "amino_acid": "Ser", "anti_codon": "tga", "score": 75.0, "nt": "GGAGACTTACCCAAGCGGCTGAAGGGTTCGGTCTTGAAAACCGAGAGGTGCTTTATAAGCACGCGAGGGTTCAAATCCCTCAGTCTCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 257099, 257101 ], "id": "KMADJIBOEG_5", "locus": "GCA000292445_00474" }, { "type": "tRNA", "sequence": "contig_1", "start": 257185, "stop": 257261, "strand": "+", "gene": "trnfM", "product": "tRNA-fMet(cat)", "amino_acid": "fMet", "anti_codon": "cat", "score": 78.9, "nt": "CGCGGGATAGAGCAGTTGGTCAGCTCGTCAGGCTCATAATCTGAAGGTCGAGGGTTCAAATCCCTCTCCCGCAACCA", "db_xrefs": [ "SO:0000266" ], "id": "KMADJIBOEG_6", "locus": "GCA000292445_00475" }, { "type": "tRNA", "sequence": "contig_1", "start": 257263, "stop": 257336, "strand": "+", "gene": "trnD", "product": "tRNA-Asp(gtc)", "amino_acid": "Asp", "anti_codon": "gtc", "score": 55.6, "nt": "GGTTCCATGGTGTAGTGATAACATATCTCCCTGTCACGGAGGGGTTGCGGGTTTGATTCCCGTTGGAACCGCCA", "db_xrefs": [ "SO:0000256" ], "anti_codon_pos": [ 257295, 257297 ], "id": "KMADJIBOEG_7", "locus": "GCA000292445_00476" }, { "type": "tRNA", "sequence": "contig_1", "start": 257339, "stop": 257414, "strand": "+", "gene": "trnF", "product": "tRNA-Phe(gaa)", "amino_acid": "Phe", "anti_codon": "gaa", "score": 77.8, "nt": "GGTCTTGTAGCTCAGTCGGTAGAGCAACGGTCTGAAGAACCGTGTGTCGGCAGTTCGATTCTGCCCGAGACCACCA", "db_xrefs": [ "SO:0000267" ], "anti_codon_pos": [ 257372, 257374 ], "id": "KMADJIBOEG_8", "locus": "GCA000292445_00477" }, { "type": "cds", "sequence": "contig_1", "start": 257562, "stop": 258683, "strand": "+", "frame": 3, "gene": null, "product": "cytadherence-associated protein P65", "db_xrefs": [ "BlastRules:WP_010869376", "BlastRules:WP_010874665", "RefSeq:WP_014894223.1", "SO:0001217", "UniParc:UPI00027B3BDD", "UniRef:UniRef100_UPI00027B3BDD", "UniRef:UniRef50_P47459", "UniRef:UniRef90_P47459" ], "nt": "ATGGAAAAAAATAGATCAGCTTTTCAACAAAACCAACAAGCATCAAACCAACCTTTTAACCAAGATCAAAACCAGTATTACCAAGATCCTAACCAACAACAATTTAACCAACCTGGTTTTGATCCAAATCAACAACAATTTAATCAACCAGGATTTGACCCTAACCAGCAATATTATCAAGATCCCAATCAACAACAATTTAATCAAGCTGGTTTTGATCAAAACCAACAGTATTACCAAGATCCCAATCAACAACAATTTAATCAATCAGGTTTTGATCCAAACCAACAATATTATCAAGATCCCAATCAACAACAATTTAATCAAGCTGGTTTTGATCAAAACCAACAGTATTACCAAGATCCCAATCAACAACAATTTAATCAATCAGGTTTTGATCAAAACCAGTACTATCAAGATCCTAATCAGCAACAATTTAACCAGCCTAGTTTTGATCTAAATAACCAACAATTTAACCAACCTGGATTCAACCAATCCCCAGCATTTGAAATCACACCTCAAGAGCAAAAAGCTGAACAGGAAATGTTTGGTGAAGAACCACCTCAAGTAGTTAGAGAGATCCATGAACTACCATTTGAAAAGATCCGTTCTTTTTTACAAAGTGATTTTGATAGCTATAACTTTCGCTTAAACTCACTTAAAAGCAAACTGGATAATGCCTTGTATTCACTTGATAAAACAATCCAAAACACCAATGAAAACACTGCTAATCTAGAAGCAATTAGACATAACTTAGAACAAAAGATTCAAAACCAAAGCAAGCAATTAAGAACTAACTTTGATACCCAAAAGCTTGATGATAAGATCAATGAATTGGAGATCAGAATGCAAAAACTAACCAGGAATTTTGAATCTCTAAGTGAACTTTCAAAGCACAACTCTTATCCTAATTACTATGAAAAATTGTTACCAAATGGTGGTGATAGTATGACCAATGTCTTTGAAAAAGCACTAATGATGAATTTATTGAGAACTACATTACCCCCTCAACCCCAAGTTCAATACTACCCTCAACCCTATCCATACATAAGACCTTACTATGATGAACCTATTTACGCTGGGTTTAGAAGAAGGGGTTACCGTGATGACTTCTATGAATAA", "aa": "MEKNRSAFQQNQQASNQPFNQDQNQYYQDPNQQQFNQPGFDPNQQQFNQPGFDPNQQYYQDPNQQQFNQAGFDQNQQYYQDPNQQQFNQSGFDPNQQYYQDPNQQQFNQAGFDQNQQYYQDPNQQQFNQSGFDQNQYYQDPNQQQFNQPSFDLNNQQFNQPGFNQSPAFEITPQEQKAEQEMFGEEPPQVVREIHELPFEKIRSFLQSDFDSYNFRLNSLKSKLDNALYSLDKTIQNTNENTANLEAIRHNLEQKIQNQSKQLRTNFDTQKLDDKINELEIRMQKLTRNFESLSELSKHNSYPNYYEKLLPNGGDSMTNVFEKALMMNLLRTTLPPQPQVQYYPQPYPYIRPYYDEPIYAGFRRRGYRDDFYE", "aa_hexdigest": "b974aab3efba8bff6a9fd5e221f02c74", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3BDD", "ncbi_nrp_id": "WP_014894223.1", "uniref100_id": "UniRef100_UPI00027B3BDD", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3BDD", "RefSeq:WP_014894223.1", "UniRef:UniRef100_UPI00027B3BDD" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3BDD", "uniref90_id": "UniRef90_P47459" }, "psc": { "uniref90_id": "UniRef90_P47459", "product": "Proline-rich P65 protein homolog", "uniref50_id": "UniRef50_P47459" }, "pscc": { "uniref50_id": "UniRef50_P47459", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47459" ], "product": "Proline-rich P65 protein homolog" }, "expert": [ { "type": "expert_proteins", "source": "BlastRules", "rank": 70, "id": "WP_010869376", "gene": null, "product": "cytadherence-associated protein P65", "query_cov": 1.0, "subject_cov": 1.0026881720430108, "identity": 0.968, "score": 426.0, "evalue": 2.88e-149, "db_xrefs": [ "BlastRules:WP_010869376", "BlastRules:WP_010874665" ] } ], "genes": [], "id": "KMADJIBOEG_505", "locus": "GCA000292445_00478" }, { "type": "cds", "sequence": "contig_1", "start": 258622, "stop": 258714, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGAACCTATTTACGCTGGGTTTAGAAGAAGGGGTTACCGTGATGACTTCTATGAATAAAAAGCGTGTTTTAACTAATGAAACCATTTGA", "aa": "MMNLFTLGLEEGVTVMTSMNKKRVLTNETI", "aa_hexdigest": "c7d305b1308506acc292e516438af4c2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3402.0336000000007, "isoelectric_point": 5.986078071594237 }, "id": "KMADJIBOEG_506", "locus": "GCA000292445_00479" }, { "type": "cds", "sequence": "contig_1", "start": 258701, "stop": 261853, "strand": "+", "frame": 2, "gene": "hmw2", "product": "Cytadherence high molecular weight protein 2", "db_xrefs": [ "COG:COG1196", "COG:D", "GO:0020035", "SO:0001217", "UniRef:UniRef50_P75471", "UniRef:UniRef90_P47460" ], "nt": "ATGAAACCATTTGATAAAAAACCTTCGCTGCAACCAATTTATGACATTGGTTTTGATGATGGTTATCTCCAAAGTGAGTATGAAAAAAATCGTTCTAAAACCGATGTTGATAAGATCGAAAACCAGCTTTTAAAAGAGATTAAAAGCCTGGAAGATGAACTTAAAAACCTTAAGGGCTTGAAGAATCAAGCAGAAGATAATCCTGAACTTGATAAAAAGATTAACCACTTGGAAGTTGATCTAAACCGTTTGGTTAATGAATATAAAAACTTCCAGTTCCAAAAGAACCACATGGTTGATAAGGTTAGTGAACTTGATAACTTAACCCGTTTTTATAAGAATGAACTAACCCGCTTACAACAAGAAAACGCTGATTTTCTCAACTCCAAGTATGCTAATTTAGCTAACTTCCAAGCTAACTACCACAATAAACTAAATGATTTTCACCGCTTAATAGAAAATCAAAACCAAACCATTAACCGCTTAAACCAAAAGATTAATGGTAACCAAAATCTGATTGATAATAACGTTGCTTTACTGCAAAACCCCAACATCACAGTTGAAAAAAAGAACTACTTACTAAATGTTATTGATCAACTTTACAATGAGCTTGATCAACTTGAGAATCAAAAAAGATTATTAAGTATTGAGTATGAAAATACCTATAGAGAGTTAGTTAGTGCAGATAATGAACTGCAAAATGTTTATGAAAACATCGATCAAAATCAGATCCAGTTTAAACACCAATACCAAACTTATAGAGATGAGTTAAGTCAACTTGAGCGCAAGATCCAGCTCACCAAACAAGAGTTAGTTGATAAAGAATCAGCACTAAGAGTAAAGATAGATGATGCTGATTTTTACATTAATGCCCGTTTAGCTGAACTTGATGATGTAGCTAAACAACTTAGCTTTCAAGATGGTATTACCAAGCAAAATGCTCAACATGTTGAGGATAAGTTAGTTGCTTTAAACAAAGAAAAAGACCGTTTAAATACCCAAAAAGAGGCCTTTTTTAACTTAAGACAATCTGCTTTAATTGATATCAATAAACTCCAGCAGGAAAATGAACTGTTTGCTAAGCACTTAGAACACCAGCAAAATGAGTTTGAACAAAAACAGTCTGATAGCCTCTTAAAGCTTGAAACTGAATATAAGGCTTTACAACACAAGATTAATGAATTTAAAAATGAAAGTGCCACTAAGAGCGAGGAACTTTTAAACCAAGAACGGGAACTATTTGAAAAACGCAGGGAAATTGACACGCTTTTAACCCAAGCATCTTTGGAATATGAACACCAACGTGAGTCAAGTCAACTACTCAAAGATAAGCAGAATGAAGTAAAACAACACTTCCAAAACTTAGAGTATGCTAAAAAGGAGCTAGACAAAGAAAGAAACCTCTTAGATCAACAAAAAAAAGTTGATAGTGAAGCAATCTTTCAACTCAAAGAAAAGGTTGCTCAAGAACGTAAGGAGCTTGAAGAACTATACCTTGTTAAAAAACAAAAACAAGATCAGAAGGAAAATGAACTGTTGTTTTTTGAAAAGCAGTTAAAACAACACCAAGCAGATTTTGAAAATGAATTGGAAGCTAAACAACAGGAGTTGTTTGAAGCTAAACATGCATTGGAACGTTCCTTTATCAAACTTGAAGATAAAGAAAAAGATCTTAACACTAAAGCACAACAGATTGCCAATGAGTTTTCCCAACTAAAAACTGATAAGTCAAAGAGTGCTGATTTTGAACTAATGTTGCAAAATGAGTATGAAAACTTGCAACAAGAAAAACAAAAGTTATTCCAAGAACGTACTTACTTTGAAAGGAATGCTGCGGTTTTATCAAACCGGTTACAACAAAAACGTGAGGAGTTATTACAACAAAAAGAAACGCTTGATCAGCTTACAAAAAGCTTTGAGCAAGAACGGTTAATCAACCAAAGGGAACACAAGGAGTTGGTTGCATCAGTTGAAAAACAAAAGGAGATATTGGGCAAAAAACTCCAAGATTTTTCCCAAACTTCACTGAACGCTTCTAAAAATTTAGCTGAACGGGAGATGGCAATCAAGTTTAAAGAAAAGGAGATAGAAGCAACTGAAAAGCAACTGTTAAATGATGTTAATAATGCTGAAGTTATCCAAGCAGACTTAGCACAACTCAACCAATCACTTAACCAAGAACGCAGTGAATTGCAAAACGCCAAACAAAGGATTGCTGATTTTCACAATGATTCACTAAAAAAACTCAATGAGTATGAACTTAGCTTACAAAAACGGTTGCAAGAATTACAAACCCTTGAGGCTAACCAAAAACAACATTCGTATCAAAATCAAGCTTACTTTGAAGGTGAACTTGATAAACTTAACAGAGAAAAACAAGCTTTTTTGAACTTACGTAAGAAACAAACTATGGAGGTTGATGCTATTAAACAAAGGTTGAGTGATAAACATCAAGCTTTAAATATGCAACAAGCAGAGCTAGATAGAAAAACCCATGAGTTAAATAATGCTTTTTTAAACCATGATGCGGATCAAAAGAGTCTACAGGACCAACTAGCAACTGTTAAAGAGACCCAAAAACTAATTGATTTAGAACGTAGTGCACTGCTTGAAAAGCAACGTGAGTTTGCTGAAAATGTTGCTGGTTTTAAGCGCCATTGGTCTAATAAAACTAGTCAACTCCAAAAGGTTTATGAACTGACCAAAAAACAGGAAAGTGAGCAAACCCAAAAGGAAACAGAACTAAAGATTGCTTTTAGTGATCTACAAAAAGACTATCAGGTTTTTGAACTCCAAAAGGACCAAGAATTTAGACAAATTGAAGCTAAGCAACGTGAACTTGACAAGTTAGCTGAAAAAAATAATCAGGTCAAACTAGAACTTGATAACAGGTTTCAAGCGCTGCAAAACCAAAAGCAAGACACAGTACAAGCTCAGCTAGAACTGGAACGTGAACAACACCAGTTAAACCTTGAGCAAACTGCTTTCAACCAAGCTAATGAATCACTTTTAAAACAACGTGAACAACTCACCAAAAAGATCCAAGCTTTCCACTATGAGTTGAAAAAGCGTAACCAATTCTTAGCTTTAAAAGGGAAAAGGTTGTTTGCAAAAGAACAAGATCAACAACGCAAAGATCAGGAGATCAACTGA", "aa": "MKPFDKKPSLQPIYDIGFDDGYLQSEYEKNRSKTDVDKIENQLLKEIKSLEDELKNLKGLKNQAEDNPELDKKINHLEVDLNRLVNEYKNFQFQKNHMVDKVSELDNLTRFYKNELTRLQQENADFLNSKYANLANFQANYHNKLNDFHRLIENQNQTINRLNQKINGNQNLIDNNVALLQNPNITVEKKNYLLNVIDQLYNELDQLENQKRLLSIEYENTYRELVSADNELQNVYENIDQNQIQFKHQYQTYRDELSQLERKIQLTKQELVDKESALRVKIDDADFYINARLAELDDVAKQLSFQDGITKQNAQHVEDKLVALNKEKDRLNTQKEAFFNLRQSALIDINKLQQENELFAKHLEHQQNEFEQKQSDSLLKLETEYKALQHKINEFKNESATKSEELLNQERELFEKRREIDTLLTQASLEYEHQRESSQLLKDKQNEVKQHFQNLEYAKKELDKERNLLDQQKKVDSEAIFQLKEKVAQERKELEELYLVKKQKQDQKENELLFFEKQLKQHQADFENELEAKQQELFEAKHALERSFIKLEDKEKDLNTKAQQIANEFSQLKTDKSKSADFELMLQNEYENLQQEKQKLFQERTYFERNAAVLSNRLQQKREELLQQKETLDQLTKSFEQERLINQREHKELVASVEKQKEILGKKLQDFSQTSLNASKNLAEREMAIKFKEKEIEATEKQLLNDVNNAEVIQADLAQLNQSLNQERSELQNAKQRIADFHNDSLKKLNEYELSLQKRLQELQTLEANQKQHSYQNQAYFEGELDKLNREKQAFLNLRKKQTMEVDAIKQRLSDKHQALNMQQAELDRKTHELNNAFLNHDADQKSLQDQLATVKETQKLIDLERSALLEKQREFAENVAGFKRHWSNKTSQLQKVYELTKKQESEQTQKETELKIAFSDLQKDYQVFELQKDQEFRQIEAKQRELDKLAEKNNQVKLELDNRFQALQNQKQDTVQAQLELEREQHQLNLEQTAFNQANESLLKQREQLTKKIQAFHYELKKRNQFLALKGKRLFAKEQDQQRKDQEIN", "aa_hexdigest": "52db5d2e56a5c4fd6453495e5223b4d2", "start_type": "ATG", "rbs_motif": null, "genes": [ "hmw2" ], "truncated": "3-prime", "pseudogene": { "start": 258701, "stop": 262156, "observations": { "mutation": [ 261851 ], "stop": [ 261851 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47460", "query_cov": 1.0, "subject_cov": 0.5817174515235457, "identity": 0.9990000000000001, "score": 1575.0, "evalue": 0.0, "gene_start": 1, "gene_end": 1050, "reference_start": 1, "reference_end": 1050, "reference_sequence": "MKPFDKKPSLQPIYDIGFDDGYLQSEYEKNRSKTDVDKIENQLLKEIKSLEDELKNLKGLKNQAEDNPELDKKINHLEVDLNRLVNEYKNFQFQKNHMVDKVSELDNLTRFYKNELTRLQQENADFLNSKYANLANFQANYHNKLNDFHRLIENQNQTINRLNQKINGNQNLIDNNVALLQNPNITVEKKNYLLNVIDQLYNELDQLENQKRLLSIEYENTYRELVSADNELQNVYENIDQNQIQFKHQYQTYRDELSQLERKIQLTKQELVDKESALRVKIDDADFYINARLAELDDVAKQLSFQDGITKQNAQHVEDKLVALNKEKDRLNTQKEAFFNLRQSALIDINKLQQENELFAKHLEHQQNEFEQKQSDSLLKLETEYKALQHKINEFKNESATKSEELLNQERELFEKRREIDTLLTQASLEYEHQRESSQLLKDKQNEVKQHFQNLEYAKKELDKERNLLDQQKKVDSEAIFQLKEKVAQERKELEELYLVKKQKQDQKENELLFFEKQLKQHQADFENELEAKQQELFEAKHALERSFIKLEDKEKDLNTKAQQIANEFSQLKTDKSKSADFELMLQNEYENLQQEKQKLFQERTYFERNAAVLSNRLQQKREELLQQKETLDQLTKSFEQERLINQREHKELVASVEKQKEILGKKLQDFSQTSLNASKNLAEREMAIKFKEKEIEATEKQLLNDVNNAEVIQADLAQLNQSLNQERSELQNAKQRIADFHNDSLKKLNEYELSLQKRLQELQTLEANQKQHSYQNQAYFEGELDKLNREKQAFLNLRKKQTMEVDAIKQRLSDKHQALNMQQAELDRKTHELNNAFLNHDADQKSLQDQLATVKETQKLIDLERSALLEKQREFAENVAGFKRHWSNKTSQLQKIYELTKKQESEQTQKETELKIAFSDLQKDYQVFELQKDQEFRQIEAKQRELDKLAEKNNQVKLELDNRFQALQNQKQDTVQAQLELEREQHQLNLEQTAFNQANESLLKQREQLTKKIQAFHYELKKRNQFLALKGKRLFAKEQDQQRKDQEINWRFKQFEKEYTDFDEAKKRELEELEKIRRSLSQSNVELERKREKLATDFTNLNKVQHNTQINRDQLNSQIRQFLLERKNFQRFSNEANAKKAFLIKRLRSFASNLKLQKEALAIQKLEFDKRDEQQKKELQQATLQLEQFKFEKQNFDIEKQRQLVAIKTQCEKLSDEKKALNQKLVELKNLSQTYLANKNKAEYSQQQLQQKYTNLLDLKENLERTKDQLDKKHRSIFARLTKFANDLRFEKKQLLKAQRIVDDKNRLLKENERNLHFLSNETERKRAVLEDQISYFEKQRKQATDAILASHKEVKKKEGELQKLLVELETRKTKLNNDFAKFSRQREEFENQRLKLLELQKTLQTQTNSNNFKTKAIQEIENSYKRGMEELNFQKKEFDKNKSRLYEYFRKMRDEIERKESQVKLVLKETQRKANLLEAQANKLNIEKNTIDFKEKELKAFKDKVDQDIDSTNKQRKELNELLNENKLLQQSLIERERAINSKDSLLNKKIETIKRQLHDKEMRVLRLVDRMKLAEQKYQTEINRLRTQTFDSEKQDIKNFFPPLFKINGNDMAFPYLYPWLYPQQKQDDNTLQIRQLFEQQLQFMQQRYENELNELRRQRNLLEKKLDQIQLESQLNNKQSEFSKVESMMEKLLEKTESRLNDFDQKINYLTKKVNQHNTYQPSSYQPTPSYQDSDKQQLLFRIQELEKQNLFQQQFQPAPAVVQQPTSFAAPNITKQQQIAQLNAEINNIKRLIAQKAASK" }, "paralog": false, "identity": 0.998262380538662, "score": 1906.0, "evalue": 0.0, "description": "Internal stop codon at 261851. Nonsense mutation around 261851.", "psc": { "uniref90_id": "UniRef90_P47460", "gene": "hmw2", "product": "Cytadherence high molecular weight protein 2", "uniref50_id": "UniRef50_P75471", "cog_id": "COG1196", "cog_category": "D", "go_ids": [ "GO:0020035" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47460", "UniRef:UniRef50_P75471", "COG:COG1196", "COG:D", "GO:0020035" ] }, "pscc": { "uniref50_id": "UniRef50_P75471", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75471" ], "product": "Cytadherence high molecular weight protein 2" } }, "id": "KMADJIBOEG_507", "locus": "GCA000292445_00480" }, { "type": "cds", "sequence": "contig_1", "start": 262628, "stop": 263569, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGAAAATGAACGTAACCTCCATTTCCTTTCCAATGAAACAGAACGAAAACGAGCAGTTCTCGAAGATCAAATTTCTTACTTTGAAAAACAACGTAAACAAGCTACTGATGCGATCCTAGCATCACATAAAGAAGTTAAAAAGAAGGAAGGTGAACTGCAAAAGTTACTGGTTGAATTAGAAACAAGAAAAACCAAACTCAACAATGATTTTGCAAAATTCTCAAGACAACGTGAAGAGTTTGAAAACCAACGCTTAAAGCTCTTGGAACTGCAAAAAACCCTGCAAACCCAAACTAATTCCAACAACTTTAAAACCAAAGCAATCCAAGAGATTGAAAACAGTTATAAAAGGGGGATGGAAGAACTTAACTTCCAAAAGAAGGAGTTTGATAAGAATAAATCACGCTTATATGAATACTTTAGAAAGATGCGTGATGAGATTGAAAGAAAGGAAAGTCAGGTTAAGTTAGTTTTAAAAGAGACCCAAAGGAAAGCCAACCTCTTAGAAGCACAAGCCAACAAACTTAACATTGAAAAAAACACTATTGACTTTAAAGAAAAAGAGTTAAAAGCCTTTAAAGATAAGGTTGATCAAGACATTGATTCAACCAATAAACAACGCAAGGAGTTAAATGAGCTTTTAAATGAAAACAAGTTATTACAACAATCACTAATCGAAAGAGAAAGGGCTATTAATTCCAAAGATTCACTTCTAAATAAGAAGATAGAAACGATTAAACGCCAACTCCATGATAAGGAGATGCGGGTGTTGCGCTTGGTTGATAGAATGAAATTAGCAGAACAGAAATACCAAACAGAAATCAACCGCTTAAGAACCCAAACTTTCGATTCTGAAAAACAAGACATTAAAAACTTCTTCCCACCGTTATTTAAAATTAACGGTAATGATATGGCCTTTCCTTACTTATACCCCTGA", "aa": "MKENERNLHFLSNETERKRAVLEDQISYFEKQRKQATDAILASHKEVKKKEGELQKLLVELETRKTKLNNDFAKFSRQREEFENQRLKLLELQKTLQTQTNSNNFKTKAIQEIENSYKRGMEELNFQKKEFDKNKSRLYEYFRKMRDEIERKESQVKLVLKETQRKANLLEAQANKLNIEKNTIDFKEKELKAFKDKVDQDIDSTNKQRKELNELLNENKLLQQSLIERERAINSKDSLLNKKIETIKRQLHDKEMRVLRLVDRMKLAEQKYQTEINRLRTQTFDSEKQDIKNFFPPLFKINGNDMAFPYLYP", "aa_hexdigest": "b6ff1330878229225a030002b5dc376f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 37801.716900000036, "isoelectric_point": 9.508780479431152 }, "id": "KMADJIBOEG_508", "locus": "GCA000292445_00481" }, { "type": "cds", "sequence": "contig_1", "start": 263642, "stop": 264118, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACAACGCTATGAAAATGAGTTAAATGAATTGCGTAGACAACGTAATTTACTTGAGAAAAAACTTGATCAAATCCAACTAGAATCCCAGCTTAACAATAAGCAAAGTGAGTTTAGTAAGGTGGAATCAATGATGGAAAAACTACTTGAAAAAACTGAGAGTAGGTTAAATGATTTTGATCAGAAAATTAACTATCTCACCAAGAAAGTTAACCAACACAACACCTATCAACCAAGTTCCTATCAACCAACTCCTTCTTATCAAGACAGTGATAAACAACAGTTGTTATTTAGAATCCAAGAACTGGAAAAACAAAACTTATTCCAACAACAATTTCAACCTGCACCAGCTGTTGTCCAACAACCTACTAGTTTTGCAGCCCCTAACATCACCAAACAACAGCAAATTGCCCAACTTAATGCTGAAATTAACAACATTAAAAGGTTGATTGCCCAAAAAGCAGCAAGTAAATAA", "aa": "MQQRYENELNELRRQRNLLEKKLDQIQLESQLNNKQSEFSKVESMMEKLLEKTESRLNDFDQKINYLTKKVNQHNTYQPSSYQPTPSYQDSDKQQLLFRIQELEKQNLFQQQFQPAPAVVQQPTSFAAPNITKQQQIAQLNAEINNIKRLIAQKAASK", "aa_hexdigest": "c866201a75b0dae332d05662976d194c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18683.82479999999, "isoelectric_point": 9.245685005187987 }, "id": "KMADJIBOEG_509", "locus": "GCA000292445_00482" }, { "type": "cds", "sequence": "contig_1", "start": 264121, "stop": 264816, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MG218-1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZB78", "UniRef:UniRef90_Q9ZB78" ], "nt": "ATGGTTAATAATGAATATCAACAACTAAACACTTTAGTTGAGAGTGATGATGAAGCGGATCTTGTGATTGCTAACCTAGTTAAACAACTCAATGAACTAAAGCAAATCCTTGTTTCACTAGATAATCAAGAAGCAAGTGCCACTGCAGTTACTGATAAAAAGGAAGAGGAATACAACCAAAACCAATCCAGTTTCCATAACTTCAGCAAAGAAACACTGCAAAAGCAAGCAAAACGTGGTTTTCTTTTACTGGAACGCTGTTCGTTGGTTGGGTTACAACAACTAGAGTTGGAGTATGTTAATTTGTTGGGCAGAAGTTTTGATTCTTATCAACAAAAAACAGAGCTTTTAAACAACTTAAAGGAGCTTGTTGATGAACATTTCAGTGATACTGAAAAAATTATCAATACCCTTGAAAAGATCTTTGATGTTATTGGCGGTAGTGAATATACCCCTGTCTTAAACTCGTTTTTTAACAAGCTTTTAAGTGATCCTGATCCAATCCAACGGGAAATTGGCTTAAGACAATTTATCATCACTCTCCGTCAGCGCTTTAAAAAGTTATCACAAAAGATTGACAGTTCTCTCAAACAGATAGAAACAGAGGCTAAAATAGCCACTGAACAGGTTCAAAATAGTGAAGTGATGTTCGGTCCCCCTGATATTGCTAATGATCATGAGTTAAACCTGAACTGA", "aa": "MVNNEYQQLNTLVESDDEADLVIANLVKQLNELKQILVSLDNQEASATAVTDKKEEEYNQNQSSFHNFSKETLQKQAKRGFLLLERCSLVGLQQLELEYVNLLGRSFDSYQQKTELLNNLKELVDEHFSDTEKIINTLEKIFDVIGGSEYTPVLNSFFNKLLSDPDPIQREIGLRQFIITLRQRFKKLSQKIDSSLKQIETEAKIATEQVQNSEVMFGPPDIANDHELNLN", "aa_hexdigest": "13caf5b023013f741b427089ab755c8f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 264121, "stop": 265119, "observations": { "mutation": [ 264814 ], "stop": [ 264814 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9ZB78", "query_cov": 1.0, "subject_cov": 0.6676300578034682, "identity": 1.0, "score": 431.0, "evalue": 2.07e-150, "gene_start": 1, "gene_end": 231, "reference_start": 1, "reference_end": 231, "reference_sequence": "MVNNEYQQLNTLVESDDEADLVIANLVKQLNELKQILVSLDNQEASATAVTDKKEEEYNQNQSSFHNFSKETLQKQAKRGFLLLERCSLVGLQQLELEYVNLLGRSFDSYQQKTELLNNLKELVDEHFSDTEKIINTLEKIFDVIGGSEYTPVLNSFFNKLLSDPDPIQREIGLRQFIITLRQRFKKLSQKIDSSLKQIETEAKIATEQVQNSEVMFGPPDIANDHELNLNWPDSETDAILSSMENELEAALLAKHQEEPPLIVTPPSLIKPTVSQPEVEVVTPTNNTNFQPQVDLKPTDLKKQQKKKPLNFITRPVFKSNLPPKLSKDDIVHYAHQLLEKNTHNE" }, "paralog": false, "identity": 0.9969879518072289, "score": 644.0, "evalue": 4.47e-237, "description": "Internal stop codon at 264814. Nonsense mutation around 264814.", "psc": { "uniref90_id": "UniRef90_Q9ZB78", "product": "Uncharacterized protein MG218.1", "uniref50_id": "UniRef50_Q9ZB78", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9ZB78", "UniRef:UniRef50_Q9ZB78" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZB78", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZB78" ], "product": "Uncharacterized protein MG218.1" } }, "id": "KMADJIBOEG_510", "locus": "GCA000292445_00483" }, { "type": "cds", "sequence": "contig_1", "start": 264850, "stop": 265161, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D5328C", "UniRef:UniRef90_UPI0002D5328C" ], "nt": "ATGGAAAATGAATTGGAAGCTGCTTTATTAGCAAAACACCAAGAAGAACCACCGTTAATTGTTACCCCACCCAGCTTAATAAAACCAACTGTTAGTCAACCTGAAGTTGAAGTTGTTACACCTACTAACAACACTAATTTCCAACCCCAAGTTGATCTCAAACCTACTGATTTGAAAAAACAACAGAAGAAAAAACCACTTAACTTTATTACCCGTCCTGTTTTCAAAAGTAATTTGCCACCGAAACTAAGTAAGGATGACATAGTTCATTATGCGCACCAGTTACTTGAAAAAAATACCCATAATGAATAG", "aa": "MENELEAALLAKHQEEPPLIVTPPSLIKPTVSQPEVEVVTPTNNTNFQPQVDLKPTDLKKQQKKKPLNFITRPVFKSNLPPKLSKDDIVHYAHQLLEKNTHNE", "aa_hexdigest": "4163eae9060dcff9184c604758f2730c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 264766, "stop": 265161, "observations": { "mutation": [ 264814 ], "stop": [ 264814 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0002D5328C", "query_cov": 1.0, "subject_cov": 0.7923076923076923, "identity": 0.971, "score": 189.0, "evalue": 2.16e-60, "gene_start": 1, "gene_end": 103, "reference_start": 29, "reference_end": 130, "reference_sequence": "MFGPPDIANDHELNLNWPDSETDAILSSMENELEAALLAKHQEPPLIVTLPSLIKPTVSQPEVEVVTPTNNTNFQPQVDLKPTDLKKQHKKKPLNFITRPVFKSNLPPKLSKDDIVHYAHQLLEKNTHNE" }, "paralog": false, "identity": 0.9694656488549618, "score": 253.0, "evalue": 5.61e-88, "description": "Internal stop codon at 264814. Nonsense mutation around 264814.", "psc": { "uniref90_id": "UniRef90_UPI0002D5328C", "uniref50_id": "UniRef50_UPI0002D5328C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0002D5328C", "UniRef:UniRef50_UPI0002D5328C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0002D5328C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D5328C" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11736.33639999999, "isoelectric_point": 6.913292503356933 }, "id": "KMADJIBOEG_511", "locus": "GCA000292445_00484" }, { "type": "cds", "sequence": "contig_1", "start": 265121, "stop": 265642, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MG219", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47461", "UniRef:UniRef90_UPI00027B3BDF" ], "nt": "ATGCGCACCAGTTACTTGAAAAAAATACCCATAATGAATAGTGATAGTGATCTAAAACTCCAAAAGGTGTGGATCGAGCGGCATGTTGATCAAGATGAACTTAGTTTAACAACTACTGCAGTTGAACTTAAAAAGAGTGATGAACAAAAACCTGTTGCCATTAAAAGTAGTGACTTTATTGGCCATGAAGAATTAATCTCTGTTCCAGTTTTACTAATCCCAACCCCTGTTGTTAAAGAGATTGACCAACCAGTAGTTATTACTCCAGTTAAAGCAAAACCAAAAGCAACTAAAAAGAAAACTCCTGTTAAATCAAAACCAACTAATAAATCAACTAAACAAACAAAACCTAAACAACCCAAACCCAAATCAAAACAAGTTCAAAAAACCAAAGCTAAACCAACCCCAAATTCAAACAAAAAAAGCATAAAAAAACCAGATCTTAATCTGGTTTTTTTAGTGTTAACAACAGTAGTTTTAACTAAAAACTGGAAAGGAATAGGAAGAACTACCTGGGGTTAA", "aa": "MRTSYLKKIPIMNSDSDLKLQKVWIERHVDQDELSLTTTAVELKKSDEQKPVAIKSSDFIGHEELISVPVLLIPTPVVKEIDQPVVITPVKAKPKATKKKTPVKSKPTNKSTKQTKPKQPKPKSKQVQKTKAKPTPNSNKKSIKKPDLNLVFLVLTTVVLTKNWKGIGRTTWG", "aa_hexdigest": "58889c7fbb109d746569df19ed0b4881", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00027B3BDF", "query_cov": 0.9364161849710982, "subject_cov": 1.0, "identity": 1.0, "score": 248.0, "evalue": 7.18e-82, "valid": true, "uniref50_id": "UniRef50_P47461" }, "pscc": { "uniref50_id": "UniRef50_P47461", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47461" ], "product": "Uncharacterized protein MG219" }, "genes": [], "id": "KMADJIBOEG_512", "locus": "GCA000292445_00485" }, { "type": "cds", "sequence": "contig_1", "start": 265598, "stop": 265801, "strand": "-", "frame": 3, "gene": null, "product": "MPN313 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_Q49403", "UniRef:UniRef90_Q49403" ], "nt": "ATGATAGTTAATGCACTTACTGGGGTTGCACATGCAGTAAATGACATTTTTCAAAGTGCCACAGCTAATGCCAATGGTTCTGATGATGATAATGAGAATAAAAATAATAGTTATCGCTCTAAAAGCAATTATTTTAACACCGCACGGTTTAAACTAGGATTAACCCCAGGTAGTTCTTCCTATTCCTTTCCAGTTTTTAGTTAA", "aa": "MIVNALTGVAHAVNDIFQSATANANGSDDDNENKNNSYRSKSNYFNTARFKLGLTPGSSSYSFPVFS", "aa_hexdigest": "3bcb727f9c0c069690c82c835a7c1130", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 265598, "stop": 265879, "observations": { "mutation": [ 265825 ], "stop": [ 265825 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49403", "query_cov": 1.0, "subject_cov": 0.7204301075268817, "identity": 0.97, "score": 103.0, "evalue": 2.19e-27, "gene_start": 1, "gene_end": 67, "reference_start": 27, "reference_end": 93, "reference_sequence": "MYKLEKAQAKQVVGGLSFWTFSAGLIMIVNALTGVAHAVNDIFQSTTANANGSDDDNENKNNSYRSKSNYFNTARFKLGLTPGSSSYSFPVFS" }, "paralog": false, "identity": 0.967741935483871, "score": 150.0, "evalue": 6.71e-49, "description": "Internal stop codon at 265825. Nonsense mutation around 265825.", "psc": { "uniref90_id": "UniRef90_Q49403", "product": "MPN313 family protein", "uniref50_id": "UniRef50_Q49403", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49403", "UniRef:UniRef50_Q49403", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q49403", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49403" ], "product": "Uncharacterized protein MG220" } }, "id": "KMADJIBOEG_513", "locus": "GCA000292445_00486" }, { "type": "tRNA", "sequence": "contig_1", "start": 265946, "stop": 266022, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(gcg)", "amino_acid": "Arg", "anti_codon": "gcg", "score": 65.2, "nt": "GTCATCATAGCTCAATAGGACAGAGTATCAGCTTGCGGAGCTGAGGGTTACAGGTTCGATTCCTGTTGGTGACGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 265980, 265982 ], "id": "KMADJIBOEG_9", "locus": "GCA000292445_00487" }, { "type": "cds", "sequence": "contig_1", "start": 266150, "stop": 266614, "strand": "+", "frame": 2, "gene": "mraZ", "product": "division/cell wall cluster transcriptional repressor MraZ", "db_xrefs": [ "COG:COG2001", "COG:J", "GO:0000976", "GO:0003700", "GO:0005737", "GO:0006355", "GO:0009295", "GO:2000143", "SO:0001217", "UniRef:UniRef50_P47463", "UniRef:UniRef90_P47463" ], "nt": "GTGTTTAAAAGTGTTGCAAAGTGTGACAAAGTGGAAAAAATGCTGCTAGGTACCTTTAATCTTACCCTTGATAACAAGAACAGAATTAGCTTGCCAGCTAAGCTCCGTAGTTTCTTTGATAGCAGCATAGTTATTAATCGCGGCTTTGAAAACTGTTTGGAAATTAGAAAACCTGCAGACTTTGAGAGTTATTTTCAAACCTTTAATAACTTCCCTAACACCCAAAAAGACACAAGAACATTAAAACGCTTAATCTTTGCTAATGCTAATCTAGTTGAACTTGATAGTGCAAACAGAATCCTAATCCCTAATAACTTAATTAGTGATGCTAAGTTAGATAAAGAGATCGTGTTAATTGGTCAATTTGATCACCTTGAAGTTTGGGATAAAGTGCAATATGAACAATATCTAGCTAGTTCAGAATCACTAGAGACAGTAGCTGAAAGGATGAAAGATGCTAAATAA", "aa": "MFKSVAKCDKVEKMLLGTFNLTLDNKNRISLPAKLRSFFDSSIVINRGFENCLEIRKPADFESYFQTFNNFPNTQKDTRTLKRLIFANANLVELDSANRILIPNNLISDAKLDKEIVLIGQFDHLEVWDKVQYEQYLASSESLETVAERMKDAK", "aa_hexdigest": "e5c898d6258936bf40e8c63458aea833", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47463", "query_cov": 0.9155844155844156, "subject_cov": 1.0, "identity": 1.0, "score": 276.0, "evalue": 8.68e-94, "valid": true, "gene": "mraZ", "product": "division/cell wall cluster transcriptional repressor MraZ", "uniref50_id": "UniRef50_P47463", "cog_id": "COG2001", "cog_category": "J", "go_ids": [ "GO:0000976", "GO:0003700", "GO:0005737", "GO:0006355", "GO:0009295", "GO:2000143" ] }, "pscc": { "uniref50_id": "UniRef50_P47463", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47463" ], "product": "Transcriptional regulator MraZ" }, "genes": [ "mraZ" ], "id": "KMADJIBOEG_514", "locus": "GCA000292445_00488" }, { "type": "cds", "sequence": "contig_1", "start": 266604, "stop": 267533, "strand": "+", "frame": 3, "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "db_xrefs": [ "COG:COG0275", "COG:J", "EC:2.1.1.199", "GO:0005737", "GO:0016434", "GO:0070475", "GO:0071424", "RefSeq:WP_014894227.1", "SO:0001217", "UniParc:UPI00027B3BE1", "UniRef:UniRef100_UPI00027B3BE1", "UniRef:UniRef50_P47464", "UniRef:UniRef90_P47464" ], "nt": "ATGCTAAATAACCAACAGATTCACCAGAGTGTACTGATCAATGAAGTGATCTATTACCTTAATATTAACCCTTGTGGTAACTATTTAGATCTAACTGCAGGGTTTGCAGGACACAGTCAAAAGATCTTAGAAAAACTAACAACGGGAACTTTAACAATTAATGATGTTGATAAAGAAAGTATTAATTTTTGCCAAAAGCTTTTTTTCAAAAACAACAATGTTGTTATTATTCACGATAACTTTGCTAACTTCCCAGTCCATCTTAAACAATTATCAATAACCAAGTTTGATGGGATCTTAATGGACCTTGGTGTATCAAGCCACCAACTCAACCAACCTAATCGAGGTTTTAGTTTTAAGAATGATGGACCGATTGACATGCGCATGGACCAATCCAATCAGAAAAATACCGCACTAATAGTTCTAAAAAACTTAACTGAACAAAAGTTAAGTCTAATCCTTAAAAAGTATGGTGATATTAAACACCCTAAACCAATTGCTATTGGACTGAAAAAAGCAGTTCAAACTGAAAAAAATCTTACCACAACTCAACTAGCAAAAGTGGTAAAAGAATGTGCTACTGGATTTGAAAAATACCAATCAAGAAACTATCTTGCTAAAGTTTTTCAAGCAATTAGGATCTATCTTAATGATGAGATTACTAATCTGAAAACTGCGTTAACTTTTATCCCTAATCTTTTAAAAAACAACAGCAGGTTTCTTGTGATTGTTTTTCACTCCATTGAAGAAAAAGTTGTAAGGAATTTCATTGCAAAACTAACCAGTTTTATCCAACCTGAAGCTCTACCCATTAAACTCACTCCTGCTTACCAGTTAATTACAAAAAAACCAATCCTACCTTCCCAAAAAGAACTTGAATTAAACCCGCGTTCGCGTAGTGCCAAACTCTTTGTTATCCAAAAAAACTAG", "aa": "MLNNQQIHQSVLINEVIYYLNINPCGNYLDLTAGFAGHSQKILEKLTTGTLTINDVDKESINFCQKLFFKNNNVVIIHDNFANFPVHLKQLSITKFDGILMDLGVSSHQLNQPNRGFSFKNDGPIDMRMDQSNQKNTALIVLKNLTEQKLSLILKKYGDIKHPKPIAIGLKKAVQTEKNLTTTQLAKVVKECATGFEKYQSRNYLAKVFQAIRIYLNDEITNLKTALTFIPNLLKNNSRFLVIVFHSIEEKVVRNFIAKLTSFIQPEALPIKLTPAYQLITKKPILPSQKELELNPRSRSAKLFVIQKN", "aa_hexdigest": "da6cec37f9d6df2cc1d1d0811e368c97", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3BE1", "ncbi_nrp_id": "WP_014894227.1", "uniref100_id": "UniRef100_UPI00027B3BE1", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3BE1", "RefSeq:WP_014894227.1", "UniRef:UniRef100_UPI00027B3BE1" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3BE1", "uniref90_id": "UniRef90_P47464" }, "psc": { "uniref90_id": "UniRef90_P47464", "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "ec_ids": [ "2.1.1.199" ], "uniref50_id": "UniRef50_P47464", "cog_id": "COG0275", "cog_category": "J", "go_ids": [ "GO:0005737", "GO:0016434", "GO:0070475", "GO:0071424" ] }, "pscc": { "uniref50_id": "UniRef50_P47464", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47464" ], "product": "Ribosomal RNA small subunit methyltransferase H" }, "genes": [ "rsmH" ], "id": "KMADJIBOEG_515", "locus": "GCA000292445_00489" }, { "type": "cds", "sequence": "contig_1", "start": 267535, "stop": 268722, "strand": "+", "frame": 1, "gene": null, "product": "MPN316 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47465", "UniRef:UniRef90_P47465" ], "nt": "ATGTACAAACCAAAAAATATTAACAGCGTATTAACCTTTTATAAGGATCAGATCCAACTGGTTGTTAGTGATGATCAAAACCAGTTCAACATCTTGTTTTACCAAACAATTGATAACGATGGCTTTTATTCAAAACAACAGTTGAAAAACAAACTAAGACTCAAGTTAGCATTAAACCAACTAGTTGATCAAGCTAACTATTTTCTTGGTTTTAAACTGGAAAAGGTAGTTGTTGTTCTCGCTGAACTGATTGATGATTTGAAGATCCATAATTTCAAGAGTGAGATCTTTTTTACTGGTTATGATTTTGATCATAAAGCGATGATTAAAAAAGAAAAACAACGCTTTTGTGAGCAAAATAACCAACTAACAGTTATGGATACAATGGTTTTAAACTACCATGATGTTATTAACAATAAGATCACCAAAAGCTTTGCATTTAACAAGAGCTATGTAGCTAATTTAGTGGCATATTCCTCTAAAAGTAACCTGATCGGGGAGTTGAAGTTCTTTTTAAAAAGAAACGTTAATCTTAAGGTTAAGAAAATTATTAGTCACCACTTAGCATTAGCCAACTCCTTAAGTAAGAAACAAAACAACATGTTTGTTTATTTAGGACAAAAAACTACTGAACTGATGCTATTTATGGACAATGCTTTAGTTGATGTTATTACCAACCAGTTTGGTAAAAACCACTTTATTGATATTCCAGCTAACCAGGAAAACAAACCACTGCTTGAGTTTTTAGTTGATAACACCACTAAGATTGGTGATTGTTATTCGCTTGGTATGACCTATACAGATGGTGATAGTTACAAAGAGATTAAGGCTTTGACTATTGGTGATTTAATGCAAACAGTTAGTGACAAGATCAAAACCTTAATTGATTTTATTAACAGTGGTTCTCTAACTTTTTTCAACAAGTTTAAAACCTTACCTAAGCTATTGTATTTTTATACAAGATCAAAACAAATTACCAACCTTTTTCAAGCTAATGTTGCACTTATCAATCCCCAGTTTAAAACTGTTGATATTTATAAGAACAAGATCCAGTTTATTAGTGAAAACTACCTGTTAAGCTGTGAAGCGATTAGCTTGCAGATTACCAATAGAATCAAAAACCAAATTAGTTTTGATTTCACAAATGCTGATAATATTCAAAAACCTAAACCAAAAAACACTTCATGA", "aa": "MYKPKNINSVLTFYKDQIQLVVSDDQNQFNILFYQTIDNDGFYSKQQLKNKLRLKLALNQLVDQANYFLGFKLEKVVVVLAELIDDLKIHNFKSEIFFTGYDFDHKAMIKKEKQRFCEQNNQLTVMDTMVLNYHDVINNKITKSFAFNKSYVANLVAYSSKSNLIGELKFFLKRNVNLKVKKIISHHLALANSLSKKQNNMFVYLGQKTTELMLFMDNALVDVITNQFGKNHFIDIPANQENKPLLEFLVDNTTKIGDCYSLGMTYTDGDSYKEIKALTIGDLMQTVSDKIKTLIDFINSGSLTFFNKFKTLPKLLYFYTRSKQITNLFQANVALINPQFKTVDIYKNKIQFISENYLLSCEAISLQITNRIKNQISFDFTNADNIQKPKPKNTS", "aa_hexdigest": "a23ab36b279c0ecb375a6c8a4925d694", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47465", "query_cov": 0.9924050632911392, "subject_cov": 0.9537712895377128, "identity": 1.0, "score": 771.0, "evalue": 3.7e-281, "valid": true, "product": "MPN316 family protein", "uniref50_id": "UniRef50_P47465" }, "pscc": { "uniref50_id": "UniRef50_P47465", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47465" ], "product": "Uncharacterized protein MG223" }, "genes": [], "id": "KMADJIBOEG_516", "locus": "GCA000292445_00490" }, { "type": "cds", "sequence": "contig_1", "start": 268772, "stop": 269662, "strand": "+", "frame": 2, "gene": "ftsZ", "product": "cell division protein FtsZ", "db_xrefs": [ "COG:COG0206", "COG:D", "GO:0000910", "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0032153", "GO:0043093", "GO:0051258", "GO:0051301", "KEGG:K03531", "SO:0001217", "UniRef:UniRef50_P47466", "UniRef:UniRef90_P47466" ], "nt": "ATGGATGAAAATGAAACTCAATTCAACAAGTTAAACCAAGTTAAAAACAAGCTGAAAATTGGTGTTTTTGGGATTGGAGGTGCTGGTAATAACATTGTTGATGCATCACTTTATCACTATCCTAATTTAGCAAGTGAAAACATCCACTTTTATGCTATAAATTCAGATTTACAACACCTTGCATTTAAAACGAATGTTAAAAATAAACTCTTAATTCAAGACCATACTAACAAGGGCTTTGGAGCGGGGGGTGATCCAGCTAAAGGAGCTAGTTTAGCAATAAGCTTTCAAGAACAGTTTAATACACTTACAGATGGGTATGATTTTTGTATCTTAGTTGCTGGATTTGGTAAGGGTACTGGTACAGGTGCTACCCCAGTTTTTAGCAAGATCTTAAAAACTAAGAAGATCTTAAATGTTGCTATTGTTACCTATCCATCTTTAAACGAGGGATTAACAGTGAGAAACAAAGCCACTAAGGGGCTTGAAATTCTCAACAAAGCAACTGATAGTTACATGCTATTTTGTAATGAAAAATGTACAAATGGTATCTACCAACTAGCAAACACAGAGATAGTCAGTGCCATTAAAAACCTAATAGAACTAATTACTATTCCTTTGCAGCAAAACATTGATTTTGAAGATGTACGTGCCTTTTTTCAAACCAAAAAAACTAACCAAGATCAACAGCTTTTTACTGTTACTCACCCCTTTAGTTTTAGCTTTGATAGTAAAGATAGTATAGAACAGTTTGCTAAACAGTTTAAGAACTTTGAAAAAGTTAGTTATTTTGACCACTCTATAGTAGGAGCTAAAAAAGTGTTATTGAAAGCTAACATTAACCAAAAGATAGTCAAGCTTAACTTCAAGCAGATCCAAGATATTATCTGA", "aa": "MDENETQFNKLNQVKNKLKIGVFGIGGAGNNIVDASLYHYPNLASENIHFYAINSDLQHLAFKTNVKNKLLIQDHTNKGFGAGGDPAKGASLAISFQEQFNTLTDGYDFCILVAGFGKGTGTGATPVFSKILKTKKILNVAIVTYPSLNEGLTVRNKATKGLEILNKATDSYMLFCNEKCTNGIYQLANTEIVSAIKNLIELITIPLQQNIDFEDVRAFFQTKKTNQDQQLFTVTHPFSFSFDSKDSIEQFAKQFKNFEKVSYFDHSIVGAKKVLLKANINQKIVKLNFKQIQDII", "aa_hexdigest": "af84eeb795d1c95ddba768062f8d4188", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47466", "query_cov": 1.0, "subject_cov": 0.8021680216802168, "identity": 1.0, "score": 566.0, "evalue": 1.65e-202, "valid": true, "gene": "ftsZ", "product": "cell division protein FtsZ", "uniref50_id": "UniRef50_P47466", "cog_id": "COG0206", "cog_category": "D", "kegg_orthology_id": "K03531", "go_ids": [ "GO:0000910", "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0032153", "GO:0043093", "GO:0051258", "GO:0051301" ] }, "pscc": { "uniref50_id": "UniRef50_P47466", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47466" ], "product": "Cell division protein FtsZ" }, "genes": [ "ftsZ" ], "id": "KMADJIBOEG_517", "locus": "GCA000292445_00491" }, { "type": "cds", "sequence": "contig_1", "start": 269711, "stop": 269881, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACAACCATCCCTAATATCCAAATTTTTATCCTCAGTGAACACAAAAATCCAGTTTCGCTTCCCATTGATAATAAATCAACTGAAAACAACCAAAATAAGTTGAAACTTTTAGATGAGCTGAAAGAACTTGGCATGAAATATGTTAAGCACCAAAACCAAATCTACTAA", "aa": "MTTIPNIQIFILSEHKNPVSLPIDNKSTENNQNKLKLLDELKELGMKYVKHQNQIY", "aa_hexdigest": "597266b74abfa706df615d518737eda1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6565.529300000002, "isoelectric_point": 8.050183296203613 }, "id": "KMADJIBOEG_518", "locus": "GCA000292445_00492" }, { "type": "cds", "sequence": "contig_1", "start": 269927, "stop": 270214, "strand": "+", "frame": 2, "gene": null, "product": "amino acid permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027DFE8A8" ], "nt": "ATGGGTCAAATCAATCGGAAGTTTAGCGAAAAGCAGTTCTTACTTTTTGTTGTTAACTATATTGCTGGATTTGGCTTTATTGCTACTGCTATCTCACTGTTTCGCTTAGGACCTTTTTCTTGGTTAATCTTTCTGCTTGTTAGCTTAGTGAGTTTAATTGTTACCTTATCATTCGCACGGCTTTCATCAATAGATAGTCAAAACTATGGTGGGCCTTATCTTTGGGCTAAGAAAGCGGTTGATAAAGAGAAGATAGCAGGGAGAATGTTTAGCTTTTTTACTGGGTGA", "aa": "MGQINRKFSEKQFLLFVVNYIAGFGFIATAISLFRLGPFSWLIFLLVSLVSLIVTLSFARLSSIDSQNYGGPYLWAKKAVDKEKIAGRMFSFFTG", "aa_hexdigest": "9aa49a3a7eaf04b55bdcdf088691fde6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0027DFE8A8", "query_cov": 0.9263157894736842, "subject_cov": 1.0, "identity": 0.67, "score": 122.0, "evalue": 1.42e-34, "valid": false, "product": "amino acid permease", "uniref50_id": "UniRef50_UPI0027DFE8A8" }, "pscc": { "uniref50_id": "UniRef50_UPI0027DFE8A8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027DFE8A8" ], "product": "amino acid permease" }, "genes": [], "id": "KMADJIBOEG_519", "locus": "GCA000292445_00493" }, { "type": "cds", "sequence": "contig_1", "start": 270142, "stop": 270315, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAACTAAGTTACCTCTAATCCCATCAATACCACTAAAGGAATTGAGGATAAAAAGTGGTGCAGTTGCTGCTGAAAGGGGACCAATGATAAAGTTATTTCACCCAGTAAAAAAGCTAAACATTCTCCCTGCTATCTTCTCTTTATCAACCGCTTTCTTAGCCCAAAGATAA", "aa": "MLTKLPLIPSIPLKELRIKSGAVAAERGPMIKLFHPVKKLNILPAIFSLSTAFLAQR", "aa_hexdigest": "dc4015c7b6b0a5f0d0550e2cd38aeccb", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6279.680499999999, "isoelectric_point": 11.165940284729004 }, "id": "KMADJIBOEG_520", "locus": "GCA000292445_00494" }, { "type": "cds", "sequence": "contig_1", "start": 270419, "stop": 271111, "strand": "+", "frame": 2, "gene": "potE", "product": "putative protein MG225", "db_xrefs": [ "COG:COG0531", "COG:E", "GO:0005886", "GO:0022857", "SO:0001217", "UniRef:UniRef50_P47467", "UniRef:UniRef90_P47467" ], "nt": "GTGAAGTGGATTGTGATCCTCTCAGCACTAATAGTAGCAATCTATGTTATTGCTAGAGATGGTAATGGTTATAGTCAAAACAATAACTTAGAAAGTGGTTTTTTTGGGAGAAGAGAGATTAGTTTTGCACAGATAGCAACGGTATTTATTACCTTCTTTTATTCTTATGCAGGGGTTGAAGATATCTCAGTGATGACTCCTGATGTTAAAACTAATAACTTTAGAAAGATATTAATTGTCTCTTTTATAGCAGTTTTCCTCTTTTATTTCATTGGGATTATTATTCTAAATGGTTTGCAAAACATTGCTCAAAGAGGTGGGGAAGCCAATTCAATTGGTAATGTAGCGGATATCTTTAAAAAAGCTGCTGGGCTTGGGACTTTAATCTTTTATGGAGTTGGAGCATTGTTTAACAATGTCTCAACCAGACTTTCAACTATTATTGCCAACTCCAGAAAGATTCTTCCGCTTGCTTATGATAACTATTTACCTAGTTTCTTTTACAAGCAAAACAAAAAAGGTGAGTTTCAGAATGCAATTTGGTTTACCTTTGGTACTACTTTAATTGCAATGACTTTGCTTGTCTTTATCCCTTTAGTTGCTTCTAACTTTGATTTTGATAATGCTACTGAGTATGCAGCATCTGTTGGCTCAGCTGCAACTTTGCTACAATATATTTTTGTTTTTTTATAA", "aa": "MKWIVILSALIVAIYVIARDGNGYSQNNNLESGFFGRREISFAQIATVFITFFYSYAGVEDISVMTPDVKTNNFRKILIVSFIAVFLFYFIGIIILNGLQNIAQRGGEANSIGNVADIFKKAAGLGTLIFYGVGALFNNVSTRLSTIIANSRKILPLAYDNYLPSFFYKQNKKGEFQNAIWFTFGTTLIAMTLLVFIPLVASNFDFDNATEYAASVGSAATLLQYIFVFL", "aa_hexdigest": "54c499a0700fc1d86e641a8f7f819a0e", "start_type": "GTG", "rbs_motif": null, "genes": [ "potE" ], "truncated": "both", "pseudogene": { "start": 270119, "stop": 271395, "observations": { "deletion": [ 271103 ], "mutation": [ 270212, 270317 ], "stop": [ 270212, 270317 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47467", "query_cov": 0.991304347826087, "subject_cov": 0.4662576687116564, "identity": 1.0, "score": 439.0, "evalue": 1.68e-151, "gene_start": 2, "gene_end": 229, "reference_start": 166, "reference_end": 393, "reference_sequence": "MGQINRKFSEKQFLLFVVNYIAGFGFIATAISLFRLGPFSWLIFLLVSLVSLIVTLSFARLSSIDSQNYGGPYLWAKKAVDKEKIAGRMFSFFTGWNNFIIGPLSAATAPLFILNSFSGIDGIRGNLVNTWILIAIGFSFYVLLAFISTKGTSLNKKLIALFASVKWIVILSALIVAIYVIARDGNGYSQNNNLESGFFGRREISFAQIATVFITFFYSYAGVEDISVMTPDVKTNNFRKILIVSFIAVFLFYFIGIIILNGLQNIAQRGGEANSIGNVADIFKKAAGLGTLIFYGVGALFNNVSTRLSTIIANSRKILPLAYDNYLPSFFYKQNKKGEFQNAIWFTFGTTLIAMTLLVFIPLVASNFDFDNATEYAASVGSAATLLQYIFVFFIIFKFIYKKEPLYQKKWVKTTEELLFCLGTIVIVLMLLVYLFPVIDGFSKWETKHTLTIVLYGVLSLIGLVLFLLQEYKHKNKQNANKQTTQTTV" }, "paralog": false, "identity": 0.9952941176470588, "score": 820.0, "evalue": 3.16e-304, "description": "Internal stop codon at 270212, 270317. Frameshift due to deletion around 271103. Nonsense mutation around 270212, 270317.", "psc": { "uniref90_id": "UniRef90_P47467", "gene": "PotE", "product": "Uncharacterized protein MG225", "uniref50_id": "UniRef50_P47467", "cog_id": "COG0531", "cog_category": "E", "go_ids": [ "GO:0005886", "GO:0022857" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47467", "UniRef:UniRef50_P47467", "COG:COG0531", "COG:E", "GO:0005886", "GO:0022857" ] }, "pscc": { "uniref50_id": "UniRef50_P47467", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47467" ], "product": "Uncharacterized protein MG225" } }, "id": "KMADJIBOEG_521", "locus": "GCA000292445_00495" }, { "type": "cds", "sequence": "contig_1", "start": 271180, "stop": 271395, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTTGTTTAGGAACAATTGTCATTGTTTTAATGTTGTTGGTTTATCTGTTTCCTGTTATAGATGGATTTTCAAAATGGGAAACTAAACACACATTAACAATTGTGTTATATGGGGTTTTAAGCCTGATAGGATTGGTACTTTTTTTGCTCCAAGAATACAAACATAAAAACAAGCAAAATGCAAACAAACAAACAACCCAAACAACAGTTTAG", "aa": "MFCLGTIVIVLMLLVYLFPVIDGFSKWETKHTLTIVLYGVLSLIGLVLFLLQEYKHKNKQNANKQTTQTTV", "aa_hexdigest": "b9aa06906a031d704aca502ef5d5018a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8128.696199999995, "isoelectric_point": 9.252454185485838 }, "id": "KMADJIBOEG_522", "locus": "GCA000292445_00496" }, { "type": "cds", "sequence": "contig_1", "start": 271611, "stop": 272636, "strand": "+", "frame": 3, "gene": null, "product": "APC family permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EE64A6D", "UniRef:UniRef90_UPI001EE64A6D" ], "nt": "TTGGTACTAACAGTAAAACAAAAAGGTTTTTGTTTTTTAAGTGGGTGGAATGTATCGTTAACAGGATCTTTTAACGGTGTTGTTATTCCAGCAGTATTAATCTTTTCCTTTGCAGATATTCCAGTAGTTAAAGCGAATAATAACATCATTATTGGTCTTTTAGTAGGTGGATTTTTGTTGTTTGGCTTACTTACTTTTATCTCGTTATTTGGTTTAAAAATTAACAAGAAAGCAATCTTTTATTTTGCTGTTATTAAGTGGATAGTAGTAATAGGTGGGTTTATCTTAGGGATCTATTTAATTGGTACTACCAATGGTAAAGGGTTTGTTGAAAACAATTTAATTGGGACTAGGGAAAACATTGATTTTTTCAAGATTATCTTTATTAGTCTGGCTTTAACTATTGCTTTTGCAGGGACAGAGGATTTAGCTTCGATTACTCCTGATGTCAAGTCAAATAACTTAAGAAAGTGTTTTTTAATTGCCTTTGGGTGTGTTGTGTTACTTTACCTAGTTGGGTTTGTTATTATCAGTGGACTTGATGGGATTAGAGGTTATGGATTAGCATTAGGTAATAAAGATCCCAAGGCAATTAATAACTATGGATCTATCTACCGTTTGGTAGGAGGAGTTCCTTTACTTGTTATCTATGGACTTGGGTTACTTGTCAATTCCTTAGCATCACGCTTATCAATGACAATTACAACAGCTAGAAAATATGTAGCTTTAGCTCAAGATGGGTTTTTACCCTCTTTTTTAGCCAAAACTAATAAACATAATGAGTATCACCATGCAGTTTTAATTAGTAATCTAATGACTTTATTAGTGATGCTAATTATGGTAATAATCCCCTTTTTACCAGACCATAACAACAATAACAATAGTTTGTTTAATGCTATTGAACAGTTGGTTACAGTTACCATTGAAATGGCTGCAGCCATTTCTTTGATCCAATACTTTATTACCTTTATCTTCTTTTTTATGATCTTCGCTAAAAAGGAAAACCAGAAGTTAATTCCCTTGTGA", "aa": "MVLTVKQKGFCFLSGWNVSLTGSFNGVVIPAVLIFSFADIPVVKANNNIIIGLLVGGFLLFGLLTFISLFGLKINKKAIFYFAVIKWIVVIGGFILGIYLIGTTNGKGFVENNLIGTRENIDFFKIIFISLALTIAFAGTEDLASITPDVKSNNLRKCFLIAFGCVVLLYLVGFVIISGLDGIRGYGLALGNKDPKAINNYGSIYRLVGGVPLLVIYGLGLLVNSLASRLSMTITTARKYVALAQDGFLPSFLAKTNKHNEYHHAVLISNLMTLLVMLIMVIIPFLPDHNNNNNSLFNAIEQLVTVTIEMAAAISLIQYFITFIFFFMIFAKKENQKLIPL", "aa_hexdigest": "6583ff1b9c2c11a3f3997678d824b39c", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001EE64A6D", "query_cov": 0.9178885630498533, "subject_cov": 0.8236842105263158, "identity": 0.997, "score": 543.0, "evalue": 2.02e-192, "valid": true, "product": "APC family permease", "uniref50_id": "UniRef50_UPI001EE64A6D" }, "pscc": { "uniref50_id": "UniRef50_UPI001EE64A6D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EE64A6D" ], "product": "APC family permease" }, "genes": [], "id": "KMADJIBOEG_523", "locus": "GCA000292445_00497" }, { "type": "cds", "sequence": "contig_1", "start": 272817, "stop": 272954, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013052C28" ], "nt": "ATGTTAGAAAAAGTGGGGAGTGGGGTAGAAGAAGAAGAAGAAGAAGAAGGGGAGGGGGAGTTGGAGAAGGGTTTACCATTGCCGATTCAAAAGCAATTTCGCTTAATGAACTGTAGATTAGCTATTACTATTCATTAA", "aa": "MLEKVGSGVEEEEEEEGEGELEKGLPLPIQKQFRLMNCRLAITIH", "aa_hexdigest": "e0d77f5b932b87228af0bec1c0ab1e41", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0013052C28", "query_cov": 1.0, "subject_cov": 0.8333333333333334, "identity": 0.667, "score": 58.2, "evalue": 2.17e-10, "valid": false, "uniref50_id": "UniRef50_UPI0013052C28" }, "pscc": { "uniref50_id": "UniRef50_UPI0013052C28", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013052C28" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5110.793, "isoelectric_point": 4.537934303283691 }, "id": "KMADJIBOEG_524", "locus": "GCA000292445_00498" }, { "type": "cds", "sequence": "contig_1", "start": 272982, "stop": 273218, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGTTTGGTAAAGCATCATACGTTGTTTTTTTGGCTGGGGAGCTACCTGATGGTGTGACTTGGTTTTCAGAAGAATTGGGATTAAAGGGGGTACTACTATTGCTAGGCTTCACCATTTCAAAGGAGATACCGTTATTGTTGTCATTACCCTTGCGATACCCTGCTCCCGTTCAAATGTCAGGTGAAGAGTTAGGGGTAAACACCCACCGCGTGTTTTCTTTATTTAAATGTTGA", "aa": "MEFGKASYVVFLAGELPDGVTWFSEELGLKGVLLLLGFTISKEIPLLLSLPLRYPAPVQMSGEELGVNTHRVFSLFKC", "aa_hexdigest": "bcabeba0658c09dc79e1b5d6279d27f8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8601.058199999992, "isoelectric_point": 5.19482250213623 }, "id": "KMADJIBOEG_525", "locus": "GCA000292445_00499" }, { "type": "cds", "sequence": "contig_1", "start": 273310, "stop": 273492, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00303.24" ], "nt": "ATGAAACAGTATTTAGATTTAGCTAGTTATGTTTTAGCAAATGGTAAAAAAAGAAAAAACCGTACAGATACAGATACTTTAAGTGTCTTTGGTTACCAGATGAAATTTGACCTTACTAATAGTTTTCCTTTATTGACAACTAAAAAGGTTAATTGGAAGGCAATTGTCCATGAATTGTTGTGA", "aa": "MKQYLDLASYVLANGKKRKNRTDTDTLSVFGYQMKFDLTNSFPLLTTKKVNWKAIVHELL", "aa_hexdigest": "bb6b9ca0c6ac04d4b0d6b8e779825ef8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0037C09292", "query_cov": 1.0, "subject_cov": 0.9375, "identity": 0.6, "score": 75.1, "evalue": 1.01e-16, "valid": false, "product": "thymidylate synthase", "uniref50_id": "UniRef50_A0A967H814" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00303.24", "name": "Thymidylat_synt", "length": 60, "aa_cov": 0.9833333333333333, "hmm_cov": 1.0, "evalue": 7.505087597639964e-23, "score": 76.10872650146484, "start": 2, "stop": 60 } ], "seq_stats": { "molecular_weight": 6995.086499999999, "isoelectric_point": 9.755372047424316 }, "id": "KMADJIBOEG_526", "locus": "GCA000292445_00500" }, { "type": "cds", "sequence": "contig_1", "start": 273802, "stop": 274173, "strand": "+", "frame": 1, "gene": "thyA", "product": "thymidylate synthase", "db_xrefs": [ "COG:COG0207", "COG:F", "EC:2.1.1.45", "GO:0004799", "GO:0005829", "GO:0006231", "GO:0006235", "GO:0032259", "KEGG:K00560", "SO:0001217", "UniRef:UniRef50_P47469", "UniRef:UniRef90_P47469" ], "nt": "ATGGCATTGGCTCCTTGTCATTCACTCTTTCAGTTCTATGTTGAAGAAGATAAACTAAGCTTACAGCTTTACCAGCGCAGCGGTGATATCTTTCTTGGTGTCCCATTTAACATTGCATCTTACGCCTTACTTGTGTATTTAGTTGCTCATGAAACTAAGTTAAAACCTGGTTATTTTATCCATACACTAGGAGATGCACATATCTATGAAAACCACATTGAACAAATTAAATTACAACTAACAAGAACAACCCTAGACCCCCCTCAAGTGGTTTTGAAAAGTGATAAATCAATCTTTGCTTATAGTTTTGATGATATTGAGTTAGTTGGTTATAATTACCATCCATTTATCTATGGGAGGGTTGCAGTTTAA", "aa": "MALAPCHSLFQFYVEEDKLSLQLYQRSGDIFLGVPFNIASYALLVYLVAHETKLKPGYFIHTLGDAHIYENHIEQIKLQLTRTTLDPPQVVLKSDKSIFAYSFDDIELVGYNYHPFIYGRVAV", "aa_hexdigest": "45fc626ecca00e240ef3748f49293db2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00260828A0", "query_cov": 1.008130081300813, "subject_cov": 0.8104575163398693, "identity": 0.629, "score": 156.0, "evalue": 1.25e-46, "valid": false, "gene": "thyA", "product": "thymidylate synthase", "ec_ids": [ "2.1.1.45" ], "uniref50_id": "UniRef50_A0A059X7H5", "kegg_orthology_id": "K00560", "go_ids": [ "GO:0004799", "GO:0006231" ] }, "genes": [ "thyA" ], "truncated": "5-prime", "pseudogene": { "start": 273502, "stop": 274173, "observations": { "mutation": [ 273547, 273556, 273688, 273778 ], "stop": [ 273547, 273556, 273688, 273778 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47469", "query_cov": 1.0, "subject_cov": 0.42857142857142855, "identity": 1.0, "score": 254.0, "evalue": 2.18e-83, "gene_start": 1, "gene_end": 123, "reference_start": 165, "reference_end": 287, "reference_sequence": "MKQYLDLASYVLANGKKRKNRTDTDTLSVFGYQMKFDLTNSFPLLTTKKVNWKAIVHELLWFIKGDTNIKYLVDNGVNIWNEWPYENFKKSPSFQNETLQEFILKVKTDNEFAKQFADLGPVYGKQWRNFNGVDQLKKVIQEIKENPNSRRLIVSSWNPSELEKMALAPCHSLFQFYVEEDKLSLQLYQRSGDIFLGVPFNIASYALLVYLVAHETKLKPGYFIHTLGDAHIYENHIEQIKLQLTRTTLDPPQVVLKSDKSIFAYSFDDIELVGYNYHPFIYGRVAV" }, "paralog": false, "identity": 0.9820627802690582, "score": 437.0, "evalue": 4.37e-158, "description": "Internal stop codon at 273547, 273556, 273688, 273778. Nonsense mutation around 273547, 273556, 273688, 273778.", "psc": { "uniref90_id": "UniRef90_P47469", "gene": "thyA", "product": "thymidylate synthase", "ec_ids": [ "2.1.1.45" ], "uniref50_id": "UniRef50_P47469", "cog_id": "COG0207", "cog_category": "F", "kegg_orthology_id": "K00560", "go_ids": [ "GO:0004799", "GO:0005829", "GO:0006231", "GO:0006235", "GO:0032259" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47469", "EC:2.1.1.45", "UniRef:UniRef50_P47469", "COG:COG0207", "COG:F", "KEGG:K00560", "GO:0004799", "GO:0005829", "GO:0006231", "GO:0006235", "GO:0032259" ] }, "pscc": { "uniref50_id": "UniRef50_P47469", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47469" ], "product": "Thymidylate synthase" } }, "id": "KMADJIBOEG_527", "locus": "GCA000292445_00501" }, { "type": "cds", "sequence": "contig_1", "start": 274295, "stop": 274423, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00186.24" ], "nt": "ATGGGGAGAAAAACTTACGAATCACTCCCCAAGGTATTTGAAAAAAGAACAATATTTCTCCTTTCAAAAGATCAAAACTACCGTTTTGAAGAAAAGGGAAGTGAAGTGAAAGTTATTAATGATTTTTGA", "aa": "MGRKTYESLPKVFEKRTIFLLSKDQNYRFEEKGSEVKVINDF", "aa_hexdigest": "9026419b09021a19192b696842693f3e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00186.24", "name": "DHFR_1", "length": 31, "aa_cov": 0.7380952380952381, "hmm_cov": 1.0, "evalue": 2.2014801720234436e-08, "score": 29.10838508605957, "start": 1, "stop": 31 } ], "seq_stats": { "molecular_weight": 5071.7615000000005, "isoelectric_point": 9.156267356872558 }, "id": "KMADJIBOEG_528", "locus": "GCA000292445_00502" }, { "type": "cds", "sequence": "contig_1", "start": 274463, "stop": 274654, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00186.24" ], "nt": "TTGTTTATTTGTGGTGGAAAAAGTGTGTATGAACAGACCATTAATGAATGTGATCAGTTAATTGTTTCAATCATTAAAAAGAAGTATAAGGGTGATCAGTTTTTGAAGGTTGATCTCAGTAAATTTGTACTTAATGAAGTTGTAGAGTTTGAGGAATTTAATGTTAATTATTATAGAAAGAAACAACAATAA", "aa": "MFICGGKSVYEQTINECDQLIVSIIKKKYKGDQFLKVDLSKFVLNEVVEFEEFNVNYYRKKQQ", "aa_hexdigest": "158254ec2552fe2e19ebf56e2f50e11b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00186.24", "name": "DHFR_1", "length": 53, "aa_cov": 0.8253968253968254, "hmm_cov": 1.0, "evalue": 8.050260135414871e-10, "score": 33.77817916870117, "start": 1, "stop": 52 } ], "seq_stats": { "molecular_weight": 7516.623, "isoelectric_point": 7.714665412902832 }, "id": "KMADJIBOEG_529", "locus": "GCA000292445_00503" }, { "type": "cds", "sequence": "contig_1", "start": 274660, "stop": 274791, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGCTAACAATAAAAAGTACTTTTTAGAATCATTTTCCCCACTTGGGTATGTAAAGAATAATTTTCAGGGCAACTTACGTTCTGTAAACTGGAATTTGGTTGATGATGAGAAGGATTTGGAAGTGTGA", "aa": "MAANNKKYFLESFSPLGYVKNNFQGNLRSVNWNLVDDEKDLEV", "aa_hexdigest": "a7567c754e2a481a928e9a97b07baeff", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5008.533000000001, "isoelectric_point": 4.992987251281736 }, "id": "KMADJIBOEG_530", "locus": "GCA000292445_00504" }, { "type": "cds", "sequence": "contig_1", "start": 275011, "stop": 275682, "strand": "+", "frame": 1, "gene": null, "product": "Ribonucleoside-diphosphate reductase subunit beta", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2I1IMZ3" ], "nt": "ATGGTAGGGGTACATGCCCGTTCCTATGGAACGATCTTCTCAACTTTATGTACATCAGAACAGATTAACGCTGCTCATGAGTGGGTTGTAAACACTGAAAGTCTCCAGAAAAGAGCAAAGGCATTAATCCCTTACTATACGGGCAATGACCCGTTAAAATCAAAGGTAGCAGCAGCTTTAATGCCTGGGTTTTTACTGTATGGTGGGTTTTATTTGCCTTTTTACTTGTCATCAAGAAAACAACTACCAAATACATCTGATATTATCCGCTTAATCCTTCGTGATAAAGTGATCCATAACTATTACAGTGGTTATAAATACCAACGTAAACTAGAAAAACTCCCTTTAGCAAAACAAAAGGAGATGAAAGCATTTGTTTTTGAACTAATGTATCGGTTAATTGAACTTGAAAAGGACTATTTAAAAGAGCTTTATGAAGGGTTTGGAATTGTTGATGATGCCATTAAGTTCAGTGTTTACAATGCTGGTAAGTTTTTACAGAACTTAGGTTATGACTCCCCGTTTACTGCAGCAGAAACCAGGATTAAACCAGAGATTTTTGCCCAACTATCAGCACGTGCTGATGAAAACCATGACTTTTTCTCAGGAAACGGTTCGTCGTATGTGATGGGAGTTAGTGAAGAGACAAATGATGATGATTGGAACTTTTAA", "aa": "MVGVHARSYGTIFSTLCTSEQINAAHEWVVNTESLQKRAKALIPYYTGNDPLKSKVAAALMPGFLLYGGFYLPFYLSSRKQLPNTSDIIRLILRDKVIHNYYSGYKYQRKLEKLPLAKQKEMKAFVFELMYRLIELEKDYLKELYEGFGIVDDAIKFSVYNAGKFLQNLGYDSPFTAAETRIKPEIFAQLSARADENHDFFSGNGSSYVMGVSEETNDDDWNF", "aa_hexdigest": "8cc6ab481636ff6e2182b5b833251dc4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2X3CV73", "query_cov": 1.0, "subject_cov": 0.8383458646616542, "identity": 0.507, "score": 231.0, "evalue": 7.19e-73, "valid": false, "product": "ribonucleoside-diphosphate reductase", "uniref50_id": "UniRef50_A0A2I1IMZ3" }, "pscc": { "uniref50_id": "UniRef50_A0A2I1IMZ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2I1IMZ3" ], "product": "Ribonucleoside-diphosphate reductase subunit beta" }, "genes": [], "id": "KMADJIBOEG_531", "locus": "GCA000292445_00505" }, { "type": "cds", "sequence": "contig_1", "start": 275686, "stop": 276147, "strand": "+", "frame": 1, "gene": "nrdI", "product": "class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI", "db_xrefs": [ "COG:COG1780", "COG:F", "GO:0009263", "GO:0010181", "GO:0036211", "KEGG:K03647", "RefSeq:WP_014894231.1", "SO:0001217", "UniParc:UPI00027B389F", "UniRef:UniRef100_UPI00027B389F", "UniRef:UniRef50_P47472", "UniRef:UniRef90_P47472" ], "nt": "ATGCATAAAGATATCAAACTAGTTAAGGAAACTGAAATTAGAAAACCAATTGGTTCTCCTTTTATTGTCTATTTTTCATCTATCTCCAACAACACCCACCGTTTTATTGAAAAACTGGGTTTTCAACACAAAAGAATCCCAGTTGATATAACCCAAAGCATTACTATAAGTAATGAGTATGTTTTAATCTGTCCAACTTACAGTGGTGGGGGTAACCAGGTTGAAGGAGCGGTACCCAAGCAAGTTATCCAGTTTTTAAATAACAAGCATAACAGGGAGTTATGCAGAGGAGTTATTGCATCTGGTAACACTAATTTTGGAGATACTTTTTGTCTTGCAGGAACTGTTATTTCCAAAAAACTAAACGTCCCTTTGTTATATCAGTTTGAACTTTTGGGAACAAAAAATGATGTAGAACAAACTCAAAAAATAATTGCCAATTTCTTTCAAAACAGCAACTAG", "aa": "MHKDIKLVKETEIRKPIGSPFIVYFSSISNNTHRFIEKLGFQHKRIPVDITQSITISNEYVLICPTYSGGGNQVEGAVPKQVIQFLNNKHNRELCRGVIASGNTNFGDTFCLAGTVISKKLNVPLLYQFELLGTKNDVEQTQKIIANFFQNSN", "aa_hexdigest": "c3f79c7970add0d0004d7767c9a1bd86", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B389F", "ncbi_nrp_id": "WP_014894231.1", "uniref100_id": "UniRef100_UPI00027B389F", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B389F", "RefSeq:WP_014894231.1", "UniRef:UniRef100_UPI00027B389F" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B389F", "uniref90_id": "UniRef90_P47472" }, "psc": { "uniref90_id": "UniRef90_P47472", "gene": "nrdI", "product": "class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI", "uniref50_id": "UniRef50_P47472", "cog_id": "COG1780", "cog_category": "F", "kegg_orthology_id": "K03647", "go_ids": [ "GO:0009263", "GO:0010181", "GO:0036211" ] }, "pscc": { "uniref50_id": "UniRef50_P47472", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47472" ], "product": "Protein NrdI" }, "genes": [ "nrdI" ], "id": "KMADJIBOEG_532", "locus": "GCA000292445_00506" }, { "type": "cds", "sequence": "contig_1", "start": 276166, "stop": 277794, "strand": "+", "frame": 1, "gene": null, "product": "class 1b ribonucleoside-diphosphate reductase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AFF10F" ], "nt": "ATGACATCCAAAGAAAAAATCCCTACTTTTAATACTGAAGAAGATGTTGAAAGTTACATTTCTTTTAATGCCCAAGCCAAAATCTATGATGATTTTGCAATCGATTTACAAGCAGTTGAAAGCTATATTCAAGAGCATGTAAAACCCAAAACCAAGGTCTTTCATTCCACCAAAGAACGCCTTGATTTTCTGATTAAGAACGATTATTATGATGAGAAGATCATCAACATGTACAGTTTTGAACAGTTTGAAGAGATCACCCATAAAGCATATTCATACCGCTTTCGTTATGCTAACTTCATGGGAGCATTTAAGTTCTATAATGCCTATGCTTTAAAGACATTTGATGGTAAGTACTACTTGGAAAACTATGAGGATAGGGTGGTGATGAATGTATTGATGTTAGCTAATGGCAACTTCAATAAGGCATTAAAACTCTTAAAACAGATTATCCTTAACCGTTTTCAACCAGCAACCCCTACCTTTCTTAATGCTGGTAGAAAGAAACGTGGTGAATTTGTTTCATGTTACCTGTTAAGGATTGAAGATAACATGGAATCAATAGGTAGAGCGATAACAACTACACTACAACTATCAAAACGTGATGGGGGAGTAGCACTTTTGCTTTCCAACTTACGTGAAGCGGGAGCGCCCATCAAAAAGATAGAAAACCAATCATCAGGGATTATCCCAATTATGAAATTGTTAGAGGACTCTTTTTCCTATTCCAACCAACTTGGACAAAGACAAGGAGCGGGAGCGGTGTATCTCCATTGTCACCATCCTGATGTTATGCAGTTTTTAGATACTAAAAGGGAAAATGCTGATGAGAAGATCAGAATTAAATCACTCTCCTTAGGACTTGTGATTCCAGATATCACCTTCCAATTAGCAAAAAATAACGAGATGATGGCACTTTTCAGTCCATATGATATCTATCAGGAGTATGGTAAGGCTTTATCTGATATCTCAGTAACTGAGATGTATTATGAATTGCTTGAAAACCAACGCATTAAAAAGACCTTTATTAGTGCTAGAAAGTTCTTTCAAACAATTGCTGAACTCCACTTTGAAAGTGGTTATCCCTACATCTTGTTTGATGATACAGTTAACAGGAGAAATGCCCACAAAAACAGGATAGTAATGTCTAACCTTTGCAGTGAAATTGTCCAACCATCTTTACCTTCTGAATTCTATTCAGACCTTACTTTTAAAAAGGTAGGTAGTGATATTAGCTGTAACTTGGGGAGTTTAAATATTGCTAGAGCAATGGAAAGTGGTAGTGAGTTAGCTGAATTGATTCAACTAGCAATTGAATCACTGGATTTAGTGTCAAGGATCAGTAGTTTAGAAACCGCTCCTTCCATTAAAAAAGGTAATTCAGAAAACCATGCGTTGGGATTAGGAGCGATGAACTTACATGGATTTTTAGCAACAAATGCTATCTATTATGATTCAAAGGAAGCGGTTGATTTTACTAACATCTTTTTTTATACAGTAGCATACCATGCGTTTAGTGCTTCCAATAAATTAGCATTGGAACTAGGTAAATTTAAAGACTTTGAAAATACTAAATTTGCTGATGGTAGTTACTTTGATAAGTACACTAAGGTAGCTAGTGACTTTTGA", "aa": "MTSKEKIPTFNTEEDVESYISFNAQAKIYDDFAIDLQAVESYIQEHVKPKTKVFHSTKERLDFLIKNDYYDEKIINMYSFEQFEEITHKAYSYRFRYANFMGAFKFYNAYALKTFDGKYYLENYEDRVVMNVLMLANGNFNKALKLLKQIILNRFQPATPTFLNAGRKKRGEFVSCYLLRIEDNMESIGRAITTTLQLSKRDGGVALLLSNLREAGAPIKKIENQSSGIIPIMKLLEDSFSYSNQLGQRQGAGAVYLHCHHPDVMQFLDTKRENADEKIRIKSLSLGLVIPDITFQLAKNNEMMALFSPYDIYQEYGKALSDISVTEMYYELLENQRIKKTFISARKFFQTIAELHFESGYPYILFDDTVNRRNAHKNRIVMSNLCSEIVQPSLPSEFYSDLTFKKVGSDISCNLGSLNIARAMESGSELAELIQLAIESLDLVSRISSLETAPSIKKGNSENHALGLGAMNLHGFLATNAIYYDSKEAVDFTNIFFYTVAYHAFSASNKLALELGKFKDFENTKFADGSYFDKYTKVASDF", "aa_hexdigest": "631bb5a82b2d3b009845328d30d3072b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001C049A88", "query_cov": 0.9022140221402214, "subject_cov": 0.9683168316831683, "identity": 0.6890000000000001, "score": 687.0, "evalue": 8.28e-244, "valid": false, "product": "ribonucleoside-diphosphate reductase subunit alpha", "ec_ids": [ "1.17.4.1" ], "uniref50_id": "UniRef50_UPI0021AFF10F", "go_ids": [ "GO:0004748", "GO:0005971", "GO:0009265" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0021AFF10F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AFF10F" ], "product": "class 1b ribonucleoside-diphosphate reductase subunit alpha" }, "genes": [], "id": "KMADJIBOEG_533", "locus": "GCA000292445_00507" }, { "type": "cds", "sequence": "contig_1", "start": 277873, "stop": 278331, "strand": "+", "frame": 1, "gene": null, "product": "Ribonucleotide-diphosphate reductase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A655JDN6" ], "nt": "TTGGTAGCAAGTATCCAAAAAGATGGACTTGCAAACTCCCATTTAATGGCTATTGCCCCAACTGGATCTATCTCATATCTCTCTTCATGTACCCCTTCACTTCAACCAGTAGTATCTCCTGTTGAAGTGAGAAAAGAAGGGAAGTTAGGACGGATTTATGTCCCTGCTTATAAGCTTGATAATGATAACTATCAGTACTTTAAAGATGGTGCTTATGAACTGGGCTTTGAACCTATTATTAACATAGTAGCAGCAGCCCAACAACATGTTGATCAAGCAATCTCTTTAACCTTGTTTATGACTGATAAAGCTACCACCAGAGATCTCAATAAAGCTTATATTTATGCTTTTAAAAAGGGTTGTAGTTCTATCTATTATGTCAGAGTAAGACAAGATGTTTTAAAAGATAGTGAAGATCACACTATTAAAATCAAGGATTGTGAGGTTTGTTCTATCTAA", "aa": "MVASIQKDGLANSHLMAIAPTGSISYLSSCTPSLQPVVSPVEVRKEGKLGRIYVPAYKLDNDNYQYFKDGAYELGFEPIINIVAAAQQHVDQAISLTLFMTDKATTRDLNKAYIYAFKKGCSSIYYVRVRQDVLKDSEDHTIKIKDCEVCSI", "aa_hexdigest": "ed87e14e2ff7b0324ee5d6ca5ad0f03d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A655JDN6", "query_cov": 0.9736842105263158, "subject_cov": 1.0422535211267605, "identity": 0.52, "score": 157.0, "evalue": 6.68e-47, "valid": false, "product": "Ribonucleotide-diphosphate reductase subunit alpha", "uniref50_id": "UniRef50_A0A655JDN6" }, "pscc": { "uniref50_id": "UniRef50_A0A655JDN6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A655JDN6" ], "product": "Ribonucleotide-diphosphate reductase subunit alpha" }, "genes": [], "id": "KMADJIBOEG_534", "locus": "GCA000292445_00508" }, { "type": "cds", "sequence": "contig_1", "start": 278424, "stop": 278726, "strand": "+", "frame": 3, "gene": "rplU", "product": "50S ribosomal protein L21", "db_xrefs": [ "COG:COG0261", "COG:J", "GO:0003723", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02888", "RefSeq:WP_009885769.1", "SO:0001217", "UniParc:UPI0000133D46", "UniRef:UniRef100_P47474", "UniRef:UniRef50_P47474", "UniRef:UniRef90_P47474" ], "nt": "ATGCATGCTATTGTGGTTTGTGGTGCTAAGCAGTATTTAGTCCATGAAAACGAGTCTATTTTTGTTGAAAAATTAGCTGGTAAAGTTGGTCAGGAGATCCAACTTGATAAGGTATTGATGCTTGATGAAAAGATAGGCAAACCTTACCTTGAAAAAGCTAAGGTTGTTTGTGTGATTGAAAAACACGGTTTAAAATCGAAAATTAAACTAATTAAACACATCTCCCAAAAACACCACCTCAAGCGTTATGGCCACCGTCAACCCTACACCAAACTAAAAGTGGTACGCTTTATCCATGATTAA", "aa": "MHAIVVCGAKQYLVHENESIFVEKLAGKVGQEIQLDKVLMLDEKIGKPYLEKAKVVCVIEKHGLKSKIKLIKHISQKHHLKRYGHRQPYTKLKVVRFIHD", "aa_hexdigest": "3e7656c95e5048e12de9eb4e3e4b4a27", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133D46", "ncbi_nrp_id": "WP_009885769.1", "uniref100_id": "UniRef100_P47474", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133D46", "RefSeq:WP_009885769.1", "UniRef:UniRef100_P47474" ] }, "ips": { "uniref100_id": "UniRef100_P47474", "uniref90_id": "UniRef90_P47474" }, "psc": { "uniref90_id": "UniRef90_P47474", "gene": "rplU", "product": "50S ribosomal protein L21", "uniref50_id": "UniRef50_P47474", "cog_id": "COG0261", "cog_category": "J", "kegg_orthology_id": "K02888", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P47474", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47474" ], "product": "Large ribosomal subunit protein bL21" }, "genes": [ "rplU" ], "id": "KMADJIBOEG_535", "locus": "GCA000292445_00509" }, { "type": "cds", "sequence": "contig_1", "start": 278719, "stop": 279018, "strand": "+", "frame": 1, "gene": "prp", "product": "Ribosomal processing cysteine protease Prp", "db_xrefs": [ "COG:COG2868", "COG:S", "EC:3.4.22.-", "GO:0006508", "GO:0008234", "GO:0042254", "KEGG:K07584", "RefSeq:WP_009885770.1", "SO:0001217", "UniParc:UPI00001394CA", "UniRef:UniRef100_P47475", "UniRef:UniRef50_P47475", "UniRef:UniRef90_P47475" ], "nt": "ATGATTAAGATTAATATCTCCCAAAACTTTCTAGTTGCAAAAGGTCATGCTTTGTTTGCTGAGAAGGGTAAGGACATAGTTTGTGCTGCAATTAGTGGAATTATCTTTGGGGGGGTGGCTTGGTTTGAACCTGATAAGATTGAATTTACTGAAAATAAATTAGTACCTAGTATAGCACTGAAACTCATTGACCCAACCCCTAATGTAGCAGTTGCTTTTAGTGTTATTACAGTACAATTAAAAGCAATAGCCAATTCCTATCCTAATCACATAGTTATCAATGAAGAGAGTTATGAGTAA", "aa": "MIKINISQNFLVAKGHALFAEKGKDIVCAAISGIIFGGVAWFEPDKIEFTENKLVPSIALKLIDPTPNVAVAFSVITVQLKAIANSYPNHIVINEESYE", "aa_hexdigest": "6ec548ab6a67574f06e1d557cff348cd", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394CA", "ncbi_nrp_id": "WP_009885770.1", "uniref100_id": "UniRef100_P47475", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394CA", "RefSeq:WP_009885770.1", "UniRef:UniRef100_P47475" ] }, "ips": { "uniref100_id": "UniRef100_P47475", "uniref90_id": "UniRef90_P47475" }, "psc": { "uniref90_id": "UniRef90_P47475", "gene": "prp", "product": "Ribosomal processing cysteine protease Prp", "ec_ids": [ "3.4.22.-" ], "uniref50_id": "UniRef50_P47475", "cog_id": "COG2868", "cog_category": "S", "kegg_orthology_id": "K07584", "go_ids": [ "GO:0008234", "GO:0006508", "GO:0042254" ] }, "pscc": { "uniref50_id": "UniRef50_P47475", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47475" ], "product": "Ribosomal processing cysteine protease Prp" }, "genes": [ "prp" ], "id": "KMADJIBOEG_536", "locus": "GCA000292445_00510" }, { "type": "cds", "sequence": "contig_1", "start": 279011, "stop": 279325, "strand": "+", "frame": 2, "gene": "rpmA", "product": "50S ribosomal protein L27", "db_xrefs": [ "COG:COG0211", "COG:J", "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02899", "RefSeq:WP_014894232.1", "SO:0001217", "UniParc:UPI00027B3BE5", "UniRef:UniRef100_UPI00027B3BE5", "UniRef:UniRef50_P05657", "UniRef:UniRef90_P47476" ], "nt": "ATGAGTAAAAAAAGTTACTGCTACCAAATTAACTTACAGTTTTTCGCTTCCAAAAAAGGGGTTGGTTCCACTAAGAATGGACGTGATTCACATTCTAAGCGCTTGGGCGCTAAGAAGGCAGATGGTCAGATGATTAGAGTTGGTCAGATTATCTATAGACAAAGGGGAACTAAGATCTTTGCAGGACAAAATGTTGCAATGGGTAGTGATAACACCCTCTTTGCTTTGAGCGATGGCATTGTCCGCTTTCAAAAGTTTGGTAGTAAACAAAGCAAAACCCGGGTTAACATCATCAAACACCAACTAAATGCCTAA", "aa": "MSKKSYCYQINLQFFASKKGVGSTKNGRDSHSKRLGAKKADGQMIRVGQIIYRQRGTKIFAGQNVAMGSDNTLFALSDGIVRFQKFGSKQSKTRVNIIKHQLNA", "aa_hexdigest": "99b0114b5eb4292df0dcaf87468cba3b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3BE5", "ncbi_nrp_id": "WP_014894232.1", "uniref100_id": "UniRef100_UPI00027B3BE5", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3BE5", "RefSeq:WP_014894232.1", "UniRef:UniRef100_UPI00027B3BE5" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3BE5", "uniref90_id": "UniRef90_P47476" }, "psc": { "uniref90_id": "UniRef90_P47476", "gene": "rpmA", "product": "50S ribosomal protein L27", "uniref50_id": "UniRef50_P05657", "cog_id": "COG0211", "cog_category": "J", "kegg_orthology_id": "K02899", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P05657", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P05657" ], "product": "Large ribosomal subunit protein bL27" }, "genes": [ "rpmA" ], "id": "KMADJIBOEG_537", "locus": "GCA000292445_00511" }, { "type": "cds", "sequence": "contig_1", "start": 279318, "stop": 280163, "strand": "+", "frame": 3, "gene": "nfo", "product": "putative endonuclease 4", "db_xrefs": [ "COG:COG0648", "COG:L", "EC:3.1.21.2", "GO:0003677", "GO:0003906", "GO:0006284", "GO:0008081", "GO:0008270", "GO:0008833", "SO:0001217", "UniRef:UniRef50_P47477", "UniRef:UniRef90_P47477" ], "nt": "ATGCCTAAGCTACTGGGTAGCTTTATTAGCTTTAAAGCCCCCAATTACTTTGTTCAAAGTGCTCAGGATGCAATTGCTATTGATGCAACTGCTCTAATGGTATTTTTAGGTCCACCCCATTCTGCTTACCGTGTTCCTTTTAACAAGATGCAGTTTAGTTTGGGCTATGAGTTGTTAAAAACAAAGAATATTAATAGTAATGGTTTGGTTGTTCATGCTCCATATATCATTAACTGTGCATCAAAAGACCCACTAAAACAGCAGAATGCTATCAGTGTTTTAACCAATGAGATTCAGCTTTGTAACTTGGCTGGTGCTCACTATTTAGTTTTGCATCCAGGTTCTGCAGTAGCCCAAACAACCAACGAAGCATTAGATAACCTGGTTAAAGTACTCAATCAAGTTATCAATAAAACCAAAACAACAGTTATTTGCCTTGAAACAATGGCTGGTAAGGGTAATGAGATAGGCAGAGACTTAACTGAGTTGAAATATGTTATTGACAGGATCGTTGATAAAGATAGGATCGGGGTTTGTCTTGATACCTGTCACTTCCATGATAGTGGGATTGACTTTAGTGATCTAACTGGTGTTTTTAACACCATTACAACTAAACTTGGTTTTGAGTTTCTCAAAGTAATCCATTTAAATGAATCTAAAAATAATTGTGGTTCTAAAAAAGATAGGCATGCTAATATCAATGCTGGGATGATTGGTTTTGAGAACTTAATGAAGTTCATTAGTCACCCCCAAATTAAGGATTTACCTATTATCTTAGAAACCCCTTCAACTAGTTTAAACTACCCAACTATTTACCGTGAAGAGATTAGTCAAATCCGCAGCTGA", "aa": "MPKLLGSFISFKAPNYFVQSAQDAIAIDATALMVFLGPPHSAYRVPFNKMQFSLGYELLKTKNINSNGLVVHAPYIINCASKDPLKQQNAISVLTNEIQLCNLAGAHYLVLHPGSAVAQTTNEALDNLVKVLNQVINKTKTTVICLETMAGKGNEIGRDLTELKYVIDRIVDKDRIGVCLDTCHFHDSGIDFSDLTGVFNTITTKLGFEFLKVIHLNESKNNCGSKKDRHANINAGMIGFENLMKFISHPQIKDLPIILETPSTSLNYPTIYREEISQIRS", "aa_hexdigest": "1ff46dd14e955ac826d0954a7552843b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47477", "query_cov": 1.0, "subject_cov": 0.9656357388316151, "identity": 1.0, "score": 560.0, "evalue": 1.4e-201, "valid": true, "gene": "nfo", "product": "Probable endonuclease 4", "ec_ids": [ "3.1.21.2" ], "uniref50_id": "UniRef50_P47477", "cog_id": "COG0648", "cog_category": "L", "go_ids": [ "GO:0008833", "GO:0003677", "GO:0003906", "GO:0008081", "GO:0008270", "GO:0006284" ] }, "pscc": { "uniref50_id": "UniRef50_P47477", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47477" ], "product": "Probable endonuclease 4" }, "genes": [ "nfo" ], "id": "KMADJIBOEG_538", "locus": "GCA000292445_00512" }, { "type": "cds", "sequence": "contig_1", "start": 280183, "stop": 280605, "strand": "+", "frame": 1, "gene": "fur", "product": "putative protein MG236", "db_xrefs": [ "COG:COG0735", "COG:P", "GO:0000976", "GO:0003700", "GO:0008270", "GO:0045892", "GO:1900376", "RefSeq:WP_014894233.1", "SO:0001217", "UniParc:UPI00027B3BE6", "UniRef:UniRef100_UPI00027B3BE6", "UniRef:UniRef50_P75456", "UniRef:UniRef90_P47478" ], "nt": "ATGCTAACTAGTTATGCGAAGGTATTAGAACAAAACAATCTGCGCTTAACAAAACCGCGGATTGCTTTATTAAAGTGTTTAATTGAACACCAAGATTGGCATAATCTCTCCCAAATTAAAACCCACCTTGATTTAGCCAATCAACCCTCAACACTCGCTTCTCTCTACAACAACTTAAGAATCCTAGCTAAACTTAAACTGATTAACATCTTTGTAGATCCAGAGCGGTTTGAAACTTACTATTGCCTGCGCCATGCAGAGCATAACCATATCTATCTTTTTGATGAAGTTAAACAGCAGTTTTTTACCTTACCTTTAACAGATGGCCAAATTAAAACATTGCTTGAAACCCAAAACCATACCAGTAAGGTAAAGCTTAATGATTTTTATATTGTTGCTAGGGGTGAGATAAACAATGATTAA", "aa": "MLTSYAKVLEQNNLRLTKPRIALLKCLIEHQDWHNLSQIKTHLDLANQPSTLASLYNNLRILAKLKLINIFVDPERFETYYCLRHAEHNHIYLFDEVKQQFFTLPLTDGQIKTLLETQNHTSKVKLNDFYIVARGEINND", "aa_hexdigest": "cf0602f4b2d9f897be48984f4f218e19", "start_type": "ATG", "rbs_motif": "ATTTA", "ups": { "uniparc_id": "UPI00027B3BE6", "ncbi_nrp_id": "WP_014894233.1", "uniref100_id": "UniRef100_UPI00027B3BE6", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3BE6", "RefSeq:WP_014894233.1", "UniRef:UniRef100_UPI00027B3BE6" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3BE6", "uniref90_id": "UniRef90_P47478" }, "psc": { "uniref90_id": "UniRef90_P47478", "gene": "Fur", "product": "Uncharacterized protein MG236", "uniref50_id": "UniRef50_P75456", "cog_id": "COG0735", "cog_category": "P", "go_ids": [ "GO:0003700", "GO:0000976", "GO:0008270", "GO:0045892", "GO:1900376" ] }, "pscc": { "uniref50_id": "UniRef50_P75456", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75456" ], "product": "Uncharacterized protein MG236 homolog" }, "genes": [ "fur" ], "id": "KMADJIBOEG_539", "locus": "GCA000292445_00513" }, { "type": "cds", "sequence": "contig_1", "start": 280598, "stop": 281482, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MG237", "db_xrefs": [ "RefSeq:WP_009885774.1", "SO:0001217", "UniParc:UPI00001394D3", "UniRef:UniRef100_P47479", "UniRef:UniRef50_P75455", "UniRef:UniRef90_P47479" ], "nt": "ATGATTAACAAACCAAACCAATTTTTAAACCACCTTGATGGCTTAAAGCAACATTTTTCTGATTATGATTCACTACAAAAATCCTTTAAGAAATATCTATCAGAAAATCAAACTGAACTTAACAATTTCTTTTTTAACCAGTTTGAAAAGATCATTGTTCTGGTCAAAAAAAAGGAGTTTAAAACTGCTCAAGAGAGGTGTGAAGAGGAGTTAGCTACCCCTTATTTTTCAAAGCCATTGGTTGGTTTTTTCCAATCACTATTACAACTAATTAACCATGATCTCATCGAACAGAAAAACCAACAGTTAGCTAACATGAGTTGTGAAAAAATTGTGGAGATGGTGTTAAGTGATTACCCCAATAAACTTAACTTAATCCACTATTTGTTAGCAAAAGAAGCGAGTTTTGTAAACCCTAACCTTTTACAGCGGATGACTTTTGTGTTAACTGATCTTGAACTGTTAGAGTTAAAGCGTTTCTCTTTTTTTAAAGCCCTTAACCAGATACCTGCTTTTAAAAACCACAAAGTAACATACTTTAACAGCAAACTCAAACAGAAGTTTGTAATAACATTAGGTGAATTTGCTTTCCCCCAAACTGATAAAACCAAACAGTTTTTCCAACAACTAATTAAAAAAGTAAGTCAACTGTTTTTAAAAGAACCTGTTAGTTGTGAATTTGCTTATGAAATTATTGATGCATTACTCGTCAGTTTTTTTCCACTCCATCCTAATTTAGAAGTAAACCACTTAGCTAAAAAGATCCACCAGTATGTTAGTAAGATTGTCATTAATGAAGTTGTTGATCTGAAAGATCCAACCACTAAACTAATTGTTGATACACTTTATGAACAGTTAGATAGAGCAATTGGTGAGGAAAATTAA", "aa": "MINKPNQFLNHLDGLKQHFSDYDSLQKSFKKYLSENQTELNNFFFNQFEKIIVLVKKKEFKTAQERCEEELATPYFSKPLVGFFQSLLQLINHDLIEQKNQQLANMSCEKIVEMVLSDYPNKLNLIHYLLAKEASFVNPNLLQRMTFVLTDLELLELKRFSFFKALNQIPAFKNHKVTYFNSKLKQKFVITLGEFAFPQTDKTKQFFQQLIKKVSQLFLKEPVSCEFAYEIIDALLVSFFPLHPNLEVNHLAKKIHQYVSKIVINEVVDLKDPTTKLIVDTLYEQLDRAIGEEN", "aa_hexdigest": "3ddd0b764b47994e1c3f34780f7355db", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394D3", "ncbi_nrp_id": "WP_009885774.1", "uniref100_id": "UniRef100_P47479", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394D3", "RefSeq:WP_009885774.1", "UniRef:UniRef100_P47479" ] }, "ips": { "uniref100_id": "UniRef100_P47479", "uniref90_id": "UniRef90_P47479" }, "psc": { "uniref90_id": "UniRef90_P47479", "product": "Uncharacterized protein MG237", "uniref50_id": "UniRef50_P75455" }, "pscc": { "uniref50_id": "UniRef50_P75455", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75455" ], "product": "Uncharacterized protein MG237 homolog" }, "genes": [], "id": "KMADJIBOEG_540", "locus": "GCA000292445_00514" }, { "type": "cds", "sequence": "contig_1", "start": 281623, "stop": 282846, "strand": "+", "frame": 1, "gene": "tig", "product": "trigger factor", "db_xrefs": [ "COG:COG0544", "COG:O", "EC:5.2.1.8", "GO:0003755", "GO:0005737", "GO:0005854", "GO:0015031", "GO:0043022", "GO:0043335", "GO:0044183", "GO:0051082", "GO:0051083", "GO:0051301", "GO:0061077", "GO:0140839", "GO:0140840", "SO:0001217", "UniRef:UniRef50_P47480", "UniRef:UniRef90_P47480" ], "nt": "ATGGCTAAATCGATAAAAATTAAGGGTTTTCGTCCCGGTAAAATCCCCCCTAATTTAGCCAGTCAGTCGATTAATAAAGCTGAATTAATGCAAAAAAGTGCCCAAAACGTCATGAACAGTATTTATGAATCAGTTCAACAAGAAGAGATCGTTGCTAGTAATGATAATGTCATTGATGATTATCCTACCATTGATTTCAAAACGATCACTGAACAAAACTGTGTACTTTTGTTTTACTTTGATCTGATCCCTAACTTTCAACTCCCTGATTACAAAAAGATAAAAGATTTAACACCACTTACCAAGTTAACTGAAGCTGAATTTAACAACGAAATTGAAAAGCTGGCAAAAACTAAAAGCACAATGGTAGATGTTAGTGATAAAAAACTAGCTAATGGTGATATTGCTATCATTGATTTCACTGGGATAGTTGATAACAAAAAACTAGCATCAGCTTCAGCACAAAACTATGAATTGACAATTGGTTCAAATAGCTTTATTAAGGGTTTTGAAACCGGGTTAATAGCAATGAAAGTTAACCAGAAAAAAACTTTAGCACTAACTTTTCCTAGTGATTATCATGTTAAGGAGTTGCAATCAAAACCAGTTACATTTGAAGTAGTTTTAAAAGCAATTAAAAAACTGGAATTCACCCCAATGGATGAAACTAATTTCAAATCCTTTCTCCCTGAACAATTCCAAAGCTTTACTTCTCTAAAGGCATTTAAGAGTTATTTTCATAAGCTAATGGAAAACAAAAAACAAGAGACAATTCTCCAGGAGAATAACCAAAAAATTCGTCAGTTCTTACTTACTAATACCAAACTTCCTTTTCTTCCAGAAGCGTTAATTAAACTAGAAGCTAACCGCTTGTTAAAGCTCCAGCAAAGCCAAGCTGAACAATATAAAATCCCCTTTGAAAAACTCTTAAGTGCTTCTAATATCACCCTAACAGAGTTACAAGATCGCAACATAAAAGAAGCTAAGGAAAATGTTACCTTTGCTTTGGTAATGAAAAAGATAGCTGACATTGAAAAGATTAAGGTTGATAATAACAAGATTAAAGCTGAAATTGAAAATGTTATTGCTGTTGAATATCCCTTTGCTAGTGATGAAATGAAAAAACAACTCTTTTTTAATATGGAACAACAAAAGGAGTTTGTGGAATCAATTATCATCAACAGATTAACAACAACTAAAATCGTTAGCTATTCAACTCATTAG", "aa": "MAKSIKIKGFRPGKIPPNLASQSINKAELMQKSAQNVMNSIYESVQQEEIVASNDNVIDDYPTIDFKTITEQNCVLLFYFDLIPNFQLPDYKKIKDLTPLTKLTEAEFNNEIEKLAKTKSTMVDVSDKKLANGDIAIIDFTGIVDNKKLASASAQNYELTIGSNSFIKGFETGLIAMKVNQKKTLALTFPSDYHVKELQSKPVTFEVVLKAIKKLEFTPMDETNFKSFLPEQFQSFTSLKAFKSYFHKLMENKKQETILQENNQKIRQFLLTNTKLPFLPEALIKLEANRLLKLQQSQAEQYKIPFEKLLSASNITLTELQDRNIKEAKENVTFALVMKKIADIEKIKVDNNKIKAEIENVIAVEYPFASDEMKKQLFFNMEQQKEFVESIIINRLTTTKIVSYSTH", "aa_hexdigest": "4f0adacbb74f6492382dc9af35ead775", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47480", "query_cov": 1.0, "subject_cov": 0.9166666666666666, "identity": 1.0, "score": 763.0, "evalue": 3.29e-277, "valid": true, "gene": "tig", "product": "trigger factor", "ec_ids": [ "5.2.1.8" ], "uniref50_id": "UniRef50_P47480", "cog_id": "COG0544", "cog_category": "O", "go_ids": [ "GO:0003755", "GO:0005737", "GO:0005854", "GO:0015031", "GO:0043022", "GO:0043335", "GO:0044183", "GO:0051082", "GO:0051083", "GO:0051301", "GO:0061077", "GO:0140839", "GO:0140840" ] }, "pscc": { "uniref50_id": "UniRef50_P47480", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47480" ], "product": "Trigger factor" }, "genes": [ "tig" ], "id": "KMADJIBOEG_541", "locus": "GCA000292445_00515" }, { "type": "cds", "sequence": "contig_1", "start": 282915, "stop": 283892, "strand": "+", "frame": 3, "gene": null, "product": "LON peptidase substrate-binding domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F21836E" ], "nt": "ATGCCCGTTACGAAGAAAAGTCAGATCTTAGTAGTTAGAGGTCAAGTCATTTTTCCTTTTGTTCCCTTTAGTTTGGATGTTGGCAGGCCCCGTTCGCGTAAGATCATCAAAGCGCTTAAAACTCTGAAAACCAAACGTTTGGTTTTAGTAACCCAAAAGTTTACTGGTGAACAAAACCCTGAGTTTAATGACATCTATCATGTCGGTACACTCTGTGAGATTGATGAGATAGTTGATGTTCCAGGGGTTGATAGTAAAACAGTAGACTACCGTATTAAAGGCAGAGGTTTACAACGGGTTTTAATTGAAAAATTCTCAGATGCAGATATTAATGAAGTTAGTTACCAATTACTTAACTCCACAGTTAAAGATGAAGCTAATGTTGACAGGTTCTTACAGCGAATCTTTCCTGAAAAAGAAGAAATTGAACAGTTAATGGAAGGAGCTGAGAAGTTTTTAGAACTTGAAAACATCAGCAAAACAGTTAATGTTCCTAAGGGTTTAAAGCAACTTGATATTATCACCTTTAAACTGGCTAATCTTGTCCCTAACACTGAAAGTATTAAACAAGCTATCTTAGAGGAAAATGAGATAGCAAACCGATTGGAAAAGATTATCCAAGCAGGGATTGAAGATTTACAGAAGATCCAAGATTATGGTAGATCTAAAAACAAGGAAACTGAGTTTGATAAACTTGACAGTAAAATTACCCGCAAAATTAACGAACAACTCTCAAGACAACAACGTGATTTCTATCTTCGTGAAAAGCTAAGAATTATCCGTGAAGAGATAGGGATTAGTTCCAAAAAAGAGGATGAAGTTGCTAGTATTAGAAAGAAACTGGATGAAAACCCTTACCCTGAAGCCATTAAAAAACGGATTTTAAGTGAACTTGAACACTATGAAAACTCTTCCTCCTCTTCCCAAGAATCAACCTTAACCAAAACTTACATTGATACGCTTTTAAACCTGCCTTGA", "aa": "MPVTKKSQILVVRGQVIFPFVPFSLDVGRPRSRKIIKALKTLKTKRLVLVTQKFTGEQNPEFNDIYHVGTLCEIDEIVDVPGVDSKTVDYRIKGRGLQRVLIEKFSDADINEVSYQLLNSTVKDEANVDRFLQRIFPEKEEIEQLMEGAEKFLELENISKTVNVPKGLKQLDIITFKLANLVPNTESIKQAILEENEIANRLEKIIQAGIEDLQKIQDYGRSKNKETEFDKLDSKITRKINEQLSRQQRDFYLREKLRIIREEIGISSKKEDEVASIRKKLDENPYPEAIKKRILSELEHYENSSSSSQESTLTKTYIDTLLNLP", "aa_hexdigest": "055cd860e4b9ddb0b1d959506f0a4f0f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F21836E", "query_cov": 0.803076923076923, "subject_cov": 1.0, "identity": 0.594, "score": 303.0, "evalue": 1.14e-99, "valid": false, "product": "LON peptidase substrate-binding domain-containing protein", "uniref50_id": "UniRef50_UPI001F21836E" }, "pscc": { "uniref50_id": "UniRef50_UPI001F21836E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F21836E" ], "product": "LON peptidase substrate-binding domain-containing protein" }, "genes": [], "id": "KMADJIBOEG_542", "locus": "GCA000292445_00516" }, { "type": "cds", "sequence": "contig_1", "start": 284037, "stop": 285302, "strand": "+", "frame": 3, "gene": "lon", "product": "endopeptidase La", "db_xrefs": [ "COG:COG0466", "COG:O", "EC:3.4.21.53", "GO:0004176", "GO:0004252", "GO:0005524", "GO:0005737", "GO:0006508", "GO:0006515", "GO:0016887", "GO:0034605", "GO:0043565", "SO:0001217", "UniRef:UniRef50_P47481", "UniRef:UniRef90_P47481" ], "nt": "ATGTGTTTAGTAGGTCCTCCTGGGGTTGGTAAATCAAGTCTAGCTAAGTCTATTGCAGAAGCATTAGATAAGAAGTTTGTCAAGATCTCATTAGGGGGAGTACATGATGAATCGGAAATCAGAGGTCACCGTAAAACTTACTTAGGTTCTATGCCAGGAAGGATTTTGAAAGGGATGATCCGTGCTAAGGTAATTAATCCCCTCTTTTTACTTGATGAAATTGATAAGATGACCTCCTCCAACCAAGGTTATCCTTCAGGTGCTTTACTTGAAGTATTAGATCCAGAGTTAAATAATAAGTTTAGTGATAACTATGTTGAAGAAGATTATGATCTTTCTAAAGTAATGTTTATCGCTACTGCAAACTACATAGAAGATATCCCTGAAGCTTTACTTGATAGGATGGAGATAATTGAACTCACTTCCTATACAGAACAAGAGAAGATTGAGATAGCAAAAAACCACTTAATTAAGCGTTGCTTTGAGGATGCTGATCTTAACAGTGAAGAATTGAAGTTCACTGATGAAGCAATCAGCTACATCATTAAGTTTTACACAAGAGAAGCGGGGGTTAGACAATTAGAACGATTAATCCAACAAGTTGTAAGAAAGTACATAGTAGCAATGCAAAAAGATGGCATCAAACAAGAAACGATTGATGTAAACGCTGTTAAAAAATACCTTAAGAAGGAGATCTTTGATCACACTATGCGTGATGAAGTGTCTCTACCTGGAATTGTCAACGGGATGGCATACACCCCAACTGGAGGGGACTTACTTCCCATAGAAGTTACCCATGTTGCTGGTAAAGGAGAGTTGATCTTAACTGGTAATTTAAAGCAAACAATGCGAGAAAGCGCTAATGTTGCTTTAGGCTATGTAAAAGCTAATGCAGAGCGTTTTAACATTAATCCTAGTTTGTTTAAAAAGATTGATATTAACATCCATGTTCCAGGTGGGGGAATTCCTAAGGATGGACCTAGTGCTGGTGCTGCTTTGGTAACTGCAATCATCTCATCATTAACTGGTAAGAAAGTAGATCCTACAGTGGCTATGACAGGAGAGATCACTTTAAGAGGCAAAGTGTTGGTTATTGGTGGGGTGAAAGAAAAAACTATCTCAGCTTACCGCGGTGGGGTTACAACTATCTTTATGCCTGAGAAAAACGAGCGCTATTTAGATGAAGTACCCAAAGAGATAGTAGATAAACTTAACATTATCTTTGTTAAGGAATACAGTGATATCTACAACAAGCTTTTCAGTTAG", "aa": "MCLVGPPGVGKSSLAKSIAEALDKKFVKISLGGVHDESEIRGHRKTYLGSMPGRILKGMIRAKVINPLFLLDEIDKMTSSNQGYPSGALLEVLDPELNNKFSDNYVEEDYDLSKVMFIATANYIEDIPEALLDRMEIIELTSYTEQEKIEIAKNHLIKRCFEDADLNSEELKFTDEAISYIIKFYTREAGVRQLERLIQQVVRKYIVAMQKDGIKQETIDVNAVKKYLKKEIFDHTMRDEVSLPGIVNGMAYTPTGGDLLPIEVTHVAGKGELILTGNLKQTMRESANVALGYVKANAERFNINPSLFKKIDINIHVPGGGIPKDGPSAGAALVTAIISSLTGKKVDPTVAMTGEITLRGKVLVIGGVKEKTISAYRGGVTTIFMPEKNERYLDEVPKEIVDKLNIIFVKEYSDIYNKLFS", "aa_hexdigest": "50860e0ddcb6b4f1cb1342a1700630c2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0025901479", "query_cov": 0.9857482185273159, "subject_cov": 0.8169291338582677, "identity": 0.535, "score": 466.0, "evalue": 1.26e-158, "valid": false, "gene": "lon", "product": "endopeptidase La", "ec_ids": [ "3.4.21.53" ], "uniref50_id": "UniRef50_A0A7C6Y0Z4", "go_ids": [ "GO:0004252", "GO:0005524", "GO:0006508", "GO:0016887" ] }, "genes": [ "lon" ], "truncated": "5-prime", "pseudogene": { "start": 283737, "stop": 285302, "observations": { "mutation": [ 283890, 283893 ], "stop": [ 283890, 283893 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47481", "query_cov": 1.0, "subject_cov": 0.529559748427673, "identity": 0.995, "score": 815.0, "evalue": 3.88e-292, "gene_start": 1, "gene_end": 421, "reference_start": 375, "reference_end": 795, "reference_sequence": "MPVTKKSQILVVRGQVIFPFVPFSLDVGRPRSRKIIKALKTLKTKRLVLVTQKFTGEQNPEFNDIYHVGTLCEIDEIVDVPGVDSKTVDYRIKGRGLQRVLIEKFSDADINEVSYQLLNSTVKDEANVDRFLQRIFPEKEEIEQLMEGAEKFLELENISKTVNVPKGLKQLDIITFKLANLVPNTESIKQAILEENEIANRLEKIIQAGIEDLQKIQDYGRSKNKETEFDKLDSKITRKINEQLSRQQRDFYLREKLRIIREEIGISSKKEDEVASIRKKLDENPYPEAIKKRILSELEHYENSSSSSQESTLTKTYIDTLLNLPWWQKSKDNSDVKNLIKTLDKNHTGLDKVKERIVEYLAVQLRTQKNKGPIMCLVGPPGVGKSSLAKSIAEALDKKFVKISLGGVHDESEIRGHRKTYLGSMPGRILKGMTRAKVINPLFLLDEIDKMTSSNQGYPSGALLEVLDPELNNKFSDNYVEEDYDLSKVMFIATANYIEDIPEALLDRMEIIELTSYTEQEKIEIAKNHLIKRCLEDADLNSEELKFTDEAISYIIKFYTREAGVRQLERLIQQVVRKYIVAMQKDGIKQETIDVNAVKKYLKKEIFDHTMRDEVSLPGIVNGMAYTPTGGDLLPIEVTHVAGKGELILTGNLKQTMRESANVALGYVKANAERFNINPSLFKKIDINIHVPGGGIPKDGPSAGAALVTAIISSLTGKKVDPTVAMTGEITLRGKVLVIGGVKEKTISAYRGGVTTIFMPEKNERYLDEVPKEIVDKLNIIFVKEYSDIYNKLFS" }, "paralog": false, "identity": 0.9923224568138196, "score": 1005.0, "evalue": 0.0, "description": "Internal stop codon at 283890, 283893. Nonsense mutation around 283890, 283893.", "psc": { "uniref90_id": "UniRef90_P47481", "gene": "lon", "product": "endopeptidase La", "ec_ids": [ "3.4.21.53" ], "uniref50_id": "UniRef50_P47481", "cog_id": "COG0466", "cog_category": "O", "go_ids": [ "GO:0004176", "GO:0004252", "GO:0005524", "GO:0005737", "GO:0006508", "GO:0006515", "GO:0016887", "GO:0034605", "GO:0043565" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47481", "EC:3.4.21.53", "UniRef:UniRef50_P47481", "COG:COG0466", "COG:O", "GO:0004176", "GO:0004252", "GO:0005524", "GO:0005737", "GO:0006508", "GO:0006515", "GO:0016887", "GO:0034605", "GO:0043565" ] }, "pscc": { "uniref50_id": "UniRef50_P47481", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47481" ], "product": "Lon protease" } }, "id": "KMADJIBOEG_543", "locus": "GCA000292445_00517" }, { "type": "cds", "sequence": "contig_1", "start": 285299, "stop": 285517, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATTAATGCCATCAAAAACCACACCATCCCACCAAAACAAGTTAGCAAGCTAGATATGATTGTTTATTTAGCAGATAAACTAGAACCTAACCGTAAACAGGAACAGTGGAGTGGGGGAATTGAGATTGATCAACTAGTAAAACTAGCTAAAAGCAACTTAAAACAGGCCTATTTAATTACCTTAAAATATGTCCAAAACCTAGTTAAAGACTAA", "aa": "MIINAIKNHTIPPKQVSKLDMIVYLADKLEPNRKQEQWSGGIEIDQLVKLAKSNLKQAYLITLKYVQNLVKD", "aa_hexdigest": "ecff59f73e4a94e84c6848c30caa51b5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8290.6983, "isoelectric_point": 9.477255439758302 }, "id": "KMADJIBOEG_544", "locus": "GCA000292445_00518" }, { "type": "cds", "sequence": "contig_1", "start": 285581, "stop": 285982, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTGCACGTTTGTTTGTTATGAACGAAAACCTTATCCTTTTAATAAAAAAATAGCAAACCAATTTAATGTTAAATACTTGGCTAAATGCCCACTTGAAATCGCTTCAAGTAAACTTTTAAATCAACCTAGAAAAAAACTGATACCACTGGCAGTTTTAAATTACATCAATACCAATCACCTCTATCTAATTCCCACTTTAAAAGCAATGGTTGATGATAAGAGGTTTCAACACTGTTTAAGGGTTGGTAAACTAGCAAAACAACTAGCAATTGCAAACAAACTAGATGCTAAAAGAGCATTTGTTGCTGGGGCTTATCATGACTTGGCCAAACAACTACCAGTTGATCAGTTAGTTAACATTGCAACTAGTGAACTGAAGATAACTAACTACCCAAGTTGA", "aa": "MCTFVCYERKPYPFNKKIANQFNVKYLAKCPLEIASSKLLNQPRKKLIPLAVLNYINTNHLYLIPTLKAMVDDKRFQHCLRVGKLAKQLAIANKLDAKRAFVAGAYHDLAKQLPVDQLVNIATSELKITNYPS", "aa_hexdigest": "68f963d331e9133c1eeced9e4304f3cb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15161.832799999982, "isoelectric_point": 9.77219829559326 }, "id": "KMADJIBOEG_545", "locus": "GCA000292445_00519" }, { "type": "cds", "sequence": "contig_1", "start": 286007, "stop": 286351, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01467.32" ], "nt": "TTGAAGCAAAAAATTATTATCTTTGGTGGTTCTTTTGACCCTATTCACAATGCCCATCTCTACATTGCTAAACATGCAATTAAAAAAATAAAAGCGCAAAAGCTTTTTTTTGTACCAACTTACAATGGTATTTTTAAAAATAATTTTCATGCTAGTAATAAGGATAGGATTGCTATGTTAAAGCTAGCAATTAAATCAGTCAACAATGCTTTAGTTTCCAATTTTGATATTAAAACTAAAAACGCTTTTTCAATTAATACTGTTAACCACTTTAAAAGTTGTTATCCAACTAGTGAAATCTATTTTTTAATAGGGAGTGATAAATTAAATGAACTTGAGAAGTGA", "aa": "MKQKIIIFGGSFDPIHNAHLYIAKHAIKKIKAQKLFFVPTYNGIFKNNFHASNKDRIAMLKLAIKSVNNALVSNFDIKTKNAFSINTVNHFKSCYPTSEIYFLIGSDKLNELEK", "aa_hexdigest": "b29232cddfd57f3f64c02283d46472cf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01467.32", "name": "CTP_transf_like", "length": 106, "aa_cov": 0.9298245614035088, "hmm_cov": 0.8867924528301887, "evalue": 1.2510422229078527e-16, "score": 56.22679138183594, "start": 7, "stop": 112 } ], "seq_stats": { "molecular_weight": 13020.078299999983, "isoelectric_point": 9.834990501403805 }, "id": "KMADJIBOEG_546", "locus": "GCA000292445_00520" }, { "type": "cds", "sequence": "contig_1", "start": 286404, "stop": 286862, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCCAAAAGGAGATGGAGATCTATAACCTCTTTACTTTTCAAATTGATCTTGATAAAAAACTGTTGTTTGAAAAATCAAATGATCAGAAGAATTATTCCAAGATTAGAACCCATTATTTCAAGCATAAATTCAAAAATAAAAGTGCTGTTTTTCTCAATAAAAACCTGATTAAAAATAGCTTAAATAAGGTTCTTTTAAACTTTTCTGATTTTGTTAGTGGTGCTGGAATTGATACAGTTTTTAACCAAATCATTGATGAAGATCCTGAGGTTTTAAACTATTTAAAGCAAGTTAAAAAAGATCTTTCCAAAGAAAACAACGCTACATCCCAACTAACCTTTAATGTAACTATTAACCCTAAAAACACCCTCGCCAATTTTTTTGAAGGATTTAATATTTATCTCCATTTTAATGAAGAAAACAATACAGTGATAGGTAGTTTCTCACTACAATGA", "aa": "MIQKEMEIYNLFTFQIDLDKKLLFEKSNDQKNYSKIRTHYFKHKFKNKSAVFLNKNLIKNSLNKVLLNFSDFVSGAGIDTVFNQIIDEDPEVLNYLKQVKKDLSKENNATSQLTFNVTINPKNTLANFFEGFNIYLHFNEENNTVIGSFSLQ", "aa_hexdigest": "e5692d975c48de59b7a506c11193554a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17789.02409999999, "isoelectric_point": 8.669853401184085 }, "id": "KMADJIBOEG_547", "locus": "GCA000292445_00521" }, { "type": "cds", "sequence": "contig_1", "start": 286944, "stop": 287048, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATGAAATTAGTTTTATGCAGATCATTAATTGTTTTTCCAAAACAAAAATTAATAAACATGGTGAAATTGTTTTAAAAAGTTGTGCTTTTAAACAAAAATGA", "aa": "MHEISFMQIINCFSKTKINKHGEIVLKSCAFKQK", "aa_hexdigest": "3159e2c441e4613814c0f5d0ff1c4a2d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3982.7601000000013, "isoelectric_point": 9.512648582458496 }, "id": "KMADJIBOEG_548", "locus": "GCA000292445_00522" }, { "type": "cds", "sequence": "contig_1", "start": 287268, "stop": 287753, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGCTCTTTTTAAACGATTGGTACAACGAAAATCAAGCAATAACAACTGCTGACATTGAAAATGTTAATGAGGTTGAAGACATAGGAAAATTAGTTGAAAAATACAGCACTAATCAACCCCAAAAGTTATCGTTAAATTCAACAGTTTATGTATTACAAACAAAACAAAAACAGTTTTTTTTAAAAAATGATTTCTTTTTTAATAATAACGAAGCAAAGCTGTTTTTTCTAATAACTATGAAGCCCAATGTTTTTGGTCTTGATGATACAGCAATAGCAAATAACTTAAACCTTAAAAAAATTAGTGATTTTTTTAAAGAGATTGATTTTAATGATGAGGATATTCTAAATGACTTTAAACAAGAACAGGAAAAGTTATTGGTTAGAAGAACATTTAACCAACTGTTATTTATGAACAAAAATACTGAAATTCTCAGTGTTGTCAATGACAAGCAAAAGTCAGTAATCCATAACATTGTTTGA", "aa": "MLLFLNDWYNENQAITTADIENVNEVEDIGKLVEKYSTNQPQKLSLNSTVYVLQTKQKQFFLKNDFFFNNNEAKLFFLITMKPNVFGLDDTAIANNLNLKKISDFFKEIDFNDEDILNDFKQEQEKLLVRRTFNQLLFMNKNTEILSVVNDKQKSVIHNIV", "aa_hexdigest": "bd400d7f6bad3266f503ba3a15230cd8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18969.3497, "isoelectric_point": 4.9406953811645495 }, "id": "KMADJIBOEG_549", "locus": "GCA000292445_00523" }, { "type": "cds", "sequence": "contig_1", "start": 287778, "stop": 288266, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAAAGCCTTTGATTATTCCAAAGCCTTTGAAAAAAACCGAACGAGTGATCCTTCCTTATTGCGATCTAATTTAACTGTTATCAACCGCTTAAGATACCTCAGTGAATACTTTCAAAATGCCTCACTTAAGTATGATCTACTCTACACTAAGGCCAAACAATATATGCAAATTGATAAATTTATCAACGACATGATTCGCAAGGTAAACCATGAGGATGAAATCTTTGGCAAATTTAAAGAAAGAATTTATTTAAGTTTGGGAATTATTAGTGCGGTAGTGTTTGGCATAGTTGAATTTTTTAACTGTGTATGGACTATCTTAACTGTTAGTCAAGAAGTGGTTGATAAAAGTGTTTTGGATCCAAGGAATATTATCTTTATTAGTATAGGTACTATTTTGGTTTTATTTCTTTTGGTTACTATCTTGGTTTTTATGACAAGAAGACTTTATCTGTTTGAAATTAATAAAAAGCATAAAAATTAG", "aa": "MLKAFDYSKAFEKNRTSDPSLLRSNLTVINRLRYLSEYFQNASLKYDLLYTKAKQYMQIDKFINDMIRKVNHEDEIFGKFKERIYLSLGIISAVVFGIVEFFNCVWTILTVSQEVVDKSVLDPRNIIFISIGTILVLFLLVTILVFMTRRLYLFEINKKHKN", "aa_hexdigest": "5d8f68a7283e5c2c9c91b68ce463d716", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19152.35400000002, "isoelectric_point": 9.492792320251464 }, "id": "KMADJIBOEG_550", "locus": "GCA000292445_00524" }, { "type": "cds", "sequence": "contig_1", "start": 288272, "stop": 288829, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTCAATAAGTTAAACCTAAGTCATTGTATCTCTTTTTACATCTCAGAAGTTTCAGAAGTTTTCTTTGAAAGTATCAACCAACACCCTAGTCGTGATTTTGTTAATAACATTCTTCAAAAGATTAAAACTACTTTAAGTGAAGAAGAGCTTGAAAAGTTAAACAGTATTGAAGAAGTTACTAAGGATGAGAAGATTGTGATCATGCTCAACCATGTTTTGAAAAAGATTGTTTCTAAAACTGGTAGTTCTAAGTGTGACTTATTTAATGTAATCAAACAAGATAGGTTTAATTCTCCAGTGTATATCCAAAGTATTAATGCTTTTGAAAACAACCTTATTAACAATGAATTTGCTGAAAGAAGGTATGACTATTTGATTGAAGTTAATAAAAATTCATACCTTAAAAAATTTGTCAATTCGATCAGAATTTCCTTCTTCTTAGATTTAAGAGCACAAATTTTGTCAGGTTCATTTACCCTTAATTTAGTTAATAAGTCAATTGAAAAACAAAAAAGACAGAAATTTTTAAAGATATTTTTGTTAATGCCTTAG", "aa": "MKFNKLNLSHCISFYISEVSEVFFESINQHPSRDFVNNILQKIKTTLSEEELEKLNSIEEVTKDEKIVIMLNHVLKKIVSKTGSSKCDLFNVIKQDRFNSPVYIQSINAFENNLINNEFAERRYDYLIEVNKNSYLKKFVNSIRISFFLDLRAQILSGSFTLNLVNKSIEKQKRQKFLKIFLLMP", "aa_hexdigest": "f6168052d36bc75bb43ff150fe49d6b0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21783.00260000001, "isoelectric_point": 9.242977333068847 }, "id": "KMADJIBOEG_551", "locus": "GCA000292445_00525" }, { "type": "cds", "sequence": "contig_1", "start": 289030, "stop": 289248, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTATGCAGTGATTAACACTTATTTAATCATGTTAATCTCTGTTGAAGAGTTAAGGGTTTACTTTACAAGTAAAGAACCAGCACTGATTTTAAAGGTATTAGATAAAAAAAACACACTAAGAGAAGATCCTGATCAAAACCCTGAAACTGATTTGTATGAACTAATACAGTTTATAGAACAAAACTACTTAAAAAAAGATAAAAAAACCAGCTGA", "aa": "MFYAVINTYLIMLISVEELRVYFTSKEPALILKVLDKKNTLREDPDQNPETDLYELIQFIEQNYLKKDKKTS", "aa_hexdigest": "4ad88746209a892188341895700ea933", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8602.875899999995, "isoelectric_point": 5.017541694641112 }, "id": "KMADJIBOEG_552", "locus": "GCA000292445_00526" }, { "type": "cds", "sequence": "contig_1", "start": 289354, "stop": 289470, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAATTGATAATGATAATTTTGTCTCTACAAAACAAGTATTTAGAAACCAATTAGAATTACAACTTTTGCATGGGATTGTAATTAATCCTGAGAAGTATGGAATTGGCATGTGA", "aa": "MEIDNDNFVSTKQVFRNQLELQLLHGIVINPEKYGIGM", "aa_hexdigest": "253ffa8719db5ada7c9320d8eb02a9e2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4405.0166, "isoelectric_point": 4.895849418640137 }, "id": "KMADJIBOEG_553", "locus": "GCA000292445_00527" }, { "type": "cds", "sequence": "contig_1", "start": 289519, "stop": 289683, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCAATCCCAAAAGTGGTAATGATTTTTATCAGTTTGAAAAGGACATAGATGAAAGCATATGTCAAATTAACAAAAAATATCTAACTTTTATTAGCAGTGATAGTAATACCTTTTTAATAGTTAAAAATGATGATGTAAAAGTTATTTCTAACTATGTATGA", "aa": "MLNPKSGNDFYQFEKDIDESICQINKKYLTFISSDSNTFLIVKNDDVKVISNYV", "aa_hexdigest": "d29a41e46fa67643fa85e62367fc1e55", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6283.009700000002, "isoelectric_point": 4.663718986511231 }, "id": "KMADJIBOEG_554", "locus": "GCA000292445_00528" }, { "type": "cds", "sequence": "contig_1", "start": 289852, "stop": 290145, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGAAAATAGATAGTAAAAGTAATCTTAAAACCTCGATTGAAGAGATGGTAAATAGGTTTAAACATGAGCAGCGAATTAATAAAAAGGACAATGAAAGGGTGTTGATGATTTTTGCTTATATTGCTGCTTTTGTAGTAGGATTTATCAATTTTTTCTCAATGGTCTTTACTATTCTCACTGTAAGTGATCTAAATGCTGGGCTTACTGTACCTAACATCATTGTAATCAGTATCGCTAGTGTTTTAGCTTTTATTTTGATTGTGATTGCTGTTTTATTTCGTTTTAAATGA", "aa": "MQKIDSKSNLKTSIEEMVNRFKHEQRINKKDNERVLMIFAYIAAFVVGFINFFSMVFTILTVSDLNAGLTVPNIIVISIASVLAFILIVIAVLFRFK", "aa_hexdigest": "80a06bf5565933fbf5a52316216e565c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11056.121499999985, "isoelectric_point": 9.773100852966305 }, "id": "KMADJIBOEG_555", "locus": "GCA000292445_00529" }, { "type": "cds", "sequence": "contig_1", "start": 290475, "stop": 290846, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MG243", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47485", "UniRef:UniRef90_P47485" ], "nt": "ATGATCCATGAGTTGTTTTTATGGATTTTGCTTTCAGTTTTGTTTTTAGTGATTGCTAGTTATTTTTTTATTAACCAAAATAGCAGCGAAGCGCTTAATTTTTTCACTAATTTAGATAAGCCTAATGCAATAGCGATAACAATTAGAACCATAACAATTTTGATTAGTTTTTTACAACTGATTTTTATTGGTTATTTTTGTTTTAGTTCTGAAAAACAAGCTTTACAAGAGATACTGTCAAACACCTTTATGGTTCAAGAAAAACACACTTTAAAATCAAAACCAACTAGCTTAAAAACCAACAATCAACCTGATCCAGCTAATTTACCAGGCGTAATAGCTCTTGATGAAGTGGAAAAACTCATTAATTAA", "aa": "MIHELFLWILLSVLFLVIASYFFINQNSSEALNFFTNLDKPNAIAITIRTITILISFLQLIFIGYFCFSSEKQALQEILSNTFMVQEKHTLKSKPTSLKTNNQPDPANLPGVIALDEVEKLIN", "aa_hexdigest": "b0ea5b1cdebdf48a95766632e6b96074", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 290175, "stop": 290846, "observations": { "mutation": [ 290247, 290367 ], "stop": [ 290247, 290367 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47485", "query_cov": 1.0, "subject_cov": 0.5442477876106194, "identity": 1.0, "score": 228.0, "evalue": 3.28e-74, "gene_start": 1, "gene_end": 123, "reference_start": 104, "reference_end": 226, "reference_sequence": "MQIKVINETNKTVQIFQCAKVKHRALAWLCDVFLLAIVLVVIFLITQAFSDNRFLLFLVLSCSQTILWTVYFIFLPFFWDGKTLFRNLLKIKLFAFDKRFLRIMIHELFLWILLSVLFLVIASYFFINQNSSEALNFFTNLDKPNAIAITIRTITILISFLQLIFIGYFCFSSEKQALQEILSNTFMVQEKHTLKSKPTSLKTNNQPDPANLPGVIALDEVEKLIN" }, "paralog": false, "identity": 0.9910313901345291, "score": 429.0, "evalue": 1.09e-155, "description": "Internal stop codon at 290247, 290367. Nonsense mutation around 290247, 290367.", "psc": { "uniref90_id": "UniRef90_P47485", "product": "Uncharacterized protein MG243", "uniref50_id": "UniRef50_P47485", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47485", "UniRef:UniRef50_P47485", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47485", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47485" ], "product": "Uncharacterized protein MG243" } }, "id": "KMADJIBOEG_556", "locus": "GCA000292445_00530" }, { "type": "cds", "sequence": "contig_1", "start": 290852, "stop": 291616, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12", "PFAM:PF13245.12", "PFAM:PF13604.12", "PFAM:PF00580.27" ], "nt": "ATGAATGAACAACAAAAACAAGCAATTAGTTGTGGAAAAGGGGTTAATGTTGTTTATTCTGGAGCAGGTACTGGTAAAACAACAATTATTACTAATCGCTTTGCATACTTGGTTAATAAAGAAAAAGTTGATCCTAGCAGAATTTTAGCAATCACCTTTACTAAGAAAGCTGCTAAGGAGATGCAGTTTAGAATCTTGAAACTAATAGATAGTTCTTTAGCTGAGAAAACAAATATCTATACATTTCACAGCTTTTGCAATAAGTTTTTAATTCAAACATTAAAAAAGCGCTTTATCATCGATGATGATATTAGCTATTTCCTAAAGGAATTTTTAGCTGATTCAAAACTCGATATCAACCTAGCGAAACAAATTATTGATAACTTTAAAAATACTTTTGCTGATTTTGAAATAAATAAGTTGGATCAAGATGAAAGGTTAATTAGTTTATGTGAGCATTCACTTCTAAATAAAGATGAAGAATATTCCACTTTAAAAACCCAACTGATTAATGCATTCATTAGCTATGAAAAGAATAAGATATTAAACAATAAACTTGATTTTCATGATCTTTTAATTAAAACTTGTAATTTATTGAGTAATGATAATGATTTACTTAATCAGTGGAGTGAACAGTTTCAGCATATTTTAGTTGATGAATTTCAAGATACCAACCAAATCCAATATGAACTGATCAAGATGTTAGTAACTAAAAATAAAAACTTGTTTTTGGTAGGTGATAATAACCAGATGATTTACCGCTGA", "aa": "MNEQQKQAISCGKGVNVVYSGAGTGKTTIITNRFAYLVNKEKVDPSRILAITFTKKAAKEMQFRILKLIDSSLAEKTNIYTFHSFCNKFLIQTLKKRFIIDDDISYFLKEFLADSKLDINLAKQIIDNFKNTFADFEINKLDQDERLISLCEHSLLNKDEEYSTLKTQLINAFISYEKNKILNNKLDFHDLLIKTCNLLSNDNDLLNQWSEQFQHILVDEFQDTNQIQYELIKMLVTKNKNLFLVGDNNQMIYR", "aa_hexdigest": "734c02e5eed2df0bf894bfc136c2e196", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 263, "aa_cov": 0.9803149606299213, "hmm_cov": 0.9391634980988594, "evalue": 3.4612080300973125e-08, "score": 28.653270721435547, "start": 2, "stop": 250 }, { "id": "PF13245.12", "name": "AAA_19", "length": 253, "aa_cov": 0.9803149606299213, "hmm_cov": 0.5177865612648221, "evalue": 5.2491759740442314e-27, "score": 89.79872131347656, "start": 5, "stop": 253 }, { "id": "PF13604.12", "name": "AAA_30", "length": 65, "aa_cov": 0.24015748031496062, "hmm_cov": 0.9384615384615385, "evalue": 1.0329146765059335e-09, "score": 33.5244255065918, "start": 2, "stop": 62 }, { "id": "PF00580.27", "name": "UvrD-helicase", "length": 263, "aa_cov": 1.0, "hmm_cov": 0.9657794676806084, "evalue": 3.865124927145107e-77, "score": 254.6881561279297, "start": 1, "stop": 254 } ], "seq_stats": { "molecular_weight": 29636.69570000004, "isoelectric_point": 6.902265739440918 }, "id": "KMADJIBOEG_557", "locus": "GCA000292445_00531" }, { "type": "cds", "sequence": "contig_1", "start": 291755, "stop": 292114, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13361.12" ], "nt": "ATGGCCTATGAAAAACAAGTTAAAACTGAAAAAAATCTCTTGTTTTCAACTTTAAATTCTGATAAAAAACCTGTTTATTTTCAAGCTGAATCAGTTGAAAATCAAGCCAATTGGATCTTCAATAAAATCAAAGCACTAAACCAAACAGAAAAGATTAATTTTAAGGATATGGCCATCTTGTTTAGAAAGAACAGAGATATTACTACTATGGTTGAATTGATTGAAGCGGATGGAACAATTCCCTTACCCAAACAAAAGAGTTATTTTAACCAACTAGTAAAACTCCAGCGGGTTTTAATTGCGATTTCAACCAGAACAAATCTTGATATTAAAAGAGCTTTGCAAGCCCTAAAAATTTGA", "aa": "MAYEKQVKTEKNLLFSTLNSDKKPVYFQAESVENQANWIFNKIKALNQTEKINFKDMAILFRKNRDITTMVELIEADGTIPLPKQKSYFNQLVKLQRVLIAISTRTNLDIKRALQALKI", "aa_hexdigest": "427d93c4722c36a82a5ec96882383682", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13361.12", "name": "UvrD_C", "length": 72, "aa_cov": 0.6050420168067226, "hmm_cov": 0.9722222222222222, "evalue": 2.5002203710500295e-08, "score": 28.814903259277344, "start": 10, "stop": 81 } ], "seq_stats": { "molecular_weight": 13862.067299999977, "isoelectric_point": 9.800693321228025 }, "id": "KMADJIBOEG_558", "locus": "GCA000292445_00532" }, { "type": "cds", "sequence": "contig_1", "start": 292406, "stop": 292963, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13361.12", "PFAM:PF13538.12" ], "nt": "TTGGAATTTGACTCAATTATTGAAAACAGCAGTGATACAATCAATTTGCTAACCGTTCATGCAGCAAAAGGACTTGAGTTTGAAGCTGTATTTATTTATGGCATGAATCAAGGGGATTTTCCCTTATTTTTAAGTCAAAATCAAAATGACGAACAACATTTAATTGATGAATTAAAACTGTTTTATGTTGCTATCACAAGAGCAAAACGTTTTTTGTTTATCACTGCGGTTTTACAAATAAATAACAATTCTATAAAACCATCTAGTTTTTTAAATTACATCAATAAAAGTGAGTATTTAGACATTGCTACTATTAACTATGTATTAGAGCAGGATGATGATTTTTTTGATTCAACTAAAAAAACAGACTATACAAAGAAACTAAGAAAAGAAAGTTTAGACATTATAGTGGGTGATTTAGTTACTAGTAGATACTTTGGAAAAGGAGTTGTAGTTGAAGTGAGAGACAAAGAGGTTTTAGTAGCTTTTAAAGACACACGCTATGGGATGAAATGGATCTTAAAAAACCATAAATCACTAACAAAAGCTTTATATTAA", "aa": "MEFDSIIENSSDTINLLTVHAAKGLEFEAVFIYGMNQGDFPLFLSQNQNDEQHLIDELKLFYVAITRAKRFLFITAVLQINNNSIKPSSFLNYINKSEYLDIATINYVLEQDDDFFDSTKKTDYTKKLRKESLDIIVGDLVTSRYFGKGVVVEVRDKEVLVAFKDTRYGMKWILKNHKSLTKALY", "aa_hexdigest": "08120c03d7ba61bab0a9b782373cf333", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13361.12", "name": "UvrD_C", "length": 68, "aa_cov": 0.3675675675675676, "hmm_cov": 0.9705882352941176, "evalue": 9.269884654244673e-25, "score": 82.85357666015625, "start": 10, "stop": 77 }, { "id": "PF13538.12", "name": "UvrD_C_2", "length": 59, "aa_cov": 0.31891891891891894, "hmm_cov": 0.8135593220338984, "evalue": 2.609242204569004e-11, "score": 38.41843032836914, "start": 16, "stop": 74 } ], "seq_stats": { "molecular_weight": 21431.244100000004, "isoelectric_point": 5.657378196716309 }, "id": "KMADJIBOEG_559", "locus": "GCA000292445_00533" }, { "type": "cds", "sequence": "contig_1", "start": 292966, "stop": 293463, "strand": "+", "frame": 1, "gene": "fAU1", "product": "5-formyltetrahydrofolate cyclo-ligase", "db_xrefs": [ "COG:COG0212", "COG:H", "EC:6.3.3.2", "GO:0005524", "GO:0005737", "GO:0006730", "GO:0009396", "GO:0030272", "GO:0035999", "GO:0046872", "RefSeq:WP_009885782.1", "SO:0001217", "UniParc:UPI00001394EB", "UniRef:UniRef100_P47487", "UniRef:UniRef50_P75430", "UniRef:UniRef90_P47487" ], "nt": "ATGGTTGATAAAAACAGTTTAAGAAAATTAATGCTTCTAAAAAGAGCAGAACTAAATGATCTTGAAAAATCGCATTTAGATCAAAAGATTAACCAAAAATTAATGGCTTTTTTAATAACAAGACCAACAATTAAAAATTTAGCACTTTACATTCCCATTAAAAACGAAGTGGCTTTTTTAGATAACTTTCTAGATTTTCTTAAGTTAAATAAAATTACAAGCTGTTTTCCTAGTATTGTTGATCAATTTAACATGAAGTTTATTGATCAAAATAATAATGAAATTAACCCTAATGATATTGATTGTTTTTTTATCCCTTTATTAGCTTTTAATAAGGCAAACCACAGGATTGGTTTTGGTAAGGGTTATTATGACCGTTATTTATCATTAACTAGCAAAAAACAACTAAAAATAGGGATAGCATATGACTTTCAATATGCAGAATTCACTAATGATCCTTGGGATTATCAATTAGATTTAATTATTTGCAATGGATAA", "aa": "MVDKNSLRKLMLLKRAELNDLEKSHLDQKINQKLMAFLITRPTIKNLALYIPIKNEVAFLDNFLDFLKLNKITSCFPSIVDQFNMKFIDQNNNEINPNDIDCFFIPLLAFNKANHRIGFGKGYYDRYLSLTSKKQLKIGIAYDFQYAEFTNDPWDYQLDLIICNG", "aa_hexdigest": "a9380ce962d3874d46c708a41fac1d43", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394EB", "ncbi_nrp_id": "WP_009885782.1", "uniref100_id": "UniRef100_P47487", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394EB", "RefSeq:WP_009885782.1", "UniRef:UniRef100_P47487" ] }, "ips": { "uniref100_id": "UniRef100_P47487", "uniref90_id": "UniRef90_P47487" }, "psc": { "uniref90_id": "UniRef90_P47487", "gene": "FAU1", "product": "5-formyltetrahydrofolate cyclo-ligase", "ec_ids": [ "6.3.3.2" ], "uniref50_id": "UniRef50_P75430", "cog_id": "COG0212", "cog_category": "H", "go_ids": [ "GO:0005524", "GO:0005737", "GO:0006730", "GO:0009396", "GO:0030272", "GO:0035999", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P75430", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75430" ], "product": "5-formyltetrahydrofolate cyclo-ligase" }, "genes": [ "fAU1" ], "id": "KMADJIBOEG_560", "locus": "GCA000292445_00534" }, { "type": "cds", "sequence": "contig_1", "start": 293456, "stop": 293611, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13277.11" ], "nt": "ATGGATAACATAAAGGTTCTTTTTTTAGGTGATGTTTATGGCAAAGCTGGTAGAAAGATTATTAGTGATCATCTTCCCATAATTAAAAAAAAGTATCAGTTAAATCTAATTATTGCAAATGCTGAAAACACTACTAATGGTAAGGGTTTAAGTTGA", "aa": "MDNIKVLFLGDVYGKAGRKIISDHLPIIKKKYQLNLIIANAENTTNGKGLS", "aa_hexdigest": "fe570e65d19f9feb85cecc0e6cdc0fc2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13277.11", "name": "YmdB", "length": 44, "aa_cov": 0.8627450980392157, "hmm_cov": 1.0, "evalue": 3.819683393866398e-15, "score": 50.94171142578125, "start": 7, "stop": 50 } ], "seq_stats": { "molecular_weight": 5627.5596, "isoelectric_point": 9.698381996154787 }, "id": "KMADJIBOEG_561", "locus": "GCA000292445_00535" }, { "type": "cds", "sequence": "contig_1", "start": 293657, "stop": 294301, "strand": "+", "frame": 2, "gene": null, "product": "Putative metallophosphoesterase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PBN9" ], "nt": "ATGGGTAACCATACCTGGTTTCAAAAGCAAGATTTAGAACTTGTTTTAAACCAAGTTGATGTTATTCGCCCACTTAACTTAATGCAAGATTTTAACTATTTTCAGCTTGGCAAAGGGAGTTATTTATTTAGCTTAAATGGTTTGAAAATAAGGATTACTAACTTGTTAGGAACAAGTATTAACTTACCATTTGCAATAACAAACCCATTTGTGGAATTAAAAAAGTTAGTTTTAACTAAAGATTGTGATCTTCATATCGTTGATTTTCATGCTGAAACAACTAGCGAAAAAAATGCTTTTTGCATGGTTTTTGATGGTTATGTTACTGCTATCTTAGGAACCCACACCCATGTTCCTAGTAATGATTTAAGAATCACTCCTAAAGGAAGTGTTTACATTACTGATGTAGGGATGTGTGGTCCTGGATTTGGTAGTGTTATTGGTGCTAATCCCAAGCAATCAATTAAGTTATTTTGTACAGGTGAAAGACAGTTTTTTGAGGTTTCTAATTGTGGTGCACAACTCAATGGCGTCTTCTTTGAAGTTTGTTCAAAAACCAATCAAGTTGTGAAAATAGAACAGATTAGAATAGTTTTAGATGATGAAAAGTATTTAGCTAATGACTACTTTAATTTAGTTGAGTAA", "aa": "MGNHTWFQKQDLELVLNQVDVIRPLNLMQDFNYFQLGKGSYLFSLNGLKIRITNLLGTSINLPFAITNPFVELKKLVLTKDCDLHIVDFHAETTSEKNAFCMVFDGYVTAILGTHTHVPSNDLRITPKGSVYITDVGMCGPGFGSVIGANPKQSIKLFCTGERQFFEVSNCGAQLNGVFFEVCSKTNQVVKIEQIRIVLDDEKYLANDYFNLVE", "aa_hexdigest": "5ad3d6f535a230a8c00420df0697ca96", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PBN9", "query_cov": 0.8738317757009346, "subject_cov": 0.9540816326530612, "identity": 0.54, "score": 195.0, "evalue": 6.88e-60, "valid": false, "product": "Putative metallophosphoesterase", "ec_ids": [ "3.1.4.16" ], "uniref50_id": "UniRef50_A0A3B0PBN9", "kegg_orthology_id": "K09769" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PBN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PBN9" ], "product": "Putative metallophosphoesterase" }, "genes": [], "id": "KMADJIBOEG_562", "locus": "GCA000292445_00536" }, { "type": "cds", "sequence": "contig_1", "start": 294484, "stop": 294669, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTACAGCTAACACAAAGAATGTAATAAGACTTGCTAAAGAAACATACTTAGTTATTAAAGTAATCATTATCCAAATTAAAAGACAGATTAAAAATCATCATAAAGATATTGCAAGTAAAGATCCACCAGTAGTAGCAATTGCCTTACCACCCTTAAACTTAAAATACAGCGGAAAGATATGA", "aa": "MITANTKNVIRLAKETYLVIKVIIIQIKRQIKNHHKDIASKDPPVVAIALPPLNLKYSGKI", "aa_hexdigest": "0db4a42fbae561feb4819429db8ec9a4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6872.260500000002, "isoelectric_point": 10.237402153015136 }, "id": "KMADJIBOEG_563", "locus": "GCA000292445_00537" }, { "type": "cds", "sequence": "contig_1", "start": 294768, "stop": 295004, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCAAGCTAGTGCAATTGCCATTTTGGTCATTTTTAGCCTAGCTTCTGGTTATCTGTTAGGTTCAATTATTTTTGCTGATATTTTCAGCAAAATACTCAAGAAAAACGTCAGGGAATTTGGTTCAAAAAACCCAGGAGCTACTAACTCAATGCGTGTTTTTGGCTTAAAAATTGGTTTTTTGGTGGCTATTTTTGATGCATTTAAAGGTTTTTTTGCTTTTTTATTAACCTGA", "aa": "MNQASAIAILVIFSLASGYLLGSIIFADIFSKILKKNVREFGSKNPGATNSMRVFGLKIGFLVAIFDAFKGFFAFLLT", "aa_hexdigest": "7718ad0da02956cffe9c736a783e03c6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8469.010999999995, "isoelectric_point": 10.118586921691893 }, "id": "KMADJIBOEG_564", "locus": "GCA000292445_00538" }, { "type": "cds", "sequence": "contig_1", "start": 295167, "stop": 295646, "strand": "-", "frame": 2, "gene": "trmK", "product": "Putative tRNA methyltransferase MG248", "db_xrefs": [ "COG:COG2384", "COG:J", "EC:2.1.1.-", "GO:0005737", "GO:0008033", "GO:0032259", "GO:0160105", "KEGG:K06967", "SO:0001217", "UniRef:UniRef50_P47490", "UniRef:UniRef90_P47490" ], "nt": "ATGAAAAAAAGGATTAGCACAATTGCTAATTTAGTTCAATCATTTAATCCTAAGCTAGTGTATGACATCGGTTGTGATCATTCCTATCTAACATCCTATCTAATAAAAACTAATCAAAACCTAACCATTGTTAACAGTGACATTAGTAAAAATGCTTTATTAAGTAATTACCAAAAATTTAAAAATAACAATAATATCCACTTCTTTGTTAGTGATGGATTTAATAGCCTTCCAGAACTAAATATTAATCCCAAAATAGGGGTAATTGCTGGACTTGGTGGATTGAAAATCATTAACATAATTAGCCAAAAAGAAAACTTTATTAATCGTTTTGTAATCCAACCCCAATCTAATTTAATTGAATTACGTAGTTTTTTAAGTTTAAACAGTTGGGATATTGTTAATGAAACACTAGTTCAGGATCGAGAGTTTATATATCCTATTTTGGTTATTGAAAAACTAAAAAACCGTTTAAATTAA", "aa": "MKKRISTIANLVQSFNPKLVYDIGCDHSYLTSYLIKTNQNLTIVNSDISKNALLSNYQKFKNNNNIHFFVSDGFNSLPELNINPKIGVIAGLGGLKIINIISQKENFINRFVIQPQSNLIELRSFLSLNSWDIVNETLVQDREFIYPILVIEKLKNRLN", "aa_hexdigest": "737ed390dc38453ddf10cdbf3f508d76", "start_type": "ATG", "rbs_motif": null, "genes": [ "trmK" ], "truncated": "5-prime", "pseudogene": { "start": 294991, "stop": 295646, "observations": { "deletion": [ 295184 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47490", "query_cov": 0.9748427672955975, "subject_cov": 0.7110091743119266, "identity": 0.9940000000000001, "score": 301.0, "evalue": 1.31e-102, "gene_start": 1, "gene_end": 155, "reference_start": 1, "reference_end": 155, "reference_sequence": "MKKRISTIANLVQSFNPKLVYDIGCDHSYLTSYLIKTNQNLTIVNSDISKNALLSNYQKFKNNNNIHFFVSDGFNNLPELNINPKIGVIAGLGGLKIINIISQKENFINRFVIQPQSNLIELRSFLSLNSWDIVNETLVQDREFIYPILVIEKLKKPFKLTKELVILGPKLINFKDKHCLMKHYQCLLRVYQPKQKPSLMDLKIIETLNKIITSYESS" }, "paralog": false, "identity": 0.9954128440366973, "score": 427.0, "evalue": 1.84e-154, "description": "Frameshift due to deletion around 295184.", "psc": { "uniref90_id": "UniRef90_P47490", "gene": "TrmK", "product": "Putative tRNA methyltransferase MG248", "ec_ids": [ "2.1.1.-" ], "uniref50_id": "UniRef50_P47490", "cog_id": "COG2384", "cog_category": "J", "kegg_orthology_id": "K06967", "go_ids": [ "GO:0005737", "GO:0160105", "GO:0032259", "GO:0008033" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47490", "EC:2.1.1.-", "UniRef:UniRef50_P47490", "COG:COG2384", "COG:J", "KEGG:K06967", "GO:0005737", "GO:0160105", "GO:0032259", "GO:0008033" ] }, "pscc": { "uniref50_id": "UniRef50_P47490", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47490" ], "product": "Putative tRNA methyltransferase MG248" } }, "id": "KMADJIBOEG_565", "locus": "GCA000292445_00539" }, { "type": "cds", "sequence": "contig_1", "start": 295630, "stop": 296145, "strand": "-", "frame": 1, "gene": null, "product": "RNA polymerase sigma factor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EFD7AF2", "UniRef:UniRef90_UPI001EFD7AF2" ], "nt": "ATGGTAGAAACCATTAACCGCTTAGCTAAAGCAGAACGGGCTTTGTATCAAGAGTTAGGGCGAGAACCTACTGATGAGGAGTTAGCTGAAAAGATGGGAGGACAAGCTGAAGGATTTAATGTTAAAAAGATTGCTGAAATTAAACGGTTAAGTTTAGATCCAGTTTCGCTTGATAAAACAGTTGGACATGATGAAGAGTCCCAGTTTGGTGATTTTGTTAAAGACACAGACGCTCAAACTCCTGACGAGTTTACCGAAAGCCGTTCAAATTCAGAAAAAATTGATGAATTGTTGAACAATAATCTTTCTGAACAAGAAGAGTTAATTGTTAGAATGCGGATTGGCATGCCCCCTTACAATGAACCTAAAACACTTGATGAAGTAGGTCAAAAGATTTTGATCCCTAGAGAGAAGATCAGACAAATTGAAAACAAAGCAATTAGAAAATTGAGACATGCAGTTAGAAACAATCCTATTAGTATGTCATTTCTAAGAATTAATGAAAAAAAGGATTAG", "aa": "MVETINRLAKAERALYQELGREPTDEELAEKMGGQAEGFNVKKIAEIKRLSLDPVSLDKTVGHDEESQFGDFVKDTDAQTPDEFTESRSNSEKIDELLNNNLSEQEELIVRMRIGMPPYNEPKTLDEVGQKILIPREKIRQIENKAIRKLRHAVRNNPISMSFLRINEKKD", "aa_hexdigest": "becc7dd6d586894aa9b67a1b80d21d62", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 295630, "stop": 296445, "observations": { "mutation": [ 296214, 296250 ], "stop": [ 296214, 296250 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001EFD7AF2", "query_cov": 1.0, "subject_cov": 0.5245398773006135, "identity": 0.9009999999999999, "score": 281.0, "evalue": 1.27e-92, "gene_start": 1, "gene_end": 171, "reference_start": 156, "reference_end": 326, "reference_sequence": "MEEHIHEFRANQDLSIIDEDIEELTTKNISNRDKVDDNVRFFLGSLDSSKMLDFESEQRIAKVLNSTDEESRKYAINQLVTSNLRLVVSIAKKHLERGLDFNDLIQEGNLGLLKAISKFNWSLGNKFSTYATWWIKQAITRAIADQARTVRIPVHMVETINRLAKAERALNQELGREPTAEELAEKMGGQAEGFTVKKIAEIKRLSLDPVSLDKTVGHDEESQFGDFVRDTDAQMPDEFTESRSNYEKIDELLNNCLSEQEELIVRMRIGMPPYNETKTLDEVSQKIKIPREKIRQIETKAIRKLRQAVRNNHMSLSFMRGNEKKD" }, "paralog": false, "identity": 0.9298892988929889, "score": 478.0, "evalue": 5.91e-173, "description": "Internal stop codon at 296214, 296250. Nonsense mutation around 296214, 296250.", "psc": { "uniref90_id": "UniRef90_UPI001EFD7AF2", "product": "RNA polymerase sigma factor", "uniref50_id": "UniRef50_UPI001EFD7AF2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001EFD7AF2", "UniRef:UniRef50_UPI001EFD7AF2" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001EFD7AF2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EFD7AF2" ], "product": "RNA polymerase sigma factor" } }, "id": "KMADJIBOEG_566", "locus": "GCA000292445_00540" }, { "type": "cds", "sequence": "contig_1", "start": 296248, "stop": 296652, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04542.20" ], "nt": "TTGGATGAACTACGTGATAAGGGGATTCAACTCCAACACGATGTTGAAGAGCACATCCATGAATTTCGTGCTAACCAAGACCTTTCAATTATTGATGAAGATATTGAAGAGATAACAAGTAAGAACATCTCTAACCGTGATAAGGTTGATGATAATGTTAGGTTCTTTTTAGGATCACTTGACTTTTCTAAAATGTTAGATTTTGAATCTGAACAGCGGATTGCCAAGGTTTTAAATAGTACTGATGAAGAGTCACGTAAGTATGCAATTAATCAGTTGGTTACTTCAAACTTAAGACTAGTTGTTTCTATTGCCAAAAAACACCTAGAAAGAGGGTTGGATTTTAATGATTTAATTCAAGAGGGTAATTTGGGGCTTTTAAAAGCTATTTCCAAATTTAACTGA", "aa": "MDELRDKGIQLQHDVEEHIHEFRANQDLSIIDEDIEEITSKNISNRDKVDDNVRFFLGSLDFSKMLDFESEQRIAKVLNSTDEESRKYAINQLVTSNLRLVVSIAKKHLERGLDFNDLIQEGNLGLLKAISKFN", "aa_hexdigest": "08144e2be3a71868dc2637b10f5dd6dd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04542.20", "name": "Sigma70_r2", "length": 42, "aa_cov": 0.31343283582089554, "hmm_cov": 1.0, "evalue": 8.171025335327175e-13, "score": 43.178680419921875, "start": 93, "stop": 134 } ], "seq_stats": { "molecular_weight": 15517.207499999988, "isoelectric_point": 4.886641502380371 }, "id": "KMADJIBOEG_567", "locus": "GCA000292445_00541" }, { "type": "cds", "sequence": "contig_1", "start": 296758, "stop": 297123, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCCACTGACAAAAAAACGCTAGGCGAAAAACCCAATTCAACCAAACCAGAACTATCTGAAGAATTAATTGCTGAACTTAAAAAACAGCGTATTCTTGAAAAGAATCGTCCTTACAAAAAGATGATTTATGTTGACAATAAAGTGCAACGCAAACACCGTCATGAAAACATCGCTTTTCTCAAAACCCTTCATGAAAATAAGGAGAGTGATGTTCCTAAAAAAAGAAGGGGTAGAAAACCTAAACACGCTCCTTTAAAAGAAAAAAATAATCTGAAGTTATTTGATATCTTAGAAGGATCGTTAAAAAGCCACATTGAAAATGATGACACCAACACAGTCATCAACCTTCTAACAGAAGCTTGA", "aa": "MSTDKKTLGEKPNSTKPELSEELIAELKKQRILEKNRPYKKMIYVDNKVQRKHRHENIAFLKTLHENKESDVPKKRRGRKPKHAPLKEKNNLKLFDILEGSLKSHIENDDTNTVINLLTEA", "aa_hexdigest": "c31079dcb7bfcc478a226fe210da7c25", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14179.188199999977, "isoelectric_point": 9.724169349670408 }, "id": "KMADJIBOEG_568", "locus": "GCA000292445_00542" }, { "type": "cds", "sequence": "contig_1", "start": 297168, "stop": 297302, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAATTAAAGATATTTTTTTAGATGAATTAACTTGTTATCAAGCTAATGATTTTCAAAACTATCTAAAGACCTTTCAAACGTTATTAAAACAACAAAAGCAGCGCTTAAAAAATTTAAAGTTAACGCTATAA", "aa": "MEIKDIFLDELTCYQANDFQNYLKTFQTLLKQQKQRLKNLKLTL", "aa_hexdigest": "dccd2730aebfe8c31a4b258b9260da37", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5380.241800000001, "isoelectric_point": 8.889303779602052 }, "id": "KMADJIBOEG_569", "locus": "GCA000292445_00543" }, { "type": "cds", "sequence": "contig_1", "start": 297360, "stop": 297485, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTGAATTAAAACAATCCCATCTAGATGAAGTTTTTAAGGAATGTAACTTTAATTTGTTTGATGTTGAACTTTTTATTGAAAAAGCAAGGATCTATTGGAGTGAAAATCAAACTGCTAACTGA", "aa": "MVELKQSHLDEVFKECNFNLFDVELFIEKARIYWSENQTAN", "aa_hexdigest": "9a52b9846b11b0a8189fffd7a06df2f6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4979.554900000001, "isoelectric_point": 4.591988182067872 }, "id": "KMADJIBOEG_570", "locus": "GCA000292445_00544" }, { "type": "cds", "sequence": "contig_1", "start": 297528, "stop": 297728, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTAAACTACTTGAATATAGTGATGAAAAAACTCTGTATGAAACAGTTTTAAAACACAAAGAAAAACTTGTATCTAAGTTTGATAACAACCGTTTTTACATAAATACTTCAGGCCATGCTCAACCACCACAAGAATTGCAAAAAACCACTGCAGCACTAGTGCAAACAGCTTTTGAAGAAGCAGTTAATGAGTTGTGA", "aa": "MVKLLEYSDEKTLYETVLKHKEKLVSKFDNNRFYINTSGHAQPPQELQKTTAALVQTAFEEAVNEL", "aa_hexdigest": "6c3fd154940092a323ffeee1993b7cce", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7628.5366, "isoelectric_point": 5.585192680358887 }, "id": "KMADJIBOEG_571", "locus": "GCA000292445_00545" }, { "type": "cds", "sequence": "contig_1", "start": 297951, "stop": 298580, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08275.17", "PFAM:PF13362.12", "PFAM:PF13662.12" ], "nt": "ATGAATTATTTAGTTGAAAAAAGAAAGCTTAATAAAACATTAATTGAACAGTTTCAGCTAGGACTTGCTTTTCACAATGAAGATAAGTATCTATGTGAAAGTATGGAAAGATACCCCTTCATTAATCCAAAGATAAAACCGAGTGAATTGTATCTTTTTTCAAAAACTAACCAGCAAGGTCTTGGCTTTTTTGACTTTAATACCAAAAAAGCTACCTTTCAAAACCAGATTATGATCCCTATCCATGACTTTAATGGTAACCCGGTTGGTTTTTCAGCAAGAAGTGTTGATAACATCAACAAACTGAAATATAAAAATAGTGCTGATCACGAATTTTTTAAAAAAGGGGAGCTGTTATTTAACTTTCACAGGTTAAATAAAAACCTCAATCAACTCTTTATTGTGGAAGGTTATTTTGATGTTTTTACACTAACAAACTCCAAGTTTGAAGCTGTTGCATTAATGGGATTAGCATTAAATGATGTGCAAATTAAAGCCATTAAAGCTCACTTTAAGGAGTTACAAACCTTAGTTTTAGCACTTGATAATGATGCTAGTGGCCAAAATGCTGTGTTTAGCTTAATTGAAAAACTTAATAACAACAATTTTATTGTGGAAATTGTTCAGTGA", "aa": "MNYLVEKRKLNKTLIEQFQLGLAFHNEDKYLCESMERYPFINPKIKPSELYLFSKTNQQGLGFFDFNTKKATFQNQIMIPIHDFNGNPVGFSARSVDNINKLKYKNSADHEFFKKGELLFNFHRLNKNLNQLFIVEGYFDVFTLTNSKFEAVALMGLALNDVQIKAIKAHFKELQTLVLALDNDASGQNAVFSLIEKLNNNNFIVEIVQ", "aa_hexdigest": "4a2f6bb18690df8547ab732aa2c055b9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08275.17", "name": "DNAG_N", "length": 123, "aa_cov": 0.5885167464114832, "hmm_cov": 0.9024390243902439, "evalue": 1.0658880404211159e-22, "score": 75.69379425048828, "start": 2, "stop": 124 }, { "id": "PF13362.12", "name": "Toprim_3", "length": 81, "aa_cov": 0.3684210526315789, "hmm_cov": 0.9382716049382716, "evalue": 3.3093707971431205e-08, "score": 29.07499885559082, "start": 132, "stop": 208 }, { "id": "PF13662.12", "name": "Toprim_4", "length": 81, "aa_cov": 0.36363636363636365, "hmm_cov": 1.0, "evalue": 4.184548050762083e-13, "score": 44.54798126220703, "start": 131, "stop": 206 } ], "seq_stats": { "molecular_weight": 24185.49400000001, "isoelectric_point": 8.503782844543458 }, "id": "KMADJIBOEG_572", "locus": "GCA000292445_00546" }, { "type": "cds", "sequence": "contig_1", "start": 298749, "stop": 298991, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01807.25" ], "nt": "ATGGTCAATAAAAGCAACAGTTTAGATGAACTTTTAAAGCAGATTAAAATTACTGAAATTATTCAACACTACGGGGTTAAAATCCAAACTAAGGGTAATAGTCTACTTGCTTTATGTCCTTTTCATGATGATAAAAATCCTTCTATGTCCATTTCCAGTTCTAAAAACATCTTTAAGTGTTGGGCTTGTAATGCAGCTGGCAACGGAATAGCGTTTATCCAAAAGCATGACCAGTTAGATTGA", "aa": "MVNKSNSLDELLKQIKITEIIQHYGVKIQTKGNSLLALCPFHDDKNPSMSISSSKNIFKCWACNAAGNGIAFIQKHDQLD", "aa_hexdigest": "7ae53ff976d465a91306833323e38d78", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01807.25", "name": "Zn_ribbon_DnaG", "length": 76, "aa_cov": 0.95, "hmm_cov": 0.9868421052631579, "evalue": 5.99254984716995e-27, "score": 88.54666137695312, "start": 5, "stop": 80 } ], "seq_stats": { "molecular_weight": 8904.173199999994, "isoelectric_point": 8.50629711151123 }, "id": "KMADJIBOEG_573", "locus": "GCA000292445_00547" }, { "type": "cds", "sequence": "contig_1", "start": 299002, "stop": 299175, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25" ], "nt": "GTGTGCTTTGAGACAACTGGTAGTATTGGTAAAAGGTATCGAAAAGCAGATGCAATTGGAATAAAGTATGCAGTCACTTTTGACTTTGAAAGTTTAGAAGATAATGCAGTTACCATCAGAGATAGAGATACTTTAGTTCAACAGCGAATTGCTATCAAAGAATTACCACAATGA", "aa": "MCFETTGSIGKRYRKADAIGIKYAVTFDFESLEDNAVTIRDRDTLVQQRIAIKELPQ", "aa_hexdigest": "1f878184f893ddd4ec01356c38fbc426", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_F2R163", "query_cov": 0.9649122807017544, "subject_cov": 0.8333333333333334, "identity": 0.509, "score": 68.2, "evalue": 5.19e-14, "valid": false, "product": "Glycyl-tRNA synthetase", "uniref50_id": "UniRef50_A0A8T5UJC6" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 52, "aa_cov": 0.9122807017543859, "hmm_cov": 1.0, "evalue": 5.121448503549604e-16, "score": 53.81874465942383, "start": 5, "stop": 56 } ], "seq_stats": { "molecular_weight": 6523.367, "isoelectric_point": 6.138861274719238 }, "id": "KMADJIBOEG_574", "locus": "GCA000292445_00548" }, { "type": "cds", "sequence": "contig_1", "start": 299179, "stop": 299487, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTAACAGGGGTGATTTTGATCTAAAACAACACCAAGAGTTTTCAAAAAAGAGCATGAGTTTTTTTGATAGCGAATTAAACCAACATTTCTTACCTTTCATAATCGAACCTGCGGTTGGCATTGAACGGTTATTTTATGCACTAATTGTCAGTAGTTATAGGAGAGAAATTATTAATGAGGAAGAACGGGAAGTATTGAGTTTACCATTTGACTTATGTCCTGAACAAATTATTGTTTTACCACTTGTAAATAAACTTAAAAAAGAAGCATTTTCTGTATTTGAAACGCTAGCAAAAACAAGGTGA", "aa": "MANRGDFDLKQHQEFSKKSMSFFDSELNQHFLPFIIEPAVGIERLFYALIVSSYRREIINEEEREVLSLPFDLCPEQIIVLPLVNKLKKEAFSVFETLAKTR", "aa_hexdigest": "563bb330efc747edeb11aa1c89f3d862", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11980.710599999986, "isoelectric_point": 5.387790870666504 }, "id": "KMADJIBOEG_575", "locus": "GCA000292445_00549" }, { "type": "cds", "sequence": "contig_1", "start": 299491, "stop": 299928, "strand": "-", "frame": 1, "gene": null, "product": "aminoacyl--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0017CB9A86" ], "nt": "GTGAGGGATTTTAACTTACTTTTTCAAACTGAAATCGGGGTTGTAAACAACGATAAACGCCTTGTTTTTCTCCGTCCTGAGACTGCTCAAGGTAGCTTTATTAACTTTAAAAATATCTTGCAAGCTAAGAAGCGTAATTTACCTTTTGCTATTGCCCAGTTTGGTAAAAGCTTTCGTAATGAAATCACCCCAGGTAACTTCTTGTTTAGAACTAGAGAGTTTGAACAGTTTGAAATCGAGTGGTTTTGTAAACCTGATGATGCAAATTCGCTGTTTGAAAAACAATTAATAATGGTAGAACAGTTTCTACAAACAGTGTTAAAAATTAACCCAGAATTGTTAAAAAAACATGAATATGATCAATCAGAATTGGCTCATTATGCCAAAAAAACTACTGACTTTTTGTTTAATTTTCCCCACGGATTAAAGGAGTTATGA", "aa": "MRDFNLLFQTEIGVVNNDKRLVFLRPETAQGSFINFKNILQAKKRNLPFAIAQFGKSFRNEITPGNFLFRTREFEQFEIEWFCKPDDANSLFEKQLIMVEQFLQTVLKINPELLKKHEYDQSELAHYAKKTTDFLFNFPHGLKEL", "aa_hexdigest": "4c2afbe9d13e754f2886f84a1fdbfdf8", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0017CB9A86", "query_cov": 0.8137931034482758, "subject_cov": 0.8489208633093526, "identity": 0.534, "score": 137.0, "evalue": 5.23e-39, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "uniref50_id": "UniRef50_UPI0017CB9A86" }, "pscc": { "uniref50_id": "UniRef50_UPI0017CB9A86", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0017CB9A86" ], "product": "aminoacyl--tRNA ligase-related protein" }, "genes": [], "id": "KMADJIBOEG_576", "locus": "GCA000292445_00550" }, { "type": "cds", "sequence": "contig_1", "start": 300094, "stop": 300321, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAAGTTTACAACCAAGAAGTTTATGTTCAGTTTCTCAAACAACATGGTTTTGTATTTCAGAGTAGTGAAATTTACAACGGTTTAAACAATAGTTGGGATTTTGGTCCATTAGGTGCAGTTTTAAAACAACAAATCAAACAAGCTTTATATAACTTTTTTATTAAAAATAAAGCTGATGTTCTTTTAGTTGAAACCCCTATTATTCTCAGCGAATTGGTTTGA", "aa": "MAKVYNQEVYVQFLKQHGFVFQSSEIYNGLNNSWDFGPLGAVLKQQIKQALYNFFIKNKADVLLVETPIILSELV", "aa_hexdigest": "99c1362b2bb61d9d81f7f6d847e552ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8638.8954, "isoelectric_point": 6.534346961975097 }, "id": "KMADJIBOEG_577", "locus": "GCA000292445_00551" }, { "type": "cds", "sequence": "contig_1", "start": 300560, "stop": 300865, "strand": "-", "frame": 3, "gene": "rlmB", "product": "23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB", "db_xrefs": [ "COG:COG0566", "COG:J", "EC:2.1.1.-", "GO:0003723", "GO:0005737", "GO:0006396", "GO:0008173", "GO:0032259", "SO:0001217", "UniRef:UniRef50_P47494", "UniRef:UniRef90_P47494" ], "nt": "TTGGTAGCAGTTTTAGATACTAACCAACTCTTAATTCCACTTGATCAACTGGTTAAAGTAGTTGAAAATAAGAAGTGCAGTACACTTGTGATGTTAGATGAGATCCAAGACCCTTATAATTTTGGTGCTATACTAAGAACTTGTTTGGCTAGTGAGGTTGATGGAATTATCTTTAAAAAGAATAACCAAGTCCCTATCAACAATACAGTCATGAAAACTAGTATGGGTAGTGTTTTTTATCAAAACTTAGTGCAAGTAGCTAACTTAAGTTATACGATCACTAAGCTAAAAGAAATAGGATTTTGA", "aa": "MVAVLDTNQLLIPLDQLVKVVENKKCSTLVMLDEIQDPYNFGAILRTCLASEVDGIIFKKNNQVPINNTVMKTSMGSVFYQNLVQVANLSYTITKLKEIGF", "aa_hexdigest": "0ab4095d911ecfd522f47747da48ebf0", "start_type": "TTG", "rbs_motif": null, "genes": [ "rlmB" ], "truncated": "both", "pseudogene": { "start": 300341, "stop": 301069, "observations": { "mutation": [ 300352, 300532, 300562, 300904 ], "stop": [ 300352, 300532, 300562, 300904 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47494", "query_cov": 1.0, "subject_cov": 0.41735537190082644, "identity": 0.99, "score": 194.0, "evalue": 7.3e-61, "gene_start": 1, "gene_end": 101, "reference_start": 69, "reference_end": 169, "reference_sequence": "MKKPRQQSCLFGVKAFEEAINNQVHIKLVNISIRHKKLIPLIEAKKINFQIHSTNWFNNQYRDINHQELVAVLDTNQLLIPLDQLVKVVENKKCSTLVMLDEIQDPYNFGAILRTCLASEVDGIIFKKNNQVPINNTVMKTSMGSVFYQNLVQVANLSYTITKLKEIGFWTVVSTLDPIWKPIDYRKVDFAKKILIVGNEDRGVNQLITKNADCRIKIPMNNKINSLNVSVALGIILFAWKN" }, "paralog": false, "identity": 0.9834710743801653, "score": 462.0, "evalue": 6.58e-169, "description": "Internal stop codon at 300352, 300532, 300562, 300904. Nonsense mutation around 300352, 300532, 300562, 300904.", "psc": { "uniref90_id": "UniRef90_P47494", "gene": "rlmB", "product": "23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB", "ec_ids": [ "2.1.1.-" ], "uniref50_id": "UniRef50_P47494", "cog_id": "COG0566", "cog_category": "J", "go_ids": [ "GO:0003723", "GO:0005737", "GO:0006396", "GO:0008173", "GO:0032259" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47494", "EC:2.1.1.-", "UniRef:UniRef50_P47494", "COG:COG0566", "COG:J", "GO:0003723", "GO:0005737", "GO:0006396", "GO:0008173", "GO:0032259" ] }, "pscc": { "uniref50_id": "UniRef50_P47494", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47494" ], "product": "Uncharacterized tRNA/rRNA methyltransferase MG252" } }, "id": "KMADJIBOEG_578", "locus": "GCA000292445_00552" }, { "type": "cds", "sequence": "contig_1", "start": 300902, "stop": 301069, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACCACGCCAACAAAGTTGCTTATTTGGTGTTAAAGCCTTTGAGGAAGCGATTAATAACCAAGTTCACATTAAATTGGTAAACATTTCCATTCGTCACAAAAAACTAATACCTTTAATTGAAGCTAAAAAAATTAATTTTCAAATCCATTCAACTAACTGA", "aa": "MKKPRQQSCLFGVKAFEEAINNQVHIKLVNISIRHKKLIPLIEAKKINFQIHSTN", "aa_hexdigest": "81f499d59e3fd886272d7cc121b78df0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6395.550499999998, "isoelectric_point": 10.227280616760254 }, "id": "KMADJIBOEG_579", "locus": "GCA000292445_00553" }, { "type": "cds", "sequence": "contig_1", "start": 301411, "stop": 301671, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01406.26" ], "nt": "ATGGCTTTATATAACCAGCCCATTACCAAACATTGGATGCATATTGGTCATTTGATGATTGAAAACCAAAAGATGTCAAAGTCATTGCAGAACTTCTTGTTAGCAGTTGATTTTCTTAACTTTCATGATTTTCGTGTTTTGCGTTGGATCTTTTACCAAAAACACTATTTGCATCCTATTGATCTAAACCAATCATTGATTGAAAAAGCTAATAATGATATTCAAAGGATTGCAAAAACACTTAATGTTGCTAGAACCTGA", "aa": "MALYNQPITKHWMHIGHLMIENQKMSKSLQNFLLAVDFLNFHDFRVLRWIFYQKHYLHPIDLNQSLIEKANNDIQRIAKTLNVART", "aa_hexdigest": "9fbfdc96d5f93acce165d133e66bd98f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01406.26", "name": "tRNA-synt_1e", "length": 70, "aa_cov": 0.813953488372093, "hmm_cov": 1.0, "evalue": 5.9086333660152875e-24, "score": 80.05188751220703, "start": 2, "stop": 71 } ], "seq_stats": { "molecular_weight": 10375.995899999987, "isoelectric_point": 9.695351982116701 }, "id": "KMADJIBOEG_580", "locus": "GCA000292445_00554" }, { "type": "cds", "sequence": "contig_1", "start": 301843, "stop": 302382, "strand": "-", "frame": 1, "gene": "cysS", "product": "cysteine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.16", "GO:0004817", "GO:0005524", "GO:0005737", "GO:0006423", "SO:0001217", "UniRef:UniRef50_UPI001F010120", "UniRef:UniRef90_UPI001F010120" ], "nt": "ATGAAGCAATTTGAACATAGGTTTATGATTGTTGACAGTGTTAGTCAAAAACCAACAACACTAGTTCAAAAAACCATTAACATTTATCTCTGTGGACCCACAGTTTATAACGATTTGCACTTAGGCAACACCAGACCATTAATTGTTTTTGATGTTTTAAATAGAGTTTTAAAAAAGGCTAAATATACCGTTAATTTTGTTCAAAACATCACTGATATTGACGATAAGATCATCAAGATTGCTCAACAACAAGAAGTAAGCGAATCAGTTGTTACAAAACAACAAATCACTGCTTACAAATCACTTTTAAAAAAACTAAATATTCTGCCTATTAAACATATTCAAATCACTGAAAAAATCGATAAAATCCCTGACTATATTGATCAATTAGTAAATCAAAACCATGCTTATGTTTCAACTCAAAACAACGTTTATTTTGCAGTTAATTCACTAAAGCAATATGGTTATCTAGCTAACCGAATGGTGCATTTAGAAGAAACTGATACTGATAAAAAGAACAAATTGGATTTTGTACTTTGA", "aa": "MKQFEHRFMIVDSVSQKPTTLVQKTINIYLCGPTVYNDLHLGNTRPLIVFDVLNRVLKKAKYTVNFVQNITDIDDKIIKIAQQQEVSESVVTKQQITAYKSLLKKLNILPIKHIQITEKIDKIPDYIDQLVNQNHAYVSTQNNVYFAVNSLKQYGYLANRMVHLEETDTDKKNKLDFVL", "aa_hexdigest": "ebf4ad70eb3ca52b5351bb899bda96ef", "start_type": "ATG", "rbs_motif": null, "genes": [ "cysS" ], "truncated": "5-prime", "pseudogene": { "start": 301553, "stop": 302382, "observations": { "deletion": [ 301602 ], "mutation": [ 301785, 301818, 301845 ], "stop": [ 301563, 301785, 301818, 301845 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F010120", "query_cov": 1.0, "subject_cov": 0.6485507246376812, "identity": 1.0, "score": 350.0, "evalue": 9.99e-121, "gene_start": 1, "gene_end": 179, "reference_start": 1, "reference_end": 179, "reference_sequence": "MKQFEHRFMIVDSVSQKPTTLVQKTINIYLCGPTVYNDLHLGNTRPLIVFDVLNRVLKKAKYTVNFVQNITDIDDKIIKIAQQQEVSESVVTKQQITAYKSLLKKLNILPIKHIQITEKIDKIPDYIDQLVNQNHAYVSTQNNVYFAVNSLKQYGYLANRMVHLEETDTDKKNKLDFVLWKITTAGIKWNSKWGLGRPGWHVECAFLIDYCFKNELTIHGGGVDLKFPHHENENALHMALYNQPITKHWMHIGHLMIENQKDVKVIAELLVSSWFS" }, "paralog": false, "identity": 0.9855072463768116, "score": 540.0, "evalue": 8.2e-198, "description": "Internal stop codon at 301563, 301785, 301818, 301845. Frameshift due to deletion around 301602. Nonsense mutation around 301785, 301818, 301845.", "psc": { "uniref90_id": "UniRef90_UPI001F010120", "gene": "cysS", "product": "cysteine--tRNA ligase", "ec_ids": [ "6.1.1.16" ], "uniref50_id": "UniRef50_UPI001F010120", "go_ids": [ "GO:0004817", "GO:0005524", "GO:0005737", "GO:0006423" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F010120", "EC:6.1.1.16", "UniRef:UniRef50_UPI001F010120", "GO:0004817", "GO:0005524", "GO:0005737", "GO:0006423" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F010120", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F010120" ], "product": "cysteine--tRNA ligase" } }, "id": "KMADJIBOEG_581", "locus": "GCA000292445_00555" }, { "type": "cds", "sequence": "contig_1", "start": 302369, "stop": 303565, "strand": "-", "frame": 3, "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "db_xrefs": [ "COG:COG0272", "COG:L", "EC:6.5.1.2", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0006310", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47496", "UniRef:UniRef90_P47496" ], "nt": "TTGGAAGCGTTTGACAAAAAACGAGAACAGTTAACTTTTAATCTTGATGGTTTAGTTATTAAACTAAACAGCTTGCTTTTTTATCAACAATTAGGTGCTACAAGTAAATCACCACGTTGGGCAATAGCATTTAAATTTAGTCCTAAATTTGTTCAAACTAAATTAACAGCAGTTCTTATAACGATTGGTAGAACTGGTAGAGTGAACTATACTGCTAAATTAGAAAGTGTTAATTTAGATGGAACAAAAGTAACAGCTGCTACTTTACATAACTTTGATTACATTAAAACTAAAGACATTAGGATCAATGACACTGTTGTTATCTATAAAGCTGGGGAAATTATCCCTAAAGTACTAAAGGTAAACCTTGAAAAAAGAAAAAATGACACTATCATAATTCAAGAGCAAAAATATTGTCCTTCATGTAATTCAAAACTAGTCAAAATAGTTGATGAAGTTGATCAGTATTGTACCAATGAAACTTGTAAGGAGCGAAACATCCAGTTAATTAACTATTTTGTTTCTAAAACTGCTATGGACATTAACGGGTTGAATATTAATACTATTACCAAACTTTATGAACACAATTTGGTTAGATCTATAGTTGATCTTTATGATTTAAAAGACAAGAAAAACCAAGTTTTAAAATTAGATCTGAAGATTGGTGATAAACTTTTCAACAAGTTAGTTGATAACATTGAAAATTCAAAACAAAAAGGAATGGCTAGATTACTAACAGGACTTGGTATTAAGCATGTTGGTAGTGTATTAGCTAAGAATTTAGCTAATCATTTTAAAAATATCAAAGCATTACAGCATGCTAGCTTAGAGAACTTAATTAGTTTAAATGATGTAGGAATAACAGTAGCTGAATCATTGTATAACTGGTTTCATGACCCTAACCATTTGCAGTTAATTGAACAACTTGAATTAAGACAAGTAAAAACAGATCAATTACCACTGAAAATTAACTTTGAAACTAACAGTATTTATTTTCAAAAACGCTTTCTTATTACCGGTAGCTTTAACATTAGTCGTGACCAAATTAAGGATTTATTATCAGCTAAGTTTGATTGCCAGTTTGCAAGTGAAGTCAAACCAACAGTTGACTTTGTTATTGCAGGAAACAAACCAACTTTAAGAAAAATCAATCACGCCAAAGAACTGAACATTCCTATCATTAATGAAGCAATTTGA", "aa": "MEAFDKKREQLTFNLDGLVIKLNSLLFYQQLGATSKSPRWAIAFKFSPKFVQTKLTAVLITIGRTGRVNYTAKLESVNLDGTKVTAATLHNFDYIKTKDIRINDTVVIYKAGEIIPKVLKVNLEKRKNDTIIIQEQKYCPSCNSKLVKIVDEVDQYCTNETCKERNIQLINYFVSKTAMDINGLNINTITKLYEHNLVRSIVDLYDLKDKKNQVLKLDLKIGDKLFNKLVDNIENSKQKGMARLLTGLGIKHVGSVLAKNLANHFKNIKALQHASLENLISLNDVGITVAESLYNWFHDPNHLQLIEQLELRQVKTDQLPLKINFETNSIYFQKRFLITGSFNISRDQIKDLLSAKFDCQFASEVKPTVDFVIAGNKPTLRKINHAKELNIPIINEAI", "aa_hexdigest": "64ccdec450eb0aa16cd7fa1b97b671f4", "start_type": "TTG", "rbs_motif": null, "genes": [ "ligA" ], "truncated": "both", "pseudogene": { "start": 302363, "stop": 303865, "observations": { "mutation": [ 302371, 303628 ], "stop": [ 302371, 303628 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47496", "query_cov": 1.0, "subject_cov": 0.6039453717754173, "identity": 0.99, "score": 686.0, "evalue": 1.05e-243, "gene_start": 1, "gene_end": 398, "reference_start": 260, "reference_end": 657, "reference_sequence": "MMDVKLKIQQLVNLIKNYDYHYYVLSEPLIDDFEYDMLYKSLQQLEKDHPDLIQIDSPTQRVGGEAVKGFKKLNHNSPMLSLENAFSTKEIANFIDNINFQTNSKNEFVVEPKIDGVSISLTYKNGVLVHALTRGDGSVGEDVLNNVKTIKSIPLTIPFTKTIEIRGEIFVDKKTFLAINNQLEKPFANARNLAAGTIRNLNSEITAQRKLRALFYYIPNGLEESITTQTMVLEQLKQWKFPVSDTIRVFQNKFQLINYLEAFDKKREQLTFNLDGLVIKLNSLLFYQQLGATSKSPRWAIAFKFSPKFVQTKLTAVLITIGRTGRVNYTAKLESVNLDGTKVTAATLHNFDYIKTKDIRINDTVVIYKAGEIIPKVLKVNLEKRKNDTIIIQEQKYCPSCNSKLVKIVDEVDQYCTNETCKERNIQLINYFVSKTAMDINGLNINTITKLYEHNLVRSIVDLYDLKDKKNQVLKLDLKIGDKLFNKLVDNIENSKQKGMARLLTGLGIKHVGNVLAKNLANHFKNIKALQHASLENLISLNDVGITVAESLYNWFHDPNHLQLIEQLELRQVKTDQLPLKINFETNSIYFQKRFLITGSFNISRDQIKDLLSAKFDCQFASEVKPTVDFVIAGNKPTLRKINHAKELNIPIINEAIWT" }, "paralog": false, "identity": 0.99, "score": 882.0, "evalue": 0.0, "description": "Internal stop codon at 302371, 303628. Nonsense mutation around 302371, 303628.", "psc": { "uniref90_id": "UniRef90_P47496", "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "ec_ids": [ "6.5.1.2" ], "uniref50_id": "UniRef50_P47496", "cog_id": "COG0272", "cog_category": "L", "go_ids": [ "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0006310", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47496", "EC:6.5.1.2", "UniRef:UniRef50_P47496", "COG:COG0272", "COG:L", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0006310", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47496", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47496" ], "product": "DNA ligase" } }, "id": "KMADJIBOEG_582", "locus": "GCA000292445_00556" }, { "type": "cds", "sequence": "contig_1", "start": 303626, "stop": 304333, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01653.23" ], "nt": "GTGAAATTGAAGATTCAACAGCTGGTTAACTTAATAAAAAACTATGACTATCATTACTATGTTTTAAGCGAACCTTTAATTGATGATTTTGAGTATGATATGTTGTATAAGTCACTCCAACAATTAGAAAAAGATCATCCTGATTTAATCCAAATTGATTCCCCTACCCAAAGGGTGGGAGGAGAAGCTGTGAAGGGTTTTAAAAAGTTAAACCATAACAGTCCAATGCTCTCTTTGGAAAATGCTTTTTCAACTAAAGAAATTGCTAATTTTATTGATAATATTAACTTTCAAACAAACTCAAAAAATGAATTTGTAGTTGAACCTAAAATTGATGGAGTTAGTATCTCTCTAACTTATAAAAATGGTGTTTTAGTTCATGCTTTAACCAGAGGAGATGGAAGTGTTGGGGAAGATGTTTTAAATAATGTTAAAACCATTAAATCTATCCCTTTAACAATCCCTTTCACAAAAACAATTGAGATTAGGGGTGAGATTTTTGTTGATAAAAAAACTTTTTTAGCAATTAACAATCAACTTGAAAAACCATTTGCTAATGCAAGGAATCTAGCAGCAGGTACAATACGTAATTTAAACAGTGAAATCACTGCACAGCGCAAATTAAGGGCATTATTTTATTACATCCCTAATGGTTTGGAAGAGTCAATCACTACTCAAACTATGGTTTTAGAACAGCTTAAGCAGTGA", "aa": "MKLKIQQLVNLIKNYDYHYYVLSEPLIDDFEYDMLYKSLQQLEKDHPDLIQIDSPTQRVGGEAVKGFKKLNHNSPMLSLENAFSTKEIANFIDNINFQTNSKNEFVVEPKIDGVSISLTYKNGVLVHALTRGDGSVGEDVLNNVKTIKSIPLTIPFTKTIEIRGEIFVDKKTFLAINNQLEKPFANARNLAAGTIRNLNSEITAQRKLRALFYYIPNGLEESITTQTMVLEQLKQ", "aa_hexdigest": "c84fc75b289aeaf5b7e5ca9296fbf184", "start_type": "GTG", "rbs_motif": "ATTTA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01653.23", "name": "DNA_ligase_aden", "length": 181, "aa_cov": 0.723404255319149, "hmm_cov": 1.0, "evalue": 1.7848902465858587e-65, "score": 216.0919952392578, "start": 66, "stop": 235 } ], "seq_stats": { "molecular_weight": 26740.31080000003, "isoelectric_point": 6.446814918518065 }, "id": "KMADJIBOEG_583", "locus": "GCA000292445_00557" }, { "type": "tRNA", "sequence": "contig_1", "start": 304409, "stop": 304482, "strand": "-", "gene": "trnG", "product": "tRNA-Gly(gcc)", "amino_acid": "Gly", "anti_codon": "gcc", "score": 80.2, "nt": "GCAGATATAGTTCAATGGTAGAACATAACCTTGCCAAGGTTAAGATGCGGGTTCGATTCCCGTTATCTGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 304448, 304450 ], "id": "KMADJIBOEG_32", "locus": "GCA000292445_00558" }, { "type": "cds", "sequence": "contig_1", "start": 304518, "stop": 304811, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATCTGAAAACCAAATTGCAATCCTGGATTATATTTTTAACCAAGTCAACCAGCCCAATCAACCAAAAATAGTTTGGTTCTCTGGGGAAGGGGAGGATGAGAAAATTAATTTTTTAATCCGCTTAAATGATTTTTTTAAACCAAAATTTGTTGAAAATACTAATGATAGTAGCTTTTTATTAAGTTTTAGAAACCATGTTGAAACTAAAAATTCAACGCCTTTAACCCAAGCTAACTTTGCTAATATTGCCAACAAATTACTAGCGGTTTTATTTGGTTCATTGCAATGA", "aa": "MESENQIAILDYIFNQVNQPNQPKIVWFSGEGEDEKINFLIRLNDFFKPKFVENTNDSSFLLSFRNHVETKNSTPLTQANFANIANKLLAVLFGSLQ", "aa_hexdigest": "8442183bc8b656aa3f9e060de47a4d1b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11153.428599999983, "isoelectric_point": 4.967807579040527 }, "id": "KMADJIBOEG_584", "locus": "GCA000292445_00559" }, { "type": "cds", "sequence": "contig_1", "start": 304941, "stop": 305681, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MG255", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47497", "UniRef:UniRef90_P47497" ], "nt": "TTGAGTTTTATTAAACAACAATGGCCTATTCTAACAACATTGGTAACAGTAGGGACCACATTAGGTACTCCAGTATTTTCATTAACAATAGCCCAACAAGATGGTATTAAGCAAAATGCGGGGAATGATGTCTTTATCTTTTTGATTATCTTCTCTGTTTTTTCTATTAGTTTAGGGCTTGTTTCATCACTTATCTTTTTAGTCTCCAGCTTATTTTCAATTCGTCAGAAAAAAACGTTGGATGCTTTGGATAAAGTGTTATCAAAATTTATTGATAAATACTTTTTTTTAGATGAAAAAGAGATCAAAAAACAACTTAAATATCAGTTTAAAAACAACGGTGTTTGCTTTTTTTATGGTTTTGATTTTGATCAGGCAGAGTTTCTTGAACAATCAATGAACTTAATGTTGTTGTTAAAGCAAACCAATTGTTTTATTTTAGTTGGTTGTAAAGAAAGTGAAATGACGCTCATTAAAAACAAGATAGAACCTAACATTAACTTAAAACAAAACAGTTTTTATCTGGATTTAAGCAATGAAATTTCACAAGTAGAACAGATCAGCAAATTTAACTTGTTGTTTAGGCAATTGAGGCTCAGTTCTGAACTATTTTACTTAGAAGATTTTTTTGATTATCTGACTACTGCAAAACAGATAGTTAACTTTCTTTTTAAAACAAAACCTAACCTTGATCAATTTCAAGAAAATCAAAAGAAATTATTTGATTTTTTAGCGTTATGA", "aa": "MSFIKQQWPILTTLVTVGTTLGTPVFSLTIAQQDGIKQNAGNDVFIFLIIFSVFSISLGLVSSLIFLVSSLFSIRQKKTLDALDKVLSKFIDKYFFLDEKEIKKQLKYQFKNNGVCFFYGFDFDQAEFLEQSMNLMLLLKQTNCFILVGCKESEMTLIKNKIEPNINLKQNSFYLDLSNEISQVEQISKFNLLFRQLRLSSELFYLEDFFDYLTTAKQIVNFLFKTKPNLDQFQENQKKLFDFLAL", "aa_hexdigest": "08e6ec7b7a9bbcca720cb0d5c2d0d01e", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 304641, "stop": 305615, "observations": { "mutation": [ 304809, 304839, 304887 ], "stop": [ 304809, 304839, 304887 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47497", "query_cov": 0.9105691056910569, "subject_cov": 0.6136986301369863, "identity": 0.996, "score": 420.0, "evalue": 9.39e-146, "gene_start": 1, "gene_end": 224, "reference_start": 142, "reference_end": 365, "reference_sequence": "MESENQIAILDYIFNQVNQPNQPKIVWFSGEGEDEKINFLIRLNDFFKPKFVENTNDSSFLLSFRNHVETKNSTPLTQANFANIANKLLAVLFGSLQWKQLNKPTGNWFLVILFLALLWLRQCWLKLQLTKISKFVNQKGILSFIKQQWPILTTLVTVGTTLGTPVFSLTIAQQDGIKQNAGNDVFIFLIIFSVFSISLGLVSSLIFLVSSLFSIRQKKTLDALDKVLSKFIDKYFFLDEKEIKKQLKYQFKNNGVCFFYGFDFDQAEFLEQSMNLMLLLKQTNCFILVGCKESEMTLIKNKIEPNINLKQNSFYLDLSNEISQVEQISKFNLLFRQLRLSSELFYLEDFFDYLTTAKQIVNFLF" }, "paralog": false, "identity": 0.9907407407407407, "score": 622.0, "evalue": 5.44e-228, "description": "Internal stop codon at 304809, 304839, 304887. Nonsense mutation around 304809, 304839, 304887.", "psc": { "uniref90_id": "UniRef90_P47497", "product": "Uncharacterized protein MG255", "uniref50_id": "UniRef50_P47497", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47497", "UniRef:UniRef50_P47497", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47497", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47497" ], "product": "Uncharacterized protein MG255" } }, "id": "KMADJIBOEG_585", "locus": "GCA000292445_00560" }, { "type": "tRNA", "sequence": "contig_1", "start": 306133, "stop": 306207, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(cct)", "amino_acid": "Arg", "anti_codon": "cct", "score": 54.4, "nt": "CTTCCCTTGGTGCAATGGACAGCACAACTGAGTCCTAATCAGTAAATAGAGGTTCAACTCCTCTAGGGAAGGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 306137, 306139 ], "id": "KMADJIBOEG_10", "locus": "GCA000292445_00561" }, { "type": "tRNA", "sequence": "contig_1", "start": 306256, "stop": 306329, "strand": "+", "gene": "trnW", "product": "tRNA-Trp(cca)", "amino_acid": "Trp", "anti_codon": "cca", "score": 75.5, "nt": "GGGGGTGTAGTTTAGTGGTAGAACAACAGTCTCCAAAACTGTCTGTGTGGGTTCGATTCCTTCCACCCCCGCCA", "db_xrefs": [ "SO:0000271" ], "anti_codon_pos": [ 306288, 306290 ], "id": "KMADJIBOEG_11", "locus": "GCA000292445_00562" }, { "type": "cds", "sequence": "contig_1", "start": 306337, "stop": 306516, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCACTTCAATTCAAACTTTAAAGAACGCTTTAATAAGATTGCAAAAAAAGTTAATTCACTGGACAGTGAATACTATGAGTTTTCTTCTTTTATTGAAAGGATTAGAACTACCTTTGGACTTTTAATTGCTTTAACTGTTTTAAGTAACCTAATCATTATTAGCTTTGTTTTAATTTGA", "aa": "MHFNSNFKERFNKIAKKVNSLDSEYYEFSSFIERIRTTFGLLIALTVLSNLIIISFVLI", "aa_hexdigest": "6e8559266a6db41d3163c11aae0ea9c0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6933.033199999994, "isoelectric_point": 9.300805473327635 }, "id": "KMADJIBOEG_586", "locus": "GCA000292445_00563" }, { "type": "cds", "sequence": "contig_1", "start": 306718, "stop": 306858, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGATTAAATAAAACTATTAAGAATTTCGCTAGTGATTATGGTTTTAAGATTGTTAATAAATATAACAGTTTAACAAGCGGAATTTTTGTGTTTGACTTTGCTGATTATGTTAGTTTTGAAGCCAACCTGACTAATTGA", "aa": "MRLNKTIKNFASDYGFKIVNKYNSLTSGIFVFDFADYVSFEANLTN", "aa_hexdigest": "9349d36f0229c30105ecd7763246f589", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5304.936200000001, "isoelectric_point": 8.063141441345213 }, "id": "KMADJIBOEG_587", "locus": "GCA000292445_00564" }, { "type": "cds", "sequence": "contig_1", "start": 307153, "stop": 307455, "strand": "+", "frame": 1, "gene": "rpmE", "product": "50S ribosomal protein L31", "db_xrefs": [ "COG:COG0254", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "SO:0001217", "UniRef:UniRef50_P47499", "UniRef:UniRef90_P47499" ], "nt": "ATGAAAAAGGCAATCCACTTTCAGAGTCAACCAGTTGTTTTTAACTGTGCTTCATGCAATAGCAACTTTACCATTGACTCCACTGCCAAACAAAAGGATCTTGCCATTGACATTTGTGGAAAATGTCATCCTTTTTACATAGGGCAATTAACCAAACAAACCGTGCATGGACGGGCTGAAAAACTTTCTCAAAAGTTCAACGCTGGAAAGGCTTTTTTAGAAAAAAAACTAAAAAGAGTAACCAAGCTAAAGTTGAAAAACAAACTAGGCACCGTTCTATTAACGAGCTTTAGTGGATTTTGA", "aa": "MKKAIHFQSQPVVFNCASCNSNFTIDSTAKQKDLAIDICGKCHPFYIGQLTKQTVHGRAEKLSQKFNAGKAFLEKKLKRVTKLKLKNKLGTVLLTSFSGF", "aa_hexdigest": "310e41b90d0c1a3477a02d62816ae837", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47499", "query_cov": 0.85, "subject_cov": 0.8762886597938144, "identity": 0.9059999999999999, "score": 160.0, "evalue": 2.61e-49, "valid": true, "gene": "rpmE", "product": "50S ribosomal protein L31", "uniref50_id": "UniRef50_P47499", "cog_id": "COG0254", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P47499", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47499" ], "product": "Large ribosomal subunit protein bL31" }, "genes": [ "rpmE" ], "id": "KMADJIBOEG_588", "locus": "GCA000292445_00565" }, { "type": "cds", "sequence": "contig_1", "start": 307445, "stop": 307885, "strand": "+", "frame": 2, "gene": "prfA", "product": "peptide chain release factor 1", "db_xrefs": [ "COG:COG0216", "COG:J", "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149", "KEGG:K02835", "SO:0001217", "UniRef:UniRef50_P47500", "UniRef:UniRef90_P47500" ], "nt": "GTGGATTTTGACAAACAACTCTTTTTCAATGTTGAAAAGATTGTTGAACTTACTGAACAACTTGAAAAAGATCTCAATAAACCTAACCTTAGCTTTGAGCAGATTAAAGTTATTAACAAAGAGTTAAAACATAAACAACCTTTAATAGTTAAATTCAAGGAGTTGCAAAAGCTGGTTGAAAATGCTAATGAAGCTGAACAAATTCTTAACAATTCCAGCTTAAAAGAATTGCATGAAGAAGCTAAAAAAGAACTTGAAAAAATCAAAGCTAGTTTACCTAGTTTAGAAGAGGAGATTAAGTTCCTTTTACTACCGGTAGATGAAAATAACCAAAAGAATGTTATTGTAGAGATCCGTCCAGCTGCTGGTGGGGATGAATCTTGCATCTTCTTAAGTGATCTTTTTAATATGTACAAAAACTATTGTACTAGTAAAAATTGA", "aa": "MDFDKQLFFNVEKIVELTEQLEKDLNKPNLSFEQIKVINKELKHKQPLIVKFKELQKLVENANEAEQILNNSSLKELHEEAKKELEKIKASLPSLEEEIKFLLLPVDENNQKNVIVEIRPAAGGDESCIFLSDLFNMYKNYCTSKN", "aa_hexdigest": "79b475bf50048d446eca0dccdb6baad5", "start_type": "GTG", "rbs_motif": "TTCTA", "genes": [ "prfA" ], "truncated": "3-prime", "pseudogene": { "start": 307445, "stop": 308188, "observations": { "mutation": [ 307883 ], "stop": [ 307883 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47500", "query_cov": 1.0, "subject_cov": 0.40668523676880225, "identity": 1.0, "score": 273.0, "evalue": 1.4e-89, "gene_start": 1, "gene_end": 146, "reference_start": 1, "reference_end": 146, "reference_sequence": "MDFDKQLFFNVEKIVELTEQLEKDLNKPNLSFEQIKVINKELKHKQPLIVKFKELQKLVENANEAEQILNNSSLKELHEEAKKELEKIKASLPSLEEEIKFLLLPVDENNQKNVIVEIRPAAGGDESCIFLSDLFNMYKNYCTSKNWTVELNEIIPASVGINFVSFAVNGTDVFAKLKFESGVHRVQRVPLTEAKGRVHTSTVTVAVLPQLEEVEITINPSDLRIDTYRASGAGGQHVNRTESAVRITHLPTGIVVACQEGKSQFSNRDKAMKMLRAKLWENAQNKQLSTQADLRKSQVGSGERAEKIRTYNYPQNRITDHRIKLTINKLNTVILGDLDEIIEALQADEKKQQLEKFIS" }, "paralog": false, "identity": 0.9919028340080972, "score": 475.0, "evalue": 1.21e-171, "description": "Internal stop codon at 307883. Nonsense mutation around 307883.", "psc": { "uniref90_id": "UniRef90_P47500", "gene": "prfA", "product": "peptide chain release factor 1", "uniref50_id": "UniRef50_P47500", "cog_id": "COG0216", "cog_category": "J", "kegg_orthology_id": "K02835", "go_ids": [ "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47500", "UniRef:UniRef50_P47500", "COG:COG0216", "COG:J", "KEGG:K02835", "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149" ] }, "pscc": { "uniref50_id": "UniRef50_P47500", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47500" ], "product": "Peptide chain release factor 1" } }, "id": "KMADJIBOEG_589", "locus": "GCA000292445_00566" }, { "type": "cds", "sequence": "contig_1", "start": 307919, "stop": 308524, "strand": "+", "frame": 2, "gene": "prfA", "product": "peptide chain release factor 1", "db_xrefs": [ "COG:COG0216", "COG:J", "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149", "KEGG:K02835", "SO:0001217", "UniRef:UniRef50_P47500", "UniRef:UniRef90_P47500" ], "nt": "GTGGGGATTAACTTTGTTTCTTTTGCGGTAAATGGTACTGATGTTTTTGCTAAACTCAAGTTTGAATCAGGAGTACATAGGGTGCAACGTGTTCCTTTAACAGAAGCTAAAGGTAGAGTGCATACCTCAACAGTTACTGTTGCTGTTTTACCTCAATTAGAAGAGGTAGAGATCACCATTAATCCTAGTGATTTGAGAATCGATACTTATCGTGCTTCAGGAGCGGGTGGTCAACATGTTAACAGAACTGAAAGTGCAGTTAGAATTACCCATCTACCTACGGGAATTGTTGTGGCTTGCCAAGAAGGTAAATCCCAGTTTTCTAACCGTGATAAAGCAATGAAAATGCTACGTGCTAAGTTATGGGAAAATGCTCAAAATAAACAACTCTCAACCCAAGCGGATTTAAGAAAAAGTCAAGTTGGTAGTGGAGAGAGGGCTGAGAAAATTCGTACCTACAACTATCCCCAAAACAGAATTACAGACCACAGGATTAAATTAACTATTAATAAGCTTAATACTGTTATTTTGGGAGATCTTGATGAGATCATTGAAGCTTTACAAGCTGATGAAAAGAAACAACAGTTGGAAAAGTTTATTAGTTAG", "aa": "MGINFVSFAVNGTDVFAKLKFESGVHRVQRVPLTEAKGRVHTSTVTVAVLPQLEEVEITINPSDLRIDTYRASGAGGQHVNRTESAVRITHLPTGIVVACQEGKSQFSNRDKAMKMLRAKLWENAQNKQLSTQADLRKSQVGSGERAEKIRTYNYPQNRITDHRIKLTINKLNTVILGDLDEIIEALQADEKKQQLEKFIS", "aa_hexdigest": "c2cc6985177949825b26e2cd5375f320", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_K1ZX70", "query_cov": 0.9552238805970149, "subject_cov": 0.8421052631578947, "identity": 0.62, "score": 246.0, "evalue": 7.87e-80, "valid": false, "product": "Peptide chain release factor 1", "uniref50_id": "UniRef50_A0A2C9JR53", "kegg_orthology_id": "K02835" }, "genes": [ "prfA" ], "truncated": "5-prime", "pseudogene": { "start": 307619, "stop": 308524, "observations": { "mutation": [ 307883 ], "stop": [ 307883 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47500", "query_cov": 1.0, "subject_cov": 0.5598885793871866, "identity": 0.995, "score": 386.0, "evalue": 3e-133, "gene_start": 1, "gene_end": 201, "reference_start": 159, "reference_end": 359, "reference_sequence": "MDFDKQLFFNVEKIVELTEQLEKDLNKPNLSFEQIKVINKELKHKQPLIVKFKELQKLVENANEAEQILNNSSLKELHEEAKKELEKIKASLPSLEEEIKFLLLPVDENNQKNVIVEIRPAAGGDESCIFLSDLFNMYKNYCTSKNWTVELNEIIPASVGINFVSFAVNGTDVFAKLKFESGVHRVQRVPLTEAKGRVHTSTVTVAVLPQLEEVEITINPSDLRIDTYRASGAGGQHVNRTESAVRITHLPTGIVVACQEGKSQFSNRDKAMKMLRAKLWENAQNKQLSTQADLRKSQVGSGERAEKIRTYNYPQNRITDHRIKLTINKLNTVILGDLDEIIEALQADEKKQQLEKFIS" }, "paralog": false, "identity": 0.9966777408637874, "score": 583.0, "evalue": 3.63e-213, "description": "Internal stop codon at 307883. Nonsense mutation around 307883.", "psc": { "uniref90_id": "UniRef90_P47500", "gene": "prfA", "product": "peptide chain release factor 1", "uniref50_id": "UniRef50_P47500", "cog_id": "COG0216", "cog_category": "J", "kegg_orthology_id": "K02835", "go_ids": [ "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47500", "UniRef:UniRef50_P47500", "COG:COG0216", "COG:J", "KEGG:K02835", "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149" ] }, "pscc": { "uniref50_id": "UniRef50_P47500", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47500" ], "product": "Peptide chain release factor 1" } }, "id": "KMADJIBOEG_590", "locus": "GCA000292445_00567" }, { "type": "cds", "sequence": "contig_1", "start": 308525, "stop": 308650, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCCTGTATGAGTTTTTTTTAAATCAAAAGTTAGTTTACCAATCCAGTCCCCATTTTAACGGGGTATTTTTAACAATATTGGAACACTATGGTTTTCAATTTAAAACAATTGATAAACTCTGA", "aa": "MTLYEFFLNQKLVYQSSPHFNGVFLTILEHYGFQFKTIDKL", "aa_hexdigest": "dbaef87e3742785d0b97ab90629f305c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4958.684300000001, "isoelectric_point": 6.697759056091309 }, "id": "KMADJIBOEG_591", "locus": "GCA000292445_00568" }, { "type": "cds", "sequence": "contig_1", "start": 309133, "stop": 309564, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTTTTCAAAATAAAAATAGTTATTTTGCATACAAGCAGTTGTTTAATCTATTACTAACAAAACGATCAATTAAACAGTTAATTTTTGAATGTTCTTTATTTCAAAAAGAAAGGCTATTAAATTTGTTTTCAATCTTTAAATCAAGGCCGATTTTTAACTTTCAAAAACAGTTTATTGGTATGAAAGTTGATAATCAAAAACTCCCAGTAGTTGATATTAAAAATACCAAAACTATTAAGCAACTTTTAAAAATGGGGCTAGCAGGAATTGTAAATACTGATACACAAATGGGATTAATTAGTTATTCAGAGTCTACTCTTGACAAAATTAAACAACGTGCACTTAACAAACATTATGTATCAATGTTTGGGTTAGAAGAATTAAAGAAGTTACCAAAAAAACTACAACAAATTGCTAGTTACTTTTGA", "aa": "MVFQNKNSYFAYKQLFNLLLTKRSIKQLIFECSLFQKERLLNLFSIFKSRPIFNFQKQFIGMKVDNQKLPVVDIKNTKTIKQLLKMGLAGIVNTDTQMGLISYSESTLDKIKQRALNKHYVSMFGLEELKKLPKKLQQIASYF", "aa_hexdigest": "0149733c06491adca5ee57a3bf28c3e5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16795.792999999983, "isoelectric_point": 10.03767910003662 }, "id": "KMADJIBOEG_592", "locus": "GCA000292445_00569" }, { "type": "cds", "sequence": "contig_1", "start": 310025, "stop": 310660, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGCTCAAAGGTTTTTTAGCTGTTGGTGTTAGTGTTTTTGGTTTTTCTGGTTTACTGATGGCTTGTAGTGTTGTAAGTCAGTTTGATCAAGTGGATGATGGCAAAATTAAGCTAGCATCTTCATTAACTTCGAAACGCGCAGCGGAAGCTTTAGAGACAGTAGTTAAAAAATATAACGATACTAAAGATCCTGGTGATTATCCAATTGAAATAGTACAAATTGCTGGGGGTTATGATGGGGGTAAAAAGGATGTTCAAACCAAGGTTAGTACCAAAGATAAAAATAACTTTTATAACTTGATTTTAAACTATCCAGAAATAGTAAGTACCTTATCAAGGTCTAAAATGGCTTTGAATTTTGATGGGATTAATGTTGATAAACTCCATCCTAACTTTTTAAGCTTTAACAGCAGAATAGGTGGAATTAGAGATGATGGAATCTATGCTATTCCAATATCTATGTCCACTGATCTGATGGTCATTAATGGCCCTGTTTTACACTATATTCTAAACAGTGCTAGAAAAGAAGGTACACCAACTAGCACTACTGTTCAAGCAACTGTCAGTTCAAGAAGTGCAGAAAAAAAAGGTACATTAGAAATTGCAAATGATAGTGAAACTACTAAACTTTGA", "aa": "MKLKGFLAVGVSVFGFSGLLMACSVVSQFDQVDDGKIKLASSLTSKRAAEALETVVKKYNDTKDPGDYPIEIVQIAGGYDGGKKDVQTKVSTKDKNNFYNLILNYPEIVSTLSRSKMALNFDGINVDKLHPNFLSFNSRIGGIRDDGIYAIPISMSTDLMVINGPVLHYILNSARKEGTPTSTTVQATVSSRSAEKKGTLEIANDSETTKL", "aa_hexdigest": "2b46ba61fc8f1c3f2c249965a0a20b2e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22850.80850000001, "isoelectric_point": 8.499463462829592 }, "id": "KMADJIBOEG_593", "locus": "GCA000292445_00570" }, { "type": "cds", "sequence": "contig_1", "start": 311177, "stop": 312322, "strand": "+", "frame": 2, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0005886", "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75280", "UniRef:UniRef90_P47502" ], "nt": "TTGTTTGTTAACCGTGGTGGTTCCTATTCATCCAACTTTGAAAAATTTCACCAATTGGCATTCTCAGTATCTTCTTCTGGAGGATACAGTTATTACTTTGCTAAAGATAATGCTAAGCGCTTAAAGTTTAGTAATTATGCTATTGAATATCCTAGTTTTACCCAAACAATTCAAGCTCCTAATTCTTCAGAAACAGAAAGTAATTTACTTGGTACTTTTAAATTAAGTGAAAAAGATATCAATCTATATAAAGGTTCAATTCCTAGTGGAAAACAACAAGGAGTTGATGCTATCTTAATTAGTAACCCAAACTTAATTAATATTCTTGAACAAGCAAAACAAAAAAACACTGCACAAGGAAGTGAATCAACCACTAACAAGATAATAGGTTATACCGCCACTGCAAATGTTAATGTTGATAATCAAAACATCTTTTCTGTTAGCAAACTTAACAACGAACAGTTTCAAAGAAAAATCATTGTTAATGCCACTGAAGAAACACTTGATCAATCCCAAACCTTACAGAGCAATGAATCAATTGTTTTACCAATGCCTGGTAAATACAAATCAACTGATAAAAATAAAGTAATGATCACCCAAGGTCCTAACTTAATAGGCATCCATGCAAATGAAAAAGAAAATATTGAAACTAAAAAATTTGTTAATTGGTTTTTAAATCAAAGTATTACAGATTGGAATAGTAATAATCAGCAAAAAAATAGTGATCAAACAACAAAAACTGCTGCTGAATATTTCACTGATCAAGCTTCTTACATCCTTCCTTTAAAGGAAAAATTTAACAAAAGTTCAGATTTAGAATTGAAAGGCAGTAGTAGTTCTTCTAATTTAACAACCAGTAGTGCTAGCGCCTCTTTGTTAATAAGTAATAATAGTTCAACTGCAAGTTCTCCTGCTCCTAAAAAAACAAACAATAATTCTAATACCTTTACAGCTAAAGCACTAGAATTATTCCAACAAGCTGCTAACAATGAAATTATTCCCTTTAGTGATCCAAGTGACTTTAGGAACGGCACATTCCGTAATAATATAAGCAGTAGTTTTAATGCTGCGGTGAATTCTAAGGTTAGTTTTAATCAATTTGTCCAAAACTTTATTAATAGTTTAGGATCTGGATTTAGAAGATAA", "aa": "MFVNRGGSYSSNFEKFHQLAFSVSSSGGYSYYFAKDNAKRLKFSNYAIEYPSFTQTIQAPNSSETESNLLGTFKLSEKDINLYKGSIPSGKQQGVDAILISNPNLINILEQAKQKNTAQGSESTTNKIIGYTATANVNVDNQNIFSVSKLNNEQFQRKIIVNATEETLDQSQTLQSNESIVLPMPGKYKSTDKNKVMITQGPNLIGIHANEKENIETKKFVNWFLNQSITDWNSNNQQKNSDQTTKTAAEYFTDQASYILPLKEKFNKSSDLELKGSSSSSNLTTSSASASLLISNNSSTASSPAPKKTNNNSNTFTAKALELFQQAANNEIIPFSDPSDFRNGTFRNNISSSFNAAVNSKVSFNQFVQNFINSLGSGFRR", "aa_hexdigest": "82fd3662015734294c7f86db281df382", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 310877, "stop": 312322, "observations": { "mutation": [ 310880 ], "stop": [ 310880 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47502", "query_cov": 1.0, "subject_cov": 0.4980392156862745, "identity": 0.995, "score": 645.0, "evalue": 3.36e-226, "gene_start": 1, "gene_end": 381, "reference_start": 385, "reference_end": 765, "reference_sequence": "MKLKGFLAVGVSVFGFSGLLMACSVVSQFDQVDDGKIKLASSLTSKRAAEALETVVKKYNDTKDPGDYPIEIVQIAGGYDGGKKDVQTKVSTKDKNNFYNLILNYPEIVSTLSRSKMALNFDGVNVDKLHPNFLSFNSRIGGIRDDGIYAIPISMSTDLMVINGPVLHYILNSARKEGTPTSTTVQATVSSRSAEKKGTLEIANDSETTKLWQNIQTTAQNNSNETTKEQKQVKRSSSSSSTTSTTGETKDTTKSDNKIKEFWGEYQEVDGGLKNFTFKASIFENWNETLDFATRIANSFPEKVKNITNKTGLDLQGVLGVDSSSNALYAAVFAAGQANYDNFFFNIDKRTGYADYSNFLNKDSSYQNLESVYNDFYKLIQANGLFVNRGGSYSSNFEKFHQLAFSVSSSGGYSYYFAKDNAKRLKFSNYAIEYPSFTQTIQAPNSSETESNLLGTFKLSEKDINLYKGSIPSGKQQGVDAILISNPNLINILEQAKQKNTAQGSESTTNKIIGYTTTANVNVDNQNIFSVSKLNNEQFQRKIIVNATEETLDQSQTLQSNESIVLPMPGKYKSTDKNKVMITQGPNLIGIHANEKENIETKKFVNWFLNQSITDWNSNNQQKNSDQTTKTAAEYFTDQASYILPLKEKFNKSSDLELKGSSSSSNLTTSSASASLLISNNSSTASSPAPKKTNNNSNTFTAKALELFQQAANNEIIPFSDPSDFRNGTFRNNISSSFNAAVNSKVSFNQFVQNFINSLGSGFRR" }, "paralog": false, "identity": 0.9958419958419958, "score": 865.0, "evalue": 2.36e-315, "description": "Internal stop codon at 310880. Nonsense mutation around 310880.", "psc": { "uniref90_id": "UniRef90_P47502", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75280", "go_ids": [ "GO:0005886", "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47502", "UniRef:UniRef50_P75280", "GO:0005886", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75280", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75280" ], "product": "Uncharacterized lipoprotein MPN_506" } }, "id": "KMADJIBOEG_594", "locus": "GCA000292445_00571" }, { "type": "cds", "sequence": "contig_1", "start": 312351, "stop": 312608, "strand": "+", "frame": 3, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED94071", "UniRef:UniRef90_UPI001ED94071" ], "nt": "GTGTTACTAGTGCCTAAGGTGGTAGTGGAGTACCACCAACTTGATAAGGTTGTCAAAGAGAGTTTGGAAGTGGAAGCAACTTCCAACTTTGATCCAACTAAAAGGTTGCAAAAAGAGAGTCCAAAAAAGGATTCAAGTAAACAAGCGGAGAAACTCACCGAAACAACTTCATCGATGAGTAGTGGTGGGGCTACATCCACTCGAGCAAGGCCCTCAAGGTTGAGGTGGAAAGGGGGAGTCAAAGTGATTCACTTTTAA", "aa": "MLLVPKVVVEYHQLDKVVKESLEVEATSNFDPTKRLQKESPKKDSSKQAEKLTETTSSMSSGGATSTRARPSRLRWKGGVKVIHF", "aa_hexdigest": "d35fec9798f59c7374d309d40aee4844", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001ED94071", "query_cov": 0.8941176470588236, "subject_cov": 0.987012987012987, "identity": 1.0, "score": 142.0, "evalue": 5.68e-43, "valid": true, "product": "adhesin", "uniref50_id": "UniRef50_UPI001ED94071" }, "pscc": { "uniref50_id": "UniRef50_UPI001ED94071", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED94071" ], "product": "adhesin" }, "genes": [], "id": "KMADJIBOEG_595", "locus": "GCA000292445_00572" }, { "type": "cds", "sequence": "contig_1", "start": 313009, "stop": 313407, "strand": "+", "frame": 1, "gene": null, "product": "Random genomic clone esh10, partial cds", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49206" ], "nt": "GTGTTGCCTAGTAGTAGTAGCAACACGTTTAATCCCAATTCAGATGATAATAAAGTCACACCATCAGGTGGCTCCCCCAAAACAACCACCTATCCTTTTTTACCCAACTCCATCAGTCCCACCAGTGACTGGATCAATGCATTGACTTTCACTAATAAGAACAATCCACAACGAAATCAGTTGTTGCTCAGAAGCTTACTAGGAACTATTCCGGTATTGATCAATAAGAGTGGAACGGGAGATGAGTTTAACCATACGAGTGACCAGAAGTGGGATAAAACTGAGACAAATGAGGGCAACCTCCCGGGGTTTGGGGAGGTGAATGGTGCTTCTTATAAGATTTTTACTTATTTAATAATTAAAAAAAGTGTTAGGTTTTTTAGTTTTTTATCTATTTAA", "aa": "MLPSSSSNTFNPNSDDNKVTPSGGSPKTTTYPFLPNSISPTSDWINALTFTNKNNPQRNQLLLRSLLGTIPVLINKSGTGDEFNHTSDQKWDKTETNEGNLPGFGEVNGASYKIFTYLIIKKSVRFFSFLSI", "aa_hexdigest": "392de227e1fc6529b8b06b7541672740", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030D20171", "query_cov": 0.8787878787878788, "subject_cov": 0.9830508474576272, "identity": 0.845, "score": 184.0, "evalue": 6.46e-58, "valid": false, "product": "adhesin", "uniref50_id": "UniRef50_Q49206" }, "pscc": { "uniref50_id": "UniRef50_Q49206", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49206" ], "product": "Random genomic clone esh10, partial cds" }, "genes": [], "id": "KMADJIBOEG_596", "locus": "GCA000292445_00573" }, { "type": "cds", "sequence": "contig_1", "start": 313860, "stop": 314036, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13", "UniRef:UniRef90_UPI0030D43D23" ], "nt": "GTGGTGAAAAAGTATCAGTTAGATCTACCAAATGTTACCAATGAGATGAACCAAGTGTTGCAAGAATTGATCCTTGAACAACCTTTGACTAAGTATACCTTAAACAGTAGTTTGGCCAAACAAAAGGGCAAAAGCCAGATAGAGGTGCATCTGGGTAGTGGGCAAGCAAATAATTGA", "aa": "MVKKYQLDLPNVTNEMNQVLQELILEQPLTKYTLNSSLAKQKGKSQIEVHLGSGQANN", "aa_hexdigest": "9e9bcd42e3e74d8a183db7db44fa4491", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 313737, "stop": 314095, "observations": { "deletion": [ 314052 ], "mutation": [ 313854, 314034 ], "stop": [ 313854, 314034 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0030D43D23", "query_cov": 0.9655172413793104, "subject_cov": 0.4628099173553719, "identity": 0.875, "score": 99.0, "evalue": 1.57e-25, "gene_start": 1, "gene_end": 56, "reference_start": 42, "reference_end": 97, "reference_sequence": "MKLTYSSSGSNTNTISFENNANKPSYVVEFTNSTNVGIKWRVVKKYQLDVPNVSSDMNQVLQELILEQPLTKYTLNSSLAKQKGKSKIEVHLGSGRATQWTSRTQLHWPKQQSQPKCINRI" }, "paralog": false, "identity": 0.7394957983193278, "score": 167.0, "evalue": 3.92e-55, "description": "Internal stop codon at 313854, 314034. Frameshift due to deletion around 314052. Nonsense mutation around 313854, 314034.", "psc": { "uniref90_id": "UniRef90_UPI0030D43D23", "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0030D43D23", "UniRef:UniRef50_UPI00027B3D13" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6526.408800000001, "isoelectric_point": 8.05663013458252 }, "id": "KMADJIBOEG_597", "locus": "GCA000292445_00574" }, { "type": "cds", "sequence": "contig_1", "start": 314473, "stop": 314742, "strand": "+", "frame": 1, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "RefSeq:WP_318023378.1", "SO:0001217", "UniParc:UPI00031FAA91", "UniRef:UniRef100_UPI00031FAA91", "UniRef:UniRef50_UPI0030D266AC", "UniRef:UniRef90_UPI00031FAA91" ], "nt": "ATGACCAACTTGTTAAAGTTACTGACAACTAAAAATGTGAAAGCAAAGTTGGGGAAGGGAACAGCTTCTTCGCAGGGAAATAGTGGAGGGGGTGTTAGTCAAACGATTAATACCATCGCTACTACGGGGAATATTAGTGGCAATGGAACCATTCAAACGGCTTATCCGGTGAAAAAAGATGAAGCTTCAAATGTAGCGATCAATTCCTTGATTAACGCTACGCCCTTGAATAGTTATGGGGATTTAAATAATGCTAGTTTTTCTAAATAA", "aa": "MTNLLKLLTTKNVKAKLGKGTASSQGNSGGGVSQTINTIATTGNISGNGTIQTAYPVKKDEASNVAINSLINATPLNSYGDLNNASFSK", "aa_hexdigest": "89e57905b922b89337bc75730f0ea3f7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00031FAA91", "ncbi_nrp_id": "WP_318023378.1", "uniref100_id": "UniRef100_UPI00031FAA91", "db_xrefs": [ "SO:0001217", "UniParc:UPI00031FAA91", "RefSeq:WP_318023378.1", "UniRef:UniRef100_UPI00031FAA91" ] }, "ips": { "uniref100_id": "UniRef100_UPI00031FAA91", "uniref90_id": "UniRef90_UPI00031FAA91" }, "psc": { "uniref90_id": "UniRef90_UPI00031FAA91", "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI0030D266AC" }, "pscc": { "uniref50_id": "UniRef50_UPI0030D266AC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030D266AC" ], "product": "MgpC family cytadherence protein" }, "genes": [ "mgpC" ], "id": "KMADJIBOEG_598", "locus": "GCA000292445_00575" }, { "type": "tRNA", "sequence": "contig_1", "start": 314811, "stop": 314887, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(tcg)", "amino_acid": "Arg", "anti_codon": "tcg", "score": 73.0, "nt": "GCGCCCATAGCTCAATCGGATAGAGTGTCTGGCTTCGGACCAGAAGGTTATGGGTTCAAGTCCTATTGGGCGCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 314870, 314872 ], "id": "KMADJIBOEG_31", "locus": "GCA000292445_00576" }, { "type": "cds", "sequence": "contig_1", "start": 314977, "stop": 315207, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MPN_377", "db_xrefs": [ "RefSeq:WP_009885892.1", "SO:0001217", "UniParc:UPI0000557E10", "UniRef:UniRef100_UPI0000557E10", "UniRef:UniRef50_Q9EXD3", "UniRef:UniRef90_UPI0000557E10" ], "nt": "ATGGCAAAAGATAAAAAAAATAAGGTTGATGGAACAGAGCAATCAGTTGATCTATTTGAACGTACAAAACTTGAAGATACACAAGTTTTAAATGAAGTTGAACTTGATGATATTAAAAAGATAACAGAGCTTAGAAAAGAACTTGAACATACTTTTGAACCACAAACAAGAATGCAAATTAAGCGTGAAATTAAAGAAATAGAACGCAAAATGAAACGTTCTAGTCGCTAA", "aa": "MAKDKKNKVDGTEQSVDLFERTKLEDTQVLNEVELDDIKKITELRKELEHTFEPQTRMQIKREIKEIERKMKRSSR", "aa_hexdigest": "216d36a3184e8aa2b7fdf2f737d21396", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000557E10", "ncbi_nrp_id": "WP_009885892.1", "uniref100_id": "UniRef100_UPI0000557E10", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000557E10", "RefSeq:WP_009885892.1", "UniRef:UniRef100_UPI0000557E10" ] }, "ips": { "uniref100_id": "UniRef100_UPI0000557E10", "uniref90_id": "UniRef90_UPI0000557E10" }, "psc": { "uniref90_id": "UniRef90_UPI0000557E10", "uniref50_id": "UniRef50_Q9EXD3" }, "pscc": { "uniref50_id": "UniRef50_Q9EXD3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9EXD3" ], "product": "Uncharacterized protein MPN_377" }, "genes": [], "id": "KMADJIBOEG_599", "locus": "GCA000292445_00577" }, { "type": "cds", "sequence": "contig_1", "start": 315211, "stop": 315573, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGTTAATTTACATACAAATTCATACTATAACTTTCTCAATTCTGCCCTTTCTCCTAAAAAGCTAGTTAATCTAGCAATTAATGATCAGCAAAAAGCTGTTGCTATTACAGATCCTAATCTTTTTGGCGCTGTTGAATTTTTTATAACTTGTAAGCAAAATAATATTAAACCAATTATTGGTTTAAACTTAACTGTTGAATACCAAAAAAATGATGTTAAGTTATTACTAATTGCTAAATCAAATAAAGGCTTTCAAACGTTGAACAAAATAGCATTAATTCAACAAAAACTTGAAATTAATTCTTTAGTTGATCAACTAACAGATATTGCAGTAATTATCTGTTCTTTAACAACATGA", "aa": "MFVNLHTNSYYNFLNSALSPKKLVNLAINDQQKAVAITDPNLFGAVEFFITCKQNNIKPIIGLNLTVEYQKNDVKLLLIAKSNKGFQTLNKIALIQQKLEINSLVDQLTDIAVIICSLTT", "aa_hexdigest": "a11942cdc52a670fb4f4b451b90467ff", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13410.536799999982, "isoelectric_point": 8.928629493713384 }, "id": "KMADJIBOEG_600", "locus": "GCA000292445_00578" }, { "type": "cds", "sequence": "contig_1", "start": 315706, "stop": 316818, "strand": "+", "frame": 1, "gene": "dnaE", "product": "DNA polymerase III subunit alpha", "db_xrefs": [ "COG:COG0587", "COG:L", "EC:2.7.7.7", "GO:0003887", "GO:0005737", "GO:0006260", "GO:0008408", "SO:0001217", "UniRef:UniRef50_Q49405", "UniRef:UniRef90_Q49405" ], "nt": "ATGAAACAAAACCAAACGGGAAAAATAACTACATTTGATTGGGATTTTAAACAAAAATTAAATCAAATTTCAATTAATGAAAATTTAAAAGTAAAGAGTGAAATTCAACCTTTTTTAGATCAAAAAACTGCACAACAATTATTCAGTGAAACAGAACTTAATAATCTGAATGATCTAGTTAATAGATGTGAATTAGATTTGGAGCACCTAAAAGCTGCTTCACTTTCTTTAACTGATAATGATGCAGCAGTTTTAGAAAGTTTGTGCCAAACCAATTTAAAACAGTTTTTAGATAAAAATCAAGATCTAAATAAAAAAGCCTATCGGCTACGTTTAGAGAAGGAATTAAATGTTATCAATAAACTTAATTTTGCTAGCTATTTTTTAGTTGTCAATGATCTTGTTAATTATGCTTTTAAAAAGGACATCTTAATTGGTTCTGGTAGAGGTTCTGCAGTAGGATCATTAGTGGCTTTTTTATTAAACATTACCAAGATAGACCCAGTCCAACACCAGCTTATTTTCGAACGTTTTATCTCAACCCACCGTCAAGATCTACCTGATATTGATATTGATATCATGGAGAATAAAAGAGCAGAAATGATAAATTATCTGTTTGAAAAATATGGCAAAGAAAACTGTGCACAAATTGTTACTTTTCAACGTTTTAAAACCCGTTCTGCTGTTAAAGAAGTTGCTAAATTATTTAATGATTATGGCATTAGTGACATGATCCTAGGAGTGTTACCTAAAGATCAAACTATAACATTCACTGAGCTTAAAGCTACTGAAGATAGTGCTTTACAACTTTGTTTACAACAGTTTGGTTTAATTGTTGAATTAGCACTAGCAATAGTTGATTTTCCAAGACAATCAAGTATCCATGCTTCAGGCATAGTTATCGCTTCAAATTCTTTGATTAAAACCATTCCCTTGTTACAGCTTGACAATAATCACTTTTTAACTCAAGTTTCAATGGAATGGTTAAGTTTTTTTAATCTCAATAAGTTTGATCTGCTTGGTTTAATTAACCTTACTATGATTAGCGATGTAATTACCCAAATTAAACCATCTAACCAGACCGTTAACCAGTTTTTAAATACCATTTCTTGA", "aa": "MKQNQTGKITTFDWDFKQKLNQISINENLKVKSEIQPFLDQKTAQQLFSETELNNLNDLVNRCELDLEHLKAASLSLTDNDAAVLESLCQTNLKQFLDKNQDLNKKAYRLRLEKELNVINKLNFASYFLVVNDLVNYAFKKDILIGSGRGSAVGSLVAFLLNITKIDPVQHQLIFERFISTHRQDLPDIDIDIMENKRAEMINYLFEKYGKENCAQIVTFQRFKTRSAVKEVAKLFNDYGISDMILGVLPKDQTITFTELKATEDSALQLCLQQFGLIVELALAIVDFPRQSSIHASGIVIASNSLIKTIPLLQLDNNHFLTQVSMEWLSFFNLNKFDLLGLINLTMISDVITQIKPSNQTVNQFLNTIS", "aa_hexdigest": "721755867c14d62c601eca0a50c5a11d", "start_type": "ATG", "rbs_motif": null, "genes": [ "dnaE" ], "truncated": "both", "pseudogene": { "start": 315406, "stop": 317121, "observations": { "mutation": [ 315571, 316816 ], "stop": [ 315571, 316816 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49405", "query_cov": 1.0, "subject_cov": 0.4233409610983982, "identity": 0.992, "score": 707.0, "evalue": 4.41e-249, "gene_start": 1, "gene_end": 370, "reference_start": 166, "reference_end": 535, "reference_sequence": "MFVNLHTNSYYNFLNSALSPKKLVNLAINDQQKAVAITDPNLFGAVEFFITCKQNNIKPIIGLNLTVEYQKNDVKLLLIAKSNKGFQTLNKIALIQQKLEINSLVDQLTDIAVIICSLTTWKSTYKDVYQAKGIEINQTPIAILANAVNCEKTNSDQVVLTVLKQMKQNQTGKITTFDWDLKQKLNQISINENLKVKSEIQPFLDQKTAQQLFSETELNNLNDLVNRCELDLEHLKAASLSLTDNDAAVLESLCQTNLKQFLDKNQDLNKKAYQLRLEKELNVINKLNFASYFLVVNDLVNYAFKKDILIGSGRGSAVGSLVAFLLNITKIDPVQHQLIFERFISTHRQDLPDIDIDIMENKRAEMINYLFEKYGKENCAQIVTFQRFKTRSAVKEVAKLFNDYGISDMILGVLPKDQTITFTDLKATEDSALQLCLQQFGLIVELALAIVDFPRQSSIHASGIVIASNSLIKTIPLLQLDNNHFLTQVSMEWLSFFNLNKFDLLGLINLTMISDVITQIKPSNQTVNQFLNTISWTDQNTFINLVNEDTLGIFQLESFGMKKLLVQIKPKTINQLAIVLALYRPGAQDNINLFINRLHNGYDQSDIDPRILPIVKNTYGVLIFQEQIINIVKVVANYSLEEADSFRRAISKKDVKLIQKNKRNFFERAVQNNFDLKTTTKIFSYIERFANYGFNLSHALGYALLSYWTAWLKTNYPVYFYLWLLNHFQSSKDKQKLIIRTLEKSGIEIYPPLLNKAQPNSVIENKKIYLGLNLIKGINDRYIQNLQKVQHLIQTQNNLQLTDVVSWCLDKTIGDIPLKDLLLLKTMGCFDFFEYTYDFNDAKDFWIKSDHLLFTRMPLEKKDSNFWIKQFFTN" }, "paralog": false, "identity": 0.9912434325744308, "score": 1093.0, "evalue": 0.0, "description": "Internal stop codon at 315571, 316816. Nonsense mutation around 315571, 316816.", "psc": { "uniref90_id": "UniRef90_Q49405", "gene": "dnaE", "product": "DNA polymerase III subunit alpha", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_Q49405", "cog_id": "COG0587", "cog_category": "L", "go_ids": [ "GO:0005737", "GO:0008408", "GO:0003887", "GO:0006260" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49405", "EC:2.7.7.7", "UniRef:UniRef50_Q49405", "COG:COG0587", "COG:L", "GO:0005737", "GO:0008408", "GO:0003887", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_Q49405", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49405" ], "product": "DNA polymerase III subunit alpha" } }, "id": "KMADJIBOEG_601", "locus": "GCA000292445_00579" }, { "type": "cds", "sequence": "contig_1", "start": 316891, "stop": 317334, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17657.6" ], "nt": "ATGAAAAAATTACTGGTTCAGATTAAACCTAAAACCATTAATCAACTAGCAATTGTTCTAGCGCTTTACAGACCAGGTGCACAGGATAACATTAACCTTTTTATTAACCGTTTGCACAATGGTTATGATCAATCTGACATTGATCCTAGGATTTTACCCATTGTGAAAAATACCTATGGAGTTTTAATTTTTCAAGAGCAGATCATTAACATCGTTAAAGTTGTGGCTAACTACTCTTTAGAAGAAGCAGATAGCTTCCGTAGAGCCATTTCTAAAAAGGATGTTAAATTGATCCAAAAAAATAAGCGTAACTTCTTTGAAAGAGCAGTTCAAAATAACTTTGATTTAAAGACTACTACCAAAATTTTTAGCTACATAGAACGCTTTGCTAACTATGGGTTTAACCTTTCTCATGCGTTGGGTTATGCACTGCTTTCATACTGA", "aa": "MKKLLVQIKPKTINQLAIVLALYRPGAQDNINLFINRLHNGYDQSDIDPRILPIVKNTYGVLIFQEQIINIVKVVANYSLEEADSFRRAISKKDVKLIQKNKRNFFERAVQNNFDLKTTTKIFSYIERFANYGFNLSHALGYALLSY", "aa_hexdigest": "a1a9a9b13fbce59c5aa0dabbc9c84b39", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17657.6", "name": "DNA_pol3_finger", "length": 115, "aa_cov": 0.7687074829931972, "hmm_cov": 1.0, "evalue": 1.2422458809143162e-36, "score": 120.6449966430664, "start": 1, "stop": 113 } ], "seq_stats": { "molecular_weight": 17055.596099999988, "isoelectric_point": 9.785091972351072 }, "id": "KMADJIBOEG_602", "locus": "GCA000292445_00580" }, { "type": "cds", "sequence": "contig_1", "start": 317575, "stop": 317748, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAACATTTAATTCAAACTCAAAATAACTTACAACTAACTGATGTAGTAAGTTGGTGTTTGGATAAAACCATTGGTGATATCCCTTTAAAAGATTTACTTTTATTAAAAACTATGGGCTGTTTTGATTTTTTTGAATACACTTATGACTTTAATGATGCAAAGGATTTTTGA", "aa": "MQHLIQTQNNLQLTDVVSWCLDKTIGDIPLKDLLLLKTMGCFDFFEYTYDFNDAKDF", "aa_hexdigest": "69c2fb9a3f3b43dc08c1018e39d14ff5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6720.630399999998, "isoelectric_point": 4.222591590881348 }, "id": "KMADJIBOEG_603", "locus": "GCA000292445_00581" }, { "type": "cds", "sequence": "contig_1", "start": 317962, "stop": 318717, "strand": "+", "frame": 1, "gene": "polA", "product": "5'-3' exonuclease", "db_xrefs": [ "COG:COG0258", "COG:L", "EC:3.1.11.-", "GO:0003677", "GO:0008409", "GO:0017108", "GO:0033567", "SO:0001217", "UniRef:UniRef50_P75403", "UniRef:UniRef90_Q49406" ], "nt": "ATGTTACTAATGTGTTGGAATTTAATTAAAGCCAATGTTTATCAATATGGAATTGTAAGTTTTGACACTAAAGCGCCAACTTTCCGTGATCAAATCTATGAAGGATATAAACAAAAAAGGGTTAAAACTCCAGTTGAACTTTTAGTACAAATTCCTCTAATTAAACAAGCGCTTGTTTATTTAGGGTTTTTAGTTTGTGAAAAAGATGGCTTTGAAGCAGATGATTTGATTGGTAGTTATGCCAATTTATTTACAAAGCAAGAAATAACAGTTGATATTTACAGTTCAGATCGAGATATGTTGCAATTAGTAAACGCCTTTACTAATGTGTTTCTCTGTGTTAAAGGTACAAAGGAGATGGTTATGTACAACAATGAAAATTTCAAATCACTTTTTTATGGTTTGGCGCCCTATCAAGTTGTTGAATATAAGGGGTTAGTTGGTGATAACAGCGATAATTTAGCAGGGATTAAAGGGATAGGTCCCATCAAAGGGATAGAATTACTCCAACAATATGGAACCATTGATAACATCTACACTAACTTCAATAATCTCCCCAACCAACTTCAAAAACTTTTAAATAACCAAAAGGAAATAGCTAAAACCTTTAGTTTTCTAGCTAAAATTAAAACTGATATTGAACTTGATCAAAACATAGATCTTACTGGTTTAAAACCAATCCAAAAACAAGCGTTAATTCAACTTCTAAGTGAAAACAAAATTAATACTTTAGTTGAAAAATTTTCAAAAATATAA", "aa": "MLLMCWNLIKANVYQYGIVSFDTKAPTFRDQIYEGYKQKRVKTPVELLVQIPLIKQALVYLGFLVCEKDGFEADDLIGSYANLFTKQEITVDIYSSDRDMLQLVNAFTNVFLCVKGTKEMVMYNNENFKSLFYGLAPYQVVEYKGLVGDNSDNLAGIKGIGPIKGIELLQQYGTIDNIYTNFNNLPNQLQKLLNNQKEIAKTFSFLAKIKTDIELDQNIDLTGLKPIQKQALIQLLSENKINTLVEKFSKI", "aa_hexdigest": "e90c38a59260d249dc7c3810d187c4bb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q49406", "query_cov": 1.0, "subject_cov": 0.8625429553264605, "identity": 0.996, "score": 494.0, "evalue": 3.38e-176, "valid": true, "gene": "polA", "product": "5'-3' exonuclease", "ec_ids": [ "3.1.11.-" ], "uniref50_id": "UniRef50_P75403", "cog_id": "COG0258", "cog_category": "L", "go_ids": [ "GO:0008409", "GO:0017108", "GO:0003677", "GO:0033567" ] }, "pscc": { "uniref50_id": "UniRef50_P75403", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75403" ], "product": "5'-3' exonuclease" }, "genes": [ "polA" ], "id": "KMADJIBOEG_604", "locus": "GCA000292445_00582" }, { "type": "cds", "sequence": "contig_1", "start": 318717, "stop": 319571, "strand": "+", "frame": 3, "gene": "mutM", "product": "DNA-formamidopyrimidine glycosylase", "db_xrefs": [ "COG:COG0266", "COG:L", "EC:3.2.2.23", "GO:0003684", "GO:0003906", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0008270", "GO:0008534", "GO:0034039", "GO:0046872", "GO:0140078", "KEGG:K10563", "RefSeq:WP_010869400.1", "SO:0001217", "UniParc:UPI000012AB8B", "UniRef:UniRef100_P55825", "UniRef:UniRef50_P55825", "UniRef:UniRef90_P55825" ], "nt": "ATGCCTGAACTTCCTGAAGTAACTACTGTTATTAATGAACTTAAAGAAACTGTTTTAAATAAACCTTTAGATCAAGTTCAAGTTAACCTAAGAAAGGTTTTGAAAAATATTGATCCTCAATTGCTGAATAAACAATTAAAAAATCAGTTTTTTACTGATATTAAGCGTAAGGGTAAATATATCATTTTTCTTTTAAGTAATGGTTTGTATTTAGTTTCGCATTTACGTATGGAAGGTAAATACTTTTTTGAAGAAAGAGGTAGTAAATTTAATCAAAAGCATGTTTTAGTAGAATTTCATTTTGATGATGGTAGTCAACTCAATTATCATGACACCAGACAATTTGGAACGTTCCATTTGTATGAAAAGTTAGAACAAGCAGCACAATTAAATAAACTTGCATTTGATCCTCTAGAAGCTGGTTTTGACTATAGGAAAATCTTCCAAAAAGCACAAAATTCAAAACGTAAAGTTAAAACTTTTATTTTAGACCAAACAGTGATTAGTGGAATTGGCAATATTTATGCAGATGAAATCTTATTTGCAAGCAAAATTAATCCTGAAACAATGGTTGATCAACTAACAATTAAAGAGATAGAGATTTTATGTAAAAATGCTACCAAAATTTTAGCTAAAGCAATAGTTATGAAAGGTACTACCATCAGCAGCTTTAGTTTTAAAAAAGATCATACTGGAGGCTATCAAAACTTTTTAAAAGTTCACACTAAAAAAGATCAACCTTGCTCAGTTTGTAACCAATTAATTGTTAAAAAGAAGATTAATGGAAGGGGGAGCTATTTTTGTTTAAACTGTCAAAAAATCACAACCAAAGTTTCTACAAAACTCAATCCATAA", "aa": "MPELPEVTTVINELKETVLNKPLDQVQVNLRKVLKNIDPQLLNKQLKNQFFTDIKRKGKYIIFLLSNGLYLVSHLRMEGKYFFEERGSKFNQKHVLVEFHFDDGSQLNYHDTRQFGTFHLYEKLEQAAQLNKLAFDPLEAGFDYRKIFQKAQNSKRKVKTFILDQTVISGIGNIYADEILFASKINPETMVDQLTIKEIEILCKNATKILAKAIVMKGTTISSFSFKKDHTGGYQNFLKVHTKKDQPCSVCNQLIVKKKINGRGSYFCLNCQKITTKVSTKLNP", "aa_hexdigest": "bbd5555ec735efd141f97aacfaa496b8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012AB8B", "ncbi_nrp_id": "WP_010869400.1", "uniref100_id": "UniRef100_P55825", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012AB8B", "RefSeq:WP_010869400.1", "UniRef:UniRef100_P55825" ] }, "ips": { "uniref100_id": "UniRef100_P55825", "uniref90_id": "UniRef90_P55825" }, "psc": { "uniref90_id": "UniRef90_P55825", "gene": "mutM", "product": "DNA-formamidopyrimidine glycosylase", "ec_ids": [ "3.2.2.23" ], "uniref50_id": "UniRef50_P55825", "cog_id": "COG0266", "cog_category": "L", "kegg_orthology_id": "K10563", "go_ids": [ "GO:0003684", "GO:0003906", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0008270", "GO:0008534", "GO:0034039", "GO:0046872", "GO:0140078" ] }, "pscc": { "uniref50_id": "UniRef50_P55825", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P55825" ], "product": "Formamidopyrimidine-DNA glycosylase" }, "genes": [ "mutM" ], "id": "KMADJIBOEG_605", "locus": "GCA000292445_00583" }, { "type": "cds", "sequence": "contig_1", "start": 319918, "stop": 320064, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTACCATTACTTTAGATGGGGCTATTATCTTTTATCAAAACGAAATCTTAAGTCAAACTTATTTAGATGATAGATTTATTGAAGGGATTAATAACATAGTAAAGCGCTTTCCTGAAGCTGCTTATAAACTTAACAGTGGTTGA", "aa": "MPTITLDGAIIFYQNEILSQTYLDDRFIEGINNIVKRFPEAAYKLNSG", "aa_hexdigest": "f20adc33eaa4cc4f8bdb9c3ebc48e9ec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5494.1902, "isoelectric_point": 4.593750190734863 }, "id": "KMADJIBOEG_606", "locus": "GCA000292445_00584" }, { "type": "cds", "sequence": "contig_1", "start": 320242, "stop": 320439, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGCAAAAGGAAATAATTGAACTAATTAGTGTTAGTGATGTAAATGCCAAAATCTATGAGCACTCTATGTACATTATTAAAAATGGTGTTTCTAAAGCTAGTGCATTGCAAAGCCTTAACCAGTTTGCAATTCCAATAACAAAAGATAACACTATTGTTTGTGGGGATGGAGATAATGACATTGAAATGATGCAGTGA", "aa": "MQKEIIELISVSDVNAKIYEHSMYIIKNGVSKASALQSLNQFAIPITKDNTIVCGDGDNDIEMMQ", "aa_hexdigest": "d47c9668724ae7cabc2cd0138eac8d98", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 50, "aa_cov": 0.7692307692307693, "hmm_cov": 0.98, "evalue": 3.39558039566233e-11, "score": 38.46260070800781, "start": 16, "stop": 65 } ], "seq_stats": { "molecular_weight": 7217.211100000002, "isoelectric_point": 4.5550428390502935 }, "id": "KMADJIBOEG_607", "locus": "GCA000292445_00585" }, { "type": "cds", "sequence": "contig_1", "start": 320556, "stop": 320834, "strand": "+", "frame": 3, "gene": "coaE", "product": "dephospho-CoA kinase", "db_xrefs": [ "COG:COG0237", "COG:H", "EC:2.7.1.24", "GO:0004140", "GO:0005524", "GO:0005737", "GO:0015937", "KEGG:K00859", "SO:0001217", "UniRef:UniRef50_P47506", "UniRef:UniRef90_P47506" ], "nt": "ATGTTAATTGCAATCGTAGGTAAACCAGGTGTTGGTAAAACCAGTCTATTGCAATATCTCAAAGATAACTATCACTTTTCAGTTTTTTATGCAGATAGTTTTATCCATGAACAGTACCAAAAAAACAATCCAGGTTATCAATTAATCATGGATCATTTTGGCAAAGAGTTTGTCAATCAAACTGAAGTTGATCGTAAAAAACTAGCAAACTATGTTTTTAGTGATGATAAGTTAATCGAAAAACTTTCACTAGTAACAAAACCGCTGTTAATAGCGTGA", "aa": "MLIAIVGKPGVGKTSLLQYLKDNYHFSVFYADSFIHEQYQKNNPGYQLIMDHFGKEFVNQTEVDRKKLANYVFSDDKLIEKLSLVTKPLLIA", "aa_hexdigest": "451dec50d2b01e479ee19b450e831152", "start_type": "ATG", "rbs_motif": null, "genes": [ "coaE" ], "truncated": "3-prime", "pseudogene": { "start": 320556, "stop": 321137, "observations": { "mutation": [ 320832, 320904 ], "stop": [ 320832, 320904 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47506", "query_cov": 1.0, "subject_cov": 0.46464646464646464, "identity": 1.0, "score": 185.0, "evalue": 5.69e-58, "gene_start": 1, "gene_end": 92, "reference_start": 1, "reference_end": 92, "reference_sequence": "MLIAIVGKPGVGKTSLLQYLKDNYHFSVFYADSFIHEQYQKNNPGYQLIMDHFGKEFVNQTEVDRKKLANYVFSDDKLIEKLSLVTKPLLIAWIKSLKTQFQKKLALIEIAVMLNYWNEYRSLFDYVIKLERDDQLVNLALQQRNSHKKVKDLIKEPNCKIDTIFNNDSIATAALKLIKLLETFLERNKCRCDCCHIQ" }, "paralog": false, "identity": 0.9896373056994818, "score": 377.0, "evalue": 4.33e-136, "description": "Internal stop codon at 320832, 320904. Nonsense mutation around 320832, 320904.", "psc": { "uniref90_id": "UniRef90_P47506", "gene": "coaE", "product": "dephospho-CoA kinase", "ec_ids": [ "2.7.1.24" ], "uniref50_id": "UniRef50_P47506", "cog_id": "COG0237", "cog_category": "H", "kegg_orthology_id": "K00859", "go_ids": [ "GO:0004140", "GO:0005524", "GO:0005737", "GO:0015937" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47506", "EC:2.7.1.24", "UniRef:UniRef50_P47506", "COG:COG0237", "COG:H", "KEGG:K00859", "GO:0004140", "GO:0005524", "GO:0005737", "GO:0015937" ] }, "pscc": { "uniref50_id": "UniRef50_P47506", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47506" ], "product": "Dephospho-CoA kinase" } }, "id": "KMADJIBOEG_608", "locus": "GCA000292445_00586" }, { "type": "cds", "sequence": "contig_1", "start": 320934, "stop": 321152, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTAAATTAGAAAGGGATGATCAGCTAGTTAACTTAGCTTTACAACAACGTAATAGTCATAAAAAAGTTAAGGATTTGATTAAAGAGCCTAATTGCAAAATAGATACAATTTTCAACAACGATTCGATTGCAACAGCTGCTTTAAAGCTAATTAAGTTGCTAGAAACTTTTTTAGAAAGAAATAAATGCCGTTGTGATTGTTGTCATATTCAGTAA", "aa": "MIKLERDDQLVNLALQQRNSHKKVKDLIKEPNCKIDTIFNNDSIATAALKLIKLLETFLERNKCRCDCCHIQ", "aa_hexdigest": "dff1722a4cb657f92f8c5d87688485c6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8396.792599999993, "isoelectric_point": 8.678105354309082 }, "id": "KMADJIBOEG_609", "locus": "GCA000292445_00587" }, { "type": "cds", "sequence": "contig_1", "start": 321088, "stop": 321924, "strand": "-", "frame": 1, "gene": "cof", "product": "Putative phosphatase MG265", "db_xrefs": [ "COG:COG0561", "COG:HR", "EC:3.1.3.-", "GO:0000287", "GO:0005829", "GO:0016791", "KEGG:K21064", "RefSeq:WP_009885896.1", "SO:0001217", "UniParc:UPI0000139535", "UniRef:UniRef100_P47507", "UniRef:UniRef50_P75399", "UniRef:UniRef90_P47507" ], "nt": "ATGGAACTGAAAAACATTATTTTTGACCTTGATGGTACCTTGCTTTCAAGCAACCAAATTCCATTAGAACAAACAGTTGAGTTTTTAAAGGATTTACAGAAAAAAGGGATTAGAATCACTTTTGCTAGTGGTAGAAGCCATATTTTAATTAGAAACACAGCTACCTTTATTACACCAAATCTACCTGTAATTTCTTCCAATGGTGCACTTGTTTATGATTTTGCTAGTGAAAAACCAGTTCATATCAAACCTATTGATAATAAAGTAATACCTGCAATTATGCAAATGTTGTTGGAATTTCAAGAAACATTTTATTTCTATACAGATAAAAAGGTTTTTGCTTTTACACATGAGCTTGATTCAGCTAAAATTCTTTCAACTAGAAGTCAAATAGTAGGAATTGATCTCATTGAAAATAACTACATAGTTAACAAGTTTGAAAAAGCTTTGGATTTTGATTTTAAGCAACATACTATTACAAAGATCTTACTGGTAACTAAAAACAGAGAAAAAGTTCCTTTTCTAGCAAAACAACTAGATCAAATTCAAGATATTAACTATGTGAGTTCAATGACATTTGCTCTTGATATCATGCAAAAAGATGTTAATAAGGCTTATGGATTGAAAGTATTAGTTGATAATTATAATCTTGATCCTGAAAAGACTATGGTCTTTGGTGATGCTGATAATGATGTTGAAATATTTCAAAGTGTTAAATGGCCAGTTGCTTTGGTTAATGGCACTGATTTAGCTAAAAAAAATGCTAAGTTTATTACTGAATATGACAACAATCACAACGGCATTTATTTCTTTCTAAAAAAGTTTCTAGCAACTTAA", "aa": "MELKNIIFDLDGTLLSSNQIPLEQTVEFLKDLQKKGIRITFASGRSHILIRNTATFITPNLPVISSNGALVYDFASEKPVHIKPIDNKVIPAIMQMLLEFQETFYFYTDKKVFAFTHELDSAKILSTRSQIVGIDLIENNYIVNKFEKALDFDFKQHTITKILLVTKNREKVPFLAKQLDQIQDINYVSSMTFALDIMQKDVNKAYGLKVLVDNYNLDPEKTMVFGDADNDVEIFQSVKWPVALVNGTDLAKKNAKFITEYDNNHNGIYFFLKKFLAT", "aa_hexdigest": "a8175bd90a9b8fe365110442e827d3a1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139535", "ncbi_nrp_id": "WP_009885896.1", "uniref100_id": "UniRef100_P47507", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139535", "RefSeq:WP_009885896.1", "UniRef:UniRef100_P47507" ] }, "ips": { "uniref100_id": "UniRef100_P47507", "uniref90_id": "UniRef90_P47507" }, "psc": { "uniref90_id": "UniRef90_P47507", "gene": "Cof", "product": "Putative phosphatase MG265", "ec_ids": [ "3.1.3.