{ "genome": { "genus": "Mycoplasmoides", "species": "genitalium", "strain": "M2288", "taxon": "Mycoplasmoides genitalium M2288", "complete": true, "gram": "?", "translation_table": 11 }, "stats": { "size": 579558, "gc": 0.3167482805862399, "n_ratio": 0.0, "n50": 579558, "n90": 579558, "coding_ratio": 0.7903937138301947 }, "features": [ { "type": "cds", "sequence": "contig_1", "start": 686, "stop": 1828, "strand": "+", "frame": 2, "gene": "dnaN", "product": "DNA polymerase III subunit beta", "db_xrefs": [ "COG:COG0592", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0005737", "GO:0006260", "GO:0006271", "GO:0008408", "GO:0009360", "KEGG:K02338", "RefSeq:WP_014893888.1", "SO:0001217", "UniParc:UPI00027B32B2", "UniRef:UniRef100_UPI00027B32B2", "UniRef:UniRef50_P47247", "UniRef:UniRef90_P47247" ], "nt": "ATGAAAATATTAATTAATAAAAGTGAATTGAATAAAATTTTGAAAAAAATGAATAACGTTATTATTTCCAATAACAAAATAAAACCACATCATTCATATTTTTTAATAGAGGCAAAAGAAAAAGAAATAAACTTTTATGCTAACAATGAATACTTTTCTGTCAAATGTAATTTAAATAAAAATATTGATATTCTTGAACAAGGCTCCTTAATTGTTAAAGGAAAAATTTTTAACGATCTTATTAATGGCATAAAAGAAGAGATTATTACTATTCAAGAAAAAGATCAAACACTTTTGGTTAAAACAAAAAAAACAAGTATTAATTTAAACACAATTAATGTGAATGAATTTCCAAGAATAAGGTTTAATGAAAAAAACGATTTAAGTGAATTTAATCAATTCAAAATAAATTATTCACTTTTAGTAAAAGGCATTAAAAAAATTTTTCACTCAGTTTCAAATAATCGTGAAATATCTTCTAAATTTAATGGAGTAAATTTCAATGGATCCAATGGAAAAGAAATATTTTTAGAAGCTTCTGACACTTATAAACTATCTGTTTTTGAGATAAAGCAAGAAACAGAACCATTTGATTTCATTTTGGAGAGTAATTTACTTAGTTTCATTAATTCTTTTAATCCTGAAGAAGATAAATCTATTGTTTTTTATTACAGAAAAGATAATAAAGATAGCTTTAGTACAGAAATGTTGATTTCAATGGATAACTTTATGATTAGTTACACATCGGTTAATGAAAAATTTCCAGAGGTAAACTACTTTTTTGAATTTGAACCTGAAACTAAAATAGTTGTTCAAAAAAATGAATTAAAAGATGCACTTCAAAGAATTCAAACTTTGGCTCAAAATGAAAGAACTTTTTTATGCGATATGCAAATTAACAGTTCTGAATTAAAAATAAGAGCTACTGTTAATAATATCGGAAATTCTCTTGAGGAAATTTCTTGTCTTAAATTTGAAGGTTATAAACTTAATATTTCTTTTAACCCAAGTTCTCTATTAGATCACATAGAGTCTTTTGAATCAAATGAAATAAATTTTGATTTCCAAGGAAATAGTAAGTATTTTTTGATAACCTCTAAAAGTGAACCTGAACTTAAGCAAATATTGGTTCCTTCAAGATAA", "aa": "MKILINKSELNKILKKMNNVIISNNKIKPHHSYFLIEAKEKEINFYANNEYFSVKCNLNKNIDILEQGSLIVKGKIFNDLINGIKEEIITIQEKDQTLLVKTKKTSINLNTINVNEFPRIRFNEKNDLSEFNQFKINYSLLVKGIKKIFHSVSNNREISSKFNGVNFNGSNGKEIFLEASDTYKLSVFEIKQETEPFDFILESNLLSFINSFNPEEDKSIVFYYRKDNKDSFSTEMLISMDNFMISYTSVNEKFPEVNYFFEFEPETKIVVQKNELKDALQRIQTLAQNERTFLCDMQINSSELKIRATVNNIGNSLEEISCLKFEGYKLNISFNPSSLLDHIESFESNEINFDFQGNSKYFLITSKSEPELKQILVPSR", "aa_hexdigest": "a41d0cdf8f2b7ff6181a9a4ecc067eb0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B32B2", "ncbi_nrp_id": "WP_014893888.1", "uniref100_id": "UniRef100_UPI00027B32B2", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B32B2", "RefSeq:WP_014893888.1", "UniRef:UniRef100_UPI00027B32B2" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B32B2", "uniref90_id": "UniRef90_P47247" }, "psc": { "uniref90_id": "UniRef90_P47247", "gene": "dnaN", "product": "DNA polymerase III subunit beta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_P47247", "cog_id": "COG0592", "cog_category": "L", "kegg_orthology_id": "K02338", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0005737", "GO:0006260", "GO:0006271", "GO:0008408", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_P47247", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47247" ], "product": "Beta sliding clamp" }, "genes": [ "dnaN" ], "id": "JPCNHPFPBM_43", "locus": "GCA000292505_00001" }, { "type": "cds", "sequence": "contig_1", "start": 1828, "stop": 2073, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00226.36" ], "nt": "ATGAATCTTTACGATCTTTTAGAACTACCAACTACAGCATCAATAAAAGAAATAAAAATTGCTTATAAAAGATTAGCAAAGCGTTATCACCCTGATGTAAATAAATTAGGTTCGCAAACTTTTGTTGAAATTAATAATGCTTATTCAATATTAAGTGATCCTAACCAAAAGGAAAAATATGATTCAATGCTGAAAGTTAATGATTTTCAAAATCGCATCAAAAATTTAGATATTAGTGTTAGATGA", "aa": "MNLYDLLELPTTASIKEIKIAYKRLAKRYHPDVNKLGSQTFVEINNAYSILSDPNQKEKYDSMLKVNDFQNRIKNLDISVR", "aa_hexdigest": "bd68886341465ed340ba720a06a2fb0d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00226.36", "name": "DnaJ", "length": 63, "aa_cov": 0.7407407407407407, "hmm_cov": 1.0, "evalue": 1.7382613911077364e-22, "score": 74.42524719238281, "start": 2, "stop": 61 } ], "seq_stats": { "molecular_weight": 9475.772099999996, "isoelectric_point": 9.221831703186034 }, "id": "JPCNHPFPBM_44", "locus": "GCA000292505_00002" }, { "type": "cds", "sequence": "contig_1", "start": 2605, "stop": 2760, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGTAGTTAATAAAGTAAACAAAAGACTGCGTATTTTTTCAAGCTTTTTTGAGAACGATAAATCTAAATTATGGTTCCTTGTTCCAAACGATAAACAAAGTAATCCTAATAAGGGCGTTTTTAACTATAAAACTCAGCACTTTATTGATTAA", "aa": "MKVVNKVNKRLRIFSSFFENDKSKLWFLVPNDKQSNPNKGVFNYKTQHFID", "aa_hexdigest": "7ccff5a9fba33d568e382fcbb1e0eb7b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6148.016900000002, "isoelectric_point": 10.092283821105958 }, "id": "JPCNHPFPBM_45", "locus": "GCA000292505_00003" }, { "type": "cds", "sequence": "contig_1", "start": 2845, "stop": 2979, "strand": "+", "frame": 1, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920FT50" ], "nt": "ATGGAAGAAAATAACAAAGCAAATATCTATGACTCTAGTAGCATTAAGGTCCTTGAAGGACTTGAGGCTGTTAGAAAACGCCCTGGAATGTACATTGGTTCTACTGGCGAAGAAGGTTTGCATCACATGATCTGA", "aa": "MEENNKANIYDSSSIKVLEGLEAVRKRPGMYIGSTGEEGLHHMI", "aa_hexdigest": "838391e1dd8186643f04d3bd2b89c52e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A920FT50", "query_cov": 0.9090909090909091, "subject_cov": 0.8, "identity": 0.7, "score": 58.5, "evalue": 1.34e-10, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0A920FT50" }, "pscc": { "uniref50_id": "UniRef50_A0A920FT50", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920FT50" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "JPCNHPFPBM_46", "locus": "GCA000292505_00004" }, { "type": "cds", "sequence": "contig_1", "start": 3010, "stop": 3255, "strand": "+", "frame": 1, "gene": null, "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380EBM1" ], "nt": "ATGGGAGGTTTTGCCAGTTTTGTTAAGCTTACCCTTGAAGATAATTTTGTTACCCGTGTAGAGGATGATGGAAGAGGGATACCTGTTGATATCCATCCTAAGACTAATCGTTCTACAGTTGAAACAGTTTTTACAGTTCTACACGCTGGCGGTAAATTTGATAACGATAGCTATAAAGTGTCAGGTGGTTTACACGGTGTTGGTGCATCAGTTGTTAATGCGCTTAGTTCTTCTTTTAAAGTTTGA", "aa": "MGGFASFVKLTLEDNFVTRVEDDGRGIPVDIHPKTNRSTVETVFTVLHAGGKFDNDSYKVSGGLHGVGASVVNALSSSFKV", "aa_hexdigest": "930d6e6912b2909e276c273e3dce4c94", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021B43CD8", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.568, "score": 93.2, "evalue": 2.53e-23, "valid": false, "product": "ATP-binding protein", "uniref50_id": "UniRef50_A0A380EBM1" }, "pscc": { "uniref50_id": "UniRef50_A0A380EBM1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380EBM1" ], "product": "DNA topoisomerase (ATP-hydrolyzing)" }, "genes": [], "id": "JPCNHPFPBM_47", "locus": "GCA000292505_00005" }, { "type": "cds", "sequence": "contig_1", "start": 3319, "stop": 3513, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTCCAAGAAGGTAACTCTGAAAAAGAGCATGGAACAATTGTTGAGTTTGTTCCTGATTTCTCTGTAATGGAAAAGAGTGATTACAAACAAACTGTAATTGTAAGCAGACTCCAGCAATTAGCTTTTTTAAACAAGGGAATAAGAATTGACTTTGTTGATAATCGTAAACAAAACCCACAGTCTTTTTCTTGA", "aa": "MVQEGNSEKEHGTIVEFVPDFSVMEKSDYKQTVIVSRLQQLAFLNKGIRIDFVDNRKQNPQSFS", "aa_hexdigest": "5dc3e325e8cc02961f18c22b3972012f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7404.266600000002, "isoelectric_point": 5.678067588806152 }, "id": "JPCNHPFPBM_48", "locus": "GCA000292505_00006" }, { "type": "cds", "sequence": "contig_1", "start": 3529, "stop": 4557, "strand": "+", "frame": 1, "gene": "gyrB", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit B", "db_xrefs": [ "COG:COG0187", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0034335", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P22447", "UniRef:UniRef90_P22447" ], "nt": "TTGGTTGAATATATCCACCACCTAAACAACGAAAAAGAACCACTTTTTAATGAAGTTATTGCTGATGAAAAAACTGAAACTGTAAAAGCTGTTAATCGTGATGAAAACTACACAGTAAAGGTTGAAGTTGCTTTTCAATATAACAAAACATACAACCAATCAATTTTCAGTTTTTGTAACAACATTAATACTACAGAAGGTGGAACCCATGTGGAAGGTTTTCGTAATGCACTTGTTAAGATCATTAATCGCTTTGCTGTTGAAAATAAATTCCTAAAAGATAGTGATGAAAAGATTAACCGTGATGATGTTTGTGAAGGATTAACTGCTATTATTTCCATTAAACACCCAAACCCACAATATGAAGGACAAACTAAAAAGAAGTTAGGTAATACTGAGGTAAGACCTTTAGTTAATAGTGTTGTTAGTGAAATCTTTGAACGCTTCATGTTAGAAAACCCACAAGAAGCAAACGCTATCATCAGAAAAACACTTTTAGCTCAAGAAGCGAGAAGAAGAAGTCAAGAGGCTAGGGAGTTAACTCGTCGTAAATCACCTTTTGATAGTGGTTCATTACCAGGTAAATTAGCTGATTGTACAACCAGAGATCCTTCGATTAGTGAACTTTACATTGTTGAGGGTGATAGTGCTGGTGGCACTGCTAAAACAGGAAGAGATCGTTATTTTCAAGCTATCTTACCCTTAAGAGGAAAGATTTTAAACGTTGAAAAATCTAACTTTGAACAAATCTTTAATAATGCAGAAATTTCTGCATTAGTGATGGCAATAGGCTGTGGGATTAAACCTGATTTTGAACTTGAAAAACTTAGATATAGCAAGATTGTGATCATGACAGATGCTGATGTTGATGGTGCACACATAAGAACACTTCTCTTAACTTTCTTTTTTCGCTTTATGTATCCTTTGGTTGAACAAGGCAATATTTTTATTGCTCAACCCCCACTTTATAAAGTGTCATATTCCCATAAGGATTTATACATGCACACTGATGTTCAACTTGAACAGTGA", "aa": "MVEYIHHLNNEKEPLFNEVIADEKTETVKAVNRDENYTVKVEVAFQYNKTYNQSIFSFCNNINTTEGGTHVEGFRNALVKIINRFAVENKFLKDSDEKINRDDVCEGLTAIISIKHPNPQYEGQTKKKLGNTEVRPLVNSVVSEIFERFMLENPQEANAIIRKTLLAQEARRRSQEARELTRRKSPFDSGSLPGKLADCTTRDPSISELYIVEGDSAGGTAKTGRDRYFQAILPLRGKILNVEKSNFEQIFNNAEISALVMAIGCGIKPDFELEKLRYSKIVIMTDADVDGAHIRTLLLTFFFRFMYPLVEQGNIFIAQPPLYKVSYSHKDLYMHTDVQLEQ", "aa_hexdigest": "a401d9d9670848dc5b513361b388c93d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3D5R7J3", "query_cov": 1.0, "subject_cov": 0.8260869565217391, "identity": 0.5379999999999999, "score": 366.0, "evalue": 3.5e-122, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_K2BJ35" }, "genes": [ "gyrB" ], "truncated": "both", "pseudogene": { "start": 3229, "stop": 4797, "observations": { "mutation": [ 3253, 3511, 4555, 4633 ], "stop": [ 3253, 3511, 4555, 4633 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P22447", "query_cov": 1.0, "subject_cov": 0.5261538461538462, "identity": 0.997, "score": 670.0, "evalue": 2.79e-238, "gene_start": 1, "gene_end": 342, "reference_start": 229, "reference_end": 570, "reference_sequence": "MEENNKANIYDSSSIKVLEGLEAVRKRPGMYIGSTGEEGLHHMIWEIVDNSIDEAMGGFASFVKLTLEDNFVTRVEDDGRGIPVDIHPKTNRSTVETVFTVLHAGGKFDNDSYKVSGGLHGVGASVVNALSSSFKVWVFRQNKKYFLSFSDGGKVIGDLVQEGNSEKEHGTIVEFVPDFSVMEKSDYKQTVIVSRLQQLAFLNKGIRIDFVDNRKQNPQSFSWKYDGGLVEYIHHLNNEKEPLFNEVIADEKTETVKAVNRDENYTVKVEVAFQYNKTYNQSIFSFCNNINTTEGGTHVEGFRNALVKIINRFAVENKFLKDSDEKINRDDVCEGLTAIISIKHPNPQYEGQTKKKLGNTEVRPLVNSVVSEIFERFMLENPQEANAIIRKTLLAQEARRRSQEARELTRRKSPFDSGSLPGKLADCTTRDPSISELYIVEGDSAGGTAKTGRDRYFQAILPLRGKILNVEKSNFEQIFNNAEISALVMAIGCGIKPDFELEKLRYSKIVIMTDADVDGAHIRTLLLTFFFRFMYPLVEQGNIFIAQPPLYKVSYSHKDLYMHTDVQLEQWKSQNPNVKFGLQRYKGLGEMDALQLWETTMDPKVRTLLKVTVEDASIADKAFSLLMGDEVPPRREFIEKNARSVKNIDI" }, "paralog": false, "identity": 0.9923371647509579, "score": 1017.0, "evalue": 0.0, "description": "Internal stop codon at 3253, 3511, 4555, 4633. Nonsense mutation around 3253, 3511, 4555, 4633.", "psc": { "uniref90_id": "UniRef90_P22447", "gene": "gyrB", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit B", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P22447", "cog_id": "COG0187", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0034335", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P22447", "EC:5.6.2.2", "UniRef:UniRef50_P22447", "COG:COG0187", "COG:L", "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0034335", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P22447", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P22447" ], "product": "DNA gyrase subunit B" } }, "id": "JPCNHPFPBM_49", "locus": "GCA000292505_00007" }, { "type": "cds", "sequence": "contig_1", "start": 4645, "stop": 4797, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCCTAAGGTTAGAACATTGTTAAAAGTTACTGTTGAAGATGCTTCTATTGCTGATAAAGCTTTTTCACTGTTGATGGGTGATGAAGTTCCCCCAAGAAGAGAATTTATTGAAAAAAATGCTCGTAGTGTTAAAAACATTGATATTTAA", "aa": "MDPKVRTLLKVTVEDASIADKAFSLLMGDEVPPRREFIEKNARSVKNIDI", "aa_hexdigest": "005320cf15fac2ee99e015a052764196", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A926IWG4", "query_cov": 1.0, "subject_cov": 0.8333333333333334, "identity": 0.68, "score": 68.2, "evalue": 3.33e-14, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0A926IWG4" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5657.521399999999, "isoelectric_point": 6.141760063171386 }, "id": "JPCNHPFPBM_50", "locus": "GCA000292505_00008" }, { "type": "cds", "sequence": "contig_1", "start": 4812, "stop": 7322, "strand": "+", "frame": 3, "gene": "gyrA", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit A", "db_xrefs": [ "COG:COG0188", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0009330", "GO:0034335", "KEGG:K02469", "RefSeq:WP_014894525.1", "SO:0001217", "UniParc:UPI00027B42CA", "UniRef:UniRef100_UPI00027B42CA", "UniRef:UniRef50_P47719", "UniRef:UniRef90_P47250" ], "nt": "ATGGCAAAGCAACAAGATCAAGTAGATAAGATTCGTGAAAACTTAGACAATTCAACTGTCAAAAGTATTTCATTAGCAAATGAACTTGAGCGTTCATTCATGGAATATGCTATGTCAGTTATTGTTGCTCGTGCTTTACCTGATGCTAGAGATGGACTTAAACCAGTTCATCGTCGTGTTCTTTATGGTGCTTATATTGGTGGCATGCACCATGATCGTCCTTTTAAAAAGTCTGCGAGGATTGTTGGTGATGTAATGAGTAAATTCCACCCTCATGGTGATATGGCAATATATGACACCATGTCAAGAATGGCTCAAGACTTTTCATTAAGATACCTTTTAATTGATGGTCATGGTAATTTTGGTTCTATAGATGGTGATAGACCTGCTGCACAACGTTATACAGAAGCAAGATTATCTAAACTTGCAGCAGAACTTTTAAAAGATATTGATAAAGATACAGTTGACTTTATTGCTAATTATGATGGTGAGGAAAAAGAACCAACTGTTCTACCAGCAGCTTTCCCTAACTTACTTGCAAATGGTTCTAGTGGGATTGCAGTTGGAATGTCAACATCTATTCCTTCCCATAATCTCTCTGAATTAATTGCGGGTTTAATCATGTTAATTGATAATCCTCAATGCACTTTTCAAGAATTATTAACTGTAATTAAAGGACCTGATTTTCCAACAGGAGCTAACATTATCTACACAAAAGGAATTGAAAGCTACTTTGAAACAGGTAAAGGCAATGTAGTAATTCGTTCTAAAGTTGAGATAGAACAATTGCAAACAAGAAGTGCATTAGTTGTAACTGAAATTCCTTACATGGTTAACAAAACTACCTTAATTGAAAAGATTGTAGAACTTGTTAAAGCTGAAGAGATTTCAGGAATTGCTGATATCCGTGATGAATCCTCTCGAGAAGGAATAAGGTTAGTGATTGAAGTAAAACGCGACACTGTACCTGAAGTTTTATTAAATCAACTTTTTAAATCAACAAGATTACAAGTACGCTTCCCTGTTAATATACTTGCTTTAGTTAAAGGAGCTCCTGTACTTCTCAACATGAAACAAGCTTTGGAAGTATATCTTGATCATCAAATTGATGTTCTTGTTAGAAAAACAAAGTTTGTGCTTAATAAACAACAAGAACGTTATCACATTTTAAGCGGACTTTTAATTGCTGCTTTAAATATTGATGAGGTTGTTGCAATTATTAAAAAATCAGCAAATAACCAGGAAGCAATTAATACATTAAATACAAAGTTTAAGCTTGATGAAATTCAAGCTAAAGCAGTTCTTGACATGCGTTTAAGGAGCTTAAGCGTACTTGAAGTTAACAAACTTCAAACTGAACAAAAAGAGTTAAAAGATTCAATTGAATTTTGTAAGAAAGTGTTAGCTGATCAAAAATTACAGCTAAAAATAATCAAAGAGGAATTGCAAAAAATCAATGATCAGTTTGGTGATGAAAGAAGAAGTGAAATTCTCTATGATATCTCTGAGGAAATTGATGATGAATCATTGATAAAAGTTGAGAATGTAGTGATAACTATGTCTACAAATGGTTATCTAAAAAGGATTGGAGTTGATGCTTATAATCTTCAACATCGTGGTGGAGTTGGGGTTAAAGGGCTAACTACTTATGTTGATGATAGTATTAGTCAATTATTGGTCTGTTCAACTCACTCTGACTTATTATTTTTTACTGATAAGGGTAAGGTTTATAGAATTAGAGCTCATCAAATTCCCTATGGTTTTAGAACAAATAAAGGTATTCCCGCTGTTAACTTAATCAAAATTGAAAAGGATGAAAGAATTTGTTCATTGTTATCTGTTAATAACTATGATGATGGTTATTTCTTTTTCTGTACTAAAAATGGAATTGTTAAAAGAACGAGCTTGAATGAATTCATCAACATCTTAAGTAATGGTAAGCGGGCTATATCTTTTGATGATAATGACACTTTGTATTCAGTAATTAAAACCCACGGAAATGATGAGATTTTTATTGGTTCTACCAATGGATTTGTTGTTCGATTCCATGAAAATCAACTCAGAGTTCTTTCAAGAACAGCAAGAGGTGTATTTGGTATCAGTTTAAATAAAGGAGAATTTGTTAATGGACTATCAACTTCAAGCAACGGTAGCTTACTTTTATCAGTCGGTCAAAATGGAATAGGTAAATTAACGAGCATAGATAAATATAGGCTCACAAAACGTAATGCTAAGGGAGTTAAAACTCTAAGGGTTACTGATAGAACAGGCCCTGTTGTTACAACAACCACTGTTTTTGGTAATGAGGATCTTTTAATGATTTCCTCTGCTGGTAAAATTGTGCGTACCAGTTTACAAGAACTTTCAGAACAAGGTAAAAACACTTCTGGTGTTAAGTTAATTAGATTAAAAGATAATGAACGTTTAGAAAGAGTAACTATCTTTAAAGAAGAGTTAGAAGACAAAGAAATGCAACTAGAAGATGTTGGATCCAAACAAATTACGCAATAA", "aa": "MAKQQDQVDKIRENLDNSTVKSISLANELERSFMEYAMSVIVARALPDARDGLKPVHRRVLYGAYIGGMHHDRPFKKSARIVGDVMSKFHPHGDMAIYDTMSRMAQDFSLRYLLIDGHGNFGSIDGDRPAAQRYTEARLSKLAAELLKDIDKDTVDFIANYDGEEKEPTVLPAAFPNLLANGSSGIAVGMSTSIPSHNLSELIAGLIMLIDNPQCTFQELLTVIKGPDFPTGANIIYTKGIESYFETGKGNVVIRSKVEIEQLQTRSALVVTEIPYMVNKTTLIEKIVELVKAEEISGIADIRDESSREGIRLVIEVKRDTVPEVLLNQLFKSTRLQVRFPVNILALVKGAPVLLNMKQALEVYLDHQIDVLVRKTKFVLNKQQERYHILSGLLIAALNIDEVVAIIKKSANNQEAINTLNTKFKLDEIQAKAVLDMRLRSLSVLEVNKLQTEQKELKDSIEFCKKVLADQKLQLKIIKEELQKINDQFGDERRSEILYDISEEIDDESLIKVENVVITMSTNGYLKRIGVDAYNLQHRGGVGVKGLTTYVDDSISQLLVCSTHSDLLFFTDKGKVYRIRAHQIPYGFRTNKGIPAVNLIKIEKDERICSLLSVNNYDDGYFFFCTKNGIVKRTSLNEFINILSNGKRAISFDDNDTLYSVIKTHGNDEIFIGSTNGFVVRFHENQLRVLSRTARGVFGISLNKGEFVNGLSTSSNGSLLLSVGQNGIGKLTSIDKYRLTKRNAKGVKTLRVTDRTGPVVTTTTVFGNEDLLMISSAGKIVRTSLQELSEQGKNTSGVKLIRLKDNERLERVTIFKEELEDKEMQLEDVGSKQITQ", "aa_hexdigest": "0d3097848124dd8414410022f4559879", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B42CA", "ncbi_nrp_id": "WP_014894525.1", "uniref100_id": "UniRef100_UPI00027B42CA", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B42CA", "RefSeq:WP_014894525.1", "UniRef:UniRef100_UPI00027B42CA" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B42CA", "uniref90_id": "UniRef90_P47250" }, "psc": { "uniref90_id": "UniRef90_P47250", "gene": "gyrA", "product": "DNA topoisomerase (ATP-hydrolyzing) subunit A", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P47719", "cog_id": "COG0188", "cog_category": "L", "kegg_orthology_id": "K02469", "go_ids": [ "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0009330", "GO:0034335" ] }, "pscc": { "uniref50_id": "UniRef50_P47719", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47719" ], "product": "DNA gyrase subunit A" }, "genes": [ "gyrA" ], "id": "JPCNHPFPBM_51", "locus": "GCA000292505_00009" }, { "type": "cds", "sequence": "contig_1", "start": 7294, "stop": 7857, "strand": "+", "frame": 1, "gene": "serS", "product": "serine--tRNA ligase", "db_xrefs": [ "COG:COG0172", "COG:J", "EC:6.1.1.11", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0005737", "GO:0006434", "GO:0016260", "KEGG:K01875", "SO:0001217", "UniRef:UniRef50_P47251", "UniRef:UniRef90_P47251" ], "nt": "ATGTTGGATCCAAACAAATTACGCAATAACTATGATTTCTTTAAAAAGAAACTGTTAGAAAGAAATGTAAATGAGCAATTATTAAATCAGTTTATTCAAACTGATAAACTAATGCGCAAAAACTTGCAACAACTTGAACTTGCTAACCAAAAACAAAGCTTGTTGGCAAAACAAGTTGCTAAGCAAAAAGATAATAAAAAGCTATTAGCTGAATCAAAAGAACTTAAGCAGAAGATTGAAAACTTAAATAGTGCTTATAAAGATTCACAAAACATTAGTCAGGATTTACTTCTAAATTTTCCTAATATTGCTCATGAATCAGTTCCTGTTGGTAAAAATGAATCAGCAAACTTAGAACTCCTTAAAGAAGGGAGAAAACCAGTTTTTGATTTCAAACCTTTACCACATTGGGAGTTATGTGAAAAGTTAAATTTAGTTGCTTTTGATAAAGCTACTAAGATTAGTGGAACTAGGTTTGTTGCATATACAGATAAAGCAGCTAAACTGCTTAGAGCGATAACTAATCTAATGATTGACCTTAATAAAAGCAAGTATCAAGAATGA", "aa": "MLDPNKLRNNYDFFKKKLLERNVNEQLLNQFIQTDKLMRKNLQQLELANQKQSLLAKQVAKQKDNKKLLAESKELKQKIENLNSAYKDSQNISQDLLLNFPNIAHESVPVGKNESANLELLKEGRKPVFDFKPLPHWELCEKLNLVAFDKATKISGTRFVAYTDKAAKLLRAITNLMIDLNKSKYQE", "aa_hexdigest": "a4a983d104cb3c2bd0ef6dccfffcae96", "start_type": "ATG", "rbs_motif": null, "genes": [ "serS" ], "truncated": "3-prime", "pseudogene": { "start": 7294, "stop": 8160, "observations": { "mutation": [ 7855 ], "stop": [ 7855 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47251", "query_cov": 1.0, "subject_cov": 0.44844124700239807, "identity": 0.9890000000000001, "score": 345.0, "evalue": 1.46e-116, "gene_start": 1, "gene_end": 187, "reference_start": 1, "reference_end": 187, "reference_sequence": "MLDPNKLRNNYDFFKKKLLERNVNEQLLNQFIQTDKLMRKNLQQLELANQKQSLLAKQVAKQKDNKKLLAESKELKQKIENLNNAYKDSQNISQDLLLNFPNIAHESVPVGKNESANLELLKEGRKPVFDFKPLPHRELCEKLNLVAFDKATKISGTRFVAYTDKAAKLLRAITNLMIDLNKSKYQEWNLPVVINELSLRSTGQLPKFKDDVFKLENTRYYLSPTLEVQLINLHANEIFNEEDLPKYYTATGINFRQEAGSAGKQTKGTIRLHQFQKTELVKFCKPENAINELEAMVRDAEQILKALKLPFRRLLLCTGDMGFSAEKTYDLEVWMAASNEYREVSSCSSCGDFQARRAMIRYKDINNGKNSYVATLNGTALSIDRIFAAILENFQTKDGKILIPQALKKYLDFDTIK" }, "paralog": false, "identity": 0.9895833333333334, "score": 560.0, "evalue": 1.16e-203, "description": "Internal stop codon at 7855. Nonsense mutation around 7855.", "psc": { "uniref90_id": "UniRef90_P47251", "gene": "serS", "product": "serine--tRNA ligase", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_P47251", "cog_id": "COG0172", "cog_category": "J", "kegg_orthology_id": "K01875", "go_ids": [ "GO:0000166", "GO:0004828", "GO:0005524", "GO:0005737", "GO:0006434", "GO:0016260" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47251", "EC:6.1.1.11", "UniRef:UniRef50_P47251", "COG:COG0172", "COG:J", "KEGG:K01875", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0005737", "GO:0006434", "GO:0016260" ] }, "pscc": { "uniref50_id": "UniRef50_P47251", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47251" ], "product": "Serine--tRNA ligase" } }, "id": "JPCNHPFPBM_52", "locus": "GCA000292505_00010" }, { "type": "cds", "sequence": "contig_1", "start": 8107, "stop": 8295, "strand": "+", "frame": 1, "gene": null, "product": "aminoacyl--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0032988E16" ], "nt": "TTGCATCAGTTTCAAAAAACTGAGTTAGTTAAGTTTTGTAAACCTGAAAATGCTATCAATGAATTGGAAGCAATGGTTAGAGATGCTGAACAAATCTTAAAGGCACTTAAGTTACCTTTTAGAAGGTTATTGTTATGTACTGGTGATATGGGCTTTAGTGCTGAAAAAACATATGATCTTGAAGTTTGA", "aa": "MHQFQKTELVKFCKPENAINELEAMVRDAEQILKALKLPFRRLLLCTGDMGFSAEKTYDLEV", "aa_hexdigest": "e28a48f94f7fd7ff0c03f1b7e954a7db", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002F2627E6", "query_cov": 1.0, "subject_cov": 0.8857142857142857, "identity": 0.5479999999999999, "score": 73.6, "evalue": 5.18e-16, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_UPI0032988E16", "kegg_orthology_id": "K01875" }, "pscc": { "uniref50_id": "UniRef50_UPI0032988E16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0032988E16" ], "product": "aminoacyl--tRNA ligase-related protein" }, "genes": [], "id": "JPCNHPFPBM_53", "locus": "GCA000292505_00011" }, { "type": "cds", "sequence": "contig_1", "start": 8296, "stop": 8547, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "ATGGCAGCTAGCAATGAATATCGTGAAGTTTCTTCTTGTTCATCTTGTGGTGATTTTCAAGCAAGAAGAGCTATGATTCGTTACAAAGATATTAACAACGGTAAAAACAGTTATGTTGCTACTTTAAATGGAACAGCATTATCTATTGATAGAATTTTTGCTGCAATTCTAGAAAATTTTCAAACAAAAGATGGCAAAATTCTTATCCCACAAGCATTAAAAAAATACCTTGATTTTGACACAATCAAGTAA", "aa": "MAASNEYREVSSCSSCGDFQARRAMIRYKDINNGKNSYVATLNGTALSIDRIFAAILENFQTKDGKILIPQALKKYLDFDTIK", "aa_hexdigest": "dcc1f77d2836fa16e7d8406aa61056ff", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00345BB37E", "query_cov": 0.891566265060241, "subject_cov": 0.8222222222222222, "identity": 0.5539999999999999, "score": 89.4, "evalue": 1.17e-21, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_A0A3B8WPJ3", "kegg_orthology_id": "K01875" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 57, "aa_cov": 0.6867469879518072, "hmm_cov": 0.9649122807017544, "evalue": 2.082179734211332e-09, "score": 32.797603607177734, "start": 4, "stop": 60 } ], "seq_stats": { "molecular_weight": 9314.53189999999, "isoelectric_point": 9.001543235778808 }, "id": "JPCNHPFPBM_54", "locus": "GCA000292505_00012" }, { "type": "cds", "sequence": "contig_1", "start": 8551, "stop": 9183, "strand": "+", "frame": 1, "gene": "tmk", "product": "dTMP kinase", "db_xrefs": [ "COG:COG0125", "COG:F", "EC:2.7.4.9", "GO:0004798", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235", "KEGG:K00943", "RefSeq:WP_014894526.1", "SO:0001217", "UniParc:UPI00027B42CB", "UniRef:UniRef100_UPI00027B42CB", "UniRef:UniRef50_P47252", "UniRef:UniRef90_P47252" ], "nt": "ATGAATAAAGGTGTTTTTGTTGTTATTGAAGGAGTTGATGGAGCGGGCAAAACTGCTTTAATTGAAGGTTTTAAAAAACTTTATCCAACTAAGTTTTTGAACTATCAACTTACTTATACTAGAGAACCTGGTGGTACTTTGTTAGCTGAAAAAATTCGTCAACTTCTTTTAAATGAAACAATGGAACCTCTAACTGAAGCTTATTTGTTTGCCGCAGCTAGAACTGAACATATCAGTAAGCTAATTAAACCAGCAATTGAAAAAGAACAACTAGTTATTTCAGATAGATTTGTTTTCTCTAGTTTTGCATACCAAGGATTAAGCAAAAAAATAGGCATTGATACAGTAAAACAGATTAATCATCATGCGTTAAGAAATATGATGCCAAACTTTACCTTTATTTTGGATTGCAATTTTAAAGAAGTATTACAAAGGATGCAAAAGCGTGGTAATGATAATCTTCTTGATGAATTTATTAAAGGAAAGAATGATTTTGATACAGTTCGTTCTTATTATTTAAGCTTAGTTGATAAAAAAAACTGTTTCTTGATTAATGGTGATAATAAACAAGAACACCTAGAGAAATTTATTGAATTGTTAACAAGATGCTTACAACAACCCACGCATTACTAA", "aa": "MNKGVFVVIEGVDGAGKTALIEGFKKLYPTKFLNYQLTYTREPGGTLLAEKIRQLLLNETMEPLTEAYLFAAARTEHISKLIKPAIEKEQLVISDRFVFSSFAYQGLSKKIGIDTVKQINHHALRNMMPNFTFILDCNFKEVLQRMQKRGNDNLLDEFIKGKNDFDTVRSYYLSLVDKKNCFLINGDNKQEHLEKFIELLTRCLQQPTHY", "aa_hexdigest": "fbe4b416bbb5e4aaa99d062fedceb4ad", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B42CB", "ncbi_nrp_id": "WP_014894526.1", "uniref100_id": "UniRef100_UPI00027B42CB", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B42CB", "RefSeq:WP_014894526.1", "UniRef:UniRef100_UPI00027B42CB" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B42CB", "uniref90_id": "UniRef90_P47252" }, "psc": { "uniref90_id": "UniRef90_P47252", "gene": "tmk", "product": "dTMP kinase", "ec_ids": [ "2.7.4.9" ], "uniref50_id": "UniRef50_P47252", "cog_id": "COG0125", "cog_category": "F", "kegg_orthology_id": "K00943", "go_ids": [ "GO:0004798", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006227", "GO:0006233", "GO:0006235" ] }, "pscc": { "uniref50_id": "UniRef50_P47252", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47252" ], "product": "Thymidylate kinase" }, "genes": [ "tmk" ], "id": "JPCNHPFPBM_55", "locus": "GCA000292505_00013" }, { "type": "cds", "sequence": "contig_1", "start": 9156, "stop": 9323, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTACAACAACCCACGCATTACTAATCATTCAAAGAAAAGGTAGTTTCTTAAAACCTTTTCTTGATAATTATCTTACTAGTATTGTTTGTGAAAACAAAAATGGTTGCAAAAAGTGTATAAACTGTTTGGAAATTCTCAATAATAAATACAACAGCTTATATTGA", "aa": "MLTTTHALLIIQRKGSFLKPFLDNYLTSIVCENKNGCKKCINCLEILNNKYNSLY", "aa_hexdigest": "eaef039d4c156b9a8950d9e6f9012a48", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6339.452299999999, "isoelectric_point": 9.046606636047361 }, "id": "JPCNHPFPBM_56", "locus": "GCA000292505_00014" }, { "type": "cds", "sequence": "contig_1", "start": 9468, "stop": 9755, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGACTAGTTGAAGATAGTCCGATAAATAGTTATGGTATTTTTACAACTAAAAATGAAAGTTTAATTCTTTCCACTTTTTTAAGTAGAGTACAGAAAGTAGTTTTAAAAAAAGCTAGTAAAGTTCCTTTTAAAGTAAGCAAAAATGATCAAGAAATTATTACAAGTTTTTTTACTGTAGATGAACAAATTGAAGCAATTGAAAATGGAAGTTTTAACCGTTTCAAAATTATCTTAGATGCATGTTTAAACAAAAAAACAGGTACAGAACAAATTTATCATGCTTGA", "aa": "MRLVEDSPINSYGIFTTKNESLILSTFLSRVQKVVLKKASKVPFKVSKNDQEIITSFFTVDEQIEAIENGSFNRFKIILDACLNKKTGTEQIYHA", "aa_hexdigest": "1ad9da980b4fbfcd8b0643c6465d8fab", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10816.33169999999, "isoelectric_point": 8.75959339141846 }, "id": "JPCNHPFPBM_57", "locus": "GCA000292505_00015" }, { "type": "cds", "sequence": "contig_1", "start": 9923, "stop": 11059, "strand": "+", "frame": 2, "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "db_xrefs": [ "COG:COG0486", "COG:J", "EC:3.6.-.-", "GO:0002098", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0006400", "GO:0030488", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47254", "UniRef:UniRef90_P47254" ], "nt": "ATGAAAAGCGAAATTAATATTTTTGCACTAGCAACTGCACCTTTTAATAGTGCATTACATATTATTAGGTTTTCTGGTCCTGATGTTTATGAGATTTTAAACAAGATAACTAATAAAAAAATAACAAGAAAAGGGATGCAAATTCAACGCACATGGATAGTTGATGAAAACAATAAGCGAATTGATGATGTGCTATTATTTAAATTTGTCTCTCCAAATTCTTATACAGGAGAAGATTTAATTGAAATTTCTTGTCATGGTAACATGTTGATCGTTAATGAAATTTGCGCACTTCTTTTAAAAAAAGGAGGTGTTTATGCCAAACCTGGTGAATTTACCCAAAGGAGTTTTTTAAATGGAAAAATGAGTTTACAACAAGCTAGTGCTGTAAATAAATTGATTTTATCTCCTAACTTATTAGTTAAAGATATAGTCTTAAATAATTTAGCGGGTGAAATGGATCAACAATTAGAACAAATAGCTCAACAAGTTAATCAATTAGTAATGCAAATGGAAGTAAACATTGATTATCCAGAATATCTTGATGAACAAGTAGAACTATCAACTTTAAATAATAAAGTTAAATTGATTATTGAAAAGCTTAAAAGAATTATTGAAAATAGTAAACAACTCAAAAAACTTCACGATCCTTTTAAAATTGCCATTATAGGCGAAACTAATGTAGGTAAATCTTCTTTACTCAACGCTTTATTAAATCAAGATAAAGCGATAGTTTCAAATATTAAAGGTAGTACACGCGATGTTGTTGAAGGGGATTTCAATTTAAATGGTTATTTAATCAAGATCTTAGATACTGCAGGTATCCGTAAACATAAAAGTGGGCTTGAAAAAGCAGGAATTAAAAAAAGCTTTGAATCTATAAAGCAAGCTAATTTGGTTATTTATCTTTTAGATGCAACACATCCAAAGAAAGATCCTGAATTAATTAGTTTTTTTAAGAAAAATAAAAAGGATTTTTTTGTTTTCTATAACAAAAAAGATTTAATTACAAATAAGTTTGAAAATAGTATTTCTGCAAAGCAAAAAGATATTAAAGAATTAGTTGATTTATTAACTAAATATATTAACGAGTTTTATAAAAAAATAGATCAAAAAATCTATCTGATTGAAAATTGA", "aa": "MKSEINIFALATAPFNSALHIIRFSGPDVYEILNKITNKKITRKGMQIQRTWIVDENNKRIDDVLLFKFVSPNSYTGEDLIEISCHGNMLIVNEICALLLKKGGVYAKPGEFTQRSFLNGKMSLQQASAVNKLILSPNLLVKDIVLNNLAGEMDQQLEQIAQQVNQLVMQMEVNIDYPEYLDEQVELSTLNNKVKLIIEKLKRIIENSKQLKKLHDPFKIAIIGETNVGKSSLLNALLNQDKAIVSNIKGSTRDVVEGDFNLNGYLIKILDTAGIRKHKSGLEKAGIKKSFESIKQANLVIYLLDATHPKKDPELISFFKKNKKDFFVFYNKKDLITNKFENSISAKQKDIKELVDLLTKYINEFYKKIDQKIYLIEN", "aa_hexdigest": "1130597853a283bfb14fdd0e2745ff0a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47254", "query_cov": 1.0, "subject_cov": 0.8552036199095022, "identity": 0.997, "score": 719.0, "evalue": 2.21e-260, "valid": true, "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "ec_ids": [ "3.6.-.-" ], "uniref50_id": "UniRef50_P47254", "cog_id": "COG0486", "cog_category": "J", "go_ids": [ "GO:0002098", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0006400", "GO:0030488", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47254", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47254" ], "product": "tRNA modification GTPase MnmE" }, "genes": [ "mnmE" ], "id": "JPCNHPFPBM_58", "locus": "GCA000292505_00016" }, { "type": "cds", "sequence": "contig_1", "start": 11251, "stop": 11892, "strand": "+", "frame": 1, "gene": "tatD", "product": "putative metal-dependent hydrolase MG009", "db_xrefs": [ "COG:COG0084", "COG:N", "EC:3.1.-.-", "GO:0004536", "GO:0005829", "GO:0046872", "KEGG:K03424", "SO:0001217", "UniRef:UniRef50_P47255", "UniRef:UniRef90_P47255" ], "nt": "ATGGAATACTTTGATGCACATTGTCATTTAAATTGCGAACCTTTACTGAGTGAAATTGAAAAAAGCATCGCTAATTTCAAATTAATTAATTTAAAAGCAAATGTTGTAGGTACAGATTTGGATAACTCTAAAATTGCTGTTGAATTAGCTAAAAAATATCCTGATCTTTTAAAAGCAACCATAGGTATCCATCCAAATGATGTTCATTTAGTTGATTTTAAAAAGACAAAAAAACAACTTAATGAACTATTAATAAATAACAGAAATTTCATAAGTTGTATTGGTGAATATGGTTTTGATTATCACTACACAACAGAATTTATTGAATTGCAAAACAAATTCTTTGAGATGCAATTTGAAATAGCTGAAACTAATAAATTGGTTCACATGCTTCATATTCGTGATGCTCATGAAAAAATTTATGAAATATTAACAAGATTAAAGCCAACTCAACCTGTGATTTTTCATTGTTTCAGTCAAGATATAAATATTGCTAAAAAGCTTCTATCATTAAAAGATTTAAATATTGACATCTTCTTTTCTATCCCAGGGATAGTTACTTTTAAGAATGCTCAAGCATTACACGAAGCTTTAAAGATTATTCCTAGTGAATTACTTTTAAGTGAAACTGATTCACCGTGA", "aa": "MEYFDAHCHLNCEPLLSEIEKSIANFKLINLKANVVGTDLDNSKIAVELAKKYPDLLKATIGIHPNDVHLVDFKKTKKQLNELLINNRNFISCIGEYGFDYHYTTEFIELQNKFFEMQFEIAETNKLVHMLHIRDAHEKIYEILTRLKPTQPVIFHCFSQDINIAKKLLSLKDLNIDIFFSIPGIVTFKNAQALHEALKIIPSELLLSETDSP", "aa_hexdigest": "fe4aaaf19581833bce1f838a004b8d5f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47255", "query_cov": 1.0, "subject_cov": 0.8129770992366412, "identity": 1.0, "score": 427.0, "evalue": 1.68e-150, "valid": true, "gene": "TatD", "product": "Uncharacterized metal-dependent hydrolase MG009", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P47255", "cog_id": "COG0084", "cog_category": "N", "kegg_orthology_id": "K03424", "go_ids": [ "GO:0005829", "GO:0004536", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47255", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47255" ], "product": "Uncharacterized metal-dependent hydrolase MG009" }, "genes": [ "tatD" ], "id": "JPCNHPFPBM_59", "locus": "GCA000292505_00017" }, { "type": "cds", "sequence": "contig_1", "start": 12068, "stop": 12244, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGTTAGAACTGAAAGACTAAACGAATTGTTTTTTGTTTATCATAAAAACTTAAAAAATCAATCTAAATCTAAATATAGCAGAGCAGTTAATTACTTAAAAAGGCGTGGATTTAATTTACAAGATTTTTTAAAAGTTGGGGGTGGTGTTGGTTATTTACAAAATAAAGAATGA", "aa": "MDVRTERLNELFFVYHKNLKNQSKSKYSRAVNYLKRRGFNLQDFLKVGGGVGYLQNKE", "aa_hexdigest": "a751731d7e098c9c81701eadb454df60", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6898.821600000003, "isoelectric_point": 10.070106697082519 }, "id": "JPCNHPFPBM_60", "locus": "GCA000292505_00018" }, { "type": "cds", "sequence": "contig_1", "start": 13104, "stop": 13331, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTAAATGTTTGATCTCGTCAGCACTATTAACTAAATGTACATCTTTTCCACCGTGTGAATTACGTTTCTTAACGATGGCAGGAAATGATTTGATTGTTTCTTTACTAAGAAGAGAAGAATTGACAGTTAGAAATCTATTTTGTTTTAATCATTTATATGTTTCGTATTTATCGTTTGCTATAAAAACAACATTAAAAGGATTAACTAAAAAAGTATTTATTTGA", "aa": "MFKCLISSALLTKCTSFPPCELRFLTMAGNDLIVSLLRREELTVRNLFCFNHLYVSYLSFAIKTTLKGLTKKVFI", "aa_hexdigest": "0b51cb255ccac4ecfde7fe44c2e2d356", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8610.31359999999, "isoelectric_point": 9.505879402160645 }, "id": "JPCNHPFPBM_61", "locus": "GCA000292505_00019" }, { "type": "cds", "sequence": "contig_1", "start": 13256, "stop": 13564, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAAAATTAAATTAAAAAACAGAAAAGCATTAGTTGTTTATGACAACAAGGATGATTTTGAAAAAAATCAAACATTTGCTTTAAGTCTAATCAAGGAATTACAAAAGAAAAAACTAAATGCTGAAGTCTTATTACTTGAAAATAAAGACATTAATTTCGAAGCTAAAATTAATGAAGCTGAATTGATTTTAAATCGTTCAAGAAAAGTAGATTTTTTAAAAACCAATAATCAAATAAATACTTTTTTAGTTAATCCTTTTAATGTTGTTTTTATAGCAAACGATAAATACGAAACATATAAATGA", "aa": "MGKIKLKNRKALVVYDNKDDFEKNQTFALSLIKELQKKKLNAEVLLLENKDINFEAKINEAELILNRSRKVDFLKTNNQINTFLVNPFNVVFIANDKYETYK", "aa_hexdigest": "b8e1a2b672995e11f91a7ff849cdcef4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12009.794899999983, "isoelectric_point": 9.425422859191894 }, "id": "JPCNHPFPBM_62", "locus": "GCA000292505_00020" }, { "type": "cds", "sequence": "contig_1", "start": 13569, "stop": 14057, "strand": "-", "frame": 2, "gene": "lysX", "product": "putative protein MG012", "db_xrefs": [ "COG:COG0189", "COG:EHJQ", "GO:0005524", "GO:0005737", "GO:0009432", "GO:0018169", "GO:0036211", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P75097", "UniRef:UniRef90_P47258" ], "nt": "ATGCTCGATGTTTTTATTAATCAAATAAAACAAAGTATGGAGTTTCCTGTTATTGTTAAAAGTGTTTTTGGTTCTTTTGGTGATTATGTTTTTTTGTGTTTAGATGAACAAAAATTAAGAAAAACTTTAATGTCTTTTAATCAACAAGCAATTGTGCAAAAATACATTACTTGCTCTAAAGGTGAATCGGTAAGAGTTATTGTTGTGAACAATAAAGTTATAGGTGCTTTACATACAACTAATAATAGTGATTTTCGTTCTAATCTCAATAAAGGGGCAAAGGCAGAACGCTTTTTTTTGAATAAGGAACAAGAAAATTTAGCAGTTAAAATTAGTAAAGTAATGCAACTTTTTTATTGCGGTATTGATTTTTTGTTTGATCAAGACAGATCATTGATCTTTTGTGAAGTAAATCCTAATGTGCAATTAACAAGAAGCTCAATGTATTTAAATACTAATCTTGCAATTGAGCTTTTAAAAGCAATTTAG", "aa": "MLDVFINQIKQSMEFPVIVKSVFGSFGDYVFLCLDEQKLRKTLMSFNQQAIVQKYITCSKGESVRVIVVNNKVIGALHTTNNSDFRSNLNKGAKAERFFLNKEQENLAVKISKVMQLFYCGIDFLFDQDRSLIFCEVNPNVQLTRSSMYLNTNLAIELLKAI", "aa_hexdigest": "e7242341018933fd7eacc87b25ad2c9f", "start_type": "ATG", "rbs_motif": null, "genes": [ "lysX" ], "truncated": "3-prime", "pseudogene": { "start": 13569, "stop": 14357, "observations": { "mutation": [ 14069, 14219 ], "stop": [ 14069, 14219 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47258", "query_cov": 1.0, "subject_cov": 0.5644599303135889, "identity": 1.0, "score": 316.0, "evalue": 2.55e-107, "gene_start": 1, "gene_end": 162, "reference_start": 126, "reference_end": 287, "reference_sequence": "MKKINVVYNPAFNPISSKLNQTQLLKNASEELDIELKFFTSFDINTTKAKANLPFISNKILFMDKNIALARWLESNGFEVINSSIGINNADNKGLSHAIIAQYPFIKQIKTLLGPQNFDREWNPVMLDVFINQIKQSMEFPVIVKSVFGSFGDYVFLCLDEQKLRKTLMSFNQQAIVQKYITCSKGESVRVIVVNNKVIGALHTTNNSDFRSNLNKGAKAERFFLNKEQENLAVKISKVMQLFYCGIDFLFDQDRSLIFCEVNPNVQLTRSSMYLNTNLAIELLKAI" }, "paralog": false, "identity": 0.9923664122137404, "score": 506.0, "evalue": 7.9e-185, "description": "Internal stop codon at 14069, 14219. Nonsense mutation around 14069, 14219.", "psc": { "uniref90_id": "UniRef90_P47258", "gene": "LysX", "product": "Uncharacterized protein MG012", "uniref50_id": "UniRef50_P75097", "cog_id": "COG0189", "cog_category": "EHJQ", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0046872", "GO:0018169", "GO:0036211", "GO:0009432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47258", "UniRef:UniRef50_P75097", "COG:COG0189", "COG:EHJQ", "GO:0005737", "GO:0005524", "GO:0046872", "GO:0018169", "GO:0036211", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P75097", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75097" ], "product": "Uncharacterized protein MG012 homolog" } }, "id": "JPCNHPFPBM_63", "locus": "GCA000292505_00021" }, { "type": "cds", "sequence": "contig_1", "start": 14217, "stop": 14432, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATAAACGTTGTTTACAATCCAGCATTTAATCAAATTAGCTCTAAATTAAATCAAACTCAACTTTTAAAAAATGCTAGTGAAGAGTTAGATATAGAACTAAAATTCTTTACTAGTTTTGATATTAATACAACTAAAGCAAAAGCAAATTTACCCTTCATATCCAACAAAATTCTTTTTATGGATAAAAATATTGCTTTAGCTAGATGA", "aa": "MKKINVVYNPAFNQISSKLNQTQLLKNASEELDIELKFFTSFDINTTKAKANLPFISNKILFMDKNIALAR", "aa_hexdigest": "e05f80ee5e45e436e13d84dfe5dec6d4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8136.400799999994, "isoelectric_point": 9.631657218933103 }, "id": "JPCNHPFPBM_64", "locus": "GCA000292505_00022" }, { "type": "cds", "sequence": "contig_1", "start": 14503, "stop": 15138, "strand": "-", "frame": 1, "gene": "folD", "product": "Bifunctional protein FolD", "db_xrefs": [ "COG:COG0190", "COG:H", "GO:0000105", "GO:0004477", "GO:0004488", "GO:0005829", "GO:0006164", "GO:0009086", "GO:0035999", "SO:0001217", "UniRef:UniRef50_P47259", "UniRef:UniRef90_P47259" ], "nt": "GTGATTATTCAAGCTAATGATGATGATTCATCAGACAGTTTTATTAAACAAAAACTTATTGCTTGTAACACTGTAGGAGCAAAAAGTGAATTAATTAAACTATCTAATCAAATAACTCAAGCAGAGTTAATAGAAAAAATAATTAGTTTAAATCATGATGTAAATGTTACTGGTATCATTTTGCAATTACCAGTTTATCCACACTTAGATAAAAACTCACTACTAGAAGCAATTAATCCTTTAAAAGATGTTGATGGTTTAACAACTAATCATTTGGCTGAAATTAAACCTTGTATAGTTGAAGCTATAATAACACTAAAAGAACTATTTAACCTTGAATTTAATAATCAAAAAATTGTTGTGGTAGGTTTGGGAATAACTGGTGGCAAACCTATTTATGAATTTTTAAAAACTAGTGGTTATAAAGTTCAAGCATGCGATAAAGATACTCCAAATACATTTGAATTGATTAAAAGTGCTGATATAGTTTTTACTGCTATTGGAAAATCTCATTTTTTTCAAGCTAAAAACTTTAAAAAAGGAGTTATTTTATTTGATATAGGTGTTTCAAGAAACAAGCAAAATAAACTTTGTGGTGATATAAATCCTGAAGGCATTGAAAAAAAAGCTAGATGA", "aa": "MIIQANDDDSSDSFIKQKLIACNTVGAKSELIKLSNQITQAELIEKIISLNHDVNVTGIILQLPVYPHLDKNSLLEAINPLKDVDGLTTNHLAEIKPCIVEAIITLKELFNLEFNNQKIVVVGLGITGGKPIYEFLKTSGYKVQACDKDTPNTFELIKSADIVFTAIGKSHFFQAKNFKKGVILFDIGVSRNKQNKLCGDINPEGIEKKAR", "aa_hexdigest": "422cef830fe9d044569da2be86c1fe31", "start_type": "GTG", "rbs_motif": null, "genes": [ "folD" ], "truncated": "both", "pseudogene": { "start": 14395, "stop": 15216, "observations": { "mutation": [ 14442, 14502, 14505, 15156 ], "stop": [ 14442, 14502, 14505, 15156 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47259", "query_cov": 1.0, "subject_cov": 0.7728937728937729, "identity": 0.995, "score": 412.0, "evalue": 9.95e-145, "gene_start": 1, "gene_end": 211, "reference_start": 27, "reference_end": 237, "reference_sequence": "MSFDGKLKAQSILETYKNFDWSKCKLVIIQANDDDSSDSFIKQKLIACNTVGAKSELIKLSNQITQAELIEKIISLNHDVNVTGIILQLPVYPHLDKNSLLEAINPLKDVDGLTTNHLAEIKPCIVEAIITLKELFNLEFNNQKIVVVGLGITGGKPIYEFLKTSGYKVQACDKDTPNTFELIKSADIVFTAIGKSHFFQAKNFKKGVILFDIGVSRNKQNKLCGDINPEGIEKKARWWTKTPGGVGPFTVLAIIKNLWILHEKNKRCLQSSI" }, "paralog": false, "identity": 0.978021978021978, "score": 520.0, "evalue": 1.02e-189, "description": "Internal stop codon at 14442, 14502, 14505, 15156. Nonsense mutation around 14442, 14502, 14505, 15156.", "psc": { "uniref90_id": "UniRef90_P47259", "gene": "folD", "product": "Bifunctional protein FolD", "uniref50_id": "UniRef50_P47259", "cog_id": "COG0190", "cog_category": "H", "go_ids": [ "GO:0005829", "GO:0004477", "GO:0004488", "GO:0000105", "GO:0009086", "GO:0006164", "GO:0035999" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47259", "UniRef:UniRef50_P47259", "COG:COG0190", "COG:H", "GO:0005829", "GO:0004477", "GO:0004488", "GO:0000105", "GO:0009086", "GO:0006164", "GO:0035999" ] }, "pscc": { "uniref50_id": "UniRef50_P47259", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47259" ], "product": "Bifunctional protein FolD" } }, "id": "JPCNHPFPBM_65", "locus": "GCA000292505_00023" }, { "type": "tRNA", "sequence": "contig_1", "start": 15294, "stop": 15369, "strand": "-", "gene": "trnA", "product": "tRNA-Ala(tgc)", "amino_acid": "Ala", "anti_codon": "tgc", "score": 68.8, "nt": "GGGGATGTAGCTCAACTGATAGAGCACCTGATTTGCACTCAGGAGGTTGAGGGTTTGAGACCCTTCATCTCCACCA", "db_xrefs": [ "SO:0000254" ], "anti_codon_pos": [ 15334, 15336 ], "id": "JPCNHPFPBM_36", "locus": "GCA000292505_00024" }, { "type": "tRNA", "sequence": "contig_1", "start": 15375, "stop": 15451, "strand": "-", "gene": "trnI", "product": "tRNA-Ile(gat)", "amino_acid": "Ile", "anti_codon": "gat", "score": 84.3, "nt": "GGAAGTATAGCTCAGTTGGTTAGAGCACACCCCTGATAAGGGTGAGGTCGATGGTTCAAGTCCATTTACTTCCACCA", "db_xrefs": [ "SO:0000263" ], "anti_codon_pos": [ 15415, 15417 ], "id": "JPCNHPFPBM_35", "locus": "GCA000292505_00025" }, { "type": "cds", "sequence": "contig_1", "start": 15555, "stop": 16310, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B41887", "UniRef:UniRef90_UPI0001B41887" ], "nt": "ATGGGGCTTGTTTTAAAAGAATTTAACAATAAAATAAGAACAGCATTAATTCTCGCTCCTTTTTTCACTTTCGCTCAGATAGTGATTGATTTAATCATTCCTTCTTTTTTAGCAAGTGCTATTTCAGTTGTGTTTTCAATTGATAAATTAAAACAGGATGAGTCAGGCGGAAAGACAATTTCAGTTGATTTTATTGGTGGGGCTAATATTAATTTTGCTAATGTAAGAGAAGCGCAAATAGTTTTAGCAACCACAGTTATTTTACTTGCATTATGTGGACTTTTTTTTGGTCTAATATCTATTTATTGTGCAAGTTATGTTTCAGCAAACACTTCTTATCTATTAAGAAAAAAGATCTTTGCAAAATTAATGCGAATCACAACCCCATCACATGACCATTATGGTTCTTCTACTTTATTAGTTAGATTAACAAATGATGTTTATTTGATGGAGGTAATTGCTTTTGATTTTTTAAGGCTAATTATTCGTGCTCCGCTTTTGTTTATAGGTGGATTAGTGTTTGCAGTAACTACAAATCAAGATATGTCAATATCTTTACTAATTACTTTTCCTTTGATTCTTTTGGTAATTGGTATTCTAAACCGTAAATCTATTCCTTTATTTAAAGAAAATCAAAAATCAGTTGATAAGATAAATGAAAGAGTAGAAGAAGATGTTTCAGGTTATAAAGTAATTCAATCTTTTAATCTTCATTCCTTTACAAATAACAAATTTAAGATTGCAAATGAAGGATGA", "aa": "MGLVLKEFNNKIRTALILAPFFTFAQIVIDLIIPSFLASAISVVFSIDKLKQDESGGKTISVDFIGGANINFANVREAQIVLATTVILLALCGLFFGLISIYCASYVSANTSYLLRKKIFAKLMRITTPSHDHYGSSTLLVRLTNDVYLMEVIAFDFLRLIIRAPLLFIGGLVFAVTTNQDMSISLLITFPLILLVIGILNRKSIPLFKENQKSVDKINERVEEDVSGYKVIQSFNLHSFTNNKFKIANEG", "aa_hexdigest": "e3d948e9cf5764e3c0f6c60d551a7700", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 15555, "stop": 16613, "observations": { "mutation": [ 16308 ], "stop": [ 16308 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001B41887", "query_cov": 1.0, "subject_cov": 0.6519480519480519, "identity": 0.996, "score": 461.0, "evalue": 1.22e-161, "gene_start": 1, "gene_end": 251, "reference_start": 1, "reference_end": 251, "reference_sequence": "MGLVLKEFNNKIRTALILAPFFTFAQIVIDLIIPSFLASAISVVFSIDKLKQDESGGKTISVDFIGGANINFANVREAQIVLATTVILLALCGLFFGLISIYCASYVSANTSFLLRKKIFAKLMRITTPSHDHYGSSTLLVRLTNDVYLMEVIAFDFLRLIIRAPLLFIGGLVFAVTTNQDMSISLLITFPLILLVIGILNRKSIPLFKENQKSVDKINERVEEDVSGYKVIQSFNLHSFTNNKFKIANEGWKKNSTSSLFINSLNIPFTFFLSSLTIIIALLLVFQLDSSVSVDPLPQDAAIRPNIFAFFQYNFYIVLGFILTSLTMVNFNRSRVALGRIKDILSQPEIKTITNKDQKELLPTLEFRNISFGLGNKNNNNFFTK" }, "paralog": false, "identity": 0.9914772727272727, "score": 663.0, "evalue": 1.44e-243, "description": "Internal stop codon at 16308. Nonsense mutation around 16308.", "psc": { "uniref90_id": "UniRef90_UPI0001B41887", "product": "ABC transporter permease", "uniref50_id": "UniRef50_UPI0001B41887", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001B41887", "UniRef:UniRef50_UPI0001B41887" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001B41887", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B41887" ], "product": "ABC transporter permease" } }, "id": "JPCNHPFPBM_66", "locus": "GCA000292505_00026" }, { "type": "cds", "sequence": "contig_1", "start": 16536, "stop": 16967, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGGTGAACTTTAATCGTTCTAGAGTTGCACTAGGCAGAATTAAAGATATTTTATCTCAGCCTGAAATAAAAACTATTACAAATAAAGATCAGAAAGAATTATTACCTACCTTGGAGTTTAGAAATATCTCTTTTGGTCTTGGCAATAAGAACAATAACAATTTTTTACAAAATCTTAGTTTTAAATTTGAAGCATATAAAACATATGGTATTGTTGGGCCTACAGGCTCTGGAAAATCTTTAATTGCTAACATTATCAGTGGTTTATATGAGCCTAATGAGGGTGAAATTCTCATTGGTGGGGAGAAAATTCAATCTATTGACAGTTTATATCTATCAGAAATGATAGGAATTGTTTTCCAACAAAACATCCTTTTTAAAGGAACAATTTCTTCTAATATAAAAATTGGTATTGAAACTAGAAGTGATTGA", "aa": "MVNFNRSRVALGRIKDILSQPEIKTITNKDQKELLPTLEFRNISFGLGNKNNNNFLQNLSFKFEAYKTYGIVGPTGSGKSLIANIISGLYEPNEGEILIGGEKIQSIDSLYLSEMIGIVFQQNILFKGTISSNIKIGIETRSD", "aa_hexdigest": "2c169efe25b694f9012db363bbf0cab0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 76, "aa_cov": 0.5244755244755245, "hmm_cov": 1.0, "evalue": 5.8452728863060654e-18, "score": 60.9252815246582, "start": 56, "stop": 130 } ], "seq_stats": { "molecular_weight": 15903.042799999994, "isoelectric_point": 8.858745765686034 }, "id": "JPCNHPFPBM_67", "locus": "GCA000292505_00027" }, { "type": "cds", "sequence": "contig_1", "start": 17001, "stop": 17426, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DF8197" ], "nt": "ATGAAAAACGCAGCTAAAATAGCTTGCGCTGACACCTTTATTGAAAAGTTTTCTGATAGTTATGATCACAATGTTGAACAGTTAGGTAAAAACTTATCTGGTGGACAAAAACAAAGAGTTGCTATTGCAAGAACTTTAATTACAAAACCAAGAATTTTAGTTTTTGATGATAGTATGAGTGCTCTTGATGCTCTAACTGAAAAAAAAGTAAGAGAAAATATTGAAAATGATTTAAAGCTAACTACCAAAATCATTATTAGTCAAAACATTAATTCAATTAAACACGCAGATAAAATTTTGGTAATTGATAATGGCAGAATTGTTGGTTTTGATAGTGATCAAAAACTAATGAAAAATTGTTCACTTTATCAAAAGATGAAAGAATCTCAAAAAGATTTGGGAGGTGATTTTGATGCAGTTAACTAG", "aa": "MKNAAKIACADTFIEKFSDSYDHNVEQLGKNLSGGQKQRVAIARTLITKPRILVFDDSMSALDALTEKKVRENIENDLKLTTKIIISQNINSIKHADKILVIDNGRIVGFDSDQKLMKNCSLYQKMKESQKDLGGDFDAVN", "aa_hexdigest": "4b00981fe19cc59148815d4e1c1e9d57", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021DF8197", "query_cov": 0.9219858156028369, "subject_cov": 0.8441558441558441, "identity": 0.546, "score": 140.0, "evalue": 3.06e-40, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI0021DF8197" }, "pscc": { "uniref50_id": "UniRef50_UPI0021DF8197", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DF8197" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "JPCNHPFPBM_68", "locus": "GCA000292505_00028" }, { "type": "cds", "sequence": "contig_1", "start": 17512, "stop": 17784, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTGTATAGTTCTTGGTATTTTATATGGAATTGCTAACCCTATCTTATTAGCACAAGGTCTTGGTTTTATTTTTCCTATTACTAATAGTAATGGTCGTGCTGTTGACTCAATATATTCATTAATTTACCCAACAAATTTAAATGTATTCATTAGGCTCACAATTGTGAGCGTAACTGTTTTTATAGCTTATGCATTAATCTTTGTATTTAATGTAGCGCAAAACTATGTAGGGATTAAACTTTACCAGCAAACATGTGCTACTTTGCGTTGA", "aa": "MCIVLGILYGIANPILLAQGLGFIFPITNSNGRAVDSIYSLIYPTNLNVFIRLTIVSVTVFIAYALIFVFNVAQNYVGIKLYQQTCATLR", "aa_hexdigest": "a004c9998b828b22d5e5636d2eee90a4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9934.723599999994, "isoelectric_point": 9.137958335876466 }, "id": "JPCNHPFPBM_69", "locus": "GCA000292505_00029" }, { "type": "cds", "sequence": "contig_1", "start": 17800, "stop": 18414, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGAGTATGTCAACCAGCTTTTTTGATACGCAAAATAATGGTGATCTTATGAGTAGGTTAACTAATGATATGTATAACATTGATAACCTATTCACTCAAGCTGGTGGACAAGCTATTCAAAGTTTGTTTAATATTTTAACAACCTCAGTATTAATATTTTTATTAAGCCCAGTTATTGCACTTATTTCACTTTCAATTTTAGCTACATTAATTACTTTTTCTTTTGCCTTTCTAAAGAAATCAAAAACTTCATATAGTCAAGTACAAAATAATTTGGGTGATATGTCTGGTTATATTGAAGAGGTTTTAACTAATCATAAGGTTGTTCATGTCTTGAAGTTGCAAGAGATAATGATTAAGGATTTTGATCAATACAACAAATCAATGATCAAACCAACTGTAAGAGGGAATACATATTCGATCTTTCTTTTTTCTTGGTTTGGTTTTATATCAAATATTACTTATCTGGTTTCTATATCAATTGCTACTGCTTTTAGTGTTAATTCTATTCCTTCATTTGGAATTAGTGTTATTAACTATTCATTCATGTTGTCTTACATTGCTTCTTTAAGACAAATAACTTTAGCATTAGATCAAATCTTTACCCTTTGA", "aa": "MQSMSTSFFDTQNNGDLMSRLTNDMYNIDNLFTQAGGQAIQSLFNILTTSVLIFLLSPVIALISLSILATLITFSFAFLKKSKTSYSQVQNNLGDMSGYIEEVLTNHKVVHVLKLQEIMIKDFDQYNKSMIKPTVRGNTYSIFLFSWFGFISNITYLVSISIATAFSVNSIPSFGISVINYSFMLSYIASLRQITLALDQIFTL", "aa_hexdigest": "8faf67753c8466a8959003904e3ab122", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22909.30110000001, "isoelectric_point": 6.7110593795776365 }, "id": "JPCNHPFPBM_70", "locus": "GCA000292505_00030" }, { "type": "cds", "sequence": "contig_1", "start": 18886, "stop": 19242, "strand": "+", "frame": 1, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X7NT64" ], "nt": "ATGCGTTTACCAAAAGGATATGACACTTATATTTCCAATAAAGCAGATTATCTTTCTGTTGGTGAAAGGCAATTATTAACAATTGCCAGAGCAGTAATCCGTAATGCTCCAGTTTTGCTCTTAGATGAAGCAACTAGTTCAGTTGATGTCCATTCAGAAAAATTAATTCAAGAATCAATAGGAAGGTTAATGAAAAATAAAACTTCTTTTATAATTTCTCATCGTCTTTCAATTATTCGTGATGCAACATTAATAATGGTTATTAATGATGGTAAAGTACTTGAAATGGGTAATCAGAATCAGCTGATGAAACAAAATGGATTTTATGCACGTTTAAAACAATCTTCGGTTCGTTAA", "aa": "MRLPKGYDTYISNKADYLSVGERQLLTIARAVIRNAPVLLLDEATSSVDVHSEKLIQESIGRLMKNKTSFIISHRLSIIRDATLIMVINDGKVLEMGNQNQLMKQNGFYARLKQSSVR", "aa_hexdigest": "6f964d89aca3c5f5b8563aecce1ebada", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00131ED865", "query_cov": 0.9576271186440678, "subject_cov": 0.9338842975206612, "identity": 0.5579999999999999, "score": 131.0, "evalue": 2.08e-37, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_A0A7X7NT64" }, "pscc": { "uniref50_id": "UniRef50_A0A7X7NT64", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X7NT64" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "JPCNHPFPBM_71", "locus": "GCA000292505_00031" }, { "type": "cds", "sequence": "contig_1", "start": 19252, "stop": 19623, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04434.22" ], "nt": "ATGACTGTTGCTGAAATTAAAAAACTTGCATTAAATAATCAGGTATTTAATGAAGCAAAAGCGCTTTTAGAAAAAGGTAATGTTATTTTTCCAAAAAAATTCTTAAAGCGAAAGAAGATCATTATTGAAGTATTAGATGGTAAGGTTTTTAAGGTTCAAATTAATTTAAAAACCGCTGCTGCACACTTGGATTGTAGTTGCTCTAATGATAAGCAAAATTGCGTTCATATAATTGCAGCGCTTTTGAAATATAATGATCTAAAAAATCAAGATAACAAAGAATTTGACCTTAATAAAGCCGATAAATTAGAGTGCAAAGAAGTTGAAATTCTAATTGAAAATGTTAGCTTAGCAATTGTTAATGGCAGCTGA", "aa": "MTVAEIKKLALNNQVFNEAKALLEKGNVIFPKKFLKRKKIIIEVLDGKVFKVQINLKTAAAHLDCSCSNDKQNCVHIIAALLKYNDLKNQDNKEFDLNKADKLECKEVEILIENVSLAIVNGS", "aa_hexdigest": "91ce7bc8403e9eef42379ea6dc1b9554", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04434.22", "name": "SWIM", "length": 39, "aa_cov": 0.2926829268292683, "hmm_cov": 0.9743589743589743, "evalue": 1.1962164001607368e-08, "score": 29.489242553710938, "start": 50, "stop": 85 } ], "seq_stats": { "molecular_weight": 13824.102099999984, "isoelectric_point": 8.85700511932373 }, "id": "JPCNHPFPBM_72", "locus": "GCA000292505_00032" }, { "type": "cds", "sequence": "contig_1", "start": 19825, "stop": 20736, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATATGAAAATAACAGTTTGTTTTTTAATTTAGATCAATTCCTAAGCCTAGTTAAGGAAGTTAAAAAACCTTCATTATTCTTATTAAATGAGGATAAAACTGACAATATTCTTTTCTTAAGAAGTCAACATAAAATCAATGGATTAAGCCACGTTTGTGGTTTTTTAAATAATAAGGTTTTTGATTTTGTATCCTACAATGAAAAAACTAAGCAAATTGTTTTACGCTTAGCTTATCTTAATAAGTTCACTGATTTTAAATTTCCATACAACATTAACATCTATAAACTTGCTTTTGGAGAAACATTATTTTTCCATTTTTTAATCCATTTAAAAATGAATGGTTTTAAAAACATCTTTTTTCAAAGTGATGTTGCTATTGTTAAGGAAAGTGAATATCTACCTAAGATGTTTTTGACTATTGAATTTAATACACAAAAAAACAAGTTTATAACTGATGCTTTTTTTAAATATAAGAATAAAAATAGCAATACTTTAACAACTGTTTACCCCCATCGCTATTACTTAGCTCAAAAAACTAATACATCAAACTTCAACCGTTTACTTTTTTATGAACAAGCACTACAAAGGTTTTATGAAGAATTATTTCAAATAGATTATTTAAGAAGATTTGAAAACATTCCCATTAAAGATAAGAATCAAATTGCGCTTTTTAAAACTGTTTTTGATGATTACAAAACCATTGATTTAGCAGAATTGAAACTTACAAGTAATCTCTTAAATTACAAACAGTTACATTTTTCAATAAGTGATATTAAAGCTTTAAAAATTGAAGATAGACAACTAAAAATTGAATTTAAAGCTGGCGGGATAGATCTAAAGTTAATTAAAAGTGTTTTAAGTAACTACTATAAGGGTAATGCTATTTGTATTGGTGAAGATGGTTGA", "aa": "MQYENNSLFFNLDQFLSLVKEVKKPSLFLLNEDKTDNILFLRSQHKINGLSHVCGFLNNKVFDFVSYNEKTKQIVLRLAYLNKFTDFKFPYNINIYKLAFGETLFFHFLIHLKMNGFKNIFFQSDVAIVKESEYLPKMFLTIEFNTQKNKFITDAFFKYKNKNSNTLTTVYPHRYYLAQKTNTSNFNRLLFYEQALQRFYEELFQIDYLRRFENIPIKDKNQIALFKTVFDDYKTIDLAELKLTSNLLNYKQLHFSISDIKALKIEDRQLKIEFKAGGIDLKLIKSVLSNYYKGNAICIGEDG", "aa_hexdigest": "0fcf0b774d610e6fe8b99e46423bfafd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 36009.174299999984, "isoelectric_point": 9.250906944274899 }, "id": "JPCNHPFPBM_73", "locus": "GCA000292505_00033" }, { "type": "cds", "sequence": "contig_1", "start": 20743, "stop": 21177, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00176.29" ], "nt": "TTGAACGATGAAAATGCTAAAGCACTAATTTCATTTTGGAGTCAAATTGACTTAAGAAATGCTACTTGTGATGCTAATAACAATTTGCTTCTTGCTAAATACCACTTGTTTGAAGTTGTAGATACTATTAGTAAATACACTGATGTAACTAATTTATTAGATGAAAAAACAGCCTTACAATTAAAAATAGCTAGTGAAAATCAATTTCATCTTTCGTTAGATAATAACCAAATTAATAACTTACGTAAATATCAAAAAGAAGGAGTTAAATGGATAAGGGCATTAGAAGATAATCAGTTTGGTGGAATTCTAGCAGATGAAATGGGGTTAGGTAAAACTGCTCAAGTGATCTTTGCAATGCTGGATAGTTATCAATCAACAAAATCACTTTTACCTAGTTTAATTATCGTTCCAGCATCCTTACTCTTAAATTGA", "aa": "MNDENAKALISFWSQIDLRNATCDANNNLLLAKYHLFEVVDTISKYTDVTNLLDEKTALQLKIASENQFHLSLDNNQINNLRKYQKEGVKWIRALEDNQFGGILADEMGLGKTAQVIFAMLDSYQSTKSLLPSLIIVPASLLLN", "aa_hexdigest": "2f1ca9220bc4611b7188c1caec9439d5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00176.29", "name": "SNF2-rel_dom", "length": 63, "aa_cov": 0.4097222222222222, "hmm_cov": 1.0, "evalue": 4.311840414284417e-17, "score": 57.18943405151367, "start": 84, "stop": 142 } ], "seq_stats": { "molecular_weight": 16217.3373, "isoelectric_point": 5.07500591278076 }, "id": "JPCNHPFPBM_74", "locus": "GCA000292505_00034" }, { "type": "cds", "sequence": "contig_1", "start": 21208, "stop": 21321, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAATAGTTACTGCTAATGGCAATTTTAAAGAACGCTCACAGATATATGAATCATTAAAAAATCAGATTTTGTTAATGAGTTTTAATGTCTTGAGAAGTGATATTAAATGA", "aa": "MKIVTANGNFKERSQIYESLKNQILLMSFNVLRSDIK", "aa_hexdigest": "592308773786ba569c9f8e82a67aa7da", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4330.0380000000005, "isoelectric_point": 9.819711494445798 }, "id": "JPCNHPFPBM_75", "locus": "GCA000292505_00035" }, { "type": "cds", "sequence": "contig_1", "start": 21577, "stop": 22128, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00271.37", "PFAM:PF00176.29" ], "nt": "ATGAAAAAAACAAGTCCTTTTATTTTAAGAAGGACTAAAAATAAAGTTTTAAAAGAACTACCTAAAAAAATTATTACTGATATCTATGTTGAACTTAGTGAAGAACATCAAAAACTGTATGATAAACAAAAAACAGATGGTTTGAAGGAGATTAAAGAAAGTGATGCTAAAAATGCTTTAAATATCCTTAGTTTAATCTTGAAATTAAGGCATATTTGTAGCTTAGTTAAAGATAATGATGTTAATGATTTTGAAGATAATTCCAAAGCTAATACAGCTTTAAACATTATTTATGAAGCACTTGAAAATAAACGCAAAGTTATTTTGTTTACTCAATTTTTAGATGTAATTGATTGTTTTAAGCAAACTTTAAAAAATCAAAAGATTGATCACCTGGTATTTGATGGTAGAAAAACTGTGAAGAATAGAAACACTATTATCCAGAAGTTCAATAGTGCTAAAGAACCTTGTGTGATGTTAGCTTCTTTAAAAGCTGGTGGAGTTGGTATTAACTTAACTGCTGCTGAAGTTGTTATCCATTTTGATGTGTGA", "aa": "MKKTSPFILRRTKNKVLKELPKKIITDIYVELSEEHQKLYDKQKTDGLKEIKESDAKNALNILSLILKLRHICSLVKDNDVNDFEDNSKANTALNIIYEALENKRKVILFTQFLDVIDCFKQTLKNQKIDHLVFDGRKTVKNRNTIIQKFNSAKEPCVMLASLKAGGVGINLTAAEVVIHFDV", "aa_hexdigest": "44ddcc21a6054c058f1ee66f9ef7d0b3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00271.37", "name": "Helicase_C", "length": 91, "aa_cov": 0.4808743169398907, "hmm_cov": 0.967032967032967, "evalue": 7.4174614312531355e-16, "score": 53.53043746948242, "start": 95, "stop": 182 }, { "id": "PF00176.29", "name": "SNF2-rel_dom", "length": 74, "aa_cov": 0.3879781420765027, "hmm_cov": 1.0, "evalue": 2.9943292680148815e-11, "score": 38.016639709472656, "start": 4, "stop": 74 } ], "seq_stats": { "molecular_weight": 20941.229300000003, "isoelectric_point": 9.315826606750488 }, "id": "JPCNHPFPBM_76", "locus": "GCA000292505_00036" }, { "type": "cds", "sequence": "contig_1", "start": 22389, "stop": 23558, "strand": "+", "frame": 3, "gene": "dnaJ", "product": "molecular chaperone DnaJ", "db_xrefs": [ "COG:COG0484", "COG:O", "EC:1.8.4.-", "GO:0005515", "GO:0005524", "GO:0005737", "GO:0006260", "GO:0006457", "GO:0008270", "GO:0009408", "GO:0031072", "GO:0042026", "GO:0051082", "GO:0051085", "RefSeq:WP_014894099.1", "SO:0001217", "UniParc:UPI00027B3189", "UniRef:UniRef100_UPI00027B3189", "UniRef:UniRef50_P78004", "UniRef:UniRef90_P47265" ], "nt": "ATGGCTGCTGGTAAAAGGGATTATTATGAAGTTCTAGGGATATCTAAAAACGCTAGTTCTCAAGACATAAAAAGAGCTTTTAGAAAGCTTGCAATGCAATATCACCCCGATCGTCATAAAGCAGAAAATGAAACTACTCAAAAACAAAATGAGGAAAAGTTTAAAGAGGTTAATGAAGCATACGAAGTTCTAAGTGATGAAGAAAAACGTAAGCTTTATGACCAGTTTGGTCATGAAGGGTTAAATGCTTCTGGTTTTCATGAAGCAGGGTTTAATCCTTTTGACATCTTTAATAGTGTTTTTGGTGAGGGATTTTCCTTTGGAATGGATGGTGATTCACCATTTGATTTCATTTTTAATCGTTCTAAAAAACGTCAACAACAAATTGTTGTTCCCTATAACCTTGATATTGCCTTAGTAATTGAAATTAACTTTTTTGAAATGACTAACGGTTGCAACAAAACCATCGAATATGAAAGAAAAGTTTCATGTCATAGTTGTAATGGTTTTGGCGCTGAAGGTGGGGAAAGCGGATTGGATCTTTGTAAGGATTGTAATGGCAATGGTTTTGTTATTAAAAACCAACGTTCTATCTTTGGAACCATTCAATCCCAAGTGTTGTGTTCAACTTGCAATGGACAAGGAAAACAAATTAAAGTTAAGTGCAAAACTTGTCGTTCTAACAAATACACTGTCACCAATCAAATTAAAGAGATTAATATTCCAGCAGGAATGTATAGTGGTGAAGCTTTAGTTGATGAAAGTGGTGGTAATGAATTTAAAGGTCACTATGGAAAATTAATCATTCAAGTGAATGTATTGGCAAGTAAGATTTTCAAACGTAGTGATAATAATGTTATTGCCAATGTTTTAGTAGATCCAATGGTTGCTATAGTTGGTGGGGTAATTGAACTACCTACTCTTGAAGGGATTAAAGAATTTAATATTAGACCAGGCACTAAGAGTGGCGAACAAATTGTTATTCCTAACGGTGGGATTAAATTCTCAAAGAGTTTTAAAAGAAAAGCTGGGGACTTAATCATTATTATTAGTTATGCACGTCCTTGTGAATACACTAACTTAGAATTGAAAAAATTACGTGAGTTTATCAAACCTAATCAAGAGGTTAGACAATACTTAAATACTTTAAAAAATGAATACAAAACTTAA", "aa": "MAAGKRDYYEVLGISKNASSQDIKRAFRKLAMQYHPDRHKAENETTQKQNEEKFKEVNEAYEVLSDEEKRKLYDQFGHEGLNASGFHEAGFNPFDIFNSVFGEGFSFGMDGDSPFDFIFNRSKKRQQQIVVPYNLDIALVIEINFFEMTNGCNKTIEYERKVSCHSCNGFGAEGGESGLDLCKDCNGNGFVIKNQRSIFGTIQSQVLCSTCNGQGKQIKVKCKTCRSNKYTVTNQIKEINIPAGMYSGEALVDESGGNEFKGHYGKLIIQVNVLASKIFKRSDNNVIANVLVDPMVAIVGGVIELPTLEGIKEFNIRPGTKSGEQIVIPNGGIKFSKSFKRKAGDLIIIISYARPCEYTNLELKKLREFIKPNQEVRQYLNTLKNEYKT", "aa_hexdigest": "3eec74deae4441d3aa5c8f9b2719470b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3189", "ncbi_nrp_id": "WP_014894099.1", "uniref100_id": "UniRef100_UPI00027B3189", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3189", "RefSeq:WP_014894099.1", "UniRef:UniRef100_UPI00027B3189" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3189", "uniref90_id": "UniRef90_P47265" }, "psc": { "uniref90_id": "UniRef90_P47265", "gene": "dnaJ", "product": "molecular chaperone DnaJ", "ec_ids": [ "1.8.4.-" ], "uniref50_id": "UniRef50_P78004", "cog_id": "COG0484", "cog_category": "O", "go_ids": [ "GO:0005515", "GO:0005524", "GO:0005737", "GO:0006260", "GO:0006457", "GO:0008270", "GO:0009408", "GO:0031072", "GO:0042026", "GO:0051082", "GO:0051085" ] }, "pscc": { "uniref50_id": "UniRef50_P78004", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78004" ], "product": "Chaperone protein DnaJ" }, "genes": [ "dnaJ" ], "id": "JPCNHPFPBM_77", "locus": "GCA000292505_00037" }, { "type": "cds", "sequence": "contig_1", "start": 23638, "stop": 23838, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATATCCATGGCGGACCTGGTTCTGGTACTGATGAAGGATGTTTAAAGTATTTTGATCTTGAAACAACTTGGATTATTTTATTAGATCAAAGAGGTTGTGGTAAGAGCAAGACTAATGATATCTTTTATGAAAATAACACTGATAAATTAGTTAGTGATTTTGAAATTTTACGTCAAAAATTAAACATTAAAAGCTGA", "aa": "MYIHGGPGSGTDEGCLKYFDLETTWIILLDQRGCGKSKTNDIFYENNTDKLVSDFEILRQKLNIKS", "aa_hexdigest": "f2ac95f2749caeac5efb7135488bbbb7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7531.424499999998, "isoelectric_point": 5.14542942047119 }, "id": "JPCNHPFPBM_78", "locus": "GCA000292505_00038" }, { "type": "cds", "sequence": "contig_1", "start": 23968, "stop": 24135, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGATTAGGAAAAATGTTTTATCCTTATGAACACCAACGCTTTATGGATAGTATCCCTAAAGCTTATCAGAACAGTTATGAACAAATTGTTAACTACTGTTATGATCAATTTCAAAATGGTGATGAATCAACCAAAGAAAAACTTGCTAAAGCTTGGGTGGATTGA", "aa": "MGLGKMFYPYEHQRFMDSIPKAYQNSYEQIVNYCYDQFQNGDESTKEKLAKAWVD", "aa_hexdigest": "4fa629ae8e0979194b861e4c5b6ce087", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6607.3088000000025, "isoelectric_point": 5.156797218322752 }, "id": "JPCNHPFPBM_79", "locus": "GCA000292505_00039" }, { "type": "cds", "sequence": "contig_1", "start": 24214, "stop": 24468, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATGTCACTATGCAGTTAATAAAAGTTTTTTGGATGAAAACTTCATTCTAGATAACATTAGTGTTCTTAAAAATAAAAGTATTTATTTGGCTCATGGTAGATTTGATTTGATCTGTCCTTTATATCAACCATTAGCATTAAAACAAGCATTCCCTGAATTACAACTTTATGTAACCAATAATGCTGGTCATAGTGGTAGTGATGCTAATAATTTAGCAACTATAAAACACCTTTTAAAAACTTACCTTTAA", "aa": "MECHYAVNKSFLDENFILDNISVLKNKSIYLAHGRFDLICPLYQPLALKQAFPELQLYVTNNAGHSGSDANNLATIKHLLKTYL", "aa_hexdigest": "b972969db37f342178fedff9182e500c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9524.865399999991, "isoelectric_point": 6.8863508224487315 }, "id": "JPCNHPFPBM_80", "locus": "GCA000292505_00040" }, { "type": "cds", "sequence": "contig_1", "start": 24468, "stop": 24710, "strand": "+", "frame": 3, "gene": null, "product": "Methionyl/Leucyl tRNA synthetase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A093YS71" ], "nt": "ATGAAGCGTTGTTATATTACAACCCCTATCTACTACGCATCAGGTAAGCCCCATATAGGTCATGCTTTTACCACTATTTTGGCGGATGTAATTAAGCGTTTTAAAATCCAAAACGGATATGAGGCTTTTTTGCTTGTTGGCAGTGATGAACATGGCAATAAAATAGAAAGTAAAGCTAAAAGCTTAAATTTAGATCCTAAAACATTTGTTGATATTAACGCTCAAGCTTTTAAGTTAATGTGA", "aa": "MKRCYITTPIYYASGKPHIGHAFTTILADVIKRFKIQNGYEAFLLVGSDEHGNKIESKAKSLNLDPKTFVDINAQAFKLM", "aa_hexdigest": "648efa85a2d31d25aea590fb5cab23b8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A093YS71", "query_cov": 0.95, "subject_cov": 0.9743589743589743, "identity": 0.513, "score": 82.0, "evalue": 5.85e-19, "valid": false, "product": "Methionyl/Leucyl tRNA synthetase domain-containing protein", "uniref50_id": "UniRef50_A0A093YS71" }, "pscc": { "uniref50_id": "UniRef50_A0A093YS71", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A093YS71" ], "product": "Methionyl/Leucyl tRNA synthetase domain-containing protein" }, "genes": [], "id": "JPCNHPFPBM_81", "locus": "GCA000292505_00041" }, { "type": "cds", "sequence": "contig_1", "start": 24978, "stop": 25094, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAGGCAAAAATCAACTTAACATTGTTCCTAAATCACGTGCTTCTGAATTAAAAAATAACTTTATAAACAATGGTTTAAACGATTTAGCATTAACAAGAAAAAATGTTACTTGA", "aa": "MIGKNQLNIVPKSRASELKNNFINNGLNDLALTRKNVT", "aa_hexdigest": "1b4e1135fda699dc66bbaaf0e280ba4a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4239.855900000001, "isoelectric_point": 10.446408653259276 }, "id": "JPCNHPFPBM_82", "locus": "GCA000292505_00042" }, { "type": "cds", "sequence": "contig_1", "start": 25371, "stop": 25766, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAAATCTTTAAACAACGTTATCTCTCCAGAACAATTAATTGATCAATTTGGTGTTGATGGTACAAGATATTGTTTATTAAAAGAGATGCGTTTAGATAAAGATAATCGTTGTAGTATTAGCATCTTAAAAGAGATTTATAATGCTGATCTTGCCAATAGTTTTGGAAACCATGTTTCACGTACTTTTGGCATGATTAAAAAGTATCTAAACGGCAAATTAGAATACCAAATTATTACTGATAATGCACTTCAAAAAATAATGATTTTAATAGATGAATCAATCGTTAAATTTGATCATTACTTTAACAGTTATGAATTTTATAGAGCGATTAATCTACTTTTAAAAATTGTTTTTGAATTAAGTAAATTAATTGATGATTTCAAACCATGA", "aa": "MSKSLNNVISPEQLIDQFGVDGTRYCLLKEMRLDKDNRCSISILKEIYNADLANSFGNHVSRTFGMIKKYLNGKLEYQIITDNALQKIMILIDESIVKFDHYFNSYEFYRAINLLLKIVFELSKLIDDFKP", "aa_hexdigest": "701ac514c06f70728258b7f3e525a70d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15311.601199999988, "isoelectric_point": 6.7220861434936525 }, "id": "JPCNHPFPBM_83", "locus": "GCA000292505_00043" }, { "type": "cds", "sequence": "contig_1", "start": 25770, "stop": 26006, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAAAAATCAGGAATTCTCACTTTTAAAACAACTACTTTTTACTTGTGTTAGGTGTGTGCAGGTATGCTATGTGTTGTTAACACCTATCTTAGTAAATACTGCTTCAAAAGTTTTTCATTTATTTAATTTCGCTGATGATGCCTGTAGAAAAGACCAATTAAGAGATGCAACTTTATTAAAAAAAATTATTATCTCTAATTCAATGGAAGTTTTATTTAAAAGAGTAGATTAA", "aa": "MFKNQEFSLLKQLLFTCVRCVQVCYVLLTPILVNTASKVFHLFNFADDACRKDQLRDATLLKKIIISNSMEVLFKRVD", "aa_hexdigest": "6f5655566eed17e86cdd8dfc17e96edd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9085.770299999996, "isoelectric_point": 9.070653343200682 }, "id": "JPCNHPFPBM_84", "locus": "GCA000292505_00044" }, { "type": "cds", "sequence": "contig_1", "start": 26036, "stop": 26128, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATTAGAGTACCTCAATCTTATTTCTCAAGCTAAAGTTATTGCAGAAAAACAATTTAAAGCTAACCCTTTTTCTTTTGAAACAATTTGA", "aa": "MQLEYLNLISQAKVIAEKQFKANPFSFETI", "aa_hexdigest": "dc9fa5bbd9bd6161886b243b351fd3a1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3502.0416000000005, "isoelectric_point": 5.985566520690917 }, "id": "JPCNHPFPBM_85", "locus": "GCA000292505_00045" }, { "type": "cds", "sequence": "contig_1", "start": 26261, "stop": 26473, "strand": "+", "frame": 2, "gene": "rpoE", "product": "DNA-directed RNA polymerase subunit delta", "db_xrefs": [ "COG:COG3343", "COG:K", "EC:2.7.7.6", "GO:0000428", "GO:0003899", "GO:0006351", "GO:0006355", "KEGG:K03048", "SO:0001217", "UniRef:UniRef50_P47268", "UniRef:UniRef90_P47268" ], "nt": "ATGAAGTTTGATGAATGGAACAAGATATCACAATCTATGTTTGTTACAAAGGAGATTTTTGAAGAAGGTTATGAAGATCTTTCCAATAAAAAAGTAGAACCTGAGGAAGGAGTTGGTGATTTCATTATGGGAAATGATGGTGATGACAATGAAACTGGCAGTGAAATAGTACAAGGTTTAATTAATGATTCATTCAGTGAGGAAAATCAATAG", "aa": "MKFDEWNKISQSMFVTKEIFEEGYEDLSNKKVEPEEGVGDFIMGNDGDDNETGSEIVQGLINDSFSEENQ", "aa_hexdigest": "33251ba9ccc549576565c89ea720e8dd", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpoE" ], "truncated": "5-prime", "pseudogene": { "start": 26036, "stop": 26473, "observations": { "mutation": [ 26126, 26243 ], "stop": [ 26126, 26243 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47268", "query_cov": 1.0, "subject_cov": 0.4827586206896552, "identity": 1.0, "score": 139.0, "evalue": 5.57e-41, "gene_start": 1, "gene_end": 70, "reference_start": 76, "reference_end": 145, "reference_sequence": "MQLEYLNLISQAKVIAEKQFKANPFSFETIWKEVVKHFKISKQDEPSLIGRFYQDFLEDPNFVYLGDRKWKLRDFMKFDEWNKISQSMFVTKEIFEEGYEDLSNKKVEPEEGVGDFIMGNDGDDNETGSEIVQGLINDSFSEENQ" }, "paralog": false, "identity": 0.9862068965517241, "score": 287.0, "evalue": 6e-102, "description": "Internal stop codon at 26126, 26243. Nonsense mutation around 26126, 26243.", "psc": { "uniref90_id": "UniRef90_P47268", "gene": "rpoE", "product": "DNA-directed RNA polymerase subunit delta", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P47268", "cog_id": "COG3343", "cog_category": "K", "kegg_orthology_id": "K03048", "go_ids": [ "GO:0000428", "GO:0003899", "GO:0006351", "GO:0006355" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47268", "EC:2.7.7.6", "UniRef:UniRef50_P47268", "COG:COG3343", "COG:K", "KEGG:K03048", "GO:0000428", "GO:0003899", "GO:0006351", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_P47268", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47268" ], "product": "Probable DNA-directed RNA polymerase subunit delta" } }, "id": "JPCNHPFPBM_86", "locus": "GCA000292505_00046" }, { "type": "cds", "sequence": "contig_1", "start": 26500, "stop": 27030, "strand": "+", "frame": 1, "gene": null, "product": "Fructose-bisphosphate aldolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P7V3" ], "nt": "ATGCTTCAAAAAGCAAAGCTAGGTAAATATGCAATCCCTCACATTAACATCAATAACTATGAATGGGCCAAAGCTGTTTTAACAGCAGCAAATCAAGCTAATAGCCCAATTATTGTTTCAGTATCTGAAGGTGCTTTAAAGTACATGTCTGGTTATAGTGTTGTTATCCCGCTTGTTAAGGGTTTAATGGAATCACTAAGTGTTAAAGTACCAGTGACATTACATTTAGATCATGGTAGTTATGATGCATGTGTCCAAGCATTACAGGCTGGATTTAGTTCAGTAATGTTTGATGGTTCACATTTACCATTTGAAGAAAATTTCAATAAATCTAAAAAGTTAATAGAGATAGCACAAAAAACAAATGCTTCTGTTGAACTTGAAGTTGGTACTATTGGTGGAGAAGAAGATGGTGTTATAGGACAAGGTGAGTTAGCTAATGTTGATGAATGTAAACAAATCGCTAGTTTAAAACCAGATGCTTTAGCAGCAGGAATTGGTAATATCCATGGTATCTATCCTAAGAATTGA", "aa": "MLQKAKLGKYAIPHININNYEWAKAVLTAANQANSPIIVSVSEGALKYMSGYSVVIPLVKGLMESLSVKVPVTLHLDHGSYDACVQALQAGFSSVMFDGSHLPFEENFNKSKKLIEIAQKTNASVELEVGTIGGEEDGVIGQGELANVDECKQIASLKPDALAAGIGNIHGIYPKN", "aa_hexdigest": "96141a54be5bc76876f58f2675169bb7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FEF401E", "query_cov": 0.9034090909090909, "subject_cov": 0.9034090909090909, "identity": 0.5720000000000001, "score": 188.0, "evalue": 4.83e-58, "valid": false, "product": "class II fructose-bisphosphate aldolase", "ec_ids": [ "4.1.2.13" ], "uniref50_id": "UniRef50_A0A3B0P7V3", "kegg_orthology_id": "K01624" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P7V3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P7V3" ], "product": "Fructose-bisphosphate aldolase" }, "genes": [], "id": "JPCNHPFPBM_87", "locus": "GCA000292505_00047" }, { "type": "cds", "sequence": "contig_1", "start": 27049, "stop": 27345, "strand": "+", "frame": 1, "gene": null, "product": "fructose-bisphosphate aldolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A097ST37" ], "nt": "TTGATTGAAACAATATCAAAAATTACTAACTTGCCCTTAGTTTTACATGGTGGCTCTGGAATCTTAGAAAATGATGTTAAAAAAGCAATTAGTTTAGGGATTTGCAAACTAAATATTAATACTGAGTGTCAATTAGCATTTGCACATGAAATTAGAAAATACATTGAATCAAATAAAGACTTGGATCTTAACAAAAAAGGTTATGATCCTAGAAAACTTTTAAAAGAACCTACTCAAGCAATTGTTGATACTTGCTTGGAAAAGATTGATTTGTGTGGTTCTAGAAATAAAGCATAG", "aa": "MIETISKITNLPLVLHGGSGILENDVKKAISLGICKLNINTECQLAFAHEIRKYIESNKDLDLNKKGYDPRKLLKEPTQAIVDTCLEKIDLCGSRNKA", "aa_hexdigest": "9cdc981a63c4f3b529d80113279022e0", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A097ST37", "query_cov": 0.9795918367346939, "subject_cov": 0.9696969696969697, "identity": 0.635, "score": 124.0, "evalue": 3.83e-35, "valid": false, "product": "fructose-bisphosphate aldolase", "ec_ids": [ "4.1.2.13" ], "uniref50_id": "UniRef50_A0A097ST37", "kegg_orthology_id": "K01624" }, "pscc": { "uniref50_id": "UniRef50_A0A097ST37", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A097ST37" ], "product": "fructose-bisphosphate aldolase" }, "genes": [], "id": "JPCNHPFPBM_88", "locus": "GCA000292505_00048" }, { "type": "cds", "sequence": "contig_1", "start": 27346, "stop": 28233, "strand": "+", "frame": 1, "gene": "ychF", "product": "redox-regulated ATPase YchF", "db_xrefs": [ "COG:COG0012", "COG:J", "GO:0005524", "GO:0005525", "GO:0005737", "GO:0016887", "GO:0043023", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47270", "UniRef:UniRef90_P47270" ], "nt": "ATGTTAAGTGCAGGGATAGTTGGTTTACCTAATGTTGGTAAGTCAACTTTATTTAGTGCTATTACTAATTTGCAAGTTGAAATAGCAAACTATCCTTTTGCAACTATAGAACCTAATACTGGCATTGTTAATGTTAGTGATGAGAGATTAGATAAATTAGCTAGCTTAATTAATCCTGAAAAGATAGTTTATACAACCTTTCGTTTTGTTGATATAGCAGGTCTTGTTAAAGGCGCCAGTCAAGGTCAAGGATTGGGTAATCAATTCTTAGCAAACATCCGTGAAGTGGATTTAATTTGTCATGTTGTAAGATGTTTTCAAGATAAAAAAATTGTTCATGTTAACAATACAATAGATCCTGTTTTTGATTTTGAAATTATTGTTAATGAACTAATCCAAGCTGATTTTGAATTAATAACAAACAGAATCAGCAAACTTAAAAGAAAAGCTGAATCAGGTGATAAAATCGCTAAAGAAGAGTTTGTATTACTTGAAATTGTTTTAAATGGATTAAAACAAGGTCAAATGCCCATTCAGACTCTAAGTGAAAGTGAATTGAAAACAATTAAATCACTTAATCTATTAACAGCTAAACCTATTCTAATAGTAGCCAATGTATCTGAGAATGACTTATTAAACCTTGATAATAATGAAGCTTTAAAAAAATTGAATGCTTTTCTTGATCAAAAAAAGATTCCCAAGGCAATCACAGTTTGTTCTTTAATTGAAAAAGAATTAAGCGGTTTGAAATTAGAACAACGTCAATACTTTTTGGATGAACTTGGCTTAAAAAATTATTCAGGTTTAAACCAAGTAATTCAAGCTGCATATCAAACTTTAAACCTTTGGTCTTTTTTTACTTTTGGTAAAAAAGAAGTTAGAGCATGA", "aa": "MLSAGIVGLPNVGKSTLFSAITNLQVEIANYPFATIEPNTGIVNVSDERLDKLASLINPEKIVYTTFRFVDIAGLVKGASQGQGLGNQFLANIREVDLICHVVRCFQDKKIVHVNNTIDPVFDFEIIVNELIQADFELITNRISKLKRKAESGDKIAKEEFVLLEIVLNGLKQGQMPIQTLSESELKTIKSLNLLTAKPILIVANVSENDLLNLDNNEALKKLNAFLDQKKIPKAITVCSLIEKELSGLKLEQRQYFLDELGLKNYSGLNQVIQAAYQTLNLWSFFTFGKKEVRA", "aa_hexdigest": "90eaa26a2e7e2d88fa69f4313f57209c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47270", "query_cov": 1.0, "subject_cov": 0.8038147138964578, "identity": 0.993, "score": 562.0, "evalue": 6.96e-201, "valid": true, "gene": "ychF", "product": "redox-regulated ATPase YchF", "uniref50_id": "UniRef50_P47270", "cog_id": "COG0012", "cog_category": "J", "go_ids": [ "GO:0005524", "GO:0005525", "GO:0005737", "GO:0016887", "GO:0043023", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47270", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47270" ], "product": "Ribosome-binding ATPase YchF" }, "genes": [ "ychF" ], "id": "JPCNHPFPBM_89", "locus": "GCA000292505_00049" }, { "type": "cds", "sequence": "contig_1", "start": 28342, "stop": 28449, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF06071.19" ], "nt": "ATGAAATCTTTACAAGAAGCTAAAAAACAAGGTTTGATAAGATTGGAAGGCAAAAATTACTTAATAAAAGATGGTGATGTTTGTAACTTTAAATTTAACGTCACTTAA", "aa": "MKSLQEAKKQGLIRLEGKNYLIKDGDVCNFKFNVT", "aa_hexdigest": "6ae3cf5b3cd0c8373b031a994816ed93", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF06071.19", "name": "YchF-GTPase_C", "length": 32, "aa_cov": 0.9142857142857143, "hmm_cov": 1.0, "evalue": 3.4365137906278974e-11, "score": 38.27375411987305, "start": 2, "stop": 33 } ], "seq_stats": { "molecular_weight": 4029.6841000000013, "isoelectric_point": 9.360438728332518 }, "id": "JPCNHPFPBM_90", "locus": "GCA000292505_00050" }, { "type": "cds", "sequence": "contig_1", "start": 28652, "stop": 29305, "strand": "+", "frame": 2, "gene": "wcaA", "product": "putative glycosyltransferase MG025", "db_xrefs": [ "COG:COG0463", "COG:M", "EC:2.4.-.-", "GO:0016757", "SO:0001217", "UniRef:UniRef50_P75086", "UniRef:UniRef90_P47271" ], "nt": "GTGCCAAGTAAGTATTTATTCACCGTTATAATTCCAACATACAATTGTTGTCAATACATAAAAAAGGCATTAGATTCATTGCTTTTACAGAATGAATATTTTTTAAAAACACAAGTACTAATAGTTAACGATGGTTCACTAGACAATACTAAGGAAGTTGTTAGTGACTATTTAATAAAGTACTCCAATATTAGTTATTTTGAAAAAACTAATGGTAATTGGGGCAGTGTTATTAACTATGTTAAAAAAAATAAATTAGCCTTAGGTCAATATATTACTGTTTTAGATAGCGATGATTATTTTTTAAAAGATAGTTTTAAAAAAGTGGCTCGTTTCTTTGGCCATGACATGATCATTGGCGCTTTTTATTGTTATATTAATGAAAATAAAACTCGTTTTTTAAAACCTTATTTCGGTAAGACTGGTGTTATTAAAGAACATACCAAATTAAGAACCCCCCATTCCCAACCTATTGCTAAATTTTACAGTAATAAACTTTTCTATGAACTACATGATCTTAAGGAAAAGTTATTCTTCCAGGATTGTTTAATGTATCACGATGCTATTAACAGAGTTGAAAATGTTTTTTATTTGCGAGAGCCTTTAGCAGTTTGGTTTTCTACAAGACCTGGCAATTCTACAACAACTTCTTGA", "aa": "MPSKYLFTVIIPTYNCCQYIKKALDSLLLQNEYFLKTQVLIVNDGSLDNTKEVVSDYLIKYSNISYFEKTNGNWGSVINYVKKNKLALGQYITVLDSDDYFLKDSFKKVARFFGHDMIIGAFYCYINENKTRFLKPYFGKTGVIKEHTKLRTPHSQPIAKFYSNKLFYELHDLKEKLFFQDCLMYHDAINRVENVFYLREPLAVWFSTRPGNSTTTS", "aa_hexdigest": "fb1e3099d89ca6d3b8e3b24a376a841a", "start_type": "GTG", "rbs_motif": null, "genes": [ "wcaA" ], "truncated": "3-prime", "pseudogene": { "start": 28652, "stop": 29548, "observations": { "mutation": [ 29303, 29468 ], "stop": [ 29303, 29468 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47271", "query_cov": 1.0, "subject_cov": 0.7281879194630873, "identity": 0.995, "score": 444.0, "evalue": 9.98e-157, "gene_start": 1, "gene_end": 217, "reference_start": 1, "reference_end": 217, "reference_sequence": "MPSKYLFTVIIPTYNCCQYIKKALDSLLLQNEYFLKTQVLIVNDGSLDNTKEVVSDYLIKYSNISYFEKTNGNWGSVINYVKKNKLALGQYITVLDSDDYFLKDSFKKVARFFGHDMIIGAFYCYINENKTRFLKPYFGKTGVIKEHTKLRTPHSQPIAKFYSNKLFYELHDLKEKLFFQDCLMYHDAINRVESVFYLREPLAVWFSTRPGNSTTTSWENPNKFNAWCEILQKMNLYGAGIVIYIYTMLPGFLKQLKKKQLILNLNHKPAYTWLPKPLAFIFGGLMAFKTRKYIKYPK" }, "paralog": false, "identity": 0.9832214765100671, "score": 606.0, "evalue": 5.16e-223, "description": "Internal stop codon at 29303, 29468. Nonsense mutation around 29303, 29468.", "psc": { "uniref90_id": "UniRef90_P47271", "gene": "WcaA", "product": "Uncharacterized glycosyltransferase MG025", "ec_ids": [ "2.4.-.-" ], "uniref50_id": "UniRef50_P75086", "cog_id": "COG0463", "cog_category": "M", "go_ids": [ "GO:0016757" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47271", "EC:2.4.-.-", "UniRef:UniRef50_P75086", "COG:COG0463", "COG:M", "GO:0016757" ] }, "pscc": { "uniref50_id": "UniRef50_P75086", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75086" ], "product": "Uncharacterized glycosyltransferase MG025 homolog" } }, "id": "JPCNHPFPBM_91", "locus": "GCA000292505_00051" }, { "type": "cds", "sequence": "contig_1", "start": 29351, "stop": 29470, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTGTATGGAGCTGGGATAGTAATCTACATCTATACTATGCTACCTGGATTTCTAAAACAACTAAAGAAAAAAGAACTAATACTGAATTTGAACCATAAACCAGCTTACACTTGA", "aa": "MNLYGAGIVIYIYTMLPGFLKQLKKKELILNLNHKPAYT", "aa_hexdigest": "034fdddd1f30750c9811f7895d2205ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4511.438200000001, "isoelectric_point": 9.69941349029541 }, "id": "JPCNHPFPBM_92", "locus": "GCA000292505_00052" }, { "type": "cds", "sequence": "contig_1", "start": 29552, "stop": 30124, "strand": "+", "frame": 2, "gene": "efp", "product": "elongation factor P", "db_xrefs": [ "COG:COG0231", "COG:J", "GO:0003746", "GO:0005737", "GO:0006414", "GO:0043043", "KEGG:K02356", "RefSeq:WP_014894103.1", "SO:0001217", "UniParc:UPI00027B318F", "UniRef:UniRef100_UPI00027B318F", "UniRef:UniRef50_P75085", "UniRef:UniRef90_P47272" ], "nt": "ATGGCAGAAATGATAGAAGCAAAAAATCTTCGTAATGGGCAAACCATCTTCGGTCCTAACAAAGAGATTTTATTAGTACTGGAAAATACATTTAACAAAACCGCAATGCGCCAGGGAATTGTTAAAACTAAAGTTAAAAACTTAAGAACTGGGGCTATTGTTTGGCTTGAATTTACTGGTGACAAATTAGAACAAGTAATTATTGATAAGAAAAAAATGAATTTCTTATACAAAGATGGTAATAACTTTGTTTTTATGGATCAAAAAGACTACAGTCAGATTGAGATTAATGAAAAAAAATTAGAGTGGGAAAAAAATTTCATTACTGAAGAAATTGAAGTTACTGTTATTACTTATCAAGATGAAATTCTAGGAGTTAATTTACCTGATTTAGTTCCTATTGAAGTTGAGTTTGCTGAAGATGCTATTCAGGGCAATACTGCTAACATGGCAAGAAAAAAAGCACGCCTTGTAACTGGTTATGAACTTGATGTACCCCAATTTATTAATACTGGTGATAAGATTGTAATTGCCACTATTGATGGCAATTACCGTGAAAGGTTTAACAAATAA", "aa": "MAEMIEAKNLRNGQTIFGPNKEILLVLENTFNKTAMRQGIVKTKVKNLRTGAIVWLEFTGDKLEQVIIDKKKMNFLYKDGNNFVFMDQKDYSQIEINEKKLEWEKNFITEEIEVTVITYQDEILGVNLPDLVPIEVEFAEDAIQGNTANMARKKARLVTGYELDVPQFINTGDKIVIATIDGNYRERFNK", "aa_hexdigest": "4b720a55fbb962d599ccaf1d21c12b95", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B318F", "ncbi_nrp_id": "WP_014894103.1", "uniref100_id": "UniRef100_UPI00027B318F", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B318F", "RefSeq:WP_014894103.1", "UniRef:UniRef100_UPI00027B318F" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B318F", "uniref90_id": "UniRef90_P47272" }, "psc": { "uniref90_id": "UniRef90_P47272", "gene": "efp", "product": "elongation factor P", "uniref50_id": "UniRef50_P75085", "cog_id": "COG0231", "cog_category": "J", "kegg_orthology_id": "K02356", "go_ids": [ "GO:0003746", "GO:0005737", "GO:0006414", "GO:0043043" ] }, "pscc": { "uniref50_id": "UniRef50_P75085", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75085" ], "product": "Elongation factor P" }, "genes": [ "efp" ], "id": "JPCNHPFPBM_93", "locus": "GCA000292505_00053" }, { "type": "cds", "sequence": "contig_1", "start": 30093, "stop": 30260, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09185.15" ], "nt": "ATGGCAATTACCGTGAAAGGTTTAACAAATAAATTAACTAGAACCCAAAGACGAATTGCTGTAGTGGAATTTATCTTTTCTCTCCTCTTTTTTTTACCTAAAGAAGCTGAAGTTATTCAAGCAGATTTTTTAGAATATGATACTAAAGAACGACAACTAAATGAATGA", "aa": "MAITVKGLTNKLTRTQRRIAVVEFIFSLLFFLPKEAEVIQADFLEYDTKERQLNE", "aa_hexdigest": "fd22dea1bdfd44b29cbd46360442dd47", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09185.15", "name": "DUF1948", "length": 44, "aa_cov": 0.8, "hmm_cov": 1.0, "evalue": 8.811658025872916e-25, "score": 82.33567810058594, "start": 12, "stop": 55 } ], "seq_stats": { "molecular_weight": 6475.4695999999985, "isoelectric_point": 6.225256538391113 }, "id": "JPCNHPFPBM_94", "locus": "GCA000292505_00054" }, { "type": "cds", "sequence": "contig_1", "start": 30330, "stop": 30548, "strand": "+", "frame": 3, "gene": "nusB", "product": "putative protein MG027", "db_xrefs": [ "COG:COG0781", "COG:K", "SO:0001217", "UniRef:UniRef50_P47273", "UniRef:UniRef90_P47273" ], "nt": "TTGAAAAATCAATTAGAAATTCAAACTAAATACAATAAAATATCAGGAAAAAAGATTGATCTTTTAACTACTGCAGTTGTTTTATGTGCACTTAGTGAACAAAAGGCACATAATACTGATAAACCACTTTTAATTAGTGAGGCATTGTTGATTATGGATCGTTATTCACAAGGTGCTGAAAAAAAACAAACTCATGCTTTATTAGATAAGCTCTTGTAA", "aa": "MKNQLEIQTKYNKISGKKIDLLTTAVVLCALSEQKAHNTDKPLLISEALLIMDRYSQGAEKKQTHALLDKLL", "aa_hexdigest": "649c420bb67839b397dcf26686b71fca", "start_type": "TTG", "rbs_motif": null, "genes": [ "nusB" ], "truncated": "5-prime", "pseudogene": { "start": 30093, "stop": 30548, "observations": { "mutation": [ 30258 ], "stop": [ 30258 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47273", "query_cov": 1.0, "subject_cov": 0.4768211920529801, "identity": 0.972, "score": 137.0, "evalue": 2.95e-40, "gene_start": 1, "gene_end": 72, "reference_start": 80, "reference_end": 151, "reference_sequence": "MAITVKGLTNKLTRTQRRIAVVEFIFSLLFFLPKEAEVIQADFLEYDTKERQLNEWQKLIVKAFSENIFSFQKKIEEQQLKNQLEIQTKYNKISGKKIDLLTTAVVLCALSEQKAHNTDKPLLISEALLIMDHYSQGAEKKQTHALLDKLL" }, "paralog": false, "identity": 0.9867549668874173, "score": 283.0, "evalue": 2.63e-100, "description": "Internal stop codon at 30258. Nonsense mutation around 30258.", "psc": { "uniref90_id": "UniRef90_P47273", "gene": "NusB", "product": "Uncharacterized protein MG027", "uniref50_id": "UniRef50_P47273", "cog_id": "COG0781", "cog_category": "K", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47273", "UniRef:UniRef50_P47273", "COG:COG0781", "COG:K" ] }, "pscc": { "uniref50_id": "UniRef50_P47273", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47273" ], "product": "Uncharacterized protein MG027" } }, "id": "JPCNHPFPBM_95", "locus": "GCA000292505_00055" }, { "type": "cds", "sequence": "contig_1", "start": 30983, "stop": 31153, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAAAACTGCACATTTTTTTATTGATATAGAGATTTTGAAAGCCAAAAAAGGAGAACCCACTGATCCTAATAAAACTGATAATAGAGCAGTTGTTATTAATCTTGATGAGAATAAAAAGAATGAAAAAGAACAGTCCCCTCCTTCTGCAGAAATGACTAGTCTTTAA", "aa": "MSKTAHFFIDIEILKAKKGEPTDPNKTDNRAVVINLDENKKNEKEQSPPSAEMTSL", "aa_hexdigest": "54fd8293bb6a9a7188abedcdfb7798ba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6300.047800000002, "isoelectric_point": 5.7142740249633786 }, "id": "JPCNHPFPBM_96", "locus": "GCA000292505_00056" }, { "type": "cds", "sequence": "contig_1", "start": 31143, "stop": 31736, "strand": "-", "frame": 2, "gene": "yajL", "product": "putative protein MG029", "db_xrefs": [ "COG:COG0693", "COG:V", "GO:0005737", "SO:0001217", "UniRef:UniRef50_P47275", "UniRef:UniRef90_P47275" ], "nt": "ATGCTGGGGACCGTTTATTTGGTACTAAATAAAATGAAAAAGATACTTGTTATTGTCTATCCAGAGATGAATGATGTTGAATATACCAATGTAATGGTTGTATTTAGTTTCATTAAAACAATACAAACAACATGTTATCACCATTCATTAAAAAAGATAACTGCTAGTAATGGTGTTGTTGAAGTTAACAATATTGTCAACACCATTAACTTATCAGAATTTGATGCAGTTTATATTCCCGGTGGAATAGGAGCTACAAAACACTTAGATAAAGATGAAAAACTGCTTAAAACAATTAATTATTTCAAAGTAAATAACCTTTATCTTTTTGCTATTTGTGACACACCGAATGTTCTATTTAAACATGGCATTATCACTAAAGATGAGATCTATTCTTCTTTTCCAAACCCAAATTTAGTTATGTCTGAAAACAGATCAACAGCAAAAGTAACAGTAGCAAATAAATTAATAACAGCAAGAAGTGCTGGTTGTGCTTTGGAATTTGCAACAGTTATTGTGTGCACTTTTTTAAAAGATAACACTTTAAATGAACTTGTTCATAAAAGGTTATTTGGTAGTGAAATTAAAGACTAG", "aa": "MLGTVYLVLNKMKKILVIVYPEMNDVEYTNVMVVFSFIKTIQTTCYHHSLKKITASNGVVEVNNIVNTINLSEFDAVYIPGGIGATKHLDKDEKLLKTINYFKVNNLYLFAICDTPNVLFKHGIITKDEIYSSFPNPNLVMSENRSTAKVTVANKLITARSAGCALEFATVIVCTFLKDNTLNELVHKRLFGSEIKD", "aa_hexdigest": "6f4c7a7c877f5790514d8340318d40ec", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47275", "query_cov": 0.9441624365482234, "subject_cov": 1.0, "identity": 1.0, "score": 369.0, "evalue": 4.24e-129, "valid": true, "gene": "YajL", "product": "Uncharacterized protein MG029", "uniref50_id": "UniRef50_P47275", "cog_id": "COG0693", "cog_category": "V", "go_ids": [ "GO:0005737" ] }, "pscc": { "uniref50_id": "UniRef50_P47275", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47275" ], "product": "Uncharacterized protein MG029" }, "genes": [ "yajL" ], "id": "JPCNHPFPBM_97", "locus": "GCA000292505_00057" }, { "type": "cds", "sequence": "contig_1", "start": 31705, "stop": 32325, "strand": "-", "frame": 1, "gene": "upp", "product": "uracil phosphoribosyltransferase", "db_xrefs": [ "COG:COG0035", "COG:F", "EC:2.4.2.9", "GO:0000287", "GO:0004845", "GO:0005525", "GO:0005737", "GO:0006223", "GO:0044206", "RefSeq:WP_014894537.1", "SO:0001217", "UniParc:UPI00027B3191", "UniRef:UniRef100_UPI00027B3191", "UniRef:UniRef50_P47276", "UniRef:UniRef90_P47276" ], "nt": "GTGATAAAAAAAGTTCAACATGCTTTAATCTTGAATGAATTGACAAAACTGCGTGATAAGAATACAACAACCTCCCAGTTTCGCATGGCCTTGAATCAAATCACTTCATTACTCTTTTTTGAAGCAACTAAACAGCTACCACTAGCAATAGTTGAAGTTGAAACTCCCTTTGCCAAAACAAAGGGCTACAAATTAAAAAATGACATTGTTCTTGTACCTATTATGCGTGCTGGACTTGGAATGATTGATGCTATTGTTCGCTATTCAGATAAAATCAGAGTTGGTCATTTAGGAATCTATCGTCAAACCCAAACAACCAGTGTAATTTCATACTATAAAAAGATGCCTGAAAACATCTCTGATTCACATGTTATTATTCTTGATCCTATGCTTGCTACTGGAACTACATTGTTAACTGCTATTAAATCTATTAAAGAAGATAAACCTATCAAAATTAGTGTTATTGCTATAGTAGCAGCACCTGAAGGAATTAATAAAGTAGAAAAAATGCATCCTGATGTTGATATATTTCTTGCAGCAATTGATGAAAAGTTAAATGACAATAGATACATAATCCCTGGTCTTGGTGATGCTGGGGACCGTTTATTTGGTACTAAATAA", "aa": "MIKKVQHALILNELTKLRDKNTTTSQFRMALNQITSLLFFEATKQLPLAIVEVETPFAKTKGYKLKNDIVLVPIMRAGLGMIDAIVRYSDKIRVGHLGIYRQTQTTSVISYYKKMPENISDSHVIILDPMLATGTTLLTAIKSIKEDKPIKISVIAIVAAPEGINKVEKMHPDVDIFLAAIDEKLNDNRYIIPGLGDAGDRLFGTK", "aa_hexdigest": "6a871168bd19592a5c0d2917853cfcab", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3191", "ncbi_nrp_id": "WP_014894537.1", "uniref100_id": "UniRef100_UPI00027B3191", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3191", "RefSeq:WP_014894537.1", "UniRef:UniRef100_UPI00027B3191" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3191", "uniref90_id": "UniRef90_P47276" }, "psc": { "uniref90_id": "UniRef90_P47276", "gene": "upp", "product": "uracil phosphoribosyltransferase", "ec_ids": [ "2.4.2.9" ], "uniref50_id": "UniRef50_P47276", "cog_id": "COG0035", "cog_category": "F", "go_ids": [ "GO:0000287", "GO:0004845", "GO:0005525", "GO:0005737", "GO:0006223", "GO:0044206" ] }, "pscc": { "uniref50_id": "UniRef50_P47276", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47276" ], "product": "Uracil phosphoribosyltransferase" }, "genes": [ "upp" ], "id": "JPCNHPFPBM_98", "locus": "GCA000292505_00058" }, { "type": "cds", "sequence": "contig_1", "start": 32359, "stop": 32820, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12836.13", "PFAM:PF14579.12" ], "nt": "TTGAGTTACTATGCTTGTTTATTAAGTTTCAAACTAAAGGAACATGATATTAATGGTTTTGAAAAAGGGTATGAATTTATTAAAAACCGTTTAGATGAACTTAATAAGCTTTACAGAATAAAAAAGATTAAACCAAAAGAAGCGGAGCTTTTAACAAGTTATGAAGTTTATTTGGAAATGATGGCACGCAATATAAAGTTGCAACAAATCTCTATTCAAAACTCTAATGCAAGGATGTTTGTTGAACATAATGGTGTTTTAATTGCGCCTTTCATTACTATTCCAGGAATGGGAGAAGCAGTAGCTTCTTCAATTGTAGAAGCAAGAAATGAAAAACCATTTGCTTCCTTAAATGATTTCAAAAAAAGAACAAAAATTACTAAAAAACACGTTGAAACGATGGAACAATTACAACTGTTTGATGAATTTGAACACCAAGATGATCATAAACTTTTCAACTAA", "aa": "MSYYACLLSFKLKEHDINGFEKGYEFIKNRLDELNKLYRIKKIKPKEAELLTSYEVYLEMMARNIKLQQISIQNSNARMFVEHNGVLIAPFITIPGMGEAVASSIVEARNEKPFASLNDFKKRTKITKKHVETMEQLQLFDEFEHQDDHKLFN", "aa_hexdigest": "256d34ff6c898c33f9bb34d867885202", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12836.13", "name": "HHH_3", "length": 44, "aa_cov": 0.28104575163398693, "hmm_cov": 1.0, "evalue": 1.5106794693405254e-09, "score": 32.9807014465332, "start": 93, "stop": 135 }, { "id": "PF14579.12", "name": "HHH_6", "length": 85, "aa_cov": 0.5294117647058824, "hmm_cov": 0.9882352941176471, "evalue": 7.029067299647654e-17, "score": 56.59148406982422, "start": 65, "stop": 145 } ], "seq_stats": { "molecular_weight": 17992.598299999998, "isoelectric_point": 7.817001152038574 }, "id": "JPCNHPFPBM_99", "locus": "GCA000292505_00059" }, { "type": "cds", "sequence": "contig_1", "start": 32851, "stop": 33054, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTATCTAATTAATAAAGGGATGCAAGCAAAAGATGCTTTTGAAATCATGGAAAAAGTAAGAAAAGGTATTAAAGTAAATGCTAAAGAAGTTAGCCTGATGCAAAACTGTGGTGTTGAACAATATTGGATTAATGCTTGTTTAAAAATTAATTATCTATTCCCTAAGGCACATGCTGCAGCTTATGTTTTAATGGCTTGA", "aa": "MLYLINKGMQAKDAFEIMEKVRKGIKVNAKEVSLMQNCGVEQYWINACLKINYLFPKAHAAAYVLMA", "aa_hexdigest": "34910a29dfd04a42bf9e5e4df94ceae6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7654.1149000000005, "isoelectric_point": 9.230857276916506 }, "id": "JPCNHPFPBM_100", "locus": "GCA000292505_00060" }, { "type": "cds", "sequence": "contig_1", "start": 33130, "stop": 33420, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17657.6" ], "nt": "TTGAAACTTGACATCTTAGGTCAAGATGATCCAACAATGTTAAAGCACTTAGCTGATCTAACTAAGATAAATCCCCAAAATATCCCTCATTTTGATAAGAACTTAATTTCGATGTTTTCTTCAAACAAACCATTAAACCTAAAACCAGGCATTGTTGATGAAGTTACAGGAGCGGTTGGTATCCCTGAATTTGGGACTAAATTTGTTAGAAAAATTTTAGAACAGACAAAACCTAAAGACTTTGCTGACTTAATTAGAGTTTCAGGATTAAGCCATGGTAAAAACGTGTGA", "aa": "MKLDILGQDDPTMLKHLADLTKINPQNIPHFDKNLISMFSSNKPLNLKPGIVDEVTGAVGIPEFGTKFVRKILEQTKPKDFADLIRVSGLSHGKNV", "aa_hexdigest": "91a4552a00a42539e732c5ea8a3da7a4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17657.6", "name": "DNA_pol3_finger", "length": 92, "aa_cov": 0.8958333333333334, "hmm_cov": 0.8478260869565217, "evalue": 6.984108853550813e-19, "score": 62.86557388305664, "start": 10, "stop": 95 } ], "seq_stats": { "molecular_weight": 10636.28689999999, "isoelectric_point": 9.00508899688721 }, "id": "JPCNHPFPBM_101", "locus": "GCA000292505_00061" }, { "type": "cds", "sequence": "contig_1", "start": 33463, "stop": 33897, "strand": "-", "frame": 1, "gene": null, "product": "DNA polymerase III alpha subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380E0Y6" ], "nt": "ATGATTCGTGATTGTCCTAAATGCCATGAAAAAGCAAGTTTCAAAGGTGATGGTCATAACATTCCTTTTGCTACTTTTATGGGTTTTTCTGGTGATAAAATTCCTGATATCGATCTTAATTTTTCTAGCGAATATCAAGCTAAAGCCCATGATTATGTCAGAAAATTATTTGGAGTTAATAACACCTTCAGGGCAGGAACAATTGCAACAGTTGCTGAAAAAACTGCTTATGGTTATGCTAGAAACTATTTTGAAATCATTAAACGTGTTGATCTTGCAACTACTGCTGAAATTGAAAGATTTAAACAAAAGCTTATAGGAATTAAGAGAACAACAGGGCAACATCCAGGGGGAATTATGATCTTTCCTAGTGATCATTCTGTTTATGAATTTACTCCCTGTGGTTTTCCAGCAGATGATGTTGAAAGTGAATGA", "aa": "MIRDCPKCHEKASFKGDGHNIPFATFMGFSGDKIPDIDLNFSSEYQAKAHDYVRKLFGVNNTFRAGTIATVAEKTAYGYARNYFEIIKRVDLATTAEIERFKQKLIGIKRTTGQHPGGIMIFPSDHSVYEFTPCGFPADDVESE", "aa_hexdigest": "c63ff05b17895e59818e6c4bcc026882", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A380E0Y6", "query_cov": 0.8402777777777778, "subject_cov": 0.9453125, "identity": 0.562, "score": 140.0, "evalue": 3.12e-40, "valid": false, "product": "DNA polymerase III alpha subunit", "uniref50_id": "UniRef50_A0A380E0Y6" }, "pscc": { "uniref50_id": "UniRef50_A0A380E0Y6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380E0Y6" ], "product": "DNA polymerase III alpha subunit" }, "genes": [], "id": "JPCNHPFPBM_102", "locus": "GCA000292505_00062" }, { "type": "cds", "sequence": "contig_1", "start": 34195, "stop": 34365, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAAAAGAATGAGTTTTTTAGGAGAAGAGATTGCCTATAAATTAGTCGTTGAAAACACTAATAAGATAGTGAAGCTACTTGACTTAAATGAATTAGTACCAACTAAAAACAAGCTTTATCCACCAGTGATGCAAGATTCTAACCAAAAATTAATTGATAAAACTTGA", "aa": "MLKRMSFLGEEIAYKLVVENTNKIVKLLDLNELVPTKNKLYPPVMQDSNQKLIDKT", "aa_hexdigest": "96e71e0030c0200192c09eda84923f4d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6505.664500000003, "isoelectric_point": 9.049507713317869 }, "id": "JPCNHPFPBM_103", "locus": "GCA000292505_00063" }, { "type": "cds", "sequence": "contig_1", "start": 34435, "stop": 35109, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATCCCTTGTTTTCATCCCATACTTTGAGCAATATTTGTTCTAAACTAAAACTTGAATTTGATGATGAAAGATTACACCGTGCTGAATATGATACAGAAGCTTTAAAAAAAGTTTTCTTTTACTTTAAAAAGCAGTTAAAAGAAATGGGTATTAATACCTTAACAGAGATAGATCAAAACCTAAATAAAAAGTGTCAAATTGATCTGATGAAAAGGGTATTTACAAACACAGCAATCGTTTATGTAAAAAACCAAAGGGGCTTTCAAAACCTATATGAGATGTTATCAATTGCATTAACAGATCATAATGCTAACAGACCATTAGTTTTAGCTAGCAGCTTAGCTAAATTTAGAAAATCTTTTTTATTAACAGAAAACCCTGTTCAGGGTGACATCTTTAAAGCTGCATTAACAAAACCAATTAATGAACTTGAAAAAGCAATTGAAAAAGTTGACTTTGTGCTTATTTCACAACCCAATGCTTACCTTGGTTATACACTACGTGAAGGACTGAAAAAAGAACTTATTAATGATGCAATCAAACTAGTTATCAAAACAGCAACAAAACTTAAGAAATTAGTTGCTGTTGCATCTGATGCTTACTTTATCCATCCTTGGGAAAATGAGTATTATAAGGCAATAGTTTGTGCTAAGGGTTTAGGTGGGAAATGA", "aa": "MNPLFSSHTLSNICSKLKLEFDDERLHRAEYDTEALKKVFFYFKKQLKEMGINTLTEIDQNLNKKCQIDLMKRVFTNTAIVYVKNQRGFQNLYEMLSIALTDHNANRPLVLASSLAKFRKSFLLTENPVQGDIFKAALTKPINELEKAIEKVDFVLISQPNAYLGYTLREGLKKELINDAIKLVIKTATKLKKLVAVASDAYFIHPWENEYYKAIVCAKGLGGK", "aa_hexdigest": "b05e5e06f8842947487e95cec938c3d0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25642.648900000022, "isoelectric_point": 9.30048313140869 }, "id": "JPCNHPFPBM_104", "locus": "GCA000292505_00064" }, { "type": "cds", "sequence": "contig_1", "start": 35113, "stop": 35580, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13482.12", "PFAM:PF00929.31" ], "nt": "GTGGCTAAAAAATATGATTTAAAAGCTATCTATGGTTTGGAATTCAACTTAACTGATGATCACATCAAAATTGTTCACAATCCAGATAACACTAAGTTAAGTGATGCCACCTTTGTTATTTTTGATATTGAAACCACTGGATTACATGGTAGGTATGATGATGTTATTGAGTTTTCAGCACGCAAAATTAAGAATAACAGCGAGATAGATCATCAACAATTCTTTTTAAAAATTGACAAACCTATCCCAAAAACAATCACTGAAATCACCAAAATAACTGATGAGATGCTTGAAGGCGGTATTGATCAACAGCAAGGTTTAGAAAAGATAAGAAATTATCTAGATGGTTGTGTTATGGTATCTCATAATGGTATTAATTTTGATTTACCCTTTTTGCAAACTCAATTTGAAAAATACAACATCAAACCATTAACAAACCCGTTAATTGATACCTTATGTTTATCATGA", "aa": "MAKKYDLKAIYGLEFNLTDDHIKIVHNPDNTKLSDATFVIFDIETTGLHGRYDDVIEFSARKIKNNSEIDHQQFFLKIDKPIPKTITEITKITDEMLEGGIDQQQGLEKIRNYLDGCVMVSHNGINFDLPFLQTQFEKYNIKPLTNPLIDTLCLS", "aa_hexdigest": "35cbbb89820f8dd3fb19641f840bed97", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13482.12", "name": "RNase_H_2", "length": 112, "aa_cov": 0.7225806451612903, "hmm_cov": 0.8125, "evalue": 1.1467669957436216e-09, "score": 33.49589920043945, "start": 39, "stop": 150 }, { "id": "PF00929.31", "name": "RNase_T", "length": 124, "aa_cov": 0.7483870967741936, "hmm_cov": 0.9919354838709677, "evalue": 9.944135591774341e-25, "score": 83.07257843017578, "start": 39, "stop": 154 } ], "seq_stats": { "molecular_weight": 17835.154799999997, "isoelectric_point": 5.144974708557128 }, "id": "JPCNHPFPBM_105", "locus": "GCA000292505_00065" }, { "type": "cds", "sequence": "contig_1", "start": 35902, "stop": 36195, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATGCTGGTTTTAAAAACTTTAACTTCATTGCACTTTATCCTATTGATAAAAATGAATCTAAATTAAAAGTAAAAGCTGTAACTGTTTCTCAATATGATAAGCAGTTTGAAACAAAGGTATTTGCAACAGAATTTATCCCTATCCACAAGATTAACCAACAGATCGATGATGTCAAGATTATTGGGCAAATCTTTGAATTAAAAACCCATGAAAGTTTAACTGGTAAAAAAACACTTAATATCTATGTAACTGATTTTCAATTAGGTGGATCGTTAATTTTGAAATGA", "aa": "MNNAGFKNFNFIALYPIDKNESKLKVKAVTVSQYDKQFETKVFATEFIPIHKINQQIDDVKIIGQIFELKTHESLTGKKTLNIYVTDFQLGGSLILK", "aa_hexdigest": "429e5eb910b192178112f6fa5c312b6e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11109.755599999991, "isoelectric_point": 9.156976509094239 }, "id": "JPCNHPFPBM_106", "locus": "GCA000292505_00066" }, { "type": "cds", "sequence": "contig_1", "start": 36514, "stop": 36714, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTATTTAATCTTGAGAATGAAAAAGATAAAAAGGTTCTTGCACTCTCCAATTTTTTGATAGATAACAATATCCTTGACCACAATGAGCTTAACAGCTTAATTGAAAGGATTGAATCAATTTACTTTAATAAATATATTGAAGAGAAGGTTTTTTTATTTGCAATAACTATTAGTCGCCCTTTAACTATTGATATTTGA", "aa": "MVFNLENEKDKKVLALSNFLIDNNILDHNELNSLIERIESIYFNKYIEEKVFLFAITISRPLTIDI", "aa_hexdigest": "c41b364f725ab4630d87c3c3921fab6b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7815.942699999991, "isoelectric_point": 4.750114250183105 }, "id": "JPCNHPFPBM_107", "locus": "GCA000292505_00067" }, { "type": "cds", "sequence": "contig_1", "start": 36979, "stop": 37269, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACAATTCATAAACTATTTTTAGGTCTTTGTTTACCCGCAACATTAGGTCCTTTACTTGGAATTGTTGTTACAAATACTGACCAAAGTATTAAGTTTACAAGCAAATCGAATTCGATTAATAAGAATAACCAAAATAAAGAGTTGGCTCTACTTAGAGATAATTTGATGAACGAAGCGAAAGTTGATGAACCACTTTTCTTTGAAAAACGGTTTGAAAACTTTAAAAATAAGTATAGTGATATACATAGCTTAAATAACAGTGTTTTTTCACTTCATGACGTATGA", "aa": "MKTIHKLFLGLCLPATLGPLLGIVVTNTDQSIKFTSKSNSINKNNQNKELALLRDNLMNEAKVDEPLFFEKRFENFKNKYSDIHSLNNSVFSLHDV", "aa_hexdigest": "442da2f6d5b6ba1d8f1936841db963bf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10952.46939999999, "isoelectric_point": 8.78970012664795 }, "id": "JPCNHPFPBM_108", "locus": "GCA000292505_00068" }, { "type": "cds", "sequence": "contig_1", "start": 37259, "stop": 37957, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03086.20" ], "nt": "ATGACGTATGACTTATTAGGTGGATTTAAACAATCATTGACAACATTTTTTGATGAAGTGATTGCTCAACAACAAAAGATCAAGGATGCAGATAAGATCTTTCCAAGTACTAAAGATAATCCACCTAAAGAAGAAAATCCTAATGTTTTAGATACACTAGCTAACTACCAAGGAGCAGGATTTTTCCCTAGTTTAGGTAAAAATGGTTTTAATTTACCTGAAGCAGTGTTCCAAAATTTCACTGATTTTAGGATTAATGACTACAAGATTAAAAACTTTAATGTTGATCTTGTTAGTGAAAATGACATTATTAAACATGATAAAGTTCGTTATGCTTTTGAAGTTAAGTTCAATATTGCTTTAGTTTTATCTATTAATAAGTCAAATGTTGATTTTGACTTTGATTTCATTTTAAAGACTGATAATTTCTCAGACATTGAAAACTTTAATGAAATTTTCAACAGAAAACCTGCTTTACAATTTCGTTTTTATACCAAGATCAATGTACATAAGTTAAGTTTCAATGGTAGTGATTCCACTTACATTGCCAATATCTTGTTACAAGATCAGTTCAACCTATTAGAAATTGATTTAAATAAATCTATTTATGCATTAGATCTTGAAAATGCTAAAGAACGCTTTGATAATGAATTTGTTCAACCTCTTTATCAAAAACGACGTGAAGCTAAGCTTGCTTGA", "aa": "MTYDLLGGFKQSLTTFFDEVIAQQQKIKDADKIFPSTKDNPPKEENPNVLDTLANYQGAGFFPSLGKNGFNLPEAVFQNFTDFRINDYKIKNFNVDLVSENDIIKHDKVRYAFEVKFNIALVLSINKSNVDFDFDFILKTDNFSDIENFNEIFNRKPALQFRFYTKINVHKLSFNGSDSTYIANILLQDQFNLLEIDLNKSIYALDLENAKERFDNEFVQPLYQKRREAKLA", "aa_hexdigest": "23c2c2616e0d61dfeab2bfbccaf92386", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03086.20", "name": "DUF240", "length": 202, "aa_cov": 0.8663793103448276, "hmm_cov": 1.0, "evalue": 1.755343448883048e-93, "score": 307.38348388671875, "start": 3, "stop": 203 } ], "seq_stats": { "molecular_weight": 27022.108500000024, "isoelectric_point": 5.086089515686035 }, "id": "JPCNHPFPBM_109", "locus": "GCA000292505_00069" }, { "type": "cds", "sequence": "contig_1", "start": 38312, "stop": 38440, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGAGATGATAAAGCATTTCAATATGCTTTTGGTTTAGGTCCAAATACTTCTGAACAACAACTTGGTAGAGTAACCCTACCTGGTTATGGTTATGAAGGAATTAGAATGAGTGATTGGTTGAGATGA", "aa": "MRDDKAFQYAFGLGPNTSEQQLGRVTLPGYGYEGIRMSDWLR", "aa_hexdigest": "e72461b6d06e8627bac519a5b66a847d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4825.3553, "isoelectric_point": 6.0008562088012685 }, "id": "JPCNHPFPBM_110", "locus": "GCA000292505_00070" }, { "type": "cds", "sequence": "contig_1", "start": 38737, "stop": 38979, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTTACTATAGATTACATACAACTCCACCACAATATCGTTTAACTGATGATATGAAATTATTGTTTGTTCCGCACAGTATCCAAAGGGTAACTGCAGTTGGTAATGAAAGCATTAATGGTCTTCTCAGATCACAAAACCTTCATAACTTGGAACGTCAATCATATGAAGCGACTGCTCCTATTGATTTAATATCATATATGCTTTATGCAATTAGTGATAAAAAACCACCGCAAAAATAA", "aa": "MPYYRLHTTPPQYRLTDDMKLLFVPHSIQRVTAVGNESINGLLRSQNLHNLERQSYEATAPIDLISYMLYAISDKKPPQK", "aa_hexdigest": "553ab238800d5e6fbb73df9d9f67e94f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9288.560799999996, "isoelectric_point": 9.048605155944824 }, "id": "JPCNHPFPBM_111", "locus": "GCA000292505_00071" }, { "type": "cds", "sequence": "contig_1", "start": 39536, "stop": 39688, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATTGTACAAGTCCTAGTGTATTTAACATTCCTAGGAATTTTCTAACTGAATTTATTGCAACTTTAATATTGATAGGTGGAGTTGTTGCTGCTAGTCACTTTCTTCATAACAACCCAAACTCTGTTCCTCCTGGATTTATGGCGCTTTGA", "aa": "MHCTSPSVFNIPRNFLTEFIATLILIGGVVAASHFLHNNPNSVPPGFMAL", "aa_hexdigest": "1014036dc127e28fb885283ff7d6c691", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5394.254599999999, "isoelectric_point": 6.804616355895996 }, "id": "JPCNHPFPBM_112", "locus": "GCA000292505_00072" }, { "type": "cds", "sequence": "contig_1", "start": 40540, "stop": 41067, "strand": "+", "frame": 1, "gene": "hisS", "product": "histidine--tRNA ligase", "db_xrefs": [ "COG:COG0124", "COG:J", "EC:6.1.1.21", "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427", "SO:0001217", "UniRef:UniRef50_P47281", "UniRef:UniRef90_P47281" ], "nt": "ATGAACTTCTTGCAAAAACCAAGGGGAGTTAAAGATTGGTTTGGTGATGAATTAGTTTATTTTAATTGGATTGTTAAAAAAATAAGATCTTTAGCATTTAATTGGGGTTTTAGTGAAGTTAAAACTCCGTTGTTTGAAAATGCACAACTTTTTCAAAGATCTAATGCTAATGCTGATATTGTTCAAAAAGAACTATACCAGTTTTTTGATAAATCTCAAAGAGAATTAGCTTTAAGACCTGAAGCTACTACACCAATAGTAAGACTTGCTTGTGAAAACAAATTAATGCAAGAAGCAAATTTTCCCTTAAAGTTATTTTGCATTGGTTCAATGTATCGTTATGAACGTCCACAAAACAATAGGTTTCGTGAACATTGGCAATTTAGTTGCGAAGTATTTGGTTTTTCCAACCTGTTTATCTTTTTAGATACACTTTTGTTTGCTAACTCTTTGCTTGAAGCACTTGGAATTACTGGATATGTGCTTAAAATTAATAATCTTGCTAACTTTGAAACACTTAGTAAGTGA", "aa": "MNFLQKPRGVKDWFGDELVYFNWIVKKIRSLAFNWGFSEVKTPLFENAQLFQRSNANADIVQKELYQFFDKSQRELALRPEATTPIVRLACENKLMQEANFPLKLFCIGSMYRYERPQNNRFREHWQFSCEVFGFSNLFIFLDTLLFANSLLEALGITGYVLKINNLANFETLSK", "aa_hexdigest": "d5e7ed0c8f541ae38241a30e55e7c24a", "start_type": "ATG", "rbs_motif": null, "genes": [ "hisS" ], "truncated": "3-prime", "pseudogene": { "start": 40540, "stop": 41370, "observations": { "mutation": [ 41065, 41293 ], "stop": [ 41065, 41293 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47281", "query_cov": 1.0, "subject_cov": 0.4227053140096618, "identity": 1.0, "score": 357.0, "evalue": 3.24e-121, "gene_start": 1, "gene_end": 175, "reference_start": 1, "reference_end": 175, "reference_sequence": "MNFLQKPRGVKDWFGDELVYFNWIVKKIRSLAFNWGFSEVKTPLFENAQLFQRSNANADIVQKELYQFFDKSQRELALRPEATTPIVRLACENKLMQEANFPLKLFCIGSMYRYERPQNNRFREHWQFSCEVFGFSNLFIFLDTLLFANSLLEALGITGYVLKINNLANFETLSKWNKALKDYLTPYKLELTELSQKRLEKNPLRILDDKIDQKKSFVKNAPKITDFLDASAKQDSELLKTQLKKHNISFEWTDNLVRGLDYYTGFVFEYVKNQDTILAGGVYDNLVEELSSNPTPALGFACGIERLINCLEIDKKAFILNTKPKQMLVICLFEEALEELVWLAKLWREYNQVTIYPKVIKVDNGIRLANRLGYTFIGIVGKTDFDKKAITIKNLVSKQQTIYTWNELGERNVF" }, "paralog": false, "identity": 0.9891304347826086, "score": 550.0, "evalue": 8.35e-200, "description": "Internal stop codon at 41065, 41293. Nonsense mutation around 41065, 41293.", "psc": { "uniref90_id": "UniRef90_P47281", "gene": "hisS", "product": "histidine--tRNA ligase", "ec_ids": [ "6.1.1.21" ], "uniref50_id": "UniRef50_P47281", "cog_id": "COG0124", "cog_category": "J", "go_ids": [ "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47281", "EC:6.1.1.21", "UniRef:UniRef50_P47281", "COG:COG0124", "COG:J", "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427" ] }, "pscc": { "uniref50_id": "UniRef50_P47281", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47281" ], "product": "Histidine--tRNA ligase" } }, "id": "JPCNHPFPBM_113", "locus": "GCA000292505_00073" }, { "type": "cds", "sequence": "contig_1", "start": 41149, "stop": 41295, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGAATTTTAGATGACAAGATAGATCAAAAAAAATCATTTTTTAAAAATGCTCCTAAAATTACTGATTTTTTAGATGCAAGTGCAAAACAAGATTCAGAATTGTTAAAAACACAACTAAAAAAACACAATATTAGTTTTGAATGA", "aa": "MRILDDKIDQKKSFFKNAPKITDFLDASAKQDSELLKTQLKKHNISFE", "aa_hexdigest": "5bab1062afbd8b669913054749b4e2a7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5625.411699999999, "isoelectric_point": 9.10056667327881 }, "id": "JPCNHPFPBM_114", "locus": "GCA000292505_00074" }, { "type": "cds", "sequence": "contig_1", "start": 41317, "stop": 41565, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13393.11" ], "nt": "TTGGATTACTATACTGGATTTGTGTTTGAATATGTAAAAAATCAAGACACAATTCTAGCAGGTGGAGTTTATGATAACTTAGTTGAAGAATTAAGTAGTAATCCAACTCCCGCATTAGGTTTTGCTTGTGGGATTGAACGGTTAATTAACTGTTTAGAAACTGATAAAAAAGCATTCATTTTGAATACTAAACCAAAGCAGATGTTAGTAATTTGCTTATTTGAAGAAGCACTTGAAGAATTGATTTGA", "aa": "MDYYTGFVFEYVKNQDTILAGGVYDNLVEELSSNPTPALGFACGIERLINCLETDKKAFILNTKPKQMLVICLFEEALEELI", "aa_hexdigest": "91a006e5d93a80b2b2a0d1e4b4a734d5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13393.11", "name": "tRNA-synt_His", "length": 49, "aa_cov": 0.573170731707317, "hmm_cov": 0.9795918367346939, "evalue": 2.694131349380188e-10, "score": 35.041297912597656, "start": 2, "stop": 48 } ], "seq_stats": { "molecular_weight": 9255.591899999998, "isoelectric_point": 4.2809652328491214 }, "id": "JPCNHPFPBM_115", "locus": "GCA000292505_00075" }, { "type": "cds", "sequence": "contig_1", "start": 42350, "stop": 42886, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGATTGCAGGAGTTGAAAAGTATTTTCAAATTGCTAGGGTCTATCGTGATGAAGATAGCAGAAAAGATCGTCAACCAGAACACACACAAATTGATTTTGAGATCTCTTTTTGTAACCAAAAAATGATTATGAATCTAGTTGAAAAACTCTTTTTTAGTGTTTTCTTAGATGTTTTTCAAATCAAAATAAAAAAGACTTTTCCTGTTTTTAAATTTTCAGAACTTTTTGAAAGATTTGGTAGCGATAAACCAGATTTACGTTATGGTTTTGAAATAAAAGATTTCACCTCACTTTTTCAAGATCATCAAAATCAGTTCACTAAATTAATTGAAGCAAAAGGCATTATTGGTGGTATTGAACTTATTAATATTGAGTTAAGTACAGACAAAATTAAAGCATTAAGAAAAATTGCTAAGGACCATGATGTGAGTTTAGAAGTTCATAATAAAAATAATTCAACATTAAAAACTTCAATTAAATGTGATGAAAAAAACACCCTTCTGTTAGTAGCAAATAAATCTAAAAAGAAGGCATGA", "aa": "MIAGVEKYFQIARVYRDEDSRKDRQPEHTQIDFEISFCNQKMIMNLVEKLFFSVFLDVFQIKIKKTFPVFKFSELFERFGSDKPDLRYGFEIKDFTSLFQDHQNQFTKLIEAKGIIGGIELINIELSTDKIKALRKIAKDHDVSLEVHNKNNSTLKTSIKCDEKNTLLLVANKSKKKA", "aa_hexdigest": "681b245c90515608e83902314b0c80bf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 115, "aa_cov": 0.6179775280898876, "hmm_cov": 0.8608695652173913, "evalue": 2.7303357810396228e-30, "score": 100.5353775024414, "start": 1, "stop": 110 } ], "seq_stats": { "molecular_weight": 20817.859400000023, "isoelectric_point": 9.081935310363772 }, "id": "JPCNHPFPBM_116", "locus": "GCA000292505_00076" }, { "type": "cds", "sequence": "contig_1", "start": 43178, "stop": 43402, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGAATTCTTTGAACATTGACCCAAATAAGTTTGCTTTTCTTCTAGAAGCTTATGAATATGGTGCTCCTGTTCATGGTGGAATGGGACTAGGTATTGATCGTTTAATGATGATTCTTAATCAAACTGATAACATCAGAGAAGTAATCGCTTTTCCTAAGAATAATCATGGTATTGAAGTCCATACAAATGCTCCTGATAAAATTGAAAAAGAGGAGGTTAAATGA", "aa": "MNSLNIDPNKFAFLLEAYEYGAPVHGGMGLGIDRLMMILNQTDNIREVIAFPKNNHGIEVHTNAPDKIEKEEVK", "aa_hexdigest": "9936f54089a44145268b1cf5d28d6269", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 52, "aa_cov": 0.6486486486486487, "hmm_cov": 1.0, "evalue": 5.587874891509329e-20, "score": 66.67044830322266, "start": 7, "stop": 54 } ], "seq_stats": { "molecular_weight": 8323.431499999999, "isoelectric_point": 5.17242794036865 }, "id": "JPCNHPFPBM_117", "locus": "GCA000292505_00077" }, { "type": "cds", "sequence": "contig_1", "start": 43402, "stop": 43563, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF18127.6" ], "nt": "ATGGATAAAAGAACTAGTGAAATAGAGTTTCACTTAAAGAATCTTTTGGCTTGTGATGCATATAAACTTTCACACCGTTTAATGTATCCACAAGATACACAAAACCTTTATAGTATGTTAACTGCAAGAGGTGTTTATGGTGATTTTAAGGAGTTTGTTTGA", "aa": "MDKRTSEIEFHLKNLLACDAYKLSHRLMYPQDTQNLYSMLTARGVYGDFKEFV", "aa_hexdigest": "1a29b07947f892e4796a2ab69a699fd3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF18127.6", "name": "NAMPT_N", "length": 37, "aa_cov": 0.6981132075471698, "hmm_cov": 0.972972972972973, "evalue": 5.274129842025671e-07, "score": 25.006837844848633, "start": 15, "stop": 51 } ], "seq_stats": { "molecular_weight": 6289.158400000001, "isoelectric_point": 6.702988243103028 }, "id": "JPCNHPFPBM_118", "locus": "GCA000292505_00078" }, { "type": "cds", "sequence": "contig_1", "start": 43644, "stop": 44255, "strand": "+", "frame": 3, "gene": "pncB", "product": "nicotinate phosphoribosyltransferase", "db_xrefs": [ "COG:COG1488", "COG:H", "EC:2.4.2.12", "EC:6.3.4.21", "GO:0004514", "GO:0009435", "GO:0047280", "SO:0001217", "UniRef:UniRef50_P75067", "UniRef:UniRef90_P47283" ], "nt": "TTGCTAGCTGCAGCATTGACAGATAAATTAGTTAGTGTTTTTAATGATCATGAATTGGCTAATGAATTCACACAACACATCTGTCATTTAGCTAGTTTCTTAGAGAAAAATAAAAAAATGCCGTTAGTTGCAAAGATCCATGAAAGTGATCAATCATTACCATTTAGAACTCCTTTAATAACTATAGAAGGAGTTGAAAATATTCCAAACAACTTTGTATGGTTAGTTAATTACTTTGAAACTGTACTTCTAGAAAACATTTGGTTGTTTCAAACTGCTTCTACAGTTGCTAAAAGAATTAAATCTTTACTTGAAAAATATGCTAAAGAAACCGCAGATGAAACAAGTTTTATTAATTTTCAATGCCACGACTTTAGTATGCGGGGCATGAGTAGTTTGCAAAGTGCTTTGTATGTTGCTAGAGCACACTTGCAATACTTTACTGGAAGTGACACGATCTTAGGTGGGGATAATTCTCGTTCAATTTTAGCTTCTGAACATTCAGTGATGTGCGCAGATGGTAGTAAACATGAATTGAAAACTTTTCAACGTTTATTGGAAAAGTTTAAAGATAAAAAACTTTCTTTAGTGATTGATTCTTATGACATGTGA", "aa": "MLAAALTDKLVSVFNDHELANEFTQHICHLASFLEKNKKMPLVAKIHESDQSLPFRTPLITIEGVENIPNNFVWLVNYFETVLLENIWLFQTASTVAKRIKSLLEKYAKETADETSFINFQCHDFSMRGMSSLQSALYVARAHLQYFTGSDTILGGDNSRSILASEHSVMCADGSKHELKTFQRLLEKFKDKKLSLVIDSYDM", "aa_hexdigest": "858210f676b63d46ea12cef305bce634", "start_type": "TTG", "rbs_motif": null, "genes": [ "pncB" ], "truncated": "both", "pseudogene": { "start": 43399, "stop": 44558, "observations": { "deletion": [ 43636 ], "mutation": [ 43561 ], "stop": [ 43561, 44254, 44395 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47283", "query_cov": 1.0, "subject_cov": 0.45111111111111113, "identity": 0.995, "score": 402.0, "evalue": 4.06e-138, "gene_start": 1, "gene_end": 203, "reference_start": 83, "reference_end": 285, "reference_sequence": "MMDKRTSEIEFHLKNLLACDAYKLSHRLMYPQDTQNLYSMLTARGVYGDFKEFVWNHDFAKEILLNVFNGFVNSVIEVKKNKLLAAALTDKLVSVFNDHELANEFTQHICHLASFLEKNKKMPLVAKIHESDQSLPFRTPLITIEGVENIPNNFVWLVNYFETVLLENIWLFQTASTVAKRIKSLLEKYAKETADETSFINFQCHDFSMRGMSSLQSALYVARAHLQYFTGSDTILGGDNSRSILASEHSVMCADGSKHELKTFQRLLEKFKDKKLSLVIDSYDMWNVLDNIIPRLKNLILMRGATLYLRADSGNYQTLICNPNYKKQDKSTWAMIDYLDHHFSSTINKKGYKVLNKKIGIIYGDGITYQKIEWILNCLKNHGYCSSNIIFGVGSSTYQNLNRDTLGFVYKLTAIKRNNRWIGVKKTPITDLSKSSKGGRYKTKRLITVY" }, "paralog": false, "identity": 0.9922279792746114, "score": 762.0, "evalue": 1.24e-282, "description": "Internal stop codon at 43561, 44254, 44395. Frameshift due to deletion around 43636. Nonsense mutation around 43561.", "psc": { "uniref90_id": "UniRef90_P47283", "gene": "PncB", "product": "nicotinate phosphoribosyltransferase", "ec_ids": [ "2.4.2.12", "6.3.4.21" ], "uniref50_id": "UniRef50_P75067", "cog_id": "COG1488", "cog_category": "H", "go_ids": [ "GO:0004514", "GO:0009435", "GO:0047280" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47283", "EC:2.4.2.12", "EC:6.3.4.21", "UniRef:UniRef50_P75067", "COG:COG1488", "COG:H", "GO:0004514", "GO:0009435", "GO:0047280" ] }, "pscc": { "uniref50_id": "UniRef50_P75067", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75067" ], "product": "Nicotinamide phosphoribosyltransferase" } }, "id": "JPCNHPFPBM_119", "locus": "GCA000292505_00079" }, { "type": "cds", "sequence": "contig_1", "start": 44301, "stop": 44396, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTGGTGCTACGCTTTATTTGCGTGCTGATTCTGGTAATTATCAAACTCTTATTTGCAATCCTAATTACAAAAAGCAAGATAAAAGTACATGA", "aa": "MRGATLYLRADSGNYQTLICNPNYKKQDKST", "aa_hexdigest": "38cc5b37f7e62f36e580fa085a08b84f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3550.973400000001, "isoelectric_point": 9.428839683532715 }, "id": "JPCNHPFPBM_120", "locus": "GCA000292505_00080" }, { "type": "cds", "sequence": "contig_1", "start": 44400, "stop": 44750, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCGATTACTTAGATCATCATTTTAGTTCAACTATAAATAAAAAAGGTTATAAGGTTTTAAACAAGAAAATTGGCATTATTTATGGTGATGGAATCACCTATCAAAAGATAGAATGGATCTTAAATTGTTTAAAAAACCATGGTTATTGTTCTTCAAACATTATTTTTGGAGTTGGTAGTAGCACTTATCAAAATTTAAACCGTGATACTTTAGGTTTTGTATACAAATTAACTGCTATTAAAAGAAATAATAGATGGATAGACGTTAAAAAAACTCCCATAACTGATCTATCTAAAAGTTCAAAAGGCGGTAGATATAAAACAAAGCGATTAATTACAGTTTATTAA", "aa": "MIDYLDHHFSSTINKKGYKVLNKKIGIIYGDGITYQKIEWILNCLKNHGYCSSNIIFGVGSSTYQNLNRDTLGFVYKLTAIKRNNRWIDVKKTPITDLSKSSKGGRYKTKRLITVY", "aa_hexdigest": "68b50b170f6a93428f40ed9c0fa6f3de", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13386.364899999977, "isoelectric_point": 9.868965339660644 }, "id": "JPCNHPFPBM_121", "locus": "GCA000292505_00081" }, { "type": "cds", "sequence": "contig_1", "start": 44888, "stop": 45067, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02782.22" ], "nt": "ATGGCAAGTGATATAGGTTATAAGATTACTAGCATTAAAGCTGATGGGGGGATTGTTAAATCAAACTATTTAATGCAGTTTCAAGCTGATATTGCAGATGTAATTGTTTCTATCCCTAAAAATAAAGAAACCACTGCAGTTGGTGTTTGTTTTTTAGCTGGACTTGCTTGTGGATTTTGA", "aa": "MASDIGYKITSIKADGGIVKSNYLMQFQADIADVIVSIPKNKETTAVGVCFLAGLACGF", "aa_hexdigest": "123f07ce7d0e33d510412f30b14352eb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02782.22", "name": "FGGY_C", "length": 53, "aa_cov": 0.8983050847457628, "hmm_cov": 1.0, "evalue": 1.1979519526247282e-12, "score": 43.11842346191406, "start": 4, "stop": 56 } ], "seq_stats": { "molecular_weight": 6185.1698, "isoelectric_point": 5.950326347351075 }, "id": "JPCNHPFPBM_122", "locus": "GCA000292505_00082" }, { "type": "cds", "sequence": "contig_1", "start": 45185, "stop": 45337, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGTAGCGGGTGCGGCTATAAAATGGTTAAGGGATGCATTAAAAATTATCTATTCAGAAAAGGAAAGTGATTTTTATGCAGAACTTGCAAAAGAAAATGAACAAAACCTAGTTTTTGTACCAGCTTTCAGTGGACTTGGAGCTCCTTGA", "aa": "MFVAGAAIKWLRDALKIIYSEKESDFYAELAKENEQNLVFVPAFSGLGAP", "aa_hexdigest": "8fa1bd9ff07e691a8f53295fd4c1430b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5549.310100000001, "isoelectric_point": 4.7668249130249025 }, "id": "JPCNHPFPBM_123", "locus": "GCA000292505_00083" }, { "type": "cds", "sequence": "contig_1", "start": 45603, "stop": 45863, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAACTCAGAACTTTAGGTAAGATTGAAACTGGTACTTCAAATAAATCACATAACTCTTTGGATCACTCCATTTTGACAATGTCAAATAAAAGAGTTCTTGAAGCATTTGAAACATCTGTAACATGCATTTTTCCATTAGTTAGTTTTCAGATTAATCAGCTATCAATGGTGCCAAATAACAACTTTTTTTGCTCCATTAGTTTCTTTGCTAAAGGAACATTTTTTAAGATTCAAGCTATCTTAGTAGCACTAAAATAG", "aa": "MELRTLGKIETGTSNKSHNSLDHSILTMSNKRVLEAFETSVTCIFPLVSFQINQLSMVPNNNFFCSISFFAKGTFFKIQAILVALK", "aa_hexdigest": "36179cddd9d56581e09050ccd2654431", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9647.159099999992, "isoelectric_point": 9.18437557220459 }, "id": "JPCNHPFPBM_124", "locus": "GCA000292505_00084" }, { "type": "cds", "sequence": "contig_1", "start": 46001, "stop": 46090, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAGTGCTAAAAATAAAGCACAAATCAAATCTCATGAAGTGATTGCAGTTGGTATTACCAATCAAAGAGAAACAATAGTTTTATGA", "aa": "MQSAKNKAQIKSHEVIAVGITNQRETIVL", "aa_hexdigest": "159464c92f69e0a07a6228989c9a85cf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3207.7024, "isoelectric_point": 9.697157096862792 }, "id": "JPCNHPFPBM_125", "locus": "GCA000292505_00085" }, { "type": "cds", "sequence": "contig_1", "start": 46136, "stop": 46273, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCTAAAAAAACAATACATCATTGCCTTAGATGAAGGTACTAGTTCTTGTCGATCAATTGTTTTTGATCACAACCTTAACCAAATAGCAATAGCACAAAACGAATTTAACACTTTTTTTCCTAATAGTGGTTGA", "aa": "MDLKKQYIIALDEGTSSCRSIVFDHNLNQIAIAQNEFNTFFPNSG", "aa_hexdigest": "355f63ae6572bcf6c847359ea0119fdc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5091.643299999999, "isoelectric_point": 4.8263353347778315 }, "id": "JPCNHPFPBM_126", "locus": "GCA000292505_00086" }, { "type": "cds", "sequence": "contig_1", "start": 46294, "stop": 46668, "strand": "-", "frame": 1, "gene": null, "product": "Glycerol-3-phosphate dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B2Q2" ], "nt": "ATGGTACCTACTATCCATGGTAAGGGAGTTGTTGTTGCTGAGATGTTAGATGGTAATATTCTTGTGGGACCTAATGCAGTTGAGGGAATTGAAAAAAACAAAACTCGTTCCATAGATTTAGATTCAATTAACCAAATTAAAACTATTGGTAAAAAAATGGTTCCTTCATTGCAATTTGAAAATAGTATTTATAGTTTTGCAGGTTCAAGAGCTATTGATATTGAAACAAATGACTTTGTTATTAGAACAGCAAAAAGCAATCCTAACTTTATCATCCTTGGTGGCATGAAATCCCCTGGCTTAACATCAAGTCCTGCTATTGCTAAGAGAGCAGTTGAACTCCTTAATTTAAAGTTAAAAAAGAAGATAAATTGA", "aa": "MVPTIHGKGVVVAEMLDGNILVGPNAVEGIEKNKTRSIDLDSINQIKTIGKKMVPSLQFENSIYSFAGSRAIDIETNDFVIRTAKSNPNFIILGGMKSPGLTSSPAIAKRAVELLNLKLKKKIN", "aa_hexdigest": "e7198bb38adc51876e45e2edfef62437", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FF40EBC", "query_cov": 0.9919354838709677, "subject_cov": 0.8601398601398601, "identity": 0.561, "score": 130.0, "evalue": 1.02e-36, "valid": false, "product": "FAD-dependent oxidoreductase", "uniref50_id": "UniRef50_A0A449B2Q2" }, "pscc": { "uniref50_id": "UniRef50_A0A449B2Q2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B2Q2" ], "product": "Glycerol-3-phosphate dehydrogenase" }, "genes": [], "id": "JPCNHPFPBM_127", "locus": "GCA000292505_00087" }, { "type": "cds", "sequence": "contig_1", "start": 46972, "stop": 47106, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAAAGAAAGGGGAATTAAAAACTCAATTCCTGTTGAAAACATTCAAATATTAGACCAACAACAAACTTTATTGCAAGAACCGTTTATCAATCCAAATGTAGTAGCTAGTTTAAAAGTTGAAGGTAGTTGA", "aa": "MLKERGIKNSIPVENIQILDQQQTLLQEPFINPNVVASLKVEGS", "aa_hexdigest": "e895ac8fa1d30d23f4d04a1af7da3792", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4932.6494, "isoelectric_point": 5.002479362487793 }, "id": "JPCNHPFPBM_128", "locus": "GCA000292505_00088" }, { "type": "cds", "sequence": "contig_1", "start": 47200, "stop": 47418, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01266.30", "PFAM:PF13450.12", "PFAM:PF00070.33" ], "nt": "ATGCAAACAATTGACGTTTTAATTGTGGGTGGGGGTGTCATCGGTACTAGTTGTGCATATGAACTCAGCCAATATAAATTAAAAGTTGCACTTTTAGAAAAAAATGCTTTTTTGGGTTGTGAAACTTCGCAGGCTAACAGTGGTGTAATCCATTCAGGAATTGATCCTAATCCTAATAAGCTAACTGCCAAATACAACATCTTAGGGAGAAAAATCTGA", "aa": "MQTIDVLIVGGGVIGTSCAYELSQYKLKVALLEKNAFLGCETSQANSGVIHSGIDPNPNKLTAKYNILGRKI", "aa_hexdigest": "c6e04e1b2c34c60998973590ae6100c7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01266.30", "name": "DAO", "length": 60, "aa_cov": 0.8055555555555556, "hmm_cov": 1.0, "evalue": 3.4703855491918556e-20, "score": 67.88536834716797, "start": 5, "stop": 62 }, { "id": "PF13450.12", "name": "NAD_binding_8", "length": 36, "aa_cov": 0.5, "hmm_cov": 1.0, "evalue": 1.6309052580923524e-09, "score": 33.05045700073242, "start": 8, "stop": 43 }, { "id": "PF00070.33", "name": "Pyr_redox", "length": 31, "aa_cov": 0.4305555555555556, "hmm_cov": 1.0, "evalue": 1.4411684102090958e-06, "score": 23.84930419921875, "start": 6, "stop": 36 } ], "seq_stats": { "molecular_weight": 7665.8180999999995, "isoelectric_point": 8.661279106140139 }, "id": "JPCNHPFPBM_129", "locus": "GCA000292505_00089" }, { "type": "cds", "sequence": "contig_1", "start": 47577, "stop": 47852, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAAGCTATTATTTGGTAAGTTAGTTTTTAAAAAGAGCTTGTTTTTACTGAGTGGTATGAGCAGTTTAGCAGTTTTTTTAACTGCATGTGGGGCTACCAAAATATTTGATTCCTCTGTACAGCTATTAGTGTCAGATAACTTTTCCACACTTGCTGATAAATCATTTTCACAAATGTCCTATGAAGGAATCAGGAGCTTTTTTAAAAAAAGTAAGGGTTTTGATTTACCTGAAGCAGATAGTTCACAATTACAAGAAGGCAATGGCTTGTGA", "aa": "MGKLLFGKLVFKKSLFLLSGMSSLAVFLTACGATKIFDSSVQLLVSDNFSTLADKSFSQMSYEGIRSFFKKSKGFDLPEADSSQLQEGNGL", "aa_hexdigest": "a3b7697b04c14b8847366ebc0fc742ae", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9888.326699999992, "isoelectric_point": 8.818388557434083 }, "id": "JPCNHPFPBM_130", "locus": "GCA000292505_00090" }, { "type": "cds", "sequence": "contig_1", "start": 48330, "stop": 48926, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTTATAAACATTTAAGCGATGCACAAGATTCATCTGCACAAGTGACCACTTCTAAACAAACTGTTTTAAAACAACTTCAAGTTGCAAATGGTGAAAAAAAGATTAAAAAGATTAAATGGATTTCACCAAAACAAGGAAGTGATGGAGAAACTATAAACATTCAAGATCACCAATCAGGTTCTTTTTCAGATACTGAACCTAGAGCAATAACAATAGCTAATAATTTAATTGATAAAGGAGTTAATGCTATCATTCCTATTGCTGGACCACAAACGAATTTAGTGGTTACTCAAATTGCTAGAAGACAAGCCCATACTGCAGTTATTGGCGTTGATAGTGCACAGGAATTGCTAGATATTAATATTGATGCTCCAAATAAAGATAAGTTAAAAATGGGGAATAAAAAGATTATTCCCTTCTCTTCTATTAAGGCTTTGGATGTTGCTGTTGAAAGTATCTTATCAACATTAGAAAAAGGTTCCAGTCAAAATGGTTATCAGGGCTTTGGATATAACAACATAGGTACAGTAAAAAACAACTCTGTTGGGGTTAGTGAAGCAGGTTATGAATTTTTAATAGATCCTGTTTTTTGA", "aa": "MIYKHLSDAQDSSAQVTTSKQTVLKQLQVANGEKKIKKIKWISPKQGSDGETINIQDHQSGSFSDTEPRAITIANNLIDKGVNAIIPIAGPQTNLVVTQIARRQAHTAVIGVDSAQELLDINIDAPNKDKLKMGNKKIIPFSSIKALDVAVESILSTLEKGSSQNGYQGFGYNNIGTVKNNSVGVSEAGYEFLIDPVF", "aa_hexdigest": "ca5c971bb8a60020c9ff36d2bd861c13", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21363.932499999984, "isoelectric_point": 7.869736289978027 }, "id": "JPCNHPFPBM_131", "locus": "GCA000292505_00091" }, { "type": "cds", "sequence": "contig_1", "start": 48942, "stop": 49187, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGCTATGTCTTTATCAGCAAGTCTAAAAGCTAATGCAGCATCTTCATCAGATAATAAGAAAAAATTATCAGAAGTTGCTACTAAGAAAAATGAAAACGGTTCGACAAAAAATGGTAGTAATGGCATCATTGACAAATATGCCAAACTCTTAACAAAATCTAGTTCTTCAACTAGCATGAGAAACGGTAGTTCAGATAGCAATCAACAGAATTTTAAAACAACAGATAATGATGGTGATTGA", "aa": "MQAMSLSASLKANAASSSDNKKKLSEVATKKNENGSTKNGSNGIIDKYAKLLTKSSSSTSMRNGSSDSNQQNFKTTDNDGD", "aa_hexdigest": "27ab0c851a5a029a78ff812b2b7247cb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8523.170799999996, "isoelectric_point": 9.522834587097169 }, "id": "JPCNHPFPBM_132", "locus": "GCA000292505_00092" }, { "type": "cds", "sequence": "contig_1", "start": 49373, "stop": 49639, "strand": "+", "frame": 2, "gene": "ptsH", "product": "Phosphocarrier protein HPr", "db_xrefs": [ "COG:COG1925", "COG:TG", "GO:0005737", "GO:0009401", "RefSeq:WP_010869302.1", "SO:0001217", "UniParc:UPI00001328F4", "UniRef:UniRef100_P47287", "UniRef:UniRef50_P75061", "UniRef:UniRef90_P47287" ], "nt": "ATGAAAAAATTTCAAGCAGTTATTAAAGACCCAGTGGGAATTCACGCACGTCCTGCTTCTATCCTTGCAAGTGAGGCTAGTAAGTTTAAATCTGAACTTAAACTGGTAGCTCCAAGTGGTGTTGAAGGTAATATTAAATCAATCATTAACTTAATGTCTTTAGGAATTAGACATAATGACAACATTACTATCAAAGCAGATGGAGCTGATGAAGAAGAAGCTTTAGCAGCTATTAAAGCTTGTCTTGAAAAAAATAAAGTTATCTAA", "aa": "MKKFQAVIKDPVGIHARPASILASEASKFKSELKLVAPSGVEGNIKSIINLMSLGIRHNDNITIKADGADEEEALAAIKACLEKNKVI", "aa_hexdigest": "e0b02b7b8a2b84a6d8cee18d7f134960", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001328F4", "ncbi_nrp_id": "WP_010869302.1", "uniref100_id": "UniRef100_P47287", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001328F4", "RefSeq:WP_010869302.1", "UniRef:UniRef100_P47287" ] }, "ips": { "uniref100_id": "UniRef100_P47287", "uniref90_id": "UniRef90_P47287" }, "psc": { "uniref90_id": "UniRef90_P47287", "gene": "ptsH", "product": "Phosphocarrier protein HPr", "uniref50_id": "UniRef50_P75061", "cog_id": "COG1925", "cog_category": "TG", "go_ids": [ "GO:0005737", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P75061", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75061" ], "product": "Phosphocarrier protein HPr" }, "genes": [ "ptsH" ], "id": "JPCNHPFPBM_133", "locus": "GCA000292505_00093" }, { "type": "cds", "sequence": "contig_1", "start": 49837, "stop": 50160, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920RY59" ], "nt": "TTGAACGAACATTCTTTAATTGAAATTGAAGGTTTGAACAAGACCTTTGATGATGGTTATGTTTCTATAAGAGACATTAGCCTAAATATTAAAAAAGGCGAATTTATTACTATTTTAGGCCCTTCTGGTTGTGGTAAAACTACCCTGTTGAGGTTATTAGCTGGATTTGAAGATCCTACTTATGGCAAGATCAAAGTTAATGGTATTGACATTAAAGACATGGCAATCCATAAGCGTCCTTTTGCGACAGTTTTTCAAGACTATGCTTTATTTTCCCATCTAACTGTTTATAAAAACATTGCTTATGGTCTGAAGGTAATGTGA", "aa": "MNEHSLIEIEGLNKTFDDGYVSIRDISLNIKKGEFITILGPSGCGKTTLLRLLAGFEDPTYGKIKVNGIDIKDMAIHKRPFATVFQDYALFSHLTVYKNIAYGLKVM", "aa_hexdigest": "afb91b292ce699a39b0de5f268b09fee", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A920RY59", "query_cov": 0.9719626168224299, "subject_cov": 0.9541284403669725, "identity": 0.519, "score": 112.0, "evalue": 5.42e-30, "valid": false, "product": "ABC transporter domain-containing protein", "uniref50_id": "UniRef50_A0A920RY59" }, "pscc": { "uniref50_id": "UniRef50_A0A920RY59", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920RY59" ], "product": "ABC transporter domain-containing protein" }, "genes": [], "id": "JPCNHPFPBM_134", "locus": "GCA000292505_00094" }, { "type": "cds", "sequence": "contig_1", "start": 50569, "stop": 50721, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGCTGATATTAAAGCAACAAAGAACAAAGAACAAATCCAAAAATTAACTAAAGAACTTCAAACCTTAAAACAAAAATACGCTAATAAAAAAGCAATTGACAAAGAGTATGACAAATTAGTTGTAGCTTACAATAAGAAAGACTATTGA", "aa": "MKADIKATKNKEQIQKLTKELQTLKQKYANKKAIDKEYDKLVVAYNKKDY", "aa_hexdigest": "18160e4ff5543dacd31e6a7f419d6c81", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5947.898900000004, "isoelectric_point": 9.699220085144042 }, "id": "JPCNHPFPBM_135", "locus": "GCA000292505_00095" }, { "type": "cds", "sequence": "contig_1", "start": 50836, "stop": 51165, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00237D6FE8" ], "nt": "ATGGTTGGTTTAAAAGGTAAAGAAGATCGTTTGCCTGATGAATTATCAGGGGGAATGAAACAAAGAGTTGCTTTAGCACGTTCTTTAGTAGTAGAACCTGAAATTCTTTTATTAGATGAACCATTATCTGCACTTGATGCAAAGGTTAGAAAGAATTTACAAAAAGAATTACAACAGATTCATAAAAAAAGTGGATTGACTTTTATCTTAGTAACTCATGATCAAGAAGAGGCTTTAGTTTTATCAGATCGGATAGTGGTTATGAATGAGGGAAACATCTTACAAGTTGGTAATCCTGTTGATATTTATGACTCTCCTAAGACTGAATGA", "aa": "MVGLKGKEDRLPDELSGGMKQRVALARSLVVEPEILLLDEPLSALDAKVRKNLQKELQQIHKKSGLTFILVTHDQEEALVLSDRIVVMNEGNILQVGNPVDIYDSPKTE", "aa_hexdigest": "2ac62bb188addd48c6339b1c99c3a907", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00237D6FE8", "query_cov": 0.9908256880733946, "subject_cov": 0.8503937007874016, "identity": 0.574, "score": 124.0, "evalue": 1.36e-34, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI00237D6FE8" }, "pscc": { "uniref50_id": "UniRef50_UPI00237D6FE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00237D6FE8" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "JPCNHPFPBM_136", "locus": "GCA000292505_00096" }, { "type": "cds", "sequence": "contig_1", "start": 51569, "stop": 51697, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAATCTTCTTTATTATTCCAATGGCATGGATTATTGTTAGTGGATTACAAAGTGAAGATGGGGCTAGTATTAGTCAAAAATATGAACCACTTGTTAGTGGCTTAGGTTTTTTTAACAGTTTCTGA", "aa": "MTIFFIIPMAWIIVSGLQSEDGASISQKYEPLVSGLGFFNSF", "aa_hexdigest": "201ce317b84d1ed057cba098cbd0515d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4642.347599999999, "isoelectric_point": 4.136935234069824 }, "id": "JPCNHPFPBM_137", "locus": "GCA000292505_00097" }, { "type": "cds", "sequence": "contig_1", "start": 51860, "stop": 52354, "strand": "+", "frame": 2, "gene": "potB", "product": "Spermidine/putrescine transport system permease PotB-like protein", "db_xrefs": [ "COG:COG1176", "COG:E", "GO:0005886", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P47289", "UniRef:UniRef90_P47289" ], "nt": "TTGAAAACCCTACTTGATTTATTAATTGGACTTTCTTTAAACAGAGTTGGTGATAACAACTTAACTTTTGGTTCAGGATATACCTTACTTGGAACAATTTATCTGTTTACTCCTTTTATGTTTTTACCACTTTATAACCACTTCTGTGTTTTACCTAAAAACTTGTTGTTAGCTAGTCAAGATTTGGGTTATAACTGGATTTACAGCTTTGTGAAAGTAGTAATTCCTTTTTCTAAAACCGCAATGTTATCAGGAATTGCTTTAACTTTTTTCCCTGCTTTAACTTCAGTTGCAATTGCTCAGTTTTTAGATAACTCTAACCAAGCTGAAACCCTTGGTAACTACATATTTACCTTGGGTAATAATGGTTATGATAGTGCAATTGAAAGAGGCAGAGCTGCTGGAGCAATTATTATTGCTGCTTTAATTACTTTTGCAATTTACTTTACTGTTGTTTTTTTGCCTAAAATTGTCCGTATTGTTCATAACAAATGA", "aa": "MKTLLDLLIGLSLNRVGDNNLTFGSGYTLLGTIYLFTPFMFLPLYNHFCVLPKNLLLASQDLGYNWIYSFVKVVIPFSKTAMLSGIALTFFPALTSVAIAQFLDNSNQAETLGNYIFTLGNNGYDSAIERGRAAGAIIIAALITFAIYFTVVFLPKIVRIVHNK", "aa_hexdigest": "fa5e4ad6a602f21f6b589cb9d1636ce2", "start_type": "TTG", "rbs_motif": null, "genes": [ "potB" ], "truncated": "both", "pseudogene": { "start": 51560, "stop": 52378, "observations": { "mutation": [ 51695, 51830, 52352 ], "stop": [ 51695, 51830, 52352 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47289", "query_cov": 1.0, "subject_cov": 0.5754385964912281, "identity": 0.9940000000000001, "score": 317.0, "evalue": 9.05e-108, "gene_start": 1, "gene_end": 164, "reference_start": 114, "reference_end": 277, "reference_sequence": "MHIKKKYWLLLPFFLLMTIFFIIPMAWIIVSGLQSEDGASISQKYEPLVSGLGFFNSFWTSLWISIVTVIVALLFSFPFCYFLSQSKNKIFKAFVISIATVPIWSSFLIKLIGLKTLLDLLIGLSLNRVGDNNLTFGSGYTLLGTIYLFTPFMFLPLYNHFCVLPKNLLLASQDLGYNWIYSFVKVVIPFSKTAMLSGIALTFFPALTSVAIAQFLDNSNQAETLGNYIFTLGNNGYDSAIERGRAAGAIIIAALITFAIYFTVVFLPKIVRIVHNKWKQHEKAF" }, "paralog": false, "identity": 0.9669117647058824, "score": 508.0, "evalue": 1.37e-185, "description": "Internal stop codon at 51695, 51830, 52352. Nonsense mutation around 51695, 51830, 52352.", "psc": { "uniref90_id": "UniRef90_P47289", "gene": "potB", "product": "Spermidine/putrescine transport system permease protein PotB homolog", "uniref50_id": "UniRef50_P47289", "cog_id": "COG1176", "cog_category": "E", "go_ids": [ "GO:0005886", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47289", "UniRef:UniRef50_P47289", "COG:COG1176", "COG:E", "GO:0005886", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P47289", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47289" ], "product": "Spermidine/putrescine transport system permease protein PotB homolog" } }, "id": "JPCNHPFPBM_138", "locus": "GCA000292505_00098" }, { "type": "cds", "sequence": "contig_1", "start": 52362, "stop": 52766, "strand": "+", "frame": 3, "gene": "potC", "product": "Spermidine/putrescine transport system permease PotC-like protein", "db_xrefs": [ "COG:COG1177", "COG:E", "GO:0005886", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P47290", "UniRef:UniRef90_P47290" ], "nt": "ATGAAAAAGCATTTTAAGAATTTAATTAAAAACAGTTATTTCTTTCTGTTAATAACTTTAATCTATTTACCACTTTTAATAGTTGTACTTGTTAGCTTAAACGGTTCTTCTTCAAGAGGAAATATAGTGCTTGATTTTGGTAATGTTTTAAATCCTAATCCTGATTCTAAATCTGCTTATTTAAGATTAGGTGAAACTGATTTTGCAACACCACTAATAAATTCAATCATTATAGGTGTGATCACTGTTTTAGTGTCTGTTCCTATTGCTGTTATCAGTGCGTTTGCGCTTTTAAGAACAAGGAATGCTTTAAAAAAGACAATCTTTGGAATTACTAATTTTTCTTTAGCAACTCCTGATATTATTACTGCTATCTCTTTAGTGTTGTTATTTGCTAACACTTGA", "aa": "MKKHFKNLIKNSYFFLLITLIYLPLLIVVLVSLNGSSSRGNIVLDFGNVLNPNPDSKSAYLRLGETDFATPLINSIIIGVITVLVSVPIAVISAFALLRTRNALKKTIFGITNFSLATPDIITAISLVLLFANT", "aa_hexdigest": "d5eb35154948182a746979f9cd434e24", "start_type": "ATG", "rbs_motif": null, "genes": [ "potC" ], "truncated": "3-prime", "pseudogene": { "start": 52362, "stop": 53069, "observations": { "mutation": [ 52764 ], "stop": [ 52764 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47290", "query_cov": 1.0, "subject_cov": 0.47183098591549294, "identity": 1.0, "score": 238.0, "evalue": 3.68e-77, "gene_start": 1, "gene_end": 134, "reference_start": 1, "reference_end": 134, "reference_sequence": "MKKHFKNLIKNSYFFLLITLIYLPLLIVVLVSLNGSSSRGNIVLDFGNVLNPNPDSKSAYLRLGETDFATPLINSIIIGVITVLVSVPIAVISAFALLRTRNALKKTIFGITNFSLATPDIITAISLVLLFANTWLSFNQQLGFFTIITSHISFSVPYALILIYPKIQKLNPNLILASQDLGYSPLKTFFHITLPYLMPSIFSAVLVVFATSFDDYVITSLVQGSVKTIATELYSFRKGIKAWAIAFGSILILISVLGVCLITLQKYLREKRKEIIKIRQWKNS" }, "paralog": false, "identity": 0.9957446808510638, "score": 445.0, "evalue": 3.82e-161, "description": "Internal stop codon at 52764. Nonsense mutation around 52764.", "psc": { "uniref90_id": "UniRef90_P47290", "gene": "potC", "product": "Spermidine/putrescine transport system permease protein PotC homolog", "uniref50_id": "UniRef50_P47290", "cog_id": "COG1177", "cog_category": "E", "go_ids": [ "GO:0005886", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47290", "UniRef:UniRef50_P47290", "COG:COG1177", "COG:E", "GO:0005886", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P47290", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47290" ], "product": "Spermidine/putrescine transport system permease protein PotC homolog" } }, "id": "JPCNHPFPBM_139", "locus": "GCA000292505_00099" }, { "type": "cds", "sequence": "contig_1", "start": 52827, "stop": 53204, "strand": "+", "frame": 3, "gene": "potC", "product": "Spermidine/putrescine transport system permease PotC-like protein", "db_xrefs": [ "COG:COG1177", "COG:E", "GO:0005886", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P47290", "UniRef:UniRef90_P47290" ], "nt": "GTGCCTTATGCATTGATTTTGATTTACCCTAAAATTCAAAAATTGAATCCTAATTTAATTCTTGCTTCTCAAGATTTAGGCTATTCGCCTTTAAAAACTTTTTTCCATATTACTCTACCTTATCTAATGCCAAGTATTTTTTCAGCAGTACTAGTAGTATTTGCAACTAGTTTTGATGATTATGTAATTACCTCTTTAGTACAAGGATCAGTAAAAACTATAGCAACTGAACTCTATTCATTTAGAAAAGGAATTAAAGCATGGGCAATCGCCTTTGGGTCTATTCTCATATTGATTAGTGTCTTAGGAGTCTGTTTAATAACCCTGCAAAAGTATTTAAGGGAAAAAAGAAAGGAAATAATCAAAATAAGACAATGA", "aa": "MPYALILIYPKIQKLNPNLILASQDLGYSPLKTFFHITLPYLMPSIFSAVLVVFATSFDDYVITSLVQGSVKTIATELYSFRKGIKAWAIAFGSILILISVLGVCLITLQKYLREKRKEIIKIRQ", "aa_hexdigest": "e75e66aadada292443606bf52a3766c4", "start_type": "GTG", "rbs_motif": null, "genes": [ "potC" ], "truncated": "both", "pseudogene": { "start": 52527, "stop": 53216, "observations": { "mutation": [ 52764, 53202 ], "stop": [ 52764, 53202 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47290", "query_cov": 0.992, "subject_cov": 0.43661971830985913, "identity": 1.0, "score": 229.0, "evalue": 1.58e-73, "gene_start": 2, "gene_end": 125, "reference_start": 157, "reference_end": 280, "reference_sequence": "MKKHFKNLIKNSYFFLLITLIYLPLLIVVLVSLNGSSSRGNIVLDFGNVLNPNPDSKSAYLRLGETDFATPLINSIIIGVITVLVSVPIAVISAFALLRTRNALKKTIFGITNFSLATPDIITAISLVLLFANTWLSFNQQLGFFTIITSHISFSVPYALILIYPKIQKLNPNLILASQDLGYSPLKTFFHITLPYLMPSIFSAVLVVFATSFDDYVITSLVQGSVKTIATELYSFRKGIKAWAIAFGSILILISVLGVCLITLQKYLREKRKEIIKIRQWKNS" }, "paralog": false, "identity": 0.9912663755458515, "score": 427.0, "evalue": 3.83e-154, "description": "Internal stop codon at 52764, 53202. Nonsense mutation around 52764, 53202.", "psc": { "uniref90_id": "UniRef90_P47290", "gene": "potC", "product": "Spermidine/putrescine transport system permease protein PotC homolog", "uniref50_id": "UniRef50_P47290", "cog_id": "COG1177", "cog_category": "E", "go_ids": [ "GO:0005886", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47290", "UniRef:UniRef50_P47290", "COG:COG1177", "COG:E", "GO:0005886", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P47290", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47290" ], "product": "Spermidine/putrescine transport system permease protein PotC homolog" } }, "id": "JPCNHPFPBM_140", "locus": "GCA000292505_00100" }, { "type": "cds", "sequence": "contig_1", "start": 53201, "stop": 53485, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACAGTTAAAATATTGCTTTTTCTCACTTTTTGTTAGTCTCTCATCAATATTGAGTAGTTGTGGTTCAACAACATTTGTACTAGCTAACTTTGAATCTTATATTTCGCCCTTATTGCTAGAAAGAGTACAAGAAAAACATCCCTTAACTTTCTTGACTTATCCTAGTAATGAAAAACTAATTAATGGTTTTGCTAACAACACTTATTCAGTAGCAGTAGCATCTACTTATGCAGTTAGTGAATTGATAGAAAGGGATCTATTATCACCAATAGATTGA", "aa": "MKKQLKYCFFSLFVSLSSILSSCGSTTFVLANFESYISPLLLERVQEKHPLTFLTYPSNEKLINGFANNTYSVAVASTYAVSELIERDLLSPID", "aa_hexdigest": "eac83ea179219eaf3c77ac5f95e39326", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10552.020999999988, "isoelectric_point": 5.723197746276856 }, "id": "JPCNHPFPBM_141", "locus": "GCA000292505_00101" }, { "type": "cds", "sequence": "contig_1", "start": 53732, "stop": 54565, "strand": "+", "frame": 2, "gene": "potD", "product": "putative lipoprotein MG045", "db_xrefs": [ "COG:COG0687", "COG:E", "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75056", "UniRef:UniRef90_P47291" ], "nt": "GTGAAACACAAAGATCGCTTTAATGATAATAGGTTAGTTTTCATTGATGATGCTAGAACGATCTTTTCACTTGCTAACATCGTTAATACTAACAACAATTCAACTGATGTTAATCCAAAGGAAAATGGAATTGGTTATTTCACTAATGTCTATGAAAGCTTTCAAAGACTTGGATTAACAAAATCTAATTTAGATAGTATCTTTGTTAATTCTGATTCCAATATTGTGATCAATGAATTGGCAAGTGGTAAAAGACAAGGAGGAATTGTTTACAATGGTGATGCAGTGTATGCTGCATTGGGCGGTGATTTACGTGATGAATTGAGTGAAGAACAGATTCCTGATGGGAACAACTTTCACATTGTGCAACCCAAAATTTCCCCAGTTGCTTTAGATCTTTTGGTTATCAATAAACAACAATCTAATTTTCAAAAAGAAGCACATGAGATCATTTTTGATCTTGCTTTGGATGGTGCTGATCAAACTAAAGAACAGTTAATTAAAACTGATGAAGAATTGGGTACTGATGATGAAGACTTTTACTTAAAAGGAGCGATGCAAAACTTTAGTTATGTGAACTATGTTTCACCATTAAAAGTAATATCTGATCCAAGTACTGGAATAGTCAATTCCAAAAAGAATAATGCTGAAATGAAAAGTAAACAAATGTCAACTGATCAAATGACTAGTGAAAAAGAATTTGATTATTACACTGAAACACTTAAAGCATTATTAGAGAAAAAAGATAGTGAAGAATTAAATGAAAATGAAAAAAAACTAGTTGAAACCATTAAGAAAGCTTACACTATTGAAAAAGATAGTTCAATTCAGTGA", "aa": "MKHKDRFNDNRLVFIDDARTIFSLANIVNTNNNSTDVNPKENGIGYFTNVYESFQRLGLTKSNLDSIFVNSDSNIVINELASGKRQGGIVYNGDAVYAALGGDLRDELSEEQIPDGNNFHIVQPKISPVALDLLVINKQQSNFQKEAHEIIFDLALDGADQTKEQLIKTDEELGTDDEDFYLKGAMQNFSYVNYVSPLKVISDPSTGIVNSKKNNAEMKSKQMSTDQMTSEKEFDYYTETLKALLEKKDSEELNENEKKLVETIKKAYTIEKDSSIQ", "aa_hexdigest": "05067e60578bcce45a14529f0a2a21fd", "start_type": "GTG", "rbs_motif": null, "genes": [ "potD" ], "truncated": "both", "pseudogene": { "start": 53432, "stop": 54649, "observations": { "mutation": [ 53483, 53624, 53708, 54563, 54623 ], "stop": [ 53483, 53624, 53708, 54563, 54623 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47291", "query_cov": 1.0, "subject_cov": 0.5734989648033126, "identity": 0.971, "score": 510.0, "evalue": 5.13e-179, "gene_start": 1, "gene_end": 277, "reference_start": 178, "reference_end": 454, "reference_sequence": "MKKQLKYCFFSLFVSLSSILSSCGSTTFVLANFESYISPLLLERVQEKHPLTFLTYPSNEKLINGFANNTYSVAVASTYAVSELIERDLLSPIDWSQFNLKKSSSSSDKVNNASDAKDLFIDSIKEISQQTKDSKNNELLHWAVPYFLQNLVFVYRGEKISELEQENVSWTDVIKAIVKHKDRFNDNRLVFIDDARTIFSLANIVNTNNNSADVNPKEDGIGYFTNVYESFQRLGLTKSNLDSIFVNSDSNIVINELASGRRQGGIVYNGDAVYAALGGDLRDELSEEQIPDGNNFHIVQPKISPVALDLLVINKQQSNFQKEAHEIIFDLALDGADQTKEQLIKTDEELGTDDEDFYLKGAMQNFSYVNYVSPLKVISDPSTGIVSSKKNNAEMKSKQMSTDQMTSEKEFDYYTETLKALLEKEDSAELNENEKKLVETIKKAYTIEKDSSIRWNQLVEKPISPLQRSNLSLSWLDFKLHWW" }, "paralog": false, "identity": 0.9703703703703703, "score": 760.0, "evalue": 4.64e-280, "description": "Internal stop codon at 53483, 53624, 53708, 54563, 54623. Nonsense mutation around 53483, 53624, 53708, 54563, 54623.", "psc": { "uniref90_id": "UniRef90_P47291", "gene": "PotD", "product": "Uncharacterized lipoprotein MG045", "uniref50_id": "UniRef50_P75056", "cog_id": "COG0687", "cog_category": "E", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47291", "UniRef:UniRef50_P75056", "COG:COG0687", "COG:E", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75056", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75056" ], "product": "Uncharacterized lipoprotein MG045 homolog" } }, "id": "JPCNHPFPBM_142", "locus": "GCA000292505_00102" }, { "type": "cds", "sequence": "contig_1", "start": 54654, "stop": 55268, "strand": "+", "frame": 3, "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "db_xrefs": [ "COG:COG0533", "COG:J", "EC:2.3.1.234", "GO:0002949", "GO:0005506", "GO:0005737", "GO:0061711", "SO:0001217", "UniRef:UniRef50_P75055", "UniRef:UniRef90_P47292" ], "nt": "ATGGAACAGCCGTTGTGTGTTTTAGGGATTGAAACAACCTGTGATGATACAGGTCTTAGTATTGTCATTGATCAAAAAATCAAGAGTAACATTGTTATCTCTTCTGCTAACTTACATGTAAAAACAGGAGGAGTTGTACCTGAAATTGCAGCACGATGCCACGAACAAAATCTCTTTAAAGCAATAAGAGATTTAAATTTTGAGATAAGAGATTTATCTCACATTGCTTATGCATGTAATCCTGGGTTAGCAGGATGTTTACATGTGGGAGCCACTTTTGCTAGAAGCTTAAGTTTCTTATTAGACAAACCATTGTTACCCATCAACCATCTTTATGCGCATATCTTTTCTTGTTTAATTGATCAAGATTTAAATAAGCTGCAATTACCAGCATTAGGCCTTGTAATTTCAGGTGGTCATACTGCTATTTATCTAGTTAAATCATTTTATGAACTTGAACTAATTGCTGAAACTAGTGATGATGCAATTGGTGAAGTTTATGACAAGATAGGCAGAGCAATGGGCTTTGATTATCCTGCTGGTAGTAAAATTGATAGTCTTTTTAATAAAGAATTAGTTAAACCTCACTATTTCTTTAAACCTTCTACTAAGTGA", "aa": "MEQPLCVLGIETTCDDTGLSIVIDQKIKSNIVISSANLHVKTGGVVPEIAARCHEQNLFKAIRDLNFEIRDLSHIAYACNPGLAGCLHVGATFARSLSFLLDKPLLPINHLYAHIFSCLIDQDLNKLQLPALGLVISGGHTAIYLVKSFYELELIAETSDDAIGEVYDKIGRAMGFDYPAGSKIDSLFNKELVKPHYFFKPSTK", "aa_hexdigest": "276fba995d1708c809dbb1bf246e6bd0", "start_type": "ATG", "rbs_motif": null, "genes": [ "tsaD" ], "truncated": "3-prime", "pseudogene": { "start": 54654, "stop": 55571, "observations": { "mutation": [ 55266 ], "stop": [ 55266 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47292", "query_cov": 1.0, "subject_cov": 0.6476190476190476, "identity": 1.0, "score": 416.0, "evalue": 1.41e-145, "gene_start": 1, "gene_end": 204, "reference_start": 1, "reference_end": 204, "reference_sequence": "MEQPLCVLGIETTCDDTGLSIVIDQKIKSNIVISSANLHVKTGGVVPEIAARCHEQNLFKAIRDLNFEIRDLSHIAYACNPGLAGCLHVGATFARSLSFLLDKPLLPINHLYAHIFSCLIDQDLNKLQLPALGLVISGGHTAIYLVKSFYELELIAETSDDAIGEVYDKIGRAMGFDYPAGSKIDSLFNKELVKPHYFFKPSTKWTKFSYSGLKSQCLNKIKQISANKTRIDWSELASNFQATIIDHYIDHVKNAIKKFAPKMLLVGGGVSANSYLSNRISTLNLPFLIADSKYTSDNGAMIGFYASLLINGDKN" }, "paralog": false, "identity": 0.9967213114754099, "score": 608.0, "evalue": 1.01e-223, "description": "Internal stop codon at 55266. Nonsense mutation around 55266.", "psc": { "uniref90_id": "UniRef90_P47292", "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_P75055", "cog_id": "COG0533", "cog_category": "J", "go_ids": [ "GO:0002949", "GO:0005506", "GO:0005737", "GO:0061711" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47292", "EC:2.3.1.234", "UniRef:UniRef50_P75055", "COG:COG0533", "COG:J", "GO:0002949", "GO:0005506", "GO:0005737", "GO:0061711" ] }, "pscc": { "uniref50_id": "UniRef50_P75055", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75055" ], "product": "tRNA N6-adenosine threonylcarbamoyltransferase" } }, "id": "JPCNHPFPBM_143", "locus": "GCA000292505_00103" }, { "type": "cds", "sequence": "contig_1", "start": 55440, "stop": 55601, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGTTAGTAGGAGGTGGAGTTAGTGCCAATTCTTATCTATCTAACAGAATTAGTACATTAAATTTACCCTTTTTAATTGCTGATAGCAAATACACCAGTGATAATGGAGCAATGATTGGTTTTTATGCATCACTTTTAATTAATGGCGATAAGAATTAA", "aa": "MLLVGGGVSANSYLSNRISTLNLPFLIADSKYTSDNGAMIGFYASLLINGDKN", "aa_hexdigest": "6946ae4a63279b6a7e7fea716897fbf0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5598.319300000001, "isoelectric_point": 5.793621253967285 }, "id": "JPCNHPFPBM_144", "locus": "GCA000292505_00104" }, { "type": "cds", "sequence": "contig_1", "start": 55585, "stop": 55815, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00438.25" ], "nt": "ATGGCGATAAGAATTAAAAGTACAAGAGTTGGTAGATTTGTTTCTGAATCAGTGGGATTAGGTCATCCTGATAAAATTTGTGATCAGATTGCAGATAGTATCTTAGACCAATGTTTACTACAGAGTAAAACTAGTCATGTAGCATGTGAAGTCTTTGCTTCTAAAAACCTTATTTTAATAGGTGGTGAGATTTCAACAAGTGGCTATGTTGATGTTGTTCAAACTGCTTGA", "aa": "MAIRIKSTRVGRFVSESVGLGHPDKICDQIADSILDQCLLQSKTSHVACEVFASKNLILIGGEISTSGYVDVVQTA", "aa_hexdigest": "be4374b706773c2248f95f85620d3c9e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00438.25", "name": "S-AdoMet_synt_N", "length": 63, "aa_cov": 0.8289473684210527, "hmm_cov": 1.0, "evalue": 5.227071740405412e-26, "score": 86.24707794189453, "start": 13, "stop": 75 } ], "seq_stats": { "molecular_weight": 8121.262299999996, "isoelectric_point": 6.01432704925537 }, "id": "JPCNHPFPBM_145", "locus": "GCA000292505_00105" }, { "type": "cds", "sequence": "contig_1", "start": 55960, "stop": 56475, "strand": "+", "frame": 1, "gene": "metK", "product": "methionine adenosyltransferase", "db_xrefs": [ "COG:COG0192", "COG:H", "EC:2.5.1.6", "GO:0000287", "GO:0004478", "GO:0005524", "GO:0005829", "GO:0006556", "GO:0006730", "KEGG:K00789", "SO:0001217", "UniRef:UniRef50_P47293", "UniRef:UniRef90_P47293" ], "nt": "GTGAATGAAACAAAGCAACTAATGCCTTTAGGAGTTTTACTAGCACACTCGTTTTTAAAACAAGCAGAAAAACTAACAAAACAATTTGATTTTTTAAAAAATGATATGAAAAGTCAAGTGGTTTTAAACTACAGTTTAAACCAAGTTGAATGTGAAGAAGTTTTACTATCAATTCAACACACTAATGCTATTAGTTTAACAGAATTGAGAAAAGTGATTGAAAATAATGTAATTCTACCTGTTTTAAACCAATATGGTTTTCAAGATAAAAAGCCAACTTGTTTAGTGAATCCTGGTGGTTCTTTTGTTTTAGGTGGACCTATGGCAGATACTGGACTAACTGGTAGAAAAATCATTGTTGACACCTATGGTCCATATGCTCACCATGGTGGTGGTAGCTTTAGTGGCAAAGATCCTAGTAAGGTGGATAGAACAGGTGCTTATTTTGCACGTTTTATCGCAAAACATATTGTAAGTTTAGGCTGGGCCAGTGAGTGTGAAGTCAGTATTAGCTGA", "aa": "MNETKQLMPLGVLLAHSFLKQAEKLTKQFDFLKNDMKSQVVLNYSLNQVECEEVLLSIQHTNAISLTELRKVIENNVILPVLNQYGFQDKKPTCLVNPGGSFVLGGPMADTGLTGRKIIVDTYGPYAHHGGGSFSGKDPSKVDRTGAYFARFIAKHIVSLGWASECEVSIS", "aa_hexdigest": "7cbf5654cc2b87a4fbb0719aa3d9d84b", "start_type": "GTG", "rbs_motif": null, "genes": [ "metK" ], "truncated": "both", "pseudogene": { "start": 55660, "stop": 56736, "observations": { "mutation": [ 55813, 56473, 56581, 56680 ], "stop": [ 55813, 56473, 56581, 56680 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47293", "query_cov": 0.9941520467836257, "subject_cov": 0.44386422976501305, "identity": 1.0, "score": 343.0, "evalue": 2.92e-116, "gene_start": 2, "gene_end": 171, "reference_start": 127, "reference_end": 296, "reference_sequence": "MAIRIKSTRVGRFVSESVGLGHPDKICDQIADSILDQCLLQSKTSHVACEVFASKNLILIGGEISTSGYVDVVQTAWRILRNLGYNETDFSFLSCINNQSLEINQAVLKNNEINAGDQGITVGYAVNETKQLMPLGVLLAHSFLKQAEKLTKQFDFLKNDMKSQVVLNYSLNQVECEEVLLSIQHTNAISLTELRKVIENNVILPVLNQYGFQDKKPTCLVNPGGSFVLGGPMADTGLTGRKIIVDTYGPYAHHGGGSFSGKDPSKVDRTGAYFARFIAKHIVSLGWASECEVSISWVFSKPNPQSITVKCFNTNIQYDEVLINRVVNNYFNWSITKIIDKLKLLDFVKYSDYAVYGHFGNDLSPWEQPTELDKLECLIKNFH" }, "paralog": false, "identity": 0.9888268156424581, "score": 704.0, "evalue": 2e-261, "description": "Internal stop codon at 55813, 56473, 56581, 56680. Nonsense mutation around 55813, 56473, 56581, 56680.", "psc": { "uniref90_id": "UniRef90_P47293", "gene": "metK", "product": "methionine adenosyltransferase", "ec_ids": [ "2.5.1.6" ], "uniref50_id": "UniRef50_P47293", "cog_id": "COG0192", "cog_category": "H", "kegg_orthology_id": "K00789", "go_ids": [ "GO:0000287", "GO:0004478", "GO:0005524", "GO:0005829", "GO:0006556", "GO:0006730" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47293", "EC:2.5.1.6", "UniRef:UniRef50_P47293", "COG:COG0192", "COG:H", "KEGG:K00789", "GO:0000287", "GO:0004478", "GO:0005524", "GO:0005829", "GO:0006556", "GO:0006730" ] }, "pscc": { "uniref50_id": "UniRef50_P47293", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47293" ], "product": "S-adenosylmethionine synthase" } }, "id": "JPCNHPFPBM_146", "locus": "GCA000292505_00106" }, { "type": "cds", "sequence": "contig_1", "start": 56712, "stop": 56969, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_009885710.1", "SO:0001217", "UniParc:UPI0001B41721", "UniRef:UniRef100_UPI0001B41721", "UniRef:UniRef50_UPI0001B41721", "UniRef:UniRef90_UPI0001B41721" ], "nt": "ATGCTTAATCAAAAATTTCCATTAGATCCTAATAAAAAAAAGGAACAACAAGGCGTAGTAAAACCAAATCTACCGGTTGTTAAAGAAAAGAAAAAACAGCCATTTAAAAAACCAAATTGGAGTGAATTTAAGCTGTTCAACTTTTTTAAAAAGCACACTTATTTTTGCTTGGTTGTATTAGCTTTTCTTATCTTGATTATTTTACTTAGCAGCTTATTTGCTATTCCACTTAGCCAAATACCTTCAAGTACTGTTTAA", "aa": "MLNQKFPLDPNKKKEQQGVVKPNLPVVKEKKKQPFKKPNWSEFKLFNFFKKHTYFCLVVLAFLILIILLSSLFAIPLSQIPSSTV", "aa_hexdigest": "b41c1537ab0f3685e9bf9b4b5b478b4b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001B41721", "ncbi_nrp_id": "WP_009885710.1", "uniref100_id": "UniRef100_UPI0001B41721", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001B41721", "RefSeq:WP_009885710.1", "UniRef:UniRef100_UPI0001B41721" ] }, "ips": { "uniref100_id": "UniRef100_UPI0001B41721", "uniref90_id": "UniRef90_UPI0001B41721" }, "psc": { "uniref90_id": "UniRef90_UPI0001B41721", "uniref50_id": "UniRef50_UPI0001B41721" }, "pscc": { "uniref50_id": "UniRef50_UPI0001B41721", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B41721" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9889.837899999991, "isoelectric_point": 10.067270088195798 }, "id": "JPCNHPFPBM_147", "locus": "GCA000292505_00107" }, { "type": "cds", "sequence": "contig_1", "start": 57053, "stop": 57172, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02978.25" ], "nt": "ATGACTAGAGAAGAAAGGAGACATCCCAAATTAATTAATCGTGATCCTAATAGAAAACAGCGCATCATAAAAGGATCAGGGAGAAAAATGGATGAGTTAAACAAACTGATGAAGGAATGA", "aa": "MTREERRHPKLINRDPNRKQRIIKGSGRKMDELNKLMKE", "aa_hexdigest": "ad263c070bd32515b7247a288903f725", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02978.25", "name": "SRP_SPB", "length": 39, "aa_cov": 1.0, "hmm_cov": 0.9487179487179487, "evalue": 7.976601147605881e-12, "score": 40.56576919555664, "start": 1, "stop": 39 } ], "seq_stats": { "molecular_weight": 4803.603900000001, "isoelectric_point": 10.963251686096193 }, "id": "JPCNHPFPBM_148", "locus": "GCA000292505_00108" }, { "type": "cds", "sequence": "contig_1", "start": 57191, "stop": 57913, "strand": "-", "frame": 3, "gene": "ffh", "product": "signal recognition particle protein", "db_xrefs": [ "COG:COG0541", "COG:U", "EC:3.6.5.4", "GO:0003924", "GO:0005048", "GO:0005525", "GO:0006614", "GO:0008312", "GO:0009306", "GO:0016887", "GO:0048500", "GO:0048501", "KEGG:K03106", "SO:0001217", "UniRef:UniRef50_P75054", "UniRef:UniRef90_P47294" ], "nt": "ATGTTAGTAGGCTTGGACATCTACAGACCCGCTGCCATTGAACAACTTGAAACGCTTTCACAACAAACTAACAGCGTATTTTTTGCACAAGGCACTCAACCAGTTGCTAAAACAACAAAAGCAGCACTCAGTGCTTTTAAAACTGCAAAATGTCAAGCAATCATTTGTGATACCGCTGGTAGATTACAAACAAATGAAACATTAATGGATGAATTGGTAAGTGTTAAAAATGAATTAAATCCTGATGAAATTATCATGGTAGTAGATGGATTAAGCGGTCAGGAAATTATCAATGTTGCTCAAACGTTCCACAAACGTTTAAAACTAACTGGATTTATTATCAGTAAATTAGACAGTGATGCTAGAGCAGGAGCTGCACTTTCATTAGCTTCACTTTTACAAGTACCCATTAAATTAATTGGTGTTTCTGAAAAATTAGATGGATTGGAACAATTTCATCCTGAAAGGATAGCCAATCGGATCTTAGGTTTGGGTGATGTAATGAGTTTAGTTGAAAAAGCTGAACAAGTTTTTGATAAAAAAGATTTAACTAAAACCATCAGCAAGATGTTTTTGGGAAAAATGGATTTAGAAGATCTTTTGATCTACATGCAACAAATGCACAAAATGGGAAGTGTCAGTTCACTGATAAAAATGTTGCCTGCTAACTTTTCTGTATCAGAAGAAAATGCTGAATTAATTGAAAACAAAATTGAACTATGA", "aa": "MLVGLDIYRPAAIEQLETLSQQTNSVFFAQGTQPVAKTTKAALSAFKTAKCQAIICDTAGRLQTNETLMDELVSVKNELNPDEIIMVVDGLSGQEIINVAQTFHKRLKLTGFIISKLDSDARAGAALSLASLLQVPIKLIGVSEKLDGLEQFHPERIANRILGLGDVMSLVEKAEQVFDKKDLTKTISKMFLGKMDLEDLLIYMQQMHKMGSVSSLIKMLPANFSVSEENAELIENKIEL", "aa_hexdigest": "3b190a50b43f95df42a1b232bfb5d714", "start_type": "ATG", "rbs_motif": null, "genes": [ "ffh" ], "truncated": "both", "pseudogene": { "start": 56966, "stop": 58213, "observations": { "mutation": [ 57055, 57193, 57943 ], "stop": [ 57055, 57193, 57943 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47294", "query_cov": 1.0, "subject_cov": 0.5381165919282511, "identity": 0.996, "score": 414.0, "evalue": 4.44e-142, "gene_start": 1, "gene_end": 240, "reference_start": 132, "reference_end": 371, "reference_sequence": "MFKAMLSSIVMRTMQKKINAQTITEKDVELVLKEIRIALLDADVNLLVVKNFIKAIRDKTVGQTIEPGQDLQKSLLKTIKTELINILSQPNQELNEKRPLKIMMVGLQGSGKTTTCGKLAYWLEKKYKQKTMLVGLDIYRPAAIEQLETLSQQTNSVFFAQGTQPVAKTTKAALSAFKTAKCQTIICDTAGRLQTNETLMDELVSVKNELNPDEIIMVVDGLSGQEIINVAQTFHKRLKLTGFIISKLDSDARAGAALSLASLLQVPIKLIGVSEKLDGLEQFHPERIANRILGLGDVMSLVEKAEQVFDKKDLTKTISKMFLGKMDLEDLLIYMQQMHKMGSVSSLIKMLPANFSVSEENAELIENKIELWKVLINSMTREERRHPKLINRDPNRKQRIIKGSGRKMDELNKLMKEWNKMQLKATEMGKLLKTGSNPFGGFGQFF" }, "paralog": false, "identity": 0.9903614457831326, "score": 750.0, "evalue": 2.47e-277, "description": "Internal stop codon at 57055, 57193, 57943. Nonsense mutation around 57055, 57193, 57943.", "psc": { "uniref90_id": "UniRef90_P47294", "gene": "ffh", "product": "signal recognition particle protein", "ec_ids": [ "3.6.5.4" ], "uniref50_id": "UniRef50_P75054", "cog_id": "COG0541", "cog_category": "U", "kegg_orthology_id": "K03106", "go_ids": [ "GO:0003924", "GO:0005048", "GO:0005525", "GO:0006614", "GO:0008312", "GO:0009306", "GO:0016887", "GO:0048500", "GO:0048501" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47294", "EC:3.6.5.4", "UniRef:UniRef50_P75054", "COG:COG0541", "COG:U", "KEGG:K03106", "GO:0003924", "GO:0005048", "GO:0005525", "GO:0006614", "GO:0008312", "GO:0009306", "GO:0016887", "GO:0048500", "GO:0048501" ] }, "pscc": { "uniref50_id": "UniRef50_P75054", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75054" ], "product": "Signal recognition particle protein" } }, "id": "JPCNHPFPBM_149", "locus": "GCA000292505_00109" }, { "type": "cds", "sequence": "contig_1", "start": 57941, "stop": 58276, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00448.28", "PFAM:PF02881.24" ], "nt": "ATGCGCACGATGCAAAAAAAAATTAACGCTCAAACGATCACTGAGAAAGATGTAGAGTTAGTTCTAAAAGAGATTAGAATTGCATTGCTTGATGCTGATGTTAACCTGCTTGTTGTTAAAAATTTCATCAAAGCAATTAGAGATAAAACAGTAGGACAAACCATTGAACCTGGTCAAGATTTGCAAAAGTCTCTATTAAAAACAATCAAAACAGAACTAATTAATATCTTAAGCCAACCCAACCAAGAACTAAATGAAAAAAGACCTTTAAAAATAATGATGGTTGGTTTACAAGGATCAGGTAAAACAACAACTTGTGGCAAACTAGCTTATTGA", "aa": "MRTMQKKINAQTITEKDVELVLKEIRIALLDADVNLLVVKNFIKAIRDKTVGQTIEPGQDLQKSLLKTIKTELINILSQPNQELNEKRPLKIMMVGLQGSGKTTTCGKLAY", "aa_hexdigest": "c5de0bd3bf8dd932b62cf6bb17c685b0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00448.28", "name": "SRP54", "length": 20, "aa_cov": 0.18018018018018017, "hmm_cov": 1.0, "evalue": 1.4668899013495382e-08, "score": 29.639175415039062, "start": 91, "stop": 110 }, { "id": "PF02881.24", "name": "SRP54_N", "length": 58, "aa_cov": 0.5135135135135135, "hmm_cov": 1.0, "evalue": 1.0731724806065492e-15, "score": 52.79533767700195, "start": 9, "stop": 65 } ], "seq_stats": { "molecular_weight": 12461.65679999999, "isoelectric_point": 9.58872127532959 }, "id": "JPCNHPFPBM_150", "locus": "GCA000292505_00110" }, { "type": "cds", "sequence": "contig_1", "start": 58281, "stop": 58688, "strand": "+", "frame": 3, "gene": null, "product": "Uridine phosphorylase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9D1M2H1" ], "nt": "ATGCTTGATAACATTGCTTTGAACATGATTTTTAATTATTTATTATTAAATAATGTTTTAATAAAACAATATTGCAATATGACCCCACATATAAGTGCTAAGAAAGATGACATTAGCAAAGTTGTTTTAATGCCAGGTGATCCATTGAGAGCTAAATGGATAGCTGAGCAATTCTTAGATCAAGCTAAATTAGTCAATGAAGTGAGGGGAATGTTTGCTTATACTGGGCAGTATAAATCTAAAACAGTTACAGTAATGGGCCATGGAATGGGGATCCCTTCTATTGGAATTTATTCATATGAGTTGATGAATTTTTATGAGGTTGAAACTATCATTAGAATCGGAAGTTGTGGTGCTTTAGCACCGCAATTAAAATTAAAAGATCTTGTTATTGCTTCAAAAGCATGA", "aa": "MLDNIALNMIFNYLLLNNVLIKQYCNMTPHISAKKDDISKVVLMPGDPLRAKWIAEQFLDQAKLVNEVRGMFAYTGQYKSKTVTVMGHGMGIPSIGIYSYELMNFYEVETIIRIGSCGALAPQLKLKDLVIASKA", "aa_hexdigest": "5e6b0e9cbc242a2599f07e5f94feb7ac", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001FEB9C95", "query_cov": 0.8074074074074075, "subject_cov": 0.8449612403100775, "identity": 0.624, "score": 143.0, "evalue": 1.4e-41, "valid": false, "uniref50_id": "UniRef50_A0A9D1M2H1" }, "pscc": { "uniref50_id": "UniRef50_A0A9D1M2H1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9D1M2H1" ], "product": "Uridine phosphorylase" }, "genes": [], "id": "JPCNHPFPBM_151", "locus": "GCA000292505_00111" }, { "type": "cds", "sequence": "contig_1", "start": 58713, "stop": 59075, "strand": "+", "frame": 3, "gene": "deoD", "product": "purine-nucleoside phosphorylase", "db_xrefs": [ "COG:COG0813", "COG:F", "EC:2.4.2.1", "GO:0004731", "GO:0005829", "GO:0006139", "GO:0006152", "KEGG:K03784", "SO:0001217", "UniRef:UniRef50_P47295", "UniRef:UniRef90_P47295" ], "nt": "ATGGGTGTTGAAATTCCAGAAGATAAGATCTTATTTGCAACAAGTTCTTTAGTGGAATTAGCAAAAGAAACTGCGATTAAGAACAAGCTTGATTTTCATGAAGGATTAGTATTTTGTGAGGATGCTTTTTATCAAACTAGAAAAGATGTAATTAGTCTTGCTAAAGAAAAAAATAGTTTAGCAGTTGAAATGGAAGCACATGCACTTTATGCTAATGCAATCCTGTTGAAGAAAAAAGCACTTACACTCTTAACAGTATCTGATTCTCTAGTAACTCATGAAGCACTTAGTTCTGAATTAAGACAAAAGTCATTTAAGCAAATGGCTTTATTAGCACTTGAAATGACTCAAAAACTAATCTAA", "aa": "MGVEIPEDKILFATSSLVELAKETAIKNKLDFHEGLVFCEDAFYQTRKDVISLAKEKNSLAVEMEAHALYANAILLKKKALTLLTVSDSLVTHEALSSELRQKSFKQMALLALEMTQKLI", "aa_hexdigest": "b5531ca3f8d05936b458016ffb9425be", "start_type": "ATG", "rbs_motif": null, "genes": [ "deoD" ], "truncated": "5-prime", "pseudogene": { "start": 58413, "stop": 59075, "observations": { "mutation": [ 58686 ], "stop": [ 58686 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47295", "query_cov": 1.0, "subject_cov": 0.5042016806722689, "identity": 1.0, "score": 221.0, "evalue": 2.33e-71, "gene_start": 1, "gene_end": 120, "reference_start": 119, "reference_end": 238, "reference_sequence": "MTPHISAKKDDISKVVLMPGDPLRAKWIAEQFLDQAKLVNEVRGMFAYTGQYKSKTVTVMGHGMGIPSIGIYSYELMNFYEVETIIRIGSCGALAPQLKLKDLVIASKAWSESIYAKDMGVEIPEDKILFATSSLVELAKETAIKNKLDFHEGLVFCEDAFYQTRKDVISLAKEKNSLAVEMEAHALYANAILLKKKALTLLTVSDSLVTHEALSSELRQKSFKQMALLALEMTQKLI" }, "paralog": false, "identity": 0.9954545454545455, "score": 421.0, "evalue": 2.34e-152, "description": "Internal stop codon at 58686. Nonsense mutation around 58686.", "psc": { "uniref90_id": "UniRef90_P47295", "gene": "deoD", "product": "purine-nucleoside phosphorylase", "ec_ids": [ "2.4.2.1" ], "uniref50_id": "UniRef50_P47295", "cog_id": "COG0813", "cog_category": "F", "kegg_orthology_id": "K03784", "go_ids": [ "GO:0004731", "GO:0005829", "GO:0006139", "GO:0006152" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47295", "EC:2.4.2.1", "UniRef:UniRef50_P47295", "COG:COG0813", "COG:F", "KEGG:K03784", "GO:0004731", "GO:0005829", "GO:0006139", "GO:0006152" ] }, "pscc": { "uniref50_id": "UniRef50_P47295", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47295" ], "product": "Purine nucleoside phosphorylase DeoD-type" } }, "id": "JPCNHPFPBM_152", "locus": "GCA000292505_00112" }, { "type": "cds", "sequence": "contig_1", "start": 59079, "stop": 59750, "strand": "+", "frame": 3, "gene": "deoC", "product": "deoxyribose-phosphate aldolase", "db_xrefs": [ "COG:COG0274", "COG:F", "EC:4.1.2.4", "GO:0004139", "GO:0005737", "GO:0006018", "GO:0009264", "GO:0016052", "KEGG:K01619", "RefSeq:WP_014894546.1", "SO:0001217", "UniParc:UPI00027B32C0", "UniRef:UniRef100_UPI00027B32C0", "UniRef:UniRef50_P09924", "UniRef:UniRef90_P47296" ], "nt": "ATGAAATTAGAATACAACCGGATTATTGATAGCACCTTAGTCAAAGCTGATACGCTTCCCCATGAAATAGATACTTTATGTGCTGATGCTCATAAATACCAGTTTTTTGCAGTGTGTGTTAATCCTAGTTATGTTAGTTATGCTAAAAACATCTTGAAAAATACTGCAGTTCAACTCTGTTGTGTTGTTGGTTCCCCCTTAGGACAAACAACCCAAAAACAGAAGGTATATGAAGCTAAGATTGCTATTAAAGAGGGAGCGGATGAAATTGATATGGTAATGAATATTGCTGAGTTTAAAAAACGTTGTGCTTGTGTTATTACTGAAATTAGAGCTGTTAAAAAAGTGTGTGGCAAGCGTAAATTAAAAGTAATTATTGAAACTGCACTTTTAACAAATGATGAAATCAAAGATGCAGTTAATGTTTGCATTGATGGCAATGCAGATTATGTTAAAACTTCCACTGGTTTTTCTTTCCGTGGTGCATCTTTAGAAGATGTTCAGATTATGAATAATGCTGCAGCAAATTTAATTAAAATCAAAGCTTCAGGTGGGATTAAAACAGCAAAGCAATTTATAGATTTATTTCAAGCTGGAGCTAGTAGAATTGGAACTTCAAATGCGGTCCAAATAATGCAAGAATTAAACAAAATGAACCATGAATATCATTAA", "aa": "MKLEYNRIIDSTLVKADTLPHEIDTLCADAHKYQFFAVCVNPSYVSYAKNILKNTAVQLCCVVGSPLGQTTQKQKVYEAKIAIKEGADEIDMVMNIAEFKKRCACVITEIRAVKKVCGKRKLKVIIETALLTNDEIKDAVNVCIDGNADYVKTSTGFSFRGASLEDVQIMNNAAANLIKIKASGGIKTAKQFIDLFQAGASRIGTSNAVQIMQELNKMNHEYH", "aa_hexdigest": "f4a48de81fc684bf12cd8e7733848ee2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B32C0", "ncbi_nrp_id": "WP_014894546.1", "uniref100_id": "UniRef100_UPI00027B32C0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B32C0", "RefSeq:WP_014894546.1", "UniRef:UniRef100_UPI00027B32C0" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B32C0", "uniref90_id": "UniRef90_P47296" }, "psc": { "uniref90_id": "UniRef90_P47296", "gene": "deoC", "product": "deoxyribose-phosphate aldolase", "ec_ids": [ "4.1.2.4" ], "uniref50_id": "UniRef50_P09924", "cog_id": "COG0274", "cog_category": "F", "kegg_orthology_id": "K01619", "go_ids": [ "GO:0004139", "GO:0005737", "GO:0006018", "GO:0009264", "GO:0016052" ] }, "pscc": { "uniref50_id": "UniRef50_P09924", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P09924" ], "product": "Deoxyribose-phosphate aldolase" }, "genes": [ "deoC" ], "id": "JPCNHPFPBM_153", "locus": "GCA000292505_00113" }, { "type": "cds", "sequence": "contig_1", "start": 59737, "stop": 60660, "strand": "+", "frame": 1, "gene": null, "product": "Pyrimidine-nucleoside phosphorylase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A354QR12" ], "nt": "ATGAATATCATTAATTTAATTAATAAAAAACAACGCGGAAAAGCTTTAAATTTAGCTGAAATCAATTGGTTTGTTAATGCTGTTTTAAACAAAACCATTGCTGATTATCAAATTACTGCATTTTTGATGGCTATTTGGTTTAAAGGGATGAACCCAAATGAACTTTTTTTATTAACAAAAGCAATGGTGGATACTGGTGAAATTATTAAGTTTAATCACCATGGCAAGATTAGTGTTGATAAACATTCAACTGGTGGTATTGGTGATAAGGTTTCTTTAGCATTGGTTCCTATCTTAACTAGTTTAGGATTTAGTGTTGCTAAATTATCAGGAAGAGGCCTTGGTTATACTGGTGGAACAATTGATAAATTAGAAGCAGTTGGAGTTAAAACAGAATTAACTGACCAACAAGCACAAGCATGTTTAGATAAAAATGATTGTTTTATCATCGGACAAAGTAAGGACATCGCACCAGTTGATAAAGTACTTTATGGTTTAAGAGATATTACTGGAACAGTTGATAGTTTGCCTTTAATTGCATCTAGTATTATGTCTAAAAAGCTAGCAGTTATGAACGAGTATATTTTCATTGATCTTAAATATGGAAAAGGTGCCTTTTGTAAAACTAAGAAAATTGCTAACGAACTTGCAAAACTGATGCAAAGTATTGCTAAAAGTTTTAAAAGAAAGCTGTCTGTTAAATTAAGTGATATGAATCAAGTACTTGGTAAAGCTGTTGGCAATGTAATTGAAGTTAATGAAGCTGTTAACTTTCTAAAACAAGATTTAGATCAAGTAGGACAAGATTTTATTGATTTAATGCAAACAATTGTTATTAACATTCTACTTGAAACAAAACAAGCAAAAACCAAACAAAAGGCTATTGAACTTTATCAGGATGTTTTAACTAGTAAAAAAGCATGA", "aa": "MNIINLINKKQRGKALNLAEINWFVNAVLNKTIADYQITAFLMAIWFKGMNPNELFLLTKAMVDTGEIIKFNHHGKISVDKHSTGGIGDKVSLALVPILTSLGFSVAKLSGRGLGYTGGTIDKLEAVGVKTELTDQQAQACLDKNDCFIIGQSKDIAPVDKVLYGLRDITGTVDSLPLIASSIMSKKLAVMNEYIFIDLKYGKGAFCKTKKIANELAKLMQSIAKSFKRKLSVKLSDMNQVLGKAVGNVIEVNEAVNFLKQDLDQVGQDFIDLMQTIVINILLETKQAKTKQKAIELYQDVLTSKKA", "aa_hexdigest": "dbd041b3cc27638aecf487ace3562e61", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000A66C754", "query_cov": 0.8045602605863192, "subject_cov": 1.0, "identity": 0.514, "score": 236.0, "evalue": 5.88e-74, "valid": false, "product": "thymidine phosphorylase", "ec_ids": [ "2.4.2.4" ], "uniref50_id": "UniRef50_A0A354QR12", "go_ids": [ "GO:0006206" ] }, "pscc": { "uniref50_id": "UniRef50_A0A354QR12", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A354QR12" ], "product": "Pyrimidine-nucleoside phosphorylase" }, "genes": [], "id": "JPCNHPFPBM_154", "locus": "GCA000292505_00114" }, { "type": "cds", "sequence": "contig_1", "start": 60838, "stop": 61002, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07831.19" ], "nt": "ATGAAGAAAACAGATCAAATTGATCCAATGGCAGGGTTATTTTTAATGAAAAAAGATAATGAGTCTGTGGCAGTTGGAGACACTGTATTAAACCTGTATAGTTCTAGTCCTATTAGCAATGAAGATATCTCTGCTGCTCAAAAAACAATAATTATTAATAAATAA", "aa": "MKKTDQIDPMAGLFLMKKDNESVAVGDTVLNLYSSSPISNEDISAAQKTIIINK", "aa_hexdigest": "ef7fffa207b93e811b1ea6e6a1b2336b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07831.19", "name": "PYNP_C", "length": 53, "aa_cov": 0.9629629629629629, "hmm_cov": 1.0, "evalue": 5.194871264819346e-13, "score": 43.641700744628906, "start": 1, "stop": 52 } ], "seq_stats": { "molecular_weight": 5884.707, "isoelectric_point": 4.983893013000488 }, "id": "JPCNHPFPBM_155", "locus": "GCA000292505_00115" }, { "type": "cds", "sequence": "contig_1", "start": 61011, "stop": 61403, "strand": "+", "frame": 3, "gene": "cdd", "product": "cytidine deaminase", "db_xrefs": [ "COG:COG0295", "COG:F", "EC:3.5.4.5", "GO:0004126", "GO:0005737", "GO:0005829", "GO:0008270", "GO:0008655", "GO:0009972", "GO:0042802", "KEGG:K01489", "RefSeq:WP_010869308.1", "SO:0001217", "UniParc:UPI0000127407", "UniRef:UniRef100_P47298", "UniRef:UniRef50_P75051", "UniRef:UniRef90_P47298" ], "nt": "ATGAAGGTGAATTTAGAGTGGATAATTAAACAGTTACAAATGATAGTTAAAAGAGCATATACTCCCTTTTCTAACTTTAAAGTTGCATGTATGATTATTGCTAACAACCAAACTTTTTTTGGAGTTAACATTGAAAATTCTTCCTTTCCAGTAACTTTGTGTGCTGAAAGAAGCGCCATTGCTAGCATGGTTACAAGTGGTCATAGGAAAATTGATTATGTTTTTGTTTACTTCAATACTAAAAATAAGAGTAACTCACCCTGTGGAATGTGCAGACAAAACTTACTGGAATTTTCCCATCAAAAAACAAAGCTTTTTTGTATTGATAATGATAGTAGTTATAAACAATTTTCCATTGATGAATTATTAATGAATGGTTTTAAAAAGAGCTAA", "aa": "MKVNLEWIIKQLQMIVKRAYTPFSNFKVACMIIANNQTFFGVNIENSSFPVTLCAERSAIASMVTSGHRKIDYVFVYFNTKNKSNSPCGMCRQNLLEFSHQKTKLFCIDNDSSYKQFSIDELLMNGFKKS", "aa_hexdigest": "f7eb58a0e726f681ef33ce0e9201bbeb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000127407", "ncbi_nrp_id": "WP_010869308.1", "uniref100_id": "UniRef100_P47298", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000127407", "RefSeq:WP_010869308.1", "UniRef:UniRef100_P47298" ] }, "ips": { "uniref100_id": "UniRef100_P47298", "uniref90_id": "UniRef90_P47298" }, "psc": { "uniref90_id": "UniRef90_P47298", "gene": "cdd", "product": "cytidine deaminase", "ec_ids": [ "3.5.4.5" ], "uniref50_id": "UniRef50_P75051", "cog_id": "COG0295", "cog_category": "F", "kegg_orthology_id": "K01489", "go_ids": [ "GO:0004126", "GO:0005737", "GO:0005829", "GO:0008270", "GO:0008655", "GO:0009972", "GO:0042802" ] }, "pscc": { "uniref50_id": "UniRef50_P75051", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75051" ], "product": "Cytidine deaminase" }, "genes": [ "cdd" ], "id": "JPCNHPFPBM_156", "locus": "GCA000292505_00116" }, { "type": "cds", "sequence": "contig_1", "start": 61403, "stop": 62350, "strand": "+", "frame": 2, "gene": "manB", "product": "Phosphomannomutase", "db_xrefs": [ "COG:COG1109", "COG:G", "EC:5.4.2.8", "GO:0000287", "GO:0004615", "GO:0005975", "GO:0006166", "GO:0008973", "SO:0001217", "UniRef:UniRef50_P47299", "UniRef:UniRef90_P47299" ], "nt": "ATGGATAAACTTAGATTAGAAGTTGAAAGATGGTTAAATCATCCTAATGTTAATTGGGAGTTAAAACAACAAATTAAGGAGTTGAATGAATCAGAAATTCAAGAACTTTTTAGTTTGGAAAAACCTTTATTTGGCACTGCAGGTGTAAGAAACAAAATGGCACCAGGTTATCATGGTATGAATGTTTTTTCTTATGCCTATTTGACCCAAGGTTATGTTAAGTACATTGAATCAATCAATGAACCAAAGCTTCAACTACGGTTTTTAGTAGCACGTGATACAAGAAAAAATGGTGGTTTATTTTTAGAAACGGTTTGTGATGTAATTACATCTATGGGTCATTTGGCTTATGTGTTTGATGATAACCAGCCAGTTTCAACACCTCTAGTTTCCCATGTCATTTTTAAATATGGTTTTAGTGGAGGTATTAATATCACAGCTAGCCATAACCCTAAAGATGATAATGGTTTTAAGGTTTATGATCATACTGGTGCACAGCTTTTAGACACACAAACAAACCAATTGTTAAGTGATTTACCTTGTGTTACATCTATGCTAGATTTGGAATTACAACCAAATCCAAAGCTTGTCCATACTCTTGACAATGAAAAGGTTTATAAAAACTATTTCAGAGAGTTGAAAAAGGTGTTGGTTATTAACAACAACAATTTCAAAGACATTAAGGTAGTTTTTAGTGGGCTTAATGGGACTTCAGTTTGCTTAATGCAACGCTTTTTAAAGTACCTTGGTTATAGCAATATTATCAGTGTTGAGGAACAAAATTGGTTTGATGAGAATTTTGAAAATGCTCCTAACTTAAATCCAGAGTATAAAGATACATGGATATTAGCACAAAAATATGCTAAGAAAAATAATGCTAAGTTAATTATTATGGCAGACCCTGATGCTGATAGATTTGCAATTGCAGAGTTAAATAATAATCAATGA", "aa": "MDKLRLEVERWLNHPNVNWELKQQIKELNESEIQELFSLEKPLFGTAGVRNKMAPGYHGMNVFSYAYLTQGYVKYIESINEPKLQLRFLVARDTRKNGGLFLETVCDVITSMGHLAYVFDDNQPVSTPLVSHVIFKYGFSGGINITASHNPKDDNGFKVYDHTGAQLLDTQTNQLLSDLPCVTSMLDLELQPNPKLVHTLDNEKVYKNYFRELKKVLVINNNNFKDIKVVFSGLNGTSVCLMQRFLKYLGYSNIISVEEQNWFDENFENAPNLNPEYKDTWILAQKYAKKNNAKLIIMADPDADRFAIAELNNNQ", "aa_hexdigest": "3969a59ea9287cb35f6b67b0a45bb363", "start_type": "ATG", "rbs_motif": null, "genes": [ "manB" ], "truncated": "3-prime", "pseudogene": { "start": 61403, "stop": 62653, "observations": { "mutation": [ 62348 ], "stop": [ 62348 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47299", "query_cov": 1.0, "subject_cov": 0.5727272727272728, "identity": 0.9940000000000001, "score": 635.0, "evalue": 1.92e-226, "gene_start": 1, "gene_end": 315, "reference_start": 1, "reference_end": 315, "reference_sequence": "MDKLRLEVERWLNHPNVNWELKQQIKELNESEIQELFSLEKPLFGTAGVRNKMAPGYHGMNVFSYAYLTQGYVKYIESINEPKRQLRFLVARDTRKNGGLFLETVCDVITSMGHLAYVFDDNQPVSTPLVSHVIFKYGFSGGINITASHNPKDDNGFKVYDHTGAQLLDTQTNQLLSDLPCVTSMLDLELQPNPKFVHTLDNEKVYKNYFRELKKVLVINNNNFKDIKVVFSGLNGTSVCLMQRFLKYLGYSNIISVEEQNWFDENFENAPNLNPEYKDTWILAQKYAKKNNAKLIIMADPDADRFAIAELNNNQWHYFSGNETGAITAYYKLNHKVFKSPYIVSTFVSTYLVNKIAKRYGAFVHRTNVGFKYIGQAINELSQTNELVVGFEEAIGLITSDKLNREKDAYQAAALLLEIARHCKEQNITLLDFYKRILSEFGEYFNLTISHPFKATATDWKEEIKALFNQLINANLTEVAGFKVVKVHLDKQTNILEFGFENGWVKFRFSGTEPKLKFYFDLTNGTREALEKQAKKIYKFFVNLLKLNKA" }, "paralog": false, "identity": 0.9927884615384616, "score": 836.0, "evalue": 2.57e-308, "description": "Internal stop codon at 62348. Nonsense mutation around 62348.", "psc": { "uniref90_id": "UniRef90_P47299", "gene": "manB", "product": "Phosphomannomutase", "ec_ids": [ "5.4.2.8" ], "uniref50_id": "UniRef50_P47299", "cog_id": "COG1109", "cog_category": "G", "go_ids": [ "GO:0000287", "GO:0004615", "GO:0008973", "GO:0005975", "GO:0006166" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47299", "EC:5.4.2.8", "UniRef:UniRef50_P47299", "COG:COG1109", "COG:G", "GO:0000287", "GO:0004615", "GO:0008973", "GO:0005975", "GO:0006166" ] }, "pscc": { "uniref50_id": "UniRef50_P47299", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47299" ], "product": "Phosphomannomutase" } }, "id": "JPCNHPFPBM_157", "locus": "GCA000292505_00117" }, { "type": "cds", "sequence": "contig_1", "start": 62456, "stop": 62782, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02880.22" ], "nt": "TTGGTAAATAAGATTGCTAAAAGATATGGCGCTTTTGTGCATAGAACCAATGTTGGTTTTAAGTACATTGGTCAAGCAATTAATGAGTTATCACAAACAAACGAATTAGTTGTTGGTTTTGAAGAGGCAATTGGTTTAATAACTAGTGATAAATTAAACCGCGAGAAAGATGCTTATCAAGCTGCTGCATTATTGCTTGAGATTGCTAGACATTGCAAAGAACAAAACATCACGCTTTTAGATTTTTATAAAAGAATTCTTTCTGAGTTTGGTGAATATTTCAATTTAACAATATCTCATCCCTTTAAAGCTACTGCTACTGATTGA", "aa": "MVNKIAKRYGAFVHRTNVGFKYIGQAINELSQTNELVVGFEEAIGLITSDKLNREKDAYQAAALLLEIARHCKEQNITLLDFYKRILSEFGEYFNLTISHPFKATATD", "aa_hexdigest": "136ea89dfccaa978498ac34c51588023", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02880.22", "name": "PGM_PMM_III", "length": 83, "aa_cov": 0.7685185185185185, "hmm_cov": 0.9036144578313253, "evalue": 5.71095514357777e-14, "score": 47.37049865722656, "start": 1, "stop": 83 } ], "seq_stats": { "molecular_weight": 12285.895599999985, "isoelectric_point": 6.831103324890138 }, "id": "JPCNHPFPBM_158", "locus": "GCA000292505_00118" }, { "type": "cds", "sequence": "contig_1", "start": 62837, "stop": 63055, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00408.26" ], "nt": "GTGGCTGGTTTTAAAGTAGTTAAAGTCCATCTTGATAAACAAACAAATATCTTAGAGTTTGGTTTTGAAAATGGCTGGGTTAAATTCCGCTTTTCAGGTACTGAACCTAAATTGAAATTTTACTTTGACCTAACTAATGGCACTAGAGAGGCTCTAGAAAAGCAAGCTAAGAAAATTTATAAATTCTTTGTAAATTTACTCAAACTCAACAAAGCTTAA", "aa": "MAGFKVVKVHLDKQTNILEFGFENGWVKFRFSGTEPKLKFYFDLTNGTREALEKQAKKIYKFFVNLLKLNKA", "aa_hexdigest": "9704d7a0affff9369fc6f9fdfe87e693", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00408.26", "name": "PGM_PMM_IV", "length": 54, "aa_cov": 0.75, "hmm_cov": 0.9629629629629629, "evalue": 2.757181378105488e-08, "score": 28.900632858276367, "start": 7, "stop": 60 } ], "seq_stats": { "molecular_weight": 8461.833499999993, "isoelectric_point": 9.8733491897583 }, "id": "JPCNHPFPBM_159", "locus": "GCA000292505_00119" }, { "type": "cds", "sequence": "contig_1", "start": 63032, "stop": 63553, "strand": "-", "frame": 3, "gene": "nusG", "product": "transcription termination/antitermination protein NusG", "db_xrefs": [ "COG:COG0250", "COG:K", "GO:0005829", "GO:0006353", "GO:0006355", "GO:0031564", "GO:0032784", "GO:0140673", "SO:0001217", "UniRef:UniRef50_P47300", "UniRef:UniRef90_P47300" ], "nt": "ATGCTTAAAGGGATATCAATCAATCCTAGCAAACGAATTATGCTAACAAATACTGCCATTATTGAAATGGACAGTGATAAATTTGATGAAAAATTTCAATATATCTTAAAACAAAAACAAGCCATTCAAAAACCAAAAGAGGATGAAGATTCAGAAATTGTTGATGCTGAAAAACTAAAAGAAGCTTTTAAAAAACTACAAAATAGTCAAGAACAAGATGAATGGAAAGAAAAAGCAACGATTATTCAAAGTGAGCAAACCAAACTTGATCCATCAGTATTAGTTCCTTTTTTGGGCAAATATGAAATTCTTGATACTGACAATAAAGTTGAACAACTCTTTGAATTTAGTGTTGGTAATTTAGTAGAGGTACATTTAACTGATACTATTCATGTTCAAGGACAGATAAAAGCACTTTATCAAGGTACAGTTAACAAAGCAGTTGTAGAGATAGAATTAACATCTAAAACCCAATTAATTAACTTACCTTTAGAAAATTTAAGCTTTGTTGAGTTTGAGTAA", "aa": "MLKGISINPSKRIMLTNTAIIEMDSDKFDEKFQYILKQKQAIQKPKEDEDSEIVDAEKLKEAFKKLQNSQEQDEWKEKATIIQSEQTKLDPSVLVPFLGKYEILDTDNKVEQLFEFSVGNLVEVHLTDTIHVQGQIKALYQGTVNKAVVEIELTSKTQLINLPLENLSFVEFE", "aa_hexdigest": "405919f1e35823e1132b4e0609bf5aca", "start_type": "ATG", "rbs_motif": null, "genes": [ "nusG" ], "truncated": "3-prime", "pseudogene": { "start": 63032, "stop": 63853, "observations": { "mutation": [ 63661 ], "stop": [ 63661 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47300", "query_cov": 1.0, "subject_cov": 0.5474683544303798, "identity": 1.0, "score": 326.0, "evalue": 1.67e-110, "gene_start": 1, "gene_end": 173, "reference_start": 144, "reference_end": 316, "reference_sequence": "MQASELTPKWYVAPVSIKDEAVVKNLKAKIQALGFNHEIVDVKVLKEREVHEEVYSLKSGKLPRSLKNTTFNKWFVLDDYRYLRVKISEKNLLGRYIYIKMIYSEDAWRIVRNFPGITGIVGSSGRGALPIPLDEKDANNLEQMLKGISINPSKRIMLTNTAIIEMDSDKFDEKFQYILKQKQAIQKPKEDEDSEIVDAEKLKEAFKKLQNSQEQDEWKEKATIIQSEQTKLDPSVLVPFLGKYEILDTDNKVEQLFEFSVGNLVEVHLTDTIHVQGQIKALYQGTVNKAVVEIELTSKTQLINLPLENLSFVEFE" }, "paralog": false, "identity": 0.9963369963369964, "score": 531.0, "evalue": 6.31e-194, "description": "Internal stop codon at 63661. Nonsense mutation around 63661.", "psc": { "uniref90_id": "UniRef90_P47300", "gene": "nusG", "product": "transcription termination/antitermination protein NusG", "uniref50_id": "UniRef50_P47300", "cog_id": "COG0250", "cog_category": "K", "go_ids": [ "GO:0005829", "GO:0006353", "GO:0006355", "GO:0031564", "GO:0032784", "GO:0140673" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47300", "UniRef:UniRef50_P47300", "COG:COG0250", "COG:K", "GO:0005829", "GO:0006353", "GO:0006355", "GO:0031564", "GO:0032784", "GO:0140673" ] }, "pscc": { "uniref50_id": "UniRef50_P47300", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47300" ], "product": "Transcription termination/antitermination protein NusG" } }, "id": "JPCNHPFPBM_160", "locus": "GCA000292505_00120" }, { "type": "cds", "sequence": "contig_1", "start": 63659, "stop": 63982, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGCTAGTGAATTAACACCAAAGTGGTATGTAGCTCCTGTTAGTATTAAAGATGAAGCTGTTGTAAAAAATCTAAAAGCTAAAATTCAAGCTTTAGGATTTAATCATGAGATTGTTGATGTTAAAGTTCTAAAAGAAAGGGAAGTTCATGAAGAAGTTTATTCATTAAAATCAGGAAAACTTCCTCGTTCCTTAAAGAACACTACTTTTAACAAATGGTTTGTTCTTGATGATTACCGTTATCTTAGGGTAAAAATTAGTGAAAAAAATCTCCTTGGTAGATACATCTACATAAAGATGATTTATAGTGAAGATGCTTGA", "aa": "MQASELTPKWYVAPVSIKDEAVVKNLKAKIQALGFNHEIVDVKVLKEREVHEEVYSLKSGKLPRSLKNTTFNKWFVLDDYRYLRVKISEKNLLGRYIYIKMIYSEDA", "aa_hexdigest": "97401e671f986592ffc376eb3d641b5c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12579.512099999985, "isoelectric_point": 9.370753669738768 }, "id": "JPCNHPFPBM_161", "locus": "GCA000292505_00121" }, { "type": "cds", "sequence": "contig_1", "start": 64142, "stop": 64357, "strand": "-", "frame": 3, "gene": "secE", "product": "preprotein translocase subunit SecE", "db_xrefs": [ "COG:COG0690", "COG:U", "GO:0005886", "GO:0008320", "GO:0009306", "GO:0015450", "GO:0016020", "GO:0043952", "SO:0001217", "UniRef:UniRef50_P47301", "UniRef:UniRef90_P47301" ], "nt": "ATGGAAAAAAAACTGCCTTTTAGCTTTAAAAAGAAGGAAAAGCTAACTGCTTATGATGATGCTTCAATTCATGAGTTACATAAACAGCTCAAACTTAGAACAGAAGCCAAGAAAAGTAAAGATAAGGAAAGAACTAAAGAAAAAGAAAAGCATGAAAGTTTAGCAAAGGAAAAGAAACCCAAGCTTCCTTTTAAAAAACGAATTGTTAATTTATGA", "aa": "MEKKLPFSFKKKEKLTAYDDASIHELHKQLKLRTEAKKSKDKERTKEKEKHESLAKEKKPKLPFKKRIVNL", "aa_hexdigest": "798ee827a94f4f5c9d633acf03b073b7", "start_type": "ATG", "rbs_motif": null, "genes": [ "secE" ], "truncated": "5-prime", "pseudogene": { "start": 63986, "stop": 64357, "observations": { "mutation": [ 64108, 64144 ], "stop": [ 64108, 64144 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47301", "query_cov": 1.0, "subject_cov": 0.5772357723577236, "identity": 1.0, "score": 112.0, "evalue": 9.56e-31, "gene_start": 1, "gene_end": 71, "reference_start": 1, "reference_end": 71, "reference_sequence": "MEKKLPFSFKKKEKLTAYDDASIHELHKQLKLRTEAKKSKDKERTKEKEKHESLAKEKKPKLPFKKRIVNLWFGVDKEINKIVWVKGRQLIIIFLLILLVSGLMVGIFFGINQLLITLGIFKN" }, "paralog": false, "identity": 0.983739837398374, "score": 210.0, "evalue": 7.14e-72, "description": "Internal stop codon at 64108, 64144. Nonsense mutation around 64108, 64144.", "psc": { "uniref90_id": "UniRef90_P47301", "gene": "secE", "product": "preprotein translocase subunit SecE", "uniref50_id": "UniRef50_P47301", "cog_id": "COG0690", "cog_category": "U", "go_ids": [ "GO:0005886", "GO:0008320", "GO:0009306", "GO:0015450", "GO:0016020", "GO:0043952" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47301", "UniRef:UniRef50_P47301", "COG:COG0690", "COG:U", "GO:0005886", "GO:0008320", "GO:0009306", "GO:0015450", "GO:0016020", "GO:0043952" ] }, "pscc": { "uniref50_id": "UniRef50_P47301", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47301" ], "product": "Uncharacterized protein MG055" } }, "id": "JPCNHPFPBM_162", "locus": "GCA000292505_00122" }, { "type": "cds", "sequence": "contig_1", "start": 64747, "stop": 64908, "strand": "-", "frame": 1, "gene": null, "product": "MPN070 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75047", "UniRef:UniRef90_Q9ZB81" ], "nt": "ATGAAAGTATTTAAAAAACTCCATAACCTTAATGAGATTCTTTTGTTAATTAGTATCTTTTTTTTAGTTTGTATTATCTCTTTAGTAGGGATTGGTATCATCTTTGATTTAATTAGAAAAGCTAGTTTAAGTGCAATTAGAAGTGATCCTATTTTTTTTTAA", "aa": "MKVFKKLHNLNEILLLISIFFLVCIISLVGIGIIFDLIRKASLSAIRSDPIFF", "aa_hexdigest": "def4fbfd933bcbeb8f466afe4551334e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 64524, "stop": 64908, "observations": { "insertion": [ 64755 ], "stop": [ 64607, 64667 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9ZB81", "query_cov": 1.0, "subject_cov": 0.41732283464566927, "identity": 1.0, "score": 97.1, "evalue": 8.6e-25, "gene_start": 1, "gene_end": 53, "reference_start": 1, "reference_end": 53, "reference_sequence": "MKVFKKLHNLNEILLLISIFFLVCIISLVGIGIIFDLIRKASLSAIRSDPIFFNLRAVLIVLGVFAICFMIIQLVISIMIWKTINTACENIEPKFKKILHWSCFLPFGLLQLYCYQKIKLVKQADNL" }, "paralog": false, "identity": 0.984251968503937, "score": 235.0, "evalue": 8.36e-82, "description": "Internal stop codon at 64607, 64667. Frameshift due to insertion around 64755.", "psc": { "uniref90_id": "UniRef90_Q9ZB81", "product": "MPN070 family protein", "uniref50_id": "UniRef50_P75047", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9ZB81", "UniRef:UniRef50_P75047", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75047", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75047" ], "product": "Uncharacterized protein MG055.2 homolog" } }, "id": "JPCNHPFPBM_163", "locus": "GCA000292505_00123" }, { "type": "cds", "sequence": "contig_1", "start": 64895, "stop": 64996, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATATTGGTGTTAAGTTAAAAGATGCATGCAATTATTTAGCTAAAAAAATGCATTTAAAATCAAGTATGCTCTATACACTTTTCCATGAAAGTATTTAA", "aa": "MDIGVKLKDACNYLAKKMHLKSSMLYTLFHESI", "aa_hexdigest": "52022b2b22d050a7493e856737549861", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3829.575, "isoelectric_point": 8.93288440704346 }, "id": "JPCNHPFPBM_164", "locus": "GCA000292505_00124" }, { "type": "cds", "sequence": "contig_1", "start": 65129, "stop": 65728, "strand": "-", "frame": 3, "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "db_xrefs": [ "COG:COG0313", "COG:J", "EC:2.1.1.198", "GO:0000451", "GO:0005737", "GO:0008649", "GO:0008757", "GO:0070677", "KEGG:K07056", "SO:0001217", "UniRef:UniRef50_P47302", "UniRef:UniRef90_P47302" ], "nt": "ATGAAAACACTTAAAGTAGTTGCTACACCAATTGGTAATATTCAAGAGATAAGTGAAAGAGCTAAAAAAGCGTTACAAGATTGTGAAGTGTTATTCTGTGAAGATAGTAGAGTAACAAGAAAAATGCTGGATTTACTCAACATTGATTGCAAGCAAAAAAAGTTTGTTATTAACAACAGTTTTAAAGAAAAACAGAATTTAACTTTTGCTGAGGAATTTATAACTAACTTTAAGTGTTGTCTTGTTAGTGATGCAGGTTATCCTAGTTTATCTGATCCTGGTAATGAAATGATTAATTGGATCATATCAAAAAATAAGGAGATAAAAATCGAAGTTATTAATGGTCCTAGTGCATTAATGTGTGGATTGATCACTAGTGGTTTTAAAACAACACCACTTTTGTTTTTAGGTTTTTTAAGTCACAAACAAAATCAGCTCAAAAATTATTTAAGTACCTATCAAAATCAGAAAAGCACTATCGTTTTTTTTGAAGCAGTGCATAGGTTAGAAAATACTTTAGAAACTGTGAAAAATGTTTTTAAAAACAATGACGTTTTTATAGGAAGAGAATTAACTAAGTTACATGAATCACATTATTGA", "aa": "MKTLKVVATPIGNIQEISERAKKALQDCEVLFCEDSRVTRKMLDLLNIDCKQKKFVINNSFKEKQNLTFAEEFITNFKCCLVSDAGYPSLSDPGNEMINWIISKNKEIKIEVINGPSALMCGLITSGFKTTPLLFLGFLSHKQNQLKNYLSTYQNQKSTIVFFEAVHRLENTLETVKNVFKNNDVFIGRELTKLHESHY", "aa_hexdigest": "8d138a52482b83a29f059fc22b652a69", "start_type": "ATG", "rbs_motif": null, "genes": [ "rsmI" ], "truncated": "5-prime", "pseudogene": { "start": 64895, "stop": 65728, "observations": { "mutation": [ 65131 ], "stop": [ 65131 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47302", "query_cov": 1.0, "subject_cov": 0.7184115523465704, "identity": 0.995, "score": 399.0, "evalue": 1.06e-139, "gene_start": 1, "gene_end": 199, "reference_start": 1, "reference_end": 199, "reference_sequence": "MKTLKVVATPIGNIQEISERAKKALQDCEVLFCEDSRVTRKMLDLLNIDCKQKKFVINNSFKEKQNLTFAEEFITNFKCCLVSDAGYPSLSDPGNEMINWIISKNKEIRIEVINGPSALMCGLITSGFKTTPLLFLGFLSHKQNQLKNYLSTYQNQKSTIVFFEAVHRLENTLETVKNVFKNNDVFIGRELTKLHESHYWFNTSENTLPDITLKGEFVIVIDNQNINHQTLSSNQYLVYEIKKLMDIGVKLKDACNYLAKKMHLKSSMLYTLFHESI" }, "paralog": false, "identity": 0.9927797833935018, "score": 551.0, "evalue": 4.97e-202, "description": "Internal stop codon at 65131. Nonsense mutation around 65131.", "psc": { "uniref90_id": "UniRef90_P47302", "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "ec_ids": [ "2.1.1.198" ], "uniref50_id": "UniRef50_P47302", "cog_id": "COG0313", "cog_category": "J", "kegg_orthology_id": "K07056", "go_ids": [ "GO:0000451", "GO:0005737", "GO:0008649", "GO:0008757", "GO:0070677" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47302", "EC:2.1.1.198", "UniRef:UniRef50_P47302", "COG:COG0313", "COG:J", "KEGG:K07056", "GO:0000451", "GO:0005737", "GO:0008649", "GO:0008757", "GO:0070677" ] }, "pscc": { "uniref50_id": "UniRef50_P47302", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47302" ], "product": "Ribosomal RNA small subunit methyltransferase I" } }, "id": "JPCNHPFPBM_165", "locus": "GCA000292505_00125" }, { "type": "cds", "sequence": "contig_1", "start": 65863, "stop": 66246, "strand": "-", "frame": 1, "gene": "rnmV", "product": "ribonuclease M5", "db_xrefs": [ "COG:COG1658", "COG:J", "EC:3.1.26.8", "GO:0005737", "GO:0006364", "GO:0019843", "GO:0043822", "GO:0046872", "KEGG:K05985", "SO:0001217", "UniRef:UniRef50_P75045", "UniRef:UniRef90_P47303" ], "nt": "ATGGATCAAAAAGCACGTATAAAAATTGATGGGGTAATTGTTTGTGAGGGTAAAACTGACCAAGCTAAATTACAGCAAATTTTTGATGTAGATGTAATTACAACAAATGGTTCAGCTTTAAAAAAAGAAACTATTAACCTAATTAAAAAAATCAGTGAAAAGCAAACTGTTATCTTGTTATTAGATCCAGATCAACAAGGCAAAAAGATTAGAAGTAAATTAGAACAACATCTAACAAACTATTACAACTGTTATGTTGATATGAATGCAAGGTTAAAAAACGCTAAAAAAGCTGGTATTGCTGAAATGGAAACTAGTGCTTTAATTGCTGCTTTAAATAATCGTGTTGCAATCACAAAAAATGCTAATCAAAGCATATTATGA", "aa": "MDQKARIKIDGVIVCEGKTDQAKLQQIFDVDVITTNGSALKKETINLIKKISEKQTVILLLDPDQQGKKIRSKLEQHLTNYYNCYVDMNARLKNAKKAGIAEMETSALIAALNNRVAITKNANQSIL", "aa_hexdigest": "cae419be1a45db980494db4726f97918", "start_type": "ATG", "rbs_motif": null, "genes": [ "rnmV" ], "truncated": "5-prime", "pseudogene": { "start": 65710, "stop": 66246, "observations": { "mutation": [ 65727, 65865 ], "stop": [ 65727, 65865 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47303", "query_cov": 1.0, "subject_cov": 0.7134831460674157, "identity": 1.0, "score": 243.0, "evalue": 9.14e-81, "gene_start": 1, "gene_end": 127, "reference_start": 1, "reference_end": 127, "reference_sequence": "MDQKARIKIDGVIVCEGKTDQAKLQQIFDVDVITTNGSALKKETINLIKKISEKQTVILLLDPDQQGKKIRSKLEQHLTNYYNCYVDMNARLKNAKKAGIAEMETSALIAALNNRVAITKNANQSILWDQYLSLKLNDKKKRLLLCNNLNLPYFNHKQLFKKLNLLNLTFQDVWKHLK" }, "paralog": false, "identity": 0.9887640449438202, "score": 342.0, "evalue": 2.76e-122, "description": "Internal stop codon at 65727, 65865. Nonsense mutation around 65727, 65865.", "psc": { "uniref90_id": "UniRef90_P47303", "gene": "rnmV", "product": "ribonuclease M5", "ec_ids": [ "3.1.26.8" ], "uniref50_id": "UniRef50_P75045", "cog_id": "COG1658", "cog_category": "J", "kegg_orthology_id": "K05985", "go_ids": [ "GO:0005737", "GO:0006364", "GO:0019843", "GO:0043822", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47303", "EC:3.1.26.8", "UniRef:UniRef50_P75045", "COG:COG1658", "COG:J", "KEGG:K05985", "GO:0005737", "GO:0006364", "GO:0019843", "GO:0043822", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P75045", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75045" ], "product": "Ribonuclease M5" } }, "id": "JPCNHPFPBM_166", "locus": "GCA000292505_00126" }, { "type": "cds", "sequence": "contig_1", "start": 66225, "stop": 67316, "strand": "-", "frame": 2, "gene": "prs", "product": "Ribose-phosphate pyrophosphokinase", "db_xrefs": [ "COG:COG0462", "COG:F", "EC:2.7.6.1", "GO:0000287", "GO:0002189", "GO:0004749", "GO:0005524", "GO:0005737", "GO:0006015", "GO:0006164", "GO:0009156", "GO:0016301", "KEGG:K00948", "SO:0001217", "UniRef:UniRef50_P47304", "UniRef:UniRef90_P47304" ], "nt": "ATGGCAAAATTAAAGTGGAAATTTGATTGGCAAAACCTAAAAAGAAATTTGACAAACGTGAAACTATTAAAAAGAAAACAATCCGACGCGAGCTTGAAGCTGAGTATCGATAATAAAAAGCACGTTATTTTTAGTTTATCTAAAAGCAAAACACTGGTTGAAAATATCTGCAAAAAACTAAATATTTCAGAGGGCAAAATGGTTTGCGAACACTTTGCTGATGGTGAAACTTATATCCGTTTTGATGAATCAGTTCGTAACAAAGATATCTATATTTTTCAATCAACCTGTCCTAATGTTAACGATAGCTTAATGGAACTTTTAATTGCTATTGATGCATTGAAAAGAGGTAGTGCTAAAAGTATTACTGCCATTCTACCCTATTATGGATATGCAAGACAAGATAGAAAAACAAAAGGAAGAGAACCAATTACCAGTAAATTGATTGCTGATATGTTAACAAAAGCAGGTGCTAACAGGGTTGTTCTAACTGACATTCATAGTGATCAAACCCAAGGTTTTTTTGATATTCCCGTTGATTCTTTAAGAACTTATCACATCTTTCTTTTTAGAGTTATAGAACTACTTGGTAAAAAAGACTTGGTGGTTGTTTCCCCTGATTATGGTGGGGTTAAAAGAGCAAGGTTAATTGCAAATACACTAGAACTACCATTAGCCATTATTGATAAAAGAAGACCATCTCATAATGTTGCTGAATCAATTAATGTTTTAGGTGAAGTGAAAAATAAAAACTGTTTAATAGTTGATGACATGATAGATACTGGTGGTACAGTAATTGCAGCAGCCAAGCTATTACAAAAAGAACAAGCTAAAAAAGTGTGTGTAATGGCAACTCATGGTTTGTTTAACAATGATGCAGAACAAAAGTTTATGGAAGCATTTGATCAAAAACTAATTGATTTCTTGTTTGTATCAAACTCTATTCCTCAATATAAGTTTAAAGCTGTAAAGCAGTTTGAAGTAGTTGATCTAGCATCTTTATATGAAGAGGTTGTTCTGTGTTACGCTAACAGCTTATCAGTTTCTGCAATATATGAACGTCACATTGAATGGATCAAAAAGCACGTATAA", "aa": "MAKLKWKFDWQNLKRNLTNVKLLKRKQSDASLKLSIDNKKHVIFSLSKSKTLVENICKKLNISEGKMVCEHFADGETYIRFDESVRNKDIYIFQSTCPNVNDSLMELLIAIDALKRGSAKSITAILPYYGYARQDRKTKGREPITSKLIADMLTKAGANRVVLTDIHSDQTQGFFDIPVDSLRTYHIFLFRVIELLGKKDLVVVSPDYGGVKRARLIANTLELPLAIIDKRRPSHNVAESINVLGEVKNKNCLIVDDMIDTGGTVIAAAKLLQKEQAKKVCVMATHGLFNNDAEQKFMEAFDQKLIDFLFVSNSIPQYKFKAVKQFEVVDLASLYEEVVLCYANSLSVSAIYERHIEWIKKHV", "aa_hexdigest": "dd7d383aef5d31152815e2ebc3691c66", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47304", "query_cov": 0.9146005509641874, "subject_cov": 1.0, "identity": 0.997, "score": 650.0, "evalue": 5.34e-235, "valid": true, "gene": "prs", "product": "Ribose-phosphate pyrophosphokinase", "ec_ids": [ "2.7.6.1" ], "uniref50_id": "UniRef50_P47304", "cog_id": "COG0462", "cog_category": "F", "kegg_orthology_id": "K00948", "go_ids": [ "GO:0005737", "GO:0002189", "GO:0005524", "GO:0016301", "GO:0000287", "GO:0004749", "GO:0006015", "GO:0006164", "GO:0009156" ] }, "pscc": { "uniref50_id": "UniRef50_P47304", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47304" ], "product": "Ribose-phosphate pyrophosphokinase" }, "genes": [ "prs" ], "id": "JPCNHPFPBM_167", "locus": "GCA000292505_00127" }, { "type": "cds", "sequence": "contig_1", "start": 67291, "stop": 67641, "strand": "-", "frame": 1, "gene": "smpB", "product": "SsrA-binding protein", "db_xrefs": [ "COG:COG0691", "COG:O", "GO:0003723", "GO:0005829", "GO:0006450", "GO:0070929", "KEGG:K03664", "SO:0001217", "UniRef:UniRef50_P75043", "UniRef:UniRef90_P47305" ], "nt": "ATGTTAATTCTTGTTAACAATCCTAAGGCTAAATATGACTATCATTTAATGGAATCTTATTGTGCTGGAATAGTTTTAAAAGGAAGTGAAGTTAAAGCTTTAAGTTTAGGTCAAGGTAGTTTAAAAGAAGCTTATGTTTTTGTTAAAAACAATGAGCTTTTTTTAGAACAGTTCACTATTCCACCTTATAGTTTTGCAGGTCCATTAAATCACGCTTCAGATAGAATTAAGAAACTTTTATTAAATAAACATGAAATTAAACAAATTATCAATAAAAAACAACAACAATCTTTATCTGTAATCCCAAGTAAAGTATTCTTTAGAAATGGCAAAATTAAAGTGGAAATTTGA", "aa": "MLILVNNPKAKYDYHLMESYCAGIVLKGSEVKALSLGQGSLKEAYVFVKNNELFLEQFTIPPYSFAGPLNHASDRIKKLLLNKHEIKQIINKKQQQSLSVIPSKVFFRNGKIKVEI", "aa_hexdigest": "1c2fff54eab8bedf7143b6f1d9747546", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47305", "query_cov": 1.0, "subject_cov": 0.8, "identity": 1.0, "score": 228.0, "evalue": 2.41e-75, "valid": true, "gene": "smpB", "product": "SsrA-binding protein", "uniref50_id": "UniRef50_P75043", "cog_id": "COG0691", "cog_category": "O", "kegg_orthology_id": "K03664", "go_ids": [ "GO:0003723", "GO:0005829", "GO:0006450", "GO:0070929" ] }, "pscc": { "uniref50_id": "UniRef50_P75043", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75043" ], "product": "SsrA-binding protein" }, "genes": [ "smpB" ], "id": "JPCNHPFPBM_168", "locus": "GCA000292505_00128" }, { "type": "cds", "sequence": "contig_1", "start": 67648, "stop": 67914, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00535.33" ], "nt": "ATGAAATTATCTGTAATTATACCTACTTACAATTGTGCATCATTTATTGAAAAAACAATTAATTCAATTGTTAAAAATAGACCTAATGATTTGGAAATAGAAGTTTTAATTATTGATGATGGATCAATTGACAATACTAACAAAGTTATTAAGAAAATTCAAGACCAAATTAATAATTTAACTTTGCAGTATTTTTACAAAAGTAATGGTAACTGGGGTAGTGTTATTAATTATGTTAGAAACAATAAACTAGCAAAAGGGGAATGA", "aa": "MKLSVIIPTYNCASFIEKTINSIVKNRPNDLEIEVLIIDDGSIDNTNKVIKKIQDQINNLTLQYFYKSNGNWGSVINYVRNNKLAKGE", "aa_hexdigest": "92e757a44630f3b5f0e61d9cbfd88183", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00535.33", "name": "Glycos_transf_2", "length": 84, "aa_cov": 0.9545454545454546, "hmm_cov": 0.9404761904761905, "evalue": 1.4008970579059538e-17, "score": 59.23566436767578, "start": 4, "stop": 87 } ], "seq_stats": { "molecular_weight": 10047.392199999997, "isoelectric_point": 8.732774543762208 }, "id": "JPCNHPFPBM_169", "locus": "GCA000292505_00129" }, { "type": "cds", "sequence": "contig_1", "start": 67934, "stop": 68092, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATTTTTTCAAAAAAAACAATTTCTATTTTTCAAAAATATGCCCAAAAACAAAGATATGATGCGATTATTTTTGACTACTATAAATGCTGAAAAAAGTTTTTGTGAAAAATTCCTACCTATGCAAGGTTTAGAAAAGAAATTAAAGGTGAATTGA", "aa": "MTFFQKKQFLFFKNMPKNKDMMRLFLTTINAEKSFCEKFLPMQGLEKKLKVN", "aa_hexdigest": "941594f13135f7075475b6bd2801b7ba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6339.647499999999, "isoelectric_point": 10.01376132965088 }, "id": "JPCNHPFPBM_170", "locus": "GCA000292505_00130" }, { "type": "cds", "sequence": "contig_1", "start": 68089, "stop": 68301, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAAACAAACACCTTTTTGTATTCCCTTAGCTAAGTTTTTTAAAAATGAGGTTTTCTATCAACTTCCTAAACTAAGAGAAAATGTTGGTTTTCAAGACGCTATTTATACGATGCATGCATTACAAATTGCAAATAATGTTTTCCATGTTTCTAAAGCTGGAGGATATTACTTTTTTAAAAGGGTAGGTAACTCTATGAGTATCCCTTGA", "aa": "MKKQTPFCIPLAKFFKNEVFYQLPKLRENVGFQDAIYTMHALQIANNVFHVSKAGGYYFFKRVGNSMSIP", "aa_hexdigest": "21a3cece4e3be010d522798d65e95ecf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8121.4417, "isoelectric_point": 9.779418754577634 }, "id": "JPCNHPFPBM_171", "locus": "GCA000292505_00131" }, { "type": "cds", "sequence": "contig_1", "start": 68589, "stop": 69035, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCACTTCTTATTAACAGCTAACATAGTGGTATTGCTTATAATCATCATGTTTGCAGCAACACTTGGTATTGGTAGTGCAGCTGGGTTTGCTTTAATTAGTATCTTTTCATTTATCGGTGGTGCATTTGCTTGGAGTTTATCAAGTTCAAATTTAATCCTGCCATATGAATTTAAAGATTACAAGAAGAATGAACTTCCTATTCTTTTTGGATTTTGTTGGGGCTTTGGTTATATTGCTTACACCTTATTTGACATTACCCAATCAGTATTTCTCCAAGCGCCTGTTTTAATTCAAGGAAAAGGTACAAGTATTTTACCAGGGGTAATTGTAAGTATTGTTTTCTTCTTAGGACTTATTGCACTAGCAAACCTAATTGTTAAGTTCTTTCCTGCTTGTTGGATTAAAGATGGTGATCAATTGGTTCAGGAAATGACAAGAAAATGA", "aa": "MHFLLTANIVVLLIIIMFAATLGIGSAAGFALISIFSFIGGAFAWSLSSSNLILPYEFKDYKKNELPILFGFCWGFGYIAYTLFDITQSVFLQAPVLIQGKGTSILPGVIVSIVFFLGLIALANLIVKFFPACWIKDGDQLVQEMTRK", "aa_hexdigest": "f78a76e8d077db6d7b3fa4e0b2926108", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16253.248899999984, "isoelectric_point": 7.752804374694825 }, "id": "JPCNHPFPBM_172", "locus": "GCA000292505_00132" }, { "type": "cds", "sequence": "contig_1", "start": 69267, "stop": 69977, "strand": "-", "frame": 2, "gene": null, "product": "Major facilitator superfamily transporter MG061", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47307", "UniRef:UniRef90_P47307" ], "nt": "TTGAGAGCAGTAGGTTCTATTCTTTGTGGAGTTGTTGTTTTGAAATTTGGTTATCGTTATGCGGTTTTAATCATGATGGGAATCATGTGTGTTTGTTTTCCCTTTCTTATTATAGGAGACCCTTTGAATGGTCATAATCAATTAACTTTATTAAGACCTTTATCAGATTCAGTAAAAACTCAATTAAGTTCACTAAGTAGCAATTTACAAGTGGGTCAATTACTTGGACCGGTGATGATCAATGGTAAAACGATGCTTGCAGATGGAACAAGTGTTGAACTTATTAAGGGTTTGGATGGTAATAGTATTGGTACAGCTGCATCAATTACTGGTTATGCATTGTTTATTATCTTTAGAAGCACAATTGCTATTGGTGGAACTACCTTAGTAGTTTATACTCAACCAGCAATTGCTAATCTTTCTTCAAACAGAAAAAAATCAATCCTATCTAATGCTAACCTTTGGGGTTTTAACATTGGGATTGCAGTAGTTTTCACACCATTTTTATTTGAACAAGTTCAACAAGTAGCATCTCAATACTGGGTTTACATTATGACTGTGATGATTTTGGTTGTGTTTGCTAACTTATGTTTATTTTTATGGTTTGAAAGCAAGATAGATCACATCTTTCCTCAAAAGCAAACTAAGGAAAATATGAGTTTAACTACTCAACCAAAATCCATAGATATCTTAAAGAATAAAACAACATGA", "aa": "MRAVGSILCGVVVLKFGYRYAVLIMMGIMCVCFPFLIIGDPLNGHNQLTLLRPLSDSVKTQLSSLSSNLQVGQLLGPVMINGKTMLADGTSVELIKGLDGNSIGTAASITGYALFIIFRSTIAIGGTTLVVYTQPAIANLSSNRKKSILSNANLWGFNIGIAVVFTPFLFEQVQQVASQYWVYIMTVMILVVFANLCLFLWFESKIDHIFPQKQTKENMSLTTQPKSIDILKNKTT", "aa_hexdigest": "5040f8586b191b71c17a1f3bf0093ea0", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 68964, "stop": 70223, "observations": { "mutation": [ 69038, 69110, 69206, 69269, 69992 ], "stop": [ 69038, 69110, 69206, 69269, 69992 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47307", "query_cov": 1.0, "subject_cov": 0.41622574955908287, "identity": 0.996, "score": 451.0, "evalue": 5.4e-155, "gene_start": 1, "gene_end": 236, "reference_start": 83, "reference_end": 318, "reference_sequence": "MENKSQKKVSDLKLITLWIIVIFGYLLFVVEWFVIDRISGKPTGILTQSTTTLPQYSGWLSSFFTENAGQIATSSTNWTITLLRAVGSILCGVVVLKFGYRYAVLIMMGIMCVCFPFLIIGDPLNGHNQLTLLRPLSDSVKTQLSSLSSNLQVGQLLGPVMINGKTMLADGTSVELIKGLDGNSIGTAASITGYALFIIFRSTIAIGGTTLVVYTQPAIANLSSNRKKSILSNANLWGFNIGIAVVFTPFLFEQVQQVASQYWVYIMTVMILVVFANLCLFLWFESKIDHIFPQKQTKENMSLTTQPKSIDILKNKTTWKLIGVYGIVLILIVNPLTPAWFSILQTVSPSGSNGLISTGVYTTGLATLAIFWVIGYALGFVVFSPFNKTIYDKKRWMHFLLTANIVVLLIIIMFAATLGIGSAAGFALISIFSFIGGAFAWSLSSSNLILPYEFKDYKKNELPILFGFCWGFGYIAYTLFDITQSVFLQAPVLIQGKGTSILPGVIVSIVFFLGLIALANLIVKFFPACWIKDGDQLVQEMTRKWKLNEWQFVIANKQKNRYSELLK" }, "paralog": false, "identity": 0.9856801909307876, "score": 796.0, "evalue": 1.42e-293, "description": "Internal stop codon at 69038, 69110, 69206, 69269, 69992. Nonsense mutation around 69038, 69110, 69206, 69269, 69992.", "psc": { "uniref90_id": "UniRef90_P47307", "product": "Major facilitator superfamily transporter MG061", "uniref50_id": "UniRef50_P47307", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47307", "UniRef:UniRef50_P47307", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47307", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47307" ], "product": "Major facilitator superfamily transporter MG061" } }, "id": "JPCNHPFPBM_173", "locus": "GCA000292505_00133" }, { "type": "cds", "sequence": "contig_1", "start": 69990, "stop": 70223, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAAATAAAAGTCAAAAAAAAGTAAGTGATCTGAAACTTATTACCCTGTGGATCATAGTAATTTTTGGGTATCTATTGTTTGTAGTTGAATGGTTTGTAATTGATCGAATTAGTGGTAAACCAACTGGTATACTTACTCAATCAACCACCACTTTACCACAGTACAGTGGTTGGTTAAGTTCTTTCTTTACTGAAAATGCTGGGCAAATAGCCACATCTTCCACCAACTGA", "aa": "MENKSQKKVSDLKLITLWIIVIFGYLLFVVEWFVIDRISGKPTGILTQSTTTLPQYSGWLSSFFTENAGQIATSSTN", "aa_hexdigest": "9f8e71f0f49ac5cd20d65e72a80bf55c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8660.895099999994, "isoelectric_point": 8.06423740386963 }, "id": "JPCNHPFPBM_174", "locus": "GCA000292505_00134" }, { "type": "tRNA", "sequence": "contig_1", "start": 70391, "stop": 70479, "strand": "-", "gene": "trnS", "product": "tRNA-Ser(gct)", "amino_acid": "Ser", "anti_codon": "gct", "score": 76.9, "nt": "GGGAGCGTACTCAAGTGGTTAAGAGGACACCCTGCTAAGGTGTTAGATCGTTCTACGGTGCGTGGGTTCGAATCCCACCGCTTCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 70444, 70446 ], "id": "JPCNHPFPBM_34", "locus": "GCA000292505_00135" }, { "type": "cds", "sequence": "contig_1", "start": 70528, "stop": 70794, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00359.27" ], "nt": "ATGTTTAAAAATTTATTAAGACCATCCCTATTTTTTAACTGGAGCCAAAAAACATTTAAAAATAAATTTTCGTTTTTAAAACAAGCAGCAAATGCATTACAAAAACAGGCTGTTATCAATGATAACAATGTTGCATTTGAAGCTTTAAAAAAGCGTGAAGAAGAGATTACAACTGGAATTATTACTAGTTTAGCTTTACCTCACTTACAAAGTCAAAGTGTTATAGAACCTTTTGTTGCTGTATTTAAGGTTAAAAACTTAGATTGA", "aa": "MFKNLLRPSLFFNWSQKTFKNKFSFLKQAANALQKQAVINDNNVAFEALKKREEEITTGIITSLALPHLQSQSVIEPFVAVFKVKNLD", "aa_hexdigest": "69befee4b3c9dac3ce986b5e98e60c3f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00359.27", "name": "PTS_EIIA_2", "length": 71, "aa_cov": 0.8068181818181818, "hmm_cov": 1.0, "evalue": 1.2917106571351258e-14, "score": 49.511775970458984, "start": 16, "stop": 86 } ], "seq_stats": { "molecular_weight": 10084.609399999996, "isoelectric_point": 9.821838951110838 }, "id": "JPCNHPFPBM_175", "locus": "GCA000292505_00136" }, { "type": "cds", "sequence": "contig_1", "start": 70822, "stop": 72570, "strand": "+", "frame": 1, "gene": "fruA", "product": "PTS system fructose-specific EIIABC component", "db_xrefs": [ "COG:COG1762", "COG:GT", "GO:0005351", "GO:0005886", "GO:0009401", "GO:0016301", "GO:0022877", "GO:0090563", "SO:0001217", "UniRef:UniRef50_P47308", "UniRef:UniRef90_P47308" ], "nt": "TTGATATTTTTAATTGGTGTTCCTGTTGACAAAACCAATTTGCATCTTGATTTCATTAGTAACTTTTCCAAGTTAATGTTGAATGAAACATTTGCAAGTAAGGTTTTAAATGTCACTAGCTATAACGGCTTGATTAAACTAATTGATCTTTTTAACCAACAAAAAGTGCAAGACCAACCAGCTGTTGAAACAAAAAAAGAGTATGACTTTGTTGCTGTAACAGCATGCCCAACTGGCATTGCCCATACTTTCATGGCTAAAGAAGCGTTGGAAGCATTTGCAAAAAAGCATAACTTATATGTAAAAGTTGAAACTCAAGGTACAGATGGGATACAAAATCAACTTACAAGTGACGATATTAATAACGCTAAAGGTGTTATTCTTGCTTGTGATCGTTTAATTGATTTCTCTAGGTTTTATGCAAATAAGAATGTGATTGAAGTGTCAACTACTAAAGCCATTAAAAAACCTGATGAGGTGTATGAATTAATTAAAAACCAAAAGGGTAAACAGCTTGCAAATAGTGCTAAACCAACTAATCAAACCCAATTAGCTGAAAGTGAAGGGGAATTCAACTTTAATAATTTTCACAAGCGGATTTATCGTGCCATTTTAAGTGGTGTTTCTTACATGCTACCATTCGTTGTTTTTGGTGGGATATTAATTGCACTCTCTTTTCTAATTGATATAAATAACGCTAATAATGCTGGTGGTAACTTTGGCACAATTAATCCTGTTGCTAACTGGTTAAATAAGCTAGGAGGAATTTCCTTTAGTTTAATTGTTCCTATCTTATCTGCATACATTGCATATGCATTGGTATCAAGACAGGGATTATTACCTGGTTTTGTTGTTGGTTTAATCTCTTCAGGACAATTTTTATTAAATATTGTTCTGACCAATGGAACAATTGAATGGTTAGCACCATCACAAGTATCAAGTGGCTTTTTTGGTGCTATCTTTGGAGGACTTTTGAGTGCTTGCTTAATAATTGTTCAACAAAACTACATCTATAAAAAATTACCTCAATCTTTGCAGGGAATTAAAAACATCTTGTTTATTCCCTTGTTTGGTACTTTATTCACTGCTGGTTTATTTTGGGTTATTAACATTCCTTTAATTTATCTAAACTATGGATTAAGTCTGTTTTTAAATATTATGAACAGCCCTATCCTAGCACCTTTACTTGGTTTTGTAATTGGGTTGATGATGTGCTTTGATTTAGGGGGGCCAATTAATAAAGCAGCTTATGTTTTTGGTGTTGTTTCTTTACAAAATCAAAATGCAGGAACAATTGCGATGGCTGCAGCTATGCTATCAGGGATGGTACCTCCTTTATCAATTGCTTTGGCAGCTTCCATTCGAAAGAGCTGCTTTGATAAACAGGAATTACCTGCAGCTTATGCTTGTTATCTGATGGGATTGAGTTTTATTAGTGAAGGTGCTATCCCATTTGTTGTTAAAAAACCTAAGGTGATGTTAACTGCTAACTTAATTGCTGGAGCAATTTGTGGAGCATTAACAGGAGCATTTGCCTTATCAATTCGTGCTCCTCATGGCGGTGTTTTTGTGTTTGCACTTTTAAAAACTACTTTACAAGGGATTGAAGGAGCTACATTACAAACTGGAGTTGGCATTGGTTTGGCATTGGTTTGTTTAATAATTAGTATGATAGTTGGTAGTAGTATTATCATTGGCTATGACTTGATTGCAAAACATAACCAAAGAAAGCAAAATCTGAATAGTTAA", "aa": "MIFLIGVPVDKTNLHLDFISNFSKLMLNETFASKVLNVTSYNGLIKLIDLFNQQKVQDQPAVETKKEYDFVAVTACPTGIAHTFMAKEALEAFAKKHNLYVKVETQGTDGIQNQLTSDDINNAKGVILACDRLIDFSRFYANKNVIEVSTTKAIKKPDEVYELIKNQKGKQLANSAKPTNQTQLAESEGEFNFNNFHKRIYRAILSGVSYMLPFVVFGGILIALSFLIDINNANNAGGNFGTINPVANWLNKLGGISFSLIVPILSAYIAYALVSRQGLLPGFVVGLISSGQFLLNIVLTNGTIEWLAPSQVSSGFFGAIFGGLLSACLIIVQQNYIYKKLPQSLQGIKNILFIPLFGTLFTAGLFWVINIPLIYLNYGLSLFLNIMNSPILAPLLGFVIGLMMCFDLGGPINKAAYVFGVVSLQNQNAGTIAMAAAMLSGMVPPLSIALAASIRKSCFDKQELPAAYACYLMGLSFISEGAIPFVVKKPKVMLTANLIAGAICGALTGAFALSIRAPHGGVFVFALLKTTLQGIEGATLQTGVGIGLALVCLIISMIVGSSIIIGYDLIAKHNQRKQNLNS", "aa_hexdigest": "f27752d481a3576df6d8cf961fb44730", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47308", "query_cov": 1.0, "subject_cov": 0.8558823529411764, "identity": 0.995, "score": 1105.0, "evalue": 0.0, "valid": true, "gene": "fruA", "product": "PTS system fructose-specific EIIABC component", "uniref50_id": "UniRef50_P47308", "cog_id": "COG1762", "cog_category": "GT", "go_ids": [ "GO:0005886", "GO:0005351", "GO:0016301", "GO:0022877", "GO:0090563", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P47308", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47308" ], "product": "PTS system fructose-specific EIIABC component" }, "genes": [ "fruA" ], "id": "JPCNHPFPBM_176", "locus": "GCA000292505_00137" }, { "type": "cds", "sequence": "contig_1", "start": 72665, "stop": 73432, "strand": "+", "frame": 2, "gene": "fruK", "product": "Putative 1-phosphofructokinase", "db_xrefs": [ "COG:COG1105", "COG:G", "EC:2.7.1.56", "GO:0005524", "GO:0005829", "GO:0008443", "GO:0008662", "SO:0001217", "UniRef:UniRef50_Q49396", "UniRef:UniRef90_Q49396" ], "nt": "ATGAAATCACTTGGTTTTGATCCAACTGTCATTACTTTTTTGGGACAACCCACTAAAAACTTATTTTTAGAGTTGGTAAAACCTTATGATCTAAATATAGTTAGCTTCATTTCTGAAACTAAAACAAGAATTAACCTTAAGTTATTAAAAGATGAAAAAACTACTGAAATTAATGATTTAAGTCCTTTAATAACAGATGCTAATCTAACTGAATTGTTAACTTTTTTAAAAGCTAATGTTAAGAATAATGATTTGGTTATCATCAACGGAAGATTTAAATTTGAAGCTTTAGAAAAAGTTCTAAACTTGGTCTTTACATTAACAGAAAATGTGGTTATAGATGTTGATGAAAGCAAAATGTTAACGCTTTTAAATCAGTCTAAACCACTAGTTATGAAACCTAACATTGATGAGTTTCAAACTATGATTAATACTTTTTTTCACGATCAACAAAGCTTAATAGCAGCAATTAAAAAATTTCATTACTGTAAGCTCTTATTATTATCTGATGGTGACAAAGGAGCTTATCTTTTTGATCAGAATAAGTTATTGTTTGTAAGTTCTATCACTCCTAAACAAGTAGTTAGCACCACAGGAGCAGGTGATACTTTGTTGGCAGTTTTTTTAGCAAATTTAATTCTAAAGGTAGATTTAAAAACTGCTTTGATTAAAGCAACTAACTATGCAAGTGCAACAATTAGTAAGTTAGGTGTTGTTGATAGTAAAGACAAAATTAGTGTTATAACCCCAAAAAGTTACTATTTATAA", "aa": "MKSLGFDPTVITFLGQPTKNLFLELVKPYDLNIVSFISETKTRINLKLLKDEKTTEINDLSPLITDANLTELLTFLKANVKNNDLVIINGRFKFEALEKVLNLVFTLTENVVIDVDESKMLTLLNQSKPLVMKPNIDEFQTMINTFFHDQQSLIAAIKKFHYCKLLLLSDGDKGAYLFDQNKLLFVSSITPKQVVSTTGAGDTLLAVFLANLILKVDLKTALIKATNYASATISKLGVVDSKDKISVITPKSYYL", "aa_hexdigest": "0d21cfdaeeac6761160d13a7af20f43d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q49396", "query_cov": 1.0, "subject_cov": 0.8415841584158416, "identity": 1.0, "score": 486.0, "evalue": 9.74e-173, "valid": true, "gene": "fruK", "product": "Putative 1-phosphofructokinase", "ec_ids": [ "2.7.1.56" ], "uniref50_id": "UniRef50_Q49396", "cog_id": "COG1105", "cog_category": "G", "go_ids": [ "GO:0005829", "GO:0008662", "GO:0005524", "GO:0008443" ] }, "pscc": { "uniref50_id": "UniRef50_Q49396", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49396" ], "product": "Putative 1-phosphofructokinase" }, "genes": [ "fruK" ], "id": "JPCNHPFPBM_177", "locus": "GCA000292505_00138" }, { "type": "cds", "sequence": "contig_1", "start": 73443, "stop": 73646, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_014894126.1", "SO:0001217", "UniParc:UPI00027B32C6", "UniRef:UniRef100_UPI00027B32C6", "UniRef:UniRef50_UPI00027B32C6", "UniRef:UniRef90_UPI00027B32C6" ], "nt": "ATGGTAAAAAGAAAGCGAAAGCCTAAGCTTAATTCGCGTAATATTTTAACTATCCAGATTGTTTTAGCAATCTTTAGTATGATCTTTTTTCTTACTTTGCTATCTTTAATCTTGTTTTTGAGTTTGCAAAGTAATTTAGCTACAGCTTTAGTTGAAAACAGAAATAAAGCTGTGGAACTTGTAGATAACATTGTCTTTTTTTAA", "aa": "MVKRKRKPKLNSRNILTIQIVLAIFSMIFFLTLLSLILFLSLQSNLATALVENRNKAVELVDNIVFF", "aa_hexdigest": "3a71b4558c207c861345ffead1398d13", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B32C6", "ncbi_nrp_id": "WP_014894126.1", "uniref100_id": "UniRef100_UPI00027B32C6", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B32C6", "RefSeq:WP_014894126.1", "UniRef:UniRef100_UPI00027B32C6" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B32C6", "uniref90_id": "UniRef90_UPI00027B32C6" }, "psc": { "uniref90_id": "UniRef90_UPI00027B32C6", "uniref50_id": "UniRef50_UPI00027B32C6" }, "pscc": { "uniref50_id": "UniRef50_UPI00027B32C6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B32C6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7721.306799999995, "isoelectric_point": 11.0533784866333 }, "id": "JPCNHPFPBM_178", "locus": "GCA000292505_00139" }, { "type": "cds", "sequence": "contig_1", "start": 73688, "stop": 74578, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAAACCTAATTAAAAATGTTATTCGTTCTTTAAAGAGTGCTAAGATTGCTTTAATAGCGTTAACTTTTTTAATTTTTGTTGCTGTTGGTGGTTTTGTGTTGTTAAATAACACAGTTAATAATTTTAACGCCGCTTTTAACTATGTTACCCACACTGGTAAATTAAGTAATGCCATCATTAATGAACGTTATGACTTTGGTAAATTAGAGTTTCAAGAACAGACCAATAATTCTCAGAATAGTAGCGACAGTTTTACTTTAACTTTAACTAACGATTCAAGAACAAGTTTTATTAATAATGCTTTGAGAACTAACCCTTCTTTGTATGAAGGATTAGTAACCCAAACTTTTAGTTATCAAAACAAAACTGAAATGACTGAAAAAACCAATATAGTTAATCAGTCTAAAATTATTGCTGCTAACAATCTTAACAATGCATTAAGTAAAGATAAACAGCTCTTAGTTTCAGGTCAACTTGAAAAACTAAATGCTGTTTTTCGAGAATATAAAGCTATTAATATTACTGACAAAAGTGTTTTTAAAAAATTGATAGTATCAGAACCTAATGATTTGGTAAATAGCCTAGTTATTTTTGATGGTCAAAATTTATCTCTCTCCAAACAAAGTTATTTCAATAATTTTTTAAATCAATTTAACGAAATTAAATCAAAGGGTAAAGATAATTTAAGTACTACTTTAAAAACTGGGCAGTATCAGGCATTTTTACAAACTCTTTTTGATTATGTTCAAGCGAGTGAAACAACATTAAAAGATCAGCTTCAAAAGTTAATTTCAAATCCAGATTCAAGTAAAACAAATCAGGTTAAAAATCTCTTTGATACTCCAAGTACGCTTACTAATATTGGGGGTCAATTAACCTTACAATGA", "aa": "MLNLIKNVIRSLKSAKIALIALTFLIFVAVGGFVLLNNTVNNFNAAFNYVTHTGKLSNAIINERYDFGKLEFQEQTNNSQNSSDSFTLTLTNDSRTSFINNALRTNPSLYEGLVTQTFSYQNKTEMTEKTNIVNQSKIIAANNLNNALSKDKQLLVSGQLEKLNAVFREYKAINITDKSVFKKLIVSEPNDLVNSLVIFDGQNLSLSKQSYFNNFLNQFNEIKSKGKDNLSTTLKTGQYQAFLQTLFDYVQASETTLKDQLQKLISNPDSSKTNQVKNLFDTPSTLTNIGGQLTLQ", "aa_hexdigest": "dd1d5c948e7d4d1fcc277417c6f670a1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 33162.09130000004, "isoelectric_point": 9.267281913757323 }, "id": "JPCNHPFPBM_179", "locus": "GCA000292505_00140" }, { "type": "cds", "sequence": "contig_1", "start": 75083, "stop": 75391, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02687.27" ], "nt": "TTGAATTTACTAGCAGCAAGAACAGTTTTTATCCCTAATCTAATTAACACAATTAATTTAGTGGCTTTGTTTTTAACTATTGCTATCCTAACTGTTGCTATAATTGTCAGCATTTTAATCCTGATTAGTTATTTAAAGAAAAATACTGAGCAAATTGGCATTTTAAAAGCAAATGGGGTAAGTGGTAAAAAGATTAACCTTAGCTTGTTAATCTTTGGGTTAATTCCTGCTATAGTAGGTGCTATTTCTGGATATAGCTTTGGAATTGGATTTCAAGACGTAGCTATTCATCTATTTAGTAACTATTGA", "aa": "MNLLAARTVFIPNLINTINLVALFLTIAILTVAIIVSILILISYLKKNTEQIGILKANGVSGKKINLSLLIFGLIPAIVGAISGYSFGIGFQDVAIHLFSNY", "aa_hexdigest": "f7ed193e2eb671e79f0c9d2acf730926", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02687.27", "name": "FtsX", "length": 74, "aa_cov": 0.7156862745098039, "hmm_cov": 1.0, "evalue": 6.263944796863297e-11, "score": 37.527793884277344, "start": 24, "stop": 96 } ], "seq_stats": { "molecular_weight": 10929.07629999999, "isoelectric_point": 9.777226829528807 }, "id": "JPCNHPFPBM_180", "locus": "GCA000292505_00141" }, { "type": "cds", "sequence": "contig_1", "start": 75434, "stop": 75640, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTTTTTCACTGTTTGTTATCTTAATTATGAGTAGTATATCGCTTTTAGTGGGATCAATTATCTTAAAGAAGGATGTTGTAAAGATTTTAAAGCATGACAGTGAATTTAAAGTTTCAAGATTAGGACTTAGTTCTAAGAAATTGTTTGCTAGGTTTGGTATTATGACCAGGTTTAGAGTAGCATTAGCATTTAACGCTCCTTGA", "aa": "MFFSLFVILIMSSISLLVGSIILKKDVVKILKHDSEFKVSRLGLSSKKLFARFGIMTRFRVALAFNAP", "aa_hexdigest": "8beeb4b01f9c33f95dd04916f08798f9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7677.318799999996, "isoelectric_point": 11.13389949798584 }, "id": "JPCNHPFPBM_181", "locus": "GCA000292505_00142" }, { "type": "cds", "sequence": "contig_1", "start": 75662, "stop": 75814, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTCATTTACAATGATGATTTTAAACCTTAGTTTTGCAACTAAAGATAGCTTTGAAAATGCTCAATCAAAAACTAATTTAACTAATCAGAACCACCAATATGAATTTGAACTCGCTTCAGCAACAACACAAAGTGGTTTATTGAAGTGA", "aa": "MSSFTMMILNLSFATKDSFENAQSKTNLTNQNHQYEFELASATTQSGLLK", "aa_hexdigest": "7a78ee8852ef66890264e9313cd3a180", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5620.217699999999, "isoelectric_point": 5.510108375549315 }, "id": "JPCNHPFPBM_182", "locus": "GCA000292505_00143" }, { "type": "cds", "sequence": "contig_1", "start": 76076, "stop": 76570, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAACTAACCAAGCATCTGCTTCCAACACTGCTTTTCAAAATTTTTTAAAAGCAATCATTACTATAAATCCAAGCCAAGGATCGCAATTCATTAAACAAACCCAAGATCCATTAACAAAAAGATTTATCTATGCAATTGACAGTGATAAGGCATTAAAAAATAATAATGAACAAAACGGTTCCCAAAACCACTTAACTTTAAATGATGATTTTGCTAAATTTCTCTACAGTCAATTTGAATTAATTAAAAAGAGCGGGAATGCAAGTAATGAAGATTTAAATGCAATTGATTTTGAAAACCCCCAAACAATCAGAGATTTTTACAACAAGTACAATGCTTTACCACCATTAGATTACAAACTTAGTTTTAATGTAATAGGTTTACCCAAAGAGACAATTGCTGGACAAATTGACACCCCTAAGTATGGATTTTTAACCCTTTATGGTGAATATCAAAATACTCCTATCAAGATTAAAGGTATTAAAGATTGA", "aa": "MPTNQASASNTAFQNFLKAIITINPSQGSQFIKQTQDPLTKRFIYAIDSDKALKNNNEQNGSQNHLTLNDDFAKFLYSQFELIKKSGNASNEDLNAIDFENPQTIRDFYNKYNALPPLDYKLSFNVIGLPKETIAGQIDTPKYGFLTLYGEYQNTPIKIKGIKD", "aa_hexdigest": "cd8c1619c58c738ee6a2af3897974616", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18553.567899999987, "isoelectric_point": 6.582205390930175 }, "id": "JPCNHPFPBM_183", "locus": "GCA000292505_00144" }, { "type": "cds", "sequence": "contig_1", "start": 76580, "stop": 77635, "strand": "+", "frame": 2, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0011815B2F" ], "nt": "GTGGATAATTTAGGTCCAGTTTTGAGTGATCAAAACAACCACATTATTAATCAAGAATTGTTTAAAAATTATTCTTTTAATCCTTTGATAGTTAACAATTCTGCTGCAAAAAAATACCAACTTGCAATAGATAGTGAGATTAATATTGCAGTTAACAACAGCTTCAAACGGATTGACAATAAGATCATTAATCAAGATCCTTTAGTGAATGCTACCTTTAGAGTTGTAGGGATTAACAATTCCGCTCATGATCCTGAATTTTTCACTAGTTATAGTACTGCTTTTAAAGTATTGGAATATCCCAATGAATGGTTCGTAAAAAAACTTCCATTTAATAGCTTCTATGCTAATTCGCTTTTAAGTTTTGTTCAATCTACTTCGCTATTTTCTGAATCTGGTATTTTTCCTGCTACTAGTAGTTTTTCAACTAATAACACTGTACTTGTTGAGTTAATTAAAAAAACCATTAATTACAAGAATGGTCAAATGAATCAAACTTCAAGTAATGACTCTTCTAAGAAAGAAAATTACCAAAAATTGCAAAAAGCATTAGGAATATCAACTGATTTGGAGATTAGTAAAGTTAATGAATATGTTGCTATCTTAGCAAGGGTTTATAATGGTTTACCTTACAACTCTACTATTAGCTTTATTAGCAATGTTGCTGCTAACAACGCTTTATTTGGAAATATTGCTAACACCACCAAGCAGATTCAAGCTGTTGTAATTGCAGTGATTATTCCTATAATCATGTTGATTATTCTTTTGGTTTCAACTACCTTAATTCAAGAGTTGAAAAAAATTGCTATTAGATTAAAAGCATTGGGATATTCCAATTTAAAAATTCTCGCTTCATTTTTATCAATATACATCCCTTTATTTGCCTTTGGTTTGTTGATTTCTATCCCCTTTTCTATCTATCTAATTGCACTACATAATGAGGTAATTTTTGCAAGCTCATCGATCTTTTTAGATGCTTTTTTAAGTTTTGAAAGTGCAATTGGTTCAATGTTAGTTTTACTAGGGGTTTTATCAATTACCTTTATGTTGAATTGA", "aa": "MDNLGPVLSDQNNHIINQELFKNYSFNPLIVNNSAAKKYQLAIDSEINIAVNNSFKRIDNKIINQDPLVNATFRVVGINNSAHDPEFFTSYSTAFKVLEYPNEWFVKKLPFNSFYANSLLSFVQSTSLFSESGIFPATSSFSTNNTVLVELIKKTINYKNGQMNQTSSNDSSKKENYQKLQKALGISTDLEISKVNEYVAILARVYNGLPYNSTISFISNVAANNALFGNIANTTKQIQAVVIAVIIPIIMLIILLVSTTLIQELKKIAIRLKALGYSNLKILASFLSIYIPLFAFGLLISIPFSIYLIALHNEVIFASSSIFLDAFLSFESAIGSMLVLLGVLSITFMLN", "aa_hexdigest": "208f66d2fe7118e076efa8a74c9a00a6", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0011815B2F", "query_cov": 1.0427350427350428, "subject_cov": 0.8413793103448276, "identity": 0.617, "score": 389.0, "evalue": 1.14e-130, "valid": false, "product": "ABC transporter permease", "uniref50_id": "UniRef50_UPI0011815B2F" }, "pscc": { "uniref50_id": "UniRef50_UPI0011815B2F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0011815B2F" ], "product": "ABC transporter permease" }, "genes": [], "id": "JPCNHPFPBM_184", "locus": "GCA000292505_00145" }, { "type": "cds", "sequence": "contig_1", "start": 77683, "stop": 77883, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTTTTCTCTTTAAACAAAATCATAAAACCCAACCAGAAATTCACTAGTAATGAAGCTGAATTTCTACAGATAGCTACTGATTATTTGGAGGAAAGTCAAAACTATCTTCAAAAGGGTTTAAAGCAATTAAAAAAAGAATATAAAAGATCCATTATTTATAACCCTAACCTTGAATATAAACGCTTTGTTAAATGA", "aa": "MDFFSLNKIIKPNQKFTSNEAEFLQIATDYLEESQNYLQKGLKQLKKEYKRSIIYNPNLEYKRFVK", "aa_hexdigest": "98c702ed2232c26b335f4205013eaed0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8026.137799999998, "isoelectric_point": 9.397765922546387 }, "id": "JPCNHPFPBM_185", "locus": "GCA000292505_00146" }, { "type": "cds", "sequence": "contig_1", "start": 78394, "stop": 79077, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P9E0" ], "nt": "GTGTATAAATACATCACTAATGGCATTACTACAAATGCAGTTCTTAAAGGAGTTGATCTTGCCATTAAAAGTCATGATTTTATTGTGATTTTAGGCCCTTCAGGATCTGGTAAAACCACATTACTAAACATTATTTCAGGGATGGATAGAGCTTCTAGTGGTAGTGTTATTGTCAATGGTTATAACATGATCTGTTTAAATGATAGAAAGCTCACTAAATTCCGTCAAAAGTATGTTGGTTACATCTTTCAACAATACGGTTTATTACCTAATTTAACAGTTAGAGAAAACATTGAGATAGGAGCAAATCTTCAACCAGATCCTAGTAAAAGGATCAGCATTGATGCACTTTTAGAAGCGGTTGGGATGGATAGTTTGCAAAAGAAGCTTCCTAATGAATTGAGTGGTGGGCAACAGCAACGTGTTTCCATTGCAAGAGCTTTTGCTAAAAACCCCTTATTAATTTTTGGTGATGAACCTACTGGGGCACTTGATCTTGAGATGACCCAAATTGTTTTAAAACAGTTTTTAGCAATTAAAAAGCGTTATCAAACGACAATGATTATTGTTACCCACAACAATTTAATTGCTAACTTAGCTGATTTAGTTATCTATGTAGCAGATGGAAAAATAAAATCACTACACAGGAACTTAAATCCTAAACAGGTTGAAGAGATCCATTGA", "aa": "MYKYITNGITTNAVLKGVDLAIKSHDFIVILGPSGSGKTTLLNIISGMDRASSGSVIVNGYNMICLNDRKLTKFRQKYVGYIFQQYGLLPNLTVRENIEIGANLQPDPSKRISIDALLEAVGMDSLQKKLPNELSGGQQQRVSIARAFAKNPLLIFGDEPTGALDLEMTQIVLKQFLAIKKRYQTTMIIVTHNNLIANLADLVIYVADGKIKSLHRNLNPKQVEEIH", "aa_hexdigest": "bc7686ff677e6c1249c40cc7f03caf28", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P9E0", "query_cov": 0.8766519823788547, "subject_cov": 0.9900497512437811, "identity": 0.698, "score": 267.0, "evalue": 3.76e-88, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A3B0P9E0" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P9E0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P9E0" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "JPCNHPFPBM_186", "locus": "GCA000292505_00147" }, { "type": "cds", "sequence": "contig_1", "start": 79087, "stop": 79938, "strand": "+", "frame": 1, "gene": "tkt", "product": "transketolase", "db_xrefs": [ "COG:COG0021", "COG:G", "EC:2.2.1.1", "GO:0004802", "GO:0005829", "GO:0006098", "GO:0046872", "KEGG:K00615", "SO:0001217", "UniRef:UniRef50_P75611", "UniRef:UniRef90_P47312" ], "nt": "ATGAAATACTTATATGCCACTCAACACCTTACTTTAAATGCTATTAAGCATGCTAAGGGAGGACATGTTGGCATGGCCATTGGTGCAAGTCCTATCTTATTTAGTTTATTTACTAAACACTTTCACTTTGATCCTGACCAACCAAAGTGGATCAACAGAGATCGCTTTGTTTTAAGTGCTGGCCATGGTAGCATGGCATTATATTCAATTTTCCATTTTGCCGGACTTATTTCTAAACAAGAGATCTTACAGCATAAACATGGTCAAATTAACACTTCTTCCCATCCTGAATATGCTCCAAATAACTTCATAGATGCATCAACAGGCCCTTTAGGTCAAGGCTTTGGCATGGCAGTTGGCATGGCGTTAGCACAAAAGTTATTAGCTAATGAATTTAAAGAGCTAAGTGATAAATTGTTTGACCATTACACCTATGTGGTTGTTGGGGATGGAGATCTACAGGAGGGGGTTAGTTATGAAGTTAGTCAAATTGCTGGGTTATATAAATTAAATAAACTAATTGTGCTTCATGATTCAAATAGAGTGCAAATGGATAGTGAAGTAAAAAAAGTTGCTAATGAAAATCTAAAGGTTAGGTTTGAAAACGTTGGTTGGAATTACATCCATACTGATGATCAACTAGAAAATATTGATCAAGCTATTATTAAAGCCAAACAATCAGATAAGCCAACTTTTATTGAAGTGAGAACAACTATTGCTAAAAACACCCACCTTGAAGATCAGTATGGAGGACATTGGTTTATTCCCAATGAAGTGGACTTTCAACTTTTTGAGAAAAGAACAAATACTAACTTTAACTTTTTTAATTATCCAGATAGTATTTACCAATGA", "aa": "MKYLYATQHLTLNAIKHAKGGHVGMAIGASPILFSLFTKHFHFDPDQPKWINRDRFVLSAGHGSMALYSIFHFAGLISKQEILQHKHGQINTSSHPEYAPNNFIDASTGPLGQGFGMAVGMALAQKLLANEFKELSDKLFDHYTYVVVGDGDLQEGVSYEVSQIAGLYKLNKLIVLHDSNRVQMDSEVKKVANENLKVRFENVGWNYIHTDDQLENIDQAIIKAKQSDKPTFIEVRTTIAKNTHLEDQYGGHWFIPNEVDFQLFEKRTNTNFNFFNYPDSIYQ", "aa_hexdigest": "c9eaae64a7b9f4d7e3665cfe1b198bca", "start_type": "ATG", "rbs_motif": null, "genes": [ "tkt" ], "truncated": "3-prime", "pseudogene": { "start": 79087, "stop": 80241, "observations": { "mutation": [ 79936 ], "stop": [ 79936 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47312", "query_cov": 0.9964664310954063, "subject_cov": 0.4351851851851852, "identity": 0.996, "score": 577.0, "evalue": 1.33e-202, "gene_start": 1, "gene_end": 282, "reference_start": 1, "reference_end": 282, "reference_sequence": "MKYLYATQHLTLNAIKHAKGGHVGMAIGASPILFSLFTKHFHFDPDQPKWINRDRFVLSAGHGSMALYSIFHFAGLISKQEILQHKHGQINTSSHPEYAPNNFIDASTGPLGQGFGMAVGMVLAQKLLANEFKELSDKLFDHYTYVVVGDGDLQEGVSYEVSQIAGLYKLNKLIVLHDSNRVQMDSEVKKVANENLKVRFENVGWNYIHTDDQLENIDQAIIKAKQSDKPTFIEVRTTIAKNTHLEDQYGGHWFIPNEVDFQLFEKRTNTNFNFFNYPDSIYHWFKQTVIERQKQIKEDYNNLLISLKDKPLFKKFTNWIDSDFQALYLNQLDEKKVAKKDSATRNYLKDFLNQINNPNSNLYCLNADVSRSCFIKIGDDNLHENPCSRNIQIGIREFAMATIMNGMALHGGIKVMGGTFLAFADYSKPAIRLGALMNLPVFYVYTHDSYQVGGDGPTHQPYDQLPMLRAIENVCVFRPCDEKETCAGFNYGLLSQDQTTVLVLTRQPLKSIDNTDSLKTLKGGYILLDRKQPDLIIAASGSEVQLAIEFEKVLTKQNVKVRILSVPNITLLLKQDEKYLKSLFDANSSLITIEASSSYEWFCFKKYVKNHAHLGAFSFGESDDGDKVYQQKGFNLERLMKIFTSLRN" }, "paralog": false, "identity": 0.9895833333333334, "score": 776.0, "evalue": 1.41e-283, "description": "Internal stop codon at 79936. Nonsense mutation around 79936.", "psc": { "uniref90_id": "UniRef90_P47312", "gene": "tkt", "product": "transketolase", "ec_ids": [ "2.2.1.1" ], "uniref50_id": "UniRef50_P75611", "cog_id": "COG0021", "cog_category": "G", "kegg_orthology_id": "K00615", "go_ids": [ "GO:0005829", "GO:0046872", "GO:0004802", "GO:0006098" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47312", "EC:2.2.1.1", "UniRef:UniRef50_P75611", "COG:COG0021", "COG:G", "KEGG:K00615", "GO:0005829", "GO:0046872", "GO:0004802", "GO:0006098" ] }, "pscc": { "uniref50_id": "UniRef50_P75611", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75611" ], "product": "Transketolase" } }, "id": "JPCNHPFPBM_187", "locus": "GCA000292505_00148" }, { "type": "cds", "sequence": "contig_1", "start": 79993, "stop": 81033, "strand": "+", "frame": 1, "gene": "tkt", "product": "transketolase", "db_xrefs": [ "COG:COG0021", "COG:G", "EC:2.2.1.1", "GO:0004802", "GO:0005829", "GO:0006098", "GO:0046872", "KEGG:K00615", "SO:0001217", "UniRef:UniRef50_P75611", "UniRef:UniRef90_P47312" ], "nt": "TTGCTAATTTCTCTTAAAGACAAACTACTTTTTAAAAAATTTACTAATTGGATTGACAGTGATTTTCAAGCCCTTTATCTTAACCAACTAGATGAAAAGAAAGTAGCAAAAAAAGATAGTGCTACTAGAAACTATTTAAAAGATTTTTTAAACCAAATTAATAATCCTAATTCCAACTTGTATTGCTTAAATGCTGATGTATCACGTTCTTGTTTTATCAAGATAGGTGATGATAATCTCCATGAAAATCCTTGTTCTAGAAATATCCAAATAGGAATTAGGGAGTTTGCAATGGCAACAATAATGAATGGTATGGCACTTCATGGTGGTATTAAAGTGATGGGTGGTACTTTTTTAGCATTTGCTGATTATTCAAAGCCAGCAATTCGCTTAGGTGCATTAATGAACTTACCAGTATTTTATGTTTATACCCATGACTCTTATCAAGTAGGGGGTGATGGTCCTACTCATCAACCCTATGATCAACTACCAATGTTAAGAGCAATTGAAAATGTTTGTGTATTTCGTCCTTGTGATGAAAAGGAAACTTGTGCTGGATTTAACTATGGTCTTTTAAGTCAAGATCAGACAACTGTTTTGGTTTTAACACGTCAACCCTTAAAATCCATTGATAACACTGATAGTTTAAAAACACTGAAGGGTGGTTATATCCTTTTGGATAGAAAACAACCTGATTTAATTATTGCTGCTAGTGGTAGTGAAGTGCAACTTGCAATAGAGTTTGAAAAAGTTTTAACTAAACAAAATGTAAAGGTAAGAATTCTGTCAGTTCCCAATATAACTTTACTTTTAAAACAAGATGAAAAATATCTAAAGAGTTTATTTGATGCTAACAGTTCACTTATCACCATAGAAGCTAGTAGTAGCTATGAGTGGTTTTGCTTTAAGAAGTATGTTAAAAACCATGCTCATTTAGGAGCTTTTAGTTTTGGTGAATCTGATGATGAAGATAAAGTTTATCAGCAAAAAGGGTTTAATCTGGAAAGGTTAATGAAAATATTTACTTCCCTAAGAAATTAA", "aa": "MLISLKDKLLFKKFTNWIDSDFQALYLNQLDEKKVAKKDSATRNYLKDFLNQINNPNSNLYCLNADVSRSCFIKIGDDNLHENPCSRNIQIGIREFAMATIMNGMALHGGIKVMGGTFLAFADYSKPAIRLGALMNLPVFYVYTHDSYQVGGDGPTHQPYDQLPMLRAIENVCVFRPCDEKETCAGFNYGLLSQDQTTVLVLTRQPLKSIDNTDSLKTLKGGYILLDRKQPDLIIAASGSEVQLAIEFEKVLTKQNVKVRILSVPNITLLLKQDEKYLKSLFDANSSLITIEASSSYEWFCFKKYVKNHAHLGAFSFGESDDEDKVYQQKGFNLERLMKIFTSLRN", "aa_hexdigest": "30855aaa5a2178acd9a9de8587f1b7d0", "start_type": "TTG", "rbs_motif": null, "genes": [ "tkt" ], "truncated": "5-prime", "pseudogene": { "start": 79693, "stop": 81033, "observations": { "mutation": [ 79936 ], "stop": [ 79936 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47312", "query_cov": 1.0, "subject_cov": 0.5339506172839507, "identity": 0.991, "score": 690.0, "evalue": 2.7e-246, "gene_start": 1, "gene_end": 346, "reference_start": 303, "reference_end": 648, "reference_sequence": "MKYLYATQHLTLNAIKHAKGGHVGMAIGASPILFSLFTKHFHFDPDQPKWINRDRFVLSAGHGSMALYSIFHFAGLISKQEILQHKHGQINTSSHPEYAPNNFIDASTGPLGQGFGMAVGMVLAQKLLANEFKELSDKLFDHYTYVVVGDGDLQEGVSYEVSQIAGLYKLNKLIVLHDSNRVQMDSEVKKVANENLKVRFENVGWNYIHTDDQLENIDQAIIKAKQSDKPTFIEVRTTIAKNTHLEDQYGGHWFIPNEVDFQLFEKRTNTNFNFFNYPDSIYHWFKQTVIERQKQIKEDYNNLLISLKDKPLFKKFTNWIDSDFQALYLNQLDEKKVAKKDSATRNYLKDFLNQINNPNSNLYCLNADVSRSCFIKIGDDNLHENPCSRNIQIGIREFAMATIMNGMALHGGIKVMGGTFLAFADYSKPAIRLGALMNLPVFYVYTHDSYQVGGDGPTHQPYDQLPMLRAIENVCVFRPCDEKETCAGFNYGLLSQDQTTVLVLTRQPLKSIDNTDSLKTLKGGYILLDRKQPDLIIAASGSEVQLAIEFEKVLTKQNVKVRILSVPNITLLLKQDEKYLKSLFDANSSLITIEASSSYEWFCFKKYVKNHAHLGAFSFGESDDGDKVYQQKGFNLERLMKIFTSLRN" }, "paralog": false, "identity": 0.9910313901345291, "score": 895.0, "evalue": 0.0, "description": "Internal stop codon at 79936. Nonsense mutation around 79936.", "psc": { "uniref90_id": "UniRef90_P47312", "gene": "tkt", "product": "transketolase", "ec_ids": [ "2.2.1.1" ], "uniref50_id": "UniRef50_P75611", "cog_id": "COG0021", "cog_category": "G", "kegg_orthology_id": "K00615", "go_ids": [ "GO:0005829", "GO:0046872", "GO:0004802", "GO:0006098" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47312", "EC:2.2.1.1", "UniRef:UniRef50_P75611", "COG:COG0021", "COG:G", "KEGG:K00615", "GO:0005829", "GO:0046872", "GO:0004802", "GO:0006098" ] }, "pscc": { "uniref50_id": "UniRef50_P75611", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75611" ], "product": "Transketolase" } }, "id": "JPCNHPFPBM_188", "locus": "GCA000292505_00149" }, { "type": "cds", "sequence": "contig_1", "start": 81083, "stop": 81292, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "ATGCCTGGTTGCATCTTACTTTCATCCTGTTCTTTTAGAAGTTATATCCCAACTCCTAGTTTAAGAAATACTGTTGGTAACCACAACAGTTATGTTAATAATACTGTCCCTAAAAACAATTTTTATGAAAAGTTTTATGATCTAACTTTTGCTTTAAATTTCACTAATCAGAAAACTCAAGAGTTTGGTACTGGTTGGTTAATTGACTGA", "aa": "MPGCILLSSCSFRSYIPTPSLRNTVGNHNSYVNNTVPKNNFYEKFYDLTFALNFTNQKTQEFGTGWLID", "aa_hexdigest": "fc002f78baf29bb0d383dafd8541c01e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 39, "aa_cov": 0.4492753623188406, "hmm_cov": 1.0, "evalue": 4.842561686135136e-08, "score": 28.07671546936035, "start": 39, "stop": 69 } ], "seq_stats": { "molecular_weight": 7916.801200000002, "isoelectric_point": 7.786185264587402 }, "id": "JPCNHPFPBM_189", "locus": "GCA000292505_00150" }, { "type": "cds", "sequence": "contig_1", "start": 81527, "stop": 81712, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTAGGTTTGCCAATCCTAAGATAAATTCCAAACCAGAAGAGATGAACTACCAAGTTCAAACTCGTTTAAAACAAGATGCAATGGTGTTTATCCAAACCAGTACTTTACCTAAAACTGCTTATGCAGGAATTGATCCTATTAACTTTGATTACCATGAAACTAGTGATGAGAGTGGATTTTGA", "aa": "MGRFANPKINSKPEEMNYQVQTRLKQDAMVFIQTSTLPKTAYAGIDPINFDYHETSDESGF", "aa_hexdigest": "ae25d8c8c9459c672b3e39e998832611", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6972.7134, "isoelectric_point": 5.082394981384277 }, "id": "JPCNHPFPBM_190", "locus": "GCA000292505_00151" }, { "type": "cds", "sequence": "contig_1", "start": 82618, "stop": 84042, "strand": "+", "frame": 1, "gene": null, "product": "putative lipoprotein MG068", "db_xrefs": [ "GO:0005886", "RefSeq:WP_014894553.1", "SO:0001217", "UniParc:UPI00027B32CA", "UniRef:UniRef100_UPI00027B32CA", "UniRef:UniRef50_P47314", "UniRef:UniRef90_P47314" ], "nt": "GTGTTAAGACGTTATCTAACTCTGAGTTTTTCTAGCTTGCTTTTATTAGCACTGCTGTTTTTAACAGGCTGTTCTTTTGTTAGACCACAATTTCGCAGGGGCTTTAGAACCCAGTTTAAAATTAACTCTATTCCAACTGTTAGTGATCCTTATCACATTAACTACGACTTAACTTTTTCATTAAACTTTGCTTCCAACAAAAGAAATACTTATGGTACTGGTTGGTTAATTGATTGGAAAGGAGATGAAAATAACCCTGAGAAAAATGATCCTTTTAAAGTTTATTTAGCTACTAACCTCCATGTGATAGATGCTTTAAGAAATAATAATGACTATGAACCATATAACAAGGATAGTAATAACCAAGCTTTTAATGGCGAAGAGATCACTAGGTTCTTTTCTATAGGTAAGTACACATATCCTAGTATTTTTAGTGAATTAAATTTCATTAGCAATGCTAGAGAAGCGTTTGTTTCGATTCAAACCTCTACTATTCCTAAAACTGCTTATGCTGCTGTTAACTTTGTTGAAACACAAGGAGAGGATGAAAGTTATACAGATTCATTATCAACTGATAATAAAAGAGATATTTATGCGGATTTTGCTGTGATAGAAATCCCCTTATTCTTAACTAACCATCGCGATTATCAAGTATTTAATGAATTTATTAAACCAGCAATAGAAACATACAAACAACTAGGAAACTCTTCCTTTGAAAAAAAACAACTAGATCAACATAAAAACGACAACTTTTACATGTTAGGTTATCCTTTGGTTGAGAGTAGCATAGATGCTCTAATTTTGAACCAAAGAAGACAGTACAATAACAGTTATACTGAAAAATATACTCCTCAAACTTTAACCAAAGATCAACGTACCATTGACTTAAGCAGAGAAGTTCCTACTTTAATTCAGAACAAAACAGAAAACTCTACAGGAAGTCAATTATTAGTAAATCAATCTTTAAGTAGTACATCTGAGGGGATTATTGAATTTATTAAGTTACCTGAATTTAAACTCAATTATCATAATAAAAGTTATCGTCAATATGGTAGAGGTTTTGCTCTACAAAACACTAACTTTAGACCAGGTTCTTCAGGAACTTTAATGTTAAATAACCAAAAACAGATAGCAGGTATTTATTTTGGTGTTTTAGATTTTGGAGAAGATGTAAGTTTAATGAGTAACATTGGTGTTGGACAAATTCTTCGTGTTCCTCAAAAGAGCAATACAAAAAATAGATCAATTGCTACTAATAAAAGCAACTATGATTTGATTTTTGGTGATGGCAATACAACTAACTTTTATGCAAAATTTGCTAGACAAAATAACACCCATCTTTATCAGATGATTTCAAATAGTAAAGATACAAAATTAAAGTATGTGAATACTGTTGAAAAAACAGTAAAAGCTAGTATTAAATAA", "aa": "MLRRYLTLSFSSLLLLALLFLTGCSFVRPQFRRGFRTQFKINSIPTVSDPYHINYDLTFSLNFASNKRNTYGTGWLIDWKGDENNPEKNDPFKVYLATNLHVIDALRNNNDYEPYNKDSNNQAFNGEEITRFFSIGKYTYPSIFSELNFISNAREAFVSIQTSTIPKTAYAAVNFVETQGEDESYTDSLSTDNKRDIYADFAVIEIPLFLTNHRDYQVFNEFIKPAIETYKQLGNSSFEKKQLDQHKNDNFYMLGYPLVESSIDALILNQRRQYNNSYTEKYTPQTLTKDQRTIDLSREVPTLIQNKTENSTGSQLLVNQSLSSTSEGIIEFIKLPEFKLNYHNKSYRQYGRGFALQNTNFRPGSSGTLMLNNQKQIAGIYFGVLDFGEDVSLMSNIGVGQILRVPQKSNTKNRSIATNKSNYDLIFGDGNTTNFYAKFARQNNTHLYQMISNSKDTKLKYVNTVEKTVKASIK", "aa_hexdigest": "6c545bba48db750518a2fe918f2fbfce", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B32CA", "ncbi_nrp_id": "WP_014894553.1", "uniref100_id": "UniRef100_UPI00027B32CA", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B32CA", "RefSeq:WP_014894553.1", "UniRef:UniRef100_UPI00027B32CA" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B32CA", "uniref90_id": "UniRef90_P47314" }, "psc": { "uniref90_id": "UniRef90_P47314", "product": "Uncharacterized lipoprotein MG068", "uniref50_id": "UniRef50_P47314", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47314", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47314" ], "product": "Uncharacterized lipoprotein MG068" }, "genes": [], "id": "JPCNHPFPBM_191", "locus": "GCA000292505_00152" }, { "type": "cds", "sequence": "contig_1", "start": 84529, "stop": 84702, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAAATGCTAATACACTAATAACCACTAAGAGTGTTATTAATATTGATCACAATAAAACTAAAGTTGTTGTTAGAAATTTAACTTTACTTGTGTCTATTTCATTTAAACAAGTCTTTTTATCATTTGAAATATTTAATTCTTTTTGCACTTTCCAACTAATAAAATAA", "aa": "MKLNANTLITTKSVINIDHNKTKVVVRNLTLLVSISFKQVFLSFEIFNSFCTFQLIK", "aa_hexdigest": "6992b0bfba3b913ebfcb4964c7b2bac0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6561.753799999997, "isoelectric_point": 10.022400093078613 }, "id": "JPCNHPFPBM_192", "locus": "GCA000292505_00153" }, { "type": "cds", "sequence": "contig_1", "start": 84833, "stop": 85048, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAATTGTTGGAATTCACAGCTTTTTAGTTGGAATTTTAGTTAATCATCAAACACTTAAAATAAGTAAAAAGTATGTTATTTTAGGTTCGATTTTTCCAATTATGGCATTAACAAATACTCTTGTAATTAGAAAAAAATTAAAAGCTTTATTAGGAGAGGGTAAGGTTCAAAAAGGACTCAAAGTTTTAACTATTAGTTTTATTTGTGTTTGA", "aa": "MAIVGIHSFLVGILVNHQTLKISKKYVILGSIFPIMALTNTLVIRKKLKALLGEGKVQKGLKVLTISFICV", "aa_hexdigest": "06a22ba146000966bef1f619ce3f5443", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7760.576499999999, "isoelectric_point": 10.37175426483154 }, "id": "JPCNHPFPBM_193", "locus": "GCA000292505_00154" }, { "type": "cds", "sequence": "contig_1", "start": 85316, "stop": 85552, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED97BE8", "UniRef:UniRef90_UPI001ED97BE8" ], "nt": "TTGGCTTTAACCTTAATTGATTTAATGGGTTTAATTTTGGCTTTTGGTATTATTGTTGTTAACAATACAAATTCTTCAAGTTTTGATGCAAAATCAAATTCCTTAGCATTAATTTTTAAGTTCATTTATTTTGATAGTTCTATAGCAGCCTTTTTAACAGTTTTAAGCTTTGCTAGTTCAGTTGCTTTACTTGTCAATTTAATAAAAAAAGATAAGGGATTAACCTTAAAAAATTAG", "aa": "MALTLIDLMGLILAFGIIVVNNTNSSSFDAKSNSLALIFKFIYFDSSIAAFLTVLSFASSVALLVNLIKKDKGLTLKN", "aa_hexdigest": "bcd56ee307a3d42b1e867789222c4fdd", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 85052, "stop": 85552, "observations": { "mutation": [ 85079, 85223, 85289 ], "stop": [ 85079, 85223, 85289 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001ED97BE8", "query_cov": 1.0, "subject_cov": 0.46987951807228917, "identity": 0.9620000000000001, "score": 130.0, "evalue": 4.56e-37, "gene_start": 1, "gene_end": 78, "reference_start": 89, "reference_end": 166, "reference_sequence": "MQLIIGFFVWLFPYAGEAGINIGINLSIFNLAQLVGSDINVITYFTTFLTLLFAFLSWAVLHVLACFILVHLTIYKQQEWIKIKAIYILALTLIDLMGLILAFGIIVVNNTNSSSVDAKSNSLALIFKFIYFGSSIAAFLTVLSFASSVALLVNLIKKDKGLTLKN" }, "paralog": false, "identity": 0.963855421686747, "score": 296.0, "evalue": 5.28e-105, "description": "Internal stop codon at 85079, 85223, 85289. Nonsense mutation around 85079, 85223, 85289.", "psc": { "uniref90_id": "UniRef90_UPI001ED97BE8", "uniref50_id": "UniRef50_UPI001ED97BE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001ED97BE8", "UniRef:UniRef50_UPI001ED97BE8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001ED97BE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED97BE8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8387.883999999991, "isoelectric_point": 9.224861717224119 }, "id": "JPCNHPFPBM_194", "locus": "GCA000292505_00155" }, { "type": "cds", "sequence": "contig_1", "start": 85577, "stop": 85933, "strand": "+", "frame": 2, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7M3VDH2", "UniRef:UniRef90_UPI000314D47C" ], "nt": "GTGTTACTAGTGCCTAAGGTGGTAGTGGAGTACCACAAGCTCAGTAAGAAGGTTGTCAAAGAGAGTTTGGAAGTGGAAGCATCGTTCCACTTCAACCCCACCCAAAGGTTGAAGAAAGATAGTCCAGTGAAGGATTCAACAAAAACAGGGGAGAAACTCGAAACAACTGCTTCATCCATGAGTAGTGGTGGGGCTACATCCACTCGCAAGGCCCTCAAGGTTGAGGTGGAGAAACAAAGTGGATCAACTGATTCACTTTTAAAAAACGACTTTGCCAAAAAGCCACTGAAACATAAAGAAAATAGTGGGGAAGTGAAGTTAGATGCGAGTGGGGAGTTTGCGAAGGACAAGGCCTGA", "aa": "MLLVPKVVVEYHKLSKKVVKESLEVEASFHFNPTQRLKKDSPVKDSTKTGEKLETTASSMSSGGATSTRKALKVEVEKQSGSTDSLLKNDFAKKPLKHKENSGEVKLDASGEFAKDKA", "aa_hexdigest": "373789ba5db09fc88cf6e3ca8e730b7a", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000303F145", "query_cov": 0.9830508474576272, "subject_cov": 0.8529411764705882, "identity": 0.853, "score": 167.0, "evalue": 3.3e-51, "valid": false, "product": "adhesin", "uniref50_id": "UniRef50_A0A7M3VDG5" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 85586, "stop": 86146, "observations": { "mutation": [ 85931, 86129 ], "stop": [ 85931, 86129 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000314D47C", "query_cov": 0.9661016949152542, "subject_cov": 0.6129032258064516, "identity": 1.0, "score": 207.0, "evalue": 2.42e-66, "gene_start": 5, "gene_end": 118, "reference_start": 2, "reference_end": 115, "reference_sequence": "MPKVVVEYHKLSKKVVKESLEVEASFHFNPTQRLKKDSPVKDSTKTGEKLETTASSMSSGGATSTRKALKVEVEKQSGSTDSLLKNDFAKKPLKHKENSGEVKLDASGEFAKDKAWKPVLTTDEIKKNRGMGATVVSFSPESAMVKLFPTPPPLQLLLLLLLLLLLPPSPLFLTSMLGLNQWSLNT" }, "paralog": false, "identity": 0.9838709677419355, "score": 322.0, "evalue": 2.11e-114, "description": "Internal stop codon at 85931, 86129. Nonsense mutation around 85931, 86129.", "psc": { "uniref90_id": "UniRef90_UPI000314D47C", "product": "adhesin", "uniref50_id": "UniRef50_A0A7M3VDH2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000314D47C", "UniRef:UniRef50_A0A7M3VDH2" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7M3VDH2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7M3VDH2" ], "product": "MgpB adhesin protein" } }, "id": "JPCNHPFPBM_195", "locus": "GCA000292505_00156" }, { "type": "cds", "sequence": "contig_1", "start": 85992, "stop": 86135, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCATTGATTTAACCCCAACATTGATGTTAGAAAAAGTGGGGAGGGGGGTAGAAGAAGAAGCAGAAGTAGAAGAAGAAGAAGTTGAAGGGGAGGGGGAGTTGGAAAAAGCTTTACCATTGCCGATTCAGGGGAGAAACTAA", "aa": "MIIDLTPTLMLEKVGRGVEEEAEVEEEEVEGEGELEKALPLPIQGRN", "aa_hexdigest": "d067de2c03868e3734e69cdc8662df3c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5220.832599999999, "isoelectric_point": 4.0552576065063475 }, "id": "JPCNHPFPBM_196", "locus": "GCA000292505_00157" }, { "type": "cds", "sequence": "contig_1", "start": 86257, "stop": 86616, "strand": "+", "frame": 1, "gene": null, "product": "Random genomic clone esh10, partial cds", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49206" ], "nt": "GTGAAGCCTAGCAATAGTAGTAGTAGCAACACGTTTAATCCCAATGATGATGAAAATCAAGTCACTCCAGGTGGCTCCTCCAAACCAACCACCTACACCCATTTACCCAACAGTATCAGTCCCACCAGTGACTGGATCAATGCGTTGACTTTCACCAATAAGAATAATCCACAACGCAATCAGTTGTTGCTGAGAGCACTGTTAGGAACTATCCCGGTATTGATCAATAAGAGTGGAGGAAGTGGGAATGAGTTTAACCATACGAATGATCAGAAGTGGGATAAAACAGATAAAAAAGATGGCAACCTCCCGGGGTTTGGGGAGGTGAATGGTGGTTTTTACCCAGTTTTACTTATTTAA", "aa": "MKPSNSSSSNTFNPNDDENQVTPGGSSKPTTYTHLPNSISPTSDWINALTFTNKNNPQRNQLLLRALLGTIPVLINKSGGSGNEFNHTNDQKWDKTDKKDGNLPGFGEVNGGFYPVLLI", "aa_hexdigest": "b5f11b2d3d00ac03e271e39116c57f68", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030D20171", "query_cov": 1.0, "subject_cov": 1.0084745762711864, "identity": 0.866, "score": 207.0, "evalue": 1.92e-67, "valid": false, "product": "adhesin", "uniref50_id": "UniRef50_Q49206" }, "pscc": { "uniref50_id": "UniRef50_Q49206", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49206" ], "product": "Random genomic clone esh10, partial cds" }, "genes": [], "id": "JPCNHPFPBM_197", "locus": "GCA000292505_00158" }, { "type": "cds", "sequence": "contig_1", "start": 86821, "stop": 87030, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGTTTAGCACAGTTAGACAGTTCCTATCAAATCTCCGACCAAACCATCCATAACACCAACTTGTTTGTGTTGTTCAAGTCTACTCAGGTAAAAGTTAAGTATGACAGTTCAAGTGGCTCAAATAACCAGATTAGTTTTGATTCAACTAACAACAAACCATCCTACATCGTTGAGTTTACTAACTCTACCAAGATAGGCATCAAGTGA", "aa": "MSLAQLDSSYQISDQTIHNTNLFVLFKSTQVKVKYDSSSGSNNQISFDSTNNKPSYIVEFTNSTKIGIK", "aa_hexdigest": "5e3b59b489c9b0954bc06bf4985d39dd", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7711.450999999999, "isoelectric_point": 8.028779792785645 }, "id": "JPCNHPFPBM_198", "locus": "GCA000292505_00159" }, { "type": "cds", "sequence": "contig_1", "start": 87037, "stop": 87210, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13", "UniRef:UniRef90_UPI0030D43D23" ], "nt": "GTGAAAAAGTATCAGTTAGATGTGCCAAGTGTTTCAACAACCATGAACCAAGTTTTGCAAGAATTGATCCTTGAACAACCTTTGACTAAATATACCTTAAATAGTAGTTTGGCCAAACAAAAGGGCAAAACACAAGCAGTGGTGCATCTTGGTTCAAATATGGCAAATCAGTGA", "aa": "MKKYQLDVPSVSTTMNQVLQELILEQPLTKYTLNSSLAKQKGKTQAVVHLGSNMANQ", "aa_hexdigest": "fdc55e62904eee4b3d68db8733fd6ebe", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 86911, "stop": 87275, "observations": { "deletion": [ 87232 ], "mutation": [ 87028, 87208 ], "stop": [ 87028, 87208 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0030D43D23", "query_cov": 1.0, "subject_cov": 0.47107438016528924, "identity": 0.807, "score": 88.6, "evalue": 1.9e-21, "gene_start": 1, "gene_end": 57, "reference_start": 43, "reference_end": 99, "reference_sequence": "MKLTYSSSGSNTNTISFENNANKPSYVVEFTNSTNVGIKWRVVKKYQLDVPNVSSDMNQVLQELILEQPLTKYTLNSSLAKQKGKSKIEVHLGSGRATQWTSRTQLHWPKQQSQPKCINRI" }, "paralog": false, "identity": 0.6776859504132231, "score": 154.0, "evalue": 7.27e-50, "description": "Internal stop codon at 87028, 87208. Frameshift due to deletion around 87232. Nonsense mutation around 87028, 87208.", "psc": { "uniref90_id": "UniRef90_UPI0030D43D23", "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0030D43D23", "UniRef:UniRef50_UPI00027B3D13" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6347.299100000002, "isoelectric_point": 9.523801612854005 }, "id": "JPCNHPFPBM_199", "locus": "GCA000292505_00160" }, { "type": "cds", "sequence": "contig_1", "start": 87217, "stop": 87318, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTAATCAACACAACTTAGACAACAATCCCAGTCCCAATGCATCAACCGGATTTAAACTCACTACCGGCAATGCATATAGAAAACTAAGTGAGTCCTGA", "aa": "MRNQHNLDNNPSPNASTGFKLTTGNAYRKLSES", "aa_hexdigest": "34b1d72f7b0e72269bfe3a5424493d42", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3663.9423000000006, "isoelectric_point": 9.69399814605713 }, "id": "JPCNHPFPBM_200", "locus": "GCA000292505_00161" }, { "type": "cds", "sequence": "contig_1", "start": 87397, "stop": 87597, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FCDF660", "UniRef:UniRef90_UPI00027B39A0" ], "nt": "ATGGCAAAAAATGATGCGCCCAGTGTTACTAGAGGGAGAACATCAGATCAAAGTAATAAATTCACCAAGTACCTCAACACCAAACAAGCGTTAGAAAGCATCGGCATTTTGTTTGATGGGGATGGAATGAGGAATGTTGTTAGCCTCCTTCCTCTACTTTCAACCCAACAAGGTGAAAAGTGGTCAATACCAAACCACTAA", "aa": "MAKNDAPSVTRGRTSDQSNKFTKYLNTKQALESIGILFDGDGMRNVVSLLPLLSTQQGEKWSIPNH", "aa_hexdigest": "79f037f93a3c55ba762851865618119f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 87235, "stop": 87642, "observations": { "mutation": [ 87316, 87595, 87616, 87622 ], "stop": [ 87316, 87595, 87616, 87622 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00027B39A0", "query_cov": 0.9848484848484849, "subject_cov": 0.48148148148148145, "identity": 0.815, "score": 100.0, "evalue": 7.98e-26, "gene_start": 2, "gene_end": 66, "reference_start": 58, "reference_end": 120, "reference_sequence": "MGLNNNPSPNATTGFKLNKGNAYRKLNESWPIYQPIDGTQHGKGKDSSGWSSTEATTAKNDAPLSTGGGSSSGTFNKYLNTKQALERIGILFDGDGTRNVVSLLPLLSTQQGEKWSIPNHQHLQQVNWTWQVTIK" }, "paralog": false, "identity": 0.8, "score": 207.0, "evalue": 1.03e-70, "description": "Internal stop codon at 87316, 87595, 87616, 87622. Nonsense mutation around 87316, 87595, 87616, 87622.", "psc": { "uniref90_id": "UniRef90_UPI00027B39A0", "uniref50_id": "UniRef50_UPI002FCDF660", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00027B39A0", "UniRef:UniRef50_UPI002FCDF660" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FCDF660", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FCDF660" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7306.168699999999, "isoelectric_point": 9.300805473327635 }, "id": "JPCNHPFPBM_201", "locus": "GCA000292505_00162" }, { "type": "cds", "sequence": "contig_1", "start": 87656, "stop": 87841, "strand": "+", "frame": 2, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002FAF0F9", "UniRef:UniRef90_UPI0002FAF0F9" ], "nt": "ATGACCAACTTGTTAAAACTACTGACAACTAAAAATGTGAAAGCAAAGTTGGGGAAGACAGCCCAATCTCAGGAAAATAGTGGGGGGGGTGAAATGATAAAAATGTTTTTAAATAACTACATTAATTCTCTTAAACTTACTAAATTATTTTTCCATTTCGAACTATTATTTGAAAAAAGTTATTAA", "aa": "MTNLLKLLTTKNVKAKLGKTAQSQENSGGGEMIKMFLNNYINSLKLTKLFFHFELLFEKSY", "aa_hexdigest": "0fb7cee5eabe72254b27e7df9e717341", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 87530, "stop": 87841, "observations": { "mutation": [ 87629 ], "stop": [ 87629 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0002FAF0F9", "query_cov": 1.0163934426229508, "subject_cov": 0.5688073394495413, "identity": 0.919, "score": 107.0, "evalue": 8.05e-29, "gene_start": 1, "gene_end": 61, "reference_start": 48, "reference_end": 109, "reference_sequence": "MDRREMWLASFLYYQPNKVKSGQYQTTNTYHRLIEPDKWQSNSDLTNMTSLLKLLTTKNIKQKLGKTAAQSQENNGGGEMIKMFLNNYINSLKLTKLFFHFELLFEKSY" }, "paralog": false, "identity": 0.875, "score": 180.0, "evalue": 2.13e-60, "description": "Internal stop codon at 87629. Nonsense mutation around 87629.", "psc": { "uniref90_id": "UniRef90_UPI0002FAF0F9", "product": "adhesin", "uniref50_id": "UniRef50_UPI0002FAF0F9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0002FAF0F9", "UniRef:UniRef50_UPI0002FAF0F9" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0002FAF0F9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002FAF0F9" ], "product": "adhesin" } }, "id": "JPCNHPFPBM_202", "locus": "GCA000292505_00163" }, { "type": "cds", "sequence": "contig_1", "start": 88319, "stop": 88627, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCCCTATTGCAGTTTTGCCTTTAGCAGGTATTTTTTTAGGAATCGGTGATGCCATTTCTTCCAATTCATCTGGCATTGTTGGTGTGAAATTTTTTGGTGAATTTATTAAACAAGGTGGTAATGTAGTTTTTGCTAACTTACCTATTTTGTTTGCAGTTGCAATTGCGATCACCTTTTCTCAAGATGCAGGGGTTGCTGGATTTTCTGCTTTTGTTTTTTGGGCCACAATGAACGCGTTTATGAGTTCATTAATTATTCCTGTTGATGCAAATAATACTGCTTCAGGTTATAACATCCTTTATTGA", "aa": "MIPIAVLPLAGIFLGIGDAISSNSSGIVGVKFFGEFIKQGGNVVFANLPILFAVAIAITFSQDAGVAGFSAFVFWATMNAFMSSLIIPVDANNTASGYNILY", "aa_hexdigest": "c509c2615c2ebfb2fb4f53bc0cc62f8f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10582.222899999993, "isoelectric_point": 4.225490379333496 }, "id": "JPCNHPFPBM_203", "locus": "GCA000292505_00164" }, { "type": "cds", "sequence": "contig_1", "start": 89074, "stop": 89331, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGAACCATCTAATAAACCGTTTTTAGCCAATTCATCAAAGTTACCTTTACGTGTAAGACCATCAACAAGTTCTTTTCAACTTAGAAAGTTTGTTGATTCTTTACTCATGTATTTTGAAAGTGCAGATGTACTATCTTGTCCTGTTGTAGTAGCTCCATTCATCATTCCACTTGTTCCATTAGCTAAAGCAGCAGGAATAACTCATTTTCCTGGAGTATTAAAATCTTCTCAACCTATCACTCTGGTTACATAA", "aa": "MLEPSNKPFLANSSKLPLRVRPSTSSFQLRKFVDSLLMYFESADVLSCPVVVAPFIIPLVPLAKAAGITHFPGVLKSSQPITLVT", "aa_hexdigest": "b5bfafef228a34c32e7120f9423cc423", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9225.878999999992, "isoelectric_point": 9.743316459655762 }, "id": "JPCNHPFPBM_204", "locus": "GCA000292505_00165" }, { "type": "cds", "sequence": "contig_1", "start": 89615, "stop": 89821, "strand": "+", "frame": 2, "gene": null, "product": "PTS transporter subunit EIIC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF73E8" ], "nt": "ATGCTAATTGGAATTCCAGCAGCTGGACTTGCAATGATCTTAGCTGCTCCTAAGGGTAGAAGAAAAGAAGCTAGTTCTATTATTGGTAGTGCTGCATTCACTAGTTTTCTAACAGGGATCACCGAACCTTTTGAATTTACCTTTCTTTTCTTAGCACCATGGTTATTCTATGGTATCCACGCTGTATTAGCTGCAGTAAGCTTTTGA", "aa": "MLIGIPAAGLAMILAAPKGRRKEASSIIGSAAFTSFLTGITEPFEFTFLFLAPWLFYGIHAVLAAVSF", "aa_hexdigest": "c0e42e1ce3e2c51319e018cf78de9d4e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021AF73E8", "query_cov": 0.8088235294117647, "subject_cov": 0.9166666666666666, "identity": 0.5820000000000001, "score": 63.5, "evalue": 4.71e-12, "valid": false, "product": "PTS transporter subunit EIIC", "uniref50_id": "UniRef50_UPI0021AF73E8" }, "pscc": { "uniref50_id": "UniRef50_UPI0021AF73E8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AF73E8" ], "product": "PTS transporter subunit EIIC" }, "genes": [], "id": "JPCNHPFPBM_205", "locus": "GCA000292505_00166" }, { "type": "cds", "sequence": "contig_1", "start": 89825, "stop": 89917, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTATTGAGTGCTAACGTTGGACAAACCTTCTCAGGTTCTTTCATTGACTTTATCTTGTATGGGGCTTTACCTGATGGTAGGGGTTGA", "aa": "MNLLSANVGQTFSGSFIDFILYGALPDGRG", "aa_hexdigest": "3cf8f7a3c4b9e071c17bda66713ca988", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3161.5407000000005, "isoelectric_point": 4.207074546813964 }, "id": "JPCNHPFPBM_206", "locus": "GCA000292505_00167" }, { "type": "cds", "sequence": "contig_1", "start": 89963, "stop": 90364, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00367.25" ], "nt": "TTGATTTATTTCCCTACCTTCTATTTCTTGACAATTCGCTTTAACTTAGCAACTCCTGGTAGAGGTGGTAAGTTAATTACTAAAAAAGAATATTTAGCAGCAAAAGCAGCTCAAAAAACTGATCAAACTACTAACACTAACTTTAATCAAACCCAAATTGAAGCTGGTATGTTACTAAGAGCTTATGGTGGAAGTGAAAACATTGCTGAATTAGGGGCTTGCATTACTAAATTAAGAGTAACAGTTAAAAACCCTGAACTTGTTAATGAAACTATTATTAAAGACTTGGGAGCAGCTGGGGTAATGCGTACCACTCCAACATTCTTTGTAGCAGTGTTTGGTACTCGAGCTGCTGTTTATAAATCAGCAATGCAAGATATTATCCAAGGCAAAGTAAATTGA", "aa": "MIYFPTFYFLTIRFNLATPGRGGKLITKKEYLAAKAAQKTDQTTNTNFNQTQIEAGMLLRAYGGSENIAELGACITKLRVTVKNPELVNETIIKDLGAAGVMRTTPTFFVAVFGTRAAVYKSAMQDIIQGKVN", "aa_hexdigest": "842393a5bb6a1c6635edb57534e7da7c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00367.25", "name": "PTS_EIIB", "length": 32, "aa_cov": 0.24060150375939848, "hmm_cov": 1.0, "evalue": 1.677932962494243e-13, "score": 44.932472229003906, "start": 58, "stop": 89 } ], "seq_stats": { "molecular_weight": 14589.788599999985, "isoelectric_point": 9.716110801696775 }, "id": "JPCNHPFPBM_207", "locus": "GCA000292505_00168" }, { "type": "cds", "sequence": "contig_1", "start": 90371, "stop": 90952, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00358.26" ], "nt": "TTGCAAAAAGTCTTAGATAAAAATGATAGTACTGTTGAAAAACCAGAAATAAAACCAACCCCAGTTTTAAAAGTTCAAGATGAAATTGTGATCCTCTCACCAGTTAATGGCACCTTAAAACCGCTCACCCAAGTTCCTGATGATACCTTCAAAAATCGTTTGGTAGGAGATGGAATTGCTATCTTACCTAGCGATGGGCACTTCAAAGCACCAGGTGATGTAGGTGTGAAAACTGAGCTTGCTTTCCCTACTGGTCATGCCTTTATCTTTGATGTTGATGGTGTGAAAGTAATGCTTCACATTGGGATTGATACAGTAAAAATTAATGCTGATAAAAAACCAGGGGAACAACTTGAAGTGTTTGATGTAAAAACAAAACAAGGAGAATACACTAAATTAAAGAGTGAAAGTGTTGTTGAAGTTGATTTAAAGAAACTTAAACGAAAGTATGATCCAATCACTCCTTTCATTGTGATGCAAGAATCACTTGATAACTTCAAGTTGGTACCAATTCGCCAACGTGGTGAAATTAAAGTCGGTCAACCTTTATTTAAATTAATTTATAAAGATAAGAAGAGTTAA", "aa": "MQKVLDKNDSTVEKPEIKPTPVLKVQDEIVILSPVNGTLKPLTQVPDDTFKNRLVGDGIAILPSDGHFKAPGDVGVKTELAFPTGHAFIFDVDGVKVMLHIGIDTVKINADKKPGEQLEVFDVKTKQGEYTKLKSESVVEVDLKKLKRKYDPITPFIVMQESLDNFKLVPIRQRGEIKVGQPLFKLIYKDKKS", "aa_hexdigest": "8ecbbbf44713947fe194fb2c4651b80b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00358.26", "name": "PTS_EIIA_1", "length": 134, "aa_cov": 0.6839378238341969, "hmm_cov": 0.917910447761194, "evalue": 2.60168684549907e-29, "score": 96.62474822998047, "start": 30, "stop": 161 } ], "seq_stats": { "molecular_weight": 21561.90710000001, "isoelectric_point": 8.857456398010253 }, "id": "JPCNHPFPBM_208", "locus": "GCA000292505_00169" }, { "type": "cds", "sequence": "contig_1", "start": 91063, "stop": 91155, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGATACAAATACTGAAAAACCTGAGTTAGTTTCCATTAATAAGTTAAGTGAGATGCGCACTAACATCGGGATGGTTAAACGTTATTGA", "aa": "MSDTNTEKPELVSINKLSEMRTNIGMVKRY", "aa_hexdigest": "8b974fbae25028224ad2fb3d36c077b5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3486.004500000001, "isoelectric_point": 8.189886283874515 }, "id": "JPCNHPFPBM_209", "locus": "GCA000292505_00170" }, { "type": "cds", "sequence": "contig_1", "start": 91165, "stop": 91380, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGATTCTTTATCGAACCTGAACGTAAGCATAATAACGATTTATTGAAGCTTGATCTACAGTACCAAGCGTTAAAAACTGCTTATAACTTCATTAAGGATGTTGTTAAAAATCACGGACAAATCCTTTTTGTTGGAACAAAGAATGATTATGTTAAAAAACTGGTAATTGATATTGCTAAAAGAGTTAATGTTGCATATATTACCCAGCGCTGA", "aa": "MGFFIEPERKHNNDLLKLDLQYQALKTAYNFIKDVVKNHGQILFVGTKNDYVKKLVIDIAKRVNVAYITQR", "aa_hexdigest": "0389f12c2bf01c4493ad346391329ca1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8338.6413, "isoelectric_point": 9.646162605285646 }, "id": "JPCNHPFPBM_210", "locus": "GCA000292505_00171" }, { "type": "cds", "sequence": "contig_1", "start": 91639, "stop": 91917, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCACTATGTAACACCAACACCAATCCAGAGCTAGTTGACTTTATTATTCCAGCTAATAACCACCAACCCCAAAGTACTTGTTTATTGATGAATTTACTAGCAGATGCGATAGCAGAAGCGAAGGGCTTTGAAACCTTGTATGCTTACAAACCAGATGAACAGATCCAAATTGAAATTCCTCCCAAACAAGAACGCCAAGTTATTAACCGTTCCAATACCAGAAACATCACTAACCAGCGCTTAAACATTAACCGCCAACAACAAGAAACTTTATAG", "aa": "MALCNTNTNPELVDFIIPANNHQPQSTCLLMNLLADAIAEAKGFETLYAYKPDEQIQIEIPPKQERQVINRSNTRNITNQRLNINRQQQETL", "aa_hexdigest": "c4ec16aa3b78c1dcdc7b7e018ba94fdf", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10563.82369999999, "isoelectric_point": 5.242680931091308 }, "id": "JPCNHPFPBM_211", "locus": "GCA000292505_00172" }, { "type": "cds", "sequence": "contig_1", "start": 92255, "stop": 92650, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACAAAGTCTTTCACAAGGGTTTTTAGGAATGGTGAATTAATTAGCATTAATGTTAGTGAAGTTGTTGTAGGAGATATTATTTTTGTTGATGCAGGAGATATTATCCCTGCTGATGGCAAATTACTACAGGTTAATAACTTACGTTGTTTGGAAAGCTTTTTAACTGGTGAATCAACTCCAGTTGATAAGACTATTGATAGCAATGAAAAAGCTACTATTCTTGAACAGACAAACTTAGTTTTTTCAGGGGCACAAGTAGTTTATGGTAGTGGCGTTTTTCAAGTGGAAGCAGTTGGGATTAAAACCCAAGTTGGAAAAATTGCTAAAACTGTTGATGATAGTGTAACTAAACTCTCACCCTTACAACAAAAACTAGAGAAGATAGGAAAGTGA", "aa": "MTKSFTRVFRNGELISINVSEVVVGDIIFVDAGDIIPADGKLLQVNNLRCLESFLTGESTPVDKTIDSNEKATILEQTNLVFSGAQVVYGSGVFQVEAVGIKTQVGKIAKTVDDSVTKLSPLQQKLEKIGK", "aa_hexdigest": "26ea0a1df61a9930a7013063e550e6f4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14114.037299999987, "isoelectric_point": 5.132242774963378 }, "id": "JPCNHPFPBM_212", "locus": "GCA000292505_00173" }, { "type": "cds", "sequence": "contig_1", "start": 92810, "stop": 93082, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCTTTGCATTAAGTGTACAGAAACTAACTAAGCAAAAAGCTATTATTAAGTATTTATCAGTAATTGAAACACTTGGATCAGTACAGATAATCTGTACTGATAAAACTGGTACTTTAACCCAAAACCAGATGAAAGTTGTCGATCACTTTTGTTTTAATTCAACAACCCAAACTGATCTAGCAAGAGCATTGTGTTTGTGTAACAACGCTTCTATTTCCAAAGATGAAAATAAAACAGGTGATCCTACTGAAATTGCTCTCTTGGAATGA", "aa": "MIFALSVQKLTKQKAIIKYLSVIETLGSVQIICTDKTGTLTQNQMKVVDHFCFNSTTQTDLARALCLCNNASISKDENKTGDPTEIALLE", "aa_hexdigest": "f9202203506084400e42aa7de09f6ce5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9909.393099999994, "isoelectric_point": 6.545771598815918 }, "id": "JPCNHPFPBM_213", "locus": "GCA000292505_00174" }, { "type": "cds", "sequence": "contig_1", "start": 93158, "stop": 93652, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00702.32" ], "nt": "ATGACAGTTGTCGTTCAAAAAGACAACCGCTTCATTGTGATTGTTAAAGGTGCTCCTGATGTGTTATTACCATTATGTAATAACGTTCAAAATGAAGTAAAAAACATTGAAAACTTACTTGATCAAAGTGCTGGTCAAGGCTTGCGTACCTTAGCAGTTGCTTTAAAGGTTTTATATAAGTTTGATCAAAACGATCAGAAGCAAATTGATGAACTTGAAAACAACCTTGAATTCCTTGGGTTTGTTAGTTTACAAGACCCACCAAGAAAAGAAAGTAAGGAAGCGATTTTAGCATGCAAAAAAGCTAATATAACCCCAATAATGATTACAGGGGATCATCTTAAAACTGCAACTGCAATTGCTAAAGAGTTAGGCATTTTAATTTTAGATAATCAAGCAGTTTTAGGTAGTGAACTAGATGAAAAGAAGATCTTGGATTACAGGGTATTTGCTAGAGTAACTCCCCAACAAAAATTAGCCATTGTTAGTGCTTGA", "aa": "MTVVVQKDNRFIVIVKGAPDVLLPLCNNVQNEVKNIENLLDQSAGQGLRTLAVALKVLYKFDQNDQKQIDELENNLEFLGFVSLQDPPRKESKEAILACKKANITPIMITGDHLKTATAIAKELGILILDNQAVLGSELDEKKILDYRVFARVTPQQKLAIVSA", "aa_hexdigest": "5695f88841f8f626cb830550e2e42531", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00702.32", "name": "Hydrolase", "length": 122, "aa_cov": 0.7134146341463414, "hmm_cov": 0.8934426229508197, "evalue": 4.726714535364667e-10, "score": 35.25105667114258, "start": 46, "stop": 162 } ], "seq_stats": { "molecular_weight": 18178.947699999986, "isoelectric_point": 5.883824729919433 }, "id": "JPCNHPFPBM_214", "locus": "GCA000292505_00175" }, { "type": "cds", "sequence": "contig_1", "start": 93692, "stop": 94132, "strand": "+", "frame": 2, "gene": null, "product": "HAD-IC family P-type ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB434DA", "UniRef:UniRef90_UPI001FB434DA" ], "nt": "GTGAATGACGCACCTGCATTAATCAAGAGTGATGTAGGGTGTTGTATGGGGATTACCGGGGTTGATATTGCAAAAGATGCTAGTGATCTGATTATTAGTGATGATAATTTCGCTACTATAGTAAATGGTATTGAACAGGGTAGAAAAACCTTTTTAACTTGTAAACGAGTTTTATTAAACCTGTTTTTAACTTCAATTGCAGGAACAGTTGTAGTTTTATTAGGACTATTCATCTTAGGACAAGTTTTTAAAACTAATTTATTACAACAAGGTCATGACTTTCAGGTGTTTAGTCCTACCCAACTGCTAATTATTAACTTGTTTGTTCATGGTTTTCCTGCTGTTGCATTAGCAGTACAACCTGTTAAAGAAAAATTGATGGTAGGTAGTTTTTCTACTAAAAATCTGTTTTACAACCGCCAGGGATTTGATTTAATCTGA", "aa": "MNDAPALIKSDVGCCMGITGVDIAKDASDLIISDDNFATIVNGIEQGRKTFLTCKRVLLNLFLTSIAGTVVVLLGLFILGQVFKTNLLQQGHDFQVFSPTQLLIINLFVHGFPAVALAVQPVKEKLMVGSFSTKNLFYNRQGFDLI", "aa_hexdigest": "1ac44b39b3fe3d0be902c730bf27ec19", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 93527, "stop": 94354, "observations": { "mutation": [ 93650, 94130 ], "stop": [ 93650, 94130 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001FB434DA", "query_cov": 1.0, "subject_cov": 0.5270758122743683, "identity": 0.8290000000000001, "score": 241.0, "evalue": 2.93e-78, "gene_start": 1, "gene_end": 146, "reference_start": 56, "reference_end": 201, "reference_sequence": "MGILTDERQAILGVDLDPAKIMEYRVFARVTPQQKLEIVNAWKQAGYTVAVTGDGVNDAPALVTSDVGCCMGQTGVDIAKDAADVIISDDNFATIVNGIEQGRKTFLTCKRVLFNLFLTSIAGTIVVLLGLFVLGEVFREQLSKANHNFQVFTPTQLLIINLFVHGFPAVALAIQPVQEKLMLKPFSTKNLFYNRGGFDLIWQSLLLSFLTLLFYSLGMVYAINDPELGKSGDLINRAGATCGFMVLGGSAALNSLNLMVDRPLVATNPKHYGIVXX" }, "paralog": false, "identity": 0.7709090909090909, "score": 424.0, "evalue": 1.55e-152, "description": "Internal stop codon at 93650, 94130. Nonsense mutation around 93650, 94130.", "psc": { "uniref90_id": "UniRef90_UPI001FB434DA", "product": "HAD-IC family P-type ATPase", "uniref50_id": "UniRef50_UPI001FB434DA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001FB434DA", "UniRef:UniRef50_UPI001FB434DA" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001FB434DA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB434DA" ], "product": "HAD-IC family P-type ATPase" } }, "id": "JPCNHPFPBM_215", "locus": "GCA000292505_00176" }, { "type": "cds", "sequence": "contig_1", "start": 94168, "stop": 94395, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCAATAAAAATACCAGTATAGAAGCAAGTGAACCTATTCAAACTAACTTAAAAAACCAAGGGTTTGTCATAAGCAATGGTTTATCTACCATTAGGTTTAATGAGTTTAAAGCAGCACTAGCACCCAAAATAAAAAAACCGCACGTTGATCCAGCACGATTAATTAGATCCCCGCTAGTTTGTAAATCACGGTTATTAATTGCATATATAATTCCTAAGCTATAG", "aa": "MINKNTSIEASEPIQTNLKNQGFVISNGLSTIRFNEFKAALAPKIKKPHVDPARLIRSPLVCKSRLLIAYIIPKL", "aa_hexdigest": "bca55a16b16ca7405203fd8d8eab7927", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8370.879199999996, "isoelectric_point": 10.294843482971189 }, "id": "JPCNHPFPBM_216", "locus": "GCA000292505_00177" }, { "type": "cds", "sequence": "contig_1", "start": 94533, "stop": 95780, "strand": "+", "frame": 3, "gene": "secA", "product": "preprotein translocase subunit SecA", "db_xrefs": [ "COG:COG0653", "COG:U", "EC:7.4.2.8", "GO:0005524", "GO:0005737", "GO:0005886", "GO:0006605", "GO:0008564", "GO:0009898", "GO:0015450", "GO:0016887", "GO:0017038", "GO:0031522", "GO:0043952", "GO:0065002", "SO:0001217", "UniRef:UniRef50_P47318", "UniRef:UniRef90_P47318" ], "nt": "ATGGCAATATTTAACTTCCTTAAGTTAATTTCACCCAAAAACAGAATTCTCAGTAAGGCAAATAGGATTGCCAGTGAGGTTGAGAGTTATAAAAACTACTACCGTAACTTAACTGATCAACAGTTATTTGAAGAGTCAAATAAACTAGTTGATCTTGTCACTAAGCAAAATTACACCATTCTAGATGTTTGTGTTGCTGCACTTGCTTTAATTAGAGAAGTGGTTTACCGTGAGACTGGTGAATTTGCATATAGGGTGCAGATCATAGGAGCTTTTATTGTTTTAAGTGGTGATTTTGCTGAGATGATGACTGGTGAAGGTAAGACCTTAACCATTGTTTTAGCAGCATACGTTTCTGCACTTGAAAAGCGTGGTGTGCATGTTGTTACTGTCAATGAATATCTAGCTCAAAGGGATGCTAATAATGCAATGAAGATCTTAAAACGGGTTGGGATGAGTGTTGGTTGTAACTTTGCTAACCTCTCCCCTCAGCTAAAACAAGCTGCATTTAATTGCGATGTTACCTACACCACTAACAGTGAACTGGGGTTTGATTATCTTAGAGATAACATGGTCCACAGTTATCAAGATAAGAAGATCAGAGAGTTGCACTTTGCAATAGTTGATGAAGGTGATTCAGTTTTAATTGATGAGGCGCGAACGCCTTTAATTATTTCAGGTCCTAGTAAAAATGAGTTTGGGTTATATGTTGCAGTTGATCGATTTGTTAAATCATTAACTGAACAGGAGTTTAAGATTGACCCTGAATCACGTGCTGCTTCTTTAACTGAACTTGGGATTAAAAAAGCAGAGCAAACATTTAAAAAAGAAAACCTTTTTGCTTTGGAAAACAGTGATCTTTTTCACAAGATCATGAATGGTTTGACTGCTGTGAAAGTTTTTGAACAGGGCAAAGAGTACATTGTTCGTGATGGCAAGGTTTTAATTGTTGATCACTTTACAGGTAGGATATTGGAAGGGAGAAGTTACAGTAATGGCTTACAACAAGCTGTACAAGCCAAAGAATATGTTGAGATAGAACCTGAAAATGTGATAGTAGCTACCATTACCTACCAATCCTTCTTTAGGCTATACAACCGCTTAGCAGCAGTATCAGGTACTGCTTTAACTGAATCAGAGGAGTTTCTCAAGATTTATAACATGGTTGTAGTACCAGTACCAACTAACCGTCCTAACATCAGAAAAGACCGTTCTGATAGTGTATTTGGTACCCCACAAATTAAGTGA", "aa": "MAIFNFLKLISPKNRILSKANRIASEVESYKNYYRNLTDQQLFEESNKLVDLVTKQNYTILDVCVAALALIREVVYRETGEFAYRVQIIGAFIVLSGDFAEMMTGEGKTLTIVLAAYVSALEKRGVHVVTVNEYLAQRDANNAMKILKRVGMSVGCNFANLSPQLKQAAFNCDVTYTTNSELGFDYLRDNMVHSYQDKKIRELHFAIVDEGDSVLIDEARTPLIISGPSKNEFGLYVAVDRFVKSLTEQEFKIDPESRAASLTELGIKKAEQTFKKENLFALENSDLFHKIMNGLTAVKVFEQGKEYIVRDGKVLIVDHFTGRILEGRSYSNGLQQAVQAKEYVEIEPENVIVATITYQSFFRLYNRLAAVSGTALTESEEFLKIYNMVVVPVPTNRPNIRKDRSDSVFGTPQIK", "aa_hexdigest": "e958cebffacd0c1992c5bdc837f2fc3d", "start_type": "ATG", "rbs_motif": null, "genes": [ "secA" ], "truncated": "3-prime", "pseudogene": { "start": 94533, "stop": 96083, "observations": { "mutation": [ 95778 ], "stop": [ 95778 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47318", "query_cov": 1.0, "subject_cov": 0.5142503097893433, "identity": 1.0, "score": 801.0, "evalue": 1.93e-286, "gene_start": 1, "gene_end": 415, "reference_start": 1, "reference_end": 415, "reference_sequence": "MAIFNFLKLISPKNRILSKANRIASEVESYKNYYRNLTDQQLFEESNKLVDLVTKQNYTILDVCVAALALIREVVYRETGEFAYRVQIIGAFIVLSGDFAEMMTGEGKTLTIVLAAYVSALEKRGVHVVTVNEYLAQRDANNAMKILKRVGMSVGCNFANLSPQLKQAAFNCDVTYTTNSELGFDYLRDNMVHSYQDKKIRELHFAIVDEGDSVLIDEARTPLIISGPSKNEFGLYVAVDRFVKSLTEQEFKIDPESRAASLTELGIKKAEQTFKKENLFALENSDLFHKIMNGLTAVKVFEQGKEYIVRDGKVLIVDHFTGRILEGRSYSNGLQQAVQAKEYVEIEPENVIVATITYQSFFRLYNRLAAVSGTALTESEEFLKIYNMVVVPVPTNRPNIRKDRSDSVFGTPQIKWMAVVKEIKKIHETSRPILIGTANIDDSELLHNLLLEANIPHEVLNAKNHSREAEIVAKAGQKNAVTISTNMAGRGTDIRLGEGVAEMGGLYVLGTERNESRRIDNQLRGRAARQGDKGETKFFISLGDSLFKRFAHDKIERAISKLGNETFDSAFFSKMLSRTQKRVEAINFDTRKNLIDYDHVLASQRELIYKQRDKFLLANDLSEMIDKMLEKFVQQFCDQYRNQKNQNLINHIALAEALNLEMNMQNTINPKVFENMTFDVAVDKTRNLVAKKISDKVNVLTKPIALNRFRDIIITSMDKHWTEHLDSVFKLREGVVLRSMEHTSPLNVYIKETDILFKTMLQKIAQDVIVQIANLTTPDEFDHSLMQANALKKLAAIKADEKSNQEQ" }, "paralog": false, "identity": 0.998062015503876, "score": 996.0, "evalue": 0.0, "description": "Internal stop codon at 95778. Nonsense mutation around 95778.", "psc": { "uniref90_id": "UniRef90_P47318", "gene": "secA", "product": "preprotein translocase subunit SecA", "ec_ids": [ "7.4.2.8" ], "uniref50_id": "UniRef50_P47318", "cog_id": "COG0653", "cog_category": "U", "go_ids": [ "GO:0005524", "GO:0005737", "GO:0005886", "GO:0006605", "GO:0008564", "GO:0009898", "GO:0015450", "GO:0016887", "GO:0017038", "GO:0031522", "GO:0043952", "GO:0065002" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47318", "EC:7.4.2.8", "UniRef:UniRef50_P47318", "COG:COG0653", "COG:U", "GO:0005524", "GO:0005737", "GO:0005886", "GO:0006605", "GO:0008564", "GO:0009898", "GO:0015450", "GO:0016887", "GO:0017038", "GO:0031522", "GO:0043952", "GO:0065002" ] }, "pscc": { "uniref50_id": "UniRef50_P47318", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47318" ], "product": "Protein translocase subunit SecA" } }, "id": "JPCNHPFPBM_217", "locus": "GCA000292505_00178" }, { "type": "cds", "sequence": "contig_1", "start": 95781, "stop": 96695, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF21090.3" ], "nt": "ATGGCAGTTGTTAAAGAGATAAAAAAGATCCATGAAACTTCTCGACCTATTCTGATTGGAACTGCTAATATAGATGATTCTGAACTCTTACATAATCTGTTACTAGAAGCTAATATTCCCCATGAGGTTTTAAATGCTAAAAACCATTCAAGAGAAGCGGAGATAGTAGCTAAAGCAGGACAGAAGAATGCAGTGACTATTTCAACTAACATGGCTGGAAGGGGAACTGATATCCGTTTAGGTGAAGGGGTTGCTGAAATGGGTGGTCTTTATGTATTGGGAACTGAAAGAAATGAGTCAAGAAGGATTGATAACCAACTAAGAGGGAGAGCTGCTAGACAAGGTGATAAAGGGGAAACTAAGTTCTTTATCTCACTAGGTGATTCATTGTTTAAACGTTTTGCTCATGACAAGATTGAAAGAGCGATTAGCAAATTAGGTAATGAAACATTTGACAGTGCCTTCTTTTCCAAAATGTTAAGTAGAACCCAAAAACGGGTGGAAGCAATTAACTTTGACACTAGAAAAAACCTGATTGATTATGACCATGTTCTTGCAAGTCAAAGGGAATTGATTTACAAACAACGTGATAAGTTTTTATTAGCAAACGATTTAAGTGAAATGATCGACAAAATGCTAGAAAAGTTTGTACAACAGTTTTGTGATCAATATAGAAACCAAAAGAACCAAAACTTAATTAATCACATTGCACTAGCAGAAGCTTTAAATCTTGAGATGAACATGCAAAACACCATTAATCCAAAGGTGTTTGAAAACATGACTTTTGATGTTGCTGTTGATAAAACCCGTAACTTAGTAGCTAAAAAGATTAGTGATAAAGTTAATGTTTTGACCAAACCAATTGCTTTAAACAGGTTTCGTGACATTATCATAACTTCGATGGATAAACATTGA", "aa": "MAVVKEIKKIHETSRPILIGTANIDDSELLHNLLLEANIPHEVLNAKNHSREAEIVAKAGQKNAVTISTNMAGRGTDIRLGEGVAEMGGLYVLGTERNESRRIDNQLRGRAARQGDKGETKFFISLGDSLFKRFAHDKIERAISKLGNETFDSAFFSKMLSRTQKRVEAINFDTRKNLIDYDHVLASQRELIYKQRDKFLLANDLSEMIDKMLEKFVQQFCDQYRNQKNQNLINHIALAEALNLEMNMQNTINPKVFENMTFDVAVDKTRNLVAKKISDKVNVLTKPIALNRFRDIIITSMDKH", "aa_hexdigest": "ad9c5f218455bf6bddd4cef68ae756cd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF21090.3", "name": "P-loop_SecA", "length": 81, "aa_cov": 0.26644736842105265, "hmm_cov": 1.0, "evalue": 9.714776872157141e-64, "score": 209.8253936767578, "start": 2, "stop": 82 } ], "seq_stats": { "molecular_weight": 34671.368500000026, "isoelectric_point": 9.228987693786618 }, "id": "JPCNHPFPBM_218", "locus": "GCA000292505_00179" }, { "type": "cds", "sequence": "contig_1", "start": 96750, "stop": 96956, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACATACAAGTCCTTTAAATGTTTACATTAAAGAAACAGATATCCTTTTTAAAACAATGTTGCAAAAGATTGCTCAAGATGTCATTGTGCAAATTGCTAACCTCACAACTCCAGATGAATTTGATCATAGCTTAATGCAAGCTAATGCTTTAAAGAAACTAGCAGCAATTAAAGCAGATGAAAAATCAAACCAAGAGCAATAG", "aa": "MEHTSPLNVYIKETDILFKTMLQKIAQDVIVQIANLTTPDEFDHSLMQANALKKLAAIKADEKSNQEQ", "aa_hexdigest": "566e63e54ba453d49654dff1cdacf762", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7713.770300000001, "isoelectric_point": 5.134175300598144 }, "id": "JPCNHPFPBM_219", "locus": "GCA000292505_00180" }, { "type": "cds", "sequence": "contig_1", "start": 96931, "stop": 98901, "strand": "+", "frame": 1, "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "db_xrefs": [ "COG:COG0556", "COG:L", "GO:0003677", "GO:0005524", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432", "GO:0016887", "KEGG:K03702", "RefSeq:WP_014894561.1", "SO:0001217", "UniParc:UPI00027B33FF", "UniRef:UniRef100_UPI00027B33FF", "UniRef:UniRef50_P47319", "UniRef:UniRef90_P47319" ], "nt": "ATGAAAAATCAAACCAAGAGCAATAGTTTATTTCAACTTACCACTAACTATATACCTACTGGTGATCAACCTGAAGCAATTAAGAAACTATCAGAATTTAAAACTAAGCAGCAGGTTTTATTGGGGGCTACAGGCACAGGTAAAACCTTTACAATTGCTAATGTAATTCAAAACAGCCAACTCCCAACAGTTGTTATTGCTCATAACAAAACCCTAGCAGGTCAACTCTTCAATGAATTAAAACAACTGTTTCCTAAAAATGCAGTTGAATATTTTATCTCTTACTTTGATTTTTATCAACCTGAAGCTTACTTACCCAGTAAAGGGATCTACATTGAAAAAAGTGCTACAGTCAATGAAGCAATTAAACGCTTAAGAGTCTCAACACTGCATTCACTTTCAACAAGAAAAGATGTTATTGTAGTGGGTTCAGTTGCTAGTATTTATCCCACCTCATCTCCCAGTGATTTTGTTAAGTATTGCTTGTGGTTTGTGGTTGGCAAAGATTATGATTTGAAAACCATTAAAGATAGGTTAGTTAGTCTTAACTATGTTGTTAATAAACAACAATTAACCCCAGGAAAATTTCGCTTCCAGGGTGATGTTTTGGAAGTATTTCCTGGTTACAGTGATGCTTTTGTGATCAGAATCTCTTTTTTTGATACTAAAGTAGAACAAATTTGTCAAATTGACCCACTAACAAATAAGATTTTAAACCAACTCTTTGAGATTAAGATAGGTCCTGCTGATGAATATGTTGTAAACCAATCTGATCTTGATATAGCAATTAAAAATATTAAACAAGAACTTCAGGAACGAGTTAATTATTTCAATAAGCAAAATCTTGTTGAAAGAGCACAACGTTTAGCCACCATTACTAACCATGATCTCAATGATTTGAAGGCTTGGGGATTTTGTAGTGGAGTTGAAAACTATGCTAGACACTTAGAGTTGAGGATGGCTAACTCAACCCCTTACAGTATCTTTGATTATTTTAAGGGGGATTGGTTATTGGTTATTGATGAATCACACCAAACTTTACCGCAACTTAATGGGATGTATAACACTGATCTTTCAAGAAAACAAAGCTTAATTGATTATGGTTTTCGACTCCCCTCTGCACTTGATAACAGACCGCTCTCATTTGCTGAATTACAACAAAAAATGCAAAAAGTTATTTATGTTTCAGCAACTCCAAGAGATAAAGAGATTAGTTTAAGTCAGAATAATGTCATTGAACAGTTAGTTAGACCAACCTACTTGGTTGATCCTATTATCGTTGTTAAACCAAAAGATAACCAGGTGGAGGATCTCATTGAAGAGATTATCAACCAACGCCAAAACAACACAAGAACATTTGTTACTGTTTTAACTATTAAGATGGCTGAAAACCTCACTGAATACTTAAAGGAACGCAAAATTAAAGTTGCCTATATCCATAAGGACATTAAAGCATTGGAACGTTTATTGTTAATTAATGACCTGAGAAGAGGTGAATATGAGTGTTTAGTTGGGATTAACCTTTTAAGAGAAGGGTTAGATGTCCCTGAAGTTGCTTTAGTTTGTATCTTTGATGCAGATATCCCAGGACTACCTAGGGATGAGAGAAGTTTAATCCAGATTATTGGACGTGCTGCTAGAAATGAACATGGTCGAGTTGTTATGTATGCTAACCATGTTACTGAACAGATGCAAAAAGCCATTGATGAAACCAAAAGAAGAAGAACTGTTCAAATGGAATATAACAAGCTACATAATAAGATACCAAAAACAGTTGTTAAACCCCTTAGCTTTGTTCAACCAATCAAATTAAAAGCTAAGAGTAATGCAGAAAAAAATGCTGCATTAATCAAACAATTAACCAAAGAAATGAAGAAAGCAGCAGCTAATCAAAATTATGAACTTGCCATTGAGATTAGAGATTCCATATTTGAATTGGAAAAAGAAATTGATAGTAAAATTAAAGTATAG", "aa": "MKNQTKSNSLFQLTTNYIPTGDQPEAIKKLSEFKTKQQVLLGATGTGKTFTIANVIQNSQLPTVVIAHNKTLAGQLFNELKQLFPKNAVEYFISYFDFYQPEAYLPSKGIYIEKSATVNEAIKRLRVSTLHSLSTRKDVIVVGSVASIYPTSSPSDFVKYCLWFVVGKDYDLKTIKDRLVSLNYVVNKQQLTPGKFRFQGDVLEVFPGYSDAFVIRISFFDTKVEQICQIDPLTNKILNQLFEIKIGPADEYVVNQSDLDIAIKNIKQELQERVNYFNKQNLVERAQRLATITNHDLNDLKAWGFCSGVENYARHLELRMANSTPYSIFDYFKGDWLLVIDESHQTLPQLNGMYNTDLSRKQSLIDYGFRLPSALDNRPLSFAELQQKMQKVIYVSATPRDKEISLSQNNVIEQLVRPTYLVDPIIVVKPKDNQVEDLIEEIINQRQNNTRTFVTVLTIKMAENLTEYLKERKIKVAYIHKDIKALERLLLINDLRRGEYECLVGINLLREGLDVPEVALVCIFDADIPGLPRDERSLIQIIGRAARNEHGRVVMYANHVTEQMQKAIDETKRRRTVQMEYNKLHNKIPKTVVKPLSFVQPIKLKAKSNAEKNAALIKQLTKEMKKAAANQNYELAIEIRDSIFELEKEIDSKIKV", "aa_hexdigest": "ea022dbe062874dfbd600269629d2909", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B33FF", "ncbi_nrp_id": "WP_014894561.1", "uniref100_id": "UniRef100_UPI00027B33FF", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B33FF", "RefSeq:WP_014894561.1", "UniRef:UniRef100_UPI00027B33FF" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B33FF", "uniref90_id": "UniRef90_P47319" }, "psc": { "uniref90_id": "UniRef90_P47319", "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "uniref50_id": "UniRef50_P47319", "cog_id": "COG0556", "cog_category": "L", "kegg_orthology_id": "K03702", "go_ids": [ "GO:0003677", "GO:0005524", "GO:0005737", "GO:0006289", "GO:0009380", "GO:0009381", "GO:0009432", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47319", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47319" ], "product": "UvrABC system protein B" }, "genes": [ "uvrB" ], "id": "JPCNHPFPBM_220", "locus": "GCA000292505_00181" }, { "type": "cds", "sequence": "contig_1", "start": 98904, "stop": 99110, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MG074", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47320", "UniRef:UniRef90_P47320" ], "nt": "ATGCGACAGTTTATTAAGCTTAGTCTCTTAGTCTTTGTCTTGCTTTTCTTAAGTGAACTTATCTGTAGGTTCTCTTTACGCTTAGTAAATTCTATTAAAGCGAGATATAAATCTTCAGTATTTTCCTACACTGCTTGTTTGCTATTTTTAAAGAGTTTTCAAAACTTTTCTAATGCTTTTCAGAAGTTAGCTAACTGGGTATTTTGA", "aa": "MRQFIKLSLLVFVLLFLSELICRFSLRLVNSIKARYKSSVFSYTACLLFLKSFQNFSNAFQKLANWVF", "aa_hexdigest": "439da34ab80564d5557d144c90f8276c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 98904, "stop": 99317, "observations": { "mutation": [ 99108, 99294 ], "stop": [ 99108, 99294 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47320", "query_cov": 1.0, "subject_cov": 0.49635036496350365, "identity": 1.0, "score": 126.0, "evalue": 6.01e-36, "gene_start": 1, "gene_end": 68, "reference_start": 1, "reference_end": 68, "reference_sequence": "MRQFIKLSLLVFVLLFLSELICRFSLRLVNSIKARYKSSVFSYTACLLFLKSFQNFSNAFQKLANWVFWFENDVNELLSIFYFNFDQKSEKVDYNFFNGYKVTAQKVVEKEQLLTCKLSDYYRLFRDKTFWFELINN" }, "paralog": false, "identity": 0.9854014598540146, "score": 267.0, "evalue": 3.44e-94, "description": "Internal stop codon at 99108, 99294. Nonsense mutation around 99108, 99294.", "psc": { "uniref90_id": "UniRef90_P47320", "product": "Uncharacterized protein MG074", "uniref50_id": "UniRef50_P47320", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47320", "UniRef:UniRef50_P47320" ] }, "pscc": { "uniref50_id": "UniRef50_P47320", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47320" ], "product": "Uncharacterized protein MG074" } }, "id": "JPCNHPFPBM_221", "locus": "GCA000292505_00182" }, { "type": "cds", "sequence": "contig_1", "start": 99381, "stop": 101777, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MG075", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47321", "UniRef:UniRef90_P47321" ], "nt": "ATGAAATTAAGTACAATAACTACTATCTGCTTGTCAATATCTGGTGCTTTTGGTACTACTGCAATTGCTTTACCAACTACAGTAGCATTGTTAAAAAACCACCAACAGCAAAACACTGAAAAACAACAAAACCCGATTAAAGATATCCGCTTTGGTTTAAACAATGTACAGGTTCCAAATACCATTCCGTTACACCAAACTGTGGTTGAAGTGATCAACAACAAAGAAATTGTTGATTACAAAGATGCACCCCAAAAACTCTTTTTAGCTAAAAGTGCGTTAAATAATAAACTCCAAGTTGAGTTTGATAAGTTCTTGCTAAGAACAGGAGTAATTAATGCTTTAAATGCAGATTTGAAAGAATGGATAGACCAAACATTATTTATCCCCAATCAAAGTTTTTTTGATCTTAGTGCTAATAAACTCAATTTAACTTTGTCAAATCAAGGCGAAGTGAGTTTAGATCTTGATTTTACTTTTACTAACTTTAGTGATAAAAATCAACCATTAAAACTCCCCTTTGACGGTAATGTGGTGGTTAATGCTAACGAATCATACACCTATTCAGTTAAAGCAACACTACAGAAACTAAAAGTATTGACTTTTTCAAGAGCAGATCATTATGTAGGGATTAGTTATGCAATACCAAAAGTGAGTTTAAATGGTAAAACTCAAAATGACTTTAGTTTTAACCCTTTTAAAAGTAATATTAACTTTACTTTTAAAAATGTCTACAATGCTTTAGATCCGTTTGAAGCACAAAAATACCTGGTTGGCCAAGGCAAGTTTTTAAACCAAAAAGTTAATGCTGATGATGTTAAAAATGACATTAATAACCACATTGAAACCCAGTTTAATGTTGCTAAAATCACAGCAACTCTTTTAGGAAAAGCTTTTAAGCAGTTTGGTGAACATAAAAATGGTCAACCACTTTCACTTTTAAAAGTACTAAGTGGATTAAACAATGAGTTTAAACAACTCTTTAATTATGTCAGACCTGGTTTAGGTGATTTTGTTAGTGATTTAATCCAAAGCTCCAGTCAATCAAGTAATAAAAAAACTGTTTACCAGCTATTATTTGAAAACAAGACAACAATTATCCACTTGTTGCAAGATCTTAATATTAGTGAACTGAACTCTGTTTTACCAGTTGTTGATATTTTGTTTGAAGGGATTAACAGTGCAGAATCACTATACCAAAGGATCCAATCATTTAAGGATTTAATTGTTCCTGCTTTAAAAGCTGATAAGCAACTTAAGAGCTTAGAAGCGATTATTTTAGCAGTTTTAGACAATCCTAATACCTATGTTTTTGATCTTGTTTACCAAAACAAATCGATTCTGTTTAATTTGTTAAGTGATTTTCTTAAAAACACTGCAAACACTTTACCATTTCTTCAAGAACAGTTTGATATTGTTAACCACTTATTTGCCAATGAAGCGATCTTTGATCTGTTTTCCAATGCTGACTTTGTTGATAAGATTGCTGATCTTTTTTTAGCAAAACAAAAGGTACAAGAAGTTAATAATGATGGAACTAAATCAACAAAAATTGTTGATTCGATTTTAGTTGCTACCCTTAAGGGTTTAGTTGGTAATCAGCTCAGTTCAATCACTGAACTGTTAAACATCTATATCTTTGAAAATGAATTTTTAAACAGAAATGATAGTAATAGTTCAGTTAAAAAACAACAAACTGATAGTCTAAAAAATCTGTTTAGCGTAATTGGTGATATTTTAAGTGAGACCAATGTTAACAAGATCACTTTACACGCAGTTAAAAATAATGAACTTCTTTCGTTAGTTGAAACAGCTTCAACACTAAAAATTAAACATCTTAATGTTCAATACAAAGTTTTAGTTGATAAGTTTGAATTGAAAAACAGTTTCATTAAGGCGCTATTAAACTTTTTCCCAGATACTAAAGATATCACCCCAACTATTAAAAAGGTGCTGTTTGAAAGTGAAAACTATAAAACACTCCGTAGGAAGTATGAAAATGAAGGGTTCCCAGGTTATCACTGGGCTAAGTTCATTGTCCCAGGTACATTCAATTCTGCTGAAAACACTTTCTATTCTGCTATTGATAAAACTAAATCAATTCGTGATCTGTTTGCTGACATGTTATTTGGTAAGAGTTTAGAGAGTGTTAATGACAATGATAGCTTTATCAAAATTAATGGTTCATTTACATTAAAGTACCACGGTGATAACTTAAATTTACTCCCTAACTACCACTCATTAATCACTAAGAATGTTGGTTATCAAATAGTTAATGTTAATTTTCATATTGATGCAAGATTGCTAACTGCAGAACTACAAAACACGGTATTTTCAAATCCAAAACCAGTTATTAAATCACCTGTTGAACTCTCCAAATCCTTATTTGAAGTTTGA", "aa": "MKLSTITTICLSISGAFGTTAIALPTTVALLKNHQQQNTEKQQNPIKDIRFGLNNVQVPNTIPLHQTVVEVINNKEIVDYKDAPQKLFLAKSALNNKLQVEFDKFLLRTGVINALNADLKEWIDQTLFIPNQSFFDLSANKLNLTLSNQGEVSLDLDFTFTNFSDKNQPLKLPFDGNVVVNANESYTYSVKATLQKLKVLTFSRADHYVGISYAIPKVSLNGKTQNDFSFNPFKSNINFTFKNVYNALDPFEAQKYLVGQGKFLNQKVNADDVKNDINNHIETQFNVAKITATLLGKAFKQFGEHKNGQPLSLLKVLSGLNNEFKQLFNYVRPGLGDFVSDLIQSSSQSSNKKTVYQLLFENKTTIIHLLQDLNISELNSVLPVVDILFEGINSAESLYQRIQSFKDLIVPALKADKQLKSLEAIILAVLDNPNTYVFDLVYQNKSILFNLLSDFLKNTANTLPFLQEQFDIVNHLFANEAIFDLFSNADFVDKIADLFLAKQKVQEVNNDGTKSTKIVDSILVATLKGLVGNQLSSITELLNIYIFENEFLNRNDSNSSVKKQQTDSLKNLFSVIGDILSETNVNKITLHAVKNNELLSLVETASTLKIKHLNVQYKVLVDKFELKNSFIKALLNFFPDTKDITPTIKKVLFESENYKTLRRKYENEGFPGYHWAKFIVPGTFNSAENTFYSAIDKTKSIRDLFADMLFGKSLESVNDNDSFIKINGSFTLKYHGDNLNLLPNYHSLITKNVGYQIVNVNFHIDARLLTAELQNTVFSNPKPVIKSPVELSKSLFEV", "aa_hexdigest": "e467e9b96d9eb41e9c1b358d1eb8cf73", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 