-" ], "uniref50_id": "UniRef50_P75399", "cog_id": "COG0561", "cog_category": "HR", "kegg_orthology_id": "K21064", "go_ids": [ "GO:0005829", "GO:0000287", "GO:0016791" ] }, "pscc": { "uniref50_id": "UniRef50_P75399", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75399" ], "product": "Putative phosphatase MPN_383" }, "genes": [ "cof" ], "id": "KMADJIBOEG_610", "locus": "GCA000292445_00588" }, { "type": "cds", "sequence": "contig_1", "start": 321943, "stop": 322464, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08264.19" ], "nt": "TTGGATCGAGTTTATAACTTCTTTTTTAATCATGCTGTTGTTACTGATCAAGTTAGTCAAGAGACAACCTTTGCTTACAATTTGTTTTTAAAAAACAGTTATTGTCATCTTGACAAACATGAACTAAATCTGGTGATTAGTGAAATGATGATCTTTTTAAACTTTCTCTATAAAACCAAAAAAATTAGCTTAAATTATGCAAAGGGATTTTTAACAGTACTGTCGTTTTTTGCGCCCTTTCTTGCTGAAGAATTGAATGAAAAATGTGGACTTGAACCATTTGTTGTTAAACAAGCGATTTCTTTAGTTGATTATCAACTTTTTGAGACTGCTAAAACTAAGGTTATTCTTTCAATTAATGGCAAATTTAAAGCAGCTAAAGAATTTACTAAAGGTAGTTTAGAGATAGATGTTTTAGAATCATTTAAACAGGATAAAGAGATAAATGACATTCTCAACCAACCGATTGAGAGGGTAGTTTATGTTCAGGATCGAATTATTAATGTTCTTTTAAAAAAATAG", "aa": "MDRVYNFFFNHAVVTDQVSQETTFAYNLFLKNSYCHLDKHELNLVISEMMIFLNFLYKTKKISLNYAKGFLTVLSFFAPFLAEELNEKCGLEPFVVKQAISLVDYQLFETAKTKVILSINGKFKAAKEFTKGSLEIDVLESFKQDKEINDILNQPIERVVYVQDRIINVLLKK", "aa_hexdigest": "2c9758d3cb5b5ec32c70d3d00cd4f996", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08264.19", "name": "Anticodon_1", "length": 132, "aa_cov": 0.6011560693641619, "hmm_cov": 0.9924242424242424, "evalue": 5.638440090621887e-09, "score": 31.171886444091797, "start": 29, "stop": 132 } ], "seq_stats": { "molecular_weight": 20140.220100000002, "isoelectric_point": 6.833490562438965 }, "id": "KMADJIBOEG_611", "locus": "GCA000292445_00589" }, { "type": "cds", "sequence": "contig_1", "start": 322498, "stop": 322701, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "TTGTTTGACAAGAAGCTAGTATCAACAAAAGAACCATTTCAAAAATTAATTAATCAGGGTATGGTGTTAGGTCCTGATGGTAAAAAGATGTCCAAATCCAAAGGTAATACCATTAACCCCACACCACTTGTTGATTCACATGGAGCAGATGCTTTAAGGTTGTACTTAATGTTTATGGGCCCAATTAGTGCTAGTTTAACTTGA", "aa": "MFDKKLVSTKEPFQKLINQGMVLGPDGKKMSKSKGNTINPTPLVDSHGADALRLYLMFMGPISASLT", "aa_hexdigest": "eb115c2118611b228a8c3c32605c2634", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 52, "aa_cov": 0.7761194029850746, "hmm_cov": 0.9807692307692307, "evalue": 2.0049866828743208e-17, "score": 58.140174865722656, "start": 8, "stop": 59 } ], "seq_stats": { "molecular_weight": 7295.5466000000015, "isoelectric_point": 9.698381996154787 }, "id": "KMADJIBOEG_612", "locus": "GCA000292445_00590" }, { "type": "cds", "sequence": "contig_1", "start": 322882, "stop": 323370, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28", "PFAM:PF13603.12" ], "nt": "TTGATCCCTGTCTATGTTGCTAACTATGTAATTGAAGGATATGGAACAGATGCTATTATGGGTGTTGGAGCACACAATGAAAATGATAACTTCTTCGCACGTAAACAAAAGTTGAAAATTATCAACGTCATTGATAAAAAAGAACGGCTGCAAAATTCATTTGCATATAACGGATTAACAACTAAAGAAGCACAAGTAGCTATTACTAATGAGTTAATTTCACAAAATAAAGCGAAATTAACAACTGTATATAAACTGCGTGATTGGATCTTCAGTAGACAGCGTTATTGGGGCGAACCTTTTCCAATTATTTTTGATGAAAATAACACTCCTCATTTGGTAGAACAACTCCCTGTTGAATTACCCTTACTTGAGAATTACAAACCAGATGGAAGTGGTAATTCTCCACTAATGAGAAATCAAGCTTGGGTAAACATAGTCAAAGATAACATCCATTACCAAAGGGAAACTAATACCATGCCCCAATGA", "aa": "MIPVYVANYVIEGYGTDAIMGVGAHNENDNFFARKQKLKIINVIDKKERLQNSFAYNGLTTKEAQVAITNELISQNKAKLTTVYKLRDWIFSRQRYWGEPFPIIFDENNTPHLVEQLPVELPLLENYKPDGSGNSPLMRNQAWVNIVKDNIHYQRETNTMPQ", "aa_hexdigest": "6d1f30fdb06f2ff605d0193935d50714", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 37, "aa_cov": 0.22839506172839505, "hmm_cov": 1.0, "evalue": 2.3405687729498332e-07, "score": 24.979543685913086, "start": 75, "stop": 111 }, { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 57, "aa_cov": 0.345679012345679, "hmm_cov": 0.9824561403508771, "evalue": 5.913281771552591e-18, "score": 60.13599395751953, "start": 2, "stop": 57 } ], "seq_stats": { "molecular_weight": 18758.094199999996, "isoelectric_point": 6.838776588439941 }, "id": "KMADJIBOEG_613", "locus": "GCA000292445_00591" }, { "type": "cds", "sequence": "contig_1", "start": 324028, "stop": 324213, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGTTCCCCTATCCCTCAGCAGCAGGATTACATTTAGGACATGTTAGAGCTTATACTATCACTGATGTAATAAGTAGGTATTACAAAGCTAAAGGATTTAATGTGATCCATCCGATTGGTTTTGATGCTTTTGGTTTACCTGCTGAACAGTATGCTATTAACTCTAATCAAAACCCTGGCAGTTGA", "aa": "MFPYPSAAGLHLGHVRAYTITDVISRYYKAKGFNVIHPIGFDAFGLPAEQYAINSNQNPGS", "aa_hexdigest": "92924fc03ef03d109b9b91edf6dabae5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 47, "aa_cov": 0.7540983606557377, "hmm_cov": 1.0, "evalue": 9.236943764645999e-10, "score": 32.897457122802734, "start": 5, "stop": 50 } ], "seq_stats": { "molecular_weight": 6684.463900000002, "isoelectric_point": 8.122839164733886 }, "id": "KMADJIBOEG_614", "locus": "GCA000292445_00592" }, { "type": "cds", "sequence": "contig_1", "start": 324322, "stop": 324444, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTCACTTTTTTATCCCTGTTGGCTTTGGGTTGTTATTTGGTTTGATTATTGCGATTATTGTTGACATACTCTATCGCTTAACTAAATATGCAATCAAGCGTTCTTATCAAAGCAATTAG", "aa": "MVHFFIPVGFGLLFGLIIAIIVDILYRLTKYAIKRSYQSN", "aa_hexdigest": "57dde39cf5d183e04b35dde3385cf6f5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4631.5682000000015, "isoelectric_point": 9.819775962829588 }, "id": "KMADJIBOEG_615", "locus": "GCA000292445_00593" }, { "type": "cds", "sequence": "contig_1", "start": 324715, "stop": 324915, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTAACCGCAAAGTAGAGATTGATAACTTTACTAAAAATCAACTTTACTTTCAAAATTTACACAGGGTTTACACTGGATTTATGGAAGCGGTTTGTAATGATTTTGGGATTAATTACTGTATTATAGATGCAAAACTACCAATAGTAACTATTATTAAAATGATCCTTGAAAAATTAAAGTTACAAAAGTTAGATTGA", "aa": "MRNRKVEIDNFTKNQLYFQNLHRVYTGFMEAVCNDFGINYCIIDAKLPIVTIIKMILEKLKLQKLD", "aa_hexdigest": "91b11843a60bdb49a0903a8ab9ca84aa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7866.274499999997, "isoelectric_point": 9.031456565856931 }, "id": "KMADJIBOEG_616", "locus": "GCA000292445_00594" }, { "type": "cds", "sequence": "contig_1", "start": 325012, "stop": 325389, "strand": "-", "frame": 1, "gene": "dck", "product": "putative protein MG268", "db_xrefs": [ "COG:COG1428", "COG:F", "GO:0005524", "GO:0005737", "GO:0019136", "SO:0001217", "UniRef:UniRef50_P75396", "UniRef:UniRef90_P47510" ], "nt": "ATGCAACTAAAAAAGCCCCATTTTCAACCAAATAAAATTGCTAATTGTATTGTGATCGGGGGAATGATTGCTTTAGGAAAAACCACCATTGCTAATACATTAGCTAACCACATTCAAGCTGCAAAAGTTGTTTGTGAATTGGAAACTAATGACCAGTTGGTTGAACTTTTACTAGCAAAGATGTATGAACGTAGTGATGAATTGCTCTATTCACCTTTGTTTCAGCTTTATTTTACGCTTAATCGCTTTGGTAAATACCAAACCAATTGCAACACTATCAATCCAACCATCTTTGATCGTTCTATCTTTGAAGACTGGTTGTTTGCTAAGCACAACATCATTCGTCCTGCAGTCTTTTCATACTATAACCAACTGTGA", "aa": "MQLKKPHFQPNKIANCIVIGGMIALGKTTIANTLANHIQAAKVVCELETNDQLVELLLAKMYERSDELLYSPLFQLYFTLNRFGKYQTNCNTINPTIFDRSIFEDWLFAKHNIIRPAVFSYYNQL", "aa_hexdigest": "8c876170f7e31d252912325ea40501d8", "start_type": "ATG", "rbs_motif": null, "genes": [ "dck" ], "truncated": "5-prime", "pseudogene": { "start": 324709, "stop": 325389, "observations": { "mutation": [ 324717, 324942, 325014 ], "stop": [ 324717, 324942, 325014 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47510", "query_cov": 1.0, "subject_cov": 0.5482456140350878, "identity": 0.992, "score": 256.0, "evalue": 5.99e-85, "gene_start": 1, "gene_end": 125, "reference_start": 1, "reference_end": 125, "reference_sequence": "MQLKKPHFQPNKIANCIVIGGMIALGKTTIANTLANHIQAAKVVCELETNDQLVELLLAKMYERSDELLYSPLFQLYFTLNRFGKYQNNCNTINPTIFDRSIFEDWLFAKHNIIRPAVFSYYNQLWNRLAKELVNKHGVPNLYVILDGDWKLFEKRLFMRNRKVEIDNFTKNQLYFQNLHRVYTGFMEAVCNDFGINYCIIDAKLPIVTIIKMILEKLKLQKLDWKFI" }, "paralog": false, "identity": 0.9823008849557522, "score": 447.0, "evalue": 8.79e-163, "description": "Internal stop codon at 324717, 324942, 325014. Nonsense mutation around 324717, 324942, 325014.", "psc": { "uniref90_id": "UniRef90_P47510", "gene": "Dck", "product": "Uncharacterized protein MG268", "uniref50_id": "UniRef50_P75396", "cog_id": "COG1428", "cog_category": "F", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0019136" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47510", "UniRef:UniRef50_P75396", "COG:COG1428", "COG:F", "GO:0005737", "GO:0005524", "GO:0019136" ] }, "pscc": { "uniref50_id": "UniRef50_P75396", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75396" ], "product": "Uncharacterized protein MG268 homolog" } }, "id": "KMADJIBOEG_617", "locus": "GCA000292445_00595" }, { "type": "ncRNA", "class": null, "sequence": "contig_1", "start": 325425, "stop": 325520, "strand": "-", "gene": "Bacteria_small_SRP", "product": "Bacterial small signal recognition particle RNA", "score": 61.6, "evalue": 5.5e-08, "db_xrefs": [ "RFAM:RF00169", "SO:0000655" ], "nt": "ATTTAGAACCATCACATCATTAGAGTCGAATCATGTCAGGCCAGAAATGGAGCAGCATTAAGACTTGTCAGTGAGTGTGATGGTTTTTTATTAAAA", "id": "KMADJIBOEG_42", "locus": "GCA000292445_00596" }, { "type": "cds", "sequence": "contig_1", "start": 325540, "stop": 326562, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MG269", "db_xrefs": [ "RefSeq:WP_014894012.1", "SO:0001217", "UniParc:UPI00027B3AC5", "UniRef:UniRef100_UPI00027B3AC5", "UniRef:UniRef50_Q49407", "UniRef:UniRef90_Q49407" ], "nt": "ATGAATAATTTTGATTACTATAGAGACTTTGATGACTTTCAAAGAAGAGAAACTATTAACTTCTTTTTAGCCAAGTTTCCCTTTGCTTCCAAAGCCCAGCTTGATGTTTTTTTAGAACAAGCAAAAACTGCTTATGTCAAACTACGAACAAGCAACCCAAAAAATGTTGATTGGAATCAGATGCTTGTTTTGTTATTAAAAAAGTTTGGTCCTGAAGCGAGAAGTGAAAAAGAACGTTTAAAAAGGGTCTTAAGTTTGCAAGAACAACTTAAGGTACGCTTGGAAGGAGAGATTAACCGTCAAGTTCAACAGAACAGCGAACTTTTCTCACAACTCAAACAAAGTGAAAGTGAAATCATCCAAATGCAGCAGCTGGTAGAAGCAAAAGAACACCAAATTGAAGCATTAAATAAACAGTTACATGCAATAAAAGAAGCCAATAAGAAACTAATTGAAGAACATGAAAGTATTAATGTAGAGGAATTGTTAAAGGAATATGAAGTGCAGTGTAATGAAGCAATTTATAAACGTGATCAACACATCCAAACCGTATTTGAAGACAAGTTAGCACTTAAAGATGGGGAAATTAGTGAAACCCAATCACTTTTAAAAACCGCTGAAAAAGAAAAGCAAGCATTAAAAAAAGCATATAAGTTAGTTGTAAATTCGCTTCAAAAACACCAAAAACTCACCAACGAAATAGAAATTGATTTCACCAAGCTTGATGAGATTATTGCAACCATTTTTGATGAAACTAAGAATCCAAAGACTGGCTTTACTAACTTCATTAAGCAGTTTGAAAAAACCAAAGCAAAACTAACAAAAAAGATAGCTGAAATTACTAAACTTGATCATTCAACGCCAACAAATTATCAACAAGAAACTCCAGCGTCACAACAGCAATTAGATCAAGAGAATGAACCGATCAAACCATCTAAAAAATCAAATTCAAGTTCATTGCCTAGAGGCACTACACAACCCAAATCCAATTCTATTAACAGGGTTAGTAAATTAATTGATTAA", "aa": "MNNFDYYRDFDDFQRRETINFFLAKFPFASKAQLDVFLEQAKTAYVKLRTSNPKNVDWNQMLVLLLKKFGPEARSEKERLKRVLSLQEQLKVRLEGEINRQVQQNSELFSQLKQSESEIIQMQQLVEAKEHQIEALNKQLHAIKEANKKLIEEHESINVEELLKEYEVQCNEAIYKRDQHIQTVFEDKLALKDGEISETQSLLKTAEKEKQALKKAYKLVVNSLQKHQKLTNEIEIDFTKLDEIIATIFDETKNPKTGFTNFIKQFEKTKAKLTKKIAEITKLDHSTPTNYQQETPASQQQLDQENEPIKPSKKSNSSSLPRGTTQPKSNSINRVSKLID", "aa_hexdigest": "78f48afeffa01f5df8485874462e015f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3AC5", "ncbi_nrp_id": "WP_014894012.1", "uniref100_id": "UniRef100_UPI00027B3AC5", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3AC5", "RefSeq:WP_014894012.1", "UniRef:UniRef100_UPI00027B3AC5" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3AC5", "uniref90_id": "UniRef90_Q49407" }, "psc": { "uniref90_id": "UniRef90_Q49407", "product": "Uncharacterized protein MG269", "uniref50_id": "UniRef50_Q49407" }, "pscc": { "uniref50_id": "UniRef50_Q49407", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49407" ], "product": "Uncharacterized protein MG269" }, "genes": [], "id": "KMADJIBOEG_618", "locus": "GCA000292445_00597" }, { "type": "cds", "sequence": "contig_1", "start": 326555, "stop": 326941, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MG269-1", "db_xrefs": [ "RefSeq:WP_009885901.1", "SO:0001217", "UniParc:UPI0000557E21", "UniRef:UniRef100_Q49329", "UniRef:UniRef50_Q49329", "UniRef:UniRef90_Q49329" ], "nt": "ATGGATAGTTTTGTTCAAAAATACACAAATGGCTTCAAATCAATTTTAAACAAGGTTGAAAAAACTGATTTTGCAACTATTAAAAGTGAATTTCAATATAACCAAGCTAACTTGGAATGGGTTGAAAGTAAAGTTAGTGATCTTAACAACTATTTACTAGATCCTAACCAGTTTAGTGATGTAGTTAGTTTTAAAAAAATTGCTAATGAAAAGTTAGATTTGTTTGTTAAAAACCATGGCAATAAACTCCCGTTTTTTCTCTTTACTAGTTTTGTTTTAGCAATCTTTAGTTTTGTTAGTGTTTATGTTAGACACCATTATGATTTAGACTTTAATGATCCTGATGCTATCATTAGCTTCTTTAGAGAATTAGCTTTCCATGAATAA", "aa": "MDSFVQKYTNGFKSILNKVEKTDFATIKSEFQYNQANLEWVESKVSDLNNYLLDPNQFSDVVSFKKIANEKLDLFVKNHGNKLPFFLFTSFVLAIFSFVSVYVRHHYDLDFNDPDAIISFFRELAFHE", "aa_hexdigest": "1146b1a58ef06b100147b7ea7666ba20", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000557E21", "ncbi_nrp_id": "WP_009885901.1", "uniref100_id": "UniRef100_Q49329", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000557E21", "RefSeq:WP_009885901.1", "UniRef:UniRef100_Q49329" ] }, "ips": { "uniref100_id": "UniRef100_Q49329", "uniref90_id": "UniRef90_Q49329" }, "psc": { "uniref90_id": "UniRef90_Q49329", "product": "Uncharacterized protein MG269.1", "uniref50_id": "UniRef50_Q49329" }, "pscc": { "uniref50_id": "UniRef50_Q49329", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49329" ], "product": "Uncharacterized protein MG269.1" }, "genes": [], "id": "KMADJIBOEG_619", "locus": "GCA000292445_00598" }, { "type": "cds", "sequence": "contig_1", "start": 327231, "stop": 327341, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAATTTTTTCACTGTTACTGAAAAAGCAGAAACAATTGTTTTAACTAAAGATGCACTAGCAAAGGTTGAAAAAAGAGCAAAAGAACACTTTCAATCATGGGAGTGA", "aa": "MINFFTVTEKAETIVLTKDALAKVEKRAKEHFQSWE", "aa_hexdigest": "cd5d4224ba381beb8927f39d7f8f2154", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4239.8462, "isoelectric_point": 6.556798362731934 }, "id": "KMADJIBOEG_620", "locus": "GCA000292445_00599" }, { "type": "cds", "sequence": "contig_1", "start": 327366, "stop": 327887, "strand": "-", "frame": 2, "gene": null, "product": "biotin/lipoate A/B protein ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0037DCED81" ], "nt": "TTGCTAACTGAATTTAGAAAAAATGAGTTAGTTAAGGTCATCTACTTTTGGCAGAACGCTAACACTATTGTGGTGGGAAGAAACCAAAATACTTATGCTGAGGTTAACTTAAAGGAGTTGGAAAGTGATAAGGTTAACTTGTTTAGACGTTTTTCAGGCGGGGGAGCGGTGTTTCATGACCTTGGTAACATCTGTTTTTCTATTATTTTGCCAAGAACAGGTAAAGTGATGGAAAATGCTTATGAACAAACTACAAGAAATGTGGTGAAGTTCTTAAATAGCTTAAATGTACCTGCTGTATTTCATGGTCGTAATGACCTTGAGATTGATAACAAGAAGTTTTCTGGGTTAGCTGAATATATCGCTAAAGACAGGTTATTAGTCCATGGAACATTATTGTTTGACACTGACTTTTCTAAGTTAGCAAAGTATTTAAATGTTGATAAGACCAAGATAGCAAGTAAGGGTGTTGACAGTGTTGCTAAGCGCGTTGTTAATGTAAAGGAGTATTTACCAAATTGA", "aa": "MLTEFRKNELVKVIYFWQNANTIVVGRNQNTYAEVNLKELESDKVNLFRRFSGGGAVFHDLGNICFSIILPRTGKVMENAYEQTTRNVVKFLNSLNVPAVFHGRNDLEIDNKKFSGLAEYIAKDRLLVHGTLLFDTDFSKLAKYLNVDKTKIASKGVDSVAKRVVNVKEYLPN", "aa_hexdigest": "93928871902e4190373bd754b0b21fd8", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0037DCED81", "query_cov": 0.8901734104046243, "subject_cov": 0.8461538461538461, "identity": 0.526, "score": 164.0, "evalue": 9.35e-49, "valid": false, "product": "biotin/lipoate A/B protein ligase family protein", "uniref50_id": "UniRef50_UPI0037DCED81" }, "pscc": { "uniref50_id": "UniRef50_UPI0037DCED81", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0037DCED81" ], "product": "biotin/lipoate A/B protein ligase family protein" }, "genes": [], "id": "KMADJIBOEG_621", "locus": "GCA000292445_00600" }, { "type": "cds", "sequence": "contig_1", "start": 327964, "stop": 328416, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02852.28" ], "nt": "ATGATGTTGGCACACTACGCTTACCAACAGGGCAGATATGCTGTTGATCAAATTTTGAACCAAAACCAGGTAAAGCCTGCTGAAAAAAACAAGTGTCCTGCTTGTATTTACACAAATCCTGAAGTTGCTTTTGTAGGTTATAGTGAGATGGAATTGCAAAAAGAAAAGATTGATTATGTCAAATCTTCTTTGCCATTTATTTATAGTGGTAAAGCAATTGCAGATCATGAAACCAATGGCTTTGTCAAGATGATGTTTAATCCTAAAACTGGTGCTATCTTAGGTGGATGTATTATTGCTAGCACTGCTAGTGATATTATCGCTGAGCTTGCTTTGGTGATGGAAAACAACCTCACTGTGTTTGATATTGCCAATTCTATCTCACCCCATCCTACCATGAATGAAATGGTAACTGATGTTTGTAAAAAAGCGATCTTTGATTACTTTAGTTAA", "aa": "MMLAHYAYQQGRYAVDQILNQNQVKPAEKNKCPACIYTNPEVAFVGYSEMELQKEKIDYVKSSLPFIYSGKAIADHETNGFVKMMFNPKTGAILGGCIIASTASDIIAELALVMENNLTVFDIANSISPHPTMNEMVTDVCKKAIFDYFS", "aa_hexdigest": "c62ea9a8e936ade52f2dd2661c253f51", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02852.28", "name": "Pyr_redox_dim", "length": 107, "aa_cov": 0.7133333333333334, "hmm_cov": 1.0, "evalue": 2.070889185521157e-33, "score": 110.02239227294922, "start": 33, "stop": 139 } ], "seq_stats": { "molecular_weight": 16675.07159999999, "isoelectric_point": 5.291562461853027 }, "id": "KMADJIBOEG_622", "locus": "GCA000292445_00601" }, { "type": "cds", "sequence": "contig_1", "start": 328575, "stop": 328976, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00070.33", "PFAM:PF07992.20" ], "nt": "GTGCAAGTAAACAACACAACTTACACCACTAACAACATTATTGTTGCAACCGGATCAAGACCAAGATACTTAACTTTACCAGGGTTTGAAAAAGCACAACAAGCTGGGTTTATCATTGACTCAACCCAAGCTTTGGCTTTAGAGGGAGTACCTAAGAAGTTTGTTGTAGTTGGGGGAGGTGTGATTGGGGTTGAGTTTGCTTTTTTATTTGCTTCATTAGGGAGTGAAGTGACCATTATCCAAGGTGTTGATAGGATTTTGGAGGTTTGTGATAGTGATGTTTCTGAACTGATAAGTAAAACCTTAAAAACAAAGGAGTTCAGATTATTACCAATGCTCATGTTGTTAGAGCTGAAAACAACCAACTGTTTTACACAGTTAATGGAGTTGAACAGTCTGTAA", "aa": "MQVNNTTYTTNNIIVATGSRPRYLTLPGFEKAQQAGFIIDSTQALALEGVPKKFVVVGGGVIGVEFAFLFASLGSEVTIIQGVDRILEVCDSDVSELISKTLKTKEFRLLPMLMLLELKTTNCFTQLMELNSL", "aa_hexdigest": "b23a30dc729c5ac400fd929fd55ba046", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00070.33", "name": "Pyr_redox", "length": 58, "aa_cov": 0.43609022556390975, "hmm_cov": 1.0, "evalue": 1.6093587739926767e-15, "score": 52.57574462890625, "start": 53, "stop": 110 }, { "id": "PF07992.20", "name": "Pyr_redox_2", "length": 106, "aa_cov": 0.7894736842105263, "hmm_cov": 1.0, "evalue": 1.4352621317574445e-31, "score": 105.07221984863281, "start": 4, "stop": 108 } ], "seq_stats": { "molecular_weight": 14562.852599999991, "isoelectric_point": 5.0969457626342765 }, "id": "KMADJIBOEG_623", "locus": "GCA000292445_00602" }, { "type": "cds", "sequence": "contig_1", "start": 329100, "stop": 329336, "strand": "-", "frame": 2, "gene": null, "product": "FAD-dependent oxidoreductase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X1ZRV2" ], "nt": "ATGGATTATGATCTAATTATTTTGGGTGCTGGCCCTGCTGGTTATATTGCTGCGGAGTATGCTGGCAAACATAAACTTAAAACCCTAGTGATTGAAAAGCAATACTTTGGTGGGGTGTGTTTAAATGTTGGGTGTATCCCAACTAAAACGTTGTTAAAAAGAGCAAAGATTATTGATTATTTAGTTCATGCCAAAGATTATGGTATCACTATTAATGGTCAAGCTAAACTTGATTGA", "aa": "MDYDLIILGAGPAGYIAAEYAGKHKLKTLVIEKQYFGGVCLNVGCIPTKTLLKRAKIIDYLVHAKDYGITINGQAKLD", "aa_hexdigest": "8b23540e0848f0b7478a32e15e6e2edb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0010C3BE0E", "query_cov": 0.9358974358974359, "subject_cov": 0.9358974358974359, "identity": 0.562, "score": 96.3, "evalue": 1.25e-24, "valid": false, "product": "FAD-dependent oxidoreductase", "uniref50_id": "UniRef50_A0A7X1ZRV2" }, "pscc": { "uniref50_id": "UniRef50_A0A7X1ZRV2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X1ZRV2" ], "product": "FAD-dependent oxidoreductase" }, "genes": [], "id": "KMADJIBOEG_624", "locus": "GCA000292445_00603" }, { "type": "cds", "sequence": "contig_1", "start": 329340, "stop": 330494, "strand": "-", "frame": 2, "gene": "pdhC", "product": "Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex", "db_xrefs": [ "COG:C", "COG:COG0508", "EC:2.3.1.12", "GO:0004742", "GO:0005737", "GO:0016407", "GO:0031405", "RefSeq:WP_014894247.1", "SO:0001217", "UniParc:UPI00027B3E2D", "UniRef:UniRef100_UPI00027B3E2D", "UniRef:UniRef50_P47514", "UniRef:UniRef90_P47514" ], "nt": "ATGGCAAATGAGTTTAAATTCACTGATGTTGGTGAGGGTTTACATGAAGGAAAAGTAACTGAAATCTTAAAACAAGTTGGTGATCAGATCAAGATAGATGAAGCTTTATTTGTTGTTGAAACTGATAAAGTTACAACCGAATTACCTTCTCCTTTTGCAGGTACAATTAGCGCTATTAATGTTAAAGTTGGTGATGTTGTTAGCATTGGTCAGGTGATGGCAGTTATTGGTGAAAAGACTAGTACACCAATTGTTGAACCAAAACCTCAACCAACTGAAGAAGTAACTAAGGTAAAAGAAGCGGGGGCTTCAGTAGTAGGGGAAATTAAGGTTTCTGATAACCTCTTTCCTATCTTTGGAGTAAAACCTCATGCAACTCCAGCTGTTAAAGACACTAAAGTTGCAAGTAGTACTAACATTACTGTAGAAACAACCCAAAAACCAGAAAGTAAAACTGAACAGAAAACCATTGCTATCTCAACAATGCGCAAAGCGATTGCAGAAGCAATGACAAAGTCGCACGCAATTATCCCAACCACTGTATTAACTTTTTATGTTAATGCAACCAAGTTAAAACAATATCGTGAAAGTGTTAATGGTTATGCTTTAAGTAAGTATTCCATGAAAATTTCTTACTTTGCTTTCTTTGTTAAAGCAATTGTTAATGCGCTTAAGAAGTTCCCTGTTTTTAACGCTAGTTATGATCCTGATCAAAACGAAATTGTTTTAAATGATGACATTAATGTAGGAATTGCTGTTGATACTGAAGAAGGTTTAATTGTCCCTAACATTAAGCAAGCCCAAACCAAATCTGTGGTTGAAATTGCCCAAGCAATTGTTGATTTAGCTAACAAAGCTAGAACAAAAAAGATTAAGTTGACTGATTTGAATAAAGGTACTATTTCAGTTACTAACTTCGGTTCATTAGGAGCAGCTGTAGGTACACCTATTATTAAGTACCCTGAGATGTGTATTGTTGCTACTGGTAATTTAGAAGAACGCATTGTTAAAGTGAAAAATGGAATTGCAGTTCATACCATCTTACCTTTAACAATAGCTGCAGACCACCGCTGGGTTGATGGGGCGGATGTTGGTAGGTTTGGTAAGGAGATTGCAAAACAAATTGAGGAATTAATTGATCTTACAGTAGCTTAA", "aa": "MANEFKFTDVGEGLHEGKVTEILKQVGDQIKIDEALFVVETDKVTTELPSPFAGTISAINVKVGDVVSIGQVMAVIGEKTSTPIVEPKPQPTEEVTKVKEAGASVVGEIKVSDNLFPIFGVKPHATPAVKDTKVASSTNITVETTQKPESKTEQKTIAISTMRKAIAEAMTKSHAIIPTTVLTFYVNATKLKQYRESVNGYALSKYSMKISYFAFFVKAIVNALKKFPVFNASYDPDQNEIVLNDDINVGIAVDTEEGLIVPNIKQAQTKSVVEIAQAIVDLANKARTKKIKLTDLNKGTISVTNFGSLGAAVGTPIIKYPEMCIVATGNLEERIVKVKNGIAVHTILPLTIAADHRWVDGADVGRFGKEIAKQIEELIDLTVA", "aa_hexdigest": "ee442c4812fd51e668c2145922d4f956", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3E2D", "ncbi_nrp_id": "WP_014894247.1", "uniref100_id": "UniRef100_UPI00027B3E2D", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3E2D", "RefSeq:WP_014894247.1", "UniRef:UniRef100_UPI00027B3E2D" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3E2D", "uniref90_id": "UniRef90_P47514" }, "psc": { "uniref90_id": "UniRef90_P47514", "gene": "pdhC", "product": "Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex", "ec_ids": [ "2.3.1.12" ], "uniref50_id": "UniRef50_P47514", "cog_id": "COG0508", "cog_category": "C", "go_ids": [ "GO:0005737", "GO:0016407", "GO:0004742", "GO:0031405" ] }, "pscc": { "uniref50_id": "UniRef50_P47514", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47514" ], "product": "Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex" }, "genes": [ "pdhC" ], "id": "KMADJIBOEG_625", "locus": "GCA000292445_00604" }, { "type": "cds", "sequence": "contig_1", "start": 330745, "stop": 330969, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02780.25" ], "nt": "GTGAAGAAAACAGGAAGACTACTTGTAGTGACTGAAGCGGTGAAAAGTTTCACTACAAGTGCAGAGATTATCACTTCAGTAACTGAAGAACTATTCACTTATCTCAAAAAAGCCCCACAACGGGTAACTGGGTTTGATATTGTTGTGCCTTTAGCTAGAGGTGAAAAATACCAGTTTGAAATTAATGCACGGGTTATTGATGCAGTTAATCAACTTTTAAAATAA", "aa": "MKKTGRLLVVTEAVKSFTTSAEIITSVTEELFTYLKKAPQRVTGFDIVVPLARGEKYQFEINARVIDAVNQLLK", "aa_hexdigest": "f5a9a38b26064ae10e52b945f1686ad8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02780.25", "name": "Transketolase_C", "length": 57, "aa_cov": 0.7702702702702703, "hmm_cov": 1.0, "evalue": 5.586313050729372e-13, "score": 44.06863021850586, "start": 1, "stop": 57 } ], "seq_stats": { "molecular_weight": 8315.6384, "isoelectric_point": 9.453788948059085 }, "id": "KMADJIBOEG_626", "locus": "GCA000292445_00605" }, { "type": "cds", "sequence": "contig_1", "start": 330994, "stop": 331725, "strand": "-", "frame": 1, "gene": "pdhB", "product": "Pyruvate dehydrogenase E1 component subunit beta", "db_xrefs": [ "COG:C", "COG:COG0022", "EC:1.2.4.1", "GO:0004739", "GO:0007584", "GO:0009083", "GO:0140032", "SO:0001217", "UniRef:UniRef50_P75391", "UniRef:UniRef90_P47515" ], "nt": "ATGTCAAAAATCCAAGTAAATAACATTGAAGCGTTAAACAACGCAATGGATCTTGCACTGGAAAGAGATCAAAACGTTGTACTCTATGGCCAGGACGCTGGTTTTGAAGGGGGTGTATTCCGTGCAACTAAAGGTTTACAACAAAAGTATGGGAGTGAAAGGGTATGGGATTGTCCTATAGCAGAAAACTCTATGGCCGGTATTGGGGTTGGGGCTGCTATAGGTGGTCTTAAACCTATTGTAGAGATCCAGTTTTCAGGCTTTTCATTCCCAGCTATGTTTCAAATCTTTGTCCATGCTGCTAGGATTAGAAACCGTTCTCGTGGTGTATATACCGCTCCACTAGTAGTGAGGATGCCAATGGGTGGGGGGATTAAAGCATTGGAACACCACAGTGAAACATTGGAAGCAATTTATGCACAGATTGCTGGGCTTAAAACAGTGATGCCATCAAATCCTTATGATACCAAAGGACTTTTTCTAGCTGCTATTGAATCACCTGATCCTGTTATCTTTTTTGAACCAAAGAAGCTTTATCGTGCTTTTCGTCAGGAGATTCCTAGTGATTATTACACTGTCCCTATTGGTGAAGCCAACTTGATTAGTGAAGGTAGTGAACTTACAATAGTTAGCTATGGTCCTACAATGTTTGATTTAATTAACTTAGTTTACAGCGGGGAATTGAAAGATAAGGGAATTGAGTTAATTGACTTGCGTACTATCTCCCCTTGA", "aa": "MSKIQVNNIEALNNAMDLALERDQNVVLYGQDAGFEGGVFRATKGLQQKYGSERVWDCPIAENSMAGIGVGAAIGGLKPIVEIQFSGFSFPAMFQIFVHAARIRNRSRGVYTAPLVVRMPMGGGIKALEHHSETLEAIYAQIAGLKTVMPSNPYDTKGLFLAAIESPDPVIFFEPKKLYRAFRQEIPSDYYTVPIGEANLISEGSELTIVSYGPTMFDLINLVYSGELKDKGIELIDLRTISP", "aa_hexdigest": "7647151f52bb756121fcb1af4f6e51f9", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FDA3C09", "query_cov": 0.934156378600823, "subject_cov": 0.9700854700854701, "identity": 0.568, "score": 270.0, "evalue": 2.56e-88, "valid": false, "product": "transketolase C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A537VMS1" }, "genes": [ "pdhB" ], "truncated": "5-prime", "pseudogene": { "start": 330745, "stop": 331725, "observations": { "mutation": [ 330996 ], "stop": [ 330996 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47515", "query_cov": 1.0, "subject_cov": 0.745398773006135, "identity": 1.0, "score": 480.0, "evalue": 5.35e-170, "gene_start": 1, "gene_end": 243, "reference_start": 1, "reference_end": 243, "reference_sequence": "MSKIQVNNIEALNNAMDLALERDQNVVLYGQDAGFEGGVFRATKGLQQKYGSERVWDCPIAENSMAGIGVGAAIGGLKPIVEIQFSGFSFPAMFQIFVHAARIRNRSRGVYTAPLVVRMPMGGGIKALEHHSETLEAIYAQIAGLKTVMPSNPYDTKGLFLAAIESPDPVIFFEPKKLYRAFRQEIPSDYYTVPIGEANLISEGSELTIVSYGPTMFDLINLVYSGELKDKGIELIDLRTISPWDKQTVFNSVKKTGRLLVVTEAVKSFTTSAEIITSVTEELFTYLKKAPQRVTGFDIVVPLARGEKYQFEINARVIDAVNQLLK" }, "paralog": false, "identity": 0.9969325153374233, "score": 635.0, "evalue": 1.07e-233, "description": "Internal stop codon at 330996. Nonsense mutation around 330996.", "psc": { "uniref90_id": "UniRef90_P47515", "gene": "pdhB", "product": "Pyruvate dehydrogenase E1 component subunit beta", "ec_ids": [ "1.2.4.1" ], "uniref50_id": "UniRef50_P75391", "cog_id": "COG0022", "cog_category": "C", "go_ids": [ "GO:0140032", "GO:0004739", "GO:0009083", "GO:0007584" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47515", "EC:1.2.4.1", "UniRef:UniRef50_P75391", "COG:COG0022", "COG:C", "GO:0140032", "GO:0004739", "GO:0009083", "GO:0007584" ] }, "pscc": { "uniref50_id": "UniRef50_P75391", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75391" ], "product": "Pyruvate dehydrogenase E1 component subunit beta" } }, "id": "KMADJIBOEG_627", "locus": "GCA000292445_00606" }, { "type": "cds", "sequence": "contig_1", "start": 331743, "stop": 332114, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00676.26" ], "nt": "ATGCAAGATACTGCTAATTACGCTAGAGGTGGTAATGGACCAGTCCTAATTGAGTTCTTCAGCTACCGGCAAGGTCCTCACACCACTTCAGATGATCCTTCTATCTACAGAACCAAACAAGAAGAGGAGGAGGGAATGAAGAGTGATCCAGTGAAGCGATTGCGAAACTTCTTGTTTGATAGATCAATTCTTAACCAAGCTCAAGAAGAAGAGATGTTCAGCAAAATTGAACAGGAAATCCAAGCTGCTTATGAAAAGATGGTACTAGATACCCCTGTATCAGTAGATGAGGTGTTTGATTACAACTACCAAGAATTAACCCCTGAACTAGTTGAACAGAAACAGATTGCAAAAAAATACTTTAAAGACTAA", "aa": "MQDTANYARGGNGPVLIEFFSYRQGPHTTSDDPSIYRTKQEEEEGMKSDPVKRLRNFLFDRSILNQAQEEEMFSKIEQEIQAAYEKMVLDTPVSVDEVFDYNYQELTPELVEQKQIAKKYFKD", "aa_hexdigest": "fab84f61476ded95ef8246d2cf0b72f1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00676.26", "name": "E1_dh", "length": 83, "aa_cov": 0.6666666666666666, "hmm_cov": 1.0, "evalue": 2.5512193620007763e-23, "score": 77.55926513671875, "start": 5, "stop": 86 } ], "seq_stats": { "molecular_weight": 14442.879499999994, "isoelectric_point": 4.650248146057129 }, "id": "KMADJIBOEG_628", "locus": "GCA000292445_00607" }, { "type": "cds", "sequence": "contig_1", "start": 332268, "stop": 332378, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00676.26" ], "nt": "ATGTTGCACTATAAAAAGCAACCTAATGTTGCTGTTACTATGATTGGTGATGGAGGTACAGCTGAAGGGGAATTTTATGAAGCGATGAACATTGCAAGCATCCACAAGTGA", "aa": "MLHYKKQPNVAVTMIGDGGTAEGEFYEAMNIASIHK", "aa_hexdigest": "210f36023b8fa0de2f1e35de77ddb3a1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00676.26", "name": "E1_dh", "length": 35, "aa_cov": 0.9722222222222222, "hmm_cov": 1.0, "evalue": 2.537189573122033e-10, "score": 34.88288497924805, "start": 2, "stop": 36 } ], "seq_stats": { "molecular_weight": 3952.4923, "isoelectric_point": 5.9887495040893555 }, "id": "KMADJIBOEG_629", "locus": "GCA000292445_00608" }, { "type": "cds", "sequence": "contig_1", "start": 332481, "stop": 332633, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00676.26" ], "nt": "ATGTTAAACTTCGCTCCTAATTTGGGAGAGGAAGCTTTACAGGTTGGAATGGGATTAGGTTTAAATGAAAATGATTGGGTTTGTCCTACGTTTCGTAGTGGGGCTTTAATGTTGTATCGTGGGGTAAAACCAGAACAACTTTTACTCTACTGA", "aa": "MLNFAPNLGEEALQVGMGLGLNENDWVCPTFRSGALMLYRGVKPEQLLLY", "aa_hexdigest": "ffc2ecc1afcb252a67b29e8a824f6f8d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00676.26", "name": "E1_dh", "length": 45, "aa_cov": 0.9, "hmm_cov": 1.0, "evalue": 1.3350488756235713e-08, "score": 29.231674194335938, "start": 5, "stop": 49 } ], "seq_stats": { "molecular_weight": 5570.4433, "isoelectric_point": 4.584314918518066 }, "id": "KMADJIBOEG_630", "locus": "GCA000292445_00609" }, { "type": "cds", "sequence": "contig_1", "start": 332649, "stop": 332819, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAATCTTGATTAAAAATAAAGTTCCAACTACCCTTTATCAGGTTTATGATAATGAGGGTAAATTAATTGATCCTAACCACAAAATTACCCTAACTGATGAACAGTTAAAACACGCTTATTACTTAATGAACTTGAGTAGAATGATGGACAAAAAGATGTTAGTTTGA", "aa": "MAILIKNKVPTTLYQVYDNEGKLIDPNHKITLTDEQLKHAYYLMNLSRMMDKKMLV", "aa_hexdigest": "397073f2a778dd24fee48785947303db", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6625.819500000001, "isoelectric_point": 9.00508899688721 }, "id": "KMADJIBOEG_631", "locus": "GCA000292445_00610" }, { "type": "cds", "sequence": "contig_1", "start": 332847, "stop": 333929, "strand": "-", "frame": 2, "gene": "nox", "product": "NADH oxidase", "db_xrefs": [ "COG:COG0446", "COG:I", "EC:1.6.3.4", "GO:0008137", "SO:0001217", "UniRef:UniRef50_Q49408", "UniRef:UniRef90_Q49408" ], "nt": "ATGAATGTTGAAAACAAGGTGACACACAAGCCTTTGGAGTTTAACTACACTGACAAATATTGTGGTAATGTTAAGAACTTAATTAGCTGTAAGTTATACCAACATGCACTTACCTTAATCGATAGTTTTCGTAAAGATAAAACCATTAAATCAGTTGCTATTGTTGGTTCTGGTTACATTGGCTTGGAACTTGCTGAAGCAGCTTGGTTATGCAAAAAGCAAGTAACAGTAATTGACTTACTTGATAAGCCTGCTGGTAATAACTTTGATCATGAGTTTACTGATGAACTTAAAAAAGTGATGCAAAAAGATGGGTTAAAACTAATGATGGGTTGCAGTGTTAAGGGCTTTGTTGTTGATAGTACAAACAACGTTGTCAAAGGAGTTGAAACTGATAAGGGAATAGTAAATGCAGACCTTGTGATCCAATCAATTGGGTTTAGACCTAGCACTAAGTTTGTTCCTAAAGATCAAAACTTTGAGTTTATTCACAACGGTTCAATTAAAGTTAATGAATTTCTCCAAGCACTAAATCATAAGGATGTTTATGTCATTGGGGGTTGTGCTGCTATTTACAATGCTGCTAGTGAACAGTATGAAAACATCGATCTTGCTACCAATGCAGTAAAGAGTGGATTAGTAGCTGCGATGCATATCATTGGTAGTAACCAAGTTAAACTCCAATCTATCGTTGGCACCAATGCACTCCATATCTTTGGTTTAAATTTAGCAGCATGTGGATTAACTGAACAGCGTGCTAAGAAGTTAGGTTTTGATGTTGGCATATCAGTTGTTGATGACAATGATCGTCCTGAGTTTATGGGCAGTTATGACAAGGTGCGTTTTAAACTTGTATATGACAAGAAAACCCTAAGAATTTTAGGAGCACAACTCCTTTCTTGGAATACCAATCACAGTGAGATTATTTTCTATATTGCACTTGCAATCCAAAAGCAGATGTTACTAACTGAACTGGGTTTAGTGGATGTTTATTTTCTTCCACATTACAACAAACCGTTTAACTTTGTGTTAGCAACTGTTTTACAAGCACTTGGTTTTAGCTATTACATTCCTAAAAAATAG", "aa": "MNVENKVTHKPLEFNYTDKYCGNVKNLISCKLYQHALTLIDSFRKDKTIKSVAIVGSGYIGLELAEAAWLCKKQVTVIDLLDKPAGNNFDHEFTDELKKVMQKDGLKLMMGCSVKGFVVDSTNNVVKGVETDKGIVNADLVIQSIGFRPSTKFVPKDQNFEFIHNGSIKVNEFLQALNHKDVYVIGGCAAIYNAASEQYENIDLATNAVKSGLVAAMHIIGSNQVKLQSIVGTNALHIFGLNLAACGLTEQRAKKLGFDVGISVVDDNDRPEFMGSYDKVRFKLVYDKKTLRILGAQLLSWNTNHSEIIFYIALAIQKQMLLTELGLVDVYFLPHYNKPFNFVLATVLQALGFSYYIPKK", "aa_hexdigest": "e74610d8cebdc3040e2c5c5724d63628", "start_type": "ATG", "rbs_motif": null, "genes": [ "nox" ], "truncated": "3-prime", "pseudogene": { "start": 332847, "stop": 334229, "observations": { "mutation": [ 333941 ], "stop": [ 333941 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49408", "query_cov": 1.0, "subject_cov": 0.7531380753138075, "identity": 0.997, "score": 714.0, "evalue": 3.88e-258, "gene_start": 1, "gene_end": 360, "reference_start": 119, "reference_end": 478, "reference_sequence": "MKKVIVIGINHAGTSFIRTLLSKSKDFKVNAYDRNTNISFLGCGIALAVSGVVKNTDDLFYSNPEELKQMGANIFMSHDVTNIDLIKKQVTVRDLTSNKEFTDQFDQLVIASGAWPICMNVENKVTHKPLEFNYTDKYCGNVKNLISCKLYQHALTLIDSFRKDKTIKSVAIVGSGYIGLELAEAAWLCKKQVTVIDLLDKPAGNNFDHEFTDELEKVMQKDGLKLMMGCSVKGFVVDSTNNVVKGVETDKGIVNADLVIQSIGFRPSTKFVPKDQNFEFIHNGSIKVNEFLQALNHKDVYVIGGCAAIYNAASEQYENIDLATNAVKSGLVAAMHIIGSNQVKLQSIVGTNALHIFGLNLAACGLTEQRAKKLGFDVGISVVDDNDRPEFMGSYDKVRFKLVYDKKTLRILGAQLLSWNTNHSEIIFYIALAIQKQMLLTELGLVDVYFLPHYNKPFNFVLATVLQALGFSYYIPKK" }, "paralog": false, "identity": 0.9891304347826086, "score": 905.0, "evalue": 0.0, "description": "Internal stop codon at 333941. Nonsense mutation around 333941.", "psc": { "uniref90_id": "UniRef90_Q49408", "gene": "nox", "product": "NADH oxidase", "ec_ids": [ "1.6.3.4" ], "uniref50_id": "UniRef50_Q49408", "cog_id": "COG0446", "cog_category": "I", "go_ids": [ "GO:0008137" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49408", "EC:1.6.3.4", "UniRef:UniRef50_Q49408", "COG:COG0446", "COG:I", "GO:0008137" ] }, "pscc": { "uniref50_id": "UniRef50_Q49408", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49408" ], "product": "NADH oxidase" } }, "id": "KMADJIBOEG_632", "locus": "GCA000292445_00611" }, { "type": "cds", "sequence": "contig_1", "start": 333939, "stop": 334283, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07992.20" ], "nt": "ATGAAAAAAGTGATTGTGATTGGAATAAATCACGCTGGTACTAGTTTTATTAGAACTTTACTTTCAAAAAGTAAGGACTTTAAGGTTAACGCTTATGATAGAAACACAAACATCTCGTTTCTGGGGTGTGGAATTGCACTTGCTGTTAGTGGTGTTGTTAAAAACACTGATGATCTTTTCTATTCCAACCCTAAGGAGTTGAAACAGATGGGCGCTAACATCTTTATGAGTCATGATGTTATTAACATTGATCTAATCAAAAAACAGGTAAAAGTTAGAGATTTAACATCAAATAAAGAGTTCACTGATCAGTTTGATCAACTAGTAATCGCTTCAGGAGCATGA", "aa": "MKKVIVIGINHAGTSFIRTLLSKSKDFKVNAYDRNTNISFLGCGIALAVSGVVKNTDDLFYSNPKELKQMGANIFMSHDVINIDLIKKQVKVRDLTSNKEFTDQFDQLVIASGA", "aa_hexdigest": "30c66f382ae7ff2b122c3df6512f52d1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07992.20", "name": "Pyr_redox_2", "length": 115, "aa_cov": 0.9912280701754386, "hmm_cov": 0.9478260869565217, "evalue": 1.2991258000768169e-17, "score": 59.26300811767578, "start": 2, "stop": 114 } ], "seq_stats": { "molecular_weight": 12624.46799999999, "isoelectric_point": 9.30106334686279 }, "id": "KMADJIBOEG_633", "locus": "GCA000292445_00612" }, { "type": "cds", "sequence": "contig_1", "start": 334366, "stop": 334944, "strand": "-", "frame": 1, "gene": "apt", "product": "adenine phosphoribosyltransferase", "db_xrefs": [ "COG:COG0503", "COG:F", "EC:2.4.2.7", "GO:0002055", "GO:0003999", "GO:0005737", "GO:0006166", "GO:0006168", "GO:0016208", "GO:0044209", "KEGG:K00759", "SO:0001217", "UniRef:UniRef50_P75388", "UniRef:UniRef90_P47518" ], "nt": "TTGCAGGAATTAACAGTTTTTAAAAAAAAGATATTTATGGATCAAAACTTTAAGTTGCTTGATCAAGCCATCAAGCGCTTTGAAAATTTTCCCAACCAAGGTACATCGTTTTATGACATTACCCCAGTATTTTCCAATCCCCAACTATTTAATTTTGTGCTAACCCAAATGGCACAGTTTATTAAAGCTATTAATGCAGAAGCGATAGTATGTCCTGAAGCGAGGGGTTTTATCTTTGGGGGAGCATTAGCTTCTAAAACCCAACTCCCGTTAGTATTGGTTAGAAAAGCCAATAAACTCCCAGGGCAATTAATTAGTGCTAGCTATGATTTGGAGTACAGAAAACATGCTGTATTGGAGATGTCAACCACTTCATTAATCCAAGCTAATAATGCTAAAAGGTGTGTTATTGTTGATGATGTACTTGCCACTGCTGGAACAGTTGCTGCTATTGACCAATTACTTAAACAGTTAAATGGTGAAACTGTGGGATATTGTTTTTTAATTGAGCTGAAAAAACTCAATGGTAAAGCTAAGTTACAACCAAATGTGGTTAGCAAGATTTTATTACATTACTAG", "aa": "MQELTVFKKKIFMDQNFKLLDQAIKRFENFPNQGTSFYDITPVFSNPQLFNFVLTQMAQFIKAINAEAIVCPEARGFIFGGALASKTQLPLVLVRKANKLPGQLISASYDLEYRKHAVLEMSTTSLIQANNAKRCVIVDDVLATAGTVAAIDQLLKQLNGETVGYCFLIELKKLNGKAKLQPNVVSKILLHY", "aa_hexdigest": "7bfc30c69ea0dac34a5b6e50ee0f00a1", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47518", "query_cov": 0.9375, "subject_cov": 1.0, "identity": 0.9940000000000001, "score": 350.0, "evalue": 5.83e-122, "valid": true, "gene": "apt", "product": "adenine phosphoribosyltransferase", "ec_ids": [ "2.4.2.7" ], "uniref50_id": "UniRef50_P75388", "cog_id": "COG0503", "cog_category": "F", "kegg_orthology_id": "K00759", "go_ids": [ "GO:0002055", "GO:0003999", "GO:0005737", "GO:0006166", "GO:0006168", "GO:0016208", "GO:0044209" ] }, "pscc": { "uniref50_id": "UniRef50_P75388", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75388" ], "product": "Adenine phosphoribosyltransferase" }, "genes": [ "apt" ], "id": "KMADJIBOEG_634", "locus": "GCA000292445_00613" }, { "type": "cds", "sequence": "contig_1", "start": 335036, "stop": 335722, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACACAGACTTTTTTATAACCAAAAACCAACATGATTTCTTTTTGAAAAACAACTGGAAGTTTTTGTTTGTATGGCTAATCTTGTTAGCTATAGGAGTTGTAATGCTAGTTTTATATCTTGTTCAACCTAACCTGTTAGGAGAATTTTTAGCAGCAGATGTTGAATCAAGTAACGGGATTATTGCTGGGATAGGAATAGTTAGTTTACTGTACTTAGCTTATTGTTTGATTAGATATGGTGTTGCTTATTGTTTAAGTTATTTGGTTAGCTTTATCTTGTTATGTAGTGGTTTGTTTGCAGTGATGTATCTAACTAACTATCTGTTTAGTATAGACCAATCAACAATCCAGTTAATTACCTTTGTTTATCTGTTTTGGCTCTTTTTTGCAGCTAAGGTATCACAAACAACAACTTGGACTTACTTTTACTGGTTTAAAAGGAGCTTAAAGGACAAGGTATTTATCAAAAACTTATTTAACGATAACTTCAACTCGCAGTGGAAGATAGATCTGATTGAAAGTTCTAGCTTAGTTTTCATTTTTATTATCTACAGTGGTTTTAACTTTGGTGGGATTGATGGCAACTTTAACCTTGTGATCTTTTATCTAATTGCTATTGTTGGTTTATTTGATGTTGCAACTGCTTTTTTACCAATGTTTTGCTTTGGTTTAATCAATGGTTGA", "aa": "MNTDFFITKNQHDFFLKNNWKFLFVWLILLAIGVVMLVLYLVQPNLLGEFLAADVESSNGIIAGIGIVSLLYLAYCLIRYGVAYCLSYLVSFILLCSGLFAVMYLTNYLFSIDQSTIQLITFVYLFWLFFAAKVSQTTTWTYFYWFKRSLKDKVFIKNLFNDNFNSQWKIDLIESSSLVFIFIIYSGFNFGGIDGNFNLVIFYLIAIVGLFDVATAFLPMFCFGLING", "aa_hexdigest": "9a4891a76217b07c8c1a4a4160d0d815", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26266.718400000016, "isoelectric_point": 5.678522300720215 }, "id": "KMADJIBOEG_635", "locus": "GCA000292445_00614" }, { "type": "cds", "sequence": "contig_1", "start": 336017, "stop": 336451, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTAGTGATGGTACATACACCCCACCAAACTTTGTAACAGCAGCTACTGTTAAAGAGCTTTTAACAAATCCATTTCCCACAGCAGCTACCATTGCAACTGCAAAAACAAGTCTGGTTAACGCTCCACTTGCAAATACCATCACAGATGTTTCTAATTTTGCTTCTAGTTTTATTGCGTTTGGTATCATCGTTCTTATTGCTGCTGTTCTATTAACACTACGATACAAGCTCTTAGGGTTATACAAAGCATTAGCTTTAGGGTTAAGTGTGGTGTCATCACTGGTTATCTTTAGTGCAGTTGGTGGAGTTGTTGATGTCTTTTCTTTTGTGGGCATCTTCTTTGTTATTGCCATCAACCTAATTAATCTCTTGAGTTTAGGAGAGTTATTCTTAAGAAATATTAGAAATAATGCTTCTATTATCGAATCGTGA", "aa": "MSSDGTYTPPNFVTAATVKELLTNPFPTAATIATAKTSLVNAPLANTITDVSNFASSFIAFGIIVLIAAVLLTLRYKLLGLYKALALGLSVVSSLVIFSAVGGVVDVFSFVGIFFVIAINLINLLSLGELFLRNIRNNASIIES", "aa_hexdigest": "7a361ed3cafedc7a842d3582fdc6ca41", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15142.639899999991, "isoelectric_point": 8.06391506195068 }, "id": "KMADJIBOEG_636", "locus": "GCA000292445_00615" }, { "type": "cds", "sequence": "contig_1", "start": 336995, "stop": 337450, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTAATGGGGGATTACCTTCTGTTACTCTCACCTTTGAACAAAACCGTGAGCAAGCCAAAACCCGCCAAAGAAAAAGACAAGTTGATGCTCAGGTTAGAAGAAATGCAATTAGTTCAGTTGAACACAACTACCAACTTTCACTTGAAACAACTGATGGAGTGGTTTTATTTGATTCATTGGATAATAATTTTATTAATGCTTCCATTAGAGCAGTTGTTCCCCAAAACACTTCTGTAAACAGTGCTTTAACCTTTGAATACAAGTTAAATAAAAATGTAGTTACCAAAGAAAGTTTACACACCGATTTTCTTGATTTTATCAACTCTAAAAACTTATCAAGTTCTGATTATAATACTGGTAATGGGCAAGCTAGTGTTGAGGGCAATGGTAAATTTTTTAAGCAAAATGCGAATGGAACTTCAAGTAGTGGTAATAAAACTTTAGTATTGTGA", "aa": "MVNGGLPSVTLTFEQNREQAKTRQRKRQVDAQVRRNAISSVEHNYQLSLETTDGVVLFDSLDNNFINASIRAVVPQNTSVNSALTFEYKLNKNVVTKESLHTDFLDFINSKNLSSSDYNTGNGQASVEGNGKFFKQNANGTSSSGNKTLVL", "aa_hexdigest": "b97b2bf3ac8d845a7bbce7e6cb5459ff", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16649.161899999985, "isoelectric_point": 8.961830711364748 }, "id": "KMADJIBOEG_637", "locus": "GCA000292445_00616" }, { "type": "cds", "sequence": "contig_1", "start": 337451, "stop": 337759, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGGTGTTATTTTTGGGAGTTACAAACTGTTAAATGACAGCAGGTTAGGTGCTGTTTTCAACGGTTCTACCACTACAACTGTTTACTTTTTAAACCATAAGTCAACTAACAATACCTCATTAGATCCTCAGCAAACAAAACCAACCAACGGGATTGAAAACATCACTAACATTGATAGCTTTTTAGATGGTGTTGAAAAAAGTTATGCTAACAGTTTATTCATTCAGGGCTTTTCTTCTGTAAACATTACTAAAAACACTAATGATAAAACTGCTAGTGAACTTGACAACATTGACAAGTCCTGA", "aa": "MFGVIFGSYKLLNDSRLGAVFNGSTTTTVYFLNHKSTNNTSLDPQQTKPTNGIENITNIDSFLDGVEKSYANSLFIQGFSSVNITKNTNDKTASELDNIDKS", "aa_hexdigest": "227633aa5314c59c3396f6de1da7e28e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11178.170399999988, "isoelectric_point": 5.107802009582519 }, "id": "KMADJIBOEG_638", "locus": "GCA000292445_00617" }, { "type": "cds", "sequence": "contig_1", "start": 337877, "stop": 339265, "strand": "+", "frame": 2, "gene": "spoT", "product": "putative guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase", "db_xrefs": [ "COG:COG0317", "COG:TK", "EC:3.1.7.2", "GO:0008893", "GO:0015970", "SO:0001217", "UniRef:UniRef50_P75386", "UniRef:UniRef90_P47520" ], "nt": "ATGGCAACCATTCAGGAAATTGAGTGTGATTTTTTAGCTAAAATAGCACAAAAATTTACTAATGCAGAGATTGAATTAATTAACAAAGCATTCTATCACGCTAAAACTTGGCATGAAAACCAGAAACGGCTTAGCGGTGAACCTTTTTTTATCCATCCTTTAAGAACGGCATTATCACTAGTTGAATGGAACATGGATCCTATCACTATTTGTGCTGGTTTGTTACATGACATCATTGAAGATACAGACCAAACCGAAGCTAATATAGCAATGATTTTTAACAAAGAAATTGCTGAGCTTGTCACTAAGGTTACAAAGATTACCAATGAATCTAAAAAGCAACGTCATCTCAAAAATAAAAAGGAGAATCTTAACTTAAAAAGCTTTGTTAACATTGCAATCAATTCTCAACAAGAGATAAATGTAATGGTACTAAAACTAGCAGATCGACTTGATAACATCGCTTCCATTGAGTTTCTCCCCATTGAAAAGCAAAAGGTAATTGCAAAAGAAACTTTAGAACTTTATGCAAAGATTGCTGGGAGGATTGGGATGTATCCTGTTAAAACAAAATTAGCAGATCTTTCATTTAAGGTGTTGGATTTAAAAAACTATGATAACACCCTGTCAAAGATTAACAAGCAAAAGGTCTTTTATGACAATGAGTGGGATAACTTCAAACAACAATTAAAAAAAATCTTAGCGCAAAATCAGATAGAATACCAACTTGAAAGTCGGATTAAAGGCATTTACTCTACATATAAAAAACTAACTGTTCATGAACAGAACATCAGTAAGATCCATGATCTTTTTGCTATCCGCTTAATTACTAAATCAGAACTTGATTGTTATCACATCCTTGGTTTAATTCACCTTAATTTTTTAATTGACAGTAAATACTTCAAAGACTATATTGCCTCACCTAAACAAAACCTTTACCAATCAATTCATACCACTGTTCGTTTAAAAGGGTTAAATGTTGAGATCCAAATTAGAACCCAACAGATGGACAATGTTAGTAAGTTTGGCTTAGCTAGTCACTGGATCTACAAAGAACAGAAAGAGGGATTGTTAGCACCTGCTTTGCAACTTAATTACCTAGTGACAAAACAAAAACACTCACATGATTTTCTAAAAAGGATTTTTGGGACTGATATTATCAAGATTAATGTTAGTGCTAGTCATGAACCTAATGTAATTAAGCAAATTAATGTTGATAGCAACAATAAACTCCTTGATATTGCTTTTGAAAACTATCCCAAGCAATTTGCTAAATTAACCAAAATTGAAATTGATGGGGTTGAGATCAATTCTTTTGATACTAGTGTTGAAAATGAGATGCTGATTGAATTTTACTTTGGCAAGAATAACAATTTGAAATCAAAGTGA", "aa": "MATIQEIECDFLAKIAQKFTNAEIELINKAFYHAKTWHENQKRLSGEPFFIHPLRTALSLVEWNMDPITICAGLLHDIIEDTDQTEANIAMIFNKEIAELVTKVTKITNESKKQRHLKNKKENLNLKSFVNIAINSQQEINVMVLKLADRLDNIASIEFLPIEKQKVIAKETLELYAKIAGRIGMYPVKTKLADLSFKVLDLKNYDNTLSKINKQKVFYDNEWDNFKQQLKKILAQNQIEYQLESRIKGIYSTYKKLTVHEQNISKIHDLFAIRLITKSELDCYHILGLIHLNFLIDSKYFKDYIASPKQNLYQSIHTTVRLKGLNVEIQIRTQQMDNVSKFGLASHWIYKEQKEGLLAPALQLNYLVTKQKHSHDFLKRIFGTDIIKINVSASHEPNVIKQINVDSNNKLLDIAFENYPKQFAKLTKIEIDGVEINSFDTSVENEMLIEFYFGKNNNLKSK", "aa_hexdigest": "754fe49611551e69713014197dd74112", "start_type": "ATG", "rbs_motif": "TTATA", "genes": [ "spoT" ], "truncated": "3-prime", "pseudogene": { "start": 337877, "stop": 339568, "observations": { "mutation": [ 339263, 339530 ], "stop": [ 339263, 339530 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47520", "query_cov": 1.0, "subject_cov": 0.6416666666666667, "identity": 0.998, "score": 894.0, "evalue": 0.0, "gene_start": 1, "gene_end": 462, "reference_start": 1, "reference_end": 462, "reference_sequence": "MATIQEIECDFLAKIAQKFTNAEIELINKAFYHAKTWHENQKRLSGEPFFIHPLRTALSLVEWNMDPITICAGLLHDIIEDTDQTEANIAMIFSKEIAELVTKVTKITNESKKQRHLKNKKENLNLKSFVNIAINSQQEINVMVLKLADRLDNIASIEFLPIEKQKVIAKETLELYAKIAGRIGMYPVKTKLADLSFKVLDLKNYDNTLSKINKQKVFYDNEWDNFKQQLKKILAQNQIEYQLESRIKGIYSTYKKLTVHEQNISKIHDLFAIRLITKSELDCYHILGLIHLNFLIDSKYFKDYIASPKQNLYQSIHTTVRLKGLNVEIQIRTQQMDNVSKFGLASHWIYKEQKEGLLAPALQLNYLVTKQKHSHDFLKRIFGTDIIKINVSASHEPNVIKQINVDSNNKLLDIAFENYPKQFAKLTKIEIDGVEINSFDTSVENEMLIEFYFGKNNNLKSKWIRYMNNPIYREKVKKSLAKLAKSGRYSELAFYEKELGEKQLKLASETEIQKRLNTLRIKKMSDYLALIECTNFTNDEHLLFLAKNNDKWNKLTKPLKFAFSKVVFHNSYFEQIEGIFITKIVIEPCCSKIPDMPEQVTGILTKNILSVHRYGCKNLQNKKQLKIIPLYWNIQQLKLKPRKFRSYININGVWSEKTINKICQTIINGDGYIEKIIPKINKQKDEFDLNITLFVNNYQQLLTLMDQITTKNISFSWKYL" }, "paralog": false, "identity": 0.9928952042628775, "score": 1098.0, "evalue": 0.0, "description": "Internal stop codon at 339263, 339530. Nonsense mutation around 339263, 339530.", "psc": { "uniref90_id": "UniRef90_P47520", "gene": "spoT", "product": "Probable guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase", "ec_ids": [ "3.1.7.2" ], "uniref50_id": "UniRef50_P75386", "cog_id": "COG0317", "cog_category": "TK", "go_ids": [ "GO:0008893", "GO:0015970" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47520", "EC:3.1.7.2", "UniRef:UniRef50_P75386", "COG:COG0317", "COG:TK", "GO:0008893", "GO:0015970" ] }, "pscc": { "uniref50_id": "UniRef50_P75386", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75386" ], "product": "Probable guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase" } }, "id": "KMADJIBOEG_639", "locus": "GCA000292445_00618" }, { "type": "cds", "sequence": "contig_1", "start": 339275, "stop": 339532, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAACCCTATATACCGTGAAAAGGTAAAAAAGAGCTTGGCTAAACTAGCTAAATCTGGTAGATACAGTGAGTTAGCTTCTTATGAAAAAGAACTGGGTGAAAAACAGTTAAAACTTGCTAGTGAAACTGAAATCCAAAAACGCTTAAACACCCTAAGAATTAAAAAAATGAGTGATTACTTAGCGTTAATTGAGTGTACTAACTTTACTAATGATGAACATTTGTTGTTTCTAGCTAAAAACAACGACAAGTGA", "aa": "MNNPIYREKVKKSLAKLAKSGRYSELASYEKELGEKQLKLASETEIQKRLNTLRIKKMSDYLALIECTNFTNDEHLLFLAKNNDK", "aa_hexdigest": "20724908d7aeebe3e00081759b8b6431", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9908.352199999987, "isoelectric_point": 9.380037117004395 }, "id": "KMADJIBOEG_640", "locus": "GCA000292445_00619" }, { "type": "cds", "sequence": "contig_1", "start": 339629, "stop": 339772, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTGAACCATGTTGTAGTAAGATCCCTGATATGCCTGAACAAGTAACTGGTATCTTAACTAAAAACATTTTAAGTGTTCACCGTTATGGTTGTAAGAATTTACAAAATAAAAAGCAGTTAAAAATTATCCCGTTATATTGA", "aa": "MIEPCCSKIPDMPEQVTGILTKNILSVHRYGCKNLQNKKQLKIIPLY", "aa_hexdigest": "705a2ebcf5d94b3fa38f79fd7e531505", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5400.473400000001, "isoelectric_point": 9.21854381561279 }, "id": "KMADJIBOEG_641", "locus": "GCA000292445_00620" }, { "type": "cds", "sequence": "contig_1", "start": 339833, "stop": 340027, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTGGAGTGAAAAAACCATTAATAAAATCTGTCAAACAATTATTAATGGTGATGGTTATATTGAAAAAATAATTCCCAAGATCAACAAACAAAAAGATGAATTTGATTTAAACATCACCCTTTTTGTTAATAACTACCAACAACTTCTCACCTTAATGGACCAAATTACCACTAAGAATATCAGCTTTAGTTGA", "aa": "MWSEKTINKICQTIINGDGYIEKIIPKINKQKDEFDLNITLFVNNYQQLLTLMDQITTKNISFS", "aa_hexdigest": "6eee32e0da4f977d666e149445b966b7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7510.615500000003, "isoelectric_point": 6.07798671722412 }, "id": "KMADJIBOEG_642", "locus": "GCA000292445_00621" }, { "type": "cds", "sequence": "contig_1", "start": 340036, "stop": 340692, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MG279", "db_xrefs": [ "GO:0005886", "RefSeq:WP_014894253.1", "SO:0001217", "UniParc:UPI00027B3E32", "UniRef:UniRef100_UPI00027B3E32", "UniRef:UniRef50_P47521", "UniRef:UniRef90_P47521" ], "nt": "ATGATTAGACTATTAAAAAAACTTGCAGTTTTTTTTATTATTTTGGTAGGAATTTTATTGTTAGGTGGAATTGCTACTGCTGGTTATTTTGCTTTTACTTACCGTGAACCTATTAACAACTACTATAAAGAGGGATATAACAAGATTAGTGAATATAACACTGAAATTAAAAAGATCTCCCAAAATATCTTCCAAAATAACCTAGTTAAAACACTTTCAGAAGTTGAAAAATCTTTAAACGAAGGGCGAAAATTAACGCAAAACAATTCATTTGCATCAGGGTTAGATAGTTCTTTAAATGCACTTGAAGGTTCATTGAAAAAAATTAATAACTTTGATAGTAATGCTGCTTTTACCCAAATTAAACACACATTAAATAACATAACTAGCTTTGTTGATCAAATGCTAGAAAAGTTCCCAAATCCAAACCAAAATGATGATTTTAAGCGGTATTTAACTGAAGTTTCCCAAATCCTTTTCTATACAGGAATCTCAATTATTGGGGCATTTTTTGTTTCTGGTTTTTTATTAATTTTATTTACAAAGAAAGTTTATGGAGTGAGAGTATCACGTTTCAATCCACAACGGCTACTAAAAAAGCACTTAGTTTTACTTTTAAGAGATGAAGAAGTGTATGATGCGGTGTTTGGTAACTAA", "aa": "MIRLLKKLAVFFIILVGILLLGGIATAGYFAFTYREPINNYYKEGYNKISEYNTEIKKISQNIFQNNLVKTLSEVEKSLNEGRKLTQNNSFASGLDSSLNALEGSLKKINNFDSNAAFTQIKHTLNNITSFVDQMLEKFPNPNQNDDFKRYLTEVSQILFYTGISIIGAFFVSGFLLILFTKKVYGVRVSRFNPQRLLKKHLVLLLRDEEVYDAVFGN", "aa_hexdigest": "15286bb6a7e2b53f34d4fd91ef46664d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3E32", "ncbi_nrp_id": "WP_014894253.1", "uniref100_id": "UniRef100_UPI00027B3E32", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3E32", "RefSeq:WP_014894253.1", "UniRef:UniRef100_UPI00027B3E32" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3E32", "uniref90_id": "UniRef90_P47521" }, "psc": { "uniref90_id": "UniRef90_P47521", "product": "Uncharacterized protein MG279", "uniref50_id": "UniRef50_P47521", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47521", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47521" ], "product": "Uncharacterized protein MG279" }, "genes": [], "id": "KMADJIBOEG_643", "locus": "GCA000292445_00622" }, { "type": "cds", "sequence": "contig_1", "start": 340685, "stop": 341482, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MG280", "db_xrefs": [ "GO:0005886", "RefSeq:WP_014894254.1", "SO:0001217", "UniParc:UPI00027B3E33", "UniRef:UniRef100_UPI00027B3E33", "UniRef:UniRef50_P47522", "UniRef:UniRef90_P47522" ], "nt": "ATGGTTATTTTTATTAATCGGTTTTCGAAAACCATCTTGCTTTTCTTTGGAATGTTGGTTTTTGTATTTCTGTTAGGGTTTGGTATTACTGCTTTGTATTTTCGTAGTACAGCAGCTAACCTTTATGTTCAAGCTCGTAATTCCATTGATAGTTCTTTCAATTCTGCTAAAGCCTTTGCTAATGCTTTAGCAAATAGTGCTAACCAATTTAGCAAAAGTTCCATTACTAACAATTTAGATCAAGTTAAAAAAGACTTAGAGCAATCTTTACAAAAAGTAGATGAATACAAAAAGAACTTAGAGAGTCAGAACAACCTAGGTAATATTAGTCAAGAAAAGATTAGAGAACTTGATGCAACCAAAAAAGACTTGGAAAACAGTAAAACACAATTAGAAAACTTTAAAAGTAATCTTGATAAAAATGGTACTGTTAGTTCATCTCCATCTGTAAAAAAACAAGCAACCACTGATGGTGTAATTAGTGCAGTTAGTGAGTTTTCTACACAAGCACAAAGCATTGTTAGTAGCTATGAAAAGATCAAAAACAACATCCCTTCTTCTGAACAGTTCAATAATTATTATGATGTCACAATGATAACCATTGTTGCTGTATCAGGTGGGATGCTAGCAATTTTGATTACAACCATTGTCTTTTCCTTTTTAACTTCTAAAAAAAGGGGATTAATCAGGTTTTCAAGATTTACTAGTACTGAACAGTTAGCTGATCATGTTAATGATATCTTAGATCGTTATCCTGATTTGGAAGAGGAAGTGATAACAGCATTAGACCATGATTAG", "aa": "MVIFINRFSKTILLFFGMLVFVFLLGFGITALYFRSTAANLYVQARNSIDSSFNSAKAFANALANSANQFSKSSITNNLDQVKKDLEQSLQKVDEYKKNLESQNNLGNISQEKIRELDATKKDLENSKTQLENFKSNLDKNGTVSSSPSVKKQATTDGVISAVSEFSTQAQSIVSSYEKIKNNIPSSEQFNNYYDVTMITIVAVSGGMLAILITTIVFSFLTSKKRGLIRFSRFTSTEQLADHVNDILDRYPDLEEEVITALDHD", "aa_hexdigest": "01e7b1698599e27655335a45ecc69472", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3E33", "ncbi_nrp_id": "WP_014894254.1", "uniref100_id": "UniRef100_UPI00027B3E33", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3E33", "RefSeq:WP_014894254.1", "UniRef:UniRef100_UPI00027B3E33" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3E33", "uniref90_id": "UniRef90_P47522" }, "psc": { "uniref90_id": "UniRef90_P47522", "product": "Uncharacterized protein MG280", "uniref50_id": "UniRef50_P47522", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47522", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47522" ], "product": "Uncharacterized protein MG280" }, "genes": [], "id": "KMADJIBOEG_644", "locus": "GCA000292445_00623" }, { "type": "cds", "sequence": "contig_1", "start": 341543, "stop": 342373, "strand": "-", "frame": 3, "gene": null, "product": "IgG-blocking protein M", "db_xrefs": [ "GO:0005515", "GO:0016020", "SO:0001217", "UniRef:UniRef50_P47523", "UniRef:UniRef90_P47523" ], "nt": "TTGCAATTACCAGAATCAGTTAAAAAAGTTTCACTTTATGGTGATTACACTGGTGTTAATGTAGCTAAACAAATCTTTGCAAACGTAGTTGAGTTGGAATTTTATTCCACCAGTAAAGCAAATAGTTTTGGTTTTAACCCATTAGTATTGGGAAGTAAAACTAATGTTATCTATGATCTGTTTGCCTCAAAACCATTTACCCACATTGATTTAACCCAAGTTACACTCCAAAACAGTGATAATTCAGCCATTGATGCTAATAAGCTCAAACAAGCAGTTGGTGATATTTACAACTACAGAAGGTTTGAAAGACAGTTCCAAGGTTATTTTGCTGGGGGATACATTGACAAGTACTTAGTTAAGAATGTTAATACCAACAAGGATAGTGATGATGATTTGGTGTACCGTTCTTTAAAAGAACTTAACCTCCACTTAGAAGAAGCTTACAGAGAAGGAGATAACACCTATTACAGGGTTAATGAAAACTATTACCCTGGTGCTAGCATCTATGAAAATGAACGTGCAAGCAGAGACAGCGAGTTTCAAAATGAAATTTTAAAGCGTGCTGAACAAAACGGAGTAACATTTGATGAAAACATCAAAAGAATTACTGCTAGTGGTAAATACAGTGTACAGTTCCAAAAACTAGAAAATGATACAGATAGTAGTCTTGAAAGAATGACTAAAGCAGTTGAAGGGTTGGTTACTGTAATTGGTGAAGAAAAGTTTGAAACAGTTGATATTACTGGTGTTTCCAGTGATACAAATGAAGTTAAATCACTAGCAAAAGAACTTAAAACCAACGCATTGGGTGTTAAACTTAAACTCTAG", "aa": "MQLPESVKKVSLYGDYTGVNVAKQIFANVVELEFYSTSKANSFGFNPLVLGSKTNVIYDLFASKPFTHIDLTQVTLQNSDNSAIDANKLKQAVGDIYNYRRFERQFQGYFAGGYIDKYLVKNVNTNKDSDDDLVYRSLKELNLHLEEAYREGDNTYYRVNENYYPGASIYENERASRDSEFQNEILKRAEQNGVTFDENIKRITASGKYSVQFQKLENDTDSSLERMTKAVEGLVTVIGEEKFETVDITGVSSDTNEVKSLAKELKTNALGVKLKL", "aa_hexdigest": "2832ab3b78cfd7c2e74be53a2d48d8a9", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 341543, "stop": 342673, "observations": { "mutation": [ 342559 ], "stop": [ 342559 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47523", "query_cov": 1.0, "subject_cov": 0.49640287769784175, "identity": 0.996, "score": 535.0, "evalue": 1.6e-187, "gene_start": 1, "gene_end": 276, "reference_start": 281, "reference_end": 556, "reference_sequence": "MQFKKHKNSVKFKRKLFWTIGVLGAGALTTFSAVMITNLVNQSGYALVASGRSGNLGFKLFSTQSPSAEVKLKSLSLNDGSYQSEIDLSGGANFREKFRNFANELSEAITNSPKGLDRPVPKTEISGLIKTGDNFITPSFKAGYYDHVASDGSLLSYYQSTEYFNNRVLMPILQTTNGTLMANNRGYDDVFRQVPSFSGWSNTKATTVSTSNNLTYDKWTYFAAKGSPLYDSYPNHFFEDVKTLAIDAKDISALKTTIDSEKPTYLIIRGLSGNGSQLNELQLPESVKKVSLYGDYTGVNVAKQIFANVVELEFYSTSKANSFGFNPLVLGSKTNVIYDLFASKPFTHIDLTQVTLQNSDNSAIDANKLKQAVGDIYNYRRFERQFQGYFAGGYIDKYLVKNVNTNKDSDDDLVYRSLKELNLHLEEAYREGDNTYYRVNENYYPGASIYENERASRDSEFQNEILKRAEQNGVTFDENIKRITASGKYSVQFQKLENDTDSSLERMTKAVEGLVTVIGEEKFETVDITGVSSDTNEVKSLAKELKTNALGVKLKL" }, "paralog": false, "identity": 0.9973404255319149, "score": 741.0, "evalue": 1.87e-271, "description": "Internal stop codon at 342559. Nonsense mutation around 342559.", "psc": { "uniref90_id": "UniRef90_P47523", "product": "IgG-blocking protein M", "uniref50_id": "UniRef50_P47523", "go_ids": [ "GO:0005515", "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47523", "UniRef:UniRef50_P47523", "GO:0005515", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47523", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47523" ], "product": "Uncharacterized protein MG281" } }, "id": "KMADJIBOEG_645", "locus": "GCA000292445_00624" }, { "type": "cds", "sequence": "contig_1", "start": 342557, "stop": 343213, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF20757.2" ], "nt": "ATGCAATTTAAAAAACATAAGAATAGTGTGAAATTTAAGCGTAAATTATTTTGGACTATTGGTGTTTTAGGTGCTGGAGCGTTAACCACATTCTCAGCAGTTATGATCACTAACCTAGTTAATCAAAGTGGTTATGCTTTGGTTGCATCAGGTAGGAGTGGTAACCTCGGTTTTAAGTTGTTCTCAACACAATCTTCTAGTGCAGAAGTAAAGTTGAAATCACTTTCTTTAAACGATGGTAGCTATCAGAGTGAGATTGATTTATCTGGTGGTGCTAACTTCCGTGAGAAATTTAGAAACTTTGCTAATGAGTTATCAGAAGCGATCACTAACAGTCCAAAGGGTTTAGACCGTCCTGTTCCTAAAACTGAAATTAGTGGCTTAATTAAAACAGGTGATAACTTTATTACTCCTAGTTTTAAAGCTGGTTATTATGATCATGTTGCTAGTGATGGTTCACTGCTTAGCTATTACCAATCAACTGAATATTTCAATAACCGCGTGTTGATGCCAATCTTGCAAACAACTAACGGTACTCTCATGGCAAACAACCGTGGTTATGATGATGTGTTTAGACAAGTTCCTAGTTTCAGCGGATGGAGTAACACAAAAGCAACAACTGTTTCAACTAGTAATAACCTTACTTATGATAAGTGA", "aa": "MQFKKHKNSVKFKRKLFWTIGVLGAGALTTFSAVMITNLVNQSGYALVASGRSGNLGFKLFSTQSSSAEVKLKSLSLNDGSYQSEIDLSGGANFREKFRNFANELSEAITNSPKGLDRPVPKTEISGLIKTGDNFITPSFKAGYYDHVASDGSLLSYYQSTEYFNNRVLMPILQTTNGTLMANNRGYDDVFRQVPSFSGWSNTKATTVSTSNNLTYDK", "aa_hexdigest": "897f744a6d71f2b20feac6213d483cce", "start_type": "ATG", "rbs_motif": "TTCTA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF20757.2", "name": "M_large_dom", "length": 147, "aa_cov": 0.6422018348623854, "hmm_cov": 1.0, "evalue": 2.381461895808988e-85, "score": 281.8677062988281, "start": 79, "stop": 218 } ], "seq_stats": { "molecular_weight": 23971.6373, "isoelectric_point": 9.587431907653805 }, "id": "KMADJIBOEG_646", "locus": "GCA000292445_00625" }, { "type": "tRNA", "sequence": "contig_1", "start": 343394, "stop": 343467, "strand": "-", "gene": "trnG", "product": "tRNA-Gly(tcc)", "amino_acid": "Gly", "anti_codon": "tcc", "score": 73.6, "nt": "GCGAGTATAGTTTAATGGTAGAACATCAGCCTTCCAAGCTGATCGTGTCGGTTCGATTCCGATTACTCGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 343433, 343435 ], "id": "KMADJIBOEG_30", "locus": "GCA000292445_00626" }, { "type": "tRNA", "sequence": "contig_1", "start": 343475, "stop": 343560, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(taa)", "amino_acid": "Leu", "anti_codon": "taa", "score": 72.3, "nt": "GCCCAAGTGGCGGAATGGTAGACGCATGGGATTTAAGATCCCACGCCAGTAATGGTGTGCCGGTTCAAGTCCGGCTTTGGGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 343525, 343527 ], "id": "KMADJIBOEG_29", "locus": "GCA000292445_00627" }, { "type": "tRNA", "sequence": "contig_1", "start": 343561, "stop": 343635, "strand": "-", "gene": "trnK", "product": "tRNA-Lys(ttt)", "amino_acid": "Lys", "anti_codon": "ttt", "score": 74.0, "nt": "GACTCACTAGCTCAGCGGTAGAGCATTTGACTTTTAATCAAAGGGTCCCGAGTTCGATCCTCGGGTGGGTCACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 343608, 343610 ], "id": "KMADJIBOEG_28", "locus": "GCA000292445_00628" }, { "type": "tRNA", "sequence": "contig_1", "start": 343682, "stop": 343756, "strand": "-", "gene": "trnQ", "product": "tRNA-Gln(ttg)", "amino_acid": "Gln", "anti_codon": "ttg", "score": 70.6, "nt": "TGGGATGTAGCCAAGCGGTAAGGCAATAGACTTTGACTCTATCATGCGATGGTTCGATCCCATCCATCCCAGCCA", "db_xrefs": [ "SO:0000261" ], "anti_codon_pos": [ 343722, 343724 ], "id": "KMADJIBOEG_27", "locus": "GCA000292445_00629" }, { "type": "tRNA", "sequence": "contig_1", "start": 343761, "stop": 343847, "strand": "-", "gene": "trnY", "product": "tRNA-Tyr(gta)", "amino_acid": "Tyr", "anti_codon": "gta", "score": 72.6, "nt": "GGACAGGTAGCGAAGTGGCTAAACGCTTCTGACTGTAGATCAGACACCTTTATGGTTTCGGGAGTTCGAATCTCTCCCTGTCCACCA", "db_xrefs": [ "SO:0000272" ], "anti_codon_pos": [ 343839, 343841 ], "id": "KMADJIBOEG_26", "locus": "GCA000292445_00630" }, { "type": "cds", "sequence": "contig_1", "start": 343875, "stop": 344360, "strand": "-", "frame": 2, "gene": "greA", "product": "transcription elongation factor GreA", "db_xrefs": [ "COG:COG0782", "COG:K", "GO:0003677", "GO:0006354", "GO:0032784", "GO:0070063", "RefSeq:WP_009885993.1", "SO:0001217", "UniParc:UPI000012BAA4", "UniRef:UniRef100_P47524", "UniRef:UniRef50_P47524", "UniRef:UniRef90_P47524" ], "nt": "ATGGAACTAAATAAAAATTACCTAACTCAAGAAGGATTTAAGCAACTGGAAAAAGAACTTGAAAACCTAATCCAAGTTAAACGTCCTGAGATTATCAGACTCTTACAAGAAGCACGTGATCAGGGTGATTTAAGTGAAAATGCTGATTATGATGCAGCTAAAGCACAGCAAGGTGAGATTGAAACTAGGATTGCTGAAATTCAAGATATATTAGCCAACGCTAAGTTAATTAGTGACCACCAAGCAAAAACCAAAGTAACAAAAGTTAGCTTAGGGAGCACTGTTGAGATCTATGATTACAGTTCTAAATCCAACGAAAAATACACAATTGTAGGTACACTTGAAGCAAATCCTGAAGAACACAAAATTTCCAATGAATCACCCCTTGCCCTTGCAATCTATGGGCGTTTAATTGGTGATGAATGTGATGTTGTTGGTATTGAAGTTCCCTATCGTGTTAAAATCCTGAAGATCAGCAACAGATAA", "aa": "MELNKNYLTQEGFKQLEKELENLIQVKRPEIIRLLQEARDQGDLSENADYDAAKAQQGEIETRIAEIQDILANAKLISDHQAKTKVTKVSLGSTVEIYDYSSKSNEKYTIVGTLEANPEEHKISNESPLALAIYGRLIGDECDVVGIEVPYRVKILKISNR", "aa_hexdigest": "d2968165f7cac4163cfd0cda1e5c4990", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012BAA4", "ncbi_nrp_id": "WP_009885993.1", "uniref100_id": "UniRef100_P47524", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012BAA4", "RefSeq:WP_009885993.1", "UniRef:UniRef100_P47524" ] }, "ips": { "uniref100_id": "UniRef100_P47524", "uniref90_id": "UniRef90_P47524" }, "psc": { "uniref90_id": "UniRef90_P47524", "gene": "greA", "product": "transcription elongation factor GreA", "uniref50_id": "UniRef50_P47524", "cog_id": "COG0782", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0006354", "GO:0032784", "GO:0070063" ] }, "pscc": { "uniref50_id": "UniRef50_P47524", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47524" ], "product": "Transcription elongation factor GreA" }, "genes": [ "greA" ], "id": "KMADJIBOEG_647", "locus": "GCA000292445_00631" }, { "type": "cds", "sequence": "contig_1", "start": 344541, "stop": 344675, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W775" ], "nt": "ATGAGAGAAAACAATAGTAATGCTCAAAACAAAAAGAAGATCCGCTTTTTAGCTTTGGGCATCATTATTCTAATCGTACTAATTGCTTTAATTACTATGCTTTTTATCACTGGAGTAATTAGTCGCAACCGTTAA", "aa": "MRENNSNAQNKKKIRFLALGIIILIVLIALITMLFITGVISRNR", "aa_hexdigest": "37053122028846411abf77e2ea861a2f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB38W775", "query_cov": 0.9090909090909091, "subject_cov": 0.8695652173913043, "identity": 0.65, "score": 49.3, "evalue": 5.65e-07, "valid": false, "uniref50_id": "UniRef50_A0AB38W775" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W775", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W775" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5011.0954999999985, "isoelectric_point": 11.999967765808105 }, "id": "KMADJIBOEG_648", "locus": "GCA000292445_00632" }, { "type": "cds", "sequence": "contig_1", "start": 344815, "stop": 345141, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAATCCAACAGCTTTACAATGATGCAATTGCAAATAAAAATCAATTGTCTGCAATTGCTCTAACTAAATTCCAACCAACTACTAGTTTTTGTTATCAAGTATTCCAAGTACAACTCCCTACCCTTTCTTTTTACAGTGAATATCAAAAGGAAAAAACCCATATCAAAGGTTTTAATCCTGAGCTTTTTTTAATTAATCAAGTTGGTCAAAAACAACTCAATGATCCTTTGGTTTTACGACCTACTAGTGAGATTGCTTTTTGCAACTTATGGAAAAAACAAGAGTTATCTTACCATGATCTACCTTTAATTTATAACCAGTGA", "aa": "MEIQQLYNDAIANKNQLSAIALTKFQPTTSFCYQVFQVQLPTLSFYSEYQKEKTHIKGFNPELFLINQVGQKQLNDPLVLRPTSEIAFCNLWKKQELSYHDLPLIYNQ", "aa_hexdigest": "82917a68bea6990deb1af9ee76d9273a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12659.35619999999, "isoelectric_point": 6.70810375213623 }, "id": "KMADJIBOEG_649", "locus": "GCA000292445_00633" }, { "type": "cds", "sequence": "contig_1", "start": 345322, "stop": 346143, "strand": "+", "frame": 1, "gene": "proS", "product": "proline--tRNA ligase", "db_xrefs": [ "COG:COG0442", "COG:J", "EC:6.1.1.15", "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433", "GO:0017101", "KEGG:K01881", "SO:0001217", "UniRef:UniRef50_P47525", "UniRef:UniRef90_P47525" ], "nt": "TTGAAAAGTGAAAGTGAAAAATTTGCAGGTGCTAAAAACACATGGACAATAGAAGCAATTATGCCTGATGGACAAAGTTTGCAATGTGCCACTAGCCATGATTTAGGTGACACTTTTACAAAGAGTTTTACTATCAGCTATCAGAGTAAAACTAACCAAAAAATGACTCCAAGTAGTTTTAGTTGTGGGATGTCAACTAGGATCTTAGGAGCAATTTTTTTAACCCACAGCGATGATTATGGTTTGGTTTTACCTTGGTATCTAGCAAGTAAACAAGTCAAGTTATACCTGTTTGATAAAAACAATAACCCTAAAACAAGAGCTTTAGCTTTTTTAGTGAAGGATTTTTTAGAAAAACTCAAAATTCGCTTTAGTTTTATAGAAATTAACAATCAACTAGGTAAACAACTTTTAAAAGGAGAAATAGAAGGTATTCCATTACAGATGATTGTTGATAATGAAAAAACTATTAACATCTTCAACCGCTTAACACGTTTAAAAACCAGCTTAACATTTGCAAATCTCCAAACTGAATTTGTTAATTTAGTTAACAACTACCATACAGAGATGTATAGAAAAGCAAATGATTTAGTTGAACAAAAACTAGCAAGAGTACAAACTTTAAAGGAAATTGAACAAGCATTCAAAAATAAAAAGGCTGTTTTATGTACCGTGAAGTTAACTGGTGAACTTGAACAACACTTAAAGACAAAATACCAAGTTAGTGTTAGGTGTGTTTTTAAAAAGTCAGATGTAACACAAAACTGTCCTTTTACAAATCAACCTTGTTTTGATTCAGTTTTAATTGCACGTGCTTACTAA", "aa": "MKSESEKFAGAKNTWTIEAIMPDGQSLQCATSHDLGDTFTKSFTISYQSKTNQKMTPSSFSCGMSTRILGAIFLTHSDDYGLVLPWYLASKQVKLYLFDKNNNPKTRALAFLVKDFLEKLKIRFSFIEINNQLGKQLLKGEIEGIPLQMIVDNEKTINIFNRLTRLKTSLTFANLQTEFVNLVNNYHTEMYRKANDLVEQKLARVQTLKEIEQAFKNKKAVLCTVKLTGELEQHLKTKYQVSVRCVFKKSDVTQNCPFTNQPCFDSVLIARAY", "aa_hexdigest": "30b484fd8dcc2c15dd577c282f1d0ae7", "start_type": "TTG", "rbs_motif": null, "genes": [ "proS" ], "truncated": "5-prime", "pseudogene": { "start": 345022, "stop": 346143, "observations": { "mutation": [ 345139, 345202, 345265 ], "stop": [ 345139, 345202, 345265 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47525", "query_cov": 1.0, "subject_cov": 0.5652173913043478, "identity": 0.996, "score": 536.0, "evalue": 2.84e-189, "gene_start": 1, "gene_end": 273, "reference_start": 211, "reference_end": 483, "reference_sequence": "MTKKTQDLTSWYDQLLVKAKLICHGEVKGTVCFLNNSWGLWMEIQQLYNDAIANKNQLSAIALTKFQPTTSFCYQVFQVQLPTLSFYSEYQKEKTHIKGFNPELFLINQVGQKQLNDPLVLRPTSEIAFCNLWKKQELSYHDLPLIYNQWTQVFRAEKNTRPFLRNSEFYWQETHGLFVDQSQSEQAAISFWNLYQDLIINKLCIPAFVGLKSESEKFAGAKNTWTIEAIMPDGQSLQCATSHDLGDTFTKSFTISYQSKTNQKMTPSSFSCGMSTRILGAIFLTHSDDYGLVLPWYLASKQVKLYLFDKNNNPKTRALAFLVKDFLEKLKIRFSFIEINNQLGKQLLKGEIEGIPLQMIVDNEKTINIFNRLTRLKTSLTFANLQTEFVNLVNNYHTEMYRKANDLVEQKLARVQTLKEIEQAFKNKKAVLCTVKLTGELEQHLKTKYQVSVRCVFKKSDVTQNCPFTNQPCFDSVLIARAY" }, "paralog": false, "identity": 0.9919571045576407, "score": 738.0, "evalue": 3.8e-271, "description": "Internal stop codon at 345139, 345202, 345265. Nonsense mutation around 345139, 345202, 345265.", "psc": { "uniref90_id": "UniRef90_P47525", "gene": "proS", "product": "proline--tRNA ligase", "ec_ids": [ "6.1.1.15" ], "uniref50_id": "UniRef50_P47525", "cog_id": "COG0442", "cog_category": "J", "kegg_orthology_id": "K01881", "go_ids": [ "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433", "GO:0017101" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47525", "EC:6.1.1.15", "UniRef:UniRef50_P47525", "COG:COG0442", "COG:J", "KEGG:K01881", "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433", "GO:0017101" ] }, "pscc": { "uniref50_id": "UniRef50_P47525", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47525" ], "product": "Proline--tRNA ligase" } }, "id": "KMADJIBOEG_650", "locus": "GCA000292445_00634" }, { "type": "cds", "sequence": "contig_1", "start": 346550, "stop": 346690, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAAAAAGAAACTGTTGATTTTATCTATGACATCTATATTTATGGCAAAAAGCCGTGTGATATTGGTTATTCTAATTCCACCTATTACAAAAAACTAAACAAAGCAGCTAATAGTTTTTTTGACCATTTTGTTTGA", "aa": "MLKKETVDFIYDIYIYGKKPCDIGYSNSTYYKKLNKAANSFFDHFV", "aa_hexdigest": "f2deb3d58c90f77abbe22626051eb867", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5480.226300000001, "isoelectric_point": 8.734063911437985 }, "id": "KMADJIBOEG_651", "locus": "GCA000292445_00635" }, { "type": "cds", "sequence": "contig_1", "start": 346725, "stop": 346958, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGCAATTCTCAACGTACAACGAATTCAAAATAACCAAGTCACTGAGTACACAATGAGTCCAGTGCGTAATTTTGCAAACACAAAAGATGTATATTTTGATGCGCAATTAACAAATATAGAAAGCAAAATTGATAGTAGTAGGGCTCAAATTCACCTTACTATTGCACTAAAATACAACACTAATCTCCCTGATAATATCTTTCAAGCCCACTTCAGTTTAGGCAATTGA", "aa": "MAAILNVQRIQNNQVTEYTMSPVRNFANTKDVYFDAQLTNIESKIDSSRAQIHLTIALKYNTNLPDNIFQAHFSLGN", "aa_hexdigest": "6d6c6b0bfab9efe4f3dd4b95d4f5effe", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8772.763199999994, "isoelectric_point": 6.703386116027832 }, "id": "KMADJIBOEG_652", "locus": "GCA000292445_00636" }, { "type": "cds", "sequence": "contig_1", "start": 347169, "stop": 347306, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGATACTGTAGCTAGTGAATTGTATGCAACAGTAATTAAAGAAACAATCAACTTTGGCAACATGATTAAGATTAATGCACTTGATGGTAGTAAACAGCTAACTAGTAGCCAAGGAAGTTTTAAATACAGTTGA", "aa": "MFDTVASELYATVIKETINFGNMIKINALDGSKQLTSSQGSFKYS", "aa_hexdigest": "f9c6c28bf20885274471409d04788bbd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4949.565500000001, "isoelectric_point": 5.970674705505371 }, "id": "KMADJIBOEG_653", "locus": "GCA000292445_00637" }, { "type": "cds", "sequence": "contig_1", "start": 347310, "stop": 347519, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGACTATAGGAATTTAGAACAACTTGATGAGGTGAGAAACTTGATCAATATCAGTTTTGACAAACCAGTTCAAATAGTAAATGTTGATGTCAAAATTCACTATGTTCCAACTAAAGGTAGGTTACAAGAGATAAAACAACAAGGTGAGTTTGAAAATAACCTTGATGTTAATGAAAAGCTTAAACTCAATTTAATAGGCAATTGA", "aa": "MYDYRNLEQLDEVRNLINISFDKPVQIVNVDVKIHYVPTKGRLQEIKQQGEFENNLDVNEKLKLNLIGN", "aa_hexdigest": "c574042e20a6344067741eb2cd5cc493", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8157.187999999998, "isoelectric_point": 5.236087608337402 }, "id": "KMADJIBOEG_654", "locus": "GCA000292445_00638" }, { "type": "cds", "sequence": "contig_1", "start": 347610, "stop": 347768, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGGCGTTACAGTAGGTAATGATTTCTACACAATTATAGCCAACAACCAGTTTAAGTACGAAACACCTTTAGATGATCTTGAACAAAATGACTTTTTTGAGGTAAATTATTTACCTGTTTACAGTACATACAACTTTAGTGATTTAACCCAGTAA", "aa": "MIGVTVGNDFYTIIANNQFKYETPLDDLEQNDFFEVNYLPVYSTYNFSDLTQ", "aa_hexdigest": "ec53013ec041fff8439c5c6e9124735d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6108.613000000001, "isoelectric_point": 4.0500284194946286 }, "id": "KMADJIBOEG_655", "locus": "GCA000292445_00639" }, { "type": "cds", "sequence": "contig_1", "start": 347771, "stop": 348283, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MG286", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_P47528", "UniRef:UniRef90_P47528" ], "nt": "ATGATTTTTTCAATTAGTAAACGAAAGTTAATTTGCGGATTTTTGCTAGTTATTCTAACAATAGGTGGGGTTTTGGGTGGTGTTTATTTAGTTACTAAAAACAACAAGGATAACTACCAAAATGAAAGTAATTTCAACAATCAAGAACAAATTAGTAAAATCCCTAATTTCAAAGCTATTGGTCCTGAAACACAACGTATCTTAAGAGAAAGAAACTATCCTTTAGATGATAGTGGTTATTATGTTTATAAGTATGGTGAAATTAATAGGTATCTAAGAAATGAAAGTGAACTAGATGAATTGATTAACTATAGAGTGATGGTACCTTCACTTAAATCACATCACAAAAGAGTTAATTTTGATAAGGCATTTCTTGAAAGTAAATTAAGAAAATGGATTATCAAAGCAATTAAGCAACATAACTATTTCCAACACTTTGAAAATGAACCAAATTTAAGAGTTCAATATAACATGAATATTCCAGCACAAAAAATTGATGTTAATGCTGTTTGA", "aa": "MIFSISKRKLICGFLLVILTIGGVLGGVYLVTKNNKDNYQNESNFNNQEQISKIPNFKAIGPETQRILRERNYPLDDSGYYVYKYGEINRYLRNESELDELINYRVMVPSLKSHHKRVNFDKAFLESKLRKWIIKAIKQHNYFQHFENEPNLRVQYNMNIPAQKIDVNAV", "aa_hexdigest": "f448b61c050120ae22a034f1def54deb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47528", "query_cov": 1.0, "subject_cov": 0.8673469387755102, "identity": 0.9940000000000001, "score": 338.0, "evalue": 2.23e-117, "valid": true, "product": "Uncharacterized protein MG286", "uniref50_id": "UniRef50_P47528", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47528", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47528" ], "product": "Uncharacterized protein MG286" }, "genes": [], "id": "KMADJIBOEG_656", "locus": "GCA000292445_00640" }, { "type": "tRNA", "sequence": "contig_1", "start": 348637, "stop": 348711, "strand": "+", "gene": null, "product": "tRNA-Xxx", "score": 70.7, "nt": "AGGGGTATAGTTCAAAGGTAGAACATCTGTCTTCAAAATAGAGTGTTGTGGGTTCGAGTCCTGCTACCCCTGCCA", "db_xrefs": [], "id": "KMADJIBOEG_12", "locus": "GCA000292445_00641" }, { "type": "cds", "sequence": "contig_1", "start": 348873, "stop": 349127, "strand": "+", "frame": 3, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7M3VDG5", "UniRef:UniRef90_UPI0030CD7D5B" ], "nt": "TTGATCCAACTAAAAGGTTGCAAAAAGAGAGTCCAAGAAAGCGATTCAAGTAAAACAGGGGAGAAACTCACCGAAACAACTTCATCGATGAGTAGTGGTGGGGCTACATCCACTCGCAAGGCCCTCAAGGTTGAGGTGGAAAGGGGGAGTCAAAGTGATTCACTTTTAAAAAACGACTTTGCTAAAAAGCCGTTGAAACATAAGAACAATGGGGATGTGAAGTTGGATGCGAGTGGGGAGTTTGCCAAAGCCTGA", "aa": "MIQLKGCKKRVQESDSSKTGEKLTETTSSMSSGGATSTRKALKVEVERGSQSDSLLKNDFAKKPLKHKNNGDVKLDASGEFAKA", "aa_hexdigest": "3205b8aed89798266d81272b4424594f", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 348791, "stop": 349184, "observations": { "insertion": [ 348914 ], "stop": [ 349125 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0030CD7D5B", "query_cov": 0.9404761904761905, "subject_cov": 0.5984848484848485, "identity": 0.81, "score": 108.0, "evalue": 9.92e-29, "gene_start": 8, "gene_end": 84, "reference_start": 35, "reference_end": 113, "reference_sequence": "MPKVVVEYHNLNKKVVKESLEVEATDSFDPTQRLKKESPVKDSNKDSEKLEETTSSMSSGGATSTRKALKIEVERGSQSDSLLKNDFAKKPLKHKNSSGTDVKLDASGEFTKAWKPLLTTEQIKDNRGMGAT" }, "paralog": false, "identity": 0.7878787878787878, "score": 194.0, "evalue": 4.8e-65, "description": "Internal stop codon at 349125. Frameshift due to insertion around 348914.", "psc": { "uniref90_id": "UniRef90_UPI0030CD7D5B", "product": "adhesin", "uniref50_id": "UniRef50_A0A7M3VDG5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0030CD7D5B", "UniRef:UniRef50_A0A7M3VDG5" ] } }, "id": "KMADJIBOEG_657", "locus": "GCA000292445_00642" }, { "type": "cds", "sequence": "contig_1", "start": 349584, "stop": 349823, "strand": "+", "frame": 3, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7M3VDQ4", "UniRef:UniRef50_UPI001ED95422", "UniRef:UniRef90_UPI00031A6C38" ], "nt": "TTGACTTTCACTAATAAGAATAACCCGCAACGAAATCAGTTGTTGCTCAGAAGCTTACTAGGAACGATCCCGGTATTGATCAATAAGAGTGGAACGGGAGATGAGTTTAACCATACGAGTGACCAGAAGTGGGATAAAACGAATGAAAAAGAAGGGAACCTCCCTGGGTTTGGGGAGGTGAATGGTGGTTTTTATCAACTAAATAAAAAACTTATTAGCTTATTTTTATTAGGTTTTTAA", "aa": "MTFTNKNNPQRNQLLLRSLLGTIPVLINKSGTGDEFNHTSDQKWDKTNEKEGNLPGFGEVNGGFYQLNKKLISLFLLGF", "aa_hexdigest": "0750aca061ab931b2ac3b774af8b0c64", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001ED95422", "query_cov": 0.8481012658227848, "subject_cov": 0.9852941176470589, "identity": 0.701, "score": 94.0, "evalue": 8.15e-24, "valid": false, "uniref50_id": "UniRef50_UPI001ED95422" }, "pscc": { "uniref50_id": "UniRef50_UPI001ED95422", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED95422" ] }, "genes": [], "truncated": "both", "pseudogene": { "start": 349305, "stop": 349838, "observations": { "mutation": [ 349374, 349464, 349821 ], "stop": [ 349374, 349464, 349821 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00031A6C38", "query_cov": 1.0, "subject_cov": 0.44886363636363635, "identity": 0.9490000000000001, "score": 157.0, "evalue": 1.48e-47, "gene_start": 1, "gene_end": 79, "reference_start": 93, "reference_end": 171, "reference_sequence": "MITHLNKENTRWVFTPNSSPDIWTGAGYRKANNNNNGISLTSVLPSSNSSQQFNPNSDDNKVTQGGGSPAKKTTTYSFLPNSISPTSDWINALTFTNKNNPQRNQLLLRSLLGTIPVLINKSGTGDEFTHTSEQKWDKTNEKDGNLPGFGEVNGGFYQLNKKLISLFLLGFYLFNS" }, "paralog": false, "identity": 0.8361581920903954, "score": 290.0, "evalue": 2.97e-102, "description": "Internal stop codon at 349374, 349464, 349821. Nonsense mutation around 349374, 349464, 349821.", "psc": { "uniref90_id": "UniRef90_UPI00031A6C38", "product": "adhesin", "uniref50_id": "UniRef50_A0A7M3VDQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00031A6C38", "UniRef:UniRef50_A0A7M3VDQ4" ] } }, "id": "KMADJIBOEG_658", "locus": "GCA000292445_00643" }, { "type": "cds", "sequence": "contig_1", "start": 350113, "stop": 350289, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49391", "UniRef:UniRef90_UPI002FCE0920" ], "nt": "ATGGTGAAAAAGTATAAGTTAGATGTACCAAGTGTTTCAACAACCATGAACCAAGTGTTGCAAAACCTCATCCTAGAACAACCTTTGACTAAATATACCTTAAATAGTAGTTTGGCCAAACAAAAGGGTAAGACACAAGCAGTGGTGCATCTTGGTAATGGGCAAGCAAATAATTGA", "aa": "MVKKYKLDVPSVSTTMNQVLQNLILEQPLTKYTLNSSLAKQKGKTQAVVHLGNGQANN", "aa_hexdigest": "172898c9d15f37ea2fe2d81dd0d90e0b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 349962, "stop": 350307, "observations": { "insertion": [ 350001 ], "stop": [ 350107, 350287 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FCE0920", "query_cov": 1.0, "subject_cov": 0.5043478260869565, "identity": 0.81, "score": 90.1, "evalue": 4.16e-22, "gene_start": 1, "gene_end": 58, "reference_start": 50, "reference_end": 106, "reference_sequence": "MLFKSRDVKVKYESSGSNNISFENSNSQNNKPSYVVEFTNSTNVGIKWTMVKKYQLDVPNVSSDMNQVLQDLILEQPLTKYTLNGSLAKEKGKSQVAVHLGGQANNWQSMRHSMA" }, "paralog": false, "identity": 0.7913043478260869, "score": 160.0, "evalue": 1.7e-52, "description": "Internal stop codon at 350107, 350287. Frameshift due to insertion around 350001.", "psc": { "uniref90_id": "UniRef90_UPI002FCE0920", "uniref50_id": "UniRef50_Q49391", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FCE0920", "UniRef:UniRef50_Q49391" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6384.342400000003, "isoelectric_point": 9.903326988220215 }, "id": "KMADJIBOEG_659", "locus": "GCA000292445_00644" }, { "type": "cds", "sequence": "contig_1", "start": 350296, "stop": 350397, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGCAACCAACACAACCTAAACAACAATCCCTCTCCCAATGCATCAACCGGATTTAAATTAACTAAAGGCAATGCTTATAGAAAACTAAGTGAATCCTGA", "aa": "MRNQHNLNNNPSPNASTGFKLTKGNAYRKLSES", "aa_hexdigest": "ed6ae5a1a4ab870461a8a4a49f15b6d7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3690.0259000000005, "isoelectric_point": 10.446408653259276 }, "id": "KMADJIBOEG_660", "locus": "GCA000292445_00645" }, { "type": "cds", "sequence": "contig_1", "start": 350476, "stop": 351018, "strand": "+", "frame": 1, "gene": null, "product": "Cytadherence-related ORF6-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HQG9" ], "nt": "ATGGCAGCAGAGGATGCGCCCAGTGTTTCAACAAGTGGGGGTGGTTCTTCTGGAAAATTTAAAAAATACCTCAACACCAAACAAGCGTTAGAAAGCATCGGTATCTTGTTTGATGGGGATGGAATGAGGAATGTTATCACCCAGCTCTATTATGCTTCAACTAGTAAGCTAGCAGTCACTAACAACCACATTGTCGTGATGGGTAACAGCTTTCTACCCAGCATGTGGTACTGGGTGGTGGATAGGAGTGCAACAACTGATTCATCATCAAAACCCACCTGGTTTGCTAACACCAATTTAGATTGGGGGGAAGATAAACAAAAACAATTTGTAGAGAATCAGTTGGGGTATAAGGAAACTACCAGTACCAATTCCCACAACTTCCATTCCAAATCTTTCACCCAACCTGCATATCTGATCAGTGGCATTGACAGTGTCAATGATCAGTTGATCTTCAGTGGTTTTAAAGCGGGCAGCGTGGGGTATGATAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTACCAAAGACCAAGCACTTGCTTGA", "aa": "MAAEDAPSVSTSGGGSSGKFKKYLNTKQALESIGILFDGDGMRNVITQLYYASTSKLAVTNNHIVVMGNSFLPSMWYWVVDRSATTDSSSKPTWFANTNLDWGEDKQKQFVENQLGYKETTSTNSHNFHSKSFTQPAYLISGIDSVNDQLIFSGFKAGSVGYDSSSSSSSSSSTKDQALA", "aa_hexdigest": "2847db7a0456fef07dfd73e3cd051eb3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003305ECA5", "query_cov": 0.8611111111111112, "subject_cov": 0.8611111111111112, "identity": 0.51, "score": 149.0, "evalue": 9.09e-43, "valid": false, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_A0AB33HQG9" }, "pscc": { "uniref50_id": "UniRef50_A0AB33HQG9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB33HQG9" ], "product": "Cytadherence-related protein ORF6 homolog" }, "genes": [], "id": "KMADJIBOEG_661", "locus": "GCA000292445_00646" }, { "type": "cds", "sequence": "contig_1", "start": 351064, "stop": 351336, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05220.18" ], "nt": "GTGACCAACGACACGGGTTTAAATGGTCCAATCAATGGGAGTTTTTCAATCCAAGACACCTTCAGCTTTGTTGTTCCTTATTCATCGAATCATACAAATTCAAGTGGTTCATCAGGAACCATTAAAACGGCTTATCCGGTGAAAAACACAGAGAAAGCAAATGTAGCGATCAATTCTTTGATCAACGCTACGCCGTTGAATAGTTATGGGGATGAGGGGGTTGGGGTTAGAAATATTTTTATGAGTTTAAAACAAGGTAATGCAGATAACTAG", "aa": "MTNDTGLNGPINGSFSIQDTFSFVVPYSSNHTNSSGSSGTIKTAYPVKNTEKANVAINSLINATPLNSYGDEGVGVRNIFMSLKQGNADN", "aa_hexdigest": "7f1b9cd2dce46c0b90cf0123633787f0", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05220.18", "name": "MgpC", "length": 76, "aa_cov": 0.8444444444444444, "hmm_cov": 0.9868421052631579, "evalue": 5.567692504786559e-23, "score": 76.6001968383789, "start": 3, "stop": 78 } ], "seq_stats": { "molecular_weight": 9460.238899999998, "isoelectric_point": 5.527614784240721 }, "id": "KMADJIBOEG_662", "locus": "GCA000292445_00647" }, { "type": "cds", "sequence": "contig_1", "start": 351302, "stop": 351565, "strand": "-", "frame": 3, "gene": null, "product": "DUF237 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE37E3E", "UniRef:UniRef90_UPI002FE37E3E" ], "nt": "ATGGATGTTAAATTTATCGTTGATGGTGATTTGGATAGCTATTTAGTTCAAAATACTGATTATCCAGGTTCTTTATTTCAATTTAAAGACAATCAGCTACTTTTCACTTTACACGTAAAAGAGCAGATAAAAGTTCAAGATAAAAATTTTATGGATTTACTTAAACAGCAAAACTTACATAATTTAGATTTAAGAAATGGGGGTACAAAACCTCCAGTTGTTGATTTAGCTAGTTATCTGCATTACCTTGTTTTAAACTCATAA", "aa": "MDVKFIVDGDLDSYLVQNTDYPGSLFQFKDNQLLFTLHVKEQIKVQDKNFMDLLKQQNLHNLDLRNGGTKPPVVDLASYLHYLVLNS", "aa_hexdigest": "66d256db4ebd8fd771117d257a2d0859", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 351302, "stop": 351817, "observations": { "mutation": [ 351577, 351598, 351619, 351715, 351808 ], "stop": [ 351577, 351598, 351619, 351715, 351808 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FE37E3E", "query_cov": 1.0, "subject_cov": 0.5087719298245614, "identity": 1.0, "score": 177.0, "evalue": 1.53e-55, "gene_start": 1, "gene_end": 87, "reference_start": 85, "reference_end": 171, "reference_sequence": "MSDWLRDKLRSHTNISLNLNKVDVENKENNPTISWKEGGIDFRQEYPYKFKFEIDIKYQGGYELNFWSKFIGPWIGIPSSWKGEMDVKFIVDGDLDSYLVQNTDYPGSLFQFKDNQLLFTLHVKEQIKVQDKNFMDLLKQQNLHNLDLRNGGTKPPVVDLASYLHYLVLNS" }, "paralog": false, "identity": 0.9707602339181286, "score": 332.0, "evalue": 8.89e-119, "description": "Internal stop codon at 351577, 351598, 351619, 351715, 351808. Nonsense mutation around 351577, 351598, 351619, 351715, 351808.", "psc": { "uniref90_id": "UniRef90_UPI002FE37E3E", "product": "DUF237 domain-containing protein", "uniref50_id": "UniRef50_UPI002FE37E3E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FE37E3E", "UniRef:UniRef50_UPI002FE37E3E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FE37E3E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE37E3E" ], "product": "DUF237 domain-containing protein" } }, "id": "KMADJIBOEG_663", "locus": "GCA000292445_00648" }, { "type": "cds", "sequence": "contig_1", "start": 352404, "stop": 352547, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "ATGGATTTTGAAGTTGATTATGAAGGTGGTAACAACGCTGTTAACTTGCAATTTGATTTAAAAGCCCAAACTTCTAATTTTTTGAATTTACAAGAGTTGCAAAATAGTTTTAATGGTAACGATATAAATACTCAATTGTTTTGA", "aa": "MDFEVDYEGGNNAVNLQFDLKAQTSNFLNLQELQNSFNGNDINTQLF", "aa_hexdigest": "1ad09cd3515c2a215f57d5bb4d2c8bbe", "start_type": "ATG", "rbs_motif": "TTATA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 49, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 2.562700566386103e-10, "score": 35.19572448730469, "start": 1, "stop": 47 } ], "seq_stats": { "molecular_weight": 5371.724099999999, "isoelectric_point": 4.0500284194946286 }, "id": "KMADJIBOEG_664", "locus": "GCA000292445_00649" }, { "type": "cds", "sequence": "contig_1", "start": 352487, "stop": 353197, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_014894262.1", "SO:0001217", "UniParc:UPI00027B3E39", "UniRef:UniRef100_UPI00027B3E39", "UniRef:UniRef50_UPI00027B3E39", "UniRef:UniRef90_UPI00027B3E39", "PFAM:PF03086.20" ], "nt": "TTGAAAGCTATCAAAAAATCTCGTTTTAAAATTAAAACTTTTCTCTTTGTCTCTTTTGCATCAACTTTAACTGCTGGTGTACTTATTGTCCCTTCTATCTATAATGGCTCAGCTGCAAATTTAAGTCAACCTAGTCTAAGAAAATCAAGTACAACAATAGTAGAGAAAAATAGTATTCTACTCCAACAGCAAACAGCTATAACAGAGCAAGAAGAAAACTATATTGCTAAAGAGAAAGATTATGATAAAAACTTTGACTTATTTCGAGAGAAATTTAAGAATGAAGTTTTAGATCCTAGGAACTTTAATGTTATAGATATTTTTCATCTTTTAAGTGGTTTTAAATCAGGTATTAAAAAAACAGTTGATTTATTAAATAGGCTGCAATCAAGCATTAATGAAACTAACAAAATTTTCCAATATGATGATGAAGATAAATTGCCTAAAGTTCCACAGAATTTTTTTAAGTTCCTTAATGGAAATTTCTTTCCACAATTGCATCCAAAAGGTTTAAACATTACTGAAAGTATTGTTGAAATATTTAATCAATACAACTTAAAGAAAATTCAATTAAAAGACTTTAATCTTGAATTGGTTAAAAAAATGACATTGTTATTAAAGATAAGATTCGTTATAGCTTTGAAATCAAAATGGATTTTGAAGTTGATTATGAAGGTGGTAACAACGCTGTTAACTTGCAATTTGATTTAA", "aa": "MKAIKKSRFKIKTFLFVSFASTLTAGVLIVPSIYNGSAANLSQPSLRKSSTTIVEKNSILLQQQTAITEQEENYIAKEKDYDKNFDLFREKFKNEVLDPRNFNVIDIFHLLSGFKSGIKKTVDLLNRLQSSINETNKIFQYDDEDKLPKVPQNFFKFLNGNFFPQLHPKGLNITESIVEIFNQYNLKKIQLKDFNLELVKKMTLLLKIRFVIALKSKWILKLIMKVVTTLLTCNLI", "aa_hexdigest": "41ec768ffc75ebeb8b215c559bacedf7", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3E39", "ncbi_nrp_id": "WP_014894262.1", "uniref100_id": "UniRef100_UPI00027B3E39", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3E39", "RefSeq:WP_014894262.1", "UniRef:UniRef100_UPI00027B3E39" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3E39", "uniref90_id": "UniRef90_UPI00027B3E39" }, "psc": { "uniref90_id": "UniRef90_UPI00027B3E39", "uniref50_id": "UniRef50_UPI00027B3E39" }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3E39", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3E39" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 159, "aa_cov": 0.6483050847457628, "hmm_cov": 0.9811320754716981, "evalue": 3.337709760784525e-36, "score": 119.92766571044922, "start": 72, "stop": 224 } ], "seq_stats": { "molecular_weight": 27332.783300000043, "isoelectric_point": 9.724556159973144 }, "id": "KMADJIBOEG_665", "locus": "GCA000292445_00650" }, { "type": "cds", "sequence": "contig_1", "start": 353540, "stop": 353992, "strand": "+", "frame": 2, "gene": "cypl", "product": "ABC transporter thiamine pyrophosphate-binding lipoprotein p37/Cypl", "db_xrefs": [ "GO:0005886", "GO:0009897", "GO:0030976", "SO:0001217", "UniRef:UniRef50_P75371", "UniRef:UniRef90_Q49410" ], "nt": "GTGCTTTTCAAAAAATTTACTTGGGTCATTCCAAGTTTGTTTTTAACAATTATTTCAACATCTTTACTTATTAGTTGTGCAACTAAAAGCGATAACACCTTAATATTCAATATTTCACTTGATCATAACGCTGATACATCAATAGAAAAATTCTTTACTGTTTTTTCAAAAAAACTTAGTGAAAAACTGAATAAAAAAATTAATGTTAACTTTAATATAGTTGATGATTCCTTTACAAAAATTAACAATATTCAAGCTAATAAGGCAGATTTTGCTTTTGTTAATTCGCAAGCTATTGCTTCAAATAATTGGTTTGGCTATACGCCATTAATACAAACTTTAACAACAGCTTTTAAAGAAGATTTGGAGCTTGATTATTATGAAGATGGTAATTTACAAAAAAAAGCTGAAAAAACGAATTTACTTTTTCTAAGTCCACCTTACAAAGAATGA", "aa": "MLFKKFTWVIPSLFLTIISTSLLISCATKSDNTLIFNISLDHNADTSIEKFFTVFSKKLSEKLNKKINVNFNIVDDSFTKINNIQANKADFAFVNSQAIASNNWFGYTPLIQTLTTAFKEDLELDYYEDGNLQKKAEKTNLLFLSPPYKE", "aa_hexdigest": "7be5d6fde783d297015932a993fbe979", "start_type": "GTG", "rbs_motif": null, "genes": [ "cypl" ], "truncated": "3-prime", "pseudogene": { "start": 353540, "stop": 354295, "observations": { "mutation": [ 353990, 354011, 354125, 354140 ], "stop": [ 353990, 354011, 354125, 354140 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49410", "query_cov": 1.0, "subject_cov": 0.4076086956521739, "identity": 0.94, "score": 271.0, "evalue": 1.76e-88, "gene_start": 1, "gene_end": 150, "reference_start": 1, "reference_end": 150, "reference_sequence": "MLFKKFTWVIPSLFLTIISTSLLISCATKSDNTLIFNISLDHNADTSIEKFFTVFSKKLSGKLNKKINVNFNIVDDSFTKINNIQANKADFAFVNSQAIASNNWFGYTPLIQTLTTAFKEDLELDYYEDGNLQKKAEKTNLLFLSPPYKEWDDIKQKWTGNRYDFLYEPSKLVSFYRSMILITGSASEITAIKKAWNEKNWNQFMKFGIGHGQTNSASRFELPDLLFRKHFAKNYPGLQNAINSDPDKFAVVRGREIGINKNIKIVFDDANSFSWTQNIKGSKRPFYTPIDPNDRLEILTYSDPLLYDIGIVSNNLSRIYQKAIGEIFIELAQSSEDLYGPSIGYNGYKMINDFEKEVVEIIEKTYGK" }, "paralog": false, "identity": 0.9442231075697212, "score": 466.0, "evalue": 8.68e-168, "description": "Internal stop codon at 353990, 354011, 354125, 354140. Nonsense mutation around 353990, 354011, 354125, 354140.", "psc": { "uniref90_id": "UniRef90_Q49410", "gene": "cypl", "product": "ABC transporter thiamine pyrophosphate-binding lipoprotein p37/Cypl", "uniref50_id": "UniRef50_P75371", "go_ids": [ "GO:0005886", "GO:0009897", "GO:0030976" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49410", "UniRef:UniRef50_P75371", "GO:0005886", "GO:0009897", "GO:0030976" ] }, "pscc": { "uniref50_id": "UniRef50_P75371", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75371" ], "product": "High affinity transport system protein p37" } }, "id": "KMADJIBOEG_666", "locus": "GCA000292445_00651" }, { "type": "cds", "sequence": "contig_1", "start": 354152, "stop": 354364, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTTGGAATTGGTCATGGACAAACAAATTCAGCTTCACGTTTTGAGCTACCTGATCTTTTATTTAGAAAACATTTTGCTAAAAATTATCCCGGATTACAAAATGCAATTAATTCTGATCCCGATAAATTTGCCGTAGTTAGAGGAAGAGAGATAGGTATAAATAAAAACATCAAGATTGTTTTTGATGATGCTAATTCATTTTCTTGA", "aa": "MKFGIGHGQTNSASRFELPDLLFRKHFAKNYPGLQNAINSDPDKFAVVRGREIGINKNIKIVFDDANSFS", "aa_hexdigest": "cfa480c30db29582fa09225b1ac9e7b0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7853.800199999999, "isoelectric_point": 9.598198127746581 }, "id": "KMADJIBOEG_667", "locus": "GCA000292445_00652" }, { "type": "cds", "sequence": "contig_1", "start": 354455, "stop": 354646, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATGACATTGGTATTGTTAGCAACAATTTATCAAGGATATATCAAAAAGCTATTGGTGAAATTTTTATTGAGTTAGCGCAATCAAATGAAGATCTATATGGGCCTTCAATTGGTTATAACGGCTATAAAATGATTAATGATTTTCAGAAAGAAGTTGTTGAAATAATTGAAAAAACCTATGGAAAATAA", "aa": "MYDIGIVSNNLSRIYQKAIGEIFIELAQSNEDLYGPSIGYNGYKMINDFQKEVVEIIEKTYGK", "aa_hexdigest": "12dc3e532ce2180790f2dbe48dd831ff", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7225.121799999999, "isoelectric_point": 4.648372459411622 }, "id": "KMADJIBOEG_668", "locus": "GCA000292445_00653" }, { "type": "cds", "sequence": "contig_1", "start": 354636, "stop": 354968, "strand": "+", "frame": 3, "gene": "p29", "product": "putative ABC transporter ATP-binding protein p29", "db_xrefs": [ "COG:COG1120", "COG:PH", "GO:0005524", "GO:0016887", "GO:0042626", "GO:0043190", "SO:0001217", "UniRef:UniRef50_P47532", "UniRef:UniRef90_P47532" ], "nt": "ATGGAAAATAAACCAATTCTTTCTTTTGAAAAAGTATCAATAATATACAAAAAAGCTCCACTTTTGCAAAACATTAGTTTTAAAGTAATGGCAAAGGAAAATGTTTGCTTATTAGGTAAGTCTGGAGTTGGAAAATCAAGCCTTTTAAACAGTGTTACTAATACGAAAATAGTTAAAAGTGGGTTAGTTTACTTTGATGGTGTTGCTTCAAACAAAAAGGAATACAAAAAACTGAAAAAACAGTGCAGTTATCTGGATCAAATACCAAATTTAATTGACACTGATTATGTATATGAAGCAATTTTAAGATCTGCTAAACAAAAATTAACTTGA", "aa": "MENKPILSFEKVSIIYKKAPLLQNISFKVMAKENVCLLGKSGVGKSSLLNSVTNTKIVKSGLVYFDGVASNKKEYKKLKKQCSYLDQIPNLIDTDYVYEAILRSAKQKLT", "aa_hexdigest": "1d7b689a5bce4d2c65bbf50590f5ae49", "start_type": "ATG", "rbs_motif": null, "genes": [ "p29" ], "truncated": "3-prime", "pseudogene": { "start": 354636, "stop": 355271, "observations": { "mutation": [ 354966, 354999 ], "stop": [ 354966, 354999 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47532", "query_cov": 1.0, "subject_cov": 0.4489795918367347, "identity": 1.0, "score": 214.0, "evalue": 1.04e-68, "gene_start": 1, "gene_end": 110, "reference_start": 1, "reference_end": 110, "reference_sequence": "MENKPILSFEKVSIIYKKAPLLQNISFKVMAKENVCLLGKSGVGKSSLLNSVTNTKIVKSGLVYFDGVASNKKEYKKLKKQCSYLDQIPNLIDTDYVYEAILRSAKQKLTWLQKLICFEPKWIKDKILAILKEVNLNDYVSCIIKDLSAGQKQRVEIAKLFFKSPKLLLVDEPTTGLDPLTASKIMDLITDFVKREKITLVFVTHDIDLALKYSTRIIALKNHALVLDRLTEKLTKEQLYKIYDN" }, "paralog": false, "identity": 0.985781990521327, "score": 397.0, "evalue": 2.78e-143, "description": "Internal stop codon at 354966, 354999. Nonsense mutation around 354966, 354999.", "psc": { "uniref90_id": "UniRef90_P47532", "gene": "p29", "product": "Probable ABC transporter ATP-binding protein p29", "uniref50_id": "UniRef50_P47532", "cog_id": "COG1120", "cog_category": "PH", "go_ids": [ "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47532", "UniRef:UniRef50_P47532", "COG:COG1120", "COG:PH", "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_P47532", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47532" ], "product": "Probable ABC transporter ATP-binding protein p29" } }, "id": "KMADJIBOEG_669", "locus": "GCA000292445_00654" }, { "type": "cds", "sequence": "contig_1", "start": 355191, "stop": 355373, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTAATTACTGATTTTGTGAAAAGAGAAAAAATAACTTTGGTTTTTGTAACACATGATATAGATTTAGCACTGAAATATAGCACCAGAATTATAGCACTTAAAAATCATGCTTTAGTGTTAGATAAATTAACAGAAAAACTAACAAAAGAACAACTTTATAAAATTTATGATAACTAA", "aa": "MDLITDFVKREKITLVFVTHDIDLALKYSTRIIALKNHALVLDKLTEKLTKEQLYKIYDN", "aa_hexdigest": "807d33cdfa63f82eaa9d723bf5f3a68f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7093.287699999999, "isoelectric_point": 8.004668617248537 }, "id": "KMADJIBOEG_670", "locus": "GCA000292445_00655" }, { "type": "cds", "sequence": "contig_1", "start": 355680, "stop": 355919, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTCAAAAAGCTTTAAAACCACTTCCTTCTCTAATTTTTTGGTTAAAAAAATACTTTAAATTGCTGTTTTCAAAAAATGTAGTAATGTATTTTGTATTCCATAAAAAGCTAAACCAGCTAATCGTTAGTGTAGCTGCTAGTTGTTTATTAAATAAATTACTAAATAAAAAACCAAATAAAAGTACGGGAAATGCTCTTAAAACTATAGTTATTAACCTGATCGGTAGAGCAATGTAA", "aa": "MVQKALKPLPSLIFWLKKYFKLLFSKNVVMYFVFHKKLNQLIVSVAASCLLNKLLNKKPNKSTGNALKTIVINLIGRAM", "aa_hexdigest": "dc56122d310ee3d8bbad3e7935a9c231", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9020.056399999994, "isoelectric_point": 10.59965000152588 }, "id": "KMADJIBOEG_671", "locus": "GCA000292445_00656" }, { "type": "cds", "sequence": "contig_1", "start": 356059, "stop": 356217, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAGTTTGATCTTGTATTAATTCCATTAGTTGTTTTAATAACCTTTCTAATTTTTATAGAAGTTGTTGTTTTTTTATTATCAAGTTTTATTTTTGAGAAAAATAGTGAAGATTTAAGACCAATTTTAAAAACAACAGTTATTGAAAAACGAAAGTGA", "aa": "MQFDLVLIPLVVLITFLIFIEVVVFLLSSFIFEKNSEDLRPILKTTVIEKRK", "aa_hexdigest": "e28d10b62760ea01af6a11f6add2d420", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6109.389899999998, "isoelectric_point": 6.09100284576416 }, "id": "KMADJIBOEG_672", "locus": "GCA000292445_00657" }, { "type": "cds", "sequence": "contig_1", "start": 356236, "stop": 356730, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTATTGTAGTACTAATTAGTCTTAGTTTAGCCAATTTAGTGACTATTGATTACAGAATTAACGATGCTGAATTTTTACAAGATTTTTTTAACCACTTTTTTCAGTTAAAAAGTAATCTTTTTTCAAGTAATGATCCTAATATAAACCCAATTTTAATGTTAGTTAAACTTACAACTCAAGCTATTAGTTTAATTAGCTTGGTTGTAATCTTTTCTATTCTCTTTGGTTTTATATCATGTAATTTATTTAAAAAGAGATTTTCAATTAGTTTTAAGATCTTATTACTTTTTGTTAGAGTAGTTCCTAGTATTCTGTTGTTTAGGTTACTAGATCCTTTATTTCTAGAAGCAAAAACAACTATTATTTTAGTCTTACTAATTAATCATGGTTCAAGTTATGGCCAATTGATGGCAATTAACTTTAATAAGGCAAATCAAAATATCATCAATAACTATAAAAATCATGGTATGACAAAAGATTTTATTTTATGA", "aa": "MFIVVLISLSLANLVTIDYRINDAEFLQDFFNHFFQLKSNLFSSNDPNINPILMLVKLTTQAISLISLVVIFSILFGFISCNLFKKRFSISFKILLLFVRVVPSILLFRLLDPLFLEAKTTIILVLLINHGSSYGQLMAINFNKANQNIINNYKNHGMTKDFIL", "aa_hexdigest": "6172b086d5ffbd5f6d6750761689014a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18794.212999999992, "isoelectric_point": 9.561451148986816 }, "id": "KMADJIBOEG_673", "locus": "GCA000292445_00658" }, { "type": "cds", "sequence": "contig_1", "start": 356737, "stop": 356994, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGTTGAAAATAAACCTAATTTAATAAACATTACCAGTGATGCTTATGATAGTGGAATTAGGGATTTAATTTTGTTTGGTAGTTTTGGCGGTTCAATTATTGGTAGTAGAATTACTAATTTTTTTGAAAGAGCTCAATTTGATAATCTAGGTTCTGTTACAATCCCATTAATGGTTTATCTAATTGCAATAGAAATAATTTTTCTTTCAGTTAGATTAACTAGAATTTCAGTTTTTAAGAACTACCTTTACTAA", "aa": "MLVENKPNLINITSDAYDSGIRDLILFGSFGGSIIGSRITNFFERAQFDNLGSVTIPLMVYLIAIEIIFLSVRLTRISVFKNYLY", "aa_hexdigest": "14c24271b3360f385bb04a95b41160dd", "start_type": "TTG", "rbs_motif": "ATTTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9610.111899999993, "isoelectric_point": 6.081283378601074 }, "id": "KMADJIBOEG_674", "locus": "GCA000292445_00659" }, { "type": "cds", "sequence": "contig_1", "start": 356991, "stop": 357407, "strand": "-", "frame": 2, "gene": "ruvX", "product": "Holliday junction resolvase RuvX", "db_xrefs": [ "COG:COG0816", "COG:JLK", "EC:3.1.-.-", "EC:3.1.21.10", "GO:0000967", "GO:0004518", "GO:0005737", "GO:0006364", "RefSeq:WP_014894266.1", "SO:0001217", "UniParc:UPI00027B3AD9", "UniRef:UniRef100_UPI00027B3AD9", "UniRef:UniRef50_Q9ZB76", "UniRef:UniRef90_Q9ZB76" ], "nt": "GTGAAATATATATTAGCAATTGATTTTGGCTTGAAAAAAATAGGTACAGCAATTGCAAATACACTAGATAAATATCCTTCTGCATTTCATGTTTTTGAAGTAAAAAATAATTTCAAAACTGCTGTTAACAACTTATTTTTGCGAATTAAAAACGATGGGTATGAACTTGAAAAAATAGTGATAGGTTTTCCCAAATTTCACTATTATTCTGATATTCAAAAGGCAATTAAATCATTTAAACAACTTTTAGAACAACGTTTTAATTTGCCAATAATTTTGGTTGATGAAAGTAATACTACAAGTGCTGTTAAAGATAAGCTTATAACAATGGATTTAAAACATAAGGATTTTAAAAAGGCTAAAGATACATTAGCTGCAGTTTTAATATTAGAGCGCTTCTTTCAAAACTATCATTAG", "aa": "MKYILAIDFGLKKIGTAIANTLDKYPSAFHVFEVKNNFKTAVNNLFLRIKNDGYELEKIVIGFPKFHYYSDIQKAIKSFKQLLEQRFNLPIILVDESNTTSAVKDKLITMDLKHKDFKKAKDTLAAVLILERFFQNYH", "aa_hexdigest": "b6f0b927a65d008eb135df63a9afff66", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3AD9", "ncbi_nrp_id": "WP_014894266.1", "uniref100_id": "UniRef100_UPI00027B3AD9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3AD9", "RefSeq:WP_014894266.1", "UniRef:UniRef100_UPI00027B3AD9" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3AD9", "uniref90_id": "UniRef90_Q9ZB76" }, "psc": { "uniref90_id": "UniRef90_Q9ZB76", "gene": "ruvX", "product": "Holliday junction resolvase RuvX", "ec_ids": [ "3.1.-.-", "3.1.21.10" ], "uniref50_id": "UniRef50_Q9ZB76", "cog_id": "COG0816", "cog_category": "JLK", "go_ids": [ "GO:0000967", "GO:0004518", "GO:0005737", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZB76", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZB76" ], "product": "Putative pre-16S rRNA nuclease" }, "genes": [ "ruvX" ], "id": "KMADJIBOEG_675", "locus": "GCA000292445_00660" }, { "type": "cds", "sequence": "contig_1", "start": 357411, "stop": 357911, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGATAGTTCAATTTTTAAAGTTGAGTTAAAAGAATTGCAACAAAGGCTAGATAAATTTATCTTACCTGAAAAAATTACCCAATTAAGAGATGCATCTGATACTTTATTAGCTTTAAAAAATGATATTAACCAGTTAAAAACAAAAAACTATAAAGTATCACAGCAAGCTTTAGCCTTATCAATTAAAAAGCAATTATTATCCTTAGTAGATAAAAATAAAAGTTATGTAATTGCTACTTTTAATGACGTAGAACCTAAACTATTGCTACAAACAATACATGATGTTTTCAATCAAAATCAAACTAAAAATTTCTTGATAATTAATCAATTCAATGGAAGTAATTCATTTATTGTTATAGGAAATAAAATTACCACCATTATTGAAAAATTAAGAAATAGTTTTAATTTAAAGGGCGGAGGCAATGATAAGTTATTTAGAGGTTCTTTTCAGGATAATGTTACCCCTCAAAAGCTTAATGAATTGTTTCAAAAATAA", "aa": "MIDSSIFKVELKELQQRLDKFILPEKITQLRDASDTLLALKNDINQLKTKNYKVSQQALALSIKKQLLSLVDKNKSYVIATFNDVEPKLLLQTIHDVFNQNQTKNFLIINQFNGSNSFIVIGNKITTIIEKLRNSFNLKGGGNDKLFRGSFQDNVTPQKLNELFQK", "aa_hexdigest": "4e9327ac6385fd105e403c193f2eac0d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19028.773699999998, "isoelectric_point": 9.667566108703614 }, "id": "KMADJIBOEG_676", "locus": "GCA000292445_00661" }, { "type": "cds", "sequence": "contig_1", "start": 358014, "stop": 358301, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07973.19" ], "nt": "TTGAATCGTCATTTAACAAGAAATGAACTTGAGAAAGTAGAAAATAAAATTCGCTCTTTGATTAAACAAAAAATAAGCTCAGAAGAGATTTTTACTGATTTTGAAGGGAGTCAAAAACTAAATGCAATTGCTTATTTTGAAGAGGAATATTCTCAACATGAAATATTAAGAGTGATCCGCTTTGGTGATTATAGTGTTGAGTTGTGTGGTGGCACTCATGTAGCTAACACAGCTTCAATTGAAGATTGTTTTATTACTGATTTCTATTCTTTAGGAGCTGGAAGATGA", "aa": "MNRHLTRNELEKVENKIRSLIKQKISSEEIFTDFEGSQKLNAIAYFEEEYSQHEILRVIRFGDYSVELCGGTHVANTASIEDCFITDFYSLGAGR", "aa_hexdigest": "c3fd4e49eeefcf96237ada3ef9f3cf15", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07973.19", "name": "tRNA_SAD", "length": 31, "aa_cov": 0.3263157894736842, "hmm_cov": 1.0, "evalue": 1.3923427480698141e-12, "score": 42.600894927978516, "start": 56, "stop": 86 } ], "seq_stats": { "molecular_weight": 10952.125299999989, "isoelectric_point": 5.13400478363037 }, "id": "KMADJIBOEG_677", "locus": "GCA000292445_00662" }, { "type": "cds", "sequence": "contig_1", "start": 358431, "stop": 358829, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCAAACATCGTTCTATCTCTAAGCAAAATAACCAAACTATAAATTTTGAAAAACAAAATATTAATTTAGTTAATTTCAAAACTAAAAGTACTTTTTTTTATCACAAAAATAAAATTAATGCTAAGGTAATTGGTCTTTTTGATGAAAATTATTTACCAGTTAAAGAACTTAATAATCAAAGTGGTTATGTAGTTTTTGACCAAACAGTTTTATATGCTACTTCTGGAGGACAGAGATATGATGAAGGAAGTTGCATTAATCATTCTAATAATAATGATCAAAAAATCAGTTTTCAAGGTGTATTTAAAGGACCTAATAAACAACACTTCCACTACTTTTTAGTAGGTAGTTTTAAACTCAATGATCAAGTAACTTTATCACATGATGAAACTTGA", "aa": "MAKHRSISKQNNQTINFEKQNINLVNFKTKSTFFYHKNKINAKVIGLFDENYLPVKELNNQSGYVVFDQTVLYATSGGQRYDEGSCINHSNNNDQKISFQGVFKGPNKQHFHYFLVGSFKLNDQVTLSHDET", "aa_hexdigest": "ef1292213d18431e6bc67b5fc9f8f670", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15240.800699999993, "isoelectric_point": 9.163229942321777 }, "id": "KMADJIBOEG_678", "locus": "GCA000292445_00663" }, { "type": "cds", "sequence": "contig_1", "start": 358848, "stop": 359282, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAGAACAGAGTTATTTTCGGATTATTGCTGATCACTTTAAAGCAATCACTTTTACCATTTCAGAAGGAGTTTTACCTGGTCCTAATGAGAGAAATTATGTAGTAAGAAGACTTTTAAGACGTGCTTTAATAGCTTGTAAGAAATTGCAATTAAACTTAGCATTTATTGAAAAGATAATAGATGAAATCATCGCTTCATATGAGAATTATTATCAACATTTAAAAGCTAAAAATGAAACTGTTAAACAGGTAGTTTTAAAAGAGATTAATGCATTTAATAAAACGATTGATTTAGGTTTAGTGCTGTTTGAAAAAAGTGTTAAAAACAATACTCTAACTCCCCAATTAGCATTTCAATTGAACGAAACATACGGTTTTCCTGTTGAAATAATAAGAGAACTAGTTAATCAAAAAGGTTTAACTATTGATTGA", "aa": "MKEQSYFRIIADHFKAITFTISEGVLPGPNERNYVVRRLLRRALIACKKLQLNLAFIEKIIDEIIASYENYYQHLKAKNETVKQVVLKEINAFNKTIDLGLVLFEKSVKNNTLTPQLAFQLNETYGFPVEIIRELVNQKGLTID", "aa_hexdigest": "5c4cb1efa44df57df1e6ea4729139165", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16687.264799999986, "isoelectric_point": 9.161424827575683 }, "id": "KMADJIBOEG_679", "locus": "GCA000292445_00664" }, { "type": "cds", "sequence": "contig_1", "start": 359700, "stop": 359882, "strand": "-", "frame": 2, "gene": null, "product": "alanine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920MQJ2" ], "nt": "GTGGGTTTTACTTCAAGACATCAAACTTTGTTTGAGATGCTTGGAAATTTTTCAATCGGTGATTATTTTAAAACAGAAGCAATTGATTTTGCTTTTGATCTTTTAGTTAATTATTATCAGCTAGATCCTAAGCGTTTTTATATCACTGTTTATGAAGATGATGAAACTACTTATAAAAGATGA", "aa": "MGFTSRHQTLFEMLGNFSIGDYFKTEAIDFAFDLLVNYYQLDPKRFYITVYEDDETTYKR", "aa_hexdigest": "7d6e2918c56b5049e14b9f42f329ec5e", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A920MQJ2", "query_cov": 0.9166666666666666, "subject_cov": 0.9649122807017544, "identity": 0.564, "score": 65.5, "evalue": 5.5e-13, "valid": false, "product": "alanine--tRNA ligase", "uniref50_id": "UniRef50_A0A920MQJ2" }, "pscc": { "uniref50_id": "UniRef50_A0A920MQJ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920MQJ2" ], "product": "alanine--tRNA ligase" }, "genes": [], "id": "KMADJIBOEG_680", "locus": "GCA000292445_00665" }, { "type": "cds", "sequence": "contig_1", "start": 360327, "stop": 360902, "strand": "-", "frame": 2, "gene": null, "product": "putative MG293-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75367" ], "nt": "ATGCATAACAAGCAATTGCTTTTAGCACATAGGGGTTATTCATTCATTGCTCCAGAAAACACCAAACTAGCATTTGATTTAGCTTTTGAATATTGTTTTGATGGAATAGAGCTTGATGTTCATTTAACTAAAGATGAACAGTTAGTTATCATTCATGATGAGACAACATTGAGAACCGCATTAGTTAATAAGGAGGTTGAGTTTGAATCATTAGTTAGTTTAAAAAGAGATGATCATAGTGCTTTTTTTCACCTTAAAATTCAATTTCAATCGATCCTAACTTTAAAAGAGTTCTTAGATCTTTATTTAGATAAATTTAAGTTAATCAATATTGAGATTAAAACTGATCAAAAACCATATTTAGGAATTGAAAAGAAGCTTGTTGACCTAGTTAAAGGTTATGGTAAAAAAGCAATAGATAAGATCTTGTTTTCATCCTTTAACTTTGAATCTTTGCAAAAAGTTTATGATTTAGATAATAGTTACAAAAAAGGTTTTTTATTTTGGACTAAAAAACAGTTTGAAACAATTAGTACAGCTAGAATCCAAAAGATTTGTCAATTCCTCCACCCATGA", "aa": "MHNKQLLLAHRGYSFIAPENTKLAFDLAFEYCFDGIELDVHLTKDEQLVIIHDETTLRTALVNKEVEFESLVSLKRDDHSAFFHLKIQFQSILTLKEFLDLYLDKFKLINIEIKTDQKPYLGIEKKLVDLVKGYGKKAIDKILFSSFNFESLQKVYDLDNSYKKGFLFWTKKQFETISTARIQKICQFLHP", "aa_hexdigest": "052932c87959439b70799977b107973e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021153946", "query_cov": 1.0104712041884816, "subject_cov": 0.8041666666666667, "identity": 0.56, "score": 192.0, "evalue": 1.91e-58, "valid": false, "product": "glycerophosphodiester phosphodiesterase family protein", "uniref50_id": "UniRef50_P75367" }, "pscc": { "uniref50_id": "UniRef50_P75367", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75367" ], "product": "Uncharacterized protein MG293 homolog" }, "genes": [], "id": "KMADJIBOEG_681", "locus": "GCA000292445_00666" }, { "type": "cds", "sequence": "contig_1", "start": 360916, "stop": 361098, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACTGCTGAAGTTGGTAAGAAATATACAGTTGCAGGACAAAGTAATACTAGTAACACTGGTTATCAAATTATCGCTGCTATTTGTTTATCCATTGCTTTGTTTGGTTTGATATGTGGATCAATTGTTTTTATCTCAAATAGTATGGAAATTAAACGATTAAATAAAGCTTCATACCGTTGA", "aa": "MTAEVGKKYTVAGQSNTSNTGYQIIAAICLSIALFGLICGSIVFISNSMEIKRLNKASYR", "aa_hexdigest": "cf0d1d4f4608e3693ae2dd3f8843f71b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6447.482499999996, "isoelectric_point": 9.445279121398926 }, "id": "KMADJIBOEG_682", "locus": "GCA000292445_00667" }, { "type": "cds", "sequence": "contig_1", "start": 361108, "stop": 361539, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGATGTATGTGTTTCAAAGCACTTTTGCTTATTACCTTTTACAGATGTTGCAAAATGCATTTTTAGCACCTGTAGTATTAGTAACTGTAATTGGGGGAATTAGAACTTATGCACTGAGAAGTGCTGTTAGTGTTTATCTTGGTAGATTAGCAGATAAGTGTAAAAGTTATATCTTGTTTTTAATGATATGCACTGTTCTTGGTATTGTATTTGTTCTAGCATTTATTCTACTTGGTTTTGTACAAACAAATTCTGCTAACATCACTTTAATTACTTTCTCTTCCATTCTCTATATCTTTACTGGTATCTTATCATGGGGAATGGTTACAGTCAGATATAACCAAATAGGTGAAATTGATATTGGTAAAAATAACTATGCATCTAGTGTTGGTTTACTTAGTTTTATTGGTTTTTCTACTGATGGATGA", "aa": "MGMYVFQSTFAYYLLQMLQNAFLAPVVLVTVIGGIRTYALRSAVSVYLGRLADKCKSYILFLMICTVLGIVFVLAFILLGFVQTNSANITLITFSSILYIFTGILSWGMVTVRYNQIGEIDIGKNNYASSVGLLSFIGFSTDG", "aa_hexdigest": "363dafeb8c1311021ac0188a0209532b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15732.50129999998, "isoelectric_point": 8.975111198425292 }, "id": "KMADJIBOEG_683", "locus": "GCA000292445_00668" }, { "type": "cds", "sequence": "contig_1", "start": 361567, "stop": 361785, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAATTACCAGCATTTTCTATCCATCAGGAGGTAGTGAGAATGCTGATTCAAAACCATTTGCTGCATACGCTTTTATCATTGCCATAATGCTAGTAATTACTGGATTTACAGTTTTGTTTTTTGTTAAAGAAAAGCCAATTAAAAAACAAAGTCAAACCACTTTAGTAAGTTTTAAACGCAATTTAAACCAGATTTTAGTGACCTTAAAAAACTGA", "aa": "MIITSIFYPSGGSENADSKPFAAYAFIIAIMLVITGFTVLFFVKEKPIKKQSQTTLVSFKRNLNQILVTLKN", "aa_hexdigest": "5ef9be8d3febea16d6d72ce3598de9a0", "start_type": "TTG", "rbs_motif": "ATTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8044.470699999997, "isoelectric_point": 9.901779747009275 }, "id": "KMADJIBOEG_684", "locus": "GCA000292445_00669" }, { "type": "cds", "sequence": "contig_1", "start": 362011, "stop": 362319, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATAGTGACAACAAAAAAAGGATTTAAAAAACGCTTAAAGGATTTTTCAAAACAACAAATTCTTGCCTTAATTATTTTGGGAGCAATTGATGTATTTGTAATTGCTGCACCCTATTATGTTAAGAATGTTGTTCCTAACCTCCACTTGTACTTAGGAATTACAGAGGATGAAGTTGCTACTGTTACTTCTATCATTGGTTATGTAACGCTTGCCACTCAACTACCAGGAGGGTTTTTAGCCAATCGCTTCAGTTCTAGAAAATTGTTATTTCTATCAGCAATTACTACTGGAGCAATTACTTCTTGA", "aa": "MIVTTKKGFKKRLKDFSKQQILALIILGAIDVFVIAAPYYVKNVVPNLHLYLGITEDEVATVTSIIGYVTLATQLPGGFLANRFSSRKLLFLSAITTGAITS", "aa_hexdigest": "486064f53d1b30e3074589ebb62e8669", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11118.077299999986, "isoelectric_point": 9.850978660583493 }, "id": "KMADJIBOEG_685", "locus": "GCA000292445_00670" }, { "type": "cds", "sequence": "contig_1", "start": 362437, "stop": 362673, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF20258.4" ], "nt": "TTGAAAACAACAATTTTATTGGATCAATTTAACTGGTTGTATACACCAAAGCAACTTCCTAGTCAAGTTCTGGTAAGAATTAGACATGCTCAAAAACCCGAAATTGCAAAGTTGAAATTATTATCAGATAATAAACTGGAAATAACATTTAAAAATCCTGTTATAAGTGTTGCATCTGGACAGTTTGGTGTATTATATACACTTGATCAAATTTGTTTAGGAGCAGGATTAATTTAA", "aa": "MKTTILLDQFNWLYTPKQLPSQVLVRIRHAQKPEIAKLKLLSDNKLEITFKNPVISVASGQFGVLYTLDQICLGAGLI", "aa_hexdigest": "c1d2718396eccacb5089be333c7ea795", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF20258.4", "name": "tRNA_Me_trans_C", "length": 77, "aa_cov": 0.9743589743589743, "hmm_cov": 0.987012987012987, "evalue": 4.66580532958765e-20, "score": 66.9324722290039, "start": 3, "stop": 78 } ], "seq_stats": { "molecular_weight": 8781.335199999998, "isoelectric_point": 9.517612648010253 }, "id": "KMADJIBOEG_686", "locus": "GCA000292445_00671" }, { "type": "cds", "sequence": "contig_1", "start": 362944, "stop": 363249, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03054.22" ], "nt": "ATGATTCAAAGTTTCAAAAAAGGGTTAACCCCAAATCCAGACATCTGGTGTAATCGTTTTATTAAGTTTGGTTTATTGCATGATTTTTGTAAGCAAATTAACCCTAATTCTCTTTTTGCAACTGGTCATTATGCCAAAATAAACATGATAGAAAATCAGCCTTTGCTTTCTATTCCTAAAGATACCAATAAAGATCAAACTTATTTTTTAGCAAATGTTAAAAAAGAACAATTTCAGAATGTTATTTTTCCTTTAGCAGATTTAAAAAAAATAACGGTGAGAAATATTGCTAGAGAAAATAATTGA", "aa": "MIQSFKKGLTPNPDIWCNRFIKFGLLHDFCKQINPNSLFATGHYAKINMIENQPLLSIPKDTNKDQTYFLANVKKEQFQNVIFPLADLKKITVRNIARENN", "aa_hexdigest": "5c07ac57deafb152956cd58897f2ad07", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03054.22", "name": "tRNA_Me_trans", "length": 102, "aa_cov": 0.9900990099009901, "hmm_cov": 0.9901960784313726, "evalue": 1.4037362194738668e-35, "score": 117.92342376708984, "start": 1, "stop": 100 } ], "seq_stats": { "molecular_weight": 11753.529599999983, "isoelectric_point": 9.554230690002441 }, "id": "KMADJIBOEG_687", "locus": "GCA000292445_00672" }, { "type": "cds", "sequence": "contig_1", "start": 363282, "stop": 363539, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02540.23", "PFAM:PF03054.22" ], "nt": "ATGTCAATTATTGCAAAAACAGTTTTTATAGGTTTAAGTGGTGGTGTTGATTCTGCTGTTAGTGCTTTACTTTTAAAAAAGCAATACCAAGAAGTTATTGGTGTTTTTATGGAATGTTGGGATGAGACACTTAATAATGATTTTTATGGTCATAAGAAAATAAATAATAACAAATCAGGTTGTTCATCTTTTCAAGACCTCCAACAGGCTAAAAAAATCGCTAATTCTTTAGGAATTAAGTTAATAAAAAAAACTTAA", "aa": "MSIIAKTVFIGLSGGVDSAVSALLLKKQYQEVIGVFMECWDETLNNDFYGHKKINNNKSGCSSFQDLQQAKKIANSLGIKLIKKT", "aa_hexdigest": "4690341f6cf0670cbd574b35a1dd573a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02540.23", "name": "NAD_synthase", "length": 40, "aa_cov": 0.4470588235294118, "hmm_cov": 0.975, "evalue": 2.796840866354784e-08, "score": 28.299665451049805, "start": 5, "stop": 42 }, { "id": "PF03054.22", "name": "tRNA_Me_trans", "length": 77, "aa_cov": 0.9058823529411765, "hmm_cov": 0.8831168831168831, "evalue": 5.136552596312872e-20, "score": 67.1073226928711, "start": 6, "stop": 82 } ], "seq_stats": { "molecular_weight": 9368.78769999999, "isoelectric_point": 9.200492668151856 }, "id": "KMADJIBOEG_688", "locus": "GCA000292445_00673" }, { "type": "cds", "sequence": "contig_1", "start": 363542, "stop": 363931, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MG296", "db_xrefs": [ "RefSeq:WP_014894269.1", "SO:0001217", "UniParc:UPI00027B3BFF", "UniRef:UniRef100_UPI00027B3BFF", "UniRef:UniRef50_P75364", "UniRef:UniRef90_P47538" ], "nt": "ATGAAACCACAACTCATAGCTTTTAAAAAATTTTTACAAACTGAATTTCAAGCGGTTGATTTTGAGACTTTTCGCATTAACTTTAATTTGTGTTTAAAACGAGAACAAGACAATATTGTTATTTATGAAGATGATGATTATGATGACCAACCATTTTTTTTCAAACCAATGTTAAGTGATGGTTTTTTTATCCAAACTGAAGTAATTAAACAGCTAGATTATCTAGCAAAAGTTGTAGAAAATCCTAAAGATAGTGATCAGCAGTGCTGTCAAAATTTTTATGAAGCATTAATAGTGTTTATTTCTGCACTTGCTATTACAAAAGGCATTAATCCTAATAGATTTCACCAAAGATTAGTAAACAAGTTTGCTATTCACGCTGTTTATTAA", "aa": "MKPQLIAFKKFLQTEFQAVDFETFRINFNLCLKREQDNIVIYEDDDYDDQPFFFKPMLSDGFFIQTEVIKQLDYLAKVVENPKDSDQQCCQNFYEALIVFISALAITKGINPNRFHQRLVNKFAIHAVY", "aa_hexdigest": "3d5a4518cd1db8081b9d3c41233c775b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3BFF", "ncbi_nrp_id": "WP_014894269.1", "uniref100_id": "UniRef100_UPI00027B3BFF", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3BFF", "RefSeq:WP_014894269.1", "UniRef:UniRef100_UPI00027B3BFF" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3BFF", "uniref90_id": "UniRef90_P47538" }, "psc": { "uniref90_id": "UniRef90_P47538", "product": "Uncharacterized protein MG296", "uniref50_id": "UniRef50_P75364" }, "pscc": { "uniref50_id": "UniRef50_P75364", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75364" ], "product": "Uncharacterized protein MG296 homolog" }, "genes": [], "id": "KMADJIBOEG_689", "locus": "GCA000292445_00674" }, { "type": "cds", "sequence": "contig_1", "start": 364051, "stop": 364404, "strand": "-", "frame": 1, "gene": null, "product": "Cell division protein FTSY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PYR8" ], "nt": "TTGCAAAACAAGCTTAACTTAATGAATGAATTGCAAAAAATTTATCAAATTATTCAAAAGGTAAGTGGAAGTGAACCTAGTGAAACACTTTTAGTTTTAGATGGTACAGTAGGTCAAACAGGTTTATCACAAGCAAAGGTATTTAATGAATTTTCCAAACTAACAGGGATTGTTTTAACTAAAATGGATGGTTCTGCTAAGGGTGGAATTATTTTAGCTATTAAAGATATGTTTAACCTGCCTGTTAAACTGATAGGTTTTGGTGAAAAAACTAGTGATTTAGCTATCTTTGATCTGGAAAAATATGTTTTAGGTTTACTTAATAACTTAAACTTAGATAATAAAGAAAATTAG", "aa": "MQNKLNLMNELQKIYQIIQKVSGSEPSETLLVLDGTVGQTGLSQAKVFNEFSKLTGIVLTKMDGSAKGGIILAIKDMFNLPVKLIGFGEKTSDLAIFDLEKYVLGLLNNLNLDNKEN", "aa_hexdigest": "3ea25eb985dd7cc714a44d9a0d39f578", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PYR8", "query_cov": 0.9658119658119658, "subject_cov": 0.889763779527559, "identity": 0.655, "score": 149.0, "evalue": 3.42e-44, "valid": false, "product": "Cell division protein FTSY", "uniref50_id": "UniRef50_A0A3B0PYR8" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PYR8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PYR8" ], "product": "Cell division protein FTSY" }, "genes": [], "id": "KMADJIBOEG_690", "locus": "GCA000292445_00675" }, { "type": "cds", "sequence": "contig_1", "start": 364534, "stop": 365091, "strand": "-", "frame": 1, "gene": "ftsY", "product": "signal recognition particle-docking protein FtsY", "db_xrefs": [ "COG:COG0552", "COG:U", "EC:3.6.5.4", "GO:0003924", "GO:0005047", "GO:0005525", "GO:0005737", "GO:0005886", "GO:0006605", "GO:0006614", "GO:0009306", "GO:0016887", "KEGG:K03110", "SO:0001217", "UniRef:UniRef50_P47539", "UniRef:UniRef90_P47539" ], "nt": "ATGGGCTTTTTAAGCAAGTTAATTGCAAAACTAAAACCAAAAAAATCAGTTGCTAAACAGCTTAAAGAAGAAGTTGAAAAACAAAGCCTTTTTCAAACCAATAATAAAACTTACTATCAGGGTTTGAAAAAATCTGCTACAACTTTCGCTAAAACTATTAATGAACTGTCAAAACGATATGTTAATGTTGACGAACAGTTTAAAGAAAATCTATTTGAAGGGTTAGTTTTGCTTGATGTTGGTTATCATGCTGCAAACAAAATTTGTGATGCTATTATTGAACAGATCAAGCTAAACAGAATTACAGATTTTCAACTCATTAAAGAGCTAATTATTGACCAAATCATTGTTTATTACATCCAAGATAAACTCTTTGATACTGATTTAATAGTTAAACCTAACTTTACAAATGTTTATCTCTTTGTTGGTGTTAATGGAGTTGGTAAAACAACTACTTTAGCTAAGATAGCGGATTTTTTCATAAAACAAAATAAACGTGTTCTACTTGTTGCAGGTGATACTTTTAGAGCAGGAGCCATTGAACAACTTAATCAGTGA", "aa": "MGFLSKLIAKLKPKKSVAKQLKEEVEKQSLFQTNNKTYYQGLKKSATTFAKTINELSKRYVNVDEQFKENLFEGLVLLDVGYHAANKICDAIIEQIKLNRITDFQLIKELIIDQIIVYYIQDKLFDTDLIVKPNFTNVYLFVGVNGVGKTTTLAKIADFFIKQNKRVLLVAGDTFRAGAIEQLNQ", "aa_hexdigest": "3ce5e31761c7177dd696a15c2d827d00", "start_type": "ATG", "rbs_motif": null, "genes": [ "ftsY" ], "truncated": "5-prime", "pseudogene": { "start": 364231, "stop": 365091, "observations": { "mutation": [ 364536 ], "stop": [ 364536 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47539", "query_cov": 1.0, "subject_cov": 0.5346820809248555, "identity": 1.0, "score": 350.0, "evalue": 2.8e-119, "gene_start": 1, "gene_end": 185, "reference_start": 1, "reference_end": 185, "reference_sequence": "MGFLSKLIAKLKPKKSVAKQLKEEVEKQSLFQTNNKTYYQGLKKSATTFAKTINELSKRYVNVDEQFKENLFEGLVLLDVGYHAANKICDAIIEQIKLNRITDFQLIKELIIDQIIVYYIQDKLFDTDLIVKPNFTNVYLFVGVNGVGKTTTLAKIADFFIKQNKRVLLVAGDTFRAGAIEQLNQWAKLLNCDIVLPNPKEQTPAVIFRGVKKGIDDKYDFVLCDTSGRLQNKLNLMNELQKIYQIIQKVSGSEPSETLLVLDGTVGQTGLSQAKVFNEFSKLTGIVLTKMDGSAKGGIILAIKDMFNLPVKLIGFGEKTSDLAIFDLEKYVLGLLNNLNLDNKEN" }, "paralog": false, "identity": 0.9895104895104895, "score": 551.0, "evalue": 3.67e-201, "description": "Internal stop codon at 364536. Nonsense mutation around 364536.", "psc": { "uniref90_id": "UniRef90_P47539", "gene": "ftsY", "product": "signal recognition particle-docking protein FtsY", "ec_ids": [ "3.6.5.4" ], "uniref50_id": "UniRef50_P47539", "cog_id": "COG0552", "cog_category": "U", "kegg_orthology_id": "K03110", "go_ids": [ "GO:0003924", "GO:0005047", "GO:0005525", "GO:0005737", "GO:0005886", "GO:0006605", "GO:0006614", "GO:0009306", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47539", "EC:3.6.5.4", "UniRef:UniRef50_P47539", "COG:COG0552", "COG:U", "KEGG:K03110", "GO:0003924", "GO:0005047", "GO:0005525", "GO:0005737", "GO:0005886", "GO:0006605", "GO:0006614", "GO:0009306", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47539", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47539" ], "product": "Signal recognition particle receptor FtsY" } }, "id": "KMADJIBOEG_691", "locus": "GCA000292445_00676" }, { "type": "cds", "sequence": "contig_1", "start": 365098, "stop": 365934, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13304.12", "PFAM:PF02463.25" ], "nt": "TTGTTAGATGAACAACGTGCTAAATTAGTTTCAGCGCGGGAAGGAAAAATTAGGTTTGAAAATACAATCCAAAATATCACTGAAAAGATTAACAGTGTTTACAAAATGACAATGGAGTTTGCTATTGCTAATCACAACAAACCAGTTAAACTCTCTTTTATGCAAGCCCATAACAAGATTGCTAAACTACAAAATCAATTGAATGAAATGGGGGTAATTAACATGGAATCAATTGCTGAGATAAGTGAAAAGCAAAAGCGCTTTGATGATATTAACGCTGAATATGAATCAGCTCAACAAGCAGTTGAAAATTTGCAAAAAGCTATTACTGAAATTGATGAAATTGCTAGCAATGAGTTTGATCAACTGATCCAAAAATTAAATCAAGAGTTGCCAAAAACTTTCAAATATCTATTTGGTGGTGGTTCTTGCCAAATTCGTTACACTGATCCTAGTAATGTATTGGTTTCAGGGATAGATGTCTTTGCAAATCCTCCTGGTAAAAATATTGCCAACTTAATGTTGTTATCAGGCGGGGAAAAAACTTTAGTTGCACTTTCTGTTTTATTTAGTATTTTGAAAGTAAGTGCTTTTCCACTTGTTATTTTAGATGAAGCGGAAAGTGCACTTGATCCTGCTAATGTGGAGCGCTTTGCTAACATCATTAAAACTGCTAGCAAAAACACCCAATTTTTAATTATTACCCATCGTCAAGGTACAATGATGAAATGTGACATGTTATTGGGTGCTGCAATGCAAACTAAAGGTGTAACTAAAACCTTTGCAGTTGAACTTGAAAATGCTGAAAAATATGTATCTGAAAATGATTCTAATTAA", "aa": "MLDEQRAKLVSAREGKIRFENTIQNITEKINSVYKMTMEFAIANHNKPVKLSFMQAHNKIAKLQNQLNEMGVINMESIAEISEKQKRFDDINAEYESAQQAVENLQKAITEIDEIASNEFDQLIQKLNQELPKTFKYLFGGGSCQIRYTDPSNVLVSGIDVFANPPGKNIANLMLLSGGEKTLVALSVLFSILKVSAFPLVILDEAESALDPANVERFANIIKTASKNTQFLIITHRQGTMMKCDMLLGAAMQTKGVTKTFAVELENAEKYVSENDSN", "aa_hexdigest": "09903b3dddbef9970b7bd6035bba9206", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13304.12", "name": "AAA_21", "length": 193, "aa_cov": 0.6906474820143885, "hmm_cov": 0.8238341968911918, "evalue": 3.416821774766043e-10, "score": 35.55876922607422, "start": 45, "stop": 236 }, { "id": "PF02463.25", "name": "SMC_N", "length": 199, "aa_cov": 0.7158273381294964, "hmm_cov": 0.6432160804020101, "evalue": 1.8098800423870312e-29, "score": 97.8553237915039, "start": 60, "stop": 258 } ], "seq_stats": { "molecular_weight": 31030.26390000003, "isoelectric_point": 5.435876655578612 }, "id": "KMADJIBOEG_692", "locus": "GCA000292445_00677" }, { "type": "cds", "sequence": "contig_1", "start": 366043, "stop": 367377, "strand": "-", "frame": 1, "gene": "smc", "product": "chromosome segregation protein SMC", "db_xrefs": [ "COG:COG1196", "COG:D", "GO:0003677", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0007062", "GO:0016887", "GO:0030261", "KEGG:K03529", "SO:0001217", "UniRef:UniRef50_P47540", "UniRef:UniRef90_P47540" ], "nt": "TTGAAAGAGTTGGAACTTGCTGTTTTGGTTGGGGAATATCTCCAAGCACAAACTGAACTTGATAAGTTTAATTTTCAGATTAACAGTAGTGAACATGACTTTAAAATACACGAACCCCAACTAGAGTTGTTAGAAGAACAGATTGTTATCTTTAATAGTCGCTTTCATAGTGCTGATATGCAATCTAATGAATTGCAAAAAGAGTTACAAGATATTTACCAAAAGATCAATGAATTGGAACAACGTAAAGTCATTATTGATGTGCAGTTAAGACAAGGTTTTTCACAAAAAGATGAAAAGCAAAAAGCTGCAGCTTTAAAAAAACTAATTTTAGTTGATCAAACCCAGCTTGATGGTTTTGAAAACCAACTATCAAATTCCAAAACAACCATCACTGATCTTGAAAAACTAATTAACGAACAAAAATCACTTGTTGATCAGATAAAGTTACAGATTGAAAAAAATACTGCTGATTTAATTTACCAACGTTCCTTAAAAACAATTATTGAACTCCAAACTAATGAGCTTAAAAAAACTAACAATGCCAATATCTTAGTTAAAAATGCTAATGCTTTAACAGGAATTTTAAATACACTAGGAACATTTCTTAAATTTGATAAGCAGTATGAAAAAGCTATCTTAAAAGCACTTGGAAAATCAATTGGTTACCTAGTTGTTAACAACAATAATGCTGCCATCCAAGCAATAGATTTTTTAGTCAAAAATGAGATAGGTAAAGTAACTTTTTTACCTTTAGATGATGTTGCTAGTGATACTAAAATCACAAACGAACACATGGAAATTTTAAAGCAGCTTGATGGTTTTCTTGGTGTTTGTAGTGATCATGTTAAGTGTGATCCATTGTTTCAACCAGTTGTAAATACTCTTTTAGCACAAGTTATTATTGCTAAAGATTTAAATAGTGCCATTAACCTTTCTAACTATACTTACAAGTTATATAGGATCGTTACTTTAGATGGTGAGACAGTTTATGCAGGGGGAATTATCAATGGTGGATTTGAAAAAACAAATCTTAGTGATGGGTATTTATCATCAGCTTCACTTGATAATGAACAGAACATTAACAAACTAGAAAACAACGAACGAGAGTTAAAAAAAGAGCTTACAGAATTAGAAGTAAAGCTAGATGAAATGAACAGAAAACTCAAGTATGAAGAGTTGTTACAAGCCAAGTTTATAGAGCGTATTGTTCAAATTAAAAAAATTATTTTAGAGCTCAAAATGGAATATGAACAGCTTACTAACACAACTTTTGATGGTAAAAAAGCTGTTGCTAGTGAAGCTGAATTAATCCATTCATTAAATAGTGCTTGA", "aa": "MKELELAVLVGEYLQAQTELDKFNFQINSSEHDFKIHEPQLELLEEQIVIFNSRFHSADMQSNELQKELQDIYQKINELEQRKVIIDVQLRQGFSQKDEKQKAAALKKLILVDQTQLDGFENQLSNSKTTITDLEKLINEQKSLVDQIKLQIEKNTADLIYQRSLKTIIELQTNELKKTNNANILVKNANALTGILNTLGTFLKFDKQYEKAILKALGKSIGYLVVNNNNAAIQAIDFLVKNEIGKVTFLPLDDVASDTKITNEHMEILKQLDGFLGVCSDHVKCDPLFQPVVNTLLAQVIIAKDLNSAINLSNYTYKLYRIVTLDGETVYAGGIINGGFEKTNLSDGYLSSASLDNEQNINKLENNERELKKELTELEVKLDEMNRKLKYEELLQAKFIERIVQIKKIILELKMEYEQLTNTTFDGKKAVASEAELIHSLNSA", "aa_hexdigest": "795a2de1cb2bb1cdd200c0f7cea4c51a", "start_type": "TTG", "rbs_motif": null, "genes": [ "smc" ], "truncated": "both", "pseudogene": { "start": 365740, "stop": 367677, "observations": { "mutation": [ 366045, 367599 ], "stop": [ 366045, 367599 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47540", "query_cov": 1.0, "subject_cov": 0.45213849287169044, "identity": 0.998, "score": 763.0, "evalue": 3.12e-268, "gene_start": 1, "gene_end": 444, "reference_start": 224, "reference_end": 667, "reference_sequence": "MVFLKRFRAYGFKSYADEITIDFTHSMTGIVGPNGSGKSNVVDALKWVLGERSMKHLRSKSGDDMIFFGSKDKPASKLAEIELTFDNSNRLLHDSRKEISVMRRVYRGSGQSEYFINSNPATLKEISGIFADIGLEKGSLGIISQGSVSWFVEAKPEERRKIFEDASGIGRYTKRKEEVVNQLNRTLINLKQVSVVLNELKKDLKKLTLQAEKAQQFIRVKNELKELELAVLVGEYLQAQTELDKFNFQINSSEHDFKIHEPQLELLEEQIVIFNSRFHSADMQSNELQKELQDIYQKINELEQRKVIIDVQLRQGFSQKDEKQKAAALKKLILVDQTQLDGFENQLSNSKTTITDLEKLINEQKSLVDQIKLQIEKNTADLIYQRSLKTIIELQTNELKKTNNANILVKNANALTGILNTLGTFLKFDKQYEKAILKALGKSIGYLVVNNNNAAIQAIDFLVKNEIGKVTFLPLDDVASDTKITNEHMEILKQLDGFLGVCSDHVKCDPLFQPVVNTLLAQVIIAKDLNSAINLSNYTYKLYRIVTLDGETVYAGGIINGGFEKTNLSDGYLSSASLDNEQNINKLENNERELKKELTELEVKLDEMNRKLKYEELLQAKFIERIVQIKKIILELKMEYEQLTNTTFDGKKAVASEAELIHSLNSAWAKRDEINSKLKLNQELKLQLAKTIKQSEEKIVDLRALLDEQRAKLVSAREGKIRFENTIQNITEKINSVYKMTMEFAIANHNKPVKLSSMQAHNKIAKLQNQLNEMGVINMESIAEISEKQKRFDDINAEYESAQQAVENLQKAITEIDEIASNEFDQLIQKLNQELPKTFKYLFGGGSCQIRYTDPSNVLVSGIDVFANPPGKNIANLMLLSGGEKTLVALSVLFSILKVSAFPLVILDEAESALDPANVERFANIIKTASKNTQFLIITHRQGTMMKCDMLLGAAMQTKGVTKTFAVELENAEKYVSENDSN" }, "paralog": false, "identity": 0.9953488372093023, "score": 1153.0, "evalue": 0.0, "description": "Internal stop codon at 366045, 367599. Nonsense mutation around 366045, 367599.", "psc": { "uniref90_id": "UniRef90_P47540", "gene": "smc", "product": "chromosome segregation protein SMC", "uniref50_id": "UniRef50_P47540", "cog_id": "COG1196", "cog_category": "D", "kegg_orthology_id": "K03529", "go_ids": [ "GO:0003677", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0007062", "GO:0016887", "GO:0030261" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47540", "UniRef:UniRef50_P47540", "COG:COG1196", "COG:D", "KEGG:K03529", "GO:0003677", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0007062", "GO:0016887", "GO:0030261" ] }, "pscc": { "uniref50_id": "UniRef50_P47540", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47540" ], "product": "Chromosome partition protein Smc" } }, "id": "KMADJIBOEG_693", "locus": "GCA000292445_00678" }, { "type": "cds", "sequence": "contig_1", "start": 367394, "stop": 367732, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTGTTGTGCTTTTTCAGCTTGTAATGTAAGCTTTTTTAAATCTTTTTTCAGTTCATTTAAAACAACGCTAACTTGTTTTAAGTTAATTAAGGTTCGATTTAACTGGTTAACAACTTCTTCTTTACGCTTGGTATATCTACCAATTCCCGACGCATCTTCAAAGATCTTTCTGCGTTCTTCTGGCTTAGCTTCTACAAATCAAGAGACACTCCCTTGTGAAATAATTCCAAGTGAACCTTTTTCAAGACCAATATCAGCAAAAATACCGCTAATTTCTTTTAAAGTTGCTGGATTGGAGTTAATAAAATATTCACTCTGGCCACTCCCTCTATAA", "aa": "MNCCAFSACNVSFFKSFFSSFKTTLTCFKLIKVRFNWLTTSSLRLVYLPIPDASSKIFLRSSGLASTNQETLPCEIIPSEPFSRPISAKIPLISFKVAGLELIKYSLWPLPL", "aa_hexdigest": "e6ec10abafac792283f12dc0d48fefe9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12563.762599999984, "isoelectric_point": 9.46655368804932 }, "id": "KMADJIBOEG_694", "locus": "GCA000292445_00679" }, { "type": "cds", "sequence": "contig_1", "start": 367597, "stop": 368046, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13175.12", "PFAM:PF13304.12", "PFAM:PF13476.12", "PFAM:PF13555.12", "PFAM:PF02463.25" ], "nt": "ATGGTTTTTCTAAAACGTTTTCGCGCATATGGTTTTAAATCATATGCTGATGAAATCACAATTGATTTTACCCATTCAATGACTGGTATTGTTGGTCCTAATGGATCGGGCAAATCCAATGTTGTTGATGCATTAAAGTGGGTTTTAGGGGAGAGAAGTATGAAACATCTCCGTAGTAAATCAGGTGATGATATGATCTTTTTTGGCTCTAAAGACAAACCTGCTAGTAAGTTAGCTGAAATAGAGCTAACCTTTGATAATTCCAACCGTTTATTACATGATTCAAGAAAAGAGATAAGCGTGATGCGTAGGGTTTATAGAGGGAGTGGCCAGAGTGAATATTTTATTAACTCCAATCCAGCAACTTTAAAAGAAATTAGCGGTATTTTTGCTGATATTGGTCTTGAAAAAGGTTCACTTGGAATTATTTCACAAGGGAGTGTCTCTTGA", "aa": "MVFLKRFRAYGFKSYADEITIDFTHSMTGIVGPNGSGKSNVVDALKWVLGERSMKHLRSKSGDDMIFFGSKDKPASKLAEIELTFDNSNRLLHDSRKEISVMRRVYRGSGQSEYFINSNPATLKEISGIFADIGLEKGSLGIISQGSVS", "aa_hexdigest": "17ea2e276da776f1adc2da1b3e150dee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13175.12", "name": "AAA_15", "length": 83, "aa_cov": 0.5503355704697986, "hmm_cov": 0.9879518072289156, "evalue": 1.146481334715279e-11, "score": 40.01136016845703, "start": 11, "stop": 92 }, { "id": "PF13304.12", "name": "AAA_21", "length": 28, "aa_cov": 0.18791946308724833, "hmm_cov": 0.9285714285714286, "evalue": 3.389389096209681e-11, "score": 38.8541374206543, "start": 27, "stop": 54 }, { "id": "PF13476.12", "name": "AAA_23", "length": 120, "aa_cov": 0.7785234899328859, "hmm_cov": 0.9333333333333333, "evalue": 1.9277642999695324e-18, "score": 62.935909271240234, "start": 11, "stop": 126 }, { "id": "PF13555.12", "name": "AAA_29", "length": 37, "aa_cov": 0.24161073825503357, "hmm_cov": 0.972972972972973, "evalue": 2.240150600435118e-08, "score": 28.913469314575195, "start": 14, "stop": 49 }, { "id": "PF02463.25", "name": "SMC_N", "length": 137, "aa_cov": 0.912751677852349, "hmm_cov": 0.9854014598540146, "evalue": 8.942813133093844e-42, "score": 138.0916290283203, "start": 3, "stop": 138 } ], "seq_stats": { "molecular_weight": 16489.59029999998, "isoelectric_point": 9.360503196716309 }, "id": "KMADJIBOEG_695", "locus": "GCA000292445_00680" }, { "type": "cds", "sequence": "contig_1", "start": 368453, "stop": 369106, "strand": "-", "frame": 3, "gene": "pta", "product": "phosphate acetyltransferase", "db_xrefs": [ "COG:C", "COG:COG0280", "EC:2.3.1.8", "GO:0005737", "GO:0006085", "GO:0006086", "GO:0008959", "SO:0001217", "UniRef:UniRef50_P47541", "UniRef:UniRef90_P47541" ], "nt": "ATGCTTAATGAATCTAAGCTGATCCAACCTGCAGTTATCTTTCATAATCGTCAGGAAATCCCTGCAAATTTTGATAAAAAAATAACTCATTATGTGATTGATGAGATGGATTTAACTAGCTATGCTAACTTTGTCTATGAAAAACGTAAGCATAAGGGGATGGATTTAAAAGAAGCACAAAAGTTTGTACGTGATCCTAGTTCTTTAGCTGCTACCTTAGTTGCTCTAAAGGTTGTTGATGGTGAGGTTTGTGGTAAAGAATATGCTACAAAAGATACTTTAAGACCAGCTTTACAGTTACTAGCAACTGGTAATTTTGTTTCTAGTGTTTTCATCATGGAAAAAGGTGAAGAACGTTTGTACTTCACTGATTGTGCTTTTGCTGTTTATCCTAACCCCCAAGAGTTAGCAACAATTGCTGAAAACACCTTCAATTTTGCCAAAAGTTTAAATGAGGATGAGATAAAAATGGCTTTTTTAAGCTATTCAACGCTTGGCAGTGGTAAGGGTGAAATGGTGGATAAAGTTGTTTTAGCAACTAAACTATTTTTAGAAAAACACCCTGAATTGCATCAAAGTGTTTGTGGTGAGCTCCAGTTTGATGCTGCTTTTGTTGAAAAGGTTAGGTTACAAAAAGCACCTCAACTAACTTGA", "aa": "MLNESKLIQPAVIFHNRQEIPANFDKKITHYVIDEMDLTSYANFVYEKRKHKGMDLKEAQKFVRDPSSLAATLVALKVVDGEVCGKEYATKDTLRPALQLLATGNFVSSVFIMEKGEERLYFTDCAFAVYPNPQELATIAENTFNFAKSLNEDEIKMAFLSYSTLGSGKGEMVDKVVLATKLFLEKHPELHQSVCGELQFDAAFVEKVRLQKAPQLT", "aa_hexdigest": "a2508276b08614e1a06e8e22a53652c7", "start_type": "ATG", "rbs_motif": null, "genes": [ "pta" ], "truncated": "both", "pseudogene": { "start": 368249, "stop": 369211, "observations": { "mutation": [ 368455, 369136 ], "stop": [ 368455, 369136 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47541", "query_cov": 1.0, "subject_cov": 0.678125, "identity": 0.995, "score": 427.0, "evalue": 1.56e-149, "gene_start": 1, "gene_end": 217, "reference_start": 36, "reference_end": 252, "reference_sequence": "MSVIDIFKKRLQAVSKKPVIIFPEGWSASVLKAVEMLNESKLIQPAVIFHNRQEIPANFDKKITHYVIDEMDLTSYANFVYEKRKHKGMDLKEAQKFVRDPSSLAATLVALKVVDGEVCGKEYATKDTLRPALQLLATGNFVSSVFIMEKGEERLYFTDCAFAVYPNSQELATIAENTFNFAKSLNEDEIKMAFLSYSTLGSGKGEMVDKVVLATKLFLEKHPELHQSVCGELQFDAAFVEKVRLQKAPQLTWKNSANIYVFPNLDAGNIAYKIAQRLGGYDAIGPIVLGLSSPVNDLSRGASVSDIFNVGIITAAQAIK" }, "paralog": false, "identity": 0.990625, "score": 613.0, "evalue": 1.06e-225, "description": "Internal stop codon at 368455, 369136. Nonsense mutation around 368455, 369136.", "psc": { "uniref90_id": "UniRef90_P47541", "gene": "pta", "product": "phosphate acetyltransferase", "ec_ids": [ "2.3.1.8" ], "uniref50_id": "UniRef50_P47541", "cog_id": "COG0280", "cog_category": "C", "go_ids": [ "GO:0005737", "GO:0006085", "GO:0006086", "GO:0008959" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47541", "EC:2.3.1.8", "UniRef:UniRef50_P47541", "COG:COG0280", "COG:C", "GO:0005737", "GO:0006085", "GO:0006086", "GO:0008959" ] }, "pscc": { "uniref50_id": "UniRef50_P47541", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47541" ], "product": "Phosphate acetyltransferase" } }, "id": "KMADJIBOEG_696", "locus": "GCA000292445_00681" }, { "type": "cds", "sequence": "contig_1", "start": 369229, "stop": 369339, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACTATCTGATCAGTTTAGTTTTATCTCCACTGGTGGTGGTGCTTCTTTAGCACTAATTGGTGGGGAAGAGTTAGTAGGTATTAGCGATATTCAAAAAAATTCTTAA", "aa": "MQLSDQFSFISTGGGASLALIGGEELVGISDIQKNS", "aa_hexdigest": "72e6128bc3450b00e01137ee446d32a7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3671.0473, "isoelectric_point": 4.0500284194946286 }, "id": "KMADJIBOEG_697", "locus": "GCA000292445_00682" }, { "type": "cds", "sequence": "contig_1", "start": 369472, "stop": 370464, "strand": "-", "frame": 1, "gene": "pgk", "product": "Phosphoglycerate kinase", "db_xrefs": [ "COG:COG0126", "COG:G", "EC:2.7.2.3", "GO:0004618", "GO:0005524", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0043531", "KEGG:K00927", "SO:0001217", "UniRef:UniRef50_Q7WTU1", "UniRef:UniRef90_P47542" ], "nt": "ATGCTTAATTTCAAAACACTCCAAGCAATTGATTTTCAAAACAAAACCGTTGTTTTAAGAAGTGATTTTAATGTCCCAATGATCAATGGGGTTATTAGTGATAGTGAAAGAATTTTAGCTGGTTTGGATACTATTAAGTTCTTAGTTAAAAAGAACTGCAAGATAGTGCTACTATCACACCTTTCAAGGATTAAGAGTTTAGAAGATAAACTAAACAACAAAAAATCTTTAAAGCCAGTTGCTGAATTACTCCAACAACTCTTACCAACTGTAAAGGTTCAATTTTCTTGTAAAAACACTGGTGCTGAAGTTAAACAAAAAGTGCAAGCATTAGCATTCGGTGAAATCCTTCTCCTTGAAAACACTCGCTATTGTGATGTAAACGATAAAGGAGAAATTGTTAAATTAGAAAGTAAAAATGATCCTGAACTAGCGAAATTCTGGGCTAGTTTAGGGGAAATTTTTGTTAATGATGCATTTGGTACTGCCCATAGAAAACATGCTTCTAATGCAGGAATTGCAAAGTATGTTGCAAAATCCTGTATTGGGTTTTTAATGGAAAAAGAACTAAAGAACCTCTCTTACCTAATTCAAAGCCCACAAAAACCCTTTGTTGTTGTTTTGGGTGGTGCGAAAGTATCAGATAAACTAAAGGTAGTTGAAAACTTACTAAAACTTGCTGATAATATCTTAATTGGCGGAGGGATGGTAAATACCTTTCTTAAAGCAAAAGGCAAAGCTACTGCTAATTCCCTAGTTGAAAAAGAGTTAATTGATGTTGCTAAGCAAATCTTGGATAAAGATACTCATAATAAGATTGTGCTGGCAATTGATCAGGTAATGGGTTCTGAATTTAAAGATCAAGCTGGCATTACTTTAGATGTTAGTGACAAAATTCAAGAACAATATCAATCCTATATGTCTCTAGATGTTGGATCTAAAACAATTGCTTTATTTGAAAGTTATTTAAAAACAGCCAAAACTATCTTTTGA", "aa": "MLNFKTLQAIDFQNKTVVLRSDFNVPMINGVISDSERILAGLDTIKFLVKKNCKIVLLSHLSRIKSLEDKLNNKKSLKPVAELLQQLLPTVKVQFSCKNTGAEVKQKVQALAFGEILLLENTRYCDVNDKGEIVKLESKNDPELAKFWASLGEIFVNDAFGTAHRKHASNAGIAKYVAKSCIGFLMEKELKNLSYLIQSPQKPFVVVLGGAKVSDKLKVVENLLKLADNILIGGGMVNTFLKAKGKATANSLVEKELIDVAKQILDKDTHNKIVLAIDQVMGSEFKDQAGITLDVSDKIQEQYQSYMSLDVGSKTIALFESYLKTAKTIF", "aa_hexdigest": "bc68450ef6a465d6f7aba9b0158d1a58", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47542", "query_cov": 1.0, "subject_cov": 0.8029197080291971, "identity": 0.997, "score": 631.0, "evalue": 7.77e-227, "valid": true, "gene": "pgk", "product": "Phosphoglycerate kinase", "ec_ids": [ "2.7.2.3" ], "uniref50_id": "UniRef50_Q7WTU1", "cog_id": "COG0126", "cog_category": "G", "kegg_orthology_id": "K00927", "go_ids": [ "GO:0005829", "GO:0043531", "GO:0005524", "GO:0004618", "GO:0006094", "GO:0006096" ] }, "pscc": { "uniref50_id": "UniRef50_Q7WTU1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q7WTU1" ], "product": "Phosphoglycerate kinase" }, "genes": [ "pgk" ], "id": "KMADJIBOEG_698", "locus": "GCA000292445_00683" }, { "type": "cds", "sequence": "contig_1", "start": 370466, "stop": 371479, "strand": "-", "frame": 3, "gene": "gap", "product": "type I glyceraldehyde-3-phosphate dehydrogenase", "db_xrefs": [ "COG:COG0057", "COG:G", "EC:1.2.1.12", "GO:0004365", "GO:0005737", "GO:0006006", "GO:0006096", "GO:0006735", "GO:0009986", "GO:0016620", "GO:0044650", "GO:0050661", "GO:0051287", "GO:0140032", "RefSeq:WP_009885879.1", "SO:0001217", "UniParc:UPI000012AEC4", "UniRef:UniRef100_P47543", "UniRef:UniRef50_P47543", "UniRef:UniRef90_P47543" ], "nt": "ATGGCAGCAAAGAATAGAACCATTAAGGTTGCAATCAATGGTTTTGGAAGAATTGGAAGACTTGTTTTTCGTTCTCTTCTCAGTAAGGCAAATGTTGAAGTTGTAGCAATTAATGATTTGACCCAACCTGAAGTTTTAGCGCACCTGTTGAAATATGATTCAGCTCATGGTGAATTGAAAAGAAAGATTACTGTTAAACAAAACATCTTGCAAATTGATAGAAAAAAGGTTTATGTTTTTAGTGAAAAAGATCCCCAAAATTTACCTTGGGATGAACATGATATTGATGTAGTAATTGAATCAACTGGTAGGTTTGTAAGTGAAGAGGGTGCTTCTCTCCATTTAAAAGCAGGTGCTAAAAGAGTAATTATTTCCGCACCCGCTAAAGAAAAAACTATCAGGACAGTTGTTTACAATGTTAATCACAAAACCATTAGTAGCGATGATAAGATCATCTCAGCAGCTAGCTGTACTACTAACTGTTTAGCACCATTAGTTCATGTACTTGAAAAGAACTTTGGGATTGTTTATGGAACGATGCTAACAGTTCATGCATATACTGCAGATCAACGCTTACAAGATGCTCCTCATAATGACTTACGTCGTGCTCGTGCTGCAGCTGTTAACATTGTGCCAACAACAACAGGAGCAGCTAAAGCAATTGGGCTTGTTGTTCCAGAAGCAAATGGCAAACTTAATGGGATGTCACTCCGTGTTCCAGTGTTAACTGGTTCTATTGTAGAGTTAAGTGTTGTACTTGAAAAAAGTCCATCTGTTGAACAAGTAAATCAAGCCATGAAGCGATTTGCTTCCGCTTCTTTTAAATATTGTGAAGATCCTATTGTATCTAGCGATGTGGTAAGTTCTGAATATGGTTCAATTTTCGATTCTAAACTAACCAATATTGTTGAAGTTGATGGCATGAAACTTTATAAGGTGTATGCATGGTATGATAATGAATCTTCCTATGTACACCAACTAGTGAGAGTAGTTAGCTATTGTGCTAAGCTCTAA", "aa": "MAAKNRTIKVAINGFGRIGRLVFRSLLSKANVEVVAINDLTQPEVLAHLLKYDSAHGELKRKITVKQNILQIDRKKVYVFSEKDPQNLPWDEHDIDVVIESTGRFVSEEGASLHLKAGAKRVIISAPAKEKTIRTVVYNVNHKTISSDDKIISAASCTTNCLAPLVHVLEKNFGIVYGTMLTVHAYTADQRLQDAPHNDLRRARAAAVNIVPTTTGAAKAIGLVVPEANGKLNGMSLRVPVLTGSIVELSVVLEKSPSVEQVNQAMKRFASASFKYCEDPIVSSDVVSSEYGSIFDSKLTNIVEVDGMKLYKVYAWYDNESSYVHQLVRVVSYCAKL", "aa_hexdigest": "01db5001e72ea8a645150eda93526624", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012AEC4", "ncbi_nrp_id": "WP_009885879.1", "uniref100_id": "UniRef100_P47543", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012AEC4", "RefSeq:WP_009885879.1", "UniRef:UniRef100_P47543" ] }, "ips": { "uniref100_id": "UniRef100_P47543", "uniref90_id": "UniRef90_P47543" }, "psc": { "uniref90_id": "UniRef90_P47543", "gene": "gap", "product": "type I glyceraldehyde-3-phosphate dehydrogenase", "ec_ids": [ "1.2.1.12" ], "uniref50_id": "UniRef50_P47543", "cog_id": "COG0057", "cog_category": "G", "go_ids": [ "GO:0004365", "GO:0005737", "GO:0006006", "GO:0006096", "GO:0006735", "GO:0009986", "GO:0016620", "GO:0044650", "GO:0050661", "GO:0051287", "GO:0140032" ] }, "pscc": { "uniref50_id": "UniRef50_P47543", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47543" ], "product": "Glyceraldehyde-3-phosphate dehydrogenase" }, "genes": [ "gap" ], "id": "KMADJIBOEG_699", "locus": "GCA000292445_00684" }, { "type": "cds", "sequence": "contig_1", "start": 371624, "stop": 371956, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGCAGTTATATTTAAATTACTCCCAACTGTTAAAAGTGAAATTATAAGAATTAAACAAGCTCAAGCTACTAGAGGTTTTATCTATAACAAGTGTTCGTTTTTAAATCCTTTCAAGATTAAAACCCTTTTTATTCCCGTATTACTTTCAACAGTTAAAAAAACAGAAACAACAGCGTTTGCTCTACAAGCTAAAGGTTATGATTTAAATAACACTAACAGAACTCATTATCCTTTAAAATACAACTTATTAAACGGGGTATTTTTATTAGTGGGTTTACTTTTATTTAGTATTTTATTAATTGCTAATAACTGGAATTTAGTTTACTGA", "aa": "MLAVIFKLLPTVKSEIIRIKQAQATRGFIYNKCSFLNPFKIKTLFIPVLLSTVKKTETTAFALQAKGYDLNNTNRTHYPLKYNLLNGVFLLVGLLLFSILLIANNWNLVY", "aa_hexdigest": "49bee9ae139ee3bd1b583fedbdb8e75d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12565.911899999983, "isoelectric_point": 10.063337516784667 }, "id": "KMADJIBOEG_700", "locus": "GCA000292445_00685" }, { "type": "cds", "sequence": "contig_1", "start": 372134, "stop": 372277, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTATTTTTCTTGTGGTTTAATGTAATTGTTAACGGTTTTATCTTTTTACCAAATACTGCTTTAAGCGTTGATCAGAACCATAATTTTTTAGGAAGTTTTATCTATTCAGGTGGTAATAATTTTTGTGGTGTTAGCTGGTGA", "aa": "MLFFLWFNVIVNGFIFLPNTALSVDQNHNFLGSFIYSGGNNFCGVSW", "aa_hexdigest": "0df8aff762d2c049e842b83ef8884369", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5349.0592, "isoelectric_point": 5.060398292541502 }, "id": "KMADJIBOEG_701", "locus": "GCA000292445_00686" }, { "type": "cds", "sequence": "contig_1", "start": 372442, "stop": 373515, "strand": "-", "frame": 1, "gene": "ecfA2", "product": "Putative ABC transporter ATP-binding protein MG303", "db_xrefs": [ "COG:COG1122", "COG:PR", "GO:0005524", "GO:0016887", "GO:0042626", "GO:0043190", "RefSeq:WP_010869419.1", "SO:0001217", "UniParc:UPI00001395D4", "UniRef:UniRef100_P47545", "UniRef:UniRef50_P75356", "UniRef:UniRef90_P47545" ], "nt": "ATGCAAACACCTCAAATAAAACTAGATTTAATTGAACAAAAATACCATCTTCAAGCTCAAAAAGCGAATCTTAAACTAGCAAAATTAGATTCAAAAAAACTGTTTTCTTTTTATAAAAAGGTTGATAAAATCATTAATCCTTTTTACTTTTTAAACAGTAAAAAAAGCATTCCATTTTTTAATAGCAAATTACTGAACATTGAACAAGATCCATTGTTTTTTAACAATATTAGTGTCTTTGTCAATAAGCATAATGATGGTCAGATCATTAAATGTTGTTCAGGGGTAATAGAACCTAATAAAATTACAGTTATTTTTGGTGAAAGTGGTAGTGGTAAAACTACACTAATAAAACAGTTAGGTTTATTTGAAAAACCTAGTTTTGGCTATATAAATTGTGCTAATTATTGTTATTTTGCAAACCAATACCAACAAAAGATAACAAAAAACTTTAAGCAAAAAATTGGTTATATTTTGCAAAAAGCAGAAGATCAGTTTTTTTGTGATTCTATTTTAGAAGAAGTTTTAACAGGAGCAGTGAACCTTAAACTTTGTCATAAAAAAGATGTTAATTATGCCAAAAAATACCTTGATCTATGTGGATTGGAAAACATCCCGTTAATAAAAAATCCCATTGAATTAAGCGATGGACAGAAGAAGCGTTTGGCATTAGCTAGTGTGTTAGCAATGCAAGTTAAGTTTTTAATCCTTGATGAACCTACTGTTGGGTTAGATCAAATGGCGATTAGCAACTTAAGCAAAATGCTAGTAGCAATGAAACAAACAACAAGGATTGTTATTGTTAGCCATGATGTTGATTTTATCTTTGAAACTGCTGATAAAATCATCCATCTCCACCAGGGAAAGATAATTCATCAAACCACTGTTAATGATTTTTTTTCAAACACCAGTTGGTTGATGCAATATGGAATCACCCCTCCAGTAATTATCCAAGCAGTTAAGATGTTTAATGAAAAGGGAATAGCTTTTAAAAACCAAGCAGAAATTAAAAACCTTGATGATCTTATTAGTGAACTAAAAAACTTATTTTCATGCAAAAAACCAGTTTTTTAA", "aa": "MQTPQIKLDLIEQKYHLQAQKANLKLAKLDSKKLFSFYKKVDKIINPFYFLNSKKSIPFFNSKLLNIEQDPLFFNNISVFVNKHNDGQIIKCCSGVIEPNKITVIFGESGSGKTTLIKQLGLFEKPSFGYINCANYCYFANQYQQKITKNFKQKIGYILQKAEDQFFCDSILEEVLTGAVNLKLCHKKDVNYAKKYLDLCGLENIPLIKNPIELSDGQKKRLALASVLAMQVKFLILDEPTVGLDQMAISNLSKMLVAMKQTTRIVIVSHDVDFIFETADKIIHLHQGKIIHQTTVNDFFSNTSWLMQYGITPPVIIQAVKMFNEKGIAFKNQAEIKNLDDLISELKNLFSCKKPVF", "aa_hexdigest": "c997a1eeb2e0f5acd2b08de7ef39d05a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001395D4", "ncbi_nrp_id": "WP_010869419.1", "uniref100_id": "UniRef100_P47545", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001395D4", "RefSeq:WP_010869419.1", "UniRef:UniRef100_P47545" ] }, "ips": { "uniref100_id": "UniRef100_P47545", "uniref90_id": "UniRef90_P47545" }, "psc": { "uniref90_id": "UniRef90_P47545", "gene": "EcfA2", "product": "Putative ABC transporter ATP-binding protein MG303", "uniref50_id": "UniRef50_P75356", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_P75356", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75356" ], "product": "Putative ABC transporter ATP-binding protein MG303 homolog" }, "genes": [ "ecfA2" ], "id": "KMADJIBOEG_702", "locus": "GCA000292445_00687" }, { "type": "cds", "sequence": "contig_1", "start": 373497, "stop": 374309, "strand": "-", "frame": 2, "gene": "ecfA2", "product": "Putative ABC transporter ATP-binding protein MG304", "db_xrefs": [ "COG:COG1122", "COG:PR", "GO:0005524", "GO:0016887", "GO:0042626", "GO:0043190", "RefSeq:WP_009885882.1", "SO:0001217", "UniParc:UPI0000557DFF", "UniRef:UniRef100_P47546", "UniRef:UniRef50_P47546", "UniRef:UniRef90_P47546" ], "nt": "TTGCTACAAGTTAAAAACCTCAGTTTTAAATACCCAAAACACCAAAACAATGTGTTAAATCAGATTAGTTTTAATGTTCAAGATGGGTGTCATTTAGCAGTAATAGGTCATAACGGTTCAGGTAAATCAACCCTAGTTAAACTCCTTGGCGGATTTCTAAAAGCTAAAAAAGGAACTATCTTTTTCAATGATAAGGAGTTGACTTCTGTTGGTTTTAATAAGATTGGAATCTTATTACAAGACCCTGATGTGCAATTACTTGCTGATACACTCCACCAGGAATTGATTTTTACTTTGGAAAACCATGGAGTTTTAGCTAGTGAAATGGATAAGATTATTAATGAAGTTTTAACTGTTGTTGAATTGAAAGATAAACAATTTACACCACTTAAAAAACTTTCATTTGGTGAAAAACAACGTGCTGTTTTTGCTTGTTTATTGGCTGTCAAACCACAAATTTATCTGCTTGATGAGGCATTTAGTATGCTTGATAATAAGATCAGCAATAAACTTAAAAAATTTGTGTTTGATACCATCAAAAAACAAAACAAAATAGTTATCAATATTACCCATGATTTTAATGATCTGTTTCTAGCAGATGAGATTATCTTTTTAAGTAAAGGATCGCTAATTAAAAAGTTTGCTCCTGAAGCGATATATGAGCAGCTTGATTTGTTTCATAACCACCACTTTAATCTCCCTTTTCCACTTTTATTGGCCCATAAGGTGGCTAAACAAATAAACAAAAAAACACCAAGTTTAAGTTTTGAATTGAATGATGTAGTTAACTGGATATGCAAACACCTCAAATAA", "aa": "MLQVKNLSFKYPKHQNNVLNQISFNVQDGCHLAVIGHNGSGKSTLVKLLGGFLKAKKGTIFFNDKELTSVGFNKIGILLQDPDVQLLADTLHQELIFTLENHGVLASEMDKIINEVLTVVELKDKQFTPLKKLSFGEKQRAVFACLLAVKPQIYLLDEAFSMLDNKISNKLKKFVFDTIKKQNKIVINITHDFNDLFLADEIIFLSKGSLIKKFAPEAIYEQLDLFHNHHFNLPFPLLLAHKVAKQINKKTPSLSFELNDVVNWICKHLK", "aa_hexdigest": "4d033138b35b5fe289f313b2d8a63aef", "start_type": "TTG", "rbs_motif": "ATTTA", "ups": { "uniparc_id": "UPI0000557DFF", "ncbi_nrp_id": "WP_009885882.1", "uniref100_id": "UniRef100_P47546", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000557DFF", "RefSeq:WP_009885882.1", "UniRef:UniRef100_P47546" ] }, "ips": { "uniref100_id": "UniRef100_P47546", "uniref90_id": "UniRef90_P47546" }, "psc": { "uniref90_id": "UniRef90_P47546", "gene": "EcfA2", "product": "Putative ABC transporter ATP-binding protein MG304", "uniref50_id": "UniRef50_P47546", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_P47546", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47546" ], "product": "Putative ABC transporter ATP-binding protein MG304" }, "genes": [ "ecfA2" ], "id": "KMADJIBOEG_703", "locus": "GCA000292445_00688" }, { "type": "cds", "sequence": "contig_1", "start": 374495, "stop": 376222, "strand": "-", "frame": 3, "gene": "dnaK", "product": "molecular chaperone DnaK", "db_xrefs": [ "COG:COG0443", "COG:O", "GO:0005524", "GO:0006457", "GO:0051082", "GO:0140662", "SO:0001217", "UniRef:UniRef50_P9WMJ9", "UniRef:UniRef90_P47547" ], "nt": "ATGAGTGCAGACAATGGTTTAATTATTGGCATTGACCTTGGAACTACCAATTCTTGTGTTTCTGTAATGGAAGGTGGGAGACCTGTTGTATTAGAAAATCCTGAAGGTAAAAGAACAACACCTTCCATTGTTTCCTATAAAAACAATGAAATTATAGTAGGTGATGCTGCTAAAAGACAGATGGTTACAAACCCAAATACCATTGTCTCCATCAAGAGGTTGATGGGTACCTCAAATAAAGTAAAAGTCCAAAATGCTGATGGTACAACTAAGGAATTAAGTCCTGAACAAGTTTCAGCGCAAATCCTTAGTTATCTTAAGGACTTTGCTGAAAAAAAGATTGGTAAAAAGATTTCAAGAGCAGTTATTACTGTTCCTGCATACTTTAATGATGCGGAAAGAAACGCTACTAAAACCGCAGGTAAGATTGCTGGTTTAAATGTTGAAAGGATCATTAACGAACCAACTGCTGCTGCTTTAGCTTATGGGATTGATAAAGCATCAAGAGAGATGAAAGTCTTGGTTTATGACTTGGGTGGTGGAACTTTTGATGTATCTTTACTTGACATTGCAGAAGGTACTTTTGAAGTACTTGCAACTGCTGGGGACAACCGTTTGGGAGGTGATGATTGGGATAACAAGATCATTGAATATATCTCAGCCTACATTGCCAAAGAACACCAGGGTTTAAACTTATCAAAAGATAAGATGGCAATGCAACGGCTTAAAGAAGCAGCTGAACGTGCTAAGATTGAACTTTCCGCTCAACTTGAAACGATTATTTCTTTACCATTTTTAACTGTTACCCAAAAAGGTCCTGTTAACGTTGAGTTAAAACTAACCCGTGCTAAGTTTGAGGAGTTAACAAAACCACTACTTGAAAGAACAAGAAACCCTATTTCAGATGTTATCAAGGAAGCTAAGATTAAACCTGAAGAGATTAATGAAATTCTTTTAGTTGGTGGTTCTACAAGGATGCCTGCAGTTCAAAAGCTAGTTGAATCAATGGTACCAGGTAAAAAACCAAACCGTTCTATTAATCCTGATGAAGTTGTTGCTATTGGCGCTGCTATTCAAGGTGGGGTTTTACGTGGTGATGTTAAGGATGTTTTACTTTTAGATGTAACTCCATTAACCCTTTCTATTGAAACTTTAGGTGGTGTGGCTACTCCTTTAATTAAGAGAAATACAACTATCCCAGTAAGTAAAAGTCAAATCTTTTCAACTGCTCAAGATAACCAAGAATCAGTTGATGTGGTTGTATGTCAAGGGGAAAGACCAATGTCTAGAGATAATAAGTCATTAGGAAGATTTAACTTAGGCGGTATTCAACCAGCACCTAAAGGTAAACCCCAAATTGAGATTACTTTTAGTTTGGATGCCAATGGGATCTTAAATGTTAAAGCTAAGGATTTAACCACGCAAAAGGAAAACAGTATTACCATTAGTGACAACGGTAATCTTTCTGAGGAGGAGATCCAAAAGATGATCCGTGATGCTGAAGCTAACAAGGAACGGGATAACATCATCCGTGAACGTATTGAATTACGTAATGAAGGGGAAGGTATTGTTAATACCATCAAAGAGATATTAGCAAGTCCTGATGCTAAGAATCTCCCTAAAGAAGAAAAAGAGAAGTTAGAAAAGCTAACAGGTAACATTGATGCTGCTATTAAAGCTAATGACTATGCCAAACTCAAAGTGGAAATTGAAAACTTTAAGAAGTGA", "aa": "MSADNGLIIGIDLGTTNSCVSVMEGGRPVVLENPEGKRTTPSIVSYKNNEIIVGDAAKRQMVTNPNTIVSIKRLMGTSNKVKVQNADGTTKELSPEQVSAQILSYLKDFAEKKIGKKISRAVITVPAYFNDAERNATKTAGKIAGLNVERIINEPTAAALAYGIDKASREMKVLVYDLGGGTFDVSLLDIAEGTFEVLATAGDNRLGGDDWDNKIIEYISAYIAKEHQGLNLSKDKMAMQRLKEAAERAKIELSAQLETIISLPFLTVTQKGPVNVELKLTRAKFEELTKPLLERTRNPISDVIKEAKIKPEEINEILLVGGSTRMPAVQKLVESMVPGKKPNRSINPDEVVAIGAAIQGGVLRGDVKDVLLLDVTPLTLSIETLGGVATPLIKRNTTIPVSKSQIFSTAQDNQESVDVVVCQGERPMSRDNKSLGRFNLGGIQPAPKGKPQIEITFSLDANGILNVKAKDLTTQKENSITISDNGNLSEEEIQKMIRDAEANKERDNIIRERIELRNEGEGIVNTIKEILASPDAKNLPKEEKEKLEKLTGNIDAAIKANDYAKLKVEIENFKK", "aa_hexdigest": "5c65c74fa307ad5c2e829b547fa48601", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47547", "query_cov": 1.0, "subject_cov": 0.9663865546218487, "identity": 0.998, "score": 1080.0, "evalue": 0.0, "valid": true, "gene": "dnaK", "product": "molecular chaperone DnaK", "uniref50_id": "UniRef50_P9WMJ9", "cog_id": "COG0443", "cog_category": "O", "go_ids": [ "GO:0005524", "GO:0006457", "GO:0051082", "GO:0140662" ] }, "pscc": { "uniref50_id": "UniRef50_P9WMJ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P9WMJ9" ], "product": "Chaperone protein DnaK" }, "genes": [ "dnaK" ], "id": "KMADJIBOEG_704", "locus": "GCA000292445_00689" }, { "type": "cds", "sequence": "contig_1", "start": 376459, "stop": 376722, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGTAGCAATTGTTTCCTACTTTAGTTTGTTTGGTGTTTTTATGATCACAAAAACTGTCAACACAACTAATAACGTTATTGATCAATACAGCTTAATTGATCTTTTTAACATTACTAATATTGCTGTTACCGCTTCTAACTTTATTCCAGTTGCTAGTGGGGAAGGTGCAACACAATTTGTGATGACTAGTTTTCTCAATGCATTCAAACCAACTGATCAATTCCTCCATGATCAGATTAAAGATGGTGTTTTTTTATGA", "aa": "MVVAIVSYFSLFGVFMITKTVNTTNNVIDQYSLIDLFNITNIAVTASNFIPVASGEGATQFVMTSFLNAFKPTDQFLHDQIKDGVFL", "aa_hexdigest": "0e818fcc072ad2cf1ab783cceb27ea3d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9603.948199999993, "isoelectric_point": 4.451254844665528 }, "id": "KMADJIBOEG_705", "locus": "GCA000292445_00690" }, { "type": "cds", "sequence": "contig_1", "start": 377218, "stop": 377490, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAGTTAACCACCAATACCTTTTTTAACACTAAAAACATCGTTGCTTTTAGCTTTTTTTTGGTTTTTCTAATCGTTATTAGTGTTATTGTTACTATCTTTTTTTTAGGTGTTAGTGTTGATGATGTGAAAACTATTATCACTGCAATTAACTATCAGAATTGGGGTTGGATATTTGTTGTTATCTTAGGGTTTTTAGTTTCAGTTTTGTGGAATGTAATCATTAATTGGTGGGTATCAAGGAGATTTTGTTTTTATGCTAGTTGGTGA", "aa": "MAKLTTNTFFNTKNIVAFSFFLVFLIVISVIVTIFFLGVSVDDVKTIITAINYQNWGWIFVVILGFLVSVLWNVIINWWVSRRFCFYASW", "aa_hexdigest": "da1c4f127275045829134d49d3f9934c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10539.441099999998, "isoelectric_point": 9.514002418518068 }, "id": "KMADJIBOEG_706", "locus": "GCA000292445_00691" }, { "type": "cds", "sequence": "contig_1", "start": 377490, "stop": 378155, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAGCACCAGACAAGCAGTATCAACAAGTAATAATGGTACTTATGGTTTTACTGGTTTGAATACAACTAACAGTTCCATGTTAGAAAACAATACTAACCAGGCACTTTTAAACCATATCGCTGCCAATTCCTTAAAACAATATGGTTCAAAGGATGATCTTAAAAAATTTATTAGTGAAACAAAAGACCAATTAGTTTTAGATAACATTGCCCGACAGCTAAGTAGACTAACTCCAAGTAGTAGTAGTAGTAGTAATGGCAAAACACTTTTAGCTTATTTTCAGGTCGATGCAATTAACAATTCTGCTTTAGATTTCAAAGCTAAACAAGCGTTGTTATTAGCTGTTTTAGACCAATATTCCAGTTATTTTAATTCATCAAATAGTCCAAGTATTTCAAAAAGAAGTACCCAAACCAACCAAAAATTTAGTGAATTTAACTTTGGTGGTGATAGTTACAACTATCTTCAGTTCACTAAAAGTGACATTGATTCACTTAGTTTAACAAGTTCTACCAATATTGAAAGTGATATAGTAGCAGCTTTAGTTTTATTTCAAGCTAGTGATACAGGCACCCAACAACTTGCTTTAAGTGCAATTGAAAAACCCCAATTTAGGATTGGTGATAAAAGAATTCAATCAGGCTTAAACTTGCTTAAGTAA", "aa": "MLSTRQAVSTSNNGTYGFTGLNTTNSSMLENNTNQALLNHIAANSLKQYGSKDDLKKFISETKDQLVLDNIARQLSRLTPSSSSSSNGKTLLAYFQVDAINNSALDFKAKQALLLAVLDQYSSYFNSSNSPSISKRSTQTNQKFSEFNFGGDSYNYLQFTKSDIDSLSLTSSTNIESDIVAALVLFQASDTGTQQLALSAIEKPQFRIGDKRIQSGLNLLK", "aa_hexdigest": "ee2a76a60f5543e0a90d0f96b1f34824", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24157.531799999997, "isoelectric_point": 8.707374000549319 }, "id": "KMADJIBOEG_707", "locus": "GCA000292445_00692" }, { "type": "cds", "sequence": "contig_1", "start": 378356, "stop": 378544, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGGTAATCCTAATAATGGCAATATTGATGAATTGTTTAAAAAAGCTTTTTTAGAGAGTGTTGATGAATCAGGTTTTAATGTTAACTTTAAAGCAGTTATTGACCATTATCGCTTCATTTTTACTTTGCAATGGTTAATTGAAAACAACCTAAAAACTTTAAAGATATTTTACAAGCTAACTTAA", "aa": "MYGNPNNGNIDELFKKAFLESVDESGFNVNFKAVIDHYRFIFTLQWLIENNLKTLKIFYKLT", "aa_hexdigest": "d343ceabc402a90d04d4454377c9a26a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7363.359799999996, "isoelectric_point": 6.538212013244629 }, "id": "KMADJIBOEG_708", "locus": "GCA000292445_00693" }, { "type": "cds", "sequence": "contig_1", "start": 378566, "stop": 379273, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAAGCATTCAATGATTACAAAGAACTAATGCAAAAATTTGCACCAGCTGCATTAGCAACAAATGTAATCAGAGATTTGTTTTTACAAATGGATGCATTAGATAACAAACTAACAAGTAGAACAACTGAACTTAATACCAATGTTAACCAAATTAATCCAACCCCTTGGTTAAATGGCTTATCTTCCCATTTGCCATATGTTCGCAAGTCTGGTCATTATGAAAAACTCAATAACTATTTCTTGTTTTTAATTACTAAAACACTTTGGAAAAAAGTAGGTAGTGAAAAAATAAATATTAGTGAAAACAACAATAAGTTAAAAACTACAAAAGCTGATGTTGATAAGATAAGAGATGAAATTCTGAGCGACATAAATAACAAAGTAACAGAATTTGTTAACCAATTGAAAGTTACAGAAAAATCAAAACCTAACTTTAGCAACATTATTCTAGTTGATATTAACAATGATCAATCACTTACTAACAGTGCCAATTGGAGTTTAAATGCGTTGTTAGATGTAACAACTGTAAACCCTCTTTCCTTTGCATTGTTAAAAAATGCTTTTACTAGCAATCAACAGTTTGAAAAAGCTAAAAAGTTATTTGAAGAGATTAAATCTAAAAATGGATCTTCATCAACTTCATCAAGTAGTGATGCAGATAGTTTAGCTAAAGTAATTAGCAATTACTATTACATGACATGA", "aa": "MQKAFNDYKELMQKFAPAALATNVIRDLFLQMDALDNKLTSRTTELNTNVNQINPTPWLNGLSSHLPYVRKSGHYEKLNNYFLFLITKTLWKKVGSEKINISENNNKLKTTKADVDKIRDEILSDINNKVTEFVNQLKVTEKSKPNFSNIILVDINNDQSLTNSANWSLNALLDVTTVNPLSFALLKNAFTSNQQFEKAKKLFEEIKSKNGSSSTSSSSDADSLAKVISNYYYMT", "aa_hexdigest": "2258203d0da5529e94bad90b25e537c2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26647.869700000025, "isoelectric_point": 9.155235862731931 }, "id": "KMADJIBOEG_709", "locus": "GCA000292445_00694" }, { "type": "cds", "sequence": "contig_1", "start": 379439, "stop": 379870, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCTGTGAACACAATACAACAGACAGTTCACAAAACAATTTAAAGAGTCTTATTAAAGATGCTTTTATAAGTTCTAATGATAGTTCCAATTTAGGAAAACTAGCAAAGCAAATCCACCAAACAACTACAAGTGATATGATGGTTTCAACTAAAGCTAGTTCATCAACAACAAGCAGTTATCTTGTTTGGGATGCAGCTATTCCTAATAACAAAACAAATAATGGTGCTAGCACTGTTAGTGCAAACTGTGCAAATGCTACTGTTCAAAATACAAGCCATAACTCTAATAACCAATTGAAGCTAAGACTAGTTAGAAACGGAGAGGGAGTTGCTGTAATTGGAATTGATGGAGGTAGTTATTATCTGACAAAAAATAGTAGTAGCAAAACTGAAAGAGACATTGAAAAGCAAAAGCAGTTTTTAATGTGA", "aa": "MFCEHNTTDSSQNNLKSLIKDAFISSNDSSNLGKLAKQIHQTTTSDMMVSTKASSSTTSSYLVWDAAIPNNKTNNGASTVSANCANATVQNTSHNSNNQLKLRLVRNGEGVAVIGIDGGSYYLTKNSSSKTERDIEKQKQFLM", "aa_hexdigest": "13139f6c43c45cbaa3b7401ad532b5c7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15470.92739999998, "isoelectric_point": 8.928565025329593 }, "id": "KMADJIBOEG_710", "locus": "GCA000292445_00695" }, { "type": "cds", "sequence": "contig_1", "start": 380192, "stop": 380416, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGCAAAGTTACAAGCACAAATCTTTAATCTCTGGGTTGAATATACCGATCCTACTTTAATCAGTCAAGCTACTTTTAAATATGCAGCACCACAACAAGGTTTGGGTCAAGTTTACAACACTGAAAAACTAAAAGATAAATTAACCCCTTCTTATGCGTTTCCTTTCTTTCCTAAAGATGGGGAAATTCCTCAGAACCAGAATGTTGGCAACAAACGCTGA", "aa": "MFAKLQAQIFNLWVEYTDPTLISQATFKYAAPQQGLGQVYNTEKLKDKLTPSYAFPFFPKDGEIPQNQNVGNKR", "aa_hexdigest": "e9a883727d7d1cffa9a817c3bf33fddb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8485.5487, "isoelectric_point": 9.004250907897951 }, "id": "KMADJIBOEG_711", "locus": "GCA000292445_00696" }, { "type": "cds", "sequence": "contig_1", "start": 380606, "stop": 381025, "strand": "-", "frame": 3, "gene": null, "product": "putative lipoprotein MPN_442", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75336" ], "nt": "ATGAAAAAACTTTTAAAAAAATCAAAGTTCTGGTGGTTTTTACTTTGTGGTTTGTCAGTAAGCACAATACTTGTAGCTTGTTCAACTCCAACAAACAGTGCTTTGCAAACAGTTTTTTCACCAACCTCAACTAATTTTTTTCATGGTCAAAAGGGTAGTTTAAAAGAAGGACTTATTACTGCTTTAAAAACTCCTTCAGCAAACAAACATTTTGTTATAGGACCACTTTTAAAAGCACTTGAGGCTTGGTATGAAAACAATGAAGACAAAGCCATAAACAAGTTTTTAAAAGACACTAAAAAGAATGTTGATGATCAATATAAAAATACTGTTAATGGGTTAATATCACCACCCAGAAACAGAGCAGTATTTATTCAACAAGATCTATTAGATAAAAGTGGTGGCAGTGAGGCTAGTTGA", "aa": "MKKLLKKSKFWWFLLCGLSVSTILVACSTPTNSALQTVFSPTSTNFFHGQKGSLKEGLITALKTPSANKHFVIGPLLKALEAWYENNEDKAINKFLKDTKKNVDDQYKNTVNGLISPPRNRAVFIQQDLLDKSGGSEAS", "aa_hexdigest": "10e6f857f9c6bac6b02964ccfe5aeb4d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75336", "query_cov": 0.9784172661870504, "subject_cov": 0.9006622516556292, "identity": 0.603, "score": 159.0, "evalue": 9.37e-48, "valid": false, "product": "Uncharacterized lipoprotein MPN_442", "uniref50_id": "UniRef50_P75336", "go_ids": [ "GO:0098552" ] }, "pscc": { "uniref50_id": "UniRef50_P75336", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75336" ], "product": "Uncharacterized lipoprotein MPN_442" }, "genes": [], "id": "KMADJIBOEG_712", "locus": "GCA000292445_00697" }, { "type": "cds", "sequence": "contig_1", "start": 381022, "stop": 381198, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGTTTTTGACTGAAAATAACCAAAAAACTACCAAAAAACCGCTTTCAACTTTACAAATTAAGAAAATTAAAGCTATCAAAGCTAAATATAAAAAGGTTAAACCTAACTACAAAAAATACCAAAAACAACAGATTCAAAACCTTTTAATTAATAAGAAAAAACCAAGATCATGA", "aa": "MKFLTENNQKTTKKPLSTLQIKKIKAIKAKYKKVKPNYKKYQKQQIQNLLINKKKPRS", "aa_hexdigest": "db354a7ab134bfb140c51409a315f68c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6960.372600000004, "isoelectric_point": 10.623052024841307 }, "id": "KMADJIBOEG_713", "locus": "GCA000292445_00698" }, { "type": "cds", "sequence": "contig_1", "start": 381370, "stop": 381786, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00271.37" ], "nt": "TTGTTTGCCTTTTTTAGTGCTACTTTATTCAATCAACAACTCCAAATAATTAAAAAACAAGTAATTAATACTAAAGTTATTAATCTCCATCCAAAGCAATGGATTCACCCCTTAGTTAAGCATTTTGTTGTTCATCTCAATACTGAAAACCGTTTTTCTGGTTTATTAGCCTTATTAAAACACCACCAAAATCAGCAAATTATTGTTTTCTGTTCTAATCAAAAATCTTTAAAACAATTAACACAATTGCTTTCAAATAACAATATTAGTTTTGGTTCAATTTATGGTAGTTTAACCTATCAAGAACGTAAAAATAATTTCACAAAAGCAACTAATAATAAATTGAAATTACTAGTTGTTTCTGATCTATTTTCACGTGGGATAGATTTAAATTATTTCAGTGTGGTGATTTCTTGA", "aa": "MFAFFSATLFNQQLQIIKKQVINTKVINLHPKQWIHPLVKHFVVHLNTENRFSGLLALLKHHQNQQIIVFCSNQKSLKQLTQLLSNNNISFGSIYGSLTYQERKNNFTKATNNKLKLLVVSDLFSRGIDLNYFSVVIS", "aa_hexdigest": "7e2a69c49f7eff7cbea2f7755ce40229", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00271.37", "name": "Helicase_C", "length": 85, "aa_cov": 0.6159420289855072, "hmm_cov": 1.0, "evalue": 1.2940416803884505e-17, "score": 59.229312896728516, "start": 53, "stop": 137 } ], "seq_stats": { "molecular_weight": 15959.388299999986, "isoelectric_point": 10.178349113464353 }, "id": "KMADJIBOEG_714", "locus": "GCA000292445_00699" }, { "type": "cds", "sequence": "contig_1", "start": 382264, "stop": 383073, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAAAGTGCACAGCAAACCAATAACCAGCAAAGAAGGTTTGGTTTCCATGGTATTGTTACCAACACTTCATCCAACAACTTACCAGATGCAGTTAGAAATCGCCTCTTTACAAGTTTTGTTTCCCAATCTGAAAAATCAAGTTCTAATGGTGGTCAAGCACAATTACAATCTACTCAATCAAGTGGTAGTAATGAAACAATTTACAAAGGTGCTTTATTTAGTTTTGGTTCTCTAACAAAACTTATAGAAACAATTGATAACATCCCAACCCAAGCTGAGTTTGATGCACTTTATAACCATTTAACAAGTAACCTTAATATTAACGTTACTGGTGTTGATAGATCAAAATCCTTACAAGAACAAAAAACTAATTTGAAGAATTTTGCTAATTCAAACTTTAACAATACTCAAACTGTTCAATTAAAACAAGCTCAAAGTAAAACTAATAATAGTAACTTTAATGATGTGTTTAGTAGGTTTGAAGGGTATATAGGTACAAATAAAACCAGTAATTATAGCAGTTATAACTTTTTACAAGATAACCAGATCTATCATGCAGTTTATGCAAAACAGATTAACCTTGAAGATGTAAGTATGCTTGGTAGTGATAGTTTGAATAGTACAGATTCAAATAATTCAAAAAGACTTGATCTTTCACTTGAAGAATTTTTAAGTACAGTTGCGCTTGAAGCGCTTAATCCTAATAATCAAACCCAAGCTATTAATGCATTGATAGCAAATGCCAAAAATGGCTTAGTTAGAGTTGGTGATAACAGATTGTTTTCAGCAATCAGTTCACAGTGA", "aa": "MQKSAQQTNNQQRRFGFHGIVTNTSSNNLPDAVRNRLFTSFVSQSEKSSSNGGQAQLQSTQSSGSNETIYKGALFSFGSLTKLIETIDNIPTQAEFDALYNHLTSNLNINVTGVDRSKSLQEQKTNLKNFANSNFNNTQTVQLKQAQSKTNNSNFNDVFSRFEGYIGTNKTSNYSSYNFLQDNQIYHAVYAKQINLEDVSMLGSDSLNSTDSNNSKRLDLSLEEFLSTVALEALNPNNQTQAINALIANAKNGLVRVGDNRLFSAISSQ", "aa_hexdigest": "5210017a7e8bff392c49153ed5b0fd3c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29728.128400000034, "isoelectric_point": 7.891591072082519 }, "id": "KMADJIBOEG_715", "locus": "GCA000292445_00700" }, { "type": "cds", "sequence": "contig_1", "start": 383173, "stop": 383304, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGTAAGTTCTTCTAAATCTGTATTAGTAAAAATGGCTAACAACGTTGCTTCTCAAACTAATCAAGACTTTACCAAGCAAGAACAACAAAATCCTAACTATGTATTTGGTTCGAGTGCTTATAATTGA", "aa": "MSVSSSKSVLVKMANNVASQTNQDFTKQEQQNPNYVFGSSAYN", "aa_hexdigest": "434ac94a7d5d89db34df5fe6aa1e22bd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4730.1202, "isoelectric_point": 8.1392786026001 }, "id": "KMADJIBOEG_716", "locus": "GCA000292445_00701" }, { "type": "cds", "sequence": "contig_1", "start": 383665, "stop": 384981, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAAAGCTAGTGATATGTTTGATACCTTTGATCCTAGCTTTTCAGCAGCTTTTATCCAGGGCTATTTAGCTTTACAAAAAAAATCCAAAGGTGCAGAACAAACTGAATACACTAAATTAGAAAAAGATAAATCAATCATTGAAAATTTCTTTGTTGAAAATAATTCAGCTAAGGCTGCAATGAAAAGTGCAAGTAGTAGTTCACAAACAACTACTGTTCATAAAACAGATCTAGCTAAGATCTTTAAAGAAAATGAAACTACTAAATCAACAGATGTTTTTAAAGGTGAATATCAAAAAAAGTTCAGTAATGCTACTAGTACAAGTAACAGTGATAGTAGTAATAATTCTGCAATCGTTGATCTAAAAGAACTTAAAAAAGATAACAATAGTCAACCTGATTTAATCTTAGCACGTGGTAAAGATGGTATCCATCTAATGGGTGTTGATGGTGGTGGTTATTATCTCTCTGAATCAGGACGTGATGTAAATAAACAAAAACAATTCTTATTATTCCGTGCACTACAAACCAAATATGGCTTAATTGATACAAACACTACTTATGACTTTAAGTTGTTTGATGAAGTGAAAAAACACTTTGATAAGAACAGAGTTTTATTTTTATTTAATGCCCTTTTTAAATTAGTAGACAGCAAAGAAAGTAACTTTTTAAGTTTCCCTCAGTTTAAAAAGTTTAGTGATAGCATTATCACTGTTAAAAACGAGTTGAAGGATTTAGTTGAAAGTCAATATCAACAAATTGTTTTTAATGAAGTTGCTACTGCAGAAAACAAAGTTGCTTTAAAACTAGCAGAACGTAACCAACCTTTCATTGATAATGAGCGAAATAAACAAATATGGATGAACGGTTTAGCTGCTGTTTTACCTTATGAACAAGATAGTAAAACAGGACACTACAATGAACTTGGTATTTACTATAAAGACATCATTGATAAAGTTAGTTCAAATACAAGTAATAGTAATAGTAATAGTAATAGTAGTAGTAGTGATCCTTTTTCAAAAAAGATAATTGACAAGTTAAAAGAGAATAAAAAGAAAGTTGAAGCAGCAGTTAAAAAGCATGTTGATGAACTAAAAGTTTCGGTAATTCCCTCACCGCAATATTCACAAATTATCCTTGTTGATACCAAACTTTCATCTGATCCTAGAAACACTTCTCTAGCACTTAATTTAGCTTTAAATGCTGTTTTATCAAGTGATGAGTTACAAAATACCATTAGACGAGATTATTTTGTCAATGATGATCAGTTTAAACAAGCGATTGATTTAGATAAATTAACTTTTAAAAACTGA", "aa": "MLKASDMFDTFDPSFSAAFIQGYLALQKKSKGAEQTEYTKLEKDKSIIENFFVENNSAKAAMKSASSSSQTTTVHKTDLAKIFKENETTKSTDVFKGEYQKKFSNATSTSNSDSSNNSAIVDLKELKKDNNSQPDLILARGKDGIHLMGVDGGGYYLSESGRDVNKQKQFLLFRALQTKYGLIDTNTTYDFKLFDEVKKHFDKNRVLFLFNALFKLVDSKESNFLSFPQFKKFSDSIITVKNELKDLVESQYQQIVFNEVATAENKVALKLAERNQPFIDNERNKQIWMNGLAAVLPYEQDSKTGHYNELGIYYKDIIDKVSSNTSNSNSNSNSSSSDPFSKKIIDKLKENKKKVEAAVKKHVDELKVSVIPSPQYSQIILVDTKLSSDPRNTSLALNLALNAVLSSDELQNTIRRDYFVNDDQFKQAIDLDKLTFKN", "aa_hexdigest": "e7e00997f54a50ead8d402586c071917", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 49520.05660000003, "isoelectric_point": 8.498367500305175 }, "id": "KMADJIBOEG_717", "locus": "GCA000292445_00702" }, { "type": "cds", "sequence": "contig_1", "start": 385504, "stop": 385866, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGAATTGTTGCTGTTGCCTGTGCACAACCAAACTCCAGAACCATTGAAAATCTCTTTAGACCCAGTAGTGCTTTTACTGATAAAAATGATGGTAGTATTAATGCCACACTTTATAAAGCACTTGAAAATAGAGAAGGACTAACGCAATATTTAACGATGCGCCTAGCGCCAGTATTGCGCAATTTTTATGAAGAAAATGTTGATGATGACATTAAGAGAAATTTAAGAACTTTTAATACTGATACAGACAATAGTTTTGTTAATCAAGAACAAAACTTAAGAAATCAGTATCGGGGTGATTATTTAGTTAGGTTACAAACTGATATTTTAGATAACACTGGTGGAAATCAAGCCAACTGA", "aa": "MGIVAVACAQPNSRTIENLFRPSSAFTDKNDGSINATLYKALENREGLTQYLTMRLAPVLRNFYEENVDDDIKRNLRTFNTDTDNSFVNQEQNLRNQYRGDYLVRLQTDILDNTGGNQAN", "aa_hexdigest": "f6b597b7100c4a3218f8b2035cabfd0a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13696.93039999998, "isoelectric_point": 4.871010780334472 }, "id": "KMADJIBOEG_718", "locus": "GCA000292445_00703" }, { "type": "cds", "sequence": "contig_1", "start": 385980, "stop": 386195, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGACTTGAAATAGAAAACGGGCTTGAATTTGTCAATGATCCTGTGGTAAATGAACTTGGCAAGATCTGTTTTTTTCATCCTTTTACAGGTAATTTAACAAACAAACTTAGTTTCAGAAGTCATTTCAATAGATACAGTTTTTATGCCATTAACTACCCAGGGCATGGTAATAGTGTTATTAACAATCCAAAGCAACTTGAATTTAGTTACTGA", "aa": "MRLEIENGLEFVNDPVVNELGKICFFHPFTGNLTNKLSFRSHFNRYSFYAINYPGHGNSVINNPKQLEFSY", "aa_hexdigest": "0559d41656c46e83637a9a9b1e1bb444", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8287.231399999997, "isoelectric_point": 6.815188407897949 }, "id": "KMADJIBOEG_719", "locus": "GCA000292445_00704" }, { "type": "cds", "sequence": "contig_1", "start": 386196, "stop": 386609, "strand": "+", "frame": 3, "gene": "menH", "product": "Putative esterase/lipase 1", "db_xrefs": [ "COG:COG0596", "COG:HR", "EC:3.1.-.-", "GO:0016020", "GO:0016298", "SO:0001217", "UniRef:UniRef50_P75333", "UniRef:UniRef90_Q49412" ], "nt": "TTGGAAATTACAAAACAGTTTTTTGATAAACATAACTTAAAGGATGTAATCTTATTTGGTCATTCTATTGGCGGTGGTTTAGCAGTTGCTTTAACTAACTATTTAAGCAGTGATCAATATAAAGCAGTCTTGTTAGAAGCACCATTAAATCCTGCTATTGTTGAAACTCCTTTAAATATTGTTCAAAATCTCATTCCTGATCCTGATAGTGATTTTGCTGTTATTCAAAAGTGTCTTGTTTACAATATTGAGAAAAAACTTGGGGCAAACTTTAAAGAATATTGTGAAAGAGAAAAGCAAAAATCAATTCACCAAAACCAACGCCTTAAAGTGATGTTAGAACCTTCTACACTCAAACAAAACATAGTTTTAATCAATGCAGCTTTTTTAAAGTTAAATTGTCCTGCTTTGTGA", "aa": "MEITKQFFDKHNLKDVILFGHSIGGGLAVALTNYLSSDQYKAVLLEAPLNPAIVETPLNIVQNLIPDPDSDFAVIQKCLVYNIEKKLGANFKEYCEREKQKSIHQNQRLKVMLEPSTLKQNIVLINAAFLKLNCPAL", "aa_hexdigest": "117a70ad617aa154477b429457924bb0", "start_type": "TTG", "rbs_motif": null, "genes": [ "menH" ], "truncated": "both", "pseudogene": { "start": 385980, "stop": 386786, "observations": { "mutation": [ 386193, 386607 ], "stop": [ 386193, 386607 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49412", "query_cov": 1.0, "subject_cov": 0.5111940298507462, "identity": 0.993, "score": 266.0, "evalue": 2.03e-88, "gene_start": 1, "gene_end": 137, "reference_start": 73, "reference_end": 209, "reference_sequence": "MRLEIENGLEFVNDPVVNELGKICFFHPFTGNLTNKLSFRSHFNRYSFYAINYPGHGNSVINNPKQLEFSYWLEITKQFFDKHNLKDVILFGHSIGGGLAVALTNYLSSDQYKAVLLEAPLNPAIVETPLNIVQNLIPDPDSDFAVIQKCLVYNIEKKLGANFKEYCEREKQKSIHQNQRLKVMLEPSTLKQNIVLINAAFLKLNCPALWIHGKQDGIIKYLPSKAYYESLNNKQIQFKAIEAAAHTPYFEQPQKFLSLVNDFFQLIS" }, "paralog": false, "identity": 0.9888059701492538, "score": 535.0, "evalue": 1.13e-196, "description": "Internal stop codon at 386193, 386607. Nonsense mutation around 386193, 386607.", "psc": { "uniref90_id": "UniRef90_Q49412", "gene": "MenH", "product": "Putative esterase/lipase 1", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75333", "cog_id": "COG0596", "cog_category": "HR", "go_ids": [ "GO:0016020", "GO:0016298" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49412", "EC:3.1.-.-", "UniRef:UniRef50_P75333", "COG:COG0596", "COG:HR", "GO:0016020", "GO:0016298" ] }, "pscc": { "uniref50_id": "UniRef50_P75333", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75333" ], "product": "Putative esterase/lipase 1" } }, "id": "KMADJIBOEG_720", "locus": "GCA000292445_00705" }, { "type": "cds", "sequence": "contig_1", "start": 386814, "stop": 387410, "strand": "-", "frame": 2, "gene": "rpsD", "product": "30S ribosomal protein S4", "db_xrefs": [ "COG:COG0522", "COG:J", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0042274", "KEGG:K02986", "SO:0001217", "UniRef:UniRef50_P46775", "UniRef:UniRef90_P47553" ], "nt": "ATGAAATATACTGGTAGTATTTTCAAACGATCAAGACGTTTAGGTTTTTCTTTACTTGAAAACAACAAAGAGTTTTCCAGAGGAAAAAAACGTAAATCTATTCCCGGTCAACATGGAAATAGGTTTCGTTCCTCAACTTTATCAGGTTATGCCCAACAACTCCAAGAAAAACAAAGGATGCAATACATGTATGGTATTACTGATAAACAATTTCGTAGGTTATTTCGCTTTGTTTTAAAACAAAAAGGTAACTTAACAGTTAATTTATTTAGAGTTTTAGAATCACGTTTGGATAACATAGTTTACAGAATGGGTTTTGCACCAACAAGAAAGAGTGCAAGACAAATGGTAAACCACGGTCATGTGATTTTAAATGATCAAACTGTTGACACCCCTTCAATCATCATTAATCCCGGGGATAAAGTCCGTTTAAAAGCAAGAATAACTAAATCCCCATTAGTTAAAAATTTTATTGAAAACAGTGTTATCTCATCATTTGTGGAAACCAACAAAAAGGCATTTGAAGGTACTTATATAAGATTCCCAGAGCGTAGCGAACTACCTGCTGGAATAAATGAATCTTACGTTGTTGAGTGA", "aa": "MKYTGSIFKRSRRLGFSLLENNKEFSRGKKRKSIPGQHGNRFRSSTLSGYAQQLQEKQRMQYMYGITDKQFRRLFRFVLKQKGNLTVNLFRVLESRLDNIVYRMGFAPTRKSARQMVNHGHVILNDQTVDTPSIIINPGDKVRLKARITKSPLVKNFIENSVISSFVETNKKAFEGTYIRFPERSELPAGINESYVVE", "aa_hexdigest": "8b442689268c809deea20a5253337d9c", "start_type": "ATG", "rbs_motif": "ATTTA", "psc": { "uniref90_id": "UniRef90_P47553", "query_cov": 1.0, "subject_cov": 0.9658536585365853, "identity": 0.995, "score": 383.0, "evalue": 1.98e-134, "valid": true, "gene": "rpsD", "product": "30S ribosomal protein S4", "uniref50_id": "UniRef50_P46775", "cog_id": "COG0522", "cog_category": "J", "kegg_orthology_id": "K02986", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0042274" ] }, "pscc": { "uniref50_id": "UniRef50_P46775", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P46775" ], "product": "Small ribosomal subunit protein uS4" }, "genes": [ "rpsD" ], "id": "KMADJIBOEG_721", "locus": "GCA000292445_00706" }, { "type": "cds", "sequence": "contig_1", "start": 387433, "stop": 389568, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCATTATGAAAGTAAATCTGAACCAATTCATGACTATAAATTTGGGAGTGATTTATCTCAATCAAACAGCAATAATTCGTTAGAGTCTGAACCTGTTAAGTTTAATAGTGAAACTGCACCTGATGCACACTTTGAAAGTCAATCTGAACCAGTTGATCAAGTTCAATACGACATTTATCAAAATGAAGAGCTAAAACCAACTCTTGACCAGCCTTCTTCAGATGATTATTTTGCAAAACAACCAACTGATGAAAACTATGGTTTTGATAACGACCTACCACCAGAAGTAAAACAACCTGAATCAGTTGTTGACCAGCCTTCTTCAGATGATCATTTTGCAAAACAACCTGAATCAACAACTGATAGCTACAGCTTTGACAGTGATTTACCTCAACCAACCCTTGACCAACCTTCTTTAGATGATCATGTTCAGTACAACTTTGATCACCATGAAGAGCTCAAACCAGTTGCTGAAGAACAAAATAATTATCAAGTTGGATTTGATCAAGTTCAAGCTAATCTTGATAATAATGAGGAAATACAACCAACTGCTGAAAAAAAAGTAACTACTGATTTTGAAAGTAAACAAGCCCAAGTAGTTGATAGTTACCAATTACCAATTGATACAGATCAACAAGACCAAACAACTTTTTCTTCTTCATTTGAAACTCAACCAACAGTTGAGCAGTTTGATCAAGTAAATAGCGAAGTTAACGATCAATTCAAACCTGAAATAACTAAGGAGCCAGTTTTAGAAAGTAGTTTCAATAAACAAGATGTAGTTGAAACTAGTAACTACACTAATAATTTACAGAAATTTGATATTCAGTCTGATAATAAGATAACTATCACTACCAAAAAAAGTTCACCCCAAATCCCTACAACTTTACCTATTAGTTTTGTATCCAACCGTATTGAATATAAACCAGTTGAAACTTTAGCTTTAGATAATAAAGAAAGTCAGCAAGAACAAATTACCATTAATTCCATAACTGAAGATTCCAAAACACTTGCAAAAACATTATCTGTCCAATTGCAACAAATTAATAGCTTAAATAATCAATCAATTGTTACTAGTGAATCAGTAAGACTCGATAAAAAAGATGATCAACTAACTATCAATACTGTTAATAGCGAAGACCAACAACCTAAAATTGAAGTTTTTGTCAAAGCAAAAGAACCTGTTGAAGAACACTCAATTACTCAAAATAAGCAATCGGTTGAAGATAAAAGTGAACTTGATAATTTCAATAAAAAATCAGATCTTTACAAGATTATTAGTGAATTGAAACGTGGTGAATTAAACCCTACAATTAACTTTGATGCAATCTTTCAAATGAATGATTATCAGATGTCTGTTAAACAATCATTTATCCATCTCAATGATTTTGTTACCAACTATAAGAACCAAATTAGTGAACGTTACTTAATAATTAAAAAGGAATTACAAAGTGAACTTTCCCGTTTAATTGATCAAAATGAGAACCTTAACGTTCAATTTAATAACGCTAAGAATCTAACAACACTTCAAAAAGAAGAAATGATCAGATCATTAGCTAGTGATTTTGCTATTGCTTACAAACCAAGTAATAGTTATGAACAACTGCAAAAATCTGGAGAAATTATGCGTCATGTTCAACGTGCTATCACTGAAAATGAAAAGAAGATAGAAAGCATTCAAGGTAGTTTAAAACAACTTAAAACTGTTTATAACTCTTGTTGTGAAACTATTATGAACAACATTAATAAGCTTGATAATACGCTCCGCTTTGCAAAGAAAGAAAAAGATCCTTTACTTTTAAGCAATTTTGATAGTGTAACAGATAATGGGTTAGTAGAACCTAATCAGTTAATGGATGATTTAATAGATTTTTCCAACACTTTTGATAACATCTCCAATGAACAGTTAGATGATTTTATCTATGAAAACATGGATCGTAACATCGATTTTGAATTTGAAGGTTTTAACAATGATTTTGTTGACATTGATGCCAAAGTAATGGATTCAATGAGTACTTTTAGTGTTAATGATTTGGATATAGAAACACTAGTTCCTGATCGTACTTCCAATTTCAGTTCATTATTAGATGAAGACTTATTTGAAAGCAGTGGTGATTTTAGCCTTGATTATTAA", "aa": "MHYESKSEPIHDYKFGSDLSQSNSNNSLESEPVKFNSETAPDAHFESQSEPVDQVQYDIYQNEELKPTLDQPSSDDYFAKQPTDENYGFDNDLPPEVKQPESVVDQPSSDDHFAKQPESTTDSYSFDSDLPQPTLDQPSLDDHVQYNFDHHEELKPVAEEQNNYQVGFDQVQANLDNNEEIQPTAEKKVTTDFESKQAQVVDSYQLPIDTDQQDQTTFSSSFETQPTVEQFDQVNSEVNDQFKPEITKEPVLESSFNKQDVVETSNYTNNLQKFDIQSDNKITITTKKSSPQIPTTLPISFVSNRIEYKPVETLALDNKESQQEQITINSITEDSKTLAKTLSVQLQQINSLNNQSIVTSESVRLDKKDDQLTINTVNSEDQQPKIEVFVKAKEPVEEHSITQNKQSVEDKSELDNFNKKSDLYKIISELKRGELNPTINFDAIFQMNDYQMSVKQSFIHLNDFVTNYKNQISERYLIIKKELQSELSRLIDQNENLNVQFNNAKNLTTLQKEEMIRSLASDFAIAYKPSNSYEQLQKSGEIMRHVQRAITENEKKIESIQGSLKQLKTVYNSCCETIMNNINKLDNTLRFAKKEKDPLLLSNFDSVTDNGLVEPNQLMDDLIDFSNTFDNISNEQLDDFIYENMDRNIDFEFEGFNNDFVDIDAKVMDSMSTFSVNDLDIETLVPDRTSNFSSLLDEDLFESSGDFSLDY", "aa_hexdigest": "e13978e5dcd8cedc3cc31fd8dc42f63c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 81673.16070000037, "isoelectric_point": 4.294720268249511 }, "id": "KMADJIBOEG_722", "locus": "GCA000292445_00707" }, { "type": "cds", "sequence": "contig_1", "start": 390081, "stop": 390476, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTGAGGTAAATCACTGTCAAAGCTGTAGCTATCAGTTGTTGATTCAGGTTGTTTTGCAAAATGATCATCTGAAGAAGGTTGGTCAACAACTGATTCAGGTTGTTTTACTTCTGGTGGTAGGTCGTTATCAAAACCATAGTTTTCATCAGTTGGTTGTTTTGCAAAATAATCATCTGAAGAAGGCTGGTCAACAACTGATTCAGGTTGTTTTACTTCTGGTGGTAGGTCGTTATCAAAACCATAGTTATCTTCAACTGATTCAGGTTGTTTTACTTCTGGTGGTAAATCGTTATCAAAACCATAGTTATCTTCAACTGATTCAGGTTGTTTTACTTCTGGTGGTAAATCACTGTCAAAACCATAGTTTTCATCAGTTGGTTGAGATTCTTTAG", "aa": "MVEVNHCQSCSYQLLIQVVLQNDHLKKVGQQLIQVVLLLVVGRYQNHSFHQLVVLQNNHLKKAGQQLIQVVLLLVVGRYQNHSYLQLIQVVLLLVVNRYQNHSYLQLIQVVLLLVVNHCQNHSFHQLVEIL", "aa_hexdigest": "628564ab6aa71480e9a05bc876c5b9b7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15224.8124, "isoelectric_point": 9.153624153137208 }, "id": "KMADJIBOEG_723", "locus": "GCA000292445_00708" }, { "type": "cds", "sequence": "contig_1", "start": 390511, "stop": 390852, "strand": "-", "frame": 1, "gene": null, "product": "EAGR box-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B418BE", "UniRef:UniRef90_UPI0001B418BE" ], "nt": "ATGGCTAAAAACAAGCAATCGGTTTTTGAAGAAAAAAACTATACCCAAACCGAACCTGAAAACATTTTTGGTGATCTTTATGATGGTAAATCCACTGTTGAAGAAGACTCTAATATTAAGGTAGCATATGATGCTGATGGTAATGGCTATTATATAGCTTTTAACAAAGAAACAGGGGTTTACTATGATCCTTATGGAGATACTGAATATGATATCTCCCAACTCTTTGATGAAAATGGGAATCCGTTTGTTTTTGATGAAAAGCAAGAAGAGAATGATTATTTAAAGTATGTTGGTAACCCTGACTATGGTTCTTATGATGAAAATGGTGAGTGGGTTTGA", "aa": "MAKNKQSVFEEKNYTQTEPENIFGDLYDGKSTVEEDSNIKVAYDADGNGYYIAFNKETGVYYDPYGDTEYDISQLFDENGNPFVFDEKQEENDYLKYVGNPDYGSYDENGEWV", "aa_hexdigest": "f399256b69cada5ca6c2cacfa4d20a6c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 390412, "stop": 390852, "observations": { "mutation": [ 390513 ], "stop": [ 390513 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001B418BE", "query_cov": 1.0, "subject_cov": 0.6420454545454546, "identity": 0.991, "score": 231.0, "evalue": 3.6e-76, "gene_start": 1, "gene_end": 113, "reference_start": 1, "reference_end": 113, "reference_sequence": "MAKNKQSVFEEKNYTQTEPENIFGDLYDGKSTVEEDPNIKVAYDADGNGYYIAFNKETGVYYDPYGDTEYDISQLFDENGNPFVFDEKQEENDYLKYVGNPDYGSYDENGEWVWSGYFENDQWISTKESQPTDENYGFDSDLPPEVKQPESVEDNYGFDNDLPPEVKQPESVVDNY" }, "paralog": false, "identity": 0.9863013698630136, "score": 309.0, "evalue": 1.83e-109, "description": "Internal stop codon at 390513. Nonsense mutation around 390513.", "psc": { "uniref90_id": "UniRef90_UPI0001B418BE", "product": "EAGR box-containing protein", "uniref50_id": "UniRef50_UPI0001B418BE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001B418BE", "UniRef:UniRef50_UPI0001B418BE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001B418BE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B418BE" ], "product": "EAGR box-containing protein" } }, "id": "KMADJIBOEG_724", "locus": "GCA000292445_00709" }, { "type": "cds", "sequence": "contig_1", "start": 390912, "stop": 391625, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MG313", "db_xrefs": [ "GO:0022857", "SO:0001217", "UniRef:UniRef50_Q50364", "UniRef:UniRef90_Q49414" ], "nt": "TTGTTCGGATGGATCTTTGGTCCTATTTGGGGTTTTATTTTTGGATTTTTATCTGATACTATTGATTGGTTAACAAAGGGTTATGTGTGGTTTTGGATGTTTGCATTGCAAAAGCCACTCTTTGCTTTTCTTGCTGGCATAGTTAAAGGGATTTATCTAGTTAGAAAAAATGCCAGTAATTACAAGGTTGATTTTTGGGTGTTACAAACACTATTAGTAACTTTTTTTGTTGTTAGTGTAACACTGCTTTTACTTTATCTAACTAATAATGAGTTTCAGCAAATTGGAACAAAAAATTTTCAAACTGCTGATCTTAGTGTTAATGTTGTTGTTTTACAAATCATAACTTTAGTTAGCTTTGTTATTTTCTTTTTAGTAACAGAAGGATTTTTAGGGTTTGTTTATGTAAAAAAGAGAAATAAAGAACAAGCACTTTTAACTATTTATAGCTTGGTTCTAATGGTTTTAATGTCAGTAGTTGTTTCTATCCTTTTGGGGACAATTGCTGCCATTGAGTTTATTACTTTTATTAATAATGGCAGACCAAGTAATAACTTTGTTTTGTATGGGGTTTATTTCTTTCTTTTACCAAGAGTATTGGTGCAAGCGTTATTATTACCTATCTATGTAGCTTTGTTTTACCCTTTAATAGGGATTGTGGAAAATAATTTAAAAAATTATTTATTACTATTTACGCTTTCATGGAAGAGTTAG", "aa": "MFGWIFGPIWGFIFGFLSDTIDWLTKGYVWFWMFALQKPLFAFLAGIVKGIYLVRKNASNYKVDFWVLQTLLVTFFVVSVTLLLLYLTNNEFQQIGTKNFQTADLSVNVVVLQIITLVSFVIFFLVTEGFLGFVYVKKRNKEQALLTIYSLVLMVLMSVVVSILLGTIAAIEFITFINNGRPSNNFVLYGVYFFLLPRVLVQALLLPIYVALFYPLIGIVENNLKNYLLLFTLSWKS", "aa_hexdigest": "42a2eb2c5f01879fe6485caf3da6f1cb", "start_type": "TTG", "rbs_motif": "TTCTA", "psc": { "uniref90_id": "UniRef90_Q49414", "query_cov": 1.0, "subject_cov": 0.8257839721254355, "identity": 0.97, "score": 423.0, "evalue": 2.55e-148, "valid": true, "product": "Uncharacterized protein MG313", "uniref50_id": "UniRef50_Q50364", "go_ids": [ "GO:0022857" ] }, "pscc": { "uniref50_id": "UniRef50_Q50364", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50364" ], "product": "Uncharacterized protein MG313 homolog" }, "genes": [], "id": "KMADJIBOEG_725", "locus": "GCA000292445_00710" }, { "type": "cds", "sequence": "contig_1", "start": 391651, "stop": 391764, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAATACATTAGTAACACTAATTGAAAAGATAGAAAAGATAAAGGTTAGTGCTAATAAAACACTAGCTCAAACTAACAGTTGTAAACTCTTTAAAAGTTTCAACGGATAG", "aa": "MLNTLVTLIEKIEKIKVSANKTLAQTNSCKLFKSFNG", "aa_hexdigest": "e162a67b8f6d3380cc94e925f57afdf4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4126.8806, "isoelectric_point": 9.748022651672361 }, "id": "KMADJIBOEG_726", "locus": "GCA000292445_00711" }, { "type": "cds", "sequence": "contig_1", "start": 391820, "stop": 392008, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGTTTTTAGCATTGTTCCTTTTAGCATCATTAGTGTAATTGCTTTTTCATTAAGTCCTAAATTAAACTTAGAACAAACTAATGAGATATTTAATAAGTACTTTCAAGAGGAATTGGCAAAACCAATTACTAGTGATGAAAGTAAAACTACTTTAGATATCCCCCCACCTACTATTTTTGGTTAA", "aa": "MFVFSIVPFSIISVIAFSLSPKLNLEQTNEIFNKYFQEELAKPITSDESKTTLDIPPPTIFG", "aa_hexdigest": "d7a15b400e85a2dcbb20be8117125756", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6995.002, "isoelectric_point": 4.4943956375122065 }, "id": "KMADJIBOEG_727", "locus": "GCA000292445_00712" }, { "type": "cds", "sequence": "contig_1", "start": 392182, "stop": 392532, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGCCATCTTTTTCATTTCGATAGCATACGTTGTGATGGTCAATGTCTTTTTTACCCACTTTGTTGATAAGAACAGTCCACTTTTCAATGTTAATAATGACCAAAATACTGTTCAGCAAAGTAATGCAGATACAACAAATCTAAACCGCTTTTTTGCTTTAAGTTCATCTAACTTGATTACCATGATCTTCCTGGCTTTTGGTGGTGCTTCTTTTTCTTTGCTTTTCAGGGTAAACCCTATAGCTTTGCAACTAGAGGTCAAACCAATAGAGCAATGCGGTATTTACGTAGTGAATTTGGGGTCAAAGAGTTTCCCAAAATTAATGATAACTACCGTTATAAGGTAA", "aa": "MIAIFFISIAYVVMVNVFFTHFVDKNSPLFNVNNDQNTVQQSNADTTNLNRFFALSSSNLITMIFLAFGGASFSLLFRVNPIALQLEVKPIEQCGIYVVNLGSKSFPKLMITTVIR", "aa_hexdigest": "89ecaf6062008aff186310a59cbdca11", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12989.051799999988, "isoelectric_point": 8.978270149230958 }, "id": "KMADJIBOEG_728", "locus": "GCA000292445_00713" }, { "type": "cds", "sequence": "contig_1", "start": 392557, "stop": 392829, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAATAAAGAGTTGAATAGCTTTACCAAACTTGCTGAAACTCAAGTTAAACCCGTTAGTGAGTTACAAGAAAACAATAAAACAGTTAAAGATAACCCAAGTTTTCAAACTATAAACAATTCTCAACAGCTAAACTCAGTACTTGAGAATAATATTTTGCAACAAACCCTCCAAGTTCGTGCCAGAGATCGTGCCTTTGGTAGGTTTACCTCTGAAAAACTAGTTGGTAAGATCTTTGAGTTTCAGTTTAAGAGTCAGTGGATTAAATGA", "aa": "MFNKELNSFTKLAETQVKPVSELQENNKTVKDNPSFQTINNSQQLNSVLENNILQQTLQVRARDRAFGRFTSEKLVGKIFEFQFKSQWIK", "aa_hexdigest": "9fe2be7279939eb254ccfa5df0d6eab4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10537.804299999994, "isoelectric_point": 9.695158576965333 }, "id": "KMADJIBOEG_729", "locus": "GCA000292445_00714" }, { "type": "cds", "sequence": "contig_1", "start": 392928, "stop": 393149, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACGTTTAGTGAAATTCTAACTAAGGTACAAAATAATCTTGACATTGTTTTTAATAACGAAACTTTAAGAACAAGGATAAAAACTGATAGTGATTTTGCCAAAACAATTTTGGCACAACTCAAATTACTTTATTTTTTAGAGGAAAAACAAAAAAGAGTTAAAACAAAAAACCAGACCACTTTCTTTTTGGTAGTTTTCATGATAAGTTTATCCAATTAG", "aa": "MTFSEILTKVQNNLDIVFNNETLRTRIKTDSDFAKTILAQLKLLYFLEEKQKRVKTKNQTTFFLVVFMISLSN", "aa_hexdigest": "c9faa3f977422652ba523345d74042ae", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8620.002299999998, "isoelectric_point": 9.69619007110596 }, "id": "KMADJIBOEG_730", "locus": "GCA000292445_00715" }, { "type": "cds", "sequence": "contig_1", "start": 393173, "stop": 393637, "strand": "-", "frame": 3, "gene": "holA", "product": "DNA polymerase III subunit delta", "db_xrefs": [ "COG:COG1466", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0006261", "GO:0009360", "SO:0001217", "UniRef:UniRef50_Q49416", "UniRef:UniRef90_Q49416" ], "nt": "ATGGGGGTAATTTACCAAGAAATTAACAAACTTAGCTTGTTAGGTAAAAATGAAATCAAAGATAACAAATTAGTTGAAACAGTTATTTGTGATTATCAACCTGTTCAAATTTACAAATTAACCAAAGCTCTAACAAATTCACAAATTGTAAAGGCATTTAAATATATTGATGAATTGGCAAGTACAAAACCAAATTTTGCCACCCAATTTTTAGAATTTTTTAGTGGGGAGTTGTTATTAGCATTGATGGTTAAAAGTTGTAATCCAAAGCAACTAACTAACATTAATCTAAATGTTAATCAGTTTCGCTTAATTGCTATTCAAAGTCAGTACCATAACTTTACTAGCAAAGTTTTAGTAAATATAATTAATGCAATTCAAAAATTGGATATTAAATTAAAGCACAATGATGGCTTTGCCATTCCGTTGTTAAAAAACTTTGCTTTAAGCTTTTTTACTAACTAA", "aa": "MGVIYQEINKLSLLGKNEIKDNKLVETVICDYQPVQIYKLTKALTNSQIVKAFKYIDELASTKPNFATQFLEFFSGELLLALMVKSCNPKQLTNINLNVNQFRLIAIQSQYHNFTSKVLVNIINAIQKLDIKLKHNDGFAIPLLKNFALSFFTN", "aa_hexdigest": "3684d242b37a7e23703ab1ee9e3b5517", "start_type": "ATG", "rbs_motif": null, "genes": [ "holA" ], "truncated": "3-prime", "pseudogene": { "start": 393173, "stop": 393937, "observations": { "mutation": [ 393664, 393739 ], "stop": [ 393664, 393739 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49416", "query_cov": 1.0, "subject_cov": 0.5185185185185185, "identity": 1.0, "score": 305.0, "evalue": 9.4e-103, "gene_start": 1, "gene_end": 154, "reference_start": 144, "reference_end": 297, "reference_sequence": "MTIIYGQDIGLIHQKLSQIKSNSPYKTIWFKDLKQLYDLFSQPLFGSNNEKFIVNNCSFLEKTSLTRQENLCLEKLKTTDVVLTVYTDNPFSGIKTIKSITTVFCDKLDWKSMHKAIGEVCRELNLKLDLEIIDWLANALPLNMGVIYQEINKLSLLGKNEIKDNKLVETVICDYQPVQIYKLTKALTNSQIVKAFKYIDELASTKPNFATQFLEFFSGELLLALMVKSCNPKQLTNINLNVNQFRLIAIQSQYHNFTSKVLVNIINAIQKLDIKLKHNDGFAIPLLKNFALSFFTN" }, "paralog": false, "identity": 0.9921259842519685, "score": 493.0, "evalue": 1.78e-179, "description": "Internal stop codon at 393664, 393739. Nonsense mutation around 393664, 393739.", "psc": { "uniref90_id": "UniRef90_Q49416", "gene": "holA", "product": "DNA polymerase III subunit delta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_Q49416", "cog_id": "COG1466", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0006260", "GO:0006261", "GO:0009360" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49416", "EC:2.7.7.7", "UniRef:UniRef50_Q49416", "COG:COG1466", "COG:L", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0006261", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_Q49416", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49416" ], "product": "Probable DNA polymerase III subunit delta" } }, "id": "KMADJIBOEG_731", "locus": "GCA000292445_00716" }, { "type": "cds", "sequence": "contig_1", "start": 393737, "stop": 394066, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAATAATTTATGGTCAAGATATTGGGCTAATTCACCAAAAATTAAGTCAAATCGAAAGCAATTCTCCATATAAAACTATTTGGTTTAAAGATCTTAAGCAACTTTATGATCTCTTTTCACAACCATTATTTGGTTCTAATAATGAAAAGTTTATTGTCAATAATTGCAGTTTTTTGGAAAAAACAAGCTTAACTAGACAAGAAAACTTGTGTTTAGAAAAACTAAAAACAACTGATGTAGTTTTAACTGTTTATACTGACAATCCTTTTAGTGGGATTAAAACCATTAAATCAATCACAACTGTTTTTTGTGATAAATTGGATTGA", "aa": "MTIIYGQDIGLIHQKLSQIESNSPYKTIWFKDLKQLYDLFSQPLFGSNNEKFIVNNCSFLEKTSLTRQENLCLEKLKTTDVVLTVYTDNPFSGIKTIKSITTVFCDKLD", "aa_hexdigest": "418818bf720212599900b0c7908f78f8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12563.323999999979, "isoelectric_point": 6.555491065979004 }, "id": "KMADJIBOEG_732", "locus": "GCA000292445_00717" }, { "type": "cds", "sequence": "contig_1", "start": 394254, "stop": 394625, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTACCTGTTTTTAGTAGGATTTGCTTTTTCAGCACTAAGAGTTTTTATCTCAACACTGTTAAAACAGGTTTTTAAAAAACAGTTACCAGAAGATAACTTAAGTTTAACTGCACTTTTAATCATTCTTATTAGTAACCATGCTTTAAATAACTTTGGTTTTAATTTTTCTTTTCTAGCTTGTTTTGTATTGTTATTTGTCAACAAACTAAAGTTATTAAAAGCTTTAAAACCGCTTGTCAGTTCTAGTTTAATCTTGATTGTTATCAGCCCCTTAAGCTTGTATTTAAATTCAAGACTAAATGCTTTTAGTGTCTTGTTTAACCTTTTGTTTAGCCCAATTGCCTTATTTTATTTTTGTGTTAGTTGA", "aa": "MIYLFLVGFAFSALRVFISTLLKQVFKKQLPEDNLSLTALLIILISNHALNNFGFNFSFLACFVLLFVNKLKLLKALKPLVSSSLILIVISPLSLYLNSRLNAFSVLFNLLFSPIALFYFCVS", "aa_hexdigest": "9a7bc2cd2c7f55cedc503890d5f89855", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13871.699499999977, "isoelectric_point": 9.864645957946777 }, "id": "KMADJIBOEG_733", "locus": "GCA000292445_00718" }, { "type": "cds", "sequence": "contig_1", "start": 395286, "stop": 395756, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAACTTCAGGTTTACAAATTAAAGTTGTACCAAGATCAGCAGCTTTACTTCAATCAAAATTAGACACTGGTTTTCAACCTAGACAAGTTGAACGCACAACTGATTCAGATATCACTGTTAGTGTCTCTTCCCACGCTTCTCTTCTAGAGAAAATTAATGCTTTAAACCACCAAAGGATAATGAGTGATATTGCTCTAAAGAGTGATAACACTATAAAAAGTAGCAATTTCAGCCGTTTTTATCCTGAAAATGAGTATGTTGCAACTAAATACAGTGATCCACTTTACAGCGATACCAATCAAAGTTTAACTAGTGATCGTTTCTCTCTTGATTTTGACTACACACCAAAATCTAGAGTCAACAATTACACACCATTAAGATCAACTAATTTCCAAAATAATGCTATTTCTAACTATCGTTTTAGCAGAACACCAAGTAGTTATTATCCCTTAACAAGAAGACCATGA", "aa": "MPTSGLQIKVVPRSAALLQSKLDTGFQPRQVERTTDSDITVSVSSHASLLEKINALNHQRIMSDIALKSDNTIKSSNFSRFYPENEYVATKYSDPLYSDTNQSLTSDRFSLDFDYTPKSRVNNYTPLRSTNFQNNAISNYRFSRTPSSYYPLTRRP", "aa_hexdigest": "51c7cc00049032a9ae60be18c858b9dc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17803.540099999987, "isoelectric_point": 9.51767711639404 }, "id": "KMADJIBOEG_734", "locus": "GCA000292445_00719" }, { "type": "cds", "sequence": "contig_1", "start": 395796, "stop": 396938, "strand": "-", "frame": 2, "gene": "hmw3", "product": "Cytadherence high molecular weight protein 3", "db_xrefs": [ "GO:0005886", "GO:0020035", "GO:0033111", "GO:0042995", "SO:0001217", "UniRef:UniRef50_Q57081", "UniRef:UniRef90_Q57081" ], "nt": "ATGAACGATAAACAGAAAGCTAAAATAAACAAAGCCTATAACAAGCTTTTAAAAAAGATTAACAAAAGATATCCTGATGTTAGTGTTGTTTATGCCCGTGATCATAAAAATAAGGTTCATGCTCTATACCAAGATCCTGAATCAGGTAATATCTTCTCTTTAGAAAAAAGAAAGCAGTTAGCTAGTAACTATCCTTTGTTTGAATTAACTTCAGATAACCCTATTAGCTTTACAAATAACATTGTTAGCTTAAATGCTTATGATGATAAGAACAACTTGGTAACTGTTCAATATGATCAGGATAACAACACTTTCTATGACCAAAATGGTAATGTTTTGGATGTTTCTAGCTATACTGATGAGAAAAAGGTCCCTTTAATTAACTATTTAAGCAGTACTCAAACTTCTCAAGAACAACCAACCCAACAAGATTATCCTAGTATTGATGCAGGTCTTCCTAAGATAGAAGTTGATGACCAACCAAAAGCAGCTCAACACACTACTCTAGAAACTGAAAGTGAACCTGATGTCTTTGAATTAAATGACAGTTTAAATCAACCCCAACAACCTACAGAAAACTTGGGTGATGACCAGTTTGTTGAAAAAGAAGTGCCACCTACTCAACAGCTCCATCAGGATTTAGTCCACCAACAACCCGTTCAAGTTGATAGTGGATCTCAAAACCATAGTTTTAATAATTCCCCTTCTTTAAAACCACCACTAGTTAATAAACCAGCAAAGTTAGTTCAACCTGAAGTAAAACATATTCCTCAAGTTGAAGTTCAACCGAAACCTCAAATAGTTGAACCCAAAATTGAACCAAAACCAGAAGTGAAACATGTATCACATGTTGAAATTCAACCAAAACCAGAAGTGAAACCTGTTGTTGATTCAGTACCAGAAGTTAAACAACCTGAAGTAAAACATGTACCACATGTTGAAGTTCAACCAAAACCTGTTATTGATTTAAAACCTCAAAGGATTGAACCAAGAATTGAATCAAAACCAGAAGTAATAAAACATATTCCTCAAGTTGAAGTTCAACCGAAAGCTCAAATGGTTGAACCAAGAATTGAACCAAAACCTGAAACAAAATACATCCCTCAAGTAGAATCAACTCCTCAAGTTGAAGTTCACCACTGA", "aa": "MNDKQKAKINKAYNKLLKKINKRYPDVSVVYARDHKNKVHALYQDPESGNIFSLEKRKQLASNYPLFELTSDNPISFTNNIVSLNAYDDKNNLVTVQYDQDNNTFYDQNGNVLDVSSYTDEKKVPLINYLSSTQTSQEQPTQQDYPSIDAGLPKIEVDDQPKAAQHTTLETESEPDVFELNDSLNQPQQPTENLGDDQFVEKEVPPTQQLHQDLVHQQPVQVDSGSQNHSFNNSPSLKPPLVNKPAKLVQPEVKHIPQVEVQPKPQIVEPKIEPKPEVKHVSHVEIQPKPEVKPVVDSVPEVKQPEVKHVPHVEVQPKPVIDLKPQRIEPRIESKPEVIKHIPQVEVQPKAQMVEPRIEPKPETKYIPQVESTPQVEVHH", "aa_hexdigest": "396eb2ccd7399131adc2655bc880b92b", "start_type": "ATG", "rbs_motif": null, "genes": [ "hmw3" ], "truncated": "5-prime", "pseudogene": { "start": 395493, "stop": 396938, "observations": { "mutation": [ 395798 ], "stop": [ 395798 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q57081", "query_cov": 1.0, "subject_cov": 0.6343906510851419, "identity": 0.987, "score": 610.0, "evalue": 7.64e-215, "gene_start": 1, "gene_end": 380, "reference_start": 1, "reference_end": 380, "reference_sequence": "MNDKQKAKINKAYNKLLKKINKRYPDVSVVYARDHKNKVHALYQDPESGNIFSLEKRKQLASNYPLFELTSDNPISFTNNIVSLNAYDDKNNLVTVQYDQDNNTFYDQNGNVLDVSSYTDEKKVPLINYLSSTQTSQEQPTQQDYPSIDAGLPKIEVDDQPKAAQHTTLETESEPDVFELNDSLNQPQQPTENLGDDQFVEKEVPPTQQLHQDLVHQQPVQVDSGSQNHSFNNSPSLKPPLVNKPAKLVQPEVKHIPQVEVQPKPQIVEPKIEPKPEVKHVSHVEIQPKPEVKPVVDSVPEVKQPEVKHVPHVEVQPKPVVDLKPQRIEPRIESKPEVIKHIPQVEVQPKAQMVEPRIEPKPETKYIPQVESTPQVEVHHWKPEVKTEYQPQQPLPTSGLQIKVVPRSAALLQSKLDTGFQPRQVERTTDSDITVSVSSHASLLEKINALNHQRIMSDIALKSDNTIKSSNFSRFYPENEYVATKYSDPLYSDTNQSLTSDRFSLDFDYTPKSRVNNYTPLRSTNFQNNAISNYRFSRTPSSYYPLTRRPWRLTNISSYRSSFHSPTRLSSFRRTSLPFSSSYDGLRRYPSRSYWSKDF" }, "paralog": false, "identity": 0.9937629937629938, "score": 834.0, "evalue": 1.62e-305, "description": "Internal stop codon at 395798. Nonsense mutation around 395798.", "psc": { "uniref90_id": "UniRef90_Q57081", "gene": "hmw3", "product": "Cytadherence high molecular weight protein 3", "uniref50_id": "UniRef50_Q57081", "go_ids": [ "GO:0033111", "GO:0042995", "GO:0005886", "GO:0020035" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q57081", "UniRef:UniRef50_Q57081", "GO:0033111", "GO:0042995", "GO:0005886", "GO:0020035" ] }, "pscc": { "uniref50_id": "UniRef50_Q57081", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q57081" ], "product": "Cytadherence high molecular weight protein 3" } }, "id": "KMADJIBOEG_735", "locus": "GCA000292445_00720" }, { "type": "cds", "sequence": "contig_1", "start": 396953, "stop": 397297, "strand": "-", "frame": 3, "gene": null, "product": "P32 adhesin", "db_xrefs": [ "GO:0005886", "GO:0020035", "GO:0033111", "GO:0042995", "SO:0001217", "UniRef:UniRef50_Q49417", "UniRef:UniRef90_Q49417" ], "nt": "ATGCAACAACGCCCTGGTTTTAACCAACCAAACCAACAGTTCCAACCACACAATAATTTCAACCCAAGGATGAACCCAAACATGCAGCGCCCTGGGTTTAATCCAAACATGCAACAACGCCCTGGTTTTAACCAACCAAACCAACAGTTCCAACCACACAATAATTTCAACCCAACGATGAACCCAAACATGCAACGCCCTGGTTTTAACCAACCCCATCCTAACCAATTTGCACAACCAAATAACTTTAATCCCCAAATGCAACAACGCCCTGGGTTTAATCCAAATATGCAACGACGTTCTAACCCATCACAATTGATGCCAAAAGGCGGTTTAAAACCCTAA", "aa": "MQQRPGFNQPNQQFQPHNNFNPRMNPNMQRPGFNPNMQQRPGFNQPNQQFQPHNNFNPTMNPNMQRPGFNQPHPNQFAQPNNFNPQMQQRPGFNPNMQRRSNPSQLMPKGGLKP", "aa_hexdigest": "8e00ddfd6ba8db6e4620b8410194e294", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 396953, "stop": 397597, "observations": { "mutation": [ 397594 ], "stop": [ 397594 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49417", "query_cov": 1.0, "subject_cov": 0.40714285714285714, "identity": 0.877, "score": 101.0, "evalue": 5.51e-24, "gene_start": 1, "gene_end": 114, "reference_start": 167, "reference_end": 280, "reference_sequence": "MELNGFLRYKKLFIVLALLFTTILIVSLSLLAFALVVKTNGSELGVVFHQTEDNTTVIQGRSIVEQPWFIPTVAGSFGFSALAIILGLAIGLPIVKRKEKRLLEEKERQEQIAEQLQRISDQQEQQTVEIDPQQSQAQPSQPQVQQPLQPQFQQRVPLLRPAFNPNMQQRPGFNQPNQQFQPHNNFNPRMNPNMQRPGFNPNMQQRPGFNQPNQQFQPHNNFNPRMNPNMQRPGFNQPHPNQFAQPNNFNPNMQQRPGFNPNMQQRPNPSQLMPKGGLKP" }, "paralog": false, "identity": 0.9626168224299065, "score": 226.0, "evalue": 4.36e-75, "description": "Internal stop codon at 397594. Nonsense mutation around 397594.", "psc": { "uniref90_id": "UniRef90_Q49417", "product": "P32 adhesin", "uniref50_id": "UniRef50_Q49417", "go_ids": [ "GO:0033111", "GO:0042995", "GO:0005886", "GO:0020035" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49417", "UniRef:UniRef50_Q49417", "GO:0033111", "GO:0042995", "GO:0005886", "GO:0020035" ] }, "pscc": { "uniref50_id": "UniRef50_Q49417", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49417" ], "product": "P32 adhesin" } }, "id": "KMADJIBOEG_736", "locus": "GCA000292445_00721" }, { "type": "cds", "sequence": "contig_1", "start": 397309, "stop": 397518, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGGTCTTAAAAGCGGAACACGCTGTTGAAACTGTGGTTGAAGGGGTTGTTGAACTTGGGGTTGGGAAGGCTGAGCTTGGGATTGTTGAGGATCAATTTCAACTGTTTGTTGTTCTTGTTGATCTGAGATTCTTTGTAATTGCTCAGCAATCTGTTCTTGGCGTTCCTTCTCTTCAAGTAAACGTTTTTCTTTGCGCTTTACAATAG", "aa": "MQVLKAEHAVETVVEGVVELGVGKAELGIVEDQFQLFVVLVDLRFFVIAQQSVLGVPSLQVNVFLCALQ", "aa_hexdigest": "2c2914b64d56c59a9343b0f1daf73d56", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7510.7654999999995, "isoelectric_point": 4.375886344909667 }, "id": "KMADJIBOEG_737", "locus": "GCA000292445_00722" }, { "type": "cds", "sequence": "contig_1", "start": 397592, "stop": 397795, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGTTAAATGGATTTTTGAGATACAAAAAACTTTTTATAGTTCTTGCTTTACTTTTTACAACAATCCTAATTGTTAGTTTATCGTTACTAGCATTTGCACTTGTTGTGAAAACTAATGGTAGTGAACTAGGGGTTGTTTTTCATCAAACAGAAGATAATACAACTGTAATTCAGGGCAGATCAATTGTTGAACAGCCCTGA", "aa": "MELNGFLRYKKLFIVLALLFTTILIVSLSLLAFALVVKTNGSELGVVFHQTEDNTTVIQGRSIVEQP", "aa_hexdigest": "9d7993b6226778def9d21e86b92e2024", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7450.733800000002, "isoelectric_point": 6.532982826232909 }, "id": "KMADJIBOEG_738", "locus": "GCA000292445_00723" }, { "type": "cds", "sequence": "contig_1", "start": 397812, "stop": 398348, "strand": "-", "frame": 2, "gene": null, "product": "MG319/MPN454 family protein", "db_xrefs": [ "GO:0005886", "RefSeq:WP_010869428.1", "SO:0001217", "UniParc:UPI0000139608", "UniRef:UniRef100_P47561", "UniRef:UniRef50_P47561", "UniRef:UniRef90_P47561" ], "nt": "ATGCGCTTGTTTCGATTTCTCTTTAAGCTTTGCTTTCTTTTATTGGTATTGGTAGGCTTTGCTTATCTTTTCTTAGCTATCTTTTACTTTGGTTCTCTAAACCCATTTGAGTTAGCACAACCAATGGATGTTTTCAACAGGTTCTTTTCAAAAGAAGCGTTGGATAACATCTCTTCAAACAATGGTGCAACTGCAACTGCTCAAACAAGTAGCTTATTACAACTTTTAGAAGGTTCAAGTAATGGTTTGGATAACCGTTTTCCAACTGAAAAAAGTGCATTTTATGCTATCCCAGGATATGTAGATTTTTTGAAAAATGCTAAGTTACCTGGATTTGTTGAACAGTTTACACCTTATCTAACTAAGTATGTAATTCCCCTAGGAATGGCATTTGTTAGTGGTTTAATAGGTACTTTAATTGTTAACTTCTTTCTCAATAAAATCACTCGTTCAATTAAAAGAAGAAAAAGGAACATGAAAAAGCAAGAACAAGAAGAGTATTATGATGATTCAAGATCAAGAAGAAAAAGGAACTAA", "aa": "MRLFRFLFKLCFLLLVLVGFAYLFLAIFYFGSLNPFELAQPMDVFNRFFSKEALDNISSNNGATATAQTSSLLQLLEGSSNGLDNRFPTEKSAFYAIPGYVDFLKNAKLPGFVEQFTPYLTKYVIPLGMAFVSGLIGTLIVNFFLNKITRSIKRRKRNMKKQEQEEYYDDSRSRRKRN", "aa_hexdigest": "b27dbdd9f01959166de9f05ca322577a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139608", "ncbi_nrp_id": "WP_010869428.1", "uniref100_id": "UniRef100_P47561", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139608", "RefSeq:WP_010869428.1", "UniRef:UniRef100_P47561" ] }, "ips": { "uniref100_id": "UniRef100_P47561", "uniref90_id": "UniRef90_P47561" }, "psc": { "uniref90_id": "UniRef90_P47561", "product": "MG319/MPN454 family protein", "uniref50_id": "UniRef50_P47561", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47561", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47561" ], "product": "Uncharacterized protein MG319" }, "genes": [], "id": "KMADJIBOEG_739", "locus": "GCA000292445_00724" }, { "type": "cds", "sequence": "contig_1", "start": 398557, "stop": 398808, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTCACATCTTATTATGGGCTTTTGTAATTAGTATTGTAGCGAGCTTATTGAGCTTTATTTTGAACTTTACAGTCTTTGCTTCTACAACAAACCTACTTGATAGAATTATCCCTGGCTTATCTTTAATAGTAGGGGGAATCTTTTCATTAATTTCAGTTTATTTTGTGAGTTTACAGATCCGTAATGAACAAGATTTGATTAAATACTATGAAAGTGAAGATTATGAAATGGCAAGAAGACAGAGCTGA", "aa": "MFHILLWAFVISIVASLLSFILNFTVFASTTNLLDRIIPGLSLIVGGIFSLISVYFVSLQIRNEQDLIKYYESEDYEMARRQS", "aa_hexdigest": "661d40e5410c0b23eadfa555bff4be3f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9552.070799999996, "isoelectric_point": 5.045847511291503 }, "id": "KMADJIBOEG_740", "locus": "GCA000292445_00725" }, { "type": "cds", "sequence": "contig_1", "start": 398860, "stop": 399312, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MG320", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47562", "UniRef:UniRef90_P47562" ], "nt": "ATGATTAATTCAACAAAAGGCTATATTGATCAAAACGGTTTAACAGCAAAGCAGTTTGTACAAACAAAACAGCTTTCTGTAATCCGTTTAACTTTTATGGTAGCTGCTTTTGGGATCTTTTTTATCTTTCTTGTTGCATTAACAGTACAACAATTGCTTTCAAGAAGCACACTAATTGATTTAGCTAGTGATTTTAGAACACTTAGTACTATTGCAGTTATTACTAGTTTTGTCTCTTTAATTCTTTATTTTGTTACTGCTTTTAAATTAAGGAATCCCAACACTAGTTTGACATGGTTTTGGGCCTTAATCATTACTGATGTGATTAGTTATGGGATAACCCTTGGGATCTTATTAACTTTAGCAACAACATTTTCAAAACAAGTTAACTTTGAAGCTAATGACATTGTTTATGCTTTTTTAGGTGCTAGTTTGGTGTTTGGTTCAGTGTGA", "aa": "MINSTKGYIDQNGLTAKQFVQTKQLSVIRLTFMVAAFGIFFIFLVALTVQQLLSRSTLIDLASDFRTLSTIAVITSFVSLILYFVTAFKLRNPNTSLTWFWALIITDVISYGITLGILLTLATTFSKQVNFEANDIVYAFLGASLVFGSV", "aa_hexdigest": "803c49f298d7648966de02e408e33bad", "start_type": "ATG", "rbs_motif": "ATTTA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 398557, "stop": 399312, "observations": { "mutation": [ 398862 ], "stop": [ 398862 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47562", "query_cov": 1.0, "subject_cov": 0.5244755244755245, "identity": 0.993, "score": 272.0, "evalue": 4.62e-90, "gene_start": 1, "gene_end": 150, "reference_start": 1, "reference_end": 150, "reference_sequence": "MINSTKGYIDQNGLAAKQFVQTKQLSVIRLTFMVAAFGIFFIFLVALTVQQLLSRSTLIDLASDFRTLSTIAVITSFVSLILYFVTAFKLRNPNTSLTWFWALIITDVISYGITLGILLTLATTFSKQVNFEANDIVYAFLGASLVFGSVWGLSALPSQKRRYQQTQTLFHILLWAFVISIVASLLSFILNFTVFASTTNLLDRIIPGLSLIVGGIFSLISVYFVSLQIRNEQDLIKYYESEDYEMARRQSWRSALFFGAWLISSFMNLVYFILRIILITKNFSRV" }, "paralog": false, "identity": 0.9920318725099602, "score": 471.0, "evalue": 3.41e-171, "description": "Internal stop codon at 398862. Nonsense mutation around 398862.", "psc": { "uniref90_id": "UniRef90_P47562", "product": "Uncharacterized protein MG320", "uniref50_id": "UniRef50_P47562", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47562", "UniRef:UniRef50_P47562", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47562", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47562" ], "product": "Uncharacterized protein MG320" } }, "id": "KMADJIBOEG_741", "locus": "GCA000292445_00726" }, { "type": "tRNA", "sequence": "contig_1", "start": 399382, "stop": 399472, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(gga)", "amino_acid": "Ser", "anti_codon": "gga", "score": 70.9, "nt": "GGATACTTACCCAAGTGGCTGAAGGGGTAGGCTTGGAAAGCTTATAGATGGGTAAAACCATGCGAGGGTTCGAATCCCTCAGTATCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 399382, 399384 ], "id": "KMADJIBOEG_13", "locus": "GCA000292445_00727" }, { "type": "tRNA", "sequence": "contig_1", "start": 399474, "stop": 399562, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(cga)", "amino_acid": "Ser", "anti_codon": "cga", "score": 78.7, "nt": "GGAGATTTACCCAAGTGGCTGAAGGGGGCGCTCTCGAAAAGCGTTAGGTGGTTATCCACGCGTGGGTTCAAATCCCACAATCTCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 399508, 399510 ], "id": "KMADJIBOEG_14", "locus": "GCA000292445_00728" }, { "type": "cds", "sequence": "contig_1", "start": 399818, "stop": 400222, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGCTGCATCCCAAAACAATGTCTTTAATCAAATTGAAAATAAACTTCAAAATAACACAACAACTAACGGAAAAGACTGGTATAGTTGCATAACAAGTGCTGATCTTTTTAAAGATGATCCCTATGTCATTAAGAACTTTGGTACTAATGGTAATAACGGTACATCCGCTTCATTAGCATTCACTAAAAAAGCATTAAGCTTACTGAAATTTTTAGTTGATAATAAGGTATTGAAAGCTGATAAAGTTAAAGAAGCAATTAAAGATCCTGATAAATATCTATCAAAAAGAAGCAAGATAGATGACAACAAACCAACAAATGTATCAGATATCCACATTGGTTATGAATTGAAAGATCTTTATGACAACGCTGCTCAACTTAATCGCTTTAACAGCATTTGA", "aa": "MLAASQNNVFNQIENKLQNNTTTNGKDWYSCITSADLFKDDPYVIKNFGTNGNNGTSASLAFTKKALSLLKFLVDNKVLKADKVKEAIKDPDKYLSKRSKIDDNKPTNVSDIHIGYELKDLYDNAAQLNRFNSI", "aa_hexdigest": "2f064d7ab1264ff6ddb689d2e3838f43", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15039.732799999982, "isoelectric_point": 8.887756538391116 }, "id": "KMADJIBOEG_742", "locus": "GCA000292445_00729" }, { "type": "cds", "sequence": "contig_1", "start": 401411, "stop": 402433, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAAAAAAAAGATACTTGTTGCTCGGTTCTACACTGACAGTTAGTGCAGCACTAATCCTAAGTGCTTGTGCATCCTCACAGTTAAACAAAGGGGTGTTTTTCACCTCTTCTTCAGCAGATTTGTTAAAGAACAACTCTGTTCCATTGAGCATGTTTAACGTTTCCCCCACTTCATCTTTTTTTGGTAATGCTTATGCAGGGTTAACTAACTATGTTGCTACTGGTACAAACCGTGATGATGGGGTTAATGTTACTGACCAAATTAAAGAAAAATTAGTGTTAGAACTTGCTTTATCAGTTACTGGCTATAAAAAGACAACACACAGTGGTCTTAAAGGTAGAGCACAAAAAAATGGTTCAACAGGTTCTTCTAATGGATCTAGTAAGAATGATTACACTAAGTTCAATGAGTGGGATGCAATAGGCACAATTTATCGTGATAGTTCAAAGTCTATTACTGAAGATCCTAACTATCACAAGATAACCCAAGAAGCAACCAGATATGAATTTACCATTAACACTAGTTTGAACTGGGTTGATAATGCTGGTAGAGAAGTCAAACAAAACAACCAACCAGTTAAACTCTCCTCTAAAGACTTTGAAAGGGGTTTTGAGACCTATATTCTCTCTTCTAACTTAGGTTTTAACCGGAATGGTTATTTTATTGACCTAATGGGTCTTGATGTTGAAAAAACCGTTGGTATGGATAAAACCAACGGTTCTAGTGGTAACAATGGTAAAGGTATTGAGATCACTGATGAAAATTATGATGTTGAAAACTATAGAAGTGTAGATGATAACAAGTTTAATGTTTATCTAACTTCTCCTTTTCCTTTCTTTCTATCAATGATGTCAAAGGAATTCTTCTTTCCAATTCCTCACACCCATCCTAAGGTAAAAGCCCTTAAACTAGGTAAAGATTCACCGCTTAAATATAACCAAAATAACAACAGAAAGATTTTAGATCAAGCAAATACTAACTTTGATGGTATCTATGGAGGGGGTGTTAATGCTTGA", "aa": "MKLKKRYLLLGSTLTVSAALILSACASSQLNKGVFFTSSSADLLKNNSVPLSMFNVSPTSSFFGNAYAGLTNYVATGTNRDDGVNVTDQIKEKLVLELALSVTGYKKTTHSGLKGRAQKNGSTGSSNGSSKNDYTKFNEWDAIGTIYRDSSKSITEDPNYHKITQEATRYEFTINTSLNWVDNAGREVKQNNQPVKLSSKDFERGFETYILSSNLGFNRNGYFIDLMGLDVEKTVGMDKTNGSSGNNGKGIEITDENYDVENYRSVDDNKFNVYLTSPFPFFLSMMSKEFFFPIPHTHPKVKALKLGKDSPLKYNQNNNRKILDQANTNFDGIYGGGVNA", "aa_hexdigest": "38afe062f9c2d536712228a5244fa71e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 37611.541200000014, "isoelectric_point": 8.942103385925293 }, "id": "KMADJIBOEG_743", "locus": "GCA000292445_00730" }, { "type": "tRNA", "sequence": "contig_1", "start": 402644, "stop": 402728, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(tag)", "amino_acid": "Leu", "anti_codon": "tag", "score": 64.0, "nt": "GCACTCGTGGCGGAATGGTAGACGCGCTAGACTTAGGATCTAGTTTCATCTAGAAGTGGGGGTTCAAGTCCCTTCGGGTGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 402708, 402710 ], "id": "KMADJIBOEG_25", "locus": "GCA000292445_00731" }, { "type": "tRNA", "sequence": "contig_1", "start": 402734, "stop": 402809, "strand": "-", "gene": "trnK", "product": "tRNA-Lys(ctt)", "amino_acid": "Lys", "anti_codon": "ctt", "score": 81.4, "nt": "GCATCTTTAGCTCAGTTGGTAGAGCAAATGACTCTTAATCATTGGGTCGTGGGTTCGATCCCCTCAAGATGCACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 402803, 402805 ], "id": "KMADJIBOEG_24", "locus": "GCA000292445_00732" }, { "type": "tRNA", "sequence": "contig_1", "start": 402816, "stop": 402891, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(tgt)", "amino_acid": "Thr", "anti_codon": "tgt", "score": 90.9, "nt": "GCCGACTTAGCTCAGTTGGTAGAGCAATTGACTTGTAATCAATAGGTCGTAGGTTCAAATCCTATAGTCGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 402856, 402858 ], "id": "KMADJIBOEG_23", "locus": "GCA000292445_00733" }, { "type": "tRNA", "sequence": "contig_1", "start": 402893, "stop": 402968, "strand": "-", "gene": "trnV", "product": "tRNA-Val(tac)", "amino_acid": "Val", "anti_codon": "tac", "score": 81.7, "nt": "GGATTGTTAGCTCAGCTGGGAGAGCATATGCCTTACAAGCATAGGGTCGGGGGTTCGATCCCCTCACAATCCACCA", "db_xrefs": [ "SO:0000273" ], "anti_codon_pos": [ 402933, 402935 ], "id": "KMADJIBOEG_22", "locus": "GCA000292445_00734" }, { "type": "tRNA", "sequence": "contig_1", "start": 402977, "stop": 403051, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(cgt)", "amino_acid": "Thr", "anti_codon": "cgt", "score": 80.2, "nt": "GCCGAATTAGCTCAAGGGCAGAGCGATGCACTCGTAATGCATAGGCTACAGGTTCAATTCCTGTATTCGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 403017, 403019 ], "id": "KMADJIBOEG_21", "locus": "GCA000292445_00735" }, { "type": "tRNA", "sequence": "contig_1", "start": 403054, "stop": 403130, "strand": "-", "gene": "trnE", "product": "tRNA-Glu(ttc)", "amino_acid": "Glu", "anti_codon": "ttc", "score": 58.9, "nt": "GGCGCGTTGGTGAAGAGACTTAACACACACGCCTTTCACGCGTGCATTCACGGGTTTGAATCCCGTACGCGTCACCA", "db_xrefs": [ "SO:0000259" ], "anti_codon_pos": [ 403094, 403096 ], "id": "KMADJIBOEG_20", "locus": "GCA000292445_00736" }, { "type": "tRNA", "sequence": "contig_1", "start": 403137, "stop": 403211, "strand": "-", "gene": "trnN", "product": "tRNA-Asn(gtt)", "amino_acid": "Asn", "anti_codon": "gtt", "score": 84.0, "nt": "GGCCACATAGCTCAGCGGTAGAGCAACCGGCTGTTAACCGGTTGGTCACAGGTTCGATCCCTGTTGTGGCCGCCA", "db_xrefs": [ "SO:0000257" ], "anti_codon_pos": [ 403177, 403179 ], "id": "KMADJIBOEG_19", "locus": "GCA000292445_00737" }, { "type": "cds", "sequence": "contig_1", "start": 403233, "stop": 404543, "strand": "-", "frame": 2, "gene": "trkG", "product": "putative cation transporter MG322", "db_xrefs": [ "COG:COG0168", "COG:P", "GO:0005886", "GO:0008324", "GO:0030001", "SO:0001217", "UniRef:UniRef50_P75323", "UniRef:UniRef90_P47564" ], "nt": "TTGTTTAACTTTCACAAGAAGGAACAATACAGTTTTTATGAAAAGTTAATGTTGCAATCAGAACGAGGTGGTTCTAAGCTAGGTAATACTAGTGAGATGATCTTAGTATCTATCATCTTTCTGTTTATCGTTGAACTAATTTATGGATTTTTATATGGTATTTTGTTTTATTTCATCCCAGGCTTTGAACCTGCTAACTTGTTTGCAGATCATGCAAAAGTTTCAACTCAATTAAAAGCTTTAGTAGTTGATTCAAACCAAACAATAGCAGGTTTTAATGATATTAATAAGGCTTTTCAAGCAGGTTTTTTCCATTCCTTATCAGCAGTTAATAATGCTGGGATAGATCTGATAGGGGGTAGTTCTTTTGTTCCTTATAGAAATGGACTTGGTATTATTATCCAGTGGTTAACTATTAGCCAAATTATCTTTGGGGGAATTGGTTATCCTTGTTTGTTTGATGGCTTTGAAGCCATTAAAAAAAAGATTAAGTATGGTAGACACACAAAACACCAATTTAGTCTATTTACCAAGTTAACAGTAATTACTAATATCGTTGTAATCCTGCTTTTTTTCACCTTACTTTTAATGGTGGAATTTATTGCTAGTGATAGTTTAACTAACACTATTGTTAATTTTAGTGATGAAAAAAAGAGTTTAATAAATACCCAATTGCAATCACAATCTAACCAAGCAATCCATGCGTCAGTTTTTGGTAATAACCCTAATGCAAGTAGGGTAATGCAGCTCTTTTTTATGGTTATTTCATCCCGTTCAGCAGGTTTTAGTGTTTTCCCTGTTGCTAGTGAGAATCAAACTACAAAAATAATTATTGCATTAACAATGTTTATTGGTGCTAGTCCCTCTTCTACTGCTGGGGGGATTAGAACAACTACGCTAGCAGTGATCTTTTTAGCTTTAGTTGCTAAGTTTAAAGGTCAAAAGGAAGTAAAAGCATTTAAACGTTCAATCGATCAAACTACAGTAATAGATGCTTTTTTAGTACTAATTTTAAGTTTAATTGCAGTTTTACTAACAGCTGTTCTTCTACCTTTAAGTATGGAACAACCAGTTAGTTTCATTGATGCTTTATTTGAAACAACTAGTGCTTTTGGAACAGTTGGACTTTCAAGTGGAGCTACTGTTAACATTGCTTTAGATCCAAATAGAAATACCTTTAATTTCCTTGCTTTATGTCTATTAATGGTTATGGGACAGGTTGGTGTGTCCAGTTCTGTGCTAACTTTTGTTAGAAAACATCCCAAAGCAAATAGTTATTCATATCCTAAGGAAGCTGTTAAAATTGGCTAG", "aa": "MFNFHKKEQYSFYEKLMLQSERGGSKLGNTSEMILVSIIFLFIVELIYGFLYGILFYFIPGFEPANLFADHAKVSTQLKALVVDSNQTIAGFNDINKAFQAGFFHSLSAVNNAGIDLIGGSSFVPYRNGLGIIIQWLTISQIIFGGIGYPCLFDGFEAIKKKIKYGRHTKHQFSLFTKLTVITNIVVILLFFTLLLMVEFIASDSLTNTIVNFSDEKKSLINTQLQSQSNQAIHASVFGNNPNASRVMQLFFMVISSRSAGFSVFPVASENQTTKIIIALTMFIGASPSSTAGGIRTTTLAVIFLALVAKFKGQKEVKAFKRSIDQTTVIDAFLVLILSLIAVLLTAVLLPLSMEQPVSFIDALFETTSAFGTVGLSSGATVNIALDPNRNTFNFLALCLLMVMGQVGVSSSVLTFVRKHPKANSYSYPKEAVKIG", "aa_hexdigest": "8f254f52ba8c73adccf73d91e80f146c", "start_type": "TTG", "rbs_motif": null, "genes": [ "trkG" ], "truncated": "3-prime", "pseudogene": { "start": 403233, "stop": 404843, "observations": { "mutation": [ 404549, 404744 ], "stop": [ 404549, 404744 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47564", "query_cov": 1.0, "subject_cov": 0.7716814159292036, "identity": 0.9890000000000001, "score": 802.0, "evalue": 2.75e-290, "gene_start": 1, "gene_end": 436, "reference_start": 130, "reference_end": 565, "reference_sequence": "MTRRQNQMVKLTTWLKKIGWGETITQRIFCFYIYCILFGSLLLFLPIALQDNYQKVVSYGIDWQGKRFEQKTDYNFLDALFLSTSAFSDTGLSTVVVSKTYSIFGQIVLAVLLQLGGIGFVVIAFLAWRLFNFHKKEQYSFYEKLMLQSERGGSKLGNTSEMILVSIIFLFIVELIYGFLYGILFYFIPGFEPANLFADHAKVSTQLKALVVDSNQTIAAFNDINKAFQAGFFHSLSAVNNAGIDLIGGSSFVPYRNGLGIIIQWLTISQIIFGGIGYPCLFDGFEAIKKKIKYGRHTKHQFSLFTKLTVITNIVVILLFFTLLLMVEFIASDSLTNTIVNFSDEKKSLINTQLQSQSNQAIHASVFGNNPNASRVMQLFFMVISSRSAGFSVFPVASEIQTTKIIIALAMFIGASPSSTAGGIRTTTLAVIFLALVAKFKGQKEVKAFKRSIDQTTVIDAFLVLIISLIAVLLTAVLLPLSMEQPVSFIDALFETTSAFGTVGLSSGATVNIALDPNRNTFNFLALCLLMVMGQVGVSSSVLTFVRKHPKANSYSYPKEAVKIG" }, "paralog": false, "identity": 0.9869402985074627, "score": 1014.0, "evalue": 0.0, "description": "Internal stop codon at 404549, 404744. Nonsense mutation around 404549, 404744.", "psc": { "uniref90_id": "UniRef90_P47564", "gene": "TrkG", "product": "Uncharacterized cation transporter MG322", "uniref50_id": "UniRef50_P75323", "cog_id": "COG0168", "cog_category": "P", "go_ids": [ "GO:0005886", "GO:0008324", "GO:0030001" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47564", "UniRef:UniRef50_P75323", "COG:COG0168", "COG:P", "GO:0005886", "GO:0008324", "GO:0030001" ] }, "pscc": { "uniref50_id": "UniRef50_P75323", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75323" ], "product": "Uncharacterized cation transporter MG322 homolog" } }, "id": "KMADJIBOEG_744", "locus": "GCA000292445_00738" }, { "type": "cds", "sequence": "contig_1", "start": 404966, "stop": 405649, "strand": "+", "frame": 2, "gene": "trkA", "product": "putative protein MG323", "db_xrefs": [ "COG:COG0569", "COG:PT", "GO:0006813", "GO:0008324", "RefSeq:WP_014894289.1", "SO:0001217", "UniParc:UPI00027B3F55", "UniRef:UniRef100_UPI00027B3F55", "UniRef:UniRef50_P75322", "UniRef:UniRef90_P47565" ], "nt": "ATGAAACGTGCAGATTTTTGCATTATTGGTTTAGGTAGATTTGGGATGCAGGTTGCACAATCACTAAAAGAAAACAACTTTAACTTACTTTTAATTGATCTTGATGATAAAAAAACTGACACCGCCTCACAACAGTTTGATTATGTTATTTGTTGTGATGCTAGTAATCTAACTGCTTTAGAAGAGTTACAAATTGATGAGTTTGCTGGGGTTATTGTTGGGGTTACCAACATAGAAGCGAGCATCATGATATGTGCTAATTTAAGGGAATTAGGACAAAAAAACATTATTGCTAAAGCCAAAAATGAAGTACATAAAAGGGTGTTAAGCACAATGGGAATTAGAGAAGCTTTGATCCCTGAAAAGATTGTTGGTAAAAATCTGGTTATCCGCTTAATCCATGGGCTTGAAAATGAAATTATCAACCTTGGTAATGAGATAATTTTCATCCGTTCAGCAGTTAACAACAAGGCTTTTTTTAACAAAAGGTTAGGAGAGATTAACTTTAGGCAAAACACCGATGCTAACATTATCTCCATCATGCGCAGTAATAAAACTGTTGTTTTTCCTTTAGGACCAAATACTGAGATCCAACCAGGGGATATTATCACTGCAGTTTGTCAACAAAAAAGTTTAAATAAGTACTTAAATTACATCAATCCTAAAACCAAAAATAAAAATTAA", "aa": "MKRADFCIIGLGRFGMQVAQSLKENNFNLLLIDLDDKKTDTASQQFDYVICCDASNLTALEELQIDEFAGVIVGVTNIEASIMICANLRELGQKNIIAKAKNEVHKRVLSTMGIREALIPEKIVGKNLVIRLIHGLENEIINLGNEIIFIRSAVNNKAFFNKRLGEINFRQNTDANIISIMRSNKTVVFPLGPNTEIQPGDIITAVCQQKSLNKYLNYINPKTKNKN", "aa_hexdigest": "f56e4a26e516728bb051508fb9396926", "start_type": "ATG", "rbs_motif": "ATATA", "ups": { "uniparc_id": "UPI00027B3F55", "ncbi_nrp_id": "WP_014894289.1", "uniref100_id": "UniRef100_UPI00027B3F55", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3F55", "RefSeq:WP_014894289.1", "UniRef:UniRef100_UPI00027B3F55" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3F55", "uniref90_id": "UniRef90_P47565" }, "psc": { "uniref90_id": "UniRef90_P47565", "gene": "TrkA", "product": "Uncharacterized protein MG323", "uniref50_id": "UniRef50_P75322", "cog_id": "COG0569", "cog_category": "PT", "go_ids": [ "GO:0008324", "GO:0006813" ] }, "pscc": { "uniref50_id": "UniRef50_P75322", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75322" ], "product": "Uncharacterized protein MG323 homolog" }, "genes": [ "trkA" ], "id": "KMADJIBOEG_745", "locus": "GCA000292445_00739" }, { "type": "ncRNA", "class": [ "RNase_P_RNA", "SO:0000386" ], "sequence": "contig_1", "start": 405689, "stop": 406029, "strand": "-", "gene": "RNaseP_bact_b", "product": "Bacterial RNase P class B", "score": 251.3, "evalue": 2.2e-27, "db_xrefs": [ "RFAM:RF00011", "SO:0000386" ], "nt": "GCTGTCGGTTAGTTGCTGCTTTAAAGATATATTTAAAGTAGAGGAAAGTCCATGCTTGCACTTGCTGGAATGCAAGTAGTGTTTGTGTAAATCAAAGCGATAAGATTTAGGATGTGATAGCCATAACGACATAACTAGTCTAAGGTTAATACTATGATGATGTTTTGAAAGTGCCACAGAGACGAGCTTGGTGAAAACCAGGTATGAAACGCGGTAAACTCCACAAGCAAGAAACCTAAATTTTGGTAAGGGAATGTTGCTTTAAGAACAAATAATTAAAGTAATGACAGCAATTGCTGTAGATAAATAACTGACTTTAAAACAGAACATGGCTTATTGAC", "id": "KMADJIBOEG_41", "locus": "GCA000292445_00740" }, { "type": "tmRNA", "sequence": "contig_1", "start": 406052, "stop": 406439, "strand": "+", "gene": "ssrA", "product": "transfer-messenger RNA, SsrA", "db_xrefs": [ "SO:0000584" ], "tag": { "start": 406135, "stop": 406215, "aa": "DKENNEVLVDPNLIINQQASVNFAFA", "nt": "GACAAAGAAAATAACGAAGTGTTGGTAGATCCAAATTTGATCATTAACCAACAAGCAAGTGTTAACTTTGCTTTTGCATAA" }, "nt": "GGGGATGTTTTGGGTTTGACATAATGCTGATAGACAAACAGTAGCATTGGGGTATGCCCCTTACAGCGCTAGGTTCAATAACCGACAAAGAAAATAACGAAGTGTTGGTAGATCCAAATTTGATCATTAACCAACAAGCAAGTGTTAACTTTGCTTTTGCATAAGTAGATACTAAAGCTACAGCTGGTGAATAGTCATAGTTTGCTAGCTGTCATAGTTTATGACTCGAGGTTAAATCGTTCAATTTAACCTTTAAAAATAGAACTTGTTGTTTCCATGATTGTTTTGTGATCAATTGGAAACAAGACAAAAATCCACAAAACTAAAATGTAGAAGCTGTTTGTTGTGTCCTTTATGGAAACGGGTTCGATTCCCGTCATCTCCACCA", "id": "KMADJIBOEG_37", "locus": "GCA000292445_00741" }, { "type": "cds", "sequence": "contig_1", "start": 406590, "stop": 406748, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTATTTTTGGTGTTATTAGACCAACAGTTTTTTGAAAGAGATACACAAAAAATTCAATCAGCAACGCGGTTTAGCTTGTTTTTTAATGTTGAAAATAACAATGGTGTTTATCTCTCTAAATTAAATAGTTTTGGTGTATTTGCTACCGCTTGA", "aa": "MVLFLVLLDQQFFERDTQKIQSATRFSLFFNVENNNGVYLSKLNSFGVFATA", "aa_hexdigest": "e984ef0aaf7f2c60a8f119f77d0000b3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6009.7965, "isoelectric_point": 5.971413612365722 }, "id": "KMADJIBOEG_746", "locus": "GCA000292445_00742" }, { "type": "cds", "sequence": "contig_1", "start": 406980, "stop": 407249, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTTCTAAAAAGAAAAATAGTGGTGGTGGTTGGATTAATAAGATATCTGATAAACTCAGTGAAATAAATGTCGCTGAGTATCAAGTTGTTTCCAAACACAGTAACTATCTGTTTTACAGCAGGTTTGGCTTACTTGATACTTTTGTTTACTTTTTATTTTTTCTCTGCTTTTTTCTAAACAACGTTTTGTTTTTAGCTGGCGTTTTTCACACTAAACAATTCACTTTTAACGAAGTTAACGGCTTTAATCAGTTTTACCTTTTTTGA", "aa": "MLSKKKNSGGGWINKISDKLSEINVAEYQVVSKHSNYLFYSRFGLLDTFVYFLFFLCFFLNNVLFLAGVFHTKQFTFNEVNGFNQFYLF", "aa_hexdigest": "fd133266959697cd97b6eddd4a3a3c52", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10493.975099999987, "isoelectric_point": 9.131962776184086 }, "id": "KMADJIBOEG_747", "locus": "GCA000292445_00743" }, { "type": "cds", "sequence": "contig_1", "start": 407269, "stop": 407916, "strand": "-", "frame": 1, "gene": "pepP", "product": "Putative Xaa-Pro aminopeptidase", "db_xrefs": [ "COG:COG0006", "COG:E", "EC:3.4.11.9", "GO:0004177", "GO:0005829", "GO:0006508", "GO:0008237", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47566", "UniRef:UniRef90_P47566" ], "nt": "TTGCCTAGTGAGATTCAAGCCATTGAAAAAGCAGTTGATATTACAAGAAAAGTAGCAGTTAAGTTAAAGCGTTTTATTAAACCAAAAATGACTGAGCTTTTCATTTCACAATGGATTACTAATGAGCTTGTGAAGCAGGGTGGGGCTAAAAACTCATTTGATCCTATAGTAGCTACTGGTAAAAACGGTGCTAACCCACATCACAAACCAACCAAAACAATAGTTAAGGAAGGTGATTTTATCACTTGTGATTTTGGCACCATTTACAACGGTTATTGCTCTGATATTACAAGAACCTTTTTAGTTGGTAAAAAACCTAAAAGTGCAAAGTTATTATCTGCATACAAAAAAGTTGAAGAGGCTAATTTGGCTGGTATAAATGCAGTAAACACTACTTTAACAGGTTCACAAGTTGATAAGGTGTGCCGCGATATTATTGAAAACTCTGAGTTTAAAGACTTTTTTGTGCATAGTACTGGTCATGGCGTTGGTATTGATATCCATGAAATGCCAAATGTTTCCCAATCTTACAACAAGTTATTGTGTGAAAATGGGGTTGTAACCATTGAACCTGGGATCTATATTCCTAACCTTGGTGGAATTAGGATAGAAGATATGGTACTTGTTAAAAAAGAAAAATCAGTTTGA", "aa": "MPSEIQAIEKAVDITRKVAVKLKRFIKPKMTELFISQWITNELVKQGGAKNSFDPIVATGKNGANPHHKPTKTIVKEGDFITCDFGTIYNGYCSDITRTFLVGKKPKSAKLLSAYKKVEEANLAGINAVNTTLTGSQVDKVCRDIIENSEFKDFFVHSTGHGVGIDIHEMPNVSQSYNKLLCENGVVTIEPGIYIPNLGGIRIEDMVLVKKEKSV", "aa_hexdigest": "c1439c6283541e84a60c4a4643c85ca5", "start_type": "TTG", "rbs_motif": null, "genes": [ "pepP" ], "truncated": "both", "pseudogene": { "start": 407239, "stop": 408216, "observations": { "mutation": [ 407271, 407979, 408204 ], "stop": [ 407271, 407979, 408204 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47566", "query_cov": 1.0, "subject_cov": 0.6073446327683616, "identity": 0.995, "score": 432.0, "evalue": 4.99e-151, "gene_start": 1, "gene_end": 215, "reference_start": 130, "reference_end": 344, "reference_sequence": "MISELQQKITVLKDLLKTNKADAILIGSDQNRFWLTNFPSSAGWLIITSNKAKLFIDGRYYEAARNFINPIVEVELFVSFKQVKAFCESNGINHLLIEGDYLTFNYQDWIQAICKQYTVINAQEIRRVKLPSEIQAIEKAVDITRKVAVKLKRFIKPKMTELFISQWITNELVKQGGAKNSFDPIVATGKNGANPHHKPTKTIVKEGDFITCDFGTIYNGYCSDITRTFLVGKKPKSAKLLSAYKKVEEANLAGINAVNTTLTGSQVDKVCRDIIENSEFKDFFVHSTGHGVGIDIHEMPNVSQSYNKLLCENGVVTIEPGIYIPNLGGIRIEDMVLVKKEKSVWLSKSIPRAF" }, "paralog": false, "identity": 0.9907692307692307, "score": 641.0, "evalue": 5.33e-236, "description": "Internal stop codon at 407271, 407979, 408204. Nonsense mutation around 407271, 407979, 408204.", "psc": { "uniref90_id": "UniRef90_P47566", "gene": "pepP", "product": "Putative Xaa-Pro aminopeptidase", "ec_ids": [ "3.4.11.9" ], "uniref50_id": "UniRef50_P47566", "cog_id": "COG0006", "cog_category": "E", "go_ids": [ "GO:0005829", "GO:0004177", "GO:0046872", "GO:0008237", "GO:0006508" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47566", "EC:3.4.11.9", "UniRef:UniRef50_P47566", "COG:COG0006", "COG:E", "GO:0005829", "GO:0004177", "GO:0046872", "GO:0008237", "GO:0006508" ] }, "pscc": { "uniref50_id": "UniRef50_P47566", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47566" ], "product": "Putative Xaa-Pro aminopeptidase" } }, "id": "KMADJIBOEG_748", "locus": "GCA000292445_00744" }, { "type": "cds", "sequence": "contig_1", "start": 407977, "stop": 408144, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTATTGATGGTAGGTATTATGAAGCAGCAAGGAACTTTATTAATCCTATTGTTGAAGTTGAATTATTTGTTAGTTTTAAACAAGTTAAAGCTTTTTGTGAATCTAATGGTATCAATCACCTTTTAATTGAGGGTGACTATCTTACCTTTAACTATCAGGATTGA", "aa": "MFIDGRYYEAARNFINPIVEVELFVSFKQVKAFCESNGINHLLIEGDYLTFNYQD", "aa_hexdigest": "b613da37f195ebf056763eb64660f9e1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6483.252399999999, "isoelectric_point": 4.63461742401123 }, "id": "KMADJIBOEG_749", "locus": "GCA000292445_00745" }, { "type": "cds", "sequence": "contig_1", "start": 408202, "stop": 408303, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAGTGAATTACAACAAAAAATTACAGTTCTTAAAGATCTTTTAAAAACCAATAAAGCTGATGCTATCCTAATTGGTTCTGATCAAAACCGCTTTTGA", "aa": "MISELQQKITVLKDLLKTNKADAILIGSDQNRF", "aa_hexdigest": "630404c05c9db6326146da9695555f66", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3745.3478999999998, "isoelectric_point": 8.189692878723147 }, "id": "KMADJIBOEG_750", "locus": "GCA000292445_00746" }, { "type": "cds", "sequence": "contig_1", "start": 408303, "stop": 408464, "strand": "-", "frame": 2, "gene": "rpmG", "product": "50S ribosomal protein L33", "db_xrefs": [ "COG:COG0267", "COG:J", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_009885961.1", "SO:0001217", "UniParc:UPI0000132FB9", "UniRef:UniRef100_P47567", "UniRef:UniRef50_P47567", "UniRef:UniRef90_P47567" ], "nt": "ATGGCTGTTAAAAGAAGCACACGACTAGGTTGTAATGAATGTTCTGAGATTAATTATTTAACCTTTAAAAACGTCAAGAAAAATCCAGAAAAACTAGCACTTAATAAGTTTTGTTCACGTTGTCGTAAGGTAGTATTGCACAAAGAGGTAAAACGTAAATAA", "aa": "MAVKRSTRLGCNECSEINYLTFKNVKKNPEKLALNKFCSRCRKVVLHKEVKRK", "aa_hexdigest": "57bb09256735e72ca12fdf19d5cbdcfd", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000132FB9", "ncbi_nrp_id": "WP_009885961.1", "uniref100_id": "UniRef100_P47567", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000132FB9", "RefSeq:WP_009885961.1", "UniRef:UniRef100_P47567" ] }, "ips": { "uniref100_id": "UniRef100_P47567", "uniref90_id": "UniRef90_P47567" }, "psc": { "uniref90_id": "UniRef90_P47567", "gene": "rpmG", "product": "50S ribosomal protein L33", "uniref50_id": "UniRef50_P47567", "cog_id": "COG0267", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P47567", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47567" ], "product": "Large ribosomal subunit protein bL33A" }, "genes": [ "rpmG" ], "id": "KMADJIBOEG_751", "locus": "GCA000292445_00747" }, { "type": "cds", "sequence": "contig_1", "start": 408481, "stop": 408975, "strand": "-", "frame": 1, "gene": "degV", "product": "DegV domain-containing protein MG326", "db_xrefs": [ "COG:COG1307", "COG:I", "GO:0008289", "SO:0001217", "UniRef:UniRef50_P75312", "UniRef:UniRef90_P47568" ], "nt": "TTGGTCGAAGATATAAAGGCATTGGTTGACAAGGGTTGTGATAATCAAACAATTAAAGCAAAGGTTGAATCACATAAACAAAATATCCTTTCTGCAGTTACTTTAAAAAACCTAGTGCAAATGCGTAAAGGTGGGAGAATCTCTGGTTTGAAAAAGTTCATTACTACTTTACTAAGAGTTAAACCTATTATCTTGTTTGACAAGGGAGTTAATACTCTAGGAGCAAAAGTTTTTAGTTTTAGTCAAGCGGTTGAGAAGATATTTGGTTTTGTTAAAACTAAGTTTGGTGATAACTACAAAATTAAACGGATCGGTTTTTGCTATTCTTTTTGTAAAAATTACGCTAATGAAATTAAAAAAATAATTACTGATTTCATTGAGCATAATAAGATTAACTTTCAAAATGAAATTGAAAATGCTTTCATTACAAGTGTGATTATTGTTCACACTGGCATTGATGCTTTTTCTATTAGTTTACTTATTGACAATAAATAA", "aa": "MVEDIKALVDKGCDNQTIKAKVESHKQNILSAVTLKNLVQMRKGGRISGLKKFITTLLRVKPIILFDKGVNTLGAKVFSFSQAVEKIFGFVKTKFGDNYKIKRIGFCYSFCKNYANEIKKIITDFIEHNKINFQNEIENAFITSVIIVHTGIDAFSISLLIDNK", "aa_hexdigest": "a4cdaab366d37b9349a46bdab2e1a3b7", "start_type": "TTG", "rbs_motif": null, "genes": [ "degV" ], "truncated": "3-prime", "pseudogene": { "start": 408481, "stop": 409275, "observations": { "mutation": [ 408978 ], "stop": [ 408978 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47568", "query_cov": 1.0, "subject_cov": 0.5559322033898305, "identity": 0.9940000000000001, "score": 318.0, "evalue": 4.44e-108, "gene_start": 1, "gene_end": 164, "reference_start": 132, "reference_end": 295, "reference_sequence": "MKKTAIITDSTASIKPGEINGVYILPLQVIVDGEKSFRDGIEIDYDHVHKLLKENPHGLNISTSLPRQSDLLKIFEEIKTKYDRFIFLPLSKGLSGTYDMLVQLAKELSEQNKDKEFLVFETSDIAISLKWLVEDIKALVDKGCDNQTIKAKVESHKQNILSAVTLKNLVQMRKGGRISGLKKFITTLLRVKPIILFDKGVNTLGAKVFSFSQAVEKIFGFVKTKFGDNYKIKRIGFCYSFCKNYANEIKKIITDFIEHNKINFQNEIENAFITSVIIVHTGIDAFSISLLIDNK" }, "paralog": false, "identity": 0.9962121212121212, "score": 516.0, "evalue": 2.54e-188, "description": "Internal stop codon at 408978. Nonsense mutation around 408978.", "psc": { "uniref90_id": "UniRef90_P47568", "gene": "DegV", "product": "DegV domain-containing protein MG326", "uniref50_id": "UniRef50_P75312", "cog_id": "COG1307", "cog_category": "I", "go_ids": [ "GO:0008289" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47568", "UniRef:UniRef50_P75312", "COG:COG1307", "COG:I", "GO:0008289" ] }, "pscc": { "uniref50_id": "UniRef50_P75312", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75312" ], "product": "DegV domain-containing protein MG326 homolog" } }, "id": "KMADJIBOEG_752", "locus": "GCA000292445_00748" }, { "type": "cds", "sequence": "contig_1", "start": 408976, "stop": 409368, "strand": "-", "frame": 1, "gene": "degV", "product": "DegV domain-containing protein MG326", "db_xrefs": [ "COG:COG1307", "COG:I", "GO:0008289", "SO:0001217", "UniRef:UniRef50_P75312", "UniRef:UniRef90_P47568" ], "nt": "ATGAAGAAAACAGCTATCATCACTGATTCAACTGCTTCTATTAAACCTGGGGAGATTAACGGCGTTTACATCTTGCCTTTACAAGTAATTGTTGATGGTGAAAAAAGCTTTCGTGATGGGATAGAAATTGATTATGATCATGTTCATAAACTTCTAAAAGAAAACCCACATGGACTTAACATCTCAACTTCCTTACCACGTCAAAGTGATCTTCTTAAGATCTTTGAAGAGATTAAAACTAAGTATGATCGCTTTATTTTTCTGCCTTTAAGTAAAGGTTTAAGTGGTACTTATGATATGTTGGTTCAATTAGCAAAAGAGCTTAGTGAACAAAATAAAGACAAAGAATTTTTAGTGTTTGAAACTAGTGATATTGCTATTTCTCTAAAATGA", "aa": "MKKTAIITDSTASIKPGEINGVYILPLQVIVDGEKSFRDGIEIDYDHVHKLLKENPHGLNISTSLPRQSDLLKIFEEIKTKYDRFIFLPLSKGLSGTYDMLVQLAKELSEQNKDKEFLVFETSDIAISLK", "aa_hexdigest": "3bb653f8ccc2d08412055983b432fbcb", "start_type": "ATG", "rbs_motif": null, "genes": [ "degV" ], "truncated": "5-prime", "pseudogene": { "start": 408673, "stop": 409368, "observations": { "mutation": [ 408978 ], "stop": [ 408978 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47568", "query_cov": 1.0, "subject_cov": 0.4406779661016949, "identity": 1.0, "score": 257.0, "evalue": 2.29e-84, "gene_start": 1, "gene_end": 130, "reference_start": 1, "reference_end": 130, "reference_sequence": "MKKTAIITDSTASIKPGEINGVYILPLQVIVDGEKSFRDGIEIDYDHVHKLLKENPHGLNISTSLPRQSDLLKIFEEIKTKYDRFIFLPLSKGLSGTYDMLVQLAKELSEQNKDKEFLVFETSDIAISLKWLVEDIKALVDKGCDNQTIKAKVESHKQNILSAVTLKNLVQMRKGGRISGLKKFITTLLRVKPIILFDKGVNTLGAKVFSFSQAVEKIFGFVKTKFGDNYKIKRIGFCYSFCKNYANEIKKIITDFIEHNKINFQNEIENAFITSVIIVHTGIDAFSISLLIDNK" }, "paralog": false, "identity": 0.9956709956709957, "score": 446.0, "evalue": 2.42e-161, "description": "Internal stop codon at 408978. Nonsense mutation around 408978.", "psc": { "uniref90_id": "UniRef90_P47568", "gene": "DegV", "product": "DegV domain-containing protein MG326", "uniref50_id": "UniRef50_P75312", "cog_id": "COG1307", "cog_category": "I", "go_ids": [ "GO:0008289" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47568", "UniRef:UniRef50_P75312", "COG:COG1307", "COG:I", "GO:0008289" ] }, "pscc": { "uniref50_id": "UniRef50_P75312", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75312" ], "product": "DegV domain-containing protein MG326 homolog" } }, "id": "KMADJIBOEG_753", "locus": "GCA000292445_00749" }, { "type": "cds", "sequence": "contig_1", "start": 409381, "stop": 410058, "strand": "-", "frame": 1, "gene": "menH", "product": "Putative esterase/lipase 2", "db_xrefs": [ "COG:COG0596", "COG:HR", "EC:3.1.-.-", "GO:0052689", "SO:0001217", "UniRef:UniRef50_P75311", "UniRef:UniRef90_Q49418" ], "nt": "TTGTTTAAAAAGAAAAAGTGGCCTTTCTTTACTTTTAATTTTCCCGGTCATGGAGACAATGAATCAACAGATACAGATCAATTAAAACTAAACCACTTTGTTGATTTGGTTTGTGATTTTATTGTTCAAAAAAAACTAAATAACGTCATTTTAATAGGTCATAGTATGGGAGGTGCTGTGGCTGTTTTAGTTAACAAAGTTATACCACTAAAAATTAAGGCTTTAATATTAGTAGCTCCTATGAATCAAACTTCTTTCTCAGTTAATAAAAAGCGGATTTTAGATACTTTTTTTAAGCGTAATAACAGCAACCATAAGGATTTTGTTGAACATGAAGAAAAACGTAAATCACTTTTGAAAATAGCTATCAATGCATTTAAAAAAAGAACTACCTTTAAAACACTTTACAGTGATATGGTTCAAAACGCTAAATATGGTAATGACTCACTTGAAAGGGCTTATGAAATGATTGGTAATAAACCTACCTTAGTTATTTTAGGAGCTAATGATATTGTCACGCCAACCAAAGCTTCTGTAGACTATCTAGCTAATAAAAGTGACAAGATTATTTTTAAGGTTATTGATGGTGTTGGTCATTCACCACACGACTCAGCGCCTAAACTTTTTTTTGATTATGTGCTAGAGTTTCTAGATAATTTAAAGAAACAAAGGTACTAA", "aa": "MFKKKKWPFFTFNFPGHGDNESTDTDQLKLNHFVDLVCDFIVQKKLNNVILIGHSMGGAVAVLVNKVIPLKIKALILVAPMNQTSFSVNKKRILDTFFKRNNSNHKDFVEHEEKRKSLLKIAINAFKKRTTFKTLYSDMVQNAKYGNDSLERAYEMIGNKPTLVILGANDIVTPTKASVDYLANKSDKIIFKVIDGVGHSPHDSAPKLFFDYVLEFLDNLKKQRY", "aa_hexdigest": "ed4b882aa75d04a05b4d39f28ccbc303", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q49418", "query_cov": 1.0, "subject_cov": 0.8395522388059702, "identity": 0.996, "score": 444.0, "evalue": 6.68e-157, "valid": true, "gene": "MenH", "product": "Putative esterase/lipase 2", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75311", "cog_id": "COG0596", "cog_category": "HR", "go_ids": [ "GO:0052689" ] }, "pscc": { "uniref50_id": "UniRef50_P75311", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75311" ], "product": "Putative esterase/lipase 2" }, "genes": [ "menH" ], "id": "KMADJIBOEG_754", "locus": "GCA000292445_00750" }, { "type": "cds", "sequence": "contig_1", "start": 410173, "stop": 410643, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGATGCTAATGAACAATACCAAGATTTACTCTCTGCTTTTGAAGAAACAAACAGTGAATTGGAGAAAGCCAAGCAAAGTTTAAGTGCATCTGATAGTGAAAATAATCAGTTAAAACAACAAATAAATTCACTTGAAAATGCTAAAAAAGAACTTCAATTTACTCCAGTTACCAGTGATGAACATCTTGATGAACTAGAAACATTAAAAATTGAAAAAGAACAACTTTTTTTAGAAAACCAAGCACTGCAAAATCAGTTGCAATATTTCAATGACATTAGTGCTAATCAAACTGAAGAGATCAAAGAAGCTAGTGATGAAGATAAACCAGTTGAAATAAAAAAACCACGAATTAAAAAACGTGATTTTGTTATCCAAAATAAGGATGATAAACTCGCTAAACTTTCTAAGAAAGAACGCATCCAAGCATATGCAGAACGTTTAGCTAAAATCAATGCTAACGAGTAA", "aa": "MNDANEQYQDLLSAFEETNSELEKAKQSLSASDSENNQLKQQINSLENAKKELQFTPVTSDEHLDELETLKIEKEQLFLENQALQNQLQYFNDISANQTEEIKEASDEDKPVEIKKPRIKKRDFVIQNKDDKLAKLSKKERIQAYAERLAKINANE", "aa_hexdigest": "97b4ac2e7bea49e75e88f8111ed3bb99", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18115.818199999998, "isoelectric_point": 4.790356254577637 }, "id": "KMADJIBOEG_755", "locus": "GCA000292445_00751" }, { "type": "cds", "sequence": "contig_1", "start": 411058, "stop": 412443, "strand": "-", "frame": 1, "gene": "yhgE", "product": "putative protein MG328", "db_xrefs": [ "COG:COG1511", "COG:S", "SO:0001217", "UniRef:UniRef50_Q49419", "UniRef:UniRef90_Q49419" ], "nt": "ATGGCAGTAGATAAGGAACTTGAAGTTAGTGATTTTGATAATGAGCTTGATGAAAAAACACTTTTAAAAGAATTAGTACAAAGAACTAATAATATCCTTTTTTCACCATCTAAAATTACTGCTATTCCCTTTGAAAGAAATTTATTGGAGAAAACATTTTTTGGTACTGTTGATGAAGCTGAGAAAGAAAAGTCAATAGTTTCTTTTTTTAATTGGATGATAGATTTAAAGGTTTTAGATAAAAAATGGGATAAGAATGTTTTAAATCATCATGCAAATCAACTTAAAACTAGAGAGGAAGAGCAACAGACAGTTGATCAAACTATGGCTTTTCAAGAAGTTGATGATCAATCAGTTTTAACCAAGGAAATTAAAACTGGTTTTCAGGAACTAAAACCATCAGTGATTACTGCTGAAGATGATAAGGATGAGATAAAACCAGAAGCTACTAAACAAGTATCTTTTGAAGAGTTGTTTAACCAACCTAGTGAAGAGATTAATGAAACAAAAAAACCAGAAGTTCAAATATTTTCAACTGATAAGGTTAAAGAACCTGAACAATTTGATGATTTCTATTCCATTGAAAATTTAACCAAAGCTATCAATCCAGTTCATAAAACGATCCAATATGATCAGAATGATGATCAACCTTTTGTTGTTAAAAGGATCTTAAAAGAGCAACATCCAACAAAAAAAGTTGATGAATTGGATGACTATAATAACAAAGAGCTTTTATTGGAAAATGCTGATTTAAAAAAACAAATCGATGATCTTAAAGAGAATAATAATGATCAAATATTTGATTTAGAACAGGAAATCGATGATCTTAAAAGGAGGTTATCTGAGGAAAAAAGTAAGCACTTACATACCAAGAAATTACAGGATGATCTTTTGCAAGAAAATAGGGATTTATATGAGCAACTGCAAAATAAACCAGTTGCTATTAATCCTTTAAGTGATGAAGTTAATGAAGAGTTGGAGAATTTAAAACAAGAAAAAGCTTTGCTTTCAGATCAATTAGATGCATTGAAAAATAAGAGCAGTAATGTTCAACAACAATTAGCTTTATTACCAGTTTTAAACAACCAAATAAACGAACTTCAAAACCAGTTACTAACAGCAAGAGAAGCAAACCAAAGATTGGATCTAGTTGAACAAGAAAATGATTTTTTGAAAAATGAACTGAAAAAACTCCATGACAACACATCTAATGATGAGAATGAAAAATATGATGATCTTTTAAATCAGTATGAACTACTTTTTGATGAGAATGAAACAAAATTTGATAAATTGCAAGTGAAACAACAAGCGCTAAACTTAGATTATCAAAAAACAATTTCAGCACTTAAACATGAAAATGATGTTTTATTAGATGAAATTGAGTGA", "aa": "MAVDKELEVSDFDNELDEKTLLKELVQRTNNILFSPSKITAIPFERNLLEKTFFGTVDEAEKEKSIVSFFNWMIDLKVLDKKWDKNVLNHHANQLKTREEEQQTVDQTMAFQEVDDQSVLTKEIKTGFQELKPSVITAEDDKDEIKPEATKQVSFEELFNQPSEEINETKKPEVQIFSTDKVKEPEQFDDFYSIENLTKAINPVHKTIQYDQNDDQPFVVKRILKEQHPTKKVDELDDYNNKELLLENADLKKQIDDLKENNNDQIFDLEQEIDDLKRRLSEEKSKHLHTKKLQDDLLQENRDLYEQLQNKPVAINPLSDEVNEELENLKQEKALLSDQLDALKNKSSNVQQQLALLPVLNNQINELQNQLLTAREANQRLDLVEQENDFLKNELKKLHDNTSNDENEKYDDLLNQYELLFDENETKFDKLQVKQQALNLDYQKTISALKHENDVLLDEIE", "aa_hexdigest": "0e2421413e313b62f8f70d5de25b2370", "start_type": "ATG", "rbs_motif": null, "genes": [ "yhgE" ], "truncated": "5-prime", "pseudogene": { "start": 410755, "stop": 412443, "observations": { "mutation": [ 411060 ], "stop": [ 411060 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49419", "query_cov": 1.0, "subject_cov": 0.6097883597883598, "identity": 0.993, "score": 811.0, "evalue": 2.31e-290, "gene_start": 1, "gene_end": 461, "reference_start": 1, "reference_end": 461, "reference_sequence": "MAVDKELEISDFDNELDEKTLLKELVQRTNNILFSPSKITAIPFERNLLEKTFFGTVDEAEKEKSIVSFFNWMIDLKVLDKKWDKNVLNHYANQLKTREEEQQTVDQTMAFQEVDDQSVLTKEIKTGFQELKPSVITAEDDKDEIKPEATKQVSFEELFNQPSEEINETKKPEVQIFSTDKVKEPEQFDDFYSIENLTKAINPVHKTIQYDQNDDQPFVVKRILKEQHPTKKVDELDDYNNKELLLENADLKKQIDDLKENNNDQIFDLEQEIDDLKRRLSEEKSKHLHTKKLQDDLLQENRDLYEQLQNKPVAINPLSDEVNEELENLKQEKALLSDQLDALKNKSSNVQQQLALLPVLNNQINELQNQLLTAREANQRLDLVEQENDFLKNELKKLHDNTSNDENEKYDDLLNQYELLFDENETKFDKLQVKQQALNLDYQKTISALKHENDVLLDEIEWTRSKDNDFNNTKNSFEEQKKALDEKLNGLTIQNQQLQDKIAELEEWNEEKSNLNTNQLVNLQQQLKDSQNLFNVAQDKLATLEEVNLALNEKINDLEDELSGSENSNNLLAKLQADHEILQESYGKLKTDFEKLKKNKLNDANEQYQDLLSAFEETNSELEKAKQSLSASDSENNQLKQQINSLENAKKELQFTPVTSDEHLDELETLKIEKEQLFLENQALQNQLQYFNDISANQTEEIKEASDEDKPVEIKKPRIKKRDFVIQNKDDKLAKLSKKERIQAYAERLAKINANE" }, "paralog": false, "identity": 0.9893238434163701, "score": 1050.0, "evalue": 0.0, "description": "Internal stop codon at 411060. Nonsense mutation around 411060.", "psc": { "uniref90_id": "UniRef90_Q49419", "gene": "YhgE", "product": "Uncharacterized protein MG328", "uniref50_id": "UniRef50_Q49419", "cog_id": "COG1511", "cog_category": "S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49419", "UniRef:UniRef50_Q49419", "COG:COG1511", "COG:S" ] }, "pscc": { "uniref50_id": "UniRef50_Q49419", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49419" ], "product": "Uncharacterized protein MG328" } }, "id": "KMADJIBOEG_756", "locus": "GCA000292445_00752" }, { "type": "cds", "sequence": "contig_1", "start": 412482, "stop": 413828, "strand": "-", "frame": 2, "gene": "der", "product": "ribosome biogenesis GTPase Der", "db_xrefs": [ "COG:COG1160", "COG:J", "EC:3.6.5.-", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022", "KEGG:K03977", "RefSeq:WP_014894293.1", "SO:0001217", "UniParc:UPI00027B3F58", "UniRef:UniRef100_UPI00027B3F58", "UniRef:UniRef50_P75309", "UniRef:UniRef90_P47571" ], "nt": "GTGTTTACTGTTGCAATAATAGGTCGTACAAACGTTGGTAAATCAACTTTATTTAATCGCTTAATTCAAAAGCCAATGGCTATTGTTTCAGATACTCCTAACACAACAAGAGATAGGATCTTTGGAATTGGTGAATGGTTAAAAAGAAAGATAGCTTTTATTGACACAGGTGGATTAATTGCAAAACAAACTCCATTACAACAACTAATTGCATTACAAGCACAAGCAGCACTTAGTCAAGCCAAAGCTATTATTTTTTTAGTGTCACTACAAGAACAACTAAATAGCGATGATTTTTATGTAGCTAAGGTTCTTAAAAAAAATAAGGATAAACCAGTAATACTAGTGGTAAATAAAGCTGAAAATTTTAACCCTAAAACTGCTGAAGAAACATTAAAAGATTATTACAGCTTAGGGTTTGGAAGACCAGTTGTTATTAGTGCTGCTCATGGAATTGGAATTGGTGATTTAATGGATCTCTTAGTTAAACAAAATCAATTACTCCCTAATGAAAATAATGATGATTTAGCAAAAATAAGGTTTTGTGTAATTGGCAAACCAAATGTTGGTAAAAGCTCACTTATTAATCAACTTGTAAAACAAAATCGTGTTTTAGTTTCAAATGAGAGTGGTACAACTAGAGATGCTATTGATGTTCCTTTAAAAGTTAATGGTGAAAAATTTCTTTTGATTGATACTGCTGGTATTAAAAGAAAAGGCAAAATTAACATGGGAATTGAAACCGCATCTTACATCAAAACAAAGCTAGCTATTGCCCGTAGTAACGTTATCCTTTTGATGGTAGATGGATCAAAACCTATTAGTGAACAAGATGAAGTGATTGGTGGATTGGCACAAGCTGCACTTATCCCTGTTATTATTCTTGTGAATAAATGGGATCTAATTTTAAAAAACAATAACACCACAAATGCATATAAAAAAATGCTGAAATTGCATTTTAAACACCTCGATTTTGCGCCTGTTTTGTTTATTAGTGTTTTGAAAAATCAGCGCTTAAATACTATTTTTGAACAACTTAAAATTATTCAAAGCCAACTTGAAACTAAGGTTGCAACACCACTTTTAAATGATGTTATTCAGCAAGCACAACTTTACAATCAACCTCCACTTTTTAAAGGCAAGCGTTTGCAGATAACTTACGCAGTACAAACCAAAAGTCAAATTCCCCATTTTGTTTTATTTTGCAATGACCCTAAGTATCTTCATTTTTCTTATGCACGTTTTTTAGAGAATAAGATTAGAGAAAACTTTGGTTTTAATTCTGTACCTATTAGCCTTTACTTTAAATCAAAAAACGCACGGATAAGAACAAAGCCAGAAGTTTAA", "aa": "MFTVAIIGRTNVGKSTLFNRLIQKPMAIVSDTPNTTRDRIFGIGEWLKRKIAFIDTGGLIAKQTPLQQLIALQAQAALSQAKAIIFLVSLQEQLNSDDFYVAKVLKKNKDKPVILVVNKAENFNPKTAEETLKDYYSLGFGRPVVISAAHGIGIGDLMDLLVKQNQLLPNENNDDLAKIRFCVIGKPNVGKSSLINQLVKQNRVLVSNESGTTRDAIDVPLKVNGEKFLLIDTAGIKRKGKINMGIETASYIKTKLAIARSNVILLMVDGSKPISEQDEVIGGLAQAALIPVIILVNKWDLILKNNNTTNAYKKMLKLHFKHLDFAPVLFISVLKNQRLNTIFEQLKIIQSQLETKVATPLLNDVIQQAQLYNQPPLFKGKRLQITYAVQTKSQIPHFVLFCNDPKYLHFSYARFLENKIRENFGFNSVPISLYFKSKNARIRTKPEV", "aa_hexdigest": "dd0086670b00f48acc890c4a7084fa7b", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3F58", "ncbi_nrp_id": "WP_014894293.1", "uniref100_id": "UniRef100_UPI00027B3F58", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3F58", "RefSeq:WP_014894293.1", "UniRef:UniRef100_UPI00027B3F58" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3F58", "uniref90_id": "UniRef90_P47571" }, "psc": { "uniref90_id": "UniRef90_P47571", "gene": "der", "product": "ribosome biogenesis GTPase Der", "ec_ids": [ "3.6.5.-" ], "uniref50_id": "UniRef50_P75309", "cog_id": "COG1160", "cog_category": "J", "kegg_orthology_id": "K03977", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_P75309", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75309" ], "product": "GTPase Der" }, "genes": [ "der" ], "id": "KMADJIBOEG_757", "locus": "GCA000292445_00753" }, { "type": "cds", "sequence": "contig_1", "start": 413832, "stop": 414278, "strand": "-", "frame": 2, "gene": "cmk", "product": "(d)CMP kinase", "db_xrefs": [ "COG:COG0283", "COG:F", "EC:2.7.4.25", "GO:0004127", "GO:0005524", "GO:0005829", "GO:0006220", "GO:0015949", "GO:0036430", "GO:0036431", "SO:0001217", "UniRef:UniRef50_P47572", "UniRef:UniRef90_P47572" ], "nt": "GTGTATTATAACAATGCTGATATTACAACAGTTATTACAACCCAATCAGTTGCTAACATTGCTAGTAAAATAGCTGTTGATCCTAACATTAGAAAAATTGCAGTTATTAAACAACAGAAACTAGCAGAAAATAAAAACATAGTGATGGATGGTAGAGACATAGGAACAGTAGTTTTAAAAAATGCTCAATTGAAGTTTTTTTTAGATGCTAAAGTTGAAATTAGAGCGCAGCGAAGATTACAAGATATGGGAATTTCTCTATCAAATGAAAAAAAACTAAAGGAACTAATTCAAGAATTAAAGCAACGTGATCAAATTGATAGTTCTAGAACTGCAGACCCATTAAAAAAAGCCCAGGACGCTATTTATCTTGACACTTCTGAACTAAGTTTTGATGCAGTAGTAAAACAAACCCTCAAAGAAGCAAAGAAGGTTTTTAAACTTTAA", "aa": "MYYNNADITTVITTQSVANIASKIAVDPNIRKIAVIKQQKLAENKNIVMDGRDIGTVVLKNAQLKFFLDAKVEIRAQRRLQDMGISLSNEKKLKELIQELKQRDQIDSSRTADPLKKAQDAIYLDTSELSFDAVVKQTLKEAKKVFKL", "aa_hexdigest": "d73d108ff95f14321377c73a3df6cde7", "start_type": "GTG", "rbs_motif": null, "genes": [ "cmk" ], "truncated": "3-prime", "pseudogene": { "start": 413832, "stop": 414485, "observations": { "mutation": [ 414299, 414479 ], "stop": [ 414299, 414479 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47572", "query_cov": 1.0, "subject_cov": 0.6820276497695853, "identity": 0.993, "score": 269.0, "evalue": 7.1e-90, "gene_start": 1, "gene_end": 148, "reference_start": 70, "reference_end": 217, "reference_sequence": "MNWQIAIDGPSSSGKSSVAKKIAEELDFFYFSSGKMYRAFAYVMQVNRLNIDLFLKIINQINWRFEKDAVYYNNADITTVITTQSVANIASKIAVDPNIRKIAVIKQQKLAENKNIVMDGRDIGTVVLKNAQLKFFLDAKVEIRAQRRLQDMGISLSNEKKLKELIQELKQRDQIDSSRTADPLKKAQDAIYLDTSELSFDAVVKQTLKEAKKVFKL" }, "paralog": false, "identity": 0.9907834101382489, "score": 405.0, "evalue": 3.9e-146, "description": "Internal stop codon at 414299, 414479. Nonsense mutation around 414299, 414479.", "psc": { "uniref90_id": "UniRef90_P47572", "gene": "cmk", "product": "(d)CMP kinase", "ec_ids": [ "2.7.4.25" ], "uniref50_id": "UniRef50_P47572", "cog_id": "COG0283", "cog_category": "F", "go_ids": [ "GO:0004127", "GO:0005524", "GO:0005829", "GO:0006220", "GO:0015949", "GO:0036430", "GO:0036431" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47572", "EC:2.7.4.25", "UniRef:UniRef50_P47572", "COG:COG0283", "COG:F", "GO:0004127", "GO:0005524", "GO:0005829", "GO:0006220", "GO:0015949", "GO:0036430", "GO:0036431" ] }, "pscc": { "uniref50_id": "UniRef50_P47572", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47572" ], "product": "Cytidylate kinase" } }, "id": "KMADJIBOEG_758", "locus": "GCA000292445_00754" }, { "type": "cds", "sequence": "contig_1", "start": 414543, "stop": 414752, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557EBC", "UniRef:UniRef90_UPI0000557EBC" ], "nt": "ATGCTAAACAGCATGAAACTTATTAAAGTTATCACTAAGTTCTTAAAAATAGCCTTATTAGCATTAAACAAATTAGGTTCGATGTTATCAATGCTTTTTTTAAAAGCAAAACTTTCAGTTGAAAAAACTGTTAGTATCTTGTTGTATTCTTTTAAATCACTAGTTAAATTGCTTGTTTTTTTTAATCATTGTTTCTCATTAGCTTCATAA", "aa": "MLNSMKLIKVITKFLKIALLALNKLGSMLSMLFLKAKLSVEKTVSILLYSFKSLVKLLVFFNHCFSLAS", "aa_hexdigest": "d66a2511a7f38532eb179d83cb5663cc", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0000557EBC", "query_cov": 0.8260869565217391, "subject_cov": 1.0, "identity": 1.0, "score": 95.1, "evalue": 1.43e-24, "valid": true, "uniref50_id": "UniRef50_UPI0000557EBC" }, "pscc": { "uniref50_id": "UniRef50_UPI0000557EBC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557EBC" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7781.673299999997, "isoelectric_point": 10.252229881286617 }, "id": "KMADJIBOEG_759", "locus": "GCA000292445_00755" }, { "type": "cds", "sequence": "contig_1", "start": 414800, "stop": 414997, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B4188E", "UniRef:UniRef90_UPI0001B4188E" ], "nt": "TTGATTAATTACCATCCTTTTAGTGAATTTAATAAAAATCCAGTTAAACATCACACTGAACCAAATAAACTGTTTAAAACACTTCAGGAATTAATAGTTGATTTAAAAAATACTGACTTTAAATTATTAGAAGAAAAAGTTGATAGGATGTGGTTAAACGCAGCATATAACCAAACTTCTAGCGGTTATGAAAGCTGA", "aa": "MINYHPFSEFNKNPVKHHTEPNKLFKTLQELIVDLKNTDFKLLEEKVDRMWLNAAYNQTSSGYES", "aa_hexdigest": "1918e9d197e5c33827961012463bbd57", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 414702, "stop": 415132, "observations": { "deletion": [ 414730 ], "mutation": [ 414802, 415033 ], "stop": [ 414802, 415033 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001B4188E", "query_cov": 1.0, "subject_cov": 0.45454545454545453, "identity": 0.985, "score": 135.0, "evalue": 2e-39, "gene_start": 1, "gene_end": 65, "reference_start": 46, "reference_end": 110, "reference_sequence": "MGRVEKFRFYRQSFDNNKIVKKALINAQKNTESWKKQLNKINQKILINYHPFSEFNKNPVKHHTEPNKLFKTLQELIVDLKNTDFKLLEEKVDRMWLNAAYNQTSSGYESWISDDKGIEKINHLSKFYEANEKQWLKKNKQFN" }, "paralog": false, "identity": 0.972027972027972, "score": 275.0, "evalue": 2.42e-97, "description": "Internal stop codon at 414802, 415033. Frameshift due to deletion around 414730. Nonsense mutation around 414802, 415033.", "psc": { "uniref90_id": "UniRef90_UPI0001B4188E", "uniref50_id": "UniRef50_UPI0001B4188E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001B4188E", "UniRef:UniRef50_UPI0001B4188E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001B4188E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B4188E" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7743.653400000003, "isoelectric_point": 6.254642295837402 }, "id": "KMADJIBOEG_760", "locus": "GCA000292445_00756" }, { "type": "cds", "sequence": "contig_1", "start": 415031, "stop": 415132, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAGAGTAGAAAAATTTCGCTTTTATAGACAAAGTTTTGATAACAATAAGATAGTTAAAAAAGCTTTAATTAACGCTCAGAAAAACACTGAAAGCTGA", "aa": "MGRVEKFRFYRQSFDNNKIVKKALINAQKNTES", "aa_hexdigest": "218d173b86910ef8438da546ab36ae66", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { 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"ATGCCACGTAAGCATCTAATTGCTAATCAAACTAATAAAAAACAACAAACAAGTGCAAAACAACTTCAAAAACTAGCAAAAAGAATAGCTTCAGCTGTTAAAAAAGGTGGAACTAATATCCAGTCAAATCCACATCTAAAAGTTGCAGTTGATCTTGCTTTAGCTAAGGGTCTAAGCATGGATTCAATTAAAAGAAATATCCATGGTAGTGAAAAAGATACAACTAAAATTAGCGAGTTTTGTTATGAGATTTTTGGACCAAATGGTGTTGGAATTATTGTGTTTGGACTAACTGATAATCCTAACCGTTTACTCAGTAGTTTAAACGGTTATTTAGCTAAACTAAAAGGACAATTAGCCAAGCCAAATAGCGTCAAGATTAATTTTCAAGAAGAAGGGATTATCTTTGTTAATAAAAATAACTATTTGAAAGATGATCTAATTGAATTATTAATTTTGGACAATATTAACTTAATTGATGTTGATTATGATGAAGAGTGTTTTGAAATTAGCTTGCATTCAAATAGTTATTTTCATGCAAAGGAACTGTTGAAAAAAAACAATTTTTCAATAGTAGATAGTGAAATTAAATTGGTACCTCTTTTAACTGTTGATTTAGATAGAAATCAGCAAACTTTATTATCACGTTTTCTCAATGCTTGTGAGGAAGATGATGACATTCAGTTTGTTGTTCATAATGCCAACCCATGGGAAGAGTAG", "aa": "MPRKHLIANQTNKKQQTSAKQLQKLAKRIASAVKKGGTNIQSNPHLKVAVDLALAKGLSMDSIKRNIHGSEKDTTKISEFCYEIFGPNGVGIIVFGLTDNPNRLLSSLNGYLAKLKGQLAKPNSVKINFQEEGIIFVNKNNYLKDDLIELLILDNINLIDVDYDEECFEISLHSNSYFHAKELLKKNNFSIVDSEIKLVPLLTVDLDRNQQTLLSRFLNACEEDDDIQFVVHNANPWEE", "aa_hexdigest": "8eb09ed5c376415173eb966cf3d8aec2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013963B", "ncbi_nrp_id": "WP_009885985.1", "uniref100_id": "UniRef100_P47574", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013963B", "RefSeq:WP_009885985.1", "UniRef:UniRef100_P47574" ] }, "ips": { "uniref100_id": "UniRef100_P47574", "uniref90_id": "UniRef90_P47574" }, "psc": { "uniref90_id": "UniRef90_P47574", "gene": "TACO1", "product": "Probable transcriptional regulatory protein MG332", "uniref50_id": "UniRef50_P47574", "cog_id": "COG0217", "cog_category": "KJ", "go_ids": [ "GO:0005829", "GO:0003677", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_P47574", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47574" ], "product": "Probable transcriptional regulatory protein MG332" }, "genes": [ "tACO1" ], "id": "KMADJIBOEG_762", "locus": "GCA000292445_00758" }, { "type": "cds", "sequence": "contig_1", "start": 415846, "stop": 415983, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGGTTTTGCAGGTGTTACACATTTTGAGAGTGTTTTAATTGATGACACTAAAATTCTTTACAAAGATAAAAACAAGCAAGAGGTAGTTGATCTGTTTGCACACAAAGTTGACCAAGTTGCTAATAATTTTTAA", "aa": "MFGFAGVTHFESVLIDDTKILYKDKNKQEVVDLFAHKVDQVANNF", "aa_hexdigest": "82e4ebb11438c9b9f04963f8376f769a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5172.820200000002, "isoelectric_point": 5.393304252624512 }, "id": "KMADJIBOEG_763", "locus": "GCA000292445_00759" }, { "type": "cds", "sequence": "contig_1", "start": 416020, "stop": 416184, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02525.22" ], "nt": "ATGACCAACTTTAATTATCCTGCTAGTTTAAAAAACTTTATTGATCATGTCTTTGTAGCTAATAAAACCTTTCAAGACAAATATGTTACTAAAGGTGCTTCTAAGGGATTACTTACTAATCTTAAGGTAGTTGTTTTAGCGTCACAAGGTGCACCATTAGGATGA", "aa": "MTNFNYPASLKNFIDHVFVANKTFQDKYVTKGASKGLLTNLKVVVLASQGAPLG", "aa_hexdigest": "cd4115273f6ad6fa7b62f103ec3e3d2f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02525.22", "name": "Flavodoxin_2", "length": 52, "aa_cov": 0.9629629629629629, "hmm_cov": 0.9807692307692307, "evalue": 3.455565406513069e-13, "score": 44.84715270996094, "start": 1, "stop": 52 } ], "seq_stats": { "molecular_weight": 5857.734000000002, "isoelectric_point": 9.778580665588379 }, "id": "KMADJIBOEG_764", "locus": "GCA000292445_00760" }, { "type": "cds", "sequence": "contig_1", "start": 416317, "stop": 416439, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAGTTATTATTGTTGATGCTTCTGTAACCCCTTCTGGTTCTTACACCCACCTTTTATTAGATCGTTTCCTTCAAACTTATAAAAAACAAAATAGTAGTGTTGAATTTATTGATTGA", "aa": "MKKVIIVDASVTPSGSYTHLLLDRFLQTYKKQNSSVEFID", "aa_hexdigest": "8f3084ab4a5b37a563b88e61619cbfe8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4573.226500000001, "isoelectric_point": 8.12071170806885 }, "id": "KMADJIBOEG_765", "locus": "GCA000292445_00761" }, { "type": "cds", "sequence": "contig_1", "start": 417304, "stop": 417537, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGTTATTTAACTCCTTTTTTGAAACTAAAAAACTGCCATTTAAAACTGTTTTAATCCACGGTTTAGTACGCGATGAACAAAATCGTAAGATGTCAAAATCACTGAATAATGGCATTGATCCTGTTGATCTTATTAGAAATTATGGAGCGGATGCAGTGCGCTTATTTTTGTGTTCAAATCACACTCCAGGAGATGATCTAATTTTCAGCGAACAAAAAATAAAAAGTGCATGA", "aa": "MLFNSFFETKKLPFKTVLIHGLVRDEQNRKMSKSLNNGIDPVDLIRNYGADAVRLFLCSNHTPGDDLIFSEQKIKSA", "aa_hexdigest": "4ca030f6146eb7eee758fc78ef2bb7eb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001D01CCB6", "query_cov": 0.974025974025974, "subject_cov": 0.8620689655172413, "identity": 0.547, "score": 90.1, "evalue": 4.21e-22, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A3B0PEY5" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 61, "aa_cov": 0.7922077922077922, "hmm_cov": 0.9836065573770492, "evalue": 3.430212427236888e-21, "score": 70.54769897460938, "start": 7, "stop": 67 } ], "seq_stats": { "molecular_weight": 8784.024499999996, "isoelectric_point": 8.889432716369633 }, "id": "KMADJIBOEG_766", "locus": "GCA000292445_00762" }, { "type": "cds", "sequence": "contig_1", "start": 418009, "stop": 418170, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATGTACTCCTGCACACGACTTTAATGACTATGAAATCAACACTAAATATAAATTTGATTTTCTAAGCTGCATTGACAGTAACGGTATTCTCAATCAAAATGCAAGTAAATTTCAAGGCCTTAGTGTTTTACAAGCAAGAAATAAAATTGTTAAATGA", "aa": "MKCTPAHDFNDYEINTKYKFDFLSCIDSNGILNQNASKFQGLSVLQARNKIVK", "aa_hexdigest": "c4bd564551a58b5abec6317514ccb895", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6069.855600000002, "isoelectric_point": 8.685003471374515 }, "id": "KMADJIBOEG_767", "locus": "GCA000292445_00763" }, { "type": "cds", "sequence": "contig_1", "start": 418258, "stop": 418446, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATACTGCTATATCAAATATTGAAGTTATCAATAAACCTGTTAATCAACATCTTCATTATGTTGTTTATAAACTAGCGAATGATAGTAAACAAGAACTAATAGTTGCAACAACAAGACCAGAAACTATCTTTGCTGATGTTTGTCTATTGGTAAACCCAAATGATAAGCGCTATACTAATTTCTGA", "aa": "MNTAISNIEVINKPVNQHLHYVVYKLANDSKQELIVATTRPETIFADVCLLVNPNDKRYTNF", "aa_hexdigest": "0ef6a5a3782e764aa4c58670a00315b4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7118.068300000002, "isoelectric_point": 6.701567268371583 }, "id": "KMADJIBOEG_768", "locus": "GCA000292445_00764" }, { "type": "cds", "sequence": "contig_1", "start": 418979, "stop": 419554, "strand": "-", "frame": 3, "gene": "yihA", "product": "ribosome biogenesis GTP-binding protein YihA/YsxC", "db_xrefs": [ "COG:COG0218", "COG:D", "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0042254", "GO:0043022", "GO:0046872", "RefSeq:WP_014894297.1", "SO:0001217", "UniParc:UPI00027B3D34", "UniRef:UniRef100_UPI00027B3D34", "UniRef:UniRef50_P47577", "UniRef:UniRef90_P47577" ], "nt": "ATGGATGCACATTTTTTGAAGAGTGCAAGTGATTTAAAAGATTGTCCTCAAGATAATATCCCAGAGATTTGTTTCATGGGAAGAAGTAATGTTGGTAAATCAAGTTTGATTAATGCTTTTTTTAAAAAGAAACTGGCTAAAACTTCAGCAACACCAGGTCGAACACAATTACTAAATTACTTTGAATATAAAGACAAAAGATTTGTTGATTTACCAGGTTATGGCTTTGCTAAAATAAGCAAAAATAAGAAAGATTTTATTACTAATTTACTTACCCAATTTTTAAATTTTCGTAGTAATCTAGTTGGTGTTGTTTTGATAGTAGATAGTGGAGTTGTTACTGTTCAAGATCAGGAAGTGGTTAAAATTATTTTGCAAACAGGATTGAACTTTTTGATTGTTGCTAATAAATTTGACAAACTAAATCAAAGTGAAAGATATTTTTCGCTTAAGAATATTGCAAATTTTTTAAAAGTTAATTTTGATAAATGTGTTTTTGCAAGTACAAAAACACATCATAATCTTGCTTTAGTTCACAAAAAAATTTTCGAATTATTCGTTGAAGATGAAAGATAA", "aa": "MDAHFLKSASDLKDCPQDNIPEICFMGRSNVGKSSLINAFFKKKLAKTSATPGRTQLLNYFEYKDKRFVDLPGYGFAKISKNKKDFITNLLTQFLNFRSNLVGVVLIVDSGVVTVQDQEVVKIILQTGLNFLIVANKFDKLNQSERYFSLKNIANFLKVNFDKCVFASTKTHHNLALVHKKIFELFVEDER", "aa_hexdigest": "b038ecfc1be45f7b55bbe3ccc377b14d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3D34", "ncbi_nrp_id": "WP_014894297.1", "uniref100_id": "UniRef100_UPI00027B3D34", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3D34", "RefSeq:WP_014894297.1", "UniRef:UniRef100_UPI00027B3D34" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3D34", "uniref90_id": "UniRef90_P47577" }, "psc": { "uniref90_id": "UniRef90_P47577", "gene": "yihA", "product": "ribosome biogenesis GTP-binding protein YihA/YsxC", "uniref50_id": "UniRef50_P47577", "cog_id": "COG0218", "cog_category": "D", "go_ids": [ "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0042254", "GO:0043022", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47577", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47577" ], "product": "Probable GTP-binding protein EngB" }, "genes": [ "yihA" ], "id": "KMADJIBOEG_769", "locus": "GCA000292445_00765" }, { "type": "cds", "sequence": "contig_1", "start": 419562, "stop": 419783, "strand": "-", "frame": 2, "gene": "yneF", "product": "UPF0154 MG335-1-like protein", "db_xrefs": [ "COG:COG3763", "COG:S", "GO:0016020", "KEGG:K09976", "RefSeq:WP_009885966.1", "SO:0001217", "UniParc:UPI0000139652", "UniRef:UniRef100_Q49310", "UniRef:UniRef50_Q9EXD7", "UniRef:UniRef90_Q9EXD7" ], "nt": "ATGAATGATCTAGCACTTGCATTAGGTTTAGGTATCCCTCTTTCGCTTTTGGTGGGAATGATTCTTGGTTATTTTATTTCTATAAAAATCTTTAAAAAACAGATGCGTGACAATCCACCTATTACTGAAAATCAAATTAAAGCAATGTATGCAAAAATGGGTAGAAAATTATCTGAAACCCAGGTAAAAGAGATAATGCGCTCCATTAAAAACCAAAAATAA", "aa": "MNDLALALGLGIPLSLLVGMILGYFISIKIFKKQMRDNPPITENQIKAMYAKMGRKLSETQVKEIMRSIKNQK", "aa_hexdigest": "4ea5b5ae143c256ba0c296af9bf55d2d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139652", "ncbi_nrp_id": "WP_009885966.1", "uniref100_id": "UniRef100_Q49310", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139652", "RefSeq:WP_009885966.1", "UniRef:UniRef100_Q49310" ] }, "ips": { "uniref100_id": "UniRef100_Q49310", "uniref90_id": "UniRef90_Q9EXD7" }, "psc": { "uniref90_id": "UniRef90_Q9EXD7", "gene": "YneF", "product": "UPF0154 protein MG335.1 homolog", "uniref50_id": "UniRef50_Q9EXD7", "cog_id": "COG3763", "cog_category": "S", "kegg_orthology_id": "K09976", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_Q9EXD7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9EXD7" ], "product": "UPF0154 protein MG335.1 homolog" }, "genes": [ "yneF" ], "id": "KMADJIBOEG_770", "locus": "GCA000292445_00766" }, { "type": "cds", "sequence": "contig_1", "start": 419871, "stop": 420353, "strand": "+", "frame": 3, "gene": "wcaA", "product": "Processive diacylglycerol beta-glycosyltransferase", "db_xrefs": [ "COG:COG0463", "COG:M", "EC:2.4.1.-", "GO:0005886", "GO:0006071", "GO:0009246", "GO:0016758", "GO:0046467", "GO:0046509", "KEGG:K19004", "SO:0001217", "UniRef:UniRef50_Q9ZB73", "UniRef:UniRef90_Q9ZB73" ], "nt": "TTGTTACAAATCAAAACCTTTTTTAAAACGTTTTTTAATTCACTTTTAAAGCAAGATCTTAATCAAGCTAAAATTATTTTTTTCAATGACAATGTTGCTGATGAAACCTATGAAGTTTTGCAAAAATTCAAAAAAGAACACAATAACTTAGCAATTGAAGTCTATTGTGACAAACAGAATGAAGGTATTGGAAAAGTGCGTGATAAGCTAGTGAATCTAGTAACAACACCTTATTTTTATTTTATCGATCCTGATGATTGTTTTAACAACAAAAATGTCATAAAAGAGATTGTTGAATCAATTAAAAAAGAAGATTTTGATCTTGGTGTATTAAAAAGTATGGTCTATTTATGCTTCTTAAAACATGATTTCATTATTAAATTTTTGCCTTTAAAAGGTATTTTTCAAGGCAGAGTAAAATTAATTAATAATAACAATGTTAATAAATTAAATTACATCAAAAATAATGATCAATATATTTGA", "aa": "MLQIKTFFKTFFNSLLKQDLNQAKIIFFNDNVADETYEVLQKFKKEHNNLAIEVYCDKQNEGIGKVRDKLVNLVTTPYFYFIDPDDCFNNKNVIKEIVESIKKEDFDLGVLKSMVYLCFLKHDFIIKFLPLKGIFQGRVKLINNNNVNKLNYIKNNDQYI", "aa_hexdigest": "01a7f9a82d07e1dec99a75575a03ed49", "start_type": "TTG", "rbs_motif": null, "genes": [ "wcaA" ], "truncated": "3-prime", "pseudogene": { "start": 419842, "stop": 420656, "observations": { "deletion": [ 419902 ], "stop": [ 420352, 420433, 420646 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9ZB73", "query_cov": 0.975, "subject_cov": 0.4574780058651026, "identity": 0.981, "score": 308.0, "evalue": 3.11e-103, "gene_start": 5, "gene_end": 160, "reference_start": 15, "reference_end": 170, "reference_sequence": "MDKLVSILVPCYKSKPFLKRFFNSLLKQDLNQAKIIFFNDNVADETYEVLQKFKKEHNNLAIEVYCDKQNEGIGKVRDKLVNLVTTPYFYFIDPDDCFNNKNVIKEIVESIKKEDFDLGVLKSMVYLCFLKHDFIIKFLPLKGIFQGRVKLINNNNVNKLNYIKNNDQYIWNIVINTDFFRKLNLTFESRLFEDIPIWYPMFFSSQKIVFIDVIGTNYFIRNDSLSTTISAPRYLNLIQCYEKLYVNLSQNGSLASFIDPNHKIEARFWRRQMFVWFALFSFEYFKKNFSESKKILEKLFVFLEKNGVYERVFQTKNQGIYYIWVQRLKYFKHVLESKSDN" }, "paralog": false, "identity": 0.988929889298893, "score": 532.0, "evalue": 3.32e-194, "description": "Internal stop codon at 420352, 420433, 420646. Frameshift due to deletion around 419902.", "psc": { "uniref90_id": "UniRef90_Q9ZB73", "gene": "WcaA", "product": "Processive diacylglycerol beta-glycosyltransferase", "ec_ids": [ "2.4.1.-" ], "uniref50_id": "UniRef50_Q9ZB73", "cog_id": "COG0463", "cog_category": "M", "kegg_orthology_id": "K19004", "go_ids": [ "GO:0005886", "GO:0046509", "GO:0016758", "GO:0009246", "GO:0006071", "GO:0046467" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9ZB73", "EC:2.4.1.-", "UniRef:UniRef50_Q9ZB73", "COG:COG0463", "COG:M", "KEGG:K19004", "GO:0005886", "GO:0046509", "GO:0016758", "GO:0009246", "GO:0006071", "GO:0046467" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZB73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZB73" ], "product": "Processive diacylglycerol beta-glycosyltransferase" } }, "id": "KMADJIBOEG_771", "locus": "GCA000292445_00767" }, { "type": "cds", "sequence": "contig_1", "start": 420441, "stop": 420647, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTTTCATCACAAAAAATTGTTTTTATTGATGTGATAGGAACAAATTATTTTATTCGTAATGATAGTTTATCAACTACTATTAGTGCTCCACGCTACTTAAATTTAATCCAATGTTATGAAAAGCTATATGTAAATCTCAGCCAAAATGGTTCTCTTGCAAGTTTTATTGATCCAAATCATAAGATTGAAGCTAGGTTTTGA", "aa": "MFFSSQKIVFIDVIGTNYFIRNDSLSTTISAPRYLNLIQCYEKLYVNLSQNGSLASFIDPNHKIEARF", "aa_hexdigest": "2301ad5b52bd201c850b9dca25639653", "start_type": "ATG", "rbs_motif": "ATATA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7853.892699999998, "isoelectric_point": 7.8923646926879885 }, "id": "KMADJIBOEG_772", "locus": "GCA000292445_00768" }, { "type": "cds", "sequence": "contig_1", "start": 420975, "stop": 421358, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00266.25" ], "nt": "ATGTCTGCAATTAAATTTAATCCTAGTTCATTCAGAAAAAACTTTAAATGGTTTGAAAATAACAAAAATTGGATTAATTTTGATAATGCTGCTACTTCCATTGCACTTGATGTTGTGGCTGAAGCAAGCAAAGAATATTACCAGTATTTTTGTGTCAATCCTCATAACAAAAATCCTGAAATTAACCAAAAACTTATTGCTATTATTGAAGAAACAAGAGATTTATTAGCAAAATTTTTCAATGCTAAAAAAAATGAAATAATTTTTACAAGTTCTGCAACTGAATCGCTTAACTTATTCGCCTTTGGATTAAGCTCTTTAGTAAAAAGTAATGATGAAATCATTCTCAAAGAAGATGAACATGCTGCTAATGTTTTTCCCTGA", "aa": "MSAIKFNPSSFRKNFKWFENNKNWINFDNAATSIALDVVAEASKEYYQYFCVNPHNKNPEINQKLIAIIEETRDLLAKFFNAKKNEIIFTSSATESLNLFAFGLSSLVKSNDEIILKEDEHAANVFP", "aa_hexdigest": "d2254112d2d3c44a17244c1b4c4922ba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00266.25", "name": "Aminotran_5", "length": 104, "aa_cov": 0.8110236220472441, "hmm_cov": 1.0, "evalue": 3.575062467316446e-27, "score": 90.3916015625, "start": 25, "stop": 127 } ], "seq_stats": { "molecular_weight": 14573.285499999982, "isoelectric_point": 5.6869913101196286 }, "id": "KMADJIBOEG_773", "locus": "GCA000292445_00769" }, { "type": "cds", "sequence": "contig_1", "start": 421746, "stop": 422201, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00266.25" ], "nt": "TTGGATAATAACTTTAAGATAATTTTTAAAGAAGGTCCTTCCAAATTTGAAGCTGGAACGCTAAATATTATGGCTATTTATGCTTTGAATAAACAGTTAAAATTCATGCAAAAAGAATTTAATTTCAGTGAAATGGTGTTTTACAGCAAACAATTAAAAAATTTAGCTTATCAACTGCTAAGTCAAAATCCTAATATCGTTTTAGCTAATCATGATCAAGATGTTCCTATCTTTGCTTTTAAGCATAAATATATTAATTCTGCAGATCTAGCAACTTTTTTAAACATTAAAAAAATAATTGTTAGACAAGGATCCATCTGTGTTGGTAAATTTAAAAATAAAGAGAGTTTTTTACGTGTTTCTCTACTCCATTACAACACAAAAGAGGAATTACTTTATTTAGAAAAATTATTAAAAACTAGTAAGAATTCCATTATTAATGAACTAATATATTAG", "aa": "MDNNFKIIFKEGPSKFEAGTLNIMAIYALNKQLKFMQKEFNFSEMVFYSKQLKNLAYQLLSQNPNIVLANHDQDVPIFAFKHKYINSADLATFLNIKKIIVRQGSICVGKFKNKESFLRVSLLHYNTKEELLYLEKLLKTSKNSIINELIY", "aa_hexdigest": "97b774aef007d732051569d90b9d2d75", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00266.25", "name": "Aminotran_5", "length": 133, "aa_cov": 0.8410596026490066, "hmm_cov": 0.9924812030075187, "evalue": 3.242881851964724e-30, "score": 100.40299987792969, "start": 7, "stop": 133 } ], "seq_stats": { "molecular_weight": 17636.471600000004, "isoelectric_point": 9.49923915863037 }, "id": "KMADJIBOEG_774", "locus": "GCA000292445_00770" }, { "type": "cds", "sequence": "contig_1", "start": 422205, "stop": 422621, "strand": "+", "frame": 3, "gene": "iscU", "product": "putative protein MG337", "db_xrefs": [ "COG:COG0822", "COG:O", "GO:0005506", "GO:0016226", "GO:0051536", "RefSeq:WP_009885968.1", "SO:0001217", "UniParc:UPI0000139647", "UniRef:UniRef100_P47579", "UniRef:UniRef50_P47579", "UniRef:UniRef90_P47579" ], "nt": "ATGGATATTAGAGCAAGAACAAAAATTATTGATATTTATTCAAATTTAAAATACAAAAAACCTCTCAAAAGTTTTCAAAAAATTTTAACAACAAGTGATAGTGATAATTGTGAAGACTTTTTTAATATAGGTTTAAATATTGATAAAAATAAAATTACTGCAATTGGTTTTGATGGTGATGGTTGTATTATCTCTACAATTGCAACTGAATTAAGTATTAAAGCAATTGAAAATAAAACTATTAACCAAGCAAAAAAAATATTATCAGATTTAATTGCAACTTATAAAGATAAAAATTCAGCAAACCAAGTTGTTGAAGAATTAAAGCTTTTAATTGAAATGAATGTAACTGAAAAAAGGTTACAGTGTTTGCTTTTAACACCAAGCAACTTATTGCAATGGTTTAAAAATTTTTAG", "aa": "MDIRARTKIIDIYSNLKYKKPLKSFQKILTTSDSDNCEDFFNIGLNIDKNKITAIGFDGDGCIISTIATELSIKAIENKTINQAKKILSDLIATYKDKNSANQVVEELKLLIEMNVTEKRLQCLLLTPSNLLQWFKNF", "aa_hexdigest": "0dc246878c31421d62ae0f5649a611e9", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139647", "ncbi_nrp_id": "WP_009885968.1", "uniref100_id": "UniRef100_P47579", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139647", "RefSeq:WP_009885968.1", "UniRef:UniRef100_P47579" ] }, "ips": { "uniref100_id": "UniRef100_P47579", "uniref90_id": "UniRef90_P47579" }, "psc": { "uniref90_id": "UniRef90_P47579", "gene": "IscU", "product": "Uncharacterized protein MG337", "uniref50_id": "UniRef50_P47579", "cog_id": "COG0822", "cog_category": "O", "go_ids": [ "GO:0005506", "GO:0051536", "GO:0016226" ] }, "pscc": { "uniref50_id": "UniRef50_P47579", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47579" ], "product": "Uncharacterized protein MG337" }, "genes": [ "iscU" ], "id": "KMADJIBOEG_775", "locus": "GCA000292445_00771" }, { "type": "cds", "sequence": "contig_1", "start": 422608, "stop": 422784, "strand": "-", "frame": 1, "gene": null, "product": "DUF3713 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FDF155B", "UniRef:UniRef90_UPI002FDF155B" ], "nt": "ATGTCTTTACTTATTGAACAAGCTTTAGATGAAGGCACTCAAGAGTTGGCATTTTTAGATTTAATTCGTGAAAATCAAAAGCAACACCAAAGAGAACATCAACAGATTTTAGTTGGTGATAAACGCTTATATGATATCCTAGGACAAACGTTAGCTATTAATGCTAAAAATTTTTAA", "aa": "MSLLIEQALDEGTQELAFLDLIRENQKQHQREHQQILVGDKRLYDILGQTLAINAKNF", "aa_hexdigest": "94939ef73fd11265fe013b81829fdd69", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 422608, "stop": 423042, "observations": { "mutation": [ 422832 ], "stop": [ 422832 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FDF155B", "query_cov": 1.0, "subject_cov": 0.4027777777777778, "identity": 1.0, "score": 111.0, "evalue": 2.89e-30, "gene_start": 1, "gene_end": 58, "reference_start": 87, "reference_end": 144, "reference_sequence": "MLNVVDRLDDLYFHKFEGYVGLSKAANTDGSNTEGYKTVQTVPLTNDSSIQTLSYVIQITNDDVNKISSNWKNNKANSLGLKKEIFMSLLIEQALDEGTQELAFLDLIRENQKQHQREHQQILVGDKRLYDILGQTLAINAKNF" }, "paralog": false, "identity": 0.9861111111111112, "score": 279.0, "evalue": 5.83e-99, "description": "Internal stop codon at 422832. Nonsense mutation around 422832.", "psc": { "uniref90_id": "UniRef90_UPI002FDF155B", "product": "DUF3713 domain-containing protein", "uniref50_id": "UniRef50_UPI002FDF155B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FDF155B", "UniRef:UniRef50_UPI002FDF155B" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FDF155B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FDF155B" ], "product": "DUF3713 domain-containing protein" } }, "id": "KMADJIBOEG_776", "locus": "GCA000292445_00772" }, { "type": "cds", "sequence": "contig_1", "start": 423036, "stop": 423185, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAATAAAACCAGTTCTGATGCTGAACTTAATGCTTTTGGTGAGTTATTACATCGCTCAGTTAATGTTGATACAAATAATTTAAGTCGCTTTAACTCTAGAGGTGAACCTTTAATAAGTTTTGATAATAAAAAAAGTTTTTATTAA", "aa": "MINKTSSDAELNAFGELLHRSVNVDTNNLSRFNSRGEPLISFDNKKSFY", "aa_hexdigest": "226f0a1671754c802c5d5fa916fd6964", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5578.103099999999, "isoelectric_point": 6.531220817565917 }, "id": "KMADJIBOEG_777", "locus": "GCA000292445_00773" }, { "type": "cds", "sequence": "contig_1", "start": 423189, "stop": 423446, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGAAAGTGAAAACACTTATAGATTTACTGATGAACCGTTTAATAATTCTGTAGCTTTATCTAATAAATCACAAGGATCAAGTGATAAAAAATATTTTTATGGATTTAATGGTTTAACAATTAATTCCAATCAAAGTATTTCAACAGCTAGTGCTGGTTTAACACAACAGCTTTTTAATAATTACGGACAGTTAATTACAGCAACTGATAAAGCAGGCGCTTTAAGTCAATATAAGGATAAGTTTACCTTGTGA", "aa": "MLESENTYRFTDEPFNNSVALSNKSQGSSDKKYFYGFNGLTINSNQSISTASAGLTQQLFNNYGQLITATDKAGALSQYKDKFTL", "aa_hexdigest": "ac5100230865ba6ce0437b357380d75e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9393.166299999992, "isoelectric_point": 6.079976081848144 }, "id": "KMADJIBOEG_778", "locus": "GCA000292445_00774" }, { "type": "cds", "sequence": "contig_1", "start": 423699, "stop": 423857, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCAAATGCAAAATAAAGCTGCCCAATCTCATACTTCTAGTAGTGTTAATCAAACCCTATTAGATTTAGCAAAGAAAGCATTTGAAAAGGAATTGGAAGATCCTAATCAGGATGCTGAGTACAAATACATGCGCTTTTTGCAAGCGTTAATGTGA", "aa": "MLQMQNKAAQSHTSSSVNQTLLDLAKKAFEKELEDPNQDAEYKYMRFLQALM", "aa_hexdigest": "76498baee34a784911fae9d47657d9b2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6035.8148, "isoelectric_point": 5.6171361923217775 }, "id": "KMADJIBOEG_779", "locus": "GCA000292445_00775" }, { "type": "cds", "sequence": "contig_1", "start": 423927, "stop": 424124, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATCTTGATCTTATATTATCACTTTTAACTGATTCAGGAATTAGAACAGGTACTGTTGCAAACACCTTTAAAAATTGGTACTTTAAAAACACTAATTCATTCACTAATAATTTTGATACTTCAAATAAAGAATTAAAAGGTGAATTTTCTGATTTTAATGATCTTGTTAAACAGGCACTTTATATCCGTTCTTGA", "aa": "MDLDLILSLLTDSGIRTGTVANTFKNWYFKNTNSFTNNFDTSNKELKGEFSDFNDLVKQALYIRS", "aa_hexdigest": "d1a61a0853a8d07dc47d810fe88755a3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7474.2420999999995, "isoelectric_point": 5.03322925567627 }, "id": "KMADJIBOEG_780", "locus": "GCA000292445_00776" }, { "type": "cds", "sequence": "contig_1", "start": 424194, "stop": 424712, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGAAAATGGAACTTCTAATAATAACGGTCAAAAAGATGTATGTGATTTAGCTAAAAAACTTTTAAAAAACAATACCAATTTATCTGAAACTATTAAAAAACAACAAGATTTTAATAATTCACTTTCACAAATAAAATCAAGTTATGAGTCTTATATTAAAGCTGCTAATAAACTTAATAACTTTAGAACAGATTTAGCTAATATAGAAAAACTAGAACAAGCAATTGTTGATCGTGCTAACAACTACATTAAACTTCAAAAGGAAGCTAAAGAAAGTTCAATTGGTTGGGGTCAACCTCTTCCATATAAAAGAGCAAATGATGGTAGTTATCCTTCATTAGCAAAATTTTTTAATAACAACTCTGATCAAAGTAGTGCTCAAACTTTAACTTTGAAAACCACTGCTGCAGCTATTACTTCTGATAATGAACCAACTCGTGAAAAAAATAATCAAACTTTAAAGACTTTAACAACTGAAGTAGAAAATAAAGCCAAAGAATTAGTTGAAAAATGA", "aa": "MLENGTSNNNGQKDVCDLAKKLLKNNTNLSETIKKQQDFNNSLSQIKSSYESYIKAANKLNNFRTDLANIEKLEQAIVDRANNYIKLQKEAKESSIGWGQPLPYKRANDGSYPSLAKFFNNNSDQSSAQTLTLKTTAAAITSDNEPTREKNNQTLKTLTTEVENKAKELVEK", "aa_hexdigest": "8aeecd8dd1ab99f285420dd9af9bdee1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19280.205400000006, "isoelectric_point": 9.025138664245606 }, "id": "KMADJIBOEG_781", "locus": "GCA000292445_00777" }, { "type": "cds", "sequence": "contig_1", "start": 424758, "stop": 425063, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATCAAATTCATCTCAAGCACAAGTAGTAACAAGTAATTTATTAGTTAGTCTAAGTTCAAAGACAACTCAAAAACAGCAACAGAAACCTGTTTATGTTAGAGGTGATGATGCCATATATGCATTTCATATTGATGGAGGTAATTATTTTTTAGAGAATTCATCACCTAACAAACGTAATTTTGAAAAACAAGCAGAAGTTTTATTAATGCGATTTTTACAAGGACAAACTAATAATTTCTCTAAAGATAATGTCAGTTTTTCAGTTGATTTATTTGGTTCTAACAGCGAATTTAGAAGTTGA", "aa": "MESNSSQAQVVTSNLLVSLSSKTTQKQQQKPVYVRGDDAIYAFHIDGGNYFLENSSPNKRNFEKQAEVLLMRFLQGQTNNFSKDNVSFSVDLFGSNSEFRS", "aa_hexdigest": "7dbc9403cfa7e941b6890b1c8db80b6b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11392.426599999992, "isoelectric_point": 6.553274345397949 }, "id": "KMADJIBOEG_782", "locus": "GCA000292445_00778" }, { "type": "cds", "sequence": "contig_1", "start": 425124, "stop": 425264, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGAAGAGTGCTATGACAAAAAGTCAAGAAGTTACTTCAGACAATAATGGTTTTAATGTTAAAAGTGAATTTTTAAAAATTAATCCCTCGCTTTCTTCTTCTGGTAGTGATAATAGTAGTAACACGCAGTCTTTTTGA", "aa": "MMKSAMTKSQEVTSDNNGFNVKSEFLKINPSLSSSGSDNSSNTQSF", "aa_hexdigest": "6956f923ce8b266dcbd79b6fcabb3f5e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4963.360500000002, "isoelectric_point": 5.971186256408691 }, "id": "KMADJIBOEG_783", "locus": "GCA000292445_00779" }, { "type": "cds", "sequence": "contig_1", "start": 425443, "stop": 425607, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTATTTTTACTAATTAAAACTTTGCTTGCACTATACTTGTTTTCAAAGTCAAGTAAATCATTTCTGGTATTAGTAGCAGCTGAACTTTGTGATGAACCACTAGAATTAGCAGTTTGTAATTGTTTATCAAACTCTTCAAATAATTTTGCAGAGTTTGTTTGA", "aa": "MLFLLIKTLLALYLFSKSSKSFLVLVAAELCDEPLELAVCNCLSNSSNNFAEFV", "aa_hexdigest": "29d48c29d2fb0bf6049ef0aff8176cfc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5974.032999999998, "isoelectric_point": 4.584314918518066 }, "id": "KMADJIBOEG_784", "locus": "GCA000292445_00780" }, { "type": "cds", "sequence": "contig_1", "start": 425769, "stop": 425912, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATTCAAGTGATACAAGCGTTGCAACAACTAAAGAAAACATTGAAAAAACAGAAAATAAACTTAAGTTTATTAACAGCAATTTAAATGATGTAAATGGTGATTTTTTTACCAATCTTCAAGCTTATTTATTTTCACAGTGA", "aa": "MNSSDTSVATTKENIEKTENKLKFINSNLNDVNGDFFTNLQAYLFSQ", "aa_hexdigest": "19d2d05f462909308cc65baeeb6a2ea3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5332.773000000001, "isoelectric_point": 4.593750190734863 }, "id": "KMADJIBOEG_785", "locus": "GCA000292445_00781" }, { "type": "cds", "sequence": "contig_1", "start": 426165, "stop": 426368, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGTGCAACAGCAATAATTACAGCCTGTTCCACAATAAACACTGCTAATCTTTTTCCTACTCTCAATCGATCTCAAAAACTAATTGGTTTTAGTGATCAAAATATCATAAACCCAGATGTAGTTTTAAAGTCTGCTTTAAATGATGGTAGTGGTGTTGATTCTATCTTACGTGTTAGTTTTGGATCAGCATTACAAAAATGA", "aa": "MGATAIITACSTINTANLFPTLNRSQKLIGFSDQNIINPDVVLKSALNDGSGVDSILRVSFGSALQK", "aa_hexdigest": "8d840155f7d4cb0b7eb1aebb2784c033", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7008.939300000002, "isoelectric_point": 7.934011268615723 }, "id": "KMADJIBOEG_786", "locus": "GCA000292445_00782" }, { "type": "cds", "sequence": "contig_1", "start": 426597, "stop": 427619, "strand": "-", "frame": 2, "gene": "recA", "product": "recombinase RecA", "db_xrefs": [ "COG:COG0468", "COG:L", "GO:0003677", "GO:0003684", "GO:0003697", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0008094", "GO:0009432", "GO:0016887", "GO:0140664", "KEGG:K03553", "RefSeq:WP_009885970.1", "SO:0001217", "UniParc:UPI00001334FA", "UniRef:UniRef100_P47581", "UniRef:UniRef50_P78014", "UniRef:UniRef90_P47581" ], "nt": "ATGGCTCAAAAAGAAATAATTAATAAGAAAAATACTCAAAAAAATAGTAGTTTTATTGAAAGTAATAATTTGACAAGTTTTGATTTTTTTGATGCAAAGAAAAACAGTGAAATTGAAACAATTTCAACTGGAAGTTTAAATTTAGATGAAGCATTAGGGTCTGGTGGTCTGCCTTTAGGTAGGATAGTAGAACTATATGGAAATGAATCATCTGGAAAAACAACTATTGCACTAAATGCAGTCGCTAGTTTTCAGAAAGCAGGTAAAACAGCCTGTTATATTGATGCTGAAGGTGCACTTGATTTAGCATATGCTAAATCAATTGGTATTGATCTAAATAAACTTTTGATTGCTCATCCTAGGCATGGTGAAAACGCTTTTGCTCTTATCGAATCATTAATTAAAACAAACAAGATATCTTTAATTGTTATTGACTCTGTAGCAGCGTTAATTCCTAAACAAGAGCTAGAAGGCACCATTGAAGAACAAACTATTGGCTTGCATGCAAGAATGATGTCAAAAGGTTTGCGAAGAATACAATCGATATTACCAGATTCTAAAACTTGTGTTTTATTCATTAATCAGTTACGCGAAAAACCAGGAGTGATGTTTGGAAATAACGAAGTTACAACAGGAGGAAAAGCTCTAAGATTTTATAGTTCATTAAGAATGGAAGCTAAGCGTGTTGAATTACTTAAGGATAAATTCAACAATTATGTTGGCATAAAAACAAAAGTAATGGTATCTAAAAATAAGATTGCTAAACCTTTTGGTGTTGCTATATTAGAAATCATGTTTAACCGTGGTTTTGTACATGAACATGAAGTTATTGACTTAGCACTTAAATTTAATGTTGTTGTAAGAGCTGGAAATTCTTATTCTTTTAACAATGAAAGCATTGCTGTTGGTAAAGAAAAATTATTAAATGTTTTATCAGAAAAGCCAGCATTATTTGAACAAATAAAAGAACTAACTGTTCAACAATTGGCTAATAAAAATTCATTTCAACAAACAGCTAGCTAA", "aa": "MAQKEIINKKNTQKNSSFIESNNLTSFDFFDAKKNSEIETISTGSLNLDEALGSGGLPLGRIVELYGNESSGKTTIALNAVASFQKAGKTACYIDAEGALDLAYAKSIGIDLNKLLIAHPRHGENAFALIESLIKTNKISLIVIDSVAALIPKQELEGTIEEQTIGLHARMMSKGLRRIQSILPDSKTCVLFINQLREKPGVMFGNNEVTTGGKALRFYSSLRMEAKRVELLKDKFNNYVGIKTKVMVSKNKIAKPFGVAILEIMFNRGFVHEHEVIDLALKFNVVVRAGNSYSFNNESIAVGKEKLLNVLSEKPALFEQIKELTVQQLANKNSFQQTAS", "aa_hexdigest": "de552d34d44dec5d67f423ae90a06d72", "start_type": "ATG", "rbs_motif": "TTATA", "ups": { "uniparc_id": "UPI00001334FA", "ncbi_nrp_id": "WP_009885970.1", "uniref100_id": "UniRef100_P47581", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001334FA", "RefSeq:WP_009885970.1", "UniRef:UniRef100_P47581" ] }, "ips": { "uniref100_id": "UniRef100_P47581", "uniref90_id": "UniRef90_P47581" }, "psc": { "uniref90_id": "UniRef90_P47581", "gene": "recA", "product": "recombinase RecA", "uniref50_id": "UniRef50_P78014", "cog_id": "COG0468", "cog_category": "L", "kegg_orthology_id": "K03553", "go_ids": [ "GO:0003677", "GO:0003684", "GO:0003697", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0008094", "GO:0009432", "GO:0016887", "GO:0140664" ] }, "pscc": { "uniref50_id": "UniRef50_P78014", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78014" ], "product": "Protein RecA" }, "genes": [ "recA" ], "id": "KMADJIBOEG_787", "locus": "GCA000292445_00783" }, { "type": "cds", "sequence": "contig_1", "start": 427955, "stop": 428131, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13", "UniRef:UniRef90_UPI002FCE54A6" ], "nt": "GTGGTGAAAAAGTATCAGTTAGATGTGCCAAGTGTTACCAATGAGATGAACCAAGTGTTGCAAGAATTGATCCTAGAACAACCTTTGACTAAATATACCTTAAACAGTAGTTTGGCCAAGCAAAAGGGTAAGACACAAGCAGTGGTGCATCTTGGTTCAAATATGACAAATAATTGA", "aa": "MVKKYQLDVPSVTNEMNQVLQELILEQPLTKYTLNSSLAKQKGKTQAVVHLGSNMTNN", "aa_hexdigest": "e347e189eaca3a1798c824f4e35df535", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 427814, "stop": 428196, "observations": { "deletion": [ 428153 ], "mutation": [ 427949, 428129 ], "stop": [ 427949, 428129 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FCE54A6", "query_cov": 0.896551724137931, "subject_cov": 0.41935483870967744, "identity": 0.885, "score": 92.8, "evalue": 4.59e-23, "gene_start": 1, "gene_end": 52, "reference_start": 47, "reference_end": 98, "reference_sequence": "MLFKSTQVKLTYSSSGSNNISFENNANKPSYVVEFTNSTTVGIKWSVVKKYQLDVPNVTNEMNQVLQELILEQPLTKYTLNGSLAKQKGKSQVAVHLGGQASQWHSTRHSQSKQQSQPQGFNRI" }, "paralog": false, "identity": 0.7322834645669292, "score": 159.0, "evalue": 4.51e-52, "description": "Internal stop codon at 427949, 428129. Frameshift due to deletion around 428153. Nonsense mutation around 427949, 428129.", "psc": { "uniref90_id": "UniRef90_UPI002FCE54A6", "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FCE54A6", "UniRef:UniRef50_UPI00027B3D13" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6517.464900000003, "isoelectric_point": 9.158652687072756 }, "id": "KMADJIBOEG_788", "locus": "GCA000292445_00784" }, { "type": "cds", "sequence": "contig_1", "start": 428259, "stop": 428864, "strand": "+", "frame": 3, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030D3153C", "UniRef:UniRef90_UPI0030D3153C" ], "nt": "ATGGGACCAAGCACGGCAAAGGGAAGGATAGTAATGGGTGGAGTTCAACAGAAGAAAACAATGGCAGCAGGGGATGCGCCCAGTGTTACAGGAGGGGGAACATCAGACAATTCTTCAAAATTCCAAAGTTACCTCAACACCAAACAAGCGTTAGAAAGCATCGGTATCTTGTTTGATGGGGATGGAATGAGGAATGTTATCACCCAGCTCTATTATGCTTCAACTAGTAAGCTAGCAGTCACTAACAACCACATTGTCGTGATGGGTAACAGCTTTCTACCCAGCATGTGGTACTGGGTGGTGGATAGGAGTGCAACAACTGATTCATCATCAAAACCCACCTGGTTTGCTAACACCAATTTAGATTGGGGAGAAGACAAACAAAAACAATTTGTAGAGAATCAGTTGGGGTATAAGGAAACTACCAGTACCAATTCCCACAACTTCCATTCCAAATCTTTCACCCAACCTGCATATCTGATCAGTGGCATTGACAGTGTCAATGATCAAATCATCTTCAGTGGTTTTAAAGCGGGCAGTGTGGGGTATGATAGTAGTAGTAGTAGTAGTAGTAGTACTCAAACCAAAGACCAAGCACTGGCTTGA", "aa": "MGPSTAKGRIVMGGVQQKKTMAAGDAPSVTGGGTSDNSSKFQSYLNTKQALESIGILFDGDGMRNVITQLYYASTSKLAVTNNHIVVMGNSFLPSMWYWVVDRSATTDSSSKPTWFANTNLDWGEDKQKQFVENQLGYKETTSTNSHNFHSKSFTQPAYLISGIDSVNDQIIFSGFKAGSVGYDSSSSSSSSTQTKDQALA", "aa_hexdigest": "ceb1287290b0bcfd8aa83ffa8d8bfeb1", "start_type": "ATG", "rbs_motif": null, "genes": [ "mgpC" ], "truncated": "3-prime", "pseudogene": { "start": 428349, "stop": 429164, "observations": { "mutation": [ 428862 ], "stop": [ 428862 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0030D3153C", "query_cov": 0.8507462686567164, "subject_cov": 0.5625, "identity": 0.895, "score": 297.0, "evalue": 8.6e-99, "gene_start": 31, "gene_end": 201, "reference_start": 1, "reference_end": 171, "reference_sequence": "GGGGSDTASKFTKYLNTKQALERIGILFDGEKARNVITQLYYASTSKLAVTNNHIVVMGNSFLPSLWYWVVDRGATTDSSSKPTWFANTNLDWGEDKQKQFVENQLGYKETTSTNSHNFHSKSFTQPAYFISGIDSVNDQLIFSGFKAGSVGYDSSSSSSSSSSTKDQALAWSTTTSLDSKTGYTDLVTNDTGLNGPINGSFSIQDTFSFVVPYSGNHSNQTSSGPIKTAYPVKKSEASSEAINSLINATPLNSYGYRYIFLLWSHPKVEKRESSEGFKQRQWETQFNKVIHEWGYIHSQSPQG" }, "paralog": false, "identity": 0.8597785977859779, "score": 455.0, "evalue": 1.56e-163, "description": "Internal stop codon at 428862. Nonsense mutation around 428862.", "psc": { "uniref90_id": "UniRef90_UPI0030D3153C", "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI0030D3153C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0030D3153C", "UniRef:UniRef50_UPI0030D3153C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0030D3153C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030D3153C" ], "product": "MgpC family cytadherence protein" } }, "id": "KMADJIBOEG_789", "locus": "GCA000292445_00785" }, { "type": "cds", "sequence": "contig_1", "start": 429063, "stop": 429311, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGCAACGGCTTTTTGGCAAAGTCGTTTTTTTTTCCACCTCTATCTTAAGGGCCTTGGCTCGAGTGGATGTAGCCCCACTACTCATCGATGAAGTTGCTTCACTGAGTTTCTCCCCTGTTTTACTTGAATCGCTTTCTGGACTCCCACTTTGCAACCTTTGGGTGGGGTTGAAGGTGGAAGTTGCTTCCACTTCCATAACTATTCAACGGCGTAGCGTTGATCAAGGAATTGATCTTGACAGTTGA", "aa": "MLQRLFGKVVFFSTSILRALARVDVAPLLIDEVASLSFSPVLLESLSGLPLCNLWVGLKVEVASTSITIQRRSVDQGIDLDS", "aa_hexdigest": "78a1627668665212f950c2677a0e7afb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8916.365899999993, "isoelectric_point": 5.086430549621581 }, "id": "KMADJIBOEG_790", "locus": "GCA000292445_00786" }, { "type": "cds", "sequence": "contig_1", "start": 429391, "stop": 429561, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCATTGATTTAACCCCAACATTGATGTTAGAAAAAGTGGGGAGTGGGGTTGAGGAAGAAGAAGAAGTAGAAGAAGAAGAAGAAGAAGAAGAAGAAGCAGAAGTTGAAGGGGAGGGGGAGTTGGAGAAGAAGCCCCCATCCCCTTCTCTCTTGCTATTTGCTCGGTAG", "aa": "MIIDLTPTLMLEKVGSGVEEEEEVEEEEEEEEEAEVEGEGELEKKPPSPSLLLFAR", "aa_hexdigest": "8415934f8a1e7ab361f12ea40b0be13c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6302.854999999998, "isoelectric_point": 4.0500284194946286 }, "id": "KMADJIBOEG_791", "locus": "GCA000292445_00787" }, { "type": "cds", "sequence": "contig_1", "start": 429630, "stop": 430013, "strand": "+", "frame": 3, "gene": null, "product": "Random genomic clone esh10, partial cds", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49206" ], "nt": "GTGCAGGTTATAGAAACAGCTAATGACAACAATAACGGTATCTCCTTTGAAATGGTGAAGCCTAGCAATAGTAGTACCCCCTTTAATCCCAATTCTTCTGAAAACCAAGTCACACCATCAGGTAGCTCCCCCAAAAAAACTACATATGATTTTTTACCCAACAGTATCAGTCCCACCAGTGACTGGATCAATGCGTTGACTTTCACTAATAAGAATAACCCGCAACGAAATCAGTTGTTGCTCAGAAGCTTACTAGGAACGATCCCGGTATTGATCAATAAGAGTGGAACGGGAGATGAGTTTAACCATACGAGTGACCAGAAGTGGGATAAAACTGAGACAAAAGATGGGAATTTCCCTGGGTTTGGGGAGGTGAATGGGTGA", "aa": "MQVIETANDNNNGISFEMVKPSNSSTPFNPNSSENQVTPSGSSPKKTTYDFLPNSISPTSDWINALTFTNKNNPQRNQLLLRSLLGTIPVLINKSGTGDEFNHTSDQKWDKTETKDGNFPGFGEVNG", "aa_hexdigest": "3de4bd32f97620d7eb3fed0a0b91c666", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0029624762", "query_cov": 0.8661417322834646, "subject_cov": 0.9016393442622951, "identity": 0.836, "score": 174.0, "evalue": 2.73e-54, "valid": false, "product": "adhesin", "uniref50_id": "UniRef50_Q49206" }, "pscc": { "uniref50_id": "UniRef50_Q49206", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49206" ], "product": "Random genomic clone esh10, partial cds" }, "genes": [], "id": "KMADJIBOEG_792", "locus": "GCA000292445_00788" }, { "type": "cds", "sequence": "contig_1", "start": 430037, "stop": 431560, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05000.22", "PFAM:PF04998.22" ], "nt": "GTGAAAGAAAAAGTATCTGATGAGATCCAAAGCTTAATTAAACAAGAACAGTACCGTGATAATTCCATTGTGGTAATGGCTGATTCAGGCGCTAGAGGTAACATTTCTAACTTTACCCAGTTATTTGGGATGCGCGGCTTAATGTCTAAAAGCTTTAACTATGAAAGAAATAACCAATCTAAGATCATTAAAGATACGATAGAAGTTCCTATTAAACACTCCTTTTTTGAAGGTTTGACCATTAATGAATACTTCAACTCTTCTTATGGAGCGAGAAAAGGGATGACAGATACTGCAATGAAAACAGCAAAGTCTGGTTATATGACAAGAAAGCTAGTAGATGCTACTCATGAATTAATTATTAACCATGATGATTGTGGAACAAGAAAAGGAATTGTTGTTGAAGCAATTGTTGAAACCAAAACCAAATCCTTGATTGAATCATTATTTGACAGGATTGTTAATCGCTACTCAATTACTCCTATAGTTGATCCTGAAACACAAAAAACTATTGTAGAAACTAACAGTCTTATTACAACGCAATTAGCTAAACAGATTTGTGCAACATCTATTAAGGAAGTTTTAGTTAGATCTGTTATCTATTGTGAAAAGGAAAATGGTATCTGTCAATACTGCTTTGGCATTGACTTGTCAACTGGTAAGTTGGTGGAATTGGGAACTGCTGTTGGGGTGATTGCTGCTCAATCAATTGGTGAACCTGGGACCCAATTGACAATGCGTACTTTCCATACTGGGGGAGTTTCAACTGAAAACAACTTAGCACAAGGCTTTGAACGTTTAAAACAGATCTTTGAAGTAGTTACTCCTAAAGATTTTGAAAAAGCAGTTATCTCTGAAGTGAAAGGAACAGTTAAATCAATTACTACTGTTCAAAACGCTCAGGAAGTAGTGATTAAATCAAACGTTGATGAGAGGATTTATACTATCCCTTTCAGTGCTCAAATACGTGTTCATGTTGGTGATCAAGTTTCACCAGGTTCTAAGATTACAGAAGGTTCTGTTGATATTAAACAACTTTTGCGAATTGCAGGTATCCAAAGGGTAAGACAATACATGATTGTAGAGATCCAAAAAGTGTATAGGATTCAAGGGATAGATATTGCTGATAAGTATGTAGAAATTATTATCAGACAACTAACTAATTTGTTGCAAGTAACAGATGCAGGTAACAGCAATTTATTTGTTGGTCAGTTAGTGCATAGCCATTATCTCAATGAACTAAATAAGAGCTTACTTTTAGCTGGAAAGATGCCTGTTATTGCAATTAATCAGGTGTTTGGAATTGATGAAGCGGCAAGTAAATCTAACTCCTTTTTAAGTGCTGCATCCTTCCAAGATACCAAAAAAATCCTAACTGATGCTGCTGTTAAGAACCAAGTAGACTATCTTTTAGGTTTAAAAGAGAATGTTATTATTGGTGGAAAAATTCCAGCAGGAACAGGATTTTTAACTGATGAAGAGTTAACTTTCTTAGGTAGCAAAACAGTTGCTGAAGAGTATTAA", "aa": "MKEKVSDEIQSLIKQEQYRDNSIVVMADSGARGNISNFTQLFGMRGLMSKSFNYERNNQSKIIKDTIEVPIKHSFFEGLTINEYFNSSYGARKGMTDTAMKTAKSGYMTRKLVDATHELIINHDDCGTRKGIVVEAIVETKTKSLIESLFDRIVNRYSITPIVDPETQKTIVETNSLITTQLAKQICATSIKEVLVRSVIYCEKENGICQYCFGIDLSTGKLVELGTAVGVIAAQSIGEPGTQLTMRTFHTGGVSTENNLAQGFERLKQIFEVVTPKDFEKAVISEVKGTVKSITTVQNAQEVVIKSNVDERIYTIPFSAQIRVHVGDQVSPGSKITEGSVDIKQLLRIAGIQRVRQYMIVEIQKVYRIQGIDIADKYVEIIIRQLTNLLQVTDAGNSNLFVGQLVHSHYLNELNKSLLLAGKMPVIAINQVFGIDEAASKSNSFLSAASFQDTKKILTDAAVKNQVDYLLGLKENVIIGGKIPAGTGFLTDEELTFLGSKTVAEEY", "aa_hexdigest": "3270ea72dcac551753f74ce3e810be3b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05000.22", "name": "RNA_pol_Rpb1_4", "length": 78, "aa_cov": 0.14595660749506903, "hmm_cov": 0.9871794871794872, "evalue": 1.0260302667661027e-17, "score": 59.17518997192383, "start": 2, "stop": 75 }, { "id": "PF04998.22", "name": "RNA_pol_Rpb1_5", "length": 339, "aa_cov": 0.6331360946745562, "hmm_cov": 0.7581120943952803, "evalue": 2.4630421062938776e-58, "score": 192.76177978515625, "start": 78, "stop": 398 } ], "seq_stats": { "molecular_weight": 56080.66890000005, "isoelectric_point": 6.69486026763916 }, "id": "KMADJIBOEG_793", "locus": "GCA000292445_00789" }, { "type": "cds", "sequence": "contig_1", "start": 431567, "stop": 433612, "strand": "-", "frame": 3, "gene": "rpoC", "product": "DNA-directed RNA polymerase subunit beta'", "db_xrefs": [ "COG:COG0086", "COG:K", "EC:2.7.7.6", "GO:0000287", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0008270", "KEGG:K03046", "SO:0001217", "UniRef:UniRef50_P47582", "UniRef:UniRef90_P47582" ], "nt": "ATGGGACATATTGCACTTGTGAGTCCTGTAGCTCACATTTGGATGTCAAAAGAATTACCATCTCCTTCCAAAATATCATTAGTTTTAAACATCTCTTACAAAGAGGTTGAACAGGTTTTGTACTTTGTTAACTACATAGTACTTGATACAGGTAAGATCAAAGATGATAAAATCATGCCTTTTAAGTTCAAAGAAGTTTTGGACTTAACTGGTAAGGGTTCACTTTCAACACGACAAAAAATGCGTCGTGTGATAGGTTATATCTTCAGAAATCTCATTAAAAGTAAGAGTAGTGAAGATTACCGTAAGGGAAAAATCTTTTATGAAAGTTTGAAAAACAGCTCTCTCCCCTTTTCTTTAAATGATGCTTTTAATTACATTAAGAAGTACACTGGTTTTAGGGTTGGAATAGGGGCTGAAGCAATTTTGGAATTGCTTAATAAAATCGATCTTAACTTGGAATTTAGCAGGTTAAATGATGCTTTAAGAAAAGCCAAGAAAGATAGTGTTGAAGATGCTAAAGTTAAGAAGATCTTAAGACAACTGGAAACTATTAGTTGGTTTAGAAATTCTAAGCTTCATCCTAAAAACATGATCTTACATACTGTTCCAGTTATCCCCCCTGATATCAGACCTATTATCCAACTTGATGGTGCTAAGTTTACCACCAGTGACATCAACAATTTTTACCGCAGGGTAATCATTAGAAATGACCGATTAAGAAGGATTTTGGAAGATGGTACTGTACCTTCTATTGTTGTTAACAATGAAAAAAGACTTTTACAAGAGTCTGTTGATGCTTTATTTGATAACTCTTCACGTCATAAACCATCACTTTCCAAAGACAAACGGTCATTGAAATCTTTAACAGATCGTTTAAAAGGAAAACAAGGTTTATTTAGACACAACTTACTTGGTAAAAGAGTTGATTATTCAGGTAGAAGTGTAATTGTGGTTGGCCCTGAATTGAAGATGTATGAAGTTGGGATCCCAGCATTAATGATCTTAAAGCTGTTTAAACCCTTTATTATCCATGGATTGATCAATAAGTTTGATGAAAATGGTAATGAGATTAGACCAATTGCCGCTTCCATCAGACAAGCTGAAGATATGATTAAAAACCAGGATGATCTTATCTGGGGAATAGTTTATGATGTTATCAAAGATCGTCCTGTTTTACTAAATCGTGCTCCAACCCTACATAGATTAGGGATCCAAGCATTTGAACCAAGAATTGTTGATGGTAAAGCAATTAGATTACACCCATTAGTAACTACTGCATTTAATGCTGATTTTGATGGTGATCAGATGGCAGTTCATGTTCCTTTAAGTGATAATGCAGTTAATGAAGCAAGAGCTGTTCTGCTTGCATCAAAACATATCTTAGGTTTGAAAGATGGAAGACCCATTGTAACTCCTACTCAGGACATGGTTTTAGGTAACTATTATCTAACAACAGAGAGAAAAGGACAGTTGGGAGAGGGGATTATCTTCAGCACAGTTTATGAAGCACGTGCTGCTTATGAAAGTCAAAAGGTTCATTTACATGCTATTGTAGGGATAAGTACTAAAGCATTTCCCAACAAGAAGTTTGCATGCCAAGGAACTTTAATAACAACAGTTGGTAAGATTATCTTTAATGATGTTTTAGGCAATAATGTTCCTTATATTAATGACGGGGAATTTGATGAAAATGCATGTCCTGAAAAGTTCATTGTGAAACAGGGAGAAGATGTAAGACAAGCAATTTTAAAGCATCAAATTATCCCTGCATTTTCCAAAAAGGTTATTTCCAAGTTAATTGATCTACTCTATCTTTTATTGGAATTTAAAGATCTTCCTAAAACACTTGATAATATCAAAGCACTTGGCTTTAAGTACTCTACTTTTTCTTCAACTACTGTTTCAGTATTTGATATCCCTAAGTACACTAATAAACAAAATTACTTTGATAGTGCTGATCAACAGGTGCTGAAATACAAACAGTTTTATAACAAGGGTTTGTTAACCGATGATGAACGTTATAAACGCGTTGTGAAGTTATGA", "aa": "MGHIALVSPVAHIWMSKELPSPSKISLVLNISYKEVEQVLYFVNYIVLDTGKIKDDKIMPFKFKEVLDLTGKGSLSTRQKMRRVIGYIFRNLIKSKSSEDYRKGKIFYESLKNSSLPFSLNDAFNYIKKYTGFRVGIGAEAILELLNKIDLNLEFSRLNDALRKAKKDSVEDAKVKKILRQLETISWFRNSKLHPKNMILHTVPVIPPDIRPIIQLDGAKFTTSDINNFYRRVIIRNDRLRRILEDGTVPSIVVNNEKRLLQESVDALFDNSSRHKPSLSKDKRSLKSLTDRLKGKQGLFRHNLLGKRVDYSGRSVIVVGPELKMYEVGIPALMILKLFKPFIIHGLINKFDENGNEIRPIAASIRQAEDMIKNQDDLIWGIVYDVIKDRPVLLNRAPTLHRLGIQAFEPRIVDGKAIRLHPLVTTAFNADFDGDQMAVHVPLSDNAVNEARAVLLASKHILGLKDGRPIVTPTQDMVLGNYYLTTERKGQLGEGIIFSTVYEARAAYESQKVHLHAIVGISTKAFPNKKFACQGTLITTVGKIIFNDVLGNNVPYINDGEFDENACPEKFIVKQGEDVRQAILKHQIIPAFSKKVISKLIDLLYLLLEFKDLPKTLDNIKALGFKYSTFSSTTVSVFDIPKYTNKQNYFDSADQQVLKYKQFYNKGLLTDDERYKRVVKL", "aa_hexdigest": "dfe0c78f105cd32f59bff776f57285f2", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpoC" ], "truncated": "both", "pseudogene": { "start": 431264, "stop": 433912, "observations": { "mutation": [ 431569, 433645, 433819 ], "stop": [ 431569, 433645, 433819 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47582", "query_cov": 1.0, "subject_cov": 0.5270897832817337, "identity": 0.997, "score": 1322.0, "evalue": 0.0, "gene_start": 1, "gene_end": 681, "reference_start": 102, "reference_end": 782, "reference_sequence": "MTTTRRNKRNNKLYKNIKAIKLSIASNDTILNWSEGEVTKAETINYKSLKPEPGGLFDEAIFGPVKDYECACGKFKKIKYRGVRCDRCGVWVTESIVRRERMGHIALVSPVAHIWMSKELPSPSKISLVLNISYKEVEQVLYFVNYIVLDTGKIKDDKIMPFKFKEVLDLTGKGSLSTRQKMRRVIGYIFRNLIKSKSSEDYRKGKIFYESLKNSSLPFSLNDAFNYIKKYTGFRVGIGAEAILELLNKIDLNLEFSRLNDALRKAKKDSVEDAKVKKILRQLETISWFRNSKLHPKNMILHTVPVIPPDIRPIIQLDGAKFTTSDINNFYRRVIIRNDRLRRILEDGTVPSIVVNNEKRLLQESVDALFDNSSRHKPSLSKDKRSLKSLTDRLKGKQGLFRHNLLGKRVDYSGRSVIVVGPELKMYEVGIPALMILKLFKPFIIHGLINKFDENGNEIRPIAASIRQAEDMIKNQDDLIWGIVYDVIKDRPVLLNRAPTLHRLGIQAFEPRIVDGKAIRLHPLVTTAFNADFDGDQMAVHVPLSENAVNEARAVLLASKHILGLKDGRPIVTPTQDMVLGNYYLTTERKGQLGEGIIFSTVYEARAAYESQKVHLHAIVGISTKAFPNKKFACQGTLITTVGKIIFNDVLGNNVPYINDGEFDENACPEKFIVKQGEDVRQSILKHQIIPAFSKKVISKLIDLLYLLLEFKDLPKTLDNIKALGFKYSTFSSTTVSVFDIPKYTNKQNYFDSADQQVLKYKQFYNKGLLTDDERYKRVVKLWNNVKEKVSDEIQNLIKQEQYRDNSIVVMADSGARCNISNFTQLFGMRGLMSKSFNYERNNQSKIIKDTIEVPIKHSFFEGLTINEYFNSSYGARKGMTDTAMKTAKSGYMTRKLVDATHELIINHDDCGTRKGIVVEAIVETKTKSLIESLFDRIVNRYSITPIVDPETQKTIVEANSLITTQLAKQICATSIKEVLVRSVIYCERENGICQYCFGIDLSTGKLVELGTAVGVIAAQSIGEPGTQLTMRTFHTGGVSTENNLAQGFERLKQIFEVVTPKDFEKAVISEVKGTVKSITTVQNAQEVVIKSNVDERIYTIPFSAQIRVHVGDQVSPGSKITEGSVDIKQLLRIAGIQRVRQYMIVEIQKVYRIQGIDIADKYVEIIIRQLTNLLQVTDAGNSNLFVGQLVHSHYLNELNKSLLLAGKMPVIAINQVFGIDEAASKSNSFLSAASFQDTKKILTDAAVKNQVDYLLGLKENVIIGGKIPAGTGFLTDEELTFLGSKTVAEEY" }, "paralog": false, "identity": 0.9920634920634921, "score": 1722.0, "evalue": 0.0, "description": "Internal stop codon at 431569, 433645, 433819. Nonsense mutation around 431569, 433645, 433819.", "psc": { "uniref90_id": "UniRef90_P47582", "gene": "rpoC", "product": "DNA-directed RNA polymerase subunit beta'", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P47582", "cog_id": "COG0086", "cog_category": "K", "kegg_orthology_id": "K03046", "go_ids": [ "GO:0000287", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0008270" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47582", "EC:2.7.7.6", "UniRef:UniRef50_P47582", "COG:COG0086", "COG:K", "KEGG:K03046", "GO:0000287", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0008270" ] }, "pscc": { "uniref50_id": "UniRef50_P47582", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47582" ], "product": "DNA-directed RNA polymerase subunit beta'" } }, "id": "KMADJIBOEG_794", "locus": "GCA000292445_00790" }, { "type": "cds", "sequence": "contig_1", "start": 433817, "stop": 433915, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAACAACAAGACGTAATAAAAGAAATAACAAGCTTTATAAAAACATTAAAGCAATTAAACTTTCCATCGCTTCCAATGACACCATTTTGAACTGA", "aa": "MTTTRRNKRNNKLYKNIKAIKLSIASNDTILN", "aa_hexdigest": "05503160a2613d897970a8709258a958", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3734.3353000000006, "isoelectric_point": 11.165940284729004 }, "id": "KMADJIBOEG_795", "locus": "GCA000292445_00791" }, { "type": "cds", "sequence": "contig_1", "start": 433925, "stop": 434194, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04560.25" ], "nt": "TTGCAAGAACTTTTAACCATTAAATCTGATGATGTACAAGGAAGAAATAGGGCTTATGCTGCTATTGTTAAAGGTGCAGCTTTCCCAGAGCCTGGTATCCCTGAATCATTTAAATTATTGACAAAAGAATTACAGGGCTTGGCTTTATCTGTTTCATTTATCTATGATGACAACACCCAACAAGACTCCAATAATGTTTCCATCTTGCAAAGTGATGGGGAACAAGATGAATTTTTCAATGATTTTGAATTTGACACTGAGGGTTATTAG", "aa": "MQELLTIKSDDVQGRNRAYAAIVKGAAFPEPGIPESFKLLTKELQGLALSVSFIYDDNTQQDSNNVSILQSDGEQDEFFNDFEFDTEGY", "aa_hexdigest": "7ad708e8f50a0819af35d6c2caab9c68", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04560.25", "name": "RNA_pol_Rpb2_7", "length": 64, "aa_cov": 0.6067415730337079, "hmm_cov": 1.0, "evalue": 3.612316001571592e-13, "score": 44.94830322265625, "start": 1, "stop": 54 } ], "seq_stats": { "molecular_weight": 9971.774399999997, "isoelectric_point": 4.0500284194946286 }, "id": "KMADJIBOEG_796", "locus": "GCA000292445_00792" }, { "type": "cds", "sequence": "contig_1", "start": 434225, "stop": 435301, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00562.33" ], "nt": "TTGTCTACTAAAAATGGTGATGAACAAATTACCCGTGATATCCCTAATGTTAGTGATGCAAACAAACGCTATCTTGATGAGAATGGCATCATTATGGTGGGTGCTGAAGTTAAAGAAGGGGATGTTTTGGTTGGTAAAGTTTCCCCTAAAGGTCAAGTCGAAGTCTCTCCTGAAGAAAAGCTATTTAAAGCCATCTTCCCTGAAAGTGTTCAAAACGTGAGAGACTCTTCACTTAAAGTTCCCCATGGTGGGGATGGTATTGTTTCAGCTGTAAAACGTTTTTCAATTGCTAATGGTGATGAACTTAATGATGGTGTGATTGAAATGATCAAGGTTTATGTGGTTCAAAAACGTAAGATTCAAATTGGTGATAAATTAGCTGGTAGACACGGAAATAAAGGAGTTATTTCTAAAGTGGTGCCTATTGAAGATATGCCCCATTTAGAAGATGGAACCCCAGTTGATATTCTACTCAACCCCCTTGGTGTTCCTAGTCGGATGAACATAGGACAAATTTTTGAAACCCACTTGGGTTATGCAGCACACAAGCTAGCAGTTCGTTCTTTAATTAGTAGTTGTTTTGATCAAAATAAAGCTAAGGAGTTTGCCATTGAAATCAATCAACCTCAAGCAAGGGTTGAAAGATTAATTAAAGGTTTAAAAAGCCAAATCAATGATCGCAATATTAAAAGTGAAAAAGAAGCACTTGAAAAACTCGATAACAGTGACATTAGTTTAGTTTTGAAAGAGATAGGGATGTCTTTTGATGATCTTATTTACAAAATTGCAACCCCTATTTTCCAAGGAGTGAACTTCTTAGATCTCCAAGATGTTATGCAAGAAGCAGGATTAGATCCCCAAAAAAATCAGGGTAAGTTTAAACTCATTGATGGTAGGAGTGGAATGCCATTTGAAAGACCTATTTCACTTGGAATTATGTACATGATGAAGCTGAATCACATGGTTGATGATAAGATCCATGCTCGTGCTGTTGGCCCTTATTCTAAGATCACCCAACAACCATTAGGTGGTAAATCGCAAAATGGTGGTCAGCGGTTTGGTGAGATGGAAGTGTGA", "aa": "MSTKNGDEQITRDIPNVSDANKRYLDENGIIMVGAEVKEGDVLVGKVSPKGQVEVSPEEKLFKAIFPESVQNVRDSSLKVPHGGDGIVSAVKRFSIANGDELNDGVIEMIKVYVVQKRKIQIGDKLAGRHGNKGVISKVVPIEDMPHLEDGTPVDILLNPLGVPSRMNIGQIFETHLGYAAHKLAVRSLISSCFDQNKAKEFAIEINQPQARVERLIKGLKSQINDRNIKSEKEALEKLDNSDISLVLKEIGMSFDDLIYKIATPIFQGVNFLDLQDVMQEAGLDPQKNQGKFKLIDGRSGMPFERPISLGIMYMMKLNHMVDDKIHARAVGPYSKITQQPLGGKSQNGGQRFGEMEV", "aa_hexdigest": "b0d0200ffa32cc819bd5fe1be07362ea", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00562.33", "name": "RNA_pol_Rpb2_6", "length": 351, "aa_cov": 0.9636871508379888, "hmm_cov": 0.7435897435897436, "evalue": 2.960577939917215e-95, "score": 314.5927429199219, "start": 3, "stop": 347 } ], "seq_stats": { "molecular_weight": 39556.035700000044, "isoelectric_point": 6.421294212341309 }, "id": "KMADJIBOEG_797", "locus": "GCA000292445_00793" }, { "type": "cds", "sequence": "contig_1", "start": 435401, "stop": 438097, "strand": "-", "frame": 3, "gene": "rpoB", "product": "DNA-directed RNA polymerase subunit beta", "db_xrefs": [ "COG:COG0085", "COG:K", "EC:2.7.7.6", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549", "KEGG:K03043", "SO:0001217", "UniRef:UniRef50_P47715", "UniRef:UniRef90_P47583" ], "nt": "ATGTCACAAAAATCTAATTTCTTTCAAAAACGTTATTCCCCTACAGCTACCAGAAGGTATTATGGCAAAATTGAGACCAATTTTATCCAACCAAATTTAGCTGATATTCAGATTAAAAGCTACCAAAAATTCTTAGATCATGATCTTGAAAAATTAATTGCCTCATATTTTCCAATCAAATCCCCTAATGATCGCTACACTATCAATTTTAGGGGATTACACAGAACTGAACCAGAACGTGATGAAGCACAATCACGTGCTCAATCTAAAACTTATGAAGTTGGTATTTATGCTGATCTTGAATTAGTTGATAATGATAAAGGAACAGTTAAAAAAGCACGGAAATCAAAGAAAAATATTGCTAGCAATACAAATGGTGTATTTTTAGCTAACATGCCTTTAATAACCCATGATGGGGTTTTTATTATCAATGGGATAGAAAAGTTTGTTATTTCCCAAATAACCCGTTCTCCAGGGATATACATGCTAACAAAATCCCAACTAAAACTATCCAACTCCCGTAAAAGAGTACAGGAAGGTTATGTTTGTGAGGTTTTACCTGCTAATGGTTCAGTGATGCTCATATACATCTCCAATAAAAAAAAGATTGAAGATGCTTTTGTACAAATTCTTTTAAGAGATGCGGTAAGAGAAGGTGCTAAGATTTTCCCTATTACAACACTTTTAAAAGCGTTTGGTTTAAATAATCGTGAGATCCTTAAAATCTTTAAAAACAATGAATTTATCAAACGTTCATTGGAAGCGGAAATTTACAATGCTAAGGATTTTTTAAGCAATGTTGATCCTGAAATCAAAAACCTTTTAAAAGAATTTAGAGATGGTAAAACTGATTTAAGAAGAAAAGGGATCGCTTCTGATCAGAAATTAAGATCACTTGTTAATGAATATGTAACGCTTGAGAAACAATATAATGCGCTAAAACAAACAAGTCCAAATGATTCTAGTTTAACTGCACTTGAACTGGAAATGGAAAACAAAATGGATAGTGTTATTACTGAAAGAGCTGCTAAACACATTGTCAATGAACTTTCTATTTCACTGCGTGATATTGAAAACACTGAAGAGTGTCATGAAGTGAGTTTCCATGCACTTTTATGTGCGCGTTTTTTTAGAAACAAGAGGTACAACCTCTCTAATGCAGGGAGATACAAAGTATCTAGAAAGTTACGTTTAACAGAACGTATTTATCAAAAAACTCTGGCATGTGATTTGTTCTTAAAAGATGGCAAGCTACTTTTAAAAAAAGGTACTTTACTTTTAAAAGAAGAGATTGACAAAATCAAACAAGCTGCTAAGAACAATGAAATTAGTTTTGTTAATAAAATGCAACTTACAACTGATGGTAAGGCTGTTGATTTAGCAAAAGAATCACTCTTTTATGAAACGATAGATGTATATATCACTAATGATAATCTTAGTGTTTCAGTACCAGTTATAGGGATCCATAACGAAAATGATCTGAACAAAGCAATGACTCTCAGTGATTTCATCGCTTCAATTAGTTATGTGATTAATTTACCTTATGGAATTGGTAAATATGATGATATTGATCACCTTGGTAATAAGCGGGTTAAGTTAATTAATGAATTAATTACTGCTAAATTAGAAAGCGGCTTTACTAGAATGGAGCGCTTTTTAAAAGAAAAGTTAACTATTGCTGATGGAGTTAACCGTGGCCAGCAAATTAATGAAGAGGGTCAGGTTATTGAACAAGGTGAAAAAAAGGAATTAACTATTAAATCTTTAATCAACTCAAAACCAATTCAAATTGTGATTAAAGACTTCTTCAATACCCACCAATTAACCCAATTTTTAGACCACCAAAACCCTTTATCAGAATTGAGTAATAAAAGAAGAATTTCAGCAATGGGACCTGGGGGAATATCAAGAGAGGACCCTAATTTAGATATCCGTGATGTGCATTATTCTCAGTACGGTAGAATTTGCCCTATTGAAACACCTGAAGGGATGAACATAGGGTTGATCATGTCTTTAGCTAGCTTTGCTAAGATTGATGAAAACGGATTTTTAATGGCACCTTATCGCAAAATCAAAGCTGGGGTAATTACTGATGAGGTGGAATATTTAACTGCGCTTAGAGAAGATGAACATATTATTGCTGAGATCTCTTCACTTGTCAATATTAGTAACGATAACAAGATCTTAGATAAGGAAATTATTGGTAGGTATCGATCTATGCAAGGACTTTATGATCCTTTAAAGATTGATTACATTGATGTAGCACCACACCAAGTTGTTTCCATTGGTTCTTCTTTAATCCCCTTTTTGGAAAATGATGATTCAGCTAGAGCATTAATGGGAACCAACATGCAACGTCAGGCCTATCCTTTAATAAAGCCATATGCTCCTGCAGTAGGTACTGGTCAAGAACACAAAATTGCTAGTGATTCAGGTTTAACAATGTCCTCTCCTTGCTCAGGTGTTGTTAGTTATGTTGATAACAGTAAGATTATTATTACAAGTGATAGTTCTAAAAAAGAGACAGTTAACCTAGTTAAATTTGAACGTTCCAACCAAAATACTTGTTATAACCACAAACCAATTGTTGAAATAGGCCAAAGGGTTAATAAGGATGAAATCATTGTTGATGGCCCTGCTGTTAATAAGAGTGAGTTGGCATTAGGACAGAATGTTTTAGTTGCTTTTACAACTTGA", "aa": "MSQKSNFFQKRYSPTATRRYYGKIETNFIQPNLADIQIKSYQKFLDHDLEKLIASYFPIKSPNDRYTINFRGLHRTEPERDEAQSRAQSKTYEVGIYADLELVDNDKGTVKKARKSKKNIASNTNGVFLANMPLITHDGVFIINGIEKFVISQITRSPGIYMLTKSQLKLSNSRKRVQEGYVCEVLPANGSVMLIYISNKKKIEDAFVQILLRDAVREGAKIFPITTLLKAFGLNNREILKIFKNNEFIKRSLEAEIYNAKDFLSNVDPEIKNLLKEFRDGKTDLRRKGIASDQKLRSLVNEYVTLEKQYNALKQTSPNDSSLTALELEMENKMDSVITERAAKHIVNELSISLRDIENTEECHEVSFHALLCARFFRNKRYNLSNAGRYKVSRKLRLTERIYQKTLACDLFLKDGKLLLKKGTLLLKEEIDKIKQAAKNNEISFVNKMQLTTDGKAVDLAKESLFYETIDVYITNDNLSVSVPVIGIHNENDLNKAMTLSDFIASISYVINLPYGIGKYDDIDHLGNKRVKLINELITAKLESGFTRMERFLKEKLTIADGVNRGQQINEEGQVIEQGEKKELTIKSLINSKPIQIVIKDFFNTHQLTQFLDHQNPLSELSNKRRISAMGPGGISREDPNLDIRDVHYSQYGRICPIETPEGMNIGLIMSLASFAKIDENGFLMAPYRKIKAGVITDEVEYLTALREDEHIIAEISSLVNISNDNKILDKEIIGRYRSMQGLYDPLKIDYIDVAPHQVVSIGSSLIPFLENDDSARALMGTNMQRQAYPLIKPYAPAVGTGQEHKIASDSGLTMSSPCSGVVSYVDNSKIIITSDSSKKETVNLVKFERSNQNTCYNHKPIVEIGQRVNKDEIIVDGPAVNKSELALGQNVLVAFTT", "aa_hexdigest": "cf4db95a6cd8473f08a6ad3ca80c4fc2", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpoB" ], "truncated": "5-prime", "pseudogene": { "start": 435098, "stop": 438097, "observations": { "mutation": [ 435403 ], "stop": [ 435403 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47583", "query_cov": 1.0, "subject_cov": 0.6460431654676259, "identity": 0.9990000000000001, "score": 1719.0, "evalue": 0.0, "gene_start": 1, "gene_end": 898, "reference_start": 1, "reference_end": 898, "reference_sequence": "MSQKSNFFQKRYSPTATRRYYGKIETNFIQPNLADIQIKSYQKFLDHDLEKLIASYFPIKSPNDRYTINFRGLHRTEPERDEAQSRAQSKTYEVGIYADLELVDNDKGTVKKARKSKKNIASNTNGVFLASMPLITHDGVFIINGIEKFVISQITRSPGIYMLTKSQLKLSNSRKRVQEGYVCEVLPANGSVMLIYISNKKKIEDAFVQILLRDAVREGAKIFPITTLLKAFGLNNREILKIFKNNEFIKRSLEAEIYNAKDFLSNVDPEIKNLLKEFRDGKTDLRRKGIASDQKLRSLVNEYVTLEKQYNALKQTSPNDSSLTALELEMENKMDSVITERAAKHIVNELSISLRDIENTEECHEVSFHALLCARFFRNKRYNLSNAGRYKVSRKLRLTERIYQKTLACDLFLKDGKLLLKKGTLLLKEEIDKIKQAAKNNEISFVNKMQLTTDGKAVDLAKESLFYETIDVYITNDNLSVSVPVIGIHNENDLNKAMTLSDFIASISYVINLPYGIGKYDDIDHLGNKRVKLINELITAKLESGFTRMERFLKEKLTIADGVNRGQQINEEGQVIEQGEKKELTIKSLINSKPIQIVIKDFFNTHQLTQFLDHQNPLSELSNKRRISAMGPGGISREDPNLDIRDVHYSQYGRICPIETPEGMNIGLIMSLASFAKIDENGFLMAPYRKIKAGVITDEVEYLTALREDEHIIAEISSLVNISNDNKILDKEIIGRYRSMQGLYDPLKIDYIDVAPHQVVSIGSSLIPFLENDDSARALMGTNMQRQAYPLIKPYAPAVGTGQEHKIASDSGLTMSSPCSGVVSYVDNSKIIITSDSSKKETVNLVKFERSNQNTCYNHKPIVEIGQRVNKDEIIVDGPAVNKSELALGQNVLVAFTTWNGYNYEDAIVISERLVKEDILTSLTINEYVAQCLSTKNGDEQITRDIPNVSDANKRYLDENGIIMVGAEVKEGDVLVGKVSPKGQVEVSPEEKLFKAIFPESVQNVRDSSLKVSHGGDGIVSAVKRFSIANGDELNDGVIEMIKVYVVQKRKIQIGDKLAGRHGNKGVISKVVPIEDMPHLEDGTPVDILLNPLGVPSRMNIGQIFETHLGYAAHKLAVRSLISSCFDQNKAKEFAIEINQPQARVERLIKGLKNQINDRNIKSEKEALEKLDNSDISLVLKEIGMSFDDLIYKIATPIFQGVNFLDLQDVMQEAGLDPQKNQGKFKLIDGRSGMPFERPISLGIMYMMKLNHMVDDKIHARAVGPYSKITQQPLGGKSQNGGQRFGEMEVWALEAYGAAYNLQELLTIKSDDVQGRNRAYAAIVKGAAFPEPGIPESFKLLTKELQGLALSVSFIYDDNTQQDSNNVSILQSDGEQDEFFNDFEFDTEGY" }, "paralog": false, "identity": 0.997997997997998, "score": 1930.0, "evalue": 0.0, "description": "Internal stop codon at 435403. Nonsense mutation around 435403.", "psc": { "uniref90_id": "UniRef90_P47583", "gene": "rpoB", "product": "DNA-directed RNA polymerase subunit beta", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P47715", "cog_id": "COG0085", "cog_category": "K", "kegg_orthology_id": "K03043", "go_ids": [ "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47583", "EC:2.7.7.6", "UniRef:UniRef50_P47715", "COG:COG0085", "COG:K", "KEGG:K03043", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549" ] }, "pscc": { "uniref50_id": "UniRef50_P47715", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47715" ], "product": "DNA-directed RNA polymerase subunit beta" } }, "id": "KMADJIBOEG_798", "locus": "GCA000292445_00794" }, { "type": "cds", "sequence": "contig_1", "start": 438187, "stop": 438693, "strand": "-", "frame": 1, "gene": "ssuE", "product": "putative protein MG342", "db_xrefs": [ "COG:C", "COG:COG0431", "GO:0005524", "GO:0005829", "GO:0010181", "GO:0016491", "RefSeq:WP_014894303.1", "SO:0001217", "UniParc:UPI00027B4078", "UniRef:UniRef100_UPI00027B4078", "UniRef:UniRef50_P75270", "UniRef:UniRef90_P47584" ], "nt": "ATGTCAAAAGATCAAAAGTCATTAATTTTAATGCTCTCCAATTCGCAACATTCCATTAACAGGAAATTTGCTAATGAATTAAAACATTCTTTAAGTTGTGAATTGATTGAGTTAAAAGACTATCAAGTTGATTTTTATTCTGTTGATTTAGAAGCAACTAACTTTCCTGACAAAATTAAAACTTTAGTTAGAAAAATCAAAGAACATAGCAATTTAATTTTTGTTACCCCTGAACACAACGGTTTTATCCCTGCTTTTGCTAAAAACATCATCGACTGGATGACTCGTGATGAGCAGTATGGTAGAAATCAATTTTTAAAAGGTCTTAATGGAGTTATTTGTTGTGTAACTCCAGCAACTGCGGGTGGGGGTAAAACTGTTCTAGAATTACTAGATAAATTTTTAAGTTTTAGCGCTCTTAATGTTAAAGGAACAGTTTTAGTCAACGGTTATCATGAGAACTTTGATTTTAAGCCTTTTATTGAACAAGTTAAAAAATTAATATAA", "aa": "MSKDQKSLILMLSNSQHSINRKFANELKHSLSCELIELKDYQVDFYSVDLEATNFPDKIKTLVRKIKEHSNLIFVTPEHNGFIPAFAKNIIDWMTRDEQYGRNQFLKGLNGVICCVTPATAGGGKTVLELLDKFLSFSALNVKGTVLVNGYHENFDFKPFIEQVKKLI", "aa_hexdigest": "9bea3d93f331b107a3b8ac86e41a7518", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B4078", "ncbi_nrp_id": "WP_014894303.1", "uniref100_id": "UniRef100_UPI00027B4078", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B4078", "RefSeq:WP_014894303.1", "UniRef:UniRef100_UPI00027B4078" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B4078", "uniref90_id": "UniRef90_P47584" }, "psc": { "uniref90_id": "UniRef90_P47584", "gene": "SsuE", "product": "Uncharacterized protein MG342", "uniref50_id": "UniRef50_P75270", "cog_id": "COG0431", "cog_category": "C", "go_ids": [ "GO:0005829", "GO:0005524", "GO:0010181", "GO:0016491" ] }, "pscc": { "uniref50_id": "UniRef50_P75270", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75270" ], "product": "Uncharacterized protein MG342 homolog" }, "genes": [ "ssuE" ], "id": "KMADJIBOEG_799", "locus": "GCA000292445_00795" }, { "type": "cds", "sequence": "contig_1", "start": 438772, "stop": 438954, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTTTGATTTGGAATTCCACTTAATTGTTGAAATAATGAAAGGTTTTAATTTTGATGAGAATTGTATCTGTTTACTTGAAGGAAATAAAGAACAAAACCCCAATTTACCAACCGTTAGTTTAGATACTAAGGTTGCTGATATTAAGACTTATCAAGGTTGCTATTTTCTCAATAATTAA", "aa": "MIFDLEFHLIVEIMKGFNFDENCICLLEGNKEQNPNLPTVSLDTKVADIKTYQGCYFLNN", "aa_hexdigest": "c9af8b3911cbb767f1463288a6edf938", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6944.911500000004, "isoelectric_point": 4.395041084289551 }, "id": "KMADJIBOEG_800", "locus": "GCA000292445_00796" }, { "type": "cds", "sequence": "contig_1", "start": 439051, "stop": 439446, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAATAGTTTTTTTATTAATTAATTCACCAGAAGAAAAAACAACTATTAAGCTTTCTGATTCGCTAATTAAAGAAATTAAAAAACAAAAAATAAAACATGAAGTTTATTCTTTAAGATCATTTCAAAATAAGTTTTTTAAGTTAGTTAGAAAATTAGAAAAAAAGCACAAGTCTAAAAATAACGTAATGTTTGTTGAACTAAACGGTGTGTTTGGTTATGAAACTAACTTTGAAAAAAGTAACATTGATTTTAGTTTCACTAGTTTTCAAGACAGATTCACAATTGAAAAAAAGTTAAAAATCACCAGCCATTTTATCTTGCCTCATAAATACTTGTTAAAAAATTTAGAAAACATTAAGCATCCTAACTTTGAGCAAAAGAACTTAATTTGA", "aa": "MKIVFLLINSPEEKTTIKLSDSLIKEIKKQKIKHEVYSLRSFQNKFFKLVRKLEKKHKSKNNVMFVELNGVFGYETNFEKSNIDFSFTSFQDRFTIEKKLKITSHFILPHKYLLKNLENIKHPNFEQKNLI", "aa_hexdigest": "6cf12a82c5c16982bc8a65fc2ac0d402", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15738.292599999992, "isoelectric_point": 9.8639368057251 }, "id": "KMADJIBOEG_801", "locus": "GCA000292445_00797" }, { "type": "cds", "sequence": "contig_1", "start": 439573, "stop": 439812, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTCATAAAATTAATGATCAAACCCTCGTTAATGAGCAACGCCTATTAGCTTATGATTTTTTTCAAAATAGCAATAAGGTTGGACTTTTATCAACATTACAATATCTAGATTTTGTTAATTTTTTAGTTAGATCTAAAAAAGTAAATTATCTGCTTGTTAACAAAGTAAGTCTTCCAATATATCAAAAGATTTATTTTGATAACTTCCCTTTTTTAGGTTTTGATAATAACTTGTGA", "aa": "MSHKINDQTLVNEQRLLAYDFFQNSNKVGLLSTLQYLDFVNFLVRSKKVNYLLVNKVSLPIYQKIYFDNFPFLGFDNNL", "aa_hexdigest": "cfd8b67c0df74949332d557a25b5fc2e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9368.678999999995, "isoelectric_point": 9.00418643951416 }, "id": "KMADJIBOEG_802", "locus": "GCA000292445_00798" }, { "type": "cds", "sequence": "contig_1", "start": 439816, "stop": 440100, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAAAAGCTTTCTAATAATATTTTGAAAGAACTACCAGTTTTAAATAAGACTTATAAAAATTTAAAGTTACCAGTGTTTTTGGTTTTAGCTCAAAATGATTTATTTATGCCAACAAAATTAACATTAAGCTATTTCAATAAATACCTAATTAAGAACAATAATTTGCAATCATCTGTTATCTTAAACAGTGAACACCAAATGTTTAATAGTAAATATGAAAGCTTTTGTAAAGCAATGGATGATATCCTGAATCATAATAAACTAAGTAAAATTTATTAA", "aa": "MAKKLSNNILKELPVLNKTYKNLKLPVFLVLAQNDLFMPTKLTLSYFNKYLIKNNNLQSSVILNSEHQMFNSKYESFCKAMDDILNHNKLSKIY", "aa_hexdigest": "9eedfe8da8d8eb0270fad1306074968c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11015.864299999994, "isoelectric_point": 9.608835411071777 }, "id": "KMADJIBOEG_803", "locus": "GCA000292445_00799" }, { "type": "cds", "sequence": "contig_1", "start": 440128, "stop": 440337, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGCTGCTGTTTGTAGTTATGCCATGAATTTATTAAATGCTAAACGTGTTGAAAAACTCATACTTTTAGCACCTCTTTCATACTGTAACTTATTGAGATATTTCAAAATCAAAAGTTCTTTTAAAAAAGATAAGGCAGAAAGAATGGCTAATTTTAAAGCAATGTTTCAAACTAAATTTAGTAATTTAACTGATGAAAACAGCTGA", "aa": "MGAAVCSYAMNLLNAKRVEKLILLAPLSYCNLLRYFKIKSSFKKDKAERMANFKAMFQTKFSNLTDENS", "aa_hexdigest": "53fff15810565e49c23b518d71d95a70", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7917.299999999997, "isoelectric_point": 9.745379447937012 }, "id": "KMADJIBOEG_804", "locus": "GCA000292445_00800" }, { "type": "cds", "sequence": "contig_1", "start": 440802, "stop": 441017, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAACCTCTTTACAAAACCGACTGTTTTTAAAGTTGCTAACTCTAAGAATTTAGAAAATATCTATAAAACTTTTACTAGTATTAAAAATGCTGCTTTCAAAGAAATTGAAAAGCTAAGAAAAGAAGGGTTGATTTCTAAAAATAATCAAATTGAATTAACCGTTGGAATTAATAAAAAAATACCCAAAAAATTAAAGGATAATCTCGCACTTTGA", "aa": "MNLFTKPTVFKVANSKNLENIYKTFTSIKNAAFKEIEKLRKEGLISKNNQIELTVGINKKIPKKLKDNLAL", "aa_hexdigest": "68d5682c9058b674823a24a2206c3b2e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8121.540599999993, "isoelectric_point": 10.056761741638184 }, "id": "KMADJIBOEG_805", "locus": "GCA000292445_00801" }, { "type": "cds", "sequence": "contig_1", "start": 441210, "stop": 441347, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGATTATAAGTTTTCACTAGAAGACAAAATAGTTATCCATAAAACTAATTCACTAGTAGAACAAATTGAGAAATTTTTAGAGAAATATAATTTTTTAGGTTGCCTAAAAGTGATTAATAAGTTTGTTTTATGA", "aa": "MDDYKFSLEDKIVIHKTNSLVEQIEKFLEKYNFLGCLKVINKFVL", "aa_hexdigest": "6c768a02f337ced0c35d4b18980563ae", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5394.306199999999, "isoelectric_point": 6.534574317932129 }, "id": "KMADJIBOEG_806", "locus": "GCA000292445_00802" }, { "type": "cds", "sequence": "contig_1", "start": 441486, "stop": 441731, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "TTGGAAATAAATAAATATGGTTCAATAGCTGATCTTTATATTGAAGGTTCTGATCAATATCGGGGTTGGTTCAACTCTTCTTCAAATTGCGGAATTATTCAAAATGATTTAATCCCTTTTAAATCACTTGTTTCACATGGTTTTACACTTGATGAAAATGGCAATAAAATGTCAAAGTCATTAGGAAACATAGTTGATCCTTTAAAAATTTGTGATCAATATGGAGCGGATATATTAAGGTTGTGA", "aa": "MEINKYGSIADLYIEGSDQYRGWFNSSSNCGIIQNDLIPFKSLVSHGFTLDENGNKMSKSLGNIVDPLKICDQYGADILRL", "aa_hexdigest": "4bb8445ed36ea9a405cc1e4836f8a11b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 72, "aa_cov": 0.8888888888888888, "hmm_cov": 1.0, "evalue": 1.1871089945199035e-26, "score": 88.53526306152344, "start": 10, "stop": 81 } ], "seq_stats": { "molecular_weight": 9033.086399999993, "isoelectric_point": 4.791606712341309 }, "id": "KMADJIBOEG_807", "locus": "GCA000292445_00803" }, { "type": "cds", "sequence": "contig_1", "start": 442137, "stop": 442409, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAATACATGTTGTTGATAATGAGGGAACAGGTATTGTTCACAGCTCCCCTGCATTTGGAATTGATGATTTTTATCTTTGTCAAAAAAACAAGATTAAAGAAGTTTTGATTTCTATTGATGAGAAAGGTGTATTTAATAACTTACTTAATGATAAAGAACTTGAGAATTGTTTTTATCTTAAAGCAAATGATCTAATTATTAATCGTTTAAAACAAAACAATAGCTTTATTTTTTCTGAAGTTATTTCCCACCGCGAACCACATGATTGA", "aa": "MGIHVVDNEGTGIVHSSPAFGIDDFYLCQKNKIKEVLISIDEKGVFNNLLNDKELENCFYLKANDLIINRLKQNNSFIFSEVISHREPHD", "aa_hexdigest": "6224d419f170cbc0dc5f76aa7e67cbae", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10306.548399999989, "isoelectric_point": 5.225913429260252 }, "id": "KMADJIBOEG_808", "locus": "GCA000292445_00804" }, { "type": "cds", "sequence": "contig_1", "start": 442776, "stop": 443141, "strand": "-", "frame": 2, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0029621904", "UniRef:UniRef90_UPI0029621904" ], "nt": "GTGGGACATGCTCTTAACAAGATTTTAAAAGACTTCATTTTACGTAGTTGGTTATATGAAGGATATGATGTTGTTTTTATTCCTGGTTGGGATTGTCATGGACTACCAATAGAACATGCAGTTAGTAAGAAAAACCCTAGTAGTTATAGCAATCTTTCAACTGTTGAAAAAAGAAAATTATGTCATCAGTTTGCACTTTCACAAATTGCAGTTCAAAAAGAACAATTTCAAAGACTGGGACTTTTAAATGATTTTCAAAACTGTTATTACACAATAGATGAGAGTTTTCAATTTAAGGAACTTGAACTATTTTTACAAGCAATTAAAAAAGGGCTCATTTTTCAAGATTTAAAACCAACTTATTGA", "aa": "MGHALNKILKDFILRSWLYEGYDVVFIPGWDCHGLPIEHAVSKKNPSSYSNLSTVEKRKLCHQFALSQIAVQKEQFQRLGLLNDFQNCYYTIDESFQFKELELFLQAIKKGLIFQDLKPTY", "aa_hexdigest": "7e4deceb4c8eb1dcf961f4576070d4c5", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 442735, "stop": 443189, "observations": { "deletion": [ 442814 ], "stop": [ 442748 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0029621904", "query_cov": 0.9752066115702479, "subject_cov": 0.7712418300653595, "identity": 0.941, "score": 230.0, "evalue": 6.68e-76, "gene_start": 1, "gene_end": 118, "reference_start": 17, "reference_end": 134, "reference_sequence": "MKILHDGPPYANGSIHVGHALNKILKDFILRSWLYEGYDVVFIPGWDCHGLPIEHAVSKKNPSSYSNLSTVEKRKLCHQFALSQIAVQKEQFQRLGLLNDFQNCYYTIDESFQFKELELFLQAIKKRAHFSRFKTNLLITNFKNFTCWSGNWI" }, "paralog": false, "identity": 0.9867549668874173, "score": 306.0, "evalue": 2.56e-108, "description": "Internal stop codon at 442748. Frameshift due to deletion around 442814.", "psc": { "uniref90_id": "UniRef90_UPI0029621904", "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_UPI0029621904", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0029621904", "UniRef:UniRef50_UPI0029621904" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0029621904", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0029621904" ], "product": "class I tRNA ligase family protein" } }, "id": "KMADJIBOEG_809", "locus": "GCA000292445_00805" }, { "type": "cds", "sequence": "contig_1", "start": 443377, "stop": 443544, "strand": "+", "frame": 1, "gene": "spoU", "product": "tRNA/rRNA methyltransferase SpoU type domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A917CEE2" ], "nt": "ATGTATAAATCAGTAATAAACATAGTTTTATTTTGTCCAGAAATTCCTAATAACACTGGCAACATCGTACGTAGTTGCACTGCTTTTAAAGCTAATCTACACTTAATTAAACCTTATGGCTTTTTCTTAAATGATAAAAGGATGGTTAGAGCTGGTTTAAATTGTTGA", "aa": "MYKSVINIVLFCPEIPNNTGNIVRSCTAFKANLHLIKPYGFFLNDKRMVRAGLNC", "aa_hexdigest": "e695ddec9a2106217f086fb914ef11b9", "start_type": "ATG", "rbs_motif": "ATTTA", "psc": { "uniref90_id": "UniRef90_UPI0023B9359B", "query_cov": 0.9090909090909091, "subject_cov": 0.819672131147541, "identity": 0.62, "score": 65.9, "evalue": 3.47e-13, "valid": false, "product": "tRNA (cytidine(34)-2'-O)-methyltransferase", "ec_ids": [ "2.1.1.207" ], "uniref50_id": "UniRef50_A0A917CEE2", "kegg_orthology_id": "K03216" }, "pscc": { "uniref50_id": "UniRef50_A0A917CEE2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A917CEE2" ], "product": "tRNA/rRNA methyltransferase SpoU type domain-containing protein" }, "genes": [ "spoU" ], "id": "KMADJIBOEG_810", "locus": "GCA000292445_00806" }, { "type": "cds", "sequence": "contig_1", "start": 443662, "stop": 443775, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAATAAATTACCAAACGAACTTTACTTTGTTTTTGGTCAGGAAACAAAGGGATTACCTAAAACAATCATGGATAACTTTAAACAAAACCAAATTAGAATTCCCATTTGA", "aa": "MTNKLPNELYFVFGQETKGLPKTIMDNFKQNQIRIPI", "aa_hexdigest": "b71224ab4b6b21218cc93ad966e91af1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4368.0843, "isoelectric_point": 9.398732948303223 }, "id": "KMADJIBOEG_811", "locus": "GCA000292445_00807" }, { "type": "cds", "sequence": "contig_1", "start": 443868, "stop": 444209, "strand": "+", "frame": 3, "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "db_xrefs": [ "COG:COG0220", "COG:J", "EC:2.1.1.33", "GO:0006400", "GO:0008176", "GO:0030488", "GO:0036265", "GO:0043527", "SO:0001217", "UniRef:UniRef50_P47589", "UniRef:UniRef90_P47589" ], "nt": "GTGCGCTTAAGAAAAGTTAAAAACGCTCTTTTAAAAATTAATCAAAGTCCTTATTTTTATTCAAAAGATAAGTTTGCTAAGTTTACTAAAAAACAATTAGTGCTGGAATTGGGTTGTGGTAAGGGTACTTTTTTAATCAAAGAAGCACAAAAAAATAACAATTTTCTTTTTATAGGAATTGAACGTGAACCTACAATTGTTTTAAAAGCAATTAACAAAATTAACAAGTTGGATTTTAATTTGGAAAATATCTTATTGTTGTGTACAGATGCAAAACAACTTGATGATTATTTTCAAGCTGAATCTGTTCAAAAAATCTTTATTAATTTCCCTGATCCTTGA", "aa": "MRLRKVKNALLKINQSPYFYSKDKFAKFTKKQLVLELGCGKGTFLIKEAQKNNNFLFIGIEREPTIVLKAINKINKLDFNLENILLLCTDAKQLDDYFQAESVQKIFINFPDP", "aa_hexdigest": "ebf3cc8848cdb8fad7990322f4f92b93", "start_type": "GTG", "rbs_motif": null, "genes": [ "trmB" ], "truncated": "3-prime", "pseudogene": { "start": 443868, "stop": 444500, "observations": { "mutation": [ 444207, 444267 ], "stop": [ 444207, 444267 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47589", "query_cov": 1.0, "subject_cov": 0.5380952380952381, "identity": 1.0, "score": 224.0, "evalue": 4.29e-73, "gene_start": 1, "gene_end": 113, "reference_start": 1, "reference_end": 113, "reference_sequence": "MRLRKVKNALLKINQSPYFYSKDKFAKFTKKQLVLELGCGKGTFLIKEAQKNNNFLFIGIEREPTIVLKAINKINKLDFNLENILLLCTDAKQLDDYFQAESVQKIFINFPDPWPKKRHIQRRLTSPDFLKLFWNLLVKNGLIEFKTDNDKLFEYTLTTLQENSQIFEIIHQITDLNNSEFSFQNSITEYEQRFMELEIPIKKLVIKKII" }, "paralog": false, "identity": 0.9857142857142858, "score": 407.0, "evalue": 9.83e-148, "description": "Internal stop codon at 444207, 444267. Nonsense mutation around 444207, 444267.", "psc": { "uniref90_id": "UniRef90_P47589", "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "ec_ids": [ "2.1.1.33" ], "uniref50_id": "UniRef50_P47589", "cog_id": "COG0220", "cog_category": "J", "go_ids": [ "GO:0006400", "GO:0008176", "GO:0030488", "GO:0036265", "GO:0043527" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47589", "EC:2.1.1.33", "UniRef:UniRef50_P47589", "COG:COG0220", "COG:J", "GO:0006400", "GO:0008176", "GO:0030488", "GO:0036265", "GO:0043527" ] }, "pscc": { "uniref50_id": "UniRef50_P47589", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47589" ], "product": "tRNA (guanine-N(7)-)-methyltransferase" } }, "id": "KMADJIBOEG_812", "locus": "GCA000292445_00808" }, { "type": "cds", "sequence": "contig_1", "start": 444345, "stop": 444500, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGAAAATAGTCAAATTTTTGAAATTATCCATCAAATAACTGATCTTAACAATTCTGAATTCAGTTTTCAAAATAGTATTACTGAATATGAACAGCGCTTTATGGAATTAGAAATTCCAATTAAAAAACTAGTGATTAAGAAAATAATTTAA", "aa": "MQENSQIFEIIHQITDLNNSEFSFQNSITEYEQRFMELEIPIKKLVIKKII", "aa_hexdigest": "7a662a05397a943d6bd9c36add1aa56d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6172.043699999997, "isoelectric_point": 4.876922035217285 }, "id": "KMADJIBOEG_813", "locus": "GCA000292445_00809" }, { "type": "tRNA", "sequence": "contig_1", "start": 444533, "stop": 444608, "strand": "+", "gene": "trnH", "product": "tRNA-His(gtg)", "amino_acid": "His", "anti_codon": "gtg", "score": 71.9, "nt": "GCGATTGTGGCGAAGTGGTTAACGCACCTGATTGTGGATCAGGCATTCGTGGGTTCAATTCCCATCAGTCGCCCCA", "db_xrefs": [ "SO:0000262" ], "anti_codon_pos": [ 444539, 444541 ], "id": "KMADJIBOEG_15", "locus": "GCA000292445_00810" }, { "type": "cds", "sequence": "contig_1", "start": 444652, "stop": 445173, "strand": "-", "frame": 1, "gene": null, "product": "putative lipoprotein MG348", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47590", "UniRef:UniRef90_P47590" ], "nt": "GTGGATAGAAAAAACGAACTTGCAAGTCAAGAAGATAAAGACAATTCCAATAAGTTAAGAAGATCAGATGCTTCTGCAGTTCGCTATAGAGAAATAGTTGATTTTATTCAACGTACTTATGGTGGTAATGTAGCTAACTTAATCAATCAATCAAATGTGCATGCTCAAACAGTTGGTAATGATGTAACTAAAGCAATTGTGATTGCTTTTAGAAAAGATAACCTTAATAAGATAAGAGCTAATTTTTATGGTTTGGATAACTCTACAAATCCTAATGCACCTGGATCAGGTCAGGGTGATTCAACCCCACCAGCTTCAAGTGGTGAAGGGGGCGGTAGTGATGGCAGTTCAGGTGGTGACTCAAGTAGTGGTAATGGACAAAATACTACTCCTACTTCACCACAATCTTCTCAACCAGCAGTTCAAAGATCACAAAAATCTTATGGAATTAAACAACACGCAGTAAGAGTTAGTGTTGATTTTTTAAATTTCTTGGATAGTGTTTATACTCCACTAAACTAG", "aa": "MDRKNELASQEDKDNSNKLRRSDASAVRYREIVDFIQRTYGGNVANLINQSNVHAQTVGNDVTKAIVIAFRKDNLNKIRANFYGLDNSTNPNAPGSGQGDSTPPASSGEGGGSDGSSGGDSSSGNGQNTTPTSPQSSQPAVQRSQKSYGIKQHAVRVSVDFLNFLDSVYTPLN", "aa_hexdigest": "9738ae84d53004541df9d7f7c059d0ac", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 444652, "stop": 445473, "observations": { "mutation": [ 445200, 445311 ], "stop": [ 445200, 445311 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47590", "query_cov": 1.0, "subject_cov": 0.5372670807453416, "identity": 0.9940000000000001, "score": 295.0, "evalue": 4.14e-98, "gene_start": 1, "gene_end": 173, "reference_start": 150, "reference_end": 322, "reference_sequence": "MKRLFWNLKHKKAWLVLLLGTGMILSSCSNIDQSFFRELSVESVEKNTAYNQLPVNSTTFRNLVFGTRSYNDGNYVVVIATETDSSQINFLNGSTNQGTSSLSWGGTLGTTIRQVQNRYSTYPNGVKFLIWNDIAPGSVKWNPYARFPVVDRKNELASQEDKDNSNKLRRSDASAVRYREIVDFIQRTYGGNVANLINQSNVHAQTVGNDVTKAIVIAFRKDNLNKIRANFYGLDNSTNPNAPGSGQGDSTPPASSGEGGGSDGSSGGDSSSGNGQNTTPTSPQSSQPAVQRSQKSYGIKQHAVRVSVDFLNFLDSVYTPLN" }, "paralog": false, "identity": 0.9926739926739927, "score": 510.0, "evalue": 1.85e-185, "description": "Internal stop codon at 445200, 445311. Nonsense mutation around 445200, 445311.", "psc": { "uniref90_id": "UniRef90_P47590", "product": "Uncharacterized lipoprotein MG348", "uniref50_id": "UniRef50_P47590", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47590", "UniRef:UniRef50_P47590", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47590", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47590" ], "product": "Uncharacterized lipoprotein MG348" } }, "id": "KMADJIBOEG_814", "locus": "GCA000292445_00811" }, { "type": "cds", "sequence": "contig_1", "start": 445309, "stop": 445554, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTAAGTTCTTGTAGTAACATCGATCAGTCTTTTTTTCGTGAACTTTCTGTTGAAAGTGTGGAAAAAAATACAGCTTACAACCAATTGCCTGTTAACAGTACAACATTTAGAAACTTAGTTTTTGGAACCAGATCATATAACGATGGTAATTATGTGGTTGTTATTGCAACCGAAACAGATAGTTCCCAAATTAATTTTCTAAACGGTTCTACTAATCAAGGTACAAGTTCTCTTAGTTGA", "aa": "MILSSCSNIDQSFFRELSVESVEKNTAYNQLPVNSTTFRNLVFGTRSYNDGNYVVVIATETDSSQINFLNGSTNQGTSSLS", "aa_hexdigest": "c86275855398790d5a21928bf08b0e14", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8910.615799999998, "isoelectric_point": 4.440682792663575 }, "id": "KMADJIBOEG_815", "locus": "GCA000292445_00812" }, { "type": "tRNA", "sequence": "contig_1", "start": 445633, "stop": 445720, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(gag)", "amino_acid": "Leu", "anti_codon": "gag", "score": 62.7, "nt": "GTCGGAGTGGTGAAATGGTAGACACGCAAGCTTGAGGTGCTTGTGGTCGTTAAAGACTGTGCCAGTTCAAGTCTGGTCTCCGACACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 445714, 445716 ], "id": "KMADJIBOEG_18", "locus": "GCA000292445_00813" }, { "type": "cds", "sequence": "contig_1", "start": 446073, "stop": 446189, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACCCAACTATTACCGTGTGATCAAAAAAGCAACATCATTTTCTGGATTAGAAATGATCTCTCATGCTTATGGTCAAATTGCGGGAGTGGAAGTTGTTGGGTTTTATTTATGA", "aa": "MQPNYYRVIKKATSFSGLEMISHAYGQIAGVEVVGFYL", "aa_hexdigest": "0915135e70d0a1e36d478ffd60a4f290", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4268.9082, "isoelectric_point": 8.119873619079591 }, "id": "KMADJIBOEG_816", "locus": "GCA000292445_00814" }, { "type": "cds", "sequence": "contig_1", "start": 446343, "stop": 446585, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGAACTTTTTTTTCACCAAAACGCTCTGAAATAACTTTTTGTATTATTGATCCTTTAGATTGGAAAGAATTTAAGCAAAACAAACAATTAAAAGAAAAACTAGTTGAGGCAATGGGAAAAGTTGAATATGACCGAAACTGCTTAGCTTTTGATCAAATCGACAATCTTCAATTTGATAATGCGCTTGAAATCTCTGCTAACTTTGAAGTTAATTTCACTGCAAACCAAAGCGATGTTTGA", "aa": "MRTFFSPKRSEITFCIIDPLDWKEFKQNKQLKEKLVEAMGKVEYDRNCLAFDQIDNLQFDNALEISANFEVNFTANQSDV", "aa_hexdigest": "ce70d05047fff2972ce81ab9881046c1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9427.553499999996, "isoelectric_point": 4.678099250793457 }, "id": "KMADJIBOEG_817", "locus": "GCA000292445_00815" }, { "type": "cds", "sequence": "contig_1", "start": 446703, "stop": 447041, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAACAAATTACTGATTGCATCATCAACAGTACTCAAGAGAATGAGCTTGATTTTCAAAAGTTAGAAATGATGTTTTTTCAAATAGTAAAAAATGATACAGCTCCCATTTTAGAAACAGTTTCAAACAACAAAGACTTTTTTTATAAGAATGAAATTTTAGATGAATCAACAAAAAAAGCAATAACAGACTGTCATGTAAACTTCAATTCTGAAAAATACCTTTTTCTTCTATATGGAAAGATAGATGAATCGCAATTGCAATTAGTTAGACAATTGAGAAGTGATTATCAACTATTAGATAAAGTAATTAATTTAGTATTAGACTTTTCCTTTTGA", "aa": "MQQITDCIINSTQENELDFQKLEMMFFQIVKNDTAPILETVSNNKDFFYKNEILDESTKKAITDCHVNFNSEKYLFLLYGKIDESQLQLVRQLRSDYQLLDKVINLVLDFSF", "aa_hexdigest": "6b6e6d89afb77e2fdca0f6031c37bd01", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13271.977299999988, "isoelectric_point": 4.549472618103027 }, "id": "KMADJIBOEG_818", "locus": "GCA000292445_00816" }, { "type": "cds", "sequence": "contig_1", "start": 447242, "stop": 447457, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTTCACCAGATCAAAAAGATAAAAAGTCATTATTTAAAAAACATTTTGCTTATTTGAAATTAACTGAAAAAGAACTTAATGATCCTAAGATACAAACACTTTTAGAAGTTGCGTGGAATCATTTTGATCAGTGTAGAAAAATTAAAGAAAGAAGATGTAGTAATAAAGGTGATATCCATCTTCAAGCTGTTAGAGAGTGGTTACCTTGA", "aa": "MKISPDQKDKKSLFKKHFAYLKLTEKELNDPKIQTLLEVAWNHFDQCRKIKERRCSNKGDIHLQAVREWLP", "aa_hexdigest": "55370079a5ce544a8a31a663ee684bed", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8563.8949, "isoelectric_point": 9.553714942932128 }, "id": "KMADJIBOEG_819", "locus": "GCA000292445_00817" }, { "type": "cds", "sequence": "contig_1", "start": 447641, "stop": 447901, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTGATTCCAATGATCCAAAATTTACCAGTAACTCCAAGTGATAAAAATAGTTTTGGTTATGCATACAAAAAGATATCTAATCTTTTTGAAACTGAAAAGAAAACAAAGAAAAGGATATTTTTTTTCAATTTACAAAAGAATAACATTAACAATATGGCAGCTTGTATGTTAACTTGTGAAATAGCTAAGAAAAATATAAAAGTTGCTTTAATTTATTGTGAAGAATTTGTTAGCAGATATGACAAAAGTTATTGA", "aa": "MDLIPMIQNLPVTPSDKNSFGYAYKKISNLFETEKKTKKRIFFFNLQKNNINNMAACMLTCEIAKKNIKVALIYCEEFVSRYDKSY", "aa_hexdigest": "f509a4fcb46e1b13e096fd9f505fc189", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10100.777699999995, "isoelectric_point": 9.324723243713382 }, "id": "KMADJIBOEG_820", "locus": "GCA000292445_00818" }, { "type": "tRNA", "sequence": "contig_1", "start": 448235, "stop": 448316, "strand": "+", "gene": "trnL", "product": "tRNA-Leu(caa)", "amino_acid": "Leu", "anti_codon": "caa", "score": 59.5, "nt": "CCTGGAGTGGCGGAATGGTAGACGCGGTGGACTCAAAACCCACTAGGAAACTGTAGGAGTTCAAGTCTCCTCTCCAGGACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 448268, 448270 ], "id": "KMADJIBOEG_16", "locus": "GCA000292445_00819" }, { "type": "cds", "sequence": "contig_1", "start": 448860, "stop": 449069, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGTAATAAACTGGTTTTTTTGCCAAAAAAATCATACCATCAGTTATTTAATATTACTTTTAGTGCAATGATGCTTGCTTTGGCCATCATTACATCATTAATTAGTGAATTTATTAGTATTCCTTTTTTCTCACAACTAAAACTAAATTTTGATATTTCCGTTGTTTTTTTAGTTGCATGTGCTTTTTTTGTGTCACTAGGTTGA", "aa": "MNGNKLVFLPKKSYHQLFNITFSAMMLALAIITSLISEFISIPFFSQLKLNFDISVVFLVACAFFVSLG", "aa_hexdigest": "6e29e4dd51e8106ada634a7a1228fca8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7738.238999999994, "isoelectric_point": 9.035582542419434 }, "id": "KMADJIBOEG_821", "locus": "GCA000292445_00820" }, { "type": "cds", "sequence": "contig_1", "start": 449117, "stop": 449278, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00719.24" ], "nt": "ATGGACAAATTTTTAATTGATGTTATTGTAGAAATCCCTAAACACAGCAAAATAAAGTATGAGTATGATCGTCAAACTGGTCAAATTCGCGTTGATAGAATCCTATTTGGAAGTGAATCATATCCACAAAACTACGGTTTTATTAAAAATACATTAGATTGA", "aa": "MDKFLIDVIVEIPKHSKIKYEYDRQTGQIRVDRILFGSESYPQNYGFIKNTLD", "aa_hexdigest": "0ce5775ebd71521e2fdb7f70f3363db7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00719.24", "name": "Pyrophosphatase", "length": 45, "aa_cov": 0.8490566037735849, "hmm_cov": 1.0, "evalue": 9.221762499327324e-18, "score": 59.657264709472656, "start": 8, "stop": 52 } ], "seq_stats": { "molecular_weight": 6323.1482, "isoelectric_point": 6.546112632751465 }, "id": "KMADJIBOEG_822", "locus": "GCA000292445_00821" }, { "type": "cds", "sequence": "contig_1", "start": 449339, "stop": 449557, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00719.24" ], "nt": "GTGCCTACAAGAATTGTAGGAGCACTTGAGATGATTGATGATGGGGAAATTGATACTAAGTTATTAGGAGTTATTGATTGTGACCCTAGATATAAAGAAATTAATCAAATTAGTGATTTACCTAAACATAGAATAGAAGAAATTCTTATCTTTTTAAAAACTTATAAATTACTTCAAAAAAAGACTGTAATTATTAAGGGTTTAAAAGATGTTTGTTGA", "aa": "MPTRIVGALEMIDDGEIDTKLLGVIDCDPRYKEINQISDLPKHRIEEILIFLKTYKLLQKKTVIIKGLKDVC", "aa_hexdigest": "acb30d4e67b259dd1258e2fa1f954240", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00719.24", "name": "Pyrophosphatase", "length": 69, "aa_cov": 0.9583333333333334, "hmm_cov": 1.0, "evalue": 3.994639479865239e-19, "score": 64.08428192138672, "start": 2, "stop": 70 } ], "seq_stats": { "molecular_weight": 8282.800799999994, "isoelectric_point": 6.551682853698731 }, "id": "KMADJIBOEG_823", "locus": "GCA000292445_00822" }, { "type": "cds", "sequence": "contig_1", "start": 449866, "stop": 450174, "strand": "+", "frame": 1, "gene": "recU", "product": "Holliday junction resolvase RecU", "db_xrefs": [ "COG:COG3331", "COG:R", "EC:3.1.21.10", "GO:0000287", "GO:0003676", "GO:0004519", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0007059", "KEGG:K03700", "SO:0001217", "UniRef:UniRef50_Q49422", "UniRef:UniRef90_Q49422" ], "nt": "ATGTATTTTGATTTTGAAGCAAAACAAACAAATAAAAGTAATTTTCCAATTGCTCAAATAGCAGAACACCAATTAAATCATTTGAAAAGAATTGACCAAATTGGAGGAGTTAGCTTCTTGCTAATTTACTTTCAAACTAAAGATCAAATTTTTGCATTTCACACTAAGGATCTTCTAGAAACAATAAAAAATCAAGAAAGCAAAACAATTAAAAGGGAATTGATAGAACAAAAATCTCAGAAAGTTCCACTATTGTATCCTGGTATTATTGATTTAATTTCAATAATTCAAAGCTTTAAAAATTATTAG", "aa": "MYFDFEAKQTNKSNFPIAQIAEHQLNHLKRIDQIGGVSFLLIYFQTKDQIFAFHTKDLLETIKNQESKTIKRELIEQKSQKVPLLYPGIIDLISIIQSFKNY", "aa_hexdigest": "765350889a981d7f6d3b937009619494", "start_type": "ATG", "rbs_motif": null, "genes": [ "recU" ], "truncated": "5-prime", "pseudogene": { "start": 449674, "stop": 450174, "observations": { "mutation": [ 449755 ], "stop": [ 449755 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49422", "query_cov": 1.0, "subject_cov": 0.6144578313253012, "identity": 1.0, "score": 202.0, "evalue": 3.09e-65, "gene_start": 1, "gene_end": 102, "reference_start": 65, "reference_end": 166, "reference_sequence": "MINANRGMLLETIVNQTIARLKDHPDIWLEKRFLPIKPIAFRHAHVSGNVSQKSKTDYYGIYKGMYFDFEAKQTNKSNFPIAQIAEHQLNHLKRIDQIGGVSFLLIYFQTKDQIFAFHTKDLLETIKNQESKTIKRELIEQKSQKVPLLYPGIIDLISIIQSFKNY" }, "paralog": false, "identity": 0.9939759036144579, "score": 328.0, "evalue": 3.06e-117, "description": "Internal stop codon at 449755. Nonsense mutation around 449755.", "psc": { "uniref90_id": "UniRef90_Q49422", "gene": "recU", "product": "Holliday junction resolvase RecU", "ec_ids": [ "3.1.21.10" ], "uniref50_id": "UniRef50_Q49422", "cog_id": "COG3331", "cog_category": "R", "kegg_orthology_id": "K03700", "go_ids": [ "GO:0000287", "GO:0003676", "GO:0004519", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0007059" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49422", "EC:3.1.21.10", "UniRef:UniRef50_Q49422", "COG:COG3331", "COG:R", "KEGG:K03700", "GO:0000287", "GO:0003676", "GO:0004519", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0007059" ] }, "pscc": { "uniref50_id": "UniRef50_Q49422", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49422" ], "product": "Holliday junction resolvase RecU" } }, "id": "KMADJIBOEG_824", "locus": "GCA000292445_00823" }, { "type": "cds", "sequence": "contig_1", "start": 450186, "stop": 450500, "strand": "-", "frame": 2, "gene": "hupA", "product": "putative MG353-like protein", "db_xrefs": [ "COG:B", "COG:COG0776", "GO:0003677", "GO:0030527", "SO:0001217", "UniRef:UniRef50_P47595", "UniRef:UniRef90_P47595" ], "nt": "ATGGAAAAAACATCAAATACAAGTAAGCCACTTTCTCGTAGTGAAATCAATAAAATAATTGCAGTTGCTACTGGTATAAAAGAAAAAAAAATTAAGGAAATCTTTAAATACCTTAACACATTGTTACTAAATGAATTGGTAAGTAGAAGTGTTTGTATATTACCTGAAAATTTAGGTAAATTAAGGATTACTATTAGAAATGCACGTTATCAGAAGGATATGCAAACGGGTGAGATTAGACATATCCCACCAAAACCATTAGTGCGCTACAGTCCAAGCAAAACGATCAAAGAAACTGCAGCTAAAGTGCGTTGA", "aa": "MEKTSNTSKPLSRSEINKIIAVATGIKEKKIKEIFKYLNTLLLNELVSRSVCILPENLGKLRITIRNARYQKDMQTGEIRHIPPKPLVRYSPSKTIKETAAKVR", "aa_hexdigest": "2f0e07af14c4a3b6afd04bd9a12ba70a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47595", "query_cov": 1.0, "subject_cov": 0.9541284403669725, "identity": 1.0, "score": 193.0, "evalue": 2.41e-62, "valid": true, "gene": "HupA", "product": "Uncharacterized protein MG353 homolog", "uniref50_id": "UniRef50_P47595", "cog_id": "COG0776", "cog_category": "B", "go_ids": [ "GO:0003677", "GO:0030527" ] }, "pscc": { "uniref50_id": "UniRef50_P47595", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47595" ], "product": "Uncharacterized protein MG353" }, "genes": [ "hupA" ], "id": "KMADJIBOEG_825", "locus": "GCA000292445_00824" }, { "type": "cds", "sequence": "contig_1", "start": 450649, "stop": 451062, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MG354", "db_xrefs": [ "RefSeq:WP_009885812.1", "SO:0001217", "UniParc:UPI000013968F", "UniRef:UniRef100_P47596", "UniRef:UniRef50_P47596", "UniRef:UniRef90_P47596" ], "nt": "ATGGAACAAAACAATATTAAAGAACAACTTATTTCTTTCTTTAATCAAGCATGTTCTACCCACCAAGAAAGACTTGATTTTATCTGTTCTACAAGAGAAAGTGATACTTTTTCTAGTGTTGATGTACCACTTGAACCCATTAAAAATATTATTGAAATAACTAAAGATGAAAATCAACAAATTGAAATTACAAAAATAGCTGTTAATAACATTAAAACATTATCTTCTGTTGGTGCAACCGGTCAGTATATGGCATCTTTCTTTTCAACAAATAGTGAGCCAGCTATAATATTTTGCGTCATTTATTTTTTATATCACTTTGGTTTTTTAAAAGATAACAATAAAAAACAAATAATAAAAAAGGCTTATGAAACTATTGCTGATAACATTGCTGACTATTTAAATGAAAATTAG", "aa": "MEQNNIKEQLISFFNQACSTHQERLDFICSTRESDTFSSVDVPLEPIKNIIEITKDENQQIEITKIAVNNIKTLSSVGATGQYMASFFSTNSEPAIIFCVIYFLYHFGFLKDNNKKQIIKKAYETIADNIADYLNEN", "aa_hexdigest": "7aae0a269f2241d48b8b839d81d6664a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013968F", "ncbi_nrp_id": "WP_009885812.1", "uniref100_id": "UniRef100_P47596", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013968F", "RefSeq:WP_009885812.1", "UniRef:UniRef100_P47596" ] }, "ips": { "uniref100_id": "UniRef100_P47596", "uniref90_id": "UniRef90_P47596" }, "psc": { "uniref90_id": "UniRef90_P47596", "product": "Uncharacterized protein MG354", "uniref50_id": "UniRef50_P47596" }, "pscc": { "uniref50_id": "UniRef50_P47596", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47596" ], "product": "Uncharacterized protein MG354" }, "genes": [], "id": "KMADJIBOEG_826", "locus": "GCA000292445_00825" }, { "type": "cds", "sequence": "contig_1", "start": 451065, "stop": 451949, "strand": "-", "frame": 2, "gene": "clpB", "product": "Chaperone protein ClpB", "db_xrefs": [ "COG:COG0542", "COG:O", "GO:0005524", "GO:0005737", "GO:0016887", "GO:0034605", "SO:0001217", "UniRef:UniRef50_P47597", "UniRef:UniRef90_P47597" ], "nt": "TTGCACTTAGGTGATGAAATCAAAAAAAGAGTTAAAGGACAAGATGAAGCCATCGATGCTGTTGTTAACACTGTAATTAGAGGTAGAGTAAATATAAATGACCCAAACAAACCTATTGGTTCTTTCATCTTTTTAGGTTCTACTGGTGTTGGTAAAACTGAACTTGCCAAATCATTAGCAGAAGTTCTTTTTGACAATGAAAAAGCTCTGATTCGTTTTGATATGAGTGAATATATGGAAAAACATTCAGTAGCTAAATTAATTGGTGCACCCCCAGGGTACATAGGTTATGAACAATCAGGTTTGCTAACTGAAGCGGTTAGAAGAAAACCTTATAGCGTCTTGTTATTTGATGAAATTGAAAAGGCACATCCTGATGTAACTAATGTTTTATTACAAGTTTTAGATGATGGTACTTTAAAAGATTCACAAGGAAGGGTTGTTAATTTCAAAAATACTTTGATAATTATGACTTCTAACCTAGGTTCAAATTTTCTTTTAGAAGGAAAAAAAGATTTGGCCATTCAAAGTCTAAAGAAACATTTCCGTCCTGAATTTATAAATCGTATTGATGAGATAGTATTTTTCAATGTTCTTGAGAAAGATACAGTTTTATCGATAATCAACAGCTTGTTGGCACAACTTTCAAAACGCTTGAATAAACAAAATTTATTTTTTAATTTTGATTCAAATCTAACAGAGTTTATCTATAAAAGTAGTTTTGATCAACAGTTTGGTGCAAGACCAATTAAGCGCTTTATTGATCATAGTGTTGCAACTTTAATAGCTAAATATATCCTTCAGGGAAAGATAAAAAAAGGTGTTGGATACAACATTGCAGTTGTTAAAGACAATATTACCATTACACAAAATAATAAGTCTTAA", "aa": "MHLGDEIKKRVKGQDEAIDAVVNTVIRGRVNINDPNKPIGSFIFLGSTGVGKTELAKSLAEVLFDNEKALIRFDMSEYMEKHSVAKLIGAPPGYIGYEQSGLLTEAVRRKPYSVLLFDEIEKAHPDVTNVLLQVLDDGTLKDSQGRVVNFKNTLIIMTSNLGSNFLLEGKKDLAIQSLKKHFRPEFINRIDEIVFFNVLEKDTVLSIINSLLAQLSKRLNKQNLFFNFDSNLTEFIYKSSFDQQFGARPIKRFIDHSVATLIAKYILQGKIKKGVGYNIAVVKDNITITQNNKS", "aa_hexdigest": "2769379323d5850f6787d64dc1c2fdfb", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00265DA75C", "query_cov": 0.9659863945578231, "subject_cov": 0.8279883381924198, "identity": 0.5770000000000001, "score": 323.0, "evalue": 3.73e-107, "valid": false, "product": "AAA family ATPase", "uniref50_id": "UniRef50_A0A661I2M2" }, "genes": [ "clpB" ], "truncated": "3-prime", "pseudogene": { "start": 451065, "stop": 452249, "observations": { "mutation": [ 452246 ], "stop": [ 452246 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47597", "query_cov": 1.0, "subject_cov": 0.4117647058823529, "identity": 0.997, "score": 569.0, "evalue": 1.96e-198, "gene_start": 1, "gene_end": 294, "reference_start": 421, "reference_end": 714, "reference_sequence": "MNINFTPAGENRNFLQEIGRNINDEVLKNKVDPIIGRDNEIRRLIEILSRKSKNNPVLIGEPGVGKTAIVEGFVRRVVSNDVPLNLRDVEIYELSLSGLIAGTKFQGEFEKRINTILKQVKESNGRIILFIDEIHQIVGLGRNSSSGAMDIANILKPMLARGEIKVIGATTLKEYREYIEKDGALERRFQKILINEPSSQEALTIMRGLKTRWELFHNITIFDSALVAAVEMSTRYINERNLPDKAIDLIDEAAAKIKTEMSSEPVAIDSLKREIINLETEYAALKQDKENDNKQSKKEYLEKLKKQLDALKQKRDSLINEWKKEKADFENINKLKKEIEEFQTKLETYQSEGNYESASKILYSDIPRLKKELESAQQKYATSKHDLFKTEVSENEIAEVISQTTGIPLKKLLESEKDKLLHLGDEIKKRVKGQDEAIDAVVNTVIRGRVNINDPNKPIGSFIFLGSTGVGKTELAKSLAEVLFDNEKALIRFDMSEYMEKHSVAKLIGAPPGYIGYEQSGLLTEAVRRKPYSVLLFDEIEKAHPDVTNVLLQVLDDGTLKDSQGRVVNFKNTLIIMTSNLGSNFLLEGKKDLAIQSLKKHFRPEFINRIDEIVFFNVLEKDTVLSIINSLLAQLSKRLNKQNLFFNFDSNLTEFIYKSSFDQQFGARPIKRFIDHSVATLIAKYILQGKIKKGVGYNIAVVKDNITITQNNKS" }, "paralog": false, "identity": 0.9974619289340102, "score": 738.0, "evalue": 1.73e-267, "description": "Internal stop codon at 452246. Nonsense mutation around 452246.", "psc": { "uniref90_id": "UniRef90_P47597", "gene": "clpB", "product": "Chaperone protein ClpB", "uniref50_id": "UniRef50_P47597", "cog_id": "COG0542", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0016887", "GO:0034605" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47597", "UniRef:UniRef50_P47597", "COG:COG0542", "COG:O", "GO:0005737", "GO:0005524", "GO:0016887", "GO:0034605" ] }, "pscc": { "uniref50_id": "UniRef50_P47597", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47597" ], "product": "Chaperone protein ClpB" } }, "id": "KMADJIBOEG_827", "locus": "GCA000292445_00826" }, { "type": "cds", "sequence": "contig_1", "start": 452285, "stop": 452515, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17871.7" ], "nt": "ATGTCAACTCGTTATATTAATGAACGTAATTTACCTGATAAAGCCATTGATCTTATTGATGAGGCTGCTGCTAAGATCAAAACAGAAATGTCATCTGAACCAGTTGCAATAGATAGTCTTAAACGTGAAATAATCAATCTTGAGACAGAGTATGCAGCTCTTAAACAAGATAAGGAAAATGATAACAAACAATCAAAGAAAGAATATTTAGAGAAACTAAAAAACAATTAG", "aa": "MSTRYINERNLPDKAIDLIDEAAAKIKTEMSSEPVAIDSLKREIINLETEYAALKQDKENDNKQSKKEYLEKLKNN", "aa_hexdigest": "6bdbf5ff6ecb02222dcc04611d4aafba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17871.7", "name": "AAA_lid_9", "length": 67, "aa_cov": 0.881578947368421, "hmm_cov": 1.0, "evalue": 1.5103302932513026e-20, "score": 68.23301696777344, "start": 1, "stop": 67 } ], "seq_stats": { "molecular_weight": 8798.831599999994, "isoelectric_point": 5.177941322326659 }, "id": "KMADJIBOEG_828", "locus": "GCA000292445_00827" }, { "type": "cds", "sequence": "contig_1", "start": 452570, "stop": 453208, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00004.35", "PFAM:PF13191.12", "PFAM:PF01637.24", "PFAM:PF05621.17" ], "nt": "ATGAATATTAATTTCACACCTGCTGGTGAAAATCGTAATTTTTTGCAAGAAATTGGTCGTAATATTAACGATGAAGTATTAAAAAATAAGGTCGATCCTATTATTGGAAGAGATAACGAAATTCGTCGTTTAATTGAGATATTAAGTCGTAAAAGCAAAAACAATCCTGTTTTAATTGGTGAACCTGGAGTTGGTAAAACCGCAATAGTAGAAGGTTTTGTTAGAAGAGTTGTTAGTAATGATGTACCTTTAAATTTAAGGGATGTAGAAATTTATGAACTATCTCTTTCTGGATTAATTGCTGGCACTAAATTCCAAGGTGAATTTGAAAAAAGAATTAATACCATTCTTAAGCAAGTAAAAGAATCAAATGGCAAGATTATTCTTTTTATTGATGAAATTCACCAAATAGTTGGATTAGGACGTAATTCTAGCAGTGGTGCAATGGATATTGCCAATATATTAAAGCCGATGCTAGCTCGAGGAGAAATAAAAGTAATTGGCGCTACTACTCTAAAAGAATACCGGGAATACATTGAAAAAGATGGCGCTTTAGAACGTAGATTTCAAAAAATTCTTATTAACGAGCCTAGTAGTCAAGAGGCACTAACAATTATGCGTGGTTTAAAAACACGTTGA", "aa": "MNINFTPAGENRNFLQEIGRNINDEVLKNKVDPIIGRDNEIRRLIEILSRKSKNNPVLIGEPGVGKTAIVEGFVRRVVSNDVPLNLRDVEIYELSLSGLIAGTKFQGEFEKRINTILKQVKESNGKIILFIDEIHQIVGLGRNSSSGAMDIANILKPMLARGEIKVIGATTLKEYREYIEKDGALERRFQKILINEPSSQEALTIMRGLKTR", "aa_hexdigest": "88874d5ce2180fc5c60e7c012e61086d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001C4E8114", "query_cov": 0.8584905660377359, "subject_cov": 0.9479166666666666, "identity": 0.621, "score": 238.0, "evalue": 5.63e-77, "valid": false, "product": "AAA family ATPase", "uniref50_id": "UniRef50_J9G8B9" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00004.35", "name": "AAA", "length": 142, "aa_cov": 0.6367924528301887, "hmm_cov": 0.8732394366197183, "evalue": 2.894846897454019e-18, "score": 61.74818801879883, "start": 57, "stop": 191 }, { "id": "PF13191.12", "name": "AAA_16", "length": 51, "aa_cov": 0.22641509433962265, "hmm_cov": 1.0, "evalue": 1.9094703489398977e-10, "score": 36.623321533203125, "start": 34, "stop": 81 }, { "id": "PF01637.24", "name": "ATPase_2", "length": 190, "aa_cov": 0.8066037735849056, "hmm_cov": 0.9947368421052631, "evalue": 5.651492453924866e-11, "score": 37.77372360229492, "start": 37, "stop": 207 }, { "id": "PF05621.17", "name": "TniB", "length": 168, "aa_cov": 0.7311320754716981, "hmm_cov": 0.9702380952380952, "evalue": 1.1838890608334423e-10, "score": 36.29184341430664, "start": 40, "stop": 194 } ], "seq_stats": { "molecular_weight": 23837.309700000016, "isoelectric_point": 9.42039432525635 }, "id": "KMADJIBOEG_829", "locus": "GCA000292445_00828" }, { "type": "cds", "sequence": "contig_1", "start": 453845, "stop": 454204, "strand": "-", "frame": 3, "gene": "cotS", "product": "putative protein MG356", "db_xrefs": [ "COG:COG0510", "COG:H", "GO:0004305", "GO:0005737", "GO:0006646", "SO:0001217", "UniRef:UniRef50_Q49423", "UniRef:UniRef90_Q49423" ], "nt": "ATGGATTTAAAAAAAGCGGTTGCTTTTCTTTGTGAAAAACTTCAAGTTGTAGAAACAGATATTTTCAAGATAGAGCAAATTCATTCTGGTTTTACTAATTTTTCTTTTTTTTGTGAATTGAAAGATCATAGTAAATTTCAAATAAGACTTTCAAGAAAAGATGTTATTTTAGACAGAAGAAATGAACAAATTATTTTGCAACTAGTAGAACCTTTTTTTTCAAACCCATTTGTTTATTTTGATGTAACAACAGGTAATGCTATAAAAAGGTGGATAGAAGGCACTCAACCTAAAAGAGCAACTGATCTATTTTTATGCAGATTGGTTGAATCTTTAAAAAAAGTTCATTCAGTTGACTGA", "aa": "MDLKKAVAFLCEKLQVVETDIFKIEQIHSGFTNFSFFCELKDHSKFQIRLSRKDVILDRRNEQIILQLVEPFFSNPFVYFDVTTGNAIKRWIEGTQPKRATDLFLCRLVESLKKVHSVD", "aa_hexdigest": "94cdca7201e50f6974daa0173640bf07", "start_type": "ATG", "rbs_motif": null, "genes": [ "cotS" ], "truncated": "5-prime", "pseudogene": { "start": 453542, "stop": 454204, "observations": { "mutation": [ 453643, 453682, 453847 ], "stop": [ 453643, 453682, 453847 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49423", "query_cov": 1.0, "subject_cov": 0.425, "identity": 1.0, "score": 242.0, "evalue": 7.53e-79, "gene_start": 1, "gene_end": 119, "reference_start": 1, "reference_end": 119, "reference_sequence": "MDLKKAVAFLCEKLQVVETDIFKIEQIHSGFTNFSFFCELKDHSKFQIRLSRKDVILDRRNEQIILQLVEPFFSNPFVYFDVTTGNAIKRWIEGTQPKRATDLFLCRLVESLKKVHSVDWKPFANELLIFDPLVYFEKTKLSAFYKRLYLNLTKKHIDIPKTLCHHDATFDNLVWTPKKQVILIDFEWSCVDNPYYEIANIIREELTIETANKLIDIYGGLKRKLVFETVIYVLLFAFQWTEIMPQTEAILNYRKWVEKRLEYFLKALFPSIWKQAQKNS" }, "paralog": false, "identity": 0.9863636363636363, "score": 435.0, "evalue": 1.68e-157, "description": "Internal stop codon at 453643, 453682, 453847. Nonsense mutation around 453643, 453682, 453847.", "psc": { "uniref90_id": "UniRef90_Q49423", "gene": "CotS", "product": "Uncharacterized protein MG356", "uniref50_id": "UniRef50_Q49423", "cog_id": "COG0510", "cog_category": "H", "go_ids": [ "GO:0005737", "GO:0004305", "GO:0006646" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49423", "UniRef:UniRef50_Q49423", "COG:COG0510", "COG:H", "GO:0005737", "GO:0004305", "GO:0006646" ] }, "pscc": { "uniref50_id": "UniRef50_Q49423", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49423" ], "product": "Uncharacterized protein MG356" } }, "id": "KMADJIBOEG_830", "locus": "GCA000292445_00829" }, { "type": "cds", "sequence": "contig_1", "start": 454217, "stop": 454729, "strand": "-", "frame": 3, "gene": "ackA", "product": "Acetate kinase", "db_xrefs": [ "COG:C", "COG:COG0282", "EC:2.7.2.1", "GO:0000287", "GO:0005524", "GO:0005737", "GO:0006083", "GO:0006085", "GO:0008776", "KEGG:K00925", "SO:0001217", "UniRef:UniRef50_P75245", "UniRef:UniRef90_P47599" ], "nt": "ATGGGATTCACTCCCCTTGAAGGATTAATAATGGGAACACGTAGTGGTGATATTGATCCTGCCATTGTTAGTTACATTGCTGAACAGCAAAAGCTTTCATGTAATGATGTTGTAAATGAATTAAATAAAAAGAGTGGAATGTTTGCTATAACAGGTAGTTCTGACATGCGTGATATTTTTGATAAACCAGAAATTAATGATATTGCTATAAAAATGTATGTTAATCGTGTTGCTGACTATATTGCTAAATACCTAAATCAACTTTCAGGTGAAATTGATAGCTTGGTATTTACTGGGGGAGTTGGTGAAAATGCTAGTTATTGTGTGCAATTAATAATTGAAAAAGTTGCTTCACTTGGTTTTAAAACTAACAGTAATTTATTTGGAAATTATCAAGATAGTTCTCTAATTTCAACAAATGAAAGCAAGTATCAAATTTTTAGAGTTCGTACAAATGAGGAATTGATGATTGTAGAAGATGCTTTGAGAGTAAGTACAAACATTAAAAAATAA", "aa": "MGFTPLEGLIMGTRSGDIDPAIVSYIAEQQKLSCNDVVNELNKKSGMFAITGSSDMRDIFDKPEINDIAIKMYVNRVADYIAKYLNQLSGEIDSLVFTGGVGENASYCVQLIIEKVASLGFKTNSNLFGNYQDSSLISTNESKYQIFRVRTNEELMIVEDALRVSTNIKK", "aa_hexdigest": "7ec6ac10e44b468ab21afd7c5c0886d4", "start_type": "ATG", "rbs_motif": null, "genes": [ "ackA" ], "truncated": "3-prime", "pseudogene": { "start": 454217, "stop": 455028, "observations": { "deletion": [ 454827 ], "mutation": [ 454902 ], "stop": [ 454902 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47599", "query_cov": 1.0, "subject_cov": 0.43256997455470736, "identity": 1.0, "score": 332.0, "evalue": 6.89e-112, "gene_start": 1, "gene_end": 170, "reference_start": 224, "reference_end": 393, "reference_sequence": "MQSHKILVVNAGSSSIKFQLFNDKKQVLAKGLCERIFIDGFFKLEFNQKKIEEKVQFNDHNLAVKHFLNALKKNKIITELSEIGLIGHRVVQGANYFTDAVLVDTHSLAKIKEFIKLAPLHNKPEADVIEIFLKEIKTAKNVAVFDTTFHTTIPRENYLYAVPENWEKNNLVRRYGFHGTSYKYINEFLEKKFNKKPLNLIVCHLGNGASVCAIKQGKSLNTSMGFTPLEGLIMGTRSGDIDPAIVSYIAEQQKLSCNDVVNELNKKSGMFAITGSSDMRDIFDKPEINDIAIKMYVNRVADYIAKYLNQLSGEIDSLVFTGGVGENASYCVQLIIEKVASLGFKTNSNLFGNYQDSSLISTNESKYQIFRVRTNEELMIVEDALRVSTNIKK" }, "paralog": false, "identity": 0.9962962962962963, "score": 526.0, "evalue": 6.15e-191, "description": "Internal stop codon at 454902. Frameshift due to deletion around 454827. Nonsense mutation around 454902.", "psc": { "uniref90_id": "UniRef90_P47599", "gene": "ackA", "product": "Acetate kinase", "ec_ids": [ "2.7.2.1" ], "uniref50_id": "UniRef50_P75245", "cog_id": "COG0282", "cog_category": "C", "kegg_orthology_id": "K00925", "go_ids": [ "GO:0005737", "GO:0008776", "GO:0005524", "GO:0000287", "GO:0006083", "GO:0006085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47599", "EC:2.7.2.1", "UniRef:UniRef50_P75245", "COG:COG0282", "COG:C", "KEGG:K00925", "GO:0005737", "GO:0008776", "GO:0005524", "GO:0000287", "GO:0006083", "GO:0006085" ] }, "pscc": { "uniref50_id": "UniRef50_P75245", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75245" ], "product": "Acetate kinase" } }, "id": "KMADJIBOEG_831", "locus": "GCA000292445_00830" }, { "type": "cds", "sequence": "contig_1", "start": 454900, "stop": 455397, "strand": "-", "frame": 1, "gene": "ackA", "product": "Acetate kinase", "db_xrefs": [ "COG:C", "COG:COG0282", "EC:2.7.2.1", "GO:0000287", "GO:0005524", "GO:0005737", "GO:0006083", "GO:0006085", "GO:0008776", "KEGG:K00925", "SO:0001217", "UniRef:UniRef50_P75245", "UniRef:UniRef90_P47599" ], "nt": "ATGCAAAGTCACAAAATCTTGGTTGTTAATGCAGGTAGCAGTTCAATTAAATTTCAACTTTTTAATGATAAAAAACAAGTACTAGCTAAAGGACTTTGTGAACGTATTTTCATTGATGGTTTTTTTAAGCTTGAATTTAATCAAAAAAAGATAGAAGAAAAGGTTCAATTTAATGATCATAATCTTGCTGTTAAGCATTTTTTAAATGCGCTTAAAAAAAACAAAATTATTACTGAACTTTCAGAAATTGGGCTAATAGGGCATAGAGTAGTACAAGGAGCAAATTATTTTACAGATGCAGTTCTTGTTGATACACATTCACTAGCAAAAATAAAAGAATTCATTAAGTTAGCACCGCTTCATAATAAACCAGAAGCAGATGTTATTGAAATTTTTCTAAAAGAGATAAAAACTGCTAAGAATGTTGCTGTATTTGATACCACTTTTCACACTACTATTCCAAGGGAAAATTATCTTTATGCAGTTCCTGAAAATTGA", "aa": "MQSHKILVVNAGSSSIKFQLFNDKKQVLAKGLCERIFIDGFFKLEFNQKKIEEKVQFNDHNLAVKHFLNALKKNKIITELSEIGLIGHRVVQGANYFTDAVLVDTHSLAKIKEFIKLAPLHNKPEADVIEIFLKEIKTAKNVAVFDTTFHTTIPRENYLYAVPEN", "aa_hexdigest": "74bbfad2789e566929303c95e3086fd4", "start_type": "ATG", "rbs_motif": null, "genes": [ "ackA" ], "truncated": "5-prime", "pseudogene": { "start": 454598, "stop": 455397, "observations": { "deletion": [ 454827 ], "mutation": [ 454902 ], "stop": [ 454902 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47599", "query_cov": 1.0, "subject_cov": 0.4198473282442748, "identity": 1.0, "score": 328.0, "evalue": 2.62e-110, "gene_start": 1, "gene_end": 165, "reference_start": 1, "reference_end": 165, "reference_sequence": "MQSHKILVVNAGSSSIKFQLFNDKKQVLAKGLCERIFIDGFFKLEFNQKKIEEKVQFNDHNLAVKHFLNALKKNKIITELSEIGLIGHRVVQGANYFTDAVLVDTHSLAKIKEFIKLAPLHNKPEADVIEIFLKEIKTAKNVAVFDTTFHTTIPRENYLYAVPENWEKNNLVRRYGFHGTSYKYINEFLEKKFNKKPLNLIVCHLGNGASVCAIKQGKSLNTSMGFTPLEGLIMGTRSGDIDPAIVSYIAEQQKLSCNDVVNELNKKSGMFAITGSSDMRDIFDKPEINDIAIKMYVNRVADYIAKYLNQLSGEIDSLVFTGGVGENASYCVQLIIEKVASLGFKTNSNLFGNYQDSSLISTNESKYQIFRVRTNEELMIVEDALRVSTNIKK" }, "paralog": false, "identity": 0.9962406015037594, "score": 522.0, "evalue": 2.38e-189, "description": "Internal stop codon at 454902. Frameshift due to deletion around 454827. Nonsense mutation around 454902.", "psc": { "uniref90_id": "UniRef90_P47599", "gene": "ackA", "product": "Acetate kinase", "ec_ids": [ "2.7.2.1" ], "uniref50_id": "UniRef50_P75245", "cog_id": "COG0282", "cog_category": "C", "kegg_orthology_id": "K00925", "go_ids": [ "GO:0005737", "GO:0008776", "GO:0005524", "GO:0000287", "GO:0006083", "GO:0006085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47599", "EC:2.7.2.1", "UniRef:UniRef50_P75245", "COG:COG0282", "COG:C", "KEGG:K00925", "GO:0005737", "GO:0008776", "GO:0005524", "GO:0000287", "GO:0006083", "GO:0006085" ] }, "pscc": { "uniref50_id": "UniRef50_P75245", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75245" ], "product": "Acetate kinase" } }, "id": "KMADJIBOEG_832", "locus": "GCA000292445_00831" }, { "type": "cds", "sequence": "contig_1", "start": 455505, "stop": 455714, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_014894048.1", "SO:0001217", "UniParc:UPI00027B3165", "UniRef:UniRef100_UPI00027B3165", "UniRef:UniRef50_A0AAN4XFQ7", "UniRef:UniRef90_UPI00027B3165" ], "nt": "ATGAAAATTAAAAAATGCTATATTATCGCTGTTATTTCAGCTTTTATTGGTTTTCTTTTTTACTTTTTATCTTCACTTATAGGGATAACAGATGTTAATAATGCAAAAACAGTAGCAACTGTATTTTTTGTATTAGCAACATTAATAGTTGTTAATGCTATTGTCTTCTGGATTCTTTATCTATTCAAAAAAAAGAACGTAGTTAAGTAA", "aa": "MKIKKCYIIAVISAFIGFLFYFLSSLIGITDVNNAKTVATVFFVLATLIVVNAIVFWILYLFKKKNVVK", "aa_hexdigest": "2aec87b6506e412f4fc76993107521a6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3165", "ncbi_nrp_id": "WP_014894048.1", "uniref100_id": "UniRef100_UPI00027B3165", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3165", "RefSeq:WP_014894048.1", "UniRef:UniRef100_UPI00027B3165" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3165", "uniref90_id": "UniRef90_UPI00027B3165" }, "psc": { "uniref90_id": "UniRef90_UPI00027B3165", "uniref50_id": "UniRef50_A0AAN4XFQ7" }, "pscc": { "uniref50_id": "UniRef50_A0AAN4XFQ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN4XFQ7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7823.556999999997, "isoelectric_point": 9.937430763244631 }, "id": "KMADJIBOEG_833", "locus": "GCA000292445_00832" }, { "type": "cds", "sequence": "contig_1", "start": 455777, "stop": 456004, "strand": "+", "frame": 2, "gene": null, "product": "MPN534 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75244", "UniRef:UniRef90_UPI0001B417BC" ], "nt": "ATGGAAACTATCAGAGAAACACTTTCTTCTATACAAAGAAGCAATTTTTTATTAAATGAAAGCGCACAAATTCTCAATACGTGTGTAAACAAGAACACATTGCTTGACGATAAGCTGCGCAATTCGATTAATTTTGCACTTAGCATCATTAAGCTAGCAGACTTTTGTCTAAAGGATGATACTGAAAAAATTAATCAATTCTTAGGTAAAACATATGATTTACCATGA", "aa": "METIRETLSSIQRSNFLLNESAQILNTCVNKNTLLDDKLRNSINFALSIIKLADFCLKDDTEKINQFLGKTYDLP", "aa_hexdigest": "db9c1096daeeb392e83726590a16739b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 455777, "stop": 456202, "observations": { "mutation": [ 456002 ], "stop": [ 456002 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001B417BC", "query_cov": 1.0, "subject_cov": 0.5319148936170213, "identity": 1.0, "score": 148.0, "evalue": 1.92e-44, "gene_start": 1, "gene_end": 75, "reference_start": 1, "reference_end": 75, "reference_sequence": "METIRETLSSIQRSNFLLNESAQILNTCVNKNTLLDDKLRNSINFALSIIKLADFCLKDDTEKINQFLGKTYDLPWEFQFNQKDKHIECTVKDNFGREKLINLIFQIGDAIETYHTTLIRFKIPKHCLNARDQIKKIMEGK" }, "paralog": false, "identity": 0.9929078014184397, "score": 281.0, "evalue": 1.71e-99, "description": "Internal stop codon at 456002. Nonsense mutation around 456002.", "psc": { "uniref90_id": "UniRef90_UPI0001B417BC", "product": "MPN534 family protein", "uniref50_id": "UniRef50_P75244", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001B417BC", "UniRef:UniRef50_P75244" ] }, "pscc": { "uniref50_id": "UniRef50_P75244", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75244" ], "product": "Uncharacterized protein MPN_534" } }, "id": "KMADJIBOEG_834", "locus": "GCA000292445_00833" }, { "type": "cds", "sequence": "contig_1", "start": 456322, "stop": 456822, "strand": "+", "frame": 1, "gene": "ruvA", "product": "Holliday junction branch migration protein RuvA", "db_xrefs": [ "COG:COG0632", "COG:L", "EC:3.6.4.12", "GO:0000400", "GO:0003678", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379", "GO:0036121", "GO:0048476", "GO:0061749", "GO:1990518", "SO:0001217", "UniRef:UniRef50_Q49424", "UniRef:UniRef90_Q49424" ], "nt": "TTGGAAAAAAATAAGCAAATTTTTTGTCATATTATTAAAAAAATTGTCGCTAACCAAATTATAGAAGAGGCTTTTGCCTTTAATACTCTAGAAGAAAAAGAGTGGTTCTGTAGATTAATAGAACTCAATGGTATTGGTAGTAAAACTGCACTTAATTTGCTCAATAATGACCTTGAGGAAATTAAACAATACATTCTGGAAAATAACTACAGTGCATTATGTGGTATTAACGGTGTAAATAACAAAATAGCTCGTGCACTTTTATCACTTGAAATATTTGAAAAATCTGAAAATAATAAAAATATTAAAGGAGTTCAAGTTGCTGATGGTTATGATGAATTGTTTGAAACACTAAAGTCACTTGGTTACAAACAACAAGAAATTCAGGATGCACTAAAAATGATAGAAGTAAAACCTGATTTTGATATAAGTCAGTTAGTTGCAGAAGTAATTAAATTAATGTCTTTTAAGAATAATGAAATTACAAATAAAACCGCCTAA", "aa": "MEKNKQIFCHIIKKIVANQIIEEAFAFNTLEEKEWFCRLIELNGIGSKTALNLLNNDLEEIKQYILENNYSALCGINGVNNKIARALLSLEIFEKSENNKNIKGVQVADGYDELFETLKSLGYKQQEIQDALKMIEVKPDFDISQLVAEVIKLMSFKNNEITNKTA", "aa_hexdigest": "914cde5cfa7e1f2a78d3a2c8ce195e3d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q49424", "query_cov": 1.0, "subject_cov": 0.8097560975609757, "identity": 0.9940000000000001, "score": 323.0, "evalue": 4.61e-111, "valid": true, "gene": "ruvA", "product": "Holliday junction branch migration protein RuvA", "ec_ids": [ "3.6.4.12" ], "uniref50_id": "UniRef50_Q49424", "cog_id": "COG0632", "cog_category": "L", "go_ids": [ "GO:0000400", "GO:0003678", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379", "GO:0036121", "GO:0048476", "GO:0061749", "GO:1990518" ] }, "pscc": { "uniref50_id": "UniRef50_Q49424", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49424" ], "product": "Holliday junction branch migration complex subunit RuvA" }, "genes": [ "ruvA" ], "id": "KMADJIBOEG_835", "locus": "GCA000292445_00834" }, { "type": "cds", "sequence": "contig_1", "start": 456797, "stop": 457720, "strand": "+", "frame": 2, "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "db_xrefs": [ "COG:COG2255", "COG:L", "EC:3.6.4.-", "EC:3.6.4.12", "GO:0000400", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0016887", "GO:0036121", "GO:0048476", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990518", "RefSeq:WP_009885816.1", "SO:0001217", "UniParc:UPI0000135382", "UniRef:UniRef100_Q49425", "UniRef:UniRef50_Q49425", "UniRef:UniRef90_Q49425" ], "nt": "ATGAAATTACAAATAAAACCGCCTAATACCTTTGATGAATTTGTAGGAAAACAAGAAATAATTAGTCAAATTCAATTAAGTATTAAAGCATCTAAATTAAATAAAACACAACTAGATCATATCTTGTTATATGGCCCACCTGGTGTGGGTAAAACTACTTTAGCCAGATTAATAGCAAATGAATTGAAAACAAAGTTGCAAATTATTCAAGGTGGACATTTACAAAAACCAAGCGATTTCTTAAACGCAATTTCACTCATTAAAAAAGGTGATGTTCTTTTTATAGATGAGATCCATGCCGTAGCACCTAATGTCATGGAACTAATGTATCCAGTTATGGATGTGTTCAAAATACAAGTATTAATTGGCAAGGATTTTAATTCCAAGATAGTTGAAATGAAGGTAAATCCTTTTACTCTAATTGGTGCAACTACACAACTTGGTAAAATCATCAATCCTTTAGAAGATAGATTTGGCGTTATCTTAAACATTAACTATTATTCAAATGCTGAAATTGAAAAGATGGTAAGTATCTATGGAAAGCAAATGAAGTTAGAGCTAAATTCAAATGAAATTTCAGCTATCACTGAACATAGTAAACAAACACCAAGAATTGCAATTAGAATAGTTAGAAGAATATTTGAACAAAAAATTGTTAATAAAAAAATAGACCTTGAGGGTTTGTTTAAGAATTTAATGATTTATAAAAATGGTCTGCAAAGTATTGATGTCCAATATCTTGAGGTTTTAAATCGCCAAAATGAACCACAAGGAATTAAGTCAATTAGTTCCATGTTAGGTATAGACAGACACACTATAGAAAATAAAATTGAACCTTTTTTGTTGCGTGAAAATATGATTCAAAAAACCAAAAAAGGCAGGATTATTACAAATAGCGGAAGAGAATATTTAGTTAACTTTTAA", "aa": "MKLQIKPPNTFDEFVGKQEIISQIQLSIKASKLNKTQLDHILLYGPPGVGKTTLARLIANELKTKLQIIQGGHLQKPSDFLNAISLIKKGDVLFIDEIHAVAPNVMELMYPVMDVFKIQVLIGKDFNSKIVEMKVNPFTLIGATTQLGKIINPLEDRFGVILNINYYSNAEIEKMVSIYGKQMKLELNSNEISAITEHSKQTPRIAIRIVRRIFEQKIVNKKIDLEGLFKNLMIYKNGLQSIDVQYLEVLNRQNEPQGIKSISSMLGIDRHTIENKIEPFLLRENMIQKTKKGRIITNSGREYLVNF", "aa_hexdigest": "029e8b1d2ad1ef50aa3ed7b7ed65658a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000135382", "ncbi_nrp_id": "WP_009885816.1", "uniref100_id": "UniRef100_Q49425", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000135382", "RefSeq:WP_009885816.1", "UniRef:UniRef100_Q49425" ] }, "ips": { "uniref100_id": "UniRef100_Q49425", "uniref90_id": "UniRef90_Q49425" }, "psc": { "uniref90_id": "UniRef90_Q49425", "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "ec_ids": [ "3.6.4.-", "3.6.4.12" ], "uniref50_id": "UniRef50_Q49425", "cog_id": "COG2255", "cog_category": "L", "go_ids": [ "GO:0000400", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0016887", "GO:0036121", "GO:0048476", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990518" ] }, "pscc": { "uniref50_id": "UniRef50_Q49425", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49425" ], "product": "Holliday junction branch migration complex subunit RuvB" }, "genes": [ "ruvB" ], "id": "KMADJIBOEG_836", "locus": "GCA000292445_00835" }, { "type": "cds", "sequence": "contig_1", "start": 457710, "stop": 457997, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATATTCAAACGATTATAGAAAACTACTGTCAATAACAAAACGATTATTCAACAGACTATTAATTAATACTGAAAAGAACGTTCGATTAATAGGTATTAGTTTTTTTGATTTAAAAAAGATAGATACAGATGAAGGACAGAAAAAATCTTTATTTTATCAATTCATACCAAAATCAATTAGCAAATTAAGCGAAGAATCTTCACTTGATAAATTAATTTTTGATATTAATGAAAGTTTTGGTTTTGAAATTATTAAAAGAGCTAATAAGCTTAAAAGTTAA", "aa": "MKKYSNDYRKLLSITKRLFNRLLINTEKNVRLIGISFFDLKKIDTDEGQKKSLFYQFIPKSISKLSEESSLDKLIFDINESFGFEIIKRANKLKS", "aa_hexdigest": "187c5e3628d36d6c5a42578e9c0f0fd3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11227.981499999989, "isoelectric_point": 9.800693321228025 }, "id": "KMADJIBOEG_837", "locus": "GCA000292445_00836" }, { "type": "cds", "sequence": "contig_1", "start": 458382, "stop": 458945, "strand": "-", "frame": 2, "gene": "dinP", "product": "putative protein MG360", "db_xrefs": [ "COG:COG0389", "COG:L", "GO:0003684", "GO:0003887", "GO:0009432", "GO:0042276", "SO:0001217", "UniRef:UniRef50_P75241", "UniRef:UniRef90_Q49426" ], "nt": "ATGATTAACACCTTTACATATTTTGAACCAGAATATTTAATTGATAAAAATTTAATTTTTCTTTATTTTGATTTTGATGCTTTTTTTGCATCTGTTGAAGAATTAGAAAATCCAGAATTAGTTAATCAACCCTTAATAGTTGGTAATCGCTTTTCAAGAAGTGTAGTTTCAACTTGTAACTATGTTGCTCGTAGTTATGGAATTAGATCTGGAATGTCTATTTTAAAAGCATTAGAACTATGTCCAAATGCTATTTTTGCACATTCTAATTTTCGTAATTACAGAAAACATTCTAAACGTATTTTTTCAGTTATTGAAAGCACTTTTTCATTAAAAATTGATGTTCTTTCTGTTGATGAAGGGGTTGCTTGTTTTCAAAACATTTCATTTAAAAAGGCATTTTTAATAGCGAAAAAAATAAAAAATTTTGTTTTTCAAAACTTGAGAATCAAAATTTCAATTGGCATCTCAGATCATTTTTTAATTGCTAAGATTTTTTCAAATCAAGCAAAACCATTTGGAATTAAAAGTTGTTCAGTTAAGGATATTAAAAAAAAACTTTGA", "aa": "MINTFTYFEPEYLIDKNLIFLYFDFDAFFASVEELENPELVNQPLIVGNRFSRSVVSTCNYVARSYGIRSGMSILKALELCPNAIFAHSNFRNYRKHSKRIFSVIESTFSLKIDVLSVDEGVACFQNISFKKAFLIAKKIKNFVFQNLRIKISIGISDHFLIAKIFSNQAKPFGIKSCSVKDIKKKL", "aa_hexdigest": "d6153e2b814a3478ebb588cb1d487661", "start_type": "ATG", "rbs_motif": null, "genes": [ "dinP" ], "truncated": "5-prime", "pseudogene": { "start": 458079, "stop": 458945, "observations": { "mutation": [ 458213, 458246, 458384 ], "stop": [ 458213, 458246, 458384 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49426", "query_cov": 1.0, "subject_cov": 0.45498783454987834, "identity": 1.0, "score": 367.0, "evalue": 5.43e-125, "gene_start": 1, "gene_end": 187, "reference_start": 1, "reference_end": 187, "reference_sequence": "MINTFTYFEPEYLIDKNLIFLYFDFDAFFASVEELENPELVNQPLIVGNRFSRSVVSTCNYVARSYGIRSGMSILKALELCPNAIFAHSNFRNYRKHSKRIFSVIESTFSLKIDVLSVDEGVACFQNISFKKAFLIAKKIKNFVFQNLRIKISIGISDHFLIAKIFSNQAKPFGIKSCSVKDIKKKLWPLPITEIPGIGEKHIDLVFKNNFYKINDLAVCEDASLLKKVFGNFWESLKAVSLGKWYTDNNNQVKSRSFAVSETLEDLNYSNNQLNKKLTQIFDQLFIRLQLSSQVCKGIVVQLKSNDFIVNSHSNKMKKYSNDYRKLLSITKRLFNRLLINTEKNVRLIGISFFDLKKIDTDEGQKKSLFYQFIPKSISKLSEESSLDKLIFDINESFGFEIIKRANKLKS" }, "paralog": false, "identity": 0.9895833333333334, "score": 558.0, "evalue": 5.4e-203, "description": "Internal stop codon at 458213, 458246, 458384. Nonsense mutation around 458213, 458246, 458384.", "psc": { "uniref90_id": "UniRef90_Q49426", "gene": "DinP", "product": "Uncharacterized protein MG360", "uniref50_id": "UniRef50_P75241", "cog_id": "COG0389", "cog_category": "L", "go_ids": [ "GO:0003684", "GO:0003887", "GO:0042276", "GO:0009432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49426", "UniRef:UniRef50_P75241", "COG:COG0389", "COG:L", "GO:0003684", "GO:0003887", "GO:0042276", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P75241", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75241" ], "product": "Uncharacterized protein MG360 homolog" } }, "id": "KMADJIBOEG_838", "locus": "GCA000292445_00837" }, { "type": "cds", "sequence": "contig_1", "start": 459065, "stop": 459553, "strand": "+", "frame": 2, "gene": "rplJ", "product": "50S ribosomal protein L10", "db_xrefs": [ "COG:COG0244", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0022625", "GO:0070180", "KEGG:K02864", "RefSeq:WP_009885818.1", "SO:0001217", "UniParc:UPI0000133B39", "UniRef:UniRef100_P36263", "UniRef:UniRef50_P36263", "UniRef:UniRef90_P36263" ], "nt": "ATGGTAGATAGTAAGAAAAATAAAAAACAGCAGGTTACGGATTTTTCTAATTTACTCTCTCAAAGTAAAGGATTTGTTATTTTTGACTATTCAGGAATGTCTGCTGTTGATGCAACTTTAATGAGAAAAAAGTTGTTTAATAAGGGTAGTAAGATAAAAATTGTTAAAAACAATATCTTAAGACGTGCTTTAAAAACTAGTAATTTTGAAGGTGTTGATGAATCGGTCATCAAAGGAAAAATTGCAGTTGCTGTTGGTATTAACGAGATCTTAGAAACCTTAAAAGTTGTTGATAGTGTAGTTAAAGAAAAAGAGTTAATGAAATTTGTTTGTGGTCATTTTGATAATCGTATTTTTAATAGTGATGACTTACAAAAAATAGCAAAACTCCCTGGTAGAAATGAACTTTATGGAATGTTTCTTTCAGTTCTACAAGCACCATTACGAAAATTTCTCTATGCTCTTCAGGCAGTAAGGAATGCTAAGTAA", "aa": "MVDSKKNKKQQVTDFSNLLSQSKGFVIFDYSGMSAVDATLMRKKLFNKGSKIKIVKNNILRRALKTSNFEGVDESVIKGKIAVAVGINEILETLKVVDSVVKEKELMKFVCGHFDNRIFNSDDLQKIAKLPGRNELYGMFLSVLQAPLRKFLYALQAVRNAK", "aa_hexdigest": "4d14f11ca59c4d52f196969e98ce2e5e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133B39", "ncbi_nrp_id": "WP_009885818.1", "uniref100_id": "UniRef100_P36263", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133B39", "RefSeq:WP_009885818.1", "UniRef:UniRef100_P36263" ] }, "ips": { "uniref100_id": "UniRef100_P36263", "uniref90_id": "UniRef90_P36263" }, "psc": { "uniref90_id": "UniRef90_P36263", "gene": "rplJ", "product": "50S ribosomal protein L10", "uniref50_id": "UniRef50_P36263", "cog_id": "COG0244", "cog_category": "J", "kegg_orthology_id": "K02864", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0022625", "GO:0070180" ] }, "pscc": { "uniref50_id": "UniRef50_P36263", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P36263" ], "product": "Large ribosomal subunit protein uL10" }, "genes": [ "rplJ" ], "id": "KMADJIBOEG_839", "locus": "GCA000292445_00838" }, { "type": "cds", "sequence": "contig_1", "start": 459576, "stop": 459944, "strand": "+", "frame": 3, "gene": "rplL", "product": "50S ribosomal protein L7/L12", "db_xrefs": [ "COG:COG0222", "COG:J", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "RefSeq:WP_009885819.1", "SO:0001217", "UniParc:UPI00001341B3", "UniRef:UniRef100_P36255", "UniRef:UniRef50_P36255", "UniRef:UniRef90_P36255" ], "nt": "ATGGGAAAACTAGATAAAAAACAATTAATTGAATCTCTAAAAGAGATGACTATAGTTGAAATTGATGAAATAATCAAGGCTGTTGAAGAAGCATTTGGTGTAACTGCAACTCCAATAGTAGCTGCTGGCGCAGCTGGTGCTACACAAGAAGCTGCTAGCGAAGTTAGTGTAAAGGTAACAGGATATGCTGATAATGCTAAGTTAGCTGTTTTAAAACTTTATCGTGAAATTACTGGAGTTGGTTTAATGGAAGCTAAAACTGCAGTTGAAAAATTACCTTGCGTTGTAAAACAAGATATTAAACCAGAAGAAGCAGAAGAACTTAAAAAGCGTTTTGTTGAAGTTGGTGCAACTGTTGAAGTTAAATAA", "aa": "MGKLDKKQLIESLKEMTIVEIDEIIKAVEEAFGVTATPIVAAGAAGATQEAASEVSVKVTGYADNAKLAVLKLYREITGVGLMEAKTAVEKLPCVVKQDIKPEEAEELKKRFVEVGATVEVK", "aa_hexdigest": "2a89c681ac8f1bc6764b695478e7c7bb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001341B3", "ncbi_nrp_id": "WP_009885819.1", "uniref100_id": "UniRef100_P36255", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001341B3", "RefSeq:WP_009885819.1", "UniRef:UniRef100_P36255" ] }, "ips": { "uniref100_id": "UniRef100_P36255", "uniref90_id": "UniRef90_P36255" }, "psc": { "uniref90_id": "UniRef90_P36255", "gene": "rplL", "product": "50S ribosomal protein L7/L12", "uniref50_id": "UniRef50_P36255", "cog_id": "COG0222", "cog_category": "J", "go_ids": [ "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P36255", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P36255" ], "product": "Large ribosomal subunit protein bL12" }, "genes": [ "rplL" ], "id": "KMADJIBOEG_840", "locus": "GCA000292445_00839" }, { "type": "cds", "sequence": "contig_1", "start": 459947, "stop": 460120, "strand": "+", "frame": 2, "gene": "rpmF", "product": "50S ribosomal protein L32", "db_xrefs": [ "COG:COG0333", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "RefSeq:WP_009885820.1", "SO:0001217", "UniParc:UPI0000165F83", "UniRef:UniRef100_P47603", "UniRef:UniRef50_P47603", "UniRef:UniRef90_P47603" ], "nt": "ATGGCAGTACAACAACGGCGTTCTAGTAAACACCGTCGTGATAAAAGACGTTCTCACGATGCACTTACTCTACAAACTTTAAGTGTTTGTAAGAAATGTGGTAAGAAGAAGTTATCACATCGTGTGTGCTCTTGTGGTATGTACGGTGAACTAAGAGTTAAAAAAGCTCACTAA", "aa": "MAVQQRRSSKHRRDKRRSHDALTLQTLSVCKKCGKKKLSHRVCSCGMYGELRVKKAH", "aa_hexdigest": "cf34ba30d94ac5e4214a0fce93736145", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000165F83", "ncbi_nrp_id": "WP_009885820.1", "uniref100_id": "UniRef100_P47603", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000165F83", "RefSeq:WP_009885820.1", "UniRef:UniRef100_P47603" ] }, "ips": { "uniref100_id": "UniRef100_P47603", "uniref90_id": "UniRef90_P47603" }, "psc": { "uniref90_id": "UniRef90_P47603", "gene": "rpmF", "product": "50S ribosomal protein L32", "uniref50_id": "UniRef50_P47603", "cog_id": "COG0333", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_P47603", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47603" ], "product": "Large ribosomal subunit protein bL32" }, "genes": [ "rpmF" ], "id": "KMADJIBOEG_841", "locus": "GCA000292445_00840" }, { "type": "cds", "sequence": "contig_1", "start": 460135, "stop": 460401, "strand": "-", "frame": 1, "gene": "rpsT", "product": "30S ribosomal protein S20", "db_xrefs": [ "COG:COG0268", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02968", "RefSeq:WP_009885821.1", "SO:0001217", "UniParc:UPI0000134E49", "UniRef:UniRef100_P55750", "UniRef:UniRef50_P55750", "UniRef:UniRef90_P55750" ], "nt": "ATGGCTAATATTAAATCTAACGAAAAACGATTACGTCAAGACATTAAGAGAAATTTAAATAATAAAGGACAAAAAACTAAACTAAAAACTAATGTTAAAAAATTTAATAAAGAGATTAATTTAGATAATCTCAGTTCTGTTTATTCTCAAGCAGATCGTTTAGCCAGAAAAGGGATTATTTCTTTAAACAGAGCTAAGCGTTTAAAATCAAAAAATGCTGTTATTTTGCATAAAAGTAATACAAATTCAACTGCAAAAAAACAATAA", "aa": "MANIKSNEKRLRQDIKRNLNNKGQKTKLKTNVKKFNKEINLDNLSSVYSQADRLARKGIISLNRAKRLKSKNAVILHKSNTNSTAKKQ", "aa_hexdigest": "5720b3c8f1e572f2bd77a0be8e9a894d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134E49", "ncbi_nrp_id": "WP_009885821.1", "uniref100_id": "UniRef100_P55750", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134E49", "RefSeq:WP_009885821.1", "UniRef:UniRef100_P55750" ] }, "ips": { "uniref100_id": "UniRef100_P55750", "uniref90_id": "UniRef90_P55750" }, "psc": { "uniref90_id": "UniRef90_P55750", "gene": "rpsT", "product": "30S ribosomal protein S20", "uniref50_id": "UniRef50_P55750", "cog_id": "COG0268", "cog_category": "J", "kegg_orthology_id": "K02968", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P55750", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P55750" ], "product": "Small ribosomal subunit protein bS20" }, "genes": [ "rpsT" ], "id": "KMADJIBOEG_842", "locus": "GCA000292445_00841" }, { "type": "cds", "sequence": "contig_1", "start": 460465, "stop": 460620, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGGTGGACAACAAGGGAGTTTTTTTGGGCTTTTAGTAATTGTTATTCCAATAATTTTGTTAATTGTTTTTTTTTCCAAGAAAAAAGGGGCACAAAAAAATGATTTTAGTGGTGAAGGAGGTAATCGATCATCAAGAAAAGATGAAGTGTGA", "aa": "MDGGQQGSFFGLLVIVIPIILLIVFFSKKKGAQKNDFSGEGGNRSSRKDEV", "aa_hexdigest": "c9c382566edc608eb2106c3ee9515271", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5529.328600000002, "isoelectric_point": 9.397443580627442 }, "id": "KMADJIBOEG_843", "locus": "GCA000292445_00842" }, { "type": "cds", "sequence": "contig_1", "start": 460639, "stop": 460995, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MG364", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47604", "UniRef:UniRef90_P47604" ], "nt": "TTGCAAGAAAAGAATGAACGTGGTAAAGAAATTATTAAAACTTTTGTAGCTAAAAAACCAAACCCTTTACATTCAAAAAAAGACCGCAAGCTTTTCAATCAAGAGATACAGGCATATATTACTAGTAATAACTTAGGAAAAAGCGAAGCAAAACGTTATAAAAATGAGCAAACTCGCTTAATGCAAAGAGAACTTTATTGTATTTATTTTGTTACAAAAGATGCTAAATCAACTGAAGTTGATGATGCTAGGATTATAGAAGCTGAGGTTTATCAAAAACCTACAAAAACCAAAAGTACTCCAGAGCGGCTAATTCGTATACTTGGTTTAAAAAATTTTGAAACTGAAATGCAATGA", "aa": "MQEKNERGKEIIKTFVAKKPNPLHSKKDRKLFNQEIQAYITSNNLGKSEAKRYKNEQTRLMQRELYCIYFVTKDAKSTEVDDARIIEAEVYQKPTKTKSTPERLIRILGLKNFETEMQ", "aa_hexdigest": "1f6d90f48589cd81fa683cff549a11aa", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 460465, "stop": 461139, "observations": { "mutation": [ 460618, 460993 ], "stop": [ 460618, 460993 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47604", "query_cov": 1.0, "subject_cov": 0.5267857142857143, "identity": 0.992, "score": 228.0, "evalue": 5.04e-74, "gene_start": 1, "gene_end": 118, "reference_start": 59, "reference_end": 176, "reference_sequence": "MDGGQQGSFFGLLVIVIPIILLIVFFSKKKGAQKNDFSGEGGNRSSRKDEVWKTIKQFLQEKNERGKEIIKTFVAKKPNPLHSKKDRKLFNQEIQAYITSNNLGKSEAKRYKNEQTRLMQRELYCIYFVTKDAKSTEVDDARIIEAEVYQKPTKTKSTPERLIRILGLKNFETEMQWIQPLMVREEKRKEKEEQKKLKLAARELKKKKKKKIKKPKEIRNQKNV" }, "paralog": false, "identity": 0.9866071428571429, "score": 341.0, "evalue": 2.86e-121, "description": "Internal stop codon at 460618, 460993. Nonsense mutation around 460618, 460993.", "psc": { "uniref90_id": "UniRef90_P47604", "product": "Uncharacterized protein MG364", "uniref50_id": "UniRef50_P47604", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47604", "UniRef:UniRef50_P47604" ] }, "pscc": { "uniref50_id": "UniRef50_P47604", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47604" ], "product": "Uncharacterized protein MG364" } }, "id": "KMADJIBOEG_844", "locus": "GCA000292445_00843" }, { "type": "cds", "sequence": "contig_1", "start": 461008, "stop": 461139, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTCGTGAAGAAAAGAGAAAAGAAAAAGAAGAACAGAAAAAACTTAAATTAGCTGCAAGAGAACTAAAAAAGAAGAAAAAGAAGAAAATAAAAAAACCAAAAGAAATCAGAAATCAGAAAAATGTTTAA", "aa": "MVREEKRKEKEEQKKLKLAARELKKKKKKKIKKPKEIRNQKNV", "aa_hexdigest": "fb7dc0fd268b06683a3ce1714668d93a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5344.465800000003, "isoelectric_point": 10.49488887786865 }, "id": "KMADJIBOEG_845", "locus": "GCA000292445_00844" }, { "type": "cds", "sequence": "contig_1", "start": 461132, "stop": 461497, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00551.24" ], "nt": "ATGTTTAAAATTGTTTTCTTTGGTACTTCAACGCTTTCAAAAAAATGTTTAGAACAACTTTTTTACGATAATGATTTTGAAATTTGTGCTGTTGTAACTCAGCCAGACAAAATTAATCATCGTAACAATAAAATAGTACCTTCTGATGTTAAGTCTTTTTGTTTGGAAAAAAACATAACTTTTTTTCAACCAAAACAAAGCATAAGCATAAAAGCTGATCTAGAAAAATTAAAAGCTGATATTGGTATTTGCGTTTCATTTGGCCAGTATCTTCATCAAGATATTATTGATCTTTTTCCAAATAAAGTAATTAACTTACATCCTTCTAAGTTACCACTACTTCGTGGTGGTGCACCATTACATTGA", "aa": "MFKIVFFGTSTLSKKCLEQLFYDNDFEICAVVTQPDKINHRNNKIVPSDVKSFCLEKNITFFQPKQSISIKADLEKLKADIGICVSFGQYLHQDIIDLFPNKVINLHPSKLPLLRGGAPLH", "aa_hexdigest": "905fc30a3e5ee055ff22e215af100c8d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00551.24", "name": "Formyl_trans_N", "length": 123, "aa_cov": 0.9752066115702479, "hmm_cov": 0.975609756097561, "evalue": 1.3589940834254818e-17, "score": 59.202640533447266, "start": 3, "stop": 120 } ], "seq_stats": { "molecular_weight": 13764.949899999981, "isoelectric_point": 8.693255424499512 }, "id": "KMADJIBOEG_846", "locus": "GCA000292445_00845" }, { "type": "cds", "sequence": "contig_1", "start": 462069, "stop": 463010, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00031D7E73", "UniRef:UniRef90_UPI00031D7E73" ], "nt": "ATGATTACAAAGAGTTTTTTCCTTAAAAATTTTGATCGCTCTAAAGAATTAATTCCTCTTTCATATAACGATGTTTTTAGCGCTGTTAATCAGTTTCTGAAAAGCTATACTGACGTTAATGATATTGATATTATTGAAGAAAATTTGATTGAAGATCCAGTTTTTGAACTTGATGTTTTAGAACTTTTAAACGAGGATCCAATGCTATTATTGGACAGTAAAAATCCCCGTGTTCAAGAAGCTAAAATTATTGCTAATAAAGCAAAAAAAAATATTAAAGATTATTTTAATCTTCCCATTTTTTTTGATACAGATAGTCTAGATAAAAATGTTTCTGTTTATCAAAAAGCTGAGCTTACTGAAAAGAAAATTCAAGAAATAATAACATCAAAAAAATCAGCTATTATTTTTAAACCTATTTTTGAAATAGAAGATTGCTTAATTCAACCAGATGCTATTATTGTTCATGAAAAAGGACTTTGTGAATTTGTTGTTATAAAAGCTACAACCAATACAAAAAGAAAATATTTTTTGGAAATAATTTATGACTTTGTGTTATTTAAAAAAATAGGTAAGTATAAACTTTTAAACTATTATTTTTGTACTGTTAAATACGAATTACAAAATAAAAATAATGTTTCTTTCTTTTTAAATACTGAAATAAAAACATCAAAAAACAGTTTTAGTTTAAGTTCAAAAGAAAAAGATTATTTTAAGAATAAACCTTTTAATCACCCTGAAAAAATTGCTTACATACACAAAAAAAAGAGCAATGGAGTTAATGGTTTTTTGATTGTAAAACTCATTGATAACCTTATTAAAAACAATATAGTTGATTTAAACAAAATAAGTGATTTTGTTACTAAAGAAATTGACAGTAAAAGTGTTCGTAATATTCAACCACTTATTAAAAATGCGGCTAAAATACAAATTAATTTTTGA", "aa": "MITKSFFLKNFDRSKELIPLSYNDVFSAVNQFLKSYTDVNDIDIIEENLIEDPVFELDVLELLNEDPMLLLDSKNPRVQEAKIIANKAKKNIKDYFNLPIFFDTDSLDKNVSVYQKAELTEKKIQEIITSKKSAIIFKPIFEIEDCLIQPDAIIVHEKGLCEFVVIKATTNTKRKYFLEIIYDFVLFKKIGKYKLLNYYFCTVKYELQNKNNVSFFLNTEIKTSKNSFSLSSKEKDYFKNKPFNHPEKIAYIHKKKSNGVNGFLIVKLIDNLIKNNIVDLNKISDFVTKEIDSKSVRNIQPLIKNAAKIQINF", "aa_hexdigest": "2e1dfd7af8b61c3537278b86f29a960c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 462069, "stop": 463313, "observations": { "mutation": [ 463008, 463185 ], "stop": [ 463008, 463185 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00031D7E73", "query_cov": 1.0, "subject_cov": 0.6879120879120879, "identity": 0.997, "score": 597.0, "evalue": 6.9e-213, "gene_start": 1, "gene_end": 313, "reference_start": 1, "reference_end": 313, "reference_sequence": "MITKSFFLKNFDRSKELIPLSYNDVFSAVNQFLKSYTDVNDIDIIEENLIEDPVFELDVLELLNEDPMLLLDSKNPRVQEAKIIANKAKKNIKDYFNLPIFFDTDSLDKNVSVYQKAELTEKKIQKIITSKKSAIIFKPIFEIEDCLIQPDAIIVHEKGLCEFVVIKATTNTKRKYFLEIIYDFVLFKKIGKYKLLNYYFCTVKYELQNKNNVSFFLNTEIKTSKNSFSLSSKEKDYFKNKPFNHPEKIAYIHKKKSNGVNGFLIVKLIDNLIKNNIVDLNKISDFVTKEIDSKSVRNIQPLIKNAAKIQINFWDQIQDIKKYQELKINQIVFNYSENFDSFWSNYLLRNLIKLVFAHKYNEIFKLSGKLANWSQLTYAYKENKSITINQLLHELNQKKSKANFNNSTNKISFFLEAWNSEKGFAIGNKFKNTWNKLKKRKFILILKQLVHQSES" }, "paralog": false, "identity": 0.9927536231884058, "score": 809.0, "evalue": 3.08e-299, "description": "Internal stop codon at 463008, 463185. Nonsense mutation around 463008, 463185.", "psc": { "uniref90_id": "UniRef90_UPI00031D7E73", "uniref50_id": "UniRef50_UPI00031D7E73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00031D7E73", "UniRef:UniRef50_UPI00031D7E73" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00031D7E73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00031D7E73" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 36505.9634, "isoelectric_point": 8.857198524475098 }, "id": "KMADJIBOEG_847", "locus": "GCA000292445_00846" }, { "type": "cds", "sequence": "contig_1", "start": 463664, "stop": 463945, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTACATTGATTAATGAACAAATATATAAAGAAAAAGTAAAAGCTATTGTTGACAATCTTTTTGACTTAGCTGATATTTTTACTATTGAAAATAACTGTTTGGCTTTCAAACAGCTAAACGGTTTTTCCTCTATCAAAAAGGTTTTAACTATTATAGATGAAAGCTTTTTAAAGGCAAGTAAATCGATTGGTTATCAAAATTTAAAAATCCAAAAAGGTGATGTAGCACAAGAAGTTGCTTTATCGCGTTTTCTAAATTGCTTAAATAAAAATGAATGA", "aa": "MATLINEQIYKEKVKAIVDNLFDLADIFTIENNCLAFKQLNGFSSIKKVLTIIDESFLKASKSIGYQNLKIQKGDVAQEVALSRFLNCLNKNE", "aa_hexdigest": "02ef597de60abf94d47f042022153718", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10515.09699999999, "isoelectric_point": 7.716768455505371 }, "id": "KMADJIBOEG_848", "locus": "GCA000292445_00847" }, { "type": "cds", "sequence": "contig_1", "start": 464095, "stop": 464217, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTCTTTTACAAGTTTTTTAAAAAGTAGTAAAGGAAGTCATTTTTTTAGTGATTTAAAAGAGAAGATAGAAAATCAAAAGATGTGTAAGAAACTAGCTATTAAACCTAAAAAAAATTAG", "aa": "MASFTSFLKSSKGSHFFSDLKEKIENQKMCKKLAIKPKKN", "aa_hexdigest": "57e6d5bc23b1c41667eb7dd61ca46cd1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4606.4355000000005, "isoelectric_point": 10.015501976013184 }, "id": "KMADJIBOEG_849", "locus": "GCA000292445_00848" }, { "type": "cds", "sequence": "contig_1", "start": 464278, "stop": 464574, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGCTAAAATTAGGTGATTTTGTTAAGTTAAGCAATGGTGCTGAACTAACTGAAAACACTGTTGGTGATGTACTTGAAGCTTTGGTTGGCGCCATTTATGAAGATATGGGGATGAAAAAAGCAACTGAATTTGTTGAAAAATATATCTTTGAAAGAACTTTTTCTGAGATTTTAAAATACGATTTCTTTTCACTTTTTCAAGAGCAAAAATTACCTGAACCAAGAGTTAGGGTAAGCTTAACTTCAAATAATTTGGTACTTAGTATAATTGAACTTGATGGTGATATTATTTGA", "aa": "MELKLGDFVKLSNGAELTENTVGDVLEALVGAIYEDMGMKKATEFVEKYIFERTFSEILKYDFFSLFQEQKLPEPRVRVSLTSNNLVLSIIELDGDII", "aa_hexdigest": "df634c168a522092a11f0653ccece72c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11154.69699999999, "isoelectric_point": 4.43170223236084 }, "id": "KMADJIBOEG_850", "locus": "GCA000292445_00849" }, { "type": "cds", "sequence": "contig_1", "start": 464873, "stop": 465859, "strand": "-", "frame": 3, "gene": "plsX", "product": "phosphate acyltransferase PlsX", "db_xrefs": [ "COG:COG0416", "COG:I", "EC:2.3.1.274", "GO:0005737", "GO:0006633", "GO:0008610", "GO:0008654", "GO:0043811", "KEGG:K03621", "RefSeq:WP_014894309.1", "SO:0001217", "UniParc:UPI00027B4199", "UniRef:UniRef100_UPI00027B4199", "UniRef:UniRef50_Q49427", "UniRef:UniRef90_Q49427" ], "nt": "ATGGCTTTTAGATTTGCAGTTGATTGTTTAGGTTTTGAAAACAAACCTAGTGAAGCAATTGAAGCAGTATTGAAATATTGGAGTTTTCATCAGGACTTAAATTTCATTCTGATCGGTGATGAAAAGGCTTTTGATGGTCTTGATATACTTCCAAAAAATATTACAAAAAAACTTGCTAATTCTTTCATTGAAATGACCGACACTCCACTAAGTGCAAGAAGAAAAGTTAACAGTTCAATGCAAATAGCCATAAACTTAGTTCGTGAAGGTAATGCTGATGTTGTAATTTCAGCAGGCTCTTCAGCAGTTTATGCTTCTTTAACAAATGATGCTTTTGGCAAAATTAATAAAAATACTAAGAGTGCTTTTATGTCTTATGTACCGACAGATAACAATAATTGGTTTTATTTTTTGGATGTTGGAGCTAATAAATATTTCACAGGAAAAGAACTTTATTTTTTAGGTTTAATGGCTGATATTTTTGTTAAAAAAACAACTACAAAAAAAACTCCTAAAATTGGACTATTGAATATAGGAACAGAAGAAAATAAAGGTTTTGATTATCATCAAGAAGCATTTACGCTTTTAAAAGCTGATAAAAATCTTAACTTTTTAGGATTTGTAGAATCAAGGTTTTTACTGGATGGAATATGTGATATTTTGATAGCTGATGGATATAGCGGCAATTTAGTTTTAAAAGCAATGGAAGGTACTTTTAAAACGATAGCACGGATTCTTAAGAGGGGTTATAAAAGAAATCCTCTTGCTGGCTTATTTAGCTTAGGTATTATCAAAAGTGTAGCCAAAAAATTTGATTACAAAAATAACGCTGGAGCTGTTGTAATGGGTTTAAATAAATTAGCTTTAAAAACTCACGGTTCTGCTGATAAACAACAATTTTTAAGCACGATTAGATTAGCACATCTAAGCTTAAAATCTGATCTTATTAATGCAATTAAAACTTCTTTGAATAACTATGAAGAATAA", "aa": "MAFRFAVDCLGFENKPSEAIEAVLKYWSFHQDLNFILIGDEKAFDGLDILPKNITKKLANSFIEMTDTPLSARRKVNSSMQIAINLVREGNADVVISAGSSAVYASLTNDAFGKINKNTKSAFMSYVPTDNNNWFYFLDVGANKYFTGKELYFLGLMADIFVKKTTTKKTPKIGLLNIGTEENKGFDYHQEAFTLLKADKNLNFLGFVESRFLLDGICDILIADGYSGNLVLKAMEGTFKTIARILKRGYKRNPLAGLFSLGIIKSVAKKFDYKNNAGAVVMGLNKLALKTHGSADKQQFLSTIRLAHLSLKSDLINAIKTSLNNYEE", "aa_hexdigest": "d865cf8aa4dc52ec7da7d863bf298c19", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B4199", "ncbi_nrp_id": "WP_014894309.1", "uniref100_id": "UniRef100_UPI00027B4199", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B4199", "RefSeq:WP_014894309.1", "UniRef:UniRef100_UPI00027B4199" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B4199", "uniref90_id": "UniRef90_Q49427" }, "psc": { "uniref90_id": "UniRef90_Q49427", "gene": "plsX", "product": "phosphate acyltransferase PlsX", "ec_ids": [ "2.3.1.274" ], "uniref50_id": "UniRef50_Q49427", "cog_id": "COG0416", "cog_category": "I", "kegg_orthology_id": "K03621", "go_ids": [ "GO:0005737", "GO:0006633", "GO:0008610", "GO:0008654", "GO:0043811" ] }, "pscc": { "uniref50_id": "UniRef50_Q49427", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49427" ], "product": "Phosphate acyltransferase" }, "genes": [ "plsX" ], "id": "KMADJIBOEG_851", "locus": "GCA000292445_00850" }, { "type": "cds", "sequence": "contig_1", "start": 465859, "stop": 467013, "strand": "-", "frame": 1, "gene": "fakA", "product": "putative protein MG369", "db_xrefs": [ "COG:COG1461", "COG:I", "GO:0004371", "GO:0006071", "SO:0001217", "UniRef:UniRef50_P75231", "UniRef:UniRef90_P47609" ], "nt": "GTGAAAAAAACTCCCCAAATGTTACCTGTATTAAAAGCATCAGGTGTGGTTGATTCAGGTGCTTATGGTTTTGCTTGTTTTCTTGAAGGAATGTTGTCATATTATGGGGGCGAATCTAATTTAGATGACAATACTTTATCAACTATAGAAATTAAATTTAACAAGTTTAAAGAACAACATGCTAATGAAGATGAATTTGGTTATTGTACAGAATATGTTTTAAGACTAGGTTTAAAAATAAATCAGACAGTTGAAAAGCAAAAATTTCATCAAAAAAAGTTTGAAAGTAAAGTAAATAGAATTGCCAATTCTGTAGTAATTGCTAGTGATAAAGACAATGGTTTTGTTAAAGTTCATGCTCATACTTTAAAACCACATCTTTTATTAGAAATGGGTCTTAATTATGGTGAATTTGAATTTGTCAAAATTGATAACATGAATTTACAGGTTAATAACAAGAATAACTCACCAACAAAAAGAGTTTTAAAACCTGCTATAGTAGCTACTGTACCTACAGAAGCATTTGCTGAAAGAATTCGTGAAGATCATGATATTAACGCCATATTATGTACTGATGATACTGGGGCTCCTTCTGTTTTTTCTTTACTTGAGGCTGTTAAGTTAACCAATTCTAGTAATGTAATCTTTTTGTTACATGATAAAAACTATTTTCTATCTGCCAATGAAACAATTAAACAGTTAAAACACCAAAAGATTAATGCAGATTATGTTATTACAGCAAATCCTGTGGAGTCAATTGCTGCTTTAACTGTATTTAACAGTGATCTTTCTATTCACACTAATGTGAAAGCAATGAAGCGCTTTATTAAAGAGTTTGCTTCCGCTACGATAACACAAGCTTCAAAGTCATATAAAGAAAACAAAGTAATGGTGAATAAAAATGATTTTATTGCTGTTACAAATAAAAGTATTATTGCTAGTGAATCTCAACTTACAGACTGTTTTTTTAAAACAATTGATATCCTTTCAAAAAAAGTGAAAAAACCTGAGTTCCTTTTGGCTTATTATGGCAAAGACATCACTGAACAAGATGCGAAAAAAATGCAAGCTTTAGTTGAAAAAAAATATAAATTGTTTTGTGAATTTTCACCTGGAGAACAAAAAGTGTTCTCTTACATAATAGGCATACAGTAA", "aa": "MKKTPQMLPVLKASGVVDSGAYGFACFLEGMLSYYGGESNLDDNTLSTIEIKFNKFKEQHANEDEFGYCTEYVLRLGLKINQTVEKQKFHQKKFESKVNRIANSVVIASDKDNGFVKVHAHTLKPHLLLEMGLNYGEFEFVKIDNMNLQVNNKNNSPTKRVLKPAIVATVPTEAFAERIREDHDINAILCTDDTGAPSVFSLLEAVKLTNSSNVIFLLHDKNYFLSANETIKQLKHQKINADYVITANPVESIAALTVFNSDLSIHTNVKAMKRFIKEFASATITQASKSYKENKVMVNKNDFIAVTNKSIIASESQLTDCFFKTIDILSKKVKKPEFLLAYYGKDITEQDAKKMQALVEKKYKLFCEFSPGEQKVFSYIIGIQ", "aa_hexdigest": "941082cccee5a48c9a779484d694c9f6", "start_type": "GTG", "rbs_motif": null, "genes": [ "fakA" ], "truncated": "3-prime", "pseudogene": { "start": 465859, "stop": 467313, "observations": { "mutation": [ 467022 ], "stop": [ 467022 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47609", "query_cov": 1.0, "subject_cov": 0.6894075403949731, "identity": 0.997, "score": 752.0, "evalue": 2.22e-271, "gene_start": 1, "gene_end": 384, "reference_start": 174, "reference_end": 557, "reference_sequence": "MSSVNLSSFIDMLRLGCNNIAKNYEYINQLNVFPVPDGDTGTNMKVTITEAIKKLENEKSHIKSFSELGKNFTRDLLLFSRGNSGVIFSQIMKGFFSNIIVNKTSNNSELSIEDVANAFIVAQEVAYKNVSKPVEGTMLTVARLISNEFKSQKNRPKTLEKLFEQAVKVAWQAVKKTPQMLPVLKASGVVDSGAYGFACFLEGMLSYYGGESNLDDNTLSTIEIKFNKFKEQHANEDEFGYCTEYVLRLGLKINQTVEKQKFHQKKFESKVNRIANSVVIASDKDNGFVKVHAHTLKPHLLLEMGLNYGEFEFVKIDNMNLQVNNKNNSPTKRVLKPAIVATVPTEAFAERIREDHDINAILCTDDTGAPSVFSLLEAVKLTNSSNVIFLLHDKNYFLSANETIKQLKHQKINADYVITANPVESIAALTVFNSDLSIHTNVKAMKRFIKEFASATITQASKSYKENKVMVNKNDFIAVTNKSIIASESQLTDCFFKTIDILSKKVKKPEFLLAYYGKDITEQDAKKMQALVEKKYKLFCEFSPGEQKVFSYIIGIQ" }, "paralog": false, "identity": 0.9979338842975206, "score": 941.0, "evalue": 0.0, "description": "Internal stop codon at 467022. Nonsense mutation around 467022.", "psc": { "uniref90_id": "UniRef90_P47609", "gene": "FakA", "product": "Uncharacterized protein MG369", "uniref50_id": "UniRef50_P75231", "cog_id": "COG1461", "cog_category": "I", "go_ids": [ "GO:0004371", "GO:0006071" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47609", "UniRef:UniRef50_P75231", "COG:COG1461", "COG:I", "GO:0004371", "GO:0006071" ] }, "pscc": { "uniref50_id": "UniRef50_P75231", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75231" ], "product": "Uncharacterized protein MG369 homolog" } }, "id": "KMADJIBOEG_852", "locus": "GCA000292445_00851" }, { "type": "cds", "sequence": "contig_1", "start": 467020, "stop": 467499, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTACGCCTTGGCTGTAATAACATCGCTAAAAATTATGAATACATAAACCAGTTGAATGTTTTTCCTGTTCCTGATGGTGACACTGGAACAAACATGAAAGTAACAATAACAGAAGCCATCAAGAAACTAGAAAATGAAAAAAGTCATATAAAAAGCTTTAGTGAACTTGGTAAAAACTTTACTAGAGATTTACTTTTGTTTTCACGTGGCAATTCAGGGGTTATCTTTTCACAAATAATGAAAGGATTTTTCAGTAACATAATCGTAAATAAAACAAGCAATAATTCAGAGTTGAGTATAGAAGATGTTGCAAATGCATTCATTGTTGCTCAAGAAGTGGCTTATAAAAACGTATCAAAACCAGTAGAAGGAACTATGCTAACAGTTGCTCGTTTAATAAGCAATGAGTTTAAGAGTCAAAAAAATCGTCCTAAAACACTTGAAAAACTTTTTGAACAAGCTGTTAAAGTAGCTTGA", "aa": "MLRLGCNNIAKNYEYINQLNVFPVPDGDTGTNMKVTITEAIKKLENEKSHIKSFSELGKNFTRDLLLFSRGNSGVIFSQIMKGFFSNIIVNKTSNNSELSIEDVANAFIVAQEVAYKNVSKPVEGTMLTVARLISNEFKSQKNRPKTLEKLFEQAVKVA", "aa_hexdigest": "721325462c9e4b522b254cac3ff677ea", "start_type": "ATG", "rbs_motif": "ATTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17810.2875, "isoelectric_point": 9.325174522399902 }, "id": "KMADJIBOEG_853", "locus": "GCA000292445_00852" }, { "type": "cds", "sequence": "contig_1", "start": 467601, "stop": 467870, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTTTAATAACAATTAGATTAATTAGTGGTAGGACTCACCAAATTCGTGCGTGTTTAAATTTATTTTCAAATCAACTAGTTGGTGATAAAAAATACAGCTTGATTCAATTTAAAAATCGTAATAATAAATATAAGCATCAAGCACTTCACGCTTATGAATTAGTTTTTCCTAAACTAGAAACGAGCAAGTTTCCCATTTTAACAAATTATTCATTAATGCAATTCAAGAGTAAAATAGTACCTTGGTTTGAATATTTAATTCAATAG", "aa": "MSLITIRLISGRTHQIRACLNLFSNQLVGDKKYSLIQFKNRNNKYKHQALHAYELVFPKLETSKFPILTNYSLMQFKSKIVPWFEYLIQ", "aa_hexdigest": "e85fdb441f3f920480a09b477d5db21e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10607.384099999994, "isoelectric_point": 10.00628299713135 }, "id": "KMADJIBOEG_854", "locus": "GCA000292445_00853" }, { "type": "cds", "sequence": "contig_1", "start": 467979, "stop": 468572, "strand": "-", "frame": 2, "gene": "rluA", "product": "putative RNA pseudouridine synthase MG370", "db_xrefs": [ "COG:COG0564", "COG:J", "EC:5.4.99.