99381, "stop": 102080, "observations": { "mutation": [ 101775 ], "stop": [ 101775 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47321", "query_cov": 1.0, "subject_cov": 0.779296875, "identity": 0.977, "score": 1508.0, "evalue": 0.0, "gene_start": 1, "gene_end": 798, "reference_start": 1, "reference_end": 798, "reference_sequence": "MKLSTITTICLSISGAFGTTAIALPTTVALLKNHQQQNTEKQQNPIKDIRFGLNNVQVPNTIPLHQTVVEVTNNKAIVDYKDAPQKFFLAKSALNNKLQVEFDKFLLRTGVINALNADLKEWIDQTLFIPNQSFFDLSANKLNLTLSNQSEVSLDLEFIFTNFSDKNQPLKLPFDGSVVVNANESYTYSVKATLQKLKVLTYSRADHSVGISYAIPTVSLNGKTQNDFSFNPFKSNINFAFKNVYNALNPFEAQQYLVGQGKFLNQKVNADDVKNDINNHIETQFNVAKITATLLGKAFKQFGEHKNGQPLSLLKVLSGLNNEFKQLFNYVRPGLGDFVSDLIQSSSQSSNKKTVYQLLFENKTTIIHLLQDLNISELNSVLPVVDILFEGINSAESLYQRIQSFKDLIVPALKADKQLKSLEAIILAVLDNPNTYVFDLVYQNKSILFNLLSDFLKNTANTLPFLQEQFDIVNHLFANEAIFDLFSNADFVEKIADLFLAKQKVQEVNNDGTKSTKIVDSILVATLKGLVGDQLSSITELLNIYIFENEFLNRNDSNSSVKKQQTDSLKNLFSVIGDILSETNVNKITLHAVKNNELLSLVETASTLKIKHLNVQYKVLVDKFELKNSFIKELLNFFPDTKDITPTIKKVLFESENYKTLRKKYENEGFPGYHWAKFIVPGTFNSAENTFYSAIDKTKSIRDLFADMLFGKSLESVNDSDSFIKINGSFTLKYHGDNLNLLPNYHSLITKNVGYQIVNVNFHIDARLLTAELQNTVFSNPKPVIKSPVELSKSLFEVWKTIFENSVNQILKKEYTFKDNLKFFPFKADGSSRLEFDLSKPDQRVIPFAFVDGYQFQLKKELIPNKETKKEANSSPVLKLYDAVKRNDRQYRPNHHHDDLRNYPSLKSQLELILNLGDKLKANNDFIDDTVVNALQYKTSFKSTLKVNSLGIPINLFFFTLWLKFNLEIPIDGSLTLTSVNVVFPYSLYDTSSNEFTRIVDRLNFTDTNFYLKDAFPNFWFVGF" }, "paralog": false, "identity": 0.9755555555555555, "score": 1716.0, "evalue": 0.0, "description": "Internal stop codon at 101775. Nonsense mutation around 101775.", "psc": { "uniref90_id": "UniRef90_P47321", "product": "Uncharacterized protein MG075", "uniref50_id": "UniRef50_P47321", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47321", "UniRef:UniRef50_P47321", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47321", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47321" ], "product": "Uncharacterized protein MG075" } }, "id": "JPCNHPFPBM_222", "locus": "GCA000292505_00183" }, { "type": "cds", "sequence": "contig_1", "start": 102083, "stop": 102394, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED933E1", "UniRef:UniRef90_UPI001ED933E1" ], "nt": "GTGAAATTTAAGCGATCGACTATCCTTGTAAATTCATTTGAACTAGTATCATACAAACTGTATGGGAAAACAACATTAACAGATGTTAATGTTAATGAACCGTCAATTGGAATTTCTAAGTTAAATTTAAGTCACAATGTGAAAAAGAAAAGGTCAATAGGAATTCCTAAACTGTTAACTTTTAGAGTAGATTTAAAACTAGTTTTATATTGCAAAGCATTAACAACAGTGTCATCTATAAAATCATTATTAGCTTTAAGCTTATCACCAAGGTTTAATATAAGTTCTAGTTGGGATTTAAGACTAGGATAG", "aa": "MKFKRSTILVNSFELVSYKLYGKTTLTDVNVNEPSIGISKLNLSHNVKKKRSIGIPKLLTFRVDLKLVLYCKALTTVSSIKSLLALSLSPRFNISSSWDLRLG", "aa_hexdigest": "bd4523ecc6f9656075bfb34839706a3d", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001ED933E1", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.99, "score": 192.0, "evalue": 3.86e-62, "valid": true, "uniref50_id": "UniRef50_UPI001ED933E1" }, "pscc": { "uniref50_id": "UniRef50_UPI001ED933E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED933E1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11558.601499999984, "isoelectric_point": 10.294843482971189 }, "id": "JPCNHPFPBM_223", "locus": "GCA000292505_00184" }, { "type": "cds", "sequence": "contig_1", "start": 102449, "stop": 102865, "strand": "-", "frame": 3, "gene": null, "product": "MPN214 family protein", "db_xrefs": [ "GO:0005886", "RefSeq:WP_009885931.1", "SO:0001217", "UniParc:UPI0000139204", "UniRef:UniRef100_P47322", "UniRef:UniRef50_P47322", "UniRef:UniRef90_P47322" ], "nt": "ATGGTTTTAAATCAAAACAAAAACAGTAATAAAGCTGAGTATGGTGGGATAGTTGTTAGCGTATTTTATCTAATCCTTTTCTTTTTGATCTTAAACATTACTATTTATTTTCACAAGTCAACCAATTTTACAGTGGTTGTTAAAAATTCAGTACTAACTAGCTTTTTTGTTAACTTATTACTTGTTTGTTTACAGGGGATATTCCGTTTAAAAACTTGTGATGGAATGCGGTATGAAATTAGTAAGTTTAACAGATATCTTAAGTTAGGTAGTGTTTATGCAAAACCATTAGTTAGTTTTAATCAATATCAAGATCAAAGTGCAACTTACAGACAAAAAACTAGTGGGTTTTGGTGGATGAACTTGATTGTTTATCTAGTTGGTAGTTTAGTTAGTGGTTTAGTTTCATTACTCTAG", "aa": "MVLNQNKNSNKAEYGGIVVSVFYLILFFLILNITIYFHKSTNFTVVVKNSVLTSFFVNLLLVCLQGIFRLKTCDGMRYEISKFNRYLKLGSVYAKPLVSFNQYQDQSATYRQKTSGFWWMNLIVYLVGSLVSGLVSLL", "aa_hexdigest": "840fe12a91719bb65803129e7947e092", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139204", "ncbi_nrp_id": "WP_009885931.1", "uniref100_id": "UniRef100_P47322", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139204", "RefSeq:WP_009885931.1", "UniRef:UniRef100_P47322" ] }, "ips": { "uniref100_id": "UniRef100_P47322", "uniref90_id": "UniRef90_P47322" }, "psc": { "uniref90_id": "UniRef90_P47322", "product": "MPN214 family protein", "uniref50_id": "UniRef50_P47322", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47322", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47322" ], "product": "Uncharacterized protein MG076" }, "genes": [], "id": "JPCNHPFPBM_224", "locus": "GCA000292505_00185" }, { "type": "cds", "sequence": "contig_1", "start": 103097, "stop": 103297, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCAAGTATATTCTTAAACGATTAGGACTAGCAGTAGTTGCGATGTTTATCGTAATGTCTATAGTCTTCTTTTTAGTGAACGCTACTGGTAATGTTCCCTTGTCAGCCACTTCTGCAAGAGATATTGCTGCAGTGCAAGCACAACTACAAGAGTTTGGGTTTAATGACCCTATTATAATTAGGTATTTTCGCTATTGA", "aa": "MFKYILKRLGLAVVAMFIVMSIVFFLVNATGNVPLSATSARDIAAVQAQLQEFGFNDPIIIRYFRY", "aa_hexdigest": "6c4c6fd0e8e51a77175d485ed3d3876c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7460.824100000001, "isoelectric_point": 9.815069770812986 }, "id": "JPCNHPFPBM_225", "locus": "GCA000292505_00186" }, { "type": "cds", "sequence": "contig_1", "start": 103373, "stop": 104116, "strand": "+", "frame": 2, "gene": "oppB", "product": "Oligopeptide transport system permease protein OppB", "db_xrefs": [ "COG:COG0601", "COG:EP", "GO:0005886", "GO:0015031", "GO:0015833", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P75554", "UniRef:UniRef90_P47323" ], "nt": "GTGTTTGCAAGAGTACCAAATACCTTATATGTGGTTTTAATCTCTTTTTTAATTGGTTCATTGCTAGGGATCTTTTTAGGGATGGTTTCAGGATTGAATAGAGGGAAGTTTTTAGATGCAGCAATTAATGTGTTGGTAGTTTTATTTGTATCTATTCCTTCATTTGTAGTGGGATTAGGGTTACTTAAACTAGCAGGATTTTTAAATCTACCACCACGGTTTATTAACTTTGATGATGCTTTTTTTAGCTTTGATCGTTTCTTGCTTGCATCAATTATCCCGATCCTTTCATTGGTCTTTTATTCATCAGCTGCTTTTACATACAGGATTAGAAATGAGGTGGTGGAAGTGATGAATCAAGACTATATTAAAACTGCAAAAAGTAAGGGACTTGGGATGTTTGCTGTAGCTAGGTATCATATCTTTAGAAACTCGATTATTCCTTCTATTCCCTTGTTTGTATTTGGAATCTCAGGTGCTTTTTCAGGTGGATTTATTATTGAGTCTTTGTTTGGAGTACAAGGGGTATCTAGGATCTTAATTGATTCAGTGCAAGTTAATGAAACTAACATGGTAATGTTTAATATCTTGTTTATCCAAGGGATTCCCTTATTAGCAAGTGTCTTTATTGAATTTATCTATGTTTTAGTTGATCCTAGAATTAGGATTGCAAATAGTTCTAATGTTAGCTTATTAACTAAGTTAAAGTTCTTAAGTTCAAGACACCAATGGTTAATGAAGTGA", "aa": "MFARVPNTLYVVLISFLIGSLLGIFLGMVSGLNRGKFLDAAINVLVVLFVSIPSFVVGLGLLKLAGFLNLPPRFINFDDAFFSFDRFLLASIIPILSLVFYSSAAFTYRIRNEVVEVMNQDYIKTAKSKGLGMFAVARYHIFRNSIIPSIPLFVFGISGAFSGGFIIESLFGVQGVSRILIDSVQVNETNMVMFNILFIQGIPLLASVFIEFIYVLVDPRIRIANSSNVSLLTKLKFLSSRHQWLMK", "aa_hexdigest": "b7c521ba66acfb3ac96f6e00bcf2a0b3", "start_type": "GTG", "rbs_motif": null, "genes": [ "oppB" ], "truncated": "both", "pseudogene": { "start": 103097, "stop": 104320, "observations": { "mutation": [ 103295, 104114 ], "stop": [ 103295, 104114 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47323", "query_cov": 0.9959514170040485, "subject_cov": 0.6044226044226044, "identity": 1.0, "score": 440.0, "evalue": 5.17e-153, "gene_start": 2, "gene_end": 247, "reference_start": 94, "reference_end": 339, "reference_sequence": "MFKYILKRLGLAVVAMFIVMSIVFFLVNATGNVPLSATSARDIAAVQAQLQEFGFNDPIIVRYFRYWAKLFSFQADALGIYYANPNQTIGEIVFARVPNTLYVVLISFLIGSLLGIFLGMVSGLNRGKFLDAAINVLVVLFVSIPSFVVGLGLLKLAGFLNLPPRFINFDDAFFSFDRFLLASIIPILSLVFYSSAAFTYRIRNEVVEVMNQDYIKTAKSKGLGMFAVARYHIFRNSIIPSIPLFVFGISGAFSGGFIIESLFGVQGVSRILIDSVQVNETNMVMFNILFIQGIPLLASVFIEFIYVLVDPRIRIANSSNVSLLTKLKFLSSRHQWLMKWNKINSDNAQNIVFNSPLHHQLLELNAIDYKTKTVQLTTEQKTALNISATANFILLGNKCLKLKTIHG" }, "paralog": false, "identity": 0.9926289926289926, "score": 761.0, "evalue": 4.09e-282, "description": "Internal stop codon at 103295, 104114. Nonsense mutation around 103295, 104114.", "psc": { "uniref90_id": "UniRef90_P47323", "gene": "oppB", "product": "Oligopeptide transport system permease protein OppB", "uniref50_id": "UniRef50_P75554", "cog_id": "COG0601", "cog_category": "EP", "go_ids": [ "GO:0005886", "GO:0015833", "GO:0015031", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47323", "UniRef:UniRef50_P75554", "COG:COG0601", "COG:EP", "GO:0005886", "GO:0015833", "GO:0015031", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P75554", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75554" ], "product": "Oligopeptide transport system permease protein OppB" } }, "id": "JPCNHPFPBM_226", "locus": "GCA000292505_00187" }, { "type": "cds", "sequence": "contig_1", "start": 104150, "stop": 104320, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTAACTCGCCACTGCACCACCAGTTATTAGAACTCAATGCAATTGATTACAAAACAAAAACAGTCCAACTAACAACTGAACAAAAAACTGCTCTCAATATCAGTGCAACTGCTAACTTTATCTTACTTGGTAACAAGTGTTTAAAACTCAAAACAATCCATGGATAG", "aa": "MFNSPLHHQLLELNAIDYKTKTVQLTTEQKTALNISATANFILLGNKCLKLKTIHG", "aa_hexdigest": "ae026fa01f44486324269dbf017cbde2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6279.291399999998, "isoelectric_point": 9.361599159240722 }, "id": "JPCNHPFPBM_227", "locus": "GCA000292505_00188" }, { "type": "cds", "sequence": "contig_1", "start": 104313, "stop": 104798, "strand": "+", "frame": 3, "gene": "oppC", "product": "Oligopeptide transport system permease protein OppC", "db_xrefs": [ "COG:COG1173", "COG:EP", "GO:0005886", "GO:0015031", "GO:0015833", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P75553", "UniRef:UniRef90_P47324" ], "nt": "ATGGATAGAAATAAAAGTTTTGACCCTAACTTATTTAAAAGGGTTGATATCAACTTAATAAAGCGAAATGATCAGCTTATTGGTAAACCAACTACCAATTCAATAGAAATTATCAAGCGCTTGTTTCAAAACAAGTGGGCCATCTTATTTTTTCTTTTAATAGTTGTTATTGTGCTATTAGCAATTATTGTGCCTTTAACTTCCCCTTTTTCAGCAGTAACTCCTGTTTCAACCAATGCCTTAGCACAAAATCTACCACCACGGTACTTATGGCATAAACCAGGTGACATTTTAGTTCATAAGATTACAGCAAGATCAATTGCTGAAATCTCTCAAGCTAGTGGAGTTTTAGTAGGAACATTACCTAGTGCAAATAGTAATCCCTTAGCAACTAATGTCCAGTATGATATTGCTCCTTTTCAACTCCAAGAATTACGTAATTATTTCCCTTTATTGGGGACTAATGGACTTGGGATTGATATTTGA", "aa": "MDRNKSFDPNLFKRVDINLIKRNDQLIGKPTTNSIEIIKRLFQNKWAILFFLLIVVIVLLAIIVPLTSPFSAVTPVSTNALAQNLPPRYLWHKPGDILVHKITARSIAEISQASGVLVGTLPSANSNPLATNVQYDIAPFQLQELRNYFPLLGTNGLGIDI", "aa_hexdigest": "9fabd8a8e5bee2a62dc6c60d492918ad", "start_type": "ATG", "rbs_motif": null, "genes": [ "oppC" ], "truncated": "3-prime", "pseudogene": { "start": 104313, "stop": 105101, "observations": { "mutation": [ 104796, 104808, 104832, 104973, 105063, 105069 ], "stop": [ 104796, 104808, 104832, 104973, 105063, 105069 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47324", "query_cov": 1.0, "subject_cov": 0.42819148936170215, "identity": 0.9940000000000001, "score": 301.0, "evalue": 3.78e-100, "gene_start": 1, "gene_end": 161, "reference_start": 1, "reference_end": 161, "reference_sequence": "MDRNKSFDPNLFKRVDINLLKRNDQLIGKPTTNSIEIIKRLFQNKWAILFFLLIVVIVLLAIIVPLTSPFSAVTPVSTNALAQNLPPRYLWHKPGDILVHKITARSIAEISQASGVLVGTLPSANSNPLATNVQYDIAPFQLQELRNYFPLLGTNGLGIDIWTLLWASVAKSLWIAVVVAIIAMVFGTIYGAVAGSFVGHMADNIMSRIIEIIDIVPSILWIIVLGATFRFGGVKQFDDSVVIFTLIFVFWTWPATTTRIYILKNKDTEYIQAAKTLGAHQIRIIFVHMLPVVFGRLAVVFVSLIPAVIGYEASLVFLGLKPATDIGLGALLNQVTSSDNVALILSSIVSFAVLTVAARTFANALNDAIDPRVVKR" }, "paralog": false, "identity": 0.9694656488549618, "score": 417.0, "evalue": 2.39e-148, "description": "Internal stop codon at 104796, 104808, 104832, 104973, 105063, 105069. Nonsense mutation around 104796, 104808, 104832, 104973, 105063, 105069.", "psc": { "uniref90_id": "UniRef90_P47324", "gene": "oppC", "product": "Oligopeptide transport system permease protein OppC", "uniref50_id": "UniRef50_P75553", "cog_id": "COG1173", "cog_category": "EP", "go_ids": [ "GO:0005886", "GO:0015833", "GO:0015031", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47324", "UniRef:UniRef50_P75553", "COG:COG1173", "COG:EP", "GO:0005886", "GO:0015833", "GO:0015031", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P75553", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75553" ], "product": "Oligopeptide transport system permease protein OppC" } }, "id": "JPCNHPFPBM_228", "locus": "GCA000292505_00189" }, { "type": "cds", "sequence": "contig_1", "start": 104862, "stop": 104975, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTATTTGGAACCATTTATGGAGCGGTTGCTGGAAGCTTTGTTGGACATATGGCTGATAACATTATGAGTAGGATCATTGAGATTATTGATATAGTCCCTTCTATTCTTTGA", "aa": "MVFGTIYGAVAGSFVGHMADNIMSRIIEIIDIVPSIL", "aa_hexdigest": "4936c367a942cc35c6979e56c0d499a9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3951.6727999999994, "isoelectric_point": 4.535092353820801 }, "id": "JPCNHPFPBM_229", "locus": "GCA000292505_00190" }, { "type": "cds", "sequence": "contig_1", "start": 105099, "stop": 105443, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAACAAAGATACAGAGTACATCCAAGCAGCTAAGACCTTAGGGGCACACCAAATCAGAATTATCTTTGTTCATATGTTACCTGTTGTATTTGGGAGATTAGCTGTTGTGTTTGTTAGTTTAATCCCAGCAGTTATTGGTTATGAAGCTTCCTTAGTTTTCCTTGGGTTAAAACCAGCTACTGATATTGGCTTAGGGGCACTTTTAAACCAAGTAACTTCAAGTGATAATGTAGCTTTAATCTTAAGTTCGATTGTTAGCTTTGCAGTTTTAACAGTAGCAGCTAGAACATTTGCTAATGCTTTAAATGATGCAATTGACCCTAGGGTTGTAAAACGATAA", "aa": "MKNKDTEYIQAAKTLGAHQIRIIFVHMLPVVFGRLAVVFVSLIPAVIGYEASLVFLGLKPATDIGLGALLNQVTSSDNVALILSSIVSFAVLTVAARTFANALNDAIDPRVVKR", "aa_hexdigest": "b1cfb3aa042724869febbd9f66897e22", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12190.27969999998, "isoelectric_point": 9.59697322845459 }, "id": "JPCNHPFPBM_230", "locus": "GCA000292505_00191" }, { "type": "cds", "sequence": "contig_1", "start": 105445, "stop": 106557, "strand": "+", "frame": 1, "gene": "oppD", "product": "Oligopeptide transport ATP-binding protein OppD", "db_xrefs": [ "COG:COG0444", "COG:EP", "EC:7.4.2.6", "GO:0005524", "GO:0005886", "GO:0015031", "GO:0015833", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P47325", "UniRef:UniRef90_P47325" ], "nt": "ATGGCACTTAAAAGAAGTAATTTCTTTGTTGATAAAGACCAACAACTAAAGGATAATTTGATCTTAGACATCACTGATTTACATGTTAACTTTAAGGTTAAAGATGGGATCTTACATGCTGTTAGAGGGATTGATCTTAAGGTAGAGAGGGGTAGTATTGTAGGGATTGTAGGTGAATCAGGCAGCGGTAAATCAGTGAGTGTTAAATCAATTATTGGTTTTAATGACAATGCACAAACTAAAGCCAAACTGATGAACTTTAAAAACGTTGATATTACCAAACTAAAGAAACACCAGTGGAAGTATTATAGAGGGACATATGTCTCTTATATTTCCCAAGACCCATTGTTTTCTCTAAACCCAACAATGACGATAGGAAAACAAGTAAAAGAAGCGATTTATGTGGCTTCAAAAAGAAGGTATTTCCAAGCTAAATCAGACTTAAAATTTGCTTTATCAAATAAGGAGATTGACAAAAAAACTTATAAAAGTAAACTAAAAGAGATCAAACAAACCTACCAACAAAAAATAAAACCTATCAATGTAGAGAAAAAAACCTTAGAGATCCTGCAGTTCATTGGTATTAATGATGCCAAGAAACGTTTAAAGGCATTCCCAAGTGAGTTTTCAGGAGGGATGAGACAGAGAATTGTGATTGCTATTGCAGTAGCAACTGAACCTGATTTAATTATTGCTGATGAACCTACTACTGCACTTGATGTAACTATTCAAGCTAAGGTATTAACTTTAATTAAACAACTCCGTGATTTACTTAATATCACTATTATCTTTATTAGTCACAATATCTCTTTAATTGCTAATTTCTGTGACTTTGTTTATGTTATGTATGCAGGGAAAATTGTAGAACAGGGTCTTGTTGAAGAGATCTTTACAAATCCACTCCATCCCTATACATGGGCATTGATTTCTTCAATTCCTGAACAGAAAGATAAAAACAAACCACTAACTTCTATCCCTGGAGTTATTCCTAACATGTTAACCCCACCAAAGGGTGATGCTTTCGCTAGTAGAAACCAATATGCTCTAGCAATTGACTTTGAATACCATCCACCCTTTTTTGAAGTTACTAAAACCCATAAAGCAGCAACTTGA", "aa": "MALKRSNFFVDKDQQLKDNLILDITDLHVNFKVKDGILHAVRGIDLKVERGSIVGIVGESGSGKSVSVKSIIGFNDNAQTKAKLMNFKNVDITKLKKHQWKYYRGTYVSYISQDPLFSLNPTMTIGKQVKEAIYVASKRRYFQAKSDLKFALSNKEIDKKTYKSKLKEIKQTYQQKIKPINVEKKTLEILQFIGINDAKKRLKAFPSEFSGGMRQRIVIAIAVATEPDLIIADEPTTALDVTIQAKVLTLIKQLRDLLNITIIFISHNISLIANFCDFVYVMYAGKIVEQGLVEEIFTNPLHPYTWALISSIPEQKDKNKPLTSIPGVIPNMLTPPKGDAFASRNQYALAIDFEYHPPFFEVTKTHKAAT", "aa_hexdigest": "b87f297b52a9b1034127f2a9af8514c8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47325", "query_cov": 1.0, "subject_cov": 0.9203980099502488, "identity": 1.0, "score": 706.0, "evalue": 3.85e-256, "valid": true, "gene": "oppD", "product": "Oligopeptide transport ATP-binding protein OppD", "ec_ids": [ "7.4.2.6" ], "uniref50_id": "UniRef50_P47325", "cog_id": "COG0444", "cog_category": "EP", "go_ids": [ "GO:0005886", "GO:0005524", "GO:0016887", "GO:0015833", "GO:0015031" ] }, "pscc": { "uniref50_id": "UniRef50_P47325", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47325" ], "product": "Oligopeptide transport ATP-binding protein OppD" }, "genes": [ "oppD" ], "id": "JPCNHPFPBM_231", "locus": "GCA000292505_00192" }, { "type": "cds", "sequence": "contig_1", "start": 106653, "stop": 108002, "strand": "+", "frame": 3, "gene": "oppF", "product": "Oligopeptide transport ATP-binding protein OppF", "db_xrefs": [ "COG:COG4172", "COG:Q", "EC:7.4.2.6", "GO:0005524", "GO:0005886", "GO:0015031", "GO:0015833", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P75551", "UniRef:UniRef90_P47326" ], "nt": "ATGGAAAACCAAAACACAAAAAAACCACTTGTTAATGTTAAGGCTTTGAGCATGATGTTCAAGGTCAGAGGAACTCTTTTTAAAGCCCTTGATGAAATTGGTTTTACTGTTAATGAAGGGGACTTCTTTGGAGTTATTGGTGAGAGTGGTAGTGGTAAATCAACCACGGGAAAATGTTTGATTAGATTAAACATTCCTAGTGGTGGAAAGATTGAGATTGCCAACCACTTACTCTCAGGAAAAAAACTTACTAAAGAGAATAACCAGTGGTTAAAACAAAACATCCAAATGGTGTTTCAAGACCCTTATTCATCTATTAACCCTACTAAAAATGTGCTAACTGTGATTTCAGAACCGCTGGTAATTAGTAAAACTGTTTTTGGGGAAACAAAACAATACTTAAAGAGTTTGCAAAAGCTCTCTTTTAAAGTAAAGAAAACATTGTTAAGGAATGATATTGAACTTGAAACCAAGTTTCACAATAACTTTTTTAAAACCGTTATTAAGCAAATTAATGAATCATTGTTTAACTTTGAAGATCTTGATTACAAGGATTTAAAACCATCACATTTAAGGCAAAGAATCATAAATGAAACAGATAAATTCATTGAAAAAATTAGAAGTGAGTTTGCCCTTTTTTATGATTTTTATGCTAACCAATCAGTACCCTTGCAAAAGGCATTAGATGATGCGAATTCCTCTTTAACACCATCTAGTGTTATTGAGTTAAAAAACCAGTTAAAAGCATTACAAAAACAAGCAAAGATTTCAAAGGCAGCATGGGATATTTTACAAGCCCTAAAGCAAAACCAAAAGGAGTTGAAAGATTATGAAAACTATGTCCATTTTGAACTCCAAAAAAAGCCACGAATCTATCTTAATACCTGGCTTTTAACAACCAAAAGCTACATTAAAGATTCCAAGCAAAACATGCAGCTTACTGATGATATCTTTGCTTTTTCATACAACAGTATGGTTGACAAGAAAAGAAACTTGGTTTTAATTCTTTCTAAATACTATAAGCTGTTACCTTATTTCTATGACCAATCAGTATTTGATAATGCTGATCAATTTGATGAAATTGCTAACCTTATCTTTTTTGATTTAGTTGAAACATTGCTTGGTGTAACTAGTTTATTTAATGATGCATTAGCAGCTGATAAAGTCCCACTAATTAAGTTTGCTAAGTTCTTAAATAAGTTATGTGACTTGCGCTTTTTAACCTTAAAAAAGAGCTTTAAAAAAACAAGAGTAAGTTGTAGCTTTAGTTTTAACAGTGAACCTGAAATCTTGTTTGCCAACAGCTGCTATGATTTGCAACAAATGCCTCAAATCATTAAACCCTTTTGA", "aa": "MENQNTKKPLVNVKALSMMFKVRGTLFKALDEIGFTVNEGDFFGVIGESGSGKSTTGKCLIRLNIPSGGKIEIANHLLSGKKLTKENNQWLKQNIQMVFQDPYSSINPTKNVLTVISEPLVISKTVFGETKQYLKSLQKLSFKVKKTLLRNDIELETKFHNNFFKTVIKQINESLFNFEDLDYKDLKPSHLRQRIINETDKFIEKIRSEFALFYDFYANQSVPLQKALDDANSSLTPSSVIELKNQLKALQKQAKISKAAWDILQALKQNQKELKDYENYVHFELQKKPRIYLNTWLLTTKSYIKDSKQNMQLTDDIFAFSYNSMVDKKRNLVLILSKYYKLLPYFYDQSVFDNADQFDEIANLIFFDLVETLLGVTSLFNDALAADKVPLIKFAKFLNKLCDLRFLTLKKSFKKTRVSCSFSFNSEPEILFANSCYDLQQMPQIIKPF", "aa_hexdigest": "e296e99498ba69c322aaa62c9ce285d6", "start_type": "ATG", "rbs_motif": null, "genes": [ "oppF" ], "truncated": "3-prime", "pseudogene": { "start": 106653, "stop": 108248, "observations": { "mutation": [ 108000 ], "stop": [ 108000 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47326", "query_cov": 1.0, "subject_cov": 0.5294811320754716, "identity": 1.0, "score": 868.0, "evalue": 8.27e-312, "gene_start": 1, "gene_end": 449, "reference_start": 1, "reference_end": 449, "reference_sequence": "MENQNTKKPLVNVKALSMMFKVRGTLFKALDEIGFTVNEGDFFGVIGESGSGKSTTGKCLIRLNIPSGGKIEIANHLLSGKKLTKENNQWLKQNIQMVFQDPYSSINPTKNVLTVISEPLVISKTVFGETKQYLKSLQKLSFKVKKTLLRNDIELETKFHNNFFKTVIKQINESLFNFEDLDYKDLKPSHLRQRIINETDKFIEKIRSEFALFYDFYANQSVPLQKALDDANSSLTPSSVIELKNQLKALQKQAKISKAAWDILQALKQNQKELKDYENYVHFELQKKPRIYLNTWLLTTKSYIKDSKQNMQLTDDIFAFSYNSMVDKKRNLVLILSKYYKLLPYFYDQSVFDNADQFDEIANLIFFDLVETLLGVTSLFNDALAADKVPLIKFAKFLNKLCDLRFLTLKKSFKKTRVSCSFSFNSEPEILFANSCYDLQQMPQIIKPFWEKLFNEQNYQKIIDSVSRLNVMIANYITKAFEIKKTIDEKLREFKQQNLALKKAYSANKKSEANKASINELKVNLKTLKKQLKQEKNTTKKQSKKELKPLLKEHHTALKLHDEFNHDLRKWFKKLNFMVKKYNRLENSQKKFCLVKKLKALFKKQDETLQSELRPKLKTFGVINFEYKRAVKESNVFRLVHFAKNIFKPFLFFNLTKIFMRNKVYEALDSVGLKREHAYRYPHEFSGGQRQRIAIARALITKPKLIIADELISALDVSIQAQVINILKDLAKKHNLTVLFIAHDLSMVQTVCNRLIIMHRGKIVERGSVDEIFSNPVHPYTRSLIKASPKLSKINVDLASFDENFTYDSDYSLTNMPFYIKVPNSEEHELYCTQKQFDSWIKEATPIN" }, "paralog": false, "identity": 0.9962335216572504, "score": 1034.0, "evalue": 0.0, "description": "Internal stop codon at 108000. Nonsense mutation around 108000.", "psc": { "uniref90_id": "UniRef90_P47326", "gene": "oppF", "product": "Oligopeptide transport ATP-binding protein OppF", "ec_ids": [ "7.4.2.6" ], "uniref50_id": "UniRef50_P75551", "cog_id": "COG4172", "cog_category": "Q", "go_ids": [ "GO:0005886", "GO:0005524", "GO:0016887", "GO:0015833", "GO:0015031" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47326", "EC:7.4.2.6", "UniRef:UniRef50_P75551", "COG:COG4172", "COG:Q", "GO:0005886", "GO:0005524", "GO:0016887", "GO:0015833", "GO:0015031" ] }, "pscc": { "uniref50_id": "UniRef50_P75551", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75551" ], "product": "Oligopeptide transport ATP-binding protein OppF" } }, "id": "JPCNHPFPBM_232", "locus": "GCA000292505_00193" }, { "type": "cds", "sequence": "contig_1", "start": 108066, "stop": 109172, "strand": "+", "frame": 3, "gene": "oppF", "product": "Oligopeptide transport ATP-binding protein OppF", "db_xrefs": [ "COG:COG4172", "COG:Q", "EC:7.4.2.6", "GO:0005524", "GO:0005886", "GO:0015031", "GO:0015833", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P75551", "UniRef:UniRef90_P47326" ], "nt": "ATGATTGCAAATGACATTACCAAAGCTTTTGAAATTAAAAAAACTATTGATGAAAAACTAAGGGAGTTTAAACAACAAAATTTAGCTTTAAAAAAAGCTTATTCAGCTAACAAGAAAAGTGAGGCAAACAAAGCTTCCATTAATGAGTTAAAAGTCAATTTAAAAACACTTAAAAAACAGCTTAAACAAGAGAAAAATACTACTAAAAAACAATCAAAAAAGGAATTAAAACCACTTTTAAAAGAACACCATACTGCTTTAAAACTCCATGATGAGTTTAACCATGATTTACGCAAGTGGTTCAAAAAACTTAACTTTATGGTTAAGAAATACAACCGACTGGAAAACAGCCAGAAAAAGTTTTGTTTAGTTAAAAAGTTAAAAGCGCTTTTCAAAAAACAGGATGAAACACTACAAAGTGAATTAAGACCAAAACTAAAAACATTTGGTGTAATTAACTTTGAGTACAAACGTGCAGTCAAAGAGTCCAATGTCTTTCGATTGATGCATTTTGCTAAAAATATCTTTAAACCATTCTTGTTTTTTAACCTCACCAAGATTTTTATGAGAAATAAGGTCTATGAAGCACTCGATAGTGTTGGTTTAAAAAGAGAACATGCTTACAGATACCCCCATGAATTTTCAGGCGGACAAAGACAAAGAATTGCTATTGCCCGTGCTTTAATCACTAAACCCAAACTGATTATTGCAGATGAATTGATTAGTGCACTTGATGTTTCTATCCAAGCCCAAGTTATTAACATCTTGAAAGACTTGGCTAAAAAACACAACTTAACTGTGCTTTTCATTGCCCATGATTTATCAATGGTGCAAACTGTTTGTAACCGTTTGATCATTATGCATAGGGGCAAGATTGTTGAACGGGGCAGTGTGGATGAGATCTTTTCAAATCCAGTTCATCCCTACACCCGTTCCCTAATAAAAGCATCTCCTAAGTTAAGCAAAATCAATGTTGATCTCGCTTCTTTTGATGAAAACTTCACTTATGATAGTGATTATTCACTAACCAATATGCCCTTTTATATTAAAGTTCCTAACAGTGAAGAACATGAACTTTACTGTACTCAAAAGCAATTTGATAGTTGA", "aa": "MIANDITKAFEIKKTIDEKLREFKQQNLALKKAYSANKKSEANKASINELKVNLKTLKKQLKQEKNTTKKQSKKELKPLLKEHHTALKLHDEFNHDLRKWFKKLNFMVKKYNRLENSQKKFCLVKKLKALFKKQDETLQSELRPKLKTFGVINFEYKRAVKESNVFRLMHFAKNIFKPFLFFNLTKIFMRNKVYEALDSVGLKREHAYRYPHEFSGGQRQRIAIARALITKPKLIIADELISALDVSIQAQVINILKDLAKKHNLTVLFIAHDLSMVQTVCNRLIIMHRGKIVERGSVDEIFSNPVHPYTRSLIKASPKLSKINVDLASFDENFTYDSDYSLTNMPFYIKVPNSEEHELYCTQKQFDS", "aa_hexdigest": "2624e5e67d30f713ef96103cc23c3444", "start_type": "ATG", "rbs_motif": null, "genes": [ "oppF" ], "truncated": "both", "pseudogene": { "start": 107766, "stop": 109199, "observations": { "mutation": [ 108000, 109170 ], "stop": [ 108000, 109170 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47326", "query_cov": 1.0, "subject_cov": 0.4339622641509434, "identity": 0.995, "score": 651.0, "evalue": 1.59e-227, "gene_start": 1, "gene_end": 368, "reference_start": 472, "reference_end": 839, "reference_sequence": "MENQNTKKPLVNVKALSMMFKVRGTLFKALDEIGFTVNEGDFFGVIGESGSGKSTTGKCLIRLNIPSGGKIEIANHLLSGKKLTKENNQWLKQNIQMVFQDPYSSINPTKNVLTVISEPLVISKTVFGETKQYLKSLQKLSFKVKKTLLRNDIELETKFHNNFFKTVIKQINESLFNFEDLDYKDLKPSHLRQRIINETDKFIEKIRSEFALFYDFYANQSVPLQKALDDANSSLTPSSVIELKNQLKALQKQAKISKAAWDILQALKQNQKELKDYENYVHFELQKKPRIYLNTWLLTTKSYIKDSKQNMQLTDDIFAFSYNSMVDKKRNLVLILSKYYKLLPYFYDQSVFDNADQFDEIANLIFFDLVETLLGVTSLFNDALAADKVPLIKFAKFLNKLCDLRFLTLKKSFKKTRVSCSFSFNSEPEILFANSCYDLQQMPQIIKPFWEKLFNEQNYQKIIDSVSRLNVMIANYITKAFEIKKTIDEKLREFKQQNLALKKAYSANKKSEANKASINELKVNLKTLKKQLKQEKNTTKKQSKKELKPLLKEHHTALKLHDEFNHDLRKWFKKLNFMVKKYNRLENSQKKFCLVKKLKALFKKQDETLQSELRPKLKTFGVINFEYKRAVKESNVFRLVHFAKNIFKPFLFFNLTKIFMRNKVYEALDSVGLKREHAYRYPHEFSGGQRQRIAIARALITKPKLIIADELISALDVSIQAQVINILKDLAKKHNLTVLFIAHDLSMVQTVCNRLIIMHRGKIVERGSVDEIFSNPVHPYTRSLIKASPKLSKINVDLASFDENFTYDSDYSLTNMPFYIKVPNSEEHELYCTQKQFDSWIKEATPIN" }, "paralog": false, "identity": 0.9916142557651991, "score": 880.0, "evalue": 4.52e-320, "description": "Internal stop codon at 108000, 109170. Nonsense mutation around 108000, 109170.", "psc": { "uniref90_id": "UniRef90_P47326", "gene": "oppF", "product": "Oligopeptide transport ATP-binding protein OppF", "ec_ids": [ "7.4.2.6" ], "uniref50_id": "UniRef50_P75551", "cog_id": "COG4172", "cog_category": "Q", "go_ids": [ "GO:0005886", "GO:0005524", "GO:0016887", "GO:0015833", "GO:0015031" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47326", "EC:7.4.2.6", "UniRef:UniRef50_P75551", "COG:COG4172", "COG:Q", "GO:0005886", "GO:0005524", "GO:0016887", "GO:0015833", "GO:0015031" ] }, "pscc": { "uniref50_id": "UniRef50_P75551", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75551" ], "product": "Oligopeptide transport ATP-binding protein OppF" } }, "id": "JPCNHPFPBM_233", "locus": "GCA000292505_00194" }, { "type": "cds", "sequence": "contig_1", "start": 109255, "stop": 109668, "strand": "+", "frame": 1, "gene": "rplK", "product": "50S ribosomal protein L11", "db_xrefs": [ "COG:COG0080", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:0070180", "KEGG:K02867", "RefSeq:WP_009885638.1", "SO:0001217", "UniParc:UPI0000165F74", "UniRef:UniRef100_P47327", "UniRef:UniRef50_P47327", "UniRef:UniRef90_P47327" ], "nt": "ATGGCTAAAAAAACAGTTACAAGAATCGCTAAGATTAACCTAATTGGCGGACAAGCAAAACCTGGCCCTGCGCTTGCTTCTGTAGGGATTAATATGGGTGAGTTTACCAAACAATTTAATGAAAAAACCAAGGATAGACAAGGTGAAACGATCCCTTGTATAATCACTGCTTTTAACGATAAATCATTTACTTTTGTCTTAAAAACTACCCCTGTTAGTAACTTAATTAAACAAGCTGCTAAACTAGAAAAAGGTGCTAAAAATGCAAAAACTATTGTTGGAAAAATCTCCTTACAACAAGCTAAGGAGATTGCGCAATACAAGTTAGTTGATCTTAATGCTAACACAGTTGAAGCAGCATTAAAAATGGTGTTAGGTACAGCTAAACAGATGGGAATAGAGGTAACTGATTAA", "aa": "MAKKTVTRIAKINLIGGQAKPGPALASVGINMGEFTKQFNEKTKDRQGETIPCIITAFNDKSFTFVLKTTPVSNLIKQAAKLEKGAKNAKTIVGKISLQQAKEIAQYKLVDLNANTVEAALKMVLGTAKQMGIEVTD", "aa_hexdigest": "19bb1803ec9bd00a603f99a2e03a8a04", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000165F74", "ncbi_nrp_id": "WP_009885638.1", "uniref100_id": "UniRef100_P47327", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000165F74", "RefSeq:WP_009885638.1", "UniRef:UniRef100_P47327" ] }, "ips": { "uniref100_id": "UniRef100_P47327", "uniref90_id": "UniRef90_P47327" }, "psc": { "uniref90_id": "UniRef90_P47327", "gene": "rplK", "product": "50S ribosomal protein L11", "uniref50_id": "UniRef50_P47327", "cog_id": "COG0080", "cog_category": "J", "kegg_orthology_id": "K02867", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:0070180" ] }, "pscc": { "uniref50_id": "UniRef50_P47327", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47327" ], "product": "Large ribosomal subunit protein uL11" }, "genes": [ "rplK" ], "id": "JPCNHPFPBM_234", "locus": "GCA000292505_00195" }, { "type": "cds", "sequence": "contig_1", "start": 109668, "stop": 109985, "strand": "+", "frame": 3, "gene": "rplA", "product": "50S ribosomal protein L1", "db_xrefs": [ "COG:COG0081", "COG:J", "GO:0000049", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0006417", "GO:0015934", "GO:0019843", "KEGG:K02863", "SO:0001217", "UniRef:UniRef50_P47328", "UniRef:UniRef90_P47328" ], "nt": "ATGAAAAAACTATCAAAAAGGATGCAAGCTGTTACCAAGCTCATTGATAAAAACAAACTTTATCCTATCCAAGAAGCATTTGAATTAATTAAAAAAACAGCAATTACTAAGTTTGTCAGTTCAGTTGATATTGCTGTTAGTTTAAACCTTGATACTACTAAAGCTGAACAACAGTTAAGAGGTGCAATTGCTTTTCCTTTTAGTATTGGTAAATCTATCAGAATTTTAGCTATCACTGATGATGAGAAAAAAGCTAGTGAAGCAGGTGCTGATTTTGTTGGTGGGCTTGATAAGATAGAAGCGATAAAAAATGGCTGA", "aa": "MKKLSKRMQAVTKLIDKNKLYPIQEAFELIKKTAITKFVSSVDIAVSLNLDTTKAEQQLRGAIAFPFSIGKSIRILAITDDEKKASEAGADFVGGLDKIEAIKNG", "aa_hexdigest": "a656919bcd0b3a095363b3c59fd9c835", "start_type": "ATG", "rbs_motif": null, "genes": [ "rplA" ], "truncated": "3-prime", "pseudogene": { "start": 109668, "stop": 110288, "observations": { "mutation": [ 109983 ], "stop": [ 109983 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47328", "query_cov": 1.0, "subject_cov": 0.4646017699115044, "identity": 1.0, "score": 197.0, "evalue": 4.55e-62, "gene_start": 1, "gene_end": 105, "reference_start": 1, "reference_end": 105, "reference_sequence": "MKKLSKRMQAVTKLIDKNKLYPIQEAFELIKKTAITKFVSSVDIAVSLNLDTTKAEQQLRGAIAFPFSIGKSIRILAITDDEKKASEAGADFVGGLDKIEAIKNGWLDFDLIITSPKFMGALGKLGKLLGTRGLMPNPKTETVTDDVVSAIKAYKKGKKEYRTDSFGNIHLSLGKTDTKTEHLVANAMALIDLIKSKRPSTVKGTYIKNIALTTTMGPSLKVKLPD" }, "paralog": false, "identity": 0.9951456310679612, "score": 393.0, "evalue": 5.5e-142, "description": "Internal stop codon at 109983. Nonsense mutation around 109983.", "psc": { "uniref90_id": "UniRef90_P47328", "gene": "rplA", "product": "50S ribosomal protein L1", "uniref50_id": "UniRef50_P47328", "cog_id": "COG0081", "cog_category": "J", "kegg_orthology_id": "K02863", "go_ids": [ "GO:0000049", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0006417", "GO:0015934", "GO:0019843" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47328", "UniRef:UniRef50_P47328", "COG:COG0081", "COG:J", "KEGG:K02863", "GO:0000049", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0006417", "GO:0015934", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_P47328", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47328" ], "product": "Large ribosomal subunit protein uL1" } }, "id": "JPCNHPFPBM_235", "locus": "GCA000292505_00196" }, { "type": "cds", "sequence": "contig_1", "start": 110022, "stop": 110348, "strand": "+", "frame": 3, "gene": null, "product": "Ribosomal protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2P6FDS1" ], "nt": "ATGGGAGCATTAGGTAAACTAGGAAAACTATTAGGAACCAGGGGATTGATGCCAAACCCAAAAACTGAAACAGTTACTGATGATGTAGTTAGTGCTATTAAAGCTTATAAAAAGGGTAAGAAAGAATATCGAACTGATTCATTTGGCAACATCCACCTCTCTTTAGGTAAAACAGATACCAAAACTGAGCACTTGGTTGCTAATGCCATGGCTTTAATAGATTTAATTAAATCTAAACGTCCTAGCACAGTCAAAGGTACTTACATTAAAAATATTGCTTTGACAACAACAATGGGACCAAGTTTAAAAGTAAAGCTACCTGATTAA", "aa": "MGALGKLGKLLGTRGLMPNPKTETVTDDVVSAIKAYKKGKKEYRTDSFGNIHLSLGKTDTKTEHLVANAMALIDLIKSKRPSTVKGTYIKNIALTTTMGPSLKVKLPD", "aa_hexdigest": "4256e6580f77ad810c76182ec7135e66", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2P6FDS1", "query_cov": 0.9629629629629629, "subject_cov": 0.9629629629629629, "identity": 0.5870000000000001, "score": 117.0, "evalue": 5.77e-32, "valid": false, "product": "Ribosomal protein", "uniref50_id": "UniRef50_A0A2P6FDS1", "kegg_orthology_id": "K02863" }, "pscc": { "uniref50_id": "UniRef50_A0A2P6FDS1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2P6FDS1" ], "product": "Ribosomal protein" }, "genes": [], "id": "JPCNHPFPBM_236", "locus": "GCA000292505_00197" }, { "type": "cds", "sequence": "contig_1", "start": 110348, "stop": 110917, "strand": "+", "frame": 2, "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "db_xrefs": [ "COG:COG0193", "COG:J", "EC:3.1.1.29", "GO:0004045", "GO:0005737", "GO:0006412", "RefSeq:WP_009885642.1", "SO:0001217", "UniParc:UPI0000132923", "UniRef:UniRef100_P47329", "UniRef:UniRef50_P47329", "UniRef:UniRef90_P47329" ], "nt": "ATGCCCACCTATAAACTAATTGTTGGTTTAGGTAACTTAGGTAAAAAGTATGAGAAAACTCGCCATAATGCTGGTTTTATGGTGTTAGATAGACTAGCTAGTTTATTCCACTTAAACTTTGATAAAACCAACAAGTTAGGTGATTATCTTTTTATTAAAGAAAAAGCAGCAATCTTAGCAAAACCTGCTACCTTTATGAATAATAGCGGTCTTTTTGTGAAATGGTTACAAGATCACTTTCAAATTCCGCTTGCAAACATAATGATAGTCCATGATGAAATAGCGTTTGATTTGGGAGTAATTAGGCTTAAAATGCAAGGGAGTGCTAACAATCATAATGGCATAAAATCAGTAATTAGACATTTAGATACTGAACAGTTCAATCGTTTACGCTTTGGGATTAAATCACAAAATACGAGTAACATATTGCATGAACAGGTAATGAGTGAATTCCAGAATAGTGAACTGACTAAACTGGAAGTTGCGATTACAAAGTCTGTTGAACTGTTGAAGCGTTATATTGAAGGAGAAGAGTTACAAAGGTTAATGGAATATTATCATCATGGCTAG", "aa": "MPTYKLIVGLGNLGKKYEKTRHNAGFMVLDRLASLFHLNFDKTNKLGDYLFIKEKAAILAKPATFMNNSGLFVKWLQDHFQIPLANIMIVHDEIAFDLGVIRLKMQGSANNHNGIKSVIRHLDTEQFNRLRFGIKSQNTSNILHEQVMSEFQNSELTKLEVAITKSVELLKRYIEGEELQRLMEYYHHG", "aa_hexdigest": "9408931b37249c9e048343d2b12aa2c3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000132923", "ncbi_nrp_id": "WP_009885642.1", "uniref100_id": "UniRef100_P47329", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000132923", "RefSeq:WP_009885642.1", "UniRef:UniRef100_P47329" ] }, "ips": { "uniref100_id": "UniRef100_P47329", "uniref90_id": "UniRef90_P47329" }, "psc": { "uniref90_id": "UniRef90_P47329", "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "ec_ids": [ "3.1.1.29" ], "uniref50_id": "UniRef50_P47329", "cog_id": "COG0193", "cog_category": "J", "go_ids": [ "GO:0004045", "GO:0005737", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P47329", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47329" ], "product": "Peptidyl-tRNA hydrolase" }, "genes": [ "pth" ], "id": "JPCNHPFPBM_237", "locus": "GCA000292505_00198" }, { "type": "cds", "sequence": "contig_1", "start": 110910, "stop": 111122, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAGTGAAAAACAATATATAGCAGGGGTTTCAGGTGGATCTGATTCAATGCTAATGCTTAAACTTTACCAAAAGAAGATTGCTTGTGTTGTTCATGTCAATTACAACACCAGATCAACCTCATTACGTGATCAAAAGTTAGTAGAACAATACTGTCAAAAATTAAATATTCCACTGGTTGTTCACACTGTTGATCCTGATCTAGTTTGA", "aa": "MASEKQYIAGVSGGSDSMLMLKLYQKKIACVVHVNYNTRSTSLRDQKLVEQYCQKLNIPLVVHTVDPDLV", "aa_hexdigest": "9bcf35473bb65cd8ff84750fd09c6bd0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7869.1032000000005, "isoelectric_point": 8.640069007873539 }, "id": "JPCNHPFPBM_238", "locus": "GCA000292505_00199" }, { "type": "cds", "sequence": "contig_1", "start": 111249, "stop": 111347, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGCTGGATGCAAAAAAACGTGCTGTTTACTATGGGATTAAAACCAGGTGTGAATTGTATGGTTTGAAAATCTACCGTCCCCTAATGAAATACTGA", "aa": "MQLDAKKRAVYYGIKTRCELYGLKIYRPLMKY", "aa_hexdigest": "349c372203ed58aa655e24dde8590348", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3913.719500000002, "isoelectric_point": 9.843049049377441 }, "id": "JPCNHPFPBM_239", "locus": "GCA000292505_00200" }, { "type": "cds", "sequence": "contig_1", "start": 111438, "stop": 111782, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGGTTAGAGATAGAAAAATGGTCTAAAATCGAAAAAGAACAGTTTTATATTGCTATCTGTGCAATGAACAAAACAATTGCTCAAAAACTGTTTGTATTAATGAAAAAAGCTAAAAAATGGTTATTACAACCTGATGTTAGGGAATTGAAACGGTTTTCAATTATTGATCAAAAACAGTTAATTTATAGCTATCTTATCTATCACAAGATTAATGTTAATGGTGAGAAAATTGATGCTATCCTTGATTTTATCCAACCTAGTCAACAAAAACAATACCGCTTGCAAAACGATATCTTTTTAATGGTGAAAAACCAGTGTTTAGCACTTTTATACAAAAGCTAA", "aa": "MRLEIEKWSKIEKEQFYIAICAMNKTIAQKLFVLMKKAKKWLLQPDVRELKRFSIIDQKQLIYSYLIYHKINVNGEKIDAILDFIQPSQQKQYRLQNDIFLMVKNQCLALLYKS", "aa_hexdigest": "e11f65335f95187cde31ac200bc29b48", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13758.283499999985, "isoelectric_point": 9.611091804504394 }, "id": "JPCNHPFPBM_240", "locus": "GCA000292505_00201" }, { "type": "cds", "sequence": "contig_1", "start": 111783, "stop": 112718, "strand": "+", "frame": 3, "gene": "hprK", "product": "HPr(Ser) kinase/phosphatase", "db_xrefs": [ "COG:COG1493", "COG:T", "EC:2.7.11.-", "EC:2.7.4.-", "GO:0000155", "GO:0000160", "GO:0000287", "GO:0004674", "GO:0004712", "GO:0005524", "GO:0005829", "GO:0006109", "KEGG:K06023", "RefSeq:WP_014894566.1", "SO:0001217", "UniParc:UPI00027B3404", "UniRef:UniRef100_UPI00027B3404", "UniRef:UniRef50_P75548", "UniRef:UniRef90_P47331" ], "nt": "ATGAAACATTTAACTGTCAAAGCATTAGTTTTGCAATTTAATGATTGTATCCAACTCATTGATGGTAAAAACAACATAGATAATGTGATTACTATCCCTGGGTTAAAAAGGAGTGTTTTTGAACTATTAGGACTATTTTGTAAGCCAATTGGTTCAGTTGCTATTTTAGGTAAACGTGAATTTATTTTTTTAAATCAAAAGCCAGTTGAGCAACAGAAAAAGATTATTGCAAACCTCTTGAAACTCAAACCACCTGCAGTTATTCTAACCAAGTCATTTGTTGATTGTGGTGTTTTGTTAGCTGTTAATCAAACTTATCAAGTTCCTATTTTAAAAACTAACTTGTTTTCAACTGAACTCTCTTTTACTGTTGAAACCTATATTAACGAGCAATTTGCTACTGTTCAAAAGTTACATGGAGTTTTACTTGAAATCTTTGGTGTTGGGGTATTTTTAGAAGGAAAGAGTGGGATTGGTAAATCTGAAAGTGCTTTAGATTTAATTAATAAAAACCACCTTCTAATAGGTGATGATGCTATTGAGATCTACAGATTAGGCAATAGGTTATTTGGTAGAGCACAAGCACTGGCAAAAGGCTTTATGGAGATTAGAGGTCTTGGCATCATTAATATTGAACGTGCTTATGGGTTACAGATTACAAAAGAACAAACTGAAATCCAACTAGCAATTAGTTTGTTGAGTTTAGAGGAGAAAAACAACGCTAGTTTTGAGCGCTTAGGCAGTGATTTAAAACTAAAAAATCTGCTTGGGGTTAAGATTAGTTACTATCAGATCCCTATCTCTTCAGGTAGAAAAACAAGCAAAATTATTGAAAGTGCAGTAATCGATTTTAAACTTAAGAAAAGTGGTTACAATTCAGCAAACGAGTTTATCTTAAAGCAACGTGCCATGTTAGAAGAACAAACTGATGAATAG", "aa": "MKHLTVKALVLQFNDCIQLIDGKNNIDNVITIPGLKRSVFELLGLFCKPIGSVAILGKREFIFLNQKPVEQQKKIIANLLKLKPPAVILTKSFVDCGVLLAVNQTYQVPILKTNLFSTELSFTVETYINEQFATVQKLHGVLLEIFGVGVFLEGKSGIGKSESALDLINKNHLLIGDDAIEIYRLGNRLFGRAQALAKGFMEIRGLGIINIERAYGLQITKEQTEIQLAISLLSLEEKNNASFERLGSDLKLKNLLGVKISYYQIPISSGRKTSKIIESAVIDFKLKKSGYNSANEFILKQRAMLEEQTDE", "aa_hexdigest": "83c8f8b80fcaff52f35938bfedeb6305", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3404", "ncbi_nrp_id": "WP_014894566.1", "uniref100_id": "UniRef100_UPI00027B3404", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3404", "RefSeq:WP_014894566.1", "UniRef:UniRef100_UPI00027B3404" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3404", "uniref90_id": "UniRef90_P47331" }, "psc": { "uniref90_id": "UniRef90_P47331", "gene": "hprK", "product": "HPr(Ser) kinase/phosphatase", "ec_ids": [ "2.7.11.-", "2.7.4.-" ], "uniref50_id": "UniRef50_P75548", "cog_id": "COG1493", "cog_category": "T", "kegg_orthology_id": "K06023", "go_ids": [ "GO:0000155", "GO:0000160", "GO:0000287", "GO:0004674", "GO:0004712", "GO:0005524", "GO:0005829", "GO:0006109" ] }, "pscc": { "uniref50_id": "UniRef50_P75548", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75548" ], "product": "HPr kinase/phosphorylase" }, "genes": [ "hprK" ], "id": "JPCNHPFPBM_241", "locus": "GCA000292505_00202" }, { "type": "cds", "sequence": "contig_1", "start": 112813, "stop": 113619, "strand": "+", "frame": 1, "gene": "lgt", "product": "prolipoprotein diacylglyceryl transferase", "db_xrefs": [ "COG:COG0682", "COG:M", "EC:2.5.1.145", "GO:0005886", "GO:0008961", "GO:0009249", "GO:0016020", "GO:0042158", "SO:0001217", "UniRef:UniRef50_P47332", "UniRef:UniRef90_P47332" ], "nt": "ATGTTTGTCTTTAAACTGATTTATTGTTACAAATTACAAGACAACAGTTTTTATTTTTTTATCTTTATTGCTGTTTTAACGATGGTTTTAGGCGCTCGCCTCTGGTCATTTGTAATTGGTGATTCCAATTTTGCTAACAACAATTTCTTTGATTTTCGTAACGGTGGATTGGCCATTCAGGGTGGGATTTTGTTAACCAGTATTGTCGGAGTAATCTATTTCAACTTCTTTTTAAATAGTAAGACCAATAAAACCAAAACGATTGCTGAATTACTGAATAATAAGAATGAAATAAAAGCTGTTTATGTTGAAAGAAATATCTCTGTTCTAGTGATGTTAGATCTGATTGCTCCTTGTGTTTTAATTGGTCAAGCAATTGGCAGATGGGGTAATTTTTTCAACCAAGAAGTTTATGGGTTTGCTTTAGCTGGAACAATGAATGATCCCCAAGCATTGGCTAATACCCAGTGGGGATTTTTAAAGATCTTAATGCCTAAGGTTTGGGATGGGATGTGGATTGATGGTCAGTTTCGCATTCCGCTCTTTTTAATTGAGTCATTTTTTAACACTATTTTCTTTGTGTTAATTTACTTTGTAATGGATTTTATTAGGGGAGTTAAAAGTGGCACAATTGGTTTTAGTTATTTTCTTGCTACTGGAATCATTCGTTTAATCTTGGAAAACTTTAGAGACCAAACCTTTTATTTTCAAACTTCAATAACCACTAGTATTTTGTTTATTGTCGTTGGTATTTTAGGAATTTTTTATTGCCAGTTTATCCATGTCAAATTAAGAAATTACTTCTGA", "aa": "MFVFKLIYCYKLQDNSFYFFIFIAVLTMVLGARLWSFVIGDSNFANNNFFDFRNGGLAIQGGILLTSIVGVIYFNFFLNSKTNKTKTIAELLNNKNEIKAVYVERNISVLVMLDLIAPCVLIGQAIGRWGNFFNQEVYGFALAGTMNDPQALANTQWGFLKILMPKVWDGMWIDGQFRIPLFLIESFFNTIFFVLIYFVMDFIRGVKSGTIGFSYFLATGIIRLILENFRDQTFYFQTSITTSILFIVVGILGIFYCQFIHVKLRNYF", "aa_hexdigest": "41fee8aef0cce8b48f00e2414e72cf1a", "start_type": "ATG", "rbs_motif": null, "genes": [ "lgt" ], "truncated": "both", "pseudogene": { "start": 112711, "stop": 113859, "observations": { "mutation": [ 112726, 113617 ], "stop": [ 112726, 113617 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47332", "query_cov": 1.0, "subject_cov": 0.7015706806282722, "identity": 1.0, "score": 529.0, "evalue": 3.55e-188, "gene_start": 1, "gene_end": 268, "reference_start": 35, "reference_end": 302, "reference_sequence": "MNRPSWSTAFNIGGGFPIQWYGIIVSIGIIFAILMFVFKLIYCYKLQDNSFYFFIFIAVLTMVLGARLWSFVIGDSNFANNNFFDFRNGGLAIQGGILLTSIVGVIYFNFFLNSKTNKTKTIAELLNNKNEIKAVYVERNISVLVMLDLIAPCVLIGQAIGRWGNFFNQEVYGFALAGTMNDPQALANTQWGFLKILMPKVWDGMWIDGQFRIPLFLIESFFNTIFFVLIYFVMDFIRGVKSGTIGFSYFLATGIIRLILENFRDQTFYFQTSITTSILFIVVGILGIFYCQFIHVKLRNYFWTYFFLYAFYKVAAFFTTLFLNNRKQMAQQKFAFYEKSLPNKKRSFFEMKYYNDVTTPKIYRLTDQEMKLFDKLEAVTTS" }, "paralog": false, "identity": 0.9947643979057592, "score": 764.0, "evalue": 2.2e-283, "description": "Internal stop codon at 112726, 113617. Nonsense mutation around 112726, 113617.", "psc": { "uniref90_id": "UniRef90_P47332", "gene": "lgt", "product": "prolipoprotein diacylglyceryl transferase", "ec_ids": [ "2.5.1.145" ], "uniref50_id": "UniRef50_P47332", "cog_id": "COG0682", "cog_category": "M", "go_ids": [ "GO:0005886", "GO:0008961", "GO:0009249", "GO:0016020", "GO:0042158" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47332", "EC:2.5.1.145", "UniRef:UniRef50_P47332", "COG:COG0682", "COG:M", "GO:0005886", "GO:0008961", "GO:0009249", "GO:0016020", "GO:0042158" ] }, "pscc": { "uniref50_id": "UniRef50_P47332", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47332" ], "product": "Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase" } }, "id": "JPCNHPFPBM_242", "locus": "GCA000292505_00203" }, { "type": "cds", "sequence": "contig_1", "start": 113695, "stop": 113859, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCACAACAGAAGTTTGCTTTTTATGAAAAATCACTTCCCAATAAGAAGCGTTCTTTTTTTGAAATGAAGTATTACAATGATGTAACAACACCCAAAATTTATCGTTTAACTGATCAGGAAATGAAGTTATTTGATAAATTAGAGGCAGTTACAACCAGCTAG", "aa": "MAQQKFAFYEKSLPNKKRSFFEMKYYNDVTTPKIYRLTDQEMKLFDKLEAVTTS", "aa_hexdigest": "442510d988d14817b06dcfebc104c88e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6550.491400000001, "isoelectric_point": 9.301321220397949 }, "id": "JPCNHPFPBM_243", "locus": "GCA000292505_00204" }, { "type": "cds", "sequence": "contig_1", "start": 113888, "stop": 114307, "strand": "+", "frame": 2, "gene": "rpsL", "product": "30S ribosomal protein S12", "db_xrefs": [ "COG:COG0048", "COG:J", "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0019843", "KEGG:K02950", "RefSeq:WP_010869326.1", "SO:0001217", "UniParc:UPI0000134C58", "UniRef:UniRef100_P47333", "UniRef:UniRef50_P92532", "UniRef:UniRef90_P47333" ], "nt": "ATGGCTACAATAGCGCAATTAATTAGAAAACCACGCCAAAAAAAGAAGGTTAAATCAAAGTCACCTGCACTCCATTATAACCTCAACCTTTTAAACAAAAAAACTACCAATGTTTACTCACCACTAAAGCGTGGTGTTTGCACCAGGGTTGGCACCATGACCCCCAGAAAACCTAATTCTGCACTAAGAAAGTATGCTAAGGTTAGACTTACAAATGGCTTTGAAGTACTTGCTTATATCCCAGGAGAAGGTCATAACCTACAAGAACACAGTGTTACTTTATTAAGGGGGGGTAGAGTAAAAGATCTCCCTGGAGTTAGATACCATATTGTTCGTGGTACTTTAGATACAGTTGGTGTTGACAAAAGAAGACAACAACGTTCTGCATATGGCGCTAAAAAACCAAAACCAAAATCTTAA", "aa": "MATIAQLIRKPRQKKKVKSKSPALHYNLNLLNKKTTNVYSPLKRGVCTRVGTMTPRKPNSALRKYAKVRLTNGFEVLAYIPGEGHNLQEHSVTLLRGGRVKDLPGVRYHIVRGTLDTVGVDKRRQQRSAYGAKKPKPKS", "aa_hexdigest": "15b51a4caebf9e021f3072bb40f6189b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134C58", "ncbi_nrp_id": "WP_010869326.1", "uniref100_id": "UniRef100_P47333", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134C58", "RefSeq:WP_010869326.1", "UniRef:UniRef100_P47333" ] }, "ips": { "uniref100_id": "UniRef100_P47333", "uniref90_id": "UniRef90_P47333" }, "psc": { "uniref90_id": "UniRef90_P47333", "gene": "rpsL", "product": "30S ribosomal protein S12", "uniref50_id": "UniRef50_P92532", "cog_id": "COG0048", "cog_category": "J", "kegg_orthology_id": "K02950", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "genes": [ "rpsL" ], "id": "JPCNHPFPBM_244", "locus": "GCA000292505_00205" }, { "type": "cds", "sequence": "contig_1", "start": 114324, "stop": 114791, "strand": "+", "frame": 3, "gene": "rpsG", "product": "30S ribosomal protein S7", "db_xrefs": [ "COG:COG0049", "COG:J", "GO:0000028", "GO:0000049", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0022627", "KEGG:K02992", "RefSeq:WP_009885645.1", "SO:0001217", "UniParc:UPI0000135107", "UniRef:UniRef100_P47334", "UniRef:UniRef50_P21469", "UniRef:UniRef90_P47334" ], "nt": "ATGAGAAAAAATCGTGCTTTAAAAAGAACTGTTTTACCCGATCCTGTTTTTAACAACACACTGGTTACAAGGATTATTAATGTCATCATGAAAGATGGCAAGAAGGGTTTAGCACAACGCATCTTGTATGGTGCTTTTGAGATCATTGAAAAACGCACCAACCAACAACCTTTAACTGTCTTTGAAAAAGCAGTTGATAATGTTATGCCCCGCTTAGAGTTAAAAGTGAGAAGAATTGCTGGTTCTAACTACCAAGTACCAACTGAAGTTCCCCCTGACAGAAGGATTGCTTTAGCACTAAGATGGATTGTGATCTTTGCTAACAAAAGAAATGAAAAAACAATGCTTGAACGTGTTGCTAATGAAATTATTGATGCTTTTAATAACACGGGTGCTAGTGTTAAAAAGAAGGATGATACTCACAAGATGGCAGAAGCTAACAAAGCCTTTGCCCACATGCGTTGGTAG", "aa": "MRKNRALKRTVLPDPVFNNTLVTRIINVIMKDGKKGLAQRILYGAFEIIEKRTNQQPLTVFEKAVDNVMPRLELKVRRIAGSNYQVPTEVPPDRRIALALRWIVIFANKRNEKTMLERVANEIIDAFNNTGASVKKKDDTHKMAEANKAFAHMRW", "aa_hexdigest": "c83a70d858afbe8347c2153f5149a144", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000135107", "ncbi_nrp_id": "WP_009885645.1", "uniref100_id": "UniRef100_P47334", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000135107", "RefSeq:WP_009885645.1", "UniRef:UniRef100_P47334" ] }, "ips": { "uniref100_id": "UniRef100_P47334", "uniref90_id": "UniRef90_P47334" }, "psc": { "uniref90_id": "UniRef90_P47334", "gene": "rpsG", "product": "30S ribosomal protein S7", "uniref50_id": "UniRef50_P21469", "cog_id": "COG0049", "cog_category": "J", "kegg_orthology_id": "K02992", "go_ids": [ "GO:0000028", "GO:0000049", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P21469", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P21469" ], "product": "Small ribosomal subunit protein uS7" }, "genes": [ "rpsG" ], "id": "JPCNHPFPBM_245", "locus": "GCA000292505_00206" }, { "type": "cds", "sequence": "contig_1", "start": 114801, "stop": 115016, "strand": "+", "frame": 3, "gene": "lepA", "product": "GTP-binding protein lepA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KNQ4" ], "nt": "ATGTCAAGAACAGTTGATTTAAAAAACTTCCGTAACTTTGGCATTATGGCCCATATTGATGCTGGGAAAACCACCACATCAGAACGTATTTTGTTCCATTCAGGTAGAATTCACAAGATTGGTGAAACCCATGATGGTGAATCAGTGATGGACTGGATGGAACAAGAAAAAGAAAGGGGTATTACTATCACCTCTGCAGCCACTTCAGTGAGCTGA", "aa": "MSRTVDLKNFRNFGIMAHIDAGKTTTSERILFHSGRIHKIGETHDGESVMDWMEQEKERGITITSAATSVS", "aa_hexdigest": "f943b1f57d508f2d22013d85c521b884", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3P8KNQ4", "query_cov": 0.971830985915493, "subject_cov": 0.9324324324324325, "identity": 0.7390000000000001, "score": 109.0, "evalue": 3.86e-30, "valid": false, "product": "GTP-binding protein lepA", "uniref50_id": "UniRef50_A0A3P8KNQ4" }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KNQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KNQ4" ], "product": "GTP-binding protein lepA" }, "genes": [ "lepA" ], "id": "JPCNHPFPBM_246", "locus": "GCA000292505_00207" }, { "type": "cds", "sequence": "contig_1", "start": 115035, "stop": 115160, "strand": "+", "frame": 3, "gene": null, "product": "GTP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000E2F5E1A", "UniRef:UniRef90_UPI000E2F5E1A" ], "nt": "TTGATTGACACTCCTGGCCATGTTGACTTTACAGTTGAAGTGGAGCGTAGCTTAAGGGTTTTGGATGGAGCAATTGCGGTATTGGATGCTCAAATGGGAGTAGAACCACAAACTGAAACAGTATGA", "aa": "MIDTPGHVDFTVEVERSLRVLDGAIAVLDAQMGVEPQTETV", "aa_hexdigest": "5fdc6e462382a49e41aa5e7c6538dacc", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 115029, "stop": 115247, "observations": { "mutation": [ 115158 ], "stop": [ 115158 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000E2F5E1A", "query_cov": 1.0, "subject_cov": 0.5256410256410257, "identity": 0.927, "score": 79.0, "evalue": 1.92e-18, "gene_start": 1, "gene_end": 41, "reference_start": 8, "reference_end": 48, "reference_sequence": "WRGHRVNIIDTPGHVDFTVEVERSLRVLDGAVAVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKIGADFLYSVG" }, "paralog": false, "identity": 0.8055555555555556, "score": 117.0, "evalue": 1.74e-36, "description": "Internal stop codon at 115158. Nonsense mutation around 115158.", "psc": { "uniref90_id": "UniRef90_UPI000E2F5E1A", "product": "GTP-binding protein", "uniref50_id": "UniRef50_UPI000E2F5E1A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000E2F5E1A", "UniRef:UniRef50_UPI000E2F5E1A" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000E2F5E1A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000E2F5E1A" ], "product": "GTP-binding protein" } }, "id": "JPCNHPFPBM_247", "locus": "GCA000292505_00208" }, { "type": "cds", "sequence": "contig_1", "start": 115209, "stop": 116867, "strand": "+", "frame": 3, "gene": "fusA", "product": "elongation factor G", "db_xrefs": [ "COG:COG0480", "COG:J", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006414", "GO:0032790", "KEGG:K02355", "SO:0001217", "UniRef:UniRef50_Q5SHN5", "UniRef:UniRef90_P75544" ], "nt": "ATGGATAAAACCGGTGCTAACTTTGAGCGCTCTGTTTTATCAATTCAACAACGCTTGGGAGTGAAAGCTGTTCCTATTCAATTTCCCATAGGTGCTGAAAATGATTTCAATGGCATCATTGATATCATCACTAAAAAAGCTTATTTTTTTGATGGTAATAAAGAGGAAAATGCTATTGAAAAACCAATTCCTGAACAGTATGTTGATCAAGTTGAAAAACTTTACAACAACTTAGTTGAAGAAGTTGCTAGTTTAGATGATCAACTCATGGCTGATTATCTAGATGGTAAACCAATTGAAATTGATGCAATTAAAAATGCAATTAGAAACGGGGTAATTCACTGTAAGTTTTTCCCGGTATTGTGTGGTTCAGCATTTAAAAACAAGGGAATTAAACTCTTACTTGATGCAGTGGTTGATTTTCTCCCTTCACCTGTTGATGTCCCACCTGCTAAAGCAATTGATGCAAACAACAAAGAGATATCTATTAAAGCTAGTGATGATGCTAACTTTATTGGCTTAGCATTTAAAGTTGCTACTGATCCTTTTGTTGGTAGATTAACTTTTATTAGGGTTTATGCAGGGGTTTTAAAATCTGGTTCTTATGTTAAGAATGTTAGAAAAAACAAAAAGGAAAGGGTATCACGTTTAGTGAAAATGCACGCACAAAATCGCAATGAAATTGATGAAATTAGAGCAGGGGATATCTGTGCAGTAATTGGCTTGAAAGATACTACTACTGGAGAAACTTTAACTGATGATAAGCTTGATGTGCAACTAGAAGCAATGCAATTTGCTGAACCAGTGATCTCTTTAGCAGTAGAACCTAAAACTAAAGCAGATCAGGAAAAGATGTCAATTGCTTTATCAAAACTAGCAGAAGAAGATCCTACTTTTAAAACCTTTAGTGATCCTGAAACAGGGCAAACTATTATTGCTGGAATGGGTGAGTTACACCTTGATATCTTAGTTGATAGGATGAAACGTGAATTTAAGGTAGAAGTTAACATTGGTGCACCTCAAGTTAGCTTTCGTGAAACCTTTAAATCAACTAGTGAAGTTGAGGGTAAATACATCAAACAATCAGGTGGTAGAGGTCAATATGGACATGTTAAAATCCGTTTTGAACCTAATAAAGATAAGGGCTTTGAATTTGTTGATAAGATTGTGGGCGGAAGGATTCCAAGGGAATATATTAAACCAGTTCAAGCTGGTCTTGAAAATGCAATGAATTCAGGTCCTTTAGCAGGTTACCCAATGATTGATATTAAAGCTACCTTATTTGATGGTTCTTTCCATGAAGTTGACTCAAGTGAAATGGCTTTTAAAATTGCTGCATCCTTAGCTTTAAAAGAAGCAGGTAAACAATGTAACCCAGTTTTACTTGAACCTATTATGGCAATAGAAGTTACTGTACCTGAACAGTACTTTGGGGATACAATGGGTGATATCAGTTCAAGAAGAGGGATCATTGAAGGTACTGAACAACGTGATAATGTTCAACTAATAAAAGCAAAAGTACCTTTAAAAGAGATGTTTGGTTATGCCACTGATTTACGCTCTTTTTCCCAAGGTAGGGGTAATTATGTAATGCAATTTAGCCATTATGCTGAAACTCCTAAAAGCGTTGTTAATGAGATAATTGCTAATAAAAAATAG", "aa": "MDKTGANFERSVLSIQQRLGVKAVPIQFPIGAENDFNGIIDIITKKAYFFDGNKEENAIEKPIPEQYVDQVEKLYNNLVEEVASLDDQLMADYLDGKPIEIDAIKNAIRNGVIHCKFFPVLCGSAFKNKGIKLLLDAVVDFLPSPVDVPPAKAIDANNKEISIKASDDANFIGLAFKVATDPFVGRLTFIRVYAGVLKSGSYVKNVRKNKKERVSRLVKMHAQNRNEIDEIRAGDICAVIGLKDTTTGETLTDDKLDVQLEAMQFAEPVISLAVEPKTKADQEKMSIALSKLAEEDPTFKTFSDPETGQTIIAGMGELHLDILVDRMKREFKVEVNIGAPQVSFRETFKSTSEVEGKYIKQSGGRGQYGHVKIRFEPNKDKGFEFVDKIVGGRIPREYIKPVQAGLENAMNSGPLAGYPMIDIKATLFDGSFHEVDSSEMAFKIAASLALKEAGKQCNPVLLEPIMAIEVTVPEQYFGDTMGDISSRRGIIEGTEQRDNVQLIKAKVPLKEMFGYATDLRSFSQGRGNYVMQFSHYAETPKSVVNEIIANKK", "aa_hexdigest": "cfee299aa9fc12c3d1b3536d72f233fc", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75544", "query_cov": 1.0, "subject_cov": 0.8023255813953488, "identity": 0.998, "score": 1075.0, "evalue": 0.0, "valid": true, "gene": "fusA", "product": "elongation factor G", "uniref50_id": "UniRef50_Q5SHN5", "cog_id": "COG0480", "cog_category": "J", "kegg_orthology_id": "K02355", "go_ids": [ "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006414", "GO:0032790" ] }, "pscc": { "uniref50_id": "UniRef50_Q5SHN5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5SHN5" ], "product": "Elongation factor G" }, "genes": [ "fusA" ], "id": "JPCNHPFPBM_248", "locus": "GCA000292505_00209" }, { "type": "cds", "sequence": "contig_1", "start": 116919, "stop": 117389, "strand": "+", "frame": 3, "gene": "rpsF", "product": "30S ribosomal protein S6", "db_xrefs": [ "COG:COG0360", "COG:J", "GO:0000314", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0022627", "GO:0070181", "GO:1990904", "KEGG:K02990", "SO:0001217", "UniRef:UniRef50_P47336", "UniRef:UniRef90_P47336" ], "nt": "ATGCACTACAATATCATTCTTTTAGTTGATGGTACGCTTAGTTTAGAACAAGCTAACCAAGTTGAACAAAAACACCAAAAATTGCTTGAAAAGGCAACTGAATTTAAAAGTGAATACTTAGGTTTAAAAGAGTTGGCTTACCCCATTAAAAAGCAACTTTCTGCTCACTATTACAGATGGAGTTTTCATGGTGAAAGCAATTGTACTAAGGAGTTTAAAAGAGCTGCTAACATCAATAAGCAGATAATAAGAGAGTTAATTATTAACAGAGAAAAAGACTATGGTTATTTAGGTTCAGTTAACCCTAAAAAACAACAACTGTCTTTGCAGAAGCTAACCAAGTATAATGAGATTATTGCTAGTGAAAATAATCCTGATAACCCAGATGCGCCTGTCACTTCTGGTCTAGCTTCTGTTAAACCACGGCTATCAAGAGTTGAAAAACAAAAGGAACGTGAACTTGAAAAGTGA", "aa": "MHYNIILLVDGTLSLEQANQVEQKHQKLLEKATEFKSEYLGLKELAYPIKKQLSAHYYRWSFHGESNCTKEFKRAANINKQIIRELIINREKDYGYLGSVNPKKQQLSLQKLTKYNEIIASENNPDNPDAPVTSGLASVKPRLSRVEKQKERELEK", "aa_hexdigest": "97462fe86103323364dc6241cad6a575", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpsF" ], "truncated": "3-prime", "pseudogene": { "start": 116919, "stop": 117545, "observations": { "mutation": [ 117387 ], "stop": [ 117387 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47336", "query_cov": 1.0, "subject_cov": 0.75, "identity": 1.0, "score": 301.0, "evalue": 8.18e-103, "gene_start": 1, "gene_end": 156, "reference_start": 1, "reference_end": 156, "reference_sequence": "MHYNIILLVDGTLSLEQANQVEQKHQKLLEKATEFKSEYLGLKELAYPIKKQLSAHYYRWSFHGESNCTKEFKRAANINKQIIRELIINREKDYGYLGSVNPKKQQLSLQKLTKYNEIIASENNPDNPDAPVTSGLASVKPRLSRVEKQKERELEKWTVVHQSGNFDTVQINPYRPRIKRFLQNNQQTSQANNNQPRFQNQFKKGAKP" }, "paralog": false, "identity": 0.9951923076923077, "score": 416.0, "evalue": 2.09e-150, "description": "Internal stop codon at 117387. Nonsense mutation around 117387.", "psc": { "uniref90_id": "UniRef90_P47336", "gene": "rpsF", "product": "30S ribosomal protein S6", "uniref50_id": "UniRef50_P47336", "cog_id": "COG0360", "cog_category": "J", "kegg_orthology_id": "K02990", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0022627", "GO:0070181", "GO:1990904" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47336", "UniRef:UniRef50_P47336", "COG:COG0360", "COG:J", "KEGG:K02990", "GO:0000314", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0022627", "GO:0070181", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P47336", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47336" ], "product": "Small ribosomal subunit protein bS6" } }, "id": "JPCNHPFPBM_249", "locus": "GCA000292505_00210" }, { "type": "cds", "sequence": "contig_1", "start": 117738, "stop": 118028, "strand": "+", "frame": 3, "gene": "ssb", "product": "Single-stranded DNA-binding protein", "db_xrefs": [ "COG:COG0629", "COG:L", "GO:0003697", "GO:0006260", "GO:0009295", "SO:0001217", "UniRef:UniRef50_P75542", "UniRef:UniRef90_P47337" ], "nt": "ATGCTTTTTGTAGAAGGAAGATTAACTACATATAAAATTACTAACAGTGAAAATAAAAACACCTATGCTTTACAAGTAACTGCTGATAAGATCTTTCATCCTGATGAAAAAACTACCAATGAAGAACCTATTAAATCAACTGTAGTTGATTCACCCTTTATGAATCCCAAAGCAAGTGTTACAGAAGCTGAGTTTGAACAAGCATTCCCCCATCAAGATGAAACTGATTTTAACAACATTACCCCTATATTTGAAAATGATGTCCAACTAGAGGAGGAAAGTGATGATTAA", "aa": "MLFVEGRLTTYKITNSENKNTYALQVTADKIFHPDEKTTNEEPIKSTVVDSPFMNPKASVTEAEFEQAFPHQDETDFNNITPIFENDVQLEEESDD", "aa_hexdigest": "63c4e090fab5027f158933171c84a084", "start_type": "ATG", "rbs_motif": null, "genes": [ "ssb" ], "truncated": "5-prime", "pseudogene": { "start": 117546, "stop": 118028, "observations": { "mutation": [ 117693 ], "stop": [ 117693 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47337", "query_cov": 1.0, "subject_cov": 0.6, "identity": 1.0, "score": 190.0, "evalue": 2.11e-60, "gene_start": 1, "gene_end": 96, "reference_start": 65, "reference_end": 160, "reference_sequence": "MNRVFLFGKLSFTPNRLQTKNGTLGATFSMECLDSSGFNNAKSFIRVTAWGKVASFIVAQNPGVMLFVEGRLTTYKITNSENKNTYALQVTADKIFHPDEKTTNEEPIKSTVVDSPFMNPKASVTEAEFEQAFPHQDETDFNNITPIFENDVQLEEESDD" }, "paralog": false, "identity": 0.99375, "score": 319.0, "evalue": 8.87e-114, "description": "Internal stop codon at 117693. Nonsense mutation around 117693.", "psc": { "uniref90_id": "UniRef90_P47337", "gene": "ssb", "product": "Single-stranded DNA-binding protein", "uniref50_id": "UniRef50_P75542", "cog_id": "COG0629", "cog_category": "L", "go_ids": [ "GO:0009295", "GO:0003697", "GO:0006260" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47337", "UniRef:UniRef50_P75542", "COG:COG0629", "COG:L", "GO:0009295", "GO:0003697", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_P75542", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75542" ], "product": "Single-stranded DNA-binding protein" } }, "id": "JPCNHPFPBM_250", "locus": "GCA000292505_00211" }, { "type": "cds", "sequence": "contig_1", "start": 118021, "stop": 118335, "strand": "+", "frame": 1, "gene": "rpsR", "product": "30S ribosomal protein S18", "db_xrefs": [ "COG:COG0238", "COG:J", "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "GO:0070181", "RefSeq:WP_009885648.1", "SO:0001217", "UniParc:UPI0000557C7D", "UniRef:UniRef100_P47338", "UniRef:UniRef50_P47338", "UniRef:UniRef90_P47338" ], "nt": "ATGATTAATAAAGAACAGGATTTAAACCAATTAGAAACCAACCAAGAACAGAGTGTTGAACAAAACCAAACTGATGAAAAGCGCAAGCCAAAACCAAACTTTAAAAGAGCAAAAAAATATTGTCGATTTTGCGCCATAGGTCAACTAAGGATTGATTTTATTGATGATTTGGAAGCAATCAAACGCTTTCTCAGTCCCTATGCAAAGATTAATCCTAGAAGAATTACAGGTAATTGCAACATGCACCAACGTCATGTAGCTAATGCTCTAAAACGAGCACGTTACCTAGCTTTAGTGCCATTTATTAAAGATTAA", "aa": "MINKEQDLNQLETNQEQSVEQNQTDEKRKPKPNFKRAKKYCRFCAIGQLRIDFIDDLEAIKRFLSPYAKINPRRITGNCNMHQRHVANALKRARYLALVPFIKD", "aa_hexdigest": "f3db5ce4b19d0a295cf2c340b55e09b3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000557C7D", "ncbi_nrp_id": "WP_009885648.1", "uniref100_id": "UniRef100_P47338", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000557C7D", "RefSeq:WP_009885648.1", "UniRef:UniRef100_P47338" ] }, "ips": { "uniref100_id": "UniRef100_P47338", "uniref90_id": "UniRef90_P47338" }, "psc": { "uniref90_id": "UniRef90_P47338", "gene": "rpsR", "product": "30S ribosomal protein S18", "uniref50_id": "UniRef50_P47338", "cog_id": "COG0238", "cog_category": "J", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "GO:0070181" ] }, "pscc": { "uniref50_id": "UniRef50_P47338", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47338" ], "product": "Small ribosomal subunit protein bS18" }, "genes": [ "rpsR" ], "id": "JPCNHPFPBM_251", "locus": "GCA000292505_00212" }, { "type": "cds", "sequence": "contig_1", "start": 118338, "stop": 118790, "strand": "+", "frame": 3, "gene": "rplI", "product": "50S ribosomal protein L9", "db_xrefs": [ "COG:COG0359", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "KEGG:K02939", "RefSeq:WP_009885649.1", "SO:0001217", "UniParc:UPI00001341F1", "UniRef:UniRef100_P47339", "UniRef:UniRef50_P75540", "UniRef:UniRef90_P47339" ], "nt": "ATGAAGATAATTTTGAAGCAAGATGTTGCTAAATTAGGCAAGCGGTTTGATGTTGTTGAAGTTAAAGATGGGTTTGCTATCCATTTTTTATTTCCCAAAAAACTAGCTGCACCTTTAACAAAGAAAGCAATTGCTAACCGTGATTTGTTTTTAAAACAACAACAAGAACAATACCAAAAAAATCGTGCCTTAGCTGAAAAATTGAAACTAGTAATTGAACAAACACCATTAACTTTTCAACTCAAACAACACGATGGCAAGCCATATGGTTCAATCATCACCAAACAAATAATTAATTTAGCAAAACAACAAAGACTTGATTTACAGCGCTTTATGTTTAAAGATAATGTGCGCTTACAGTTTGGTGAACACAAACTAATTTTGCACCTTTTTGAGGAGATAACTGCAACTTTAACTGTTATAGTGAACCCTGAAAATGGGACAACAAACTAG", "aa": "MKIILKQDVAKLGKRFDVVEVKDGFAIHFLFPKKLAAPLTKKAIANRDLFLKQQQEQYQKNRALAEKLKLVIEQTPLTFQLKQHDGKPYGSIITKQIINLAKQQRLDLQRFMFKDNVRLQFGEHKLILHLFEEITATLTVIVNPENGTTN", "aa_hexdigest": "bcfbc0281b4519f95d168d7380d55c03", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001341F1", "ncbi_nrp_id": "WP_009885649.1", "uniref100_id": "UniRef100_P47339", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001341F1", "RefSeq:WP_009885649.1", "UniRef:UniRef100_P47339" ] }, "ips": { "uniref100_id": "UniRef100_P47339", "uniref90_id": "UniRef90_P47339" }, "psc": { "uniref90_id": "UniRef90_P47339", "gene": "rplI", "product": "50S ribosomal protein L9", "uniref50_id": "UniRef50_P75540", "cog_id": "COG0359", "cog_category": "J", "kegg_orthology_id": "K02939", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P75540", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75540" ], "product": "Large ribosomal subunit protein bL9" }, "genes": [ "rplI" ], "id": "JPCNHPFPBM_252", "locus": "GCA000292505_00213" }, { "type": "cds", "sequence": "contig_1", "start": 118774, "stop": 119403, "strand": "+", "frame": 1, "gene": "dnaB", "product": "Replicative DNA helicase DnaB", "db_xrefs": [ "COG:COG0305", "COG:L", "EC:5.6.2.3", "GO:0003677", "GO:0003678", "GO:0003689", "GO:0005524", "GO:0005829", "GO:0006268", "GO:0006269", "GO:0009378", "GO:0016853", "GO:0016887", "GO:0036121", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990077", "GO:1990518", "KEGG:K02314", "SO:0001217", "UniRef:UniRef50_P47340", "UniRef:UniRef90_P47340" ], "nt": "ATGGGACAACAAACTAGTTTTAAATATGCAAATGATAGCAACATTGAACGTGCTGAAAGACGTTTGATGCAAGCAGTTGCTCAAAACAGTGAGGGCATTGATCTAATTTTCAATAAACTTGAACCAATTGATTTTTTTGCAACCCCTTTCAAACTCATTTTTCAAACTGCAAAAGAAAACTACCAATTAAATAACCCTATTATTGGTTCTGGTTTACTAGAAGCGGTTAAGTTTAAACTTGATGCTAATGATCAATCCACTAAAAGTGAACTTGAACTTTTATTCACAAAGATCTTATTAATCCGTTTACCACCTAACCAAACAGAGATTAAAACACTGGTTGATGTTGTTAAAAAAGCTTCTATTTTTCGCAGGTTACAACAGTTTGCTAAGCGTGTTTACAACGAGGAATTTAAGTTAAAAGAAGATCGTTTTGAAGGCTATTTACAAGCTATTCAAGATGATTTTGTCAAGATTATCCACAGTGCTTTTAGTAACATCTTTGCTTTTAGCTATGATGAGATTGCCAATCAAGAGGAAGCATTAATTAAAAAGGTTCACCGTGGGGAATTGATCATCAGTGGACTTTCAAGTGGATTTTTAAAATTAGATCAACTTACATCAGGTTGA", "aa": "MGQQTSFKYANDSNIERAERRLMQAVAQNSEGIDLIFNKLEPIDFFATPFKLIFQTAKENYQLNNPIIGSGLLEAVKFKLDANDQSTKSELELLFTKILLIRLPPNQTEIKTLVDVVKKASIFRRLQQFAKRVYNEEFKLKEDRFEGYLQAIQDDFVKIIHSAFSNIFAFSYDEIANQEEALIKKVHRGELIISGLSSGFLKLDQLTSG", "aa_hexdigest": "6bb9bdd9c63d19ac1119e7dbd7413338", "start_type": "ATG", "rbs_motif": null, "genes": [ "dnaB" ], "truncated": "3-prime", "pseudogene": { "start": 118774, "stop": 119706, "observations": { "mutation": [ 119401 ], "stop": [ 119401 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47340", "query_cov": 1.0, "subject_cov": 0.4465811965811966, "identity": 0.995, "score": 400.0, "evalue": 7.61e-137, "gene_start": 1, "gene_end": 209, "reference_start": 1, "reference_end": 209, "reference_sequence": "MGQQTSFKYANDSNIERAERRLMQAVAQNSEGIDLIFNKLEPIDFFATPFKLIFQTAKENYQLNNPIIGSGLLEAVKFKLDANDQSTKSELEILFTKILLIRLPPNQTEIKTLVDVVKKASIFRRLQQFAKRVYNEEFKLKEDRFEGYLQAIQDDFVKIIHSAFSNIFAFSYDEIANQEEALIKKVHRGELIISGLSSGFLKLDQLTSGWKPGELIVIAARPGRGKTALLINFMASAAKQIDPKTDVVLFFSLEMRNREIYQRHLMHESQTSYTLTNRQRINNVFEELMEASSRIKNLPIKLFDYSSLTLQEIRNQITEVSKTSNVRLVIIDYLQLVNALKNNYGLTRQQEVTMISQSLKAFAKEFNTPIIAAAQLSRRIEERKDSRPILSDLRESGSIEQDADMVLFIHRTNDDKKEQEEENTNLFEVELILEKNRNGPNGKVKLNFRSDTSSFISQYSPSFDDQYS" }, "paralog": false, "identity": 0.9935483870967742, "score": 594.0, "evalue": 7.06e-216, "description": "Internal stop codon at 119401. Nonsense mutation around 119401.", "psc": { "uniref90_id": "UniRef90_P47340", "gene": "dnaB", "product": "Replicative DNA helicase DnaB", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_P47340", "cog_id": "COG0305", "cog_category": "L", "kegg_orthology_id": "K02314", "go_ids": [ "GO:0005829", "GO:1990077", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0003678", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0006269", "GO:0006268" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47340", "EC:5.6.2.3", "UniRef:UniRef50_P47340", "COG:COG0305", "COG:L", "KEGG:K02314", "GO:0005829", "GO:1990077", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0003678", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0006269", "GO:0006268" ] }, "pscc": { "uniref50_id": "UniRef50_P47340", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47340" ], "product": "Replicative DNA helicase DnaB" } }, "id": "JPCNHPFPBM_253", "locus": "GCA000292505_00214" }, { "type": "cds", "sequence": "contig_1", "start": 119473, "stop": 120180, "strand": "+", "frame": 1, "gene": "dnaB", "product": "Replicative DNA helicase DnaB", "db_xrefs": [ "COG:COG0305", "COG:L", "EC:5.6.2.3", "GO:0003677", "GO:0003678", "GO:0003689", "GO:0005524", "GO:0005829", "GO:0006268", "GO:0006269", "GO:0009378", "GO:0016853", "GO:0016887", "GO:0036121", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990077", "GO:1990518", "KEGG:K02314", "SO:0001217", "UniRef:UniRef50_P47340", "UniRef:UniRef90_P47340" ], "nt": "ATGGCTAGTGCAGCTAAACAAATTGATCCTAAAACTGATGTGGTCCTCTTCTTTAGTTTAGAGATGCGTAACCGGGAAATTTACCAAAGGCACTTAATGCATGAAAGTCAAACTAGTTACACACTAACCAACCGACAAAGGATTAATAATGTCTTTGAAGAGTTAATGGAAGCATCTTCAAGGATCAAAAACTTACCTATTAAACTCTTTGATTACAGTAGTTTAACACTCCAAGAGATCAGAAACCAAATTACTGAAGTGAGTAAAACCAGTAATGTTAGGTTAGTAATTATTGACTATTTACAACTTGTTAATGCTTTAAAAAATAACTATGGTTTGACAAGACAACAAGAAGTGACAATGATCTCTCAATCACTTAAAGCATTTGCTAAGGAGTTTAATACCCCTATTATTGCTGCAGCTCAACTTTCTAGAAGGATTGAAGAAAGGAAAGATTCCAGACCAATTCTTTCTGATTTAAGAGAATCAGGTTCAATTGAACAGGATGCGGATATGGTTTTATTTATCCATAGAACTAATGATGATAAAAAAGAACAGGAAGAGGAGAACACAAACTTGTTTGAAGTGGAGCTTATCTTAGAAAAGAACAGAAATGGTCCCAATGGCAAAGTTAAACTAAACTTTCGCAGTGACACTTCTTCTTTTATTAGTCAATATTCCCCTAGTTTTGATGACCAATACAGTTAA", "aa": "MASAAKQIDPKTDVVLFFSLEMRNREIYQRHLMHESQTSYTLTNRQRINNVFEELMEASSRIKNLPIKLFDYSSLTLQEIRNQITEVSKTSNVRLVIIDYLQLVNALKNNYGLTRQQEVTMISQSLKAFAKEFNTPIIAAAQLSRRIEERKDSRPILSDLRESGSIEQDADMVLFIHRTNDDKKEQEEENTNLFEVELILEKNRNGPNGKVKLNFRSDTSSFISQYSPSFDDQYS", "aa_hexdigest": "b5651c07e719f73098f7488ccd5f6632", "start_type": "ATG", "rbs_motif": null, "genes": [ "dnaB" ], "truncated": "5-prime", "pseudogene": { "start": 119173, "stop": 120180, "observations": { "mutation": [ 119401 ], "stop": [ 119401 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47340", "query_cov": 1.0, "subject_cov": 0.5021367521367521, "identity": 1.0, "score": 441.0, "evalue": 8.85e-153, "gene_start": 1, "gene_end": 235, "reference_start": 234, "reference_end": 468, "reference_sequence": "MGQQTSFKYANDSNIERAERRLMQAVAQNSEGIDLIFNKLEPIDFFATPFKLIFQTAKENYQLNNPIIGSGLLEAVKFKLDANDQSTKSELEILFTKILLIRLPPNQTEIKTLVDVVKKASIFRRLQQFAKRVYNEEFKLKEDRFEGYLQAIQDDFVKIIHSAFSNIFAFSYDEIANQEEALIKKVHRGELIISGLSSGFLKLDQLTSGWKPGELIVIAARPGRGKTALLINFMASAAKQIDPKTDVVLFFSLEMRNREIYQRHLMHESQTSYTLTNRQRINNVFEELMEASSRIKNLPIKLFDYSSLTLQEIRNQITEVSKTSNVRLVIIDYLQLVNALKNNYGLTRQQEVTMISQSLKAFAKEFNTPIIAAAQLSRRIEERKDSRPILSDLRESGSIEQDADMVLFIHRTNDDKKEQEEENTNLFEVELILEKNRNGPNGKVKLNFRSDTSSFISQYSPSFDDQYS" }, "paralog": false, "identity": 0.9970149253731343, "score": 644.0, "evalue": 4.68e-235, "description": "Internal stop codon at 119401. Nonsense mutation around 119401.", "psc": { "uniref90_id": "UniRef90_P47340", "gene": "dnaB", "product": "Replicative DNA helicase DnaB", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_P47340", "cog_id": "COG0305", "cog_category": "L", "kegg_orthology_id": "K02314", "go_ids": [ "GO:0005829", "GO:1990077", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0003678", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0006269", "GO:0006268" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47340", "EC:5.6.2.3", "UniRef:UniRef50_P47340", "COG:COG0305", "COG:L", "KEGG:K02314", "GO:0005829", "GO:1990077", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003677", "GO:0003689", "GO:0003678", "GO:0036121", "GO:0061749", "GO:0009378", "GO:0016853", "GO:1990518", "GO:0006269", "GO:0006268" ] }, "pscc": { "uniref50_id": "UniRef50_P47340", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47340" ], "product": "Replicative DNA helicase DnaB" } }, "id": "JPCNHPFPBM_254", "locus": "GCA000292505_00215" }, { "type": "cds", "sequence": "contig_1", "start": 120184, "stop": 120531, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACTGAAGTTTTACAAACTACCTTTAATTACTACAGCATTTAGTTTTGTATTTTTAACTGCTTGTTCAACACCCCAATCTTCCTTTTTTCTCCCTGCACAAACAACTATTAGTACTTTAAAAATTAATGGTATGGAAAACAAAACAGGTTATTTTCTTGAAACGCAGCGTTCTCGTGGTAGTTACAATCCCACCGCTTCACTTACTATGATTAAGTTAGGAGATGAAAAAGAGTTTAAAAACGTAGATACAACCAAGCAAGATGAAGTTTTGTTTGCCAACATTTATGGTGGAATCTCTTCATTGCTTAACTTCAGAATTATCCAACCGATGTTAACCTACTGA", "aa": "MKLKFYKLPLITTAFSFVFLTACSTPQSSFFLPAQTTISTLKINGMENKTGYFLETQRSRGSYNPTASLTMIKLGDEKEFKNVDTTKQDEVLFANIYGGISSLLNFRIIQPMLTY", "aa_hexdigest": "4239874428945bbd1f667f1e9f076b0c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13014.951299999982, "isoelectric_point": 9.300869941711426 }, "id": "JPCNHPFPBM_255", "locus": "GCA000292505_00216" }, { "type": "cds", "sequence": "contig_1", "start": 120850, "stop": 120978, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAGGTATTGAAAATATTGAAGTTGAGTTTAGCTTTGTTAAAACTGGTTTTAATGGAAATGAGATTAAGTTTGAAGATTATGTAACAGATTCTTCCCCCACAACATCTTTATTAAAGCAAGTTTGA", "aa": "MIGIENIEVEFSFVKTGFNGNEIKFEDYVTDSSPTTSLLKQV", "aa_hexdigest": "8ed62026fe40d4ea6cd97c5ff932fbec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4728.241900000001, "isoelectric_point": 4.250954246520997 }, "id": "JPCNHPFPBM_256", "locus": "GCA000292505_00217" }, { "type": "cds", "sequence": "contig_1", "start": 121207, "stop": 121380, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTACTAACTGATTTGCAAAAGAAACAAGATCAAGTGAATCGGTTTGTTGGTTTTATTGGTCAAAACCACTTTACTTTAAGTGCTAACAGCATTAATGAAAAGCAGTTTAACGATGCTAGTACTGCTGATTTTTTTAAAGCAATCTTTCAAAAAGTGAGTATCAATGAATAA", "aa": "MLLTDLQKKQDQVNRFVGFIGQNHFTLSANSINEKQFNDASTADFFKAIFQKVSINE", "aa_hexdigest": "b143ce62319daa644f13bee0d843ce30", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6538.271699999999, "isoelectric_point": 6.541849708557129 }, "id": "JPCNHPFPBM_257", "locus": "GCA000292505_00218" }, { "type": "cds", "sequence": "contig_1", "start": 121564, "stop": 123195, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MG096", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47342", "UniRef:UniRef90_P47342" ], "nt": "TTGAAAACACAAAAAAATATCTTTAAGGTCAAGGCCTTACTACTTTCACTTTTTTCTGCTGCTGTTACCATCACTATTTTTGCTTTACCTATCTTTGCAAACAATGGTTCTAAAACTGATTTAGGTTTGCTTTCTAAAAATAGTGCTGATTTTTTAGGTAGTTCAAAAAGAAGTCTTGCTGGCTTTGATACTCCTTTTAGTCCAGATAACCTCCAGTATTTAGAAAAAGAAACTGACTATGATCAGAACTTTAAAAATTTTACTGAAAAGTTTAAAGATGAAAAAATAACTAACAATCAACTTGATATTGTTGATATCTATAACCTATTCAGTGGTTTTCACAAGAGTGTCAAAAACACAGTTGACTTAATGAGCCAACTGCAAAAGCAAGTTGAAGCTGCCAATGCTATCTTCCCAGTTGATGATGCTTTTGTTAAATTACCTAAAGTACCTACTGAATTATTTAAGTTAGTTGATGATAATGTCTTTCCTAAGTTAAATCCTAAGGGCTTAAATATCTCTGATAATATTGCTGCACTTTTTGAAAGATATAACCTTAAATCGATTGAACTTAAGAACTTTGACTTAGCTTTCTTGAGAAAAGCTGATGTAATTATCAAAGACAAGGTTCGATATAACTTTGAGATGCAAATGCAGTTTCAAACTGTTTATGTTGGTGGGGGTAATACCGTTATTAACTTAGACTTTACTTTAAAAGCCCAAACCGCTAACTTTGCTAACCTCCAGGATTTACAAAACAGTTTTGTTAAAGTTGGTAATGATCTTTCCACCCAACTCTTTTGGATCCCAACTGTTAATAAATTAACTGATAATGCAGGTAATGATCTTACCCATATTGCTAAAACTGTGATTGGTGAATCGTTTTTCCAAACCAATGTTAACTTAGCTAAATCAGTTATTGAATATGATAAGGTTCAACCATTGGTTAAACAAGCTTTTGAAGAGCGAGTTTTAACTCCTTTCAAAAAGGAAAGAGAAGCTGCTAAAAAAGCTTATGAAGAAGAACAACGTCGCTTGGAAGAGGAACGTAAGCGTCAACTAGAAGAGCTAAGGAGAAGAGAAGCTGAGGAGAAAAGAAAAGCTGAAGAAGCAAAACGAAATCAAGAAAAAGCACGCAGAGAAAGAGAAGCTTATGAAAAATCATTTAACTCCTTTAAAGACTTTAAATTTTACTGGTTAACTAAAGGTAAAGATGTTACTAAAAAAGCTGATTTAATTGATGCACTTAAAACCGCTATTGCTACACCAGCATACAGAAATAGAACATTCTCTTTATTAATCAAAGGTTTTGCTAGTGGAGTTGAACGTTATTTCAACGCTAACAAGAATGATAAAGAGTTGAAAAAACTTGCATTTGGTGAAAAAGGGATCCAATTCCCTAGAGCAGATGCTGGGATAGATGGTCTTTACATGAGTAATTTTTTGAGGCATGAATTGACAAGCAAAGCAAAATTTTCACTTAACCTAAAAGATATAAAAGTTGAAAATACGGTTGAAGATACTCAATTGTATTGGAAGGATAATGGCATTCATTTAAAGCAAGCCAATCCTTATAAATTCAATCTAAATATCAAGATCAAGTACAACGGCTGGTATAATGTCCACTGGTGA", "aa": "MKTQKNIFKVKALLLSLFSAAVTITIFALPIFANNGSKTDLGLLSKNSADFLGSSKRSLAGFDTPFSPDNLQYLEKETDYDQNFKNFTEKFKDEKITNNQLDIVDIYNLFSGFHKSVKNTVDLMSQLQKQVEAANAIFPVDDAFVKLPKVPTELFKLVDDNVFPKLNPKGLNISDNIAALFERYNLKSIELKNFDLAFLRKADVIIKDKVRYNFEMQMQFQTVYVGGGNTVINLDFTLKAQTANFANLQDLQNSFVKVGNDLSTQLFWIPTVNKLTDNAGNDLTHIAKTVIGESFFQTNVNLAKSVIEYDKVQPLVKQAFEERVLTPFKKEREAAKKAYEEEQRRLEEERKRQLEELRRREAEEKRKAEEAKRNQEKARREREAYEKSFNSFKDFKFYWLTKGKDVTKKADLIDALKTAIATPAYRNRTFSLLIKGFASGVERYFNANKNDKELKKLAFGEKGIQFPRADAGIDGLYMSNFLRHELTSKAKFSLNLKDIKVENTVEDTQLYWKDNGIHLKQANPYKFNLNIKIKYNGWYNVHW", "aa_hexdigest": "d9185d5476f335578de6939d7e6d8fa6", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47342", "query_cov": 0.998158379373849, "subject_cov": 0.8325652841781874, "identity": 0.982, "score": 947.0, "evalue": 0.0, "valid": true, "product": "Uncharacterized protein MG096", "uniref50_id": "UniRef50_P47342" }, "pscc": { "uniref50_id": "UniRef50_P47342", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47342" ], "product": "Uncharacterized protein MG096" }, "genes": [], "id": "JPCNHPFPBM_258", "locus": "GCA000292505_00219" }, { "type": "cds", "sequence": "contig_1", "start": 123247, "stop": 123516, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03072.19" ], "nt": "ATGAATTTAACATTTGTTGTCAACGGTGACCTATCAGAAATTGTTGATAAACATGATTACCCTGGTACTTTCTTTCAATTTACCGATAAAAATGAATTGCTATTTACCTTAGCAGTTAGAGAACAAATTAAGGTTGATAATAATCATTTTATGGGTCTGTTAAAAAGCCAAAACCTTCATGATCTTCAGCTTGCTTCTGGTGCTACAAAACCTCCTGTTGTTGATTTAGCTAGTTATTTCCACTTTGTATTATTGACTGAAAAATCTTAA", "aa": "MNLTFVVNGDLSEIVDKHDYPGTFFQFTDKNELLFTLAVREQIKVDNNHFMGLLKSQNLHDLQLASGATKPPVVDLASYFHFVLLTEKS", "aa_hexdigest": "c01381080050c9cde07b85f1b774866b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03072.19", "name": "DUF237", "length": 47, "aa_cov": 0.5168539325842697, "hmm_cov": 1.0, "evalue": 3.3870984387772967e-13, "score": 44.9623908996582, "start": 1, "stop": 46 } ], "seq_stats": { "molecular_weight": 10113.405299999991, "isoelectric_point": 5.354824256896973 }, "id": "JPCNHPFPBM_259", "locus": "GCA000292505_00220" }, { "type": "cds", "sequence": "contig_1", "start": 124044, "stop": 124271, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTAACTTTTCTAAAAAATCAAAACATTAAACCGCTGTTGGTATTTCTGGGTGTTCAAACTAAAAACTTTGTTGTTAAGAGTATTGGTAATGTTGATGGATTTGAGCATTTATCATATCCCCATCCCTCACCACTAAGTGGTAATTTGTTTCTAACAAACCCTAACGATCTGTTTAAAACAATTAACAATTGGTTGAAACAACATAACCAAAAAATAATTAACTGA", "aa": "MLTFLKNQNIKPLLVFLGVQTKNFVVKSIGNVDGFEHLSYPHPSPLSGNLFLTNPNDLFKTINNWLKQHNQKIIN", "aa_hexdigest": "39ebb89078017ab907b4aa6e583be638", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8589.877999999997, "isoelectric_point": 9.778645133972166 }, "id": "JPCNHPFPBM_260", "locus": "GCA000292505_00221" }, { "type": "cds", "sequence": "contig_1", "start": 124410, "stop": 125147, "strand": "+", "frame": 3, "gene": "gatC", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC", "db_xrefs": [ "COG:COG0721", "COG:J", "GO:0005886", "GO:0006424", "GO:0006450", "GO:0030956", "GO:0050567", "GO:0070681", "SO:0001217", "UniRef:UniRef50_P47344", "UniRef:UniRef90_P47344" ], "nt": "ATGGGAGAGATTAAAAATACTGCACCAACAAGCGATATTTCCACTTCAGGATTTATCTATTTTGCAGTTGTTTTTCTAATCATTATAGTCTATCTTTTTTTTAAAAACATTCTCTTTTTGTTCTTCTTTAAAAGATATCCTAAAAACACACCTAAAATTGGGGTTAGCAATATTACTACTATTGCTATGATTATTGCTGTTGCTGTATCTGTTGTTTTGGTCTTAATGGCTTTAGCAGGGGGGTTAACAGCAGCGCTGTTCCGTGGTTATCCTGGGTTCCGTGTTACCTTAGAGTTAATTCTAGTGAAGATTTCAGGACTTTTATTTGGTCCTATAATTGGTATTTTTTCAGCAGCTACCATTGACTTTTTAACTGTTATTTTTTCAGGTGGGGTGTTTAATATTGGTTATGTTTTAGGAGCAATCCTAACGGGAATGATTGCTGGAATTTTACGTGAAGTTTTAATTTCAACTTCTTTTTTAAATAACAAAACTTTAAGTGATTTTGCCTATCTTGTTTTATCAGTGGGGATGGTATTTGCCAGTTTTTTAGTAACCCAGTTTTTTGTTATCTCAGTTACCCAAAACTTATCAGCATTTCAAAGTAATGATCAAATTGTTTTACGTTTTAACGCCTCACCTTTAAATTTCAGTATCTCATTGCAAAGATATGTTCAAATTATTTTCTATTTTGCGATGGTTGTAATCATCACAATGGTAGTACTTTACTTTGTTTGA", "aa": "MGEIKNTAPTSDISTSGFIYFAVVFLIIIVYLFFKNILFLFFFKRYPKNTPKIGVSNITTIAMIIAVAVSVVLVLMALAGGLTAALFRGYPGFRVTLELILVKISGLLFGPIIGIFSAATIDFLTVIFSGGVFNIGYVLGAILTGMIAGILREVLISTSFLNNKTLSDFAYLVLSVGMVFASFLVTQFFVISVTQNLSAFQSNDQIVLRFNASPLNFSISLQRYVQIIFYFAMVVIITMVVLYFV", "aa_hexdigest": "5e54c5af3e0ccf8a3d223961fe32b9b2", "start_type": "ATG", "rbs_motif": null, "genes": [ "gatC" ], "truncated": "3-prime", "pseudogene": { "start": 124410, "stop": 125450, "observations": { "mutation": [ 125145, 125367 ], "stop": [ 125145, 125367 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47344", "query_cov": 1.0, "subject_cov": 0.5136268343815513, "identity": 1.0, "score": 427.0, "evalue": 7.38e-147, "gene_start": 1, "gene_end": 245, "reference_start": 1, "reference_end": 245, "reference_sequence": "MGEIKNTAPTSDISTSGFIYFAVVFLIIIVYLFFKNILFLFFFKRYPKNTPKIGVSNITTIAMIIAVAVSVVLVLMALAGGLTAALFRGYPGFRVTLELILVKISGLLFGPIIGIFSAATIDFLTVIFSGGVFNIGYVLGAILTGMIAGILREVLISTSFLNNKTLSDFAYLVLSVGMVFASFLVTQFFVISVTQNLSAFQSNDQIVLRFNASPLNFSISLQRYVQIIFYFAMVVIITMVVLYFVWIIKQKHFNYAYSKFFFRRYKHANHQFTLFVLTKENWFYLILNVITLATTSLLMINIAFIPIFDTQTTGQTYDFWLLVRLLFAPLIFLLDIIVIYPILLLLTPIMLKGFKTVASETQTKGIKKSFSDMQSLIMPNVISHKKQQLIRKEMQQLAKTIRIDLSDKEVDALVEEFKEITKSFNKVTKIDTTNVQPMYAPFEFSPTPLRKDKPVVDKHAKQLLNNCCEVKTGFVKV" }, "paralog": false, "identity": 0.9942196531791907, "score": 655.0, "evalue": 5.27e-239, "description": "Internal stop codon at 125145, 125367. Nonsense mutation around 125145, 125367.", "psc": { "uniref90_id": "UniRef90_P47344", "gene": "gatC", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC", "uniref50_id": "UniRef50_P47344", "cog_id": "COG0721", "cog_category": "J", "go_ids": [ "GO:0005886", "GO:0006424", "GO:0006450", "GO:0030956", "GO:0050567", "GO:0070681" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47344", "UniRef:UniRef50_P47344", "COG:COG0721", "COG:J", "GO:0005886", "GO:0006424", "GO:0006450", "GO:0030956", "GO:0050567", "GO:0070681" ] }, "pscc": { "uniref50_id": "UniRef50_P47344", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47344" ], "product": "Uncharacterized protein MG098" } }, "id": "JPCNHPFPBM_261", "locus": "GCA000292505_00222" }, { "type": "cds", "sequence": "contig_1", "start": 125235, "stop": 125369, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAACAAAAGAAAACTGGTTTTATCTAATTCTGAATGTAATTACATTAGCAACCACCAGTTTGCTAATGATTAACATTGCATTTATTCCTATCTTTGATACCCAAACAACTGGACAAACTTATGATTTTTGA", "aa": "MLTKENWFYLILNVITLATTSLLMINIAFIPIFDTQTTGQTYDF", "aa_hexdigest": "1ae888cae4e965623de8ebb0dcb8a1b2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5116.9391000000005, "isoelectric_point": 4.0500284194946286 }, "id": "JPCNHPFPBM_262", "locus": "GCA000292505_00223" }, { "type": "cds", "sequence": "contig_1", "start": 125385, "stop": 125843, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGCTCCTTTGATCTTCTTACTTGATATTATTGTTATCTATCCAATCTTGTTATTGTTAACCCCAATCATGTTAAAAGGGTTTAAAACAGTAGCATCAGAAACCCAAACAAAAGGAATCAAAAAGAGTTTTTCAGATATGCAAAGCTTAATTATGCCCAATGTTATTAGTCACAAAAAACAGCAGTTAATTAGAAAAGAGATGCAACAGTTAGCAAAAACAATCAGAATTGATTTATCAGACAAAGAAGTGGATGCACTTGTTGAAGAGTTTAAAGAGATCACAAAGAGTTTTAATAAGGTAACTAAAATTGATACCACTAATGTTCAACCGATGTACGCTCCATTTGAATTTAGTCCAACCCCACTAAGAAAAGATAAACCAGTAGTTGATAAACACGCTAAGCAACTACTTAATAACTGTTGTGAAGTTAAAACAGGTTTTGTAAAGGTATAG", "aa": "MFAPLIFLLDIIVIYPILLLLTPIMLKGFKTVASETQTKGIKKSFSDMQSLIMPNVISHKKQQLIRKEMQQLAKTIRIDLSDKEVDALVEEFKEITKSFNKVTKIDTTNVQPMYAPFEFSPTPLRKDKPVVDKHAKQLLNNCCEVKTGFVKV", "aa_hexdigest": "2f66afd55144866347b09c9b0c48e245", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17382.546599999998, "isoelectric_point": 9.416332817077638 }, "id": "JPCNHPFPBM_263", "locus": "GCA000292505_00224" }, { "type": "cds", "sequence": "contig_1", "start": 125846, "stop": 126655, "strand": "+", "frame": 2, "gene": "gatA", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA", "db_xrefs": [ "COG:COG0154", "COG:J", "EC:6.3.5.7", "GO:0005524", "GO:0006412", "GO:0006424", "GO:0030956", "GO:0050567", "SO:0001217", "UniRef:UniRef50_P47345", "UniRef:UniRef90_P47345" ], "nt": "GTGCGATCAAATATTTTAAGTCTCAGGGCGATACTTGATAAAAAACCTAGTGCCATTAACGATGTTTTAACATCAATTAATGCAAAGATAGAACTGAATAAATCAAGTAATTTTTTATTGAAAAATACTGTTGAAATTTATTCAAAAAAAATTAATAAAAGTGATGAAAAGATTCTGCTAAATAACATCCCTTATGTTTTGAAAGATAACATCGCTACTAAAGATATTGTCACCACTGGTGGTTCTTTGTTTTTAAAAAACTATCTTCCCCCTTTTTCAGCAACTGTGTTTGAACTGTTAGAAATGAATGGCGCGTTGCTTGTTGGTAAAGCTAATATGGATGAATTTGGCTTAGGTGGAACAGGTAGTTATTCTGCTTTTGGTGTTGTTCATCACCCTGAAAATTCCAGTTTAATTGCAGGTGGTTCTTCCTCAGGTTCAGCTTACGCAGTTGCTAAAGACATTGTTCCTTTTTCCATTGCAACTGACACTGGTGATTCGATTAGAAGACCTGCTAGTATCTGTAATGTTGTTGGCTTTAAACCAACTTATGGTTTGATATCACGTAATGGGGTATTTCCATATGCCCCAAGTATGGACCATGTGGGGATATTTGCTAAGTTTGTTAGTGATATTGCCATTGTTAGTGATGTTGTTATTAAACATGATAAAACTGATTTTTCTTCCCAAAAATCACCTGATGAAAACCAGTTTTTCAATGAGTTGGCCATTCCCTTTACAAGATCAATTCGCTTTGGTTATTTAAAACCACTAGAAAAACTGTTTAACAAACACCTCCAAAAAAAATGA", "aa": "MRSNILSLRAILDKKPSAINDVLTSINAKIELNKSSNFLLKNTVEIYSKKINKSDEKILLNNIPYVLKDNIATKDIVTTGGSLFLKNYLPPFSATVFELLEMNGALLVGKANMDEFGLGGTGSYSAFGVVHHPENSSLIAGGSSSGSAYAVAKDIVPFSIATDTGDSIRRPASICNVVGFKPTYGLISRNGVFPYAPSMDHVGIFAKFVSDIAIVSDVVIKHDKTDFSSQKSPDENQFFNELAIPFTRSIRFGYLKPLEKLFNKHLQKK", "aa_hexdigest": "cd92cb5d125a5d87cb7868e241d72642", "start_type": "GTG", "rbs_motif": null, "genes": [ "gatA" ], "truncated": "3-prime", "pseudogene": { "start": 125846, "stop": 126958, "observations": { "mutation": [ 126653 ], "stop": [ 126653 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47345", "query_cov": 1.0, "subject_cov": 0.5639412997903563, "identity": 1.0, "score": 517.0, "evalue": 7.86e-182, "gene_start": 1, "gene_end": 269, "reference_start": 1, "reference_end": 269, "reference_sequence": "MRSNILSLRAILDKKPSAINDVLTSINAKIELNKSSNFLLKNTVEIYSKKINKSDEKILLNNIPYVLKDNIATKDIVTTGGSLFLKNYLPPFSATVFELLEMNGALLVGKANMDEFGLGGTGSYSAFGVVHHPENSSLIAGGSSSGSAYAVAKDIVPFSIATDTGDSIRRPASICNVVGFKPTYGLISRNGVFPYAPSMDHVGIFAKFVSDIAIVSDVVIKHDKTDFSSQKSPDENQFFNELAIPFTRSIRFGYLKPLEKLFNKHLQKKWNNLKKTLEQKNYQLIPLDFDVELLKVIDSIYKIISYSEAVSCYSNLTGIVFGQKVFEPNSPSNFDQTITRNRDQFLGKQLKRRFVIGAFATDEKNFEKYFEKAQKIRRVLVDNFLNLFSDVDFVLSPSASCFASTIEDIQANKPYTNIIDDFLQLANFAGSPSITIPWLVQTKDQTIGLSISANCFEDKKLLQIAYWFEQLFDLNHD" }, "paralog": false, "identity": 0.9945945945945946, "score": 724.0, "evalue": 7.83e-266, "description": "Internal stop codon at 126653. Nonsense mutation around 126653.", "psc": { "uniref90_id": "UniRef90_P47345", "gene": "gatA", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA", "ec_ids": [ "6.3.5.7" ], "uniref50_id": "UniRef50_P47345", "cog_id": "COG0154", "cog_category": "J", "go_ids": [ "GO:0005524", "GO:0006412", "GO:0006424", "GO:0030956", "GO:0050567" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47345", "EC:6.3.5.7", "UniRef:UniRef50_P47345", "COG:COG0154", "COG:J", "GO:0005524", "GO:0006412", "GO:0006424", "GO:0030956", "GO:0050567" ] }, "pscc": { "uniref50_id": "UniRef50_P47345", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47345" ], "product": "Glutamyl-tRNA(Gln) amidotransferase subunit A" } }, "id": "JPCNHPFPBM_264", "locus": "GCA000292505_00225" }, { "type": "cds", "sequence": "contig_1", "start": 126695, "stop": 127246, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01425.27" ], "nt": "TTGATTCCACTTGATTTTGATGTGGAACTTCTCAAAGTAATTGATTCTATTTACAAAATAATTAGTTATAGCGAAGCAGTTAGTTGTTATAGTAATTTAACTGGCATTGTCTTTGGTCAAAAGGTGTTTGAACCTAATTCACCAAGTAATTTTGATCAAACTATTACCAGAAACAGAGATCAGTTTTTAGGTAAACAACTAAAAAGAAGATTTGTAATAGGGGCATTTGCAACTGATGAGAAGAATTTTGAAAAGTACTTTGAAAAAGCTCAGAAAATAAGAAGAATCTTAGTGGATAACTTTCTGAATCTCTTTAGTGATGTTGATTTTGTATTATCACCAAGCGCTTCTTGTTTTGCTAGTACCATTGAAGATATTCAAGCTAATAAGCCATATACAAACATTATTGATGACTTTTTACAATTAGCTAATTTTGCTGGTAGCCCTTCTATAACTATTCCTTGGTTAGTTCAAACAAAAGACCAAACAATTGGTTTAAGTATTAGTGCTAACTGTTTTGAAGATAAAAAACTCTTACAAATTGCTTATTGA", "aa": "MIPLDFDVELLKVIDSIYKIISYSEAVSCYSNLTGIVFGQKVFEPNSPSNFDQTITRNRDQFLGKQLKRRFVIGAFATDEKNFEKYFEKAQKIRRILVDNFLNLFSDVDFVLSPSASCFASTIEDIQANKPYTNIIDDFLQLANFAGSPSITIPWLVQTKDQTIGLSISANCFEDKKLLQIAY", "aa_hexdigest": "38d2f12708c7c023ed0dec1a780aa71d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01425.27", "name": "Amidase", "length": 180, "aa_cov": 0.9562841530054644, "hmm_cov": 0.9555555555555556, "evalue": 1.282539801501393e-30, "score": 101.9027099609375, "start": 5, "stop": 179 } ], "seq_stats": { "molecular_weight": 20821.549199999998, "isoelectric_point": 5.032376670837402 }, "id": "JPCNHPFPBM_265", "locus": "GCA000292505_00226" }, { "type": "cds", "sequence": "contig_1", "start": 127272, "stop": 128705, "strand": "+", "frame": 3, "gene": "gatB", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB", "db_xrefs": [ "COG:COG0064", "COG:J", "EC:6.3.5.-", "GO:0005524", "GO:0006412", "GO:0006424", "GO:0016884", "GO:0030956", "GO:0050566", "GO:0050567", "GO:0070681", "KEGG:K02434", "RefSeq:WP_009885658.1", "SO:0001217", "UniParc:UPI000012B155", "UniRef:UniRef100_P47346", "UniRef:UniRef50_P75533", "UniRef:UniRef90_P47346" ], "nt": "ATGATTAATTTTGAAGCGATTATTGGAATTGAAGTCCATGTAGTTTTAAATACTGCTAGCAAGATGTTTTCAAAAGCACCTAACCGCGTTGATAATCAAAAAATCAATCATTTTATTGACCCAATAGATTTAGGTTTACCAGGCACTTTGCCTCAAGTTAATGAGTTAGCAGTTTACAAGGCATTATTATTAGCTGATGCATTAAAAATGAAGACAGTAACAAATAAACTTGTTTTTGATCGAAAGCACTATTTTTATCAGGACTTACCTAAGGGTTTTCAAATCACCCAACAAAACTATCCTTTTGCTAAAAATGGTGTAGTTACCATTAATGTTGATGCTATTGAAAAACCAATTTATATTGACCGGTTTCATTTGGAGGAAGATACTGCCAAACAACACTTTAACCATGACCAAATTCTGCTTGATTTTAATAGGTGTGGTGCACCTTTAATTGAAGTTGTTACTCTTCCTGTTATCAACACTGCAAAAGAAGCGAAAGCCTACCTACAGAAGTTGAGACAAATTCTGATTGTTAACAATATCTCCAATGCCAAATTGGAAGATGGTTCAATGCGGAGCGATTGCAATGTTTCAGTACGTTTAAAAGGTCAAAGGCAACTAGGAACTAAAATTGAAATTAAAAACATCAACTCACTTAATAATGTTGAAAAAGCGATTGATCTTGAGATTAACCGCCAAGTTAAAGCACTTATTAATGGTGAAACCTTGAGTCAAGCAACTTTAAGCTTTGATGATAAAACCAACAACAATGTTTTTATGAGAAAAAAAGACAATACGATTGACTATAGGTACTTTATTGAACCTAACATCATGACTAGTAATATTGATGATTTATTATTAGAAAAACCTGTTGCTTTTCAGTTAGAACAGTTTCAAAAAGAACTAATTGATAGTAATGTCAATCCTCAATTAGTCCAATTAGTAGTTGATGATGCAACTATCTTTAGTGCTTTTCAAACTATTAATAGTGTGATTAAAAACCCCCAAGAAACCATCAGGTGGTTATGTATTGAACTAATAGGTCAACTCAATAAAACCAACAGTTCATTAACAGCTAATTTAATTCAAGATCTAATTACCCTAATTGAAATGCTAAAAGCAGCAAAGGTTAACCAAAAACAAGCTAAGCAGTTAATTACTTTAATGATTGAAACTAAAAAAGATCCAAAATCGCTAGCTAAGCTCCATAATTTAGAGCAAATTACTGATCCAAAAGAGTTACAAAAGATCATTAAAAAAATCTTTCAGGAAAATGAAAAAGAGATCCTGAAAAACATTGATAGAATTGAACGGATTCAAAAGTTAATTATTGGTCAAGTTATGCATAAGACCAATAATCGAGCAAACCCCCAACAAGTTTTTATAATTGTTGAAAATATGCTTCATGAAGTTCGGGAAAGAGATAGCTAA", "aa": "MINFEAIIGIEVHVVLNTASKMFSKAPNRVDNQKINHFIDPIDLGLPGTLPQVNELAVYKALLLADALKMKTVTNKLVFDRKHYFYQDLPKGFQITQQNYPFAKNGVVTINVDAIEKPIYIDRFHLEEDTAKQHFNHDQILLDFNRCGAPLIEVVTLPVINTAKEAKAYLQKLRQILIVNNISNAKLEDGSMRSDCNVSVRLKGQRQLGTKIEIKNINSLNNVEKAIDLEINRQVKALINGETLSQATLSFDDKTNNNVFMRKKDNTIDYRYFIEPNIMTSNIDDLLLEKPVAFQLEQFQKELIDSNVNPQLVQLVVDDATIFSAFQTINSVIKNPQETIRWLCIELIGQLNKTNSSLTANLIQDLITLIEMLKAAKVNQKQAKQLITLMIETKKDPKSLAKLHNLEQITDPKELQKIIKKIFQENEKEILKNIDRIERIQKLIIGQVMHKTNNRANPQQVFIIVENMLHEVRERDS", "aa_hexdigest": "3b7ee13d7bce7bfc8125a9424777e913", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012B155", "ncbi_nrp_id": "WP_009885658.1", "uniref100_id": "UniRef100_P47346", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012B155", "RefSeq:WP_009885658.1", "UniRef:UniRef100_P47346" ] }, "ips": { "uniref100_id": "UniRef100_P47346", "uniref90_id": "UniRef90_P47346" }, "psc": { "uniref90_id": "UniRef90_P47346", "gene": "gatB", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB", "ec_ids": [ "6.3.5.