-", "GO:0000455", "GO:0003723", "GO:0009982", "GO:0140098", "SO:0001217", "UniRef:UniRef50_P47610", "UniRef:UniRef90_P47610" ], "nt": "ATGAGAATAGCTAATAAATTTTTAGTTCCTAAAGAAAGTGAAAACCAACGAATTGACCAATTTTGTTTAAAAATCCTTCCCTTAATTAAAAGAAGTGACTTTTTTAAGTATTTACGCTTAGGAAAGGTTTTGTTAAATAAAACAAAACCACAGGTCAACACAAGATTAAAAACTAAAGATGAGATAGCTTTTCTTTTTGATATAAATCCCTATTTACAAACTAATAATGACTATTTATCTCTAGATTTGGTAAAAGATAAGCTAAAAATTATATTTGAGGATGAAAATATTATTGTAATTGACAAACCAACTGGAATAGTTTGCCAACCTGATAAAAAACATAGCATTATTAATTTATCAAATATGCTTTTAAAACACTGTGGATATAGACAATTTGACAGTAATAAACTTAACTTTTATCCCCAGTTTGCACATCGTATTGATCGTAATACTTCTGGAATTGTTATTGGTGCTAAAACAAACAAAGCTTTAAAAGAATTGAACAAAGTTTTTAAAAATAACCATTTAACCAAAAGATATAAAGGCTTAGTTTTTGGTCAATTTAACCATCTTGGCTTACAAACTGCTTATTGA", "aa": "MRIANKFLVPKESENQRIDQFCLKILPLIKRSDFFKYLRLGKVLLNKTKPQVNTRLKTKDEIAFLFDINPYLQTNNDYLSLDLVKDKLKIIFEDENIIVIDKPTGIVCQPDKKHSIINLSNMLLKHCGYRQFDSNKLNFYPQFAHRIDRNTSGIVIGAKTNKALKELNKVFKNNHLTKRYKGLVFGQFNHLGLQTAY", "aa_hexdigest": "5a39bc7eae998ed19166b7f29fbb4bd1", "start_type": "ATG", "rbs_motif": null, "genes": [ "rluA" ], "truncated": "5-prime", "pseudogene": { "start": 467676, "stop": 468572, "observations": { "mutation": [ 467942, 467981 ], "stop": [ 467942, 467981 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47610", "query_cov": 1.0, "subject_cov": 0.6099071207430341, "identity": 0.995, "score": 396.0, "evalue": 1.16e-137, "gene_start": 1, "gene_end": 197, "reference_start": 1, "reference_end": 197, "reference_sequence": "MRIANKFLVPKESENQRIDQFCLKILPLIKRSDFFKYLRLGKVLLNKTKPQVNTRLKTKDEIAFLFDINPYLQTNNDYLSLDLVKDKLKIIFEDENIIVVDKPTGIVCQPDKKHSIINLSNMLLKHCGYRQFDSNKLNFYPQFAHRIDRNTSGIVIGAKTNKALKELNKVFKNNHLTKRYKGLVFGQFNHLGLQTAYWKKDNNNGIVTVKWKPFPEAKKISTFFENSSYIAQKDMSLITIRLISGRTHQIRACLNLFSNQLVGDKKYSLIQFKNRNNKYKHQALHAYELVFPKLETSKFPILTNYSLMQFKSKIVPWFEYLIQ" }, "paralog": false, "identity": 0.9899328859060402, "score": 592.0, "evalue": 1.29e-217, "description": "Internal stop codon at 467942, 467981. Nonsense mutation around 467942, 467981.", "psc": { "uniref90_id": "UniRef90_P47610", "gene": "RluA", "product": "Uncharacterized RNA pseudouridine synthase MG370", "ec_ids": [ "5.4.99.-" ], "uniref50_id": "UniRef50_P47610", "cog_id": "COG0564", "cog_category": "J", "go_ids": [ "GO:0140098", "GO:0009982", "GO:0003723", "GO:0000455" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47610", "EC:5.4.99.-", "UniRef:UniRef50_P47610", "COG:COG0564", "COG:J", "GO:0140098", "GO:0009982", "GO:0003723", "GO:0000455" ] }, "pscc": { "uniref50_id": "UniRef50_P47610", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47610" ], "product": "Uncharacterized RNA pseudouridine synthase MG370" } }, "id": "KMADJIBOEG_855", "locus": "GCA000292445_00854" }, { "type": "cds", "sequence": "contig_1", "start": 468559, "stop": 468678, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCAATGGTGGTGGACATAAATATGCAGCTGCTTTTGTTTTGGATGAAAAAAATCAATTTATGAAATTAGTACAGATAATGGATGATTTTCTTGCCAAACAAAATGAGAATAGCTAA", "aa": "MFNGGGHKYAAAFVLDEKNQFMKLVQIMDDFLAKQNENS", "aa_hexdigest": "6349d3033f3deba959e08d9f64c10586", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4451.0236, "isoelectric_point": 5.498172187805174 }, "id": "KMADJIBOEG_856", "locus": "GCA000292445_00855" }, { "type": "cds", "sequence": "contig_1", "start": 468727, "stop": 468981, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAACTGGTTTTAACCGCAATAAGGTTCATGATGCAGTTTATTTAAAGCCATTACTAGAACATAAGTACTTTAGCTATGTACTTAGCAAAGCCAAAATAACTAAAAATGGGTTAGCTTATGCATTGATTAAAAAAGGAGCATATAAACATTTTGGTGTTGTCTCTCCTTTACCAATGGTTCATGCTTTGAATAACATAAAAGGAGTAAAAATATGGACTACTGTTTACTTTAACGAGTCCATTAAAAAATGA", "aa": "METGFNRNKVHDAVYLKPLLEHKYFSYVLSKAKITKNGLAYALIKKGAYKHFGVVSPLPMVHALNNIKGVKIWTTVYFNESIKK", "aa_hexdigest": "3973dcb71ca265a51be8444ae6032056", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9612.270499999997, "isoelectric_point": 9.953547859191893 }, "id": "KMADJIBOEG_857", "locus": "GCA000292445_00856" }, { "type": "cds", "sequence": "contig_1", "start": 469027, "stop": 469125, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGGTTATTTAATTTTGCAGATGGGTTACAAATTAAATGATGAGATTGCATCATATCTTTATGCAGGAATAATTACTGATACACAGCGATTCTGA", "aa": "MIGYLILQMGYKLNDEIASYLYAGIITDTQRF", "aa_hexdigest": "59cfb9f83911f8505c9aa190a1ea6976", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3715.294899999999, "isoelectric_point": 4.556577491760254 }, "id": "KMADJIBOEG_858", "locus": "GCA000292445_00857" }, { "type": "cds", "sequence": "contig_1", "start": 469156, "stop": 469533, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01368.27" ], "nt": "ATGATTAGTATCGATCCACAATTCATTAAGAATTTTTCAAAAAAGGTTAAACAGTTTGATAAGTTCAGTTTATTTGTACATGTAAACCCAGACTTTGATGCATTTGGATCTGCTTTTGCTTTCAAAACTTTCCTAAATACTTTTTTCAGTGAAAAAAAAGCTTATGTTATGGGTTCATACAATATTAATGCAGACGGTAGAGAACTTTTTCCTTTTGAGCAAACTGACATAAATGATGATTTTGTAAAAGAATCTTTAGCTATTATTTTTGATACTTCCAACCAAGAAAGAGTTTTAACTCAAAAACATAAGTTGGCAAAAGAGACAGTTAGGATAGATCACCATCCTAGAACTGAAAAATTTGCTGATATGGAATGA", "aa": "MISIDPQFIKNFSKKVKQFDKFSLFVHVNPDFDAFGSAFAFKTFLNTFFSEKKAYVMGSYNINADGRELFPFEQTDINDDFVKESLAIIFDTSNQERVLTQKHKLAKETVRIDHHPRTEKFADME", "aa_hexdigest": "4cfe512bd2c9cd15a27e88cd27053a61", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01368.27", "name": "DHH", "length": 100, "aa_cov": 0.784, "hmm_cov": 0.75, "evalue": 4.642164492421006e-11, "score": 38.24043655395508, "start": 24, "stop": 121 } ], "seq_stats": { "molecular_weight": 14628.388799999979, "isoelectric_point": 6.077304649353027 }, "id": "KMADJIBOEG_859", "locus": "GCA000292445_00858" }, { "type": "cds", "sequence": "contig_1", "start": 469526, "stop": 470464, "strand": "-", "frame": 3, "gene": "thiI", "product": "putative tRNA sulfurtransferase", "db_xrefs": [ "COG:COG0301", "COG:HJ", "EC:2.8.1.4", "GO:0000049", "GO:0002937", "GO:0004810", "GO:0005524", "GO:0005829", "GO:0009228", "GO:0009229", "GO:0052837", "GO:0140741", "KEGG:K03151", "SO:0001217", "UniRef:UniRef50_P47612", "UniRef:UniRef90_P47612" ], "nt": "TTGCCTGGAATTAGTTTGTTCTTTTTTGCATCACAGATAGTAAGGGAAGAAAACAAACTATTAGATCTTCTTTTTAATTTATTTAAAGATTTTAATTCCTTTAAATTAGAAGTTAAGAGACGTGATAAAAATTTTGCTGAAAATTCAAGTAATTTTAAGAAGTATTTAGCAGTTAAACTATTTGAAAAATACCAATTAAAAGGAGTAATTAATAACCCTGAAATAATAGCTAATATAGAGATTTTGAAGGAGCATTTTCTTGTTTTTACAGAACGTTTTAAAGGTAAAGGTGGATTACCTGTTTATAGTAGTGGTAAAGCTTTAGTCTTACTTTCAGGAGGAATTGATAGCCCAGTTGCAGCTTCACTTGTAATGCAACGTGGTTTTAACATTGATTTTATCACCTTTATTAATGAACCAAATAAGAATCAAAAAACAATTGAAAAAATCACAAGGTTAGCAAATTTAATTTCTTTTAATAAAACGATTTGTAGTGGTAAATTATTAGTTTTTGATTTCACTGCAATTCAAAAAGAGTTAATTCATATTAGTAATGAATCTTATCGTATCGTTTTAATGCGTCGTGTTTTTTATAAAGCTGCTAGTATGTTTAAGTATGATTGTCTAGTTACAGGTGAGGTTTTGGGACAAGTTGCATCCCAAACCATTGAAAATTTAAAAGTTATTCAATCAGCAACACCAGATACATTTATAGTTCGCCCCTTAATTGGCTTTAGTAAAGATAAAATTATTGAATTAGCTAAATTTTTTAATACTTTTGATATTTCTATTGAACAACATTTAGATACTTGTAGTGAGTTTTCTCCCAAAAATCCTACAACGAAAGCTAAATTGATAAATGTTGAAAAGCTTGAAAGTGAGCTTATTTTTTTAAATGATTTAATTGAAAAGGGAGTAAGTGAATTAAGCAATGATTAG", "aa": "MPGISLFFFASQIVREENKLLDLLFNLFKDFNSFKLEVKRRDKNFAENSSNFKKYLAVKLFEKYQLKGVINNPEIIANIEILKEHFLVFTERFKGKGGLPVYSSGKALVLLSGGIDSPVAASLVMQRGFNIDFITFINEPNKNQKTIEKITRLANLISFNKTICSGKLLVFDFTAIQKELIHISNESYRIVLMRRVFYKAASMFKYDCLVTGEVLGQVASQTIENLKVIQSATPDTFIVRPLIGFSKDKIIELAKFFNTFDISIEQHLDTCSEFSPKNPTTKAKLINVEKLESELIFLNDLIEKGVSELSND", "aa_hexdigest": "06acc8cba75c9f2814e4cebc0a5f041d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47612", "query_cov": 1.0, "subject_cov": 0.8103896103896104, "identity": 0.997, "score": 601.0, "evalue": 1.1e-215, "valid": true, "gene": "thiI", "product": "Probable tRNA sulfurtransferase", "ec_ids": [ "2.8.1.4" ], "uniref50_id": "UniRef50_P47612", "cog_id": "COG0301", "cog_category": "HJ", "kegg_orthology_id": "K03151", "go_ids": [ "GO:0005829", "GO:0005524", "GO:0004810", "GO:0000049", "GO:0140741", "GO:0009228", "GO:0009229", "GO:0052837", "GO:0002937" ] }, "pscc": { "uniref50_id": "UniRef50_P47612", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47612" ], "product": "Probable tRNA sulfurtransferase" }, "genes": [ "thiI" ], "id": "KMADJIBOEG_860", "locus": "GCA000292445_00859" }, { "type": "cds", "sequence": "contig_1", "start": 470593, "stop": 470682, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATTGAATAGTGAAGATGTATTAGTTGCGCGTTATGGTGAATTGGTTTTAAAAGGAAAAACCGTTCTTATTTCACAAAACAATTAA", "aa": "MELNSEDVLVARYGELVLKGKTVLISQNN", "aa_hexdigest": "8b0bf45ee26cd47e8ed82ec0c0127588", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3233.689800000001, "isoelectric_point": 4.867827796936035 }, "id": "KMADJIBOEG_861", "locus": "GCA000292445_00860" }, { "type": "cds", "sequence": "contig_1", "start": 470753, "stop": 470914, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGCAATGTAAATAAAGGACTTTTTGCAATTGCCTTTTTGAAACCAGAAGATTATGTCTTACCAGAACAATTTAATGCTAGCAATAGAGAAATTAGGTTAGTTCCTATCAAGATAGATCATAAGGGATTTAGTGTTTTAGTTGATAAGGCGAGAATTTGA", "aa": "MRNVNKGLFAIAFLKPEDYVLPEQFNASNREIRLVPIKIDHKGFSVLVDKARI", "aa_hexdigest": "774c4502fc051e890c4788580664c866", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6112.1125, "isoelectric_point": 9.693288993835449 }, "id": "KMADJIBOEG_862", "locus": "GCA000292445_00861" }, { "type": "cds", "sequence": "contig_1", "start": 470981, "stop": 471340, "strand": "-", "frame": 3, "gene": null, "product": "MPN551 family DNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75227", "UniRef:UniRef90_P47613" ], "nt": "ATGGTCAAAATTTATGAAGATGAATTTGATGAAACACTGGCAAAAGCTGGAAACATAATAGAACCTAAAATACGTGATTATGTCAATTTAAAAACTGGTTTTAATTTTCATTCTTATGATCCTAAAAAAGTTCAATTTGATTTATTTAAGGATGATAGTGTTTTTGGTGGGATACCTGATGGTGAACCTCTTGATGAAAATGGTGAATTAGCTTATCAAAATGATCTTCCTATGTTAGAGATTAAAACAACTTCTTGCGATAGTCTTATTTATAAAAAAATCAATGGAAATCTCAAGATGATTCTTGATGAAAATGGTATGCCTATTGTTAAAAAGAAAGATGGCAAAAAAGATAGTTGA", "aa": "MVKIYEDEFDETLAKAGNIIEPKIRDYVNLKTGFNFHSYDPKKVQFDLFKDDSVFGGIPDGEPLDENGELAYQNDLPMLEIKTTSCDSLIYKKINGNLKMILDENGMPIVKKKDGKKDS", "aa_hexdigest": "c7c7b9ad22adf906217200395552bd5b", "start_type": "ATG", "rbs_motif": "ATTTA", "genes": [], "truncated": "both", "pseudogene": { "start": 470678, "stop": 471514, "observations": { "mutation": [ 470695, 470755, 470983, 471349 ], "stop": [ 470695, 470755, 470983, 471349 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47613", "query_cov": 1.0, "subject_cov": 0.4234875444839858, "identity": 1.0, "score": 242.0, "evalue": 5.48e-79, "gene_start": 1, "gene_end": 119, "reference_start": 59, "reference_end": 177, "reference_sequence": "MGFIKQYKTDFDIVDNQIVLSEQYFLRNKALFKKITGTRFGKVLGLSEYESSFKTWANMVKIYEDEFDETLAKAGNIIEPKIRDYVNLKTGFNFHSYDPKKVQFDLFKDDSVFGGIPDGEPLDENGELAYQNDLPMLEIKTTSCDSLIYKKINGNLKMILDENGMPIVKKKDGKKDSWFDSNGKIIISTLYYCQIGLYLYLRNVNKGLFAIAFLKPEDYVLPEQFNASNREIRLIPIKIDHKGFSVLVDKARIWYENYILTGKSPKLTESDIQWLKENGIE" }, "paralog": false, "identity": 0.9820143884892086, "score": 545.0, "evalue": 9.76e-201, "description": "Internal stop codon at 470695, 470755, 470983, 471349. Nonsense mutation around 470695, 470755, 470983, 471349.", "psc": { "uniref90_id": "UniRef90_P47613", "product": "MPN551 family DNA-binding protein", "uniref50_id": "UniRef50_P75227", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47613", "UniRef:UniRef50_P75227" ] }, "pscc": { "uniref50_id": "UniRef50_P75227", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75227" ], "product": "Uncharacterized protein MG373 homolog" } }, "id": "KMADJIBOEG_863", "locus": "GCA000292445_00862" }, { "type": "cds", "sequence": "contig_1", "start": 471347, "stop": 471514, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGATTTATAAAGCAATATAAAACTGATTTTGATATAGTTGATAATCAAATTGTTCTATCAGAACAGTATTTTTTAAGAAATAAAGCTCTATTTAAAAAAATAACCGGTACTCGCTTTGGCAAGGTTTTAGGTTTATCTGAATATGAGTCATCATTTAAAACTTGA", "aa": "MGFIKQYKTDFDIVDNQIVLSEQYFLRNKALFKKITGTRFGKVLGLSEYESSFKT", "aa_hexdigest": "7517bb11e32be49a605a3309da56ba93", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6444.3674999999985, "isoelectric_point": 9.397701454162597 }, "id": "KMADJIBOEG_864", "locus": "GCA000292445_00863" }, { "type": "cds", "sequence": "contig_1", "start": 471515, "stop": 471871, "strand": "-", "frame": 3, "gene": null, "product": "MPN552 family protein", "db_xrefs": [ "GO:0005524", "SO:0001217", "UniRef:UniRef50_P75226", "UniRef:UniRef90_P47614" ], "nt": "ATGGATGTTAGTTCTATTTATGTATTTAGTCCTCAGTATCATTTAGACTTTCAACTTATGGAACATATTTCTAGTGGTCAGCCTAAGTTTCATTACAGCAATTTTTATAACGAAAATTTTTTATTTACTAGCACAAAAAATTCCAAGTTTAGTGCTTTAGAAACATATGTTAAAAGCAAGAATTGCTCTTTAAAAGATGTTCATTATTTAAATGTGACAGAAGTTCCAATTAATAACATTAATCAATTAGCAAGTGTTGTATTTTTAAGCAAAAAACAACCAGATAATGATGAGGAATTTAATAATCCTGAAATATCAATTGTTTTAAAAGGAATTTGTGAACAGTTGATGAAATAA", "aa": "MDVSSIYVFSPQYHLDFQLMEHISSGQPKFHYSNFYNENFLFTSTKNSKFSALETYVKSKNCSLKDVHYLNVTEVPINNINQLASVVFLSKKQPDNDEEFNNPEISIVLKGICEQLMK", "aa_hexdigest": "bc1bb6186708604d079a0f72d40bf52e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 471515, "stop": 472171, "observations": { "mutation": [ 471931, 472123 ], "stop": [ 471931, 472123 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47614", "query_cov": 1.0, "subject_cov": 0.4306569343065693, "identity": 1.0, "score": 236.0, "evalue": 7.99e-77, "gene_start": 1, "gene_end": 118, "reference_start": 157, "reference_end": 274, "reference_sequence": "MDRKIVVLDINTANFFNTTKDLEVWKNFFNFIQANNHQLVFMSSCWQQAVLYLLDLLSLDDVDIIAESGAIIWISKTNEFIYQSFLDSVSIDTIVHHAIITNSGVFAIGKSKISEEPNTTINYFISLEKYKQFKLIWLTEFDQTLKYQNFLKNLSEMDVSSIYVFSPQYHLDFQLMEHISSGQPKFHYSNFYNENFLFTSTKNSKFSALETYVKSKNCSLKDVHYLNVTEVPINNINQLASVVFLSKKQPDNDEEFNNPEISIVLKGICEQLMK" }, "paralog": false, "identity": 0.9908256880733946, "score": 426.0, "evalue": 5.86e-154, "description": "Internal stop codon at 471931, 472123. Nonsense mutation around 471931, 472123.", "psc": { "uniref90_id": "UniRef90_P47614", "product": "MPN552 family protein", "uniref50_id": "UniRef50_P75226", "go_ids": [ "GO:0005524" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47614", "UniRef:UniRef50_P75226", "GO:0005524" ] }, "pscc": { "uniref50_id": "UniRef50_P75226", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75226" ], "product": "Uncharacterized protein MG374 homolog" } }, "id": "KMADJIBOEG_865", "locus": "GCA000292445_00864" }, { "type": "cds", "sequence": "contig_1", "start": 472265, "stop": 472393, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAAAATCACCAGAATTAGCTTTAATAATTTTGTTAAAAGAGTTAGAAGAGATGGATAGAAAAATAGTTGTTTTAGATATTAATACAGCAAATTTTTTTAACACTACAAAGGATTTAGAAGTATGA", "aa": "MKKSPELALIILLKELEEMDRKIVVLDINTANFFNTTKDLEV", "aa_hexdigest": "f87a55e59762405bae8c3c7f2ba4f7fe", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4876.728700000001, "isoelectric_point": 4.8126939773559565 }, "id": "KMADJIBOEG_866", "locus": "GCA000292445_00865" }, { "type": "cds", "sequence": "contig_1", "start": 472332, "stop": 472916, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25", "PFAM:PF00587.30" ], "nt": "ATGGAGAATGTTTTAAAAGATTTAAATATCCAATATCAAAAAGAGATAGGAGCTGCTGCTTTTTATGGACCAAAAATTGATTTTCAGTTCAAAACAATCTTTAAAAAAATGATTACTATTGCCACCATTCAACTAGATTTTTTACTACCAGAAAAATTTGATCTAACTTATATAGATAAAAAAAATACACTAAAAAAACCAGTTATTATCCATGTTGGAATTATTGGAACTTATGAAAGGTTTATTGCTGCTTTACTTGAAAAAACAAGTGGTAATTTTCCTTTATGGTTAGCACCTGTTCAAGCCGTAATTATTCCTGTTAATATCCAAAAGCATTTAAAGGCAGCAAAAAAACTTTATAACAAATTGCTAAAAGAAAACATCCGTGTAAATTTAGATGATAATCAAGATCGCTTAGCTAAAAAAGTTAGACAAGCAATCATTGAAAAAATTCCTTTACAACTTATTGTTGGAGATAAAGAAATAGAGAATTTAGAGAAGTTGACATGCCGTGGTTTTAAAGGTGAAAAAATCACCAGAATTAGCTTTAATAATTTTGTTAAAAGAGTTAGAAGAGATGGATAG", "aa": "MENVLKDLNIQYQKEIGAAAFYGPKIDFQFKTIFKKMITIATIQLDFLLPEKFDLTYIDKKNTLKKPVIIHVGIIGTYERFIAALLEKTSGNFPLWLAPVQAVIIPVNIQKHLKAAKKLYNKLLKENIRVNLDDNQDRLAKKVRQAIIEKIPLQLIVGDKEIENLEKLTCRGFKGEKITRISFNNFVKRVRRDG", "aa_hexdigest": "2267b94af11ba60b986562197adba930", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 93, "aa_cov": 0.4690721649484536, "hmm_cov": 0.989247311827957, "evalue": 7.091065693972504e-22, "score": 72.59778594970703, "start": 101, "stop": 191 }, { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 92, "aa_cov": 0.44329896907216493, "hmm_cov": 0.9782608695652174, "evalue": 9.460731429245904e-16, "score": 53.49976348876953, "start": 3, "stop": 88 } ], "seq_stats": { "molecular_weight": 22423.315200000012, "isoelectric_point": 9.805141639709472 }, "id": "KMADJIBOEG_867", "locus": "GCA000292445_00866" }, { "type": "cds", "sequence": "contig_1", "start": 472935, "stop": 473411, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "GTGCATCACCAGCAACTATTGTTTGGTTTTAATACTGTTTGTTCTCCTGTATTAGCTAACATAGAGCTTTTTAAAATTAGCGGCCACTATCAGCACTATAAGGAAGATATGTTTCCTGCTATTAAACTTGATAGTCAAGCAATGATGCTTCGTCCTATGACATGTCCTCATCACTGTCTGATTTTCAAACAAAAACGATATTCATATAAAAAAATGCCACAGCGCTTTTCAGAAGATTCTATTTTGCATCGTTTTGAAGCCTCTGGAGGATTAATAGGATTAGAAAGAGTGAGGTGCATGACTTTACTTGATAATCACATTTTTTGTCGTGCAGATCAAATTAAAAGTGAGATTAAAAACGCATTTAATTTAATTCAAAAAGTTAATAAAAAATTTGGATTTATATTTGATAGGATAGATCTTTCTCTACATGATCCTAAAAATCAATCAAAATTTATTGATAATCCTGGTTTATGA", "aa": "MHHQQLLFGFNTVCSPVLANIELFKISGHYQHYKEDMFPAIKLDSQAMMLRPMTCPHHCLIFKQKRYSYKKMPQRFSEDSILHRFEASGGLIGLERVRCMTLLDNHIFCRADQIKSEIKNAFNLIQKVNKKFGFIFDRIDLSLHDPKNQSKFIDNPGL", "aa_hexdigest": "01c2181845851cad57c667f04accbeb6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 101, "aa_cov": 0.6329113924050633, "hmm_cov": 0.9801980198019802, "evalue": 5.1204907472873956e-18, "score": 60.883846282958984, "start": 46, "stop": 145 } ], "seq_stats": { "molecular_weight": 18530.4717, "isoelectric_point": 9.259739112854003 }, "id": "KMADJIBOEG_868", "locus": "GCA000292445_00867" }, { "type": "cds", "sequence": "contig_1", "start": 473454, "stop": 473636, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGAGATCCTTCACAATTACAACTTCAGAGGATTAATGGCATTTTTGCTCAATCAAAAAAAGAATTAGAACAATTAATAAAAGAAAATGAAGAACGCTTGAAGAAGGATCACAGATCTTTAGGTAAACAATTAGAGTTATTTAGCTTTGACCCACTGATCGGTGCAGGTCTTCCTATTTGA", "aa": "MGDPSQLQLQRINGIFAQSKKELEQLIKENEERLKKDHRSLGKQLELFSFDPLIGAGLPI", "aa_hexdigest": "2d1ceafb525218cb246457be55844758", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6846.820599999997, "isoelectric_point": 6.556002616882325 }, "id": "KMADJIBOEG_869", "locus": "GCA000292445_00868" }, { "type": "cds", "sequence": "contig_1", "start": 473709, "stop": 474026, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGCAGGAGTTCTTTTATTACAAATATGGTTAAAATGCAAATATGCTAATGTTCAGTTTGGTGAACATGGTTTTAATGGAGATGAATTTTATTTAGATTTTTATATAAATGAGAATTTTTCTACAAAACAGTTTGCAAAAATAGAATCTGATTTAAACTCTCTTTCTAGTAAACTAGAAGGGATTTCTCAAAAATTTGTTTCTTTAGATGAAGCATTAAGTTTTTTTGAAAATGATCAGTTTACAAAAAACTTATTAAAAAAAAGCAACTTAAACAAATTTAAAATCACCTTTTTTGAAAATAAACATTTTTGA", "aa": "MLAGVLLLQIWLKCKYANVQFGEHGFNGDEFYLDFYINENFSTKQFAKIESDLNSLSSKLEGISQKFVSLDEALSFFENDQFTKNLLKKSNLNKFKITFFENKHF", "aa_hexdigest": "b4b0e43b237b64ae68489a9309012765", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12294.846999999982, "isoelectric_point": 6.08395481109619 }, "id": "KMADJIBOEG_870", "locus": "GCA000292445_00869" }, { "type": "cds", "sequence": "contig_1", "start": 474098, "stop": 474247, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MG376", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75224", "UniRef:UniRef90_P47616" ], "nt": "TTGTTTGGTGAACGAAGTTTTACTGATTTTTTTGTTTTTTATGCAAATGGTCAATTAGCATTTGAACTTGAAGCATATACTCAAAAATTTAAAACAATTAGCATCGAAGGTATTCTGCGCACTTACCTAGAAAAACGCAGTGGAATTTGA", "aa": "MFGERSFTDFFVFYANGQLAFELEAYTQKFKTISIEGILRTYLEKRSGI", "aa_hexdigest": "447289fe1f00fc36f0ecc097a52e7c81", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 474026, "stop": 474340, "observations": { "mutation": [ 474100, 474295 ], "stop": [ 474100, 474295 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47616", "query_cov": 1.0, "subject_cov": 0.47115384615384615, "identity": 0.98, "score": 96.7, "evalue": 5.41e-25, "gene_start": 1, "gene_end": 49, "reference_start": 32, "reference_end": 80, "reference_sequence": "MLNCVFLEGEIESTKWSHKKTGFLVTIKQKRLFGERSFTDFFVFYANGQLAFELEAYTQKFKTISIEGILRTYLEKRSGIWKTTIEVVKIMKPNSKVMIDYQES" }, "paralog": false, "identity": 0.9807692307692307, "score": 199.0, "evalue": 4.56e-68, "description": "Internal stop codon at 474100, 474295. Nonsense mutation around 474100, 474295.", "psc": { "uniref90_id": "UniRef90_P47616", "product": "Uncharacterized protein MG376", "uniref50_id": "UniRef50_P75224", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47616", "UniRef:UniRef50_P75224" ] }, "pscc": { "uniref50_id": "UniRef50_P75224", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75224" ], "product": "Uncharacterized protein MG376 homolog" } }, "id": "KMADJIBOEG_871", "locus": "GCA000292445_00870" }, { "type": "cds", "sequence": "contig_1", "start": 474346, "stop": 474504, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTATGAAAAAACTAATCAGAATGTTAGAGAATATCTTGATGATAAACAAAAGTTTGAAGGTATTAGAACTGCATTAATAGAAGAAAGAATGGTTTTAGAAACAATTAACCACTTTAAATTTCATTTCAACTTAACAGGTCAATTACCTAGCTAA", "aa": "MYYEKTNQNVREYLDDKQKFEGIRTALIEERMVLETINHFKFHFNLTGQLPS", "aa_hexdigest": "79e6bb9022c550ad92e75000777a3f5d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6337.1151999999975, "isoelectric_point": 6.043030738830566 }, "id": "KMADJIBOEG_872", "locus": "GCA000292445_00871" }, { "type": "cds", "sequence": "contig_1", "start": 474547, "stop": 474927, "strand": "-", "frame": 1, "gene": null, "product": "MPN555 family protein chaperone", "db_xrefs": [ "GO:0006457", "GO:0015031", "SO:0001217", "UniRef:UniRef50_P75223", "UniRef:UniRef90_P47617" ], "nt": "ATGGCTACTAATCTTAAATCTATTGCAAAGTTGCAAAAACCTATTCAGTATGATAAGGTAATTGAAGTTGATCGTATTTTTGCAGATCCTGCTTTTATTGAGCAACATCGGCAAAGAATTTTAGCTTCTTTTAAAGATGCTAAGGAAAGTGCTCTTTACCACGAACTAACTCATATTGTTATTAAAGATAATTTGTTTTCAGCAGCAATGAATGAAATTGTTAGTTATTTTGAATTTCAAATTAACCCTGAAGAGTTAAAAAATGTCGTTGAAGGCTTGAAAAGAGATGTTGTTAAAGATGCTGATGAAAAAACAATTCAATCAATAGCTGAAAAAATCATTAAAAAAGCTTTAGTATTTAATTTCTTACAAAAGGAATGA", "aa": "MATNLKSIAKLQKPIQYDKVIEVDRIFADPAFIEQHRQRILASFKDAKESALYHELTHIVIKDNLFSAAMNEIVSYFEFQINPEELKNVVEGLKRDVVKDADEKTIQSIAEKIIKKALVFNFLQKE", "aa_hexdigest": "9f972991ffd1a8e89b288a8765ade07b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 474346, "stop": 474927, "observations": { "mutation": [ 474549 ], "stop": [ 474549 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47617", "query_cov": 1.0, "subject_cov": 0.6528497409326425, "identity": 1.0, "score": 241.0, "evalue": 1.69e-79, "gene_start": 1, "gene_end": 126, "reference_start": 1, "reference_end": 126, "reference_sequence": "MATNLKSIAKLQKPIQYDKVIEVDRIFADPAFIEQHRQRILASFKDAKESALYHELTHIVIKDNLFSAAMNEIVSYFEFQINPEELKNVVEGLKRDVVKDADEKTIQSIAEKIIKKALVFNFLQKEWKVEVSDDIVKRVISLYYEKTNQNVREYLDDKQKFEGIRTALIEERMVLETINHFKFHFNLTGQLPS" }, "paralog": false, "identity": 0.9948186528497409, "score": 372.0, "evalue": 6.2e-134, "description": "Internal stop codon at 474549. Nonsense mutation around 474549.", "psc": { "uniref90_id": "UniRef90_P47617", "product": "MPN555 family protein chaperone", "uniref50_id": "UniRef50_P75223", "go_ids": [ "GO:0006457", "GO:0015031" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47617", "UniRef:UniRef50_P75223", "GO:0006457", "GO:0015031" ] }, "pscc": { "uniref50_id": "UniRef50_P75223", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75223" ], "product": "Uncharacterized protein MG377 homolog" } }, "id": "KMADJIBOEG_873", "locus": "GCA000292445_00872" }, { "type": "cds", "sequence": "contig_1", "start": 474974, "stop": 475414, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05746.21" ], "nt": "ATGTTATCTCAAAATAATGGCACAATTATCAAAATTGATTTAGATATAGCTAATTTGCAAAACTCTGCTAATCCAGTTTATTATGTTCAATATGCGTTTGCAAGAATGAATAGTATTCTTAGAATTGCAAATTCTGATCAATTAAAAGAAATTACTGATTGCAGTCTTTTGATTAATGAAAAAGAGATATCACTTTTAAATCAACTTGTCTATTATCCTTTTATGTTGCAAAAAGCTATGGAAACAGGCGAATTGCACTTATTAACTAACTTTTTATATGAAACTGCTAGTTTATTTCATTCCTGGTATAAAGTTTGCAAAATTAATGATGATAAAAATTCACTTTTATCAGCACAAAGACTTGCTTTATTGAGATCATTACAATTTATAGTTAAACAAATCCTTGATGTTTTGAAGATTTCAACACCACAACAAATGTAA", "aa": "MLSQNNGTIIKIDLDIANLQNSANPVYYVQYAFARMNSILRIANSDQLKEITDCSLLINEKEISLLNQLVYYPFMLQKAMETGELHLLTNFLYETASLFHSWYKVCKINDDKNSLLSAQRLALLRSLQFIVKQILDVLKISTPQQM", "aa_hexdigest": "87c6248af5377605c2de863a68a2612b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05746.21", "name": "DALR_1", "length": 118, "aa_cov": 0.8082191780821918, "hmm_cov": 0.9915254237288136, "evalue": 1.4876811353006746e-32, "score": 107.3184814453125, "start": 29, "stop": 146 } ], "seq_stats": { "molecular_weight": 16834.410199999988, "isoelectric_point": 6.714128684997559 }, "id": "KMADJIBOEG_874", "locus": "GCA000292445_00873" }, { "type": "cds", "sequence": "contig_1", "start": 475418, "stop": 475594, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATTGTGCATTTGATGCTTTAAAAAATACCACTAAAACTTCTTTAAAAATTTTTGCATTACAACTGGTTACTCTCTATAAAAATAAAGAGCTAGTACGTTTGTCAAAACGTGCTGGAAATGTAATCACAATTGAAACAATGCTTTCAATGATTAGTGAAGATGCTGCTAGATGA", "aa": "MYCAFDALKNTTKTSLKIFALQLVTLYKNKELVRLSKRAGNVITIETMLSMISEDAAR", "aa_hexdigest": "5cf8facb3ea03ab024ad0e3e0b54929d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6554.7204, "isoelectric_point": 9.57898654937744 }, "id": "KMADJIBOEG_875", "locus": "GCA000292445_00874" }, { "type": "cds", "sequence": "contig_1", "start": 475859, "stop": 476311, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAAAAACACCTTGCTTTACTAAGAAAAATATAAAGATTTTAATTGAATCTGTATCAGCAAATCCTACCGGAAGGATCCATTTAGGTCATGTGAGAATAGCTTTTTTTGGTGATGTTTTAAACAATTTAGCCAAGCTGTTGGGTTATACAACAGTCTGTGAATATTGGGTAAATGATTATGGACAACAAGCACGAGTTTTTAGCTTTAGTGTTTATCAAAGTTTGCAGTTAAAAAAAAATATTGCTATCCAGCAACATCCTGATGGATATAGTGGAATAGTAATAGATAAAATTGCTAGTGAAATTGAAAATTTTCCAGTTGATAATTTAAATTTTGAAGAGTTTTGTAAAACATCATTCTTAGATCATTTTTTAGTTAATTGCACCCAAAAAGTTTTGTCTTTAATTAAAAGTGATTTGAATAAAATCCATGTTTTTATTGATAGTTGA", "aa": "MIKTPCFTKKNIKILIESVSANPTGRIHLGHVRIAFFGDVLNNLAKLLGYTTVCEYWVNDYGQQARVFSFSVYQSLQLKKNIAIQQHPDGYSGIVIDKIASEIENFPVDNLNFEEFCKTSFLDHFLVNCTQKVLSLIKSDLNKIHVFIDS", "aa_hexdigest": "7816ca1076a1f28b8e3604d69c9ac6ab", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17073.515300000006, "isoelectric_point": 7.690679359436037 }, "id": "KMADJIBOEG_876", "locus": "GCA000292445_00875" }, { "type": "cds", "sequence": "contig_1", "start": 476408, "stop": 476587, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTTATCATCAATGATTTAAAAGAATGCATTAGCGCTTTAAAGCTTAAATTTGATGATCAAAAGGAACTTGTTAAACTAGTTAAAAATAATAGTTTTAATGGTTTTTCTTCAACTATTATTTTCCAACTAAAAAGTGAAAATCATAAAAAAATTGCAGATAGTATTGTTGAGTGA", "aa": "MFFIINDLKECISALKLKFDDQKELVKLVKNNSFNGFSSTIIFQLKSENHKKIADSIVE", "aa_hexdigest": "ac98275191574655d733bb35ae25a931", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6820.880599999999, "isoelectric_point": 7.868640327453613 }, "id": "KMADJIBOEG_877", "locus": "GCA000292445_00876" }, { "type": "cds", "sequence": "contig_1", "start": 476616, "stop": 477920, "strand": "+", "frame": 3, "gene": "mnmG", "product": "tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A654IKD5" ], "nt": "ATGTCTTTTATCATAACAGTAATAGGTGCTGGGCATGCTGGATTGGAAGCCGCTTTCATTGTAAGCAAATTCAACATCAAAGTAAACCTTTTAGTTCTTGATATAAATCATTTAGGTTCTTGTCCATGTAATCCTTCAATTGGTGGACCTGCTAAGGGAATTGTTACTAGGGAAATTGATGTTTTAGGAGGTATGCAAGCAATTGCTGCTGATAACAATGCCTTACAATATAAATTACTAAATAGTTCAAAAGGACCTGCTGTGCAAGCTATCAGAGCACAAATTGACAAAATAGGTTATAAAAACTGGTTTCAAAGTCAAGTTAAATTAAATAAAAACATTAATCTAATTCAATCTGAAGCAATCAATTTAATTGTTAGAAATGAAAAAATAAAAGGCGTTATTTTAAAAGACGGAAGTGAACTTTTAAGTGATGCGGTTATTATCACTACCGGAACGTACCTAAGATCAAAAACATACTGTGGTAATACAGTTAAAAATCAAGGACCTGATCAATCTAAAAATAGTGAAAAATTAAGCACAAACTTAATTAACAGAGGTTTTAAAACAATTCGTTTAAAAACAGGAACTCCGCCAAGAATTTTAAAAACTTCACTTGACTATAATCAAATGGAATTAGAAATTAATAATAATCAAAACCTTGCTTTTAGTACTACAAATAAAAATTTCTTACCACTTGAAAAACAAATACCTTGTTACTTAGTTCATACCAATCAAAAAATTCACGATCTAATCCTTAAAAACTTAAAAAAATCTGCAATGTTTAATGGTAGTATTTCAGCACAAGGACCACTTTATTGTCCAAGCATTGAAGACAAAGTTTTTAAGTTCTCTCAAAAACCTCGTCACCAAATTTTTGTAGAACCTGAATCATTGAGTCTAGATACTATTTATTTAGCAGGATTATCAACTTCTTTTACACCAGAAATTCAAAAAGAAATCATCCAGCTTTTACCTGGTTTTCAAAATGCAAAAATTAAAAAGTTTGGTTACGCTATTGAATATGATGCTTTTCTATCTAATCAACTAAAACCAACACTTGAAACGAAGTTAATAGAAAACTTGTATTTTGCTGGACAAATTAATGGCACTAGCGGTTATGAAGAAGCTGCTGGTCAAGGTTTGATGGCAGGAATTAATGCTGCTTTAAAATTATTAAAAAAACCACCATTTATTTTGCAACGTAATGAGGCTTATATTGGGGTTATGATTAATGATTTAGTTACTAAAACAATCAGTGATCCATACCGTTTGTTAACATCCAGAGCAGAATATAGACTATGA", "aa": "MSFIITVIGAGHAGLEAAFIVSKFNIKVNLLVLDINHLGSCPCNPSIGGPAKGIVTREIDVLGGMQAIAADNNALQYKLLNSSKGPAVQAIRAQIDKIGYKNWFQSQVKLNKNINLIQSEAINLIVRNEKIKGVILKDGSELLSDAVIITTGTYLRSKTYCGNTVKNQGPDQSKNSEKLSTNLINRGFKTIRLKTGTPPRILKTSLDYNQMELEINNNQNLAFSTTNKNFLPLEKQIPCYLVHTNQKIHDLILKNLKKSAMFNGSISAQGPLYCPSIEDKVFKFSQKPRHQIFVEPESLSLDTIYLAGLSTSFTPEIQKEIIQLLPGFQNAKIKKFGYAIEYDAFLSNQLKPTLETKLIENLYFAGQINGTSGYEEAAGQGLMAGINAALKLLKKPPFILQRNEAYIGVMINDLVTKTISDPYRLLTSRAEYRL", "aa_hexdigest": "ecb5d7bc4d09619d897b4352fdf80dc5", "start_type": "ATG", "rbs_motif": "TTATA", "psc": { "uniref90_id": "UniRef90_UPI0030D362B2", "query_cov": 0.9423963133640553, "subject_cov": 0.9975609756097561, "identity": 0.531, "score": 434.0, "evalue": 1.37e-147, "valid": false, "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "uniref50_id": "UniRef50_A0A654IKD5", "kegg_orthology_id": "K03495", "go_ids": [ "GO:0002098", "GO:0008033" ] }, "pscc": { "uniref50_id": "UniRef50_A0A654IKD5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A654IKD5" ], "product": "tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG" }, "genes": [ "mnmG" ], "id": "KMADJIBOEG_878", "locus": "GCA000292445_00877" }, { "type": "cds", "sequence": "contig_1", "start": 478076, "stop": 478453, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF21680.2", "PFAM:PF13932.14" ], "nt": "TTGAAATTCACTAATAAAAATACCGCTCAATCACTTTATGACTTCAACAAACGAAGTGAAATAAATTTAGATAAATTGATCAAAGATCTTCCTGAAAAATACCAATTAGATTCAGAAACACTTAAACAAATTGAAATTGAAATTAAATATGAGGGTTACATAAAGAAAAATGAAAAGTATTTTAAGGGTTTAGATAAATTAAGCAAAATTAAAATTCCTCATACTTTTGATTACCATAAGGTTAAGAATTTAGCTAGTGAAGCTATTTTTAAACTATCTAACTTTAAGCCTAGTAATTTAGCAATTGCAAGTCAAATAGCTGGAGTGAACTTTAATGACATTATAGCCATAAAACATTTTTTAAAAACTTATGAATAA", "aa": "MKFTNKNTAQSLYDFNKRSEINLDKLIKDLPEKYQLDSETLKQIEIEIKYEGYIKKNEKYFKGLDKLSKIKIPHTFDYHKVKNLASEAIFKLSNFKPSNLAIASQIAGVNFNDIIAIKHFLKTYE", "aa_hexdigest": "7ae88dffae1ecfa14723687d25439059", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF21680.2", "name": "GIDA_C_1st", "length": 52, "aa_cov": 0.416, "hmm_cov": 1.0, "evalue": 4.064407719709777e-14, "score": 48.32313537597656, "start": 6, "stop": 57 }, { "id": "PF13932.14", "name": "SAM_GIDA_C", "length": 53, "aa_cov": 0.424, "hmm_cov": 1.0, "evalue": 1.3278625562877846e-16, "score": 55.32765579223633, "start": 65, "stop": 117 } ], "seq_stats": { "molecular_weight": 14651.758899999975, "isoelectric_point": 9.337681388854982 }, "id": "KMADJIBOEG_879", "locus": "GCA000292445_00878" }, { "type": "cds", "sequence": "contig_1", "start": 478446, "stop": 479024, "strand": "+", "frame": 3, "gene": "rsmG", "product": "16S rRNA (guanine(527)-N(7))-methyltransferase RsmG", "db_xrefs": [ "COG:COG0357", "COG:J", "EC:2.1.1.-", "EC:2.1.1.170", "GO:0005829", "GO:0008168", "GO:0031167", "GO:0046118", "GO:0070043", "KEGG:K03501", "RefSeq:WP_009885942.1", "SO:0001217", "UniParc:UPI000012B473", "UniRef:UniRef100_P47620", "UniRef:UniRef50_P75220", "UniRef:UniRef90_P47620" ], "nt": "ATGAATAATGCTAATTTTGAAAAATATGTTGATTTAGTTTTTGAAGCAAACAAAAATTTCAACTTAACAGGATTTAAAACAAAAGAAGCTATTTATCAGAATTTAGTTATAGAAATATTGACATTATTTAAAGGATATGAAAAATTTTTTATTGACAAAACTGTAGCAGACTTGGGAAGTGGAAATGGTTCGCCTGGGATAATATTAAAACTGTTATTTCAAAAAATAAAAAAGTTAGTTTTAATTGATAGTAAACACAAAAAAATTAGCTTTTTAAATAAATTAACTAAGCAACTAAATCTGGAGAAAACTGTTGCAATTTGTGAACGAATTGAAGTACATAAAAATCACTATGATGTTATCTGTTCTCGTGGTCTAAGTACGATTATTAAAGTTAATGATTTAGCATTTTCCTTGCTTAACTCAAAAGGTATTATTTTTCATATAAAACAAAGCTTAGACCAATACATTGAATTTGAAAAATCAAATCAGAAGAATCAATTTAACTTGTTATTTATAAAGCACTTTACTAGTCAGAATAAAAAACTAATTTTGATAGCTTTACAAAAAAATGATTAA", "aa": "MNNANFEKYVDLVFEANKNFNLTGFKTKEAIYQNLVIEILTLFKGYEKFFIDKTVADLGSGNGSPGIILKLLFQKIKKLVLIDSKHKKISFLNKLTKQLNLEKTVAICERIEVHKNHYDVICSRGLSTIIKVNDLAFSLLNSKGIIFHIKQSLDQYIEFEKSNQKNQFNLLFIKHFTSQNKKLILIALQKND", "aa_hexdigest": "a4edbded6dab27c0c17cc18c7eaea59b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012B473", "ncbi_nrp_id": "WP_009885942.1", "uniref100_id": "UniRef100_P47620", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012B473", "RefSeq:WP_009885942.1", "UniRef:UniRef100_P47620" ] }, "ips": { "uniref100_id": "UniRef100_P47620", "uniref90_id": "UniRef90_P47620" }, "psc": { "uniref90_id": "UniRef90_P47620", "gene": "rsmG", "product": "16S rRNA (guanine(527)-N(7))-methyltransferase RsmG", "ec_ids": [ "2.1.1.-", "2.1.1.170" ], "uniref50_id": "UniRef50_P75220", "cog_id": "COG0357", "cog_category": "J", "kegg_orthology_id": "K03501", "go_ids": [ "GO:0005829", "GO:0008168", "GO:0031167", "GO:0046118", "GO:0070043" ] }, "pscc": { "uniref50_id": "UniRef50_P75220", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75220" ], "product": "Ribosomal RNA small subunit methyltransferase G" }, "genes": [ "rsmG" ], "id": "KMADJIBOEG_880", "locus": "GCA000292445_00879" }, { "type": "cds", "sequence": "contig_1", "start": 479329, "stop": 479673, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MG381", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75219", "UniRef:UniRef90_P47621" ], "nt": "ATGTTGATTGATGTTCAACATCATAAAAAGTTACAAAAATGTTTATTAAGAGAAATTCATCTTTTAGTTTGTTTAATTAATAGCAATCAATTTAGTGATGAATTAATGCAAATTATTGAGTGGTATCAGTATTTAAAAAAACACTCTAGTAAACTTTTTGTTATTACTGCAAGTAGTGATAAAAAACCAGTAATAGAGCCAACTATAAATGAATATAAAGCTATTTTTGGAGAGTATTTATCTTCATTTCACCTAGATCTAAAAAATAATCAAAGTAATGATTTATTCCAGAAATTACTTGATCAGATCAAAATAAAAGCTACCTCAAAAACTAGTTTGAGATAG", "aa": "MLIDVQHHKKLQKCLLREIHLLVCLINSNQFSDELMQIIEWYQYLKKHSSKLFVITASSDKKPVIEPTINEYKAIFGEYLSSFHLDLKNNQSNDLFQKLLDQIKIKATSKTSLR", "aa_hexdigest": "deb57aafa407fa60704d744034546606", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 479029, "stop": 479673, "observations": { "mutation": [ 479032 ], "stop": [ 479032 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47621", "query_cov": 1.0, "subject_cov": 0.5229357798165137, "identity": 1.0, "score": 224.0, "evalue": 8.23e-73, "gene_start": 1, "gene_end": 114, "reference_start": 105, "reference_end": 218, "reference_sequence": "MIKLGWASFSNTEFAQDIFIKFANQFYKQDDAGTILFELKKTLGVNQLDEIEKNKKVIIVNQFQNSLGKFLLFNKENTKRINSLANEHISSFLKKIFRLSGFKDMLIDVQHHKKLQKCLLREIHLLVCLINSNQFSDELMQIIEWYQYLKKHSSKLFVITASSDKKPVIEPTINEYKAIFGEYLSSFHLDLKNNQSNDLFQKLLDQIKIKATSKTSLR" }, "paralog": false, "identity": 0.9953271028037384, "score": 419.0, "evalue": 3.69e-152, "description": "Internal stop codon at 479032. Nonsense mutation around 479032.", "psc": { "uniref90_id": "UniRef90_P47621", "product": "Uncharacterized protein MG381", "uniref50_id": "UniRef50_P75219", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47621", "UniRef:UniRef50_P75219" ] }, "pscc": { "uniref50_id": "UniRef50_P75219", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75219" ], "product": "Uncharacterized protein MG381 homolog" } }, "id": "KMADJIBOEG_881", "locus": "GCA000292445_00880" }, { "type": "tRNA", "sequence": "contig_1", "start": 479687, "stop": 479762, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(tct)", "amino_acid": "Arg", "anti_codon": "tct", "score": 73.5, "nt": "GCGTCGGTAGCTCAGTGGATAGAGTACACGCCTTCTAAGCGTGTTGTCGTGGGTTCAAATCCCTCCCGATGCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 479727, 479729 ], "id": "KMADJIBOEG_17", "locus": "GCA000292445_00881" }, { "type": "cds", "sequence": "contig_1", "start": 480138, "stop": 480236, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGAAAAAGGGATTTTAGTTGCAATTAGTGGTGGTAGTTGCTCAGGAAAAACTACTGTTGCTGAAATGATTTATCAACTTTTAAGTAAAAATTAA", "aa": "MDEKGILVAISGGSCSGKTTVAEMIYQLLSKN", "aa_hexdigest": "61fce44a15ee4cd2b66ecc9d8dbeaebc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3344.8731000000007, "isoelectric_point": 5.934638786315918 }, "id": "KMADJIBOEG_882", "locus": "GCA000292445_00882" }, { "type": "cds", "sequence": "contig_1", "start": 480775, "stop": 480930, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCATTACTTATAAGGAACTTGATCAATATTTAAAAGGTGATTTAATACTTAGTTCAGAAAAGCAAAAAATTGTTTTAGATTTGAAAGCAAAAGCAGAGCATAAACATAATTCACCTTTGAAATTTAAACATCTCTATAATTTCCAGAACTAA", "aa": "MGITYKELDQYLKGDLILSSEKQKIVLDLKAKAEHKHNSPLKFKHLYNFQN", "aa_hexdigest": "9fda724ba694131d3d3ba14dab2e308e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6016.9207, "isoelectric_point": 9.302675056457517 }, "id": "KMADJIBOEG_883", "locus": "GCA000292445_00883" }, { "type": "cds", "sequence": "contig_1", "start": 481078, "stop": 481521, "strand": "-", "frame": 1, "gene": "nadE", "product": "NH(3)-dependent NAD(+) synthetase", "db_xrefs": [ "COG:COG0171", "COG:H", "EC:6.3.1.5", "GO:0003952", "GO:0004359", "GO:0005524", "GO:0005737", "GO:0008795", "GO:0009435", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47623", "UniRef:UniRef90_P47623" ], "nt": "ATGACTAATTTAATTAAATATTTAAAAGAACTCCAAAACTGGCTGTTTGATTATGTAAAAAAATCTAAAGCTAAAGGTGTTATTTTTGGCTTATCTGGAGGAATTGATTCAGCAGTTGTTGCTGCTATTGCTAAAGAAACTTTTGGTTTTGAAAACCATTTAGCTTTAATAATGCATATTAATAATTCAAAACTTGATTTTCAAGCAACTAGTGAACTTGTTAAAAAAATGCAATTTAATAGTATTAACATTGAACTGGAAGAGAGTTTCAATCTGTTAGTAAAAACCCTTGGAATAGATCCAAAAAAAGATTTTTTAACAGCTGGTAACATTAAAGCACGTTTACGGATGATAACTTTATATGCTTATGCTCAAAAACACAACTTCTTAGTTTTAGGTACTGGTAATTTTGTAGAGTATACACTTGGTTATTTCACAAAATGA", "aa": "MTNLIKYLKELQNWLFDYVKKSKAKGVIFGLSGGIDSAVVAAIAKETFGFENHLALIMHINNSKLDFQATSELVKKMQFNSINIELEESFNLLVKTLGIDPKKDFLTAGNIKARLRMITLYAYAQKHNFLVLGTGNFVEYTLGYFTK", "aa_hexdigest": "b32fde3d78a1939af3058617b114fb9f", "start_type": "ATG", "rbs_motif": null, "genes": [ "nadE" ], "truncated": "5-prime", "pseudogene": { "start": 480775, "stop": 481521, "observations": { "mutation": [ 481080 ], "stop": [ 481080 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47623", "query_cov": 1.0, "subject_cov": 0.592741935483871, "identity": 1.0, "score": 290.0, "evalue": 8.23e-98, "gene_start": 1, "gene_end": 147, "reference_start": 1, "reference_end": 147, "reference_sequence": "MTNLIKYLKELQNWLFDYVKKSKAKGVIFGLSGGIDSAVVAAIAKETFGFENHLALIMHINNSKLDFQATSELVKKMQFNSINIELEESFNLLVKTLGIDPKKDFLTAGNIKARLRMITLYAYAQKHNFLVLGTGNFVEYTLGYFTKWGDGACDIAPLAWLLKEDVYKLAKHFNIPEIVITRAPTASLFEGQTDETEMGITYKELDQYLKGDLILSSEKQKIVLDLKAKAEHKHNSPLKFKHLYNFQN" }, "paralog": false, "identity": 0.9959677419354839, "score": 491.0, "evalue": 1.31e-179, "description": "Internal stop codon at 481080. Nonsense mutation around 481080.", "psc": { "uniref90_id": "UniRef90_P47623", "gene": "nadE", "product": "NH(3)-dependent NAD(+) synthetase", "ec_ids": [ "6.3.1.5" ], "uniref50_id": "UniRef50_P47623", "cog_id": "COG0171", "cog_category": "H", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0004359", "GO:0046872", "GO:0003952", "GO:0008795", "GO:0009435" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47623", "EC:6.3.1.5", "UniRef:UniRef50_P47623", "COG:COG0171", "COG:H", "GO:0005737", "GO:0005524", "GO:0004359", "GO:0046872", "GO:0003952", "GO:0008795", "GO:0009435" ] }, "pscc": { "uniref50_id": "UniRef50_P47623", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47623" ], "product": "NH(3)-dependent NAD(+) synthetase" } }, "id": "KMADJIBOEG_884", "locus": "GCA000292445_00884" }, { "type": "cds", "sequence": "contig_1", "start": 481697, "stop": 482677, "strand": "-", "frame": 3, "gene": null, "product": "GTPase Obg", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q2S3C0" ], "nt": "TTGCAAGCTGATCATAACTGCGATTCACTTTTTTTCTTGAAGAATAAAAAACACCTGTTTGCAGAAGATGGACAAAATGGTAAACCAGATTTAGCACATGGTAAAAATGGTTCAGATCTTTTAATTAAAGTGCCAATAGGAACAACAGTTAAAAACTTAGAAAATAATAGTGTATTGGTGGATTTTGTTCATGATAAACAGAGTTTTATTTTGTGTTTTGGCGGAAAAGGTGGAAAAGGAAATGCTGCTTTTAAAAGCCCAATTATGCGTGCTCCTAATCTATATGAAAATGGTGATAAAGGAGAGATTTTAAACGTTAGTTTGGAAGTTAAATATTTAGCTAATGTAGGAATTGTTGGTTTTCCAAATTCAGGTAAATCAACACTAATCTCCAAACTTTCAAATGCCAAACCTAAAATTGCAAACTATCGCTTTACTACTTTAATACCCGTACTTGGTGTAGTTAAATATCAAAATAATAGTTTAGTTTTTGCTGATATTCCTGGTTTGATTGAAAATGCTAGTGAAGGAAGTGGGTTAGGACATGATTTTTTACGGCATATAGAGAGGTGTGAAATATTAATTCATTTAATTTCACTTGATCCTGTAGATAATGATGATCCTTGCAAAGCATATTTACAAATAATGGATGAACTTTCAAAATATTCTCCCCTTCTAGTTAAAAAAAAGATGCTTGTTGTAGCTAATAAAATTGATGTTAATGAAGGTGAGAAACGGTTTAAAAAATTAGAAAAATTTCTTCAAAAAAAATCTATTTCTGTATTAAAAATTTCTGCTTTAAAGAAAGAATTAGGCAATTTACTTGATAGAGTTTTTGAACTTTACAATAAAACAATTTCCCAATTTGGAGCTAATAAGTTTAGTTTGCCAATGGAACTTGAGAAGCATTATGTTTTTCAAAATACCAATGAAAATAATAACGACCCTTTAAATATTGAAAAAGATAGTTTAAACAGATGA", "aa": "MQADHNCDSLFFLKNKKHLFAEDGQNGKPDLAHGKNGSDLLIKVPIGTTVKNLENNSVLVDFVHDKQSFILCFGGKGGKGNAAFKSPIMRAPNLYENGDKGEILNVSLEVKYLANVGIVGFPNSGKSTLISKLSNAKPKIANYRFTTLIPVLGVVKYQNNSLVFADIPGLIENASEGSGLGHDFLRHIERCEILIHLISLDPVDNDDPCKAYLQIMDELSKYSPLLVKKKMLVVANKIDVNEGEKRFKKLEKFLQKKSISVLKISALKKELGNLLDRVFELYNKTISQFGANKFSLPMELEKHYVFQNTNENNNDPLNIEKDSLNR", "aa_hexdigest": "7b8985391b5fb4e4d156ae57c3e0421d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A432RYP9", "query_cov": 0.8773006134969326, "subject_cov": 0.8461538461538461, "identity": 0.5, "score": 274.0, "evalue": 3.27e-87, "valid": false, "gene": "obgE", "product": "GTPase ObgE", "uniref50_id": "UniRef50_Q2S3C0", "go_ids": [ "GO:0003924" ] }, "pscc": { "uniref50_id": "UniRef50_Q2S3C0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q2S3C0" ], "product": "GTPase Obg" }, "genes": [], "id": "KMADJIBOEG_885", "locus": "GCA000292445_00885" }, { "type": "cds", "sequence": "contig_1", "start": 482815, "stop": 483060, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTATCTTACTTCGATTTTTTTTAGTTCTAATAACATTAATGCTAAGTTTTTTCTTTTGAATAAAATGACTAACTTTTTTTTAATCAGATCAAAATATGCAATAATAATTGCTGAAAATCTCTTAAAGACAGGTAAATCTAAAACTAAAAATCGACAAAAGTTGCAACAAATTCAAAGTGATCTAGAAAGAAAAAAATCTGCAGAAATCGTTTTTAAAATAAAGAAAAAGAAACCAAAATAA", "aa": "MNYLTSIFFSSNNINAKFFLLNKMTNFFLIRSKYAIIIAENLLKTGKSKTKNRQKLQQIQSDLERKKSAEIVFKIKKKKPK", "aa_hexdigest": "daa862860eae3f32641fbde1795c7967", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9559.296199999993, "isoelectric_point": 10.530475425720216 }, "id": "KMADJIBOEG_886", "locus": "GCA000292445_00886" }, { "type": "cds", "sequence": "contig_1", "start": 483515, "stop": 483961, "strand": "-", "frame": 3, "gene": "ugpQ", "product": "putative protein MG385", "db_xrefs": [ "COG:COG0584", "COG:I", "GO:0006629", "GO:0008081", "SO:0001217", "UniRef:UniRef50_P47625", "UniRef:UniRef90_P47625" ], "nt": "TTGAATTTAAACCAAATTAATTTAGTTGATCTTAAAAAGGTTAACCTTGCAAGTGAATTTAAGCTTAAGGTAACTTCACAACAAATTCAAACTTTAAAGGAAGTTTTAACGCAATTTATTCAGCCCTTTAAATATTTGCTGATTCATATTAAAGATGATAAAGAAAATAACAATTCATTATTAGAACAGTTGAATTTGCTTTGCAAAGATTTTGTCTTAGCTAAAGAAAAAATGATTTTGTTAAGTAGTAATTTTCATATCATTAAGTTAATAAATGAGACTTTCAAAGGCTTTAAAACCGGTTTTGTAATTGCAAACAAAAAAGCTTTATTAGTAGCTTCTAGAGACGAATTAATGAGATATTGCCGTTTTTTAGTGCCAAACGAGAGTTTTTATCATAAGAACTGCAAAGAAATTCAAAACCTGGGATTACCAGTTATTCTTTGA", "aa": "MNLNQINLVDLKKVNLASEFKLKVTSQQIQTLKEVLTQFIQPFKYLLIHIKDDKENNNSLLEQLNLLCKDFVLAKEKMILLSSNFHIIKLINETFKGFKTGFVIANKKALLVASRDELMRYCRFLVPNESFYHKNCKEIQNLGLPVIL", "aa_hexdigest": "5e07803e801b973a2b11c61d93e0224b", "start_type": "TTG", "rbs_motif": null, "genes": [ "ugpQ" ], "truncated": "both", "pseudogene": { "start": 483434, "stop": 484144, "observations": { "mutation": [ 483517, 484039 ], "stop": [ 483517, 484039 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47625", "query_cov": 1.0, "subject_cov": 0.6271186440677966, "identity": 0.993, "score": 287.0, "evalue": 9.43e-97, "gene_start": 1, "gene_end": 148, "reference_start": 62, "reference_end": 209, "reference_sequence": "MRKQFLIAYRGYSSIAPENTKLAFQAAQVFDFDGIWIDIQLTKDKQIVVTHKENFKVSNKNLNLNQINLVDLKKVNLASEFKLKVTSQQIQTLKEVLTQFIQPFKYLLIHIKDDKENNNSLLEQLNLLCKDFVLAKEKMILLSSNFHIIKLINETFKGFKTGFVIANKKALLVASRDELMRYCRFLVPNESFYHKNCKEIQNLGLPVILWVIKGLLRYQFYENDRFVKFQITAQIY" }, "paralog": false, "identity": 0.9872881355932204, "score": 455.0, "evalue": 1.27e-165, "description": "Internal stop codon at 483517, 484039. Nonsense mutation around 483517, 484039.", "psc": { "uniref90_id": "UniRef90_P47625", "gene": "UgpQ", "product": "Uncharacterized protein MG385", "uniref50_id": "UniRef50_P47625", "cog_id": "COG0584", "cog_category": "I", "go_ids": [ "GO:0008081", "GO:0006629" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47625", "UniRef:UniRef50_P47625", "COG:COG0584", "COG:I", "GO:0008081", "GO:0006629" ] }, "pscc": { "uniref50_id": "UniRef50_P47625", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47625" ], "product": "Uncharacterized protein MG385" } }, "id": "KMADJIBOEG_887", "locus": "GCA000292445_00887" }, { "type": "cds", "sequence": "contig_1", "start": 484037, "stop": 484144, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGAAAACAGTTTTTAATAGCTTATCGCGGTTATAGTTCTATTGCACCAGAAAACACCAAATTAGCATTTCAAGCAGCCCAAGTTTTTGATTTTGATGGTATTTGA", "aa": "MRKQFLIAYRGYSSIAPENTKLAFQAAQVFDFDGI", "aa_hexdigest": "096cf6b28b10c2b2b4fc0ba311cdc327", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3997.532699999999, "isoelectric_point": 8.188854789733888 }, "id": "KMADJIBOEG_888", "locus": "GCA000292445_00888" }, { "type": "cds", "sequence": "contig_1", "start": 484392, "stop": 484661, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTCATTATGAACCTGATGGAGAATGAGTTTGAGCAGGATTCTTTGGTGATGATCAAAAATGAAATAAGGATGCAACTGTTAAATGAGCTAGAGAAAGAGATTATTTACCACTTATTGGTGATGAAGTTTATGGTAGGTACAACAATAAAGGTGAGTGGATTTGATATGGTTTCTATGATGAATCTGGAGACTGAGTTCTTGTTGATGAACAATGAAAGAATCGTCAACCACGAATTAACGAGGCACCTAAGTTTTGAGAGAAATTAA", "aa": "MVIMNLMENEFEQDSLVMIKNEIRMQLLNELEKEIIYHLLVMKFMVGTTIKVSGFDMVSMMNLETEFLLMNNERIVNHELTRHLSFERN", "aa_hexdigest": "8707d5869708f37541a4b5a16d0e55d5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10682.52679999999, "isoelectric_point": 4.9306348800659165 }, "id": "KMADJIBOEG_889", "locus": "GCA000292445_00889" }, { "type": "cds", "sequence": "contig_1", "start": 484687, "stop": 487092, "strand": "-", "frame": 1, "gene": null, "product": "Protein P200", "db_xrefs": [ "GO:0020035", "SO:0001217", "UniRef:UniRef50_Q49429", "UniRef:UniRef90_Q49429" ], "nt": "TTGGAATATAAAGTAGATTTCCTTGAAACTGAACCAAAATCATTATTTGATGAAAAAACAACAATAGTTGTAGAAAGTGAACCACCTTTCATTCAACCTGACCTATCACTTGAATTAGATTCTGTAAATGATGTTGATAAATCATTAGAAACTAAAACTACATCTGTTGAATTAAATCATGAAGAAATTGGTAATGAATTTATTAATTTAGATGTTTCTGAAAAAGAAGTTCAAGAACAACCAACAACACAACTAGAAACTGATAGTGAGTTCGTATTACCAACATATCAAATAGTTGAAGACAGTTTCACTGAAAGTGCTGAAACTCCTAATGAATTTTCATCTGAACAAAAAGATACGCTTGAATTCATTAGTCAAACTCAAGAGGTTGAGACTTCTGAATCAAATGTACCCACAGTTGAACAAGAAACAAAATTATTTGAACATCAAGATGAAAATAACTTATTCACTCCTCTACCGCTTGATTTAACTGAAATCATTGAATCAAATGCTTTATTTGATTCAAAACCAGACGAAAAAGAGAGTTCTGAATCAGAACTCCAACCTACTTTCAAAGAAATTAAATTAGATTCAACTGTTGAAGTTCCTCAAGAATCATCACAAGTTGAAGCTACTTTTGATACTGTTCAACCAGAAGCAGTTTTTGATGAAATTAAAACTCAAGAACTACAGCCTGAAGCTACTACTGAAGTTGTTTTTGATGATCATTTTCAACCAGATGTTCAACCTGAACAAACTCCTCAAGAAGCTAAATTTGATTCACCAGTCGAAATTCCCCAAGAATCATCGCAAGCTGAATTTCATGCTGAACAAATCTCTGATGAAATTAAATTAGAAGAAAAAACTGAAGCAGTTTTTGATCATCAACAATTAGAAAATCAGTCTGAAGAAACAGTTGTTACACCTACTGAAGTTACTGCTTTTGAACCAGAAACAATTGAAACCCAATTAGAACCTAGCTCTGAAGATCAACCATCTGAACCTGCATTAGATCAAAATCATCCAGAAATTGTTACTGCTGAAGTTGAACAAATCTTTGATGGAACCAAGTTAGAAGATTTAAAACTAGAGGAAGCTAGCTTTGATAATGTTGAAAATAATGAAGTTCAACCTAAAGAAACAGAAGCTGAAATTACTTTTGATGAAACTAAAGAACTTCAACAGGAAACCTCCTCTGAACCATTATCAACAGAAGAATTAAAATCTGAAGCTACTTTTGATAATGTATCTGAAGCTGAATCAGAAGCAGTTTTTGAAAAACCTCAATTAGAAACACAGACTGAAAAAATATTAGAAGAGGAACCTAAATCTGAACCAGTTGATCAATTAATAACTGAAGCTAGCTTTGATACTGTTAAACATGAAGCAGTTTTTGATAAAAATCAAACTCAAACTGAGGGATTGGAAGAACCTCAAGTTTCTAGTGAAGCTGAAGTAGTTGATCAAACAACTACTGATACTGTTGGAGAACCTGAAGCAGTTTTTGATGTTCAACCTGAAAAAACTACTGAAGTTAAGTTTGATGATGTTGAAAATCAACAAAAAGTTATTTCTGAACCTCAAGTAGAACAACAACCTGGAGAAGCAGTTTTTGAACCATCTGCAGAAGCTAAATTTGATTCACCAGTTGAATCGGTTCAAGATTCACAACCTGAACCTGTCCTTGAAGAAGTTCAAACTCAACCAGAAATTCAACCTGTAGAATCTCAACCAGAAGCTACCTTTGATACTGTTCAACCTGAACAAACTCCTCAAGAAGCTAAATTTGATTCACCAGTTGAAACTGTTGAACAACCTGAATTTAGTAGTGAACCAACTCAACAACATGTTGAATCAGAAGCTAGTTTTGATGAACCTAATTATGACTTTGATGAACCTAACTATGATTTTGATCAACCTAGTTATGATTCAGATCTACAACCATCAGAACCTCAATATGATGTAGATGAACCTAATTATGACTTTGATGAACCAAATTATGAAATTGAATCAAAACCATCAGAGCCACAATTTGAACCTCAAGTAGAACAACAACCTGGAGAAGCAGTTTTTGAACCATCTGCAGAAGCTAAATTTGATTCACCAGTTGAATCGGTTCAAGATTCACAACCTGAACCTCTCCTTGAAGAAGTTCAAACTCAACCAGAAATTCAACCTGTAGAATCTCAACCAGAAGCTACTTTTGATACTGTTCAACCTGAACAAACTCCTCAAGAAGCTAAATTTGATTCACCAGTTGAAACTATTCAAGAACCTCAAGTTTCTAGTGAACCTGAAGTAGTTGTTCAACCTAACTTTGAAGAACGAAAACCTGAAACAGTATTGGAAGAACCTCAAGCTGATGAAATTCAACCAGAAGCTAGTGAAGAAGAATCACTTGATTGA", "aa": "MEYKVDFLETEPKSLFDEKTTIVVESEPPFIQPDLSLELDSVNDVDKSLETKTTSVELNHEEIGNEFINLDVSEKEVQEQPTTQLETDSEFVLPTYQIVEDSFTESAETPNEFSSEQKDTLEFISQTQEVETSESNVPTVEQETKLFEHQDENNLFTPLPLDLTEIIESNALFDSKPDEKESSESELQPTFKEIKLDSTVEVPQESSQVEATFDTVQPEAVFDEIKTQELQPEATTEVVFDDHFQPDVQPEQTPQEAKFDSPVEIPQESSQAEFHAEQISDEIKLEEKTEAVFDHQQLENQSEETVVTPTEVTAFEPETIETQLEPSSEDQPSEPALDQNHPEIVTAEVEQIFDGTKLEDLKLEEASFDNVENNEVQPKETEAEITFDETKELQQETSSEPLSTEELKSEATFDNVSEAESEAVFEKPQLETQTEKILEEEPKSEPVDQLITEASFDTVKHEAVFDKNQTQTEGLEEPQVSSEAEVVDQTTTDTVGEPEAVFDVQPEKTTEVKFDDVENQQKVISEPQVEQQPGEAVFEPSAEAKFDSPVESVQDSQPEPVLEEVQTQPEIQPVESQPEATFDTVQPEQTPQEAKFDSPVETVEQPEFSSEPTQQHVESEASFDEPNYDFDEPNYDFDQPSYDSDLQPSEPQYDVDEPNYDFDEPNYEIESKPSEPQFEPQVEQQPGEAVFEPSAEAKFDSPVESVQDSQPEPLLEEVQTQPEIQPVESQPEATFDTVQPEQTPQEAKFDSPVETIQEPQVSSEPEVVVQPNFEERKPETVLEEPQADEIQPEASEEESLD", "aa_hexdigest": "8d84da3315e77df9bc6acab80af47af5", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 484384, "stop": 487392, "observations": { "mutation": [ 484404, 484446, 484467, 484575, 484599, 484629, 484635, 484689 ], "stop": [ 484404, 484446, 484467, 484575, 484599, 484629, 484635, 484689 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49429", "query_cov": 1.0, "subject_cov": 0.49566831683168316, "identity": 0.9940000000000001, "score": 1045.0, "evalue": 0.0, "gene_start": 1, "gene_end": 801, "reference_start": 636, "reference_end": 1436, "reference_sequence": "MPKTTKNKNKNTTPKSKTKKYLESANKKSVTKPKKEQDKVENLFDQPFLGEIKKNILKKTKSFNSKKKETVKSKSKSPIDFFDETKRGVFIVPPETDILSRRELNQKTVVNTVPNQTSSYPTINENKLVELNNQPETKVLETKKDSFTTTIREKKLNPEDSQAFWYIFVGDRKYGFWKNHTWVWLGYFDQLQRWNYFKVIETVEVPQEHAAFIKQRPADIDFWRPLVGNPNYGFVQNNTWIWKGFFDKKLNWIPDPVRFTEEALGHTDSLVDEIEKKTISEQPYWEQENDIVVTVFNTKSLASSLENELLLENSSEEQPVIEEVKPRRNEVIFRNPVTKLHFEKEKFEFLNPVKETNETIPLIEIVKEEVKVESEVEAPVEIEPEAACEPETTIPEVETVFVYEDDLKGLDSNQTQAGNVPEVETVFVYEDDLKGLDSIIKDDQQHDEIAKHVEHLSQDYSKEIKDSAKADLSNISDDIDSVWKEFGSFTDETQKSVEEKSQVDEIILDANNDFINESLFRDEVVNNIDSQINETVSEQQFEPTYSVNEFQQEFSEPVVSDEKIKETNSDESVNTDLTALFSEKLVNEVLLTNEYVDVNAPFSTETEVKVSSELPKSELVDEITFINNDPKPQEGLEYKVDFLETEPKSLFDEKTTIVVESEPPFIQPDLSLELDSVNDVDKSLETKTTSVELNHEEIGNEFINLDVSEKEVQEQPTTQLETDSEFVLPTYQIVEDSFTESAETPNEFSSEQKDTLEFISQTQEVETSESNVPTVEQETKLFEHQDENNLFTPLPLDLTEIIESNALFDSKPDEKESSDSELQPTFKEIKLDSTVEVPQESSQVEATFDTVQPEAVFDEIKTQELQPEATTEVVFDDHFQPDVQPEQTPQEAKFDSPVEIPQESSQAEFHAEQISDEIKLEEKTEAVFDHQQLENQSEETVVTPTEVTAFEPETIETQLEPSSEDQPSEPALDQNHPEIVTAEVEQIFDGTKLEDLKLEEANFDNVENNEVQPKETEAEITFDETKELQQETSSEPLSTEELKSEATFDNVSEAESEAVFEKPQLETQTEKILEEEPKSEPVDQLITEASFDTVKHEAVFDKNQTQTEGLEEPQVSSEAEVVDQTTTDTVGEPEAVFDVQPEKTTEVKFDDVENQQKVISEPQVEQQPGEAVFEPSAEAKFDSPVESVQDSQPEPVLEEVQTQPEIQPVESQPEATFDTVQPEQTPQEAKFDSPVETVEQPEFSSEPTQQHVESEASFDEPNYDFDEPNYDFDQPSYDSDLQPSEPQYDVDEPNYDFDEPNYEIESKPSEPQFEPQVEQQPGEAVFEPSAEAKFDSPVESVQDSQPEPLLEEVQTQPEIQPVESQPEATFDTVQPEQTPQEAKFDSPVETIQEPQVSSEPEVVVQPNFEERKPETVLEEPQADEIQPEASEEESLDWELLVGNNSYGHYEPDGEWVWAGFFGDDQKWNKDATVKWARERDYLPLIGDEVYGRYNNKGEWIWYGFYDESGDWVLVDEQWKNRQPRINEAPKFWEKLIGNEEYGYYEDNEWNWYDGEFDSEGNWLVFQSEETENLNEDITKDIPALEGYDIDSIDADEWLSQFSADDAKDVFGSNDKK" }, "paralog": false, "identity": 0.9840319361277445, "score": 1450.0, "evalue": 0.0, "description": "Internal stop codon at 484404, 484446, 484467, 484575, 484599, 484629, 484635, 484689. Nonsense mutation around 484404, 484446, 484467, 484575, 484599, 484629, 484635, 484689.", "psc": { "uniref90_id": "UniRef90_Q49429", "product": "Protein P200", "uniref50_id": "UniRef50_Q49429", "go_ids": [ "GO:0020035" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49429", "UniRef:UniRef50_Q49429", "GO:0020035" ] }, "pscc": { "uniref50_id": "UniRef50_Q49429", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49429" ], "product": "Protein P200" } }, "id": "KMADJIBOEG_890", "locus": "GCA000292445_00890" }, { "type": "cds", "sequence": "contig_1", "start": 487151, "stop": 487348, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGAAGTTCACTTGAAACCTTAACTTCTGTTTCTGTTGAAAAAGGAGCATTAACATCAACATATTCATTTGCTAAGAGTACCTCATTAACAAGTTTTTCACTAAATAAAGCAGTTAAATCAGTATTAACACTTTCATCACTGTTTGTTTCTTTTATCTTTTCATCTGAAACAACAGGTTCGCTAAATTCTTGTTGA", "aa": "MGSSLETLTSVSVEKGALTSTYSFAKSTSLTSFSLNKAVKSVLTLSSLFVSFIFSSETTGSLNSC", "aa_hexdigest": "833eba00ebd4cd3b6ec72d62f4db5a07", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6768.577899999998, "isoelectric_point": 7.899843025207519 }, "id": "KMADJIBOEG_891", "locus": "GCA000292445_00891" }, { "type": "cds", "sequence": "contig_1", "start": 487454, "stop": 487621, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATCATTATTAGCATCTAAAATAATTTCATCTACTTGTGATTTTTCTTCTACACTTTTTTGTGTTTCATCAGTAAAAGAACCGAATTCTTTTCAAACTGAATCAATATCATCAGAAATATTAGATAAATCTGCTTTAGCACTATCTTTTATTTCTTTAGAATAA", "aa": "MKSLLASKIISSTCDFSSTLFCVSSVKEPNSFQTESISSEILDKSALALSFISLE", "aa_hexdigest": "092228fd981c62e73b5e6ecaad045e11", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5921.717399999998, "isoelectric_point": 4.8005872726440435 }, "id": "KMADJIBOEG_892", "locus": "GCA000292445_00892" }, { "type": "cds", "sequence": "contig_1", "start": 487919, "stop": 488203, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTCATTAGTTTCTTTAACTGGATTTAAGAATTCAAATTTTTCTTTTTCAAAATGTAATTTAGTTACTGGATTACGGAAGATAACTTCATTTCTTCTTGGTTTAACTTCTTCAATTACCGGCTGCTCTTCACTTGAATTTTCAAGTAATAGTTCGTTTTCTAGAGAAGAAGCTAAGCTCTTGGTATTAAATACTGTAACAACAATGTCATTTTCCTGTTCTCAGTATGGTTGTTCAGAAATAGTCTTTTTCTCTATTTCATCAACTAAGCTATCAGTATGA", "aa": "MVSLVSLTGFKNSNFSFSKCNLVTGLRKITSFLLGLTSSITGCSSLEFSSNSSFSREEAKLLVLNTVTTMSFSCSQYGCSEIVFFSISSTKLSV", "aa_hexdigest": "4efbb505a0c6d021d68f16798ce2e917", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10122.533399999993, "isoelectric_point": 8.836504173278808 }, "id": "KMADJIBOEG_893", "locus": "GCA000292445_00893" }, { "type": "cds", "sequence": "contig_1", "start": 488503, "stop": 488997, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAAAACAACAAAGAATAAAAACAAAAACACCACCCCTAAATCAAAAACCAAAAAGTATTTAGAATCAGCTAACAAGAAGAGTGTTACTAAACCAAAAAAAGAACAAGATAAAGTTGAAAATCTTTTTGATCAACCTTTTTTAGGTGAAATCAAAAAAAACATTCTCAAGAAAACAAAGTCTTTTAATAGTAAGAAAAAGGAAACAGTTAAATCAAAGAGCAAATCACCTATAGATTTTTTTGATGAAACCAAGCGCGGTGTTTTTATTGTTCCACCTGAAACAGATATCTTAAGTAGAAGAGAACTTAATCAAAAAACTGTTGTTAATACAGTTCCAAATCAAACAAGCAGTTATCCAACCATTAATGAAAATAAGTTGGTTGAATTAAACAATCAACCAGAAACCAAAGTTCTAGAAACAAAAAAAGATAGTTTTACAACAACTATTAGAGAAAAAAAATTAAATCCAGAAGATTCACAAGCGTTCTGA", "aa": "MPKTTKNKNKNTTPKSKTKKYLESANKKSVTKPKKEQDKVENLFDQPFLGEIKKNILKKTKSFNSKKKETVKSKSKSPIDFFDETKRGVFIVPPETDILSRRELNQKTVVNTVPNQTSSYPTINENKLVELNNQPETKVLETKKDSFTTTIREKKLNPEDSQAF", "aa_hexdigest": "4710bbf8d9536695c6d5ba638c063d09", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18893.3926, "isoelectric_point": 9.87360706329346 }, "id": "KMADJIBOEG_894", "locus": "GCA000292445_00894" }, { "type": "cds", "sequence": "contig_1", "start": 488997, "stop": 489086, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGCTAACTTTTGTTATCTTGATATTAAAAATTACAATTTAATGAGTCAGATAACTTTAGTCACTAGTGCTAAGTATTTTTATTAA", "aa": "MYANFCYLDIKNYNLMSQITLVTSAKYFY", "aa_hexdigest": "da00ca37052868d535c79ea643f54e8a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3519.0699999999997, "isoelectric_point": 7.867093086242677 }, "id": "KMADJIBOEG_895", "locus": "GCA000292445_00895" }, { "type": "cds", "sequence": "contig_1", "start": 489282, "stop": 489485, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07650.23" ], "nt": "TTGTTAAAAATTCACCTAGTAATTAGTGTTCCAAAATTAAGCCAAAAAAAGATTATCATTGGAAAAAATGCAGAAATGATCAAAGCAATTGGGATAGCAACTCGCAAAAAACTATTAAATCATTTTGACTGTGATATTTTTACTGATATTTTTGTAAAAACAGAAAAACAAAAATTGCCTGTTTATAGTTTTCTTTCTAAATAA", "aa": "MLKIHLVISVPKLSQKKIIIGKNAEMIKAIGIATRKKLLNHFDCDIFTDIFVKTEKQKLPVYSFLSK", "aa_hexdigest": "dd125656d00574b63600e5c16e7eb6d7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07650.23", "name": "KH_2", "length": 58, "aa_cov": 0.8059701492537313, "hmm_cov": 0.9827586206896551, "evalue": 3.668446962480065e-10, "score": 34.713199615478516, "start": 4, "stop": 57 } ], "seq_stats": { "molecular_weight": 7685.295099999999, "isoelectric_point": 9.945747184753419 }, "id": "KMADJIBOEG_896", "locus": "GCA000292445_00896" }, { "type": "cds", "sequence": "contig_1", "start": 489675, "stop": 490154, "strand": "-", "frame": 2, "gene": "era", "product": "GTPase Era", "db_xrefs": [ "COG:COG1159", "COG:J", "EC:3.6.5.-", "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0019843", "GO:0043024", "GO:0070181", "SO:0001217", "UniRef:UniRef50_P47627", "UniRef:UniRef90_P47627" ], "nt": "ATGAAAGTTTTAAAAGTTGGTGTTTTAGGACCTACTAATGCAGGAAAATCAACGCTAATTAATTTTTTACATAACGATGATAGTCTGATGGTATCATCCATGAATAACACAACGCTTCTTTCTATTTCCACTGAGGTAATTAACCAAGCTAATAAAAATATTGTTTTTATTGATGTCCCTGGTTTTACTGAGAAAAAACATTCAAATTATGAATTAATAACTAAAGAAATTCGCAAAGCATTAAGTGGAATTGATGTATTGCTTTTAGTTGTTAGAAGTGATCAGAATAACAAGATAGAATTTTTAAAAACTCAATTACAACAATTAAAGCGCTATCAAAATTTAACAAGAATTTTTTTAATTAATAAATTTCATCAAAAAAGTTTAAGTGAAGTTAATAAAGCAATTATTTTAGAAGAGTTTAAACCACAAAAAACAATTGAAATAAACTTACTTAAGTTTGATAAAAACCTCTTTTGA", "aa": "MKVLKVGVLGPTNAGKSTLINFLHNDDSLMVSSMNNTTLLSISTEVINQANKNIVFIDVPGFTEKKHSNYELITKEIRKALSGIDVLLLVVRSDQNNKIEFLKTQLQQLKRYQNLTRIFLINKFHQKSLSEVNKAIILEEFKPQKTIEINLLKFDKNLF", "aa_hexdigest": "fe69406db23df748a8201ca25f056780", "start_type": "ATG", "rbs_motif": null, "genes": [ "era" ], "truncated": "5-prime", "pseudogene": { "start": 489372, "stop": 490154, "observations": { "mutation": [ 489677 ], "stop": [ 489677 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47627", "query_cov": 1.0, "subject_cov": 0.5482758620689655, "identity": 1.0, "score": 300.0, "evalue": 8.66e-101, "gene_start": 1, "gene_end": 159, "reference_start": 1, "reference_end": 159, "reference_sequence": "MKVLKVGVLGPTNAGKSTLINFLHNDDSLMVSSMNNTTLLSISTEVINQANKNIVFIDVPGFTEKKHSNYELITKEIRKALSGIDVLLLVVRSDQNNKIEFLKTQLQQLKRYQNLTRIFLINKFHQKSLSEVNKAIILEEFKPQKTIEINLLKFDKNLFWSIFKQVELRYNIFRKDINFIDANNDDFKILEGLREQIIFYCKNEIPHIARIEIIEKSFNKEKNLLKIHLVISVPKLSQKKIIIGKNAEMIKAIGIATRKKLLNHFDCDIFIDIFVKTEKQKLPVYSFLSK" }, "paralog": false, "identity": 0.9961538461538462, "score": 498.0, "evalue": 1.24e-181, "description": "Internal stop codon at 489677. Nonsense mutation around 489677.", "psc": { "uniref90_id": "UniRef90_P47627", "gene": "era", "product": "GTPase Era", "ec_ids": [ "3.6.5.-" ], "uniref50_id": "UniRef50_P47627", "cog_id": "COG1159", "cog_category": "J", "go_ids": [ "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0019843", "GO:0043024", "GO:0070181" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47627", "EC:3.6.5.-", "UniRef:UniRef50_P47627", "COG:COG1159", "COG:J", "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0019843", "GO:0043024", "GO:0070181" ] }, "pscc": { "uniref50_id": "UniRef50_P47627", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47627" ], "product": "GTPase Era" } }, "id": "KMADJIBOEG_897", "locus": "GCA000292445_00897" }, { "type": "cds", "sequence": "contig_1", "start": 490283, "stop": 490606, "strand": "-", "frame": 3, "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "db_xrefs": [ "COG:COG0319", "COG:J", "EC:3.1.-.-", "GO:0004222", "GO:0004521", "GO:0004540", "GO:0005737", "GO:0006364", "GO:0008270", "GO:0042254", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47628", "UniRef:UniRef90_P47628" ], "nt": "TTGAAGTCATCTTTTAGCATTAATTCAAGCCGCCTTTTTAAGCGCTTTTTTGGTAAAAATTACCAAGCAGGAGTCGAACTTTGTTTCCAAATTATTAAAAGCAGTTTAAACCTATCGTTTGACCCAGAATTTGCGCTTTTAATTGTTTCCTGTTCAAAAATGAAAAAACTGAACAAACAGTTTTTAAAACGTAGAGGTTGTACTGATGTAATTAGTTTGTGCTATAACGAAACTGAAACCGGTTTTTCACTAGCAATCGGCGAAATTTTTTTGTGTCCTAAATACATCTTTAAAAAAGCTAAAGAGATTGGTTGTAACAATTGA", "aa": "MKSSFSINSSRLFKRFFGKNYQAGVELCFQIIKSSLNLSFDPEFALLIVSCSKMKKLNKQFLKRRGCTDVISLCYNETETGFSLAIGEIFLCPKYIFKKAKEIGCNN", "aa_hexdigest": "09db2ba6b472e48feac7092f8ce790c2", "start_type": "TTG", "rbs_motif": null, "genes": [ "ybeY" ], "truncated": "5-prime", "pseudogene": { "start": 490142, "stop": 490606, "observations": { "mutation": [ 490153, 490285 ], "stop": [ 490153, 490285 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47628", "query_cov": 1.0, "subject_cov": 0.6948051948051948, "identity": 1.0, "score": 218.0, "evalue": 1.45e-71, "gene_start": 1, "gene_end": 107, "reference_start": 1, "reference_end": 107, "reference_sequence": "MKSSFSINSSRLFKRFFGKNYQAGVELCFQIIKSSLNLSFDPEFALLIVSCSKMKKLNKQFLKRRGCTDVISLCYNETETGFSLAIGEIFLCPKYIFKKAKEIGCNNWFLLTRCLIHGILHLFEFNHEESEAFESVTMFFQDAIHEEILSVWKF" }, "paralog": false, "identity": 0.9805194805194806, "score": 304.0, "evalue": 6.49e-108, "description": "Internal stop codon at 490153, 490285. Nonsense mutation around 490153, 490285.", "psc": { "uniref90_id": "UniRef90_P47628", "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P47628", "cog_id": "COG0319", "cog_category": "J", "go_ids": [ "GO:0004222", "GO:0004521", "GO:0004540", "GO:0005737", "GO:0006364", "GO:0008270", "GO:0042254", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47628", "EC:3.1.-.-", "UniRef:UniRef50_P47628", "COG:COG0319", "COG:J", "GO:0004222", "GO:0004521", "GO:0004540", "GO:0005737", "GO:0006364", "GO:0008270", "GO:0042254", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47628", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47628" ], "product": "Endoribonuclease YbeY" } }, "id": "KMADJIBOEG_898", "locus": "GCA000292445_00898" }, { "type": "cds", "sequence": "contig_1", "start": 490644, "stop": 490838, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MG389", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_P47629", "UniRef:UniRef90_P47629" ], "nt": "ATGTTTAAAAATGAAAAAGATGAAAATCTTTTTAACAACGTTTCATGGACTAATTTTCAAGCAGAAACAACAGAAAAATCAGCAAAAAAAGCTTTTAGACTTTACAAAAAGAAAATTCCTACATCAGAAATTTCACCAGAATTGAACAAAAATCTAGTAAAGGTAGATTTAAGTGTTACTCTAGAACAGGGCTGA", "aa": "MFKNEKDENLFNNVSWTNFQAETTEKSAKKAFRLYKKKIPTSEISPELNKNLVKVDLSVTLEQG", "aa_hexdigest": "9e792afe32b1e6dbe6d96ae2bb9c2ff2", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 490590, "stop": 490973, "observations": { "mutation": [ 490646, 490865 ], "stop": [ 490646, 490865 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47629", "query_cov": 1.0, "subject_cov": 0.5039370078740157, "identity": 0.9690000000000001, "score": 117.0, "evalue": 1.2e-32, "gene_start": 1, "gene_end": 64, "reference_start": 46, "reference_end": 109, "reference_sequence": "MKQKILGITIAFIILLLTTVAILFSVKVKQYLNVNLWTTQQQNFLMFKNEKDENLFNNVSWTNFQAETTEKSAKKAFRLYKKKISTSEISSELNKNLVKVDLSVTLEQGWYNITIMLPSKDLFEVIF" }, "paralog": false, "identity": 0.968503937007874, "score": 234.0, "evalue": 3.54e-81, "description": "Internal stop codon at 490646, 490865. Nonsense mutation around 490646, 490865.", "psc": { "uniref90_id": "UniRef90_P47629", "product": "Uncharacterized protein MG389", "uniref50_id": "UniRef50_P47629", "go_ids": [ "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47629", "UniRef:UniRef50_P47629", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47629", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47629" ], "product": "Uncharacterized protein MG389" } }, "id": "KMADJIBOEG_899", "locus": "GCA000292445_00899" }, { "type": "cds", "sequence": "contig_1", "start": 490863, "stop": 490973, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGCAGAAAATTTTAGGAATAACAATTGCTTTTATTATTTTGCTTTTAACAACAGTTGCTATTTTGTTTTCAGTAAAGGTAAAACAATATCTTAATGTAAATCTTTGA", "aa": "MKQKILGITIAFIILLLTTVAILFSVKVKQYLNVNL", "aa_hexdigest": "46036968c72f159cecceacf64166a32e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4061.0538000000006, "isoelectric_point": 10.176350593566895 }, "id": "KMADJIBOEG_900", "locus": "GCA000292445_00900" }, { "type": "cds", "sequence": "contig_1", "start": 490977, "stop": 491324, "strand": "-", "frame": 2, "gene": null, "product": "ABC-type enterochelin transport system, ATPase component", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAV5N885" ], "nt": "ATGCAAACAGAAGCAAATGATTTTGGCTTTAGTGAGGCTTTATTTAATAATCAGAATCCACATATCTATCAGTTACTTTTTGATGCAGGTGTTCAAAATCAAACAAAGTTAAGTTCAGGACAAAAACAAATTTTACAACTATTCTTACTTAGCAACATTAAGAATAAGGTGATCTTACTTGATGAATGCATGAATGCAATAGCACCTGAAATTAAAAACCGTGTTTATCAGCTATTAGTAAAACCACTTACTTTAAATAATTTTGTAGTCTTAGTTGAGCATGATCTGAGTTTTGCAAGTGAAGCTCAAAACAAGATTAACCTAACTAATTACTTAAGAAATAGTTAA", "aa": "MQTEANDFGFSEALFNNQNPHIYQLLFDAGVQNQTKLSSGQKQILQLFLLSNIKNKVILLDECMNAIAPEIKNRVYQLLVKPLTLNNFVVLVEHDLSFASEAQNKINLTNYLRNS", "aa_hexdigest": "d9e4a607d87bdad315209b09af887035", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAV5N885", "query_cov": 0.8695652173913043, "subject_cov": 0.9803921568627451, "identity": 0.67, "score": 132.0, "evalue": 3.65e-38, "valid": false, "product": "ABC-type enterochelin transport system, ATPase component", "uniref50_id": "UniRef50_A0AAV5N885" }, "pscc": { "uniref50_id": "UniRef50_A0AAV5N885", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAV5N885" ], "product": "ABC-type enterochelin transport system, ATPase component" }, "genes": [], "id": "KMADJIBOEG_901", "locus": "GCA000292445_00901" }, { "type": "cds", "sequence": "contig_1", "start": 491586, "stop": 492266, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAACAAAAAAGCTTTGTTTACTTTAGTAATAAATCACCAAATCAATTAATAGAACGACAATTTTATTTAAAAGAAGTTCTTTCCTTTTTTAACTTTTATATTCCCAATTTAATTATTAGTTGTGTTGTTGCACTAATTATCGGTGTTTTAATAGGAATTAATCAATTAGAATTTTTATTGATTGCCATTGCACAAATAGTAGTCAATGCTGGCTTATTTTGTTATGACTTCTTTTTTACTAAAAAAATTACAAAGAAGGAAATTCCTTATGTTGAATTGCAAAACAAAATTAGTTTACAGCTAGATGAAAATTTGCGTGAAGAACAAAATAAAAAGCGCTTTAATTTCTTAATGTTGAATTTTCGTAAAGCACTTTTACAAAATCAAAACATTAACAACCAGAAGGAAATTAATCGTCTAACTATAGAAAACATTAAAAGCTTCTTCCAACAAGGTTTTGATTTTGCTATATTAGGTTTGGGGGTGATTGGTATTATTGAACAGCGTTATCAATTGAGCTTTTTATTCTATGTTTTTGGTATCCAATCACTTTTTTCAACTTATGCTACAAGAATTGTTCAATTTGGTGCTGCAATAAACATTTATCACTATTGTCGTGAGAAATTAGTTAACTTGTTTATAGAAACCAAAAAGGATGAGGGTATTAAAGTAAATTGA", "aa": "MQQKSFVYFSNKSPNQLIERQFYLKEVLSFFNFYIPNLIISCVVALIIGVLIGINQLEFLLIAIAQIVVNAGLFCYDFFFTKKITKKEIPYVELQNKISLQLDENLREEQNKKRFNFLMLNFRKALLQNQNINNQKEINRLTIENIKSFFQQGFDFAILGLGVIGIIEQRYQLSFLFYVFGIQSLFSTYATRIVQFGAAINIYHYCREKLVNLFIETKKDEGIKVN", "aa_hexdigest": "2ba9b9a482bff619f5503df6163b3eb5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26553.785900000013, "isoelectric_point": 9.24955310821533 }, "id": "KMADJIBOEG_902", "locus": "GCA000292445_00902" }, { "type": "cds", "sequence": "contig_1", "start": 492666, "stop": 492959, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATCATTTATCAAGAACAACAGAATGAGTGTGGAATTTGCGTAATTGGTATGCTTGCCAATGCAATCCATGATGAAAAGTATGTTCATGATGAATTACTAGAACAAATTAACTTACCACCAAATGGCTTAAGCTTATTTGAAATGGAAAGTTATGGCAAAAAGTTTGGTCTTGAAATCAACAGTTATCAATTAACATTTCAAGAACTAAAAGAACTTGATAGCAAATTCATAATAGTTCATTTTAAAGATCACTTTGTCATAGTTAAAAACAAACATGAAAATAGTTGA", "aa": "MKIIYQEQQNECGICVIGMLANAIHDEKYVHDELLEQINLPPNGLSLFEMESYGKKFGLEINSYQLTFQELKELDSKFIIVHFKDHFVIVKNKHENS", "aa_hexdigest": "2f88fd873904c40c1cdda9a37a3c97f8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11357.908999999987, "isoelectric_point": 5.241998863220213 }, "id": "KMADJIBOEG_903", "locus": "GCA000292445_00903" }, { "type": "cds", "sequence": "contig_1", "start": 493067, "stop": 493300, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00883.26" ], "nt": "ATGCACCCTGATTATTTAAAACCTTTACAGCTAACAAAACTTGCTGATTTGCAAAATTCTACTAGTGCAAGAGGTGCTGGATCTTCAAGAGCAGCTTGTTTCCTTGCAGAATTTAGAGAAGGTGTATCTTTGATCCATTGTGATATTGCATCAACTGCTTCCATTGAGAACCTTGGACAAGGTGTTTTAGTGAGAACCTTGTACGAACGTGCTAGTCAGCTTGCAAATAAATAA", "aa": "MHPDYLKPLQLTKLADLQNSTSARGAGSSRAACFLAEFREGVSLIHCDIASTASIENLGQGVLVRTLYERASQLANK", "aa_hexdigest": "35eaeb6e96a5625f289f9cfdcb7639f7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00883.26", "name": "Peptidase_M17", "length": 74, "aa_cov": 0.8571428571428571, "hmm_cov": 0.972972972972973, "evalue": 1.4621758600449746e-10, "score": 35.99742126464844, "start": 2, "stop": 67 } ], "seq_stats": { "molecular_weight": 8305.357199999993, "isoelectric_point": 7.827573966979982 }, "id": "KMADJIBOEG_904", "locus": "GCA000292445_00904" }, { "type": "cds", "sequence": "contig_1", "start": 493355, "stop": 494410, "strand": "-", "frame": 3, "gene": "pepA", "product": "leucyl aminopeptidase", "db_xrefs": [ "COG:COG0260", "COG:E", "EC:3.4.11.1", "GO:0005737", "GO:0006508", "GO:0008233", "GO:0030145", "GO:0070006", "SO:0001217", "UniRef:UniRef50_P47631", "UniRef:UniRef90_P47631" ], "nt": "ATGAGAATAAATAAACCTTTTAGTGATGATAGCAACACAGTTGTTTTTGTGAGTTCAAAAACATATGGTGTAAAAGAAGAAGCTGCACATAATCCTAATGTTGAATTTGGTGTTGTTTTACCAACTGATTTTCCTGCTTTCAACCGTGCTTTAGTTCAATTTCTTAAAAGAAAGAAAACCAAATTAAACATTAATCTTGACAGTCTTATAGAACTTTATAAGAAAAATGAAAATAGTGGTTGTTTTCATACTGCGATAAAAACTGTTATTACAAGTGTTACTTTTTGTGAAACTACTCCTTTCACAATGAAAACTAAACCTGAAAAAAATGTTGAAGTTGCTGTTCAATGTGCTGTTGAATATCACAACTTAGTTAAAGAATATGAAACAGTAGGCGAATATGTTAACCTAGCAAGAGAATTACAAGACACTCCTTCAGATCTACTTTATCCAGAAGTATTTGTTAAACATTTTGAAAAGGCTGCAAGTAAATTGCCTGTAAAAATAAAAGTTCTTAAACAATCAGATCTAATTAAGAAAAAAATGGGTTTACTTTTAGGGGTTAATCAAGGCTCTGAAAGAGAAGCACGTTTACTTGTTATTAGTTATCAAGCTAATAAAAATTCCAAAGAAAAACTTGCTTTTGTAGGGAAAGGAATTACTTATGATTCAGGCGGAATGAACATTAAAACAGGTGATTATATGCGTGGCATGAAATATGACATGAGTGGTGCGGCTATAGTATGTTCTACTGTTTTGGCATTAGCTAAAAACAAGGTTAAAACCAATGTTGTTGCAGTAGCAGCTCTTACTGAAAATCTTCCTGGTGCTAAGGCGCAACGTCCTGATGACATTAAGATAGCATACAATGGTAAAAGTGTTGAAATAGATAACACTGATGCTGAAGGAAGATTGGTTTTAGCTGATGCTATTACATATGCTGCTAAGGATTTAGCTGCTACACATATTATTGATGTAGCAACCCTTACTGGTTTAATGTCATACATATTGAGTACTACCTATACAGGTATTTTCAGTACTTGTGATCACCAGTGA", "aa": "MRINKPFSDDSNTVVFVSSKTYGVKEEAAHNPNVEFGVVLPTDFPAFNRALVQFLKRKKTKLNINLDSLIELYKKNENSGCFHTAIKTVITSVTFCETTPFTMKTKPEKNVEVAVQCAVEYHNLVKEYETVGEYVNLARELQDTPSDLLYPEVFVKHFEKAASKLPVKIKVLKQSDLIKKKMGLLLGVNQGSEREARLLVISYQANKNSKEKLAFVGKGITYDSGGMNIKTGDYMRGMKYDMSGAAIVCSTVLALAKNKVKTNVVAVAALTENLPGAKAQRPDDIKIAYNGKSVEIDNTDAEGRLVLADAITYAAKDLAATHIIDVATLTGLMSYILSTTYTGIFSTCDHQ", "aa_hexdigest": "1fe2edb75aa2c4cca60684d99bdb9b00", "start_type": "ATG", "rbs_motif": null, "genes": [ "pepA" ], "truncated": "5-prime", "pseudogene": { "start": 493067, "stop": 494410, "observations": { "mutation": [ 493312, 493357 ], "stop": [ 493312, 493357 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47631", "query_cov": 1.0, "subject_cov": 0.785234899328859, "identity": 0.997, "score": 671.0, "evalue": 1.4e-241, "gene_start": 1, "gene_end": 351, "reference_start": 1, "reference_end": 351, "reference_sequence": "MRINKPFSDDSNTVVFVSSKTYGVKEEAAHNPNVEFGVVLPTDFPAFNRALVQFLKRKKTKLNINLDSLIELYKKNENSGCFHTAIKTVITSVTFCETTPFTMKTKPEKNVEVAVQCAVEYHNLVKEYETVGEYVNLARELQDTPSDLLYSEVFVKHFEKAASKLPVKIKVLKQSDLIKKKMGLLLGVNQGSEREARLLVISYQANKNSKEKLAFVGKGITYDSGGMNIKTGDYMRGMKYDMSGAAIVCSTVLALAKNKVKTNVVAVAALTENLPGAKAQRPDDIKIAYNGKSVEIDNTDAEGRLVLADAITYAAKDLAATHIIDVATLTGLMSYILSTTYTGIFSTCDHQWESFKKAACSAGEPVWRLPMHPDYLKPLQLTKLADLQNSTSARGAGSSRAACFLAEFREGVSLIHCDIASTASIENLGQGVLVRTLYERASQLANK" }, "paralog": false, "identity": 0.9932885906040269, "score": 866.0, "evalue": 3.94e-321, "description": "Internal stop codon at 493312, 493357. Nonsense mutation around 493312, 493357.", "psc": { "uniref90_id": "UniRef90_P47631", "gene": "pepA", "product": "leucyl aminopeptidase", "ec_ids": [ "3.4.11.1" ], "uniref50_id": "UniRef50_P47631", "cog_id": "COG0260", "cog_category": "E", "go_ids": [ "GO:0005737", "GO:0030145", "GO:0070006", "GO:0008233", "GO:0006508" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47631", "EC:3.4.11.1", "UniRef:UniRef50_P47631", "COG:COG0260", "COG:E", "GO:0005737", "GO:0030145", "GO:0070006", "GO:0008233", "GO:0006508" ] }, "pscc": { "uniref50_id": "UniRef50_P47631", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47631" ], "product": "Probable cytosol aminopeptidase" } }, "id": "KMADJIBOEG_905", "locus": "GCA000292445_00905" }, { "type": "cds", "sequence": "contig_1", "start": 494427, "stop": 496058, "strand": "-", "frame": 2, "gene": "groL", "product": "chaperonin GroEL", "db_xrefs": [ "COG:COG0459", "COG:O", "EC:5.6.1.7", "GO:0005524", "GO:0009408", "GO:0016853", "GO:0042026", "GO:0051082", "GO:0051085", "GO:0140662", "GO:1990220", "RefSeq:WP_009885631.1", "SO:0001217", "UniParc:UPI0000557C61", "UniRef:UniRef100_P47632", "UniRef:UniRef50_P47632", "UniRef:UniRef90_P47632" ], "nt": "ATGGCAAAGGAATTAATCTTTGGTAAAGATGCGAGAACCCGCTTGTTGCAGGGTATTAATAAGATAGCAAATGCTGTTAAAGTAACAGTAGGTCCTAAAGGCCAAAATGTTATTTTAGAGAGAAAATTTGCAAATCCATTAATTACTAACGATGGGGTTACAATCGCAAAAGAAATAGAACTTAGTGATCCAGTTGAAAATATTGGTGCTAAGGTTATTTCAGTTGCTGCAGTGTCAACTAATGACATTGCTGGGGATGGTACAACAACAGCTACCATATTAGCACAAGAAATGACAAACCGTGGTATTGAAATTATCAATAAAGGTGCTAATCCTGTTAACATCCGCAGGGGTATTGAAGATGCAAGCTTACTTATTATTAAAGAACTTGAAAAGTACTCTAAAAAAATTAATACTAACGAAGAGATAGAACAAGTTGCAGCTATCTCTTCAGGTTCTAAAGAAATTGGTAAACTGATCGCTCAAGCAATGGCTTTAGTTGGTAAAAATGGCGTGATAACAACTGATGATGCAAAAACCATTAATACAACATTAGAAACCACTGAAGGAATTGAATTTAAAGGAACATATGCATCACCTTATATGGTTAGTGATCAAGAAAAAATGGAAGTTGTTTTAGAACAACCTAAAATCTTAGTAAGCTCTTTAAAAATTAACACAATTAAAGAAATTCTTCCGCTTTTAGAAGGTAGTGTTGAAAATGGTAATCCATTATTAATTGTTGCACCTGACTTTGCAGAAGAAGTTGTTACTACTTTAGCAGTTAATAAACTCAGGGGCACCATTAATGTTGTTGCTGTTAAATGTAATGAATATGGTGAACGTCAAAAAGCAGCTTTAGAAGATTTAGCAATTAGTAGTGGAACCTTAGCATATAATAACGAAATTAATAGTGGTTTTAAAGATGTTACTGTTGATAATTTAGGTGATGCTAGAAAGGTTCAAATAGCTAAAGAAAAAACTACTGTTATTGGTGGTAAAGGCAATAAGGATAAAATCAAAAAGCATGTTGAACTTCTAAACGGAAGATTAAAACAAACCACTGACAAGTATGATTCTGATTTAATTAAAGAAAGAATTGCTTATTTAAGTCAAGGTGTTGCTGTTATCCGTGTTGGTGGTGCAACTGAACTCGCACAAAAAGAATTAAAACTCAGAATTGAAGACGCTTTAAATTCCACCAAAGCTGCAGTTGAAGAAGGGATTATCGCTGGAGGTGGTGTTGGTTTATTAAATGCTTCTTGTGTTTTAACTAACAGTAAACTAAAAGAACGATATGAAAATGAAACTAGTGTTGAAAACATTAAAGAAATCCTACTTGGTTTTGAAATTGTGCAAAAGTCTCTAGAAGCACCAGCGCGTCAAATTATTCAAAACTCAGGAGTTGACCCAGTTAAAATTCTCAGTGAATTGAAAAATGAAAAAACTGGTGTTGGCTTTGATGCTGAGACTAAAAAGAAGGTTGATATGATTGCAAATGGAATCATTGATCCCACCAAAGTAACTAAAACTGCACTTGAAAAAGCTGCTTCTGTAGCTAGTTCATTAATTACTACTAATGTTGCTGTGTATGATGTTAAAGAGAGAAAAGATAACTCCTTTTCAGAATAA", "aa": "MAKELIFGKDARTRLLQGINKIANAVKVTVGPKGQNVILERKFANPLITNDGVTIAKEIELSDPVENIGAKVISVAAVSTNDIAGDGTTTATILAQEMTNRGIEIINKGANPVNIRRGIEDASLLIIKELEKYSKKINTNEEIEQVAAISSGSKEIGKLIAQAMALVGKNGVITTDDAKTINTTLETTEGIEFKGTYASPYMVSDQEKMEVVLEQPKILVSSLKINTIKEILPLLEGSVENGNPLLIVAPDFAEEVVTTLAVNKLRGTINVVAVKCNEYGERQKAALEDLAISSGTLAYNNEINSGFKDVTVDNLGDARKVQIAKEKTTVIGGKGNKDKIKKHVELLNGRLKQTTDKYDSDLIKERIAYLSQGVAVIRVGGATELAQKELKLRIEDALNSTKAAVEEGIIAGGGVGLLNASCVLTNSKLKERYENETSVENIKEILLGFEIVQKSLEAPARQIIQNSGVDPVKILSELKNEKTGVGFDAETKKKVDMIANGIIDPTKVTKTALEKAASVASSLITTNVAVYDVKERKDNSFSE", "aa_hexdigest": "5502f1565b9d7b37257e1363472a1713", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000557C61", "ncbi_nrp_id": "WP_009885631.1", "uniref100_id": "UniRef100_P47632", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000557C61", "RefSeq:WP_009885631.1", "UniRef:UniRef100_P47632" ] }, "ips": { "uniref100_id": "UniRef100_P47632", "uniref90_id": "UniRef90_P47632" }, "psc": { "uniref90_id": "UniRef90_P47632", "gene": "groL", "product": "chaperonin GroEL", "ec_ids": [ "5.6.1.7" ], "uniref50_id": "UniRef50_P47632", "cog_id": "COG0459", "cog_category": "O", "go_ids": [ "GO:0005524", "GO:0009408", "GO:0016853", "GO:0042026", "GO:0051082", "GO:0051085", "GO:0140662", "GO:1990220" ] }, "pscc": { "uniref50_id": "UniRef50_P47632", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47632" ], "product": "Chaperonin GroEL" }, "genes": [ "groL" ], "id": "KMADJIBOEG_906", "locus": "GCA000292445_00906" }, { "type": "cds", "sequence": "contig_1", "start": 496071, "stop": 496403, "strand": "-", "frame": 2, "gene": "groES", "product": "co-chaperone GroES", "db_xrefs": [ "COG:COG0234", "COG:O", "GO:0005524", "GO:0005737", "GO:0044183", "GO:0046872", "GO:0051082", "GO:0051085", "GO:0051087", "RefSeq:WP_010869464.1", "SO:0001217", "UniParc:UPI0000127634", "UniRef:UniRef100_P47633", "UniRef:UniRef50_P47633", "UniRef:UniRef90_P47633" ], "nt": "ATGAACATAACGCCAATTCATGACAACGTCTTGGTTTCACTTGTGGAATCAAACAAAGAAGAAGTCTCAAAAAAAGGGATTATTACCTCATTGGCAAGTAATGATAAAAGCGATGCTAATGCTAATAAAGGGATTGTAATTGCTCTTGGTGCTGGTCCTGCATATGGCAAAACAGAAAAACCAAAATATGCTTTTGGTGTTGGTGATATTATTTACTTTAAGGAGTATAGTGGTATCTCTTTTGAGAATGAGGGAAACAAGTACAAAATTATTGGATTTGAGGATGTACTTGCCTTTGAAAAACCAGAAAGTGGTAAGCAAAGAAAAAGATAA", "aa": "MNITPIHDNVLVSLVESNKEEVSKKGIITSLASNDKSDANANKGIVIALGAGPAYGKTEKPKYAFGVGDIIYFKEYSGISFENEGNKYKIIGFEDVLAFEKPESGKQRKR", "aa_hexdigest": "00e270f14d81d7b2ac3f76a93d713102", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000127634", "ncbi_nrp_id": "WP_010869464.1", "uniref100_id": "UniRef100_P47633", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000127634", "RefSeq:WP_010869464.1", "UniRef:UniRef100_P47633" ] }, "ips": { "uniref100_id": "UniRef100_P47633", "uniref90_id": "UniRef90_P47633" }, "psc": { "uniref90_id": "UniRef90_P47633", "gene": "groES", "product": "co-chaperone GroES", "uniref50_id": "UniRef50_P47633", "cog_id": "COG0234", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0046872", "GO:0044183", "GO:0051087", "GO:0051082", "GO:0051085" ] }, "pscc": { "uniref50_id": "UniRef50_P47633", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47633" ], "product": "Co-chaperonin GroES" }, "genes": [ "groES" ], "id": "KMADJIBOEG_907", "locus": "GCA000292445_00907" }, { "type": "cds", "sequence": "contig_1", "start": 496529, "stop": 496855, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00464.25" ], "nt": "GTGTCAAAAGGTACTGAAACCCACTTATTTTCATTAGTGGTTGGTAATGGTAAAGATGTTGCGTTGTGGTTACAAAAAGCTAACATTGTTTTGAATATGAATACAATCCCTTTTGAAACAAAATCTGCTTTTAGTCCTTCAGGTATTAGACTTGGAACTCCTGCAATGACAACCAGAGGTTTTAAAACTAATGACTTTATTTTTGTTGCCAGTTTGATTGATAAGGTTATTAAAAGTAATGGTAATCAAAAGGTAATTAGTCAAACAAAAACAGCTGTTTTAAATCTCTTAAAACGCTTTCCGCTCTATAAGGGTTTAGCTTATTAA", "aa": "MSKGTETHLFSLVVGNGKDVALWLQKANIVLNMNTIPFETKSAFSPSGIRLGTPAMTTRGFKTNDFIFVASLIDKVIKSNGNQKVISQTKTAVLNLLKRFPLYKGLAY", "aa_hexdigest": "316b9f1848202e548b155262d8a54a9e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00464.25", "name": "SHMT", "length": 76, "aa_cov": 0.6666666666666666, "hmm_cov": 0.9868421052631579, "evalue": 1.2021411161078347e-17, "score": 58.72493362426758, "start": 2, "stop": 73 } ], "seq_stats": { "molecular_weight": 11863.782799999984, "isoelectric_point": 10.197689628601072 }, "id": "KMADJIBOEG_908", "locus": "GCA000292445_00908" }, { "type": "cds", "sequence": "contig_1", "start": 496880, "stop": 497749, "strand": "-", "frame": 3, "gene": null, "product": "Serine hydroxymethyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2E8WS34" ], "nt": "ATGTTTTCAAAGGTAAGATTTTTACTTAATAAAGAGTTACAACGTCAAAGAGAAAACATTTGTTTAATCGCTTCAGAAAATTATGTTAGCCAAGACATATTAGCTGTAACTGGTTCAGTATTAACAAATAAATATGCAGAAGGCTATCCCAGTAAACGTTTTTATCGAGGCTGTGAAGTTGTTGATGAATCTGAAAACTTAGCCATTGAAAGTTGCAAAACTTTATTTGGAGCACAATGGGCTAATGTTCAACCTCATTCTGGATCATCTGCTAACTATGCAGTTTACTTAGCATTATTAAAACCAGGAGATACTATCTTAGGATTAGATCTTAATTGTGGTGGTCATTTAACCCATGGTAGCCCCGTTAATTTTTCAGGTAAGCAATATCAAGCAGTAACTTATTCGTTAGATTTTGAAACAGAAACTCTTGATTATGATGCAATTCTTCAAATTGCTCTCGAACACAAACCAAAGTTAATTATTTGTGGTTTTTCTAACTATTCTAGGACTGTTGACTTTAAAAAATTTAGTGCAATTGCAAAACAAGTTAATGCGTATCTTTTAGCTGATATTGCCCATATTGCTGGTTTCATCGCTGCAGGTTTGCACCAAAACCCTTTGCCTTTTGTGGATGTTGTCACTTCAACAACTCATAAAACTTTGCGTGGTCCTAGGGGGGGTATCATTATGTCTAACAACCAAGCAATTATCAAAAAGCTTGATAGTGGAGTATTTCCTGGATGTCAGGGTGGACCTTTACAACATGTGATAGCAGCTAAATATGTTTGTTTTAAAGAAGCTTTGAATCCAAAGTTTAAGCAGTATATGCAACAAGTTAAAGATAATGCTTTAGCAATGGCAAATTGA", "aa": "MFSKVRFLLNKELQRQRENICLIASENYVSQDILAVTGSVLTNKYAEGYPSKRFYRGCEVVDESENLAIESCKTLFGAQWANVQPHSGSSANYAVYLALLKPGDTILGLDLNCGGHLTHGSPVNFSGKQYQAVTYSLDFETETLDYDAILQIALEHKPKLIICGFSNYSRTVDFKKFSAIAKQVNAYLLADIAHIAGFIAAGLHQNPLPFVDVVTSTTHKTLRGPRGGIIMSNNQAIIKKLDSGVFPGCQGGPLQHVIAAKYVCFKEALNPKFKQYMQQVKDNALAMAN", "aa_hexdigest": "2d24ffa27521938064d283abd42d29fa", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0025FA9D98", "query_cov": 0.972318339100346, "subject_cov": 0.8593272171253823, "identity": 0.612, "score": 362.0, "evalue": 1.22e-122, "valid": false, "product": "serine hydroxymethyltransferase", "ec_ids": [ "2.1.2.1" ], "uniref50_id": "UniRef50_A0A2E8WS34", "kegg_orthology_id": "K00600" }, "pscc": { "uniref50_id": "UniRef50_A0A2E8WS34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2E8WS34" ], "product": "Serine hydroxymethyltransferase" }, "genes": [], "id": "KMADJIBOEG_909", "locus": "GCA000292445_00909" }, { "type": "cds", "sequence": "contig_1", "start": 497759, "stop": 498535, "strand": "-", "frame": 3, "gene": null, "product": "putative lipoprotein MG395", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47635", "UniRef:UniRef90_P47635" ], "nt": "TTGGGTGATAGTACTAATATATTTGAAACAAAAGATTTAGAACAACATTGGAACCATAGCTACTATCTTTTGGGTTATCCTGTACTTGAAAGAGGTTATGATCAAAACCGCTTATTAGAACAAAAAGAAGAGTTTACTAGAACTCATAACTTTAATCAAAAAAGTCAGTTATGGAGTAAAAACACATATTTAGTTTCAACTGCTAAAGAGATCCCTGTAATTACAAAAAATGCCAGAAAAGATGGTCAAATAGGTAGTGAAATATTTTCCAAAAAAACCCAAGACAGTCATGTTGATAAAGTTATTAAACACGAAAAAGGGATTGTTACATTTCAAAACTTCAAAAACTTTAAACTTAAATATCATGACAAAGAGTACCAACAGTATGGATATGGTTTAATGCTTGATGATACCAATCTCCCTGGTGGTTCTTCTGGAAGTGCTATATTTAACAACAACCAAAAAATTAACAGTATTTATTTCGGTGTTTTAGAGGTATATAAGAATTCCCAAACACATAAAGATAACATTGGTATGTCTCAACTTTTAAGAACTTCTAAACGTGAAAGTGAAAAAAATAAAACCAGAATTACTAATAATAGGGATAAATTTCAGCACTATGATTTAATTTTTGGTGACAGTAACACAAAATCTTTCTATGCACAATTTGCTAAAAAGCACAATACCCACTTGTACGACCAAATTAAAAATAGTGAAAAGGAAGAATTTAAATATGTTGATAAGAACAATCAAAAAACACCGTTTTTATTACGTTAG", "aa": "MGDSTNIFETKDLEQHWNHSYYLLGYPVLERGYDQNRLLEQKEEFTRTHNFNQKSQLWSKNTYLVSTAKEIPVITKNARKDGQIGSEIFSKKTQDSHVDKVIKHEKGIVTFQNFKNFKLKYHDKEYQQYGYGLMLDDTNLPGGSSGSAIFNNNQKINSIYFGVLEVYKNSQTHKDNIGMSQLLRTSKRESEKNKTRITNNRDKFQHYDLIFGDSNTKSFYAQFAKKHNTHLYDQIKNSEKEEFKYVDKNNQKTPFLLR", "aa_hexdigest": "6d259b0f8d10c58a74b30f019ef357d6", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 497759, "stop": 498835, "observations": { "mutation": [ 498586 ], "stop": [ 498586 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47635", "query_cov": 1.0, "subject_cov": 0.49236641221374045, "identity": 0.977, "score": 503.0, "evalue": 7.44e-176, "gene_start": 1, "gene_end": 258, "reference_start": 267, "reference_end": 524, "reference_sequence": "MLKIDMWFKLKSLGFSLISLQALLASCSAVSPVPVPIEEKNDSTTDNNATPFKDEQSDQGTEVNQQPKVEQKVYNRNFKFNTSFIPEESDIYRKGYDLTFTLNFTSFSNDSYGTGWLIDWKGDENTTQKQGSFFAYIATNLHVADGLRNIGDHWPYSKTDDQREFNEYESTVYFSIGKYTNKTDITKLYQEEKLEQRKVNDSLLSIQTSNIPKTAYTATNFLKGVNSIKPVYADFAVIELELNLENLRDWQIFNEFIKPAINTYKSLGDSTNIFETKDLEQHWNHSHYLLGYPVLERGYDQNRLLEQKEEFARTHNFNQKSQLWSKNTYLVSTAKEIPVITKNARKDGQIGSEIFSKKTQDSHVDKVIKHEKGIVTFQNFKNFKLKYHDKEYQQYGYGLMLDDTNLPGGSSGSAIFNNNQKINSIYFGVLEVYKNSKTHKDNIGMSQLLRTSKRESEKNKTRTTNNRDKFQHYDLIFGDSNTKSFYAQFAKKHNTHLYDQIKSSEKEEFKYVDKNNQKTPFLLR" }, "paralog": false, "identity": 0.9832402234636871, "score": 714.0, "evalue": 1.82e-261, "description": "Internal stop codon at 498586. Nonsense mutation around 498586.", "psc": { "uniref90_id": "UniRef90_P47635", "product": "Uncharacterized lipoprotein MG395", "uniref50_id": "UniRef50_P47635", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47635", "UniRef:UniRef50_P47635", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47635", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47635" ], "product": "Uncharacterized lipoprotein MG395" } }, "id": "KMADJIBOEG_910", "locus": "GCA000292445_00910" }, { "type": "cds", "sequence": "contig_1", "start": 498986, "stop": 499330, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAAGATTGATATGTGGTTTAAATTAAAAAGTTTAAGTTTTTCACTAATTTCGTTACAAGTACTTTTAGCTTCTTGTTCTGCTGTTTCACCTGTTCCTGTTCCTATAGAAAAAAACGACAGCACAACAGATAAAAACGCTACTCCTTTTAAAGATGAACAATCTGATCAAGGAACAGAAGTAAATCAGCAACCTAAGGTAGAACAGAAGGTTTATAACAGAAACTTTAAATTTAATACAAGTTTTATCCCAGAAGAAAGTGATATATACAGAAAAGGATATGATTTAACTTTTACTTTAAATTTCACTAGCTTTAGTAATGACTCTTATGGCACTGGTTGA", "aa": "MLKIDMWFKLKSLSFSLISLQVLLASCSAVSPVPVPIEKNDSTTDKNATPFKDEQSDQGTEVNQQPKVEQKVYNRNFKFNTSFIPEESDIYRKGYDLTFTLNFTSFSNDSYGTG", "aa_hexdigest": "f3651f5355946944cbdac19006844dee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12949.361499999986, "isoelectric_point": 5.03737850189209 }, "id": "KMADJIBOEG_911", "locus": "GCA000292445_00911" }, { "type": "cds", "sequence": "contig_1", "start": 499492, "stop": 499623, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACTAATGAAAGCAAATGCAACCATCAAAAAAGTTAAAAATGAAAATAGAACTACTATTGACTCAGGTAAACTAACTAAATTAAAACCAAAAAAATTAATCCCGTTAGAATTTGCATTGGCTTGA", "aa": "MKKLMKANATIKKVKNENRTTIDSGKLTKLKPKKLIPLEFALA", "aa_hexdigest": "fd1ad18d8bb42f3478c65d5b9d54ca67", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4853.9209, "isoelectric_point": 10.420943641662596 }, "id": "KMADJIBOEG_912", "locus": "GCA000292445_00912" }, { "type": "cds", "sequence": "contig_1", "start": 499702, "stop": 500160, "strand": "-", "frame": 1, "gene": "rpiB", "product": "ribose 5-phosphate isomerase B", "db_xrefs": [ "COG:COG0698", "COG:G", "EC:5.3.1.6", "GO:0004751", "GO:0009052", "GO:0019316", "RefSeq:WP_009885626.1", "SO:0001217", "UniParc:UPI000013973C", "UniRef:UniRef100_P47636", "UniRef:UniRef50_P47636", "UniRef:UniRef90_P47636" ], "nt": "ATGAGTTTTAATATCTTTATTGCTTCTGATCATACCGGTTTAACTCTTAAAAAAATTATTAGTGAACACCTAAAGACTAAGCAGTTTAATGTAGTTGATCTTGGCCCTAATTATTTTGATGCTAATGATGATTATCCTGACTTTGCTTTTTTGGTAGCTGATAAGGTCAAGAAAAATTCTGATAAAGATCTTGGGATACTAATTTGCGGTACTGGTGTTGGGGTTTGTATGGCAGCAAATAAAGTAAAAGGAGTATTAGCGGCTTTGGTTGTTAGTGAGAAAACAGCAGCTTTAGCTAGACAACATGATAATGCCAATGTTTTATGTCTTTCCAGTCGTTTTGTAACTGACAGTGAAAACATTAAAATAGTGGATGATTTTCTCAAAGCTAATTTTGAAGGTGGTAGACACCAAAGACGGATAGATAAGATTATTCGATATGAAAAAGAAACTGAATAG", "aa": "MSFNIFIASDHTGLTLKKIISEHLKTKQFNVVDLGPNYFDANDDYPDFAFLVADKVKKNSDKDLGILICGTGVGVCMAANKVKGVLAALVVSEKTAALARQHDNANVLCLSSRFVTDSENIKIVDDFLKANFEGGRHQRRIDKIIRYEKETE", "aa_hexdigest": "36c336623729202195316291c6d57640", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013973C", "ncbi_nrp_id": "WP_009885626.1", "uniref100_id": "UniRef100_P47636", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013973C", "RefSeq:WP_009885626.1", "UniRef:UniRef100_P47636" ] }, "ips": { "uniref100_id": "UniRef100_P47636", "uniref90_id": "UniRef90_P47636" }, "psc": { "uniref90_id": "UniRef90_P47636", "gene": "rpiB", "product": "ribose 5-phosphate isomerase B", "ec_ids": [ "5.3.1.6" ], "uniref50_id": "UniRef50_P47636", "cog_id": "COG0698", "cog_category": "G", "go_ids": [ "GO:0004751", "GO:0009052", "GO:0019316" ] }, "pscc": { "uniref50_id": "UniRef50_P47636", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47636" ], "product": "Probable ribose-5-phosphate isomerase B" }, "genes": [ "rpiB" ], "id": "KMADJIBOEG_913", "locus": "GCA000292445_00913" }, { "type": "cds", "sequence": "contig_1", "start": 500161, "stop": 500928, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MG397", "db_xrefs": [ "GO:0000921", "GO:0005940", "GO:0016020", "SO:0001217", "UniRef:UniRef50_P47637", "UniRef:UniRef90_P47637" ], "nt": "GTGGTTGAAAATATTGATGATTTTCGTAATCAAATTCAACAATATGAAAAGAAAAATGAGCTTTTAAAAACAACAATTAAAGCCATTGAATTAGTGTTCCAAAATGAAACAGATGTGCATGATTTAATTATTGAGCTTAATGAGAACTGCGATCTAATTACTAAAAATATTAATAACTTAAACCATCAAAGTTTAGCTCAGAATCAAATCAATTATGAAAAACTTTTTTACTTGATGAGTCAAACAAATCAAGCTTTAGAAAAACTGAAAAATTCACTAACTGATTTACTTAATTTAGCAGTTAATAAGTTTGATGACTATCGCTTTTTTGTCTATACTTTAGATGATTTCCGATTTAAATTTTTTCAGATTGAAAATTTAATTAGCGATCAAGAGTTAAAGGTTCCTGCTAAAACATTACAAGTAATTAATAAATCGAAATTAGAACTTGATAACATCTTCACTGAAGCTAATAATAACTATTTAGGTACTTTTAATTTGTTTAGTGAAAAGATAGATTTATTGCAAATTCAATTGACTGAAGTTATTTGTGATGTTGTTAGTTTAGTTAACTTAAGGCTAATGTGCAAACAAGCTTTTCTTTTTGCAAATAAATATCGTCAAAAGAGTAGTCATATCGATAGATCATTAGAACAGTTAACAAAACTATACAATCAACAAAACTATCCTGATACATTAGAAGGATTAATTAATCTTTTAACTAAAATTAAAACCAATACAAAACAAGACTATCTGGAACTGAATTAA", "aa": "MVENIDDFRNQIQQYEKKNELLKTTIKAIELVFQNETDVHDLIIELNENCDLITKNINNLNHQSLAQNQINYEKLFYLMSQTNQALEKLKNSLTDLLNLAVNKFDDYRFFVYTLDDFRFKFFQIENLISDQELKVPAKTLQVINKSKLELDNIFTEANNNYLGTFNLFSEKIDLLQIQLTEVICDVVSLVNLRLMCKQAFLFANKYRQKSSHIDRSLEQLTKLYNQQNYPDTLEGLINLLTKIKTNTKQDYLELN", "aa_hexdigest": "db71faae5af2d2713f6db5cbdfa50003", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 500161, "stop": 501228, "observations": { "mutation": [ 500973 ], "stop": [ 500973 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47637", "query_cov": 1.0, "subject_cov": 0.450530035335689, "identity": 0.988, "score": 485.0, "evalue": 2.56e-168, "gene_start": 1, "gene_end": 255, "reference_start": 312, "reference_end": 566, "reference_sequence": "MLVFLILLPFLLLVIFGAYLLVCLFVRQNNLLLKKTKQLYNNLKLSNFNCVISPFQLLRNEKQALEQSIKVLKQFQIKFDNEINSAFEQLTVYSKPNNIYRFIWFNRNIKLLNKNLQHLYTKQQEYINLTKNAIVYFNNSYDCLVFYRQAFCFLNQFINNFLIEKYDSLFYLNVLNRISLLFKEIENCIQAKDVEKQIAFLNKLHNILSQTIITANRQYMFDIKLSYLTHHFKVLIQKVKQMELMKNKVINSQDLLKLHELINNITIKLKDCTNFLNNLNLMLCERNMDEVQEAISWLFQAVSSKEKSINLVVENIDDFRNQIQQYEKKNELLKTTIKAIELVFQNETDVHDLIIELNENCDLITKNINNLNHQSLAQNQINYEKLFYLMSQTNQALEKLKNSLTDLLNLAVNKFDDYRFFVYTLDDFRFKFFQIENLISDQDLKVPAKTLQVINKSKLELDNIFTEANNNYLGTFNLFSEKIDLLQIQLTEVICDVVSLVNLRLMCKQAFLFANKYRQKSSHIDRSLEQLTKLYNRQNYPDTLEGLINLLTKIKTNTKQDYLELN" }, "paralog": false, "identity": 0.9830985915492958, "score": 686.0, "evalue": 9.32e-250, "description": "Internal stop codon at 500973. Nonsense mutation around 500973.", "psc": { "uniref90_id": "UniRef90_P47637", "product": "Uncharacterized protein MG397", "uniref50_id": "UniRef50_P47637", "go_ids": [ "GO:0016020", "GO:0005940", "GO:0000921" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47637", "UniRef:UniRef50_P47637", "GO:0016020", "GO:0005940", "GO:0000921" ] }, "pscc": { "uniref50_id": "UniRef50_P47637", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47637" ], "product": "Uncharacterized protein MG397" } }, "id": "KMADJIBOEG_914", "locus": "GCA000292445_00914" }, { "type": "cds", "sequence": "contig_1", "start": 500971, "stop": 501321, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAAAGAGATAGAAAATTGTATTCAAGCTAAAGATGTAGAAAAGCAAATTGCTTTTTTAAACAAATTACATAACATTCTCAGTCAAACAATTATTACTGCTAACAGACAATATATGTTTGATATTAAGTTAGGTTATTTAACCCATCACTTTAAGGTTTTAATTCAAAAGGTAAAGCAAATGGAATTAATGAAGAATAAAGTAATTAACAGTCAAGATCTCTTAAAAATTCATGAATTGATTAATAACATTACCATTAAATTAAAGGATTGTACCAATTTTCTCAATAACTTAAATTTAATGTTATGCGAAAGGAATATGGATGAAGTTCAAGAGGCGATCAGTTGA", "aa": "MFKEIENCIQAKDVEKQIAFLNKLHNILSQTIITANRQYMFDIKLGYLTHHFKVLIQKVKQMELMKNKVINSQDLLKIHELINNITIKLKDCTNFLNNLNLMLCERNMDEVQEAIS", "aa_hexdigest": "e832304b102e8f703259a9638a6006c1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13706.059999999985, "isoelectric_point": 8.439765739440919 }, "id": "KMADJIBOEG_915", "locus": "GCA000292445_00915" }, { "type": "cds", "sequence": "contig_1", "start": 501550, "stop": 501861, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGGTTTTTCTAATCTTGCTCCCTTTCTTACTACTAGTAATTTTTGGTGCTTATCTATTAGTTTGTCTTTTTGTTAGACAAAATAACTTACTTTTAAAAAAAACCAAACAACTCTATAATAACTTGAAATTAAGCAATTTCAATTGTGTGATTAGTCCATTTCAATTACTAAGAAATGAAAAACAAGCGTTAGAACAATCCATTAAAGTTTTAAAACAGTTTCAAATCAAATTTGATAATGAAATCAATTCAGCTTTTGAGCAACTAACAGTTTACAGTAAACCAAATAATATTTATCGTTTCATCTGA", "aa": "MLVFLILLPFLLLVIFGAYLLVCLFVRQNNLLLKKTKQLYNNLKLSNFNCVISPFQLLRNEKQALEQSIKVLKQFQIKFDNEINSAFEQLTVYSKPNNIYRFI", "aa_hexdigest": "8ed337e99d3e8149333b4e559996cda4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12188.453599999986, "isoelectric_point": 9.697221565246583 }, "id": "KMADJIBOEG_916", "locus": "GCA000292445_00916" }, { "type": "cds", "sequence": "contig_1", "start": 501863, "stop": 502264, "strand": "-", "frame": 3, "gene": "atpC", "product": "F0F1 ATP synthase subunit epsilon", "db_xrefs": [ "COG:C", "COG:COG0355", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0045261", "GO:0046933", "RefSeq:WP_014894320.1", "SO:0001217", "UniParc:UPI00027B42B6", "UniRef:UniRef100_UPI00027B42B6", "UniRef:UniRef50_P47638", "UniRef:UniRef90_P47638" ], "nt": "ATGAAGTTATTGCACTTTTTGGTACTTAGTCCTAGTGGCATAAAACTAGATAAAACCATTATTAGTGCGCAAGTTAAAACTACTGAAGGTTACATAGGATTAAATTTTAATCGCGCTCCTTTGATTGCTGCTATTCAATCCCATCTGTGCAAAATTATTTTTGCTGATCAAACAAAAAGAGAAGCAATTATTGGTGCTGGTTTAATGCTTATTAAAAAAACAGAAGCTAAGATTTTCACAGAAAATTTTGTTTTTGCTGATGAAGTTGATATTAATGAAACCTTAAAAAGAAAAACAGAACTTGAAAGAAAAATTTATCATATCAATGATGCTAAGCTAAACGTTAAAATTGAACAAAATTTAATGTTTGAATTATTAAAACTTTCAAGTAAGAAAAAATAA", "aa": "MKLLHFLVLSPSGIKLDKTIISAQVKTTEGYIGLNFNRAPLIAAIQSHLCKIIFADQTKREAIIGAGLMLIKKTEAKIFTENFVFADEVDINETLKRKTELERKIYHINDAKLNVKIEQNLMFELLKLSSKKK", "aa_hexdigest": "76addca61e396ef877938dbcaf5f5ff5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B42B6", "ncbi_nrp_id": "WP_014894320.1", "uniref100_id": "UniRef100_UPI00027B42B6", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B42B6", "RefSeq:WP_014894320.1", "UniRef:UniRef100_UPI00027B42B6" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B42B6", "uniref90_id": "UniRef90_P47638" }, "psc": { "uniref90_id": "UniRef90_P47638", "gene": "atpC", "product": "F0F1 ATP synthase subunit epsilon", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47638", "cog_id": "COG0355", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0045261", "GO:0005524", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P47638", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47638" ], "product": "ATP synthase epsilon chain" }, "genes": [ "atpC" ], "id": "KMADJIBOEG_917", "locus": "GCA000292445_00917" }, { "type": "cds", "sequence": "contig_1", "start": 502269, "stop": 503699, "strand": "-", "frame": 2, "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "db_xrefs": [ "COG:C", "COG:COG0055", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0016887", "GO:0045261", "GO:0046933", "GO:0046961", "SO:0001217", "UniRef:UniRef50_P50002", "UniRef:UniRef90_Q50331" ], "nt": "TTGATAAAAAAAGAAAACCTAACATATGGTAAAGTTCACCAAGTCATTGGTTCTGTAGTTGATGTTATCTTTTCAGAAAGTAAACAATTACCTAGAGTTTATGATTGTTTGAGTGTACAACTAGAAAAAAGTGAGCTTTTTTTAGAAGCAACCCAATTAATAGGTGATGACATTGTTCGTTGCATTGCATTAGGTCCTACAGAAGGATTAGCACGTAATGTTAAAGTTACTAACTATAACCATCCAATAGAGGTACCTGTTGGCAAAAATGTATTGGGAAGGATGTTCAATGTTTTAGGTGAACCCATTGATGGAAAAGAACCATTACCAAAAAAACCAAAGCTATCAATCCATCGTAACCCACCTACTTTTGATGAACAACCAAATACTGTTGATATTTTTGAAACAGGAATAAAAGTAATTGATCTTTTAACTCCTTACGTTAGGGGGGGTAAAATTGGTTTATTTGGAGGAGCTGGTGTTGGGAAAACTGTTTTGGTGCAAGAATTAATTCATAACATTGCCAAAGAACATTCTGGTTTAAGTGTATTTGCTGGAGTTGGTGAAAGAACAAGAGAAGGTAATGATCTTTACTATGAAATGATTCAAGGTGGGGTGATTGATAAAACAGTTTTAGTTTTTGGCCAAATGAATGAACCACCAGGAGCTAGAATGAGAGTTGCTTTAACTGCTTTAACAATGGCAGAATATTTTCGTGATCATGATAATCAGAATGTGCTGTTATTCATTGACAATATTTTTCGTTTTACTCAAGCAGGTAGTGAGGTTTCAGCATTACTTGGTAGAATGCCATCTGCTGTTGGCTATCAACCAACTTTAGCTATTGAAATGGGTAAGTTACAAGAAAGAATTGCTTCTACCAAAACAGGTTCTATTACATCTGTTCAAGCTATCTATGTTCCAGCAGATGATCTAACAGACCCAGCACCTGCAACAACATTTACCCATCTTGATGCTAAAACAGTGTTGGATCGTAATATTGCAGCACTAGGTATTTTTCCAGCAATTAATCCTTTAGAATCAACAAGTCGTTTATTAGATCCTAGTGTTGTTGGTATCAACCATTATAAAGTCGCTTTAGGAGTGCAAAATATCTTGCAGCGTTTTGCAGAATTACAAGATATCATTGCCATACTAGGAATTGATGAATTGTCTGATGAAGATAAGATTATTGTTGAAAGAGCAAGAAGGATACGTAACTTTTTATCCCAACCTTTTTTTGTTGCTGAAAAGTTTTCAGGTATTGCAGGTAAATATGTATCTTTAAATGACACTGTTCAATCTTTTAAAGAAATTTTGGAAGGTAAGCATGATCATTTGCCTGAACAAGCATTCTTTTATGTTGGAACCATTCAAGAGGCTGTTGAAAAAGCAAAAAGATTAAATCAAGAGTTTGATAAAACTAAGTAG", "aa": "MIKKENLTYGKVHQVIGSVVDVIFSESKQLPRVYDCLSVQLEKSELFLEATQLIGDDIVRCIALGPTEGLARNVKVTNYNHPIEVPVGKNVLGRMFNVLGEPIDGKEPLPKKPKLSIHRNPPTFDEQPNTVDIFETGIKVIDLLTPYVRGGKIGLFGGAGVGKTVLVQELIHNIAKEHSGLSVFAGVGERTREGNDLYYEMIQGGVIDKTVLVFGQMNEPPGARMRVALTALTMAEYFRDHDNQNVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLAIEMGKLQERIASTKTGSITSVQAIYVPADDLTDPAPATTFTHLDAKTVLDRNIAALGIFPAINPLESTSRLLDPSVVGINHYKVALGVQNILQRFAELQDIIAILGIDELSDEDKIIVERARRIRNFLSQPFFVAEKFSGIAGKYVSLNDTVQSFKEILEGKHDHLPEQAFFYVGTIQEAVEKAKRLNQEFDKTK", "aa_hexdigest": "66bd13b40b7068befd757585f03a606b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q50331", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.9940000000000001, "score": 926.0, "evalue": 0.0, "valid": true, "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P50002", "cog_id": "COG0055", "cog_category": "C", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0016887", "GO:0045261", "GO:0046933", "GO:0046961" ] }, "pscc": { "uniref50_id": "UniRef50_P50002", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P50002" ], "product": "ATP synthase subunit beta, sodium ion specific" }, "genes": [ "atpD" ], "id": "KMADJIBOEG_918", "locus": "GCA000292445_00918" }, { "type": "cds", "sequence": "contig_1", "start": 503701, "stop": 504096, "strand": "-", "frame": 1, "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "db_xrefs": [ "COG:C", "COG:COG0224", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933", "SO:0001217", "UniRef:UniRef50_P47640", "UniRef:UniRef90_P47640" ], "nt": "ATGGATGCATTTAAAGAGTTTAAACTTGATCGAATTTGTATGGTTTACACTAAATTTAAAAACTCATTAATCCAACAATCTCAGCTCTTTCAAGTTTTTCCTTTCGATGTTGAAACTTTTAAAACTTTAAATCCGGTTGTAACTGATCAACAACTTGATTTTGAGCCAGATCAAGCCACGATAATTAATTTAATTACTCCACAGTTTTTTGATGTGGCTCTGTATGGTGGCCTTGTTGAAACTAAGTTATGTGAATCAGCTTCTAGACAAAATGCAATGGAAGCTGCTACAAAGAATGCTAAAGATTTACTTGATAAATACACTTTACAATTTAACAAGCTAAGACAAAACTCTATTACAGAAGAGATTATTGAAGTTATAGGAGGTATGAATTAA", "aa": "MDAFKEFKLDRICMVYTKFKNSLIQQSQLFQVFPFDVETFKTLNPVVTDQQLDFEPDQATIINLITPQFFDVALYGGLVETKLCESASRQNAMEAATKNAKDLLDKYTLQFNKLRQNSITEEIIEVIGGMN", "aa_hexdigest": "b53748a57bc025a0916f30df669a8818", "start_type": "ATG", "rbs_motif": null, "genes": [ "atpG" ], "truncated": "3-prime", "pseudogene": { "start": 503701, "stop": 504394, "observations": { "deletion": [ 504220, 504349 ], "stop": [ 504193 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47640", "query_cov": 1.0, "subject_cov": 0.46953405017921146, "identity": 1.0, "score": 259.0, "evalue": 1.23e-85, "gene_start": 1, "gene_end": 131, "reference_start": 149, "reference_end": 279, "reference_sequence": "MAFIQEIKRRMNTVKSTIKITNAMKMVSRAKFIKFKKQFQEISLFFNEFYKAVGQVVVSLKEPKKKPDNQKTLWIMMSSSLGLCGQHNSNMNKLLKANFKADDKIFFLGRKNQSFWNKNSQYNPAVGFIDIQDRDINFDYCQTIFDQIMDAFKEFKLDRICMVYTKFKNSLIQQSQLFQVFPFDVETFKTLNPVVTDQQLDFEPDQATIINLITPQFFDVALYGGLVETKLCESASRQNAMEAATKNAKDLLDKYTLQFNKLRQNSITEEIIEVIGGMN" }, "paralog": false, "identity": 0.9956709956709957, "score": 450.0, "evalue": 4.25e-163, "description": "Internal stop codon at 504193. Frameshift due to deletion around 504220, 504349.", "psc": { "uniref90_id": "UniRef90_P47640", "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47640", "cog_id": "COG0224", "cog_category": "C", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47640", "EC:7.1.2.2", "UniRef:UniRef50_P47640", "COG:COG0224", "COG:C", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P47640", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47640" ], "product": "ATP synthase gamma chain" } }, "id": "KMADJIBOEG_919", "locus": "GCA000292445_00919" }, { "type": "cds", "sequence": "contig_1", "start": 504216, "stop": 504314, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGAGTTCTTCTTTAGGACTTTGTGGACAGCATAATTCGAACATGAATAAGTTATTAAAAGCTAATTTTAAAGCTGATGATAAAATCTTTTTTTAG", "aa": "MMSSSLGLCGQHNSNMNKLLKANFKADDKIFF", "aa_hexdigest": "c2be6ae5ea05735ad1db5f8d94257d03", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3591.1661000000004, "isoelectric_point": 9.102694129943849 }, "id": "KMADJIBOEG_920", "locus": "GCA000292445_00920" }, { "type": "cds", "sequence": "contig_1", "start": 504314, "stop": 504538, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00231.24" ], "nt": "ATGGCTTTTATACAAGAAATTAAGCGCAGAATGAATACAGTAAAATCCACCATTAAGATAACTAATGCAATGAAAATGGTGTCACGCGCTAAGTTTATTAAGTTCAAAAAACAGTTTCAAGAAATTAGTTTGTTTTTTAATGAATTTTATAAAGCTGTTGGCCAAGTAGTTGTTTCTTTAAAAGAACCAAAAAGAAACCAGATAACCAAAAAACTTTATGGATAA", "aa": "MAFIQEIKRRMNTVKSTIKITNAMKMVSRAKFIKFKKQFQEISLFFNEFYKAVGQVVVSLKEPKRNQITKKLYG", "aa_hexdigest": "4846c2f11dbedf2758d4388d21e8c040", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00231.24", "name": "ATP-synt", "length": 59, "aa_cov": 0.7972972972972973, "hmm_cov": 1.0, "evalue": 2.4573323391680667e-14, "score": 48.78495788574219, "start": 4, "stop": 62 } ], "seq_stats": { "molecular_weight": 8778.473599999994, "isoelectric_point": 10.576247978210446 }, "id": "KMADJIBOEG_921", "locus": "GCA000292445_00921" }, { "type": "cds", "sequence": "contig_1", "start": 504538, "stop": 505326, "strand": "-", "frame": 1, "gene": null, "product": "ATP synthase alpha chain", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q1RKD9" ], "nt": "TTGATTGTATTTGATGACCTTTCTAAGCATGCTGTTGCTTACAGAACTCTTTCACTCTTGTTAAAAAGACCACCTGGTAGAGAAGCTTTTCCAGGAGATGTTTTTTATTTACATTCAAGACTTTTGGAACGTGCATGCAAGTTAAATGATGAAAATGGTGGTGGCTCAATTACAGCTTTACCAATTATAGAAACTCAAGCTGGTGATATCTCTGCATATATTCCTACAAATGTTATTTCAATTACTGATGGCCAACTGTTTATGGTTAGTAGTCTATTTAACGCTGGACAACGCCCTGCAATTCAAATTGGTTTATCAGTTTCAAGGGTTGGTAGTGCAGCACAAACAAAAGCGATTAAACAGCAAACTGGCAGTTTAAAACTAGAACTTGCTCAGTATAGTGAACTTGATAGTTTTAGTCAATTTGGTAGTGATCTTGATGAAAATACAAAAAAGGTTTTAGAGCATGGTAAAAGAGTAATGGAAATGATTAAACAACCAAATGGTAAACCTTACTCTCAAGTCCATGAAGCATTATTTTTATTTGCTATTAACAAAGCTTTCATTAAGTTTATTCCAGTTGATGAAATTGCTAAATTTAAACAAAGGATAACAGAAGAATTTAATGGTTCCCATCCTCTGTTTAAAGAGTTATCTAACAAAAAAGAATTTACTGAGGATTTAGAAAGTAAAACTAAAACCGCTTTTAAAATGCTTGTGAAACGTTTTATCAGTACATTAACAGATTATGATATTACCAAATTTGGTAGTATTGAGGAACTTAATTAA", "aa": "MIVFDDLSKHAVAYRTLSLLLKRPPGREAFPGDVFYLHSRLLERACKLNDENGGGSITALPIIETQAGDISAYIPTNVISITDGQLFMVSSLFNAGQRPAIQIGLSVSRVGSAAQTKAIKQQTGSLKLELAQYSELDSFSQFGSDLDENTKKVLEHGKRVMEMIKQPNGKPYSQVHEALFLFAINKAFIKFIPVDEIAKFKQRITEEFNGSHPLFKELSNKKEFTEDLESKTKTAFKMLVKRFISTLTDYDITKFGSIEELN", "aa_hexdigest": "8f4965ef61aad525a43c35b53ea2ecc4", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q1RKD9", "query_cov": 0.9351145038167938, "subject_cov": 0.8419243986254296, "identity": 0.58, "score": 279.0, "evalue": 7.85e-91, "valid": false, "gene": "atpA", "product": "F0F1 ATP synthase subunit alpha", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q1RKD9", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_Q1RKD9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q1RKD9" ], "product": "ATP synthase alpha chain" }, "genes": [], "id": "KMADJIBOEG_922", "locus": "GCA000292445_00922" }, { "type": "cds", "sequence": "contig_1", "start": 505348, "stop": 506094, "strand": "-", "frame": 1, "gene": null, "product": "ATP synthase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9E1" ], "nt": "ATGGCAGATAAACTAAATGAATACGTAGCATTAATCAAAACTGAAATTAAAAAGTATTCCAAAAAAATATTTAACAGTGAAATTGGTCAAGTCATTAGTGTTGCTGATGGAATTGCCAAGGTTAGTGGACTTGAAAATGCTTTATTAAATGAGTTAATTCAATTTGAAAATAATATTCAAGGAATAGTATTAAACCTTGAACAAAATACAGTCGGAATAGCACTTTTTGGTGACTATTCTTCGTTACGAGAAGGCAGTACCGCTAAAAGAACCCACAGTGTAATGAAAACTCCTGTTGGTGATGTTATGCTTGGTAGAATCGTCAATGCACTTGGTGAAGCAATTGATGGTAGAGGTGATATTAAAGCTACTGAATATGATCAAATAGAAAAAATTGCTCCAGGTGTAATGAAAAGGAAAAGTGTTAACCAACCACTTGAAACTGGAATCTTAACAATTGATGCTTTATTTCCTATAGGTAAAGGACAACGTGAATTAATTGTTGGTGATAGACAAACAGGTAAAACTGCTATTGCGATTGACACTATCATTAATCAAAAAGATAAAGATGTTTATTGTGTTTATGTAGCAATTGGTCAAAAAAATTCATCAGTAGCACAAATTGTACACCAACTTGAAGTTAATGATTCAATGAAATACACTACAGTGGTTTGTGCTACAGCTAGTGACTCTGATTCCATGGTTTATTTAAGTCCTTTTACAGGAATAACTATTGCTGAATATTGA", "aa": "MADKLNEYVALIKTEIKKYSKKIFNSEIGQVISVADGIAKVSGLENALLNELIQFENNIQGIVLNLEQNTVGIALFGDYSSLREGSTAKRTHSVMKTPVGDVMLGRIVNALGEAIDGRGDIKATEYDQIEKIAPGVMKRKSVNQPLETGILTIDALFPIGKGQRELIVGDRQTGKTAIAIDTIINQKDKDVYCVYVAIGQKNSSVAQIVHQLEVNDSMKYTTVVCATASDSDSMVYLSPFTGITIAEY", "aa_hexdigest": "f82f3fb9500a29f04daa791e2578ff9f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A223M9E1", "query_cov": 0.9637096774193549, "subject_cov": 0.9372549019607843, "identity": 0.565, "score": 264.0, "evalue": 1.17e-85, "valid": false, "product": "ATP synthase subunit alpha", "uniref50_id": "UniRef50_A0A223M9E1" }, "pscc": { "uniref50_id": "UniRef50_A0A223M9E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9E1" ], "product": "ATP synthase subunit alpha" }, "genes": [], "id": "KMADJIBOEG_923", "locus": "GCA000292445_00923" }, { "type": "cds", "sequence": "contig_1", "start": 506110, "stop": 506640, "strand": "-", "frame": 1, "gene": "atpH", "product": "ATP synthase F1 subunit delta", "db_xrefs": [ "COG:C", "COG:COG0712", "EC:7.1.2.2", "GO:0005886", "GO:0015986", "GO:0045261", "GO:0046933", "RefSeq:WP_009885621.1", "SO:0001217", "UniParc:UPI00001264A9", "UniRef:UniRef100_P47642", "UniRef:UniRef50_P47642", "UniRef:UniRef90_P47642" ], "nt": "ATGATTAATGCACAAGCATTTGGAACTGCACTTTTTCAATTAAGTGAAGAGCAAAAACAAGTAAAGAAAATTTATGAAGAGTGCCATTTTTTTCTGAAATTAATGCGTAATTTTAAAGATGGTTCATTATCGTTCTTACTTAATTCTTATACACTAACAAAACCAGATAAAATAAGACTTGTTGATAAGTTGTTTAAAAATCATTTTTGTCAAGTTTTTGTTGATTTTTTAAAAGTAATTATTTTAAAGGGTTACTTTACTTTAGTTGAACAGGCAATTAAGTATTTTTTTGATAATGTTGAAAGTCAAAAACACATTCAATTTATCAAAATAATTACTGCTTTTGAATTAAGCTCAAAACAACTTAACAAAATTATTGCAATAATGGAAAAACGTTTTAAAACAAAGGTTGTTTATAAAACTGAGATTGATCGCAGTTTAATTTCAGGAATTAGGATAGAATCAAGTTCCCATTTATTTGAAAAAAATGTGCGTGATGAATTAAAACGCATAATGGCCCATTTTATTTAA", "aa": "MINAQAFGTALFQLSEEQKQVKKIYEECHFFLKLMRNFKDGSLSFLLNSYTLTKPDKIRLVDKLFKNHFCQVFVDFLKVIILKGYFTLVEQAIKYFFDNVESQKHIQFIKIITAFELSSKQLNKIIAIMEKRFKTKVVYKTEIDRSLISGIRIESSSHLFEKNVRDELKRIMAHFI", "aa_hexdigest": "24fcfa4384aa9db8bfb4f51d519b298b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001264A9", "ncbi_nrp_id": "WP_009885621.1", "uniref100_id": "UniRef100_P47642", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001264A9", "RefSeq:WP_009885621.1", "UniRef:UniRef100_P47642" ] }, "ips": { "uniref100_id": "UniRef100_P47642", "uniref90_id": "UniRef90_P47642" }, "psc": { "uniref90_id": "UniRef90_P47642", "gene": "atpH", "product": "ATP synthase F1 subunit delta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47642", "cog_id": "COG0712", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P47642", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47642" ], "product": "ATP synthase subunit delta" }, "genes": [ "atpH" ], "id": "KMADJIBOEG_924", "locus": "GCA000292445_00924" }, { "type": "cds", "sequence": "contig_1", "start": 506633, "stop": 506878, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAATTAAAAAGTGAAATAGAAAAAACAGCAAATCGCCAAGCTAACTTAATGATTTTTCAAGCTCGTCAGGAAATTGAAAAAGAAAGACGTTCTCTTAAAGAACAATCTATTAAAGAGAGTGTGGAATTGGCTATGTTGGCTGCACAAGAACTAATTCTCAAGAAAATAGATCAAAAATCAGATAGAGAATTTATTGATAAGTTTATTAGAGATTTAGAAGCTAACGAAACAGAAGATGATTAA", "aa": "MQLKSEIEKTANRQANLMIFQARQEIEKERRSLKEQSIKESVELAMLAAQELILKKIDQKSDREFIDKFIRDLEANETEDD", "aa_hexdigest": "a8c108098cbf6bdcdc66eb92a6854592", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9596.800399999991, "isoelectric_point": 4.959622764587402 }, "id": "KMADJIBOEG_925", "locus": "GCA000292445_00925" }, { "type": "cds", "sequence": "contig_1", "start": 507262, "stop": 507570, "strand": "-", "frame": 1, "gene": "atpE", "product": "F0F1 ATP synthase subunit C", "db_xrefs": [ "COG:C", "COG:COG0636", "EC:7.1.2.2", "GO:0005886", "GO:0008289", "GO:0015986", "GO:0045263", "GO:0046933", "RefSeq:WP_009885619.1", "SO:0001217", "UniParc:UPI00001265D0", "UniRef:UniRef100_P47644", "UniRef:UniRef50_P47644", "UniRef:UniRef90_P47644" ], "nt": "ATGGAACATGTTAATGAAATTTTAGCTACAGTTGGTGTTATATTACAACAAACTCAAACTACCCAGGATGTTAACGCTAGTGCTAAGCTAGGTGCTTATATAGGTGCTGGTGTTACTATGATTGCAGGTTCAACTGTAGGGATTGGACAAGGTTATATTTTTGGTAAAGCTGTTGAGGCAATAGCAAGAAATCCTGAAGTTGAAAAACAGGTTTTTAAACTAATTTTCATTGGTTCTGCTGTTTCTGAATCTACAGCAATTTATGGACTTTTAATTTCCTTTATCTTAATTTTTGTAGCAGGAGCTTAA", "aa": "MEHVNEILATVGVILQQTQTTQDVNASAKLGAYIGAGVTMIAGSTVGIGQGYIFGKAVEAIARNPEVEKQVFKLIFIGSAVSESTAIYGLLISFILIFVAGA", "aa_hexdigest": "a5fc1b6dc3117211faa233e9c535be74", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001265D0", "ncbi_nrp_id": "WP_009885619.1", "uniref100_id": "UniRef100_P47644", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001265D0", "RefSeq:WP_009885619.1", "UniRef:UniRef100_P47644" ] }, "ips": { "uniref100_id": "UniRef100_P47644", "uniref90_id": "UniRef90_P47644" }, "psc": { "uniref90_id": "UniRef90_P47644", "gene": "atpE", "product": "F0F1 ATP synthase subunit C", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47644", "cog_id": "COG0636", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0045263", "GO:0008289", "GO:0046933", "GO:0015986" ] }, "pscc": { "uniref50_id": "UniRef50_P47644", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47644" ], "product": "ATP synthase subunit c" }, "genes": [ "atpE" ], "id": "KMADJIBOEG_926", "locus": "GCA000292445_00926" }, { "type": "cds", "sequence": "contig_1", "start": 507573, "stop": 507680, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGACTTATAATTATTGGGCTAAAATGCGCAATCAAGGTTTGGAAAATAATAATGCAAGTGAATTACACTTTAAAGGCATAAAGGTAATTCAAGAAAATATTTAG", "aa": "MLTYNYWAKMRNQGLENNNASELHFKGIKVIQENI", "aa_hexdigest": "e21ecaf31b276c6f44f31206fb2bcdd5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4168.7115, "isoelectric_point": 8.161520195007324 }, "id": "KMADJIBOEG_927", "locus": "GCA000292445_00927" }, { "type": "cds", "sequence": "contig_1", "start": 507727, "stop": 507828, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGTAATACTGGGGTTATTAAACCTGCAAAAACTGTTCCTAAGCTAAGTGCTAATGGTTGGTTATTAATACTTGAAAAAATAAAAATTCAAAAGTTATAA", "aa": "MCNTGVIKPAKTVPKLSANGWLLILEKIKIQKL", "aa_hexdigest": "59765f25d69fbbb03ccc30b587ed89f7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3649.5006000000003, "isoelectric_point": 10.025236701965333 }, "id": "KMADJIBOEG_928", "locus": "GCA000292445_00928" }, { "type": "cds", "sequence": "contig_1", "start": 507859, "stop": 508149, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTCCCCATAACCTTAATGAAATAGAAAAAAGCGGTGCAAATCCACTCAATATACTAAAAGGATTTGGAATGAAAGTAGAATACTTTTTTCCTTTAACAGTAATTCCAAAGGCAAACTCTTTAAAAAAATTTCATCTTTGGTATCTAATCCCCATAACAACAATCCCAATAAAAGTTGCAAGTCCTAAAGAAAAAGTAAATGTTAAAGATGATGTTGGTGGTGAAATACCACCAAGCAAGCTAACTAAGTTGCTTGATACTATGTACAGAAGCAACATTAAAAAGTAG", "aa": "MFPHNLNEIEKSGANPLNILKGFGMKVEYFFPLTVIPKANSLKKFHLWYLIPITTIPIKVASPKEKVNVKDDVGGEIPPSKLTKLLDTMYRSNIKK", "aa_hexdigest": "52e48c4e32d4aa890d5b2d0a5b8d4c0d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10895.842099999989, "isoelectric_point": 9.73641834259033 }, "id": "KMADJIBOEG_929", "locus": "GCA000292445_00929" }, { "type": "cds", "sequence": "contig_1", "start": 508441, "stop": 508620, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCGACATGTTTTCTTTTCGATTTAGATATTAAATTGATAAAAAAATATTCTAAAGGTAACCAAACAAATGATAACACTAAATTTAGTATTGGAAAAAACAATGTTGAAATTATTGTTGCAATAGTGTTAAAAATTGACGGAGTGACAACAAAAGAAATAATCACAGGAATTAAATAA", "aa": "MATCFLFDLDIKLIKKYSKGNQTNDNTKFSIGKNNVEIIVAIVLKIDGVTTKEIITGIK", "aa_hexdigest": "10eba45f6b21e8374251f8065f309968", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6559.688700000001, "isoelectric_point": 9.20764865875244 }, "id": "KMADJIBOEG_930", "locus": "GCA000292445_00930" }, { "type": "cds", "sequence": "contig_1", "start": 508636, "stop": 508908, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAAAAAATGCAAAGAATGAAACTGCTTTTTGCTTTTTGACATTTTTCAAACTTGCAAAGAAAGATGCTAATCTTAAAACTGATAGTGAATTTAAAACAGCAAAAGGTAATGGCAACATTCAACCAAATAAAATGTCCAAATTATTGCCTTTTGTTAAAACAACAACTGCTATTAAAATTAGTACAAATAATCAAGCTACAAAAATAATTAAGGTAATAATTTTTGCAACCTTTCTATTGTTTCAATTGAACAAAACTTTTAGATGTTAA", "aa": "MKKNAKNETAFCFLTFFKLAKKDANLKTDSEFKTAKGNGNIQPNKMSKLLPFVKTTTAIKISTNNQATKIIKVIIFATFLLFQLNKTFRC", "aa_hexdigest": "ade6e1ebbd4216ea000fa20191d633df", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10211.04439999999, "isoelectric_point": 10.232824897766111 }, "id": "KMADJIBOEG_931", "locus": "GCA000292445_00931" }, { "type": "cds", "sequence": "contig_1", "start": 508940, "stop": 509398, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00113.28", "PFAM:PF07476.17", "PFAM:PF13378.12" ], "nt": "ATGATTGCTTACTTAGAAAAATTAACAAAAAAATATCCAATTATTTCAATAGAAGATGGTTTGAGTGAAAATGATTGGGAAGGGATGAACCAATTAACTAAAACCATAGGTAGCCATATTCAAATTGTTGGTGATGACACTTACTGTACTAATGCAGAACTTGCTAAAAAAGGTGTTGCACAAAATACAACAAACTCGATATTGATTAAATTAAATCAAATTGGTTCTATTAGTGAAACGATTCAAACAATTGAAGTTGCAAAAAAAGCTAACTGGAGTCAAGTAATTTCACATCGCAGTGGTGAAACAGAAGATACAACTATTGCTGATTTGGCAGTTGCTGCCCAAACTGGTCAAATTAAAACCGGTTCAATGTCACGCTCAGAAAGAATAGCTAAATACAATCGTTTGTTGTACATAGAAATTGAACTTGGTGATAAAGGAAAATACTTAGGTTGA", "aa": "MIAYLEKLTKKYPIISIEDGLSENDWEGMNQLTKTIGSHIQIVGDDTYCTNAELAKKGVAQNTTNSILIKLNQIGSISETIQTIEVAKKANWSQVISHRSGETEDTTIADLAVAAQTGQIKTGSMSRSERIAKYNRLLYIEIELGDKGKYLG", "aa_hexdigest": "686449392d6dd3075657c0340eaef22d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2G9XGH8", "query_cov": 1.0, "subject_cov": 0.8351648351648352, "identity": 0.599, "score": 187.0, "evalue": 6.68e-58, "valid": false, "product": "Enolase", "ec_ids": [ "4.2.1.11" ], "uniref50_id": "UniRef50_A0A350MM12", "kegg_orthology_id": "K01689" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00113.28", "name": "Enolase_C", "length": 151, "aa_cov": 0.993421052631579, "hmm_cov": 1.0, "evalue": 1.5013108740720047e-66, "score": 219.78880310058594, "start": 2, "stop": 152 }, { "id": "PF07476.17", "name": "MAAL_C", "length": 112, "aa_cov": 0.6776315789473685, "hmm_cov": 0.9910714285714286, "evalue": 7.072625677067437e-08, "score": 27.034103393554688, "start": 17, "stop": 119 }, { "id": "PF13378.12", "name": "MR_MLE_C", "length": 127, "aa_cov": 0.8289473684210527, "hmm_cov": 0.952755905511811, "evalue": 1.614185485239068e-08, "score": 29.636306762695312, "start": 4, "stop": 129 } ], "seq_stats": { "molecular_weight": 16751.811199999996, "isoelectric_point": 5.7214357376098635 }, "id": "KMADJIBOEG_932", "locus": "GCA000292445_00932" }, { "type": "cds", "sequence": "contig_1", "start": 509531, "stop": 510274, "strand": "-", "frame": 3, "gene": null, "product": "Enolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E2U821" ], "nt": "ATGGGAAGTTCAAATCTAAACATCAATTCAAAAATAACCGATATTTTTGCTTATCAAGTTTTTGATTCTCGGGGTGTTCCAACAGTAGCTTGTGTTGTTAAATTGGCATCTGGTCATGTAGGTGAAGCGATGGTTCCATCAGGTGCTTCTACAGGTGAGAAAGAAGCAATTGAATTACGTGATAATGATCCAAAAAATTATTTTGGTAAAGGCGTTAACAAAGCCGTTGATAACGTTAATAAAGTTATTGCCCCTAAGCTTATTGGCTTAAATGCATTTGATCAATTAACAGTGGATCAAGCAATGATTAAACTAGACAATACTCCCAACAAAGCAAAATTAGGAGCAAATGCTATATTATCTGTTTCACTTGCAGTATCAAAAGCAGCAGCAAAAGCACAAAACAGCTCATTATTTCAATACATTTCAAATAAATTAATTGGATTAAATACAACAAATTTTGTTTTACCTGTGCCAATGTTAAATGTAATTAATGGTGGTGCTCATGCTGATAACTATATTGATTTTCAAGAGTTCATGATCATGCCTTTAGGTGCTAAAAAGATGCATGAAGCTTTAAAAATGGCTAGTGAAACTTTTCATGCTTTACAAAATCTTTTAAAAAAGCGTGGATTAAACACAAATAAAGGAGATGAAGGTGGATTTGCGCCTAACTTAAAACTTGCAGAAGATGCACTTGACATCATGGTTGAAGCCATTAAATTAGCTGGATATAAGCCTTGA", "aa": "MGSSNLNINSKITDIFAYQVFDSRGVPTVACVVKLASGHVGEAMVPSGASTGEKEAIELRDNDPKNYFGKGVNKAVDNVNKVIAPKLIGLNAFDQLTVDQAMIKLDNTPNKAKLGANAILSVSLAVSKAAAKAQNSSLFQYISNKLIGLNTTNFVLPVPMLNVINGGAHADNYIDFQEFMIMPLGAKKMHEALKMASETFHALQNLLKKRGLNTNKGDEGGFAPNLKLAEDALDIMVEAIKLAGYKP", "aa_hexdigest": "fd3aef4d3e8750fb02ce38b7343c7fb2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00125F4A82", "query_cov": 0.9595141700404858, "subject_cov": 0.9753086419753086, "identity": 0.586, "score": 257.0, "evalue": 2.89e-83, "valid": false, "product": "phosphopyruvate hydratase", "ec_ids": [ "4.2.1.11" ], "uniref50_id": "UniRef50_A0A9E2U821", "kegg_orthology_id": "K01689" }, "pscc": { "uniref50_id": "UniRef50_A0A9E2U821", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E2U821" ], "product": "Enolase" }, "genes": [], "id": "KMADJIBOEG_933", "locus": "GCA000292445_00933" }, { "type": "cds", "sequence": "contig_1", "start": 510812, "stop": 511489, "strand": "-", "frame": 3, "gene": "phoU", "product": "phosphate signaling complex protein PhoU", "db_xrefs": [ "COG:COG0704", "COG:P", "GO:0006817", "GO:0030643", "GO:0045936", "RefSeq:WP_014894326.1", "SO:0001217", "UniParc:UPI00027B42BB", "UniRef:UniRef100_UPI00027B42BB", "UniRef:UniRef50_P47649", "UniRef:UniRef90_P47649" ], "nt": "ATGGAAAACATTAATTACCAAATTTTAAAGCGTTCAGAAAAGAAATTATTAAAGCTTTTTTTTGAGTATTTTAAGCACATAATCAATGCACATGAAACATTAAATCAATTACTTTGTGAAGATAATTTAGAAAAACGCAAAGAACTGATTAAAACTATTTATGAAATGGAGGACCAATCTAATAGGTCTGAATTTAAATTAATTAATGAATCAATTTGGACAATCTCTAAAAATTCTCCATTAGCTAGCCATTTACGCCTAACAATCACAATAATTATGTCAAGTCGTGACTTGGAACGCATTTGTGATTATGCTAATAATTTAACTAAGTTTATAGAACATTATTTGCATCTTGATATAAAGATTTTTAATAATCTAGTTACTTTGCATCAAAGTGCGCTCAATAACCTTAAGAAAACCTTTGAAAGTTTACAAGATAAAGAAAAGCCACTTACATTACAATTTGAAAATGCAACCAAAACTATTATTGAATTTGAGAACCAATATCGCCTTGTATTAACAAAATACTATGAAACCATTAATAAAAATGAGGTTACTGAAAGGATTTTCTTAATTGATCTTGTAGCTTCTGTTAAACATATTGAACGGATTAATGATTATTGCTATAACATCATTAAATCCTTTTTATTTATTAAAAATCCTGAAATATTTAATTAG", "aa": "MENINYQILKRSEKKLLKLFFEYFKHIINAHETLNQLLCEDNLEKRKELIKTIYEMEDQSNRSEFKLINESIWTISKNSPLASHLRLTITIIMSSRDLERICDYANNLTKFIEHYLHLDIKIFNNLVTLHQSALNNLKKTFESLQDKEKPLTLQFENATKTIIEFENQYRLVLTKYYETINKNEVTERIFLIDLVASVKHIERINDYCYNIIKSFLFIKNPEIFN", "aa_hexdigest": "ff06c7801f4639e2713c11d6373a2b65", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B42BB", "ncbi_nrp_id": "WP_014894326.1", "uniref100_id": "UniRef100_UPI00027B42BB", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B42BB", "RefSeq:WP_014894326.1", "UniRef:UniRef100_UPI00027B42BB" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B42BB", "uniref90_id": "UniRef90_P47649" }, "psc": { "uniref90_id": "UniRef90_P47649", "gene": "phoU", "product": "phosphate signaling complex protein PhoU", "uniref50_id": "UniRef50_P47649", "cog_id": "COG0704", "cog_category": "P", "go_ids": [ "GO:0006817", "GO:0030643", "GO:0045936" ] }, "pscc": { "uniref50_id": "UniRef50_P47649", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47649" ], "product": "Uncharacterized protein MG409" }, "genes": [ "phoU" ], "id": "KMADJIBOEG_934", "locus": "GCA000292445_00934" }, { "type": "cds", "sequence": "contig_1", "start": 511492, "stop": 511836, "strand": "-", "frame": 1, "gene": "pstB", "product": "Phosphate import ATP-binding protein PstB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2H5ZIP6" ], "nt": "GTGAAAGATAATTTACATAGGAATGCAAACACCCTTTCTGGTGGACAACAACAACGTTTGTGTATTGCGCGTGCTATTGCTTTACAACCAGATGTTCTTTTGATGGATGAACCTACCAGTGCTTTAGACTCAATTGCCACAAACTCTATTGAACTTCTAATTCAACAACTAAAAGAAAAATTCACAATTGTTATTGTTACTCACTCTATGGCTCAAACAATTAGAATAACTGATGAAACGATTTTTTTTGCTGATGGAAGAGTAATTGAACAAGGCACTACAAAACAGATATTTACAAAGCCTAAGCAAAAAGCAACAAATAGTTATATAAGTGGGAAAAATTAG", "aa": "MKDNLHRNANTLSGGQQQRLCIARAIALQPDVLLMDEPTSALDSIATNSIELLIQQLKEKFTIVIVTHSMAQTIRITDETIFFADGRVIEQGTTKQIFTKPKQKATNSYISGKN", "aa_hexdigest": "27bc3ce0688b10abfcd82c5b5794130f", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2H5ZIP6", "query_cov": 0.9912280701754386, "subject_cov": 0.9826086956521739, "identity": 0.628, "score": 135.0, "evalue": 6.34e-39, "valid": false, "product": "Phosphate import ATP-binding protein PstB", "uniref50_id": "UniRef50_A0A2H5ZIP6" }, "pscc": { "uniref50_id": "UniRef50_A0A2H5ZIP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2H5ZIP6" ], "product": "Phosphate import ATP-binding protein PstB" }, "genes": [ "pstB" ], "id": "KMADJIBOEG_935", "locus": "GCA000292445_00935" }, { "type": "cds", "sequence": "contig_1", "start": 511843, "stop": 511998, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGCACTAGTGTTGGCATGGTTTTTCAAAAATTAACTCCTTTTAATTTTTCTATTTTTGAAAACATTGCTTATGGCATAAGAGCACATGGTATTCACAATAAAAATGCTATCAATGAAATAGTAAGACAGGCATTGATATCAGCAGCATTGTGA", "aa": "MRTSVGMVFQKLTPFNFSIFENIAYGIRAHGIHNKNAINEIVRQALISAAL", "aa_hexdigest": "73a271350e22243bf8d8a872801ce725", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5703.6006, "isoelectric_point": 10.266477394104005 }, "id": "KMADJIBOEG_936", "locus": "GCA000292445_00936" }, { "type": "cds", "sequence": "contig_1", "start": 512209, "stop": 512442, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGCTTGAAAAAATTAAGCATTACCGAAAGCTTTATGAACAACAAATCAAAGAATATAAAAAGAAAATTACTGGTTTAAATAATGAAACAGATGCAAATGAAATCTCCCGTATTAAGAATGAAATTGAAATTTCAAACCGTCTAATAAAGATTAAAAACACCAAAGATAATGTCATTAAAAAGGATTTTGATGAAGAAAATGTATTTGAAATTCGAAATTTCAACTTCTGA", "aa": "MKLEKIKHYRKLYEQQIKEYKKKITGLNNETDANEISRIKNEIEISNRLIKIKNTKDNVIKKDFDEENVFEIRNFNF", "aa_hexdigest": "3db2782527b394446fe39654e8b23578", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9433.715899999994, "isoelectric_point": 9.330396461486817 }, "id": "KMADJIBOEG_937", "locus": "GCA000292445_00937" }, { "type": "cds", "sequence": "contig_1", "start": 512466, "stop": 513095, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTAAAAATGTTTTTAACTTTGTAATTGATTCACTAAGTTCAATGCCATCTATTATTTATGGATTATTTGGACTTTCTTTCTTTTTAAGAGTCCTGCAGTTAAGTGCTGGAGGAGCTAATGGTACTAGTTTAATAGCAGGCATTCTAACTATTAGTGTTGTTATATTACCCTTCCTTATAAGAACTTGTCAACAAGCACTAAATAATGTCAGTTGGGATTTAAGAATTAGTGCTTTTGCTTTAGGTATAAGTAAACGTGAAGTTATTTTCAAAATAGTTTTACCTAGTGCTTTGAAAGGATTAATAGTTGCATTAATTTTGTCAATCAACAGAATTATTGCTGAAACTGCACCCTTCTTTATCACTTCAGGGTTATCATCTAGTAATTTATTTCATTTGTCATTGCCAGGTCAAACACTAACAACAAGGATATATGGACAGTTATTTTCTATTAATAGCAATGCAATAAGTGTTATGTTAGAAACATCATTGGTCTCTGTTGTTTTCTTAATTCTTTTAATCTTTTTCAGTTCTTATTTAATCCCGAGTTTATTTTTGTTAAATAAACAAAAATGGCTAGTAATTAAAAGTAAATTTCAGTCCTTTAAATTATGGAAAAGAACATAA", "aa": "MVKNVFNFVIDSLSSMPSIIYGLFGLSFFLRVLQLSAGGANGTSLIAGILTISVVILPFLIRTCQQALNNVSWDLRISAFALGISKREVIFKIVLPSALKGLIVALILSINRIIAETAPFFITSGLSSSNLFHLSLPGQTLTTRIYGQLFSINSNAISVMLETSLVSVVFLILLIFFSSYLIPSLFLLNKQKWLVIKSKFQSFKLWKRT", "aa_hexdigest": "cd4135b6a7b96c29c5415c540ae08a62", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23097.37010000001, "isoelectric_point": 10.48998928070068 }, "id": "KMADJIBOEG_938", "locus": "GCA000292445_00938" }, { "type": "cds", "sequence": "contig_1", "start": 513120, "stop": 513296, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAGCAATTTTGCTGTTTGTATTTATTAATGGAAGTGTTGCTATTAATAATAATGGTTCTACTGTTTTTTCATTTGAAGCTGATTCAACTGGCAGAGCACTAGTAAATACTCTAGTAATTATTTTGATTACTATCACCATTACTTTTCCACTAGCACTTTTAATTGCAATTTGA", "aa": "MLAILLFVFINGSVAINNNGSTVFSFEADSTGRALVNTLVIILITITITFPLALLIAI", "aa_hexdigest": "4b00b38fded486895dad3aca19e75e35", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6151.298899999996, "isoelectric_point": 4.369065666198731 }, "id": "KMADJIBOEG_939", "locus": "GCA000292445_00939" }, { "type": "cds", "sequence": "contig_1", "start": 513570, "stop": 514220, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAAGTTTTATTGTATCAATAGGAGCTTTAATTATTGCTAGTTATATAGGGGTTAGAACTTCATTTTTCCTTGTTTATCGATGCAAACCAAAAATAAGAAAAAAACTTTCACTTATTATTGATATCCTTTCAGGAATACCATCTGTAATTTTTGGATTATTTGCATCACAAATATTAAGCATTTTCTTTCGGGATATCTTGAAATTACCGCCGCTTTCACTTTTAAATGTGATAGCTATGCTTTCTTTTATGATCATTCCTATTGTTATTTCATTAACAACAAATACATTAACTTATGTAAATAACGATCTAATTAGTGTTGTTGTTTCCTTAGGGGAAAATAAAACAAGTGCGATCTACAAAATTATTAAAAAAGAAATTAAACCACAATTAACAGTTATTTTGACCTTAGCCTTTGCGAGAGCAATTAGTGAAACAATGGCTGTTAACTTTGTTTTGCAGAGTGTTAACTATCAAGAGGTAATTAACAACAATCGTTTTTTTACTTCTGATTTAAAAACACTGGGATCAGTTATTTCCACTTTTATTTTTTCAGAAAATGGAGATGAACAGATTAATGGTGTTTTATATATCTTTGGAATCATAATTTTGATATTAGTTTCATTGTTAAATTTCTTTGCCATTTGA", "aa": "MVSFIVSIGALIIASYIGVRTSFFLVYRCKPKIRKKLSLIIDILSGIPSVIFGLFASQILSIFFRDILKLPPLSLLNVIAMLSFMIIPIVISLTTNTLTYVNNDLISVVVSLGENKTSAIYKIIKKEIKPQLTVILTLAFARAISETMAVNFVLQSVNYQEVINNNRFFTSDLKTLGSVISTFIFSENGDEQINGVLYIFGIIILILVSLLNFFAI", "aa_hexdigest": "fc853dcdcdd492bec880a4c58a73b54a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24009.50100000001, "isoelectric_point": 9.518257331848144 }, "id": "KMADJIBOEG_940", "locus": "GCA000292445_00940" }, { "type": "cds", "sequence": "contig_1", "start": 514230, "stop": 514430, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAAAGAAGATTAAAAAACGTTTAAAAAAAGAGAATCTTTTAAGAATCTTTTCAAAGACTTTAGCATTCTTGTTTTTAGTTTTATTTATAAGTTTTTTTGTTTTTCTTTTAACAGAAGCAACAAAAATTGGACCTGATTTTGCAAAGTCCTTGTTTAATCTTGAATTTAATTTAGGTAATAAACAGGCAGGAATTTGA", "aa": "MQKKIKKRLKKENLLRIFSKTLAFLFLVLFISFFVFLLTEATKIGPDFAKSLFNLEFNLGNKQAGI", "aa_hexdigest": "7bb897ee20a5264b8e15e2030168972f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7669.207399999995, "isoelectric_point": 10.358860588073728 }, "id": "KMADJIBOEG_941", "locus": "GCA000292445_00941" }, { "type": "cds", "sequence": "contig_1", "start": 514688, "stop": 515074, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAATGAAAAAGATTACCCTTTAACAAGTTTTCCAAGAACTGGTGGGTCATTTGCTTCAGGAACAGCAGAAGCTTTTTTAAATTTTTCCGCCTTAAAATCAGATAAAACTTTAGATTCTCAAACTAGAGATATTTTGAAAGGAATTATTAATTATGGACCTCTTGCTAAACCTACTAGTGAAACAAATATTGAAGCTTTTAATACTTTTGTAACTAACTTACAAGATCCTAATCTTTTCGGGATGATTTACCTTAGTTTAGGATTTATTCAAAACAACCTTCAAGCTATTAAAAATAATGGTTTTGAGATACTACCAATTAAATATGAGAATAAGGAAGTTTTGCCTTCTAATCAAACTGTTTCCCAAAATGAGTATAAATGA", "aa": "MSNEKDYPLTSFPRTGGSFASGTAEAFLNFSALKSDKTLDSQTRDILKGIINYGPLAKPTSETNIEAFNTFVTNLQDPNLFGMIYLSLGFIQNNLQAIKNNGFEILPIKYENKEVLPSNQTVSQNEYK", "aa_hexdigest": "de5df9895df02f71adb9041ae27c8b70", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14250.831799999985, "isoelectric_point": 5.070288276672362 }, "id": "KMADJIBOEG_942", "locus": "GCA000292445_00942" }, { "type": "cds", "sequence": "contig_1", "start": 515258, "stop": 515563, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTAAAGTTTAGAAATTTCTTTAAATTGACGCTACTAACATTAGCATCTGCTTTTTTTCTTAGTGGTTGTGCTAACATAAACCTGATTAGTGCTGTTGGTTCTTCTTCAGTTCAACCTTTACTAAATAAATTAAGTTCTTATTATGTTCTAAATGAAAATAATGACAATGATAAGCTTGTTGAAATTAGTGTGCAAGCAGGCGGATCAAATGCTGGAATCAGAGCAATCATTAACGGCTTTGCTGATATCGGAAATGTCTCTAAAAACCCTAAGGAATATGCAAAAGAGAATGAAAAAAAATGA", "aa": "MLKFRNFFKLTLLTLASAFFLSGCANINLISAVGSSSVQPLLNKLSSYYVLNENNDNDKLVEISVQAGGSNAGIRAIINGFADIGNVSKNPKEYAKENEKK", "aa_hexdigest": "7a1afa657b5932eb86f633012480eda9", "start_type": "GTG", "rbs_motif": "ATTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10959.39219999999, "isoelectric_point": 9.131189155578614 }, "id": "KMADJIBOEG_943", "locus": "GCA000292445_00943" }, { "type": "cds", "sequence": "contig_1", "start": 515965, "stop": 516708, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTTCATTATCAAAAATAGTTTTAAATCTAAGGATAAGAATATTGATTTAGATATCAACAGTAACTCTTTAACATTTGTGGATAACCCTAAAAAATATTTCAAACATCTAGAGCCTACTAATGAAAAGAAAGGTATATTTTATGTTTTAGCAGAAATAAATAATAGCAATGAACTATTCAAATATGGTTCTGAAAGTGATTCTGAAATTATTAAAGACAAAATGTATTTTCTATCACAAAATCAAAAAAACCCTAAATTAAGAACATATTTATTTAAATTTCATACTAACAAACTTTTTGTTGATAAAAATGACCTGGGATTTAAGTTAGAAAAAGACAAGGTATTTTTATCAGTTGATAACTTAAAAATTGCTGAATTAGATTTAAAGTCATCTTCATTTAAATTTCTAGAGGATTATCAACTTGATTTTCATGAACCTTTTAGTTTAAATGACGAACAATTACTAGTTGATAAACTCAATATTACTCTAAGTGAAAAAAGATTGCAAACAACTAAAAACGTCCGCTTTAACCTAAAGAATAAATTTATTAACATCCATTTAGTAGAAAATAAAAATCAATTTAATTTAGTTTTTGATGTCGATGTTAGATCAAAAAAATTATTTATTAAAGGTGTTAATAATGATAACCAAGTATTTAGTATTAGCTATGATTTAAAAATAACAAATAATCAAACACTTTTAATTGTAGATGCCAATGGTTTTGATAATTCTATTTGA", "aa": "MAFIIKNSFKSKDKNIDLDINSNSLTFVDNPKKYFKHLEPTNEKKGIFYVLAEINNSNELFKYGSESDSEIIKDKMYFLSQNQKNPKLRTYLFKFHTNKLFVDKNDLGFKLEKDKVFLSVDNLKIAELDLKSSSFKFLEDYQLDFHEPFSLNDEQLLVDKLNITLSEKRLQTTKNVRFNLKNKFINIHLVENKNQFNLVFDVDVRSKKLFIKGVNNDNQVFSISYDLKITNNQTLLIVDANGFDNSI", "aa_hexdigest": "d93d3864bc9fca0b3ae220163c7b82e4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28982.705900000044, "isoelectric_point": 8.726843452453611 }, "id": "KMADJIBOEG_944", "locus": "GCA000292445_00944" }, { "type": "cds", "sequence": "contig_1", "start": 516814, "stop": 517452, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATTTGATATTAATTCGCTTGTTAGTCAAACTGAAAAAACTCTAAATAAAGAAACAATAGGTACAAAAATTAAACCAACTATTAAATTTAAAGATAAATTCATAAATGCAATTAAAGAAGTTAGTTTAATTAATCAAAGAATTGATGAATCAATTGATAAAAATGAAGCGTTAAAAAGCTTTAATATTTCAACCAATAATCAAAGTAATTTTTATCCAAATTTAGATTATTTATACAACCTATTGCAAATGTCACCAAACAATAAAGAAGAACTATTCTTTATTAGAAATTTACCAAGAATGGTCAAAACCATTTTTGATAGATCAACATTAACTGTTAAAGTAAAAATTGGTAATTCTGTAAATGAAATAACATTGTTAAGAAATAACAAGAATTATTTTGATTTAAGTTCAATTGAGGAGTTTTTAACAGAAAAACAGAAAGCAGATAATGAAATGAATATTGAATTTTTAGCACTTAATTTTTTTGTAGATGGATATGAATCAGAAAATATTAGTAACTATAGTGTTGAACCACTGTTTGACAGTATTAAAAAACTTTCAACTATCAAAAGAACTAAGGATGGTTTTGAATACAAATTTAAATATAGAAAAGATTTTAATGAACAGCGTTGA", "aa": "MEFDINSLVSQTEKTLNKETIGTKIKPTIKFKDKFINAIKEVSLINQRIDESIDKNEALKSFNISTNNQSNFYPNLDYLYNLLQMSPNNKEELFFIRNLPRMVKTIFDRSTLTVKVKIGNSVNEITLLRNNKNYFDLSSIEEFLTEKQKADNEMNIEFLALNFFVDGYESENISNYSVEPLFDSIKKLSTIKRTKDGFEYKFKYRKDFNEQR", "aa_hexdigest": "9df99f3453272cb1153c648f073701fe", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25017.10970000002, "isoelectric_point": 7.727454185485839 }, "id": "KMADJIBOEG_945", "locus": "GCA000292445_00945" }, { "type": "cds", "sequence": "contig_1", "start": 517462, "stop": 517755, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTATAAATGAGTTTAAAGAGAAAATGAAAAAGGATAGAAAAATCATTGAAGCATTTACTAATAATCGTGAAATAACTCAAACGATATTAAGACTAGGTAATGGATTAGTAGTTAGACCAGAAACTGAAGATATTAGACGTGAAGCATTCAACAGTAAATGGGATATTTTTAAACATACACCTATATTACCTGAACAAGAAGTTGTTCATCATCAAATAAGTTTTTATCTAAGGCAAAATAATCCTGATGAAGTTTTACCAATTAGTTTCAGTAATCTTTCAAAGCTTTGA", "aa": "MSINEFKEKMKKDRKIIEAFTNNREITQTILRLGNGLVVRPETEDIRREAFNSKWDIFKHTPILPEQEVVHHQISFYLRQNNPDEVLPISFSNLSKL", "aa_hexdigest": "cf1a60f4f29f23624657833c1ed74a95", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11522.06609999999, "isoelectric_point": 8.070232963562013 }, "id": "KMADJIBOEG_946", "locus": "GCA000292445_00946" }, { "type": "cds", "sequence": "contig_1", "start": 518542, "stop": 518793, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTTCATATCTTTTTATTCCTTTATTAGTTTCTTTTCTTTTTCCCGTTGCTTTAGCTAATGCTTCACTTGAAAAAAACTTGGTAGATACTAATCTTTATCTCTCTCCAAACACAATTCAAAACAGAAGCTATACAGCTGGATTTAATTTAAAAAACGATTATTTTCGAAAAAGCTTTAATTTTGATAATGAAGTAATTCAAAGACCTAATCAAAATAATTATTTTGCAAAAACTTATTCGAATGAATGA", "aa": "MRSYLFIPLLVSFLFPVALANASLEKNLVDTNLYLSPNTIQNRSYTAGFNLKNDYFRKSFNFDNEVIQRPNQNNYFAKTYSNE", "aa_hexdigest": "e0c9180b16a3e8b9dd28cf214476f950", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9725.829599999994, "isoelectric_point": 9.047960472106933 }, "id": "KMADJIBOEG_947", "locus": "GCA000292445_00947" }, { "type": "cds", "sequence": "contig_1", "start": 518906, "stop": 519400, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002EE4522", "UniRef:UniRef90_UPI0002EE4522" ], "nt": "ATGAATCTTTTAATAGATAAAAAGAAATCTAGCTTAGATCTTTTTCCTAAAGAGATCAAATTAACTACTCTAAAGATAAATAGTTCGCTTCATTTTTATTTAAATGTTGATGCAATTAAAAATCAGCTCAATATTGAAGTTAATATAAGTAAAAATAGATTAACTAGCCTTGTTTATGATATTGCTATCAAAAATGAAAATGAATTGCAAATTAGAACAACAAATAACTACTTAAATAAATATATTTGGTTTGATCTTGATAAGAAAAATAATCAGAAACTGAAAAATGAGCTTAAGTTATTTCTCTCATTAAAAAAATTTCAATTTAAAAAAGAACCTAATTTTAGCTTAAAGAAAAACAGCTATAGTTTTCAAATAGACAAAATTATTCAATCTAATAGTGAAGATAAAAAAACAGATATTATAGTATATTTGATTATTGGTTTTAGTGTTTTAGTTTTGTTTATCACTGTTTTTATTTACTTTCATAAGTGA", "aa": "MNLLIDKKKSSLDLFPKEIKLTTLKINSSLHFYLNVDAIKNQLNIEVNISKNRLTSLVYDIAIKNENELQIRTTNNYLNKYIWFDLDKKNNQKLKNELKLFLSLKKFQFKKEPNFSLKKNSYSFQIDKIIQSNSEDKKTDIIVYLIIGFSVLVLFITVFIYFHK", "aa_hexdigest": "2f74aa835235b30e1b57e012b9b89a87", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0002EE4522", "query_cov": 1.0, "subject_cov": 0.9162011173184358, "identity": 1.0, "score": 303.0, "evalue": 7.19e-104, "valid": true, "uniref50_id": "UniRef50_UPI0002EE4522" }, "pscc": { "uniref50_id": "UniRef50_UPI0002EE4522", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002EE4522" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19476.589199999995, "isoelectric_point": 9.657186698913574 }, "id": "KMADJIBOEG_948", "locus": "GCA000292445_00948" }, { "type": "cds", "sequence": "contig_1", "start": 519578, "stop": 520078, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTTCTATTCAGAAAACTAAAAATGGTTTTCAATATAGTTTAAAGTTTAGATCTGAATACAATGAACATCATATTTTAAAAGATCATTTTTTAATTCCTATTGTTACAAATCAGAAAAATTTCAGTGTTAATGACATTGAAAAAAACGGATTAAATAGTTATCAAAGAGAACAAATTACATATGCAATTAAAAATAGTTTTACTTCTCAAAAAGAAAACAATTTAAATATTTCATCCGCTACAATAAAATACATAAAAGATCCTGAGAAATTAATTAAAAAATCTTTAATTAAGCCCAGTGTTAAAAATGGTATATTCTATGTTTCAGCACAAATAATTAACAGTAATGATTTAACTAAATGGGGTTCTAAAAATGATAGCGAAATCATTAAAGATAAGATGTATTTCTTAGAACAAAATAAAAACTTTCCTGCAATAAGAACTTATCTTTTTCAAATGAGAACCAAAAAGTTAGTTTTAAATGTTAATGATATTTGA", "aa": "MPSIQKTKNGFQYSLKFRSEYNEHHILKDHFLIPIVTNQKNFSVNDIEKNGLNSYQREQITYAIKNSFTSQKENNLNISSATIKYIKDPEKLIKKSLIKPSVKNGIFYVSAQIINSNDLTKWGSKNDSEIIKDKMYFLEQNKNFPAIRTYLFQMRTKKLVLNVNDI", "aa_hexdigest": "1789ac8c6b497b4f70dc97ab62b91294", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19459.105399999997, "isoelectric_point": 9.697866249084473 }, "id": "KMADJIBOEG_949", "locus": "GCA000292445_00949" }, { "type": "cds", "sequence": "contig_1", "start": 520127, "stop": 520771, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002F2F2D0", "UniRef:UniRef90_UPI0002F2F2D0" ], "nt": "TTGATTGATACTAATCTTTATCTAGTTAAAGATGATTTTCAATCACAAAATCAATTAACTATAGCAACAAATCAACTTGCTAAGATCATTGTTAACCAAATTGAATTTGATTCAAATTCTTTAATAGCTAATCCAACTACTGTTTTAAACAAAGAACTAATAGGATCAAAAATAACACCAAAGCTGAAATTTAGTGATCAATTTTCTAATGCAATTGAAATGGTAAGTAAGCTAAATCAAGAGTTTGATCAATTAGCAAATAAAGATAAAACTTTTTTTCAATTTGCTTTAGATTTATTAGAAAAACAGGAAGAATCTAAATTTGATTTTGAACCAAAAGATGAAAGAATTGATGCAATATTTTTCTTAAGTAATTTAATTAACATTAATCCAAAACAAGAGAAAACATTAAATTTTATTCGGATATTACCCAACCTAATTAAAAGTATTTTTAAAGATACTACCATCACGATAAATATAAAGATAGGTGGCAAAAATAAAGTAATTACTTTTATAGAAAATGGCAGTAACGTTTTTTTATTAAGTGATGTTGAAAATTTCTTAAATGCTGATCAAACTGGTATTAATTTTTATGAAATTGAGTTTTTAACTTTTGATTTTATTGTTGTGAATAAAACAGGATGA", "aa": "MIDTNLYLVKDDFQSQNQLTIATNQLAKIIVNQIEFDSNSLIANPTTVLNKELIGSKITPKLKFSDQFSNAIEMVSKLNQEFDQLANKDKTFFQFALDLLEKQEESKFDFEPKDERIDAIFFLSNLININPKQEKTLNFIRILPNLIKSIFKDTTITINIKIGGKNKVITFIENGSNVFLLSDVENFLNADQTGINFYEIEFLTFDFIVVNKTG", "aa_hexdigest": "da89400994f088c781a1f598fc9493ea", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 519824, "stop": 520852, "observations": { "mutation": [ 520129, 520828, 520846 ], "stop": [ 520129, 520828, 520846 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0002F2F2D0", "query_cov": 1.0, "subject_cov": 0.4562899786780384, "identity": 0.995, "score": 407.0, "evalue": 8.9e-140, "gene_start": 1, "gene_end": 214, "reference_start": 28, "reference_end": 241, "reference_sequence": "MSWKRYLKWVSFAIIPLLFANTSIKSKLIDTNLYLVKDDFQSQNQLTIATNQLAKIIVNQIEFDSNSLIANPTTVLNKELIGSKITPKLKFSDQFSNAIEMVSKLNQEFDQLANKDKTFFQFALDLLEKQEESKFDFEPKDERIDAIFFLSNLININPKQEKTLNFIRILPNLIKSIFKDTTITINIKIGGKNKVITFIENGSNVFLLSDVENFLNADQTGINFYEIEFLTFDFIVVNKTGWTLKNQPVDSFFKSVKNLPSIQKTKNGFQYSLKFRSEYNEHHILKDHFLIPIVTNQKNFSVNDIEKNGLNSYQREQITYAIKNSFTSQKENNLNISSATIKYIKDPEKLIKKSLIKPSVKNGIFYVSAQIINSNDLTKWGSKNDSEIIKDKMYFLEQNKNFPAIRTYLFQMRTKKLVLNVNDIWFKSSGDKLRVIVNNVEIDEFNPKENNTSIFWKLWSSYKWLFFFS" }, "paralog": false, "identity": 0.9912280701754386, "score": 656.0, "evalue": 2.89e-240, "description": "Internal stop codon at 520129, 520828, 520846. Nonsense mutation around 520129, 520828, 520846.", "psc": { "uniref90_id": "UniRef90_UPI0002F2F2D0", "uniref50_id": "UniRef50_UPI0002F2F2D0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0002F2F2D0", "UniRef:UniRef50_UPI0002F2F2D0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0002F2F2D0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002F2F2D0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 24550.77500000003, "isoelectric_point": 4.768586921691894 }, "id": "KMADJIBOEG_950", "locus": "GCA000292445_00950" }, { "type": "cds", "sequence": "contig_1", "start": 520991, "stop": 521305, "strand": "-", "frame": 3, "gene": "rpsI", "product": "30S ribosomal protein S9", "db_xrefs": [ "COG:COG0103", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022627", "KEGG:K02996", "SO:0001217", "UniRef:UniRef50_P31782", "UniRef:UniRef90_P47656" ], "nt": "ATGGATAAAAAATCTTTTTATGGACTTGGTCGTCGTAAATCTTCTACTGCTAAAGTTTATTTATATCAAAGCAAAGATAAGGGTAAAATAACCATTAATCATCGTAATCCTAGCGATTATTTTCCAAATAAATTGGTGATTCAAGATATGGAACAACCCTTAGAGTTAACCAAACTTAAGGATAACTTTGATATCAATGTTGTTGTTAAAGGTGGAGGATTTACTGGGCAGGCAGGAGCCATTAGATTAGGTATTGTTCGTGCTTTAATAAAATTTAATCCAGATCTTAAAAAGTTATTAAAAACCAAAAATTAA", "aa": "MDKKSFYGLGRRKSSTAKVYLYQSKDKGKITINHRNPSDYFPNKLVIQDMEQPLELTKLKDNFDINVVVKGGGFTGQAGAIRLGIVRALIKFNPDLKKLLKTKN", "aa_hexdigest": "0b958944b38c6c4823cb1fd53c90a36d", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpsI" ], "truncated": "5-prime", "pseudogene": { "start": 520908, "stop": 521305, "observations": { "deletion": [ 520996 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47656", "query_cov": 0.9903846153846154, "subject_cov": 0.7803030303030303, "identity": 1.0, "score": 206.0, "evalue": 4.69e-67, "gene_start": 1, "gene_end": 103, "reference_start": 1, "reference_end": 103, "reference_sequence": "MDKKSFYGLGRRKSSTAKVYLYQSKDKGKITINHRNPSDYFPNKLVIQDMEQPLELTKLKDNFDINVVVKGGGFTGQAGAIRLGIVRALIKFNPDLKKLLKTKKLTTRDKRAKERKKFGLYGARRAPQFTKR" }, "paralog": false, "identity": 1.0, "score": 259.0, "evalue": 1.63e-90, "description": "Frameshift due to deletion around 520996.", "psc": { "uniref90_id": "UniRef90_P47656", "gene": "rpsI", "product": "30S ribosomal protein S9", "uniref50_id": "UniRef50_P31782", "cog_id": "COG0103", "cog_category": "J", "kegg_orthology_id": "K02996", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022627" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47656", "UniRef:UniRef50_P31782", "COG:COG0103", "COG:J", "KEGG:K02996", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P31782", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P31782" ], "product": "Small ribosomal subunit protein uS9" } }, "id": "KMADJIBOEG_951", "locus": "GCA000292445_00951" }, { "type": "cds", "sequence": "contig_1", "start": 521317, "stop": 521502, "strand": "-", "frame": 1, "gene": "rplM", "product": "50S ribosomal protein L13", "db_xrefs": [ "COG:COG0102", "COG:J", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0017148", "GO:0022625", "KEGG:K02871", "SO:0001217", "UniRef:UniRef50_P47657", "UniRef:UniRef90_P47657" ], "nt": "ATGGGTGGAATTAAAAAAACTAGTGGAAGGGATATGATAAACAAAAATTCAGATAAACTTGTTTTCAATGCTGTTAAGGGAATGTTACCTGATAATCGTTTAAGCAGAAGATTAATAACTAAAGTACATGTTTTTAAGAATGATAAGCACAACATGGAAGCACAAAAACCAACATTACTTAATTGA", "aa": "MGGIKKTSGRDMINKNSDKLVFNAVKGMLPDNRLSRRLITKVHVFKNDKHNMEAQKPTLLN", "aa_hexdigest": "d8458e5909285375f3e86844599ca9a5", "start_type": "ATG", "rbs_motif": null, "genes": [ "rplM" ], "truncated": "3-prime", "pseudogene": { "start": 521317, "stop": 521751, "observations": { "mutation": [ 521523 ], "stop": [ 521523 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47657", "query_cov": 1.0, "subject_cov": 0.4178082191780822, "identity": 1.0, "score": 122.0, "evalue": 1.36e-34, "gene_start": 1, "gene_end": 61, "reference_start": 84, "reference_end": 144, "reference_sequence": "MQKTSMLTKEEAIKNRKWYLVDASGLVLGKLAVKAANLIRGKNKANFTPNQDCGDHLIIINSDQVVLTGNKKDNEFWYHHSQYMGGIKKTSGRDMINKNSDKLVFNAVKGMLPDNRLSRRLITKVHVFKNDKHNMEAQKPTLLNWS" }, "paralog": false, "identity": 0.9930555555555556, "score": 289.0, "evalue": 1.1e-102, "description": "Internal stop codon at 521523. Nonsense mutation around 521523.", "psc": { "uniref90_id": "UniRef90_P47657", "gene": "rplM", "product": "50S ribosomal protein L13", "uniref50_id": "UniRef50_P47657", "cog_id": "COG0102", "cog_category": "J", "kegg_orthology_id": "K02871", "go_ids": [ "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0017148", "GO:0022625" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47657", "UniRef:UniRef50_P47657", "COG:COG0102", "COG:J", "KEGG:K02871", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0017148", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47657", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47657" ], "product": "Large ribosomal subunit protein uL13" } }, "id": "KMADJIBOEG_952", "locus": "GCA000292445_00952" }, { "type": "cds", "sequence": "contig_1", "start": 521521, "stop": 521736, "strand": "-", "frame": 1, "gene": "rplM", "product": "50S ribosomal protein L13", "db_xrefs": [ "COG:COG0102", "COG:J", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0017148", "GO:0022625", "KEGG:K02871", "SO:0001217", "UniRef:UniRef50_P47657", "UniRef:UniRef90_P47657" ], "nt": "ATGCTTACAAAGGAAGAAGCCATTAAAAACAGGAAGTGGTATCTTGTTGACGCTAGTGGTTTGGTTTTAGGCAAATTAGCAGTTAAAGCTGCAAATTTAATTAGAGGAAAAAATAAAGCTAATTTTACTCCTAATCAAGATTGTGGAGATCATCTAATAATTATTAACAGTGATCAAGTGGTTTTAACTGGAAATAAAAAAGACAATGAGTTTTGA", "aa": "MLTKEEAIKNRKWYLVDASGLVLGKLAVKAANLIRGKNKANFTPNQDCGDHLIIINSDQVVLTGNKKDNEF", "aa_hexdigest": "c96929e361c5c3fd078cf3c5ea4e51fe", "start_type": "ATG", "rbs_motif": "ATATA", "genes": [ "rplM" ], "truncated": "5-prime", "pseudogene": { "start": 521317, "stop": 521751, "observations": { "mutation": [ 521523 ], "stop": [ 521523 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47657", "query_cov": 1.0, "subject_cov": 0.4863013698630137, "identity": 1.0, "score": 145.0, "evalue": 2.2e-43, "gene_start": 1, "gene_end": 71, "reference_start": 6, "reference_end": 76, "reference_sequence": "MQKTSMLTKEEAIKNRKWYLVDASGLVLGKLAVKAANLIRGKNKANFTPNQDCGDHLIIINSDQVVLTGNKKDNEFWYHHSQYMGGIKKTSGRDMINKNSDKLVFNAVKGMLPDNRLSRRLITKVHVFKNDKHNMEAQKPTLLNWS" }, "paralog": false, "identity": 0.9930555555555556, "score": 289.0, "evalue": 1.59e-102, "description": "Internal stop codon at 521523. Nonsense mutation around 521523.", "psc": { "uniref90_id": "UniRef90_P47657", "gene": "rplM", "product": "50S ribosomal protein L13", "uniref50_id": "UniRef50_P47657", "cog_id": "COG0102", "cog_category": "J", "kegg_orthology_id": "K02871", "go_ids": [ "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0017148", "GO:0022625" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47657", "UniRef:UniRef50_P47657", "COG:COG0102", "COG:J", "KEGG:K02871", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0017148", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47657", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47657" ], "product": "Large ribosomal subunit protein uL13" } }, "id": "KMADJIBOEG_953", "locus": "GCA000292445_00953" }, { "type": "cds", "sequence": "contig_1", "start": 521789, "stop": 521905, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGAACTTAACCCAATTGGAAAACTTAGCTAAGCCTTTTTTAAAAGAAAAGAAACGCTCTTTATCACAAAAAATGGTGGATAAATACTTTAAAGGACTTTTTGAAGAAAAGTAA", "aa": "MLNLTQLENLAKPFLKEKKRSLSQKMVDKYFKGLFEEK", "aa_hexdigest": "c693fa80aa9868ed8366ba16f4a65cf7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4574.410099999999, "isoelectric_point": 9.698317527770996 }, "id": "KMADJIBOEG_954", "locus": "GCA000292445_00954" }, { "type": "cds", "sequence": "contig_1", "start": 522248, "stop": 523354, "strand": "-", "frame": 3, "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "db_xrefs": [ "COG:COG2812", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0006261", "GO:0009360", "GO:0016887", "GO:0046872", "KEGG:K02343", "SO:0001217", "UniRef:UniRef50_P47658", "UniRef:UniRef90_P47658" ], "nt": "GTGTGTAAAAGTATTAACACTAATAGTGCCATTGATATAGTTGAGATAGATGCAGCTTCTAAAAATGGTATTAATGATATTAGAGAGTTGGTAGAAAATGTTTTCAATCATCCCTTCACATTTAAAAAAAAGGTTTATATTTTAGATGAAGCACACATGTTAACCACCCAATCATGGGGTGGCTTGTTAAAAACTTTAGAAGAATCACCACCTTATGTTCTTTTTATTTTTACAACTACTGAATTTAACAAGATTCCATTAACAATTTTGTCCAGATGTCAAAGCTTCTTTTTTAAAAAAATAACTAGTGATTTAATCCTTGAAAGATTAAATGATATAGCAAAAAAAGAAAAGATTAAGATAGAGAAAGATGCATTAATAAAAATTGCTGATTTATCCCAAGGTTCATTGCGTGATGGGCTTAGCTTACTAGATCAAATTAGCAATTTCTCTGATAGTGAAAAAATTAGTATTACTGATGTTGAAAAAACATTTAATATCGTTGATAGAAATGCAAAATTTACTTTTATTAAAGCAGTTTTATCAGGAGATATAAAAGAAGCATTTAACTTGCTTGATGATTTTGAAAGTAATGGTCTAAATTTCACTTACTTCTTAAGAGAGTTGTTTGCGCTAACAGTTAATTTATATGCGTATGCAAAACTAAAAAATATTAATGTTTTAGATTCAACTGAAAAAACAATGATAGAAACACTTAATTTTGAAAAGCAGCATTATGCGTTTTTAATTAAGGCCATTGAAGAAAATACTAATTTTGGTTTAAGTCAATTAACTTTAATTGATAGGCTAAAAGCTATTGTTATTAGCTATAATGAATTTTTTAATCAAAAACCTTTAACAATAAGCTCACCAAGTAATGAAAAATCTCTACACTTAGAAACTGAATATTTGGAAAAGAAAAAAATAAAAAAATCAAATCACAAACAAGACCAAAAGCACTTTTCTTTATTTGAAAAATCTTTTATAGATAAATCAGAAAAGACACCTAAAAATGATGAAGTTACTAATAACAAATTCTTAGATACAAGCAAACTAAATCTTGCCAATATTGCTCTTGCAATTAATGCATTTAATGACAATAAATGA", "aa": "MCKSINTNSAIDIVEIDAASKNGINDIRELVENVFNHPFTFKKKVYILDEAHMLTTQSWGGLLKTLEESPPYVLFIFTTTEFNKIPLTILSRCQSFFFKKITSDLILERLNDIAKKEKIKIEKDALIKIADLSQGSLRDGLSLLDQISNFSDSEKISITDVEKTFNIVDRNAKFTFIKAVLSGDIKEAFNLLDDFESNGLNFTYFLRELFALTVNLYAYAKLKNINVLDSTEKTMIETLNFEKQHYAFLIKAIEENTNFGLSQLTLIDRLKAIVISYNEFFNQKPLTISSPSNEKSLHLETEYLEKKKIKKSNHKQDQKHFSLFEKSFIDKSEKTPKNDEVTNNKFLDTSKLNLANIALAINAFNDNK", "aa_hexdigest": "ea6d560d97375f74a2b53a47aee34959", "start_type": "GTG", "rbs_motif": null, "genes": [ "dnaX" ], "truncated": "both", "pseudogene": { "start": 521945, "stop": 523582, "observations": { "mutation": [ 522049, 522250, 523387 ], "stop": [ 522049, 522250, 523387 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47658", "query_cov": 1.0, "subject_cov": 0.6164154103852596, "identity": 0.997, "score": 697.0, "evalue": 1.4e-249, "gene_start": 1, "gene_end": 368, "reference_start": 77, "reference_end": 444, "reference_sequence": "MHQVFYQKYRPINFKQTLGQESIRKILVNAINRDKLPNGYIFSGERGTGKTTFAKIIAKAINCLNWDQIDVCNSCDVCKSINTNSAIDIVEIDAASKNGINDIRELVENVFNHPFTFKKKVYILDEAHMLTTQSWGGLLKTLEESPPYVLFIFTTTEFNKIPLTILSRCQSFFFKKITSDLILERLNDIAKKEKIKIEKDALIKIADLSQGSLRDGLSLLDQISNFSDSEKISITDVEKTFNIVDRNAKFTFIKAVLSGDIKEAFNLLDDFESNGLNFTYFLRELFALTVNLYAYAKLKNINVLDSTEKTMIETLNFEKQHYAFLIKAIEENTNFGLSQLTLIDRLKAIVISYNEFFNQKPLTISSPSNEKSLHLETEYLEKKKIKKSNHKQDQKHFSLFEKSFIDKSEKTPKNDEVTNNKFLDTSKLNLANIALAINAFNDNKWINHFQNLLSVFQTKFNDKDKQNNLSYFNNFIDKYSARDIVKATKIVKASSFGIVILFEDQKIAMRLWKEAIEEGNVQATIFQIFNQNLFLASFSEHQYKTTITEETKNQKYQTEVLNLTQLENLAKPFLKEKKRSLSQKMVDKYFKGLFEEK" }, "paralog": false, "identity": 0.9908256880733946, "score": 1054.0, "evalue": 0.0, "description": "Internal stop codon at 522049, 522250, 523387. Nonsense mutation around 522049, 522250, 523387.", "psc": { "uniref90_id": "UniRef90_P47658", "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_P47658", "cog_id": "COG2812", "cog_category": "L", "kegg_orthology_id": "K02343", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0006261", "GO:0009360", "GO:0016887", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47658", "EC:2.7.7.7", "UniRef:UniRef50_P47658", "COG:COG2812", "COG:L", "KEGG:K02343", "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0006261", "GO:0009360", "GO:0016887", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47658", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47658" ], "product": "DNA polymerase III subunit gamma/tau" } }, "id": "KMADJIBOEG_955", "locus": "GCA000292445_00955" }, { "type": "cds", "sequence": "contig_1", "start": 523385, "stop": 523582, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00004.35", "PFAM:PF13177.12" ], "nt": "ATGCACCAAGTTTTTTATCAAAAATATCGGCCAATCAATTTCAAACAAACCCTAGGACAAGAATCGATAAGAAAAATCTTGGTGAATGCTATTAACAGGGATAAACTACCTAATGGTTATATCTTTTCAGGTGAAAGAGGAACAGGTAAAACTACTTTTGCAAAGATAATAGCAAAAGCGATAAACTGCTTAAATTGA", "aa": "MHQVFYQKYRPINFKQTLGQESIRKILVNAINRDKLPNGYIFSGERGTGKTTFAKIIAKAINCLN", "aa_hexdigest": "d3470f6a56b9b116dda894a5ae7cc9fc", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028A06DEE", "query_cov": 0.9538461538461539, "subject_cov": 0.96875, "identity": 0.5, "score": 69.3, "evalue": 2.39e-14, "valid": false, "product": "AAA family ATPase", "uniref50_id": "UniRef50_A0A7V9ZZE4" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00004.35", "name": "AAA", "length": 22, "aa_cov": 0.3384615384615385, "hmm_cov": 1.0, "evalue": 7.610217365548407e-06, "score": 21.570632934570312, "start": 41, "stop": 62 }, { "id": "PF13177.12", "name": "DNA_pol3_delta2", "length": 46, "aa_cov": 0.7076923076923077, "hmm_cov": 1.0, "evalue": 1.8365153760282595e-09, "score": 32.901039123535156, "start": 19, "stop": 64 } ], "seq_stats": { "molecular_weight": 7458.626899999999, "isoelectric_point": 10.176801872253417 }, "id": "KMADJIBOEG_956", "locus": "GCA000292445_00956" }, { "type": "cds", "sequence": "contig_1", "start": 523588, "stop": 525222, "strand": "-", "frame": 1, "gene": null, "product": "UvrABC system protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PGE6" ], "nt": "ATGTCTGAAATAACATGTAAAAAGTGTCATGGAAAAAAATTAATAAAAGACGCTTTAAGTGTTAAGTTAGGAGGAATTGACATTATTAGCTTTACTGAACTTTCCATTGATAAAAGTATTGATTTTCTATTAAAACTAGAGTTAAATGATGAGCAAAAGAAGATCGGTGAATTAGCTTTAAAAGAGATTATTAATCGTCTTTCTTTTCTTAAAAATGTTGGTTTAGATTATCTTAATCTTGCAAGAAGAGCTTCTACGCTGTCAGGTGGAGAAGCACAAAGAATTAGATTAGCTACCCAAATTGGTTCTCAACTTACTGGTGTTTTATATGTAATGGATGAACCTTCTATTGGATTGCATCAAAAAGACAATATGCGTTTAATTAAAACAATGATGGTAATGCGTGATTTAGGTAACACCTTATTAGTAGTTGAGCATGATAGTGAAACAATGTTAGCGGCAGATTATTTAATTGATATTGGTCCTAAAGCAGGTAATGAAGGTGGTGAATTAGTTGCTTGCGGTACACCTTTACAAGTAATGGAAAACTCAAACTCAATTACTGGACAATATCTTAGTGGTAAAAAACAAATCTTCATTCCAAAAAATAGACATAGTGGTAATGGTAAAACAATTATTATCAAGGGTGCTAAAGTTAATAATTTGAAAAATATTAATGTTACGATTCCTTTAAATAAATTGGTTTTGATAACAGGAGTTTCAGGTTCTGGAAAATCCTCTTTAATTAATCAAACATTAGTTCCAGCTTTAGAAAGAATTCTTTATCGTAAAGGTGTTAAAAAAGATACATATAAGGAAATAATTGGTGCTAACAACATTGATAAGATAATTGTTGTCTCTCAAGACCCAATTGGTAGAACACCACGTTCTAATCCTGCAACCTATATTAGTGTTTTTGATGATATTCGTGATTTATTTGCCAACACAAAAGAAGCTAAAGCAAGAGGATATACAAATTCACGATTTTCTTTTAATGTTCCAGGTGGTAGGTGTGATAAGTGTTTTGGTGATGGTGTGATTCGCATTGAAATGCATTTTTTACCTGATGTTTATGTCAAATGTGAAGTATGTAATGGCAAGAAGTACAATTCACAAACACTGGAAATTAAATATTTGGGAAAATCAATTTTTGATGTTTTACAAATGTCTTGTAAAGAAGCTTATGAATTTTTTAAAGCTATCCCAAATATATCACGTAAACTAAGGTTGTTATGTGATGTTGGTTTAGAATATTTGCAATTAGGTATTAATGTCACTTTTCTTTCAGGTGGGGAAGCACAGAGAATTAAGTTATCTAAGTTTTTACAAAAAAAATCTACTGGTAAAACTTTGTTTGTTTTAGATGAACCCTCTACTGGCTTACATTTAGAAGATATAAACAAACTATTAACAATAATTCAAAGAATCATTAAGAATGGTGATACAGTAGTTGTTATAGAACATAACTTAGATATTATTAAGGTTGCTGACTATATCATTGATTTAGGTCCTGAAGGTGGTGACAATGGTGGTCAAATTGTTGCTCAAGGAACACCTGAACAACTTATAAACCAAGTTAATAAATCTTATACTGCCCAATATTTGTCCAAAATTTTAAAACCAGATTCAATTTAA", "aa": "MSEITCKKCHGKKLIKDALSVKLGGIDIISFTELSIDKSIDFLLKLELNDEQKKIGELALKEIINRLSFLKNVGLDYLNLARRASTLSGGEAQRIRLATQIGSQLTGVLYVMDEPSIGLHQKDNMRLIKTMMVMRDLGNTLLVVEHDSETMLAADYLIDIGPKAGNEGGELVACGTPLQVMENSNSITGQYLSGKKQIFIPKNRHSGNGKTIIIKGAKVNNLKNINVTIPLNKLVLITGVSGSGKSSLINQTLVPALERILYRKGVKKDTYKEIIGANNIDKIIVVSQDPIGRTPRSNPATYISVFDDIRDLFANTKEAKARGYTNSRFSFNVPGGRCDKCFGDGVIRIEMHFLPDVYVKCEVCNGKKYNSQTLEIKYLGKSIFDVLQMSCKEAYEFFKAIPNISRKLRLLCDVGLEYLQLGINVTFLSGGEAQRIKLSKFLQKKSTGKTLFVLDEPSTGLHLEDINKLLTIIQRIIKNGDTVVVIEHNLDIIKVADYIIDLGPEGGDNGGQIVAQGTPEQLINQVNKSYTAQYLSKILKPDSI", "aa_hexdigest": "a620544219b53febaac8ed3343e31d26", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PGE6", "query_cov": 0.9301470588235294, "subject_cov": 0.9980276134122288, "identity": 0.6859999999999999, "score": 709.0, "evalue": 2.08e-252, "valid": false, "gene": "uvrA", "product": "excinuclease ABC subunit UvrA", "uniref50_id": "UniRef50_A0A3B0PGE6", "kegg_orthology_id": "K03701", "go_ids": [ "GO:0003677", "GO:0006289", "GO:0009380", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PGE6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PGE6" ], "product": "UvrABC system protein A" }, "genes": [], "id": "KMADJIBOEG_957", "locus": "GCA000292445_00957" }, { "type": "cds", "sequence": "contig_1", "start": 525466, "stop": 525984, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17760.6" ], "nt": "GTGCAATTATTAGCACCTACTGTTAAAAATCAGCGCGGCATTTTTACAAATGAATTTATTAAATACAAGCAATTAGGTTTTCTTAGAGTCTTAGTTGATGGCCAGATTTACACCTTAGATGATGAAATTAAACTTGATAAAAATACTAAACACAACATTAGTGTAGTGATCGATAGAATTATCATCAATAAAGATAATCAAACTTATTCAAGGATAGTTGATAGCATTGAAACCATTGATAGGTTAACTAATGGCAAGATAGAAGTTCTTAAGGAAGATGGAACAATATTAAATTTCAGCAAAAATCATGGTTGTGATAAATGTGGTTTTTCTATTAGTGAATTGGAACCAAGATTATTTTCCTTTAACTCCCCTTTAGGTTCATGTTCATATTGCAAAGGACTTGGTTTTAGTTATGAACCTGATGTAGACAAGATAATTGCTGATTCTAAACTTTCTATTAACCAAGGAGCCATTGATATTTTTAAAAATATTGTGCATGGAACTTCTTTGGATTGA", "aa": "MQLLAPTVKNQRGIFTNEFIKYKQLGFLRVLVDGQIYTLDDEIKLDKNTKHNISVVIDRIIINKDNQTYSRIVDSIETIDRLTNGKIEVLKEDGTILNFSKNHGCDKCGFSISELEPRLFSFNSPLGSCSYCKGLGFSYEPDVDKIIADSKLSINQGAIDIFKNIVHGTSLD", "aa_hexdigest": "b8f3495ab2700b05fcf00e9b121af830", "start_type": "GTG", "rbs_motif": "ATTTA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17760.6", "name": "UvrA_inter", "length": 92, "aa_cov": 0.5348837209302325, "hmm_cov": 0.9782608695652174, "evalue": 6.917158315683834e-29, "score": 95.11275482177734, "start": 1, "stop": 92 } ], "seq_stats": { "molecular_weight": 19364.896699999994, "isoelectric_point": 5.9281023025512685 }, "id": "KMADJIBOEG_958", "locus": "GCA000292445_00958" }, { "type": "cds", "sequence": "contig_1", "start": 526424, "stop": 526687, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTATTTAGTGATTTAAACAAAATTAACAAAAATGAAAGTCTAAAGCTTTACTTGCTATTTAAACTTTTGTTAAATCCTAAGTTACTAATGATAAACTGCTGCAATGATTTCACTAACCACGCTTATAATTTCATTCGTGGACTTTTGATCTCATATCAAAATAAACAAGGTATTGCGCTTATTTTCAATGATCCTAATAATAAGTTAGTTAAAAATTTTTCAATTAAAAACATTGACTTTGAAACCGGAATGAAAAAATAA", "aa": "MLFSDLNKINKNESLKLYLLFKLLLNPKLLMINCCNDFTNHAYNFIRGLLISYQNKQGIALIFNDPNNKLVKNFSIKNIDFETGMKK", "aa_hexdigest": "429b3469fa69df6b62b9c4719dd5368c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10172.93089999999, "isoelectric_point": 9.517290306091308 }, "id": "KMADJIBOEG_959", "locus": "GCA000292445_00959" }, { "type": "cds", "sequence": "contig_1", "start": 526688, "stop": 526957, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGATATTGTTTTTTAAACAAAACATTACACTTAAGACTTAAAAAAACAAACAAGAAAATTAAGAGAACTTTTTTTATTGATTCAGAAGAGTGCAATATCAATCGTTTAGAAGCTTCAAATAAGCTTTATCTTAATGTTTTGAATTCACAATTAAAAACAATTAATTTCTTTTTAAGATCACAAAAAAATCTTAAAAAACTTAACTTTATTAACAACATTAATTTATTGATAAATCAAACCAAAAAGAATGGTATTAGCAGCTGA", "aa": "MKRYCFLNKTLHLRLKKTNKKIKRTFFIDSEECNINRLEASNKLYLNVLNSQLKTINFFLRSQKNLKKLNFINNINLLINQTKKNGISS", "aa_hexdigest": "c6435208cd0eac410aca869cb3e02f24", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10620.432999999986, "isoelectric_point": 10.466264915466308 }, "id": "KMADJIBOEG_960", "locus": "GCA000292445_00960" }, { "type": "cds", "sequence": "contig_1", "start": 527408, "stop": 527761, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTGCACGTTTCATTCTAACGAAAAAAGAGGTTCATGAATTTTTGATCAAAGCACGTTTATCTTTTATTAAAAATTTTGTTAAACAATCAAACAATTTATATGTTCATGAAAAATTAGTTAATGAACTAAAAGAAAACATCTTATTAAATCAAAATCATTATAGAGGTAAGCATAATAACAGTTTTTCAGTTCTAATCCAAAAAACAGTTTTCAAACAGTTTATTGATACAGTGCAATATGCAATTGGTTTAATCAGCATGAGAAAGAGTTTGGAGACTTTTAACTTTGCATTTCAAATAAAGCAATATTTTTATGAAAGCTTATTGGCTAATTACCAAAAAATTCACTGA", "aa": "MSARFILTKKEVHEFLIKARLSFIKNFVKQSNNLYVHEKLVNELKENILLNQNHYRGKHNNSFSVLIQKTVFKQFIDTVQYAIGLISMRKSLETFNFAFQIKQYFYESLLANYQKIH", "aa_hexdigest": "84e364a5f454017233827f7d926ac2da", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14021.152999999988, "isoelectric_point": 9.859166145324707 }, "id": "KMADJIBOEG_961", "locus": "GCA000292445_00961" }, { "type": "cds", "sequence": "contig_1", "start": 527795, "stop": 527953, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTTTGAAAGAAATTAAGAAGATGGATTTACTTTCTCCAAAATTACTTGTAGAAACTATTGATGAAAGTATTAATTTAGTCAATAAAATCTTTGCTAATATTCAAAATCTTTATCAAGAAATTATCAGCTTAAAATCAGGTCCTAATTTGAACTGA", "aa": "MLLKEIKKMDLLSPKLLVETIDESINLVNKIFANIQNLYQEIISLKSGPNLN", "aa_hexdigest": "cf70471baf11fb5880d27ccd093e9a8b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5941.006799999998, "isoelectric_point": 6.090263938903808 }, "id": "KMADJIBOEG_962", "locus": "GCA000292445_00962" }, { "type": "cds", "sequence": "contig_1", "start": 528041, "stop": 528478, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAATTGAAAATGTTAATCAAAAAACTAGTAAAGTCATTAATCAGTTTTTTACATCAAAATTTATTAATTCAATCTACTTTGAACAAGTTGATTTTGAATACAATAATTTTTTAACTTTAATTGAAAAAAACGAAAGAGAACTTGTTGATTTTAATTGCATGTTTTTCAATCAATTATTTACTTTTAATAACAAAGTAATAAAAAGTTTTTTTGAGCGTTACTATGCATTGAACGAACAAAAAAATAACTTAAAAAACAAACTGAAAATAGCTGCTTATGAAGCTAAATCACGAGATAACAGTTATTTTGAAAAATTGCAAATTGAAAGAGCTAAACAAAGACTTTTTGATGCTAAGAAAACATTCTCAAAACAACATAAAAATGCTTTGCAATTAATTAATTCATTTACCTTTTCTTTAATTTTTAGTGGTTGA", "aa": "MKIENVNQKTSKVINQFFTSKFINSIYFEQVDFEYNNFLTLIEKNERELVDFNCMFFNQLFTFNNKVIKSFFERYYALNEQKNNLKNKLKIAAYEAKSRDNSYFEKLQIERAKQRLFDAKKTFSKQHKNALQLINSFTFSLIFSG", "aa_hexdigest": "c9c279d5abd121adf29633adf5310fc5", "start_type": "TTG", "rbs_motif": "ATTTA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17488.78919999999, "isoelectric_point": 9.542755317687988 }, "id": "KMADJIBOEG_963", "locus": "GCA000292445_00963" }, { "type": "cds", "sequence": "contig_1", "start": 528524, "stop": 528715, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGTTAATAACCATCAATATTTACCGCTTCAAAAAATTAAGTTTAATTTATGTGAAGAGATAGATTTAAGAAATAAAAATAAGTGGTTATTTCCTAATTCATTTAGCATTTTTCTAAAACAAGATTTAAGTTATAAAAAAGATCAAAATTACTTTAATTTAGTGAATATACCTAAACAGTTAAAGTGA", "aa": "MLVNNHQYLPLQKIKFNLCEEIDLRNKNKWLFPNSFSIFLKQDLSYKKDQNYFNLVNIPKQLK", "aa_hexdigest": "47b12d9341e17987ac73c366ebaa765d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7716.933899999998, "isoelectric_point": 9.56325626373291 }, "id": "KMADJIBOEG_964", "locus": "GCA000292445_00964" }, { "type": "cds", "sequence": "contig_1", "start": 528767, "stop": 528931, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAAGAAAATATCTCTTTTTGATAATCTAGAACAGTGGGTTGATTTGTCATTATTAATGATTCGTACCTCCTTTTTAGATGCTAACTATTTTGGGGTTGCAAAGCTATCATTATTTAAGTATGGTAACTATCTCATTGATAATTTAAATCAAGCACAATGA", "aa": "MLKKISLFDNLEQWVDLSLLMIRTSFLDANYFGVAKLSLFKYGNYLIDNLNQAQ", "aa_hexdigest": "bb841f550bd964534f6c153e74b74ce0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6317.288900000001, "isoelectric_point": 5.955214500427247 }, "id": "KMADJIBOEG_965", "locus": "GCA000292445_00965" }, { "type": "cds", "sequence": "contig_1", "start": 528969, "stop": 530414, "strand": "-", "frame": 2, "gene": "rnjA", "product": "putative protein MG423", "db_xrefs": [ "COG:COG0595", "COG:J", "GO:0003723", "GO:0005886", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47662", "UniRef:UniRef90_P47662" ], "nt": "TTGTTCCATACAGTAGGATTTTTTCCTATTTACACAAGTTCAATAGGCGCTTCTATAATTAAAAGCAAAATTAATGAAAACAAACTTAATATTGCACGTGATAAATTGGAAATTCATGAATTAAAACCACTTGAAACAATCGAAATTTCTAATCATTCTATTACCCCTTTTAAAGTATCAAGTTCTTTACCATCATCATTTGGATTTGCTTTAAATACTGATAATGGTTACATCGTTTTTATTGATGATTTCATTGTTTTAAATGATAAGAACATTGCTTTTGAAAATCAACTAAATCAAATTATCCCTAAACTTTCTGACAATACATTGTTATTAATAACAGGAGTTGGTTTAGTTGGTAGAAACAGTGGTTTTACAACACCTAAACACAAGTCATTAGAACAGTTAAACAGGATCATAACTCCAGCCAAAGGCAGAATATTTGTAGCTTGTTATGATTCAAATGCTTACAGTGTTATGACTCTTGCCCAGATTGCAAGGATGCAAAATCGTCCTTTTATTATCTATTCACAATCATTTGTGCATCTCTTTAACACAATTGTGCGTCAAAAATTGTTTAATAACACGCATTTAAATACCATTTCTATTGAGGAGATTAACAATTCAACCAACAGTATTGTTGTTCTCACCTCACCACCTGATAAGTTGTATGCTAAATTATTCAAAATTGGAATGAACGAAGATGAAAGAATTCGTTATCGTAAGAGTGATACTTTTATCTTTATGACTCCTAAAGTTGCTGGCTATGAAGAGATAGAAGCACAAATCCTTGATGATATTGCACGTAATGAAGTTAGTTATTACAACTTAGGTAGAGAAATCCTTTCTATCCAAGCAAGTGATGAAGATATGAAGTTTTTAGTTAGTTCTTTAAAACCAAAATATATTATTCCAACTGGTGGACTTTATAGGGATTTTATAAACTTCACAATGGTTTTAAAACAAGCTGGCGCTGAGCAAAATCAAATCTTAATTCCTTTTAACGGTGAAGTTTTAACTATTGAAAACAAAAAACTAGATAGCAAAAAAAATGAATTGAAATTAAATCCTAAGTGTGTTGATTCTGCTGGATTACAAGAAATTGGAGCTAGCATTATGTTTGAACGTGATCAAATGTCAGAATCTGGAGTTGTGATCATAATCATTTATTTTGATCAAAAAAAGAGTGAATTCTTAAATGAGATAACTTATTCTTTTTTAGGAGTATCTTTAGATGTACCAGAAAAAGATAAACTAAAAACAAAAATGGAAGAATTAATTAAAAAACAAATTAATGATATTAAGGATTTCACCACGATTAAAAAACGAATAGGTAAAGAAATTTCAAAAGAATTGAAGGTTTCTATTAAACGAGCAGTAATGAATTTATTCACCAAGATGACATCAAAAGCACCCTTAATTCTTTCAACTATTATTTCTATTTAA", "aa": "MFHTVGFFPIYTSSIGASIIKSKINENKLNIARDKLEIHELKPLETIEISNHSITPFKVSSSLPSSFGFALNTDNGYIVFIDDFIVLNDKNIAFENQLNQIIPKLSDNTLLLITGVGLVGRNSGFTTPKHKSLEQLNRIITPAKGRIFVACYDSNAYSVMTLAQIARMQNRPFIIYSQSFVHLFNTIVRQKLFNNTHLNTISIEEINNSTNSIVVLTSPPDKLYAKLFKIGMNEDERIRYRKSDTFIFMTPKVAGYEEIEAQILDDIARNEVSYYNLGREILSIQASDEDMKFLVSSLKPKYIIPTGGLYRDFINFTMVLKQAGAEQNQILIPFNGEVLTIENKKLDSKKNELKLNPKCVDSAGLQEIGASIMFERDQMSESGVVIIIIYFDQKKSEFLNEITYSFLGVSLDVPEKDKLKTKMEELIKKQINDIKDFTTIKKRIGKEISKELKVSIKRAVMNLFTKMTSKAPLILSTIISI", "aa_hexdigest": "82c0da418d06d27d1112b8e0b90ba65e", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47662", "query_cov": 1.0, "subject_cov": 0.857397504456328, "identity": 0.996, "score": 915.0, "evalue": 0.0, "valid": true, "gene": "RnjA", "product": "Uncharacterized protein MG423", "uniref50_id": "UniRef50_P47662", "cog_id": "COG0595", "cog_category": "J", "go_ids": [ "GO:0005886", "GO:0046872", "GO:0003723" ] }, "pscc": { "uniref50_id": "UniRef50_P47662", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47662" ], "product": "Uncharacterized protein MG423" }, "genes": [ "rnjA" ], "id": "KMADJIBOEG_966", "locus": "GCA000292445_00966" }, { "type": "cds", "sequence": "contig_1", "start": 530493, "stop": 530654, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002F5EBD4", "UniRef:UniRef90_UPI0002F5EBD4" ], "nt": "ATGGCTAAGATTAAATTCTTTGCACTCGGCGGTCAAGATGAACGTGGTAAAAATTGCTATGTGCTTGAAATTGACAATGATGTGTTTATCTTTAATGTTGGTAGCTTAACACCAACAACAGCAGTTTTAGGTGTTAAAAAAATTATTCCTGATTTTAGTTGA", "aa": "MAKIKFFALGGQDERGKNCYVLEIDNDVFIFNVGSLTPTTAVLGVKKIIPDFS", "aa_hexdigest": "c9b5373594c7ede41c36b5c1622af55e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0002F5EBD4", "query_cov": 1.0, "subject_cov": 0.803030303030303, "identity": 1.0, "score": 110.0, "evalue": 7.18e-31, "valid": true, "uniref50_id": "UniRef50_UPI0002F5EBD4" }, "pscc": { "uniref50_id": "UniRef50_UPI0002F5EBD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002F5EBD4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5821.718500000001, "isoelectric_point": 6.024103355407715 }, "id": "KMADJIBOEG_967", "locus": "GCA000292445_00967" }, { "type": "cds", "sequence": "contig_1", "start": 530657, "stop": 530917, "strand": "-", "frame": 3, "gene": "rpsO", "product": "30S ribosomal protein S15", "db_xrefs": [ "COG:COG0184", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "RefSeq:WP_009885608.1", "SO:0001217", "UniParc:UPI0000134D0F", "UniRef:UniRef100_P47663", "UniRef:UniRef50_P47663", "UniRef:UniRef90_P47663" ], "nt": "ATGAAAATTGATAAAGAACAAATCATTAAGGCTCATCAACTTCACAAAAACGATGTTGGCAGTGTGCAAGTACAAATCTCTATATTAACAGATCAGATTAAAAAATTAACAGACCACCTGTTAGCAAACAAAAAGGATTTTATTTCTAAGCGTGGTTTATATACAAAGGTATCAAAAAGAAAACGGCTACTTAAATATCTAAAAGAACGTAATATTGAAACCTACCGTGATTTGATTAAAAATTTAAACCTCCGTGGTTAA", "aa": "MKIDKEQIIKAHQLHKNDVGSVQVQISILTDQIKKLTDHLLANKKDFISKRGLYTKVSKRKRLLKYLKERNIETYRDLIKNLNLRG", "aa_hexdigest": "6aeee8d790f9c955a6872e2df8a5c4fe", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134D0F", "ncbi_nrp_id": "WP_009885608.1", "uniref100_id": "UniRef100_P47663", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134D0F", "RefSeq:WP_009885608.1", "UniRef:UniRef100_P47663" ] }, "ips": { "uniref100_id": "UniRef100_P47663", "uniref90_id": "UniRef90_P47663" }, "psc": { "uniref90_id": "UniRef90_P47663", "gene": "rpsO", "product": "30S ribosomal protein S15", "uniref50_id": "UniRef50_P47663", "cog_id": "COG0184", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P47663", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47663" ], "product": "Small ribosomal subunit protein uS15" }, "genes": [ "rpsO" ], "id": "KMADJIBOEG_968", "locus": "GCA000292445_00968" }, { "type": "cds", "sequence": "contig_1", "start": 531106, "stop": 531234, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACTCAACCTTTCATGAGCTTGGGATCTCTCAAACTTTAATTGAAACGCTTAATGCGCTTCATATTAATAAGCCAACAAAAATTCAACAAATCTCTATCCCTCAGTTTTTATCAGAAAAAACTTAA", "aa": "MDSTFHELGISQTLIETLNALHINKPTKIQQISIPQFLSEKT", "aa_hexdigest": "1f0d770f4307e95b1587673d3c4617ff", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4766.427699999999, "isoelectric_point": 5.988863182067871 }, "id": "KMADJIBOEG_969", "locus": "GCA000292445_00969" }, { "type": "cds", "sequence": "contig_1", "start": 531492, "stop": 532439, "strand": "+", "frame": 3, "gene": "srmB", "product": "putative ATP-dependent RNA helicase MG425", "db_xrefs": [ "COG:COG0513", "COG:L", "EC:3.6.4.13", "GO:0003689", "GO:0003724", "GO:0005524", "GO:0005829", "GO:0009409", "GO:0016887", "GO:0033592", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "SO:0001217", "UniRef:UniRef50_P47664", "UniRef:UniRef90_P47664" ], "nt": "ATGGGTAGAATTATGGATCTTTTAGAGCGTAAAGCAATTCATTTTAGCGATTTAGAACACCTAATTATTGATGAAGTTGATTTAATGTTAGACCGTGGTTTTAAAAAACAAATTTTTAATTTACTAGAACAAATCAATTCCTTTAAACAAATTGCTGTTTATTCAGCTAGTTACAACCAAGAAGCTATTAACATTGCCAAGCAAATTACTAATAATGGGATCTTTATTGGATCACCTGAATTTAATAAAGACGCAAATACCAATAATGATAAACTAATCAACCAATTTGTTTGTTATCTATTTTCAGATCAAAAAAAGCAAGCTTTATACAGCCTTATAAAAACAGCACAAGTTAAGTCAATCATTGTTTTTTGTGACACTAAAAAACTAGTTGATGATCTTCATGTATTTTTAAGAAAAAATGAATTAAGAACTTTTGCACTTCATGGTGATAAAAAACAATTTATTAGAGAGAGAAATCTTAAAATCTTTGCCAATACAAAACAACCCACGATTCTAGTAACTACTGATCTTATTGGTCGTGGTATCCATGTTGAAGCAATCGATATGGTTATCAATTATTCAGCTTGTTTAAATCTAGAAGCTTATATAAATAGAATGGGAAGGACTGGCAGAAACAATCATAAAGGGACATGTGTAACTTTCTGCACCTCACAAGAAAAGAAAGTCTTTCTGAAAATGGTTGAGAAAATCACTGATAATCGAATAGCTGAATGTAAACAAATGGAAATAAAGTTAATTCCTTTAAAAAATAAAGCTAAAACTAAAAAAGGTGGTATTTCACTTGATTGTGTTCAGAAAATATATGCCAATGCAAAACCATATGACCGTAATAAACGTGTCCCTTTAGCAAGTGATCTTTTCAAAAGTCGTATGCGCCAGCCTGAAAAAGCTATGCAAAAGCAAAAAATTCATGACAATGACTGA", "aa": "MGRIMDLLERKAIHFSDLEHLIIDEVDLMLDRGFKKQIFNLLEQINSFKQIAVYSASYNQEAINIAKQITNNGIFIGSPEFNKDANTNNDKLINQFVCYLFSDQKKQALYSLIKTAQVKSIIVFCDTKKLVDDLHVFLRKNELRTFALHGDKKQFIRERNLKIFANTKQPTILVTTDLIGRGIHVEAIDMVINYSACLNLEAYINRMGRTGRNNHKGTCVTFCTSQEKKVFLKMVEKITDNRIAECKQMEIKLIPLKNKAKTKKGGISLDCVQKIYANAKPYDRNKRVPLASDLFKSRMRQPEKAMQKQKIHDND", "aa_hexdigest": "b90610c314dca6c62e3cae8cdd9cf868", "start_type": "ATG", "rbs_motif": null, "genes": [ "srmB" ], "truncated": "both", "pseudogene": { "start": 531193, "stop": 532454, "observations": { "deletion": [ 531226 ], "stop": [ 531442, 532438 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47664", "query_cov": 1.0, "subject_cov": 0.7015590200445434, "identity": 0.997, "score": 616.0, "evalue": 1.04e-220, "gene_start": 1, "gene_end": 315, "reference_start": 130, "reference_end": 444, "reference_sequence": "MDSTFHELGISQTLIETLNALHINKPTKIQQISIPQFLSEKNLIVHSPTGTGKTAAFAIPIIEKLLKEDQTAKPTLVIAPTRELVEQIKTTFSNIAKNKKLRIISLIGGVPAWKQIKKIKTNPQIIVGTMGRIMDLLERKAIHFSDLEHLIIDEVDLMLDRGFKKQIFNLLEQINSFKQIAVYSASYNQEAINIAKQITNNGIFIGSPEFNKDANTNNDKLIKQFVCYLFSDQKKQALYSLIKTAQVKSIIVFCDTKKLVDDLHVFLRKNELRTFALHGDKKQFIRERNLKIFANTKQPTILVTTDLIGRGIHVEAIDMVINYSACLNLEAYINRMGRTGRNNHKGTCVTFCTSQEKKVFLKMVEKITDNRIAECKQMEIKLIPLKNKAKTKKGGISLDCVQKIYANAKPYDRNKRVPLASDLFKSRMRQPEKAMQKQKIHDNDWQSNM" }, "paralog": false, "identity": 0.9928571428571429, "score": 810.0, "evalue": 9.29e-300, "description": "Internal stop codon at 531442, 532438. Frameshift due to deletion around 531226.", "psc": { "uniref90_id": "UniRef90_P47664", "gene": "SrmB", "product": "Probable ATP-dependent RNA helicase MG425", "ec_ids": [ "3.6.4.13" ], "uniref50_id": "UniRef50_P47664", "cog_id": "COG0513", "cog_category": "L", "go_ids": [ "GO:0005829", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003689", "GO:0003724", "GO:0033592", "GO:0009409" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47664", "EC:3.6.4.13", "UniRef:UniRef50_P47664", "COG:COG0513", "COG:L", "GO:0005829", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003689", "GO:0003724", "GO:0033592", "GO:0009409" ] }, "pscc": { "uniref50_id": "UniRef50_P47664", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47664" ], "product": "Probable ATP-dependent RNA helicase MG425" } }, "id": "KMADJIBOEG_970", "locus": "GCA000292445_00970" }, { "type": "cds", "sequence": "contig_1", "start": 532477, "stop": 532671, "strand": "+", "frame": 1, "gene": "rpmB", "product": "50S ribosomal protein L28", "db_xrefs": [ "COG:COG0227", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_009885606.1", "SO:0001217", "UniParc:UPI0000133E3F", "UniRef:UniRef100_P47665", "UniRef:UniRef50_Q6MTC5", "UniRef:UniRef90_P47665" ], "nt": "ATGGCAAAAAAAGACCAACTTACCTTAAGAGGGCCTTTGTATGGCAATAATCGTTCTCATTCCAAAACTATTACAAGAAGAAAATGGAATGTAAACCTTCAGTCATGCAAAATTAAAGATACTAATGGTAAAGTAACACGGATTCTAGTTTCAACTAAAACAATTCGTACCCTTAAAAAACAAAATCGTTTCTAA", "aa": "MAKKDQLTLRGPLYGNNRSHSKTITRRKWNVNLQSCKIKDTNGKVTRILVSTKTIRTLKKQNRF", "aa_hexdigest": "e60ec7143344c99d1a5fc9f7fa1f4c04", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133E3F", "ncbi_nrp_id": "WP_009885606.1", "uniref100_id": "UniRef100_P47665", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133E3F", "RefSeq:WP_009885606.1", "UniRef:UniRef100_P47665" ] }, "ips": { "uniref100_id": "UniRef100_P47665", "uniref90_id": "UniRef90_P47665" }, "psc": { "uniref90_id": "UniRef90_P47665", "gene": "rpmB", "product": "50S ribosomal protein L28", "uniref50_id": "UniRef50_Q6MTC5", "cog_id": "COG0227", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q6MTC5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MTC5" ], "product": "Large ribosomal subunit protein bL28" }, "genes": [ "rpmB" ], "id": "KMADJIBOEG_971", "locus": "GCA000292445_00971" }, { "type": "cds", "sequence": "contig_1", "start": 532707, "stop": 532997, "strand": "+", "frame": 3, "gene": "yhfA", "product": "Hydroperoxide reductase", "db_xrefs": [ "COG:COG1765", "COG:R", "EC:1.11.1.-", "GO:0004601", "GO:0005737", "SO:0001217", "UniRef:UniRef50_P47666", "UniRef:UniRef90_P47666" ], "nt": "ATGGATAAAAAATACGATATCACAGCTGTTTTGAACGATGATAGTTCTATTAATGCAGTAAGCGATAACTTCCAAATAACACTGGATGCACGACCAAAAGAAAAATCTAAAGGCATTAATCCTCTGTCAGCTTTTTTGGCTGGTTTAGCTGCATGTGAACTTGCAACTGCTAATGCGATGGCAGCTGCTAAGATGATAACTTTGAACAAGGCACTGATTAACATCAAAGGTTATCGTTTAACAAATCCTAGTGATGGTTATTTTGGCCTGCGTGAACTTAACATTCACTGA", "aa": "MDKKYDITAVLNDDSSINAVSDNFQITLDARPKEKSKGINPLSAFLAGLAACELATANAMAAAKMITLNKALINIKGYRLTNPSDGYFGLRELNIH", "aa_hexdigest": "e50dd3359b8c28aa987740bc630a4faf", "start_type": "ATG", "rbs_motif": null, "genes": [ "yhfA" ], "truncated": "3-prime", "pseudogene": { "start": 532707, "stop": 533132, "observations": { "mutation": [ 532995 ], "stop": [ 532995 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47666", "query_cov": 1.0, "subject_cov": 0.6808510638297872, "identity": 1.0, "score": 185.0, "evalue": 1.09e-58, "gene_start": 1, "gene_end": 96, "reference_start": 1, "reference_end": 96, "reference_sequence": "MDKKYDITAVLNDDSSINAVSDNFQITLDARPKEKSKGINPLSAFLAGLAACELATANAMAAAKMITLNKALINIKGYRLTNPSDGYFGLRELNIHWEIHSPNEEEEIKEFIDFVSKRCPAHNTLHGTSNFKINISVTLVH" }, "paralog": false, "identity": 0.9929078014184397, "score": 278.0, "evalue": 4.25e-98, "description": "Internal stop codon at 532995. Nonsense mutation around 532995.", "psc": { "uniref90_id": "UniRef90_P47666", "gene": "YhfA", "product": "Hydroperoxide reductase", "ec_ids": [ "1.11.1.