-" ], "uniref50_id": "UniRef50_P75533", "cog_id": "COG0064", "cog_category": "J", "kegg_orthology_id": "K02434", "go_ids": [ "GO:0005524", "GO:0006412", "GO:0006424", "GO:0016884", "GO:0030956", "GO:0050566", "GO:0050567", "GO:0070681" ] }, "pscc": { "uniref50_id": "UniRef50_P75533", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75533" ], "product": "Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B" }, "genes": [ "gatB" ], "id": "JPCNHPFPBM_266", "locus": "GCA000292505_00227" }, { "type": "cds", "sequence": "contig_1", "start": 128899, "stop": 129348, "strand": "+", "frame": 1, "gene": "mngR", "product": "putative HTH-type transcriptional regulator MG101", "db_xrefs": [ "COG:COG2188", "COG:K", "GO:0003677", "GO:0003700", "SO:0001217", "UniRef:UniRef50_P75532", "UniRef:UniRef90_P47347" ], "nt": "GTGTGTAAAGATGTAGGCTTTAGTTTTTTTCATAAAACCCAGGATAATCTGAAGGTGAAATATGCTAAACTTTCAACTTTGATTAAAAAGCTGTTATCACAGGATGATGCTAGTGTTTTTGCCAATATTGACAGTACTGTGCACCTTGATAAATTTAAGGGAATTGAAGCTAAGTTTTTTGATGAGAATAAAAAACACTTTTTAAACGTTTGTTTTTTTGCTAAAGATGATATTTTAAACATCCTTGATGAAAACAACCTCCAGCAACAGTTCTTCAGGGAGTTTGCTTACAATGGGATTGCTATTGAAAAACGTCATTGTGTTACCTCTGTTGACAAGGAAAGTGGGTGCTTGGTGATGTATGACATGTATTATGATGACAATGATAATTTTGTAGTTGCTAGCAAAAGTAACTTCCTTAACCCTGAATTGAAAGTAATCAATGCTTAA", "aa": "MCKDVGFSFFHKTQDNLKVKYAKLSTLIKKLLSQDDASVFANIDSTVHLDKFKGIEAKFFDENKKHFLNVCFFAKDDILNILDENNLQQQFFREFAYNGIAIEKRHCVTSVDKESGCLVMYDMYYDDNDNFVVASKSNFLNPELKVINA", "aa_hexdigest": "0a610266e88f07daa4cc4fff18420b8c", "start_type": "GTG", "rbs_motif": null, "genes": [ "mngR" ], "truncated": "5-prime", "pseudogene": { "start": 128680, "stop": 129348, "observations": { "mutation": [ 128758 ], "stop": [ 128758 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47347", "query_cov": 1.0, "subject_cov": 0.6711711711711712, "identity": 0.993, "score": 302.0, "evalue": 4.93e-103, "gene_start": 1, "gene_end": 149, "reference_start": 74, "reference_end": 222, "reference_sequence": "MKFGKEIAKKNQIIYRYIILKIQSFEWPANTRIFSERQLEIRFNSSRSQIRSVLATLLNKNIIRYTKNTPGYFVCKDVGFSFFHKTQDNLKVKYAKLSTLIKKLLSQDDASVFANIDSTVHLDKFKGIEAKFFDENKKHFLNVCFFAKDDILNILDENNLQQQFFREFAYNGIAIEKRHCVTSVDKESGCLVMYDMYYDDNDNFVVASKSNFLNPELKVINA" }, "paralog": false, "identity": 0.9954954954954955, "score": 442.0, "evalue": 1.69e-160, "description": "Internal stop codon at 128758. Nonsense mutation around 128758.", "psc": { "uniref90_id": "UniRef90_P47347", "gene": "MngR", "product": "Uncharacterized HTH-type transcriptional regulator MG101", "uniref50_id": "UniRef50_P75532", "cog_id": "COG2188", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0003700" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47347", "UniRef:UniRef50_P75532", "COG:COG2188", "COG:K", "GO:0003677", "GO:0003700" ] }, "pscc": { "uniref50_id": "UniRef50_P75532", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75532" ], "product": "Uncharacterized HTH-type transcriptional regulator MG101 homolog" } }, "id": "JPCNHPFPBM_267", "locus": "GCA000292505_00228" }, { "type": "cds", "sequence": "contig_1", "start": 129341, "stop": 130288, "strand": "+", "frame": 2, "gene": "trxB", "product": "thioredoxin-disulfide reductase", "db_xrefs": [ "COG:COG0492", "COG:O", "EC:1.8.1.9", "GO:0004791", "GO:0005737", "GO:0019430", "GO:0045454", "GO:0071949", "RefSeq:WP_009885660.1", "SO:0001217", "UniParc:UPI00001376C6", "UniRef:UniRef100_P47348", "UniRef:UniRef50_P47348", "UniRef:UniRef90_P47348" ], "nt": "ATGCTTAAAGTGAATGCTGATTTTTTAACTAAAGATCAAGTTATCTATGATTTAGTGATAGTAGGTGCTGGCCCTGCTGGGATTGCTAGTGCCATTTATGGTAAACGTGCTAACTTAAATTTAGCAATTATTGAAGGAAACACTCCAGGAGGGAAGATAGTAAAAACTAACATTGTGGAAAACTATCCTGGTTTTAAAACCATAACTGGTCCTGAATTAGGTCTTGAGATGTACAACCACTTGTTAGCATTTGAACCAGTTGTTTTTTATAACAACTTAATCAAAATTGATCATCTTAACGATACATTCATCTTGTATTTAGATAACAAAACGACAGTTTTTAGCAAAACTGTTATCTATGCAACAGGGATGGAAGAGAGAAAACTTGGCATTGAAAAGGAAGATTATTTTTATGGTAAAGGGATTAGTTATTGTGCTATTTGTGATGCGGCTCTTTACAAAGGTAAAACAGTTGGTGTTGTAGGAGGAGGTAATTCTGCAATACAGGAAGCAATTTATCTTTCAAGTATTGCTAAAACAGTTCACCTTATTCACAGACGTGAAGTGTTTAGAAGTGATGCATTACTAGTTGAAAAATTAAAAAAAATTAGTAATGTAGTTTTTCATTTAAATGCTACTGTAAAACAGTTAATAGGTCAAGAAAAGCTCCAAACTGTTAAATTGGCAAGCACAGTTGATAAATCAGAAAGTGAAATTGCAATTGATTGTCTCTTTCCTTACATAGGCTTTGAAAGTAATAACAAGCCAGTTTTAGATCTTAAGCTTAATTTAGATCAAAATGGTTTTATTTTAGGAGATGAAAATATGCAAACTAACATTAAGGGTTTTTATGTTGCTGGGGATTGTAGAAGTAAATCATTCCGGCAAATTGCCACTGCAATTAGTGATGGGGTAACAGCTGTTTTAAAGGTTAGGGATGACATTTAG", "aa": "MLKVNADFLTKDQVIYDLVIVGAGPAGIASAIYGKRANLNLAIIEGNTPGGKIVKTNIVENYPGFKTITGPELGLEMYNHLLAFEPVVFYNNLIKIDHLNDTFILYLDNKTTVFSKTVIYATGMEERKLGIEKEDYFYGKGISYCAICDAALYKGKTVGVVGGGNSAIQEAIYLSSIAKTVHLIHRREVFRSDALLVEKLKKISNVVFHLNATVKQLIGQEKLQTVKLASTVDKSESEIAIDCLFPYIGFESNNKPVLDLKLNLDQNGFILGDENMQTNIKGFYVAGDCRSKSFRQIATAISDGVTAVLKVRDDI", "aa_hexdigest": "c62ad9a7e9d36b17e652e480cf0c7955", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001376C6", "ncbi_nrp_id": "WP_009885660.1", "uniref100_id": "UniRef100_P47348", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001376C6", "RefSeq:WP_009885660.1", "UniRef:UniRef100_P47348" ] }, "ips": { "uniref100_id": "UniRef100_P47348", "uniref90_id": "UniRef90_P47348" }, "psc": { "uniref90_id": "UniRef90_P47348", "gene": "trxB", "product": "thioredoxin-disulfide reductase", "ec_ids": [ "1.8.1.9" ], "uniref50_id": "UniRef50_P47348", "cog_id": "COG0492", "cog_category": "O", "go_ids": [ "GO:0004791", "GO:0005737", "GO:0019430", "GO:0045454", "GO:0071949" ] }, "pscc": { "uniref50_id": "UniRef50_P47348", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47348" ], "product": "Thioredoxin reductase" }, "genes": [ "trxB" ], "id": "JPCNHPFPBM_268", "locus": "GCA000292505_00229" }, { "type": "cds", "sequence": "contig_1", "start": 130410, "stop": 131015, "strand": "+", "frame": 3, "gene": "whiA", "product": "DNA-binding protein WhiA", "db_xrefs": [ "COG:COG1481", "COG:K", "GO:0003677", "GO:0043937", "GO:0051301", "SO:0001217", "UniRef:UniRef50_P47349", "UniRef:UniRef90_P47349" ], "nt": "GTGCAATCTGAAGCATTAAAGGAACAATTTGTTCAAAATCTTAAGTTTGACTTTAAGACAAAAGCTAGTAAGAAATACTTTCTTTTTGAGTTTAATGCAGATATTAACGTAATTAACACTCTTTTAAAACTTGATGTGACAACTAGTGAATTGGTAGTTAAACAAGTTTATCTCATTGCTGCTTTTTTAAGTGGAGGTAGTGTTAGTGATTTAATAAACTCCAATAACTTTCACTTGCAAATCAGTTCCAACAATGAGTTTCAAATTCAACAACTTTTAAAATTGTTTAGTTTTTTTAAAAAAACAGTTAAACAAAACCAGTTAGTTGTTTATCTTAAAAGTTATGAAAAGATCTGTAATTTTTTAAAACTGATTCAAGCCTTTGATGGTTATCTTGCTTTTGAAAATAAGCAACTAGAGAAAAGTTTTACTTTAAACCAGTTAAGAAAAAGTAATTTGGAAGTTGCTAACTTAATGAAGACAATCAGATCTAATAATCAAACTAATCAACTCCAACTAAAATCATTTATTAAAAGTAGTAGTTTTGCAAAAAGACCGCTTAATTTTCAGCGTTATTGCTTAATTAAAAGTGATCATCCTGATTGA", "aa": "MQSEALKEQFVQNLKFDFKTKASKKYFLFEFNADINVINTLLKLDVTTSELVVKQVYLIAAFLSGGSVSDLINSNNFHLQISSNNEFQIQQLLKLFSFFKKTVKQNQLVVYLKSYEKICNFLKLIQAFDGYLAFENKQLEKSFTLNQLRKSNLEVANLMKTIRSNNQTNQLQLKSFIKSSSFAKRPLNFQRYCLIKSDHPD", "aa_hexdigest": "30fc90082c1df06ba3d8cd002482d3ca", "start_type": "GTG", "rbs_motif": null, "genes": [ "whiA" ], "truncated": "both", "pseudogene": { "start": 130278, "stop": 131120, "observations": { "mutation": [ 130338, 130398, 131013 ], "stop": [ 130338, 130398, 131013 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47349", "query_cov": 1.0, "subject_cov": 0.7178571428571429, "identity": 0.995, "score": 376.0, "evalue": 1.77e-130, "gene_start": 1, "gene_end": 201, "reference_start": 45, "reference_end": 245, "reference_sequence": "MTFSTQIKAELVQNKLIDKHWNVFLAGFFQNNLKLLYNRNWSFKVQSEALKEQFVQNLKFDFKTKASKKYFLFEFNADINVINTLLKLDVTTSELVVKQVYLIAAFLSGGSVSDLINSNNFHLQISSNNEFQIQQLLKLFSFFKKTVKQNQLVVYLKSYEKICNFLKLIQAFDGYLAFENKQLEKSFTLNQLRKSNLEVANLMKTIRSNNQTNQLQLKSFIKSSSFAKRPLNFQRYCLIKSDHPDWSLEQIANFFFTKYNIKISRSGIQHFSVNLKKLCQ" }, "paralog": false, "identity": 0.9892857142857143, "score": 536.0, "evalue": 7.38e-196, "description": "Internal stop codon at 130338, 130398, 131013. Nonsense mutation around 130338, 130398, 131013.", "psc": { "uniref90_id": "UniRef90_P47349", "gene": "whiA", "product": "DNA-binding protein WhiA", "uniref50_id": "UniRef50_P47349", "cog_id": "COG1481", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0043937", "GO:0051301" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47349", "UniRef:UniRef50_P47349", "COG:COG1481", "COG:K", "GO:0003677", "GO:0043937", "GO:0051301" ] }, "pscc": { "uniref50_id": "UniRef50_P47349", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47349" ], "product": "Probable cell division protein WhiA" } }, "id": "JPCNHPFPBM_269", "locus": "GCA000292505_00230" }, { "type": "cds", "sequence": "contig_1", "start": 131142, "stop": 131375, "strand": "+", "frame": 3, "gene": "secG", "product": "preprotein translocase subunit SecG", "db_xrefs": [ "GO:0005886", "GO:0009306", "GO:0015136", "GO:0015450", "GO:0015739", "GO:0016020", "RefSeq:WP_010869333.1", "SO:0001217", "UniParc:UPI00001357BA", "UniRef:UniRef100_P58061", "UniRef:UniRef50_P58061", "UniRef:UniRef90_P58061" ], "nt": "ATGCATCCAATCCAAATAGTAATGTTCATTATGGCTGTTATCTGTTTAATTATTGGACTTTTGCTTTCTAACCATGGTTCTACTGGAGGATTAGCTTCTCTATCAGGTCAGGACTTGGAGATCTTTCGTAAAACCAAAGATAGGGGTTTTGTAAAGATCTTACAGATTATCATGTTTATCTTAGTAGTTTTATTTTTAATTCTTGGGTTGATATTTAGTTTTGCACCAAGATAA", "aa": "MHPIQIVMFIMAVICLIIGLLLSNHGSTGGLASLSGQDLEIFRKTKDRGFVKILQIIMFILVVLFLILGLIFSFAPR", "aa_hexdigest": "ae937ae76cf05dd412b9491e9bfeb245", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001357BA", "ncbi_nrp_id": "WP_010869333.1", "uniref100_id": "UniRef100_P58061", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001357BA", "RefSeq:WP_010869333.1", "UniRef:UniRef100_P58061" ] }, "ips": { "uniref100_id": "UniRef100_P58061", "uniref90_id": "UniRef90_P58061" }, "psc": { "uniref90_id": "UniRef90_P58061", "gene": "secG", "product": "preprotein translocase subunit SecG", "uniref50_id": "UniRef50_P58061", "go_ids": [ "GO:0005886", "GO:0009306", "GO:0015136", "GO:0015450", "GO:0015739", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P58061", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P58061" ], "product": "Probable protein-export membrane protein SecG" }, "genes": [ "secG" ], "id": "JPCNHPFPBM_270", "locus": "GCA000292505_00231" }, { "type": "cds", "sequence": "contig_1", "start": 131378, "stop": 131539, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGTTTTAACTGAACTTCAAAAGCAGATATTTACCATTGTCAAAAAGGAAAATGGTAAACCTATTCCCCCTGGAATAGTGGTAAGAATGATGGAAAATAGTCCTAATTTTCCAGGTAAACACCTCATCTATCGGGCCATTGATGATCTGCTTGATTGA", "aa": "MKVLTELQKQIFTIVKKENGKPIPPGIVVRMMENSPNFPGKHLIYRAIDDLLD", "aa_hexdigest": "5a38b02da334b0cefc910e0643653a80", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6077.189500000001, "isoelectric_point": 9.22350788116455 }, "id": "JPCNHPFPBM_271", "locus": "GCA000292505_00232" }, { "type": "cds", "sequence": "contig_1", "start": 131672, "stop": 132958, "strand": "+", "frame": 2, "gene": "rnr", "product": "ribonuclease R", "db_xrefs": [ "COG:COG0557", "COG:K", "EC:3.1.13.1", "GO:0003723", "GO:0004518", "GO:0005829", "GO:0006402", "GO:0008859", "KEGG:K12573", "SO:0001217", "UniRef:UniRef50_P47350", "UniRef:UniRef90_P47350" ], "nt": "TTGGATGATGATAAAACTGTGTATTATGTCCATTACTCTAATTTAACTGGAGCTTTAGATGGGGATCTTGTGGAGTTTTGTAAATTAGATAAGCCACAATTTGGTGATAAGTTTGATGCTGCAGTTATTACTATTCTAAAAAGAGCAAGAATCTTGTATGCAGGTAATTTTTTAGTAGATCAAAATGAGTTTGCCTTGGAATACAAAATTGTTGCTGATAACCCTAGATTTTATTTAACTACGATTGTAAATCCTGATTCTATCCCAAATAACTTAGCATCTAACACCAAGATAGCTTTTCAAATTGATGAGTATGATCCTGATAACAACTTATGTAAGGTTTCTGTACAACAAGTTTTGGGTAACAATGATGATCCGCTAATTAATATAAAAGCAATCATGTTGGACAATTCCATTGTCTTTGAAACTAACGATGTAGTTGAACAGCATGCTAACAAGTTAAGTTTTGATACTGAAGAACAACATAAAGCTTACCGTCAGGATTTAACTGATTTAGCTTTTGTGACTGTTGATCCTACAACATCAAAAGACCTTGATGATGCTATTTATGTCAAAACAATACCAACAGGTTTTGTGCTTTATGTAGCTATTGCTGATGTTGCACACTATGTTAATAGAAATAGTGAAATAGACATTGAAGCAAAACACAAAACAAGCTCAATCTATCTACCTGGTCATTATGTTGTGCCCATGCTACCTGAGCAATTGTCAAATCAGCTCTGTTCTTTAAATCCAGCACAAAAACGTTATGTTGTTGTTTGTGAGATTAGTTTTGATAATCAGGGAAGGATTAAAACAAACAAGCTTTACCCAGCAACAATTATCTCCAAAAATCGCTTTAGCTATGATCAGGTTAACCAGTGGTTAAATAATAAATCAGAATTAAACTGTGATGAAACAGTTATCAACAGCTTAAAAGCAGCATTTACACTAAGTGATCTAATTCAAAAGCAACGTCAAAAACGCGGTACAATTGATCTTTCACACAAAGAAACTGAGATAGTTGTTGATGAACATTATTTTCCCATTAAGATAAATTTTTTGGTTCACGATAAAGCTGAAACCATGATTGAAAATCTCATGGTAGTGGCCAATGAGACAGTTGCTTGGGTGTTAACTAACAACAAAATTGCTTTACCATACAGAGTTCACCCAAGACCAAGCAAAAAGAAGTTACAAAGTTTGATTGAAACAGTTGGTGAGTTGAACATAACTAAACCCCAATTTAACTTAGATACTGTCACTTCAAGCCAAATAGCTAGCTGA", "aa": "MDDDKTVYYVHYSNLTGALDGDLVEFCKLDKPQFGDKFDAAVITILKRARILYAGNFLVDQNEFALEYKIVADNPRFYLTTIVNPDSIPNNLASNTKIAFQIDEYDPDNNLCKVSVQQVLGNNDDPLINIKAIMLDNSIVFETNDVVEQHANKLSFDTEEQHKAYRQDLTDLAFVTVDPTTSKDLDDAIYVKTIPTGFVLYVAIADVAHYVNRNSEIDIEAKHKTSSIYLPGHYVVPMLPEQLSNQLCSLNPAQKRYVVVCEISFDNQGRIKTNKLYPATIISKNRFSYDQVNQWLNNKSELNCDETVINSLKAAFTLSDLIQKQRQKRGTIDLSHKETEIVVDEHYFPIKINFLVHDKAETMIENLMVVANETVAWVLTNNKIALPYRVHPRPSKKKLQSLIETVGELNITKPQFNLDTVTSSQIAS", "aa_hexdigest": "2162a68df4fc44bb2d718facb37f8c90", "start_type": "TTG", "rbs_motif": null, "genes": [ "rnr" ], "truncated": "both", "pseudogene": { "start": 131378, "stop": 133261, "observations": { "mutation": [ 131537, 132956, 133139 ], "stop": [ 131537, 132956, 133139 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47350", "query_cov": 1.0, "subject_cov": 0.5729585006693441, "identity": 0.988, "score": 836.0, "evalue": 5.91e-301, "gene_start": 1, "gene_end": 428, "reference_start": 121, "reference_end": 548, "reference_sequence": "MGHHHHHHHHHHSSGHIDDDDKMKVLTELQKQIFTIVKKENGKPIPPGIVVRMMENSPNFPGKHLIYRAIDDLLDWAILRKAGGVTNQLLVNYEPAEPLLDKKLQGILTLGNKNSGFIRSLDDDKTVYYVHYSNLTGALDGDLVEFCKLDKPQFGDKFDAAVITILKRARILYAGNFLVDQNEFALEYKIVADNPRFYLTMIVNPDSIPNNLASNTKIAFQIDEYDPDNNLCKVSVQQVLGNNDDPLINIKAIMLDNSIVFETNDVVEQHANKLSFDTEEQHKAYRQDLTDLAFVTVDPTTSKDLADAIYVKTIPTGFVLYVAIADVAHYVNRNSEIDIEAKHKTSSIYLPGHYVVPMLPEQLSNQLCSLNPAQKRYVVVCEISFDNQGRIKTNKLYPATIISKNRFSYDQVNKWLNNKSELNCDETVINSLKAAFTLSDLIQAQRQKRGTIDLSHKETEIVVDEHYFPIKINFLVHDKAETMIENLMVVANETVAWVLTNNKIALPYRVHPRPSKKKLQSLIETVGELNITKPQFNLDTVTSSQIASWLNENKDNPSYEIFVILLLRTLGKAFYSVNPLMHFSIGSNHYTHFTSPIRRYIDLTIHRLLWMHLFTPDQFTDNERDQLKQELEKIADTVNDTEIKIINCERNANDYLTTLLLSKQIGKTFSGFISAITSFGIFMRMDENNFDGLIKITTIPDDFFIFEKEKMVLKGRKTNKVYKIGDRLEAKLSEIDFIQKRAILTLI" }, "paralog": false, "identity": 0.988835725677831, "score": 1232.0, "evalue": 0.0, "description": "Internal stop codon at 131537, 132956, 133139. Nonsense mutation around 131537, 132956, 133139.", "psc": { "uniref90_id": "UniRef90_P47350", "gene": "rnr", "product": "ribonuclease R", "ec_ids": [ "3.1.13.1" ], "uniref50_id": "UniRef50_P47350", "cog_id": "COG0557", "cog_category": "K", "kegg_orthology_id": "K12573", "go_ids": [ "GO:0003723", "GO:0004518", "GO:0005829", "GO:0006402", "GO:0008859" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47350", "EC:3.1.13.1", "UniRef:UniRef50_P47350", "COG:COG0557", "COG:K", "KEGG:K12573", "GO:0003723", "GO:0004518", "GO:0005829", "GO:0006402", "GO:0008859" ] }, "pscc": { "uniref50_id": "UniRef50_P47350", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47350" ], "product": "Ribonuclease R" } }, "id": "JPCNHPFPBM_272", "locus": "GCA000292505_00233" }, { "type": "cds", "sequence": "contig_1", "start": 133142, "stop": 133555, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00575.29" ], "nt": "ATGCATCTTTTTACTCCCGATCAATTCACTGATAATGAAAGAGATCAACTCAAACAAGAGTTGGAAAAAATTGCTGATACAGTTAATGATACAGAGATTAAAATTATCAATTGTGAAAGAAATGCCAATGATTATCTAACAACGCTGTTATTATCAAAACAAATTGGCAAAACCTTCAGCGGATTTATTTCAGCAATTACTAGCTTTGGAATTTTTATGAGAATGGATGAAAATAACTTTGATGGGTTAATCAAAATTACAACTATCCCTGATGATTTCTTTATTTTTGAAAAGGAAAAAATGGTATTGAAAGGAAGAAAAACTAATAAGGTTTATAAAATTGGCGATCGTTTGGAAGCTAAACTAAGTGAGATTGATTTCATCCAAAAACGTGCTATTTTAACACTCATATAA", "aa": "MHLFTPDQFTDNERDQLKQELEKIADTVNDTEIKIINCERNANDYLTTLLLSKQIGKTFSGFISAITSFGIFMRMDENNFDGLIKITTIPDDFFIFEKEKMVLKGRKTNKVYKIGDRLEAKLSEIDFIQKRAILTLI", "aa_hexdigest": "9bf5b7c9f3b55addc28ef91f1e12d655", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00575.29", "name": "S1", "length": 82, "aa_cov": 0.5985401459854015, "hmm_cov": 0.8536585365853658, "evalue": 5.151817981440019e-12, "score": 41.166011810302734, "start": 54, "stop": 135 } ], "seq_stats": { "molecular_weight": 15918.203699999987, "isoelectric_point": 5.487600135803221 }, "id": "JPCNHPFPBM_273", "locus": "GCA000292505_00234" }, { "type": "cds", "sequence": "contig_1", "start": 133563, "stop": 133679, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGTTAATATCCTGCTTTTTATTACCCTTATTTTTTTACTTTTACTGTTTGTTTTTTTAATTGCTTTTGCATTTCTAAACAAACGGGTTAGAAATTATGTTGTACGTACTTGA", "aa": "MVVNILLFITLIFLLLLFVFLIAFAFLNKRVRNYVVRT", "aa_hexdigest": "e7bad74b7219d04b6fd7a3731452fcfd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4544.662900000002, "isoelectric_point": 11.722947120666507 }, "id": "JPCNHPFPBM_274", "locus": "GCA000292505_00235" }, { "type": "cds", "sequence": "contig_1", "start": 133914, "stop": 134165, "strand": "+", "frame": 3, "gene": "cdaM", "product": "diadenylate cyclase CdaM", "db_xrefs": [ "COG:COG1624", "COG:T", "EC:2.7.7.85", "GO:0004016", "GO:0005524", "GO:0005886", "GO:0006171", "GO:0106408", "KEGG:K18672", "SO:0001217", "UniRef:UniRef50_P47351", "UniRef:UniRef90_P47351" ], "nt": "GTGATTGTTAGAGATTACAAAATTATTAGTGTCTCTTCTTACTTTCCAATGACAAGACAATTAATTGATGTTTCATATGGTTCAAGACACAGAAGTGCCTTGGGATTAAGTGAAAAGTCTGATGCAGTTGTTTTTATTGTTAGTGAAACAACTGGTAAAATATCAGTTGCTTTAAAAGGGGTTATTAAAACCTTGTCTTCCAACTCAGATAGGTTGCAAGATGAAATTATCCATTACTTGTCTTCAAAGTAG", "aa": "MIVRDYKIISVSSYFPMTRQLIDVSYGSRHRSALGLSEKSDAVVFIVSETTGKISVALKGVIKTLSSNSDRLQDEIIHYLSSK", "aa_hexdigest": "5729fce41ac549b731dc69104e4ea5cb", "start_type": "GTG", "rbs_motif": null, "genes": [ "cdaM" ], "truncated": "5-prime", "pseudogene": { "start": 133620, "stop": 134165, "observations": { "mutation": [ 133677 ], "stop": [ 133677 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47351", "query_cov": 0.9879518072289156, "subject_cov": 0.41, "identity": 1.0, "score": 155.0, "evalue": 2.94e-46, "gene_start": 2, "gene_end": 83, "reference_start": 119, "reference_end": 200, "reference_sequence": "MVVNILLFITLIFLLLLFVFLIAFAFLNKRVRNYVVRTWTSVFSKSKQNLDKKNFFDNLTSTLLRLSVDKIGAIIAIEKRDSLDPYINIGYRVSSDFSPELLVTIFYNKSSPLHDGAVIVRDYKIISVSSYFPMTRQLIDVSYGSRHRSALGLSEKSDAVVFIVSETTGKISVALKGVIKTLSSNSDRLQDEIIHYLSSK" }, "paralog": false, "identity": 0.994475138121547, "score": 343.0, "evalue": 4.05e-123, "description": "Internal stop codon at 133677. Nonsense mutation around 133677.", "psc": { "uniref90_id": "UniRef90_P47351", "gene": "cdaM", "product": "diadenylate cyclase CdaM", "ec_ids": [ "2.7.7.85" ], "uniref50_id": "UniRef50_P47351", "cog_id": "COG1624", "cog_category": "T", "kegg_orthology_id": "K18672", "go_ids": [ "GO:0005886", "GO:0004016", "GO:0005524", "GO:0106408", "GO:0006171" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47351", "EC:2.7.7.85", "UniRef:UniRef50_P47351", "COG:COG1624", "COG:T", "KEGG:K18672", "GO:0005886", "GO:0004016", "GO:0005524", "GO:0106408", "GO:0006171" ] }, "pscc": { "uniref50_id": "UniRef50_P47351", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47351" ], "product": "Diadenylate cyclase" } }, "id": "JPCNHPFPBM_275", "locus": "GCA000292505_00236" }, { "type": "cds", "sequence": "contig_1", "start": 134509, "stop": 134721, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01327.26" ], "nt": "ATGACTTTTCAACCAACTAAAACTTGGCTTGTTTTTGATGATAATGCTTTGATTAACAAGCCAACTGAAGCTGTTAATTTTCCAATAGATGAGCAAATTGAAACCTGTATTAAAAAGATGATTGCATATGTTGATGCTTCATATGATGGTAAAGCACAAGAATATGACATTATTCCAGGAATTGGGATAGCTGCTAACCAGATTGGCTATTGA", "aa": "MTFQPTKTWLVFDDNALINKPTEAVNFPIDEQIETCIKKMIAYVDASYDGKAQEYDIIPGIGIAANQIGY", "aa_hexdigest": "f68adf1a996fd0d66b84e5bf1b67bbdd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01327.26", "name": "Pep_deformylase", "length": 61, "aa_cov": 0.8571428571428571, "hmm_cov": 0.8032786885245902, "evalue": 2.075215984223976e-07, "score": 25.789291381835938, "start": 11, "stop": 70 } ], "seq_stats": { "molecular_weight": 7842.8357000000005, "isoelectric_point": 4.2393590927124025 }, "id": "JPCNHPFPBM_276", "locus": "GCA000292505_00237" }, { "type": "cds", "sequence": "contig_1", "start": 134762, "stop": 135331, "strand": "+", "frame": 2, "gene": "gmk", "product": "guanylate kinase", "db_xrefs": [ "COG:COG0194", "COG:F", "EC:2.7.4.8", "GO:0004385", "GO:0005524", "GO:0005829", "GO:0006163", "SO:0001217", "UniRef:UniRef50_P47353", "UniRef:UniRef90_P47353" ], "nt": "GTGAACAATCAGGGGAGAATTTTTGTCATTACTGGTCCTAGCGGTGTTGGCAAAAGCACCCTTGTTAAAGCCTTATTAGATCATTTCAAAGAACAACTGTTCTACAGTATCTCTGCAACTACAAGAAAAAAGCGCATTAGTGAAAAAGAGGGAATTGATTATTTTTTTAAAGATAAAGATGAGTTTGAAAACTTAATAAAACAAGATGCTTTCATTGAATGGGCTTGCTATAATAACCATTATTATGGAACGCTCAAGTCTCAAGCTGAACAAGCAATTAAAAGCGGAATTAATTTAATGCTTGAAATTGAGTATCAAGGTGCTTTACAGGTTAAAAGTAAATATCCTCATAACGTTGTTTTAATTTTCATTAAACCACCTTCAATGCAAGAGTTGTTAAAACGTTTAAAAAAGCGTAATGATGAAGATGAAACCACAATTAAAAAACGTTTAGAACAAGCTAAGATAGAGTTTCAACAGATTGATAATTTTAAGTATGTTGTCACTAACAAAGAGTTTGATAAAACCCTTAATGAGTTGAAATCAATCTTACTATCTGAGTTTATTTAA", "aa": "MNNQGRIFVITGPSGVGKSTLVKALLDHFKEQLFYSISATTRKKRISEKEGIDYFFKDKDEFENLIKQDAFIEWACYNNHYYGTLKSQAEQAIKSGINLMLEIEYQGALQVKSKYPHNVVLIFIKPPSMQELLKRLKKRNDEDETTIKKRLEQAKIEFQQIDNFKYVVTNKEFDKTLNELKSILLSEFI", "aa_hexdigest": "95bd95101c47f3b98b91effa28e4a3b9", "start_type": "GTG", "rbs_motif": null, "genes": [ "gmk" ], "truncated": "5-prime", "pseudogene": { "start": 134609, "stop": 135331, "observations": { "mutation": [ 134711 ], "stop": [ 134711 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47353", "query_cov": 1.0, "subject_cov": 0.7875, "identity": 0.995, "score": 367.0, "evalue": 1.71e-127, "gene_start": 1, "gene_end": 189, "reference_start": 52, "reference_end": 240, "reference_sequence": "MIQVSICSSIGKLTASVGLLIKALSSKTSQVLVGWKVIDKNSKVKIILTIEVNNQGRIFVITGPSGVGKSTLVKALLDHFKEQLFYSISATTRKKRISEKEGIDYFFKDKDEFENLIKQDAFIEWACYNNHYYGTLKSQAEQAIKSGINLMLEIEYQGALQVKSKYPHNVVLIFIKPPSMQELLKRLKKRNDEDETTIKKRLEQAKIEFQQIDNFKYVVTNKEFDKTLNELKSILLSEFI" }, "paralog": false, "identity": 0.9916666666666667, "score": 461.0, "evalue": 2.55e-167, "description": "Internal stop codon at 134711. Nonsense mutation around 134711.", "psc": { "uniref90_id": "UniRef90_P47353", "gene": "gmk", "product": "guanylate kinase", "ec_ids": [ "2.7.4.8" ], "uniref50_id": "UniRef50_P47353", "cog_id": "COG0194", "cog_category": "F", "go_ids": [ "GO:0004385", "GO:0005524", "GO:0005829", "GO:0006163" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47353", "EC:2.7.4.8", "UniRef:UniRef50_P47353", "COG:COG0194", "COG:F", "GO:0004385", "GO:0005524", "GO:0005829", "GO:0006163" ] }, "pscc": { "uniref50_id": "UniRef50_P47353", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47353" ], "product": "Guanylate kinase" } }, "id": "JPCNHPFPBM_277", "locus": "GCA000292505_00238" }, { "type": "cds", "sequence": "contig_1", "start": 135331, "stop": 135546, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGCTGCTAATGATCATTTCTTTACCGGTTTATCAAAAAAAGGTCCTGTAAGAAAGGAAAATCAGGACTTTTATGGATTTAGTTTTAATCAAAATAACCTACTAATAGTTGTTTGTGATGGTCTTGGTGGTTATAAGGGTGGCAAGATTGCTAGTAACTTAGTAGGTAAACTCTTTCTTAGTTTATTTGAAGGTTTTGAATTTAACCAGTGA", "aa": "MQAANDHFFTGLSKKGPVRKENQDFYGFSFNQNNLLIVVCDGLGGYKGGKIASNLVGKLFLSLFEGFEFNQ", "aa_hexdigest": "9d811b60877e9a5d207cc794be7488c2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7867.837799999999, "isoelectric_point": 7.879471015930176 }, "id": "JPCNHPFPBM_278", "locus": "GCA000292505_00239" }, { "type": "cds", "sequence": "contig_1", "start": 135799, "stop": 136113, "strand": "+", "frame": 1, "gene": "pTC1", "product": "Putative protein phosphatase", "db_xrefs": [ "COG:COG0631", "COG:T", "EC:3.1.3.16", "GO:0007165", "GO:0017018", "GO:0033192", "GO:0140791", "GO:0180004", "GO:0180005", "GO:0180006", "GO:0180007", "GO:0180008", "GO:1990439", "KEGG:K20074", "SO:0001217", "UniRef:UniRef50_P47354", "UniRef:UniRef90_P47354" ], "nt": "ATGAATGCTGATGAGAAATTACTTTTAAGTTATTCTAATCAACTTTTAGCATTAACAAATACTATTTCCAAAGAAACAAAAAGACCATTAGTTTATGGTTTTTATAACACAAAAATTGAACAACAAGAATTTTTATTATTGTGTTCTGATGGACTTTATAACTTTGTTGAAAAAGAGCTTTTTTTTGAAATTATCACTAATTCTAAAAACCTAAAACAAGCAGTGTTTAATCTTTATAGAAAAAGTATTGAAAATGCAAGTAATGATAATATTACTGCTGCCCTTGTTAATCTGCAAAAATGGAAGCAATCTTAA", "aa": "MNADEKLLLSYSNQLLALTNTISKETKRPLVYGFYNTKIEQQEFLLLCSDGLYNFVEKELFFEIITNSKNLKQAVFNLYRKSIENASNDNITAALVNLQKWKQS", "aa_hexdigest": "bb93c639f64d7b55e5df02060884917c", "start_type": "ATG", "rbs_motif": null, "genes": [ "pTC1" ], "truncated": "5-prime", "pseudogene": { "start": 135499, "stop": 136113, "observations": { "mutation": [ 135544, 135709 ], "stop": [ 135544, 135709 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47354", "query_cov": 1.0, "subject_cov": 0.4, "identity": 1.0, "score": 204.0, "evalue": 1.15e-64, "gene_start": 1, "gene_end": 104, "reference_start": 157, "reference_end": 260, "reference_sequence": "MQAANDHFFTGLSKKGPVRKENQDFYGFSFNQNNLLIVVCDGLGGYKGGKIASNLVGKLFLSLFEGFEFNQWDETTVKKWFENTLIQARFQLENCFQTVYEAQIQFARMASTLVLGILTKSDIYIFWIGDSRAYLLFENQAKLVTKDHNLYNQLVAMNADEKLLLSYSNQLLALTNTISKETKRPLVYGFYNTKIEQQEFLLLCSDGLYNFVEKELFFEIITNSKNLKQAVFNLYRKSIENASNDNITAALVNLQKWKQS" }, "paralog": false, "identity": 0.9901960784313726, "score": 396.0, "evalue": 1.53e-142, "description": "Internal stop codon at 135544, 135709. Nonsense mutation around 135544, 135709.", "psc": { "uniref90_id": "UniRef90_P47354", "gene": "PTC1", "product": "Putative protein phosphatase", "ec_ids": [ "3.1.3.16" ], "uniref50_id": "UniRef50_P47354", "cog_id": "COG0631", "cog_category": "T", "kegg_orthology_id": "K20074", "go_ids": [ "GO:0033192", "GO:0140791", "GO:1990439", "GO:0017018", "GO:0180006", "GO:0180007", "GO:0180008", "GO:0180005", "GO:0180004", "GO:0007165" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47354", "EC:3.1.3.16", "UniRef:UniRef50_P47354", "COG:COG0631", "COG:T", "KEGG:K20074", "GO:0033192", "GO:0140791", "GO:1990439", "GO:0017018", "GO:0180006", "GO:0180007", "GO:0180008", "GO:0180005", "GO:0180004", "GO:0007165" ] }, "pscc": { "uniref50_id": "UniRef50_P47354", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47354" ], "product": "Putative protein phosphatase" } }, "id": "JPCNHPFPBM_279", "locus": "GCA000292505_00240" }, { "type": "cds", "sequence": "contig_1", "start": 136098, "stop": 137021, "strand": "+", "frame": 3, "gene": "sPS1", "product": "Putative serine/threonine-protein kinase", "db_xrefs": [ "COG:COG0515", "COG:T", "EC:2.7.11.1", "GO:0004674", "GO:0004676", "GO:0004677", "GO:0004679", "GO:0004711", "GO:0005524", "GO:0005886", "GO:0007165", "GO:0035175", "GO:0035402", "GO:0035403", "GO:0035979", "GO:0044022", "GO:0044023", "GO:0044024", "GO:0044025", "GO:0072354", "GO:0072371", "GO:0072518", "GO:0106310", "GO:0140823", "GO:0140855", "GO:0140857", "GO:1990244", "SO:0001217", "UniRef:UniRef50_P47355", "UniRef:UniRef90_P47355" ], "nt": "ATGGAAGCAATCTTAAAGATAGGTGATATTGTTGAAAATAAGTATCAAATTGAAAAGCTTTTAAATAGGGGTGGGATGGATTCCTATCTTTTTTTAGCAAAAAATTTGAACTTAAAAAACTATGGACCAGTTCAAAAAAAACAGTATGGACACTTAGTTTTAAAGGTGGTTCAAAAAAATCCTAAGATTAATGAAAATAATTGGAAAAAGTTCCTTGATGAAATGGTAACCACAACTAGAGTGCACCATTCTAACCTAGTGAAAAGTTTTGATGTGGTGAACCCTTTTTTGAAAATAGTTCGTGGTAATAAAACAATTGCTCTCAATCAGATAGTGATGATTGCAATGGAGTATGTTGATGGCCCTTCTTTAAGACAATTGCTTAACAGAAAAGGCTACTTTAGTGTTAGCGAAGTAGTTTATTACTTTACAAAGATAGTAAAAGCCATTGATTATCTCCACAGTTTCAAGCACCAAATTATCCACCGTGATCTTAAACCTGAAAACATCTTATTTACTAGTGATTTAACTGATATTAAACTATTAGATTTTGGCATTGCTTCAACAGTAGTTAAAGTTGCTGAAAAAACCGAAGTTTTAACTGATGAAAATTCACTGTTTGGCACAGTTAGTTATATGATTCCTGATGTTTTGGAAAGCACAGTTAATAAAGCTGGTAAAAAGGTTAGAAAACCACCCAATGCCCAGTATGATATCTACTCGTTAGGAATTATTCTATTTGAGATGTTAGTTGGTAGAGTTCCATTTAACAAGTCAATTAACCCCAACAAGGAAAGAGAAACAATCCAAAAAGCACGCAATTTTGACTTACCTTTAATGCAAGCAACTAGAAGTGATATCCCAAATAGCTTAGAAAATATTGCTTTTCGTTGTACTGCTGTGAAAAGAGAAAACAATAAGTGA", "aa": "MEAILKIGDIVENKYQIEKLLNRGGMDSYLFLAKNLNLKNYGPVQKKQYGHLVLKVVQKNPKINENNWKKFLDEMVTTTRVHHSNLVKSFDVVNPFLKIVRGNKTIALNQIVMIAMEYVDGPSLRQLLNRKGYFSVSEVVYYFTKIVKAIDYLHSFKHQIIHRDLKPENILFTSDLTDIKLLDFGIASTVVKVAEKTEVLTDENSLFGTVSYMIPDVLESTVNKAGKKVRKPPNAQYDIYSLGIILFEMLVGRVPFNKSINPNKERETIQKARNFDLPLMQATRSDIPNSLENIAFRCTAVKRENNK", "aa_hexdigest": "3ae8cd8ba63e45f2ed275564bb089a3d", "start_type": "ATG", "rbs_motif": null, "genes": [ "sPS1" ], "truncated": "3-prime", "pseudogene": { "start": 136098, "stop": 137261, "observations": { "mutation": [ 137019, 137064, 137175 ], "stop": [ 137019, 137064, 137175 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47355", "query_cov": 1.0, "subject_cov": 0.7932816537467701, "identity": 1.0, "score": 602.0, "evalue": 4.8e-216, "gene_start": 1, "gene_end": 307, "reference_start": 1, "reference_end": 307, "reference_sequence": "MEAILKIGDIVENKYQIEKLLNRGGMDSYLFLAKNLNLKNYGPVQKKQYGHLVLKVVQKNPKINENNWKKFLDEMVTTTRVHHSNLVKSFDVVNPFLKIVRGNKTIALNQIVMIAMEYVDGPSLRQLLNRKGYFSVSEVVYYFTKIVKAIDYLHSFKHQIIHRDLKPENILFTSDLTDIKLLDFGIASTVVKVAEKTEVLTDENSLFGTVSYMIPDVLESTVNKAGKKVRKPPNAQYDIYSLGIILFEMLVGRVPFNKSINPNKERETIQKARNFDLPLMQATRSDIPNSLENIAFRCTAVKRENNKWLYSSTKELLEDLANWENEQAMIKPANERVLEGQVEIREMMLEKPLAWYFKTWALSIFTIVFIGLIIAAIVLLLIFNARF" }, "paralog": false, "identity": 0.9922480620155039, "score": 748.0, "evalue": 4.34e-276, "description": "Internal stop codon at 137019, 137064, 137175. Nonsense mutation around 137019, 137064, 137175.", "psc": { "uniref90_id": "UniRef90_P47355", "gene": "SPS1", "product": "Putative serine/threonine-protein kinase", "ec_ids": [ "2.7.11.1" ], "uniref50_id": "UniRef50_P47355", "cog_id": "COG0515", "cog_category": "T", "go_ids": [ "GO:0005886", "GO:0004676", "GO:0004679", "GO:0005524", "GO:0004677", "GO:0044024", "GO:0072371", "GO:1990244", "GO:0035979", "GO:0044025", "GO:0140823", "GO:0035175", "GO:0044022", "GO:0140855", "GO:0035402", "GO:0072354", "GO:0140857", "GO:0035403", "GO:0044023", "GO:0106310", "GO:0004674", "GO:0072518", "GO:0004711", "GO:0007165" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47355", "EC:2.7.11.1", "UniRef:UniRef50_P47355", "COG:COG0515", "COG:T", "GO:0005886", "GO:0004676", "GO:0004679", "GO:0005524", "GO:0004677", "GO:0044024", "GO:0072371", "GO:1990244", "GO:0035979", "GO:0044025", "GO:0140823", "GO:0035175", "GO:0044022", "GO:0140855", "GO:0035402", "GO:0072354", "GO:0140857", "GO:0035403", "GO:0044023", "GO:0106310", "GO:0004674", "GO:0072518", "GO:0004711", "GO:0007165" ] }, "pscc": { "uniref50_id": "UniRef50_P47355", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47355" ], "product": "Putative serine/threonine-protein kinase" } }, "id": "JPCNHPFPBM_280", "locus": "GCA000292505_00241" }, { "type": "cds", "sequence": "contig_1", "start": 137082, "stop": 137177, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAAACCTGCTAATGAAAGGGTTTTAGAAGGGCAGGTGGAAATTAGAGAAATGATGCTTGAAAAGCCCTTAGCTTGGTATTTCAAAACTTGA", "aa": "MIKPANERVLEGQVEIREMMLEKPLAWYFKT", "aa_hexdigest": "c33c19896e15df6b6658bc9374a8b804", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3751.4417000000003, "isoelectric_point": 6.0999265670776355 }, "id": "JPCNHPFPBM_281", "locus": "GCA000292505_00242" }, { "type": "cds", "sequence": "contig_1", "start": 137374, "stop": 137514, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGGTGATAGGGTTCAACTTGAAGATGGTGCGATCACTAAAGTTTTAGCTAGAAAAAATGAACTTACAAGACCAAGGGTTGCTAATGTTGATCAGATTGTTTTAATCCAATCACTAGTTCAACCTAAGATTAACTGA", "aa": "MVGDRVQLEDGAITKVLARKNELTRPRVANVDQIVLIQSLVQPKIN", "aa_hexdigest": "189b228caee193f8e7f40df2b8cfff7b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5139.973, "isoelectric_point": 9.687615776062014 }, "id": "JPCNHPFPBM_282", "locus": "GCA000292505_00243" }, { "type": "cds", "sequence": "contig_1", "start": 137524, "stop": 138051, "strand": "+", "frame": 1, "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "db_xrefs": [ "COG:COG1162", "COG:J", "EC:3.6.1.-", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0019843", "GO:0042274", "GO:0043022", "GO:0046872", "KEGG:K06949", "SO:0001217", "UniRef:UniRef50_P75523", "UniRef:UniRef90_P47356" ], "nt": "TTGAAATTATTGGTATATTTCAATGCTAAATTAATTGATGAAATACCTATTTTAATAACAAAAACAGACCTTGATTTTGATCCAATGGAAAAACAAAAGTTAATCGATTTAAAACAGTTTAACCATCAGTTATTTTTTGTTTCTAAAAATGAACCACTACCTTCAGAATTAATCGATATTTTTAGTAAAAAACTAAGTGTTTTTACAGGTCAATCTGGTGTTGGTAAATCTAGCTTAATTAATCGTTTAGATCCTTCTTTAAAACAAAAGATTCAAGCCTTATCAGTTAATAAATTTGGTAAGAATACCACTACTAAAACAACACTTTTTTCATTTAGAGGCGGTTTTATTTGTGACACCCCTGGTTTTAATGTAATTTCTATTAAAAACCTCAAAATTTTAGCAGCCCAACACTTTGTTGGTTTTCAGAAAATGATTAGTAAGTGTCATTTTTCTAACTGTTATCATCAGTATGAAAAAGATTGCTTTGTAACCACAAGTGTTATGAAAAACAGATATCCTTCGTGA", "aa": "MKLLVYFNAKLIDEIPILITKTDLDFDPMEKQKLIDLKQFNHQLFFVSKNEPLPSELIDIFSKKLSVFTGQSGVGKSSLINRLDPSLKQKIQALSVNKFGKNTTTKTTLFSFRGGFICDTPGFNVISIKNLKILAAQHFVGFQKMISKCHFSNCYHQYEKDCFVTTSVMKNRYPS", "aa_hexdigest": "df1c164bb85072e62fe11bf81362b4d1", "start_type": "TTG", "rbs_motif": null, "genes": [ "rsgA" ], "truncated": "both", "pseudogene": { "start": 137248, "stop": 138084, "observations": { "mutation": [ 137353, 137512, 138049 ], "stop": [ 137353, 137512, 138049 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47356", "query_cov": 1.0, "subject_cov": 0.6294964028776978, "identity": 0.9890000000000001, "score": 346.0, "evalue": 4.3e-119, "gene_start": 1, "gene_end": 175, "reference_start": 93, "reference_end": 267, "reference_sequence": "MPDSNFGIVLQSLAKQCKVFWNNQIITAFPQKKLQWKNDFKLMVGDRVQLEDGAITKVLARKNELTRPRVANVDQIVLIQSLVQPKINWIQLLKLLVYFNAKLIDEIPILITKTDLDFDPMEKQKLIDLKQFNYQLFFVSKNEPLPSELIDIFSKKLSVFTGQSGVGKSSLINRLDPSLKQKIQALSVNKFGKNTTTKTTLFSFRGGFICDTPGFNVISIKNLKILAAQHFVGFQKMISKCHFSNCYHQYEKDCFVTTSVMKNRYPSWLYEKYRKMIN" }, "paralog": false, "identity": 0.9856115107913669, "score": 541.0, "evalue": 1.86e-198, "description": "Internal stop codon at 137353, 137512, 138049. Nonsense mutation around 137353, 137512, 138049.", "psc": { "uniref90_id": "UniRef90_P47356", "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "ec_ids": [ "3.6.1.-" ], "uniref50_id": "UniRef50_P75523", "cog_id": "COG1162", "cog_category": "J", "kegg_orthology_id": "K06949", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0019843", "GO:0042274", "GO:0043022", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47356", "EC:3.6.1.-", "UniRef:UniRef50_P75523", "COG:COG1162", "COG:J", "KEGG:K06949", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0019843", "GO:0042274", "GO:0043022", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P75523", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75523" ], "product": "Small ribosomal subunit biogenesis GTPase RsgA" } }, "id": "JPCNHPFPBM_283", "locus": "GCA000292505_00244" }, { "type": "cds", "sequence": "contig_1", "start": 138105, "stop": 138860, "strand": "+", "frame": 3, "gene": "pgi", "product": "glucose-6-phosphate isomerase", "db_xrefs": [ "COG:COG0166", "COG:G", "EC:5.3.1.9", "GO:0004347", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0048029", "GO:0051156", "GO:0097367", "SO:0001217", "UniRef:UniRef50_P47357", "UniRef:UniRef90_P47357" ], "nt": "ATGAGTGATAAATTATTAACAATTGACTTAAGTCATGTTTATGGATTTGATAAAGAAATTATTTTTAAGAAATACCAAAAAAAAGTAGATCAAATTCACCAAGATTTTCTAGCTCATAAACTTGCTGATGGTCACATGACTGGGTGGTATGACCAACCTGATCAAAACCACCAATTCCTTTTAAAAACCATTAATCAAATTGACAAAAAGTTTAAAAGTTTAAAAGTAACTGACATTGTTTATGTTGGTATTGGTGGTTCTTTTACTGGTATTAAAACAGTTTTAGATTTCTTAAAACCAAAACAAAGAACAGGATTAAAAATCCACTTTGTCCCTGACCTTTCTGCTTTTCAAGCTGCAAGTGTTATTAAGGAAATTAAAAATAAATCATGGGCTCTAATTACCACTTCTAAGTCTGGTAGAACCCTAGAACCAGCACTGAATTTCCGCATTTTTAGAAACTTATTAAACAAGCGTTATGGCAACAAACACTACCAAAGAGTAGTTGTTATTACTGATGAAAAAAAGGGATTACTAACCAAAATGGCATCAAATCATGGTTACCAAAAGTTAGTTATTGATTCAAATATCGGTGGGCGTTTTTCAACTCTATCTCCTGCTGGTTTGTTACTAGCCAAACTTTTTGGTCATGATCCTAAGGCCATCTTAAAAGGAACATTACAAGCCAAAAAGGATTTGCAAACAACTTCACTTGAAAACAATTCTGCATACCTTTATGCAGTAGTTAGACATTGA", "aa": "MSDKLLTIDLSHVYGFDKEIIFKKYQKKVDQIHQDFLAHKLADGHMTGWYDQPDQNHQFLLKTINQIDKKFKSLKVTDIVYVGIGGSFTGIKTVLDFLKPKQRTGLKIHFVPDLSAFQAASVIKEIKNKSWALITTSKSGRTLEPALNFRIFRNLLNKRYGNKHYQRVVVITDEKKGLLTKMASNHGYQKLVIDSNIGGRFSTLSPAGLLLAKLFGHDPKAILKGTLQAKKDLQTTSLENNSAYLYAVVRH", "aa_hexdigest": "07eef0244e1c0be1a3f0164ea79da9dd", "start_type": "ATG", "rbs_motif": null, "genes": [ "pgi" ], "truncated": "3-prime", "pseudogene": { "start": 138105, "stop": 139163, "observations": { "mutation": [ 138858 ], "stop": [ 138858 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47357", "query_cov": 1.0, "subject_cov": 0.5823665893271461, "identity": 1.0, "score": 501.0, "evalue": 9.23e-177, "gene_start": 1, "gene_end": 251, "reference_start": 1, "reference_end": 251, "reference_sequence": "MSDKLLTIDLSHVYGFDKEIIFKKYQKKVDQIHQDFLAHKLADGHMTGWYDQPDQNHQFLLKTINQIDKKFKSLKVTDIVYVGIGGSFTGIKTVLDFLKPKQRTGLKIHFVPDLSAFQAASVIKEIKNKSWALITTSKSGRTLEPALNFRIFRNLLNKRYGNKHYQRVVVITDEKKGLLTKMASNHGYQKLVIDSNIGGRFSTLSPAGLLLAKLFGHDPKAILKGTLQAKKDLQTTSLENNSAYLYAVVRHWLYTTKKFKIEVCIAYHSLYEYLLLQHRQLFGESEGKNDKSLFPTFSIFTVDLHSMGQLYQEGEKVFFETVIDVKNPLVNINLPPSDFDNDDELDFLLDKSLNEISDVAIDSVIKAHYQANVSIIKLTLKEQSAFMFGYFYFWLSVATVMSGSLLGHNVFNQPGVEVYKKLMFEKLRSGH" }, "paralog": false, "identity": 0.9971590909090909, "score": 703.0, "evalue": 1.17e-258, "description": "Internal stop codon at 138858. Nonsense mutation around 138858.", "psc": { "uniref90_id": "UniRef90_P47357", "gene": "pgi", "product": "glucose-6-phosphate isomerase", "ec_ids": [ "5.3.1.9" ], "uniref50_id": "UniRef50_P47357", "cog_id": "COG0166", "cog_category": "G", "go_ids": [ "GO:0004347", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0048029", "GO:0051156", "GO:0097367" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47357", "EC:5.3.1.9", "UniRef:UniRef50_P47357", "COG:COG0166", "COG:G", "GO:0004347", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0048029", "GO:0051156", "GO:0097367" ] }, "pscc": { "uniref50_id": "UniRef50_P47357", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47357" ], "product": "Glucose-6-phosphate isomerase" } }, "id": "JPCNHPFPBM_284", "locus": "GCA000292505_00245" }, { "type": "cds", "sequence": "contig_1", "start": 138912, "stop": 139286, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATGAATATTTGTTATTACAGCATCGACAACTTTTTGGTGAATCAGAAGGTAAGAACGATAAATCTTTATTTCCTACTTTTTCGATTTTTACTGTTGACTTACACTCAATGGGACAACTCTATCAAGAAGGGGAAAAAGTGTTTTTTGAAACAGTAATTGATGTTAAAAATCCACTTGTTAATATTAATTTACCTCCATCTGATTTTGACAATGATGATGAACTTGATTTCTTGTTAGATAAAAGCTTAAATGAGATTTCAGATGTTGCAATTGATTCAGTTATTAAAGCGCACTACCAAGCAAATGTAAGCATTATTAAATTAACTTTAAAAGAACAATCTGCATTTATGTTTGGTTATTTTTACTTTTGA", "aa": "MYEYLLLQHRQLFGESEGKNDKSLFPTFSIFTVDLHSMGQLYQEGEKVFFETVIDVKNPLVNINLPPSDFDNDDELDFLLDKSLNEISDVAIDSVIKAHYQANVSIIKLTLKEQSAFMFGYFYF", "aa_hexdigest": "9c21bc0eb807d4b4ad1c0a13b1c0f4ec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14374.081199999979, "isoelectric_point": 4.418231391906739 }, "id": "JPCNHPFPBM_285", "locus": "GCA000292505_00246" }, { "type": "cds", "sequence": "contig_1", "start": 139390, "stop": 140019, "strand": "+", "frame": 1, "gene": "rpe", "product": "Ribulose-phosphate 3-epimerase", "db_xrefs": [ "COG:COG0036", "COG:G", "EC:5.1.3.1", "GO:0004750", "GO:0005829", "GO:0005975", "GO:0009052", "GO:0019323", "GO:0046872", "RefSeq:WP_009885669.1", "SO:0001217", "UniParc:UPI0000134621", "UniRef:UniRef100_P47358", "UniRef:UniRef50_P47358", "UniRef:UniRef90_P47358" ], "nt": "GTGGCCACTAAGGTTGTTTTTTCACTCTTACCACTTTTAAATAGGTTTGACAAGTCACTTTTAGAAAGTTACTTTCAAGATGGATTGAGGTTAATCCATTATGATGTGATGGACCAATTTGTTCATAATACTGCTTTTAAAGGTGAATATTTGGATGAATTGAAAACAATAGGTTTTGATGTTAATGTCCATTTAATGGTGGAACAGATCATCCCTCAAATAAATTTTTATCTTTCACAACCTAATGTGAAAAGGATTTCGTTTCATGTTGAACCATTTAGTTTTGCAAAGATTAAAGAACTAATCCAACTAGTTAAAGAAAATGGTAAAGAAGTTGGTCTTGCTTTTAAATTTACAACCAATTTACAACTATACCAACCATTTTTTACAACCATCGACTTTATCACTTTAATGAGTGTTCCTCCTGGTAAAGGTGGTCAAGCTTTTAACGAAGCTGTTTTTACAAATTTAAAGATTGCTAACCATTACAACTTGAAAATTGAGATTGATGGTGGGATTAAAGTTAATAACATTGATCAAATTAAAGCCTTTGTTGATTTCATTGTAATGGGAAGTGGCTTTATAAAATTAGAGCAGTGGCAACGTCAAAAATTGTTGCAAACAATCTAA", "aa": "MATKVVFSLLPLLNRFDKSLLESYFQDGLRLIHYDVMDQFVHNTAFKGEYLDELKTIGFDVNVHLMVEQIIPQINFYLSQPNVKRISFHVEPFSFAKIKELIQLVKENGKEVGLAFKFTTNLQLYQPFFTTIDFITLMSVPPGKGGQAFNEAVFTNLKIANHYNLKIEIDGGIKVNNIDQIKAFVDFIVMGSGFIKLEQWQRQKLLQTI", "aa_hexdigest": "3e9c16fedfdc638449e854ff97b054d3", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134621", "ncbi_nrp_id": "WP_009885669.1", "uniref100_id": "UniRef100_P47358", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134621", "RefSeq:WP_009885669.1", "UniRef:UniRef100_P47358" ] }, "ips": { "uniref100_id": "UniRef100_P47358", "uniref90_id": "UniRef90_P47358" }, "psc": { "uniref90_id": "UniRef90_P47358", "gene": "rpe", "product": "Ribulose-phosphate 3-epimerase", "ec_ids": [ "5.1.3.1" ], "uniref50_id": "UniRef50_P47358", "cog_id": "COG0036", "cog_category": "G", "go_ids": [ "GO:0005829", "GO:0004750", "GO:0046872", "GO:0005975", "GO:0019323", "GO:0009052" ] }, "pscc": { "uniref50_id": "UniRef50_P47358", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47358" ], "product": "Ribulose-phosphate 3-epimerase" }, "genes": [ "rpe" ], "id": "JPCNHPFPBM_286", "locus": "GCA000292505_00247" }, { "type": "cds", "sequence": "contig_1", "start": 140033, "stop": 140266, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01336.31" ], "nt": "ATGAAATCAGTTACAGTCAAGCAGTTACTACAAACCCCACGAAAATTTAATAACAAGCAGATTAAACTATCAGGTTGGGTTAAAAATAAACGTGCTAGTGCTAACATCATCTTTCTAGCAATTAGTGATGGCTCTAGTATTAATACCCTACAAGCAGTAGTAAAACAAGAAGATAACCCCCAGGTTTTCTCACTGTTACAAACTGTTAATTTAGCAAGTGCTGTTATGGTTTGA", "aa": "MKSVTVKQLLQTPRKFNNKQIKLSGWVKNKRASANIIFLAISDGSSINTLQAVVKQEDNPQVFSLLQTVNLASAVMV", "aa_hexdigest": "e9b27030ada31fc5733be86c96b6a892", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01336.31", "name": "tRNA_anti-codon", "length": 47, "aa_cov": 0.5974025974025974, "hmm_cov": 0.9574468085106383, "evalue": 4.453786712059396e-08, "score": 28.1107120513916, "start": 22, "stop": 67 } ], "seq_stats": { "molecular_weight": 8499.862000000001, "isoelectric_point": 10.461107444763183 }, "id": "JPCNHPFPBM_287", "locus": "GCA000292505_00248" }, { "type": "cds", "sequence": "contig_1", "start": 140324, "stop": 141259, "strand": "+", "frame": 2, "gene": "asnS", "product": "asparagine--tRNA ligase", "db_xrefs": [ "COG:COG0017", "COG:J", "EC:6.1.1.22", "GO:0000166", "GO:0003676", "GO:0004816", "GO:0005524", "GO:0005737", "GO:0006421", "KEGG:K01893", "SO:0001217", "UniRef:UniRef50_P47359", "UniRef:UniRef90_P47359" ], "nt": "GTGAGTTTATTAGCACAAGCAGAGTCTGATTATCCACTGCAAAAAAAAGAACATAGTCAAGAGTTTTTTAGAAGTAATGCGCATCTAAGAGTAAGAGCAAAAACTTACTTTGCAGTGATGAAAATAAGGAGTGTTTTGTCACACGCAATCTTTGAATACTTCTTTAAAAATGATTTTATCTTAGTGCAAAGCCCTATTTTAACTAGTAATGATTGTGAGGGAGCGGGGGAAACATTTGTAATTAAAGATAGTGAAACTTTTTTTAATAAAACGACTTTTTTAACAGTAAGTGGCCAGTTTGGAGCAGAAGCTTTTGCGCAAGCATTTAAAAAGGTTTTCACCTTTGGTCCTACTTTCAGAGCTGAAAAATCCCATACTAATCGTCATCTTAGTGAGTTTTGGATGATCGAACCTGAAATTGCATTTGCTAACTTAAAAGATTTAATGCAGTTAATACAAAACCTAATTAAATTCTTAATTAAAAAAGTGATGAAAAATGCTAGTGATGAACTAAATGTTTTAGCAAAGCAATTTAGCAATGACATTATTAGCAACTTAAAGACAATCATTAGTACTAAAAAATTTCCAATCATTGAATACAGCAAAGCATTAGCGATTCTAAAGGAATCTAGTGATACAAAAAAAACTAATTTTGAACTAAACGACTTTAGTTTTGGTATTGACTTAAAAACAGAACATGAACGCTTTTTGTGCGAACAATATTTTCAAAATCAACCGCTTTTTGTTATTAACTATCCAAAGGAGTTAAAGGCATTTTACATGAAAACAAATACTGACAATAAAACTGTTGCTGCAGTTGATCTTTTATTACCAAAGATTGGTGAGATTTGTGGGGGAAGTGAAAGGGAAAGTGATTTAAACCAACTTAAGAATAGGTGTCAATCTTTAAACATTGACACAAAAAGTTTGAACTGA", "aa": "MSLLAQAESDYPLQKKEHSQEFFRSNAHLRVRAKTYFAVMKIRSVLSHAIFEYFFKNDFILVQSPILTSNDCEGAGETFVIKDSETFFNKTTFLTVSGQFGAEAFAQAFKKVFTFGPTFRAEKSHTNRHLSEFWMIEPEIAFANLKDLMQLIQNLIKFLIKKVMKNASDELNVLAKQFSNDIISNLKTIISTKKFPIIEYSKALAILKESSDTKKTNFELNDFSFGIDLKTEHERFLCEQYFQNQPLFVINYPKELKAFYMKTNTDNKTVAAVDLLLPKIGEICGGSERESDLNQLKNRCQSLNIDTKSLN", "aa_hexdigest": "f7e71184d13ab4855d00a20b2ee4d87b", "start_type": "GTG", "rbs_motif": null, "genes": [ "asnS" ], "truncated": "both", "pseudogene": { "start": 140033, "stop": 141403, "observations": { "mutation": [ 140264, 141257 ], "stop": [ 140264, 141257 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47359", "query_cov": 1.0, "subject_cov": 0.6820175438596491, "identity": 0.9940000000000001, "score": 601.0, "evalue": 9.84e-215, "gene_start": 1, "gene_end": 311, "reference_start": 98, "reference_end": 408, "reference_sequence": "MKSVTVKQLLQTPRKFNNKQIKLSGWVKNKRASANIIFLAISDGSSINTLQAVVKQEDNPQVFSLLQTVNLASAVMVWGEIILTPKAKQPLELKLKQVSLLAQAESDYPLQKKEHSQEFFRSNAHLRVRAKTYFAVMKIRSVLSHAIFEYFFKNDFILVQSPILTSNDCEGAGETFVIKDSETFFNKTTFLTVSGQFGAEAFAQAFKKVFTFGPTFRAEKSHTNRHLSEFWMIEPEIAFANLKDLMQLIQNLIKFLIKKVMENASDELNVLAKQFSNDIISNLKTIISTKKFPIIEYSKALAILKESSDTKKTNFELNDFSFGIDLKTEHERFLCEQYFQNQPLFVINYPKELKAFYMKTNTDNKTVAAVDLLLPKIGEICGGSERESDLNQLKNRCQSLNIDTKSLNWYLDMRKWGYFASAGFGLGFDRLLAYICGLENIRDAIPFPRVHGTINF" }, "paralog": false, "identity": 0.993421052631579, "score": 890.0, "evalue": 0.0, "description": "Internal stop codon at 140264, 141257. Nonsense mutation around 140264, 141257.", "psc": { "uniref90_id": "UniRef90_P47359", "gene": "asnS", "product": "asparagine--tRNA ligase", "ec_ids": [ "6.1.1.22" ], "uniref50_id": "UniRef50_P47359", "cog_id": "COG0017", "cog_category": "J", "kegg_orthology_id": "K01893", "go_ids": [ "GO:0000166", "GO:0003676", "GO:0004816", "GO:0005524", "GO:0005737", "GO:0006421" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47359", "EC:6.1.1.22", "UniRef:UniRef50_P47359", "COG:COG0017", "COG:J", "KEGG:K01893", "GO:0000166", "GO:0003676", "GO:0004816", "GO:0005524", "GO:0005737", "GO:0006421" ] }, "pscc": { "uniref50_id": "UniRef50_P47359", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47359" ], "product": "Asparagine--tRNA ligase" } }, "id": "JPCNHPFPBM_288", "locus": "GCA000292505_00249" }, { "type": "cds", "sequence": "contig_1", "start": 141269, "stop": 141403, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGAGGAAATGGGGTTATTTTGCTAGTGCAGGTTTTGGTTTGGGCTTTGATAGATTATTAGCTTATATATGTGGATTGGAAAACATCAGAGATGCTATTCCCTTTCCCCGTGTACATGGCACCATTAACTTCTAA", "aa": "MRKWGYFASAGFGLGFDRLLAYICGLENIRDAIPFPRVHGTINF", "aa_hexdigest": "fe5196cc5a7e35d0d9f2fd7fa4dfda18", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A378BFT2", "query_cov": 1.0, "subject_cov": 0.9166666666666666, "identity": 0.5, "score": 55.5, "evalue": 2.13e-09, "valid": false, "product": "Asparaginyl-tRNA synthetase", "uniref50_id": "UniRef50_A0A378BFT2" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 37, "aa_cov": 0.8409090909090909, "hmm_cov": 1.0, "evalue": 1.8515499994351078e-11, "score": 38.68692398071289, "start": 2, "stop": 38 } ], "seq_stats": { "molecular_weight": 4991.7507, "isoelectric_point": 9.183408546447755 }, "id": "JPCNHPFPBM_289", "locus": "GCA000292505_00250" }, { "type": "cds", "sequence": "contig_1", "start": 141360, "stop": 141662, "strand": "+", "frame": 3, "gene": "pgsA", "product": "CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase", "db_xrefs": [ "COG:COG0558", "COG:I", "EC:2.7.8.5", "GO:0005886", "GO:0006655", "GO:0008444", "GO:0008654", "GO:0016020", "GO:0046474", "SO:0001217", "UniRef:UniRef50_P47360", "UniRef:UniRef90_P47360" ], "nt": "ATGCTATTCCCTTTCCCCGTGTACATGGCACCATTAACTTCTAAATTCGCTGCTTATAAAAAAAAGATTGCAAACTGGTTAACAGTTTACAGAATTTTTATTGCTTTACCTACTATTATTTTTATTGCTTTAGATAATCAACTAGGAGTTTTAGCTAACTTTTCTGTTGGTGCAATTAGCATTAGTTTACAGATCAGTTTATTGATTGGAGGATTTTTGTTTTTAACTGCAGTTATATCAGATTATTTAGATGGATATTTAGCAAGAAAATGGCTAGCAGTTTCTAACTTTGGTAAATTATGA", "aa": "MLFPFPVYMAPLTSKFAAYKKKIANWLTVYRIFIALPTIIFIALDNQLGVLANFSVGAISISLQISLLIGGFLFLTAVISDYLDGYLARKWLAVSNFGKL", "aa_hexdigest": "fb905faad3c5ae4d0b8c9279a7c59a79", "start_type": "ATG", "rbs_motif": null, "genes": [ "pgsA" ], "truncated": "3-prime", "pseudogene": { "start": 141339, "stop": 141965, "observations": { "mutation": [ 141660, 141825, 141837, 141936 ], "stop": [ 141660, 141825, 141837, 141936 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47360", "query_cov": 1.0, "subject_cov": 0.423728813559322, "identity": 1.0, "score": 191.0, "evalue": 1.35e-59, "gene_start": 1, "gene_end": 100, "reference_start": 8, "reference_end": 107, "reference_sequence": "MDWKTSEMLFPFPVYMAPLTSKFAAYKKKIANWLTVYRIFIALPTIIFIALDNQLGVLANFSVGAISISLQISLLIGGFLFLTAVISDYLDGYLARKWLAVSNFGKLWDPIADKVIINGVLIALAINGYFHFSLLIVFIVRDLVLDGMRIYAYEKKVVIAANWLGKWKTIMQMVGIVFSCFVWSFKQSEIASLNSGLFFWLLTQLPYYLAAVFSIWSFIVYNIQIYQQLKAYNSKL" }, "paralog": false, "identity": 0.9759615384615384, "score": 393.0, "evalue": 9.21e-142, "description": "Internal stop codon at 141660, 141825, 141837, 141936. Nonsense mutation around 141660, 141825, 141837, 141936.", "psc": { "uniref90_id": "UniRef90_P47360", "gene": "pgsA", "product": "CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase", "ec_ids": [ "2.7.8.5" ], "uniref50_id": "UniRef50_P47360", "cog_id": "COG0558", "cog_category": "I", "go_ids": [ "GO:0005886", "GO:0006655", "GO:0008444", "GO:0008654", "GO:0016020", "GO:0046474" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47360", "EC:2.7.8.5", "UniRef:UniRef50_P47360", "COG:COG0558", "COG:I", "GO:0005886", "GO:0006655", "GO:0008444", "GO:0008654", "GO:0016020", "GO:0046474" ] }, "pscc": { "uniref50_id": "UniRef50_P47360", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47360" ], "product": "CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase" } }, "id": "JPCNHPFPBM_290", "locus": "GCA000292505_00251" }, { "type": "cds", "sequence": "contig_1", "start": 141681, "stop": 141827, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTATCAATGGTGTTCTTATTGCACTAGCGATTAATGGATATTTTCACTTTAGCTTATTAATTGTTTTTATAGTCCGTGATCTTGTGTTGGATGGAATGCGGATTTATGCTTATGAGAAAAAGGTGGTTATTGCTGCTAACTGA", "aa": "MIINGVLIALAINGYFHFSLLIVFIVRDLVLDGMRIYAYEKKVVIAAN", "aa_hexdigest": "ea3f372f1427cd7cb5a01ce016ab5adc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5411.513300000001, "isoelectric_point": 8.16035976409912 }, "id": "JPCNHPFPBM_291", "locus": "GCA000292505_00252" }, { "type": "cds", "sequence": "contig_1", "start": 142074, "stop": 142547, "strand": "+", "frame": 3, "gene": "pncC", "product": "Protein MG115", "db_xrefs": [ "COG:COG1546", "COG:H", "EC:3.5.1.42", "KEGG:K03743", "RefSeq:WP_009885673.1", "SO:0001217", "UniParc:UPI00001392B9", "UniRef:UniRef100_P47361", "UniRef:UniRef50_P47361", "UniRef:UniRef90_P47361" ], "nt": "TTGTTTGCCAATTTAATAGCAGAAAAACTCCAAAAGTTACAACTTAGTGTTGCAACAGCTGAATCAGTTACTGGTGGCTTATTAGCTCATTGTTTAACTTCCATTGATGGTGCTTCTAACTATTTTAATGGCGGTGTTATTGCTTACAATAACCAAGTTAAGATTAACTTACTGAATGTTCAATCCTCCACAATTGCAAACCATGGGGCAGTTTCCAGTTTCTGTGCTAGGGAGATGGCAGTTGGGGTTAAACAAAAGTTTCAAGCTGATGTGGGTATTGCTTGCAGTGGGATAGCAGGTAGTAAAGCAGTTGAAAATAAAGCAATAGGATTACTTTTTTTCTGTATTATTATAGGAAATAAGGCTTATGATTTTGAGTTTGAAATGAACCAAAATAATCGTAAGGATAACATTGAATTATTTACCAATAAGATCTTGGAATCTTTCCACTATTTGTTAACAAAGCTAGCTTAA", "aa": "MFANLIAEKLQKLQLSVATAESVTGGLLAHCLTSIDGASNYFNGGVIAYNNQVKINLLNVQSSTIANHGAVSSFCAREMAVGVKQKFQADVGIACSGIAGSKAVENKAIGLLFFCIIIGNKAYDFEFEMNQNNRKDNIELFTNKILESFHYLLTKLA", "aa_hexdigest": "ee88f60c5e80f1baaaefcce45f207bc5", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001392B9", "ncbi_nrp_id": "WP_009885673.1", "uniref100_id": "UniRef100_P47361", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001392B9", "RefSeq:WP_009885673.1", "UniRef:UniRef100_P47361" ] }, "ips": { "uniref100_id": "UniRef100_P47361", "uniref90_id": "UniRef90_P47361" }, "psc": { "uniref90_id": "UniRef90_P47361", "gene": "PncC", "product": "Protein MG115", "ec_ids": [ "3.5.1.42" ], "uniref50_id": "UniRef50_P47361", "cog_id": "COG1546", "cog_category": "H", "kegg_orthology_id": "K03743" }, "pscc": { "uniref50_id": "UniRef50_P47361", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47361" ], "product": "Protein MG115" }, "genes": [ "pncC" ], "id": "JPCNHPFPBM_292", "locus": "GCA000292505_00253" }, { "type": "cds", "sequence": "contig_1", "start": 142556, "stop": 143089, "strand": "+", "frame": 2, "gene": "ispD", "product": "putative protein MG116", "db_xrefs": [ "COG:COG1211", "COG:I", "SO:0001217", "UniRef:UniRef50_P75519", "UniRef:UniRef90_P47362" ], "nt": "ATGAAACGTACTTTAAATATAGGTATTGTTTTGTGTGAAAATTTTCTTTCAGACCAACAAAATGCTGTTGATAGTTACACCCAAGTTTATGAAGATGTTAGGATGTTTGAATTTGGGTTAAAACTCTTTCAATCATTACCTTTTAACATCCAAGAAACCCTTATATTTTGTAATGCTGAACAACATAAAATAGTTGATAAAGCTGCTAAAAAATACAAAAACACTACTGTGTTTTTTTCACGTGATACTGATGTAGCTAATGTTTATGAAGCTAAGGTATTTATCCAAGAAAAATACAAATTAACCCAAGATTACAAAAAAAGAGGTGTTAGCTCTTATTATGACAGCTGTTGCTTTATCTTAATTGAAGCGAATCGTCCATTAACTGCAATAAAGACAGTTAAAAGTGTTTATGAAAAAGCACTGATAGAAAAAGCAGCTATTGCAGTATTACCATATAATGGTACACTAATGAATGGTAACAATGATGTTGTCTTTAGCCACTTGCAAGATAAAAATAGGTTTAACTATTGA", "aa": "MKRTLNIGIVLCENFLSDQQNAVDSYTQVYEDVRMFEFGLKLFQSLPFNIQETLIFCNAEQHKIVDKAAKKYKNTTVFFSRDTDVANVYEAKVFIQEKYKLTQDYKKRGVSSYYDSCCFILIEANRPLTAIKTVKSVYEKALIEKAAIAVLPYNGTLMNGNNDVVFSHLQDKNRFNY", "aa_hexdigest": "f6ec4c09c526c983565036166bbab5d9", "start_type": "ATG", "rbs_motif": null, "genes": [ "ispD" ], "truncated": "3-prime", "pseudogene": { "start": 142556, "stop": 143311, "observations": { "mutation": [ 143087 ], "stop": [ 143087 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47362", "query_cov": 1.0, "subject_cov": 0.7051792828685259, "identity": 1.0, "score": 354.0, "evalue": 1.16e-122, "gene_start": 1, "gene_end": 177, "reference_start": 1, "reference_end": 177, "reference_sequence": "MKRTLNIGIVLCENFLSDQQNAVDSYTQVYEDVRMFEFGLKLFQSLPFNIQETLIFCNAEQHKIVDKAAKKYKNTTVFFSRDTDVANVYEAKVFIQEKYKLTQDYKKRGVSSYYDSCCFILIEANRPLTAIKTVKSVYEKALIEKAAIAVLPYNGTLMNGNNDVVFSHLQDKNRFNYWKQSKAYEVQYPQAYTLNKLNQFSKQQFLRARSMLDLMKISNKSPLSIVDGSAYAFRVVTNLDFEILLGILKNG" }, "paralog": false, "identity": 0.9920318725099602, "score": 493.0, "evalue": 8.02e-180, "description": "Internal stop codon at 143087. Nonsense mutation around 143087.", "psc": { "uniref90_id": "UniRef90_P47362", "gene": "IspD", "product": "Uncharacterized protein MG116", "uniref50_id": "UniRef50_P75519", "cog_id": "COG1211", "cog_category": "I", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47362", "UniRef:UniRef50_P75519", "COG:COG1211", "COG:I" ] }, "pscc": { "uniref50_id": "UniRef50_P75519", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75519" ], "product": "Uncharacterized protein MG116 homolog" } }, "id": "JPCNHPFPBM_293", "locus": "GCA000292505_00254" }, { "type": "cds", "sequence": "contig_1", "start": 143186, "stop": 143311, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGGACTTAATGAAGATTTCTAATAAATCACCCTTAAGTATTGTTGATGGTAGTGCTTATGCTTTTAGGGTTGTTACCAACCTTGATTTTGAAATTTTATTAGGTATTTTAAAAAATGGATAA", "aa": "MLDLMKISNKSPLSIVDGSAYAFRVVTNLDFEILLGILKNG", "aa_hexdigest": "8af0f9090e7692277155f57c0d623cce", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4497.279299999998, "isoelectric_point": 5.91951961517334 }, "id": "JPCNHPFPBM_294", "locus": "GCA000292505_00255" }, { "type": "cds", "sequence": "contig_1", "start": 143304, "stop": 143507, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAAAATATTTTTCAATTAGTACAAAGTTTTGCTAAAACGCAAAATGTGCGAGCTAATTACACCTCACAAGACAAAAATATTAGTTTAGATTTTGTTAGTTTTGAAACAGTTAGTCAATCACTAACAGGTTTTCTAATTTTTAATAATTTCAATAAATTGTTACAACTAATTGAACTGATAAAACAAAAGCAAACATGA", "aa": "MDKNIFQLVQSFAKTQNVRANYTSQDKNISLDFVSFETVSQSLTGFLIFNNFNKLLQLIELIKQKQT", "aa_hexdigest": "695f58ce4a6841392ec802bbf66dbe4e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7789.825799999995, "isoelectric_point": 9.22402362823486 }, "id": "JPCNHPFPBM_295", "locus": "GCA000292505_00256" }, { "type": "cds", "sequence": "contig_1", "start": 143595, "stop": 143948, "strand": "+", "frame": 3, "gene": null, "product": "MPN256 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47363", "UniRef:UniRef90_P47363" ], "nt": "TTGCCTGTTTCAGTTGCGGCTTTTAGCAACCAACAACTCAACCAAGTTTATCGCAATTCATCCACAAGTCCTTTGTATCTTAGTTATGTCAAACCAATCGAAGTTCAACAATTTTTTACACTTTCTCCATCCATTAACAATAATCCTTTTTTAAATCAAAATCCTTTAACTGAATCACCATTTGATAACAATAATCAGCTGTTTCAAGCAACAAAGAGCGTTGAACCTTCAATGGAAACAATGGAATTTTCCCGGTTTATGGATGAATTTGATCAGATTACAAAGAACTTTTCTGATATAGAGCTTGAACCTATGCAATTCACCCAAAGTTTTGATGATTGGAGCAAAGACTAG", "aa": "MPVSVAAFSNQQLNQVYRNSSTSPLYLSYVKPIEVQQFFTLSPSINNNPFLNQNPLTESPFDNNNQLFQATKSVEPSMETMEFSRFMDEFDQITKNFSDIELEPMQFTQSFDDWSKD", "aa_hexdigest": "20fe9c081e0b955f3813d61bd343d2d2", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 143304, "stop": 143948, "observations": { "mutation": [ 143505, 143526 ], "stop": [ 143505, 143526 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47363", "query_cov": 1.0, "subject_cov": 0.5467289719626168, "identity": 0.991, "score": 229.0, "evalue": 8.6e-75, "gene_start": 1, "gene_end": 117, "reference_start": 98, "reference_end": 214, "reference_sequence": "MDKNIFQLVQSFAKTQNVRANYTSQDKNISLDFVSFETVSQSLTGFLIFNNFNKLLQLIELIKQKQTWLYVDQLWIVDLSNNKALTDATVWIIKQEKLPVSVAAFSNQQLNQVYRNSSTSPLYLSYVKPIEVQQFFTLSPSINNNPFLNQNPLTESPFDNNNQLFQATKSVEPSMETMEFSRFMDEFDQITKNFSDIELEPMQFTQSFDDWSKD" }, "paralog": false, "identity": 0.9906542056074766, "score": 417.0, "evalue": 2.93e-151, "description": "Internal stop codon at 143505, 143526. Nonsense mutation around 143505, 143526.", "psc": { "uniref90_id": "UniRef90_P47363", "product": "MPN256 family protein", "uniref50_id": "UniRef50_P47363", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47363", "UniRef:UniRef50_P47363" ] }, "pscc": { "uniref50_id": "UniRef50_P47363", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47363" ], "product": "Uncharacterized protein MG117" } }, "id": "JPCNHPFPBM_296", "locus": "GCA000292505_00257" }, { "type": "cds", "sequence": "contig_1", "start": 143929, "stop": 144669, "strand": "+", "frame": 1, "gene": null, "product": "NAD-dependent epimerase/dehydratase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557C9D", "UniRef:UniRef90_UPI0000557C9D" ], "nt": "ATGATTGGAGCAAAGACTAGGGTTGCAATAGTTGGCGGGATTGGTTACATAGGTAGTTGTTTTGCTAGTTTTATCAAAGAACAAAATGATAAGCTAATTGTTACTGTTATTGATAACAACAAAAATAACCATGTAATTAAACTCTTAAAAAAGATTGGAATTGAATTCTATTTTGCTGATTTACTAGATAGACATAAGCTAACTGAAGTAATTGCAGCAATTCAACCTGATGTGGTATTTCACTTTGCTGCTAAAACAAGTGTAAGTGAATCAGTACATAATCCATTGAAGTACTTTGATTGCAATGTAATTGGTACTTTAAACCTAATTAGTGCAATTAGTAACTTACAGAAGCCAATTAAATTATTTTTCGCTTCTAGTGCTGCAGTGTATGGTCAAACAACTAATAGTTACATTAGTGAAGAGATTGTAATAACTGAAACACAAGCAACCAATCCTTATGGATTGAGTAAGTTTTTAGATGAATTAATCTTAAATGCAGTTGCCAAAAATAGTCAACTACAAGTTGTTTGCTTACGCTTTTTTAATGTGGCAGGTGCAATTCTGCCATTTGGTAATTTTAATGGTAATACCACGCTTTTAATTCCTAACTTAGTAAAAGCCTTTTTAAAACAAACTCCCTTTTTTTTATATGGCAATGATTATGCAACTAAGGATGGTAGTTGCATAAGAGATTACATCCATGTTTATGATATATGTAATGCTCATTTCTTATTATGA", "aa": "MIGAKTRVAIVGGIGYIGSCFASFIKEQNDKLIVTVIDNNKNNHVIKLLKKIGIEFYFADLLDRHKLTEVIAAIQPDVVFHFAAKTSVSESVHNPLKYFDCNVIGTLNLISAISNLQKPIKLFFASSAAVYGQTTNSYISEEIVITETQATNPYGLSKFLDELILNAVAKNSQLQVVCLRFFNVAGAILPFGNFNGNTTLLIPNLVKAFLKQTPFFLYGNDYATKDGSCIRDYIHVYDICNAHFLL", "aa_hexdigest": "75b92aaa9fb3f53da333954460ea821d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0000557C9D", "query_cov": 0.8780487804878049, "subject_cov": 0.9432314410480349, "identity": 1.0, "score": 424.0, "evalue": 1.59e-149, "valid": true, "product": "NAD-dependent epimerase/dehydratase family protein", "uniref50_id": "UniRef50_UPI0000557C9D" }, "pscc": { "uniref50_id": "UniRef50_UPI0000557C9D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557C9D" ], "product": "NAD-dependent epimerase/dehydratase family protein" }, "genes": [], "id": "JPCNHPFPBM_297", "locus": "GCA000292505_00258" }, { "type": "cds", "sequence": "contig_1", "start": 144715, "stop": 144951, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000319466E", "UniRef:UniRef90_UPI000319466E" ], "nt": "TTGGGGAGTGGGATAGGAACTTCTAATTTAGAAGTTATTGATATTGCTAAAAAAGTGTTTTATCCTAGTAGATTAAATTTAGAAATTAGACCAAAAAGAAGCAGAGATCCAGCAATTTTAGTAGCAAATGTTGCTAAAGCAAAACAAACCTTTCAATTCAAAATAACGCGTAATTTGAAAGATATGATAAGTGATGAGCGTAATTTTTATGAGAATTTTTATAATGACGCTTATTAA", "aa": "MGSGIGTSNLEVIDIAKKVFYPSRLNLEIRPKRSRDPAILVANVAKAKQTFQFKITRNLKDMISDERNFYENFYNDAY", "aa_hexdigest": "7a4712f8b9a58cb97831a4c768c1a83d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000319466E", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.987, "score": 152.0, "evalue": 6.68e-47, "valid": true, "uniref50_id": "UniRef50_UPI000319466E" }, "pscc": { "uniref50_id": "UniRef50_UPI000319466E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000319466E" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9032.239299999997, "isoelectric_point": 9.57015438079834 }, "id": "JPCNHPFPBM_298", "locus": "GCA000292505_00259" }, { "type": "cds", "sequence": "contig_1", "start": 144966, "stop": 146129, "strand": "+", "frame": 3, "gene": "nupO", "product": "Putative carbohydrate transport ATP-binding protein MG119", "db_xrefs": [ "COG:COG3845", "COG:F", "GO:0005524", "GO:0005886", "GO:0016887", "KEGG:K23537", "SO:0001217", "UniRef:UniRef50_P47365", "UniRef:UniRef90_P47365" ], "nt": "ATGGAAAAAGTTGCCTTCAAAATGGAGCATATCTCCAAAAGTTTTGACAATGGCAAAATTAAGGCTAATGTTGATGTTAGCTTAGTTGTTTATGAAAATACTGTCCACACCATTTTGGGGGAGAATGGTGCAGGAAAATCAACCCTGACTTCGATTTTATTTGGTTTATATAAACCTGATAGTGGCAAGATCTTTATTGGTGAAAAGCAAGTAAATTTTAAATCTTCTAAAGATGCAGTAAAACATAAAATCGGAATGGTGCACCAGCACTTTAAGTTAATAGAAAACTACACGGTTTTAGATAACATCATTCTAGGGAATGAAAGTAGGTTTGGGTTTTTACCTTTAATTAATCGTAAAGTAAGTGAAGCAAAGATTAAAACCATCATGGAAAAATATGGAATCTTTGTTGATCTTAAACAAAAAGTTAGTAACTTAACAGTAGGTCAGCAACAACGGGTTGAGATCCTAAAGGTTTTATTTCGTGATAGTAATATCCTCATCTTTGATGAACCCACTGCAGTTTTAAGTGATCTTGAAATTCAAAACTTTCTCAAGATTATTGCTAACTTTAAAAAGCTAGGAAAAACAATTGTTTTAATCTCTCATAAATTAAATGAAATTAAACAAGTTGCTGATACAGCTACTGTCTTAAGACTTGGCAAGGTAGTTGGTAGATTTGATGTTAAAACAACACCAGTTGATAAGATTGCGCTTTTAATGATGGGCAAAGAGTTAAAACAAACTAAAAACACCACAGATTTTGTTGCTAAAGATGAACCTGTTTTAAAAGTTCAAAACCTGAATTTGTTTCTCAATAAATCTTTAGCATACAAGTTCTTAGTGAGATGCAATAACATCCATAAAGCCCAACAAATTAAGAAAAATAAACCATTAAAAGACTTATGGATAATTAGTTTTTTAAATAAACTAACCACCAGTAACAAAACCCCTAAATTAGTAAAAGGCTTGATTAATAAGTTAGGACTTTCCTATCAAGAAAATACAGATGAAACCATTAGTTTTGCTATCCATAAGGGAGAAATTTTTGCTATTGCTGGGGTTGAGGGTAATGGTCAAAGTCAGCTTGTTAATTTAATTTGTGGAATTGAAAAAGCTGCTAGTAATAAGTTAATTTTTAACAATATTGATATCTCAAGATGA", "aa": "MEKVAFKMEHISKSFDNGKIKANVDVSLVVYENTVHTILGENGAGKSTLTSILFGLYKPDSGKIFIGEKQVNFKSSKDAVKHKIGMVHQHFKLIENYTVLDNIILGNESRFGFLPLINRKVSEAKIKTIMEKYGIFVDLKQKVSNLTVGQQQRVEILKVLFRDSNILIFDEPTAVLSDLEIQNFLKIIANFKKLGKTIVLISHKLNEIKQVADTATVLRLGKVVGRFDVKTTPVDKIALLMMGKELKQTKNTTDFVAKDEPVLKVQNLNLFLNKSLAYKFLVRCNNIHKAQQIKKNKPLKDLWIISFLNKLTTSNKTPKLVKGLINKLGLSYQENTDETISFAIHKGEIFAIAGVEGNGQSQLVNLICGIEKAASNKLIFNNIDISR", "aa_hexdigest": "74eb4a29017a7816f92618034db5a1ea", "start_type": "ATG", "rbs_motif": null, "genes": [ "nupO" ], "truncated": "3-prime", "pseudogene": { "start": 144966, "stop": 146432, "observations": { "mutation": [ 146127, 146262 ], "stop": [ 146127, 146262 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47365", "query_cov": 1.0, "subject_cov": 0.6861702127659575, "identity": 0.997, "score": 742.0, "evalue": 2.06e-267, "gene_start": 1, "gene_end": 387, "reference_start": 1, "reference_end": 387, "reference_sequence": "MEKVAFKMEHISKSFDNGKIKANVDVSLVVYENTVHTILGENGAGKSTLTSILFGLYKPDSGKIFIGEKQVNFKSSKDAVKHKIGMVHQHFKLIENYTVLDNIILGNESRFGFLPLINRKVSEAKIKTIMEKYGIFVDLKQKVSNLTVGQQQRVEILKVLFRDSNILIFDEPTAVLSDLEIQNFLKIIANFKKLGKTIVLISHKLNEIKQVADTATVLRLGKVVGSFDVKTTPVDKIALLMMGKELKQTKNTTDFVAKDEPVLKVQNLNLFLNKSLAYKFLVRCNNIHKAQQIKKNKPLKDLWIISFLNKLTTSNKTPKLVKGLINKLGLSYQENTDETISFAIHKGEIFAIAGVEGNGQSQLVNLICGIEKAASNKLIFNNIDISRWSIRKRINAGISFVLEDRHKYGLILDQTVRFNTVNNQINNRPFSSWNFLKPMEIALYSNTIIKKFDVRGSAEGSAVVRRLSGGNQQKLIIGREMTKQNDLLVLAQVTRGLDIGAIAFIHENILLAKANNKAILLVSYELDEILALADTVAVINKGRIVGMGKRDLMDRQSIGRLIMQ" }, "paralog": false, "identity": 0.9938524590163934, "score": 936.0, "evalue": 0.0, "description": "Internal stop codon at 146127, 146262. Nonsense mutation around 146127, 146262.", "psc": { "uniref90_id": "UniRef90_P47365", "gene": "NupO", "product": "Putative carbohydrate transport ATP-binding protein MG119", "uniref50_id": "UniRef50_P47365", "cog_id": "COG3845", "cog_category": "F", "kegg_orthology_id": "K23537", "go_ids": [ "GO:0005886", "GO:0005524", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47365", "UniRef:UniRef50_P47365", "COG:COG3845", "COG:F", "KEGG:K23537", "GO:0005886", "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47365", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47365" ], "product": "Putative carbohydrate transport ATP-binding protein MG119" } }, "id": "JPCNHPFPBM_299", "locus": "GCA000292505_00260" }, { "type": "cds", "sequence": "contig_1", "start": 146280, "stop": 146660, "strand": "+", "frame": 3, "gene": "mglA", "product": "Galactose/methyl galactoside import ATP-binding protein MglA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M965" ], "nt": "ATGGAGATTGCTCTTTATAGCAACACTATTATTAAAAAGTTTGATGTTAGGGGCAGTGCTGAGGGTAGTGCTGTTGTAAGAAGACTTTCAGGTGGTAATCAACAGAAACTAATTATTGGTCGAGAAATGACCAAACAAAATGACCTTTTGGTGTTAGCACAAGTAACCAGAGGCCTTGATATTGGTGCTATTGCTTTTATCCATGAAAACATCTTATTAGCTAAAGCTAATAATAAAGCTATCTTATTGGTTTCATATGAACTTGATGAGATCTTAGCACTTGCTGATACAGTGGCTGTTATCAATAAGGGGAGAATAGTTGGTATGGGAAAAAGAGATTTAATGGATCGCCAATCGATAGGTAGATTAATAATGCAATAA", "aa": "MEIALYSNTIIKKFDVRGSAEGSAVVRRLSGGNQQKLIIGREMTKQNDLLVLAQVTRGLDIGAIAFIHENILLAKANNKAILLVSYELDEILALADTVAVINKGRIVGMGKRDLMDRQSIGRLIMQ", "aa_hexdigest": "e869337ed1f0987f1d2aa27fd769dede", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A223M965", "query_cov": 0.9126984126984127, "subject_cov": 0.9274193548387096, "identity": 0.574, "score": 123.0, "evalue": 4.79e-34, "valid": false, "product": "Galactose/methyl galactoside import ATP-binding protein MglA", "uniref50_id": "UniRef50_A0A223M965" }, "pscc": { "uniref50_id": "UniRef50_A0A223M965", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M965" ], "product": "Galactose/methyl galactoside import ATP-binding protein MglA" }, "genes": [ "mglA" ], "id": "JPCNHPFPBM_300", "locus": "GCA000292505_00261" }, { "type": "cds", "sequence": "contig_1", "start": 146751, "stop": 147191, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAGAAGAAGTATCCTCTCTTCAGTGGTTTTGATAATCCTCTCTTTTCTTATATCGTTTTTACTGATTATTTCAATTCCTGGAGGTAGAGGTGCGAGCTTCTTTGCACTGTTTACTAAGTTATTTTTAGATAACACTAATACTGAAAATTTCTTAAGACAGATTGCTATTTATATCCTAGCTGGATTAGCATTTAGTTTCTGTATGAGTGTTGGTATTTTCAACATTGGTATCTCAGGGCAGATGATGGCTGGAGCCATCTTTGGGTTTTTAATGATTCTCAAGGTGTTTCCAAGTTCATTTCGACCTGGTTTTGGAGGTCAGATTATTACTGTATTATTGATGGTAATAGGTAGTGTTAGTGTGGCAGTTGTTGTTGCAACTTTAAAGATTTTCTTCAAGGTTAATGAAGTTGTAAGTGCAATTATGTTGAACTGA", "aa": "MQRRSILSSVVLIILSFLISFLLIISIPGGRGASFFALFTKLFLDNTNTENFLRQIAIYILAGLAFSFCMSVGIFNIGISGQMMAGAIFGFLMILKVFPSSFRPGFGGQIITVLLMVIGSVSVAVVVATLKIFFKVNEVVSAIMLN", "aa_hexdigest": "5029e9cca108206d566a5d1a98f2de81", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15812.016799999998, "isoelectric_point": 10.531829261779784 }, "id": "JPCNHPFPBM_301", "locus": "GCA000292505_00262" }, { "type": "cds", "sequence": "contig_1", "start": 147198, "stop": 147332, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTATTAGTGCTTATTTAGTAGAGACTTACATTAAAAATAATAGTGGGGGTACAGCCCAATTCTTTTCCTTACCACTCCCTGATGAATTTGCTTTATATAACTTCTCTCCTTTAACAAAAAAGTTTGGTTGA", "aa": "MLISAYLVETYIKNNSGGTAQFFSLPLPDEFALYNFSPLTKKFG", "aa_hexdigest": "ef71a9c424543b530de92ee293cb34f4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4934.6151, "isoelectric_point": 5.936628150939941 }, "id": "JPCNHPFPBM_302", "locus": "GCA000292505_00263" }, { "type": "cds", "sequence": "contig_1", "start": 147372, "stop": 147800, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCAGTAGTATTAAAATACACAGTTTTTGGACACAAATTAAAGTCAATTGGCAGTAGTGTATTTGGTTCTCAGGCAATGGGTTTTAATGTTAAAAAATACCAGTTCTTATCGTTTATTATCTCAGGAATTTTATCAGGACTATTAGCAACGGTTGTTTACACTGCATCAACTGAAAAAGTATTGACATTTAACAATGTTGGGGATAGTGCTATTTCAGCAGTACCAGCTACTGGTTTTGATGGGATTGCGATTGGTTTAATTGCTTTAAATAACCCCTTTAGGATTGTTATTGTTTCTGTTCTTATTGCTTTTGTTAACATTGGGGCAAGACCTGCTAATTTAAACCCTAGTACTGCTAGTTTAGTTTTAGGAATCATGATGTATTTTGCTGCACTTTATAACCTAATGGTTTACTTTAAACCATGA", "aa": "MAVVLKYTVFGHKLKSIGSSVFGSQAMGFNVKKYQFLSFIISGILSGLLATVVYTASTEKVLTFNNVGDSAISAVPATGFDGIAIGLIALNNPFRIVIVSVLIAFVNIGARPANLNPSTASLVLGIMMYFAALYNLMVYFKP", "aa_hexdigest": "b757aba318562ca10a18bd74a9e7ffa6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15091.734799999977, "isoelectric_point": 9.7943754196167 }, "id": "JPCNHPFPBM_303", "locus": "GCA000292505_00264" }, { "type": "cds", "sequence": "contig_1", "start": 148047, "stop": 148229, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGCAATCAAAAGTGGCAATATTAAACCTTCAACTACTTTTAAGTTGGAATTTGTTAATTTTAAACACCAACAGAAGTTTGTATTAAATTGGTTTAAAAATGAAAGTGAATCACTGCGTGATTTCCAATCACAGTTTGAGAGAATCAATAAGTTAGTGGAAAGGGAGTTTGTTAAGTAA", "aa": "MNAIKSGNIKPSTTFKLEFVNFKHQQKFVLNWFKNESESLRDFQSQFERINKLVEREFVK", "aa_hexdigest": "97948b4f1e5b7e13d4e01cde7bc1ae90", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7282.256099999995, "isoelectric_point": 9.77445468902588 }, "id": "JPCNHPFPBM_304", "locus": "GCA000292505_00265" }, { "type": "cds", "sequence": "contig_1", "start": 148232, "stop": 148429, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAGTTTAGCACAATTAGAAAGTTGGTTTTTTATCGCTCCAGCACTGCTTTTAGCAGTATTGAGTGGTTATCTTGCTGAACGCGTTGGGATCATTAATATTGCTATTAATGGTGGAATGGTGTTTGGTGGGTTATTTATGGCACTCTTATCGTATGGATTTACAAATAACTTAAACCAATCCGCTCCCTCCTGA", "aa": "MLSLAQLESWFFIAPALLLAVLSGYLAERVGIINIAINGGMVFGGLFMALLSYGFTNNLNQSAPS", "aa_hexdigest": "fd18b1108f04c3f653ff47333939cb4d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6908.0662999999995, "isoelectric_point": 4.529692649841309 }, "id": "JPCNHPFPBM_305", "locus": "GCA000292505_00266" }, { "type": "cds", "sequence": "contig_1", "start": 148529, "stop": 148951, "strand": "+", "frame": 2, "gene": "nupQ", "product": "putative protein MG121", "db_xrefs": [ "COG:COG1079", "COG:F", "GO:0005886", "GO:0022857", "KEGG:K23536", "SO:0001217", "UniRef:UniRef50_P47367", "UniRef:UniRef90_P47367" ], "nt": "GTGGGAACTGGGATTAACTTGTTGGCTAGCGGAATTACCCTTTTTATTAGTCAGAATGCTGCTAGTTTGTTTTCCGATACTACCTTAAGAGTAAGGTACTTATTTCCCATCCAAACTACTGTTAGTATAGAAGCAATTGGTGTGTTTGTTTTTAGTTTACTTCTGATTGGGTTTGTATGGTACTTGATGAGTTTTACTAAAACTGGGTTGAGATACCGTGCGGTAGGTGAGAATCCTAATGTCATTGATACCCAAGGGATTAGTGTTTACAAATACCAATGGATAGGCGCAATTTGTTCAATGATGGTAGCTGGATTGAGTGGTAGTTTGTTTGTTTTAAGTGTTTCTAACTTTCCCTTTAACAGCGGAGATGTAAATGGCTTGGGTTTTATTGCTATTGCCATTATGATTATCTCAATGTGA", "aa": "MGTGINLLASGITLFISQNAASLFSDTTLRVRYLFPIQTTVSIEAIGVFVFSLLLIGFVWYLMSFTKTGLRYRAVGENPNVIDTQGISVYKYQWIGAICSMMVAGLSGSLFVLSVSNFPFNSGDVNGLGFIAIAIMIISM", "aa_hexdigest": "eca65363cbce6200ca83e86a8fd98295", "start_type": "GTG", "rbs_motif": null, "genes": [ "nupQ" ], "truncated": "both", "pseudogene": { "start": 148232, "stop": 149152, "observations": { "mutation": [ 148427, 148949 ], "stop": [ 148427, 148949 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47367", "query_cov": 1.0, "subject_cov": 0.45751633986928103, "identity": 0.993, "score": 266.0, "evalue": 7.84e-88, "gene_start": 1, "gene_end": 140, "reference_start": 100, "reference_end": 239, "reference_sequence": "MLSLAQLESWFFIAPALLLAVLSGYLAERVGIINIAINGGMVFGGLFMALLSYGFTNNLNQSAPSWSLFITIPLSVLFSSVIGCLFALAAVKLRADHVIVGTGINLLASGITLFISQNAASLFSDTTLRVRYLFPIQTTVSIEAIGVFVFSLLLIGFVWYLMSFTKTGLRYRAVGENPNVIDTQGISVYKYQWIGAICSMMVAGLSGSLFVLSVSNFPFNSGDVNGLGFIAIAIMIISMWRIIPSIFIGLIFAYAYVFTNSQIGSNSNSYLLRTIPFIISLLVMLLFGFLNVAPKNIGKHFDKGLR" }, "paralog": false, "identity": 0.9934640522875817, "score": 583.0, "evalue": 4.65e-215, "description": "Internal stop codon at 148427, 148949. Nonsense mutation around 148427, 148949.", "psc": { "uniref90_id": "UniRef90_P47367", "gene": "NupQ", "product": "Uncharacterized protein MG121", "uniref50_id": "UniRef50_P47367", "cog_id": "COG1079", "cog_category": "F", "kegg_orthology_id": "K23536", "go_ids": [ "GO:0005886", "GO:0022857" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47367", "UniRef:UniRef50_P47367", "COG:COG1079", "COG:F", "KEGG:K23536", "GO:0005886", "GO:0022857" ] }, "pscc": { "uniref50_id": "UniRef50_P47367", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47367" ], "product": "Uncharacterized protein MG121" } }, "id": "JPCNHPFPBM_306", "locus": "GCA000292505_00267" }, { "type": "cds", "sequence": "contig_1", "start": 149192, "stop": 149353, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAAAAACCTGGTGGTGATTGAATCACCCAATAAAGTTAAAACATTAAAACAATATCTTCCTAGTGATGAATTTGAGATAGTCTCAACCGTTGGTCACATCAGAGAAATGGTGTATAAAAACTTTGGTTTTGATGAAAATACCTATACCCCTATCTGA", "aa": "MIKNLVVIESPNKVKTLKQYLPSDEFEIVSTVGHIREMVYKNFGFDENTYTPI", "aa_hexdigest": "70b39fdba60de8fd39e6d8f91219a05a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6177.061699999999, "isoelectric_point": 5.644021034240722 }, "id": "JPCNHPFPBM_307", "locus": "GCA000292505_00268" }, { "type": "cds", "sequence": "contig_1", "start": 149522, "stop": 150961, "strand": "+", "frame": 2, "gene": "topA", "product": "type I DNA topoisomerase", "db_xrefs": [ "COG:COG0551", "COG:L", "EC:5.6.2.1", "GO:0003677", "GO:0003917", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0006265", "GO:0046872", "KEGG:K03168", "SO:0001217", "UniRef:UniRef50_P47368", "UniRef:UniRef90_P47368" ], "nt": "TTGGATCAAAAAGATAAAGCTAAGTGCAAACGAATCACTTTCAATGAGATCACTAAAAAAGCAGTAGTAGATGCATTAAAACAACCGCGTAACATCGATCTTAACTGGGTTGAAAGTCAGTTTGCCCGCCAAATCCTTGACAGGATGATTGGTTTTAGATTATCAAGATTATTAAATAGTTATCTGCAAGCAAAGTCTGCAGGTAGAGTTCAATCAGTGGCTTTGCGCTTTCTTGAGGAAAGAGAAAAGGAGATAGCTAAGTTTGTTCCGCGTTTTTGGTGGACAGTTGATGTTTTATTAAACAAAGAAAATAACCAAAAAGTAGTTTGTGCAAACAAGTCTATTTCTTTGGTTTTAAGAGAAATTAACCCTGAATTAAGTGCTAGTTTAAAACTGGGTTTTGAAGCTGCTGAAAACGTATCAGGAATTGACTTTTTAAATGAAGCTTCAGCAACCAGATTTGCCAACCAACTGACTGGCGAATATGAAGTTTATTTTATTGATGAACCTAAGATTTACTATTCATCTCCAAACCCAGTTTATACCACCGCTTCACTTCAAAAGGATGCAATTAATAAGTTAGGATGGTCTTCCAAAAAAGTAACAATGGTGGCCCAAAGACTGTATGAAGGGATTAGTGTTAATGGGAAACAAACTGCATTAATTAGTTATCCAAGAACTGATTCAATTAGGATTTCAAACCAATTTCAATCAGAGTGTGAAAAGTACATTGAAAAGGAGTTTGGAAGTCATTATTTAGCTGATAAAAATAAGTTAAAAAGACATAAAAAGGATGAGAAAATCATCCAAGATGCCCATGAAGGGATCCATCCTACTTACATTACTATTACCCCCAATGATCTGAAAAACGGGGTGAAACGCGATGAGTTTCTCCTTTATCGTTTAATATGGATTAGAACAGTTGCCAGTTTAATGGCAGATGCTAAAACATCAAGAACTATTGTTCGTTTTATAAACCAAAAAAACAAGTTTTATACCTCTTCAAAATCACTTTTATTTGATGGTTATCAAAGGTTATATGAAGAGATTAAACCTAATACTAAAGATGAACTTTACATTGATCTTAGTAAGCTTAAAATTGGTGATAAATTTAGTTTTGAAAAGATCAGCGTTAATGAGCATAAAACCAACCCACCACCACGTTACACCCAAGCTAGTTTAATTGAAGAGCTTGAAAAATCTAACATCGGTCGTCCCTCTACTTATAACACTATGGCCAGTGTTAATCTAGAAAGGGGCTATGCTAACTTAGTGAACCGATTTTTTTATATCACTGAGCTTGGTGAAAAAGTTAATAATGAACTTTCCAAGCATTTTGGGAATGTAATTAATAAAGAATTTACCAAGAAGATGGAAAAATCTTTGGATGAAATTGCTGAAAACAAAGTAAACTATCAAGAATTTCTTAAGCAGTTTTGA", "aa": "MDQKDKAKCKRITFNEITKKAVVDALKQPRNIDLNWVESQFARQILDRMIGFRLSRLLNSYLQAKSAGRVQSVALRFLEEREKEIAKFVPRFWWTVDVLLNKENNQKVVCANKSISLVLREINPELSASLKLGFEAAENVSGIDFLNEASATRFANQLTGEYEVYFIDEPKIYYSSPNPVYTTASLQKDAINKLGWSSKKVTMVAQRLYEGISVNGKQTALISYPRTDSIRISNQFQSECEKYIEKEFGSHYLADKNKLKRHKKDEKIIQDAHEGIHPTYITITPNDLKNGVKRDEFLLYRLIWIRTVASLMADAKTSRTIVRFINQKNKFYTSSKSLLFDGYQRLYEEIKPNTKDELYIDLSKLKIGDKFSFEKISVNEHKTNPPPRYTQASLIEELEKSNIGRPSTYNTMASVNLERGYANLVNRFFYITELGEKVNNELSKHFGNVINKEFTKKMEKSLDEIAENKVNYQEFLKQF", "aa_hexdigest": "2b3c62eb1f5a482394998330af91f255", "start_type": "TTG", "rbs_motif": null, "genes": [ "topA" ], "truncated": "both", "pseudogene": { "start": 149222, "stop": 151252, "observations": { "mutation": [ 149351, 149360, 150959 ], "stop": [ 149351, 149360, 150959 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47368", "query_cov": 1.0, "subject_cov": 0.6755994358251057, "identity": 0.9940000000000001, "score": 932.0, "evalue": 0.0, "gene_start": 1, "gene_end": 479, "reference_start": 111, "reference_end": 589, "reference_sequence": "MIKNLVVIESPNKVKTLKQYLPSDEFEIVSTVGHIREMVYKNFGFDENTYTPIWEDWTKNKQKNPKQKHLLSKFEIIKSIKAKASDAQNIFLASDPDREGEAISWHVYDLLDQKDKAKCKRITFNEITKKAVVDALKQPRNIDLNWVESQFARQILDRMIGFRLSRLLNSYLQAKSAGRVQSVALRFLEEREKEIAKFVPRFWWTVDVLLNKENNQKVVCANKSIPLVLREINPELSASLKLDFEAAENVSGIDFLNEASATRFANQLTGEYEVYFIDEPKIYYSSPNPVYTTASLQKDAINKLGWSSKKVTMVAQRLYEGISVNGKQTALISYPRTDSIRISNQFQSECEKYIEKEFGSHYLADKNKLKRHKKDEKIIQDAHEGIHPTYITITPNDLKNGVKRDEFLLYRLIWIRTVASLMADAKTSRTIVRFINQKNKFYTSSKSLLFDGYQRLYEEIKPNTKDELYIDLSKLKIGDKFSFEKISVNEHKTNPPPRYTQASLIEELEKSNIGRPSTYNTMASVNLERGYANLVNRFFYITELGEKVNNELSKHFGNVINKEFTKKMEKSLDEIAENKVNYQEFLKQFWTNFKSDVKLAENSIQKVKKEKELVERDCPKCNQPLVYRYTKRGNEKFVGCSDFPKCKYSEFSNPKPKLTLETLDELCPECNNKLVKRRTKFNAKKTFIGCSNFPNCRFIKKDNAAEFKQ" }, "paralog": false, "identity": 0.9911242603550295, "score": 1274.0, "evalue": 0.0, "description": "Internal stop codon at 149351, 149360, 150959. Nonsense mutation around 149351, 149360, 150959.", "psc": { "uniref90_id": "UniRef90_P47368", "gene": "topA", "product": "type I DNA topoisomerase", "ec_ids": [ "5.6.2.1" ], "uniref50_id": "UniRef50_P47368", "cog_id": "COG0551", "cog_category": "L", "kegg_orthology_id": "K03168", "go_ids": [ "GO:0003677", "GO:0003917", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0006265", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47368", "EC:5.6.2.1", "UniRef:UniRef50_P47368", "COG:COG0551", "COG:L", "KEGG:K03168", "GO:0003677", "GO:0003917", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0006265", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47368", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47368" ], "product": "DNA topoisomerase 1" } }, "id": "JPCNHPFPBM_308", "locus": "GCA000292505_00269" }, { "type": "cds", "sequence": "contig_1", "start": 151010, "stop": 151321, "strand": "+", "frame": 2, "gene": null, "product": "topoisomerase DNA-binding C4 zinc finger domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED90A12" ], "nt": "GTGAAAAAGGAAAAAGAATTGGTTGAAAGAGATTGTCCTAAATGTAATCAACCGTTGGTATATCGTTACACCAAAAGAGGTAATGAGAAGTTTGTTGGTTGTAGTGATTTTCCTAAGTGTAAATACAGTGAGTTTAGTAATCCTAAACCAAAACTAACCTTGGAAACACTTGATGAATTGTGTCCTGAGTGTAACAATAAACTGGTTAAGAGGAGAACTAAATTTAACGCTAAAAAGACCTTTATAGGTTGCAGTAATTTCCCTAACTGCCGTTTTATCAAAAAGGATAATGCTGCTGAATTTAAACAATAA", "aa": "MKKEKELVERDCPKCNQPLVYRYTKRGNEKFVGCSDFPKCKYSEFSNPKPKLTLETLDELCPECNNKLVKRRTKFNAKKTFIGCSNFPNCRFIKKDNAAEFKQ", "aa_hexdigest": "40f7b4a6aae3db1a0e228e74a2cd6e8f", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001ED90A12", "query_cov": 0.8252427184466019, "subject_cov": 1.0, "identity": 0.8590000000000001, "score": 121.0, "evalue": 3.61e-34, "valid": false, "product": "topoisomerase DNA-binding C4 zinc finger domain-containing protein", "uniref50_id": "UniRef50_UPI001ED90A12" }, "pscc": { "uniref50_id": "UniRef50_UPI001ED90A12", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001ED90A12" ], "product": "topoisomerase DNA-binding C4 zinc finger domain-containing protein" }, "genes": [], "id": "JPCNHPFPBM_309", "locus": "GCA000292505_00270" }, { "type": "cds", "sequence": "contig_1", "start": 151299, "stop": 152291, "strand": "+", "frame": 3, "gene": null, "product": "MPN262 family protein", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_P47369", "UniRef:UniRef90_P47369" ], "nt": "ATGCTGCTGAATTTAAACAATAACAGCGTTCTTATTGTTGCGTTTGTAATTGTTTCTTTATTCTTTCTAATAATTGTTGGGTTTGCTTTAAATTTAGCAATTGCTTTTTCACTCCATTTAAAGCAGAATAAAAATAACAAAAAATACATCTTAAATGACCAACAGATCCAGTTAAGATTAACTGAAAAACAAGCCCAATTAACAACTTTACTTAACTTTTATCAACAAAAAATTGAAAGTGTAAACAGAGAAAAAAGTTGGTTAGAAAGTCAGTTACAGGTAATTGATAAAAAGGATTTAAAGCAAGCGCAAAAGTTAACTTTACATTTAAAAAAAGACCAAATATTAGCTCAACTTAATGAAAAGCTGATTCAAAAAAAAGTTGATCAGCCTTTAGTTAATGAACTACAGAAAACCAAACTTTCCTATCTTGAAAGGTTAGTTGATCAAAAGATTAAACTCAGTGAAAATAATTTCAAAAGTGCTTTTCTTAAAACGAAGGTAAAAGAGACAGCATTTAATATCTTTGCAGCTAAAAACAAGGTTAACTGGGAGTATTTTAAACAGGTGTGTGATGCTGATTGCACTTTAAAAAACTTAGAAGATGAAGTGGAAATTACTTTTTCTAATTGGAGTTATTTGAGAAGGATGCAAGCTCTATTAGCGTTTGAAAAACTAATTAGCAAAATCAAAACAGTCAAAATTAATGAATTGGTTATTAATGAAACTTTAGATGAAGTGAAAAACGAAATTAGCCAAACTGCTTTTCAAGCGGGTGAAAAAATAGTTAAGGAATTTCAGATAACTAATTTAAACGAGCAAATTACCAGACTAATTGGCTTGCAAAAATACTATTTTGGCACTGATCAGCTCAATCTCTTAGAACTCGCGGTATTAACAACCAAATTAGTAATTTTACTGAACAAAAAGTTCAAACTAGATTTAGATTTAGAACTTCTTAAAGCAGCTAGCTTGTTTAACTATTTAAAGTGA", "aa": "MLLNLNNNSVLIVAFVIVSLFFLIIVGFALNLAIAFSLHLKQNKNNKKYILNDQQIQLRLTEKQAQLTTLLNFYQQKIESVNREKSWLESQLQVIDKKDLKQAQKLTLHLKKDQILAQLNEKLIQKKVDQPLVNELQKTKLSYLERLVDQKIKLSENNFKSAFLKTKVKETAFNIFAAKNKVNWEYFKQVCDADCTLKNLEDEVEITFSNWSYLRRMQALLAFEKLISKIKTVKINELVINETLDEVKNEISQTAFQAGEKIVKEFQITNLNEQITRLIGLQKYYFGTDQLNLLELAVLTTKLVILLNKKFKLDLDLELLKAASLFNYLK", "aa_hexdigest": "c8ad9fb56cf947bede4fc5699690847a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 151299, "stop": 152594, "observations": { "mutation": [ 152289 ], "stop": [ 152289 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47369", "query_cov": 1.0, "subject_cov": 0.7006369426751592, "identity": 1.0, "score": 597.0, "evalue": 1.21e-212, "gene_start": 1, "gene_end": 330, "reference_start": 1, "reference_end": 330, "reference_sequence": "MLLNLNNNSVLIVAFVIVSLFFLIIVGFALNLAIAFSLHLKQNKNNKKYILNDQQIQLRLTEKQAQLTTLLNFYQQKIESVNREKSWLESQLQVIDKKDLKQAQKLTLHLKKDQILAQLNEKLIQKKVDQPLVNELQKTKLSYLERLVDQKIKLSENNFKSAFLKTKVKETAFNIFAAKNKVNWEYFKQVCDADCTLKNLEDEVEITFSNWSYLRRMQALLAFEKLISKIKTVKINELVINETLDEVKNEISQTAFQAGEKIVKEFQITNLNEQITRLIGLQKYYFGTDQLNLLELAVLTTKLVILLNKKFKLDLDLELLKAASLFNYLKWVDNNQFFQILNTKLNQLLISDQVIAIIQQQELSFYPDQYGMLINGVKTMIREHNTIDFEKLVFLNSTKLIDNFYLYDLNMIHAVEYNNCFYYFVSVKPFEIKSLAELDLFVVLLKTFLAKKQKQNPKAVKLFITTKILAI" }, "paralog": false, "identity": 0.9953596287703016, "score": 829.0, "evalue": 1.88e-306, "description": "Internal stop codon at 152289. Nonsense mutation around 152289.", "psc": { "uniref90_id": "UniRef90_P47369", "product": "MPN262 family protein", "uniref50_id": "UniRef50_P47369", "go_ids": [ "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47369", "UniRef:UniRef50_P47369", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47369", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47369" ], "product": "Uncharacterized protein MG123" } }, "id": "JPCNHPFPBM_310", "locus": "GCA000292505_00271" }, { "type": "cds", "sequence": "contig_1", "start": 152412, "stop": 152714, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATTAATGGAGTGAAAACAATGATTAGAGAGCATAACACCATTGATTTTGAAAAACTGGTTTTTCTCAACTCAACAAAACTTATTGATAATTTTTATCTCTATGATTTAAATATGATCCATGCAGTTGAATATAACAACTGCTTTTATTACTTTGTTAGTGTTAAACCCTTTGAGATTAAGTCATTAGCAGAACTTGATCTTTTTGTTGTTTTATTAAAAACTTTTTTAGCTAAAAAGCAAAAGCAAAATCCAAAAGCGGTTAAACTGTTTATCACTACCAAAATTTTAGCGATCTAA", "aa": "MLINGVKTMIREHNTIDFEKLVFLNSTKLIDNFYLYDLNMIHAVEYNNCFYYFVSVKPFEIKSLAELDLFVVLLKTFLAKKQKQNPKAVKLFITTKILAI", "aa_hexdigest": "2a9f4881a699bb72b0bf3c1e1bd5ef9b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11768.906399999985, "isoelectric_point": 9.212806129455569 }, "id": "JPCNHPFPBM_311", "locus": "GCA000292505_00272" }, { "type": "cds", "sequence": "contig_1", "start": 153075, "stop": 153764, "strand": "+", "frame": 3, "gene": "cof", "product": "Putative phosphatase MG125", "db_xrefs": [ "COG:COG0561", "COG:HR", "EC:3.1.3.-", "GO:0000287", "GO:0005829", "GO:0016791", "SO:0001217", "UniRef:UniRef50_P47371", "UniRef:UniRef90_P47371" ], "nt": "ATGATTGATCTGCTTGGTTTGGATCTGGATGGAACGTTATTATCTAAAACTAAAAAAATTAACAATCCATCAAAATTAGCATTAACTAATTTAATTGCTAAAAAACCAAGTTTAAAGGTGATGATTTTAACTGGTAGATCAGTTTTTTCTACTCTAAAACACGTTGAAAAGCTGAACAGTTTGTTTAAAAAACCAATTGTTGATTATTTTTGTTGTTATGGGGGTGCTAAACTTTATCAAATTGAAGCAAATAAGCCACAAGAAAGATACAAGTTTTGCTTGGAAAACAGTGTTGTTGAAACTACCTTTAGTATTATCAAAAAACACCGCGGATTATGTTTAGCTTACTTAGATAGTTATGTCTCTCCTTACCTTTGTTTAACTGGTAACAAGCTCCTTGGGTGGTTCACTAAATACTTTTGGTATAGAAAAAGGTGTGTGTTTTTTAACCAGAACCATTTAAAACAAGGTATTCTAAAGATTAGTGTTTACTTTTTAAGTGCAAAAAGGTGTAAAAAAGTTTATGAAATCTTAAAAAATACCTTTCAAGAAAAGGTTAATGTTTTAAGTTTTTCTAATAATTTAATTGAGATAACTCATCATGATGCTAATAAGGGTTATGCAATTGAATATATGGCCAAAAGAGAACAACTTTCACTTAATAGAATAGCAGTTATTGGTGATTCTTGA", "aa": "MIDLLGLDLDGTLLSKTKKINNPSKLALTNLIAKKPSLKVMILTGRSVFSTLKHVEKLNSLFKKPIVDYFCCYGGAKLYQIEANKPQERYKFCLENSVVETTFSIIKKHRGLCLAYLDSYVSPYLCLTGNKLLGWFTKYFWYRKRCVFFNQNHLKQGILKISVYFLSAKRCKKVYEILKNTFQEKVNVLSFSNNLIEITHHDANKGYAIEYMAKREQLSLNRIAVIGDS", "aa_hexdigest": "03660b15194b6c968d4e2ec17f6bdc0b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47371", "query_cov": 1.0, "subject_cov": 0.8035087719298246, "identity": 0.996, "score": 459.0, "evalue": 1.16e-162, "valid": true, "gene": "Cof", "product": "Putative phosphatase MG125", "ec_ids": [ "3.1.3.-" ], "uniref50_id": "UniRef50_P47371", "cog_id": "COG0561", "cog_category": "HR", "go_ids": [ "GO:0005829", "GO:0000287", "GO:0016791" ] }, "pscc": { "uniref50_id": "UniRef50_P47371", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47371" ], "product": "Putative phosphatase MG125" }, "genes": [ "cof" ], "id": "JPCNHPFPBM_312", "locus": "GCA000292505_00273" }, { "type": "cds", "sequence": "contig_1", "start": 153777, "stop": 153932, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCAAAAAAGCTAAATATTCCTTTGCAATGTCAAAATCCCCTTCCCAGTTAAAATTAATTGCTACCAATACCAGTAACAAAACCAACCGTTACCGCTTTAGTACCTTACTTAATTTAATTAGTGAAACAATCATTAATCAAAAAGCTGATTAG", "aa": "MFKKAKYSFAMSKSPSQLKLIATNTSNKTNRYRFSTLLNLISETIINQKAD", "aa_hexdigest": "586c7c2a6f62f259930e18d13f136901", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5843.731199999998, "isoelectric_point": 10.29278049468994 }, "id": "JPCNHPFPBM_313", "locus": "GCA000292505_00274" }, { "type": "cds", "sequence": "contig_1", "start": 153913, "stop": 154956, "strand": "-", "frame": 1, "gene": "trpS", "product": "tryptophan--tRNA ligase", "db_xrefs": [ "COG:COG0180", "COG:J", "EC:6.1.1.2", "GO:0000166", "GO:0004830", "GO:0005524", "GO:0005829", "GO:0006436", "RefSeq:WP_014893947.1", "SO:0001217", "UniParc:UPI00027B3541", "UniRef:UniRef100_UPI00027B3541", "UniRef:UniRef50_P75510", "UniRef:UniRef90_P47372" ], "nt": "ATGATAAAGCGCGCAATTACAGGGATTCAAGCTTCTGGAAGACAACACCTAGGTAACTTTCTTGGTGTAATGCAAGGTTTAAAACAACTCCAAAGTCAATACCAACTGTTTTTATTTGTTGCTGATCTTCATGCTATTACTGTTGATTTTGAACCAACAATGCTCAAAGATAACAACTTGCAACTTGTTAAAACTTTATTAGCACTAGGACTTGATTATGGAAAAGTGAACTTATTTTTACAAAGTGATCTGATGGAACATACCATGTTAGGTTATCTAATGCTGACACAAAGTAATCTAGGTGAATTACAAAGAATGACCCAATTTAAAACAAAGAAATTAGCGCAAAAAAGAAATAGTAATAACACCATTACTATCCCAACTGGTTTGTTAACTTACCCAGTGTTAATGGCTGCTGATATCTTGCTTTATCAACCTGATATTGTTCCAGTTGGTAATGATCAGAAGCAGCACTTGGAATTAACCAATGATTTAGCTAAACGTATAGCAAAAAAATTTAAGTTAAAACTGAAATTACCTGTATTTATAGAAAACAAAGATACCAACAGGATCATGGATCTATCAAATCCTTTAAAGAAGATGTCCAAATCAAATCCTGATCAAAATGGTGTTATCTATCTGGATGATAGTAAAGAAACAATCATCAAAAAAGTGCGCAAAGCCACAACTGATAGTTTTAATAAGATTCGTTTTGCTAAAAAAACCCAACCTGGTGTTACTAATTTACTTGTTATTTTAACTGCACTTTTAAAAGAAGAAGTTAACCATAATTTAAGTAAAAAAATCGGCTCTGATCTTGTTAAATATTATCAGAATAAAAGTTATTTAGATTTAAAGAATGACCTCAGTAGTGCTGTTATTAATGTCATAGAATCACTTAAATTTAAAAAAGCACAAATTACTGATGAAATGGTATTAAAAGTCCTAAATGATGGTAAAAACCAAGCTAAAAAAGTTGCTGATGAAACATTAAAAATGTTTTATAAAGCATTTGGTTTAACATCTAATCAGCTTTTTGATTAA", "aa": "MIKRAITGIQASGRQHLGNFLGVMQGLKQLQSQYQLFLFVADLHAITVDFEPTMLKDNNLQLVKTLLALGLDYGKVNLFLQSDLMEHTMLGYLMLTQSNLGELQRMTQFKTKKLAQKRNSNNTITIPTGLLTYPVLMAADILLYQPDIVPVGNDQKQHLELTNDLAKRIAKKFKLKLKLPVFIENKDTNRIMDLSNPLKKMSKSNPDQNGVIYLDDSKETIIKKVRKATTDSFNKIRFAKKTQPGVTNLLVILTALLKEEVNHNLSKKIGSDLVKYYQNKSYLDLKNDLSSAVINVIESLKFKKAQITDEMVLKVLNDGKNQAKKVADETLKMFYKAFGLTSNQLFD", "aa_hexdigest": "2cfd4403f99558215ca1a88b8e66bd60", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3541", "ncbi_nrp_id": "WP_014893947.1", "uniref100_id": "UniRef100_UPI00027B3541", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3541", "RefSeq:WP_014893947.1", "UniRef:UniRef100_UPI00027B3541" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3541", "uniref90_id": "UniRef90_P47372" }, "psc": { "uniref90_id": "UniRef90_P47372", "gene": "trpS", "product": "tryptophan--tRNA ligase", "ec_ids": [ "6.1.1.2" ], "uniref50_id": "UniRef50_P75510", "cog_id": "COG0180", "cog_category": "J", "go_ids": [ "GO:0000166", "GO:0004830", "GO:0005524", "GO:0005829", "GO:0006436" ] }, "pscc": { "uniref50_id": "UniRef50_P75510", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75510" ], "product": "Tryptophan--tRNA ligase" }, "genes": [ "trpS" ], "id": "JPCNHPFPBM_314", "locus": "GCA000292505_00275" }, { "type": "cds", "sequence": "contig_1", "start": 154992, "stop": 155429, "strand": "+", "frame": 3, "gene": "arsC", "product": "putative protein MG127", "db_xrefs": [ "COG:COG1393", "COG:P", "GO:0016491", "KEGG:K16509", "RefSeq:WP_010869345.1", "SO:0001217", "UniParc:UPI0000139302", "UniRef:UniRef100_P47373", "UniRef:UniRef50_P75509", "UniRef:UniRef90_P47373" ], "nt": "ATGAAAAAAGCGGGTAAAAAAAATAGTGATGCAGGTAAGACTTTTATCTTAATCTCATCATCCTGTTCATCCTGTCAGAAAGCCATTGAGTTTTTTGATCAAAACAAAATTAGCTATGTTGTTGAGAATTTTTATAAAAAACCAATTAGTGATAAGCGTTTTAAAGATATTTTAAGTCTTAGTGAGGATGGTACTGAAAGTTTGTTTTCCAAACGTGCTGATCAGATTAAAGCAACTAACAGTGTTAGTGTTGAAGAGTTGAGTATCAGTGAATTAATTAAGCTAGTTAGAGAACGTCCTTCTTTACTAAGAAGGCCTATTATCATCCAATATAATTCTTCAGGAATTCCTAAAAGGATGCGAATTGGTTATAACTCATCTGAAATTAAGGTCTTTGAGCGTAAACTAATAGAACCAAAGCCTATAATACAACAATAA", "aa": "MKKAGKKNSDAGKTFILISSSCSSCQKAIEFFDQNKISYVVENFYKKPISDKRFKDILSLSEDGTESLFSKRADQIKATNSVSVEELSISELIKLVRERPSLLRRPIIIQYNSSGIPKRMRIGYNSSEIKVFERKLIEPKPIIQQ", "aa_hexdigest": "40e8ac7b4528c13aeb66ae30ddcdf3a3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139302", "ncbi_nrp_id": "WP_010869345.1", "uniref100_id": "UniRef100_P47373", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139302", "RefSeq:WP_010869345.1", "UniRef:UniRef100_P47373" ] }, "ips": { "uniref100_id": "UniRef100_P47373", "uniref90_id": "UniRef90_P47373" }, "psc": { "uniref90_id": "UniRef90_P47373", "gene": "ArsC", "product": "Uncharacterized protein MG127", "uniref50_id": "UniRef50_P75509", "cog_id": "COG1393", "cog_category": "P", "kegg_orthology_id": "K16509", "go_ids": [ "GO:0016491" ] }, "pscc": { "uniref50_id": "UniRef50_P75509", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75509" ], "product": "Uncharacterized protein MG127 homolog" }, "genes": [ "arsC" ], "id": "JPCNHPFPBM_315", "locus": "GCA000292505_00276" }, { "type": "cds", "sequence": "contig_1", "start": 155437, "stop": 156216, "strand": "+", "frame": 1, "gene": "nadK", "product": "NAD(+) kinase", "db_xrefs": [ "COG:COG0061", "COG:H", "EC:2.7.1.23", "GO:0003951", "GO:0005524", "GO:0005737", "GO:0006741", "GO:0019674", "GO:0046872", "GO:0051287", "KEGG:K00858", "RefSeq:WP_014894158.1", "SO:0001217", "UniParc:UPI00027B3542", "UniRef:UniRef100_UPI00027B3542", "UniRef:UniRef50_P75508", "UniRef:UniRef90_P47374" ], "nt": "ATGAAATACAAAATTTTTGCTTCCACAACGCCCCAAACTGAACCTGTTCTCAATAAGTTAAGAACAGTTTTAAAAACTTGGCAAGCGGTTGAGAATGGTTATGAATATGTGTTTGTTTTAGGTGGGGATGGTTTTTTTGTTTCCACACTTGCTAACTATAACTGTGATAGTTGTAAGGTAGTTGGTATCAATACTGGCCACCTTGGTTTTTACACTTCTTTTAATGGAGATGATCTTGATGAAAATTTCATTTCAAAACTAACTAGCTTTGAGTTTAGAAAGATTAATTTACTGGAAGTGAAAACTGAAAACCACAGTTTCTTAGTTTTAAATGAACTTGCAGTTTACACCAACACTGCATATCCAATTAACATCTTTATTGATGATAACCCCTGGGAATCATACCGCGGTTCAGGGTTGCTAATTGGCCCAAGAACAGGCTCAACTGCCCTAGCAAAATCCGCTAAGGGAGCGGTTATTTTTCCAAATGTTGATGTTGTTCAAATTATTGAACTAAACCCCTTATTACATCCCAACCAAATCACAATTCAATCTCCGATTATTCTGCCAATGCAAACCAAGGTTGAATTTAGGATCAAAAAGGCTTTTAAAGCAGAGCAATTTCCCAATTTTTATGCAGATGGAATCAAGCTTGATTTAAAAAATGAAGATACCAGTATTAGCTTTCAGTTGGTTTTATCAAGATCAATGTTTCACGCTTCTTTAAAAACTAAGGATTTTATTGATAAACTAAAGTCAACTTTTATTAAGCAAAGCTAA", "aa": "MKYKIFASTTPQTEPVLNKLRTVLKTWQAVENGYEYVFVLGGDGFFVSTLANYNCDSCKVVGINTGHLGFYTSFNGDDLDENFISKLTSFEFRKINLLEVKTENHSFLVLNELAVYTNTAYPINIFIDDNPWESYRGSGLLIGPRTGSTALAKSAKGAVIFPNVDVVQIIELNPLLHPNQITIQSPIILPMQTKVEFRIKKAFKAEQFPNFYADGIKLDLKNEDTSISFQLVLSRSMFHASLKTKDFIDKLKSTFIKQS", "aa_hexdigest": "b6a04b8198a2fd4c1038b349981c046c", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3542", "ncbi_nrp_id": "WP_014894158.1", "uniref100_id": "UniRef100_UPI00027B3542", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3542", "RefSeq:WP_014894158.1", "UniRef:UniRef100_UPI00027B3542" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3542", "uniref90_id": "UniRef90_P47374" }, "psc": { "uniref90_id": "UniRef90_P47374", "gene": "nadK", "product": "NAD(+) kinase", "ec_ids": [ "2.7.1.23" ], "uniref50_id": "UniRef50_P75508", "cog_id": "COG0061", "cog_category": "H", "kegg_orthology_id": "K00858", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0046872", "GO:0051287", "GO:0003951", "GO:0019674", "GO:0006741" ] }, "pscc": { "uniref50_id": "UniRef50_P75508", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75508" ], "product": "NAD kinase" }, "genes": [ "nadK" ], "id": "JPCNHPFPBM_316", "locus": "GCA000292505_00277" }, { "type": "cds", "sequence": "contig_1", "start": 156234, "stop": 156569, "strand": "+", "frame": 3, "gene": "ptsG2", "product": "Putative phosphotransferase enzyme IIB component MG129", "db_xrefs": [ "COG:COG1264", "COG:G", "EC:2.7.1.-", "GO:0008982", "GO:0009401", "GO:0016020", "SO:0001217", "UniRef:UniRef50_P75507", "UniRef:UniRef90_Q49397" ], "nt": "TTGGGTTATATTTTTAGTTTTGGTTTACTCTATCTTTGGATAGTAAAAAAATCAAAACAAATTGCTCAACAACCCAACACAAAACTGGTTGAATCAACTTCTATTCCTTTTAAAGTTAAGGACTTTGTTAGTGCTTGTGGTGGTAAGAAAAACTTTGTTAATATAAAAACAACACCAACTCAATTAATAGTTACTTTTAAAGATGTTAACTCAGTGAGCTTAACAAAGCTTAATGCGCTTAATATCAAAGGAATTAACAAAAACCAAAACCAATTTCGTTTTGTACTTGGTAACTTTGTAAATGAATTGAAAAAAAAGATAGAAGATGAACAATAA", "aa": "MGYIFSFGLLYLWIVKKSKQIAQQPNTKLVESTSIPFKVKDFVSACGGKKNFVNIKTTPTQLIVTFKDVNSVSLTKLNALNIKGINKNQNQFRFVLGNFVNELKKKIEDEQ", "aa_hexdigest": "0f26b1eea215da6edac1f2fc5c3e7254", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q49397", "query_cov": 1.0, "subject_cov": 0.9487179487179487, "identity": 0.982, "score": 214.0, "evalue": 2.41e-70, "valid": true, "gene": "PtsG2", "product": "Putative phosphotransferase enzyme IIB component MG129", "ec_ids": [ "2.7.1.-" ], "uniref50_id": "UniRef50_P75507", "cog_id": "COG1264", "cog_category": "G", "go_ids": [ "GO:0016020", "GO:0008982", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P75507", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75507" ], "product": "Putative phosphotransferase enzyme IIB component MPN_268" }, "genes": [ "ptsG2" ], "id": "JPCNHPFPBM_317", "locus": "GCA000292505_00278" }, { "type": "cds", "sequence": "contig_1", "start": 156559, "stop": 157851, "strand": "+", "frame": 1, "gene": "rny", "product": "Ribonuclease Y", "db_xrefs": [ "COG:COG1418", "COG:JR", "EC:3.1.-.-", "GO:0003723", "GO:0004521", "GO:0005886", "GO:0006402", "SO:0001217", "UniRef:UniRef50_P47376", "UniRef:UniRef90_P47376" ], "nt": "ATGAACAATAACATTACTAACAGTATTGTCCAATTGTTTTTCAACACTTCTCTCTTTGCTTTTCTGTTTTTAATCATTATTGCGTTTAATTTATGCTTGTTTGCCTATCTTTATTTTCAGTACCGAATTTATAAGAAAAACCCTAAAAAAGCTAACAACTTTAAAGCGAATGAATATGAAAAAATTAAGCTATTAAAAAACCAAAATTTCACTGAAAGTAATAAATTAATTGCAACAACTAATGAGTTAAATGAACTTACTAGTCAACTAGATAATATCTTGGTTAGGATTATCAACAAACCACTAGCAAAGTTAGTTAATGATTTTTTAGATGAACAGATTAAACAGATAGTTAAGCTAGATAAAAACAGTTCTGATTTTCACTCAGAAAGTGATAATCTCCCTTTTTATACCAAACTCTTTAATGATTTTCACTTTGGTGTTGATAAATTAATAAACATTAACATAAAAAACCCTCTTTATAACTGGGTTTATAGCCCCAGTTTTTTAATTAGTGAAAGTGATTTTCGCAAGCTTAATGGTATTAGTGGTATCAATAAAAAGCTTTTTGTTGAAAAACTTAGAATTGAAGACATTGTGTTTACAGATCTCAACAAAAAGTATGAAGTTAATGTCTTGACAGAAAGTCCTATTAAAGCACAAAAAACAGTGTTAACTGTGCGTAATATCCTGATGAACGATTATGTTGATAATGAAAGGATTGAATCATATGTCCAACAAGCTAACTTCTTTTTTACTGAGCACTGTAAAAAGATCGGTAAAGAGATCTTAGAATCACTTAATATTTTTATCTCAAGTAGTTCACTACACCGTCATTTTGGCTTTTTAGCATTTCGCTATTCATTTGGACAAAATGTCTTATCCCATAGCCTTGAAACTGCATTTTTAACTGCCCACTTAGCAGCTTTAATAGAACTTGATAGTGAACTGAGTTTAAAGTGTGGATTGCTCCATGATATTGGTAAATCTAATGATGATAATGGTAAAGAGAGCCATACGATTACAGGCGCTAAATTCGCTGAGCAATTTCAACTACCTGATGACATTAAATACACAATTGCTAACCACCACAATAAACATATTGACAATACTTATTGTCGTTTAACACAAATTGCTGATAAACTATCTGCTGCTAGAATTGGTGCTAGAAGTGATAGTTCGCTTCTTTTTAAACAACTAAAAGATGAGTTGAAAAAGATTGTTGATAAAACTATTAATAATTTTCATACAACGATCTTACTAGGTCAAAGTGGTAGAAGGTTAATGATTTGA", "aa": "MNNNITNSIVQLFFNTSLFAFLFLIIIAFNLCLFAYLYFQYRIYKKNPKKANNFKANEYEKIKLLKNQNFTESNKLIATTNELNELTSQLDNILVRIINKPLAKLVNDFLDEQIKQIVKLDKNSSDFHSESDNLPFYTKLFNDFHFGVDKLININIKNPLYNWVYSPSFLISESDFRKLNGISGINKKLFVEKLRIEDIVFTDLNKKYEVNVLTESPIKAQKTVLTVRNILMNDYVDNERIESYVQQANFFFTEHCKKIGKEILESLNIFISSSSLHRHFGFLAFRYSFGQNVLSHSLETAFLTAHLAALIELDSELSLKCGLLHDIGKSNDDNGKESHTITGAKFAEQFQLPDDIKYTIANHHNKHIDNTYCRLTQIADKLSAARIGARSDSSLLFKQLKDELKKIVDKTINNFHTTILLGQSGRRLMI", "aa_hexdigest": "5dc2f5f20686ea15a53deb9fa41fe379", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47376", "query_cov": 1.0, "subject_cov": 0.8884297520661157, "identity": 0.991, "score": 827.0, "evalue": 1.9e-301, "valid": true, "gene": "rny", "product": "Ribonuclease Y", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P47376", "cog_id": "COG1418", "cog_category": "JR", "go_ids": [ "GO:0005886", "GO:0003723", "GO:0004521", "GO:0006402" ] }, "pscc": { "uniref50_id": "UniRef50_P47376", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47376" ], "product": "Ribonuclease Y" }, "genes": [ "rny" ], "id": "JPCNHPFPBM_318", "locus": "GCA000292505_00279" }, { "type": "cds", "sequence": "contig_1", "start": 157903, "stop": 158013, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGAAAAGATTAAAGCTGAAATTGCTAAGAATTCAATTACAAATCACTTCCCTATTAAAGTTGTAATTAGATATAACTTTGAACATAGTTTCAACACCAAAAGCTAA", "aa": "MVEKIKAEIAKNSITNHFPIKVVIRYNFEHSFNTKS", "aa_hexdigest": "5711583dc453818723a3f5b9a9c8542d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4234.875800000001, "isoelectric_point": 9.698124122619632 }, "id": "JPCNHPFPBM_319", "locus": "GCA000292505_00280" }, { "type": "cds", "sequence": "contig_1", "start": 158016, "stop": 158303, "strand": "+", "frame": 3, "gene": null, "product": "MPN270 family protein", "db_xrefs": [ "RefSeq:WP_014894161.1", "SO:0001217", "UniParc:UPI00027B3545", "UniRef:UniRef100_UPI00027B3545", "UniRef:UniRef50_Q9EXD1", "UniRef:UniRef90_UPI00027B3AA2" ], "nt": "ATGCAATACAGCGCTTTAATACCACTTTTTATATTACTAATTAGCTTAGTATTATTTTGTTTCAGCTTTAGAAAAAATCAAAGTGAAAATCAGATAGTAAAGATCTTATTTTTTGCTTATTGCATCGATTTTTTAGCTTTAATTCTTGCTGTAATGTTGCTCACTTTTTTAAGTCATGGGTTGTTAAGTTTAGCGATTTTAATCCCTGTCTTAGTTTTTTCAATAATAATGTTTTTTGTTATGGTTATTAGTCATTATCCCCTTATGAAAAGACTATTTGGAAATTAA", "aa": "MQYSALIPLFILLISLVLFCFSFRKNQSENQIVKILFFAYCIDFLALILAVMLLTFLSHGLLSLAILIPVLVFSIIMFFVMVISHYPLMKRLFGN", "aa_hexdigest": "5313ef91cfd34330cb74841903839d62", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3545", "ncbi_nrp_id": "WP_014894161.1", "uniref100_id": "UniRef100_UPI00027B3545", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3545", "RefSeq:WP_014894161.1", "UniRef:UniRef100_UPI00027B3545" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3545", "uniref90_id": "UniRef90_UPI00027B3AA2" }, "psc": { "uniref90_id": "UniRef90_UPI00027B3AA2", "product": "MPN270 family protein", "uniref50_id": "UniRef50_Q9EXD1" }, "pscc": { "uniref50_id": "UniRef50_Q9EXD1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9EXD1" ], "product": "Uncharacterized protein MG131 homolog" }, "genes": [], "id": "JPCNHPFPBM_320", "locus": "GCA000292505_00281" }, { "type": "cds", "sequence": "contig_1", "start": 158317, "stop": 158598, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_014894579.1", "SO:0001217", "UniParc:UPI00027B3546", "UniRef:UniRef100_UPI00027B3546", "UniRef:UniRef50_UPI00027B3546", "UniRef:UniRef90_UPI00027B3546" ], "nt": "ATGGAGTTGAAAACCCCTAATTTTAAGCTAATTGATGAAAAGATTGCTGAATTTAATAAGAGCAATGAAAACCTGATTGTAAAACTACTTCAAAAAGAAAAGGAATTTGCCACAAACCAAGTTACTATTCAGTTTGATACTCAGTCAAAAAAGTCAGAAGAAGTGAAAAAACCTAGTAAAAAAATACTGAAAAATTATCACTTTCTAACAGTAGTTATGGCAGTTAATTTGCTAATAACTGTTGGTTTGATAGTTGGTTTAATTTTTGCTTTCAGATATTAG", "aa": "MELKTPNFKLIDEKIAEFNKSNENLIVKLLQKEKEFATNQVTIQFDTQSKKSEEVKKPSKKILKNYHFLTVVMAVNLLITVGLIVGLIFAFRY", "aa_hexdigest": "9902819cd6e11ed9730a20e2170c00a4", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3546", "ncbi_nrp_id": "WP_014894579.1", "uniref100_id": "UniRef100_UPI00027B3546", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3546", "RefSeq:WP_014894579.1", "UniRef:UniRef100_UPI00027B3546" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3546", "uniref90_id": "UniRef90_UPI00027B3546" }, "psc": { "uniref90_id": "UniRef90_UPI00027B3546", "uniref50_id": "UniRef50_UPI00027B3546" }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3546", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3546" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10789.648899999993, "isoelectric_point": 9.52289905548096 }, "id": "JPCNHPFPBM_321", "locus": "GCA000292505_00282" }, { "type": "cds", "sequence": "contig_1", "start": 158575, "stop": 159000, "strand": "-", "frame": 1, "gene": "hinT", "product": "putative HIT-like protein MG132", "db_xrefs": [ "COG:COG0537", "COG:FR", "GO:0003824", "GO:0009117", "RefSeq:WP_014894580.1", "SO:0001217", "UniParc:UPI00027B3547", "UniRef:UniRef100_UPI00027B3547", "UniRef:UniRef50_P47378", "UniRef:UniRef90_P47378" ], "nt": "ATGGAAAAAAACACCACAAGTAGTTGTATCTTTTGTGATATTGTTCAAGGTTCAATTACATCCTATAAAATTGGAGAAAATGAGCATGCCATTGCTTTTTTAGATGCTTTTCCTGTAGCTGATGGTCATACTTTAGTGATCCCTAAAAAACATGCAGCTAATTTTTCTTCAACTGATCAAAAGGAGTTACAAGCAGTTAGTTTATTAGCAAAACAAATCGCTTTAAAACTAAAGATGACACTAAAACCATCTGGTTTAAACTATATTTCTAATGAAGGGGCAATTGCAGGTCAAGTGGTTTTTCATTTTCACTTACACATAGTACCCAAATACGAAACTGGTAAAGGGTTTGGCTATAATGTCAATAAAACCAACAAACGTTCCTTAGAAGAAAACTACCAACTAATATCTGAAAGCAAAAATTAA", "aa": "MEKNTTSSCIFCDIVQGSITSYKIGENEHAIAFLDAFPVADGHTLVIPKKHAANFSSTDQKELQAVSLLAKQIALKLKMTLKPSGLNYISNEGAIAGQVVFHFHLHIVPKYETGKGFGYNVNKTNKRSLEENYQLISESKN", "aa_hexdigest": "37f4df0da91633c2bc260a6e36030d94", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3547", "ncbi_nrp_id": "WP_014894580.1", "uniref100_id": "UniRef100_UPI00027B3547", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3547", "RefSeq:WP_014894580.1", "UniRef:UniRef100_UPI00027B3547" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3547", "uniref90_id": "UniRef90_P47378" }, "psc": { "uniref90_id": "UniRef90_P47378", "gene": "HinT", "product": "Uncharacterized HIT-like protein MG132", "uniref50_id": "UniRef50_P47378", "cog_id": "COG0537", "cog_category": "FR", "go_ids": [ "GO:0003824", "GO:0009117" ] }, "pscc": { "uniref50_id": "UniRef50_P47378", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47378" ], "product": "Uncharacterized HIT-like protein MG132" }, "genes": [ "hinT" ], "id": "JPCNHPFPBM_322", "locus": "GCA000292505_00283" }, { "type": "cds", "sequence": "contig_1", "start": 159068, "stop": 159346, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTCTTTTATTAATGACTGGAATGCTTTTTCAGAGCTATTACTCCATGTTATCCATCCGTTGTTTTACTTTATTTATGTTGGATTACTTTTTAAATTTTATCAAACCGCCAAGGAATTTGCAATCCTTTCTTTTAAAAGCAGGCATTTATCCCAGTATCTATGCTTTTTATCTCCAAACTATTCCATTTTTAAATGTTTGGGATAATGGTGAAAACTCCTATTCAGTGTATGGATTTTTTACGCAAACTAAATATAACAGTTATGTTTGGATTTGA", "aa": "MVLLLMTGMLFQSYYSMLSIRCFTLFMLDYFLNFIKPPRNLQSFLLKAGIYPSIYAFYLQTIPFLNVWDNGENSYSVYGFFTQTKYNSYVWI", "aa_hexdigest": "a374d9d3ebe44ce0c879827d5ea05151", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11013.815599999993, "isoelectric_point": 8.73264560699463 }, "id": "JPCNHPFPBM_323", "locus": "GCA000292505_00284" }, { "type": "cds", "sequence": "contig_1", "start": 159540, "stop": 159656, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGATTACTGCACTGATTAGTGTTGCTAACAGTGGTCAAATGAGCCTGATTTGGTTTACTAATTTTGATACTTTTACTTACCAAAGTAATAGCCTTGCAATCTTTTTAGTATGA", "aa": "MVITALISVANSGQMSLIWFTNFDTFTYQSNSLAIFLV", "aa_hexdigest": "25df52711d8cc99d50c5732025be87d2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4244.880099999999, "isoelectric_point": 4.0500284194946286 }, "id": "JPCNHPFPBM_324", "locus": "GCA000292505_00285" }, { "type": "cds", "sequence": "contig_1", "start": 159784, "stop": 160086, "strand": "+", "frame": 1, "gene": "ybaB", "product": "YbaB/EbfC family nucleoid-associated protein", "db_xrefs": [ "COG:COG0718", "COG:K", "GO:0003677", "KEGG:K09747", "RefSeq:WP_010869347.1", "SO:0001217", "UniParc:UPI000013932E", "UniRef:UniRef100_P47380", "UniRef:UniRef50_P47380", "UniRef:UniRef90_P47380" ], "nt": "ATGAGTTTTAAAAAGATTGCTGAAATGATGCGTCAAGCAGAACGAGAAACTAAGAAAAAAACATTAGCATTTGAACAACAAGCCTTTGAATACAACTATAAAAATGGTGCGATTAAGATCACTATTTTAGGTGATCTTACACTTAAATCAATTAACATCGATCCTGTTTTGATTGATGCAAGCGACAAAGTTATTCTAGAGGAGATGATTATAGAAGCTACTAATGAAGCGGTTAGTGATGTGAAAACTAAGTATGATAACTTAGTTGAGAAAACTATGCCAAAAGTTCCAGGTCTTTTCTAA", "aa": "MSFKKIAEMMRQAERETKKKTLAFEQQAFEYNYKNGAIKITILGDLTLKSINIDPVLIDASDKVILEEMIIEATNEAVSDVKTKYDNLVEKTMPKVPGLF", "aa_hexdigest": "c413e76280bd3195594ee858ba01ca99", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013932E", "ncbi_nrp_id": "WP_010869347.1", "uniref100_id": "UniRef100_P47380", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013932E", "RefSeq:WP_010869347.1", "UniRef:UniRef100_P47380" ] }, "ips": { "uniref100_id": "UniRef100_P47380", "uniref90_id": "UniRef90_P47380" }, "psc": { "uniref90_id": "UniRef90_P47380", "gene": "YbaB", "product": "YbaB/EbfC family nucleoid-associated protein", "uniref50_id": "UniRef50_P47380", "cog_id": "COG0718", "cog_category": "K", "kegg_orthology_id": "K09747", "go_ids": [ "GO:0003677" ] }, "pscc": { "uniref50_id": "UniRef50_P47380", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47380" ], "product": "Uncharacterized protein MG134" }, "genes": [ "ybaB" ], "id": "JPCNHPFPBM_325", "locus": "GCA000292505_00286" }, { "type": "cds", "sequence": "contig_1", "start": 160058, "stop": 160249, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCGACCTTTCTTAACTTAAGCAATAGTGATCCACAACTTGTGTTTATTGTGGGCTTTAGTTTAAGTTATGTTGCGATTTTACTAATGACACTCTTTGCTAATATCCCTACATTTATGGAAATGAAAAAAGATAAGATCTTTATTAAAAAGGAAAATCTAATTAGAAAAGACCTGGAACTTTTGGCATAG", "aa": "MATFLNLSNSDPQLVFIVGFSLSYVAILLMTLFANIPTFMEMKKDKIFIKKENLIRKDLELLA", "aa_hexdigest": "a115bad6ad2a63a293ff095ede57b6f9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7239.664999999997, "isoelectric_point": 8.064559745788571 }, "id": "JPCNHPFPBM_326", "locus": "GCA000292505_00287" }, { "type": "cds", "sequence": "contig_1", "start": 160271, "stop": 160900, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MG135", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47381", "UniRef:UniRef90_P47381" ], "nt": "ATGCAGACACTTAACTACCTTGTCATCATCCTTATTATTGCTGGTGTAATTTCTGTTTTAGCTTTTACTCCTTTAGTTCGAAAACTAAAGATTAGATTCTATCTAATTCAAGCACTAGCCATTGTCCTGTTTGTCTATGTTTTTTTTGGTAGGCAGATTATTTATTTATTCCCTGATGTTTATGGACAAAATTCCCAATCTTCACAAACCTTAAATTCACTACGACTAAGTCGGATATTTTTATTAGATCTTTGTCCATTTTTTGCTGTTATTGCGCCAGTGTTTGTCTTTTTAAAGCAAAAAAAGATTAGTGGGGTGTTAGCTGTTTTTGGGTTATTTGGAGCTTTGGTTACTCTTTTTGGTGAACTAATTTTTACACCTGTTAATGAACAAGATATTGTCAATTTCATCTTTGTTGGTACAGGAAATAACCAGATTTATTTCATGATGCATTTTCTCAGTTTACTTGTAAGCTTAGCAATTATCTTGTGGGATAACTGTTTTTCTTTAATCTCATTTTTCTATATCCATGTGTTTGCTCTAATTTACTTTAGTTATGTAGCTTTGATGGTGTCTGTGTTTAAGGACCAGATCACAGGTAATACAACTGGTATCTTAGCAAGTGATTGA", "aa": "MQTLNYLVIILIIAGVISVLAFTPLVRKLKIRFYLIQALAIVLFVYVFFGRQIIYLFPDVYGQNSQSSQTLNSLRLSRIFLLDLCPFFAVIAPVFVFLKQKKISGVLAVFGLFGALVTLFGELIFTPVNEQDIVNFIFVGTGNNQIYFMMHFLSLLVSLAIILWDNCFSLISFFYIHVFALIYFSYVALMVSVFKDQITGNTTGILASD", "aa_hexdigest": "9dc286f51cf163c314bd787bf31237e9", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 160058, "stop": 160900, "observations": { "mutation": [ 160273 ], "stop": [ 160273 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47381", "query_cov": 1.0, "subject_cov": 0.7464285714285714, "identity": 0.981, "score": 377.0, "evalue": 1.72e-130, "gene_start": 1, "gene_end": 209, "reference_start": 1, "reference_end": 209, "reference_sequence": "MQTLNYLVIILIIAGVISVLAFTPLVRKLKIRFYLIQVLAIVLFVYVFFGRQIIYLFPDVYGQNSQSSQNLDSLRLSRIFLLDLCPFFAVIAPVFVFLKQKKISGVLAVFGLFGALVTLFGELIFTPVNEQDIVNFIFVGTGNNQIYFMMHFLSLLVSLAIILWDNCFSLISFFYIHVFALIYFSYVALMVSVFKGQITGNTTGILASDWTNGEYKNVATFLNLSNSDPQLVFIVGFSLSYVAILLMTLFANIPTFMEMKKDKIFIKKENLIRKDLELLA" }, "paralog": false, "identity": 0.9821428571428571, "score": 529.0, "evalue": 5.51e-193, "description": "Internal stop codon at 160273. Nonsense mutation around 160273.", "psc": { "uniref90_id": "UniRef90_P47381", "product": "Uncharacterized protein MG135", "uniref50_id": "UniRef50_P47381", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47381", "UniRef:UniRef50_P47381", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47381", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47381" ], "product": "Uncharacterized protein MG135" } }, "id": "JPCNHPFPBM_327", "locus": "GCA000292505_00288" }, { "type": "cds", "sequence": "contig_1", "start": 160949, "stop": 161377, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01336.31" ], "nt": "ATGAGTGATCGTTTAAATGATCAAGCCCAACATCGCTTGCAGAAACTTTTAAGGTTAAAACAAACTAATAATGACCCTTATTTAGTAACAAAAACTAGTCTAACCCATTCTTCAAAAAGCTTTCAAGTTGAATTTGAAAAATGTGCAGAAGAAGAGTTGAAGAAAAAAACAACTGTCTCACTAGCTGGAAGGATCATTGCTATTAGACAAACCTTTTTAATTATTCAAGATTTTGATGGTCAAGTCCAACTTTACATCAATAAAAAAATCCATCCTAAGTTATTTGATTACTTTAATGAATTTGTTGATATTGGTGATCAAATTGTTGTTAGTGGTAAGCCAATGTTAACTAAAACAAAGGTATTAACTTTAGCTGTTGAAGAGATGAAAATCATTGCTAAGTGTTTATTGGTTCCACCTGAAAAGTGA", "aa": "MSDRLNDQAQHRLQKLLRLKQTNNDPYLVTKTSLTHSSKSFQVEFEKCAEEELKKKTTVSLAGRIIAIRQTFLIIQDFDGQVQLYINKKIHPKLFDYFNEFVDIGDQIVVSGKPMLTKTKVLTLAVEEMKIIAKCLLVPPEK", "aa_hexdigest": "aa2449725f59e95f0ce925ffb6d482a0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01336.31", "name": "tRNA_anti-codon", "length": 79, "aa_cov": 0.5211267605633803, "hmm_cov": 0.9493670886075949, "evalue": 2.1675303051188915e-13, "score": 45.133270263671875, "start": 59, "stop": 132 } ], "seq_stats": { "molecular_weight": 16363.982499999995, "isoelectric_point": 9.205779075622559 }, "id": "JPCNHPFPBM_328", "locus": "GCA000292505_00289" }, { "type": "cds", "sequence": "contig_1", "start": 161444, "stop": 161842, "strand": "+", "frame": 2, "gene": null, "product": "Lysine--tRNA ligase, heat inducible", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X2LQM9" ], "nt": "ATGCGTGATGTTTTTCTGAAACGCACTAAGATTATTAAATCAATCCGTAGCTTTCTTGATCAAAATGGTTTTATTGAGGTTGAAACCCCCACTTTACAAGCTGTTTTAGGAGGAGCTAATGCTAAACCCTTTAAAACCCATTACAATGCTTTAAAAGCGGATTTTTATCTCAGAATTGCTAATGAAATAGCATTAAAAAAACTCATTATTGGTGGATTTAATAAGGTTTATGAAATGGGTAAAATGTTCCGCAATGAAGGGGTTGATACTACCCACAATCCTGAGTTTACCAGTATTGAAATATATCAAGCTTATGCAGATTTTGAAGTCATGCTTGTGCTTGTTGAAAAGATGATTCAATCACTTTGTGAAAGCTTAAACCAATTTAGCTTTAACTGA", "aa": "MRDVFLKRTKIIKSIRSFLDQNGFIEVETPTLQAVLGGANAKPFKTHYNALKADFYLRIANEIALKKLIIGGFNKVYEMGKMFRNEGVDTTHNPEFTSIEIYQAYADFEVMLVLVEKMIQSLCESLNQFSFN", "aa_hexdigest": "1829c73eaefdb21b6434c19b0d7d8a0c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2X2LQM9", "query_cov": 0.9242424242424242, "subject_cov": 0.8652482269503546, "identity": 0.5489999999999999, "score": 149.0, "evalue": 4.7e-44, "valid": false, "product": "Lysine--tRNA ligase, heat inducible", "uniref50_id": "UniRef50_A0A2X2LQM9" }, "pscc": { "uniref50_id": "UniRef50_A0A2X2LQM9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X2LQM9" ], "product": "Lysine--tRNA ligase, heat inducible" }, "genes": [], "id": "JPCNHPFPBM_329", "locus": "GCA000292505_00290" }, { "type": "cds", "sequence": "contig_1", "start": 161888, "stop": 162421, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGGTTGAACTTATTAAGAAAGTTACAGGGATCGATTTTAATTCAGTAAAAGATGATCAATCTGCCATTTTATTAGCAGAAAAACACCATGTTAAACTAGCAAAACACCAACAAAATAAGCAACACATCATTAATTTGTTTTTTGAACAATTTTGTGAACAAACATTAATTGAACCTACCTTTGTAACCCATTATCCAAAAGCAGTTTCTCCTCTAGCAAAACAAGATCCTTCAAATCCTGAATTCACCCAACGATTTGAACTTTTTATTAATGGTAAAGAGATTGCTAATGCTTACAGCGAGCTAAACGATCCTTTAGAACAAAGAAAAAGGTTTGAACAACAACTTGAAGAAAAACAGCTTGGTAATGATGAGACAAGTGAACTTGATGAATCGTTTTTAGAAGCATTAAGTTTTGGGATGGTAAACACTGCTGGGCTTGGGATAGGTATTGATCGTTTGGTAATGTTGTTATGTGAATGTAATTCTATCCGTGATGTTGTTTTCTTCCCCCAGTTACGTGAACATAAATAG", "aa": "MVELIKKVTGIDFNSVKDDQSAILLAEKHHVKLAKHQQNKQHIINLFFEQFCEQTLIEPTFVTHYPKAVSPLAKQDPSNPEFTQRFELFINGKEIANAYSELNDPLEQRKRFEQQLEEKQLGNDETSELDESFLEALSFGMVNTAGLGIGIDRLVMLLCECNSIRDVVFFPQLREHK", "aa_hexdigest": "e535823dc2549131097509eb98553db5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0031D0E1C7", "query_cov": 0.9830508474576272, "subject_cov": 0.8093023255813954, "identity": 0.546, "score": 182.0, "evalue": 2.26e-55, "valid": false, "product": "amino acid--tRNA ligase-related protein", "ec_ids": [ "6.1.1.6" ], "uniref50_id": "UniRef50_A0A349PV97", "kegg_orthology_id": "K04567" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 127, "aa_cov": 0.6892655367231638, "hmm_cov": 0.968503937007874, "evalue": 1.4665041217694452e-37, "score": 124.41230773925781, "start": 52, "stop": 173 } ], "seq_stats": { "molecular_weight": 20391.9711, "isoelectric_point": 5.15656986236572 }, "id": "JPCNHPFPBM_330", "locus": "GCA000292505_00291" }, { "type": "cds", "sequence": "contig_1", "start": 162384, "stop": 163223, "strand": "+", "frame": 3, "gene": "glf", "product": "UDP-galactopyranose mutase", "db_xrefs": [ "COG:COG0562", "COG:M", "EC:5.4.99.9", "GO:0005829", "GO:0008767", "GO:0009273", "GO:0050660", "KEGG:K01854", "SO:0001217", "UniRef:UniRef50_P75499", "UniRef:UniRef90_Q49398" ], "nt": "ATGTTGTTTTCTTCCCCCAGTTACGTGAACATAAATAGTTTTGATATTTTAATTGTTGGTGCTGGTATTAGTGGAATAGTACTAGCTAACATCTTAGCTAACCACAATAAAAGGGTTTTAATTGTTGAAAAAAGAGATCATATTGGTGGTAACTGTTATGATAAAGTTGATAGTAAAACTCAACTCTTGTTTCACCAGTATGGACCCCATATTTTCCATACTAACAACCAAACTGTTATTAACTTTATCTCACCCTTCTTTGAACTAAATAACTACCACCATCGGGTTGGTTTAAAATTGAAAAATAACCTAGATTTAACCTTACCCTTTGATTTTCAACAGATCTATAAACTAATGGGAAAAGATGGTAGAAAACTCGTTAGTTTTTTTAAAGAAAATTTCAGTTTAAATAGTCATCTATCATTAGCAGAATTACAACTAATTGATAATCCTTTAGCACAAAAACTCTATCAGTTTTTAATTAGTAATGTTTATAAACCATACAGTGTCAAAATGTGGGGTTTACCATTTGCAATGATTAATGAGAATGTTATTAACAGGGTCAAGATAGTTTTAAGTGAACAAAGCAGTTATTTTCCTGATGCAATTATCCAGGGATTACCTAAATCAGGTTATACAAACAGTTTTCTTAAGATGTTAGCCAATCCCTTAATTGATGTGCAGTTAAACTGCAAAGATAACCTTTTAGTTTATCAAGATGAAAAACTGTTTTTTAACAATACTTTAATAGAAAAACCAGTTGTTTACTGTGGTTTAATTGACAAGTTATTTAACTTTTGCTTTGGTCATTTGCAATACCGTTCCCTTGCCTTTAGTTGA", "aa": "MLFSSPSYVNINSFDILIVGAGISGIVLANILANHNKRVLIVEKRDHIGGNCYDKVDSKTQLLFHQYGPHIFHTNNQTVINFISPFFELNNYHHRVGLKLKNNLDLTLPFDFQQIYKLMGKDGRKLVSFFKENFSLNSHLSLAELQLIDNPLAQKLYQFLISNVYKPYSVKMWGLPFAMINENVINRVKIVLSEQSSYFPDAIIQGLPKSGYTNSFLKMLANPLIDVQLNCKDNLLVYQDEKLFFNNTLIEKPVVYCGLIDKLFNFCFGHLQYRSLAFS", "aa_hexdigest": "61f97aacc7777409a662d1c6cccc3038", "start_type": "ATG", "rbs_motif": null, "genes": [ "glf" ], "truncated": "3-prime", "pseudogene": { "start": 162363, "stop": 163526, "observations": { "mutation": [ 163221, 163455 ], "stop": [ 163221, 163455 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49398", "query_cov": 1.0, "subject_cov": 0.6905940594059405, "identity": 0.9890000000000001, "score": 557.0, "evalue": 1.33e-198, "gene_start": 1, "gene_end": 279, "reference_start": 8, "reference_end": 286, "reference_sequence": "MNVILSVMLFSSPSCVNINSFDILIVGAGISGIVLANILANHNKRVLIVEKRDHIGGNCYDKVDSKTQLLFHQYGPHIFHTNNQTVINFISPFFELNNYHHRVGLKLKNNLDLTLPFDFQQIYKLMGKDGRKLVSFFKENFSLNTHLSLAELQLIDNPLAQKLYQFLISNVYKPYSVKMWGLPFAMINENVINRVKIVLSEQSSYFPDAIIQGLPKSGYTNSFLKMLANPLIDVQLNCKDNLLVYQDEKLFFNNNLIEKPVVYCGLIDKLFNFCFGHLQYRSLAFSWKRFNQKKYQTYPVVNMPLAKSITRSVEYKQLTNQGSFKPQTIVSFETPGSYAINDPRFNEPYYPINNTLNDTLFKKYWKKASKLKNLHLLGRLATYQYIDMDKAILLSIKKAQQLLS" }, "paralog": false, "identity": 0.9844961240310077, "score": 769.0, "evalue": 1.16e-284, "description": "Internal stop codon at 163221, 163455. Nonsense mutation around 163221, 163455.", "psc": { "uniref90_id": "UniRef90_Q49398", "gene": "glf", "product": "UDP-galactopyranose mutase", "ec_ids": [ "5.4.99.9" ], "uniref50_id": "UniRef50_P75499", "cog_id": "COG0562", "cog_category": "M", "kegg_orthology_id": "K01854", "go_ids": [ "GO:0005829", "GO:0008767", "GO:0009273", "GO:0050660" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49398", "EC:5.4.99.9", "UniRef:UniRef50_P75499", "COG:COG0562", "COG:M", "KEGG:K01854", "GO:0005829", "GO:0008767", "GO:0009273", "GO:0050660" ] }, "pscc": { "uniref50_id": "UniRef50_P75499", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75499" ], "product": "UDP-galactopyranose mutase" } }, "id": "JPCNHPFPBM_331", "locus": "GCA000292505_00292" }, { "type": "cds", "sequence": "contig_1", "start": 163269, "stop": 163457, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTTTAGCTAAATCAATCACAAGGAGTGTGGAATACAAACAACTAACAAACCAAGGTTCTTTCAAACCGCAAACCATCGTTAGTTTTGAAACCCCTGGCAGCTATGCAATTAACGATCCTAGGTTTAATGAACCTTATTACCCAATTAACAATACACTAAATGATACTCTTTTTAAAAAGTACTGA", "aa": "MPLAKSITRSVEYKQLTNQGSFKPQTIVSFETPGSYAINDPRFNEPYYPINNTLNDTLFKKY", "aa_hexdigest": "6b8a697082f073d04bad490dc692dfc9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7178.007700000001, "isoelectric_point": 9.04899196624756 }, "id": "JPCNHPFPBM_332", "locus": "GCA000292505_00293" }, { "type": "cds", "sequence": "contig_1", "start": 163577, "stop": 165373, "strand": "+", "frame": 2, "gene": "lepA", "product": "translation elongation factor 4", "db_xrefs": [ "COG:COG0481", "COG:J", "EC:3.6.5.n1", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005886", "GO:0043022", "GO:0045727", "GO:1990606", "RefSeq:WP_014894582.1", "SO:0001217", "UniParc:UPI00027B3659", "UniRef:UniRef100_UPI00027B3659", "UniRef:UniRef50_B1X3K4", "UniRef:UniRef90_P47384" ], "nt": "ATGGAACAAAAAAACATTAGAAATTTTTCTATTATTGCCCATATTGATCATGGTAAATCTACCTTATCAGACCGCTTGTTAGAACATAGTTTAGGCTTTGAAAAAAGACTATTACAAGCGCAAATGCTTGATACTATGGAGATTGAAAGAGAAAGGGGTATTACCATTAAATTAAATGCTGTTGAGTTGAAAATTAATGTTGATAACAATAACTATCTTTTTCATTTAATTGACACCCCTGGGCATGTTGATTTTACTTATGAAGTGTCTCGTTCTTTAGCAGCTTGTGAGGGAGTTTTATTGTTAGTAGATGCAACCCAAGGAATTCAAGCACAAACGATTTCCAATGCCTATCTTGCGTTGGAAAATAACCTGGAAATTATCCCAGTCATTAACAAGATAGATATGGATAATGCTGATATTGAAACAACAAAAGATTCACTCCATAACCTATTAGGAGTTGAAAAAAACAGTATCTGTTTAGTATCTGCAAAAGCTAACTTAGGGATTGATCAGTTAATTCAAACAATTATAGCTAAGATCCCTCCACCAAAAGGAGAAATTAATAAACCTTTAAAAGCATTACTCTTTGATAGTTACTATGACCCTTACAAGGGGGTTGTTTGTTTTATTAGAGTATTTGATGGTTGTTTGAAGGTTAATGATAAGGTTCGTTTTATTAAAAGTAATTCTGTTTACCAAATTGTGGAACTAGGGGTTAAAACCCCATTTTTTGAAAAAAGAGATCAATTGCAAGCAGGGGATGTTGGTTGGTTTTCAGCAGGGATAAAAAAACTCCGTGATGTTGGGGTTGGTGATACTATTGTGAGTTTTGACGATCAATTTACAAAACTCTTAGCAGGTTATAAAAAGATCTTACCCATGATCTATTGTGGTTTATATCCAGTTGATAACAGTGATTATCAAAACCTCAAGTTAGCGATGGAAAAGATCATAATCAGTGATGCAGCATTGGAATATGAATATGAAACATCCCAAGCGTTAGGGTTTGGGGTTAGGTGTGGTTTTCTAGGTCTTTTACATATGGATGTTATTAAAGAAAGATTGGAAAGAGAATACAACCTAAAACTCATCTCAGCTCCCCCTTCAGTTGTATATAAGGTATTGTTAACCAATGGTAAAGAGATTAGTATTGATAATCCCTCTTTGTTACCAGAACGCTCCAAGATTAAAGCAATCAGTGAACCATTTGTAAAAGTTTTTATTGATTCACCTGATCAATATTTGGGCAGTGTTATTGATTTGTGCCAAAACTTCAGGGGTCAATATGAAAGTTTAAATGAGATTGATATCAACAGAAAAAGGATCTGTTATCTGATGCCTTTAGGGGAAATTATCTACAGTTTTTTTGATAAGTTAAAGTCGATTAGTAAGGGTTATGCATCGTTAAACTATGAGTTTTATAACTACCAACATAGTCAACTGGAAAAAGTTGAGATCATGTTAAACAAACAAAAGATTGATGCATTATCTTTTATCAGTCATAAAGACTTTGCTTTTAAGCGGGCAAAAAAGTTTTGCACTAAGCTCAAAGAATTGATTCCAAAGCATCTGTTTGAGATCCCTATCCAAGCAACAATAGGGAGTAAAGTAATAGCAAGAGAGACAATCAAAGCAGTTAGAAAGGATGTAATAGCTAAACTTTATGGAGGGGATGTTAGTAGAAAAAAGAAGTTATTAGAGAAGCAAAAAGAGGGTAAAAAACGCTTGAAAGCAGTTGGGAGTGTTCAATTACCTCAAGAGCTATTTAGTCATTTGCTGAAAGATGAAGATTAA", "aa": "MEQKNIRNFSIIAHIDHGKSTLSDRLLEHSLGFEKRLLQAQMLDTMEIERERGITIKLNAVELKINVDNNNYLFHLIDTPGHVDFTYEVSRSLAACEGVLLLVDATQGIQAQTISNAYLALENNLEIIPVINKIDMDNADIETTKDSLHNLLGVEKNSICLVSAKANLGIDQLIQTIIAKIPPPKGEINKPLKALLFDSYYDPYKGVVCFIRVFDGCLKVNDKVRFIKSNSVYQIVELGVKTPFFEKRDQLQAGDVGWFSAGIKKLRDVGVGDTIVSFDDQFTKLLAGYKKILPMIYCGLYPVDNSDYQNLKLAMEKIIISDAALEYEYETSQALGFGVRCGFLGLLHMDVIKERLEREYNLKLISAPPSVVYKVLLTNGKEISIDNPSLLPERSKIKAISEPFVKVFIDSPDQYLGSVIDLCQNFRGQYESLNEIDINRKRICYLMPLGEIIYSFFDKLKSISKGYASLNYEFYNYQHSQLEKVEIMLNKQKIDALSFISHKDFAFKRAKKFCTKLKELIPKHLFEIPIQATIGSKVIARETIKAVRKDVIAKLYGGDVSRKKKLLEKQKEGKKRLKAVGSVQLPQELFSHLLKDED", "aa_hexdigest": "376154b47f93dd5b1ca194cd8b78b888", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3659", "ncbi_nrp_id": "WP_014894582.1", "uniref100_id": "UniRef100_UPI00027B3659", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3659", "RefSeq:WP_014894582.1", "UniRef:UniRef100_UPI00027B3659" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3659", "uniref90_id": "UniRef90_P47384" }, "psc": { "uniref90_id": "UniRef90_P47384", "gene": "lepA", "product": "translation elongation factor 4", "ec_ids": [ "3.6.5.n1" ], "uniref50_id": "UniRef50_B1X3K4", "cog_id": "COG0481", "cog_category": "J", "go_ids": [ "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005886", "GO:0043022", "GO:0045727", "GO:1990606" ] }, "genes": [ "lepA" ], "id": "JPCNHPFPBM_333", "locus": "GCA000292505_00294" }, { "type": "cds", "sequence": "contig_1", "start": 165457, "stop": 167115, "strand": "+", "frame": 1, "gene": "rnj", "product": "Ribonuclease J", "db_xrefs": [ "COG:COG0595", "COG:J", "EC:3.1.-.-", "GO:0003723", "GO:0004521", "GO:0004534", "GO:0005737", "GO:0006364", "GO:0008270", "SO:0001217", "UniRef:UniRef50_P75497", "UniRef:UniRef90_P47385" ], "nt": "ATGATAAAGGATTTTAATCCTGGTGATTTTATTGGTAAAAAACCAACTAAAATCTATGCTTTTGGTGGTATCCAAGAAGTTGGTAAAAACATGTATGGGATTGAATATGATGATGAAATCATCATTATTGACTGTGGCATTAAATTTGCTAGTGATGATCTACTTGGTATCAATGGGATTATCCCTAGTTTTGAACACTTAATTGAAAACCAAAGTAAGGTTAAAGCATTGTTTATTACCCATGGTCATGAAGACCATATTGGGGGTGTACCATACCTTTTAAAGCAGGTTGATATTCCTGTTATCTACGCACCAAGGATCGCAGCATCATTAATCTTGAAAAAGGTTAATGAGCACAAGGATGCTAAGCTCAATAAGATAGTTACTTTTGATGATTTTAGTGAGTTTCAAACCAAACACTTCAAAATTGATTTTTACCGGGTAAACCACTCGATTCCCGATGCTTTTGGAATCTGTGTGCAAACCCCTAATGGCAACATTGTTCAAAGTGGTGACTACCGGTTTGATTTTGCTGCTGGGAGTGAGATGTTAGATGTTCATAAAGTAGTGAAAATTGCTGAGCGTAATGTCCATGTTTTTATGAGTGAATCTACTAATGCTGAAGTACCAGGTTTTTCCCAAAGTGAAAAGTTAATTTACAGAAACATCCAAAAGATCTTAAAAGAAGCAAGAGGTAGGGTTATTTTAACTACCTTTGCATCCAACATCACACGGATTAATGAAATTATTGAGATTGCTTTAAACAACAAGCGTAAGATCTGTTTATTGGGCAAATCAATGGATGTTAATGTCAATATTTCGCGCAAAATTGGATTGATGGCAATTGATAGTAATGATATTGTGGAAGTCCGTGATATCAAAAACTATCCTGATCATAATATCTTAATCTTGTGTACTGGTTCACAAGGCGAGGAGGCTGCTGCTTTAAACACGATGGCACGTGGTAAGCATAATTGGGTAAGCTTAAAATCAACTGACACCATCATTATGTCTTCAAATCCAATTCCAGGTAATTATGCTGCAGTTGAAAACTTGCTTAATGAACTATCTAAGTTTGGTGTTGCTATTTATGAAAATTCATCCCAACTAAAACTACATGCCTCAGGTCATGCCACCCAACAAGAGTTACAGTTGATGCTAAATTTAATGTTTCCTAAATACTTAATTCCTATCCATGGTGAATTTAAGATGATGCGAACCATAAAAAACATTGCTAATGAATGTGGTATTAAAAGCGAGGATGTGGCACTTTTAAGTAATGGCCAAGTAATGTATTTAATTGATGAAGAACTTTATTATTCCAATGAAATTATTAATGCTGATCCTATTTATATAGAGAGTCATAACTCTTCTCCTGATCTTGCAAGAATAATTAAGCAAAGACAAATCCTTAGTCGTGATGGGATGTTTGCTGTTATTGTTGTTTTTGATAAAAATAACAACATCATTGGGATTCCAACCTTAATAACAAGAGGTTGTTTCTTTGCACTTGATTCCAATCCTTTAATGACAAAGATAGCCCATTCTGTTAAAAGAACTTTAGAAAGTGTTATCCAAAGTAAGAAGTTTAATAGTCATGAACAACTAACAAAGGAATTGAAACGAGTTTGTAAGGAAACTGTTTCTTACTTTATCTGA", "aa": "MIKDFNPGDFIGKKPTKIYAFGGIQEVGKNMYGIEYDDEIIIIDCGIKFASDDLLGINGIIPSFEHLIENQSKVKALFITHGHEDHIGGVPYLLKQVDIPVIYAPRIAASLILKKVNEHKDAKLNKIVTFDDFSEFQTKHFKIDFYRVNHSIPDAFGICVQTPNGNIVQSGDYRFDFAAGSEMLDVHKVVKIAERNVHVFMSESTNAEVPGFSQSEKLIYRNIQKILKEARGRVILTTFASNITRINEIIEIALNNKRKICLLGKSMDVNVNISRKIGLMAIDSNDIVEVRDIKNYPDHNILILCTGSQGEEAAALNTMARGKHNWVSLKSTDTIIMSSNPIPGNYAAVENLLNELSKFGVAIYENSSQLKLHASGHATQQELQLMLNLMFPKYLIPIHGEFKMMRTIKNIANECGIKSEDVALLSNGQVMYLIDEELYYSNEIINADPIYIESHNSSPDLARIIKQRQILSRDGMFAVIVVFDKNNNIIGIPTLITRGCFFALDSNPLMTKIAHSVKRTLESVIQSKKFNSHEQLTKELKRVCKETVSYFI", "aa_hexdigest": "8dc5fb6f8f91f4cfa229d8a6f860f283", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47385", "query_cov": 1.0, "subject_cov": 0.9701230228471002, "identity": 0.998, "score": 1089.0, "evalue": 0.0, "valid": true, "gene": "rnj", "product": "Ribonuclease J", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75497", "cog_id": "COG0595", "cog_category": "J", "go_ids": [ "GO:0005737", "GO:0004534", "GO:0003723", "GO:0004521", "GO:0008270", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_P75497", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75497" ], "product": "Ribonuclease J" }, "genes": [ "rnj" ], "id": "JPCNHPFPBM_334", "locus": "GCA000292505_00295" }, { "type": "cds", "sequence": "contig_1", "start": 167185, "stop": 167538, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTACTAGTGCCTAAGGTGGTAGTGGAGTATCACAAGCTCAGTAAGGATGTAGTCAAAGAGAGTTTGGAAGTGGAAGCAACTTCCACCTTCAACCCCACCCAAAGGTTGCAAAAAGATAGTCCAATGAAGGATTCAGCAAGGGAAAGTCAAGAGAAACTCCAAGAGACCATGTCATCAATGAGTGGTATGGCTACATCCACTCGAGCCAAGGCCCTTAAGATAGAGGTGGAAAGGGGGAGTAATGTCAATCAAGGCGAACTACAATCCAACGACTTTGCTAAAAAGCCGTTTAAAGATGAGAGCAATAAGAAGTTGGATGCGAGTGGGGAGTTTGCAGGAGGCAAGGCCTGA", "aa": "MLLVPKVVVEYHKLSKDVVKESLEVEATSTFNPTQRLQKDSPMKDSARESQEKLQETMSSMSGMATSTRAKALKIEVERGSNVNQGELQSNDFAKKPFKDESNKKLDASGEFAGGKA", "aa_hexdigest": "533a4e2beaead8c08bbdbeab1e054cf0", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030D4FD3B", "query_cov": 0.9572649572649573, "subject_cov": 0.875, "identity": 0.759, "score": 132.0, "evalue": 1.74e-37, "valid": false, "product": "adhesin", "uniref50_id": "UniRef50_A0A7M3VDG5" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12909.441199999983, "isoelectric_point": 7.904355812072754 }, "id": "JPCNHPFPBM_335", "locus": "GCA000292505_00296" }, { "type": "cds", "sequence": "contig_1", "start": 167552, "stop": 167719, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCATTGATTTAACCCCAACATTGATGTTAGAAAAAGTGGGGAGGGGGGTAGAAGAAGCAGAAGTTGAAGGGGAGGGGGAGTTGGAGAAGGGTTTGTTGTTGCCGATTCAGGGGAGAAAGTCTAAGTCGCCCCCATCCCCCTATTTTTTTTTATTTGTTCGGTAG", "aa": "MIIDLTPTLMLEKVGRGVEEAEVEGEGELEKGLLLPIQGRKSKSPPSPYFFLFVR", "aa_hexdigest": "be05471996ce79390e9aca63573e34e6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6146.1358, "isoelectric_point": 4.9672960281372065 }, "id": "JPCNHPFPBM_336", "locus": "GCA000292505_00297" }, { "type": "cds", "sequence": "contig_1", "start": 167875, "stop": 168168, "strand": "+", "frame": 1, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FCDE6DE", "UniRef:UniRef90_UPI002FCDE6DE" ], "nt": "GTGGCTCCTACCAAAAAAACAACCACGTATGATGCTTTACCCAACTCCATCAGTCCCACCAGTGACTGGATCAATGCATTGACTTTCACCAATAAGAATAATCCACAACGAAATCAGTTGTTGTTAAGAGCACTGTTAGGAACGATCCCGGTATTGATCAATAAGAGCGGAGGAAGTGGGAATGAGTTTAACCATACGAATGATCAGAAGTGGGATAAAACGAATGAAAAAGATGGGAATTTACCTGGGTTTGGGGAGGTGAATGGTGGTTTTTACCCAGTTTTACTTATTTAA", "aa": "MAPTKKTTTYDALPNSISPTSDWINALTFTNKNNPQRNQLLLRALLGTIPVLINKSGGSGNEFNHTNDQKWDKTNEKDGNLPGFGEVNGGFYPVLLI", "aa_hexdigest": "0bb01dfe4c38ccffe5213b7a9e743a3a", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 167712, "stop": 168175, "observations": { "insertion": [ 167895 ], "deletion": [ 168145, 168157 ], "mutation": [ 167781 ], "stop": [ 167781 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FCDE6DE", "query_cov": 0.9484536082474226, "subject_cov": 0.5542168674698795, "identity": 0.924, "score": 176.0, "evalue": 8.07e-55, "gene_start": 6, "gene_end": 97, "reference_start": 72, "reference_end": 163, "reference_sequence": "MITQHLNKENTRWVFTSGSTPDIWTGAGYRKGNDNNNGIPLTSVKPSSNNNQQFNPNSDDNKVTSGGGTQTKKTTYDALPNSISPTSDWSNALTFTNKNNPQRNQLLLRALLGTIPVLINKSGGSGNEFNHTNDQKWDKTNEKDGNLPGFGEVNGGFLPSFYLFNS" }, "paralog": false, "identity": 0.8012048192771084, "score": 255.0, "evalue": 1.88e-88, "description": "Internal stop codon at 167781. Frameshift due to insertion around 167895. Frameshift due to deletion around 168145, 168157. Nonsense mutation around 167781.", "psc": { "uniref90_id": "UniRef90_UPI002FCDE6DE", "product": "adhesin", "uniref50_id": "UniRef50_UPI002FCDE6DE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FCDE6DE", "UniRef:UniRef50_UPI002FCDE6DE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FCDE6DE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FCDE6DE" ], "product": "adhesin" } }, "id": "JPCNHPFPBM_337", "locus": "GCA000292505_00298" }, { "type": "cds", "sequence": "contig_1", "start": 168589, "stop": 168765, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13", "UniRef:UniRef90_UPI0030D43D23" ], "nt": "GTGGTGAAAAAGTATCAGTTAGATGTGCCAAGTGTTTCAACAACCATGAACCAAGTTTTGCAAGAATTGATCCTTGAACAACCTTTGACTAAATATACCTTAAATTCGAGTTTGGCCAAACAGAAGGGTAAGACACAAGCAGTGGTGCATCTTGGTTCAAATATGGCAAATCAGTGA", "aa": "MVKKYQLDVPSVSTTMNQVLQELILEQPLTKYTLNSSLAKQKGKTQAVVHLGSNMANQ", "aa_hexdigest": "c2c0c9166a836c4b4cb494f36625568e", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 168469, "stop": 168830, "observations": { "deletion": [ 168787 ], "mutation": [ 168583, 168763 ], "stop": [ 168583, 168763, 168787 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0030D43D23", "query_cov": 0.9827586206896551, "subject_cov": 0.47107438016528924, "identity": 0.825, "score": 90.1, "evalue": 4.9e-22, "gene_start": 2, "gene_end": 58, "reference_start": 43, "reference_end": 99, "reference_sequence": "MKLTYSSSGSNTNTISFENNANKPSYVVEFTNSTNVGIKWRVVKKYQLDVPNVSSDMNQVLQELILEQPLTKYTLNSSLAKQKGKSKIEVHLGSGRATQWTSRTQLHWPKQQSQPKCINRI" }, "paralog": false, "identity": 0.71900826446281, "score": 155.0, "evalue": 1.32e-50, "description": "Internal stop codon at 168583, 168763, 168787. Frameshift due to deletion around 168787. Nonsense mutation around 168583, 168763.", "psc": { "uniref90_id": "UniRef90_UPI0030D43D23", "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0030D43D23", "UniRef:UniRef50_UPI00027B3D13" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6446.430100000003, "isoelectric_point": 9.523801612854005 }, "id": "JPCNHPFPBM_338", "locus": "GCA000292505_00299" }, { "type": "cds", "sequence": "contig_1", "start": 168893, "stop": 169237, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGACCAAGCAGGGCAAAGGGAAGGATAGTAGTGGGTGGAATAGTGAAGAAAACAATGGCAGCAGGGGATGCGCCCAGTGTTACTGCAGGGGGAAGTGCTTCTGGATCATTTAATAAATACCTCAACACCAAACAAGCGTTAGAAAGCATCGGCATCTTGTTTGATGGGGATGGAATGAGGAATGTTATCACCCAACTCTATTATGCTTCTACCAGTAAGCTAGCAGTCACTAACAACCACATTGTCGTGATGGGTAACAGCTTTCTACCCAGCTTGTGGTACTGGGTGGTGGATCGGGGTGCAACAACTGATTCATCATCAAAAACCCACCTGGTTTGCTAA", "aa": "MGPSRAKGRIVVGGIVKKTMAAGDAPSVTAGGSASGSFNKYLNTKQALESIGILFDGDGMRNVITQLYYASTSKLAVTNNHIVVMGNSFLPSLWYWVVDRGATTDSSSKTHLVC", "aa_hexdigest": "88893904f8cb4f6cbe1fb3c4542b1603", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12014.607599999988, "isoelectric_point": 9.609931373596194 }, "id": "JPCNHPFPBM_339", "locus": "GCA000292505_00300" }, { "type": "cds", "sequence": "contig_1", "start": 169427, "stop": 169651, "strand": "+", "frame": 2, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030D266AC", "UniRef:UniRef90_UPI00027B3D0D" ], "nt": "GTGACCAACGACACGGGTTTAAATGGTCCAATCAATGGGAGTTTTTCAATCCAAGACACCTTTTCCTTCGTTGTTCCTTATTCAGATAGTCATAGTAATCAAACTTCATCAGGAACCATTAAAACGGCTTATCCGGTGAAAAGTGATCAAAAATCATCAGTAGCGATCAATTCTTTGATTAACGCTACGCCGTTGAATAGTTATGGGGATTTAAACATTAAATAA", "aa": "MTNDTGLNGPINGSFSIQDTFSFVVPYSDSHSNQTSSGTIKTAYPVKSDQKSSVAINSLINATPLNSYGDLNIK", "aa_hexdigest": "ad1fa4d49c9b5d14117a965dd872bec9", "start_type": "GTG", "rbs_motif": null, "genes": [ "mgpC" ], "truncated": "3-prime", "pseudogene": { "start": 169457, "stop": 169686, "observations": { "deletion": [ 169643 ], "stop": [ 169646 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00027B3D0D", "query_cov": 0.8108108108108109, "subject_cov": 0.6060606060606061, "identity": 0.883, "score": 102.0, "evalue": 6.99e-27, "gene_start": 13, "gene_end": 72, "reference_start": 26, "reference_end": 85, "reference_sequence": "MTSLLKLLSSKNIKQKLGKGGTAMQGSFSVQDTFSFVVPYSGNHSNQISSGTIKTAYPVKKDQKSTVKINSLINATPLNSYGDLNNHTKLIYPNYLLIY" }, "paralog": false, "identity": 0.8289473684210527, "score": 123.0, "evalue": 4.46e-38, "description": "Internal stop codon at 169646. Frameshift due to deletion around 169643.", "psc": { "uniref90_id": "UniRef90_UPI00027B3D0D", "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI0030D266AC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00027B3D0D", "UniRef:UniRef50_UPI0030D266AC" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0030D266AC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030D266AC" ], "product": "MgpC family cytadherence protein" } }, "id": "JPCNHPFPBM_340", "locus": "GCA000292505_00301" }, { "type": "rRNA", "sequence": "contig_1", "start": 169848, "stop": 171365, "strand": "+", "gene": "rrs", "product": "16S ribosomal RNA", "coverage": 0.9902152641878669, "score": 1441.0, "evalue": 2.1e-44, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00177", "KEGG:K01977", "SO:0001000" ], "nt": "TTTTCTGAGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCATGCCTAATACATGCAAGTCGATCGGAAGTAGCAATACTTTAGAGGCGAACGGGTGAGTAACACGTATCCAATCTACCTTATAATGGGGGATAACTAGTTGAAAAACTAGCTAATACCGCATAAGAACTTTAGTTCGCATGAATTAAAGTTGAAAGGACCTGCAAGGGTTCGTTATTTGATGAGGGTGCGCCATATCAGCTAGTTGGTAGGGTAATGGCCTACCAAGGCAATGACGTGTAGCTATGCTGAGAAGTAGAATAGCCACAATGGGACTGAGACACGGCCCATACTCCTACGGGAGGCAGCAGTAGGGAATTTTTCACAATGAGCGAAAGCTTGATGGAGCAATGCCGCGTGAACGATGAAGGTCTTTTTGATTGTAAAGTTCTTTTATTTGGGAAGAATGACTCTAGCAGGCAATGGCTGGAGTTTGACTGTACCACTTTGAATAAGTGACGACTAACTATGTGCCAGCAGTCGCGGTAATACATAGGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCAAGCGCAGGCGGATTGAAAAGTCTGGTGTTAAAGGCAGCTGCTTAACAGTTGTATGCATTGGAAACTATCAGTCTAGAGTGTGGTAGGGAGTTTTGGAATTTCATGTGGAGCGGTGAAATGCGTAGATATATGAAGGAACACCAGTGGCGAAGGCGAAAACTTAGGCCATTACTGACGCTTAGGCTTGAAAGTGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACACCGTAAACGATAGATACTAGCTGTCGGAGCGATCCCTTCGGTAGTGAAGTTAACACATTAAGTATCTCGCCTGGGTAGTACATTCGCAAGAATGAAACTCAAACGGAATTGACGGGGACCCGCACAAGTGGTGGAGCATGTTGCTTAATTCGACGGTACACGAAAAACCTTACCTAGACTTGACATCCTTGGCAAAGTTATGGAAACATAATGGAGGTTAACCGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCGTTAGTTACATTGTTTAACGAGACTGCTAATGTAAATTGGAGGAAGGAAGGGATGACGTCAAATCATCATGCCCCTTATGTCTAGGGCTGCAAACGTGCTACAATGGCCAATACAAACAGTAGCCAACTTGTAAAAGTGAGCAAATCTGAAAAGTTGGTCTCAGTTCGGATTGAGGGCTGCAATTCGTCCTCATGAAGCTGGAATCACTAGTAATCGCGAATCAGCTATGTCGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACTATGAAAGCTGGTAATATTTAAAAACGTGTTGCTAACCTTTATTGGAAGTGCATGTCAAGGATAGCACCGGTGATTGGAGTTAAGTCGTAACAAGGTACCCCTACGAGAACGTGGGGGTGGATCACCTCCTTT", "id": "JPCNHPFPBM_38", "locus": "GCA000292505_00302" }, { "type": "rRNA", "sequence": "contig_1", "start": 171566, "stop": 174481, "strand": "+", "gene": "rrl", "product": "23S ribosomal RNA", "coverage": 0.9969230769230769, "score": 2679.0, "evalue": 6.1e-06, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF02541", "KEGG:K01980", "SO:0001001" ], "nt": "CAATAAGTTACTAAGGGCTTATGGTGGATGCCTTGGCACTAAAAGGCGATGAAGGACGTGTTAACCTGCGATAAGCTTCGGGGAGGTGGTAAAAACCTGAGATCCGGAGGTGTCCGAATGGAGCAATCTGGTAGCTTGCAAAAGTTACCATTAATTAATGAATTCATAGTTAATTAAAGCGATACGTGGTGAAGTGAAACATCTCAGTAACCACAGGAAAAGAAAACGAATGTGATTCCGTGTGTAGTGGCGAGCGAAAGCGGAACAGGCCAAACCTATCTGAGGATAGGGGTTGTAGGGCTTGCATTATGGAAGTTAAAAGGATAGAAGAAGCTGTTGGAAAGCAGCGCCAAAGAGGGTGATAGCCCCGTATTTGAAATCTTTTTAATACCTAGCAAGAAACCTGAGTAGCTCGAAAAACGTTATTTTGAGTGAATCTGCCCAGACCATTGGGTAAGCCTAAATACTAATTAGTGACCGATAGCGAAACAGTACCGTGAGGGAAAGGTGAAAAGAACCCAGAGATGGGAGTGAAATAGATTCTGAAACCATATGCCTACAACGTGTCAGAGCACATTAATGTGTGATGGCGTGCGTTTTGAAGTATGAGCCGGCGAGTTATGATAGCAAGCGTTAGTTAACCAGGAGATGGGGAGCTGTAGCGAAAGCGAGTTTTAAGAGAGCGTTTGTTTGTTATCATAGACCCGAAACGGGTTGAGCTAGTCATGAGCAGGTTGAAGGTTGAGTAACATTAACTGGAGGACCGAACCGACTCTCGTTGAAACGATAGCGGATGACTTGTGATTAGGGGTGAAATTCCAATCGAAATCCGTGATAGCTGGTTCTCGTCGAAATAGCTTTAAGGCTAGCGTAAGATCACAAATAAGTGGAGGTAAAGCTACTGAATGTATGATGGCGCCACCTAGGCGTACTGAATACAATTAAACTCTGAATGCCATTTATTTTATTCTTGCAGTCAGACAGTGGGGGATAAGCTTCATTGTCAAGAGGGGAAGAGCCCAGATCATTAAATAAGGTCCCCAAAATATACTAAGTGGAAAAGGATGTGAAAGTGCTAAAACAGCAAGGATGTTGGCTTAGAAGCAGCCATCGTTTAAAGAGTGCGTAACAGCTCACTTGTCGAGTGTTTTTGCGCCGAAGATGTAACGGGGCTAAGTATATTACCGAATTTATGGATAAGATATTTTTATCTTGTGGTAGACGAGCGTTGTATTGGAGTTGAAGTCAAAGCGTGAGCATTGGTGGATCCAATACAAGTGAGAATGCCGGCGTGAGTAACGCTTGGGAGTGAGAATCTCCCAAACCGATTGACTAAGGTTTCCTGGACCAGGGTCGTCCTTCCAGGGTTAGTCTGGACCTAAGCTGAGGCTGAAGAGCGTAGGCGATGGACAACAGGTTAATATTCCTGTACTTACAGTTAGACTGATGGAGTGACAAAGAAGGTTATCCACCCCCATTATTGGATTTGGGGATAAACTATAAGGTGGTACAATAGGCAAATCCGTTGTGCATAACACTGAGTGGTGATGTCGAGTGAACGAGTGATCAAGTAGCGAAGGTGGCAATTAATCATGCTTTCAAGAAAAGCTGCTAGGGCTAATTTAACTGTAACCAGTACCGAGAACGAACACACGTAGTCAAGGAGAGGATCCTAAGGTTAGCGAGTGAACTATAGCCAAGGAACTCTGCAAATTAACCCCGTAAGTTAGCGAGAAGGGGTGCTTATCTAAAAGTAAGCCGCAGTGAAGAACGAGGGGGGACTGTTTAACTAAAACACAACTCTATGCCAAACCGTAAGGTGATGTATATGGGGTGACACCTGCCCAGTGCTGGAAGGTTAAAGAAGGAGGTTAGCAATTTATTGCAAAGCTTTTAACTGAAGCCCCAGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTAGTCGGGTAAATTCCGTCCCGCTTGAATGGTGTAACCATCTCTTGACTGTCTCGGCTATAGACTCGGTGAAATCCAGGTACGGGTGAAGACACCCGTTAGGCGCAACGGGACGGAAAGACCCCGTGAAGCTTTACTGTAGCTTAATATTGATCAAAACACCACCATGTAGAGAATAGGTAGGAGCAATTGATGCAAGTTCGCAAGGATTTGTTGATGTGAAATGTGGAATACTACCCTTGGTTATGTTTTGTTCTAATTGGTAACTGTGATCCAGTTTCAAGACAGTGTTAGGTGGGCAGTTTGACTGGGGCGGTCGCCTCCTAAAAGGTAACGGAGGCGCACAAAGGTACCTTCAGTACGGTTGGAAATCGTATTTAGAGTGTAATGGTATAAGGGTGCTTGACTGTGAGACTTACAGGTCGAACAGGTGAGAAATCAGGTCATAGTGATCCGGTGGTTCAGTATGGAATGGCCATCGCTCAACGGATAAAAGCTACTCCGGGGATAACAGGCTGATACTGCCCAAGAGTTCATATCGACGGCAGTGTTTGGCACCTCGATGTCGACTCATCTCATCCTCGAGCTGAAGCAGGTTCGAAGGGTTCGGCTGTTCGCCGATTAAAGAGATACGTGAGTTGGGTTCAAACCGTCGTGAGACAGGTTGGTCCCTATCTATTGTGCCCACAGGAAGATTGAAGAGCTTTGCTTCTAGTACGAGAGGACCGGAGCGAGGACACCGCTTATGCTCCAGTTGTAGCGCCAGCTGCACCGCTGGGTAGTAACGTGTCTATTAGATAAACGCTGAAAGCATCTAAGTGTGAAACTATCTCAAAGATTAATCTTCCCATTTCTGTTAAAGGAAAGTAAGAGCCGTTATAGACCATGACGTTGATAGGTTACAGGTGTAAGCATAGTGATATGTTGAGCTGAGTAATACTAATTGCTCGAGGACTTATTGGT", "id": "JPCNHPFPBM_40", "locus": "GCA000292505_00303" }, { "type": "rRNA", "sequence": "contig_1", "start": 174522, "stop": 174630, "strand": "+", "gene": "rrf", "product": "5S ribosomal RNA", "coverage": 0.9159663865546218, "score": 72.8, "evalue": 5.1e-12, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00001", "KEGG:K01985", "SO:0000652" ], "nt": "TTTGGTGCTAATATCGCTGTGGAAACACCTGGAACCATCCCGAACCCAGCAGTTAAGCACAGTGGAGCTAAATGTAGGTAGTAATACTGAGAATAGGTAAGCACCAAGC", "id": "JPCNHPFPBM_39", "locus": "GCA000292505_00304" }, { "type": "cds", "sequence": "contig_1", "start": 174945, "stop": 175139, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGACCAAGGATGGCAAAGGGAAGGATAGTAGTGGGTGGAATAGTGAAGAAAATACGGCAGCAGGGGATGCGCCCAGTGTTACTGCAGGGAGGTAGTGCTTCTGGATCATTTAATAAATACCTCAACACCAAACAAGCGTTGGCAAGCATCGGCATCTTGTTTGAATGGAACAATGGCGAGAAATGTTGTTAG", "aa": "MGPRMAKGRIVVGGIVKKIRQQGMRPVLLQGGSASGSFNKYLNTKQALASIGILFEWNNGEKCC", "aa_hexdigest": "ac92962ca6334b6590966f7003f7c307", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6909.119699999999, "isoelectric_point": 10.392448616027831 }, "id": "JPCNHPFPBM_341", "locus": "GCA000292505_00305" }, { "type": "cds", "sequence": "contig_1", "start": 175332, "stop": 175493, "strand": "+", "frame": 3, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "RefSeq:WP_238532028.1", "SO:0001217", "UniParc:UPI001EE66E68", "UniRef:UniRef100_UPI001EE66E68", "UniRef:UniRef50_UPI001EE66E68", "UniRef:UniRef90_UPI001EE66E68" ], "nt": "ATGGAGGGGCAAGACGCCTTTTCGTTTGTTGTTCCTTATTCAATGAATCACACAAATAATGGTTCATCAGGACCCATTAAAACTGCTTATCCAGTGAAAAACACAGAGAAAGCAACTGTCAAGATCAATTCTTTGATCAACGCTACGCCGTTGAATAGTTAA", "aa": "MEGQDAFSFVVPYSMNHTNNGSSGPIKTAYPVKNTEKATVKINSLINATPLNS", "aa_hexdigest": "9f9335e93c93467ef4500e4e28022ff0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI001EE66E68", "ncbi_nrp_id": "WP_238532028.1", "uniref100_id": "UniRef100_UPI001EE66E68", "db_xrefs": [ "SO:0001217", "UniParc:UPI001EE66E68", "RefSeq:WP_238532028.1", "UniRef:UniRef100_UPI001EE66E68" ] }, "ips": { "uniref100_id": "UniRef100_UPI001EE66E68", "uniref90_id": "UniRef90_UPI001EE66E68" }, "psc": { "uniref90_id": "UniRef90_UPI001EE66E68", "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI001EE66E68" }, "pscc": { "uniref50_id": "UniRef50_UPI001EE66E68", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EE66E68" ], "product": "MgpC family cytadherence protein" }, "genes": [ "mgpC" ], "id": "JPCNHPFPBM_342", "locus": "GCA000292505_00306" }, { "type": "cds", "sequence": "contig_1", "start": 175793, "stop": 176083, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACCAACAGGATAAACTTGAATTTAACTTAACAAGATTAAAGGAGATAGATGTTGAAGATGGTAAGCAACTATTTGGGATAGAAACTAGTGTTTATAAACACTTTCAAAATGAAATTGCTCGTTTTTATAAACAAGTAAACAAACACTTTCGTGAAACAGGTAGTGAAAGTTTGTTTTTAGCTTTACCAGTTATTGAAGGGATTAATGAGTTTAACGATATCTTTCGAGCTCCATTACTTTATGTTGGAGTTAAACTCAAAGTTTCCCCACGCTTTGAACGTTTCTGA", "aa": "MNQQDKLEFNLTRLKEIDVEDGKQLFGIETSVYKHFQNEIARFYKQVNKHFRETGSESLFLALPVIEGINEFNDIFRAPLLYVGVKLKVSPRFERF", "aa_hexdigest": "f2df0dde154fc7de8d26cbbee3a24d31", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11369.886599999989, "isoelectric_point": 6.736182212829591 }, "id": "JPCNHPFPBM_343", "locus": "GCA000292505_00307" }, { "type": "cds", "sequence": "contig_1", "start": 176156, "stop": 177007, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12" ], "nt": "TTGTTTAAAAATAACTATGATACTACTAAGATAGATGTTAATGATGCTTTAAAGGTATTTAGTGAACTTGAATATGAGTTTAGAATGCCTTTAACTTCTGAATTGAAGAGTTTTAGTAAAAAAGCAAAGAGTGATTTTAATACTGAAAAACGAACTAACTATCTTATTAACAACGTTCTTTTGGGGATCTTTGATGTTAAGGGTGATCAGCTGTTCCAAAACTTTAATGAGATTCTAAACACTGATCCTGATGTATTAGATGAACTTCTAAAAGATAGAAGAGATCTGTTGTTAGAAAACCGGGAATTCCGTGAACAATTTGATTTAAAAGATACCTATCTCTTCAGTCACCTTGATATCTACCAACAGTATGCAGTTAAGCAAGCTTTACTTGGTGATTTAATTATTGAAGGCCCACCTGGCACAGGGAAATCTGAAACAATTGTTAATATCTTAGTTAACCTTGTTTTAAACAACAAAAAAGTATTGTTTGTTTCTGAAAAAGTAACTGCACTTGATGTTGTTTACAACCGTCTTGGTAGTTTTAAACACATCACACTTTTCAACGCTAGTGTTGCTGCTGAAAAGAAACGCTTTTATAACCAGTTTGCTGAGTTTGAAACTTATTTCACTACTTACTTTTCCAAGAAAGATTTGGATGCTACTTTACCAACATTTGAAGGTAAATGGGTGGATGATATTTTAGGGGCATTTCAAGCATTACAAGCTCTTTATGACACCAAGATAAATTCTGGTGAAAATCTGTTTAGTTTCAAAGAGATTGTCAGTAGCTTTCAGATGTTGGATGCTAGTTACATCAAGATTAAAGAATATGAACGTTTTGATGAGTGA", "aa": "MFKNNYDTTKIDVNDALKVFSELEYEFRMPLTSELKSFSKKAKSDFNTEKRTNYLINNVLLGIFDVKGDQLFQNFNEILNTDPDVLDELLKDRRDLLLENREFREQFDLKDTYLFSHLDIYQQYAVKQALLGDLIIEGPPGTGKSETIVNILVNLVLNNKKVLFVSEKVTALDVVYNRLGSFKHITLFNASVAAEKKRFYNQFAEFETYFTTYFSKKDLDATLPTFEGKWVDDILGAFQALQALYDTKINSGENLFSFKEIVSSFQMLDASYIKIKEYERFDE", "aa_hexdigest": "f1cac3d5d59b4552548bcaa48e0ffc50", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 73, "aa_cov": 0.22614840989399293, "hmm_cov": 1.0, "evalue": 1.2127830191600757e-09, "score": 33.38302230834961, "start": 118, "stop": 181 } ], "seq_stats": { "molecular_weight": 32967.99140000003, "isoelectric_point": 4.954166221618652 }, "id": "JPCNHPFPBM_344", "locus": "GCA000292505_00308" }, { "type": "cds", "sequence": "contig_1", "start": 177062, "stop": 177382, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAAAGAACTTAGTCAGCGCTGGAATGGTATTGATAATTTCTATCAGTTGCAATCGCTTTTAAACCAAACCAAAAAACGAAAGGTTTTAAACTATGTGTTGGAACACTTTGAACAGTTTAATACAGTTATCAGTCCTAAGCATGTTTTGTTCTACAAGCCTAGCAATAAATCACAATTGCTCTTAAAACAACTGAAACAGGATGTTGAACAATACACTAGTTTACAACGTTTCCAATCTCCTACTAAGTTTGAAACAATCAAGTTGAATTTCATCAACCAAGTTAATGAAAACCCAACCCCATGGTTCTTTTCTTGA", "aa": "MKKELSQRWNGIDNFYQLQSLLNQTKKRKVLNYVLEHFEQFNTVISPKHVLFYKPSNKSQLLLKQLKQDVEQYTSLQRFQSPTKFETIKLNFINQVNENPTPWFFS", "aa_hexdigest": "60f7feb5b772c56f979b4cfafbb6c1d6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12827.560899999988, "isoelectric_point": 9.776324272155762 }, "id": "JPCNHPFPBM_345", "locus": "GCA000292505_00309" }, { "type": "cds", "sequence": "contig_1", "start": 177410, "stop": 178102, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12" ], "nt": "TTGGAAAAACTCGTTAGTTTTGAGTCAAACATTATTAAAACAAAACAAGCTTATCTTAATGGGATTGAAAGCTATGTAGCAAGTTGTAAGAAACTGCTTAAAACAACTATTTTAAACAATTTTTTTCAGCTTTATCAAACTAATAAAGATGAACTATTGGAGATCTGCAGACAAGCAAAAAACCCAGTTTTAAAAGAGATTACTTGGTGGTTTAAAAAACATTTTGAACTCTTAAAAAAACTCTTTCCAGTGCACATTATGACCCTTGAGTCTGCAGCAACTCTAACCCCTAACCAACGTGGTTTGTATGACTATGTGGTTATTGATGAAGCTAGTCAAGTATTTCTAGAAAGAGCAATTCCTATCTTATTTAGAGCTGATAAGTACATTATTGCAGGGGACACTAAACAGTTAAAACCAGCTAACTTCTTCCAATCACGTGCTGAGTATGATGTTGATGAGGAGTTTGAAGATGGCAATATAGAAGCTGCTGTTCACTCCAGTTCTCTACTTCATTTTTTAAAGAACCGTTCAAGAATCTTAACTTTACTTAAGTTCCACTACCGCAGTGATTCAGCTGATCTGATTGCTTTTACTAATAACAGGATCTATGACAATGAATTAATCTTTATGAATAAAGCTAATGCTGATCAAAGGGTTTTTATTGTCCATGATGTAATAGATGGTATCTGA", "aa": "MEKLVSFESNIIKTKQAYLNGIESYVASCKKLLKTTILNNFFQLYQTNKDELLEICRQAKNPVLKEITWWFKKHFELLKKLFPVHIMTLESAATLTPNQRGLYDYVVIDEASQVFLERAIPILFRADKYIIAGDTKQLKPANFFQSRAEYDVDEEFEDGNIEAAVHSSSLLHFLKNRSRILTLLKFHYRSDSADLIAFTNNRIYDNELIFMNKANADQRVFIVHDVIDGI", "aa_hexdigest": "e6aee446c4281807e0d2828a137a8f41", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 54, "aa_cov": 0.23043478260869565, "hmm_cov": 1.0, "evalue": 3.0652098623299334e-10, "score": 35.340450286865234, "start": 88, "stop": 140 } ], "seq_stats": { "molecular_weight": 26793.49060000002, "isoelectric_point": 6.603235816955567 }, "id": "JPCNHPFPBM_346", "locus": "GCA000292505_00310" }, { "type": "cds", "sequence": "contig_1", "start": 178139, "stop": 178273, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAACGCTTAGAACAACTAACAACAACCAATGATTACAAAAAGTCACTTGGTGTAATTTGTTTCAATAAAAACCAAGCAGATCTAATTGAATACTTAATTGATAAACAAAACAATCCGCTACTTAACGAGTGA", "aa": "MQRLEQLTTTNDYKKSLGVICFNKNQADLIEYLIDKQNNPLLNE", "aa_hexdigest": "3284f53694d67ee22a658d358df2cd28", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5170.8263, "isoelectric_point": 5.145031547546386 }, "id": "JPCNHPFPBM_347", "locus": "GCA000292505_00311" }, { "type": "cds", "sequence": "contig_1", "start": 178316, "stop": 178894, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13087.12" ], "nt": "GTGAAAAACATTGAAAATGTGCAGGGTGATGAAAGGGATATCATCATCTTCTCTTTAGGTTATGATCGTTCAGTAAATAGTTATGGTCCTATTAGTAAGCAAGGTGGGGAAAACAGACTAAATGTAGCGATAACTAGAGCTAAACAAAGAATAGAACTGTTTAAGACAAATAGAGGGGAAGACTACAATGGTTTAAGCTCCAGTTCTTTGGGTAGTAAACTGTTAGTTGAATATCTACTTTATTGTGAAGCAATGGCTAAAAATCAGGGTGAGAAAATCACTTTTCAAGCGGTAAAAAGAAAAGAAACAAAAGCAAAGTATGAACTGGCAGTGGAAAATGATTTCTTCAACCAACTGCAGGCAATTTTTGGTGGAGAGTTTGAGATTAAACGTAACGTTAATGAAGGGGCTTACTTTTTCTCATTTGTCTTTTACTTTAATAATATCCCTTATCTTGCCATTGACTTTAATATCCCCATTCCCACTTCAAGAAAACAAGTTATGGAAGGGATTTTATACCGCGAACAGTTTCTCAAAAAACGCCAATGGAACCTAATTAACATCTGGATTGATGAGTGA", "aa": "MKNIENVQGDERDIIIFSLGYDRSVNSYGPISKQGGENRLNVAITRAKQRIELFKTNRGEDYNGLSSSSLGSKLLVEYLLYCEAMAKNQGEKITFQAVKRKETKAKYELAVENDFFNQLQAIFGGEFEIKRNVNEGAYFFSFVFYFNNIPYLAIDFNIPIPTSRKQVMEGILYREQFLKKRQWNLINIWIDE", "aa_hexdigest": "1457f84b849b54c1a02dc0eef28a2bde", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13087.12", "name": "AAA_12", "length": 50, "aa_cov": 0.2604166666666667, "hmm_cov": 0.94, "evalue": 4.909111900571236e-09, "score": 31.11225128173828, "start": 2, "stop": 51 } ], "seq_stats": { "molecular_weight": 22372.20070000001, "isoelectric_point": 8.510100746154787 }, "id": "JPCNHPFPBM_348", "locus": "GCA000292505_00312" }, { "type": "cds", "sequence": "contig_1", "start": 179050, "stop": 179658, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08529.16" ], "nt": "ATGAATGAATTAAACCAACCCTTACTTGCTATTATTAAAAATGTCGCTAAAACCAAAAACCTTTCTATAGAAGAGGTGGTTTTTTGTTTGAAAACAGCTTTAGAACAAGCCTATAAAAAACACCTTAACTTTGTTAATGTTGAAGTTAACATTGACTTTGATAAGGGGATTATTAATGTTGAACAACTCTTTAATGTTGTCAGTGATGAAAATGAAGATTATGATGACTTTCTTGAAATCCCTTTACAAGCAGCTAACAAAATAAGCAGTTCATTGCAATTAGGTGATGTGTTGCGTAAACCAATCCCCTTAAAGAACATCAGTAGTGATCTTATAAATAAGATGATTGCTATCTTTAACCAAAAGATTAGTGAAACAAACTTTAAAGCAGTAATGAGCGAGTTTAGTAGTGAGGTTGGGGAAGTGATTGAAGCGAAAGTTGAAGATATTGATACTAACAAAGAAGGTGGTTTAAAGGGTTATATTATTAACCTTGAAACTACAAAGGGTTATATCTCCAAGCGGGAATTGTCAAAAGGGGAGCGCTTAGAGATAGGTAAAAAATACCTCTTTGTTATCAAAGAAATCCAACGGCAAGCATCGTTATGA", "aa": "MNELNQPLLAIIKNVAKTKNLSIEEVVFCLKTALEQAYKKHLNFVNVEVNIDFDKGIINVEQLFNVVSDENEDYDDFLEIPLQAANKISSSLQLGDVLRKPIPLKNISSDLINKMIAIFNQKISETNFKAVMSEFSSEVGEVIEAKVEDIDTNKEGGLKGYIINLETTKGYISKRELSKGERLEIGKKYLFVIKEIQRQASL", "aa_hexdigest": "8bd5fc265a147c2797533f6e8d755ad3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08529.16", "name": "NusA_N", "length": 121, "aa_cov": 0.5891089108910891, "hmm_cov": 0.9504132231404959, "evalue": 3.0307044585038332e-21, "score": 70.97576904296875, "start": 8, "stop": 126 } ], "seq_stats": { "molecular_weight": 22855.04380000001, "isoelectric_point": 5.162878990173339 }, "id": "JPCNHPFPBM_349", "locus": "GCA000292505_00313" }, { "type": "cds", "sequence": "contig_1", "start": 179701, "stop": 179907, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13184.12" ], "nt": "TTGTTAACTTCAAATACTCCAGAAATTGAAAATGGCACGATTGTAATCAAAAAGATTGAACGTTCCCCAGGAGTGAAATCAAAGATAGCAGTTATCTCCAATGATCCTGCAGTTGACCCAGTTGCTGCTATCTTAGGACCTAAGGGTGAGAAGATTAGGGGGATTAGTGAGGAATTTAATGGTGAGATTATTGACATTGTTTTTTGA", "aa": "MLTSNTPEIENGTIVIKKIERSPGVKSKIAVISNDPAVDPVAAILGPKGEKIRGISEEFNGEIIDIVF", "aa_hexdigest": "610aa7a4b1e2d4441bf835589b588956", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13184.12", "name": "KH_5", "length": 45, "aa_cov": 0.6617647058823529, "hmm_cov": 1.0, "evalue": 9.333057757682073e-14, "score": 46.4936408996582, "start": 23, "stop": 67 } ], "seq_stats": { "molecular_weight": 7260.320699999999, "isoelectric_point": 4.962578392028808 }, "id": "JPCNHPFPBM_350", "locus": "GCA000292505_00314" }, { "type": "cds", "sequence": "contig_1", "start": 179974, "stop": 180090, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAGGATGATGAACGTGATACTAGTATTGAAGTTGTTGTACCTGCAAACCAAATTGCTAATGTTTTTGGTTTTAAGGGTGTAAACATTAGGTTAATTAGTAATTTAACAGGTTGA", "aa": "MQDDERDTSIEVVVPANQIANVFGFKGVNIRLISNLTG", "aa_hexdigest": "2220e13df8ecc2c719c17cb87073ef0f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4161.6494999999995, "isoelectric_point": 4.436874580383301 }, "id": "JPCNHPFPBM_351", "locus": "GCA000292505_00315" }, { "type": "cds", "sequence": "contig_1", "start": 180172, "stop": 180570, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGGCATTAAAAAAAGAAGGGAAAAGATCATTAGTAATGATGCTACTGATAAAGTCTTTTACACTTCTAAAGACAATGTGATAGATGATGAGATTATTGTTGATTTAGCTAAAGATCTAATGGTTGATAATAAACAAAAACAACCTGAGCAAGTTGCAAAACAAGTTGTTGAAAAACCACAATTAGAAAAACAAGTTACTCCAAAAGAAAAAGAGAAAGTTCAACCAAAAGCTAAGGTTCATTCTAATAGCCATTCCAAAAAACCAGTTAAAACTAATCAGGTTTTTTCTATCACTGTTGATGCTAGTGATAAGAATCTTAAAAAAGATCAAGTTGATAATAACCAAACAAACCCCCAAACAAAACAAACATTTGATAGCTTTGATGATCTTTAA", "aa": "MFGIKKRREKIISNDATDKVFYTSKDNVIDDEIIVDLAKDLMVDNKQKQPEQVAKQVVEKPQLEKQVTPKEKEKVQPKAKVHSNSHSKKPVKTNQVFSITVDASDKNLKKDQVDNNQTNPQTKQTFDSFDDL", "aa_hexdigest": "6384213a6ae7970a98ffeae51e8c1738", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15154.951999999983, "isoelectric_point": 8.961314964294434 }, "id": "JPCNHPFPBM_352", "locus": "GCA000292505_00316" }, { "type": "cds", "sequence": "contig_1", "start": 180570, "stop": 180842, "strand": "+", "frame": 3, "gene": "ylxR", "product": "putative protein MG141-1", "db_xrefs": [ "COG:AJ", "COG:COG2740", "RefSeq:WP_014894591.1", "SO:0001217", "UniParc:UPI00027B3662", "UniRef:UniRef100_UPI00027B3662", "UniRef:UniRef50_Q2MHT0", "UniRef:UniRef90_Q2MHT0" ], "nt": "ATGCAACTAATAACAAGACTTTGTTTATTAACAAGAAAACATTTTGTTAAAAGAGAACTTTTACGTCTTGTAAAATTAGACAACCAACTTGAAATTGATCTTAATCAAAATCTCAAGGGCAGGGGTTATTATTTGAGTGTTTTTGGTTTAAAGATAGATAAAAAACACCTCAAAGCTGTAGTTGAAAAACACCTTAAGGTTAGTTGTAATGATGCAAAGCTTACTGCAATGATTACCGCCTTACAACAATTAGCACAAGATGAAAAAAAATAG", "aa": "MQLITRLCLLTRKHFVKRELLRLVKLDNQLEIDLNQNLKGRGYYLSVFGLKIDKKHLKAVVEKHLKVSCNDAKLTAMITALQQLAQDEKK", "aa_hexdigest": "9a896bb5220252cc25dcfb1f253add50", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3662", "ncbi_nrp_id": "WP_014894591.1", "uniref100_id": "UniRef100_UPI00027B3662", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3662", "RefSeq:WP_014894591.1", "UniRef:UniRef100_UPI00027B3662" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3662", "uniref90_id": "UniRef90_Q2MHT0" }, "psc": { "uniref90_id": "UniRef90_Q2MHT0", "gene": "YlxR", "product": "Uncharacterized protein MG141.1", "uniref50_id": "UniRef50_Q2MHT0", "cog_id": "COG2740", "cog_category": "AJ" }, "pscc": { "uniref50_id": "UniRef50_Q2MHT0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q2MHT0" ], "product": "Uncharacterized protein MG141.1" }, "genes": [ "ylxR" ], "id": "JPCNHPFPBM_353", "locus": "GCA000292505_00317" }, { "type": "cds", "sequence": "contig_1", "start": 180829, "stop": 182688, "strand": "+", "frame": 1, "gene": "infB", "product": "translation initiation factor IF-2", "db_xrefs": [ "COG:COG0532", "COG:J", "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0006413", "KEGG:K02519", "RefSeq:WP_014894592.1", "SO:0001217", "UniParc:UPI00027B3663", "UniRef:UniRef100_UPI00027B3663", "UniRef:UniRef50_P47388", "UniRef:UniRef90_P47388" ], "nt": "ATGAAAAAAAATAGAGCTTTCAACCAGGTTAAAAAAATAAAGTTTGATGGTAGGATTAAAACCAGTGCCAAACACCAGTTACGTAATGTTAAAACCGGGGTTAAAGATGGTGTTTTTATCTATAAAGGTCCTTTAACTGTTAGTGAGTTTGCAAGTAAAACTAATATCGCTGTTGCTAATATTATCAAGCACTTTTTTTTAAATGGTTTGGCACTAACAGTTAATTCAGTTTTAACAAATGAACAGTTAGCAGATGCATGTGTTAACTTTGGGTTTGACTTTAAGATGGAAACTGAAGTTACCCATGAAAATATTGTAGCTAACATCCAGTTTGAAGATAGTGATGATTTATTGCAACCAAGACCACCTATTGTTACTATCATGGGTCATGTTGATCATGGTAAAACTTCGCTTTTAGACACAATTAGAAAAACTAATGTAACTGCTAAGGAGTTTGGCGGAATTACCCAAAAAATTGGTGCTTATCAGGTGAAAAATCACCAAAATAAAACGATTACTTTTATTGATACTCCTGGGCATGAAGCATTTACTTTAATGCGTGCAAGGGGTGCGAAAGTAACTGATATTGTGGTGTTGGTTGTGGCAGCGGATGATGGGATTAAAAAGCAAACAGAGGAAGCAATTAGCCATGCTAAGAGTGCTAACACTCCTATCATTGTTTTTATTAACAAGATGGATAAACCAACTGCTAACCCTGATCTGGTGATCCAACAACTCAATAAGTTTGATTTAGTTCCTGAGGCTTGGGGTGGGAAAACTATCTTTGTAATGGGTAGTGCTTTAACTGGTCAAGGGATTAATGAGTTGCTTGATAATATCTTGTTGCTAGGGGAAGTGGAGGGTTATCAAGCTAACTATAATGCCCATTCATCTGGTTATGCAATTGAAGTACAAACTTCAAAGGGACTTGGCCCTATTGCCAATGTCATTGTAAAAAGGGGTACTTTAAAGTTAGGTGACATTGTGGTGTTAGGGCCTGCATATGGAAGAGTTAGAACGATGCATGATGAAAATGGTAATAGCTTAAAACAAGCAACCCCTTCAAAACCTGTGCAGATCTCAGGGTTTGACATTATGCCTGTTGCTGGGGAAAAGTTCATTGTTTTTGATGATGAGAAGGATGCAAAGTTAATTGCTAACAAGTTTAAAGAACAACAAAAACAAAAAGCTAACAACTTAACAGTTAATCAAACCTTAAAAGAACAGATTAAAAACAAGGAAATTAAGATATTAAATTTGATCTTTAAAGCAGATAGTGATGGTTCATTGCAAGCTATTAAACAAGCAGTTGAAAACATTAATGTTGCTAAGATCTCACTTAGTATCATCCATGCTGCAGTGGGGCAGATATCAGAGAGTGATATTATGCTAGCAAAAGCATCAGGGGCTTTATTGTTTAGTTTAAACTTAGGTTTGAGTCAAACTGTAAAAAACATTGCTAGTTTACAAGGGGTAAAATTAGAAGTTCACTACCATATCCCTAAACTAGCAGAGGAGATTGAAAACATCTTAAAAGGTCAATTAGATCCTGTTTATGAAGAGATTGAAATAGGTAAAGCGGAAGTTTTACAACTCTGGTTCCACTCTAAAATCGGTAATATTGCAGGAACCATTGTTAAATCAGGAAAGATAAAAAGAGGGAATTTATGTAAGTTATTCAGAGATAAAGAGATTATCTTTGAAGGCAGAATTGACTCTTTAAAAAATGAAAAAACGCCTGTTAATTTAATAGAAACAGGGAAGAATTGTGGGATAGTTATTAATGGTTGCAATGATATTAAGATTGGTGATATCATTGTTGCTTATGAAAAGCAGATAGTTAAAGATGGCAAGCTATAG", "aa": "MKKNRAFNQVKKIKFDGRIKTSAKHQLRNVKTGVKDGVFIYKGPLTVSEFASKTNIAVANIIKHFFLNGLALTVNSVLTNEQLADACVNFGFDFKMETEVTHENIVANIQFEDSDDLLQPRPPIVTIMGHVDHGKTSLLDTIRKTNVTAKEFGGITQKIGAYQVKNHQNKTITFIDTPGHEAFTLMRARGAKVTDIVVLVVAADDGIKKQTEEAISHAKSANTPIIVFINKMDKPTANPDLVIQQLNKFDLVPEAWGGKTIFVMGSALTGQGINELLDNILLLGEVEGYQANYNAHSSGYAIEVQTSKGLGPIANVIVKRGTLKLGDIVVLGPAYGRVRTMHDENGNSLKQATPSKPVQISGFDIMPVAGEKFIVFDDEKDAKLIANKFKEQQKQKANNLTVNQTLKEQIKNKEIKILNLIFKADSDGSLQAIKQAVENINVAKISLSIIHAAVGQISESDIMLAKASGALLFSLNLGLSQTVKNIASLQGVKLEVHYHIPKLAEEIENILKGQLDPVYEEIEIGKAEVLQLWFHSKIGNIAGTIVKSGKIKRGNLCKLFRDKEIIFEGRIDSLKNEKTPVNLIETGKNCGIVINGCNDIKIGDIIVAYEKQIVKDGKL", "aa_hexdigest": "8fe5e1ef6f3d7b08c3345c36ea041dca", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3663", "ncbi_nrp_id": "WP_014894592.1", "uniref100_id": "UniRef100_UPI00027B3663", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3663", "RefSeq:WP_014894592.1", "UniRef:UniRef100_UPI00027B3663" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3663", "uniref90_id": "UniRef90_P47388" }, "psc": { "uniref90_id": "UniRef90_P47388", "gene": "infB", "product": "translation initiation factor IF-2", "uniref50_id": "UniRef50_P47388", "cog_id": "COG0532", "cog_category": "J", "kegg_orthology_id": "K02519", "go_ids": [ "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_P47388", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47388" ], "product": "Translation initiation factor IF-2" }, "genes": [ "infB" ], "id": "JPCNHPFPBM_354", "locus": "GCA000292505_00318" }, { "type": "cds", "sequence": "contig_1", "start": 182675, "stop": 183013, "strand": "+", "frame": 2, "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "db_xrefs": [ "COG:COG0858", "COG:J", "GO:0005829", "GO:0006364", "GO:0030490", "GO:0042254", "GO:0043024", "KEGG:K02834", "RefSeq:WP_009885829.1", "SO:0001217", "UniParc:UPI00001331AF", "UniRef:UniRef100_P47389", "UniRef:UniRef50_P47389", "UniRef:UniRef90_P47389" ], "nt": "ATGGCAAGCTATAGAAAACAACGGATTGAAAATGATATCATCCGCTTAATTAATCGCACGATTATTAATGAGATCTATGATCCTGTTGTTAAGTTAGGTCATGTTAGCCATGTGAAGTTATCAGCTGATTTTTTTCATGCAGTAGTTTATCTTGATTGTTATGATCGTAGTCAGATTCAAACTGTAGTTAATGCTTTTAAAAAGGCTCAGGGAGTTTTTAGTCAAATGTTAGCACAAAATTTGTACCTAGCTAAAAGTGTAAAACTCCACTTTGTGAAGGATGATGCAATTGACAATGCTTTGAAAATAGAACAGATAATTAACTCTTTGAAAAACTAA", "aa": "MASYRKQRIENDIIRLINRTIINEIYDPVVKLGHVSHVKLSADFFHAVVYLDCYDRSQIQTVVNAFKKAQGVFSQMLAQNLYLAKSVKLHFVKDDAIDNALKIEQIINSLKN", "aa_hexdigest": "4500bbaa521ddaacfdfd8700f355eab2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001331AF", "ncbi_nrp_id": "WP_009885829.1", "uniref100_id": "UniRef100_P47389", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001331AF", "RefSeq:WP_009885829.1", "UniRef:UniRef100_P47389" ] }, "ips": { "uniref100_id": "UniRef100_P47389", "uniref90_id": "UniRef90_P47389" }, "psc": { "uniref90_id": "UniRef90_P47389", "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "uniref50_id": "UniRef50_P47389", "cog_id": "COG0858", "cog_category": "J", "kegg_orthology_id": "K02834", "go_ids": [ "GO:0005829", "GO:0006364", "GO:0030490", "GO:0042254", "GO:0043024" ] }, "pscc": { "uniref50_id": "UniRef50_P47389", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47389" ], "product": "Ribosome-binding factor A" }, "genes": [ "rbfA" ], "id": "JPCNHPFPBM_355", "locus": "GCA000292505_00319" }, { "type": "cds", "sequence": "contig_1", "start": 183038, "stop": 183466, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MG144", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47390", "UniRef:UniRef90_P47390" ], "nt": "ATGGACCCACAAAACAAGTCCCCAAAACCACCAGTTAAATCAACTAGATTGGTTGTCAAAAAACAACCAGCAGGTGTTGTTTTTCCCAAATTGAGTATTCCTGTTAATGATTTTGAAAAAACAGTTACTTTAACAAGAGCACAAAAAAAGGAAGCTAAACTTCTCAAAAAAGCCCAAAGGAAAGCTAATAAGCTTAATAATAAACAAGATAGTATCTTTTTCAATTCTGCTTCAGTAGGTGACACTAATAACACCATTCTTCCTCCTGGTGTTAAAAATCAAGCTGATAATAAAACTAACCGTTTTAGTAAATTTATTAGTTTTTTCACTTCTTCAAAAAACAAACAACCAGATGAAATAACAGAAAGGTTAGTTGATGATCCTACTGTTAAAAACCGTTTTAGTGCTTTTAATAAGAAGCTTATTTGA", "aa": "MDPQNKSPKPPVKSTRLVVKKQPAGVVFPKLSIPVNDFEKTVTLTRAQKKEAKLLKKAQRKANKLNNKQDSIFFNSASVGDTNNTILPPGVKNQADNKTNRFSKFISFFTSSKNKQPDEITERLVDDPTVKNRFSAFNKKLI", "aa_hexdigest": "06199abca5edc8e0e8d3e76731ead0eb", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 183038, "stop": 183769, "observations": { "mutation": [ 183464, 183638, 183683 ], "stop": [ 183464, 183638, 183683 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47390", "query_cov": 1.0, "subject_cov": 0.5071428571428571, "identity": 1.0, "score": 271.0, "evalue": 3.89e-90, "gene_start": 1, "gene_end": 142, "reference_start": 1, "reference_end": 142, "reference_sequence": "MDPQNKSPKPPVKSTRLVVKKQPAGVVFPKLSIPVNDFEKTVTLTRAQKKEAKLLKKAQRKANKLNNKQDSIFFNSASVGDTNNTILPPGVKNQADNKTNRFSKFISFFTSSKNKQPDEITERLVDDPTVKNRFSAFNKKLIWVLKDKKLRARAWKIVGYTNLVIVAFFAGLLAVMNKFITLPSVEYPAIALQLPINNALWGISIFVISIVTLPFWTMFILFLMGVKDVRTSRSIHYFIWIVLIINVVLLLVSCLLMIAAYAHLDGYNIWRNLESLNPNN" }, "paralog": false, "identity": 0.9876543209876543, "score": 465.0, "evalue": 5.49e-169, "description": "Internal stop codon at 183464, 183638, 183683. Nonsense mutation around 183464, 183638, 183683.", "psc": { "uniref90_id": "UniRef90_P47390", "product": "Uncharacterized protein MG144", "uniref50_id": "UniRef50_P47390", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47390", "UniRef:UniRef50_P47390", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47390", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47390" ], "product": "Uncharacterized protein MG144" } }, "id": "JPCNHPFPBM_356", "locus": "GCA000292505_00320" }, { "type": "cds", "sequence": "contig_1", "start": 183533, "stop": 183640, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCATTTTTTGCTGGACTTTTAGCAGTGATGAATAAGTTCATCACCCTTCCCTCAGTTGAATATCCTGCTATTGCTTTACAACTCCCTATTAACAATGCATTATGA", "aa": "MAFFAGLLAVMNKFITLPSVEYPAIALQLPINNAL", "aa_hexdigest": "b257ed08b460d0971813e7182eda9929", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3792.5511000000006, "isoelectric_point": 5.752754020690918 }, "id": "JPCNHPFPBM_357", "locus": "GCA000292505_00321" }, { "type": "cds", "sequence": "contig_1", "start": 183689, "stop": 183847, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATCTTGTTTTTAATGGGAGTAAAGGATGTGAGAACTTCCCGTTCTATCCATTATTTTATCTGGATAGTGTTAATTATTAATGTAGTTTTATTACTAGTTAGTTGCTTGTTGATGATTGCTGCTTATGCCCATCTTGATGGTTATAACATCTGA", "aa": "MFILFLMGVKDVRTSRSIHYFIWIVLIINVVLLLVSCLLMIAAYAHLDGYNI", "aa_hexdigest": "96b80d41985d832101b1e57399461bd2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6013.336699999999, "isoelectric_point": 7.959798622131348 }, "id": "JPCNHPFPBM_358", "locus": "GCA000292505_00322" }, { "type": "cds", "sequence": "contig_1", "start": 183880, "stop": 184689, "strand": "+", "frame": 1, "gene": "ribF", "product": "riboflavin biosynthesis protein RibF", "db_xrefs": [ "COG:COG0196", "COG:H", "EC:2.7.1.26", "EC:2.7.7.2", "GO:0003919", "GO:0005524", "GO:0005737", "GO:0006747", "GO:0006771", "GO:0008531", "GO:0009231", "GO:0009398", "KEGG:K11753", "RefSeq:WP_009885831.1", "SO:0001217", "UniParc:UPI00001338E0", "UniRef:UniRef100_P47391", "UniRef:UniRef50_P47391", "UniRef:UniRef90_P47391" ], "nt": "ATGAAATCACTCTTTATTGGTTATTTTGATGGGTTACATCAAGGTCATCTATTTTTAAAGCAGAACAGTAAGTTTGAACCAATGGTGTTATTAATTGATAACCCACCTTTAAAACAAACCAACTGGCTTTATGATTTACAACAACGGGTTGCACAAATAAAAACTTACTTGAAAGCAACTGTAGAAGTATTTGATGTTGCCAAACATAACATGAATGCACTTAGTTTTTTTGAACAACAGATTAAAAGATTGAATTGTGATGAAATTATTGTTGGTACAGATTGGCATTTTGGTAATGATCATAAGGATGGGATCTGGTTAAAGAAACTGTTTAAAAATACTGTTATTGTTAATAAAACAAACCTATCAAGTAGTGTTATCCGTAACTATCTAACTAATAATGAACTTGAAAAAGCTAACCAACTTTTAGTGGAACCTTATTATAGAGTGGGCACAGTAGTACATGGTTTAAAAAAGGCAAGGTTACTTGGTTTTCCAACTGCTAACATTGTTATGGATAACCACTTATTGACTTTAAATAAGGGGAGCTATATAGTAAGAGTTTTATTAAATAACCAAACCTTTTATGGGATTGGTTTTATTAGCCAAAAGGATCAGGATTTGGTGTGTGAAACCCATATCTTTAACTTTAATAATGAGATTTATGGTTCACTGGTCAAATTTACACTGTTAAAGTTCATTAGAACAATTAGTAAGTTTTCCAGTCAAGCAGCTTTGCAAAAAGCAATTCAAAGTGATGCTAACTTTGCTTTAAAGTGGTTGGAAAACCAAAATTTAGATAAAATTTAA", "aa": "MKSLFIGYFDGLHQGHLFLKQNSKFEPMVLLIDNPPLKQTNWLYDLQQRVAQIKTYLKATVEVFDVAKHNMNALSFFEQQIKRLNCDEIIVGTDWHFGNDHKDGIWLKKLFKNTVIVNKTNLSSSVIRNYLTNNELEKANQLLVEPYYRVGTVVHGLKKARLLGFPTANIVMDNHLLTLNKGSYIVRVLLNNQTFYGIGFISQKDQDLVCETHIFNFNNEIYGSLVKFTLLKFIRTISKFSSQAALQKAIQSDANFALKWLENQNLDKI", "aa_hexdigest": "1b90d804a9a9d34cfb6cb35101a8d0e9", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001338E0", "ncbi_nrp_id": "WP_009885831.1", "uniref100_id": "UniRef100_P47391", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001338E0", "RefSeq:WP_009885831.1", "UniRef:UniRef100_P47391" ] }, "ips": { "uniref100_id": "UniRef100_P47391", "uniref90_id": "UniRef90_P47391" }, "psc": { "uniref90_id": "UniRef90_P47391", "gene": "ribF", "product": "riboflavin biosynthesis protein RibF", "ec_ids": [ "2.7.1.26", "2.7.7.2" ], "uniref50_id": "UniRef50_P47391", "cog_id": "COG0196", "cog_category": "H", "kegg_orthology_id": "K11753", "go_ids": [ "GO:0003919", "GO:0005524", "GO:0005737", "GO:0006747", "GO:0006771", "GO:0008531", "GO:0009231", "GO:0009398" ] }, "pscc": { "uniref50_id": "UniRef50_P47391", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47391" ], "product": "Bifunctional riboflavin kinase/FMN adenylyltransferase" }, "genes": [ "ribF" ], "id": "JPCNHPFPBM_359", "locus": "GCA000292505_00323" }, { "type": "cds", "sequence": "contig_1", "start": 184702, "stop": 184827, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACAGTGCCCCCAGTGGTTTAACTTTAACTGTTATTATCCTTAGCATCATTCTGCTTGCTTTTATCAGCACAGTTGTATCAGCTTATGAAACAGCAATCACTTCTTTAACCCCTTACAGGTGA", "aa": "MDSAPSGLTLTVIILSIILLAFISTVVSAYETAITSLTPYR", "aa_hexdigest": "aa09da1cac1cae4958c9e0a811a6736d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4343.083299999999, "isoelectric_point": 4.369065666198731 }, "id": "JPCNHPFPBM_360", "locus": "GCA000292505_00324" }, { "type": "cds", "sequence": "contig_1", "start": 184951, "stop": 185151, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTCTAACATCCTTTTTTTAGCGCTAGAACAAACAATTAAAAATGAGCTTTTATCAAGTGTTTTAAATTTGGTAGTTAGTGGGGTTTTAATCGTCTCTTTTTGTGAAATTCTACCCAAAACTTTGGGCAGAATTAATGTGATTAGAACCCTGGTTCTATTTGCTTATTTGGTTTATTTTTTTTATCTGATCTTTTGA", "aa": "MVSNILFLALEQTIKNELLSSVLNLVVSGVLIVSFCEILPKTLGRINVIRTLVLFAYLVYFFYLIF", "aa_hexdigest": "704535b5478442b3e2e21541772bb6e1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7526.074599999995, "isoelectric_point": 7.899520683288574 }, "id": "JPCNHPFPBM_361", "locus": "GCA000292505_00325" }, { "type": "cds", "sequence": "contig_1", "start": 185347, "stop": 185742, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00571.33" ], "nt": "GTGGCTTATTGTTATCTTAATGACAGTATTAACTTGATTGCCAAGCAGTTTTTACAAGGGCAGTTTTCCAGAATGCCAGTAGTAGATAAAACTACTAATAAGATAGTTGGTTTTATCCATTTAAAGGATTTTTTTACAGCTAAAGAAGCAAACCCTAAGTCACTTGATTTAAACCAGTTGCTTTATCCAGTTGTTTTAGTTCAAGATTCCACCCCCATCAAACAAGCACTAAGACAGATGCGTTTAAACAGAGCACATTTAGCAGTTGTTAATGATAAACATGAAAAAACAATAGGGATTGTTTCTATGGAGGATATTATTGAAGAGTTGGTGGGTGAAATCTATGATGAACATGATGATATCCAACCGATCCAAGTATTGGATGAAAATGTTTGA", "aa": "MAYCYLNDSINLIAKQFLQGQFSRMPVVDKTTNKIVGFIHLKDFFTAKEANPKSLDLNQLLYPVVLVQDSTPIKQALRQMRLNRAHLAVVNDKHEKTIGIVSMEDIIEELVGEIYDEHDDIQPIQVLDENV", "aa_hexdigest": "592192f3d29fcbca1e16bb514e5772fc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00571.33", "name": "CBS", "length": 50, "aa_cov": 0.37404580152671757, "hmm_cov": 0.98, "evalue": 1.234901125549325e-19, "score": 65.50303649902344, "start": 63, "stop": 111 } ], "seq_stats": { "molecular_weight": 15010.11379999999, "isoelectric_point": 5.084725379943847 }, "id": "JPCNHPFPBM_362", "locus": "GCA000292505_00326" }, { "type": "cds", "sequence": "contig_1", "start": 185800, "stop": 185976, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAAAATCAAAAAATATTACTATCCAGCATTATCTCGCTTCACTAGATAATGATAGTTTTGCTTGCCAAAATAAACTTGACACCCCCTTATTTAGTGTTGAAGTGATAGCTGATAGCGAAGATAAAACCAAAATTCTTTACGAAATTAGAAAGAAGAGTGATGTTATTGCTTAG", "aa": "MVKSKNITIQHYLASLDNDSFACQNKLDTPLFSVEVIADSEDKTKILYEIRKKSDVIA", "aa_hexdigest": "623a728d4b378e88c1b6ba185ec7bab2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6588.471799999999, "isoelectric_point": 5.628106117248534 }, "id": "JPCNHPFPBM_363", "locus": "GCA000292505_00327" }, { "type": "cds", "sequence": "contig_1", "start": 185963, "stop": 186616, "strand": "+", "frame": 2, "gene": null, "product": "MPN160 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47393", "UniRef:UniRef90_P47393" ], "nt": "ATGTTATTGCTTAGAGCATTATTTTTAGAGTTGAAAACCAACAAAAATTGTAAAGCTTTACTTTTGTTATTAATCCCCTTACTAGTTGGTTTAACCTTGATAATCTATGGGATTGTCTTATTTTCCACTGAAGGGGTAATTGATCATGGTGATCATAACCACTTAAGAGCAAGGTTTCAACTCACTTTAGAGGAGATTATTGTTTTTGTTGTTGGTAGTATTATCTTGTTTTTTACTTTAGCTAGCTTTTGTGTGAGTTGCTTTATGTTAATGAGAAGTCCTAAGCAAAAACAGCTAGAGGTTGATCATGCTAATAAAACTAATTTAAAACCAAAAGCAATAGTTAATTGTGATCTTTTTCAGTTGGGTGATTACTGTGTGTTTACATTTAAAAAACTTAGCTTTAAACAACGGTTTAAGCAAGATTTTTTTGCTAGAAGTAAGTTTTCGTTTCGCAGTGAACTGTATCGTCTTTGTTTGGTGGGAGTGTTAATTGCTCTCAATTTAGCGTTAAGTTTGATTGAAATTCCTGGGATAGTTTTACCTTGGGGTAGTTCGATCCAATTCCGTTTTTTTAATACGGCTATCTTGTTTATTGCTGTTAGGTTGGTGGGATTATTATCTACTTCTTTAGTGGCTTTAATCACCCCCTGA", "aa": "MLLLRALFLELKTNKNCKALLLLLIPLLVGLTLIIYGIVLFSTEGVIDHGDHNHLRARFQLTLEEIIVFVVGSIILFFTLASFCVSCFMLMRSPKQKQLEVDHANKTNLKPKAIVNCDLFQLGDYCVFTFKKLSFKQRFKQDFFARSKFSFRSELYRLCLVGVLIALNLALSLIEIPGIVLPWGSSIQFRFFNTAILFIAVRLVGLLSTSLVALITP", "aa_hexdigest": "c74e5bac676bd9d08eae815fbff10e3a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 185963, "stop": 186919, "observations": { "mutation": [ 186614, 186803 ], "stop": [ 186614, 186803 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47393", "query_cov": 1.0, "subject_cov": 0.5786666666666667, "identity": 1.0, "score": 401.0, "evalue": 1.17e-138, "gene_start": 1, "gene_end": 217, "reference_start": 1, "reference_end": 217, "reference_sequence": "MLLLRALFLELKTNKNCKALLLLLIPLLVGLTLIIYGIVLFSTEGVIDHGDHNHLRARFQLTLEEIIVFVVGSIILFFTLASFCVSCFMLMRSPKQKQLEVDHANKTNLKPKAIVNCDLFQLGDYCVFTFKKLSFKQRFKQDFFARSKFSFRSELYRLCLVGVLIALNLALSLIEIPGIVLPWGSSIQFRFFNTAILFIAVRLVGLLSTSLVALITPWLHLLIHPIHTPISSLFYMVNDFLVLWIFYFFFFHLFKAEVIQTTTVVDNKPFSQLVNTKKTKWTKFFSLLVISFLCGFIEGLGFYFGYFLILGNVSSLGLKIYYDGLQQRDLINSSNVLFFLMTTTAIFSIKYIFEMLFFFSVEKNVVNIANHFGLY" }, "paralog": false, "identity": 0.9937106918238994, "score": 620.0, "evalue": 2.09e-227, "description": "Internal stop codon at 186614, 186803. Nonsense mutation around 186614, 186803.", "psc": { "uniref90_id": "UniRef90_P47393", "product": "MPN160 family protein", "uniref50_id": "UniRef50_P47393", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47393", "UniRef:UniRef50_P47393", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47393", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47393" ], "product": "Uncharacterized protein MG147" } }, "id": "JPCNHPFPBM_364", "locus": "GCA000292505_00328" }, { "type": "cds", "sequence": "contig_1", "start": 186839, "stop": 187090, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTGTGGCTTTATTGAGGGGTTAGGTTTTTACTTTGGTTATTTTTTAATCCTTGGTAATGTTTCTAGTTTAGGATTGAAGATCTATTATGATGGGTTACAACAACGTGATCTGATTAACAGTAGTAATGTTTTGTTCTTTTTAATGACAACAACTGCTATCTTCAGTATTAAATACATCTTTGAGATGTTATTTTTCTTTAGTGTTGAAAAGAATGTAGTTAATATTGCTAACCACTTTGGTTTGTATTAA", "aa": "MCGFIEGLGFYFGYFLILGNVSSLGLKIYYDGLQQRDLINSSNVLFFLMTTTAIFSIKYIFEMLFFFSVEKNVVNIANHFGLY", "aa_hexdigest": "6ef975fa2b9796bce3755a4651fb6480", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9566.094799999993, "isoelectric_point": 5.538129997253416 }, "id": "JPCNHPFPBM_365", "locus": "GCA000292505_00329" }, { "type": "cds", "sequence": "contig_1", "start": 187129, "stop": 187281, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGTATGTAAAAGTACAGATCATCAATAAATCAACTATTGAACTGTTAGAGGATGCTAAAAAAGGGGAAAAAATTAATTTAGATTTAATTAACCAAGTTGATCAGACCAACATCCTCAATACGATCACTACTAACCAAAAATTAGCATGA", "aa": "MKYVKVQIINKSTIELLEDAKKGEKINLDLINQVDQTNILNTITTNQKLA", "aa_hexdigest": "e403c62e7b38e41ae685bec0fd92ba00", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5699.572900000002, "isoelectric_point": 8.034453010559083 }, "id": "JPCNHPFPBM_366", "locus": "GCA000292505_00330" }, { "type": "cds", "sequence": "contig_1", "start": 187366, "stop": 187620, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAATGACAAAGAGCAAGCATTGTTATTAAAAACCAAATTAGAATTACAAAACCAGTTTCAAAAGCAGATAGAGAACTATCTCAATGAGATTAACAAGCTCAAGTTAATCAATAAAGAGCTTGAAATTACTAACCAAAAACAACTAGAAAGCTCACTTAAACTACAGCGAAACGAATTTGAAGAGAAAATCAACCAGCAAAACTTAACGATTGAAAAACTGAAAATTCAGCAAGCAAGAAGTAGTATCTGA", "aa": "MLNDKEQALLLKTKLELQNQFQKQIENYLNEINKLKLINKELEITNQKQLESSLKLQRNEFEEKINQQNLTIEKLKIQQARSSI", "aa_hexdigest": "2152f6bcbaba56469fb904793e0efb61", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10067.490399999991, "isoelectric_point": 8.925728416442873 }, "id": "JPCNHPFPBM_367", "locus": "GCA000292505_00331" }, { "type": "cds", "sequence": "contig_1", "start": 187834, "stop": 188298, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGTGAGTTTAATGATAGTAAGCATAAATCAAAAAACAAGGACCATATTAGCAAACTGGTCAGAGATGCTAAACGTGCTAACTGCAAGTATGCTTTTTTAATTAGTGAACTTGAATTAGAGACTGAAAATGACATCCAAGTACGCTTAATGCCAACATTGGAAAGTGGTGTTGAAGTCTATCTAGTTAGACCAATGTTTTTTATCTTAATGCTTAAACTTTTCTATAAGTTAGCTAAGAAGTTGTTTGCCCTTAACCGTTTTCAATCAGTTGAACTAATTGATAAAAATAAGTTAAATGAACAGTTTAAACAGTTGAAAGATAATTTTTTAACCAAAACCTTTTTAGAGATTGAAAAAGTGTGTAAAAGTAACTTAGTTGATATTGAAACACTTGAAAAAGCAGTGGTGAAACTAAGGGTTAGAAACGAACGTGTTTTAGATCAGTTACTTAATAAATGA", "aa": "MKSEFNDSKHKSKNKDHISKLVRDAKRANCKYAFLISELELETENDIQVRLMPTLESGVEVYLVRPMFFILMLKLFYKLAKKLFALNRFQSVELIDKNKLNEQFKQLKDNFLTKTFLEIEKVCKSNLVDIETLEKAVVKLRVRNERVLDQLLNK", "aa_hexdigest": "4ef5e11ec56d49368a7dc844cc105c97", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18255.3119, "isoelectric_point": 9.450823402404787 }, "id": "JPCNHPFPBM_368", "locus": "GCA000292505_00332" }, { "type": "cds", "sequence": "contig_1", "start": 188434, "stop": 189144, "strand": "+", "frame": 1, "gene": null, "product": "MPN162 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47395", "UniRef:UniRef90_P47395" ], "nt": "ATGAAATTATACCGATCTTTAAAAGCAGCCCTGTTACCAGGGATATGCACTAGCATTTTACTTGCTAGTTGTGCTTCAACCAATACTTATCAAGACCAAAGGAATGCCTTGATTAGTTTGGCTTCTAACCGTGATACTTTAATTGAAAATGCTAAAAAATCTGAAGAGGAAATAAAAAAAGATGTAATGAAAACTAATGATACTTCATCAATGATGACAGCTACCCAAAGTGTTGCAATTACAACACATCAAACTACTGCTCAAAACAGTTCCAAATATGATTTAGACAAGCTTTTTAAGGATTACATCCTTTATGTTGTTGATAATTTTTCAGGACTTGTTTTTAAAAGAACTGGTGGTCATAGGATCCAGTTAATCGATAAGGATAAAGAGATTTTGGATGGTGGTAATCTAACTAAACATACCCACCATGATCATAACCATATGCATGAACATGAACATGAAGAACACCATGATGAAGAAGAGACAGAAGTAGTTGGCAGAGCACTCTCTTTTACTAATGGCATCTTTCTAGTGATTGACTATAAAAAAGACTCTGAAAGAAAAAATATGAGTGGTTCAACTACTATGATGCAACACCACCATGAAGCTGGTGAACATAAAGAAGAGGAAGAACGTAAGCTCTCTTTAAACTTAAAAGCATACAAATTTAATACTCCTTTTAACATTAGTGAGTTTATTAGTGCTTGA", "aa": "MKLYRSLKAALLPGICTSILLASCASTNTYQDQRNALISLASNRDTLIENAKKSEEEIKKDVMKTNDTSSMMTATQSVAITTHQTTAQNSSKYDLDKLFKDYILYVVDNFSGLVFKRTGGHRIQLIDKDKEILDGGNLTKHTHHDHNHMHEHEHEEHHDEEETEVVGRALSFTNGIFLVIDYKKDSERKNMSGSTTMMQHHHEAGEHKEEEERKLSLNLKAYKFNTPFNISEFISA", "aa_hexdigest": "2f8de1c2162de5dcf93eeb5ae88c74e7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47395", "query_cov": 1.0169491525423728, "subject_cov": 0.8540925266903915, "identity": 0.9, "score": 337.0, "evalue": 2.45e-114, "valid": true, "product": "MPN162 family protein", "uniref50_id": "UniRef50_P47395", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47395", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47395" ], "product": "Uncharacterized lipoprotein MG149" }, "genes": [], "id": "JPCNHPFPBM_369", "locus": "GCA000292505_00333" }, { "type": "cds", "sequence": "contig_1", "start": 189364, "stop": 189456, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAACAAAACCCTGATCGTTTAAAAAAAGATAAGGAACTTATTTATGCAATTGTTACAGCTAAAGGTATCATTAGCCGTTTCTTTTGA", "aa": "MEQQNPDRLKKDKELIYAIVTAKGIISRFF", "aa_hexdigest": "b9ec8c482b09e23c46ef70bc8c2f200e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3553.1366, "isoelectric_point": 9.397443580627442 }, "id": "JPCNHPFPBM_370", "locus": "GCA000292505_00334" }, { "type": "cds", "sequence": "contig_1", "start": 189511, "stop": 189774, "strand": "+", "frame": 1, "gene": null, "product": "MPN163 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_Q9ZB80", "UniRef:UniRef90_Q9ZB80" ], "nt": "GTGCTCTTAATTTATCTGTTAGCAAGTGTTGATAACCAGTTTGCATTTGCTTTTATTGCTGCAATTATCTTCATTATTTTTTACAACATCTTCTTTTTAAGTTACCTGTTGTTTATCTATTTTAAGGGCCAGAAAGCAATTGAAAACAACTGTAAGTACCTGTTAACAATCCTTGATATTAAGAGTGATGAACTGTTACCTTTTTCGCTTTTAGGTAGTTTAAGAAAAGGTTATATGCTAGATGAAATGCTCTTAGAACAGTAA", "aa": "MLLIYLLASVDNQFAFAFIAAIIFIIFYNIFFLSYLLFIYFKGQKAIENNCKYLLTILDIKSDELLPFSLLGSLRKGYMLDEMLLEQ", "aa_hexdigest": "613e0cbc47dfdab43d957c33dbc5b03b", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 189310, "stop": 189774, "observations": { "mutation": [ 189346, 189454 ], "stop": [ 189346, 189454 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9ZB80", "query_cov": 0.9885057471264368, "subject_cov": 0.5584415584415584, "identity": 0.988, "score": 155.0, "evalue": 8.6e-47, "gene_start": 2, "gene_end": 87, "reference_start": 69, "reference_end": 154, "reference_sequence": "MTVYTYTVFLFNWENIGLMEQQNPDRLKKDRELIYAIVTAKGIISRFFWSILSFLITNLIFFFAAFVALLIYLLASVDNQFAFVFIAAIIFIIFYNIFFLSYLLFIYFKGQKAIENNCKYLLTILDIKSDELLPFSLLGSLRKGYMLDEMLLEQ" }, "paralog": false, "identity": 0.961038961038961, "score": 285.0, "evalue": 1.25e-100, "description": "Internal stop codon at 189346, 189454. Nonsense mutation around 189346, 189454.", "psc": { "uniref90_id": "UniRef90_Q9ZB80", "product": "MPN163 family protein", "uniref50_id": "UniRef50_Q9ZB80", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9ZB80", "UniRef:UniRef50_Q9ZB80", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZB80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZB80" ], "product": "Uncharacterized