-" ], "uniref50_id": "UniRef50_P47666", "cog_id": "COG1765", "cog_category": "R", "go_ids": [ "GO:0005737", "GO:0004601" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47666", "EC:1.11.1.-", "UniRef:UniRef50_P47666", "COG:COG1765", "COG:R", "GO:0005737", "GO:0004601" ] }, "pscc": { "uniref50_id": "UniRef50_P47666", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47666" ], "product": "Hydroperoxide reductase" } }, "id": "KMADJIBOEG_972", "locus": "GCA000292445_00972" }, { "type": "cds", "sequence": "contig_1", "start": 533461, "stop": 533757, "strand": "+", "frame": 1, "gene": "gerE", "product": "putative protein MG428", "db_xrefs": [ "COG:COG5905", "COG:DK", "GO:0003700", "GO:0006352", "SO:0001217", "UniRef:UniRef50_P47667", "UniRef:UniRef90_P47667" ], "nt": "ATGGTGAATAAAGTTGAATTGCTTGATTCTAAAAGTAAATACTCATTAGAAAAGCAAAACACAGTTCTAAATACACCTGAAAACTATTTACGGAGTTTAGAATTTAAAGAAATTATTACTAAAGCTTTTAATAAAGCTAAAAACGATCAAGAGAGAAAAGTTTTTTCTTTATATGTAAAAGGCTATAAAAACTTTGAGATAGCAAAAAAGCTCAATATTAGTCCTAGAAGAGTAAGATATTTATTAGATCTTTTTAAAAGCTACATCAAATTGTTAACAGAAAGATATGGATATTAA", "aa": "MVNKVELLDSKSKYSLEKQNTVLNTPENYLRSLEFKEIITKAFNKAKNDQERKVFSLYVKGYKNFEIAKKLNISPRRVRYLLDLFKSYIKLLTERYGY", "aa_hexdigest": "a60561533f3a89e19fe4e179790c3501", "start_type": "ATG", "rbs_motif": null, "genes": [ "gerE" ], "truncated": "5-prime", "pseudogene": { "start": 533242, "stop": 533757, "observations": { "mutation": [ 533296, 533305 ], "stop": [ 533296, 533305 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47667", "query_cov": 1.0, "subject_cov": 0.5730994152046783, "identity": 1.0, "score": 188.0, "evalue": 1.88e-59, "gene_start": 1, "gene_end": 98, "reference_start": 74, "reference_end": 171, "reference_sequence": "MKNNISDVKLGLLAAKIYWKSWRFLELTEDDIISIALHAEQDSKKRFNPEFGLSFDNYLKLNGANFIRSSFRSMVNKVELLDSKSKYSLEKQNTVLNTPENYLRSLEFKEIITKAFNKAKNDQERKVFSLYVKGYKNFEIAKKLNISPRRVRYLLDLFKSYIKLLTERYGY" }, "paralog": false, "identity": 0.9883040935672515, "score": 328.0, "evalue": 3.14e-117, "description": "Internal stop codon at 533296, 533305. Nonsense mutation around 533296, 533305.", "psc": { "uniref90_id": "UniRef90_P47667", "gene": "GerE", "product": "Uncharacterized protein MG428", "uniref50_id": "UniRef50_P47667", "cog_id": "COG5905", "cog_category": "DK", "go_ids": [ "GO:0003700", "GO:0006352" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47667", "UniRef:UniRef50_P47667", "COG:COG5905", "COG:DK", "GO:0003700", "GO:0006352" ] }, "pscc": { "uniref50_id": "UniRef50_P47667", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47667" ], "product": "Uncharacterized protein MG428" } }, "id": "KMADJIBOEG_973", "locus": "GCA000292445_00973" }, { "type": "cds", "sequence": "contig_1", "start": 533754, "stop": 533984, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02896.24" ], "nt": "ATGTGTGGTGAAATGGCCAGTGATCAATATGCAATACCATTGCTTTTAGGTTTAGGGTTAACAGAGTTATCAATGAGTGCAAGTTCAATGTTTAAAGCAAGAATGGTTATTGCTAAAATAACAATTAATGAATGTAAATCATTAGTTGAAAAAGCATTGAAATTAACTAGTGATAGTGCTGTTAGAAAACTAGTAGAAAATTTCTTTAAAAAGAAAAATATCTTTATTTAA", "aa": "MCGEMASDQYAIPLLLGLGLTELSMSASSMFKARMVIAKITINECKSLVEKALKLTSDSAVRKLVENFFKKKNIFI", "aa_hexdigest": "7507b1111836ac45260a9c1b52b55ee4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02896.24", "name": "PEP-utilizers_C", "length": 40, "aa_cov": 0.5263157894736842, "hmm_cov": 1.0, "evalue": 3.8612166282700225e-11, "score": 37.77103805541992, "start": 1, "stop": 40 } ], "seq_stats": { "molecular_weight": 8417.043499999992, "isoelectric_point": 9.301256752014158 }, "id": "KMADJIBOEG_974", "locus": "GCA000292445_00974" }, { "type": "cds", "sequence": "contig_1", "start": 533991, "stop": 534737, "strand": "-", "frame": 2, "gene": null, "product": "Phosphoenolpyruvate--protein phosphotransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAW9KJJ7" ], "nt": "TTGGAATCCAACTTTCAAAATGAATTAAAACAATATACCAATAAATTAGTAAAAACCTTAGATGGATATGAAGTTATTGTCGCTTCTAATATTGGTAATGTAAAAGATATGGATTTAGCAGTTGAGTATAACACGAATGGAATTGGTCTTTTTAGAACTGAATTTCTCTATATGAGTAGTCAAGATTGGCCTGATGAATCAGTTCAATTTGAAGCTTATAAAACTGTTTTACAAAAAGCTAAAAATGATTTGGTAATAATTAGAACATTAGACATTGGTGGAGATAAAAAACTAAATTATTTTCAATTTCCTCATGAAGATAACCCTTTCTTAGGTTATCGTGCTATCCGTTTAACATTGGATAAACAAGCTGTTTTTAAGACCCAACTTCGTGCTTTGTTAAGAGCTTCTGATTATGGAAATTTAGGAATTATGTTTCCAATGGTTGCAACTCTTGATGAATTAGTTCAAGTTAAGCAACTTTTAACAAAAGTTCAACAAGAATTTAATGAAACTAAGAAATTTAAATTAGGAATAATGATTGAAATTCCATCAGCAGCATTAGCAGCTGATTGCTTAGGAAAACATGTTGATTTTTTCTCAATAGGTAGCAATGATTTAATCCAATATAGTTTTGCTGCTGATAGGATGAATAAAAACGTTAGTTATCTATACCAACCATTAAATCCAGCATTACTACGCTTAATTAAACTAGTTGTAGAAGGTGGTAAATTAAATAATGTTTGA", "aa": "MESNFQNELKQYTNKLVKTLDGYEVIVASNIGNVKDMDLAVEYNTNGIGLFRTEFLYMSSQDWPDESVQFEAYKTVLQKAKNDLVIIRTLDIGGDKKLNYFQFPHEDNPFLGYRAIRLTLDKQAVFKTQLRALLRASDYGNLGIMFPMVATLDELVQVKQLLTKVQQEFNETKKFKLGIMIEIPSAALAADCLGKHVDFFSIGSNDLIQYSFAADRMNKNVSYLYQPLNPALLRLIKLVVEGGKLNNV", "aa_hexdigest": "ff7fbff54b2ceb4c13eec3aaaf3aa87d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002ACDB7C1", "query_cov": 0.8991935483870968, "subject_cov": 0.892, "identity": 0.525, "score": 219.0, "evalue": 2.63e-68, "valid": false, "product": "phosphoenolpyruvate--protein phosphotransferase", "uniref50_id": "UniRef50_A0AAW9KJJ7" }, "pscc": { "uniref50_id": "UniRef50_A0AAW9KJJ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAW9KJJ7" ], "product": "Phosphoenolpyruvate--protein phosphotransferase" }, "genes": [], "id": "KMADJIBOEG_975", "locus": "GCA000292445_00975" }, { "type": "cds", "sequence": "contig_1", "start": 534753, "stop": 535472, "strand": "-", "frame": 2, "gene": "ptsI", "product": "Phosphoenolpyruvate-protein phosphotransferase", "db_xrefs": [ "COG:COG1080", "COG:G", "EC:2.7.3.9", "GO:0005737", "GO:0008965", "GO:0009401", "GO:0016301", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47668", "UniRef:UniRef90_P47668" ], "nt": "ATGAAAAAAATAATTGGGATTGGTGTTTCAGATGGCATTGCTGTTGCAAAAGCTTTCATTATTCAAACACCTCAATTTGACGTTAAAAAATACACAAATGTCAAATTGACACCCACCCAAGCAAAAAAGCTTTTAAGCAGTGCTTTTCAAAAAGCAAAAAAAGACCTTGAAGAGATTAAGACAATTACTGTTAAGAATATTAATCAAGAAGCAGGAATGATTTTTGATGCACACATCCAAATTCTTAATGATCCTACTATCACTGAGCAATTAGAACAACAACTAAATAAAAACATCCATCCTGTTATTGCTGTTGATAATGTTTTTCAACAAACAGCTTTAATGTTTAGTGAAATGGATGATAAGTATTTTAAAGAACGTGCCAGTGATATATTAGATTTACACCAAAGGCTCTTAAGTTATTTAACTGGAGTAAAACTTAATGATTTAATCAGGATTAAAAGTGATGTCATTATAGTAGCTAATGATTTAACACCAAGTCAAACAGCAACTCTTAATAAAAAGTATGTCAAGGGTTTTTTAACTGAGAGTGGTGGCAGAACAAGTCATGCTGCCATTATGGCTCGTTCTATGGAAATTCCTGCCATAGTTGGTTTAAAAAATATTACCAGTAAAGTTGAAGATGGCAAAACAGTTGGTATCAATGGTAGAAAAGGTATTGTGGGTTTTGACTTTTCAAGTAAAGACATAACTCAATGA", "aa": "MKKIIGIGVSDGIAVAKAFIIQTPQFDVKKYTNVKLTPTQAKKLLSSAFQKAKKDLEEIKTITVKNINQEAGMIFDAHIQILNDPTITEQLEQQLNKNIHPVIAVDNVFQQTALMFSEMDDKYFKERASDILDLHQRLLSYLTGVKLNDLIRIKSDVIIVANDLTPSQTATLNKKYVKGFLTESGGRTSHAAIMARSMEIPAIVGLKNITSKVEDGKTVGINGRKGIVGFDFSSKDITQ", "aa_hexdigest": "7e0cbe148a72ca471e74b544b19da305", "start_type": "ATG", "rbs_motif": null, "genes": [ "ptsI" ], "truncated": "5-prime", "pseudogene": { "start": 534450, "stop": 535472, "observations": { "mutation": [ 534755 ], "stop": [ 534755 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47668", "query_cov": 1.0, "subject_cov": 0.4178321678321678, "identity": 0.992, "score": 454.0, "evalue": 2.18e-156, "gene_start": 1, "gene_end": 239, "reference_start": 1, "reference_end": 239, "reference_sequence": "MKKIIGIGVSDGIAVAKAFIIQTPQFDVKKYTNVKMTPTQAKKLLSSAFQKAKKDLEEIKTITVKNINQEAGMIFDAHIQILNDPTITEQLEQQLNKNIHPVIAVDNVFQQTALMFSEMDDKYFKERASDILDLHQRLLSYLTGVKLNDLIRIKSDVIIVANDLTPSQTATLNKKYVKGFLTESGGKTSHAAIMARSMEIPAIVGLKNITSKVEDGKTVGINGRKGIVGFDFSSKDITQWKQEKELESNFQNELKQYTNKLVKTLDGYEVIVASNIGNVKDMDLAVEYNTNGIGLFRTEFLYMSSQDWPDESVQFEAYKTVLQKAKNDLVIIRTLDIGGDKKLNYFQFPHEDNPFLGYRAIRLTLDKQAVFKTQLRALLRASDYGNLGIMFPMVATLDELVQVKQLLTKVQQEFNETKKFKLGIMIEIPSAALAADCLGKHVDFFSIGSNDLIQYSFAADRMNKNVSYLYQPLNPALLRLIKLVVEGGKLNNVWTGMCGEMASDQYAIPLLLGLGLTELSMSASSMFKARMVIAKITINECKSLVEKALKLTSDSAVRKLVENFFKKKNIFI" }, "paralog": false, "identity": 0.9911764705882353, "score": 654.0, "evalue": 1.79e-237, "description": "Internal stop codon at 534755. Nonsense mutation around 534755.", "psc": { "uniref90_id": "UniRef90_P47668", "gene": "ptsI", "product": "Phosphoenolpyruvate-protein phosphotransferase", "ec_ids": [ "2.7.3.9" ], "uniref50_id": "UniRef50_P47668", "cog_id": "COG1080", "cog_category": "G", "go_ids": [ "GO:0005737", "GO:0016301", "GO:0046872", "GO:0008965", "GO:0009401" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47668", "EC:2.7.3.9", "UniRef:UniRef50_P47668", "COG:COG1080", "COG:G", "GO:0005737", "GO:0016301", "GO:0046872", "GO:0008965", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P47668", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47668" ], "product": "Phosphoenolpyruvate-protein phosphotransferase" } }, "id": "KMADJIBOEG_976", "locus": "GCA000292445_00976" }, { "type": "cds", "sequence": "contig_1", "start": 535476, "stop": 536999, "strand": "-", "frame": 2, "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "db_xrefs": [ "COG:COG0696", "COG:G", "EC:5.4.2.12", "GO:0005829", "GO:0005975", "GO:0006007", "GO:0006096", "GO:0030145", "GO:0046537", "KEGG:K15633", "RefSeq:WP_014894337.1", "SO:0001217", "UniParc:UPI00027B42C1", "UniRef:UniRef100_UPI00027B42C1", "UniRef:UniRef50_P75167", "UniRef:UniRef90_P47669" ], "nt": "ATGCATAAAAAAGTTTTATTAGCAATCCTTGATGGTTATGGGATCTCAAATGCTATTTATGGTAATGCAGTACAAAATGCAAATACCCCAATGCTAGATGAATTAATCAATTCATATCCTTGTGTACTTTTAGATGCATCTGGGGAAGCAGTTGGATTGCCTATGGGTCAAATAGGTAACTCTGAGGTAGGTCATCTAAATATTGGGGCAGGTCGAGTTGTTTATACTGGACTTTCTTTGATTAATCAACATATTAAGGATCGTAGTTTTTTTGCAAATAAAGCTTTTTTAAAAACCATAGAACATGTAGAAAAAAACCATTCAAAAATCCATTTAATTGGGTTATTTTCCAATGGAGGAGTGCATAGTCATAATGAACATCTATTAGCACTCATTGAATTGTTTTCAAAACATGCAAAGGTAGTATTACATTTATTTGGTGATGGTAGAGATGTAGCACCTTGTAGCTTAAAACAAGATCTTGAGAAATTAATGATATTTCTAAAAAACTATCCTAATGTTGTTATTGGAACTATTGGGGGAAGATACTATGGAATGGATCGTGATCAACGCTGGGATCGTGAAATTATTGCTTATAAAGCTTTATTAGGAGTTTCAAAAAATAAATTCAATGACCCAATTGGTTATATTGAAACGCAATATCAGAACCAAATTACTGATGAATTTATTTATCCTGCAATTAATGCCAATTTAAATTCTGATCAGTTTGCATTAAACAATAATGATGGAGTTATTTTCTTTAATTTTAGACCTGATAGAGCAAGACAAATGTCCCATTTGATCTTTAACAGCAATTATTACAACTATCAACCTGAATTGAAACGAAAAGAAAATTTATTTTTTGTAACAATGATGAATTATGAGGGAATTGTACCTAGCGAAGTTGCTTTTCCACCTCAAACCATTAAAAATAGTCTTGGTGAAGTAATTGCTAATAATAATTTGAAGCAATTGAGGATTGCAGAAACTGAAAAGTATGCTCACGTTACTTTCTTTTTTGATGGTGGTTTTGAAGTTAATCTCAGCAATGAAACAAAGACATTAATTCCTTCTTTAAAAGTTGTTACATATGATTTAGCTCCCGAAATGTCATGTAAAGCTATTACTGATGCACTACTAGAAAAGCTTAATAACTTTGATTTTACTGTTTTAAATTTTGCTAATCCTGATATGGTAGGTCATACTGGTAACTATCAAGCTTGCATTAAAGCTCTTGAAGCACTCGATGTTCAAATTAAACGAATAGTTGATTTTTGTAAAGCTAATCAAATAACTATGTTTTTAACTGCAGATCATGGGAATGCAGAAGTGATGATTGATAATAATAACAATCCAGTTACTAAACACACTATTAATCCTGTACCATTTGTATGTACTGACAAAAATGTTAACTTTAATCAAACTGGAATTTTAGCTAATATTGCTCCTACTATCTTGGAATATCTTAACCTTAGCAAACCAAAAGAGATGACTGCAAAATCCTTATTAAAAAATAACAATTAA", "aa": "MHKKVLLAILDGYGISNAIYGNAVQNANTPMLDELINSYPCVLLDASGEAVGLPMGQIGNSEVGHLNIGAGRVVYTGLSLINQHIKDRSFFANKAFLKTIEHVEKNHSKIHLIGLFSNGGVHSHNEHLLALIELFSKHAKVVLHLFGDGRDVAPCSLKQDLEKLMIFLKNYPNVVIGTIGGRYYGMDRDQRWDREIIAYKALLGVSKNKFNDPIGYIETQYQNQITDEFIYPAINANLNSDQFALNNNDGVIFFNFRPDRARQMSHLIFNSNYYNYQPELKRKENLFFVTMMNYEGIVPSEVAFPPQTIKNSLGEVIANNNLKQLRIAETEKYAHVTFFFDGGFEVNLSNETKTLIPSLKVVTYDLAPEMSCKAITDALLEKLNNFDFTVLNFANPDMVGHTGNYQACIKALEALDVQIKRIVDFCKANQITMFLTADHGNAEVMIDNNNNPVTKHTINPVPFVCTDKNVNFNQTGILANIAPTILEYLNLSKPKEMTAKSLLKNNN", "aa_hexdigest": "87e0aa46a84d14504f3bea70c41ecc1b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B42C1", "ncbi_nrp_id": "WP_014894337.1", "uniref100_id": "UniRef100_UPI00027B42C1", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B42C1", "RefSeq:WP_014894337.1", "UniRef:UniRef100_UPI00027B42C1" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B42C1", "uniref90_id": "UniRef90_P47669" }, "psc": { "uniref90_id": "UniRef90_P47669", "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "ec_ids": [ "5.4.2.12" ], "uniref50_id": "UniRef50_P75167", "cog_id": "COG0696", "cog_category": "G", "kegg_orthology_id": "K15633", "go_ids": [ "GO:0005829", "GO:0005975", "GO:0006007", "GO:0006096", "GO:0030145", "GO:0046537" ] }, "pscc": { "uniref50_id": "UniRef50_P75167", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75167" ], "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase" }, "genes": [ "gpmI" ], "id": "KMADJIBOEG_977", "locus": "GCA000292445_00977" }, { "type": "cds", "sequence": "contig_1", "start": 537238, "stop": 537726, "strand": "-", "frame": 1, "gene": "tpiA", "product": "triose-phosphate isomerase", "db_xrefs": [ "COG:COG0149", "COG:G", "EC:5.3.1.1", "GO:0004807", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0019563", "GO:0046166", "KEGG:K01803", "SO:0001217", "UniRef:UniRef50_P78010", "UniRef:UniRef90_P47670" ], "nt": "ATGCGCACAAGGTATTTAATTGGCAATTGGAAAACAAATAAAAATTTAAAAGACGCAGTTAGTTTTGTTGAACAATTTCAACAAAATAAACTTAATTACAATGCCAAAATTGGGATAGCATCTGTTTATGTTCATCTCACTGAAATAAAAAAAATAATTAGTGATAGTCTCCTTTTATTTGCCCAAGACGCTAACTTTATTGAAAGTGGTTCATATACTGGAACTGTAAGCTTTACTCAACTTCAAGACATTGGTGTTAACAACAGTATTATTGGTCATTCTGAAAGAAGAAAATACTATAACGAAACCAGTGCAGTTATTAATCAAAAGCTCTTTGCTTGTCTAAAAGCATCCATGCAAGTAGTTTTATGTATTGGTGAGGCTTTAGGACAAGAGATTAGCTTTCTTAAAACTGATCTTACTAATTGCTTAAATACGATTGACAAAAGCTTAATTAAAAATTTAGTTATTGCTTATGAGCCTTTGTGA", "aa": "MRTRYLIGNWKTNKNLKDAVSFVEQFQQNKLNYNAKIGIASVYVHLTEIKKIISDSLLLFAQDANFIESGSYTGTVSFTQLQDIGVNNSIIGHSERRKYYNETSAVINQKLFACLKASMQVVLCIGEALGQEISFLKTDLTNCLNTIDKSLIKNLVIAYEPL", "aa_hexdigest": "85cd1228cdb891b0dd783036dd118562", "start_type": "ATG", "rbs_motif": null, "genes": [ "tpiA" ], "truncated": "5-prime", "pseudogene": { "start": 536992, "stop": 537726, "observations": { "mutation": [ 537240 ], "stop": [ 537240 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47670", "query_cov": 1.0, "subject_cov": 0.6639344262295082, "identity": 0.988, "score": 315.0, "evalue": 2.39e-107, "gene_start": 1, "gene_end": 162, "reference_start": 1, "reference_end": 162, "reference_sequence": "MRTRYLIGNWKTNKNLKDAVSFVEQFQQNKLNYNAKIGIAPVYVHLTEIKKIISDSLLLFAQDANFIESGSYTGTVSFTQLQDIGVNNSIIGHSERRKYYNETSAVINQKLFACLKASMQVVLCIGEALGQEISFLKTDLTNCLDTIDKSLIKNLVIAYEPLWAIGTGKTATPEVANQTIKTIREYINDLYDENVANNISILYGGSVDHNNIQKLAIMEQIDGFLVGKASLEIKNFLEMARVYA" }, "paralog": false, "identity": 0.9836065573770492, "score": 469.0, "evalue": 9.62e-171, "description": "Internal stop codon at 537240. Nonsense mutation around 537240.", "psc": { "uniref90_id": "UniRef90_P47670", "gene": "tpiA", "product": "triose-phosphate isomerase", "ec_ids": [ "5.3.1.1" ], "uniref50_id": "UniRef50_P78010", "cog_id": "COG0149", "cog_category": "G", "kegg_orthology_id": "K01803", "go_ids": [ "GO:0004807", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0019563", "GO:0046166" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47670", "EC:5.3.1.1", "UniRef:UniRef50_P78010", "COG:COG0149", "COG:G", "KEGG:K01803", "GO:0004807", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0019563", "GO:0046166" ] }, "pscc": { "uniref50_id": "UniRef50_P78010", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78010" ], "product": "Triosephosphate isomerase" } }, "id": "KMADJIBOEG_978", "locus": "GCA000292445_00978" }, { "type": "cds", "sequence": "contig_1", "start": 537786, "stop": 537998, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10035.14" ], "nt": "ATGTTTGAAGCTGAAGGAGGATATTCACGCCAAAAAACCCAAGTGCTTGTAACTAATTGTTTGCTTATTAAAGCTGCTAAGCTTTTAGAAGATGTGAGAAAGTTTGATCGTGATGCTTTGTTTTCCATTACTTTTATTAAAAAGCTTGATGGCTATATCTATGATAGACGTGCCAATAAGCAAACAAAACATGGAACTGAAAACAAGTCATAA", "aa": "MFEAEGGYSRQKTQVLVTNCLLIKAAKLLEDVRKFDRDALFSITFIKKLDGYIYDRRANKQTKHGTENKS", "aa_hexdigest": "68f12019e67831fc9cb12e3f9ddd37d1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10035.14", "name": "DUF2179", "length": 49, "aa_cov": 0.7, "hmm_cov": 1.0, "evalue": 2.8122922639621663e-09, "score": 31.878143310546875, "start": 1, "stop": 49 } ], "seq_stats": { "molecular_weight": 8113.248599999996, "isoelectric_point": 9.560741996765138 }, "id": "KMADJIBOEG_979", "locus": "GCA000292445_00979" }, { "type": "cds", "sequence": "contig_1", "start": 538011, "stop": 538286, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAATTAATACAGTTAGCTTTATTATTGGCTATGTAATTGGTACTTATTTAACTGGTAGTTTATCAGTGCAATCTTATGTTGGTGATGATAAACACCAGCCATTTGGAGTTGCTTTCTTTTTATCTCCAAACCTTGTTTTTACTCTATTGATGAACATTGTGTTGGGATTATTTACTTCTTTTTATTTCCCTAAGTATCAATTTGTAAAAGTAGAAGTTTATGGTAAGCACATAGAAAAAATTCGTAACTATCTACTTGATAATCAACAGTGA", "aa": "MLINTVSFIIGYVIGTYLTGSLSVQSYVGDDKHQPFGVAFFLSPNLVFTLLMNIVLGLFTSFYFPKYQFVKVEVYGKHIEKIRNYLLDNQQ", "aa_hexdigest": "a3d3a56c52259c9c7a9bcb3893067ea4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10451.071599999992, "isoelectric_point": 7.983909797668457 }, "id": "KMADJIBOEG_980", "locus": "GCA000292445_00980" }, { "type": "cds", "sequence": "contig_1", "start": 538458, "stop": 538790, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTCCATTAGCCAAGGATTAGCTCGTCTTGTTAATTTTTTTATTACTTCACAAAATATTAATGTTGATTCAGCAACTATATTTAATGCTATTTTTTGGTTAACACAAATTCTTTTTAATGTTCCTTTCTTTATTTTTGGATGGTTTAAAATCTCCAAAAAATTTACCCTATTAACCCTTTATTTTGTAGCTGTTTCAAATCTTTTTGGTTTTTTCTTTTCATACATCCCTGGAATAGATAACTTCTTTTTGTTTGCAAATTTAACTACAGCAAAAGATGGTGGATTTGAAAATTTAATTAATGAAAAGGGAGTACAACTTATATTTTGA", "aa": "MASISQGLARLVNFFITSQNINVDSATIFNAIFWLTQILFNVPFFIFGWFKISKKFTLLTLYFVAVSNLFGFFFSYIPGIDNFFLFANLTTAKDGGFENLINEKGVQLIF", "aa_hexdigest": "d07475790e5d554120dc696c3924d2e9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12564.50529999998, "isoelectric_point": 8.063850593566894 }, "id": "KMADJIBOEG_981", "locus": "GCA000292445_00981" }, { "type": "cds", "sequence": "contig_1", "start": 539068, "stop": 539184, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAACGAAAATAGAGCTAATAAAAGAATTGCGTAAATCAACACAAGCAAGTGTTATGGATTGTAAACAAGCTTTGGAAAAAAATAATGATGATTTTGAGAAAGCTGTTAAGTGA", "aa": "MATKIELIKELRKSTQASVMDCKQALEKNNDDFEKAVK", "aa_hexdigest": "a22b1c8f6cd0bba9263dc05eb1fb1408", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4355.0216, "isoelectric_point": 7.870574378967285 }, "id": "KMADJIBOEG_982", "locus": "GCA000292445_00982" }, { "type": "cds", "sequence": "contig_1", "start": 539281, "stop": 539673, "strand": "+", "frame": 1, "gene": "tsf", "product": "translation elongation factor Ts", "db_xrefs": [ "COG:COG0264", "COG:J", "GO:0003746", "GO:0005737", "GO:0006414", "SO:0001217", "UniRef:UniRef50_P47246", "UniRef:UniRef90_P47246" ], "nt": "ATGGTTGAGATAAACTCACAAACTGATTTTGTAGCCAAAAATCAAGAGTTAAAAGAATTTTCAGATTTAATGCTTGAAAAAATATTTGAAAAAGTAAATCCAAAAACAGAATTAGTTGAAATTGAAAAAATTCAAATTAATAATGATGAAAAAGTTAGTGAAAAACTAGCATTAATTGCTTCTAAAACTGATGAGAAAATAGTACTTAGAAGAGTAGTTGTATTTGAAACTAAAACTAATCAAATTTTCACCTATTTACATGCCAATAAAAGAATTGGGGTAATTATTGAGATTCAAGGAAAACTCAACGAAGATGATGGTAAGCATTTAGCAATGCATATTGCTGCTAATTCACCACAATTTATTGATCAAAGTGATGTTAATCAAACATGA", "aa": "MVEINSQTDFVAKNQELKEFSDLMLEKIFEKVNPKTELVEIEKIQINNDEKVSEKLALIASKTDEKIVLRRVVVFETKTNQIFTYLHANKRIGVIIEIQGKLNEDDGKHLAMHIAANSPQFIDQSDVNQT", "aa_hexdigest": "c5609d87f049217d1d811b231ae7842f", "start_type": "ATG", "rbs_motif": null, "genes": [ "tsf" ], "truncated": "both", "pseudogene": { "start": 539068, "stop": 539964, "observations": { "mutation": [ 539182, 539671 ], "stop": [ 539182, 539671 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47246", "query_cov": 1.0, "subject_cov": 0.436241610738255, "identity": 1.0, "score": 244.0, "evalue": 1.81e-79, "gene_start": 1, "gene_end": 130, "reference_start": 72, "reference_end": 201, "reference_sequence": "MATKIELIKELRKSTQASVMDCKQALEKNNDDFEKAVKWLRENGIVKSTKKLNKVASEGIIVLKSNLHKAIMVEINSQTDFVAKNQELKEFSDLMLEKIFEKVNPKTELVEIEKIQINNDEKVSEKLALIASKTDEKIVLRRVVVFETKTNQIFTYLHANKRIGVIIEIQGKLNEDDGKHLAMHIAANSPQFIDQSDVNQTWLQNERNIIRSQAELEVKENPKKAIFLEKTIEGRVNKLLIDTCLINQKYLIDETKTIGQFLKEKQAKVLKFIRYEVGEGIIKETVDFVSEVNAQIKQ" }, "paralog": false, "identity": 0.9932885906040269, "score": 562.0, "evalue": 3.94e-207, "description": "Internal stop codon at 539182, 539671. Nonsense mutation around 539182, 539671.", "psc": { "uniref90_id": "UniRef90_P47246", "gene": "tsf", "product": "translation elongation factor Ts", "uniref50_id": "UniRef50_P47246", "cog_id": "COG0264", "cog_category": "J", "go_ids": [ "GO:0003746", "GO:0005737", "GO:0006414" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47246", "UniRef:UniRef50_P47246", "COG:COG0264", "COG:J", "GO:0003746", "GO:0005737", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_P47246", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47246" ], "product": "Elongation factor Ts" } }, "id": "KMADJIBOEG_983", "locus": "GCA000292445_00983" }, { "type": "cds", "sequence": "contig_1", "start": 539945, "stop": 540676, "strand": "+", "frame": 2, "gene": "pyrH", "product": "UMP kinase", "db_xrefs": [ "COG:COG0528", "COG:F", "EC:2.7.4.22", "GO:0005524", "GO:0005737", "GO:0006221", "GO:0006225", "GO:0015949", "GO:0033862", "GO:0044210", "KEGG:K09903", "RefSeq:WP_014894340.1", "SO:0001217", "UniParc:UPI00027B3173", "UniRef:UniRef100_UPI00027B3173", "UniRef:UniRef50_P47672", "UniRef:UniRef90_P47672" ], "nt": "ATGCACAAATCAAACAATAAAATCCGCCAAAGAATAATCATTAAACTTAGTGGTGCTGGGCTAACCAAAGAAAATTCTCAACCCTTTTCTAATGATTTTTTTGAAACTATTATTAATCAATTAAAAGTTTTAAAAGAAAGCTATCAAGTAGGAATTGTTATTGGTGGGGGTAACATTATCAGAGGTAATAATTGCCAAGAATTTAACATTGCTGAATACCATGGTCATCAACTTGGTATTATAGCAACAGTAGTTAATGGCTATTTTTTAAAAGCAAAGTTAGATGCACATAATTTGAAAAGTGCTTTACTAAGTGCAATTAGTTGTCCTAGTTTAGCAGTGCAAATTCTTTCACAGCAAACTATTGATAAAGCTTTTGAAGAGAATGACTTTGTCATTTTTTCAGGTGGCACTGGTAATCCTTATTTTTCCACTGACACTGCATTAGCTTTAAGAGCAGTGCAAACAAAAGCAGTTGCTATTCTGATTGGAAAAAATGGTGTTGATGGTGTTTATACAGCTGATCCTAAAAAAGATAAAAATGCAACCTTTTTACCAACACTCAACTATGACCATGCCATTAAAAATGATTTGAAAATTATGGATATTACTGCTTTTACTATGTGTAAGGAAAATAATCTGAAAATAATTATTTTTAACATTAATACTGAGAATGCATTATTAGATGCATTAAACAAAAAAGGTCGCTTTACTATAATTGAAAATAACTAA", "aa": "MHKSNNKIRQRIIIKLSGAGLTKENSQPFSNDFFETIINQLKVLKESYQVGIVIGGGNIIRGNNCQEFNIAEYHGHQLGIIATVVNGYFLKAKLDAHNLKSALLSAISCPSLAVQILSQQTIDKAFEENDFVIFSGGTGNPYFSTDTALALRAVQTKAVAILIGKNGVDGVYTADPKKDKNATFLPTLNYDHAIKNDLKIMDITAFTMCKENNLKIIIFNINTENALLDALNKKGRFTIIENN", "aa_hexdigest": "37b6c2c104754f443479dbece991da00", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3173", "ncbi_nrp_id": "WP_014894340.1", "uniref100_id": "UniRef100_UPI00027B3173", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3173", "RefSeq:WP_014894340.1", "UniRef:UniRef100_UPI00027B3173" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3173", "uniref90_id": "UniRef90_P47672" }, "psc": { "uniref90_id": "UniRef90_P47672", "gene": "pyrH", "product": "UMP kinase", "ec_ids": [ "2.7.4.22" ], "uniref50_id": "UniRef50_P47672", "cog_id": "COG0528", "cog_category": "F", "kegg_orthology_id": "K09903", "go_ids": [ "GO:0005524", "GO:0005737", "GO:0006221", "GO:0006225", "GO:0015949", "GO:0033862", "GO:0044210" ] }, "pscc": { "uniref50_id": "UniRef50_P47672", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47672" ], "product": "Uridylate kinase" }, "genes": [ "pyrH" ], "id": "KMADJIBOEG_984", "locus": "GCA000292445_00984" }, { "type": "cds", "sequence": "contig_1", "start": 540799, "stop": 541227, "strand": "+", "frame": 1, "gene": "frr", "product": "ribosome recycling factor", "db_xrefs": [ "COG:COG0233", "COG:J", "GO:0005737", "GO:0006412", "GO:0006415", "GO:0008135", "GO:0043023", "SO:0001217", "UniRef:UniRef50_P47673", "UniRef:UniRef90_P47673" ], "nt": "TTGATTGAAAGTTATGGACAAAAAATGCCTTTAATATCTTTAGCTCAAGTGACTATTAATCCGCCAAGAGAAATAATCATAAAACCATTTGATCCTAAGAGTAATACTAATGCTATTTACAGTGAAATTCAGCGGGCAAACATTGGTGTTCAACCAGTTATTGATGGTGAAAAAATTCGTGTTAATTTTCCCCAAATTACTCAAGAAACTCGCTTAGAAAATATTAAGCACGTTAAAAAAATAATAGAGCAAATTTATCAAGAACTGAGGGTTGTAAGAAGAGATGCATTACAAATGATTAAAAAAGATAATCACAATGAGGATTTAGAAAACTCTTTAAAAGCTGAAATAGAAAAAATTAACAAAAATTATTCTAATCAATTAGAAGAGATTCAAAAAGACAAAGAAAAAGAATTGCTAACAATTTAA", "aa": "MIESYGQKMPLISLAQVTINPPREIIIKPFDPKSNTNAIYSEIQRANIGVQPVIDGEKIRVNFPQITQETRLENIKHVKKIIEQIYQELRVVRRDALQMIKKDNHNEDLENSLKAEIEKINKNYSNQLEEIQKDKEKELLTI", "aa_hexdigest": "1026a425a53f60a7b5e25902a53eb132", "start_type": "TTG", "rbs_motif": null, "genes": [ "frr" ], "truncated": "5-prime", "pseudogene": { "start": 540676, "stop": 541227, "observations": { "mutation": [ 540733 ], "stop": [ 540733 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47673", "query_cov": 1.0, "subject_cov": 0.7759562841530054, "identity": 0.993, "score": 264.0, "evalue": 1.68e-88, "gene_start": 1, "gene_end": 142, "reference_start": 42, "reference_end": 183, "reference_sequence": "MTKAHYIDFFKQAADKKIQWLKEELTKIRTGRPNPKIFDNLLIESYGQKMPLISLAQVTINPPREIIIKPFDPKSNTNAIYSEIQRANIGVQPVIDGEKIRVNFPQITQETRLENIKHVKKIIEQIYQELRVVRRDALQMIKKDNHNEDLENSLKAEIEKINKNYSNQLEEIQKDKEKELLTI" }, "paralog": false, "identity": 0.994535519125683, "score": 357.0, "evalue": 1.35e-127, "description": "Internal stop codon at 540733. Nonsense mutation around 540733.", "psc": { "uniref90_id": "UniRef90_P47673", "gene": "frr", "product": "ribosome recycling factor", "uniref50_id": "UniRef50_P47673", "cog_id": "COG0233", "cog_category": "J", "go_ids": [ "GO:0005737", "GO:0006412", "GO:0006415", "GO:0008135", "GO:0043023" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47673", "UniRef:UniRef50_P47673", "COG:COG0233", "COG:J", "GO:0005737", "GO:0006412", "GO:0006415", "GO:0008135", "GO:0043023" ] }, "pscc": { "uniref50_id": "UniRef50_P47673", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47673" ], "product": "Ribosome-recycling factor" } }, "id": "KMADJIBOEG_985", "locus": "GCA000292445_00985" }, { "type": "cds", "sequence": "contig_1", "start": 541224, "stop": 541529, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGTGAACAACTCTCAAATCATCAGAGAACCAATTACCCCAACAATTAAAATAATGATTATGCTAAAGCTGAAACGAACAGCTTTAAAGTTTTCATTAATCTCAGTACTAATAACACTAGTAACACCACTTGCTTGTTCTACTTTTAATGTTCTTGAATTGAAATCTGCTGATCACGGTGATCAAAAGTTAAAACCATCAGCAAATGCGCTAATTAAAAGAAAAAAGCAAAATACAACTAATAAAGCAATGAATACTGAAGTTCGCTTTTTGATGAATTGTTTTGCTTTTTCATTCATTTAA", "aa": "MFVNNSQIIREPITPTIKIMIMLKLKRTALKFSLISVLITLVTPLACSTFNVLELKSADHGDQKLKPSANALIKRKKQNTTNKAMNTEVRFLMNCFAFSFI", "aa_hexdigest": "1f5a7a4795e76c8d92c36f3edb1f81c2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11444.656099999982, "isoelectric_point": 10.24945774078369 }, "id": "KMADJIBOEG_986", "locus": "GCA000292445_00986" }, { "type": "cds", "sequence": "contig_1", "start": 541701, "stop": 541790, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAGCAATAAAGCAAGCAATAGCTCCTAAGCTATATGAAAGTTGTAAGCTAAACTTAATTCTTGTTTTACTCATGCAAGCAATGTAA", "aa": "MLAIKQAIAPKLYESCKLNLILVLLMQAM", "aa_hexdigest": "fa9b394f8ba216dce46217d0ad3627f1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3261.1224, "isoelectric_point": 9.102242851257323 }, "id": "KMADJIBOEG_987", "locus": "GCA000292445_00987" }, { "type": "cds", "sequence": "contig_1", "start": 541800, "stop": 541970, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGCTAATGATGCCAAAAAAAGCTCCTTCTCATGTTTTTGATGGTGAAATTTTAATTAAAGGATTTTTACCAAAGCACTTTCCAAAAAGAAAAGAAAATGTATCAACACTGACTGCTAAACTCATCAATAAAAAGATTGTAGTTCAACCCCTGATAACAGAAAAGTAA", "aa": "MVLMMPKKAPSHVFDGEILIKGFLPKHFPKRKENVSTLTAKLINKKIVVQPLITEK", "aa_hexdigest": "c7b8c5ee405e14fa46a1f974b7dc7441", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6383.7651000000005, "isoelectric_point": 10.173578453063964 }, "id": "KMADJIBOEG_988", "locus": "GCA000292445_00988" }, { "type": "cds", "sequence": "contig_1", "start": 542501, "stop": 543016, "strand": "+", "frame": 2, "gene": "hsdS", "product": "Putative type I specificity subunit S-MgeORF438P", "db_xrefs": [ "COG:COG0732", "COG:V", "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_Q49434", "UniRef:UniRef90_Q49434" ], "nt": "TTGTTTGATTTAAAAAAAGGTGAAATGCTTGAAAAAGAGTTAATTACACCTGAGGGAAAATATGAATATTTTAACGGTGGTGTAAAAAATTCAGGAAGAACTGACAAGTTCAATACTTTTAAGAACACTATTAGTGTAATTGTTGGTGGATCTTGTGGTTATGTAAGGCTAGCTGACAAAAATTTCTTTTGTGGTCAAAGTAATTGCACATTAAATTTGTTAGATCCGCTTGAACTTGATCTTAAATTTGCTTACTATGCTTTGAAATCACAACAAGAAAGAATAGAGGCTTTAGCTTTTGGGACCACGATTCAAAACATTAGAATTTCAGATTTAAAAGAATTGGAAATTCCTTTCACATCAAATAAGAATGAACAGCATGCTATTGCAAATACTTTAAGTGTTTTTGATGAGAGACTAGAAAACTTAGCTTCTTTAATTGAGATTAACAGGAAACTAAGAGATGAATATGCTCACAAACTCTTTAGCTTAGATGAAGCTTTTCTAAGTCATTGA", "aa": "MFDLKKGEMLEKELITPEGKYEYFNGGVKNSGRTDKFNTFKNTISVIVGGSCGYVRLADKNFFCGQSNCTLNLLDPLELDLKFAYYALKSQQERIEALAFGTTIQNIRISDLKELEIPFTSNKNEQHAIANTLSVFDERLENLASLIEINRKLRDEYAHKLFSLDEAFLSH", "aa_hexdigest": "e5fdeeacefa01ea5afc6057b9fd7a38f", "start_type": "TTG", "rbs_motif": null, "genes": [ "hsdS" ], "truncated": "both", "pseudogene": { "start": 542441, "stop": 543319, "observations": { "mutation": [ 542477, 543014 ], "stop": [ 542477, 543014 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49434", "query_cov": 1.0, "subject_cov": 0.4211822660098522, "identity": 0.9940000000000001, "score": 340.0, "evalue": 7.1e-115, "gene_start": 1, "gene_end": 171, "reference_start": 44, "reference_end": 214, "reference_sequence": "MGHHHHHHHHHHSSGHIDDDDKHMTPKLKLNNNINWTKRTIDSLFDLKKGEMLEKELITPEGKYEYFNGGVKNSGRTDKFNTFKNTISVIVGGSCGYVRLADKNFFCGQSNCTLNLLDPLELDLKFAYYALKSQQERIEALAFGTTIQNIRISDLKELEIPFTSNKNEQHAIANTLSVFDERLENLASLIEINRKLRDEYAHKLFSLDEAFLSHWKLEALQSQMHEITLGEIFNFKSGKYLKSEERLEEGKFPYYGAGIDNTGFVAEPNTEKDTISIISNGYSLGNIRYHEIPWFNGTGSIALEPMNNEIYVPFFYCALKYLQKDIKERMKSDDSPFLSLKLAGEIKVPYVKSFQLQRKAGKIVFLLDQKLDQYKKELSSLTVIRDTLLKKLFPDMTERTKSIKDY" }, "paralog": false, "identity": 0.9931506849315068, "score": 581.0, "evalue": 2.55e-212, "description": "Internal stop codon at 542477, 543014. Nonsense mutation around 542477, 543014.", "psc": { "uniref90_id": "UniRef90_Q49434", "gene": "HsdS", "product": "Putative type I specificity subunit S.MgeORF438P", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_Q49434", "cog_id": "COG0732", "cog_category": "V", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49434", "EC:3.1.21.3", "UniRef:UniRef50_Q49434", "COG:COG0732", "COG:V", "KEGG:K01154", "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_Q49434", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49434" ], "product": "Putative type I specificity subunit S.MgeORF438P" } }, "id": "KMADJIBOEG_989", "locus": "GCA000292445_00989" }, { "type": "cds", "sequence": "contig_1", "start": 543041, "stop": 543592, "strand": "+", "frame": 2, "gene": "hsdS", "product": "Putative type I specificity subunit S-MgeORF438P", "db_xrefs": [ "COG:COG0732", "COG:V", "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_Q49434", "UniRef:UniRef90_Q49434" ], "nt": "ATGCATGAAATTACTTTAGGGGAAATATTTAATTTCAAAAGTGGTAAATATCTAAAGAGCGAGGAGAGATTAGAAGAAGGGAAATTTCCTTATTATGGAGCGGGAATTGATAACACTGGTTTTGTAGCTGAACCTAATACTGAAAAAGACACAATTTCTATTATTTCTAATGGTTATTCACTAGGTAACATCAGGTATCATGAAATTCCTTGGTTTAATGGCACAGGTAGCATTGCATTAGAACCCATGAATAATGAAATCTATGTACCATTCTTTTACTGTGCTTTAAAGTATTTGCAAAAAGATATTAAAGAAAGAATGAAAAGTGATGATTCACCTTTTTTATCCTTAAAACTAGCAGGTGAAATTAAAGTGCCTTATGTTAAGTCATTTCAACTGCAAAGAAAGGCAGGAAAAATCGTCTTTTTGTTAGATCAAAAATTAGACCAATATAAAAAAGAACTAAGTTCTTTAACAGTGATTCGTGACACTTTGTTAAAAAAATTATTCCCTGATATGACTGAAAGAACTAAATCTATTAAGGATTATTAA", "aa": "MHEITLGEIFNFKSGKYLKSEERLEEGKFPYYGAGIDNTGFVAEPNTEKDTISIISNGYSLGNIRYHEIPWFNGTGSIALEPMNNEIYVPFFYCALKYLQKDIKERMKSDDSPFLSLKLAGEIKVPYVKSFQLQRKAGKIVFLLDQKLDQYKKELSSLTVIRDTLLKKLFPDMTERTKSIKDY", "aa_hexdigest": "7d31abd2f905c0cba3c8bd336bca395d", "start_type": "ATG", "rbs_motif": null, "genes": [ "hsdS" ], "truncated": "5-prime", "pseudogene": { "start": 542741, "stop": 543592, "observations": { "mutation": [ 543014 ], "stop": [ 543014 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49434", "query_cov": 1.0, "subject_cov": 0.45073891625615764, "identity": 1.0, "score": 368.0, "evalue": 9.67e-126, "gene_start": 1, "gene_end": 183, "reference_start": 224, "reference_end": 406, "reference_sequence": "MGHHHHHHHHHHSSGHIDDDDKHMTPKLKLNNNINWTKRTIDSLFDLKKGEMLEKELITPEGKYEYFNGGVKNSGRTDKFNTFKNTISVIVGGSCGYVRLADKNFFCGQSNCTLNLLDPLELDLKFAYYALKSQQERIEALAFGTTIQNIRISDLKELEIPFTSNKNEQHAIANTLSVFDERLENLASLIEINRKLRDEYAHKLFSLDEAFLSHWKLEALQSQMHEITLGEIFNFKSGKYLKSEERLEEGKFPYYGAGIDNTGFVAEPNTEKDTISIISNGYSLGNIRYHEIPWFNGTGSIALEPMNNEIYVPFFYCALKYLQKDIKERMKSDDSPFLSLKLAGEIKVPYVKSFQLQRKAGKIVFLLDQKLDQYKKELSSLTVIRDTLLKKLFPDMTERTKSIKDY" }, "paralog": false, "identity": 0.9964664310954063, "score": 556.0, "evalue": 2.22e-202, "description": "Internal stop codon at 543014. Nonsense mutation around 543014.", "psc": { "uniref90_id": "UniRef90_Q49434", "gene": "HsdS", "product": "Putative type I specificity subunit S.MgeORF438P", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_Q49434", "cog_id": "COG0732", "cog_category": "V", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49434", "EC:3.1.21.3", "UniRef:UniRef50_Q49434", "COG:COG0732", "COG:V", "KEGG:K01154", "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_Q49434", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49434" ], "product": "Putative type I specificity subunit S.MgeORF438P" } }, "id": "KMADJIBOEG_990", "locus": "GCA000292445_00990" }, { "type": "cds", "sequence": "contig_1", "start": 543997, "stop": 544350, "strand": "-", "frame": 1, "gene": null, "product": "putative lipoprotein MG439", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75150", "UniRef:UniRef90_P47677" ], "nt": "ATGACTGGTATAGTTGGTAGTGACAATACTAGTACTTCATTAATCAAATCCTTTTTAGCTAGTACAAGTGGTGTAAGTGAAAGTGATTTTACTGGTGATAAAGCTAATTTTGATGGTGATGTTATCTTTACTTATACTCCTCCAACAGATAATAGGAGGGTAAGTGAAAGTACTTTTAGTAGTATACCTGCTTCAATTAACTTTCCTTTTGATATCAAAATTGACATGAGTACTTCTCATGAAAAGTTGAATTTATTACTTTCAACAAATGAACAAGTGAAGAAAATTAGAACAAGAACTTTTAAGGGAAAGTCAATCGATCTTTTACCGTTTTTTTATTACACATTGCTTTAA", "aa": "MTGIVGSDNTSTSLIKSFLASTSGVSESDFTGDKANFDGDVIFTYTPPTDNRRVSESTFSSIPASINFPFDIKIDMSTSHEKLNLLLSTNEQVKKIRTRTFKGKSIDLLPFFYYTLL", "aa_hexdigest": "a03fffb2e085b6b3d2d4fdce297da6b4", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 543997, "stop": 544650, "observations": { "mutation": [ 544392, 544608 ], "stop": [ 544392, 544608 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47677", "query_cov": 1.0, "subject_cov": 0.43014705882352944, "identity": 1.0, "score": 226.0, "evalue": 6.37e-73, "gene_start": 1, "gene_end": 117, "reference_start": 156, "reference_end": 272, "reference_sequence": "MKLRKIFLLPLISLSTLSVACSSTDPGLEKAQAYFQRNNIELSKNNAVTFLKKGYSSDKEEVINTVFASWKTTLLDYQLLEKPLDYSRFAKAFGVNKSKEDVTPNISAKGLYFDETYPGISGQIALVLGVKSQKVVNFQYSWKNNLDFKVQIHLKMTGIVGSDNTSTSLIKSFLASTSGVSESDFTGDKANFDGDVIFTYTPPTDNRRVSESTFSSIPASINFPFDIKIDMSTSHEKLNLLLSTNEQVKKIRTRTFKGKSIDLLPFFYYTLL" }, "paralog": false, "identity": 0.9907834101382489, "score": 419.0, "evalue": 2.88e-151, "description": "Internal stop codon at 544392, 544608. Nonsense mutation around 544392, 544608.", "psc": { "uniref90_id": "UniRef90_P47677", "product": "Uncharacterized lipoprotein MG439", "uniref50_id": "UniRef50_P75150", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47677", "UniRef:UniRef50_P75150", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75150", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75150" ], "product": "Uncharacterized lipoprotein MG439 homolog 1" } }, "id": "KMADJIBOEG_991", "locus": "GCA000292445_00991" }, { "type": "cds", "sequence": "contig_1", "start": 544606, "stop": 544815, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGAAACTCAGAAAGATCTTTTTATTACCATTAATTTCACTTAGTACTTTATCTGTAGCCTGTTCATCAACTGATCCTGGTTTAGAAAAAGCACGAGCTTATTTTCAGAGAAACAATATAGAACTTTCAAAAAATAATGCTGTTACTTTTTTAAAAAAGGGTTATAGTTCTGATAAGGAAGAGGTAATTAATACTGTATTTGCCAGTTGA", "aa": "MKLRKIFLLPLISLSTLSVACSSTDPGLEKARAYFQRNNIELSKNNAVTFLKKGYSSDKEEVINTVFAS", "aa_hexdigest": "adda8cef89cbd3c8b1bd5b3eaed1f7a7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 39, "aa_cov": 0.5507246376811594, "hmm_cov": 1.0, "evalue": 7.156247279829182e-09, "score": 30.612537384033203, "start": 32, "stop": 69 } ], "seq_stats": { "molecular_weight": 7697.838599999997, "isoelectric_point": 9.516839027404785 }, "id": "KMADJIBOEG_992", "locus": "GCA000292445_00992" }, { "type": "cds", "sequence": "contig_1", "start": 544815, "stop": 545387, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MG440", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47678", "UniRef:UniRef90_P47678" ], "nt": "TTGCAAGACGAAAAGATTCTTGAAAAACAAGACCCAAGTAGATTTGTAAAAGCATTTGGCAGTGGTAAGAGTAAGGAAGATGTTGAACCAAGCACTGGTGTTAAAGGCTTACGTTTAGTTGAAAGATACACGCAAAATGTTGCAAGTATTATTAATAATGTAATTAAATTGAGTAGTCAAACAGTTACAGATTTTAGTTTTTACTATAACAGTTCTCGTGATTTCAAAGTCCAAATAAGGTTACAAGCTACTGGAACTTTTGACAGTGCTCAAGCAAAATCATATTTGAGTCAAATAGGATTAAGTGATAGTGATATAAAAGATAAAAATTCTATTTCAGCTGATTTAATTTTTACTTATACTCCACCTTCCAGTAATTTATTTTCCAAATCTAGTTTTGATACTCTATTGAGAAAAATTAATTTTAATACCAATTTAAGGTTACAAATTATTGGTAAAGATGAATTGATGCAAAAGTTATTACAAAGTAATTTTGTAGGTCAATTAGCTGATCAAAACTTCCAAGATCAAACTATCGATCTTTTACCATATGTGCTTTATTCCATTTTGTAA", "aa": "MQDEKILEKQDPSRFVKAFGSGKSKEDVEPSTGVKGLRLVERYTQNVASIINNVIKLSSQTVTDFSFYYNSSRDFKVQIRLQATGTFDSAQAKSYLSQIGLSDSDIKDKNSISADLIFTYTPPSSNLFSKSSFDTLLRKINFNTNLRLQIIGKDELMQKLLQSNFVGQLADQNFQDQTIDLLPYVLYSIL", "aa_hexdigest": "aa48a3e9d5860d5cfb0b2536a55cf1cc", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 544815, "stop": 545639, "observations": { "mutation": [ 545399, 545621 ], "stop": [ 545399, 545621 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47678", "query_cov": 1.0, "subject_cov": 0.6934306569343066, "identity": 0.995, "score": 359.0, "evalue": 4.59e-124, "gene_start": 1, "gene_end": 190, "reference_start": 85, "reference_end": 274, "reference_sequence": "MLKKQFWTKAGIISSLMVLGSLITACSNLSSGTNFFSLNQLRNSFSKNSELNQDKKELVTSLRDSFEVDSKSATNVLLDAWRFSLQDEKILEKQDPSRFVKAFGSGKSKEDVEPSTGVKGLRLVERYTQNVASIINNVIKLSSQTVTDFSFYYNSSRDFKVQIRLQATGTFDSAQAKSYLSQIGLSDSDIKDKNSISADLIFTYTPPSSNLFSKSSFDTLLRKINFNTNLRLQIIGKDELMQKLLQSNFVGQLADQNFQDQTIDLLPYVLYSIL" }, "paralog": false, "identity": 0.9890510948905109, "score": 514.0, "evalue": 1.85e-187, "description": "Internal stop codon at 545399, 545621. Nonsense mutation around 545399, 545621.", "psc": { "uniref90_id": "UniRef90_P47678", "product": "Uncharacterized protein MG440", "uniref50_id": "UniRef50_P47678", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47678", "UniRef:UniRef50_P47678" ] }, "pscc": { "uniref50_id": "UniRef50_P47678", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47678" ], "product": "Uncharacterized protein MG440" } }, "id": "KMADJIBOEG_993", "locus": "GCA000292445_00993" }, { "type": "cds", "sequence": "contig_1", "start": 545397, "stop": 545591, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGGTATTAGGTTCTTTAATTACAGCATGCTCTAACTTAAGCAGTGGAACAAATTTTTCCAGTTTAAACCAACTTAGAAATAGTTTCAGTAAAAATAGTGAATTAAATCAAGACAAGAAAGAGTTAGTTACTTCACTAAGAGATAGTTTTGAAGTGGATTCAAAAAGTGCTACAAACGTTCTATTAGATGCTTGA", "aa": "MVLGSLITACSNLSSGTNFSSLNQLRNSFSKNSELNQDKKELVTSLRDSFEVDSKSATNVLLDA", "aa_hexdigest": "11ffeae36c3bc70ac7bf65898d4e2301", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 39, "aa_cov": 0.609375, "hmm_cov": 1.0, "evalue": 1.0175489872274325e-10, "score": 36.658443450927734, "start": 26, "stop": 64 } ], "seq_stats": { "molecular_weight": 6926.618800000002, "isoelectric_point": 5.042550849914551 }, "id": "KMADJIBOEG_994", "locus": "GCA000292445_00994" }, { "type": "cds", "sequence": "contig_1", "start": 546314, "stop": 547126, "strand": "-", "frame": 3, "gene": "ylqF", "product": "ribosome biogenesis GTPase YlqF", "db_xrefs": [ "COG:COG1161", "COG:J", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0042254", "SO:0001217", "UniRef:UniRef50_P75135", "UniRef:UniRef90_Q49435" ], "nt": "ATGGACACATATACCTCCGCCAAAATTAATTGGTTCCCGGGTCACATGAAAAAGACCCATGATCAATTAAAAAAGTTATCTAGTCAAATTGATGGCATCATTGAGATAGTAGATGCTAGAGCACCAACTTTAACTCATAATTCAGAAATAATTAGTTATTTTTTAAATAAACCAAAATTAATACTGGCATTAAAGACAGATTTAGCACAATACAAACCAAATAAAAAGATCTTATTTGGTTCATTAAAAGAACCTTTTAAACTCAAAAAAAAAGTTCTAAAGACATTAACAACTTTATTTGCAAATAAAAGACAACAATTAAAAGCAAAAGGCTTATTAATCAAACAATTTAGATTAGCAGTTATTGGCATGCCCAATGTAGGTAAATCTAGTTTAATTAACCTTTTGATAAATAAAAATCACTTAAAAGTAGCTAACCGCGCTGGTATTACTAAATCATTAAATTGGATTCAAATTAGCCCTGAATTATTATTAAGTGATACCCCAGGAGTTTTTTTGAAAAGAATTGATGAAATTCAAATTGGATATAAACTAGTTTTGACTAATGTTATTAGAAGAGAAGTAGTTAATATAGAAGAAGTGGGAATGTTTGCTTTTAATTATCTTAAGAAACATTACAAACAGCTTTTACCATTTGAAGCGGATAGCTTTATTAACTTTTTGGAAAAATTTGCTAAAGTGAGAGGCTTAATTAAGAAAGCTAATGAACTAAACACTAACTTAGCATGTGAAATTTTTATTAATGAACTTATTAATGGGAAATATGGCAAATTAAGTTATGAACTTAATTAG", "aa": "MDTYTSAKINWFPGHMKKTHDQLKKLSSQIDGIIEIVDARAPTLTHNSEIISYFLNKPKLILALKTDLAQYKPNKKILFGSLKEPFKLKKKVLKTLTTLFANKRQQLKAKGLLIKQFRLAVIGMPNVGKSSLINLLINKNHLKVANRAGITKSLNWIQISPELLLSDTPGVFLKRIDEIQIGYKLVLTNVIRREVVNIEEVGMFAFNYLKKHYKQLLPFEADSFINFLEKFAKVRGLIKKANELNTNLACEIFINELINGKYGKLSYELN", "aa_hexdigest": "c8c7aab1de8e18407ee556cde78caee3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q49435", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.996, "score": 521.0, "evalue": 1.03e-186, "valid": true, "gene": "ylqF", "product": "ribosome biogenesis GTPase YlqF", "uniref50_id": "UniRef50_P75135", "cog_id": "COG1161", "cog_category": "J", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0042254" ] }, "pscc": { "uniref50_id": "UniRef50_P75135", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75135" ], "product": "Probable ribosome biogenesis GTPase A" }, "genes": [ "ylqF" ], "id": "KMADJIBOEG_995", "locus": "GCA000292445_00995" }, { "type": "cds", "sequence": "contig_1", "start": 547098, "stop": 547490, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10035.14" ], "nt": "TTGCAAGATGTCAATAATTATCGTGATTCTGCTTGGGAAGTTAGTTTATTTTTCTCACCTAATTTAATTGCAACTTTTTTTTCAATTTTGTTAACAGGAACAGTAGTTAGTTATCTTTTCCCTCGTTATAATTTTGCTGAAATTAAAGTATTTACTGATAAGCTTGAAGAAGTTAGAAAAGCATTGTTAAGTGATAATGCTAATCACAGTTTATCTATTCAAGAAACGCTTGGTGGTTATTCTTTACTCAAAAAGAAGATGATAGTTTCTGTTTCCATGTATGTAGAAATTCCCCATTTAATTAAGATTATTAGACAAATTGACAAGGATTGTTTGGTATCTATTACAAGAATCAGGGGAATAGATGGACACATATACCTCCGCCAAAATTAA", "aa": "MQDVNNYRDSAWEVSLFFSPNLIATFFSILLTGTVVSYLFPRYNFAEIKVFTDKLEEVRKALLSDNANHSLSIQETLGGYSLLKKKMIVSVSMYVEIPHLIKIIRQIDKDCLVSITRIRGIDGHIYLRQN", "aa_hexdigest": "20399d3d976eaedb0230aa5010c7455a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10035.14", "name": "DUF2179", "length": 47, "aa_cov": 0.36153846153846153, "hmm_cov": 1.0, "evalue": 5.328465184369164e-10, "score": 34.19164276123047, "start": 77, "stop": 123 } ], "seq_stats": { "molecular_weight": 14988.261899999989, "isoelectric_point": 8.7098237991333 }, "id": "KMADJIBOEG_996", "locus": "GCA000292445_00996" }, { "type": "cds", "sequence": "contig_1", "start": 547788, "stop": 548285, "strand": "-", "frame": 2, "gene": "yitT", "product": "putative protein MG443", "db_xrefs": [ "COG:COG1284", "COG:S", "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47681", "UniRef:UniRef90_P47681" ], "nt": "ATGAAATTTTTTAACAACTTATTTAAAAAAGAATCAAAGATAACAGTCGCATCTGGTTCTAAACGCGTAAGGATTTCTAACTCTTTTTTGATGTTTAGCAATCTTTATGAGGCTAAAAAACCATTAAAGTATGCATTAGTTTACTTACTATCAATTATTAATGCTTTTCTTTTACTTATTTTTATTCAAAAAACAGGTTTATATAGCTTTGGAATTAGTTCTTTAACTCAAGGTTTTGCTAGATTAGTGTTTGTTTTGTTAAAAAGTTTTGATGAAACTCAAAGATTATTAATTTTTAACATCCTTTACTGGCTTTTATATGTCTTTATCAACATCCCTTTAATTATTTTTTCTTATAAAAAAATAGGTAAAAACTTTACTATTCTTTCAACCCATTTTGTTGTTGCATCTAACGTATTTGGTTTTTTAATCAGTATTATTCCAGGTTCTGATAATTTACCACCAATGTTAGCTTCTATAACAGATACTAACTTTTGA", "aa": "MKFFNNLFKKESKITVASGSKRVRISNSFLMFSNLYEAKKPLKYALVYLLSIINAFLLLIFIQKTGLYSFGISSLTQGFARLVFVLLKSFDETQRLLIFNILYWLLYVFINIPLIIFSYKKIGKNFTILSTHFVVASNVFGFLISIIPGSDNLPPMLASITDTNF", "aa_hexdigest": "0b353873c024057913d6bb3ceb2610ef", "start_type": "ATG", "rbs_motif": null, "genes": [ "yitT" ], "truncated": "5-prime", "pseudogene": { "start": 547485, "stop": 548285, "observations": { "mutation": [ 547739, 547790 ], "stop": [ 547739, 547790 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47681", "query_cov": 1.0, "subject_cov": 0.4177215189873418, "identity": 0.9940000000000001, "score": 307.0, "evalue": 3.03e-102, "gene_start": 1, "gene_end": 165, "reference_start": 1, "reference_end": 165, "reference_sequence": "MKFFNNLFKKESKITVASGSKRVRISNSFLMFSNLYEAKKPLKYVLVYLLSIINAFLLLIFIQKTGLYSFGISSLTQGFARLVFVLLKSFDETQRLLIFNILYWLLYVFINIPLIIFSYKKIGKNFTILSTHFVVASNVFGFLISIIPGSDNLPPMLASITDTNFWKAAKDLNQSAGFVPFLWSDTSQGNVIISTFIYAAIYGFYNGISVSLLYILGGSAGGADFLTQYYARKKNRSVGSILFYVNSFILIIAILIGSFVAGSLLLQDVNNYRDSAWEVSLFFSPNLIATFFSILLTGTVVSYLFPRYNFAEIKVFTDKLEEVRKALLSDNANHSLSIQETLGGYSLLKKKMIVSVSMYVEIPHLIKIIRQIDKDCLVSITRIRGIDGHIYLRQN" }, "paralog": false, "identity": 0.9849624060150376, "score": 501.0, "evalue": 4.16e-181, "description": "Internal stop codon at 547739, 547790. Nonsense mutation around 547739, 547790.", "psc": { "uniref90_id": "UniRef90_P47681", "gene": "YitT", "product": "Uncharacterized protein MG443", "uniref50_id": "UniRef50_P47681", "cog_id": "COG1284", "cog_category": "S", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47681", "UniRef:UniRef50_P47681", "COG:COG1284", "COG:S", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47681", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47681" ], "product": "Uncharacterized protein MG443" } }, "id": "KMADJIBOEG_997", "locus": "GCA000292445_00997" }, { "type": "cds", "sequence": "contig_1", "start": 548301, "stop": 548660, "strand": "-", "frame": 2, "gene": "rplS", "product": "50S ribosomal protein L19", "db_xrefs": [ "COG:COG0335", "COG:J", "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02884", "RefSeq:WP_010869484.1", "SO:0001217", "UniParc:UPI0000133CB2", "UniRef:UniRef100_P47682", "UniRef:UniRef50_P47682", "UniRef:UniRef90_P47682" ], "nt": "ATGAAAAAAATAAATAAACAAGCATTAATTGATGCAGTAGAACAAAAACAGTTAAAGGAATATGTTCCTGAATTTGGAGCAGGAGATGAAGTTAATGTTGCTATTAAGTTACGTGAAAAAGAAAAAGTTCGAGTTCAAAACTTCACTGGAACTGTTTTAAGAAGAAGGGGAAGAGGGATTAGCGAAACTTTCATGGTAAGAAAAACCACTGATGGAATTCCTATTGAAAAAAACTTTCAAATCCACAACCCTAATATAGACATAGAAGTAAAACGCAGGGGTAAAGTAAGAAGAGCATACATCTCTTATATGCGTGAAAGATCTGGTAAGTCAGCAAAAATTAAAGAAAAAAAGTCTTAA", "aa": "MKKINKQALIDAVEQKQLKEYVPEFGAGDEVNVAIKLREKEKVRVQNFTGTVLRRRGRGISETFMVRKTTDGIPIEKNFQIHNPNIDIEVKRRGKVRRAYISYMRERSGKSAKIKEKKS", "aa_hexdigest": "69ed898e384468424d405a0c31417fa3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133CB2", "ncbi_nrp_id": "WP_010869484.1", "uniref100_id": "UniRef100_P47682", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133CB2", "RefSeq:WP_010869484.1", "UniRef:UniRef100_P47682" ] }, "ips": { "uniref100_id": "UniRef100_P47682", "uniref90_id": "UniRef90_P47682" }, "psc": { "uniref90_id": "UniRef90_P47682", "gene": "rplS", "product": "50S ribosomal protein L19", "uniref50_id": "UniRef50_P47682", "cog_id": "COG0335", "cog_category": "J", "kegg_orthology_id": "K02884", "go_ids": [ "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47682", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47682" ], "product": "Large ribosomal subunit protein bL19" }, "genes": [ "rplS" ], "id": "KMADJIBOEG_998", "locus": "GCA000292445_00998" }, { "type": "cds", "sequence": "contig_1", "start": 548644, "stop": 549186, "strand": "-", "frame": 1, "gene": null, "product": "tRNA (guanine-N(1)-)-methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B1GZ20" ], "nt": "ATGGCTTATGGTGGAGGAAGTGGCATGGTTTTAAAAGCTGAACCTATTATTAATTGTTTAAATTTTTATAAAGCCCCAAATTCTCATGTAGTTTTACTCTCCCCAGAAGGTGAACAATTTTCTCAGAATTGTGCTAAAAAACTTACAAAATACGAACACTTAATTTTGTTATCTGGTCACTATGAAGGTTTTGATCAAAGAATTTATAAATATATTGATCAAATTGTTTCTTTAGGTGATTTTGTTTTAAGTGGTGGGGAACTTGTAGCACTAAGTGTTATTGATGCTACTGTTAGATTAATTAAAGGAGTTATTAATGATCAGAGTCTTATTTGTGAATCATTCAATGATAATCTATTAGATTTTCCTGTTTATACAAGGCCATACGATTTAAAAGGCGATAAAGTTCCTGAAGTTTTACTTTCAGGAGATCACCAAAAGATTGAATCATTTCGTAAAGAACAGCAAATCTTAAAAACTGCAAAATACAGACCTGATTTATACAAAAAATACTTAGAAAATAAAAATGAAAAAAATAAATAA", "aa": "MAYGGGSGMVLKAEPIINCLNFYKAPNSHVVLLSPEGEQFSQNCAKKLTKYEHLILLSGHYEGFDQRIYKYIDQIVSLGDFVLSGGELVALSVIDATVRLIKGVINDQSLICESFNDNLLDFPVYTRPYDLKGDKVPEVLLSGDHQKIESFRKEQQILKTAKYRPDLYKKYLENKNEKNK", "aa_hexdigest": "d4452856f851b30e01abd183ade9b5d0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A534NV99", "query_cov": 0.9444444444444444, "subject_cov": 0.821256038647343, "identity": 0.512, "score": 167.0, "evalue": 3.19e-49, "valid": false, "gene": "trmD", "product": "tRNA (guanosine(37)-N1)-methyltransferase TrmD", "ec_ids": [ "2.1.1.228" ], "uniref50_id": "UniRef50_B1GZ20", "kegg_orthology_id": "K00554", "go_ids": [ "GO:0008033", "GO:0052906" ] }, "pscc": { "uniref50_id": "UniRef50_B1GZ20", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B1GZ20" ], "product": "tRNA (guanine-N(1)-)-methyltransferase" }, "genes": [], "id": "KMADJIBOEG_999", "locus": "GCA000292445_00999" }, { "type": "cds", "sequence": "contig_1", "start": 549226, "stop": 549339, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAATCACTGTTTTAACACTTTTTGAAAACACTATTTGGCCTTACTTAAATAGTTCTATTATGTTACAAGCTCAAAAAGCAAATTTAGTTCAATTTGAAGTAGTAAATTGA", "aa": "MKITVLTLFENTIWPYLNSSIMLQAQKANLVQFEVVN", "aa_hexdigest": "1cc73688319b6ba21c945286dd1b2383", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4298.030700000001, "isoelectric_point": 5.897352409362793 }, "id": "KMADJIBOEG_1000", "locus": "GCA000292445_01000" }, { "type": "cds", "sequence": "contig_1", "start": 549453, "stop": 549608, "strand": "-", "frame": 2, "gene": "rpsP", "product": "30S ribosomal protein S16", "db_xrefs": [ "COG:COG0228", "COG:J", "GO:0000314", "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0022627", "SO:0001217", "UniRef:UniRef50_P47684", "UniRef:UniRef90_P47684" ], "nt": "ATGCGGATGGGAAGAGTACACTATCCGCTTTATAGAATAGTAGCGGTTGATTCGCGAGTAAAGCGTAATGGAAAGTATATCGCTTTAATTGGACATCTAAATCCAGCTTTAAAGGAAAATAAGTGTAAATTAGATGAAACTGTTGCCTTAGATTGA", "aa": "MRMGRVHYPLYRIVAVDSRVKRNGKYIALIGHLNPALKENKCKLDETVALD", "aa_hexdigest": "b576cd2be2db58e53cd9ebf649b721c7", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpsP" ], "truncated": "5-prime", "pseudogene": { "start": 549339, "stop": 549626, "observations": { "mutation": [ 549455 ], "stop": [ 549455 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47684", "query_cov": 1.0, "subject_cov": 0.5368421052631579, "identity": 1.0, "score": 106.0, "evalue": 7.08e-29, "gene_start": 1, "gene_end": 51, "reference_start": 7, "reference_end": 57, "reference_sequence": "MVKIRLMRMGRVHYPLYRIVAVDSRVKRNGKYIALIGHLNPALKENKCKLDETVALDWLNKGAIPTDTVRSLFSESGLWKKFIESKNKKETSPKK" }, "paralog": false, "identity": 0.9789473684210527, "score": 187.0, "evalue": 1.85e-63, "description": "Internal stop codon at 549455. Nonsense mutation around 549455.", "psc": { "uniref90_id": "UniRef90_P47684", "gene": "rpsP", "product": "30S ribosomal protein S16", "uniref50_id": "UniRef50_P47684", "cog_id": "COG0228", "cog_category": "J", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0022627" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47684", "UniRef:UniRef50_P47684", "COG:COG0228", "COG:J", "GO:0000314", "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P47684", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47684" ], "product": "Small ribosomal subunit protein bS16" } }, "id": "KMADJIBOEG_1001", "locus": "GCA000292445_01001" }, { "type": "cds", "sequence": "contig_1", "start": 550235, "stop": 550372, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAACTGCAAATGCTGCCTTAGCAGGTTTAATTAATATTGCTTTTGGTCAAATTCACACTAATCTGTTTATGGATAAATATTTCGCTAATCCACCACAAAATAAGTTGGTCAAATTTATTGAAAAGTACTCATAA", "aa": "MLTANAALAGLINIAFGQIHTNLFMDKYFANPPQNKLVKFIEKYS", "aa_hexdigest": "9c40812b28e08467067f1c353f298c22", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5057.8851, "isoelectric_point": 9.30383548736572 }, "id": "KMADJIBOEG_1002", "locus": "GCA000292445_01002" }, { "type": "cds", "sequence": "contig_1", "start": 550472, "stop": 551263, "strand": "-", "frame": 3, "gene": null, "product": "MATE family efflux transporter", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FCE1E4D", "UniRef:UniRef90_UPI001ED8F93A" ], "nt": "TTGATTGGTCTTAACATCATTCCTATGCTTTCAAGGTTGTTTTTTTATTTAGCACAATCAGAGGGAAGACAACTTTTTATTGCAATTGTTCCGCCTATTGCTAATTTGATTAACATATTAATAGTTTTTTTATTAGTTAGATATTCTTCTTTAGGAGTAATAGGATCAGCTGTGGCTGGAATACTAGGATACTTAATTAATTTTTTAGCTTATATTATTTATCTAATTTATTTAAACAAAAGAAATCTTACTTATTTAACTTTTAAAACTATTAAATTAAACAAAATTGACTTTAATCTACTAGTTGTAGTTTCATTAATAGGAATGGCATCCTTTTTTAGAAATGGATCACTTTCTATTGTTACTACATTTTATGAGAGTTTTTTGGTTAATTTGACAAAAGCAACTACTGATAAAAATGATGTTTTTTATCTGACACTTTTAACTGGACCTATTGCTATTAGTAATTTAGCTTCAGCAGCTATTTTTGGTTTATTACAAGGAGTTAGAACAGTTTCATCATATAAGTTTGGACAAAAAAAATATGATGAAATTAAAAAAATTAACATTTATACAGTTATTATCTGTATATCGTTCGGTAGTTTAATCTATTTGTTAACAGCAGTTGCTTTTGGAAAACAGATCTTATCTAGTTTTTTTGATGTTTCAGATCAAAATTTGGATTTAGCAAATTACTTTTCATTAATAGTGCAAGCACAAGTTTTCTTTGTAGCCACAGGCGCTAATAGTCAACAATATTTTCAAAATACTAACAGAGTGCTTTATTCTTGA", "aa": "MIGLNIIPMLSRLFFYLAQSEGRQLFIAIVPPIANLINILIVFLLVRYSSLGVIGSAVAGILGYLINFLAYIIYLIYLNKRNLTYLTFKTIKLNKIDFNLLVVVSLIGMASFFRNGSLSIVTTFYESFLVNLTKATTDKNDVFYLTLLTGPIAISNLASAAIFGLLQGVRTVSSYKFGQKKYDEIKKINIYTVIICISFGSLIYLLTAVAFGKQILSSFFDVSDQNLDLANYFSLIVQAQVFFVATGANSQQYFQNTNRVLYS", "aa_hexdigest": "f9d69ed4664fb82e01aabcacc03f077a", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 550235, "stop": 551263, "observations": { "mutation": [ 550375, 550474 ], "stop": [ 550375, 550474 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001ED8F93A", "query_cov": 1.0, "subject_cov": 0.7690058479532164, "identity": 1.0, "score": 414.0, "evalue": 3.2e-143, "gene_start": 1, "gene_end": 263, "reference_start": 1, "reference_end": 263, "reference_sequence": "MIGLNIIPMLSRLFFYLAQSEGRQLFIAIVPPIANLINILIVFLLVRYSSLGVIGSAVAGILGYLINFLAYIIYLIYLNKRNLTYLTFKTIKLNKIDFNLLVVVSLIGMASFFRNGSLSIVTTFYESFLVNLTKATTDKNDVFYLTLLTGPIAISNLASAAIFGLLQGVRTVSSYKFGQKKYDEIKKINIYTVIICISFGSLIYLLTAVAFGKQILSSFFDVSDQNLDLANYFSLIVQAQVFFVATGANSQQYFQNTNRVLYSWIVSLTHGLFVFIPLLFIFQAITLQTNNIEVFIWLLTANAALAGLINIAFGQIHTNLFMDKYFANPPQNKLVKFIEKYS" }, "paralog": false, "identity": 0.9912280701754386, "score": 572.0, "evalue": 3.81e-208, "description": "Internal stop codon at 550375, 550474. Nonsense mutation around 550375, 550474.", "psc": { "uniref90_id": "UniRef90_UPI001ED8F93A", "product": "MATE family efflux transporter", "uniref50_id": "UniRef50_UPI002FCE1E4D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001ED8F93A", "UniRef:UniRef50_UPI002FCE1E4D" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FCE1E4D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FCE1E4D" ], "product": "MATE family efflux transporter" } }, "id": "KMADJIBOEG_1003", "locus": "GCA000292445_01003" }, { "type": "cds", "sequence": "contig_1", "start": 551450, "stop": 551878, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAACAGTAAAAGGGATCGTTTTGAAAAACAACTCCTAATTAAAAATGTTTTTGAATCAAAACAGCTTTTTCTAACTATTCTTAGATTTACTGTTCCAACTTTTTTCTTTGCACTTTTTAGTGCTGCTTATGTATTTATAGATCAAATCATGGTGATTAAGTTTGTTCCTAGAAGTGAACTTAATCCTGATTCTATTTTTACTGATCAGAGTTTAATTGATGAATTTAAAAATTCTGCTTTTTATAAAAGTGATAGTTTTTTAAGCTCTGGACTTAATATTAAACAATTTATTAAAACTGTTTTAAATATTAGTCAACCTTTGATTGTTTTGTTAAACGCCATTAATATTTTTATTCCTCTAGGAACTGGAGTTATTTTTTCTAAAGCAATTGGTAGAAATGATCAAAATAAAATTCAGGAAGCTTGA", "aa": "MNSKRDRFEKQLLIKNVFESKQLFLTILRFTVPTFFFALFSAAYVFIDQIMVIKFVPRSELNPDSIFTDQSLIDEFKNSAFYKSDSFLSSGLNIKQFIKTVLNISQPLIVLLNAINIFIPLGTGVIFSKAIGRNDQNKIQEA", "aa_hexdigest": "6abbd272c12a14bf8db4d667cd274ee9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16280.81949999998, "isoelectric_point": 9.474934577941895 }, "id": "KMADJIBOEG_1004", "locus": "GCA000292445_01004" }, { "type": "cds", "sequence": "contig_1", "start": 551879, "stop": 552064, "strand": "-", "frame": 3, "gene": null, "product": "peptide-methionine (R)-S-oxide reductase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAU5ZP34" ], "nt": "ATGATAAGAACTGAAGTTCGTGCTAAAAACAGTGATAGCCACTTGGGACATGTTTTTAATGATGGACCGAGTGAATTAGGTGGATTGCGCTATTGTATTAATTCTGCAGCTTTAAAATTTATTCCTTTTGAAGAGATGGAAAAATTAGGATACAAGGATTACATCCATCTTTTTAAAAATAAATAA", "aa": "MIRTEVRAKNSDSHLGHVFNDGPSELGGLRYCINSAALKFIPFEEMEKLGYKDYIHLFKNK", "aa_hexdigest": "cb3735859ffda3bb46315f0f2a15d0ca", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAU5ZP34", "query_cov": 0.9508196721311475, "subject_cov": 0.8656716417910447, "identity": 0.638, "score": 86.3, "evalue": 4.27e-21, "valid": false, "product": "peptide-methionine (R)-S-oxide reductase", "uniref50_id": "UniRef50_A0AAU5ZP34" }, "pscc": { "uniref50_id": "UniRef50_A0AAU5ZP34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAU5ZP34" ], "product": "peptide-methionine (R)-S-oxide reductase" }, "genes": [], "id": "KMADJIBOEG_1005", "locus": "GCA000292445_01005" }, { "type": "cds", "sequence": "contig_1", "start": 552128, "stop": 552331, "strand": "-", "frame": 3, "gene": "msrB", "product": "Peptide methionine sulfoxide reductase MsrB", "db_xrefs": [ "COG:COG0229", "COG:O", "EC:1.8.4.12", "GO:0006979", "GO:0030091", "GO:0033743", "SO:0001217", "UniRef:UniRef50_Q8E026", "UniRef:UniRef90_P47686" ], "nt": "ATGAGTAAATACCAAAAAAAGAGTGAAGCTGAGCTAAAACGTACTTTAACCAAACTCCAGTTTGATGTAACGCAAAATGCTCACACTGAGCCACCTTACATTAATGAATATAACCGCAATTTTGAAAAGGGAATTTATGTTGATATAACATCTGGTGAACCACTTTTTATTTCAACTGATAAATTTAAATCTGGTTGTGGTTGA", "aa": "MSKYQKKSEAELKRTLTKLQFDVTQNAHTEPPYINEYNRNFEKGIYVDITSGEPLFISTDKFKSGCG", "aa_hexdigest": "0f07e3d21a7b38b6e7204dc83e7933ea", "start_type": "ATG", "rbs_motif": null, "genes": [ "msrB" ], "truncated": "5-prime", "pseudogene": { "start": 551879, "stop": 552331, "observations": { "mutation": [ 552130 ], "stop": [ 552130 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47686", "query_cov": 1.0, "subject_cov": 0.44666666666666666, "identity": 1.0, "score": 139.0, "evalue": 4.02e-41, "gene_start": 1, "gene_end": 67, "reference_start": 1, "reference_end": 67, "reference_sequence": "MSKYQKKSEAELKRTLTKLQFDVTQNAHTEPPYINEYNRNFEKGIYVDITSGEPLFISTDKFKSGCGWPAFSKPIDKNLIANYRDESHGMIRTEVRAKNSDSHLGHVFNDGPSELGGLRYCINSAALKFIPFEEMEKLGYKDYIHLFKNK" }, "paralog": false, "identity": 0.9933333333333333, "score": 308.0, "evalue": 5.51e-110, "description": "Internal stop codon at 552130. Nonsense mutation around 552130.", "psc": { "uniref90_id": "UniRef90_P47686", "gene": "msrB", "product": "Peptide methionine sulfoxide reductase MsrB", "ec_ids": [ "1.8.4.12" ], "uniref50_id": "UniRef50_Q8E026", "cog_id": "COG0229", "cog_category": "O", "go_ids": [ "GO:0033743", "GO:0030091", "GO:0006979" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47686", "EC:1.8.4.12", "UniRef:UniRef50_Q8E026", "COG:COG0229", "COG:O", "GO:0033743", "GO:0030091", "GO:0006979" ] }, "pscc": { "uniref50_id": "UniRef50_Q8E026", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8E026" ], "product": "Peptide methionine sulfoxide reductase MsrB" } }, "id": "KMADJIBOEG_1006", "locus": "GCA000292445_01006" }, { "type": "cds", "sequence": "contig_1", "start": 552328, "stop": 552729, "strand": "-", "frame": 1, "gene": "ytpR", "product": "YtpR family tRNA-binding protein", "db_xrefs": [ "GO:0000049", "SO:0001217", "UniRef:UniRef50_P47687", "UniRef:UniRef90_P47687" ], "nt": "ATGAAAAGAATAAGTGAGCTTTTAGGTTATGATCTTATTTCATTAGCAAATAAAGTTCCTTTTGTTGTTTGTGAAGTTGTTTCAGTTATTCCTATTGCAAACACCCATCTAAAAAGGTGTAAAGTTAACACTGGTTTGACAAAAAGCTTGGATATTGTTTGTGGAGCTAATAATGTTAGAGTTGGTATGAAAACAGTTTTAGTACAAGTAGGTGGAGTGTTACCTAGCGGTACAGTTATCAAAAAAACTAAAATAGCTGGTTTTGATTCAGTTGGCATGTTATGTTCTGAAAAAGAACTTAATTTAAAACAGAAAAATGAGGGTATAATTGAGATTAATCCTAAGATCAAAGTAGGAAAACCATTTATTGATGTATATTTAAATAAACAACATTCCAAATGA", "aa": "MKRISELLGYDLISLANKVPFVVCEVVSVIPIANTHLKRCKVNTGLTKSLDIVCGANNVRVGMKTVLVQVGGVLPSGTVIKKTKIAGFDSVGMLCSEKELNLKQKNEGIIEINPKIKVGKPFIDVYLNKQHSK", "aa_hexdigest": "d4902a162519a9d81a112745d4fbe60f", "start_type": "ATG", "rbs_motif": null, "genes": [ "ytpR" ], "truncated": "both", "pseudogene": { "start": 552295, "stop": 552980, "observations": { "deletion": [ 552779 ], "mutation": [ 552857 ], "stop": [ 552329, 552857 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47687", "query_cov": 1.0, "subject_cov": 0.5833333333333334, "identity": 1.0, "score": 258.0, "evalue": 1.44e-85, "gene_start": 1, "gene_end": 133, "reference_start": 85, "reference_end": 217, "reference_sequence": "MFDISKDFVSIFYRKETLKNCMFGIIGSRSETTLRQQNDKDWSFFVNDANRITGFNFFNIKKSFKKFFLSHRFNEGLNYPSLKIMKRISELLGYDLISLANKVPFVVCEVVSVIPIANTHLKRCKVNTGLTKSLDIVCGANNVRVGMKTVLVQVGGVLPSGTVIKKTKIAGFDSVGMLCSEKELNLKQKNEGIIEINPKIKVGKPFIDVYLNKQHSKWVNTKKRVKLS" }, "paralog": false, "identity": 0.9912280701754386, "score": 440.0, "evalue": 4.64e-160, "description": "Internal stop codon at 552329, 552857. Frameshift due to deletion around 552779. Nonsense mutation around 552857.", "psc": { "uniref90_id": "UniRef90_P47687", "gene": "ytpR", "product": "YtpR family tRNA-binding protein", "uniref50_id": "UniRef50_P47687", "go_ids": [ "GO:0000049" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47687", "UniRef:UniRef50_P47687", "GO:0000049" ] }, "pscc": { "uniref50_id": "UniRef50_P47687", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47687" ], "product": "Uncharacterized protein MG449" } }, "id": "KMADJIBOEG_1007", "locus": "GCA000292445_01007" }, { "type": "cds", "sequence": "contig_1", "start": 552855, "stop": 552980, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGATATATCTAAAGATTTTGTTTCTATCTTTTATCGTAAAGAAACATTAAAAAACTGTATGTTTGGCATCATAGGAAGTAGAAGTGAAACAACTTTACGCCAACAAAATGACAAAGACTGA", "aa": "MFDISKDFVSIFYRKETLKNCMFGIIGSRSETTLRQQNDKD", "aa_hexdigest": "0beeec6f9cc5fde636670048409328d5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4863.506200000002, "isoelectric_point": 7.900616645812989 }, "id": "KMADJIBOEG_1008", "locus": "GCA000292445_01008" }, { "type": "cds", "sequence": "contig_1", "start": 552990, "stop": 553259, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAGTTTTTTAACAAAGCACAATCTGCTAGCAGTGCTCATAAGTTAGCAGTTCAATTTTTAGAAAAAAAGCTATTAAAAAAAGAAGTTAATTTCAGAAGAGCGGCTTTGTTGACAACAATGTTTGAAACTGATAAAAATCAACTCATAAAGCAAGAATTTGTTACGCTTCTCAACAATGCAGTTGATGTAAGTGAGCACTTGCTTTCACCTGTAATTTGTACACACACAGGTATCAATAGTTATGCCTTTTTAATACAAACAGAATAA", "aa": "MQFFNKAQSASSAHKLAVQFLEKKLLKKEVNFRRAALLTTMFETDKNQLIKQEFVTLLNNAVDVSEHLLSPVICTHTGINSYAFLIQTE", "aa_hexdigest": "30e4fe475650da5e6f639ddfeeb58777", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10142.645699999992, "isoelectric_point": 8.853910636901855 }, "id": "KMADJIBOEG_1009", "locus": "GCA000292445_01009" }, { "type": "cds", "sequence": "contig_1", "start": 553554, "stop": 553862, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGGATCGCTTTTTTAGTTGATTCTGTCAGTAATCTAAAGGAAGATAAAAATAGTCATCTCTATGTTTTATCGCTTTATATTATTGAAACAATTGCTGAACACCAAGAAACATTCAAATCAGGTTTTAATATTGATCTTAAAACCTTAACTGATAAGATGATTAACGCCCCTAAAGGTGTTAAGTTTTCAACATCGCAAACTTCTGAAGAAGAGGTTCGTGACAAAGTTAAGAGCATAATTAATGATTATGATCTTATCATAGGAATCCCTATTGATAAAGAGATCTCAACCTCCTATTTAAATTGA", "aa": "MRIAFLVDSVSNLKEDKNSHLYVLSLYIIETIAEHQETFKSGFNIDLKTLTDKMINAPKGVKFSTSQTSEEEVRDKVKSIINDYDLIIGIPIDKEISTSYLN", "aa_hexdigest": "8abaa85f83670ec0e02791a4b8d68771", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11613.10229999999, "isoelectric_point": 5.078984642028807 }, "id": "KMADJIBOEG_1010", "locus": "GCA000292445_01010" }, { "type": "cds", "sequence": "contig_1", "start": 553865, "stop": 555049, "strand": "-", "frame": 3, "gene": "tuf", "product": "elongation factor Tu", "db_xrefs": [ "COG:COG0050", "COG:J", "GO:0001968", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006414", "GO:0009897", "GO:0016020", "RefSeq:WP_009885583.1", "SO:0001217", "UniParc:UPI0000129DB5", "UniRef:UniRef100_P13927", "UniRef:UniRef50_P23568", "UniRef:UniRef90_P23568" ], "nt": "ATGGCAAGAGAGAAATTTGACCGTTCCAAACCACATGTCAATGTTGGTACCATTGGTCACATTGACCATGGTAAAACCACTTTAACAGCTGCTATCTGTACAGTTTTAGCAAAGGAAGGAAAATCAGCTGCAACGCGTTATGATGAAATTGATAAAGCCCCTGAAGAAAAAGCAAGGGGAATTACAATTAACTCTGCACACGTAGAATATTCTTCTGACAAACGTCACTATGCCCATGTTGACTGTCCTGGACATGCTGACTACATTAAAAACATGATCACAGGTGCTGCACAAATGGATGGAGCTATTCTAGTTGTTTCAGCAACTGATAGTGTGATGCCCCAAACCCGCGAGCACATCTTACTTGCCCGCCAAGTAGGGGTTCCTAAAATGGTAGTTTTTCTAAACAAGTGTGATATTGCTAGTGATGAAGAGGTACAAGAACTTGTTGCTGAAGAAGTACGTGATCTGTTAACTTCCTATGGTTTTGATGGTAAGAACACTCCTATTATTTATGGCTCAGCTTTAAAAGCATTGGAAGGTGATCCAAAGTGGGAGGCTAAGATCCATGATTTGATTAAAGCAGTTGATGAATGGATTCCAACTCCTACACGTGAAGTAGATAAACCTTTCTTATTAGCAATTGAAGATACGATGACCATTACTGGTAGAGGTACAGTTGTTACAGGAAGAGTTGAAAGAGGTGAACTCAAAGTAGGTCAAGAAGTTGAAATTGTTGGTTTAAAACCAATTAGAAAAGCAGTTGTTACTGGAATTGAAATGTTCAAAAAGGAACTTGATTCAGCAATGGCTGGTGACAATGCTGGGGTATTATTACGTGGTGTTGAACGTAAAGAAGTTGAAAGAGGTCAAGTTTTAGCAAAACCAGGCTCTATTAAACCGCACAAGAAATTTAAAGCTGAGATCTATGCTTTAAAGAAAGAAGAAGGTGGCAGACACACTGGTTTTTTAAACGGTTACCGTCCTCAATTCTATTTCCGTACCACTGATGTAACTGGTTCTATTGCTTTAGCTGAAAATACTGAAATGGTTCTACCTGGTGATAATGCTTCTATTACTGTTGAGTTAATTGCTCCTATTGCTTGTGAAAAAGGTAGTAAGTTCTCAATTCGTGAAGGTGGCAGAACTGTAGGGGCAGGCACTGTAACAGAAGTTCTAGAATAA", "aa": "MAREKFDRSKPHVNVGTIGHIDHGKTTLTAAICTVLAKEGKSAATRYDEIDKAPEEKARGITINSAHVEYSSDKRHYAHVDCPGHADYIKNMITGAAQMDGAILVVSATDSVMPQTREHILLARQVGVPKMVVFLNKCDIASDEEVQELVAEEVRDLLTSYGFDGKNTPIIYGSALKALEGDPKWEAKIHDLIKAVDEWIPTPTREVDKPFLLAIEDTMTITGRGTVVTGRVERGELKVGQEVEIVGLKPIRKAVVTGIEMFKKELDSAMAGDNAGVLLRGVERKEVERGQVLAKPGSIKPHKKFKAEIYALKKEEGGRHTGFLNGYRPQFYFRTTDVTGSIALAENTEMVLPGDNASITVELIAPIACEKGSKFSIREGGRTVGAGTVTEVLE", "aa_hexdigest": "61ef27a09c235dba715d0dea52d18464", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000129DB5", "ncbi_nrp_id": "WP_009885583.1", "uniref100_id": "UniRef100_P13927", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000129DB5", "RefSeq:WP_009885583.1", "UniRef:UniRef100_P13927" ] }, "ips": { "uniref100_id": "UniRef100_P13927", "uniref90_id": "UniRef90_P23568" }, "psc": { "uniref90_id": "UniRef90_P23568", "gene": "tuf", "product": "elongation factor Tu", "uniref50_id": "UniRef50_P23568", "cog_id": "COG0050", "cog_category": "J", "go_ids": [ "GO:0001968", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006414", "GO:0009897", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P23568", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P23568" ], "product": "Elongation factor Tu" }, "genes": [ "tuf" ], "id": "KMADJIBOEG_1011", "locus": "GCA000292445_01011" }, { "type": "cds", "sequence": "contig_1", "start": 555219, "stop": 555368, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTCACTAGTGCTTTTGTTTTAACTGGAATTGTTGAAAGCCTTTTAACATTTGGAACTATTATTGAACAAATTGATAAATTCACTGATCAGACTAATGTGATGTTATTAATTTATGCAGTTATCTACACTTTTAATCCAAAAAGTTGA", "aa": "MVTSAFVLTGIVESLLTFGTIIEQIDKFTDQTNVMLLIYAVIYTFNPKS", "aa_hexdigest": "78fe6aba90e9a10c824b534ff9edf733", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5487.3813, "isoelectric_point": 4.316091728210449 }, "id": "KMADJIBOEG_1012", "locus": "GCA000292445_01012" }, { "type": "cds", "sequence": "contig_1", "start": 555369, "stop": 555746, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MG452", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75125", "UniRef:UniRef90_P47690" ], "nt": "TTGTTAAAAAACCAACAATTCTTTTTAAGTGCATTAGCTTATATATTATTTACTTTTATTGGCTATAACCTAATTTTGTCAATAGCTGGTATAGCTTATAAATCAACAAATCCATATAAGTTAACAAGTAGTATTTTTCTCCATGTAATTGCACCAATAGCATTCTTCATAGCAAGTTTTATCAAAATAAAACATGAGAAAGATGTCAATATTAACATGTTCTTTAAAAGCCTATTATTATTCATGATCTATCCTTTAATATATGGGCTTTATTTAGTAACTATTCCATATGTAAGGCATTATCTTTTTAATGGTAGGCCATCTACTTATACAATTTATGGCAGCATTACAAATACTAAAGATAATCCTTTTGCTTGA", "aa": "MLKNQQFFLSALAYILFTFIGYNLILSIAGIAYKSTNPYKLTSSIFLHVIAPIAFFIASFIKIKHEKDVNINMFFKSLLLFMIYPLIYGLYLVTIPYVRHYLFNGRPSTYTIYGSITNTKDNPFA", "aa_hexdigest": "ae7f3cd4bacbeb2d438900d641a82cd6", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 555102, "stop": 555887, "observations": { "mutation": [ 555162, 555165, 555366, 555744, 555873 ], "stop": [ 555162, 555165, 555366, 555744, 555873 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47690", "query_cov": 1.0, "subject_cov": 0.4789272030651341, "identity": 0.9840000000000001, "score": 238.0, "evalue": 1.74e-77, "gene_start": 1, "gene_end": 125, "reference_start": 90, "reference_end": 214, "reference_sequence": "MLIIFKDLNLVKTNNCQIIGWWKQLSLPQKTFLSFIPLFLVTSAFVLTGIVESLLTFGTIIEQIDKFTDQTNVMLLIYAVIYTFNPKSWLLKNQQFFLSALAYILFTFIGYNLILSIAGIAYKSTNPYKLTSSIFLHVIAPIAFFIASFIKIKHEKDVNINMFFKSLLLFMIYPLIYGLYLVTIPYVRHYLFNGRPSTYTIYGSITNTKNNPFAWLVVFAVLFIYFPLSYLAIYLLQLKLIKKAIQPQFNLPFTLNKWKQK" }, "paralog": false, "identity": 0.9731800766283525, "score": 490.0, "evalue": 1.8e-179, "description": "Internal stop codon at 555162, 555165, 555366, 555744, 555873. Nonsense mutation around 555162, 555165, 555366, 555744, 555873.", "psc": { "uniref90_id": "UniRef90_P47690", "product": "Uncharacterized protein MG452", "uniref50_id": "UniRef50_P75125", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47690", "UniRef:UniRef50_P75125", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75125", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75125" ], "product": "Uncharacterized protein MG452 homolog" } }, "id": "KMADJIBOEG_1013", "locus": "GCA000292445_01013" }, { "type": "cds", "sequence": "contig_1", "start": 555872, "stop": 556750, "strand": "+", "frame": 2, "gene": "galU", "product": "UTP--glucose-1-phosphate uridylyltransferase GalU", "db_xrefs": [ "COG:COG1210", "COG:M", "EC:2.7.7.9", "GO:0003983", "GO:0006011", "GO:0009058", "RefSeq:WP_009885581.1", "SO:0001217", "UniParc:UPI0000053FBB", "UniRef:UniRef100_P47691", "UniRef:UniRef50_P47691", "UniRef:UniRef90_P47691" ], "nt": "ATGAAAACAAAAATAAGAAAAGCAGTTATTCCTGCTGCTGGGTTGGGTGTTAGGTTACTACCAGCAACAAAAGCAATTCCCAAAGAGATGTTACCATTGGTAAATAAACCTACTATCCAATACATAGTAGAGGAAGCAGTTAAAAGTGGCATTGAACAGATTCTTGTCATTGTTTCATCCAAAAAAACAGCTATATTAGATCATTTTGATTATGATCTGATCTTAGAAAATGCCTTAATTCAAAAAAATAAATTGCAGGAGCATAAAGAGATTGAAGATATTGCTAATTTAGCACATATCTTTTTTGTTAGACAAAAAAATCAAGATGGTTTGGGAGATGCAATCTTGTTTGCTGAATCTTTTGTTGGTAATGAAGACTTTGCAGTATTGTTAGGTGATGATGTTGTTTTTAGTAAAGAACCTGCTTTAAAACAATGCTTGGAAGCTTATTATGAAACTAATTGTCAAACAATCGGTGTACAAGAAGTAGATCCTTGTCATGTTGATAAGTATGGAATTATCACCCCTGAAGGTGATTACAAAAATAAAGATCTTATTAAGGTTTTAGCAATGACTGAAAAACCTAAACCAAAAGATGCTAAAAGTAATTTAGCAATCTTAGGGCGATATGTACTCAAACCATCTATTTTCAAAGCACTTAGAAGTGTACCTTATGGAGTTGGTGGTGAGTTGCAGCTAACTGATGGTTTAAATTTTTGTTTGAAAAATGAAAACTTTTATGCAAGAAAGTTTACTGGTACTAGGTTTGATGTTGGCACAAAGAGTGGTTTTATTAAAGCAAATTTATTTACTGCTTTAAACAATAAAGATATTAGTAAAAAAGAAGTTTTAGAACTTTTAAATTTAGTTAAAGCTTAA", "aa": "MKTKIRKAVIPAAGLGVRLLPATKAIPKEMLPLVNKPTIQYIVEEAVKSGIEQILVIVSSKKTAILDHFDYDLILENALIQKNKLQEHKEIEDIANLAHIFFVRQKNQDGLGDAILFAESFVGNEDFAVLLGDDVVFSKEPALKQCLEAYYETNCQTIGVQEVDPCHVDKYGIITPEGDYKNKDLIKVLAMTEKPKPKDAKSNLAILGRYVLKPSIFKALRSVPYGVGGELQLTDGLNFCLKNENFYARKFTGTRFDVGTKSGFIKANLFTALNNKDISKKEVLELLNLVKA", "aa_hexdigest": "1107b311c79eb86ee11611e83b4f1807", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000053FBB", "ncbi_nrp_id": "WP_009885581.1", "uniref100_id": "UniRef100_P47691", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000053FBB", "RefSeq:WP_009885581.1", "UniRef:UniRef100_P47691" ] }, "ips": { "uniref100_id": "UniRef100_P47691", "uniref90_id": "UniRef90_P47691" }, "psc": { "uniref90_id": "UniRef90_P47691", "gene": "galU", "product": "UTP--glucose-1-phosphate uridylyltransferase GalU", "ec_ids": [ "2.7.7.9" ], "uniref50_id": "UniRef50_P47691", "cog_id": "COG1210", "cog_category": "M", "go_ids": [ "GO:0003983", "GO:0006011", "GO:0009058" ] }, "pscc": { "uniref50_id": "UniRef50_P47691", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47691" ], "product": "UTP--glucose-1-phosphate uridylyltransferase" }, "genes": [ "galU" ], "id": "KMADJIBOEG_1014", "locus": "GCA000292445_01014" }, { "type": "cds", "sequence": "contig_1", "start": 556740, "stop": 557162, "strand": "-", "frame": 2, "gene": "osmC", "product": "Organic hydroperoxide resistance protein-like", "db_xrefs": [ "COG:COG1764", "COG:V", "EC:1.11.1.28", "GO:0006979", "KEGG:K04063", "RefSeq:WP_014894083.1", "SO:0001217", "UniParc:UPI00027B317B", "UniRef:UniRef100_UPI00027B317B", "UniRef:UniRef50_P47692", "UniRef:UniRef90_P47692" ], "nt": "ATGGCATTGATTTATAAAACTGTTGCACAAACTGAAACTGGGAGAGAAGGTAGTGTTAAAACTTTAGATGGTTTTCAAACAAAACTTAGTTTTCCCAAACCTGATTTATCAGTTCAAACAGAAAATAATCCTGAGCAGTTATTTGCATCTGCTTATGCAAGTTGTTTTTCTCAAGCAGTAATTGTTGTTATGCAACAACATCAATTTAGTTTTTCAAAAAAACCAGTTGTAAGTGTTAAAGTAGAACTCCATCAAGAAAATGGACTATTTCACATTAAAGCTGGTGTTGAATTAACTACTAATAGTAATGACCAAGAAGTTGGTAAAAAACTAATCAAAAAAGCCCATGAAATGTGTCCTTTTAGTCGATTGATTAGAAGTGAAAACTTCCTTGGTTTAACTTTAAATGGTATTAAGCTTTAA", "aa": "MALIYKTVAQTETGREGSVKTLDGFQTKLSFPKPDLSVQTENNPEQLFASAYASCFSQAVIVVMQQHQFSFSKKPVVSVKVELHQENGLFHIKAGVELTTNSNDQEVGKKLIKKAHEMCPFSRLIRSENFLGLTLNGIKL", "aa_hexdigest": "c5128bbefd9ab4c63f701eb7e3ba6eb5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B317B", "ncbi_nrp_id": "WP_014894083.1", "uniref100_id": "UniRef100_UPI00027B317B", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B317B", "RefSeq:WP_014894083.1", "UniRef:UniRef100_UPI00027B317B" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B317B", "uniref90_id": "UniRef90_P47692" }, "psc": { "uniref90_id": "UniRef90_P47692", "gene": "OsmC", "product": "Organic hydroperoxide resistance protein-like", "ec_ids": [ "1.11.1.28" ], "uniref50_id": "UniRef50_P47692", "cog_id": "COG1764", "cog_category": "V", "kegg_orthology_id": "K04063", "go_ids": [ "GO:0006979" ] }, "pscc": { "uniref50_id": "UniRef50_P47692", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47692" ], "product": "Organic hydroperoxide resistance protein-like" }, "genes": [ "osmC" ], "id": "KMADJIBOEG_1015", "locus": "GCA000292445_01015" }, { "type": "cds", "sequence": "contig_1", "start": 557195, "stop": 557560, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAATAACATATTGCAATTTCTCAAAGAAAGAGAACTTTATTCACAAGCTAATTTTGAAACAGAACTAGATAACCATTTAAAAGAGAAAAAAATTAACTTTTATGTTGGTTTTGATCCAACTGCTAATTCTTTACATATTGGCAATTATGTTTTAATTCACATTGCAAAATTATTAAAAGACATGGGGCATACTCCACACATAGTTCTAGGGAGTGCAACTGCTTTAATTGGTGATCCTACTGGCAGAATTGAATTAAGGAAAATTTTAGAAGAAAAAGAAATTGTAAAAAACACCAAAACAATTAAACAACAAATCAAACAGTTTTTAGGTGATGTAATTATTCATGAAAACAAAGTTTGA", "aa": "MLNNILQFLKERELYSQANFETELDNHLKEKKINFYVGFDPTANSLHIGNYVLIHIAKLLKDMGHTPHIVLGSATALIGDPTGRIELRKILEEKEIVKNTKTIKQQIKQFLGDVIIHENKV", "aa_hexdigest": "7506d3fd3d80ad3a4ca0b1711229815c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13888.984899999983, "isoelectric_point": 7.988938331604004 }, "id": "KMADJIBOEG_1016", "locus": "GCA000292445_01016" }, { "type": "cds", "sequence": "contig_1", "start": 557675, "stop": 557833, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATTAAACTATATGATCTTACAAGCATATGACTTTTATTATCTACATAAAAACCATAATGTCACTTTACAAATAGGTGGAAGTGATCAGTGGGCTAATATTTTGGCTGGTGCTAACTTAATTAAAAGAAAAATAATGCTAATGTTTTTGGATTAA", "aa": "MELNYMILQAYDFYYLHKNHNVTLQIGGSDQWANILAGANLIKRKIMLMFLD", "aa_hexdigest": "d73fb9f5e2207d8a536e810aa5f780cb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6134.134099999999, "isoelectric_point": 6.701737785339356 }, "id": "KMADJIBOEG_1017", "locus": "GCA000292445_01017" }, { "type": "cds", "sequence": "contig_1", "start": 557983, "stop": 558315, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01479.30", "PFAM:PF22421.1" ], "nt": "ATGCTAACAATGCTTGATAAAAAAGTAATAGATGAATTGTGTAATTTAAAAGGCCCAAAAATTAAACAAACCAAGCAAATGCTAGCCTTTTTAATTACTGAATTAGTCCATGGCACTAAAAAAGCAAAAGAAGCACAACAACGATCTGAACTAATATTTAGTAATCAACCAGATCTTGATATTAAGTTAGTAAAAACAAGCACTAATCTGATTGATTATTTAGTTGAAACTAAATTTATTAAAAGTAAATCAGAAGCAAGAAGATTAATTAGTCAAAAAGGTTTGACAATTAACAATAAACACGTTTTGGACTTAAACCAAATAATTGAATGA", "aa": "MLTMLDKKVIDELCNLKGPKIKQTKQMLAFLITELVHGTKKAKEAQQRSELIFSNQPDLDIKLVKTSTNLIDYLVETKFIKSKSEARRLISQKGLTINNKHVLDLNQIIE", "aa_hexdigest": "92a5cebc32e184035a6875b868cb11e9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01479.30", "name": "S4", "length": 39, "aa_cov": 0.35454545454545455, "hmm_cov": 1.0, "evalue": 1.385651040767252e-09, "score": 32.761024475097656, "start": 71, "stop": 109 }, { "id": "PF22421.1", "name": "SYY_C-terminal", "length": 57, "aa_cov": 0.5181818181818182, "hmm_cov": 0.9649122807017544, "evalue": 6.367716617720938e-11, "score": 37.22304153442383, "start": 53, "stop": 109 } ], "seq_stats": { "molecular_weight": 12620.776799999983, "isoelectric_point": 9.519611167907716 }, "id": "KMADJIBOEG_1018", "locus": "GCA000292445_01018" }, { "type": "cds", "sequence": "contig_1", "start": 558373, "stop": 559089, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MG456", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47694", "UniRef:UniRef90_P47694" ], "nt": "ATGGGTAATTTCAAGGCAATAGAATCATTTGAATTTTTTGAACAAGGTCCTATAAGCAGCGATATATTACCAAGTTTTTATCCAACTGCTCTTTACAATTTTGAACCACTTCCAAGACAATTTAAAGTTACTTATAAAGATGGTAGTGAATTTGTTTTTGGTCATATTTATGCAATTAAAAGAAGCAGTAATAAAACAGAAAAAGTAGCTTGCTTGATTGCTATTACACCAGCAATTAATAGTGAAAATCATTTCTTTTTAACTGATGCTAACTATTCTTTAAATAAGAATGTGGCTCAGTTTGAAGCACTTACTGAAAACAAAAAGCAAGAAAACATCTCACTTTTTGTTGAAAAAGATTCAAATTTTTCTTTTCAGAATTTAGATACAGAGGTATTAAATAAAGTGCTTTTTAATCCTTTGAATGTATATGCCGAATTTAATGTCTATAATGACACAGTTCATACTTACTTGTTTATGAGTGTGCCTACAACCTTTATGGATACTAAACTAAAAGTGAATGAAGCATTTGATGATCTAGTTACAAACATTAAACTCCAAGCAAGCTATGATTTCAAAACATTAAATTCAATGCAGAAAGTTGTTGATTTACTTCATAACAAATTAATCAAAAAACCAGAAAGTAAAAGCTCTAGTCAAAAAAGTGTTGAAACTGAAATAGAAAAAGAAGTTAAAGATAAATTAGCTAAGAATTAA", "aa": "MGNFKAIESFEFFEQGPISSDILPSFYPTALYNFEPLPRQFKVTYKDGSEFVFGHIYAIKRSSNKTEKVACLIAITPAINSENHFFLTDANYSLNKNVAQFEALTENKKQENISLFVEKDSNFSFQNLDTEVLNKVLFNPLNVYAEFNVYNDTVHTYLFMSVPTTFMDTKLKVNEAFDDLVTNIKLQASYDFKTLNSMQKVVDLLHNKLIKKPESKSSSQKSVETEIEKEVKDKLAKN", "aa_hexdigest": "39bf944ed9f9c3a84d3fb61fee5b0bc8", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 558373, "stop": 559377, "observations": { "mutation": [ 559101 ], "stop": [ 559101 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47694", "query_cov": 1.0, "subject_cov": 0.7125748502994012, "identity": 0.996, "score": 458.0, "evalue": 2.79e-161, "gene_start": 1, "gene_end": 238, "reference_start": 97, "reference_end": 334, "reference_sequence": "MSDLTKFNKFFLTPNKLNAFLRVIGLCGLFSVIAISFGIYSYTRNEIPNIASLFLIVLGSVVLFLAFVIHFAALFKRNKLLKKVNKENRTNLWQKEMGNFKAIESFEFFEQGPISSDILPSFYPTALYNFEPLPRQFKVTYKDGSEFVFGHIYAIKRSSNKTEKVACLIAITPAINSENHFFLTDANYSLNKNVAQFEALTENKKQENISLFVEKDSNFSFQNLDTEVLNKVLFNPLNVYAKFNVYNDTVHTYLFMSVPTTFMDTKLKVNEAFDDLVTNIKLQASYDFKTLNSMQKVVDLLHNKLIKKPESKSSSQKSVETEIEKEVKDKLAKN" }, "paralog": false, "identity": 0.9940119760479041, "score": 652.0, "evalue": 1.67e-240, "description": "Internal stop codon at 559101. Nonsense mutation around 559101.", "psc": { "uniref90_id": "UniRef90_P47694", "product": "Uncharacterized protein MG456", "uniref50_id": "UniRef50_P47694", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47694", "UniRef:UniRef50_P47694", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47694", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47694" ], "product": "Uncharacterized protein MG456" } }, "id": "KMADJIBOEG_1019", "locus": "GCA000292445_01019" }, { "type": "cds", "sequence": "contig_1", "start": 559099, "stop": 559377, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGATCTAACTAAATTTAACAAGTTCTTTTTGACACCAAATAAACTCAATGCTTTTCTAAGAGTAATTGGACTTTGTGGTCTTTTTAGTGTTATTGCTATCTCATTTGGTATTTATAGTTATACACGTAATGAAATACCAAATATAGCATCACTTTTTTTAATTGTTTTAGGTAGTGTTGTTTTGTTTTTAGCATTTGTTATCCATTTTGCTGCTCTTTTTAAAAGAAACAAGCTATTAAAAAAAGTTAACAAAGAAAACAGAACTAATTTATGA", "aa": "MSDLTKFNKFFLTPNKLNAFLRVIGLCGLFSVIAISFGIYSYTRNEIPNIASLFLIVLGSVVLFLAFVIHFAALFKRNKLLKKVNKENRTNL", "aa_hexdigest": "85b08d3165963612172bb14e8b2f55bd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10457.435899999984, "isoelectric_point": 10.358344841003415 }, "id": "KMADJIBOEG_1020", "locus": "GCA000292445_01020" }, { "type": "cds", "sequence": "contig_1", "start": 559725, "stop": 559946, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAACAAACAGTTAGTGTTGTTGATTTTATTGATAACTTCTTGGTTAACTTACTATCATTTAAGCAAGAATTAAATGCAATAGAAGTTGGTTATGGTAATGAAGCTTGGAATTGTTTTAAAGTACTAAATGATCAAAGTAAAACAATTACTGATTTTTTTACAGTAATTAAAAATAATCCCCAATTGTTTGACAGTGAATATCACTACATTTTGATTTGA", "aa": "MKQTVSVVDFIDNFLVNLLSFKQELNAIEVGYGNEAWNCFKVLNDQSKTITDFFTVIKNNPQLFDSEYHYILI", "aa_hexdigest": "d49fdaf039ab9cdc6a2134aa7bc293af", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8524.595899999998, "isoelectric_point": 4.5550428390502935 }, "id": "KMADJIBOEG_1021", "locus": "GCA000292445_01021" }, { "type": "cds", "sequence": "contig_1", "start": 559930, "stop": 561357, "strand": "-", "frame": 1, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V6LLW0" ], "nt": "GTGAAATTTACCAATATTGCTGGACTTCAAGAGGAAAAGCATGAGTTGCTTGAGATAGTTGATTATTTAAAAAATCCATTGAAATATGCCCAGATGGGAGCAAGATCCCCACGTGGGGTAATTTTATATGGTCCACCTGGGACAGGTAAAACATTATTAGCTAAAGCAGTAGCTGGTGAAGCTGGTGTTCCTTTCTTTCAATCAACGGGTTCTGGATTTGAAGATATGCTTGTTGGTGTTGGTGCTAAACGGGTTAGAGATCTTTTCAATAAAGCTAAAAAGGCTGCTCCTTGTATTATTTTTATTGATGAAATTGATTCAGTTGGTTCTAAACGGGGTAGAGTTGAACTCTCTTCTTATTCTGTTGTTGAGCAAACCTTAAACCAATTGTTAGCTGAAATGGATGGATTTACAAGCAGAACAGGTGTTGTTGTAATGGCAGCTACAAATAGGTTAGATGTATTAGATGATGCATTATTAAGACCTGGAAGATTTGATAGACATATTCAAATCAATCTCCCTGATATTAAAGAAAGGGAAGGGATTTTAAAAGTTCATGCTGAAAATAAAAATCTCTCTTCTAAGATAAGTCTTTTAGATGTTGCTAAGAGAACTCCTGGGTTTTCAGGTGCTCAATTAGAAAATGTTATCAATGAAGCTACATTGTTAGCAGTTAGAGACAACCGTACCACAATTAACATTAATGACATTGATGAAGCAATTGATAGAGTAATAGCTGGTCCTGCTAAAAAGTCACGTGTAATTAGTGATGAAGATAGAAAACTAGTTGCTTATCATGAGGCTGGTCATGCCTTGGTTGGTTTACATGTCCACAGTAATGATGAAGTACAAAAGATTACCATTATTCCTCGTGGTCAAGCAGGGGGTTACACACTTTCAACACCTAAGAGTGGTGATCTTAACCTAAAAAGAAAATCTGATTTACTTGCAATGATAGCAACTGCTATGGGCGGTAGAGCTGCTGAAGAGGAAATCTATGGTAATTTAGAAATTACTACTGGCGCTTCTAGCGATTTTTATAAAGCAACTAATATTGCAAGAGCAATGGTAACCCAGCTTGGGATGTCTAAATTAGGTCAAGTGCAATATGTACCAAGTCAAGGTACACTCCCTTCTAATGTAAAACTTTATTCAGAACAAACTGCTAAAGATATTGACAATGAGATTAATTTCATTATTGAAGAACAGTATAAGAAAGCAAAAACAATCATTAAGAGTAACCGTAAGGAACTAGAATTGCTTGTAGAAGCACTTTTAATTGCTGAAACTATTTTGAAAAGTGATATTGACTTCATCCATAAAAACACTAAACTACCACCAGAAATCTTATTGCAAAAGCAAGAACAACAAGCAAAGCAAAAACTAAATAAATCTGAAGTAAAACCAGAAAGTGAAACAAACAGTTAG", "aa": "MKFTNIAGLQEEKHELLEIVDYLKNPLKYAQMGARSPRGVILYGPPGTGKTLLAKAVAGEAGVPFFQSTGSGFEDMLVGVGAKRVRDLFNKAKKAAPCIIFIDEIDSVGSKRGRVELSSYSVVEQTLNQLLAEMDGFTSRTGVVVMAATNRLDVLDDALLRPGRFDRHIQINLPDIKEREGILKVHAENKNLSSKISLLDVAKRTPGFSGAQLENVINEATLLAVRDNRTTININDIDEAIDRVIAGPAKKSRVISDEDRKLVAYHEAGHALVGLHVHSNDEVQKITIIPRGQAGGYTLSTPKSGDLNLKRKSDLLAMIATAMGGRAAEEEIYGNLEITTGASSDFYKATNIARAMVTQLGMSKLGQVQYVPSQGTLPSNVKLYSEQTAKDIDNEINFIIEEQYKKAKTIIKSNRKELELLVEALLIAETILKSDIDFIHKNTKLPPEILLQKQEQQAKQKLNKSEVKPESETNS", "aa_hexdigest": "a4de579dd6ef6f0a905f228af8bba544", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A929X996", "query_cov": 0.9852631578947368, "subject_cov": 0.8283185840707965, "identity": 0.551, "score": 470.0, "evalue": 9.03e-159, "valid": false, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "uniref50_id": "UniRef50_A0A7V6LLW0", "go_ids": [ "GO:0004222", "GO:0006508", "GO:0016020", "GO:0016887", "GO:0051301" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7V6LLW0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V6LLW0" ], "product": "ATP-dependent zinc metalloprotease FtsH" }, "genes": [ "ftsH" ], "id": "KMADJIBOEG_1022", "locus": "GCA000292445_01022" }, { "type": "cds", "sequence": "contig_1", "start": 561448, "stop": 561606, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGAATGGTGGTTCTAGTAATAACACAAAGAGTATTGCAGGTTTTGAAACCCTTGGTACTTTCATTGCTCCTGATACTAGAGCTAGAGATGTATTAAATGGTTTGTTTGGCTTGCTACCAATTATTATCTTTGTAGTTTTCTTTTTACTCTTTTGA", "aa": "MMNGGSSNNTKSIAGFETLGTFIAPDTRARDVLNGLFGLLPIIIFVVFFLLF", "aa_hexdigest": "cd04ed211c784284b2dcf23ac4be6cc0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5637.570399999995, "isoelectric_point": 5.87689037322998 }, "id": "KMADJIBOEG_1023", "locus": "GCA000292445_01023" }, { "type": "cds", "sequence": "contig_1", "start": 562033, "stop": 562191, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTTGGTTCAAGGCTTTTATTGCACTTTTAAGTAAACATAAAAAGATAATTTTCGTCACTTTATTAACACTTATTAGTTTCATTACCATTGGCAGTATAATTACAGTTAATGTAGTTCAAACCAAAAAAACCCAAATCGGTCAAAAACTAACATAG", "aa": "MRWFKAFIALLSKHKKIIFVTLLTLISFITIGSIITVNVVQTKKTQIGQKLT", "aa_hexdigest": "4d3a29634f9a271594a38a2b92c049f1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5932.243300000001, "isoelectric_point": 11.471713829040528 }, "id": "KMADJIBOEG_1024", "locus": "GCA000292445_01024" }, { "type": "cds", "sequence": "contig_1", "start": 562210, "stop": 562737, "strand": "-", "frame": 1, "gene": "hpt", "product": "hypoxanthine phosphoribosyltransferase", "db_xrefs": [ "COG:COG0634", "COG:F", "EC:2.4.2.8", "GO:0000166", "GO:0000287", "GO:0004422", "GO:0005829", "GO:0006166", "GO:0006178", "GO:0032263", "GO:0032264", "GO:0046100", "GO:0052657", "KEGG:K00760", "RefSeq:WP_009885573.1", "SO:0001217", "UniParc:UPI000012CB64", "UniRef:UniRef100_P47696", "UniRef:UniRef50_P47696", "UniRef:UniRef90_P47696" ], "nt": "ATGGGTATTAAATCTATTGTTATTAATGAACAACAGATAGAAGAAGGCTGTCAAAAAGCAGTTAATTGGTGCAATGCTAAATTTAATAATAAAAAGGTAATTGTTCTTGGCATTCTAAAAGGTTGCATCCCTTTCCTTGGCAAAGTGATAAGTAAATTTAGTTTTGACCTCCAACTAGATTTTATGGCAGTTGCTTCTTATCATGGTTCACATGTACAAAAACAACCACCTAAGATTGTGCTTGATATGTCCCATGACCCTAAAGATAAAGACATCCTTTTAATAGAAGATATTGTTGATAGTGGTAGATCTATTAAATTAGTTATTGATCTTCTAAAAACAAGGCATGCTAAATCAATAACTTTAATTAGCTTAATTGAAAAGATTAAACCCAAAGCCTTTGATATTAATATTGATTTTTCTTGTTTTAAAGTAAAAGATAATTTTTTGGTTGGCTTTGGTCTTGACTATGATGGTTTTTATCGTAACCTACCTTATGTTGGTGTGTTTGAACCAGACAATCCCTAA", "aa": "MGIKSIVINEQQIEEGCQKAVNWCNAKFNNKKVIVLGILKGCIPFLGKVISKFSFDLQLDFMAVASYHGSHVQKQPPKIVLDMSHDPKDKDILLIEDIVDSGRSIKLVIDLLKTRHAKSITLISLIEKIKPKAFDINIDFSCFKVKDNFLVGFGLDYDGFYRNLPYVGVFEPDNP", "aa_hexdigest": "4242e5c1e66cb0cecb4953ec06aac97f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012CB64", "ncbi_nrp_id": "WP_009885573.1", "uniref100_id": "UniRef100_P47696", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012CB64", "RefSeq:WP_009885573.1", "UniRef:UniRef100_P47696" ] }, "ips": { "uniref100_id": "UniRef100_P47696", "uniref90_id": "UniRef90_P47696" }, "psc": { "uniref90_id": "UniRef90_P47696", "gene": "hpt", "product": "hypoxanthine phosphoribosyltransferase", "ec_ids": [ "2.4.2.8" ], "uniref50_id": "UniRef50_P47696", "cog_id": "COG0634", "cog_category": "F", "kegg_orthology_id": "K00760", "go_ids": [ "GO:0000166", "GO:0000287", "GO:0004422", "GO:0005829", "GO:0006166", "GO:0006178", "GO:0032263", "GO:0032264", "GO:0046100", "GO:0052657" ] }, "pscc": { "uniref50_id": "UniRef50_P47696", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47696" ], "product": "Hypoxanthine-guanine phosphoribosyltransferase" }, "genes": [ "hpt" ], "id": "KMADJIBOEG_1025", "locus": "GCA000292445_01025" }, { "type": "cds", "sequence": "contig_1", "start": 562940, "stop": 563248, "strand": "-", "frame": 3, "gene": "ispF", "product": "putative protein MG459", "db_xrefs": [ "COG:COG0245", "COG:I", "GO:0008685", "GO:0016114", "SO:0001217", "UniRef:UniRef50_Q49436", "UniRef:UniRef90_Q49436" ], "nt": "ATGCAGTTAAGAGTTGGCCTAGGAAGCAAAAAATACCACATAAAATTAAGGAAAGAAAGTAAAAATAAATTTTGGTTAGGCGGTGTTGATTTTGAGAATTCTAAATATATTTATGATCTTGAAAACAGTGATGAAGTTAGTGATGTTATCCAATTAGCAGTTGCTGATGCTTTATTTGGTGCAACTGCATTAGGTGATGGTCATATTGTTTTCAATAAAGAAAAATCAACAGTCCAATTTAAAGGTACTGCAAAAAAAGAAGCACCAAGAGTCTTAGCAAGAACTTACAATTTCATCAGAAAAAACTGA", "aa": "MQLRVGLGSKKYHIKLRKESKNKFWLGGVDFENSKYIYDLENSDEVSDVIQLAVADALFGATALGDGHIVFNKEKSTVQFKGTAKKEAPRVLARTYNFIRKN", "aa_hexdigest": "56d3e83cfeccc1fa6a186aecde3bae45", "start_type": "ATG", "rbs_motif": "TTATA", "genes": [ "ispF" ], "truncated": "5-prime", "pseudogene": { "start": 562739, "stop": 563248, "observations": { "mutation": [ 562942 ], "stop": [ 562942 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49436", "query_cov": 1.0, "subject_cov": 0.6035502958579881, "identity": 0.99, "score": 199.0, "evalue": 1.14e-63, "gene_start": 1, "gene_end": 102, "reference_start": 1, "reference_end": 102, "reference_sequence": "MQLRVGLGSKKYHIKLRKESKNKFWLGGVDFENSKYIYDLENSDEVSDVIQLAVADALFGATALGDGHIVFNKGKSTVQFKGTAKKEAPRVLARTYNFIRKNWIINNIDISLEIPQEQKVDDYKHAIFAFICSALRISELTINLKVRKPLDSNEINCLAVVLVERQKTK" }, "paralog": false, "identity": 0.9822485207100592, "score": 326.0, "evalue": 1.96e-116, "description": "Internal stop codon at 562942. Nonsense mutation around 562942.", "psc": { "uniref90_id": "UniRef90_Q49436", "gene": "IspF", "product": "Uncharacterized protein MG459", "uniref50_id": "UniRef50_Q49436", "cog_id": "COG0245", "cog_category": "I", "go_ids": [ "GO:0008685", "GO:0016114" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49436", "UniRef:UniRef50_Q49436", "COG:COG0245", "COG:I", "GO:0008685", "GO:0016114" ] }, "pscc": { "uniref50_id": "UniRef50_Q49436", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49436" ], "product": "Uncharacterized protein MG459" } }, "id": "KMADJIBOEG_1026", "locus": "GCA000292445_01026" }, { "type": "cds", "sequence": "contig_1", "start": 563421, "stop": 564359, "strand": "+", "frame": 3, "gene": "ldh", "product": "L-lactate dehydrogenase", "db_xrefs": [ "COG:C", "COG:COG0039", "EC:1.1.1.27", "GO:0004459", "GO:0005737", "GO:0006089", "GO:0006090", "GO:0006096", "KEGG:K00016", "RefSeq:WP_009885571.1", "SO:0001217", "UniParc:UPI000012E33D", "UniRef:UniRef100_P47698", "UniRef:UniRef50_P78007", "UniRef:UniRef90_P47698" ], "nt": "ATGAAAGGACCTAAAATTGCAATAGTAGGATCAGGTGCTGTTGGTACTAGTTTTCTATATGCTGCAATGACTCGTGCACTTGGTAGTGAATACATGATTATAGATATCAATGAAAAAGCTAAAGTTGGTAATGTATTTGATCTTCAAGATGCTTCCTCATCATGCCCAAATTTTGGCAAAGTAGTTGCTGGAGAATACAGTCAATTAAAAGATTATGATTTTATTTTCATTAGTGCAGGAAGACCTCAAAAACAAGGTGGGGAAACTAGGTTGCAGCTACTTGAAGGCAATGTAGAAATTATGAAAAGCATTGCCAAAGAAATCAAAAAATCTGGTTTTAATGGAGTTACATTAATCGCTTCAAACCCAGTTGATATCATGTCATATACATATCTTAAAGTTACTGGATTTGAACCTAATAAAGTAATTGGCTCTGGTACTTTACTTGATAGTGCAAGATTAAGATATGCAATTGCAACTAAGTACCAAATGAGTTCAAAGGATGTACAAGCATATGTTATTGGTGAACATGGTGATAGTTCAGTAAGTATTATTTCATCAGCTAAAATTGCAGGACTATCACTAAAACATTTTTCTAAAGCTAGTGATATTGAAAAAGAATTTGGTGAAATTGACCAATTTATCAGACGCAGAGCTTATGAAATTATTGAACGCAAAGGTGCTACTTTCTATGGAATTGGTGAAGCTAGTGCTGATGTAGCTGAACAAATTTTGAAAGATACTAAAGAAGTTAGAGTAGTGGCTCCTTTACTTACTGGTCAGTATGGAGCGAAGGATATGATGTTTGGAACTCCTTGTGTACTTTCAAGAAAAGGTATTGAAAAGATCTTGGAAATAGAACTTTCAAATACTGAAAAAGTTGCGCTTGAAAATTCAATTAAAGTTTTGAAAGACAACATTAAACTAGCAAAGCTTTAG", "aa": "MKGPKIAIVGSGAVGTSFLYAAMTRALGSEYMIIDINEKAKVGNVFDLQDASSSCPNFGKVVAGEYSQLKDYDFIFISAGRPQKQGGETRLQLLEGNVEIMKSIAKEIKKSGFNGVTLIASNPVDIMSYTYLKVTGFEPNKVIGSGTLLDSARLRYAIATKYQMSSKDVQAYVIGEHGDSSVSIISSAKIAGLSLKHFSKASDIEKEFGEIDQFIRRRAYEIIERKGATFYGIGEASADVAEQILKDTKEVRVVAPLLTGQYGAKDMMFGTPCVLSRKGIEKILEIELSNTEKVALENSIKVLKDNIKLAKL", "aa_hexdigest": "99940a250ddf1c25b4687a3ef06d9c6e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012E33D", "ncbi_nrp_id": "WP_009885571.1", "uniref100_id": "UniRef100_P47698", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012E33D", "RefSeq:WP_009885571.1", "UniRef:UniRef100_P47698" ] }, "ips": { "uniref100_id": "UniRef100_P47698", "uniref90_id": "UniRef90_P47698" }, "psc": { "uniref90_id": "UniRef90_P47698", "gene": "ldh", "product": "L-lactate dehydrogenase", "ec_ids": [ "1.1.1.27" ], "uniref50_id": "UniRef50_P78007", "cog_id": "COG0039", "cog_category": "C", "kegg_orthology_id": "K00016", "go_ids": [ "GO:0004459", "GO:0005737", "GO:0006089", "GO:0006090", "GO:0006096" ] }, "pscc": { "uniref50_id": "UniRef50_P78007", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78007" ], "product": "L-lactate dehydrogenase" }, "genes": [ "ldh" ], "id": "KMADJIBOEG_1027", "locus": "GCA000292445_01027" }, { "type": "cds", "sequence": "contig_1", "start": 564503, "stop": 564772, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAAAAAAAGTTTATTAACTTGTTTTGTGAAGTTAGATGATTATGTTTTTCACTCATTTTTAGTTAATACATTTGAAACAACTAAAGATGCAATATTAAAAACCTTACTAGATAGTTATCTTAACACTCTGAAATTTCAAGTAAAGTTTTATGAAAATTGTGAAAAACGTGATCTTGATTTTGAATTAAAAGTGAAAGAATATCAAACTCCTAGTTATTTCATTACTAAATTCAATAACCAATTTAAGGGTTTTTATGAAGGATGA", "aa": "MFKKSLLTCFVKLDDYVFHSFLVNTFETTKDAILKTLLDSYLNTLKFQVKFYENCEKRDLDFELKVKEYQTPSYFITKFNNQFKGFYEG", "aa_hexdigest": "bd34176d64215970c3633baacc3661f3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10758.263399999993, "isoelectric_point": 7.705798530578614 }, "id": "KMADJIBOEG_1028", "locus": "GCA000292445_01028" }, { "type": "cds", "sequence": "contig_1", "start": 564875, "stop": 565633, "strand": "-", "frame": 3, "gene": "ydhJ", "product": "putative protein MG461", "db_xrefs": [ "COG:COG1078", "COG:R", "GO:0006203", "GO:0008832", "SO:0001217", "UniRef:UniRef50_P47699", "UniRef:UniRef90_P47699" ], "nt": "ATGCAACAAACCTTTTTTAAAGATCCTATTTTAGGAGAAATTATTTTTGATGAAAACACAAAGTGGATGTATGAATTGGTCAATACAAAAGCTTTTCAAAGGTTAAGAAACATCAAACAATTAGGAATTAACTTCCATTTTTATCCTAGTGGAGTTCACACTAGGTACGCCCATTCTTTAGGAGTTTATGAACTGATAAGAAGGATCTTAAATAGTTCTGCATTTTTAAATATTGATCAAATTAAAAAACAAACTGTTTTAGTAGCTGGTTTGTTGCATGATCTTGGTCATGGACCTCATTCTCATGCTTTTGAAATCTATTTTGCTAAAAATCCTGATTTTAAAAAACAACTTTTTATTCATGAAAAGGTTACTTCAATGTTAGTTAATAGTGAACCTATTGTCTCTATCTTAAAAGCTAATAAGATAGATCCAAATCTGATTGGCGCTTTAATTGATGAAAATCAAAATATTCAACCTATTAACTGGTGGATGAGACAGCTAATATCTTCAGATCTTGACACAGACAGAATGGATTATTTATTACGTGATGCTTATTTTACAGGTACCAGTCATTCATTAGTTGATTATCAATCAATTATCAATGGAATGGAATGTGTAGATAATCAGGGCACTTATGAAATTGTTTTTCAAGAAAAATGCTTACCTTTCATTGAAAACTTCTTGATAACAAGACACCACATGTATCAATCAATCTACAGTGATGGTAGAAGTATTGCTACAGAACTTAACTTATGA", "aa": "MQQTFFKDPILGEIIFDENTKWMYELVNTKAFQRLRNIKQLGINFHFYPSGVHTRYAHSLGVYELIRRILNSSAFLNIDQIKKQTVLVAGLLHDLGHGPHSHAFEIYFAKNPDFKKQLFIHEKVTSMLVNSEPIVSILKANKIDPNLIGALIDENQNIQPINWWMRQLISSDLDTDRMDYLLRDAYFTGTSHSLVDYQSIINGMECVDNQGTYEIVFQEKCLPFIENFLITRHHMYQSIYSDGRSIATELNL", "aa_hexdigest": "41720e1b1bc383fcce95409d307405d8", "start_type": "ATG", "rbs_motif": null, "genes": [ "ydhJ" ], "truncated": "5-prime", "pseudogene": { "start": 564572, "stop": 565633, "observations": { "mutation": [ 564877 ], "stop": [ 564877 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47699", "query_cov": 1.0, "subject_cov": 0.5929411764705882, "identity": 1.0, "score": 515.0, "evalue": 2.59e-182, "gene_start": 1, "gene_end": 252, "reference_start": 1, "reference_end": 252, "reference_sequence": "MQQTFFKDPILGEIIFDENTKWMYELVNTKAFQRLRNIKQLGINFHFYPSGVHTRYAHSLGVYELIRRILNSSAFLNIDQIKKQTVLVAGLLHDLGHGPHSHAFEIYFAKNPDFKKQLFIHEKVTSMLVNSEPIVSILKANKIDPNLIGALIDENQNIQPINWWMRQLISSDLDTDRMDYLLRDAYFTGTSHSLVDYQSIINGMECVDNQGTYEIVFQEKCLPFIENFLITRHHMYQSIYSDGRSIATELNLWFVFQRIKALIEEDNFNFHNFKNVESVIKPLLKNQLFKKSLLTCFVKLDDYVFHSFLVNTFETTKDAILKTLLDSYLNTLKFQVKFYESCEKRDLDFELKVKEYQTPSYFITKFNNQFKGFYEGWNKHKNELKIKTSQNKIKNLSEISMLVKRSNELFFENSFYRWANVFYQN" }, "paralog": false, "identity": 0.9943342776203966, "score": 711.0, "evalue": 6.18e-262, "description": "Internal stop codon at 564877. Nonsense mutation around 564877.", "psc": { "uniref90_id": "UniRef90_P47699", "gene": "YdhJ", "product": "Uncharacterized protein MG461", "uniref50_id": "UniRef50_P47699", "cog_id": "COG1078", "cog_category": "R", "go_ids": [ "GO:0008832", "GO:0006203" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47699", "UniRef:UniRef50_P47699", "COG:COG1078", "COG:R", "GO:0008832", "GO:0006203" ] }, "pscc": { "uniref50_id": "UniRef50_P47699", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47699" ], "product": "Uncharacterized protein MG461" } }, "id": "KMADJIBOEG_1029", "locus": "GCA000292445_01029" }, { "type": "cds", "sequence": "contig_1", "start": 565614, "stop": 565772, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF19269.4" ], "nt": "GTGTTTAACTTAAAAGGTAAACAACTTTTTATGCCAATAAGGTTAATTTTTACAAATAAGGAGCATGGACCTGATTTAGCACATATTATTGAAATTTTTGATAAAGAAAGTGCAATAAACTTAATTAAGCAATTTATTAATGCAACAAACCTTTTTTAA", "aa": "MFNLKGKQLFMPIRLIFTNKEHGPDLAHIIEIFDKESAINLIKQFINATNLF", "aa_hexdigest": "df98cf08eec54401ee998c3ec3e4ea52", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF19269.4", "name": "Anticodon_2", "length": 43, "aa_cov": 0.8269230769230769, "hmm_cov": 1.0, "evalue": 2.7383399226940534e-08, "score": 29.451568603515625, "start": 3, "stop": 45 } ], "seq_stats": { "molecular_weight": 6074.122299999998, "isoelectric_point": 8.181634330749514 }, "id": "KMADJIBOEG_1030", "locus": "GCA000292445_01030" }, { "type": "cds", "sequence": "contig_1", "start": 565815, "stop": 566036, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATGTTAAGGTTGATTATTTAAAAGATAAAAACAGGGAAATAAGTTTACTTTTTAAAAATCAAATAACCCATGGTGTTCAAATAAACGAATTGATAAGAGAATCTTTTGCCACTAAAATAGGTGTTGAAAACTTAGCTAAGAAAAGTCATATTTTGTTTAAAAACATCAAACTTTTTTTAGAACAGCTTGCCAAATCTTTACAAGGGTTGGAAGAATGA", "aa": "MDVKVDYLKDKNREISLLFKNQITHGVQINELIRESFATKIGVENLAKKSHILFKNIKLFLEQLAKSLQGLEE", "aa_hexdigest": "4d338d3cba2ef8fdbfe49036ece3fb04", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8469.808099999997, "isoelectric_point": 9.004702186584474 }, "id": "KMADJIBOEG_1031", "locus": "GCA000292445_01031" }, { "type": "cds", "sequence": "contig_1", "start": 566211, "stop": 566453, "strand": "-", "frame": 2, "gene": null, "product": "glutamate--tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003594285F" ], "nt": "ATGGAAATTACTGATGTTTTAAGGGGAGCTGAGCACATCTCTAACACTGCATACCAACTTGCTATATATCAAGCATTAGGTTTTAAAAGAATTCCCCGCTTTGGTCATCTTTCAGTTATTGTTGATGAAAGTGGCAAAAAACTTTCTAAACGTGATGAGAAAACTACTCAGTTTATTGAGCAGTTTAAACAACAAGGCTATCTACCTGAAGCATTATTAAATTTCTTAGCACTCTTAGGTTGA", "aa": "MEITDVLRGAEHISNTAYQLAIYQALGFKRIPRFGHLSVIVDESGKKLSKRDEKTTQFIEQFKQQGYLPEALLNFLALLG", "aa_hexdigest": "648d89fc9c6321a5d78c0a33260bc56c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003594285F", "query_cov": 0.875, "subject_cov": 0.8235294117647058, "identity": 0.6, "score": 85.9, "evalue": 2.12e-20, "valid": false, "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_UPI003594285F" }, "pscc": { "uniref50_id": "UniRef50_UPI003594285F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003594285F" ], "product": "glutamate--tRNA ligase family protein" }, "genes": [], "id": "KMADJIBOEG_1032", "locus": "GCA000292445_01032" }, { "type": "cds", "sequence": "contig_1", "start": 566571, "stop": 567068, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00749.27" ], "nt": "ATGGAAAAAATTAGAACACGTTATGCACCATCCCCAACAGGATATCTGCATGTTGGTGGTACAAGAACAGCAATCTTTAACTTTTTACTAGCCAAGCACTTTAATGGTGAGTTTATTATCAGGATAGAAGATACTGATACTGAAAGAAACATAAAAGAAGGAATTAATTCACAATTTGATAACTTGCGTTGGCTTGGAGTCATTGCAGATGAATCGGTTTATAACCCTGGCAATTATGGTCCATATCTGCAATCACAAAAACTAGCAGTTTATAAAAAACTAGCATTTGATTTAATTGAAAAAAATCTGGCATATCGTTGCTTCTGTAGCAAAGAAAAATTAGAGTCAGATAGAAAACAAGCCATTAATAACCACAAAACCCCTAAATACTTAGGTCATTGTCGTAATTTACATTCCAAGAAAATTACTAATCACTTAGAAAAAAATGATCCTTTTACTATCCGCTTAAAAATAAACAATGAAGCTGAATATAGTTGA", "aa": "MEKIRTRYAPSPTGYLHVGGTRTAIFNFLLAKHFNGEFIIRIEDTDTERNIKEGINSQFDNLRWLGVIADESVYNPGNYGPYLQSQKLAVYKKLAFDLIEKNLAYRCFCSKEKLESDRKQAINNHKTPKYLGHCRNLHSKKITNHLEKNDPFTIRLKINNEAEYS", "aa_hexdigest": "a1bd8d8224c3b4a54fc6b7dc7310f2bc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00749.27", "name": "tRNA-synt_1c", "length": 163, "aa_cov": 0.9757575757575757, "hmm_cov": 0.950920245398773, "evalue": 1.2969588628273166e-54, "score": 180.3483428955078, "start": 3, "stop": 163 } ], "seq_stats": { "molecular_weight": 19212.628600000015, "isoelectric_point": 9.282238578796388 }, "id": "KMADJIBOEG_1033", "locus": "GCA000292445_01033" }, { "type": "cds", "sequence": "contig_1", "start": 567055, "stop": 567834, "strand": "-", "frame": 1, "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "db_xrefs": [ "COG:COG0030", "COG:J", "EC:2.1.1.182", "GO:0000179", "GO:0003723", "GO:0005829", "GO:0006364", "GO:0031167", "GO:0052908", "RefSeq:WP_010869490.1", "SO:0001217", "UniParc:UPI000012E0AD", "UniRef:UniRef100_P47701", "UniRef:UniRef50_P47701", "UniRef:UniRef90_P47701" ], "nt": "GTGAATAGTTTTTTTCCTTCACGTAAATTAGGTCAAAATTTTACGGTTAATTTAAGCGTCATTAAAAGAATTTTTGCTTTTGTTAAAAATTTAAATCCACAAGCAATTGTTGAAATAGGTGTTGGTAAAGGAGCGTTAACAAATTATTTGTTAAAACTCAAAATACCTTACAAGGGGATAGAAATTGATAAACGCTTAATTGAATATCTTCTAGTTGAAAAGATATTAACTGAAGACCAACTAGTTAAAGGCGATATTCTCAAAAAGGACTTTAATAGTTTTTTTGAAAATTTAAGTCCATTATTGTGTGGTAATATCCCATATAGCATTACATCTCCAATAATCAATAAGTTTTTAGAATCAAAGCTTCGAAGCTTTGTTTTAATGACACAAAAAGAGTTTGCTAATCGGCTTCTGGCAAAGGTTAATTCTAGTGATTATAGTGCCTTTGGTGCTTTTTGTCAATACTATTTGACTATTACAACAGTTTTTAAAATTGATAGGCATGCTTTTAAACCTAAACCTAAGGTGGATTCAACCTTAATATTATTAGAAAAAAATAAATCGGTTAGTTATGACTTTAAGTTTGGTCTGTTTTTAAAGCAATGTTTCAATCAAAGAAGGAAGATGTTAATTAATAACTTAAAGCATTTTTTTGCAGTTGATTATTTACTTAACATTATTCAAAAGCAAAATTTAAAGACTAGCATTAGAGCGCAAGAGTTGAGTCCTTGTCAACTCTTTAATCTTTACCAAAACATTTGTAATGGAAAAAATTAG", "aa": "MNSFFPSRKLGQNFTVNLSVIKRIFAFVKNLNPQAIVEIGVGKGALTNYLLKLKIPYKGIEIDKRLIEYLLVEKILTEDQLVKGDILKKDFNSFFENLSPLLCGNIPYSITSPIINKFLESKLRSFVLMTQKEFANRLLAKVNSSDYSAFGAFCQYYLTITTVFKIDRHAFKPKPKVDSTLILLEKNKSVSYDFKFGLFLKQCFNQRRKMLINNLKHFFAVDYLLNIIQKQNLKTSIRAQELSPCQLFNLYQNICNGKN", "aa_hexdigest": "54b49b755d41550070f0724426524017", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012E0AD", "ncbi_nrp_id": "WP_010869490.1", "uniref100_id": "UniRef100_P47701", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012E0AD", "RefSeq:WP_010869490.1", "UniRef:UniRef100_P47701" ] }, "ips": { "uniref100_id": "UniRef100_P47701", "uniref90_id": "UniRef90_P47701" }, "psc": { "uniref90_id": "UniRef90_P47701", "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "ec_ids": [ "2.1.1.182" ], "uniref50_id": "UniRef50_P47701", "cog_id": "COG0030", "cog_category": "J", "go_ids": [ "GO:0000179", "GO:0003723", "GO:0005829", "GO:0006364", "GO:0031167", "GO:0052908" ] }, "pscc": { "uniref50_id": "UniRef50_P47701", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47701" ], "product": "Ribosomal RNA small subunit methyltransferase A" }, "genes": [ "rsmA" ], "id": "KMADJIBOEG_1034", "locus": "GCA000292445_01034" }, { "type": "cds", "sequence": "contig_1", "start": 567827, "stop": 568141, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTGTTTTACCAGTCCAATTTCTCTCTCAAAAACTTCCTCAAGTGTGGGCAAGTAAGCGTAATGAGAATGCGAAAGCCCATTCTCAAAAAAGTATTGAACAACTCAACAAAACTAAAAAGATGCAATTAATTTTTTACTTTGTGTTTGCTGCTATTACTGCATTTAGTGCTGCTGGAGTTGGTGTTTATTGGTTTTTAAATGCTTTGTTTACTTTATTACAATCTTATTTAACCCATGTTTTCATTGTCAAAAGAAGGGAAAAAAGAAAGCAAAATTATTCCAAATTAGATTTGATTTTAGAGCGTGAATAG", "aa": "MIVLPVQFLSQKLPQVWASKRNENAKAHSQKSIEQLNKTKKMQLIFYFVFAAITAFSAAGVGVYWFLNALFTLLQSYLTHVFIVKRREKRKQNYSKLDLILERE", "aa_hexdigest": "3b9395dc01ddeff6ab975b8eff9e27a7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12209.249899999986, "isoelectric_point": 10.167453956604003 }, "id": "KMADJIBOEG_1035", "locus": "GCA000292445_01035" }, { "type": "cds", "sequence": "contig_1", "start": 568160, "stop": 568588, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGCAACCAGAGTTCCACTAGGTAGCGGGGTGGAACTTGGGTTTAACATGATTCTTTCATTAATAGTACTTTTACTATTAGTTCGCTTGATAACTATTGTGATTACCTTAAACTCTACTTTAGCACTTGAAAAGATGAATGAAGTGCAAGGTAAATTAGCAGAAATTAACGCTAAATATAAAGGGGCAATAGATTTACAAAGTAAAAGAAACAGACAGTTAGAGATAATGTCACTTTATAAAAAGCACAACATTAAATCATCTGCTGCTTTTGTTCAAGTTTTTGTAACTTTACCTATCTTTTTAATTATTTATAGGATTGTTACTACTTTAAGACCAATTAAAGCAATCATCTTATTTAACTTTTGGGATCTTTCAAAAGTACCTTTAACAGAAATTTTCTCTAATTTCACTACAACTGGTTGA", "aa": "MYATRVPLGSGVELGFNMILSLIVLLLLVRLITIVITLNSTLALEKMNEVQGKLAEINAKYKGAIDLQSKRNRQLEIMSLYKKHNIKSSAAFVQVFVTLPIFLIIYRIVTTLRPIKAIILFNFWDLSKVPLTEIFSNFTTTG", "aa_hexdigest": "4e3e1113c7868ad5bacbfa31eb30f007", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16044.083099999989, "isoelectric_point": 9.992873573303225 }, "id": "KMADJIBOEG_1036", "locus": "GCA000292445_01036" }, { "type": "cds", "sequence": "contig_1", "start": 569311, "stop": 569457, "strand": "-", "frame": 1, "gene": "rpmH", "product": "50S ribosomal protein L34", "db_xrefs": [ "COG:COG0230", "COG:J", "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:1990904", "KEGG:K02914", "RefSeq:WP_009885567.1", "SO:0001217", "UniParc:UPI0000133FB9", "UniRef:UniRef100_P47704", "UniRef:UniRef50_P0A7P5", "UniRef:UniRef90_P47704" ], "nt": "ATGAAACGAACATACCAACCAAGCAAATTAAAGCGTGCTAAAACCCATGGTTTTATGGCTAGGATGGCAACTGCACAAGGACGTAAAGTTTTAAGGCAAAGACGTTTTAAAAATCGTGCTCAACTCACGGTTTCCAGTGAGCGTTAA", "aa": "MKRTYQPSKLKRAKTHGFMARMATAQGRKVLRQRRFKNRAQLTVSSER", "aa_hexdigest": "b7a51c6f33bc053523970850e910c399", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133FB9", "ncbi_nrp_id": "WP_009885567.1", "uniref100_id": "UniRef100_P47704", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133FB9", "RefSeq:WP_009885567.1", "UniRef:UniRef100_P47704" ] }, "ips": { "uniref100_id": "UniRef100_P47704", "uniref90_id": "UniRef90_P47704" }, "psc": { "uniref90_id": "UniRef90_P47704", "gene": "rpmH", "product": "50S ribosomal protein L34", "uniref50_id": "UniRef50_P0A7P5", "cog_id": "COG0230", "cog_category": "J", "kegg_orthology_id": "K02914", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P0A7P5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0A7P5" ], "product": "Large ribosomal subunit protein bL34" }, "genes": [ "rpmH" ], "id": "KMADJIBOEG_1037", "locus": "GCA000292445_01037" }, { "type": "cds", "sequence": "contig_1", "start": 569483, "stop": 569878, "strand": "-", "frame": 3, "gene": "lolD", "product": "Putative ABC transporter ATP-binding protein MG467", "db_xrefs": [ "COG:COG1136", "COG:M", "GO:0005524", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P75110", "UniRef:UniRef90_P47705" ], "nt": "TTGTTAAGTGCTTATGATAATATTGCCATTTCACAAAACTTAATTCCCAAATATCAAAGACGACTTGATATAGAAGAGTTGGCTGAAAAATTAGATATTAAAGAAATCTTGTATAAATTTCCTTATGAGATGTCAGGTGGGCAAAAACAAAGAGTGGCCATCGCCCGTGCTATTATTAAAGAACCTAAACTATTACTTTGTGATGAACCTACTGGTGCACTTGATAGTAATTCTGCGGAAAATATCATTAATTTATTACAAACAATTAACAAAACATATAAGCAAACCATCTTAATGGTTACCCATGATGAAAGCCTAACAAGGATAGCTAATAGAATCATTAAAATTTCAGATGGTAAAATAGTATCTAACCAATTAGTCAGACCCTTAGTTTAG", "aa": "MLSAYDNIAISQNLIPKYQRRLDIEELAEKLDIKEILYKFPYEMSGGQKQRVAIARAIIKEPKLLLCDEPTGALDSNSAENIINLLQTINKTYKQTILMVTHDESLTRIANRIIKISDGKIVSNQLVRPLV", "aa_hexdigest": "b8f44a73be943d5c3953f018733a964b", "start_type": "TTG", "rbs_motif": null, "genes": [ "lolD" ], "truncated": "3-prime", "pseudogene": { "start": 569483, "stop": 570178, "observations": { "mutation": [ 570151 ], "stop": [ 570151 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47705", "query_cov": 1.0, "subject_cov": 0.4212218649517685, "identity": 0.985, "score": 248.0, "evalue": 8.6e-81, "gene_start": 1, "gene_end": 131, "reference_start": 181, "reference_end": 311, "reference_sequence": "MKKGNKTVWNECIDILDNVKSPSFSANFDDYFKKSKSKPPKKNKKVLNNIKKAELKLKKKANKKQKANTLYIPPFAQQAKGIVITINKMWKNVHNDDSKQEISILSDVSLQIAYGEIVIILGSSGSGKTTLLNLIGGYDSISLGSCIVANCPLEKCTSEQLLTYRKNNLGYVYQRYNLIELLSAYDNIAISQNLIPKYQRRLDIEELAEKLDIKEILYKFPYEMSGGQKQRVAIARAIIKEPKLLLCDEPTGALDSNSAENIINLLQTINKTYKQTILMVTHDVSLTRIANRIIKISDGKIVSNQLVRPLV" }, "paralog": false, "identity": 0.987012987012987, "score": 440.0, "evalue": 8.51e-159, "description": "Internal stop codon at 570151. Nonsense mutation around 570151.", "psc": { "uniref90_id": "UniRef90_P47705", "gene": "LolD", "product": "Putative ABC transporter ATP-binding protein MG467", "uniref50_id": "UniRef50_P75110", "cog_id": "COG1136", "cog_category": "M", "go_ids": [ "GO:0005524", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47705", "UniRef:UniRef50_P75110", "COG:COG1136", "COG:M", "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P75110", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75110" ], "product": "Putative ABC transporter ATP-binding protein MG467 homolog" } }, "id": "KMADJIBOEG_1038", "locus": "GCA000292445_01038" }, { "type": "cds", "sequence": "contig_1", "start": 570461, "stop": 570814, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATCAAGTAGATGCGTTTCTTTTAGGAACTATTATTCCTTTCATTTTCATTACTTGTGTTGTACTTGGTATCTCAATGTTAGAAGAGATGAAAAGAATCTTTATTTCACTTAAGGCTATTGGCTATAGAGATGTACAAAATTTAATTAGTTTACTTACCTTCTTTATCCCTGCATTTGTTTTAGCATTACTAATTTCTATTGGAGTTTTAGCTGGTGTTTTAATTGGTATCCAAGCAGTTGTTTTCAATGTTGCACAAGTCTTTTTAACTAATGTATTTGAGTTCTTGCCATACATGGTAGGGATAGTATTATTTGGTGTTACTATCTTTGTAATTGGTTCTTACTTTTGA", "aa": "MNQVDAFLLGTIIPFIFITCVVLGISMLEEMKRIFISLKAIGYRDVQNLISLLTFFIPAFVLALLISIGVLAGVLIGIQAVVFNVAQVFLTNVFEFLPYMVGIVLFGVTIFVIGSYF", "aa_hexdigest": "88d4140422110cf46f82bfa01e97becf", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12914.61039999999, "isoelectric_point": 4.935693550109861 }, "id": "KMADJIBOEG_1039", "locus": "GCA000292445_01039" }, { "type": "cds", "sequence": "contig_1", "start": 570950, "stop": 572671, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAGTAAACCTGTTAATGTCTTAATTGATCAAGGCAATGAGACCAAGTTTTCAGTTCTTCTTCATCTTTTAAAACATAAAAGTCATACAATGTCACCCTTGACCAAGTTTAGGGTTTCATTAATCGTTTCACGTTTTTCACGTTTATTTACCTATGTAGGATTGAGTTCAGTTGCACTTTTATTGATTGGGATTGCAGGTACTATTCCCCAAAAATTTAGTGCAGCGCAAACAAGCACTTCCTTAAATAGAAACTTTAACTATAAGTTGAATTTACAAACACCAACAGAGCAATCTGGATGGTATGCAATTCAACCCTATTCACACTTTGGAGTTACTGATAATAATAATGGTATTAAAACCTTGTATAACGAAAGTGTTCAAGCAAATTCTCAAAATGAGCACCCTTATAAACCAAGTAATTTAAAACTTAAAAACCGCCAGGATCAACCAATAAAAGCAGCAGATGGAACTGAGCTTGAATTGGGCAACTTACTTTTGCCTAGTTATGGTGGTGCTCAACAGTTAAGCACTGATGAGAATTTCTTTAGGCATGCCTCTCTATCAAAATGGATTATTGACTTTCCTATTAGGGTTGGCGGTTCTAACATTAACCCTTGGGAAATTGTTGAAAAATCAATTCCTAAACAGATTACTCAGTTACTTTCAGCATCTAGTGATCAGTTTTTAATTTCAGTATTAACTGATGACTTTTTCAATAACTTAAATGCTAATGGGTTTTTAATTAGAAACCCTAGAACTAATCAAATTCAGTTAGATGCTAGTAGGGTACTAACTACAATTGATGTATTTAATCCTGGTGGGGTTAAATTTAATGATAGCTTTCTTAGTTTTATGTTGAAAGTTTATGGGGATTTTGAACTTGCAAAACAAGATTCTAAGTTAAATTTTGGGATTGTTCCTGTTGACCCTGCCATAGAAGAAACCTACACTTATGTTGAAGGCCCATTTGGTTTTCAAGAAGATAATCTTGATGAGAATTCACCATACACTTTAACAGGGATTAACCCTGAATCAAGTTTTGTTAATCTGATAGATGGTAGTGGTAACTCTTTAAGAAATTTAATTAGTTCTGATCAGGAAATGAATGTAATTGTTAATGCCGGTTTTCAATATGCTAACAACATTAATATTGGTGATTATGTTTATATAAAACCGAAAAATACTGCCACAAGGTACTCTGAAAAATTTCTAAAAGCAACCTTAAATAATCCAACAGTAGCATTTAAAGTTGTTGGTGTTTCTACTGATGCTTTTGGACAAGAACTTTATATTAACCAACACATTGCTAATAACTTATTAAAACTAAGTGGTAATCAGGGCAGAGGTATTATTAGAGATGTTATTAAAAAGACAAATGGCCAATCTCAAAGTAATGAGGAATATGAAATTGATTATGTAAAGCCAAACGGTTATGTTCCTTTTAATGGTGTATTTTCCAAAGAATTAAAACCTTCTTTATTAAATAAGGCACTAGTATTAAACTCGATAATTGGTGTTTGGGGTAATTTTACTGACTTTGGTAATAATTTCCAAAATTTAGTAAGGAATAAACTTGATAAAGTTATTACATCCATACTACCTACTGATCCTGAAATTATTAATAAGTTAGCACAAGAAAAACAGATTATTAATACTACTTCAATGAATTATGAATCGCTTCGTAAAGAACTAGTTAATAAATACAAAACAGAATGA", "aa": "MFSKPVNVLIDQGNETKFSVLLHLLKHKSHTMSPLTKFRVSLIVSRFSRLFTYVGLSSVALLLIGIAGTIPQKFSAAQTSTSLNRNFNYKLNLQTPTEQSGWYAIQPYSHFGVTDNNNGIKTLYNESVQANSQNEHPYKPSNLKLKNRQDQPIKAADGTELELGNLLLPSYGGAQQLSTDENFFRHASLSKWIIDFPIRVGGSNINPWEIVEKSIPKQITQLLSASSDQFLISVLTDDFFNNLNANGFLIRNPRTNQIQLDASRVLTTIDVFNPGGVKFNDSFLSFMLKVYGDFELAKQDSKLNFGIVPVDPAIEETYTYVEGPFGFQEDNLDENSPYTLTGINPESSFVNLIDGSGNSLRNLISSDQEMNVIVNAGFQYANNINIGDYVYIKPKNTATRYSEKFLKATLNNPTVAFKVVGVSTDAFGQELYINQHIANNLLKLSGNQGRGIIRDVIKKTNGQSQSNEEYEIDYVKPNGYVPFNGVFSKELKPSLLNKALVLNSIIGVWGNFTDFGNNFQNLVRNKLDKVITSILPTDPEIINKLAQEKQIINTTSMNYESLRKELVNKYKTE", "aa_hexdigest": "31ee26cd49a919258b72afca5c83e4fa", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 64187.733900000065, "isoelectric_point": 7.076363563537597 }, "id": "KMADJIBOEG_1040", "locus": "GCA000292445_01040" }, { "type": "cds", "sequence": "contig_1", "start": 572687, "stop": 573460, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02687.27" ], "nt": "TTGCAAACACCAAACAATTTCCCTAACTGGTTAAATGATCTACCTGATCGTTATAAATTTAGTATTAACGGACTAACATTTGCAATTTTAGGTATAGGTGAATCAGTAGAAACTGGTTACCCAGTGTTATCTTTACAATCTCCGTTGCCTAATACCCAAGATGAAGCGTTAATCTTTGTTAATGATCAAGCTTACAGAAGTATTCTGTTTGCTGTACCAGCAGCTAACCAAGAAAATTATTATGCTTTTAAGTCAACAGATTTAAAACAGCACACTGACCAAGACCCAGTGCAGTTTATTGCTAATAGATTAGAAGGATATCTAGATGTTCCTAGAAGTGATTTAGCATTTAATGTTAAAGATATTTCTAAGTTTAATTATCTGACAACAGCAAGAAACTATTTTCCTGATTTAGTGCAAAGCTATTTGGCAATAGTAAGTACTGTTATTGCTATATTTTTGATTATTCTTGCTCTTTATTTAATTATTCTTTTAATTAAGTCATTTATTAAAAAGAACCAAACTGAGTTTTCTATTATCAGAGCAGGGGGATTTTCAACAACTAAATTTATTGTTGGAATGAGTGTGTTTGCTGGTATTGTTGCAATTGTTTCTAGTTTTTTAGGAGTTTTATTTGCCTTTCTTCTAGAAGGACAAGTAAAGGGAATCATAAATAGGTATTGGTTTATTGCTTTACCTGAAAATAGCTTTAACTGGTTATCGTTTTTTGGTTCTTTCTTTATAACCTTTTTTGTTTTTGAATTTATTAGTTGA", "aa": "MQTPNNFPNWLNDLPDRYKFSINGLTFAILGIGESVETGYPVLSLQSPLPNTQDEALIFVNDQAYRSILFAVPAANQENYYAFKSTDLKQHTDQDPVQFIANRLEGYLDVPRSDLAFNVKDISKFNYLTTARNYFPDLVQSYLAIVSTVIAIFLIILALYLIILLIKSFIKKNQTEFSIIRAGGFSTTKFIVGMSVFAGIVAIVSSFLGVLFAFLLEGQVKGIINRYWFIALPENSFNWLSFFGSFFITFFVFEFIS", "aa_hexdigest": "3a8d471eaf58702ca7def26266c0fc43", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02687.27", "name": "FtsX", "length": 108, "aa_cov": 0.42023346303501946, "hmm_cov": 0.9814814814814815, "evalue": 5.233710005574544e-11, "score": 37.818782806396484, "start": 149, "stop": 256 } ], "seq_stats": { "molecular_weight": 29132.26780000003, "isoelectric_point": 5.189138603210448 }, "id": "KMADJIBOEG_1041", "locus": "GCA000292445_01041" }, { "type": "cds", "sequence": "contig_1", "start": 573470, "stop": 573640, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGAGATCCCTATTACTGTTAAAAATGGGGTCAATCCATTAATATTACCTACTGATCAACAAAATTTAGTTGTGCAAACCCCTGAAGCACACGGAGCGGTTGTTTCTCAACAATGGTTGTTTAGACACAATAAAGAAATTCTTCCCCAGGAAGGGGAATATGCTTGA", "aa": "MLEIPITVKNGVNPLILPTDQQNLVVQTPEAHGAVVSQQWLFRHNKEILPQEGEYA", "aa_hexdigest": "de0f0ed0d6112401acaecc8ac71561fe", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6305.134699999999, "isoelectric_point": 5.013165092468261 }, "id": "KMADJIBOEG_1042", "locus": "GCA000292445_01042" }, { "type": "cds", "sequence": "contig_1", "start": 573779, "stop": 573928, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGCTAGTTTAAAAAATACCCAAGCTTTAAGTCCAAAATCTACTAAATTTCCCATTTATCCAAAGTTAGCAAATATTATTGCTCAAGCCCAATTACCAGAAGCTACTAATATCCCAACTACTGCTTTAGATGCTTTAAAACAATGA", "aa": "MTASLKNTQALSPKSTKFPIYPKLANIIAQAQLPEATNIPTTALDALKQ", "aa_hexdigest": "bcf5b4d866410aac7ef0c2d45bb43c5e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5253.076300000001, "isoelectric_point": 9.699606895446777 }, "id": "KMADJIBOEG_1043", "locus": "GCA000292445_01043" }, { "type": "cds", "sequence": "contig_1", "start": 574283, "stop": 574486, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGGTAAGAAATAATAATCTTTCTTTTCAACCTTCAAGCAACAATCTCCAATTAGTTCAAAATCAAAACAGTCAAGCAAGTAATGGAATAGCTGATCCTAACTTTTCAAATGTTCAAACTGCATACAATAAAATTCACCAGCCCAATAACACTCCTGAAAAAACCCTTGATGCAGTTTATGCAGCAGTACTTGATCAATGA", "aa": "MMVRNNNLSFQPSSNNLQLVQNQNSQASNGIADPNFSNVQTAYNKIHQPNNTPEKTLDAVYAAVLDQ", "aa_hexdigest": "d66d5819d222054a4e5f497609e4c792", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7419.027700000002, "isoelectric_point": 5.4106969833374015 }, "id": "KMADJIBOEG_1044", "locus": "GCA000292445_01044" }, { "type": "cds", "sequence": "contig_1", "start": 574509, "stop": 574769, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCTTTATCACTATCACTAAAACCTGGTAAGGAATTGAGAATTGTATCTTTCTCAGATTCAACTTTCTTTTTAAGTCAATCTTTATTAATTTTTAGTTCAAATTTCTTAAGTTGCTTAAGTTTATTTTCAAGATCTCAATCTCCAAAAGAGGAATTATTGGCTATAGTTTTAGCATTAGTTTCTAACCACTTTAGCTGTTCATTATCTAGCTTATTTAATTTAGAAATATCTAAATTAAAAGTTAATGTAAAACTTTGA", "aa": "MSLSLSLKPGKELRIVSFSDSTFFLSQSLLIFSSNFLSCLSLFSRSQSPKEELLAIVLALVSNHFSCSLSSLFNLEISKLKVNVKL", "aa_hexdigest": "335e368951636c6eb6485f6b20769a03", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9517.088699999991, "isoelectric_point": 9.184117698669432 }, "id": "KMADJIBOEG_1045", "locus": "GCA000292445_01045" }, { "type": "cds", "sequence": "contig_1", "start": 574898, "stop": 575773, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTCTTTTTTTAAACAGATATTTAAGTCGCTTAAAAAGTTCTTCTTTTTACTATTTGGGATTATCTTTGTTTTATTTAGCATTATTTTCCTTGAAACATCTATTGTTCAGTTAAGTAATAACTTAGTTAGTACCTACACTACATTAGTATCTAAAACTAACTCTTCTGATATAGTAGCTCCTGCTATTTTAAAGGAAGCTAATCCTGTTTATACAGCAAGTTTAACTAATGATAGTGGTTATTTTTCCAAAATAAAGATAGATGATAAAAAAATTAACTATCTTTTTCCTTATCAAGAAAATGATTTTGGAAGTGATTCAGGACAAAGTAATGGTAGTGAGGATAACCAAAACAAAACCATTCCCCGCAAAGGCGATGTTAATGAAAAAGATAAACTCTTTTTAGCAAGAAAAAGAGGTATTTTAAAAGCATATGGAGAAGCCAATATTGCTGAAAAAAGAATTTATAAAGGGTTAGCAGTTTCTTTTAATAATACTGATTCTTTTAATGGTAATGATATTAGTGATTCCATTTCAAATAGACACATAATATCAGACCCTCAAAACCTTATTTATGATGCTAGTGGTAACCTTCTAGGTTATTTTGCTGATGGTTTAATTAAAGAAACTATCTCACTGCGTGCTGGCATTGCGCGTTTTCCTGGTGATAAAGGAAAAAGCACAGGAACACAAATAAAAATTACACAAAAGCAACAAACAAACACTGATCCACAAAAAGACAATACTGTTAATTCACTTTATAAGACCAATAACAAAGATAAAGTTTGGTTTAAGAGTGATGAGACCAAAGCAGATAATACTGATATATCTGCTAACTATCTATTTACAGGTGGTAATGAAGCTGCTAACTGA", "aa": "MFSFFKQIFKSLKKFFFLLFGIIFVLFSIIFLETSIVQLSNNLVSTYTTLVSKTNSSDIVAPAILKEANPVYTASLTNDSGYFSKIKIDDKKINYLFPYQENDFGSDSGQSNGSEDNQNKTIPRKGDVNEKDKLFLARKRGILKAYGEANIAEKRIYKGLAVSFNNTDSFNGNDISDSISNRHIISDPQNLIYDASGNLLGYFADGLIKETISLRAGIARFPGDKGKSTGTQIKITQKQQTNTDPQKDNTVNSLYKTNNKDKVWFKSDETKADNTDISANYLFTGGNEAAN", "aa_hexdigest": "453d6316fcfa871cf7ffed4451dda0fe", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 32450.99150000001, "isoelectric_point": 8.910191535949707 }, "id": "KMADJIBOEG_1046", "locus": "GCA000292445_01046" }, { "type": "cds", "sequence": "contig_1", "start": 575785, "stop": 576633, "strand": "-", "frame": 1, "gene": "lolD", "product": "Putative ABC transporter ATP-binding protein MG468-1", "db_xrefs": [ "COG:COG1136", "COG:M", "GO:0005524", "GO:0016887", "SO:0001217", "UniRef:UniRef50_Q50316", "UniRef:UniRef90_Q9ZB70" ], "nt": "ATGGTTTTAAAAACAAAAGAGAATAAAAAGTTTGATATTTACTTAAAATCATCAGATTTTGCTGTTAGTAAAAAAGCATCTAAATTAATTAAAAAGTTAAATAAAAAGCACCCCAAAAGAAAATCACTTAATTCATTTGAAGCTAAAAAGTATGATATTTACTTCAAAGAAGTTTGTAAAGCTGTTACAAATGGCATTAACAACCAACTTATTTGTAACCACATTAATTTAAAAATTCTTCCAGGAGAATTTGTTGTTATTCTTGGTAAATCTGGTAGTGGTAAAACCAGCTTACTTTCTTTAATTTCCGCATTAGATCGACCAACTAGCGGCGATAGTTTTGTTTGTGGAACTAACACCATATGTTGTAGTGATGCAAAACTAACCGCGTTAAGAAATAAAAACGTTGGTTATATTTTTCAACAATATGGTTTATTGCGTGATCTTGACGTTGATGATAATATTAAGCTTGCTCTACCATTAAAAAAAAGGTTTAATAATAATCTTGAAGAATTATTAGAACGCTTGGAATTAAAAGAACATAGACATAAGAAAGTTCATAAACTCTCTGGTGGACAACAACAACGTGTTGCTATTGCTCGGGCTTTAATTAAAGAACCTAAAATTTTGTTTGGTGATGAACCTACTGGAGCAGTTAATATTGATATTTCCAAAAAAATATTGCAATTTTTTGTGGAATATAATCGTGACAAAGGTACTACTATAGTAATAGTTACCCACAACGAAAAGATAGTGGAATTAGCTAAACGGGTAATTAAAATCCATGATGGCAAGATAATCGTTGATTATTTAAATCAGAATCCAAAAACCATCGAACAAATTAACTGA", "aa": "MVLKTKENKKFDIYLKSSDFAVSKKASKLIKKLNKKHPKRKSLNSFEAKKYDIYFKEVCKAVTNGINNQLICNHINLKILPGEFVVILGKSGSGKTSLLSLISALDRPTSGDSFVCGTNTICCSDAKLTALRNKNVGYIFQQYGLLRDLDVDDNIKLALPLKKRFNNNLEELLERLELKEHRHKKVHKLSGGQQQRVAIARALIKEPKILFGDEPTGAVNIDISKKILQFFVEYNRDKGTTIVIVTHNEKIVELAKRVIKIHDGKIIVDYLNQNPKTIEQIN", "aa_hexdigest": "ef08384f9e865f7a80c59c3d5d757ed0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9ZB70", "query_cov": 1.0, "subject_cov": 0.9929577464788732, "identity": 1.0, "score": 542.0, "evalue": 1.64e-194, "valid": true, "gene": "LolD", "product": "Putative ABC transporter ATP-binding protein MG468.1", "uniref50_id": "UniRef50_Q50316", "cog_id": "COG1136", "cog_category": "M", "go_ids": [ "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_Q50316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50316" ], "product": "Putative ABC transporter ATP-binding protein MG468.1 homolog" }, "genes": [ "lolD" ], "id": "KMADJIBOEG_1047", "locus": "GCA000292445_01047" }, { "type": "cds", "sequence": "contig_1", "start": 576696, "stop": 578009, "strand": "-", "frame": 2, "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "db_xrefs": [ "COG:COG0593", "COG:L", "GO:0003688", "GO:0005524", "GO:0005737", "GO:0005886", "GO:0006260", "GO:0006270", "GO:0006275", "GO:0016887", "KEGG:K02313", "RefSeq:WP_010869493.1", "SO:0001217", "UniParc:UPI000012952C", "UniRef:UniRef100_P35888", "UniRef:UniRef50_P35888", "UniRef:UniRef90_P35888" ], "nt": "ATGGAACAATTTAATGCCTTTAAATCTCTATTAAAAAAGCATTATGAAAAAACAATAGGTTTTCATGATAAATACATTAAAGACATTAATCGTTTCGTATTTAAAAATAATGTTCTTTTAATTCTTTTAGAAAATGAATTTGCTCGTAATTCCTTAAATGATAATTCTGAAATTATTCATTTAGCTGAAAGTTTGTATGAAGGAATTAAAAGTGTTAATTTTGTTAATGAGCAAGATTTCTTTTTTAACTTAGCAAAATTAGAAGAAAATAGTCGTGATACTCTTTATCAAAATTCTGGATTGAGTAAAAACTATACTTTTCAAAACTTTGTAATTAGTGAAGGAAATAAAAGAGCTTATGAAGCAGGCGTTAGATTAGCTGAAACTCAAGATAACGAATTTTCACCGCTTTTTATTTACGGAGAAACCGGTCTTGGTAAAACTCACCTACTACAAGCAATAGGAAATGAAAAATTTCGTAATTTTCCAAATGCCAGAGTAAAGTATGTTGTTTCAAGTGATTTTGCCCAAGAAGTTGTTGATGCTTTTTATCAAAGGGATAAAGGTATAGAAAAACTAAAAAAAAATTATGAAAATTTAGATTTAGTTTTAATAGATGACACTCAAATATTTGGCAGAAAAGAAAAAACCTTAGAAATTCTTTTCAATATTTTTAATAACCTAGTTCTAAATAAAAAACAAATTGTTTTAGTTTCTGATAAGGCTCCTGATGAACTAATTGATATTGATGCAAGAATGATTTCTCGCTTTAAATCAGGATTATTACTAAAGATAGAAAAGCATAATTTGTCTTCACTTTGTGAAATACTTACTGTTAAATTAAAAGAAAAAGATCCTAACATCCAAATAACTAATGAGGCAAGACATGATGCAGCACAAATTTCAGGTAACGATGTGCGTGCTTTAAATGGAATTGCAACAAAGTTATTATTTTTTGCTAAAACTTCAAAACAAAATTTAATAAATACTGAAAATTTAAAAGAAATTCTTTTTGAAGAATTTGAGAAGTTTCATAAAAAGAGCTTTGATCCTTATTTATTAATAGAGAACGTTTGCCGTAGATTTAATGTTCCTATGGACAGTGTACTTTCAGAAAATCGTAAAGCAGAACTTGTCCGTGTTCGTGATGTGTGTAATTACCTTTTGCGTCAAAAGTACAACATGCAATTTCAACAAATTGGCAAAATATTTAAGAGAAGTCATTCAAGTGTATTAATGGCAGTTAAAAGAGTTGCTAAAATGATTGAAAATGACAGTTCATTACGGGATGTAATTACATCATTAGTAATTTAG", "aa": "MEQFNAFKSLLKKHYEKTIGFHDKYIKDINRFVFKNNVLLILLENEFARNSLNDNSEIIHLAESLYEGIKSVNFVNEQDFFFNLAKLEENSRDTLYQNSGLSKNYTFQNFVISEGNKRAYEAGVRLAETQDNEFSPLFIYGETGLGKTHLLQAIGNEKFRNFPNARVKYVVSSDFAQEVVDAFYQRDKGIEKLKKNYENLDLVLIDDTQIFGRKEKTLEILFNIFNNLVLNKKQIVLVSDKAPDELIDIDARMISRFKSGLLLKIEKHNLSSLCEILTVKLKEKDPNIQITNEARHDAAQISGNDVRALNGIATKLLFFAKTSKQNLINTENLKEILFEEFEKFHKKSFDPYLLIENVCRRFNVPMDSVLSENRKAELVRVRDVCNYLLRQKYNMQFQQIGKIFKRSHSSVLMAVKRVAKMIENDSSLRDVITSLVI", "aa_hexdigest": "78cb41d1b32b566578d71be19e91d6ce", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012952C", "ncbi_nrp_id": "WP_010869493.1", "uniref100_id": "UniRef100_P35888", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012952C", "RefSeq:WP_010869493.1", "UniRef:UniRef100_P35888" ] }, "ips": { "uniref100_id": "UniRef100_P35888", "uniref90_id": "UniRef90_P35888" }, "psc": { "uniref90_id": "UniRef90_P35888", "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "uniref50_id": "UniRef50_P35888", "cog_id": "COG0593", "cog_category": "L", "kegg_orthology_id": "K02313", "go_ids": [ "GO:0005737", "GO:0005886", "GO:0005524", "GO:0016887", "GO:0003688", "GO:0006260", "GO:0006270", "GO:0006275" ] }, "pscc": { "uniref50_id": "UniRef50_P35888", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P35888" ], "product": "Chromosomal replication initiator protein DnaA" }, "genes": [ "dnaA" ], "id": "KMADJIBOEG_1048", "locus": "GCA000292445_01048" }, { "type": "oriC", "sequence": "contig_1", "start": 578010, "stop": 578651, "strand": "?", "product": "origin of replication", "nt": "TATTCTTCTATAACATTGTCAAGAATGATAGTTAAAATTCTCGAAATTGGGATATTAACTGCTTTGGAGTAATTTCTAACTTTTTGTCATACTCTTTGACTTGTATAGAAGTGTACACCTGTATCTAGTTTTTCTTGGCGTTCAACAGGAACTATTCCTGGTATTTTTGTTTTAGGTTGGGGAGGAATAGGCTGTGGTTGTGTGAATTGTTGTTGAAAATTTTGATTTTTTTGCTGTAAGAAACCATTATTATGATATTGAAAATTTTGTTCCTCTTTAAAATATCTCTCTTTTTTTGGTTTTCCAGAAAAATTTGATGAAAAAGATTTTTCTTCATTTCAATTTTCAAGATTATTTTCATTTTGTTGATTTATTTGCTCAGGCTGTTGAAATGAATTATTTTTTGATCAAAAAGATTTTGGAAAGGTTTTTTCAAAAGCAGATAAAGGTCCAAAATCAAATGAAGATGAATCTTTGTCAAAAGATGTTTCTTCTCTTTTTGACAAATTTTGTTTTTGATTAAACTTATTTTTATTTTGGGGTGTTACTTTTTCTTTTATGGAAAACAAATCTTCTTCTAAAAGACTTTGTTCTGGGTCATCATCTTGTGCTAAATCAAAGAAAAAACGTTTCTTTTTGTTA", "id": "KMADJIBOEG_1051" }, { "type": "cds", "sequence": "contig_1", "start": 578417, "stop": 578662, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCCATTAATAACAAAAAGAAACGTTTTTTCTTTGATTTAGCACAAGATGATGACCCAGAACAAAGTCTTTTAGAAGAAGATTTGTTTTCCATAAAAGAAAAAGTAACACCCCAAAATAAAAATAAGTTTAATCAAAAACAAAATTTGTCAAAAAGAGAAGAAACATCTTTTGACAAAGATTCATCTTCATTTGATTTTGGACCTTTATCTGCTTTTGAAAAAACCTTTCCAAAATCTTTTTGA", "aa": "MSINNKKKRFFFDLAQDDDPEQSLLEEDLFSIKEKVTPQNKNKFNQKQNLSKREETSFDKDSSSFDFGPLSAFEKTFPKSF", "aa_hexdigest": "a134ba9314829478f9d26a2092170a99", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9534.465299999993, "isoelectric_point": 5.4164945602416985 }, "id": "KMADJIBOEG_1049", "locus": "GCA000292445_01049" }, { "type": "cds", "sequence": "contig_1", "start": 578652, "stop": 579461, "strand": "-", "frame": 2, "gene": "parA", "product": "ParA family protein MG470", "db_xrefs": [ "COG:COG1192", "COG:DN", "KEGG:K03496", "RefSeq:WP_009885563.1", "SO:0001217", "UniParc:UPI000013131F", "UniRef:UniRef100_P47706", "UniRef:UniRef50_P47706", "UniRef:UniRef90_P47706" ], "nt": "ATGATCATTTCTTTTGTAAATAATAAAGGCGGCGTATTAAAAACTACAATGGCAACTAATGTTGCAGGATCATTGGTTAAACTTTGTCCAGAAAGGCGAAAAGTCATTCTTGATCTTGACGGGCAGGGGAATGTTTCTGCATCATTTGGACAAAACCCTGAAAGATTGAATAACACGTTAATTGACATTTTGTTGAAGGTTCCAAAATTCAGTGGTTCAAATAATTTCATTGAAATAGATGATTGCTTGCTATCTGTATATGAGGGACTTGATATTCTTCCTTGTAATTTTGAACTTAATTTTGCTGATATAGACATATCACGTAAAAAATATAAGGCCTCAGATATAGCAGAAATAGTTAAGCAACTAGCAAAAAGATATGAATTTGTTTTATTAGATACACCACCAAATATGGCAACTTTAGTTTCTACTGCAATGAGTTTATCAGATGTCATAGTTATTCCCTTTGAACCAGATCAATATTCGATGCTAGGTTTGATGAGAATAGTTGAAACAATAGATACATTTAAGGAAAAAAATACAAATCTTAAAACTATTTTAGTTCCTACAAAAGTTAACGTTCGTACTCGTCTTCATAATGAAGTAATAGATTTAGCTAAAACAAAAGCTAAAAAAAATAATGTAGCTTTTTCTAAAAATTTTGTTTCTCTGACATCAAAATCATCAGCAGCTGTAGGTTATGAAAAATTGCCAATATCATTAGTATCATCACCAAGTAAAAAATATCTAAATGAGTATTTAGAAATAACAAAAGAAATCCTAAATTTAGCAAATTACAATGTCCATTAA", "aa": "MIISFVNNKGGVLKTTMATNVAGSLVKLCPERRKVILDLDGQGNVSASFGQNPERLNNTLIDILLKVPKFSGSNNFIEIDDCLLSVYEGLDILPCNFELNFADIDISRKKYKASDIAEIVKQLAKRYEFVLLDTPPNMATLVSTAMSLSDVIVIPFEPDQYSMLGLMRIVETIDTFKEKNTNLKTILVPTKVNVRTRLHNEVIDLAKTKAKKNNVAFSKNFVSLTSKSSAAVGYEKLPISLVSSPSKKYLNEYLEITKEILNLANYNVH", "aa_hexdigest": "e8f293204ff28c39358c46de4a80ed83", "start_type": "ATG", "rbs_motif": "TTATA", "ups": { "uniparc_id": "UPI000013131F", "ncbi_nrp_id": "WP_009885563.1", "uniref100_id": "UniRef100_P47706", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013131F", "RefSeq:WP_009885563.1", "UniRef:UniRef100_P47706" ] }, "ips": { "uniref100_id": "UniRef100_P47706", "uniref90_id": "UniRef90_P47706" }, "psc": { "uniref90_id": "UniRef90_P47706", "gene": "ParA", "product": "ParA family protein MG470", "uniref50_id": "UniRef50_P47706", "cog_id": "COG1192", "cog_category": "DN", "kegg_orthology_id": "K03496" }, "pscc": { "uniref50_id": "UniRef50_P47706", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47706" ], "product": "ParA family protein MG470" }, "genes": [ "parA" ], "id": "KMADJIBOEG_1050", "locus": "GCA000292445_01050" } ], "sequences": [ { "id": "contig_1", "description": "[organism=Mycoplasmoides genitalium] [strain=M6282] [gcode=11] [completeness=complete] [topology=circular]", "nt": "TAAGTTATTATTTAGTTAATACTTTTAACAATATTATTAAGGTATTTAAAAAATACTATTATAGTATTTAACATAGTTAAATACCTTCCTTAATACTGTTAAATTATATTCAATCAATACATATATAATATTATTAAAATACTTGATAAGTATTATTTAGATATTAGACAAATACTAATTTTATATTGCTTTAATACTTAATAAATACTACTTATGTATTAAGTAAATATTACTGTAATACTAATAACAATATTATTACAATATGCTAGAATAATATTGCTAGTATCAATAATTACTAATATAGTATTAGGAAAATACCATAATAATATTTCTACATAATACTAAGTTAATACTATGTGTAGAATAATAAATAATCAGATTAAAAAAATTTTATTTATCTGAAACATATTTAATCAATTGAACTGATTATTTTCAGCAGTAATAATTACATATGTACATAGTACATATGTAAAATATCATTAATTTCTGTTATATATAATAGTATCTATTTTAGAGAGTATTAATTATTACTATAATTAAGCATTTATGCTTAATTATAAGCTTTTTATGAACAAAATTATAGACATTTTAGTTCTTATAATAAATAATAGATATTAAAGAAAATAAAAAAATAGAAATAAATATCATAACCCTTGATAACCCAGAAATTAATACTTAATCAAAAATGAAAATATTAATTAATAAAAGTGAATTGAATAAAATTTTGAAAAAAATGAATAACGTTATTATTTCCAATAACAAAATAAAACCACATCATTCATATTTTTTAATAGAGGCAAAAGAAAAAGAAATAAACTTTTATGCTAACAATGAATACTTTTCTGTCAAATGTAATTTAAATAAAAATATTGATATTCTTGAACAAGGCTCCTTAATTGTTAAAGGAAAAATTTTTAACGATCTTATTAATGGCATAAAAGAAGAGATTATTACTATTCAAGAAAAAGATCAAACACTTTTGGTTAAAACAAAAAAAACAAGTATTAATTTAAACACAATTAATGTGAATGAATTTCCAAGAATAAGGTTTAATGAAAAAAACGATTTAAGTGAATTTAATCAATTCAAAATAAATTATTCACTTTTAGTAAAAGGCATTAAAAAAATTTTTCACTCAGTTTCAAATAATCGTGAAATATCTTCTAAATTTAATGGAGTAAATTTCAATGGATCCAATGGAAAAGAAATATTTTTAGAAGCTTCTGACACTTATAAACTATCTGTTTTTGAGATAAAGCAAAAAACAGAACCATTTGATTTCATTTTGGAGAGTAATTTACTTAGTTTCATTAATTCTTTTAATCCTGAAGAAGATAAATCTATTGTTTTTTATTACAGAAAAGATAATAAAGATAGCTTTAGTACAGAAATGTTGATTTCAATGGATAACTTTATGATTAGTTACACATCGGTTAATGAAAAATTTCCAGAGGTAAACTACTTTTTTGAATTTGAACCTGAAACTAAAATAGTTGTTCAAAAAAATGAATTAAAAGATGCACTTCAAAGAATTCAAACTTTGGCTCAAAATGAAAGAACTTTTTTATGCGATATGCAAATTAACAGTTCTGAATTAAAAATAAGAGCTACTGTTAATAATATCGGAAATTCTCTTGAGGAAATTTCTTGTCTTAAATTTGAAGGTTATAAACTTAATATTTCTTTTAACCCAAGTTCTCTATTAGATCACATAGAGTCTTTTGAATCAAATGAAATAAATTTTGATTTCCAAGGAAATAGTAAGTATTTTTTGATAACCTCTAAAAGTGAACCTGAACTTAAGCAAATATTGGTTCCTTCAAGATAATGAATCTTTACGATCTTTTAGAACTACCAACTACAGCATCAATAAAAGAAATAAAAATTGCTTATAAAAGATTAGCAAAGCGTTATCACCCTGATGTAAATAAATTAGGTTCGCAAACTTTTGTTGAAATTAATAATGCTTATTCAATATTAAGTGATCCTAACCAAAAGGAAAAATATGATTCAATGCTGAAAGTTAATGATTTTCAAAATCGCATAAAAAATTTAGATATTAGTGTTAGATGACATGAAAATTTCATGGAAGAACTCGAACTTCGTAAGAACTGAGAATTTGATTTTTTTTCATCTGATGAAGATTTCTTTTATTCTCCATTTACAAAAAACAAATATGCTTCCTTTTTAGATAAAGATGTTTCTTTAGCTTTTTTTCAGCTTTACAGCAAGGGCAAAATAGATCATCAATTGGAAAAATCTTTATTGAAAAGAAGAGATGTAAAAGAAGCTTGTCAACAGAATAAAAATTTTATTGAAGTTATAAAAGAGCAATATAACTATTTTGGTTGAATTGAAGCTAAGCGTTATTTCAATATTAATGTTGAACTTGAGCTCACACAGAGAGAGATAAGAGATAGAGATGTTGTTAACCTACCTTTAAAAATTAAAGTTATTAATAATGATTTTCCAAATCAACTCTGATATGAAATTTATAAAAACTATTCATTTCGCTTATCTTGAGATATAAAAAATGGTGAAATTGCTGAATTTTTCAATAAAGGTAATAGAGCTTTAGGATGAAAAGGTGACTTAATTGTCAGAATGAAAGTAGTTAATAAAGTAAACAAAAGACTGCGTATTTTTTCAAGCTTTTTTGAGAACGATAAATCTAAATTATGGTTCCTTGTTCCAAACGATAAACAAAGTAATCCTAATAAGGGCGTTTTTAACTATAAAACTCAGCACTTTATTGATTAAAAAACCTTTCATTTTTAATGTGTTATAATTATTTGTTATGCCATAAATTTAGTTTGTGGCAAAAGCTTCTGTACTGTTTATTTAATGGAAGAAAATAACAAAGCAAATATCTATGACTCTAGTAGCATTAAGGTCCTTGAAGGACTTGAGGCTGTTAGAAAACGCCCTGGAATGTACATTGGTTCTACTGGCGAAGAAGGTTTGCATCACATGATCTGAGAGATAGTAGACAACTCAATTGATGAAGCAATGGGAGGTTTTGCCAGTTTTGTTAAGCTTACCCTTGAAGATAATTTTGTTACCCGTGTAGAGGATGATGGAAGAGGGATACCTGTTGATATCCATCCTAAGACTAATCGTTCTACAGTTGAAACAGTTTTTACAGTTCTACACGCTGGCGGTAAATTTGATAACGATAGCTATAAAGTGTCAGGTGGTTTACACGGTGTTGGTGCATCAGTTGTTAATGCGCTTAGTTCTTCTTTTAAAGTTTGAGTTTTTCGTCAAAATAAAAAGTATTTTCTCAGCTTTAGCGATGGAGGAAAGGTAATTGGAGATTTGGTCCAAGAAGGTAACTCTGAAAAAGAGCATGGAACAATTGTTGAGTTTGTTCCTGATTTCTCTGTAATGGAAAAGAGTGATTACAAACAAACTGTAATTGTAAGCAGACTCCAGCAATTAGCTTTTTTAAACAAGGGAATAAGAATTGACTTTGTTGATAATCGTAAACAAAACCCACAGTCTTTTTCTTGAAAATATGATGGGGGATTGGTTGAATATATCCACCACCTAAACAACGAAAAAGAACCACTTTTTAATGAAGTTATTGCTGATGAAAAAACTGAAACTGTAAAAGCTGTTAATCGTGATGAAAACTACACAGTAAAGGTTGAAGTTGCTTTTCAATATAACAAAACATACAACCAATCAATTTTCAGTTTTTGTAACAACATTAATACTACAGAAGGTGGAACCCATGTGGAAGGTTTTCGTAATGCACTTGTTAAGATCATTAATCGCTTTGCTGTTGAAAATAAATTCCTAAAAGATAGTGATGAAAAGATTAACCGTGATGATGTTTGTGAAGGATTAACTGCTATTATTTCCATTAAACACCCAAACCCACAATATGAAGGACAAACTAAAAAGAAGTTAGGTAATACTGAGGTAAGACCTTTAGTTAATAGTGTTGTTAGTGAAATCTTTGAACGCTTCATGTTAGAAAACCCACAAGAAGCAAACGCTATCATCAGAAAAACACTTTTAGCTCAAGAAGCGAGAAGAAGAAGTCAAGAGGCTAGGGAGTTAACTCGTCGTAAATCACCTTTTGATAGTGGTTCATTACCAGGTAAATTAGCTGATTGTACAACCAGAGATCCTTCGATTAGTGAACTTTACATTGTTGAGGGTGATAGTGCTGGTGGCACTGCTAAAACAGGAAGAGATCGTTATTTTCAAGCTATCTTACCCTTAAGAGGAAAGATTTTAAACGTTGAAAAATCTAACTTTGAACAAATCTTTAATAATGCAGAAATTTCTGCATTAGTGATGGCAATAGGCTGTGGGATTAAACCTGATTTTGAACTTGAAAAACTTAGATATAGCAAGATTGTGATCATGACAGATGCTGATGTTGATGGTGCACACATAAGAACACTTCTCTTAACTTTCTTTTTTCGCTTTATGTATCCTTTGGTTGAACAAGGCAATATTTTTATTGCTCAACCCCCACTTTATAAAGTGTCATATTCCCATAAGGATTTATACATGCACACTGATGTTCAACTTGAACAGTGAAAAAGTCAAAACCCTAACGTAAAGTTTGGGTTACAAAGATATAAAGGACTTGGAGAAATGGATGCATTGCAGCTGTGAGAAACAACAATGGATCCTAAGGTTAGAACATTGTTAAAAGTTACTGTTGAAGATGCTTCTATTGCTGATAAAGCTTTTTCACTGTTGATGGGTGATGAAGTTCCCCCAAGAAGAGAATTTATTGAAAAAAATGCTCGTAGTGTTAAAAACATTGATATTTAATTTGGTTAGTATAAATGGCAAAGCAACAAGATCAAGTAGATAAGATTCGTGAAAACTTAGACAATTCAACTGTCAAAAGTATTTCATTAGCAAATGAACTTGAGCGTTCATTCATGGAATATGCTATGTCAGTTATTGTTGCTCGTGCTTTACCTGATGCTAGAGATGGACTTAAACCAGTTCATCGTCGTGTTCTTTATGGTGCTTATATTGGTGGCATGCACCATGATCGTCCTTTTAAAAAGTCTGCGAGGATTGTTGGTGATGTAATGAGTAAATTCCACCCTCATGGTGATATGGCAATATATGACACCATGTCAAGAATGGCTCAAGACTTTTCATTAAGATACCTTTTAATTGATGGTCATGGTAATTTTGGTTCTATAGATGGTGATAGACCTGCTGCACAACGTTATACAGAAGCAAGATTATCTAAACTTGCAGCAGAACTTTTAAAAGATATTGATAAAGATACAGTTGACTTTATTGCTAATTATGATGGTGAGGAAAAAGAACCAACTGTTCTACCAGCAGCTTTCCCTAACTTACTTGCAAATGGTTCTAGTGGGATTGCAGTTGGAATGTCAACATCTATTCCTTCCCATAATCTCTCTGAATTAATTGCGGGTTTAATCATGTTAATTGATAATCCTCAATGCACTTTTCAAGAATTATTAACTGTAATTAAAGGACCTGATTTTCCAACAGGAGCTAACATTATCTACACAAAAGGAATTGAAAGCTACTTTGAAACAGGTAAAGGCAATGTAGTAATTCGTTCTAAAGTTGAGATAGAACAATTGCAAACAAGAAGTGCATTAGTTGTAACTGAAATTCCTTACATGGTTAACAAAACTACCTTAATTGAAAAGATTGTAGAACTTGTTAAAGCTGAAGAGATTTCAGGAATTGCTGATATCCGTGATGAATCCTCTCGAGAAGGAATAAGGTTAGTGATTGAAGTAAAACGCGACACTGTACCTGAAGTTTTATTAAATCAACTTTTTAAATCAACAAGATTACAAGTACGCTTCCCTGTTAATATGCTTGCTTTAGTTAAAGGAGCTCCTGTACTTCTCAACATGAAACAAGCTTTGGAAGTATATCTTGATCATCAAATTGATGTTCTTGTTAGAAAAACAAAGTTTGTGCTTAATAAACAACAAGAACGTTATCACATTTTAAGCGGACTTTTAATTGCTGCTTTAAATATTGATGAGGTTGTTGCAATTATTAAAAAATCAGCAAATAACCAGGAAGCAATTAATACATTAAATACAAAGTTTAAGCTTGATGAAATTCAAGCTAAAGCAGTTCTTGACATGCGTTTAAGGAGCTTAAGCGTACTTGAAGTTAACAAACTTCAAACTGAACAAAAAGAGTTAAAAGATTCAATTGAATTTTGTAAGAAAGTGTTAGCTGATCAAAAATTACAGCTAAAAATAATCAAAGAGGAATTGCAAAAAATCAATGATCAGTTTGGTGATGAAAGAAGAAGTGAAATTCTCTATGATATCTCTGAGGAAATTGATGATGAATCATTGATAAAAGTTGAGAATGTAGTGATAACTATGTCTACAAATGGTTATCTAAAAAGGATTGGAGTTGATGCTTATAATCTTCAACATCGTGGTGGAGTTGGGGTTAAAGGGCTAACTACTTATGTTGATGATAGTATTAGTCAATTATTGGTCTGTTCAACTCACTCTGACTTATTATTTTTTACTGATAAGGGTAAGGTTTATAGAATTAGAGCTCATCAAATTCCCTATGGTTTTAGAACAAATAAAGGTATTCCCGCTGTTAACTTAATCAAAATTGAAAAGGATGAAAGAATTTGTTCATTGTTATCTGTTAATAACTATGATGATGGTTATTTCTTTTTCTGTACTAAAAATGGAATTGTTAAAAGAACGAGCTTGAATGAATTCATCAACATCTTAAGTAATGGTAAGCGGGCTATATCTTTTGATGATAATGACACTTTGTATTCAGTAATTAAAACCCACGGAAATGATGAGATTTTTATTGGTTCTACCAATGGATTTGTTGTTCGATTCCATGAAAATCAACTCAGAGTTCTTTCAAGAACAGCAAGAGGTGTATTTGGTATCAGTTTAAATAAAGGAGAATTTGTTAATGGACTATCAACTTCAAGCAACGGTAGCTTACTTTTATCAGTCGGTCAAAATGGAATAGGTAAATTAACGAGCATAGATAAATATAGGCTCACAAAACGTAATGCTAAGGGAGTTAAAACTCTAAGGGTTACTGATAGAACAGGCCCTGTTGTTACAACAACCACTGTTTTTGGTAATGAGGATCTTTTAATGATTTCCTCTGCTGGTAAAATTGTGCGTACCAGTTTACAAGAACTTTCAGAACAAGGTAAAAACACTTCTGGTGTTAAGTTAATTAGATTAAAAGATAATGAACGTTTAGAAAGAGTAACTATCTTTAAAGAAGAGTTAGAAGACAAAGAAATGCAACTAGAAGATGTTGGATCCAAACAAATTACGCAATAACTATGATTTCTTTAAAAAGAAACTGTTAGAAAGAAATGTAAATGAGCAATTATTAAATCAGTTTATTCAAACTGATAAACTAATGCGCAAAAACTTACAACAACTTGAACTTGCTAACCAAAAACAAAGCTTGTTGGCAAAACAAGTTGCTAAGCAAAAAGATAATAAAAAGCTATTAGCTGAATCAAAAGAACTTAAGCAGAAGATTGAAAACTTAAATAGTGCTTATAAAGATTCACAAAACATTAGTCAGGATTTACTTCTAAATTTTCCTAATATTGCTCATGAATCAGTTCCTGTTGGTAAAAATGAATCAGCAAACTTAGAACTCCTTAAAGAAGGGAGAAAACCAGTTTTTGATTTCAAACCTTTACCACATTGGGAGTTATGTGAAAAGTTAAATTTAGTTGCTTTTGATAAAGCTACTAAGATTAGTGGAACTAGGTTTGTTGCATATACAGATAAAGCAGCTAAACTGCTTAGAGCGATAACTAATCTAATGATTGACCTTAATAAAAGCAAGTATCAAGAATGAAACCTGCCAGTTGTTATTAATGAATTAAGTTTAAGATCAACCGGACAACTACCTAAGTTTAAAGATGATGTTTTTAAACTAGAAAACACCCGTTATTATCTTTCTCCAACTTTAGAGGTACAACTTATCAATTTACATGCTAATGAAATTTTTAATGAAGAAGATTTACCTAAATACTACACTGCAACAGGTATTAACTTTCGTCAAGAAGCGGGTAGTGCTGGTAAACAAACCAAAGGAACTATTAGATTGCATCAGTTTCAAAAAACTGAGTTAGTTAAGTTTTGTAAACCTGAAAATGCTATCAATGAATTGGAAGCAATGGTTAGAGATGCTGAACAAATCTTAAAGGCACTTAAGTTACCTTTTAGAAGGTTATTGTTATGTACTGGTGATATGGGCTTTAGTGCTGAAAAAACATATGATCTTGAAGTTTGAATGGCAGCTAGCAATGAATATCGTGAAGTTTCTTCTTGTTCATCTTGTGGTGATTTTCAAGCAAGAAGAGCTATGATTCGTTACAAAGATATTAACAACGGTAAAAACAGTTATGTTGCTACTTTAAATGGAACAGCATTATCTATTGATAGAATTTTTGCTGCAATTCTAGAAAATTTTCAAACAAAAGATGGCAAAATTCTTATCCCACAAGCATTAAAAAAATACCTTGATTTTGACACAATCAAGTAATTTATGAATAAAGGTGTTTTTGTTGTTATTGAAGGAGTTGATGGAGCGGGCAAAACTGCTTTAATTGAAGGTTTTAAAAAACTTTATCCAACTAAGTTTTTGAACTATCAACTTACTTATACTAGAGAACCTGGTGGTACTTTGTTAGCTGAAAAAATTCGTCAACTTCTTTTAAATGAAACAATGGAACCTCTAACTGAAGCTTATTTGTTTGCCGCAGCTAGAACTGAACATATCAGTAAGCTAATTAAACCAGCAATTGAAAAAGAACAACTAGTTATTTCAGATAGATTTGTTTTCTCTAGTTTTGCATACCAAGGATTAAGCAAAAAAATAGGCATTGATACAGTAAAACAGATTAATCATCATGCGTTAAGAAATATGATGCCAAACTTTACCTTTATTTTGGATTGCAATTTTAAAGAAGCATTACAAAGGATGCAAAAGCGTGGTAATGATAATCTTCTTGATGAATTTATTAAAGGAAAGAATGATTTTGATACAGTTCGTTCTTATTATTTAAGCTTAGTTGATAAAAAAAACTGTTTCTTGATTAATGGTGATAATAAACAAGAACACCTAGAGAAATTTATTGAATTGTTAACAAGATGCTTACAACAACCCACGCATTACTAATCATTCAAAGAAAAGGTAGTTTCTTAAAACCTTTTCTTGATAATTATCTTACTAGTATTGTTTGTGAAAACAAAAATGGTTGCAAAAAGTGTATAAACTGTTTGGAAATTCTCAATAATAAATACAACAGCTTATATTGATTTGATCAAATTAATCCTTTCAAAAGAGAAAATGCCCTTCAGTTAGCAAGAATTTTTAACCGTGAAAGAACAAGTGTAAACAATAAAAATATTTATCTAATTGAAGAAATTGAAAAATTAAGCAGTAATTCTATAAATAGTTTATTGAGACTAGTTGAAGATAGTCCGATAAATAGTTATGGTATTTTTACAACTAAAAATGAAAGTTTAATTCTTTCCACTTTTTTAAGTAGAGTACAGAAAGTAGTTTTAAAAAAAGCTAGTAAAGTTCCTTTTAAAGTAAGCAAAAATGATCAAGAAATTATTACAAGTTTTTTTACTGTAGATGAACAAATTGAAGCAATTGAAAATGGAAGTTTTAACCGTTTCAAAATTATCTTAGATGCATGTTTAAACAAAAAAACAGGTACAGAACAAATTTATCATGCTTGACAAATTTTTAGAGATTTTTCTAATAGTGAAATTGCTCAGTTAATTACTCTAATAATTAATAAAACTGAAAATATAGATAAAAAATCAATTTTGTTTAATTGTTTAAAAGTTTTGCCATATAATCCTCCAAAATCCACTTTGTTTGCTAATTTAGTTAGTTGATAGTTATGAAAAGCGAAATTAATATTTTTGCACTAGCAACTGCACCTTTTAATAGTGCATTACATATTATTAGGTTTTCTGGTCCTGATGTTTATGAGATTTTAAACAAGATAACTAATAAAAAAATAACAAGAAAAGGGATGCAAATTCAACGCACATGGATAGTTGATGAAAACAATAAGCGAATTGATGATGTGCTATTATTTAAATTTGTCTCTCCAAATTCTTATACAGGAGAAGATTTAATTGAAATTTCTTGTCATGGTAACATGTTGATCGTTAATGAAATTTGCGCACTTCTTTTAAAAAAAGGAGGTGTTTATGCCAAACCTGGTGAATTTACCCAAAGGAGTTTTTTAAATGGAAAAATGAGTTTACAACAAGCTAGTGCTGTAAATAAATTGATTTTATCTCCTAACTTATTAGTTAAAGATATAGTCTTAAATAATTTAGCGGGTGAAATGGATCAACAATTAGAACAAATAGCTCAACAAGTTAATCAATTAGTAATGCAAATGGAAGTAAACATTGATTATCCAGAATATCTTGATGAACAAGTAGAACTATCAACTTTAAATAATAAAGTTAAATTGATTATTGAAAAGCTTAAAAGAATTATTGAAAATAGTAAACAACTCAAAAAACTTCACGATCCTTTTAAAATTGCCATTATAGGCGAAACTAATGTAGGTAAATCTTCTTTACTCAACGCTTTATTAAATCAAGATAAAGCGATAGTTTCAAATATTAAAGGTAGTACACGCGATGTTGTTGAAGGGGATTTCAATTTAAATGGTTATTTAATCAAGATCTTAGATACTGCAGGTATCCGTAAACATAAAAGTGGGCTTGAAAAAGCAGGAATTAAAAAAGCTTTGAATCTATAAAGCAAGCTAATTTGGTTATTTATCTTTTAGATGCAACACATCCAAAGAAAGATCTTGAATTAATTAGTTTTTTTAAGAAAAATAAAAAGGATTTTTTTGTTTTCTATAACAAAAAAGATTTAATTACAAATAAGTTTGAAAATAGTATTTCTGCAAAGCAAAAAGATATTAAAGAATTAGTTAATTTATTAACTAAATATATTAACGAGTTTTATAAAAAAATAGATCAAAAAATCTATCTGATTGAAAATTGACAGCAAATTTTAATTGAAAAAATTAAAGAACAATTAGAACAGTTTTTAAAGCAACAAAAAAAATATTTATTTTTCGATGTTTTAGTTACCCATCTAAGAGAAGCTCAACAAGATATTCTTAAACTACTAGGTAAGGATGTAGGTTTTGATTTAGTTAATGAAATTTTTAATAATTTTTGTTTAGGAAAATAATGGAATACTTTGATGCACATTGTCATTTAAATTGTGAACCTTTACTGAGTGAAATTGAAAAAAGCATCGCTAATTTCAAATTAATTAATTTAAAAGCAAATGTTGTAGGTACAGATTTGGATAATTCTAAAATTGCTGTTGAATTAGCTAAAAAATATCCTGATCTTTTAAAAGCAACCATAGGTATCCATCCAAATGATGTTCATTTAGTTGATTTTAAAAAGACAAAAAAACAACTTAATGAACTATTAATAAATAACAGAAATTTCATAAGTTGTATTGGTGAATATGGTTTTGATTATCACTACACAACAGAATTTATTGAATTGCAAAACAAATTCTTTGAGATGCAATTTGAAATAGCTGAAACTAATAAATTGGTTCACATGCTTCATATTCGTGATGCTCATGAAAAAATTTATGAAATATTAACAAGATTAAAGCCAACTCAACCTGTGATTTTTCATTGTTTCAGTCAAGATATAAATATTGCTAAAAAGCTACTATCATTAAAAGATTTAAATATTGACATCTTCTTTTCTATCCCAGGGATAGTTACTTTTAAGAATGCTCAAGCATTACATGAAGCTTTAAAGATTATTCCTAGTGAATTACTTTTAAGTGAAACTGACTCACCGTGATTAACCCCTTCTCCTTTTCGAGGCAAAGTTAACTGACCTGAATATGTAGTTCATACTGTTAGCACTGTTGCTGAAATAAAAAAAATAGAAATTGCTGAAATGAAGCGAATTATTGTTAAAAATGCAAAAAAATTATTTTGACATTAAAAGTTAAATAAAGCAATTTATTTAACAAATGGATGTTAGAACTGAAAGATTAAACGAATTGTTTTTTGTTTATCATAAAAACTTAAAAAATCAATCTAAATCTAAATATAGCAGAGCAGTTAATTACTTAAAAAGGCGTGGATTTAATTTACAAGATTTTTTAAAAGTTGGGGGTGGTGTTGGTTATTTACAAAATAAAGAATGATTAAATTTACCTTTATACAGTTTTGATGGTAATTTAATTGGTTTTCTAAACAGAAAAGTTAGTTATAAAAAGGAATTTTTATATACACCTTTTAATAAACCTCCTTCAAAGAGCGAAGCTTTTGTAGGACTCAGAGAATTAGTTATTAAAGACAATAGCATATATCTTGTTGAAGGTGATTTTGACTGGTTAGCTTTTCGCAAAGCAGGTATATTAAATTCTCTTCCTCTTTGTGGTTTGACTATTTCAAATCAACAAGTTCAATGATTAAAACAAAAAAGATTAAAAAGATTTTTATCTGTTTTGATAATGATTTAGCTGGAAAGAATGGAGCAAAAAATTTAAAAGAATATCTAACTAAGCAAGGATTTATAACAAAAGTTATAGAAATTAAAGCTGCCGCAAAAGACTGAAATGATTTGTTTTTATTAAACAACTCAAATTGATCAGCGGTTTTAACTAATCAACTTCTTTTTTAAGAATTTTTTAAATTTACTAATAATTGTTCTGATAATTATTTTAGTGATATTTAAATCTGGACAAAGCTGAACTAAAGCTCTCGCACCAGCAGCATCTTCAATTTCATTAACAATAACCCTATTATATCTACTTAAAAAGAAGTCAATAGCATAATAACCTTCCCTTAGGCGTTTAGCTATTTTCTTTATTTTTCTTTTAGTAAATCACTTTAATTTAAACAAGGAAACTTCAGCACCTTGTGAAAAGTTAGCTTTAAATTGATTAGCATTAGAAATTTTTTTAATAACTTTAATTATTTTTCCAAACAAAATATAAGCACGATATTCAACTGTGCCAATTGATAAAAAAGGTTGAACAATTCATTCTGTTGCATTTTCAATGTTTAAATGTTTGATCTCGTCAGCACTATTAACTAAATGTACATCTTTTCCACCGTGTGAATTACGTTTCTTAACGATGGCAGGAAATGATTTGATTGTTTCTTTACTAAGAAGAGAAGAATTGACAGTTAGAAATCTATTTTGTTTTAATCATTTATATGTTTCGTATTTATCGTTTGCTATAAAAACAACATTAAAAGGATTAACTAAAAAAGTATTTATTTGATTATTGGTTTTTAAAAAATCTACTTTTCTTGAACGATTTAAAATCAATTCAGCTTCATTAATTTTAGCTTCGAAATTAATGTCTTTATTTTCAAGTAATAAGACTTCAGCATTTAGTTTTTCTTTTGTAATTCCTTGATTAGACTTAAAGCAAATGTTTGATTTTTTTCAAAATCATCCTTGTTGTCATAAACAACTAATGCTTTTCTGTTTTTTAATTTAATTTTTCCCATTAATCTAAATTGCTTTTAAAAGCTCAATTGCAAGATTAGTATTTAAATACATTGAGCTTCTTGTTAATTGCACATTAGGATTTACTTCACAAAAGATCAATGATCTGTCTTGATCAAACAAAAAATCAATACCGCAATAAAAAAGTTGCATTACTTTACTAATTTTAACTGCTAAATTTTCTTGTTCCTTATTCAAAAAAAAGCGTTCTGCCTTTGCCCCTTTATTGAGATTAGAACGAAAATCACTATTATTAGTTGTATGTAAAGCACCTATAACTTTATTGTTCACAACAATAACTCTTACCGATTCACCTTTAGAGCAAGTAATGTATTTTTGCACAATTGCTTGTTGATTAAAAGACATTAAAGTTTTTCTTAATTTTTGTTCATCTAAACACAAAAAAACATAATCACCAAAAGAACCAAAAACACTTTTAACAATAACAGGAAACTCCATACTTTGTTTTATTTGATTAATAAAAACATCGAGCATTACTGGATTTCACTCCCTGTCAAAATTTTGAGGTCCTAAAAGTGTTTTAATCTGCTTTATGAATGGATATTGTGCAATGATAGCGTGACTAAGTCCTTTATTATCTGCATTGTTAATTCCAATTGAACTGTTAATTACTTCAAAACCATTGCTTTCTAGTCATCTAGCTAAAGCAATATTTTTATCCATAAAAAGAATTTTGTTGGATATGAAGGGTAAATTTGCTTTTGCTTTAGTTGTATTAATATCAAAACTAGTAAAGAATTTTAGTTCTATATCTAACTCTTCACTAGCATTTTTTAAAAGTTGAGTTTGATTTAATTTAGAGCTAATTTGATTAAATGCTGGATTGTAAACAACGTTTATTTTTTTCATGCAATATTCAAAGATTTTTTATTATTGCTAAAACTGTAAAAGGGCCAACACCGCCAGGCGTTTTAGTTCATCATCTAGCTTTTTTTTCAATGCCTTCAGGATTTATATCACCACAAAGTTTATTTTGCTTGTTTCTTGAAACACCTATATCAAATAAAATAACTCCTTTTTTAAAGTTTTTAGCTTGAAAAAAATGAGATTTTCCAATAGCAGTAAAAACTATATCAGCACTTTTAATCAATTCAAATGTATTTGGAGTATCTTTATCGCATGCTTGAACTTTATAACCACTAGTTTTTAAAAATTCATAAATAGGTTTGCCACCAGTTATTCCCAAACCTACCACAACAATTTTTTGATTATTAAATTCAAGGTTAAATAGTTCTTTTAGTGTTATTATAGCTTCAACTATACAAGGTTTAATTTCAGCCAAATGATTAGTTGTTAAACCATCAACATCTTTTAAAGGATTAATTGCTTCTAGTAGTGAGTTTTTATCTAAGTGTGGATAAACTGGTAATTGCAAAATGATACCAGTAACATTTACATCATGATTTAAACTAATTATTTTTTCTATTAACTCTGCTTGAGTTATTTGATTAGATAGTTTAATTAATTCACTTTTTGCTCCTACAGTGTTACAAGCAATAAGTTTTTGTTTAATAAAACTGTCTGATGAATCATCATCATTAGCTTGAATAATCACTAACTTACATTTTGATCAATCATGCTTCTTGTAAGTTTCTAAGATTGATTGCGCTTTTAGTTTTCCATCAAATGACATCTTATTTTGCCTAATATAAAACAAAACGTCTAACTTCTTATTTATGCAAAGGTAAGTTAGACGTTGGAATTCTTTAATGGTGGAGATGAAGGGTCTCAAACCCTCAACCTCCTGAGTGCAAATCAGGTGCTCTATCAGTTGAGCTACATCCCCATTATTGGTGGAAGTAAATGGACTTGAACCATCGACCTCACCCTTATCAGGGGTGTGCTCTAACCAACTGAGCTATACTTCCAAGCATAATCCTAAGGGTATTTAACTAATTATTATAACAATTTTAATTTAACCAAAACACCCCTCGAATTTTAACAGTTTTTATAATCAAAACAGCTAATTTTATGGGGCTTGTTTTAAAAGAATTTAACAATAAAATAAGAACAGCATTAATTCTCGCTCCTTTTTTCACTTTCGCTCAGATAGTGATTGATTTAATCATTCCTTCTTTTTTAGCAAGTGCTATTTCAGTTGTGTTTTCAATTGATAAATTAAAACAGGATGAGTCAGGCGGAAAGACAATTTCAGTTGATTTTATTGGTGGGGCTAATATTAATTTTGCTAATGTAAGAGAAGCGCAAATAGTTTTAGCAACCACAGTTATTTTACTTGCATTATGTGGACTTTTTTTTGGTCTAATATCTATTTATTGTGCAAGTTATGTTTCAGCAAACACTTCTTATCTATTAAGAAAAAAGATCTTTGCAAAATTAATGCGAATCACAACCCCATCACATGACCATTATGGTTCTTCTACTTTATTAGTTAGATTAACAAATGATGTTTATTTGATGGAGGTAATTGCTTTTGATTTTTTAAGGCTAATTATTCGTGCTCCGCTTTTGTTTATAGGTGGATTAGTGTTTGCAGTAACTACAAATCAAGATATGTCAATATCTTTACTAATTACTTTTCCTTTGATTCTTTTGGTAATTGGTATTCTAAACCGTAAATCTATTCCTTTATTTAAAGAAAATCAAAAATCAGTTGATAAGATAAATGAAAGAGTAGAAGAAGATGTTTCAGGTTATAAAGTAATTCAATCTTTTAATCTTCATTCCTTTACAAATAACAAATTTAAGATTGCAAATGAAGGATGAAAAAAGAATAGTACAAGTTCTTTATTTATAAACTCACTTAACATTCCATTTACCTTTTTTTTAAGCAGTTTAACTATTATTATTGCTCTACTATTAGTTTTTCAATTAGATAGTAGTGTTTCAGTTGACCCATTACCGCAGGATGCAGCTATTAGACCTAATATCTTTGCTTTTTTCCAATATAACTTTTACATTGTTTTAGGGCTTATCTTAACCTCTTTAACAATGGTGAACTTTAATCGTTCTAGAGTTGCACTAGGCAGAATTAAAGATATTTTATCTCAGCCTGAAATAAAAACTATTACAAATAAAGATCAAAAAGAATTATTACCTACCTTGGAGTTTAGAAATATCTCTTTTGGTCTTGGCAATAAGAACAATAACAATTTTTTACAAAATCTTAGTTTTAAATTTGAAGCATATAAAACATATGGTATTGTTGGGCCTACAGGCTCTGGAAAATCTTTAATTGCTAACATTATCGGTGGTTTATATGAGCCTAATGAGGGTGAAATTCTCATTGGTGGGGAGAAAATTCAATCTATTGACAGTTTATATCTATCAGAAATGATAGGAATTGTTTTCCAACAAAACATCCTTTTTAAAGGAACAATTTCTTCTAATATAAAAATTGGTATTGAAACTAGAAGTGATTGAAAGAATCAATCTGATTTACAAAAGAATGAAGCGATGAAAAACGCAGCTAAAATAGCTTGCGCTGACACCTTTATTGAAAAGTTTTCTGATAGTTATGATCACAATGTTGAACAGTTAGGTAAAAACTTATCTGGTGGACAAAAACAAAGAGTTGCTATTGCAAGAACTTTAATTACAAAACCAAGAATTTTAGTTTTTGATGATAGTATGAGTGCTCTTGATGCTCTAACTGAAAAAAAAGTAAGAGAAAATATTGAAAATGATTTAAAGCTAACTACCAAAATCATTATTAGTCAAAACATTAATTCAATTAAACACGCAGATAAAATTTTGGTAATTGATAATGGCAGAATTGTTGGTTTTGATAGTGATCAAAAACTAATGAAAAATTGTTCACTTTATCAAAAGATGAAAGAATCTCAAAAAGATTTGGGAGGTGATTTTGATGCGGTTAACTAGTGTAAAACCAAGTTCTTGAAAGATTTTAAAGAGATTAATTACTTCAATGGAAGGAAGTTGAAGTTATAAATTACTTTATGTTTTTTTGTGTATAGTTCTTGGTATTTTATATGGAATTGCTAACCCTATCTTATTAGCACAAGGTCTTGGTTTTATTTTTCCTATTACTAATAGTAATGGTCGTGCTGTTGACTCAATATATTCATTAATTTACCCAACAAATTTAAATGTATTCATTAGGCTCACAATTGTGAGCGTAACTGTTTTTATAGCTTATGCATTAATCTTTGTATTTAATGTAGCGCAAAACTATGTAGGGATTAAACTTTACCAGCAAACATGTGCTACTTTGCGTTGAAAGGCATATTTAAAAATGCAGAGTATGTCAACCAGCTTTTTTGATACGCAAAATAATGGTGATCTTATGAGTAGGTTAACTAATGATATGTATAACATTGATAACCTATTCACTCAAGCTGGAGGACAAGCTATTCAAAGTTTGTTTAATATTTTAACAACCTCAGTATTAATATTTTTATTAAGTCCAGTTATTGCACCTATTTCACTTTCAATTTTAGCTACATTAATTACTTTTTCTTTTGCCTTTCTAAAGAAATCAAAAACTTCATATAGTCAAGTACAAAATAATTTGGGTGATATGTCTGGTTATATTGAAGAGGTTTTAACTAATCATAAGGTTGTTCATGTCTTGAAGTTGCAAGAGATAATGATTAAGGATTTTGATCAATACAACAAATCAATGATCAAATCAACTGTAAGAGGGAATACATATTCGATCTTTCTTTTTTCTTGGTTTGGTTTTATATCAAATATTACTTATCTGGTTTCTATATCAATTGCTACTGCTTTTAGTGTTAATTCTATTCCTTCATTTGGAATTAGTGTTATTAACTATTCATTCATGTTGTCTTACATTGCTTCTTTAAGGCAAATAACTTTAGCATTAGATAAAATCTTTACCCTTTGAAACTTAGTTCAATTAGGGGTTGTTAGTGCAGAAAGAGTATTTAAGGTATTAGATCTTAATGTAGAGAAAGATACTGCTACTATTGACAAATTACCTGATATTAAAGGCAATATAAGGTTTGAAAATGTAGCATTTGGTTACAATAAAGATAAACCTACTTTAACAGGAATTAACTTTAGTGTTAAACATGGAGATGTAGTTGCAATAGTAGGTCCTACAGGAGCTGGTAAATCAACTATTATTAATCTATTGATGAAATTCTATAAACCTTTTGAAGGAAAGATTTATATGGATAACTTTGAAATTAGTGATGTAACTAAAAAAGCATGAAGAGAAAAGATTTCTATAGTACTACAAGATTCATTCTTATTTAGCGGCACAATTAAAGAAAATATTCGTTTAGACAGACAGGATGCTACTGATGATGAGATTATCGCTGCATGTAAAACTGCTAATGCTCATGATTTCATCATGCGTTTACCAAAAGGATATGACACTTATATTTCCAATAAAGCAGATTATCTTTCTGTTGGTGAAAGGCAATTATTAACAATTGCCAGAGCAGTAATCCGTAATGCTCCAGTTTTGCTCTTAGATGAAGCAACTAGTTCAGTTGATGTCCATTCAGAAAAATTAATTCAAGAATCAATAGGAAGGTTAATGAAAAATAAAACTTCTTTTATAATTTCTCATCGTCTTTCAATTATTCGTGATGCAACATTAATAATGGTTATTAATGATGGTAAAGTACTTGAAATGGGTAATCATGATCAGCTGATGAAACAAAATGGATTTTATGCACGTTTAAAACAATCTTCGGTTCGTTAATAAATTCTAATGACTGTTGCTGAAATTAAAAAACTTGCATTAAATAATCAGGTATTTAATGAAGCAAAAGCGCTTTTAGAAAAAGGTAATGTTATTTTTCCAAAAAAATTCTTAAAGCGAAAGAAGATCATTATTGAAGTATTAGATGGTAAGGTTTTTAAGGTTCAAATTAATTTAAAAACCGCTGCTGCACACTTGGATTGTAGTTGCTCTAATGATAAGCAAAATTGCGTTCATATAATTGCAGCGCTTTTGAAATATAATGATCTAAAAAATCAAGATAACAAAGAATTTGACCTTAATAAAGCCGATAAATTAGAGTGCAAAGAAGTTGAAATTCTAATTGAAAATGTTAGCTTAGCAATTGTTAATGGCAGCTGAAAATTAAAGATAGGTTTTGTAATTAATATTGATAAAGTTCAAACTAATACAACTGCTTTACGTTTTTATTGTTGTGATAATAAAGATGTTTATTTTCTACATACTGAAGATGAACAACTTTTCAGGATTGCTTTAGATAAATTTAATAGTGTTGAAAGACAAACATTATTAATTTTTGATCAGCTAAATAAAACAAAGCAAATGCAATATGAAAATAACAGTTTGCTTTTTAATTTAGATCAATTCCTAAGCCTAGTTAAGGAAGTTAAAAAACCTTCATTATTCTTATTAAATGAGGATAAAACTGACAATATTCTTTTCTTAAGAAGTCAACATAAAATCAATGGATTAAGCCACGTTTGTGGTTTTTTAAATAATAAGGTTTTTGATTTTGTATCCTACAATGAAAAAACTAAGCAAATTGTTTTACGCTTAGCTTATCTTAATAAGTTCACTGATTTTAAATTTCCATACAACATTAACATCTATAAACTTGCTTTTGGAGAAACATTATTTTTCCATTTTTTAATCCATTTAAAAATGAATGGTTTTAAAAACATCTTTTTTCAAAGTGATGTTGCTATTGTTAAGGAAAGTGAATATCTACCTAAGATGTTTTTGACTATTGAATTTAATACACAAAAAAACAAGTTTATAACTGATGCTTTTTTTAAATATAAGAATAAAAATAGCAATACTTTAACAACTGTTTACCCCCATCGCTATTACTTAGCTCAAAAAACTAATACATCAAACTTCAACCGTTTACTTTTTTATGAACAAGCACTACAAAGGTTTTATGAAGAATTATTTCAAATAGATTATTTAAGAAGATTTGAAAACATTCCCATTAAAGATAAGAATCAAATTGCGCTTTTTAAAACTGTTTTTGATGATTACAAAACCATTGATTTAGCAGAATTGAAACTTACAAGTAATCTCTTAAATTACAAACAGTTACATTTTTCAATAAGTGATATTAAAGCTTTAAAAATTGAAGATAGACAACTAAAAATTGAATTTAAAGCTGGCGGGATAGATCTAAAGTTAATTAAAAGTGTTTTAAGTAACTACTATAAGGGTAATGCTATTTGTATTGGTGAAGATGGTTGATATGATTTGAACGATGAAAATGCTAAAGCACTAATTTCATTTTGGAGTCAAATTGACTTAAGAAATGCTACTTGTGATGCTAATAACAATTTGCTTCTTGCTAAATACCACTTGTTTGAAGTTGTAGATACTATTAGTAAATACACTGATGTAACTAATTTATTAGATGAAAAAACAGCCTTACAATTAAAAATAGCTAGTGAAAATCAATTTCATCTTTCGTTAGATAATAACCAAATTAATAACTTACGTAAATATCAAAAAGAAGGAGTTAAATGGATAAGGGCATTAGAAGATAATCAGTTTGGTGGAATTCTAGCAGATGAAATGGGGTTAGGTAAAACTGCTCAAGTGATCTTTGCAATGCTGGATAGTTATCAATCAACAAAATCACTTTTACCTAGTTTAATTATCGTTCCAGCATCCTTACTCTTAAATTGAAAAAGTGAGTTCCAAAAGTTTGCGCCTCATGTGAAAATAGTTACTGCTAATGGCAATTTTAAAGAACGCTCACAGATATATGAATCATTAAAAAATCAGATTTTGTTAATGAGTTTTAATGTCTTGAGAAGTGATATTAAATGAATATCTCAAAAAAAGTTCCATTATGTAGTAATTGATGAAGCACAAGGAATAAAAAATGAAAATTCTACTGTAACAAAAGCCGCTAAAAAAATTAAAGGTAATTTTTGCTTAGCACTTACCGGTACTCCTATTGAAAATCGTTTGCTTGATCTTTGATCTTGTTTTGATTTTGTTTTACCAAATTTTCTTGGTAATAAAAAACAGTTTTCAGATCAATTTGAAAAAGAAAAAAATGATGAAAGCTTTCAAAAATTAATGAAAAAAACAAGTCCTTTTATTTTAAGAAGGACTAAAAATAAAGTTTTAAAAGAACTACCTAAAAAAATTATTACTGATATCTATGTTGAACTTAGTGAAGAACATCAAAAACTGTATGATAAACAAAAAACAGATGGTTTGAAGGAGATTAAAGAAAGTGATGCTAAAAATGCTTTAAATATCCTTAGTTTAATCTTGAAATTAAGGCATATTTGTAGCTTAGTTAAAGATAATGATGTTAATGATTTTGAAGATAATTCCAAAGCTAATACAGCTTTAAACATTATTTATGAAGCACTTGAAAATAAACGCAAAGTTATTTTGTTTACTCAATTTTTAGATGTAATTGATTGTTTTAAGCAAACTTTAAAAAATCAAAAGATTGATCACCTGGTATTTGATGGTAGAAAAACTGTGAAGAATAGAAACACTATTATCCAGAAGTTCAATAGTGCTAAAGAACCTTCTGTGATGTTAGCTTCTTTAAAAGCTGGTGGAGTTGGTATTAACTTAACTGCTGCTGAAGTTGTTATCCATTTTGATGTGTGATGAAACAGTGCTGTTGAAAATCAAGCAACTGATAGAGCACATCGTATTGGTCAAAGTAAAACTGTACAAGTTTATAGAATTATTGCTAAAAATACTATTGAAGAGCGAGTTTGTCAAGTCCAGAATCAGAAACAAGAACTTGTTAAAAAACCTTGGTTGAGGATGTAAATTTCTTTAAATCTCTTTCACATGAAGAACTCTTAAAGCTTTTTGAATAAAGCAAGAATTATAATTAACACTCTAAGGATGCAAGTGATAAATGGCTGCTGGTAAAAGGGATTATTATGAAGTTCTAGGGATATCTAAAAACGCTAGTTCTCAAGACATAAAAAGAGCTTTTAGAAAGCTTGCAATGCAATATCACCCCGATCGTCATAAAGCAGAAAATGAAACTACTCAAAAACAAAATGAGGAAAAGTTTAAAGAGGTTAATGAAGCATACGAAGTTCTAAGTGATGAAGAAAAACGTAAGCTTTATGACCAGTTTGGTCATGAAGGGTTAAATGCTTCTGGTTTTCATGAAGCAGGGTTTAATCCTTTTGACATCTTTAATAGTGTTTTTGGTGAGGGATTTTCCTTTGGAATGGATGGTGATTCACCATTTGATTTCATTTTTAATCGTTCTAAAAAACGTCAACAACAAATTGTTGTTCCCTATAACCTTGATATTGCTTTAGTAATTGAAATTAACTTTTTTGAAATGACTAATGGTTGCAACAAAACCATCGAATATGAAAGAAAAGTTTCATGTCATAGTTGTAATGGTTTTGGCGCTGAAGGCGGGGAAAGTGGATTGGATCTTTGTAAGGATTGTAATGGCAATGGTTTTGTTATTAAAAACCAACGTTCTATCTTTGGAACCATTCAATCCCAAGTCTTGTGTTCAACTTGCAATGGACAAGGAAAACAAATTAAAGTTAAGTGCAAAACTTGTCGTTCTAACAAATACACTGTTACCAATCAAATTAAAGAGATTAATATTCCAGCAGGAATGTATAGTGGTGAAGCTTTAGTTGATGAAAGTGGTGGTAATGAATTTAAAGGTCACTATGGAAAATTAATCATTCAAGTGAATGTATTGGCAAGTAAGATTTTCAAACGTAGTGATAATAATGTTATTGCCAATGTTTTAGTAGATCCAATGGTTGCTATAGTTGGTGGGGTAATTGAACTACCTACTCTTGAAGGGATTAAAGAATTTAATATTAGACCAGGCACTAAGAGTGGCGAACAAATTGTTATTCCTAACGGTGGGATTAAATTCTCAAAGAGTTTTAAAAGAAAAGCTGGGGACTTAATCATTATTATTAGTTATGCACGTCCTTGTGAATACACTAACTTAGAATTGAAAAAATTACGTGAGTTTATCAAACCTAATCAAGAGGTTAGACAATACTTAAATACTTTAAAAAATGAATACAAAACTTAATGTAAAAGGTTATCTAAATGTTGGTGATAACCATCAACTTTATTACTGAACACAAGGAAATCCTAATGGTAAACCGGTTTTGTATATCCATGGCGGACCTGGTTCTGGTACTGATGAAGGATGTTTAAAGTATTTTGATCTTGAAACAACTTGGATTATTTTATTAGATCAAAGAGGTTGTGGTAAGAGCAAGACTAATGATATCTTTTATGAAAATAACACTGATAAATTAGTTAGTGATTTTGAAATTTTACGTCAAAAATTAAACATTAAAAGCTGAACACTCTTTGGTGGTAGTTGGGGTTCTGCACTTGCTTTAGTTTATGCAATTAAACACCCACAAGTAGTTGATAAGATCTTTTTAAGAGCACTTTTTTTAGCTAGAGGAAAAGACTGATCTGAAGCTTTAATGGGATTAGGAAAAATGTTTTATCCTTATGAACACCAACGCTTTATGGATAGTATTCCTAAAGCTTATCAGAACAGTTATGAACAAATTGTTAACTACTGTTATGATCAATTTCAAAATGGTGATGAATCAACCAAAGAAAAACTTGCTAAAGCTTGGGTGGATTGAGAATCAACATTACTTTCACCTATTAACAAAATTCATTCAACAGCAACAGACTTTAAATTAGTTGAAAAACTAGCTTTATTGGAATGTCACTATGCAGTTAATAAAAGTTTTTTGGATGAAAACTTCATTCTAGATAACATTAGTGTTCTTAAAAATAAAAGTATTTATTTGGCTCATGGTAGATTTGATTTGATCTGTCCTTTATATCAACCATTAGCATTAAAACAAGCATTCCCTGAATTACAACTTTATGTAACCAATAATGCTGGTCATAGTGGTAGTGATGCTAATAATTTAGCAACTATAAAACACCTTTTAAAAACTTACCTTTAATGAAGCGTTGTTATATTACAACCCCTATCTACTACGCATCAGGTAAGCCCCATATAGGTCATGCTTTTACCACTATTTTGGCGGATGTAATTAAGCGTTTTAAAATCCAAAACGGATATGAGGCTTTTTTGCTTGTTGGCAGTGATGAACATGGCAATAAAATAGAAAGTAAAGCTAAAAGCTTAAATTTAGATCCTAAAACATTTGTTGATATTAACGCTCAAGCTTTTAAGTTAATGTGAAAGACCCTTAATATTAGTTTTGATCACTTTATTAGAACAACTGATGAAATCCATAAACAACAAGTTCAAAAAACATTTCAAGATTTATATGACAAAAAACTAATTTATCAAAGTGAATGAAAAGGGGCATATTGTGTTGAGTGTGAACAAAATTACTTTACTTTTAACAAACAAACAATGTTATGTGAAATAGGTCATAATCTCAGTCTTGTTAAAGAACCTTGTTGATTTATTTCTTTTTCTTCTACTAAAAATTGAATTGAAACAATGATAGGCAAAAATCAACTTAACATTGTTCCTAAATCACGTGCTTCTGAATTAAAAAATAACTTTATAAACAATGGTTTAAACGATTTAGCATTAACAAGAAAAAATGTTACTTGAGGAATAAAAGTTCCTTTTGATCCAAATCAAACAATCTATGTTTGGTTTGATGCATTGTTTTCTTATATCACCAATTTAGGATTTAGAAATGGTGATCCTAGTTTTATAAAGTGGTGAAATAATGACAATAAAGAAAGAGAAGTTATCCATCTTATATCACGTGAAATCACCAGATTTCACTGCATCTATTGACCGATTTTTCTACACTTACTTGATATTAAGTTACCAACCCAATTTTTATCACATGGCTGGATAGTTGATGGTGAAGGGAGAAAGATGTCAAAATCTTTAAACAACGTTATCTCTCCAGAACAATTAATTGATCAATTTGGTGTTGATGGTACAAGATATTGTTTATTAAAAGAGATGCGTTTAGATAAAGATAATCGTTGTAGTATTAGCATCTTAAAAGAGATTTATAATGCTGATCTTGCCAATAGTTTTGGAAACCATGTTTCACGTACTTTTGGCATGATTAAAAAGTATCTAAACGGCAAATTAGAATACCAAATTATTACTGATAATGCACTTCAAAAAATAATGATTTTAATAGATGAATCAATCGTTCAATTTGATCATTACTTTAACAGTTATGAATTTTATAGAGCGATTAATCTACTTTTAAAAATTGTTTTTGAATTAAGTAAATTAATTGATGATTTCAAACCATGAGAATTGTTTAAAAATCAGGAATTCTCACTTTTAAAACAACTACTTTTTACTTGTGTTAGGTGTGTGCAGGTATGCTATGTGTTGTTAACACCTATCTTAGTAAATACTGCTTCAAAAGTTTTTCATTTATTTAATTTCGCTGATGATGCCTGTAGAAAAGACCAATTAAGAGATGCAACTTTATTAAAAAAAATTATTATCTCTAATTCAATGGAAGTTTTATTTAAAAGAGTAGATTAAGAAAAGTAGTATTAGAATTTTATTGATTTATGCAATTAGAGTACCTCAATCTTATTTCTCAAGCTAAAGTTATTGCAGAAAAACAATTTAAAGCTAACCCTTTTTCTTTTGAAACAATTTGAAAAGAAGTAGTTAAACATTTCAAGATTTCAAAACAAGATGAACCAAGCTTAATTGGTCGTTTTTATCAAGATTTTCTTGAGGATCCTAACTTTGTCTATTTAGGTGATAGAAAATGAAAACTTCGTGATTTTATGAAGTTTGATGAATGGAACAAGATATCACAATCTATGTTTGTTACAAAGGAGATTTTTGAAGAAGGTTATGAAGATCTTTCCAATAAAAAAGTAGAACCTGAGGAAGGAGTTGGTGATTTCATTATGGGAAATGATGGTGATGACAATGAAACTGGCAGTGAAATAGTACAAGGTTTAATTAATGATTCATTCAGTGAGGAAAATCAATAGTAGATATGCTTGTTAACTTTAAATTGATGCTTCAAAAAGCAAAGCTAGGTAAATATGCAATCCCTCACATTAACATCAATAACTATGAATGGGCCAAAGCTGTTTTAACAGCAGCAAATCAAGCTAATAGCCCAATTATTGTTTCAGTATCTGAAGGTGCTTTAAAGTACATGTCTGGTTATAGTGTTGTTATCCCGCTTGTTAAGGGTTTAATGGAATCACTAAGTATTAAAGTACCAGTGACATTACATTTAGATCATGGTAGTTATGATGCATGTGTCCAAGCATTACAGGCTGGATTTAGTTCAGTAATGTTTGATGGTTCACATTTACCATTTGAAGAAAATTTCAATAAATCTAAAAAGTTAATAGAGATAGCACAAAAAACAAATGCTTCTGTTGAACTTGAAGTTGGTACTATTGGTGGAGAAGAAGATGGTGTTATAGGACAAGGTGAGTTAGCTAATGTTGATGAATGTAAACAAATCGCTAGTTTAAAACCAGATGCTTTAGCAGCAGGAATTGGTAATATCCATGGTATCTATCCTAAGAATTGAAAAGGATTAAACTTTCCTTTGATTGAAACAATATCAAAAATTACTAACTTGCCCTTAGTTTTACATGGTGGCTCTGGAATCTTAGAAAATGATGTTAAAAAAGCAATTAGTTTAGGGATTTGCAAACTAAATATTAATACTGAGTGTCAATTAGCATTTGCACATGAAATTAGAAAATACATTGAATCAAATAAAGACTTGGATCTTAACAAAAAAGGTTATGATCCTAGAAAACTTTTAAAAGAACCTACTCAAGCAATTGTTGATACTTGCTTGGAAAAGATTGATTTGTGTGGTTCTAGAAATAAAGCATAGATGTTAAGTGCAGGGATAGTTGGTTTACCTAATGTTGGTAAGTCAACTTTATTTAGTGCTATTACTAATTTGCAAGTTGAAATAGCAAACTATCCTTTTGCAACTATAGAACCTAATACTGGCATTGTTAATGTTAGTGATGAGAGATTAGATAAATTAGCTAGCTTAATTAATCCTGAAAAGATAGTTTATACAACCTTTCGTTTTGTTGATATAGCAGGTCTTGTTAAAGGCGCCAGTCAAGGTCAAGGATTGGGTAATCAATTCTTAGCAAACATCCGTGAAGTGGATTTAATTTGTCATGTTGTAAGATGTTTTCAAGATAAAAAAATTGTTCATGTTAACAATACAATAGATCCTGTTTTTGATTTTGAAATTATTGTTAATGAACTAATCCAAGCTGATTTTGAATTAATAACAAACAGAATCAGCAAACTTAAAAGAAAAGCTGAATCAGGTGATAAAATCGCTAAAGAAGAGTTTGTATTACTTGAAATTGTTTTAAATGGATTAAAACAAGGTCAAATGCCCATTCAGACTCTAAGTGAAAGTGAATTGAAAACAATTAAATCACTTAATCTATTAACAGCTAAACCTATTCTAATAGTAGCCAATGTATCTGAGAATGACTTATTAAACCTTGATAATAATGAAGCTTTAAAAAAATTGAATGCTTTTCTTGATCAAAAAAAGATTCCCAAGGCAATCACAGTTTGTTCTTTAATTGAAAAAGAATTAAGCGGTTTGAAATTAGAACAACGTCAATACTTTTTGGATGAACTTGGCTTAAAAAATTATTCAGGTTTAAACCAAGTAATTCAAGCTGCATATCAAACTTTAAACCTTTGGTCTTTTTTTACTTTTGGTAAAAAAGAAGTTAGAGCATGAACATTCAAAAAGGGTTGAAATGCTCCTCAGTGTGCTGGGCAAATTCATTCTGATTTTGAAAAAGGATTTATTAAAGTTGAAGTAATTAGTTGAGATCAATTGTTTGCAATGAAATCTTTACAAGAAGCTAAAAAACAAGGTTTGATAAGATTGGAAGGCAAAAATTACTTAATAAAAGATGGTGATGTTTGTAACTTTAAATTTAACGTCACTTAAAAATTAATCTATTTTCAAAATGAGTTTTTTCAACATTTATTAATTAAAAATTAATGCTTTTTAAAGTTGAATCTTAATTCATTTTTCAATACTTTTTTAGGCTTCCTATTCTTTAATTAAATTAATACGCTTTTCTAAAAGTAAAAGTTAAGAAAAGTACTTTTATTCAAACAAAAACGTTTTTATAATTTTAGAGATAGTTGTGCCAAGTAAGTATTTATTCACCGTTATAATTCCAACATACAATTGTTGTCAATACATAAAAAAGGCATTAGATTCATTGCTTTTACAGAATGAATATTTTTTAAAAACACAAGTACTAATAGTTAACGATGGTTCACTAGACAATACTAAGGAAGTTGTTAGTGACTATTTAATAAAGTACTCCAATATTAGTTATTTTGAAAAAACTAATGGTAATTGGGGCAGTGTTATTAACTATGTTAAAAAAATAAATTAGCCTTAGGTCAATATATCACTGTTTTAGATAGCGATGATTATTTTTTAAAAGATAGTTTTAAAAAAGTGGCTCGTTTCTTTGGCCATGACATGATCATTGGCGCTTTTTATTGTTATATTAATGAAAATAAAACTCGTTTTTTAAAACCTTATTTCGGTAAGACTGGTGTTATTAAAGAACATACCAAATTAAGAACCCCCCATTCCCAACCTATTGCTAAATTTTACAGTAATAAACTTTTCTATGAACTACATGATCTTAAGGAAAAGTTATTCTTCCAGGATTGTTTAATGTATCACGATGCTATTAACAGAGTTGAAAGTGTTTTTTATTTGCGAGAGCCTTTAGCAGTTTGGTTTTCTACAAGACCTGGCAATTCTACAACAACTTCTTGAGAAAATCCAAATAAATTTAATGCTTGGTGTGAAATTCTCCAAAAAATGAATTTGTATGGAGCTGGGATAGTAATCTACATCTATACTATGCTACCTGGATTTCTAAAACAACTAAAGAAAAAAGAACTAATACTGAATTTGAACCATAAACCAGCTTACACTTGATTACCTAAACCTTTAGCGTTTATTTTTGGTGGTTTAATGGCATTTAAAACCAGAAAATACATTAAATATCCTAAGTAATTTATGGCAGAAATGATAGAAGCAAAAAATCTTCGTAATGGGCAAACCATCTTCGGTCCTAACAAAGAGATTTTATTAGTACTGGAAAATACATTTAACAAAACTGCAATGCGCCAGGGAATTGTTAAAACTAAAGTTAAGAACTTAAGAACTGGGGCTATTGTTTGGCTTGAATTTACTGGTGACAAATTAGAACAAGTAATTATTGATAAGAAAAAAATGAATTTCTTATACAAAGATGGTAATAACTTTGTTTTTATGGATCAAAAAGACTACAGTCAGATTGAGATTAATGAAAAAAAATTAGAGTGGGAAAAAAATTTCATTACTGAAGAAATTGAAGTTACTGTTATTACTTATCAAGATGAAATTCTAGGAGTTAATTTACCTGATTTAGTTCCTATTGAAGTTGAGTTTGCTGAAGATGCTATTCAGGGCAATACTGCTAACATGGCAAGAAAAAAAGCACGCCTTGTAACTGGTTATGAACTTGATGTACCCCAATTTATTAATACTGGTGATAAGATTGTAATTGCCACTATTGATGGCAATTACCGTGAAAGGTTTAACAAATAAATTAACTAGAACCCAAAGACGAATTGCTGTAGTGGAATTTATCTTTTCTCTCCTCTTTTTTTTACCTAAAGAAGCTGAAGTTATTCAAGCAGATTTTTTAGAATATGATACTAAAGAACGACAACTAAATGAATGACAAAAATTAATTGTTAAAGCATTTAGTGAAAATATCTTCTCTTTTCAAAAGAAAATTGAAGAACAACAATTGAAAAATCAATTAGAAATTCAAACTAAATACAATAAAATATCAGGAAAAAAGATTGATCTTTTAACTACTGCAGTTGTTTTATGTGCACTTAGTGAACAAAAGGCACATAATACTGATAAACCACTTTTAATTAGTGAGGCATTGTTGATTATGGATCGTTATTCACAAGGTGCTGAAAAAAAACAAACTCATGCTTTATTAGATAAGCTCTTGTAATGAAACGTAACTGAAGACAACACTATAATGTTTTTTTAGCTAATCTAGTTTTAGTATTTGGCTTTGCTTTAAATATTTTGGTTGCAAAACAATCTTTAAATAACACAACGCCCCAGTTCAGGTTTTTGTTTGTAACTCCTTTTCTTGGCGTTGTCATAGGTGCTGTTCTCTATTTTTTTGATGTTAAGTGGTTTTTAATTGACTATCCATACAAGAAATTTCACTTTCAAAAAAAATGAGCAATTGTTTATTTATCAGGGGTTATTGTATTTTTTTTAAATGTTTTAATTGGAGTAGTTTTACTTGTTGTAATGGTTAATTACATTACTAACCAAATTCTTAAAAGGGAATATGAAAGATTATTTACAAATTCATTACCTTATTTATGATCAACAACAGGAACTAGTATTGTTTTAAGTCTTATTAGTATAGGAATGAGTAAAACTGCACATTTTTTTATTGATATAGAGATTTTGAAAGCCAAGAAAGGAGAACCCACTGATCCTAATAAAACTGATAATAGAGCAGTTGTTATTAATCTTGATGAGAATAAAAAGAATGAAAAAGAACAGTCCCCTCCTTCTGCAGAAATGACTAGTCTTTAATTTCACTACCAAATAACCTTTTATGAACAAGCTCATTTAAAGTGTTATCTTTTAAAAAAGTGCACACAATAACTGTTGCAAATTCCAAAGCACAACCAGCACTTCTTGCTGTTATTAATTTATTTGCTACTGTTACTTTTGCTGTTGATCTGTTTTCAGACATAACTAAATTTGGGTTTGGAAAAGAAGAATAGATCTCATCTTTAGTGATAATACCATGTTTAAATAGAACATTCGGTGTGTCACAAATAGCAAAAAGATAAAGGTTATTTACTTTGAAATAATTAATTGTTTTAAGCAGTTTTTCATCTTTATCTAAGTGTTTTGTAGCTCCTATTCCACCGGGAATATAAACTGCATCAAATTCTGATAAGTTAATGGTGTTGACAATATTGTTAACTTCAACAACACCATTACTAGCAGTTATCTTTTTTAATGAATGGTGATAACATGTTGTTTGTATTGTTTTAATGAAACTAAATACAACCATTACATTAGTATATTCAACATCATTCATCTCTGGATAGACAATAACAAGTATCTTTTTCATTTTATTTAGTACCAAATAAACGGTCCCCAGCATCACCAAGACCAGGGATTATGTATCTATTGTCATTTAACTTTTCATCAATTGCTGCAAGAAATATATCAACATGAGGATGCATTTTTTCTACTTTATTAATTCCTTCAGGTGCTGCTACTATAGCAATAACACTAATTTTGATAGGTTTATCTTCTTTAATAGATTTAATAGCAGTTAACAATGTAGTTCCAGTAGCAAGCATAGGATCAAGAATAATAACATGTGAATCAGAGATGTTTTCAGGCATCTTTTTATAGTATGAAATTACACTGGTTGTTTGGGTTTGACGATAGATTCCTAAATGACCAACTCTGATTTTATCTGAATAGCGAACAATAGCATCAATCATTCCAAGTCCAGCACGCATAATAGGTACAAGAACAATGTCATTTTTTAATTTGTAGCCCTTTGTTTTAGCAAAGGGAGTTTCAACTTCAACTGTTGCTAGTGGTAGCTGTTTAGTTGCTTCAAAAAAGAGTAATGAAGTGATTTGATTCAAGGCCATGCGAAACTGGGAGGTTGTTGTATTTTTATCACGCAGTTTTGTCAATTCATTCAAGATTAAAGCATGTTGAACTTTTTTTATCACGTTAGTTATCTTTAAATAAGTTTAAACTAAAAATTAGTTGAAAAGTTTATGATCATCTTGATGTTCAAATTCATCAAACAGTTGTAATTGTTCCATCGTTTCAACGTGTTTTTTAGTAATTTTGGTTCTTTTTTTGAAATCATTTAAGGAAGCAAATGGTTTTTCATTTCTTGCTTCTACAATTGAAGAAGCTACCGCTTCTCCCATTCCTGGAATAGTGATGAAAGGCGCAATTAAAACACCATTATGTTCAACAAACATCCTTGCATTAGAGTTTTGAATAGAGATTTGTTGCAACTTTATATTGCGTGCCATCATTTCTAAATAAACTTCATAACTTATTAAAAGCTCCGCTTCTTTTGGTTTAATCTTTTTTATTCTGTAAAGCTTATTAAGTTCATCTAAACGGTTTTTAATAAATTCATACCCTTTTTCAAAACCATTAATATCATGTTCCTTTAGTTTGAAACTTAATAAACAAGCATAGTAACTCAAAGGGTGATAAAGCTTAAATCAAGCAATTCTTCAAGCCATTAAAACATAAGCTGCAGCATGTGCCTTAGGGAATAGATAATTAATTTTTAAACAAGCATTAATCCAATATTGTTCAACACCACAGTTTTGCATCAGGCTAACTTCTTTAGCATTTACTTTAATACCTTTTCTTACTTTTTCCATGATTTCAAAAGCATCTTTTGCTTGCATCCCTTTATTAATTAAATAAAGCATGATGTCATCTCTACAAGCAATTACATCTCTTAAAGTTAACCTATTGCTTTTAATTAGTTTTTGTGCATTATCTGCTCACACGTTTTTACCATGGCTTAATCCTGAAACTCTAATTAAGTCAGCAAAGTCTTTAGGTTTTGTCTGTTCTAAAATTTTTCTAACAAATTTAGTCCCAAATTCAGGGATACCAACCGCTCCTGTAACTTCATCAACAATGCCTGGTTTTAGGTTTAATGGTTTGTTTGAAGAAAACATCGAAATTAAGTTCTTATCAAAGTGAGGGATATTTTGGGGATTTATCTTAGTTAGATCAGCTAAGTGCTTTAACATTGTTGGATCATCTTGACCTAAGATATCAAGTTTTAATATGGCATCACCTAATGCATCATATTCAAAATGGGTGGTCTTTCATTCACTTTCAACATCATCTGCTGGAAAACCACAGGGAGTAAATTCATAAACAGAATGATCACTAGGAAAGATCATAATTCCCCCTGGATGTTGCCCTGTTGTTCTCTTAATTCCTATAAGCTTTTGTTTAAATCTTTCAATTTCAGCAGTAGTTGCAAGATCAACACGTTTAATGATTTCAAAATAGTTTCTAGCATAACCATAAGCAGTTTTTTCAGCAACTGTTGCAATTGTTCCTGCCCTGAAGGTGTTATTAACTCCAAATAATTTTCTGACATAATCATGGGCTTTAGCTTGATATTCGCTAGAAAAATTAAGATCGATATCAGGAATTTTATCACCAGAAAAACCCATAAAAGTAGCAAAAGGAATGTTATGACCATCACCTTTGAAACTTGCTTTTTCATGGCATTTAGGACAATCACGAATCATCAAATCATATCCATCATCAACACTGTCACTAACTTCAAAGTAATGACATTGTTCACAAAGATAATGAGCAGCTAATGGATTGATTTCTGATATACCAATTAAATTAGCAATTAAAGAAGAGCCTATAGAACCACGTGGACCTACAAAATAACCATCTTTAACAGATTGTTCTACTAATAGATGGGAAATTCAAAAGACAATTCCAAAACCATTACTAATGATTGCATTTAGTTCTTTTTCAATTCTTTCCTTAATAAGTTTAGGTAAATTTTTACCATACCTTTTTTCAGCTTGTTTTCAAGTTTTATCAATTAATTTTTGGTTAGAATCTTGCATCACTGGTGGATAAAGCTTGTTTTTAGTTGGTACTAATTCATTTAAGTCAAGTAGCTTCGCTATCTTATTAGTGTTTTCAACGACTAATTTATAGGCAATATCTTCTCCTAAAAAACTCATTCTTTTTAGCATCTCATCAGTGGTGTGAAGAAATACTTCAGGAACGGTTTGTTCTTTTTCTTTATTGTTAAAGTGTCTATGTCATTTCCCACCTAAGCCCTTAGCACAAACTATTGCCTTATAATATTCATTTTCCCAAGGATGGATAAAGTAAGCATCAGATGCAACAGCAACTAATTTCTTAAGTTTTGTTGCTGTTTTGATAACTAGTTTGATTGCATCATTAATAAGTTCTTTTTTCAGTCCTTCACGTAGTGTATAACCAAGGTAAGCATTGGGTTGTGAAATAAGCACAAAGTCAACTTTTTCAATTGCTTTTTCAAGTTCATTAATTGGTTTTGTTAATGCAGCTTTAAAGATGTCACCCTGAACAGGGTTTTCTGTTAATAAAAAAGATTTTCTAAATTTAGCTAAGCTGCTAGCTAAAACTAATGGTCTGTTAGCATTATGATCTGTTAATGCAATTGATAACATCTCATATAGGTTTTGAAAGCCCCGTTGGTTTTTTACATAAACGATTGCTGTGTTTGTAAATACCCTTTTCATCAGATCAATTTGACACTTTTTATTTAGGTTTTGATCTATCTCTGTTAAGGTATTAATACCCATTTCTTTTAACTGCTTTTTAAAGTAAAAGAAAACTTTTTTTAAAGCTTCTGTATCATATTCAGCACGGTGTAATCTTTCATCATCAAATTCAAGTTTTAGTTTAGAACAAATATTGCTTAAAGTATGGGATGAAAACAAGGGATTCAATGCTCATGATAAACATAAGGTATCAATCAGCGGGTTTGTTAATGGTTTGATGTTGTATTTTTCAAATTGAGTTTGCAAAAAGGGTAAATCAAAATTAATACCATTATGAGCTACCATAACACAATCATCTAGATAATTTCTTATCTTTTCTAAACCTTGCTGTTGATCAATACCGCCTTCAAGCATCTCATCAGTTATTTTGGTGATTTCAGTGATTGTTTTTGGGATAGGTTTGTCAATTTTTAAAAAGAATTGCTGATGATCTATCTCGCTGTTATTCTTAATTTTGCGTGCTGAAAACTCAATAACATCATCATACCTACCATGTAATCCAGTGGTTTCAATATCAAAAATAACAAAGGTGGCATCACTTAACTTAGTGTTATCTGGGTTGTGAACAATTTTGATGTGATCATCAGTTAAGTTGAATTCCAAACCATAGATAGCTTTTAAATCATATTTTTTAGCCACTTCATAAAACTTGGGATAAATATGGATATTATCTTTATCTGTAACAGTTATTGCTTTTCAACCTCTTTCTTTAGCAAACTGTGCATATTCTTCAATGTCATTGATACCATCAAAAGCAGTCATTTTAGTATGAAAAACTAACTCCACTCGCTTTTGTTTTGATAAATCTAACCTTTTATAGTTATTAGGTATTTCAACAGGATTTATCTCTCTAACAATTCCATATAAGATTTGAGTTTTAGGGTCACGTTCTACTTGGATATGGGCTTTTATCCAATTGCCAATAGTAATTCCTTCAATCTTTTTTTCATCAGTATAGGATCATTTCAAAATTAACGATCCACCTAATTGAAAATCAGTTACATAGATATTAAGTGTTTTTTTACCAGTTAAACTTTCATGGGTTTTTAATTCAAAGATTTGCCCAATAATCTTGACATCATCGATCTGTTGGTTAATCTTGTGGATAGGGATAAATTCTGTTGCAAATACCTTTGTTTCAAACTGCTTATCATATTGAGAAACAGTTACAGCTTTTACTTTTAACTTAGATTCATTTTTATCAATAGGATAAAGTGCAATGAAGTTAAAGTTTTTAAAACCAGCATTATTCATCCAAAAAATGAAATATTTACTTTTTTGAACTAATCATTCAGTTAATTCTTTGGTTTGACATTGTGCTTTTAGTTCGCCATTTTCATAACTAAGAAAATTGGGATTACTTAACTCTTGTTCAATGAGAATTTTGTATTTTTTATCTTTTGAAAAGAAACTGTGAAAATAGTCTTTAATAATAGCTATCGTTACAGAAGAAAAACTATTTTTTGATTTAAAGAATGGCTCATTCTCCTTAAAGGTAATGGTAAGTTTAAAAGATTCATTATCAGCTTTAAATCCCTCATTTAGTTCATTAAATCCTTCATATAAAGCATTTCAAATATCAATAGTTAAAGGGCGACTAATAGTTATTGCAAATAGAAAAACCTTCTCTTCAATATATTTATTAAAGTAAATTGATTCAATCCTTTCAATTAAGCTGTTAAGCTCATTGTGGTCAAGGATATTGTTATCTATCAAAAAATTGGAGAGTGCAAGAACCTTTTTATCTTTTTCATTCTCAAGATTAAATACCATAAGCGTTTATTAAAAAAATCAAAATAAAAGATCGAAATTTATCAATAATTATTTTTAGTTTTAGCCACTTTTTCGTAAAAAAAAAAAAAAAAAGCAGTTTTTGCTATTTTAGACTAAAAGTGGTTATATACCGAAATTTAAACCTTCTTTTGGTTAGTTCTTACTAACAGTTTTAAATGTTATACAAGTTTTTCAAAGTGTGAATTTAAAAAACTTTAATATAGTTGTTTATTCTGTATCTAATTAACCTTTAATCTTTCAATAATGAAAACAATTCATAAACTATTTTTAGGTCTTTGTTTACCCGCAACATTAGGTCCTTTACTTGGAATTGTTGTTACAAATACTGACCAAAGTATTAAGTTTACAAGCAAATCAAATTCGATTAATAAGAATAACCAAAATAAAGAGTTGGCTCTACTTAGAGATAATTTGATGAACGAAGCGAAAGTTGATGAACCACTTTCCTTTGAAAAACGGTTTGAAAACTTTAAAAATAAGTATAGTGATATACATAGCTTAAATAACAGTGTTTTTTCACTTCATGACGTTTATGACTTATTAGGTGGATTTAAACAATCATTGACAACATTTTTTGATGAAGTGATTGCCCAACAACAAAAGATCAAGGATGCAGATAAGATCTTTCCAAGTACTAAAGATAATCCACCTAAAGAAGAAAATCCTAATGTTTTAGATACACTAGCTAACTACTAAGGAGCAGGATTTTTCCCTAGTTTAGGTAAAAATGGTTTTAATTTACCTGAAGCAGTGTTCCAAAATTTCACTGATTTTAGGATTAATGACTACAAGATTAAAAATTTTAATGTTGATCTTGTTAGTGAAAATGACATTATTAAACATGATAAAGTTCGTTATGCTTTTGAAGTTAAGTTCAATATTGCTTTAGTTTTATCTATTAATAAGTCAAATGTTGATTTTGACTTTGATTTCATTTTAAAGACTGATAATTTCTCAGACATTGAAAACTTTAATGAAATTTTCAACAGAAAACCTGCTTTACAATTTCGTTTTTATACCAAGATCAATGTACATAAGTTAAGTTTCAATGGTAGTGATTCCACTTACATTGCCAATATCTTGTTACAAGATCAGTTCAACCTATTAGAAATTGATTTAAATAAATCTATTTATGCATTAGATCTTGAAAATGCTAAAGAACGCTTTGATAAGGAATTTGTTCAACCTCTTTATCAAAAACGACGTGAAGCAAAGCTTGCTTGAGAAGAAGAACAGAGACGCATTGCTGAAGAACAACGTAGACAAGAAGAGGAGAGAGCTAGAATCTTAAAAGAGTTAAAAGAAAAAGCTGAGAAAGATAAAAGAGTTAAAGAAGTACAAAACAACCTTCAAAAAGCACTTGGTAACTTAGATACTTTCTTTAACTTCTTTAGCAGTGGTCAAGATAGAGTTTTACTTGGTTTTGATCCAAATAAATACAATGTGCAAACTCGTGAAGGTTTGTTTAAAGCATTACAAATTTCCTATTCTAACTTCAAAACTTGAACATTCTATATCTCCTTGTTGGGGTGAAAAGAAGGTAGTGTTAAACTGTTGAAAAAACCTATCTGAAATGCCTTGAGAGATGATAAAGCATTTCAATATGCTTTTGGTTTAGGTCCAAATACTTCTGAACAACAACTTGGTAGAGTAACCCTACCTGGTTATGGTTATCAAGGAATTAGAATGAGTGATTGGTTGAGATGAGCATTGGGTTACTTACTAGTTTCACTTTAAGTCCACCTAAAAATGTTGAAGCTAATCTTATAGGTGATGCTAATGATAAAAAACACATTTGAATCTCACCTCATACTTTCAAATTAAACAGAGAGTATGGTGATGGTGAAAGATTCAAAGGTAAAGCATATCGTTTTAAACTATCAATAAGTTTTGAACTAGAAGGTCATTTAACTGCCCACTGATGAACAATTGCCTTTAGAGGTAGTATTCCTGGAAGCTGAAGTGGTAAGTTAAGAGTTACCCATGAGTTTGATGGTGATGTGCCTTACTATAGATTACATACAACTCCACCACAATATCGTTTAACTGATGATATGAAATTATTGTTTGTTCCGCACAGTATCCAAAGGGTAACTGCAGTTGGTAATGAAAGCATTAATGGTCTTCTCAGATCACAAAACCTTCATAACTTGGAACGTCAATCATATGAAGCGACTGCTCCTATTGATTTAATATCATATATGCTTTATGCAATTAGTGATAAAAAACCACCTCAAAAATAAGTTTTATATCATTAACGTTTAAATAAATATTATTGCTAAGATTACTTTAAACTTGTTAGCAAACTTTAATCATTTTTTAGCTAAATTAAACAACCAGTTAATTAGTAAACAGTTCCAGAATTAAAATCAAATATTAGTTAGTTTTTATTTTGTATTTTCAAAATTCTACTCAATTAGGATGATGGTTTCTTGCTGAATTGATAGGAACATTTATCCTAATTATCTTTGGTAATGGTGCAGTTGCTCAAGTTAATTTAAAGAAGATGGCTACAAGTGAAACAAAAGCCAAGTTTTTAACAGTTGCACTTACTTGAGGAATAGGTGTTTTATTTGGTGTTTTAACTGCTAATGCTATCTTTAAGGGTAGTGGTCATTTAAACCCTGCTATATCATTATTTTATGCAATTAATGGCAGTATCAAATCACCTACTGCATTAATATGACCTGGTTTTGTAATTGGGGTTTTAGCTCAATTCTTAGGTGCAATGATAGCTCAAACAACACTTAACTTTTTATTTTGAAAACAACTATCATCAACCGATCCACAAACAGTTCTAGCAATGCATTGTACAAGTCCTAGTGTATTTAACATTCCTAGGAATTTTCTAACTGAATTTATTGCAACTTTAATATTGATAGGTGGAGTTGTTGCTGCTAGTCACTTTCTTCATAACAACCCAAACTCTGTTCCTCCTGGATTTATGGGTCTTTGATTAGTTGCTGGGATTATTATTGCTTTTGGTGGCGCTACAGGCTCTGCAATTAATCCTGCAAGGGATTTGGGAACTAGAATTGTGTTTCAATTAACTCCAATTAAAAATAAGGATGCGAATTGAAAGTACAGCTGAATTCCAGTAATTGCTCCTTTATCTGCAGGATTAGTTTTATCAATAATTATTGGGTTTTCTCCTGCACCTGTTCTTTAAAGTTTTTGGTTTTAACAGTAACGTGCTTTTTAGTTAAAAATGAGTGGTGCAGTTTGCATCTAGCTTCATAAGCTTCTGCATCACCAATTAAGACAAGGTTATCATTAGTATTTTTATTTACTAGTCTTTGGGTTCGTTGTGCTAAACTACCACAAACATTGCAAATAGCATCAAGTTTATTAACTACATCAGCTATTGCTAAAAGTTGGGGAATACAACCAAAAGGCTCAGCTCTAAAATCAGTATCAAGCCCAGAAATAATCACATTTGTTCCTATCTCATTGAGAGTTGTAACAACTTCTATAATCTCATTTGAAAAGAATTGTGCTTCATCAATGGCAACAATTTGGTAGTTTTTATCAACTAAGTGATCATAGATCTCAAAAGGAGAATTAATAGTTATTGCTTGATCATATTCACCGTTGCGTGATTTGACAATATTAGTTTGTCTAGTATCAATTATTGGTTTGAAGATAACAACTGAAATTTTGGCTATCTTTCACCGTTTTATTTTGTGAAGTAATTTCTCTGTTTTTCCAGAAAACATTGGGCCACAAATAACTTCAGTTCAGCCCTTTTTGGCTTGAAAAGATGGTTGATATTTACCCATATAATTTCGAAATTATAATTAATGACACATGAACTTCTTGCAAAAACCAAGGGGAGTTAAAGATTGGTTTGGTGATGAATTAGTTTATTTTAATTGGATTGTTAAAAAAATAAGATCTTTAGCATTTAATTGGGGTTTTAGTGAAGTTAAAACTCCGTTGTTTGAAAATGCACAACTTTTTCAAAGATCTAATGCTAATGCTGATATTGTTCAAAAAGAACTATACCAGTTTTTTGATAAATCTCAAAGAGAATTAGCTTTAAGACCTGAAGCTACTACACCAATAGTAAGACTTGCTTGTGAAAACAAATTAATGCAAGAAGCAAATTTTCCCTTAAAGTTATTTTGCATTGGTTCAATGTATCGTTATGAACGTCCACAAAACAATAGGTTTCGTGAACATTGACAATTTAGTTGCGAAGTGTTTGGTTTTTCCAACCTGTTTATCTTTTTAGATACACTTTTGTTTGCTAACTCTTTGCTTGAAGCACTTGGAATTACTGGATATGTGCTTAAAATTAATAATCTTGCTAACTTTGAAACACTTAGTAAGTGAAATAAAGCCCTAAAAGATTACTTAACTCCATATAAATTAGAACTAACTGAGCTTTCTCAAAAAAGATTAGAAAAAAATCCTTTGAGAATTTTAGATGACAAGATAGATCAAAAAAAATCATTTGTTAAAAATGCTCCTAAAATTACTGATTTTTTAGATGCAAGTGCAAAACAAGATTCAGAATTGTTAAAAACACAACTAAAAAAACACAATATTAGTTTTGAATGAACAGACAATCTAGTTAGAGGATTGGATTACTATACTGGATTTGTGTTTGAATATGTAAAAAATCAAGACACAATTTTAGCAGGTGGAGTTTATGATAACTTAGTTGAAGAATTAAGTAGTAATCCAACTCCCGCATTAGGTTTTGCTTGTGGAATTGAACGGTTAATTAACTGTTTAGAAATTGATAAAAAAGCATTTATTTTGAATACTAAACCAAAGCAGATGTTAGTAATTTGCTTATTTGAAGAAGCGCTTGAAGAATTGGTTTGACTAGCTAAATTATGAAGGGAATATAACCAAGTAACTATTTATCCTAAGGTTATTAAAGTTGATAATGGGATTAGATTAGCAAATCGCTTGGGTTATACTTTCATTGGCATTGTTGGGAAAACTGATTTTGACAAAAAAGCTATTACAATCAAAAACTTAGTATCTAAACAACAGACCATTTACACTTGAAATGAACTTGGAGAACGAAAAGTGTTTTAACCAACGAATTTTAATTGGCTCAATTTCAACTGAACAACTCAATAAAACAATAGTTATTATTGGGTGAATTAAACGGATTAAAAAGTTAGGTGAAATTAACTTTATTATCGTTGGTGATAAATCAGGAACTATCCAAGTAACTTGCAAAGATAAAGAACAGATTCAACAACTTACAAGAGAAGACATAGTTATTGTTAAAGCCAAATTACAACGCTTAGATAGTGTTAGATTTGAACTGATAAATCCAACTATTAAACTTTTTTCAAAGTCAAAAACTCCTCCTTTAATTATTGAAGATGAAACTGATGCTTTAGAAGAAGTTAGGTTAAAATACCGTTACCTTGATCTGAGAAGACGTTTGATGCAAAAACGATTGTTATTGCGTCATCAATTTATATTAGCAATTCGTAACTGATTTAACCAGCAGGGTTTTATTGAAATAGAAACACCTACCTTATCCAAATCAACTCCTGAGGGAGCACAAGACTTTTTAGTTCCTGCAAGAATTAGAAAAGATTGTTTTTATGCTTTAGTTCAAAGTCCACAAATCTATAAGCAGCTCTTAATGATTGCAGGAGTTGAAAAATATTTTCAAATTGCAAGGGTCTATCGTGATGAAGATAGCAGAAAAGATCGTCAACCAGAACACACACAAATTGATTTCGAGATCTCTTTTTGTAACCAAAAAATGATTATGAATCTAGTTGAAAAACTCTTTTTTAGTGTTTTCTTAGATGTTTTTCAAATCAAAATAAAAAAGACTTTTCCTGTTTTTAAATTTTCAGAACTTTTTGAAAGATTTGGTAGCGATAAACCAGATTTACGTTATGGTTTTGAAATAAAAGATTTCACCTCGCTTTTTCAAGATCATCAGAATCAGTTCACTAAATTAATTGAAGCAAAAGGCATTATTGGTGGTATTGAACTTACTAATATTGAGTTAAGTACAGACAAAATTAAAGCATTAAGAAAAATTGCTAAGGACCATGATGTGAGTTTAGAAGTTCATAATAAAAATAATTCAACATTAAAAACTTCAATTAAATGTGATGAAAAAAACACTCTTCTGTTAGTAGCAAATAAATCTAAAAAGAAGGCATGAACTGCTTTAGGAGCAATTAGAAATGAGTTGAAATACCACTTGGATATTGTCAAACCTAACCAATACAGCTTTTGTTGAGTTGTTGATTTCCCTCTCTATGATTTTGATGAAAAAACAAATCAGTGAATATCAAATCACAACATCTTTTCAAAACCTAAACAAGAATGAATTGATAATTTTGAATCAAATAAAAACGAAGCATTAAGCGAACAGTTTGATCTTGTTTTAAACGGTTTTGAAATTGGTAGTGGTTCAATAAGAATTAATGATACAATTGTTCAAAAAAGACTAATGAATTCTTTGAACATTGACCCAAATAAGTTTGCTTTTCTTCTAGAAGCTTATGAATATGGTGCTCCTGTTCATGGTGGAATGGGACTAGGTATTGATCGTTTAATGATGATTCTTAATCAAACTGATAACATTAGAGAAGTAATCGCTTTTCCTAAGAATAATCATGGTATTGAAGTCCATACAAACGCTCCTGATAAAATTGACAAAGAGGAGGTTAAATGATGGATAAAAGAACTAGTGAAATAGAGTTTCACTTAAAGAATCTTTTAGCTTGTGATGCATATAAACTTTCACACCGTTTAATGTATCCACAAGATACACAAAACCTTTATAGTATGTTAACTGCAAGAGGTGTTTATGGTGATTTTAAGGAGTTTGTTTGAAACCATGATTTTGCTAAAAAGATCCTTTTGAATGTATTTAATGGTTTTGTAAACAGTGTAATTGAAGTTAAAAAAACAAATTGCTAGCTGCAGCATTGACAGATAAATTAGTTAGTGTTTTTAATGATCATGAATTGGCTAATGAATTCACACAACACATCTGTCATTTAGCTAGTTTCTTAGAAAAAAATAAAAAAATGCCGTTAGTTGCAAAGATTCATGAAAGTGATCAATCATTACCATTTAGAACTCCTTTAATAACTATAGAAGGAGTTGAAAATATTCCAAGCAACTTTGTATGGTTAGTTAATTACTTTGAAACTGTACTTTTAGAAAATATTTGGTTGTTTCAAACTGCTTCTACAGTTGCTAAAAGAATTAAATCTTTACTTGAAAAATATGCTAAAGAAACCGCAGATGAAACAAGTTTTATTAATTTTCAATGCCACGACTTTAGTATGCGGGGCATGAGTAGTTTGCAAAGTGCTTTGTATGTTGCTAGAGCACACTTGCAATACTTTACTGGAAGTGACACGATCTTAGGTGGGGATAATTCTCGTTCAATTTTAGCTTCTGAACATTCAGTGATGTGCGCAGATGGTAGTAAACATGAATTGAAAACTTTTCAACGTTTATTGGAAAAGTTTAAAGATAAAAAACTTTCTTTAGTGATTGATTCTTATGACATGTGAAATGTCCTTGATAACATTATTCCAAGGTTAAAAAATTTAATCTTAATGCGTGATGCTACGCTTTATTTGCGTGCTGATTCTGGTAATTATCAAACTCTTATTTGCAATCCTAATTACAAAAAGCAAGATAAAAGTACATGAGCAATGATCGATTACTTAGATCATCATTTCAGTTCAACTATAAATAAAAAAGGTTATAAGGTTTTAAACAACAAAATTGGAATTATTTATGGTGATGGAATCACCTATCAAAAGATAGAATGGATCTTAAATTGTTTAAAAAACCATGGTTATTGTTCTTCAAACATTATTTTTGGAGTTGGTAGTAGCACTTATCAAAATTTAAACCGTGATACTTTAGGTTTTGTATACAAATTAACTGCTATTAAAAAAAGTAATAGATGGATAGGCGTTAAAAAAACTCCCATAACTGATCTATCTAAAAGTTCAAAAGGCGGTAGATATAAAACAAAGAGATTAATTACAGTTTATTAATCAATTTCTTTCCATTTTAAAGCACGTTCAACTGCTTTATGTCAACTGTTAATTTTGGTTTTTCTTATGTTTGGGTCCATAGTGCTTTTGAACTTTTTATCAAGAGTAGTGAGTTTTTCAAGTTGATGAATGTCTTTTCAAAATCCACAAGCAAGTCCAGCTAAAAAACAAACACCAACTGCAGTGGTTTCTTTATTTTTAGGGATAGAAACAATTACATCTGCAATATCAGCTTGAAACTGCATTAAATAGTTTGATTTAACAATCCCCCCATCAGCTTTAATGCTAGTAATCTTATAACCTATATCACTTGCCATTGCATTTAATAAATCATTAGTTTGAAAAGCAATTGACTCTAAGCTAGCTTTTACTATGTGTTCTCTTTTAGTGCTTGCTTCAATTCCTAAGATAATACCCCTAGCACTAGCATCTCATCAAGGAGCTCCAAGTCCACTGAAAGCTGGTACAAAAACTAGGTTTTGTTCATTTTCTTTTGCAAGTTCTGCATAAAAATCACTTTCCTTTTCTGAATAGATAATTTTTAATGCATCCCTTAACCATTTTATAGCCGCACCCGCTACAAACACACTACCTTCCAATGCATATACAGGTGGATGATTTTCTAGTTGTCATGCTACTGTTGTGAGCAGATTGTGCTTTGAGAGTGTTGGTTTATCACCAATGTTCATGAGTGCAAAACATCCAGTACCATAGGTATTTTTTACCATTCCAGGTTCAGTACAGAGCTGACCAAACAAAGCTGCTTGCTGGTCTCCTAAAACTGCTCTAATTGGTACAATACCTTTAGCATTACTAGATCAGTGATTAGTTTCAATATCACCAAAGTAAGCATTGGAACTCAGAACTTTAGGTAAGATTGAAACTGGTACTTCAAATAAATCACATAACTCTTTGGATCACTCCATTTTGACAATGTCAAATAAAAGAGTTCTTGAAGCATTTGAAACATCTGTAACATGCATTTTTCCATTAGTTAGTTTTCAGATTAATCAGCTATCAATGGTGCCAAATAACAACTTTTTTTGCTCCATTAGTTTCTTTGCTAAAGGAACATTTTTTAAGATTCAAGCTATCTTAGTAGCACTAAAATAGGGGTTAATAGGTAATCCAGTTTTTTGTTTTACTTTGGTTTGGATTAACTTATCCTCATTGAATTTTTGACATAGTGCTGCAGTTCTTTGATCCTGTCAAACAATGGCATTATAAACAGGCAAACCATTCTCTTTATTTCATAAAACTATTGTTTCTCTTTGATTGGTAATACCAACTGCAATCACTTCATGAGATTTGATTTGTGCTTTATTTTTAGCACTTTGCATGGTAGCTAGTTGGGCTGATCAAATTTCTAGTGGATCTTGTTCAACTCAACCACTATTAGGAAAAAAAGTGTTAAATTCGTTTTGTGCTATTGCTATTTGGTTAAGATTGTGATCAAAAACAATTGATCGACAAGAACTAGTACCTTCATCTAAGGCAATGATGTATTGTTTTTTTAGATCCATGATAAGTTGTAATTAGGATTTCAATTTATCTTCTTTTTTAACTTTAAATTAAGGAGTTCAACTGCTCTCTTAGCAATAGCAGGACTTGATGTTAAGCCAGGGGATTTCATGCCACCAAGGATGATAAAGTTAGGATTGCTTTTTGCTGTTCTAATAACAAAGTCATTTGTTTCAATATCAATAGCTCTTGAACCTGCAAAACTATAAATACTATTTTCAAATTGCAATGAAGGAACCATTTTTTTACCAATAGTTTTAATTTGGTTAATTGAATCTAAATCTATGGAACGAGTTTTGTTTTTTTCAATTCCCTCAACTGCATTAGGTCCCACAAGAATATTACCATCTAACATCTCAGCAACAACAACTCCCTTACCATGGATAGTAGGTACCATAAAAATAATTGTGTTAATCTTAAGGTTGTTTTGATTTTTTAAAACTAGATATTGTCCTTTTCTTGTTGTTTGTTTAAAATTATCAACTTGTGTAGTTTCTGCTAGTCAATCTGCATAATGACCAGCTGCATCAATTAACTTTTTGGTTTTAAATTGTGGTGTTGTTTCATTGTTAATAAAAACTAAAAAATCATCATCAGAATCTATTTCAATTTTTGTAACTTTTTTATTTGAGTAGATGGCAACATTGTTTTGTAAACTAGCAAGGGCTAAACACTTGGTTGCAATTAAAGGATCAATTAGTCAACTACCTTCAACTTTTAAACTAGCTACTACATTTGGATTGATAAACGGTTCTTGCAATAAAGTTTGTTGTTGGTCTAATATTTGAATGTTTTCAACAGGAATTGAGTTTTTAATTCCCCTTTCTTTTAACAAATTTAATTGAAGTTTTTCTTCATTGTTGAAAGCAACAATTAAAGTAGCAATTTTTTTCCTTGGGAAGATTAGTTTTTTAAATCAATCCTCAATTCAGATTTTTCTCCCTAAGATGTTGTATTTGGCAGTTAGCTTATTAGGATTAGGATCAATTCCTGAATGGATTACACCACTGTTAGCCTGCGAAGTTTCACAACCCAAAAAAGCATTTTTTTCTAAAAGTGCAACTTTTAATTCATATTGGCTGAGTTCATATGCACAACTAGTACCGATGATACCCCCACCCACAATTAAAACGTCAATTGTTTGCATTAATTAATCTTCTAGTATTTAGTTAGTTATTTTGCAAAAAAGGATTAGTTTTATAGTTGTGATTTACTTTTTGGTATCTCTATAAAAAGTAGTATCAAACATTTTTTAATCTTTTGATTTCTTTTTACTAAAAATAATTAGCTAAAATACCGTCATATATGGGAAAGCTATTATTTGGTAAGTTAGTTTTTAAAAAGAGCTTGTTTTTACTGAGTGGTATGAGCAGTTTAGCAGTTTTTTTAACTGCATGCGGGGCTACCAAAATATTTGATTCCTCTGTACAGCTACTAGTATCAGATAACTTTTCCACACTTGCTGATAAATCATTTTCACAAATGTCCTATGAAGGAATCAGGAGCTTTTTTAAAAAAAGTAAGGGTGTTGATTTACCTGAAGCAGATAGTTCACAATTACAAGAAGGCAATGGCTTGTGAAAACGTCCTGGTTTTACATTAAGTGATAGAATTGCTACTTTTAATAACATCAAAAATGATGGCTCTGATGTTATTGTTGCAACAGGTTTTAACCAACAAGAATCACTTCAAGCAATTACTTCTGATGACATTAGGTTTCAAAGTGATAAAGAAAGCCTAGCTAAAACAGGTTTTATTTTTGTTGATGGAGCTATTGAAAAGGAGTTTAATAAAAGAAATGGTGTTCCTCAATTTAAGTCAACTCCTACCAATATCTCATCAGTTGCTTTTAGAAGTGATGATGGTTCTTTTTTAACTGGAGTTGCTACTGCAGTTTACTTAAATCTTAACCAAGAATATTTCCTTGATAAAAGTGGTTGGTCAACTAATAGTAGTAATAATAACGAACTAACTGTAAGTGGTTTTGTAGGTATTACTCTACCTTCAACGCTTTCTTTTCTAAATGGTTTTCGACTAGGTATTGCTTACTTTAATGAAGTGATTTATAAACATTTAAGCGATGCACAAGATTCATCTGCACAAGTGACCACTTCTAAACAAACTGTTTTAAAACAACTTCAAGTTGCAAATGGTGAAAAAAAGATTAAAAAGATTAAATGGATTTCACCAAAACAAGGAAGTGATGGAGAAACTATAAACATTCAAGATCACCAATCAGGTTCTTTTTCAGATACTGAACCTAGAGCAATAACAATAGCTAATAATTTAATTGATAAAGGAATTAATGCTATCATTCCTATTGCTGGACCACAAACGAATTTAGTGGTTACTCAAATTGCTAGAAGACAAGCCCATACTGCAGTTATTGGCGTTGATAGTGCACAGGAATTGCTAGATATTAATATTGATGCTCCAAATAAAGATAAGTTAAAAATGGGGAATAAAAAGATTATTCCCTTCTCTTCTATTAAGGCTTTGGATGTTGCTGTTGAAAGTATCTTATCAACATTAGAAAAAGGTTCCAGTCAAAATGGTTATCAGGGCTTTGGATATAACAACATAGGTACAGTAAAAAACAACTCTGTTGGGGTTAGTGAAGCAGGTTATGAATTTTTAATAGATCCTGTTTTTTGAAAAAATACTAGCTCAATGCAAGCTATGTCTTTATCAGCAAGTCTAAAAGCTAATGCAGCATCTTCATCAGATAATAAGAAAAAATTATCAGAAGTTGCTACTAAGAAAAATGAAAACGGTTCGACAAAAAATGGTAGTAATGGCATCATTGACAAATATGCCAAACTCTTAACAAAATCTAGTTCTTCAACTAGCATGAGAAACGGTAGTTCAGATAGCAATCAACAGAATTTTAAAACAACAGATAATGATGGTGATTGAACTATTGTTGGTGATGAATTAGGTAAATATAAGTCTAGTGAACTGCCTATTTTTACAGGTATTTCTTCATACCCAACTTTTCAAACTGAAGCACAAAATGTTTTAGATGGTGGAGCGAATGTTGCTTCAACACAAGGCTTTAAATGAAGCTTTAAACAAATTTAGAATTTTGATTACTTAAAGATATGAAAAAATTTCAAGCAGTTATTAAAGACCCAGTGGGAATTCACGCACGTCCTGCTTCTATCCTTACAAGTGAGGCTAGTAAGTTTAAATCTGAACTTAAACTGGTAGCTCCAAGTGGTGTTGAAGGTAATATTAAATCAATCATTAACTTAATGTCTTTAGGAATTAGACATAATGACAACATTACTATCAAAGCAGATGGAGCTGATGAAGAAGAAGCTTTAGCAGCTATTAAAGCTTGTCTTGAAAAAAATAAAGTTATCTAACTTAGCATATTTTAATCAATTAAATCTGCTTATTTTTTAATAGTAAAAACACAATTTAACCTTGTTTCTTATTTAACAAAATTGGTTTAAAAAAGGCTATCAATTTTGATGTAAAAAGTTAAGATATTTTTTATCAAAAATTAGCATAAAAAATTGTTATACTAATTAACGTTTTTTATTGAAAATTAAGTATTTAAATTGAACGAACATTCTTTAATTGAAATTGAAGGTTTGAACAAGACCTTTGATGATGGTTATGTTTCTATAAGAGACATTAGCCTAAATATTAAAAAAGGCGAATTTATTACTATTTTAGGCCCTTCTGGTTGTGGTAAAACTACCCTGTTGAGGTTATTAGCTGGATTTGAAGATCCTACTTATGGCAAGATCAAAGTTAATGGTATTGACATTAAAGACATGGCAATCCATAAGCGTCCTTTTGCGACAGTTTTTCAAGACTATGCTTTATTTTCCCATCTAACTGTTTATAAAAACATTGCTTATGGTCTGAAGGTAATGTGAACAAAGTTAGATGAAATTCCAAAACTTGTAAGTGATTATCAAAAGCAACTTGCTCTTAAGCATTTAAAGCTAGAAAGAAAAATAGAGCAGTTACAAAAAAACAATTCTAATGCTCAAAGAATAAAGAAATTAAAGGAAAAATTACAAAAACTTTTAGAAATTAACAAACAAAAAGTTATTGAGTTTGAAAATAAAGAAAAACTACGTAGAGAAGATATTTACAAGAATTTAGAGCAATTAACAAAAGAATGGGATCTACTTTCCCAAAAGAAACTAAAAGAAGTTGAACAGCAAAAACAAGCAATTGATAAAAGTTTTGAAAAAGTAGAGAATAAATACAAAAAAGATTCTTGGTTTTTTCAACACAGTGAAATGCGTTTAAAACAATATCAGAAGAAAAAAACTGAGTTGAAAGCTGATATTAAAGCAACAAAGAACAAAGAACAAATCCAAAAATTAACTAAAGAACTTCAAACCTTAAAACAAAAATACGCTAATAAAAAAGCAATTGACAAAGAGTATGACAAATTAGTTGTAGCTTACAATAAGAAAGACTATTGAACTTCTTATTGAGAAACATACACACTTCAACAAAAAGAAGCTTTTGAAAAACGTTATCTTTCAAGAAAACTAACTAAAGCTGAACAAAATAAAAAAGTTAGTGATGTTATTGAAATGGTTGGTTTAAAAGGTAAAGAAGATCGTTTGCCTGATGAATTATCAGGGGGAATGAAACAAAGAGTTGCTTTAGCACGTTCTTTAGTAGTAGAACCTGAAATTCTTTTATTAGATGAACCATTATCTGCACTTGATGCAAAGGTTAGAAAGAATTTACAAAAAGAATTACAACAAATTCATAAAAAAAGTGGATTGACTTTTATCTTAGTAACTCATGATCAAGAAGAGGCTTTAGTTTTATCAGATCGGATAGTTGTTATGAATGAGGGAAACATCTTACAAGTGGGTAATCCTGTTGACATTTATGACTCTCCTAAAACTGAATGAATTGCTAATTTCATTGGCCAAGCTAATATCTTTAAAGGTACTTATTTAGGAGAAAAAAAGATTCAGTTACAGAGTGGTGAAATCATTCAAACTGATGTTGATAATAACTATGTTGTAGGTAAGCAATATAAGATCTTAATTCGTCCTGAAGACTTTGATCTTGTTCCTGAAAATAAAGGTTTTTTAATGTTCGTGTTATTGATAAAAACTACAAAGGATTGCTTTGAAAGATAACAACACAATTAAAAGATAACACTATTGTTGATTTGGAGAGTGTTAATGAAGTTGATGTAAATAAGACCTTTGGTGTTTTATTTGATCCTATAGATGTTCATTTAATGGAAGTTTAACAAGATGCACATTAAGAAAAAATACTGACTTCTGTTCCCCTTCTTTTTATTAATGACAATCTTCTTTATTATTCCAATGACATGGATTATTGTTAGTGGATTACAAAGTGAAGATGGGGCTAGTATTAGTCAAAAATATGAACCACTTGTTAGTGGCTTAGGTTTTTTTAACAGTTTCTGAACCAGTTTGTGGATTTCCATAGTGACTGTAATTGTTGCATTGTTGTTTTCTTTTCCTTTTTGTTACTTTCTCTCCCAATCAAAAAACAAAATTTTTAAAGTGTTTGTTATTTCAATTGCAACAGTTCCTATTTGAAGTAGTTTTCTTATTAAGTTAATTGGATTGAAAACCCTACTTGATTTATTAATTGGACTTTCTTTAAACAGAGTTGGTGATAACAACTTAACTTTTGGTTCAGGATATACCTTACTGGGAACAATTTATCTGTTTACTCCTTTTATGTTTTTACCACTTTATAACAACTTCTGTGTTTTACCTAAAAACTTGTTGTTAGCTAGTCAAGATTTGGGTTATAACTGGATTTACAGCTTTGTGAAAGTAGTAATTCCTTTTTCTAAAACAGCAATGTTATCAGGAATTGCTTTAATTTTTTCCCTGCTTTAACTTCAGTTGCAATTGCTCAGTTTTTAGATAACTCTAACCAAGCTGAAACCCTTGGTAACTACATATTTACCTTGGGTAATAATGGTTATGATAGTGCAATTGAAAGAGGCAGAGCTGCTGGAGCAATTATTATTGCTGCTTTAATTACTTTTGCAATTTACTTTACTGTTGTTTTTTTGCCTAAAATTGTCCGTATTGTTCATAACAAATGAAAACAACATGAAAAAGCATTTTAAGAATTTAATTAAAAACAGTTATTTCTTTCTGTTAATAACTTTAATCTATTTACCACTTTTAATAGTTGTACTTGTTAGTTTAAACGGTTCTTCTTCAAGAGGAAATATAGTGCTTGATTTTGGTAATGTTTTAAATCCTAATCCTGATTCTAAATCTGCTTATTTAAGATTAGGTGAAACTGATTTTGCAACACCACTAATAAATTCAATCATTATAGGTGTGATCACTGTTTTAGTGTCTGTTCCTATTGCTGTTATCAGTGCGTTTGCGCTTTTAAGAACAAGGAATGCTTTAAAAAAGACAATCTTTGGAATTACTAATTTTTCTTTAGCAACTCCTGATATTATTACTGCTATCTCTTTAGTGTTGTTATTTGCTAACACTTGATTAAGTTTTAACCAGCAGTTAGGTTTTTTTACCATTATTACTTCCCATATCTCTTTTTCAGTGCCTTATGCATTGATTTTGATTTACCCTAAAATTCAAAAATTGAATCCTAATTTAATTCTTGCTTCTCAAGATTTAGGCTATTCGCCTTTAAAAACTTTTTTCCATATTACTCTACCTTATCTAATGCCAAGTATTTTTTCAGCAGTACTAGTAGTATTTGCAACTAGTTTTGATGATTATGTAATTACCTCTTTAGTACAAGGATCAGTAAAAACTATAGCAACTGAACTCTATTCATTTAGAAAAGGAATTAAAGCATGGGCAATCGCCTTTGGGTCTATTCTCATATTGATTAGTGTCTTAGGAGTCTGTTTAATAACCCTGCAAAAGTATTTAAGGGAAAAAAGAAAGGAAATAATCAAAATAAGACAATGAAAAAACAGTTAAAATATTGCTTTTTCTCACTTTTTGTTAGTCTCTCATCAATATTGAGTAGTTGTGGTTCAACAACATTTGTACTAGCTAACTTTGAATCTTATATTTCGCCCTTATTGCTAGAAAGAGTACAAGAAAAACATCCCTTAACTTTCTTGACTTATCCTAGTAATGAAAAACTAATTAATGGTTTTGCTAACAACACTTATTCAGTAGCAGTAGCATCTACTTATGCAGTTAGTGAATTGATAGAAAGGGATCTATTATCACCAATAGATTGAAGTCAGTTTAATCTGAAAAAAAGTAGTAGTTCAAGTGATAAAGTAAATAATGCCAGTGATGCAAAGGATTTGTTTATTGATTCAATTAAAGAGATCAGTCAACAAACCAAAGATAGTAAAAACAATGAATTACTGCATTGAGCAGTTCCTTATTTTCTTCAAAACTTAGTGTTTGTTTATCGTGGTGAAAAAATTAGTGAACTTGAACAGGAAAATGTTTCATGAACTGATGTAATTAAAGCAATTGTGAAACACAAAGATCGCTTTAATGATAATAGGTTAGTTTTCATTGATGATGCTAGAACGATCTTTTCACTTGCTAACATCGTTAATACTAACAACAATTCAACTGATGTTAATCCAAAGGAAAATGGAATTGGTTATTTCACTAATGTCTATGAAAGCTTTCAAAGACTTGGATTAACAAAATCTAATTTAGATAGTATCTTTGTTAATTCTGATTCCAATATTGTGATCAATGAATTGGCAAGTGGTAGAAGACAAGGAGGAATTGTTTACAATGGTGATGCAGTGTATGCTGCATTGGGCGGTGATTTACGTGATGAATTGAGTGAAGAACAGATTCCTGATGGGAACAACTTTCACATTGTGCAACCCAAAATTTCCCCAGTTGCTTTAGATCTTTTGGTTATCAATAAACAACAACCTAATTTTCAAAAAGAAGCACATGAGATCATTTTTGATCTTGCTTTGGATGGTGCTGATCAAACTAAAGAACAGTTAATTAAAACTGATGAAGAATTGGGTACTGATGATGAAGACTTTTACTTAAAAGGAGCGATGCAAAACTTTAGTTATGTGAACTATGTTTCACCATTAAAAGTAATATCTGATCCAAGTACTGGAATAGTCAATTCCAAAAGGAATAATGCTGAAATGAAAAGTAAACAAATGTCAACTGATCAAATGACTAGTGAAAAAGAATTTGATTATTACACTGAAACACTTAAAGCATTATTAGAGAAAAAAGATAGTGAAGAATTAAATGAAAATGAAAAAAAACTAGTTGAAACCATTAAGAAAGCTTACACTATTGAAAAAGATAGTTCAATTCAGTGAAACCAATTGGTTGAGAAACCAATTTCTCCCTTACAACGTAGTAATTTATCGCTATCTTGATTAGACTTTAAATTACACTGGTGATAATATGGAACAGCCGTTGTGTGTTTTAGGGATTGAAACAACCTGTGATGATACAGGTCTTAGTATTGTCATTGATCAAAAAATCAAGAGTAACATTGTTATCTCTTCTGCTAACTTACATGTAAAAACAGGAGGAGTTGTACCTGAAATTGCAGCACGATGCCACGAACAAAATCTCTTTAAAGCAATAAGAGATTTAAATTTTGAGATAAGAGATTTATCTCACATTGCTTATGCATGTAATCCTGGGTTAGCAGGATGTTTACATGTGGGAGCCACTTTTGCTAGAAGCTTAAGTTTCTTATTAGACAAACCATTGTTACCCATCAACCATCTTTATGCGCATATCTTTTCTTGTTTAATTGATCAAGATTTAAATAAGCTGCAATTACCAGCATTAGGCCTTGTAATTTCAGGTGGTCATACTGCTATTTATCTAGTTAAATCATTTTATGAACTTGAACTAATTGCTGAAACTAGTGATGATGCAATTGGTGAAGTTTATGACAAGATAGGCAGAGCAATGGGCTTTGATTATCCTGCTGGTAGTAAAATTGATAGTCTTTTTAATAAAGAATTAGTTAAACCTCACTATTTCTTTAAACCTTCTACTAAGTGAACTAAGTTTTCCTATTCTGGTTTAAAATCTCAGTGTTTAAACAAGATTAAACAAATAAGTGCTAATAAAACCCGAATTGATTGGAGTGAATTAGCATCCAATTTTCAAGCTACTATTATTGATCATTACATTGATCATGTTAAAAATGCAATTAAAAAATTTGCCCCTAAAATGTTGTTAGTAGGAGGTGGAGTTAGTGCCAATTCTTATCTATCTAACAGAATTAGTACATTAAATTTACCCTTTTTAATTGCTGATAGCAAATACACCAGTGATAATGGAGCAATGATTGGTTTTTATGCATCACTTTTAATTAATGGCGATAAGAATTAAAAGTACAAGAGTTGGTAGATTTGTTTCTGAATCAGTGGGATTAGGTCATCCTGATAAAATTTGTGATCAGATTGCAGATAGTATCTTAGACCAATGTTTACTACAGAGTAAAATTAGTCATGTAGCATGTGAAGTCTTTGCTTCTAAAAACCTTATTTTAATAGGTGGTGAGATTTCAACAAGTGGCTATGTTGATGTTGTTCAAACTGCTTGAAGAATTTTAAGAAATTTAGGTTACAACGAGACTGATTTCAGTTTTTTAAGCTGTATCAACAACCAATCACTAGAAATTAACCGAGCAGTTTTAAAAAATAATGAGATTAATGCAGGAGATCAAGGCATTACTGTTGGTTATGCAGTGAATGAAACAAAGCAACTAATGCCTTTAGGAGTTTTACTAGCACACTCGTTTTTAAAACAAGCAGAAAAACTAACAAAACAATTTGATTTTTTAAAAAATGATATGAAAAGTCAAGTGGTTTTAAACTACAGTTTAAACCAAGTTGAATGTGAAGAAGTTTTACTATCAATTCAACACACTAATGCTATTAGTTTAACAGAATTGAGAAAAGTGATTGAAAATAATGTAATTCTACCTGTTTTAAACCAATATGGTTTTCAAGATAAAAAGCCAACTTGTTTAGTGAATCCTGGTGGTTCTTTTGTTTTAGGTGGACCTATGGCAGATACTGGATTAACTGGTAGAAAAATCATTGTTGACACCTATGGTCCATATGCTCACCATGGTGGTGGTAGCTTTAGCGGAAAAGATCCTAGTAAGGTGGATAGAACAGGTGCTTATTTTGCACGTTTTATCGCAAAACATATTGTAAGTTTAGGCTGGGCCAGTGAGTGTGAAGTCAGTATTAGCTGAGTCTTTTCAAAACCCAATCCACAATCTATTACTGTTAAGTGTTTTAACACTAACATACAGTATGATGAAGTGTTAATTAATAGAGTTGTAAATAACTATTTCAACTGATCGATTACTAAAATTATTGACAAGCTAAAATTACTTGATTTTGTTAAGTATTCTGATTATGCAGTTTATGGACATTTTGGTAATGATCTTTCACCATGAGAACAGCTCACTGAATTGGATAAATTAGAATGCTTAATCAAAAATTTCCATTAGATCCTAATAAAAAAAAGGAACAACAAGGCGTAGTAAAACCAAATCTACCGGTTGTTAAAGAAAAGAAAAACAGCCATTTAAAAAACCAAATTGGAGTGAATTTAAGCTGTTCAACTTTTTTAAAAAGCACACTTATTTTTGCTTGGTTGTATTAGCTTTTCTTATCTTGATTATTTTACTTAGCAGCTTATTTGCTATTCCACTTAGCCAAATACCTTCAAGTACTGTTTAAAAGAATTGTCCAAATCCACCAAACGGGTTACTACCTGTTTTTAATAGTTTACCCATTTCTGTTGCTTTTAGTTGCATCTTATTTCATTCCTTCATCAGTTTGTTTAACTCATCCATTTTTCTCCCTGATCCTTTTATGATGCGCTGTTTTCTATTAGGATCACGATTAATTAATTTGGGATGTCTCCTTTCTTCTCTAGTCATAGAGTTAATTAAAACCTTTCATAGTTCAATTTTGTTTTCAATTAATTCAGCATTTTCTTCTGATACAGAAAAGTTAGCAGGCAACATTTTTATCAGTGAACTGACACTTCCCATTTTGTGCATTTGTTGCATGTAGATCAAAAGATCTTCTAAATCCATTTTTCCCAAAAACATCTTGCTGATGGTTTTAGTTAAATCTTTTTTATCAAAAACTTGTTCAGCTTTTTCAACTAAACTCATTACATCACCCAAACCTAAGATCCGATTGGCTATCCTTTCAGGATGAAATTGTTCCAATCCATCTAATTTTTCAGAAACACCAATTAATTTAATGGGTACTTGTAAAAGTGAAGCTAATGAAAGTGCAGCTCCTGCTCTAGCATCACTGTCTAATTTACTGATAATAAATCCAGTTAGTTTTAAACGTTTGTGGAACGTTTGAGCAACATTGATAATTTCCTGACCGCTTAATCCATCTACTACCATGATAATTTCATCAGGATTTAATTCATTTTTAACACTTACCAATTCATCCATTAATGTTTCATTTGTTTGTAATCTACCAGCGGTATCACAAATGATTGCTTGACATTTTGCAGTTTTAAAAGCACTGAGTGCTGCTTTTGTTGTTTTAGCAACTGGTTGAGTGCCTTGTGCAAAAAATACGCTGTTAGTTTGTTGTGAAAGCGTTTCAAGTTGTTCAATGGCAGCGGGTCTGTAGATGTCCAAGCCTACTAACATTGTTTTTTGCTTGTATTTCTTTTCAAGTCAATAAGCTAGTTTGCCACAAGTTGTTGTTTTACCTGATCCTTGTAAACCAACCATCATTATTTTTAAAGGTCTTTTTTCATTTAGTTCTTGGTTGGGTTGGCTTAAGATATTAATTAGTTCTGTTTTGATTGTTTTTAATAGAGACTTTTGCAAATCTTGACCAGGTTCAATGGTTTGTCCTACTGTTTTATCTCTAATTGCTTTGATGAAATTTTTAACAACAAGCAGGTTAACATCAGCATCAAGCAATGCAATTCTAATCTCTTTTAGAACTAACTCTACATCTTTCTCAGTGATCGTTTGAGCGTTAATTTTTTTTTGCATCGTGCGCATAACGATGCTTGATAACATTGCTTTGAACATGATTTTTAATTATTTATTATTAAATAATGTTTTAATAAAACAATATTGCAATATGACCCCACATATAAGTGCTAAGAAAGATGACATTAGCAAAGTTGTTTTAATGCCAGGTGATCCATTGAGAGCTAAATGGATAGCTGAGCAATTCTTAGATCAAGCTAAATTAGTCAATGAAGTGAGGGGAATGTTTGCTTATACTGGGCAGTATAAATCTAAAACAGTTACAGTAATGGGCCATGGAATGGGGATCCCTTCTATTGGAATTTATTCATATGAGTTGATGAATTTTTATGAGGTTGAAACTATCATTAGAATCGGAAGTTGTGGTGCTTTAGCACCGCAATTAAAATTAAAAGATCTTGTTATTGCTTCAAAAGCATGAAGTGAGTCTATTTATGCTAAAGACATGGGTGTTGAAATTCCAGAAGATAAGATCTTATTTGCAACAAGTTCTTTAGTGGAATTAGCAAAAGAAACTGCGATTAAGAACAAGCTTGATTTTCATGAAGGATTAGTATTTTGTGAGGATGCTTTTTATCAAACTAGAAAAGATGTAATTAGTCTTGCTAAAGAAAAAAATAGTTTAGCAGTTGAAATGGAAGCACATGCACTTTATGCTAATGCAATCCTGTTGAAGAAAAAAGCACTTACACTCTTAACAGTATCTGATTCTCTAGTAACTCATGAAGCACTTAGTTCTGAATTAAGACAAAAGTCATTTAAGCAAATGGCTTTATTAGCACTTGAAATGACTCAAAAACTAATCTAATCTATGAAATTAGAATACAACCGGATTATTGATAGCACCTTAGTCAAAGCTGATACGCTTCCCCATGAAATAGATACTTTATGTGCTGATGCTCATAAATACCAGTTTTTTGCAGTATGTGTTAATCCTAGTTATGTTAGTTGTGCTAAAAATATCTTGAAAAATACTGCAGTTCAACTCTGTTGTGTTGTTGGTTTTCCCTTAGGACAAACAACCCAAAAACAGAAGGTATATGAAGCTAAGATTGCTATTAAAGAGGGAGCGGATGAAATTGATATGGTAATGAATATTGCTGAGTTTAAAAAACGTTGTGCTTGTGTTATTACTGAAATTAGAGCTGTTAAAAAAGTGTGTGGCAAGCGTAAATTAAAAGTAATTATTGAAACTGCACTTTTAACAAATGATGAAATCAAAGATGCAGTTAATGTTTGCATTGATGGCAATGCAGATTATGTTAAAACTTCCACTGGTTTTTCTTTCCGTGGTGCATCTTTAGAAGATGTTCAGATTATGAATAATGCTGCAGCAAATTTAATTAAAATCAAAGCTTCAGGTGGGATTAAAACAGCAAAGCAATTTATAGATTTATTTCAAGCTGGAGCTAGTAGAATTGGAACTTCAAATGCGGTCCAAATAATGCAAGAATTAAACAAAATGAACCATGAATATCATTAATTTAATTAATAAAAAACAACGCGGAAAAGCTTTAAATTTAGCTGAAATCAATTGGTTTGTTAATGCTGTTTTAAACAAAACCATTGCTGATTATCAAATTACTGCATTTTTGATGGCTATTTGGTTTAAAGGGATGAACCCAAATGAACTTTTTTTATTAACAAAAGCAATGGTGGATACTGGTGAAATTATTAAGTTTAATCACCATGGCAAGATTAGTGTTGATAAACATTCAACTGGTGGTATTGGTGATAAGGTTTCTTTAGCATTGGTTCCTATCTTAACTAGTTTAGGATTTAGTGTTGCTAAATTATCAGGAAGAGGCCTTGGTTATACTGGTGGAACAATTGATAAATTAGAAGCAGTTGGAGTTAAAACAGAATTAACTGACCAACAAGCACAAGCATGTTTAGATAAAAATGATTGTTTTATCATCGGACAAAGTAAGGACATTGCACCAGTTGATAAAGTACTTTATGGTTTAAGAGATATTACTGGAACAGTTGATAGTTTGCCTTTAATTGCATCTAGTATTATGTCTAAAAAGCTAGCAGTTATGAACGAGTATATTTTCATTGATCTTAAATATGGAAAAGGTGCCTTTTGTAAAACTAAGAAAATTGCTAACGAACTTGCAAAACTGATGCAAAGTATTGCTAAAAGTTTTAAAAGAAAGCTGTCTGTTAAATTAAGTGATATGAATCAAGTACTTGGTAAAGCTGTTGGTAACGTAATTGAAGTTAATGAAGCTGTTAACTTTCTAAAACAAGATTTAGATCAAGTAGGACAAGATTTTATTGATTTAATGCAAGCAATTGTTATTAACATTCTACTTGAAACAAAACAAGCAAAAACCAAACAAAAGGCTATTGAACTTTATCAGGATGTTTTAACTAGTAAAAAAGCATGAAATCGCTTTTTATCTTTTATTGAATCTCAAGGAGGAAATGTTGAATTATTTACTCAAAAAGAAGGTTTTTTTAAACCTAAGTATAAGGCATCTATAAAAGCTGAAAAAAGTGGTATACTACATTTTACTGATCCAATTGATTTAGCTAAAATTGGGATTAATCTAGGGGCAGGTAGGATTAAGAAAACAGATCAAATTGATCCAATGGCAGGGTTATTTTTAGTGAAAAAAGATAATGAGTCTGTGGCAGTTGGAGATACTGTATTAAACCTGTATAGTTCTAATCCTATTAGCAATGAAGATATCTCTGCTGCTCAAAAAACAATAATTATTAATAAATAAAAATTCCTATGAAGGTGAATTTAGAGTGGATAATTAAACAGTTACAAATGATAGTTAAAAGAGCATATACTCCCTTTTCTAACTTTAAAGTTGCATGTATGATTATTGCTAACAACCAAACTTTTTTTGGAGTTAACATTGAAAATTCTTCCTTTCCAGTAACTTTGTGTGCTGAAAGAAGCGCCATTGCTAGCATGGTTACAAGTGGTCATAGGAAAATTGATTATGTTTTTGTTTACTTCAATACTAAAAATAAGAGTAACTCACCCTGTGGAATGTGCAGACAAAACTTACTGGAATTTTCCCATCAAAAAACAAAGCTTTTTTGTATTGATAATGATAGTAGTTATAAACAATTTTCCATTGATGAATTATTAATGAATGGTTTTAAAAAGAGCTAATGGATAAACTTAGATTAGAAGTTGAAAGATGGTTAAATCATCCTAATGTTAATTGGGAGTTAAAACAACAAATTAAGGAGTTGAATGAATCAGAAATTCAAGAACTTTTTAGTTTGGAAAAACCTTTATTTGGCACTGCAGGTGTAAGAAACAAAATGGCACCAGGTTATCATGGTATGAATGTTTTTTCTTATGCCTATTTGACCCAAGGTTATGTTAAGTACATTGAATCAATCAATGAACCAAAGCGTCAACTACGGTTTTTAGTAGCACGTGATACAAGAAAAAATGGTGGTTTATTTTTAGAAACGGTTTGTGATGTAATTACATCTATGGGTCATTTGGCTTATGTGTTTGATGATAACCAGCCAGTTTCAACACCTCTAGTGTCCCATGTCATTTTTAAATATGGTTTTAGTGGAGGTATTAATATCACAGCTAGCCATAACCCTAAAGATGATAATGGTTTTAAGGTTTATGATCATACTGGTGCACAGCTTTTAGACACACAAACAAACCAATTGTTAAGTGATTTACCTTGTGTTACATCTATGCTAGATTTGGAATTACAACCAAATCCAAAGTTTGTCCATACTCTTGACAATGAAAAGGTTTATAAAAACTATTTCAGAGAGTTGAAAAAGGTGTTGGTTATTAACAACAACAATTTCAAAGACATTAAGGTAGTTTTTAGTGGGCTTAATGGGACTTCAGTTTGCTTAATGCAACGCTTTTTAAAGTACCTTGGTTATAGCAATATTATCAGTGTTGAGGAACAAAATTGGTTTGATGAGAATTTTGAAAATGCTCCTAACTTAAATCCAGAGTATAAAGATACATGGATATTAGCACAAAAATATGCTAAGAAAAATAATGCTAAGTTAATTATTATGGCAGACCCTGATGCTGATAGATTTGCAATTGCAGAGTTAAATAATAATCAATGACATTATTTTTCAGGTAATGAAACAGGAGCAATTACTGCTTACTATAAACTTAATCATAAGGTTTTTAAATCACCTTACATTGTCTCAACTTTTGTCTCAACTTATTTGGTAAATAAGATTGCTAAAAGATATGGCGCTTTTGTGCATAGAACCAATGTTGGTTTTAAGTACATTGGTCAAGCAATTAATGAGTTATCACAAACAAACGAATTAGTTGTTGGTTTTGAAGAGGCAATTGGTTTAATAACTAGTGATAAATTAAACCGCGAGAAAGATGCTTATCAAGCTGCTGCATTATTGCTTGAGATTGCTAGACATTGCAAAGAACAAAACATCACGCTTTTAGATTTTTATAAAAGAATTCTTTCTGAGTTTGGTGAATATTTCAATTTAACAATATCTCATCCCTTTAAAGCTACTGCTACTGATTGAAAAGAAGAGATTAAAGCTTTATTTAATCAACTTATAAATGCTAATTTAACTGAAGTGGCTGGTTTTAAAGTAGTTAAAGTCCATCTTGATAAACAAACAAATATCTTAGAGTTTGGTTTTGAAAATGGCTGGGTTAAATTCCGCTTTTCAGGTACTGAACCTAAATTGAAATTTTACTTTGACCTAACTAATGGCACTAGAGAGGCTCTAGAAAAGCAAGCTAAGAAAATTTATAAATTCTTTGTAAATTTACTCAAACTCAACAAAGCTTAAATTTTCTAAAGGTAAGTTAATTAATTGGGTTTTAGATGTTAATTCTATCTCTACAACTGCTTTGTTAACTGTACCTTGATAAAGTGCTTTTATCTGTCCTTGAACATGAATAGTATCAGTTAAATGTACCTCTACTAAATTACCAACACTAAATTCAAAGAGTTGTTCAACTTTATTGTCGGTATCAAGAATTTCATATTTGCCCAAAAAAGGAACTAATACTGATGGATCAAGTTTGGTTTGCTCACTTTGAATAATCGTTGCTTTTTCTTTCCATTCATCTTGTTCTTGACTATTTTGTAGTTTTTTAAAAGCTTCTTTTAGTTTTTCAGCATCAACAATTTCTGAATCTTCATCCTCTTTTGGTTTTTGAATGGCTTGTTTTTGTTTTAAGATATATTGAAATTTTTCATCAAATTTATCACTGTCCATTTCAATAATGGCAGTATTTGTTAGCATAATTCGTTTGCTAGGATTGATTGATATCCCTTTAAGCATTTGTTCTAAATTATTAGCATCTTTTTCATCTAAAGGGATAGGTAAAGCACCCCTGCCAGAAGAACCTACTATGCCAGTGATCCCAGGGAAGTTACGAACAATTCTTCAAGCATCTTCACTATAAATCATCTTTATGTAGATGTATCTACCAAGGAGATTTTTTTCACTAATTTTTACCCTAAGATAACGGTAATCATCAAGAACAAACCATTTGTTAAAAGTAGTGTTCTTTAAGGAACGAGGAAGTTTTCCTGATTTTAATGAATAAACTTCTTCATGAACTTCCCTTTCTTTTAGAACTTTAACATCAACAATCTCATGATTAAATCCTAAAGCTTGAATTTTAGCTTTTAGATTTTTTACAACAGCTTCATCTTTAATACTAACAGGAGCTACATACCACTTTGGTGTTAATTCACTAGCTTGCATAAATTAATTTTTAAATATTCCCAACGTAATTAACAATTGATTGATACCAAAAAAGATTCCTACCATCAGTCCACTAACTAGCAAAATTAAAAGAAAAATTATGATAAGTTGTCTACCTTTTACTCAAACAATTTTGTTGATCTCTTTATCAACTCCAAATCATAAATTAACAATTCGTTTTTTAAAAGGAAGCTTGGGTTTCTTTTCCTTTGCTAAACTTTCATGCTTTTCTTTTTCTTTAGTTCTTTCCTTATCTTTACTTTTCTTGGCTTCTGTTCTAAGTTTGAGCTGTTTATGTAACTCATGAATTGAAGCATCATCATAAGCAGTTAGCTTTTCCTTCTTTTTAAAGCTAAAAGGCAGTTTTTTTTCCATGGCTTTTTAAAAAGAATCTAGATGCTTAGTTTTTTGATTGCACTGCTTGCAGTACTTGTTGATGATAAGTCGCTGCAAAGGTTGTTTTGTTCACCGTTTTACATAGTTACGACTTAAACAGTCTTGACAGACAAAAATAATCTTTTTGCGCAACTTCAGTAGCAAATTATAAATTATCTGCTTGTTTTACTAATTTAATCTTTTGATAGCAATAGAGCTGTAAAAGGCCAAAGGGTAAAAAACAAGATCAGTGTAGAATTTTTTTGAATTTAGGTTCAATATTTTCACATGCAGTATTAATTGTTTTTCAAATCATTATGCTGATAACTAACTGGATGATCATAAAACAGATAGCAAAAACTCCTAAAACAATTAAAACTGCTCGTAAATTAAAAAAAATAGGATCACTTCTAATTGCACTTAAACTAGCTTTTCTAATTAAATCAAAGATGATACCAATCCCTACTAAAGAGATAATACAAACTAAAAAAAAGATACTAATTAACAAAAGAATCTCATTAAGGTTATGGAGTTTTTTAAATACTTTCATGGAAAAGTGTATAGAGCATACTTGATTTTAAATGCATTTTTTTAGCTAAATAATTGCATGCATCTTTTAACTTAACACCAATATCCATTAACTTCTTTATTTCATATACTAAGTATTGATTGGAAGATAATGTTTGGTGATTAATATTTTGATTATCAATAACAATTACAAATTCTCCTTTTAAAGTGATGTCAGGTAAAGTGTTTTCACTGGTGTTAAATCAATAATGTGATTCATGTAACTTAGTTAATTCTCTTCCTATAAAAACGTCATTGTTTTTAAAAACATTTTTCACAGTTTCTAAAGTATTTTCTAACCTATGCACTGCTTCAAAAAAAACGATAGTGCTTTTCTGATTTTGATAGGTACTTAAATAATTTTTGAGCTGATTTTGTTTGTGACTTAAAAAACCTAAAAACAAAAGTGGTGTTGTTTTAAAACCACTAGTGATCAATCCACACATTAATGCACTAGGACCATTAATAACTTCGATTTTTATCTCCTTATTTTTTGATATGATCCAATTAATCATTTCATTACCAGGATCAGATAAACTAGGATAACCTGCATCACTAACAAGACAACACTTAAAGTTAGTTATAAATTCCTCAGCAAAAGTTAAATTCTGTTTTTCTTTAAAACTGTTGTTAATAACAAACTTTTTTTGCTTGCAATCAATGTTGAGTAAATCCAGCATTTTTCTTGTTACTCTACTATCTTCACAGAATAACACTTCACAATCTTGTAACGCTTTTTTAGCTCTTTCACTTATCTCTTGAATATTACCAATTGGTGTAGCAACTACTTTAAGTGTTTTCATACATCTTGAAAAGTTAGATTTAATAAGTTAAGTTTTTTAAATAGTTGCTTGTGGTTAAAATAAGGTAAATTAAGATTATTGCATAAAAGTAATCGCTTTTTCTTATCATTTAACTTTAAGCTTAAATACTGATCTCATAATATGCTTTGATTAGCATTTTTTGTGATTGCAACACGATTATTTAAAGCAGCAATTAAAGCACTAGTTTCCATTTCAGCAATACCAGCTTTTTTAGCGTTTTTTAACCTTGCATTCATATCAACATAACAGTTGTAATAGTTTGTTAGATGTTGTTCTAATTTACTTCTAATCTTTTTGCCTTGTTGATCTGGATCTAATAACAAGATAATAGTTTGCTTTTCACTGATTTTTTTAATTAGGTTAATAGTTTCTTTTTTTAAAGCTGAACCATTTGTTGTAATTACATCTACATCAAAAATTTGCTGTAATTTAGCTTGGTCAGTTTTACCCTCACAAACAATTACCCCATCAATTTTTATACGTGCTTTTTGATCCATTCAATGTGACGTTCATATATTGCAGAAACTGATAAGCTGTTAGCGTAACACAGAACAACCTCTTCATATAAAGATGCTAGATCAACTACTTCAAACTGCTTTACAGCTTTAAACTTATATTGAGGAATAGAGTTTGATACAAACAAGAAATCAATTAGTTTTTGATCAAATGTTTCCATAAACTTTTGTTCTGCATCATTGTTAAACAAACCATGAGTTGCCATTACACACACTTTTTTAGCTTGTTCTTTTTGTAATAGCTTGGCTGCTGCAATTACTGTACCACCAGTATCTATCATGTCATCAACTATTAAACAGTTTTTATTTTTCACTTCACCTAAAACATTAATTGATTCAGCAACATTATGAGATGGTCTTCTTTTATCAATAATGGCTAATGGTAGTTCTAGTGTATTTGCAATTAACCTTGCTCTTTTAACCCCACCATAATCAGGGGAAACAACCACCAAGTCTTTTTTACCAAGTAGTTCTATAACTCTAAAAAGAAAGATGTGATAAGTTCTTAAAGAATCAACGGGAATATCAAAAAAACCTTGGGTTTGATCACTATGAATGTCAGTTAGAACAACCCTGTTAGCACCTGCTTTTGTTAACATATCAGCAATCAATTTACTGGTAATTGGTTCTCTTCCTTTTGTTTTTCTATCTTGTCTTGCATATCCATAATAGGGTAGAATGGCAGTAATACTTTTAGCACTACCTCTTTTCAATGCATCAATAGCAATTAAAAGTTCCATTAAGCTATCGTTAACATTAGGACAGGTTGATTGAAAAATATAGATATCTTTGTTACGAACTGATTCATCAAAACGGATATAAGTTTCACCATCAGCAAAGTGTTCGCAAACCATTTTGCCCTCTGAAATATTTAGTTTTTTGCAGATATTTTCAACCAGTGTTTTGCTTTTAGATAAACTAAAAATAACGTGCTTTTTATTATCGATACTCAGCTTCAAGCTCGCGTCGGATTGTTTTCTTTTTAATAGTTTCACGTTTGTCAAATTTCTTTTTAGGTTTTGCCAATCAAATTTCCACTTTAATTTTGCCATTTCTAAAGAATACTTTACTTGGGATTACAGATAAAGATTGTTGTTGTTTTTTATTGATAATTTGTTTAATTTCATGTTTATTTAATAAAAGTTTCTTAATTCTATCTGAAGCGTGATTTAATGGACCTGCAAAACTATAAGGTGGAATAGTGAACTGTTCTAAAAAAAGCTCATTGTTTTTAAAAAAAACATAAGCTTCTTTTAAACTACCTTGACCTAAACTTAAAGCTTTAACTTCACTTCCTTTTAAAACTATTCCAGCACAATAAGATTCCATTAAATGATAGTCATATTTAGCCTTAGGATTGTTAACAAGAATTAACATTTATCAATGAAATTATCTGTAATTATACCTACTTACAATTGTGCATCATTTATTGAAAAAGCAATTAATTCAATTGTTAAAAATAGACCTAATGATTTGGAAATAGAAGTTTTAATTATTGATGATGGATCAATTGACAATACTAACAAAGTTATTAAGAAAATTCAAGACCAAATTAATAATTTAACTTTGCAGTATTTTTACAAAAGTAATGGTAACTGGGGTAGTGTTATTAATTATGTTAGAAACAATAAACTAGCAAAAGGGGAATGAGTAACAGTATTGGATAGTGATGACATTTTTTCAAAAAAAACAATTTCTATTTTTCAAAAATATGCCCAAAAACAAAGATATGATGCGATTATTTTTGACTACTATAAATGCTGAAAAAAGTTTTTGTGAAAAATTCCTACCTATGCAAGGTTTAGAAAAGAAATTAAAGGTGAATTGAAAAAACAAACACCTTTTTGTATTCCCTTAGCTAAGTTTTTTAAAAATGAGGTTTTCTATCAACTTCCTAAACTAAGAGAAAATGTTGGTTTTCAAGACGCTATTTATACGATGCATGCATTACAAATTGCAAATAATGTTTTCCATGTTTCTAAAGCTGGAGGATATTACTTTTTTAAAAGGGTAGGTAACTCTATGAGTATCCCTTGACACAGTTCTAGGTTTGATATTGAAGTACAAATCTGCAAGGATCTGATTGAAAATAATGCGCAAGAGATCACTTTAGTGCATTTACTTCGTTTAAAATTTCGTAATTTAGTTGATGATAAAAAGATTAAATTTACAGTTAAAAGAGACTTTTGTTTTAGTGGTTTTAGTTGGTATAGTAGGTTAATTTTATCTCTGATGTATAACTTCTGATTGAAACGTTATTTCAACAGTTCTGAATAACGATTTTTTTGCTTATTTGCAATTACAAACTGTCATTCATTGAGTTTTCATTTTCTTGTCATTTCCTGAACCAATTGATCACCATCTTTAATCCAACAAGCAGGAAAGAACTTAACAATTAGGTTTGCTAGTGCAATAAGTCCTAAGAAGAAAACAATACTTACAATTACCCCTGGTAAAATACTTGTACCTTTTCCTTGAATTAAAACAGGCGCTTGGAGAAATACTGATTGGGTAATGTCAAATAAGGTGTAAGCAATATAACCAAAGCCCCAACAAAATCCAAAAAGAATAGGAAGTTCATTCTTCTTGTAATCTTTAAATTCATATGGCAGGATTAAATTTGAACTTGATAAACTCCAAGCAAATGCACCACCGATAAATGAAAAGATACTAATTAAAGCAAACCCAGCTGCACTACCAATACCAAGTGTTGCTGCAAACATGATGATTATAAGCAATACCACTATGTTAGCTGTTAATAAGAAGTGCATTCACCTCTTCTTGTCATAAATGGTTTTATTAAATGGTGAAAAAACAACAAAGCCCAAAGCATAACCAATAACTCAAAAAATAGCAAGTGTTGCTAAACCTGTTGTGTACACACCAGTACTTATCAAACCATTTGATCCTGATGGGGAAACAGTTTGCAAGATGCTAAATCAAGCTGGAGTTAACGGATTGACTATTAGTATTAAAACTATGCCATAAACACCAATTAATTTTCATGTTGTTTTATTCTTTAAGATATCTATGGATTTTGGTTGAGTAGTTAAACTCATATTTTCCTTAGTTTGCTTTTGAGGAAAGATGTGATCTATCTTGCTTTCAAACCATAAAAATAAACATAAGTTAGCAAACACAACCAAAATCATCACAGTCATAATGTAAACCCAGTATTGAGATGCTACTTGTTGAACTTGTTCAAATAAAAATGGTGTGAAAACTACTGCAATCCCAATGTTAAAACCCCAAAGGTTAGCATTAGATAGGATTGATTTTTTTCTGTTTGAAGAAAGATTAGCAATTGCTGGTTGAGTATAAACTACTAAGGTAGTTCCACCAATAGCAATTGTGCTTCTAAAGATAATAAACAATGCATAACCAGTAATTGATGCAGCTGTACCAATACTATTACCATCCAAACCCTTAATAAGTTCAACACTTGTTCCATCTGCAAGCATCGTTTTACCATTGATCATCACCGGTCCAAGTAATTGACCCACTTGTAAATTGCTACTTAGTGAACTTAATTGAGTTTTTACTGAATCTGATAAAGGTCTTAATAAAGTTAATTGATTATGACCATTCAAAGGGTCTCCTATAATAAGAAAGGGAAAACAAACACACATGATTCCCATCATGATTAAAACCGCATAACGATAACCAAATTTCAAAACAACAACTCCACAAAGAATAGAACCTACTGCTCTCAATAAAGTGATAGTTCAGTTGGTGGAAGATGTGGCTATTTGCCCAGCATTTTCAGTAAAGAAAGAACTTAACCAACCACTGTACTGTGGTAAAGTGGTGGTTGATTGAGTAAGTATACCAGTTGGTTTACCACTAATTCGATCAATTACAAACCATTCAACTACAAACAATAGATACCCAAAAATTACTATGATCCACAGGGTAATAAGTTTCAGATCACTTACTTTTTTTTGACTTTTATTTTCCACAAATTAAGACAGAATTTACATTAGACTAATTTTAAAAGCTGTAGCTAATTGTTTTTTAGAACAAACTGAATCAAAGAATTTGAAATTGTCTTTCATTGTTAACAGCATTTAACAACAAAATTGAATAAAAATAAAAAACAGACCCTGATGGTCTGTTGGATATTTAATGGCGGAAGCGGTGGGATTCGAACCCACGCACCGTAGAACGATCTAACACCTTAGCAGGGTGTCCTCTTAACCACTTGAGTACGCTCCCAGTTTGTTGTTAAATTTATTATAAATATGCAACTAATTAGCGCTTTAGATGTTTAAAAATTTATTAAGACCATCCCTATTTTTTAACTGGAGCCAAAAAACATTTAAAAATAAATTTTCGTTTTTAAAACAAGCAGCAAATGCATTACAAAAACAGGCTGTTATCAATGATAACAATGTTGCATTTGAAGCTTTAAAAAAGCGTGAAGAAGAGATTACAACTGGAATTATTACTAGTTTAGCTTTACCTCACTTACAAAGTCAAAGTGTTATAGAACCTTTTGTTGCTGTATTTAAGGTTAAAAACTTAGATTGACAATCATTAGATCAAAAACCAGTTAAATTGATATTTTTAATTGGTGTTCCTGTTGACAAAACCAATTTGCATCTTGATTTCATTAGTAACTTTTCCAAGTTAATGTTGAATGAAACATTTGCAAGTAAGGTTTTAAATGTCACTAGCTATAACGGCTTGATTAAACTAATTGATCTTTTTAACCAACAAAAAGTGCAAGACCAACCAGCTGTTGAAACAAAAAAAGAGTATGACTTTGTTGCTGTAACAGCATGCCCAACTGGCATTGCCCATACTTTCATGGCTAAAGAAGCGTTGGAAGCATTTGCAAAAAAGCATAACTTATATGTAAAAGTTGAAACTCAAGGTACAGATGGGATACAAAATCAACTTACAAGTGACGATATTAATAACGCTAAAGGTGTTATTCTTGCTTGTGATCGTTTAATTGATTTCTCTAGGTTTTATGCAAATAAGAATGTGATTGAAGTGTCAACTCTAAAAGCCATTAAAAAACCTGATGAGGTGTATGAATTAATTAAAAACCAAAAGGGTAAACAGCTTGCAAATAGTGCTAAACCAACTAATTAAACCCAATTAGCTGAAAGTGAAGGGGAATTCAACTTTAATAATTTTCACAAGCGGATTTATCGTGCCATTTTAAGTGGTGTTTCTTACATGCTACCATTCGTTGTTTTTGGTGGGATATTAATTGCACTCTCTTTTCTAATTGATATAAATAACGCTAATAATGCTGGTGGTAACTTTGGCACAATTAATCCTGTTGCTAACTGGTTAAATAAGCTAGGAGGAATTTCCTTTAGTTTAATTGTTCCTATCTTATCTGCATACATTGCATATGCATTGGTATCAAGACAGGGATTATTACCTGGTTTTGTTGTTGGTTTAATCTCTTCAGGACAATTTTTATTAAATATTGTTCTGACCAATGGAACAATTGAATGGTTAGCACCATCACAAGTATCAAGTGGCTTTTTTGGTGCTATCTTTGGAGGACTTTTGAGTGCTTGCTTAATAATTGTTCAACAAAACTACATCTATAAAAAATTACCTCAATCTTTGCAGGGAATTAAAAACATCTTGTTTATTCCCTTGTTTGGTACTTTATTCACTGCTGGTTTATTTTGGGTTATTAACATTCCTTTAATTTATCTAAACTATGGATTAAGTCTGTTTTTAAATATTATGAACAGCCCTATCCTAGCACCTTTACTTGGTTTTGTAATTGGGTTGATGATGTGCTTTGATTTAGGGGGGCCAATTAATAAAGCAGCTTATGTTTTTGGTGTTGTTTCTTTACAAAATCAAAATGCAGGAACAATTGCGATGGCTGCAGCTATGCTATCAGGGATGGTACCTCCTTTATCAATTGCTTTGGCAGCTTCCATTCGAAAGAGCTGCTTTGATAAACAGGAATTACCTGCAGCTTATGCTTGTTATCTGATGGGATTGAGTTTTATTAGTGAAGGTGCTATCCCATTTGTTGTTAAAAAACCTAAGGTGATGTTAACTGCTAACTTAATTGCTGGAGCAATTTGTGGAGCATTAACAGGAGCATTTGCCTTATCAATTCGTGCTCCTCATGGCGGTGTTTTTGTGTTTGCACTTTTAAAAACTACTTTACAAGGGATTGAAGGAGCTACATTACAAACTGGAGTTGGCATTGGTTTGGCATTGGTTTGTTTAATAATTAGTATGATAGTTGGTAGTAGTATTATCATTGGCTATGACTTGATTGCAAAACATAACCAAAGAAAGCAAAATCTGAATAGTTAATTACGCTTGCGCAATTGATTATTATGTTGATTTAAACAAGCAAAAAAATAGTGTTTTAATACCTGGTGGTAAGGGGATTAATGTTGCTATTGTAATGAAATCACTTGGTTTTGATCCAACTGTCATTACTTTTTTGGGACAACCCACTAAAAACTTATTTTTAGAGTTGGTAAAACCTTATGATCTAAATATAGTTAGCTTCATTTCTGAAACTAAAACAAGAATTAACCTTAAGTTATTAAAAGATGAAAAAACTACTGAAATTAATGATTTAAGTCCTTTAATAACAGATGCTAATCTAACTGAATTGTTAACTTTTTTAAAAGCTAATGTTAAGAATAATGATTTGGTTATCATCAACGGAAGATTTAAATTTGAAGCTTTAGAAAAAGTTCTAAACTTGGTCTTTACATTAACAGAAAATGTGGTTATAGATGTTGATGAAAGCAAAATGTTAACGCTCTTAAATCAGTCTAAACCACTAGTTATGAAACCTAACATTGATGAGTTTCAAACTATGATTAATACTTTTTTTCAAGATCAACAAAGCTTAATAGCAGCAATTAAAAAATTTCATTACTGTAAGCTCTTGTTATTATCTGATGGTGACAAAGGAGCTTATCTTTTTGATCAGAATAAGTTATTGTTTGTAAGTTCTATCACTCCTAAACAAGTAGTTAGCACCACAGGAGCAGGTGATACTTTGTTGGCAGTTTTTTTAGCAAATTTGATTCTAAAGGTAGATTTAAAAACTGCTTTGATTAAAGCAACTAACTATGCAAGTGCAACAATTAGTAAGTTAGGTGTTGTTGATAGTAAAGACAAAATTAGTGTTATAATCCCAAAAAGTTACTATTTATAATTAATATTTGATGGTAAAAAGAAAGCGAAAGCCTAAGCTTAATTCGCGTAATATTTTAACTATCCAGATTGTTTTAGCAATCTTTAGTATGATCTTTTTTCTTACTTTGCTATCTTTAATCTTGTTTTTGAGTTTGCAAAGTAATTTAGCTACAGCTTTAGTTGAAAACAGAAATAAAGCTGTGGAACTTGTAGATAACATTGTCTTTTTTTAAAAGAACTGATTTTTAAATTTGTCTCTGACTAATTTATTAGTGTGTTAAACCTAATTAAAAATGTTATTCGTTCTTTAAAGAGTGCTAAGATTGCTTTAATAGCGTTAACTTTTTTAATTTTTGTTGCTGTTGGTGGTTTTGTGTTGTTAAATAACACAGTTAATAATTTTAACGCTGCTTTTAACTATGTTACCCACACTGGTAAATTAAGTAATGCCATCATTAATGAACGTTATGACTTTGGTAAATTAGAGTTTCAAGAACAGACCAATAATTCTCAGAATAGTAGCGACAGTTTTACTTTAACTTTAACTAACGATTCAAGAACAAGTTTTATTAATAATGCTTTGAGAACTAACCCTTCTTTGTATGAAGGATTAGTAACCCAAACTTTTAGTTATCAAAACAAAACTGAAATGACTGAAAAAACCAATATAGTTAATCAGTCTAAAATTATTGCTGCTAACAATCTTAACAATGCATTAAGTAAAGATAAACAGCTCTTAGTTTCAGGCCAACTTGAAAAACTAAATGCTGTTTTTCGAGAATATAAAGCTATTAATATTACTGACAAAAGTGTTTTTAAAAAATTGATAGTATCAGAACCTAATGATTTGGTAAATAGCCTAGTTATTTTTGATGGTCAAAATTTATCTCTCTCCAAACAAAGTTATTTCAATAATTTTTTAAATCAATTTAACGAAATTAAATCAAAGGGTAAAGATAATTTAAGTACTACTTTAAAAACTGGGCAGTATCAGGCATTTTTACAAACTCTTTTTGATTATGTTCAAGCGAGTGAAACAACATTAAAAGATCAGCTTCAAAAGTTAATTTCAAATCCAGATTCAAGTGAAACAAATCAGGTTAAAAATCTCTTTGATACTCCAAGTGCGCTTACTAATATTGGGGGTCAATTAACCTTACAATGAACAGAAAATAGCCTAACAAAACAGATAGTTATCTTTGATCCTAGTAGTTATGAAACAATAGTCGCTCCTGGTAACTGAACTTATCAACAACAATTAGGTAAAGAAGTTTATCCTGATATTAACAACTGAGAAAGTATTAAAAAACTACCACTTGAACAATTTGAAAGTGAATTTTTAAAAATTGATCAAAAGTATAAGATCAGTATTGATAATATCGATTATTTAGTTATTGGGGTTGGAATTAGTCCAGATTTTGTTTATCCTGTTTTTAGTGCATCTTTAATTGTTCCTAACATTGAAAATGAACAACTTTACTATGTTAACCAAACTGGATATGAAAGAACTTTTTCCTCTTTTTTAACAAATCCAGTTGAAACAGCAATAGTAGCAAGATTGATTAATCTTGAAAGTGATCTTAATACTATCAACCAGTGAGCTGTTGAAAACATGTCATGACCAACAAACATTAAAGCTGCATACAGTAGTTCTGATACCACTAATATTTTGAATTTATTAGCAGCAAGAACAGTTTTTATCCCTAATCTAATTAACACAATTAATTTAGTGGCTTTGTTTTTAACTATTGCTATCCTAACTGTTGCTATAATTGTCAGCATTTTAATCCTGATTAGTTATTTAAAGAAAAACACTGAGCAAATTGGCATTTTAAAAGCAAATGGGTTAAGTGGTAAAAAGATTAACCTTAGCTTGTTAATCTTTGGGTTAATTCCTGCTATAGTAGGTGCTATTTCTGGATATAGCTTTGGAATTGGATTTCAAGACGTAGCTATTCATCTATTTAGTAACTATTGATTTATACCAACAGCAACATCAAGTTTTTCAGTAGTAGGATTGTTGTTTTTTTCACTGTTTGTTATCTTAATTATGAGTAGTATATCGCTTTTAGTGGGATCAATTATCTTAAAGAAGGATGTTGTAAAGATTTTAAAGCACGACAGTGAATTTAAAGTTTCAAGATTAGGGCTTAGTTCTAAGAAATTGTTTGCTAGGTTTGGTATTATGACCAGGTTTAGAGTAGCATTAGCATTTAACGCTCCTTGAAAATTAGTTTTTCTAACCTTGATGAGTTCATTTACAATGATGATTTTAAACCTTAGTTTTGCAACTAAAGATAGCTTTGAAAATGCTCAATCAAAAACTAATTTAACTAATCAGAACCACCAATATGAATTTGAACTCGCTTCAGCAACAACACAAAGTGGTTTATTGAAGTGACAGTTATTTGCAGAACTAGGTACAACTGATAAAAGAAGTGAAAGTAGTGTAAAGCTTGCAAATAAAAGGATGGATATTAGTAATGTTGATGCATCTAAAGATTGAAAGAACCAACAAGTAATTAATTTTTTAAGCGATGCTAGTGGCTTTAGTAATGATTTAAATTACCTTGAAAACATTGTTCAATCCAAGATAGGTTTGGACTATTCATTGGGATTTAACAATATTGTTTCAAATCCCTGAAGGTTAAGTGAAACATTAATGCCAACTAACCAAGCATCTGCTTCCAACACTGCTTTTCAAAATTTTTTAAAAGCAATCATTACTATAAATCCAAGCCAAGGATCGCAATTCATTAAACAAACCCAAGATCCATTAACAAAAAGATTTATCTATGCAATTGACAGTGATAAGGCATTAAAAAATAATAATGAACAAAACGGTTCCCAAAACCACTTAACTTTAAATGATGATTTTGCTAAATTTCTCTACAGTCAATTTGAATTAATTAAAAAGAGTGGGAATGCAAGTAATGAAGATTTAAATGCAATTGATTTTGAAAACCCCCAAACAATCAGAGATTTTTACAACAAGTACAATGCTTTACCACCATTAGATTACAAACTTAGCTTTAATGTAATAGGTTTACCCAAAGAGACAATTGCTGGACAAATTGACACCCCTAAGTATGGATTTTTAACCCTTCATGGTGAATATCAAAATACTCCTATCAAGATTAAAGGTATTAAAGATTGAAAAGATAAAGTGGATAATTTAGGTCCAGTTTTGAGTGATCAAAACAACCACATTATTAATCAAGAATTGTTTAAAAATTATTCTTTTGATCCTTTGATAGTTAACAATTCTGCTGCAAAAAAATACCAACTTGCAATAGGTAGTGAGATTAATATTGCAGTTAACAACAGCTTCAAACGGATTGACAATAAGATCATTAATCAAGATCCTTTAGTGAATGCTACCTTTAGAGTTGTAGGGATTAACAATTCCGCTCATGATCCTGAATTTTTCACTAGTTATAGTACTGCTTTTAAAGTATTGGAATATCCCAATGAATGGTTCGTAAAAAAACTTCCATTTAATAGCTTCTATGCTAATTCGCTTTTAAGTTTTGTTCAATCTACTTCGCTATTTTCTGAATCTGGTATTTTTCCTGCTACTAGTAGTTTTTCAACTAATAACACTGTACTTGTTGAGTTAATTAAAAAAACCATTAATTACAAGAATGGTCAAATGAATCAAACTTCAAGTAATGACTCTTCTAAGAAAGAAAATTACCAAAAATTGCAAAAAGCATTAGGAATATCAACTGATTTGGAGATTAGTAAAGTTAATGAATATGTTGCTATCTTAGCAAGGGTTTATAATGGTTTACCTTACAACTCTACTATTAGCTTTATTAGCAATGTTGCTGCTAACAACGCTTTATTTGGAAATATTGCTAACACCACCAAGCAGATTCAAGCTGTTGTAATTGCAGTGATTATTCCTATAATCATGTTGATTATTCTTTTGGTTTCAACTACCTTAATTCAAGAGTTGAAAAAAATTGCTATTAGATTAAAAGCATTGGGATATTCCAATTTAAAAATTCTCGCTTCATTTTTATCAATATACATCCCTTTATTTGCCTTTGGTTTGTTGATTTCTATCCCCTTTTCTATCTATCTAATTGCACTACATAATGAGGTAATTTTTGCAAGCTCATCGATCTTTTTAGATGCTTTTTTAAGTTTTGAAAGTGCAATTGGTTCAATGTTAGTTTTACTAGCGGTTTTATCAATTACCTTTGTGTTGAATTGATTAGAGTTGAACAAAATTAAGATTGACAAGGAAATCAAAAACTCCTAATGGATTTTTTCTCTTTAAACAAAATCATAAAACCCAACCAGAAATTCACTAGTAATGAAGCTGAATTTCTACAGATAGCTACTGATTATTTGGAGGAAAGTCAAAACTATCTTCAAAAGGGTTTAAAGCAATTAAAAAAAGAATATAAAAGATCCATTATTTATAACCCTAACCTTGAATATAAACGCTTTGTTAAATGAAAAGAAAATTTCACTGAAACATTTGAAAGTTATTATGACAGGTTTTTTATTACCAAATACAACCATTATTCACTAAGCTTACTTTTTAGCTTTATTAATGAACAGATTGAAACAGTTATTGCTAGTTACAACTCATTTCTAAATGAGCATAATAAGTTAGCTTTTAATAAAGTTAGTTTTAGTTTTGAAAAGAAACTTTTTGAAGCTACACAACAGTTTAATAACTTAGAAAAAAACACTGCTATTAGTGATGATTTACCGCTCCAGTTTAAAGTTAGAACAACTCAACTAAAAGCCCAAAGAGAAAGGGAATTGAAGAACTTGTTGAATAAAATCAAGCTTAAAAATTTAAGTGAAAAAAAACAAGAAATTTTGTTAAATAACTGGTTTAATAGCAACGAACGTTTGTTTTTAAAAAATGAAGTGAAAAAGGTTAATTGACTAAACTCGCCAAGACAAAAACAACAAGCAGCTCAAATTGATGATCAAAACATTATTGAATTGAAAAATGTGTATAAATACATCACTAATGGCATTACTACAAATGCAGTTCTTAAAGGAGTTGATCTTGCCATTAAAAGTCATGATTTTATTGTGATTTTAGGCCCTTCAGGATCTGGTAAAACCACATTACTAAACATTATTTCAGGGATGGATAGAGCTTCTAGTGGTAGTGTTATTGTCAATGGTTATAACATGATTTGTTTAAATGATAGAAAGCTCACTAAATTCCGTCAAAAGTATGTTGGTTACATCTTTCAACAATACGGTTTATTACCTAATTTAACAGTTAGAGAAAACATTGAGATAGGAGCAAATCTTCAACCAGATCCTAGTAAAAGGATCAGCATTGATGCACTTTTAGAAGCGGTTGGGATGGATAGTTTGCAAAAGAAGCTTCCTAATGAATTGAGTGGTGGGCAACAGCAACGTGTTTCCATTGCAAGAGCTTTTGCTAAAAACCCCTTATTAATTTTTGGTGATGAACCTACTGGGGCACTTGATCTTGAGATGACCCAAATTGTTTTAAAACAGTTTTTAGCAATTAAAAAGCGTTATCAAACGACAATGATTATTGTTACCCACAACAATTTAATTGCTAACTTAGCTGATTTAGTTATCTATGTAGCAGATGGAAAAATAAAATCACTACACAGAAACTTAAATCCTAAACAGGTTGAAGAGATCCATTGAATTTAAATTATGAAATACTTGTATGCCACTCAACACCTTACTTTAAATGCTATTAAGCATGCTAAGGGAGGACATGTTGGCATGGCCATTGGTGCAAGTCCTATCTTATTTAGTTTATTTACTAAACACTTTCACTTTGATCCTGACCAACCAAAGTGGATCAACAGAGATCGCTTTGTTTTAAGTGCTGGCCATGGTAGCATGGCATTATATTCAATTTTCCATTTTGCCGGACTTATTTCTAAACAAGAGATCTTACAGCATAAACATGGTCAAATTAACACTTCTTCCCATCCTGAATATGCTCCAAATAACTTCATAGATGCATCAACAGGCCCTTTAGGTCAAGGCTTTGGCATGGCAGTTGGCATGGCGTTAGCACAAAAGTTATTAGCTAATGAATTTAAAGAGCTAAGTGATAAATTGTTTGACCATTACACCTATGTGGTTGTTGGGGATGGAGATCTACAGGAGGGGGTTAGTTATGAAGTTAGTCAAATTGCTGGGTTATATAAATTAAATAAACTAATTGTGCTTCATGATTCAAATAGAGTGCAAATGGATAGTGAAGTAAAAAAAGTTGCTAATGAAAATCTAAAGGTTAGGTTTGAAAACGTTGGTTGGAATTACATCCATACTGATGATCAACTAGAAAATATTGATCAAGCTATTATTAAAGCCAAACAATCAGATAAGCCAACTTTTATTGAAGTGAGAACAACTATTGCTAAAAACACCCACCTTGAAGATCAGTATGGAGGACATTGGTTTATTCCCAATGAAGTTGACTTTCAACTTTTTGAGAAAAGAACAAATACTAACTTTAACTTTTTTAATTATCCAGATAGTATTTACCAATGATTCAAACAAACTGTTATTGAAAGACAAAAACAAATTAAAGAAGATTACAACAATTTGCTAATTTCTCTTAAAGACAAACCACTTTTTAAAAAATTTACTAATTGGATTGACAGTGATTTTCAAGCCCTTTATCTTAACCAACTAGATGAAAAGAAAGTAGCAAAAAAAGATAGTGCTACTAGAAACTATTTAAAAGATTTTTTAAACCAAATTAATAATCCTAATTCCAACTTGTATTGCTTAAATGCTGATGTATCACGTTCTTGTTTTATCAAGATAGGTGATGATAATCTCCATGAAAATCCTTGTTCTAGAAATATCCAAATAGGAATTAGGGAGTTTGCAATGGCAACAATAATGAATGGTATGGCACTTCATGGTGGTATTAAAGTGATGGGTGGTACTTTTTTAGCATTTGCTGATTATTCAAAGCCAGCAATTCGCTTAGGTGCATTAATGAACTTACCAGTATTTTATGTTTATACCCATGACTCTTATCAAGTAGGGGGTGATGGTCCTACTCATCAACCCTATGATCAACTACCAATGTTAAGAGCAATTGAAAATGTTTGTGTATTTCGTCCTTGTGATGAAAAGGAAACTTGTGCTGGATTTAACTATGGTCTTTTAAGTCAAGATCAGACAACTGTTTTGGTTTTAACACGTCAACCCTTAAAATCAATTGATAACACTGATAGTTTAAAAACACTGAAAGGTGGCTATATCCTTTTGGATAGAAAACAACCTGATTTAATTATTGCTGCTAGTGGTAGTGAAGTGCAACTTGCAATAGAGTTTGAAAAAGTTTTAACTAAACAAAATGTAAAGGTAAGAGTTCTGTCAGTTCCCAATATAACTTTGCTTTTAAAACAAGATGAAAAATATCTAAAGAGTTTATTTGATGCTAACAGTTCACTTATCACTATAGAAGCGAGTAGTAGCTATGAATGGTTTTGCTTTAAAAAGTATGTTAAAAACCATGCTCACTTAGGAGCTTTTAGTTTTGGTGAATCTGATGATGGAGATAAAGTTTATCAGCAAAAAGGGTTTAATCTGGAAAGGTTAATGAAAATATTTACTTCCCTAAGAAATTAAAATTATCTTAATGTTGTATAAGTTTTGAAAAACAGGATTGGCAATTTTTATGCCTGGTTGCATCTTACTTTCATCCTGTTCTTTTAGAAGTTATATCCCAACTCCTAGTTTAAGAAATACTGTTGGTAACCACAACAGTTATGTTAATAATACTGTCCCTAAAAACAATTTTTATGAAAAGTTTTATGATCTAACTTTTGCTTTAAATTTCACTAATCAGAAAACTCAAGAGTTTGGTACTGGTTGGTTAATTGACTGAAAAGGAGATGAAACTAAAGATCTTAATACATTAACTATTGCTAGTTCTTCTATTATTTCCTCTGTAAGTAATCATTCTTTAAAAGAAAAACAAGATGACAAGCTTTTTATTGCTTATATTGCCACCAATTTACATGTGATAGACGGTTTAAAGAATGATCATGATTATCAACCATATAACAAAGATGGAAATGGTCTTAGTTTTCCATTTGATCAAAAAACCCAATCATTCTTATTGGGTAGGTTTGCCAATCCTAAGATAAATTCCAAACCAGAAGAGATGAACTACCAAGTTCAAACTCGTTTAAAACAAGATGCAATGGTGTTTATCCAAACCAGTACTTTACCTAAAACTGCTTATGCAGGAATTGATCCTATTAACTTTGATTACCATGAAACTAGTGATGAGAGTGGATTTTGAACTAAAAAACAAAGCACTGCAAACTTCCCTAGAACAAGAACATTAAAAAGTTATGCTGATTTTGCAGTTTTAGAGGTACCCTTATTCTTAGATAATGCTAATGATGCCAAGATTTATCAAGAGTGAATTAGACCAGCAGTTCAAGCTTATAAGGAGCTAGGGGATGTTGAAAATATTTTTGCTAAAACCCCATACGCTGAATATATTAATAACACCTACTACTTATTGGGTTATCCTGTTACTAATAACAATAAGTATCAGTTTATCTTAGGTCAAGATGAAAAGTGAAAGTTTTCTCAACAAACTTCTGTATTAAAGCACTATCAAAAACAACCTTTTCAACAAAGAACAGTTTATGTTGAACGTGATGATGGTCTTCCTACATTAACTTTTAATGAAGATAAACTCACTCATGTTCAAGGTACTGATCTAATTAATGTTGATCAGATTACCGATACTAATTTAGGAAATGGCTTAATAAATTATGCTGGTTTATCACGCTTTACTTTAAGCTATCACAATGTTGAATATAAGTTATTTGGTTATGGCACTATTTTAAATAATACTAATTTTCCAGGTGGATCTTCTGGTAGTGCTGTTTTCAACAAAGAAAAACAACTAACAAGTATTTACTTTGGTTCACTGATTAATGTAACAATTGGAAATAACAGGAATGTTAATTTAGGTTTGGGTCAAATTCTTCGTACTTACAACACTAATAATAGTAAGCACAGTGCACCATCATCATATGATTTAATTTTTGGTGATAAAAACACCATCAAGTTTTATGCACAGTTTGCAAAAGAAAAGCAAACTCATCTTTGAAATATAATTCAAACATCTGTTAACTATTCAATCAGCTTTTACAAAGACAAAAAATAATAATTAACTAACAATTTGTATTTGTGTTAAGACGTTATCTAACTCTGAGTTTTTCTAGCTTGCTTTTATTAGCACTGCTGTTTTTAACAGGCTGTTCTTTTGTTAGACCACAATTTCGCAGGGGCTTTAGAACCCAGTTTAAAATTAACTCTATTCCAACTGTTAGTGATCCTTATCACATTAACTACGACTTAACTTTTTCATTAAACTTTGCTTCCAACAAAAGAAATACTTATGGTACTGGTTGGTTAATTGATTGGAAAGGAGATGAAAATAACCCTGAGAAAAATGATCCTTTTAAAGTTTATTTAGCTACTAACCTCCATGTGATAGATGCGTTAAGAAATAATAATGACTATGAACCATATAACAAGGATAGTAATAGTCAAGCTTTTAATGGCGAAGAGATCACTAGGTTCTTTTCTATAGGTAAGTACACATATCCTAGTATTTTTAGTGAATTAAATTTCATTAGCAATGCTAGAGAAGCGTTTGTTTCGATTCAAACCTCTACTATTCCTAAAACTGCTTATGCTGCTGTTAACTTTGTTGAAACACAAGGAGAGGATGAAAGTTATACAGATTCATTATCAACTGATAATAAAAGAGATATTTATGCGGATTTTGCTGTGATAGAAATCCCCTTATTCTTAACTAACCATCGCGATTATCAAGTATTTAATGAATTTATTAAACCAGCAATAGAAACATACAAACAACTAGGAAACTCTCCCTTTGAAGAAAAACAACTAGATCAGCATAAAAACGACAACTTTTACATGTTAGGTTACCCTTTGGTTGAAAGTAGCATAGATGCTCTAATTTTAAACCAAAGAAGACAATACAATAACAGTTATACTGAAAAATATACTCCTCAAACTTTGACCAAAGACCAACGCACTATTGACTTAAGCAGAGAAGTTCCTACTTTAATTCAGAACAAAACAGAAAATTCTACAGGAAGTCAATTACTAGTAAATCAATCTTTAAGTAGTACATCTGAAGGGATTATTGAATTTATTAAGTTACCTGAATTTAAACTCAATTATCATAATAAAAGTTACCGTCAATATGGTAGAGGTTTTGCTCTACAAAACACTAACTTTAGACCAGGTTCTTCAGGAACTTTAATGTTAAATAACCAAAAACAGATAGCAGGTATTTATTTTGGTGTTTTAGATTTTGGAGAAGATGTAAGTTTAATGAGTAACATTGGCGTTGGACAAATTCTTCGTGTTCCTCAAAAGAACAATACTAGAAATAGATCAATTGCTACTAATAAAAGCAACTATGATTTGATCTTTGGTGATAGCAATACAACTAACTTTTATGCAAAATTTGCTAGACAAAATAACACCCATCTTTATCAGATGATTTCAAATAGTAAAGATACAAAATTAAAGTATGTGAATACTGTTGAAAAAACAGTAAAAGCTAGTATTAAATAAAACAAAATAGTGACTTTCTTTTTTTATACTCATAAAAATGGAAAATCAAAACAGTTGTAGTTCTGTAAAAGAAAAAGAGTTATATAATCCAAAAAAGCCTTTTCTTTTTGCGGTAATTTCTTTCTTTGTTTTCATAATATTTTTTGCTTTAGCAGGTTCATTTACCAAATTTGCATTAGAAGAAAGCAGGGGTTATAGTTTTCTTGTTATTGTCTGTTATCTTCTAGGAACAGTTTGTTTTTTGTTTTTATCTATTTTTGGTTTTGATTCTTATCTGAATTTTTTGCAAATAGAGTTCTAGTTAAGTTATGAGATTTACCTACTAAAAAGAGAAGAAAAAGACATGCAAAAAAACAAAGATAATTAGTTAAGTTTTAATAACTCAGTTGGCAAATAAATTAGGGAGTTATTAGAACGCTATTTTTTATTAATTTAAGTGTTTGTTTAAAATTTATTAAGTACTTTTTTAACTTGTTAAAAAAGTAGTTATTTTATTAGTTGGAAAGTGCAAAAAGAATTAAATATTTCAAATGATAAAAAGACTTGTTTAAATGAAATAGAAACAAGCAAAGTTAAATTTCTAACGACAACTTTAATTTTGTTGTGATCAATATTAATAACACTCTTAGTTGTTGTTAGTGTATTAGCATTTAATTTCATTTTATTAGGATCTACTAAACAAGGATTACCAAATACCAATGAAATAGAGACACTAAAAAGTTCATTAGCCTTGCAATTATCACAAAATGGTGTGATCTTATCAATAGCACTACTTGCTTTTTTTAGTTGGATGGCAATTGTTGGAATTCACAGCTTTTTAGTTGGAATTTTAGCTAATCATCAAACACTTAAAATAAGTAAAAAGTATGTTATTTTAGGTTCGATTTTTCCAATTATGGCATTAACAAATACTCTTGTAATTAGAAAAAAATTAAAAGCTTTATTAGGAGAGGGTAAGGTTCAAAAAGGACTCAAAGTTTTAACTATTAGTTTTATTTGTGTTTGAAGCTTGCAATTGATAATAGGCTTTTTTGTATGACTTTTTCCTTATGTTGGTGAGGCAGGAATTAATATAGGAATTAATTTATCTATTTTTAATTTGGCTCAGTTAGTAGGCTCAGATATAAATGTAATAACTTACTTTACAACTTTTTTAACGCTGCTTTTTGCATTTCTTTCATGAGCCGTATTGCATGTTTTAGCTTGCTTTATTTTGGTACATTTGACTATTTATAAACAACAAAAATGAATAAAAATTAAAGCTATTTACATTTTGGCTTTAACCTTAATTGATTTAATGGGTTTAATTTTGGCTTTTGGTATTATTGTTGTTAACAATACAAATTCTTCAAGTTTTGATGCAAAATCAAATTCCTTAGCATTAATTTTTAAGTTCATTTATTTTGATAGTTCTATAGCAGCCTTTTTAACAGTTTTAAGCTTTGCTAGTTCAGTTGCTTTACTTGTCAATTTAATAAAAAAAGATAAGGGATTAACCTTAAAAAATTAGTTAACCTCCCCCAACCTCTATTTTGTGTTACTAGTGCCAAAAGTGGTATTGGAGTATCACAACCTGAATAACCAAGTAGTCAAAGAGAGTTTGGAAGTGGAATCTTCTTCCTCCTTTAACCCCACCCAAAGGTTGAAAAAAGATAGTCCAATGAAGGATTCAAGTAAACACAGGGAGAAACTCACCGAAACAACTTCATCGATGAGTAGTGGTGGGGCTACATCCACTCGAGCCAAGGCCCTCAAGGTTGAGGTGGAAAGGGGGAGTCAAAGTGATTCACTTTTAAAAAACGACTTTGCCAAAAAGCCACTGAAACATAAAGAAAATAGTGGGACAGATGTGAAGTTGGATGCGAGTGGGGAGTTTGCCAATGATAAAGCCTGAAAACCGGTGTTGACTACCGAGCAAATAGCAAGAGAGAAGGGGATGGGGGCGACTTAGACTTTCTCCCCTGAATCGGCAATGGTAAACCCTTCTCCAACTCCCCTTCTTCTTCTACTTCTACTTCCTCTTCTACCCCACTCCCCACTTTTTCTAACATCAATGTTGGGGTTAAATCTGATATCACTAAACACTTAAATCAGCAAAACACCCGGTGGGTGTTTACCCCTAACTCTTCACCAGACATCTGAACGGGAGCAGGGTATCGCAAGGGCTAATGACAACAATAACGGTATCTCCTTAACAAGTGTGTTGCCTAGTAGTAGTAGCAACACGTTTAATCCCAATTCAGATGATAATAAAGTCACACCATCAGGTGGCTCCCCCAAAAAAAACCACCTATCCTTTTTTACCCAACAGTATCAGTCCCACCAGTGACTGGATCAATGCGTTGACTTTCACTAATAAGAATAACCCGCAACGAAATCAGTTGTTGCTCAGAAGCTTACTAGGAACGATCCCGGTATTGATCAATAAGAGTGGAGGACGTGGGGATGAGTTTACCCATACGAGTGATCAGAAGTGGGATAAAACTGAGACAAATGAGGGCAACCTCCCGGGGTTTGGGGAGGTGAATGGCGGTTTTTACCCAGTTTTACTTATTTAATAGTTAAAAAAGCTTTAGATTTTTATCAAATTTTTATCTATTTAATATTTAAGAAAGTCTCAAATTTTTATCAGTTTATTGGTCAAAAAGTCGCAAATTTTTCTTAGTTTTTTATTTGTTTAATAATTAAAAAAGCTTTAGGTTTTTATCTATTCAATTAATAAAACCTTTACCCCCATCACCGATTCAAAGGCTGATCTGGTTAGTTTGGCACAACTTGATTCTTCCTATCTCATCTCCGACCAAACCCTTTTTAACACCAACTTGTTTGTGTTGTTCAAGTCTAGGGATGTGAAAGTCAAGTATGAGTCAAGTGGCTCAAATACCAACACAATTAGTTTTGATTCAACTAGTCAAGCTAACAAACCATCCTACATCGTTGAGTTTACTAATTCCACCAACATTGGCATCAAGTGAAGCGTGGTGAAAAAGTTTAAGTTAGATGTGCCAAGTGTTTCAACAACCATGAACGAAGTGTTGCAAAACCTCATCCTTGAACAACCTTTGACTAAGTATACGCTTAATAGTAGTTTGGCTAAACAAAAGGGCAAAAGCCAGATAGAGGTGCATCTGGGTAGTGGGCAAGCAAATAATTGACAATCGATGCGTAATCAACACAACCTAAACAACAATCCCTCTCCCAATGCATCAACCGGATTTAAATTAAATAAAGGCAACGCATATAGAAAACTAAGTGAATCCTGACCAATTTACCAACCAATTGATGGGACCAAGCACGGCAAAGGGAAGGATAGTAGTGGGTGGAATAGTGAAGAAAATACGGCAGCAGGGGATGCGCCCAGTGTTACTGGAGGGAGGAGGTGGGGGATCAAATTAATAAATTCACCAAGTACCTCAACACCAAACAAGCATTGGAAAGGATCGGCATCTTGTTTGATGGGGATGGAATGAGGAATGTGGTTAGCCTCCTTCCTCTACTTTCAACCCAACAAGGTGAAAAGTGGTCAATACCAAACCACCAACACCTACAACAAGTTGATTGAGCCTGACAAGTGAGAATCATCAAGTGATTTGAACAATATGACCAACTTGTTAAAGTTACTGACAACTAAAAATGTGAAAGCAAAGTTGGGGAAGACAGCTTCTTCGCAGGGAAATAGTGGAGGGGGGGAAATGATAAAAATGTTTTTAAATAACTACATTAATTCCCTTAAACTTACTAAATTATTTTTCCATTTCGAACTATTATTTGAAAAAAGTTATTAATTGCAAGTTGATAAAGAAAATGAAGCAAAATAACAAGAAAGAATTATTTAAATTTAAATAGACTAGAATTAAATTAAGACCAAAATGTTGCATGCAAACAACATATGCTCTTTTAGTTGTTTTTGGAATTTTATTAGTTCTAATTGGACTAGGATTTTTATTTTGAGCTTTAAGAATTTCACTTAAAGAACAAAAGAAAGGTCAAATTACTAATGATTTGACTTCCAATAACTCACTTAAAAATGAAGTTCCTAGAGGGAGTTGATGAAAAAGAAACAACAAATTAGTTTTATTTTTGCTTGCAATAGTTTTTTTAATGCTAGGAATAGGTGGACTTGTTTCCCTTCCTAAGCTGTTTGGTTAATAAAATTTAGTAGTTTTAAAATGCAGATTAGTTTAGTTAAAATCCGCAATAAGTTTAAACAAAGAAACCGTGGTTCTTTTCGTCAGTGAGTTGGTAAGCTTTCCAACGGTTTGATGATCCCTATTGCAGTTTTGCCTTTAGCAGGTATTTTTTTAGGAATCGGTGATGCCATTTCTTCCAATTCATCTGGCATTGTTGGTGTGAAATTTTTTGGTGAATTTATTAAACAAGGTGGTAATGTAGTTTTTGCTAACTTACCTATTTTGTTTGCAGTTGCAATTGCGATCACCTTTTCTCAAGATGCAGGGGTTGCTGGATTTTCTGCTTTTGTTTTTTGGGCCACAATGAACGCGTTTATGAGTTCATTAATTATTCCTGTTGATGCAAATAATACTGCTTCAGGTTATAACATCCTTTATTGAAAAGCAGTACCTCAGTCAGCAATTGCTTCTACTTTAGGATTAAATTCACTTTCAACTTCAGTTTTTGGTGGGATTATAGTAGGGGCTTTAACTGCATATTTATATAACAAGTTTTATGCAATTAGATTGCCTGATGTAATTGGGTTTTTTAGTGGTACTAGGTTTGTTCCTATTATTTGTATGACTATTGCTATTCCAGTAGCATTACTTTTATTGATGGTTTGACCTGGTGTTTCTATCTTATTAAATTTAATAGGAACTGGGCTTGGAATCTTAGGTGGAAGAGGATATGGTGCTAACAGTTTAATCTTTGGATATATAGAAAGAGCACTAATTCCTTTTGGAGTACATCATGCCTTTTATGCACCATTATGATATACAAGTGCAGGCGGTAGTTTGCAAGAAATTGCAAATCAACAAGTTTGGATTAGAGCTCCTGGTAGTGATTATGTAACCAGAGTGATAGGTTGAGAAGATTTTAATACTCCAGGAAAATGAGTTATTCCTGCTGCTTTAGCTAATGGAACAAGTGGAATGATGAATGGAGCTACTACAACAGGACAAGATAGTACATCTGCACTTTCAAAATACATGAGTAAAGAATCAACAAACTTTCTAAGTTGAAAAGAACTTGTTGATGGTCTTACACGTAAAGGTAACTTTGATGAATTGGCTAAAAACGGTTTATTAGATGGTTCTAACAAGATTTGAATTGGTTTAAACCAGTCAGGGATCTTAGGTAAAAAAGTACTGTTAAGTGATGGTAAGGACTACACTATTACCTTTAAAACTTTTGCTAACACCACACCAACATTCTGAAGCCATGGTGCTCATGCACTTTTACCAATTAGTGGAACTCCAAGTGCAATAACTAATGGAGTTACTGTTAATGGTACTGCTAATTCTAAAACCTATAATGTCAGTCAGTTCACTATTGCAGTTCCTTCTTTAAACCCAGCACAATATTCCCAAGGTAAATTCCCATTCATGCTAATTGGAATTCCAGCAGCTGGACTTGCAATGATCTTAGCTGCTCCTAAGGGTAGAAGAAAAGAAGCTAGTTCTATTATTGGTAGTGCTGCATTCACTAGTTTTCTAACAGGGATCACCGAACCTTTTGAATTTACCTTTCTTTTCTTAGCACCATGGTTATTCTATGGTATCCACGCTGTATTAGCTGCAGTAAGCTTTTGATTAATGAACTTATTGAGTGCTAACGTTGGACAAACCTTCTCAGGTTCTTTCATTGACTTTATCTTGTATGGGGCTTTACCTGATGGTAGGGGTTGATTAGCAAACTCTTACTTAGTACCTATTATTGGTATCTTTTTAGCATTGATTTATTTCCCTACCTTCTATTTCTTGACAATTCGCTTTAACTTAGCAACTCCTGGTAGAGGTGGTAAGTTAATTACTAAAAAAGAATATTTAGCAGCAAAAGCAGCTCAAAAAACTGATCAAACTACTATCACTAACTTTAATCAAACCCAAATTGAAGCTGGTATGTTACTAAGAGCTTATGGTGGAAGTGAAAACATTGCTGAATTAGGGGCTTGCATTACTAAATTAAGAGTAACAGTTAAAAACCCTGAACTTGTTAATGAAACTATTATTAAAGACTTGGGAGCAGCTGGGGTAATGCGTACCACTCCAACATTTTTTGTAGCAGTGTTTGATACTCGAGCTGCTGTTTATAAATCAGCAATGCAAGATATTATCCAAGGCAAAGTAAATTGAACAGAGTTGCAAAAAGTCTTAGATAAAAATGATAGTACTGTTGAAAAACCAGAAATAAAACCAACCCCAGTTTTAAAAGTTCAAGATGAAATTGTGATCCTCTCACCAGTTAATGGCACCTTAAAACCGCTCACCCAAGTTCCTGATGATACCTTCAAAAATCGTTTGGTAGGAGATGGAATTGCTATCTTACCTAGCGATGGGCACTTCAAAGCACCAGGTGATGTGGGTGTGAAAACTGAACTTGTTTTCCCTACTGGTCATGCCTTTATCTTTGATGTTGATGGTGTGAAAGTAATGCTTCACATTGGGATTGATACAGTAAAAATTAATGCTGATAAAAAACCAGGGGAACAACTTGAAGTGTTTGATGTTAAAACAAAACAAGGAGAATACACTAAATTAAAGAGTGAAAGTGTTGTTGAAGTTGATTTAAAGAAACTTAAACGAAAGTATGATCCAATCACTCCTTTCATTGTGATGCAAGAATCACTTGATAACTTCAAGTTGGTGCCAATTCGCCAACGTGGTGAAATTAAAGTTGGCCAACCTTTATTTAAACTAATTTATAAAGATAAGAAGAGTTAATCAATAAAACTCGATAATAACTAAAAGCCATAAAACCTTGGTTTTGTGGTTTTTAGCTTGTTTATAACAATATTATTGCAGTTTTACTGCATAATGTAAAATTACACAGCATGTCAGATACAAATACTGAAAAACCTGAGTTAGTTTCCCTTAATAAGTTAAGTGAGATGCGCACTAACATCGGGATGGTTAAACGTTATTGAAACCCAAAGATGGGATTCTTTATCGAACCTGAACGTAAGCATAATAACGATTTATTGAAGCTTGATCTACAGTACCAAGCGTTAAAAACTGCTTATAACTTCATTAAGGATGTTGTTAAAAATCACGGACAAATCCTTTTTGTTGGAACAAAGAATGATTATGTTAAAAAACTGGTAATTGATATTGCTAAAAGAGTTAATGTTGCATATATTACCCAGCGCTGATTAGGTGGTACTTTAACTAACTTTAAAACCCTTTCTATCTCAATTAACAAACTCAATAAATTAGTTGAACAGCAAAAGCAAAATGCAAATGATCTAACCAAGAAAGAAAACCTGTTACTTTCAAGAGAGATTGAAAGACTTGAAAAGTTCTTTGGTGGGGTCAAAAATTTAAAATGACTTCCTAATCTAATAGTTATAGATGATCCTGTTTATGAAAAAAATGCAGTTTTAGAAGCAAACAGCTTAAAAATCCCTGTTGTGGCACTATGTAACACCAACACCAATCCAGAGCTAGTTGACTTTATTATTCCAGCTAATAACCACCAACCCCAAAGTACTTGTTTATTGATGAATTTACTAGCAGATGCGATAGCAGAAGCGAAGGGCTTTGAAACCTTGTATGCTTACAAACCAGATGAACAGATCCAAATTGAAATTCCTCCCAAACAAGAACGCCAAGTTATTAACCGCTCCAATACCAGAAACATCACTAACCAGCGCTTAAACATTAACCGTCAGCAACAAGAAACTTTATAGAGCAGTGAACAGTTGAACAGGACTTAGTGAACAAGCGGTAATTAAAAGTCGTCAAGAACATGGTGCTAATTTTCTTCCTGAGAAAAAAGCTACCCCTTTTTGGTTGTTATTTCTTCAACAATTTAAAAGTTTAGTTGTTATTCTTTTACTGCTAGCTAGCTTGTTATCGTTTGTAGTTGCTATTGTCAGTGGTTTGAGAAGTAACTGAAACTTTAACCACAATCTGATTATTGAATGGGTTCAACCTTTTATTATCTTATTAACTGTTTTTGCCAATTCACTAATTGGTTTTATCCAGGAATTTAAAGCCCAGAAATCTGCTAGTGCTTTAAAGTCCTTGACAAAGTCTTTCACAAGGGTTTTTAGGAATGGTGAATTAATTAGCATTAATGTTAGTGAAGTTGTTGTAGGAGATATTATTTTTGTTGATGCAGGGGATATTATCCCTGCTGATGGCAAATTACTACAGGTTAATAACTTACGTTGTTTGGAAAGCTTTTTAACTGGTGAATCAACTCCAGTTGATAAGACTATTGATAGCAATGAAAAAGCTAGTATCCTTGAACAGACAAACTTAGTTTTTTCAGGGGCACAAGTAGTTTATGGTAGTGGCGTTTTTCAAGTGGAAGCAGTTGGGATTAAAACCCAAGTTGGACAAATTGCTAAAACTGTTGATGATAGTGTAACCAAGTTATCACCCTTACAACAAAAACTAGAGAAGATAGGAAAGTGATTTAGTTGATTTGGGCTTGGTCTTTTTGCTGTAGTTTTTCTTGTCCAAACTGCTTTATTAGGATTTGATAATTTCACTAATAACTGATCAATAGCTTTAATTGGTGCTATTGCGCTTGTTGTTGCAATTATCCCTGAAGGGCTTGTTACTTTTATTAATGTGATCTTTGCATTAAGTGTGCAGAAACTAACTAAGCAAAAAGCCATTATTAAGTATTTATCAGTAATTGAAACACTTGGATCAGTACAAATTATCTGTACTGATAAAACTGGTACTTTAACCCAAAACCAGATGAAAGTTGTCGATCACTTCTGTTTTAATTCAACAACCCAAACTGATCTAGCAAGAGCATTGTGTTTGTGTAATAATGCTTCTATTTCCAAAGATGCTAATAAAACAGGTGATCCTACTGAAATTGCTCTCTTGGAATGAAAAGATCGCAGTCAATTAGATTTAAAAACCTATTACAGGGTTTATGAAAAAGCCTTTGATTCAATTAGAAAACTCATGACAGTTGTTGTTCAAAAAGACAACCGCTTCATTGTGATTGTTAAAGGTGCTCCTGATGTGTTATTACCATTATGTAATAACGTTCAAAATGAAGTAAAGAACATTGAAAACTTACTTGATCAAAGTGCTGGTCAAGGCTTGCGTACCTTAGCAGTTGCTTTAAAGGTTTTATATAAGTTTGATCAAAACGATCAGAAGCAAATTGATGAACTTGAAAACAACCTTGAATTCCTTGGGTTTGTTAGTTTACAAGACCCACCAAGAAAAGAAAGTAAGGAAGCGATTTTAGCGTGCAAAAAAGCTAATATAACCCCAATAATGATTACAGGGGATCATCTTAAAACTGCAACTGCAATTGCTAAAGAGTTAGGCATTTTAACTTTAGATAATCAAGCAGTTTTAGGTAGTGAACTAGATGAAAAGAAGATCTTGGATTACAGGGTATTTGCTAGAGTAACTCCTCAACAAAAATTAGCCATTGTTAGTGCTTGAAAAGAAGCGGGATTTACAGTTAGTGTTACTGGTGATGGGGTGAATGATGCACCTGCATTAATCAAGAGTGATGTAGGGTGTTGTATGGGGATTACTGGGGTTGATATTGCAAAAGATGCTAGTGATCTGATTATTAGTGATGATAATTTCGCTACTATAGTAAATGGTATTGAACAGGGTAGAAAAACCTTTTTAACTTGTAAACGAGTTTTATTAAACCTGTTTTTAACTTCAATTGCAGGAACAGTTGTAGTTTTATTAGGACTATTCATCTTAGGACAAGTTTTTAAAACTAATTTATTACAACAAGGTCATGACTTTCAGGTGTTTAGTCCTACCCAACTGCTAATTATTAACTTGTTTGTTCATGGTTTTCCTGCTGTTGCATTAGCAGTACAACCTGTTAAAGAAAAACTGATGGTAGGTAGTTTTTCTACTAAAAATTTGTTTTACAATCGCCAGGGCTTTGATTTAATCTGACAATCACTATTTTTAAGCTTTTTAACTTTACTGTTTTATAGCTTAGGAATTATATATGCAATTAATAACCGTGATTTACAAACTAGCGGGGATCTAATTAATCGTGCTGGATCAACGTGCGGTTTTTTATTTTGGGTGCTAGTGCTGCTTTAAACTCATTAAACCTAATGGTAGATAAACCATTGCTTATGACAAACCCTTGGTTTTTTAAGTTAGTTTGAATAGGTTCACTTGCTTCTATACTCGTATTTTTATTGATCATCTTTATCAACCCTTTAGGGTTAGTGTTTAATGTCTTGCAAGATTTAACTAATCACCCAGTTTTAATAGGCTATAGTTTTGGGGGAGTTATTTTGTATATGGGGATGAATGAAGTTGTTAAACTTATTAGATTAGGTTATGGCAATATTTAACTTCCTTAAGTTAATTTCACCCAAAAACAGAATTCTCAGTAAGGCAAATAGGATTGCCAGTGAGGTTGAGAGTTATAAAAACTACTACCGTAACTTAACTGATCAACAGTTATTTGAAGAGTCAAATAAACTAGTTGATCTTGTCACTAAGCAAAATTACACCATTCTAGATGTTTGTGTTGCTGCACTTGCTTTAATTAGAGAAGTGGTTTACCGTGAGACTGGTGAATTTGCATATAGGGTGCAGATCATAGGAGCTTTTATTGTTTTAAGTGGTGATTTTGCTGAGATGATGACTGGTGAAGGTAAGACCTTAACCATTGTTTTAGCAGCATACGTTTCTGCACTTGAAAAGCGTGGTGTGCATGTTGTTACTGTCAATGAATATCTAGCTCAAAGGGATGCTAATAATGCAATGAAGATCTTAAAACGGGTTGGGATGAGTGTTGGTTGTAACTTTGCTAATCTCTCCCCTCAGCTAAAACAAGCTGCATTTAATTGCGATGTTACCTACACCACTAACAGTGAACTGGGGTTTGATTATCTTAGAGATAACATGGTCCACAGTTATCAAGATAAGAAGATCAGAGAGTTGCACTTTGCAATAGTTGATGAAGGTGATTCAGTTTTAATTGATGAGGCGCGAACGCCTTTAATTATTTCAGGTCCTAGTAAAAATGAGTTTGGGTTATATGTTGCAGTTGATCGATTTGTTAAATCATTAACTGAACAGGAGTTTAAGATTGACCCTGAATCACGTGCTGCTTCTTTAACTGAACTTGGGATTAAAAAAGCAGAGCAAACATTTAAAAAGAAAACCTTTTTGCTTTGGAAAACAGTGATCTTTTTCACAAGATCATGAATGGTTTGACTGCTGTGAAAGTTTTTGAACAGGGCAAAGAGTACATTGTTCGTGATGGCAAGGTTTTAATTGTTGATCACTTTACAGGTAGGATATTGGAAGGGAGAAGTTACAGTAATGGCTTACAACAAGCTGTACAAGCCAAAGAATATGTTGAGATAGAACCTGAAAATGTGATAGTAGCTACCATTACCTACCAATCCTTCTTTAGGCTATACAACCGCTTAGCAGCAGTATCAGGTACTGCTTTAACTGAATCAGAGGAGTTTCTCAAGATTTATAACATGGTTGTAGTACCAGTGCCAACTAACCGTCCTAACATCAGAAAAGACCGTTCTGATAGTGTATTTGGTACCCCACAAATTAAGTGAATGGCAGTTGTTAAAGAGATAAAAAAGATCCATGAAACTTCTCGACCTATTCTGATTGGAACTGCTAACATAGATGATTCTGAACTCTTACATAATCTGTTACTAGAAGCTAATATTCCCCATGAGGTTTTAAATGCTAAAAACCATTCAAGAGAAGCGGAGATAGTAGCTAAAGCAGGACAGAAGAATGCAGTGACTATTTCAACTAACATGGCTGGAAGAGGAACTGATATCCGTTTAGGTGAAGGGGTTGCTGAAATGGGTGGTCTTTATGTATTGGGAACTGAAAGAAATGAGTCAAGAAGGATTGATAACCAACTAAGAGGGAGAGCTGCTAGACAAGGTGATAAAGGGGAAACTAAGTTCTTTATCTCACTAGGTGATTCATTGTTTAAACGTTTTGCTCATGACAAGATTGAAAGAGCGATTAGCAAATTAGGTAATGAAACATTTGACAGTGCCTTCTTTTCCAAAATGTTAAGTAGAACCCAAAAACGGGTGGAAGCAATTAACTTTGACACTAGAAAAAACCTGATTGATTATGACCATGTTCTTGCAAGTCAAAGGGAATTGATTTACAAACAACGTGATAAGTTTTTATTAGCAAACGATTTAAGTGAAATGATCGACAAAATGCTAGAAAAGTTTGTACAACAGTTTTGTGATCAATATAGAAACCAAAAGAACCAAAACTTAATTAATCACATTGCACTAGCAGAAGCTTTAAATCTTGAGATGAACATGCAAAACACCATTAATCCAAAGGTGTTTGAAAACATGACTTTTGATGTTGCTGTTGATAAAACCCGTAACTTAGTAGCTAAAAAGATTAGTGATAAAGTTAATGTTTTGACCAAACCAATTGCTTTAAACAGGTTTCGTGACATTATCATAACTTCGATGGATAAACATTGAACTGAACACTTGGATAGTGTTTTTAAGTTAAGAGAAGGGGTTGTACTTCGTTCTATGGAACATACAAGTCCTTTAAATGTTTACATTAAAGAAACAGATATCCTTTTTAAAACAATGTTGCAAAAGATTGCTCAAGATGTCATTGTGCAAATTGCTAACCTCACAACTCCAGATGAATTTGATCATAGCTTAATGCAAGCCAATGCTTTAAAGAAACTAGCAGCAATTAAAGCAGATGAAAAATCAAACCAAGAGTAATAGTTTATTTCAACTTTCCACTAACTATATACCTACTGGTGATCAACCTGAAGCAATTAAGAAATTATCAGAATTTAAAACTAAGCAGCAGGTTTTATTGGGGGCCACAGGCACAGGTAAAACCTTTACAATTGCTAATGTAATTCAAAACAGCCAACTCCCAACAGTTGTTATTGCTCATAACAAAACCCTAGCAGGTCAACTCTTCAATGAATTAAAGCAACTGTTTCCTAAAAATGCAGTTGAATATTTTATCTCTTACTTTGATTTTTATCAACCTGAAGCTTACTTACCCAGTAAAGGGATCTACATTGAAAAAAGTGCTACAGTCAATGAAGCGATTAAACGCTTAAGAGTCTCAACACTGCATTCACTTTCAACAAGAAAAGATGTTATTGTAGTAGGTTCTGTTGCTAGTATTTATCCCACCTCATCTCCCAGTGATTTTGTTAAGTATTGCTTGTGGTTTGTGGTTGGCAAAGATTATGATTTGAAAACCATTAAAGATAGGTTAGTTAGTCTTAACTATGTTGTTAATAAACAACAATTAACCCCAGGAAAATTTCGCTTTCAGGGTGATGTTTTGGAGGTATTTCCTGGTTACAGTGATGCTTTTGTGATCAGAATCTCCTTTTTTGATACTAAAGTAGAACAAATTTGTCAAATTGACCCACTAACAAATAAGATTTTAAACCAACTCTTTGAGATTAAGATAGGTCCTGCTGATGAATATGTTGTAAACCAATCTGATCTTGATATAGCAATTAAAAATATTAAACAAGAACTTCAGGAACGAGTTAATTATTTCAATAAGCAAAATCTTGTTGAAAGAGCACAACGTTTAGCCACCATTACTAACCATGATCTCAATGATCTGAAGGCTTGGGGATTTTGTAGTGGAGTTGAAAACTATGCTAGACACTTAGAGTTGAGGATGGCTAACTCAACCCCTTACAGTATCTTTGATTATTTTAAGGGGGATTGGTTATTGGTTATTGATGAATCACACCAAACTTTACCGCAACTTAATGGGATGTATAACACTGATCTTTCAAGAAAACAAAGCTTAATTGATTATGGTTTTCGACTCCCCTCTGCACTTGATAACAGACCGCTCTCATTTGCTGAATTACAACAAAAAATGCAAAAAGTTATTTATGTTTCAGCAACTCCAAGAGATAAAGAGATTAGTTTAAGTCAGAATAATGTCATTGAACAGTTAGTTAGACCAACCTACTTGGTTGATCCTATTATCGTTGTTAAACCAAAAGATAACCAGGTGGAGGATCTCATTGAAGAGATTATCAACCAACGCCAAAACAACACAAGAACATTTGTTACTGTTTTAACTATTAAGATGGCTGAAAACCTCACTGAATACTTAAAGGAACGCAAAATTAAAGTTGCCTATATCCATAAGGACATTAAAGCATTGGAACGTTTATTGTTAATTAATGACCTGAGAAGAGGTGAATATGAGTGTTTAGTTGGGATTAACCTTTTAAGAGAAGGGTTAGATGTCCCTGAAGTTGCTTTAGTTTGTATCTTTGATGCAGATATCCCAGGACTACCTAGGGATGAGAGAAGTTTAATCCAGATTATTGGACGTGCTGCTAGAAATGAACATGGTCGAGTTGTTATGTATGCTAACCATGTTACTGAACAGATGCAAAAAGCCATTGATGAAACCAAAAGAAGAAGAACTGTTCAAATGGAATATAACAAGCTACATAATAAGATACCAAAAACAGTTGTTAAACCCCTTAGCTTTGTTCAACCAATCAAATTAAAAGCTAAGAGTAATGCAGAAAAAAATGCTGCATTAATCAAACAATTAACCAAAGAAATGAAGAAAGCAGCAGCTAATCAAAATTATGAACTTGCCATTGAGATTAGAGATTCCATATTTGAATTGGAAAAAGAAATTGATAGTAAAATTAAAGTATAGCAATGCGACAGTTTATTAAGCTTAGCCTCTTAGTCTTTGTCTTGCTTTTCTTAAGTGAACTTATCTGTAGGTTCTCTTTACGCTTAGTAAATTCTATTAAAGCGAGATATAAATTTTCAGTATTTTCCTACACTGCTTGTTTGCTATTTTTAAAGAGTTTTCAAAACTTTTCTAATGCTTTTCAGAAGTTAGCTAACTGGGTATTTTGATTTGAAAATGATGTTAATGAACTGTTATCTATCTTTTACTTTAACTTTGATCAGAAGAGTGAAAAAGTAGATTACAACTTCTTTAATGGTTATAAAGTTACTGCTCAAAAAGTAGTTGAAAAAGAACAGTTATTAACTTGTAAGTTAAGTGATTACTACCGTTTATTTAGAGATAAAACCTTTTGATTTGAACTTATCAACAACTAATAAGATCCTTATTGGCTGTTTAAGTTTGCTCTCTTTATAAATTCATAACTTTTCTCTTAACATATGAAATTAAGTACAATAACTACTATCTGCTTGTCAATATCTGGTGCTTTTGGTACTACTGCAATTGCTTTACCAACTACAGTAGCATTGTTAAAAAACCACCAACAGCAAAACACTGAAAAACAACAAAACCCGATTAAAGATATCCGCTTTGGTTTAAACAATGTACAGGTTCCAAATACCATTCCGTTACACCAAACTGTGGTTGAAGTGATCAACAATAAAGAAATTGTTGATTACAAAGATGCACCCCAAAAACTCTTTTTAGCTAAAAGTGCGTTAAATAATAAACTCCAAGTTGAGTTTGATAAGTTCTTGCTAAGAACAGGAGTAATTAATGCTTTAAATGCAGATTTGAAAGAATGGATAGACCAAACATTATTTATCCCCAATCAAAGTTTTTTTGATCTTAGTGCTAATAAACTCAATTTAACTTTGTCAAATCAAGGCGAAGTGAGTTTAGATCTTGATTTTACTTTTACTAACTTTAGTGATAAAAATCAACCATTAAAACTCCCCTTTGACGGTAATGTGGTGGTTAATGCTAACGAATCATACACCTATTCAGTTAAAGCAACACTACAGAAACTAAAAGTATTGACTTTTTCAAGAGCAGATCATTATGTAGGGATTAGTTATGCAATACCAAAAGTGAGTTTAAATGGTAAAACTCAAAATGACTTTAGTTTTAACCCTTTTAAAAGTAATATTAACTTTACTTTTAAAAATGTCTACAATGCTTTAGATCCGTTTGAAGCACAAAAATACCTGGTTGGCCAAGGCAAGTTTTTAAACCAAAAAGTTAATGCTGATGATGTTAAAAATGACATTAATAACCACATTGAAACCCAGTTTAATGTTGCTAAAATCACAGCAACTCTTTTAGGAAAAGCTTTTAAGCAGTTTGGTGAACATAAAAATGGTCAACCACTTTCACTTTTAAAAGTACTAAGTGGATTAAACAATGAGTTTAAACAACTCTTTAATTATGTCAGACCTGGTTTAGGTGATTTTGTTAGTGATTTAATCCAAAGCTCCAGTCAATCAAGTAATAAAAAAACTGTTTACCAGCTATTATTTGAAAACAAGACAACAATTATCCACTTGTTGCAAGATCTTAATATTAGTGAACTGAACTCTGTTTTACCAGTTGTTGATATTTTGTTTGAAGGGATTAACAGTGCAGAATCACTATACCAAAGGATCCAATCATTTAAGGATTTAATTGTTCCTGCTTTAAAAGCTGATAAGCAACTTAAGAGCTTAGAAGCGATTATTTTAGCAGTTTTAGACAATCCTAATACCTATGTTTTTGATCTTGTTTACCAAAACAAATCGATTCTGTTTAATTTGTTAAGTGATTTTCTTAAAAACACCGCAAACACTTTACCATTTCTTCAAGAACAGTTTGATATTGTTAACCACTTATTTGCCAATGAAGCGATCTTTGATCTGTTTTCCAATGCTGACTTTGTTGATAAGATTGCTGATCTTTTTTTAGCAAAACAAAAGGTACAAGAAGTTAATAATGATGGAACTAAATCAACAAAAATTGTTGATTCGATTTTAGTTGCTACCCTTAAGGGTTTAGTTGGTAATCAGCTCAGTTCAATCACTGAACTGTTAAACATCTATATCTTTGAAAATGAATTTTTAAACAGAAATGATAGTAATAGTTCAGTTAAAAAACAACAAACTGATAGTCTAAAAAATCTGTTTAGCGTAATTGGTGATATTTTAAGTGAGACCAATGTTAACAAGATCACTTTACACGCAGTTAAAAATAATGAACTTCTTTCGTTAGTTGAAACAGCTTCAACACTAAAAATTAAACATCTTAATGTTCAATACAAAGTTTTAGTTGATAAGTTTGAATTGAAAAACAGTTTCATTAAGGCACTATTAAATTTTTTCCCAGATACTAAAGATATCACCCCAACTATTAAAAAGGTACTGTTTGAAAGTGAAAATTATAAAACACTCCGTAAGAAGTATGAAAATGAAGGGTTCCCAGGTTATCACTGGGCTAAGTTCATTGTCCCAGGTACCTTCAATTCTGCTGAAAACACTTTCTATTCTGCTATTGATAAAACTAAATCAATTCGCGATCTGTTTGCTGACATGTTATTTGGTAAGAGTTTAGAGAGTGTTAATGACAGTGATAGTTTTATCAAAATTAATGGTTCATTTACATTAAAGTACCACGGTGATAACTTAAATTTACTCCCTAACTACCACTCATTAATCACTAAGAATGTTGGTTATCAAATAGTTAATGTTAATTTTCATATTGATGCAAGATTGCTAACTGCAGAACTACAAAACACGGTATTTTCAAATCCAAAACCAGTTATTAAATCACCTGTTGAACTCTCCAAATCCTTATTTGAAGTTTGAAAGACTATCTTTGAAAACTCAGTTAACCAAATCTTAAAAAAAGAATATACTTTCAAAGATAATCTAAAGTTCTTTCCTTTTAAAGCTGATGGCTCCTCACGCTTAGAATTTGATCTATCAAAACCTGATCAACGTGTTATTCCTTTTGCTTTTGTTGATGGTTATCAATTCCAACTAAAAAAAGAGTTAATTCCTAACAAAGAAACAAAAAAGAAGCTAATTCTTCACCAGTTTTAAAGCTTTATGATGCAGTAAGAAACGATAGACAATATCGTGCTAACCACCACCATGATGATCTGAGAAACTATCCTAGCCTTAAATCTCAACTAGAACTTATATTAAACCTTGGTGATAAGCTTAAAGCTAATAATGACTTTATAGATGACACTGTTGTCAATGCTTTGCAATATAAAACTAGTTTTAAATCTACTCTAAAAGTTAACAGTTTAGGAATTCCTATTAACCTTTTCTTTTTCACATTGTGACTTAAATTTAACTTAGAAATTCCAATTGATGGTTCATTAACATTAACATCTGTTAATGTTGTTTTCCCATACAGTTTGTATGATACTAGTTCAAATGAATTTACAAGGATAGTCGATCGCTTAAATTTCACTGATACCAACTTCTATCTAAAAGATGCATTTCCTAATTTCTGGTTTGTTGGTTTCTAGAGTAATGAAACTAAACCACTAACTAAACTACCAACTAGATAAACAATCAAGTTCATCCACCAAAACCCACTAGTTTTTTGTCTGTAAGTTGCACTTTGATCTTGATATTGATTAAAACTAACTAATGGTTTTGCATAAACACTACCTAACTTAAGATATCTGTTAAACTTACTAATTTCATACCGCATTCCATCACAAGTTTTTAAACGGAATATCCCCTGTAAACAAACAAGTAATAAGTTAACAAAAAAGCTAGTTAGTACTGAATTTTTAACAACCACTGTAAAATTGGTTGACTTGTGAAAATAAATAGTAATGTTTAAGATCAAAAAGAAAAGGATTAGATAAAATACGCTAATAACTATCCCACCATACTCAGCTTTATTACTGTTTTTGTTTTGATTTAAAACCATCTAGTAATCGTTATTAGTATCATTTTTTTATAAAATAATCAAGAACATCAGCACATGATCTTTATCACTAAATTTATGTTTGGTTTCAAATGAAGTTGGATTATAAATCTTGCTTTATTGTAATCATGCTTTAGATTTAGAAGCTGGTTACTTTTGTTTCAGGTAACCAGTACCACTTCCAAAAAACTGAGAACCTGAAAAAACAGGTTCTTTTTTATTTCTATTTAGTAAATGTTCAAGTATATTCTTAAACGATTAGGACTAGCAGTAGTTGCGATGTTTATCGTAATGTCTATAGTCTTCTTTTTAGTGAACGCTACTGGTAATGTTCCCTTGTCAGCCACTTCTGCAAGAGATATTGCTGCAGTGCAAGCACAACTACAAGAGTTTGGGTTTAATGACCCTATTATAATTAGGTATTTTCGCTATTGAGCTAAGCTATTTTCCTTTCAAGCTGATGCTTTAGGAATTTATTATGCAAACCCTAACCAAACAATTGGTGAGATTGTGTTTGCAAGAGTACCAAATACCTTATATGTGGTTTTAATCTCTTTTTTAATTGGTTCATTGCTAGGGATCTTTTTAGGGATGGTTTCAGGATTGAATAGAGGGAAGTTTTTAGATGCAGCAATTAATGTGTTGGTAGTTTTATTTGTATCTATTCCTTCATTTGTAGTGGGATTAGGGTTACTTAAACTAGCAGGATTTTTAAATCTACCACCACGGTTTATTAACTTTGATGATGCTTTTTTTAGCTTTGATCGTTTCTTGCTTGCATCAATTATCCCGATCCTTTCATTGGTCTTCTATTCATCAGCTGCTTTTACATACAGGATTAGAAATGAGGTGGTGGAAGTGATGAATCAAGACTATATTAAAACTGCAAAAAGTAAGGGACTTGGGATGTTTGCTGTAGCTAGGTATCATATCTTTAGAAACTCGATTATTCCTTCTATTCCCTTGTTTGTATTTGGAATCTCAGGTGCTTTTTCAGGTGGATTTATTATTGAGTCTTTGTTTGGAGTACAAGGGGTATCTAGGATCTTAATTGATTCAGTGCAAGTTAATGAAACTAACATGGTAATGTTTAATATCTTGTTTATCCAAGGGATTCCCTTATTAGCAAGTGTCTTTATTGAATTTATCTATGTTTTAGTTGATCCTAGAATTAGGATTGCAAATAGTTCTAATGTTAGCTTATTAACTAAGTTAAAGTTCTTAAGTTCAAGACACCAATGGTTAATGAAGTGAAACAAGATTAACAGTGATAATGCCCAAAATATTGTGTTTAACTCGCCACTGCACCACCAGTTATTAGAACTCAATGCAATTGATTACAAAACAAAAACAGTCCAACTAACAACTGAACAAAAAACTGCTCTCAATATCAGTGCAACTGCTAACTTTATCTTACTTGGTAACAAGTGTTTAAAACTCAAAACAATCCATGGATAGAAATAAAAGTTTTGACCCTAACTTATTTAAAAGGGTTGATATCAACTTATTAAAGCGAAATGATCAGCTTATTGGTAAACCAACTACCAATTCAATAGAAATTATCAAGCGCTTGTTTCAAAACAAGTGGGCCATCTTATTTTTTCTTTTAATAGTTGTTATTGTGCTATTAGCAATTATTGTGCCTTTAACTTCCCCTTTTTCAGCAGTAACTCCTGTTTCAACCAATGCCTTAGCACAAAATCTACCACCACGGTACTTATGGCATAAACCAGGTGACATTTTAGTTCATAAGATTACAGCAAGATCAATTGCTGAAATCTCTCAAGCTAGTGGAGTTTTAGTAGGAACATTACCTAGTGCAAATAGTAATCCCTTAGCAACTAATGTCCAGTATGATATTGCTCCTTTTCAACTCCAAGAATTGCGTAATTATTTCCCTTTATTGGGGACTAATGGACTTGGGATTGATATTTGAACCTTGTTGTGAGCTTCTGTTGCCAAGTCATTGTGAATTGCAGTAGTAGTAGCAATTATAGCAATGGTGTTTGGAACCATTTATGGAGCGGTTGCTGGAAGCTTTGTTGGACATATGGCTGATAACATTATGAGTAGGATCATTGAGATTATTGATATAGTCCCTTCTATTCTTTGAATTATTGTTTTAGGAGCTACATTCCGCTTTGGTGGGGTTAAACAATTTGATGATAGTGTTGTAATCTTTACTTTAATCTTTGTGTTTTGAACATGACCTGCTACTACAACCAGAATTTACATTTTGAAAAACAAAGATACAGAGTACATCCAAGCAGCTAAGACCTTAGGGGCACACCAAATCAGAATTATCTTTGTTCATATGTTACCTGTTGTATTTGGGAGATTAGCTGTTGTGTTTGTTAGTTTAATCCCAGCAGTTATTGGTTATGAAGCTTCCTTAGTTTTCCTTGGGTTAAAACCAGCTACTGATATTGGCTTAGGGGCACTTTTAAACCAAATAACTTCAAGTGATAATGTAGCTTTAATCTTAAGTTCGATTGTTAGCTTTGCAGTTTTAACAGTAGCAGCTAGAACATTTGCTAATGCTTTAAATGATGCAATTGACCCTAGGGTTGTAAAACGATAAAATGGCACTTAAAAGAAGTAATTTCTTTGTTGATAAAGACCAACAACTAAAGGATAATTTGATCTTAGACATCACTGATTTACATGTTAACTTTAAGGTTAAAGATGGGATCTTACATGCTGTTAGAGGGATTGATCTTAAGGTAGAGAGGGGTAGTATTGTAGGGATTGTAGGTGAATCGGGCAGTGGTAAATCAGTGAGTGTTAAATCAATTATTGGTTTTAATGACAATGCACAAACTAAAGCCAAACTGATGAACTTTAAAAACGTTGATATTACCAAACTAAAGAAACACCAGTGGAAGTATTATAGAGGGACATATGTCTCTTATATTTCCCAAGACCCATTGTTTTCTCTAAACCCAACAATGACGATAGGAAAACAAGTAAAAGAAGCGATTTATGTGGCTTCAAAAAGAAGGTATTTCCAAGCTAAATCAGACTTAAAATTTGCTTTATCAAATAAGGAGATTGACAAAAAAACTTATAAAAGTAAACTAAAAGAGATCAAACAAACCTACCAACAAAAAATAAAACCTATCAATGTAGAGAAAAAAACCTTAGAGATCCTGCAGTTCATTGGTATTAATGATGCCAAGAAACGTTTAAAGGCATTCCCAAGTGAGTTTTCAGGAGGGATGAGACAGAGAATTGTGATTGCTATTGCAGTAGCAACTGAACCTGATTTAATTATTGCTGATGAACCTACTACTGCACTTGATGTAACTATTCAAGCTAAGGTATTAACTTTAATTAAACAACTCCGTGATCTACTTAATATCACTATTATCTTTATTAGTCACAATATCTCTTTAATTGCTAATTTCTGTGACTTTGTTTATGTTATGTATGCAGGAAAAATTGTAGAACAGGGTCTGGTTGAAGAGATCTTTACAAATCCACTCCATCCCTATACATGAGCATTGATTTCTTCAATTCCTGAACAGAAAGATAAAAACAAGCCACTAACTTCTATCCCTGGAGTTATTCCTAACATGTTAACCCCACCAAAGGGTGATGCTTTCGCTAGCAGAAACCAATATGCTCTAGCAATTGACTTTGAATACCATCCACCCTTTTTTGAAGTTACTAAAACCCATAAAGCAGCAACCTGATTGCTGCATCCCCAAGCCCCTAAAGTTGAACCACCCCAAGCGGTTATTGATAACATTACCTTAACCAAAAAAGCACTGCAATTTAAAGATCAATAATGGAAAACCAAAACACAAAAAAACCACTTGTTAATGTTAAGGCTTTGAGCATGATGTTCAAGGTCAGAGGAACTCTTTTTAAAGCCCTTGATGAAATTGGTTTTACTGTTAATGAAGGGGACTTCTTTGGGGTTATTGGTGAGAGTGGTAGTGGTAAATCAACCACGGGAAAATGTTTGATTAGATTAAACATTCCTAGTGGTGGAAAGATTGAGATTGCCAACCACTTACTCTCAGGAAAAAAACTTACTAAAGAGAATAACCAGTGGTTAAAACAAAACATCCAAATGGTGTTTCAAGACCCTTATTCATCTATTAACCCTACTAAAAATGTGCTAACTGTGATTTCAGAACCGCTGGTAATTAGTAAAACTGTTTTTGGGGAAACAAAACAATACTTAAAGAGTTTGCAAAAGCTCTCTTTTAAAGTAAAGAAAACATTGTTAAGGAATGATATTGAACTTGAAACCAAGTTTCACAATAACTTTTTTAAAACCGTTATTAAGCAAATTAATGAATCATTGTTTAACTTTGAAGATCTTGATTACAAGGATTTAAAACCATCACATTTAAGGCAAAGAATCATAAATGAAACAGATAAATTCATTGAAAAAATTAGAAGTGAGTTTGCCCTTTTTTATGATTTTTATGCTAACCAATCAGTACCCTTGCAAAAGGCATTAGATGATGCGAATTCCTCTTTAACACCATCTAGTGTTATTGAGTTAAAAAACCAGTTAAAAGCATTACAAAAACAAGCAAAGATTTCAAAGGCAGCATGGGATATTTTACAAGCCCTAAAGCAAAACCAAAAGGAGTTGAAAGATTATGAAAACTATGTCCATTTTGAACTCCAAAAAAAGCCACGAATCTATCTTAATACCTGGCTTTTAACAACCAAAAGCTACATTAAAGATTCCAAGCAAAACATGCAGCTTACTGATGATATCTTTGCTTTTTCATACAACAGTATGGTTGACAAGAAAAGAAACTTGGTTTTAATTCTTTCTAAATACTATAAGCTGTTACCTTATTTCTATGACCAATCAGTATTTGATAATGCTGATCAATTTGATGAAATTGCTAACCTTATCTTTTTTGATTTAGTTGAAACATTGCTTGGTGTAACTAGTTTATTTAATGATGCATTAGCAGCTGATAAAGTCCCACTAATTAAGTTTGCTAAGTTCTTAAATAAGTTATGTGACTTGCGCTTTTTAACCTTAAAAAAGAGCTTTAAAAAAACAAGAGTAAGTTGTAGCTTTAGTTTTAACAGTGAACCTGAAATCTTGTTTGCCAACAGCTGCTATGATTTGCAACAAATGCCTCAAATCATTAAACCCTTTTGAGAGAAGCTTTTTAATGAACAGAACTACCAAAAGATTATTGATTCAGTTTCAAGACTGAATGTAATGATTGCAAATGACATTACCAAAGCTTTTGAAATTAAAAAAACTATTGATGAAAAACTAAGGGAGTTTAAACAACAAAATTTAGCTTTAAAAAAAGCTTATTCAGCTAACAAGAAAAGTGAGGCAAACAAAGCTTCCATTAATGAGTTAAAAGTCAATTTAAAAACACTTAAAAAACAGCTTAAACAAGAGAAAAATACTACTAAAAAACAATCAAAAAAGGAATTAAAACCACTTTTAAAAGAACACCATACTGCTTTAAAACTCCATGATGAGTTTAACCATGATTTACGCAAGTGGTTCAAAAAACTTAACTTTATGGTTAAGAAATACAACCGACTGGAAAACAGCCAGAAAAAGTTTTGTTTAGTTAAAAAGTTAAAAGCGCTTTTCAAAAAACAGGATGAAACACTACAAAGTGAATTAAGACCAAAACTAAAAACATTTGGTGTAATTAACTTTGAGTACAAACGTGCAGTCAAAGAGTCCAATGTCTTTCGATTGGTGCATTTTGCTAAAAATATCTTTAAACCATTCTTGTTTTTTAACCTCACCAAGATTTTTATGAGAAATAAGGTCTATGAAGCACTCGATAGTGTTGGTTTAAAAAGAGAACATGCTTACAGATACCCCCATGAATTTTCAGGCGGACAAAGACAAAGAATTGCTATTGCCCGTGCTTTAATCACTAAACCCAAACTGATTATTGCAGATGAATTGATTAGTGCACTTGATGTTTCTATCCAAGCCCAAGTTATTAACATCTTGAAAGACTTGGCTAAAAAACACAACTTAACTGTGCTTTTCATTGCCCATGATTTATCAATGGTGCAAACTGTTTGTAACCGTTTGATCATTATGCATAGGGGCAAGATTGTTGAACGGGGCAGTGTGGATGAGATCTTTTCAAATCCAGTTCATCCCTACACCCGTTCCCTAATAAAAGCATCTCCTAAGTTAAGCAAAATCAATGTTGATCTCGCTTCTTTTGATGAAAACTTCACTTATGATAGTGATTATTCACTAACCAATATGCCCTTTTATATTAAAGTTCCTAACAGTGAAGAACATGAACTTTACTGTACTCAAAAGCAATTTGATAGTTGAATCAAAGAGGCTACGCCGATAAATTAATTTAGAATTTTCCAAAAATGTGGGAGCTAATTAAGCGTTTGTACCACAGCAAAATATGGCTAAAAAAACAGTTACAAGAATCGCTAAGATTAACCTAATTGGCGGACAAGCAAAACCTGGCCCTGCGCTTGCTTCTGTAGGGATTAATATGGGTGAGTTCACCAAACAATTTAATGAAAAAACCAAGGATAGACAAGGTGAAACGATCCCTTGTATAATCACTGCTTTTAACGATAAATCATTTACTTTTGTCTTAAAAACTACCCCTGTTAGCAACTTAATTAAACAGGCTGCTAAACTAGAAAAAGGTGCTAAAAATGCAAAAACTATTGTTGGAAAAATCTCCTTACAACAAGCTAAGGAGATTGCGCAATACAAGTTAGTTGATCTTAATGCTAACACAGTTGAAGCAGCATTAAAAATGGTGTTAGGTACAGCTAAACAGATGGGAATAGAGGTAACTGATTAATGAAAAAACTATCAAAAAAGATGCAAGCTGTTACTAAGCTCATTGATAAAAACAAACTTTATCCTATCCAAGAAGCATTTGAATTAATTAAAAAACAGCAATTACTAAGTTTGTCAGTTCAGTTGATATTGCTGTTAGTTTAAACCTTGATACTACTAAAGCTGAACAACAGTTAAGAGGTGCAATTGCTTTTCCTTTTAGTATTGGTAAATCTATCAGAATTTTAGCTATCACTGATGATGAGAAAAAAGCTAGTGAAGCAGGTGCCGATTTTGTTGGTGGACTTGATAAGATAGAAGCAATCAAAAATGGCTGATTAGATTTTGATCTAATTATCACTTCTCCCAAATTCATGGGAGCATTAGGTAAACTAGGAAAACTATTAGGAACCAGGGGATTGATGCCAAACCCAAAAACTGAAACAGTTACTGATGATGTAGTTAGTGCTATTAAAGCTTATAAAAAGGGTAAGAAAGAATATCGAACTGATTCATTTGGTAACATCCACCTCTCTTTAGGTAAAACAGATACCAAAACTGAGCACTTGGTTGCTAATGCCATGGCTTTAATAGATTTAATTAAATCTAAACGTCCTAGCACAGTCAAAGGTACTTACATTAAAAATATTGCTTTGACAACAACAATGGGACCAAGTTTAAAAGTAAAGCTACCTGATTAATGCCCACCTATAAACTAATTGTTGGTTTAGGTAACTTAGGTAAAAAGTATGAGAAAACCCGCCATAATGCTGGTTTTATGGTATTAGATAGACTAGCTAGTTTATTCCACTTAAACTTTGATAAAACCAACAAGTTAGGTGATTATCTTTTTATTAAAGAAAAAGCAGCAATCTTAGCAAAACCTGCTACCTTTATGAATAATAGCGGTCTTTTTGTGAAATGGTTACAAGATCACTTTCAAATTCCGCTTGCAAACATAATGATAGTCCATGATGAAATAGCGTTTGATTTGGGAGTAATTAGGCTTAAAATGCAAGGGAGTGCTAACAATCATAATGGCATAAAATCAGTAATTAGACATTTAGATACTGAACAGTTCAATCGTTTACGCTTTGGGATTAAATCACAAAATACGAGTAACATATTGCATGAACAGGTAATGAGTGAATTCCAGAATAGTGAACTGACTAAACTGGAAGTTGCGATTACAAAGTCTGTTGAACTGTTGAAGCGTTATATTGAAGGAGAAGAGTTACAAAGGTTAATGGAATATTATCATCATGGCTAGTGAAAAACAATATATAGCAGGGGTTTCAGGTGGATCTGATTCAATGCTAATGCTTAAACTTTACCAAAAGAAGATTGCTTGTGTTGTTCATGTCAATTACAACACCAGATCAACCTCATTACGTGATCAAAAGTTAGTAGAACAATATTGTCAAAAATTAAATATTCCACTGGTTGTTCACACTGTTGATCCTGATCTAGTTTGAAAGAAAAACTTTCAAAACCAAGCACGGAAAATCCGCTTTGATCAGTTTAAAAAGGCTGCAAAGCTATACCAGACCAACAAGTTATTATTAGCCCATCACCGTGATGATTTCATTGAGCAGGCCAAAATGCAGCTGGATGCAAAAAAACGTGCTGTTTACTATGGGATTAAAACCAGGTGTGAATTGTATGGTTTGAAAATCTACCGTCCCCTAATGAAATACTGAAAAGATGAAATCATTGCCCTCTGTAGACAAGACCATATCCCTTATGAGATTGATGAAACTAACAAGTTACCTATTTATAAGCGCAATGAAGTGAGGTTAGAGATAGAAAAATGGTCTAAAATCGAAAAAGAACAGTTTTATATTGCTATCTGTGCAATGAACAAAACAATTGCTCAAAAACTGTTTGTATTAATGAAAAAAGCTAAAAAATGGTTATTACAACCTGATGTTAGAGAATTGAAACGGTTTTCAATTATTGATCAAAAACAGTTAATTTATAGCTATCTTATCTATCACAAGATTAATGTTAATGGTGAGAAAATTGATGCTATCCTTGATTTTATCCAACCTAGTCAACAAAAACAATACCGCTTGCAAAACGATATCTTTTTAATGGTGAAAAACCAGTGTTTAGCACTTTTATACAAAAGCTAAATGAAACATTTAACTGTCAAAGCATTAGTTTTGCAATTTAATGATTGTATCCAACTCATTGATGGTAAAAACAACATAGATAATGTGATTACTATCCCTGGATTAAAAAGGAGTGTTTTTGAACTGTTAGGGCTATTTTGTAAGCCAATTGGTTCAGTTGCTATTTTAGGCAAACGTGAATTTATTTTTTTAAATCAAAAGCCAGTTGAGCAACAGAAAAAATTATTGCAAACCTCTTGAAGCTCAAACCACCTGCAGTTATTCTAACCAAGTCATTTGTTGATTGTGGTGTTTTGTTAGCTGTTAATCAAACTTATCAAGTTCCTATTTTAAAAACTAACTTGTTTTCAACTGAACTCTCTTTTACTGTTGAAACCTATATTAACGAGCAATTTGCTACTGTTCAAAAGTTACATGGAGTTTTACTTGAAATCTTTGGTGTTGGGGTATTTTTAGAGGGAAAGAGTGGGATTGGTAAATCTGAAAGTGCTTTAGATTTAATTAATAAAAACCACCTTCTAATAGGGGATGATGCTGTTGAGATCTACAGATTAGGCAATAGGTTATTTGGTAGAGCACAAGCACTGGCAAAAGGCTTTATGGAGATTAGGGGTCTAGGCATCATTAATATTGAACGTGCTTATGGGTTACAGATTACAAAAGAACAAACTGAAATCCAACTAGCAATTAGTTTGTTGAGTTTAGAGGAGAAAAACAACGCTAGTTTTGAGCGCTTAGGCAGTGATTTAAAACTAAAAAATCTGCTTGGGGTTAAGATTAGTTACTATCAGATCCCTATCTCTTCAGGTAGAAAAACAAGCAAAATTATTGAAAGTGCAGTAATCGATTTTAAACTTAAGAAAAGTGGTTACAATTCAGCAAACGAGTTTATCTTAAAGCAACGTGCCATGTTAGAAGAACAAACTGATGAATAGACCAAGTTGATCAACTGCATTTAATATTGGTGGTGGATTTCCCATCCAGTGGTATGGGATCATTGTCTCAATTGGCATTATTTTTGCCATTTTAATGTTTGTCTTTAAACTGATTTATTGTTACAAATTACAAGACAACAGTTTTTATTTTTTTATCTTTATTGCTGTTTTAACGATGGTTTTAGGCGCTCGCCTCTGGTCATTTGTAATTGGTGATTCCAATTTTGCTAACAACAATTTCTTTGATTTTCGTAACGGTGGATTGGCCATTCAGGGTGGGATTTTGTTAACCAGTATTGTCGGAGTAATCTATTTCAACTTCTTTTTAAATAGTAAGACCAATAAAACCAAAACGATTGCTAAATTACTGAATAATAAGAATGAAATAAAAGCTGTTTATGTTGAAAGAAATATCTCTGTTCTAGTGATGTTAGATCTGATTGCTCCTTGTGTTTTAATTGGTCAAGCAATTGGCAGATGGGGTAATTTTTTCAACCAAGAAGTTTATGGGTTTGCTTTAGCTGGAACAATGAATGATCCCCAAGCATTGGCTAATACCCAGTGGGGATTTTTAAAGATCTTAATGCCTAAGGTTTGGGATGGGATGTGGATTGATGGTCAGTTTCGCATTCCGCTCTTTTTAATTGAGTCATTTTTTAACACTATTTTCTTTGTGTTAATTTACTTTGTAATGGATTTTATTAGGGGAGTTAAAAGTGGCACAATTGGTTTTAGTTATTTTCTTGCTACTGGAATCATTCGTTTAATCTTGGAAAACTTTAGAGACCAAACCTTTTATTTTCCAACTTCAATAACCACTAGTATTTTGTTTATTGTCGTTGGTATTTTAGGAATTTTTTATTGCCAGTTTATCCATGTCAAATTAAGAAATTACTTCTGAACTTATTTCTTTCTTCATGCCTTTTATAAAGTAGCTGCTTTTTTCACTACACTTTTTTTGAATAACAGAAAGCAAATGGCACAACAGAAGTTTGCTTTTTATGAAAAATCACTTCCCAATAAGAAGCGTTCTTTTTTTGAAATGAAGTATTACAATGATGTAACAACACCCAAAATTTATCGTTTAACTGATCAGGAAATGAAGTTATTTGATAAATTAGAGCCAGTTACAACCAGCTAGATTTTGTTAGAATACTTCAGTTGTCTATATGGCTACAATAGCGCAATTAATTAGAAAACCACGCCAAAAAAAGAAGGTTAAATCAAAGTCACCTGCACTCCATTATAACCTCAACCTTTTAAACAAAAAACTACCAATGTTTACTCACCACTAAAGCGTGGTGTTTGCACCAGGGTTGGCACCATGACCCCCAGAAAACCTAATTCTGCACTAAGAAAGTATGCTAAGGTCAGACTTACAAATGGTTTTGAAGTACTTGCTTATATCCCAGGAGAAGGTCATAACCTACAAGAACACAGTGTTACTTTATTAAGGGGGGGTAGAGTAAAAGATCTCCCTGGAGTTAGATACCATATTGTTCGTGGTACTTTAGATACAGTTGGTGTTGACAAAAGAAGACAACAACGTTCTGCATATGGCGCTAAAAAACCAAAACCAAAATCTTAACTTGATCAGTTAAATAATGAGAAAAAATCGTGCTTTAAAAAGAACTGTTTTACCCGATCCTGTTTTTAACAACACACTGGTTACAAGGATTATTAATGTCATCATGAAAGATGGCAAGAAGGGTTTAGCACAACGCATCTTGTATGGTGCTTTTGAGATCATTGAAAAACGCACCAACCAACAACCTTTAACTGTCTTTGAAAAAGCAGTTGATAATGTTATGCCCCGCTTAGAGTTAAAAGTGAGAAGAATTGCTGGTTCTAACTACCAAGTACCAACTGAAGTTCCCCCTGACAGAAGGATTGCTTTAGCACTAAGATGGATTGTGATCTTTGCTAACAAAAGAAATGAAAAAACAATGCTTGAACGTGTTGCTAATGAAATTATTGATGCTTTTAATAACACGGGTGCTAGTGTTAAAAAGAAGGATGATACTCACAAGATGGCAGAAGCTAACAAAGCCTTTGCCCACATGCGTTGGTAGTTATTTATTATGTCAAGAACAGTTGATTTAAAAAACTTCCGTAACTTTGGCATTATGGCCCATATTGATGCTGGGAAAACCACCACATCAGAACGTATTTTGTTCCATTCAGGTAGAATTCACAAGATTGGTGAAACCCATGATGGTGAATCAGTGATGGACTGGATGGAACAAGAAAAAGAAAGGGGTATTACTATCACCTCTGCAGCCACTTCAGTGAGCTGAAAAAACTGCAGCTTAAACTTGATTGACACTCCTGGCCATGTTGACTTTACAGTTGAAGTGGAGCGTAGCTTAAGGGTTTTGGATGGAGCAATTGCGGTATTGGATGCTCAAATGGGAGTAGAACCACAAACTGAAACAGTATGAAGACAAGCTTCACGCTATGAAGTACCACGGGTAATCTTTGTTAATAAGATGGATAAAACCGGTGCTAACTTTGAGCGCTCTGTTTTATCAATTCAACAACGCTTGGGAGTGAAAGCTGTTCCTATTCAATTTCCCATAGGTGCTGAAAATGATTTCAATGGCATCATTGATATCATCACTAAAAAAGCTTATTTTTTTGATGGTAATAAAGAGGAAAATGCTATTGAAAAACCAATTCCTGAACAGTATGTTGATCAAGTTGAAAAACTTTACAACAACTTAGTTGAAGAAGTTGCTAGTTTAGATGATCAACTCATGGCTGATTATCTAGATGGTAAACCAATTGAAATTGATGCAATTAAAAATGCAATTAGAAACGGGGTAATTCACTGTAAGTTTTTCCCGGTATTGTGTGGTTCAGCATTTAAAAACAAGGGAATTAAACTCTTACTTGATGCAGTGGTTGATTTTCTCCCTTCACCTGTTGATGTCCCACCTGCTAAAGCAATTGATGCAAACAACAAAGAGATATCTATTAAAGCTAGTGATGATGCTAACTTTATTGGCTTAGCATTTAAAGTTGCTACTGATCCTTTTGTTGGTAGATTAACTTTTATTAGGGTTTATGCAGGGGTTTTAAAATCTGGTTCTTATGTTAAGAATGTTAGAAAAACAAAAAGGAAAGGGTATCACGTTTAGTGAAAATGCACGCACAAAATCGCAATGAAATTGATGAAATTAGAGCAGGGGATATCTGTGCAGTAATTGGCTTGAAAGATACTACTACTGGAGAAACTTTAACTGATGATAAGCTTGATGTGCAACTAGAAGCAATGCAATTTGCTGAACCAGTGATCTCTTTAGCAGTAGAACCTAAAACTAAAGCAGATCAGGAAAAGATGTCAATTGCTTTATCAAAACTAGCAGAAGAAGATCCTACTTTTAAAACCTTTAGTGATCCTGAAACAGGGCAAACTATTATTGCTGGAATGGGTGAGTTACACCTTGATATCTTAGTTGATAGGATGAAACGTGAATTTAAGGTAGAAGTTAACATTGGTGCACCTCAAGTTAGCTTTCGTGAAACCTTTAAATCAACTAGTGAAGTTGAGGGTAAATACATCAAACAATCAGGTGGTAGAGGTCAATATGGACATGTTAAAATCCGTTTTGAACCTAATAAAGATAAGGGCTTTCAATTTGTTGATAAGATTGTGGGCGGAAGGATTCCAAGGGAATATATTAAACCAGTTCAAGCTGGTCTTGAAAATGCAATGAATTCAGGTCCTTTAGCAGGTTACCCAATGATTGATATTAAAGCTACCTTATTTGATGGTTCTTTCCATGAAGTTGACTCAAGTGAAATGGCTTTTAAAATTGCTGCATCCTTAGCTTTAAAAGAAGCAGGTAAACAATGTAACCCAGTTTTACTTGAACCTATTATGGCAATAGAAGTTACTGTACCTGAACAGTACTTTGGGGATACAATGGGTGATATCAGTTCAAGAAGAGGGATCATTGAAGGTACTGAACAACGTGATAATGTTCAACTAATAAAAGCAAAAGTACCTTTAAAAGAGATGTTTGGTTATGCCACTGATTTACGCTCTTTTTCCCAAGGTAGGGGTAATTATGTAATGCAATTTAGCCATTATGCTGAAACTCCTAAAAGCGTTGTTAATGAGATAATTGCTAATAAAAAATAGATACTTAATAAAAATATAACTTTACTCAATGAGTTTTTCCAAAAAGTTTTTATGCACTACAATATCATTCTTTTAGTTGATGGTACGCTTAGTTTAGAACAAGCTAACCAAGTTGAACAAAAACACCAAAAATTGCTTGAAAAGGCAACTGAATTTAAAAGTGAATACTTAGGTTTAAAAGAGTTGGCTTACCCCATTAAAAAGCAACTTTCTGCTCACTATTACAGATGGAGTTTTCATGGTGAAAGCAATTGTACTAAGGAGTTTAAAAGAGCTGCTAACATCAATAAGCAGATAATAAGAGAGTTAATTATTAACAGAGAAAAAGACTATGGTTATTTAGGTTCAGTTAACCCTAAAAAACAACAACTGTCTTTGCAGAAGCTAACCAAGTATAATGAGATTATTGCTAGTGAAAATAATCCTGATAACCCAGATGCGCCTGTCACTTCTGGTCTAGCTTCTGTTAAACCACGGCTATCAAGAGTTGAAAAACAAAAGGAACGTGAACTTGAAAAGTGAACGGTTGTTCACCAATCAGGTAACTTTGATACTGTACAGATCAATCCTTATCGTCCTAGGATAAAACGCTTTTTACAAAACAACCAACAAACCTCCCAAGCTAATAATAACCAACCTCGTTTTCAAAATCAATTTAAAAAAGGAGCAAAACCTTAGATGAACCGGGTCTTCTTGTTTGGTAAACTCAGTTTTACTCCCAACCGTTTACAGACAAAAAATGGTACGTTAGGAGCTACTTTTTCCATGGAATGTCTTGATTCCAGTGGTTTTAATAATGCCAAATCATTCATTAGAGTAACTGCTTGAGGTAAAGTTGCTAGTTTTATTGTTGCTCAAAATCCTGGGGTGATGCTTTTTGTAGAAGGAAGATTAACTACATATAAAATTACTAACAGTGAAAATAAAAACACCTATGCTTTACAAGTAACTGCTGATAAGATCTTTCATCCTGATGAAAAAACTACCAATGAAGAACCTATTAAATCAACTGTAGTTGATTCACCCTTTATGAATCCCAAAGCAAGTGTTACAGAAGCTGAGTTTGAACAAGCATTCCCCCATCAAGATGAAACTGATTTTAACAACATTACCCCTATATTTGAAAATGATGTCCAACTAGAGGAGGAAAGTGATGATTAATAAAGAACAGGATTTAAACCAATTAGAAACCAACCAAGAACAGAGTGTTGAACAAAACCAAACTGATGAAAAGCGCAAGCCAAAACCAAACTTTAAAAGAGCAAAAAAATATTGTCGATTTTGCGCCATAGGTCAACTAAGGATTGATTTTATTGATGATTTGGAAGCAATCAAACGCTTTCTCAGTCCCTATGCAAAGATTAATCCTAGAAGAATTACAGGTAATTGCAACATGCACCAACGTCATGTAGCTAATGCTCTAAAACGAGCACGTTACCTAGCTTTAGTGCCATTTATTAAAGATTAAATATGAAGATAATTTTGAAGCAAGATGTTGCTAAATTAGGCAAGCGGTTTGATGTTGTTGAAGTTAAAGATGGGTTTGCTATCCATTTTTTATTTCCCAAAAAACTAGCTGCACCTTTAACAAAGAAAGCAATTGCTAACCGTGATTTGTTTTTAAAACAACAACAAGAACAATACCAAAAAAATCGTGCCTTAGCTGAAAAATTGAAACTAGTAATTGAACAAACACCATTAACTTTTCAACTCAAACAACACGATGGCAAGCCATATGGTTCAATCATCACCAAACAAATAATTAATTTAGCAAAACAACAAAGACTTGATTTACAGCGCTTTATGTTTAAAGATAATGTGCGCTTACAGTTTGGTGAACACAAACTAATTTTGCACCTTTTTGAGGAGATAACTGCAACTTTAACTGTTATAGTGAACCCTGAAAATGGGACAACAAACTAGTTTTAAATATGCAAATGATAGCAACATTGAACGTGCTGAAAGACGTTTGATGCAAGCAGTTGCTCAAAACAGTGAGGGCATTGATCTAATTTTCAATAAACTTGAACCAATTGATTTTTTTGCAACCCCTTTCAAACTCATTTTTCAAACTGCAAAAGAAAACTACCAATTAAATAACCCTATTATTGGTTCTGGTTTACTAGAAGCGGTTAAGTTTAAACTTGATGCTAATGATCAATCCACTAAAAGTGAACTTGAAATTTTATTCACAAAGATCTTATTAATCCGTTTACCACCTAACCAAACAGAGATTAAAACACTGGTTGATGTTGTTAAAAAAGCTTCTATTTTTCGCAGGTTACAACAGTTTGCTAAGCGTGTTTACAACGAGGAATTTAAGTTAAAAGAAGATCGTTTTGAAGGCTATTTACAAGCTATTCAAGATGATTTTGTCAAGATTATCCACAGTGCTTTTAGTAACATCTTTGCTTTTAGCTATGATGAGATTGCCAATCAAGAGGAAGCATTAATTAAAAAGGTTCACCGTGGGGAATTGATCATCAGTGGACTTTCAAGTGGATTTTTAAAATTAGATCAACTTACATCAGGTTGAAAACCAGGAGAGTTAATAGTAATAGCAGCTCGCCCAGGTAGAGGTAAAACTGCCCTTTTGATTAATTTTATGGCTAGTGCAGCTAAACAAATTGATCCTAAAACTGATGTGGTCCTCTTCTTTAGTTTAGAGATGCGTAACCGGGAAATTTACCAAAGGCACTTAATGCATGAAAGTCAAACTAGTTACACACTAACCAACCGGCAAAGGATTAATAATGTCTTTGAAGAGTTAATGGAAGCATCTTCAAGGATCAAAAACTTACCTATTAAACTCTTTGATTACAGTAGTTTAACACTCCAAGAGATCAGAAACCAAATTACTGAAGTGAGTAAAACCAGTAATGTTAGGTTAGTAATTATTGACTATTTACAACTTGTTAATGCTTTAAAAAATAACTATGGTTTGACAAGACAACAAGAAGTGACAATGATCTCTCAATCACTTAAAGCATTCGCTAAGGAGTTTAATACCCCTATTATTGCTGCAGCTCAACTTTCTAGAAGGATTGAAGAAAGGAAAGATTCCAGACCAATTCTTTCTGATTTAAGAGAATCAGGTTCAATTGAACAGGATGCGGATATGGTTTTATTTATCCATAGAACTAATGATGATAAAAAAGAACAGGAAGAGGAGAACACAAACTTGTTTGAAGTGGAGCTTATCTTAGAAAAGAACAGAAATGGTCCCAATGGCAAAGTTAAACTAAACTTTCGCAGTGACACTTCTTCTTTTATTAGTCAATATTCCCCTAGTTTTGATGACCAATACAGTTAATTTATGAAACTGAAGTTTTACAAACTACCTTTAATTACTACAGCATTTAGTTTTGTATTTTTAACTGCTTGTTCAACACCCCAATCTTCCTTTTTTCTCCCTGCACAAACAACTATTAGTACTTTAAAAATTAATGGTATGGAAAACAAAACAGGTTATTTTCTTGAAACGCAGCGTTCTCGTGGTAGTTACAATCCCACCGCTTCACTTACTATGATTAAGTTAGGAGATGAAAAAGAGTTTAAAAACGTAGATACAACCAAGCAAGATGAAGTTTTGTTTGCCAACATTTATGGTGGAATCTCTTCATTGCTTAACTTCAGAATTATCCAACCGATGTTAACCTACTGAAACTTAGTTAACAACTCATTAAGTCAGATTGGTAGTACTAATGACTTAATTACATTCAAAGATAGTGGATATAAAGATCAACTTGCAAAAGCGCTTGCTAACAATCTCATAGTTGCTGATGAAGGTAATAATAACTTTTGGTTTGGTTTAAAAGCCCTAAAGTTTGATACGGTTAAACTCCAAGCTAATAATACTGCTACTAGTTCAACAAGAGCTTCAACTACTCAAAATACTAATAATAAGATTGATGCACGTGAAAAAATCACCATTAATGGGAATGGTGGAACAAATAATGATCAAAATGCTACTGTTCAAAAGTTGATAGGTATTGAAAATATTGAAGTTGAGTTTAGCTTTGTTAAAACTGGTTTTAATGGAAATGAGATTAAGTTTGAAGATTATGTAACAGATTCTTCCCCCACAACATCTTTATTAAAGCAAGTTTGAAAAAGTAAGTGAAATACCGAGTTGACCCACACCAGCTTTAAATTCAAACTCAATTCTTTCAATGTTTTGTTAACTTATCAATTGGAAGCAAACCAAAAAAGCCAATATCTTCCCAATGGCTTTAGCTTTCTTTTTCCATCTAATTTAGAAGGAAAGATAGATAGTTCAAAAAGCTATTGAAACAATCTTGTTGATTTTTCAAAAAAAACCACAAATGAAGAGAACACAATGCTACTAACTGATTTGCAAAAGAAACAAGATCAAGTGAATCGGTTTGTTGGTTTTATTGGTCAAAACCACTTTACTTTAAGTGCTAACAGCATTAATGAAAAGCAGTTTAACGATGCTAGTACTGCTGATTTTTTTAAAGCAATCTTTCAAAAAGTGAGTATCAATGAATAACAATTTTAGCCATTCAGTTTTGAAAAGAAAAGTTTAAATTAATGGTAAAATTATTCATTAAACTTTCCTTGTTAGTTGTAGTTTTCAATACTGTTTGTATTTCGAAGTTCATTTTAAAAAATTCGTAGTTCTAGATCTTTAAAATATGTTTATTTAATTGTCTTTTTTAATTTCATAAATTGTTTGAAAACACAAAAAAATATCTTTAAGGTCAAGGCCTTACTACTTTCACTTTTTTCTGCTGCTGTTACCATCACTATTTTTGCTTTACCTATCTTTGCAAACAATGGTTCTAAAACTGATTTAGGTTTGCTTTCTAAAAATAGTGCTGATTTTTTAGGTAGTTCAAAAAGAAGTCTTGCTGGCTTTGATACTCCTTTTAGTCCAGATAACCTCCAGTATTTAGAAAAAGAAACTGACTATGATCAGAACTTTAAAAATTTTACTGAAAAGTTTAAAGATGAAAAAATAACTAACAATCAACTTGATATTGTTGATATCTATAACCTATTCAGTGGTTTTCACAAGAGTGTCAAAAACACAGTTGACTTAATGAGCCAACTGCAAAAGCAAGTTGAAGCTGCCAATGCTATCTTCCCAGTTGATGATGCTTTTGTTAAATTACCTAAAGTACCTACTGAATTATTTAAGCTAGTTGATGATAATGTCTTTCCTAAGTTAAATCCTAAGGGCTTAAATATCTCTGATAATATTGCTGCACTTTTTGAAAGATATAACCTTAAATCGATTGAACTTAAAAACTTTGATTTAGCTTTCTTGAGAAAAGCTGATGTAATTATCAAAGATAAGGTTCGATACAACTTTGAGATGCAAATGCAGTTTCAAACTGTTTATGTTGGTGGAGGGAATACCGTTATTAACTTAGACTTTACTTTAAAAGCCCAAACCGTTAACTTTGCTAACCTCCAAGATTTACAAAACACTTTTGTTAAAGTTGGTAATGATCTTTCCACCCAACTCTTTTGGATTCCAACTGTTAATAAATTAACTGATAATGCAGGTAATGATCTTACCCATATTGCTAAAACTGTGATTGGTGAATCGTTTTTCCAAACCAATGTTAACTTAGCTAAATCAGTTATTGAATATGATAAGGTTCAACCATTGGTTAAACAAGCTTTTGAAGAGCGAGTTTTAACTCCTTTCAAAAAGGAAAGAGAAGCTGCTAAAAAAGCTTATGAAGAAGAACAACGTCGCTTGGAAGAGGAACGTAAGCGTCAACTAGAAGAGCTAAGGAGAAGAGAAGCTGAGGAGAAAAGAAAAGCTGAAGAAGCAAAACGAAATCAAGAAAAAGCACGCAGAGAAAGAGAAGCTTATGAAAAATCATTTAACTCCTTTAACTCCTTTAAATTTTACTGGTTAACTAAAGGTAAAGATGTTACTAAAAAAGCTGATTTAATTGATGCACTTAAAACCGCTATTGCTACACCAGCATACAGAAATAGAACATTCTCTTTATTAATCAAAGGTTTTGCTAGTGGAGTTGAACGTTATTTCAACGCTAACAAGAATGATAAAGAGTTGAAAAAACTTGCATTTGGTGAAAAAGGGATCCAATTCCCTAGAGCAGATGCTGGGATAGATGGTCTTTACATGAGTAATTTTTTGAGGCATGAATTGACAAGCAAAGCAAAATTTTCACTTAACCTAAAAGATATAAAAGTTGAAAATACGGTTGAAGATACTCAATTGTATTGGAAGGATAATGGCATTCATTTAAAGCAAGCCAATCCTTATAAATTCAATCTAAATATCAAGATCAAGTACAACGGCTGGTATAATGTCCACTGGTGAAACTGGCTCCCTGCTAAAATTTTAGGGATTCCTACAGACTGAAGTGGTGAGATGAATTTAACATTTGTTGTCAACGGTGACCTATCAGAAATTGTTGATAAACATGATTACCCTGGTACTTTCTTTCAATTTACCGATAAAAATGAATTGCTATTTACCTTAGCAGTTAGAGAACAAATTAAGGTTGATAATAATCATTTTATGGGTCTGTTAAAAAGCCAAAACCTTCATAATCTTCAGCTTGCTTCTGGTGCTACAAAACCTCCTGTTGTTGATTTAGCTAGTTATTTCCACTTTGTATTATTGACTGAAAAATCTTAATCTATTAAGAAATTTTTGATCAGTTACTTTTATCTAGATAAATTTAAAGTTTATTTGTGGTAATGGATGATCTATTCCAAAGAATGGTTAGCTGTGTTCTACCGTCATGAAGAGCTTTTATTGATGAGGAAGTTAAAAAACCTTATTTTCAAGCTTTATTAGAAAAATTAAAGGCTTTAAAAGCAACAATAATTCCAAAACCAGAACTTATTTTCCGTGTTTTTAGCTTCTTTAAGCCAATTGATACAAAGGTAATTATCTTTGGTCAAGATCCCTATCCTAATCCTAATGATGCATGTGGACTTGCTTTTGCATCCAATAATTCCAAAACCCCTGCCAGCTTAAAAAGAATAATTTTACGTTTAGAAAAAGAATATCCTTCGCTTAAACAAGAAAGTAGTTGACAACAAAACTTCCTATTGAATTGAGCAGAACAGGGCGTTTTATTACTAAATGGAATTTTAACAACTACTGTATTTATACGCAACGCCCATAAAAATTGGGGTTGGGAGGAGTTTAACTGTAATTTGCTAACTTTTCTAAAAAATCAAAACATTAAACCGCTGTTGGTATTTCTGGGTGTTCAAACTAAAAACTTTGTTGTTAAGAGTATTGGTAATGTTGATGGATTTGAGCATTTATCATATCCCCATCCCTCACCACTAAGTGGTAATTTGTTTCTAACAAACCCTAACGATCTGTTTAAAACAATTAACAATTGGTTGAAACAACATAACCAAAAAATAATTAACTGAGCAGTTGTTAAAAATGCTAGTTTTGACCAATTAAGTTAATTAAAAACAAAGCTGTAGTTGACACTTGTTTATTGATTAGAAAAACAACAACCTTGTAAAATTATTTAACTAAGTGATTATTACTTAATTGCACTTTAGATGGGAGAGATTAAAAATACTGCACCAACAAGCGATATTTCCACTTCAGGATTTATCTATTTTGCAGTTGTTTTTCTAATCATTATAGTCTATCTTTTTTTTAAAAACATTCTCTTTTTGTTCTTCTTTAAAAGATATCCTAAAAACACACCTAAAATTGGGGTTAGCAATATTACTACTATTGCTATGATTATTGCTGTTGCTGTATCTGTTGTTTTGGTCTTAATGGCTTTAGCAGGGGGGTTAACAGCAGCGCTGTTCCGTGGTTATCCTGGGTTCCGTGTTACCTTAGAGTTAATTCTAGTGAAGATTTCAGGACTTTTATTTGGTCCTATAATTGGTATTTTTTCAGCAGCTACCATTGACTTTTTAACTGTTATTTTTTCAGGTGGGGTGTTTAATATTGGTTATGTTTTAGGAGCAATCCTAACGGGAATGATTGCTGGAATTTTACGTGAAGTTTTAATTTCAACTTCTTTTTTAAATAACAAAACTTTAAGTGATTTTGCCTATCTTGTTTTATCAGTGGGGATGGTATTTGCCAGTTTTTTAGTAACCCAGTTTTTTGTTATCTCAGTTACCCAAAACTTATCAGCATTTCAAAGTAATGATCAAATTGTTTTACGTTTTAACGCCTCACCTTTAAATTTCAGTATCTCATTGCAAAGATATGTTCAAATTATTTTCTATTTTGCGATGGTTGTAATCATCACAATGGTAGTACTTTACTTTGTTTGAATAATCAAGCAAAAGCACTTTAACTATGCTTATTCCAAGTTTTTCTTTCGTAGATATAAACATGCTAATCACCAATTTACCTTATTTGTGTTAACAAAAGAAAACTGGTTTTATCTAATTCTGAATGTAATTACATTAGCAACCACCAGTTTGCTAATGATTAACATTGCATTTATTCCTATCTTTGATACCCAAACAACTGGACAAACTTATGATTTTTGATTATTAGTTAGATTATTGTTTGCTCCTTTGATCTTCTTACTTGATATTATTGTTATCTATCCAATCTTGTTATTGTTAACCCCAATCATGTTAAAAGGGTTTAAAACAGTAGCATCAGAAACCCAAACAAAAGGAATCAAAAAGAGTTTTTCAGATATGCAAAGCTTAATTATGCCCAATGTTATTAGTCACAAAAAACAGCAGTTAATTAGAAAAGAGATGCAACAGTTAGCAAAAACAATCAGAATTGATTTATCAGACAAAGAAGTGGATGCACTTGTTGAAGAGTTTAAAGAGATCACAAAGAGTTTTAATAAGGTAACTAAAATTGATACCACTAATGTTCAACCGATGTACGCTCCATTTGAATTTAGTCCAACCCCACTAAGAAAAGATAAACCAGTAGTTGATAAACACGCTAAGCAACTACTTAATAACTGTTGTGAAGTTAAAACAGGTTTTGTAAAGGTATAGTTGTGCGATCAAATATTTTAAGTCTCAGGGCGATACTTGATAAAAAACCTAGTGCCATTAACGATGTTTTAACATCAATTAATGCAAAGATAGAACTGAATAAATCAAGTAATTTTTTATTGAAAAATACTGTTGAAATTTATTCAAAAAAAATTAATAAAAGTGATGAAAAGATTCTGCTAAATAACATCCCTTATGTTTTGAAAGATAACATCGCTACTAAAGATATTGTCACCACTGGTGGTTCTTTGTTTTTAAAAAACTATCTTCCCCCTTTTTCAGCAACTGTGTTTGAACTGTTAGAAATGAATGGCGCGTTGCTTGTTGGTAAAGCTAATATGGATGAATTTGGCTTAGGTGGAACAGGTAGTTATTCTGCTTTTGGTGTTGTCCATCACCCTGAAAATTCCAGTTTAATTGCAGGTGGTTCTTCCTCAGGTTCAGCTTACGCAGTTGCTAAAGACATTGTTCCTTTTTCCATTGCAACTGACACTGGTGATTCGATTAGAAGACCTGCTAGTATCTGTAATGTTGTTGGCTTTAAACCAACTTATGGTTTGATATCACGTAATGGGGTATTTCCATATGCCCCAAGTATGGACCATGTGGGGATATTTGCTAAGTTTGTTAGTGATATTGCCATTGTTAGTGATGTTGTTATTAAACATGATAAAACTGATTTTTCTTCCCAAAAATCACCTGATGAAAACCAGTTTTTCAATGAGTTGGCCATTCCCTTTACAAGATCAATTCGCTTTGGTTATTTAAAACCACTAGAAAAACTGTTTAACAAACACCTCCAAAAAAAATGAAATAATCTCAAAAAAACCTTAGAACAAAAAAACTACCAGTTGATTCCACTTGATTTTGATGTGGAACTTCTCAAAGTAATTGATTCTATTTACAAAATAATTAGTTATAGCGAAGCAGTTAGTTGTTATAGTAATTTAACTGGCATTGTCTTTGGTCAAAAGGTGTTTGAACCTAATTCACCAAGTAATTTTGATCAAACTATTACCAGAAACAGAGATCAGTTTTTAGGTAAACAACTAAAAAGAAGATTTGTAATAGGGGCATTTGCAACTGATGAGAAGAATTTTGAAAAGTACTTTGAAAAAGCTCAGAAAATAAAAAGAGTCTTAGTGGATAACTTTCTGAATCTCTTTAGTGATGTTGATTTTGTATTATCACCAAGCGCTTCTTGTTTTGCTAGTACCATTGAAGATATTCAAGCTAATAAGCCATATACAAACATTATTGATGACTTTTTACAATTAGCTAATTTTGCTGGTAGCCCTTCTATAACTATCCCTTGGTTAGTTCAAACAAAAGACCAAACAATTGGTTTAAGTATTAGTGCTAACTGTTTTGAAGATAAAAAACTCTTACAAATTGCTTATTGATTTGAACAACTTTTTGATTTAAACCATGATTAATTTTGAAGCGATTATTGGAATTGAAGTCCATGTAGTTTTAAATACTGCTAGCAAGATGTTTTCAAAAGCACCTAACCGCGTTGATAATCAAAAAATCAATCATTTTATTGACCCAATAGATTTAGGTTTACCAGGCACTTTGCCTCAAGTTAATGAGTTAGCAGTTTACAAGGCATTATTATTAGCTGATGCATTAAAAATGAAGACAGTAACAAATAAACTTGTTTTTGATCGAAAGCACTATTTTTATCAGGACTTACCTAAGGGTTTTCAAATCACCCAACAAAACTATCCTTTTGCTAAAAATGGTGTAGTTACCATTAATGTTGATGCTATTGAAAAACCAATTTATATTGACCGGTTTCATTTGGAGGAAGATACTGCCAAACAACACTTTAACCATGACCAAATTCTGCTTGATTTTAATAGGTGTGGTGCACCTTTAATTGAAGTTGTTACTCTTCCTGTTATCAACACTGCAAAAGAAGCGAAAGCCTACCTACAGAAGTTGAGACAAATTCTGATTGTTAACAATATCTCCAATGCCAAATTGGAAGATGGTTCAATGCGGAGCGATTGCAATGTTTCAGTACGTTTAAAAGGTCAAAGGCAACTAGGAACTAAAATTGAAATTAAAAACATCAACTCACTTAATAATGTTGAAAAAGCGATTGATCTTGAGATTAACCGCCAAGTTAAAGCACTTATTAATGGTGAAACCTTGAGTCAAGCAACTTTAAGCTTTGATGATAAAACCAACAACAATGTTTTTATGAGAAAAAAAGACAATACGATTGACTATAGGTACTTTATTGAACCTAACATCATGACTAGTAATATTGATGATTTATTATTAGAAAAACCTGTTGCTTTTCAGTTAGAACAGTTTCAAAAAGAACTAATTGATAGTAATGTCAATCCTCAATTAGTCCAATTAGTAGTTGATGATGCAACTATCTTTAGTGCTTTTCAAACTATTAATAGGGTGGTTCAAAACCCCCAAGAAACCATCAGGTGGTTATGTATTGAACTAATGGGTCAACTCAATAAAACCAACAGTTCATTAACAGTTAATTTAATTCAAGATCTAATTACCCTAATTGAAATGCTAAAAGCAGCAAAGGTTAACCAAAAACAAGCTAAGCAGTTAATTACTTTAATGATTGAAACTAAAAAAGATCCAAAATCGCTAGCTAAGCTCCATAATTTAGAGCAAATTACTGATCCAAAAGAGTTACAAAAGATCATTAAAAAAATCTTTCAGGAAAATGAAAGAGAGATCCTGAAAAACATTGATAGAATTGAACGGATTCAAAAGTTAATTATTGGTCAAGTTATGCATAAGACCAATAATCGAGCAAACCCCCAACAAGTTTTTATAATTGTTGAAAATATGCTTCATGAAGTTCGGGAAAGAGATAGCTAAAAAAAATCAAATTATTTATCGCTATATAATCCTTAAAATTCAAAGCTTTGAATGACCTGCTAACACCCGTATCTTCTCAGAACGCCAACTAGAAATCCGTTTCAACTCCTCACGGAGTCAAATTCGCTCTGTTCTCGCTACGCTTTTAAACAAAAATATAATCCGCTACACTAAAAATACCCCTGGTTATTTTGTGTGTAAAGATGTAGGCTTTAGTTTTTTTCATAAAACCCAGGATAATCTGAAGGTGAAATATGCTAAACTTTCAACTTTGATTAAAAAGCTGTTATCACAGGATGATGCTAGTGTTTTTGCCAATATTGACAGTACTGTGCACCTTGATAAATTTAAGGGAATTGAAGCTAAGTTTTTTGATGAGAATAAAAAACACTTTTTAAACGTTTGTTTTTTTGCTAAAGATGATATTTTAAACATCCTTGATGAAAACAACCTCCAGCAACAGTTCTTCAGGGAGTTTGCTTACAATGGGATTGCTATTGAAAAACGTCATTGTGTTACCTCTGTTGACAAGGAAAGTGGGTGCTTGGTGATGTATGACATGTATTATGATGACAATGATAATTTTGTAGTTGCTAGCAAAAGTAACTTCCTTAACCCTGAATTGAAAGTAATCAATGCTTAAAGTGAATGCTGATTTTTTAACTAAAGATCAAGTTATCTATGATTTAGTGATAGTAGGTGCTGGCCCTGCTGGGATTGCTAGTGCCATTTATGGTAAACGTGCTAACTTAAATTTAGCAATTATTGAAGGAAACACTCCAGGAGGGAAGATAGTAAAAACTAACATTGTGGAAAACTATCCTGGTTTTAAAACCATAACTGGTCCTGAATTAGGTCTTGAGATGTACAACCACTTGTTAGCATTTGAACCAGTTGTTTTTTATAACAACTTAATCAAAATTGATCATCTTAACGATACATTCATCTTGTATTTAGATAACAAAACGACAGTTTTTAGCAAAACTGTTATCTATGCAACAGGGATGGAAGAGAGAAAACTTGGCATTGAAAAGGAAGATTATTTTTATGGTAAAGGGATTAGTTATTGTGCTATTTGTGATGCGGCTCTTTACAAAGGTAAAACAGTTGGTGTTGTAGGAGGAGGTAATTCTGCAATACAGGAAGCAATTTATCTTTCAAGTATTGCTAAAACAGTTCACCTTATTCACAGACGTGAAGTGTTTAGAAGTGATGCATTACTAGTTGAAAAATTAAAAAAAATTAGTAATGTAGTTTTTCATTTAAATGCTACTGTAAAACAGTTAATAGGTCAAGAAAAGCTCCAAACTGTTAAATTGGCAAGCACAGTTGATAAATCAGAAAGTGAAATTGCAATTGATTGTCTCTTTCCTTACATAGGCTTTGAAAGTAATAACAAGCCAGTTTTAGATCTTAAGCTTAATTTAGATCAAAATGGTTTTATTTTAGGAGATGAAAATATGCAAACTAACATTAAGGGTTTTTATGTTGCTGGGGATTGTAGAAGTAAATCATTCCGGCAAATTGCCACTGCAATTAGTGATGGGGTAACAGCTGTTTTAAAGGTTAGGGATGACATTTAGTACACAGATTAAAGCTGAACTGGTCCAAAACAAATTAATTGATAAACACTGAAATGTCTTTTTAGCAGGTTTTTTTCAAAACAACTTAAAGCTACTTTACAACCGTAACTGAAGTTTTAAAGTGCAATCTGAAGCATTAAAGGAACAATTTGTTCAAAATCTTAAGTTTGACTTTAAGACAAAAGCTAGTAAGAAATACTTTCTTTTTGAGTTTAATGCAGATATTAACGTAATTAACACTCTTTTAAAACTTGATGTGACAACTAGTGAATTGGTAGTTAAACAAGTTTATCTCATTGCTGCTTTTTTAAGTGGAGGTAGTGTTAGTGATTTAATAAACTCCAATAACTTTCACTTGCAAATCAGTTCCAACAATGAGTTTCAAATTCAACAACTTTTAAAATTGTTTAGTTTTTTTAAAAAAACAGTTAAACAAAACCAGTTAGTTGTTTATCTTAAAAGTTATGAAAAGATCTGTAATTTTTTAAAACTGATTCAAGCCTTTGATGGTTATCTTGCTTTTGAAAATAAGCAACTAGAGAAAAGTTTTACTTTAAACCAGTTAAGAAAAAGTAATTTGGAAGTTGCTAACTTAATGAAGACAATCAGATCTAATAATCAAACTAATCAACTCCAACTAAAATCATTTATTAAAAGTAGTAGTTTTGCAAAAAGACCGCTTAATTTTCAGCGTTATTGCTTAATTAAAAGTGATCATCCTGATTGATCTTTAGAACAGATAGCAAACTTTTTTTTCACAAAATATAACATAAAGATTAGCCGCAGTGGAATCCAACATTTTAGTGTTAATCTAAAAAAACTATGCCAGTAGTTTAAAATTAGTTCACAAGCAATGCATCCAATCCAAATAGTAATGTTCATTATGGCTGTTATCTGTTTAATTATTGGACTTTTGCTTTCTAACCATGGTTCTACTGGAGGATTAGCTTCTCTATCAGGTCAGGACTTGGAAATCTTTCGTAAAACCAAAGATAGGGGTTTTGTAAAGATCTTACAGATTATCATGTTTATCTTAGTAGTTTTATTTTTAATTCTTGGGTTGATATTTAGTTTTGCACCAAGATAACAATGAAGGTTCTAACTGAACTCCAAAAGCAGATATTTACCATTGTCAAAAAGGAAAATGGTAAACCTATTCCCCCTGGAATAGTGGTAAGAATGATGGAAAATAGTCCTAATTTTCCAGGTAAACATCTCATCTATCGGGCCATTGATGATCTGCTTGATTGAGCCATCTTAAGGAAAGCTGGTGGGGTTACAAACCAGCTATTAGTTAACTATGAACCTGCTGAGCCTTTACTTGATAAAAAACTACAAGGGATTTTAACCTTAGGAAATAAGAATAGTGGTTTTATCCGCTCTTTGGATGATGATAAAACTGTGTATTATGTCCATTACTCTAATTTAACTGGAGCTTTAGATGGGGATCTTGTGGAGTTTTGTAAATTAGATAAACCCCAATTTGGTGATAAGTTTGATGCTGCAGTTATTACTATTCTAAAAAGAGCAAGAATCTTGTATGCAGGTAATTTTTTAGTAGATCAAAATGAGTTTGCCTTGGAATACAAAATTGTTGCTGATAACCCTAGATTTTATTTAACTACGATTGTAAATCCTGATTCTATCCCAAATAACTTAGCATCTAACACCAAGATAGCTTTTCAAATTGATGAGTATGATCCTGATAACAACTTATGTAAGGTTTCTGTACAACAAGTTTTGGGTAACAATGATGATCCGCTAATTAATATAAAAGCAATCATGTTGGACAATTCCATTGTCTTTGAAACTAACGATGTAGTTGAACAGCATGCTAACAAGTTAAGTTTTGATACTGAAGAACAACATAAAGCTTACCGTCAGGATTTAACTGATTTAGCTTTTGTGACTGTTGATCCTACAACATCAAAAGACCTTGATGATGCTATTTATGTCAAAACAATACCAACAGGTTTTGTGCTTTATGTAGCTATTGCTGATGTTGCACACTATGTTAATAGAAATAGTGAAATAGACATTGAAGCAAAACACAAAACAAGCTCAATCTATCTACCTGGTCATTATGTTGTGCCCATGCTACCTGAGCAATTGTCAAATCAGCTCTGTTCTTTAAATCCAGCACAAAAACGTTATGTTGTTGTTTGTGAGATTAGTTTTGATAATCAGGGAAGGATTAAAACAAACAAGCTTTACCCAGCAACAATTATTTCCAAAAATCGTTTTAGCTATGATCAGGTTAACAAGTGGTTAAATAATAAATCAGAATTAAACTGTGATGAAACAGTTATCAACAGCTTAAAAGCAGCTTTTACACTAAGTGATCTAATTCAAGCGCAACGTCAAAAACGCGGTACAATTGATCTTTCACACAAAGAAACTGAGATAGTTGTTGATGAACATTATTTTCCCATTAAGATAAATTTTTTGGTTCACGATAAAGCTGAAACCATGATTGAAAATCTCATGGTAGTGGCCAATGAGACAGTTGCTTGGGTGTTAACTAACAACAAAATTGCTTTACCATACAGAGTTCACCCAAGACCAAGCAAAAAGAAGTTACAAAGTTTGATTGAAACAGTTGGTGAGTTGAACATAACTAAACCCCAATTTAACTTAGATACTGTCACTTCAAGCCAAATAGCTAGCTGATTAAATGAAAACAAAGATAATCCTAGTTATGAGATCTTTGTAATCCTCTTATTAAGAACACTAGGCAAAGCTTTTTATAGTGTTAATCCCCTGATGCACTTCAGCATTGGTTCTAACCACTATACCCACTTTACTTCACCGATTAGAAGGTATATAGATCTAACCATTCACAGGTTGTTGTGAATGCATCTTTTTACTCCCGATCAATTCACTGATAATGAAAGAGATCAACTCAAACAAGAGTTGGAAAAAATTGCTGATACAGTTAATGATACAGAGATTAAAATTATCAATTGTGAAAGAAATGCCAATGATTATCTAACAACGCTGTTATTATCAAAACAAATTGGCAAAACCTTCAGCGGATTTATTTCAGCAATTACTAGCTTTGGAATTTTTATGAGAATGGATGAAAATAACTTTGATGGGTTAATCAAAATTACAACTATCCCTGATGTTTTCTTTATTTTTGAAAAGGAAAAAATGGTATTGAAAGGAAGAAAAACTAATAAGGTTTATAAAATTGGCGATCGTTTGGAAGCTAAACTAAGTGAGATTGATTTCATCCAAAAACGTGCTATTTTAACACTCATATAATTAGCCAATGGTTGTTAATATCCTGCTTTTTATTACCCTTATTTTTTTACTTTTACTGTTTGTTTTTTTAATTGCTTTTGCATTTCTAAACAAACGGGTTAGAAATTATGTTGTACGTACTTGAACTAGTGTTTTCAGTAAGTCCAAACAAAACCTTGATAAAAAGAACTTTTTTGACAATTTAACTTCAACTTTATTAAGACTTTCAGTTGATAAAATTGGGGCTATTATTGCCATTGAAAAACGTGATTCACTTGATCCTTATATCAACATTGGTTATCGGGTTAGTTCTGATTTTTCCCCTGAACTGTTAGTTACTATTTTTTACAACAAATCATCTCCTTTACATGATGGAGCGGTGATTGTTAGAGATTACAAAATTATTAGTGTCTCTTCTTACTTTCCAATGACAAGACAATTAATTGATGTTTCATATGGTTCAAGACACAGAAGTGCCTTGGGATTAAGTGAAAAGTCTGATGCAGTTGTTTTTATTGTTAGTGAAACAACTGGTAAAATATCAGTTGCTTTAAAAGGGGTTATTAAAACCTTGTCTTCCAACTCAGATAGGTTGCAAGATGAAATTATCCATTACTTGTCTTCAAAGTAGGATTAATCACTTTTCATTCTGGTTTTTTAAACCATGGATTCAAAGGGTTAATTCTTTGGTAAAAAAAGCGTCCCTGTAAGTGATCAAATTCATGCTGTAAACACATTCCAAATAGTCCAGTTGCTTTAATGGTAATCTCTTTTTTTTCAAACCAATCATAACCTTTAATAGTGATCCATTCACTACGAATTACATAACCTTTGTGCTGCTTTTTAACACTTAAACACCCCTCACCACTTTCTAAAAATGCTTTATTTTCACTTTGATCAATAATTTTAGGATTGATCAGTAGGCACTTTTTTTCTTTGTTTAAATCATTGAGGTGGATGTAAAACAGTTGTTTTCAATAGCCAATCTGGTTAGCAGCTATCCCAATTCCTGGAATAATGTCATATTCTTGTGCTTTACCATCATATGAAGCATCAACATATGCAATCATCTTTTTAATACAGGTTTCAATTTGCTCATCTATTGGAAAATTAACAGCTTCAGTTGGCTTGTTAATCAAAGCATTATCATCAAAAACAAGCCAAGTTTTAGTTGGTTGAAAAGTCATTGATAAAAACAGTAAAGTTAAAATTATTCTAACAATTGAAGTGAACAATCAGGGGAGAATTTTTGTCATTACTGGTCCTAGCGGTGTTGGCAAAAGCACCCTTGTTAAAGCCTTATTAGATCATTTCAAAGAACAACTGTTCTACAGTATCTCTGCAACTACAAGAAAAAAGCGCATTAGTGAAAAAGAGGGAATTGATTATTTTTTTAAAGATAAAGATGAGTTTGAAAACTTAATAAAACAAGATGCTTTCATTGAATGGGCTTGCTATAATAACCATTATTATGGAACGCTCAAGTCTCAAGCTGAACAAGCAATTAAAAGCGGAATTAATTTAATGCTTGAAATTGAGTATCAAGGTGCTTTACAGGTTAAAAGTAAATATCCTCATAACGTTGTTTTAATTTTCATTAAACCACCTTCAATGCAAGAGTTGTTAAAACGTTTAAAAAAGCGTAATGATGAAGATGAAACCACAATTAAAAAACGTTTAGAACAAGCTAAGATAGAGTTTCAACAGATTGATAATTTTAAGTATGTTGTCACTAACAAAGAGTTTGATAAAACCCTTAATGAGTTGAAATCAATCTTACTATCTGAGTTTATTTAATGCAAGCTGCTAATGATCATTTCTTTACCGGTTTATCAAAAAAAGGTCCTGTAAGAAAGGAAAATCAGGACTTTTATGGATTTAGTTTTAATCAAAATAACCTACTAATAGTTGTTTGTGATGGTCTTGGTGGTTATAAGGGTGGCAAGATTGCTAGTAACTTAGTAGGTAAACTCTTTCTTAGTTTATTTGAAGGTTTTGAATTTAACCAGTGAGATGAAACTACAGTTAAAAAGTGGTTTGAAAATACTTTAATACAAGCACGCTTTCAACTAGAAAATTGTTTTCAAACAGTTTATGAAGCACAAATCCAGTTTGCTAGGATGGCTAGCACCTTGGTCTTGGGAATTTTAACTAAAAGTGATATTTATATCTTTTGAATTGGTGATTCCAGAGCTTACTTATTATTTGAAAACCAAGCTAAGTTAGTTACTAAGGATCACAATCTTTATAACCAGTTAGTTGCTATGAATGCTGATGAGAAATTACTTTTAAGTTATTCTAATCAACTTTTAGCATTAACAAATACTATTTCCAAAGAAACAAAAAGACCATTAGTTTATGGTTTTTATAACACAAAAATTGAACAACAAGAATTTTTATTATTGTGTTCTGATGGACTTTATAACTTTGTTGAAAAAGAGCTTTTTTTTGAAATTATCACTAATTCTAAAAACCTAAAACAAGCAGTGTTTAATCTTTATAGAAAAAGTATTGAAAATGCAAGTAATGATAATATTACTGCTGCCCTTGTTAATCTGCAAAAATGGAAGCAATCTTAAAGATAGGTGATATTGTTGAAAATAAGTATCAAATTGAAAAGCTTTTAAATAGGGGTGGGATGGATTCCTATCTTTTTTTAGCAAAAAATTTGAACTTAAAAAACTATGGACCAGTTCAAAAAAAACAGTATGGACACTTAGTTTTAAAGGTGGTTCAAAAAAATCCTAAGATTAATGAAAATAATTGGAAAAAGTTCCTTGATGAAATGGTAACCACAACTAGAGTGCACCATTCTAACCTAGTGAAAAGTTTTGATGTGGTGAACCCTTTTTTGAAAATAGTTCGTGGTAATAAAACAATTGCTCTCAATCAGATAGTGATGATTGCAATGGAGTATGTTGATGGCCCTTCTTTAAGACAATTGCTTAACAGAAAAGGCTACTTTAGTGTTAGCGAAGTAGTTTATTACTTTACAAAGATAGTAAAAGCCATTGATTATCTCCACAGTTTCAAGCACCAAATTATCCACCGTGATCTTAAACCTGAAAACATCTTATTTACTAGTGATTTAACTGATATTAAACTATTAGATTTTGGCATTGCTTCAACAGTAGTTAAAGTTGCTGAAAAAACCGAAGTTTTAACTGATGAAAATTCACTGTTTGGCACAGTTAGTTATATGATTCCTGATGTTTTGGAAAGCACAGTTAATAAAGCTGGTAAAAAGGTTAGAAAACCACCCAATGCCCAGTATGATATCTACTCGTTAGGAATTATTCTATTTGAGATGTTAGTTGGTAGAGTTCCATTTAACAAGTCAATTAACCCCAACAAGGAAAGAGAAACAATCCAAAAAGCACGCAATTTTGACTTACCTTTAATGCAAGCAACTAGAAGTGATATCCCAAATAGCTTAGAAAATATTGCTTTTCGTTGTACTGCTGTGAAAAGAGAAAACAATAAGTGACTTTACAGTTCCACAAAAGAACTTTTAGAAGATTTGGCGAATTGAGAAAATGAACAAGCGATGATTAAACCTGCTAATGAAAGGGTTTTAGAAGGGCAGGTGGAAATTAGAGAAATGATGCTTGAAAAGCCCTTAGCTTGGTATTTCAAAACTTGAGCCTTATCAATCTTTACTATCGTTTTTATAGGGTTAATTATTGCTGCAATAGTTCTTTTATTAATTTTTAATGCCAGATTCTAACTTTGGGATAGTGCTTCAGTCATTAGCAAAACAATGCAAAGTATTTTGGAATAACCAGATAATAACTGCTTTTCCTCAAAAGAAGTTACAGTGAAAAAATGATTTTAAGTTGATGGTTGGTGATAGGGTTCAACTTGAAGATGGTGCGATCACTAAAGTTTTAGCTAGAAAAAATGAACTTACAAGACCAAGGGTTGCTAATGTTGATCAGATTGTTTTAATCCAATCACTAGTTCAACCTAAGATTAACTGAATTCAACTGTTGAAATTATTGGTATATTTCAATGCTAAATTAATTGATGAAATACCTATTTTAATAACAAAAACAGACCTTGATTTTGATCCAATGGAAAAACAAAAGTTAATCGATTTAAAACAGTTTAACTATCAGTTATTTTTTGTTTCTAAAAATGAACCACTACCTTCAGAATTAATCGATATTTTTAGTAAAAAACTAAGTGTTTTTACAGGTCAATCTGGTGTTGGTAAATCTAGCTTAATTAATCGTTTAGATCCTTCTTTAAAACAAAAGATTCAAGCCTTATCAGTTAATAAATTTGGTAAGAATACCACTACTAAAACAACACTTTTTTCATTTAGAGGCGGTTTTATTTGTGACACCCCTGGTTTTAATGTAATTTCTATTAAAAACCTCAAAATTTTAGCAGCCCAACACTTTGTTGGTTTTCAGAAAATGATTAGTAAGTGTCATTTTTCTAACTGTTATCATCAGTATGAAAAAGATTGCTTTGTAACCACAAGTGTTATGAAAAACAGATATCCTTCGTGATTGTATGAGAAGTATAGAAAAATGATTAATTAAAATCAACTGCAAAAGTGTTTATGAGTGATAAATTATTAACAATTGACTTAAGTCATGTTTATGGATTTGATAAAGAAATTATTTTTAAGAAATACCAAAAAAAAGTAGATCAAATTCACCAAGATTTTCTAGCTCATAAACTTGCTGATGGTCACATGACTGGGTGGTATGACCAACCTGATCAAAACCACCAATTCCTTTTAAAAACCATTAATCAAATTGACAAAAAGTTTAAAAGTTTAAAAGTAACTGACATTGTTTATGTTGGTATTGGTGGTTCTTTTACTGGTATTAAAACAGTTTTAGATTTCTTAAAACCAAAACAAAGAACAGGATTAAAAATCCACTTTGTCCCTGACCTTTCTGCTTTTCAAGCTGCAAGTGTTATTAAGGAAATTAAAAATAAATCATGGGCTCTAATTACCACTTCTAAGTCTGGTAGAACCCTAGAACCAGCACTGAATTTCCGCATTTTTAGAAACTTATTAAACAAGCGTTATGGCAACAAACACTACCAAAGAGTAGTTGTTATTACTGATGAAAAAAAGGGATTACTAACCAAAATGGCATCAAATCATGGTTACCAAAAGTTAGTTATTGATTCAAATATCGGTGGGCGTTTTTCAACTCTATCTCCTGCTGGTTTGTTACTAGCCAAACTTTTTGGTCATGATCCTAAGGCCATCTTAAAAGGAACATTACAAGCCAAAAAGGATTTGCAAACAACTTCACTTGAAAACAATTCTGCATACCTTTATGCAGTAGTTAGACATTGACTATACACAACAAAAAAATTCAAAATTGAAGTTTGCATTGCTTATCACAGTTTGTATGAATATTTGTTATTACAGCATCGACAACTTTTTGGTGAATCAGAAGGTAAGAACGATAAATCTTTATTTCCTACTTTTTCGATTTTTACTGTTGACTTACACTCAATGGGACAACTCTATCAAGAAGGGGAAAAAGTGTTTTTTGAAACAGTAATTGATGTTAAAAATCCACTTGTTAATATTAATTTACCTCCATCTGATTTTGACAATGATGATGAACTTGATTTCTTGTTAGATAAAAGCTTAAATGAGATTTCAGATGTTGCAATTGATTCAGTTATTAAAGCGCACTACCAAGCAAATGTAAGCATTATTAAATTAACTTTAAAAGAACAATCTGCATTTATGTTTGGTTATTTTTACTTTTGACTCTCTGTTGCTACAGTGATGAGTGGATCATTATTAGGGCATAATGTCTTTAATCAACCTGGCGTTGAAGTTTATAAAAAGTTAATGTTTGAAAAACTAAGAAGTGGCCACTAAGGTTGTTTTTTCACTCTTACCACTTTTAAATAGGTTTGACAAGTCACTTTTAGAAAGTTACTTTCAAGATGGATTGAGGTTAATCCATTATGATGTGATGGACCAATTTGTTCATAATACTGCTTTTAAAGGTGAATATTTGGATGAATTGAAAACAATAGGTTTTGATGTTAATGTCCATTTAATGGTGGAACAGATCATCCCTCAAATAAATTTTTATCTTTCACAACCTAATGTGAAAAGGATTTCGTTTCATGTTGAACCATTTAGTTTTGCAAAGATTAAAGAACTAATCCAACTAGTTAAAGAAAATGGTAAAGAAGTTGGTCTTGCTTTTAAATTTACAACCAATTTACAACTATACCAACCATTTTTTACAACCATCGACTTTATCACTTTAATGAGTGTTCCTCCTGGTAAAGGTGGTCAAGCTTTTAACGAAGCTGTTTTTACAAATTTAAAGATTGCTAACCATTACAACTTGAAAATTGAGATTGATGGTGGGATTAAAGTTAATAACATTGATCAAATTAAAGCCTTTGTTGATTTCATTGTAATGGGAAGTGGCTTTATAAAATTAGAGCAGTGGCAACGTCAAAAATTGTTGCAAACAATCTAATTAAACTTTATTGATGAAATCAGTTACAGTCAAGCAATTACTACAAACCCCACGAAAATTTAATAACAAGCAGATTAAACTATCAGGTTGGGTTAAAAATAAACGTGCTAGTGCTAACATCATCTTTCTAGCAATTAGTGATGGCTCTAGTATTAATACCCTACAAGCAGTAGTAAAACAAGAAGATAACCCCCAGGTTTTCTCACTGTTACAAACTGTTAATTTAACAAGTGCTGTTATGGTTTGAGGGGAAATTATCTTAACCCCAAAAGCTAAACAACCACTGGAGTTGAAATTAAAGCAGGTGAGTTTATTAGCACAAGCAGAGTCTGATTATCCACTGCAAAAAAAAGAACATAGTCAAGAGTTTTTTAGAAGTAATGCGCATCTAAGAGTAAGAGCAAAAACTTACTTTGCAGTGATGAAAATAAGGAGTGTTTTGTCACACGCAATCTTTGAATACTTCTTTAAAAATGATTTTATCTTAGTGCAAAGCCCTATTTTAACTAGTAATGATTGTGAGGGAGCGGGGGAAACATTTGTAATTAAAGATAGTGAAACTTTTTTTAATAAAACGACTTTTTTAACAGTAAGTGGCCAGTTTGGAGCAGAAGCTTTTGCGCAAGCATTTAAAAAGGTTTTCACCTTTGGTCCTACTTTCAGAGCTGAAAAATCCCATACTAATCGTCATCTTAGTGAGTTTTGGATGATCGAACCTGAAATTGCATTTGCTAACTTAAAAGATTTAATGCAGTTAATACAAAACCTAATTAAATTCTTAATTAAAAAAGTGATGGAAAATGCTAGTGATGAACTAAATGTTTTAGCAAAGCAATTTAGCAATGACATTATTAGCAACTTAAAGACAATCATTAGTACTAAAAAATTTCCAATCATTGAATACAGCAAAGCATTAGCGATTCTAAAGGAATCTAGTGATACAAAAAAAACTAATTTTGAACTAAACGACTTTAGTTTTGGTATTGACTTAAAAACAGAACATGAACGCTTTTTGTGCGAACAATATTTTCAAAATCAACCGCTTTTTGTTATTAACTATCCAAAGGAGTTAAAGGCATTTTACATGAAAACAAATACTGACAATAAAACTGTTGCTGCAGTTGATCTTTTATTACCAAAGATTGGTGAGATTTGTGGGGGAAGTGAAAGGGAAAGTGATTTAAACCAACTTAAGAATAGGTGTCAATCTTTAAACATTGACACAAAAAGTTTGAACTGATATCTTGATATGAGGAAATGGGGTTATTTTGCTAGTGCAGGTTTTGGTTTGGGCTTTGATAGATTATTAGCTTATATATGTGGATTGGAAAACATCAGAGATGCTATTCCCTTTCCCCGTGTACATGGCACCATTAACTTCTAAATTCGCTGCTTATAAAAAAAAGATTGCAAACTGGTTAACAGTTTACAGAATTTTTATTGCTTTACCTACTATTATTTTTATTGCTTTAGATAATCAACTAGGAGTTTTAGCTAACTTTTCTGTTGGTGCAATTAGCATTAGTTTACAGATCAGTTTATTGATTGGAGGATTTTTGTTTTTAACTGCAGTTATATCAGATTATTTAGATGGATATTTAGCAAGAAAATGGCTAGCAGTTTCTAACTTTGGTAAATTATGAGACCCCATTGCTGATAAAGTGATTATCAATGGTGTTCTTATTGCACTAGCGATTAATGGATATTTTCACTTTAGCTTATTAATTGTTTTTATAGTCCGTGATCTTGTGTTGGATGGAATGCGGATTTATGCTTATGAGAAAAAGGTGGTTATTGCTGCTAACTGACTTGGAAAATGAAAAACTATCATGCAGATGGTTGGTATTGTTTTTAGTTGTTTTGTTTGGAGTTTTAAACAAAGTGAAATAGCTTCTTTGAATAGTGGACTGTTCTTTTGATTACTAACTCAACTGCCATATTATTTAGCAGCAGTTTTTTCAATTTGGTCTTTCATTGTTTATAACATCCAAATATATCAGCAACTAAAGGCTTATAACTCCAAGTTATAATCCTTATTAGGCATTGGAATAACTTTGTTTGCCAATTTAATAGCAGAAAAACTCCAAAAGTTACAACTTAGTGTTGCAACAGCTGAATCAGTTACTGGTGGCTTATTAGCTCATTGTTTAACTTCCATTGATGGTGCTTCTAACTATTTTAATGGCGGTGTTATTGCTTACAATAACCAAGTTAAGATTAACTTACTGAATGTTCAATCCTCCACAATTGCAAACCATGGGGCAGTTTCCAGTTTCTGTGCTAGGGAGATGGCAGTTGGGGTTAAACAAAAGTTTCAAGCTGATGTGGGTATTGCTTGCAGTGGGATAGCAGGTAGTAAAGCAGTTGAAAATAAAGCAATAGGATTACTTTTTTTCTGTATTATTATAGGAAATAAGGCTTATGATTTTGAGTTTGAAATGAACCAAAATAATCGTAAGGATAACATTGAATTATTTACCAATAAGATCTTGGAATCTTTCCACTATTTGTTAACAAAGCTAGCTTAATCTAAGTTATGAAACGTACTTTAAATATAGGTATTGTTTTGTGTGAAAATTTTCTTTCAGACCAACAAAATGCTGTTGATAGTTACACCCAAGTTTATGAAGATGTTAGGATGTTTGAATTTGGGTTAAAACTCTTTCAATCATTACCTTTTAACATCCAAGAAACCCTTATATTTTGTAATGCTGAACAACATAAAATAGTTGATAAAGCTGCTAAAAAATACAAAAACACTACTGTGTTTTTTTCACGTGATACTGATGTAGCTAATGTTTATGAAGCTAAGGTATTTATCCAAGAAAAATACAAATTAACCCAAGATTACAAAAAAAGAGGTGTTAGCTCTTATTATGACAGCTGTTGCTTTATCTTAATTGAAGCGAATCGTCCATTAACTGCAATAAAGACAGTTAAAAGTGTTTATGAAAAAGCACTGATAGAAAAAGCAGCTATTGCAGTATTACCATATAATGGTACACTAATGAATGGTAACAATGATGTTGTCTTTAGCCACTTGCAAGATAAAAATAGGTTTAACTATTGAAAACAAAGCAAGGCTTATGAAGTACAATACCCCCAAGCATATACTTTAAATAAACTTAATCAGTTTTCAAAACAGCAATTTCTAAGGGCTAGAAGTATGTTGGACTTAATGAAGATTTCTAATAAATCACCCTTAAGTATTGTTGATGGTAGTGCTTATGCTTTTAGGGTTGTTACCAACCTTGATTTTGAAATTTTATTAGGTATTTTAAAAAATGGATAAAAATATTTTTCAATTAGTACAAAGTTTTGCTAAAACGCAAAATGTGCGAGCTAATTACACCTCACAAGACAAAAATATTAGTTTAGATTTTGTTAGTTTTGAAACAGTTAGTCAATCACTAACAGGTTTTCTAATTTTTAATAATTTCAATAAATTGTTACAACTAATTGAACTGATAAAACAAAAGCAAACATGATTATATGTAGATCAGCTCTGAATTGTTGATCTTTCCAATAACAAAGCCTTAACTGATGCTACTGTTTGGATTATTAAACAAGAAAAATTGCCTGTTTCAGTTGCGGCTTTTAGCAACCAACAACTCAACCAAGTTTATCGCAATTCATCCACAAGTCCTTTGTATCTTAGTTATGTCAAACCAATCGAAGTTCAACAATTTTTTACACTTTCTCCATCCATTAACAATAATCCTTTTTTAAATCAAAATCCTTTAACTGAATCACCATTTGATAACAATAATCAGCTGTTTCAAGCAACAAAGAGCGTTGAACCTTCAATGGAAACAATGGAATTTTCCCGGTTTATGGATGAATTTGATCAGATTACAAAGAACTTTTCTGATATAGAGCTTGAACCTATGCAATTCACCCAAAGTTTTGATGATTGGAGCAAAGACTAGGGTTGCAATAGTTGGCGGGATTGGTTACATAGGTAGTTGTTTTGCTAGTTTTATCAAAGAACAAAATGATAAGCTAATTGTTACTGTTATTGATAACAACAAAAATAACCATGTAATTAAACTCTTAAAAAAGATTGGAATTGAATTCTATTTTGCTGATTTACTAGATAGACATAAGCTAACTGAAGTAATTGCAGCAATTCAACCTGATGTGGTATTTCACTTTGCTGCTAAAACAAGTGTAAGTGAATCAGTACATAATCCATTGAAGTACTTTGATTGCAATGTAATTGGTACTTTAAACCTAATTAGTGCAATTAGTAACTTACAGAAGCCAATTAAATTATTTTTCGCTTCTAGTGCTGCAGTGTATGGTCAAACAACTAATAGTTACATTAGTGAAGAGATTGTAATAACTGAAACACAAGCAACCAATCCTTATGGATTGAGTAAGTTTTTAGATGAATTAATCTTAAATGCAGTTGCCAAAAATAGTCAACTACAAGTTGTTTGCTTACGCTTTTTTAATGTGGCAGGTGCAATTCTGCCATTTGGTAATTTTAATGGTAATACCACGCTTTTAATTCCTAACTTAGTAAAAGCCTTTTTAAAACAAACTCCCTTTTTTTTATATGGCAATGATTATGCAACTAAGGATGGTAGTTGCATAAGAGATTACATCCATGTTTATGATATATGTAATGCTCATTTCTTATTATGAAAGTGGTTAAATGATCATCGCCAAATTAAATTTGAAACCTTTAACTTGGGGAGTGGGATAGGAACTTCTAATTTAGAAGTTATTGATATTGCTAAAAAAGTGTTTTATCCTAGTAGATTAAATTTAGAAATTAGACCAAAAAGAAGCGGAGATCCAGCAATTTTAGTAGCAAATGTTGCTAAAGCAAAACAAACCTTTCAATTCAAAATAACGCGTAATTTGAAAGATATGATAAGTGATGAGCGTAATTTTTATGAGAATTTTTATAATGACGCTTATTAAGTGGTGTTTAATTAATGGAAAAAGTTGCCTTCAAAATGGAGCATATCTCCAAAAGTTTTGACAATGGCAAAATTAAGGCTAATGTTGATGTTAGCTTAGTTGTTTATGAAAATACTGTCCACACCATTTTGGGGGAGAATGGTGCAGGAAAATCAACCCTGACTTCGATTTTATTTGGTTTATATAAACCTGATAGTGGCAAGATCTTTATTGGTGAAAAGCAAGTAAATTTTAAATCTTCTAAAGATGCAGTAAAACATAAAATCGGAATGGTGCACCAGCACTTTAAGTTAATAGAAAACTACACGGTTTTAGATAACATCATTCTAGGGAATGAAAGTAGGTTTGGGTTTTTACCTTTAATTAATCGTAAAGTAAGTGAAGCAAAGATTAAAACCATCATGGAAAAATATGGAATCTTTGTTGATCTTAAACAAAAAGTTAGTAACTTAACAGTAAGTCAGCAACAACGGGTTGAGATCCTAAAGGTTTTATTTCGTGATAGTAATATCCTCATCTTTGATGAACCCACTGCAGTTTTAAGTGATCTTGAAATTCAAAACTTTCTCAAGATTATTGCTAACTTTAAAAAGCTAGGAAAAACAATTGTTTTAATCTCTCATAAATTAAATGAAATTAAACAAGTTGCTGATACAGCTACTGTCTTAAGACTTGGCAAGGTAGTTGGTAGATTTGATGTTAAAACAACACCAGTTGATAAGATTGCGCTTTTAATGATGGGCAAAGAGTTAAAACAAACTAAAAACACCACAGATTTTGTTGCTAAAGATGAACCTGTTTTAAAAGTTCAAAACCTGAATTTGTTTCTCAATAAATCTTTAGCATACAAGTTCTTAGTGAGATGCAATAACATCCATAAAGCCCAACAAATTAAGAAAAATAAACCATTAAAAGACTTATGGATAATTAGTTTTTTAAATAAACTAACCACCAGTAACAAAACCCCTAAATTAGTAAAAGGCTTGATTAATAAGTTAGGACTTTCCTATCAAGAAAATACAGATGAAACCATTAGTTTTGCTATCCATAAGGGAGAAATTTTTGCTATTGCTGGGGTTGAGGGTAATGGTCAAAGTCAGCTTGTTAATTTAATTTGTGGAATTGAAAAAGCTGCTAGTAATAAGTTAATTTTTAACAATATTGATATCTCAAGATGATCAATTAGAAAACGGATTAATGCTGGGATTAGTTTTGTTTTGGAAGATAGACATAAATATGGCTTGATCTTAGATCAAGCCGTGAGGTTTAATACGGTTAATAACCAGATTAATAACCGTCCTTTTAGTAGTTGAAACTTTTTAAAACCAATGGAGATTGCTCTTTATAGCAACACTATTATTAAAAAGTTTGATGTTAGGGGCAGTGCTGAGGGTAGTGCTGTTGTAAGAAGACTTTCAGGTGGTAATCAACAGAAACTAATTATTGGTCGAGAAATGACCAAACAAAATGACCTTTTGGTGTTAGCACAAGTAACCAGAGGCCTTGATATTGGTGCTATTGCTTTTATCCATGAAAACATCTTATTAGCTAAAGCTAATAATAAAGCTATCTTATTGGTTTCATATGAACTTGATGAGATCTTAGCACTTGCTGATACAGTGGCTGTTATCAATAAGGGGAGAATAGTTGGTATGGGAAAAAGAGATTTAATGGATCGCCAATCGATAGGTAGATTAATAATGCAATAAAAGACTATGACAATGTGGCAATTTAAAAGTTACTTTAAACACCACCTGGTGTTTTGAAAAGACCGATTTTTATATAGCTCTGAGAAACAAATGCAAAGAAGAAGTATCCTCTCTTCAGTGGTTTTGATAATCCTCTCTTTTCTTATATCGTTTTTACTGATTATTTCAATTCCTGGAGGTAGAGGTGCGAGCTTCTTTGCACTGTTTACTAAGTTATTTTTAGATAACACTAATACTGAAAATTTCTTAAGACAGATTGCTATTTATATCCTAGCTGGATTAGCATTTAGTTTCTGTATGAGTGTTGGTATTTTCAACATTGGTATCTCAGGGCAGATGATGGCTGGAGCCATCTTTGGGTTTTTAATGATTCTCAAGGTGTTTCCAAGTTCATTTCGACCTGGTTTTGGAGGTCAGATTATTACTGTATTATTGATGGTAATAGGTAGTGTTAGTGTGGCAGTTGTTGTTGCAACTTTAAAGATTTTCTTCAAGGTTAATGAAGTTGTAAGTGCAATTATGTTGAACTGAATTGTAGTGCTTATTAGTGCTTATTTAGTAGAGACTTACATTAAAAATAATAGTGGGGGTACAGCCCAATTCTTTTCCTTACCACTCCCTGATGAATTTGCTTTATATAACTTCTCTCCTTTAACAAAAAAGTTTGGTTGATTAGCTTCACTTATTATTGCTTTCATTAGTGTTATTATTGTGGCAGTAGTATTAAAATACACAGTTTTTGGACACAAATTAAAGTCAATTGGTAGTAGTGTATTTGGTTCTCAGGCAATGGGTTTTAATGTTAAAAAATACCAGTTCTTATCGTTTATTATCTCAGGAATTTTATCAGGACTATTAGCAACGGTTGTTTACACTGCATCAACTGAAAAAGTATTGACATTTAACAATGTTGGGGATAGTGCTATTTCAGCAGTACCAGCTACTGGTTTTGATGGGATTGCGATTGGTTTAATTGCTTTAAATAACCCCTTTAGGATTGTTATTGTTTCTGTTCTTATTGCTTTTGTTAACATTGGGGCAAGACCTGCTAATTTAAACCCTAGTACTGCTAGTTTAGTTTTAGGAATCATGATGTATTTTGCTGCACTTTATAACCTAATGGTTTACTTTAAACCATGAAGATACCTAGTGAAGCTGAACATTGGAAAGATAAATCTCACCACATATGAAACATATGAAAACAAACTAGCTGCTAACCTAGAGTGACTAAGTTTCCAACGCTTCTTGTCAAAACAGAAAAAAAAGAATGACAAAACTAAATTTAATTGGTTTGATACTAGTTTATTTGAACAATATGCAAAAAACAAACAAGAAATTGTTCAAGAATACCATCACAATTGTGCAACTAATTTAATTGCTTGGTGATTGAATGCAATCAAAAGTGGCAATATTAAACCTTCAACTACTTTTAAGTTGGAATTTGTTAATTTTAAACACCAACAGAAGTTTGTATTAAATTGGTTTAAAAATGAAAGTGAATCACTGCGTGATTTCCAATCACAGTTTGAGAGAATCAATAAGTTAGTGGAAAGGGAGTTTGTTAAGTAATAATGTTAAGTTTAGCACAATTAGAAAGTTGGTTTTTTATCGCTCCAGCACTGCTTTTAGCAGTATTGAGTGGTTATCTTGCTGAACGCGTTGGGATCATTAATATTGCTATTAATGGTGGAATGGTGTTTGGTGGGTTATTTATGGCACTCTTATCGTATGGATTTACAAATAACTTAAACCAATCCGCTCCCTCCTGATCACTATTTATCACCATTCCCTTATCAGTTTTATTTAGTAGTGTTATAGGTTGTTTATTTGCACTAGCAACAGTTAAGTTAAGAGCAGATCATGTTATTGTGGGAACTGGGATTAACTTGTTGGCTAGCGGAATTACCCTTTTTATTAGTCAGAATGCTGCTAGTTTGTTTTCCGATACTACCTTAAGAGTAAGGTACTTATTTCCCATCCAAACTACTGTTAGTATAGAAGCAATTGGTGTGTTTGTTTTTAGTTTACTTCTGATTGGGTTTGTATGGTACTTGATGAGTTTTACTAAAACTGGGTTGAGATACCGTGCGGTAGGTGAGAATCCTAATGTAATTGATACCCAAGGGATTAGTGTTTACAAATACCAATGGATAGGCGCAATTTGTTCAATGATGGTAGCTGGATTGAGTGGTAGTTTGTTTGTTTTAAGTGTTTCTAACTTTCCCTTTAACAGCGGAGATGTAAATGGCTTGGGTTTTATTGCTATTGCCATTATGATTATCTCAATGTGAAGAATTATCCCTAGCATCTTTATTGGGTTAATCTTTGCATATGCCTATGTTTTTACCAATAGTCAAATAGGATCTAATAGTAATTCCTACTTGTTAAGAACGATCCCTTTCATCATCTCATTACTAGTAATGTTGTTATTTGGTTTTCTTAATGTTGCCCCAAAAAATATAGGTAAACATTTTGACAAGGGTTTAAGATAAACAAAAACCTTATTTATAGTTAAGTAAGTAGTTTTATTAATGATTAAAAACCTGGTGGTGATTGAATCACCCAATAAAGTTAAAACATTAAAACAATATCTTCCTAGTGATGAATTTGAGATAGTCTCAACCGTTGGTCACATCAGAGAAATGGTGTATAAAAACTTTGGTTTTGATGAAAATACCTATACCCCTATCTGAGAAGATTGAACTAAAAATAAACAGAAAAATCCCAAACAGAAACACCTGCTCAGTAAGTTTGAGATCATCAAATCAATCAAAGCTAAAGCTAGTGATGCACAAAACATTTTTTTAGCTTCTGACCCTGATAGAGAAGGGGAAGCCATCTCTTGGCATGTCTATGATTTATTGGATCAAAAAGATAAAGCTAAGTGCAAACGAATCACTTTCAATGAGATCACTAAAAAAGCAGTAGTAGATGCATTAAAACAACCGCGTAACATCGATCTTAACTGGGTTGAAAGTCAGTTTGCCCGCCAAATCCTTGACAGGATGATTGGTTTTAGATTATCAAGATTATTAAATAGTTATCTGCAAGCAAAGTCTGCAGGTAGAGTTCAATCAGTGGCTTTGCGCTTTCTTGAGGAAAGAGAAAAGGAGATAGCTAAGTTTGTTCCGCGTTTTTGGTGGACAGTTGATGTTTTATTAAACAAAGAAAATAACCAAAAAGTAGTTTGTGCAAACAAGTCTATTCCTTTGGTTTTAAGAGAAATTAACCCTGAATTAAGTGCTAGTTTAAAACTGGATTTTGAAGCTGCTGAAAACGTATCAGGAATTGACTTTTTAAATGAAGCTTCAGCAACCAGATTTGCCAACCAACTGACTGGCGAATATGAAGTTTATTTTATTGATGAACCTAAGATTTACTATTCATCTCCAAACCCAGTTTATACCACCGCTTCACTTCAAAAGGATGCAATTAATAAGTTAGGATGGTCTTCCAAAAAAGTAACAATGGTGGCCCAAAGACTGTATGAAGGGATTAGTGTTAATGGGAAACAAACTGCATTAATTAGTTATCCAAGAACTGATTCAATTAGGATTTCAAACCAATTTCAATCAGAGTGTGAAAAGTACATTGAAAAGGAGTTTGGAAGTCATTATTTAGCTGATAAAAATAAGTTAAAAAGACATAAAAAGGATGAGAAAATCATCCAAGATGCCCATGAAGGGATCCATCCTACTTACATTACTATTACCCCCAATGATCTGAAAAACGGGGTGAAACGCGATGAGTTTCTCCTTTATCGTTTAATATGGATTAGAACAGTTGCTAGTTTAATGGCAGATGCTAAAACATCAAGAACTATTGTTCGTTTTATAAACCAAAAAAACAAGTTTTATACCTCTTCAAAATCACTTTTATTTGATGGTTATCAAAGGTTATATGAAGAGATTAAACCTAATACTAAAGATGAACTTTACATTGATCTTAGTAAGCTTAAAATTGGTGATAAATTTAGTTTTGAAAAGATCAGCGTTAATGAGCATAAAACCAACCCACCACCACGTTACACCCAAGCTAGTTTAATTGAAGAGCTTGAAAAATCTAACATCGGTCGTCCCTCTACTTATAACACTATGGCCAGTGTTAATCTAGAAAGGGGCTATGCTAACTTAGTGAACCGATTTTTTTATATCACTGAGCTTGGTGAAAAAGTTAATAATGAACTTTCCAAGCATTTTGGGAATGTAATTAATAAAGAATTTACCAAGAAGATGGAAAAATCTTTGGATGAAATTGCTGAAAACAAAGTAAACTATCAAGAATTTCTTAAGCAGTTTTGAACAAATTTTAAATCTGATGTTAAACTAGCTGAAAATTCAATTCAAAAAGTGAAAAAGGAAAAGAATTGGTTGAAAGAGATTGTCCTAAATGTAATCAACCGTTGGTATATCGTTACACCAAAAGAGGTAATGAGAAGTTTGTTGGTTGTAGTGATTTTCCTAAGTGTAAATACAGTGAGTTTAGTAATCCTAAACCAAAACTAACCTTGGAAACACTTGATGAATTGTGTCCTGAGTGTAACAATAAACTGGTTAAGAGGAGAACTAAATTTAACGCTAAAAAGACCTTTATAGGTTGCAGTAATTTCCCTAACTGCCGTTTTATCAAAAAGGATAATGCTGCTGAATTTAAACAATAACAGCGTTCTTATTGTTGCGTTTGTAATTGTTTCTTTATTCTTTCTAATAATTGTTGGGTTTGCTTTAAATTTAGCAATTGCTTTTTCACTCCATTTAAAGCAGAATAAAAATAACAAAAAATACATCTTAAATGACCAACAGATCCAGTTAAGATTAACTGAAAAACAAGCCCAATTAACAACTTTACTTAACTTTTATCAACAAAAAATTGAAAGTGTAAACAGAGAAAAAAGTTGGTTAGAAAGTCAGTTACAGGTAATTGATAAAAAGGATTTAAAGCAAGCGCAAAAGTTAACTTTACATTTAAAAAAAGACCAAATATTAGCTCAACTTAATGAAAAGCTGATTCAAAAAAAAGTTGATCAGCCTTTAGTTAATGAACTACAGAAAATCAAACTTTCCTATCTTGAAAGGTTAGTTGATCAAAAGATTAAACTCAGTGAAAATAATTTCAAAAGTGCTTTTCTTAAAACGAAGGTAAAAGAGACAGCATTTAATATCTTTGCAGCTAAAAACAAGGTTAACTGGGAGTATTTTAAACAGGTGTGTGATGCTGATTGCACTTTAAAAAACTTAGAAGATGAAGTGGAAATTACTTTTTCTAATTGGAGTTATTTGAGAAGGATGCAAGCTCTATTAGCGTTTGAAAAACTAATTAGCAAAATCAAAACAGTCAAAATTAATGAATTGGTTATTAATGAAACTTTAGATGAAGTGAAAAACGAAATTAGCCAAACTGCTTTTCAAGCGGGTGAAAAAATAGTTAAGGAATTTCAGATAACTAATTTAAACGAGCAAATTACCAGACTAATTGGCTTGCAAAAATACTATTTTGGCACTGATCAGCTCAATCTCTTAGAACTCGCGGTATTAACAACCAAATTAGTAATTTTACTGAACAAAAAGTTCAAACTAGATTTAGATTTAGAACTTCTTAAAGCAGCTAGCTTGTTTAACTATTTAAAGTGAGTAGATAATAACCAGTTCTTTCAAACATTAAACACAAAACTTAACCAGCTTTTAATTAGTGATCAAGTTATTGCAATCATCCAACAACAAGAACTTAGTTTTTATCCTGATCAGTATGGGATGTTAATTAATGGAGTGAAAACAATGATTAGAGAGCATAACACCATTGATTTTGAAAAACTGGTTTTTCTCAACTCAACAAAACTTATTGATAATTTTTATCTCTATGATTTAAATATGATCCATGCAGTTGAATATAACAACTGCTTTTATTACTTTGTTAGTGTTAAACCCTTTGAGATTAAGTCATTAGCAGAACTTGATCTTTTTGTTGTTTTGTTAAAAACTTTTTTAGCTAAAAAGCAAAAGCAAAATCCAAAAGCGGTTAAACTGTTTATCACTACCAAAATTTTAGCGATCTAATTACATTAGATTTTATTAGAATTGTTTCAACTAATAAATTGGCCTTATGGTAACAGAAATTAGAAGTCTTAAACAACTTGAAGAGATCTTTTCAGCTAAGAAAAATGTTATTGTTGACTTTTGAGCAGCATGATGTGGTCCTTGTAAACTAACCAGCCCTGAGTTTCAAAAAGCAGCAGATGAATTTAGTGATGCTCAGTTTGTTAAGGTTAATGTTGATGATCATACTGATATAGCAGCAGCTTATAACATTACCTCTTTACCAACTATTGTTGTTTTTGAAAACGGGGTTGAAAAAAAGAGAGCCATTGGCTTTATGCCAAAAACCAAAATTATTGATCTTTTCAATAACTAAAGTCCATGATTGATCTGCTTGGTTTGGATCTGGATGGAACGTTATTATCTAAAACTAAAAAAATTAACAATCCATCAAAATTAGCATTAACTAATTTAATTGCTAAAAAACCAAGTTTAAAGGTGATGATTTTAACTGGTAGATCAGTTTTTTCTACTCTAAAACACGTTGAAAAGCTGAACAGTTTGTTTAAAAAACCAATTGTTGATTATTTTTGTTGTTATGGGGGTGCTAAACTTTATCAAATTGAAGCAAATAAGCCACAAGAAAGATACAAGTTTTGCTTGGAAAACAGTGTTGTTGAAACTACCTTTAGTATTATCAAAAAACACCGCGGATTATGTTTAGCTTACTTAGATAGTTATGTCTCTCCTTACCTTTGTTTAGCTGGTAACAAGCTCCTTGGGTGGTTCACTAAATACTTTTGGTATAGAAAAAGGTGTGTGTTTTTTAACCAGAACCATTTAAAACAAGGTATTTTAAAGATTAGTGTTTACTTTTTAAGTGCAAAAAGGTGTAAAAAAGTTTATGAAATCTTAAAAAATACCTTTCAAGAAAAGGTTAATGTTTTAAGTTTTTCTAATAATTTAATTGAGATAACTCATCATGATGCTAATAAGGGTTATGCAATTGAATATATGGCCAAAAGAGAACAACTTTCACTTAATAGAATAGCAGTTATTGGTGATTCTTGAAATGATTATGCAATGTTCAAAAAAGCTAAATATTCCTTTGCAATGTCAAAATCCCCTTCCCAGTTAAAATTAATTGCTACCAATACCAGTAACAAAACCAACCGTTACCGCTTTAGTACCTTACTTAATTTAATTAGTGAAACAATCATTAATCAAAAAGCTGATTAGATGTTAAACCAAATGCTTTATAAAACATTTTTAATGTTTCATCAGCAACTTTTTTAGCTTGGTTTTTACCATCATTTAGGACTTTTAATACCATTTCATCAGTAATTTGTGCTTTTTTAAATTTAAGTGATTCTATGACATTAATAACAGCACTACTGAGGTCATTCTTTAAATCTAAATAACTTTTATTCTGATAATATTTAACAAGATCAGAGCCGATTTTTTTACTTAAATTATGGTTAACTTCTTCTTTTAAAAGTGCAGTTAAAATAACAAGTAAATTAGTAACACCAGGTTGGGTTTTTTTAGCAAAACGAATCTTATTAAAACTATCAGTTGTGGCTTTGCGCACTTTTTTGATGATTGTTTCTTTACTATCATCCAGATAGATAACACCATTTTGATCAGGATTTGATTTGGACATCTTCTTTAAAGGATTTGATAGATCCATGATCCTGTTGGTATCTTTGTTTTCTATAAATACAGGTAATTTCAGTTTTAACTTAAATTTTTTTGCTATACGTTTAGCTAAATCATTGGTTAATTCCAAGTGCTGCTTCTGATCATTACCAACTGGAACAATATCAGGTTGATAAAGCAAGATATCAGCAGCCATTAACACTGGGTAAGTTAACAAACCAGTTGGGATAGTAATGGTGTTATTACTATTTCTTTTTTGCGCTAATTTCTTTGTTTTAAATTGGGTCATTCTTTGTAATTCACCTAGATTACTTTGTGTCAGCATTAGATAACCTAACATGGTATGTTCCATCAGATCACTTTGTAAAAATAAGTTCACTTTTCCATAATCAAGTCCTAGTGCTAATAAAGTTTTAACAAGTTGCAAGTTGTTATCTTTGAGCATTGTTGGTTCAAAATCAACAGTAATAGCATGAAGATCAGCAACAAATAAAAACAGTTGGTATTGACTTTGGAGTTGTTTTAAACCTTGCATTACACCAAGAAAGTTACCTAGGTGTTGTCTTCCAGAAGCTTGAATCCCTGTAATTGCGCGCTTTATCATTATTAGAATTGATAGGGTAATTAAAGGTTATTTTAATGAAAAAAGCGGGTAAAAAAAATAGTGATGCAGGTAAGACTTTTATCTTAATCTCATCATCCTGTTCATCCTGTCAGAAAGCCATTGAGTTTTTTGATCAAAACAAAATTAGCTATGTTGTTGAGAATTTTTATAAAAAACCAATTAGTGATAAGCGTTTTAAAGATATTTTAAGTCTTAGTGAGGATGGTACTGAAAGTTTGTTTTCCAAACGTGCTGATCAGATTAAAGCAACTAACAGTGTTAGTGTTGAAGAGTTGAGTATCAGTGAATTAATTAAGCTAGTTAGAGAACGTCCTTCTTTACTAAGAAGGCCTATTATCATCCAATATAATTCTTCAGGAATTCCTAAAAGGATGCGAATTGGTTATAACTCATCTGAAATTAAGGTCTTTGAGCGTAAACTAATAGAACCAAAGCCTATAATACAACAATAACATCTAAATGAAATACAAAATTTTTGCTTCCACAACGCCCCAAACTGAACCTGTTCTCAATAAGTTAAGAACAGTTTTAAAAACTTGGCAAGCGGTTGAGAATGGTTATGAATATGTGTTTGTTTTAGGTGGGGATGGTTTTTTTGTTTCCACACTTGCTAACTATAACTGTGATAGTTGTAAGGTAGTTGGTATCAATACTGGCCACCTTGGTTTTTACACTTCTTTTAATGGAGATGATCTTGATGAAAATTTCATTTCAAAACTAACTAGCTTTGAGTTTAGAAAGATTAATTTACTGGAAGTGAAAACTGAAAACCACAGTTTCTTAGTTTTAAATGAACTTGCAGTTTACACCAACACTGCATATCCAATTAACATCTTTATTGATGATAACCCCTGGGAATCATACCGCGGTTCAGGGTTGCTAATTGGCCCAAGAACAGGCTCAACTGCCCTAGCAAAATCCGCTAAGGGAGCGGTTATTTTTCCAAATGTTGATGTTGTTCAAATTATTGAACTAAACCCCTTATTACATCCCAACCAAATCACAATTCAATCTCCGATTATTCTGCCAATGCAAACCAAGGTTGAATTTAGGATCAAAAAGGCTTTTAAAGCAGAGCAATTTCCCAATTTTTATGCAGATGGAATCAAGCTTGATTTAAAAAATGAAGATACCAGTATTAGCTTTCAGTTGGTTTTATCAAGATCAATGTTTCACGCTTCTTTAAAAACTAAGGATTTTATTGATAAACTAAAGTCAACTTTTATTAAGCAAAGCTAATGAAATGGTTGTTGTGATTGGGTTATATTTTTAGTTTTGGTTTACTCTATCTTTGGATAGTAAAAAAATCAAAACAAATTGCTCAACAACCCAACACAAAACTGGTTGAATCAACTTCTATTCCTTTTAAAGTTAAGGACTTTGTTAGTGCTTGTGGTGGTAAGAAAAACTTTGTTAATATAAAAACAACACCAACTCAATTAATAGTTACTTTTAAAGATGTTAACTCAGTGAGCTTAACAAAGCTTAATGCGCTTAATATCAAAGGAATTAACAAAAACCAAAACCAATTTCGTTTTGTACTTGGTAACTTTGTAAATGAATTGAAAAAAAAGATAGAAGATGAACAATAACATTACTAACAGTATTGTCCAATTGTTTTTCAACACTTCTCTCTTTGCTTTTCTGTTTTTAATCATTATTGCGTTTAATTTATGCTTGTTTGCCTATCTTTATTTTCAGTACCGAATTTATAAGAAAAACCCTAAAAAAGCTAACAACTTTAAAGCGAATGAATATGAAAAAATTAAGCTATTAAAAAACCAAAATTTCACTGAAAGTAATAAATTAATTGCAACAACTAATGAGTTAAATGAACTTACTAGTCAACTAGATAATATCTTGGTTAGGATTATCAACAAACCACTAGCAAAGTTAGTTAATGATTTTTTAGATGAACAGATTAAACAGATAGTTAAGCTAGATAAAAACAGTTCTGATTTTCACTCAGAAAGTGATAATCTCCCTTTTTATACCAAACTCTTTAATGATTTTCACTTTGGTGTTGATAAATTAATAAACATTAACATAAAAAACCCTCTTTATAACTGGGTTTATAGCCCCAGTTTTTTAATTAGTGAAAGTGATTTTCGCAAGCTTAATGGTATTAGTGGTATCAATAAAAAGCTTTTGGTTGAAAAACTTAGAATTGAAGACATTGTGTTTACAGATCTCAACAAAAAGTATGAAGTTAATGTCTTGACAGAAAGTCCTATTAAAGCACAAAAAACAGTGTTAACTGTGCGTAATATCCTGATGAACGATTATGTTGATAATGAAAGGATTGAATCATATGTCCAACAAGCTAACTTCTTTTTTACTGAGCACTGTAAAAAGATCGGTAAAGAGATCTTAGAATCACTTAATATTTTTATCTCAAGTAGTTCACTACACCGTCATTTTGGCTTTTTAGCATTTCGCTATTCATTTGGACAAAATGTCTTATCCCATAGCCTTGAAACTGCATTTTTAACTGCCCACTTAGCAGCTTTAATAGAACTTGATAGTGAACTGAGTTTAAAGTGTGGATTGCTCCATGATATTGGTAAATCTAATGATGATAATGGTAAAGAGAGCCATACGATTACAGGCGCTAAATTCGCTGAGCAATTTCAACTACCTGATGACATTAAATACACAATTGCTAACCACCACAATAAACATATTGACAATACTTATTGTCGTTTAACACAAATTGCTGATAAACTATCTGCTGCTAGAATTGGTGCTAGAAGTGATAGTTCGCTTCTTTTTAAACAACTAAAAGATGAGTTGAAAAAGATTGTTGATAAAACTATTAATAATTTTCATACAACGATCTTACTAGGTCAAAGTGGTAGAAGGTTAATGATTTGACTTGAAACTAAAAACCAAAATCAACTGTTAAGTAATGAGCAAATTATTGAAATGGTTGAAAAGATTAAAGCTGAAATTGCTAAGAATTCAATTACAAATCACTTCCCTATTAAAGTTGTAATTAGATATAACTTTGAACATAGTTTCAACACCAAAAGCTAAGGATGCAATACAGCGCTTTAATACCACTTTTTATATTACTAATTAGCTTAGTATTATTTTGTTTCAGCTTTAGAAAAAATCAAAGTGAAAATCAGATAGTAAAGATCTTATTTTTTGCTTATTGCATCGATTTTTTAGCTTTAATTCTTGCTGTAATGTTGCTCACTTTTTTAAGTCATGGGTTGTTAAGTTTAGCGATTTTAATCCCTGTCTTAGTTTTTTCAATAATAATGTTTTTTGTTATGGTTATTAGTCATTATCCCCTTATGAAAAGACTATTTGGAAATTAAAATTCTAAGTACCATGGAGTTGAAAACCCCTAATTTTAAGCTAATTGATGAAAAGATTGCTGAATTTAATAAGAGCAATGAAAACCTGATTGTAAAACTACTTCAAAAAGAAAAGGAATTTGCCACAAACCAAGTTACTATTCAGTTTGATACTCAGTCAAAAAAGTCAGAAGAAGTGAAAAAACCTAGTAAAAAAATACTGAAAAATTATCACTTTCTAACAGTAGTTATGGCAGTTAATTTGCTAATAACTGTTGGTTTGATAGTTGGTTTAATTTTTGCTTTCAGATATTAGTTGGTAGTTTTCTTCTAAGGAACGTTTGTTGGTTTTATTGACATTATAGCCAAACCCTTTACCAGTTTCGTATTTGGGTACTATGTGTAAGTGAAAATGAAAAACCACTTGACCTGCAATTGCCCCTTCATTAGAAATATAGTTTAAACCAGATGGTTTTAGTGTCATCTTTAGTTTTAAAGCGATTTGTTTTGCTAATAAACTAACTGCTTGTAACTCCTTTTGATCAGTTGAAGAAAAATTAGCTGCATGTTTTTTAGGGATCACTAAAGTATGACCATCAGCTACAGGAAAAGCATCTAAAAAAGCAATGGCATGCTCATTTTCTCCAATTTTATAGGATGTAATTCAACCTTGAACAATATCACAAAAGATACAACTACTTGTGGTGTTTTTTTCCATAGTAATGGTTATTTATAACAAAAAGCATTCATAAGATTAAAAACATTGATGCAAAAATAGGGATTGATCAAATCCAAACATAACTGTTATATTTAGTTTGCGTAAAAAATCCATACACTGAATAGGAGTTTTCACCATTATCCCAAACATTTAAAAATGGAATAGTTTGGAGATAAAAAGCATAGATACTGGGATAAATGCCTGCTTTTAAAAGAAAGGATTGCAAATTCCTTGGCGGTTTGATAAATTTATAAGTTTTAAAAAGTAATCCAACATAAATAAAGTAAAACAACGGATGGATAACATGGAGTAATAGCTCTGAAAAAGCATTCCAGTCATTAATAAAAGAACCATTAAAACTATATGGTAATTGCGTTGTTATAGTTGAAAACCAACCCTGTACATCAGGTTCTATATTAACAATACCTGTGAAGAGTGATAAAGCATAAAAGTTAAAGAAAATAACTGTAAAAACTAGATAACCTGTAACACTTAAAACCAGTGAACTGTTTTCAAATCATCTGCTTTTATGGTTAAGAAAGTAGTATCATACTAAAAAGATTGCAAGGCTATTACTTTGGTAAGTAAAAGTATCAAAATTAGTAAACCAAATCAGGCTCATTTGACCACTGTTAGCAACACTAATCAGTGCAGTAATCACCATAAAGATTTGGGTGAATAGTGAAAAAGGTGCTAGAAAAATTAAATAATAATCGCATTTATTGTTAAGATAAAAGCAACGGTAAAAAATTCCAATTGATTTTTTAAGCACAATTTTTATTTAATGATATATGAGTTTTAAAAAGATTGCTGAAATGATGCGTCAAGCAGAACGAGAAACTAAGAAAAAAACATTAGCATTTGAACAACAAGCCTTTGAATACAACTATAAAAATGGTGCGATTAAGATCACTATTTTAGGTGATCTTACACTTAAATCAATTAACATCGATCCTGTTTTGATTGATGCAAGCGACAAAGTTATTCTAGAGGAGATGATTATAGAAGCTACTAATGAAGCGGTTAGTGATGTGAAAACTAAGTATGATAACTTAGTTGAGAAAACTATGCCAAAAGTTCCAGGTCTTTTCTAATTAGATTTTCCTTTTTAATAAAGATCTTATCTTTTTTCATTTCCATAAATGTAGGGATATTAGCAAAGAGTGTCATTAGTAAAATCGCAACATAACTTAAACTAAAGCCCACAATAAACACAAGTTGTGGATCACTATTGCTTAAGTTAAGAAAGGTCGCCACATTTTTATATTCACCATTAGTTCAATCACTTGCTAAGATACCAGTTGTATTACCTGTGATCTGGTCCTTAAACACAGACACCATCAAAGCTACATAACTAAAGTAAATTAGAGCAAACACATGGATATAGAAAAATGAGATTAAAGAAAAACAGTTATCCCACAAGATAATTGCTAAGCTTACAAGTAAACTGAGAAAATGCATCATGAAATAAATCTGGTTATTTCCTGTACCAACAAAGATGAAATTGACAATATCTTGTTCATTAACAGGTGTAAAAATTAGTTCACCAAAAAGAGTAACCAAAGCTCCAAATAACCCAAAAACAGCTAACACCCCACTAATCTTTTTTTGCTTTAAAAAGACAAACACTGGCGCAATAACAGCAAAAAATGGACAAAGATCTAATAAAAATATCCGACTTAGTCGTAGTGAATTTAAGGTTTGTGAAGATTGGGAATTTTGTCCATAAACATCAGGGAATAAATAAATAATCTGCCTACCAAAAAAAACATAGACAAACAGGACAATGGCTAGTGCTTGAATTAGATAGAATCTAATCTTTAGTTTTCGAACTAAAGGAGTAAAAGCTAAAACAGAAATTACACCAGCAATAATAAGGATGATGACAAGGTAGTTAAGTGTCTGCATTACTTATTGCTTAAAATTAAGATAAATAATTTTCCAATTTTGTTTTCAATGAGTGATCGTTTAAATGATCAAGCCCAACATCGCTTGCAGAAACTTTTAAGGTTAAAACAAACTAATAATGACCCTTATTTAGTAACAAAAACTAGTCTAACCCATTCTTCAAAAAGCTTTCAAGTTGAATTTGAAAAATGTGCAGAAGAAGAGTTGAAGAAAAAAACAACTGTCTCACTAGCTGGAAGGATCATTGCTATTAGACAAACCTTTTTAATTATTCAAGATTTTGATGGTCAAGTCCAACTTTACATCAATAAAAAAATCCATCCTAAGTTATTTGATTACTTTAATGAATTTGTTGATATTGGTGATCAAATTGTTGTTAGTGGTAAGCCAATGTTAACTAAAACAAAGGTATTAACTTTAGCTGTTGAAGAGATGAAAATCATTGCTAAGTGTTTATTGGTTCCACCTGAAAAGTGACATGGACTTACTGATATTGAAACCCGTGCTCGCAAGCGCTTTCTTGATCTTACCTATAACTTAGCAATGCGTGATGTTTTTCTGAAACGCACTAAGATTATTAAATCAATCCGTAGCTTTCTTGATCAAAATGGTTTTATTGAGGTTGAAACCCCCACTTTACAAGCTGTTTTAGGAGGAGCTAATGCTAAACCCTTTAAAACCCATTACAATGCTTTAAAAGCGGATTTTTATCTCAGAATTGCTAATGAAATAGCATTAAAAAAACTCATTATTGGTGGATTTAATAAGGTTTATGAAATGGGTAAAATGTTCCGCAATGAAGGGGTTGATACTACCCACAATCCTGAGTTTACCAGTATTGAAATATATCAAGCTTATGCAGATTTTGAAGTCATGCTTGTGCTTGTTGAAAAGATGATTCAATCACTTTGTGAAAGCTTAAACCAATTTAGCTTTAACTGAAATAACAAAACGATTAATCTAAAAACACCATTTCATAAGATAACAATGGTTGAACTTATTAAGAAAGTTACAGGGATCGATTTTAATTCAGTAAAAGATGATCAATCTGCCATTTTATTAGCAGAAAAACACCATGTTAAACTAGCAAAACACCAACAAAATAAGCAACACATCATTAATTTGTTTTTTGAACAATTTTGTGAACAAACATTAATTGAACCTACCTTTGTAACCCATTATCCAAAAGCAGTTTCTCCTCTAGCAAAACAAGATCCTTCAAATCCTGAATTCACCCAACGATTTGAACTTTTTATTAATGGTAAAGAGATTGCTAATGCTTACAGCGAGCTAAACGATCCTTTAGAACAAAGAAAAAGGTTTGAACAACAACTTGAAGAAAAACAGCTTGGTAATGATGAGACAAGTGAACTTGATGAATCGTTTTTAGAAGCATTAAGTTTTGGGATGGTAAACACTGCTGGGCTTGGGATAGGTATTGATCGTTTGGTAATGTTGTTATGTGAATGTAATTCTATCCGTGATGTTGTTTTCTTCCCCCAGTTACGTGAACATAAATAGTTTTGATATTTTAATTGTTGGTGCTGGTATTAGTGGAATAGTACTAGCTAACATCTTAGCTAACCACAATAAAAGGGTTTTAATTGTTGAAAAAAGAGATCATATTGGTGGTAACTGTTATGATAAAGTTGATAGTAAAACTCAACTCTTGTTTCACCAGTATGGACCCCATATTTTCCATACTAACAACCAAACTGTTATTAACTTTATCTCACCCTTCTTTGAACTAAATAACTACCACCATCGGGTTGGTTTAAAATTGAAAAATAACCTAGATTTAACCTTACCCTTTGATTTTCAACAGATCTATAAACTAATGGGAAAAGATGGTAGAAAACTCGTTAGTTTTTTTAAAGAAAATTTCAGTTTAAATAGTCATCTATCATTAGCAGAATTACAACTAATTGATAATCCTTTAGCACAAAAACTCTATCAGTTTTTAATTAGTAATGTTTATAAACCATACAGTGTCAAAATGTGGGGTTTACCATTTGCAATGATTAATGAGAATGTTATTAACAGGGTCAAGATAGTTTTAAGTGAACAAAGCAGTTATTTTCCTGATGCAATTATCCAGGGATTACCTAAATCAGGTTATACAAACAGTTTTCTTAAGATGTTAGCCAATCCCTTAATTGATGTGCAGTTAAACTGCAAAGATAACCTTTTAGTTTATCAAGATGAAAAACTGTTTTTTAACAATACTTTAATAGAAAAACCAGTTGTTTACTGTGGTTTAATTGACAAGTTATTTAACTTTTGCTTTGGTCATTTGCAATACCGTTCCCTTGCCTTTAGTTGAAAAAGATTTAACCAAAAAAAATACCAAACTTATCCTGTTGTCAATATGCCTTTAGCTAAATCAATCACAAGGAGTGTGGAATACAAACAACTAACAAACCAAGGTTCTTTCAAACCGCAAACCATCGTTAGTTTTGAAACCCCTGGCAGCTATGCAATTAACGATCCTAGGTTTAATGAACCTTATTACCCAATTAACAATACACTAAATGATACTCTTTTTAAAAAGTACTGAAAAAAAGCAAGTAAGTTAAAGAACCTACACCTTTTGGGAAGATTAGCAACCTACCAATACATTGATATGGATAAAGCAATCCTACTTAGTATTAAAAAAGCCCAACAACTGTTAAGTTAATGGAACAAAAAAACATTAGAAATTTTTCTATTATTGCCCATATTGATCATGGTAAATCTACCTTATCAGACCGCTTGTTAGAACATAGTTTAGGCTTTGAAAAAAGACTATTACAAGCGCAAATGCTTGATACTATGGAGATTGAAAGAGAAAGGGGTATTACCATTAAATTAAATGCTGTTGAGTTGAAAATTAATGTTGATAACAATAACTATCTTTTTCATTTAATTGACACCCCTGGGCATGTTGATTTTACTTATGAAGTGTCTCGTTCTTTAGCAGCTTGTGAGGGAGTTTTATTGTTAGTAGATGCAACCCAAGGAATTCAAGCACAAACGATTTCCAATGCCTATCTTGCGTTGGAAAATAACCTGGAAATTATCCCAGTCATTAACAAGATAGATATGGATAATGCTGATATTGAAACAACAAAAGATTCACTCCATAACCTATTAGGAGTTGAAAAAAACAGTATCTGTTTAGTATCTGCAAAAGCTAACTTAGGGATTGATCAGTTAATTCAAACAATTATAGCTAAGATCCCTCCACCAAAAGGAGAAATTAATAAACCTTTAAAAGCATTACTCTTTGATAGTTACTATGACCCTTACAAGGGGGTTGTTTGTTTTATTAGAGTATTTGATGGTTGTTTGAAGGTTAATGATAAGGTTCGTTTTATTAAAAGTAATTCTGTTTACCAAATTGTGGAACTAGGGGTTAAAACCCCATTTTTTGAAAAAAGAGATCAATTGCAAGCAGGGGATGTTGGTTGGTTTTCAGCAGGGATAAAAAAACTCCGTGATGTTGGGGTTGGTGATACTATTGTGAGTTTTGACGATCAATTTACAAAACCCTTAGCAGGTTATAAAAAGATCTTACCCATGATCTATTGTGGTTTATATCCAGTTGATAACAGTGATTATCAAAACCTCAAGTTAGCGATGAAAAAGATCATAATCAGTGATGCAGCATTGGAATATGAATATGAAACATCCCAAGCGTTAGGGTTTGGGGTTAGGTGTGGTTTTCTAGGTCTTTTACATATGGATGTTATTAAAGAAAGATTGGAAAGAGAATACAACCTAAAACTCATCTCAGCTCCCCCTTCAGTTGTATATAAGGTATTGTTAACCAATGGTAAAGAGATTAGTATTGATAATCCCTCTTTGTTACCAGAACGCTCCAAGATTAAAGCAATCAGTGAACCATTTGTAAAAGTTTTTATTGATTCACCTGATCAATATTTGGGCAGTGTTATTGATTTGTGCCAAAACTTCAGGGGTCAATATGAAAGTTTAAATGAGATTGATATCAACAGAAAAAGGATCTGTTATCTGATGCCTTTAGGGGAAATTATCTACAGTTTTTTTGATAAGTTAAAGTCGATTAGTAAGGGTTATGCATCGTTAAACTATGAGTTTTATAACTACCAACATAGTCAACTGGAAAAAGTTGAGATCATGTTAAACAAACAAAAGATTGATGCATTATCTTTTATCAGTCATAAAGACTTTGCTTTTAAGCGGGCAAAAAAGTTTTGCACTAAGCTCAAAGAATTGATTCCAAAGCATCTGTTTGAGATCCCTATCCAAGCAACAATAGGGAGTAAAGTAATAGCAAGAGAGACAATCAAAGCAGTTAGAAAGGATGTAATAGCTAAACTTTATGGAGGGGATGTTAGTAGAAAAAAGAAGTTATTAGAGAAGCAAAAAGAGGGTAAAAAACGCTTGAAAGCAGTTGGGAGTGTTCAATTACCTCAAGAGCTATTTAGTCATTTGCTGAAAGATGAAGATTAACATTTTTATAATTTGTTATTCTTTTGTATTGGTTGATACTTTAACAAAGTATTCACAATAAAATTTCAACACTAATATAAACAATGATAAAGGATTTTAATCCTGGTGATTTTATTGGTAAAAAACCAACTAAAATCTATGCTTTTGGTGGTATCCAAGAAGTTGGTAAAAACATGTATGGGATTGAATATGATGATGAAATCATCATTATTGACTGTGGCATTAAATTTGCTAGTGATGATCTACTTGGTATCAATGGGATTATCCCTAGTTTTGAACACTTAATTGAAAACCAAAGTAAGGTTAAAGCATTGTTTATTACCCATGGTCATGAAGACCATATTGGGGGTGTACCATACCTTTTAAAGCAGGTTGATATTCCTGTTATCTACGCACCAAGGATCGCAGCATCATTAATCTTGAAAAAGGTTAATGAGCACAAGGATGCTAAGCTCAATAAGATAGTTACTTTTGATGATTTTAGTGAGTTTCAAACCAAACACTTCAAAATTGATTTTTACCGGGTAAACCACTCGATTCCCGATGCTTTTGGAATCTGTGTGCAAACCCCTAATGGCAACATTGTTCAAAGTGGTGACTACCGGTTTGATTTTGCTGCTGGGAGTGAGATGTTAGATGTTCATAAAGTAGTGAAAATTGCTGAGCGTAATGTCCATGTTTTTATGAGTGAATCTACTAATGCTGAAGTACCAGGTTTTTCCCAAAGTGAAAAGTTAATTTACAGAAACATCCAAAAGATCTTAAAAGAAGCAAGAGGTAGGGTTATTTTAACTACCTTTGCATCCAACATCACACGGATTAATGAAATTATTGAGATTGCTTTAAACAACAAGCGCAAGATCTGTTTATTGGGCAAATCAATGGATGTTAATGTCAATATTTCGCGCAAAATTGGATTGATGGCAATTGATAGTAATGATATTGTGGAAGTCCGTGATATCAAAAACTATCCTGATCGTAATATCTTAATCTTGTGTACTGGTTCACAAGGCGAGGAGGCTGCTGCTTTAAACACGATGGCACGTGGTAAGCATAATTGGGTAAGCTTAAAATCAACTGACACCATCATTATGTCTTCAAATCCAATTCCAGGTAATTATGCTGCAGTTGAAAACTTGCTTAATGAACTATCTAAGTTTGGTGTTGCTATTTATGAAAATTCATCCCAACTAAAACTACATGCCTCAGGTCATGCCACCCAACAAGAGTTACAGTTGATGCTAAATTTAATGTTTCCTAAATACTTAATTCCTATCCATGGTGAATTTAAGATGATGCGAACCATAAAAAACATTGCTAATGAATGTGGTATTAAAAGCGAGGATGTGGCACTTTTAAGTAATGGCCAAGTAATGTATTTAATTGATGAAGAACTTTATTATTCCAATGAAATTATTAATGCTGATCCTATTTATATAGAGAGTCATAACTCTTCTCCTGATCTTGCAAGAATAATTAAGCAAAGACAAATCCTTAGTCGTGATGGGATGTTTGCTGTTATTGTTGTTTTTGATAAAAATAACAACATCATTGGGATTCCAACCTTAATAACAAGAGGTTGTTTCTTTGCACTTGATTCCAATCCTTTAATGACAAAGATAGCCCATTCTGTTAAAAGAACTTTAGAAAGTGTTATCCAAAGTAAGAAGTTTAATAGTCATGAACAACTAACAAAGGAATTGAAACGAGTTTGTAAGGAAACTGTTTCTTACTTTATCTGAAAAAATAAAAACCGTAATCCCTTAATTTCAACTGTGCTTTCCTGGATCTAATCCCCCAACCTCTATTTTGTGTTACTAGTGCCAAAAGTGGTATTGGAGTATCACAACCTGAATAACCAAGTAGTCAAAGAGAGTTTGGAAGTGGAAGCAACTGATTCTTTCAACCCCACCCAAAGGTTGCAAAAAGAGAGTCCAGAAAAGGATGATAGTAAAAGTCAGGAGAAACTCCAAGAGACCATGTCATCAGTGAGTATGAGTGGGGCTACCTCTACTCGAACCAAGGCCCTCAAAGTGGAGGTGGAAAGGGGTAGTAAAGTCAACCAAGGCGAACTACAATCCAACGACTTTGCCAAAAAGCCGTTTAAAGATGAGAGCAATAAGAAGTTGGATGCGAGTGGGGAGTTTGCCAATGATAAAGCCTGAAAACCGGTGTTGACAACAGATGAGATAACAAGAAATAGGGGGATGGGGGCGACTTAGACTTTCTCCCCTGAATCGGCAATGGTAAACCCTTCTCCAACTCCCCCTCCCCTTCAACTTCTGCTTCCTCAACCCCACTCCCCACTTTTTCTAACATCAATGTTGGGGTTAAATCAATGATCACTCAACATTTAAATAAAGAAAACACCCGGTGGGTGTTTACCCCTAACTCTTCACCAGACATCTGAACGGGAGCAGGTTATAGAAAAGCTAATAACAACAATAATGGCATTCCTTTTGAAAAGGTGAAACCTAGCAATGGTAGTACCACGTTTAATCCCTCCTCGGTAGCTCCCCAGCCAAAAAAACAACCACCTATTCCTTTTTACCCAATTCCATCAGTCCCACCAGTGACTGGATCAATGCATTGACTTTCACTAATAAGAATAACCCGCAACGAAATCAGTTGTTGCTCAGAAGCTTACTAGGAACGATCCCGGTATTGATCAATAAGAGTGGGGATAGTAATGATCAATTTAACAAGGATAGTGACCAGAAATGGGATAAAACGAATGAAAAAGATGGGAATTTACCTGGGTTTGGGGAGGTGAATGGTGGTTTTTACCCAGTTTTTACTTATTTAATAGTTAAAAAAGTTTTAGATTTTTCTTAGTTTTTTATCTATTTAATATTTAAGAAAGTCTCAAATTTTTATCAGTTTATTGGTCAAAGAAGTCGCAAATTTTTCTTATTTTTTTATTTTTTTAATATTAAGAAAACGTTAGGTTTTTACTTCGCCACCCCTATCACCGATTCTAAAAGTGATCTGGTTAGTTTGGCACAACTTGATTCTTCCTATCAAATCTCCGACCAAACCATCCATAACACCAACTTGTTTGTGTTGTTCAAGTCTACTCAGGTGAAAGTTAAGTATGAGTTCAAGTGGCTCAAATACCAACACAATTAGTTTTGATTCAACTAGTCAAGCTAACAAACCATCCTACATCGTTGAGTTTACTAACTCCACCAACATTGGCATCAAGTGAAGGATGGTGAAAAAATATCAGTTAGATCTACCAAGTGTTTCAACAACCATGAACCAAGTGTTGCAAGAATTGATCCTAGAACAACCTTTGACTAAGTATACCTTAAATTCGAGTTTGGCCAAACAAAAGGGCAAAAGCCAGATAGAGGTGCATCTGGGTAGTGGGCAAGCAAATCAGTGAAACAGTCATGCGCAACCACCACAATCTAAACAACAATCCCTCTCCCAATGCATCAACTGGATTTAAATTAACAAAGGCAATGCGTATAGAAAACTAAGTGAATCCTGACCAATTTACCAACCAATTGATGGGACCAAGCAGGGCAAAGGGAAGGATAGTAGTGGGTGGAATAGTGAAGAAAATACGGCAGCAGGGGATGCGCCCAGTGTTACTGGAGGGGGAACATCTTCTGGAACATTTAATAAATACCTCAACACCAAACAAGCGTTAGAAAGCATCGGTATCTTGTTTGATGGGGATGGAATGAGGAATGTGGTTACCCAGCTCTATTATGCTTCAACTAGTAAGCTAGCAGTCACTAACAACCACATTGTCGTGATGGGTAACAGCTTTCTACCCAGCATGTGGTACTGGGTGGTGGATAGGAGTGTAGTAGTAGTAGTAGTAGTAGTACCAAAGACCAAGCACTTGCTTGATCAACAACAACTAGCTTAGATAGTAAAACGGGGTATAGGGATCTAGTGACCAACGACACGGGATTAAATGGTCCAATCAATGGAAGTTTTTCAATCCAAGACACCTTCAGCTTTGTTGTTCCTTATTCGGGGAATCATAGTAATCAAACTTCATCAGGACCCATTAAAACTGCTTATCCGGTGAAAAACACAGAAAAATCAACTGTCAAGATCAATTCCTTGATCAATGCTACGCCGTTGAATAGTTATGGGGATTTAAACATTAAATAAAGCGAATTCACCCAAATTATTTACTTATTTATTAACTATTGTTACCCAATTTTTCTCTTTTTATTTGTTGTTTTTTTACTAATTAAATAAGCAGTCTTTCTTACAAAAAAGAAAAATTCATATATAATCTTTGCGCTGTTAACACCTTTGTTAACGCCAAAAATGTTCTTTCAAAACTGGATGCAATCTGTCAATTTTTTCTGAGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCATGCCTAATACATGCAAGTCGATCGGAAGTAGCAATACTTTAGAGGCGAACGGGTGAGTAACACGTATCCAATCTACCTTATAATGGGGGATAACTAGTTGAAAAACTAGCTAATACCGCATAAGAACTTTAGTTCGCATGAATTAAAGTTGAAAGGACCTGCAAGGGTTCGTTATTTGATGAGGGTGCGCCATATCAGCTAGTTGGTAGGGTAATGGCCTACCAAGGCAATGACGTGTAGCTATGCTGAGAAGTAGAATAGCCACAATGGGACTGAGACACGGCCCATACTCCTACGGGAGGCAGCAGTAGGGAATTTTTCACAATGAGCGAAAGCTTGATGGAGCAATGCCGCGTGAACGATGAAGGTCTTTTTGATTGTAAAGTTCTTTTATTTGGGAAGAATGACTCTAGCAGGCAATGGCTGGAGTTTGACTGTACCACTTTGAATAAGTGACGACTAACTATGTGCCAGCAGTCGCGGTAATACATAGGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCAAGCGCAGGCGGATTGAAAAGTCTGGTGTTAAAGGCAGCTGCTTAACAGTTGTATGCATTGGAAACTATCAGTCTAGAGTGTGGTAGGGAGTTTTGGAATTTCATGTGGAGCGGTGAAATGCGTAGATATATGAAGGAACACCAGTGGCGAAGGCGAAAACTTAGGCCATTACTGACGCTTAGGCTTGAAAGTGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACACCGTAAACGATAGATACTAGCTGTCGGAGCGATCCCTTCGGTAGTGAAGTTAACACATTAAGTATCTCGCCTGGGTAGTACATTCGCAAGAATGAAACTCAAACGGAATTGACGGGGACCCGCACAAGTGGTGGAGCATGTTGCTTAATTCGACGGTACACGAAAAACCTTACCTAGACTTGACATCCTTGGCAAAGTTATGGAAACATAATGGAGGTTAACCGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCGTTAGTTACATTGTTTAACGAGACTGCTAATGTAAATTGGAGGAAGGAAGGGATGACGTCAAATCATCATGCCCCTTATGTCTAGGGCTGCAAACGTGCTACAATGGCCAATACAAACAGTAGCCAACTTGTAAAAGTGAGCAAATCTGAAAAGTTGGTCTCAGTTCGGATTGAGGGCTGCAATTCGTCCTCATGAAGCTGGAATCACTAGTAATCGCGAATCAGCTATGTCGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACTATGAAAGCTGGTAATATTTAAAAACGTGTTGCTAACCTTTATTGGAAGCGCATGTCAAGGATGGCACCGGTGATTGGAGTTAAGTCGTAACAAGGTACCCCTACGAGAACGTGGGGGTGGATCACCTCCTTTCAAATGGAGTTTTTATTTTTTATTTATCTTAAACACCCATTAATTTTTTCGGTGTTAAAACCCAAATCAATGTTTGGTCTCACAACTAACACATTTGGTCAGTTTGTATCCAGTTCTGAAAGAATGTTTTTGAACAGTTCTTTCAAAACTGAAAACGACAATCTTTCTAGTTCCAAAAATAAATACCAAAGGATCAATACAATAAGTTACTAAGGGCTTATGGTGGATGCCTTGGCACTAAAAGGCGATGAAGGACGTGTTAACCTGCGATAAGCTTCGGGGAGGTGGTAAAAACCTGAGATCCGGAGGTGTCCGAATGGAGCAATCTGGTAGCTTGCAAAAGTTACCATTAATTAATGAATTCATAGTTAATTAAAGCGATACGTGGTGAAGTGAAACATCTCAGTAACCACAGGAAAAGAAAACGAATGTGATTCCGTGTGTAGTGGCGAGCGAAAGCGGAACAGGCCAAACCTATCTGAGGATAGGGGTTGTAGGGCTTGCATTATGGAAGTTAAAAGGATAGAAGAAGCTGTTGGAAAGCAGCGCCAAAGAGGGTGATAGCCCCGTATTTGAAATCTTTTTAATACCTAGCAAGAAACCTGAGTAGCTCGAAAAACGTTATTTTGAGTGAATCTGCCCAGACCATTGGGTAAGCCTAAATACTAATTAGTGACCGATAGCGAAACAGTACCGTGAGGGAAAGGTGAAAAGAACCCAGAGATGGGAGTGAAATAGATTCTGAAACCATATGCCTACAACGTGTCAGAGCACATTAATGTGTGATGGCGTGCGTTTTGAAGTATGAGCCGGCGAGTTATGATAGCAAGCGTTAGTTAACCAGGAGATGGGGAGCTGTAGCGAAAGCGAGTTTTAAGAGAGCGTTTGTTTGTTATCATAGACCCGAAACGGGTTGAGCTAGTCATGAGCAGGTTGAAGGTTGAGTAACATTAACTGGAGGACCGAACCGACTCTCGTTGAAACGATAGCGGATGACTTGTGATTAGGGGTGAAATTCCAATCGAAATCCGTGATAGCTGGTTCTCGTCGAAATAGCTTTAAGGCTAGCGTAAGATCACAAATAAGTGGAGGTAAAGCTACTGAATGTATGATGGCGCCACCTAGGCGTACTGAATACAATTAAACTCTGAATGCCATTTATTTTATTCTTGCAGTCAGACAGTGGGGGATAAGCTTCATTGTCAAGAGGGGAAGAGCCCAGATCATTAAATAAGGTCCCCAAAATATACTAAGTGGAAAAGGATGTGAAAGTGCTAAAACAGCAAGGATGTTGGCTTAGAAGCAGCCATCGTTTAAAGAGTGCGTAACAGCTCACTTGTCGAGTGTTTTTGCGCCGAAGATGTAACGGGGCTAAGTATATTACCGAATTTATGGATAAGATATTTTTATCTTGTGGTAGACGAGCGTTGTATTGGAGTTGAAGTCAAAGCGTGAGCATTGGTGGATCCAATACAAGTGAGAATGCCGGCGTGAGTAACGCTTGGGAGTGAGAATCTCCCAAACCGATTGACTAAGGTTTCCTGGACCAGGGTCGTCCTTCCAGGGTTAGTCTGGACCTAAGCTGAGGCTGAAGAGCGTAGGCGATGGACAACAGGTTAATATTCCTGTACTTACAGTTAGACTGATGGAGTGACAAAGAAGGTTATCCACCCCCATTATTGGATTTGGGGATAAACCATAAGGTGGTACAATAGGCAAATCCGTTGTGCATAACACTGAGTGGTGATGTCGAGTGAACGAGTGATCAAGTAGCGAAGGTGGCAATTAATCATGCTTTCAAGAAAAGCTGCTAGGGCTAATTTAACTGTAACCAGTACCGAGAACGAACACACGTAGTCAAGGAGAGGATCCTAAGGTTAGCGAGTGAACTATAGCCAAGGAACTCTGCAAATTAACCCCGTAAGTTAGCGAGAAGGGGTGCTTATCTAAAAGTAAGCCGCAGTGAAGAACGAGGGGGGACTGTTTAACTAAAACACAACTCTATGCCAAACCGTAAGGTGATGTATATGGGGTGACACCTGCCCAGTGCTGGAAGGTTAAAGAAGGAGGTTAGCAATTTATTGCAAAGCTTTTAACTGAAGCCCCAGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTAGTCGGGTAAATTCCGTCCCGCTTGAATGGTGTAACCATCTCTTGACTGTCTCGGCTATAGACTCGGTGAAATCCAGGTACGGGTGAAGACACCCGTTAGGCGCAACGGGACGGAAAGACCCCGTGAAGCTTTACTGTAGCTTAATATTGATCAAAACACCACCATGTAGAGAATAGGTAGGAGCAATTGATGCAAGTTCGCAAGGATTTGTTGATGTGAAATGTGGAATACTACCCTTGGTTATGTTTTGTTCTAATTGGTAACTGTGATCCAGTTTCAAGACAGTGTTAGGTGGGCAGTTTGACTGGGGCGGTCGCCTCCTAAAAGGTAACGGAGGCGCACAAAGGTACCTTCAGTACGGTTGGAAATCGTATTTAGAGTGTAATGGTATAAGGGTGCTTGACTGTGAGACTTACAGGTCGAACAGGTGAGAAATCAGGTCATAGTGATCCGGTGGTTCAGTATGGAATGGCCATCGCTCAACGGATAAAAGCTACTCCGGGGATAACAGGCTGATACTGCCCAAGAGTTCATATCGACGGCAGTGTTTGGCACCTCGATGTCGACTCATCTCATCCTCGAGCTGAAGCAGGTTCGAAGGGTTCGGCTGTTCGCCGATTAAAGAGATACGTGAGTTGGGTTCAAACCGTCGTGAGACAGGTTGGTCCCTATCTATTGTGCCCACAGGAAGATTGAAGAGCTTTGCTTCTAGTACGAGAGGACCGGAGCGAGGACACCGCTTATGCTCCAGTTGTAGCGCCAGCTGCACCGCTGGGTAGTAACGTGTCTATTAGATAAACGCTGAAAGCATCTAAGTGTGAAACTATCTCAAAGATTAATCTTCCCATTTCTGTTAAAGGAAAGTAAGAGCCGTTATAGACCATGACGTTGATAGGTTACAGGTGTAAGCATAGTGATATGTTGAGCTGAGTAATACTAATTGCTCGAGGACTTATTGGTTGATAAAAGGTATTTATCAACTAAAAGATTGTCGTTTTTGTTTGGTGCTAATATCGCTGTGGAAACACCTGGAACCATCCCGAACCCAGCAGTTAAGCACAGTGGAGCTAAATGTAGGTAGTAATACTGAGAATAGGTAAGCACCAAGCAAAAATTAAGGACTATAGGTTTAAAAACCTATGGTCTTTTTTTATTTAATGAATAGTTTTTATAACTAATTTATCTAAAAAAATCGAACAACCTTTGACTAAGTATACGCTTAATAGTAGTTTGGCCAAACAAAAGGGCAAAAGCCAGATAGAGGTGCATCTGGGTAGTGGGCAAGCAAATCAGTGAAACACCATGCGTAACTCCATCAGTCTAAACAACAATCCCAGCCCCAATGCTTCAACCGGATTTAAATTAGATAAAGGCAATGCGTATAGAAAACTAAGTGAATCCTGACCAATTTACCAACCAATTGATGGGACCAAGCAGGGCAAAGGGAAGGATAGTAGTGGGTGGAATAGTGAAGAAAATACGGCAGCAGGGGATGCGCCCCTTGTTTCAACAAGTGGAGGTGGTTCTTCAAATAAATTCACCAAGTACCTCAACACCAAGCAAGCATTGGAAAGGATCGGCATTTTGTTTGATGGGGATGGAATGAGGAATGTGGTCAGCCGCTCTTCCAACTCTACCAACCCAACAAGGTGAAGGCTTACCAAACCACTAACACCTACAACAAGTTGATTGAGCCTGACAAGTGAGAATCATCAAGTGATTTGAACAATATGACCAGCTTGTTAAAACCCCTGACAACTAAAAACATCAAACAAAATTGGGGAAGGACACCCAATCAATGGGAAAATAATGGAGGGGGTGTTAGTCAAACCTTTTCGTTTGTTGTTCCTTATTCGGGGAATCATAGTAATCAAACTTCATCAGGAACCATTAAAACAGCATATCCGGTGAAGAAAGGTGAAGCTTCCCAAGTAATGATCAATTCTTTGATCAACGCTACGCCGTTGAATAGTTAAAAACCTGATTTATTTTTATAGAAACAGTTACTTTGAAGATTCACTACTAATAATTTAAATTAAGCACTCATACTAATATATTAATCCCTTTAGATTGCTTAACTCTTTAAAACTTCCCTTTATAATCAAATATCGCCATGAACGACTGACAGTGACTGAAAAACAGACTTGTTAATTCCAAAACCAAATCTGTTAGTTTCTGATTACCACAAACCTCAAGTAATATCATTGATATTGCTGAATTAATTAAGTGCTGTAGTGAGTTAAAAAACACTTCTATTAATGGTTTAATTGACTTTTTGAACCAACAGGATAAACTTGAATTTAACTTAACAAGATTAAAGGAGATAGATGTTGAAGATGGTAAGCAACTATTTGGGATAGAAACTAGTGTTTATAAACACTTTCAAAATGAAATTGCTCGTTTTTATAAACAAGTAAACAAACACTTTCGTGAAACAGGTAGTGAAAGTTTGTTTTTAGCTTTACCAGTTATTGAAGGGATTAATGAGTTTAACGATATCTTTCGAGCTCCATTACTTTATGTTGGAGTTAAACTCAAAGTTTCCCCACGCTTTGAACGTTTCTGATTAGAAATTAACAAAGAAGAGATCTTTTTAAACCCTACTATTATTGGAGTTGAAACCAACAAACGTAATAGTTTGTTTAAAAATAACTATGATACTACTAAGATAGATGTTAATGATGCTTTAAAGGTATTTAGTGAACTTGAATATGAGTTTAGAATGCCTTTAACTTCTGAATTGAAGAGTTTTAGTAAAAAAGCAAAGAGTGATTTTAATACTGAAAAACGAACTAACTATCTTATTAACAACGTTCTTTTGGGGATCTTTGATGTTAAGGGTGATCAGCTGTTCCAAAACTTTAATGAGATTCTAAACACTGATCCTGATGTATTAGATGAACTTCTAAAAGATAGAAGAGATCTGTTGTTAGAAAACCGGGAATTCCGTGAACAATTTGATTTAAAAGATACCTATCTCTTCAGTCACCTTGATATCTACCAACAGTATGCAGTTAAGCAAGCTTTACTTGGTGATTTAATTATTGAAGGCCCACCTGGCACAGGGAAATCTGAAACAATTGTTAATATCTTAGTTAACCTTGTTTTAAACAACAAAAAAGTATTGTTTGTTTCTGAAAAAGTAACTGCACTTGATGTTGTTTACAACCGTCTTGGTAGTTTTAAACACATCGCACTTTTCAACGCTAGTGTTGCTGCTGAAAAGAAACGCTTTTATAACCAGTTTGCTGAGTTTGAAACTTATTTCACTACTTACTTTTCCAAGAAAGATTTGGATGCTACTTTACCAACATTTGAAGGTAAATGGGTGGATGATATTTTAGAGGCATTTCAAGCATTACAAGCTCTTTATGACACCAAGATAAATTCTGGTGAAAATCTGTTTAGTTTCAAAGAGATTGTCAGTAGCTTTCAGATGTTGGATGCTAGTTACATCAAGATTAAAGAATATGAACGTTTTGATGAGTGAGTGCGCGTCTTTTCAAATCCATTGTGATTAGAAAAACACTTAAGTTACCAAGAGTTGAAAAAAGAACTTAGTCAGCGCTGGAATGGTATTGATAATTTCTATCAGTTGCAATCGCTTCTAAACCAAACCAAAAAACGAAAGGTCTTAAACTATGTGTTGGAACACTTTGAACAGTTTAATACAGTTATCAGTCCTAAGCATGTTTTGTTCTACAAGCCTAGCAATAAATCACAATTGCTCTTAAAACAACTGAAACAGGATGTTGAACAATACACTAGTTTACAACGTTTCCAATCTCCTACTAAGTTTGAAACAATCAAGTTGAATTTCATCAACCAAGTTAATGAAAACCCAACCCCATGGTTCTTTTCTTGATTTATCCAATTTCATGCCAAGCCACTGTTGGAAAAACTCGTTAGTTTTGAGTCAAACATTATTAAAACAAAACAAGCTTATCTTAATGGGATTGAAAGCTATGTAGCAAGTTGTAAGAAACTGCTTAAAACAACTATTTTAAACAACTTTTTTCAGCTTTATCAAACTAATAAAGATGAACTATTGGAGATCTGCAGACAAGCAAAAAACCCAGTTTTAAAAGAGATTACTTGGTGGTTTAAAAAACATTTTGAACTCTTAAAAAAACTCTTTCCAGTGCACATTATGACCCTTGAGTCTGCAGCAACTCTAACCCCTAACCAACGTGGTTTGTATGACTATGTGGTTATTGATGAAGCTAGTCAAGTATTTCTAGAAAGAGCAATTCCTATCTTATTTAGAGCTGATAAGTACATTATTGCAGGGGACACTAAACAGTTAAAACCAGCTAACTTCTTCCAATCACGTGCTGAGTATGATGTTGATGAGGAGTTTGAAGATGGCAATATAGAAGCTGCTGTTCACTCCAGTTCTCTACTTCATTTTTTAAAGAACCGTTCAAGAATCTTAACTTTACTTAAGTTCCACTACCGCAGTGATTCAGCTGATCTGATTGCTTTTACTAATAACAGGATCTATGACAATGAATTAATCTTTATGAATAAAGCTAATGCTGATCAAAGGGTTTTTATTGTCCATGATGTAATAGATGGTATCTGAAAAAACAACCGGAATCTCCAAGAAGCACGTGATGTTGTGCAACGCTTAGAACAACTAACAACAACCAATGATTACAAAAAGTCACTTGGTGTAATTTGTTTCAATAAAAACCAAGCAGATCTAATTGAATACTTAATTGATAAACAAAACAATCCGCTACTTAACGAGTGAAGAGAAAGACAAAATGATGTTGGTGAATATGAAGGATTATTTGTGAAAAACATTGAAAATGTGCAGGGTGATGAAAGGGATATCATCATCTTCTCTTTAGGTTATGATCGTTCAGTAAATAGTTATGGTCCTATTAGTAAGCAAGGTGGGGAAAACAGACTAAATGTAGCGATAACTAGAGCTAAACAAAGAATAGAACTGTTTAAGACAAATAGAGGGGAAGACTACAATGGTTTAAACTCCAGTTCTTTGGGTAGTAAACTGTTAGTTGAATATCTACTTTATTGTGAAGCAATGGCTAAAAATCAGGGTGAGAAAATCACTTTTCAAGCGGTAAAAAGAAAAGAAACAAAAGCAAAGTATGAACTAGCAGTTGAAAATGATTTCTTCAACCAACTGCAGGCAATTTTTGGCGGAGAGTTTGAGATTAAACGTAACGTTAATGAAGGAGCTTACTTTTTCTCATTTGTCTTTTACTTTAATAATATCCCTTATCTTGCCATTGACTTTAATATCCCCATTCCCACTTCAAGAAAACAAGTTATGGAAGGGATTTTATACCGTGAACAGTTTCTCAAAAAACGTCAATGGAACCTAATTAACATCTGGATTGATGAGTGAAAATTAAACCCAATTGGGGTGATTTCTAAAATCAGATCAAGTTTAGCAGTGCATCAAAACCAGCATGAAGAAATATAATTAGATGAAGATAACTTTCATTTCTGGACAAGAAGTGTCGTTAGGCACTTCTTTTTTATTGTTTTCAAAAAATAGTTATGAATGAATTAAACCAACCCTTACTTGCTATTATTAAAAATGTCGCTAAAACCAAAAACCTTTCTATAGAAGAGGTGGTTTTTTGTTTGAAAACAGCTTTAGAACAAGCCTATAAAAAACACCTTAACTTTGTTAATGTTGAAGTTAACATTAACTTTGATAAGGGGATTATTAATGTTGAACAACTCTTTAATGTTGTTAGTGATGACAATGAAGATTATGATGACTTTCTTGAAATCCCTTTACAAGCAGCTAACAAAATAAACAGTTCATTGCAATTAGGTGATGTGTTGCGTAAACCAATCCCCTTAAAAAACATTAGTAGTGATCTTATCAATAAGATGATTGCTATCTTTAACCAAAAGATTAGTGAAACAAACTTTAAAGCAGTAATGAGTGAGTTTAGTAGTGAGGTTGGGGAAGTGATTGAAGCGAAAGTTGAAGATATTGATACTAACAAAGAAGGTGGTTTAAAGGGTTATATTATTAACCTTGAAACTACAAAGGGTTATATCTCCAAGCGGGAATTGTCAAAAAGGGAGCGCTTAGAGATAGGTAAAAAATACCTCTTTGTTATCAAAGAAATCCAACGGCAAGCATCGTTATGACCAATTACTTTATCAAGAAGTGATACCCGCTTACTACAGTTTTTGTTAACTTCAAATACTCCAGAAATTGAAAATGGTACGATTGTAATCAAAAAGATTGAACGTTCCCCAGGAGTGAAATCAAAGATAGCAGTTATCTCCAATGATCCTGCAGTGGACCCAGTTGCTGCTATCTTAGGACCTAAGGGTGAGAAGATTAGGGGGATTAGTGAGGAATTTAATGGTGAGATTATTGACATTGTCTTTTGGAATGAAGACAAGTTAAAGTTCTTAATTAATGCCATTTTACCTGCAGAAGTCATTGGTTATAACATCTTGCAGGATGATGAGCGTGATACTAGTATTGAAGTTGTTGTACCTGCAAACCAAATTGCTAATGTTTTTGGTTTTAAAGGTGTAAACATTAGGTTAATTAGTAATTTAACAGGTTGAAATAGTGTTGATGTTTACAGTGAAAAAGATGCAAGTGAAGCCAACATTAAATTCACGAGGTTAAGCTTTGAACCTGAAGGGTTGTTTGGCATCAAAAAAAGAAGGGAAAAGATCATTAGTAATGATGCTACTGATAAAGTCTTTTACACCTCTAAAGACAATGTGATAGATGATGAGATTATTGTTGATTTAGCTAAAGATCTAATGGTTGATAATAAACAAAAACAACCTGAGCAAGTTGCAAAGCAAGTTGTTGAAAAATCACAATTAGAAAAACAAGTTACTCCAAAAGAAAAAGAGAAAGTTCAACCAAAAGCTAAGGTTCATTCTAATAGCCATTCCAAAAAACCAGCTAAACCTAATCAGATTTTTTCTATCACTGTTGATGCTAGTGATAAGAATCTTAAAAAAGATCAAGTTGATAATAACCAAACAAACCCCCAAACAAAACAAACATTTGATAGCTTTGATGATCTTTAATGCAACTAATAACAAGACTTTGTTTATTAACAAGAAAACATTTTGTTAAAAGAGAACTTTTACGTCTTGTAAAATTAGACAACCAACTTGAAATTGATCTTAATCAAAATCTCAAGGGCAGGGGTTATTATTTGAGTGTTTTTGGTTTAAAGCTAGATAAAAAACACCTCAAAGCTGTAGTTGAAAAACACCTTAAGGTTAGTTGTAATGATGCAAAGCTTACTGCAATGATTACCGCCTTACAACAATTAGCACAAGATGAAAAAAAATAGAGCTTTCAACCAGGTTAAAAAACAAAGTTTGACGGTAGGATTAAAACCAGTGCCAAACACCAGTTACGTAATGTTAAAACCGGGGTTAAAGATGGTGTTTTTATCTATAAAGGTCCTTTAACTGTTAGTGAGTTTGCAAGTAAAACTAATATCGCTGTTGCTAACATTATCAAACACTTTTTTTTAAATGGTTTGGCACTAACAGTTAATTCAGTTTTAACAAATGAACAGTTAGCAGATGCATGTGTTAACTTTGGGTTTGACTTTAAGATGGAAACTGAAGTTACCCATGAAAATATTGTAGCTAACATCCAGTTTGAAGATAGTGATGATTTATTGCAACCAAGACCACCTATTGTTACTATCATGGGTCATGTTGACCATGGTAAAACTTCGCTTTTAGACACAATTAGAAAAACTAATGTAACTGCTAAGGAGTTTGGCGGAATTACCCAAAAAATTGGTGCTTATCAAGTGAAAAATCACCAAAATAAAACGATTACTTTTATTGATACTCCTGGGCATGAAGCATTTACTTTAATGCGTGCAAGGGGTGCAAAAGTAACTGATATTGTGGTGTTGGTTGTGGCAGCGGATGATGGGATTAAAAAGCAAACAGAGGAAGCAATTAGCCATGCTAAGAGTGCTAACACTCCTATCATTGTTTTTATTAACAAGATGGATAAACCAACTGCTAACCCTGATCTGGTGATCCAACAACTCAATAAGTTTGATTTAGTTCCTGAGGCTTGGGGTGGGAAAACTATCTTTGTAATGGGTAGTGCTTTAACTGGTCAAGGGATTAATGAGTTGCTTGATAATATCTTGTTGCTAGGGGAAGTGGAGGGTTATCAAGCTAACTATAATGCCCATTCATCTGGTTATGCAATTGAAGTACAAACTTCAAAGGGACTTGGCCCTATTGCCAATGTCATTGTAAAAAGGGGTACTTTAAAGTTAGGTGACATTGTGGTGTTAGGGCCTGCATATGGAAGAGTTAGAACGATGCATGATGAAAATGGTAATAGCTTAAAACAAGCAACCCCTTCAAAACCTGTGCAGATCTCAGGGTTTGACATTATGCCTGTTGCTGGGGAAAAGTTCATTGTTTTTGATGATGAGAAGGATGCAAAGTTAATTGCTAACAAGTTTAAAGAACAACAAAAACAAAAAGCTAACAACTTAACAGTTAACCAAACCTTGAAAGAACAGATTAAAAACAAGGAAATTAAGGTATTAAATTTGATCTTTAAAGCAGATAGTGATGGTTCATTACAAGCTATTAAACAAGCAGTTGAAAACATTAATGTTGCTAAGATCTCACTTAGTATCATCCATGCTGCAGTGGGGCAGATATCAGAGAGTGATATTATGCTAGCAAAAGCATCAGGGGCTTTATTGTTTAGTTTAAACTTAGGTTTGAGTCAAACTGTAAAAAACATTGCTAGTTTACAAGGGGTAAAATTAGAAGTTCACTACCATATCCCTAAACTAGCAGAGGAGATTGAAAACATCTTAAAAGGTCAATTAGATCCTGTTTATGAAGAGATTGAAATAGGTAAAGCGGAAGTTTTACAACTCTGATTTCACTCTAAAATAGGTAATATTGCAGGAACCATTGTTAAATCAGGAAAGATAAAAAGAGGGAGTTTATGTAAGTTATTCAGAGATAAAGAGATTATCTTTGAAGGTAGAATTGACTCTTTAAAAAATGAAAAAACGCCTGTTAATTTAATAGAAACAGGGAAGAATTGTGGGATAGTTATTAATGGTTGCAATGATATTAAGATTGGTGATATCATTGTTGCTTATGAAAAGCAGATAGTTAAAGATGGCAAGCTATAGAAAACAACGGATTGAAAATGATATCATCCGCTTAATTAATCGCACGATTATTAATGAGATCTATGATCCTGTTGTTAAGTTAGGTCATGTTAGCCATGTGAAGTTATCAGCTGATTTTTTTCATGCAGTAGTTTATCTTGATTGTTATGATCGTAGTCAGATTCAAACTGTAGTTAATGCTTTTAAAAAGGCTCAGGGCGTTTTTAGTCAAATGTTAGCACAAAATTTGTACCTAGCTAAAAGTGTAAAACTCCACTTTGTGAAGGATGATGCAATTGACAATGCTTTGAAAATAGAACAGATAATTAACTCTTTGAAAAACTAACAGCTTGAAGTAAAATTAATCCCAATGGACCCACAAAACAAGTCCCCAAAACCACCAGTTAAATCAACTAGATTGGTTGTCAAAAAACAACCAGCAGGTGTTGTTTTTCCCAAATTGAGTATTCCTGTTAATGATTTTGAAAAAACAGTTACTTTAACAAGAGCACAAAAAAAGGAAGCTAAATTTCTCAAAAAAGCCCAAAGGAAAGCTAATAAGCTTAATAATAAACAAGATAGTATCTTTTTCAATTCTGCTTCAGTAGGTGACACTAATAACACCATTCTTCCTCCTGGTGTTAAAAATCAAGCTGATAATAAAACTAACCGTTTTAGTAAATTTATTAGTTTTTTCACTTCTTCAAAAAACAAACAACCAGATGAAATAACAGAAAGGTTAGTTGATGATCCTACTGTTAAAAACCGTTTTAGTGCTTTTAATAAGAAGCTTATTTGAGTTCTAAAGGATAAAAAACTAAGAGCAAGAGCGTGGAAGATTGTTGGTTATACCAATTTAGTTATTGTGGCATTTTTTGCTGGACTTTTAGCAGTGATGAATAAGTTCATCACCCTTTCCTCAGTTGAATATCCTGCTATTGCTTTACAACTCCCTATTAACAATGCATTATGAGGGATTTCTATCTTTGTTATTAGCATTGTTACTTTACCGTTTTGAACAATGTTTATCTTGTTTTTAATGGGAGTAAAGGATGTGAGAACTTCGCGTTCTATCCATTATTTTATCTGGATAGTGTTAATTATTAATGTAGTTTTATTACTAGTTAGTTGCTTGTTGATGATTGCTGCTTATGCCCATCTTGATGGTTATAACATCTGAAGAAACTTAGAATCACTTAACCCTAATAACTAATGAAATCACTCTTTATTGGTTATTTTGATGGGTTACATCAAGGTCATCTATTTTTAAAGCAGAACAGTAAGTTTGAACCAATGGTGTTATTAATTGATAACCCACCTTTAAAACAAACCAACTGGCTTTATGATTTACAACAACGGGTTGCACAAATAAAAACTTACTTGAAAGCAACTGTAGAAGTATTTGATGTTGCCAAACATAACATGAATGCACTTAGTTTTTTTGAACAACAGATTAAAAGATTGAATTGTGATGAAATTATTGTTGGTACAGATTGGCATTTTGGTAATGATCATAAGGATGGGATCTGGTTAAAGAAACTGTTTAAAAATACTGTTATTGTTAATAAAACAAACCTATCAAGTAGTGTTATCCGTAACTATCTAACTAATAATGAACTTGAAAAAGCTAACCAACTTTTAGTGGAACCTTATTATAGAGTGGGCACAGTAGTACATGGTTTAAAAAAGGCAAGGTTGCTTGGTTTTCCAACTGCTAACATTGTTATGGATAACCACTTATTGACTTTAAATAAGGGGAGTTATATAGTAAGAGTTTTATTAAATAACCAAACTTTTTATGGGATTGGTTTTATTAGCCAAAAGGATCAGGATTTGGTGTGTGAAACCCATATCTTTAACTTTAATAATGAGATTTATGGTTCACTGGTCAAATTTACACTGTTAAAGTTCATTAGAACAATTAGTAAGTTTTCCAGTCAAGCAGCTTTGCAAAAAGCAATTCAAAGTGATGCTAACTTTGCTTTAAAGTGGTTGGAAAACCAAAATTTAGATAAAATTTAAAATCATCCAAATATGGACAGTGCCCCCAGTGGTTTAACTTTAACTGTTATTATCCTTAGCATCATTCTGCTTGCTTTTATCAGCACAGTTGTATCAGCTTATGAAACAGCAATCACTTCTTTAACCCCTTACAGGTGAAAGAACTATATCAAGACTAACAACAAGCAAGATAAACTATCAACTAAGATAATAAACCACTTTCAAAACCACTATTCAAGTTGTTTAATTACTATCCTAATTACTAACAACATAGTGGCCATTATGGTTTCTAACATCCTTTTTTTAGCACTAGAACAAACAATTAAAAATGAGCTTTTATCAAGTGTTTTAAATTTGGTAGTTAGTGGGGTTTTAATCGTCTCTTTTTGTGAAATTCTACCCAAAACTTTGGGCAGAATTAATGTGATTAGAACCCTGGTTCTATTTGCTTATTTGGTTTATTTTTTTTATCTGATCTTTTGACCAATTACCAAGCTAACCAGTTTAATTCTCAAAAAGTATGAAAACCCTTTACCTGTTTCAAGGAAAGATGTTTACTATTTTATTGATGAAATTGAACAAAACGGTTTATTTTCTAAAGAAGACAGTTTGTTAATTAAAAAAACCTTAATCTTTGACCAAGTACTAGTTAAAAAGGTAATGATCAAGTGAAAAAAAGTGGCTTATTGTTATCTTAATGACAGTATTAATTTGATTGCCAAGCAGTTTTTACAAGGGCAGTTTTCCAGAATGCCAGTAGTAGATAAAACTACTAATAAGATAGTTGGTTTTATCCATTTAAAGGATTTTTTTACAGCTAAAGAAGCAAACCCTAAGTCACTTGATTTAAACCAGTTGCTTTATCCAGTTGTTTTAGTTCAAGATTCCACCCCCATCAAACAAGCACTAAGACAGATGCGTTTAAACAGAGCACATTTAGCAGTTGTTAATGACAAACATGAAAAAACAATAGGGATTGTTTCTATGGAAGATATTATTGAAGAGTTGGTGGGTGAAATCTATGATGAACATGATGATATCCAACCGATCCAAGTATTGGATGAAAATGTTTGACTTGTTTTACCTAATGTAAAAGCAGCCTACTTTTTTAATAAATGAATTAAGCCAGATTTGGTAAAATCAAAAAATATTACTATCCAGCATTATCTCGCTTCACTAGATAATGATAGTTTTGCTTGCCAAAATAAGCTTGACACTCCCTTATTTAGTGTTGAAGTGATAGCTGATAGCGAAGATAAAACCAAAATTCTTTACGAAATTAGAAAAAAGAGCGATGTTATTGCTTAGAGCATTATTTTTAGAGTTGAAAACCAACAAAAATTGTAAAGCTTTACTTTTGTTATTAATCCTCTTACTAGTTGGTTTAACCTTGATAATCTATGGGATTGTCTTATTTTCCACTGAAGGGGTAATTGACCATGGTGATCATAACCACTTAAGAGCAAGGTTTCAACTCACTTTAGAGGAGATTATTGTTTTTGTTGTTGGTAGTATTATCTTGTTTTTTACTTTAGCTAGCTTTTGTGTAAGTTGCTTTATGTTAATGAGAAGTCCTAAGCAAAAACAGCTAGAGGTTGATCATGCTAATAAAACTAATTTAAAACCAAAAGCAATAGTTAATTGTGATCTTTTTCAGTTGGGTGATCATTGTGTGTTCACATTTAAAAAACTTAGCTTTAAACAACGGTTTAAGCAAGATTTTTTTGCTAGAAGTAAGTTTTCGTTTCGCAGTGAACTGTATCGTCTTTGTTTGGTGGGAGTGTCAATTGCTCTCAATTTAGCATTAAGTTTAATTGAAATTCCTGGGATAGTTTTACCTTGGGGTAGTTCGATCCAGTTCCGTTTTTTTAATACTGCTATCTTGTTTATTGCTGTTAGGTTTGTGGGGTTATTATCTACTTCTTTAGTGGCTTTAATCACCCCCTGATTACACCTGTTAATCCATCCTATTCACACCCCCATTAGTAGTTTGTTTTACATGGTTAATGACTTTTTGGTGCTGTGAATCTTTTATTTCTTTTATTTCCATCTCTTTAAAGCAGAGGTGATCCAAACTACTACTGTAGTTGACAACAAACCTTTTAGTCAGTTAGTTAATACTAAAAAAACGAAGTGAACAAAGTTTTTTCCTTACTTGCAATTAGCTTTTTGTGTGGCTTTATTGAAGGGTTAGGTTTTTACTTTGGTTATTTTTTAATCCTTGGTAATGTTTCTAGTTTAGGATTGAAGATCTATTATGATGGGTTACAACAACGTGATCTGATTAACAGTAGTAATGTTTTGTTCTTTTTAATGACAACAACTGCTATCTTCAGTATTAAATACATCTTTGAGATGTTATTTTTCTTTAGTGTTGAAAAGAATGTAGTTAATATTGCTAACCACTTTGGTTTGTATTAATCTAAACTTTTTGCAACTAATTAATTAGTTCACTAATAATGAAGTATGTAAAAGTACAGATCATCAATAAATCAACTATTGAACTGTTAGAGGATGCTAAAAAAGGGGAAAAATTAATTTAGATTTAATTAACCAAGTTGATCAGACCAACATCCTCAATACGATCACTACTAACCAAAAATTAGCATGAGAAAAGGAGTTAAGTGCTCAATTTATCAATCAGCAGAATGAGTTAATTAAAAACTTTGAAATTGAGATCATTAAGTTAAAAACTATGCTTAATGACAAAGAGCAAGCATTGTTGTTAAAAACCAAATTAGAATTACAAAACCAGTTTCAAAAGCAGATAGAGAACTATATCAATGAGATTAACAAGCTCAAGTTAACCAATAAAGAGCTTGAAATTACTAACCAAAAACAACTAGAAAGCTCACTTAAACTACAGCGAAACGAATTTGAAGAGAAAATCAACCAGCAAAACTTAACGATTGAAAAACTGAAAATTCAACAAGCAAGAAGTAGTATCTGAGCAGTTGCTAAAAAAGGAAATGAACTTGAAAAGTGGTGTGAAAACCAGTATGAGTCTTATGCAGATAGCTTTGAAAACTGTCAGTTTACTAGGTATAAAACTGAAATTAACTTATTAGATGAAAATGATTTTCCTAATGAAAAAGCAGATTACATCTTTAGTTTCTTTGGTGAAAAAACCAATAAAATTCCGTTTTTATCAATTTGCTGTGAGATGAAAAGTGAGTTTAATGATAGTAAGCATAAATCAAAAAACAAAGATCATATTAGCAAACTGGTCAGGGATGCTAAACGTGCTAACTGCAAGTATGCTTTTTTAATTAGTGAACTTGAACTGGAGACTGAAAATGACATCCAAGTGCGCTTAATGCCAACATTGGAAAGTGGTGTTGAAGTCTATCTAGTTAGACCAATGTTTTTTATCTTAATGCTTAAACTTTTCTATAAGTTAGCTAAGAAGTTGTTTGCCCTTAACCGTTTTCAATCAGTTGAACTAATTGATAAAAATAAGTTAAATGAACAGTTTAAACAGTTGAAAGATAATTTTTTAACCAAAACCTTTTTAGAGATTGAAAAAGTGTGTAAAAGTAACTTAGTTGATATTGAAACACTTGAAAAAGCAGTGGTGAAACTAAGGGTTAGAAACGAACGCGTTTTAGATCAGTTACTTAATAAATGAACTAAGAAAATTGATAGCTTTGATTTACAGTTAACTAAGAAAATTACTAATAACTACTAGGGTTTAATTTGTTAGCTTATTTAGAAAAATTCAAAATAAGCAAATTATAATTAGGTGTCTTTCTTTACTAAAAATATGAAATTATACCGATCTTTAAAAGCAGCCCTGTTACCAGGGATATGCACTAGCATTTTACTTGCTAGTTGTGCTTCAACAAATACTTATCAAGACCAAAGGAATGCCTTGATTAGTTTGGCTTCTAATCGTGATACTTTAATTGCGAATGCTAAAAAATCCAAAGAAGAAGTGCAAAAAGAAGTTACCAAAATGAATAGTAGTACTTCATCAATGATGACAGCTACCCAAAGTGTTGCAATTACAACACATCAAACTACTGCTCAAAACAGTTCCAAATATGATTTAGACAAGCTTTTTAAGGATTACATCCTTTATGTTGTTGATAATTTTTCAGGACTTGTTTTTAAAAGAACTGGCGATCATAGGATCCAGTTAATCGATAAGGATAAAGAGATTTTGGATGGTGGTAATCTAACTAAACATACCCACCATGATCATAACCATATGCATGAACATGAACATGAAGAACACCATGATGAAGAAGAGACAGAAGTAGTTGGCAGAGTACTCTCTTTTACTAATGGCATCTTTCTAGTGATTGACTATAAAAAAGACTCTGAAAGAAAAAATATGAGTGATTCAACTACTATGATGCACCAACACCACCATGAAGCTGAAGAACATAAAGAGGAACGTAAGCTCTCTTTAAACTTAAAAGCATACAAATTTAATACCCCTTTTAACATTAGTGAGTTTATTAGTGCTTGACATCATAAAGAATCTCATAATAGTGACACAGAGTTCAATAACCTTCACAATAAGTATGGCAAGGAAGAATTGGATATTATTGACTATAACTTTGAAGAAAAAGCTGTTGATGAAACAATTGCTTAACTAAAAAAGATAGTTATTAACTTAATTTAGAATTAACTAATGACAGTGTACACTTACACTGTTTTTTTATTTAATTGAGAAAATATTGGGTTGATGGAACAACAAAACCCTGATCGTTTAAAAAAAGATAGGGAACTTATTTATGCAATTGTTACAGCTAAAGGTATCATTAGCCGTTTCTTTTGATCAATCCTTAGTTTTTTAATTACTAACCTTATCTTCTTTTTTGCAGCTTTTGTAGCGCTCTTAATTTATCTGTTAGCAAGTGTTGGTAATCAGTTTGCATTTGCTTTTATTGCTGCAATTATCTTCATTATTTTTTACAACATCTTCTTTTTAAGTTACCTGTTGTTTATCTATTTTAAGGGCCAGAAAGCAATTGAAAACAACTGTAAGTACCTGTTAACAATCCTTGATATTAAGAGTGATGAACTGTTACCTTTTTCGCTTTTAGGTAGTTTAAGAAAAGGTTATATGCTAGATGAAATGCTCTTAGAACAGTAAATATTTGCTACAATCATAACGCTTTAGTTTTTAGTTGATACACCAAAATCCGTAGTCAATTTATTAACTAACTAGTGAACTAGATTTTGATGAATAGCGCTGTAAAATATCCTGAGCTGAAGATCAAACTTGAGTCTTATGATAGCACGCTTTTAGATCTCACTATTAAAAAGATAGTTGAGGTTGTAAAGGGTGTGAACATTAAGATTAAAGGTCCTTTACCTTTGCCTACTAAAAAGGAAGTGATCACCATTATCCGCTCTCCCCATGTTGATAAAGCATCCAGAGAGCAGTTTGAAAAAAATACCCACAAGCGCTTAATGATTCTTGTTGATGTTAATCAAGGAGGGATTGATAGTTTAAAAAAGATTAAGATCCCAGTTGGGGTTACACTGCGTTTTTCAAAATAGGTTATGGATGTAAGGGGAATATTTGGTGTTAAAGTAGGGATGAGTCAGATCTTTACTGAGCAAAATGAGTGCTTACCTATCACCATTGTTTATTGTGAAGCTAATCAGGTGGCTGGGATTAAAACGATTGCTAAAGATAATTACAACGCCACTCTATTAAGCTTTCAAACTGTTGATGAAAAACAACTTAACAAACCTAAACAAGGGTTCTTTTCCAAACTTAAACTAGAACCTCATAAATATCTGAGGGAAATCAGAAAGATGCAAGGGTTTGAGTTAGGTAAGAAGATCACCCCCCAGGAGTTGTTTAAGATAGGTGAATATGTTGATGTCACTTCACTCACCAAAGGTAGGGGTTTTACAGGAGCGATTAAAAGGTGAAACTTTAAGATAGGTCCTTTGGGTCATGGGGCGGGTTATCCCCACCGCTTTCAGGGTTCTGTGCAAGCAGGTAGAGGTGGTAGTAGTGCGCAGCGTGTTTTTAAGGGTAAGAAGATGTCTGGGCATTATGGTCATGAACAAGTTACGATCCAAAACCTCTTTATTGTTGGCTTTGATGAAATCAATAAGTTAGTGTTAGTTTCAGGCGCAATTGCTGGTCCTGAGGGTGGGATTGTTTTAATTAAAACTGCAAGAAAGAAAACTGGCAAGATAAAAGATATAAAGTTAGCAGTACAAACTGTTAAAGCCCCACAACTAAAAGCACCAAAAAAGCAGAAAACTAAGGTTGAAACCAACCAGGTTAACCCAAAAATTGAAGAAGAGAAAACTAAGTAATGGCTAAGCTTAAAGTAATCCAGTTTGATGGTAGTTTTAAAGGTGAGATCCAACCTGCTAACCACCTCCTTTTAAAAAAAGCAGTGATCCAACCAGTGTTTGATGCTATCTTATTAGAACAAGCAGCATGTAGACAAGGCACTCACTCCACTTTAACTAAGGGTGAAGTTAGTGGTGGGGGTAAAAAACCATATAAACAAAAGCACACTGGTAAAGCTAGACAAGGTTCAATAAGAAACCCCCATTATGTGGGGGGAGGTGTTGTTTTTGGTCCTAAACCCAACCGTAACTACAAACTAAAACTAAACAAAAAGGCTTATCAACTTGCTTTAACTAGTGCCTTTGCACAAAAGCTTAACAACAACCAAGTGATAGTTGCTGAAGCCAAGTTGTTTGAACAAACCAATGCCAAAACTAAAAAGATGCTGACGTTTCTCAAGAATGCCAAACTAACTGAGCAAAAACTCTTGTTTGTGATTGATACTATCTCAAAACCACTGTTGTTGAGTACTAACAACCTAAAGCAGATAGTAGTCAAACAGTTTAATAAAGTATCAGTAAGAGATCTACTTTTAGCTAAAACTATCATCATTGAAAAAGCTGCTTTTACAAAACTGGAGGAACGACTTAAATAGGCTATGGATGTAACCAACATACTCTTAAAACCAGTCTTAACTGAAAAGAGTTATCTCAACCAGATGGGGGAATTGAAAAAATATGTCTTTGCAATTAACCCTAAAGCTACTAAAACCAAAGTAAAACTAGCGTTTGAAATTATCTATGGGGTTAAACCTTTAAAGATTAACACGCTAATTAGAAAACCAGTGACCATTAGAAATGGCACTAAATACCCTGGGTTTAGTAAGCTAGCAAAACTAGCAGTAATCACCTTACCTAAGGGAATGGATATTGCCATTACTGGTGAGAAAACAACCAAGAAAGAAACAAAGGATCAATAATGGCAATTAAAAAGATTATTAGTCGTTCTAACAGTGGGATTCACAACGCCACTGTCATTGACTTTAAAAAACTCCTTACCAATTCCAAACCCGAAAAGTCGCTTTTAGTTACTTTAAAAAAACATGCAGGAAGAAACAACCAGGGCAAGATCACTGTTCGCCACCACGGTGGGAGACATAAACGTAAGTACCGTTTAATTGATTTTAAGCGTTACCACTATGACAATTTAAAAGCAACTGTTAAATCGATTGAATATGATCCTAACCGCAGTTGTTTTATCTCCCTTTTACACTATCAGAATGGGGTTAAAACTTACATCATTAGTCCTGATGGGATTAAGGTTGGTGATCAAGTTTATTCATCTGATCATGCCATTGATATCAAACTAGGTTATTGTATGCCCCTTGCTTTTATCCCTGAAGGAACCCAAGTTCATAACATTGAACTTAACCCTAAGGGTGGGGGTAAGATAGCAAGAAGTGCTGGAAGTTATGCGAGGATCTTGGGTCAAGATGAGACTGGTAAATACATCATTCTCCAGTTAATCTCAGGGGAAACTAGGAAGTTTTTAAAGGAGTGTAGAGCTACAGTTGGTGTTGTCTCTAACTTAGATCATAACCTTGTTGTAATTGGTAAAGCAGGGAGAAGTCGTCATAAGGGAATCAGACCAACGGTTAGAGGTTCAGCAATGAACCCTAATGACCACCCGCATGGGGGTGGGGAAGGGAGAAGCCCAGTTGGCAGAGATGCACCAAGAACCCCTTGGGGCAAACGCCATATGGGTGTGAAAACACGTAACATGAAAAAACATTCAACTAACCTGATTATTAGAAACAGAAAAGGAGAACAATACTAATGTCAAGAAGTAGTAAAAAGGGCGCATTTGTTGATGCTCACCTCTTAAAAAAAGTGATTGAAATGAACAAACAAGCCAAGAAAAAACCAATTAAGACTTGGTCAAGAAGAAGTACTATCTTCCCTGAGTTTGTGGGTAACACCTTCAGTGTGCATAACGGTAAAACCTTTATTAATGTTTATGTTACTGATGATATGGTAGGTCATAAGTTGGGTGAGTTTTCCCCAACTAGAAACTTTAAACAACACACTGCTAACCGTTAGTTATGATTGCTTTTGCTAAACAATACAGAGTTCACATCTCCCCCCAAAAAGCACGTTTAGTGTGCCAGTTAATTGTGGGTAAAAAGATTAATGATGCGCAAAACATCCTTTTAAATACGCCAAAGAAAGCTGCTTACTTTTTAACTAAGTTACTAAATAGTGCGATTAGTAATGCCACTAATAACCACGGGATGAGCGGGGATCTTTTGTATGTATTTGAATGTGTTGCTAACCAAGGACCTAGCATGAAAAGAACAATCGCTAGAGCCAAAGGTTCAGGGAGTGTTTTAACCAAGCGTTCTTCAAACCTAGTTATTAAGTTATCTGATAATCCCAATGAAAGAAAATTACTCTTAACCCAACAAAAGGAACTGGTGAAAAAAAGAACAATGGGTCATAAAAAAGAGAAAGCAAAGCAAAAGCAAAAACAACAATAACTATGGGACAAAAAGTAAATTCAAACGGCTTAAGGTTTGGCATTAATAAGAACTGGATCTCACGGTGAACTGCCAGTTCCAACCAACAAACAGCAACCTGATTAGTACAAGATGAGAAGATCCGTAACCTCTTTTTTATCAACTATCGCAACGCTCAGGTGTCTAATGTTGAGATAGAAAGAACCCAAACGACTGTTGATGTTTATGTCTATGCAGCTCAACCTGCTTTATTGATAGGCAGTGAAAACAAAAACATCCAAAAGATTACCAAAATGATCCAAATCATTGTGGGCAGAAAGATTAAACTTGATCTTACTATCAATGAGATCGGCTCTCCGATGTTATCAAGTAGGATCATTGCCCGTGATATTGCTAATGCGATTGAAAACAGAGTACCACTCCGTTCAGCAATGCGCCAAGCTCTAACCAAGGTTTTAAAAGCAGGTGCTAATGGGATTAAGGTATTGGTATCAGGCAGATTAAATGGGGCGGAAATTGCCCGTGACAAGATGTATATTGAGGGCAATATGCCTCTTTCAACTTTAAGAGCAGATATTGACTATGCCTTTGAAAAAGCAAAAACCACCTATGGCATTATTGGGGTGAAAGTATGGATTAACAGGGGGATGATCTATGCAAAGGGTTTAAACAGAACCCCAGCACACATCCTCCATCCCCAAAAGAAACAGCTAAAAACCCCAACTATCAAAAAAACCAATTCAGTAATAGCAAAACAAAAACTCACTGGTAGTGATATTGAAACTGCTAGTTTAAAAGCACTTACTGATAATAATCAAAACCACGAATAGTTAAGATGTTACAACCAAAAAGAACCAAATACAGAAAACCACATAACGTCAGTTATGAAGGACACACTAAGGGCAATGGTTATGTTGCTTTTGGTGAGTATGGAATTGTTGCTACTAAGGGTAATTGGATCGATGCGAGAGCAATTGAATCAGCGCGGGTTGCTATCTCAAAGTGCTTGGGTAAAACTGGAAAGATGTGAATCAGGATCTTCCCCCACATGTCAAAAACCAAAAAACCCTTAGAAGTGAGGATGGGTTCAGGGAAAGGTAACCCTGAATTTTGGGTTGCTGTTGTTAAAAAGGGGACAGTGATGTTTGAAGTTGCTAACATCCCTGAACAACAGATGATCAAAGCCTTAACAAGAGCAGGCCATAAACTCCCTGTTACCTGAAAACTAATGAAAAGAGAGGAGAACAGTTAATGACAATCGCTAAGGAGCTGAAGCAAAAGAGCAACGAAGAGTTAGTGAAACTAGTAATTAAGCTTAAGGGTGAACTCTTAGAATACCGCTTTAAACTTGCCCATGGTGAACTTGACAAACCCCATCTGATTGCCAAGGTGAGAAAGTTATTAGCAGTTGTACTTACTATTCTCACTGAACGCAAACTCAACTGACAAGTTGAAAAAGATAAGTACAAGTTACTTTCAAGAAAAACCAATGAACTTATTGTTAACAGTTGAAAGCAAAAACTATCAACTAAACCTGAATCCAAACAAGAAACTAAAAAGGCTGAAGTTAAACCTAAGGTTGAATCAAAGCCTGAATCCAAACAAGAAACTAAAAAGGCTGAAGTTAAACCTTTAAAACAAGAAACTAAAAAAGTTGAAGTTAAACCTAAAGTTGAACCAAAACCTTTAAAACAAGAAACTAAAAAGGTTGAAGCTAGGATTGAAACTAAGACTAAAGTTGAATCAAAACCTTTAAAACAAGAAGTTAAAAAGGTTGAAGCTAAAAAATCTGTTTCAAAACCCCAAAAACCAGTTAAAGCCAAAATGATTAAAACAAAGGAGAAAAAACAATAATGAAGCGCAACCAACGTAAGCAGTTAATTGGCACAGTTGTTAGCACCAAAAATGCTAAAACAGCAACTGTCAAAGTAACATCACGCTTTAAACATCCTTTGTATCACAAATCAGTTATTCGCCATAAAAAGTACCATGTCCATAACTTTGGTGAACTTGTTGCTAATGATGGTGATAGGGTACAAATTATTGAAACAAGACCCCTTTCCGCTTTAAAGCGGTGAAGGATTGTCAAAATCATTGAAAGAGCAAAATAGTTTATGGTTAGTTTTATGACAAGATTAAATGTAGCTGATAATACAGGCGCTAAGCAAGTAGGTATTATCAAAGTTTTAGGTGCTACATACAAACGTTATGCATTCCTTGGTGATGTTGTTGTTGTATCAGTTAAAGATGCAATCCCTAATGGCATGGTTAAAAAGGGTCAAGTGTTAAGAGCAGTCATTGTTAGAACCAAAAAGGGACAACAACGCCAAGATGGTACCCACCTAAAGTTCCATGACAATGCTTGTGTGCTTATCAAAGAAGATAAATCCCCAAGGGGAACAAGAATCTTTGGGCCAGTTGCTAGGGAGTTGAGAGAAAAAGGTTACAACAAGATTTTAAGCTTGGCGGTGGAGGTTGTTTAATGCAAAGGATTAGAAAAGGTGATAAGGTAGTTGTGATCACTGGTAAAAACAAGGGCGGTAGTGGGATAGTGCTTAAGGTATTAACCAAGCAAAACAAAGCGATTGTTGAGGGGATCAATAAGGTTACTGTTCACAAAAAAGAACAAGTCAACAAGCACAACAAACAAACAAACCCAACTACTAAAGAAGCCCCTTTACCATTAAATAAACTTGCTTTATTTGATCAGAAGGCCAAACAGCAAACAATTGGCAAGATCAAATACCAAATTGATCCTAAAACCAAACAAAAAACAAGAGTCTTTAAAAAGACTAATAATGCCATTTAACTGTTATGAATAACCTTGAAAAAACCTATAAAACTGCGTTAGTTAATCAACTCCAACAACAGTTGGGCTTTTCTTCCATCATGCAAGTCCCTAAGTTAACAAAAATCGTTGTTAACATGGGAGTTGGGGATGCAATTAGAGACAACAAGTTCCTTGAATCAGCACTAAATGAACTGCACCTGATTACTGGTCAAAAACCTGTTGCTACTAAAGCTAAGAATGCTATCTCAACTTACAAGTTACGTGCTGGTCAATTAATTGGTTGTAAAGTTACTCTAAGAAATAAAAAGATGTGATCCTTTCTGGAAAAATTAATCTATATTGCTCTGCCCAGAGTAAGGGACTTTCGCGGTTTATCACTGCGCTCTTTTGATGGGAAAGGTAACTATACGATTGGCATTAAAGAACAGATTATCTTCCCTGAAATTGTCTATGATGATATCAAAAGAATTAGGGGTTTTGACATCACTATTGTCACTTCCACCAACAAAGATAGTGAAGCACTTGCTTTACTGAGAGCACTAAAGATGCCGTTTGTAAAAGAATAGATATGGCTAAAAAATCATTAAAAGTAAAACAATCCCGTCCCAATAAGTTTAGTGTACGCGACTACACCAGGTGTTTAAGGTGTGGGCGTGCTAGAGCAGTGTTAAGCCACTTTGGTGTGTGTAGGTTGTGTTTCCGTGAACTTGCTTATGCAGGAGCAATCCCAGGAGTTAAAAAAGCATCATGATAATCAATAAAGTTCCCAAAGCCCATTTTGATCCAGTTTCTGATCTTTTCACTAAGATCAACAATGCTAGAAAAGCTAAGCTTTTAACTGTTACCACCATCGCTTCTAAGTTAAAGATAGCTATCTTAGAGATTTTGATTAAAGAGGGCTATTTAGCTAACTATCAGGTGTTGGAAAATAAAACTAAAACCAAAAAACTAGTTAGTTTCACATTAAAATACACCCAAAGAAGGATATGTTCTATTAATGGGGTGAAACAGATCTCAAAACCAGGATTAAGAATCTATCGTTCCTTTGAAAAACTTCCCCTTGTTTTAAATGGTCTTGGTATTGCAATTATCTCCACTAGTGATGGAGTGATGACTGATAAAGTAGCAAGGTTAAAGAAGATTGGTGGGGAGATTTTAGCTTACGTTTGGTAAAAAATTATGTCAAAAATAGGAAATAGACCAATCAAAATTGATCCTAGTAAAGTGAGTTTAATGCAAACAACAACACTGCTTACTATTAAAGGACCATTAGGGGAAAACACCATTAAACTACCCAAAAACTTACCCTTAAAGTTTGTTGTTGAAAATGACACTATTAAAGTAACTAATAACAACAACTTAAAACAAACTAAGATCTTACACGGTACTTTCAATGCGTTAGTTAACAACGCAGTTATTGGGGTTACCAAGGGTTTTGAAAAGAAACTCATCCTAGTTGGGGTTGGTTATCGTGCTAATGTGGAAGGGCAATTTCTCAACTTACAGTTGGGCTATTCCCATCCTATTAAGGAGTTGATCCCAAACCAACTTACTGTTAAAGTAGAGAAGAACACTGAAATCACTATTAGTGGAATAAAAAAAGAGTTAGTAGGTCAGTTTGCCACTGAAATCAGAAAGTGAAGAAAACCTGAACCTTATAAAGGTAAAGGGGTACTTTACTTTAACGAAGTAATTGTTAGAAAACAAGGTAAAACTGCAGAGGGCAAGAAATAAGATGACAAGAAACGATAAAAGAAGGATTAGACACAAACGGATTGTCAAAAAAATTAGGTTAACTAACCTTAACAACAGGGTTGTACTAATTGTTATTAAGAGTTTAAAAAACATCTCGGTTCAAGCTTGGGACTTTAGTAAGAACGTTGTTTTAACATCAAGTTCCTCACTTCAACTAAAATTAAAAAATGGCAACAAGGAGAATGCTAAACTAGTGGGAATGGATATTGCAACCAAACTCATCAAACTAAACCACAAGGATGTGGTTTTTGATACTGGGGGTAGTAAGTACCATGGTAGGATTGCTGCTTTAGCAGAAGGAGCGCGAGCTAAGGGTTTAAATTTTTAAAGTTATGAATGATCCAAAAACTACTAACACTGGCTTGTTAACTTCCACTCTTAAAACCAAGCCCAAACACAACCTTAAACCTTCCAGTGAAGCCATTAAAAAAGCAGTGTCCAAAAAGGAAGGTCACTACAAAAACAAGCGCTTTCAAAAACATAACTTTAATAACAAAAGTGAGTTTGAAGAGAGGATTGTCAAACTTAAACGGATCTCCAAAACCACAAAAGGCGGAAGAAACATGCGCTTTAGTGTCCTTGTTGTTGTTGGTAACAAAAAGGGCAAGGTTGGTTATGGGATTGCTAAGGCATTGGAAGTACCACTTGCCATTAAAAAAGCGATTAAAAAAGCCCATAACTCCATCCATACAGTAGAGATCCACAAGGGCTCGATCTACCACGAAGTGATTGGTAGAAAAGGTGCATCCAAGGTGTTGTTAAAACCTGCACCTTTAGGAACTGGGATCATTGCTGGGGGAGCGATCCGTGCAATTGTAGAGTTAGCTGGTTTTAGTGATATCTATACCAAGAACTTGGGAAGAAACACCCCCATTAACATGATCCATGCCACTATGGATGGGATCTTAAAGCAACTCTCACCCAAAAAAGTGGCATTATTAAGAAATAAACCAATTAGTGATCTATAAAAACAATGGAACTACACCAATTAAAAAGTGTCTCTAAAAGCCGTAACCACAAGTCCAAAGTGGTAGGTAGGGGCCATGGCTCGGGATTAGGTAAAACATCATCACGTGGTCAAAAGGGACAAAAAGCAAGAAAATCAGGTTTAACTAGGTTAGGTTTTGAAGGGGGACAAACACCCCTTTACCGCCGGTTACCTAAGTATGGGGTTGCTAACAAGGGGATCTTAAAAAAAAGGTGGGTTGTTTTAAATTTGAACAAAGTTGCTAAACTCAATCTCAAAACAGTTACTAGAGCAACTTTGATTGAAAAAAAGGTAATTAGTAAAAAAAATAACCTCCCTTTAAAGTTAATTGGGAACACAAAACTCACTACTCCCATCCACTTTGAAGTGCAAAAAATCTCCAAAAATGCTTTAAATGCAGTGCAAACTAGCAAAGGTAGTGTGAAAATTATCACCTAATGCAAACTGTTTCTTCACCCAAACAAAAACTTAACTTTGGTCAAAGGTTACTAACTCTATTACAAAACCGTGACTTTATGGTGTCACTGGTTTTAACAGTGGTACTTTTAATCTTGTTTAGGGTGTTAGCAATTATCCCCTTACCAGGGATTAGGATTAATGAGAGTGTCTTGGATAGAAATTCCAATGACTTTTTCTCCCTTTTTAACTTACTTGGGGGTGGGGGATTAAACCAGTTATCGTTGTTTGCAGTTGGGATCAGTCCTTATATCTCAGCCCAAATCATCATGCAACTGCTTTCAACTGATCTAATTCCTCCACTTTCAAAACTAGTTAACAGTGGGGAAGTGGGGCGAAGAAAGATTGAGATGATCACAAGAATTATCACCTTACCCTTTGCTTTAGTGCAAGCATTTGCTGTGATCCAAATTGCTACTAATGCAAGCACTGGTTCAAGTCCGATTAGTTTAGCTAATAGTGGCAGTGAGTTTATTGCTTTTTATATTATTACTATGACTGCAGGGACTTATATGGCAGTGTTTTTGGGTGATACTATCTCCAAAAAGGGGGTTGGTAATGGGATTACTCTGTTAATTCTTTCAGGGATTTTATCCCAACTCCCCCAGGGCTTTATTGCTGCTTACAATGTTTTGAGTGGGATAGTAATTACTTTAACCCCACAGTTAACTGCAGCAATTAGCTTCTTTATCTATTTCTTAGCATTCTTAGTTTTACTGTTTGCCACTACCTTTATCACCCAAGCGACCAGAAAGATTCCCATCCAACAATCAGGACAAGGGTTGGTTAGTGAAGTCAAAACCTTACCTTATTTGCCTATTAAGGTGAATGCTGCTGGGGTGATCCCTGTCATCTTTGCATCCAGTATTATGTCTATCCCTGTGACCATTGCCCAGTTTCAACCCCAAACTGAGTCACGGTGGTTTGTGGAGGATTACCTATCACTTTCAACACCCGTAGGGATCTTTTTATATGCAGTTTTGGTTATCCTTTTTCCTTTTTTTACAGTTACATCCAGATTAACCCAGAACGGTTAGCTAAGAACTTTGAAAAATCTGGCAGATTTATCCCAGGGATTCGACCGGGCAATGATACAGAGAAACACATTGCGCGGGTGTTAATAAGGATTAACTTTATAGGTGCTCCTTTTTTAACTGTTATTGCTATTATCCCTTACATTGTTTCTTATTTCATTAGGTTACCTAACTCCTTGAGTTTAGGGGGGACGGGGATTATTATTATTGTTACTGCTGTAGTTGAATTTATCAGTGCACTGCGTTCAGCTGCTACTGCTACTAACTACCAACAACTAAGGAGAAACTTAGCAATTGAAGTGCAACAAACAGCTAAACAAGATAGTCTAGAGCAGCTTCAAAAAGAAGCACCAGGGATTGGTAACCTATGGTAGCACAGTTTAATAAGTTCATTATCTTAGGACCCCCAGGGGCAGGAAAAGGTACAGTTTGTAAACTGCTTAGCAAAACAACTAAGTTAGTCCATATTGCTAGTGGTGATCTGTTTAGAGAAGCCATTAAAAACCAGAGTGCTGTTGGTAGAAAGATTGCAGCAATTATCAGTCACGGTGGTTATGTTGATGATACCACTACTAACCAGCTTGTTTATGAATATATCACTACGAATCCATTACCAAATGGTTTTATCTTAGATGGTTATCCAAGAACAGAGAACCAGCTTGATTTTCTAAATATTAAACTAACCATTGACATGGTCTTTGAACTAGTTGTTAGTGATCTGAATAAACTGATTACACGGATTGATAACAGGGTTATTTGTAACAACTGTAACAGTGTTTATAACTTGCTTTTTCAAAAACCACTAGTTGAAAATAGTTGTGATCAGTGTTCAGCTAAACTAGTGAAAAGGAGTGATGATAACAAAGCAGTGGTCAAAGCAAGAATGGAGTTATATCAACAAACAATTCAACCAATCCACACTTACTTTTTCAACAAACAACTTTTAGTACAAATTGATTGCTTTTTACCACTAGAAGAACAACTCAAGACAATCAAACAATTTATTAGATAAAGATGATCTATCTCAAATCTGCAAATGAAGTTGCAGGGATTAAAAAAGCATGTGCAATCTTCAAAGCAGTTAAGGCATATTTTACAATTGAAAAGTTACTTGGCAAAAAGTTGGTTACCATTGATCGTTTAATCAAACAATTCATTGAACAAAAACAAGCTAAATGTGCGTTTCATGGTTATCTAGGTTTCCCTGGTTTTAACTGTCTATCGTTAAACCAAACGGTTATCCATGGAGTTGCCGATCAAACTGTTTTTAAAGATAGTGATAAACTAACGCTTGACATTGGGATAGACTATCATGGTTATCTTTGTGATGCAGCTTTCACTTTACTTGGTAGTAAAGCTGATCCAAAGGCAGTAAAACTGTTAAATGATGTTGAACAAGCATTTAGTAAGGTAATTGAACCTGAGCTATTTGTTAACAACCGAATTGGTAATTTATCCAATGCGATCCAAACTTACTTTGAAAACAAGGGCTATTTTCTTGTCAAAGAGTTTGGGGGTCATGGTTGTGGGATTAAGATCCATGAAGATCCTTTAATCTTAAACTGGGGAGAGAAAAACCAGGGCATTAGGTTACAAGAGGGGATGGTAATCTGTATTGAACCGATGGTTATGACTGATAGTAGTGAGATAACAATGGCAGCTAACAACTGGAATGTACTAACTTTAAAAAGTAAGTTTAACTGTCATGTGGAACAGATGTATCACATCACAAACAACGGCTTTGAATGTTTAACTAACTAATGAAAAACGATAAACTCTTTCTAACAGGTAAGATACTGGAAATTATCCATGGTGATAAGTACCGGGTGATGCTTGAAAACAATGTTGAGGTTGATGCACATCTAGCAGGTAAAATGAAGATGAAAAGAACCAAGATTCTCCCTGGGGATGTTGTTGAGGTGGAATTTTCTCCCTATGATTTGAAACTAGGTAGGATAACCCAAAGAAAATAATTAAAATATTATGAAGGTTAGAGCAAGCGTAAAACCAATTTGTAAAGATTGTAAGATCGTCAAACGTCACCGCATCTTAAGGGTGATCTGCAAAACCAAAAAACACAAGCAAAGGCAAGGATAATGGCACGAATCTTAGGGATTGATATCCCCAACCAAAAACGGATCGAGATAGCTTTAACATACATCTTTGGGATTGGTTTGTCAAGTGCAAAAACAATCTTAAAAAAAGCAAAGATTAACCCTGATAAACGCGTTAAAGATCTGAGTGAAGAGGAACTTGTTGCGATTAGAAACGCAGCAAGCGGTTACAAGATTGAGGGTGATTTGAGAAGAGAGATTGCTTTAAACATCAAACACCTAACAGAGATCGGTTCTTGAAAAGGGATTAGACACAGAAAAAACCTGCCAGTAAGAGGACAACGCACTAGAACCAACGCAAGAACCAGAAAAGGCCCTAGAAAAACAGTGGCTAACAAGAAAATTGAAAGTAAGTAATGGCTAAGAAAAAAAAGATTAATGTTCCCAGTGGTTTGATCCATGTCTCCTGTTCACCTAACAATACCATAGTATCAGCCACTGATCCCAGTGGTAATGTCTTGTGCTGAGCGAGCAGTGGTACAGTAGGATTCAAAGGTTTTAGAAAGAAAACCCCTTACTCAGCAGGGGTAGCAGCTGATAAGGTGGCTAAAACTGTGAAAGAGATGGGAATGGGGAGTGTTAAGATGTATCTGAAGGGAACAGGTAGAGGAAAAGACACCACGATTAGAAGCTTTGCTAATGCTGGGATTACGATCACAGAAATCAATGAAAAAACCCCTATTCCCCACAATGGCTGCAAGCCTCCTAAGCGTCCGCGCTAATCAAAACAAAACAACTTATGGAAAAATTTTTAAAGTACGAAATTAAGGTTAACAACAACCAACCAACCAACACTAACCCCAACTATGGGATCTTTGAAGTAGCACCGTTAGAATCAGGGTTTGGAATTACCATTGGTAATGCGATGCGCCGAGTGTTACTTAGTTGTATCCCAGGTGCTAGTGTGTTTGCCATTGCCATTAGTGGGGTGAAACAAGAGTTTAGTAATGTGGAGGGTGTTTTGGAAGATGTGACTGAAATGGTGTTAAACTTCAAGCAACTGGTGGTGAGAATCTCTGATCTTTTGTTTGAAGATGGGGAGATGATCGAACCACCCTTAGAAAGGTGACCAGTTTTAAAAGTTACTGCTGAAAAAAAGGGTGCAGTATATGCAAAGGATCTGGAGTGTCCAGCTGGTTTTGAAGTGATTAATAAGGACCTTTATCTCTTCTCTTTACAAAAGGACATGAAACTAACAGTCAGTGTTTATGTTAAACAGGGTAGGGGCTTTACTAGCTTTCTTGAAAACAGAGAATTGATCAATTCGCTTGGCATTATTGCTACAGATGCTAACTTTTCCCCGGTTTTACACTGTGGTTATGAAGTTCAAGAGGTGAAAACTTCCAAACAAAAGTTAACTGACCATCTCACCTTTAAGATTGCTACTAACGGTGCAATTAAAGCAGTGGATGCGTTTGCTATGGCAGCAAAGATCCTAATTGAACACTTAAACCCAATTGTAAGTGTCAATGAGTCAATTAAGAATTTAACAATTATCCAAGAGAAAGCAGAGGAAAGAAAGGTGAAATCATTTGCCAAGCAAATTGAAGAACTTGACTTTACTGTTAGAACCTTTAACTGTTTGAAAAGAAGTGGGATCCACACACTCCAAGAGTTACTATCAAAGTCATTAACTGACATTAGAGAGATTAGAAACCTAGGTAAGAAATCAGAACGGGAGATTATCAAAAAGGTGCAAGAGTTAGGTTTAAAATTCCGTTTTTAATAAAATAGAGCTATGTCATACATTAATAAAGAGGGGAAAACCACAGCTTGAAGAGTGATGACAGTGCGTCAGCAAGTGAGTGCAGTGTTAAGTTATGGAAAGATTCAAACCACTTTAAAAAAAGCTAAGAACACCCAAAAAAGGTTAGAGAAGATTATTACCATTGCTAAAGTTGATAACTTTAACAACCGCAGGGCTGTTAAAAAGTGGTTATTAAATACCAATTCATTAGATGTAGATCAACTCACAAACCACCTTTTTAAAAAAGTAGCGCCACGTTTTTTAAAGCGTAATGGTGGTTATAGTAGAGTGTTAAAGTTGGGAGTTAGAAGGGGTGATAGTACTGAAATGGCGATCTTACAGCTGATAGATGCTACCAACTAAACAAGCTGCTTGTAGTTTTATTAATGTTGCTTTTTCCTATAATGAACTGCCATTAATTAGGGAACTATCTTTTAGTGTTTATGAGGGGGAATATGTTTGTATTGTTGGTCATAATGGCAGTGGTAAATCAACCATTTCCAAACTGTTAACAGGGTTATTAAAGCCCCAGGCAGGTGAGATTAAGATCTTTGGTAAAACAGTTGATTTTGATAATGTTAGTTACTTGAGAAATAACATTGGGATCATCTTTCAAAACCCTGATAACCAGTTTATTGGGATCACTGTTGAAGATGACATTGCCTTTGGGCTTGAAAACAAGTGTTTTTCAAGACAGAAGATAAAAGCCATTATTGATGAAGTTACCCTACAAACCCAAACTGATGGGTTTATTAAACAAGAACCCCATAACCTATCAGGGGGACAAAAACAACGGGTAGCAATTGCATCTGTTTTAGCACTAAATCCTGCTATTATCATCTTTGATGAATCAACTGCGATGTTAGATCCTAAAGCTAAAAAAACGATTAAGCAGTTTATGGTTAAACTAGCCAAACAGGGCAAGTGTGTGATCTCAATTACCCATGATATGGAAGAAGTTACTAAAGCTGATAAGGTGTTAGTAATGAATGAGGGCAAACTGATCAAACAAGGTAAACCTGTTGAAGTTTTCACTAGTGAACAAGAGTTACAAAAAATCCGTTTAGACATCCCTTTTTCACTCAGTCTTTCAACCAAGATAAAAGGGATCACTAGTACAATTGATTACCAAACCCTGATTAAATCAATTGCCAAGCTGTGAAAAAAAAGATAGTCCCAATTAACCCTTTAAAAGCAGATGAGATTTTAGCAGTTAGTCACTTATCATGTGTTTTTAACAGTAAAACTAACAATCCCATTAAGGTGATTGATGATTTTTCCTATACGTTTCAAAAGAACCAAATTTACTGTATTATTGGTGATAGTGGCAGTGGTAAATCAACCCTTGTTAACCACTTCAATGGGTTGATAAAACCCAACCAAGGTGATATTTGGGTTAAAGATATCTATATTGGTGCTAAACAACGCAAGATTAAGAACTTTAAAAAACTGCGAAAAACTATCTCAATTGTTTTCCAGTTTCCTGAGTACCAATTGTTTAAAGATACCGTGGAAAAAGACATTATGTTTGGTCCAGTAGCATTAGGTCAATCCAAGTATGATGCGCGCCAAAAAGCGGCTTATTATCTGGAGATGATGGGGTTAAAATACCCCTTTTTAGAACGTAATCCCTTTGAATTGAGTGGGGGGCAGAAAAGAAGGGTAGCGATTGCTGGTATACTTGCAATTGAACCAGAAATTCTAATCTTTGATGAACCAACTGCTGGGCTTGATCCTGAAGGGGAAAGGGAGATGATGCAGTTAATTAAAACTGCCAAACAACAACAAAGAACGGTATTTATGATCACCCACCAGATGGAAAATGTCCTTGAGGTGGCTGATGTGGTTTTGGTTTTAGCTAAGGGTAAACTAGTAAAAGCTGCTAGTCCATATGAAGTGTTTATGGACCAAACTTTCCTTGAAAAAACAACGATTGTTCTCCCCCCTGTGATCCAAGTGATCAAAGATCTAATTGCGATTAATGCTCACTTTAATAAGTTAATTGAGTTGCAACCAAAGAATCTAGAACAGCTTGCATCAGCAATTAACAAGACTATAGCAAACCATGGATAACTTTATTAATGGCTATATCCCAAGAAACAGCTTTGTTCACAAGCTGCATCCAACTACTAAACTAGTAATCTTTTTACTGTTAGTTATCTTGGTATTTGTACCAATTGGCTTTGTTTTTCAAAGTGTTATCTTTCTTTTTGTTACCTTTGTTTTTTTTATTGCTAAACTCCCGGGGCGGTTTTACAGTTCAGCAATTAAGTCAATTACGCTGTTATTTCTCTTGTTGTTATTTGTAAACTGGTTTACCTTTCGTGATCCAGGGTTTTATCTTACTAGTGATCAACTTAACAGTTTACCAGCCATTGATAACAGCAAGTTTAGCTTTTGAAACATTAGTTTGTTTAACTATCAAGATAATGTTTTTTCCCAGGTTTTTGCTTTTAACAGGGGTAATTTAACCAACTTAAATCAACTTGATTTTTTCTATAAAGCTAACAATGCTGATAGTTACACCAAAGTAAAGGGCATTGATAGTTTAGCAAGTATGCTAGCAAACAATGGCAATGGTTTAAGCAAAGACAAAATTCTGAGTGCTTTTTTAGATCACAATTTAAACCTTTATTTAGCGAGAAGTTGGGGGGCAAATTTTGCTGGGTTTGTTGTTGATTTTAACCCAACAACCCAACTCTTTAAACTCACTCCTTTTCTAGCAAATGCTAGTTATGTTTTAACGTTAAGAGCAGTTATCTTAGCATTCTATGTCACCCAAAAGATCCTAATTATGATCTTATTTGCAACTGTACTCACTTCCACTTCAAGTTCAGTTGAACTAGCATATGGGATTGAAAGGTTATTATGACCTTTAAAACTCATTAAAATACCTGTTAATGTCTTTGCAATGACCATTGCCATTGCCATTAGGTTTGTCCCTTCCTTGTTACTAGAATCACAACGGATCTTAAATGCCCAAGCATCCAGGGGTTTAGACTTTCGAAATGGGGGATTTTTAGTGAAGATGCGTTCACTCTCTTCGTTAGTAGTACCAATGGTCTCCATTGCATTTAGGAATGCCTCTGAACTTGCTAGTGCAATGGAAGCAAGGGGTTATCACCCTGCAAAGAAGCGCAGTAGTTATAGACAATACAAAATCACTTGGATTGATATTTTAGCGTTATTTTTGGTTTTTGCTTGGTTTGTTGTGATTATCTTTTTAACTATTAGAGGAGCGGTCTTTTTGGATATAGGTACACCAGAATGGTTACTAACAGGAAAGATTAATGAACAGGTAATCAGGGATCTGAAGGTAAGTGGCTAGGTACTTGGGGATTGTTAGTTATGATGGCAGTTACTTTAAAGGGTGAGCGATTCAACCAAACCTAGCTACTATCCAAGATTTATTGGAACAAAGTTTTTCATTAATCATTGGCAGAAAGATAAAGGTAATTGGTTCAGGTAGAACTGATAAAGGGGTACATGCCATCAACCAAACCTTTCATGTTGATATTAATGGTGAAATTAATCTCAATTTGTTAATTAGAAAAATTAACCAGTTGATTAAGCCCCACTGTATAGTTAAAACCTTGGTATTGGTTAACGATAGCTTTCATGCGCGGTTTCAAGTTAAAACTAAGGTGTATGAATATCTGATTAACTGTGGGAATTTAAACCCGTTGCAATTTAACTATGTTTGGCAGTTAAACCAGCAATTGGATCTTGAAAAACTCAAAGCTGATGCCACTTTATTTTTAGGTAAGAAAAACTTTCTTAGCTTCAGTAGTTCGATTCACACTGATTCAATTCGCACAATTAGTAAAATTACCATACAAAAAGAAACTAACCAACTAGTTAGACTAACTTTTTTTGGCAGTGGTTTTCTCAGGAGTCAAGTGAGGATGATAGTTGCTTGTTTAGTGAATTTAAACACTAATAAAATGGCACTTGAAACAGTTGCAAAATTGTTTGAACACCCCAAGAAAGGGAGTTGTGTTGTTAAAGCCCCTAGTTGTGGTTTGTATCTGAAAACAGTGGTATATGAAAAATAGTTACAAGTGGGATCTATCAGTTTTATTAAACAACCAAAGCTTACAAGCAAATTTTTTAAAAATTCAAACAGTTAGTGAAGCGTTAATTAAAGCTTATAACAACGGGTTGTGTTTTACAAATAAAACTAGCTTTGAACAGTTTTTAGCAATCGATGATAAGTTCACTGAACTTGAAAATCGTTACACTAACTACCTTTATAACAAGCAGAATGAAAATAACTTGGATAAGGAGGTTAATGATGCAATTTTTGCATACCAGAGTTTTAAAAATAACCATAACCTTGCTTTCAGTACACTGCAACAGGAGTTATATAACCATGAAAAACTCATTAAGGATTATTTAACTGATCCAAAGCTAGCGGTTTACAAGCGCAACTTAATGTTAGTTTTTCGCGATAAACCCCACCAACTATCTAGTCAAACCCAGAGTTTATTGAGTCAAATTAACCCTTGTTTTAACCAAGCAGAACGGATTTTTAACATCCTTTCAACTGCTGATTTAAACTTGCAACCTGTTGTTTATCAAAACAAAAAATATCCGATTAACAGTGTGAGTGATTATCAGTCCTTACTTGAAAACACTAACAGGGGGATTAGAAAAGCTTGTTATGAGAAGTGGATTGAAATTTATTGAACTAATAGAAACAGCTTAAGTTTAAGTTTGGTGGAAAATTACATCCAACTAGAGAACTTCGCTAAACTAAAGAACCATCCTAGTTACATTGCCCAAACTGCTTTTAATGATGAGATTGAAGTTGGTTTTATTGATTTTGTTTACCAACAAGTAGCTCAGTTTGCCAAAACCTTTCAAGCATTTATTCGCTTAAAAAAACAGATTTATAAACATGTTTTAAAAGTCAATAAAGTTGAACCATATGATCTTACCCTAACACTTTTTAAAACTAAAAAACCATACACGATTGAACAAGCTAAACAAGATGCACTAAAAGTTTTAGATCTACTTGGTGACAACTACATCAAGATAGTTAAAAAGGCTTTTAATGAAAACTGGATTGATTGACTAGCAGATAAAAACAAGTACACAGGGGCATATTCAATCTCCAATGTTAAGGGCTTAGAGCACTTTTTTATCTTAATGAACTTTGATAAAACCAAATCATCACTAAATACGTTGGTACATGAACTTGGTCATTCAGTTCACTCTTGGTATGCATCACAACACCAATCCCAAAACATTGATCCTACTATCTTTTATGCTGAGATTGCTTCTATTGCCAATGAACTGTTGTTGTGTTATTATGAACTGCAACTTTATAAAAATAACCACAAGCAGTTAATTGCTAGTTTATTGAGTCAAATTAACCATTTTTTTGGCGCTACTACAAGACAAATAATGTTTTCACAATTTGAAAAAGATACGCTTGATTTAATCAGAGTTAACCAGAAACCTGACTTTAAAACTTTGATTAAAATTTATGCAAATACTGCGGTTAAATACCAAGGTTTTAAACCTGAAGTAGTTGCTAATAAACTAAAAAAGACCCAGTATCAAAAATCATTGTCACACATCATTGCTATCCCCCATTTTTATGCAGGTAACTTCTATGTTTACAAGTATGCCATTGGCCAAGTTGCAGGTATTTTAGTAGCTAAAAAAATTAATAGTGGTGATAAAAAGATGAAAGATAATTACTTTAAATTCCTCAGTTCAGGTTCTAGTTTAGCGCCACTTGAAACCATTAAACTCTTAGGGATTGACCTTACTTCACCCCAACCCTGACAAGAAGCACATAACGAAGTAAAGCGTTGGCTTAAAATTGTGAAACAAAGCTTTAAAAAACTCCAAAAATAAGTGCACCATTTTAACCGCGCTAAAAAGGCCAAGAATAACGAGTTTTTTACTTTAATTGATGAGATTGAAAACGAAGTAATTAACTACCAAAAGCAGTTTGCAAATAAAACCATTTTTTGTAACTGTAATGATGGTAAAAATTCCCATTTTTTTCAGTTTTTTCAAACTAACTTTAACCAGTTACAACTAAAAAAACTCATTGGGTTTAGTTTTAATAATCTCTCACAAGCTGACAAGTTCACTTTTGATGGAAATAAAGTAACTAAAACCAAATTAAAGGGTAATGGTGATTTTAGTTCTGATGAATCGATTGAAGTGTTAAAACAAGCAGATATAGTTGTAACAAATCCACCCTTTAGTTTGTTTCAAAGTTTCATTGATCTGTTAATACAACACAACAAGCAGTTTCTGGTTTTAGGGTTAAATGCAGCAGTTAGCTATAACCATATTTTTACCTACTTTAAAACTAACAAGTTGTGGTTTGGCTATACCGTTAATAAAACAATGAGTTTTTCAGTTAACAGTGACTATCAACTTTATAACCCCAAAACTAGTAACTTTTTTACAAAAAATGGCAAGTGTTTCCAAAAGATAGCAGGTATCTCTTGGTTTACTAATTTAGGAAAACCACATTATAACCCCTTTTTAAATACCAACTGTTTTTATAAAAACAACGAAAAAAACTATCCCAAGTTTGATTGGTATGATGCTATTTATGTCAACAAGATTAAAAACATCCCTATGGATTGAAATGGATTGATGGGAGTTCCTTTAACCTTTTTAAACTGTTACAACCCCAAGCAGTTTGAATTAGTTGATTGTCTTGCTAACCCTTATGCTACCTTAGATACATTAAAAACAAATGCCTTTGTGAAATTAAATCAGGGTGATGTGAGAAATGTTAATGGTAAAAGAAGGTATGTAAGGGTAATAATTAAAAAACAACAAATTTAGTTTTTTCAACATTTAACAACTCTGTTTTTACAAAAGTTAGCTTAATTTTGCTAAATTGTTTTTTGATAGCACACTGCTGTCAACGAACCATGTTGATAAATGAAATTCAAACTATTTTTTTTATCAAGCAGTGTGTTGGGTCCAATTGCCCTATTTACAACTGCTTGTAGTGCTGTTTATAGGTTTGATCAAGTTGATGATGGCAAGATTAAACTAGCAACTGTAACTTCAGCTTCCGCTAGTGGCTCGCTTACTACTATCATCAGTAAATATAATTCACAAAAAGATCCTAATGATTATCCAGTGGAACTGGTTTCACTTGATAGTAGGGGCAGTTATTCTAATGGCAAAAAGGATCTGCAAGCTAAACTGCTAGCTAAGGATAAAAATAACTTTTATAACCTTACTTTTAACTATAGTGATGTAGTTTCAATCCTCTCAAGAAGCCAGATGGAGTTGAGCTTTGATACGGTTGATACTAGTAATTTTGATCCTAGTTTTCTTAGTTTTAACAATAACATTTCCAATGTTAATCCAAATAGCATCTATGCTTTACCTGCTACTGTATCAGGTGAAGTTTTAGTTTTAAATGGACCGGTGTTACATTACATTTTAAGTAGTGCTAAAAAAGATAGTAACACCACCCTTTCAACCCATTCAGCTAGCAATAATAGCAATAAGGGAACAATGGTTGTTGCTAGTGATAGTGAAACATCAAGTTTATGAACTAAATTAGAAGCTGCAGCAAAAATGAATGCTCAAACTAATGAAACCCAAGTTTTAAAAAGTAATTCATCAGAATCTAACCAAACCCAAGCTAGTGATACAGAGATTAAAAAGATTTGGGGTGATTATCAAGAAGTTGATGGAGGGTTGAAAAATTACACCTTTAAAGCTAGTGTTTTTAACAATTGAAAAGACCTAAATGACTTTGCCACCAGGATTGCAAAATCCTTTACAAAACTGCAAACTACTACTAAAAAAGGGGAGGAAGTACAAGCTGTATTTGGGATTGGTAGTTTGGGAAATGCCTTATATACTGCTTTATTTGCTTCTGGAAAAGCTGATTACAATAACTTTCTTTTTAACATCAAAAACCAGCGAATTAATTTCAGTAACTTTTTTAATAAATCCTCAACTGCATTTCAAAACCTTAAAACTATCTTTAACAGCTTCAAATCCTTAATTGATCAAAACGGTTTAATCTCAAATGCACACTTTAACACCCCAGTTAATGACTATGCTAAGTTTAACCAATTAGCTTTTTACACCTCTTCAACTGCACGCTTTCCCTATTCATTTGCAAGTGATAGTGTAAAGCGTTTAATAGTTAATGACAAGACAATTGAAAACAAAAACAATAAGAGTGTTTTTGAGGTTAATTTAAGTAGTGATAGTGATAACAACAGTAACCTAATTGGTACTGTATCACTGGAAAATAGTAAACAAGTTTCACTCTATGAAAAGCAAGTGGATAGTAATAAACAAATTGGTGTTGATGCTTTGTTAATCAAAGATGAAACTTTAATCAACCATCTTAAGAGTTTAAAATCGCAAGTTAGTGCAAAAAGTGCTAGTGAAACTTCCCAAACAAAACAAAACAAAACCTTTTTAGCATTTACAACTGTGAATGCAGATCAAAAAGCAATCTTTGATGTTGGTAAACTTAATGGCAAAACTGCCAAAATCATTATTAATGCTACTGAAACAACTAATGCTAAGATTAGCACCTTACAAGAAAAAGAGGCAATAGTTCTAAAAGCACCCCAACGCTTTGAGAGCACTGATCCATTTCCTATTGCTTTAGTGCAAGGTCCTAGTTTAATAGGGATCCATGCAAATGAAAGAGAGGATATTGAAACCAAAAAGTTTGTGAATTGGTATCTGAATACTAAAGTCCAATGGGAAGAAAATTCTATTAAAACTCCTGCAGAATATGTAGCTGATAAAGCTTCTTATCTTTTACCTTTTAAAAATAGGCTAAATAATACTAACAGTTACAATGAGTTTGTTAAAACTGCAGTTAGTCAGTTTGCTGATAAAAATGTAACTAAATTTGCAGAACCTGCTGATTTTTTATCCAACAAAGTACGTGATGGTGTTAAGAGTAATTTAAATGCTGCAATTAACAACCACAGCATTGATTTTGATAGTTTTATCAATGATTTAACAGACTATCTTGGTAGTGATGTTAAAAACATCTAATAATTTATTCCCCCAACCTCTATTTTGTGTTACTAGTGCCTAAGGTGGTAGTGGAGTACCACCAACTTGATAAGAAGGTTGTCAAAGAGAGTTTGGAAGTGGAATCTTCTTCCTCCTTCAACCCCACCCAAGGGTTGAAGAAAGATAGTCCAATGAAGGATTCAAGTAAACAAGGGGAGAAACTCAGTGAAGCAACTTCATCGATGAGTAGTGGTGGGGCTACCTCTCCTCGCAAGGCCCTTAAGATAGAGGTGGAGAAAGGCAGTAAAGTCAACCAAGGCGAACTACAATCCAACGACTTTGCTAAAAAGCCGTTGCAGCATAAGAACAATAGTGGGACAGATGTGAAGTTGGATGCGAGTGGGGAGTTTGCTGACGACAAAGCCTGAAAACCGGTGTTGACTACCGAGCAAATACAAGAAAAGAGGGGGATGGGGGCTTTCTCCAACTCCCCCTCCCCTTCAACTTCTTCTTCTTCTTCCCCACTCCCCACTTTTTCTAACATCAATGTTGGGGTTAAATCAATGATCACTCAACATTTAAATAAAGAAAACACCCGGTGGGTGTTTACCCCTAACTCTTCACCAGACATTTGAACGGGTGCAGGTTATAGAAAAGCTAATGACAACAATAACGGTATCTCCTTTTGAACAGGTGAAACCTAGCAATGGTAGCAACACGTTTAATCCCAATTCAGATGATAATAAAGTCACACCATCAGGTGGCTCCCCAGCCAAAAAAACCACCTATGATTTTTTACCCAACTCCATCAGTCCCACCAGTGACTGGATCAATGCATTGACTTTCACTAATAAGAACAACCCGCAACGAAATCAGTTGTTGCTCAGAAGCTTACTAGGAACGATCCCGGTATTGATCAATAAGAGTGGGGATAGTGGGGATGAGTTTAACCATACGAGTGACCAGAAATGGGATAAAACGAATGAAAAAGATGGGAATTTACCTGGGTTTGGGGAGGTGAATGGTGGTTTTATTATGTTTTTATCAAATCTTTATTTACTTTAATAGTTAAAAAAGTTTTGAATTTTTCTTAGTTTTTTATTTGTTTAATATTTAAGAAAGTCTCAAATTTTTATCAGTTTATTGGTCAAAGAAGTCGCAAATTTTCTTAATTTATTTATTTAATAGTTAAAAAAGCGTTAGGTTTTTCTTAGTTTTTTATTTGTTTAATATTTAAAAATTTCTTGATTTTTATCCTTTAGTTAATTAATAAAACCTTTACCCCTATTATCACAAACCATCCATAACACCAACTTGTTTGTGTTGTTCAAGTCTAGGGATGTGAAAGTTAAGTATGAGTCAAGTGGCTCAAATACCAACACAATTAGTTTTGATTCAACTAGTCAAGCTAACAAACCATCCTATGTGGTCGAGTTTACTAACTCCACCAACATTGGCATCAAGTGAAGCGTGGTGAAAAAATATAAGTTAGATCTACCAAATGTTACCAATGAGATGAACCAAGTGTTGCAAGAATTGATCCTAGAACAACCTTTGACTAAGTATACCTTAAATTCGAGTTTGGCTAAACAAAAGGGCAAAAGCCAGATAGTGGTGCATCTTGGAAAATCAAATAAGTGACAATCCATGCGCAACCAACACAACCTAAACAACAATCCCTCTCCCAATGCTTCAACCGGATTTAAATTAGATAAAGGCAACGCATATAGAAAACTAAGTGAGTCCTGACCAATTTACCAACCAATTGATGGGACCAAGCACGGCAAAGGGAAGGATAGTAGTGGGTGGAATAGTGAAGAAAAACGACTAAAAGTGATGCGCCCAGTGTTTCTGGAGGGGGAACATCAGATCAAAGTAATAAATTCACCAAGTACCTCAACACCAAACAAGCGTTAGAAAGCATCGGTATCTTGTTTGATGGGGATGGAATGAGGAATGTGGTTACCCTCCTTCCTCTACTTTCAACCCAACAAGGTGAAAACAAATGCTTACCAAACCACTAACACCTACAACAAGTTAATTGAACCGGAAAGTGCAACAAGTGCAGCAACTAACATGACCAACTTGTTAAAGCTGTTGTCTAGTAAAAACATCAAACAAAAGTTGGGGAAGGGAACAGCTTCTTCGCAGGGAAGTTTCAGTGTCCAAGACACCTTTTCGTTTGTTGTTCCTTATTCATCGAATCACACAAATAATGGAAACACTTCAGGAACCATTAAAACTGCTTATCCGGTGAAAAACACAGAGAAAGCAACAGTAATGATCAATTCCTTGATCAACGCTACGCCGTTGAATAGTTATGGGGATTTAAATAATCACACCAAGTTAATTTACCCAAATTATTTACTTATTTATTAACCATTGTTACCCAATTTTTCAACCACTTCCCCACTCTATAAATTGTTATTTTTTAACAATTTGAACAATTAAATTGCAAAAAATAACTATATTAATGTAAATTTAGCTAACAAGCTTAACTGGTTGTTTTTGAGATGAAAGGGTTTTTAAAACCAAATTTCTCTCTCGGTGCTTTGTTTTTAACTTTAAGCCCCATAGCCACTGCATGCATTGCTGAAAAACCAGTTAACAACCGCTTTAACTTTAATAGCGAGCAATTAGCTAGGCTAAGAAAAGCAAGGGTTAACCACTGAAGAGATGGGGATACTTTGGAAGTTAGCTTTGCAAATAACCACCAAAAACCGATCCGTATCTATGCCATTGATACCCCTGAAAAAGCAGTTTTATCTATACAACGCAAATCAGAGATAGAACTTAAAGAAGCTAATAAAGCAACTGAGTTTGCCAAAAGCTTAATTCCCATTGGTAGTGAGGTGTGGATCTGACCACTAAATAGCTATAGCTATGATCGTGAAGTAGCTGCAGTGTTTTTCAAAACCAATCCATTGCAACTGCACTTTGAATCGTTTGCAGTTGAAATGGTAGCAAATGGTCATGCTTTACCTATTGCTGGTAATGACTTTGATCTTGTTTTTAGTGATTTGGACCCATTTAATCCCCTAAAAATAGTAGGGATTGAACTAGCTAATGGTTTAAACAATGCTTTTAACAACAGAAAAAACATCTTTAGTTATTTAGAAAACAGCTTTCAATCAATAACAATGGTCTATCAACAACGCGGTGTTGACCAAGGTTGAACAAGGTATTTAGCTCCTAGTAATGATTTTTCCTCTACTAAACTGGGGTTGGGATTAACCATCTATGAATTGAAACTAAACAATGGCTAACAATAAGAGTGCAATTGAGTTGAAAAACATCGTTATTGATTTTGGTGAATCAGTTGCGATTGACAACATTAACCTTAGTGTTGAAAAACACCAACTAGTTAGCTTACTTGGTCCTAGTGGTTGTGGTAAAACCACTACACTTGCAGTTATTGCAGGACTTATTAAACCAACTAGTGGTCAGGTGTTATTTAATGGTTATGATGTCACCAAAAAACCACCCCAAGAACGTAAACTAGGGCTAGTTTTTCAAAACTATGCACTTTATCCGCACATGAATGTGTTTGAAAACATTGTTTTCCCCCTCTACAGTGATAACTCGTGAAAACAAGCAGTTTTGGAAAAAAACAGTGTTGCAAACCATGAGATTAACTGTTTGTTACTTACTAGCAACGGTGCATCAGTTCAAGAGATTGATCAGCTCAATAAGTTATTTCATGATAGTATTGAAAAACCCAAACAGATCCAATACCAAATTAATGACCTTAATGTTAGTGTTTTTAAAAACTTAAATGAACTAACTGCAAACCTTAAGTTAATACCAAGTAAACACCAGTTTGCTATTACCAATCTCAACAAACAAACTCTAAAACAGATTAATGAACTGGAAGCTGAGTTTAAAACAAAGTGAAAGTTACAAAAACAAACCCCAATTAAGAGTGGGGTTGAACACAATGCCAAACTCCAAGCAATTAAACAACACTTTAGTTATGAAAAACAACGGTTAAAAAAACACTATTTCAAAACTAAAGTGGAACTAAAACAAACCCTTGTTGAAAACCTCAAGTTAGTTAAAAAAGCGATTAGTGAACAAACTAAGTCAATTAAACAGAGCAGTGATTACACTAAGTTAAAGCAACTAAAACGGTTAATTAAAGTTGAACCTAACCAACTCAAAAAACAATATAAGGTTTTTCTCAATCAGTTAATTAAAAACTATTCACTTAAAACTGATAAGTTAACTGATACTCAACTTAATGAAATTGAACAGATTAAAACCAGAATTGTTTCAATAAAACAGTTTATCAACAAAACTGCACTTGAAGTTGCTAACAAACTAGCGATTACCAAGATTTTAACCAAACGCCCTGATAAGATTTCTGGTGGACAACAACAACGCGTAGCAATTGCTAGAGCAATTGTCAGAAGACCTAAACTATTGTTAATGGATGAACCACTCTCTAACTTAGATGCAAAGCTAAGGGTACAGACAAGACAGTGGATCAGACAGTTTCAACAGGAGTTACAAATTACCACTGTTTTTGTCACCCATGACCAGGAAGAAGCGATGAGTATTAGTGATGTCATTGTTTGTATGTCAACTGGAAAAGTGCAGCAAATCGGCACTCCCAGTGAACTTTATTTAAAACCTGCTAATGAGTTTGTTGCGCGCTTTTTAGGCACCCCTGAGATGAACATCATTGAATGTAGTGTCAAAAACAACCAGTTGTTTTGAAACAACCATCTGTTAGTTACTGAGAGTTTTAAGCTTAATGTAGAGAAACTCTTAGTTGGGTTTAGGTATGAACAACTAGTGGTCACTACTAACAAAAGTAGTTTGCAAGCTAAACTAATTAACATTGAAAACTTAGGTAAACACTTAGTTGCTACCATTAGTTTGTTTGATACCACCTTATCAATGCGCTTAGAATTGAATAGCCACTTAAAAGTAGGTGATAGTTTAAATTTCATTATTAAAGCTAACAACCTCCATTTTTTTGATATTGATACAAAACAACGGATTGAGATTTAACAATGTTTAAGTGGTTATTAAAACATCATAATCAACCTCATAGCCTCCAGTTAGGGTTACTAGACCAACCATTACCGTTCTGAAAGCCCTTTTTGTTGTTCCTCCCTGCGCTTTTAACAACAATTTTGTTTACCATTATCCCCTTCTTTTTAAGCTTACAGAAGGGTTTTAGTGCTAACAGTGATCTGTATGATCTCTCCTCCCAATCCTTTAGTTTACGAACCTTTCAGGATCTGTTTAGTGAATCTAACTTTGTGTTGGGCTTACGTAACAGTTTTCTCTATTCACTAATCTCTTTACCCTTTAGCATTATTATTGCTATTGTCATTGCCAGTGCCATAGTATTTGTGTACAAAAAGCTGTTAAGAGGGTTTTGACAGACCGTGTTTTTTTTACCCTATGTAACTTCAGGGGTGGCAATCTCGATTGCCTTTGTATATATCTTTGATTCTGCCTCTGGTATTTTAAACACGGTGTTTAATGTCAACACCAAGTGGCTTGATTCAGGTTCACGTGATACATTTAATGCCTTGTGGGCTATCTTAATCTTTGGGGTGTGGAAAAACTTGGCATTTAATGTGTTGATTATCTCAACAGCAATGTTAAGTGTTAATCCCCAACTTTACAAGGTAGCAAGTTTAGATAGTGCCAATCCTGTAAGACAGTTCTTTAAGATCACTCTTCCCTCCATCCGTCCTACTTTAATCTTTCTTACTACCCTTTTAATCTTAGGGGGGATGCAAGTCTTTCCGCTGGCTTTGTTTGAAAACAAACCTGAAGAAGCGGTTGCTAATGGGGGGAATAGTATCTTGCTTTACATCTTTCAACAGATCCAAAGTGGCAACACTAACTTAGCAGGTGCTGCTACTTTAGTGTTGCTTGTGTTGGGGGTTTGTTATGGGTTAGTGTTACGCAATGGTTTTTATCTGATTGAATGGTTGCAGTGAAAAATTAAACAGCTTTATGTTCAAAAACAACTTACGCTTTACTAGTTGAATTAATCAACACAAGTTTTACCAACTTGATCTGAGCTTAAAAACCCGCTCAATCAAACAAATTGTTTTAACGTTGGTTTTCAAAACATTGGTATTGGGGTTTTTTGGGTTAATTGTCATCTTTCCCTTTTATCTGATGGTTGTGGTTAGTTTTGCTAGTGATGAAAGAGCATTAGACACAAGAACCCCAATCCTTTGACCTGATAGTTGGAACTTTGATAACTTTAGTAGGGTGTTAAGTGATGGCAAGTATCTCAATGCAATAGTTGTCAATACTTTAGTAACGGTACTTTCAGTGTTACTAACATTGTTTTTTACCATTTGCATGGGTTATAGTTTTTCACTACGGAAGTGAAAATACAAAAAACTGGTGTGGTTTTTCTTTCTTAGTGTGTTAATACTGCCTGAGTCTGCGCTTTTAATTGGTCAGTATCGGATTGTAATAGTTGCTAACTGAAATAACCCCAACAGTCCCTTGATTGTTCTGGGACTCATTATGCCCTTTGTTAGCAGTGTTTTTAGTGGGTTTATGTACCGTACTAGCTTTGAAGCCATTCCATCTCAATTAAAAGAGTCAGCACTCATTGATGGGTGTAATGGCTTTAACTACTTTTTGAAGATTGCTTTACCAATGGTGAAATCTACCAGTTGAACAGTGGGGATTTTAACTGCATTTAGTGCTTGAAATTCCTATTTATGACCATTACTGTTGTTGGGCAACAGGGTGGATTTAAACATTAACTTGTGGGTGTTACAACAAGGGATCTTGGATGCTAACAGTAGTGATGAACAGATCAGAACGCTGTTAAATCTCAAGATGAGTGCAGCGATTCTAGCCATCCTTCCGATGTTTATTATCTACTTTTTGTTCCATAAAAGGATTATGAATGCCATTAAAAACAGAGCCAACACCATTAAGGGTTAATATGCAAAAGTTTAAACAACTGGTTGGTGCAATGCACAGATGGGTAAAACTAGCACTATTAGTAATCATTGTGTTATTGGGGATTATCTTTTGTCTGTTTGCCATCTATGGCATTGCGCAAGTGATCATTACCATTATCAATGAAGGGGCACTTTTATAATCTTTGTTATGAAAAAAGGATCAATAACTGAAGCAATTAATGCCATTAAACAATTTGATAAGATTGTTATCTTTCACCATGTGCGCCCTGACGGGGATTGTTTAGGAGCACAACAAGGTTTGTTTCACCTCATTAAAGCTAACTTTAAAGATAAGGAGGTGAAGTGTGTTGGTAATAACAACAACCTGTTTAGCTTTATCAACATGACATTTACCAACCAAATTGATGAGAGCTTTTTAAAAGAAGCACTTGCGATTGTGGTGGATGCTAATTACAAAAACAGGATTGAATTGAGAGAACTGTTAGATAAAAACCTGTTTAAAGCAGTGTTAAGGATTGATCACCATCCCAATGAAGATGATCTAAACACTAGCTTTAACTTTGTTGAAGAAAGCTATGTAGCTTGTTGTGAGCAGATAGTGGAGATGGCCACAGTGGCGAAGTGGACCATACCACCAGTGGCTGCTACTTTACTATATATAGGTATCTATACGGATAGTAATAGATTTCTATATAGTAATACATCATATAGAACACTATACTTAGCAGCAATACTATATAAAGCTAAAGCTGATATAAGGATAGTACATGATCATTTAAACCATACTAGTTTAGCAGATCTTAAGTTTAAAAAGTATGTTTATAACCACTTTAAAACCCAAGGACAAGTGATCTATTTTATCTGTACTAAAAAGATCCAAAAGAGACTAAGAATGACTGCAGATCAATGTGCTAGAGTTAACTTGTTAAGTAACATAGCAGATTACAAGATCTGACTTTTCTTTATTGAACAAGCTAATAATGAGATCAGGATAGAACTGAGGAGTAATGGGATTAATGTCAGAGATATAGCCATTAAGTATGGTGGGGGAGGACATAATAATGCAAGTGGAGCGATCATTACTAACAAAAAACAAATTAGTGATGTTGTTAGTGATTGTGTGAAAAAAATTGTTTATAATTAAGTTTGTATGCACCAACCAAAGAAAAGACTGGCTAAGAAGTCTTGAGCCTTTCTAACCGCTGCACTTACCCTTGGGGTTATAACAGGTGTAGGTGGTTATTTTCTCTTTAACCAAAATAAGCGACGTAGTAGCGTGAGCAACTTTGCTTACCAACCCAAGCAGTTAAGTGTTAAACACCAACAAGCAGTTGATGAAACCTTAACCCCTTGGACTTGAAACAATAACAACTTCTCTTCACTGAAGATTACTGGAGAGAACCCAGGATCATTTGGACTAGTAAGAAGCCAAAATGAGAACTTAAACATCGCAAGTGTTACAAAGAATGGTAGTGATGATAATCTCAAGTATCTTAATGCTGTTGAGAAGTACCTTGATGGTCAGCAAAACTTTGCAATCAGAAGGTATGATAACAACGGTAGAGCTTTATATGATATTAACTTAGCAAAAATGGAAAACCCCTCAACGGTGCAAAGGGGTTTAAATGGCGAGCCTATCTTTGATCCTTTTAAAGGCTTTGGTTTAACTGGTAATGCCCCTACTGATTGGAATGAGATCAAAGGTAAAGTTCCAGTAGAAGTAGTTCAATCCCCCCATTCCCCCAACCTCTATTTTGTGTTACTAGTGCCTAAGGTGGTAGTGGAGTACCACCAACTTGATAAGGTTGTCAAAGAGAGTTTGGAAGTGGAAGCAACTTCCAACTTTGATCCAACTAAAAGGTTGCAAAAAGAGAGTCCACAAAAAGATTCAAGTAAACAAGCAGAGAAACTCACCGAAACAACTTCATCGATGAGTAGTGGTGGGGCTACATCCACTCGAGCCAAGGCCCTCAAGGTTGAGGTGGAAAGGGGGAGTCAAAGTGATTCACTTTTAAAAAACGACTTTGCTAAAAAGCCACTGAAACATAAAGAACAATAGTGGGGTTGGATGCGAGTGGGGAGTTTGCAGGAGACAAGGCCTGAAAACCATTGTTGAAAACAGATGAGATAGCGAAGGAGAAGGGGATGGGGGCGACGGTGGTTAGTTTCTATGATGCACCCTACAGTGAAAACCATACTGCCTTTGGACTTGTTGATCACATCGATCCTAAAAAGATGGTTGAAAACTACCCACCAAGTTGAAAGACCCCGAAGTGAAACCACCATGGGATCTGGGATTACAACGCAAGAAACCTCTTGTTACAAACAACAGGGTTCTTTAACCCAAGAAGACACCCAGAGTGGTTTGATGAAGGACAAGCTAAGGCAGATAACACTAGCCCTGGCTTTAAGGTAGGGGAAAGTGATCACAAAAAAGACGGCTTTAAAGACTCTTCTTCTTCTTCTTCTCCAATAGCTTTACCATTTGAAGCATACTTTGCTAATATTGGTAACATGGTTGCTATTGGCAACTCGGTATTTATCTTTGGTGGTAATGGTCATGCTACTAAGATGTTTACCACCAATCCCTTAAGTATTGGGGTATTTAGGATTAAATACACTGATAACTTTAGTAAGTCATCAGTAACTGGTTGACCATATGCAGTGTTATTTGGAGGATTAATTAATCCCCAAACCAATGGCTTGAAGGATCTTCCCCTTGGTACCAACAGGTGGTTTGAATATGTACCAAGAATGGCAGTTAGTGGGGTAAAATGGGTTGGTAATCAACTAGTGTTAGCAGGAACACTAACAATGGGTGATACAGCTACTGTACCTAGGTTAAAGTATGACCAACTAGAAAAACACCTAAACCTAGTTGCTCAAGGCCAGGGACTATTGAGAGAAGACTTGCAGATCTTCACTCCCTATGGGTGAGCTAATCGCCCTGATATTCCTGTAGGAGCATGACTCCAAGATGAAATGGGCAGTAAATTTGGTCCCCATTACTTCTTAAATAACCCTGATATCCAGGACAATGTTAATAATTATACGGTTGAAGTATTAATCAATACCTATACAAACACTGATAAGTTAAAACACGTTTATCCTTATCGATACAGTGGTTTGTATGCTTGACAGTTATTTAACTGGTCTAACAAACTAACCAATACTTCCCTTTCCGCTAACTTTGTTAATGAAAACAGTTATGCACCAAACAGTTTGTTTGCTGCTATCTTAAATGAAGATCTGTTAACAGGGCTAAGTGATAAGATTGTCTATGGTAAGGAGAATGAGTTTGCTGAAAATGAAGCAGATAGGTTTAACCAACTTTTAAGTTTAAATCCTAGTCCTAACACTAACTGAGCTAGGTATTTAAACGTAGTACAACGTTTTACTACCGGACCTAACCTTGATAGTTCTACCTTCGATCAGTTCTTAGATTTTCTCCCCTGAATCGGCAATGGTAAACCCTTCTCCAACTCCCACACTGCTACCCTTTCTGTTAGTTCAAATACCCCCCTCCCCACTTTTTCTAACATCAATGTTGGGGTTAAATCTGATATCACTCAACATTTAAATAAAGAAAACACGCGGTGGGTGTTTACCCCTAACTCTTCACCAGACATTTGAACGGGAGCAGGGTATCGCAAACAAAGTGCTAACCAAAATAACGGCATTCCTTTTGAACAGGTGAAGCCTAGTAATAGTAGTACCCCCTTTGATCCCAATTCTTATGAAAACCAAGTCACACCATCAGGTAGCTCCCCCAAAACAACCACCTATCCTTCTTTACCAAACTCCATCAGTCCCACCAGTGACTGGATCAACGCATTAACCTTCACTAATAAGAACAATCCACAACGCAATCAGTTGTTGTTAAGGGGGTTGTTAGGAACTATTCCGGTATTGATCAATAAGAGTGGGGATAGTAATGATCAATTTAACAAGGATAGTGAGCAGAAGTGGGATAAAACTGAGACAAATGAGGGCAACCTCCCAGGGTTTGGGGAGGTGAATGGGTTGTATAATGCCGCATTACTCCATACCTATGGTTTTTTTGGCACCAATACCAACTCTACTGATCCTAAGATAGGCTTTAAAGCTGATAGTAGTAGTAGTAGTAGTACACTAGTAGGTAGTGGGTTAAACTGAACTAGTCAGGATGTAGGTAATCTTGTTGTAATCAATGACACCAGCTTTGGGTTTCAACTTGGTGGTTGGTTTATTACCTTCACTGACTTTATCAGACCAAGAACTGGTTATCTAGGAATTACCTTAAGTAGCTTACAAGATCAAAACATTATCTGAGCAGATCAGCCTTGAACTAGTTTCAAAGGGAGTTATCTAGACAGTGATGGTACCCCTAAATCACTGTGAGATCCAACTGCTTTAAAATCCCTTCCAAATAGTTCAACTACCTCTGATACCAATCCTACCCTCTCACCCTCCTTCCAGCTCTACCAACCCAACAAGGTGAAAAGTGGTCAATACCAAACCACCAACACCTACAACAAGTTGATTGAGCCTGACAAGTGAGAATCATCAAGTGATTTGACTAACATGACCAACTTGTTAAAGCTGTTGTCTAGTAAAAACATCAAAGAAAAGTTGGGGAAGGACACCCAATCAATGGGAAAATAATGGAGGGGGTGTTAGTCAAACGATTAACACCATTACCACTACGGGAAATATTAGTGAAGATCTAAAAGAAGAAACTAGTATTCAAGCAGAAACACTTAAAAAGTTCTTTGATAGTAAACAAAACAATAAGAGTGAAATAGGGATAGGTGATAGTACATTTACCAAGATGGATGGTAAACTAACTGGCGTAGTATCTACTCCTCTTGTTAACCTTATCAATGGTCAGGGAGCAACTAGTGATAGTGATACTGAAAAAATTAGCTTTAAATCTGGTAACCAGATTGACTTTAATAGGTTATTTACCTTACCAGTAACTGAACTATTTGATCCTAACACGATGTTTGTCTATAACCAGTATGTACCACTATTGGTTAACTTACCTAGTGACTTTGATCAAGCTTCAATCCGCTTAAAGGTAATTAGTTACTCAGTAGAAAACCAAACCTTAGGAGTTAGATTAGAGTTCAAAGATCCTCAAACCCAACAGTTTATCCCGGTACTAAATGCATCAAGTACAGGTCCCCAAACTGTCTTTCAACCCTTTAACCAGTGGGCAGACTATGTCTTACCTTTGATTGTAACTGTTCCTATAGTTGTCATTATCCTTAGTGTTACTTTGGGATTAACGATTGGAATTCCAATGCACAGAAACAAAAAGGCATTACAAGCAGGGTTTGATCTTTCTAACAAAAAGGTTGATGTTTTGACCAAAGCAGTTGGTAGTGTCTTTAAAGAGATCATTAACAGAACAGGGATCTCTAACGCTCCTAAGAAGTTAAAACAAGCTACCCCAACCAAACCAACTCCTAAAACCCCACCAAAACCTCCAGTAAAACAATAAGATGAAAACAATGAGAAAACAGATTTATAAAAAAGCATACTGGTTACTATTACCCTTTCTACCATTAGCACTAGCCAATACCTTCCTTGTCAAAGAGGATAGTAAGAATGTTACTGCTTACACTCCCTTCGCCACCCCTATCACCGATTCTAAAAGTGATCTGGTTAGTTTGGCACAACTTGATTCTTCCTATCAAATCTCCGACCAAACCATCCATAACACCAACTTGTTTGTGTTGTTCAAGTCTACTCAGGTAAAAGTTAAGTATGAGTCAAGTGGCAGTAACAACCAGATTAGTTTTGATTCAACTAACAACAAACCATCCTACATCGTTGAATTTACTAATTCTACCAACATTGGCATCAAGTGAAGCGTGGTGAAAAAGTATCAGTTAGATGTACCGAATGTTTCTAGCAACATGAACCAAGTGTTGCAAAACCTCATCCTTGAACAACCTTTGACTAAGTATACCTTAAACAGTAGTTTGGCCAAACAAAAGGGCAAAAGCCAGATAGAGGTGCATCTGGGTAGTGGGCAAGCAAATCAGTGAAACACTCAACGCAACCAACACAACTTAAACAACAATCCCTCTCCCAATGCATCAACTGGATTTAAATTCACTAAAGGCAATGCGTATAGAAAACTAAGTGAATCCTGACCAATTTACCAACCAATTGATGGGACCAAGCAGGGCAAAGGGAAGGATAGTAATGGGTGGAATAGTGAAGAAAATACGGCAGCAGGGGATGCGACCAGTGTTTCTGGAGGGGGAACATCTTCTGGAACATTTAATAAATACCTCAACACCAAACAAGCATTGGAAAGGATCGGCATCTTGTTTGATGATCAAACCCCAAGAAATGTTATCACGCAGCTCTATTATGCTTCAACTAGTAAGCTAGCAGTCACTAACAACCACATTGTCGTGATGGGTAACAGCTTTCTACCCAGCATGTGGTACTGGGTGGTGGATAGGAGTGCAACAACTGATTCATCATCAAAACCCACCTGGTTTGCTAACACCAATTTAGATTGGGGAGAAGACAAACAAAAACAATTTGTAGAGAATCAGTTGGGGTATAAGGAAACTACCAGTACCAATTCCCACAACTTCCATTCCAAATCTTTCACCCAACCTGCATATCTGATCAGTGGCATTGACAGTGTCAATGATCAAATCATCTTCAGTGGTTTTAAAGCGGGCAGTGTGGGGTATGATAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTACCAAAGACCAAGCACTTGCTTGATCAACCACTGTTAGCTTAGATAGTAAAACGGGGTATAGGGATCTAGTGACCAACGACACGGGATTAAATGGTCCGATCAATGGGAGTTTTTCAATCCAAGACACCTTCAGCTTTGTTGTTCCTTATTCAATGAATCATACAAATACGGGAACTAGTGGAACCATTAAAACGGCTTATCCAGTGAAAAAAGATGAAGCTTCAACAGTAATGATCAATTCTTTGATCAACGCTACGCCGTTGAATAGTTATGGGGATGAAGGGGTTGGGGTGTTTGATGCGTTAGGTTTAAACTATAACTTTAAATCTAACCAAGAACGTTTACCTTCCAGAACTGATCAGATCTTTGTTTATGGGATTGTCTCCCCTAATGAATTGCGAAGTGCTAAAAGTTCTGCTGATTCAACTGGTAGTGATACAAAGGTAAACTGATCAAACACCCAATCACGTTACCTCCCTGTTCCCTATAACTATTCAGAAGGGATCATTGATGCAGATGGATTTAAGCGTCCTGAAAACAGGGGTGCTAGTGTAACTACCTTCTCAGGGCTTAAATCAATTGCCCCTGATGGTTTTGCTAACTCAATAGCTAACTTCTCAGTTGGGTTAAAAGCAGGAATTGATCCTAACCCAGTGATGAGTGGTAAGAAAGCTAACTATGGAGCGGTTGTGTTAACACGGGGGGGTGTTGTTAGATTAAACTTTAACCCTGGTAATGATTCATTGCTTTCAACAACTGATAACAATATAGCACCTATCTCCTTCTCATTTACTCCGTTCACAGCTGCTGAGAGTGCAGTGGATCTCACTACCTTCAAAGAAGTTACCTATAACCAAGAATCAGGATTGTGGAGTTATGTCTTTGACAACTCCTTAAAACCAACCCATGATGGTAAACAAATTGCTGTCACTGATAACAAGGGCTTTAGTGTTATCACTGTCTCAAGAACTGGCATTGAACTAAACCAAGACCAAGCTACTACAACTCTTGATGTAGCACCTAGTGCACTAGCAGTGCAATCAGGGATCCAATCTACCATCCAAACCCTAACTGGAGTACTCCCACTTAGTGAGGAATTCAGTGCAGTTATTGCTAAAGATAGTGGTCAAAATAAGATTGATATCTATAAAAACAACAACGGGTTGTTTGAAATTGATACCCAACTAAGTAATAGTGTTGCCACCAACAACGGTGGGTTAGCACCTAGTTACACAGAAAACACGGTTGATGCATGGGGTAAAGTTGAGTTTGCTGATAACAGTGTATTGCAAGCAAGAAACCTAGTTGATAAAACTGTTGATGAGATCATCAATACCCCTGAAATCTTAAATTCCTTCTTTAGATTCACCCCTGCTTTTGAAGATCAAAAAGCTACCCTTGTTGCTACTAAGCAAAGTGATACGTCACTTAGTGTCTCACCAAGAATCCAGTTCTTAGATGGTAATTTCTATGATCTTAACTCCACCATCGCTGGGGTACCTTTAAACATTGGTTTCCCTTCAAGAGTGTTTGCTGGGTTTGCAGCACTCCCTGCATGGGTGATCCCTGTATCAGTAGGTTCTTCAGTTGGGATCTTGTTTATCTTGTTAGTCTTAGGACTTGGGATTGGGATCCCAATGTACAGGGTAAGAAAACTCCAAGATGCATCGTTTGTTAATGTCTTTAAAAAGGTTGATACACTCACAACTGCTGTCGGTAGTGTGTACAAAAAGATTATTACCCAAACTGGTGTGGTGAAAAAAGCACCTAGTGCATTGAAAGCTGCTAATCCTAGTGTTAAAAAACCTGCTGCTTTTTTAAAACCACCTGTTCAACCACCAAGTAAACCTGAAGGGGAACAAAAAGCTGTTGAAGTTAAGTCAGAAGAAACCAAAAGTTAGTTTTTAACCTTTCAATAACCTAAAACACAATCTTTAAAACAAGGTTGTGTTTTTTTGTTTTTTGTCACTTTTCACTAAACTTGCAATTTAGAGAGTGGATATGAAAAGAACAGTTAAAAAAATAAAACCTGACCACTTGTTTAATAAAAAGCAGTGGCACTTACTGAGTGAAGAGATCAGTGATAACCCAATGATTAAGCGTTATTGACTCAAACAACAAAAGCGTCAGCTTGCCAAACTAAAAAAACGCCGTTATTTTAAAAATTAACTATGTATTCTACTTTAAGACAATATAAACCATTGAAAAGAAAGAAATGGTTAAACAAGATCTCAAAACGGAAGTTAAAACAACTTTATAACAAAGCTTATTAGTTTTGTTTTTTATTTTTACTAGTGCCTAAGGTGGTATTAGAGTACCACAAGCTCAGTAAGGATGTAGTCAAAGAGAGTTTGGAAGTGGAATCTTCTTCCTCCTTCAACCCCACCCAAAGGTTGAAAAAAGATAGTCCAATGAAGGATTCAAGTAAACAAGGGGAGAAACTCAGTGAAGCAACTTCATCGATGAGTAGTAGGGCTACCTCTCCTCGCAAGGCCCTCAAGGTTGAGGTGGAGAAAGGGAAGTTGGATGCGAGTGGGGAGTTTGCCAATGATAAAGCCTGAAAACCGGTGTTGACTACCGAACAAATAAAAGATAATAGGGGGATGGGGGCGACTTAGACTTTCTCCCCTGAATCGGCAATGGTAAACCCTTCTCCAACTCCCACACTGCTACCCTTTCTGTTAGTTCAAATACCCCCCTCCCCACTTTTTCTAACATCAATGTTGGGGTTAAATCTAATGATCACTCAACATTTAAATAAAGAGAACACCCGGTGGGTGTTTACCCCTAACTCTTCACCAGACATTTGAACGGGAGCAGGGTATCGCAAACAAGGTAACAATAACGGCATTCCTTTTGAACAGGTGAAACCTAGTAATGGTAGCAACACGTTTAATCCCAATTCTTCTGAAAACCAAGTCACATCAGGTAGCTCCCCCAAAAAAACTACATATGATTTTTTACCCAACAGTATCAGTCCCACCAGTGACTGGAGCAATGCATTGACTTTCACTAATTAAGAATAACCCGCAACGAAATCAGTTGTTGCTCAGAAGCTTACTAGGAACGATCCCGGTATTGATCAATAAGAGTGGAACGGGAGATGAGTTTACCCATACGAGTGAGCAGCAGTGAAACGAAACAGATAAATTAGGAGGTAACCTCCCGGGGTTTGGGGAGGTGAATGGTGGTTTTTATCAACTAAATAAAAACTTATTAGCTTATTTTTATTAGGTTTTTATTTATTCAATAGTTAAAAAAGCGTTAAGTTTTTCTTAGTTTTTTATTTGTTTAATAGTTAAAAAACACTAGGCTTTACCTTTATTTAATTAATAAAACCTTTACCCCTATTACCAAACCATCCATAACACCAACTTGTTTGTGTTGTTCAAGTCCAAGGATGTGAAGCTTACATATAGTTCAAGTGGCAGTAACAACATTAGTTTTGATTCAACTAACAACAAACCATCCTACATCGTTGAATTTACTAATGCTACCAACATTGGCATCAAGTGAAGCGTGGTGAAAAAGTTTAAGTTAGATGTGCCAAGTGTTTCAACAACCATGAACGAAGTGTTGCAAAACCTCATCCTTGAACAACCTTTGACTAAGTATACGCTTAATAGTAGTTTGGCTAAACAAAAGGGCAAAAGCCAGATAGAGGTGCATCTGGGTAGTGGGCAAGCAAATAATTGACAATCGATGCGTAACTCCAACAGTCTAAACAACAATCCCTCTCCCAATGCATCAACCGGATTTAAATTAGACAAAGGCAACGCATATAGAAAACTAAGTGAATCCTGACCAATTTACCAACCAATTGATGGGACCAAGCAGGGCAAAGGGAAGGATAGTAGTGGGTGGAATAGTGAAGAAAATACGGCAGCAGGGGATGCGCCCATTGTTACAGGTGGGGGAACATCTTCTGGAACATTTAATAAATACCTCAACACCAAACAAGCATTGGAAAGGATCGGTATCTTGTTTGATGGGGATGGAATGAGGAATGTGGTTACCCTCCTTCCTCTACTTCCAACCCAACAAGGTGAAAAGAGATCCTTACCAAACCACTAACACCTACAACAAGTTAATTGAACCTGACAAGTGACAATCAAATAGTGATTTGAGTAAAATGACCAACTTGTTAAAGCTGCTGACAACTAAAAACATCAAAGCAAAATTGGGGAAGGGAACAGCAATGCACAAGACACCTTCAGCTTTGTTGTTCCTTATTCGGGGAATCATACAAATTCAAGTGGTTCATCAGGAACCATTAAAACTGCTTATCCGGTGAAAAAAGATGAAGCTTCCCAAGTAGCGATCAATTCCTTGATCAACGCTACGCCGTTGAATAGTTATGGGACAATAAAAAAACTACCTGTCAACAAAAAACAGGTAGTTAATTATTTATCCAATTAGTAAGTTGCCTATGGGTTAACAAAGAGATGCACTTAATATACGACCAACCTAATTTATTTGAAAGCGTATCTTTTATTAATGAATGTGGAACTAAGCAACTATTATTTAAACACAGCAAAAGCGTGTAATTTTTTATTAAAAGCGATTTAGTGGGAACAGAAATAAAAGATTAACATGACTTTATACAACTTCTGTTCCCATCTACTAAATTGCACAAAAAAAGCTTTTTGACAAAAAAATAGTTATTTTTTGTAATTGCTTAATTTAATAACAAACTAAACTATTATTGTTACTTAATGATTTTTTGATCTATATAGCACAATTAATATAACTTCATGATTGATCAAAACAAGTTAATTACTAAGTGAAAAAAAGCATTTGCAAAAGCTAAGAATTTAACTACTCTAGTTAATCTTAAGAACACTTTACACAACAGTGATTTAAAGCCATTACTCCAAAAGATTAAAACCGCTACAAAACTAAGTGAAAAAAGTAGTTTAGGTAAGCTTTATCAATCACTTGATATTCAACTAACTGATCTGTTAACTAGTTACAAAAAAACCTTTGAAATAAATAACCAAGTTAGTCAAAAACCTTCACTTGATGTGATGCTACCAGCAACAGAGTTTACCAATGGTTCTAATAACGCACTATATCAGGTCATTGATAATTTAGTTGAATACTTTAAAAGCTTTTTATTCACAATTAATTTTGATAGTGAACTGACCAGTATTAGTGACTGTTTTGATCTTTTAAATATCCCTAAAGATCATTCCAGTAGGAATGAATCTGATTCTTTTTATATCGATAAAACCAGTTTATTGAGAACCCATTGTACTGCTACCACGCTAAAAGCAGTCAGAACTTCTAAAAAAACTAATAATCCTGATATCAGGGTTGTCTCTTTAGGAGCGGTTTTTCGTAATGATAGTGATGATGCCACCCACTCCCATCAGTTTACCCAACTTGATTTTATGTGGATTAAAAAAGGGCTTTCATTAGCTAATTTAAAGTGGTTTATTAACAATATGATCACCCATTTCTTTGGGGAAAATACTTTTACTAGGTTTAGACTATCCCACTTCCCATTCACTGAACCCTCGTTTGAAATTGACATTAGGTGTTGGTTATGTCAAAATGGTTGTTCTATTTGTAAGCAAACCAAGTGAATTGAGATCTTAGGGGCGGGGATCATCCATCCCCAGGTGATGAATAACATGGGAATTGGGGATACTGAAAATATTACTGGGATAGCAGCAGGAATTGGGATTGAACGCTTAGCAATGTTAAAGTATGGGATTGATGATATCCGTGATTTTTATGATAACAACTTTAAGTTTTTAACCCAGTTTACTGACTAAAATATGTTGATATCAAAAAAAACACTTGGCGTTTTAATCCCTGATATCTTTAGTTTTTCTAATGATCAAATTGCCCAAAAGTTAGAACAAATGGGGATTGAAGTGGAATCAATTAAGCAGTTTAACAGCCCTGATTACCTCCAACTTGCAAAGGTTGTATCAATCCAACCCCATCCCCATGACAACAAGCTTTTTATCTGTGAATTACAAATTGATAAAAACAAGTTTATTAATGTTGTTTCCAATGCTAATAACATTAACAATCCTGATAATATCAACAAGTTTGTCATTGTTGCAAAAAAAGGAACTGAGTTACTCAACGGGTTAATTGTTAAAACCCAAAATATTAAAGGGATCATTTCAGAAGGGATTTTATGTAGCTATATTGACATTAACCCCTTCAGTAGACAGATCATTGAAAAAACAGAAGTTGCTGATGCGATTATCATTGATCATGTTAGCAATGATCATGACTGAAACCAATACCTCTCGTTTTTAAGTTTGGATGATGTGATCTTTGATGTTAAAACCCCAACTAACAGAGCAGATCTTCATAGCTTAATCTTTTTAGCAAAAGAACTTGGGGTACTTTTGAAAACCAAAACCTTTTTAAAACAAAAAAGTAGTGTTGTTAACCATGACTTTTTTAAGTTTCCCCTAAATTTAAAAAACAAGTTAAAAGCGAATTATTTTGGCGGTTTGTTCTTAAGACAAATTAACCAACATAGTTCACCTTGAACAGTTAAAGGACTGTTAATTAACCAAATGATCAAACCAGTTAACTATTATGTTGATAAAGCTAACTTAGTAACAGTGTTCACCGCTCAGCCAATCCATTGTCATGATGCAGATAGAATTGTTGGTAACATTGAACTTAAACAAGCAACCCATAATGAAACTTTTGTTGGACTTGATGACAAGCAATATGAGATTGAACCAGGGGATATTGTTGTTTGTGATGAGAAGGGCATTATTGCACTGGTAGGGATCATTGGTTCAAAGCGCACAATGGTCCAACCTACAACAACTAACATCTTTTTTGAAGTTGTTAACTGTAACAGTGAAACCATTAAACAAACTGCCAAGCGCTTTTTGATCAATAACTTTGCCAGTAAGTTTATGGTTAAACCGATTAGCTTATTAGCTACTGATAACTGTTTAAACTACTTACAAAACAGTTTACTAACCACTGATAACATTGGCAAAATTAGCCACTTTTCAAGTTCGCTTAAAGTTGAACCATTTAGTAAAAAGCTCACAGTGAATTTCCATAAGATACGCCAACTAATTGGCATTGAAAAAAAGGAACTAACTGATCAAACCATTAAAAAAAGCCTCAGTCAACTAGGGTTTAAAGTTGACAACCAACTTCTCAAAATCCCCAGTTACAGACAAGACATTAATACTTGACAAGACATTAGTGAAGAGATTGTGAAGTTAATTGATATCAATAAGTTAAAACCAATTGGGATCACTAGTAGTTTTAACTTTGAAAAGTCTAGTTACTTTAACACTTTTAATGCTTTAACAAAACTAAGAAAAAAACTGCAAACACTTGGTTTTCACAACGTTATTACCTACCAGTTAACTGATCAAAAAAGTGCAAAAACTTTTAATTTGTTTAACTTAGAAAATTTCATCACCATTAAAAACCCAGTGTCCCAAAACCATTCTGTAATGCGTGTTAGCTTAATTGATTCACTGTTAAAAGTGCTACAAACCAATAACAACTATAAGAATGAACTGGTGAACATCTTTGAGTTTTCCTTTATTAAAACCCAAAACAATAGTGAACTGCACCTGGCAGTATTATGAGTTGAAAAACTGTTTACTTCTAGTTTCAATCCTATGCAAGGGATAAGCAATGATGTTTTTACTATGAAGGGATTAGCAAAACTCATTGTTGCTAACTTAGGGTTTAGTTGTGACTTTGAACCACTTGATGATAGTGACTATTTTGTTAATAATCAAAGTTTAAAAATAGTAGTTTTTAACGAACAGATCGGTTTTATTGGGCTAATTAAAGAATCATTGTTAAATAACTATGATCTGAACAATAAACCCATTTATTGTCTTGAAATCAACTTAGATAGGATGCTCTTTTCTCTAAACAGGATTGAAAAAAACTACCTTGGTTACAGTAAACTACAACCTGTTTGCAAGGATCTTACCTTTAGTTTTACCAACCCTGCTAGTCACTTTGATCAGTTTGCTAACATGATCAAAAGGATAACTGGCATTGAAAGTTGAAAGTTAATTAGTGTCTTTGAAACTATGCAAAACAACCAACTGATCACTAAGTACACCGTTCGTTATTTTCTGAAAAATGATGCTAACAAACCACTAACTAACCAAACAATTGAACTTATCACTAATAACTTAAAACTCCAGTGTGAAAAACTAAAAATTAAATTAGATATTTAGAATTACTTATTACTATCAAGATAGTTACCAACTATTTTGTTAGTAAAACTTAATACAATTGGCACAAAACGCAAAACACCCTTCTAAAAAAGAACAAAAACCACTCGTTAATGAACAGATTGCTTTCAATCAGTTCACTTTAATTGATGAAAACAGTACTAATTTAGGGATAGTTAAGATGGAAAACGCTTTAAAGTTAGCACAAGAAAAACAGTTAGATCTAGTTCTAATTGCTCCAAACCCAACCAAACCGATCGTTAAGTTGTTGGACTTTGGCAGATATACCTATGATTTAAAGCGTAAGAAAAGACAAGCCAAGAAAAACCAAACAATCATCCAAACCAAAGAAGTTGTTGTCAAACCAACGATTGCTAAACATGATTTAGAATTTAGAGCAAAACAGAGTAAGAATTGGATAGAAAAAGGTCATCATGTCAAGTTTATAGTCCGTGCCTTTGGCAGGGTTAGCACCAGGATAGAGTTAATTGAAAAGGTGTTTGATGACTTTTACCAGTTAGTTAAAGATGTAGTTGAGATCCAAAAACCTTTAACCGCTTCTTCCAAAACGATGTACGCTGCTCTATTAGTACCTTTAAAAAGATAGTTATGAAAACCAAAAGTGCTGCAGTAAAACGCTTTAAACTCACCAAATCAGGACAAATTAAGCGCAAACACGCTTATACTTCCCACCTCGCGCCCCACAAATCAACCAAACAAAAGCGCCATTTGCGCAAGCAAGCTACTGTGAGCAACAGTGAATTGAAAAGAATTGGTATTTTAATTTAGTTATGCGTGTTAAGGGAACAAATACAACCAGGATTAGAAGAAAAAAATGGTTAAAACAAGCTAGTGGTAGCTTTGGGACAAGAAAAGCTTCTTTTAAAGCAGCTAAACAAACTGTTATCCAAGCAAGCAAGTATGCTTACCGTGATAGGAGACAGAAAAAACGTGAGTTTCGTTCGTTGTGGATCTTAAGGTTAAATGCTGCACTGCGTGCACAAGGGATGACTTATTCAGTGTTTATCAATGAATTGAAAAAAGCCAAGATAGCCATTAACAGAAAGGTACTTTCTGAACTAGCAATTAAAGAACCTAATAAGTTAAATCTGATTATCAATACCATCAAAAAACCAACTAATAAACCAACTGTTGCAAAAACTTAGTAACGTTTTTAAAGTTAAGTTTTGCCACCTGTTTAAACTGTTCAATTTGACTAAAACCGCGTTGTTGGTTAAGAAATAACAACGCTTGTTCAATGCCATGACTACTGCCTTTGGGTAAATCATAGTTTACCTTTTGGTTGAGTAATTGCAGCTGATTAATAAAGCTATCTCTTGCTAAGATAGATGCAGCTGCAATCACCAGTGACTTAGTTTCACCATTAATTAGAAACTGATCAGGTAATAACACTGTTTTATCAGTGAAATTAGTTAGCTTATTTAGATAGTTAACAAATAACTCTTGATTGGCAAACTGGTCAATGCTAATTGTTACTGTTTGTCTTAATAACTGATTTTTTTCCAGTAACTTTTGGTAAAGCTGACAGTGTAAATTGGTCAAAAGTAAATTGGTATTTTTCAGTGATTTTGTCAAATCATTATACTGCTTGGGATCTAATGTAATTGTATGGTGATCCATTACTGTTGTTTGGATCGATTTAGCTAACAGTTTGACAGTGTGATCACTGAGTTTTTTTGAATCAGTTACTTGTAAATTTTCAAGATTAATAAGCTGTTTTTTTCAATTAAAACAGCACTAACACAAATCCCACCAAAACTATCACCCTTGCCAGATTCATCACTGCCAATTAAATAAAAATCAGCTGGTTTGTAATGTTGCAACTATCTAGTTAATTAATAAAATTATACTTACTCTGATTGATTCTTAATGGCTGAACAGAAACGTGATTATTATGAAGTGTTAGGAATAACCCCTGATGCTGATCAATCAGAGATTAAAAAAGCCTTTCGTAAGCTGGCTAAAAAGTACCATCCTGATCGTAACAACGCGCCTGATGCTGCTAAGATTTTTGCTGAAATTAATGAAGCAAATGATGTTTTATCAAACCCCAAAAAAAGAGCTAACTATGATAAGTATGGTTTTGATGGGGTTGATGGCGAACCTGCTTTTAACTTCCAAGCAGATGTTTTTCAATCCTTTTTTGAAGAGATAGCAAAATCAGGGGTTTTTAACAACCAAACCAATCCTGAACAAAAAGAAAAAAGAAACGTTACCACTGGTTCTCCAAAAAACCTAAGCAAGAACAACCTGAAATTAACTTAGATCACGTTGTTGAGCAAACCATTAAAAAGGTGCAACAAAACCAAAACCAAAACAAAGACCCAGATGAATTACGTTCTAAGGTCCCTGGAGAGGTTACTGCTAGTGATTGGGAAGCATTGGTTGGTGATACTAGGTATGGGTATTTTGATGAAACAGGGGATTGGAGTTGAAAGGGTTACTTTGATGAACAGGGCAAATGGGTTTGAAACGAACCAGTTGATTCTGAAACCAGTGAGGTATCAGTTGAACCTGAACCAACCCCAGTTGCCCCTGAAGCTAGTTTTGAAGAAGCTCAACCTGAAACCAGTTGCCCCTGAAGCTAGTTTTGAAGAAGCTCAACCTGAACCAACTCCAATTCCTGAACCAATCCCAACCCCAGTTCAAGTTCAACCCCTGTTGTTAGATCTCAACCTCTTTACTATCCCAACTAAAGCTACTAAGGATGATCTTTTGTTTGACAACATTAACCTCACTACCTATGAACAAGTTGTTGATTATCTCAACAGTCAAGCAACCCCTAATTTAGCTAAAACCGATGGTGAATTGCAAACGATTGATGGTACCAACCCATTGTTATTAGAACAGTGCAAAAAGATCAAAAAACAAGCAGAACAACTCTTTAAAAAACTCTTTTTAAAAAAACAACTCCCCTTCATCACCCAACCTGAAGTTGTTGAGGAAAGTAAAACCAGTTTTGATGAGAACAACGTTAACCTTGTTTACTTTGAAAAGGTCCCTGAAATCCTTTTCATTAACCAACAACCTAAGGAGGTAAAATACACCCGTCAAGTCTTTGATGGGTTGACAAACAAAACAACTAGTGAAACGATTACACTAGAGATCCAACTACTCCAAACCCCAAAAGAGACTGTTAGTGCCATTTTTAAAGGCTTTGGTAATGACCATGGCAAGGGCTGTGGGGATTTAAAGATTGTTTTTGAAAAGATTAAAAGCCCCTTTTTTCAAGTCAATGAGGATGGCTTGCACTCTGCTTGCATCATTGACCCTTTAGTTGCTTACAACGGCGGGATTATCGATGTGTTTGGGCCCTACACTAACTTCCAAGTTAAGGTAGATGGGGAGATAGATATCAATGCCATTATGAAGTTTGAAAAACTAGGCATTGCTAAAACCAAACGCAAGGGCGATCTTTTTGTCCATCTCTATTACAGTAGTGTCCCTAAAAAGAAACTCACCACTAACCCCCAAGTTCAACAGTTCTTAGAACTTTTACAAGCTGAATATGAACTGTTGCAAGACAACATCAAGAGCTTAAAGTACTTTAAAAATAACCTAGTTATCCCCAAAAAGCCACTTGATCAACAAAGCTATCAATACCTCTCCCAAGAACCCATTAGTTAGAATTTGTTAATATGTGTGAAAAATCACAAACAATTAAAGAGCTTTTAAACGCCATTAGAACCTTAGTTGTCAAGAACAATAAAGCTAAGGTTAGTATGATTGAAAAGGAACTGTTAGCTTTTGTTAGTGAACTTGACAAAAAGTTCAAACAACAACTCAACAACTTCAATGAACTACAACAAAAGATCCCACTACTCCAAAAAGCTAACGAAGAGTTTGCTTTAAAGTTTGAAAGGATGCAACGCGAAGCACAAAACCAGATCCAAGCCAAACTAGATGAGTTGAATCTTAAAAATAAAAAGGAGTTAGAACAAGCCAAGAAATATGCGATTGCCAAAACCCTTGACCAACCCTTAAACATCATCGATCAGTTTGAAATCGCGCTTTCATATGCCCAAAAAGACCCTCAAGTAAAAAACTATACCACTGGTTTTACCATGGTACTTGATGCTTTTTCAAGGTGATTGGAAGCAAATGGGGTTACCAAGATTAAGATTGAACCAGGGATGGAATTTGATGAAAAGATTATGTCTGCATTGGAACTAGTTGATTCTAACCTTGCTAAAAACAAGGTAGTAAGAGTCTCAAAATCTGGCTATAAACTCTATGACAAAGTGATCCGCTTTGCATCAGTATTTGTCAGCAAAGGTAATAAAAAATCATAATAACAACTTATGAGTGAACAAAAAAGAAGAACAATCCAAATTGCGATAAGTGAAGACCACTATGAAGAGTTACAAAAGGCATTGGAACTACTTAAAGGGACCCAATTACCCTTTTCAACCACTGTTGAACAGTTTGTGGAGTTAATCTTATCTAACTATGTAGCTACTTCCAATAAGATTAGTAGTTTAGCTAAGAGTGGTTTTGATGTAGCTTCATTGCAGCAAGAACTTGAGAAGATAGGTAACCTTAGTGGGGTTGATGATAACCTCAAGGGTTTTCTCTCAGAACTGTTGAAAACCTCAAGGAATGGGTTTAGTAACCCCAATAAAGATGGCAAAAAAAATGATGACGATAATAACTCGTCATCAAAATCATAGTTATATAAAACTTTAAGTGGTGCCGAATAACAGAGTCGAACTGTTCTCTGATCCTTACCATGGATCTGTTTTGCCCCTAAACCAATTCGGCAGCAAAGCTAATTTCAATTTTAAATTCAAACTTTAGATGAAAAGTAACTACAGTGCAACTAACATCAAGATCTTAAAGGGTTTGGATGCAGTTAAAAAGCGTCCGGGGATGTACATTGGTTCTACTGATAGTAAGGGTCTGCACCACATGCTATGGGAAATTCTTGCTAACAGTGTTGATGAAGTTTTAGCTGGTTATGCAACCAATATTACTGTTACTTTAGATCTCAACAACACCATTACTGTTAGTGATGATGGCAGGGGTATTCCCTATGAGATCCACCAAGACAGTAACATCTCTACGATCGATACAGTTTTCACCTTTCTCCATGCAGGGGGGAAGTTTGATGATCAGTCATACAAACTAGCAGGGGGATTACATGGGGTTGGTGCATCAGTGGTCAATGCCTTAAGTGATCATTTAGAAGTAACAGTGAAAAGAAATGGTCAGATCTACCAATCAGTTTATCAAGCTGGGGGTAAGATCATCCAAAAAGCCAAAAAGATTGGTGATACAACTAGCCATGGTACCACTGTTAGTTTCCATGCTGACCCTAAGGTCTTTAAAAAGGCTCAATTTGATAGCAACATTATTAAAAGCAGGTTAAAAGAGCTAAGCTTTCTGTTTGCTAAACTAAAGCTCACTTTTACTGATCAAAAAACTAATAAAACCACTGTTTTTTTTAGTACCTCAGGACTAGTTCAGTTCCTTGATGAAATTAATAATACTGTAGAAACACTTGGCCAAAAAACACTGATTAAAGGTGAGAAGGATGGGATTGAAGTGGAAGTGGTTTTCCAGTTTAACCAATCAGATCAAGAGACAATCTTATCATTTGCTAACTCGATTAAAACCTTTGAAGGAGGGAGTCATGAAAATGGGTTTTGTCTTGCCATTAGTGATGTGATCAACAGCTATTGCAGAAAGTACAACTTACTAAAAGAAAAAGATAAAAACTTTCAACTTAGTGAGATCAGACAAGGGTTGAATGCTATTATCAAAGTTAACTTACCTGAAAAAAACATCGCTTTTGAAGGACAAACTAAGAGTAAGTTGTTTTCAAAGGAAGTGAAAAACGTTGTTTATGAATTGGTCCAACAACACTATTTCCAGTTTCTGGAAAGAAACAACAATGATGCTAAATTGATCATTGATAAACTACTCAATGCTAGAAAGATTAAAGAGCAAATCAAACAACAACGTGAGTTGAAAAAAAGTTTATCAAGTCCCCAAAAAGAGAAGATCTTATTTGGAAAGTTAGCACCTTGTCAAACCAAAAAAACCAGTGAAAAAGAGTTGTTTATTGTTGAAGGTGATAGTGCTGGTGGCACTGCTAAAATGGGCCGTGATAGAATTTTTCAAGCTATCTTACCTTTGCGCGGCAAGGTGTTAAATGTTGAAAAAATTAACAATAAGAAGGAAGCGATCACTAACGAAGAGATCCTCACTTTAATCTTTTGTATTGGTACAGGGATTTTAACTAACTTCAACATCAAGGACTTAAAGTACGGAAAGATCATCATTATGACTGATGCAGATAATGATGGCGCACACATCCAAATCCTCTTACTTACCTTCTTTTATAGGTACATGCAACCCCTAATTGAACTGGGCCATGTCTATCTAGCTCTTCCTCCTTTATATAAGCTAGAAACCAAAGATAGAAAAACAGTTAAATACCTCTGGAGTGATTTGGAGTTGGAATCAGTCAAACTAAAGCTTAATAACTTCACTTTACAACGATACAAAGGACTTGGAGAGATGAATGCTGATCAGTTGTGAGATACTACTATGAATCCAACTACCAGAAAGCTAGTGCAAGTAAAGCTTGATGATCTAATTAACGCTGAAAAGCAAATCAACATCTTTATGGGTGAAAAGAGTGATTTGCGCAAACACTGGATTGAAGCCAACATTAACTTTAGTATAGAAAACTAATGGATCAAAAAAACAACAACCTCTTTCAAAAAGCAATTGAAGAAGTCTTTGCAGTTAGCTTTAGTAAGTATGCTAAATACATCATCCAAGATAGAGCTTTACCTGATCTAAGAGATGGGTTAAAACCAGTGCAAAGACGGATCTTATATGGGATGTTTCAAATGGGCTTAAAACCCACCACTTCCTATAAAAAATCAGCCCGTGCTGTTGGGGAGATCATGGGGAAATACCATCCCCATGGTGATAGTTCCATTTATGATGCAATTATCAGAATGTCCCAAAGCTGAAAGAACAACTGAACAACTGTTTCTATCCATGGTAACAATGGTTCAGTGGATGGGGATAATGCTGCAGCAATGCGTTACACAGAAACCCGCTTAAGCTTGTATGGATTTGAACTATTAAAAGACATTGATAAAAAGCTAGTTAGTTTTGTTAATAACTTTGATGATAGTGAAAAAGAACCAACGGTTTTACCAACCTTACTGCCTAACCTCTTTATCAATGGTGCGAGTGGGATAGCTGCTGGATATGCAACTAATATTGCTCCCCATAACACTAATGAACTATTAGATAGTCTTTGCTTGCGAATAGATCAACCTGATTGTGAACTTAAACAAATTTTAAAAATTGTTAAAGGTCCTGATTTTCCAACAGGGGGTAATGTTTATTTTGAAAAGAGTTTAAGTGATATTTATCAAGCAGGCAAAGGTAAATTTATTATCCAAGCTAAGTATGAAGTTAACAAGAACTTAAACCAGATTGAAATTACCCAAATCCCTTATGAAACACTGAAAGCTAACATTGTTAAACAAATTGAAGAGATTATCTTTGACAATAAACTCTCTGCTATTGAAAGTGTCATTGATAGTTCAAATCGCAATGGCATTAGGATCATTATTAAACACAAGGACTTTTTGCCTGCTGAGAAGATCATGGCCTTTTTGTTTAAACACACCCAACTCCAAGTGAACTTTAACCTTAATAACACCGTGATTGCTAACCGCTTTCCCATCCAGATTGGTTTACTAAGTTACCTAGATCATTTTTTAAAGTTTGGTCATGAACTAATTATTAATAAAGCTAAGTATGAACTTGAGCTTGCAAGCAAGCGCTTGGAAATTATTTTAGGACTAATTAAAGCGATTAGTATCATTGATAAAATCATCAAATTAATTAGATCAGCAGTTGACAAAAGTGATGCAAGACAAAAGTTAATTGATAACTTTAAATTTACTTTTAACCAATCAGAGGCAATTGTTAGTTTACGACTCTACCAACTAACTAACACTGATATTTTTGAACTTAACCAAGAACAAAATGAACTTGAAAAAACTGTGATTAGTTCAGAGCAACTAATTGCTAGTGAAAAAGCAAGAAACAAACTCCTAAAAAAACAGTTTGAAGGTTATAAAAAGCAGTTTCACCAGCAACGAAGGTCACAAATATGTGGCTTTATTAACCAAAAAAAGGTGGAGGAAAGTGAGCTAATTGAAAACAAAACTTATGGGGTTTTAATCACTAAAGCTGGTAACTACCATAAGTTTGAATCTAACCAACTATTAAAAAGCACCACTGATTTTAAAAGTGAGAGTGACACAATTATCTTTGCACAAACTATTGCTAATACCGACCAAATTTTTATTGTCACTTCACTAGGTAATATTATTAATATCCCTGTTTATAAATTAGCTTTCAATTCCAAAAATAAACTAGCAAGTTTAGTTAGTAAAAAACCAATCCTTTTGGAGTATGAAACGATTGTTTTTGTTGGAACAATGAACAGTGTAAACCAACCAATCCTTGTTTTAACTTCCAAACTAGGAATGGTTAAACGGATTGATTTAACCAAACTTAACATTAAGCCACTTAAAGCTACTTTGTGTATCTCACTCCGTGATAAAGACCATTTAGTAAGTGCATTTTTACAACAAGATGATAAACTGATCTGTTTAGTGTCTGATCACAACTATTACACTGTTTTTCACACCAATGAGATCCCATTAATTAGTAGTAAGGGGATGGGAGTGAAGGGGATGAAGTTAAAACTAGAGGATCAAATTAAGTTTGTTGTTGCTTTTGAAGCTAATGAACCGTTAGTGATGATATGTAGTGATGGTAGTGTCATTAACTTAAAACAAACTGAACTAGTTGTAGTTAGCAGGATGGCAACTGCAAAAAAACTGCCTGTTAAGAAAGCAATTAACTATTGTTTTAGTGATGCAACTAACACCCAGTTAATTAATTTTCAGGGTAAGAACGGTAGTAAATTAATTACAACTAGTGAACTGAACCAGATGAGTAAAACTGCAATTAGTCAAACCAGGTTTAACAAACTTAATTAGGTTTGGTTGTGGATCTTTTTATCATATTTTTCTATCTCTTCTTTTAGAGTAGTTAATAAGGCTTTAATCTTTTGGTAGTCCATTCTGGTTGGTCCTACTATAGCAAGTTGGTGCTTGGCTTCACTGGTGGTATTGATTAAAGTAGAAGCGACTGATATCTCTTTAAAACCCAATTGATCACCAAAGACAATGTTGGTCTTTTGGTTGGTTTGGTTTATGAATGCCATCTGTTGTCAAACACTAGTATCTTCAAGCAGATTTAAGATTTTAGTGAGTTTCTCTTGGTTAGCAAACTCGGGTTGTTTAGCAAGGTATTGGATCCCATAGATCTTTTTATTTGCCTCAATTTGATCTAAATCAAATAACTTAAATAAGATCTCATCAATTACATACTGATACTCATGAACCTTGGTTCTAATGATCTCTTTTAAAACTTCTAGTTGGTTGTTAATCTCTGAAAAGCGGGTATCAATGATGCGATCATTAAAGATCCTTACACAAATAACAATGTCTTCAAACTGCCTTTGGTTAGCATAGCTAATGGTTTTTTTAAAGACTTTACCACTAGCGGAGACCAATAAAAATAAGGCAAATGACTTATCAAGGATGATCAGATCAATCTTTTTTAAAACTTCATCACTACTAAAGTTAGTTAACACTACTGGTAGGTTAATGATCTCATTGATAAACTTAACCCCAAGTTCAATCACCTCATCAATCGTTTTGTGTTGCTGGAGAATAACACTTCTTAAGCGGGTTTTCAGATCGTTAGTGGTGTTACTAACATTTAACACTTTGACATAATACTGATAACCAATTTGGGAAGGAACACGGCCTGAAGAGATGTGGTTTTTCTTTAAGAACCCTTTTTTCTCTAAAGCTGCCATCTCATTTCGTAATGTTCCCCCAGAGAGGTTTTTAAAATATTTTTTGGTTAGTAATTTACTGCCAACAGGGATAGCATAAGCAATGTATTCATTGATAATTGCTTTGAGAATTTGGGCTTGCCGTGGCGTTAAATTCTTCATTAGTTATCTAATTTTATCTTTTAACAATAACCAGCTTGACCACTAATCTAAAACAAAAGTTAAAAACTGCACCTAAAAAACCGGGGTGTTATTTGTGAAAAGATAGTAACGGTAAAGTTTTATATGTTGGTAAAGCTAGTAATATTTTCAACAGGGTCCACCAGTATTTTCAAAAGAATAATCCTTATAAAACCCAGCTATTATCAAGCCAAATTAGTGATGTTGATTTTTTCATTCTCAAAGATGAAAATGATGCTTTAAATCTGGAAGCAAAGCTCATTAATCAATATCAACCCCGCTTTAACTTAGTTTTAAAACAAAACAATGGTTATCTTTATTTTTATATCACTAAAGCCAAAAAACCTACCTTGGAATTAGCCAGAAAATACCAGATTAAAACCACTAAGTGCTTTGGACCGTTTGCTTCAAGTAAGTTTAAGTTACGTGAGATCCATGACCTACTTTTAAAACTCTTTCCTTTAAGAAAGTGTGCACCTCACCAAAAAAACCACCCGTGCTTTTATTTTCAGATGGGTTTATGTATGGGGCAATGTATGCAAACTGATACTAAGGAAAAATACCAACAGGTAATTAGTAACATTGAACAGTTTTTTAATGACCCTAGTGTGGTAATTAACTATTTAAAAGCTGCAGAAAAAAAGGCAAGTGATAATCAGGAATTTGAAAAGGCCCAGCAGTTTCTAACACTGCAAAAAGCAGTTTTAGAGTTAACAAAAACCCACCATACCACTATCATTAAACAAAAATCAAGCCATGATTTTATTGGGTATGTCTTTCAAAATAACGTTTTGGCCATTACCATTTTTTGTTATGAAAAAGGGGAGTTAACTGATAAAGAACAAGCAGTGTTTACCCTAGAGCAAACTGACATTGTGGAAGTTGAAAGTGCTATTATCACCTTTATCTACCACCACTATAAAACTACCCCACTTCCAAGTAAGATTACTGTTTCACTTGATGAAACTAACCTAAAACTTATTAGTGATAGCTTAAAAATTGGTGTTTTTAAGCCCAAGGATGGTAATGAAAAACTGATCTTACAAACTGTTATTGATAATGCCAAACATGCACTTGCAACCAAGTGGTTGAAGTTTACTAGTAACTATGATAAAACCCGGCTCCACAAGGATTTAGCACAACTTCTAAATACTGATTATATCCATAGTCTTGAGATTATTGATGTGTCATTCTATGATCAAAACCATGTTGTCGGTTGCATGTTAAGGTTTGAAGATGGTAAAAAGATCAAACACTTATCAAGAAGATACAACATTAACAGTTTAAAAAAAGGTGATACTAACCACATTGCTTTACTTGTTTACAGAAGGATCTTAAGTGCGATGCAAACCAAAGCTAACCTCCCTTTTAGTGATCTTTTAATTATTGATGGTGGTAAAGCACAAATTAAAAGTGTTAAGCAAGTTTTTAGTCTCTTCAGTAATGTTAAACCACCCATTATCATTGGACTAGTTAAAAACAAAAACCACCAAACTGATCACATTATGTTATCTGATTTCCAAGTTAAAAAGATAGCAATTAACTCCCCACTCTTTCACTATTTAGCAACAATCCAAACTGAAGTTGATGGTTTTGCTAAAAGAAGTGCTTTTAATAAGTTAAGTAACCACCAACTGCAAAACCCGTTGCTACAAATCCCAGGAGTTGGCAAGATAACTGCCCAAATTCTCTTTGATAACTTTCAAACGCTCAATAACATAAAATTAGCTTCAGTTAATGAGTTAAGCCAGTTTATTAAAAAACCATTAGCACAAAAGATTAAAACTTACTTTGCAAAACAAACTGATTAAGGTTTTGGTAATTGCTGATACCCATGGTCAAAACCAGAGGTGGATTGAACTGAAAAACTACCATAACCCTGATGTGATTATCCATGCAGGAGATCACATGACCACTAAACAATTCATGGATCAAAATGCTACTTTTTGAGTGGCAGGTAACAACGATTCAATTGGCAATGAAATTGAAATTTTCCAGTTAGGGCAAATTAACTTTGTGTTAATGCATGGTCACCAAGCACCAAGAGATAACCTGAAAAAGTGGTACCAATTATTAGTTTTAAAAGCACAACAATACCCTTGTGATGTTTTAATCTTTGGTCATAGTCACATTGAATATACCAACAAAATTAATAAGATCCAGTTAATCAACCCTGGTTCTCTACAACTACCAAGAAACCAAACCAACACCCCTTCATACTGTACCTTTATTGTCAATAAAGACGAGCTAACTGATCTAACTATCCACTATTACCAAGCTTCAAAAGTTAGTTAACAGGGATCTTTTAGATAGATAGGTTGTAAGGTTAGAGGATCTTCGATTCTTTCAAAGTGATCGATGTTGCTTAATAACAGCTTGCTATAACTCTCAATGTTTTCAAAGTTTTCATACATAGGAAGTTCATTGTTTGCCTTACATAACTTCACAAAATCAGCTTTACTAATTAGCTTTATTTCACTAGTAGTTTGGCTGTATAGTCCACAATAGTTCTGATCATTACCACAACTAATCTTGCTAATCCCATGTTCATATGGGATTTGAAAGCGGAGTGAATTTAAAGCATAAAGTTCACAACTGGGATACAATAAACACCAGCTTTTAGCAATGATAGTTGCTATCCTCTGACCGGTGAAACTACCAGGGCCAATTGTGACATAAAACTGTTTAATACTGCTTTTTTTCAACTTGTTTTTTGTTAACATTGTCTCCAGATAATAAACAGCAAGTTCAGTTAAGTTCTGTTCAACACCAATGGACAACTCATCAACCACTGCATTGGTTTTCATCTCTAAAATAATGATGTTAAGGGTTTTATAAGCACAGTCTAAAAACAGCTTATACTTACTTAAAAAGAACATGGAGTGGATCGGTTAAAGATAAGTTTAAACTCTTAAGTTTCGTTGAAAACTTAGGTTCAAGTGGGGCGTGAAAGTCCATAGGTTTGTTTAAAGTGGGATGGATAAAACAGATCCTGTTTGCATGGAGAAACTGACCATAGCTATCTTTCTTCTCACTTTTAATTCCATACAGTGGATCATTATAAACTGGATGTTGGATAAATTTCAGATGCACTCTAATTTGGTGGGTTCTACCTGTTAACAATTCCAAGCTAATTAGTGCTGCTTTTTCATTCTGATTAATCACTTTAAACTTAGTTATTGCTTGCTTTGCTTTAGCAGTTTGGGCTATTTTAAACATTACCTTGTTGTTATTAACCCTTGCTAAAGGTGCATTAATTGAACCAGTTAAGGCATTAAAAGGGAAGTGGACTAGTGCTACATAATAACGTTTTAAGGTGCGATTTTGCAGTTGATTTTGCAAATTTAATAAGCTTGCTTGGTTTTTACAGACAACAATTGCCCCACTAGTATCACGGTCCAATCTGTGCACTAAGTAAACAGGGTTTTTGTTGTTGTGAAAGATACAAGCAGCTAACAAGCTGGCTTTTTCATTGAAAGTGGTGGGATGGGTTAACAAACCTGATGGTTTGTTAATAACCATCAAATCCTTGTCTTCAAAAAGAACCTCAAGCTTTAAGTTATAAGGTTCAACACTAGTAATGAAATCACTAGTTGTCTCATCATGAATCTCAACTTTAATTACATCATCTTTTGCAACTATTAAACTGTTTTTAAAAGTTAGTTTTTCATTAACTTTAATCTGTCCATTCATGATCAGCTTCACTACCTTTACTCTTGAAAGGTTCAGTAAGCTAGCTAAAAGACTATCTAAGCGTTTGGTAGTTGTAACAACAAAACACTGTTTCATTACTGGTTTTTGGAATGTTTAAACTCTTTGAACATCTGGATTAAAAACAAAAGAATAAACCTATAAAACCAAAGGTAATACAACAATCAGCAAAGTTAAATACTGAACTACCATTCTGAAAGATAAAGTAATCTAACACTGAATCATTAGCTGAAGTTAAACGATCAAAGAAGTTTCCAAGTGAACCAAATGCTAAAGTTGTAATTCAAAAGATGTAACTATATTTCACCATAAAAACAAGAAAAACTAACGCAATTACTGATAATAATCCCTGGAGAAAGTAAACTAAGCCAGTTTGGTTTTGTAATAAGCTAAACCCTACCCCTTTGTTTCTAATCACATAGATATTAATAAAACCACTATTTGCTACCATGGTGTTATCCATCTCACCATTTAACGCATTTTTTAATATGAAAACTTGCAGTAAGATAATAAAGCCAACAAACCCAATCATTGTCAGTTTATAGAATAAAAATGGTTTTTGGTTTGCAGTTAAAACCTGGTGTTTAAGTTGTGAAAAAAACTTGGTTTTTCTTAATTTCATCTACTTGTATATTCAGTTAAAATTCACTAATTAACTAACAAAAATTATTAAGGAGTATTAACACTTTTTTACTAGTTTTTAAACTAACTTTTTTAAGTGTAATCCTAGTTTAATTTCTGGTAGTTTTCTTTACTAATAGCTACTTGTAAAAAATTAAAAGTTAGAATTAATGGCACTATTTAGTATATAGATATCAGGCTTTGGGGAAAACAAAAAACAAATCAGACTGACAGATCTTTCTAGAAGATTACCGTTTTTATTTTGAAACAGATTTTGATTGGGTTACATACCTGAACAACTGTTTAAACAGCTATCCTGATTTTGACATCATCAAGTTCATTAAGAAGTATGGCCCTGAGTGTGAAAAGAGCTTTCTAAGCTGACAGAGCAAAGCTAAGAGTGATGTTTACAATGAACTGGCTAACAAGATTAAAAAACAACAGTTTTCAGAACAGTTAATTTACCAGCTAGTCCAACTTGATGCTTTACGAACTAACTATTTAATTGGTTCGTTGTTTTCAGATAACAAAACCCAGCGCAAACTCCTGAAGCGTTCTTGAAAAAATGCTAAGAAAGAAGGTTATACAAAACAAGAATGGTTAATGATCTTAGTTGGTTTACCCTTTGAAAAAGGTGCTTATCATAAGCAGTTATATGACCATTCACGTCAGGAGATCTTAGATCTTACTGAAGTTATTAAAAAGCTTTATCTAAAAACAGAGACCAACAACGATAAGCTTGAGTTTGCTGCAACTACTAGTAAAACAACAGCGCAGCTAACTAAAACTATGCCCTTAAACAGTAGTGATCTTGATAAGGATCTAATGGAGTTTAGTGGTGAGAAGTGAGGTGATAATTAGTGCCTAAGATTGAAGTTAAAAATGATGATTTAGAGCTAGCTTTAAAAAAGTTTAAAAGGATATCACTGGAAGTACGCAGGTTAGCACAACGCCATGAATACCACTTGCGCAAAGGGATGCGGTTAAGAGAAAAACAAAAGATAGCACAAAAAAAGCGCAGGAAGTTTCGCAGTTTAGCTAGCCATTAAGATGGATAATAAAAACCCCCAGAAACTTATTACTAGTGAATTGTTGGCAAACCACCGCTTTAATTTTGCTAAAGATGATAAAGGTGGTTATGATGCTAATGAAGTTGATGCATTCTTAGACCAGCTAACCAAGACTTTAATCCACTATGAGGAGATGAAAAACAACGAACAGGAGTTGAAAAATGCTTACGACAAGTTGTTTTCAGATCGTGATCAGATTTTAAGTCGTTGTGCTAAATTAGAAGCTGATTTAAACACCTTTTATGAAAATGGTTATGCAAACAAGGTGCTAATTAACCGGGTTCAGGAGTTGGAGGATAAACTTGAAAAACTACCTGATCGTTACACTGAAAAACTAGAAAGGATTGAAAAACTGTTAAAAAAGGTCATTAAACACTGAACTGATGGGGAGGACATTAGTAACTTTGAAGATGAGTTCTTTTAAAGATGGTTGTTGGGATAGGGATTGATGTTGTGCAATTAAAGCGCTTCTTAACTTTAGTTGAAACTAGTGATTGTTTTGCTAAACGATTGTTAACTAGCAATGAACTAAACAGTTATTGAAAGCTAAACAATAACCAAAGAGCTAATTTTCTAGCAGTGCATTGAACTTTAAAAGAAGCGATTTATAAAGCTACCAGTCACATCAAACCACTTTTCACTAAACTTGAAATTTATAAACTTAACAACCAGTACCGGTGTGAATTTATCCAAAACATCAACCTGTTGTTATCAGTTAGTTACACTAATTGCCATGTTAGTGCTATCTGTTTAGCACAACAAAATGGATAAACTATTTAAAACAAGTTTTAGATTCATAATAAGGTTTTTACAAATCCTGAGTTTACCAGTTGTTTTTCCTTACTTTTTATTAAGCTTTTTAGCTTGTTTAATTACTAGTAAAAACTATGAATCACTCCCTGACAACTATCCCCCTGAAATCCGGTTCAAAAAGGTGTATAGATTGGTATCAATGTGACTTTACATTAAGGGAATTAAAGTAGTGACAGTAAATGACAAGATTATCCCTAAAAAACCAGTTTTAGTGGTAGCTAACCATAAATCTAACCTTGATCCTTTAGTATTAATTAAGGCCTTTGGTAAGTTGAAAAATAGTCCACCATTAACCTTTGTTGCTAAGATTGAACTGAAAGATACAGTCCTTTTTAAACTGATGAAATTAATTGATTGTGTTTTTATTGATCGAAAAAACATCAGACAAATTGCCAATGCATTGGAAACCCAACAACAACTAATTCGCAAGGGCACTGCTATTGCTGTTTTTGCTGAAGGGACTAGGATTTTAAGTAATGACATTGGAGAATTTAAACCAGGAGCACTAAAGGTTGCTTACAATGCTTTTGTACCTATCTTACCAGTTAGTATTGTGGGTAGCTTAGGAAAGATGGAATCAAACAAAAGGCTAAAAGAACATGGTGTTAAGAAAAATTCAAACTATGAGGTTAAAGTAATCTTTAACAAGCTAATTAACCCAATTAGTTTTAACCAGATTGATTCTAATAACCTTGCTAATAACATTAGAAGCATTATTAGTGATGCATACACTAGTGAAAAACCAAGCAATGATTAGTTTAATTTTTGATAAAAGCAACAGTTTTAACCCCCAACTTTGTGTTGAACTTCTCAACCACTTTAAAGTGGTTATGCTAACTAAAACCATCGTTGTTGACTATCCCAGCTTTCAAGCATGAAAAGCACAACTCAAACCGTTTAAATTAGCAGTTTTTAGTGATAATTTGCAAACCGAATTAACCCCAAATTCAAAACTCACAGTTTTTAACAACTACCAGCAACTTTTAGTTGATAATAATGACCTAATTATCTTTGCAACCCCCACTTTAGTTCAGCTTTTTGATAATGAGATTGACCAGTTAATAGTGATCAATCCCACTAGTAAATCTAAAGATCAGTTTAACTGTAATTGAAACGACTTTGTTTTAATTAAACAAACTAATTTCAAGAACCATCAAGTTGGTTATTTTGATAAAAAAAGTAGTTATGAACCACCTTTCACTGTTGTTACAGACCACTTCTTTGGGAACCTAACTGATTTATTTAGTCTTTTAGTTGATAAAAAGGTAAGTGTAAATGATGTGGATATTGGCTTGATTTCCTTACAATATCTCAATATCATTGCAAATTACACCAATAAAAAAGCCATTGAAAAGATCACTGATTATCTGGTGATTACAAGCAAAATTCTCGCTAAAAAAGCAGATAATCTGCTCAATGATCATCAGGAAGAGAGTGTGTTGGAATATGATTTAGCTACTAATAACTTTCGTGATAAGATGATCGCTAACTTAGTGGAACACAAACGCTATTGTGATTCACTGGGTGAATTTGAAAAACTCAGGGTTAACCGCTTAGCTTACTTTTCCAAAGCTAATGAGATGGAGCAATTTATCAAAACAGCTAATGATCAACTTGTTACAGTTGAAGACCAACTCCCTAACTATATTAGTGTCTTAAAACTCTTTCATGCTATGAACAAGTTACTGGAGATGAGACTCAGCTCTCTTCTAACAAATAAAAACATCACCATTAAAGAGTTATCAGTTGAACAGGTTCAAAAGGAGTTGGTTTTAGCAATCAAACAGTTTAACTACCAAACAGTTTCTTTAAAGCGGGTGTTATTAAAACTTAACCATCCTATCTCTTTAATGTATTTTGTTACTGCTTTTGTGGCACTTTTAGTGCTATTAAACAACCAGGTAATAGGTTTGGAACAAAAAGATTATCACAGTGAACTTTACATCTTTTTACTAGATGAAAACCAACTGAAAACCTTCCAAGAATCACCAGATGAAATGGTAAAAAGAATCCAAGCTCAACAACAGCAAAACGAACTGATAATTGCTAAAAACAAGCAACTAAGAGCTATCAAAAACAAACAAAAGCGAGCTGATTATCTAAAAAAGAAGTATGGTGAAAATTACTTAGATAAAACTAACTTAAAAGATGAAAACAACAATTAATATTGCCACCCCTACCCTAAAAAAATCTAGCAAAGAAGCTAACTTGATTGCTAGTATCTATGGGTTGTTATTTGTTTGTGGCGCGAAAGGGATCACTTTAAGAGAACTAATTAGGATCTTTAAAAAAGCAGGGATTGAAAAGGTGAAATTAGCACTCTTAGCACTTGAAAGGAAGTTAGCAGATGATGAGCAATCAGGAGTGGAGTTGAAAAAATTTGGTAATAGTTTTTCTTTGGTAACAAAACCAATTATCAAAGACTATCTCCACTTATTATTGGCTCATAAAGTCAAAAATCCCCTTAATTCCAAAGCAATGGAAGTGTTGGCTATCATTGCTTACAACCAACCTTGCACCAGACCCAGAATTAATGAAATTAGGGGAGTTGATTCTTTTCAAATTGTTGATGATCTAATAGCAAAGGAGTTAATTGTGGAGTTAGGGAGAACTGATAAACCAGGTCGACCTTTTATTTATGAAGTGTCAGCTAAGTTCTATGATTTATTTGGCATTGATAGCTTAGATCAACTCCCTAAGATTGAGCATTTTGATCTTGATAAATTTAAGCAAGGTAGCTTTTTTGATTCCAACCGCTATGGTGATGAATAACCTTATATAATTTACAACATGGATAAAATAGCTATTTTAACTTCGGGTGGTGATGCTAGTGGGATGAATGCCACCATCGCTTATCTAACCAAATATGCAATTGCAAAGCAATTGGAAGTTTTTTATGTAAAAAACGGTTATTATGGCTTGTATCACAACCATTTTATCACCAGTAAGGAACTTGATTTAACTGACTTTTTCTTTATGGGGGGAACAGTAATAGGATCAAGTCGTTTCAAACAGTTTCAAGATCCTAGCTTACGAAAACAAGCAGTTTTAAACCTCAAAAAACGTGGTATTAACAACCTTGTTGTTATTGGTGGGGATGGGAGTTATATGGGTGCTAAAGCACTCAGTGAATTAGGATTAAACTGCTTTTGTTTACCTGGTACGATCGACAATGATGTCAATTCCAGTGAATTTACCATTGGTTTTTGAACTGCTTTAGAAGCAATTCGGGTTAATGTTGAAGCAATTTATCACACCACCAAATCCCATAACCGCTTAGCAATCATAGAAGTGATGGGGCGTGATTGTAGTGATCTGACCATCTTTGGGGGGTTAGCTACTAATGCTAGTTTTGTTGTTACTAGCAAAAATAGCTTGGATCTCAATGGCTTTGAAAAAGCAGTGAGAAAGGTGTTGCAATTCCAGAACTATTGTGTTGTTTTGGTTAGTGAAAACATCTATGGTAAGAACGGTTTACCTAGTTTAAAAATGGTTAAAGAGCACTTTGAAAACAACGCAATTAAGTGTAACCTAGTTTCACTAGGACACACCCAAAGGGGCTTTAGTCCTAATAGTATCGAACTCTTTCAGATTAGTTTAATGGCTAAACACACGATTGATCTGGTTGTAAATAATGCCAACAGTCAAGTAATAGGGATGAAAAACAACCAAGCAGTTAACTATGATTTTAACACTGCTTTTAATTTACCAAAAGCTGATAGAACCAAGTTACTTAACCAAGTTAACACTGCAATTATTTAACGATGATTGACCATTTAAAAAGAACAAAGATAATCGCTACCTGTGGCCCAGCTTTAACAAAAAGCTTGGTTAGCTTAAAGATGCTTGATGATAATGAGTATGCAGCTATTAAAAAGGTTGCTTATGCCAACATTGAAGCAATTATTAAAAGTGGGGTTAGTGTGATTAGGCTTAACTTCTCTCATGGTACCCATGAAGAACAACAAGTGAGGATCAAGATAGTAAGGGATGTAGCGAAAGCAATGAACATCCCTGTTTCTATTATGTTAGATACAAATGGTCCTGAGATCAGGATAGTAGAAACTAAAAAAGAGGGTTTGAAAATCACCAAAGATAGTGAAGTGATTATCAACACCATGAGTAAAATGATCGCTAGTGACAACCAGTTTGCTGTCAGTGATGCTAGTGGCAAATACAACATGGTTAATGATGTGAATATAGGTCAGAAAATCCTTGTTGATGATGGTAAGTTAACCCTGGTTGTCACAAGGGTTGACAAACAACATAACCAGGTTATCTGTGTTGCAAAAAACGACCACACAGTTTTCACTAAAAAAAGACTTAACCTACCCAACGCACAGTACTCTATCCCTTTTCTCAGTGAAAAGGATCTGAAGGATATTGACTTTGGTTTAAGCCAAGGTATTGACTATATTGCTGCCTCTTTTGTTAATACTGTTGCAGATATTAAACAACTGAGAGATTATCTGAAATTAAAGAATGCTAGTGGGGTGAAGATCATCGCTAAGATTGAATCTAATCATGCTTTAAATAACATTGATAAGATCATTAAAGCTAGCGATGGGATTATGGTTGCTAGGGGTGATTTGGGCCTTGAAATCCCTTATTACCAAGTCCCTTACTGACAAAGGTACATGATTAAAGCTTGTCGCTTTTTTAACAAGCGTTCTATTACTGCAACCCAAATGCTTGATTCACTAGAAAAAAACATCCAACCAACCCGAGCTGAAGTGACTGATGTTTACTTTGCAGTTGATCGGGGTAATGATGCAACTATGTTAAGTGGGGAAACTGCTAGTGGGCTTTACCCTTTAAATGCAGTAGCGGTGATGCAAAAGATTGATAAACAATCAGAAACCTTCTTTGATTACCAGTATAACGTTAACTATTATTTGAAAAACTCCACGGCAAATAAAAGTAGGTTTTGACACAACGTTGTTTTACCTTTAACAAAAAAGACTGTTCCTAAAAGAAAACTTGTTAACAGTGCCTTTAAGTATGACTTTATTGTCTATCCTACTAATAACATTAACAGGATCTATGCATTATCAAACGCACGCTTAGCAGCAGCAGTTATTATTTTAACCAACAACAAACGGGTTTACACTGGCCATGGTGTTGATTATGGGATCTTCTGTTATTTAATTGATAAAAACCCCAACCAGCTAACCAAATCTGAACTGATTGAACTTGCTTGGAAAGCAATTAACCACTATCAAGCTTATGGTGATTTAGAAAAACTCAAACAGTGTTTAGCTGTCTATAATGAAACAATTATCAATCTTTAGATAAAAATTGGTGAGAGTTTGTAAAATATTGTACACGGTGCCTTAGCCAAGTGGACTCAAGGCCTGGAGCTGCAACCTCCATATCGTCAGTTCGAATCTGACAGGCACCTCCATGTACATGAAAGTTAACTACTCTCGATGTGCAAACGGGAAGTAGCTTAGTTTGGTAGAGCACTTGGTTTGGGACCAAGGGGTCGCAGGTTCAAATCCTGTCTTCCCGACCAAAAGGCTGGATACCTCAGTTGGTTAGAGGGCCCGGTTCATACCCGGGTTGTCGTGAGTTCGAGTCTCACTCCAGCCACCAAAGTTACTTAAATATTAAAGGATCTATAGCTCAATTGGTTAGAGCCCCCGACTCATAATCGGTCTGTTACAGGTTCAAGTCCTGTTAGATCCACCACTTTGCTGTGTTAGAATCATATTTGCCGCAATTTTGTGGAGACTTACCCAAGCGGCTGAAGGGTTCGGTCTTGAAAACCGAGAGGTGCTTTATAAGCACGCGAGGGTTCAAATCCCTCAGTCTCCGCCAAATAATATTTAATCGCGGGATAGAGCAGTTGGTCAGCTCGTCAGGCTCATAATCTGAAGGTCGAGGGTTCAAATCCCTCTCCCGCAACCATGGTTCCATGGTGTAGTGATAACATATCTCCCTGTCACGGAGGGGTTGCGGGTTTGATTCCCGTTGGAACCGCCATTGGTCTTGTAGCTCAGTCGGTAGAGCAACGGTCTGAAGAACCGTGTGTCGGCAGTTCGATTCTGCCCGAGACCACCATTAAAAGTTTTTAAAAAAGCCCTAAAAAGGGGCTTTTTTAGTGTTACCACCAATTAAAATATTTTTATAGCAAGCAGTTGCATATTTTTAATATAAGTTAGAATTATTGGTATAGTGTCTTCAGCTGTTTAATTCATATTAAAGCGCATGGAAAAAAATAGATCAGCTTTTCAACAAAACCAACAAGCATCAAACCAACCTTTTAACCAAGATCAAAACCAGTATTACCAAGATCCTAACCAACAACAATTTAACCAACCTGGTTTTGATCCAAATCAACAACAATTTAATCAACCAGGATTTGACCCTAACCAGCAATATTATCAAGATCCCAATCAACAACAATTTAATCAAGCTGGTTTTGATCAAAACCAACAGTATTACCAAGATCCCAATCAACAACAATTTAATCAATCAGGTTTTGATCCAAACCAACAATATTATCAAGATCCCAATCAACAACAATTTAATCAAGCTGGTTTTGATCAAAACCAACAGTATTACCAAGATCCCAATCAACAACAATTTAATCAATCAGGTTTTGATCAAAACCAGTACTATCAAGATCCTAATCAGCAACAATTTAACCAGCCTAGTTTTGATCTAAATAACCAACAATTTAACCAACCTGGATTCAACCAATCCCCAGCATTTGAAATCACACCTCAAGAGCAAAAAGCTGAACAGGAAATGTTTGGTGAAGAACCACCTCAAGTAGTTAGAGAGATCCATGAACTACCATTTGAAAAGATCCGTTCTTTTTTACAAAGTGATTTTGATAGCTATAACTTTCGCTTAAACTCACTTAAAAGCAAACTGGATAATGCCTTGTATTCACTTGATAAAACAATCCAAAACACCAATGAAAACACTGCTAATCTAGAAGCAATTAGACATAACTTAGAACAAAAGATTCAAAACCAAAGCAAGCAATTAAGAACTAACTTTGATACCCAAAAGCTTGATGATAAGATCAATGAATTGGAGATCAGAATGCAAAAACTAACCAGGAATTTTGAATCTCTAAGTGAACTTTCAAAGCACAACTCTTATCCTAATTACTATGAAAAATTGTTACCAAATGGTGGTGATAGTATGACCAATGTCTTTGAAAAAGCACTAATGATGAATTTATTGAGAACTACATTACCCCCTCAACCCCAAGTTCAATACTACCCTCAACCCTATCCATACATAAGACCTTACTATGATGAACCTATTTACGCTGGGTTTAGAAGAAGGGGTTACCGTGATGACTTCTATGAATAAAAAGCGTGTTTTAACTAATGAAACCATTTGATAAAAAACCTTCGCTGCAACCAATTTATGACATTGGTTTTGATGATGGTTATCTCCAAAGTGAGTATGAAAAAAATCGTTCTAAAACCGATGTTGATAAGATCGAAAACCAGCTTTTAAAAGAGATTAAAAGCCTGGAAGATGAACTTAAAAACCTTAAGGGCTTGAAGAATCAAGCAGAAGATAATCCTGAACTTGATAAAAAGATTAACCACTTGGAAGTTGATCTAAACCGTTTGGTTAATGAATATAAAAACTTCCAGTTCCAAAAGAACCACATGGTTGATAAGGTTAGTGAACTTGATAACTTAACCCGTTTTTATAAGAATGAACTAACCCGCTTACAACAAGAAAACGCTGATTTTCTCAACTCCAAGTATGCTAATTTAGCTAACTTCCAAGCTAACTACCACAATAAACTAAATGATTTTCACCGCTTAATAGAAAATCAAAACCAAACCATTAACCGCTTAAACCAAAAGATTAATGGTAACCAAAATCTGATTGATAATAACGTTGCTTTACTGCAAAACCCCAACATCACAGTTGAAAAAAAGAACTACTTACTAAATGTTATTGATCAACTTTACAATGAGCTTGATCAACTTGAGAATCAAAAAAGATTATTAAGTATTGAGTATGAAAATACCTATAGAGAGTTAGTTAGTGCAGATAATGAACTGCAAAATGTTTATGAAAACATCGATCAAAATCAGATCCAGTTTAAACACCAATACCAAACTTATAGAGATGAGTTAAGTCAACTTGAGCGCAAGATCCAGCTCACCAAACAAGAGTTAGTTGATAAAGAATCAGCACTAAGAGTAAAGATAGATGATGCTGATTTTTACATTAATGCCCGTTTAGCTGAACTTGATGATGTAGCTAAACAACTTAGCTTTCAAGATGGTATTACCAAGCAAAATGCTCAACATGTTGAGGATAAGTTAGTTGCTTTAAACAAAGAAAAAGACCGTTTAAATACCCAAAAAGAGGCCTTTTTTAACTTAAGACAATCTGCTTTAATTGATATCAATAAACTCCAGCAGGAAAATGAACTGTTTGCTAAGCACTTAGAACACCAGCAAAATGAGTTTGAACAAAAACAGTCTGATAGCCTCTTAAAGCTTGAAACTGAATATAAGGCTTTACAACACAAGATTAATGAATTTAAAAATGAAAGTGCCACTAAGAGCGAGGAACTTTTAAACCAAGAACGGGAACTATTTGAAAAACGCAGGGAAATTGACACGCTTTTAACCCAAGCATCTTTGGAATATGAACACCAACGTGAGTCAAGTCAACTACTCAAAGATAAGCAGAATGAAGTAAAACAACACTTCCAAAACTTAGAGTATGCTAAAAAGGAGCTAGACAAAGAAAGAAACCTCTTAGATCAACAAAAAAAAGTTGATAGTGAAGCAATCTTTCAACTCAAAGAAAAGGTTGCTCAAGAACGTAAGGAGCTTGAAGAACTATACCTTGTTAAAAAACAAAAACAAGATCAGAAGGAAAATGAACTGTTGTTTTTTGAAAAGCAGTTAAAACAACACCAAGCAGATTTTGAAAATGAATTGGAAGCTAAACAACAGGAGTTGTTTGAAGCTAAACATGCATTGGAACGTTCCTTTATCAAACTTGAAGATAAAGAAAAAGATCTTAACACTAAAGCACAACAGATTGCCAATGAGTTTTCCCAACTAAAAACTGATAAGTCAAAGAGTGCTGATTTTGAACTAATGTTGCAAAATGAGTATGAAAACTTGCAACAAGAAAAACAAAAGTTATTCCAAGAACGTACTTACTTTGAAAGGAATGCTGCGGTTTTATCAAACCGGTTACAACAAAAACGTGAGGAGTTATTACAACAAAAAGAAACGCTTGATCAGCTTACAAAAAGCTTTGAGCAAGAACGGTTAATCAACCAAAGGGAACACAAGGAGTTGGTTGCATCAGTTGAAAAACAAAAGGAGATATTGGGCAAAAAACTCCAAGATTTTTCCCAAACTTCACTGAACGCTTCTAAAAATTTAGCTGAACGGGAGATGGCAATCAAGTTTAAAGAAAAGGAGATAGAAGCAACTGAAAAGCAACTGTTAAATGATGTTAATAATGCTGAAGTTATCCAAGCAGACTTAGCACAACTCAACCAATCACTTAACCAAGAACGCAGTGAATTGCAAAACGCCAAACAAAGGATTGCTGATTTTCACAATGATTCACTAAAAAAACTCAATGAGTATGAACTTAGCTTACAAAAACGGTTGCAAGAATTACAAACCCTTGAGGCTAACCAAAAACAACATTCGTATCAAAATCAAGCTTACTTTGAAGGTGAACTTGATAAACTTAACAGAGAAAAACAAGCTTTTTTGAACTTACGTAAGAAACAAACTATGGAGGTTGATGCTATTAAACAAAGGTTGAGTGATAAACATCAAGCTTTAAATATGCAACAAGCAGAGCTAGATAGAAAAACCCATGAGTTAAATAATGCTTTTTTAAACCATGATGCGGATCAAAAGAGTCTACAGGACCAACTAGCAACTGTTAAAGAGACCCAAAAACTAATTGATTTAGAACGTAGTGCACTGCTTGAAAAGCAACGTGAGTTTGCTGAAAATGTTGCTGGTTTTAAGCGCCATTGGTCTAATAAAACTAGTCAACTCCAAAAGGTTTATGAACTGACCAAAAAACAGGAAAGTGAGCAAACCCAAAAGGAAACAGAACTAAAGATTGCTTTTAGTGATCTACAAAAAGACTATCAGGTTTTTGAACTCCAAAAGGACCAAGAATTTAGACAAATTGAAGCTAAGCAACGTGAACTTGACAAGTTAGCTGAAAAAAATAATCAGGTCAAACTAGAACTTGATAACAGGTTTCAAGCGCTGCAAAACCAAAAGCAAGACACAGTACAAGCTCAGCTAGAACTGGAACGTGAACAACACCAGTTAAACCTTGAGCAAACTGCTTTCAACCAAGCTAATGAATCACTTTTAAAACAACGTGAACAACTCACCAAAAAGATCCAAGCTTTCCACTATGAGTTGAAAAAGCGTAACCAATTCTTAGCTTTAAAAGGGAAAAGGTTGTTTGCAAAAGAACAAGATCAACAACGCAAAGATCAGGAGATCAACTGACGCTTTAAACAGTTTGAAAAGGAATATACTGATTTTGATGAAGCTAAGAAAAGGGAACTTGAAGAACTTGAAAAGATCAGAAGAAGTTTAAGTCAAAGTAACGTTGAATTAGAGAGAAAAAGAGAAAAACTGGCTACTGATTTCACTAATTTAAATAAGGTTCAACACAACACCCAAATTAACCGTGATCAACTTAACAGTCAGATCAGACAGTTCTTATTAGAACGCAAAAACTTCCAACGCTTTAGTAATGAAGCTAATGCTAAAAAAGCCTTTTTAATTAAGCGCTTAAGAAGCTTTGCATCCAATCTAAAACTCCAAAAAGAAGCGTTAGCAATCCAAAAACTAGAGTTTGATAAGCGTGATGAACAACAGAAAAAAGAGTTACAGCAAGCTACTTTACAACTAGAACAGTTCAAGTTTGAAAAGCAAAACTTTGACATTGAAAAACAACGCCAACTAGTTGCTATTAAAACTCAGTGTGAAAAACTTAGTGATGAAAAAAAGGCACTAAACCAAAAGCTAGTTGAACTAAAAAACTTATCCCAAACCTATCTTGCTAATAAGAATAAGGCTGAATACTCCCAGCAACAACTCCAACAGAAATACACCAATTTACTTGATCTGAAGGAAAACTTAGAGAGAACCAAAGATCAATTAGATAAAAAACATCGTTCTATCTTCGCTAGATTAACTAAGTTTGCAAATGACTTACGTTTTGAAAAAAAGCAACTGTTAAAAGCACAGCGCATAGTTGATGATAAAAACCGTCTTTTGAAAGAAAATGAACGTAACCTCCATTTCCTTTCCAATGAAACAGAACGAAAACGAGCAGTTCTCGAAGATCAAATTTCTTACTTTGAAAAACAACGTAAACAAGCTACTGATGCGATCCTAGCATCACATAAAGAAGTTAAAAAGAAGGAAGGTGAACTGCAAAAGTTACTGGTTGAATTAGAAACAAGAAAAACCAAACTCAACAATGATTTTGCAAAATTCTCAAGACAACGTGAAGAGTTTGAAAACCAACGCTTAAAGCTCTTGGAACTGCAAAAAACCCTGCAAACCCAAACTAATTCCAACAACTTTAAAACCAAAGCAATCCAAGAGATTGAAAACAGTTATAAAAGGGGGATGGAAGAACTTAACTTCCAAAAGAAGGAGTTTGATAAGAATAAATCACGCTTATATGAATACTTTAGAAAGATGCGTGATGAGATTGAAAGAAAGGAAAGTCAGGTTAAGTTAGTTTTAAAAGAGACCCAAAGGAAAGCCAACCTCTTAGAAGCACAAGCCAACAAACTTAACATTGAAAAAAACACTATTGACTTTAAAGAAAAAGAGTTAAAAGCCTTTAAAGATAAGGTTGATCAAGACATTGATTCAACCAATAAACAACGCAAGGAGTTAAATGAGCTTTTAAATGAAAACAAGTTATTACAACAATCACTAATCGAAAGAGAAAGGGCTATTAATTCCAAAGATTCACTTCTAAATAAGAAGATAGAAACGATTAAACGCCAACTCCATGATAAGGAGATGCGGGTGTTGCGCTTGGTTGATAGAATGAAATTAGCAGAACAGAAATACCAAACAGAAATCAACCGCTTAAGAACCCAAACTTTCGATTCTGAAAAACAAGACATTAAAAACTTCTTCCCACCGTTATTTAAAATTAACGGTAATGATATGGCCTTTCCTTACTTATACCCCTGACTATATCCTCAACAAAAGCAAGATGATAACACTCTGCAAATTCGTCAGCTTTTTGAACAGCAGCTGCAGTTCATGCAACAACGCTATGAAAATGAGTTAAATGAATTGCGTAGACAACGTAATTTACTTGAGAAAAAACTTGATCAAATCCAACTAGAATCCCAGCTTAACAATAAGCAAAGTGAGTTTAGTAAGGTGGAATCAATGATGGAAAAACTACTTGAAAAAACTGAGAGTAGGTTAAATGATTTTGATCAGAAAATTAACTATCTCACCAAGAAAGTTAACCAACACAACACCTATCAACCAAGTTCCTATCAACCAACTCCTTCTTATCAAGACAGTGATAAACAACAGTTGTTATTTAGAATCCAAGAACTGGAAAAACAAAACTTATTCCAACAACAATTTCAACCTGCACCAGCTGTTGTCCAACAACCTACTAGTTTTGCAGCCCCTAACATCACCAAACAACAGCAAATTGCCCAACTTAATGCTGAAATTAACAACATTAAAAGGTTGATTGCCCAAAAAGCAGCAAGTAAATAAAGATGGTTAATAATGAATATCAACAACTAAACACTTTAGTTGAGAGTGATGATGAAGCGGATCTTGTGATTGCTAACCTAGTTAAACAACTCAATGAACTAAAGCAAATCCTTGTTTCACTAGATAATCAAGAAGCAAGTGCCACTGCAGTTACTGATAAAAAGGAAGAGGAATACAACCAAAACCAATCCAGTTTCCATAACTTCAGCAAAGAAACACTGCAAAAGCAAGCAAAACGTGGTTTTCTTTTACTGGAACGCTGTTCGTTGGTTGGGTTACAACAACTAGAGTTGGAGTATGTTAATTTGTTGGGCAGAAGTTTTGATTCTTATCAACAAAAAACAGAGCTTTTAAACAACTTAAAGGAGCTTGTTGATGAACATTTCAGTGATACTGAAAAAATTATCAATACCCTTGAAAAGATCTTTGATGTTATTGGCGGTAGTGAATATACCCCTGTCTTAAACTCGTTTTTTAACAAGCTTTTAAGTGATCCTGATCCAATCCAACGGGAAATTGGCTTAAGACAATTTATCATCACTCTCCGTCAGCGCTTTAAAAAGTTATCACAAAAGATTGACAGTTCTCTCAAACAGATAGAAACAGAGGCTAAAATAGCCACTGAACAGGTTCAAAATAGTGAAGTGATGTTCGGTCCCCCTGATATTGCTAATGATCATGAGTTAAACCTGAACTGACCTGATAGTGAAACAGATGCTATCTTAAGTTCAATGGAAAATGAATTGGAAGCTGCTTTATTAGCAAAACACCAAGAAGAACCACCGTTAATTGTTACCCCACCCAGCTTAATAAAACCAACTGTTAGTCAACCTGAAGTTGAAGTTGTTACACCTACTAACAACACTAATTTCCAACCCCAAGTTGATCTCAAACCTACTGATTTGAAAAAACAACAGAAGAAAAAACCACTTAACTTTATTACCCGTCCTGTTTTCAAAAGTAATTTGCCACCGAAACTAAGTAAGGATGACATAGTTCATTATGCGCACCAGTTACTTGAAAAAAATACCCATAATGAATAGTGATAGTGATCTAAAACTCCAAAAGGTGTGGATCGAGCGGCATGTTGATCAAGATGAACTTAGTTTAACAACTACTGCAGTTGAACTTAAAAAGAGTGATGAACAAAAACCTGTTGCCATTAAAAGTAGTGACTTTATTGGCCATGAAGAATTAATCTCTGTTCCAGTTTTACTAATCCCAACCCCTGTTGTTAAAGAGATTGACCAACCAGTAGTTATTACTCCAGTTAAAGCAAAACCAAAAGCAACTAAAAAGAAAACTCCTGTTAAATCAAAACCAACTAATAAATCAACTAAACAAACAAAACCTAAACAACCCAAACCCAAATCAAAACAAGTTCAAAAAACCAAAGCTAAACCAACCCCAAATTCAAACAAAAAAAGCATAAAAAAACCAGATCTTAATCTGGTTTTTTTAGTGTTAACAACAGTAGTTTTAACTAAAAACTGGAAAGGAATAGGAAGAACTACCTGGGGTTAATCCTAGTTTAAACCGTGCGGTGTTAAAATAATTGCTTTTAGAGCGATAACTATTATTTTTATTCTCATTATCATCATCAGAACCATTGGCATTAGCTGTGGCACTTTGAAAAATGTCATTTACTGCATGTGCAACCCCAGTAAGTGCATTAACTATCATAATAAGTCCTGCACTAAAAGTTCAGAAAGATAATCCACCAACAACTTGCTTAGCTTGTGCTTTTTCTAATTTATACATATAACTCCTTTTGTCTATTAATCACATCTAAATCTAAAAGTGCCAAAGTTTTATAATTGATCAACTGTCATCATAGCTCAATAGGACAGAGTATCAGCTTGCGGAGCTGAGGGTTACAGGTTCGATTCCTGTTGGTGACGCCATTAACTTTATTTGCCTATCAGTTAAATAACTGGTAGGCTTTTTTATTGTTTTGTAGTTTATCAAGGGTTAATTTAAGTTGTAGTCATTTCATTTTGGACAAAAAGAAATTTTTATGCTAAGATAAAAGTGTTTAAAAGTGTTGCAAAGTGTGACAAAGTGGAAAAAATGCTGCTAGGTACCTTTAATCTTACCCTTGATAACAAGAACAGAATTAGCTTGCCAGCTAAGCTCCGTAGTTTCTTTGATAGCAGCATAGTTATTAATCGCGGCTTTGAAAACTGTTTGGAAATTAGAAAACCTGCAGACTTTGAGAGTTATTTTCAAACCTTTAATAACTTCCCTAACACCCAAAAAGACACAAGAACATTAAAACGCTTAATCTTTGCTAATGCTAATCTAGTTGAACTTGATAGTGCAAACAGAATCCTAATCCCTAATAACTTAATTAGTGATGCTAAGTTAGATAAAGAGATCGTGTTAATTGGTCAATTTGATCACCTTGAAGTTTGGGATAAAGTGCAATATGAACAATATCTAGCTAGTTCAGAATCACTAGAGACAGTAGCTGAAAGGATGAAAGATGCTAAATAACCAACAGATTCACCAGAGTGTACTGATCAATGAAGTGATCTATTACCTTAATATTAACCCTTGTGGTAACTATTTAGATCTAACTGCAGGGTTTGCAGGACACAGTCAAAAGATCTTAGAAAAACTAACAACGGGAACTTTAACAATTAATGATGTTGATAAAGAAAGTATTAATTTTTGCCAAAAGCTTTTTTTCAAAAACAACAATGTTGTTATTATTCACGATAACTTTGCTAACTTCCCAGTCCATCTTAAACAATTATCAATAACCAAGTTTGATGGGATCTTAATGGACCTTGGTGTATCAAGCCACCAACTCAACCAACCTAATCGAGGTTTTAGTTTTAAGAATGATGGACCGATTGACATGCGCATGGACCAATCCAATCAGAAAAATACCGCACTAATAGTTCTAAAAAACTTAACTGAACAAAAGTTAAGTCTAATCCTTAAAAAGTATGGTGATATTAAACACCCTAAACCAATTGCTATTGGACTGAAAAAAGCAGTTCAAACTGAAAAAAATCTTACCACAACTCAACTAGCAAAAGTGGTAAAAGAATGTGCTACTGGATTTGAAAAATACCAATCAAGAAACTATCTTGCTAAAGTTTTTCAAGCAATTAGGATCTATCTTAATGATGAGATTACTAATCTGAAAACTGCGTTAACTTTTATCCCTAATCTTTTAAAAAACAACAGCAGGTTTCTTGTGATTGTTTTTCACTCCATTGAAGAAAAAGTTGTAAGGAATTTCATTGCAAAACTAACCAGTTTTATCCAACCTGAAGCTCTACCCATTAAACTCACTCCTGCTTACCAGTTAATTACAAAAAAACCAATCCTACCTTCCCAAAAAGAACTTGAATTAAACCCGCGTTCGCGTAGTGCCAAACTCTTTGTTATCCAAAAAAACTAGTATGTACAAACCAAAAAATATTAACAGCGTATTAACCTTTTATAAGGATCAGATCCAACTGGTTGTTAGTGATGATCAAAACCAGTTCAACATCTTGTTTTACCAAACAATTGATAACGATGGCTTTTATTCAAAACAACAGTTGAAAAACAAACTAAGACTCAAGTTAGCATTAAACCAACTAGTTGATCAAGCTAACTATTTTCTTGGTTTTAAACTGGAAAAGGTAGTTGTTGTTCTCGCTGAACTGATTGATGATTTGAAGATCCATAATTTCAAGAGTGAGATCTTTTTTACTGGTTATGATTTTGATCATAAAGCGATGATTAAAAAAGAAAAACAACGCTTTTGTGAGCAAAATAACCAACTAACAGTTATGGATACAATGGTTTTAAACTACCATGATGTTATTAACAATAAGATCACCAAAAGCTTTGCATTTAACAAGAGCTATGTAGCTAATTTAGTGGCATATTCCTCTAAAAGTAACCTGATCGGGGAGTTGAAGTTCTTTTTAAAAAGAAACGTTAATCTTAAGGTTAAGAAAATTATTAGTCACCACTTAGCATTAGCCAACTCCTTAAGTAAGAAACAAAACAACATGTTTGTTTATTTAGGACAAAAAACTACTGAACTGATGCTATTTATGGACAATGCTTTAGTTGATGTTATTACCAACCAGTTTGGTAAAAACCACTTTATTGATATTCCAGCTAACCAGGAAAACAAACCACTGCTTGAGTTTTTAGTTGATAACACCACTAAGATTGGTGATTGTTATTCGCTTGGTATGACCTATACAGATGGTGATAGTTACAAAGAGATTAAGGCTTTGACTATTGGTGATTTAATGCAAACAGTTAGTGACAAGATCAAAACCTTAATTGATTTTATTAACAGTGGTTCTCTAACTTTTTTCAACAAGTTTAAAACCTTACCTAAGCTATTGTATTTTTATACAAGATCAAAACAAATTACCAACCTTTTTCAAGCTAATGTTGCACTTATCAATCCCCAGTTTAAAACTGTTGATATTTATAAGAACAAGATCCAGTTTATTAGTGAAAACTACCTGTTAAGCTGTGAAGCGATTAGCTTGCAGATTACCAATAGAATCAAAAACCAAATTAGTTTTGATTTCACAAATGCTGATAATATTCAAAAACCTAAACCAAAAAACACTTCATGATCTTATCAAAACACCTAACAAAGTTTGTCCAACGCTTGGTTAAATAACTATGGATGAAAATGAAACTCAATTCAACAAGTTAAACCAAGTTAAAAACAAGCTGAAAATTGGTGTTTTTGGGATTGGAGGTGCTGGTAATAACATTGTTGATGCATCACTTTATCACTATCCTAATTTAGCAAGTGAAAACATCCACTTTTATGCTATAAATTCAGATTTACAACACCTTGCATTTAAAACGAATGTTAAAAATAAACTCTTAATTCAAGACCATACTAACAAGGGCTTTGGAGCGGGGGGTGATCCAGCTAAAGGAGCTAGTTTAGCAATAAGCTTTCAAGAACAGTTTAATACACTTACAGATGGGTATGATTTTTGTATCTTAGTTGCTGGATTTGGTAAGGGTACTGGTACAGGTGCTACCCCAGTTTTTAGCAAGATCTTAAAAACTAAGAAGATCTTAAATGTTGCTATTGTTACCTATCCATCTTTAAACGAGGGATTAACAGTGAGAAACAAAGCCACTAAGGGGCTTGAAATTCTCAACAAAGCAACTGATAGTTACATGCTATTTTGTAATGAAAAATGTACAAATGGTATCTACCAACTAGCAAACACAGAGATAGTCAGTGCCATTAAAAACCTAATAGAACTAATTACTATTCCTTTGCAGCAAAACATTGATTTTGAAGATGTACGTGCCTTTTTTCAAACCAAAAAAACTAACCAAGATCAACAGCTTTTTACTGTTACTCACCCCTTTAGTTTTAGCTTTGATAGTAAAGATAGTATAGAACAGTTTGCTAAACAGTTTAAGAACTTTGAAAAAGTTAGTTATTTTGACCACTCTATAGTAGGAGCTAAAAAAGTGTTATTGAAAGCTAACATTAACCAAAAGATAGTCAAGCTTAACTTCAAGCAGATCCAAGATATTATCTGAACTAAAATTGACAACTACCAACTTGAGATTAGGTTAGGGGTTGATTTTGTGACAACCATCCCTAATATCCAAATTTTTATCCTCAGTGAACACAAAAATCCAGTTTCGCTTCCCATTGATAATAAATCAACTGAAAACAACCAAAATAAGTTGAAACTTTTAGATGAGCTGAAAGAACTTGGCATGAAATATGTTAAGCACCAAAACCAAATCTACTAATTAATTTAATTTATCGTTTAGAATTGCTATCTTAAGCAATAGTTTATGGGTCAAATCAATCGGAAGTTTAGCGAAAAGCAGTTCTTACTTTTTGTTGTTAACTATATTGCTGGATTTGGCTTTATTGCTACTGCTATCTCACTGTTTCGCTTAGGACCTTTTTCTTGGTTAATCTTTCTGCTTGTTAGCTTAGTGAGTTTAATTGTTACCTTATCATTCGCACGGCTTTCATCAATAGATAGTCAAAACTATGGTGGGCCTTATCTTTGGGCTAAGAAAGCGGTTGATAAAGAGAAGATAGCAGGGAGAATGTTTAGCTTTTTTACTGGGTGAAATAACTTTATCATTGGTCCCCTTTCAGCAGCAACTGCACCACTTTTTATCCTCAATTCCTTTAGTGGTATTGATGGGATTAGAGGTAACTTAGTTAACACTTGAATCCTAATTGCAATAGGTTTTTCTTTTTATGTATTACTAGCATTTATCTCAACCAAAGGAACCTCACTAAACAAGAAACTAATAGCACTATTTGCTTCAGTGAAGTGGATTGTGATCCTCTCAGCACTAATAGTAGCAATCTATGTTATTGCTAGAGATGGTAATGGTTATAGTCAAAACAATAACTTAGAAAGTGGTTTTTTTGGGAGAAGAGAGATTAGTTTTGCACAGATAGCAACGGTATTTATTACCTTCTTTTATTCTTATGCAGGGGTTGAAGATATCTCAGTGATGACTCCTGATGTTAAAACTAATAACTTTAGAAAGATATTAATTGTCTCTTTTATAGCAGTTTTCCTCTTTTATTTCATTGGGATTATTATTCTAAATGGTTTGCAAAACATTGCTCAAAGAGGTGGGGAAGCCAATTCAATTGGTAATGTAGCGGATATCTTTAAAAAAGCTGCTGGGCTTGGGACTTTAATCTTTTATGGAGTTGGAGCATTGTTTAACAATGTCTCAACCAGACTTTCAACTATTATTGCCAACTCCAGAAAGATTCTTCCGCTTGCTTATGATAACTATTTACCTAGTTTCTTTTACAAGCAAAACAAAAAAGGTGAGTTTCAGAATGCAATTTGGTTTACCTTTGGTACTACTTTAATTGCAATGACTTTGCTTGTCTTTATCCCTTTAGTTGCTTCTAACTTTGATTTTGATAATGCTACTGAGTATGCAGCATCTGTTGGCTCAGCTGCAACTTTGCTACAATATATTTTTGTTTTTTTATAATCTTTAAGTTTATCTATAAAAAAGAACCACTCTACCAGAAAAAATGGGTTAAAACAACTGAAGAATTATTGTTTTGTTTAGGAACAATTGTCATTGTTTTAATGTTGTTGGTTTATCTGTTTCCTGTTATAGATGGATTTTCAAAATGGGAAACTAAACACACATTAACAATTGTGTTATATGGGGTTTTAAGCCTGATAGGATTGGTACTTTTTTTGCTCCAAGAATACAAACATAAAAACAAGCAAAATGCAAACAAACAAACAACCCAAACAACAGTTTAGTGAAAAGCAATTCATTGCTTTTGTCTTTAACTATATTGCTGGATTTGGCTTTATATCAGTGGTGATGACCATGTTTGATGTTGGGCCATTTTCCTATCTTGTTTTAGGGTTAACTTCGTTTGCTATTTTAGGAGTTGTGCTTTCTTTTTCTCGCCTTTCAGTTCTCTGTGGTAATAGTGCTTATGGAGGGAGTTATTTAATTGCTAAAAAAGCAGTTGGTACTAACAGTAAAACAAAAAGGTTTTTGTTTTTTAAGTGGGTGGAATGTATCGTTAACAGGATCTTTTAACGGTGTTGTTATTCCAGCAGTATTAATCTTTTCCTTTGCAGATATTCCAGTAGTTAAAGCGAATAATAACATCATTATTGGTCTTTTAGTAGGTGGATTTTTGTTGTTTGGCTTACTTACTTTTATCTCGTTATTTGGTTTAAAAATTAACAAGAAAGCAATCTTTTATTTTGCTGTTATTAAGTGGATAGTAGTAATAGGTGGGTTTATCTTAGGGATCTATTTAATTGGTACTACCAATGGTAAAGGGTTTGTTGAAAACAATTTAATTGGGACTAGGGAAAACATTGATTTTTTCAAGATTATCTTTATTAGTCTGGCTTTAACTATTGCTTTTGCAGGGACAGAGGATTTAGCTTCGATTACTCCTGATGTCAAGTCAAATAACTTAAGAAAGTGTTTTTTAATTGCCTTTGGGTGTGTTGTGTTACTTTACCTAGTTGGGTTTGTTATTATCAGTGGACTTGATGGGATTAGAGGTTATGGATTAGCATTAGGTAATAAAGATCCCAAGGCAATTAATAACTATGGATCTATCTACCGTTTGGTAGGAGGAGTTCCTTTACTTGTTATCTATGGACTTGGGTTACTTGTCAATTCCTTAGCATCACGCTTATCAATGACAATTACAACAGCTAGAAAATATGTAGCTTTAGCTCAAGATGGGTTTTTACCCTCTTTTTTAGCCAAAACTAATAAACATAATGAGTATCACCATGCAGTTTTAATTAGTAATCTAATGACTTTATTAGTGATGCTAATTATGGTAATAATCCCCTTTTTACCAGACCATAACAACAATAACAATAGTTTGTTTAATGCTATTGAACAGTTGGTTACAGTTACCATTGAAATGGCTGCAGCCATTTCTTTGATCCAATACTTTATTACCTTTATCTTCTTTTTTATGATCTTCGCTAAAAAGGAAAACCAGAAGTTAATTCCCTTGTGAGAAAAGGTTAGTTATGTAATCAGCTTTGCTTTGGTAAGTGTGTTGTTGTTTGTACCACTTTTCCCTTTTAATCAGTGAACAGTGTTTAACACCTTTAAGATAGTTGTTCTAATTTGTTTTTATCTACTTGGTGTTGGTTTTTTTGGTTATGCTGAATGGAAAAATAAAAACAAATACCAATTAATGAATAGTAATAGCTAATCTACAGTTCATTAAGCGAAATTGCTTTTGAATCGGCAATGGTAAACCCTTCTCCAACTCCCCCTCCCCTTCTTCTTCTTCTTCTTCTTCTACCCCACTCCCCACTTTTTCTAACATCAATGTTGGGGTTAAATCAATGATCACTCAACATTTAAATAAAGAAAACACGCGGTGGGTGTTTACCCCTAACTCTTCACCTGACATTTGAACGGGAGCAGGGTATCGCAAGGGTAATGACAACAATAACGGTATCTCCTTTGAAATGGTGAAGCCTAGCAATAGTAGTACCCCCTTTAATCCCAATTCTTCTGAAAACCAAGTCACACCATCAGGTAGCTCCCCAGCCAAAAAAACAACGTATGATGCTTTACCAAACTCCATCAGTCCCACCAGTGACTGTTAAAATGTTTATTAAAAAAACAAAATAAAAAGCGGTTTTATACAATGTATAACCTGTCTAAAAGACAATTTCATGAAACAGTATTTAGATTTAGCTAGTTATGTTTTAGCAAATGGTAAAAAAAGAAAAAACCGTACAGATACAGATACTTTAAGTGTCTTTGGTTACCAGATGAAATTTGACCTTACTAATAGTTTTCCTTTATTGACAACTAAAAAGGTTAATTGGAAGGCAATTGTCCATGAATTGTTGTGATTTATTAAGGGTGATACCAACATTAAGTACTTAGTTGATAATGGGGTGAACATCTGAAATGAATGACCATATGAAAACTTTAAAAAATCACCAAGTTTTCAAAACGAAACACTCCAAGAATTTATCTTAAAGGTTAAAACTGATAATGAGTTTGCTAAACAATTTGCTGATTTGGGTCCTGTTTATGGCAAGCAATGACGTAATTTTAATGGTGTTGATCAACTCAAAAAAGTCATCCAAGAGATTAAAGAAAATCCCAACTCAAGAAGGCTAATTGTCTCAAGCTGAAACCCTAGTGAATTGGAAAAAATGGCATTGGCTCCTTGTCATTCACTCTTTCAGTTCTATGTTGAAGAAGATAAACTAAGCTTACAGCTTTACCAGCGCAGCGGTGATATCTTTCTTGGTGTCCCATTTAACATTGCATCTTACGCCTTACTTGTGTATTTAGTTGCTCATGAAACTAAGTTAAAACCTGGTTATTTTATCCATACACTAGGAGATGCACATATCTATGAAAACCACATTGAACAAATTAAATTACAACTAACAAGAACAACCCTAGACCCCCCTCAAGTGGTTTTGAAAAGTGATAAATCAATCTTTGCTTATAGTTTTGATGATATTGAGTTAGTTGGTTATAATTACCATCCATTTATCTATGGGAGGGTTGCAGTTTAATGCTAATTGCTATCTGAGCGATGACACAAGAAGGACTAATAGGTAATAACAACACTTTACCTTGGATGATTAAACAAGAGCTAGCTCACTTTAAAAAACTACGTTATTTCAAGCTTTGTTAATGGGGAGAAAAACTTACGAATCACTCCCCAAGGTATTTGAAAAAAGAACAATATTTCTCCTTTCAAAAGATCAAAACTACCGTTTTGAAGAAAAGGGAAGTGAAGTGAAAGTTATTAATGATTTTTGACCACTAATTAAAAGTTACCAAGCAAATAAAGAAAAGGATTTGTTTATTTGTGGTGGAAAAAGTGTGTATGAACAGACCATTAATGAATGTGATCAGTTAATTGTTTCAATCATTAAAAAGAAGTATAAGGGTGATCAGTTTTTGAAGGTTGATCTCAGTAAATTTGTACTTAATGAAGTTGTAGAGTTTGAGGAATTTAATGTTAATTATTATAGAAAGAAACAACAATAAGAGATATGGCAGCTAACAATAAAAAGTACTTTTTAGAATCATTTTCCCCACTTGGGTATGTAAAGAATAATTTTCAGGGCAACTTACGTTCTGTAAACTGGAATTTGGTTGATGATGAGAAGGATTTGGAAGTGTGAAACAGGATTGTTCAGAACTTTTGGTTACCTGAAAAGATCCCTGTATCCAATGACATCCCCTCATGAAAGAAACTCTCAAAGGATTGACAGGATCTGATCACTAAGACCTTTACTGGTTTAACACTACTTGATACTATCCAAGCTACCATTGGTGACATCTGTCAAATTGATCATGCTCTAACTGATCATGAGCAGGTTATTTATGCAAACTTTGCTTTTATGGTAGGGGTACATGCCCGTTCCTATGGAACGATCTTCTCAACTTTATGTACATCAGAACAGATTAACGCTGCTCATGAGTGGGTTGTAAACACTGAAAGTCTCCAGAAAAGAGCAAAGGCATTAATCCCTTACTATACGGGCAATGACCCGTTAAAATCAAAGGTAGCAGCAGCTTTAATGCCTGGGTTTTTACTGTATGGTGGGTTTTATTTGCCTTTTTACTTGTCATCAAGAAAACAACTACCAAATACATCTGATATTATCCGCTTAATCCTTCGTGATAAAGTGATCCATAACTATTACAGTGGTTATAAATACCAACGTAAACTAGAAAAACTCCCTTTAGCAAAACAAAAGGAGATGAAAGCATTTGTTTTTGAACTAATGTATCGGTTAATTGAACTTGAAAAGGACTATTTAAAAGAGCTTTATGAAGGGTTTGGAATTGTTGATGATGCCATTAAGTTCAGTGTTTACAATGCTGGTAAGTTTTTACAGAACTTAGGTTATGACTCCCCGTTTACTGCAGCAGAAACCAGGATTAAACCAGAGATTTTTGCCCAACTATCAGCACGTGCTGATGAAAACCATGACTTTTTCTCAGGAAACGGTTCGTCGTATGTGATGGGAGTTAGTGAAGAGACAAATGATGATGATTGGAACTTTTAAGTTATGCATAAAGATATCAAACTAGTTAAGGAAACTGAAATTAGAAAACCAATTGGTTCTCCTTTTATTGTCTATTTTTCATCTATCTCCAACAACACCCACCGTTTTATTGAAAAACTGGGTTTTCAACACAAAAGAATCCCAGTTGATATAACCCAAAGCATTACTATAAGTAATGAGTATGTTTTAATCTGTCCAACTTACAGTGGTGGGGGTAACCAGGTTGAAGGAGCGGTACCCAAGCAAGTTATCCAGTTTTTAAATAACAAGCATAACAGGGAGTTATGCAGAGGAGTTATTGCATCTGGTAACACTAATTTTGGAGATACTTTTTGTCTTGCAGGAACTGTTATTTCCAAAAAACTAAACGTCCCTTTGTTATATCAGTTTGAACTTTTGGGAACAAAAAATGATGTAGAACAAACTCAAAAAATAATTGCCAATTTCTTTCAAAACAGCAACTAGTATTTATAGTTATCCACTATGACATCCAAAGAAAAAATCCCTACTTTTAATACTGAAGAAGATGTTGAAAGTTACATTTCTTTTAATGCCCAAGCCAAAATCTATGATGATTTTGCAATCGATTTACAAGCAGTTGAAAGCTATATTCAAGAGCATGTAAAACCCAAAACCAAGGTCTTTCATTCCACCAAAGAACGCCTTGATTTTCTGATTAAGAACGATTATTATGATGAGAAGATCATCAACATGTACAGTTTTGAACAGTTTGAAGAGATCACCCATAAAGCATATTCATACCGCTTTCGTTATGCTAACTTCATGGGAGCATTTAAGTTCTATAATGCCTATGCTTTAAAGACATTTGATGGTAAGTACTACTTGGAAAACTATGAGGATAGGGTGGTGATGAATGTATTGATGTTAGCTAATGGCAACTTCAATAAGGCATTAAAACTCTTAAAACAGATTATCCTTAACCGTTTTCAACCAGCAACCCCTACCTTTCTTAATGCTGGTAGAAAGAAACGTGGTGAATTTGTTTCATGTTACCTGTTAAGGATTGAAGATAACATGGAATCAATAGGTAGAGCGATAACAACTACACTACAACTATCAAAACGTGATGGGGGAGTAGCACTTTTGCTTTCCAACTTACGTGAAGCGGGAGCGCCCATCAAAAAGATAGAAAACCAATCATCAGGGATTATCCCAATTATGAAATTGTTAGAGGACTCTTTTTCCTATTCCAACCAACTTGGACAAAGACAAGGAGCGGGAGCGGTGTATCTCCATTGTCACCATCCTGATGTTATGCAGTTTTTAGATACTAAAAGGGAAAATGCTGATGAGAAGATCAGAATTAAATCACTCTCCTTAGGACTTGTGATTCCAGATATCACCTTCCAATTAGCAAAAAATAACGAGATGATGGCACTTTTCAGTCCATATGATATCTATCAGGAGTATGGTAAGGCTTTATCTGATATCTCAGTAACTGAGATGTATTATGAATTGCTTGAAAACCAACGCATTAAAAAGACCTTTATTAGTGCTAGAAAGTTCTTTCAAACAATTGCTGAACTCCACTTTGAAAGTGGTTATCCCTACATCTTGTTTGATGATACAGTTAACAGGAGAAATGCCCACAAAAACAGGATAGTAATGTCTAACCTTTGCAGTGAAATTGTCCAACCATCTTTACCTTCTGAATTCTATTCAGACCTTACTTTTAAAAAGGTAGGTAGTGATATTAGCTGTAACTTGGGGAGTTTAAATATTGCTAGAGCAATGGAAAGTGGTAGTGAGTTAGCTGAATTGATTCAACTAGCAATTGAATCACTGGATTTAGTGTCAAGGATCAGTAGTTTAGAAACCGCTCCTTCCATTAAAAAAGGTAATTCAGAAAACCATGCGTTGGGATTAGGAGCGATGAACTTACATGGATTTTTAGCAACAAATGCTATCTATTATGATTCAAAGGAAGCGGTTGATTTTACTAACATCTTTTTTTATACAGTAGCATACCATGCGTTTAGTGCTTCCAATAAATTAGCATTGGAACTAGGTAAATTTAAAGACTTTGAAAATACTAAATTTGCTGATGGTAGTTACTTTGATAAGTACACTAAGGTAGCTAGTGACTTTTGAACATGTAAAACAGAAAAAGTTCAAGCCCTTTTTGATAAATACCAAGTAAAAATTCCAACTCAGGAAAATTGGAAGCAATTGGTAGCAAGTATCCAAAAAGATGGACTTGCAAACTCCCATTTAATGGCTATTGCCCCAACTGGATCTATCTCATATCTCTCTTCATGTACCCCTTCACTTCAACCAGTAGTATCTCCTGTTGAAGTGAGAAAAGAAGGGAAGTTAGGACGGATTTATGTCCCTGCTTATAAGCTTGATAATGATAACTATCAGTACTTTAAAGATGGTGCTTATGAACTGGGCTTTGAACCTATTATTAACATAGTAGCAGCAGCCCAACAACATGTTGATCAAGCAATCTCTTTAACCTTGTTTATGACTGATAAAGCTACCACCAGAGATCTCAATAAAGCTTATATTTATGCTTTTAAAAAGGGTTGTAGTTCTATCTATTATGTCAGAGTAAGACAAGATGTTTTAAAAGATAGTGAAGATCACACTATTAAAATCAAGGATTGTGAGGTTTGTTCTATCTAACTATTAAAGCAGTTAGAATTTGTTAGAATTACTTGTTTTAAAACTATCTTAATCCCTAATATATAAATTAGAAAGGCAACGGTTTTGAGAAGATGCATGCTATTGTGGTTTGTGGTGCTAAGCAGTATTTAGTCCATGAAAACGAGTCTATTTTTGTTGAAAAATTAGCTGGTAAAGTTGGTCAGGAGATCCAACTTGATAAGGTATTGATGCTTGATGAAAAGATAGGCAAACCTTACCTTGAAAAAGCTAAGGTTGTTTGTGTGATTGAAAAACACGGTTTAAAATCGAAAATTAAACTAATTAAACACATCTCCCAAAAACACCACCTCAAGCGTTATGGCCACCGTCAACCCTACACCAAACTAAAAGTGGTACGCTTTATCCATGATTAAGATTAATATCTCCCAAAACTTTCTAGTTGCAAAAGGTCATGCTTTGTTTGCTGAGAAGGGTAAGGACATAGTTTGTGCTGCAATTAGTGGAATTATCTTTGGGGGGGTGGCTTGGTTTGAACCTGATAAGATTGAATTTACTGAAAATAAATTAGTACCTAGTATAGCACTGAAACTCATTGACCCAACCCCTAATGTAGCAGTTGCTTTTAGTGTTATTACAGTACAATTAAAAGCAATAGCCAATTCCTATCCTAATCACATAGTTATCAATGAAGAGAGTTATGAGTAAAAAAAGTTACTGCTACCAAATTAACTTACAGTTTTTCGCTTCCAAAAAAGGGGTTGGTTCCACTAAGAATGGACGTGATTCACATTCTAAGCGCTTGGGCGCTAAGAAGGCAGATGGTCAGATGATTAGAGTTGGTCAGATTATCTATAGACAAAGGGGAACTAAGATCTTTGCAGGACAAAATGTTGCAATGGGTAGTGATAACACCCTCTTTGCTTTGAGCGATGGCATTGTCCGCTTTCAAAAGTTTGGTAGTAAACAAAGCAAAACCCGGGTTAACATCATCAAACACCAACTAAATGCCTAAGCTACTGGGTAGCTTTATTAGCTTTAAAGCCCCCAATTACTTTGTTCAAAGTGCTCAGGATGCAATTGCTATTGATGCAACTGCTCTAATGGTATTTTTAGGTCCACCCCATTCTGCTTACCGTGTTCCTTTTAACAAGATGCAGTTTAGTTTGGGCTATGAGTTGTTAAAAACAAAGAATATTAATAGTAATGGTTTGGTTGTTCATGCTCCATATATCATTAACTGTGCATCAAAAGACCCACTAAAACAGCAGAATGCTATCAGTGTTTTAACCAATGAGATTCAGCTTTGTAACTTGGCTGGTGCTCACTATTTAGTTTTGCATCCAGGTTCTGCAGTAGCCCAAACAACCAACGAAGCATTAGATAACCTGGTTAAAGTACTCAATCAAGTTATCAATAAAACCAAAACAACAGTTATTTGCCTTGAAACAATGGCTGGTAAGGGTAATGAGATAGGCAGAGACTTAACTGAGTTGAAATATGTTATTGACAGGATCGTTGATAAAGATAGGATCGGGGTTTGTCTTGATACCTGTCACTTCCATGATAGTGGGATTGACTTTAGTGATCTAACTGGTGTTTTTAACACCATTACAACTAAACTTGGTTTTGAGTTTCTCAAAGTAATCCATTTAAATGAATCTAAAAATAATTGTGGTTCTAAAAAAGATAGGCATGCTAATATCAATGCTGGGATGATTGGTTTTGAGAACTTAATGAAGTTCATTAGTCACCCCCAAATTAAGGATTTACCTATTATCTTAGAAACCCCTTCAACTAGTTTAAACTACCCAACTATTTACCGTGAAGAGATTAGTCAAATCCGCAGCTGATTTAAAACTTACCAACCAGATGCTAACTAGTTATGCGAAGGTATTAGAACAAAACAATCTGCGCTTAACAAAACCGCGGATTGCTTTATTAAAGTGTTTAATTGAACACCAAGATTGGCATAATCTCTCCCAAATTAAAACCCACCTTGATTTAGCCAATCAACCCTCAACACTCGCTTCTCTCTACAACAACTTAAGAATCCTAGCTAAACTTAAACTGATTAACATCTTTGTAGATCCAGAGCGGTTTGAAACTTACTATTGCCTGCGCCATGCAGAGCATAACCATATCTATCTTTTTGATGAAGTTAAACAGCAGTTTTTTACCTTACCTTTAACAGATGGCCAAATTAAAACATTGCTTGAAACCCAAAACCATACCAGTAAGGTAAAGCTTAATGATTTTTATATTGTTGCTAGGGGTGAGATAAACAATGATTAACAAACCAAACCAATTTTTAAACCACCTTGATGGCTTAAAGCAACATTTTTCTGATTATGATTCACTACAAAAATCCTTTAAGAAATATCTATCAGAAAATCAAACTGAACTTAACAATTTCTTTTTTAACCAGTTTGAAAAGATCATTGTTCTGGTCAAAAAAAAGGAGTTTAAAACTGCTCAAGAGAGGTGTGAAGAGGAGTTAGCTACCCCTTATTTTTCAAAGCCATTGGTTGGTTTTTTCCAATCACTATTACAACTAATTAACCATGATCTCATCGAACAGAAAAACCAACAGTTAGCTAACATGAGTTGTGAAAAAATTGTGGAGATGGTGTTAAGTGATTACCCCAATAAACTTAACTTAATCCACTATTTGTTAGCAAAAGAAGCGAGTTTTGTAAACCCTAACCTTTTACAGCGGATGACTTTTGTGTTAACTGATCTTGAACTGTTAGAGTTAAAGCGTTTCTCTTTTTTTAAAGCCCTTAACCAGATACCTGCTTTTAAAAACCACAAAGTAACATACTTTAACAGCAAACTCAAACAGAAGTTTGTAATAACATTAGGTGAATTTGCTTTCCCCCAAACTGATAAAACCAAACAGTTTTTCCAACAACTAATTAAAAAAGTAAGTCAACTGTTTTTAAAAGAACCTGTTAGTTGTGAATTTGCTTATGAAATTATTGATGCATTACTCGTCAGTTTTTTTCCACTCCATCCTAATTTAGAAGTAAACCACTTAGCTAAAAAGATCCACCAGTATGTTAGTAAGATTGTCATTAATGAAGTTGTTGATCTGAAAGATCCAACCACTAAACTAATTGTTGATACACTTTATGAACAGTTAGATAGAGCAATTGGTGAGGAAAATTAAAATTAAGTTAGCACTAGTAGATACAAAAGATGAAGTTATACAAAGTTCTTAACAGTAAAACAACTGATAAAAGTCTTTGTTTGGAAGTTGAGATTGATCCAAATTACTGACAAGCTACCCAAAAAAAACTAGTAGGTGAAATGGCTAAATCGATAAAAATTAAGGGTTTTCGTCCCGGTAAAATCCCCCCTAATTTAGCCAGTCAGTCGATTAATAAAGCTGAATTAATGCAAAAAAGTGCCCAAAACGTCATGAACAGTATTTATGAATCAGTTCAACAAGAAGAGATCGTTGCTAGTAATGATAATGTCATTGATGATTATCCTACCATTGATTTCAAAACGATCACTGAACAAAACTGTGTACTTTTGTTTTACTTTGATCTGATCCCTAACTTTCAACTCCCTGATTACAAAAAGATAAAAGATTTAACACCACTTACCAAGTTAACTGAAGCTGAATTTAACAACGAAATTGAAAAGCTGGCAAAAACTAAAAGCACAATGGTAGATGTTAGTGATAAAAAACTAGCTAATGGTGATATTGCTATCATTGATTTCACTGGGATAGTTGATAACAAAAAACTAGCATCAGCTTCAGCACAAAACTATGAATTGACAATTGGTTCAAATAGCTTTATTAAGGGTTTTGAAACCGGGTTAATAGCAATGAAAGTTAACCAGAAAAAAACTTTAGCACTAACTTTTCCTAGTGATTATCATGTTAAGGAGTTGCAATCAAAACCAGTTACATTTGAAGTAGTTTTAAAAGCAATTAAAAAACTGGAATTCACCCCAATGGATGAAACTAATTTCAAATCCTTTCTCCCTGAACAATTCCAAAGCTTTACTTCTCTAAAGGCATTTAAGAGTTATTTTCATAAGCTAATGGAAAACAAAAAACAAGAGACAATTCTCCAGGAGAATAACCAAAAAATTCGTCAGTTCTTACTTACTAATACCAAACTTCCTTTTCTTCCAGAAGCGTTAATTAAACTAGAAGCTAACCGCTTGTTAAAGCTCCAGCAAAGCCAAGCTGAACAATATAAAATCCCCTTTGAAAAACTCTTAAGTGCTTCTAATATCACCCTAACAGAGTTACAAGATCGCAACATAAAAGAAGCTAAGGAAAATGTTACCTTTGCTTTGGTAATGAAAAAGATAGCTGACATTGAAAAGATTAAGGTTGATAATAACAAGATTAAAGCTGAAATTGAAAATGTTATTGCTGTTGAATATCCCTTTGCTAGTGATGAAATGAAAAAACAACTCTTTTTTAATATGGAACAACAAAAGGAGTTTGTGGAATCAATTATCATCAACAGATTAACAACAACTAAAATCGTTAGCTATTCAACTCATTAGCACTCAAAGCTTGTGAGTGCTAAGAAATGTGTTAAAATTTATTGAAATTCCCTAATTAACTTTTAAATATGCCCGTTACGAAGAAAAGTCAGATCTTAGTAGTTAGAGGTCAAGTCATTTTTCCTTTTGTTCCCTTTAGTTTGGATGTTGGCAGGCCCCGTTCGCGTAAGATCATCAAAGCGCTTAAAACTCTGAAAACCAAACGTTTGGTTTTAGTAACCCAAAAGTTTACTGGTGAACAAAACCCTGAGTTTAATGACATCTATCATGTCGGTACACTCTGTGAGATTGATGAGATAGTTGATGTTCCAGGGGTTGATAGTAAAACAGTAGACTACCGTATTAAAGGCAGAGGTTTACAACGGGTTTTAATTGAAAAATTCTCAGATGCAGATATTAATGAAGTTAGTTACCAATTACTTAACTCCACAGTTAAAGATGAAGCTAATGTTGACAGGTTCTTACAGCGAATCTTTCCTGAAAAAGAAGAAATTGAACAGTTAATGGAAGGAGCTGAGAAGTTTTTAGAACTTGAAAACATCAGCAAAACAGTTAATGTTCCTAAGGGTTTAAAGCAACTTGATATTATCACCTTTAAACTGGCTAATCTTGTCCCTAACACTGAAAGTATTAAACAAGCTATCTTAGAGGAAAATGAGATAGCAAACCGATTGGAAAAGATTATCCAAGCAGGGATTGAAGATTTACAGAAGATCCAAGATTATGGTAGATCTAAAAACAAGGAAACTGAGTTTGATAAACTTGACAGTAAAATTACCCGCAAAATTAACGAACAACTCTCAAGACAACAACGTGATTTCTATCTTCGTGAAAAGCTAAGAATTATCCGTGAAGAGATAGGGATTAGTTCCAAAAAAGAGGATGAAGTTGCTAGTATTAGAAAGAAACTGGATGAAAACCCTTACCCTGAAGCCATTAAAAAACGGATTTTAAGTGAACTTGAACACTATGAAAACTCTTCCTCCTCTTCCCAAGAATCAACCTTAACCAAAACTTACATTGATACGCTTTTAAACCTGCCTTGATGACAAAAGAGCAAAGATAACAGTGATGTTAAAAACTTAATTAAGACGTTAGATAAAAACCACACTGGTTTAGATAAGGTTAAAGAAAGGATTGTTGAGTATTTAGCAGTACAACTAAGAACCCAAAAAAACAAAGGTCCTATTATGTGTTTAGTAGGTCCTCCTGGGGTTGGTAAATCAAGTCTAGCTAAGTCTATTGCAGAAGCATTAGATAAGAAGTTTGTCAAGATCTCATTAGGGGGAGTACATGATGAATCGGAAATCAGAGGTCACCGTAAAACTTACTTAGGTTCTATGCCAGGAAGGATTTTGAAAGGGATGATCCGTGCTAAGGTAATTAATCCCCTCTTTTTACTTGATGAAATTGATAAGATGACCTCCTCCAACCAAGGTTATCCTTCAGGTGCTTTACTTGAAGTATTAGATCCAGAGTTAAATAATAAGTTTAGTGATAACTATGTTGAAGAAGATTATGATCTTTCTAAAGTAATGTTTATCGCTACTGCAAACTACATAGAAGATATCCCTGAAGCTTTACTTGATAGGATGGAGATAATTGAACTCACTTCCTATACAGAACAAGAGAAGATTGAGATAGCAAAAAACCACTTAATTAAGCGTTGCTTTGAGGATGCTGATCTTAACAGTGAAGAATTGAAGTTCACTGATGAAGCAATCAGCTACATCATTAAGTTTTACACAAGAGAAGCGGGGGTTAGACAATTAGAACGATTAATCCAACAAGTTGTAAGAAAGTACATAGTAGCAATGCAAAAAGATGGCATCAAACAAGAAACGATTGATGTAAACGCTGTTAAAAAATACCTTAAGAAGGAGATCTTTGATCACACTATGCGTGATGAAGTGTCTCTACCTGGAATTGTCAACGGGATGGCATACACCCCAACTGGAGGGGACTTACTTCCCATAGAAGTTACCCATGTTGCTGGTAAAGGAGAGTTGATCTTAACTGGTAATTTAAAGCAAACAATGCGAGAAAGCGCTAATGTTGCTTTAGGCTATGTAAAAGCTAATGCAGAGCGTTTTAACATTAATCCTAGTTTGTTTAAAAAGATTGATATTAACATCCATGTTCCAGGTGGGGGAATTCCTAAGGATGGACCTAGTGCTGGTGCTGCTTTGGTAACTGCAATCATCTCATCATTAACTGGTAAGAAAGTAGATCCTACAGTGGCTATGACAGGAGAGATCACTTTAAGAGGCAAAGTGTTGGTTATTGGTGGGGTGAAAGAAAAAACTATCTCAGCTTACCGCGGTGGGGTTACAACTATCTTTATGCCTGAGAAAAACGAGCGCTATTTAGATGAAGTACCCAAAGAGATAGTAGATAAACTTAACATTATCTTTGTTAAGGAATACAGTGATATCTACAACAAGCTTTTCAGTTAGTCTTTAACTAGGTTTTGGACATATTTTAAGGTAATTAAATAGGCCTGTTTTAAGTTGCTTTTAGCTAGTTTTACTAGTTGATCAATCTCAATTCCCCCACTCCACTGTTCCTGTTTACGGTTAGGTTCTAGTTTATCTGCTAAATAAACAATCATATCTAGCTTGCTAACTTGTTTTGGTGGGATGGTGTGGTTTTTGATGGCATTAATAATCATTTTATCTTTAACACCAAATCAGTTTTTTAAGATATAAGCCCCCACATAGCTATGTAATACTTTTCAACTTGGGTAGTTAGTTATCTTCAGTTCACTAGTTGCAATGTTAACTAACTGATCAACTGGTAGTTGTTTGGCCAAGTCATGATAAGCCCCAGCAACAAATGCTCTTTTAGCATCTAGTTTGTTTGCAATTGCTAGTTGTTTTGCTAGTTTACCAACCCTTAAACAGTGTTGAAACCTCTTATCATCAACCATTGCTTTTAAAGTGGGAATTAGATAGAGGTGATTGGTATTGATGTAATTTAAAACTGCCAGTGGTATCAGTTTTTTTCTAGGTTGATTTAAAAGTTTACTTGAAGCGATTTCAAGTGGGCATTTAGCCAAGTATTTAACATTAAATTGGTTTGCTATTTTTTTATTAAAAGGATAAGGTTTTCGTTCATAACAAACAAACGTGCACAAATCTTTTAACTGTTGGATGTGATCTCACTTCTCAAGTTCATTTAATTTATCACTCCCTATTAAAAAATAGATTTCACTAGTTGGATAACAACTTTTAAAGTGGTTAACAGTATTAATTGAAAAAGCGTTTTTAGTTTTAATATCAAAATTGGAAACTAAAGCATTGTTGACTGATTTAATTGCTAGCTTTAACATAGCAATCCTATCCTTATTACTAGCATGAAAATTATTTTTAAAAATACCATTGTAAGTTGGTACAAAAAAAAGCTTTTGCGCTTTTATTTTTTTAATTGCATGTTTAGCAATGTAGAGATGGGCATTGTGAATAGGGTCAAAAGAACCACCAAAGATAATAATTTTTTGCTTCAAAAAAGATCTTAAAACATTTAATTCTAACTAAAATAATTGTGACACAAGAGCAATGATCCAAAAGGAGATGGAGATCTATAACCTCTTTACTTTTCAAATTGATCTTGATAAAAAACTGTTGTTTGAAAAATCAAATGATCAGAAGAATTATTCCAAGATTAGAACCCATTATTTCAAGCATAAATTCAAAAATAAAAGTGCTGTTTTTCTCAATAAAAACCTGATTAAAAATAGCTTAAATAAGGTTCTTTTAAACTTTTCTGATTTTGTTAGTGGTGCTGGAATTGATACAGTTTTTAACCAAATCATTGATGAAGATCCTGAGGTTTTAAACTATTTAAAGCAAGTTAAAAAAGATCTTTCCAAAGAAAACAACGCTACATCCCAACTAACCTTTAATGTAACTATTAACCCTAAAAACACCCTCGCCAATTTTTTTGAAGGATTTAATATTTATCTCCATTTTAATGAAGAAAACAATACAGTGATAGGTAGTTTCTCACTACAATGACACATCAAAAAAACTGATCTGTTTTCTGAAACAAAAAACATTGCCATTAACAATTTAATTCACACTTTTTGTAAGAACAACATGCATGAAATTAGTTTTATGCAGATCATTAATTGTTTTTCCAAAACAAAAATTAATAAACATGGTGAAATTGTTTTAAAAAGTTGTGCTTTTAAACAAAAATGACAAAATGTTGTTGCTGAAAAATACCCATTCTCAACTGCATCTAAAGATTTGGAGAAAATTAATGATTTTTTTGATGCTCTTTTTGTAATGCTATTATTGGTATGTCACCTCAATAAAAACCTGTTATGATTATGTGAAAAAACTGATTTTTTTGAATGAAAACCAAGTCAGAAAACTGCACTTTTTAAAGCTAATGATAGTGGAGCATACTTAGCTAGAATGCTGCTCTTTTTAAACGATTGGTACAACGAAAATCAAGCAATAACAACTGCTGACATTGAAAATGTTAATGAGGTTGAAGACATAGGAAAATTAGTTGAAAAATACAGCACTAATCAACCCCAAAAGTTATCGTTAAATTCAACAGTTTATGTATTACAAACAAAACAAAAACAGTTTTTTTTAAAAAATGATTTCTTTTTTAATAATAACGAAGCAAAGCTGTTTTTTCTAATAACTATGAAGCCCAATGTTTTTGGTCTTGATGATACAGCAATAGCAAATAACTTAAACCTTAAAAAAATTAGTGATTTTTTTAAAGAGATTGATTTTAATGATGAGGATATTCTAAATGACTTTAAACAAGAACAGGAAAAGTTATTGGTTAGAAGAACATTTAACCAACTGTTATTTATGAACAAAAATACTGAAATTCTCAGTGTTGTCAATGACAAGCAAAAGTCAGTAATCCATAACATTGTTTGAACCATTACTTATAGTAAAGCGATCATGTTAAAAGCCTTTGATTATTCCAAAGCCTTTGAAAAAAACCGAACGAGTGATCCTTCCTTATTGCGATCTAATTTAACTGTTATCAACCGCTTAAGATACCTCAGTGAATACTTTCAAAATGCCTCACTTAAGTATGATCTACTCTACACTAAGGCCAAACAATATATGCAAATTGATAAATTTATCAACGACATGATTCGCAAGGTAAACCATGAGGATGAAATCTTTGGCAAATTTAAAGAAAGAATTTATTTAAGTTTGGGAATTATTAGTGCGGTAGTGTTTGGCATAGTTGAATTTTTTAACTGTGTATGGACTATCTTAACTGTTAGTCAAGAAGTGGTTGATAAAAGTGTTTTGGATCCAAGGAATATTATCTTTATTAGTATAGGTACTATTTTGGTTTTATTTCTTTTGGTTACTATCTTGGTTTTTATGACAAGAAGACTTTATCTGTTTGAAATTAATAAAAAGCATAAAAATTAGGAATCATGAAATTCAATAAGTTAAACCTAAGTCATTGTATCTCTTTTTACATCTCAGAAGTTTCAGAAGTTTTCTTTGAAAGTATCAACCAACACCCTAGTCGTGATTTTGTTAATAACATTCTTCAAAAGATTAAAACTACTTTAAGTGAAGAAGAGCTTGAAAAGTTAAACAGTATTGAAGAAGTTACTAAGGATGAGAAGATTGTGATCATGCTCAACCATGTTTTGAAAAAGATTGTTTCTAAAACTGGTAGTTCTAAGTGTGACTTATTTAATGTAATCAAACAAGATAGGTTTAATTCTCCAGTGTATATCCAAAGTATTAATGCTTTTGAAAACAACCTTATTAACAATGAATTTGCTGAAAGAAGGTATGACTATTTGATTGAAGTTAATAAAAATTCATACCTTAAAAAATTTGTCAATTCGATCAGAATTTCCTTCTTCTTAGATTTAAGAGCACAAATTTTGTCAGGTTCATTTACCCTTAATTTAGTTAATAAGTCAATTGAAAAACAAAAAAGACAGAAATTTTTAAAGATATTTTTGTTAATGCCTTAGTTAAGCATTTTATCTGTAATCAACTTTATCCTATCTCTTTAAATTCCTTTATTTTTGACAGTGAAAATCCTAGCAATAAACTCGCTCTAAAAGAACGGATTAAGCTTTTAAAAACAAACTGAAATTCCCTCTTTTTTGATAAGTTTTACAACTGTTTAAACAATAAAAATAAGCAGCAACTTCAAGAAACTAGTGATGAGATGTTTTATGCAGTGATTAACACTTATTTAATCATGTTAATCTCTGTTGAAGAGTTAAGGGTTTACTTTACAAGTAAAGAACCAGCACTGATTTTAAAGGTATTAGATAAAAAAAACACACTAAGAGAAGATCCTGATCAAAACCCTGAAACTGATTTGTATGAACTAATACAGTTTATAGAACAAAACTACTTAAAAAAAGATAAAAAAACCAGCTGAAATAAAAAAAAGGTACAGGATCTTGAACAGTTACTTGAAGAGATTAACAAGATTAATTTAGAAACAAAAAATGAATCTTTAGCTTACCCTGATGAGATAACAGAATTGGAAATTGATAATGATAATTTTGTCTCTACAAAACAAGTATTTAGAAACCAATTAGAATTACAACTTTTGCATGGGATTGTAATTAATCCTGAGAAGTATGGAATTGGCATGTGAAGTAGTTATTTTGCTGATTGAAGTGAGTACAAAAATTTAATAGAACAGATGCTCAATCCCAAAAGTGGTAATGATTTTTATCAGTTTGAAAAGGACATAGATGAAAGCATATGTCAAATTAACAAAAAATATCTAACTTTTATTAGCAGTGATAGTAATACCTTTTTAATAGTTAAAAATGATGATGTAAAAGTTATTTCTAACTATGTATGAGCACAACTTTTCTTTGAAACAAGAAGGTGAATTATCAATGACATTGAATTTGACTTGTATGAAAAAGGATTTGATAAAAGCCACTTTTCACGTAACATTGCTTTACTTGAAAGTCTCAGTTTTAGTTGATTAGATCCTTTTTATGGTTTGACATCAATTAAAGAGATCATGCAGAAAATAGATAGTAAAAGTAATCTTAAAACCTCGATTGAAGAGATGGTAAATAGGTTTAAACATGAGCAGCGAATTAATAAAAAGGACAATGAAAGGGTGTTGATGATTTTTGCTTATATTGCTGCTTTTGTAGTAGGATTTATCAATTTTTTCTCAATGGTCTTTACTATTCTCACTGTAAGTGATCTAAATGCTGGGCTTACTGTACCTAACATCATTGTAATCAGTATCGCTAGTGTTTTAGCTTTTATTTTGATTGTGATTGCTGTTTTATTTCGTTTTAAATGAAAACACATTAAGCACTAATAATGCAAATTAAAGTTATTAATGAAACAAATAAAACTGTTCAAATTTTTCAATGTGCTAAAGTAAAACATAGGGCATTAGCTTGATTGTGCGATGTATTTTTATTAGCTATTGTACTTGTGGTTATCTTTTTAATTACTCAAGCCTTTAGTGATAACCGTTTTCTTTTATTTTTAGTTTTAAGTTGTTCACAAACAATCCTGTGAACGGTTTATTTCATTTTTTTACCATTTTTTTGGGATGGTAAAACTTTGTTTCGCAACCTTTTGAAGATTAAGCTATTTGCTTTTGACAAAAGGTTTTTGAGAATAATGATCCATGAGTTGTTTTTATGGATTTTGCTTTCAGTTTTGTTTTTAGTGATTGCTAGTTATTTTTTTATTAACCAAAATAGCAGCGAAGCGCTTAATTTTTTCACTAATTTAGATAAGCCTAATGCAATAGCGATAACAATTAGAACCATAACAATTTTGATTAGTTTTTTACAACTGATTTTTATTGGTTATTTTTGTTTTAGTTCTGAAAAACAAGCTTTACAAGAGATACTGTCAAACACCTTTATGGTTCAAGAAAAACACACTTTAAAATCAAAACCAACTAGCTTAAAAACCAACAATCAACCTGATCCAGCTAATTTACCAGGCGTAATAGCTCTTGATGAAGTGGAAAAACTCATTAATTAATAACAATGAATGAACAACAAAAACAAGCAATTAGTTGTGGAAAAGGGGTTAATGTTGTTTATTCTGGAGCAGGTACTGGTAAAACAACAATTATTACTAATCGCTTTGCATACTTGGTTAATAAAGAAAAAGTTGATCCTAGCAGAATTTTAGCAATCACCTTTACTAAGAAAGCTGCTAAGGAGATGCAGTTTAGAATCTTGAAACTAATAGATAGTTCTTTAGCTGAGAAAACAAATATCTATACATTTCACAGCTTTTGCAATAAGTTTTTAATTCAAACATTAAAAAAGCGCTTTATCATCGATGATGATATTAGCTATTTCCTAAAGGAATTTTTAGCTGATTCAAAACTCGATATCAACCTAGCGAAACAAATTATTGATAACTTTAAAAATACTTTTGCTGATTTTGAAATAAATAAGTTGGATCAAGATGAAAGGTTAATTAGTTTATGTGAGCATTCACTTCTAAATAAAGATGAAGAATATTCCACTTTAAAAACCCAACTGATTAATGCATTCATTAGCTATGAAAAGAATAAGATATTAAACAATAAACTTGATTTTCATGATCTTTTAATTAAAACTTGTAATTTATTGAGTAATGATAATGATTTACTTAATCAGTGGAGTGAACAGTTTCAGCATATTTTAGTTGATGAATTTCAAGATACCAACCAAATCCAATATGAACTGATCAAGATGTTAGTAACTAAAAATAAAAACTTGTTTTTGGTAGGTGATAATAACCAGATGATTTACCGCTGAAGAGGGGCGGTAAACGGGATCATAACTGCTTTAAAGCATGACTTTAATGTTCCGAAAAGCAATGAATTCTTTATTAATCAAAATTACCGTTGCGATCAGAATATTTTAGCAGTTGCTAACCAAATTCTTTTAAAAATTATGGCCTATGAAAAACAAGTTAAAACTGAAAAAAATCTCTTGTTTTCAACTTTAAATTCTGATAAAAAACCTGTTTATTTTCAAGCTGAATCAGTTGAAAATCAAGCCAATTGGATCTTCAATAAAATCAAAGCACTAAACCAAACAGAAAAGATTAATTTTAAGGATATGGCCATCTTGTTTAGAAAGAACAGAGATATTACTACTATGGTTGAATTGATTGAAGCGGATGGAACAATTCCCTTACCCAAACAAAAGAGTTATTTTAACCAACTAGTAAAACTCCAGCGGGTTTTAATTGCGATTTCAACCAGAACAAATCTTGATATTAAAAGAGCTTTGCAAGCCCTAAAAATTTGATCAAATGATTTAAAGGAATTGTGAAAACAGAGTGATAAAACAAACCTATTTGATTTTCTTAAATGATCAGAATTAAATCAAAAAAACCATAGTTCAAAACTTAAAGCTACTGGTTATTTTAATCTGCTGATTAAGTTAGCAGAGGATCAGCAAATTAACCTTTTGTTTACTGAACTGTTTAAAAAACTCAAAGTGGATCAAACTATTGAAAATCTGCTTTGAAAAAAACTAACTGAATTTCAAAAAGATAAAACTGAATTTAGCTTATCAGAGTTTATTACTAGCTTAGCATTGGAATTTGACTCAATTATTGAAAACAGCAGTGATACAATCAATTTGCTAACCGTTCATGCAGCAAAAGGACTTGAGTTTGAAGCTGTATTTATTTATGGCATGAATCAAGGGGATTTTCCCTTATTTTTAAGTCAAAATCAAAATGACGAACAACATTTAATTGATGAATTAAAACTGTTTTATGTTGCTATCACAAGAGCAAAACGTTTTTTGTTTATCACTGCGGTTTTACAAATAAATAACAATTCTATAAAACCATCTAGTTTTTTAAATTACATCAATAAAAGTGAGTATTTAGACATTGCTACTATTAACTATGTATTAGAGCAGGATGATGATTTTTTTGATTCAACTAAAAAAACAGACTATACAAAGAAACTAAGAAAAGAAAGTTTAGACATTATAGTGGGTGATTTAGTTACTAGTAGATACTTTGGAAAAGGAGTTGTAGTTGAAGTGAGAGACAAAGAGGTTTTAGTAGCTTTTAAAGACACACGCTATGGGATGAAATGGATCTTAAAAAACCATAAATCACTAACAAAAGCTTTATATTAACAATGGTTGATAAAAACAGTTTAAGAAAATTAATGCTTCTAAAAAGAGCAGAACTAAATGATCTTGAAAAATCGCATTTAGATCAAAAGATTAACCAAAAATTAATGGCTTTTTTAATAACAAGACCAACAATTAAAAATTTAGCACTTTACATTCCCATTAAAAACGAAGTGGCTTTTTTAGATAACTTTCTAGATTTTCTTAAGTTAAATAAAATTACAAGCTGTTTTCCTAGTATTGTTGATCAATTTAACATGAAGTTTATTGATCAAAATAATAATGAAATTAACCCTAATGATATTGATTGTTTTTTTATCCCTTTATTAGCTTTTAATAAGGCAAACCACAGGATTGGTTTTGGTAAGGGTTATTATGACCGTTATTTATCATTAACTAGCAAAAAACAACTAAAAATAGGGATAGCATATGACTTTCAATATGCAGAATTCACTAATGATCCTTGGGATTATCAATTAGATTTAATTATTTGCAATGGATAACATAAAGGTTCTTTTTTTAGGTGATGTTTATGGCAAAGCTGGTAGAAAGATTATTAGTGATCATCTTCCCATAATTAAAAAAAAGTATCAGTTAAATCTAATTATTGCAAATGCTGAAAACACTACTAATGGTAAGGGTTTAAGTTGAAACCACTACCAAATACTAAAACAAGCAGGAATTGATTACATCACTATGGGTAACCATACCTGGTTTCAAAAGCAAGATTTAGAACTTGTTTTAAACCAAGTTGATGTTATTCGCCCACTTAACTTAATGCAAGATTTTAACTATTTTCAGCTTGGCAAAGGGAGTTATTTATTTAGCTTAAATGGTTTGAAAATAAGGATTACTAACTTGTTAGGAACAAGTATTAACTTACCATTTGCAATAACAAACCCATTTGTGGAATTAAAAAAGTTAGTTTTAACTAAAGATTGTGATCTTCATATCGTTGATTTTCATGCTGAAACAACTAGCGAAAAAAATGCTTTTTGCATGGTTTTTGATGGTTATGTTACTGCTATCTTAGGAACCCACACCCATGTTCCTAGTAATGATTTAAGAATCACTCCTAAAGGAAGTGTTTACATTACTGATGTAGGGATGTGTGGTCCTGGATTTGGTAGTGTTATTGGTGCTAATCCCAAGCAATCAATTAAGTTATTTTGTACAGGTGAAAGACAGTTTTTTGAGGTTTCTAATTGTGGTGCACAACTCAATGGCGTCTTCTTTGAAGTTTGTTCAAAAACCAATCAAGTTGTGAAAATAGAACAGATTAGAATAGTTTTAGATGATGAAAAGTATTTAGCTAATGACTACTTTAATTTAGTTGAGTAATCTTGCTTTCCTTACCATGTAAAATTCTGATTATGTTTGCACGGTGTAATCAGAAAACAACCACAGTTAAAGGTCAATATCACAAGCAAAAAAATAAAATGATATACCATTCATTTTGATAAGTAATCGACTTTAGAGGATCACTGTTAAAGAAGTATAAATAATCAAGTCAGGGTATTAAGATGATTACAGCTAACACAAAGAATGTAATAAGACTTGCTAAAGAAACATACTTAGTTATTAAAGTAATCATTATCCAAATTAAAAGACAGATTAAAAATCATCATAAAGATATTGCAAGTAAAGATCCACCAGTAGTAGCAATTGCCTTACCACCCTTAAACTTAAAATACAGCGGAAAGATATGACCTATTGTAGCTGCAAAACAACTTAAATAACTTAAAAAATAGGTGCTTTGATACACTTTTTCTGTTAAATAACCTTGTAAACCAAAACGGAATAAAATTCAGGTTAATAAAAAAGCAAAAAAACCTTTAAATGCATCAAAAATAGCCACCAAAAAACCAATTTTTAAGCCAAAAACACGCATTGAGTTAGTAGCTCCTGGGTTTTTTGAACCAAATTCCCTGACGTTTTTCTTGAGTATTTTGCTGAAAATATCAGCAAAAATAATTGAACCTAACAGATAACCAGAAGCTAGGCTAAAAATGACCAAAATGGCAATTGCACTAGCTTGATTCATAGCTTGTAATTATCTTATTTAATGTTTCAATTATCTTAAGATCCATCAAGCTAGGTTTTTGTTTGGGTTGATAAACTCTCAATAGGCATTGGTAGTGTTTCATTAAACAGTGTTTATCTTTAAAGTTAATTAGCTTTGGTCCTAAAATAACCAATTCCTTTGTTAATTTAAACGGTTTTTTAGTTTTTCAATAACCAAAATAGGATATATAAACTCTCGATCCTGAACTAGTGTTTCATTAACAATATCCCAACTGTTTAAACTTAAAAAACTACGTAATTCAATTAAATTAGATTGGGGTTGGATTACAAAACGATTAATAAAGTTTTCTTTTTGGCTAATTATGTTAATGATTTTCAATCCACCAAGTCCAGCAATTACCCCTATTTTGGGATTAATATTTAGTTCTGGAAGGCTATTAAATCCATCACTAACAAAGAAGTGGATATTATTGTTATTTTTAAATTTTTGGTAATTACTTAATAAAGCATTTTTACTAATGTCACTGTTAACAATGGTTAGGTTTTGATTAGTTTTTATTAGATAGGATGTTAGATAGGAATGATCACAACCGATGTCATACACTAGCTTAGGATTAAATGATTGAACTAAATTAGCAATTGTGCTAATCCTTTTTTTCATTAATTCTTAGAAATGACATACTAATAGGATTGTTTCTAACTGCATGTCTCAATTTTCTAATTGCTTTGTTTTCAATTTGTCTGATCTTCTCTCTAGGGATCAAAATCTTTTGACCTACTTCATCAAGTGTTTTAGGTTCATTGTAAGGGGGCATGCCAATCCGCATTCTAACAATTAACTCTTCTTGTTCAGAAAGATTATTGTTCAACAATTCATCAATTTTTTCTGAATTTGAACGGCTTTCGGTAAACTCGTCAGGAGTTTGAGCGTCTGTGTCTTTAACAAAATCACCAAACTGGGACTCTTCATCATGTCCAACTGTTTTATCAAGCGAAACTGGATCTAAACTTAACCGTTTAATTTCAGCAATCTTTTTAACATTAAATCCTTCAGCTTGTCCTCCCATCTTTTCAGCTAACTCCTCATCAGTAGGTTCTCGCCCTAACTCTTGATACAAAGCCCGTTCTGCTTTAGCTAAGCGGTTAATGGTTTCTACCATATGAACAGGGATCCTTACTGTTCTTGCTTGATCAGCTATTGCTCTTGTAATTGCTTGTTTAATCCATCAAGTAGCATAAGTTGAAAACTTATTCCCTAAAGATCAGTTAAATTTGGAAATAGCTTTTAAAAGCCCCAAATTACCCTCTTGAATTAAATCATTAAAATCCAACCCTCTTTCTAGGTGTTTTTTGGCAATAGAAACAACTAGTCTTAAGTTTGAAGTAACCAACTGATTAATTGCATACTTACGTGACTCTTCATCAGTACTATTTAAAACCTTGGCAATCCGCTGTTCAGATTCAAAATCTAACATTTTAGAAAAGTCAAGTGATCCTAAAAAGAACCTAACATTATCATCAACCTTATCACGGTTAGAGATGTTCTTACTTGTTATCTCTTCAATATCTTCATCAATAATTGAAAGGTCTTGGTTAGCACGAAATTCATGGATGTGCTCTTCAACATCGTGTTGGAGTTGAATCCCCTTATCACGTAGTTCATCCAAAACATAGATAATTTCATCTTCAGGTAGTTCAAACTTAGCGAGAACACTAATAATTTCCTTATTTGAAAGCGTGATGTTTTTTTGTTTCTTTTTGCTTTTCTTTTCTCAAGCTTCTGTTAGAAGGTTGATGACTGTGTTGGTGTCATCATTTTCAATGTGGCTTTTTAACGATCCTTCTAAGATATCAAATAACTTCAGATTATTTTTTTCTTTTAAAGGAGCGTGTTTAGGTTTTCTACCCCTTCTTTTTTTAGGAACATCACTCTCCTTATTTTCATGAAGGGTTTTGAGAAAAGCGATGTTTTCATGACGGTGTTTGCGTTGCACTTTATTGTCAACATAAATCATCTTTTTGTAAGGACGATTCTTTTCAAGAATACGCTGTTTTTTAAGTTCAGCAATTAATTCTTCAGATAGTTCTGGTTTGGTTGAATTGGGTTTTTCGCCTAGCGTTTTTTTGTCAGTGGACATTTAGCTCAATGCGACTAGCATTATAATTTTAGCTTATATAACTTTTATAGCGTTAACTTTAAATTTTTTAAGCGCTGCTTTTGTTGTTTTAATAACGTTTGAAAGGTCTTTAGATAGTTTTGAAAATCATTAGCTTGATAACAAGTTAATTCATCTAAAAAAATATCTTTAATTTCCAATAACCTTGCTTTATTGTTTAAAAGGTAATTTTGATCTAAAACACTTTCAAAACCAACTCAGTTAGCAGTTTGATTTTCACTCCAATAGATCCTTGCTTTTTCAATAAAAAGTTCAACATCAAACAAATTAAAGTTACATTCCTTAAAAACTTCATCTAGATGGGATTGTTTTAATTCAACCAAAAAGCGTTTATCAATTAAAGCAAACGCAAAGATTTCAGGTTTTCACAACTCATTAACTGCTTCTTCAAAAGCTGTTTGCACTAGTGCTGCAGTGGTTTTTTGCAATTCTTGTGGTGGTTGAGCATGGCCTGAAGTATTTATGTAAAAACGGTTGTTATCAAACTTAGATACAAGTTTTTCTTTGTGTTTTAAAACTGTTTCATACAGAGTTTTTTCATCACTATATTCAAGTAGTTTAACCAAATTTTTAATGAGAAAAATTAAAAAACTATGGTCGTTTAAAATTGTTTGGTTTTTTGTTAAAAAAGCAATGATTTTATTAGTAATAACCCTTTGATCAAGTTGTTGCTTTTTAAAAAAACTAACAAGATATTCAATTAGATTTTGTCTTTTGTTTGCTTGTAATATAACTTGCTCACTACCCTTGTTTAAATACAGTTCATCCCAATCTTTATAGTTGTGTTCTCACTGAACAATTTCCACAATAAAATTGTTGTTATTAAGTTTTTCAATTAAGCTAAACACAGCATTTTGGCCACTAGCATCATTATCAAGTGCTAAAACTAAGGTTTGTAACTCCTTAAAGTGAGCTTTAATGGCTTTAATTTGCACATCATTTAATGCTAATCCCATTAATGCAACAGCTTCAAACTTGGAGTTTGTTAGTGTAAAAACATCAAAATAACCTTCCACAATAAAGAGTTGATTGAGGTTTTTATTTAACCTGTGAAAGTTAAATAACAGCTCCCCTTTTTTAAAAAATTCGTGATCAGCACTATTTTTATATTTCAGTTTGTTGATGTTATCAACACTTCTTGCTGAAAAACCAACCGGGTTACCATTAAAGTCATGGATAGGGATCATAATCTGGTTTTGAAAGGTAGCTTTTTTGGTATTAAAGTCAAAAAAGCCAAGACCTTGCTGGTTAGTTTTTGAAAAAAGATACAATTCACTCGGTTTTATCTTTGGATTAATGAAGGGGTATCTTTCCATACTTTCACATAGATACTTATCTTCATTGTGAAAAGCAAGTCCTAGCTGAAACTGTTCAATTAATGTTTTATTAAGCTTTCTTTTTTCAACTAAATAATTCATCCCATTTGGGTTTGTTTCTCTTTTTAATCTGGTTTGATAATAAGTAATTAAAGCATTGTTTATCTCCCAATATCGTTTTTGTTTTGGATCAACTTTTGTTAGTAAATTACTGTTCCAATTTTCTAACTTAATTCCACAAATTTCAATTGCTTTTTTAAGTGCAGTTTTTCAATCTAACTGGTCATGCTTTTGGATAAACGCTATTCCGTTGCCAGCTGCATTACAAGCCCAACACTTAAAGATGTTTTTAGAACTGGAAATGGACATAGAAGGATTTTTATCATCATGAAAAGGACATAAAGCAAGTAGACTATTACCCTTAGTTTGGATTTTAACCCCGTAGTGTTGAATAATTTCAGTAATTTTAATCTGCTTTAAAAGTTCATCTAAACTGTTGCTTTTATTGACCATTTTTCATGAATCATTGTGGTAATTCTTTGATAGCAATTCGCTGTTGAACTAAAGTATCTCTATCTCTGATGGTAACTGCATTATCTTCTAAACTTTCAAAGTCAAAAGTGACTGCATACTTTATTCCAATTGCATCTGCTTTTCGATACCTTTTACCAATACTACCAGTTGTCTCAAAGCACACTCTTCACCTTGTTTTTGCTAGCGTTTCAAATACAGAAAATGCTTCTTTTTTAAGTTTATTTACAAGTGGTAAAACAATAATTTGTTCAGGACATAAGTCAAATGGTAAACTCAATACTTCCCGTTCTTCCTCATTAATAATTTCTCTCCTATAACTACTGACAATTAGTGCATAAAATAACCGTTCAATGCCAACCGCAGGTTCGATTATGAAAGGTAAGAAATGTTGGTTTAATTCGCTATCAAAAAAACTCATGCTCTTTTTTGAAAACTCTTGGTGTTGTTTTAGATCAAAATCACCCCTGTTAGCCAAGCCTCATAACTCCTTTAATCCGTGGGGAAAATTAAACAAAAAGTCAGTAGTTTTTTTGGCATAATGAGCCAATTCTGATTGATCATATTCATGTTTTTTTAACAATTCTGGGTTAATTTTTAACACTGTTTGTAGAAACTGTTCTACCATTATTAATTGTTTTTCAAACAGCGAATTTGCATCATCAGGTTTACAAAACCACTCGATTTCAAACTGTTCAAACTCTCTAGTTCTAAACAAGAAGTTACCTGGGGTGATTTCATTACGAAAGCTTTTACCAAACTGGGCAATAGCAAAAGGTAAATTACGCTTCTTAGCTTGCAAGATATTTTTAAAGTTAATAAAGCTACCTTGAGCAGTCTCAGGACGGAGAAAAACAAGGCGTTTATCGTTGTTTACAACCCCGATTTCAGTTTGAAAAAGTAAGTTAAAATCCCTCACTTCTGATCAATTTTGGTTATGACAATTAGGACAGTTAACTTTAAAACTATTCCAATCCTTTTTAGCTTTTATTTCAGCATTAATTTGATCAACACGAAAGCGGTATTTACAACTCTTACAATCAACTAAAGTATCAACAAAGTTAGCTAAATGTCCTGATGCTTTTCAAACCAATTCGCTGAGAATAATAGGGGTTTCAACTAAAAGAACATCAGCTTTATTTTTAATAAAAAAGTTATATAAAGCTTGTTTGATTTGTTGTTTTAAAACTGCACCTAATGGACCAAAATCCCAACTATTGTTTAAACCGTTGTAAATTTCACTACTCTGAAATACAAAACCATGTTGTTTGAGAAACTGAACATAAACTTCTTGGTTGTAAACTTTAGCCATTGTTAATTAATCTTAAAAATTAGTTTTTTCAAGCAAACAAGATAATCCCTAAAGCAACACTTACATTTAAAGAATTGATCTTATTGTTCATAGGAATTTTGATTCTACAATCTGCATTCTTTGTTATTAGTTGATTCACACCCCTATCCTCATTGCCAACAATTAAGATTTTTTTAGCAAAATCAACTTTGCGATAATCAATTGGTTTTCAAATAGGATCCAGAGTTGAAACAACAGTTCAAAATCCTATTTCTTTTAGCTTAGTGATCGTATAACTTAAGTTAGCTACTTGCACTAAGTTTTGATAAAAAACACTACCCATACTAGTTTTCATGACTGTATTGTTGATAGGGACTTGGTTATTCTTTTTAAAGATAATTCCATCAACCTCACTAGCCAAACAAGTTCTTAGTATAGCACCAAAATTATAAGGGTCTTGGATCTCATCTAACATCACAAGTGTACTGCACTTCTTATTTTCAACTACTTTAACCAGTTGATCAAGTGGAATTAAGAGTTGGTTAGTATCTAAAACTGCTACCAATTCCTGGTGGTTAATATCTCTATACTGATTGTTAAATCAGTTAGTTGAATGGATTTGAAAATTAATTTTTTTAGCTTCAATTAAAGGTATTAGTTTTTTGTGACGAATGGAAATGTTTACCAATTTAATGTGAACTTGGTTATTAATCGCTTCCTCAAAGGCTTTAACACCAAATAAGCAACTTTGTTGGCGTGGTTTTTTCATTATTACAGCAACATTTTTTTAATTAGTTTTTTTCTAATTTGATCAGCTTTATCCATTGCTTTTTGTTTGTGTAATATTGATCACTCTTTAATTAAACGCACATTAAGTTTAGTATGGATAGATTTAAAGCTAATTCCTAATAAATCAATTGATCATTCCAAGAAACTAAGTTGTTCTCTCAGCACACTAAAGTCCTTAGTTTTAATACTTGTATTAATTTTTTTTATTAGTTTTCAGATAGCAGCAACTGCATTGGCAAAGTTGAGATTATCAAGTAAAGCTGAAAAAACTGGATCATATTGCTTGGGACTAATCAACTCAGATTGTTCTGAATAAACTAATCAGGTTCTAGCAACATTAAGTGTTTTTGCAATCCTTTGAATATCATTATTAGCTTTTTCAATCAATGATTGGTTTAGATCAATAGGATGCAAATAGTGTTTTTGGTAAAAGATCCAACGCAAAACACGAAAATCATGAAAGTTAAGAAAATCAACTGCTAACAAGAAGTTCTGCAATGACTTTGACATCTTTTGGTTTTCAATCATCAAATGACCAATATGCATCCAATGTTTGGTAATGGGCTGGTTATATAAAGCCATGTGTAAGGCATTTTCATTTTCATGGTGGGGGAACTTTAAATCAACTCCTCCTCCGTGGATCGTGAGTTCATTTTTGAAACAATAATCAATTAAGAAGGCACATTCAACATGTCAACCTGGTCTGCCAAGTCCCCACTTACTATTTCATTTAATCCCTGCAGTAGTAATCTTTCAAAGTACAAAATCCAATTTGTTCTTTTTATCAGTATCAGTTTCTTCTAAATGCACCATTCGGTTAGCTAGATAACCATATTGCTTTAGTGAATTAACTGCAAAATAAACGTTGTTTTGAGTTGAAACATAAGCATGGTTTTGATTTACTAATTGATCAATATAGTCAGGGATTTTATCGATTTTTTCAGTGATTTGAATATGTTTAATAGGCAGAATATTTAGTTTTTTTAAAAGTGATTTGTAAGCAGTGATTTGTTGTTTTGTAACAACTGATTCGCTTACTTCTTGTTGTTGAGCAATCTTGATGATCTTATCGTCAATATCAGTGATGTTTTGAACAAAATTAACGGTATATTTAGCCTTTTTTAAAACTCTATTTAAAACATCAAAAACAATTAATGGTCTGGTGTTGCCTAAGTGCAAATCGTTATAAACTGTGGGTCCACAGAGATAAATGTTAATGGTTTTTTGAACTAGTGTTGTTGGTTTTTGACTAACACTGTCAACAATCATAAACCTATGTTCAAATTGCTTCATTAATGATAGGAATGTTCAGTTCTTTGGCGTGATTGATTTTTCTTAAAGTTGGTTTGTTTCCTGCAATAACAAAGTCAACTGTTGGTTTGACTTCACTTGCAAACTGGCAATCAAACTTAGCTGATAATAAATCCTTAATTTGGTCACGACTAATGTTAAAGCTACCGGTAATAAGAAAGCGTTTTTGAAAATAAATACTGTTAGTTTCAAAGTTAATTTTCAGTGGTAATTGATCTGTTTTTACTTGTCTTAATTCAAGTTGTTCAATTAACTGCAAATGGTTAGGGTCATGAAACCAGTTATACAATGATTCAGCTACTGTTATTCCTACATCATTTAAACTAATTAAGTTCTCTAAGCTAGCATGCTGTAATGCTTTGATATTTTTAAAATGATTAGCTAAATTCTTAGCTAATACACTACCAACATGCTTAATACCAAGTCCTGTTAGTAATCTAGCCATTCCTTTTTGTTTTGAATTTTCAATGTTATCAACTAACTTGTTGAAAAGTTTATCACCAATCTTCAGATCTAATTTTAAAACTTGGTTTTTCTTGTCTTTTAAATCATAAAGATCAACTATAGATCTAACCAAATTGTGTTCATAAAGTTTGGTAATAGTATTAATATTCAACCCGTTAATGTCCATAGCAGTTTTAGAAACAAAATAGTTAATTAACTGGATGTTTCGCTCCTTACAAGTTTCATTGGTACAATACTGATCAACTTCATCAACTATTTTGACTAGTTTTGAATTACATGAAGGACAATATTTTTGCTCTTGAATTATGATAGTGTCATTTTTTCTTTTTTCAAGGTTTACCTTTAGTACTTTAGGGATAATTTCCCCAGCTTTATAGATAACAACAGTGTCATTGATCCTAATGTCTTTAGTTTTAATGTAATCAAAGTTATGTAAAGTAGCAGCTGTTACTTTTGTTCCATCTAAATTAACACTTTCTAATTTAGCAGTATAGTTCACTCTACCAGTTCTACCAATCGTTATAAGAACTGCTGTTAATTTAGTTTGAACAAATTTAGGACTAAATTTAAATGCTATTGCCCAACGTGGTGATTTACTTGTAGCACCTAATTGTTGATAAAAAAGCAAGCTGTTTAGTTTAATAACTAAACCATCAAGATTAAAAGTTAACTGTTCTCGTTTTTTGTCAAACGCTTCCAAGTAATTAATTAATTGAAATTTATTTTGAAAAACCCTGATGGTATCACTAACTGGGAATTTTCACTGCTTAAGCTGTTCTAAAACCATAGTTTGAGTAGTGATTGACTCTTCCAAACCATTAGGGATGTAATAAAATAATGCCCTTAATTTGCGCTGTGCAGTGATTTCACTGTTTAAATTACGTATTGTACCTGCTGCTAGATTCCTTGCATTAGCAAATGGTTTTTCAAGTTGATTGTTAATTGCTAAAAAAGTTTTTTTATCAACAAAAATCTCACCCCTAATCTCAATTGTTTTTGTGAAAGGGATTGTTAAAGGGATAGATTTAATGGTTTTAACATTATTTAAAACATCTTCCCCAACACTTCCATCTCCTCTGGTTAAAGCATGAACTAAAACACCATTTTTATAAGTTAGAGAGATACTAACTCCATCAATTTTAGGTTCAACTACAAATTCATTTTTTGAGTTTGTTTGAAAGTTAATATTATCAATAAAATTAGCAATTTCTTTAGTTGAAAAAGCATTTTCCAAAGAGAGCATTGGACTGTTATGGTTTAACTTTTTAAAACCCTTCACAGCTTCTCCTCCCACCCTTTGGGTAGGGGAATCAATTTGGATTAAATCAGGATGATCTTTTTCTAATTGTTGGAGTGACTTATACAACATATCATACTCAAAATCATCAATTAAAGGTTCGCTTAAAACATAGTAATGATAGTCATAGTTTTTTATTAAGTTAACCAGCTGTTGAATCTTCAATTTCACATCCATCACTACTATAAATAAGAAAAATCCTGACTTAGTTGATACCAATCAGGATTTTACAAAGAAGTTTTAATTTGGAGCAGATAACGGGAATCGAACCCGCATCTTAACCTTGGCAAGGTTATGTTCTACCATTGAACTATATCTGCGTTGTTACTTTAATATTTAGTATATTTTAACTTGCATGGAATCTGAAAACCAAATTGCAATCCTGGATTATATTTTTAACCAAGTCAACCAGCCCAATCAACCAAAAATAGTTTGGTTCTCTGGGGAAGGGGAGGATGAGAAAATTAATTTTTTAATCCGCTTAAATGATTTTTTTAAACCAAAATTTGTTGAAAATACTAATGATAGTAGCTTTTTATTAAGTTTTAGAAACCATGTTGAAACTAAAAATTCAACGCCTTTAACCCAAGCTAACTTTGCTAATATTGCCAACAAATTACTAGCGGTTTTATTTGGTTCATTGCAATGAAAACAGTTAAATAAACCAACTGGAAACTGATTTCTTGTAATTTTATTTTTAGCTTTATTATGGTTAAGACAATGCTGACTAAAACTTCAGTTAACTAAGATAAGTAAATTTGTAAACCAAAAGGGCATTTTGAGTTTTATTAAACAACAATGGCCTATTCTAACAACATTGGTAACAGTAGGGACCACATTAGGTACTCCAGTATTTTCATTAACAATAGCCCAACAAGATGGTATTAAGCAAAATGCGGGGAATGATGTCTTTATCTTTTTGATTATCTTCTCTGTTTTTTCTATTAGTTTAGGGCTTGTTTCATCACTTATCTTTTTAGTCTCCAGCTTATTTTCAATTCGTCAGAAAAAAACGTTGGATGCTTTGGATAAAGTGTTATCAAAATTTATTGATAAATACTTTTTTTTAGATGAAAAAGAGATCAAAAAACAACTTAAATATCAGTTTAAAAACAACGGTGTTTGCTTTTTTTATGGTTTTGATTTTGATCAGGCAGAGTTTCTTGAACAATCAATGAACTTAATGTTGTTGTTAAAGCAAACCAATTGTTTTATTTTAGTTGGTTGTAAAGAAAGTGAAATGACGCTCATTAAAAACAAGATAGAACCTAACATTAACTTAAAACAAAACAGTTTTTATCTGGATTTAAGCAATGAAATTTCACAAGTAGAACAGATCAGCAAATTTAACTTGTTGTTTAGGCAATTGAGGCTCAGTTCTGAACTATTTTACTTAGAAGATTTTTTTGATTATCTGACTACTGCAAAACAGATAGTTAACTTTCTTTTTAAAACAAAACCTAACCTTGATCAATTTCAAGAAAATCAAAAGAAATTATTTGATTTTTTAGCGTTATGAGCATTAGTAATTGGTACTGATTTTGAGTTTAATAACGTGTTGTGATCATTTAATAACCACTTTGTAATTGATAACAGTTTTAAACAGGAATATGACAAACCCAACATTACTGCTTTCTTTAATCGTTCTTTGCAATTTTTTCAAGAAAATAGCTTGGTTTTAAAACCTGAATTGTTTAGTTTGCAAAAATACACAAAGGATGTTTATGGTTTAAATGTTATCAACCAACTAAACTTAAATAAGCACCCTATGCTAACTCCATTAACATGGGATAAAAAGCAAAAGTTTATTAGCTTTATTGAAAGCTGTGTTCAAAAATACAGTCAAGTTAAAAAAGATAACCAGGTATTTAGTTTAACAGTTGGTAAGCGCGTCTTTTTTCTATTATTAATAAATAAGCAGTTTAAACAAATTAAACTTGAAACAGCACTAAAGTACCTTGGCTTTAAAACTTCCCTTGGTGCAATGGACAGCACAACTGAGTCCTAATCAGTAAATAGAGGTTCAACTCCTCTAGGGAAGGCCAATTTATGATAAAAACTTAGTTAAATTGCTAGGTTTTTATCTAGTTTTAGGGGGTGTAGTTTAGTGGTAGAACAACAGTCTCCAAAACTGTCTGTGTGGGTTCGATTCCTTCCACCCCCGCCATTTACAGATGCACTTCAATTCAAACTTTAAAGAACGCTTTAATAAGATTGCAAAAAAAGTTAATTCACTGGACAGTGAATACTATGAGTTTTCTTCTTTTATTGAAAGGATTAGAACTACCTTTGGACTTTTAATTGCTTTAACTGTTTTAAGTAACCTAATCATTATTAGCTTTGTTTTAATTTGATTTTTTACTGATGGCTTTGGTCAGTTAAGGCTGTTATTCTTTACGCTTTTTATCCCCTTTTTTATCTCTTTATTAGTAGCAATCTTTCTAATCTTTCTTAACAATTCATTCCGTAATTTTTTTCAGATTAATGAAAAGAACTGGCTGTTCTTATGGACCTGTGTTTTTTCCTCATTACCAATCTTTAACTTGTGGTTAATTGTGAGATTAAATAAAACTATTAAGAATTTCGCTAGTGATTATGGTTTTAAGATTGTTAATAAATATAACAGTTTAACAAGCGGAATTTTTGTGTTTGACTTTGCTGATTATGTTAGTTTTGAAGCCAACCTGACTAATTGAAAAAACACAAACGATAAAAATCGTAATTTTGTTAACTTCTTTGAAACAATAAGCAAAGAAAAAACTGGTGTTGTTCAAAAACCAGTTCTTAACTTTCAAAGATTATATGTTAATCGTTTGTACTATCAAAGTAAGCTAAGTGTTGGTAGCAACCAACAGACTCCCCAAACTGCTTTTGATAACCTAAGAAACTATGTTGAAAACAAACAGCGTGAAACAGTAAGGGTTAAGCAGTATATCCTCTCTTAATTAAGTGAATATATTAATATTTATTGGTCTTTGTCAAAAAATAAGATGAAAAAGGCAATCCACTTTCAGAGTCAACCAGTTGTTTTTAACTGTGCTTCATGCAATAGCAACTTTACCATTGACTCCACTGCCAAACAAAAGGATCTTGCCATTGACATTTGTGGAAAATGTCATCCTTTTTACATAGGGCAATTAACCAAACAAACCGTGCATGGACGGGCTGAAAAACTTTCTCAAAAGTTCAACGCTGGAAAGGCTTTTTTAGAAAAAAAACTAAAAAGAGTAACCAAGCTAAAGTTGAAAAACAAACTAGGCACCGTTCTATTAACGAGCTTTAGTGGATTTTGACAAACAACTCTTTTTCAATGTTGAAAAGATTGTTGAACTTACTGAACAACTTGAAAAAGATCTCAATAAACCTAACCTTAGCTTTGAGCAGATTAAAGTTATTAACAAAGAGTTAAAACATAAACAACCTTTAATAGTTAAATTCAAGGAGTTGCAAAAGCTGGTTGAAAATGCTAATGAAGCTGAACAAATTCTTAACAATTCCAGCTTAAAAGAATTGCATGAAGAAGCTAAAAAAGAACTTGAAAAAATCAAAGCTAGTTTACCTAGTTTAGAAGAGGAGATTAAGTTCCTTTTACTACCGGTAGATGAAAATAACCAAAAGAATGTTATTGTAGAGATCCGTCCAGCTGCTGGTGGGGATGAATCTTGCATCTTCTTAAGTGATCTTTTTAATATGTACAAAAACTATTGTACTAGTAAAAATTGAACGGTTGAACTTAATGAAATTATCCCTGCAAGTGTGGGGATTAACTTTGTTTCTTTTGCGGTAAATGGTACTGATGTTTTTGCTAAACTCAAGTTTGAATCAGGAGTACATAGGGTGCAACGTGTTCCTTTAACAGAAGCTAAAGGTAGAGTGCATACCTCAACAGTTACTGTTGCTGTTTTACCTCAATTAGAAGAGGTAGAGATCACCATTAATCCTAGTGATTTGAGAATCGATACTTATCGTGCTTCAGGAGCGGGTGGTCAACATGTTAACAGAACTGAAAGTGCAGTTAGAATTACCCATCTACCTACGGGAATTGTTGTGGCTTGCCAAGAAGGTAAATCCCAGTTTTCTAACCGTGATAAAGCAATGAAAATGCTACGTGCTAAGTTATGGGAAAATGCTCAAAATAAACAACTCTCAACCCAAGCGGATTTAAGAAAAAGTCAAGTTGGTAGTGGAGAGAGGGCTGAGAAAATTCGTACCTACAACTATCCCCAAAACAGAATTACAGACCACAGGATTAAATTAACTATTAATAAGCTTAATACTGTTATTTTGGGAGATCTTGATGAGATCATTGAAGCTTTACAAGCTGATGAAAAGAAACAACAGTTGGAAAAGTTTATTAGTTAGATGACCCTGTATGAGTTTTTTTTAAATCAAAAGTTAGTTTACCAATCCAGTCCCCATTTTAACGGGGTATTTTTAACAATATTGGAACACTATGGTTTTCAATTTAAAACAATTGATAAACTCTGAAAAAGTAAGCTTCTAATTACTAGTGAGTTAACTGATAAAATCAAACAACAATTAAAGTGTTATTTTATTGAAAAGATCCCTTTGCCCTATTTGTTGGGAACAATTCAACTAAGGAAGCTTACTTTTAAAACTAAGAAAGGAGTTTTTATTCCTCGAATTGATAGCTTAGCACTAATTGCAAGTGTTAACTTAAAAAAATAAAAACTGCACTTGACCTTTGTTGTGGTTCAGGTACTTTAGCCATTGCTTTAAAAAAGAAGTGTGATACACTTGATGTTTATGGTAGTGATATTGATATCCAAGCATTAAAACTAGCGCAACAAAATGCATTAATTAATAACGTTAGTATTAATTGAATTGAAGCAGATTGATTTGATTGTTTTAACAAGATAAAAACTCCGATTGATTTAATTGTTACAAACCCACCTTATCTGAAAAAAACACAACTAAATAAAACATTAAATTATGAGCCTAAGCACAGCTTGGTTTTTCAAAATAAAAATAGTTATTTTGCATACAAGCAGTTGTTTAATCTATTACTAACAAAACGATCAATTAAACAGTTAATTTTTGAATGTTCTTTATTTCAAAAAGAAAGGCTATTAAATTTGTTTTCAATCTTTAAATCAAGGCCGATTTTTAACTTTCAAAAACAGTTTATTGGTATGAAAGTTGATAATCAAAAACTCCCAGTAGTTGATATTAAAAATACCAAAACTATTAAGCAACTTTTAAAAATGGGGCTAGCAGGAATTGTAAATACTGATACACAAATGGGATTAATTAGTTATTCAGAGTCTACTCTTGACAAAATTAAACAACGTGCACTTAACAAACATTATGTATCAATGTTTGGGTTAGAAGAATTAAAGAAGTTACCAAAAAAACTACAACAAATTGCTAGTTACTTTTGACCAGGTAGTTATACCTTTATTAAAAATAACAAGAGCTACAGGGTTCCTAAAAACTTGGGCTTATTAAACCTTTTTAATGCAATTGGTAGGGTTTTTTGTACTAGTGCTAATATCAGTAATCAAAAACCATACACCAAATTAAGTGATTATCAAAACGATAGTTACTGAATAAAGCAACCTTGTTTTATTATTAGAAGCACTTCTAAAGTGCAATCAAATAACACACCTTCACTTGTCTATAATTTAGATACAAAACAGTTGGTTCGCACCACAGCTAAACAAACAAAACAGTTTCATAAATTAATAACTAAACACCAGTTAGCTATCTAATACACAAACCAAAATGAAACCAAAGATAATAACAAGCTTTTCAATATAAAAATGCTTAATTCAGTTTTATTTTTGAAATAAAACTATTTTTTTTAATTAGTATATAGCATCCACTACTAAAACATAAGGGATGAAGCTCAAAGGTTTTTTAGCTGTTGGTGTTAGTGTTTTTGGTTTTTCTGGTTTACTGATGGCTTGTAGTGTTGTAAGTCAGTTTGATCAAGTGGATGATGGCAAAATTAAGCTAGCATCTTCATTAACTTCGAAACGCGCAGCGGAAGCTTTAGAGACAGTAGTTAAAAAATATAACGATACTAAAGATCCTGGTGATTATCCAATTGAAATAGTACAAATTGCTGGGGGTTATGATGGGGGTAAAAAGGATGTTCAAACCAAGGTTAGTACCAAAGATAAAAATAACTTTTATAACTTGATTTTAAACTATCCAGAAATAGTAAGTACCTTATCAAGGTCTAAAATGGCTTTGAATTTTGATGGGATTAATGTTGATAAACTCCATCCTAACTTTTTAAGCTTTAACAGCAGAATAGGTGGAATTAGAGATGATGGAATCTATGCTATTCCAATATCTATGTCCACTGATCTGATGGTCATTAATGGCCCTGTTTTACACTATATTCTAAACAGTGCTAGAAAAGAAGGTACACCAACTAGCACTACTGTTCAAGCAACTGTCAGTTCAAGAAGTGCAGAAAAAAAAGGTACATTAGAAATTGCAAATGATAGTGAAACTACTAAACTTTGACAGAACATCCAAACCACTGCTCAAAACAACAGTAATGAAACAACTAAGGAGCAAAAACAAGTAAAAAGATCTAGTAGTTCTTCATCTACAACATCTACTACTGGTGAAACTAAAGATACTACAAAATCAGATAACAAGATTAAAGAGTTTTGGGGTGAATATCAAGAAGTGGATGGAGGGTTAAAGAATTTTACCTTTAAAGCAAGCATCTTTGAAAACTGAAATGAAACGTTAGATTTTGCTACTAGAATAGCAAACTCTTTTCCTGAAAAGGTTAAAAATATAACAAATAAAACTGGGCTTGATTTACAAGGTGTTTTAGGAGTTGATAGTAGTTCTAATGCACTTTATGCAGCAGTTTTTGCAGCTGGTCAAGCTAACTATGATAACTTCTTTTTTAACATCGATAAAAGAACTGGTTATGCAGATTACTCTAACTTTTTAAATAAAGATAGTTCATACCAAAATTTAGAGAGTGTTTACAATGACTTTTATAAATTAATCCAAGCTAATGGTTTGTTTGTTAACCGTGGTGGTTCCTATTCATCCAACTTTGAAAAATTTCACCAATTGGCATTCTCAGTATCTTCTTCTGGAGGATACAGTTATTACTTTGCTAAAGATAATGCTAAGCGCTTAAAGTTTAGTAATTATGCTATTGAATATCCTAGTTTTACCCAAACAATTCAAGCTCCTAATTCTTCAGAAACAGAAAGTAATTTACTTGGTACTTTTAAATTAAGTGAAAAAGATATCAATCTATATAAAGGTTCAATTCCTAGTGGAAAACAACAAGGAGTTGATGCTATCTTAATTAGTAACCCAAACTTAATTAATATTCTTGAACAAGCAAAACAAAAAAACACTGCACAAGGAAGTGAATCAACCACTAACAAGATAATAGGTTATACCGCCACTGCAAATGTTAATGTTGATAATCAAAACATCTTTTCTGTTAGCAAACTTAACAACGAACAGTTTCAAAGAAAAATCATTGTTAATGCCACTGAAGAAACACTTGATCAATCCCAAACCTTACAGAGCAATGAATCAATTGTTTTACCAATGCCTGGTAAATACAAATCAACTGATAAAAATAAAGTAATGATCACCCAAGGTCCTAACTTAATAGGCATCCATGCAAATGAAAAAGAAAATATTGAAACTAAAAAATTTGTTAATTGGTTTTTAAATCAAAGTATTACAGATTGGAATAGTAATAATCAGCAAAAAAATAGTGATCAAACAACAAAAACTGCTGCTGAATATTTCACTGATCAAGCTTCTTACATCCTTCCTTTAAAGGAAAAATTTAACAAAAGTTCAGATTTAGAATTGAAAGGCAGTAGTAGTTCTTCTAATTTAACAACCAGTAGTGCTAGCGCCTCTTTGTTAATAAGTAATAATAGTTCAACTGCAAGTTCTCCTGCTCCTAAAAAAACAAACAATAATTCTAATACCTTTACAGCTAAAGCACTAGAATTATTCCAACAAGCTGCTAACAATGAAATTATTCCCTTTAGTGATCCAAGTGACTTTAGGAACGGCACATTCCGTAATAATATAAGCAGTAGTTTTAATGCTGCGGTGAATTCTAAGGTTAGTTTTAATCAATTTGTCCAAAACTTTATTAATAGTTTAGGATCTGGATTTAGAAGATAATTAGTTAACCTCCCCCAACCTCTATTTTGTGTTACTAGTGCCTAAGGTGGTAGTGGAGTACCACCAACTTGATAAGGTTGTCAAAGAGAGTTTGGAAGTGGAAGCAACTTCCAACTTTGATCCAACTAAAAGGTTGCAAAAAGAGAGTCCAAAAAAGGATTCAAGTAAACAAGCGGAGAAACTCACCGAAACAACTTCATCGATGAGTAGTGGTGGGGCTACATCCACTCGAGCAAGGCCCTCAAGGTTGAGGTGGAAAGGGGGAGTCAAAGTGATTCACTTTTAAAAAACGACTTTGCTAAAAAGCCACTGAAACATAAAGAAAATAGTGGGACAGATGTGAAGTTGGATGCGAGTGGGGAGTTTGCAGAAGACAAAGCCTGAAAACCGGTGTTGAAAACAGATGAGATAGCAAAGGAGAAGGGGATGGGGGCGACTTAGACTTTCTCCCCTGAATCGGCAATGGTAAACCCTTCTCCAACTCCCCCTCCCCTTCAACTTCTGCTTCCTCAACCCCACTCCCCACTTTTTCTAACATCAATGTTGGGGTTAAATCAATGATCACTCAACACTTAAATCAGCAAAACACCCGGTGGGTGTTTACCCCTAACTCTTCACCAGACATTTGAACTGGGGCAGGGTATCGCAAGGGTAATGACAACAATAACGGTATCTCCTTAACAAGTGTGTTGCCTAGTAGTAGTAGCAACACGTTTAATCCCAATTCAGATGATAATAAAGTCACACCATCAGGTGGCTCCCCCAAAACAACCACCTATCCTTTTTTACCCAACTCCATCAGTCCCACCAGTGACTGGATCAATGCATTGACTTTCACTAATAAGAACAATCCACAACGAAATCAGTTGTTGCTCAGAAGCTTACTAGGAACTATTCCGGTATTGATCAATAAGAGTGGAACGGGAGATGAGTTTAACCATACGAGTGACCAGAAGTGGGATAAAACTGAGACAAATGAGGGCAACCTCCCGGGGTTTGGGGAGGTGAATGGTGCTTCTTATAAGATTTTTACTTATTTAATAATTAAAAAAAGTGTTAGGTTTTTTAGTTTTTTATCTATTTAATATTTAAGAAATTCTCAAATTTTTCTTAGTTTTTTATTTGTTTAATAGTTAAAAAAAGCGTTATGTTTTATCTATTTTATTAGTTAAAAAAGTTTTGAATTTTTATCTATTTTTAGTTAATAAAAGTCTTATGTTTTTATCAAATTTTTATCTGTTTTTTGGTTAAAAAAGTTTTAGATTTTCTTTCTTAAATTTATTTATTAAATAGTTAATAAAAGTGTTAAGTTTTATCTATTTTTAATTAATAAAACCTCGACCCCTCTTCCTATCAAATCGCTGACCAAACCCTTTTTAACACCAACTTGTTTGTGTTGTTCAAGTCCAAGGATGTGAAGCTTACATATAGTTCAAGTGGCAGTAACAACATTAGTTTTGATTCAACTAGTCAAGGTGAAAAACCCTCCTATGTGGTCGAGTTTACTAATTCCACCAACATTGGCATCAAGTGAAGCGTGGTGAAAAAGTATCAGTTAGATCTACCAAATGTTACCAATGAGATGAACCAAGTGTTGCAAGAATTGATCCTTGAACAACCTTTGACTAAGTATACCTTAAACAGTAGTTTGGCCAAACAAAAGGGCAAAAGCCAGATAGAGGTGCATCTGGGTAGTGGGCAAGCAAATAATTGACAATCGATGCGTAACTCCATCAGTCTAAACAACAATCCCAGCCCCAATGCATCAACTGGGTTTAAATTAGATAAAGGCAATGCGTATAGAAAACTAAGTGAATCCTGACCAATTTACCAACCAATTGATGGGACCAAGCAGGGCAAAGGGAAGGATAGTAGTGGGTGGAATAGTGAAGAAAATACGGCAGCAGGGGATGCGCCCCTTGTTACAACGAGGGAGGTGGTGTCATCAAAAAATAAATTCACCAAGTACCTCAACACCAAGCAAGCATTGGAAAGGATCGGCATCTTGTTTGATGGGGATGGAATGAGGAATGTGGTTAGCCCAGCTCTACCAACCCAACAAGGTGAAAAGTGGTCAATACCAAACCACCAACACCTACAACAAGTTGATTGAGCCTGACAAGTGAGAATCATCAAGTGATTTGAACAATATGACCAACTTGTTAAAGTTACTGACAACTAAAAATGTGAAAGCAAAGTTGGGGAAGGGAACAGCTTCTTCGCAGGGAAATAGTGGAGGGGGTGTTAGTCAAACGATTAATACCATCGCTACTACGGGGAATATTAGTGGCAATGGAACCATTCAAACGGCTTATCCGGTGAAAAAAGATGAAGCTTCAAATGTAGCGATCAATTCCTTGATTAACGCTACGCCCTTGAATAGTTATGGGGATTTAAATAATGCTAGTTTTTCTAAATAATTAAATTGTTAATAACAAAAAAATCTCTATTAAAAAAACCAACTTTAAAGTTGGTTTGAAATTCTAAATGGCGCGCCCAATAGGACTTGAACCCATAACCTTCTGGTCCGAAGCCAGACACTCTATCCGATTGAGCTATGGGCGCATATATTAATAAATTTTATTAATATAAGCAAGAACAATCTAATTCTTTATTAGAATTTAAACGATTTCATCTAATAGTTCAAATCACATATGGCAAAAGATAAAAAAAATAAGGTTGATGGAACAGAGCAATCAGTTGATCTATTTGAACGTACAAAACTTGAAGATACACAAGTTTTAAATGAAGTTGAACTTGATGATATTAAAAAGATAACAGAGCTTAGAAAAGAACTTGAACATACTTTTGAACCACAAACAAGAATGCAAATTAAGCGTGAAATTAAAGAAATAGAACGCAAAATGAAACGTTCTAGTCGCTAATTGATGTTTGTTAATTTACATACAAATTCATACTATAACTTTCTCAATTCTGCCCTTTCTCCTAAAAAGCTAGTTAATCTAGCAATTAATGATCAGCAAAAAGCTGTTGCTATTACAGATCCTAATCTTTTTGGCGCTGTTGAATTTTTTATAACTTGTAAGCAAAATAATATTAAACCAATTATTGGTTTAAACTTAACTGTTGAATACCAAAAAAATGATGTTAAGTTATTACTAATTGCTAAATCAAATAAAGGCTTTCAAACGTTGAACAAAATAGCATTAATTCAACAAAAACTTGAAATTAATTCTTTAGTTGATCAACTAACAGATATTGCAGTAATTATCTGTTCTTTAACAACATGAAAATCTACTTATAAGGATGTTTATCAAGCAAAAGGAATTGAAATAAATCAAACCCCGATTGCCATTCTTGCAAATGCTGTTAACTGTGAAAAAACTAATAGCGATCAAGTAGTTTTAACAGTTTTGAAACAAATGAAACAAAACCAAACGGGAAAAATAACTACATTTGATTGGGATTTTAAACAAAAATTAAATCAAATTTCAATTAATGAAAATTTAAAAGTAAAGAGTGAAATTCAACCTTTTTTAGATCAAAAAACTGCACAACAATTATTCAGTGAAACAGAACTTAATAATCTGAATGATCTAGTTAATAGATGTGAATTAGATTTGGAGCACCTAAAAGCTGCTTCACTTTCTTTAACTGATAATGATGCAGCAGTTTTAGAAAGTTTGTGCCAAACCAATTTAAAACAGTTTTTAGATAAAAATCAAGATCTAAATAAAAAAGCCTATCGGCTACGTTTAGAGAAGGAATTAAATGTTATCAATAAACTTAATTTTGCTAGCTATTTTTTAGTTGTCAATGATCTTGTTAATTATGCTTTTAAAAAGGACATCTTAATTGGTTCTGGTAGAGGTTCTGCAGTAGGATCATTAGTGGCTTTTTTATTAAACATTACCAAGATAGACCCAGTCCAACACCAGCTTATTTTCGAACGTTTTATCTCAACCCACCGTCAAGATCTACCTGATATTGATATTGATATCATGGAGAATAAAAGAGCAGAAATGATAAATTATCTGTTTGAAAAATATGGCAAAGAAAACTGTGCACAAATTGTTACTTTTCAACGTTTTAAAACCCGTTCTGCTGTTAAAGAAGTTGCTAAATTATTTAATGATTATGGCATTAGTGACATGATCCTAGGAGTGTTACCTAAAGATCAAACTATAACATTCACTGAGCTTAAAGCTACTGAAGATAGTGCTTTACAACTTTGTTTACAACAGTTTGGTTTAATTGTTGAATTAGCACTAGCAATAGTTGATTTTCCAAGACAATCAAGTATCCATGCTTCAGGCATAGTTATCGCTTCAAATTCTTTGATTAAAACCATTCCCTTGTTACAGCTTGACAATAATCACTTTTTAACTCAAGTTTCAATGGAATGGTTAAGTTTTTTTAATCTCAATAAGTTTGATCTGCTTGGTTTAATTAACCTTACTATGATTAGCGATGTAATTACCCAAATTAAACCATCTAACCAGACCGTTAACCAGTTTTTAAATACCATTTCTTGAACTGATCAAAACACCTTTATAAACTTAGTAAATGAAGATACACTAGGAATCTTTCAACTTGAATCGTTTGGCATGAAAAAATTACTGGTTCAGATTAAACCTAAAACCATTAATCAACTAGCAATTGTTCTAGCGCTTTACAGACCAGGTGCACAGGATAACATTAACCTTTTTATTAACCGTTTGCACAATGGTTATGATCAATCTGACATTGATCCTAGGATTTTACCCATTGTGAAAAATACCTATGGAGTTTTAATTTTTCAAGAGCAGATCATTAACATCGTTAAAGTTGTGGCTAACTACTCTTTAGAAGAAGCAGATAGCTTCCGTAGAGCCATTTCTAAAAAGGATGTTAAATTGATCCAAAAAAATAAGCGTAACTTCTTTGAAAGAGCAGTTCAAAATAACTTTGATTTAAAGACTACTACCAAAATTTTTAGCTACATAGAACGCTTTGCTAACTATGGGTTTAACCTTTCTCATGCGTTGGGTTATGCACTGCTTTCATACTGAACAGCTTGACTTAAAACTAACTATCCTGTTTATTTTTATTTATGGTTATTAAACCATTTTCAATCTAGTAAAGACAAACAAAAACTAATTATTAGAACTTTAGAAAAAAGTGGTATTGAAATTTATCCACCTCTTTTAAATAAAGCTCAACCAAATAGTGTTATAGAAAATAAAAAAATTTATTTAGGTCTAAACCTAATTAAGGGAATTAATGACAGGTACATCCAAAACTTACAAAAAGTGCAACATTTAATTCAAACTCAAAATAACTTACAACTAACTGATGTAGTAAGTTGGTGTTTGGATAAAACCATTGGTGATATCCCTTTAAAAGATTTACTTTTATTAAAAACTATGGGCTGTTTTGATTTTTTTGAATACACTTATGACTTTAATGATGCAAAGGATTTTTGAATTAAAAGCGATCACCTATTGTTTACCAGAATGCCTTTAGAAAAAAAGGATAGTAATTTTTGAATTAAACAATTTTTTACCAATTAGTTGAACATGAAAAAAGCAATCCTGATTGATGGCAATTCCTTAGCATACCGTGCTTATTTTGCAACGTGAAAACAAGTTGAATATGCTAAGCAAAATAATTTACCTTTCAATAATGCAATAAGAACAATGTTACTAATGTGTTGGAATTTAATTAAAGCCAATGTTTATCAATATGGAATTGTAAGTTTTGACACTAAAGCGCCAACTTTCCGTGATCAAATCTATGAAGGATATAAACAAAAAAGGGTTAAAACTCCAGTTGAACTTTTAGTACAAATTCCTCTAATTAAACAAGCGCTTGTTTATTTAGGGTTTTTAGTTTGTGAAAAAGATGGCTTTGAAGCAGATGATTTGATTGGTAGTTATGCCAATTTATTTACAAAGCAAGAAATAACAGTTGATATTTACAGTTCAGATCGAGATATGTTGCAATTAGTAAACGCCTTTACTAATGTGTTTCTCTGTGTTAAAGGTACAAAGGAGATGGTTATGTACAACAATGAAAATTTCAAATCACTTTTTTATGGTTTGGCGCCCTATCAAGTTGTTGAATATAAGGGGTTAGTTGGTGATAACAGCGATAATTTAGCAGGGATTAAAGGGATAGGTCCCATCAAAGGGATAGAATTACTCCAACAATATGGAACCATTGATAACATCTACACTAACTTCAATAATCTCCCCAACCAACTTCAAAAACTTTTAAATAACCAAAAGGAAATAGCTAAAACCTTTAGTTTTCTAGCTAAAATTAAAACTGATATTGAACTTGATCAAAACATAGATCTTACTGGTTTAAAACCAATCCAAAAACAAGCGTTAATTCAACTTCTAAGTGAAAACAAAATTAATACTTTAGTTGAAAAATTTTCAAAAATATAATGCCTGAACTTCCTGAAGTAACTACTGTTATTAATGAACTTAAAGAAACTGTTTTAAATAAACCTTTAGATCAAGTTCAAGTTAACCTAAGAAAGGTTTTGAAAAATATTGATCCTCAATTGCTGAATAAACAATTAAAAAATCAGTTTTTTACTGATATTAAGCGTAAGGGTAAATATATCATTTTTCTTTTAAGTAATGGTTTGTATTTAGTTTCGCATTTACGTATGGAAGGTAAATACTTTTTTGAAGAAAGAGGTAGTAAATTTAATCAAAAGCATGTTTTAGTAGAATTTCATTTTGATGATGGTAGTCAACTCAATTATCATGACACCAGACAATTTGGAACGTTCCATTTGTATGAAAAGTTAGAACAAGCAGCACAATTAAATAAACTTGCATTTGATCCTCTAGAAGCTGGTTTTGACTATAGGAAAATCTTCCAAAAAGCACAAAATTCAAAACGTAAAGTTAAAACTTTTATTTTAGACCAAACAGTGATTAGTGGAATTGGCAATATTTATGCAGATGAAATCTTATTTGCAAGCAAAATTAATCCTGAAACAATGGTTGATCAACTAACAATTAAAGAGATAGAGATTTTATGTAAAAATGCTACCAAAATTTTAGCTAAAGCAATAGTTATGAAAGGTACTACCATCAGCAGCTTTAGTTTTAAAAAAGATCATACTGGAGGCTATCAAAACTTTTTAAAAGTTCACACTAAAAAAGATCAACCTTGCTCAGTTTGTAACCAATTAATTGTTAAAAAGAAGATTAATGGAAGGGGGAGCTATTTTTGTTTAAACTGTCAAAAAATCACAACCAAAGTTTCTACAAAACTCAATCCATAAATTTTTTAAAACTTTAAATATCTTAAAATTAACTTTTAACAAACTAATTGAAAACTTTATTTAACAAAGTAATGCTGTAATTAATCAATTAGTTAATGAACATTAAATAACATTTATGAAAAACGAAATTAAATATCTTTATTCTGACTTGGATGGTACTATTGTTAGCTGAAATCCTAAAACAGAATTTGTTTATCAAAATAAAAGTTATAAAAATTTCCATGAAGTTAGTGATGCTACTATTAGTGCTTTTTACCGATTGCAACAAAAGGGAATTAAGGTTGGTATTGTTACTGGTAGGGATTATTGTCGGGTGTTATGACTTGAAAAACAACTTAGAACAGGATTGCCTACCATTACTTTAGATGGGGCTATTATCTTTTATCAAAACGAAATCTTAAGTCAAACTTATTTAGATGATAGATTTATTGAAGGGATTAATAACATAGTAAAGCGCTTTCCTGAAGCTGCTTATAAACTTAACAGTGGTTGAATTAGTTACTTTACTAAAAACCCTTCTGTTATCTTTGAAATTGATTATGCTTTTCTTGGCTATTTCAACCCTAACACCAAACTACAAAAAAAGTTTATAGACAGTACTGAAAATTGAGATCTTAATAAACTAAAAGTTAATCAGGTTTACTTTGATATTGATACTTGCCCCTTAGCAATGCAAAAGGAAATAATTGAACTAATTAGTGTTAGTGATGTAAATGCCAAAATCTATGAGCACTCTATGTACATTATTAAAAATGGTGTTTCTAAAGCTAGTGCATTGCAAAGCCTTAACCAGTTTGCAATTCCAATAACAAAAGATAACACTATTGTTTGTGGGGATGGAGATAATGACATTGAAATGATGCAGTGAGCTAAACACAGTGTCTCACTAATCGGTAGTAATCCCAAATGCTTTGCTCTAGCAAAATACCACACTGATAGTGTTGACAATGATGGTATTGCTAACTGGATTGAAAAAAACTTGTTATGTTAATTGCAATCGTAGGTAAACCAGGTGTTGGTAAAACCAGTCTATTGCAATATCTCAAAGATAACTATCACTTTTCAGTTTTTTATGCAGATAGTTTTATCCATGAACAGTACCAAAAAAACAATCCAGGTTATCAATTAATCATGGATCATTTTGGCAAAGAGTTTGTCAATCAAACTGAAGTTGATCGTAAAAAACTAGCAAACTATGTTTTTAGTGATGATAAGTTAATCGAAAAACTTTCACTAGTAACAAAACCGCTGTTAATAGCGTGAATCAAATCCTTAAAAACCCAGTTTCAAAAAAAGCTAGCACTAATAGAGATTGCTGTGATGCTTAACTATTGAAATGAATATAGATCGTTGTTTGATTATGTGATTAAATTAGAAAGGGATGATCAGCTAGTTAACTTAGCTTTACAACAACGTAATAGTCATAAAAAAGTTAAGGATTTGATTAAAGAGCCTAATTGCAAAATAGATACAATTTTCAACAACGATTCGATTGCAACAGCTGCTTTAAAGCTAATTAAGTTGCTAGAAACTTTTTTAGAAAGAAATAAATGCCGTTGTGATTGTTGTCATATTCAGTAATAAACTTAGCATTTTTTTTAGCTAAATCAGTGCCATTAACCAAAGCAACTGGCCATTTAACACTTTGAAATATTTCAACATCATTATCAGCATCACCAAAGACCATAGTCTTTTCAGGATCAAGATTATAATTATCAACTAATACTTTCAATCCATAAGCCTTATTAACATCTTTTTGCATGATATCAAGAGCAAATGTCATTGAACTCACATAGTTAATATCTTGAATTTGATCTAGTTGTTTTGCTAGAAAAGGAACTTTTTCTCTGTTTTTAGTTACCAGTAAGATCTTTGTAATAGTATGTTGCTTAAAATCAAAATCCAAAGCTTTTTCAAACTTGTTAACTATGTAGTTATTTTCAATGAGATCAATTCCTACTATTTGACTTCTAGTTGAAAGAATTTTAGCTGAATCAAGCTCATGTGTAAAAGCAAAAACCTTTTTATCTGTATAGAAATAAAATGTTTCTTGAAATTCCAACAACATTTGCATAATTGCAGGTATTACTTTATTATCAATAGGTTTGATATGAACTGGTTTTTCACTAGCAAAATCATAAACAAGTGCACCATTGGAAGAAATTACAGGTAGATTTGGTGTAATAAAGGTAGCTGTGTTTCTAATTAAAATATGGCTTCTACCACTAGCAAAAGTGATTCTAATCCCTTTTTTCTGTAAATCCTTTAAAAACTCAACTGTTTGTTCTAATGGAATTTGGTTGCTTGAAAGCAAGGTACCATCAAGGTCAAAAATAATGTTTTTCAGTTCCATTTAGCTATATACTTTTATCTATTTTTTTAAAAGAACATTAATAATTCGATCCTGAACATAAACTACCCTCTCAATCGGTTGGTTGAGAATGTCATTTATCTCTTTATCCTGTTTAAATGATTCTAAAACATCTATCTCTAAACTACCTTTAGTAAATTCTTTAGCTGCTTTAAATTTGCCATTAATTGAAAGAATAACCTTAGTTTTAGCAGTCTCAAAAAGTTGATAATCAACTAAAGAAATCGCTTGTTTAACAACAAATGGTTCAAGTCCACATTTTTCATTCAATTCTTCAGCAAGAAAGGGCGCAAAAAACGACAGTACTGTTAAAAATCCCTTTGCATAATTTAAGCTAATTTTTTTGGTTTTATAGAGAAAGTTTAAAAAGATCATCATTTCACTAATCACCAGATTTAGTTCATGTTTGTCAAGATGACAATAACTGTTTTTTAAAAACAAATTGTAAGCAAAGGTTGTCTCTTGACTAACTTGATCAGTAACAACAGCATGATTAAAAAAGAAGTTATAAACTCGATCCAATCACCTTCTCATCCCGTTTAACCCTTCATCATTTCAAGTTAAACTAGCACTAATTGGGCCCATAAACATTAAGTACAACCTTAAAGCATCTGCTCCATGTGAATCAACAAGTGGTGTGGGGTTAATGGTATTACCTTTGGATTTGGACATCTTTTTACCATCAGGACCTAACACCATACCCTGATTAATTAATTTTTGAAATGGTTCTTTTGTTGATACTAGCTTCTTGTCAAACAAAAATTTGTGTCAAAAACGGGCATACAAAAGGTGTAAAACTGCATGTTCCGCACCCCCAACATAAAGATCAACTGGAAGGTATTGATCAAATAATTTCTTCGCTTCTTTTGAATCAATTGGTCAAAAATTAGGGTTTTTAATCAACATTAAATAACCCAGATAATACCAACAAGAACCAGCTCATTGGGGCATGGTATTAGTTTCCCTTTGGTAATGGATGTTATCTTTGACTATGTTTACCCAAGCTTGATTTCTCATTAGTGGAGAATTACCACTTCCATCTGGTTTGTAATTCTCAAGTAAGGGTAATTCAACAGGGAGTTGTTCTACCAAATGAGGAGTGTTATTTTCATCAAAAATAATTGGAAAAGGTTCGCCCCAATAACGCTGTCTACTGAAGATCCAATCACGCAGTTTATATACAGTTGTTAATTTCGCTTTATTTTGTGAAATTAACTCATTAGTAATAGCTACTTGTGCTTCTTTAGTTGTTAATCCGTTATATGCAAATGAATTTTGCAGCCGTTCTTTTTTATCAATGACGTTGATAATTTTCAACTTTTGTTTACGTGCGAAGAAGTTATCATTTTCATTGTGTGCTCCAACACCCATAATAGCATCTGTTCCATATCCTTCAATTACATAGTTAGCAACATAGACAGGGATCAATTCATTTGTAAGAGGGTGAATAGCATTTGTTAATAAATCTATCCCATCATATAAAGTTGCTTTTTGCTTTAAAGTTGTGGTTTTCTGGAGTTGTTTTTTTAAAAAACTAGCTACTTTTTTATTTGTTTCTGCTATCTTTTTTGCTAACCAATGGTTGGTTGAAACTGCAAGAAAACTAACCCCAAAAATTGTTTGTGGTTTAGTTGTAAAAATTGCAATAGCTTCCTTATGATCTTTTAGTTGAAAGTTAATAGTAACACCTTTACTTTTACCAATTCAGTTCCGTTGCATCTCTTTAATTGGTTCAGGTCAATCAAGTGTATCTAAGCCTTCAAGAAGTGCATCAGCAAAAGTAGTGATTTTCAAAACTCACTGTTTCATCTTGCGTTTTTCAACTGGAAATGAACCCCTTTCACTAACTGCGTTGCCATTACTATCAATTAAAACTTCTTCATTAGCCAATACAGTACCTAGCTGTTCACACCAATTAACATCAATATCAACTAATTCCGCTAGGTTTGCTTTAAACAGCTCACTGAAGATCCATTGTGTGTATTTGTAATAACGTGGATCAGTTGTTTTGAGACTTAAATGATAGTCATAATCAAAACCAAAACTAGTTAATTGATTAATAAAGTTATTAATGTTTTGATCTGTTCAACTGCCAGGGTTTTGATTAGAGTTAATAGCATACTGTTCAGCAGGTAAACCAAAAGCATCAAAACCAATCGGATGGATCACATTAAATCCTTTAGCTTTGTAATACCTACTTATTACATCAGTGATAGTATAAGCTCTAACATGTCCTAAATGTAATCCTGCTGCTGAGGGATAGGGGAACATGTCAAGGACATAATATTTCTTTTTGTTACTATCGCTTTCAAAGCGGTTAACATCTTTGTTTTTTCATTTTTTTAACCACTTTTCTTCAATTAAATTGTGATTGTACATCTAATTGCTTTGATAAGAACGCTTGATTGCATATTTAGTTAAGCGATAGAGTATGTCAACAATAATCGCAATAATCAAACCAAATAACAACCCAAAGCCAACAGGGATAAAAAAGTGAACCATTGTTTTAAATAAGACTGGTTGGTTTGCAACTGTGGCATTAAACCAACTGTTAATGTTGGGAAAAATCGTTAAAATAATCCCACTAGCATCACTGGAAGCGAGTAAATCAGCCCACTGAGCTGTTTGGTATGTATTAAGTCAAAAACTACCAATAAAAATAAAAAACCCAATTACCATCAAAAAAACTAGGATTGCAATTTTCACTAGTTTAAACAAAAGTGTCATTGTAGTTTTCAGTAAGTTATTTCAATTATAAAGTTAGATAAATTTTCAATCTAACTTTTGTAACTTTAATTTTTCAAGGATCATTTTAATAATAGTTACTATTGGTAGTTTTGCATCTATAATACAGTAATTAATCCCAAAATCATTACAAACCGCTTCCATAAATCCAGTGTAAACCCTGTGTAAATTTTGAAAGTAAAGTTGATTTTTAGTAAAGTTATCAATCTCTACTTTGCGGTTACGCATAAATAGTCTTTTTTCAAATAATTTTCAATCCCCATCCAAAATGACATATAAATTAGGAACCCCGTGCTTATTAACTAGTTCTTTTGCTAATCTATTTCACAGTTGGTTATAGTATGAAAAGACTGCAGGACGAATGATGTTGTGCTTAGCAAACAACCAGTCTTCAAAGATAGAACGATCAAAGATGGTTGGATTGATAGTGTTGCAATTGGTTTGGTATTTACCAAAGCGATTAAGCGTAAAATAAAGCTGAAACAAAGGTGAATAGAGCAATTCATCACTACGTTCATACATCTTTGCTAGTAAAAGTTCAACCAACTGGTCATTAGTTTCCAATTCACAAACAACTTTTGCAGCTTGAATGTGGTTAGCTAATGTATTAGCAATGGTGGTTTTTCCTAAAGCAATCATTCCCCCGATCACAATACAATTAGCAATTTTATTTGGTTGAAAATGGGGCTTTTTTAGTTGCATATAAACACTTTTTAATATGTTGGGTTAAGGTTTATTTTTAATAAAAAACCATCACACTCACTGACAAGTCTTAATGCTGCTCCATTTCTGGCCTGACATGATTCGACTCTAATGATGTGATGGTTCTAAATTCTAACAATTATTTAAATCTTAATCAATTAATTTACTAACCCTGTTAATAGAATTGGATTTGGGTTGTGTAGTGCCTCTAGGCAATGAACTTGAATTTGATTTTTTAGATGGTTTGATCGGTTCATTCTCTTGATCTAATTGCTGTTGTGACGCTGGAGTTTCTTGTTGATAATTTGTTGGCGTTGAATGATCAAGTTTAGTAATTTCAGCTATCTTTTTTGTTAGTTTTGCTTTGGTTTTTTCAAACTGCTTAATGAAGTTAGTAAAGCCAGTCTTTGGATTCTTAGTTTCATCAAAAATGGTTGCAATAATCTCATCAAGCTTGGTGAAATCAATTTCTATTTCGTTGGTGAGTTTTTGGTGTTTTTGAAGCGAATTTACAACTAACTTATATGCTTTTTTTAATGCTTGCTTTTCTTTTTCAGCGGTTTTTAAAAGTGATTGGGTTTCACTAATTTCCCCATCTTTAAGTGCTAACTTGTCTTCAAATACGGTTTGGATGTGTTGATCACGTTTATAAATTGCTTCATTACACTGCACTTCATATTCCTTTAACAATTCCTCTACATTAATACTTTCATGTTCTTCAATTAGTTTCTTATTGGCTTCTTTTATTGCATGTAACTGTTTATTTAATGCTTCAATTTGGTGTTCTTTTGCTTCTACCAGCTGCTGCATTTGGATGATTTCACTTTCACTTTGTTTGAGTTGTGAGAAAAGTTCGCTGTTCTGTTGAACTTGACGGTTAATCTCTCCTTCCAAGCGTACCTTAAGTTGTTCTTGCAAACTTAAGACCCTTTTTAAACGTTCTTTTTCACTTCTCGCTTCAGGACCAAACTTTTTTAATAACAAAACAAGCATCTGATTCCAATCAACATTTTTTGGGTTGCTTGTTCGTAGTTTGACATAAGCAGTTTTTGCTTGTTCTAAAAAAACATCAAGCTGGGCTTTGGAAGCAAAGGGAAACTTGGCTAAAAAGAAGTTAATAGTTTCTCTTCTTTGAAAGTCATCAAAGTCTCTATAGTAATCAAAATTATTCATGGAAAGCTAATTCTCTAAAGAAGCTAATGATAGCATCAGGATCATTAAAGTCTAAATCATAATGGTGTCTAACATAAACACTAACAAAACTAAAGATTGCTAAAACAAAACTAGTAAAGAGAAAAAACGGGAGTTTATTGCCATGGTTTTTAACAAACAAATCTAACTTTTCATTAGCAATTTTTTTAAAACTAACTACATCACTAAACTGGTTAGGATCTAGTAAATAGTTGTTAAGATCACTAACTTTACTTTCAACCCATTCCAAGTTAGCTTGGTTATATTGAAATTCACTTTTAATAGTTGCAAAATCAGTTTTTTCAACCTTGTTTAAAATTGATTTGAAGCCATTTGTGTATTTTTGAACAAAACTATCCATTTTACTTGTTATCAAATATTACTTCTAAGAGTTGCTCAGGTTTTAAACTGCCAAAGTAATCACTAAAATGATCAAGTTCATTGAAGAGTTTTTCAAAGGTTTTATAATCGTACTTCTGACCAATTAGTTTTTTTGTTACTGGGGTGATATCAACAACACTTAAAAAGTCCCCATAAAACTTAATATCAACAACTGTTCCTTTCTCTACTTGAACATTGCACTCAAATAAACCAGCATTATTAAAATAACGCTTGTTTTTAAAGTTGTATTCATAAGTTTTACCAAAGTTTCACTCCCATGATTGAAAGTGTTCTTTTGCTCTTTTTTCAACCTTTGCTAGTGCATCTTTAGTTAAAACAATTGTTTCTGCTTTTTCAGTAACAGTGAAAAAATTAATCATCTCTTCTAAAAATTTTGCTGTTGTTCAATTTGGTAAATACTCCTTTACATTAACAACGCGCTTAGCAACACTGTCAACACCCTTACTTGCTATCTTGGTCTTATCAACATTTAAATACTTTGCTAACTTAGAAAAGTCAGTGTCAAACAATAATGTTCCATGGACTAATAACCTGTCTTTAGCGATATATTCAGCTAACCCAGAAAACTTCTTGTTATCAATCTCAAGGTCATTACGACCATGAAATACAGCAGGTACATTTAAGCTATTTAAGAACTTCACCACATTTCTTGTAGTTTGTTCATAAGCATTTTCCATCACTTTACCTGTTCTTGGCAAAATAATAGAAAAACAGATGTTACCAAGGTCATGAAACACCGCTCCCCCGCCTGAAAAACGTCTAAACAAGTTAACCTTATCACTTTCCAACTCCTTTAAGTTAACCTCAGCATAAGTATTTTGGTTTCTTCCCACCACAATAGTGTTAGCGTTCTGCCAAAAGTAGATGACCTTAACTAACTCATTTTTTCTAAATTCAGTTAGCAATCACTCCTCTAAAGCTGCATTAAAATACGGATTGAAAACAGGGGAAGTAATAATGAAAGTTTGCATCAACTACCAATTAACTAAAGTAATCAAAGATCGCTTTTTTACAAACATCAGTTACCATTTCATTCATGGTAGGATGGGGTGAGATAGAATTGGCAATATCAAACACAGTGAGGTTGTTTTCCATCACCAAAGCAAGCTCAGCGATAATATCACTAGCAGTGCTAGCAATAATACATCCACCTAAGATAGCACCAGTTTTAGGATTAAACATCATCTTGACAAAGCCATTGGTTTCATGATCTGCAATTGCTTTACCACTATAAATAAATGGCAAAGAAGATTTGACATAATCAATCTTTTCTTTTTGCAATTCCATCTCACTATAACCTACAAAAGCAACTTCAGGATTTGTGTAAATACAAGCAGGACACTTGTTTTTTTCAGCAGGCTTTACCTGGTTTTGGTTCAAAATTTGATCAACAGCATATCTGCCCTGTTGGTAAGCGTAGTGTGCCAACATCATTTGCGTGTTAACATCACCTATTAGATAGATGTTTGTAGTTGATGTTTGTAGTTTTTCATTTAAAACAATTTTGTTGTTATGGTCACGTTTTAAATCAAGTTGATCTAAACACTCTGTGTTAGCAATTCTTCCTATAGAAACTAAGATTTTATCACCAATTACAGACTGTTCAACTCCATTAACTGTGTAAAACAGTTGGTTGTTTTCAGCTCTAACAACATGAGCATTGGTAATAATCTGAACTCCTTTGTTTTTAAGGTTTTACTTATCAGTTCAGAAACATCACTATCACAAACCTCCAAAATCCTATCAACACCTTGGATAATGGTCACTTCACTCCCTAATGAAGCAAATAAAAAAGCAAACTCAACCCCAATCACACCTCCCCCAACTACAACAAACTTCTTAGGTACTCCCTCTAAAGCCAAAGCTTGGGTTGAGTCAATGATAAACCCAGCTTGTTGTGCTTTTTCAAACCCTGGTAAAGTTAAGTATCTTGGTCTTGATCCGGTTGCAACAATAATGTTGTTAGTGGTGTAAGTTGTGTTGTTTACTTGCACCTTGTTTTTATCTATAACAGTAGCTTCACCTTCAATACTTTCTACCTTAGCACCCTTAATAATTGTTTTTACCCCTGCAACTAATTTATCAACTACTTCCTGTTTTTGTTTTAACAGTTGTTTTCAATCAAGTTTAGCTTGACCATTAATAGTGATACCATAATCTTTGGCATGAACTAAATAATCAATAATCTTTGCTCTTTTTAACAACGTTTTAGTTGGGATACACCCAACATTTAAACACACCCCACCAAAGTATTGCTTTTCAATCACTAGGGTTTTAAGTTTATGTTTGCCAGCATACTCCGCAGCAATATAACCAGCAGGGCCAGCACCCAAAATAATTAGATCATAATCCATAAATTAAGCTACTGTAAGATCAATTAATTCCTCAATTTGTTTTGCAATCTCCTTACCAAACCTACCAACATCCGCCCCATCAACCCAGCGGTGGTCTGCAGCTATTGTTAAAGGTAAGATGGTATGAACTGCAATTCCATTTTTCACTTTAACAATGCGTTCTTCTAAATTACCAGTAGCAACAATACACATCTCAGGGTACTTAATAATAGGTGTACCTACAGCTGCTCCTAATGAACCGAAGTTAGTAACTGAAATAGTACCTTTATTCAAATCAGTCAACTTAATCTTTTTTGTTCTAGCTTTGTTAGCTAAATCAACAATTGCTTGGGCAATTTCAACCACAGATTTGGTTTGGGCTTGCTTAATGTTAGGGACAATTAAACCTTCTTCAGTATCAACAGCAATTCCTACATTAATGTCATCATTTAAAACAATTTCGTTTTGATCAGGATCATAACTAGCGTTAAAAACAGGGAACTTCTTAAGCGCATTAACAATTGCTTTAACAAAGAAAGCAAAGTAAGAAATTTTCATGGAATACTTACTTAAAGCATAACCATTAACACTTTCACGATATTGTTTTAACTTGGTTGCATTAACATAAAAAGTTAATACAGTGGTTGGGATAATTGCGTGCGACTTTGTCATTGCTTCTGCAATCGCTTTGCGCATTGTTGAGATAGCAATGGTTTTCTGTTCAGTTTTACTTTCTGGTTTTTGGGTTGTTTCTACAGTAATGTTAGTACTACTTGCAACTTTAGTGTCTTTAACAGCTGGAGTTGCATGAGGTTTTACTCCAAAGATAGGAAAGAGGTTATCAGAAACCTTAATTTCCCCTACTACTGAAGCCCCCGCTTCTTTTACCTTAGTTACTTCTTCAGTTGGTTGAGGTTTTGGTTCAACAATTGGTGTACTAGTCTTTTCACCAATAACTGCCATCACCTGACCAATGCTAACAACATCACCAACTTTAACATTAATAGCGCTAATTGTACCTGCAAAAGGAGAAGGTAATTCGGTTGTAACTTTATCAGTTTCAACAACAAATAAAGCTTCATCTATCTTGATCTGATCACCAACTTGTTTTAAGATTTCAGTTACTTTTCCTTCATGTAAACCCTCACCAACATCAGTGAATTTAAACTCATTTGCCATATCGTATTGATAGTACTTAAAGTTTAAATTTAATAGTAGTGATCTATAACAAATAAAAAAAGCCAGTTTAGTTACTGGCTTTTTAGTTGTTAGACAAAAATTTTGTCTTACTTTTTACAGCAGCTGTTGCACTTAGCGCATTCGCACTTGCATTGGCAGCATTTCTTGTTGTCTTCCATCACTTTAATTTATGAAATGAGGTACCTAGCTCTAAAAAAATAAAGCTAGGTAACTATATTTTAAAAAAGTGTTATTTTAAAAGTTGATTAACTGCATCAATAACCCGTGCATTAATTTCAAACTGGTATTTTTCACCTCTAGCTAAAGGCACAACAATATCAAACCCAGTTACCCGTTGTGGGGCTTTTTTGAGATAAGTGAATAGTTCTTCAGTTACTGAAGTGATAATCTCTGCACTTGTAGTGAAACTTTTCACCGCTTCAGTCACTACAAGTAGTCTTCCTGTTTTCTTCACTGAGTTAAATACTGTTTGTTTATCTCAAGGGGAGATAGTACGCAAGTCAATTAACTCAATTCCCTTATCTTTCAATTCCCCGCTGTAAACTAAGTTAATTAAATCAAACATTGTAGGACCATAGCTAACTATTGTAAGTTCACTACCTTCACTAATCAAGTTGGCTTCACCAATAGGGACAGTGTAATAATCACTAGGAATCTCCTGACGAAAAGCACGATAAAGCTTCTTTGGTTCAAAAAAGATAACAGGATCAGGTGATTCAATAGCAGCTAGAAAAAGTCCTTTGGTATCATAAGGATTTGATGGCATCACTGTTTTAAGCCCAGCAATCTGTGCATAAATTGCTTCCAATGTTTCACTGTGGTGTTCCAATGCTTTAATCCCCCCACCCATTGGCATCCTCACTACTAGTGGAGCGGTATATACACCACGAGAACGGTTTCTAATCCTAGCAGCATGGACAAAGATTTGAAACATAGCTGGGAATGAAAAGCCTGAAAACTGGATCTCTACAATAGGTTTAAGACCACCTATAGCAGCCCCAACCCCAATACCGGCCATAGAGTTTTCTGCTATAGGACAATCCCATACCCTTTCACTCCCATACTTTTGTTGTAAACCTTTAGTTGCACGGAATACACCCCCTTCAAAACCAGCGTCCTGGCCATAGAGTACAACGTTTTGATCTCTTTCCAGTGCAAGATCCATTGCGTTGTTTAACGCTTCAATGTTATTTACTTGGATTTTTGACATAGTTATCTTTTTTAATATTAGTCTTTAAAGTATTTTTTTGCAATCTGTTTCTGTTCAACTAGTTCAGGGGTTAATTCTTGGTAGTTGTAATCAAACACCTCATCTACTGATACAGGGGTATCTAGTACCATCTTTTCATAAGCAGCTTGGATTTCCTGTTCAATTTTGCTGAACATCTCTTCTTCTTGAGCTTGGTTAAGAATTGATCTATCAAACAAGAAGTTTCGCAATCGCTTCACTGGATCACTCTTCATTCCCTCCTCCTCTTCTTGTTTGGTTCTGTAGATAGAAGGATCATCTGAAGTGGTGTGAGGACCTTGCCGGTAGCTGAAGAACTCAATTAGGACTGGTCCATTACCACCTCTAGCGTAATTAGCAGTATCTTGCATCGCTTCATAACTAGCAATTAGATCATTACCATCAACCCTTATCCTTGGGATCCCACATGCTATTGCTTTAACACTTAGATCACTAACAGCAGATTCAAGTTTAGTTCTTGTTGAGATAGCAAACTGATTGTTGTTAATACAAAAAACAGTGTTTCACTTGTGGATGCTTGCAATGTTCATCGCTTCATAAAATTCCCCTTCAGCTGTACCTCCATCACCAATCATAGTAACAGCAACATTAGGTTGCTTTTTATAGTGCAACATGTAACCTAATCCAGCAGCATGGGAATACTGAGCACCAATGGTGATGTTAATAGGTAAAGTTTTGTACTTAGCATCTATCTGACTACCTTTTTCATTACCATTTCAGTAGAGTAAAAGTTGTTCTGGTTTTACCCCACGATACAACATTAAAGCCCCACTACGAAACGTAGGACAAACCCAATCATTTTCATTTAAACCTAATCCCATTCCAACCTGTAAAGCTTCCTCTCCCAAATTAGGAGCGAAGTTTAACATCTTACCAGCACGCTGTCAAACTAACATCTTTTTGTCCATCATTCTACTCAAGTTCATTAAGTAATAAGCGTGTTTTAACTGTTCATCAGTTAGGGTAATTTTGTGGTTAGGATCAATTAATTTACCCTCATTATCATAAACCTGATAAAGGGTAGTTGGAACTTTATTTTTAATCAAGATTGCCATATATTTCTATGCTAATTTAAAACTAAGCTATTTTTTAGGAATGTAATAGCTAAAACCAAGTGCTTGTAAAACAGTTGCTAACACAAAGTTAAACGGTTTGTTGTAATGTGGAAGAAAATAAACATCCACTAAACCCAGTTCAGTTAGTAACATCTGCTTTTGGATTGCAAGTGCAATATAGAAAATAATCTCACTGTGATTGGTATTCCAAGAAAGGAGTTGTGCTCCTAAAATTCTTAGGGTTTTCTTGTCATATACAAGTTTAAAACGCACCTTGTCATAACTGCCCATAAACTCAGGACGATCATTGTCATCAACAACTGATATGCCAACATCAAAACCTAACTTCTTAGCACGCTGTTCAGTTAATCCACATGCTGCTAAATTTAAACCAAAGATATGGAGTGCATTGGTGCCAACGATAGATTGGAGTTTAACTTGGTTACTACCAATGATATGCATCGCAGCTACTAATCCACTCTTTACTGCATTGGTAGCAAGATCGATGTTTTCATACTGTTCACTAGCAGCATTGTAAATAGCAGCACAACCCCCAATGACATAAACATCCTTATGATTTAGTGCTTGGAGAAATTCATTAACTTTAATTGAACCGTTGTGAATAAACTCAAAGTTTTGATCTTTAGGAACAAACTTAGTGCTAGGTCTAAACCCAATTGATTGGATCACAAGGTCTGCATTTACTATTCCCTTATCAGTTTCAACTCCTTTGACAACGTTGTTTGTACTATCAACAACAAAGCCCTTAACACTGCAACCCATCATTAGTTTTAACCCATCTTTTTGCATCACTTTTTTAAGTTCATCAGTAAACTCATGATCAAAGTTATTACCAGCAGGCTTATCAAGTAAGTCAATTACTGTTACTTGCTTTTTGCATAACCAAGCTGCTTCAGCAAGTTCCAAGCCAATGTAACCAGAACCAACAATAGCAACTGATTTAATGGTTTTATCTTTACGAAAACTATCGATTAAGGTAAGTGCATGTTGGTATAACTTACAGCTAATTAAGTTCTTAACATTACCACAATATTTGTCAGTGTAGTTAAACTCCAAAGGCTTGTGTGTCACCTTGTTTTCAACATTCATACATATAGGTCATGCTCCTGAAGCGATTACTAGTTGATCAAACTGATCAGTGAACTCTTTATTTGATGTTAAATCTCTAACTTTTACCTGTTTTTTGATTAGATCAATGTTAATAACATCATGACTCATAAAGATGTTAGCGCCCATCTGTTTCAACTCCTTAGGGTTGGAATAGAAAAGATCATCAGTGTTTTTAACAACACCACTAACAGCAAGTGCAATTCCACACCCCAGAAACGAGATGTTTGTGTTTCTATCATAAGCGTTAACCTTAAAGTCCTTACTTTTTGAAAGTAAAGTTCTAATAAAACTAGTACCAGCGTGATTTATTCCAATCACAATCACTTTTTTCATATATCTTAGCTGCAGTAATTTTTTAAGCTATTTAAGGATAATTTGTGCAGTAGTGATTCGATCTTTTAAAATTAAGCTTTTGCTAGTAATGTAATAAAATCTTGCTAACCACATTTGGTTGTAACTTAGCTTTACCATTGAGTTTTTTCAGCTCAATTAAAAAACAATATCCCACAGTTTCACCATTTAACTGTTTAAGTAATTGGTCAATAGCAGCAACTGTTCCAGCAGTGGCAAGTACATCATCAACAATAACACACCTTTTAGCATTATTAGCTTGGATTAATGAAGTGGTTGACATCTCCAATACAGCATGTTTTCTGTACTCCAAATCATAGCTAGCACTAATTAATTGCCCTGGGAGTTTATTGGCTTTTCTAACCAATACTAACGGGAGTTGGGTTTTAGAAGCTAATGCTCCCCCAAAGATAAAACCCCTCGCTTCAGGACATACTATCGCTTCTGCATTAATAGCTTTAATAAACTGTGCCATTTGGGTTAGCACAAAATTAAATAGTTGGGGATTGGAAAATACTGGGGTAATGTCATAAAACGATGTACCTTGGTTGGGAAAATTTTCAAAGCGCTTGATGGCTTGATCAAGCAACTTAAAGTTTTGATCCATAAATATCTTTTTTTTAAAAACTGTTAATTCCTGCAATTAACTGCTCTTCAATCTGATCAATCTCTTTAAAAGGATGTTTTTTGTGTTTTAAGCTCAAACGGGAATGAACATAGTTGAAAGGACTGAGTCAACCATTGATTAAACCAAAGCAAAACATTGGTAAAAAAGCAGTTGCAACATCAAATAAACCAACAATAGCAATTAGATAAAAGATCACAAGGTTAAAGTTGCCATCAATCCCACCAAAGTTAAAACCACTGTAGATAATAAAAATGAAAACTAAGCTAGAACTTTCAATCAGATCTATCTTCCACTGCGAGTTGAAGTTATCGTTAAATAAGTTTTTGATAAATACCTTGTCCTTTAAGCTCCTTTTAAACCAGTAAAAGTAAGTCCAAGTTGTTGTTTGTGATACCTTAGCTGCAAAAAAGAGCCAAAACAGATAAACAAAGGTAATTAACTGGATTGTTGATTGGTCTATACTAAACAGATAGTTAGTTAGATACATCACTGCAAACAAACCACTACATAACAAGATAAAGCTAACCAAATAACTTAAACAATAAGCAACACCATATCTAATCAAACAATAAGCTAAGTACAGTAAACTAACTATTCCTATCCCAGCAATAATCCCGTTACTTGATTCAACATCTGCTGCTAAAAATTCTCCTAACAGGTTAGGTTGAACAAGATATAAAACTAGCATTACAACTCCTATAGCTAACAAGATTAGCCATACAAACAAAAACTTCCAGTTGTTTTTCAAAAAGAAATCATGTTGGTTTTTGGTTATAAAAAAGTCTGTGTTCAAGTCACTAGTTGAACTTAAAAGTGAATAGTTACTAGAAGTTTTAGTTAGTGCTTTTGCATCCTTCTGATAGAGAAAAAGCTTTCAGTTTGCACCTGATTCTGAACTGACAAACAGTGAGATTAAAACGATGCTGATTCCATAACTAAAAAAGTAAGAAGTAATGGCTGATATTGCCATCAAATTAGCAAGTTGTTGGACCTGATAATTAGAAAGATAGATAACAACTAAAGCACTAATTAACCAGGTAATGTGAAACTCAAGATTAGCAAAAAAGCTTCTTTTTAAACACAGTTTTCACGATTCGATAATAGAAGCATTATTTCTAATATTTCTTAAGAATAACTCTCCTAAACTCAAGAGATTAATTAGGTTGATGGCAATAACAAAGAAGATGCCCACAAAAGAAAAGACATCAACAACTCCACCAACTGCACTAAAGATAACCAGTGATGACACCACACTTAACCCTAAAGCTAATGCTTTGTATAACCCTAAGAGCTTGTATCGTAGTGTTAATAGAACAGCAGCAATAAGAACGATGATACCAAACGCAATAAAACTAGAAGCAAAATTAGAAACATCTGTGATGGTATTTGCAAGTGGAGCGTTAACCAGACTTGTTTTTGCAGTTGCAATGGTAGCTGCTGTGGGAAATGGATTTGTTAAAAGCTCTTTAACAGTAGCTGCTGTTACAAAGTTTGGTGGGGTGTATGTACCATCACTACTCAAGAAATTACCAACTTGAAACTTAATATTACCACCATATTCTATCCGTTGGTTGTTAGTGTCTAAAAAGCTTTGTAGAGTAGGATTAATCTTAACAGGATCACCGGTTTTGCTAACCGGATCTTCAGTTCTAATTTCACTGATAATATAGTTATGGAATAACCCTGAAAAACCACTGCTATCTGCACTCCTTAACTTTCTTGTATCAGCACTATTTGAAACTGTAATTACATCTGAACTATTGTTGTTATTGTTATTATTCTGGTTATTATTGTCATTACTGTTGCTAGCAACCGCTGAAAAGTGGTTAGGAGCAGCGTAGATATAATAAAGGTTTTTGAGAGTAATGTCACTTGCTTTTTTAATAGGAGATGCCCCATTAGCATCAGTGTTTAAAAACAGATTATCACCACTAGCAAAGCTACCCTTTGTATGTAAAAATTCCCAGAGACTCTTCTCTCTTTCATTAAAGGTGAGATAATCACTACTACCCTCAACTACATTAAAGATATTACGGACATAGTTTAATGCGCCTTGTTTATCTTTTCACAATACTAAAGTTTTATTACCACTACTTGAAGTTCCATTCGCATTTTGCTTAAAAAATTTACCATTGCCCTCAACACTAGCTTGCCCATTACCAGTATTATAATCAGAACTTGATAAGTTTTTAGAGTTGATAAAATCAAGAAAATCGGTGTGTAAACTTTCTTTGGTAACTACATTTTTATTTAACTTGTATTCAAAGGTTAAAGCACTGTTTACAGAAGTGTTTTGGGGAACAACTGCTCTAATGGAAGCATTAATAAAATTATTATCCAATGAATCAAATAAAACCACTCCATCAGTTGTTTCAAGTGAAAGTTGGTAGTTGTGTTCAACTGAACTAATTGCATTTCTTCTAACCTGAGCATCAACTTGTCTTTTTCTTTGGCGGGTTTTGGCTTGCTCACGGTTTTGTTCAAAGGTGAGAGTAACAGAAGGTAATCCCCCATTAACCAATCAGGACTTGTCAATGTTGTCAAGTTCACTAGCAGTTTTATCATTAGTGTTTTTAGTAATGTTTACAGAAGAAAAGCCCTGAATGAATAAACTGTTAGCATAACTTTTTTCAACACCATCTAAAAAGCTATCAATGTTAGTGATGTTTTCAATCCCGTTGGTTGGTTTTGTTTGCTGAGGATCTAATGAGGTATTGTTAGTTGACTTATGGTTTAAAAAGTAAACAGTTGTAGTGGTAGAACCGTTGAAAACAGCACCTAACCTGCTGTCATTTAACAGTTTGTAACTCCCAAAAATAACACCAAACAAACCCAGACAAACAAGTCCAAGAATAGTTCCTATTTTTAGGATTCAGTCCAAAGAAAAGCGCTTTTTGAACCTCACTAACTATTTTTAATAACTTAAATTTTATATAAATATTAAGTAATGGCAACCATTCAGGAAATTGAGTGTGATTTTTTAGCTAAAATAGCACAAAAATTTACTAATGCAGAGATTGAATTAATTAACAAAGCATTCTATCACGCTAAAACTTGGCATGAAAACCAGAAACGGCTTAGCGGTGAACCTTTTTTTATCCATCCTTTAAGAACGGCATTATCACTAGTTGAATGGAACATGGATCCTATCACTATTTGTGCTGGTTTGTTACATGACATCATTGAAGATACAGACCAAACCGAAGCTAATATAGCAATGATTTTTAACAAAGAAATTGCTGAGCTTGTCACTAAGGTTACAAAGATTACCAATGAATCTAAAAAGCAACGTCATCTCAAAAATAAAAAGGAGAATCTTAACTTAAAAAGCTTTGTTAACATTGCAATCAATTCTCAACAAGAGATAAATGTAATGGTACTAAAACTAGCAGATCGACTTGATAACATCGCTTCCATTGAGTTTCTCCCCATTGAAAAGCAAAAGGTAATTGCAAAAGAAACTTTAGAACTTTATGCAAAGATTGCTGGGAGGATTGGGATGTATCCTGTTAAAACAAAATTAGCAGATCTTTCATTTAAGGTGTTGGATTTAAAAAACTATGATAACACCCTGTCAAAGATTAACAAGCAAAAGGTCTTTTATGACAATGAGTGGGATAACTTCAAACAACAATTAAAAAAAATCTTAGCGCAAAATCAGATAGAATACCAACTTGAAAGTCGGATTAAAGGCATTTACTCTACATATAAAAAACTAACTGTTCATGAACAGAACATCAGTAAGATCCATGATCTTTTTGCTATCCGCTTAATTACTAAATCAGAACTTGATTGTTATCACATCCTTGGTTTAATTCACCTTAATTTTTTAATTGACAGTAAATACTTCAAAGACTATATTGCCTCACCTAAACAAAACCTTTACCAATCAATTCATACCACTGTTCGTTTAAAAGGGTTAAATGTTGAGATCCAAATTAGAACCCAACAGATGGACAATGTTAGTAAGTTTGGCTTAGCTAGTCACTGGATCTACAAAGAACAGAAAGAGGGATTGTTAGCACCTGCTTTGCAACTTAATTACCTAGTGACAAAACAAAAACACTCACATGATTTTCTAAAAAGGATTTTTGGGACTGATATTATCAAGATTAATGTTAGTGCTAGTCATGAACCTAATGTAATTAAGCAAATTAATGTTGATAGCAACAATAAACTCCTTGATATTGCTTTTGAAAACTATCCCAAGCAATTTGCTAAATTAACCAAAATTGAAATTGATGGGGTTGAGATCAATTCTTTTGATACTAGTGTTGAAAATGAGATGCTGATTGAATTTTACTTTGGCAAGAATAACAATTTGAAATCAAAGTGAATTAGGTATATGAATAACCCTATATACCGTGAAAAGGTAAAAAAGAGCTTGGCTAAACTAGCTAAATCTGGTAGATACAGTGAGTTAGCTTCTTATGAAAAAGAACTGGGTGAAAAACAGTTAAAACTTGCTAGTGAAACTGAAATCCAAAAACGCTTAAACACCCTAAGAATTAAAAAAATGAGTGATTACTTAGCGTTAATTGAGTGTACTAACTTTACTAATGATGAACATTTGTTGTTTCTAGCTAAAAACAACGACAAGTGAAATAAACTAACAAAACCACTTAAGTTTGCTTTTTCAAAAGTAGTTTTTCACAACTCTTACTTTGAACAAATTGAAGGTATTTTTATCACCAAAATAGTGATTGAACCATGTTGTAGTAAGATCCCTGATATGCCTGAACAAGTAACTGGTATCTTAACTAAAAACATTTTAAGTGTTCACCGTTATGGTTGTAAGAATTTACAAAATAAAAAGCAGTTAAAAATTATCCCGTTATATTGAAATATCCAGCAGTTAAAACTAAAACCACGTAAGTTTCGCAGTTACATTAACATTAACGGAGTGTGGAGTGAAAAAACCATTAATAAAATCTGTCAAACAATTATTAATGGTGATGGTTATATTGAAAAAATAATTCCCAAGATCAACAAACAAAAAGATGAATTTGATTTAAACATCACCCTTTTTGTTAATAACTACCAACAACTTCTCACCTTAATGGACCAAATTACCACTAAGAATATCAGCTTTAGTTGAAAATACCTTTAGTTACCAAACACCGCATCATACACTTCTTCATCTCTTAAAAGTAAAACTAAGTGCTTTTTTAGTAGCCGTTGTGGATTGAAACGTGATACTCTCACTCCATAAACTTTCTTTGTAAATAAAATTAATAAAAAACCAGAAACAAAAAATGCCCCAATAATTGAGATTCCTGTATAGAAAAGGATTTGGGAAACTTCAGTTAAATACCGCTTAAAATCATCATTTTGGTTTGGATTTGGGAACTTTTCTAGCATTTGATCAACAAAGCTAGTTATGTTATTTAATGTGTGTTTAATTTGGGTAAAAGCAGCATTACTATCAAAGTTATTAATTTTTTTCAATGAACCTTCAAGTGCATTTAAAGAACTATCTAACCCTGATGCAAATGAATTGTTTTGCGTTAATTTTCGCCCTTCGTTTAAAGATTTTTCAACTTCTGAAAGTGTTTTAACTAGGTTATTTTGGAAGATATTTTGGGAGATCTTTTTAATTTCAGTGTTATATTCACTAATCTTGTTATATCCCTCTTTATAGTAGTTGTTAATAGGTTCACGGTAAGTAAAAGCAAAATAACCAGCAGTAGCAATTCCACCTAACAATAAAATTCCTACCAAAATAATAAAAAAAACTGCAAGTTTTTTTAATAGTCTAATCATGGTCTAATGCTGTTATCACTTCCTCTTCCAAATCAGGATAACGATCTAAGATATCATTAACATGATCAGCTAACTGTTCAGTACTAGTAAATCTTGAAAACCTGATTAATCCCCTTTTTTTAGAAGTTAAAAAGGAAAAGACAATGGTTGTAATCAAAATTGCTAGCATCCCACCTGATACAGCAACAATGGTTATCATTGTGACATCATAATAATTATTGAACTGTTCAGAAGAAGGGATGTTGTTTTTGATCTTTTCATAGCTACTAACAATGCTTTGTGCTTGTGTAGAAAACTCACTAACTGCACTAATTACACCATCAGTGGTTGCTTGTTTTTTTACAGATGGAGATGAACTAACAGTACCATTTTTATCAAGATTACTTTTAAAGTTTTCTAATTGTGTTTTACTGTTTTCCAAGTCTTTTTTGGTTGCATCAAGTTCTCTAATCTTTTCTTGACTAATATTACCTAGGTTGTTCTGACTCTCTAAGTTCTTTTTGTATTCATCTACTTTTTGTAAAGATTGCTCTAAGTCTTTTTTAACTTGATCTAAATTGTTAGTAATGGAACTTTTGCTAAATTGGTTAGCACTATTTGCTAAAGCATTAGCAAAGGCTTTAGCAGAATTGAAAGAACTATCAATGGAATTACGAGCTTGAACATAAAGGTTAGCTGCTGTACTACGAAAATACAAAGCAGTAATACCAAACCCTAACAGAAATACAAAAACCAACATTCCAAAGAAAAGCAAGATGGTTTTCGAAAACCGATTAATAAAAATAACCATTTAAATACTTGTACTTTTAACCAAACTAATGGTTATAAAAGTGTTTTGAAAACAAAACTACTAGAGTTTAAGTTTAACACCCAATGCGTTGGTTTTAAGTTCTTTTGCTAGTGATTTAACTTCATTTGTATCACTGGAAACACCAGTAATATCAACTGTTTCAAACTTTTCTTCACCAATTACAGTAACCAACCCTTCAACTGCTTTAGTCATTCTTTCAAGACTACTATCTGTATCATTTTCTAGTTTTTGGAACTGTACACTGTATTTACCACTAGCAGTAATTCTTTTGATGTTTTCATCAAATGTTACTCCGTTTTGTTCAGCACGCTTTAAAATTTCATTTTGAAACTCGCTGTCTCTGCTTGCACGTTCATTTTCATAGATGCTAGCACCAGGGTAATAGTTTTCATTAACCCTGTAATAGGTGTTATCTCCTTCTCTGTAAGCTTCTTCTAAGTGGAGGTTAAGTTCTTTTAAAGAACGGTACACCAAATCATCATCACTATCCTTGTTGGTATTAACATTCTTAACTAAGTACTTGTCAATGTATCCCCCAGCAAAATAACCTTGGAACTGTCTTTCAAACCTTCTGTAGTTGTAAATATCACCAACTGCTTGTTTGAGCTTATTAGCATCAATGGCTGAATTATCACTGTTTTGGAGTGTAACTTGGGTTAAATCAATGTGGGTAAATGGTTTTGAGGCAAACAGATCATAGATAACATTAGTTTTACTTCCCAATACTAATGGGTTAAAACCAAAACTATTTGCTTTACTGGTGGAATAAAATTCCAACTCAACTACGTTTGCAAAGATTTGTTTAGCTACATTAACACCAGTGTAATCACCATAAAGTGAAACTTTTTTAACTGATTCTGGTAATTGCAATTCATTAAGTTGTGAACCATTACCACTTAAACCTCTAATAATTAGGTAAGTAGGTTTTTCTGAATCTATTGTTGTTTTTAAAGCACTGATATCTTTAGCATCAATTGCGAGTGTTTTAACATCTTCAAAGAAGTGGTTTGGGTATGAGTCATAAAGTGGTGAACCTTTAGCAGCAAAATAAGTTCACTTATCATAAGTAAGGTTATTACTAGTTGAAACAGTTGTTGCTTTTGTGTTACTCCATCCGCTGAAACTAGGAACTTGTCTAAACACATCATCATAACCACGGTTGTTTGCCATGAGAGTACCGTTAGTTGTTTGCAAGATTGGCATCAACACGCGGTTATTGAAATATTCAGTTGATTGGTAATAGCTAAGCAGTGAACCATCACTAGCAACATGATCATAATAACCAGCTTTAAAACTAGGAGTAATAAAGTTATCACCTGTTTTAATTAAGCCACTAATTTCAGTTTTAGGAACAGGACGGTCTAAACCCTTTGGACTGTTAGTGATCGCTTCTGATAACTCATTAGCAAAGTTTCTAAATTTCTCACGGAAGTTAGCACCACCAGATAAATCAATCTCACTCTGATAGCTACCATCGTTTAAAGAAAGTGATTTCAACTTTACTTCTGCACTAGAAGATTGTGTTGAGAACAACTTAAAACCGAGGTTACCACTCCTACCTGATGCAACCAAAGCATAACCACTTTGATTAACTAGGTTAGTGATCATAACTGCTGAGAATGTGGTTAACGCTCCAGCACCTAAAACACCAATAGTCCAAAATAATTTACGCTTAAATTTCACACTATTCTTATGTTTTTTAAATTGCATTATTTTTTGTATTATTAGAAACTTTTAATTTGTAAACAGTAGTCTACTTATCAATTTTAAATTAAATCCACTAGTAAACTTCAATAACCCTTTCTCTGAAAGAGCTTGGTAATTTATTAAACAAATAAAAAACACCCTTAAATTGCTTTAAGGATGTAATGAGTTTAAATCTTTAATTTTTGGAGCGAGTAATCGGAATCGAACCGACACGATCAGCTTGGAAGGCTGATGTTCTACCATTAAACTATACTCGCACAAATTTGGTGCCCAAAGCCGGACTTGAACCGGCACACCATTACTGGCGTGGGATCTTAAATCCCATGCGTCTACCATTCCGCCACTTGGGCTGGTGACCCACCCGAGGATCGAACTCGGGACCCTTTGATTAAAAGTCAAATGCTCTACCGCTGAGCTAGTGAGTCTTTTTGATAGGAATTTAACCCTATCTTTAGAAATTTAAGCTTTAACTGGCTGGGATGGATGGGATCGAACCATCGCATGATAGAGTCAAAGTCTATTGCCTTACCGCTTGGCTACATCCCAAAAATGGTGGACAGGGAGAGATTCGAACTCCCGAAACCATAAAGGTGTCTGATCTACAGTCAGAAGCGTTTAGCCACTTCGCTACCTGTCCATTACTACCAGCAAGATATTTTACAAATTATCTGTTGCTGATCTTCAGGATTTTAACACGATAGGGAACTTCAATACCAACAACATCACATTCATCACCAATTAAACGCCCATAGATTGCAAGGGCAAGGGGTGATTCATTGGAAATTTTGTGTTCTTCAGGATTTGCTTCAAGTGTACCTACAATTGTGTATTTTTCGTTGGATTTAGAACTGTAATCATAGATCTCAACAGTGCTCCCTAAGCTAACTTTTGTTACTTTGGTTTTTGCTTGGTGGTCACTAATTAACTTAGCGTTGGCTAATATATCTTGAATTTCAGCAATCCTAGTTTCAATCTCACCTTGCTGTGCTTTAGCTGCATCATAATCAGCATTTTCACTTAAATCACCCTGATCACGTGCTTCTTGTAAGAGTCTGATAATCTCAGGACGTTTAACTTGGATTAGGTTTTCAAGTTCTTTTTCCAGTTGCTTAAATCCTTCTTGAGTTAGGTAATTTTTATTTAGTTCCATGACAAAATTAAAAGCAGCAAAAACACTTTTATTATTTAATTATTTCCCCTTTTTTTGCCAATTTTTGCTAAAAAAATAGAAAATCAGTATTGCAGTAATATAGATATTAAATAATAAATTTCTATCTAACTTCGAAATAATAAACTCAAGATCAACAGAAGATTGTGAGTTTTAATACAGATGAGAGAAAACAATAGTAATGCTCAAAACAAAAAGAAGATCCGCTTTTTAGCTTTGGGCATCATTATTCTAATCGTACTAATTGCTTTAATTACTATGCTTTTTATCACTGGAGTAATTAGTCGCAACCGTTAAGATTACTTTTTGCTAGATGACAAAAAAAACACAAGATCTCACTAGTTGGTATGACCAACTGCTAGTTAAAGCAAAGTTAATTTGTCATGGTGAAGTTAAAGGTACAGTTTGTTTTTTAAATAACAGTTGAGGCTTATGGATGGAAATCCAACAGCTTTACAATGATGCAATTGCAAATAAAAATCAATTGTCTGCAATTGCTCTAACTAAATTCCAACCAACTACTAGTTTTTGTTATCAAGTATTCCAAGTACAACTCCCTACCCTTTCTTTTTACAGTGAATATCAAAAGGAAAAAACCCATATCAAAGGTTTTAATCCTGAGCTTTTTTTAATTAATCAAGTTGGTCAAAAACAACTCAATGATCCTTTGGTTTTACGACCTACTAGTGAGATTGCTTTTTGCAACTTATGGAAAAAACAAGAGTTATCTTACCATGATCTACCTTTAATTTATAACCAGTGAACTCAGGTTTTTCGTGCAGAAAAAAACACCAGACCTTTTTTGAGAAACAGTGAGTTTTACTGACAAGAAACTCATGGGCTTTTTGTGGATCAGAGCCAATCTGAACAAGCTGCTATTAGCTTTTGAAATTTATATCAGGATTTAATTATTAACAAACTTTGTATCCCTGCTTTTGTTGGTTTGAAAAGTGAAAGTGAAAAATTTGCAGGTGCTAAAAACACATGGACAATAGAAGCAATTATGCCTGATGGACAAAGTTTGCAATGTGCCACTAGCCATGATTTAGGTGACACTTTTACAAAGAGTTTTACTATCAGCTATCAGAGTAAAACTAACCAAAAAATGACTCCAAGTAGTTTTAGTTGTGGGATGTCAACTAGGATCTTAGGAGCAATTTTTTTAACCCACAGCGATGATTATGGTTTGGTTTTACCTTGGTATCTAGCAAGTAAACAAGTCAAGTTATACCTGTTTGATAAAAACAATAACCCTAAAACAAGAGCTTTAGCTTTTTTAGTGAAGGATTTTTTAGAAAAACTCAAAATTCGCTTTAGTTTTATAGAAATTAACAATCAACTAGGTAAACAACTTTTAAAAGGAGAAATAGAAGGTATTCCATTACAGATGATTGTTGATAATGAAAAAACTATTAACATCTTCAACCGCTTAACACGTTTAAAAACCAGCTTAACATTTGCAAATCTCCAAACTGAATTTGTTAATTTAGTTAACAACTACCATACAGAGATGTATAGAAAAGCAAATGATTTAGTTGAACAAAAACTAGCAAGAGTACAAACTTTAAAGGAAATTGAACAAGCATTCAAAAATAAAAAGGCTGTTTTATGTACCGTGAAGTTAACTGGTGAACTTGAACAACACTTAAAGACAAAATACCAAGTTAGTGTTAGGTGTGTTTTTAAAAAGTCAGATGTAACACAAAACTGTCCTTTTACAAATCAACCTTGTTTTGATTCAGTTTTAATTGCACGTGCTTACTAACAAAGTACTGTCAATCACTACCCAATAAAACCCTTGATTATTTGGTTTTATTTAATTTATTTCTAAATATTTTTTACCAGTAATTTTTGTTTAAAAACAAACTCGTTTTGTAAAAGAAAAAAGTTAGAGTTGTTGTATTTCAATAACGATTATTTAGCCTCTAAAATCGATTAGACGATCTTCTGATCGTTTAAGATTTATTCAAATTCAACATGACTTTATGACTTTTACAAACAAAGAAAAAAATTGCAAACAAAGCAAATCATTAGCAGCGTTGATGACAAAATTTAAGCGCTCGCAATTAATCTTGAAACACCAAGCTAATAACATAGCACTGGAGTTGTGAAATGAAAATGATATTAACCTATCAAAACAGCTTATTGAGTTAATAGAAGATACTTTTTCAATGCTTAAAAAAGAAACTGTTGATTTTATCTATGACATCTATATTTATGGCAAAAAGCCGTGTGATATTGGTTATTCTAATTCCACCTATTACAAAAAACTAAACAAAGCAGCTAATAGTTTTTTTGACCATTTTGTTTGAGATTCGTCAATATTAGACAAAAGGATCATAACTAATGGCAGCAATTCTCAACGTACAACGAATTCAAAATAACCAAGTCACTGAGTACACAATGAGTCCAGTGCGTAATTTTGCAAACACAAAAGATGTATATTTTGATGCGCAATTAACAAATATAGAAAGCAAAATTGATAGTAGTAGGGCTCAAATTCACCTTACTATTGCACTAAAATACAACACTAATCTCCCTGATAATATCTTTCAAGCCCACTTCAGTTTAGGCAATTGACAAAGTGATAAGATTCAACTTCAAAAAGCTCCTGATAAAAAACACGATAGTTTAAACAGCATTAAATATTTTTATGCATTTCTAGATGTACCCCGTTCAGCACTAGCAAAAAAAGAAATTAATAGGTTTAGTAATGTGGTTGCTAGAGTATTGAGAATAAGTTTTCGCTTGCAAGATCAATCTGAAAAAGGGAATTGAAGTGACTATCATTTGTTTGATACTGTAGCTAGTGAATTGTATGCAACAGTAATTAAAGAAACAATCAACTTTGGCAACATGATTAAGATTAATGCACTTGATGGTAGTAAACAGCTAACTAGTAGCCAAGGAAGTTTTAAATACAGTTGAACAATGTATGACTATAGGAATTTAGAACAACTTGATGAGGTGAGAAACTTGATCAATATCAGTTTTGACAAACCAGTTCAAATAGTAAATGTTGATGTCAAAATTCACTATGTTCCAACTAAAGGTAGGTTACAAGAGATAAAACAACAAGGTGAGTTTGAAAATAACCTTGATGTTAATGAAAAGCTTAAACTCAATTTAATAGGCAATTGAAATTTTGACAAAAATAACAAGAAGCTAATCAGTGATATTTCAGGTACTGGAATCTTTTTACCTCAGGGTGGTTATGGGAGTTATGAAATAATGATTGGCGTTACAGTAGGTAATGATTTCTACACAATTATAGCCAACAACCAGTTTAAGTACGAAACACCTTTAGATGATCTTGAACAAAATGACTTTTTTGAGGTAAATTATTTACCTGTTTACAGTACATACAACTTTAGTGATTTAACCCAGTAAGTATGATTTTTTCAATTAGTAAACGAAAGTTAATTTGCGGATTTTTGCTAGTTATTCTAACAATAGGTGGGGTTTTGGGTGGTGTTTATTTAGTTACTAAAAACAACAAGGATAACTACCAAAATGAAAGTAATTTCAACAATCAAGAACAAATTAGTAAAATCCCTAATTTCAAAGCTATTGGTCCTGAAACACAACGTATCTTAAGAGAAAGAAACTATCCTTTAGATGATAGTGGTTATTATGTTTATAAGTATGGTGAAATTAATAGGTATCTAAGAAATGAAAGTGAACTAGATGAATTGATTAACTATAGAGTGATGGTACCTTCACTTAAATCACATCACAAAAGAGTTAATTTTGATAAGGCATTTCTTGAAAGTAAATTAAGAAAATGGATTATCAAAGCAATTAAGCAACATAACTATTTCCAACACTTTGAAAATGAACCAAATTTAAGAGTTCAATATAACATGAATATTCCAGCACAAAAAATTGATGTTAATGCTGTTTGATCTTATAAAAAAGATAATGATGCTGCTACTGGTAAGCCAATCCGATATTGAGATCAATTTGAGCTTAAACTTAAATAATAAATCTTTATAATTTTTACTAGATTTACTAATGCAAACGCATGAAATTCTTTTAAAAATTAAAGAAATTGCTAAATCAAAAAACTTTAATCTTAATTTAGATGAAAAGACAATAAATCAACCACTTCGTGAGTTGAAAATCGATTCACTTGATATGTTTAGTATTGTTGTTAGTCTAGAAAATGAATTTGGGATTAGTTTTGATGATGAAAAGTTAATGAATCTAAAAAATCTTGCTGACTTGGTTTTAGAAGTTAAAAACCTTTTAGCAAAAAAGGGGTATAGTTCAAAGGTAGAACATCTGTCTTCAAAATAGAGTGTTGTGGGTTCGAGTCCTGCTACCCCTGCCATAAAATATAAAAGCTTAGTAAGTCTCTACTAAGCTTTTTTCAATCCCCCCATTCCCCCAACCTCTATTTTGTGTTACTAGTGCCTAAGGTGGTAGTGGAGTATCACAAGCTCAGTAAGGATGTAGTCAAAGAGAGTTTGGAAGTGGAAGCAACTTCCAACTTTGATCCAACTAAAAGGTTGCAAAAAGAGAGTCCAAGAAAGCGATTCAAGTAAAACAGGGGAGAAACTCACCGAAACAACTTCATCGATGAGTAGTGGTGGGGCTACATCCACTCGCAAGGCCCTCAAGGTTGAGGTGGAAAGGGGGAGTCAAAGTGATTCACTTTTAAAAAACGACTTTGCTAAAAAGCCGTTGAAACATAAGAACAATGGGGATGTGAAGTTGGATGCGAGTGGGGAGTTTGCCAAAGCCTGAAAACCGGTGTTGAAAACAGATGAGATAGCAAGAGAGAAGGGGATGGGGGCGACTTAGACTTTCTCCCCTGAATCGGCAATGGTAAACCCTTCTCCAACTCCCACTCTCCTTCCCCTTCTACTTCTTCTTCTACCCCACTCCCCACTTTTTCTAACATCAATGTTGGGGTTAAATCTAATGATCACTCAACATTTAAATAAAGAAAACACCCGGTGGGTGTTTACCCCTAACTCTTCACCAGACATCTGAACGGGAGCAGGTTATAGAAAAGCTAATAACAACAATAACGGCATCTCCTTGACAAGTGTGTTGCCTAGTAGAATAGTAGCAACAGTTTGATCCCTCCTCCATGGAAAACCAAGTCACATCATCATCAGGTGGCTCCCCGCCAAAAAAACAACCACCTATTCCTTTTTACCCAATTCCATCAGTCCCACCAGTGACTGGATCAATGCATTGACTTTCACTAATAAGAATAACCCGCAACGAAATCAGTTGTTGCTCAGAAGCTTACTAGGAACGATCCCGGTATTGATCAATAAGAGTGGAACGGGAGATGAGTTTAACCATACGAGTGACCAGAAGTGGGATAAAACGAATGAAAAAGAAGGGAACCTCCCTGGGTTTGGGGAGGTGAATGGTGGTTTTTATCAACTAAATAAAAAACTTATTAGCTTATTTTTATTAGGTTTTTAATTATTTAATAATTAAAAAAGTTTTGAATTTTTCTTAGTTTTTATTTGTTTAATAGTTAAAAAACACTAGGCTTTACCTTTATTTAATTAATAAAACCTCGACCCCTATTACCAAACCATCCATAACACCAACTTGTTTGTGTTGTTCAAGTCTAGGGATGTGAAAGTTAAGTATGAAGTCAAGTGGCTCAAATACCAACACAATTAGTTTTGATTCAACTAGTCAAGCTAACAAACCATCCTATGTGGTCGAGTTTACTAACTCCACCAACATTGGCATCAAGTGAAGGATGGTGAAAAAGTATAAGTTAGATGTACCAAGTGTTTCAACAACCATGAACCAAGTGTTGCAAAACCTCATCCTAGAACAACCTTTGACTAAATATACCTTAAATAGTAGTTTGGCCAAACAAAAGGGTAAGACACAAGCAGTGGTGCATCTTGGTAATGGGCAAGCAAATAATTGACAATCGATGCGCAACCAACACAACCTAAACAACAATCCCTCTCCCAATGCATCAACCGGATTTAAATTAACTAAAGGCAATGCTTATAGAAAACTAAGTGAATCCTGACCAATTTACCAACCAATTGATGGGACCAAGCAGGGCAAAGGGAAGGATAGTAATGGGTGGAGTTCAACAGAAGAAACAATGGCAGCAGAGGATGCGCCCAGTGTTTCAACAAGTGGGGGTGGTTCTTCTGGAAAATTTAAAAAATACCTCAACACCAAACAAGCGTTAGAAAGCATCGGTATCTTGTTTGATGGGGATGGAATGAGGAATGTTATCACCCAGCTCTATTATGCTTCAACTAGTAAGCTAGCAGTCACTAACAACCACATTGTCGTGATGGGTAACAGCTTTCTACCCAGCATGTGGTACTGGGTGGTGGATAGGAGTGCAACAACTGATTCATCATCAAAACCCACCTGGTTTGCTAACACCAATTTAGATTGGGGGGAAGATAAACAAAAACAATTTGTAGAGAATCAGTTGGGGTATAAGGAAACTACCAGTACCAATTCCCACAACTTCCATTCCAAATCTTTCACCCAACCTGCATATCTGATCAGTGGCATTGACAGTGTCAATGATCAGTTGATCTTCAGTGGTTTTAAAGCGGGCAGCGTGGGGTATGATAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTACCAAAGACCAAGCACTTGCTTGATCAACCACTGTTAGCTTAGATAGTAAAACGGGGTATAGGGATCTAGTGACCAACGACACGGGTTTAAATGGTCCAATCAATGGGAGTTTTTCAATCCAAGACACCTTCAGCTTTGTTGTTCCTTATTCATCGAATCATACAAATTCAAGTGGTTCATCAGGAACCATTAAAACGGCTTATCCGGTGAAAAACACAGAGAAAGCAAATGTAGCGATCAATTCTTTGATCAACGCTACGCCGTTGAATAGTTATGGGGATGAGGGGGTTGGGGTTAGAAATATTTTTATGAGTTTAAAACAAGGTAATGCAGATAACTAGCTAAATCAACAACTGGAGGTTTTGTACCCCCATTTCTTAAATCTAAATTATGTAAGTTTTGCTGTTTAAGTAAATCCATAAAATTTTTATCTTGAACTTTTATCTGCTCTTTTACGTGTAAAGTGAAAAGTAGCTGATTGTCTTTAAATTGAAATAAAGAACCTGGATAATCAGTATTTTGAACTAAATAGCTATCCAAATCACCATCAACGATAAATTTAACATCCATTTCACCTTTTCATGAAGATGGAATACCTATTCATGGACCAATAAATTTAGATCAAAAGTTTAATTCATAACCACCCTGGTATTTTATGTCAATTTCAAATTTAAATTTGTATGGATATTCTTGTCTAAAATCAATTCCACCTTCTTTTCATGAAATTGTTGGATTATTTTCCTTGTTTTCAACATCAACCTTATTTAAATTCAAGCTTATGTTAGTATGTGACCTCAATTTATCTCTTAATCAGTCCGACATATAAAGACCTTCAACTCCTTGTTTAGGAAACTCTATCCCCTTTTCACCAAAAGCTTTTTTAGCGTTTTGATTTTCATCTTTTTTGTTGTTTTTGTAATACTCCTTTTATTGATTTAGTAAAGCTGTCAATTAAAAATTTGAAGGTCTTATTTTTATCAGCAGCAAAAGAAGTTTTTAGAGCATCTATTAGTTGAGTCTTATCAGTAACATTTTTGCCTTTTGTAGATCAATAATTTTTAAATTCAGTTGCAGTTGAAAGCGACTTATCAAATTTTTCTTGTTCAGCTTTTAAGCGCTTAGCTTCCTCTTCTTTTCGTTTAAGTTCTTCTTCTTGTTTTCTTCTTTCCTCAGCTAAAATTCGTTGTTGTTCTTCATAATCAGCTTTAGCTTTTTCACGTTCTTTTTTAAATGGTTCAATTATTTCTTTTTCAAACTTTGTTTTAGCTAAATCTAGTTGTTCATCAATTTTTAAAACTGAATTAAAGATATTTGTATTGGAAAGAAAAAGTGAATCACTTACAGCAGTTTTAGCGATTGAAGTTAGATCATTTTGATTTTTATCAACAAGTTTATTAATTAGAGGTTTTCAAAACAATTGAGTATTTATATCGTTACCATTAAAACTATTTTGCAACTCTTGTAAATTCAAAAAATTAGAAGTTTGGGCTTTTAAATCAAATTGCAAGTTAACAGCGTTGTTACCACCTTCATAATCAACTTCAAAATCCATTTTGATTTCAAAGCTATAACGAATCTTATCTTTAATAACAATGTCATTTTTTTAACCAATTCAAGATTAAAGTCTTTTAATTGAATTTTCTTTAAGTTGTATTGATTAAATATTTCAACAATACTTTCAGTAATGTTTAAACCTTTTGGATGCAATTGTGGAAAGAAATTTCCATTAAGGAACTTAAAAAAATTCTGTGGAACTTTAGGCAATTTATCTTCATCATCATATTGGAAAATTTTGTTAGTTTCATTAATGCTTGATTGCAGCCTATTTAATAAATCAACTGTTTTTTTAATACCTGATTTAAAACCACTTAAAAGATGAAAAATATCTATAACATTAAAGTTCCTAGGATCTAAAACTTCATTCTTAAATTTCTCTCGAAATAAGTCAAAGTTTTTATCATAATCTTTCTCTTTAGCAATATAGTTTTCTTCTTGCTCTGTTATAGCTGTTTGCTGTTGGAGTAGAATACTATTTTTCTCTACTATTGTTGTACTTGATTTTCTTAGACTAGGTTGACTTAAATTTGCAGCTGAGCCATTATAGATAGAAGGGACAATAAGTACACCAGCAGTTAAAGTTGATGCAAAAGAGACAAAGAGAAAAGTTTTAATTTTAAAACGAGATTTTTTGATAGCTTTCAAAGTTAATAATTAATATTGAAAATAATTAGCTAAAACTAAAAAAGTTACAAACGAAATTGAATTTGTTTAAAACTAAAACAGACAGGGAAAATTTAACATTATATTCTAACATCTGAATAGCAAAAAATATAAGAATAGCTTTAATTCAAAAAAGTAATTCGGATCTTAAAAAATATTTTTTAATAAAGATTAATTGCCAGCAACTTGAATCTAAGTAATAAAGTGAATATTTATTTTTATTAAAAACTTATATTCAAATTTGTAGTCTATTTTTAACTAATATAAAAAAGTTGAATATTTAAAAATAATTAATTTTGATAATTAATAATCCAGGATTAATAAGTGCTTTTCAAAAAATTTACTTGGGTCATTCCAAGTTTGTTTTTAACAATTATTTCAACATCTTTACTTATTAGTTGTGCAACTAAAAGCGATAACACCTTAATATTCAATATTTCACTTGATCATAACGCTGATACATCAATAGAAAAATTCTTTACTGTTTTTTCAAAAAAACTTAGTGAAAAACTGAATAAAAAAATTAATGTTAACTTTAATATAGTTGATGATTCCTTTACAAAAATTAACAATATTCAAGCTAATAAGGCAGATTTTGCTTTTGTTAATTCGCAAGCTATTGCTTCAAATAATTGGTTTGGCTATACGCCATTAATACAAACTTTAACAACAGCTTTTAAAGAAGATTTGGAGCTTGATTATTATGAAGATGGTAATTTACAAAAAAAAGCTGAAAAAACGAATTTACTTTTTCTAAGTCCACCTTACAAAGAATGAGATGATATCAAACAAAAATGAACTGGAAATCGTTATGATTTTCTTTATGAACCTTCGAAGTTAGTTTCTTTTTACAGATCAATGATTTTAATAACTGGTTCAGCTAGTGAAATTACAGCTATTAAAAAAGCATGAAATGAAAAAAACTGAAATCAGTTTATGAAATTTGGAATTGGTCATGGACAAACAAATTCAGCTTCACGTTTTGAGCTACCTGATCTTTTATTTAGAAAACATTTTGCTAAAAATTATCCCGGATTACAAAATGCAATTAATTCTGATCCCGATAAATTTGCCGTAGTTAGAGGAAGAGAGATAGGTATAAATAAAAACATCAAGATTGTTTTTGATGATGCTAATTCATTTTCTTGAACACAAAATATTAAAGGTTCAAAAAGACCTTTTTACACTCCAATTGATCCTAACGATAGATTAGAAATTCTCACTTATAGTGATCCGCTTTTGTATGACATTGGTATTGTTAGCAACAATTTATCAAGGATATATCAAAAAGCTATTGGTGAAATTTTTATTGAGTTAGCGCAATCAAATGAAGATCTATATGGGCCTTCAATTGGTTATAACGGCTATAAAATGATTAATGATTTTCAGAAAGAAGTTGTTGAAATAATTGAAAAAACCTATGGAAAATAAACCAATTCTTTCTTTTGAAAAAGTATCAATAATATACAAAAAAGCTCCACTTTTGCAAAACATTAGTTTTAAAGTAATGGCAAAGGAAAATGTTTGCTTATTAGGTAAGTCTGGAGTTGGAAAATCAAGCCTTTTAAACAGTGTTACTAATACGAAAATAGTTAAAAGTGGGTTAGTTTACTTTGATGGTGTTGCTTCAAACAAAAAGGAATACAAAAAACTGAAAAAACAGTGCAGTTATCTGGATCAAATACCAAATTTAATTGACACTGATTATGTATATGAAGCAATTTTAAGATCTGCTAAACAAAAATTAACTTGATTACAAAAATTAATTTGTTTTGAACCTAAATGAATTAAAGATAAGATCTTAGCAATACTAAAAGAAGTTAATCTTAATGATTATGTTAGTTACATTATTAAAGATCTTTCTGCTGGACAAAAACAAAGAGTTGAAATTGCTAAGCTTTTTTTTAAATCACCAAAGCTACTTTTAGTTGATGAACCAACCACAGGATTAGATCCTTTAACCGCTTCTAAAATAATGGATTTAATTACTGATTTTGTGAAAAGAGAAAAAATAACTTTGGTTTTTGTAACACATGATATAGATTTAGCACTGAAATATAGCACCAGAATTATAGCACTTAAAAATCATGCTTTAGTGTTAGATAAATTAACAGAAAAACTAACAAAAGAACAACTTTATAAAATTTATGATAACTAAGTTGTTTTTTCACCAAGTTGGCGATAATAAAAAACGCTTAATTTGGTATTGAAAACTATTAATGATAATTGCTGTTTTGGCTATTGTCATTTACAGTTGAATAGATAACTTTTCTAGCTTTAATCAGTTTGGTTTAAATGTTTTTATTAATAACATTACAAGCTTATTTACCCCCAATCTTAGCCACGAATATACACTAGTAAGATTCTTAGCACAAACTGCTTTTTTTGTTACGGGAGGTAGCTTTTTAGGATTTATTTTTGCGGTCCTATTTTCCTATTGAACTGCATTTAAAATTCAACCTTTTTACATTGCTCTACCGATCAGGTTAATAACTATAGTTTTAAGAGCATTTCCCGTACTTTTATTTGGTTTTTTATTTAGTAATTTATTTAATAAACAACTAGCAGCTACACTAACGATTAGCTGGTTTAGCTTTTTATGGAATACAAAATACATTACTACATTTTTTGAAAACAGCAATTTAAAGTATTTTTTTAACCAAAAAATTAGAGAAGGAAGTGGTTTTAAAGCTTTTTGAACCACTATTTTTCTTAGTGAAAATGAGCGACTATGGTTGTTTTTTCTTTATAGCTTAGAAGCAAATTTTCGTTGAACTACGCTTCTAAGTATATTTGGCATTGGTGGCATAGGACAACTAATTGTTAATCCTTTAAGTATAAGGGTGCAGTTTGATCTTGTATTAATTCCATTAGTTGTTTTAATAACCTTTCTAATTTTTATAGAAGTTGTTGTTTTTTTATTATCAAGTTTTATTTTTGAGAAAAATAGTGAAGATTTAAGACCAATTTTAAAAACAACAGTTATTGAAAAACGAAAGTGAAAAAGAATTATATTTATTTTGTTTATTGTAGTACTAATTAGTCTTAGTTTAGCCAATTTAGTGACTATTGATTACAGAATTAACGATGCTGAATTTTTACAAGATTTTTTTAACCACTTTTTTCAGTTAAAAAGTAATCTTTTTTCAAGTAATGATCCTAATATAAACCCAATTTTAATGTTAGTTAAACTTACAACTCAAGCTATTAGTTTAATTAGCTTGGTTGTAATCTTTTCTATTCTCTTTGGTTTTATATCATGTAATTTATTTAAAAAGAGATTTTCAATTAGTTTTAAGATCTTATTACTTTTTGTTAGAGTAGTTCCTAGTATTCTGTTGTTTAGGTTACTAGATCCTTTATTTCTAGAAGCAAAAACAACTATTATTTTAGTCTTACTAATTAATCATGGTTCAAGTTATGGCCAATTGATGGCAATTAACTTTAATAAGGCAAATCAAAATATCATCAATAACTATAAAAATCATGGTATGACAAAAGATTTTATTTTATGAAACTATTTGTTAGTTGAAAATAAACCTAATTTAATAAACATTACCAGTGATGCTTATGATAGTGGAATTAGGGATTTAATTTTGTTTGGTAGTTTTGGCGGTTCAATTATTGGTAGTAGAATTACTAATTTTTTTGAAAGAGCTCAATTTGATAATCTAGGTTCTGTTACAATCCCATTAATGGTTTATCTAATTGCAATAGAAATAATTTTTCTTTCAGTTAGATTAACTAGAATTTCAGTTTTTAAGAACTACCTTTACTAATGATAGTTTTGAAAGAAGCGCTCTAATATTAAAACTGCAGCTAATGTATCTTTAGCCTTTTTAAAATCCTTATGTTTTAAATCCATTGTTATAAGCTTATCTTTAACAGCACTTGTAGTATTACTTTCATCAACCAAAATTATTGGCAAATTAAAACGTTGTTCTAAAAGTTGTTTAAATGATTTAATTGCCTTTTGAATATCAGAATAATAGTGAAATTTGGGAAAACCTATCACTATTTTTTCAAGTTCATACCCATCGTTTTTAATTCGCAAAAATAAGTTGTTAACAGCAGTTTTGAAATTATTTTTTACTTCAAAAACATGAAATGCAGAAGGATATTTATCTAGTGTATTTGCAATTGCTGTACCTATTTTTTTCAAGCCAAAATCAATTGCTAATATATATTTCACTACTTATTTTTGAAACAATTCATTAAGCTTTTGAGGGGTAACATTATCCTGAAAAGAACCTCTAAATAACTTATCATTGCCTCCGCCCTTTAAATTAAAACTATTTCTTAATTTTTCAATAATGGTGGTAATTTTATTTCCTATAACAATAAATGAATTACTTCCATTGAATTGATTAATTATCAAGAAATTTTTAGTTTGATTTTGATTGAAAACATCATGTATTGTTTGTAGCAATAGTTTAGGTTCTACGTCATTAAAAGTAGCAATTACATAACTTTTATTTTTATCTACTAAGGATAATAATTGCTTTTTAATTGATAAGGCTAAAGCTTGCTGTGATACTTTATAGTTTTTTGTTTTTAACTGGTTAATATCATTTTTTAAAGCTAATAAAGTATCAGATGCATCTCTTAATTGGGTAATTTTTTCAGGTAAGATAAATTTATCTAGCCTTTGTTGCAATTCTTTTAACTCAACTTTAAAAATTGAACTATCAATCAAAGATAGAACTTTTTCAAGTTCTGATTTTAATTGGATTAATTTTTGATTTTCTGCTTTTAAATAGTTGTTAATAGTTTCATTACTGCTAATGATTTCAATCCTTCATCTTCCAGCTCCTAAAGAATAGAAATCAGTAATAAAACAATCTTCAATTGAAGCTGTGTTAGCTACATGAGTGCCACCACACAACTCAACACTATAATCACCAAAGCGGATCACTCTTAATATTTCATGTTGAGAATATTCCTCTTCAAAATAAGCAATTGCATTTAGTTTTTGACTCCCTTCAAAATCAGTAAAAATCTCTTCTGAGCTTATTTTTTGTTTAATCAAAGAGCGAATTTTATTTTCTACTTTCTCAAGTTCATTTCTTGTTAAATGACGATTCAAATTAAAGTCAATAGTTGCTTTTTGCGCAGATTTAAAAGCACCACTTTGTTTAATAAGTGGATCAATTTCTTTTTGTAAAGCTGCATGTAAAAGGTGTTCTAAACTATGGTTGTTAGCAGCAAGTTTTCTTCAAGTTTCATCATGTGATAAAGTTACTTGATCATTGAGTTTAAAACTACCTACTAAAAAGTAGTGGAAGTGTTGTTTATTAGGTCCTTTAAATACACCTTGAAAACTGATTTTTTGATCATTATTATTAGAATGATTAATGCAACTTCCTTCATCATATCTCTGTCCTCCAGAAGTAGCATATAAAACTGTTTGGTCAAAAACTACATAACCACTTTGATTATTAAGTTCTTTAACTGGTAAATAATTTTCATCAAAAAGACCAATTACCTTAGCATTAATTTTATTTTTGTGATAAAAAAAAGTACTTTTAGTTTTGAAATTAACTAAATTAATATTTTGTTTTTCAAAATTTATAGTTTGGTTATTTTGCTTAGAGATAGAACGATGTTTGGCCATTAACTGATCAAATACTGTTCAATCAATAGTTAAACCTTTTTGATTAACTAGTTCTCTTATTATTTCAACAGGAAAACCGTATGTTTCGTTCAATTGAAATGCTAATTGGGGAGTTAGAGTATTGTTTTTAACACTTTTTTCAAACAGCACTAAACCTAAATCAATCGTTTTATTAAATGCATTAATCTCTTTTAAAACTACCTGTTTAACAGTTTCATTTTTAGCTTTTAAATGTTGATAATAATTCTCATATGAAGCGATGATTTCATCTATTATCTTTTCAATAAATGCTAAGTTTAATTGCAATTTCTTACAAGCTATTAAAGCACGTCTTAAAAGTCTTCTTACTACATAATTTCTCTCATTAGGACCAGGTAAAACTCCTTCTGAAATGGTAAAAGTGATTGCTTTAAAGTGATCAGCAATAATCCGAAAATAACTCTGTTCTTTCACTTTTTGAGGATCGAATGTAAAGTAAGAGTTGGGATCATATTTAAATGGACAAAAAGCTTCAATTATTTTGATTAGCTTTAAAAAGATGTCAGTATCAAAATTGGTTGGACTATTTTGTAATACTGAAACAAGTCTTTCTATTCCAGCACCTGTATCAATATTTTTTTGAGCAAGTTCTGTATAGTTGCCATTACCATCATTATTAAATTGACTAAAAACAATGTTTCATATCTCAACATAACGATCATTTTCAATGTCCTCAAAGAAAAGTTTTTCACCAATTTTTTTAGGATCAAATTTCTCACCACGATCATAATAGATTTCAGTGCAAGGTCCACAAGGTCCTAAACCTAAGTCTCAAAAGTTACGACTTTTGTCACACTTAATAATGTGATTTTTATCAATTTTATGCTTAATTCATCTTTTATAAGTAGTTTCATCATCTTCATAAACAGTGATATAAAAACGCTTAGGATCTAGCTGATAATAATTAACTAAAAGATCAAAAGCAAAATCAATTGCTTCTGTTTTAAAATAATCACCGATTGAAAAATTTCCAAGCATCTCAAACAAAGTTTGATGTCTTGAAGTAAAACCCACATTTTCAATATCATTTACCCTTAAACATATCTGTGCATTAACAAGGCGTTTAGATGGTGGTGTTTTTCTAGCACTGAAATAATCTTTTAAAGTAGCAACTCCTGAATTGATTCATAATAATGATGGGTCGTTGATCGGAATTAGTGATTTTGAAGCTAAAACCAGATGGTCTTTCTTTGCAAAATAATCTAATCAGGTTTGCCTTACTTTATCAGTTGTTCAATTCATTGCTTAATTTTAAATGCAATTTACCAGTGATAAAAATAACTATAGTTCTTTTATTTGCTAATTTATTTTTATTTCAAACTTTTTGTTAGCAATTTGTGCATAAACATGATTATCAGCTAAGAACTGCTGAAACTTATTTTGACTGTTTACTGTTCATAAATTTAAAGGTAAGTTAAGTTTTTTGATCATTTGGGGATACTTTTCATATATTTTGGTTCATGGGTGGAGGAATTGACAAATCTTTTGGATTCTAGCTGTACTAATTGTTTCAAACTGTTTTTTAGTCCAAAATAAAAAACCTTTTTTGTAACTATTATCTAAATCATAAACTTTTTGCAAAGATTCAAAGTTAAAGGATGAAAACAAGATCTTATCTATTGCTTTTTTACCATAACCTTTAACTAGGTCAACAAGCTTCTTTTCAATTCCTAAATATGGTTTTTGATCAGTTTTAATCTCAATATTGATTAACTTAAATTTATCTAAATAAAGATCTAAGAACTCTTTTAAAGTTAGGATCGATTGAAATTGAATTTTAAGGTGAAAAAAAGCACTATGATCATCTCTTTTTAAACTAACTAATGATTCAAACTCAACCTCCTTATTAACTAATGCGGTTCTCAATGTTGTCTCATCATGAATGATAACTAACTGTTCATCTTTAGTTAAATGAACATCAAGCTCTATTCCATCAAAACAATATTCAAAAGCTAAATCAAATGCTAGTTTGGTGTTTTCTGGAGCAATGAATGAATAACCCCTATGTGCTAAAAGCAATTGCTTGTTATGCATTATCAAGATCTCTTCAACGGTATGAAGCTTTATTTAATCGTTTAATTTCCATACTATTTGAGATAAAAACAATTGATCCACATATCAAACCAAACAAAGCAATGGATAAACAAATAGCAGCGATAATTTGATAACCAGTGTTACTAGTATTACTTTGTCCTGCAACTGTATATTTCTTACCAACTTCAGCAGTCACAGTGTAAAGTCATCCATCAGTAGAAAAACCAATAAAACTAAGTAAACCAACACTAGATGCATAGTTATTTTTACCAATATCAATTTCACCTATTTGGTTATATCTGACTGTAACCATTCCCCATGATAAGATACCAGTAAAGATATAGAGAATGGAAGAGAAAGTAATTAAAGTGATGTTAGCAGAATTTGTTTGTACAAAACCAAGTAGAATAAATGCTAGAACAAATACAATACCAAGAACAGTGCATATCATTAAAAACAAGATATAACTTTTACACTTATCTGCTAATCTACCAAGATAAACACTAACAGCACTTCTCAGTGCATAAGTTCTAATTCCCCCAATTACAGTTACTAATACTACAGGTGCTAAAAATGCATTTTGCAACATCTGTAAAAGGTAATAAGCAAAAGTGCTTTGAAACACATACATCCCCATTAAAAAGAAGGATAGTAACCATAACTTTCAGTTTTTTAAGGTCACTAAAATCTGGTTTAAATTGCGTTTAAAACTTACTAAAGTGGTTTGACTTTGTTTTTTAATTGGCTTTTCTTTAACAAAAAACAAAACTGTAAATCCAGTAATTACTAGCATTATGGCAATGATAAAAGCGTATGCAGCAAATGGTTTTGAATCAGCATTCTCACTACCTCCTGATGGATAGAAAATGCTGGTAATTATCAATGCAATTAGAAAGATAAATATTAAGCCTCATATTCCATTAGCAGCTCCCTGAATTCCAAAACCAAGTGCTTGATTTTCTTTTGTTGCTTGTTGACTAGCTAGTTTTCATAATGGTGTTCAAAAGATTAAAGTACTTGTTATCCCTCATAACCCTCATATTACACAATACTGAATAAATAAAGCATCATGGCTTTGTTGGTTTTTAGTTAAGATGTTGGCTGCTAATCAAGAAGTAATTGCTCCAGTAGTAATTGCTGATAGAAATAACAATTTTCTAGAACTGAAGCGATTGGCTAAAAACCCTCCTGGTAGTTGAGTGGCAAGCGTTACATAACCAATGATAGAAGTAACAGTAGCAACTTCATCCTCTGTAATTCCTAAGTACAAGTGGAGGTTAGGAACAACATTCTTAACATAATAGGGTGCAGCAATTACAAATACATCAATTGCTCCCAAAATAATTAAGGCAAGAATTTGTTGTTTTGAAAAATCCTTTAAGCGTTTTTTAAATCCTTTTTTTGTTGTCACTATCACTAATGATAAAGTGGGATAAATTAAATATACATCTAATAATAAAAGTGGATGGAAGTGAAACGTTCTTCACTTACTTTTAAAAAGAAAAATAATGTTTACTAGAAAAACTATTTTTTCTTAAATTAATCCTGCTCCTAAACAAATTTGATCAAGTGTATATAATACACCAAACTGTCCAGATGCAACACTTATAACAGGATTTTTAAATGTTATTTCCAGTTTATTATCTGATAATAATTTCAACTTTGCAATTTCGGGTTTTTGAGCATGTCTAATTCTTACCAGAACTTGACTAGGAAGTTGCTTTGGTGTATACAACCAGTTAAATTGATCCAATAAAATTGTTGTTTTCAATAATTCTTCTTTGTCACAAGAAACAAATAATTCATTAGTTTCAATATCCTTAGCAACAACAAAATGACGTTGTTTTAACCCCCCTAAATTTAATCCACTGCGTTGACCAATCGTATAAAACCAAACACCATCATGTTCACTAATAGTTTGTTTGGTTTTTCAATCCTTAATTAATCCTTTTTTTACAGGTAAATAGTTTTTTAAAAAATCACTGAAATGTCTTTCACCAATAAAACAAATTCCAGTTGAATCTTTTTTATCTGCAACTTCTCAATTATTTTCTCTAGCAATATTTCTCACCGTTATTTTTTTTAAATCTGCTAAAGGAAAAATAACATTCTGAAATTGTTCTTTTTTAACATTTGCTAAAAAATAAGTTTGATCTTTATTGGTATCTTTAGGAATAGAAAGCAAAGGCTGATTTTCTATCATGTTTATTTTGGCATAATGACCAGTTGCAAAAAGAGAATTAGGGTTAATTTGCTTACAAAAATCATGCAATAAACCAAACTTAATAAAACGATTACACCAGATGTCTGGATTTGGGGTTAACCCTTTTTTGAAACTTTGAATCATAGGTAAAAAAACTTTGTTTCAATAAGCTTCAATTAAGTTTTTTTTATTAACTTAATTCCTAAAGAATTAGCGATTTTTTTAGCCTGTTGGAGGTCTTGAAAAGATGAACAACCTGATTTGTTATTATTTATTTTCTTATGACCATAAAAATCATTATTAAGTGTCTCATCCCAACATTCCATAAAAACACCAATAACTTCTTGGTATTGCTTTTTTAAAAGTAAAGCACTAACAGCAGAATCAACACCACCACTTAAACCTATAAAAACTGTTTTTGCAATAATTGACATTTTTAATAAACAGCGTGAATAGCAAACTTGTTTACTAATCTTTGGTGAAATCTATTAGGATTAATGCCTTTTGTAATAGCAAGTGCAGAAATAAACACTATTAATGCTTCATAAAAATTTTGACAGCACTGCTGATCACTATCTTTAGGATTTTCTACAACTTTTGCTAGATAATCTAGCTGTTTAATTACTTCAGTTTGGATAAAAAAACCATCACTTAACATTGGTTTGAAAAAAAATGGTTGGTCATCATAATCATCATCTTCATAAATAACAATATTGTCTTGTTCTCGTTTTAAACACAAATTAAAGTTAATGCGAAAAGTCTCAAAATCAACCGCTTGAAATTCAGTTTGTAAAAATTTTTTAAAAGCTATGAGTTGTGGTTTCATTACACCAGTCTAAAAAGTTTTAACACTTCTTGTTCAAGATAGCAATCCTTAAACAGTTTATTTCAGTATCACCTAAATAAACTACTAACCAAAATACTGTAAAAGGTTTAATTTAAAAACTAATTTTCTTTATTATCTAAGTTTAAGTTATTAAGTAAACCTAAAACATATTTTTCCAGATCAAAGATAGCTAAATCACTAGTTTTTTCACCAAAACCTATCAGTTTAACAGGCAGGTTAAACATATCTTTAATAGCTAAAATAATTCCACCCTTAGCAGAACCATCCATTTTAGTTAAAACAATCCCTGTTAGTTTGGAAAATTCATTAAATACCTTTGCTTGTGATAAACCTGTTTGACCTACTGTACCATCTAAAACTAAAAGTGTTTCACTAGGTTCACTTCCACTTACCTTTTGAATAATTTGATAAATTTTTTGCAATTCATTCATTAAGTTAAGCTTGTTTTGCAATCTTCCTGATGTATCACATAAAACAAAGTCATATTTATCATCAATCCCTTTCTTTACGCCACGAAAGATTACAGCTGGAGTTTGTTCTTTAGGGTTTGGAAGTATAATGTCACAGTTTAACCGCTTTGCTCACTGATTAAGTTGTTCAATGGCTCCTGCTCTAAAAGTATCACCTGCAACAAGTAGAACACGTTTATTTTGTTTTATGAAAAAATCCGCTATCTTAGCTAAAGTAGTTGTTTTACCAACTCCATTAACACCAACAAAGAGATAAACATTTGTAAAGTTAGGTTTAACTATTAAATCAGTATCAAAGAGTTTATCTTGGATGTAATAAACAATGATTTGGTCAATAATTAGCTCTTTAATGAGTTGAAAATCTGTAATTCTGTTTAGCTTGATCTGTTCAATAATAGCATCACAAATTTTGTTTGCAGCATGATAACCAACATCAAGCAAAACTAACCCTTCAAATAGATTTTCTTTAAACTGTTCGTCAACATTAACATATCGTTTTGACAGTTCATTAATAGTTTTAGCGAAAGTTGTAGCAGATTTTTTCAAACCCTGATAGTAAGTTTTATTATTGGTTTGAAAAAGGCTTTGTTTTTCAACTTCTTCTTTAAGCTGTTTAGCAACTGATTTTTTTGGTTTTAGTTTTGCAATTAACTTGCTTAAAAAGCCCATTATTAGTTAATTAGAATCATTTTCAGATACATATTTTTCAGCATTTTCAAGTTCAACTGCAAAGGTTTTAGTTACACCTTTAGTTTGCATTGCAGCACCCAATAACATGTCACATTTCATCATTGTACCTTGACGATGGGTAATAATTAAAAATTGGGTGTTTTTGCTAGCAGTTTTAATGATGTTAGCAAAGCGCTCCACATTAGCAGGATCAAGTGCACTTTCCGCTTCATCTAAAATAACAAGTGGAAAAGCACTTACTTTCAAAATACTAAATAAAACAGAAAGTGCAACTAAAGTTTTTTCCCCGCCTGATAACAACATTAAGTTGGCAATATTTTTACCAGGAGGATTTGCAAAGACATCTATCCCTGAAACCAATACATTACTAGGATCAGTGTAACGAATTTGGCAAGAACCACCACCAAATAGATATTTGAAAGTTTTTGGCAACTCTTGATTTAATTTTTGGATCAGTTGATCAAACTCATTGCTAGCAATTTCATCAATTTCAGTAATAGCTTTTTGCAAATTTTCAACTGCTTGTTGAGCTGATTCATATTCAGCGTTAATATCATCAAAGCGCTTTTGCTTTTCACTTATCTCAGCAATTGATTCCATGTTAATTACCCCCATTTCATTCAATTGATTTTGTAGTTTAGCAATCTTGTTATGGGCTTGCATAAAAGAGAGTTTAACTGGTTTGTTGTGATTAGCAATAGCAAACTCCATTGTCATTTTGTAAACACTGTTAATCTTTTCAGTGATATTTTGGATTGTATTTTCAAACCTAATTTTTCCTTCCCGCGCTGAAACTAATTTAGCACGTTGTTCATCTAACAATGCCCTAAGATCAACTATCTTCTCTTCACTTTGTTTAATTGTTTTTGCTAGTTGTAACTTAAGTTCTTGATTAAGCTTTAACTTAGAGTTAATTTCATCACGTTTAGCTCAAGCACTATTTAATGAATGGATTAATTCAGCTTCACTAGCAACAGCTTTTTTACCATCAAAAGTTGTGTTAGTAAGCTGTTCATATTCCATTTTGAGCTCTAAAATAATTTTTTTAATTTGAACAATACGCTCTATAAACTTGGCTTGTAACAACTCTTCATACTTGAGTTTTCTGTTCATTTCATCTAGCTTTACTTCTAATTCTGTAAGCTCTTTTTTTAACTCTCGTTCGTTGTTTTCTAGTTTGTTAATGTTCTGTTCATTATCAAGTGAAGCTGATGATAAATACCCATCACTAAGATTTGTTTTTTCAAATCCACCATTGATAATTCCCCCTGCATAAACTGTCTCACCATCTAAAGTAACGATCCTATATAACTTGTAAGTATAGTTAGAAAGGTTAATGGCACTATTTAAATCTTTAGCAATAATAACTTGTGCTAAAAGAGTATTTACAACTGGTTGAAACAATGGATCACACTTAACATGATCACTACAAACACCAAGAAAACCATCAAGCTGCTTTAAAATTTCCATGTGTTCGTTTGTGATTTTAGTATCACTAGCAACATCATCTAAAGGTAAAAAAGTTACTTTACCTATCTCATTTTTGACTAAAAAATCTATTGCTTGGATGGCAGCATTATTGTTGTTAACAACTAGGTAACCAATTGATTTTCCAAGTGCTTTTAAGATAGCTTTTTCATACTGCTTATCAAATTTAAGAAATGTTCCTAGTGTATTTAAAATTCCTGTTAAAGCATTAGCATTTTTAACTAAGATATTGGCATTGTTAGTTTTTTTAAGCTCATTAGTTTGGAGTTCAATAATTGTTTTTAAGGAACGTTGGTAAATTAAATCAGCAGTATTTTTTTCAATCTGTAACTTTATCTGATCAACAAGTGATTTTTGTTCGTTAATTAGTTTTTCAAGATCAGTGATGGTTGTTTTGGAATTTGATAGTTGGTTTTCAAAACCATCAAGCTGGGTTTGATCAACTAAAATTAGTTTTTTTAAAGCTGCAGCTTTTTGCTTTTCATCTTTTTGTGAAAAACCTTGTCTTAACTGCACATCAATAATGACTTTACGTTGTTCCAATTCATTGATCTTTTGGTAAATATCTTGTAACTCTTTTTGCAATTCATTAGATTGCATATCAGCACTATGAAAGCGACTATTAAAGATAACAATCTGTTCTTCTAACAACTCTAGTTGGGGTTCGTGTATTTTAAAGTCATGTTCACTACTGTTAATCTGAAAATTAAACTTATCAAGTTCAGTTTGTGCTTGGAGATATTCCCCAACCAAAACAGCAAGTTCCAACTCTTTCAATTCGTTTTTAACCCTAATGAATTGTTGTGCTTTTTCAGCTTGTAATGTAAGCTTTTTTAAATCTTTTTTCAGTTCATTTAAAACAACGCTAACTTGTTTTAAGTTAATTAAGGTTCGATTTAACTGGTTAACAACTTCTTCTTTACGCTTGGTATATCTACCAATTCCCGACGCATCTTCAAAGATCTTTCTGCGTTCTTCTGGCTTAGCTTCTACAAATCAAGAGACACTCCCTTGTGAAATAATTCCAAGTGAACCTTTTTCAAGACCAATATCAGCAAAAATACCGCTAATTTCTTTTAAAGTTGCTGGATTGGAGTTAATAAAATATTCACTCTGGCCACTCCCTCTATAAACCCTACGCATCACGCTTATCTCTTTTCTTGAATCATGTAATAAACGGTTGGAATTATCAAAGGTTAGCTCTATTTCAGCTAACTTACTAGCAGGTTTGTCTTTAGAGCCAAAAAAGATCATATCATCACCTGATTTACTACGGAGATGTTTCATACTTCTCTCCCCTAAAACCCACTTTAATGCATCAACAACATTGGATTTGCCCGATCCATTAGGACCAACAATACCAGTCATTGAATGGGTAAAATCAATTGTGATTTCATCAGCATATGATTTAAAACCATATGCGCGAAAACGTTTTAGAAAAACCATTTGTTCTAAAAAGCTTTAATTTGGATGTTGTTATTAGATTATACTAGTTGAAGTAAACTAAACTAGGCTAATTATTAGGTTACTAAAAAATAAGCTAATAACGCTTCATTTAAAAAAGCTTCTATGTTTTAATAGTAACTAGTTTATTTAATTTAAAAATTACATTAATTGCTTTTAGAAAAGCAATATCATTTAAAAGTTTTTATTTAATTGCTTGAGCGGCAGTGATAATTCCAACATTAAAAATGTCACTGACACTAGCTCCCCTTGAAAGATCATTCACTGGACTTGAAAGTCCAAGAACAATAGGACCAATTGCATCATACCCTCCAAGTCTTTGGGCGATTTTATAAGCAATGTTACCAGCATCTAAATTAGGAAAAACATAGATATTAGCACTATTTTTTCAAGTTAGTTGAGGTGCTTTTTGTAACCTAACCTTTTCAACAAAAGCAGCATCAAACTGGAGCTCACCACAAACACTTTGATGCAATTCAGGGTGTTTTTCTAAAAATAGTTTAGTTGCTAAAACAACTTTATCCACCATTTCACCCTTACCACTGCCAAGCGTTGAATAGCTTAAAAAAGCCATTTTTATCTCATCCTCATTTAAACTTTTGGCAAAATTGAAGGTGTTTTCAGCAATTGTTGCTAACTCTTGGGGGTTAGGATAAACAGCAAAAGCACAATCAGTGAAGTACAAACGTTCTTCACCTTTTTCCATGATGAAAACACTAGAAACAAAATTACCAGTTGCTAGTAACTGTAAAGCTGGTCTTAAAGTATCTTTTGTAGCATATTCTTTACCACAAACCTCACCATCAACAACCTTTAGAGCAACTAAGGTAGCAGCTAAAGAACTAGGATCACGTACAAACTTTTGTGCTTCTTTTAAATCCATCCCCTTATGCTTACGTTTTTCATAGACAAAGTTAGCATAGCTAGTTAAATCCATCTCATCAATCACATAATGAGTTATTTTTTTATCAAAATTTGCAGGGATTTCCTGACGATTATGAAAGATAACTGCAGGTTGGATCAGCTTAGATTCATTAAGCATTTCAACTGCTTTTAAAACACTTGCTGATCAACCTTCTGGAAAGATAATTACAGGTTTTTTACTAACAGCTTGTAATCGTTTTTTAAAAATATCAATAACACTCATTGTTTTATTAATTTGGTTTAAGAATTTTTTTGAATATCGCTAATACCTACTAACTCTTCCCCACCAATTAGTGCTAAAGAAGCACCACCACCAGTGGAGATAAAACTAAACTGATCAGATAGTTGCATTTGCTTAACTGCTGCAGCTGAATCCCCACCACCAATAACGCTAAAAGCAGTTTTATTTTTAGCAATAATCTCACCGATTTTTGAAGTTCCTTTAGCAAAGTTAGTAAATTCAAAAACTCCAAGGGGACCGTTTCAAAAGATAGTTTTGGCTGTTTTTAAATAACTTTCAAATAAAGCAATTGTTTTAGATCCAACATCTAGAGACATATAGGATTGATATTGTTCTTGAATTTTGTCACTAACATCTAAAGTAATGCCAGCTTGATCTTTAAATTCAGAACCCATTACCTGATCAATTGCCAGCACAATCTTATTATGAGTATCTTTATCCAAGATTTGCTTAGCAACATCAATTAACTCTTTTTCAACTAGGGAATTAGCAGTAGCTTTGCCTTTTGCTTTAAGAAAGGTATTTACCATCCCTCCGCCAATTAAGATATTATCAGCAAGTTTTAGTAAGTTTTCAACTACCTTTAGTTTATCTGATACTTTCGCACCACCCAAAACAACAACAAAGGGTTTTTGTGGGCTTTGAATTAGGTAAGAGAGGTTCTTTAGTTCTTTTTCCATTAAAAACCCAATACAGGATTTTGCAACATACTTTGCAATTCCTGCATTAGAAGCATGTTTTCTATGGGCAGTACCAAATGCATCATTAACAAAAATTTCCCCTAAACTAGCCCAGAATTTCGCTAGTTCAGGATCATTTTTACTTTCTAATTTAACAATTTCTCCTTTATCGTTTACATCACAATAGCGAGTGTTTTCAAGGAGAAGGATTTCACCGAATGCTAATGCTTGCACTTTTTGTTTAACTTCAGCACCAGTGTTTTTACAAGAAAATTGAACCTTTACAGTTGGTAAGAGTTGTTGGAGTAATTCAGCAACTGGCTTTAAAGATTTTTTGTTGTTTAGTTTATCTTCTAAACTCTTAATCCTTGAAAGGTGTGATAGTAGCACTATCTTGCAGTTCTTTTTAACTAAGAACTTAATAGTATCCAAACCAGCTAAAATTCTTTCACTATCACTAATAACCCCATTGATCATTGGGACATTAAAATCACTTCTTAAAACAACGGTTTTGTTTTGAAAATCAATTGCTTGGAGTGTTTTGAAATTAAGCATATTAGAGCTTAGCACAATAGCTAACTACTCTCACTAGTTGGTGTACATAGGAAGATTCATTATCATACCATGCATACACCTTATAAAGTTTCATGCCATCAACTTCAACAATATTGGTTAGTTTAGAATCGAAAATTGAACCATATTCAGAACTTACCACATCGCTAGATACAATAGGATCTTCACAATATTTAAAAGAAGCGGAAGCAAATCGCTTCATGGCTTGATTTACTTGTTCAACAGATGGACTTTTTTCAAGTACAACACTTAACTCTACAATAGAACCAGTTAACACTGGAACACGGAGTGACATCCCATTAAGTTTGCCATTTGCTTCTGGAACAACAAGCCCAATTGCTTTAGCTGCTCCTGTTGTTGTTGGCACAATGTTAACAGCTGCAGCACGAGCACGACGTAAGTCATTATGAGGAGCATCTTGTAAGCGTTGATCTGCAGTATATGCATGAACTGTTAGCATCGTTCCATAAACAATCCCAAAGTTCTTTTCAAGTACATGAACTAATGGTGCTAAACAGTTAGTAGTACAGCTAGCTGCTGAGATGATCTTATCATCGCTACTAATGGTTTTGTGATTAACATTGTAAACAACTGTCCTGATAGTTTTTTCTTTAGCGGGTGCGGAAATAATTACTCTTTTAGCACCTGCTTTTAAATGGAGAGAAGCACCCTCTTCACTTACAAACCTACCAGTTGATTCAATTACTACATCAATATCATGTTCATCCCAAGGTAAATTTTGGGGATCTTTTTCACTAAAAACATAAACCTTTTTTCTATCAATTTGCAAGATGTTTTGTTTAACAGTAATCTTTCTTTTCAATTCACCATGAGCTGAATCATATTTCAACAGGTGCGCTAAAACTTCAGGTTGGGTCAAATCATTAATTGCTACAACTTCAACATTTGCCTTACTGAGAAGAGAACGAAAAACAAGTCTTCCAATTCTTCCAAAACCATTGATTGCAACCTTAATGGTTCTATTCTTTGCTGCCATACTTTAATTTAATAATTAGATTTTAATACTTTAGCCTATTGTAATTAATTCTATCTGTCAAAAATACAGCAAATTATTGCTATTAATTGCTCTTAAAAAAATAAAGTTTTGCTTATCAAAATTGAAACTATAATTAGGATTTTCTCAGTAAACTAAATTCCAGTTATTAGCAATTAATAAAATACTAAATAAAAGTAAACCCACTAATAAAAATACCCCGTTTAATAAGTTGTATTTTAAAGGATAATGAGTTCTGTTAGTGTTATTTAAATCATAACCTTTAGCTTGTAGAGCAAACGCTGTTGTTTCTGTTTTTTTAACTGTTGAAAGTAATACGGGAATAAAAAGGGTTTTAATCTTGAAAGGATTTAAAAACGAACACTTGTTATAGATAAAACCTCTAGTAGCTTGAGCTTGTTTAATTCTTATAATTTCACTTTTAACAGTTGGGAGTAATTTAAATATAACTGCAAGCAAGATACTAATTGGTTGAACCTTAATATGAAATAACTTAAGAAACTTAAAAAATCTCTCAACCGCTCAAGCCAATTCATAGATAGAACTTGAAGTTGTCAGTAAAAAAGAAGCACTGAAAAGCATACTAATCCTTAATGCAATTACAAAGGATCGTAAAAATGAACGTAGATTAAAACTTCACCAGCTAACACCACAAAAATTATTACCACCTGAATAGATAAAACTTCCTAAAAAATTATGGTTCTGATCAACGCTTAAAGCAGTATTTGGTAAAAAGATAAAACCGTTAACAATTACATTAAACCACAAGAAAAATAACATCCAACTCAAAATAATTAATGCACTTTTAACCCTTTTCTCACTAATTACAACTAGGGTTAAAAAAACTAAGTTAATAATTACAAGACCATATAATCCTAGCGGTAGAAAAGCAACAACTAAACTAATTAGTCAAAACCACAATTTTAGAAGTGGGTCAATTTTGTTTAAAAAACTGGTTTTTTGCATGAAAATAAGTTTTTTAGTTCACTAATAAGATCATCAAGGTTTTTAATTTCTGCTTGGTTTTTAAAAGCTATTCCCTTTTCATTAAACATCTTAACTGCTTGGATAATTACTGGAGGGGTGATTCCATATTGCATCAACCAACTGGTGTTTGAAAAAAAATCATTAACAGTGGTTTGATGAATTATCTTTCCCTGGTGGAGATGGATGATTTTATCAGCAGTTTCAAAGATAAAATCAACATCATGGCTAACAATAACAATCCTTGTTGTTTGTTTCATTGCTACTAGCATTTTGCTTAAGTTGCTAATCGCCATTTGATCTAACCCAACAGTAGGTTCATCAAGGATTAAAAACTTAACTTGCATTGCTAACACACTAGCTAATGCCAAACGCTTCTTCTGTCCATCGCTTAATTCAATGGGATTTTTTATTAACGGGATGTTTTCCAATCCACATAGATCAAGGTATTTTTTGGCATAATTAACATCTTTTTTATGACAAAGTTTAAGGTTCACTGCTCCTGTTAAAACTTCTTCTAAAATAGAATCACAAAAAAACTGATCTTCTGCTTTTTGCAAAATATAACCAATTTTTTGCTTAAAGTTTTTTGTTATCTTTTGTTGGTATTGGTTTGCAAAATAACAATAATTAGCACAATTTATATAGCCAAAACTAGGTTTTTCAAATAAACCTAACTGTTTTATTAGTGTAGTTTTACCACTACCACTTTCACCAAAAATAACTGTAATTTTATTAGGTTCTATTACCCCTGAACAACATTTAATGATCTGACCATCATTATGCTTATTGACAAAGACACTAATATTGTTAAAAAACAATGGATCTTGTTCAATGTTCAGTAATTTGCTATTAAAAAATGGAATGCTTTTTTTACTGTTTAAAAAGTAAAAAGGATTAATGATTTTATCAACCTTTTTATAAAAAGAAAACAGTTTTTTTGAATCTAATTTTGCTAGTTTAAGATTCGCTTTTTGAGCTTGAAGATGGTATTTTTGTTCAATTAAATCTAGTTTTATTTGAGGTGTTTGCATATCCAGTTAACTACATCATTCAATTCAAAACTTAAACTTGGTGTTTTTTTGTTTATTTGTTTAGCCACCTTATGGGCCAATAAAAGTGGAAAAGGGAGATTAAAGTGGTGGTTATGAAACAAATCAAGCTGCTCATATATCGCTTCAGGAGCAAACTTTTTAATTAGCGATCCTTTACTTAAAAAGATAATCTCATCTGCTAGAAACAGATCATTAAAATCATGGGTAATATTGATAACTATTTTGTTTTGTTTTTTGATGGTATCAAACACAAATTTTTTAAGTTTATTGCTGATCTTATTATCAAGCATACTAAATGCCTCATCAAGCAGATAAATTTGTGGTTTGACAGCCAATAAACAAGCAAAAACAGCACGTTGTTTTTCACCAAATGAAAGTTTTTTAAGTGGTGTAAATTGTTTATCTTTCAATTCAACAACAGTTAAAACTTCATTAATAATCTTATCCATTTCACTAGCTAAAACTCCATGGTTTTCCAAAGTAAAAATCAATTCCTGGTGGAGTGTATCAGCAAGTAATTGCACATCAGGGTCTTGTAATAAGATTCCAATCTTATTAAAACCAACAGAAGTCAACTCCTTATCATTGAAAAAGATAGTTCCTTTTTTAGCTTTTAGAAATCCGCCAAGGAGTTTAACTAGGGTTGATTTACCTGAACCGTTATGACCTATTACTGCTAAATGACACCCATCTTGAACATTAAAACTAATCTGATTTAACACATTGTTTTGGTGTTTTGGGTATTTAAAACTGAGGTTTTTAACTTGTAGCAAACTAAGCTTATTTATAAATTTTAAAAATAAGTCAGAGCTAAAAATCTGTTTTTTGGTAGATAAAAAACAAAAAAGCAGCTGTTGTGCTGCTCTTTTTTATTTGGAATATGTTCTATATGAACAGGTTATTTTGCTTGTGGACCTTGTTCACCAGTTGGGTTATATTTTTTTGCCATCTCTTCTCTTCACTTCTTAAAGTTTTCAATTTCCACTTTGAGTTTGGCATAGTCATTAGCTTTAATAGCAGCATCAATGTTACCTGTTAGCTTTTCTAACTTCTCTTTTTCTTCTTTAGGGAGATTCTTAGCATCAGGACTTGCTAATATCTCTTTGATGGTATTAACAATACCTTCCCCTTCATTACGTAATTCAATACGTTCACGGATGATGTTATCCCGTTCCTTGTTAGCTTCAGCATCACGGATCATCTTTTGGATCTCCTCCTCAGAAAGATTACCGTTGTCACTAATGGTAATACTGTTTTCCTTTTGCGTGGTTAAATCCTTAGCTTTAACATTTAAGATCCCATTGGCATCCAAACTAAAAGTAATCTCAATTTGGGGTTTACCTTTAGGTGCTGGTTGAATACCGCCTAAGTTAAATCTTCCTAATGACTTATTATCTCTAGACATTGGTCTTTCCCCTTGACATACAACCACATCAACTGATTCTTGGTTATCTTGAGCAGTTGAAAAGATTTGACTTTTACTTACTGGGATAGTTGTATTTCTCTTAATTAAAGGAGTAGCCACACCACCTAAAGTTTCAATAGAAAGGGTTAATGGAGTTACATCTAAAAGTAAAACATCCTTAACATCACCACGTAAAACCCCACCTTGAATAGCAGCGCCAATAGCAACAACTTCATCAGGATTAATAGAACGGTTTGGTTTTTTACCTGGTACCATTGATTCAACTAGCTTTTGAACTGCAGGCATCCTTGTAGAACCACCAACTAAAAGAATTTCATTAATCTCTTCAGGTTTAATCTTAGCTTCCTTGATAACATCTGAAATAGGGTTTCTTGTTCTTTCAAGTAGTGGTTTTGTTAACTCCTCAAACTTAGCACGGGTTAGTTTTAACTCAACGTTAACAGGACCTTTTTGGGTAACAGTTAAAAATGGTAAAGAAATAATCGTTTCAAGTTGAGCGGAAAGTTCAATCTTAGCACGTTCAGCTGCTTCTTTAAGCCGTTGCATTGCCATCTTATCTTTTGATAAGTTTAAACCCTGGTGTTCTTTGGCAATGTAGGCTGAGATATATTCAATGATCTTGTTATCCCAATCATCACCTCCCAAACGGTTGTCCCCAGCAGTTGCAAGTACTTCAAAAGTACCTTCTGCAATGTCAAGTAAAGATACATCAAAAGTTCCACCACCCAAGTCATAAACCAAGACTTTCATCTCTCTTGATGCTTTATCAATCCCATAAGCTAAAGCAGCAGCAGTTGGTTCGTTAATGATCCTTTCAACATTTAAACCAGCAATCTTACCTGCGGTTTTAGTAGCGTTTCTTTCCGCATCATTAAAGTATGCAGGAACAGTAATAACTGCTCTTGAAATCTTTTTACCAATCTTTTTTTCAGCAAAGTCCTTAAGATAACTAAGGATTTGCGCTGAAACTTGTTCAGGACTTAATTCCTTAGTTGTACCATCAGCATTTTGGACTTTTACTTTATTTGAGGTACCCATCAACCTCTTGATGGAGACAATGGTATTTGGGTTTGTAACCATCTGTCTTTTAGCAGCATCACCTACTATAATTTCATTGTTTTTATAGGAAACAATGGAAGGTGTTGTTCTTTTACCTTCAGGATTTTCTAATACAACAGGTCTCCCACCTTCCATTACAGAAACACAAGAATTGGTAGTTCCAAGGTCAATGCCAATAATTAAACCATTGTCTGCACTCATACTTTTTTTTAGATCAATAACAATTACTCAGTAATTATAAACAAAAATTAGCACTCAAACACTAAAAGTGCTAATTAAAGTTTGTTTTATTTATCTTTTTTAGTTTCTAAGCTAACAGTGTTAACTGTTTTCAACGGTAAATTAACGGTCTTTTTAAATTCCCATATAACTTGAACAATTCACACAACAAAACAAATCCCAGTGAAAATAGCAGGTAGATAAACAGATAAAAGTCTTCATAAAAAAACACCATCTTTAATCTGATCATGGAGGAATTGATCAGTTGGTTTGAATGCATTGAGAAAACTAGTCATCACAAATTGTGTTGCACCTTCCCCACTAGCAACTGGAATAAAGTTAGAAGCGGTAACAGCAATATTAGTAATGTTAAAAAGATCAATTAAGCTGTATTGATCAATAACGTTATTAGTTGTGTTGACAGTTTTTGTGATCATAAAAACACCAAACAAACTAAAGTAGGAAACAATTGCTACAACCATGTTAGCTAGTAATTTAAAGATTGTTAACCCTCACCTTCGCATCTCAATTCCATACAACTTATTAAATTCAGCTTTTTCTATAAAACGTTGGTAGATCTGCTCTTTAGTTAAATAAGGACGTTTAAGTCACTTTCGAAACTGGTTTACAAGTGAATAGATCAAAACATGCATCTTTTTATTGAAAGCAATAACAATAAATAAGATAGCAACAACAACATCAAAGATCATCCCAGTAAGACTTAGTCAGTAAGAGACAAAGCTATTATGGTTATTTGCTAACAATTGATAGTTTTTAGAAAGAACAAAAAAAGAAGGTCAAGTTATTAACGCTTGCGATAGATTTCAAAATGCTCCTGTAGAAGTGACAATTAAAACTGCAGTTTGTTTTTTCATCCCCTTTTTAATGAATCAATACAAGCGAAATGGATCTTGTCCTAGTGATAAGGGGGTTACAATTTGAAAAAACTGTACTACAAAACCAAATAATAACCACTCTCACCAACTAGCATAAAAACAAAATCTCCTTGATACCCACCAATTAATGATTACATTCCACAAAACTGAAACTAAAAACCCTAAGATAACAACAAATATCCAACCCCAATTCTGATAGTTAATTGCAGTGATAATAGTTTTCACATCATCAACACTAACACCTAAAAAAAAGATAGTAACAATAACACTAATAACGATTAGAAAAACCAAAAAAAAGCTAAAAGCAACGATGTTTTTAGTGTTAAAAAAGGTATTGGTGGTTAACTTTGCCATTACTTAAGCAAGTTTAAGCCTGATTGAATTCTTTTATCACCAATCCTAAATTGGGGTTTTTCAATTGCACTTAAAGCAAGTTGTTGGGTGCCTGTATCACTAGCTTGAAATAAAACTAAAGCTGCTACTATATCACTTTCAATATTGGTAGAACTTGTTAAACTAAGTGAATCAATGTCACTTTTAGTGAACTGAAGATAGTTGTAACTATCACCACCAAAGTTAAATTCACTAAATTTTTGGTTGGTTTGGGTACTTCTTTTTGAAATACTTGGACTATTTGATGAATTAAAATAACTGGAATATTGGTCTAAAACAGCTAATAACAACGCTTGTTTAGCTTTGAAATCTAAAGCAGAATTGTTAATTGCATCGACCTGAAAATAAGCTAAAAGTGTTTTGCCATTACTACTACTACTACTACTTGGAGTTAGTCTACTTAGCTGTCGGGCAATGTTATCTAAAACTAATTGGTCTTTTGTTTCACTAATAAATTTTTTAAGATCATCCTTTGAACCATATTGTTTTAAGGAATTGGCAGCGATATGGTTTAAAAGTGCCTGGTTAGTATTGTTTTCTAACATGGAACTGTTAGTTGTATTCAAACCAGTAAAACCATAAGTACCATTATTACTTGTTGATACTGCTTGTCTGGTGCTTAACATCGTAGTAGCAGGTGTAGTGTTAGGACTTGTTTTGGTTTTAAAAAAGAAATTATTAGGATCAATAGTTTGGTTGGCATTAGCTGTTGTAGCATGTTCATCATTTTCATAGTTAAAAACAGAGCCAAATATTCCCTGAGGATTATTGGTGTTGTTACTGATAATATTCTTATCATAAGCAATATAGGCAATTTCACCATACTTTAAGTTAGCTTGTAAAATATCTTTAAAGTTTTTAGGTTGTTTTCAATTAACCATTGCAAAGTAAAAATGAAGCGATAATGGTCAATAACTGCTTTAAAGTTAACATTAAAACCTGATTCATCAACACTCTCTAAAAAAGCTTTTTTAAACAATTCATCAATATTGCCATTATTAGGATTACCATACATGGCTTTATTGGTTAATTTGCTTCATGTCATGTAATAGTAATTGCTAATTACTTTAGCTAAACTATCTGCATCACTACTTGATGAAGTTGATGAAGATCCATTTTTAGATTTAATCTCTTCAAATAACTTTTTAGCTTTTTCAAACTGTTGATTGCTAGTAAAAGCATTTTTTAACAATGCAAAGGAAAGAGGGTTTACAGTTGTTACATCTAACAACGCATTTAAACTCCAATTGGCACTGTTAGTAAGTGATTGATCATTGTTAATATCAACTAGAATAATGTTGCTAAAGTTAGGTTTTGATTTTTCTGTAACTTTCAATTGGTTAACAAATTCTGTTACTTTGTTATTTATGTCGCTCAGAATTTCATCTCTTATCTTATCAACATCAGCTTTTGTAGTTTTTAACTTATTGTTGTTTTCACTAATATTTATTTTTTCACTACCTACTTTTTTCCAAAGTGTTTTAGTAATTAAAAACAAGAAATAGTTATTGAGTTTTTCATAATGACCAGACTTGCGAACATATGGCAAATGGGAAGATAAGCCATTTAACCAAGGGGTTGGATTAATTTGGTTAACATTGGTATTAAGTTCAGTTGTTCTACTTGTTAGTTTGTTATCTAATGCATCCATTTGTAAAAACAAATCTCTGATTACATTTGTTGCTAATGCAGCTGGTGCAAATTTTTGCATTAGTTCTTTGTAATCATTGAATGCTTTTTGCATCGCATCACTACCTTTATCTTTTTTTTGAAAATCAGTATTTATTAGTGCGTTTACTAATATTAATTCCTGATTTTTTTCAAACCAAGTTTTCAGTGTTTCATTTAATGGAAAACTAAAGGAATATAAACTGTTTTTAAAGGTGTTTGTTTTGGCTTGAAAAGCTCTTCACATTAAAAACTGCTTTTGCTTTTCAATGTCTCTTTCAGTTTTGCTACTACTATTTTTTGTCAGATAATAACTACCTCCATCAATTCCAATTACAGCAACTCCCTCTCCGTTTCTAACTAGTCTTAGCTTCAATTGGTTATTAGAGTTATGGCTTGTATTTTGAACAGTAGCATTTGCACAGTTTGCACTAACAGTGCTAGCACCATTATTTGTTTTGTTATTAGGAATAGCTGCATCCCAAACAAGATAACTGCTTGTTGTTGATGAACTAGCTTTAGTTGAAACCATCATATCACTTGTAGTTGTTTGGTGGATTTGCTTTGCTAGTTTTCCTAAATTGGAACTATCATTAGAACTTATAAAAGCATCTTTAATAAGACTCTTTAAATTGTTTTGTGAACTGTCTGTTGTATTGTGTTCACAGAACAATGTATTACTCTTAGTATGGTTTTCAATTTTAGTTGATTCAACAACAATGGTATTGTTAAGTTCTTTATTAAGCTCTTTAATTGTGGGAAGTTGATCTTTATCTTGATTTAAAAGATCTAGTTTTAAATTTAAACTAGCACCTAAATTAGCTGCTTCTAGTTGATCAACAATGTTTAGACTATCTACTATTTGCTTGGTTTGAGTGTTATCAGAAAGAGTTGTTGGAAAATCAATCAGGTTGCCTTGATTTTGATCTAAGATGTATTTTTCTAAACCTGTTTTTGCTGTATTTAATCCGTCTTTTCCCTCAATTAACTGCTCTCAGCGTTTGTTGCCAACATTCTGGTTCTGAGGAATTTCCCCATCTTTAGGAAAGAAAGGAAACGCATAAGAAGGGGTTAATTTATCTTTTAGTTTTTCAGTGTTGTAAACTTGACCCAAACCTTGTTGTGGTGCTGCATATTTAAAAGTAGCTTGACTGATTAAAGTAGGATCGGTATATTCAACCCAGAGATTAAAGATTTGTGCTTGTAACTTTGCAAACAATGCATCATTAGTTTCACTAAAACGTGGTTCTTGAAAACCAAAGTCCTTTCATTTTTCAGGTTGGGATAGTTCATCATAGATATAAGGACCAGTTGAGAGTTGTCCATTAGGTTTATAAACTAAGAAATCCTTAGCGAATAATTTAGCGGTAAAATCACTAATAAGTTGATTGAAAAGTTGTTGGGATTTTCAACTAGCCTCACTGCCACCACTTTTATCTAATAGATCTTGTTGAATAAATACTGCTCTGTTTCTGGGTGGTGATATTAACCCATTAACAGTATTTTTATATTGATCATCAACATTCTTTTTAGTGTCTTTTAAAAACTTGTTTATGGCTTTGTCTTCATTGTTTTCATACCAAGCCTCAAGTGCTTTTAAAAGTGGTCCTATAACAAAATGTTTGTTTGCTGAAGGAGTTTTTAAAGCAGTAATAAGTCCTTCTTTTAAACTACCCTTTTGACCATGAAAAAAATTAGTTGAGGTTGGTGAAAAAACTGTTTGCAAAGCACTGTTTGTTGGAGTTGAACAAGCTACAAGTATTGTGCTTACTGACAAACCACAAAGTAAAAACCACCAGAACTTTGATTTTTTTAAAAGTTTTTTCATGATCTTGGTTTTTTCTTATTAATTAAAAGGTTTTGAATCTGTTGTTTTTGGTATTTTTTGTAGTTAGGTTTAACCTTTTTATATTTAGCTTTGATAGCTTTAATTTTCTTAATTTGTAAAGTTGAAAGCGGTTTTTTGGTAGTTTTTTGGTTATTTTCAGTCAAAAACTTCAAATCACCATTACTAGTTAAACTAACGTTTTGAAATTTAATTCCTTTTGCTATTAGTTTATTTAATTTACTTTGATTTTGGTCATTTCAAAAAAGATATGATTTGCCCCAACTGTTAAGTCGTGCTACACGACCAGAACGGTGGATGTAAAAACTATCAATTTTAGGTAGATCTCAAGAAATCACCACACTGAAATAATTTAAATCTATCCCACGTGAAAATAGATCAGAAACAACTAGTAATTTCAATTTATTATTAGTTGCTTTTGTGAAATTATTTTTACGTTCTTGATAGGTTAAACTACCATAAATTGAACCAAAACTAATATTGTTATTTGAAAGCAATTGTGTTAATTGTTTTAAAGATTTTTGATTAGAACAGAAAACAATAATTTGCTGATTTTGGTGGTGTTTTAATAAGGCTAATAAACCAGAAAAACGGTTTTCAGTATTGAGATGAACAACAAAATGCTTAACTAAGGGGTGAATCCATTGCTTTGGATGGAGATTAATAACTTTAGTATTAATTACTTGTTTTTTAATTATTTGGAGTTGTTGATTGAATAAAGTAGCACTAAAAAAGGCAAACAAGGGCTTTGCTTTTTGAAAAAGATGAACACATTCAAGTCACTGCTGGATTGAACTTTGCTCTAAAAACATGTCAATTTCATCAAAAACAACGTATCTAACTTTGCTAGTAAAAAGGTATTTTTCGTTTAGTAAACTAACAATTAGTTGTGCTTTTGAACTAAATGAAGTGGCAAAAGTAAAGGTTTTAAAGTACTTTTTGATTTCAGTTAAGATGTTGATAATTTGTCATTGTAACTCTTTGGTTGGTACAAAAATAACTGCTTGAGGTTGATCTAAACTGGTGTTAATCTTATCTAATAGAGGTAAAAGATAAGCAAAGGTTTTGCCACTACCAGTTTCAGCAATACCAATGATATTTTGGAATGGTCATAATTTAAAAACTGCTTGCTGAATTTTGGTAAATTCAACAATGCGTTTCTTATCTAAAAACTGCCTAATAGAAGAGGAAAATTGCATCTTAAACTAGAATTTACGCACTCACTGTGAACTGATTGCTGAAAACAATCTGTTATCACCAACTCTAACTAAGCCATTTTTGGCATTTGCTATCAATGCATTAATAGCTTGGGTTTGATTATTAGGATTAAGCGCTTCAAGCGCAACTGTACTTAAAAATTCTTCAAGTGAAAGATCAAGTCTTTTTGAATTATTTGAATCTGTACTATTCAAACTATCACTACCAAGCATACTTACATCTTCAAGGTTAATCTGTTTTGCATAAACTGCATGATAGATCTGGTTATCTTGTAAAAAGTTATAACTGCTATAATTACTGGTTTTATTTGTACCTATATACCCTTCAAACCTACTAAACACATCATTAAAGTTACTATTATTAGTTTTACTTTGAGCTTGTTTTAATTGAACAGTTTGAGTATTGTTAAAGTTTGAATTAGCAAAATTCTTCAAATTAGTTTTTTGTTCTTGTAAGGATTTTGATCTATCAACACCAGTAACGTTAATATTAAGGTTACTTGTTAAATGGTTATAAAGTGCATCAAACTCAGCTTGGGTTGGGATGTTATCAATTGTTTCTATAAGTTTTGTTAGAGAACCAAAACTAAATAAAGCACCTTTGTAAATTGTTTCATTACTACCACTTGATTGAGTAGATTGTAATTGTGCTTGACCACCATTAGAACTTGATTTTTCAGATTGGGAAACAAAACTTGTAAAGAGGCGATTTCTAACTGCATCTGGTAAGTTGTTGGATGAAGTGTTGGTAACAATACCATGGAAACCAAACCTTCTTTGCTGGTTATTGGTTTGCTGTGCACTTTTTTGCATTAAAGTTAATGATGTACTGCTACTACCATTGTTCTTTGTATAGTAAAGACTTGAAATATCATTTGCAGCACTGTTAACTGTTGGGGTTTTGTTGTTGGTTCAATTATAAGCACTCGAACCAAATACATAGTTAGGATTTTGTTGTTCTTGCTTGGTAAAGTCTTGATTAGTTTGAGAAGCAACGTTGTTAGCCATTTTTACTAATACAGATTTAGAAGAACTTACACTCATTGCTTGACTAGCAGTATTATTTCTGTCTTTATCACTAGCTAAACTTCAAACCAAGAAACTATTTGTTGTTCTTGATAGTTTAGCTTGCAAGTTCTGTTTTAGGTTTTTAAGGTTATCTTTTAATAGCCATTGAACTGTATAAAGATAAGAATAGTAATTGATTAAGTCATTGCTATCCAAGCGTTCTTCTAAGTTTAAGCTGTTGTATAAACTATCTAAAACCCCATTAATTTTGGGTTTATTATCAGTTGAACTTTCATTAACACCATTACCAAAAATAGAAGGATTAGCTTGTTTTTGAAAAAGATCAAATAGGTAGGTATATTTAAAGATATTTCAGTTTTCATTATTGAGACTGTTTCAGTTTTTAAAAGTTAATTTATCTAAATCAATCGCTTGTTTAAACTGATCATCATTGACAAAATAATCTCGTCTAATGGTATTTTGTAACTCATCACTTGATAAAACAGCATTTAAAGCTAAATTAAGTGCTAGAGAAGTGTTTCTAGGATCAGATGAAAGTTTGGTATCAACAAGGATAATTTGTGAATATTGCGGTGAGGGAATTACCGAAACTTTTAGTTCATCAACATGCTTTTTAACTGCTGCTTCAACTTTCTTTTTATTCTCTTTTAACTTGTCAATTATCTTTTTTGAAAAAGGATCACTACTACTACTATTACTATTACTATTACTATTACTTGTATTTGAACTAACTTTATCAATGATGTCTTTATAGTAAATACCAAGTTCATTGTAGTGTCCTGTTTTACTATCTTGTTCATAAGGTAAAACAGCAGCTAAACCGTTCATCCATATTTGTTTATTTCGCTCATTATCAATGAAAGGTTGGTTACGTTCTGCTAGTTTTAAAGCAACTTTGTTTTCTGCAGTAGCAACTTCATTAAAAACAATTTGTTGATATTGACTTTCAACTAAATCCTTCAACTCGTTTTTAACAGTGATAATGCTATCACTAAACTTTTTAAACTGAGGGAAACTTAAAAAGTTACTTTCTTTGCTGTCTACTAATTTAAAAAGGGCATTAAATAAAAATAAAACTCTGTTCTTATCAAAGTGTTTTTTCACTTCATCAAACAACTTAAAGTCATAAGTAGTGTTTGTATCAATTAAGCCATATTTGGTTTGTAGTGCACGGAATAATAAGAATTGTTTTTGTTTATTTACATCACGTCCTGATTCAGAGAGATAATAACCACCACCATCAACACCCATTAGATGGATACCATCTTTACCACGTGCTAAGATTAAATCAGGTTGACTATTGTTATCTTTTTTAAGTTCTTTTAGATCAACGATTGCAGAATTATTACTACTATCACTGTTACTTGTACTAGTAGCATTACTGAACTTTTTTTGATATTCACCTTTAAAAACATCTGTTGATTTAGTAGTTTCATTTTCTTTAAAGATCTTAGCTAGATCTGTTTTATGAACAGTAGTTGTTTGTGAACTACTACTTGCACTTTTCATTGCAGCCTTAGCTGAATTATTTTCAACAAAGAAATTTTCAATGATTGATTTATCTTTTTCTAATTTAGTGTATTCAGTTTGTTCTGCACCTTTGGATTTTTTTTGTAAAGCTAAATAGCCCTGGATAAAAGCTGCTGAAAAGCTAGGATCAAAGGTATCAAACATATCACTAGCTTTTAACAACAATTTACCACCACTATCACTAGAAAACTTATTAGGGATGTCAATGGTTTTATTGTTAGTATTTACATAGCTTTGTAAATTACTAGCTAGGATATGAAAACCATTTGCTCCTTTTATGGAGTTATCTTGTTGGTTATATTTGTTAAATACTTGAAATTGATAGGAAGGGGTTGGAGATTTAATTAGATCAGGATTAAAATAGTTATCAAACCCATCATTAGGAGTTTCATTAGTAAATACTACTCTTGAAACTAAGTTAGGGTTTTCATTAGTAACGAACTGATCTAATAATTTATCTTGAATGGCAGCATAAACTGCATCATTATTGATCCTAAGTCTTTTATTTTTATCAACAAACTTAGCTTGTATTTTGATATTATTCCAATTACTTTGGTTTTCAATTAAACCTTTTAATGGAGTTGATAATACGCCAACACTTTTATCAACATATTCAACAAAGTTCTTTGTAAAAAGCTTATTGATAAAATCATCAACAATTTTGTTGTTAACATCACGGAGCTTTCAGTTGGCTTGATTTCCACCAGTGTTATCTAAAATATCAGTTTGTAACCTAACTAAATAATCACCCCGATACTGATTTCTTAAGTTTTGTTCTTGATTAACAAAACTATTGTCTGTATCAGTATTAAAAGTTCTTAAATTTCTCTTAATGTCATCATCAACATTTTCTTCATAAAAATTGCGCAATACTGGCGCTAGGCGCATCGTTAAATATTGCGTTAGTCCTTCTCTATTTTCAAGTGCTTTATAAAGTGTGGCATTAATACTACCATCATTTTTATCAGTAAAAGCACTACTGGGTCTAAAGAGATTTTCAATGGTTCTGGAGTTTGGTTGTGCACAGGCAACAGCAACAATTCCCAAACTTACAGCTATGCTTGAAAGCAGCAATGGTCATTTTACTCGGTGTAAAAAACGTTTCATTGCACAGTCAAAAATTTCAATAATTTTAAAATTTATATTTTTATTATTACCACATGCGACTTGAAATAGAAAACGGGCTTGAATTTGTCAATGATCCTGTGGTAAATGAACTTGGCAAGATCTGTTTTTTTCATCCTTTTACAGGTAATTTAACAAACAAACTTAGTTTCAGAAGTCATTTCAATAGATACAGTTTTTATGCCATTAACTACCCAGGGCATGGTAATAGTGTTATTAACAATCCAAAGCAACTTGAATTTAGTTACTGATTGGAAATTACAAAACAGTTTTTTGATAAACATAACTTAAAGGATGTAATCTTATTTGGTCATTCTATTGGCGGTGGTTTAGCAGTTGCTTTAACTAACTATTTAAGCAGTGATCAATATAAAGCAGTCTTGTTAGAAGCACCATTAAATCCTGCTATTGTTGAAACTCCTTTAAATATTGTTCAAAATCTCATTCCTGATCCTGATAGTGATTTTGCTGTTATTCAAAAGTGTCTTGTTTACAATATTGAGAAAAAACTTGGGGCAAACTTTAAAGAATATTGTGAAAGAGAAAAGCAAAAATCAATTCACCAAAACCAACGCCTTAAAGTGATGTTAGAACCTTCTACACTCAAACAAAACATAGTTTTAATCAATGCAGCTTTTTTAAAGTTAAATTGTCCTGCTTTGTGAATCCATGGTAAGCAGGATGGAATTATTAAGTACCTTCCATCCAAGGCTTATTATGAATCATTAAACAATAAGCAAATTCAATTTAAAGCCATAAAAGCAGCGGCTCATACCCCTTACTTTGAACAACCACAAAAGTTTTTAAGTTTAGTTAATGATTTCTTTCAATTAATAAGTTAAAGCAGTTTATTTAACTAAACGCTTGTATCACTCAACAACGTAAGATTCATTTATTCCAGCAGGTAGTTCGCTACGCTCTGGGAATCTTATATAAGTACCTTCAAATGCCTTTTTGTTGGTTTCCACAAATGATGAGATAACACTGTTTTCAATAAAATTTTTAACTAATGGGGATTTAGTTATTCTTGCTTTTAAACGGACTTTATCCCCGGGATTAATGATGATTGAAGGGGTGTCAACAGTTTGATCATTTAAAATCACATGACCGTGGTTTACCATTTGTCTTGCACTCTTTCTTGTTGGTGCAAAACCCATTCTGTAAACTATGTTATCCAAACGTGATTCTAAAACTCTAAATAAATTAACTGTTAAGTTACCTTTTTGTTTTAAAACAAAGCGAAATAACCTACGAAATTGTTTATCAGTAATACCATACATGTATTGCATCCTTTGTTTTTCTTGGAGTTGTTGGGCATAACCTGATAAAGTTGAGGAACGAAACCTATTTCCATGTTGACCGGGAATAGATTTACGTTTTTTTCCTCTGGAAAACTCTTTGTTGTTTTCAAGTAAAGAAAAACCTAAACGTCTTGATCGTTTGAAAATACTACCAGTATATTTCATGTGTTTTCGATTTTAAATTATCTTAATAATCAAGGCTAAAATCACCACTGCTTTCAAATAAGTCTTCATCTAATAATGAACTGAAATTGGAAGTACGATCAGGAACTAGTGTTTCTATATCCAAATCATTAACACTAAAAGTACTCATTGAATCCATTACTTTGGCATCAATGTCAACAAAATCATTGTTAAAACCTTCAAATTCAAAATCGATGTTACGATCCATGTTTTCATAGATAAAATCATCTAACTGTTCATTGGAGATGTTATCAAAAGTGTTGGAAAAATCTATTAAATCATCCATTAACTGATTAGGTTCTACTAACCCATTATCTGTTACACTATCAAAATTGCTTAAAAGTAAAGGATCTTTTTCTTTCTTTGCAAAGCGGAGCGTATTATCAAGCTTATTAATGTTGTTCATAATAGTTTCACAACAAGAGTTATAAACAGTTTTAAGTTGTTTTAAACTACCTTGAATGCTTTCTATCTTCTTTTCATTTTCAGTGATAGCACGTTGAACATGACGCATAATTTCTCCAGATTTTTGCAGTTGTTCATAACTATTACTTGGTTTGTAAGCAATAGCAAAATCACTAGCTAATGATCTGATCATTTCTTCTTTTTGAAGTGTTGTTAGATTCTTAGCGTTATTAAATTGAACGTTAAGGTTCTCATTTTGATCAATTAAACGGGAAAGTTCACTTTGTAATTCCTTTTTAATTATTAAGTAACGTTCACTAATTTGGTTCTTATAGTTGGTAACAAAATCATTGAGATGGATAAATGATTGTTTAACAGACATCTGATAATCATTCATTTGAAAGATTGCATCAAAGTTAATTGTAGGGTTTAATTCACCACGTTTCAATTCACTAATAATCTTGTAAAGATCTGATTTTTTATTGAAATTATCAAGTTCACTTTTATCTTCAACCGATTGCTTATTTTGAGTAATTGAGTGTTCTTCAACAGGTTCTTTTGCTTTGACAAAAACTTCAATTTTAGGTTGTTGGTCTTCGCTATTAACAGTATTGATAGTTAGTTGATCATCTTTTTTATCGAGTCTTACTGATTCACTAGTAACAATTGATTGATTATTTAAGCTATTAATTTGTTGCAATTGGACAGATAATGTTTTTGCAAGTGTTTTGGAATCTTCAGTTATGGAATTAATGGTAATTTGTTCTTGCTGACTTTCTTTATTATCTAAAGCTAAAGTTTCAACTGGTTTATATTCAATACGGTTGGATACAAAACTAATAGGTAAAGTTGTAGGGATTTGGGGTGAACTTTTTTTGGTAGTGATAGTTATCTTATTATCAGACTGAATATCAAATTTCTGTAAATTATTAGTGTAGTTACTAGTTTCAACTACATCTTGTTTATTGAAACTACTTTCTAAAACTGGCTCCTTAGTTATTTCAGGTTTGAATTGATCGTTAACTTCGCTATTTACTTGATCAAACTGCTCAACTGTTGGTTGAGTTTCAAATGAAGAAGAAAAAGTTGTTTGGTCTTGTTGATCTGTATCAATTGGTAATTGGTAACTATCAACTACTTGGGCTTGTTTACTTTCAAAATCAGTAGTTACTTTTTTTTCAGCAGTTGGTTGTATTTCCTCATTATTATCAAGATTAGCTTGAACTTGATCAAATCCAACTTGATAATTATTTTGTTCTTCAGCAACTGGTTTGAGCTCTTCATGGTGATCAAAGTTGTACTGAACATGATCATCTAAAGAAGGTTGGTCAAGGGTTGGTTGAGGTAAATCACTGTCAAAGCTGTAGCTATCAGTTGTTGATTCAGGTTGTTTTGCAAAATGATCATCTGAAGAAGGCTGGTCAACAACTGATTCAGGTTGTTTTACTTCTGGTGGTAGGTCGTTATCAAAACCATAGTTTTCATCAGTTGGTTGTTTTGCAAAATAATCATCTGAAGAAGGCTGGTCAAGAGTTGGTTTTAGCTCTTCATTTTGATAAATGTCGTATTGAACTTGATCAACTGGTTCAGATTGACTTTCAAAGTGTGCATCAGGTGCAGTTTCACTATTAAACTTAACAGGTTCAGACTCTAACGAATTATTGCTGTTTGATTGAGATAAATCACTCCCAAATTTATAGTCATGAATTGGTTCAGATTTACTTTCATAATGCACATCATCAGATGCAGTTTCACTATTACTATTAAGTTGAATGAATTCAGAATTTAATGAATCATTGTTAGTTGCATCTTTGTTGTTTTCAGAGTAAAGATTAGATTCAGAATTTAAGTCACTAGTTTCAACTACATCTTGTTTATTGAAACTACTTTCTAAAACTGGCTCCTTAGTTATTTCAGGTTTGAATTGATCGTTAACTTCGCTATTTACTTGATCAAACTGCTCAACTGTTGGTTGAGTTTCAAATGAAGAAGAAAAAGTTGTTTGGTCTTGTTGATCTGTATCAATTGGTAATTGGTAACTATCAACTACTTGGGCTTGTTTACTTTCAAAATCAGTAGTTACTTTTTTTTCAGCAGTTGGTTGTATTTCCTCATTATTATCAAGATTAGCTTGAACTTGATCAAATCCAACTTGATAATTATTTTGTTCTTCAGCAACTGGTTTGAGCTCTTCATGGTGATCAAAGTTGTACTGAACATGATCATCTAAAGAAGGTTGGTCAAGGGTTGGTTGAGGTAAATCACTGTCAAAGCTGTAGCTATCAGTTGTTGATTCAGGTTGTTTTGCAAAATGATCATCTGAAGAAGGTTGGTCAACAACTGATTCAGGTTGTTTTACTTCTGGTGGTAGGTCGTTATCAAAACCATAGTTTTCATCAGTTGGTTGTTTTGCAAAATAATCATCTGAAGAAGGCTGGTCAACAACTGATTCAGGTTGTTTTACTTCTGGTGGTAGGTCGTTATCAAAACCATAGTTATCTTCAACTGATTCAGGTTGTTTTACTTCTGGTGGTAAATCGTTATCAAAACCATAGTTATCTTCAACTGATTCAGGTTGTTTTACTTCTGGTGGTAAATCACTGTCAAAACCATAGTTTTCATCAGTTGGTTGAGATTCTTTAGTGGAGATCCACTGGTCATTTTCAAAGTAACCAGATCAAACCCACTCACCATTTTCATCATAAGAACCATAGTCAGGGTTACCAACATACTTTAAATAATCATTCTCTTCTTGCTTTTCATCAAAAACAAACGGATTCCCATTTTCATCAAAGAGTTGGGAGATATCATATTCAGTATCTCCATAAGGATCATAGTAAACCCCTGTTTCTTTGTTAAAAGCTATATAATAGCCATTACCATCAGCATCATATGCTACCTTAATATTAGAGTCTTCTTCAACAGTGGATTTACCATCATAAAGATCACCAAAAATGTTTTCAGGTTCGGTTTGGGTATAGTTTTTTTCTTCAAAAACCGATTGCTTGTTTTTAGCCATCAAGAAAAGTTATTTATGCCAGTTTAGTCAAGCTTGATTATAAAACTTATCGCTTCGCTCTAACTCTTCCATGAAAGCGTAAATAGTAATAAATAATTTTTTAAATTATTTTCCACAATCCCTATTAAAGGGTAAAACAAAGCTACATAGATAGGTAATAATAACGCTTGCACCAATACTCTTGGTAAAAGAAAGAAATAAACCCCATACAAAACAAAGTTATTACTTGGTCTGCCATTATTAATAAAAGTAATAAACTCAATGGCAGCAATTGTCCCCAAAAGGATAGAAACAACTACTGACATTAAAACCATTAGAACCAAGCTATAAATAGTTAAAAGTGCTTGTTCTTTATTTCTCTTTTTTACATAAACAAACCCTAAAAATCCTTCTGTTACTAAAAAGAAAATAACAAAGCTAACTAAAGTTATGATTTGTAAAACAACAACATTAACACTAAGATCAGCAGTTTGAAAATTTTTTGTTCCAATTTGCTGAAACTCATTATTAGTTAGATAAAGTAAAAGCAGTGTTACACTAACAACAAAAAAAGTTACTAATAGTGTTTGTAACACCCAAAAATCAACCTTGTAATTACTGGCATTTTTTCTAACTAGATAAATCCCTTTAACTATGCCAGCAAGAAAAGCAAAGAGTGGCTTTTGCAATGCAAACATCCAAAACCACACATAACCCTTTGTTAACCAATCAATAGTATCAGATAAAAATCCAAAAATAAAACCCCAAATAGGACCAAAGATCCATCCGAACAATGCAAAGGGAATCCTTAGAAAACTAATGCTTAATACATTAGTAACACTAATTGAAAAGATAGAAAAGATAAAGGTTAGTGCTAATAAAACACTAGCTCAAACTAACAGTTGTAAACTCTTTAAAAGTTTCAACGGATAGTAAGGAAACAAAAAGTTTCAAAACAGTTTTTTAGATTGAAGCTTGAATGTGAATTTTAACCAAAAATAGTAGGTGGGGGGATATCTAAAGTAGTTTTACTTTCATCACTAGTAATTGGTTTTGCCAATTCCTCTTGAAAGTACTTATTAAATATCTCATTAGTTTGTTCTAAGTTTAATTTAGGACTTAATGAAAAAGCAATTACACTAATGATGCTAAAAGGAACAATGCTAAAAACAAACAATCAGGCGATTCCTATCAGATAACTTGCAAATAATGGTGAAACACTAATTAGTTGGTTTTCACTAAATAAGCGGATGATTAAAGCTGCATTTCAAAAGCCACTTCTTACTATATTACCAGGGATACAATTTAAAAAGACAAAGATAAAGATAGTCCAAAAGATCCATTTTATTCTTACCTTATAACGGTAGTTATCATTAATTTTGGGAAACTCTTTGACCCCAAATTCACTACGTAAATACCGCATTGCTCTATTGGTTTGACCTCTAGTTGCAAAGCTATAGGGTTTACCCTGAAAAGCAAAGAAAAAGAAGCACCACCAAAAGCCAGGAAGATCATGGTAATCAAGTTAGATGAACTTAAAGCAAAAAAGCGGTTTAGATTTGTTGTATCTGCATTACTTTGCTGAACAGTATTTTGGTCATTATTAACATTGAAAAGTGGACTGTTCTTATCAACAAAGTGGGTAAAAAAGACATTGACCATCACAACGTATGCTATCGAAATGAAAAAGATGGCAATCATAGAGATGAAAATCGCTAGTTGTGCTCATTTAATCCACTGACTCTTAAACTGAAACTCAAAGATCTTACCAACTAGTTTTTCAGAGGTAAACCTACCAAAGGCACGATCTCTGGCACGAACTTGGAGGGTTTGTTGCAAAATATTATTCTCAAGTACTGAGTTTAGCTGTTGAGAATTGTTTATAGTTTGAAAACTTGGGTTATCTTTAACTGTTTTATTGTTTTCTTGTAACTCACTAACGGGTTTAACTTGAGTTTCAGCAAGTTTGGTAAAGCTATTCAACTCTTTATTAAACAAGTTTTTAACAGCAACATTAAGATAATTAGCTAAATCTAACGCATCAGTTAGATCAAACTTAGCAGCTTTAAGTTCTTTTTCACTTAATTGGTTTTGCCCTAATTGGATAAACTTATCATGAAAACTACCAAAAAGAAAGTGGTCTGGTTTTTTGTTTTAACTCTTTTTTGTTTTTCCTCTAAAAAATAAAGTAATTTGAGTTGTGCCAAAATTGTTTTGGCAAAATCACTATCAGTTTTTATCCTTGTTCTTAAAGTTTCGTTATTAAAAACAATGTCAAGATTATTTTGTACCTTAGTTAGAATTTCACTAAACGTCATTTTGATAAGTAATTTTAGAGAGGTTAGTTAGTAAAAAAGCTTAAAGCAAAGTTTTTTAACAACGGAATGGCAAAGCCATCATTGTGCTTTAATTTAATATCCAATTTTTGAATTGCATTAATTATATTTACTAAAACTTTGCTAGTAAAGTTATGGTACTGACTTTGAATAGCAATTAAGCGAAACTGATTAACATTTAGATTAATGTTAGTTAGTTGCTTTGGATTACAACTTTTAACCATCAATGCTAATAACAACTCCCCACTAAAAAATTCTAAAAATTGGGTGGCAAAATTTGGTTTTGTACTTGCCAATTCATCAATATATTTAAATGCCTTTACAATTTGTGAATTTGTTAGAGCTTTGGTTAATTTGTAAATTTGAACAGGTTGATAATCACAAATAACTGTTTCAACTAATTTGTTATCTTTGATTTCATTTTTACCTAACAAGCTAAGTTTGTTAATTTCTTGGTAAATTACCCCCATATTTAAAGGTAAAGCATTCGCTAATCAATCAATTATTTCCAAATCAAGTTTTAAGTTAAGTTCTCTACAAACTTCACCAATTGCTTTATGCATTGATTTTCAATCCAATTTATCACAAAAAACAGTTGTGATTGATTTAATGGTTTTAATCCCACTAAAAGGATTGTCAGTATAAACAGTTAAAACTACATCAGTTGTTTTTAGTTTTTCTAAACACAAGTTTTCTTGTCTAGTTAAGCTTGTTTTTTCCAAAAAACTGCAATTATTGACAATAAACTTTTCATTATTAGAACCAAATAATGGTTGTGAAAAGAGATCATAAAGTTGCTTAAGATCTTTAAACCAAATAGTTTTATATGGAGAATTGCTTTCGATTTGACTTAATTTTTGGTGAATTAGCCCAATATCTTGACCATAAATTATTGTCATAATAAGCTACTGTAAAAATAGTATATAAAAGTCCCAATAAGCCATAATAAACAAAAAAGAAAATCAGATTAGGTTTAGGTATTTGTAGAAAAACTGTTACTTTTAATGATCATTCACTTAGCATTTTAAGTGGGAGATAAATACCAAAACTAGCTTGACCAAAAACACCAATAAAGGGTAAAATAATTCAACTAACACAAAAATAAAATAAGGCAATTGGGCTAAACAAAAGGTTAAACAAGACACTAAAAGCATTTAGTCTTGAATTTAAATACAAGCTTAAGGGGCTGATAACAATCAAGATTAAACTAGAACTGACAAGCGGTTTTAAAGCTTTTAATAACTTTAGTTTGTTGACAAATAACAATACAAAACAAGCTAGAAAAGAAAAATTAAAACCAAAGTTATTTAAAGCATGGTTACTAATAAGAATGATTAAAAGTGCAGTTAAACTTAAGTTATCTTCTGGTAACTGTTTTTTAAAAACCTGTTTTAACAGTGTTGAGATAAAAACTCTTAGTGCTGAAAAAGCAAATCCTACTAAAAACAGGTAAATCAATAAAACAGCAAAACCACTAAGTTTGTTTAAGTACCATCTTTTTCATAAAAACCGTTCCATTAAATTAAACAAGAAACTTAAGTGAAAACCACTGATAACAAACAAATGGACAATGTTTAGTTTTAAAGCATTTTGGTACAAATTGTTTTTAGTGGTTTCATTAATTAAAAATAGTTTTAAATACTGATTTAACTCACCTTTAGTGGTCTGATCAATTCAATTAATGAAAGGGGTACGAATAAAGTTATTTTTAACAATTTGATTTATCTTGACTGCTGGAACAAAATAACAAAAAGCAATAAAAAAACCAACAATTGTTAAGTTCAATAAACAAAACTTTCAGTCATACCAAAGACTAAAAAGCGTTATCAAAATGATCCAAATTGACAGTGCAACAAACTGTTCTTGTTGTACTAAAAAGAAGCCTGGGATAAAACTGAGTAAAACAAAAAATAAAACAGCTTTGTTTGCATGCTGTTTTATTAATAGATTTATTTTGAACTTTTTAACAAATTAAAAGTCCTTAGATCAATATGATCTTGATGGATAACGTCTAAGTCCATCATAGCTAGAACTAAATGGTAAACTAGTTCTTCTAAAACTTGATAGTCTTGTTGGTGAATGGAATGATGAACGGTATGAACTGATATTAGTTAATCTTCATGGTCTTCTTGTTAAGGGATAATAACTACTTGGTGTTCTGCTAAAACGATAGTTAGAAATAGCATTATTTTGGAAATTAGTTGATCTTAATGGTGTGTAATTGTTGACTCTAGATTTTGGTGTGTAGTCAAAATCAAGAGAGAAACGATCACTAGTTAAACTTTGATTGGTATCGCTGTAAAGTGGATCACTGTATTTAGTTGCAACATACTCATTTTCAGGATAAAAACGGCTGAAATTGCTACTTTTTATAGTGTTATCACTCTTTAGAGCAATATCACTCATTATCCTTTGGTGGTTTAAAGCATTAATTTTCTCTAGAAGAGAAGCGTGGGAAGAGACACTAACAGTGATATCTGAATCAGTTGTGCGTTCAACTTGTCTAGGTTGAAAACCAGTGTCTAATTTTGATTGAAGTAAAGCTGCTGATCTTGGTACAACTTTAATTTGTAAACCTGAAGTTGGCAATGGTTGTTGGGGTTGATATTCAGTTTTTACTTCTGGTTTTCAGTGGTGAACTTCAACTTGAGGAGTTGATTCTACTTGAGGGATGTATTTTGTTTCAGGTTTTGGTTCAATTCTTGGTTCAACCATTTGAGCTTTCGGTTGAACTTCAACTTGAGGAATATGTTTTATTACTTCTGGTTTTGATTCAATTCTTGGTTCAATCCTTTGAGGTTTTAAATCAATAACAGGTTTTGGTTGAACTTCAACATGTGGTACATGTTTTACTTCAGGTTGTTTAACTTCTGGTACTGAATCAACAACAGGTTTCACTTCTGGTTTTGGTTGAATTTCAACATGTGATACATGTTTCACTTCTGGTTTTGGTTCAATTTTGGGTTCAACTATTTGAGGTTTCGGTTGAACTTCAACTTGAGGAATATGTTTTACTTCAGGTTGAACTAACTTTGCTGGTTTATTAACTAGTGGTGGTTTTAAAGAAGGGGAATTATTAAAACTATGGTTTTGAGATCCACTATCAACTTGAACGGGTTGTTGGTGGACTAAATCCTGATGGAGCTGTTGAGTAGGTGGCACTTCTTTTTCAACAAACTGGTCATCACCCAAGTTTTCTGTAGGTTGTTGGGGTTGATTTAAACTGTCATTTAATTCAAAGACATCAGGTTCACTTTCAGTTTCTAGAGTAGTGTGTTGAGCTGCTTTTGGTTGGTCATCAACTTCTATCTTAGGAAGACCTGCATCAATACTAGGATAATCTTGTTGGGTTGGTTGTTCTTGAGAAGTTTGAGTACTGCTTAAATAGTTAATTAAAGGGACCTTTTTCTCATCAGTATAGCTAGAAACATCCAAAACATTACCATTTTGGTCATAGAAAGTGTTGTTATCCTGATCATATTGAACAGTTACCAAGTTGTTCTTATCATCATAAGCATTTAAGCTAACAATGTTATTTGTAAAGCTAATAGGGTTATCTGAAGTTAATTCAAACAAAGGATAGTTACTAGCTAACTGCTTTCTTTTTTCTAAAGAGAAGATATTACCTGATTCAGGATCTTGGTATAGAGCATGAACCTTATTTTTATGATCACGGGCATAAACAACACTAACATCAGGATATCTTTTGTTAATCTTTTTTAAAAGCTTGTTATAGGCTTTGTTTATTTTAGCTTTCTGTTTATCGTTCATAAAAAATTACTAAATTAGGGTTTTAAACCGCCTTTTGGCATCAATTGTGATGGGTTAGAACGTCGTTGCATATTTGGATTAAACCCAGGGCGTTGTTGCATTTGGGGATTAAAGTTATTTGGTTGTGCAAATTGGTTAGGATGGGGTTGGTTAAAACCAGGGCGTTGCATGTTTGGGTTCATCGTTGGGTTGAAATTATTGTGTGGTTGGAACTGTTGGTTTGGTTGGTTAAAACCAGGGCGTTGTTGCATGTTTGGATTAAACCCAGGGCGCTGCATGTTTGGGTTCATCCTTGGGTTGAAATTATTGTGTGGTTGGAACTGTTGGTTTGGTTGGTTAAAACCAGGGCGTTGTTGCATGTTTGGATTAAATGCAGGTCTTAAAAGCGGAACACGCTGTTGAAACTGTGGTTGAAGGGGTTGTTGAACTTGGGGTTGGGAAGGCTGAGCTTGGGATTGTTGAGGATCAATTTCAACTGTTTGTTGTTCTTGTTGATCTGAGATTCTTTGTAATTGCTCAGCAATCTGTTCTTGGCGTTCCTTCTCTTCAAGTAAACGTTTTTCTTTGCGCTTTACAATAGGTAAACCTATCGCAAGTCCAAGGATAATAGCTAAAGCACTAAAACCAAATGAACCTGCTACTGTTGGGATAAATCAGGGCTGTTCAACAATTGATCTGCCCTGAATTACAGTTGTATTATCTTCTGTTTGATGAAAAACAACCCCTAGTTCACTACCATTAGTTTTCACAACAAGTGCAAATGCTAGTAACGATAAACTAACAATTAGGATTGTTGTAAAAAGTAAAGCAAGAACTATAAAAAGTTTTTTGTATCTCAAAAATCCATTTAACTCCATCTTTATCAAAACTTAATTAGTTCCTTTTTCTTCTTGATCTTGAATCATCATAATACTCTTCTTGTTCTTGCTTTTTCATGTTCCTTTTTCTTCTTTTAATTGAACGAGTGATTTTATTGAGAAAGAAGTTAACAATTAAAGTACCTATTAAACCACTAACAAATGCCATTCCTAGGGGAATTACATACTTAGTTAGATAAGGTGTAAACTGTTCAACAAATCCAGGTAACTTAGCATTTTTCAAAAAATCTACATATCCTGGGATAGCATAAAATGCACTTTTTTCAGTTGGAAAACGGTTATCCAAACCATTACTTGAACCTTCTAAAAGTTGTAATAAGCTACTTGTTTGAGCAGTTGCAGTTGCACCATTGTTTGAAGAGATGTTATCCAACGCTTCTTTTGAAAAGAACCTGTTGAAAACATCCATTGGTTGTGCTAACTCAAATGGGTTTAGAGAACCAAAGTAAAAGATAGCTAAGAAAAGATAAGCAAAGCCTACCAATACCAATAAAAGAAAGCAAAGCTTAAAGAGAAATCGAAACAAGCGCATTTGTATTAGTCAAAAATAAAACTACTCTGTGAATTTTAACTTTATTTAGTTAAGCAAATGGTTTATGTAATTAATTAAATTAACTATAGAGCTTTAGAAAGCTCTAAACTCTAGAAAAATTTTTAGTAATTAGGATGATTCTTAGGATGAAATAGACCAAATTCATGAAGCTGCTAATTAATCAAGCCCCAAAGAATAAAGCAGATCTTCAGCTCTGTCTTCTTGCCATTTCATAATCTTCACTTTCATAGTATTTAATCAAATCTTGTTCATTACGGATCTGTAAACTCACAAAATAAACTGAAATTAATGAAAAGATTCCCCCTACTATTAAAGATAAGCCAGGGATAATTCTATCAAGTAGGTTTGTTGTAGAAGCAAAGACTGTAAAGTTCAAAATAAAGCTCAATAAGCTCGCTACAATACTAATTACAAAAGCCCATAATAAGATGTGAAACAAAGTTTGGGTTTGTTGATATCGCCTTTTTTGACTTGGTAGCGCACTTAATCCTCACACTGAACCAAACACCAAACTAGCACCTAAAAAAGCATAAACAATGTCATTAGCTTCAAAGTTAACTTGTTTTGAAAATGTTGTTGCTAAAGTTAATAAGATCCCAAGGGTTATCCCATAACTAATCACATCAGTAATGATTAAGGCCCAAAACCATGTCAAACTAGTGTTGGGATTCCTTAATTTAAAAGCAGTAACAAAATAAAGAATTAAAGAGACAAAACTAGTAATAACTGCAATAGTACTAAGTGTTCTAAAATCACTAGCTAAATCAATTAGTGTGCTTCTTGAAAGCAATTGTTGTACTGTTAATGCAACAAGAAAGATAAAAAAGATCCCAAAAGCAGCTACCATAAAAGTTAAACGGATTACAGAAAGCTGTTTTGTTTGTACAAACTGCTTTGCTGTTAAACCGTTTTGATCAATATAGCCTTTTGTTGAATTAATCATGACAATTTGACTTGTTAAATTTTAATTTGTCAATATAAAAAAACTAATAATTGCAAAATATATAGATAAGGATACTTACCCAAGTGGCTGAAGGGGTAGGCTTGGAAAGCTTATAGATGGGTAAAACCATGCGAGGGTTCGAATCCCTCAGTATCCGCCAGGGAGATTTACCCAAGTGGCTGAAGGGGGCGCTCTCGAAAAGCGTTAGGTGGTTATCCACGCGTGGGTTCAAATCCCACAATCTCCGCCAAATTCTTAACTGTATAAACAAAAAACACACCATTTTGGTGTGTTTTTTATGTTCATAAGTTAGTTGAATTTACTCATCAATGTAACCTGAATCACGCATTGTAGCAATCCCTGAAGGAGCATTACGGAGTGAAAATCATGGTTTTAAAAGCGAAGGAATAGTTTCATTTAACCCAGGAGCGGAGACTGGTAGAACAGGAAAGTAACTATCTACTACAGTAATTAAGAACTTAGCATTATCAGTGTCCTGTTCAGCTCAAATGCTGTTAAAGCGATTAAGTTGAGCAGCGTTGTCATAAAGATCTTTCAATTCATAACCAATGTGGATATCTGATACATTTGTTGGTTTGTTGTCATCTATCTTGCTTCTTTTTGATAGATATTTATCAGGATCTTTAATTGCTTCTTTAACTTTATCAGCTTTCAATACCTTATTATCAACTAAAAATTTCAGTAAGCTTAATGCTTTTTTAGTGAATGCTAATGAAGCGGATGTACCGTTATTACCATTAGTACCAAAGTTCTTAATGACATAGGGATCATCTTTAAAAAGATCAGCACTTGTTATGCAACTATACCAGTCTTTTCCGTTAGTTGTTGTGTTATTTTGAAGTTTATTTTCAATTTGATTAAAGACATTGTTTTGGGATGCAGCTAACAATAAAGTTCTGACAATATGACTACCTGAATTGTAAGTAATTACTTCACCAATACCCTCACTGTTAAGCTGTGTAGCAGCATCAAGTCAGGTACCAACCCCATCATAATCAGGACTCCAATAAGAAAAACCAAGTACACTTGATAAACCTATGTATCATTCGTTTCTTGTTGGACCTTGTGCATCTCCTAAGATAATCTCAGGGATAATTAAATTTTCACCATTATCATTACCAAGTTCAGTTAAAGCTTGTTTTAAAGCGTTATAGTAATCAACTTGGTTAGAGGAGAGTGATGCATTTCCTAGTTTAATGTTGAAGTGTAATGGATTGTTACCAGTAAACCCAACTTTTTTTAATGCACTGATAAAACTTTGCTTGGTAACACTTACTTGTTTTGAAGAAACTTGACTAGTTTGAGTATTGTTACTAGTAGAAGCTGTTCTTACTTGAGCACCATTAGTTTTTGTAGAGCTATAGTAACTTAACTTAACAGTTGCAGTATCAAGAACATTCTTATCCTTTTGAAAAGTTCCAGTTTGTTCAGGGATTAAACCAAGTCTTTGGTTGTTGTTAATCTTGTAATAATAATCATTCTCAATTCCATCTGTATTTGTACCACTACTAGTTCCATTTGCTGGTTTTTCTTTAAAGATCCCAAAAGGAACAGTAGAATAGTTAACATCTCCTGAGTTGGGCAGATCAATGATTGCTAAGTTCTTTCAGTTGATCAAACCATTAATTCCAGCACGGATAGTCAACGCTTCTTCAGATTCACTGTTATATAAGAACTTAGCTTCTGTCTCAGTGATGTTTGCATTTAGTTGAACAGTACTGTCATTAGCAACATAAGGTTTACCTGAATAAGTTACTTGAGCACTTTGCGCAGTTTTTTGGATTTTTAATCACCTCAGATCCCCTGTACCATTAAACCTTGATCTTGCATCTTGTTGTTGGGAATAGGGAACAGCTGAAGCTGATAAGCCCCCAGCGATATAACTAGAATAGAACACTTCAGGAGTAGCTCCTGGTTGGAAATAACTAACTATAGCAGGGATAGGTTTTTCGTTTTCTTGTCTTGTTTTAGGAAGATTAGGGGTGATAATTGCATCATAAATCTGGTTGCGTTTAAAGACAATTTGTGCTTGGTTAAAACTCTCAACATAGTAAGGGCCAACTGATCAAGTGTCTTTTCAAGCATTAACACCCCCTCCATAGATACCATCAAAGTTAGTATTTGCTTGATCTAAAATCTTTCTGTTGTTATTTTGGTTATATTTAAGCGGTGAATCTTTACCTAGTTTAAGGGCTTTTACCTTAGGATGGGTGTGAGGAATTGGAAAGAAGAATTCCTTTGACATCATTGATAGAAAGAAAGGAAAAGGAGAAGTTAGATAAACATTAAACTTGTTATCATCTACACTTCTATAGTTTTCAACATCATAATTTTCATCAGTGATCTCAATACCTTTACCATTGTTACCACTAGAACCGTTGGTTTTATCCATACCAACGGTTTTTTCAACATCAAGACCCATTAGGTCAATAAAATAACCATTCCGGTTAAAACCTAAGTTAGAAGAGAGAATATAGGTCTCAAAACCCCTTTCAAAGTCTTTAGAGGAGAGTTTAACTGGTTGGTTGTTTTGTTTGACTTCTCTACCAGCATTATCAACCCAGTTCAAACTAGTGTTAATGGTAAATTCATATCTGGTTGCTTCTTGGGTTATCTTGTGATAGTTAGGATCTTCAGTAATAGACTTTGAACTATCACGATAAATTGTGCCTATTGCATCCCACTCATTGAACTTAGTGTAATCATTCTTACTAGATCCATTAGAAGAACCTGTTGAACCATTTTTTTGTGCTCTACCTTTAAGACCACTGTGTGTTGTCTTTTTATAGCCAGTAACTGATAAAGCAAGTTCTAACACTAATTTTTCTTTAATTTGGTCAGTAACATTAACCCCATCATCACGGTTTGTACCAGTAGCAACATAGTTAGTTAACCCTGCATAAGCATTACCAAAAAAAGATGAAGTGGGGGAAACGTTAAACATGCTCAATGGAACAGAGTTGTTCTTTAACAAATCTGCTGAAGAAGAGGTGAAAAACACCCCTTTGTTTAACTGTGAGGATGCACAAGCACTTAGGATTAGTGCTGCACTAACTGTCAGTGTAGAACCGAGCAACAAGTATCTTTTTTTTAATTTCATTGTGTTCTTTCATAAATAAATCGGTTAATTGATTGCTGATTAGTAAAAAAGAGTTAAAAAACAGCAATTTACCCTTAGTTAAAAACTAAAAGTAGTTTTATTCTAGTTTGCAATCACATTAGATTGCAAAATCAAAAACTAATGTTTTTATCAATTGCTCACTTTTTAAACAAGAAAATGCTTAATACAAAGTTAGCAAAACTAAAAACATGGTGCACCCGAAGGGACTTGAACCCCCACTTCTAGATGAAACTAGATCCTAAGTCTAGCGCGTCTACCATTCCGCCACGAGTGCATTATTGGTGCATCTTGAGGGGATCGAACCCACGACCCAATGATTAAGAGTCATTTGCTCTACCAACTGAGCTAAAGATGCAGATAGTGGTGCCGACTATAGGATTTGAACCTACGACCTATTGATTACAAGTCAATTGCTCTACCAACTGAGCTAAGTCGGCATGGTGGATTGTGAGGGGATCGAACCCCCGACCCTATGCTTGTAAGGCATATGCTCTCCCAGCTGAGCTAACAATCCATCACTTATGGTGCCGAATACAGGAATTGAACCTGTAGCCTATGCATTACGAGTGCATCGCTCTGCCCTTGAGCTAATTCGGCATTGGTGACGCGTACGGGATTCAAACCCGTGAATGCACGCGTGAAAGGCGTGTGTGTTAAGTCTCTTCACCAACGCGCCAAATAATGGCGGCCACAACAGGGATCGAACCTGTGACCAACCGGTTAACAGCCGGTTGCTCTACCGCTGAGCTATGTGGCCTGAGAATAAATTCTAGAATTTCTAGCCAATTTTAACAGCTTCCTTAGGATATGAATAACTATTTGCTTTGGGATGTTTTCTAACAAAAGTTAGCACAGAACTGGACACACCAACCTGTCCCATAACCATTAATAGACATAAAGCAAGGAAATTAAAGGTATTTCTATTTGGATCTAAAGCAATGTTAACAGTAGCTCCACTTGAAAGTCCAACTGTTCCAAAAGCACTAGTTGTTTCAAATAAAGCATCAATGAAACTAACTGGTTGTTCCATACTTAAAGGTAGAAGAACAGCTGTTAGTAAAACTGCAATTAAACTTAAAATTAGTACTAAAAAAGCATCTATTACTGTAGTTTGATCGATTGAACGTTTAAATGCTTTTACTTCCTTTTGACCTTTAAACTTAGCAACTAAAGCTAAAAAGATCACTGCTAGCGTAGTTGTTCTAATCCCCCCAGCAGTAGAAGAGGGACTAGCACCAATAAACATTGTTAATGCAATAATTATTTTTGTAGTTTGATTCTCACTAGCAACAGGGAAAACACTAAAACCTGCTGAACGGGATGAAATAACCATAAAAAAGAGCTGCATTACCCTACTTGCATTAGGGTTATTACCAAAAACTGACGCATGGATTGCTTGGTTAGATTGTGATTGCAATTGGGTATTTATTAAACTCTTTTTTTCATCACTAAAATTAACAATAGTGTTAGTTAAACTATCACTAGCAATAAATTCCACCATTAAAAGTAAGGTGAAAAAAAGCAGGATTACAACGATATTAGTAATTACTGTTAACTTGGTAAATAGACTAAATTGGTGTTTTGTGTGTCTACCATACTTAATCTTTTTTTTAATGGCTTCAAAGCCATCAAACAAACAAGGATAACCAATTCCCCCAAAGATAATTTGGCTAATAGTTAACCACTGGATAATAATACCAAGTCCATTTCTATAAGGAACAAAAGAACTACCCCCTATCAGATCTATCCCAGCATTATTAACTGCTGATAAGGAATGGAAAAAACCTGCTTGAAAAGCCTTATTAATATCATTAAAACCTGCTATTGTTTGGTTTGAATCAACTACTAAAGCTTTTAATTGAGTTGAAACTTTTGCATGATCTGCAAACAAGTTAGCAGGTTCAAAGCCTGGGATGAAATAAAACAAAATACCATATAAAAATCCATAAATTAGTTCAACGATAAACAGAAAGATGATAGATACTAAGATCATCTCACTAGTATTACCTAGCTTAGAACCACCTCGTTCTGATTGCAACATTAACTTTTCATAAAAACTGTATTGTTCCTTCTTGTGAAAGTTAAACAATCGTCATGTTAAAAAAGCAATAACAACAAACCCAATCCCTCCTAACTGGAGTAATACTGCTAAAACTATCTGGCCAAAGATACTGTATGTTTTTGATACAACAACAGTAGAAAGTCCTGTATCACTAAAAGCACTGGTTGATAAAAATAATGCATCTAAAAAGTTGTAATCGGTTTTCTGTTCAAATCTTTTTCCCTGTCAATCAATCCCATAACTAACCACTTTTTGGTAGTTATCTTGGAGTGCAATTGGCAAAAATAACAGCAAACTTCCAAACAAGATGCAATAGATATAAAAACAAAAAATTCGTTGAGTAATAGTCTCACCTCAGCCAATTTTTTTTAACCAAGTGGTGAGTTTTGCCATCTGGTTTTGTCTTCTTTTCATTAAAAGAACGCTATCAAATATAATTTTACCAAGGTATGAAACGTGCAGATTTTTGCATTATTGGTTTAGGTAGATTTGGGATGCAGGTTGCACAATCACTAAAAGAAAACAACTTTAACTTACTTTTAATTGATCTTGATGATAAAAAAACTGACACCGCCTCACAACAGTTTGATTATGTTATTTGTTGTGATGCTAGTAATCTAACTGCTTTAGAAGAGTTACAAATTGATGAGTTTGCTGGGGTTATTGTTGGGGTTACCAACATAGAAGCGAGCATCATGATATGTGCTAATTTAAGGGAATTAGGACAAAAAAACATTATTGCTAAAGCCAAAAATGAAGTACATAAAAGGGTGTTAAGCACAATGGGAATTAGAGAAGCTTTGATCCCTGAAAAGATTGTTGGTAAAAATCTGGTTATCCGCTTAATCCATGGGCTTGAAAATGAAATTATCAACCTTGGTAATGAGATAATTTTCATCCGTTCAGCAGTTAACAACAAGGCTTTTTTTAACAAAAGGTTAGGAGAGATTAACTTTAGGCAAAACACCGATGCTAACATTATCTCCATCATGCGCAGTAATAAAACTGTTGTTTTTCCTTTAGGACCAAATACTGAGATCCAACCAGGGGATATTATCACTGCAGTTTGTCAACAAAAAAGTTTAAATAAGTACTTAAATTACATCAATCCTAAAACCAAAAATAAAAATTAAAAAAAGGATCATCATGATCCTTTTTTTAGATAGCATGCTGTCAATAAGCCATGTTCTGTTTTAAAGTCAGTTATTTATCTACAGCAATTGCTGTCATTACTTTAATTATTTGTTCTTAAAGCAACATTCCCTTACCAAAATTTAGGTTTCTTGCTTGTGGAGTTTACCGCGTTTCATACCTGGTTTTCACCAAGCTCGTCTCTGTGGCACTTTCAAAACATCATCATAGTATTAACCTTAGACTAGTTATGTCGTTATGGCTATCACATCCTAAATCTTATCGCTTTGATTTACACAAACACTACTTGCATTCCAGCAAGTGCAAGCATGGACTTTCCTCTACTTTAAATATATCTTTAAAGCAGCAACTAACCGACAGCGTCAAAATTATAATTTGGTGTTGGGGATGTTTTGGGTTTGACATAATGCTGATAGACAAACAGTAGCATTGGGGTATGCCCCTTACAGCGCTAGGTTCAATAACCGACAAAGAAAATAACGAAGTGTTGGTAGATCCAAATTTGATCATTAACCAACAAGCAAGTGTTAACTTTGCTTTTGCATAAGTAGATACTAAAGCTACAGCTGGTGAATAGTCATAGTTTGCTAGCTGTCATAGTTTATGACTCGAGGTTAAATCGTTCAATTTAACCTTTAAAAATAGAACTTGTTGTTTCCATGATTGTTTTGTGATCAATTGGAAACAAGACAAAAATCCACAAAACTAAAATGTAGAAGCTGTTTGTTGTGTCCTTTATGGAAACGGGTTCGATTCCCGTCATCTCCACCATTTATCAACTTTAAGTTGGCCTTACACTCCCCTAGTTGGGAGTTTTTATTTTGCCTTACTCTTTTTAAAGAGTTGTTTTAATTTGGTTTTGTTGTAATTAAAAGCAAAGATAGCTATAAAAAAGAAAGAATAAAAAACTAAGCTAATCGCTCAAGCGGTAGCAAATACACCAAAACTATTTAATTTAGAGAGATAAACACCATTGTTATTTTCAACATTAAAAAACAAGCTAAACCGCGTTGCTGATTGAATTTTTTGTGTATCTCTTTCAAAAAACTGTTGGTCTAATAACACCAAAAATAAAACCATTAGCAAGATCCATAAGAGATAAACAACAATACTGATAAAAAATCCAGTAGTATAGCCAATGCGCTCTCACTTAGTAGTCTGACCTTTAAAAAATAATGAAACAGTTCACCTAAAACTTAGAAAAACTAATAATGCTAAACTAATTAACCCAAAAACTAAATTAGCAATTCCATATTGACTAATCTGATAAGTAATACTAGCTTGAATAATATCAGTTGGTTTTTCAACAGTTCAAAAAAGGTAAAACTGATTAAAGCCGTTAACTTCGTTAAAAGTGAATTGTTTAGTGTGAAAAACGCCAGCTAAAAACAAAACGTTGTTTAGAAAAAAGCAGAGAAAAAATAAAAAGTAAACAAAAGTATCAAGTAAGCCAAACCTGCTGTAAAACAGATAGTTACTGTGTTTGGAAACAACTTGATACTCAGCGACATTTATTTCACTGAGTTTATCAGATATCTTATTAATCCAACCACCACCACTATTTTTCTTTTTAGAAAGCACGTGGGATAGATTTAGATAATCAAACTGATTTTTCTTTTTTAACAAGTACCATATCTTCTATCCTAATTCCACCAAGGTTAGGAATATAGATCCCAGGTTCAATGGTTACAACCCCATTTTCACACAATAACTTGTTGTAAGATTGGGAAACATTTGGCATTTCATGGATATCAATACCAACGCCATGACCAGTACTATGCACAAAAAAGTCTTTAAACTCAGAGTTTTCAATAATATCGCGGCACACCTTATCAACTTGTGAACCTGTTAAAGTAGTGTTTACTGCATTTATACCAGCCAAATTAGCCTCTTCAACTTTTTTGTATGCAGATAATAACTTTGCACTTTTAGGTTTTTTACCAACTAAAAAGGTTCTTGTAATATCAGAGCAATAACCGTTGTAAATGGTGCCAAAATCACAAGTGATAAAATCACCTTCCTTAACTATTGTTTTGGTTGGTTTGTGATGTGGGTTAGCACCGTTTTTACCAGTAGCTACTATAGGATCAAATGAGTTTTTAGCCCCACCCTGCTTCACAAGCTCATTAGTAATCCATTGTGAAATGAAAAGCTCAGTCATTTTTGGTTTAATAAAACGCTTTAACTTAACTGCTACTTTTCTTGTAATATCAACTGCTTTTTCAATGGCTTGAATCTCACTAGGCAACTTTACTCTTCTAATCTCTTGGGCATTAATTACTGTGTATTGTTTACAAATAGCTTGAATTCAATCCTGATAGTTAAAGGTAAGATAGTCACCCTCAATTAAAAGGTGATTGATACCATTAGATTCACAAAAAGCTTTAACTTGTTTAAAACTAACAAATAATTCAACTTCAACAATAGGATTAATAAAGTTCCTTGCTGCTTCATAATACCTACCATCAATAAACAATTTTGCTTTATTGCTGGTGATGATTAACCACCCTGCACTACTTGGAAAATTAGTTAATCAAAAGCGGTTTTGATCAGAACCAATTAGGATAGCATCAGCTTTATTGGTTTTTAAAAGATCTTTAAGAACTGTAATTTTTTGTTGTAATTCACTAATCATTATTTACGTTTTACCTCTTTGTGCAATACTACCTTACGACAACGTGAACAAAACTTATTAAGTGCTAGTTTTTCTGGATTTTTCTTGACGTTTTTAAAGGTTAAATAATTAATCTCAGAACATTCATTACAACCTAGTCGTGTGCTTCTTTTAACAGCCATAATTAAATTATCTTAATTATTTATTGTCAATAAGTAAACTAATAGAAAAAGCATCAATGCCAGTGTGAACAATAATCACACTTGTAATGAAAGCATTTTCAATTTCATTTTGAAAGTTAATCTTATTATGCTCAATGAAATCAGTAATTATTTTTTTAATTTCATTAGCGTAATTTTTACAAAAAGAATAGCAAAAACCGATCCGTTTAATTTTGTAGTTATCACCAAACTTAGTTTTAACAAAACCAAATATCTTCTCAACCGCTTGACTAAAACTAAAAACTTTTGCTCCTAGAGTATTAACTCCCTTGTCAAACAAGATAATAGGTTTAACTCTTAGTAAAGTAGTAATGAACTTTTTCAAACCAGAGATTCTCCCACCTTTACGCATTTGCACTAGGTTTTTTAAAGTAACTGCAGAAAGGATATTTTGTTTATGTGATTCAACCTTTGCTTTAATTGTTTGATTATCACAACCCTTGTCAACCAATGCCTTTATATCTTCGACCAATCATTTTAGAGAAATAGCAATATCACTAGTTTCAAACACTAAAAATTCTTTGTCTTTATTTTGTTCACTAAGCTCTTTTGCTAATTGAACCAACATATCATAAGTACCACTTAAACCTTTACTTAAAGGCAGAAAAATAAAGCGATCATACTTAGTTTTAATCTCTTCAAAGATCTTAAGAAGATCACTTTGACGTGGTAAGGAAGTTGAGATGTTAAGTCCATGTGGGTTTTCTTTTAGAAGTTTATGAACATGATCATAATCAATTTCTATCCCATCACGAAAGCTTTTTTCACCATCAACAATTACTTGTAAAGGCAAGATGTAAACGCCGTTAATCTCCCCAGGTTTAATAGAAGCAGTTGAATCAGTGATGATAGCTGTTTTCTTCATGAATTAATCTCCTTAGTACCTTTGTTTCTTTAAATTATCTAGAAACTCTAGCACATAATCAAAAAAAAGTTTAGGCGCTGAGTCGTGTGGTGAATGACCAACACCATCAATAACCTTAAAAATAATCTTGTCACTTTTATTAGCTAGATAGTCTACAGAAGCTTTGGTTGGCGTGACAATATCATTAGCTCCTAAAATAACTAAGGTAGGTTTATTACCAATCATTTCATAAGCCCTTTCAAGTGAGTCATTACCATATTTAGCGTTTTGAACCATATCACTGTAAAGTGTTTTAAAGGTAGTTCTTTTTTTAAATGCATTGATAGCTATTTTCAAAAGTGATTTACGTTTTTCTTCATGTTCAACAAAATCCTTATGGTTGCTGTTATTACGCTTAAAAAAAGTATCTAAAATCCGCTTTTTATTAACTGAGAAAGAAGTTTGATTCATAGGAGCTACTAATATTAAAGCCTTAATTTTTAGTGGTATAACTTTGTTAACTAAAACAGCCACAGCACCTCCCATACTATGACCTATTAAAATGACGTTATTTAGTTTTTTTTGAACAATAAAATCACAAACCAAATCAACAAAGTGGTTTAGTTTTAATTGATCTGTATCTGTTGATTCATTGTCTCCATGACCGGGAAAATTAAAAGTAAAGAAAGGCCACTTTTTCTTTTTAAACAAGCTAAAAATACGACTAAAACTGGCATATTCGCTTCCAAATCCATGTAAAAAAATAAAAACGTTTTTTCTTTTTTAGGTTTAAAGGCAAATATTGAGTTAAAAAGAGTATTTTTATTACTCGTTAGCATTGATTTTAGCTAAACGTTCTGCATATGCTTGGATGCGTTCTTTCTTAGAAAGTTTAGCGAGTTTATCATCCTTATTTTGGATAACAAAATCACGTTTTTTAATTCGTGGTTTTTTTATTTCAACTGGTTTATCTTCATCACTAGCTTCTTTGATCTCTTCAGTTTGATTAGCACTAATGTCATTGAAATATTGCAACTGATTTTGCAGTGCTTGGTTTTCTAAAAAAAGTTGTTCTTTTTCAATTTTTAATGTTTCTAGTTCATCAAGATGTTCATCACTGGTAACTGGAGTAAATTGAAGTTCTTTTTTAGCATTTTCAAGTGAATTTATTTGTTGTTTTAACTGATTATTTTCACTATCAGATGCACTTAAACTTTGCTTGGCTTTCTCCAATTCACTGTTTGTTTCTTCAAAAGCAGAGAGTAAATCTTGGTATTGTTCATTAGCATCATTCAATTTATTCTTTTTAAGCTTTTCAAAATCAGTTTTTAATTTGCCATAGCTTTCTTGTAAGATTTCATGATCAGCTTGGAGCTTTGCTAATAAGTTATTGCTATTCTCAGAGCCTGATAATTCATCTTCTAAATCATTAATTTTTTCATTAAGTGCTAAATTAACTTCTTCAAGCGTGGCTAATTTATCTTTAGCAACATTAAAAAGATTTTGAGAGTCTTTGAGTTGCTGTTGGAGATTTACTAACTGATTGGTATTTAAATTACTCTTCTCTTCATTCCATTCTTCTAACTCTGCAATTTTGTCTTGCAGTTGTTGATTTTGAATTGTTAAACCATTAAGTTTTTCATCGAGTGCTTTTTTCTGTTCTTCAAATGAATTTTTAGTGTTATTGAAGTCATTATCTTTAAACCTTGTTCACTCAATTTCATCTAATAAAACATCATTTTCATGTTTAAGTGCTGAAATTGTTTTTTGATAATCTAAGTTTAGCGCTTGTTGTTTCACTTGCAATTTATCAAATTTTGTTTCATTCTCATCAAAAAGTAGTTCATACTGATTTAAAAGATCATCATATTTTTCATTCTCATCATTAGATGTGTTGTCATGGAGTTTTTTCAGTTCATTTTTCAAAAAATCATTTTCTTGTTCAACTAGATCCAATCTTTGGTTTGCTTCTCTTGCTGTTAGTAACTGGTTTTGAAGTTCGTTTATTTGGTTGTTTAAAACTGGTAATAAAGCTAATTGTTGTTGAACATTACTGCTCTTATTTTTCAATGCATCTAATTGATCTGAAAGCAAAGCTTTTTCTTGTTTTAAATTCTCCAACTCTTCATTAACTTCATCACTTAAAGGATTAATAGCAACTGGTTTATTTTGCAGTTGCTCATATAAATCCCTATTTTCTTGCAAAAGATCATCCTGTAATTTCTTGGTATGTAAGTGCTTACTTTTTTCCTCAGATAACCTCCTTTTAAGATCATCGATTTCCTGTTCTAAATCAAATATTTGATCATTATTATTCTCTTTAAGATCATCGATTTGTTTTTTTAAATCAGCATTTTCCAATAAAAGCTCTTTGTTATTATAGTCATCCAATTCATCAACTTTTTTTGTTGGATGTTGCTCTTTTAAGATCCTTTTAACAACAAAAGGTTGATCATCATTCTGATCATATTGGATCGTTTTATGAACTGGATTGATAGCTTTGGTTAAATTTTCAATGGAATAGAAATCATCAAATTGTTCAGGTTCTTTAACCTTATCAGTTGAAAATATTTGAACTTCTGGTTTTTTTGTTTCATTAATCTCTTCACTAGGTTGGTTAAACAACTCTTCAAAAGATACTTGTTTAGTAGCTTCTGGTTTTATCTCATCCTTATCATCTTCAGCAGTAATCACTGATGGTTTTAGTTCCTGAAAACCAGTTTTAATTTCCTTGGTTAAAACTGATTGATCATCAACTTCTTGAAAAGCCATAGTTTGATCAACTGTCTGTTGCTCTTCCTCTCTAGTTTTAAGTTGATTTGCATGATGATTTAAAACATTCTTATCCCATTTTTTATCTAAAACCTTTAAATCTATCATCCAATTAAAAAAAGAAACTATTGACTTTTCTTTCTCAGCTTCATCAACAGTACCAAAAAATGTTTTCTCCAATAAATTTCTTTCAAAGGGAATAGCAGTAATTTTAGATGGTGAAAAAAGGATATTATTAGTTCTTTGTACTAATTCTTTTAAAAGTGTTTTTTCATCAAGCTCATTATCAAAATCACTAACTTCAAGTTCCTTATCTACTGCCATGGATTATGTTGGAATATTAGATAAATAAGTATATTATCTTAAACTTCTGGCTTTGTTCTTATCCGTGCGTTTTTTGATTTAAAGTAAAGGCTAATAGGTACAGAATTAAAACCAAAGTTTTCTCTAATCTTATTCTCTAAAAAACGTGCATAAGAAAAATGAAGATACTTAGGGTCATTGCAAAATAAAACAAAATGGGGAATTTGACTTTTGGTTTGTACTGCGTAAGTTATCTGCAAACGCTTGCCTTTAAAAAGTGGAGGTTGATTGTAAAGTTGTGCTTGCTGAATAACATCATTTAAAAGTGGTGTTGCAACCTTAGTTTCAAGTTGGCTTTGAATAATTTTAAGTTGTTCAAAAATAGTATTTAAGCGCTGATTTTTCAAAACACTAATAAACAAAACAGGCGCAAAATCGAGGTGTTTAAAATGCAATTTCAGCATTTTTTTATATGCATTTGTGGTGTTATTGTTTTTTAAAATTAGATCCCATTTATTCACAAGAATAATAACAGGGATAAGTGCAGCTTGTGCCAATCCACCAATCACTTCATCTTGTTCACTAATAGGTTTTGATCCATCTACCATCAAAAGGATAACGTTACTACGGGCAATAGCTAGCTTTGTTTTGATGTAAGATGCGGTTTCAATTCCCATGTTAATTTTGCCTTTTCTTTTAATACCAGCAGTATCAATCAAAAGAAATTTTTCACCATTAACTTTTAAAGGAACATCAATAGCATCTCTAGTTGTACCACTCTCATTTGAAACTAAAACACGATTTTGTTTTACAAGTTGATTAATAAGTGAGCTTTTACCAACATTTGGTTTGCCAATTACACAAAACCTTATTTTTGCTAAATCATCATTATTTTCATTAGGGAGTAATTGATTTTGTTTAACTAAGAGATCCATTAAATCACCAATTCCAATTCCATGAGCAGCACTAATAACAACTGGTCTTCCAAACCCTAAGCTGTAATAATCTTTTAATGTTTCTTCAGCAGTTTTAGGGTTAAAATTTTCAGCTTTATTTACCACTAGTATTACTGGTTTATCCTTATTTTTTTTAAGAACCTTAGCTACATAAAAATCATCGCTATTTAGTTGTTCTTGTAGTGACACTAAAAAAATAATAGCTTTGGCTTGACTAAGTGCTGCTTGTGCTTGTAATGCAATTAGTTGTTGTAATGGAGTTTGTTTTGCAATTAATCCACCTGTGTCAATAAAAGCTATCTTTCTTTTTAACCATTCACCAATTCCAAAGATCCTATCTCTTGTTGTGTTAGGAGTATCTGAAACAATAGCCATTGGCTTTTGAATTAAGCGATTAAATAAAGTTGATTTACCAACGTTTGTACGACCTATTATTGCAACAGTAAACACAAATTAAAGTTTAAAAACCTTCTTTGCTTCTTTGAGGGTTTGTTTTACTACTGCATCAAAACTTAGTTCAGAAGTGTCAAGATAAATAGCGTCCTGGGCTTTTTTTAATGGGTCTGCAGTTCTAGAACTATCAATTTGATCACGTTGCTTTAATTCTTGAATTAGTTCCTTTAGTTTTTTTTCATTTGATAGAGAAATTCCCATATCTTGTAATCTTCGCTGCGCTCTAATTTCAACTTTAGCATCTAAAAAAAACTTCAATTGAGCATTTTTTAAAACTACTGTTCCTATGTCTCTACCATCCATCACTATGTTTTTATTTTCTGCTAGTTTCTGTTGTTTAATAACTGCAATTTTTCTAATGTTAGGATCAACAGCTATTTTACTAGCAATGTTAGCAACTGATTGGGTTGTAATAACTGTTGTAATATCAGCATTGTTATAATACACAGCATCTTTCTCAAAGCGTCAGTTAATTTGATTAATGATTTTTAAAAAAAGATCAATATTTAATCTATTCACTTGCATTACATAGGCAAAGGCACGATACATTTTTCCACTAGAAAAATAAAAAAAATCAAGTTCTTCAGCTATTTTTTTAGCAACACTGGACTTTCCAGAACTACTTGGACCATCAATTGCTATTTGTCAATTCATAACTAAATTTAAACAAATGAAACAAAACTAACAACCAAAAAAATAAGAAAAAACAAAATGCTAAACAGCATGAAACTTATTAAAGTTATCACTAAGTTCTTAAAAATAGCCTTATTAGCATTAAACAAATTAGGTTCGATGTTATCAATGCTTTTTTTAAAAGCAAAACTTTCAGTTGAAAAAACTGTTAGTATCTTGTTGTATTCTTTTAAATCACTAGTTAAATTGCTTGTTTTTTTTAATCATTGTTTCTCATTAGCTTCATAAAATTTACTTAAGTGGTTAATCTTTTCTATTCCCTTATCATCACTAATTCAGCTTTCATAACCGCTAGAAGTTTGGTTATATGCTGCGTTTAACCACATCCTATCAACTTTTTCTTCTAATAATTTAAAGTCAGTATTTTTTAAATCAACTATTAATTCCTGAAGTGTTTTAAACAGTTTATTTGGTTCAGTGTGATGTTTAACTGGATTTTTATTAAATTCACTAAAAGGATGGTAATTAATCAAGATTTTCTGATTAATTTTATTAAGCTGTTTTTTTCAGCTTTCAGTGTTTTTCTGAGCGTTAATTAAAGCTTTTTTAACTATCTTATTGTTATCAAAACTTTGTCTATAAAAGCGAAATTTTTCTACTCTTCCCATGGGTTGGCATTATGAACAACAAACTGAATGTCATCATCTTCCTCACAAGCATTGAGAAAACGTGATAATAAAGTTTGCTGATTTCTATCTAAATCAACAGTTAAAAGAGGTACCAATTTAATTTCACTATCTACTATTGAAAAATTGTTTTTTTTCAACAGTTCCTTTGCATGAAAATAACTATTTGAATGCAAGCTAATTTCAAAACACTCTTCATCATAATCAACATCAATTAAGTTAATATTGTCCAAAATTAATAATTCAATTAGATCATCTTTCAAATAGTTATTTTTATTAACAAAGATAATCCCTTCTTCTTGAAAATTAATCTTGACGCTATTTGGCTTGGCTAATTGTCCTTTTAGTTTAGCTAAATAACCGTTTAAACTACTGAGTAAACGGTTAGGATTATCAGTTAGTCCAAACACAATAATTCCAACACCATTTGGTCCAAAAATCTCATAACAAAACTCGCTAATTTTAGTTGTATCTTTTTCACTACCATGGATATTTCTTTTAATTGAATCCATGCTTAGACCCTTAGCTAAAGCAAGATCAACTGCAACTTTTAGATGTGGATTTGACTGGATATTAGTTCCACCTTTTTTAACAGCTGAAGCTATTCTTTTTGCTAGTTTTTGAAGTTGTTTTGCACTTGTTTGTTGTTTTTTATTAGTTTGATTAGCAATTAGATGCTTACGTGGCATGTAAGATTAAAAATTATTAGCAACTTGGTCAACTTTGTGTGCAAACAGATCAACTACCTCTTGCTTGTTTTTATCTTTGTAAAGAATTTTAGTGTCATCAATTAAAACACTCTCAAAATGTGTAACACCTGCAAAACCAAACAAGCCCTTTAAATTACTAACATGATCAGCTCAAGGGTATCATCCTAATGGTGCACCTTGTGACGCTAAAACAACTACCTTAAGATTAGTAAGTAATCCCTTAGAAGCACCTTTAGTAACATATTTGTCTTGAAAGGTTTTATTAGCTACAAAGACATGATCAATAAAGTTTTTTAAACTAGCAGGATAATTAAAGTTGGTCATTGGTGCAAGGATAACAATTCCATAAGCTGTTTTTAGAGCATCAATATAATAATCACTATTTTCAAAACTAAAAAAATTATTAGCGTTTTGGGTATTATAAGATATCTTACCTACTGGTAATTCATTTAAATTTCAATCAATAAATTCAACACTACTATTTTGTTTTTTATAAGTTTGAAGGAAACGATCTAATAAAAGGTGGGTGTAAGAACCAGAAGGGGTTACAGAAGCATCAACAATAATAACTTTCTTCATAGCTAATAATTAAAAGTCTAATTTTTATTGTTAATTCTAATGAGCTTTAGTTAATTTAGCAATTAATTGGTTGGTTTCAGTAAGTTTTTTTTGATATTCTTCCAATTTTAAAAATTCACTTTTTACCTTTTCTTTGGGTGCTTTTTCTAGAAAGCTTTTATTTTTAACAATAGCTTGGCTACGTTTTACTTCACTTTCAAAAAAAGCTTGTTGCTTCTGTAGAGATTTAAGTCTTTGGGTTGTATCATCAACAATTTGGTATTTAAAACTAACTTTTTTATTAGTTTCAATTTTCAGTTCAATTCAACTAAAGTTAAAGTATTGTTTAACATCAACAGCATTTTTACCAGATAAGATAACAACTAGTTTTTGTTGTGAATTAAGCATGTACTGTTTTCTGTAATTGCGTAAGTCATTAATAGCAGCTAAAACAAGATCAAAAAGTTTGGGAATTTTAATTTTAGTTGCAAGAGGTCATGTTGCTTGCATAACACTTTTATTGTTAAATTGCTGATAAATACGCTCAGATAAAAAAGGAACAGTGATACTAAGCAAAATAGCAATATTAGATAAAACTGATTTAGCAGTATAAAAAAGCTGTGGTTTTAGTTGATTTGGTTCTTTTTTAATTGCTTCAATGAAAGTATTGCAAAAATCATCCCAAACAAATTTAACAAGAATTTGGTTTGCTAATGCTAACTGGAATTTATCTAGTAGCTTAGTTATTTTTTGAATTACTTTATCTAATTTAGCTAAGATTCAAGTTTCACTTAATGAAAGTTTGTCCAAGTCATAACTAATTTCTTGATCATTTTCTAGTTGGATAACAAACTTAGTAACATTCCACAATTTATTTAAAAAATTTCATGCACTTTTTATTTTTTGTTCGCTGAAAATTAGATCATCTCCTGGAGTGTGATTTGAACACAAAAATAAGCGCACTGCATCCGCTCCATAATTTCTAATAAGATCAACAGGATCAATGCCATTATTCAGTGATTTTGACATCTTACGATTTTGTTCATCGCGTACTAAACCGTGGATTAAAACAGTTTTAAATGGCAGTTTTTTAGTTTCAAAAAAGGAGTTAAATAACATTCTTAAAACTCAGAAAAATAGAATATCATAACCTGTAACTAAAAGCTCAGTTTCATGAAAAGAGTCATCCTGTTCTCAATTCAAACAAATTAAAGGTCAAAGCGAAGAAGAAAATCAAGTATCAAGTACATCTTTTGATCTAGTGTAGTTTTGTAAATTTTTTGAAGGTTTTTCACCAACAACTATTTCACCTGTATTGTTTTCAAATCAAACAGGAATTTTATGACCCCAAATTAACTGTCTTGAAATACACCATGGTTTGAGTTTATTCAACCAATTTGACACTTGCTTATTAAAGCGTTTGGGAATAAAATCAGGATATTTTTTTAAATATAAGTGATCTTTTAACTTTGGTAAATCAACAAATCACTGTTTTGAAAGCATGGGTTCTACAACAGTGCCACTGCGTTCAGAAAAACCAACATTACTAGTTAATGGTATTGATTTAACAAGTAATTTATTTTTTTCTAATCATTTAACAATTTTATTTCTTGCTTGTAAAACACTAAGGCCTTGAAATTTACTTGCATTTTGATTGAGAATACCGTTACTGTCAATGCAGCTTAGAAAATCAAATTTATATTTAGTGTTGATTTCATAGTCATTAAAGTCGTGTGCAGGAGTACATTTCAATATTCCTGTACCAAATTTAATGTCAACATAGCTATCTGTTACAACAAGAATTTGTTTTCCTGTTAAAGGGTTAACTACTAATTTATTTCAGAAATTAGTATAGCGCTTATCATTTGGGTTTACCAATAGACAAACATCAGCAAAGATAGTTTCTGGTCTTGTTGTTGCAACTATTAGTTCTTGTTTACTATCATTCGCTAGTTTATAAACAACATAATGAAGATGTTGATTAACAGGTTTATTGATAACTTCAATATTTGATATAGCAGTATTCAATTTTGTATCTCAATTAACAAGCGTGTATGCTTGATAAATAAAACCGTTTTCATAAAGGTTTTTAAAACAATTGTTAACAATTTTATTAGCTTGTTCTGAAAGCGTGAATTTAGTTTCAGATCAATTTAAGCAAACTCCTAAACTCTTTAGTTGATTTTTAATTATTTCGCTTTGATTTAATGCCCAATTCATGATCATTTCAGATTTTTTATCATCATCTGCATCAAAATATTTTTGATTTTCTTTTAATGCTATTTTTTCATATTTCGTTTGAGTAGCAATGCCAGCATGATCAAAGCCAGGAATTCAGTTAATACTAAATCCCTGCATCTTTTTAAAACGCATGATTTGATCAGTAATACTAACCTCAAAAGCATGACCAATATGAAGAGTACCTGTTAGATTTGGAGGGGGAAGAATTGCTGTAAAAGAATTGTTTTTATCTTTAGGTTTAAAAAAACCAGCATTATTTCAAATTTCATAAAGCCCATCACTAACTAAGTTGAAATCATAGTTTTTTTGAAAACTAAATTTATCTTTCATCTTCAACGAATAATTCGAAAATTTTTTTGTGAACTAAAGCAAGATTATGATGTGTTTTTGTACTTGCAAAAACACATTTATCAAAATTAACTTTTAAAAAATTTGCAATATTCTTAAGCGAAAAATATCTTTCACTTTGATTTAGTTTGTCAAATTTATTAGCAACAATCAAAAAGTTCAATCCTGTTTGCAAAATAATTTTAACCACTTCCTGATCTTGAACAGTAACAACTCCACTATCTACTATCAAAACAACACCAACTAGATTACTACGAAAATTTAAAAATTGGGTAAGTAAATTAGTAATAAAATCTTTCTTATTTTTGCTTATTTTAGCAAAGCCATAACCTGGTAAATCAACAAATCTTTTGTCTTTATATTCAAAGTAATTTAGTAATTGTGTTCGACCTGGTGTTGCTGAAGTTTTAGCCAGTTTCTTTTTAAAAAAAGCATTAATCAAACTTGATTTACCAACATTACTTCTTCCCATGAAACAAATCTCTGGGATATTATCTTGAGGACAATCTTTTAAATCACTTGCACTCTTCAAAAAATGTGCATCCATGTGTATTTTATTTTTGGTTTTTAATGGAGCGCATTATCTCTTTTACCTGGGTTTCAGATAATTTTCTACCCATTTTTGCATACATTGCTTTAATTTGATTTTCAGTAATAGGTGGATTGTCACGCATCTGTTTTTTAAAGATTTTTATAGAAATAAAATAACCAAGAATCATTCCCACCAAAAGCGAAAGAGGGATACCTAAACCTAATGCAAGTGCTAGATCATTCATAGCTATATATTAATAATTAAACATTTTAAAAGTCTTATAAAATTAAATAATCGAATTAATGGATAAACTTGTTAGTATATTAGTTCCTTGTTACAAATCAAAACCTTTTTTAAAACGTTTTTTAATTCACTTTTAAAGCAAGATCTTAATCAAGCTAAAATTATTTTTTTCAATGACAATGTTGCTGATGAAACCTATGAAGTTTTGCAAAAATTCAAAAAAGAACACAATAACTTAGCAATTGAAGTCTATTGTGACAAACAGAATGAAGGTATTGGAAAAGTGCGTGATAAGCTAGTGAATCTAGTAACAACACCTTATTTTTATTTTATCGATCCTGATGATTGTTTTAACAACAAAAATGTCATAAAAGAGATTGTTGAATCAATTAAAAAAGAAGATTTTGATCTTGGTGTATTAAAAAGTATGGTCTATTTATGCTTCTTAAAACATGATTTCATTATTAAATTTTTGCCTTTAAAAGGTATTTTTCAAGGCAGAGTAAAATTAATTAATAATAACAATGTTAATAAATTAAATTACATCAAAAATAATGATCAATATATTTGAAATATTGTTATAAACACAGATTTTTTTAGGAAGCTAAATTTAACTTTTGAATCAAGGTTATTTGAAGATATACCAATCTGATATCCGATGTTTTTTTCATCACAAAAAATTGTTTTTATTGATGTGATAGGAACAAATTATTTTATTCGTAATGATAGTTTATCAACTACTATTAGTGCTCCACGCTACTTAAATTTAATCCAATGTTATGAAAAGCTATATGTAAATCTCAGCCAAAATGGTTCTCTTGCAAGTTTTATTGATCCAAATCATAAGATTGAAGCTAGGTTTTGAAGAAGGCAAATGTTTGTTTGATTTGCACTTTTCAGCTTTGAATACTTTAAGAAAAATTTTTCTGAATCAAAAAAAATTCTTGAAAAACTATTTGTTTTTTTGGAAAAAAATGGAGTTTATGAACGTGTTTTTCAAACAAAAAATCAAGGTATTTACCATATTTGGGTACAGCGACTAAAATATTTTAAACATGTTTTGGAATCTAAATCAGATAACTAATTAAGTTCTCTTTAAAAAATCAATGAGATTAAAGTTATTTAAAGCTTATTGTTTAATTTACTTAAATTAAGAATCTTTATCTTGTTAAATTAATACTTAACTGGTTTAATGTCTGCAATTAAATTTAATCCTAGTTCATTCAGAAAAAACTTTAAATGGTTTGAAAATAACAAAAATTGGATTAATTTTGATAATGCTGCTACTTCCATTGCACTTGATGTTGTGGCTGAAGCAAGCAAAGAATATTACCAGTATTTTTGTGTCAATCCTCATAACAAAAATCCTGAAATTAACCAAAAACTTATTGCTATTATTGAAGAAACAAGAGATTTATTAGCAAAATTTTTCAATGCTAAAAAAAATGAAATAATTTTTACAAGTTCTGCAACTGAATCGCTTAACTTATTCGCCTTTGGATTAAGCTCTTTAGTAAAAAGTAATGATGAAATCATTCTCAAAGAAGATGAACATGCTGCTAATGTTTTTCCCTGAGTAAATCTAGCAAAAGAAAATAAAGCCAAACTAAAAATAATTAAAAAAACACCAAATAAATCTTGAACTGATGCTTTTTTAAAAGCTTGTACACCATCAACAAAACTATTAGTTATAACTGCAACATCTAATCTTTTTGGAAATAGTATTGACTATGAAAAAATTTCTAAACACTTAAAAAAAATATCACCAAATAGCTTTATTGTTGTAGATGCAGTACAAGCTGTACCACACCATAAAATCGATATTACAAGTGCTAATATTGATTTTTTAACTTTTTCTACACATAAATTTTATGGACCTACTGGTCTTGGCATTGCCTTTATCAAAAGCGAATTACAATCACGACTAAAACCCTTTAAATTAGGTGGTGATATTTTTAAATCATTGGATAATAACTTTAAGATAATTTTTAAAGAAGGTCCTTCCAAATTTGAAGCTGGAACGCTAAATATTATGGCTATTTATGCTTTGAATAAACAGTTAAAATTCATGCAAAAAGAATTTAATTTCAGTGAAATGGTGTTTTACAGCAAACAATTAAAAAATTTAGCTTATCAACTGCTAAGTCAAAATCCTAATATCGTTTTAGCTAATCATGATCAAGATGTTCCTATCTTTGCTTTTAAGCATAAATATATTAATTCTGCAGATCTAGCAACTTTTTTAAACATTAAAAAAATAATTGTTAGACAAGGATCCATCTGTGTTGGTAAATTTAAAAATAAAGAGAGTTTTTTACGTGTTTCTCTACTCCATTACAACACAAAAGAGGAATTACTTTATTTAGAAAAATTATTAAAAACTAGTAAGAATTCCATTATTAATGAACTAATATATTAGCTAATGGATATTAGAGCAAGAACAAAAATTATTGATATTTATTCAAATTTAAAATACAAAAAACCTCTCAAAAGTTTTCAAAAAATTTTAACAACAAGTGATAGTGATAATTGTGAAGACTTTTTTAATATAGGTTTAAATATTGATAAAAATAAAATTACTGCAATTGGTTTTGATGGTGATGGTTGTATTATCTCTACAATTGCAACTGAATTAAGTATTAAAGCAATTGAAAATAAAACTATTAACCAAGCAAAAAAAATATTATCAGATTTAATTGCAACTTATAAAGATAAAAATTCAGCAAACCAAGTTGTTGAAGAATTAAAGCTTTTAATTGAAATGAATGTAACTGAAAAAAGGTTACAGTGTTTGCTTTTAACACCAAGCAACTTATTGCAATGGTTTAAAAATTTTTAGCATTAATAGCTAACGTTTGTCCTAGGATATCATATAAGCGTTTATCACCAACTAAAATCTGTTGATGTTCTCTTTGGTGTTGCTTTTGATTTTCACGAATTAAATCTAAAAATGCCAACTCTTGAGTGCCTTCATCTAAAGCTTGTTCAATAAGTAAAGACATAAAGATCTCTTTCTTTAAACCTAATGAATTAGCTTTATTATTTTTTCAATTACTAGAAATCTTATTTACATCATCATTTGTTATCTGAATAACATAACTAAGAGTCTGGATACTAGAGTCATTTGTTAATGGAACTGTTTGCACAGTTTTATATCCTTCTGTATTGGAACCATCTGTATTTGCAGCTTTAGATAAACCAACATAACCTTCAAATTTATGAAAATAAAGATCATCTAGTCTATCAACTACATTTAATAAAAACTTTTTTTATTATCAAAACTTATTAAAGGTTCACCTCTAGAGTTAAAGCGACTTAAATTATTTGTATCAACATTAACTGAGCGATGTAATAACTCACCAAAAGCATTAAGTTCAGCATCAGAACTGGTTTTATTAATCAAGCTTCACAAGGTAAACTTATCCTTATATTGACTTAAAGCGCCTGCTTTATCAGTTGCTGTAATTAACTGTCCGTAATTATTAAAAAGCTGTTGTGTTAAACCAGCACTAGCTGTTGAAATACTTTGATTGGAATTAATTGTTAAACCATTAAATCCATAAAAATATTTTTTATCACTTGATCCTTGTGATTTATTAGATAAAGCTACAGAATTATTAAACGGTTCATCAGTAAATCTATAAGTGTTTTCACTTTCAAGCAATGAATCGGGTTGAATTGGTGTTTGTTTATCATTGAATCAGTTTATTTCAGAACCTTGAGTATAGTTAGGATTTTGTAAAAACCTATTAAAAGGGTTTTCATTAGCTGATGAAGTACTGGATTTTGTTTTTTGACTAACAGTAGCGCTAGGGTTTTTATCATATCCTACTGTTCATGAAACAAATGCTCTAGTGCCAATTGGAATTGCTTGTTGTAAAAGATTCTTATAGTTTTGAGCACCATTTTTAACAAGTCACATTAACGCTTGCAAAAAGCGCATGTATTTGTACTCAGCATCCTGATTAGGATCTTCCAATTCCTTTTCAAATGCTTTCTTTGCTAAATCTAATAGGGTTTGATTAACACTACTAGAAGTATGAGATTGGGCAGCTTTATTTTGCATTTGCAACATAGTTGAAGTTGAATTTACACTACCCAAATCCTTATAGTAACCAAAGCGTTCTTTACTAGTTAAATTTTGTCAAGAACGGATATAAAGTGCCTGTTTAACAAGATCATTAAAATCAGAAAATTCACCTTTTAATTCTTTATTTGAAGTATCAAAATTATTAGTGAATGAATTAGTGTTTTTAAAGTACCAATTTTTAAAGGTGTTTGCAACAGTACCTGTTCTAATTCCTGAATCAGTTAAAAGTGATAATATAAGATCAAGATCCAAGTCATTTAATTGTGATGATTTTAGGGTAATAATCTCTGAATATTGACTAGAAGAATAAGTTGTAGCTTTTCATTTTTCAACTAATTCTTTGGCTTTATTTTCTACTTCAGTTGTTAAAGTCTTTAAAGTTTGATTATTTTTTTCACGAGTTGGTTCATTATCAGAAGTAATAGCTGCAGCAGTGGTTTTCAAAGTTAAAGTTTGAGCACTACTTTGATCAGAGTTGTTATTAAAAAATTTTGCTAATGAAGGATAACTACCATCATTTGCTCTTTTATATGGAAGAGGTTGACCCCAACCAATTGAACTTTCTTTAGCTTCCTTTTGAAGTTTAATGTAGTTGTTAGCACGATCAACAATTGCTTGTTCTAGTTTTTCTATATTAGCTAAATCTGTTCTAAAGTTATTAAGTTTATTAGCAGCTTTAATATAAGACTCATAACTTGATTTTATTTGTGAAAGTGAATTATTAAAATCTTGTTGTTTTTTAATAGTTTCAGATAAATTGGTATTGTTTTTTAAAAGTTTTTTAGCTAAATCACATACATCTTTTTGACCGTTATTATTAGAAGTTCCATTTTCTAACATAGTTGTTAAAGCTGTGTAAAGTTTTAGTGTTGTATTTCTATTTGCTCAACTTCTAAATTCGCTGTTAGAACCAAATAAATCAACTGAAAAACTGACATTATCTTTAGAGAAATTATTAGTTTGTCCTTGTAAAAATCGCATTAATAAAACTTCTGCTTGTTTTTCAAAATTACGTTTGTTAGGTGATGAATTCTCTAAAAAATAATTACCTCCATCAATATGAAATGCATATATGGCATCATCACCTCTAACATAAACAGGTTTCTGTTGCTGTTTTTGAGTTGTCTTTGAACTTAGACTAACTAATAAATTACTTGTTACTACTTGTGCTTGAGATGAATTTGATTCCATTCTAACTGCATCAAAAATAGTTGCAGTTTGACTACTATTATTTAAAGCTTGTACTTGTTTTCAAAAAGACTGCGTGTTACTACTATTATCACTACCAGAAGAAGAAAGCGAGGGATTAATTTTTAAAAATTCACTTTTAACATTAAAACCATTATTGTCTGAAGTAACTTCTTGACTTTTTGTCATAGCACTCTTCATCATAACTGTTGTTGCACTTGAACTTTTAATAAATTTATCAAGAGGATTTAAATTGCTACTAGTTCCACCTGTTGTTGTTGTTCCACCTGTTGTTGTTGTTAATTCTGTTTTATTATTCAGTAAGTAATGGTATTGATCAATAACTGCAGCAGATAAGTTAACAGTTTTTAAAACAGAGAGAATGTTATTTTTACTAATTAAAACTTTGCTTGCACTATACTTGTTTTCAAAGTCAAGTAAATCATTTCTGGTATTAGTAGCAGCTGAACTTTGTGATGAACCACTAGAATTAGCAGTTTGTAATTGTTTATCAAACTCTTCAAATAATTTTGCAGAGTTTGTTTGACCACCACTTTCACTAGGATCTTGAAAAGCAGGAAAGTTATAAGAAAGATTTAGATTGGAAGCTCCAATTGCATTTTCATCATAAATACTGCCAAGTCCAGCAATTGGAGATTGGTAATTGAAAGAAGCTTGATTTAATAAAAATGGGTTTTCTTCTATTAATCACTGTGAAAATAAATAAGCTTGAAGATTGGTAAAAAAATCACCATTTACATCATTTAAATTGCTGTTAATAAACTTAAGTTTATTTTCTGTTTTTTCAATGTTTTCTTTAGTTGTTGCAACGCTTGTATCACTTGAATTCACTAAACTAACATTTGCACTAATGTTATTGAATATTAAAGATTGAAAATCAGTAATTAATCTATCATAGAGTTTAAGTTTTTTTCAACTTTCTTGATTACCACCGAACTGATCATATTCTTTTTGTACTTCAATAGGTCACTTTACTCTATCAGCACGTTTCTTTTCATCTATCAAGTTATCATGTTCATCTTCAACATTTTCTTCAAATATCTTTAATCGATTAGAGATGTTTCTATCTGCATTTGCTTGGTATCATTTTTGTAATGCTGATCCAAAACTAACACGTAAGATAGAATCAACACCACTACCATCATTTAAAGCAGACTTTAAAACTACATCTGGGTTTATGATATTTTGATCACTAAAACCAATTAGTTTTTGAGATCGATTGAGAGTAGGAAAAAGATTAGCAGTGTTTATTGTGGAACAGGCTGTAATTATTGCTGTTGCACCCACTCCACTAAATGCTAAAGCAATTAATGATCAGCGCTTAAAACGATGAGCCATCTTATACTAGCAGTTTGATAATTATCACATTTAAAAATCTAGCTAATATAATTGCAAATCTAGATTATTAAAAAATGTATATTAATTATAAATAAGTTAATTTTTCTAATTTAAGCATTAAAAAAGCTTAAATCTCTCTAATTAAAATGCTTTACCAGAAAATCAAAAAATTAGTTTTTAGCTAGCTGTTTGTTGAAATGAATTTTTATTAGCCAATTGTTGAACAGTTAGTTCTTTTATTTGTTCAAATAATGCTGGCTTTTCTGATAAAACATTTAATAATTTTTCTTTACCAACAGCAATGCTTTCATTGTTAAAAGAATAAGAATTTCCAGCTCTTACAACAACATTAAATTTAAGTGCTAAGTCAATAACTTCATGTTCATGTACAAAACCACGGTTAAACATGATTTCTAATATAGCAACACCAAAAGGTTTAGCAATCTTATTTTTAGATACCATTACTTTTGTTTTTATGCCAACATAATTGTTGAATTTATCCTTAAGTAATTCAACACGCTTAGCTTCCATTCTTAATGAACTATAAAATCTTAGAGCTTTTCCTCCTGTTGTAACTTCGTTATTTCCAAACATCACTCCTGGTTTTTCGCGTAACTGATTAATGAATAAAACACAAGTTTTAGAATCTGGTAATATCGATTGTATTCTTCGCAAACCTTTTGACATCATTCTTGCATGCAAGCCAATAGTTTGTTCTTCAATGGTGCCTTCTAGCTCTTGTTTAGGAATTAACGCTGCTACAGAGTCAATAACAATTAAAGATATCTTGTTTGTTTTAATTAATGATTCGATAAGAGCAAAAGCGTTTTCACCATGCCTAGGATGAGCAATCAAAAGTTTATTTAGATCAATACCAATTGATTTAGCATATGCTAAATCAAGTGCACCTTCAGCATCAATATAACAGGCTGTTTTACCTGCTTTCTGAAAACTAGCGACTGCATTTAGTGCAATAGTTGTTTTTCCAGATGATTCATTTCCATATAGTTCTACTATCCTACCTAAAGGCAGACCACCAGACCCTAATGCTTCATCTAAATTTAAACTTCCAGTTGAAATTGTTTCAATTTCACTGTTTTTCTTTGCATCAAAAAAATCAAAACTTGTCAAATTATTACTTTCAATAAAACTACTATTTTTTTGAGTATTTTTCTTATTAATTATTTCTTTTTGAGCCATCTTTCATTACACCTTATAAATAAGTGCAAAAAAATAAAAATGACATTTTTTTATTAGAAATTTTGTTTATTTTTTAATTTTTCTTAAAAAAATCTCTTTTTAGGAGTTTATTTTTTTATATTAGTTAACTTGTTTGGTTTGTTGACATAAACACTCCTAAAACTCCTATATATTATTAGTAAATATATAGATTTTTGTTCAAGTCCAAGGATGTGAAGCTAAATTACAGTTCAAGTGGCTCAAATACCAACACAATTAGTTTTGATTCAACTAACAACAAACCATCCTACATCGTTGAATTTACTAATTCTACCAACATTGGCATCAAGTGAAGCGTGGTGAAAAAGTATCAGTTAGATGTGCCAAGTGTTACCAATGAGATGAACCAAGTGTTGCAAGAATTGATCCTAGAACAACCTTTGACTAAATATACCTTAAACAGTAGTTTGGCCAAGCAAAAGGGTAAGACACAAGCAGTGGTGCATCTTGGTTCAAATATGACAAATAATTGACGATCGATGCGCAACTCCATCAGTCTAAACAACAATCCCTCTCCCAATGCATCAACCGGATTTAAACTCACTACCGGCAACGCTTATAGAAAATTGGATCAATCCTGACCAATTTACCAACCAATTGATGGGACCAAGCACGGCAAAGGGAAGGATAGTAATGGGTGGAGTTCAACAGAAGAAAACAATGGCAGCAGGGGATGCGCCCAGTGTTACAGGAGGGGGAACATCAGACAATTCTTCAAAATTCCAAAGTTACCTCAACACCAAACAAGCGTTAGAAAGCATCGGTATCTTGTTTGATGGGGATGGAATGAGGAATGTTATCACCCAGCTCTATTATGCTTCAACTAGTAAGCTAGCAGTCACTAACAACCACATTGTCGTGATGGGTAACAGCTTTCTACCCAGCATGTGGTACTGGGTGGTGGATAGGAGTGCAACAACTGATTCATCATCAAAACCCACCTGGTTTGCTAACACCAATTTAGATTGGGGAGAAGACAAACAAAAACAATTTGTAGAGAATCAGTTGGGGTATAAGGAAACTACCAGTACCAATTCCCACAACTTCCATTCCAAATCTTTCACCCAACCTGCATATCTGATCAGTGGCATTGACAGTGTCAATGATCAAATCATCTTCAGTGGTTTTAAAGCGGGCAGTGTGGGGTATGATAGTAGTAGTAGTAGTAGTAGTAGTACTCAAACCAAAGACCAAGCACTGGCTTGATCAACAACAACTAGCTTAGATAGTAAAACGGGGTATAGGGATCTAGTGACCAACGACACGGGATTAAATGGTCCGATCAATGGAAGTTTTTCAATCCAAGACACCTTCTCATTCGTTGTTCCTTATTCGGGGAATCACACAAATAATGGAACAACTGGAACCATTAAAACGGCTTATCCAGTGAAAAACACAGAAAAATCAACTGTCAAGATCAATTCCTTGATCAACGCTACGCCGTTGAATAGTTATGGAAGTGGAAGCAACTTCCACCTTCAACCCCACCCAAAGGTTGCAAAGTGGGAGTCCAGAAAGCGATTCAAGTAAAACAGGGGAGAAACTCAGTGAAGCAACTTCATCGATGAGTAGTGGGGCTACATCCACTCGAGCCAAGGCCCTTAAGATAGAGGTGGAAAAAAAAACGACTTTGCCAAAAAGCCGTTGCAGCATAAGAACAGTAGTGGGACAGAGGTGAAGTTAGCTGCGAGTGGGGAGTTTGGTGACAACAAAGCCTGAAAACCGGTGTTGACTACCGAGCAAATAGCAAGAGAGAAGGGGATGGGGGCTTCTTCTCCAACTCCCCCTCCCCTTCAACTTCTGCTTCTTCTTCTTCTTCTTCTTCTTCTTCTACTTCTTCTTCTTCCTCAACCCCACTCCCCACTTTTTCTAACATCAATGTTGGGGTTAAATCAATGATCACTCAACACTTAAACAAAGAAAACACCCGGTGGGTGTTTACTTCTGGTAGTTCCCCAGACATCTGAACGGGTGCAGGTTATAGAAACAGCTAATGACAACAATAACGGTATCTCCTTTGAAATGGTGAAGCCTAGCAATAGTAGTACCCCCTTTAATCCCAATTCTTCTGAAAACCAAGTCACACCATCAGGTAGCTCCCCCAAAAAAACTACATATGATTTTTTACCCAACAGTATCAGTCCCACCAGTGACTGGATCAATGCGTTGACTTTCACTAATAAGAATAACCCGCAACGAAATCAGTTGTTGCTCAGAAGCTTACTAGGAACGATCCCGGTATTGATCAATAAGAGTGGAACGGGAGATGAGTTTAACCATACGAGTGACCAGAAGTGGGATAAAACTGAGACAAAAGATGGGAATTTCCCTGGGTTTGGGGAGGTGAATGGGTGAAAAATAGTAACTATTTTTTTAAATTAATACTCTTCAGCAACTGTTTTGCTACCTAAGAAAGTTAACTCTTCATCAGTTAAAAATCCTGTTCCTGCTGGAATTTTTCCACCAATAATAACATTCTCTTTTAAACCTAAAAGATAGTCTACTTGGTTCTTAACAGCAGCATCAGTTAGGATTTTTTTGGTATCTTGGAAGGATGCAGCACTTAAAAAGGAGTTAGATTTACTTGCCGCTTCATCAATTCCAAACACCTGATTAATTGCAATAACAGGCATCTTTCCAGCTAAAAGTAAGCTCTTATTTAGTTCATTGAGATAATGGCTATGCACTAACTGACCAACAAATAAATTGCTGTTACCTGCATCTGTTACTTGCAACAAATTAGTTAGTTGTCTGATAATAATTTCTACATACTTATCAGCAATATCTATCCCTTGAATCCTATACACTTTTTGGATCTCTACAATCATGTATTGTCTTACCCTTTGGATACCTGCAATTCGCAAAAGTTGTTTAATATCAACAGAACCTTCTGTAATCTTAGAACCTGGTGAAACTTGATCACCAACATGAACACGTATTTGAGCACTGAAAGGGATAGTATAAATCCTCTCATCAACGTTTGATTTAATCACTACTTCCTGAGCGTTTTGAACAGTAGTAATTGATTTAACTGTTCCTTTCACTTCAGAGATAACTGCTTTTTCAAAATCTTTAGGAGTAACTACTTCAAAGATCTGTTTTAAACGTTCAAAGCCTTGTGCTAAGTTGTTTTCAGTTGAAACTCCCCCAGTATGGAAAGTACGCATTGTCAATTGGGTCCCAGGTTCACCAATTGATTGAGCAGCAATCACCCCAACAGCAGTTCCCAATTCCACCAACTTACCAGTTGACAAGTCAATGCCAAAGCAGTATTGACAGATACCATTTTCCTTTTCACAATAGATAACAGATCTAACTAAAACTTCCTTAATAGATGTTGCACAAATCTGTTTAGCTAATTGCGTTGTAATAAGACTGTTAGTTTCTACAATAGTTTTTTGTGTTTCAGGATCAACTATAGGAGTAATTGAGTAGCGATTAACAATCCTGTCAAATAATGATTCAATCAAGGATTTGGTTTTGGTTTCAACAATTGCTTCAACAACAATTCCTTTTCTTGTTCCACAATCATCATGGTTAATAATTAATTCATGAGTAGCATCTACTAGCTTTCTTGTCATATAACCAGACTTTGCTGTTTTCATTGCAGTATCTGTCATCCCTTTTCTCGCTCCATAAGAAGAGTTGAAGTATTCATTAATGGTCAAACCTTCAAAAAAGGAGTGTTTAATAGGAACTTCTATCGTATCTTTAATGATCTTAGATTGGTTATTTCTTTCATAGTTAAAGCTTTTAGACATTAAGCCGCGCATCCCAAATAACTGGGTAAAGTTAGAAATGTTACCTCTAGCGCCTGAATCAGCCATTACCACAATGGAATTATCACGGTACTGTTCTTGTTTAATTAAGCTTTGGATCTCATCAGATACTTTTTCTTTCACATTGTTTCATAACTTCACAACGCGTTTATAACGTTCATCATCGGTTAACAAACCCTTGTTATAAAACTGTTTGTATTTCAGCACCTGTTGATCAGCACTATCAAAGTAATTTTGTTTATTAGTGTACTTAGGGATATCAAATACTGAAACAGTAGTTGAAGAAAAAGTAGAGTACTTAAAGCCAAGTGCTTTGATATTATCAAGTGTTTTAGGAAGATCTTTAAATTCCAATAAAAGATAGAGTAGATCAATTAACTTGGAAATAACCTTTTTGGAAAATGCAGGGATAATTTGATGCTTTAAAATTGCTTGTCTTACATCTTCTCCCTGTTTCACAATGAACTTTTCAGGACATGCATTTTCATCAAATTCCCCGTCATTAATATAAGGAACATTATTGCCTAAAACATCATTAAAGATAATCTTACCAACTGTTGTTATTAAAGTTCCTTGGCATGCAAACTTCTTGTTGGGAAATGCTTTAGTACTTATCCCTACAATAGCATGTAAATGAACCTTTTGACTTTCATAAGCAGCACGTGCTTCATAAACTGTGCTGAAGATAATCCCCTCTCCCAACTGTCCTTTTCTCTCTGTTGTTAGATAATAGTTACCTAAAACCATGTCCTGAGTAGGAGTTACAATGGGTCTTCCATCTTTCAAACCTAAGATATGTTTTGATGCAAGCAGAACAGCTCTTGCTTCATTAACTGCATTATCACTTAAAGGAACATGAACTGCCATCTGATCACCATCAAAATCAGCATTAAATGCAGTAGTTACTAATGGGTGTAATCTAATTGCTTTACCATCAACAATTCTTGGTTCAAATGCTTGGATCCCTAATCTATGTAGGGTTGGAGCACGATTTAGTAAAACAGGACGATCTTTGATAACATCATAAACTATTCCCCAGATAAGATCATCCTGGTTTTTAATCATATCTTCAGCTTGTCTGATGGAAGCGGCAATTGGTCTAATCTCATTACCATTTTCATCAAACTTATTGATCAATCCATGGATAATAAAGGGTTTAAACAGCTTTAAGATCATTAATGCTGGGATCCCAACTTCATACATCTTCAATTCAGGGCCAACCACAATTACACTTCTACCTGAATAATCAACTCTTTTACCAAGTAAGTTGTGTCTAAATAAACCTTGTTTTCCTTTTAAACGATCTGTTAAAGATTTCAATGACCGTTTGTCTTTGGAAAGTGATGGTTTATGACGTGAAGAGTTATCAAATAAAGCATCAACAGACTCTTGTAAAAGTCTTTTTTCATTGTTAACAACAATAGAAGGTACAGTACCATCTTCCAAAATCCTTCTTAATCGGTCATTTCTAATGATTACCCTGCGGTAAAAATTGTTGATGTCACTGGTGGTAAACTTAGCACCATCAAGTTGGATAATAGGTCTGATATCAGGGGGGATAACTGGAACAGTATGTAAGATCATGTTTTTAGGATGAAGCTTAGAATTTCTAAACCAACTAATAGTTTCCAGTTGTCTTAAGATCTTCTTAACTTTAGCATCTTCAACACTATCTTTCTTGGCTTTTCTTAAAGCATCATTTAACCTGCTAAATTCCAAGTTAAGATCGATTTTATTAAGCAATTCCAAAATTGCTTCAGCCCCTATTCCAACCCTAAAACCAGTGTACTTCTTAATGTAATTAAAAGCATCATTTAAAGAAAAGGGGAGAGAGCTGTTTTTCAAACTTTCATAAAAGATTTTTCCCTTACGGTAATCTTCACTACTCTTACTTTTAATGAGATTTCTGAAGATATAACCTATCACACGACGCATTTTTTGTCGTGTTGAAAGTGAACCCTTACCAGTTAAGTCCAAAACTTCTTTGAACTTAAAAGGCATGATTTTATCATCTTTGATCTTACCTGTATCAAGTACTATGTAGTTAACAAAGTACAAAACCTGTTCAACCTCTTTGTAAGAGATGTTTAAAACTAATGATATTTTGGAAGGAGATGGTAATTCTTTTGACATCCAAATGTGAGCTACAGGACTCACAAGTGCAATATGTCCCATCCTTTCTCTACGTACAATAGATTCAGTAACTCACACCCCACAGCGATCACACCTCACACCACGATATTTAATCTTTTTGAACTTGCCACAAGCACATTCATAGTCCTTAACAGGTCCAAAGATTGCTTCATCAAACAAGCCTCCAGGTTCTGGTTTTAATGATTTATAGTTAATGGTTTCAGCTTTTGTAACTTCCCCTTCAGATCAGTTCAAAATGGTGTCATTGGAAGCGATGGAAAGTTTAATTGCTTTAATGTTTTTATAAAGCTTGTTATTTCTTTTATTACGTCTTGTTGTTGTCATTGTTAATTTCTAATAACCCTCAGTGTCAAATTCAAAATCATTGAAAAATTCATCTTGTTCCCCATCACTTTGCAAGATGGAAACATTATTGGAGTCTTGTTGGGTGTTGTCATCATAGATAAATGAAACAGATAAAGCCAAGCCCTGTAATTCTTTTGTCAATAATTTAAATGATTCAGGGATACCAGGCTCTGGGAAAGCTGCACCTTTAACAATAGCAGCATAAGCCCTATTTCTTCCTTGTACATCATCAGATTTAATGGTTAAAAGTTCTTGCAAGTTGTAAGCAGCTCCATAAGCTTCTAATGCTCACACTTCCATCTCACCAAACCGCTGACCACCATTTTGCGATTTACCACCTAATGGTTGTTGGGTGATCTTAGAATAAGGGCCAACAGCACGAGCATGGATCTTATCATCAACCATGTGATTCAGCTTCATCATGTACATAATTCCAAGTGAAATAGGTCTTTCAAATGGCATTCCACTCCTACCATCAATGAGTTTAAACTTACCCTGATTTTTTTGGGGATCTAATCCTGCTTCTTGCATAACATCTTGGAGATCTAAGAAGTTCACTCCTTGGAAAATAGGGGTTGCAATTTTGTAAATAAGATCATCAAAAGACATCCCTATCTCTTTCAAAACTAAACTAATGTCACTGTTATCGAGTTTTTCAAGTGCTTCTTTTTCACTTTTAATATTGCGATCATTGATTTGGCTTTTTAAACCTTTAATTAATCTTTCAACCCTTGCTTGAGGTTGATTGATTTCAATGGCAAACTCCTTAGCTTTATTTTGATCAAAACAACTACTAATTAAAGAACGAACTGCTAGCTTGTGTGCTGCATAACCCAAGTGGGTTTCAAAAATTTGTCCTATGTTCATCCGACTAGGAACACCAAGGGGGTTGAGTAGAATATCAACTGGGGTTCCATCTTCTAAATGGGGCATATCTTCAATAGGCACCACTTTAGAAATAACTCCTTTATTTCCGTGTCTACCAGCTAATTTATCACCAATTTGAATCTTACGTTTTTGAACCACATAAACCTTGATCATTTCAATCACACCATCATTAAGTTCATCACCATTAGCAATTGAAAAACGTTTTACAGCTGAAACAATACCATCCCCACCATGGGGAACTTTAAGTGAAGAGTCTCTCACGTTTTGAACACTTTCAGGGAAGATGGCTTTAAATAGCTTTTCTTCAGGAGAGACTTCGACTTGACCTTTAGGGGAAACTTTACCAACCAAAACATCCCCTTCTTTAACTTCAGCACCCACCATAATGATGCCATTCTCATCAAGATAGCGTTTGTTTGCATCACTAACATTAGGGATATCACGGGTAATTTGTTCATCACCATTTTTAGTAGACAAACATTGAGCAACATACTCATTAATGGTTAATGAAGTGAGAATATCTTCCTTAACTAATCGTTCTGAAATGACAATTGCATCTTCATAGTTATAACCATTTCAAGTTGTAAAAGCAACTAAAACATTCTGTCCTAATGCCAACTCACTCTTATTAACAGCAGGGCCATCAACAATGATTTCATCCTTATTAACCCTTTGGCCTATTTCAACAATTGGTTTGTGGTTATAACAAGTATTTTGGTTGGAACGTTCAAATTTAACTAGGTTAACTGTCTCTTTTTTAGAACTATCACTTGTAATAATAATCTTACTGTTATCAACATAACTAACAACACCTGAGCAAGGAGAGGACATTGTTAAACCTGAATCACTAGCAATTTTGTGTTCTTGACCAGTACCTACTGCAGGAGCATATGGCTTTATTAAAGGATAGGCCTGACGTTGCATGTTGGTTCCCATTAATGCTCTAGCTGAATCATCATTTTCCAAAAAGGGGATTAAAGAAGAACCAATGGAAACAACTTGGTGTGGTGCTACATCAATGTAATCAATCTTTAAAGGATCATAAAGTCCTTGCATAGATCGATACCTACCAATAATTTCCTTATCTAAGATCTTGTTATCGTTACTAATATTGACAAGTGAAGAGATCTCAGCAATAATATGTTCATCTTCTCTAAGCGCAGTTAAATATTCCACCTCATCAGTAATTACCCCAGCTTTGATTTTGCGATAAGGTGCCATTAAAAATCCGTTTTCATCAATCTTAGCAAAGCTAGCTAAAGACATGATCAACCCTATGTTCATCCCTTCAGGTGTTTCAATAGGGCAAATTCTACCGTACTGAGAATAATGCACATCACGGATATCTAAATTAGGGTCCTCTCTTGATATTCCCCCAGGTCCCATTGCTGAAATTCTTCTTTTATTACTCAATTCTGATAAAGGGTTTTGGTGGTCTAAAAATTGGGTTAATTGGTGGGTATTGAAGAAGTCTTTAATCACAATTTGAATTGGTTTTGAGTTGATTAAAGATTTAATAGTTAATTCCTTTTTTTCACCTTGTTCAATAACCTGACCCTCTTCATTAATTTGCTGGCCACGGTTAACTCCATCAGCAATAGTTAACTTTTCTTTTAAAAAGCGCTCCATTCTAGTAAAGCCGCTTTCTAATTTAGCAGTAATTAATTCATTAATTAACTTAACCCGCTTATTACCAAGGTGATCAATATCATCATATTTACCAATTCCATAAGGTAAATTAATCACATAACTAATTGAAGCGATGAAATCACTGAGAGTCATTGCTTTGTTCAGATCATTTTCGTTATGGATCCCTATAACTGGTACTGAAACACTAAGATTATCATTAGTGATATATACATCTATCGTTTCATAAAAGAGTGATTCTTTTGCTAAATCAACAGCCTTACCATCAGTTGTAAGTTGCATTTTATTAACAAAACTAATTTCATTGTTCTTAGCAGCTTGTTTGATTTTGTCAATCTCTTCTTTTAAAAGTAAAGTACCTTTTTTTAAAAGTAGCTTGCCATCTTTTAAGAACAAATCACATGCCAGAGTTTTTTGATAAATACGTTCTGTTAAACGTAACTTTCTAGATACTTTGTATCTCCCTGCATTAGAGAGGTTGTACCTCTTGTTTCTAAAAAAACGCGCACATAAAAGTGCATGGAAACTCACTTCATGACACTCTTCAGTGTTTTCAATATCACGCAGTGAAATAGAAAGTTCATTGACAATGTGTTTAGCAGCTCTTTCAGTAATAACACTATCCATTTTGTTTTCCATTTCCAGTTCAAGTGCAGTTAAACTAGAATCATTTGGACTTGTTTGTTTTAGCGCATTATATTGTTTCTCAAGCGTTACATATTCATTAACAAGTGATCTTAATTTCTGATCAGAAGCGATCCCTTTTCTTCTTAAATCAGTTTTACCATCTCTAAATTCTTTTAAAAGGTTTTTGATTTCAGGATCAACATTGCTTAAAAAATCCTTAGCATTGTAAATTTCCGCTTCCAATGAACGTTTGATAAATTCATTGTTTTTAAAGATTTTAAGGATCTCACGATTATTTAAACCAAACGCTTTTAAAAGTGTTGTAATAGGGAAAATCTTAGCACCTTCTCTTACCGCATCTCTTAAAAGAATTTGTACAAAAGCATCTTCAATCTTTTTTTTATTGGAGATGTATATGAGCATCACTGAACCATTAGCAGGTAAAACCTCACAAACATAACCTTCCTGTACTCTTTTACGGGAGTTGGATAGTTTTAGTTGGGATTTTGTTAGCATGTATATCCCTGGAGAACGGGTTATTTGGGAAATAACAAACTTTTCTATCCCATTGATAATAAAAACCCCATCATGGGTTATTAAAGGCATGTTAGCTAAAAATACACCATTTGTATTGCTAGCAATATTTTTCTTTGATTTCCGTGCTTTTTTAACTGTTCCTTTATCATTATCAACTAATTCAAGATCAGCATAAATACCAACTTCATAAGTTTTAGATTGAGCACGTGATTGTGCTTCATCACGTTCTGGTTCAGTTCTGTGTAATCCCCTAAAATTGATAGTGTAGCGATCATTAGGGGATTTGATTGGAAAATATGAGGCAATTAATTTTTCAAGATCATGATCTAAGAATTTTTGGTAGCTTTTAATCTGAATATCAGCTAAATTTGGTTGGATAAAATTGGTCTCAATTTTGCCATAATACCTTCTGGTAGCTGTAGGGGAATAACGTTTTTGAAAGAAATTAGATTTTTGTGACATTTGTGGGGTGAAAAGCGGATCAAAACATTAAAAAGCCACTAACAGCGTTATAAAACGCATGCTAATGGACTTAATCACAGTTCGCTAAATTATATTAATTTTTTAACTTGTTCAATAAAAGGCTTAAAATCAAAGTTCTCATGATAACCGTTGACTAAAACTGTTCCTTTAACATTAAGAGCGCTAAAACTTAAAAATTTATCTAGTAATTCTAGAACAGTTTTACCCCCACCCGCAGTTGCTGGAGTTACACAACAAATAACTCCATTAAGACCTTTTAAAAATTGATTTCTACCATACTGCTCATCACGAGTCATCCAGTCGATGATGTTTTTAGCAAAAGCAGGGATAAAACCGTTGTGTTCAGGGGTAACAAAAATTAAATTGCTATGTTCTTTGATTTTTCTAACTAAAGTTTTAATTTTGTCAGGAAAGTTAGTTGCTTCTAAATCAACAGAATAAAAATCAACTTGATAGTCTTTTAACTCAATCAATTCACAACTTAAAGAATGTTTTAATTCATTAGCAAATTTCCTGTTAATGGAATGTTGCGAATTGGAGAGCATTAAAATTAATGACTTTTGATCTTTTGACATAAATTAACACTTAAACTTTAAGTAAAAAGTATTTAAAAACAATTTAATAGAAGCGTATAGCTATTTTTTTCAATGTTGTTAATTATTGAGAAAATAGCAACCTTGATAAGTCTTAATATCAGCAACCTTAGTATCTAAACTAACGGTTGGTAAATTGGGGTTTTGTTCTTTATTTCCTTCAAGTAAACAGATACAATTCTCATCAAAATTAAAACCTTTCATTATTTCAACAATTAAGTGGAATTCCAAATCAAAAATCAAAGCATCAACTTTTAATTTAGTAGGAGAATTTGAAAATACTTGTAATTTGGAATAATGCAGTAAAAACTGCTGATTAAAATCTTTAACAGTTTGTTGTCAAATTAAGTTCTTTTGCTCAAAGTTAGGATGCTTAATGTTTTCTAAATTTTTTAACAAGTATTTATGAGGCAAGATAAAATGGCTGGTGATTTTTAACTTTTTTTCAATTGTGAATCTGTCTTGAAAACTAGTGAAACTAAAATCAATGTTACTTTTTTCAAAGTTAGTTTCATAACCAAACACACCGTTTAGTTCAACAAACATTACGTTATTTTTAGACTTGTGCTTTTTTTCTAATTTTCTAACTAACTTAAAAAACTTATTTTGAAATGATCTTAAAGAATAAACTTCATGTTTTATTTTTTGTTTTTTAATTTCTTTAATTAGCGAATCAGAAAGCTTAATAGTTGTTTTTTCTTCTGGTGAATTAATTAATAAAAAAACTATTTTCAAACCCTTAAAAGTTTTCAGTATTTTTAAAAACTTATTAATTTCTGTATCTGTATTTTTGGTTTTTTCAACAAAAGCAAATACAGTATCGTTTTGACTTTTATTTCTTAATGCTAAACCAAATGCACTTCACAAGTTATTATCAAAACCTAAAAAAGGGAAGTTATCAAAATAAATCTTTTGATATATTGGAAGACTTACTTTGTTAACAAGCAGATAATTTACTTTTTTAGATCTAACTAAAAAATTAACAAAATCTAGATATTGTAATGTTGATAAAAGTCCAACCTTATTGCTATTTTGAAAAAAATCATAAGCTAATAGGCGTTGCTCATTAACGAGGGTTTGATCATTAATTTTATGAGACATTGTTTAATAAATTTTACTTAGTTTATTATGATTCAGGATATCATCCATTGCTTTACAAAAGCTTTCATATTTACTATTAAACATTTGGTGTTCACTGTTTAAGATAACAGATGATTGCAAATTATTGTTCTTAATTAGGTATTTATTGAAATAGCTTAATGTTAATTTTGTTGGCATAAATAAATCATTTTGAGCTAAAACCAAAAACACTGGTAACTTTAAATTTTTATAAGTCTTATTTAAAACTGGTAGTTCTTTCAAAATATTATTAGAAAGCTTTTTAGCCATTTTACTGTGTTTTGACAGCTCATTTTCTCAGCTGTTTTCATCAGTTAAATTACTAAATTTAGTTTGAAACATTGCTTTAAAATTAGCCATTCTTTCTGCCTTATCTTTTTTAAAAGAACTTTTGATTTTGAAATATCTCAATAAGTTACAGTATGAAAGAGGTGCTAAAAGTATGAGTTTTTCAACACGTTTAGCATTTAATAAATTCATGGCATAACTACAAACAGCAGCACCCATACTGTGTCCTATTAGAACGATGTTTTCCAGATCCTTATTTTCTATAAAAGCAGCTAATAGTTCTCCATAATGGATTGGAGATAATTCCTTATAATCAAAACCATTTACATTATGACCAGGTCAAAGTGGAGCGTAATAGTCATAATCAGTAAGGGTTTTATAAAAACTTTGAAAGTAGCTATGAAAAACAGAAAATCCATGTGCAAAAACAACCGTTTGTTTTTTGTTTTTTTTGCTGGCTGAAAGAAAACTTCAAGCTTAGAAATATCAATCTGATTAAGATCTGAAAACCTTTTCACAATTATTTTTTTAACACACTTTAAAACAACGTGAGCACAAATTGTGATCTAATTTTTGCTTAATGATTGTTTGAAAATTTCAGCATCTTTCACACATTGTTTTTTTAGTTTTTTAACTTTAATTTCATTTTCATTATTTGTTAAATTAACACTGTTTACATTAAGTCAAAGTGCGAGATTATCCTTTAATTTTTTGGGTATTTTTTTATTAATTCCAACGGTTAATTCAATTTGATTATTTTTAGAAATCAACCCTTCTTTTCTTAGCTTTTCAATTTCTTTGAAAGCAGCATTTTTAATACTAGTAAAAGTTTTATAGATATTTTCTAAATTCTTAGAGTTAGCAACTTTAAAAACAGTCGGTTTTGTAAAGAGGTTCACACTTATTGGTTTTTTATTGAATGAATAGTTTTTTCAAGCATCTTCTGCAGTGTGGGGAATAAAGATATTTAAAAAACTGATTAGTTGTGTAAAAATATAGTTTAAAACAGCTTGTTTAGCTAAACGATTAGGATTATTTTTAGCATCACAATATAAGGTGTCTTTAATTATTTCAAAGTATCAGCTTGATAGTCATAAAACAAACTTATTAATCACTTTTAGGCAACCTAAAAAATTATATTTCTCTAAAAATTTCTCAATTTGTTCTACTAGTGAATTAGTTTTATGGATAACTATTTTGTCTTCTAGTGAAAACTTATAATCATCCATTGATGTAAAGTTAAATCCATTAATATTACCCAAAATAAAACGTAGTAAACTATTTCTAATTCTGCGGTATTGTTCAGCAACTTGTTTAAGAATATTAACACCTATTTTGTTATCAATTTGTCAATCAGTATTAGCAACTCACAACCTTAATATATCCGCTCCATATTGATCACAAATTTTTAAAGGATCAACTATGTTTCCTAATGACTTTGACATTTTATTGCCATTTTCATCAAGTGTAAAACCATGTGAAACAAGTGATTTAAAAGGGATTAAATCATTTTGAATAATTCCGCAATTTGAAGAAGAGTTGAACCAACCCCGATATTGATCAGAACCTTCAATATAAAGATCAGCTATTGAACCATATTTATTTATTTCCAAAACATTATAGGAAGAACCTGAGTCAAATCAAACATCTAATGTATCAATCTCCTTGTGATACTTAACTCATTTTTTGGTTTTATCAGGTTTTAAAAAACAAGTTACATCTTTTTCAAATCAACTATCAATACCATGCTTTTTCAATTGTTTAATTGTGTATTGAATTGTTGAAAAATCTAACAATGGTTTGTTATTTGCATAAACAATTGGTATAGGCAAGCCCCATACTCTTTGACGTGAGATACATCATTCATCACGTTGTAAAAGCATCTCTTTTAATCTCAATTGATTTTTTGAATTTAAAAAATTAACTTGATTAATTTGTTTTTTTAACTGCTTTTTTATTGATTTAGTTTTAATGAATAATTGTTTGGAAGCACGGTATATAACTGGAGTTTTTGAGCGTCAATCATGTGGTTCGCGGTGGGAAATAACTTCAGAAAAAATAAAGCTATTGTTTTGTTTTAAACGATTAATAATTAGATCATTTGCTTTAAGATAAAAACAATTCTCAAGTTCTTTATCATTAAGTAAGTTATTAAATACACCTTTCTCATCAATAGAAATCAAAACTTCTTTAATCTTGTTTTTTTGACAAAGATAAAAATCATCAATTCCAAATGCAGGGGAGCTGTGAACAATACCTGTTCCCTCATTATCAACAACATGTATTCCCATTAGAACTGGTAAAACCTTGTTATAAAAACAATGAGAATAGCTTGAATTTTTTAAATTTGAACCCTTGAATTTTTTTAGTTTAATTGCATTTGTTCAATTTAACTTATTTGTAAAAACTTCAAATAATTTTTCCAAGATAACAAATTTTTGTTGGTTATATTCAAAAAGAAGATAATCAAAATCAGGATGAATGGCAATTGCTTGATTAGTTGGTAGTGTTCAAGGAGTTGTTGTTCAAACTAATAAATTAGCATTTTCATCTAAAAAATCACTTTTAGAAACTTTAAAAGTTAAATAAAGTGCAATGGAATTAACTTCTTTATATTCAATTTCCGCTTCAGCAAGTGAAGTTCTTGAAATTGGTGATCAATAAGTTGGTTTTAAATCTTGAAAAATGAGCCCTTTTTTAATTGCTTGTAAAAATAGTTCAAGTTCCTTAAATTGAAAACTCTCATCTATTGTGTAATAACAGTTTTGAAAATCATTTAAAAGTCCCAGTCTTTGAAATTGTTCTTTTTGAACTGCAATTTGTGAAAGTGCAAACTGATGACATAATTTTCTTTTTTCAACAGTTGAAAGATTGCTATAACTACTAGGGTTTTTCTTACTAACTGCATGTTCTATTGGTAGTCCATGACAATCCCAACCAGGAATAAAAACAACATCATATCCTTCATATAACCAACTACGTAAAATGAAGTCTTTTAAAATCTTGTTAAGAGCATGTCCCACATGAATACTACCATTTGCATAAGGTGGTCCATCATGCAGTATTTTTATCTGTTTTCCTTTATTCTGTTTTTTTAATTTTTGAAAGACTTTTTTATCTTTTCAAAAATCATGAAAATTCTTTTCACTAGTAGATAAATTTGCCTGCATCGCAAAGGATGTTTTAGGCATTAACAATGTCTTTTTTAAGTCCATTAAATCAGAGTAATTTTATTAATATTTATCTAACTTACATCTGATGTATAAATCAGTAATAAACATAGTTTTATTTTGTCCAGAAATTCCTAATAACACTGGCAACATCGTACGTAGTTGCACTGCTTTTAAAGCTAATCTACACTTAATTAAACCTTATGGCTTTTTCTTAAATGATAAAAGGATGGTTAGAGCTGGTTTAAATTGTTGAGATAAAATTCAATTATTTGAACACAAATCATGAGAACATTTCTTACAAGCAACCACTGAAAATAAAACTATTTGGCTTTTAACTAAAAGTGGTGATAAAACTCCTGATCAAATTTGCATGACAAATAAATTACCAAACGAACTTTACTTTGTTTTTGGTCAGGAAACAAAGGGATTACCTAAAACAATCATGGATAACTTTAAACAAAACCAAATTAGAATTCCCATTTGAAATAGTGTTAGAAGTATTAATCTTGCTAATGCAGTTGTCTGTATTTTGTATGAATATTCAAAGCAAAATCAATACTCTAATTTAGATAAACAGTGCGCTTAAGAAAAGTTAAAAACGCTCTTTTAAAAATTAATCAAAGTCCTTATTTTTATTCAAAAGATAAGTTTGCTAAGTTTACTAAAAAACAATTAGTGCTGGAATTGGGTTGTGGTAAGGGTACTTTTTTAATCAAAGAAGCACAAAAAAATAACAATTTTCTTTTTATAGGAATTGAACGTGAACCTACAATTGTTTTAAAAGCAATTAACAAAATTAACAAGTTGGATTTTAATTTGGAAAATATCTTATTGTTGTGTACAGATGCAAAACAACTTGATGATTATTTTCAAGCTGAATCTGTTCAAAAAATCTTTATTAATTTCCCTGATCCTTGACCTAAAAAGCGTCATATACAAAGACGTCTAACAAGTCCAGATTTTTTGAAACTTTTTTGAAATTTACTAGTAAAAAATGGCTTAATTGAGTTTAAGACTGATAATGATAAGTTATTTGAATATACTTTAACAACATTGCAAGAAAATAGTCAAATTTTTGAAATTATCCATCAAATAACTGATCTTAACAATTCTGAATTCAGTTTTCAAAATAGTATTACTGAATATGAACAGCGCTTTATGGAATTAGAAATTCCAATTAAAAAACTAGTGATTAAGAAAATAATTTAATTAAAGTTATTAGAATAATTATTAGCTTGATGGCGATTGTGGCGAAGTGGTTAACGCACCTGATTGTGGATCAGGCATTCGTGGGTTCAATTCCCATCAGTCGCCCCATTAAAGATTCAAAGAGCAGCAGTTAGCTGCTTTTTTTTATTAGCTAGTTTAGTGGAGTATAAACACTATCCAAGAAATTTAAAAAATCAACACTAACTCTTACTGCGTGTTGTTTAATTCCATAAGATTTTTGTGATCTTTGAACTGCTGGTTGAGAAGATTGTGGTGAAGTAGGAGTAGTATTTTGTCCATTACCACTACTTGAGTCACCACCTGAACTGCCATCACTACCGCCCCCTTCACCACTTGAAGCTGGTGGGGTTGAATCACCCTGACCTGATCCAGGTGCATTAGGATTTGTAGAGTTATCCAAACCATAAAAATTAGCTCTTATCTTATTAAGGTTATCTTTTCTAAAAGCAATCACAATTGCTTTAGTTACATCATTACCAACTGTTTGAGCATGCACATTTGATTGATTGATTAAGTTAGCTACATTACCACCATAAGTACGTTGAATAAAATCAACTATTTCTCTATAGCGAACTGCAGAAGCATCTGATCTTCTTAACTTATTGGAATTGTCTTTATCTTCTTGACTTGCAAGTTCGTTTTTTCTATCCACAACAGGAAAACGTGCATATGGGTTTCACTTTACTGAACCAGGAGCAATATCATTCCAAATTAAAAATTTAACTCCGTTTGGATAAGTAGAATAACGGTTTTGCACCTGTCTGATGGTGGTACCCAAGGTACCTCCTCAACTAAGAGAACTTGTACCTTGATTAGTAGAACCGTTTAGAAAATTAATTTGGGAACTATCTGTTTCGGTTGCAATAACAACCACATAATTACCATCGTTATATGATCTGGTTCCAAAAACTAAGTTTCTAAATGTTGTACTGTTAACAGGCAATTGGTTGTAAGCTGTATTTTTTTCCACACTTTCAACAGAAAGTTCACGAAAAAAAGACTGATCGATGTTACTACAAGAACTTAAAATCATTCCTGTTCCTAGTAATAAAACTAACCATGCTTTTTTATGTTTTAGATTTCAAAAAAGACGCTTCATTTCTAAATTGACTGGTGTCGGAGACCAGACTTGAACTGGCACAGTCTTTAACGACCACAAGCACCTCAAGCTTGCGTGTCTACCATTTCACCACTCCGACTATTTGCTTATAGTTAATATTGTTTATATTAATAAAAATTTTAATACAAGAGTTAACAAGGCTATTAGCTTGTACTAGCTTAAACTAGGAATATAAAGGTGTTATTTCAAGCTAAATTCCCGCTTTTTTCTCCTTAAATTCAACTTTTTTTATCCGATTATTGGTAAATAAATCAGTTTAAAAAGTTATTAACAAAATGTTTATAAGTCTCAAAAAAGGGTTGTTTTAGACTGTTTTTAATGTCAATAAGTTGTAAATAAATGGCAGAAAAATCTTTTTTTAGCCTTTTAATGCAAAGTAATTTCAATAAATGGAGGATAGCGTCTTATACTCTTTTTACTTTTGAACGTATATGCAACCCAACTATTACCGTGTGATCAAAAAAGCAACATCATTTTCTGGATTAGAAATGATCTCTCATGCTTATGGTCAAATTGCGGGAGTGGAAGTTGTTGGGTTTTATTTATGACTAATAACAGAAGCTAATATTCAGGCGTTCAACAGTGAAATTAGAACCCCCATTTCACGATTACAAAATGCCTTTAATCCTTTTGAAATTAAAAAAAACCAAGTCCCTGAATGGATTTATAAAACTATTAGCAAATTAGAGAGTCTTGGTTTAGTGAGAACTTTTTTTTCACCAAAACGCTCTGAAATAACTTTTTGTATTATTGATCCTTTAGATTGGAAAGAATTTAAGCAAAACAAACAATTAAAAGAAAAACTAGTTGAGGCAATGGGAAAAGTTGAATATGACCGAAACTGCTTAGCTTTTGATCAAATCGACAATCTTCAATTTGATAATGCGCTTGAAATCTCTGCTAACTTTGAAGTTAATTTCACTGCAAACCAAAGCGATGTTTGATTTAGCTTTAACTTCGAGGAACTACATAAAGAACTTGTAAAAAACAAACTTTTAATTTCTTTAGATGAAAAAGCTAAGACTTTAATTAATGGTTATTTTGAAAAATACAAGCTTTCATTGCAACAAATTACTGATTGCATCATCAACAGTACTCAAGAGAATGAGCTTGATTTTCAAAAGTTAGAAATGATGTTTTTTCAAATAGTAAAAAATGATACAGCTCCCATTTTAGAAACAGTTTCAAACAACAAAGACTTTTTTTATAAGAATGAAATTTTAGATGAATCAACAAAAAAAGCAATAACAGACTGTCATGTAAACTTCAATTCTGAAAAATACCTTTTTCTTCTATATGGAAAGATAGATGAATCGCAATTGCAATTAGTTAGACAATTGAGAAGTGATTATCAACTATTAGATAAAGTAATTAATTTAGTATTAGACTTTTCCTTTTGAAAAAATAACGGAATGTGAAGAGAAAAATACATTCTAAAAATTGCCCAATCCATCAAAATTAATAACAGTCAAAACAGTTATGAAAAAACCCTTAATAATTTTATTAGAGCACTAACTTTAAATAAAAAGCATTCTTTAAATAATATAAAACCAGTTGAAAAAACAATTTCCTTTACTGAGTATTTTGAATTTATTAAATAATGAAAATTTCACCAGATCAAAAAGATAAAAAGTCATTATTTAAAAAACATTTTGCTTATTTGAAATTAACTGAAAAAGAACTTAATGATCCTAAGATACAAACACTTTTAGAAGTTGCGTGGAATCATTTTGATCAGTGTAGAAAAATTAAAGAAAGAAGATGTAGTAATAAAGGTGATATCCATCTTCAAGCTGTTAGAGAGTGGTTACCTTGAGAATTTAACGCTAATCAACTTAAAAAAAATAGTACAAAAGAAACAACGCAAGTTAGTGAAAATGAATTTATGGGTAATGTTATGCTCATGCAAACTATTTGTCCTAAATTAGTTAATAAAGCAAACTGATTTGATTTAACTTATGAGCGTTTTATTGTTACAAAACCAAACTGATTTAAATACATGGATTTGATTCCAATGATCCAAAATTTACCAGTAACTCCAAGTGATAAAAATAGTTTTGGTTATGCATACAAAAAGATATCTAATCTTTTTGAAACTGAAAAGAAAACAAAGAAAAGGATATTTTTTTTCAATTTACAAAAGAATAACATTAACAATATGGCAGCTTGTATGTTAACTTGTGAAATAGCTAAGAAAAATATAAAAGTTGCTTTAATTTATTGTGAAGAATTTGTTAGCAGATATGACAAAAGTTATTGAAAGGTAGATGATGATTTAAATTTACTTGATGAGGCAAAAGTAATCATATTCATTGGTTTAGGTCAGGAAAGTTTTCATAACAAAAACTATATCTTGTTTATGACAAGGCTTTTTATAAATTGTTTTTTGAAAAGAAAAGATGTTTTCTTTTTTAGCACTACTTATAGTGATGGTAATGGATTAATTCAAACATTTAAAAACCAAATTATTAGTAGTGCAAATTGAGTAAAACACTTTTTTGAACAACTTAATGATTTATTAATGATTAATATTTAAAAATTACATTCTTTCACCAATAAATAGCTAAGTTTATGTAAAATTTATCTTGTATTACCTGGAGTGGCGGAATGGTAGACGCGGTGGACTCAAAACCCACTAGGAAACTGTAGGAGTTCAAGTCTCCTCTCCAGGACCATAATTAAATACTTAAATTTACACTACGAAATTACAAGCTCAGTTTAAAGCTGAGCTTGTTTTATTTTTATAGTGATTTCTATTTAGCTAATGCTCTTTTTCAATAAAACTTTTGAAAGGAACGCATAATTGGCACTCCAAAACAACTAACAAGTCCAAATTTCACCAAATTAAAAAACGAATATAAACAGAAAATTCCCAGCCAATAGTTATTAATTCCAAAGAAAAAGAAATGAAAATCAGTGTTCTTGTTATATTGTTCAGCAACTATTAAAAAGTTAGGTGTTTGAACATAACCAAAGTAGTATCAAAACAAAGGTGTTATCAAAATACCGTTAAGCGTTGTTAGTAATAAAGCATTGGATAAGGTTGTAAATAAAAACACAAAGATTCAACAAAATCTTCTTTTTGAAAATAAACAAAAATAAAGCCTTGTAAATAAGATAGTCGATAAGTTAGCTAAAGTTACAGTTAGAACACCAATAACATTATTAGTATTTCAAAAAAAACTAGTTAAAGCAGAAGCTAATGTAATTGTTAAGCTTCAACCTAGTGACACAAAAAAAGCACATGCAACTAAAAAAACAACGGAAATATCAAAATTTAGTTTTAGTTGTGAGAAAAAAGGAATACTAATAAATTCACTAATTAATGATGTAATGATGGCCAAAGCAAGCATCATTGCACTAAAAGTAATATTAAATAACTGATGGTATGATTTTTTTGGCAAAAAAACCAGTTTATTACCATTCATTATTTAAAAGACTCTCGAATTATTAGTTAATAATAATATTTATTGATATGGACAAATTTTTAATTGATGTTATTGTAGAAATCCCTAAACACAGCAAAATAAAGTATGAGTATGATCGTCAAACTGGTCAAATTCGCGTTGATAGAATCCTATTTGGAAGTGAATCATATCCACAAAACTACGGTTTTATTAAAAATACATTAGATTGAGATGGGGATGAACTTGATTGTTTTATCTTTGCAGATCAACCATTTTTGCCTGCAACAGTTGTGCCTACAAGAATTGTAGGAGCACTTGAGATGATTGATGATGGGGAAATTGATACTAAGTTATTAGGAGTTATTGATTGTGACCCTAGATATAAAGAAATTAATCAAATTAGTGATTTACCTAAACATAGAATAGAAGAAATTCTTATCTTTTTAAAAACTTATAAATTACTTCAAAAAAAGACTGTAATTATTAAGGGTTTAAAAGATGTTTGTTGAGCTAAAAAAGAATATGAAATTTGTTTGCAATTAATGAAAGATTATGGTCATTTATCAAAAGATCAATTTATCCAAAAAATGCAAATTCTTCATCCAGAACATTACCAAAAGTAAATATGATAAATGCCAATCGCGGAATGCTTTTAGAGACAATTGTTAATCAAACAATTGCTAGGTTAAAGGATCATCCAGATATTTGACTGGAAAAGCGGTTCTTACCTATTAAGCCTATAGCTTTTCGTCATGCTCATGTATCTGGCAACGTATCACAAAAATCAAAAACTGATTATTATGGAATTTACAAGGGAATGTATTTTGATTTTGAAGCAAAACAAACAAATAAAAGTAATTTTCCAATTGCTCAAATAGCAGAACACCAATTAAATCATTTGAAAAGAATTGACCAAATTGGAGGAGTTAGCTTCTTGCTAATTTACTTTCAAACTAAAGATCAAATTTTTGCATTTCACACTAAGGATCTTCTAGAAACAATAAAAAATCAAGAAAGCAAAACAATTAAAAGGGAATTGATAGAACAAAAATCTCAGAAAGTTCCACTATTGTATCCTGGTATTATTGATTTAATTTCAATAATTCAAAGCTTTAAAAATTATTAGTCTGCGTACTTTCAACGCACTTTAGCTGCAGTTTCTTTGATCGTTTTGCTTGGACTGTAGCGCACTAATGGTTTTGGTGGGATATGTCTAATCTCACCCGTTTGCATATCCTTCTGATAACGTGCATTTCTAATAGTAATCCTTAATTTACCTAAATTTTCAGGTAATATACAAACACTTCTACTTACCAATTCATTTAGTAACAATGTGTTAAGGTATTTAAAGATTTCCTTAATTTTTTTTTCTTTTATACCAGTAGCAACTGCAATTATTTTATTGATTTCACTACGAGAAAGTGGCTTACTTGTATTTGATGTTTTTTCCATATAATTATTTTTTAAAACTTTATTAGCTCTGTTATTGCTAAAAACAAAAGAGAAACAATTTAACGCATTCTATTTTATTATTGATTCAACAATTAAATTAAAACACTTATAAAGTAAATTTTTAGACAATTATTTCTATTCTTAAGACATGGAACAAAACAATATTAAAGAACAACTTATTTCTTTCTTTAATCAAGCATGTTCTACCCACCAAGAAAGACTTGATTTTATCTGTTCTACAAGAGAAAGTGATACTTTTTCTAGTGTTGATGTACCACTTGAACCCATTAAAAATATTATTGAAATAACTAAAGATGAAAATCAACAAATTGAAATTACAAAAATAGCTGTTAATAACATTAAAACATTATCTTCTGTTGGTGCAACCGGTCAGTATATGGCATCTTTCTTTTCAACAAATAGTGAGCCAGCTATAATATTTTGCGTCATTTATTTTTTATATCACTTTGGTTTTTTAAAAGATAACAATAAAAAACAAATAATAAAAAAGGCTTATGAAACTATTGCTGATAACATTGCTGACTATTTAAATGAAAATTAGGTTTAAGACTTATTATTTTGTGTAATGGTAATATTGTCTTTAACAACTGCAATGTTGTATCCAACACCTTTTTTTATCTTTCCCTGAAGGATATATTTAGCTATTAAAGTTGCAACACTATGATCAATAAAGCGCTTAATTGGTCTTGCACCAAACTGTTGATCAAAACTACTTTTATAGATAAACTCTGTTAGATTTGAATCAAAATTAAAAAATAAATTTTGTTTATTCAAGCGTTTTGAAAGTTGTGCCAACAAGCTGTTGATTATCGATAAAACTGTATCTTTCTCAAGAACATTGAAAAATACTATCTCATCAATACGATTTATAAATTCAGGACGGAAATGTTTCTTTAGACTTTGAATGGCCAAATCTTTTTTTCCTTCTAAAAGAAAATTTGAACCTAGGTTAGAAGTCATAATTATCAAAGTATTTTTGAAATTAACAACCCTTCCTTGTGAATCTTTTAAAGTACCATCATCTAAAACTTGTAATAAAACATTAGTTACATCAGGATGTGCCTTTTCAATTTCATCAAATAACAAGACGCTATAAGGTTTTCTTCTAACCGCTTCAGTTAGCAAACCTGATTGTTCATAACCTATGTACCCTGGGGGTGCACCAATTAATTTAGCTACTGAATGTTTTTCCATATATTCACTCATATCAAAACGAATCAGAGCTTTTTCATTGTCAAAAAGAACTTCTGCTAATGATTTGGCAAGTTCAGTTTTACCAACACCAGTAGAACCTAAAAAGATGAAAGAACCAATAGGTTTGTTTGGGTCATTTATATTTACTCTACCTCTAATTACAGTGTTAACAACAGCATCGATGGCTTCATCTTGTCCTTTAACTCTTTTTTTGATTTCATCACCTAAGTGCAAAAGTTTATCCTTTTCACTTTCTAATAGTTTTTTAAGTGGAATTCCTGTTGTTTGTGAAATAACTTCAGCAATTTCATTTTCAGAAACTTCAGTTTTAAATAAATCGTGCTTAGAAGTTGCATATTTTTGTTGTGCACTTTCCAGTTCTTTTTTAAGTCTTGGGATATCAGAGTACAGAATTTTAGATGCACTTTCATAATTTCCTTCACTTTGGTATGTCTCTAGTTTGGTTTGAAATTCTTCAATCTCTTTTTTGAGCTTATTAATGTTTTCAAAATCAGCCTTTTCCTTTTTTCATTCATTTATAAGTGAATCACGCTTTTGTTTAAGAGCATCTAATTGTTTTTTAGTTTCTCTAAATATTCTTTCTTTGATTGTTTGTTATCATTTTCCTTATCTTGTTTAAGAGCTGCATACTCTGTCTCAAGATTGATTATTTCACGTTTAAGACTATCTATTGCAACTGGTTCAGATGACATTTCTGTTTTGATCTTAGCAGCAGCCTCATCAATAAGATCAATGGCTTTATCAGGTAAATTACGTTCATTAATATAACGAGTTGACATTTCAACAGCAGCTACTAAAGCACTATCAAAAATAGTGATGTTATGAAAGAGTTCTCAACGTGTTTTTAAACCACGCATAATTGTTAGTGCCTCTTGACTACTAGGCTCGTTAATAAGAATTTTTTGAAATCTACGTTCTAAAGCGCCATCTTTTTCAATGTATTCCCGGTATTCTTTTAGAGTAGTAGCGCCAATTACTTTTATTTCTCCTCGAGCTAGCATCGGCTTTAATATATTGGCAATATCCATTGCACCACTGCTAGAATTACGTCCTAATCCAACTATTTGGTGAATTTCATCAATAAAAAGAATAATCTTGCCATTTGATTCTTTTACTTGCTTAAGAATGGTATTAATTCTTTTTTCAAATTCACCTTGGAATTTAGTGCCAGCAATTAATCCAGAAAGAGATAGTTCATAAATTTCTACATCCCTTAAATTTAAAGGTACATCATTACTAACAACTCTTCTAACAAAACCTTCTACTATTGCGGTTTTACCAACTCCAGGTTCACCAATTAAAACAGGATTGTTTTTGCTTTTACGACTTAATATCTCAATTAAACGACGAATTTCGTTATCTCTTCCAATAATAGGATCGACCTTATTTTTTAATACTTCATCGTTAATATTACGACCAATTTCTTGCAAAAAATTACGATTTTCACCAGCAGGTGTGAAATTAATATTCATTTTAATTGCTCACTCCTTTTAATGCAAGAATTATATAAAATTAGCACTCAAGGCATTCGAGTGCTAATTAAAAACTAGGTTTTTTGTTTATCTCTAATTTATCTAATCAGAGTTGATATTTTTGTCAAAATAATTAGAAACCTAAAATAACTATTAACTATTTTTTTGTGCTTGTTTTCAAATTGAGGGAAATAATGCTTTTAAAAAGTATTCTAATCGTTTTTCAACTCATTTTCGATAGTTTAAAATTGCTTCTGTTTGGGGCATAATCTCAGTTCATTGAAAAGCAAATAATAAAACATAAATAACTGTTTCAAAAACAAGCTTACGCTTAAGTCCACCATAAATATCAATAAGTTTATTTGCTGTTTCTATGGTTAGTTCTTCACGAATAATATTAGCTATTTCATAATAAGGATTATCAACACAAGATCATTCAAAATCAATAAGGATTACTTGCTTTTTAGGTGTTCAAACCAAATTATCAAAAGTAGCATCATGATGACAGAGAGTTTTTGGTATGTCAATATGCTTCTTAGTTAAATTAAGATATAACCGTTTATAAAAAGCGGAAAGCTTAGTTTTTTCAAAATAAACCAAAGGATCAAATATTAAAAGTTCATTAGCAAATGGTTTTCAGTCAACTGAATGAACTTTTTTTAAAGATTCAACCAATCTGCATAAAAATAGATCAGTTGCTCTTTTAGGTTGAGTGCCTTCTATCCACCTTTTTATAGCATTACCTGTTGTTACATCAAAATAAACAAATGGGTTTGAAAAAAAAGGTTCTACTAGTTGCAAAATAATTTGTTCATTTCTTCTGTCTAAAATAACATCTTTTCTTGAAAGTCTTATTTGAAATTTACTATGATCTTTCAATTCACAAAAAAAAGAAAAATTAGTAAAACCAGAATGAATTTGCTCTATCTTGAAAATATCTGTTTCTACAACTTGAAGTTTTTCACAAAGAAAAGCAACCGCTTTTTTTAAATCCATTAATTTTGATGATTATTTTTTAATGTTTGTACTTACTCTCAAAGCATCTTCTACAATCATCAATTCCTCATTTGTACGAACTCTAAAAATTTGATACTTGCTTTCATTTGTTGAAATTAGAGAACTATCTTGATAATTTCCAAATAAATTACTGTTAGTTTTAAAACCAAGTGAAGCAACTTTTTCAATTATTAATTGCACACAATAACTAGCATTTTCACCAACTCCCCCAGTAAATACCAAGCTATCAATTTCACCTGAAAGTTGATTTAGGTATTTAGCAATATAGTCAGCAACACGATTAACATACATTTTTATAGCAATATCATTAATTTCTGGTTTATCAAAAATATCACGCATGTCAGAACTACCTGTTATAGCAAACATTCCACTCTTTTTATTTAATTCATTTACAACATCATTACATGAAAGCTTTTGCTGTTCAGCAATGTAACTAACAATGGCAGGATCAATATCACCACTACGTGTTCCCATTATTAATCCTTCAAGGGGAGTGAATCCCATCGATGTGTTTAGTGATTTGCCTTGTTTAATCGCACAAACACTTGCACCATTACCAAGATGACAAACAATTAAATTAAGTGGTTTTTTATTAAACTTTTTTCTAAAAACTCGTTAATGTATTTATAAGAAGTTCCATGAAAACCATATCTTCTTACTAAGTTATTTTTCTCTCAATTTTCAGGAACTGCATAAAGATAATTTTCCCTTGGAATAGTAGTGTGAAAAGTGGTATCAAATACAGCAACATTCTTAGCAGTTTTTATCTCTTTTAGAAAAATTTCAATAACATCTGCTTCTGGTTTATTATGAAGCGGTGCTAACTTAATGAATTCTTTTATTTTTGCTAGTGAATGTGTATCAACAAGAACTGCATCTGTAAAATAATTTGCTCCTTGTACTACTCTATGCCCTATTAGCCCAATTTCTGAAAGTTCAGTAATAATTTTGTTTTTTTTAAGCGCATTTAAAAAATGCTTAACAGCAAGATTATGATCATTAAATTGAACCTTTTCTTCTATCTTTTTTTGATTAAATTCAAGCTTAAAAAAACCATCAATGAAAATACGTTCACAAAGTCCTTTAGCTAGTACTTGTTTTTTATCATTAAAAAGTTGAAATTTAATTGAACTGCTACCTGCATTAACAACCAAGATTTTGTGACTTTGCATTTAACCGAAGAATATTTACCTTATTTATTAAAAAATAATACTAAATTAATATAGTGTGGAATAATTAAATGTTTTAACTAAAAAGAAATATTATTAAATCTTAAACAATGAAAATTAAAAAATGCTATATTATCGCTGTTATTTCAGCTTTTATTGGTTTTCTTTTTTACTTTTTATCTTCACTTATAGGGATAACAGATGTTAATAATGCAAAAACAGTAGCAACTGTATTTTTTGTATTAGCAACATTAATAGTTGTTAATGCTATTGTCTTCTGGATTCTTTATCTATTCAAAAAAAAGAACGTAGTTAAGTAAATTTATTTGTCACTAATAGTTTTTTAGCACATTAATTTGATAGAAAACAAATTGAATGTAATATGGAAACTATCAGAGAAACACTTTCTTCTATACAAAGAAGCAATTTTTTATTAAATGAAAGCGCACAAATTCTCAATACGTGTGTAAACAAGAACACATTGCTTGACGATAAGCTGCGCAATTCGATTAATTTTGCACTTAGCATCATTAAGCTAGCAGACTTTTGTCTAAAGGATGATACTGAAAAAATTAATCAATTCTTAGGTAAAACATATGATTTACCATGAGAGTTTCAATTTAACCAAAAAGACAAGCATATAGAATGCACTGTCAAAGACAATTTTGGTCGTGAGAAACTAATAAATTTGATTTTTCAAATTGGTGATGCTATTGAAACTTATCATACTACTTTAATAAGATTCAAAATTCCCAAGCATTGTTTGAATGCAAGGGATCAAATCAAAAAAATAATGGAGGGCAAATAAAAATGATTACATCTATCTTTGGAAAAGTTACTTTTGTAGGCAAAAGAAAAATAATTGTTGAGCACAACTGGATTTCATATTGATTTAATACAAAAGAAAACCATAAATTTGAAAAAAATTTGGAAAAAAATAAGCAAATTTTTTGTCATATTATTAAAAAAATTGTCGCTAACCAAATTATAGAAGAGGCTTTTGCCTTTAATACTCTAGAAGAAAAAGAGTGGTTCTGTAGATTAATAGAACTCAATGGTATTGGTAGTAAAACTGCACTTAATTTGCTCAATAATGACCTTGAGGAAATTAAACAATACATTCTGGAAAATAACTACAGTGCATTATGTGGTATTAACGGTGTAAATAACAAAATAGCTCGTGCACTTTTATCACTTGAAATATTTGAAAAATCTGAAAATAATAAAAATATTAAAGGAGTTCAAGTTGCTGATGGTTATGATGAATTGTTTGAAACACTAAAGTCACTTGGTTACAAACAACAAGAAATTCAGGATGCACTAAAAATGATAGAAGTAAAACCTGATTTTGATATAAGTCAGTTAGTTGCAGAAGTAATTAAATTAATGTCTTTTAAGAATAATGAAATTACAAATAAAACCGCCTAATACCTTTGATGAATTTGTAGGAAAACAAGAAATAATTAGTCAAATTCAATTAAGTATTAAAGCATCTAAATTAAATAAAACACAACTAGATCATATCTTGTTATATGGCCCACCTGGTGTGGGTAAAACTACTTTAGCCAGATTAATAGCAAATGAATTGAAAACAAAGTTGCAAATTATTCAAGGTGGACATTTACAAAAACCAAGCGATTTCTTAAACGCAATTTCACTCATTAAAAAAGGTGATGTTCTTTTTATAGATGAGATCCATGCCGTAGCACCTAATGTCATGGAACTAATGTATCCAGTTATGGATGTGTTCAAAATACAAGTATTAATTGGCAAGGATTTTAATTCCAAGATAGTTGAAATGAAGGTAAATCCTTTTACTCTAATTGGTGCAACTACACAACTTGGTAAAATCATCAATCCTTTAGAAGATAGATTTGGCGTTATCTTAAACATTAACTATTATTCAAATGCTGAAATTGAAAAGATGGTAAGTATCTATGGAAAGCAAATGAAGTTAGAGCTAAATTCAAATGAAATTTCAGCTATCACTGAACATAGTAAACAAACACCAAGAATTGCAATTAGAATAGTTAGAAGAATATTTGAACAAAAAATTGTTAATAAAAAAATAGACCTTGAGGGTTTGTTTAAGAATTTAATGATTTATAAAAATGGTCTGCAAAGTATTGATGTCCAATATCTTGAGGTTTTAAATCGCCAAAATGAACCACAAGGAATTAAGTCAATTAGTTCCATGTTAGGTATAGACAGACACACTATAGAAAATAAAATTGAACCTTTTTTGTTGCGTGAAAATATGATTCAAAAAACCAAAAAAGGCAGGATTATTACAAATAGCGGAAGAGAATATTTAGTTAACTTTTAAGCTTATTAGCTCTTTTAATAATTTCAAAACCAAAACTTTCATTAATATCAAAAATTAATTTATCAAGTGAAGATTCTTCGCTTAATTTGCTAATTGATTTTGGTATGAATTGATAAAATAAAGATTTTTTCTGTCCTTCATCTGTATCTATCTTTTTTAAATCAAAAAAACTAATACCTATTAATCGAACGTTCTTTTCAGTATTAATTAATAGTCTGTTGAATAATCGTTTTGTTATTGACAGTAGTTTTCTATAATCGTTTGAATATTTTTTCATTTTATTTGAATGAGAATTAACAATAAAATCATTGCTTTTCAATTGCACAACTATCCCTTTACACACTTGTGAAGAGAGTTGTAATCTTATAAAGAGCTGATCAAATATTTGCGTTAACTTTTTATTTAACTGATTGTTTGAATAATTTAAATCTTCTAACGTTTCACTGACTGCAAAAGAGCGAGACTTAACCTGATTGTTATTATCTGTATATCATTTGCCTAGTGAAACTGCTTTTAAAGATTCTCAAAAATTACCAAATACTTTTTTTAAAAGCGAAGCATCTTCACAAACTGCTAGATCATTTATTTTGTAAAAATTATTTTTAAAAACTAAATCAATATGTTTTTCTCCTATCCCTGGAATTTCAGTAATTGGTAGTGGTCAAAGTTTTTTTTTAATATCCTTAACTGAACAACTTTTAATTCCAAATGGTTTTGCTTGATTTGAAAAAATCTTAGCAATTAAAAAATGATCTGAGATGCCAATTGAAATTTTGATTCTCAAGTTTTGAAAAACAAAATTTTTTATTTTTTTCGCTATTAAAAATGCCTTTTTAAATGAAATGTTTTGAAAACAAGCAACCCCTTCATCAACAGAAAGAACATCAATTTTTAATGAAAAAGTGCTTTCAATAACTGAAAAAATACGTTTAGAATGTTTTCTGTAATTACGAAAATTAGAATGTGCAAAAATAGCATTTGGACATAGTTCTAATGCTTTTAAAATAGACATTCCAGATCTAATTCCATAACTACGAGCAACATAGTTACAAGTTGAAACTACACTTCTTGAAAAGCGATTACCAACTATTAAGGGTTGATTAACTAATTCTGGATTTTCTAATTCTTCAACAGATGCAAAAAAAGCATCAAAATCAAAATAAAGAAAAATTAAATTTTTATCAATTAAATATTCTGGTTCAAAATATGTAAAGGTGTTAATCATATTTATCAAGTTATGTTATTATTTACTTGCAATAAAACCAAAGAAAATAACGAGTTTTTAACTTTAATAAAGTTATCGAGGATTTATCATCGTTTGTGCTATTGCGAATATTGTAGTTAATGGTAGATAGTAAGAAAAATAAAAAACAGCAGGTTACGGATTTTTCTAATTTACTCTCTCAAAGTAAAGGATTTGTTATTTTTGACTATTCAGGAATGTCTGCTGTTGATGCAACTTTAATGAGAAAAAAGTTGTTTAATAAGGGTAGTAAGATAAAAATTGTTAAAAACAATATCTTAAGACGTGCTTTAAAAACTAGTAATTTTGAAGGTGTTGATGAATCGGTCATCAAAGGAAAAATTGCAGTTGCTGTTGGTATTAACGAGATCTTAGAAACCTTAAAAGTTGTTGATAGTGTAGTTAAAGAAAAAGAGTTAATGAAATTTGTTTGTGGTCATTTTGATAATCGTATTTTTAATAGTGATGACTTACAAAAAATAGCAAAACTCCCTGGTAGAAATGAACTTTATGGAATGTTTCTTTCAGTTCTACAAGCACCATTACGAAAATTTCTCTATGCTCTTCAGGCAGTAAGGAATGCTAAGTAAATTAAATAAATAGAAAAATATTATGGGAAAACTAGATAAAAAACAATTAATTGAATCTCTAAAAGAGATGACTATAGTTGAAATTGATGAAATAATCAAGGCTGTTGAAGAAGCATTTGGTGTAACTGCAACTCCAATAGTAGCTGCTGGCGCAGCTGGTGCTACACAAGAAGCTGCTAGCGAAGTTAGTGTAAAGGTAACAGGATATGCTGATAATGCTAAGTTAGCTGTTTTAAAACTTTATCGTGAAATTACTGGAGTTGGTTTAATGGAAGCTAAAACTGCAGTTGAAAAATTACCTTGCGTTGTAAAACAAGATATTAAACCAGAAGAAGCAGAAGAACTTAAAAAGCGTTTTGTTGAAGTTGGTGCAACTGTTGAAGTTAAATAAAGATGGCAGTACAACAACGGCGTTCTAGTAAACACCGTCGTGATAAAAGACGTTCTCACGATGCACTTACTCTACAAACTTTAAGTGTTTGTAAGAAATGTGGTAAGAAGAAGTTATCACATCGTGTGTGCTCTTGTGGTATGTACGGTGAACTAAGAGTTAAAAAAGCTCACTAATTCACAGATAAATATTATTGTTTTTTTGCAGTTGAATTTGTATTACTTTTATGCAAAATAACAGCATTTTTTGATTTTAAACGCTTAGCTCTGTTTAAAGAAATAATCCCTTTTCTGGCTAAACGATCTGCTTGAGAATAAACAGAACTGAGATTATCTAAATTAATCTCTTTATTAAATTTTTTAACATTAGTTTTTAGTTTAGTTTTTTGTCCTTTATTATTTAAATTTCTCTTAATGTCTTGACGTAATCGTTTTTCGTTAGATTTAATATTAGCCATTTAGGTTATTAATTAGTTTTAGAGTATATTATTATCTTGATTATATTGTAACTAGAACCATATATGGATGGTGGACAACAAGGGAGTTTTTTTGGGCTTTTAGTAATTGTTATTCCAATAATTTTGTTAATTGTTTTTTTTTCCAAGAAAAAAGGGGCACAAAAAAATGATTTTAGTGGTGAAGGAGGTAATCGATCATCAAGAAAAGATGAAGTGTGAAAAACAATTAAACAGTTTTTGCAAGAAAAGAATGAACGTGGTAAAGAAATTATTAAAACTTTTGTAGCTAAAAAACCAAACCCTTTACATTCAAAAAAAGACCGCAAGCTTTTCAATCAAGAGATACAGGCATATATTACTAGTAATAACTTAGGAAAAAGCGAAGCAAAACGTTATAAAAATGAGCAAACTCGCTTAATGCAAAGAGAACTTTATTGTATTTATTTTGTTACAAAAGATGCTAAATCAACTGAAGTTGATGATGCTAGGATTATAGAAGCTGAGGTTTATCAAAAACCTACAAAAACCAAAAGTACTCCAGAGCGGCTAATTCGTATACTTGGTTTAAAAAATTTTGAAACTGAAATGCAATGAATTCAACCATTAATGGTTCGTGAAGAAAAGAGAAAAGAAAAAGAAGAACAGAAAAAACTTAAATTAGCTGCAAGAGAACTAAAAAAGAAGAAAAAGAAGAAAATAAAAAAACCAAAAGAAATCAGAAATCAGAAAAATGTTTAAAATTGTTTTCTTTGGTACTTCAACGCTTTCAAAAAAATGTTTAGAACAACTTTTTTACGATAATGATTTTGAAATTTGTGCTGTTGTAACTCAGCCAGACAAAATTAATCATCGTAACAATAAAATAGTACCTTCTGATGTTAAGTCTTTTTGTTTGGAAAAAAACATAACTTTTTTTCAACCAAAACAAAGCATAAGCATAAAAGCTGATCTAGAAAAATTAAAAGCTGATATTGGTATTTGCGTTTCATTTGGCCAGTATCTTCATCAAGATATTATTGATCTTTTTCCAAATAAAGTAATTAACTTACATCCTTCTAAGTTACCACTACTTCGTGGTGGTGCACCATTACATTGAACCATTATTAATGGTTTTAAAAAATCTGCATTGAGTGTAATTCAATTGGTTAAAAAAATGGATGCAGGTCCGATTTGAAAACAACAAGATTTTTTAGTTAATAATGACTGAAATACTGGTGATTTATCCATATATGTAGAAGAACATTCACCCTCTTTTTTAATTGAATGTACTAAAGAAATTCTCAATAAAAAAGGGAAATGATTTGAACAAATAGGTGAACCTACTTTTGGATTAAACATAAGAAAAGAACAAGAACATCTTGATCTTAATCAGATTTACAAGAGTTTTTTAAACTGAGTAAAAGGTTTAGCTCCCAAACCTGGTGGTTGGTTAAGCTTTGAAGGAAAAAACATCAAAATTTTCAAAGCTAAATATGTTAGTAAAAGTAATTACAAACATCAATTAGGAGAGATAGTTAATATATCTCGAAAAGGAATTAATATTGCTTTAAAAAGCAATGAAATTATTTCAATTGAAAAAATTCAAATACCTGGAAAAAGGGTGATAGAAGTAAGTGAAATAATAAACGGAAAACATCCTTTTGTTGTTGGTAAATGTTTCAAATAGAATGATTACAAAGAGTTTTTTCCTTAAAAATTTTGATCGCTCTAAAGAATTAATTCCTCTTTCATATAACGATGTTTTTAGCGCTGTTAATCAGTTTCTGAAAAGCTATACTGACGTTAATGATATTGATATTATTGAAGAAAATTTGATTGAAGATCCAGTTTTTGAACTTGATGTTTTAGAACTTTTAAACGAGGATCCAATGCTATTATTGGACAGTAAAAATCCCCGTGTTCAAGAAGCTAAAATTATTGCTAATAAAGCAAAAAAAAATATTAAAGATTATTTTAATCTTCCCATTTTTTTTGATACAGATAGTCTAGATAAAAATGTTTCTGTTTATCAAAAAGCTGAGCTTACTGAAAAGAAAATTCAAGAAATAATAACATCAAAAAAATCAGCTATTATTTTTAAACCTATTTTTGAAATAGAAGATTGCTTAATTCAACCAGATGCTATTATTGTTCATGAAAAAGGACTTTGTGAATTTGTTGTTATAAAAGCTACAACCAATACAAAAAGAAAATATTTTTTGGAAATAATTTATGACTTTGTGTTATTTAAAAAAATAGGTAAGTATAAACTTTTAAACTATTATTTTTGTACTGTTAAATACGAATTACAAAATAAAAATAATGTTTCTTTCTTTTTAAATACTGAAATAAAAACATCAAAAAACAGTTTTAGTTTAAGTTCAAAAGAAAAAGATTATTTTAAGAATAAACCTTTTAATCACCCTGAAAAAATTGCTTACATACACAAAAAAAAGAGCAATGGAGTTAATGGTTTTTTGATTGTAAAACTCATTGATAACCTTATTAAAAACAATATAGTTGATTTAAACAAAATAAGTGATTTTGTTACTAAAGAAATTGACAGTAAAAGTGTTCGTAATATTCAACCACTTATTAAAAATGCGGCTAAAATACAAATTAATTTTTGAGATCAAATACAAGATATAAAAAAATATCAAGAACTCAAAATAAATCAAATTGTTTTTAATTACAGTGAAAATTTTGATTCTTTTTGGAGTAACTATTTATTAAGAAATTTAATTAAATTAGTTTTTGCTCATAAATACAATGAAATTTTTAAATTATCTGGTAAATTAGCTAATTGAAGTCAATTAACATATGCATATAAAGAAAATAAATCAATAACTATAAATCAACTGCTTCACGAATTAAATCAGAAAAAAAGCAAGGCTAATTTTAATAACTCAACTAATAAAATAAGTTTTTTTCTTGAAGCGTGAAATAGTGAAAAAGGTTTTGCAATTGGCAATAAATTTAAAAATACTTGAAATAAACTTAAAAAAAGAAAGTTTATTTTGATTTTGAAACAATTAGTTCATCAATCAGAATCATAAATAATTCATTACCATTTAGTCAAATTGTTACCCAATGCTCATTAATAGTTGATAAAAATGAAATAGATGATAAAAGAAAACTTAATTGTGAAAATCTAATTTTTGATCCCTTATTTATTAGCGTTAATGACTTTAAAAAAGTAATTGATTCACTCTATCAAAACAATTGCAGTGATTATAGTTTTGTTGTTTTTAATAAATCTTTTGAAAAAAACAGATTATTGGAAATGGCTACATTGATTAATGAACAAATATATAAAGAAAAAGTAAAAGCTATTGTTGACAATCTTTTTGACTTAGCTGATATTTTTACTATTGAAAATAACTGTTTGGCTTTCAAACAGCTAAACGGTTTTTCCTCTATCAAAAAGGTTTTAACTATTATAGATGAAAGCTTTTTAAAGGCAAGTAAATCGATTGGTTATCAAAATTTAAAAATCCAAAAAGGTGATGTAGCACAAGAAGTTGCTTTATCGCGTTTTCTAAATTGCTTAAATAAAAATGAATGAAATCAAGTAGCTTTTGAATTAAAAAAATATTGTGAAAATGATGTCAGAGCGATGATTTCAATTGTTTTATTTATCCAAGATTTAATCAAAAAAAATGATCTATTTACTTTTTATTCAGAAAATTAGAACCTGCTTTTCTACTATTTCACCTAATTTTTTTTAGGTTTAATAGCTAGTTTCTTACACATCTTTTGATTTTCTATCTTCTCTTTTAAATCACTAAAAAAATGACTTCCTTTACTACTTTTTAAAAAACTTGTAAAAGAAGCCATTGCGTTGTGCTCTAAAACACTTTTATCATCGTAATTTTTGTTATTTGGGATAGCTTGTGATCAAATAATATCACCATCAAGTTCAATTATACTAAGTACCAAATTATTTGAAGTTAAGCTTACCCTAACTCTTGGTTCAGGTAATTTTTGCTCTTGAAAAAGTGAAAAGAAATCGTATTTTAAAATCTCAGAAAAAGTTCTTTCAAAGATATATTTTTCAACAAATTCAGTTGCTTTTTTCATCCCCATATCTTCATAAATGGCGCCAACCAAAGCTTCAAGTACATCACCAACAGTGTTTTCAGTTAGTTCAGCACCATTGCTTAACTTAACAAAATCACCTAATTTTAGCTCCATACCAATACGATTAAGATTTTCACCCTTAACAATTTCAATCTTAGTTCTTGTTAAAAGACCTTCGTTATATTTAGGATAAAGTTCAAATAGTTTTTTAGCAACAACAAAGTCAATTAAAGCATCACCTAAAAACTCTAAGCGATCGTAACTTTCACTAACATCTTCATGTTCATTGATGTAAGAAGCGTGAATAAAAGCTTTTTCAAAAAATTCTCAATTATTAGGAAAAATATCTAAATTCTTTAAAAAAGTTGCTAGTTTTTTATCAAAAATCTTATTATTTTTTAGTTTCAAAACTTTATTCTTCATAGTTATTCAAAGAAGTTTTAATTGCATTAATAAGATCAGATTTTAAGCTTAGATGTGCTAATCTAATCGTGCTTAAAAATTGTTGTTTATCAGCAGAACCGTGAGTTTTTAAAGCTAATTTATTTAAACCCATTACAACAGCTCCAGCGTTATTTTTGTAATCAAATTTTTTGGCTACACTTTTGATAATACCTAAGCTAAATAAGCCAGCAAGAGGATTTCTTTTATAACCCCTCTTAAGAATCCGTGCTATCGTTTTAAAAGTACCTTCCATTGCTTTTAAAACTAAATTGCCGCTATATCCATCAGCTATCAAAATATCACATATTCCATCCAGTAAAAACCTTGATTCTACAAATCCTAAAAAGTTAAGATTTTTATCAGCTTTTAAAAGCGTAAATGCTTCTTGATGATAATCAAAACCTTTATTTTCTTCTGTTCCTATATTCAATAGTCCAATTTTAGGAGTTTTTTTTGTAGTTGTTTTTTTAACAAAAATATCAGCCATTAAACCTAAAAAATAAAGTTCTTTTCCTGTGAAATATTTATTAGCTCCAACATCCAAAAAATAAAACCAATTATTGTTATCTGTCGGTACATAAGACATAAAAGCACTCTTAGTATTTTTATTAATTTTGCCAAAAGCATCATTTGTTAAAGAAGCATAAACTGCTGAAGAGCCTGCTGAAATTACAACATCAGCATTACCTTCACGAACTAAGTTTATGGCTATTTGCATTGAACTGTTAACTTTTCTTCTTGCACTTAGTGGAGTGTCGGTCATTTCAATGAAAGAATTAGCAAGTTTTTTTGTAATATTTTTTGGAAGTATATCAAGACCATCAAAAGCCTTTTCATCACCGATCAGAATGAAATTTAAGTCCTGATGAAAACTCCAATATTTCAATACTGCTTCAATTGCTTCACTAGGTTTGTTTTCAAAACCTAAACAATCAACTGCAAATCTAAAAGCCATTACTGTATGCCTATTATGTAAGAGAACACTTTTTGTTCTCCAGGTGAAAATTCACAAAACAATTTATATTTTTTTTCAACTAAAGCTTGCATTTTTTTCGCATCTTGTTCAGTGATGTCTTTGCCATAATAAGCCAAAAGGAACTCAGGTTTTTTCACTTTTTTTGAAAGGATATCAATTGTTTTAAAAAAACAGTCTGTAAGTTGAGATTCACTAGCAATAATACTTTTATTTGTAACAGCAATAAAATCATTTTTATTCACCATTACTTTGTTTTCTTTATATGACTTTGAAGCTTGTGTTATCGTAGCGGAAGCAAACTCTTTAATAAAGCGCTTCATTGCTTTCACATTAGTGTGAATAGAAAGATCACTGTTAAATACAGTTAAAGCAGCAATTGACTCCACAGGATTTGCTGTAATAACATAATCTGCATTAATCTTTTGGTGTTTTAACTGTTTAATTGTTTCATTGGCAGATAGAAAATAGTTTTTATCATGTAACAAAAAGATTACATTACTAGAATTGGTTAACTTAACAGCCTCAAGTAAAGAAAAAACAGAAGGAGCCCCAGTATCATCAGTACATAATATGGCGTTAATATCATGATCTTCACGAATTCTTTCAGCAAATGCTTCTGTAGGTACAGTAGCTACTATAGCAGGTTTTAAAACTCTTTTTGTTGGTGAGTTATTCTTGTTATTAACCTGTAAATTCATGTTATCAATTTTGACAAATTCAAATTCACCATAATTAAGACCCATTTCTAATAAAAGATGTGGTTTTAAAGTATGAGCATGAACTTTAACAAAACCATTGTCTTTATCACTAGCAATTACTACAGAATTGGCAATTCTATTTACTTTACTTTCAAACTTTTTTTGATGAAATTTTTGCTTTTCAACTGTCTGATTTATTTTTAAACCTAGTCTTAAAACATATTCTGTACAATAACCAAATTCATCTTCATTAGCATGTTGTTCTTTAAACTTGTTAAATTTAATTTCTATAGTTGATAAAGTATTGTCATCTAAATTAGATTCGCCCCCATAATATGACAACATTCCTTCAAGAAAACAAGCAAAACCATAAGCACCTGAATCAACCACACCTGATGCTTTTAATACAGGTAACATTTGGGGAGTTTTTTTCACTGCTTGTCAAGCTACTTTAACAGCTTGTTCAAAAAGTTTTTCAAGTGTTTTAGGACGATTTTTTTGACTCTTAAACTCATTGCTTATTAAACGAGCAACTGTTAGCATAGTTCCTTCTACTGGTTTTGATACGTTTTTATAAGCCACTTCTTGAGCAACAATGAATGCATTTGCAACATCTTCTATACTCAACTCTGAATTATTGCTTGTTTTATTTACGATTATGTTACTGAAAAATCCTTTCATTATTTGTGAAAAGATAACCCCTGAATTGCCACGTGAAAACAAAAGTAAATCTCTAGTAAAGTTTTTACCAAGTTCACTAAAGCTTTTTATATGACTTTTTTCATTTTCTAGTTTCTTGATGGCTTCTGTTATTGTTACTTTCATGTTTGTTCCAGTGTCACCATCAGGAACAGGAAAAACATTCAACTGGTTTATGTATTCATAATTTTTAGCGATGTTATTACAGCCAAGGCGTAACATATCGATAAAAGAAGATAAATTTACTGATGACATCTAAAGTAAAACCCTAGCTTATAAGGGATAAAAAAGAAGTTTATAACCACAATAATTTTAAATTTTAACTATTGAATTAAATATTCAAACCAAGGTACTATTTTACTCTTGAATTGCATTAATGAATAATTTGTTAAAATGGGAAACTTGCTCGTTTCTAGTTTAGGAAAAACTAATTCATAAGCGTGAAGTGCTTGATGCTTATATTTATTATTACGATTTTTAAATTGAATCAAGCTGTATTTTTTATCACCAACTAGTTGATTTGAAAATAAATTTAAACACGCACGAATTTGGTGAGTCCTACCACTAATTAATCTAATTGTTATTAAAGACATATCTTTTTGAGCTATGTATGAAGAATTTTCAAAAAAAGTGCTTATCTTTTTTGCTTCTGGAAAAGGTTTTCATTTTACTGTTACAATGCCATTATTGTTATCTTTTTTTCAATAAGCAGTTTGTAAGCCAAGATGGTTAAATTGACCAAAAACTAAGCCTTTATATCTTTTGGTTAAATGGTTATTTTTAAAAACTTTGTTCAATTCTTTTAAAGCTTTGTTTGTTTTAGCACCAATAACAATTCCAGAAGTATTACGATCAATACGATGTGCAAACTGGGGATAAAAGTTAAGTTTATTACTGTCAAATTGTCTATATCCACAGTGTTTTAAAAGCATATTTGATAAATTAATAATGCTATGTTTTTTATCAGGTTGGCAAACTATTCCAGTTGGTTTGTCAATTACAATAATATTTTCATCCTCAAATATAATTTTTAGCTTATCTTTTACCAAATCTAGAGATAAATAGTCATTATTAGTTTGTAAATAGGGATTTATATCAAAAAGAAAAGCTATCTCATCTTTAGTTTTTAATCTTGTGTTGACCTGTGGTTTTGTTTTATTTAACAAAACCTTTCCTAAGCGTAAATACTTAAAAAAGTCACTTCTTTTAATTAAGGGAAGGATTTTTAAACAAAATTGGTCAATTCGTTGGTTTTCACTTTCTTTAGGAACTAAAAATTTATTAGCTATTCTCATTTTGTTTGGCAAGAAAATCATCCATTATCTGTACTAATTTCATAAATTGATTTTTTTCATCCAAAACAAAAGCAGCTGCATATTTATGTCCACCACCATTGAACATTTGTGCAAAGTTATTAATTGGAATATTACGTGAGCGAATGGAACCAATTCATTTTTTAATGGACTCGTTAAAGTAAACAGTAGTCCATATTTTTACTCCTTTTATGTTATTCAAAGCATGAACCATTGGTAAAGGAGAGACAACACCAAAATGTTTATATGCTCCTTTTTTAATCAATGCATAAGCTAACCCATTTTTAGTTATTTTGGCTTTGCTAAGTACATAGCTAAAGTACTTATGTTCTAGTAATGGCTTTAAATAAACTGCATCATGAACCTTATTGCGGTTAAAACCAGTTTCCATCAATTTTGCTGTTAGGGCAAATGTTTGGGGTGTAGTAGTTGGACCTCAGAATCGCTGTGTATCAGTAATTATTCCTGCATAAAGATATGATGCAATCTCATCATTTAATTTGTAACCCATCTGCAAAATTAAATAACCAATCATCTCAGCAGTTGCTGAAAAAGAAGAATCAATTCATTCCATATCAGCAAATTTTTCAGTTCTAGGATGGTGATCTATCCTAACTGTCTCTTTTGCCAACTTATGTTTTTGAGTTAAAACTCTTTCTTGGTTGGAAGTATCAAAAATAATAGCTAAAGATTCTTTTACAAAATCATCATTTATGTCAGTTTGCTCAAAAGGAAAAAGTTCTCTACCGTCTGCATTAATATTGTATGAACCCATAACATAAGCTTTTTTTTCACTGAAAAAAGTATTTAGGAAAGTTTTGAAAGCAAAAGCAGATCCAAATGCATCAAAGTCTGGGTTTACATGTACAAATAAACTGAACTTATCAAACTGTTTAACCTTTTTTGAAAAATTCTTAATGAATTGTGGATCGATACTAATCATTGCTTAATTCACTTACTCCCTTTTCAATTAAATCATTTAAAAAAATAAGCTCACTTTCAAGCTTTTCAACATTTATCAATTTAGCTTTCGTTGTAGGATTTTTGGGAGAAAACTCACTACAAGTATCTAAATGTTGTTCAATAGAAATATCAAAAGTATTAAAAAATTTAGCTAATTCAATAATTTTATCTTTACTAAAGCCAATTAAGGGGCGAACTATAAATGTATCTGGTGTTGCTGATTGAATAACTTTTAAATTTTCAATGGTTTGGGATGCAACTTGTCCCAAAACCTCACCTGTAACTAGACAATCATACTTAAACATACTAGCAGCTTTATAAAAAACACGACGCATTAAAACGATACGATAAGATTCATTACTAATATGAATTAACTCTTTTTGAATTGCAGTGAAATCAAAAACTAATAATTTACCACTACAAATCGTTTTATTAAAAGAAATTAAATTTGCTAACCTTGTGATTTTTTCAATTGTTTTTTGATTCTTATTTGGTTCATTAATAAAGGTGATAAAATCAATGTTAAAACCACGTTGCATTACAAGTGAAGCTGCAACTGGGCTATCAATTCCTCCTGAAAGTAAGACTAAAGCTTTACCACTACTATAAACAGGTAATCCACCTTTACCTTTAAAACGTTCTGTAAAAACAAGAAAATGCTCCTTCAAAATCTCTATATTAGCTATTATTTCAGGGTTATTAATTACTCCTTTTAATTGGTATTTTTCAAATAGTTTAACTGCTAAATACTTCTTAAAATTACTTGAATTTTCAGCAAAATTTTTATCACGTCTCTTAACTTCTAATTTAAAGGAATTAAAATCTTTAAATAAATTAAAAAGAAGATCTAATAGTTTGTTTTCTTCCCTTACTATCTGTGATGCAAAAAAGAACAAACTAATTCCAGGCAAAAAACTGAATAATTCTTGGAGAATCGCTCTTTGTTCTTTTTTAATATCAAAGACAACAATTCTATCAAATTCATAAACAATACTATTGTTAATTTCCAGTTTTTTAAAAGCTTTTTTGATGTTAATTTTTAATTGTTTTGTGAAATAAGAACGGTTTTTCCTTTTAAAACCAATTCACCATAACGCGCAACTAATACATCTTCACTATTCAATTCCATTTTCCTTTAATCATTGAATATCACTTTCTGTTAGTTTAGGACTTTTACCAGTTAATATGTAATTTTCATATCAAATTCTCGCCTTATCAACTAAAACACTAAATCCCTTATGATCTATCTTGATAGGAACTAACCTAATTTCTCTATTGCTAGCATTAAATTGTTCTGGTAAGACATAATCTTCTGGTTTCAAAAAGGCAATTGCAAAAAGTCCTTTATTTACATTGCGCAAATATAGATAAAGACCAATTTGACAATAATATAAAGTACTGATAATAATTTTTCCATTACTGTCAAATCAACTATCTTTTTTGCCATCTTTCTTTTTAACAATAGGCATACCATTTTCATCAAGAATCATCTTGAGATTTCCATTGATTTTTTTATAAATAAGACTATCGCAAGAAGTTGTTTTAATCTCTAACATAGGAAGATCATTTTGATAAGCTAATTCACCATTTTCATCAAGAGGTTCACCATCAGGTATCCCACCAAAAACACTATCATCCTTAAATAAATCAAATTGAACTTTTTTAGGATCATAAGAATGAAAATTAAAACCAGTTTTTAAATTGACATAATCACGTATTTTAGGTTCTATTATGTTTCCAGCTTTTGCCAGTGTTTCATCAAATTCATCTTCATAAATTTTGACCATATTAGCTCAAGTTTTAAATGATGACTCATATTCAGATAAACCTAAAACCTTGCCAAAGCGAGTACCGGTTATTTTTTTAAATAGAGCTTTATTTCTTAAAAAATACTGTTCTGATAGAACAATTTGATTATCAACTATATCAAAATCAGTTTTATATTGCTTTATAAATCCCATTTATTTCATCAACTGTTCACAAATTCCTTTTAAAACAATTGATATTTCAGGATTATTAAATTCCTCATCATTATCTGGTTGTTTTTTGCTTAAAAATACAACACTTGCTAATTGATTAATGTTATTAATTGGAACTTCTGTCACATTTAAATAATGAACATCTTTTAAAGAGCAATTCTTGCTTTTAACATATGTTTCTAAAGCACTAAACTTGGAATTTTTTGTGCTAGTAAATAAAAAATTTTCGTTATAAAAATTGCTGTAATGAAACTTAGGCTGACCACTAGAAATATGTTCCATAAGTTGAAAGTCTAAATGATACTGAGGACTAAATACATAAATAGAACTAACATCCATTTCACTGAGATTTTTTAAGAAATTTTGGTACTTTAATGTTTGGTCAAATTCCGTTAATCAAATTAATTTGAATTGCTTATATTTTTCTAATGAAATAAAGTAATTAATTGTTGTATTAGGTTCCTCACTAATCTTGCTTTTTCCAATTGCAAAAACACCAGAATTAGTAATAATAGCATGGTGTACAATAGTGTCTATACTAACAGAATCTAAAAAAGATTGATAGATAAATTCATTGGTTTTGCTAATTCATATAATCGCGCCTGATTCTGCAATTATATCAACATCATCTAACGAGAGTAAATCAAGTAAATAAAGCACTGCTTGTTGTCAACAACTTGACATAAAAACAAGTTGATGGTTATTCGCTTGAATGAAATTAAAAAAGTTTTTTCATACTTCTAAATCCTTTGTAGTGTTAAAAAAATTTGCTGTATTAATATCTAAAACAACTATTTTTCTATCCATCTCTTCTAACTCTTTTAACAAAATTATTAAAGCTAATTCTGGTGATTTTTTCACCTTTAAAACCACGGCATGTCAACTTCTCTAAATTCTCTATTTCTTTATCTCCAACAATAAGTTGTAAAGGAATTTTTTCAATGATTGCTTGTCTAACTTTTTTAGCTAAGCGATCTTGATTATCATCTAAATTTACACGGATGTTTTCTTTTAGCAATTTGTTATAAAGTTTTTTTGCTGCCTTTAAATGCTTTTGGATATTAACAGGAATAATTACGGCTTGAACAGGTGCTAACCATAAAGGAAAATTACCACTTGTTTTTTCAAGTAAAGCAGCAATAAACCTTTCATAAGTTCCAATAATTCCAACATGGATAATAACTGGTTTTTTTAGTGTATTTTTTTTATCTATATAAGTTAGATCAAATTTTTCTGGTAGTAAAAAATCTAGTTGAATGGTGGCAATAGTAATCATTTTTTTAAAGATTGTTTTGAACTGAAAATCAATTTTTGGTCCATAAAAAGCAGCAGCTCCTATCTCTTTTTGATATTGGATATTTAAATCTTTTAAAACATTCTCCATTTGGCTTTCAGATTCTCTTCATAAACCAGGATTATCAATAAATTTTGATTGATTTTTAGGATCATGTAGAGAAAGATCTATCCTATCAAATATAAATCCAAATTTTTTATTAACTTTTTGAATTAAATTAAATGCGTTTTTAATCTCACTTTTAATTTGATCTGCACGACAAAAAATGTGATTATCAAGTAAAGTCATGCACCTCACTCTTTCTAATCCTATTAATCCTCCAGAGGCTTCAAAACGATGCAAAATAGAATCTTCTGAAAAGCGCTGTGGCATTTTTTTATATGAATATCGTTTTTGTTTGAAAATCAGACAGTGATGAGGACATGTCATAGGACGAAGCATCATTGCTTGACTATCAAGTTTAATAGCAGGAAACATATCTTCCTTATAGTGCTGATAGTGGCCGCTAATTTTAAAAAGCTCTATGTTAGCTAATACAGGAGAACAAACAGTATTAAAACCAAACAATAGTTGCTGGTGATGCACAAAATTACCGATTATATTTCTTAGTGTTGTACCCTTTGCTAATCAAATAGGAAGACCTGCACCGATCAGTGGGTCAAAGCTAAATAACTCTAATTGTTTACCTAAAGATCTGTGATCCTTCTTCAAGCGTTCTTCATTTTCTTTTATTAATTGTTCTAATTCTTTTTTTGATTGAGCAAAAATGCCATTAATCCTCTGAAGTTGTAATTGTGAAGGATCTCCCAAAAAATAATTTACGCTTACATTTAATAGCTTAATAAAACTTTTTTTAATAAAAGTTAAAGTTAAATCTTCTATTCAAAAATGTTTATTTTCAAAAAAGGTGATTTTAAATTTGTTTAAGTTGCTTTTTTTTAATAAGTTTTTTGTAAACTGATCATTTTCAAAAAAACTTAATGCTTCATCTAAAGAAACAAATTTTTGAGAAATCCCTTCTAGTTTACTAGAAAGAGAGTTTAAATCAGATTCTATTTTTGCAAACTGTTTTGTAGAAAAATTCTCATTTATATAAAAATCTAAATAAAATTCATCTCCATTAAAACCATGTTCACCAAACTGAACATTAGCATATTTGCATTTTAACCATATTTGTAATAAAAGAACTCCTGCAAGCATTAAGATTCTTGATAATCAATCATTACTTTTGAATTTGGTTTCATAATTTTGACAACTTCGATTGTTGTTTTTCAAATTCCACTGCGTTTTTCTAGGTAAGTGCGCAGAATACCTTCGATGCTAATTGTTTTAAATTTTTGAGTATATGCTTCAAGTTCAAATGCTAATTGACCATTTGCATAAAAAACAAAAAAATCAGTAAAACTTCGTTCACCAAACAAACGTTTCTGCTTAATAGTTACTAAAAATCCTGTCTTTTTGTGGCTTCACTTTGTGCTTTCTATTTCACCTTCCAAAAAAACGCAATTTAACATGCTTTTTAGCTAGGTAATTGACCTGTTAAGTTGAAATGAAATTTAAAGTGGTTAATTGTTTCTAAAACCATTCTTTCTTCTATTAATGCAGTTCTAATACCTTCAAACTTTTGTTTATCATCAAGATATTCTCTAACATTCTGATTAGTTTTTTCATAATACAAGCTAATAACTCTTTTAACAATATCATCACTAACTTCAACCTTTCATTCCTTTTGTAAGAAATTAAATACTAAAGCTTTTTTAATGATTTTTTCAGCTATTGATTGAATTGTTTTTTCATCAGCATCTTTAACAACATCTCTTTTCAAGCCTTCAACGACATTTTTTAACTCTTCAGGGTTAATTTGAAATTCAAAATAACTAACAATTTCATTCATTGCTGCTGAAAACAAATTATCTTTAATAACAATATGAGTTAGTTCGTGGTAAAGAGCACTTTCCTTAGCATCTTTAAAAGAAGCTAAAATTCTTTGCCGATGTTGCTCAATAAAAGCAGGATCTGCAAAAATACGATCAACTTCAATTACCTTATCATACTGAATAGGTTTTTGCAACTTTGCAATAGATTTAAGATTAGTAGCCATATTTTTAGAAACTTATTAAATTTTAATTCCTATATGCGTGAAGAATTTACATTTGTTGTGGTGTTGAAATCTTCAAAACATCAAGGATTTGTTTAACTATAAATTGTAATGATCTCAATAAAGCAAGTCTTTGTGCTGATAAAAGTGAATTTTTATCATCATTAATTTTGCAAACTTTATACCAGGAATGAAATAAACTAGCAGTTTCATATAAAAAGTTAGTTAATAAGTGCAATTCGCCTGTTTCCATAGCTTTTTGCAACATAAAAGGATAATAGACAAGTTGATTTAAAAGTGATATCTCTTTTTCATTAATCAAAAGACTGCAATCAGTAATTTCTTTTAATTGATCAGAATTTGCAATTCTAAGAATACTATTCATTCTTGCAAACGCATATTGAACATAATAAACTGGATTAGCAGAGTTTTGCAAATTAGCTATATCTAAATCAATTTTGATAATTGTGCCATTATTTTGAGATAACATAAATCATCTAGCAGCATCTTCACTAATCATTGAAAGCATTGTTTCAATTGTGATTACATTTCCAGCACGTTTTGACAAACGTACTAGCTCTTTATTTTTATAGAGAGTAACCAGTTGTAATGCAAAAATTTTTAAAGAAGTTTTAGTGGTATTTTTTAAAGCATCAAATGCACAATACATCCTTTTAATATGTCCTTCATGATCAGTGCCTCAAACATTAAATAGAATGTCAAAACCACGTTGTAATTTTTCTAAGTGATAAGCAACATCAGTTCCAAAATAAGAAGCTCTTTTATCACTTCTAATTAAAACTCTATCCTTATCATCTCCATAAAGCGTAGTTTTTAGTCAGAGTGCATTATCTTGATAAAAATAACTATTTGGTTTAAGTTGTTCTAAAAGATCATTAAAATTTGTTTTTTTAACAATTTCGCTTTCAAATTTTCAACTATCAATAAAAACATGGATTTTATTCAAATCACTTTTAATTAAAGACAAAACTTTTTGGGTGCAATTAACTAAAAAATGATCTAAGAATGATGTTTTACAAAACTCTTCAAAATTTAAATTATCAACTGGAAAATTTTCAATTTCACTAGCAATTTTATCTATTACTATTCCACTATATCCATCAGGATGTTGCTGGATAGCAATATTTTTTTTTAACTGCAAACTTTGATAAACACTAAAGCTAAAAACTCGTGCTTGTTGTCCATAATCATTTACCCAATATTCACAGACTGTTGTATAACCCAACAGCTTGGCTAAATTGTTTAAAACATCACCAAAAAAAGCTATTCTCACATGACCTAAATGGATCCTTCCGGTAGGATTTGCTGATACAGATTCAATTAAAATCTTTATATTTTTCTTAGTAAAGCAAGGTGTTTTTATCAAGTATTCTAAGTACTTTTGATAGCTAATTTGAAAGTTTATAAAATTATTGTTAGCAATAAAAACATTTTGGTAGTTATCCTTTTTATTTTTTAAAAATCACTCAACAATACTATCTGCAATTTTTTTATGATTTTCACTTTTTAGTTGGAAAATAATAGTTGAAGAAAAACCATTAAAACTATTATTTTTAACTAGTTTAACAAGTTCCTTTTGATCATCAAATTTAAGCTTTAAAGCGCTAATGCATTCTTTTAAATCATTGATGATAAAAAACATTACTTTAAATTATATTTAATGATGCAAAATGTCTTTTATCATAACAGTAATAGGTGCTGGGCATGCTGGATTGGAAGCCGCTTTCATTGTAAGCAAATTCAACATCAAAGTAAACCTTTTAGTTCTTGATATAAATCATTTAGGTTCTTGTCCATGTAATCCTTCAATTGGTGGACCTGCTAAGGGAATTGTTACTAGGGAAATTGATGTTTTAGGAGGTATGCAAGCAATTGCTGCTGATAACAATGCCTTACAATATAAATTACTAAATAGTTCAAAAGGACCTGCTGTGCAAGCTATCAGAGCACAAATTGACAAAATAGGTTATAAAAACTGGTTTCAAAGTCAAGTTAAATTAAATAAAAACATTAATCTAATTCAATCTGAAGCAATCAATTTAATTGTTAGAAATGAAAAAATAAAAGGCGTTATTTTAAAAGACGGAAGTGAACTTTTAAGTGATGCGGTTATTATCACTACCGGAACGTACCTAAGATCAAAAACATACTGTGGTAATACAGTTAAAAATCAAGGACCTGATCAATCTAAAAATAGTGAAAAATTAAGCACAAACTTAATTAACAGAGGTTTTAAAACAATTCGTTTAAAAACAGGAACTCCGCCAAGAATTTTAAAAACTTCACTTGACTATAATCAAATGGAATTAGAAATTAATAATAATCAAAACCTTGCTTTTAGTACTACAAATAAAAATTTCTTACCACTTGAAAAACAAATACCTTGTTACTTAGTTCATACCAATCAAAAAATTCACGATCTAATCCTTAAAAACTTAAAAAAATCTGCAATGTTTAATGGTAGTATTTCAGCACAAGGACCACTTTATTGTCCAAGCATTGAAGACAAAGTTTTTAAGTTCTCTCAAAAACCTCGTCACCAAATTTTTGTAGAACCTGAATCATTGAGTCTAGATACTATTTATTTAGCAGGATTATCAACTTCTTTTACACCAGAAATTCAAAAAGAAATCATCCAGCTTTTACCTGGTTTTCAAAATGCAAAAATTAAAAAGTTTGGTTACGCTATTGAATATGATGCTTTTCTATCTAATCAACTAAAACCAACACTTGAAACGAAGTTAATAGAAAACTTGTATTTTGCTGGACAAATTAATGGCACTAGCGGTTATGAAGAAGCTGCTGGTCAAGGTTTGATGGCAGGAATTAATGCTGCTTTAAAATTATTAAAAAAACCACCATTTATTTTGCAACGTAATGAGGCTTATATTGGGGTTATGATTAATGATTTAGTTACTAAAACAATCAGTGATCCATACCGTTTGTTAACATCCAGAGCAGAATATAGACTATGATTGAGAAATGACAATGTTCAAGAACGGCTCATTAAAAAAAGCTTTGAACTTGGTTTAACAGATAAAAAAACATATGAATTGTTCCTTAAAAAGGAAAAGAAAAACAGGAATTAATTTCATTTTTAAAAAACACTCAAGTAGGCAAGGTTAAAGCATTGAAATTCACTAATAAAAATACCGCTCAATCACTTTATGACTTCAACAAACGAAGTGAAATAAATTTAGATAAATTGATCAAAGATCTTCCTGAAAAATACCAATTAGATTCAGAAACACTTAAACAAATTGAAATTGAAATTAAATATGAGGGTTACATAAAGAAAAATGAAAAGTATTTTAAGGGTTTAGATAAATTAAGCAAAATTAAAATTCCTCATACTTTTGATTACCATAAGGTTAAGAATTTAGCTAGTGAAGCTATTTTTAAACTATCTAACTTTAAGCCTAGTAATTTAGCAATTGCAAGTCAAATAGCTGGAGTGAACTTTAATGACATTATAGCCATAAAACATTTTTTAAAAACTTATGAATAATGCTAATTTTGAAAAATATGTTGATTTAGTTTTTGAAGCAAACAAAAATTTCAACTTAACAGGATTTAAAACAAAAGAAGCTATTTATCAGAATTTAGTTATAGAAATATTGACATTATTTAAAGGATATGAAAAATTTTTTATTGACAAAACTGTAGCAGACTTGGGAAGTGGAAATGGTTCGCCTGGGATAATATTAAAACTGTTATTTCAAAAAATAAAAAAGTTAGTTTTAATTGATAGTAAACACAAAAAAATTAGCTTTTTAAATAAATTAACTAAGCAACTAAATCTGGAGAAAACTGTTGCAATTTGTGAACGAATTGAAGTACATAAAAATCACTATGATGTTATCTGTTCTCGTGGTCTAAGTACGATTATTAAAGTTAATGATTTAGCATTTTCCTTGCTTAACTCAAAAGGTATTATTTTTCATATAAAACAAAGCTTAGACCAATACATTGAATTTGAAAAATCAAATCAGAAGAATCAATTTAACTTGTTATTTATAAAGCACTTTACTAGTCAGAATAAAAAACTAATTTTGATAGCTTTACAAAAAAATGATTAAGTTAGGTTGAGCGTCTTTTAGTAATACAGAATTTGCTCAAGATATCTTTATAAAGTTTGCCAATCAATTCTATAAACAAGATGATGCTGGAACAATTTTATTTGAACTAAAAAAAACATTAGGAGTTAATCAACTAGATGAAATTGAAAAAAATAAAAAAGTTATTATTGTTAATCAGTTTCAAAATAGCTTAGGCAAATTTTTACTTTTTAATAAAGAAAATACTAAAAGAATAAACAGTTTAGCTAATGAACATATAAGTTCTTTTTTAAAAAAAATTTTCAGATTATCTGGATTTAAAGATATGTTGATTGATGTTCAACATCATAAAAAGTTACAAAAATGTTTATTAAGAGAAATTCATCTTTTAGTTTGTTTAATTAATAGCAATCAATTTAGTGATGAATTAATGCAAATTATTGAGTGGTATCAGTATTTAAAAAAACACTCTAGTAAACTTTTTGTTATTACTGCAAGTAGTGATAAAAAACCAGTAATAGAGCCAACTATAAATGAATATAAAGCTATTTTTGGAGAGTATTTATCTTCATTTCACCTAGATCTAAAAAATAATCAAAGTAATGATTTATTCCAGAAATTACTTGATCAGATCAAAATAAAAGCTACCTCAAAAACTAGTTTGAGATAGCTAAAAATATAAATGGCGCATCGGGAGGGATTTGAACCCACGACAACACGCTTAGAAGGCGTGTACTCTATCCACTGAGCTACCGACGCATTTCTTTACTAGAAATTATCATATGAATCTTCAATAAGACTCTAATAAAAGTTTAGATGATTTTTAACTTTTTTATTGTATTCATGCAATAAAAATTAATTTAAATAAAAGAAATTTATTAAAAAATCCTTAAAGCACAAATATCAATAATTTCCTTTTCTATTGATTCTTAACTATTTTTAAAAAAATAGTCAATTTATTTAGTCACAAAAACCTTAGTTAAAAATAAATTTAAGTTGCAATTGGATTGAACATATTATTTAAACTTTCTTTTAAATTAAGTGTTTTAAAAGTTGATTTTTAATAAATTAATTAACAAAATAAGATAAATAGTTAAAAACTCAATAATAAACGCTTTAAAATATTTCACTTTGATGGATGAAAAAGGGATTTTAGTTGCAATTAGTGGTGGTAGTTGCTCAGGAAAAACTACTGTTGCTGAAATGATTTATCAACTTTTAAGTAAAAATTAAAAGTTGCGATCATCTGTCAAGATAACTATTACAAGTCCTATAAAAATAAGCCATTATTAAAAAGAAAAACAATAAACTTTGATCATCCTGATGCTTTTGATTGAAAACTTTTAAGATCACACATTGAAGATCTTCTAAACGGTAGTATAGTTAATGTTCCTTTATATGACTACATTAACTATACCAGAGCTAAAAAAACAGCAAAAATTGGTCCAATTGATGTTGTTATTCTAGAGGGTTTAATGCCATGATTTGATGAAAAATTATCAAGACTTTCTAAGCTAAAAATATTTATAGAAACAAATGGGGAAGAACGTTTAATTAGAAGAATAGAAAGAGACTGACAAAGGGGAAGAAATATTGATTCTATTATTAAACAGTGACGCGAAATAGTAGCACCAATGTATGAAATATTTGTAGAAAAAATGAAGCGAAATGCTGATTTAATTCTGCCTTGAAGTCAACGCAGAGAAGTAAGTACAAGTGTATTGGATGTCGCAATTGAACACTTATTTCACAAAACTGTTGAAAAAAATAATTAGTTCTGGAAATTATAGAGATGTTTAAATTTCAAAGGTGAATTATGTTTATGCTCTGCTTTTGCTTTCAAATCTAAAACAATTTTTTGCTTTTCTGAACTAAGTATTAAATCACCTTTTAAATATTGATCAAGTTCCTTATAAGTAATGCCCATCTCTGTCTCATCAGTTTGCCCTTCAAAAAGACTAGCAGTTGGCGCTCTTGTGATTACAATTTCAGGAATATTAAAATGCTTAGCTAATTTGTAAACGTCCTCTTTTAAAAGCCATGCTAAAGGAGCAATATCACAAGCTCCATCTCCTCATTTTGTGAAATAACCAAGTGTATACTCTACAAAATTACCAGTACCTAAAACTAAGAAGTTGTGTTTTTGAGCATAAGCATATAAAGTTATCATCCGTAAACGTGCTTTAATGTTACCAGCTGTTAAAAAATCTTTTTTTGGATCTATTCCAAGGGTTTTTACTAACAGATTGAAACTCTCTTCCAGTTCAATGTTAATACTATTAAATTGCATTTTTTTAACAAGTTCACTAGTTGCTTGAAAATCAAGTTTTGAATTATTAATATGCATTATTAAAGCTAAATGGTTTTCAAAACCAAAAGTTTCTTTAGCAATAGCAGCAACAACTGCTGAATCAATTCCTCCAGATAAGCCAAAAATAACACCTTTAGCTTTAGATTTTTTTACATAATCAAACAGCCAGTTTTGGAGTTCTTTTAAATATTTAATTAAATTAGTCATTAATTACAAATTCACAACCATCAAAGAAAATAATATCACCTTTTTTAGCACCAACGGATTTTAATTGATCTTCAATACCGATTTCTTTAATTTTATTTCCTAAGCGACGAATGTTATCAAGCGTAGTTTGTGGAATTTTATCAAATCAATATCTTAGACGTTTACAATTAACAATTCATCTGTTTAAACTATCTTTTTCAATATTTAAAGGGTCGTTATTATTTTCATTGGTATTTTGAAAAACATAATGCTTCTCAAGTTCCATTGGCAAACTAAACTTATTAGCTCCAAATTGGGAAATTGTTTTATTGTAAAGTTCAAAAACTCTATCAAGTAAATTGCCTAATTCTTTCTTTAAAGCAGAAATTTTTAATACAGAAATAGATTTTTTTTGAAGAAATTTTTCTAATTTTTTAAACCGTTTCTCACCTTCATTAACATCAATTTTATTAGCTACAACAAGCATCTTTTTTTTAACTAGAAGGGGAGAATATTTTGAAAGTTCATCCATTATTTGTAAATATGCTTTGCAAGGATCATCATTATCTACAGGATCAAGTGAAATTAAATGAATTAATATTTCACACCTCTCTATATGCCGTAAAAAATCATGTCCTAACCCACTTCCTTCACTAGCATTTTCAATCAAACCAGGAATATCAGCAAAAACTAAACTATTATTTTGATATTTAACTACACCAAGTACGGGTATTAAAGTAGTAAAGCGATAGTTTGCAATTTTAGGTTTGGCATTTGAAAGTTTGGAGATTAGTGTTGATTTACCTGAATTTGGAAAACCAACAATTCCTACATTAGCTAAATATTTAACTTCCAAACTAACGTTTAAAATCTCTCCTTTATCACCATTTTCATATAGATTAGGAGCACGCATAATTGGGCTTTTAAAAGCAGCATTTCCTTTTCCACCTTTTCCGCCAAAACACAAAATAAAACTCTGTTTATCATGAACAAAATCCACCAATACACTATTATTTTCTAAGTTTTTAACTGTTGTTCCTATTGGCACTTTAATTAAAAGATCTGAACCATTTTTACCATGTGCTAAATCTGGTTTACCATTTTGTCCATCTTCTGCAAACAGGTGTTTTTTATTCTTCAAGAAAAAAAGTGAATCGCAGTTATGATCAGCTTGCAAAATAACATTACCACCATTACCACCATTACCACCACCAGGACCACCTTTATCATAATGTGCTTCTCTTTTTCATGCTATGATTCCATTACCACCATTTCCAGCAGTAAAGCGACACTCACAATAATCTGTAATAGCCATTATTTTGGTTTCTTTTTCTTTATTTTAAAAACGATTTCTGCAGATTTTTTTCTTTCTAGATCACTTTGAATTTGTTGCAACTTTTGTCGATTTTTAGTTTTAGATTTACCTGTCTTTAAGAGATTTTCAGCAATTATTATTGCATATTTTGATCTGATTAAAAAAAAGTTAGTCATTTTATTCAAAAGAAAAAACTTAGCATTAATGTTATTAGAACTAAAAAAAATCGAAGTAAGATAATTCAACTATTTCAAGCCATTGGTATTATTCCTGATAAAAAAAGAACTATTAAAGTAAAAGCAGTAGAATAGCAAAAAGGAATTAATTCCCAAATAATGTATTTAGTAAAAGCAAATAAAAAAAAGGCTAAAAAAAGATAAATTAGTAAAGGAACAAATAAAAATCACAACAAAACTAAGTCAAAAATGGGCTCTATTTCCTTACCCATAAATGTTCTGTTTTGTGCTGCAATTAAAAAATTAAAGCCTAGTAAATTGATTTCACCAAAAAGAATTCAAATCAAAAGATAATAAATAAAAACTAAAAAGAAACTAAAACTTCCTATTAGTCAATTAGGGGTTTTAGTTTTTTTATTTGACTTGAAAAAATAATTCAAAACTAATAAATTTGTGCAGTAATTTGAAATTTCACAAAGCGATCGTTTTCATAAAATTGATAACGAAGAAGACCCTTGATAACTCAAAGAATAACTGGTAATCCCAGGTTTTGAATTTCTTTGCAGTTCTTATGATAAAAACTCTCGTTTGGCACTAAAAAACGGCAATATCTCATTAATTCGTCTCTAGAAGCTACTAATAAAGCTTTTTTGTTTGCAATTACAAAACCGGTTTTAAAGCCTTTGAAAGTCTCATTTATTAACTTAATGATATGAAAATTACTACTTAACAAAATCATTTTTTCTTTAGCTAAGACAAAATCTTTGCAAAGCAAATTCAACTGTTCTAATAATGAATTGTTATTTTCTTTATCATCTTTAATATGAATCAGCAAATATTTAAAGGGCTGAATAAATTGCGTTAAAACTTCCTTTAAAGTTTGAATTTGTTGTGAAGTTACCTTAAGCTTAAATTCACTTGCAAGGTTAACCTTTTTAAGATCAACTAAATTAATTTGGTTTAAATTCAAGTTTTTATTAGAAACTTTAAAGTTTTCTTTATGAGTTACTACTATTTGTTTATCTTTGGTTAATTTAATATCAATTCAAATACCATCAAAATCAAAAACTTGGGCTGCTTGAAATGCTAATTTGGTGTTTTCTGGTGCAATAGAACTATAACCGCGATAAGCTATTAAAAACTGTTTTCGCATTACTATTTTTTATCATTACTGCCAAAAACATCCTTAGCATCATCTGCTGAAAATTGACTAAGTCATTCATCTGCGTCAATAGAGTCAATATCATAACCTTCAAGAGCAGGAATATCCTTAGTTATATCTTCATTTAAATTCTCAGTTTCCTCAGACTGGAATACTAATCAGTTACCTTCACTATCAAATTCCCCATCATATCAGTTTCATTCATTGTCTTCATAGTAACCATATTCTTCATTTCCGATTAATTTCTCTCAAAACTTAGGTGCCTCGTTAATTCGTGGTTGACGATTCTTTCATTGTTCATCAACAAGAACTCAGTCTCCAGATTCATCATAGAAACCATATCAAATCCACTCACCTTTATTGTTGTACCTACCATAAACTTCATCACCAATAAGTGGTAAATAATCTCTTTCTCTAGCTCATTTAACAGTTGCATCCTTATTTCATTTTTGATCATCACCAAAGAATCCTGCTCAAACTCATTCTCCATCAGGTTCATAATGACCATAACTATTGTTACCAACTAATAATTCTCAATCAAGTGATTCTTCTTCACTAGCTTCTGGTTGAATTTCATCAGCTTGAGGTTCTTCCAATACTGTTTCAGGTTTTCGTTCTTCAAAGTTAGGTTGAACAACTACTTCAGGTTCACTAGAAACTTGAGGTTCTTGAATAGTTTCAACTGGTGAATCAAATTTAGCTTCTTGAGGAGTTTGTTCAGGTTGAACAGTATCAAAAGTAGCTTCTGGTTGAGATTCTACAGGTTGAATTTCTGGTTGAGTTTGAACTTCTTCAAGGAGAGGTTCAGGTTGTGAATCTTGAACCGATTCAACTGGTGAATCAAATTTAGCTTCTGCAGATGGTTCAAAAACTGCTTCTCCAGGTTGTTGTTCTACTTGAGGTTCAAATTGTGGCTCTGATGGTTTTGATTCAATTTCATAATTTGGTTCATCAAAGTCATAATTAGGTTCATCTACATCATATTGAGGTTCTGATGGTTGTAGATCTGAATCATAACTAGGTTGATCAAAATCATAGTTAGGTTCATCAAAGTCATAATTAGGTTCATCAAAACTAGCTTCTGATTCAACATGTTGTTGAGTTGGTTCACTACTAAATTCAGGTTGTTCAACAGTTTCAACTGGTGAATCAAATTTAGCTTCTTGAGGAGTTTGTTCAGGTTGAACAGTATCAAAGGTAGCTTCTGGTTGAGATTCTACAGGTTGAATTTCTGGTTGAGTTTGAACTTCTTCAAGGACAGGTTCAGGTTGTGAATCTTGAACCGATTCAACTGGTGAATCAAATTTAGCTTCTGCAGATGGTTCAAAAACTGCTTCTCCAGGTTGTTGTTCTACTTGAGGTTCAGAAATAACTTTTTGTTGATTTTCAACATCATCAAACTTAACTTCAGTAGTTTTTTCAGGTTGAACATCAAAAACTGCTTCAGGTTCTCCAACAGTATCAGTAGTTGTTTGATCAACTACTTCAGCTTCACTAGAAACTTGAGGTTCTTCCAATCCCTCAGTTTGAGTTTGATTTTTATCAAAAACTGCTTCATGTTTAACAGTATCAAAGCTAGCTTCAGTTATTAATTGATCAACTGGTTCAGATTTAGGTTCCTCTTCTAATATTTTTTCAGTCTGTGTTTCTAATTGAGGTTTTTCAAAAACTGCTTCTGATTCAGCTTCAGATACATTATCAAAAGTAGCTTCAGATTTTAATTCTTCTGTTGATAATGGTTCAGAGGAGGTTTCCTGTTGAAGTTCTTTAGTTTCATCAAAAGTAATTTCAGCTTCTGTTTCTTTAGGTTGAACTTCATTATTTTCAACATTATCAAAGCTAGCTTCCTCTAGTTTTAAATCTTCTAACTTGGTTCCATCAAAGATTTGTTCAACTTCAGCAGTAACAATTTCTGGATGATTTTGATCTAATGCAGGTTCAGATGGTTGATCTTCAGAGCTAGGTTCTAATTGGGTTTCAATTGTTTCTGGTTCAAAAGCAGTAACTTCAGTAGGTGTAACAACTGTTTCTTCAGACTGATTTTCTAATTGTTGATGATCAAAAACTGCTTCAGTTTTTTCTTCTAATTTAATTTCATCAGAGATTTGTTCAGCATGAAATTCAGCTTGCGATGATTCTTGGGGAATTTCGACTGGTGAATCAAATTTAGCTTCTTGAGGAGTTTGTTCAGGTTGAACATCTGGTTGAAAATGATCATCAAAAACAACTTCAGTAGTAGCTTCAGGCTGTAGTTCTTGAGTTTTAATTTCATCAAAAACTGCTTCTGGTTGAACAGTATCAAAAGTAGCTTCAACTTGTGATGATTCTTGAGGAACTTCAACAGTTGAATCTAATTTAATTTCTTTGAAAGTAGGTTGGAGTTCTGATTCAGAACTCTCTTTTTCGTCTGGTTTTGAATCAAATAAAGCATTTGATTCAATGATTTCAGTTAAATCAAGCGGTAGAGGAGTGAATAAGTTATTTTCATCTTGATGTTCAAATAATTTTGTTTCTTGTTCAACTGTGGGTACATTTGATTCAGAAGTCTCAACCTCTTGAGTTTGACTAATGAATTCAAGCGTATCTTTTTGTTCAGATGAAAATTCATTAGGAGTTTCAGCACTTTCAGTGAAACTGTCTTCAACTATTTGATATGTTGGTAATACGAACTCACTATCAGTTTCTAGTTGTGTTGTTGGTTGTTCTTGAACTTCTTTTTCAGAAACATCTAAATTAATAAATTCATTACCAATTTCTTCATGATTTAATTCAACAGATGTAGTTTTAGTTTCTAATGATTTATCAACATCATTTACAGAATCTAATTCAAGTGATAGGTCAGGTTGAATGAAAGGTGGTTCACTTTCTACAACTATTGTTGTTTTTTCATCAAATAATGATTTTGGTTCAGTTTCAAGGAAATCTACTTTATATTCCAATCCTTCTTGAGGTTTTGGATCATTATTAATAAAAGTTATTTCATCAACTAGTTCACTTTTGGGAAGTTCACTTGAAACCTTAACTTCTGTTTCTGTTGAAAAAGGAGCATTAACATCAACATATTCATTTGCTAAGAGTACCTCATTAACAAGTTTTTCACTAAATAAAGCAGTTAAATCAGTATTAACACTTTCATCACTGTTTGTTTCTTTTATCTTTTCATCTGAAACAACAGGTTCGCTAAATTCTTGTTGAAATTCATTAACAGAATAAGTAGGTTCAAATTGTTGTTCTGAAACTGTTTCATTTATTTGAGAATCAATATTATTAACAACTTCATCTCTAAAAAGGGATTCATTAATGAAATCATTATTAGCATCTAAAATAATTTCATCTACTTGTGATTTTTCTTCTACACTTTTTTGTGTTTCATCAGTAAAAGAACCGAATTCTTTTCAAACTGAATCAATATCATCAGAAATATTAGATAAATCTGCTTTAGCACTATCTTTTATTTCTTTAGAATAATCTTGACTTAAATGTTCAACATGTTTAGCAATTTCATCATGTTGTTGGTCGTCTTTAATAATAGAATCTAAGCTTTTAAGATCATCTTCGTAAACAAAAACGGTCTCAACTTCTGGAACATTTCCTGCTTGAGTTTGATTGGAATCTAAGCCCTTAAGATCATCTTCGTAAACAAAAACAGTCTCAACTTCTGGAATAGTAGTTTCAGGTTCACAAGCTGCTTCAGGTTCAATTTCAACAGGAGCCTCAACTTCAGATTCAACCTTAACTTCCTCTTTAACGATTTCAATTAAAGGGATGGTTTCATTAGTTTCTTTAACTGGATTTAAGAATTCAAATTTTTCTTTTTCAAAATGTAATTTAGTTACTGGATTACGGAAGATAACTTCATTTCTTCTTGGTTTAACTTCTTCAATTACCGGCTGCTCTTCACTTGAATTTTCAAGTAATAGTTCGTTTTCTAGAGAAGAAGCTAAGCTCTTGGTATTAAATACTGTAACAACAATGTCATTTTCCTGTTCTCAGTATGGTTGTTCAGAAATAGTCTTTTTCTCTATTTCATCAACTAAGCTATCAGTATGACCAAGTGCTTCTTCAGTAAAGCGAACAGGATCAGGGATCCAATTTAGTTTCTTGTCAAAAAATCCTTTTCAGATCCATGTATTATTTTGAACAAAACCATAGTTAGGGTTGCCTACAAGTGGTCTTCAAAAATCAATATCAGCTGGTCTTTGTTTAATGAAAGCTGCATGTTCTTGAGGAACTTCAACTGTTTCAATTACCTTAAAGTAGTTTCATCTTTGTAATTGGTCAAAATAACCTAGTCAAACTCAAGTATGATTTTTTCAAAAACCATATTTTCTATCACCAACAAAGATATATCAGAACGCTTGTGAATCTTCTGGATTTAATTTTTTTTCTCTAATAGTTGTTGTAAAACTATCTTTTTTTGTTTCTAGAACTTTGGTTTCTGGTTGATTGTTTAATTCAACCAACTTATTTTCATTAATGGTTGGATAACTGCTTGTTTGATTTGGAACTGTATTAACAACAGTTTTTTGATTAAGTTCTCTTCTACTTAAGATATCTGTTTCAGGTGGAACAATAAAAACACCGCGCTTGGTTTCATCAAAAAAATCTATAGGTGATTTGCTCTTTGATTTAACTGTTTCCTTTTTCTTACTATTAAAAGACTTTGTTTTCTTGAGAATGTTTTTTTTGATTTCACCTAAAAAAGGTTGATCAAAAAGATTTTCAACTTTATCTTGTTCTTTTTTTGGTTTAGTAACACTCTTCTTGTTAGCTGATTCTAAATACTTTTTGGTTTTTGATTTAGGGGTGGTGTTTTTGTTTTTATTCTTTGTTGTTTTTGGCATTAATAAAAATACTTAGCACTAGTGACTAAAGTTATCTGACTCATTAAATTGTAATTTTTAATATCAAGATAACAAAAGTTAGCATACATGCTAAAAAATAAATAGATTTTAATTACTATTTATTTAATTTATTTAATGTAGATTTAATAGTGTTTCAGCTTCTTTTTGCAAAAGAAAAATAGTTATAAAATCAAAAATTAATCACTTTAATTTCAAAAAAATTTGACAGCTAAAGCTTTACATTTTATTAACAACAGAACAAATAATTTTTAGCTTTTAAAAATTTATTTAGAAAGAAAACTATAAACAGGCAATTTTTGTTTTTCTGTTTTTACAAAAATATCAGTAAAAATATCACAGTCAAAATGATTTAATAGTTTTTTGCGAGTTGCTATCCCAATTGCTTTGATCATTTCTGCATTTTTTCCAATGATAATCTTTTTTTGGCTTAATTTTGGAACACTAATTACTAGGTGAATTTTTAACAAGTTTTTTTCTTTATTGAAACTCTTTTCAATAATTTCAATTCTTGCTATATGGGGAATTTCGTTTTTGCAATAAAAAATTATTTGTTCACGTAATCCTTCAAGAATCTTAAAGTCATCGTTGTTTGCATCAATAAAATTGATATCTTTACGAAAGATATTATACCTAAGTTCAACTTGTTTAAAAATTGATCAAAAGAGGTTTTTATCAAACTTAAGTAAGTTTATTTCAATTGTTTTTTGTGGTTTAAACTCTTCTAAAATAATTGCTTTATTAACTTCACTTAAACTTTTTTGATGAAATTTATTAATTAAAAAAATTCTTGTTAAATTTTGATAGCGCTTTAATTGTTGTAATTGAGTTTTTAAAAATTCTATCTTGTTATTCTGATCACTTCTAACAACTAAAAGCAATACATCAATTCCACTTAATGCTTTGCGAATTTCTTTAGTTATTAATTCATAATTTGAATGTTTTTTCTCAGTAAAACCAGGGACATCAATAAAAACAATATTTTTATTAGCTTGGTTAATTACCTCAGTGGAAATAGAAAGAAGCGTTGTGTTATTCATGGATGATACCATCAGACTATCATCGTTATGTAAAAAATTAATTAGCGTTGATTTTCCTGCATTAGTAGGTCCTAAAACACCAACTTTTAAAACTTTCATACTGATAAGATCTCTTCATGAATTGCATCTTGAAAAAACATAGTAACGCTTTCAAATGCTTCACTTTCTTCATGATTAAATTCAAAGAGGTGTAAAATGCCGTGTATTAAGCATCTAGTTAAAAGAAATCAATTGTTACAACCAATCTCTTTAGCTTTTTTAAAGATGTATTTAGGACACAAAAAAATTTCGCCGATTGCTAGTGAAAAACCGGTTTCAGTTTCGTTATAGCACAAACTAATTACATCAGTACAACCTCTACGTTTTAAAAACTGTTTGTTCAGTTTTTTCATTTTTGAACAGGAAACAATTAAAAGCGCAAATTCTGGGTCAAACGATAGGTTTAAACTGCTTTTAATAATTTGGAAACAAAGTTCGACTCCTGCTTGGTAATTTTTACCAAAAAAGCGCTTAAAAAGGCGGCTTGAATTAATGCTAAAAGATGACTTCAAACAAATCTTTTGATGGTAACATGATAGTAATATTATATCAGCCCTGTTCTAGAGTAACACTTAAATCTACCTTTACTAGATTTTTGTTCAATTCTGGTGAAATTTCTGATGTAGGAATTTTCTTTTTGTAAAGTCTAAAAGCTTTTTTTGCTGATTTTTCTGTTGTTTCTGCTTGAAAATTAGTCCATGAAACGTTGTTAAAAAGATTTTCATCTTTTTCATTTTTAAACATTAAAAAATTTTGTTGTTGCGTAGTTCAAAGATTTACATTAAGATATTGTTTTACCTTTACTGAAAACAAAATAGCAACTGTTGTTAAAAGCAAAATAATAAAAGCAATTGTTATTCCTAAAATTTTCTGCTTCATAATTTAACTATTTCTTAAGTAATTAGTTAGGTTAATCTTGTTTTGAGCTTCACTTGCAAAACTCAGATCATGCTCAACTAAGACTACAAAATTATTTAAAGTAAGTGGTTTTACTAATAGCTGATAAACACGGTTTTTAATTTCAGGTGCTATTGCATTCATGCATTCATCAAGTAAGATCACCTTATTCTTAATGTTGCTAAGTAAGAATAGTTGTAAAATTTGTTTTTGTCCTGAACTTAACTTTGTTTGATTTTGAACACCTGCATCAAAAAGTAACTGATAGATATGTGGATTCTGATTATTAAATAAAGCCTCACTAAAGCCAAAATCATTTGCTTCTGTTTGCATTAAATTTTGACCCTTTAGATAATAAACGTTTTCAAAAAGCTGTTCTTTTGAATATCTTTTTAAATCGACGTTATTAAATTTAATATTGCCCGTATATTCAAAACCTCTACCAGTTAATATTTTTAAAAAAGTAGATTTACCACTACCATTTTGTCCAAAAATAACTGTTTCATTTTTGATTTTTAAACTAAGGTTAGCTAAATCAACGTGTTGATTAAGTGTAACTGATAAATTTTCTAGACTAATTTCATCAGGACACTGTCAATTTACTTTAATACCCTCATCCTTTTTGGTTTCTATAAACAAGTTAACTAATTTCTCACGACAATAGTGATAAATGTTTATTGCAGCACCAAATTGAACAATTCTTGTAGCATAAGTTGAAAAAAGTGATTGGATACCAAAAACATAGAATAAAAAGCTCAATTGATAACGCTGTTCAATAATACCAATCACCCCCAAACCTAATATAGCAAAATCAAAACCTTGTTGGAAGAAGCTTTTAATGTTTTCTATAGTTAGACGATTAATTTCCTTCTGGTTGTTAATGTTTTGATTTTGTAAAAGTGCTTTACGAAAATTCAACATTAAGAAATTAAAGCGCTTTTTATTTTGTTCTTCACGCAAATTTTCATCTAGCTGTAAACTAATTTTGTTTTGCAATTCAACATAAGGAATTTCCTTCTTTGTAATTTTTTTAGTAAAAAAGAAGTCATAACAAAATAAGCCAGCATTGACTACTATTTGTGCAATGGCAATCAATAAAAATTCTAATTGATTAATTCCTATTAAAACACCGATAATTAGTGCAACAACACAACTAATAATTAAATTGGGAATATAAAAGTTAAAAAAGGAAAGAACTTCTTTTAAATAAAATTGTCGTTCTATTAATTGATTTGGTGATTTATTACTAAAGTAAACAAAGCTTTTTTGTTGCAAACTTTGAATAATTCAACCCAAATATCCACGGTATTGTTTTCAAAATAAAAAATTCCTAATTAGTTGCAAAATAACTTGCAAAAGCAAGTTTAATCCCTTTAGACATGAAAAATAAACTACCAAAACAACTAAATTAACTGCAGTTCCAAAATCAACAGTATTAGTTATTATGGTACGCGATGCTGTTGCTAATAATGTAGAGATACCAATAATAATCAGTTCAATAAAAACATAAAAAGTGACTAAATTAAAATCAAAAAAGTTACTGAAAAAATTACTTTTATTTACTGGTGGAATCTCTTTAAATGCTTTTGCAACAGTTGCTGCATAACCCGTTCAGAGTTTTTCTAGCTTTTCATCAGTTAAAAGGTATTTTCCTTTTGCAGGATCATATACCTCTCAACTATTTTCATGTTTGTTTTTAACTATGACAAAGTGATCTTTAAAATGAACTATTATGAATTTGCTATCAAGTTCTTTTAGTTCTTGAAATGTTAATTGATAACTGTTGATTTCAAGACCAAACTTTTTGCCATAACTTTCCATTTCAAATAAGCTTAAGCCATTTGGTGGTAAGTTAATTTGTTCTAGTAATTCATCATGAACATACTTTTCATCATGGATTGCATTGGCAAGCATACCAATTACGCAAATTCCACACTCATTCTGTTGTTCTTGATAAATGATTTTCATCGCCTTTGTTTTGTCTTATTAATAGCAATGAAAAGTGAAAAATGACAAATTTGTAAGCTGTTACAAACACTACAAACTGTTTTATTAGTTAAAAATAAAGTTACTTATTATTTATTTGCAAGCTGACTAGCACGTTCGTACAAGGTTCTCACTAAAACACCTTGTCCAAGGTTCTCAATGGAAGCAGTTGATGCAATATCACAATGGATCAAAGATACACCTTCTCTAAATTCTGCAAGGAAACAAGCTGCTCTTGAAGATCCAGCACCTCTTGCACTAGTAGAATTTTGCAAATCAGCAAGTTTTGTTAGCTGTAAAGGTTTTAAATAATCAGGGTGCATAGGTAATCTTCATACAGGTTCACCTGCACTACATGCTGCTTTTTTAAAAGATTCTCACTGGTGATCACAAGTACTGAAAATACCTGTATAGGTAGTACTCAATATGTATGACATTAAACCAGTAAGGGTTGCTACATCAATAATATGTGTAGCAGCTAAATCCTTAGCAGCATATGTAATAGCATCAGCTAAAACCAATCTTCCTTCAGCATCAGTGTTATCTATTTCAACACTTTTACCATTGTATGCTATCTTAATGTCATCAGGACGTTGCGCCTTAGCACCAGGAAGATTTTCAGTAAGAGCTGCTACTGCAACAACATTGGTTTTAACCTTGTTTTTAGCTAATGCCAAAACAGTAGAACATACTATAGCCGCACCACTCATGTCATATTTCATGCCACGCATATAATCACCTGTTTTAATGTTCATTCCGCCTGAATCATAAGTAATTCCTTTCCCTACAAAAGCAAGTTTTTCTTTGGAATTTTTATTAGCTTGATAACTAATAACAAGTAAACGTGCTTCTCTTTCAGAGCCTTGATTAACCCCTAAAAGTAAACCCATTTTTTTCTTAATTAGATCTGATTGTTTAAGAACTTTTATTTTTACAGGCAATTTACTTGCAGCCTTTTCAAAATGTTTAACAAATACTTCTGGATAAAGTAGATCTGAAGGAGTGTCTTGTAATTCTCTTGCTAGGTTAACATATTCGCCTACTGTTTCATATTCTTTAACTAAGTTGTGATATTCAACAGCACATTGAACAGCAACTTCAACATTTTTTTCAGGTTTAGTTTTCATTGTGAAAGGAGTAGTTTCACAAAAAGTAACACTTGTAATAACAGTTTTTATCGCAGTATGAAAACAACCACTATTTTCATTTTTCTTATAAAGTTCTATAAGACTGTCAAGATTAATGTTTAATTTGGTTTTCTTTCTTTTAAGAAATTGAACTAAAGCACGGTTGAAAGCAGGAAAATCAGTTGGTAAAACAACACCAAATTCAACATTAGGATTATGTGCAGCTTCTTCTTTTACACCATATGTTTTTGAACTCACAAAAACAACTGTGTTGCTATCATCACTAAAAGGTTTATTTATTCTCATTCTATATCTCTATTTATTATTCTGAAAAGGAGTTATCTTTTCTCTCTTTAACATCATACACAGCAACATTAGTAGTAATTAATGAACTAGCTACAGAAGCAGCTTTTTCAAGTGCAGTTTTAGTTACTTTGGTGGGATCAATGATTCCATTTGCAATCATATCAACCTTCTTTTTAGTCTCAGCATCAAAGCCAACACCAGTTTTTTCATTTTTCAATTCACTGAGAATTTTAACTGGGTCAACTCCTGAGTTTTGAATAATTTGACGCGCTGGTGCTTCTAGAGACTTTTGCACAATTTCAAAACCAAGTAGGATTTCTTTAATGTTTTCAACACTAGTTTCATTTTCATATCGTTCTTTTAGTTTACTGTTAGTTAAAACACAAGAAGCATTTAATAAACCAACACCACCTCCAGCGATAATCCCTTCTTCAACTGCAGCTTTGGTGGAATTTAAAGCGTCTTCAATTCTGAGTTTTAATTCTTTTTGTGCGAGTTCAGTTGCACCACCAACACGGATAACAGCAACACCTTGACTTAAATAAGCAATTCTTTCTTTAATTAAATCAGAATCATACTTGTCAGTGGTTTGTTTTAATCTTCCGTTTAGAAGTTCAACATGCTTTTTGATTTTATCCTTATTGCCTTTACCACCAATAACAGTAGTTTTTTCTTTAGCTATTTGAACCTTTCTAGCATCACCTAAATTATCAACAGTAACATCTTTAAAACCACTATTAATTTCGTTATTATATGCTAAGGTTCCACTACTAATTGCTAAATCTTCTAAAGCTGCTTTTTGACGTTCACCATATTCATTACATTTAACAGCAACAACATTAATGGTGCCCCTGAGTTTATTAACTGCTAAAGTAGTAACAACTTCTTCTGCAAAGTCAGGTGCAACAATTAATAATGGATTACCATTTTCAACACTACCTTCTAAAAGCGGAAGAATTTCTTTAATTGTGTTAATTTTTAAAGAGCTTACTAAGATTTTAGGTTGTTCTAAAACAACTTCCATTTTTTCTTGATCACTAACCATATAAGGTGATGCATATGTTCCTTTAAATTCAATTCCTTCAGTGGTTTCTAATGTTGTATTAATGGTTTTTGCATCATCAGTTGTTATCACGCCATTTTTACCAACTAAAGCCATTGCTTGAGCGATCAGTTTACCAATTTCTTTAGAACCTGAAGAGATAGCTGCAACTTGTTCTATCTCTTCGTTAGTATTAATTTTTTTAGAGTACTTTTCAAGTTCTTTAATAATAAGTAAGCTTGCATCTTCAATACCCCTGCGGATGTTAACAGGATTAGCACCTTTATTGATAATTTCAATACCACGGTTTGTCATTTCTTGTGCTAATATGGTAGCTGTTGTTGTACCATCCCCAGCAATGTCATTAGTTGACACTGCAGCAACTGAAATAACCTTAGCACCAATATTTTCAACTGGATCACTAAGTTCTATTTCTTTTGCGATTGTAACCCCATCGTTAGTAATTAATGGATTTGCAAATTTTCTCTCTAAAATAACATTTTGGCCTTTAGGACCTACTGTTACTTTAACAGCATTTGCTATCTTATTAATACCCTGCAACAAGCGGGTTCTCGCATCTTTACCAAAGATTAATTCCTTTGCCATAATTGTTTAATTTTATCTTTTTCTTTGCTTACCACTTTCTGGTTTTTCAAAGGCAAGTACATCCTCAAATCCAATAATTTTGTACTTGTTTCCCTCATTCTCAAAAGAGATACCACTATACTCCTTAAAGTAAATAATATCACCAACACCAAAAGCATATTTTGGTTTTTCTGTTTTGCCATATGCAGGACCAGCACCAAGAGCAATTACAATCCCTTTATTAGCATTAGCATCGCTTTTATCATTACTTGCCAATGAGGTAATAATCCCTTTTTTTGAGACTTCTTCTTTGTTTGATTCCACAAGTGAAACCAAGACGTTGTCATGAATTGGCGTTATGTTCATAATAAAAATATTTTAATAATTAAAAACTAATTTAAAAGAAAGTAAAAGTTATCTTAAATTTATCTGATATTAGCGCTAAACATTAATTAATATTTAGTGAGATATTGTTAATTTTTAAATACAAATTAATAAGCTAAACCCTTATAGAGCGGAAAGCGTTTTAAGAGATTTAAAACAGCTGTTTTTGTTTGACTAATTACCTTTTGATTACCATTACTTTTAATAACCTTATCAATCAAACTGGCAACAAAAATAAAGTCATTAGTTTTAAAACCTCTGGTTGTCATTGCAGGAGTTCCAAGTCTAATACCTGAAGGACTAAAAGCAGATTTTGTTTCAAAAGGGATTGTATTCATATTCAAAACAATGTTAGCTTTTTGTAACCACAACGCAACATCTTTACCATTACCAACCACTAATGAAAATAAGTGGGTTTCAGTACCTTTTGACACAACACGATAACCCTGCTTTAAAAATCAATTTGCCATTGCTAAAGCATTATCTTTAACTTGTTGCATATACTGCTTAAACTTTGGATTCAAAGCTTCTTTAAAACAAACATATTTAGCTGCTATCACATGTTGTAAAGGTCCACCCTGACATCCAGGAAATACTCCACTATCAAGCTTTTTGATAATTGCTTGGTTGTTAGACATAATGATACCCCCCCTAGGACCACGCAAAGTTTTATGAGTTGTTGAAGTGACAACATCCACAAAAGGCAAAGGGTTTTGGTGCAAACCTGCAGCGATGAAACCAGCAATATGGGCAATATCAGCTAAAAGATACGCATTAACTTGTTTTGCAATTGCACTAAATTTTTTAAAGTCAACAGTCCTAGAATAGTTAGAAAAACCACAAATAATTAACTTTGGTTTGTGTTCGAGAGCAATTTGAAGAATTGCATCATAATCAAGAGTTTCTGTTTCAAAATCTAACGAATAAGTTACTGCTTGATATTGCTTACCTGAAAAATTAACGGGGCTACCATGGGTTAAATGACCACCACAATTAAGATCTAATCCTAAGATAGTATCTCCTGGTTTTAATAATGCTAAGTAAACTGCATAGTTAGCAGATGATCCAGAATGAGGTTGAACATTAGCCCATTGTGCTCCAAATAAAGTTTTGCAACTTTCAATGGCTAAGTTTTCAGATTCATCAACAACTTCACAGCCTCGATAAAAACGTTTACTGGGATAGCCTTCTGCATATTTATTTGTTAATACTGAACCAGTTACAGCTAATATGTCTTGGCTAACATAATTTTCTGAAGCGATTAAACAAATGTTTTCTCTTTGACGTTGTAACTCTTTATTAAGTAAAAATCTTACCTTTGAAAACATTCAACTTAACTAACGTAATAAAAACGGTGTTTTTTGATTGTTCTTATCAACATATTTAAATTCTTCCTTTTCACTATTTTTAATTTGGTCGTACAAGTGGGTATTGTGCTTTTTAGCAAATTGTGCATAGAAAGATTTTGTGTTACTGTCACCAAAAATTAAATCATAGTGCTGAAATTTATCCCTATTATTAGTAATTCTGGTTTTATTTTTTTCACTTTCACGTTTAGAAGTTCTTAAAAGTTGAGACATACCAATGTTATCTTTATGTGTTTGGGAATTCTTATATACCTCTAAAACACCGAAATAAATACTGTTAATTTTTTGGTTGTTGTTAAATATAGCACTTCCAGAAGAACCACCAGGGAGATTGGTATCATCAAGCATTAAACCATATCCATACTGTTGGTACTCTTTGTCATGATATTTAAGTTTAAAGTTTTTGAAGTTTTGAAATGTAACAATCCCTTTTTCGTGTTTAATAACTTTATCAACATGACTGTCTTGGGTTTTTTTGGAAAATATTTCACTACCTATTTGACCATCTTTTCTGGCATTTTTTGTAATTACAGGGATCTCTTTAGCAGTTGAAACTAAATATGTGTTTTTACTCCATAACTGACTTTTTTGATTAAAGTTATGAGTTCTAGTAAACTCTTCTTTTTGTTCTAATAAGCGGTTTTGATCATAACCTCTTTCAAGTACAGGATAACCCAAAAGATAGTAGCTATGGTTCCAATGTTGTTCTAAATCTTTTGTTTCAAATATATTAGTACTATCACCCAAACTTTTATAAGTATTAATTGCTGGCTTAATGAATTCATTGAAGATTTGTCAATCACGAAGATTTTCTAAATTAAGTTCCAGTTCTATAACAGCAAAGTCTGCATAAACAGGCTTAATACTGTTAACACCTTTCAAGAAATTAGTTGCGGTATATGCAGTTTTAGGAATATTTGATGTTTGAATAGAAAGCAGTGAATCATTTACCTTTCTTTGTTCTAATTTTTCTTCTTGATAGAGTTTTGTAATATCTGTTTTGTTAGTGTATTTACCAATTGAAAAATAAACAGTTGATTCATATTCATTAAACTCCCTTTGATCACCTGTTTTGCTGTAAGGTCAATGATCACCGATATTTCTCAACCCATCAGGAACATGCAAATTAGTTGCAATGTAAGCGAAAAAAGAACCTTGCTTTTGGGTTGTGTTTTCATCACCTTTTCAGTCAATTAATCAACCAGTGCCATAAGAGTCATTACTAAAGCTAGTGAAATTTAAAGTAAAAGTTAAATCATATCCTTTTCTGTATATATCACTTTCTTCTGGGATAAAACTTGTATTAAATTTAAAGTTTCTGTTATAAACCTTCTGTTCTACCTTAGGTTGCTGATTTACTTCTGTTCCTTGATCAGATTGTTCATCTTTAAAAGGAGTAGCGTTTTTATCTGTTGTGCTGTCGTTTTTTTCTATAGGAACAGGAACAGGTGAAACAGCAGAACAAGAAGCTAAAAGTACTTGTAACGAAATTAGTGAAAAACTTAAACTTTTTAATTTAAACCACATATCAATCTTTAACATTTTAATACAAAAATATTTTCAAAACCTTTGAATTGATGAAGTTAAGAAGCACTTGTTAAACTTAGCTTTTAGCTTCAAAAATAATTGAGTTCATCTTTTCACTGATAAATTTAATTATTTTTATTCTGCTTAGTTTTAGATGCAATTAAAAAGAAAAACTAATGAAAAAACTAATGAAAGCAAATGCAACCATCAAAAAAGTTAAAAATGAAAATAGAACTACTATTGACTCAGGTAAACTAACTAAATTAAAACCAAAAAAATTAATCCCGTTAGAATTTGCATTGGCTTGATAACTACTATCTAAAGCTAGAAAGCCAACTACAAAGCTACCAATTAGAAGAATTAAACCAAAAATAAATCAAAAAACACTATTCAGTTTCTTTTTCATATCGAATAATCTTATCTATCCGTCTTTGGTGTCTACCACCTTCAAAATTAGCTTTGAGAAAATCATCCACTATTTTAATGTTTTCACTGTCAGTTACAAAACGACTGGAAAGACATAAAACATTGGCATTATCATGTTGTCTAGCTAAAGCTGCTGTTTTCTCACTAACAACCAAAGCCGCTAATACTCCTTTTACTTTATTTGCTGCCATACAAACCCCAACACCAGTACCGCAAATTAGTATCCCAAGATCTTTATCAGAATTTTTCTTGACCTTATCAGCTACCAAAAAAGCAAAGTCAGGATAATCATCATTAGCATCAAAATAATTAGGGCCAAGATCAACTACATTAAACTGCTTAGTCTTTAGGTGTTCACTAATAATTTTTTTAAGAGTTAAACCGGTATGATCAGAAGCAATAAAGATATTAAAACTCATTTAATTCAGTTCCAGATAGTCTTGTTTTGTATTGGTTTTAATTTTAGTTAAAAGATTAATTAATCCTTCTAATGTATCAGGATAGTTTTGTTGATTGTATAGTTTTGTTAACTGTTCTAATGATCTATCGATATGACTACTCTTTTGACGATATTTATTTGCAAAAAGAAAAGCTTGTTTGCACATTAGCCTTAAGTTAACTAAACTAACAACATCACAAATAACTTCAGTCAATTGAATTTGCAATAAATCTATCTTTTCACTAAACAAATTAAAAGTACCTAAATAGTTATTATTAGCTTCAGTGAAGATGTTATCAAGTTCTAATTTCGATTTATTAATTACTTGTAATGTTTTAGCAGGAACCTTTAACTCTTGATCGCTAATTAAATTTTCAATCTGAAAAAATTTAAATCGGAAATCATCTAAAGTATAGACAAAAAAGCGATAGTCATCAAACTTATTAACTGCTAAATTAAGTAAATCAGTTAGTGAATTTTTCAGTTTTTCTAAAGCTTGATTTGTTTGACTCATCAAGTAAAAAAGTTTTTCATAATTGATTTGATTCTGAGCTAAACTTTGATGGTTTAAGTTATTAATATTTTTAGTAATTAGATCGCAGTTCTCATTAAGCTCAATAATTAAATCATGCACATCTGTTTCATTTTGGAACACTAATTCAATGGCTTTAATTGTTGTTTTTAAAAGCTCATTTTTCTTTTCATATTGTTGAATTTGATTACGAAAATCATCAATATTTTCAACCACTAAATTAATGCTTTTTTCTTTGGAACTAACTGCTTGAAAAAATCAACTGATCGCCTCTTGAACTTCATCCATATTCCTTTCGCATAACATTAAATTTAAGTTATTGAGAAAATTGGTACAATCCTTTAATTTAATGGTAATGTTATTAATCAATTCATGAATTTTTAAGAGATCTTGACTGTTAATTACTTTATTCTTCATTAATTCCATTTGCTTTACCTTTTGAATTAAAACCTTAAAGTGATGGGTTAAATAACCTAACTTAATATCAAACATATATTGTCTGTTAGCAGTAATAATTGTTTGACTGAGAATGTTATGTAATTTGTTTAAAAAAGCAATTTGCTTTTCTACATCTTTAGCTTGAATACAATTTTCTATCTCTTTAAACAAGAGCGAAATCCTGTTAAGAACATTTAAATAGAAAAGCGAATCATATTTTTCAATTAAAAAATTATTAATAAATTGGTTTAAAAAACAGAAAGCCTGACGATAAAAAACTAAACAATCATAGCTATTGTTGAAATAAACAATTGCGTTCTTAGTTAGATTAATGTATTCTTGTTGTTTTGTATATAGATGTTGAAGATTTTTATTTAAGAGCTTAATATTTCTATTAAATCAGATGAAACGATAAATATTATTTGGTTTACTGTAAACTGTTAGTTGCTCAAAAGCTGAATTGATTTCATTATCAAATTTGATTTGAAACTGTTTTAAAACTTTAATGGATTGTTCTAACGCTTGTTTTTCATTTCTTAGTAATTGAAATGGACTAATCACACAATTGAAATTGCTTAATTTCAAGTTATTATAGAGTTGTTTGGTTTTTTTTAAAAGTAAGTTATTTTGTCTAACAAAAAGACAAACTAATAGATAAGCACCAAAAATTACTAGTAGTAAGAAAGGGAGCAAGATTAGAAAAACCAGCATTTTATTTTTTCTTACTTGAAAGTTTTAATAATTCAAACATTAAATTTTGTTCAATTTTAACGTTTAGCTTAGCATCATTGATATGATAAATTTTTCTTTCAAGTTCTGTTTTTCTTTTTAAGGTTTCATTAATATCAACTTCATCAGCAAAAACAAAATTTTCTGTGAAAATCTTAGCTTCTGTTTTTTTAATAAGCATTAAACCAGCACCAATAATTGCTTCTCTTTTTGTTTGATCAGCAAAAATAATTTTGCACAGATGGGATTGAATAGCAGCAATCAAAGGAGCGCGATTAAAATTTAATCCTATGTAACCTTCAGTAGTTTTAACTTGCGCACTAATAATGGTTTTATCTAGTTTTATGCCACTAGGACTAAGTACCAAAAAGTGCAATAACTTCATAAAACTACTTAGTTTTATCAAACTCTTGATTTAATCTTTTTGCTTTTTCAACAGCCTCTTGAATGGTTCCAACATAAAAGAATGCTTGTTCAGGCAAATGATCATGCTTACCTTCCAAAATTTCTTTAAAAGATTGAACAGTGTCATTTAAAGATACATATTTACCTGCAATACCTGAAAACTTTTCAGCAACAAAAAAAGGTTGGGATAAAAAGTTACGTATCCTTCTTGCTCTTTCAACAATAATCTTATCTTCATCAGACAATTCATCAATTCCTAGTATGGCAATGATATCTTGTAATTCTGCAAAACGCTGCAAGATATTTTGCACTCCTAAAGCGACTTTATAATGGTTGATACCAACAACACTAGGATCTAATAAACGACTTGTTGATTCTAAAGGATTAATTGCTGGAAAAATACCTAGTGCTGCAATATTACGATCCAACACTGTTTTAGCATCAAGATGGGTAAATGTTGTTGCAGGTGCTGGGTCTGTTAGATCATCTGCTGGAACATAGATAGCTTGAACAGATGTAATAGAACCTGTTTTGGTAGAAGCAATTCTTTCTTGTAACTTACCCATTTCAATAGCTAAAGTTGGTTGATAGCCAACAGCAGATGGCATTCTACCAAGTAATGCTGAAACCTCACTACCTGCTTGAGTAAAACGAAAAATATTGTCAATGAATAACAGCACATTCTGATTATCATGATCACGAAAATATTCTGCCATTGTTAAAGCAGTTAAAGCAACTCTCATTCTAGCTCCTGGTGGTTCATTCATTTGGCCAAAAACTAAAACTGTTTTATCAATCACCCCACCTTGAATCATTTCATAGTAAAGATCATTACCTTCTCTTGTTCTTTCACCAACTCCAGCAAATACACTTAAACCAGAATGTTCTTTGGCAATGTTATGAATTAATTCTTGCACCAAAACAGTTTTCCCAACACCAGCTCCTCCAAATAAACCAATTTTACCCCCCCTAACGTAAGGAGTTAAAAGATCAATTACTTTTATTCCTGTTTCAAAAATATCAACAGTATTTGGTTGTTCATCAAAAGTAGGTGGGTTACGATGGATTGATAGCTTTGGTTTTTTTGGTAATGGTTCTTTTCCATCAATGGGTTCACCTAAAACATTGAACATCCTTCCCAATACATTTTTGCCAACAGGTACCTCTATTGGATGGTTATAGTTAGTAACTTTAACATTACGTGCTAATCCTTCTGTAGGACCTAATGCAATGCAACGAACAATGTCATCACCTATTAATTGGGTTGCTTCTAAAAAAAGCTCACTTTTTTCTAGTTGTACACTCAAACAATCATAAACTCTAGGTAATTGTTTACTTTCTGAAAAGATAACATCAACTACAGAACCAATGACTTGGTGAACTTTACCATATGTTAGGTTTTCTTTTTTTATCAATTTAATTCATACCTCCTATAACTTCAATAATCTCTTCTGTAATAGAGTTTTGTCTTAGCTTGTTAAATTGTAAAGTGTATTTATCAAGTAAATCTTTAGCATTCTTTGTAGCAGCTTCCATTGCATTTTGTCTAGAAGCTGATTCACATAACTTAGTTTCAACAAGGCCACCATACAGAGCCACATCAAAAAACTGTGGAGTAATTAAATTAATTATCGTGGCTTGATCTGGCTCAAAATCAAGTTGTTGATCAGTTACAACCGGATTTAAAGTTTTAAAAGTTTCAACATCGAAAGGAAAAACTTGAAAGAGCTGAGATTGTTGGATTAATGAGTTTTTAAATTTAGTGTAAACCATACAAATTCGATCAAGTTTAAACTCTTTAAATGCATCCATAATCTGATCAAATATCGTTTGACAATAATCAAAATTAATATCACGATCTTGAATATCAATAAATCCAACAGCAGGATTATATTGACTATTTTTATTTCAAAATGATTGGTTTTTTCTACCTAAAAAAAGATTTTATCATCAGCTTTAAAATTAGCTTTTAATAACTTATTCATGTTCGAATTATGCTGTCCACAAAGTCCTAAAGAAGAACTCATCATTATCCATAAAGTTTTTTGGTTATCTGGTTTCTTTTTGGTTCTTTTAAAGAAACAACTACTTGGCCAACAGCTTTATAAAATTCATTAAAAAACAAACTAATTTCTTGAAACTGTTTTTTGAACTTAATAAACTTAGCGCGTGACACCATTTTCATTGCATTAGTTATCTTAATGGTGGATTTTACTGTATTCATTCTGCGCTTAATTTCTTGTATAAAAGCCATTAATTAAGTTCCTCAATACTACCAAATTTGGTAATATCATAATCTGTTAATGTACTGATAAAACGTTTCACAAGCATTTTAAAAGCGGTTTTAGTTTTACTTTCTAAATCCTCAGTAAATTCTTTTTTGTTAGATAACTCTTTAAACAGAGGATGGGAACCATTAAATTCTTCTGTTATCCTTTGTTTAAATTTAGCAATTTCATCAACTGGAATAAACTTAATGAAAGCTTTGTTAATAGCAAATAAAAATAATGCTTCATGGACTTGAGAGTAAGGTTTACCATTTGGTTGTTTAATCATTTCCATTACTCTTTTACCATGCTCTAAAACCTTTTTTGTATTTTCATCAAGATCACTACCAAATTGACTAAAACTATCAAGTTCACTATACTGAGCAAGTTCTAGTTTTAAACTGCCAGTTTGCTGTTTAATCGCTTTTGTTTGTGCTGCACTACCAACCCTTGAAACTGATAAACCAATTTGAATTGCAGGGCGTTGTCCAGCGTTAAATAGACTACTAACCATAAACAGTTGGCCATCAGTAATTGAAATAACATTTGTAGGAATATATGCAGAGATATCACCAGCTTGAGTTTCTATAATTGGTAAAGCTGTAATTGAGCCACCACCATTTTCATCATTTAACTTGCATGCACGTTCCAAAAGTCTTGAATGTAAATAAAAAACATCTCCTGGAAAAGCTTCTCTACCAGGTGGTCTTTTTAACAAGAGTGAAAGAGTTCTGTAAGCAACAGCATGCTTAGAAAGGTCATCAAATACAATCAAAACATCCTTTCCTTTTTTAAGTCAATATTCAGCAATAGTTATTCCTGTAAAAGGACTTAAATAAACCATGGAATCAGAGTCACTAGCTGTAGCACAAACCACTGTAGTGTATTTCATTGAATCATTAACTTCAAGTTGGTGTACAATTTGTGCTACTGATGAATTTTTTTGACCAATTGCTACATAAACACAATAAACATCTTTATCTTTTTGATTAATGATAGTGTCAATCGCAATAGCAGTTTTACCTGTTTGTCTATCACCAACAATTAATTCACGTTGTCCTTTACCTATAGGAAATAAAGCATCAATTGTTAAGATTCCAGTTTCAAGTGGTTGGTTAACACTTTTCCTTTTCATTACACCTGGAGCAATTTTTTCTATTTGATCATATTCAGTAGCTTTAATATCACCTCTACCATCAATTGCTTCACCAAGTGCATTGACGATTCTACCAAGCATAACATCACCAACAGGAGTTTTCATTACACTGTGGGTTCTTTTAGCGGTACTGCCTTCTCGTAACGAAGAATAGTCACCAAAAAGTGCTATTCCGACTGTATTTTGTTCAAGGTTTAATACTATTCCTTGAATATTATTTTCAAATTGAATTAACTCATTTAATAAAGCATTTTCAAGTCCACTAACCTTGGCAATTCCATCAGCAACACTAATGACTTGACCAATTTCACTGTTAAATATTTTTTTGGAATACTTTTTAATTTCAGTTTTGATTAATGCTACGTATTCATTTAGTTTATCTGCCATAACTTCTCAATTAACTTAAATAAAATGGGCCATTATGCGTTTTAATTCATCACGCACATTTTTTTCAAATAAATGGGAACTTGATTCTATCCTAATTCCTGAAATTAAACTGCGATCAATCTCAGTTTTATAAACAACCTTTGTTTTAAAACGTTTTTCCATTATTGCAATAATTTTGTTAAGTTGTTTTGAGCTTAATTCAAAAGCAGTAATTATTTTGATAAATTGAATGTGTTTTTGACTTTCAACATTATCAAAAAAATACTTAATTGCCTGTTCAACTAAAGTAAAGTAACCCTTTAAAATAATTACTTTTAAAAAATCAACAAAAACTTGACAAAAATGATTTTTAAACAACTTATCAACAAGTCTTATTTTATCTGGTTTTGTTAGTGTATAAGAATTAAGTAAGAACGATAATGAACCATCTTTAAAATTACGCATTAATTTCAGAAAAAAATGGCACTCTTCATAAATTTTCTTTACTTGTTTTTGCTCTTCACTTAATTGAAAAAGTGCAGTTCCAAATGCTTGTGCATTAATCATCTTCTGTTTCGTTAGCTTCTAAATCTCTAATAAACTTATCAATAAATTCTCTATCTGATTTTTGATCTATTTTCTTGAGAATTAGTTCTTGTGCAGCCAACATAGCCAATTCCACACTCTCTTTAATAGATTGTTCTTTAAGAGAACGTCTTTCTTTTTCAATTTCCTGACGAGCTTGAAAAATCATTAAGTTAGCTTGGCGATTTGCTGTTTTTTCTATTTCACTTTTTAATTGCAAAGCTTCATAGTTAGCTTGATCAACAATTTCTTTAGAAACTATTAGTGCTTTTTCATGCCTTTGATTAGATTCTTCAAGTAAATTTCTTGCTTGTTTTTCTAATTCATTAGCTTGTTTGATTTGTGCTTCTAGTAAATTTTTACGGTTATTTAAAAACCTTTGAGTTGGTTTTCAAAACAAGAAAATCATCAGTGTTAGTAAGATGAAAAATGCTAGTAAATGAGCAATAAATACCCAAAAGTTAGGGAAAAGTTTATTTATTACTGAACTACTCTTAATTTCTCTAACATTCTCAGTACAAGAAACCAAAAATAAGCTGAGTGTAAAAAAGCTAAAAACTAATAAGCTTCATTTAAAGACAAGTTTTTTTGCCTTTACCATCCTTAAGCTCCTGCTACAAAAATTAAGATAAAGGAAATTAAAAGTCCATAAATTGCTGTAGATTCAGAAACAGCAGAACCAATGAAAATTAGTTTAAAAACCTGTTTTTCAACTTCAGGATTTCTTGCTATTGCCTCAACAGCTTTACCAAAAATATAACCTTGTCCAATCCCTACAGTTGAACCTGCAATCATAGTAACACCAGCACCTATATAAGCACCTAGCTTAGCACTAGCGTTAACATCCTGGGTAGTTTGAGTTTGTTGTAATATAACACCAACTGTAGCTAAAATTTCATTAACATGTTCCATAACTAAATATTTTCTTGAATTACCTTTATGCCTTTAAAGTGTAATTCACTTGCATTATTATTTTCCAAACCTTGATTGCGCATTTTAGCCCAATAATTATAAGTCAACATTACAAAAACATAACCCTGCAATACACCTGCAATTACATCAAAATAGATGTGTAATACTGGGGTTATTAAACCTGCAAAAACTGTTCCTAAGCTAAGTGCTAATGGTTGGTTATTAATACTTGAAAAAATAAAAATTCAAAAGTTATAAAAAAGCGCCAAAATAACTGTGCCCGCTAATATGTTTCCCCATAACCTTAATGAAATAGAAAAAAGCGGTGCAAATCCACTCAATATACTAAAAGGATTTGGAATGAAAGTAGAATACTTTTTTCCTTTAACAGTAATTCCAAAGGCAAACTCTTTAAAAAAATTTCATCTTTGGTATCTAATCCCCATAACAACAATCCCAATAAAAGTTGCAAGTCCTAAAGAAAAAGTAAATGTTAAAGATGATGTTGGTGGTGAAATACCACCAAGCAAGCTAACTAAGTTGCTTGATACTATGTACAGAAGCAACATTAAAAAGTAGGGAGCAAATTTCTTATTTTCCTCTCCTAAAAGATCTGCTGTTGTATCTTGTACCCAAACAAACAACATTTGAAATAAAAGCAAAAAATATGAATTATTTTTTAAAGAATCTGCTTTTTTTAACTTAAGCTTATAAAAAATAAAAAAGTTAGAAGCAGTACAAAAACAATAAAAATACCAAGAATTTGATCAGTAGGGGCAAATGGTTTTCAACCGCTAATTGGTGAGATATCAAAAATACTTTGATTGGAAATGAAATCTATTTGATTAGTTTCTTTTAAAACTATCTCCCGTGGCGACATGTTTTCTTTTCGATTTAGATATTAAATTGATAAAAAAATATTCTAAAGGTAACCAAACAAATGATAACACTAAATTTAGTATTGGAAAAAACAATGTTGAAATTATTGTTGCAATAGTGTTAAAAATTGACGGAGTGACAACAAAAGAAATAATCACAGGAATTAAATAAATTAAATATCTAGCTGTGAAAAAAAATGCAAAGAATGAAACTGCTTTTTGCTTTTTGACATTTTTCAAACTTGCAAAGAAAGATGCTAATCTTAAAACTGATAGTGAATTTAAAACAGCAAAAGGTAATGGCAACATTCAACCAAATAAAATGTCCAAATTATTGCCTTTTGTTAAAACAACAACTGCTATTAAAATTAGTACAAATAATCAAGCTACAAAAATAATTAAGGTAATAATTTTTGCAACCTTTCTATTGTTTCAATTGAACAAAACTTTTAGATGTTAAAGTTTTTAGGTTTAATGTTTGTAAAGGTATTTCAACCTAAGTATTTTCCTTTATCACCAAGTTCAATTTCTATGTACAACAAACGATTGTATTTAGCTATTCTTTCTGAGCGTGACATTGAACCGGTTTTAATTTGACCAGTTTGGGCAGCAACTGCCAAATCAGCAATAGTTGTATCTTCTGTTTCACCACTGCGATGTGAAATTACTTGACTCCAGTTAGCTTTTTTTGCAACTTCAATTGTTTGAATCGTTTCACTAATAGAACCAATTTGATTTAATTTAATCAATATCGAGTTTGTTGTATTTTGTGCAACACCTTTTTTAGCAAGTTCTGCATTAGTACAGTAAGTGTCATCACCAACAATTTGAATATGGCTACCTATGGTTTTAGTTAATTGGTTCATCCCTTCCCAATCATTTTCACTCAAACCATCTTCTATTGAAATAATTGGATATTTTTTTGTTAATTTTTCTAAGTAAGCAATCATTTCTTTGCTTGTTAAACTCCAATCCTTTGTATTAAGGATATTAGCTTTTATTCCTTTCTTGAAAACATAAAGTTTTTTATCTTCGTCATAAAACTCACTAGCAGCAACATCAATGGCAATAGCAATATCATCTCAAGGCTTATATCCAGCTAATTTAATGGCTTCAACCATGATGTCAAGTGCATCTTCTGCAAGTTTTAAGTTAGGCGCAAATCCACCTTCATCTCCTTTATTTGTGTTTAATCCACGCTTTTTTAAAAGATTTTGTAAAGCATGAAAAGTTTCACTAGCCATTTTTAAAGCTTCATGCATCTTTTTAGCACCTAAAGGCATGATCATGAACTCTTGAAAATCAATATAGTTATCAGCATGAGCACCACCATTAATTACATTTAACATTGGCACAGGTAAAACAAAATTTGTTGTATTTAATCCAATTAATTTATTTGAAATGTATTGAAATAATGAGCTGTTTTGTGCTTTTGCTGCTGCTTTTGATACTGCAAGTGAAACAGATAATATAGCATTTGCTCCTAATTTTGCTTTGTTGGGAGTATTGTCTAGTTTAATCATTGCTTGATCCACTGTTAATTGATCAAATGCATTTAAGCCAATAAGCTTAGGGGCAATAACTTTATTAACGTTATCAACGGCTTTGTTAACGCCTTTACCAAAATAATTTTTTGGATCATTATCACGTAATTCAATTGCTTCTTTCTCACCTGTAGAAGCACCTGATGGAACCATCGCTTCACCTACATGACCAGATGCCAATTTAACAACACAAGCTACTGTTGGAACACCCCGAGAATCAAAAACTTGATAAGCAAAAATATCGGTTATTTTTGAATTGATGTTTAGATTTGAACTTCCCATATTAAATAGAAAAATATTGTTAATAAAATTATTATATGTTTTAAGATTTCTATTAATATCAAGTAATATGAAAGAAATTTATTTTGGTGGTGGTTGTTTTTGAGGAATAGAAAAATATTTTCAACTTATTAAGGGTGTTAAAAAAACATCTGTTGGTTATCTCAACTCTAGGATTAAAAATCCTAGTTATGAGCAGGTTTGTTCTGGTTATACTAATGCTGTTGAAGCTGTAAAAGTTGAATACGAAGAAAAAGAAATTTCTCTTTCAGAATTAATTGAAGCACTTTTTGAAGTTATTGATCCAACTATAAGAAATAGACAAGGTAATGATATTGGAACACAATATCGTACTGGTATTTATTGAACTGATAGCAGTGATGAAAAAATAATTAATGATAAGTTCTTAAAACTTCAAAAAAACTACAGTAAACCAATTGTTACAGAAAATAAAAAAGTAGAAAATTATTATCTTGCTGAAGAATACCATCAGGATTATTTAAAAAAGAATCCAAACGGTTATTGCCACATCAAATTTGACTAATTAAATATTTCAGGATTTTTAATAAATAAAAAGGATTTAATGATGTTATAGCAATAATCATTAATCCGTTCAATATGTTTAACAGAAGCTACAAGATCAATTAAGAAAATCCTTTCAGTAACCTCATTTTTATTAATGGTTTCATAGTATTTTGTTAATACAAGGCGATATTGGTTCTCAAATTCAATAATAGTTTTGGTTGCATTTTCAAATTGTAATGTAAGTGGCTTTTCTTTATCTTGTAAACTTTCAAAGGTTTTCTTAAGGTTATTGAGCGCACTTTGATGCAAAGTAACTAGATTATTAAAAATCTTTATATCAAGATGCAAATAATGTTCTATAAACTTAGTTAAATTATTAGCATAATCACAAATGCGTTCCAAGTCACGACTTGACATAATTATTGTGATTGTTAGGCGTAAATGGCTAGCTAATGGAGAATTTTTAGAGATTGTCCAAATTGATTCATTAATTAATTTAAATTCAGACCTATTAGATTGGTCCTCCATTTCATAAATAGTTTTAATCAGTTCTTTGCGTTTTTCTAAATTATCTTCACAAAGTAATTGATTTAATGTTTCATGTGCATTGATTATGTGCTTAAAATACTCAAAAAAAAGCTTTAATAATTTCTTTTCTGAACGCTTTAAAATTTGGTAATTAATGTTTTCCATAGCTAATTTTTCCCACTTATATAACTATTTGTTGCTTTTTGCTTAGGCTTTGTAAATATCTGTTTTGTAGTGCCTTGTTCAATTACTCTTCCATCAGCAAAAAAAATCGTTTCATCAGTTATTCTAATTGTTTGAGCCATAGAGTGAGTAACAATAACAATTGTGAATTTTTCTTTTAGTTGTTGAATTAGAAGTTCAATAGAGTTTGTGGCAATTGAGTCTAAAGCACTGGTAGGTTCATCCATCAAAAGAACATCTGGTTGTAAAGCAATAGCACGCGCAATACACAAACGTTGTTGTTGTCCACCAGAAAGGGTGTTTGCATTCCTATGTAAATTATCTTTCACTTCATCTCACAATGCTGCTGATATCAATGCCTGTCTTACTATTTCATTGATAGCATTTTTATTGTGAATACCATGTGCTCTTATGCCATAAGCAATGTTTTCAAAAATAGAAAAATTAAAAGGAGTTAATTTTTGAAAAACCATGCCAACACTAGTGCGCAATGTTAAATCATTAATAATTCCTGAATTGATATTCTTACCAAGAAAATATATGTCACCTGTTCAACGTGTGTTTTCATTTAAATCATTTAATTTATTTAAGCACCTAATAAAGGTGGATTTACCGCATCCTGATTTACCTATTAAAGCAGTTATTTTATTGCGTTTAATGTCAAGATTGATATCAAATAATACTTGTTTGTTTTTGTTATATCAGAAGTTGAAATTTCGAATTTCAAATACATTTTCTTCATCAAAATCCTTTTTAATGACATTATCTTTGGTGTTTTTAATCTTTATTAGACGGTTTGAAATTTCAATTTCATTCTTAATACGGGAGATTTCATTTGCATCTGTTTCATTATTTAAACCAGTAATTTTCTTTTTATATTCTTTGATTTGTTGTTCATAAAGCTTTCGGTAATGCTTAATTTTTTCAAGCTTCAATTGAAAATTTTTTCAAAGTGCTTTTATGTTCTTTTCCATAATTTAAAGGACTGAAATTTACTTTTAATTACTAGCCATTTTTGTTTATTTAACAAAAATAAACTCGGGATTAAATAAGAACTGAAAAAGATTAAAAGAATTAAGAAAACAACAGAGACCAATGATGTTTCTAACATAACACTTATTGCATTGCTATTAATAGAAAATAACTGTCCATATATCCTTGTTGTTAGTGTTTGACCTGGCAATGACAAATGAAATAAATTACTAGATGATAACCCTGAAGTGATAAAGAAGGGTGCAGTTTCAGCAATAATTCTGTTGATTGACAAAATTAATGCAACTATTAATCCTTTCAAAGCACTAGGTAAAACTATTTTGAAAATAACTTCACGTTTACTTATACCTAAAGCAAAAGCACTAATTCTTAAATCCCAACTGACATTATTTAGTGCTTGTTGACAAGTTCTTATAAGGAAGGGTAATATAACAACACTAATAGTTAGAATGCCTGCTATTAAACTAGTACCATTAGCTCCTCCAGCACTTAACTGCAGGACTCTTAAAAAGAAAGAAAGTCCAAATAATCCATAAATAATAGATGGCATTGAACTTAGTGAATCAATTACAAAGTTAAAAACATTTTTAACCACTTTTGAATTATTGTACTCGTTAAGTCAAATTGCAATTAAAAGTGCTAGTGGAAAAGTAATGGTGATAGTAATCAAAATAATTACTAGAGTATTTACTAGTGCTCTGCCAGTTGAATCAGCTTCAAATGAAAAAACAGTAGAACCATTATTATTAATAGCAACACTTCCATTAATAAATACAAACAGCAAAATTGCTAACACAAATCCAAAAGCTAAAAAAGTACAAATTAATTCCTGGAATATTTTTAAAAAATAATTAAGTTTTATTCAAACAACACTTTGAAGCCTTTCTTTAAAAAAAAGTGAACGTTCATTGATGTTGTTTACTTTTATTTTTTTAACACTTTGTCTTGTTGATGTTAAATCAACAAAAAGTGCACTAATATTATTGGGAATGAATCACACAACTTGATAAATAAAATTACTAATCTTTTTTAAAAAGGGATAGCGTTCCAGTGTTTTTGGATTAGCTGATCAAATGGCAAAGAAATTTAACAATGAAACTAATATCAAAATTATGATTCCAAAGATATATAAAACACCATTAATCTGTTCATCTCCATTTTCTGAAAAAATAAAAGTGGAAATAACTGATCCCAGTGTTTTTAAATCAGAAGTAAAAAAACGATTGTTGTTAATTACCTCTTGATAGTTAACACTCTGCAAAACAAAGTTAACAGCCATTGTTTCACTAATTGCTCTCGCAAAGGCTAAGGTCAAAATAACTGTTAATTGTGGTTTAATTTCTTTTTTAATAATTTTGTAGATCGCACTTGTTTTATTTTCCCCTAAGGAAACAACAACACTAATTAGATCGTTATTTACATAAGTTAATGTATTTGTTGTTAATGAAATAACAATAGGAATGATCATAAAAGAAAGCATAGCTATCACATTTAAAAGTGAAAGCGGCGGTAATTTCAAGATATCCCGAAAGAAAATGCTTAATATTTGTGATGCAAATAATCCAAAAATTACAGATGGTATTCCTGAAAGGATATCAATAATAAGTGAAAGTTTTTTTCTTATTTTTGGTTTGCATCGATAAACAAGGAAAAATGAAGTTCTAACCCCTATATAACTAGCAATAATTAAAGCTCCTATTGATACAATAAAACTTACCAATAAGGGGAATCAAATTCCTGCCTGTTTATTACCTAAATTAAATTCAAGATTAAACAAGGACTTTGCAAAATCAGGTCCAATTTTTGTTGCTTCTGTTAAAAGAAAAACAAAAAAACTTATAAATAAAACTAAAAACAAGAATGCTAAAGTCTTTGAAAAGATTCTTAAAAGATTCTCTTTTTTTAAACGTTTTTTAATCTTCTTTTGCACTAAAAAGCTCCAAAGATAGGATTACTAAAAGCAAAGTCATCAACTCAAAAATTTTCTAAATTCATTGGTGATGAGTCATTTGTTTTAAACATCTTCTTTTTCTCATCTTCAGTTAATTGCAAAATACCAAATTCATCATAAATATTTTTAATTACATCTTTTTTTCCATTAAAAAATAATCAATTAAAAAAAGTTTTAATTTCATCAATATTCTTATTCTCTTCAGAAAGTGAAACTATTGAATTTAAAGGACGTACTCATTTATACTCATTTTGGGAAACAGTTTGATTAGAAGGCAAAACTTCCTTATTCTCATATTTAATTGGTAGTATCTCAAAACCATTATTTTTAATAGCTTGAAGGTTGTTTTGAATAAATCCTAAACTAAGGTAAATCATCCCGAAAAGATTAGGATCTTGTAAGTTAGTTACAAAAGTATTAAAAGCTTCAATATTTGTTTCACTAGTAGGTTTAGCAAGAGGTCCATAATTAATAATTCCTTTCAAAATATCTCTAGTTTGAGAATCTAAAGTTTTATCTGATTTTAAGGCGGAAAAATTTAAAAAAGCTTCTGCTGTTCCTGAAGCAAATGACCCACCAGTTCTTGGAAAACTTGTTAAAGGGTAATCTTTTTCATTTGACATATTTTTTGTATCCTTTTTTACAAACTTATCAATGTTAATTGTTTTAACACCAGCAAATAAGTCGTAGAGATCATTTATATTATCTTTTGTTAAAAGTAACTTACCTTTTAACTCCTGTGGTGCCTTATAAATTACTGCAATAGCATCTTTGCCAATAGTTAAAGTTTTCAGCTTTTTATCTCTTCATTTTTTTTCATTCTCTTTTGCATATTCCTTAGGGTTTTTAGAGACATTTCCGATATCAGCAAAGCCGTTAATGATTGCTCTGATTCCAGCATTTGATCCGCCTGCTTGCACACTAATTTCAACAAGCTTATCATTGTCATTATTTTCATTTAGAACATAATAAGAACTTAATTTATTTAGTAAAGGTTGAACTGAAGAAGAACCAACAGCACTAATCAGGTTTATGTTAGCACAACCACTAAGAAAAAAAGCAGATGCTAATGTTAGTAGCGTCAATTTAAAGAAATTTCTAAACTTTAGCACTTTTTAAACAGATTAAATCTTTTCTAAAGAGAATTCGAATTGACACTCAGGTTATTATTTAGTCTAGTTTTTTCACAAGCCAATTAATTTAAATGAAATGCATTTTTATTAATTGCAAATTTAATTTCTTTTCTTAACAATTTTCTTTTTTCTTCATTTTAAAAACAAAACAGCAGAACTTATCATTAAGCTAATGATAATTCCAAAAATGACTCATAATATTAACTCAACATTTGTATCTTGTTTTTAATTTCATTTTTTTCTAACTTATCAACTTCATAGGATTTATCTTGAGATTTAAGATTAAAATCTGGAACTCTCTTGAATTGCAGGTTATTTTGTTTTAGATAAAATGATAATGCTTTGAAAAGTTGTGTTTGATTTTCACTAGTAATATCAAATCAAATAGAATTATCAAAACCATTGGCATCTACAATTAAAAGTGTTTGATTATTTGTTATTTTTAAATCATAGCTAATACTAAATACTTGGTTATCATTATTAACACCTTTAATAAATAATTTTTTTGATCTAACATCGACATCAAAAACTAAATTAAATTGATTTTTATTTTCTACTAAATGGATGTTAATAAATTTATTCTTTAGGTTAAAGCGGACGTTTTTAGTTGTTTGCAATCTTTTTTCACTTAGAGTAATATTGAGTTTATCAACTAGTAATTGTTCGTCATTTAAACTAAAAGGTTCATGAAAATCAAGTTGATAATCCTCTAGAAATTTAAATGAAGATGACTTTAAATCTAATTCAGCAATTTTTAAGTTATCAACTGATAAAAATACCTTGTCTTTTTCTAACTTAAATCCCAGGTCATTTTTATCAACAAAAAGTTTGTTAGTATGAAATTTAAATAAATATGTTCTTAATTTAGGGTTTTTTTGATTTTGTGATAGAAAATACATTTTGTCTTTAATAATTTCAGAATCACTTTCAGAACCATATTTGAATAGTTCATTGCTATTATTTATTTCTGCTAAAACATAAAATATACCTTTCTTTTCATTAGTAGGCTCTAGATGTTTGAAATATTTTTTAGGGTTATCCACAAATGTTAAAGAGTTACTGTTGATATCTAAATCAATATTCTTATCCTTAGATTTAAAACTATTTTTGATAATGAAAGCCATTTGTCTTTTTTCATAATCATTAAACTTTTCATTACGTTTTTTAAGTTCAGCAACATTAAAGTTTTGAACATTTTTATTTAGTGGAATACGAAAATCTTTAGCAATTCAACGCTGTTCATTAAAATCTTTTCTATATTTAAATTTGTATTCAAAACCATCCTTAGTTCTTTTGATAGTTGAAAGTTTTTTAATACTGTCAAACAGTGGTTCAACACTATAGTTACTAATATTTTCTGATTCATATCCATCTACAAAAAAATTAAGTGCTAAAAATTCAATATTCATTTCATTATCTGCTTTCTGTTTTTCTGTTAAAAACTCCTCAATTGAACTTAAATCAAAATAATTCTTGTTATTTCTTAACAATGTTATTTCATTTACAGAATTACCAATTTTTACTTTAACAGTTAATGTTGATCTATCAAAAATGGTTTTGACCATTCTTGGTAAATTTCTAATAAAGAATAGTTCTTCTTTATTGTTTGGTGACATTTGCAATAGGTTGTATAAATAATCTAAATTTGGATAAAAATTACTTTGATTATTGGTTGAAATATTAAAGCTTTTTAACGCTTCATTTTTATCAATTGATTCATCAATTCTTTGATTAATTAAACTAACTTCTTTAATTGCATTTATGAATTTATCTTTAAATTTAATAGTTGGTTTAATTTTTGTACCTATTGTTTCTTTATTTAGAGTTTTTTCAGTTTGACTAACAAGCGAATTAATATCAAATTCCAACTGTGAAATTCAAAGCTTTGAAAGATTACTGAAACTAATTGGTAAAACTTCATCAGGATTATTTTGCCTTAGATAAAAACTTATTTGATGATGAACAACTTCTTGTTCAGGTAATATAGGTGTATGTTTAAAAATATCCCATTTACTGTTGAATGCTTCACGTCTAATATCTTCAGTTTCTGGTCTAACTACTAATCCATTACCTAGTCTTAATATCGTTTGAGTTATTTCACGATTATTAGTAAATGCTTCAATGATTTTTCTATCCTTTTTCATTTTCTCTTTAAACTCATTTATACTCATCCAATGAGCATCAACTTGTTTGTTGCTGTTAATAGAAAAAAATCAACTGCTTCTACCAGGAAAAGGTTCGTTTCACTGAGCGTTATTCTGTTCAAAAACTGGAGGAACTTTCACTAGTTCTAAATCTCATCAGAAACGATAAGCTCTAATTGGTGTCATTGCTAAACCTCATCAGAAATCTGGAGCTACAAAAATATTATTTTTTCAGTCAGCAAAAAATCCTCTTGATTCTTGGCCAAGATAATATCACATAGGTGCATTAAAATCAATTACATAATTACCATTATTACCTTTTCTTAATCATTTAATATTTCTAATAACATCTTCTGGGGTTGGTTTAATCATTCTTAAAGTCGATCCTCACATGTGAACAAACTTAGCTTCAATAAAATATGCTTTTTTATGACTGGGCTTTGTAAGGTCTCAAAAATAAGGATTATCAGGTTTATATCAACTTTTGTTTCAAGAACCATCTGGATTTGTACCAGTTATTTCTATATTCCTAGAAGGAACTTCGGTTCATTTTTCATTAGTATGACCATCTCTTGGAAAAAAATTAGAAATTGCTTGACTATGAAAATAAAATCTCTGCTCACTATGGATATCAGATGACCATGAGTTTCTAATTCCAGGGTATCTAAATGTAGATGAAAATGCATGTGCAATATAACTACTCTCAATTTTTAACTTTATGTTCTTTAGATAATAAATATTGTTGTATCAGTGAAAGTTATTATCTCTAATAATTTGTTTGTAATTATCACTATATTTATTAACTAATTCACTTCATTCATTCGAATAAGTTTTTGCAAAATAATTATTTTGATTAGGTCTTTGAATTACTTCATTATCAAAATTAAAGCTTTTTCGAAAATAATCGTTTTTTAAATTAAATCCAGCTGTATAGCTTCTGTTTTGAATTGTGTTTGGAGAGAGATAAAGATTAGTATCTACCAAGTTTTTTTCAAGTGAAGCATTAGCTAAAGCAACGGGAAAAAGAAAAGAAACTAATAAAGGAATAAAAAGATATGAACGCATAATTTATCTCATTAAATAAATGTGTATTTAATGAAATATTGACAATTTTTATTGCTAGTTCTTGTTAAAAATTATCTCTTGTTTTATTTTTTATCATCTTTTGTTTTTTATTTCACTTATGAAAGTAAATAAAAACAGTGATAAACAAAACTAAAACACTAAAACCAATAATCAAATATACTATAATATCTGTTTTTTTATCTTCACTATTAGATTGAATAATTTTGTCTATTTGAAAACTATAGCTGTTTTTCTTTAAGCTAAAATTAGGTTCTTTTTTAAATTGAAATTTTTTTAATGAGAGAAATAACTTAAGCTCATTTTTCAGTTTCTGATTATTTTTCTTATCAAGATCAAACCAAATATATTTATTTAAGTAGTTATTTGTTGTTCTAATTTGCAATTCATTTTCATTTTTGATAGCAATATCATAAACAAGGCTAGTTAATCTATTTTTACTTATATTAACTTCAATATTGAGCTGATTTTTAATTGCATCAACATTTAAATAAAAATGAAGCGAACTATTTATCTTTAGAGTAGTTAATTTGATCTCTTTAGGAAAAAGATCTAAGCTAGATTTCTTTTTATCTATTAAAAGATTCATTTCACTTAAAGCCAAATTTAGTTTTTTAATTAATAGTTCTTTATTAGCTAAAGAAAAATAATCATTTATATGAACCTCATAACTTTCAAAAAATGAGGTATTATTTTCTTTTGGATTAAATTCATCTATTTCAACATTATTAACAATTACCCTTAATTTATCACCGCTTGATTTAAATCAAATATCATTAACATTTAAAACTAACTTTTTGGTTCTCATTTGAAAAAGATAAGTTCTTATTGCAGGAAAGTTTTTATTTTGTTCTAAGAAATACATCTTATCTTTAATGATTTCGCTATCATTTTTAGAACCCCATTTAGTTAAATCATTACTGTTAATTATTTGTGCTGAAACATAGAATATACCATTTTTAACACTGGGCTTAATTAAAGATTTTTTAATTAATTTCTCAGGATCTTTTATGTATTTTATTGTAGCGGATGAAATATTTAAATTGTTTTCTTTTTGAGAAGTAAAACTATTTTTAATTGCATATGTAATTTGTTCTCTTTGATAACTATTTAATCCGTTTTTTTCAATGTCATTAACACTGAAATTTTTCTGATTTGTAACAATAGGAATTAAAAAATGATCTTTTAAAATATGATGTTCATTGTATTCAGATCTAAACTTTAAACTATATTGAAAACCATTTTTAGTTTTCTGAATAGAAGGCAAATTTTTAACTGACTTAAAAAAAGAATCAACTGGTTGATTTTTTAATGTTCATCCTGTTTTATTCACAACAATAAAATCAAAAGTTAAAAACTCAATTTCATAAAAATTAATACCAGTTTGATCAGCATTTAAGAAATTTTCAACATCACTTAATAAAAAAACGTTACTGCCATTTTCTATAAAAGTAATTACTTTATTTTTGCCACCTATCTTTATATTTATCGTGATGGTAGTATCTTTAAAAATACTTTTAATTAGGTTGGGTAATATCCGAATAAAATTTAATGTTTTCTCTTGTTTTGGATTAATGTTAATTAAATTACTTAAGAAAAATATTGCATCAATTCTTTCATCTTTTGGTTCAAAATCAAATTTAGATTCTTCCTGTTTTTCTAATAAATCTAAAGCAAATTGAAAAAAAGTTTTATCTTTATTTGCTAATTGATCAAACTCTTGATTTAGCTTACTTACCATTTCAATTGCATTAGAAAATTGATCACTAAATTTCAGCTTTGGTGTTATTTTTGATCCTATTAGTTCTTTGTTTAAAACAGTAGTTGGATTAGCTATTAAAGAATTTGAATCAAATTCAATTTGGTTAACAATGATCTTAGCAAGTTGATTTGTTGCTATAGTTAATTGATTTTGTGATTGAAAATCATCTTTAACTAGATAAAGATTAGTATCAATCAACTTACTTTTTATGGAAGTGTTAGCAAATAATAAGGGAATTATCGCAAAGGAAACTCACTTTAAATAGCGTTTTCATGACATAATAAGCTTTATATTCCATAAATTAAATAAAACTTATTAAGTGACAGTTTTTTTACTAACGCTTAGTAAATTGTGGTGCACGTCTAGCACCATATAAACCAAATTTTTTGCGTTCCTTAGCACGCTTATCACGTGTTGTTAATTTTTGGTTTTTAATAACTTTTTAAGATCTGGATTAAATTTTATTAAAGCACGAACAATACCTAATCTAATGGCTCCTGCCTGCCCAGTAAATCCTCCACCTTTAACAACAACATTGATATCAAAGTTATCCTTAAGTTTGGTTAACTCTAAGGGTTGTTCCATATCTTGAATCACCAATTTATTTGGAAAATAATCGCTAGGATTACGATGATTAATGGTTATTTTACCCTTATCTTTGCTTTGATATAAATAAACTTTAGCAGTAGAAGATTTACGACGACCAAGTCCATAAAAAGATTTTTTATCCATATCTTTTAACTTCAATTAAGTAATGTTGGTTTTTGTGCTTCCATGTTGTGCTTATCATTCTTAAAAACATGTACTTTAGTTATTAATCTTCTGCTTAAACGATTATCAGGTAACATTCCCTTAACAGCATTGAAAACAAGTTTATCTGAATTTTTGTTTATCATATCCCTTCCACTAGTTTTTTTAATTCCACCCATGTATTGAGAGTGATGATATCAAAACTCATTGTCTTTTTTATTTCCAGTTAAAACCACTTGATCACTGTTAATAATTATTAGATGATCTCCACAATCTTGATTAGGAGTAAAATTAGCTTTATTTTTTCCTCTAATTAAATTTGCAGCTTTAACTGCTAATTTGCCTAAAACCAAACCACTAGCGTCAACAAGATACCACTTCCTGTTTTTAATGGCTTCTTCCTTTGTAAGCATCGATGTTTTTTGCATATATTTAGATACAAAACCGCATTCTATTTTAATATTATTACTTTTCTTCAAAAAGTCCTTTAAAGTATTTATCCACCATTTTTTGTGATAAAGAGCGTTTCTTTTCTTTTAAAAAAGGCTTAGCTAAGTTTTCCAATTGGGTTAAGTTCAACACTTCAGTTTGATACTTTTGATTTTTTGTTTCTTCAGTAATTGTTGTTTTATATTGATGCTCACTAAAAGATGCTAAGAATAAATTTTGGTTAAAAATCTGAAATATAGTTGCTTGTACATTACCTTCTTCTATTGCCTCTTTTCAAAGTCTCGTAGCAATTTTTTGATCTTCAAATAAGATAACTATCCCAAATGAAGAAGCTTTTACTATTTTGGTTGCTTTCACTATATCACGAGCACTATATTTGTCTATAAAGTTATTGAAATAACTTAAATTATTCTGTTTATCTTTATCATTAAATTTAGTTTGAAAAACACTTAAAAGATTTTGAAAATGATCAATTCATTTATTGTCATTAAATGCATTAATTGCAAGAGCAATATTGGCAAGATTTAGTTTGCTTGTATCTAAGAATTTGTTATTAGTAACTTCATCATTTTTAGGTGTCTTTTCTGATTTATCTATAAAAGATTTTTCAAATAAAGAAAAGTGCTTTTGGTCTTGTTTGTGATTTGATTTTTTTATTTTTTTCTTTTCCAAATATTCAGTTTCTAAGTGTAGAGATTTTTCATTACTTGGTGAGCTTATTGTTAAAGGTTTTTGATTAAAAAATTCATTATAGCTAATAACAATAGCTTTTAGCCTATCAATTAAAGTTAATTGACTTAAACCAAAATTAGTATTTTCTTCAATGGCCTTAATTAAAAACGCATAATGCTGCTTTTCAAAATTAAGTGTTTCTATCATTGTTTTTTCAGTTGAATCTAAAACATTAATATTTTTTAGTTTTGCATACGCATATAAATTAACTGTTAGCGCAAACAACTCTCTTAAGAAGTAAGTGAAATTTAGACCATTACTTTCAAAATCATCAAGCAAGTTAAATGCTTCTTTTATATCTCCTGATAAAACTGCTTTAATAAAAGTAAATTTTGCATTTCTATCAACGATATTAAATGTTTTTTCAACATCAGTAATACTAATTTTTTCACTATCAGAGAAATTGCTAATTTGATCTAGTAAGCTAAGCCCATCACGCAATGAACCTTGGGATAAATCAGCAATTTTTATTAATGCATCTTTCTCTATCTTAATCTTTTCTTTTTTTGCTATATCATTTAATCTTTCAAGGATTAAATCACTAGTTATTTTTTTAAAAAAGAAGCTTTGACATCTGGACAAAATTGTTAATGGAATCTTGTTAAATTCAGTAGTTGTAAAAATAAAAAGAACATAAGGTGGTGATTCTTCTAAAGTTTTTAACAAGCCACCCCATGATTGGGTGGTTAACATGTGTGCTTCATCTAAAATATAAACCTTTTTTTTAAATGTGAAGGGATGATTGAAAACATTTTCTACCAACTCTCTAATATCATTAATACCATTTTTAGAAGCTGCATCTATCTCAACTATATCAATGGCACTATTAGTGTTAATACTTTTACACACATCACAACTATTACAAACATCAATTTGATCTCAATTTAAGCAGTTTATCGCTTTTGCTATTATCTTTGCAAAAGTAGTTTTACCTGTTCCTCTTTCACCTGAAAAGATATAACCATTAGGTAGTTTATCCCTGTTAATAGCATTCACCAAGATTTTTCTTATCGATTCTTGTCCTAGGGTTTGTTTGAAATTGATTGGCCGATATTTTTGATAAAAAACTTGGTGCATAATTTTTAAATTGAATCTGGTTTTAAAATTTTGGACAAATATTGGGCAGTATAAGATTTATTAACTTGGTTTATAAGTTGTTCAGGTGTTCCTTGAGCAACAATTTGACCACCATTGTCACCACCTTCAGGACCTAAATCAATGATATAGTCAGCAACCTTAATAATATCTAAGTTATGTTCTATAACAACTACTGTATCACCATTCTTAATGATTCTTTGAATTATTGTTAATAGTTTGTTTATATCTTCTAAATGTAAGCCAGTAGAGGGTTCATCTAAAACAAACAAAGTTTTACCAGTAGATTTTTTTTGTAAAAACTTAGATAACTTAATTCTCTGTGCTTCCCCACCTGAAAGAAAAGTGACATTAATACCTAATTGCAAATATTCTAAACCAACATCACATAACAACCTTAGTTTACGTGATATATTTGGGATAGCTTTAAAAAATTCATAAGCTTCTTTACAAGACATTTGTAAAACATCAAAAATTGATTTTCCCAAATATTTAATTTCCAGTGTTTGTGAATTGTACTTCTTGCCATTACATACTTCACATTTGACATAAACATCAGGTAAAAAATGCATTTCAATGCGAATCACACCATCACCAAAACACTTATCACACCTACCACCTGGAACATTAAAAGAAAATCGTGAATTTGTATATCCTCTTGCTTTAGCTTCTTTTGTGTTGGCAAATAAATCACGAATATCATCAAAAACACTAATATAGGTTGCAGGATTAGAACGTGGTGTTCTACCAATTGGGTCTTGAGAGACAACAATTATCTTATCAATGTTGTTAGCACCAATTATTTCCTTATATGTATCTTTTTTAACACCTTTACGATAAAGAATTCTTTCTAAAGCTGGAACTAATGTTTGATTAATTAAAGAGGATTTTCCAGAACCTGAAACTCCTGTTATCAAAACCAATTTATTTAAAGGAATCGTAACATTAATATTTTTCAAATTATTAACTTTAGCACCCTTGATAATAATTGTTTTACCATTACCACTATGTCTATTTTTTGGAATGAAGATTTGTTTTTTACCACTAAGATATTGTCCAGTAATTGAGTTTGAGTTTTCCATTACTTGTAAAGGTGTACCGCAAGCAACTAATTCACCACCTTCATTACCTGCTTTAGGACCAATATCAATTAAATAATCTGCCGCTAACATTGTTTCACTATCATGCTCAACTACTAATAAGGTGTTACCTAAATCACGCATTACCATCATTGTTTTAATTAAACGCATATTGTCTTTTTGATGCAATCCAATAGAAGGTTCATCCATTACATATAAAACACCAGTAAGTTGAGAACCAATTTGGGTAGCTAATCTAATTCTTTGTGCTTCTCCACCTGACAGCGTAGAAGCTCTTCTTGCAAGATTAAGATAATCTAAACCAACATTTTTAAGAAAAGAAAGACGATTAATAATCTCTTTTAAAGCTAATTCACCGATCTTCTTTTGCTCATCATTTAACTCTAGTTTTAATAGAAAATCAATACTTTTATCAATGGAAAGTTCAGTAAAGCTAATAATGTCAATTCCTCCTAACTTAACACTTAAAGCGTCTTTTATTAATTTTTTTCCATGACACTTTTTACATGTTATTTCAGACATGTATGCAGAATATCATTCTCTACTTACTTGGCTGTTTGTTTCTAGGTGTCTTCTTTTAATTAAATTAGCTATCCCTTCATAATGCTCAAAGCGGATATTCTTAGCACCTGAATTGGAAATTGTTTTTATTTCAATAGGTTCATCACTTCCTTCTAAAATTAAATTAAGTTGTGACTTATCTAACTGTTCAATTGGTTTATCTAATGGAATTTTATAGTGATTAACTAAAGATAAAAAGCGCTGTCAATCCAAAGAAGTTCCATGCACAATATTTTTAAAAATATCAATGGCTCCTTGGTTAATAGAAAGTTTAGAATCAGCAATTATCTTGTCTACATCAGGTTCATAACTAAAACCAAGTCCTTTGCAATATGAACATGAACCTAAAGGGGAGTTAAAGGAAAATAATCTTGGTTCCAATTCACTAATAGAAAAACCACATTTATCACAACCATGATTTTTGCTGAAATTTAATATTGTTCCATCTTCCTTAAGAACTTCTATCTTGCCATTAGTTAACCTATCAATGGTTTCAATGCTATCAACTATCCTTGAATAAGTTTGATTATCTTTATTGATGATAATTCTATCGATCACTACACTAATGTTGTGTTTAGTATTTTTATCAAGTTTAATTTCATCATCTAAGGTGTAAATCTGGCCATCAACTAAGACTCTAAGAAAACCTAATTGCTTGTATTTAATAAATTCATTTGTAAAAATGCCGCGCTGATTTTTAACAGTAGGTGCTAATAATTGCACCTTTGATTTATTAGGTAAATCAAAAATCTGATTAGCAATTTGGTTAATTGTTTGCGTTTGAATAGAACCATGACCATTAGGACAATAAGGGGTCCCAATTCTAGCTCATAAAAGTCTTAGATAATCATAGATCTCAGTTACTGTACCCACAGTTGAACGTGGGTTATGTGAAGTGGTTTTTTGATCAATGGAAATTGCTGGTGATAATCCTTCTATAAGATCAACATCAGGTTTATCACTGTTACCTAAAAATTGGCGTGCATAAGAAGATAGAGACTCTAAATATCTTCTTCTCCCCTCAGCATAAATTGTGTTAAATGCTAAGGAAGATTTACCTGATCCTGATAGACCAGTAATAACAACAAATTGATTTTTAGGGATATCAATGTTAATGTTTTTAAGGTTATTTTCTCTAGCACCTTTAACCCTTATAAAATCATTATTTTTTCATTCCGGTTTCAAAGTCAATGTTTTTAATTGAAAAATTTTTAACTAACTTATTATTAGGATCATTGAAAATAAGCGCAATACCTTGTTTATTTTGATATGAGATCAAAAGTCCACGAATGAAATTATAAGCGTGGTTAGTGAAATCATTGCAGCAGTTTATCATTAGTAACTTAGGATTTAACAAAAGTTTAAATAGCAAGTAAAGCTTTAGACTTTCATTTTTGTTAATTTTGTTTAAATCACTAAATAACATTCAGCTGCTAATACCATTCTTTTTGGTTTGATTTATCAATAAATTAATGTTGTTAATAAAGTTAAGTTTTTTAAGATTTTTTTGTGATCTTAAAAAGAAATTAATTGTTTTTAATTGTGAATTCAAAACATTAAGATAAAGCTTATTTGAAGCTTCTAAACGATTGATATTGCACTCTTCTGAATCAATAAAAAAAGTTCTCTTAATTTTCTTGTTTGTTTTTTTAAGTCTTAAGTGTAATGTTTTGTTTAAAAAACAATATCTTTTCATGATGAATTTAATCTTTTGTAACTTTTGATTTGTTTCATCGCGAAAAATTCACTTAACTAAATTGATGTCTTTTAAAAGTAATTTGTATTTTTGTTTGCATTTTTTTAGCTTTTGTTTTAGATCATTCTTGGTTTCTTTATTTGTTGTATCTGTTGTTGAAGAATAACCGCGTGATGCTAAAAGGAAACTAACAGGGGTTTCAAAATTTAGATCTAAATATTTATTAATAACTTGGTCAACTAGAAAATTTCACTTTGGGGAGTTTAAATCATTTTTTAACTTATCTTTTAGCAAACTAAAATGTGGTTCACTACGTCCTTCATTTCAAATAAAATCATTAGAGCCACAACTTAAAAACTCATATTTATTAAAGAATTGTTGAAATTTTTTACTTAGGCTATTTCAAAGTGAAATAAGAACAAAATACAGGTTATTTAACTCATATTTTTGTCAGTGAATTTTTTGGTAATTAGCCAATAAGCTTTCATAAAAATATTGCTTTATTTGAAATGCAAAGTTAAAAGTCTCCAAACTCTTTCTCATGCTGATTAAACCAATTGCATATTGCACTGTATCAATAAACTGTTTGAAAACTGTTTTTTGGATTAGAACTGAAAAACTGTTATTATGCTTACCTCTATAATGATTTTGATTTAATAAGATGTTTTCTTTTAGTTCATTAACTAATTTTTCATGAACATATAAATTGTTTGATTGTTTAACAAAATTTTTAATAAAAGATAAACGTGCTTTGATCAAAAATTCATGAACCTCTTTTTTCGTTAGAATGAAACGTGCAGACATTAGTTTAAGTTCTTGATTAATTGTCTGGTACTTTCAGTTCAAATTAGGACCTGATTTTAAGCTGATAATTTCTTGATAAAGATTTTGAATATTAGCAAAGATTTTATTGACTAAATTAATACTTTCATCAATAGTTTCTACAAGTAATTTTGGAGAAAGTAAATCCATCTTCTTAATTTCTTTCAAAAGCAAAATTCTTGTTTTAATCCGCTTAGATACAATCTTTTTTTCTAAAATTCTTTTTCTATAAAAATTAAAAAAACTTCTTCACTTAATCTTTCAACCACTAAAAATTAAAGAAAAGGTAAATGAATTAATTAATTGCAAAGCATTTTTATGTTGTTTTGAGAATGTTTTCTTAGCATCAAAAAGTCTTTGTTTAGCTCTTTCAATTTGCAATTTTTCAAAATAACTGTTATCTCGTGATTTAGCTTCATAAGCAGCTATTTTCAGTTTGTTTTTTAAGTTATTTTTTTGTTCGTTCAATGCATAGTAACGCTCAAAAAAACTTTTTATTACTTTGTTATTAAAAGTAAATAATTGATTGAAAAACATGCAATTAAAATCAACAAGTTCTCTTTCGTTTTTTTCAATTAAAGTTAAAAAATTATTGTATTCAAAATCAACTTGTTCAAAGTAGATTGAATTAATAAATTTTGATGTAAAAAACTGATTAATGACTTTACTAGTTTTTTGATTAACATTTTCAATTTTCAAAATTGAAAACCTAAATAAATCATTAAGCGCATTAAAACCTAAAAATCACTTTAACTGTTTAGGTATATTCACTAAATTAAAGTAATTTTGATCTTTTTTATAACTTAAATCTTGTTTTAGAAAAATGCTAAATGAATTAGGAAATAACCACTTATTTTTATTTCTTAAATCTATCTCTTCACATAAATTAAACTTAATTTTTTGAAGCGGTAAATATTGATGGTTATTAACAAGCAAAAACCCAGAATAGGGTTGTTTATTTAACTGTAAAACTTGTGAAAGGGTTTTTCATTGTGCTTGATTTAAATTATCAATGAGATAGTTACCATACTTAAATAATGATAGCTTTGCAACCCCAAAATAGTTAGCATCTAAAAAGGAGGTACGAATCATTAATAATGACAAATCAACCCACTGTTCTAGATTATCAAAAAGAGATATTTTCTTTAGCATGTACCTTTCAAATTTTAATTGCTAATCTTTAAGGTACTTAAATAGAAATAATAGTTGAAAGAATTAAGGGTGCTTTTGATGTCATCTTGGTGAATAAATTCATTACTGCTCGTTTAATAGAAACCTTCAATTCTTTTGAAATTTCTTTACCTATTCGTTTTTTAATCGTGGTGAAATCCTTAATATCATTAATTTGTTTTTTAATTAATTCTTCCATTTTTGTTTTTAGTTTATCTTTTTCTGGTACATCTAAAGATACTCCTAAAAAAGAATAAGTTATCTCATTTAAGAATTCACTCTTTTTTTGATCAAAATAAATGATTATGATCACAACTCCAGATTCTGACATTTGATCACGTTCAAACATAATGCTAGCTCCAATTTCTTGTAATCCAGCAGAATCAACACACTTAGGATTTAATTTCAATTCATTTTTTTTGCTATCTAGTTTTTTGTTTTCAATAGTTAAAACTTCACCGTTAAAAGGAATTAAGATTTGATTTTGCTCAGCGCCAGCTTGTTTTAAAACCATTGTGAAGTTTATAAAATCCCTATAAAGTCCACCAGTTGGAATAATATATTTTGGTTTTAAAGAACTAACTAAAAACTTCATATCTTCATCACTTGCTTGGATAGAAAGGATTTCTCTACCTAAGTTGTAATAACTAACTTCATTACGTGCAATATCATCAAGGATTTGTGCTTCTATCTCTTCATAGCCAGCAACTTTAGGAGTCATAAAGATAAAAGTATCACTCTTACGATAACGAATTCTTTCATCTTCGTTCATTCCAATTTTGAATAATTTAGCATACAACTTATCAGGTGGTGAGGTGAGAACAACAATACTGTTGGTTGAATTGTTAATCTCCTCAATAGAAATGGTATTTAAATGCGTGTTATTAAACAATTTTTGACGCACAATTGTGTTAAAGAGATGCACAAATGATTGTGAATAGATAATAAAAGGACGATTTTGCATCCTTGCAATCTGGGCAAGAGTCATAACACTGTAAGCATTTGAATCATAACAAGCTACAAATATTCTGCCTTTGGCTGGAGTTATGATCCTGTTTAACTGTTCTAATGACTTGTGTTTAGGTGTTGTAAAACCACTGTTTCTACCAACTAAACCAACTCCTGTTATTAATAACAATGTATTGTCAGAAAGTTTAGGGATAATTTGATTTAGTTGATTTTCAAAAGCAATGTTCTTATCATTTAAAACAATGAAATCATCAATAAAAACGATGTAACCATTATCAGTATTTAAAGCAAATCCAAATGATGATGGTAAAGAACTTGATACTTTAAAAGGGGTAATAGAATGATTAGAAATTTCGATTGTTTCAAGTGGTTTTAATTCATGAATTTCCAATTTATCACGTGCAATATTAAGTTTGTTTTCATTAATTTTGCTTTTAATTATAGAAGCGCCTATTGAACTTGTGTAAATAGGAAAAAATCCTACTGTATGGAACAAAAATTCTAATGATCCTAAGTTTTCAGTAATGGCATTACCTATAAAAATACCTTTAACTCTTGCTTGATTTTCTTGAATTCAACTAAAATCAGGAATAATTTTTTTAACACCTAAAACTGCTGTTGTTGGTGTTAAGCTACCAACATTAAAGATAAACACATCATTGTCAATTTCAAGCACATAGCAATTTTTACCACGTTCATCTTGACCGCCGAGTGCAAAGAATTTAATCTTAGCCATTTTTAACCACGGAGGTTTAAATTTTTAATCAAATCACGGTAGGTTTCAATATTACGTTCTTTTAGATATTTAAGTAGCCGTTTTCTTTTTGATACCTTTGTATATAAACCACGCTTAGAAATAAAATCCTTTTTGTTTGCTAACAGGTGGTCTGTTAATTTTTTAATCTGATCTGTTAATATAGAGATTTGTACTTGCACACTGCCAACATCGTTTTTGTGAAGTTGATGAGCCTTAATGATTTGTTCTTTATCAATTTTCATTAATTAATAAACACGTTGAATTATAACAAAATTAGAAAACGGCTCATTGCCAAGGTTAGAAGTAATAAATTCTCCTACTTACTTATTACTTATAATAAATATAGTTTTTATTTTTATTAATAAATTCTCTCGATATTTTCGATATTTAAATCTATACATCTAAACAAATTAACAAAGCCATTTAACTTATGGACTCAACCTTTCATGAGCTTGGGATCTCTCAAACTTTAATTGAAACGCTTAATGCGCTTCATATTAATAAGCCAACAAAAATTCAACAAATCTCTATCCCTCAGTTTTTATCAGAAAAAACTTAATAGTTCACTCGCCAACAGGAACTGGTAAAACTGCTGCTTTTGCAATTCCCATAATTGAGAAGCTATTAAAAGAAGATCAAACAGCAAAACCAACTTTAGTAATTGCTCCAACAAGAGAATTAGTAGAACAGATTAAAACCACATTTTCAAATATTGCTAAAAATAAAAAACTAAGAATTATTAGTTTAATTGGTGGTGTACCTGCTTGAAAACAAATCAAAAAAATCAAAACAAATCCCCAAATAATAGTTGGTACTATGGGTAGAATTATGGATCTTTTAGAGCGTAAAGCAATTCATTTTAGCGATTTAGAACACCTAATTATTGATGAAGTTGATTTAATGTTAGACCGTGGTTTTAAAAAACAAATTTTTAATTTACTAGAACAAATCAATTCCTTTAAACAAATTGCTGTTTATTCAGCTAGTTACAACCAAGAAGCTATTAACATTGCCAAGCAAATTACTAATAATGGGATCTTTATTGGATCACCTGAATTTAATAAAGACGCAAATACCAATAATGATAAACTAATCAACCAATTTGTTTGTTATCTATTTTCAGATCAAAAAAAGCAAGCTTTATACAGCCTTATAAAAACAGCACAAGTTAAGTCAATCATTGTTTTTTGTGACACTAAAAAACTAGTTGATGATCTTCATGTATTTTTAAGAAAAAATGAATTAAGAACTTTTGCACTTCATGGTGATAAAAAACAATTTATTAGAGAGAGAAATCTTAAAATCTTTGCCAATACAAAACAACCCACGATTCTAGTAACTACTGATCTTATTGGTCGTGGTATCCATGTTGAAGCAATCGATATGGTTATCAATTATTCAGCTTGTTTAAATCTAGAAGCTTATATAAATAGAATGGGAAGGACTGGCAGAAACAATCATAAAGGGACATGTGTAACTTTCTGCACCTCACAAGAAAAGAAAGTCTTTCTGAAAATGGTTGAGAAAATCACTGATAATCGAATAGCTGAATGTAAACAAATGGAAATAAAGTTAATTCCTTTAAAAAATAAAGCTAAAACTAAAAAAGGTGGTATTTCACTTGATTGTGTTCAGAAAATATATGCCAATGCAAAACCATATGACCGTAATAAACGTGTCCCTTTAGCAAGTGATCTTTTCAAAAGTCGTATGCGCCAGCCTGAAAAAGCTATGCAAAAGCAAAAAATTCATGACAATGACTGACAAAGTAATATGTAATAACAATTTATAAAATACTTGTATGGCAAAAAAAGACCAACTTACCTTAAGAGGGCCTTTGTATGGCAATAATCGTTCTCATTCCAAAACTATTACAAGAAGAAAATGGAATGTAAACCTTCAGTCATGCAAAATTAAAGATACTAATGGTAAAGTAACACGGATTCTAGTTTCAACTAAAACAATTCGTACCCTTAAAAAACAAAATCGTTTCTAATTTAAATTTAAGTTAAACTTAATCCATAAGCATATATGGATAAAAAATACGATATCACAGCTGTTTTGAACGATGATAGTTCTATTAATGCAGTAAGCGATAACTTCCAAATAACACTGGATGCACGACCAAAAGAAAAATCTAAAGGCATTAATCCTCTGTCAGCTTTTTTGGCTGGTTTAGCTGCATGTGAACTTGCAACTGCTAATGCGATGGCAGCTGCTAAGATGATAACTTTGAACAAGGCACTGATTAACATCAAAGGTTATCGTTTAACAAATCCTAGTGATGGTTATTTTGGCCTGCGTGAACTTAACATTCACTGAGAAATTCACTCTCCTAATGAGGAAGAAGAGATTAAAGAATTCATTGATTTTGTAAGTAAACGTTGTCCTGCTCATAACACTTTGCATGGAACTAGCAATTTTAAGATAAATATTAGCGTTACTTTAGTCCACTAAAACTTATTAAAAAAGCGAAAAATACCTGTAATTTTGCAGGTATTTTTTTATTTAAAAAAGGATAAATTTAAGAAATTAAATTTCGCTTTAGCAGTCGATAATTGATCATATGAAAAATAATATTAGTGATGTAAAGTTGGGACTGTTAGCAGCAAAAATTTATTGAAAATCTTGACGCTTTTTAGAGTTAACAGAAGATGACATTATCTCTATTGCACTTCATGCAGAGCAAGATTCTAAGAAGCGTTTTAATCCTGAATTTGGCTTAAGTTTTGACAACTATCTCAAATTAAATGGAGCAAATTTCATTAGATCAAGTTTTAGAAGTATGGTGAATAAAGTTGAATTGCTTGATTCTAAAAGTAAATACTCATTAGAAAAGCAAAACACAGTTCTAAATACACCTGAAAACTATTTACGGAGTTTAGAATTTAAAGAAATTATTACTAAAGCTTTTAATAAAGCTAAAAACGATCAAGAGAGAAAAGTTTTTTCTTTATATGTAAAAGGCTATAAAAACTTTGAGATAGCAAAAAAGCTCAATATTAGTCCTAGAAGAGTAAGATATTTATTAGATCTTTTTAAAAGCTACATCAAATTGTTAACAGAAAGATATGGATATTAAATAAAGATATTTTTCTTTTTAAAGAAATTTTCTACTAGTTTTCTAACAGCACTATCACTAGTTAATTTCAATGCTTTTTCAACTAATGATTTACATTCATTAATTGTTATTTTAGCAATAACCATTCTTGCTTTAAACATTGAACTTGCACTCATTGATAACTCTGTTAACCCTAAACCTAAAAGCAATGGTATTGCATATTGATCACTGGCCATTTCACCACACATTCCAGTTCAAACATTATTTAATTTACCACCTTCTACAACTAGTTTAATTAAGCGTAGTAATGCTGGATTTAATGGTTGGTATAGATAACTAACGTTTTTATTCATCCTATCAGCAGCAAAACTATATTGGATTAAATCATTGCTACCTATTGAGAAAAAATCAACATGTTTTCCTAAGCAATCAGCTGCTAATGCTGCTGATGGAATTTCAATCATTATTCCTAATTTAAATTTCTTAGTTTCATTAAATTCTTGTTGAACTTTTGTTAAAAGTTGCTTAACTTGAACTAATTCATCAAGAGTTGCAACCATTGGAAACATAATTCCTAAATTTCCATAATCAGAAGCTCTTAACAAAGCACGAAGTTGGGTCTTAAAAACAGCTTGTTTATCCAATGTTAAACGGATAGCACGATAACCTAAGAAAGGGTTATCTTCATGAGGAAATTGAAAATAATTTAGTTTTTTATCTCCACCAATGTCTAATGTTCTAATTATTACCAAATCATTTTTAGCTTTTTGTAAAACAGTTTTATAAGCTTCAAATTGAACTGATTCATCAGGCCAATCTTGACTACTCATATAGAGAAATTCAGTTCTAAAAAGACCAATTCCATTCGTGTTATACTCAACTGCTAAATCCATATCTTTTACATTACCAATATTAGAAGCGACAATAACTTCATATCCATCTAAGGTTTTTACTAATTTATTGGTATATTGTTTTAATTCATTTTGAAAGTTGGATTCCAACTCTTTTTCTTGTTTTCATTGAGTTATGTCTTTACTTGAAAAGTCAAAACCCACAATACCTTTTCTACCATTGATACCAACTGTTTTGCCATCTTCAACTTTACTGGTAATATTTTTTAAACCAACTATGGCAGGAATTTCCATAGAACGAGCCATAATGGCAGCATGACTTGTTCTGCCACCACTCTCAGTTAAAAAACCCTTGACATACTTTTTATTAAGAGTTGCTGTTTGACTTGGTGTTAAATCATTAGCTACTATAATGACATCACTTTTAATCCTGATTAAATCATTAAGTTTTACTCCAGTTAAATAACTTAAGAGCCTTTGGTGTAAATCTAATATATCACTGGCACGTTCTTTAAAATACTTATCATCCATTTCACTAAACATTAAAGCTGTTTGTTGAAAAACATTATCAACAGCAATAACAGGATGGATGTTTTTATTTAGTTGTTGTTCTAATTGCTCAGTGATAGTAGGATCATTAAGAATTTGGATGTGTGCATCAAAAATCATTCCTGCTTCTTGATTAATATTCTTAACAGTAATTGTCTTAATCTCTTCAAGGTCTTTTTTTGCTTTTTGAAAAGCACTGCTTAAAAGCTTTTTTGCTTGGGTGGGTGTCAATTTGACATTTGTGTATTTTTTAACGTCAAATTGAGGTGTTTGAATAATGAAAGCTTTTGCAACAGCAATGCCATCTGAAACACCAATCCCAATTATTTTTTTCATAGGTTAATTGTTATTTTTTAATAAGGATTTTGCAGTCATCTCTTTTGGTTTGCTAAGGTTAAGATATTCCAAGATAGTAGGAGCAATATTAGCTAAAATTCCAGTTTGATTAAAGTTAACATTTTTGTCAGTACATACAAATGGTACAGGATTAATAGTGTGTTTAGTAACTGGATTGTTATTATTATCAATCATCACTTCTGCATTCCCATGATCTGCAGTTAAAAACATAGTTATTTGATTAGCTTTACAAAAATCAACTATTCGTTTAATTTGAACATCGAGTGCTTCAAGAGCTTTAATGCAAGCTTGATAGTTACCAGTATGACCTACCATATCAGGATTAGCAAAATTTAAAACAGTAAAATCAAAGTTATTAAGCTTTTCTAGTAGTGCATCAGTAATAGCTTTACATGACATTTCGGGAGCTAAATCATATGTAACAACTTTTAAAGAAGGAATTAATGTCTTTGTTTCATTGCTGAGATTAACTTCAAAACCACCATCAAAAAAGAAAGTAACGTGAGCATACTTTTCAGTTTCTGCAATCCTCAATTGCTTCAAATTATTATTAGCAATTACTTCACCAAGACTATTTTTAATGGTTTGAGGTGGAAAAGCAACTTCGCTAGGTACAATTCCCTCATAATTCATCATTGTTACAAAAAATAAATTTTCTTTTCGTTTCAATTCAGGTTGATAGTTGTAATAATTGCTGTTAAAGATCAAATGGGACATTTGTCTTGCTCTATCAGGTCTAAAATTAAAGAAAATAACTCCATCATTATTGTTTAATGCAAACTGATCAGAATTTAAATTGGCATTAATTGCAGGATAAATAAATTCATCAGTAATTTGGTTCTGATATTGCGTTTCAATATAACCAATTGGGTCATTGAATTTATTTTTTGAAACTCCTAATAAAGCTTTATAAGCAATAATTTCACGATCCCAGCGTTGATCACGATCCATTCCATAGTATCTTCCCCCAATAGTTCCAATAACAACATTAGGATAGTTTTTTAGAAATATCATTAATTTCTCAAGATCTTGTTTTAAGCTACAAGGTGCTACATCTCTACCATCACCAAATAAATGTAATACTACCTTTGCATGTTTTGAAAACAATTCAATGAGTGCTAATAGATGTTCATTATGACTATGCACTCCTCCATTGGAAAATAACCCAATTAAATGGATTTTTGAATGGTTTTTTTCTACATGTTCTATGGTTTTTAAAAAAGCTTTATTTGCAAAAAAACTACGATCCTTAATATGTTGATTAATCAAAGAAAGTCCAGTATAAACAACTCGACCTGCCCCAATATTTAGATGACCTACCTCAGAGTTACCTATTTGACCCATAGGCAATCCAACTGCTTCCCCAGATGCATCTAAAAGTACACAAGGATATGAATTGATTAATTCATCTAGCATTGGGGTATTTGCATTTTGTACTGCATTACCATAAATAGCATTTGAGATCCCATAACCATCAAGGATTGCTAATAAAACTTTTTTATGCATATACCCTAGCCATTTCTAAAAAGTTTTTAATTTCTAAAGATGCTTTACCAACTAAAAATCCATCAATTTGTTCCATTATTGCTAGTTTTTGGATATTATTATGATCAACTGATCCGCCATATAGAATTGAGATATTGTTAGCAACATTTTCATCATATAAGTCATTAATATATTGCCTAATGGTTTTAATGGTTTGATTTGCAACTTCAGGAGTTGCTGTTTTACCTGTTCCAATTGCTCACAAAGGCTCATAAGCAATAACTAAATTTTTAATTAAGCTTTTGTCAATCGTATTTAAGCAATTAGTAAGATCAGTTTTAAGAAAGCTAATCTCTTGTCCTAAAGCCTCACCAATACATAAAACTACTTGCATGGATGCTTTTAGACAAGCAAAGAGCTTTTGATTAATAACTGCACTGGTTTCGTTATAGTATTTTCTTCTTTCAGAATGACCAATAATACTGTTGTTAACACCAATGTCTTGAAGTTGAGTAAAGCTTACAGTTCCAGTATATGAACCACTTTCAATAAAGTTAGCGTCTTGGGCAAATAAAAGGAGACTATCACTAATTATTTTTTTTATTTCAGTGAGATGAACATAAACAGATGCTATCCCAATTTTGGCATTGTAATTAAGTTTATTTTGTTGAAATTGTTCAACAAAACTAACTGCGTCTTTTAAATTTTTATTTGTTTTCCAATTGCCAATTAAATACCTTGTGCGCATATGAAAGATTTTATAAGTTTTCAAAAACCATTTTCTTTTCCAACTAATTGATCATTAAGTTATGACTTGTTTTCAGTTCCATGTTTTGTTTGCTTATTGGCACGTCTATCATAGATATAGCCATCAAGCTTTTTAATAAAAGTAATGGAAAACAAAGCATCACGATCAAACTTTCTCACATCTTCTAAAAGCTTAGCAGCTTTAATAAGCAAACAATTAGTTACAAGCACTTGGGTTTTTTGGCGTGAATATCCTCCTTCAGCTTCAAACATTGTGATTGAAAATCACTGTTGATTATCAAGTAGATAGTTACGAATTTTTTCTATGTGCTTACCATAAACTTCTACTTTTACAAATTGATACTTAGGGAAATAAAAAGAAGTAAATAATCCCAACACAATGTTCATCAATAGAGTAAAAACAAGGTTTGGAGATAAAAAGAAAGCAACTCCAAATGGCTGGTGTTTATCATCACCAACATAAGATTGCACTGATAAACTACCAGTTAAATAAGTACCAATTACATAGCCAATAATAAAGCTAACTGTATTAATTAGCATCAAAATTCCACCAATGTCTTTGTATTTTTTTTCAGAATATCAGAATGCTAAAAAGTCAAGACCGCCTGTAGAAGCGTCAATAATTAAAATAACTGAATAAAAAACTGCTTGTAAAAACCCCCAAATAAGTCCATAAAAAAGTAAAGATACTTGTTTTTCAGCAGATTTTTCTCAAAATATAAGTTGTACTCCCTTTTCATTAATTAAATTTTCAAATCCACCATCTTTTGCTGTAGTTAAATTTGCAAACAAAAAGAAGTTATCTATTCCAGGGATGTATGAAAAGAAAAAACCAAAAAGATTTGAAACAGCTACAAAATAAAGGGTTAATAGGGTAAATTTTTTGGAGATTTTAAACCATCCAAAAATAAAGAAAGGAACATTAAAAAGAATTTGTGTTAACCAAAAAATAGCATTAAATATAGTTGCTGAATCAACATTAATATTTTGTGAAGTAATAAAAAAATTAACAAGACGAGCTAATCCTTGGCTAATGGAAGCCATACCAATGTCATAAAGTCCTGAAAACTGAACAAAAATAACACCAAGTAATCCTCAAAAAAAAGCAACTATTGTCAAAATTACAAGTTGTAAATAAAGCTTTTTAAGGTTGTATATAGATTGAAACTTAAGAAATGATCCAGAAAGATGAATCCTTACAAAATTACCTGAAATTTTGATGTTTTTATCTTTCATAGTTAAGAACAATTATTTTAATAATTTTGGTAATTAATAATTTCTTATTTACTATCTTATTAGTAATATTAAGCTTAGTGCAATAATGGCAACGAAAATAGAGCTAATAAAAGAATTGCGTAAATCAACACAAGCAAGTGTTATGGATTGTAAACAAGCTTTGGAAAAAAATAATGATGATTTTGAGAAAGCTGTTAAGTGATTAAGAGAAAATGGCATTGTTAAATCAACCAAAAAATTAAATAAGGTTGCAAGTGAAGGAATTATTGTTTTAAAAAGCAATTTACACAAGGCAATTATGGTTGAGATAAACTCACAAACTGATTTTGTAGCCAAAAATCAAGAGTTAAAAGAATTTTCAGATTTAATGCTTGAAAAAATATTTGAAAAAGTAAATCCAAAAACAGAATTAGTTGAAATTGAAAAAATTCAAATTAATAATGATGAAAAAGTTAGTGAAAAACTAGCATTAATTGCTTCTAAAACTGATGAGAAAATAGTACTTAGAAGAGTAGTTGTATTTGAAACTAAAACTAATCAAATTTTCACCTATTTACATGCCAATAAAAGAATTGGGGTAATTATTGAGATTCAAGGAAAACTCAACGAAGATGATGGTAAGCATTTAGCAATGCATATTGCTGCTAATTCACCACAATTTATTGATCAAAGTGATGTTAATCAAACATGACTTCAAAATGAAAGAAATATTATCCGTTCCCAAGCAGAATTAGAGGTTAAAGAAAATCCTAAAAAAGCAATTTTTTTAGAAAAAACTATTGAAGGTAGAGTTAACAAATTACTAATTGATACCTGCTTAATTAACCAAAAATACTTAATTGATGAAACTAAAACAATTGGTCAATTTTTAAAAGAAAAACAAGCTAAGGTTCTTAAATTTATTAGGTATGAAGTGGGAGAGGGGATTATAAAGGAAACTGTTGATTTTGTTAGTGAAGTAAATGCACAAATCAAACAATAAAATCCGCCAAAGAATAATCATTAAACTTAGTGGTGCTGGGCTAACCAAAGAAAATTCTCAACCCTTTTCTAATGATTTTTTTGAAACTATTATTAATCAATTAAAAGTTTTAAAAGAAAGCTATCAAGTAGGAATTGTTATTGGTGGGGGTAACATTATCAGAGGTAATAATTGCCAAGAATTTAACATTGCTGAATACCATGGTCATCAACTTGGTATTATAGCAACAGTAGTTAATGGCTATTTTTTAAAAGCAAAGTTAGATGCACATAATTTGAAAAGTGCTTTACTAAGTGCAATTAGTTGTCCTAGTTTAGCAGTGCAAATTCTTTCACAGCAAACTATTGATAAAGCTTTTGAAGAGAATGACTTTGTCATTTTTTCAGGTGGCACTGGTAATCCTTATTTTTCCACTGACACTGCATTAGCTTTAAGAGCAGTGCAAACAAAAGCAGTTGCTATTCTGATTGGAAAAAATGGTGTTGATGGTGTTTATACAGCTGATCCTAAAAAAGATAAAAATGCAACCTTTTTACCAACACTCAACTATGACCATGCCATTAAAAATGATTTGAAAATTATGGATATTACTGCTTTTACTATGTGTAAGGAAAATAATCTGAAAATAATTATTTTTAACATTAATACTGAGAATGCATTATTAGATGCATTAAACAAAAAAGGTCGCTTTACTATAATTGAAAATAACTAATGACAAAAGCACATTACATTGATTTTTTTAAACAAGCAGCTGATAAAAAAATTCAATGATTAAAAGAAGAGTTAACAAAGATTAGAACAGGTAGGCCAAATCCTAAAATCTTTGATAATCTTTTGATTGAAAGTTATGGACAAAAAATGCCTTTAATATCTTTAGCTCAAGTGACTATTAATCCGCCAAGAGAAATAATCATAAAACCATTTGATCCTAAGAGTAATACTAATGCTATTTACAGTGAAATTCAGCGGGCAAACATTGGTGTTCAACCAGTTATTGATGGTGAAAAAATTCGTGTTAATTTTCCCCAAATTACTCAAGAAACTCGCTTAGAAAATATTAAGCACGTTAAAAAAATAATAGAGCAAATTTATCAAGAACTGAGGGTTGTAAGAAGAGATGCATTACAAATGATTAAAAAAGATAATCACAATGAGGATTTAGAAAACTCTTTAAAAGCTGAAATAGAAAAAATTAACAAAAATTATTCTAATCAATTAGAAGAGATTCAAAAAGACAAAGAAAAAGAATTGCTAACAATTTAAATGAATGAAAAAGCAAAACAATTCATCAAAAAGCGAACTTCAGTATTCATTGCTTTATTAGTTGTATTTTGCTTTTTTCTTTTAATTAGCGCATTTGCTGATGGTTTTAACTTTTGATCACCGTGATCAGCAGATTTCAATTCAAGAACATTAAAAGTAGAACAAGCAAGTGGTGTTACTAGTGTTATTAGTACTGAGATTAATGAAAACTTTAAAGCTGTTCGTTTCAGCTTTAGCATAATCATTATTTTAATTGTTGGGGTAATTGGTTCTCTGATGATTTGAGAGTTGTTCACAAACATACTAAAAAATAAACCAAAACTAAGCTTAAGTTTAACGTTGTTAAATGCTGGAATAATTATTTTTGGGATGATTGGTACTTTTGTTGTTGTTTATTTTTACAAATGAAATGCAACTGTTAATGGTATTTGAACATTAAGTTTTACTCTTTCTGTGGTTTTACTTTGAATAATTTACATTGCTTGCATGAGTAAAACAAGAATTAAGTTTAGCTTACAACTTTCATATAGCTTAGGAGCTATTGCTTGCTTTATTGCTAGCATAGGTACTATTTACTTTTCTGTTATCAGGGGTTGAACTACAATCTTTTTATTGATGAGTTTAGCAGTCAGTGTTGATACATTTTCTTTTCTTTTTGGAAAGTGCTTTGGTAAAAATCCTTTAATTAAAATTTCACCATCAAAAACATGAGAAGGAGCTTTTTTTGGCATCATTAGCACCATTGTTGTTGTTGCTTTACTTTGTGTTTTATATTCAATTCCTTTCTTTGTAGCAAAGCCTACTTTTAATCAAACAAATGGAATAGCGCTCAATACACCCCAAAATTATGATAGCCATAATCTTATTACCAATATTTTTTTAATTGCCTTTATCTCTGGAGGAAGTAGTTTTTATATCTACTGGTGGGTAAGCACTTTAGCTTTAATTTTTACAGGATCTGTTTTTGCAATAGGCGGTGATCTTTTTTTTAGTTATATTAAACGCTTAATTAGTATCAAAGATTTTTCTAAGGTTTTAGGTAAACATGGGGGAGTTTTAGATCGATTTGATTCAAGTTCTTTTTTAATTAGTTTCTTCTTTGTTTATCATTTAATAGCAGGAACCATTTCCAACCAAAGGTTGTTGATGGAACCTAATACTTATTTCAGTGCAATCACTAGTATTCAAAGCTAGTATTTAGAATTAATAAAGTATGACTCCAAAACTAAAGCTAAATAACAACATAAACTGAACCAAAAGAACAATTGATTCTTTGTTTGATTTAAAAAAAGGTGAAATGCTTGAAAAAGAGTTAATTACACCTGAGGGAAAATATGAATATTTTAACGGTGGTGTAAAAAATTCAGGAAGAACTGACAAGTTCAATACTTTTAAGAACACTATTAGTGTAATTGTTGGTGGATCTTGTGGTTATGTAAGGCTAGCTGACAAAAATTTCTTTTGTGGTCAAAGTAATTGCACATTAAATTTGTTAGATCCGCTTGAACTTGATCTTAAATTTGCTTACTATGCTTTGAAATCACAACAAGAAAGAATAGAGGCTTTAGCTTTTGGGACCACGATTCAAAACATTAGAATTTCAGATTTAAAAGAATTGGAAATTCCTTTCACATCAAATAAGAATGAACAGCATGCTATTGCAAATACTTTAAGTGTTTTTGATGAGAGACTAGAAAACTTAGCTTCTTTAATTGAGATTAACAGGAAACTAAGAGATGAATATGCTCACAAACTCTTTAGCTTAGATGAAGCTTTTCTAAGTCATTGAAAACTAGAAGCATTACAAAGCCAAATGCATGAAATTACTTTAGGGGAAATATTTAATTTCAAAAGTGGTAAATATCTAAAGAGCGAGGAGAGATTAGAAGAAGGGAAATTTCCTTATTATGGAGCGGGAATTGATAACACTGGTTTTGTAGCTGAACCTAATACTGAAAAAGACACAATTTCTATTATTTCTAATGGTTATTCACTAGGTAACATCAGGTATCATGAAATTCCTTGGTTTAATGGCACAGGTAGCATTGCATTAGAACCCATGAATAATGAAATCTATGTACCATTCTTTTACTGTGCTTTAAAGTATTTGCAAAAAGATATTAAAGAAAGAATGAAAAGTGATGATTCACCTTTTTTATCCTTAAAACTAGCAGGTGAAATTAAAGTGCCTTATGTTAAGTCATTTCAACTGCAAAGAAAGGCAGGAAAAATCGTCTTTTTGTTAGATCAAAAATTAGACCAATATAAAAAAGAACTAAGTTCTTTAACAGTGATTCGTGACACTTTGTTAAAAAAATTATTCCCTGATATGACTGAAAGAACTAAATCTATTAAGGATTATTAATCAAAAACTTAATTCTTTCTTTCTAAAATTACTTTAATATCTCTTTTCTTGCAAAATTAGATAATCTTTTCACACCAATTGAATAACCTGATTCAAAATATCCACCATTATTTTGATAGATAAAATTAACAAATACAAAAATTTACTTTCTTTATTTTTAATTTATCGATAAACAGTTCTTACAGCGCCAATATTAGTAACACTTTTTTTGGTTTAGCATTTACTTAATTTTTAGTTTCTTTAACAAATTGAATTGTTATAAAAATGCGATTATTTTCTTGGATTTTAACTGCTTAACTTTTTTTATTTTGATATAAAGTTAACTAATAGAAAAGTATTTATCTTCTTTAGTTTTAAGATCAAAATATATCAGGTTTTATTTTTAAAAAAATTAAAAAATCTTATTAAAGCAATGTGTAATAAAAAAACGGTAAAAGATCGATTGACTTTCCCTTAAAAGTTCTTGTTCTAATTTTCTTCACTTGTTCATTTGTTGAAAGTAATAAATTCAACTTTTCATGAGAAGTACTCATGTCAATTTTGATATCAAAAGGAAAGTTAATTGAAGCAGGTATACTACTAAAAGTACTTTCACTTACCCTCCTATTATCTGTTGGAGGAGTATAAGTAAAGATAACATCACCATCAAAATTAGCTTTATCACCAGTAAAATCACTTTCACTTACACCACTTGTACTAGCTAAAAAGGATTTGATTAATGAAGTACTAGTATTGTCACTACCAACTATACCAGTCATTTTTAAGTGAATTTGAACTTTAAAATCTAAGTTATTTTTTCAACTGTATTGAAAATTAACTACCTTTTGACTTTTAACTCCTAAAACTAAGGCGATTTGACCACTTATACCTGGATAAGTTTCATCAAAATATAAACCTTTTGCACTGATGTTAGGAGTAACATCTTCCTTTGATTTATTAACTCCAAATGCTTTAGCAAATCTGCTATAATCAAGTGGTTTTTCTAATAGTTGATAATCTAATAAAGTTGTTTTTCAACTGGCAAATACAGTATTAATTACCTCTTCCTTATCAGAACTATAACCCTTTTTTAAAAAAGTAACAGCATTATTTTTTGAAAGTTCTATATTGTTTCTCTGAAAATAAGCTCGTGCTTTTTCTAAACCAGGATCAGTTGATGAACAGGCTACAGATAAAGTACTAAGTGAAATTAATGGTAATAAAAAGATCTTTCTGAGTTTCATTACAAAATGGAATAAAGCACATATGGTAAAAGATCGATAGTTTGATCTTGGAAGTTTTGATCAGCTAATTGACCTACAAAATTACTTTGTAATAACTTTTGCATCAATTCATCTTTACCAATAATTTGTAACCTTAAATTGGTATTAAAATTAATTTTTCTCAATAGAGTATCAAAACTAGATTTGGAAAATAAATTACTGGAAGGTGGAGTATAAGTAAAAATTAAATCAGCTGAAATAGAATTTTTATCTTTTATATCACTATCACTTAATCCTATTTGACTCAAATATGATTTTGCTTGAGCACTGTCAAAAGTTCCAGTAGCTTGTAACCTTATTTGGACTTTGAAATCACGAGAACTGTTATAGTAAAAACTAAAATCTGTAACTGTTTGACTACTCAATTTAATTACATTATTAATAATACTTGCAACATTTTGCGTGTATCTTTCAACTAAACGTAAGCCTTTAACACCAGTGCTTGGTTCAACATCTTCCTTACTCTTACCACTGCCAAATGCTTTTACAAATCTACTTGGGTCTTGTTTTTCAAGAATCTTTTCGTCTTGCAAACTAAATCTTCAAGCATCTAATAGAACGTTTGTAGCACTTTTTGAATCCACTTCAAAACTATCTCTTAGTGAAGTAACTAACTCTTTCTTGTCTTGATTTAATTCACTATTTTTACTGAAACTATTTCTAAGTTGGTTTAAACTGGAAAAATTTGTTCCACTGCTTAAGTTAGAGCATGCTGTAATTAAAGAACCTAATACCATTAAAGAAGAAATGATGCCAGCTTTTGTTCAAAATTGTTTTTTTAACATTGTTTAAGTTAACAGATTTTCTTATTTGTAATTTTTTAATCATTAATAGAGGTAAACTTCAACGACAATCATTTAGAAGTTTTGTTTCTATTTTTATCAATTAACAAATAGAAGGTTTCTATACTTAAAAAAACAAAAATAATCACTTAATTTTGAATTAATAACTTCTTGTTCTTGCAAGCCTAGATTATTGACATGAAACAGTTTTACTGCTTGAAAAGTTTCATTGGAAATCGATTCTCTACTTAATCTAATTCCAAGATTTTGAATTTGTGTTTTTATAAAAAAATGAATATTAGCAATTAAGTTTCAAACTATTTCTGTTTTTTGAAAAAGTCTAACTAAAAGATATAAAGAAGTCTGATTAAACAAAAAATGCTTAAATCTTTTTCCAAAGTTTCTAAATAAAACTAATATTATTTTTAAACAAAAAAGAAACAGTACAAAAAATGCAAATACTGATGCTTTTAAACTAAATTTAGATCTAAGAAAACTAACACTCATTGCGTTTTAAGCAAACTAAAAACAACAATTAAGAATGATTTTTCAAAAGTTGCAACTATAATTTTAAAACTTTTATTGATTTCTTTTGTATTAAAAACATTTGCACAACTTACTCTAAATTAATATCTTTTTACTAATAATAAGAGATTTTTTTAAAACTAATGGAATTACTAATTAAGTTCATAACTTAATTTGCCATATTTCCCATTAATAAGTTCATTAATAAAAATTTCACATGCTAAGTTAGTGTTTAGTTCATTAGCTTTCTTAATTAAGCCTCTCACTTTAGCAAATTTTTCCAAAAAGTTAATAAAGCTATCCGCTTCAAATGGTAAAAGCTGTTTGTAATGTTTCTTAAGATAATTAAAAGCAAACATTCCCACTTCTTCTATATTAACTACTTCTCTTCTAATAACATTAGTCAAAACTAGTTTATATCCAATTTGAATTTCATCAATTCTTTTCAAAAAAACTCCTGGGGTATCACTTAATAATAATTCAGGGCTAATTTGAATCCAATTTAATGATTTAGTAATACCAGCGCGGTTAGCTACTTTTAAGTGATTTTTATTTATCAAAAGGTTAATTAAACTAGATTTACCTACATTGGGCATGCCAATAACTGCTAATCTAAATTGTTTGATTAATAAGCCTTTTGCTTTTAATTGTTGTCTTTTATTTGCAAATAAAGTTGTTAATGTCTTTAGAACTTTTTTTTTGAGTTTAAAAGGTTCTTTTAATGAACCAAATAAGATCTTTTTATTTGGTTTGTATTGTGCTAAATCTGTCTTTAATGCCAGTATTAATTTTGGTTTATTTAAAAAATAACTAATTATTTCTGAATTATGAGTTAAAGTTGGTGCTCTAGCATCTACTATCTCAATGATGCCATCAATTTGACTAGATAACTTTTTTAATTGATCATGGGTCTTTTTCATGTGACCCGGGAACCAATTAATTTTGGCGGAGGTATATGTGTCCATCTATTCCCCTGATTCTTGTAATAGATACCAAACAATCCTTGTCAATTTGTCTAATAATCTTAATTAAATGGGGAATTTCTACATACATGGAAACAGAAACTATCATCTTCTTTTTGAGTAAAGAATAACCACCAAGCGTTTCTTGAATAGATAAACTGTGATTAGCATTATCACTTAACAATGCTTTTCTAACTTCTTCAAGCTTATCAGTAAATACTTTAATTTCAGCAAAATTATAACGAGGGAAAAGATAACTAACTACTGTTCCTGTTAACAAAATTGAAAAAAAAGTTGCAATTAAATTAGGTGAGAAAAATAAACTAACTTCCCAAGCAGAATCACGATAATTATTGACATCTTGCAACAATAAACTACCAGCAACAAAAGAACCAATTAGAATAGCAATAATCAGAATAAAACTATTTACATAAAAAAGAATTGATCCTACTGATCTATTTTTCTTACGAGCATAATATTGGGTTAAGAAGTCCGCTCCCCCAGCAGAACCACCTAGTATGTAAAGTAATGAAACTGATATGCCATTATAAAAACCATAAATAGCTGCATAAATAAATGTGGAAATAATTACATTACCTTGGGAAGTATCACTTCATAAAAAAGGGACAAAACCGGCACTTTGATTTAAATCTTTCGTTGCTTTTCAAAAGTTAGTATCTGTTATAGAAGCTAACATTGGTGGTAAATTATCAGAACCTGGAATAATACTGATTAAAAAACCAAATACGTTAGATGCAACAACAAAATGGGTTGAAAGAATAGTAAAGTTTTTACCTATTTTTTTATAAGAAAAAATAATTAAAGGGATGTTGATAAAGACATATAAAAGCCAGTAAAGGATGTTAAAAATTAATAATCTTTGAGTTTCATCAAAACTTTTTAACAAAACAAACACTAATCTAGCAAAACCTTGAGTTAAAGAACTAATTCCAAAGCTATATAAACCTGTTTTTTGAATAAAAATAAGTAAAAGAAAAGCATTAATAATTGATAGTAAGTAAACTAATGCATACTTTAATGGTTTTTTAGCCTCATAAAGATTGCTAAACATCAAAAAAGAGTTAGAAATCCTTACGCGTTTAGAACCAGATGCGACTGTTATCTTTGATTCTTTTTTAAATAAGTTGTTAAAAAATTTCATTTGTAACAAACTTTTTTAAGACTTTTTTTCTTTAATTTTTGCTGACTTACCAGATCTTTCACGCATATAAGAGATGTATGCTCTTCTTACTTTACCCCTGCGTTTTACTTCTATGTCTATATTAGGGTTGTGGATTTGAAAGTTTTTTTCAATAGGAATTCCATCAGTGGTTTTTCTTACCATGAAAGTTTCGCTAATCCCTCTTCCCCTTCTTCTTAAAACAGTTCCAGTGAAGTTTTGAACTCGAACTTTTTCTTTTTCACGTAACTTAATAGCAACATTAACTTCATCTCCTGCTCCAAATTCAGGAACATATTCCTTTAACTGTTTTTGTTCTACTGCATCAATTAATGCTTGTTTATTTATTTTTTTCATTTTTATTTTCTAAGTATTTTTTGTATAAATCAGGTCTGTATTTTGCAGTTTTTAAGATTTGCTGTTCTTTACGAAATGATTCAATCTTTTGGTGATCTCCTGAAAGTAAAACTTCAGGAACTTTATCGCCTTTTAAATCGTATGGCCTTGTATAAACAGGAAAATCTAATAGATTATCATTGAATGATTCACAAATAAGACTCTGATCATTAATAACTCCTTTAATTAATCTAACAGTAGCATCAATAACACTTAGTGCTACAAGTTCCCCACCACTTAAAACAAAATCACCTAAAGAAACAATTTGATCAATATATTTATAAATTCTTTGATCAAAACCTTCATAGTGACCAGATAACAAAATTAAGTGTTCGTATTTTGTAAGTTTTTTAGCACAATTCTGAGAAAATTGTTCACCTTCTGGGGAGAGTAAAACTACATGAGAATTTGGGGCTTTATAAAAATTTAAACAATTAATAATAGGTTCAGCTTTTAAAACCATGCCACTTCCTCCACCATAAGCCATATCATCCACAGTTTTATGTTTATCATTGCAAAAATTTCTTCAATTTACTACTTCAAATTGAACTAAATTTGCTTTTTGAGCTTGTAACATAATAGAACTATTTAAGTAAGGCCAAATAGTGTTTTCAAAAAGTGTTAAAACAGTGATTTTCACTACTTTTTAGGACTTGTTTCTTTCTTATTTTTACTTTCAATAAATTTCTTCCACAAACCAGATTCACTAAATAAAGAACGGACTGTATCAGTTGGAATTGCCCCTTTATTAAGTCAATCTAAGGCAACAGTTTCATCTAATTTACACTTATTTTCCTTTAAAGCTGGATTTAGATGTCCAATTAAAGCGATATACTTTCCATTACGCTTTACTCGCGAATCAACCGCTACTATTCTATAAAGCGGATAGTGTACTCTTCCCATCCGCATCAATCTTATTTTGACCAATGATTTTTTTCTTAATAAAGCGGCTAATTATAACTTTTTAAAGTTCTATTTTATCTATGCTTAAGAATAACTTAATCAGCTATTTTTTATTCGTCTAGTTAATAAATTTAATCATCCAATAAGGCCCGATTTTCATTTTGCATGTCATCTGCAAACTCATTATTTTTTTAGATAAATAAGTTCTTATCTTTATATCTAACAAAGTCTTAATTATTTTCTGTCGTTTAGCATATTATTTTAAGGCGTTAAACTTGTTTTCAGAATTAATGATGAAATAGTTGTTTTGCTATTCACAATAGCAAAACTTAAATTTGAATTATGTTTTTGTTTATTAATCTGTTCAATATGTTCTTCTCAAATTAACCTATATTTTTATCGGTTAATTCTTTTTTAATTTCATATAAGTAGATTAATGTAGTTCCACTATTAACAAATTTTTGTTTATTTTTAAGGAATTCCAATAGTTTTTGTCACTTTCATTTGGGAGGAGAGATTTACCATTTTTAATTGAATTAATTACAAAATTTTTTTAACAAATAATTAGTTGTTTAATAATCGAAATTATTAATTAGTTTTTTAGCATTCATACTGTAATTGGATTTTTCTAATTATGAGTACTTTTCAATAAATTTGACCAACTTATTTTGTGGTGGATTAGCGAAATATTTATCCATAAACAGATTAGTGTGAATTTGACCAAAAGCAATATTAATTAAACCTGCTAAGGCAGCATTTGCAGTTAACAATCAAATAAAAACTTCAATGTTATTTGTTTGAAGTGTTATTGCTTGAAAAATAAAAAGTAAAGGAATAAATACAAACAATCCGTGTGTTAAAGAGACAATTCAAGAATAAAGCACTCTGTTAGTATTTTGAAAATATTGTTGACTATTAGCGCCTGTGGCTACAAAGAAAACTTGTGCTTGCACTATTAATGAAAAGTAATTTGCTAAATCCAAATTTTGATCTGAAACATCAAAAAAACTAGATAAGATCTGTTTTCCAAAAGCAACTGCTGTTAACAAATAGATTAAACTACCGAACGATATACAGATAATAACTGTATAAATGTTAATTTTTTTAATTTCATCATATTTTTTTTGTCCAAACTTATATGATGAAACTGTTCTAACTCCTTGTAATAAACCAAAAATAGCTGCTGAAGCTAAATTACTAATAGCAATAGGTCCAGTTAAAAGTGTCAGATAAAAAACATCATTTTTATCAGTAGTTGCTTTTGTCAAATTAACCAAAAAACTCTCATAAAATGTAGTAACAATAGAAAGTGATCCATTTCTAAAAAAGGATGCCATTCCTATTAATGAAACTACAACTAGTAGATTAAAGTCAATTTTGTTTAATTTAATAGTTTTAAAAGTTAAATAAGTAAGATTTCTTTTGTTTAAATAAATTAGATAAATAATATAAGCTAAAAAATTAATTAAGTATCCTAGTATTCCAGCCACAGCTGATCCTATTACTCCTAAAGAAGAATATCTAACTAATAAAAAAACTATTAATATGTTAATCAAATTAGCAATAGGCGGAACAATTGCAATAAAAAGTTGTCTTCCCTCTGATTGTGCTAAATAAAAAAACAACCTTGAAAGCATAGGAATGATGTTAAGACCAATCAAAATATAAACATATTCAGATGCTACATCAATAGCCTTTTTGTTAAAAAACTGTTGGAAGCTATTAGCTTGAAAATTTTGTTCAAAACTTGATTAATCAAGATTATAATGCAACCATTCTTTTGCAAAAGATAAAACCAAAAATTGGGTAATTAGTCCAAATACAGTTGTTGATATTAATCCGGTATTTCAAGCTTCCTGAATTTTATTTTGATCATTTCTACCAATTGCTTTAGAAAAAATAACTCCAGTTCCTAGAGGAATAAAAATATTAATGGCGTTTAACAAAACAATCAAAGGTTGACTAATATTTAAAACAGTTTTAATAAATTGTTTAATATTAAGTCCAGAGCTTAAAAAACTATCACTTTTATAAAAAGCAGAATTTTTAAATTCATCAATTAAACTCTGATCAGTAAAAATAGAATCAGGATTAAGTTCACTTCTAGGAACAAACTTAATCACCATGATTTGATCTATAAATACATAAGCAGCACTAAAAAGTGCAAAGAAAAAAGTTGGAACAGTAAATCTAAGAATAGTTAGAAAAAGCTGTTTTGATTCAAAAACATTTTTAATTAGGAGTTGTTTTTCAAAACGATCCCTTTTACTGTTCACTTATTTATTTTTAAAAAGATGGATGTAATCCTTGTATCCTAATTTTTCCATCTCTTCAAAAGGAATAAATTTTAAAGCTGCAGAATTAATACAATAGCGCAATCCACCTAATTCACTCGGTCCATCATTAAAAACATGTCCCAAGTGGCTATCACTGTTTTTAGCACGAACTTCAGTTCTTATCATTCCGTGAGATTCATCACGGTAGTTAGCAATTAAATTTTTATCAATGGGTTTTGAAAAAGCAGGTCAACCACAACCAGATTTAAATTTATCAGTTGAAATAAAAAGTGGTTCACCAGATGTTATATCAACATAAATTCCCTTTTCAAAATTGCGGTTATATTCATTAATGTAAGGTGGCTCAGTGTGAGCATTTTGCGTTACATCAAACTGGAGTTTGGTTAAAGTACGTTTTAGCTCAGCTTCACTCTTTTTTTGGTATTTACTCATTTGGAATGTTGTTTATTTAAATATACATCAATAAATGGTTTTCCTACTTTGATCTTAGGATTAATCTCAATTATACCCTCATTTTTCTGTTTTAAATTAAGTTCTTTTTCAGAACATAACATGCCAACTGAATCAAAACCAGCTATTTTAGTTTTTTTGATAACTGTACCGCTAGGTAACACTCCACCTACTTGTACTAAAACTGTTTTCATACCAACTCTAACATTATTAGCTCCACAAACAATATCCAAGCTTTTTGTCAAACCAGTGTTAACTTTACACCTTTTTAGATGGGTGTTTGCAATAGGAATAACTGAAACAACTTCACAAACAACAAAAGGAACTTTATTTGCTAATGAAATAAGATCATAACCTAAAAGCTCACTTATTCTTTTCATTATTTTTAATGAAGGATAGTTTAGACCTTCATTAAAGCGGTGTGATAAAAAAACTTCTTAAAACTTTTTTTGATATTAAAAAAGTTAAAACCAGTAATTCTATTTGCATCATTAACAAAAAAACTTCAGTCTTTGTCATTTTGTTGGCGTAAAGTTGTTTCACTTCTACTTCCTATGATGCCAAACATACAGTTTTTTAATGTTTCTTTACGATAAAAGATAGAAACAAAATCTTTAGATATATCAAACATAAAAGTTAGTTATTCTGTTTGTATTAAAAAGGCATAACTATTGATACCTGTGTGTGTACAAATTACAGGTGAAAGCAAGTGCTCACTTACATCAACTGCATTGTTGAGAAGCGTAACAAATTCTTGCTTTATGAGTTGATTTTTATCAGTTTCAAACATTGTTGTCAACAAAGCCGCTCTTCTGAAATTAACTTCTTTTTTTAATAGCTTTTTTTCTAAAAATTGAACTGCTAACTTATGAGCACTGCTAGCAGATTGTGCTTTGTTAAAAAACTGCAATTTGCCTGTTTCACCAAGGAAACTAATTAAGAGATGAAATTTAAAGCTTTTGATAATAAATGACTTTAAATTAGATAATCTTCCTCCAGCTACTAACGGTTTTGTATCAGTTACAAATAAAATTGCACCGCACTTATTACGGAAGTTATAAACAAATTCATCTAAGCCTGCTCTTGAATACTGGTTGTTTTTAAGTCAAACCTTAATATCACTAATCAATCAAGCTATTAAAACTTCAACTATTCTGCTATCTAAAACATGAAACTTGTCTTCAAATTCCTTTTCAACAATCTTTCAATTTAAATAGGAGGTTGAGATCTCTTTATCAATAGGGATTCCTATGATAAGATCATAATCATTAATTATGCTCTTAACTTTGTCACGAACCTCTTCTTCAGAAGTTTGCGATGTTGAAAACTTAACACCTTTAGGGGCGTTAATCATCTTATCAGTTAAGGTTTTAAGATCAATATTAAAACCTGATTTGAATGTTTCTTGGTGTTCAGCAATTGTTTCAATAATATAAAGCGATAAAACATAGAGATGACTATTTTTATCTTCCTTTAGATTACTGACAGAATCAACTAAAAAAGCGATCCGCACTATTATTCTAGAACTTCTGTTACAGTGCCTGCCCCTACAGTTCTGCCACCTTCACGAATTGAGAACTTACTACCTTTTTCACAAGCAATAGGAGCAATTAACTCAACAGTAATAGAAGCATTATCACCAGGTAGAACCATTTCAGTATTTTCAGCTAAAGCAATAGAACCAGTTACATCAGTGGTACGGAAATAGAATTGAGGACGGTAACCGTTTAAAAAACCAGTGTGTCTGCCACCTTCTTCTTTCTTTAAAGCATAGATCTCAGCTTTAAATTTCTTGTGCGGTTTAATAGAGCCTGGTTTTGCTAAAACTTGACCTCTTTCAACTTCTTTACGTTCAACACCACGTAATAATACCCCAGCATTGTCACCAGCCATTGCTGAATCAAGTTCCTTTTTGAACATTTCAATTCCAGTAACAACTGCTTTTCTAATTGGTTTTAAACCAACAATTTCAACTTCTTGACCTACTTTGAGTTCACCTCTTTCAACTCTTCCTGTAACAACTGTACCTCTACCAGTAATGGTCATCGTATCTTCAATTGCTAATAAGAAAGGTTTATCTACTTCACGTGTAGGAGTTGGAATCCATTCATCAACTGCTTTAATCAAATCATGGATCTTAGCCTCCCACTTTGGATCACCTTCCAATGCTTTTAAAGCTGAGCCATAAATAATAGGAGTGTTCTTACCATCAAAACCATAGGAAGTTAACAGATCACGTACTTCTTCAGCAACAAGTTCTTGTACCTCTTCATCACTAGCAATATCACACTTGTTTAGAAAAACTACCATTTTAGGAACCCCTACTTGGCGGGCAAGTAAGATGTGCTCGCGGGTTTGGGGCATCACACTATCAGTTGCTGAAACAACTAGAATAGCTCCATCCATTTGTGCAGCACCTGTGATCATGTTTTTAATGTAGTCAGCATGTCCAGGACAGTCAACATGGGCATAGTGACGTTTGTCAGAAGAATATTCTACGTGTGCAGAGTTAATTGTAATTCCCCTTGCTTTTTCTTCAGGGGCTTTATCAATTTCATCATAACGCGTTGCAGCTGATTTTCCTTCCTTTGCTAAAACTGTACAGATAGCAGCTGTTAAAGTGGTTTTACCATGGTCAATGTGACCAATGGTACCAACATTGACATGTGGTTTGGAACGGTCAAATTTCTCTCTTGCCATTTGTTTAAATTATGTTGTTAATTTTAAATAAGTTTAAGATAAGCTGGAGATAATGCTTATAATCTTCAAGGACTTAAATTTAGTGAAAACAAACAATTGCCAAATTATTGGCTGATGAAAACAACTTTCCTTACCACAAAAGACATTCTTAAGTTTCATTCCCTTATTTTTGGTCACTAGTGCTTTTGTTTTAACTGGAATTGTTGAAAGCCTTTTAACATTTGGAACTATTATTGAACAAATTGATAAATTCACTGATCAGACTAATGTGATGTTATTAATTTATGCAGTTATCTACACTTTTAATCCAAAAAGTTGATTGTTAAAAAACCAACAATTCTTTTTAAGTGCATTAGCTTATATATTATTTACTTTTATTGGCTATAACCTAATTTTGTCAATAGCTGGTATAGCTTATAAATCAACAAATCCATATAAGTTAACAAGTAGTATTTTTCTCCATGTAATTGCACCAATAGCATTCTTCATAGCAAGTTTTATCAAAATAAAACATGAGAAAGATGTCAATATTAACATGTTCTTTAAAAGCCTATTATTATTCATGATCTATCCTTTAATATATGGGCTTTATTTAGTAACTATTCCATATGTAAGGCATTATCTTTTTAATGGTAGGCCATCTACTTATACAATTTATGGCAGCATTACAAATACTAAAGATAATCCTTTTGCTTGATTAGTTGTATTTGCAGTTTTATTTATCTATTTCCCCTTGAGTTACTTAGCTATATATCTATTACAACTTAAGTTAATAAAAAAAGCCATAAAACCGCAATTTAATTTGCCTTTTACATTAAATAAATGAAAACAAAAATAAGAAAAGCAGTTATTCCTGCTGCTGGGTTGGGTGTTAGGTTACTACCAGCAACAAAAGCAATTCCCAAAGAGATGTTACCATTGGTAAATAAACCTACTATCCAATACATAGTAGAGGAAGCAGTTAAAAGTGGCATTGAACAGATTCTTGTCATTGTTTCATCCAAAAAAACAGCTATATTAGATCATTTTGATTATGATCTGATCTTAGAAAATGCCTTAATTCAAAAAAATAAATTGCAGGAGCATAAAGAGATTGAAGATATTGCTAATTTAGCACATATCTTTTTTGTTAGACAAAAAAATCAAGATGGTTTGGGAGATGCAATCTTGTTTGCTGAATCTTTTGTTGGTAATGAAGACTTTGCAGTATTGTTAGGTGATGATGTTGTTTTTAGTAAAGAACCTGCTTTAAAACAATGCTTGGAAGCTTATTATGAAACTAATTGTCAAACAATCGGTGTACAAGAAGTAGATCCTTGTCATGTTGATAAGTATGGAATTATCACCCCTGAAGGTGATTACAAAAATAAAGATCTTATTAAGGTTTTAGCAATGACTGAAAAACCTAAACCAAAAGATGCTAAAAGTAATTTAGCAATCTTAGGGCGATATGTACTCAAACCATCTATTTTCAAAGCACTTAGAAGTGTACCTTATGGAGTTGGTGGTGAGTTGCAGCTAACTGATGGTTTAAATTTTTGTTTGAAAAATGAAAACTTTTATGCAAGAAAGTTTACTGGTACTAGGTTTGATGTTGGCACAAAGAGTGGTTTTATTAAAGCAAATTTATTTACTGCTTTAAACAATAAAGATATTAGTAAAAAAGAAGTTTTAGAACTTTTAAATTTAGTTAAAGCTTAATACCATTTAAAGTTAAACCAAGGAAGTTTTCACTTCTAATCAATCGACTAAAAGGACACATTTCATGGGCTTTTTTGATTAGTTTTTTACCAACTTCTTGGTCATTACTATTAGTAGTTAATTCAACACCAGCTTTAATGTGAAATAGTCCATTTTCTTGATGGAGTTCTACTTTAACACTTACAACTGGTTTTTTTGAAAAACTAAATTGATGTTGTTGCATAACAACAATTACTGCTTGAGAAAAACAACTTGCATAAGCAGATGCAAATAACTGCTCAGGATTATTTTCTGTTTGAACTGATAAATCAGGTTTGGGAAAACTAAGTTTTGTTTGAAAACCATCTAAAGTTTTAACACTACCTTCTCTCCCAGTTTCAGTTTGTGCAACAGTTTTATAAATCAATGCCATGTTAATTAATAATGATAATATTTTTAGTAAAAATGTTAAATAACATATTGCAATTTCTCAAAGAAAGAGAACTTTATTCACAAGCTAATTTTGAAACAGAACTAGATAACCATTTAAAAGAGAAAAAAATTAACTTTTATGTTGGTTTTGATCCAACTGCTAATTCTTTACATATTGGCAATTATGTTTTAATTCACATTGCAAAATTATTAAAAGACATGGGGCATACTCCACACATAGTTCTAGGGAGTGCAACTGCTTTAATTGGTGATCCTACTGGCAGAATTGAATTAAGGAAAATTTTAGAAGAAAAAGAAATTGTAAAAAACACCAAAACAATTAAACAACAAATCAAACAGTTTTTAGGTGATGTAATTATTCATGAAAACAAAGTTTGATTAGAAAAACTTAATTACATTGAAGTTATCCGTGAATTGGGTGCTTTTTTTTCAGTTAACAAGATGTTAAGCACAGATGCATTTAGTGTTAGGTGAGAAAAAGGGCTAACTCTAATGGAATTAAACTATATGATCTTACAAGCATATGACTTTTATTATCTACATAAAAACCATAATGTCACTTTACAAATAGGTGGAAGTGATCAGTGGGCTAATATTTTGGCTGGTGCTAACTTAATTAAAAGAAAAATAATGCTAATGTTTTTGGATTAACTGCTAATTTATTAGTTAAAGCTAACGGAGAAAAAATGGGTAAAACTAGTAGCGGAGCATTATGACTTGATGAAAATAAAACTAGTGTTTTTGATTTTTATCAATACTGGATTAACCTTGATGATCAAAGCTTAAAAAAGACTTTTTTAATGCTAACAATGCTTGATAAAAAAGTAATAGATGAATTGTGTAATTTAAAAGGCCCAAAAATTAAACAAACCAAGCAAATGCTAGCCTTTTTAATTACTGAATTAGTCCATGGCACTAAAAAAGCAAAAGAAGCACAACAACGATCTGAACTAATATTTAGTAATCAACCAGATCTTGATATTAAGTTAGTAAAAACAAGCACTAATCTGATTGATTATTTAGTTGAAACTAAATTTATTAAAAGTAAATCAGAAGCAAGAAGATTAATTAGTCAAAAAGGTTTGACAATTAACAATAAACACGTTTTGGACTTAAACCAAATAATTGAATGAAAAGAAGAGTTACAAATTATTAGAAAAGGTAAAAAAGTTTTTTAACAATTAAAGCTGTTAATTCTTAGCTAATTTATCTTTAACTTCTTTTTCTATTTCAGTTTCAACACTTTTTTGACTAGAGCTTTTACTTTCTGGTTTTTTGATTAATTTGTTATGAAGTAAATCAACAACTTTCTGCATTGAATTTAATGTTTTGAAATCATAGCTTGCTTGGAGTTTAATGTTTGTAACTAGATCATCAAATGCTTCATTCACTTTTAGTTTAGTATCCATAAAGGTTGTAGGCACACTCATAAACAAGTAAGTATGAACTGTGTCATTATAGACATTAAATTCGGCATATACATTCAAAGGATTAAAAAGCACTTTATTTAATACCTCTGTATCTAAATTCTGAAAAGAAAAATTTGAATCTTTTTCAACAAAAAGTGAGATGTTTTCTTGCTTTTTGTTTTCAGTAAGTGCTTCAAACTGAGCCACATTCTTATTTAAAGAATAGTTAGCATCAGTTAAAAAGAAATGATTTTCACTATTAATTGCTGGTGTAATAGCAATCAAGCAAGCTACTTTTTCTGTTTTATTACTGCTTCTTTTAATTGCATAAATATGACCAAAAACAAATTCACTACCATCTTTATAAGTAACTTTAAATTGTCTTGGAAGTGGTTCAAAATTGTAAAGAGCAGTTGGATAAAAACTTGGTAATATATCGCTGCTTATAGGACCTTGTTCAAAAAATTCAAATGATTCTATTGCCTTGAAATTACCCATCTCTTTTTGTCATAAATTAGTTCTGTTTTCTTTGTTAACTTTTTTTAATAGCTTGTTTCTTTTAAAAAGAGCAGCAAAATGGATAACAAATGCTAAAAACAAAACAACACTACCTAAAACAATTAAAAAAAGTGATGCTATATTTGGTATTTCATTACGTGTATAACTATAAATACCAAATGAGATAGCAATAACACTAAAAAGACCACAAAGTCCAATTACTCTTAGAAAAGCATTGAGTTTATTTGGTGTCAAAAAGAACTTGTTAAATTTAGTTAGATCTGACATATTTGTATAAAAGATTGTATGAAAATATAAACCAAAGCAAGCTCAACAGTTGTTTTATTACAAGATCTTTTTCCTAAAAACTGCAAGTTGCCAAACTTAGCACGCATCTTTATTAAACGAAGATAGAGCTCATCAAATTTTATATCAAGTATTTTGTATCTTTTGTTTGTTTTGGAAATAAAAGTGCAAAAGTACTTTAGAAAATAAAAAATCAGATCCCAAATTCGTTTTTGTCCTAAACTAATAGTTTTGTTTTTTAGTAATACTTCAGTTTGCTTTAAAACATTTTTAAAAGTAATAACAAGATCATCATTACTGTCATTTAAATAAGTTTGTATGTTTTCAATTCAAATCAAAATGTAGTGATATTCACTGTCAAACAATTGGGGATTATTTTTAATTACTGTAAAAAAATCAGTAATTGTTTTACTTTGATCATTTAGTACTTTAAAACAATTCCAAGCTTCATTACCATAACCAACTTCTATTGCATTTAATTCTTGCTTAAATGATAGTAAGTTAACCAAGAAGTTATCAATAAAATCAACAACACTAACTGTTTGTTTCACTTTCTGGTTTTACTTCAGATTTATTTAGTTTTTGCTTTGCTTGTTGTTCTTGCTTTTGCAATAAGATTTCTGGTGGTAGTTTAGTGTTTTTATGGATGAAGTCAATATCACTTTTCAAAATAGTTTCAGCAATTAAAAGTGCTTCTACAAGCAATTCTAGTTCCTTACGGTTACTCTTAATGATTGTTTTTGCTTTCTTATACTGTTCTTCAATAATGAAATTAATCTCATTGTCAATATCTTTAGCAGTTTGTTCTGAATAAAGTTTTACATTAGAAGGGAGTGTACCTTGACTTGGTACATATTGCACTTGACCTAATTTAGACATCCCAAGCTGGGTTACCATTGCTCTTGCAATATTAGTTGCTTTATAAAAATCGCTAGAAGCGCCAGTAGTAATTTCTAAATTACCATAGATTTCCTCTTCAGCAGCTCTACCGCCCATAGCAGTTGCTATCATTGCAAGTAAATCAGATTTTCTTTTTAGGTTAAGATCACCACTCTTAGGTGTTGAAAGTGTGTAACCCCCTGCTTGACCACGAGGAATAATGGTAATCTTTTGTACTTCATCATTACTGTGGACATGTAAACCAACCAAGGCATGACCAGCCTCATGATAAGCAACTAGTTTTCTATCTTCATCACTAATTACACGTGACTTTTTAGCAGGACCAGCTATTACTCTATCAATTGCTTCATCAATGTCATTAATGTTAATTGTGGTACGGTTGTCTCTAACTGCTAACAATGTAGCTTCATTGATAACATTTTCTAATTGAGCACCTGAAAACCCAGGAGTTCTCTTAGCAACATCTAAAAGACTTATCTTAGAAGAGAGATTTTTATTTTCAGCATGAACTTTTAAAATCCCTTCCCTTTCTTTAATATCAGGGAGATTGATTTGAATATGTCTATCAAATCTTCCAGGTCTTAATAATGCATCATCTAATACATCTAACCTATTTGTAGCTGCCATTACAACAACACCTGTTCTGCTTGTAAATCCATCCATTTCAGCTAACAATTGGTTTAAGGTTTGCTCAACAACAGAATAAGAAGAGAGTTCAACTCTACCCCGTTTAGAACCAACTGAATCAATTTCATCAATAAAAATAATACAAGGAGCAGCCTTTTTAGCTTTATTGAAAAGATCTCTAACCCGTTTAGCACCAACACCAACAAGCATATCTTCAAATCCAGAACCCGTTGATTGAAAGAAAGGAACACCAGCTTCACCAGCTACTGCTTTAGCTAATAATGTTTTACCTGTCCCAGGTGGACCATATAAAATTACCCCACGTGGGGATCTTGCTCCCATCTGGGCATATTTCAATGGATTTTTTAAATAATCAACTATCTCAAGCAACTCATGCTTTTCCTCTTGAAGTCCAGCAATATTGGTAAATTTCACAGTTGACTTAGCCAACTTAGCTTGGGTTTTGCCAATAGAAAAAATATTATCTTCTTCTCTGCCCCCTGCAGATATACCCCTAGCACTTCTTCAAAAGAGTAAAAAGAAAACTACAAAGATAATAATTGGTAGCAAGCCAAACAAACCATTTAATACATCTCTAGCTCTAGTATCAGGAGCAATGAAAGTACCAAGGGTTTCAAAACCTGCAATACTCTTTGTGTTATTACTAGAACCACCATTCATCATGGTTCCATTACTATTAACGGTAATCTGACCATTACTTTGGTTAATTGATAAGTTAGCAATATTAAAGATAACATTGCCATTACTATTAACAGTTTTGTGAGCAGTAACAGTTAATGGACTATTTAAACCATCAAAGGTAATGGAAACTTGGAGAATGGTATCAGTAACATAAGTTGAACCATTTATTTGTTTAAATGTCAGTTCATTACTAAAACCGCTTACTTTTGCTGTTAAACTACTACCATTGTTTAATTTTCAGCTTTCAACTACTGCCGTTGCAGCTCTTGGACTGAAAATATAAGCTAAAACACCAATAACAACAGCTAAAATGATGACTCATCAAAAGACTTTTCAAGCAGTTTTACGTGAAAAATTATTTTTTTCAGTAGTTGTTTGTTCTACTAAACCCTTATTTCTTTTTTTCATCTATGTTAGTTTTTGACCGATTTGGGTTTTTTTGGTTTGAACTACATTAACTGTAATTATACTGCCAATGGTAATGAAACTAATAAGTGTTAATAAAGTGACGAAAATTATCTTTTTATGTTTACTTAAAAGTGCAATAAAAGCCTTGAACCAACGCATAACATTTTAATATCTGTTTTAGGGATTGTCTGGTTCAAACACACCAACATAAGGTAGGTTACGATAAAAACCATCATAGTCAAGACCAAAGCCAACCAAAAAATTATCTTTTACTTTAAAACAAGAAAAATCAATATTAATATCAAAGGCTTTGGGTTTAATCTTTTCAATTAAGCTAATTAAAGTTATTGATTTAGCATGCCTTGTTTTTAGAAGATCAATAACTAATTTAATAGATCTACCACTATCAACAATATCTTCTATTAAAAGGATGTCTTTATCTTTAGGGTCATGGGACATATCAAGCACAATCTTAGGTGGTTGTTTTTGTACATGTGAACCATGATAAGAAGCAACTGCCATAAAATCTAGTTGGAGGTCAAAACTAAATTTACTTATCACTTTGCCAAGGAAAGGGATGCAACCTTTTAGAATGCCAAGAACAATTACCTTTTTATTATTAAATTTAGCATTGCACCAATTAACTGCTTTTTGACAGCCTTCTTCTATCTGTTGTTCATTAATAACAATAGATTTAATACCCATCTTATTTAGTTTTTTGACGTTCTACTAAAACAACAGCTAAGCAATTAATTTCATTATTGTCTAAAGGTTTTCTAACTTTGAGATTAATTGTTAATTCTGAAATTCTTAAAGCACTACAAATAAAAGCAAAGATAGCGTGTTTATAATCATCAACCTTTTGCTCTTGAGGAATCTCTAAGGAGATATCAATATTATTGATAATTCAGTTTTTTCTGATGAAATTGTAAGTTCTTGCTAAGACTCTTGGTGCTTCTTTTTTTGCAGTACCTTTAAATTGGACTGTTGATTTTTCTTTATTGAAAACAATATGACCATCACCTAATGCAGTTGCACCAAATAAAGCATCAGCAACTGCTAATTGGATAACATCACTAACTTCATCACTGTTTTCAAGATCATAAATATATTTAGAATTCTCAAAATCAACACCGCCTAACCAAAATTTATTTTTACTTTCTTTCCTTAATTTTATGTGGTATTTTTTGCTTCCTAGGCCAACTCTTAACTGCATTGTTACAAATAATTATAATATCGCTCATCTTTAAATAACAGTGTTGTTTTGTAATCCAAAAAAAGTAAATTCAGGCAACAGTTTTTTGACTTCAAATATCCTTATTTAGACAAAGCAAAATTTATTAAAGTGAACCAAAATAAAATTGAAGTATATCTCTAGATAAATAAATATGAAAGGACCTAAAATTGCAATAGTAGGATCAGGTGCTGTTGGTACTAGTTTTCTATATGCTGCAATGACTCGTGCACTTGGTAGTGAATACATGATTATAGATATCAATGAAAAAGCTAAAGTTGGTAATGTATTTGATCTTCAAGATGCTTCCTCATCATGCCCAAATTTTGGCAAAGTAGTTGCTGGAGAATACAGTCAATTAAAAGATTATGATTTTATTTTCATTAGTGCAGGAAGACCTCAAAAACAAGGTGGGGAAACTAGGTTGCAGCTACTTGAAGGCAATGTAGAAATTATGAAAAGCATTGCCAAAGAAATCAAAAAATCTGGTTTTAATGGAGTTACATTAATCGCTTCAAACCCAGTTGATATCATGTCATATACATATCTTAAAGTTACTGGATTTGAACCTAATAAAGTAATTGGCTCTGGTACTTTACTTGATAGTGCAAGATTAAGATATGCAATTGCAACTAAGTACCAAATGAGTTCAAAGGATGTACAAGCATATGTTATTGGTGAACATGGTGATAGTTCAGTAAGTATTATTTCATCAGCTAAAATTGCAGGACTATCACTAAAACATTTTTCTAAAGCTAGTGATATTGAAAAAGAATTTGGTGAAATTGACCAATTTATCAGACGCAGAGCTTATGAAATTATTGAACGCAAAGGTGCTACTTTCTATGGAATTGGTGAAGCTAGTGCTGATGTAGCTGAACAAATTTTGAAAGATACTAAAGAAGTTAGAGTAGTGGCTCCTTTACTTACTGGTCAGTATGGAGCGAAGGATATGATGTTTGGAACTCCTTGTGTACTTTCAAGAAAAGGTATTGAAAAGATCTTGGAAATAGAACTTTCAAATACTGAAAAAGTTGCGCTTGAAAATTCAATTAAAGTTTTGAAAGACAACATTAAACTAGCAAAGCTTTAGTTTTGATAGAAAACATTAGCTCATCTATAAAAAGAGTTTTCAAAAAATAACTCGTTACTTCTCTTAACAAGCATACTAATTTCACTGAGATTTTTAATCTTGTTTTGAGAAGTTTTTATTTTTAATTCATTTTTGTGCTTATTTCATCCTTCATAAAAACCCTTAAATTGGTTATTGAATTTAGTAATGAAATAACTAGGAGTTTGATATTCTTTCACTTTTAATTCAAAATCAAGATCACGTTTTTCACAATTTTCATAAAACTTTACTTGAAATTTCAGAGTGTTAAGATAACTATCTAGTAAGGTTTTTAATATTGCATCTTTAGTTGTTTCAAATGTATTAACTAAAAATGAGTGAAAAACATAATCATCTAACTTCACAAAACAAGTTAATAAACTTTTTTTAAACAACTGGTTTTTTAAAAGCGGTTTAATTACACTTTCAACATTCTTAAAGTTATGAAAATTAAAGTTGTCTTCTTCAATTAAAGCTTTAATCCGTTGAAAAACAAATCATAAGTTAAGTTCTGTAGCAATACTTCTACCATCACTGTAGATTGATTGATACATGTGGTGTCTTGTTATCAAGAAGTTTTCAATGAAAGGTAAGCATTTTTCTTGAAAAACAATTTCATAAGTGCCCTGATTATCTACACATTCCATTCCATTGATAATTGATTGATAATCAACTAATGAATGACTGGTACCTGTAAAATAAGCATCACGTAATAAATAATCCATTCTGTCTGTGTCAAGATCTGAAGATATTAGCTGTCTCATCCACCAGTTAATAGGTTGAATATTTTGATTTTCATCAATTAAAGCGCCAATCAGATTTGGATCTATCTTATTAGCTTTTAAGATAGAGACAATAGGTTCACTATTAACTAACATTGAAGTAACCTTTTCATGAATAAAAAGTTGTTTTTTAAAATCAGGATTTTTAGCAAAATAGATTTCAAAAGCATGAGAATGAGGTCCATGACCAAGATCATGCAACAAACCAGCTACTAAAACAGTTTGTTTTTTAATTTGATCAATATTTAAAAATGCAGAACTATTTAAGATCCTTCTTATCAGTTCATAAACTCCTAAAGAATGGGCGTACCTAGTGTGAACTCCACTAGGATAAAAATGGAAGTTAATTCCTAATTGTTTGATGTTTCTTAACCTTTGAAAAGCTTTTGTATTGACCAATTCATACATCCACTTTGTGTTTTCATCAAAAATAATTTCTCCTAAAATAGGATCTTTAAAAAAGGTTTGTTGCATTAATAAATTGCTTAATTAAGTTTATTGCACTTTCTTTATCAAAAATTTCAATAATATGTGCTAAATCAGGTCCATGCTCCTTATTTGTAAAAATTAACCTTATTGGCATAAAAAGTTGTTTACCTTTTAAGTTAAACACTGCTCCTACTTTGTTAATAGTTGTTTTAATTTGCTCAGCTTTTCATTCTTCCAACCCTTGTAAAGATTTGGCAAGCTGTTCTAAAAAAAGTTTGATGTTTTTAAACAAAATATGACTTTTCTTAGCTAAGTTTTCAACACCTATTTTAGTGGCAAAAGATTCTCTTATCAATTCGTTTATTTGAACACCATGGGTTATTTGATTTTTAAAAAGTAAACTTATTTCCCTGTTTTTATCTTTTAAATAATCAACCTTAACATCCAAGTAATTATCAATGAAATTGAAATAAGCATTATCAGTTAATTGTTTAATGTAATTAGCATTGATTCATTGCAGCTTTTTAATATCAAAAAAAGCAGGAGCACTAACAACTCTACTTAAACTAAAGTTTTCAATTAACTGTTTCAAATTAAAAAACTCCTGGTTGTACTGTGGATGTCAACCTAAGAGTGCTAAGAAATTTAATAATGCTTCAGGTAGATAGCCTTGTTGTTTAAACTGCTCAATAAACTGAGTAGTTTTCTCATCACGTTTAGAAAGTTTTTTGCCACTTTCATCAACAATAACTGAAAGATGACCAAAGCGGGGAATTCTTTTAAAACCTAATGCTTGATATATAGCAAGTTGGTATGCAGTGTTAGAGATGTGCTCAGCTCCCCTTAAAACATCAGTAATTTCCATATCATAATCATCAATAACAACTGCAAAGTTATAAGTAGCAACACCATTAGCTTTAAGAATAACTATATCTGTTAACGCACTGCCGGGAATAGTAATTTGTCCCCTAACCAGATCATTTCAACTATATTCAGCTTCATTGTTTATTTTTAAGCGGATAGTAAAAGGATCATTTTTTTCTAAGTGATTAGTAATTTTCTTGGAATGTAAATTACGACAATGACCTAAGTATTTAGGGGTTTTGTGGTTATTAATGGCTTGTTTTCTATCTGACTCTAATTTTTCTTTGCTACAGAAGCAACGATATGCCAGATTTTTTTCAATTAAATCAAATGCTAGTTTTTTATAAACTGCTAGTTTTTGTGATTGCAGATATGGACCATAATTGCCAGGGTTATAAACCGATTCATCTGCAATGACTCCAAGCCAACGCAAGTTATCAAATTGTGAATTAATTCCTTCTTTTATGTTTCTTTCAGTATCAGTATCTTCTATCCTGATAATAAACTCACCATTAAAGTGCTTGGCTAGTAAAAAGTTAAAGATTGCTGTTCTTGTACCACCAACATGCAGATATCCTGTTGGGGATGGTGCATAACGTGTTCTAATTTTTTCCATTACAAATGTTTTGGTAAAGATTAAAGAGTTGACAAGGACTCAACTCTTGCGCTCTAATGCTAGTCTTTAAATTTTGCTTTTGAATAATGTTAAGTAAATAATCAACTGCAAAAAAATGCTTTAAGTTATTAATTAACATCTTCCTTCTTTGATTGAAACATTGCTTTAAAAACAGACCAAACTTAAAGTCATAACTAACCGATTTATTTTTTTCTAATAATATTAAGGTTGAATCCACCTTAGGTTTAGGTTTAAAAGCATGCCTATCAATTTTAAAAACTGTTGTAATAGTCAAATAGTATTGACAAAAAGCACCAAAGGCACTATAATCACTAGAATTAACCTTTGCCAGAAGCCGATTAGCAAACTCTTTTTGTGTCATTAAAACAAAGCTTCGAAGCTTTGATTCTAAAAACTTATTGATTATTGGAGATGTAATGCTATATGGGATATTACCACACAATAATGGACTTAAATTTTCAAAAAAACTATTAAAGTCCTTTTTGAGAATATCGCCTTTAACTAGTTGGTCTTCAGTTAATATCTTTTCAACTAGAAGATATTCAATTAAGCGTTTATCAATTTCTATCCCCTTGTAAGGTATTTTGAGTTTTAACAAATAATTTGTTAACGCTCCTTTACCAACACCTATTTCAACAATTGCTTGTGGATTTAAATTTTTAACAAAAGCAAAAATTCTTTTAATGACGCTTAAATTAACCGTAAAATTTTGACCTAATTTACGTGAAGGAAAAAAACTATTCACGCTCTAAAATCAAATCTAATTTGGAATAATTTTGCTTTCTTTTTTCCCTTCTTTTGACAATGAAAACATGGGTTAAATAAGATTGTAATAAAGTAAACAAAGCATTTAAAAACCAATAAACACCAACTCCAGCAGCACTAAATGCAGTAATAGCAGCAAACACAAAGTAAAAAATTAATTGCATCTTTTTAGTTTTGTTGAGTTGTTCAATACTTTTTTGAGAATGGGCTTTCGCATTCTCATTACGCTTACTTGCCCACACTTGAGGAAGTTTTTGAGAGAGAAATTGGACTGGTAAAACAATCACTAAAAAGATGATGAAAGGTCAACCAGTTGTAGTGAAATTAGAGAAAATTTCTGTTAAAGGTACTTTTGAAAGATCCCAAAAGTTAAATAAGATGATTGCTTTAATTGGTCTTAAAGTAGTAACAATCCTATAAATAATTAAAAAGATAGGTAAAGTTACAAAAACTTGAACAAAAGCAGCAGATGATTTAATGTTGTGCTTTTTATAAAGTGACATTATCTCTAACTGTCTGTTTCTTTTACTTTGTAAATCTATTGCCCCTTTATATTTAGCGTTAATTTCTGCTAATTTACCTTGCACTTCATTCATCTTTTCAAGTGCTAAAGTAGAGTTTAAGGTAATCACAATAGTTATCAAGCGAACTAATAGTAAAAGTACTATTAATGAAAGAATCATGTTAAACCCAAGTTCCACCCCGCTACCTAGTGGAACTCTGGTTGCATACATAATTGGTAAAACTATTTGCGCTGCAGGCCAGACAAATCATCCATAAAAAGGGCCATATGCTAAGGTGTAATCACTAAAGGTGAAATAAGGGCCAAAGTTATTTGAAATCAAATCATACCTATAATCACCAGTAGTACCAAATTTATAACCTATCTCAAGTCCTGAACCTAAGACTTGATTAGTACCTGTTCAGGGTTGTGCTAAGGTTTGAGTACAGCCCCAAAGCCCAACAATAGTTAAAAAAATAAAGATAAAAACCTTAATAACCTTTATGAAAATAGCTGAAAACTTTTTGAAGTTTTTTCAGTTGTTTTTTTCATTAACAACTGCTGCTGATCAAAAAGGATTAAAGGTGGTTTTTATCTCTTTATTTGTTTGCGCTAGTTTGATAGGCATCTACTTCTTTTATCCGCTTTAATAGTTGCAAAAAAAGTTTTTGTTTTTCTTTAAATGTTAATTCAATAAAGCCTTTGTTGACAATAACTAAAATATCTCAAGGTTCTAAATTATTACTAATTTGTTGAAAGATACTACGGATCTGACGCTTAATTAGGTTACGTTGTACTGCTAGCTTATATTTAGTTTTTGCAATTGATATTGCAACCCTTCAAGTAGAATGATTATTCTTAATAAAATAAGCGTTAATAAAGGTACCAAAGAACCTTGTTTTACTTTGAAGAATGGTTGTAAAAACCTTGCGTTCTCTTAAGCTGTGACTCTTTTTAACGCTCACTGGAAACCGTGAGTTGAGCACGATTTTTAAAACGTCTTTGCCTTAAAACTTTACGTCCTTGTGCAGTTGCCATCCTAGCCATAAAACCATGGGTTTTAGCACGCTTTAATTTGCTTGGTTGGTATGTTCGTTTCATGAAACAATTAATCAGGTGATTATAACTAAACTAAGGGTCTGACTAATTGGTTAGATACTATTTTACCATCTGAAATTTTAATGATTCTATTAGCTATCCTTGTTAGGCTTTCATCATGGGTAACCATTAAGATGGTTTGCTTATATGTTTTGTTAATTGTTTGTAATAAATTAATGATATTTTCCGCAGAATTACTATCAAGTGCACCAGTAGGTTCATCACAAAGTAATAGTTTAGGTTCTTTAATAATAGCACGGGCGATGGCCACTCTTTGTTTTTGCCCACCTGACATCTCATAAGGAAATTTATACAAGATTTCTTTAATATCTAATTTTTCAGCCAACTCTTCTATATCAAGTCGTCTTTGATATTTGGGAATTAAGTTTTGTGAAATGGCAATATTATCATAAGCACTTAACAATTCAATCAGATTATAACGCTGATAGACATAACCAAGGTTATTCTTTCGATAAGTTAAAAGTTGTTCACTAGTACATTTTTCTAATGGACAGTTTGCTACGATACAACTCCCTAAAGAGATAGAATCATAACCACCTATCAGGTTTAAAAGGGTTGTTTTACCTGAACCTGAAGAACCTAAGATGATTACTATTTCACCATAAGCTATCTGTAAAGAAACATCTGAAAGGATAGCAATCTCTTGCTTTGAATCATCATTATGAACATTTTTTCACATCTTATTGATGGTAATAACAATTCCCTTAGCTTGTTGAGCAAAAGGAGGGATATATAAGGTATTGGCTTTTTGCTTTTTATTAGCTTTCTTTTTGAGCTTTAGCTCAGCTTTTTTAATGTTATTTAAAACTTTTTTATTCTTCTTAGGAGGTTTAGATTTACTCTTTTTGAAATAATCATCGAAATTGGCACTAAAAGAAGGACTTTTAACGTTATCTAAAATATCAATACATTCATTTCAAACAGTTTTATTGCCTTTTTTCATTAACTAAAACCCTTCCTTCAGTTCAGCTGAGCGTAACTTAATTCAAAAGTAAGAACCAATTACAAAGATAGTAACACCAAATAATACTATCCCTACCATGTATGGCAAGAACTCAAATACATTAGTTAAAAAGACTTGTGCAACATTGAAAACAACTGCTTGGATACCAATTAAAACACCAGCTAAAACTCCAATAGAAATTAGTAATGCTAAAACAAATGCAGGGATAAAGAAGGTAAGTAAACTAATTAAATTTTGTACATCTCTATAGCCAATAGCCTTAAGTGAAATAAAGATTCTTTTCATCTCTTCTAACATTGAGATACCAAGTACAACACAAGTAATGAAAATGAAAGGAATAATAGTTCCTAAAAGAAACGCATCTACTTGATTCACAGTGTTAAACAAAACTTCAGCATTATTTCTAATGATCTGAGCACTAGTTCCAGCTGCATCAATATTGAGTACAGGAGCAATAATGTTATTACCAAAAATACTACTTGCATTTTGGGAAAGTAAATTAACACTATTTCATTCTGTTTTGTATTTATTAACTAGTTCTTTACGAAGCGATTCATAATTCATTGAAGTAGTATTAATAATCTGTTTTTCTTGTGCTAACTTATTAATAATTTCAGGATCAGTAGGTAGTATGGATGTAATAACTTTATCAAGTTTATTCCTTACTAAATTTTGGAAATTATTACCAAAGTCAGTAAAATTACCCCAAACACCAATTATCGAGTTTAATACTAGTGCCTTATTTAATAAAGAAGGTTTTAATTCTTTGGAAAATACACCATTAAAAGGAACATAACCGTTTGGCTTTACATAATCAATTTCATATTCCTCATTACTTTGAGATTGGCCATTTGTCTTTTTAATAACATCTCTAATAATACCTCTGCCCTGATTACCACTTAGTTTTAATAAGTTATTAGCAATGTGTTGGTTAATATAAAGTTCTTGTCCAAAAGCATCAGTAGAAACACCAACAACTTTAAATGCTACTGTTGGATTATTTAAGGTTGCTTTTAGAAATTTTTCAGAGTACCTTGTGGCAGTATTTTTCGGTTTTATATAAACATAATCACCAATATTAATGTTGTTAGCATATTGAAAACCGGCATTAACAATTACATTCATTTCCTGATCAGAACTAATTAAATTTCTTAAAGAGTTACCACTACCATCTATCAGATTAACAAAACTTGATTCAGGGTTAATCCCTGTTAAAGTGTATGGTGAATTCTCATCAAGATTATCTTCTTGAAAACCAAATGGGCCTTCAACATAAGTGTAGGTTTCTTCTATGGCAGGGTCAACAGGAACAATCCCAAAATTTAACTTAGAATCTTGTTTTGCAAGTTCAAAATCCCCATAAACTTTCAACATAAAACTAAGAAAGCTATCATTAAATTTAACCCCACCAGGATTAAATACATCAATTGTAGTTAGTACCCTACTAGCATCTAACTGAATTTGATTAGTTCTAGGGTTTCTAATTAAAAACCCATTAGCATTTAAGTTATTGAAAAAGTCATCAGTTAATACTGAAATTAAAAACTGATCACTAGATGCTGAAAGTAACTGAGTAATCTGTTTAGGAATTGATTTTTCAACAATTTCCCAAGGGTTAATGTTAGAACCGCCAACCCTAATAGGAAAGTCAATAATCCATTTTGATAGAGAGGCATGCCTAAAGAAATTCTCATCAGTGCTTAACTGTTGAGCACCACCATAACTAGGCAAAAGTAAGTTGCCCAATTCAAGCTCAGTTCCATCTGCTGCTTTTATTGGTTGATCCTGGCGGTTTTTAAGTTTTAAATTACTTGGTTTATAAGGGTGCTCATTTTGAGAATTTGCTTGAACACTTTCGTTATACAAGGTTTTAATACCATTATTATTATCAGTAACTCCAAAGTGTGAATAGGGTTGAATTGCATACCATCCAGATTGCTCTGTTGGTGTTTGTAAATTCAACTTATAGTTAAAGTTTCTATTTAAGGAAGTGCTTGTTTGCGCTGCACTAAATTTTTGGGGAATAGTACCTGCAATCCCAATCAATAAAAGTGCAACTGAACTCAATCCTACATAGGTAAATAAACGTGAAAAACGTGAAACGATTAATGAAACCCTAAACTTGGTCAAGGGTGACATTGTATGACTTTTATGTTTTAAAAGATGAAGAAGAACTGAAAACTTGGTCTCATTGCCTTGATCAATTAAGACATTAACAGGTTTACTAAACAACTGTTTAAAGGCAATTCAACTAATAAATTCAAAAACAAAAAAGGTTATAAAGAAAGAACCAAAAAACGATAACCAGTTAAAGCTATTTTCAGGTAAAGCAATAAACCAATACCTATTTATGATTCCCTTTACTTGTCCTTCTAGAAGAAAGGCAAATAAAACTCCTAAAAAACTAGAAACAATTGCAACAATACCAGCAAACACACTCATTCCAACAATAAATTTAGTTGTTGAAAATCCCCCTGCTCTGATAATAGAAAACTCAGTTTGGTTCTTTTTAATAAATGACTTAATTAAAAGAATAATTAAATAAAGAGCAAGAATAATCAAAAATATAGCAATAACAGTACTTACTATTGCCAAATAGCTTTGCACTAAATCAGGAAAATAGTTTCTTGCTGTTGTCAGATAATTAAACTTAGAAATATCTTTAACATTAAATGCTAAATCACTTCTAGGAACATCTAGATATCCTTCTAATCTATTAGCAATAAACTGCACTGGGTCTTGGTCAGTGTGCTGTTTTAAATCTGTTGACTTAAAAGCATAATAATTTTCTTGGTTAGCTGCTGGTACAGCAAACAGAATACTTCTGTAAGCTTGATCATTAACAAAGATTAACGCTTCATCTTGGGTATTAGGCAACGGAGATTGTAAAGATAACACTGGGTAACCAGTTTCTACTGATTCACCTATACCTAAAATTGCAAATGTTAGTCCGTTAATACTAAATTTATAACGATCAGGTAGATCATTTAACCAGTTAGGGAAATTGTTTGGTGTTTGCAATGCAGTTTTTCAAGCATATTCCCCTTCCTGGGGAAGAATTTCTTTATTGTGTCTAAACAACCATTGTTGAGAAACAACCGCTCCGTGTGCTTCAGGGGTTTGCACAACTAAATTTTGTTGATCAGTAGGTAATATTAATGGATTGACCCCATTTTTAACAGTAATAGGGATCTCAAGCATTTGGCGATTTGTCTCTATTAATTCATTAGGATCTTGAAAAGCAGGAGTAAAATAACTTAAAAGTGCAAGATAATTATTAGCTGCTTTTCGTTTATCTTCTTCCTTAAGATTATTAAATCCATTGGCATCTAAATTAGTTCATTGTTTTAAAGCATCTAAAGCAGTAGTTGGGATATTAGTAGCTTCTGGTAATTGGGCTTGAGCAATAATATTTGCTAACTTTGGATAAATGGGAAATTTAGTAGATTTTGGACTTAAAGCTTGGGTATTTTTTAAACTAGCAGTCATTCCACCACTACTGGTGCTTGGTTTAAATTCATAATCAATTTTTTGTAAAAATCCATTATTAGCATCTTGACCACCATAAATTGTGTCTAAAGGGTTTGATTCATTTTGCTCAGGTTTAAATAACCAAGATTTAAGATCAACGCCATCAATTAAAGAAAGATCGTTGTATCTCTGTTCATCTTTGTTGGAAACTAAAAAACTTGCTGACTCTGCTGAACTAACATTGTTAGCTATTGCGATATTTTGCTTTGAATAATCAATGTTATTGAAAGCAGTGGCTTTTAAAAAATGATCACGGTACTTTTCTAAAAGATCAACTGTTTTTTTAACAAGATCCTCTTGAAAGATACTACGTCATTGATCAAGTACTGCTGCATAAACTGCATCAAGGGTTTTTTCAGGAGTGTTATTGGGCTGGTGAATTTTATTGTATGCAGTTTGAACATTTGAAAAGTTAGGATCAGCTATTCCATTACTTGCTTGACTGTTTTGATTTTGAACTAATTGGAGATTGTTGCTTGAAGGTTGAAAAGAAAGATTATTATTTCTTACCATCATTCCATTCTGAGTTTTAAAGATTGTGTCTTTATCACTATCACTAAAACCTGGTAAGGAATTGAGAATTGTATCTTTCTCAGATTCAACTTTCTTTTTAAGTCAATCTTTATTAATTTTTAGTTCAAATTTCTTAAGTTGCTTAAGTTTATTTTCAAGATCTCAATCTCCAAAAGAGGAATTATTGGCTATAGTTTTAGCATTAGTTTCTAACCACTTTAGCTGTTCATTATCTAGCTTATTTAATTTAGAAATATCTAAATTAAAAGTTAATGTAAAACTTTGATTATCTTTACTTTCTTTTTGGGTTTGAAATTCTTCAACAATTTCTTTAAAAGGATTGACATCTTTCCAAAATTGAGAACCAGGATCGATTTCTAAATCAATTGGGATGTTTGCATAAAGATTGGGAAATCAGTTAGCAGCTTCATTACCACCTGTAAATAGATAGTTAGCAGATATATCAGTATTATCTGCTTTGGTCTCATCACTCTTAAACCAAACTTTATCTTTGTTATTGGTCTTATAAAGTGAATTAACAGTATTGTCTTTTTGTGGATCAGTGTTTGTTTGTTGCTTTTGTGTAATTTTTATTTGTGTTCCTGTGCTTTTTCCTTTATCACCAGGAAAACGCGCAATGCCAGCACGCAGTGAGATAGTTTCTTTAATTAAACCATCAGCAAAATAACCTAGAAGGTTACCACTAGCATCATAAATAAGGTTTTGAGGGTCTGATATTATGTGTCTATTTGAAATGGAATCACTAATATCATTACCATTAAAAGAATCAGTATTATTAAAAGAAACTGCTAACCCTTTATAAATTCTTTTTTCAGCAATATTGGCTTCTCCATATGCTTTTAAAATACCTCTTTTTCTTGCTAAAAAGAGTTTATCTTTTTCATTAACATCGCCTTTGCGGGGAATGGTTTTGTTTTGGTTATCCTCACTACCATTACTTTGTCCTGAATCACTTCCAAAATCATTTTCTTGATAAGGAAAAAGATAGTTAATTTTTTTATCATCTATCTTTATTTTGGAAAAATAACCACTATCATTAGTTAAACTTGCTGTATAAACAGGATTAGCTTCCTTTAAAATAGCAGGAGCTACTATATCAGAAGAGTTAGTTTTAGATACTAATGTAGTGTAGGTACTAACTAAGTTATTACTTAACTGAACAATAGATGTTTCAAGGAAAATAATGCTAAATAAAACAAAGATAATCCCAAATAGTAAAAAGAAGAACTTTTTAAGCGACTTAAATATCTGTTTAAAAAAAGAAAACATCTCAATTAAACTCAGTTAATTTGTTCGATGGTTTTTGGATTCTGATTTAAATAATCAACGATTATCTTGCCATCATGGATTTTAATTACCCGTTTAGCTAATTCCACTATCTTTTCGTTGTGGGTAACTATTACTATAGTAGTACCTTTGTCACGATTATATTCCACAAAAAATTGCAATATTTTTTTGGAAATATCAATATTAACTGCTCCAGTAGGTTCATCACCAAACAAAATTTTAGGTTCTTTAATTAAAGCCCGAGCAATAGCAACACGTTGTTGTTGTCCACCAGAGAGTTTATGAACTTTCTTATGTCTATGTTCTTTTAATTCCAAGCGTTCTAATAATTCTTCAAGATTATTATTAAACCTTTTTTTTAATGGTAGAGCAAGCTTAATATTATCATCAACGTCAAGATCACGCAATAAACCATATTGTTGAAAAATATAACCAACGTTTTTATTTCTTAACGCGGTTAGTTTTGCATCACTACAACATATGGTGTTAGTTCCACAAACAAAACTATCGCCGCTAGTTGGTCGATCTAATGCGGAAATTAAAGAAAGTAAGCTGGTTTTACCACTACCAGATTTACCAAGAATAACAACAAATTCTCCTGGAAGAATTTTTAAATTAATGTGGTTACAAATAAGTTGGTTGTTAATGCCATTTGTAACAGCTTTACAAACTTCTTTGAAGTAAATATCATACTTTTTAGCTTCAAATGAATTAAGTGATTTTCTTTTGGGGTGCTTTTTATTTAACTTTTTAATTAATTTAGATGCTTTTTTACTAACAGCAAAATCTGATGATTTTAAGTAAATATCAAACTTTTTATTCTCTTTTGTTTTTAAAACCATAAACAATCATTAAGTCATCTAAAAAAATACCCTGAGAACACTATTAGAAAATTTTAATTTTGCTAAATTACTAATGATGTAATTACATCCCGTAATGAACTGTCATTTTCAATCATTTTAGCAACTCTTTTAACTGCCATTAATACACTTGAATGACTTCTCTTAAATATTTTGCCAATTTGTTGAAATTGCATGTTGTACTTTTGACGCAAAAGGTAATTACACACATCACGAACACGGACAAGTTCTGCTTTACGATTTTCTGAAAGTACACTGTCCATAGGAACATTAAATCTACGGCAAACGTTCTCTATTAATAAATAAGGATCAAAGCTCTTTTTATGAAACTTCTCAAATTCTTCAAAAAGAATTTCTTTTAAATTTTCAGTATTTATTAAATTTTGTTTTGAAGTTTTAGCAAAAAATAATAACTTTGTTGCAATTCCATTTAAAGCACGCACATCGTTACCTGAAATTTGTGCTGCATCATGTCTTGCCTCATTAGTTATTTGGATGTTAGGATCTTTTTCTTTTAATTTAACAGTAAGTATTTCACAAAGTGAAGACAAATTATGCTTTTCTATCTTTAGTAATAATCCTGATTTAAAGCGAGAAATCATTCTTGCATCAATATCAATTAGTTCATCAGGAGCCTTATCAGAAACTAAAACAATTTGTTTTTTATTTAGAACTAGGTTATTAAAAATATTGAAAAGAATTTCTAAGGTTTTTTCTTTTCTGCCAAATATTTGAGTGTCATCTATTAAAACTAAATCTAAATTTTCATAATTTTTTTTTAGTTTTTCTATACCTTTATCCCTTTGATAAAAAGCATCAACAACTTCTTGGGCAAAATCACTTGAAACAACATACTTTACTCTGGCATTTGGAAAATTACGAAATTTTTCATTTCCTATTGCTTGTAGTAGGTGAGTTTTACCAAGACCGGTTTCTCCGTAAATAAAAAGCGGTGAAAATTCGTTATCTTGAGTTTCAGCTAATCTAACGCCTGCTTCATAAGCTCTTTTATTTCCTTCACTAATTACAAAGTTTTGAAAAGTATAGTTTTTACTCAATCCAGAATTTTGATAAAGAGTATCACGACTATTTTCTTCTAATTTTGCTAAGTTAAAAAAGAAATCTTGCTCATTAACAAAATTAACACTTTTAATTCCTTCATACAAACTTTCAGCTAAATGAATAATTTCAGAATTATCATTTAAGGAATTACGAGCAAATTCATTTTCTAAAAGAATTAAAAGAACATTATTTTTAAATACGAAACGATTAATGTCTTTAATGTATTTATCATGAAAACCTATTGTTTTTTCATAATGCTTTTTTAATAGAGATTTAAAGGCATTAAATTGTTCCATTATTCTTCTATAACATTGTCAAGAATGATAGTTAAAATTCTCGAAATTGGGATATTAACTGCTTTGGAGTAATTTCTAACTTTTTGTCATACTCTTTGACTTGTATAGAAGTGTACACCTGTATCTAGTTTTTCTTGGCGTTCAACAGGAACTATTCCTGGTATTTTTGTTTTAGGTTGGGGAGGAATAGGCTGTGGTTGTGTGAATTGTTGTTGAAAATTTTGATTTTTTTGCTGTAAGAAACCATTATTATGATATTGAAAATTTTGTTCCTCTTTAAAATATCTCTCTTTTTTTGGTTTTCCAGAAAAATTTGATGAAAAAGATTTTTCTTCATTTCAATTTTCAAGATTATTTTCATTTTGTTGATTTATTTGCTCAGGCTGTTGAAATGAATTATTTTTTGATCAAAAAGATTTTGGAAAGGTTTTTTCAAAAGCAGATAAAGGTCCAAAATCAAATGAAGATGAATCTTTGTCAAAAGATGTTTCTTCTCTTTTTGACAAATTTTGTTTTTGATTAAACTTATTTTTATTTTGGGGTGTTACTTTTTCTTTTATGGAAAACAAATCTTCTTCTAAAAGACTTTGTTCTGGGTCATCATCTTGTGCTAAATCAAAGAAAAAACGTTTCTTTTTGTTATTAATGGACATTGTAATTTGCTAAATTTAGGATTTCTTTTGTTATTTCTAAATACTCATTTAGATATTTTTTACTTGGTGATGATACTAATGATATTGGCAATTTTTCATAACCTACAGCTGCTGATGATTTTGATGTCAGAGAAACAAAATTTTTAGAAAAAGCTACATTATTTTTTTTAGCTTTTGTTTTAGCTAAATCTATTACTTCATTATGAAGACGAGTACGAACGTTAACTTTTGTAGGAACTAAAATAGTTTTAAGATTTGTATTTTTTTCCTTAAATGTATCTATTGTTTCAACTATTCTCATCAAACCTAGCATCGAATATTGATCTGGTTCAAAGGGAATAACTATGACATCTGATAAACTCATTGCAGTAGAAACTAAAGTTGCCATATTTGGTGGTGTATCTAATAAAACAAATTCATATCTTTTTGCTAGTTGCTTAACTATTTCTGCTATATCTGAGGCCTTATATTTTTTACGTGATATGTCTATATCAGCAAAATTAAGTTCAAAATTACAAGGAAGAATATCAAGTCCCTCATATACAGATAGCAAGCAATCATCTATTTCAATGAAATTATTTGAACCACTGAATTTTGGAACCTTCAACAAAATGTCAATTAACGTGTTATTCAATCTTTCAGGGTTTTGTCCAAATGATGCAGAAACATTCCCCTGCCCGTCAAGATCAAGAATGACTTTTCGCCTTTCTGGACAAAGTTTAACCAATGATCCTGCAACATTAGTTGCCATTGTAGTTTTTAATACGCCGCCTTTATTATTTACAAAAGAAATGATCATATATTTAAATGATTATAATATTTCTTTAATACTAAAAAAATAC", "length": 579504, "complete": true, "type": "contig", "topology": "circular", "simple_id": "contig_1", "orig_id": "GCA_000292445.1|CP003771.1", "orig_description": "HMT-616 Mycoplasmoides genitalium M6282" } ], "run": { "start": "2026-01-29 10:42:29", "end": "2026-01-29 10:49:24", "duration": "6.91 min" }, "version": { "bakta": "1.11.4", "db": { "version": "6.0", "type": "full" } } }