protein MG149.1" } }, "id": "JPCNHPFPBM_371", "locus": "GCA000292505_00335" }, { "type": "cds", "sequence": "contig_1", "start": 189864, "stop": 190184, "strand": "+", "frame": 3, "gene": "rpsJ", "product": "30S ribosomal protein S10", "db_xrefs": [ "COG:COG0051", "COG:J", "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0022627", "RefSeq:WP_009885835.1", "SO:0001217", "UniParc:UPI0000134BB3", "UniRef:UniRef100_P47396", "UniRef:UniRef50_O52331", "UniRef:UniRef90_P75581" ], "nt": "ATGAATAGCGCTGTAAAATATCCTGAGCTGAAGATCAAACTTGAGTCTTATGATAGCACGCTTTTAGATCTCACTATTAAAAAGATAGTTGAGGTTGTAAAGGGTGTGAACATTAAGATTAAAGGTCCTTTACCTTTACCTACTAAAAAGGAAGTGATCACCATTATCCGCTCTCCCCATGTTGATAAAGCATCCAGAGAGCAGTTTGAAAAAAATACCCACAAGCGTTTAATGATCCTTGTTGATGTTAATCAAGGAGGGATTGATAGTTTAAAAAAGATTAAGATCCCAGTTGGGGTTACACTGCGTTTTTCAAAATAG", "aa": "MNSAVKYPELKIKLESYDSTLLDLTIKKIVEVVKGVNIKIKGPLPLPTKKEVITIIRSPHVDKASREQFEKNTHKRLMILVDVNQGGIDSLKKIKIPVGVTLRFSK", "aa_hexdigest": "81c51a3a40e700b1bc8088cf9449d925", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134BB3", "ncbi_nrp_id": "WP_009885835.1", "uniref100_id": "UniRef100_P47396", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134BB3", "RefSeq:WP_009885835.1", "UniRef:UniRef100_P47396" ] }, "ips": { "uniref100_id": "UniRef100_P47396", "uniref90_id": "UniRef90_P75581" }, "psc": { "uniref90_id": "UniRef90_P75581", "gene": "rpsJ", "product": "30S ribosomal protein S10", "uniref50_id": "UniRef50_O52331", "cog_id": "COG0051", "cog_category": "J", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0015935", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_O52331", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52331" ], "product": "Small ribosomal subunit protein uS10" }, "genes": [ "rpsJ" ], "id": "JPCNHPFPBM_372", "locus": "GCA000292505_00336" }, { "type": "cds", "sequence": "contig_1", "start": 190188, "stop": 190565, "strand": "+", "frame": 3, "gene": "rplC", "product": "50S ribosomal protein L3", "db_xrefs": [ "COG:COG0087", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "KEGG:K02906", "SO:0001217", "UniRef:UniRef50_P75580", "UniRef:UniRef90_P47397" ], "nt": "ATGGATGTAAGGGGAATATTTGGTGTTAAAGTAGGGATGAGTCAGATCTTTACTGAACAAAATGAGTGCTTACCTATCACCATTGTTTATTGTGAAGCTAATCAGGTGGCTGGGATTAAAACGATTGCTAAAGATAATTACAACGCCACTCTATTAAGCTTTCAAACTGTTGATGAAAAACAACTTAACAAACCTAAACAAGGGTTCTTTTCCAAACTTAAACTAGCACCTCATAAATATCTGAGGGAAATCAGAAAGATGCAAGGGTTTGAGTTAGGTAAGAAGATCACCCCCCAGGAGTTGTTTAAGATAGGTGAATATGTTGATGTCACTTCACTCACCAAAGGTAGGGGTTTTACAGGAGCGATTAAAAGGTGA", "aa": "MDVRGIFGVKVGMSQIFTEQNECLPITIVYCEANQVAGIKTIAKDNYNATLLSFQTVDEKQLNKPKQGFFSKLKLAPHKYLREIRKMQGFELGKKITPQELFKIGEYVDVTSLTKGRGFTGAIKR", "aa_hexdigest": "814645e05c8bf10345a700931d7319dc", "start_type": "ATG", "rbs_motif": null, "genes": [ "rplC" ], "truncated": "3-prime", "pseudogene": { "start": 190188, "stop": 190868, "observations": { "mutation": [ 190563 ], "stop": [ 190563 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47397", "query_cov": 1.0, "subject_cov": 0.48638132295719844, "identity": 0.992, "score": 249.0, "evalue": 5.99e-82, "gene_start": 1, "gene_end": 125, "reference_start": 1, "reference_end": 125, "reference_sequence": "MDVRGIFGVKVGMSQIFTEQNECLPITIVYCEANQVAGIKTIAKDNYNATLLSFQTVDEKQLNKPKQGFFSKLKLEPHKYLREIRKMQGFELGKKITPQELFKIGEYVDVTSLTKGRGFTGAIKRWNFKIGPLGHGAGYPHRFQGSVQAGRGGSSAQRVFKGKKMSGHYGHEQVTIQNLFIVGFDEINKLVLVSGAIAGPEGGIVLIKTAKKKTGKIKDIKLAVQTVKAPQLKAPKKQKTKVETNQVNPKIEEEKTK" }, "paralog": false, "identity": 0.9867256637168141, "score": 445.0, "evalue": 1.45e-161, "description": "Internal stop codon at 190563. Nonsense mutation around 190563.", "psc": { "uniref90_id": "UniRef90_P47397", "gene": "rplC", "product": "50S ribosomal protein L3", "uniref50_id": "UniRef50_P75580", "cog_id": "COG0087", "cog_category": "J", "kegg_orthology_id": "K02906", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47397", "UniRef:UniRef50_P75580", "COG:COG0087", "COG:J", "KEGG:K02906", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P75580", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75580" ], "product": "Large ribosomal subunit protein uL3" } }, "id": "JPCNHPFPBM_373", "locus": "GCA000292505_00337" }, { "type": "cds", "sequence": "contig_1", "start": 190584, "stop": 190961, "strand": "+", "frame": 3, "gene": "rplC", "product": "50S ribosomal protein L3", "db_xrefs": [ "COG:COG0087", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "KEGG:K02906", "SO:0001217", "UniRef:UniRef50_P75580", "UniRef:UniRef90_P47397" ], "nt": "TTGGGCCATGGGGCGGGTTATCCCCACCGCTTTCAGGGTTCAGTGCAAGCAGGTAGAGGTGGTAGTAGTGCGCAGCGTGTTTTTAAGGGTAAGAAGATGTCTGGGCATTATGGTCATGAACAAGTTACGATCCAAAATCTCTTTATTGTTGGCTTTGATGAAATCAATAAGTTAGTGTTAGTTTCAGGCGCAATTGCTGGTCCTGAGGGTGGGATTGTTTTAATTAAAACTGCAAGAAAGAAAACTGGCAAGATAAAAGATATAAAGTTAGCAGTACAAACTGTTAAAGCCCCACAACTAAAAGCACCAAAAAAGCAGAAAACTAAGGTTGAAACCAACCAGGTTAACCCAAAAATTGAAGAAGAGAAAACTAAGTAA", "aa": "MGHGAGYPHRFQGSVQAGRGGSSAQRVFKGKKMSGHYGHEQVTIQNLFIVGFDEINKLVLVSGAIAGPEGGIVLIKTARKKTGKIKDIKLAVQTVKAPQLKAPKKQKTKVETNQVNPKIEEEKTK", "aa_hexdigest": "def43abacf93a1a9dc76e9c31c79fcfb", "start_type": "TTG", "rbs_motif": null, "genes": [ "rplC" ], "truncated": "5-prime", "pseudogene": { "start": 190284, "stop": 190961, "observations": { "mutation": [ 190563 ], "stop": [ 190563 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47397", "query_cov": 1.0, "subject_cov": 0.48638132295719844, "identity": 0.9840000000000001, "score": 233.0, "evalue": 2.05e-75, "gene_start": 1, "gene_end": 125, "reference_start": 133, "reference_end": 257, "reference_sequence": "MDVRGIFGVKVGMSQIFTEQNECLPITIVYCEANQVAGIKTIAKDNYNATLLSFQTVDEKQLNKPKQGFFSKLKLEPHKYLREIRKMQGFELGKKITPQELFKIGEYVDVTSLTKGRGFTGAIKRWNFKIGPLGHGAGYPHRFQGSVQAGRGGSSAQRVFKGKKMSGHYGHEQVTIQNLFIVGFDEINKLVLVSGAIAGPEGGIVLIKTAKKKTGKIKDIKLAVQTVKAPQLKAPKKQKTKVETNQVNPKIEEEKTK" }, "paralog": false, "identity": 0.9866666666666667, "score": 440.0, "evalue": 1.38e-159, "description": "Internal stop codon at 190563. Nonsense mutation around 190563.", "psc": { "uniref90_id": "UniRef90_P47397", "gene": "rplC", "product": "50S ribosomal protein L3", "uniref50_id": "UniRef50_P75580", "cog_id": "COG0087", "cog_category": "J", "kegg_orthology_id": "K02906", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47397", "UniRef:UniRef50_P75580", "COG:COG0087", "COG:J", "KEGG:K02906", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P75580", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75580" ], "product": "Large ribosomal subunit protein uL3" } }, "id": "JPCNHPFPBM_374", "locus": "GCA000292505_00338" }, { "type": "cds", "sequence": "contig_1", "start": 190961, "stop": 191596, "strand": "+", "frame": 2, "gene": "rplD", "product": "50S ribosomal protein L4", "db_xrefs": [ "COG:COG0088", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:1990904", "RefSeq:WP_014894598.1", "SO:0001217", "UniParc:UPI00027B366A", "UniRef:UniRef100_UPI00027B366A", "UniRef:UniRef50_P75579", "UniRef:UniRef90_P47398" ], "nt": "ATGGCTAAGCTTAAAGTAATCCAGTTTGATGGTAGTTTTAAAGGTGAGATCCAACCTGCTAACCACCTCCTTTTAAAAAAAGCAGTGATCCAACCAGTGTTTGATGCTATCTTATTAGAACAAGCAGCATGTAGACAAGGCACTCACTCCACTTTAACTAAGGGTGAAGTTAGTGGTGGGGGTAAAAAACCATATAAACAAAAGCACACTGGTAAAGCTAGACAAGGTTCAATAAGAAACCCCCATTATGTGGGGGGAGGTGTTGTTTTTGGTCCTAAACCCAACCGTAACTACAAACTAAAACTAAACAAAAAGGCTTATCAACTTGCTTTAACTAGTGTATTTGTACAAAAGCTTAATAACAACCAAGTGATAGTTGCTGAAGCCAAGTTGTTTGAACAAACCAATGCCAAAACTAAAAAGATGCTAACGTTTCTTAAGAATGCCAAATTAACTGAGCAAAAACTCTTGTTTGTGATTGATACTATCTCAAAACCACTGTTGTTAAGTACTAGTAACCTAAAGCAGATAGTAGTCAAACAGTTTAATAAAGTATCAGTAAGAGATCTACTTTTAGCTAAAACTATCATCATTGAAAAAGCTGCTTTTACAAAACTGGAGGAACGACTTAAATAG", "aa": "MAKLKVIQFDGSFKGEIQPANHLLLKKAVIQPVFDAILLEQAACRQGTHSTLTKGEVSGGGKKPYKQKHTGKARQGSIRNPHYVGGGVVFGPKPNRNYKLKLNKKAYQLALTSVFVQKLNNNQVIVAEAKLFEQTNAKTKKMLTFLKNAKLTEQKLLFVIDTISKPLLLSTSNLKQIVVKQFNKVSVRDLLLAKTIIIEKAAFTKLEERLK", "aa_hexdigest": "4388c9e1882a906af03e355161899a5c", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B366A", "ncbi_nrp_id": "WP_014894598.1", "uniref100_id": "UniRef100_UPI00027B366A", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B366A", "RefSeq:WP_014894598.1", "UniRef:UniRef100_UPI00027B366A" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B366A", "uniref90_id": "UniRef90_P47398" }, "psc": { "uniref90_id": "UniRef90_P47398", "gene": "rplD", "product": "50S ribosomal protein L4", "uniref50_id": "UniRef50_P75579", "cog_id": "COG0088", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P75579", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75579" ], "product": "Large ribosomal subunit protein uL4" }, "genes": [ "rplD" ], "id": "JPCNHPFPBM_375", "locus": "GCA000292505_00339" }, { "type": "cds", "sequence": "contig_1", "start": 191600, "stop": 191920, "strand": "+", "frame": 2, "gene": "rplW", "product": "50S ribosomal protein L23", "db_xrefs": [ "COG:COG0089", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "RefSeq:WP_009885837.1", "SO:0001217", "UniParc:UPI0000133DA2", "UniRef:UniRef100_P47399", "UniRef:UniRef50_P47399", "UniRef:UniRef90_P47399" ], "nt": "ATGGATGTAACCAACATACTCTTAAAACCAGTCTTAACTGAAAAGAGTTATCTCAACCAGATGGGGGAATTGAAAAAATATGTCTTTGCAATTAACCCTAAAGCTACTAAAACTAAAGTAAAACTAGCGTTTGAAATTATCTATGGGGTTAAACCTTTAAAGATTAACACGCTAATTAGAAAGCCAGTGACCATTAGAAATGGCACTAAATACCCTGGGTTTAGTAAGCTAGCAAAACTAGCAGTAATCACCTTACCTAAGGGAATGGATATTGCCATTACTGGTGAGAAAACAACTAAGAAAGAAACAAAGGATCAATAA", "aa": "MDVTNILLKPVLTEKSYLNQMGELKKYVFAINPKATKTKVKLAFEIIYGVKPLKINTLIRKPVTIRNGTKYPGFSKLAKLAVITLPKGMDIAITGEKTTKKETKDQ", "aa_hexdigest": "cb3bde973f635e2c137d7725dd7ff6fe", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133DA2", "ncbi_nrp_id": "WP_009885837.1", "uniref100_id": "UniRef100_P47399", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133DA2", "RefSeq:WP_009885837.1", "UniRef:UniRef100_P47399" ] }, "ips": { "uniref100_id": "UniRef100_P47399", "uniref90_id": "UniRef90_P47399" }, "psc": { "uniref90_id": "UniRef90_P47399", "gene": "rplW", "product": "50S ribosomal protein L23", "uniref50_id": "UniRef50_P47399", "cog_id": "COG0089", "cog_category": "J", "go_ids": [ "GO:1990904", "GO:0005840", "GO:0019843", "GO:0003735", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P47399", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47399" ], "product": "Large ribosomal subunit protein uL23" }, "genes": [ "rplW" ], "id": "JPCNHPFPBM_376", "locus": "GCA000292505_00340" }, { "type": "cds", "sequence": "contig_1", "start": 191920, "stop": 192777, "strand": "+", "frame": 1, "gene": "rplB", "product": "50S ribosomal protein L2", "db_xrefs": [ "COG:COG0090", "COG:J", "GO:0002181", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740", "GO:0019843", "GO:0022625", "KEGG:K02886", "RefSeq:WP_014894599.1", "SO:0001217", "UniParc:UPI00027B366B", "UniRef:UniRef100_UPI00027B366B", "UniRef:UniRef50_Q9AMK8", "UniRef:UniRef90_P47400" ], "nt": "ATGGCAATTAAAAAGATTATTAGTCGTTCTAACAGTGGGATTCACAACACCACTGTCATTGACTTTAAAAAACTCCTTACTAATTCCAAACCTGAAAAGTCGCTTTTAGTTACTTTAAAAAAACATGCAGGAAGAAACAACCAAGGCAAGATCACTGTTCGCCACCACGGTGGGAGACATAAACGTAAGTACCGTTTAATTGATTTTAAGCGTTACCACTATGACAATTTAAAAGCAACTGTTAAATCGATTGAATATGATCCTAACCGCAGTTGTTTTATCTCCCTTTTACACTATCAGAATGGGGTTAAAACTTACATCATTAGTCCTGATGGGATTAAGGTTGGTGATCAAGTTTATTCATCTGATCATGCCATTGATATCAAACTGGGTTATTGTATGCCCCTTGCTTTTATCCCTGAAGGAACGCAAGTTCATAACATTGAACTTAACCCTAAGGGTGGGGGTAAGATAGCAAGAAGTGCTGGAAGTTATGCGAGGATCTTGGGTCAAGATGAGACTGGTAAATACATCATTCTCCAGTTAATCTCAGGGGAAACTAGGAAGTTTTTAAAGGAGTGTAGAGCTACAGTTGGTGTTGTCTCTAACTTAGATCATAACCTTGTTGTAATTGGTAAAGCAGGGAGAAGTCGTCATAAGGGAATCAGACCAACGGTTAGAGGTTCAGCAATGAACCCTAATGACCACCCGCATGGGGGTGGGGAAGGGAGAAGCCCAGTTGGCAGAGATGCACCAAGAACCCCTTGGGGCAAACGCCATATGGGTGTGAAAACACGTAACATGAAAAAACATTCAACTAACCTGATTATTAGAAACAGAAAAGGAGAACAATACTAA", "aa": "MAIKKIISRSNSGIHNTTVIDFKKLLTNSKPEKSLLVTLKKHAGRNNQGKITVRHHGGRHKRKYRLIDFKRYHYDNLKATVKSIEYDPNRSCFISLLHYQNGVKTYIISPDGIKVGDQVYSSDHAIDIKLGYCMPLAFIPEGTQVHNIELNPKGGGKIARSAGSYARILGQDETGKYIILQLISGETRKFLKECRATVGVVSNLDHNLVVIGKAGRSRHKGIRPTVRGSAMNPNDHPHGGGEGRSPVGRDAPRTPWGKRHMGVKTRNMKKHSTNLIIRNRKGEQY", "aa_hexdigest": "db159daeb80ce51bfe8284e699cf27a5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B366B", "ncbi_nrp_id": "WP_014894599.1", "uniref100_id": "UniRef100_UPI00027B366B", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B366B", "RefSeq:WP_014894599.1", "UniRef:UniRef100_UPI00027B366B" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B366B", "uniref90_id": "UniRef90_P47400" }, "psc": { "uniref90_id": "UniRef90_P47400", "gene": "rplB", "product": "50S ribosomal protein L2", "uniref50_id": "UniRef50_Q9AMK8", "cog_id": "COG0090", "cog_category": "J", "kegg_orthology_id": "K02886", "go_ids": [ "GO:0002181", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q9AMK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9AMK8" ], "product": "Large ribosomal subunit protein uL2" }, "genes": [ "rplB" ], "id": "JPCNHPFPBM_377", "locus": "GCA000292505_00341" }, { "type": "cds", "sequence": "contig_1", "start": 192777, "stop": 193040, "strand": "+", "frame": 3, "gene": "rpsS", "product": "30S ribosomal protein S19", "db_xrefs": [ "COG:COG0185", "COG:J", "GO:0000028", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0022627", "RefSeq:WP_009885839.1", "SO:0001217", "UniParc:UPI0000134DEA", "UniRef:UniRef100_P47401", "UniRef:UniRef50_Q6ME59", "UniRef:UniRef90_P75576" ], "nt": "ATGTCAAGAAGTAGTAAAAAGGGCGCATTTGTTGATGCTCACCTCTTAAAAAAAGTGATTGAAATGAACAAACAAGCCAAGAAAAAACCAATTAAGACTTGGTCAAGAAGAAGTACTATCTTCCCTGAGTTTGTGGGTAACACCTTCAGTGTGCATAACGGTAAAACCTTTATTAATGTTTATGTTACTGATGATATGGTAGGTCATAAGTTGGGTGAGTTTTCCCCAACTAGAAACTTTAAACAACACACTGCTAACCGTTAG", "aa": "MSRSSKKGAFVDAHLLKKVIEMNKQAKKKPIKTWSRRSTIFPEFVGNTFSVHNGKTFINVYVTDDMVGHKLGEFSPTRNFKQHTANR", "aa_hexdigest": "3f214f53aa7f11b4874ded3835d887c3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134DEA", "ncbi_nrp_id": "WP_009885839.1", "uniref100_id": "UniRef100_P47401", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134DEA", "RefSeq:WP_009885839.1", "UniRef:UniRef100_P47401" ] }, "ips": { "uniref100_id": "UniRef100_P47401", "uniref90_id": "UniRef90_P75576" }, "psc": { "uniref90_id": "UniRef90_P75576", "gene": "rpsS", "product": "30S ribosomal protein S19", "uniref50_id": "UniRef50_Q6ME59", "cog_id": "COG0185", "cog_category": "J", "go_ids": [ "GO:0000028", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_Q6ME59", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6ME59" ], "product": "Small ribosomal subunit protein uS19" }, "genes": [ "rpsS" ], "id": "JPCNHPFPBM_378", "locus": "GCA000292505_00342" }, { "type": "cds", "sequence": "contig_1", "start": 193043, "stop": 193477, "strand": "+", "frame": 2, "gene": "rplV", "product": "50S ribosomal protein L22", "db_xrefs": [ "COG:COG0091", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625", "KEGG:K02890", "RefSeq:WP_009885840.1", "SO:0001217", "UniParc:UPI0000133D78", "UniRef:UniRef100_P47402", "UniRef:UniRef50_P47402", "UniRef:UniRef90_P47402" ], "nt": "ATGATTGCTTTTGCTAAACAATACAGAGTTCACATCTCCCCCCAAAAAGCACGGTTAGTGTGCCAGTTAATTGTGGGTAAGAAGATTAATGATGCGCAAAACATCCTTTTAAATACGCCAAAGAAAGCTGCTTACTTTTTAACTAAGTTACTAAATAGTGCGATTAGTAATGCCACTAATAACCACGGGATGAGCGGGGATCTTTTGTATGTATTTGAATGTGTTGCTAACCAAGGACCTAGCATGAAAAGAACAATCGCTAGAGCCAAAGGTTCAGGGAGTGTTTTAACCAAGCGTTCTTCAAACCTAGTTATTAAGTTATCTGATAATCCCAATGAAAGAAAATTACTTTTAACCCAACAAAAGGAACTGGTGAAAAAAAGAACAATGGGTCATAAAAAAGAGAAAGCAAAGCAAAAGCAAAAACAACAATAA", "aa": "MIAFAKQYRVHISPQKARLVCQLIVGKKINDAQNILLNTPKKAAYFLTKLLNSAISNATNNHGMSGDLLYVFECVANQGPSMKRTIARAKGSGSVLTKRSSNLVIKLSDNPNERKLLLTQQKELVKKRTMGHKKEKAKQKQKQQ", "aa_hexdigest": "5ef95273909311097b2a84d0940b84a8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133D78", "ncbi_nrp_id": "WP_009885840.1", "uniref100_id": "UniRef100_P47402", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133D78", "RefSeq:WP_009885840.1", "UniRef:UniRef100_P47402" ] }, "ips": { "uniref100_id": "UniRef100_P47402", "uniref90_id": "UniRef90_P47402" }, "psc": { "uniref90_id": "UniRef90_P47402", "gene": "rplV", "product": "50S ribosomal protein L22", "uniref50_id": "UniRef50_P47402", "cog_id": "COG0091", "cog_category": "J", "kegg_orthology_id": "K02890", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47402", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47402" ], "product": "Large ribosomal subunit protein uL22" }, "genes": [ "rplV" ], "id": "JPCNHPFPBM_379", "locus": "GCA000292505_00343" }, { "type": "cds", "sequence": "contig_1", "start": 193636, "stop": 194286, "strand": "+", "frame": 1, "gene": "rpsC", "product": "30S ribosomal protein S3", "db_xrefs": [ "COG:COG0092", "COG:J", "GO:0003729", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022627", "KEGG:K02982", "SO:0001217", "UniRef:UniRef50_O52338", "UniRef:UniRef90_P47403" ], "nt": "GTGTCTAATGTTGAGATAGAAAGAACCCAAACTACTGTTGATGTTTATGTCTATGCAGCTCAACCTGCTTTATTGATAGGCAGTGAAAATAAAAACATCCAAAAGATTACCAAAATGATCCAAATCATTGTGGGCAGAAAGATTAAACTTGATCTTACTATCAATGAGATCGGCTCTCCGATGCTATCAAGTAGGATCATTGCCCGTGATATTGCTAATGCAATTGAAAACAGAGTACCACTCCGTTCAGCAATGCGCCAAGCTCTAACCAAGGTTTTAAAAGCAGGTGCTAATGGGATTAAGGTATTGGTATCAGGCAGATTAAATGGGGCGGAAATTGCCCGTGACAAGATGTATATTGAGGGCAATATGCCTCTTTCAACTTTAAGAGCAGATATTGACTATGCCTTTGAAAAAGCAAAAACCACCTATGGCATTATTGGGGTGAAAGTATGGATTAACAGGGGGATGATCTATGCAAAGGGTTTAAACAGAACCCCAGCACACATCCTCCATCCCCAAAAGAAACAGCTAAAAACCCCAACTATCAAAAAAACCAATTCAGTAATAGCAAAACAAAAACTCACTGGTAGTGATATTGAAACTGCTAGTTTAAAAGCACTTACTGATAATAATCAAAACCACGAATAG", "aa": "MSNVEIERTQTTVDVYVYAAQPALLIGSENKNIQKITKMIQIIVGRKIKLDLTINEIGSPMLSSRIIARDIANAIENRVPLRSAMRQALTKVLKAGANGIKVLVSGRLNGAEIARDKMYIEGNMPLSTLRADIDYAFEKAKTTYGIIGVKVWINRGMIYAKGLNRTPAHILHPQKKQLKTPTIKKTNSVIAKQKLTGSDIETASLKALTDNNQNHE", "aa_hexdigest": "44fb446fc3d65b5806df197fd2afc470", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47403", "query_cov": 1.0, "subject_cov": 0.8059701492537313, "identity": 0.995, "score": 367.0, "evalue": 1.36e-126, "valid": true, "gene": "rpsC", "product": "30S ribosomal protein S3", "uniref50_id": "UniRef50_O52338", "cog_id": "COG0092", "cog_category": "J", "kegg_orthology_id": "K02982", "go_ids": [ "GO:0003729", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_O52338", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52338" ], "product": "Small ribosomal subunit protein uS3" }, "genes": [ "rpsC" ], "id": "JPCNHPFPBM_380", "locus": "GCA000292505_00344" }, { "type": "cds", "sequence": "contig_1", "start": 194292, "stop": 194486, "strand": "+", "frame": 3, "gene": "rplP", "product": "50S ribosomal protein L16", "db_xrefs": [ "COG:COG0197", "COG:J", "GO:0000049", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022625", "KEGG:K02878", "SO:0001217", "UniRef:UniRef50_P02415", "UniRef:UniRef90_P47404" ], "nt": "ATGTTACAACCAAAAAGAACCAAATACAGAAAACCACATAACGTCAGTTATGAAGGACACACTAAGGGCAATGGTTATGTTGCTTTTGGTGAGTATGGAATTGTTGCTACTAAGGGTAATTGGATCGATGCGAGAGCAATTGAATCAGCGCGGGTTGCTATCTCAAAGTGCTTGGGTAAAACTGGAAAGATGTGA", "aa": "MLQPKRTKYRKPHNVSYEGHTKGNGYVAFGEYGIVATKGNWIDARAIESARVAISKCLGKTGKM", "aa_hexdigest": "b9d8b1d6fece06001424e9d7004b3a9a", "start_type": "ATG", "rbs_motif": null, "genes": [ "rplP" ], "truncated": "3-prime", "pseudogene": { "start": 194292, "stop": 194708, "observations": { "mutation": [ 194484, 194676 ], "stop": [ 194484, 194676 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47404", "query_cov": 1.0, "subject_cov": 0.460431654676259, "identity": 0.922, "score": 124.0, "evalue": 4.4e-35, "gene_start": 1, "gene_end": 64, "reference_start": 1, "reference_end": 64, "reference_sequence": "MLQPKRTKYRKPHNVSYEGKAKGNSYVAFGEYGLVATKGNWIDARAIESARIAISKCLGKTGKMWIRIFPHMSKTKKPLEVRMGSGKGNPEFWVAVVKQGTVMFEVANIPESQMIKALTRAGHKLPVTWKILKREEVSA" }, "paralog": false, "identity": 0.9130434782608695, "score": 258.0, "evalue": 1.41e-90, "description": "Internal stop codon at 194484, 194676. Nonsense mutation around 194484, 194676.", "psc": { "uniref90_id": "UniRef90_P47404", "gene": "rplP", "product": "50S ribosomal protein L16", "uniref50_id": "UniRef50_P02415", "cog_id": "COG0197", "cog_category": "J", "kegg_orthology_id": "K02878", "go_ids": [ "GO:0000049", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022625" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47404", "UniRef:UniRef50_P02415", "COG:COG0197", "COG:J", "KEGG:K02878", "GO:0000049", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P02415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P02415" ], "product": "Large ribosomal subunit protein uL16" } }, "id": "JPCNHPFPBM_381", "locus": "GCA000292505_00345" }, { "type": "cds", "sequence": "contig_1", "start": 194505, "stop": 194678, "strand": "+", "frame": 3, "gene": null, "product": "Large ribosomal subunit protein uL16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_M5SUM2" ], "nt": "ATGTCAAAAACCAAAAAACCCTTAGAAGTGAGGATGGGTTCAGGGAAAGGTAACCCTGAATTTTGGGTTGCTGTTGTTAAGAAGGGGACAGTGATGTTTGAAGTTGCTAACATCCCTGAACAACAGATGATCAAAGCCTTAACAAGAGCAGGCCATAAACTCCCTGTTACCTGA", "aa": "MSKTKKPLEVRMGSGKGNPEFWVAVVKKGTVMFEVANIPEQQMIKALTRAGHKLPVT", "aa_hexdigest": "40ea8c93f2f0ea231b9d0e2882bcb9b2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_M5SUM2", "query_cov": 0.9298245614035088, "subject_cov": 0.828125, "identity": 0.509, "score": 63.9, "evalue": 2.35e-12, "valid": false, "product": "Large ribosomal subunit protein uL16", "uniref50_id": "UniRef50_M5SUM2" }, "pscc": { "uniref50_id": "UniRef50_M5SUM2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_M5SUM2" ], "product": "Large ribosomal subunit protein uL16" }, "genes": [], "id": "JPCNHPFPBM_382", "locus": "GCA000292505_00346" }, { "type": "cds", "sequence": "contig_1", "start": 194708, "stop": 194902, "strand": "+", "frame": 2, "gene": "rpmC", "product": "50S ribosomal protein L29", "db_xrefs": [ "GO:0003735", "GO:0005840", "GO:0006412", "SO:0001217", "UniRef:UniRef50_UPI0029620CC7", "UniRef:UniRef90_UPI0029620CC7" ], "nt": "ATGACAATCGCTAAGGAGCTGAAGCAAAAGAGCAACGAAGAGTTAGTGAAACTAGTAATTAAGCTTAAGGGTGAACTCTTAGAATACCGCTTTAAACTTGCCCATGGTGAACTTGACAAACCCCATCTGATTGCCAAGGTAAGAAAGTTATTAGCAGTTGTACTTACTATTCTCACTGAACGCAAAATCAACTGA", "aa": "MTIAKELKQKSNEELVKLVIKLKGELLEYRFKLAHGELDKPHLIAKVRKLLAVVLTILTERKIN", "aa_hexdigest": "a1a0a118a73255a4ccbcbe00aba38d9d", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpmC" ], "truncated": "3-prime", "pseudogene": { "start": 194714, "stop": 195058, "observations": { "mutation": [ 194900, 194966 ], "stop": [ 194900, 194966 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0029620CC7", "query_cov": 0.96875, "subject_cov": 0.4460431654676259, "identity": 0.9840000000000001, "score": 112.0, "evalue": 1.6e-30, "gene_start": 3, "gene_end": 64, "reference_start": 1, "reference_end": 62, "reference_sequence": "MAKELKQKSNEELVKLVIKLKGELLEYRFKLAHGELDKPHLIAKVRKLLAVVLTILTERKINWQVEKDKYKLLSRKTNELIVNSWKQKLSTKPESKQETKKAEVKPKVEPKPLKQETKKVEENKKLKRLKLNLKLNQNL" }, "paralog": false, "identity": 0.9035087719298246, "score": 191.0, "evalue": 3.56e-64, "description": "Internal stop codon at 194900, 194966. Nonsense mutation around 194900, 194966.", "psc": { "uniref90_id": "UniRef90_UPI0029620CC7", "gene": "rpmC", "product": "50S ribosomal protein L29", "uniref50_id": "UniRef50_UPI0029620CC7", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0029620CC7", "UniRef:UniRef50_UPI0029620CC7", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0029620CC7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0029620CC7" ], "product": "50S ribosomal protein L29" } }, "id": "JPCNHPFPBM_383", "locus": "GCA000292505_00347" }, { "type": "cds", "sequence": "contig_1", "start": 195297, "stop": 195521, "strand": "+", "frame": 3, "gene": "rpsQ", "product": "30S ribosomal protein S17", "db_xrefs": [ "COG:COG0186", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "SO:0001217", "UniRef:UniRef50_P47406", "UniRef:UniRef90_P47406" ], "nt": "ATGAAGCGCAACCAACGTAAGCAGTTAATTGGCACAGTTGTTAGCACCAAAAATGCTAAAACAGCAACTGTAAAAGTAACATCACGCTTTAAACATCCTTTGTATCACAAATCAGTTATTCGTCATAAAAAGTACCATGTCCATAACTTTGGTGAACTTGTTGCTAATGATGGTGATAGGGTACAAATTATTGAAACAAGACCCCTTTCCGCTTTAAAGCGGTGA", "aa": "MKRNQRKQLIGTVVSTKNAKTATVKVTSRFKHPLYHKSVIRHKKYHVHNFGELVANDGDRVQIIETRPLSALKR", "aa_hexdigest": "0ccbd0b07373a87d1ad21f01e9db1014", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47406", "query_cov": 1.0, "subject_cov": 0.8705882352941177, "identity": 1.0, "score": 144.0, "evalue": 7.83e-44, "valid": true, "gene": "rpsQ", "product": "30S ribosomal protein S17", "uniref50_id": "UniRef50_P47406", "cog_id": "COG0186", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P47406", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47406" ], "product": "Small ribosomal subunit protein uS17" }, "genes": [ "rpsQ" ], "id": "JPCNHPFPBM_384", "locus": "GCA000292505_00348" }, { "type": "cds", "sequence": "contig_1", "start": 195558, "stop": 195926, "strand": "+", "frame": 3, "gene": "rplN", "product": "50S ribosomal protein L14", "db_xrefs": [ "COG:COG0093", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0022625", "GO:0070180", "KEGG:K02874", "RefSeq:WP_009885845.1", "SO:0001217", "UniParc:UPI0000133BE7", "UniRef:UniRef100_P47407", "UniRef:UniRef50_Q5SHP8", "UniRef:UniRef90_P47407" ], "nt": "ATGGTTAGTTTTATGACAAGATTAAATGTAGCTGATAATACAGGCGCTAAGCAAGTAGGTATTATCAAAGTTTTAGGTGCTACATACAAACGTTATGCATTCCTTGGTGATGTTGTTGTTGTATCAGTTAAAGATGCAATCCCTAATGGCATGGTTAAAAAGGGTCAAGTGTTAAGAGCAGTCATTGTTAGAACCAAAAAGGGACAACAACGCCAAGATGGTACCCACCTAAAGTTCCATGACAATGCTTGTGTGCTTATCAAAGAAGATAAATCCCCAAGGGGAACAAGAATCTTTGGACCAGTTGCTAGAGAGTTGAGAGAAAAAGGTTACAACAAGATTTTAAGCTTGGCGGTGGAGGTTGTTTAA", "aa": "MVSFMTRLNVADNTGAKQVGIIKVLGATYKRYAFLGDVVVVSVKDAIPNGMVKKGQVLRAVIVRTKKGQQRQDGTHLKFHDNACVLIKEDKSPRGTRIFGPVARELREKGYNKILSLAVEVV", "aa_hexdigest": "a3dba71f87df18733f7f3013a4c99584", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133BE7", "ncbi_nrp_id": "WP_009885845.1", "uniref100_id": "UniRef100_P47407", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133BE7", "RefSeq:WP_009885845.1", "UniRef:UniRef100_P47407" ] }, "ips": { "uniref100_id": "UniRef100_P47407", "uniref90_id": "UniRef90_P47407" }, "psc": { "uniref90_id": "UniRef90_P47407", "gene": "rplN", "product": "50S ribosomal protein L14", "uniref50_id": "UniRef50_Q5SHP8", "cog_id": "COG0093", "cog_category": "J", "kegg_orthology_id": "K02874", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0022625", "GO:0070180" ] }, "pscc": { "uniref50_id": "UniRef50_Q5SHP8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5SHP8" ], "product": "Large ribosomal subunit protein uL14" }, "genes": [ "rplN" ], "id": "JPCNHPFPBM_385", "locus": "GCA000292505_00349" }, { "type": "cds", "sequence": "contig_1", "start": 195926, "stop": 196252, "strand": "+", "frame": 2, "gene": "rplX", "product": "50S ribosomal protein L24", "db_xrefs": [ "COG:COG0198", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "RefSeq:WP_009885846.1", "SO:0001217", "UniParc:UPI0000133DCB", "UniRef:UniRef100_P47408", "UniRef:UniRef50_O52343", "UniRef:UniRef90_P47408" ], "nt": "ATGCAAAGGATTAGAAAAGGTGATAAGGTAGTTGTGATCACTGGTAAAAACAAGGGTGGTAGTGGGATAGTGCTTAAGGTATTAACCAAGCAAAACAAAGCGATTGTTGAGGGGATCAATAAGGTTACTGTTCACAAAAAAGAACAAGTCAACAAGCGCAGCAAACAAACAAACCCAACTACTAAAGAAGCCCCTTTACCATTAAATAAACTTGCTTTATTTGATCAGAAGGCCAAACAGCAAACAATTGGCAAGATCAAATACCAAATTGATCCTAAAACCAAACAAAAAACAAGAGTCTTTAAGAAGACTAATAATGCCATTTAA", "aa": "MQRIRKGDKVVVITGKNKGGSGIVLKVLTKQNKAIVEGINKVTVHKKEQVNKRSKQTNPTTKEAPLPLNKLALFDQKAKQQTIGKIKYQIDPKTKQKTRVFKKTNNAI", "aa_hexdigest": "9735561e3d016a8dbea25d505dd52901", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133DCB", "ncbi_nrp_id": "WP_009885846.1", "uniref100_id": "UniRef100_P47408", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133DCB", "RefSeq:WP_009885846.1", "UniRef:UniRef100_P47408" ] }, "ips": { "uniref100_id": "UniRef100_P47408", "uniref90_id": "UniRef90_P47408" }, "psc": { "uniref90_id": "UniRef90_P47408", "gene": "rplX", "product": "50S ribosomal protein L24", "uniref50_id": "UniRef50_O52343", "cog_id": "COG0198", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_O52343", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52343" ], "product": "Large ribosomal subunit protein uL24" }, "genes": [ "rplX" ], "id": "JPCNHPFPBM_386", "locus": "GCA000292505_00350" }, { "type": "cds", "sequence": "contig_1", "start": 196258, "stop": 196548, "strand": "+", "frame": 1, "gene": null, "product": "50S ribosomal protein L5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380ENH6" ], "nt": "ATGAATAACCTTGAAAAAACCTATAAAACTGAGTTAGTTAATCAACTCCAACAACAGTTGGGCTTTTCTTCCATTATGCAAGTCCCTAAGTTAACAAAAATCGTTGTTAACATGGGAGTTGGGGATGCAATTAGAGACAACAAGTTCCTTGAATCAGCACTAAATGAACTGCACCTGATTACTGGTCAAAAACCCGTTGCTACTAAAGCTAAGAATGCTATCTCAACTTACAAGTTACGTGCTGGCCAATTAATTGGTTGTAAAGTTACTCTAAGAAATAAAAAGATGTGA", "aa": "MNNLEKTYKTELVNQLQQQLGFSSIMQVPKLTKIVVNMGVGDAIRDNKFLESALNELHLITGQKPVATKAKNAISTYKLRAGQLIGCKVTLRNKKM", "aa_hexdigest": "041c65435d155804689965cccf73494f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A380ENH6", "query_cov": 0.9791666666666666, "subject_cov": 0.9494949494949495, "identity": 0.5429999999999999, "score": 108.0, "evalue": 6.19e-29, "valid": false, "product": "50S ribosomal protein L5", "uniref50_id": "UniRef50_A0A380ENH6", "kegg_orthology_id": "K02931" }, "pscc": { "uniref50_id": "UniRef50_A0A380ENH6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380ENH6" ], "product": "50S ribosomal protein L5" }, "genes": [], "id": "JPCNHPFPBM_387", "locus": "GCA000292505_00351" }, { "type": "cds", "sequence": "contig_1", "start": 196803, "stop": 196985, "strand": "+", "frame": 3, "gene": "rpsZ", "product": "type Z 30S ribosomal protein S14", "db_xrefs": [ "COG:COG0199", "COG:J", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0008270", "GO:0015935", "GO:0019843", "KEGG:K02954", "SO:0001217", "UniRef:UniRef50_P12878", "UniRef:UniRef90_P47410" ], "nt": "ATGGCTAAAAAATCATTAAAAGTAAAACAATCCCGTCCCAATAAGTTTAGTGTACGCGACTACACCAGGTGTTTAAGGTGTGGGCGTGCTAGAGCAGTGTTAAGCCACTTTGGTGTGTGTAGGTTGTGTTTCCGTGAACTTGCTTATGCAGGAGCAATCCCAGGAGTTAAAAAAGCATCATGA", "aa": "MAKKSLKVKQSRPNKFSVRDYTRCLRCGRARAVLSHFGVCRLCFRELAYAGAIPGVKKAS", "aa_hexdigest": "985e1a9c2861017fe85276491b751686", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47410", "query_cov": 1.0, "subject_cov": 0.9836065573770492, "identity": 1.0, "score": 120.0, "evalue": 6.41e-35, "valid": true, "gene": "rpsZ", "product": "type Z 30S ribosomal protein S14", "uniref50_id": "UniRef50_P12878", "cog_id": "COG0199", "cog_category": "J", "kegg_orthology_id": "K02954", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0008270", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_P12878", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P12878" ], "product": "Small ribosomal subunit protein uS14B" }, "genes": [ "rpsZ" ], "id": "JPCNHPFPBM_388", "locus": "GCA000292505_00352" }, { "type": "cds", "sequence": "contig_1", "start": 196982, "stop": 197407, "strand": "+", "frame": 2, "gene": "rpsH", "product": "30S ribosomal protein S8", "db_xrefs": [ "COG:COG0096", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "RefSeq:WP_010869359.1", "SO:0001217", "UniParc:UPI000013516D", "UniRef:UniRef100_P47411", "UniRef:UniRef50_Q9PQP6", "UniRef:UniRef90_P47411" ], "nt": "ATGATAATCAATAAAGTTCCCAAAGCCCATTTTGATCCAGTTTCTGATCTTTTCACTAAGATCAACAATGCTAGAAAAGCTAAGCTTTTAACTGTTACCACCATCGCTTCTAAGTTAAAGATAGCTATCTTAGAGATTTTGATTAAAGAGGGCTATTTAGCTAACTATCAGGTGTTGGAAAATAAAACTAAAACCAAAAAACTAGTTAGTTTCACATTAAAATACACCCAAAGAAGGATATGTTCTATTAATGGGGTGAAACAGATCTCAAAACCAGGATTAAGAATCTATCGTTCCTTTGAAAAACTTCCCCTTGTTTTAAATGGTCTTGGTATTGCAATTATCTCCACTAGTGATGGAGTGATGACTGATAAAGTAGCAAGGTTAAAGAAGATTGGTGGGGAGATTTTAGCTTACGTTTGGTAA", "aa": "MIINKVPKAHFDPVSDLFTKINNARKAKLLTVTTIASKLKIAILEILIKEGYLANYQVLENKTKTKKLVSFTLKYTQRRICSINGVKQISKPGLRIYRSFEKLPLVLNGLGIAIISTSDGVMTDKVARLKKIGGEILAYVW", "aa_hexdigest": "d93016cb3156fd323ac12380b7f7223e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013516D", "ncbi_nrp_id": "WP_010869359.1", "uniref100_id": "UniRef100_P47411", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013516D", "RefSeq:WP_010869359.1", "UniRef:UniRef100_P47411" ] }, "ips": { "uniref100_id": "UniRef100_P47411", "uniref90_id": "UniRef90_P47411" }, "psc": { "uniref90_id": "UniRef90_P47411", "gene": "rpsH", "product": "30S ribosomal protein S8", "uniref50_id": "UniRef50_Q9PQP6", "cog_id": "COG0096", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP6" ], "product": "Small ribosomal subunit protein uS8" }, "genes": [ "rpsH" ], "id": "JPCNHPFPBM_389", "locus": "GCA000292505_00353" }, { "type": "cds", "sequence": "contig_1", "start": 197414, "stop": 197875, "strand": "+", "frame": 2, "gene": "rplF", "product": "50S ribosomal protein L6", "db_xrefs": [ "COG:COG0097", "COG:J", "GO:0002181", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625", "SO:0001217", "UniRef:UniRef50_P47412", "UniRef:UniRef90_P47412" ], "nt": "ATGTCAAAAATAGGAAATAGACCAATCAAAATTGATCCTAGTAAAGTGAGTTTAATGCAAACAACAACACTGCTTACTATTAAAGGACCATTAGGGGAAAACACCATTAAACTACCCAAAAACTTACCCTTAAAGTTTGTTGTTGAAAATGACACTATTAAAGTAACTAATAACAACAACTTAAAACAAACTAAGATCTTACACGGTACTTTCAATGCGTTAGTTAACAACGCAGTTATTGGGGTTACCAAGGGTTTTGAAAAGAAACTCATCCTAGTTGGGGTTGGTTATCGTGCTAATGTGGAAGGGCAATTTCTCAACTTACAGTTGGGCTATTCCCATCCTATTAAGGAGTTGATCCCAAACCAACTTACTGTTAAAGTAGAGAAGAACACTGAAATCACCATTAGTGGAATAAAAAAAGAGTTAGTAGGTCAGTTTGCCACTGAAATCAGAAAGTGA", "aa": "MSKIGNRPIKIDPSKVSLMQTTTLLTIKGPLGENTIKLPKNLPLKFVVENDTIKVTNNNNLKQTKILHGTFNALVNNAVIGVTKGFEKKLILVGVGYRANVEGQFLNLQLGYSHPIKELIPNQLTVKVEKNTEITISGIKKELVGQFATEIRK", "aa_hexdigest": "562434f09928b21fc6e22a740fd5f3e7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47412", "query_cov": 1.0, "subject_cov": 0.8315217391304348, "identity": 0.993, "score": 295.0, "evalue": 1.23e-100, "valid": true, "gene": "rplF", "product": "50S ribosomal protein L6", "uniref50_id": "UniRef50_P47412", "cog_id": "COG0097", "cog_category": "J", "go_ids": [ "GO:0002181", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47412", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47412" ], "product": "Large ribosomal subunit protein uL6" }, "genes": [ "rplF" ], "id": "JPCNHPFPBM_390", "locus": "GCA000292505_00354" }, { "type": "cds", "sequence": "contig_1", "start": 197928, "stop": 198317, "strand": "+", "frame": 3, "gene": "rplR", "product": "50S ribosomal protein L18", "db_xrefs": [ "COG:COG0256", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0008097", "GO:0022625", "SO:0001217", "UniRef:UniRef50_P47413", "UniRef:UniRef90_P47413" ], "nt": "TTGTTAGAAAACAAGGTAAAACTGCAGAGGGCAAGAAATAAGATGACAAGAAACGATAAAAGAAGGATTAGACACAAACGGATTGTCAAAAAGATTAGGTTAACTAACCTTAACAACAGGGTTGTACTAATTGTTATCAAGAGTTTAAAAAACATCTCGGTTCAAGCTTGGGACTTTAGTAAGAACGTTGTTTTAACATCAAGTTCCTCACTTCAACTAAAATTAAAAAATGGCAACAAGGAGAATGCTAAACTAGTGGGAATGGATATTGCAACCAAACTCATCAAACTAAACCAAAAGGATGTGGTTTTTGATACTGGGGGTAGTAAGTACCATGGTAGGATTGCTGCTTTAGCAGAAGGAGCGCGAGCTAAGGGTTTAAATTTTTAA", "aa": "MLENKVKLQRARNKMTRNDKRRIRHKRIVKKIRLTNLNNRVVLIVIKSLKNISVQAWDFSKNVVLTSSSSLQLKLKNGNKENAKLVGMDIATKLIKLNQKDVVFDTGGSKYHGRIAALAEGARAKGLNF", "aa_hexdigest": "15e9ca914fcccb25399aefec1d6ff1b5", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47413", "query_cov": 0.8914728682170543, "subject_cov": 1.0, "identity": 1.0, "score": 212.0, "evalue": 2.73e-69, "valid": true, "gene": "rplR", "product": "50S ribosomal protein L18", "uniref50_id": "UniRef50_P47413", "cog_id": "COG0256", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0008097", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47413", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47413" ], "product": "Large ribosomal subunit protein uL18" }, "genes": [ "rplR" ], "id": "JPCNHPFPBM_391", "locus": "GCA000292505_00355" }, { "type": "cds", "sequence": "contig_1", "start": 198322, "stop": 198957, "strand": "+", "frame": 1, "gene": "rpsE", "product": "30S ribosomal protein S5", "db_xrefs": [ "COG:COG0098", "COG:J", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0022627", "KEGG:K02988", "RefSeq:WP_009885854.1", "SO:0001217", "UniParc:UPI000013505F", "UniRef:UniRef100_P47414", "UniRef:UniRef50_Q50301", "UniRef:UniRef90_P47414" ], "nt": "ATGAATGATCAAAAAACTACTAACACTGGCTTGTTAACTTCCACTCTTAAAACCAAGCCCAAACACAACCTTAAACCTTCCAGTGAAGCCATTAAAAAAGCAGTGTCCAAAAAGGAAGGTCATTACAAAAACAAGCGCTTTCAAAAACATAACTTTAATAACAAAAGTGAGTTTGAAGAGAGGATTGTCAAACTCAAACGGATCTCCAAAACCACAAAAGGTGGGAGAAACATGCGCTTTAGTGTCCTTGTTGTTGTTGGTAACAAAAAGGGCAAGGTTGGTTATGGGATTGCTAAGGCATTGGAAGTACCACTTGCCATTAAAAAAGCGATTAAAAAAGCCCATAACTCCATTCATACAGTAGAGATCCATAAGGGTTCAATCTACCACGAAGTGATTGGTAGAAAAGGTGCATCTAAGGTGTTGTTAAAACCTGCACCTTTAGGAACTGGGATCATTGCTGGGGGAGCGATCCGTGCAATTGTAGAGTTAGCTGGTTTTAGTGATATCTATACCAAGAACTTGGGAAGAAACACCCCCATTAACATGATCCATGCCACTATGGATGGGATCTTAAAGCAACTCTCACCCAAAAAAGTGGCATTATTAAGAAATAAACCAATTAGTGATCTATAA", "aa": "MNDQKTTNTGLLTSTLKTKPKHNLKPSSEAIKKAVSKKEGHYKNKRFQKHNFNNKSEFEERIVKLKRISKTTKGGRNMRFSVLVVVGNKKGKVGYGIAKALEVPLAIKKAIKKAHNSIHTVEIHKGSIYHEVIGRKGASKVLLKPAPLGTGIIAGGAIRAIVELAGFSDIYTKNLGRNTPINMIHATMDGILKQLSPKKVALLRNKPISDL", "aa_hexdigest": "7e209050f963e4e037ea509a6ecbdd6c", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013505F", "ncbi_nrp_id": "WP_009885854.1", "uniref100_id": "UniRef100_P47414", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013505F", "RefSeq:WP_009885854.1", "UniRef:UniRef100_P47414" ] }, "ips": { "uniref100_id": "UniRef100_P47414", "uniref90_id": "UniRef90_P47414" }, "psc": { "uniref90_id": "UniRef90_P47414", "gene": "rpsE", "product": "30S ribosomal protein S5", "uniref50_id": "UniRef50_Q50301", "cog_id": "COG0098", "cog_category": "J", "kegg_orthology_id": "K02988", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_Q50301", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50301" ], "product": "Small ribosomal subunit protein uS5" }, "genes": [ "rpsE" ], "id": "JPCNHPFPBM_392", "locus": "GCA000292505_00356" }, { "type": "cds", "sequence": "contig_1", "start": 198963, "stop": 199415, "strand": "+", "frame": 3, "gene": "rplO", "product": "50S ribosomal protein L15", "db_xrefs": [ "COG:COG0200", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625", "KEGG:K02876", "RefSeq:WP_010869361.1", "SO:0001217", "UniParc:UPI0000133C15", "UniRef:UniRef100_P47415", "UniRef:UniRef50_P47415", "UniRef:UniRef90_P47415" ], "nt": "ATGGAACTACACCAATTAAAAAGTGTCTCTAAAAGCCGTAACCACAAGTCCAAAGTGGTAGGTAGGGGCCATGGCTCGGGATTAGGTAAAACATCATCACGTGGTCAAAAGGGACAAAAAGCAAGAAAATCAGGTTTAACTAGGTTAGGTTTTGAAGGGGGACAAACACCCCTTTACCGCCGGTTGCCTAAGTATGGGGTTGCTAACAAAGGGATCTTAAAAAAAAGGTGGGTTGTTTTAAATTTGAACAAAGTTGCTAAACTCAATCTCAAAACAGTTACTAGAGCAACTTTGATTGAAAAAAAGGTAATTAGTAAAAAAAATAACCTCCCTTTGAAGTTAATTGGGAACACAAAACTCACTACTCCCATCCACTTTGAAGTGCAAAAAATCTCCAAAAATGCTTTAAATGCAGTGCAAACTAGCAAAGGTAGTGTGAAAATTATCACCTAA", "aa": "MELHQLKSVSKSRNHKSKVVGRGHGSGLGKTSSRGQKGQKARKSGLTRLGFEGGQTPLYRRLPKYGVANKGILKKRWVVLNLNKVAKLNLKTVTRATLIEKKVISKKNNLPLKLIGNTKLTTPIHFEVQKISKNALNAVQTSKGSVKIIT", "aa_hexdigest": "4c3312aadfabef538e5148365a549821", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133C15", "ncbi_nrp_id": "WP_010869361.1", "uniref100_id": "UniRef100_P47415", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133C15", "RefSeq:WP_010869361.1", "UniRef:UniRef100_P47415" ] }, "ips": { "uniref100_id": "UniRef100_P47415", "uniref90_id": "UniRef90_P47415" }, "psc": { "uniref90_id": "UniRef90_P47415", "gene": "rplO", "product": "50S ribosomal protein L15", "uniref50_id": "UniRef50_P47415", "cog_id": "COG0200", "cog_category": "J", "kegg_orthology_id": "K02876", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47415" ], "product": "Large ribosomal subunit protein uL15" }, "genes": [ "rplO" ], "id": "JPCNHPFPBM_393", "locus": "GCA000292505_00357" }, { "type": "cds", "sequence": "contig_1", "start": 199415, "stop": 200842, "strand": "+", "frame": 2, "gene": "secY", "product": "preprotein translocase subunit SecY", "db_xrefs": [ "COG:COG0201", "COG:U", "GO:0005048", "GO:0005886", "GO:0006616", "GO:0008320", "GO:0015031", "GO:0016020", "GO:0031522", "GO:0043952", "RefSeq:WP_009885855.1", "SO:0001217", "UniParc:UPI00001357E5", "UniRef:UniRef100_P47416", "UniRef:UniRef50_O52351", "UniRef:UniRef90_P47416" ], "nt": "ATGCAAACTGTTTCTTCACCCAAACAAAAACTTAACTTTGGTCAAAGGTTACTAACTCTATTACAGAACCGTGACTTTATGGTGTCACTGGTTTTAACAGTGGTACTTTTAATCTTGTTTAGGGTGTTAGCAATTATCCCCTTACCAGGGATTAGGATTAATGAGAGTGTCTTGGATAGAAATTCCAATGACTTTTTTTCACTTTTTAACTTACTTGGGGGTGGGGGATTAAACCAGCTATCGTTGTTTGCAGTTGGGATCAGTCCTTATATCTCAGCCCAAATCATCATGCAACTGCTTTCAACTGATCTAATTCCTCCACTTTCAAAGCTAGTTAACAGTGGGGAAGTGGGGCGAAGAAAGATTGAGATGATCACAAGAATTATCACCTTACCCTTTGCTTTAGTGCAAGCATTTGCTGTGATCCAAATTGCTACTAATGCAGGCACTGGTTCAAGTCCGATTAGTTTAGCTAATAGTGGCAGTGAGTTTATTGCTTTTTATATTATTGCTATGACTGCAGGGACTTATATGGCAGTGTTTTTGGGTGATACTATCTCCAAAAAAGGGGTTGGTAATGGGATTACTTTGTTAATTCTCTCAGGGATTTTATCCCAACTCCCCCAGGGCTTTATTGCTGCTTACAATGTTTTGAGTGGGATAGTAATTACTCTAACCCCACAGTTAACTGCAGCAATTAGCTTCTTTATCTATTTCTTAGCATTCTTAGTTTTACTGTTTGCCACTACCTTTATCACCCAAGCGACCAGAAAGATTCCCATCCAACAATCAGGACAAGGGTTGGTTAGTGAAGTCAAAACCTTACCTTATTTGCCTATTAAGGTGAATGCTGCTGGGGTGATCCCTGTCATCTTTGCATCCAGTATTATGTCTATCCCTGTGACCATTGCCCAGTTTCAACCCCAAACTGAGTCACGGTGGTTTGTGGAGGATTACCTATCACTTTCAACACCCGTAGGGATCTTTTTATATGCAGTTTTGGTTATCCTTTTTTCCTTTTTTTACAGTTACATCCAGATTAACCCAGAACGGTTAGCTAAGAACTTTGAAAAATCTGGCAGATTTATCCCAGGGATTCGACCGGGCAATGATACAGAGAAACACATTGCGCGGGTGTTAATAAGGATTAACTTTATAGGTGCTCCTTTTTTAACTGTTATTGCTATTATCCCTTACATTGTTTCTTATTTCATTAGGTTACCTAACTCCTTGAGTTTAGGGGGGACGGGGATTATTATTATTGTTACTGCTGTAGTTGAATTTATCAGTGCACTGCGTTCAGCTGCTACTGCTACTAACTACCAACAACTAAGGAGAAACTTAGCAATTGAAGTGCAACAAACAGCTAAACAAGATAGTCTAGAGCAGCTTCAAAAAGAAGCACCAGGGATTGGTAACCTATGGTAG", "aa": "MQTVSSPKQKLNFGQRLLTLLQNRDFMVSLVLTVVLLILFRVLAIIPLPGIRINESVLDRNSNDFFSLFNLLGGGGLNQLSLFAVGISPYISAQIIMQLLSTDLIPPLSKLVNSGEVGRRKIEMITRIITLPFALVQAFAVIQIATNAGTGSSPISLANSGSEFIAFYIIAMTAGTYMAVFLGDTISKKGVGNGITLLILSGILSQLPQGFIAAYNVLSGIVITLTPQLTAAISFFIYFLAFLVLLFATTFITQATRKIPIQQSGQGLVSEVKTLPYLPIKVNAAGVIPVIFASSIMSIPVTIAQFQPQTESRWFVEDYLSLSTPVGIFLYAVLVILFSFFYSYIQINPERLAKNFEKSGRFIPGIRPGNDTEKHIARVLIRINFIGAPFLTVIAIIPYIVSYFIRLPNSLSLGGTGIIIIVTAVVEFISALRSAATATNYQQLRRNLAIEVQQTAKQDSLEQLQKEAPGIGNLW", "aa_hexdigest": "6e776e11b4f501d8e4e80c02c2838412", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001357E5", "ncbi_nrp_id": "WP_009885855.1", "uniref100_id": "UniRef100_P47416", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001357E5", "RefSeq:WP_009885855.1", "UniRef:UniRef100_P47416" ] }, "ips": { "uniref100_id": "UniRef100_P47416", "uniref90_id": "UniRef90_P47416" }, "psc": { "uniref90_id": "UniRef90_P47416", "gene": "secY", "product": "preprotein translocase subunit SecY", "uniref50_id": "UniRef50_O52351", "cog_id": "COG0201", "cog_category": "U", "go_ids": [ "GO:0005048", "GO:0005886", "GO:0006616", "GO:0008320", "GO:0015031", "GO:0016020", "GO:0031522", "GO:0043952" ] }, "pscc": { "uniref50_id": "UniRef50_O52351", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O52351" ], "product": "Protein translocase subunit SecY" }, "genes": [ "secY" ], "id": "JPCNHPFPBM_394", "locus": "GCA000292505_00358" }, { "type": "cds", "sequence": "contig_1", "start": 200836, "stop": 201480, "strand": "+", "frame": 1, "gene": "adk", "product": "Adenylate kinase", "db_xrefs": [ "COG:COG0563", "COG:F", "EC:2.7.4.3", "GO:0004017", "GO:0005524", "GO:0005737", "GO:0008270", "GO:0043231", "GO:0044209", "RefSeq:WP_014894602.1", "SO:0001217", "UniParc:UPI00027B3670", "UniRef:UniRef100_UPI00027B3670", "UniRef:UniRef50_P47417", "UniRef:UniRef90_P47417" ], "nt": "ATGGTAGCACAGTTTAATAAGTTCATTATCTTAGGACCCCCAGGGGCAGGAAAAGGTACAGTTTGTAAACTGCTTAGCAAAACAACTAAGTTAGTCCATATTGCTAGTGGTGATCTGTTTAGAGAAGCCATTAAAAACCAGAGTGTTATTGGTAGAAAGATTGCAGCAATTATCAGTCAGGGTGGTTATGTTGATGATACCACTACTAACCAGCTTGTTTATGAATATATCACTACCAATCCATTACCAAATGGTTTTATCTTAGATGGTTATCCAAGAACAGAGAACCAGCTTGATTTTCTAAATATTAAACTAACCATTGACATGGTCTTTGAACTAGTTGTTAGTGATCTGAATAAACTGATTACACGGATTGATAACAGGGTTATTTGTAACAACTGTAACAGTGTTTATAACTTGCTTTTTCAAAAACCACTAGTTGAAAATAGGTGTGATCAGTGTTCAGCTAAACTAGTGAAAAGGAGTGATGATAACAAAGCAGTGGTCAAAGCAAGGATGAAGTTATATCAACAAACAATTCAACCAATCCACACTTACTTTTTCAACAAACAACTTTTAGTACAAATTGATTGCTTTTTACCACTAGAAGAACAACTCAAGACAATCAAACAATTTATTAGATAA", "aa": "MVAQFNKFIILGPPGAGKGTVCKLLSKTTKLVHIASGDLFREAIKNQSVIGRKIAAIISQGGYVDDTTTNQLVYEYITTNPLPNGFILDGYPRTENQLDFLNIKLTIDMVFELVVSDLNKLITRIDNRVICNNCNSVYNLLFQKPLVENRCDQCSAKLVKRSDDNKAVVKARMKLYQQTIQPIHTYFFNKQLLVQIDCFLPLEEQLKTIKQFIR", "aa_hexdigest": "9e637a2168ce2cdadc93c542d242ffb3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3670", "ncbi_nrp_id": "WP_014894602.1", "uniref100_id": "UniRef100_UPI00027B3670", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3670", "RefSeq:WP_014894602.1", "UniRef:UniRef100_UPI00027B3670" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3670", "uniref90_id": "UniRef90_P47417" }, "psc": { "uniref90_id": "UniRef90_P47417", "gene": "adk", "product": "Adenylate kinase", "ec_ids": [ "2.7.4.3" ], "uniref50_id": "UniRef50_P47417", "cog_id": "COG0563", "cog_category": "F", "go_ids": [ "GO:0005737", "GO:0043231", "GO:0004017", "GO:0005524", "GO:0008270", "GO:0044209" ] }, "pscc": { "uniref50_id": "UniRef50_P47417", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47417" ], "product": "Adenylate kinase" }, "genes": [ "adk" ], "id": "JPCNHPFPBM_395", "locus": "GCA000292505_00359" }, { "type": "cds", "sequence": "contig_1", "start": 201483, "stop": 202229, "strand": "+", "frame": 3, "gene": "map", "product": "type I methionyl aminopeptidase", "db_xrefs": [ "COG:COG0024", "COG:J", "EC:3.4.11.18", "GO:0004239", "GO:0005829", "GO:0006508", "GO:0046872", "GO:0070006", "KEGG:K01265", "RefSeq:WP_009885857.1", "SO:0001217", "UniParc:UPI0000125A04", "UniRef:UniRef100_P47418", "UniRef:UniRef50_P47418", "UniRef:UniRef90_P47418" ], "nt": "ATGATCTATCTCAAATCTGCAAATGAAGTTGCAGGGATTAAAAAAGCATGTGCAATCTTCAAAGCAGTTAAGGCATATTTTACAATTGAAAAGTTACTTGGCAAAAAGTTGGTTACCATTGATCGTTTAATCAAACAATTCATTGAACAAAAACAAGCTAAATGTGCGTTTCATGGTTATCTAGGTTTCCCTGGTTTTAACTGTCTATCGTTAAACCAAACGGTTATCCATGGGGTTGCAGATCAAACTGTTTTTAAAGATAGTGATAAACTAACGCTTGACATTGGGATAGACTATCATGGTTATCTTTGTGATGCAGCTTTCACTTTACTTGGTAATAAAGCTGATCCAAAGGCAGTAAAACTGTTAAATGATGTTGAACAAGCATTTAGTAAGGTAATTGAACCTGAGCTATTTGTTAACAATCCGATTGGTAATTTATCCAATGCGATCCAAACTTACTTTGAAAACAAGGGCTATTTTCTTGTCAAAGAGTTTGGGGGTCATGGTTGTGGGATTAAGATCCATGAAGATCCTTTAATCTTAAACTGGGGAGAGAAAAACCAGGGCGTTAGGTTACAAGAGGGGATGGTAATCTGTATTGAACCGATGGTTATGACTGATAGTAGTGAGATAACAATGGCAGCTAACAACTGGAATGTACTAACTTTAAAGAGTAAGTTTAACTGTCATGTGGAACAGATGTATCACATCACAAACAACGGCTTTGAATGTTTAACTAACTAA", "aa": "MIYLKSANEVAGIKKACAIFKAVKAYFTIEKLLGKKLVTIDRLIKQFIEQKQAKCAFHGYLGFPGFNCLSLNQTVIHGVADQTVFKDSDKLTLDIGIDYHGYLCDAAFTLLGNKADPKAVKLLNDVEQAFSKVIEPELFVNNPIGNLSNAIQTYFENKGYFLVKEFGGHGCGIKIHEDPLILNWGEKNQGVRLQEGMVICIEPMVMTDSSEITMAANNWNVLTLKSKFNCHVEQMYHITNNGFECLTN", "aa_hexdigest": "776ed85c8b07d493b4167d8c962b87b0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000125A04", "ncbi_nrp_id": "WP_009885857.1", "uniref100_id": "UniRef100_P47418", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000125A04", "RefSeq:WP_009885857.1", "UniRef:UniRef100_P47418" ] }, "ips": { "uniref100_id": "UniRef100_P47418", "uniref90_id": "UniRef90_P47418" }, "psc": { "uniref90_id": "UniRef90_P47418", "gene": "map", "product": "type I methionyl aminopeptidase", "ec_ids": [ "3.4.11.18" ], "uniref50_id": "UniRef50_P47418", "cog_id": "COG0024", "cog_category": "J", "kegg_orthology_id": "K01265", "go_ids": [ "GO:0004239", "GO:0005829", "GO:0006508", "GO:0046872", "GO:0070006" ] }, "pscc": { "uniref50_id": "UniRef50_P47418", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47418" ], "product": "Methionine aminopeptidase" }, "genes": [ "map" ], "id": "JPCNHPFPBM_396", "locus": "GCA000292505_00360" }, { "type": "cds", "sequence": "contig_1", "start": 202229, "stop": 202441, "strand": "+", "frame": 2, "gene": "infA", "product": "translation initiation factor IF-1", "db_xrefs": [ "COG:COG0361", "COG:J", "GO:0003743", "GO:0005829", "GO:0006413", "GO:0019843", "GO:0043022", "KEGG:K02518", "RefSeq:WP_009885858.1", "SO:0001217", "UniParc:UPI000012D262", "UniRef:UniRef100_P47419", "UniRef:UniRef50_Q50298", "UniRef:UniRef90_P47419" ], "nt": "ATGAAAAACGATAAACTCTTTCTAACAGGTAAGATACTGGAAATTATCCATGGTGATAAGTACCGGGTGATGCTTGAAAACAATGTTGAGGTTGATGCACATCTAGCAGGTAAAATGAAGATGAAAAGAACCAAGATTCTCCCTGGGGATGTTGTTGAGGTGGAATTTTCTCCCTATGATTTGAAACTAGGTAGGATAACCCAAAGAAAATAA", "aa": "MKNDKLFLTGKILEIIHGDKYRVMLENNVEVDAHLAGKMKMKRTKILPGDVVEVEFSPYDLKLGRITQRK", "aa_hexdigest": "cb34f5de7123f10488fac58fbf88077f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012D262", "ncbi_nrp_id": "WP_009885858.1", "uniref100_id": "UniRef100_P47419", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012D262", "RefSeq:WP_009885858.1", "UniRef:UniRef100_P47419" ] }, "ips": { "uniref100_id": "UniRef100_P47419", "uniref90_id": "UniRef90_P47419" }, "psc": { "uniref90_id": "UniRef90_P47419", "gene": "infA", "product": "translation initiation factor IF-1", "uniref50_id": "UniRef50_Q50298", "cog_id": "COG0361", "cog_category": "J", "kegg_orthology_id": "K02518", "go_ids": [ "GO:0003743", "GO:0005829", "GO:0006413", "GO:0019843", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_Q50298", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50298" ], "product": "Translation initiation factor IF-1" }, "genes": [ "infA" ], "id": "JPCNHPFPBM_397", "locus": "GCA000292505_00361" }, { "type": "cds", "sequence": "contig_1", "start": 202565, "stop": 202822, "strand": "+", "frame": 2, "gene": null, "product": "30S ribosomal protein S13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A535XWH1" ], "nt": "ATGGCACGAATCTTAGGGATTGATATCCCCAACCAAAAACGGATCGAGATAGCTTTAACATACATCTTTGGGATTGGTTTGTCAAGTGCAAAAACAATCTTAAAAAAAGCAAAGATTAACCCTGATAAACGCGTTAAAGATCTGAGTGAAGAGGAACTTGTTGCGATTAGAAACGCAGCAAGCGGTTACAAGATTGAGGGTGATTTGAGAAGAGAGATTGCTTTAAACATCAAACACCTAACAGAGATCGGTTCTTGA", "aa": "MARILGIDIPNQKRIEIALTYIFGIGLSSAKTILKKAKINPDKRVKDLSEEELVAIRNAASGYKIEGDLRREIALNIKHLTEIGS", "aa_hexdigest": "11c6e5322ee6e5b4fc1e95a948b562ec", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0031B5D25D", "query_cov": 1.011764705882353, "subject_cov": 0.9885057471264368, "identity": 0.57, "score": 95.5, "evalue": 4.27e-24, "valid": false, "product": "30S ribosomal protein S13", "uniref50_id": "UniRef50_A0A535XWH1", "kegg_orthology_id": "K02952" }, "pscc": { "uniref50_id": "UniRef50_A0A535XWH1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A535XWH1" ], "product": "30S ribosomal protein S13" }, "genes": [], "id": "JPCNHPFPBM_398", "locus": "GCA000292505_00362" }, { "type": "cds", "sequence": "contig_1", "start": 203125, "stop": 203304, "strand": "+", "frame": 1, "gene": "rpsK", "product": "30S ribosomal protein S11", "db_xrefs": [ "COG:COG0100", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "KEGG:K02948", "SO:0001217", "UniRef:UniRef50_P47422", "UniRef:UniRef90_P47422" ], "nt": "GTGGCTAAAACTGTGAAAGAGATGGGAATGGGGAGTGTTAAGATGTATCTGAAGGGAACAGGTAGAGGAAAAGACACCACGATTAGAAGCTTTGCTAATGCTGGGATTACGATCACAGAAATCAATGAAAAAACCCCTATTCCCCACAATGGCTGCAAGCCTCCTAAGCGTCCGCGCTAA", "aa": "MAKTVKEMGMGSVKMYLKGTGRGKDTTIRSFANAGITITEINEKTPIPHNGCKPPKRPR", "aa_hexdigest": "1ffbae663cd83fb40a4b2d12bf80cca3", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_K2BZH6", "query_cov": 0.9322033898305084, "subject_cov": 0.8461538461538461, "identity": 0.509, "score": 67.4, "evalue": 1.11e-13, "valid": false, "product": "Small ribosomal subunit protein uS11", "uniref50_id": "UniRef50_K2BZH6" }, "genes": [ "rpsK" ], "truncated": "5-prime", "pseudogene": { "start": 202939, "stop": 203304, "observations": { "mutation": [ 203047 ], "stop": [ 203047 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47422", "query_cov": 1.0, "subject_cov": 0.48760330578512395, "identity": 0.983, "score": 123.0, "evalue": 2.97e-35, "gene_start": 1, "gene_end": 59, "reference_start": 63, "reference_end": 121, "reference_sequence": "MAKKKKINVPSGLIHVSCSPNNTIVSATDPSGNVLCWASSGTVGFKGFRKKTPYSAGVAADKVAKTVKEMGMGSVKMYLKGTGRGKDTTIRSFANAGITITEINEKTPIPHNGCKPPKRPR" }, "paralog": false, "identity": 0.9917355371900827, "score": 244.0, "evalue": 1.92e-85, "description": "Internal stop codon at 203047. Nonsense mutation around 203047.", "psc": { "uniref90_id": "UniRef90_P47422", "gene": "rpsK", "product": "30S ribosomal protein S11", "uniref50_id": "UniRef50_P47422", "cog_id": "COG0100", "cog_category": "J", "kegg_orthology_id": "K02948", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47422", "UniRef:UniRef50_P47422", "COG:COG0100", "COG:J", "KEGG:K02948", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P47422", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47422" ], "product": "Small ribosomal subunit protein uS11" } }, "id": "JPCNHPFPBM_399", "locus": "GCA000292505_00363" }, { "type": "cds", "sequence": "contig_1", "start": 203320, "stop": 203649, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01193.29" ], "nt": "ATGGAAAAATTTTTAAAGTACGAAATTAAGGTTAACAACAACCAACCAACCAACACTAACCCTAACTATGGGATCTTTGAAGTAGCACCGTTAGAATCAGGATTTGGGATTACCATTGGTAATGCGATGCGCCGAGTGTTACTTAGTTGTATCCCAGGCGCTAGTGTGTTTGCCATTGCCATTAGTGGGGTAAAACAAGAGTTTAGTAATGTGGAGGGTGTGTTGGAAGATGTGACTGAAATGGTGTTAAACTTCAAGCAACTAGTGGTGAGAATCTCTGATCTTTTGTTTGAAGATGGGGAGATGATCGAACCACCCTTAGAAAGGTGA", "aa": "MEKFLKYEIKVNNNQPTNTNPNYGIFEVAPLESGFGITIGNAMRRVLLSCIPGASVFAIAISGVKQEFSNVEGVLEDVTEMVLNFKQLVVRISDLLFEDGEMIEPPLER", "aa_hexdigest": "b0289ee9d10a5f7ece465eca25a24da6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01193.29", "name": "RNA_pol_L", "length": 43, "aa_cov": 0.3944954128440367, "hmm_cov": 0.6976744186046512, "evalue": 2.2884642015236258e-10, "score": 35.22905731201172, "start": 30, "stop": 72 } ], "seq_stats": { "molecular_weight": 12135.856599999996, "isoelectric_point": 4.564591789245606 }, "id": "JPCNHPFPBM_400", "locus": "GCA000292505_00364" }, { "type": "cds", "sequence": "contig_1", "start": 203725, "stop": 204306, "strand": "+", "frame": 1, "gene": "rpoA", "product": "DNA-directed RNA polymerase subunit alpha", "db_xrefs": [ "COG:COG0202", "COG:K", "EC:2.7.7.6", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0005737", "GO:0006351", "GO:0046983", "SO:0001217", "UniRef:UniRef50_P47423", "UniRef:UniRef90_P47423" ], "nt": "GTGATTAATAAGGACCTTTATCTCTTCTCTTTACAAAAGGACATGAAACTAACAGTCAGTGTTTATGTTAAACAGGGTAGGGGCTTTACTAGCTTTCTTGAAAACAGAGAATTGATCAATTCGCTTGGCATTATTGCTACAGATGCTAACTTTTCCCCGGTTTTACACTGTGGTTATGAAGTTCAAGAGGTGAAAACTTCCAAACAAAAGTTAACTGACCATCTCACCTTTAAGATTGCTACTAACGGTGCAATTAAAGCAGTGGATGCGTTTGCTATGGCAGCAAAGATCCTAATTGAACACTTAAACCCAATTGTAAGTGTCAATGAGTCAATTAAGAATTTAACAATTATCCAAGAGAAAGCAGAGGAAAGAAAGGTGAAATCATTTGCCAAGCAAATTGAAGAACTTGACTTTACTGTTAGAACCTTTAACTGTTTGAAAAGAAGTGGGATCCACACACTCCAAGAGTTACTATCAAAGTCATTAACTGACATTAGAGAGATTAGAAACCTAGGTAAGAAATCAGAACGGGAGATTATCAAAAAGGTGCAAGAGTTAGGTTTAAAACTCCGTTCTTAA", "aa": "MINKDLYLFSLQKDMKLTVSVYVKQGRGFTSFLENRELINSLGIIATDANFSPVLHCGYEVQEVKTSKQKLTDHLTFKIATNGAIKAVDAFAMAAKILIEHLNPIVSVNESIKNLTIIQEKAEERKVKSFAKQIEELDFTVRTFNCLKRSGIHTLQELLSKSLTDIREIRNLGKKSEREIIKKVQELGLKLRS", "aa_hexdigest": "a24a1ade8fe723a049d2a2f110ea160a", "start_type": "GTG", "rbs_motif": null, "genes": [ "rpoA" ], "truncated": "5-prime", "pseudogene": { "start": 203425, "stop": 204306, "observations": { "mutation": [ 203647 ], "stop": [ 203647 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47423", "query_cov": 0.9948186528497409, "subject_cov": 0.5853658536585366, "identity": 0.995, "score": 359.0, "evalue": 3.34e-123, "gene_start": 2, "gene_end": 193, "reference_start": 137, "reference_end": 328, "reference_sequence": "MEKFLKYEIKVNNNQPTNTNPNYGIFEVAPLESGFGITIGNAMRRVLLSCIPGASVFAIAISGVKQEFSNVEGVLEDVTEMVLNFKQLVVRISDLLFEDGEMIEPPLERWPVLKVTAEKKGAVYAKDLECPAGFEVINKDLYLFSLQKDMKLTVSVYVKQGRGFTSFLENRELINSLGIIATDANFSPVLHCGYEVQEVKTSKQKLTDHLTFKIATNGAIKAVDAFAMAAKILIEHLNPIVSVNESIKNLTIIQEKAEERKVKSFAKQIEELDFTVRTFNCLKRSGIHTLQELLSKSLTDIREIRNLGKKSEREIIKKVQELGLKFRS" }, "paralog": false, "identity": 0.9931740614334471, "score": 557.0, "evalue": 1.35e-203, "description": "Internal stop codon at 203647. Nonsense mutation around 203647.", "psc": { "uniref90_id": "UniRef90_P47423", "gene": "rpoA", "product": "DNA-directed RNA polymerase subunit alpha", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P47423", "cog_id": "COG0202", "cog_category": "K", "go_ids": [ "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0005737", "GO:0006351", "GO:0046983" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47423", "EC:2.7.7.6", "UniRef:UniRef50_P47423", "COG:COG0202", "COG:K", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0005737", "GO:0006351", "GO:0046983" ] }, "pscc": { "uniref50_id": "UniRef50_P47423", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47423" ], "product": "DNA-directed RNA polymerase subunit alpha" } }, "id": "JPCNHPFPBM_401", "locus": "GCA000292505_00365" }, { "type": "cds", "sequence": "contig_1", "start": 204364, "stop": 204690, "strand": "+", "frame": 1, "gene": "rplQ", "product": "50S ribosomal protein L17", "db_xrefs": [ "COG:COG0203", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "KEGG:K02879", "SO:0001217", "UniRef:UniRef50_P47424", "UniRef:UniRef90_P47424" ], "nt": "ATGACAGTGCGTCAGCAAGTGAGTGCAGTGTTAAGTTATGGGAAGATTCAAACCACTTTAAAAAAAGCTAAGAACACCCAAAAAAGGTTAGAGAAGATTATTACCATTGCTAAAGTTGATAACTTTAACAACCGTAGGGCTGTTAAAAAGTGGTTATTAAATACCAATTCATTGGATGTAGATCAACTCACTAACCACCTTTTTAAAAAAGTAGCACCACGTTTTTTAAAGCGTAATGGTGGTTATAGTAGAGTGTTAAAGTTGGGAGTTAGAAGGGGTGATAGTACTGAAATGGTGATCTTACAGCTGATAGATGCTACCAACTAA", "aa": "MTVRQQVSAVLSYGKIQTTLKKAKNTQKRLEKIITIAKVDNFNNRRAVKKWLLNTNSLDVDQLTNHLFKKVAPRFLKRNGGYSRVLKLGVRRGDSTEMVILQLIDATN", "aa_hexdigest": "307fc3639b9ffd34591d98dd3daacd16", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47424", "query_cov": 1.0, "subject_cov": 0.8780487804878049, "identity": 0.991, "score": 206.0, "evalue": 2.91e-67, "valid": true, "gene": "rplQ", "product": "50S ribosomal protein L17", "uniref50_id": "UniRef50_P47424", "cog_id": "COG0203", "cog_category": "J", "kegg_orthology_id": "K02879", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47424", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47424" ], "product": "Large ribosomal subunit protein bL17" }, "genes": [ "rplQ" ], "id": "JPCNHPFPBM_402", "locus": "GCA000292505_00366" }, { "type": "cds", "sequence": "contig_1", "start": 204677, "stop": 205489, "strand": "+", "frame": 2, "gene": "ecfA1", "product": "energy-coupling factor transporter ATPase", "db_xrefs": [ "COG:COG1122", "COG:PR", "EC:7.-.-.-", "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190", "SO:0001217", "UniRef:UniRef50_P47425", "UniRef:UniRef90_P47425" ], "nt": "ATGCTACCAACTAAACAAGCTGCTTGTAGTTTTATTAATGTTGCTTTTTCCTATAATGAACTGCCATTAATTAGGGAACTATCTTTTAGTGTTTATGAGGGGGAATATGTTTGTATTGTTGGTCATAATGGCAGTGGTAAATCAACCATTTCCAAACTGTTAACCGGGTTATTAAAACCCCAGTCAGGTGAGATTAAGATCTTTGGTAAAACAGTTGATTTTGATAATGTTAGTTACTTGAGAAATAACATTGGGATCATCTTTCAAAACCCTGATAACCAGTTTATTGGGATCACTGTTGAAGATGACATTGCCTTTGGGCTTGAAAACAAGTGTTTTTCAAGACAGAAGATAAAAGCCATTATTGATGAAGTGACATTACAAACCCAAACTGATGGGTTTATTAAACAAGAACCCCATAACCTATCAGGCGGACAAAAACAACGGGTAGCAATTGCATCTGTTTTAGCACTAAATCCTGCTATTATCATCTTTGATGAATCAACTGCGATGTTAGATCCTAAAGCTAAAAAAACGATTAAGCAGTTTATGGTTAAACTATCTAAACAGGGCAAGTGTGTGATCTCAATTACCCATGATATGGAAGAAGTTACTAAAGCTGATAAGGTGTTAGTAATGAATGAGGGCAAACTGATCAAACAAGGCAAACCTGTTGAAGTTTTCACTAGTGAACAAGAGTTACAAAAAATCCGTTTAGACATCCCTTTTTCACTCAGTCTTTCAACCAAGATAAAAGGGATCACTAGTACAATTGATTACCAAACCCTGATTAAATCAATTGCCAAGCTGTGA", "aa": "MLPTKQAACSFINVAFSYNELPLIRELSFSVYEGEYVCIVGHNGSGKSTISKLLTGLLKPQSGEIKIFGKTVDFDNVSYLRNNIGIIFQNPDNQFIGITVEDDIAFGLENKCFSRQKIKAIIDEVTLQTQTDGFIKQEPHNLSGGQKQRVAIASVLALNPAIIIFDESTAMLDPKAKKTIKQFMVKLSKQGKCVISITHDMEEVTKADKVLVMNEGKLIKQGKPVEVFTSEQELQKIRLDIPFSLSLSTKIKGITSTIDYQTLIKSIAKL", "aa_hexdigest": "716e47803ca04e2c41487dbc3ecc63fa", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47425", "query_cov": 1.0, "subject_cov": 0.9854014598540146, "identity": 0.9890000000000001, "score": 517.0, "evalue": 3.98e-185, "valid": true, "gene": "ecfA1", "product": "energy-coupling factor transporter ATPase", "ec_ids": [ "7.-.-.-" ], "uniref50_id": "UniRef50_P47425", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190" ] }, "pscc": { "uniref50_id": "UniRef50_P47425", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47425" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA1" }, "genes": [ "ecfA1" ], "id": "JPCNHPFPBM_403", "locus": "GCA000292505_00367" }, { "type": "cds", "sequence": "contig_1", "start": 205486, "stop": 206400, "strand": "+", "frame": 1, "gene": "ecfA2", "product": "energy-coupling factor transporter ATPase", "db_xrefs": [ "COG:COG1122", "COG:PR", "EC:3.6.3.-", "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190", "RefSeq:WP_009885865.1", "SO:0001217", "UniParc:UPI00001393F5", "UniRef:UniRef100_P47426", "UniRef:UniRef50_Q50293", "UniRef:UniRef90_P47426" ], "nt": "GTGAAAAAAAAGATAGTCCCAATTAACCCTTTAAAAGCAGATGAGATTTTAGCAGTTAGTCACTTATCATGTGTTTTTAACAGTAAAACTAACAATCCCATTAAGGTGATTGATGATTTTTCCTATACCTTTCAAAAGAACCAAATTTACTGTATTATTGGTGATAGTGGCAGTGGTAAATCAACCCTTGTTAACCACTTCAATGGGTTGATAAAACCCAACCAAGGTGATATTTGGGTTAAAGATATCTATATTGGTGCTAAACAACGCAAGATTAAGAACTTTAAAAAACTGCGAAAAACTATCTCAATTGTTTTCCAGTTTCCTGAGTACCAATTGTTTAAAGATACCGTGGAAAAAGACATTATGTTTGGTCCAGTAGCATTAGGTCAATCCAAGTATGATGCGCGCCAAAAAGCGGCTTATTATCTGGAGATGATGGGGTTAAAATACCCCTTTTTAGAACGTAATCCCTTTGAATTGAGTGGGGGGCAGAAAAGAAGGGTAGCGATTGCTGGTATACTTGCAATTGAACCAGAAATTCTAATCTTTGATGAACCAACTGCTGGGCTTGATCCTGAAGGGGAAAGGGAGATGATGCAGTTAATTAAAACTGCCAAACAACAACAAAGAACGGTATTTATGATCACCCACCAGATGGAAAATGTCCTTGAGGTGGCTGATGTGGTTTTGGTTTTAGCTAAGGGTAAACTAGTAAAAGCTGCTAGTCCATATGAAGTGTTTATGGACCAAACTTTCCTTGAAAAAACAACGATTGTTCTCCCCCCTGTGATCCAAGTGATCAAAGATCTAATTGCGATTAATGCTCACTTTAATAAGTTAATTGAGTTGCAACCAAAGAACCTAGAACAGCTTGCATCAGCAATTAACAAGACTATAGCAAACCATGGATAA", "aa": "MKKKIVPINPLKADEILAVSHLSCVFNSKTNNPIKVIDDFSYTFQKNQIYCIIGDSGSGKSTLVNHFNGLIKPNQGDIWVKDIYIGAKQRKIKNFKKLRKTISIVFQFPEYQLFKDTVEKDIMFGPVALGQSKYDARQKAAYYLEMMGLKYPFLERNPFELSGGQKRRVAIAGILAIEPEILIFDEPTAGLDPEGEREMMQLIKTAKQQQRTVFMITHQMENVLEVADVVLVLAKGKLVKAASPYEVFMDQTFLEKTTIVLPPVIQVIKDLIAINAHFNKLIELQPKNLEQLASAINKTIANHG", "aa_hexdigest": "4db8cad8c5695dfd3904d297caf5ae55", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001393F5", "ncbi_nrp_id": "WP_009885865.1", "uniref100_id": "UniRef100_P47426", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001393F5", "RefSeq:WP_009885865.1", "UniRef:UniRef100_P47426" ] }, "ips": { "uniref100_id": "UniRef100_P47426", "uniref90_id": "UniRef90_P47426" }, "psc": { "uniref90_id": "UniRef90_P47426", "gene": "ecfA2", "product": "energy-coupling factor transporter ATPase", "ec_ids": [ "3.6.3.-" ], "uniref50_id": "UniRef50_Q50293", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0016887", "GO:0042626", "GO:0043190" ] }, "pscc": { "uniref50_id": "UniRef50_Q50293", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50293" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA2" }, "genes": [ "ecfA2" ], "id": "JPCNHPFPBM_404", "locus": "GCA000292505_00368" }, { "type": "cds", "sequence": "contig_1", "start": 206393, "stop": 206728, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAACTTTATTAATGGCTATATCCCAAGAAACAGCTTTGTTCACAAGCTGCATCCAACTACTAAACTAGTAATCTTTTTACTGTTAGTTATCTTGGTATTTGTACCAATTGGCTTTGTTTTTCAAAGTGTTATCTTTCTTTTTGTTACCTTTGTTTTTTTTATTGCTAAACTCCCGGGGCGGTTTTACAGTTCAGCAATTAAGTCAATTACGCTGTTATTTCTCTTGTTGTTATTTGTAAACTGGTTTACCTTTCGTGATCCAGGGTTTTATCTTACTAGTGATCAACTTAACAGTTTACCAGCCATTGATAACAGCAAGTTTAGCTTTTGA", "aa": "MDNFINGYIPRNSFVHKLHPTTKLVIFLLLVILVFVPIGFVFQSVIFLFVTFVFFIAKLPGRFYSSAIKSITLLFLLLLFVNWFTFRDPGFYLTSDQLNSLPAIDNSKFSF", "aa_hexdigest": "afd990e90f8e6db4d241d4ea86bf3ffc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12867.17819999998, "isoelectric_point": 9.774519157409667 }, "id": "JPCNHPFPBM_405", "locus": "GCA000292505_00369" }, { "type": "cds", "sequence": "contig_1", "start": 206738, "stop": 207199, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAACTATCAAGATAATGTTTTTTCCCAGGTTTTTGCTTTTAACAGGGGTAATTTAACCAACTTAAATCAACTTGATTTTTTCTATAAAGCTAACAATGCTGATAGTTACACCAAAGTAAAGGGCATTGATAGTTTAGCAAGTATGCTAGCAAACAATGGCAATGGTTTAAGCAAAGACAAAATTCTGAGTGCTTTTTTAGATCACAATTTAAACCTTTATTTAGCGAGAAGTTGGGGGGCAAATTTTGCTGGGTTTGTTGTTGATTTTAACCCAACAACCCAACTCTTTAAACTCACTCCTTTTCTAGCAAATGCTAGTTATGTTTTAACGTTAAGAGCAGTTATCTTAGCATTCTATGTCACCCAAAAGATCCTAATTATGATCTTATTTGCAACTGTACTCACTTCCACTTCAAGTTCAGTTGAACTAGCATATGGGATTGAAAGGTTATTATGA", "aa": "MFNYQDNVFSQVFAFNRGNLTNLNQLDFFYKANNADSYTKVKGIDSLASMLANNGNGLSKDKILSAFLDHNLNLYLARSWGANFAGFVVDFNPTTQLFKLTPFLANASYVLTLRAVILAFYVTQKILIMILFATVLTSTSSSVELAYGIERLL", "aa_hexdigest": "331d99e514f5958aa36d2962e941fdec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17091.436999999994, "isoelectric_point": 8.891173362731934 }, "id": "JPCNHPFPBM_406", "locus": "GCA000292505_00370" }, { "type": "cds", "sequence": "contig_1", "start": 207239, "stop": 207655, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCATTGCCATTGCCATTAGGTTTGTCCCTTCCTTGTTACTAGAATCACAACGGATCTTAAATGCCCAAGCATCCAGGGGTTTAGACTTTCGAAATGGGGGATTTTTAGTGAAGATGCGTTCACTCTCTTCGTTAGTAGTACCAATGGTCTCCATTGCATTTAGGAATGCCTCTGAACTTGCTAGTGCAATGGAAGCAAGGGGTTATCACCCTGCAAAGAAGCGCAGTAGTTATAGACAATACAAAATCACTTGGATTGATATTTTAGCGTTATTTTTGGTTTTTGCTTGGTTTGTTGTGATTATCTTTTTAACTATTAGAGGAGCGGTCTTTTTGGATCTAGGTACACCAGAATGGTTACTAACAGGAAAGATTAATGAACAGGTAATCAGGGATCTGAAGGTAAGTGGCTAG", "aa": "MTIAIAIRFVPSLLLESQRILNAQASRGLDFRNGGFLVKMRSLSSLVVPMVSIAFRNASELASAMEARGYHPAKKRSSYRQYKITWIDILALFLVFAWFVVIIFLTIRGAVFLDLGTPEWLLTGKINEQVIRDLKVSG", "aa_hexdigest": "53a7f7fc7624b89475d6c42af38c4bae", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15532.232999999984, "isoelectric_point": 10.462010002136228 }, "id": "JPCNHPFPBM_407", "locus": "GCA000292505_00371" }, { "type": "cds", "sequence": "contig_1", "start": 207738, "stop": 208382, "strand": "+", "frame": 3, "gene": "truA", "product": "tRNA pseudouridine(38-40) synthase TruA", "db_xrefs": [ "COG:COG0101", "COG:J", "EC:5.4.99.12", "GO:0001522", "GO:0003723", "GO:0009451", "GO:0009982", "GO:0031119", "GO:0160147", "SO:0001217", "UniRef:UniRef50_P47428", "UniRef:UniRef90_P47428" ], "nt": "TTGGAACAAAGTTTTTCATTAATCATTGGCAGAAAGATAAAGGTGATTGGTTCAGGTAGAACTGATAAAGGGGTACATGCCATCAACCAAACCTTTCATGTTGATATTAATGGTGAAATTAATCTCAATTTGTTAATTAGAAAAATTAACCAGTTGATTAAGCCCCACTGTATAGTTAAAACCTTGGTATTGGTTAACGATAGCTTTCATGCGCGGTTTCAAGTTAAAACCAAGGTGTATGAATATCTGATTAACTGTGGGAATTTAAACCCGTTGCAATTTAACTATGTTTGGCAGTTAAACCAGCAATTGGATCTTGAAAAACTCAAAGCTGATGCCACTTTATTTTTAGGTAAGAAAAACTTTCTTAGCTTCAGTAGTTCGATTCACACTGATTCAATTCGCACAATTAGTAAAATTACCATACAAAAAGAAACTAACCAACTAGTTAGACTAACTTTTTTTGGCAGTGGTTTTCTCAGGAGTCAAGTGAGGATGATAGTTGCTTGTTTAGTGAATTTAAACACTAATAAAATGGCACTTGAAACAGTTGCAAAATTGTTTGAACACCCCAAGAAAGGGAGTTGTGTTGTTAAAGCCCCTAGTTGTGGTTTGTATCTGAAAACAGTGGTATATGAAAAATAG", "aa": "MEQSFSLIIGRKIKVIGSGRTDKGVHAINQTFHVDINGEINLNLLIRKINQLIKPHCIVKTLVLVNDSFHARFQVKTKVYEYLINCGNLNPLQFNYVWQLNQQLDLEKLKADATLFLGKKNFLSFSSSIHTDSIRTISKITIQKETNQLVRLTFFGSGFLRSQVRMIVACLVNLNTNKMALETVAKLFEHPKKGSCVVKAPSCGLYLKTVVYEK", "aa_hexdigest": "746a0dd3964a5b1fb4c3cce16223b794", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47428", "query_cov": 1.0, "subject_cov": 0.8770491803278688, "identity": 0.995, "score": 422.0, "evalue": 6.17e-149, "valid": true, "gene": "truA", "product": "tRNA pseudouridine(38-40) synthase TruA", "ec_ids": [ "5.4.99.12" ], "uniref50_id": "UniRef50_P47428", "cog_id": "COG0101", "cog_category": "J", "go_ids": [ "GO:0001522", "GO:0003723", "GO:0009451", "GO:0009982", "GO:0031119", "GO:0160147" ] }, "pscc": { "uniref50_id": "UniRef50_P47428", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47428" ], "product": "tRNA pseudouridine synthase A" }, "genes": [ "truA" ], "id": "JPCNHPFPBM_408", "locus": "GCA000292505_00372" }, { "type": "cds", "sequence": "contig_1", "start": 208372, "stop": 209016, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAGTTACAAGTGGGATCTATCAGTTTTATTAAACAACCAAAGCTTACAAGCAAATTTTTTAAAAATTCAAACAGTTAGTGAAGCGTTAATTAAAGCTTATAACAACGGGTTGTGTTTTACAAATAAAACTAGCTTTGAACAGTTTTTAGCAATCGATGATAAGTTCACTGAACTTGAAAATCGTTACACTAACTACCTTTATAACAAGCAGAATGAAAATAACTTGGATAAGGAGGTTAATGATGCAATTTTTGCATACCAGAGTTTTAAAAATAACCATAACCTTGCTTTCAGTACACTGCAACAGGAGTTATATAACCATGAAAAACTCATTAAGGATTATTTAACTGATCCAAAGCTAGCGGTTTACAAGCGCAACTTAATGTTAGTTTTTCGCGATAAACCCCACCAACTATCTAGTCAAACCCAGAGTTTATTGAGTCAAATTAACCCTTGTTTTAACCAAGCAGAACGGATTTTTAACATCCTTTCAACTGCTGATTTAAACTTGCAACCTGTTGTTTATCAAAACAAAAAATATCCGATTAACAGTGTGAGTGATTATCAGTCCTTACTTGAAAACACTAACAGGGGGATTAGAAAAGCTTGTTATGAGAAGTGGATTGAAATTTATTGA", "aa": "MKNSYKWDLSVLLNNQSLQANFLKIQTVSEALIKAYNNGLCFTNKTSFEQFLAIDDKFTELENRYTNYLYNKQNENNLDKEVNDAIFAYQSFKNNHNLAFSTLQQELYNHEKLIKDYLTDPKLAVYKRNLMLVFRDKPHQLSSQTQSLLSQINPCFNQAERIFNILSTADLNLQPVVYQNKKYPINSVSDYQSLLENTNRGIRKACYEKWIEIY", "aa_hexdigest": "d2d2a3282c9a19d3b832092f49baa0e4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25257.233700000015, "isoelectric_point": 8.274855613708496 }, "id": "JPCNHPFPBM_409", "locus": "GCA000292505_00373" }, { "type": "cds", "sequence": "contig_1", "start": 209047, "stop": 209403, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAAAATTACATCCAACTAGAGAACTTCGCTAAACTAAAGAACCATCCTAGTTACATTGCCCAAACTGCTTTTAATGATGAGATTGAAGTTGGTTTTATTGATTTTGTTTACCAACAAGTAGCTCAGTTTGCCAAAACCTTTCAAGCATTTATTCGCTTAAAAAAACAGATTTATAAACATGTTTTAAAAGTCAATAAAGTTGAACCATATGATCTTACCCTAACACTTTTTAAAACTAAAAAACCATACACGATTGAACAAGCTAAACAAGATGCACTAAAAGTTTTAGATCTACTTGGTGACAACTACATCAAGATAGTTAAAAAGGCTTTTAATGAAAACTGGATTGATTGA", "aa": "MENYIQLENFAKLKNHPSYIAQTAFNDEIEVGFIDFVYQQVAQFAKTFQAFIRLKKQIYKHVLKVNKVEPYDLTLTLFKTKKPYTIEQAKQDALKVLDLLGDNYIKIVKKAFNENWID", "aa_hexdigest": "4b9f6bb5dd4bb3c4504aef4959db2cf0", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13967.019899999981, "isoelectric_point": 8.981558036804202 }, "id": "JPCNHPFPBM_410", "locus": "GCA000292505_00374" }, { "type": "cds", "sequence": "contig_1", "start": 209479, "stop": 210123, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACTTTGATAAAACCAAATCATCACTAAATACGTTGGTACATGAACTTGGTCATTCAGTTCACTCTTGGTATGCATCACAACACCAATCCCAAAACATCGATCCTACTATCTTTTATGCTGAGATTGCTTCTATTGCCAATGAACTGTTGTTGTGTTATTATGAACTGCAACTTTATAAAAATAACCACAAGCAGTTAATTGCTAGTTTATTGAGTCAAATTAACCATTTTTTTGGCGCTACTACAAGACAAATAATGTTTTCACAATTTGAAAAAGATACGCTTGATTTAATCAGAGTTAACCAGAAACCTGACTTTAAAACTTTGATTAAAATTTATGCAAATACTGCGGTTAAATACCAAGGTTTTAAACCTGAAGTAGTTGCTAATAAACTAAAAAAGACCCAGTATCAAAAATCATTGTCACACATCATTGCTATCCCCCATTTTTATGCAGGTAACTTCTATGTTTACAAGTATGCCATTGGCCAAGTTGCAGGTATTTTAGTAGCTAAAAAAATTAATAGTGGTGATAAAAAGATGAAAGATAATTACTTTAAATTCCTCAGTTCAGGTTCTAGTTTAGCGCCACTTGAAACCATTAAACTCTTAGGGATTGACCTTACTTCACCCCAAGCCTGA", "aa": "MNFDKTKSSLNTLVHELGHSVHSWYASQHQSQNIDPTIFYAEIASIANELLLCYYELQLYKNNHKQLIASLLSQINHFFGATTRQIMFSQFEKDTLDLIRVNQKPDFKTLIKIYANTAVKYQGFKPEVVANKLKKTQYQKSLSHIIAIPHFYAGNFYVYKYAIGQVAGILVAKKINSGDKKMKDNYFKFLSSGSSLAPLETIKLLGIDLTSPQA", "aa_hexdigest": "9dd611ecd3a63b30544fa012b5a8a63b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24363.783699999996, "isoelectric_point": 9.419169425964355 }, "id": "JPCNHPFPBM_411", "locus": "GCA000292505_00375" }, { "type": "cds", "sequence": "contig_1", "start": 210196, "stop": 210945, "strand": "+", "frame": 1, "gene": null, "product": "Type II methyltransferase M-MgeORF184P", "db_xrefs": [ "EC:2.1.1.72", "GO:0003677", "GO:0009007", "GO:0032259", "SO:0001217", "UniRef:UniRef50_Q50290", "UniRef:UniRef90_Q49400" ], "nt": "GTGCACCATTTTAACCGCGCTAAAAAGGCCAAGAATAACGAGTTTTTTACTTTAATTGATGAGATTGAAAACGAAGTAATTAACTACCAAAAGCAGTTTGCAAATAAAACCATTTTTTGTAACTGTAATGATGGTAAAAATTCCCATTTTTTTCAGTTTTTTCAAACTAACTTTAACCAGTTACAACTAAAAAAACTCATTGGGTTTAGTTTTAATAATCTCTCACAAGCTGACAAGTTCACTTTTGATGGAAATAAAGTAACTAAAACCAAATTAAAGGGTAATGGTGATTTTAGTTCTGATGAATCGATTGAAGTGTTAAAACAAGCAGATATAGTTGTAACAAATCCACCCTTTAGTTTGTTTCAAAGTTTCATTGATCTGTTAATACAACACAACAAGCAGTTTCTGGTTTTAGGGTTAAATGCAGCAGTTAGCTATAACCATATTTTTACCTACTTTAAAACTAACAAGTTGTGGTTTGGCTATACCGTTAATAAAACAATGAGTTTTTCAGTTAACAGTGACTATCAACTTTATAACCCCAAAACTAGTAACTTTTTTACAAAAAATGGCAAGTGTTTCCAAAAGATAGCAGGTATCTCTTGGTTTACTAATTTAGGAAAACCACATTATAACCCCTTTTTAAATACCAACTGTTTTTATAAAAACAACGAAAAAAACTATCCCAAGTTTGATTGGTATGATGCTATTTATGTCAACAAGATTAAAAACATCCCTATGGATTGA", "aa": "MHHFNRAKKAKNNEFFTLIDEIENEVINYQKQFANKTIFCNCNDGKNSHFFQFFQTNFNQLQLKKLIGFSFNNLSQADKFTFDGNKVTKTKLKGNGDFSSDESIEVLKQADIVVTNPPFSLFQSFIDLLIQHNKQFLVLGLNAAVSYNHIFTYFKTNKLWFGYTVNKTMSFSVNSDYQLYNPKTSNFFTKNGKCFQKIAGISWFTNLGKPHYNPFLNTNCFYKNNEKNYPKFDWYDAIYVNKIKNIPMD", "aa_hexdigest": "cddcbbd39b52f9ffb7733348c1fcaccb", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 210196, "stop": 211149, "observations": { "mutation": [ 210943 ], "stop": [ 210943 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49400", "query_cov": 1.0, "subject_cov": 0.7854889589905363, "identity": 1.0, "score": 513.0, "evalue": 2.74e-183, "gene_start": 1, "gene_end": 249, "reference_start": 1, "reference_end": 249, "reference_sequence": "MHHFNRAKKAKNNEFFTLIDEIENEVINYQKQFANKTIFCNCNDGKNSHFFQFFQTNFNQLQLKKLIGFSFNNLSQADKFTFDGNKVTKTKLKGNGDFSSDESIEVLKQADIVVTNPPFSLFQSFIDLLIQHNKQFLVLGLNAAVSYNHIFTYFKTNKLWFGYTVNKTMSFSVNSDYQLYNPKTSNFFTKNGKCFQKIAGISWFTNLGKPHYNPFLNTNCFYKNNEKNYPKFDWYDAIYVNKIKNIPMDWNGLMGVPLTFLNCYNPKQFELVDCLANPYATLDTLKTNAFVKLNQGDVRNVNGKRRYVRVIIKKQQI" }, "paralog": false, "identity": 0.9936908517350158, "score": 658.0, "evalue": 7.04e-243, "description": "Internal stop codon at 210943. Nonsense mutation around 210943.", "psc": { "uniref90_id": "UniRef90_Q49400", "product": "Type II methyltransferase M.MgeORF184P", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_Q50290", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0032259" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49400", "EC:2.1.1.72", "UniRef:UniRef50_Q50290", "GO:0003677", "GO:0009007", "GO:0032259" ] }, "pscc": { "uniref50_id": "UniRef50_Q50290", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50290" ], "product": "Type II methyltransferase M.MpnI" } }, "id": "JPCNHPFPBM_412", "locus": "GCA000292505_00376" }, { "type": "cds", "sequence": "contig_1", "start": 210955, "stop": 211149, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGTTCCTTTAACCTTTTTAAACTGTTACAACCCCAAGCAGTTTGAATTAGTTGATTGTCTTGCTAACCCTTATGCTACCTTAGATACATTAAAAACAAATGCCTTTGTGAAATTAAATCAGGGTGATGTGAGAAATGTTAATGGTAAAAGAAGGTATGTAAGGGTAATAATTAAAAAACAACAAATTTAG", "aa": "MGVPLTFLNCYNPKQFELVDCLANPYATLDTLKTNAFVKLNQGDVRNVNGKRRYVRVIIKKQQI", "aa_hexdigest": "19e9acc1ba819a4c2e570ba6152ca2bd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7371.567700000002, "isoelectric_point": 9.757757377624511 }, "id": "JPCNHPFPBM_413", "locus": "GCA000292505_00377" }, { "type": "cds", "sequence": "contig_1", "start": 211249, "stop": 211875, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTCAAACTATTTTTTTTATCAAGCAGTGTGTTGGGTCCAATTGCCCTATTTACAACTGCTTGTAGTGCTGTTTATAGGTTTGATCAAGTTGATGATGGCAAGATTAAACTAGCAACTGTAACTTCAGCTTCCGCTAGTGGCTCGCTTACTACTATCATCAGTAAATATAATTCACAAAAAGATCCTAATGATTATCCAGTGGAACTGGTTTCACTTGATAGTAGGGGCAGTTATTCTAATGGCAAAAAGGATCTGCAAGCTAAACTGCTAGCTAAGGATAAAAATAACTTTTATAACCTTACTTTTAACTATAGTGATGTAGTTTCAATCCTCTCAAGAAGCCAGATGGAGTTGAGCTTTGATACGGTTGATACTAGTAATTTTGATCCTAGTTTTCTTAGTTTTAACAATAACATTTCCAATGTTAATCCAAATAGCATCTATGCTTTACCTGCTACTGTATCAGGTGAAGTTTTAGTTTTAAATGGACCGGTGTTACATTACATTTTAAGTAGTGCTAAAAAAGATAGTAACACCACCCTTTCAACCCATTCAGCTAGCAATAATAGCAATAAGGGAACAATGGTTGTTGCTAGTGATAGTGAAACATCAAGTTTATGA", "aa": "MKFKLFFLSSSVLGPIALFTTACSAVYRFDQVDDGKIKLATVTSASASGSLTTIISKYNSQKDPNDYPVELVSLDSRGSYSNGKKDLQAKLLAKDKNNFYNLTFNYSDVVSILSRSQMELSFDTVDTSNFDPSFLSFNNNISNVNPNSIYALPATVSGEVLVLNGPVLHYILSSAKKDSNTTLSTHSASNNSNKGTMVVASDSETSSL", "aa_hexdigest": "45ade32ed9956687bfb8a4a4a7b370fd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22476.832100000014, "isoelectric_point": 6.079066658020018 }, "id": "JPCNHPFPBM_414", "locus": "GCA000292505_00378" }, { "type": "cds", "sequence": "contig_1", "start": 211900, "stop": 212064, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCTCAAACTAATGAAACCCAAGTTTTAAAAAGTAATTCATCAGAATCTAACCAAACCCAAGCTAGTGATACAGAGATTAAAAAGATTTGGGGTGATTATCAAGAAGTTGATGGAGGGTTGAAAAATTACACCTTTAAAGCTAGTGTTTTTAACAATTGA", "aa": "MNAQTNETQVLKSNSSESNQTQASDTEIKKIWGDYQEVDGGLKNYTFKASVFNN", "aa_hexdigest": "2a354eb9ad66692db4e9e73bce5190c4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6060.453400000002, "isoelectric_point": 4.715101432800293 }, "id": "JPCNHPFPBM_415", "locus": "GCA000292505_00379" }, { "type": "cds", "sequence": "contig_1", "start": 212170, "stop": 213354, "strand": "+", "frame": 1, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0005886", "GO:0009897", "SO:0001217", "UniRef:UniRef50_Q50289", "UniRef:UniRef90_P47431" ], "nt": "TTGGAAAATGCCTTATATACTGCTTTATTTGCTTCTGGAAAAGCTGATTACAATAACTTTCTTTTTAACATCAAAAACCAGCGAATTAATTTCAGTAACTTTTTTAATAAATCCTCAACTGCATTTCAAAACCTTAAAACTATCTTTAACAGCTTCAAATCCTTAATTGATCAAAACGGTTTAATCTCAAATGCACACTTTAACACCCCAGTTAATGACTATGCTAAGTTTAACCAATTAGCTTTTTACACCTCTTCAACTGCACGCTTTCCCTATTCATTTGCAAGTGATAGTGTAAAGCGTTTAATAGTTAATGACAAGACAATTGAAAACAAAAACAATAAGAGTGTTTTTGAGGTTAATTTAAGTAGTGATAGTGATAACAACAGTAACCTAATTGGTACTGTATCACTGGAAAATAGTAAACAAGTTTCACTCTATGAAAAGCAAGTGGATAGTAATAAACAAATTGGTGTTGATGCTTTGTTAATCAAAGATGAAACTTTAATCAACCATCTTAAGAGTTTAAAATCGCAAGTTAGTGCAAAAAGTGCTAGTGAAACTTCCCAAACAAAACAAAACAAAACCTTTTTAGCATTTACAACTGTGAATGCAGATCAAAAAGCAATCTTTGATGTTGGTAAACTTAATGGCAAAACTGCCAAAATCATTATTAATGCTACTGAAACAACTAATGCTAAGATTAGCACCTTACAAGAAAAAGAGGCAATAGTTCTAAAAGCACCCCAACGCTTTGAGAGCACTGATCCATTTCCTATTGCTTTAGTGCAAGGTCCTAGTTTAATAGGGATCCATGCAAATGAAAGAGAGGATATTGAAACCAAAAAGTTTGTGAATTGGTATCTGAATACTAAAGTCCAATGGAAAGAAAATTCTATTAAAACTCCTGCAGAATATGTAGCTGATAAAGCTTCTTATCTTTTACCTTTTAAAAATAGGCTAAATAATACTAACAGTTACAATGAGTTTGTTAAAACTGCAGTTAGTCAGTTTGCTGATAAAAATGTAACTAAATTTGCAGAACCTGCTGATTTTTTATCCAACAAAGTACGTGATGGTGTTAAGAGTAATTTAAATGCTGCAATTAACAACCACAGCATTGATTTTGATAGTTTTATCAATGATTTAACAGACTATCTTGGTAGTGATGTTAAAAACATCTAA", "aa": "MENALYTALFASGKADYNNFLFNIKNQRINFSNFFNKSSTAFQNLKTIFNSFKSLIDQNGLISNAHFNTPVNDYAKFNQLAFYTSSTARFPYSFASDSVKRLIVNDKTIENKNNKSVFEVNLSSDSDNNSNLIGTVSLENSKQVSLYEKQVDSNKQIGVDALLIKDETLINHLKSLKSQVSAKSASETSQTKQNKTFLAFTTVNADQKAIFDVGKLNGKTAKIIINATETTNAKISTLQEKEAIVLKAPQRFESTDPFPIALVQGPSLIGIHANEREDIETKKFVNWYLNTKVQWKENSIKTPAEYVADKASYLLPFKNRLNNTNSYNEFVKTAVSQFADKNVTKFAEPADFLSNKVRDGVKSNLNAAINNHSIDFDSFINDLTDYLGSDVKNI", "aa_hexdigest": "8eb88c99db47b681ef3356bf88a8aea6", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 211876, "stop": 213354, "observations": { "mutation": [ 212062 ], "stop": [ 212062 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47431", "query_cov": 1.0, "subject_cov": 0.5620542082738944, "identity": 0.995, "score": 710.0, "evalue": 2.1e-252, "gene_start": 1, "gene_end": 394, "reference_start": 308, "reference_end": 701, "reference_sequence": "MKFKLFFLSSSVLGPIALFTTACSAVYRFDQVDDGKIKLATVTSASASGSLTTIISKYNSQKDPNDYPVELVSLDSRGSYSNGKKDLQAKLLAKDKNNFYNLTFNYSDVVSILSRSQMELSFDTVDTSNFDPSFLSFNNNISNVNPNSIYALPATVSGEVLVLNGPVLHYILSSAKKDSNTTLSTHSASNNSNKGTMVVASDSETSSLWTKLEAAAKMNAQTNETQVLKSNSSESNQTQASDTEIKKIWGDYQEVDGGLKNYTFKASVFNNWKDLNDFATRIAKSFTKLQTTTKKGEEVQAVFGIGSLENALYTALFASGKADYNNFLFNIKNQRINFSNFFNKSSTAFQNLKTIFNSFKSLIDQNGLISNAHFNTPVNDYAKFNQLAFYTSSTARFPYSFASDSVKRLIVNDKTIENKNNKSVFEVNLSSDSDNNSNLIGTVSLENSKQVSLYEKQVDSNKQIGVDALLIKDETLINHLKSLKSQVSAKSASETSQTKQNKTFLAFTTVNADQKAIFDVGKLNGKTAKIIINATETTNAKISTLQEKEAIVLKAPQRFESTDPFPIALVQGPSLIGIHANEREDIETKKFVNWYLNTKVQWEENSIKTPAEYVADKASYLLPFKNRLNNTNSYNEFVKTAVSQFADKNVTKFAEPADFLSNKVRDGVKSNLNAAINNHSIDFDSFINDLTDYLGSDVKNI" }, "paralog": false, "identity": 0.9451219512195121, "score": 860.0, "evalue": 7.48e-314, "description": "Internal stop codon at 212062. Nonsense mutation around 212062.", "psc": { "uniref90_id": "UniRef90_P47431", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_Q50289", "go_ids": [ "GO:0005886", "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47431", "UniRef:UniRef50_Q50289", "GO:0005886", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_Q50289", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50289" ], "product": "Uncharacterized lipoprotein MPN_199" } }, "id": "JPCNHPFPBM_416", "locus": "GCA000292505_00380" }, { "type": "cds", "sequence": "contig_1", "start": 213381, "stop": 213740, "strand": "+", "frame": 3, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7M3VDH2", "UniRef:UniRef90_UPI001EE65182" ], "nt": "GTGTTACTAGTGCCTAAGGTGGTAGTGGAGTACCACCAACTTGATAAGAAGGTTGTCAAAGAGAGTTTGGAAGTGGAAGCAACTTCCACCTTCAACCCCACCCAAAGGTTGAAGAGTGATAGTCCAATGAAGGATTCAACAAAAACAGGGGAGAAACTCCAAGAGACCATGTCATCAATGAGTGGTATGGCTACATCCACTCGCAAGGCCCTCAAAGTGGAGGTGGAAAGGGGGAGTAATGTCAATCAAGGCGAACTAGCAAAAAACGACTTTGCTAAAAAGCCACTAAAGCATAAGAACAGTAGTGGGACAGATGTGAAGTTAGATTCACAGAAGGATTTTACTGAAGGAAAGGTTTGA", "aa": "MLLVPKVVVEYHQLDKKVVKESLEVEATSTFNPTQRLKSDSPMKDSTKTGEKLQETMSSMSGMATSTRKALKVEVERGSNVNQGELAKNDFAKKPLKHKNSSGTDVKLDSQKDFTEGKV", "aa_hexdigest": "2f6e9dab076ed1f29f6b0335bb7150a8", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030D24C90", "query_cov": 0.9243697478991597, "subject_cov": 0.8661417322834646, "identity": 0.764, "score": 149.0, "evalue": 1.84e-44, "valid": false, "product": "adhesin", "uniref50_id": "UniRef50_A0A7M3VDG5" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 213390, "stop": 213896, "observations": { "mutation": [ 213738, 213879 ], "stop": [ 213738, 213879 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001EE65182", "query_cov": 0.9663865546218487, "subject_cov": 0.6845238095238095, "identity": 1.0, "score": 210.0, "evalue": 5.97e-68, "gene_start": 5, "gene_end": 119, "reference_start": 2, "reference_end": 116, "reference_sequence": "MPKVVVEYHQLDKKVVKESLEVEATSTFNPTQRLKSDSPMKDSTKTGEKLQETMSSMSGMATSTRKALKVEVERGSNVNQGELAKNDFAKKPLKHKNSSGTDVKLDSQKDFTEGKVWKPVLTTDEIKKNRGMGAFANSPSPSTSASSLPHSPLFLTSMLGLNQWSLNT" }, "paralog": false, "identity": 0.9821428571428571, "score": 320.0, "evalue": 9.54e-114, "description": "Internal stop codon at 213738, 213879. Nonsense mutation around 213738, 213879.", "psc": { "uniref90_id": "UniRef90_UPI001EE65182", "product": "adhesin", "uniref50_id": "UniRef50_A0A7M3VDH2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001EE65182", "UniRef:UniRef50_A0A7M3VDH2" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7M3VDH2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7M3VDH2" ], "product": "MgpB adhesin protein" } }, "id": "JPCNHPFPBM_417", "locus": "GCA000292505_00381" }, { "type": "cds", "sequence": "contig_1", "start": 214007, "stop": 214372, "strand": "+", "frame": 2, "gene": null, "product": "Random genomic clone esh10, partial cds", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49206" ], "nt": "GTGAAGCCTAGCAATAGTAGTAGTAGCAACACGTTTAATCCCAATGATGATGAAAATCAAGTCACTCAAGGTGGCTCCTCCAAAAAAACAACGTATGACAATTTACCAAACTCCATCAGTCCCACCAGTGACTGGATCAACGCATTAACCTTCACCAATAAGAACAATCCACAACGCAATCAGTTGTTGCTCAGAAGCTTACTAGGAACGATCCCGGTATTGATCAATAAGAGTGGGGAGGGTGGGAATGAGTTTAACCATACGAATGATCAGAAGTGGGATAAAACGAATGAAAAAGATGGGAATTTACCTGGGTTTGGGGAGGTGAATGGTGGTTTTATTAAGTTTTTATCAAATTTTTATTAG", "aa": "MKPSNSSSSNTFNPNDDENQVTQGGSSKKTTYDNLPNSISPTSDWINALTFTNKNNPQRNQLLLRSLLGTIPVLINKSGEGGNEFNHTNDQKWDKTNEKDGNLPGFGEVNGGFIKFLSNFY", "aa_hexdigest": "1cf5c25e5563fafd5a3f0c4dafdb513b", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030D20171", "query_cov": 0.9669421487603306, "subject_cov": 0.9915254237288136, "identity": 0.795, "score": 181.0, "evalue": 6.9e-57, "valid": false, "product": "adhesin", "uniref50_id": "UniRef50_Q49206" }, "pscc": { "uniref50_id": "UniRef50_Q49206", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49206" ], "product": "Random genomic clone esh10, partial cds" }, "genes": [], "id": "JPCNHPFPBM_418", "locus": "GCA000292505_00382" }, { "type": "cds", "sequence": "contig_1", "start": 214768, "stop": 214938, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13", "UniRef:UniRef90_UPI002FCE54A6" ], "nt": "GTGGTGAAAAAATACCAGTTGGATGTACCGAATGTTACCAATGAGATGAACGATGTACTGAAAGAGTTGATCCTTGAACAACCTTTGACTAAATATACCTTAAATTCGAGTTTGGCTAAACAGAAGGGTAAGACACAAGCAGTGGTGCATCTCTCAAATTCAAATCAGTGA", "aa": "MVKKYQLDVPNVTNEMNDVLKELILEQPLTKYTLNSSLAKQKGKTQAVVHLSNSNQ", "aa_hexdigest": "497f29254cf1771f06d89844e8a0e773", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 214626, "stop": 215003, "observations": { "insertion": [ 214662 ], "deletion": [ 214954 ], "mutation": [ 214960 ], "stop": [ 214762, 214936, 214960 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FCE54A6", "query_cov": 0.9107142857142857, "subject_cov": 0.4112903225806452, "identity": 0.863, "score": 89.0, "evalue": 1.39e-21, "gene_start": 1, "gene_end": 51, "reference_start": 47, "reference_end": 97, "reference_sequence": "MLFKSTQVKLTYSSSGSNNISFENNANKPSYVVEFTNSTTVGIKWSVVKKYQLDVPNVTNEMNQVLQELILEQPLTKYTLNGSLAKQKGKSQVAVHLGGQASQWHSTRHSQSKQQSQPQGFNRI" }, "paralog": false, "identity": 0.746031746031746, "score": 159.0, "evalue": 5.98e-52, "description": "Internal stop codon at 214762, 214936, 214960. Frameshift due to insertion around 214662. Frameshift due to deletion around 214954. Nonsense mutation around 214960.", "psc": { "uniref90_id": "UniRef90_UPI002FCE54A6", "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FCE54A6", "UniRef:UniRef50_UPI00027B3D13" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6343.244200000003, "isoelectric_point": 9.10114688873291 }, "id": "JPCNHPFPBM_419", "locus": "GCA000292505_00383" }, { "type": "cds", "sequence": "contig_1", "start": 215066, "stop": 215218, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGACCAAGCAGGGCAAAGGGAAGGATAGTAGTGGGTGGAGTTCAACTGAAGAAACAACGGCAGCAGGGGATGCGCCCAGTGTTACTGGAGGGGGAACATCAGACACCGCTTCAAAATTTAAAAGTTACCTCAACACCAAACAAGCGTTAG", "aa": "MGPSRAKGRIVVGGVQLKKQRQQGMRPVLLEGEHQTPLQNLKVTSTPNKR", "aa_hexdigest": "b9391b1c63cc47074939f28d320178a0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5548.458400000001, "isoelectric_point": 11.849047279357908 }, "id": "JPCNHPFPBM_420", "locus": "GCA000292505_00384" }, { "type": "cds", "sequence": "contig_1", "start": 215258, "stop": 215557, "strand": "+", "frame": 2, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FCAC30C" ], "nt": "ATGTGGTTACCCTCCTTCCTCTACTTTCAACCCAACAAGGTGAAAAGTGGTCAATATCAACAAAATAACACCTACAACAGGTTAATTGAACCGGAAAGTGCAACAAGTGCAGCAACTAACATGACCAACTTGTTAAAGCTGTTGTCTAGTAAAAACATCAAACAAAAGTTGGGGAAGGGGGACACCCAATCAATGGGAAATAATGGAGTGTCCAAGACACCTTCTCATTTGTTGTTCCTTATTCGGGGAATCATGACAATAATGGAACAACTGGACCCATTAAAACTGCTTATCCAGTGA", "aa": "MWLPSFLYFQPNKVKSGQYQQNNTYNRLIEPESATSAATNMTNLLKLLSSKNIKQKLGKGDTQSMGNNGVSKTPSHLLFLIRGIMTIMEQLDPLKLLIQ", "aa_hexdigest": "f6b71c0df9282b5e442263d3691980f4", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00027B3797", "query_cov": 0.98989898989899, "subject_cov": 0.8990825688073395, "identity": 0.7240000000000001, "score": 124.0, "evalue": 5.36e-35, "valid": false, "uniref50_id": "UniRef50_UPI001FCAC30C" }, "pscc": { "uniref50_id": "UniRef50_UPI001FCAC30C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FCAC30C" ], "product": "adhesin" }, "genes": [], "id": "JPCNHPFPBM_421", "locus": "GCA000292505_00385" }, { "type": "cds", "sequence": "contig_1", "start": 215814, "stop": 215972, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGGGTTTTTAAAACCAAATTTCTCGCTCGGTGCTTTGTTTTTAACTTTAAGCCCCATAGCCACTGCATGCATTGCTGAAAAACCAGTTAACAACCGCTTTAACTTTAATAGCGAGCAATTAGCTAGGCTAAGAAAAGCAAGGGTTAACCACTGA", "aa": "MKGFLKPNFSLGALFLTLSPIATACIAEKPVNNRFNFNSEQLARLRKARVNH", "aa_hexdigest": "ba96a79688dc3cbf84dc2154b7a16318", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5845.803, "isoelectric_point": 11.006509971618652 }, "id": "JPCNHPFPBM_422", "locus": "GCA000292505_00386" }, { "type": "cds", "sequence": "contig_1", "start": 215988, "stop": 216164, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAGTTAGCTTTGCAAATAACCACCAAAAACCGATCCGTATCTATGCCATTGATACCCCTGAAAAAGCAGTTTTATCTATAAAACGCAAATCAGAGATAGAACTTAAAGAAGCTAATAAAGCAACTGAGTTTGCCAAAAGCTTAATTCCCATTGGTAGTGAGGTGTGGATCTGA", "aa": "MEVSFANNHQKPIRIYAIDTPEKAVLSIKRKSEIELKEANKATEFAKSLIPIGSEVWI", "aa_hexdigest": "acbcbe91ad10a9f7a5bfcf96a36c509c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6583.566399999998, "isoelectric_point": 8.0579195022583 }, "id": "JPCNHPFPBM_423", "locus": "GCA000292505_00387" }, { "type": "cds", "sequence": "contig_1", "start": 216204, "stop": 216476, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B418B8", "UniRef:UniRef90_UPI0001B418B8" ], "nt": "GTGTTTTTCAAAACCAATCCATTGCAACTGCACTTTGAATCGTTTGCAGTTGAAATGGTAGCAAATGGTCATGCTTTACCTATTGCTGGTAATGACTTTGATTTTGTTTTTAGTGATTTGGACCCATTTAATCCCCTAAAAATAGTAGGGATTGAACTAGCTAATGGTTTAAACAATGCTTTTAACAACAGAAAAAACATCTTTAGTTATTTAGAAAACAGTTTTCAATCAATAACAATGGTCTATCAACAACGCGGTGTTGACCAAAGTTGA", "aa": "MFFKTNPLQLHFESFAVEMVANGHALPIAGNDFDFVFSDLDPFNPLKIVGIELANGLNNAFNNRKNIFSYLENSFQSITMVYQQRGVDQS", "aa_hexdigest": "240d5fe9c49f5fc1a8f13eb332e8c5a7", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 216153, "stop": 216566, "observations": { "mutation": [ 216162, 216474 ], "stop": [ 216162, 216474 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001B418B8", "query_cov": 1.0, "subject_cov": 0.656934306569343, "identity": 0.9890000000000001, "score": 179.0, "evalue": 1.02e-56, "gene_start": 1, "gene_end": 90, "reference_start": 18, "reference_end": 107, "reference_sequence": "MWIWPLNSYSYDREVAAVFFKTNPLQLHFESFAVEMVANGHALPIAGNDFDFVFSDLDPFNPLKIVGIELANGLNNAFNNRKNIFSYLENSFQSITMVYQQRGVDQSWTRYLAPSNDFSSTKLGLGLTIYELKLNNG" }, "paralog": false, "identity": 0.9781021897810219, "score": 270.0, "evalue": 4.63e-95, "description": "Internal stop codon at 216162, 216474. Nonsense mutation around 216162, 216474.", "psc": { "uniref90_id": "UniRef90_UPI0001B418B8", "uniref50_id": "UniRef50_UPI0001B418B8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001B418B8", "UniRef:UniRef50_UPI0001B418B8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001B418B8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B418B8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10206.387099999996, "isoelectric_point": 4.7775674819946286 }, "id": "JPCNHPFPBM_424", "locus": "GCA000292505_00388" }, { "type": "cds", "sequence": "contig_1", "start": 216559, "stop": 216888, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33", "PFAM:PF03215.21" ], "nt": "ATGGCTAACAATAAGAGTGCAATTGAGTTGAAAAACATCGTTGTTGATTTTGGTGAATCAGTTGCGATTGACAACATTAACCTTAGTGTTGAAAAACACCAACTAGTTAGCTTACTTGGTCCTAGTGGTTGTGGTAAAACCACTACACTTGCAGTTATTGCAGGACTTATTAAACCAACTAGTGGTCAGGTGTTATTTAATGGTTATGATGTCACCAAAAAACCACCCCAAGAACGTAAACTAGGGCTAGTTTTTCAAAACTATGCACTTTATCCGCACATGAATGTGTTTGAAAACATTGTTTTCCCTCTCTACAGTGATAACTCGTGA", "aa": "MANNKSAIELKNIVVDFGESVAIDNINLSVEKHQLVSLLGPSGCGKTTTLAVIAGLIKPTSGQVLFNGYDVTKKPPQERKLGLVFQNYALYPHMNVFENIVFPLYSDNS", "aa_hexdigest": "b932d116271233ef4cdc0f6ca5d63cc5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034E961FD", "query_cov": 0.8440366972477065, "subject_cov": 0.8679245283018868, "identity": 0.5329999999999999, "score": 114.0, "evalue": 9.31e-31, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_A0A3B9G3L7" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 78, "aa_cov": 0.6972477064220184, "hmm_cov": 1.0, "evalue": 2.9314588031910714e-21, "score": 71.6120834350586, "start": 24, "stop": 99 }, { "id": "PF03215.21", "name": "Rad17", "length": 79, "aa_cov": 0.7155963302752294, "hmm_cov": 0.9873417721518988, "evalue": 4.487790562181142e-08, "score": 28.33935546875, "start": 23, "stop": 100 } ], "seq_stats": { "molecular_weight": 11895.559399999991, "isoelectric_point": 6.710377311706543 }, "id": "JPCNHPFPBM_425", "locus": "GCA000292505_00389" }, { "type": "cds", "sequence": "contig_1", "start": 216901, "stop": 217194, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAAAAAACAGTGTTGCAAACCATGAGATTAACTGTTTGTTACTTACTAGCAACGGTGCATCAGTTCAAGAGATTGATCAGCTCAATAAGTTATTTCATGATAGTATTGAAAAACCCAAACAGATCCAATACCAAATTAATGACCTTAATGTTAGTGTTTTTAAAAACTTAAATGAACTAACTGCAAACCTTAAGTTAATACCAAGTAAGCACCAGTTTGCTATTACCAATCTCAACAAACAAACTCTAAAACAGATTAATGAACTGGAAGCTGAGTTTAAAACAAAGTGA", "aa": "MEKNSVANHEINCLLLTSNGASVQEIDQLNKLFHDSIEKPKQIQYQINDLNVSVFKNLNELTANLKLIPSKHQFAITNLNKQTLKQINELEAEFKTK", "aa_hexdigest": "df1742a48338dccf1a178445687dc124", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11119.565499999986, "isoelectric_point": 6.824282646179199 }, "id": "JPCNHPFPBM_426", "locus": "GCA000292505_00390" }, { "type": "cds", "sequence": "contig_1", "start": 217315, "stop": 218019, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "GTGGAACTAAAACAAACCCTTGTTGAAAACCTTAAGTTAGTTAAAAAAGCGATTAGTGAACAAACTAAGTTAATTAAACAGAGTAGTGATTACACTAAGTTAAAGCAATTAAAACGGTTAATTAAAGTTGAACCTAACCAACTCAAAAAACAATATAAGGTTTTTCTCAATCAGTTAATTAAAAACTATTCACTTAAAACTGATAAGTTAACTGATACTCAACTTAATGAAATTGAACAGATTAAAACCAGAATTGTTTCAATAAAACAGTTTATCAACAAAACTGCACTTGAAGTTGCTAACAAACTAGCGATTACCAAGATTTTAACCAAACGCCCTGATAAGATTTCTGGTGGACAACAACAACGCGTAGCAATTGCTAGAGCAATTGTCAGAAGACCTAAACTATTGTTAATGGATGAACCACTCTCTAACTTAGATGCAAAGCTAAGGGTACAGACAAGACAGTGGATCAGACAGTTTCAACAGGAGTTACAAATTACCACTGTTTTTGTCACCCATGACCAGGAAGAAGCGATGAGTATTAGTGATGTCATTGTTTGTATGTCAACTGGAAAAGTGCAGCAAATCGGCACACCCAGTGAACTTTATTTAAAACCTGCTAATGAGTTTGTTGCGCGCTTTTTAGGCACCCCTGAGATGAACATCATTGAATGTAGTGTCAAAAACAACCAGTTGTTTTGA", "aa": "MELKQTLVENLKLVKKAISEQTKLIKQSSDYTKLKQLKRLIKVEPNQLKKQYKVFLNQLIKNYSLKTDKLTDTQLNEIEQIKTRIVSIKQFINKTALEVANKLAITKILTKRPDKISGGQQQRVAIARAIVRRPKLLLMDEPLSNLDAKLRVQTRQWIRQFQQELQITTVFVTHDQEEAMSISDVIVCMSTGKVQQIGTPSELYLKPANEFVARFLGTPEMNIIECSVKNNQLF", "aa_hexdigest": "35f563f66be9cc9453b00407e146a237", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 143, "aa_cov": 0.5854700854700855, "hmm_cov": 0.7692307692307693, "evalue": 6.793219889186864e-14, "score": 47.75909423828125, "start": 7, "stop": 143 } ], "seq_stats": { "molecular_weight": 27008.450900000003, "isoelectric_point": 9.8450475692749 }, "id": "JPCNHPFPBM_427", "locus": "GCA000292505_00391" }, { "type": "cds", "sequence": "contig_1", "start": 218098, "stop": 218316, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTCACTACTAACAAAAGTAGTTTGCAAGCTAAACTAATTAACATTGAAAACTTAGGTAAACACTTAGTTGCTACCATTAGTTTGTTTGATACCACCTTATCAATGCGCTTAGAATTGAATAGCCACTTAAAAGTAGGTGATAGTTTAAATTTCATTATTAAAGCTAACAACCTCCATTTTTTTGATATTGATACAAAACAACGGATTGAGATTTAA", "aa": "MVTTNKSSLQAKLINIENLGKHLVATISLFDTTLSMRLELNSHLKVGDSLNFIIKANNLHFFDIDTKQRIEI", "aa_hexdigest": "e837cd6c9e16c876478aabd4345a93c9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8168.404699999995, "isoelectric_point": 8.158812522888184 }, "id": "JPCNHPFPBM_428", "locus": "GCA000292505_00392" }, { "type": "cds", "sequence": "contig_1", "start": 218412, "stop": 218693, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCCTCCCTGCGCTTTTAACAACAATTTTGTTTACCATTATCCCCTTCTTTTTAAGCTTACAGAAGGGTTTTAGTGCTAACAGTGATCTGTATGATCTCTCCTCCCAATTCTTTAGTTTACGAACCTTTCAGGATCTGTTTAGTGAATCTAACTTTGTGTTGGGCTTACGTAACAGTTTTCTCTATTCACTCATCTCTTTACCCTTTAGCATTATTATTGCTATTGTTATTGCCAGTGCCATAGTATTTGTGTACAAAAAATTGTTAAGAGGGTTTTGA", "aa": "MFLPALLTTILFTIIPFFLSLQKGFSANSDLYDLSSQFFSLRTFQDLFSESNFVLGLRNSFLYSLISLPFSIIIAIVIASAIVFVYKKLLRGF", "aa_hexdigest": "9252d7e3eba107482252cf8828c672ff", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10559.398399999987, "isoelectric_point": 9.30054759979248 }, "id": "JPCNHPFPBM_429", "locus": "GCA000292505_00393" }, { "type": "cds", "sequence": "contig_1", "start": 218700, "stop": 219263, "strand": "+", "frame": 3, "gene": "ugpA", "product": "putative ABC transporter permease protein MG188", "db_xrefs": [ "COG:COG1175", "COG:G", "GO:0005886", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P75263", "UniRef:UniRef90_P47434" ], "nt": "GTGTTTTTTTTACCCTATGTAACTTCAGGGGTGGCAATCTCGATTGCCTTTGTATATATCTTTGATTCTGCCTCTGGTATTTTAAACACGGTGTTTAATGTCAACACCAAGTGGCTTGATTCAGGTTCACGTGATACATTTAATGCCTTGTGGGCTATCTTAATCTTTGGGGTGTGGAAAAACTTGGCATTTAATGTGTTGATTATCTCAACAGCAATGTTAAGTGTTAATCCCCAACTTTACAAGGTAGCAAGTTTAGATAGTGCCAATCCTGTAAGACAGTTCTTTAAGATCACTCTTCCCTCCATCCGTCCTACTTTAATCTTTCTTACTACCCTTTTAATCTTAGGGGGGATGCAAGTCTTTCCGCTGGCTTTGTTTGAAAACAAACCTGAAGAAGCGGTTGCTAATGGGGGGAATAGTATCTTGCTTTACATCTTTCAACAGATCCAAAGTGGCAATACTAACTTAGCAGGTGCTGCTACTTTAGTGTTGTTTGTGTTGGGAGTTTGTTATGGGTTAGTGTTACGTAATGGCTTTTATCTGATTGAATGGTTGCAGTGA", "aa": "MFFLPYVTSGVAISIAFVYIFDSASGILNTVFNVNTKWLDSGSRDTFNALWAILIFGVWKNLAFNVLIISTAMLSVNPQLYKVASLDSANPVRQFFKITLPSIRPTLIFLTTLLILGGMQVFPLALFENKPEEAVANGGNSILLYIFQQIQSGNTNLAGAATLVLFVLGVCYGLVLRNGFYLIEWLQ", "aa_hexdigest": "23a383ef9e846a29711dce8de3158b3a", "start_type": "GTG", "rbs_motif": null, "genes": [ "ugpA" ], "truncated": "both", "pseudogene": { "start": 218400, "stop": 219308, "observations": { "mutation": [ 218691, 219261 ], "stop": [ 218691, 219261 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47434", "query_cov": 1.0, "subject_cov": 0.5683890577507599, "identity": 0.995, "score": 359.0, "evalue": 3.84e-123, "gene_start": 1, "gene_end": 187, "reference_start": 128, "reference_end": 314, "reference_sequence": "MFKWLLKHHNQPHSLQLGLLDQPLPFWKPFLLFLPALLTTILFTIIPFFLSLQKGFSANSDLYDLSSQSFSLRTFQDLFSESNFVLGLRNSFLYSLISLPFSIIIAIVIASAIVFVYKKLLRGFWQTVFFLPYVTSGVAISIAFVYIFDSASGILNTVFNVNTKWLDSGSRDTFNALWAILIFGVWKNLAFNVLIISTAMLSVNPQLYKVASLDSANPVRQFFKITLPSIRPTLIFLTTLLILGGMQVFPLALFENKPEEAVANGGNSILLYIFQQIQSGNTNLAGAATLVLFVLGVCYGLVLRNGFYLIEWLQWKIKQLYVQKQLTLY" }, "paralog": false, "identity": 0.9900662251655629, "score": 575.0, "evalue": 7.73e-211, "description": "Internal stop codon at 218691, 219261. Nonsense mutation around 218691, 219261.", "psc": { "uniref90_id": "UniRef90_P47434", "gene": "UgpA", "product": "Probable ABC transporter permease protein MG188", "uniref50_id": "UniRef50_P75263", "cog_id": "COG1175", "cog_category": "G", "go_ids": [ "GO:0005886", "GO:0055085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47434", "UniRef:UniRef50_P75263", "COG:COG1175", "COG:G", "GO:0005886", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P75263", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75263" ], "product": "Probable ABC transporter permease protein MG188 homolog" } }, "id": "JPCNHPFPBM_430", "locus": "GCA000292505_00394" }, { "type": "cds", "sequence": "contig_1", "start": 219808, "stop": 219963, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCCTTTGTTAGCAGTGTTTTTAGTGGGTTTATGTACCGTACTAGCTTTGAAGCCATTCCATCTCAATTAAAAGAGTCAGCACTCATTGATGGGTGTAATGGCTTTAACTACTTTTTGAAGATTGCTTTACCAATGGTGAAATCTACCAGTTGA", "aa": "MPFVSSVFSGFMYRTSFEAIPSQLKESALIDGCNGFNYFLKIALPMVKSTS", "aa_hexdigest": "085b3f2dbe63731aa5df8fccf6c2c25d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5658.5434, "isoelectric_point": 7.899262809753418 }, "id": "JPCNHPFPBM_431", "locus": "GCA000292505_00395" }, { "type": "cds", "sequence": "contig_1", "start": 220036, "stop": 220236, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATTTAAACATTAACTTGTGGGTGTTACAACAAGGGATCTTGGATGCTAACAGTAGTGATGAACAGATCAGAACGCTGTTAAATCTCAAGATGAGTGCAGCGATTCTAGCCATCCTTCCGATGTTTATTATCTACTTTTTGTTCCATAAAAGGATTATGAATGCCATTAAAAACAGAGCCAACACCATTAAGGGTTAA", "aa": "MDLNINLWVLQQGILDANSSDEQIRTLLNLKMSAAILAILPMFIIYFLFHKRIMNAIKNRANTIKG", "aa_hexdigest": "4b4661cc8f13f3dff15fd38d9d37aa1a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7572.9792999999945, "isoelectric_point": 9.817390632629394 }, "id": "JPCNHPFPBM_432", "locus": "GCA000292505_00396" }, { "type": "cds", "sequence": "contig_1", "start": 220238, "stop": 220393, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_158309334.1", "SO:0001217", "UniParc:UPI0002F596B3", "UniRef:UniRef100_UPI0002F596B3", "UniRef:UniRef50_UPI0002D36BE5", "UniRef:UniRef90_UPI0002D36BE5" ], "nt": "ATGCAAAAGTTTAAACAACTGGTTGGTGCAATGCACAGATGGGTCAAACTGGCACTATTAGTAATCATTGTGTTATTAGGGATTATCTTTTGTCTGTTTGCCATCTATGGCATTGCGCAAGTGATCATTACCATTAATGAAGGGGCACTTTTATAA", "aa": "MQKFKQLVGAMHRWVKLALLVIIVLLGIIFCLFAIYGIAQVIITINEGALL", "aa_hexdigest": "abfd7d7135376c1e123c969cd89ed0d9", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002F596B3", "ncbi_nrp_id": "WP_158309334.1", "uniref100_id": "UniRef100_UPI0002F596B3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002F596B3", "RefSeq:WP_158309334.1", "UniRef:UniRef100_UPI0002F596B3" ] }, "ips": { "uniref100_id": "UniRef100_UPI0002F596B3", "uniref90_id": "UniRef90_UPI0002D36BE5" }, "psc": { "uniref90_id": "UniRef90_UPI0002D36BE5", "uniref50_id": "UniRef50_UPI0002D36BE5" }, "pscc": { "uniref50_id": "UniRef50_UPI0002D36BE5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D36BE5" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5697.091199999998, "isoelectric_point": 9.626564216613769 }, "id": "JPCNHPFPBM_433", "locus": "GCA000292505_00397" }, { "type": "cds", "sequence": "contig_1", "start": 220402, "stop": 221169, "strand": "+", "frame": 1, "gene": "nrnA", "product": "Bifunctional oligoribonuclease and PAP phosphatase NrnA", "db_xrefs": [ "COG:COG0618", "COG:FT", "EC:3.1.-.-", "GO:0003676", "GO:0004527", "GO:0008441", "KEGG:K06881", "SO:0001217", "UniRef:UniRef50_P75144", "UniRef:UniRef90_P22746" ], "nt": "ATGAAAAAAGGATCAATAACTGAAGCAATTAATGCCATTAAACAATTTGATAAGATCGTTATCTTTCACCATGTGCGCCCTGACGGGGATTGTTTAGGAGCACAACAAGGTTTGTTTCACCTCATTAAAGCTAACTTTAAAGATAAGGAGGTGAAGTGTGTTGGTAATAACAACAACCTGTTTAGCTTTATCAACATGACATTTACCAACCAAATTGATGAGAGCTTTTTAAAAGAAGCACTTGCGATTGTGGTCGATGCTAATTACAAAAACAGGATTGAATTGAGAGAACTGTTAGATAAAAACCTGTTTAAAGCAGTGTTAAGGATTGATCACCATCCCAATGAAGATGATCTAAACACTAGCTTTAACTTTGTTGAAGAAAGCTATGTAGCTTGTTGTGAGCAGATAGTGGAGATGGCCACAGTGGCGAAGTGGACCATACCACCAGTGGCTGCTACTTTACTATATATAGGTATCTATACGGATAGTAATAGATTTCTATATAGTAATACATCATATAGAACACTATACTTAGCAGCAATACTATATAAAGCTAAAGCTGATATAAGGATAGTACATGATCATTTAAACCATACTAGTTTAGCAGATCTTAAGTTTAAAAAGTATGTTTATAACCACTTTAAAACCCAAGGACAAGTGATCTATTTTATCTGTACTAAAAAGATCCAAAAGAGACTAAGAATGACTGCAGATCAATGTGCTAGAGTTAACTTGTTAAGTAACATAGCAGATTACAAGATCTGA", "aa": "MKKGSITEAINAIKQFDKIVIFHHVRPDGDCLGAQQGLFHLIKANFKDKEVKCVGNNNNLFSFINMTFTNQIDESFLKEALAIVVDANYKNRIELRELLDKNLFKAVLRIDHHPNEDDLNTSFNFVEESYVACCEQIVEMATVAKWTIPPVAATLLYIGIYTDSNRFLYSNTSYRTLYLAAILYKAKADIRIVHDHLNHTSLADLKFKKYVYNHFKTQGQVIYFICTKKIQKRLRMTADQCARVNLLSNIADYKI", "aa_hexdigest": "88b531f7a26b98e01ab4777f5ecb5743", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P22746", "query_cov": 1.0, "subject_cov": 0.8018867924528302, "identity": 0.996, "score": 510.0, "evalue": 6e-182, "valid": true, "gene": "nrnA", "product": "Bifunctional oligoribonuclease and PAP phosphatase NrnA", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75144", "cog_id": "COG0618", "cog_category": "FT", "kegg_orthology_id": "K06881", "go_ids": [ "GO:0008441", "GO:0004527", "GO:0003676" ] }, "pscc": { "uniref50_id": "UniRef50_P75144", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75144" ], "product": "Bifunctional oligoribonuclease and PAP phosphatase NrnA" }, "genes": [ "nrnA" ], "id": "JPCNHPFPBM_434", "locus": "GCA000292505_00398" }, { "type": "cds", "sequence": "contig_1", "start": 221806, "stop": 222333, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "ATGGAAAACCCCTCAACGGTGCAAAGGGGTTTAAATGGCGAGCCTATCTTTGATCCTTTTAAAGGCTTTGGTCTAACTGGTAATGCCCCTACTGATTGGAATGAGATCAAAGGTAAAGTTCCAGTAGAAGTAGTTCAATCCCCCCATTCCCCCAACCTCTATTTTGTGTTACTAGTGCCTAAGGTGGTAGTGGAGTACCACAAGCTCAGTAAGAAGGTTGTCAAAGAGAGTTTGGAAGTGGAAGCAACTGATTCCTTTGATCCCACCCAAAGGTTGAAAAAAGATAGTCCAGTGAAGGATTCAACAAAAACAGGGGAGAAACTCGAAACAACTGCTTCATCGATGAGTAGTGGTATGGCTACATCCACTCGCAAGGCCCTCAAAGTGGAGGTGGAAAGGGGGAGTAAAGTCAATCAAGGCGAACTACAATCCAACGACTTTGCTAAAAAGCCACTAAAGCATAAGAACAGTAGTGGGACAGATGTGAAGTTAGATTCACAGAAGGATTTTACTGAAGGAAAGGTTTGA", "aa": "MENPSTVQRGLNGEPIFDPFKGFGLTGNAPTDWNEIKGKVPVEVVQSPHSPNLYFVLLVPKVVVEYHKLSKKVVKESLEVEATDSFDPTQRLKKDSPVKDSTKTGEKLETTASSMSSGMATSTRKALKVEVERGSKVNQGELQSNDFAKKPLKHKNSSGTDVKLDSQKDFTEGKV", "aa_hexdigest": "0e07c82453c67888981c36a2a555c808", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 80, "aa_cov": 0.45714285714285713, "hmm_cov": 0.775, "evalue": 1.0113430831666994e-18, "score": 62.8036994934082, "start": 9, "stop": 88 } ], "seq_stats": { "molecular_weight": 19220.511099999992, "isoelectric_point": 8.942038917541506 }, "id": "JPCNHPFPBM_435", "locus": "GCA000292505_00399" }, { "type": "cds", "sequence": "contig_1", "start": 222340, "stop": 222492, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "GTGTTGACTACGGATGAGATAGAAAAAAATAGGGGGATGGGGGCGACGGTGGTTAGTTTCTATGATGCACCCTACAGTGAAAACCATACTGCCTTTGGACTTGTTGATCACATCGATCCTAAAAAGATGGTTGAAAACTACCCACCAAGTTGA", "aa": "MLTTDEIEKNRGMGATVVSFYDAPYSENHTAFGLVDHIDPKKMVENYPPS", "aa_hexdigest": "0f3e5e640f77182481be5a8ae4a1620a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 50, "aa_cov": 0.96, "hmm_cov": 1.0, "evalue": 9.975279252146887e-12, "score": 39.85225296020508, "start": 3, "stop": 50 } ], "seq_stats": { "molecular_weight": 5617.215400000001, "isoelectric_point": 4.734824562072753 }, "id": "JPCNHPFPBM_436", "locus": "GCA000292505_00400" }, { "type": "cds", "sequence": "contig_1", "start": 222547, "stop": 222888, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "TTGTTACAAACAACAGGGTTCTTTAACCCAAGAAGACACCCAGAGTGGTTTGATGAAGGACAAGCTAAGGCAGATAACACTAGCCCTGGCTTTAAGGTAGGGGAAAGTGATCACAAAAAAGACGGCTTTAAAGACTCTTCTTCTTCTTCTTCTTCTCCAATAGCTTTACCATTTGAAGCATACTTTGCTAATATTGGTAACATGGTTGCTATTGGCAACTCGGTATTTATCTTTGGTGGTAATGGTCATGCTACTAAGATGTTTACCACCAATCCCTTAAGTATTGGGGTATTTAGGATTAAATACACTGATAACTTTAGTAAGTCATCAGTAACTGGTTGA", "aa": "MLQTTGFFNPRRHPEWFDEGQAKADNTSPGFKVGESDHKKDGFKDSSSSSSSPIALPFEAYFANIGNMVAIGNSVFIFGGNGHATKMFTTNPLSIGVFRIKYTDNFSKSSVTG", "aa_hexdigest": "6483db84a7afcb3ad871d6fbce848b7c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 110, "aa_cov": 0.9734513274336283, "hmm_cov": 0.7727272727272727, "evalue": 3.7469319730850154e-34, "score": 113.44246673583984, "start": 4, "stop": 113 } ], "seq_stats": { "molecular_weight": 12278.497099999986, "isoelectric_point": 8.04483242034912 }, "id": "JPCNHPFPBM_437", "locus": "GCA000292505_00401" }, { "type": "cds", "sequence": "contig_1", "start": 222898, "stop": 223170, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12378.14" ], "nt": "GTGTTATTTGGAGGATTAATTAATCCCCAAACCAATGGCTTGAAGGATCTTCCCCTTGGTACCAACAGGTGGTTTGAATATGTACCAAGAATGGCAGTTAGTGGGGTAAAATGGGTTGGTAATCAACTAGTGTTAGCAGGAACACTAACAATGGGTGATACAGCTACTGTACCTAGGTTAAAGTATGATCAACTAGAAAAACACTTAAACCTAGTTGCTCAAGGCCAGGGACTATTGAGAGAAGACTTGCAGATCTTCACTCCCTATGGGTGA", "aa": "MLFGGLINPQTNGLKDLPLGTNRWFEYVPRMAVSGVKWVGNQLVLAGTLTMGDTATVPRLKYDQLEKHLNLVAQGQGLLREDLQIFTPYG", "aa_hexdigest": "906032a5b7f3406e7d53e54cdf52c5f9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12378.14", "name": "P1_N", "length": 36, "aa_cov": 0.3888888888888889, "hmm_cov": 1.0, "evalue": 2.790245888923363e-10, "score": 35.104736328125, "start": 1, "stop": 35 } ], "seq_stats": { "molecular_weight": 9986.4679, "isoelectric_point": 8.085705375671388 }, "id": "JPCNHPFPBM_438", "locus": "GCA000292505_00402" }, { "type": "cds", "sequence": "contig_1", "start": 223216, "stop": 223371, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCAGTAAATTTGGTCCCCATTACTTTTTGAATAACCCTGATATCCAGGACAATGTTAATAATGATACGGTTGAAGCATTAATCAATACCTATACAAACACTGATAAGTTAAAACACGTTTATCCTTATAGATACAGTGGTTTGTATGCTTGA", "aa": "MGSKFGPHYFLNNPDIQDNVNNDTVEALINTYTNTDKLKHVYPYRYSGLYA", "aa_hexdigest": "d1ecebc692221f298043085ffa877dc7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5913.454400000001, "isoelectric_point": 5.973914527893067 }, "id": "JPCNHPFPBM_439", "locus": "GCA000292505_00403" }, { "type": "cds", "sequence": "contig_1", "start": 223456, "stop": 223599, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGCTGCTATCTTAAATGAAGATCTGTTAACAGGGCTAAGTGATAAGATTGGCTATGGTAAGGAGAATGAGTTTGCTGATAATGAAGCAGATAGGTTTAACCAACTTTTAAGTTTAAATCCTAGTGATAACACCAACTGA", "aa": "MFAAILNEDLLTGLSDKIGYGKENEFADNEADRFNQLLSLNPSDNTN", "aa_hexdigest": "e5c2d66d4ea713b430bd0db955407810", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5219.615400000001, "isoelectric_point": 4.0500284194946286 }, "id": "JPCNHPFPBM_440", "locus": "GCA000292505_00404" }, { "type": "cds", "sequence": "contig_1", "start": 223665, "stop": 223799, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCATTGATTTAACCCCGACATTGATGTTAGAAAAAGTGGGGAGGGGGGAAGAAGAAGAAGAAAGGGTAGCAGTGTGGGAGTTGGAGAAGGGTTTGTTGTTGCCGATTCAGGGGAGAAAGTCTAAGAACTGA", "aa": "MIIDLTPTLMLEKVGRGEEEEERVAVWELEKGLLLPIQGRKSKN", "aa_hexdigest": "8545206389dae848e4543d2bfe8be439", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5049.8623, "isoelectric_point": 4.9672960281372065 }, "id": "JPCNHPFPBM_441", "locus": "GCA000292505_00405" }, { "type": "cds", "sequence": "contig_1", "start": 223800, "stop": 223907, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCGCTGGTGTTTGATGCATCCTTGCGATACCCAGCCCCAGTTCAAATGTCTGGTGAAGAGTTAGGGGTAAACACCCACCGGGTGTTTTCTTTGTTTAAGTGTTGA", "aa": "MPLVFDASLRYPAPVQMSGEELGVNTHRVFSLFKC", "aa_hexdigest": "98fbedcfc841e8bc3ff6fca4f53328d4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3940.5692, "isoelectric_point": 6.511497688293456 }, "id": "JPCNHPFPBM_442", "locus": "GCA000292505_00406" }, { "type": "cds", "sequence": "contig_1", "start": 223921, "stop": 224412, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGCCTAGTAGTAGTAGTAGTAGTAGTAGTCCTTCGTTTAATCCCTCCTCTGCTGAAAATCAAGTCACCCCAGCTGGTGGCTCCTCCAAAAAACCAACCACCTACACCCATTTACCCAACAGTATCAGTCCCACCAGTGACTGGATCAACGCATTAACCTTCACCAATAAGAATAATCCGCAGCGCAATCAGTTGTTGTTAAGAGCACTGTTAGGAACGATCCCGGTCTTGATCAATAAGAGTGGAGGAAGTGGGAATGAGTTTAACCATACGAATGATCAGAAGTGGGATAAAACTGAGACAAATGAGGGCAACCTCCCGGGGTTTGGGGAGGTGAATGGGTTGTATAATGCCGCATTACTTTATACCTATGGTTTTTTTGGCACCAATACCAATAACTCAGATCCCAAAATAGGCTTTAAAGCTGATAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTACACTAGTAGGTAGTGGGTTAAACTGA", "aa": "MLPSSSSSSSSPSFNPSSAENQVTPAGGSSKKPTTYTHLPNSISPTSDWINALTFTNKNNPQRNQLLLRALLGTIPVLINKSGGSGNEFNHTNDQKWDKTETNEGNLPGFGEVNGLYNAALLYTYGFFGTNTNNSDPKIGFKADSSSSSSSSSSSTLVGSGLN", "aa_hexdigest": "8aea01cc11724e3b9e76c9ce9f6e256a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17129.357499999987, "isoelectric_point": 6.7162885665893555 }, "id": "JPCNHPFPBM_443", "locus": "GCA000292505_00407" }, { "type": "cds", "sequence": "contig_1", "start": 224824, "stop": 225744, "strand": "+", "frame": 1, "gene": "mgpA", "product": "adhesin P1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002244F655", "UniRef:UniRef90_UPI002244F655" ], "nt": "ATGAATAACTTGTTAAAACTACTGACAACTAAAAATGTGAAAGCAAAGTTGGGGAAGGGAGCAGCTTCTTCGCAGGGAAGTGGGGGGGGTGTTAGTCAAACGATTAATACCATCGCTACTACGGGGAATATTAGTGAAGGTCTAAAAGAAGAAACTAGTATTCAAGCAGAAACACTTAAAAAGTTCTTTGATAGTAAACAAAACAATAAGAGTGAAATAGGGATAGGTGATAGTACATTTACCAAGATGGATGGTAAACTAACTGGCGTAGTATCTACTCCTCTTGTTAACCTTATCAATGGTCAGGGAGCAACTAGTGATAGTGATACTGAAAAAATTAGCTTTAAACCTGGTAACCAGATTGACTTTAATAGGTTATTTACCTTACCAGTAACTGAACTATTTGATCCTAACACGATGCTTGTCCATGACCAGTATGTACCACTATTGGTTAACTTACCTAGTGGCTTTGATCAAGCTTCAATCCGCTTAAAGGTAATTAGTTACTCAGTAGAAAACCAAACCTTAGGAGTAAGATTAGAGTTCAAAGATCCTGATACTAACCAGTTTATTCCGGTACTAAATGCATCAAGTACTGGTCCCCAAACTGTCTTTCAACCCTTTAATCAGTGGGCAGACTATGTCTTACCTTTGATTGTAACTGTTCCTATAGTAGTGATTATCCTTAGTGTTACTTTGGGATTAACGATTGGAATTCCAATGCACAGAAACAAAAAGGCATTACAAGCAGGGTTTGATCTTTCTAACAAAAAGGTTGATGTTTTGACCAAAGCAGTTGGTAGTGTCTTTAAAGAGATCATTAACAGAACAGGGATCTCTAACGCTCCTAAGAAGTTAAAACAAGCTACACCAACCAAACCAACTCCTAAAACCCCACCAAAACCTCCAGTAAAACAATAA", "aa": "MNNLLKLLTTKNVKAKLGKGAASSQGSGGGVSQTINTIATTGNISEGLKEETSIQAETLKKFFDSKQNNKSEIGIGDSTFTKMDGKLTGVVSTPLVNLINGQGATSDSDTEKISFKPGNQIDFNRLFTLPVTELFDPNTMLVHDQYVPLLVNLPSGFDQASIRLKVISYSVENQTLGVRLEFKDPDTNQFIPVLNASSTGPQTVFQPFNQWADYVLPLIVTVPIVVIILSVTLGLTIGIPMHRNKKALQAGFDLSNKKVDVLTKAVGSVFKEIINRTGISNAPKKLKQATPTKPTPKTPPKPPVKQ", "aa_hexdigest": "6f4c849f8c2f0c7fedf33c937b174156", "start_type": "ATG", "rbs_motif": null, "genes": [ "mgpA" ], "truncated": "5-prime", "pseudogene": { "start": 224524, "stop": 225744, "observations": { "mutation": [ 224566, 224581, 224635, 224797 ], "stop": [ 224566, 224581, 224635, 224797 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002244F655", "query_cov": 1.0032679738562091, "subject_cov": 0.5099667774086378, "identity": 0.941, "score": 530.0, "evalue": 1.17e-184, "gene_start": 1, "gene_end": 306, "reference_start": 296, "reference_end": 602, "reference_sequence": "MASLLKSVKPSNGSTSFNPTSMENQVTPSGGSSKKTTYTHLPNSISPTSDWINALTFTNKNNPQRNQLLLRALLGTIPVLINKSGDSNDQFNKDSEQQWNETEKPNGNLPGFGEVNGLYNAALLHTYGFFGTNTNSTDPKIGFKADSSSSSSTLVGSGLNWTSQDVGNLVVINDTSFGFQLGGWFITFTDFIRPRTGYLGITLSSLQDQTIIWADQPWTSFKGSYLDSDGTPKSLWDPTALKSLPNSSTTYDTNPTLSPSFLYFQPNKVKSGQYQQNNTYNKLIEPDNATSAATNMTNLLKLLSSKNIKQKLGKDTQSMGNNNGGGVSQTINTITTTGNISEGLKEETSIQAETLKKFFDSKQNNKSEIGIGDSTFTKMDGKLTGVVSTPLVNLINGQGATSDSDTEKISFKPGNQIDFNRLFTLPVTELFDPNTMFVYDQYVPLLVNLPSGFDQASIRLKVISYSVENQTLGVRLEFKDPQTQQFIPVLNASSTGPQTVFQPFNQWADYVLPLIVTVPIVVIILSVTLGLTIGIPMHRNKKALQAGFDLSNKKVDVLTKAVGSVFKEIINRTGISNAPKKLKQATPTKPTPKTPPKPPVKQ" }, "paralog": false, "identity": 0.9017199017199017, "score": 685.0, "evalue": 4.84e-248, "description": "Internal stop codon at 224566, 224581, 224635, 224797. Nonsense mutation around 224566, 224581, 224635, 224797.", "psc": { "uniref90_id": "UniRef90_UPI002244F655", "gene": "mgpA", "product": "adhesin P1", "uniref50_id": "UniRef50_UPI002244F655", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002244F655", "UniRef:UniRef50_UPI002244F655" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002244F655", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002244F655" ], "product": "adhesin P1" } }, "id": "JPCNHPFPBM_444", "locus": "GCA000292505_00408" }, { "type": "cds", "sequence": "contig_1", "start": 225746, "stop": 226114, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACAATAAGAAAACAGATTTATAAAAAAGCATACTGGTTACTATTACCCTTTCTACCATTAGCACTAGCCAATACCTTCCTTGTCAAAGAGGATAGTAAGAATGTTACTGCTTACACTCCCTTCGCCACCCCCATCACCGATTCTAAGGCTGATCTGGTTAGTTTGGCACAACTTGATTCTTCCTATCAAATCGCTGACCAAACCATCCATAACACCAACTTGTTTGTGTTGTTCAAGTCTAGGGATGTGAAGCTTACATACAGTTCAAGTGGCTCAAATAACCAGATTAGTTTTGATTCAACTAACAACAAACCATCCTACATCGTCGAGTTTACTAACTCCACTACGGTAGGCATCAAGTGA", "aa": "MKTIRKQIYKKAYWLLLPFLPLALANTFLVKEDSKNVTAYTPFATPITDSKADLVSLAQLDSSYQIADQTIHNTNLFVLFKSRDVKLTYSSSGSNNQISFDSTNNKPSYIVEFTNSTTVGIK", "aa_hexdigest": "98ae47fca1550afcb488727f82df971a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13707.453499999983, "isoelectric_point": 9.331170082092285 }, "id": "JPCNHPFPBM_445", "locus": "GCA000292505_00409" }, { "type": "cds", "sequence": "contig_1", "start": 226121, "stop": 226294, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13", "UniRef:UniRef90_UPI0030D43D23" ], "nt": "GTGAAAAAGTATCAGTTAGATGTGCCAAGTGTTTCAACAACCATGAACCAAGTTTTGCAAGAATTGATCCTAGAACAACCTTTGACTAAGTATACGCTTAATAGTAGTTTGGCCAAACAAAAGGGCAAAACGCAAAGGGAGGTGCATCTTGGTTCAAATATGGCAAATCAGTGA", "aa": "MKKYQLDVPSVSTTMNQVLQELILEQPLTKYTLNSSLAKQKGKTQREVHLGSNMANQ", "aa_hexdigest": "c01d0aeeb65f905264a6e77fa916ae1d", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 225998, "stop": 226359, "observations": { "deletion": [ 226316 ], "mutation": [ 226112, 226292 ], "stop": [ 226112, 226292 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0030D43D23", "query_cov": 1.0, "subject_cov": 0.47107438016528924, "identity": 0.825, "score": 89.0, "evalue": 1.34e-21, "gene_start": 1, "gene_end": 57, "reference_start": 43, "reference_end": 99, "reference_sequence": "MKLTYSSSGSNTNTISFENNANKPSYVVEFTNSTNVGIKWRVVKKYQLDVPNVSSDMNQVLQELILEQPLTKYTLNSSLAKQKGKSKIEVHLGSGRATQWTSRTQLHWPKQQSQPKCINRI" }, "paralog": false, "identity": 0.7272727272727273, "score": 156.0, "evalue": 6.4e-51, "description": "Internal stop codon at 226112, 226292. Frameshift due to deletion around 226316. Nonsense mutation around 226112, 226292.", "psc": { "uniref90_id": "UniRef90_UPI0030D43D23", "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0030D43D23", "UniRef:UniRef50_UPI00027B3D13" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6462.389900000002, "isoelectric_point": 9.52289905548096 }, "id": "JPCNHPFPBM_446", "locus": "GCA000292505_00410" }, { "type": "cds", "sequence": "contig_1", "start": 226301, "stop": 226402, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTAATCAACACAACTTAGACAACAATCCCTCTCCCAATGCATCAACTGGATTTAAACTCACTACCGGCAACGCATATAGAAAATTGGATCAATCCTGA", "aa": "MRNQHNLDNNPSPNASTGFKLTTGNAYRKLDQS", "aa_hexdigest": "9f7100dd3d1242c098ec61834ea7e5b5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3690.967600000001, "isoelectric_point": 9.69399814605713 }, "id": "JPCNHPFPBM_447", "locus": "GCA000292505_00411" }, { "type": "cds", "sequence": "contig_1", "start": 226481, "stop": 226783, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGCAGGGGATGCGCCCAGTGTTACTGCAGGGGGAAGTGCTTCTGGATCATTTAATAAATACCTCAACACCAAACAAGCGTTAGAAAGGATCGGCATCTTGTTTGATGGGGATGGAATGAGGAATGTTATCACCCAGCTCTATTATGCTTCTACCAGTAAGCTAGCAGTCACTAACAACCACATTGTCGTGATGGGTAACAGCTTTCTACCCAGCTTGTGGTACTGGGTGGTGGATCGGGGTGCAACAACTGATTCATCATCAAAACCCACCTGGTTTGCTAACACCAATTTAGATTGA", "aa": "MAAGDAPSVTAGGSASGSFNKYLNTKQALERIGILFDGDGMRNVITQLYYASTSKLAVTNNHIVVMGNSFLPSLWYWVVDRGATTDSSSKPTWFANTNLD", "aa_hexdigest": "22a2f00ea51663f38740831e092ed841", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10753.904099999992, "isoelectric_point": 6.532698631286621 }, "id": "JPCNHPFPBM_448", "locus": "GCA000292505_00412" }, { "type": "cds", "sequence": "contig_1", "start": 226820, "stop": 227011, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05220.18" ], "nt": "TTGGGGTATAAGGAAACTACCAGTACCAATTCCCACAACTTCCATTCCAAATCTTTCACCCAACCTGCATATCTGATCAGTGGCATTGACAGTGTCAATGATCAGTTGATCTTCAGTGGTTTTAAAGCAGGGAGTGTGGGGTATGATAGTAGTAGTAGTAGTACTCAAACCAAAGACCAAGCACTTGCTTGA", "aa": "MGYKETTSTNSHNFHSKSFTQPAYLISGIDSVNDQLIFSGFKAGSVGYDSSSSSTQTKDQALA", "aa_hexdigest": "0d6d85112416cff956c1561f38cc4676", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05220.18", "name": "MgpC", "length": 66, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 8.336959789947662e-19, "score": 62.90186309814453, "start": 1, "stop": 63 } ], "seq_stats": { "molecular_weight": 6741.201300000003, "isoelectric_point": 5.974028205871583 }, "id": "JPCNHPFPBM_449", "locus": "GCA000292505_00413" }, { "type": "cds", "sequence": "contig_1", "start": 227057, "stop": 227449, "strand": "+", "frame": 2, "gene": "mgpC", "product": "MgpC protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8G0" ], "nt": "GTGACCAACGACACGGGTTTAAATGGTCCAATCAATGGGAGTTTTTCAATCCAAGACACCTTCAGCTTTGTTGTTCCTTATTCGGGGAATCATAACAATAATGGAAATATCAGTGGCAATGGAACCATTCAAACGGCTTATCCGGTGAAAAAAGATCAAAAATCAACAGTAATGATCAATTCTTTGATCAACGCTACGCCGTTGAATAGTTATGGGGATGAGGGGGTTGGGGTGTTTGATGCGTTAGGTTTAAACTATAACTTTAAATCTAACCAAGAACGTTTACCTTCCAGAACTGATCAGATCTTTGTTTATGGGATTGTCTCCCCTAATGAATTGCGAAGTGCTAAAAGTTCTGCTGATTCAACTGGTAGTGATACAAAGGTAAACTGA", "aa": "MTNDTGLNGPINGSFSIQDTFSFVVPYSGNHNNNGNISGNGTIQTAYPVKKDQKSTVMINSLINATPLNSYGDEGVGVFDALGLNYNFKSNQERLPSRTDQIFVYGIVSPNELRSAKSSADSTGSDTKVN", "aa_hexdigest": "0216a2e53ed38f275c86b8fd5bb26956", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F305329", "query_cov": 0.8846153846153846, "subject_cov": 1.0087719298245614, "identity": 0.6, "score": 138.0, "evalue": 4.88e-40, "valid": false, "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_A0AB38W8G0" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W8G0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W8G0" ], "product": "MgpC protein" }, "genes": [ "mgpC" ], "id": "JPCNHPFPBM_450", "locus": "GCA000292505_00414" }, { "type": "cds", "sequence": "contig_1", "start": 227654, "stop": 228892, "strand": "+", "frame": 2, "gene": null, "product": "adhesin P110", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0015882A22", "UniRef:UniRef90_UPI002FCE5F12" ], "nt": "ATGAGTGGTAAGAAAGCTAACTATGGAGCGGTTGTGTTAACACGGGGGGGTGTTGTTAGATTAAACTTTAACCCTGGTAATGATTCATTGCTTTCAACAACTGATAACAATATAGCACCTATCTCCTTCTCATTTACTCCGTTCACAGCTGCTGAGAGTGCAGTGGATCTCACTACCTTCAAAGAAGTTACCTATAACCAAGAATCAGGATTGTGGAGTTATGTCTTTGACAACTCCTTAAAACCAACCCATGATGGTAAACAAATTGCTGTCACTGATAACAAGGGCTTTAGTGTTATCACTGTCTCAAGAACTGGCATTGAACTAAACCAAGACCAAGCTACTACAACTCTTGATGTAGCACCTAGTGCACTAGCAGTGCAATCAGGGATCCAATCTACCACCCAAACCCTAACTGGAGTACTCCCACTTAGTGAGGAATTCAGTGCAGTTATTGCTAAAGATAGTGGTCAAAATAAGATTGATATCTATAAAAACAACAACGGGTTGTTTGAAATTGATACCCAACTAAGTAATAGTGTTGCCACCAACAACGGTGGGTTAGCACCTAGTTACACAGAAAACACGGTTGATGCATGGGGTAAAGTTGAGTTTGCTGATAACAGTGTATTGCAAGCAAGAAACCTAGTTGATAAAACTGTTGATGAGATCATCAATACCCCTGAAATCTTAAATTCCTTCTTTAGATTCACCCCTGCTTTTGAAGATCAAAAAGCTACCCTTGTTGCTACTAAGCAAAGTGATACGTCACTTAGTGTCTCACCAAGAATCCAGTTCTTAGATGGTAATTTCTATGATCTTAACTCCACCATCGCTGGGGTACCTTTAAACATTGGTTTCCCTTCAAGAGTGTTTGCTGGGTTTGCAGCACTCCCTGCATGGGTGATCCCTGTATCAGTAGGTTCTTCAGTTGGGATCTTGTTTATCTTGTTAGTCTTAGGACTTGGGATTGGGATCCCAATGTACAGGGTAAGAAAACTCCAAGATGCATCGTTTGTTAATGTCTTTAAAAAGGTTGATACACTCACAACTGCTGTCGGTAGTGTGTACAAAAAGATTATTACCCAAACTGGTGTGGTGAAAAAAGCACCTAGTGCATTGAAAGCTGCTAATCCTAGTGTTAAAAAACCAGCAGCCAAACCACCTGTTCAACCACCAAGTAAACCTGAAGGGGAACAAAAAGCTGTTGAAGTTAAGTCAGAGGAAACCAAAAGTTAG", "aa": "MSGKKANYGAVVLTRGGVVRLNFNPGNDSLLSTTDNNIAPISFSFTPFTAAESAVDLTTFKEVTYNQESGLWSYVFDNSLKPTHDGKQIAVTDNKGFSVITVSRTGIELNQDQATTTLDVAPSALAVQSGIQSTTQTLTGVLPLSEEFSAVIAKDSGQNKIDIYKNNNGLFEIDTQLSNSVATNNGGLAPSYTENTVDAWGKVEFADNSVLQARNLVDKTVDEIINTPEILNSFFRFTPAFEDQKATLVATKQSDTSLSVSPRIQFLDGNFYDLNSTIAGVPLNIGFPSRVFAGFAALPAWVIPVSVGSSVGILFILLVLGLGIGIPMYRVRKLQDASFVNVFKKVDTLTTAVGSVYKKIITQTGVVKKAPSALKAANPSVKKPAAKPPVQPPSKPEGEQKAVEVKSEETKS", "aa_hexdigest": "6316e5218a4af8419d42a56da383639c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 227354, "stop": 228892, "observations": { "mutation": [ 227447 ], "stop": [ 227447 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FCE5F12", "query_cov": 1.0, "subject_cov": 0.5778401122019635, "identity": 0.995, "score": 752.0, "evalue": 1.81e-268, "gene_start": 1, "gene_end": 412, "reference_start": 302, "reference_end": 713, "reference_sequence": "NNKTNWFDNNNVDWGENKKKQCDENQLGYKETTSTNSHNFHSKSFTQPAYFISGIDSVNDQLIFSGFKAGSVGYDSSSSSSSTKDQALAWSTTTSLDSKTGYRDLVTNDTGLNGPINGSFSIQDTFSFVVPYSMNHTNTGTSGPIKTAYPVKKSEASTVMINSLINATPLNSYGDEGVGVFDALGLNYNFKSNQERLPSRTDQIFVYGIVSPNELRSAKSSADSTGSDTKVNWSNTQSRYLPVPYNYSEGIIDADGFKRPENRGASVTTFSGLKSIAPDGFANSIANFSVGLKAGIDPNPVMSGKKANYGAVVLTRGGVVRLNFNPGNDSLLSTTDNDVAPISFSFTPFTAAESAVDLTTFKEVTYNQESGLWSYVFDNSLKPTHDGKQIAVTDNKGFSVITVSRTGIELNQDQATTTLDVAPSALAVQSGIQSTTQTLTGVLPLSEEFSAVIAKDSGQNKIDIYKNNNGLFEIDTQLSNSVATNNGGLAPSYTENTVDAWGKVEFADNSVLQARNLVDKTVDEIINTPEILNSFFRFTPAFEDQKATLVATKQSDTSLSVSPRIQFLDGNFYDLNSTIAGVPLNIGFPSRVFAGFAALPAWVIPVSVGSSVGILFILLVLGLGIGIPMYRVRKLQDASFVNVFKKVDTLTTAVGSVYKKIITQTGVVKKAPSALKAANPSVKKPAAKPPVQPPSKPEGEQKAVEVKSEETKS" }, "paralog": false, "identity": 0.9921875, "score": 969.0, "evalue": 0.0, "description": "Internal stop codon at 227447. Nonsense mutation around 227447.", "psc": { "uniref90_id": "UniRef90_UPI002FCE5F12", "product": "adhesin P110", "uniref50_id": "UniRef50_UPI0015882A22", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FCE5F12", "UniRef:UniRef50_UPI0015882A22" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0015882A22", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0015882A22" ], "product": "Mgp-operon protein 3" } }, "id": "JPCNHPFPBM_451", "locus": "GCA000292505_00415" }, { "type": "cds", "sequence": "contig_1", "start": 229653, "stop": 229823, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCATTGATTTAACCCCAACATTGATGTTAGAAAAAGTGGGGAGTGGGGTATTTGAACTAACAGAAAGGGTAGCAGTGTGGGAGTTGGAGAAGGGTTTGTTGTTGCCGATTCAGGGGAGAAAGTCTAAGTCGCCCCCATCCCCCTATTTTTTGAAAGTTGGTCCGTAG", "aa": "MIIDLTPTLMLEKVGSGVFELTERVAVWELEKGLLLPIQGRKSKSPPSPYFLKVGP", "aa_hexdigest": "001dfaf755ca09397193ccab00553420", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6210.350200000001, "isoelectric_point": 8.099952888488769 }, "id": "JPCNHPFPBM_452", "locus": "GCA000292505_00416" }, { "type": "cds", "sequence": "contig_1", "start": 230082, "stop": 230315, "strand": "+", "frame": 3, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49206", "UniRef:UniRef90_UPI00027B3795" ], "nt": "TTGACTTTCACTAATAAGAACAACCCGCAGAGAAATCAACTGTTGTTAAGAGCGTTATTAGGAACTATCCCGGTCTTGATCAATAAGAGTGGGGAGGGTGGGGAAGAGTTTACCAAGGATAGTGAGCAGAAGTGGGATAAGACAGAAACCAAAGATGGCAACCTCCCGGGGTTTGGGGAGGTGAATGGTGGTTTTTATCAACTAAATAAAAACTTATTAGCTTATTTTTATTAG", "aa": "MTFTNKNNPQRNQLLLRALLGTIPVLINKSGEGGEEFTKDSEQKWDKTETKDGNLPGFGEVNGGFYQLNKNLLAYFY", "aa_hexdigest": "aea75a2fb249400cf33b202807054a76", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 229944, "stop": 230315, "observations": { "mutation": [ 230070 ], "stop": [ 230070 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00027B3795", "query_cov": 1.0, "subject_cov": 0.6260162601626016, "identity": 0.987, "score": 160.0, "evalue": 1.59e-49, "gene_start": 1, "gene_end": 77, "reference_start": 47, "reference_end": 123, "reference_sequence": "MKPSSSSTLFDPSSMENKVTSGGSSKPTTYTHLPNSISPTSDWSNALTFTNKNNPQRNQLLLRALLGTIPVLINKSGEGGEEFTKDSEQKWDKTETKDGNLPGFGEVNGGFYQLNKNLLAYFY" }, "paralog": false, "identity": 0.983739837398374, "score": 248.0, "evalue": 1.67e-86, "description": "Internal stop codon at 230070. Nonsense mutation around 230070.", "psc": { "uniref90_id": "UniRef90_UPI00027B3795", "product": "adhesin", "uniref50_id": "UniRef50_Q49206", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00027B3795", "UniRef:UniRef50_Q49206" ] }, "pscc": { "uniref50_id": "UniRef50_Q49206", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49206" ], "product": "Random genomic clone esh10, partial cds" } }, "id": "JPCNHPFPBM_453", "locus": "GCA000292505_00417" }, { "type": "cds", "sequence": "contig_1", "start": 230604, "stop": 230780, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13", "UniRef:UniRef90_UPI0030D43D23" ], "nt": "GTGGTGAAAAAGTATCAGTTAGATGTGCCAAGTGTTTCTAGCAACATGAACCAAGTTTTGCAAGAATTGATCCTTGAACAACCTTTGACTAAATATACCTTAAACAGTAGTTTGGCCAAACAAAAGGGCAAAACGCAAAGGGAGGTGCATCTTGGTTCAAATATGGCAAATCAGTGA", "aa": "MVKKYQLDVPSVSSNMNQVLQELILEQPLTKYTLNSSLAKQKGKTQREVHLGSNMANQ", "aa_hexdigest": "4926e316c4882225bfad7e409114a334", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 230484, "stop": 230845, "observations": { "deletion": [ 230802 ], "mutation": [ 230598, 230778 ], "stop": [ 230598, 230778 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0030D43D23", "query_cov": 0.9827586206896551, "subject_cov": 0.47107438016528924, "identity": 0.86, "score": 91.7, "evalue": 1.21e-22, "gene_start": 2, "gene_end": 58, "reference_start": 43, "reference_end": 99, "reference_sequence": "MKLTYSSSGSNTNTISFENNANKPSYVVEFTNSTNVGIKWRVVKKYQLDVPNVSSDMNQVLQELILEQPLTKYTLNSSLAKQKGKSKIEVHLGSGRATQWTSRTQLHWPKQQSQPKCINRI" }, "paralog": false, "identity": 0.7520661157024794, "score": 164.0, "evalue": 6.32e-54, "description": "Internal stop codon at 230598, 230778. Frameshift due to deletion around 230802. Nonsense mutation around 230598, 230778.", "psc": { "uniref90_id": "UniRef90_UPI0030D43D23", "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0030D43D23", "UniRef:UniRef50_UPI00027B3D13" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6560.493000000002, "isoelectric_point": 9.52289905548096 }, "id": "JPCNHPFPBM_454", "locus": "GCA000292505_00418" }, { "type": "cds", "sequence": "contig_1", "start": 230787, "stop": 230888, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTAATCAACACAACTTAGACAACAATCCCAGTCCCAATGCATCAACCGGATTTAAACTCGACAAAGGCAATGCATATAGAAAACTAAGTGAGTCCTGA", "aa": "MRNQHNLDNNPSPNASTGFKLDKGNAYRKLSES", "aa_hexdigest": "cb3032c4f22c019fe28c6017f37615c2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3704.994200000001, "isoelectric_point": 9.520965003967284 }, "id": "JPCNHPFPBM_455", "locus": "GCA000292505_00419" }, { "type": "cds", "sequence": "contig_1", "start": 231078, "stop": 231383, "strand": "+", "frame": 3, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FCAC30C", "UniRef:UniRef90_UPI00027B3797" ], "nt": "ATGGGGATGGAACGAGGAATGTTGTTAGCCTCCTTCCTCTACTTTCAACCCAACAAGGTGAAAAGTGGTCAATATCAACAAAATAACACCTACAACAGGTTAATTGAACCGGAAAGTGCAACAAGTGCAGCAACTAACATGACCAACTTGTTAAAACTACTGACAACTAAAAACATCAAAGAAAAGTTGGGGAAGGGAGCAGCTTCTTCGCAGGGAAGCAAGACACCTTTTCCTTCGTTGTTCCTTATTCAGATAGTCATAGTAATCAAACTTCATCAGGAACCATTAAAACGGCTTATCCTGTGA", "aa": "MGMERGMLLASFLYFQPNKVKSGQYQQNNTYNRLIEPESATSAATNMTNLLKLLTTKNIKEKLGKGAASSQGSKTPFPSLFLIQIVIVIKLHQEPLKRLIL", "aa_hexdigest": "3e5447856c163e1bb5a788d9232c3fdb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00027B3797", "query_cov": 1.0, "subject_cov": 0.926605504587156, "identity": 1.0, "score": 190.0, "evalue": 3.53e-61, "valid": true, "uniref50_id": "UniRef50_UPI001FCAC30C" }, "pscc": { "uniref50_id": "UniRef50_UPI001FCAC30C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FCAC30C" ], "product": "adhesin" }, "genes": [], "id": "JPCNHPFPBM_456", "locus": "GCA000292505_00420" }, { "type": "cds", "sequence": "contig_1", "start": 232118, "stop": 232678, "strand": "+", "frame": 2, "gene": "pheS", "product": "phenylalanine--tRNA ligase subunit alpha", "db_xrefs": [ "COG:COG0016", "COG:J", "EC:6.1.1.20", "GO:0000049", "GO:0000166", "GO:0000287", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432", "KEGG:K01889", "SO:0001217", "UniRef:UniRef50_P75564", "UniRef:UniRef90_P47436" ], "nt": "ATGCTACCAGCAACAGAGTTTACCAATGGTTCTAATAACGCACTATATCAGGTTATTGATAATTTAGTTGAATACTTTAAAAGCTTTTTATTCACAATTAATTTTGATAGTGAACTGACCAGTATTAGTGACTGTTTTGATCTTTTAAATATCCCTAAAGATCATTCCAGTAGGAATGAATCTGATTCTTTTTATATCGATAAAACCAGTTTATTGAGAACCCATTGTACTGCTACCACGCTAAAAGCAGTCAGAACTTCTAAAAAAACTAATAATCCTGATATCAGGGTTGTCTCTTTAGGAGCGGTTTTTCGTAATGATAGTGATGATGCCACCCACTCCCATCAGTTTACCCAACTTGATTTTATGTGGATTAAAAAAGGGCTTTCATTAGCTAATTTAAAGTGGTTTATTAACAATATGATCACCCATTTCTTTGGGGAAAATACTTTTACTAGGTTTAGACTATCCCACTTCCCATTCACTGAACCCTCGTTTGAAATTGACATTAGGTGTTGGTTATGTCAAAATGGTTGTTCTATTTGTAAGCAAACCAAGTGA", "aa": "MLPATEFTNGSNNALYQVIDNLVEYFKSFLFTINFDSELTSISDCFDLLNIPKDHSSRNESDSFYIDKTSLLRTHCTATTLKAVRTSKKTNNPDIRVVSLGAVFRNDSDDATHSHQFTQLDFMWIKKGLSLANLKWFINNMITHFFGENTFTRFRLSHFPFTEPSFEIDIRCWLCQNGCSICKQTK", "aa_hexdigest": "bf61341790ccddf0dd3cf8ca71deaf9c", "start_type": "ATG", "rbs_motif": null, "genes": [ "pheS" ], "truncated": "both", "pseudogene": { "start": 231845, "stop": 232870, "observations": { "mutation": [ 231875, 232676 ], "stop": [ 231875, 232676 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47436", "query_cov": 1.0, "subject_cov": 0.5454545454545454, "identity": 1.0, "score": 377.0, "evalue": 2.77e-130, "gene_start": 1, "gene_end": 186, "reference_start": 92, "reference_end": 277, "reference_sequence": "MIDQNKLITKWKKAFAKAKNLTTLVNLKNTLHNSDLKPLLQKIKTATKLSEKSSLGKLYQSLDIQLTDLLTSYKKTFEINNQVSQKPSLDVMLPATEFTNGSNNALYQVIDNLVEYFKSFLFTINFDSELTSISDCFDLLNIPKDHSSRNESDSFYIDKTSLLRTHCTATTLKAVRTSKKTNNPDIRVVSLGAVFRNDSDDATHSHQFTQLDFMWIKKGLSLANLKWFINNMITHFFGENTFTRFRLSHFPFTEPSFEIDIRCWLCQNGCSICKQTKWIEILGAGIIHPQVMNNMGIGDTENITGIAAGIGIERLAMLKYGIDDIRDFYDNNFKFLTQFTD" }, "paralog": false, "identity": 0.9941348973607038, "score": 681.0, "evalue": 2.11e-252, "description": "Internal stop codon at 231875, 232676. Nonsense mutation around 231875, 232676.", "psc": { "uniref90_id": "UniRef90_P47436", "gene": "pheS", "product": "phenylalanine--tRNA ligase subunit alpha", "ec_ids": [ "6.1.1.20" ], "uniref50_id": "UniRef50_P75564", "cog_id": "COG0016", "cog_category": "J", "kegg_orthology_id": "K01889", "go_ids": [ "GO:0000049", "GO:0000166", "GO:0000287", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47436", "EC:6.1.1.20", "UniRef:UniRef50_P75564", "COG:COG0016", "COG:J", "KEGG:K01889", "GO:0000049", "GO:0000166", "GO:0000287", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432" ] }, "pscc": { "uniref50_id": "UniRef50_P75564", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75564" ], "product": "Phenylalanine--tRNA ligase alpha subunit" } }, "id": "JPCNHPFPBM_457", "locus": "GCA000292505_00421" }, { "type": "cds", "sequence": "contig_1", "start": 232718, "stop": 232870, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01409.25" ], "nt": "ATGAATAACATGGGAATTGGGGATACTGAAAATATTACTGGGATAGCAGCAGGAATTGGGATTGAACGCTTAGCAATGTTAAAGTATGGGATTGATGATATCCGTGATTTTTATGATAACAACTTTAAGTTTTTAACCCAGTTTACTGACTAA", "aa": "MNNMGIGDTENITGIAAGIGIERLAMLKYGIDDIRDFYDNNFKFLTQFTD", "aa_hexdigest": "c10ad6a6cdb630eab0435e14e5630067", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01409.25", "name": "tRNA-synt_2d", "length": 47, "aa_cov": 0.94, "hmm_cov": 0.9574468085106383, "evalue": 6.892733919663018e-16, "score": 53.52961349487305, "start": 2, "stop": 48 } ], "seq_stats": { "molecular_weight": 5634.288600000001, "isoelectric_point": 4.293526649475097 }, "id": "JPCNHPFPBM_458", "locus": "GCA000292505_00422" }, { "type": "cds", "sequence": "contig_1", "start": 232874, "stop": 233347, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01588.26" ], "nt": "ATGTTGATATCAAAAAAAACACTTGGCGTTTTAATCCCTGATATCTTTAGTTTTTCTAATGATCAAATTGCCCAAAAGTTAGAACAAATGGGGATTGAAGTGGAATCAATTAAGCAGTTTAACAGCCCTGATTACCTCCAACTTGCAAAGGTTGTATCAATCCAACCCCATCCCCATGACAACAAGCTTTTTATCTGTGAATTACAAATTGATAAAAACAAGTTTATTAATGTTGTTTCCAATGCTAATAACATTAACAATCCTGATAATATCAACAAGTTTGTCATTGTTGCAAAAAAAGGAACTGAGTTACTCAACGGGTTAATTGTTAAAACCCAAAATATTAAAGGGATCATTTCAGAAGGGATTTTATGTAGCTATATTGACATTAACCCCTTCAGTAGACAGATCATTGAAAAAACAGAAGTTGCTGATGCGATTATCATTGATCATGTTAGCAATGATCATGACTGA", "aa": "MLISKKTLGVLIPDIFSFSNDQIAQKLEQMGIEVESIKQFNSPDYLQLAKVVSIQPHPHDNKLFICELQIDKNKFINVVSNANNINNPDNINKFVIVAKKGTELLNGLIVKTQNIKGIISEGILCSYIDINPFSRQIIEKTEVADAIIIDHVSNDHD", "aa_hexdigest": "0376e536e714d56cc3e409b342b40556", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01588.26", "name": "tRNA_bind", "length": 94, "aa_cov": 0.5987261146496815, "hmm_cov": 0.9148936170212766, "evalue": 8.540971889790484e-14, "score": 46.59867858886719, "start": 47, "stop": 140 } ], "seq_stats": { "molecular_weight": 17611.09349999999, "isoelectric_point": 5.525227546691894 }, "id": "JPCNHPFPBM_459", "locus": "GCA000292505_00423" }, { "type": "cds", "sequence": "contig_1", "start": 233381, "stop": 233608, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCTTTGATGTTAAAACCCCAACTAACAGAGCAGATCTTCATAGCTTAATCTTTTTAGCAAAAGAACTTGGGGTACTTTTGAAAACCAAAACCTTTTTAAAACAAAAAAGTAGTGTTGTTAACCATGACTTTTTTAAGTTTCCCCTAAATTTAAAAAACAAGTTAAAAGCGAATTATTTTGGCGGTTTGTTCTTAAGACAAATTAACCAACATAGTTCACCTTGA", "aa": "MIFDVKTPTNRADLHSLIFLAKELGVLLKTKTFLKQKSSVVNHDFFKFPLNLKNKLKANYFGGLFLRQINQHSSP", "aa_hexdigest": "c256bba9dff5f863cb54becac8b7c18a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8648.089899999997, "isoelectric_point": 10.237402153015136 }, "id": "JPCNHPFPBM_460", "locus": "GCA000292505_00424" }, { "type": "cds", "sequence": "contig_1", "start": 233636, "stop": 234280, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03483.22" ], "nt": "ATGATCAAACCAGTTAACTATTATGTTGATAAAGCTAACTTAGTAACAGTGTTCACCGCTCAGCCAATCCATTGTCATGATGCAGATAGAATTGTTGGTAACATTGAACTTAAACAAGCAACCCATAATGAAACTTTTGTTGGACTTGATGACAAGCAATATGAGATTGAACCAGGGGATATTGTTGTTTGTGATGAGAAGGGCATTATTGCACTGGTAGGGATCATTGGTTCAAAGCGCACAATGGTCCAACCTACAACAACTAACATCTTTTTTGAAGTTGTTAACTGTAACAGTGAAACCATTAAACAAACTGCCAAGCGCTTTTTGATCAATAACTTTGCCAGTAAGTTTATGGTTAAACAGATTAGCTTATTAGCTACTGATAACTGTTTAAACTACTTACAAAACAGTTTACTAACCACTGATAACATTGGCAAAATTAGCCACTTTTCAAGTTCGCTTAAAGTTGAACCATTTAGTAAAAAGCTCACAGTGAATTTCCATAAGATACGCCAACTAATTGGCATTGAAAAAAAGGAACTAACTGATCAAACCATTAAAAAAAGCCTCAGTCAACTAGGGTTTAAAGTTGACAACCAACTTCTCAAAATCCCCAGTTACAGACAAGACATTAATACTTGA", "aa": "MIKPVNYYVDKANLVTVFTAQPIHCHDADRIVGNIELKQATHNETFVGLDDKQYEIEPGDIVVCDEKGIIALVGIIGSKRTMVQPTTTNIFFEVVNCNSETIKQTAKRFLINNFASKFMVKQISLLATDNCLNYLQNSLLTTDNIGKISHFSSSLKVEPFSKKLTVNFHKIRQLIGIEKKELTDQTIKKSLSQLGFKVDNQLLKIPSYRQDINT", "aa_hexdigest": "b28f9bafe79e20a11bd7467f9a62c423", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03483.22", "name": "B3_4", "length": 140, "aa_cov": 0.6542056074766355, "hmm_cov": 1.0, "evalue": 1.816132869665551e-41, "score": 136.71591186523438, "start": 2, "stop": 141 } ], "seq_stats": { "molecular_weight": 24193.681700000023, "isoelectric_point": 8.787379264831543 }, "id": "JPCNHPFPBM_461", "locus": "GCA000292505_00425" }, { "type": "cds", "sequence": "contig_1", "start": 234302, "stop": 234685, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17759.6" ], "nt": "GTGAAGTTAATTGATATCAATAAGTTAAAACCAATTGGGATCACTAGTAGTTTTAACTTTGAAAAGTCTAGTTACTTTAACACTTTTAATGCTTTAACAAAACTAAGAAAAAAACTGCAAACACTTGGTTTTCACAACGTTATTACCTACCAGTTAACTGATCAAAAAAGTGCAAAAACTTTTAATTTGTTTAACTTAGAAAATTTCATCACCATTAAAAACCCAGTGTCCCAAAACCATTCTGTAATGCGTGTTAGCTTAATTGATTCACTGTTAAAAGTGCTACAAACCAATAACAACTATAAGAATGAACTGGTGAACATCTTTGAGTTTTCCTTTATTAAAACCCAAAACAATAGTGAACTGCACCTGGCAGTATTATGA", "aa": "MKLIDINKLKPIGITSSFNFEKSSYFNTFNALTKLRKKLQTLGFHNVITYQLTDQKSAKTFNLFNLENFITIKNPVSQNHSVMRVSLIDSLLKVLQTNNNYKNELVNIFEFSFIKTQNNSELHLAVL", "aa_hexdigest": "95ba04f1fd78526260de85933ef6a149", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17759.6", "name": "tRNA_synthFbeta", "length": 90, "aa_cov": 0.7007874015748031, "hmm_cov": 1.0, "evalue": 1.920183302552926e-21, "score": 71.59088134765625, "start": 30, "stop": 118 } ], "seq_stats": { "molecular_weight": 14717.84769999999, "isoelectric_point": 9.77703342437744 }, "id": "JPCNHPFPBM_462", "locus": "GCA000292505_00426" }, { "type": "cds", "sequence": "contig_1", "start": 234719, "stop": 235120, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17759.6" ], "nt": "ATGCAAAGGATAAGCAATGATGTTTTTACTATGAAGGGATTAGCAAAACTCATTGTTGCTAACTTAGGGTTTAGTTGTGACTTTGAACCACTTGATGATAGTGACTATTTTGTTAATAATCAAAGTTTAAAAATAGTAGTTTTTAACGAACAGATCGGTTTTATTGGGCTAATTAAAGAATCATTGTTAAATAACTATGATCTGAACAATAAACCCATTTATTGTCTTGAAATCAACTTAGATAGGATGCTCTCTTCTCTAAACAGGATTGAAAAAAACTACCTTGGTTACAGTAAACTACAACCTGTTTGCAAGGATCTTACCTTTAGTTTTACCAACCCTGCTAGTCACTTTGATCAGTTTGCTAACATGATCAAAAGGATAACTGGCATTGAAAGTTGA", "aa": "MQRISNDVFTMKGLAKLIVANLGFSCDFEPLDDSDYFVNNQSLKIVVFNEQIGFIGLIKESLLNNYDLNNKPIYCLEINLDRMLSSLNRIEKNYLGYSKLQPVCKDLTFSFTNPASHFDQFANMIKRITGIES", "aa_hexdigest": "1aac52b0e0dd170daf1aa2bcdfcdf35e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17759.6", "name": "tRNA_synthFbeta", "length": 76, "aa_cov": 0.5639097744360902, "hmm_cov": 0.9736842105263158, "evalue": 1.0112494137654471e-13, "score": 46.3557014465332, "start": 6, "stop": 80 } ], "seq_stats": { "molecular_weight": 15244.362699999983, "isoelectric_point": 5.315719032287597 }, "id": "JPCNHPFPBM_463", "locus": "GCA000292505_00427" }, { "type": "cds", "sequence": "contig_1", "start": 235145, "stop": 235294, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAACAACCAACTGATCACTAAGTACACCGTTCGTTATTTTCTGAAAAATGATGCTAACAAACCACTAACTAACCAAACAATTGAACTTATCACTAATAACTTAAAACTCCAGTGTGAAAAACTAAAAATTAAATTAGATATTTAG", "aa": "MQNNQLITKYTVRYFLKNDANKPLTNQTIELITNNLKLQCEKLKIKLDI", "aa_hexdigest": "758432b39fa1512bcb857ab864ede4d7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5822.796399999998, "isoelectric_point": 9.51832180023193 }, "id": "JPCNHPFPBM_464", "locus": "GCA000292505_00428" }, { "type": "cds", "sequence": "contig_1", "start": 235353, "stop": 235898, "strand": "+", "frame": 3, "gene": "infC", "product": "translation initiation factor IF-3", "db_xrefs": [ "COG:COG0290", "COG:J", "GO:0003743", "GO:0005829", "GO:0006413", "GO:0032790", "GO:0043022", "RefSeq:WP_014894620.1", "SO:0001217", "UniParc:UPI00027B3799", "UniRef:UniRef100_UPI00027B3799", "UniRef:UniRef50_P47438", "UniRef:UniRef90_P47438" ], "nt": "TTGGCACAAAACGCAAAACACCCTTCTAAAAAAGAACAAAAACCACTCGTTAATGAACAGATTGCTTTCAATCAGTTCACTTTAATTGATGAAAACAGTACTAATTTAGGGATAGTTAAGATGGAAAACGCTTTAAAGTTAGCACAAGAAAAACAGTTAGATCTAGTTCTAATTGCTCCAAACCCAACCAAACCGATCGTTAAGTTGTTGGACTTTGGCAGATATACCTATGATTTAAAGCGTAAGAAAAGACAAGCCAAGAAAAACCAAACAATCATCCAAACCAAAGAAGTTGTTGTCAAACCAACGATTGCTAAACATGATTTAGAATTTAGAGCAAAACAGAGTAAGAATTGGATAGAAAAAGGTCATCATGTCAAGTTTATAGTCCGTGCCTTTGGCAGGATTAGCACCAGGATAGAGTTAATTGAAAAGGTGTTTGATGACTTTTACCAGTTAGTTAAAGATGTAGTTGAGATCCAAAAACCTTTAACCGCTTCTTCCAAAACGATGTACGCTGCTCTATTAGTACCTTTAAAAAGATAG", "aa": "MAQNAKHPSKKEQKPLVNEQIAFNQFTLIDENSTNLGIVKMENALKLAQEKQLDLVLIAPNPTKPIVKLLDFGRYTYDLKRKKRQAKKNQTIIQTKEVVVKPTIAKHDLEFRAKQSKNWIEKGHHVKFIVRAFGRISTRIELIEKVFDDFYQLVKDVVEIQKPLTASSKTMYAALLVPLKR", "aa_hexdigest": "f74dab0cc21a36399546139fadb4086a", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3799", "ncbi_nrp_id": "WP_014894620.1", "uniref100_id": "UniRef100_UPI00027B3799", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3799", "RefSeq:WP_014894620.1", "UniRef:UniRef100_UPI00027B3799" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3799", "uniref90_id": "UniRef90_P47438" }, "psc": { "uniref90_id": "UniRef90_P47438", "gene": "infC", "product": "translation initiation factor IF-3", "uniref50_id": "UniRef50_P47438", "cog_id": "COG0290", "cog_category": "J", "go_ids": [ "GO:0003743", "GO:0005829", "GO:0006413", "GO:0032790", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_P47438", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47438" ], "product": "Translation initiation factor IF-3" }, "genes": [ "infC" ], "id": "JPCNHPFPBM_465", "locus": "GCA000292505_00429" }, { "type": "cds", "sequence": "contig_1", "start": 235901, "stop": 236080, "strand": "+", "frame": 2, "gene": "rpmI", "product": "50S ribosomal protein L35", "db_xrefs": [ "COG:COG0291", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904", "KEGG:K02916", "RefSeq:WP_009885738.1", "SO:0001217", "UniParc:UPI0000133FF7", "UniRef:UniRef100_P47439", "UniRef:UniRef50_P47439", "UniRef:UniRef90_P47439" ], "nt": "ATGAAAACCAAAAGTGCTGCAGTAAAACGCTTTAAACTCACCAAATCAGGACAAATTAAGCGCAAACACGCTTATACTTCCCACCTCGCGCCCCACAAATCAACCAAACAAAAGCGCCATTTGCGCAAGCAAGCTACTGTGAGCAACAGTGAATTGAAAAGAATTGGTATTTTAATTTAG", "aa": "MKTKSAAVKRFKLTKSGQIKRKHAYTSHLAPHKSTKQKRHLRKQATVSNSELKRIGILI", "aa_hexdigest": "a92c22b563615ebee0f1d0ca7619ae8f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133FF7", "ncbi_nrp_id": "WP_009885738.1", "uniref100_id": "UniRef100_P47439", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133FF7", "RefSeq:WP_009885738.1", "UniRef:UniRef100_P47439" ] }, "ips": { "uniref100_id": "UniRef100_P47439", "uniref90_id": "UniRef90_P47439" }, "psc": { "uniref90_id": "UniRef90_P47439", "gene": "rpmI", "product": "50S ribosomal protein L35", "uniref50_id": "UniRef50_P47439", "cog_id": "COG0291", "cog_category": "J", "kegg_orthology_id": "K02916", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P47439", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47439" ], "product": "Large ribosomal subunit protein bL35" }, "genes": [ "rpmI" ], "id": "JPCNHPFPBM_466", "locus": "GCA000292505_00430" }, { "type": "cds", "sequence": "contig_1", "start": 236083, "stop": 236457, "strand": "+", "frame": 1, "gene": "rplT", "product": "50S ribosomal protein L20", "db_xrefs": [ "COG:COG0292", "COG:J", "GO:0000027", "GO:0000315", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022625", "RefSeq:WP_009885739.1", "SO:0001217", "UniParc:UPI0000133D17", "UniRef:UniRef100_P47440", "UniRef:UniRef50_Q7NBC0", "UniRef:UniRef90_P47440" ], "nt": "ATGCGTGTTAAGGGAACAAATACAACCAGGATTAGAAGAAAAAAATGGTTAAAACAAGCTAGTGGTAGCTTTGGGACAAGAAAAGCTTCTTTTAAGGCAGCTAAACAAACTGTTATCCAAGCAAGCAAGTATGCTTACCGTGATAGGAGACAGAAAAAACGTGAGTTTCGTTCGTTGTGGATCTTAAGGTTAAATGCTGCACTGCGTGCACAAGGGATGACTTATTCAGTGTTTATCAATGAATTGAAAAAAGCCAAGATAGTCATTAACAGAAAGGTACTTTCTGAACTAGCAATTAAAGAACCTAATAAGTTAAATCTGATTATCAATACCATCAAAAAACCAACTAATAAACCAACTGTTGCAAAAACTTAG", "aa": "MRVKGTNTTRIRRKKWLKQASGSFGTRKASFKAAKQTVIQASKYAYRDRRQKKREFRSLWILRLNAALRAQGMTYSVFINELKKAKIVINRKVLSELAIKEPNKLNLIINTIKKPTNKPTVAKT", "aa_hexdigest": "7434c48886aa79e5163f3a2be45cec80", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133D17", "ncbi_nrp_id": "WP_009885739.1", "uniref100_id": "UniRef100_P47440", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133D17", "RefSeq:WP_009885739.1", "UniRef:UniRef100_P47440" ] }, "ips": { "uniref100_id": "UniRef100_P47440", "uniref90_id": "UniRef90_P47440" }, "psc": { "uniref90_id": "UniRef90_P47440", "gene": "rplT", "product": "50S ribosomal protein L20", "uniref50_id": "UniRef50_Q7NBC0", "cog_id": "COG0292", "cog_category": "J", "go_ids": [ "GO:0000027", "GO:0000315", "GO:0003735", "GO:0006412", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q7NBC0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q7NBC0" ], "product": "Large ribosomal subunit protein bL20" }, "genes": [ "rplT" ], "id": "JPCNHPFPBM_467", "locus": "GCA000292505_00431" }, { "type": "cds", "sequence": "contig_1", "start": 236429, "stop": 237136, "strand": "-", "frame": 3, "gene": "rnhC", "product": "Ribonuclease HIII", "db_xrefs": [ "COG:COG1039", "COG:L", "EC:3.1.26.4", "GO:0003723", "GO:0004523", "GO:0005737", "GO:0006298", "GO:0032299", "GO:0043137", "GO:0046872", "RefSeq:WP_009885740.1", "SO:0001217", "UniParc:UPI0000557D0F", "UniRef:UniRef100_P47441", "UniRef:UniRef50_P47441", "UniRef:UniRef90_P47441" ], "nt": "TTGCAACATTACAAACCAGCTGATTTTTATTTAATTGGCAGTGATGAATCTGGCAAGGGTGATAGTTTTGGTGGGATTTGTGTTAGTGCTGTTTTAATTGAAAAAAAACAGCTTATTAACCTTGAAAATTTACAAGTAACTGATTCAAAAAAACTCAGTGATCACACTGTCAAACTGTTAGCTAAATCGATCCAAACAACAGTAATGGATCACCATACAATTACATTAGATCCCAAGCAGTATAATGATTTGACAAAATCACTGAAAAATACCAATTTACTTTTGACCAATTTACACTGTCAGCTTTACCAAAAGTTACTGGAAAAAAATCAGTTATTAAGACAAACAGTAACAATTAGCATTGACCAGTTTGCCAATCAAGAGTTATTTGTTAACTATCTAAATAAGCTAACTAATTTCACTGATAAAACAGTGTTATTACCTGATCAGTTTCTAATTAATGGTGAAACTAAGTCACTGGTGATTGCAGCTGCATCTATCTTAGCAAGAGATAGCTTTATTAATCAGCTGCAATTACTCAACCAAAAGGTAAACTATGATTTACCCAAAGGCAGTAGTCATGGCATTGAACAAGCGTTGTTATTTCTTAACCAACAACGCGGTTTTAGTCAAATTGAACAGTTTAAACAGGTGGCAAAACTTAACTTTAAAAACGTTACTAAGTTTTTGCAACAGTTGGTTTATTAG", "aa": "MQHYKPADFYLIGSDESGKGDSFGGICVSAVLIEKKQLINLENLQVTDSKKLSDHTVKLLAKSIQTTVMDHHTITLDPKQYNDLTKSLKNTNLLLTNLHCQLYQKLLEKNQLLRQTVTISIDQFANQELFVNYLNKLTNFTDKTVLLPDQFLINGETKSLVIAAASILARDSFINQLQLLNQKVNYDLPKGSSHGIEQALLFLNQQRGFSQIEQFKQVAKLNFKNVTKFLQQLVY", "aa_hexdigest": "c2dc8a4dbeb8be236918658f2814e7a0", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000557D0F", "ncbi_nrp_id": "WP_009885740.1", "uniref100_id": "UniRef100_P47441", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000557D0F", "RefSeq:WP_009885740.1", "UniRef:UniRef100_P47441" ] }, "ips": { "uniref100_id": "UniRef100_P47441", "uniref90_id": "UniRef90_P47441" }, "psc": { "uniref90_id": "UniRef90_P47441", "gene": "rnhC", "product": "Ribonuclease HIII", "ec_ids": [ "3.1.26.4" ], "uniref50_id": "UniRef50_P47441", "cog_id": "COG1039", "cog_category": "L", "go_ids": [ "GO:0005737", "GO:0032299", "GO:0046872", "GO:0003723", "GO:0004523", "GO:0043137", "GO:0006298" ] }, "pscc": { "uniref50_id": "UniRef50_P47441", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47441" ], "product": "Ribonuclease HIII" }, "genes": [ "rnhC" ], "id": "JPCNHPFPBM_468", "locus": "GCA000292505_00432" }, { "type": "cds", "sequence": "contig_1", "start": 237183, "stop": 237578, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00226.36" ], "nt": "ATGGCTGAACAGAAACGTGATTATTATGAAGTGTTAGGAATAACCCCTGATGCTGATCAATCAGAGATTAAAAAAGCCTTTCGTAAGCTGGCTAAAAAGTACCATCCTGATCGTAACAACGCGCCTGATGCTGCTAAGATTTTTGCTGAAATTAATGAAGCAAATGATGTTTTATCAAACCCCAAAAAAAGAGCTAACTATGATAAGTATGGTTTTGATGGGGTTGATGGTGAACCTGCTTTTAACTTCCAAGCAGATGTTTTTCAATCCTTTTTTGAAGAGATAGCAAAATCAGGGGTTTTTAACAACCAAACCAATCCTGAACAAAAAGAAAAAAGAAACGTTACCACTGGTTCTCCAAAAAACCTAAGCAAGAACAACCTGAAATTAACTTAG", "aa": "MAEQKRDYYEVLGITPDADQSEIKKAFRKLAKKYHPDRNNAPDAAKIFAEINEANDVLSNPKKRANYDKYGFDGVDGEPAFNFQADVFQSFFEEIAKSGVFNNQTNPEQKEKRNVTTGSPKNLSKNNLKLT", "aa_hexdigest": "76ffdd33f98465c4b38c01e63809c587", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00226.36", "name": "DnaJ", "length": 63, "aa_cov": 0.4732824427480916, "hmm_cov": 1.0, "evalue": 9.00488983106079e-29, "score": 94.5582504272461, "start": 7, "stop": 68 } ], "seq_stats": { "molecular_weight": 14880.321499999993, "isoelectric_point": 7.841628074645997 }, "id": "JPCNHPFPBM_469", "locus": "GCA000292505_00433" }, { "type": "cds", "sequence": "contig_1", "start": 237608, "stop": 237748, "strand": "+", "frame": 2, "gene": null, "product": "DnaJ-like protein MG200", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002AA2D45", "UniRef:UniRef90_UPI0002AA2D45" ], "nt": "GTGCAACAAAACCAAAACCAAAACAAAGACCCAGATGAATTACGTTCTAAGGTCCCTGGAGAGGTTACTGCTAGTGATTGGGAAGCATTGGTTGGTGATACTAGGTATGGGTATTTTGATGAAACAGGGGATTGGAGTTGA", "aa": "MQQNQNQNKDPDELRSKVPGEVTASDWEALVGDTRYGYFDETGDWS", "aa_hexdigest": "daaead17ad282b9b33914626d5e66ce9", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 237551, "stop": 237805, "observations": { "mutation": [ 237746, 237782 ], "stop": [ 237746, 237782 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0002AA2D45", "query_cov": 1.0, "subject_cov": 0.5, "identity": 0.978, "score": 95.9, "evalue": 6.84e-25, "gene_start": 1, "gene_end": 46, "reference_start": 20, "reference_end": 65, "reference_sequence": "KQEQPEINLDHVVEQTIKKVQQNQNQNKDPDELRSKVPGEVTASDWEALVGDTRYGYFDETGDWSWKGYFDEQGKWVWNEPVDSLEHHHHHH" }, "paralog": false, "identity": 0.9761904761904762, "score": 146.0, "evalue": 1.56e-47, "description": "Internal stop codon at 237746, 237782. Nonsense mutation around 237746, 237782.", "psc": { "uniref90_id": "UniRef90_UPI0002AA2D45", "product": "DnaJ-like protein MG200", "uniref50_id": "UniRef50_UPI0002AA2D45", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0002AA2D45", "UniRef:UniRef50_UPI0002AA2D45" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0002AA2D45", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002AA2D45" ], "product": "DnaJ-like protein MG200" } }, "id": "JPCNHPFPBM_470", "locus": "GCA000292505_00434" }, { "type": "cds", "sequence": "contig_1", "start": 238022, "stop": 238987, "strand": "+", "frame": 2, "gene": "dnaJ", "product": "DnaJ C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002E59EF3", "UniRef:UniRef90_UPI0002E59EF3" ], "nt": "TTGTTAGATCTCAACCTCTTTACTATCCCAACTAAAGCTACTAAGGATGATCTTTTGTTTGACAACATTAACCTCACTACCTATGAACAAGTTGTTGATTATCTCAACAGTCAAGCAACCCCTAATTTAGCTAAAACCGATGGTGAATTGCAAACGATTGATGGTACCAACCCATTGTTATTAGAACAGTGCAAAAAGATCAAAAAACAAGCAGAACAACTCTTTAAAAAACTCTTTTTAAAAAAACAACTCCCCTTCATCACCCAACCTGAAGTTGTTGAGGAAAGTAAAACCAGTTTTGATGAGAACAACGTTAACCTTGTTTACTTTGAAAAGGTCCCTGAAATCCTTTTCATTAACCAACAACCTAAGGAGGTAAAATACACCCGTCAAGTCTTTGATGGGTTGACAAACAAAACAACTAGTGAAACGATTACACTAGAGATCCAACTACTCCAAACCCCAAAAGAGACTGTTAGTGCCATTTTTAAAGGCTTTGGTAATGACCATGGCAAGGGCTGTGGGGATTTAAAGATTGTTTTTGAAAAGATTAAAAGCCCCTTTTTTCAAGTCAATGAGGATGGCTTGCACTCTGCTTGCATCATTGACCCTTTAGTTGCTTACAACGGCGGGATTATCGATGTGTTTGGGCCCTACACTAACTTCCAAGTTAAGGTAGATGGGGAGATAGACATCAATGCCATTATGAAGTTTGAAAAACTAGGCATTGCTAAAACCAAACGCAAGGGCGATCTTTTTGTCCATCTCTATTACAGTAGTGTCCCTAAAAAGAAACTCACCACTAACCCCCAAGTTCAACAGTTCTTAGAACTTTTACAAGCTGAATATGAACTGTTGCAAGACAACATCAAGAGCTTAAAGTACTTTAAAAATAACCTAGTTATCCCCAAAAAGCCACTTGATCAACAAAGCTATCAATACCTCTCCCAAGAACCCATTAGTTAG", "aa": "MLDLNLFTIPTKATKDDLLFDNINLTTYEQVVDYLNSQATPNLAKTDGELQTIDGTNPLLLEQCKKIKKQAEQLFKKLFLKKQLPFITQPEVVEESKTSFDENNVNLVYFEKVPEILFINQQPKEVKYTRQVFDGLTNKTTSETITLEIQLLQTPKETVSAIFKGFGNDHGKGCGDLKIVFEKIKSPFFQVNEDGLHSACIIDPLVAYNGGIIDVFGPYTNFQVKVDGEIDINAIMKFEKLGIAKTKRKGDLFVHLYYSSVPKKKLTTNPQVQQFLELLQAEYELLQDNIKSLKYFKNNLVIPKKPLDQQSYQYLSQEPIS", "aa_hexdigest": "3cf905c80b63c1c53a1586ea2039436e", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0002E59EF3", "query_cov": 1.0, "subject_cov": 0.8941504178272981, "identity": 0.997, "score": 625.0, "evalue": 1.53e-225, "valid": true, "product": "DnaJ C-terminal domain-containing protein", "uniref50_id": "UniRef50_UPI0002E59EF3" }, "pscc": { "uniref50_id": "UniRef50_UPI0002E59EF3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002E59EF3" ], "product": "DnaJ C-terminal domain-containing protein" }, "genes": [ "dnaJ" ], "id": "JPCNHPFPBM_471", "locus": "GCA000292505_00435" }, { "type": "cds", "sequence": "contig_1", "start": 238999, "stop": 239451, "strand": "+", "frame": 1, "gene": "grpE", "product": "Protein GrpE", "db_xrefs": [ "COG:COG0576", "COG:O", "GO:0000774", "GO:0005737", "GO:0006457", "GO:0042803", "GO:0051082", "GO:0051087", "KEGG:K03687", "SO:0001217", "UniRef:UniRef50_P47443", "UniRef:UniRef90_P47443" ], "nt": "ATGTGTGAAAAATCACAAACAATTAAAGAGCTTTTAAACGCCATTAGAACCTTAGTTGTCAAGAACAATAAAGCTAAGGTTAGTATGATTGAAAAGGAACTGTTAGCTTTTGTTAGTGAACTTGACAAAAAGTTCAAACAACAACTCAACAACTTCAATGAACTACAACAAAAGATCCCACTACTCCAAAAAGCTAACGAAGAGTTTGCTTTAAAGTTTGAAAGGATGCAACGCGAAGCACAAAACCAGATCCAAGCCAAACTAGATGAGTTGAATCTTAAAAATAAAAAGGAGTTAGAACAAGCCAAGAAATATGCGATTGCCAAAACCCTTGACCAACCCTTAAACATCATCGATCAGTTTGAAATCGCGCTTTCATATGCCCAAAAAGACCCTCAAGTAAAAAACTATACCACTGGTTTTACCATGGTACTTGATGCTTTTTCAAGGTGA", "aa": "MCEKSQTIKELLNAIRTLVVKNNKAKVSMIEKELLAFVSELDKKFKQQLNNFNELQQKIPLLQKANEEFALKFERMQREAQNQIQAKLDELNLKNKKELEQAKKYAIAKTLDQPLNIIDQFEIALSYAQKDPQVKNYTTGFTMVLDAFSR", "aa_hexdigest": "dcd1c5028af2b4cc9c5a6cc785425d1b", "start_type": "ATG", "rbs_motif": null, "genes": [ "grpE" ], "truncated": "3-prime", "pseudogene": { "start": 238999, "stop": 239652, "observations": { "mutation": [ 239449 ], "stop": [ 239449 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47443", "query_cov": 1.0, "subject_cov": 0.6912442396313364, "identity": 1.0, "score": 275.0, "evalue": 4.01e-92, "gene_start": 1, "gene_end": 150, "reference_start": 1, "reference_end": 150, "reference_sequence": "MCEKSQTIKELLNAIRTLVVKNNKAKVSMIEKELLAFVSELDKKFKQQLNNFNELQQKIPLLQKANEEFALKFERMQREAQNQIQAKLDELNLKNKKELEQAKKYAIAKTLDQPLNIIDQFEIALSYAQKDPQVKNYTTGFTMVLDAFSRWLEANGVTKIKIEPGMEFDEKIMSALELVDSNLAKNKVVRVSKSGYKLYDKVIRFASVFVSKGNKKS" }, "paralog": false, "identity": 0.9953917050691244, "score": 412.0, "evalue": 7.66e-149, "description": "Internal stop codon at 239449. Nonsense mutation around 239449.", "psc": { "uniref90_id": "UniRef90_P47443", "gene": "grpE", "product": "Protein GrpE", "uniref50_id": "UniRef50_P47443", "cog_id": "COG0576", "cog_category": "O", "kegg_orthology_id": "K03687", "go_ids": [ "GO:0005737", "GO:0000774", "GO:0042803", "GO:0051087", "GO:0051082", "GO:0006457" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47443", "UniRef:UniRef50_P47443", "COG:COG0576", "COG:O", "KEGG:K03687", "GO:0005737", "GO:0000774", "GO:0042803", "GO:0051087", "GO:0051082", "GO:0006457" ] }, "pscc": { "uniref50_id": "UniRef50_P47443", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47443" ], "product": "Protein GrpE" } }, "id": "JPCNHPFPBM_472", "locus": "GCA000292505_00436" }, { "type": "cds", "sequence": "contig_1", "start": 239452, "stop": 239652, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAGCAAATGGGGTTACCAAGATTAAGATTGAACCAGGGATGGAATTTGATGAAAAGATTATGTCTGCATTGGAACTAGTTGATTCTAACCTTGCTAAAAACAAGGTAGTAAGAGTCTCAAAATCTGGCTATAAACTCTATGACAAAGTGATCCGCTTTGCATCAGTATTTGTCAGCAAAGGTAATAAAAAATCATAA", "aa": "MEANGVTKIKIEPGMEFDEKIMSALELVDSNLAKNKVVRVSKSGYKLYDKVIRFASVFVSKGNKKS", "aa_hexdigest": "e587f55f73e988ad5231e8e176511e67", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7367.566200000002, "isoelectric_point": 9.646807289123537 }, "id": "JPCNHPFPBM_473", "locus": "GCA000292505_00437" }, { "type": "cds", "sequence": "contig_1", "start": 239662, "stop": 240030, "strand": "+", "frame": 1, "gene": null, "product": "MPN121 family protein", "db_xrefs": [ "RefSeq:WP_010869370.1", "SO:0001217", "UniParc:UPI0000139460", "UniRef:UniRef100_P47444", "UniRef:UniRef50_P75353", "UniRef:UniRef90_P47444" ], "nt": "ATGAGTGAACAAAAAAGAAGAACAATCCAAATTGCGATAAGTGAAGACCACTATGAAGAGTTACAAAAGGCATTGGAACTACTTAAAGGGACCCAATTACCCTTTTCAACCACTGTTGAACAGTTTGTGGAGTTAATCTTATCTAACTATGTAGCTACTTCCAATAAGATTAGTAGTTTAGCTAAGAGTGGTTTTGATGTAGCTTCATTGCAGCAAGAACTTGAGAAGATAGGTAACCTTAGTGGGGTTGATGATAACCTCAAGGGTTTTCTCTCAGAACTGTTGAAAACCTCAAGGAATGGGTTTAGTAACCCCAATAAAGATGGCAAAAAAAATGATGACGATAATAACTCGTCATCAAAATCATAG", "aa": "MSEQKRRTIQIAISEDHYEELQKALELLKGTQLPFSTTVEQFVELILSNYVATSNKISSLAKSGFDVASLQQELEKIGNLSGVDDNLKGFLSELLKTSRNGFSNPNKDGKKNDDDNNSSSKS", "aa_hexdigest": "864cae9723138c15b3bad2d2b444adbb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139460", "ncbi_nrp_id": "WP_010869370.1", "uniref100_id": "UniRef100_P47444", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139460", "RefSeq:WP_010869370.1", "UniRef:UniRef100_P47444" ] }, "ips": { "uniref100_id": "UniRef100_P47444", "uniref90_id": "UniRef90_P47444" }, "psc": { "uniref90_id": "UniRef90_P47444", "product": "MPN121 family protein", "uniref50_id": "UniRef50_P75353" }, "pscc": { "uniref50_id": "UniRef50_P75353", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75353" ], "product": "Uncharacterized protein MG202 homolog" }, "genes": [], "id": "JPCNHPFPBM_474", "locus": "GCA000292505_00438" }, { "type": "tRNA", "sequence": "contig_1", "start": 240048, "stop": 240121, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(ggt)", "amino_acid": "Thr", "anti_codon": "ggt", "score": 61.8, "nt": "GCCGAATTGGTTTAGGGGCAAAACAGATCCATGGTAAGGATCAGAGAACAGTTCGACTCTGTTATTCGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 240111, 240113 ], "id": "JPCNHPFPBM_33", "locus": "GCA000292505_00439" }, { "type": "cds", "sequence": "contig_1", "start": 240158, "stop": 241903, "strand": "+", "frame": 2, "gene": "parE", "product": "DNA topoisomerase IV subunit B", "db_xrefs": [ "COG:COG0187", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0006265", "GO:0034335", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47445", "UniRef:UniRef90_P47445" ], "nt": "ATGAAAAGTAACTACAGTGCAACTAACATCAAGATCTTAAAGGGTTTGGATGCAGTTAAAAAGCGTCCGGGGATGTACATTGGTTCTACTGATAGTAAGGGTCTGCACCACATGCTATGGGAAATTCTTGCTAACAGTGTTGATGAAGTTTTAGCTGGTTATGCAACCAATATTACTGTTACTTTAGATCTCAACAACACCATTACTGTTAGTGATGATGGCAGGGGTATTCCCTATGAGATCCACCAAGACAGTAACATCTCTACGATCGATACAGTTTTCACCTTTCTCCATGCAGGGGGGAAGTTTGATGATCAGTCATACAAACTAGCAGGGGGATTACATGGAGTTGGTGCATCAGTGGTCAATGCCTTAAGTGATCATTTAGAAGTAACAGTGAAAAGAAATGGTCAGATCTACCAATCAGTTTATCAAGCTGGGGGTAAGATCATCCAAAAAGCCAAAAAGATTGGTGATACAACTAGCCATGGTACCACTGTTAGTTTCCATGCTGACCCTAAGGTCTTTAAAAAGGCTCAATTTGATAGCAACATTATTAAAAGCAGGTTAAAAGAGCTAAGCTTTCTGTTTGCTAAACTAAAGCTCACTTTTACTGATCAAAAAACTAATAAAACCACTGTTTTTTTTAGTACCTCAGGACTAGTTCAGTTCCTTGATGAAATTAATAATACTGTAGAAACACTTGGCCAAAAAACACTGATTAAAGGTGAGAAGGATGGGATTGAAGTGGAAGTGGTTTTCCAGTTTAACCAATCAGATCAAGAGACAATCTTATCATTTGCTAACTCGATTAAAACCTTTGAAGGAGGGAGTCATGAAAATGGGTTTTGTCTTGCCATTAGTGATGTGATCAACAGCTATTGCAGAAAGTACAACTTACTAAAAGAAAAAGATAAAAACTTTCAACTTAGTGAGATCAGACAAGGGTTGAATGCTATTATCAAAGTTAACTTACCTGAAAAAAACATCGCTTTTGAAGGACAAACTAAGAGTAAGTTGTTTTCAAAGGAAGTGAAAAACGTTGTTTATGAATTGGTCCAACAACACTATTTCCAGTTTCTGGAAAGAAACAACAATGATGCTAAATTGATCATTGATAAACTACTCAATGCTAGAAAGATTAAAGAGCAAATCAAACAACAACGTGAGTTGAAAAAAAGTTTATCAAGTCCCCAAAAAGAGAAGATCTTATTTGGGAAGTTAGCACCTTGTCAAACCAAAAAAACCAGTGAAAAAGAGTTGTTTATTGTTGAAGGTGATAGTGCTGGTGGCACTGCTAAAATGGGCCGTGATAGAATTTTTCAAGCTATCTTACCTTTGCGCGGCAAGGTGTTAAATGTTGAAAAAATTAACAATAAGAAGGAAGCGATCACTAACGAAGAGATCCTCACTTTAATCTTTTGTATTGGTACAGGGATTTTAACTAACTTCAACATCAAGGACTTAAAGTACGGAAAGATCATCATTATGACTGATGCAGATAATGATGGCGCACACATCCAAATCCTCTTACTTACCTTCTTTTATAGGTACATGCAACCCCTAATTGAACTGGGCCATGTCTATCTAGCTCTTCCTCCTTTATATAAGCTAGAAACCAAAGATAGAAAAACAGTTAAATACCTCTGGAGTGATTTGGAGTTGGAATCAGTCAAACTAAAGCTTAATAACTTCACTTTACAACGATACAAAGGACTTGGGGAGATGAATGCTGATCAGTTGTGA", "aa": "MKSNYSATNIKILKGLDAVKKRPGMYIGSTDSKGLHHMLWEILANSVDEVLAGYATNITVTLDLNNTITVSDDGRGIPYEIHQDSNISTIDTVFTFLHAGGKFDDQSYKLAGGLHGVGASVVNALSDHLEVTVKRNGQIYQSVYQAGGKIIQKAKKIGDTTSHGTTVSFHADPKVFKKAQFDSNIIKSRLKELSFLFAKLKLTFTDQKTNKTTVFFSTSGLVQFLDEINNTVETLGQKTLIKGEKDGIEVEVVFQFNQSDQETILSFANSIKTFEGGSHENGFCLAISDVINSYCRKYNLLKEKDKNFQLSEIRQGLNAIIKVNLPEKNIAFEGQTKSKLFSKEVKNVVYELVQQHYFQFLERNNNDAKLIIDKLLNARKIKEQIKQQRELKKSLSSPQKEKILFGKLAPCQTKKTSEKELFIVEGDSAGGTAKMGRDRIFQAILPLRGKVLNVEKINNKKEAITNEEILTLIFCIGTGILTNFNIKDLKYGKIIIMTDADNDGAHIQILLLTFFYRYMQPLIELGHVYLALPPLYKLETKDRKTVKYLWSDLELESVKLKLNNFTLQRYKGLGEMNADQL", "aa_hexdigest": "277bb6ee9e7f5a712c22257600721f8c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47445", "query_cov": 1.0, "subject_cov": 0.9178515007898894, "identity": 1.0, "score": 1124.0, "evalue": 0.0, "valid": true, "gene": "parE", "product": "DNA topoisomerase IV subunit B", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P47445", "cog_id": "COG0187", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0006265", "GO:0034335", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47445", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47445" ], "product": "DNA topoisomerase 4 subunit B" }, "genes": [ "parE" ], "id": "JPCNHPFPBM_475", "locus": "GCA000292505_00440" }, { "type": "cds", "sequence": "contig_1", "start": 241913, "stop": 242059, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCCAACTACCAGAAAGCTAGTGCAAGTAAAGCTTGATGATCTAATTAACGCTGAAAAGCAAATCAACATCTTTATGGGTGAAAAGAGTGATTTGCGCAAACACTGGATTGAAGCCAACATTAACTTTAGTGTGGAAAACTAA", "aa": "MNPTTRKLVQVKLDDLINAEKQINIFMGEKSDLRKHWIEANINFSVEN", "aa_hexdigest": "028fb2ef8b90c14f4f361c9eeedc31c4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5642.425299999999, "isoelectric_point": 6.539689826965333 }, "id": "JPCNHPFPBM_476", "locus": "GCA000292505_00441" }, { "type": "cds", "sequence": "contig_1", "start": 242059, "stop": 242346, "strand": "+", "frame": 1, "gene": null, "product": "DNA gyrase subunit A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9XCU2" ], "nt": "ATGGATCAAAAAAACAACAACCTCTTTCAAAAGGCAATTGAAGAAGTCTTTGCAGTTAGCTTTAGTAAGTATGCTAAATACATCATCCAAGATAGAGCTTTACCTGATCTAAGAGATGGGTTAAAACCAGTACAAAGACGGATCTTATATGGGATGTTTCAAATGGGCTTAAAACCCACCACTCCCTATAAAAAATCAGCCCGTGCTGTTGGGGAGATCATGGGGAAATACCACCCCCATGGTGATAGTTCCATTTATGATGCAATTATCAGAATGTCCCAAAGCTGA", "aa": "MDQKNNNLFQKAIEEVFAVSFSKYAKYIIQDRALPDLRDGLKPVQRRILYGMFQMGLKPTTPYKKSARAVGEIMGKYHPHGDSSIYDAIIRMSQS", "aa_hexdigest": "3c5d2d43d1ff1f3ef34dfe1c73e2937b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028540D82", "query_cov": 0.8736842105263158, "subject_cov": 0.83, "identity": 0.6509999999999999, "score": 122.0, "evalue": 1.42e-34, "valid": false, "product": "DNA gyrase subunit A", "uniref50_id": "UniRef50_Q9XCU2" }, "pscc": { "uniref50_id": "UniRef50_Q9XCU2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9XCU2" ], "product": "DNA gyrase subunit A" }, "genes": [], "id": "JPCNHPFPBM_477", "locus": "GCA000292505_00442" }, { "type": "cds", "sequence": "contig_1", "start": 242392, "stop": 244404, "strand": "+", "frame": 1, "gene": "parC", "product": "DNA topoisomerase IV subunit A", "db_xrefs": [ "COG:COG0188", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0005886", "GO:0006265", "GO:0009330", "GO:0034335", "SO:0001217", "UniRef:UniRef50_P47446", "UniRef:UniRef90_P47446" ], "nt": "GTGGATGGGGATAATGCTGCAGCAATGCGTTACACAGAAACCCGCTTAAGCTTGTATGGATTTGAACTATTAAAAGACATTGATAAAAAGTTAGTTAGTTTTATCAATAACTTTGATGATAGTGAAAAAGAACCAACGGTTTTACCAACCTTACTGCCTAACCTCTTTATCAATGGTGCGAGTGGGATAGCTGCTGGATATGCAACTAATATTGCTCCCCATAACACTAATGAACTATTAGATAGTCTTTGCTTGCGAATAGACCAACCTGATTGTGAACTTAAACAAATTTTAAAAATTGTTAAAGGTCCTGATTTTCCAACAGGGGGTAATGTTTATTTTGAAAAGAGTTTAAGTGATATTTATCAAGCAGGCAAAGGTAAATTTATTATCCAAGCTAAGTATGAAGTTAACAAGAACTTAAACCAGATTGAAATTACCCAAATCCCTTATGAAACACTGAAAGCTAACATTGTCAAACAAATTGAAGAGATTATCTTTGACAATAAACTATCTGCTATTGAAAGTGTCATTGATAGTTCAGATCGCAACGGCATTAGGATCATTATTAAACACAAGGACTTTTTGCCTGCTGAGAAGATCATGGCCTTTTTGTTTAAACACACCCAACTCCAAGTGAACTTTAACCTTAATAACACCGTGATTGCTAACCGCTTTCCCATCCAAATTGGTTTACTAAGTTACCTCGATCATTTTTTAAAGTTTTGTCATGAACTAATTATTAATAAAGCTAAGTATGAACTTGAGCTTGCAAGCAAGCGCTTGGAAATTATTTTAGGACTAATTAAAGCGATTAGTATCATTGATAAAATCATCAAATTAATTAGATCAGCAGTTGACAAAAGTGATGCAAGAGAAAAGTTAATTGATAACTTTAAATTTACTTTTAACCAAGCAGAGGCAATTGTTAGTTTGCGACTTTACCAACTAACTAACACTGATATTTTTGAACTTAACCAAGAACAAAATGAACTTGAAAAAACTGTGATTAGTTCAAAGCAACTAATTGCTAGTGAAAAAGCAAGAAACAAACTCCTAAAAAAACAGTTTGAAGGTTATAAAAAGCAGTTTCACCAGCAACGAAGGTCACAAATATGTGGCTTTATTAACCAAAAAAAGGTGGAGGAAAGTGAGCTAATTGAAAACAAAACTTATGGGGTTTTAATCACTAAAGCTGGTAACTACCATAAGTTTGAATCTAACCAACTATTAAAAAGCACCACTGATTTTAAAAGTGAGAGTGACACAATTATCTTTGCACAAACTATTGCTAATACCGACCAAATTTTTATTGTCACTTCACTAGGTAACATTATTAATATCCCTGTTTATAAATTAGCTTTCAATTCCAAAAATAAACTAGCAAGTTTAGTTAGTAAAAAACCAATCCTTTTGGAGTATGAAACGATTGTTTTTGTTGGAACAATGAACAGTGTAAACCAACCAATCCTTGTTTTAACTTCCAAACTAGGAATGGTTAAACGGATTGATTTAACCAAACTTAACAATAAGCCACTTAAAGCTACTTTGTGTATCTCACTCCGTGATAAAGACCAGTTAGTAAGTGCATTTTTACAACAAGATGATAAACTGATCTGTTTAGTGTCTGATCACAACTATTACACAGTTTTTCACACCAATGAGATCCCACTAATTAGTAGTAAGGGGATGGGAGTGAAGGGGATGAAGTTAAAACTAGAGGATCAAATTAAGTTTGTTGTTGCTTTTGAAGCTAATGAACCGTTAGTGATGATATGTAGTGATGGTAGTGTCATTAACTTAAAACAAACTGAACTAGTTGTAGTTAGCAGGATGGCAACTACAAAAAAACTGCCTGTTAAGAAAGCAATTAACTATTGTTTTAGTGATGCAACTAACACCCAGTTAATTAATTTTCAGGGTAAGAACGGTAGTAAATTAATTACAACTAGTGAACTGAACCAGATGAGTAAAACTGCAATTAGTCAAACCAGGTTTAACAAACTTAATTAG", "aa": "MDGDNAAAMRYTETRLSLYGFELLKDIDKKLVSFINNFDDSEKEPTVLPTLLPNLFINGASGIAAGYATNIAPHNTNELLDSLCLRIDQPDCELKQILKIVKGPDFPTGGNVYFEKSLSDIYQAGKGKFIIQAKYEVNKNLNQIEITQIPYETLKANIVKQIEEIIFDNKLSAIESVIDSSDRNGIRIIIKHKDFLPAEKIMAFLFKHTQLQVNFNLNNTVIANRFPIQIGLLSYLDHFLKFCHELIINKAKYELELASKRLEIILGLIKAISIIDKIIKLIRSAVDKSDAREKLIDNFKFTFNQAEAIVSLRLYQLTNTDIFELNQEQNELEKTVISSKQLIASEKARNKLLKKQFEGYKKQFHQQRRSQICGFINQKKVEESELIENKTYGVLITKAGNYHKFESNQLLKSTTDFKSESDTIIFAQTIANTDQIFIVTSLGNIINIPVYKLAFNSKNKLASLVSKKPILLEYETIVFVGTMNSVNQPILVLTSKLGMVKRIDLTKLNNKPLKATLCISLRDKDQLVSAFLQQDDKLICLVSDHNYYTVFHTNEIPLISSKGMGVKGMKLKLEDQIKFVVAFEANEPLVMICSDGSVINLKQTELVVVSRMATTKKLPVKKAINYCFSDATNTQLINFQGKNGSKLITTSELNQMSKTAISQTRFNKLN", "aa_hexdigest": "c9d2a76ab63fd90e997b9781f686b2fa", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47446", "query_cov": 1.0, "subject_cov": 0.8578745198463509, "identity": 0.991, "score": 1238.0, "evalue": 0.0, "valid": true, "gene": "parC", "product": "DNA topoisomerase IV subunit A", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P47446", "cog_id": "COG0188", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003918", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0005886", "GO:0006265", "GO:0009330", "GO:0034335" ] }, "pscc": { "uniref50_id": "UniRef50_P47446", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47446" ], "product": "DNA topoisomerase 4 subunit A" }, "genes": [ "parC" ], "id": "JPCNHPFPBM_478", "locus": "GCA000292505_00443" }, { "type": "cds", "sequence": "contig_1", "start": 244401, "stop": 244631, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCATTCATAAACCAAACCAACCAAAAGACCAACATTGTCTTTGGTGATCAATTGGGTTTTAAAGAGATATCAGTTGCTTCTACTTTAATCAATACCACCAGTGAAGCCAAGCACCAACTTGCTATAGTAGGACCAACCAGAATGGACTACCAAAAGATTAAAGCCTTATTAACTACTCTAAAAGAAGAGATAGAAAAATATGATAAAAAGATCCACAACCAAACCTAA", "aa": "MAFINQTNQKTNIVFGDQLGFKEISVASTLINTTSEAKHQLAIVGPTRMDYQKIKALLTTLKEEIEKYDKKIHNQT", "aa_hexdigest": "09cf3b39302cca20f7123ef5ddd3eaaa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8638.835499999997, "isoelectric_point": 9.005153465270997 }, "id": "JPCNHPFPBM_479", "locus": "GCA000292505_00444" }, { "type": "cds", "sequence": "contig_1", "start": 244638, "stop": 245432, "strand": "-", "frame": 2, "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "db_xrefs": [ "COG:COG1420", "COG:K", "GO:0003677", "GO:0006355", "GO:0045892", "KEGG:K03705", "SO:0001217", "UniRef:UniRef50_P75351", "UniRef:UniRef90_P47447" ], "nt": "ATGAAGAATTTAACGCCACGGCAAGCCCAAATTCTCAAAGCAATTATCAATGAATACATTGCTTATGCTATCCCTGTTGGCAGTAAATTACTAACCAAAAAATATTTTAAAAACCTCTCTGGGGGAACATTACGAAATGAGATGGCAGCTTTAGAGAAAAAAGGGTTCTTAAAGAAAAACCACATCTCTTCAGGCCGTGTTCCTTCCCAAATTGGTTATCAGTATTATGTCAAAGTGTTAAATGTTAGTAACACCACTAACGATCTGAAAACCCGCTTAAGAAGTGTTATTCTCCAGCAACACAAAACGATTGATGAGGTGATTGAACTTGGGGTTAAGTTTATCAATGAGATCATTAACCTACCAGTAGTGTTAACTAACTTTAGTAGTGATGAAGTTTTAAAAAAGATTGATCTGATCATCCTTGATAAGTCATTTGCCTTATTTTTATTGGTCTCCGCTAGTGGTAAAGTCTTTAAAAAAACCATTAGCTATGCTAACCAAAGGCAGTTTGAAGACATTGTTATTTGTGTAAGGATCTTTAATGATCGCATCATTGATACCCGCTTTTCAGAGATTAACAACCAACTAGAAGTTTTAAAAGAGATCATTAGAACCAAGGTTCATGAGTATCAGTATGTAATTGATGAGATCTTATTTAAGTTATTTGATTTAGATCAAATTGAGGCAAATAAAAAGATCTATGGGATCCAATACCTTGCTAAACAACCCGAGTTTGCTAACCAAGAGAAACTCACTAAAATCTTAAATCTGCTTGAAGATACTAGTGTTTGA", "aa": "MKNLTPRQAQILKAIINEYIAYAIPVGSKLLTKKYFKNLSGGTLRNEMAALEKKGFLKKNHISSGRVPSQIGYQYYVKVLNVSNTTNDLKTRLRSVILQQHKTIDEVIELGVKFINEIINLPVVLTNFSSDEVLKKIDLIILDKSFALFLLVSASGKVFKKTISYANQRQFEDIVICVRIFNDRIIDTRFSEINNQLEVLKEIIRTKVHEYQYVIDEILFKLFDLDQIEANKKIYGIQYLAKQPEFANQEKLTKILNLLEDTSV", "aa_hexdigest": "681cc5b1d1a7ea27d7e8294c69d49085", "start_type": "ATG", "rbs_motif": null, "genes": [ "hrcA" ], "truncated": "5-prime", "pseudogene": { "start": 244401, "stop": 245432, "observations": { "mutation": [ 244640 ], "stop": [ 244640 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47447", "query_cov": 1.0, "subject_cov": 0.7696793002915452, "identity": 1.0, "score": 503.0, "evalue": 1.68e-178, "gene_start": 1, "gene_end": 264, "reference_start": 1, "reference_end": 264, "reference_sequence": "MKNLTPRQAQILKAIINEYIAYAIPVGSKLLTKKYFKNLSGGTLRNEMAALEKKGFLKKNHISSGRVPSQIGYQYYVKVLNVSNTTNDLKTRLRSVILQQHKTIDEVIELGVKFINEIINLPVVLTNFSSDEVLKKIDLIILDKSFALFLLVSASGKVFKKTISYANQRQFEDIVICVRIFNDRIIDTRFSEINNQLEVLKEIIRTKVHEYQYVIDEILFKLFDLDQIEANKKIYGIQYLAKQPEFANQEKLTKILNLLEDTSVWQQMAFINQTNQKTNIVFGDQLGFKEISVASTLINTTSEAKHQLAIVGPTRMDYQKIKALLTTLKEEIEKYDKKIHNQT" }, "paralog": false, "identity": 0.9970845481049563, "score": 657.0, "evalue": 6.72e-242, "description": "Internal stop codon at 244640. Nonsense mutation around 244640.", "psc": { "uniref90_id": "UniRef90_P47447", "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "uniref50_id": "UniRef50_P75351", "cog_id": "COG1420", "cog_category": "K", "kegg_orthology_id": "K03705", "go_ids": [ "GO:0003677", "GO:0006355", "GO:0045892" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47447", "UniRef:UniRef50_P75351", "COG:COG1420", "COG:K", "KEGG:K03705", "GO:0003677", "GO:0006355", "GO:0045892" ] }, "pscc": { "uniref50_id": "UniRef50_P75351", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75351" ], "product": "Heat-inducible transcription repressor HrcA" } }, "id": "JPCNHPFPBM_480", "locus": "GCA000292505_00445" }, { "type": "cds", "sequence": "contig_1", "start": 245801, "stop": 247261, "strand": "+", "frame": 2, "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "db_xrefs": [ "COG:COG0322", "COG:L", "GO:0003677", "GO:0005737", "GO:0006289", "GO:0006974", "GO:0009380", "GO:0009381", "GO:0009432", "KEGG:K03703", "SO:0001217", "UniRef:UniRef50_P47448", "UniRef:UniRef90_P47448" ], "nt": "TTGGAATTAGCCAGAAAATACCAGATTAAAACCACTAAGTGCTTTGGACCGTTTGCTTCAAGTAAGTTTAAGTTACGTGAGATCCATGACCTACTTTTAAAACTCTTTCCTTTAAGAAAGTGTGCACCTCACCAAAAAAACCACCCGTGCTTTTATTTTCAGATGGGTTTATGTATGGGGCAATGTATGCAAACTGATACTAAGGAAAAATACCAACAGGTAATTAGTAACATTGAACAGTTTTTTAATGACCCTAGTGTGGTAATTAACTATTTAAAAGCTGCAGAAAAAAAGGCAAGTGATAATCAGGAATTTGAAAAGGCCCAGCAGTTTCTAACACTGCAAAAAGCAGTTTTAGAGTTAACAAAAACCCACCATACCACTATCATTAAACAAAAATCAAGCCATGATTTTATTGGGTATGTCTTTCAAAATAACGTTTTGGCCATTACCATTTTTTGTTATGAAAAAGGGGAGTTAACTGATAAAGAACAAGCAGTGTTTACCCTAGAGCAAACTGACATTGTGGAAGTTGAAAGTGCTATTATCACCTTTATCTACCACCACTATAAAACTACCCCACTTCCAAGTAAGATTACTGTTTCACTTGATGAAACTAACCTAAAACTTATTAGTGATAGCTTAAAAATTGGTGTTTTTAAGCCCAAGGATGGTAATGAAAAACTGATCTTACAAACTGTTATTGATAATGCCAAACATGCACTTGCAACCAAGTGGTTGAAGTTTACTAGTAACTATGATAAAACCCAGCTCCACAAGGATTTAGCACAACTTCTAAATACTGATTATATCCATAGTCTTGAGATTATTGATGTGTCATTCTATGATCAAAACCATGTTGTTGGTTGCATGTTAAGGTTTGAAGATGGTAAAAAGATCAAACACTTATCAAGAAGATACAACATTAACAGTTTAAAAAAAGGTGATACTAACCACATTGCTTTACTTGTTTACAGAAGGATCTTAAGTGCGATGCAAACCAAAGCTAACCTCCCTTTTAGTGATCTTTTAATTATTGATGGTGGTAAAGCACAAATTAAAAGTGTTAAGCAAGTTTTTAGTCTCTTCAGTAATGTTAAACCACCCATTATCATTGGACTAGTTAAAAACAAAAACCACCAAACTGATCACATTATGTTATCTGATTTCCAAGTTAAAAAGATAGCAATTAACTCCCCACTCTTTCACTATTTAGCAACAATCCAAACTGAAGTTGATGGTTTTGCTAAAAGAAGTGCTTTTAATAAGTTAAGTAACCACCAACTGCAAAACCCGTTGCTACAAATCCCAGGAGTTGGCAAGATAACTGCCCAAATTCTCTTTGATAACTTTCAAACGCTCAATAACATAAAATTAGCTTCAGTTAATGAGTTAAGCCAGTTTATTAAAAAACCATTAGCACAAAAGATTAAAACTTACTTTGCAAAACAAACTGATTAA", "aa": "MELARKYQIKTTKCFGPFASSKFKLREIHDLLLKLFPLRKCAPHQKNHPCFYFQMGLCMGQCMQTDTKEKYQQVISNIEQFFNDPSVVINYLKAAEKKASDNQEFEKAQQFLTLQKAVLELTKTHHTTIIKQKSSHDFIGYVFQNNVLAITIFCYEKGELTDKEQAVFTLEQTDIVEVESAIITFIYHHYKTTPLPSKITVSLDETNLKLISDSLKIGVFKPKDGNEKLILQTVIDNAKHALATKWLKFTSNYDKTQLHKDLAQLLNTDYIHSLEIIDVSFYDQNHVVGCMLRFEDGKKIKHLSRRYNINSLKKGDTNHIALLVYRRILSAMQTKANLPFSDLLIIDGGKAQIKSVKQVFSLFSNVKPPIIIGLVKNKNHQTDHIMLSDFQVKKIAINSPLFHYLATIQTEVDGFAKRSAFNKLSNHQLQNPLLQIPGVGKITAQILFDNFQTLNNIKLASVNELSQFIKKPLAQKIKTYFAKQTD", "aa_hexdigest": "9575d650b881c251d561b19d1cb43ddb", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47448", "query_cov": 1.0, "subject_cov": 0.8140703517587939, "identity": 0.996, "score": 957.0, "evalue": 0.0, "valid": true, "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "uniref50_id": "UniRef50_P47448", "cog_id": "COG0322", "cog_category": "L", "kegg_orthology_id": "K03703", "go_ids": [ "GO:0003677", "GO:0005737", "GO:0006289", "GO:0006974", "GO:0009380", "GO:0009381", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P47448", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47448" ], "product": "UvrABC system protein C" }, "genes": [ "uvrC" ], "id": "JPCNHPFPBM_481", "locus": "GCA000292505_00446" }, { "type": "cds", "sequence": "contig_1", "start": 247405, "stop": 247749, "strand": "+", "frame": 1, "gene": "yfcE", "product": "putative metallophosphoesterase MG207", "db_xrefs": [ "COG:COG0622", "COG:R", "EC:3.1.4.-", "GO:0016787", "GO:0046872", "KEGG:K07095", "SO:0001217", "UniRef:UniRef50_P47449", "UniRef:UniRef90_P47449" ], "nt": "GTGGCAGGTAACAACGATTCAATTGGCAATGAAATTGAAATTTTCCAGTTAGGGCAAATTAACTTTGTGTTAATGCATGGTCACCAAGCACCAAGAGATAACCTGAAAAAGTGGTACCAATTATTAGTTTTAAAAGCACAACAATACCCTTGTGATGTTTTAATCTTTGGTCATAGTCACATTGAATATACCAACAAAATTAATAAGATCCAGTTAATCAACCCTGGTTCTCTACAACTACCAAGAAACCAAACCAACACCCCTTCATACTGTACCTTTATTGTCAATAAAGACGAGCTAACTGATCTAACTATCCACTATTACCAAGCTTCAAAAGTTAGTTAA", "aa": "MAGNNDSIGNEIEIFQLGQINFVLMHGHQAPRDNLKKWYQLLVLKAQQYPCDVLIFGHSHIEYTNKINKIQLINPGSLQLPRNQTNTPSYCTFIVNKDELTDLTIHYYQASKVS", "aa_hexdigest": "ed7f234b411cb1db7364d7bed4f50ba6", "start_type": "GTG", "rbs_motif": null, "genes": [ "yfcE" ], "truncated": "5-prime", "pseudogene": { "start": 247254, "stop": 247749, "observations": { "insertion": [ 247365 ], "stop": [ 247402 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47449", "query_cov": 1.0, "subject_cov": 0.6993865030674846, "identity": 0.982, "score": 235.0, "evalue": 5.13e-78, "gene_start": 1, "gene_end": 114, "reference_start": 50, "reference_end": 163, "reference_sequence": "MIKVLVIADTHGQNQRWIELKNYHNPDVIIHAGDHMTTKQFMDQNATFWVAGNNDSIGNEIEIFQLGQINFVLMHGHQAPRDNLKKWYQLLVLKAQQYPCDVLIFGHSHIEYTNKINMIQLINPGSLQLPRNQTNTPSYCTFIVNKDELTDLTIHYYQASKVS" }, "paralog": false, "identity": 0.975609756097561, "score": 323.0, "evalue": 2.86e-115, "description": "Internal stop codon at 247402. Frameshift due to insertion around 247365.", "psc": { "uniref90_id": "UniRef90_P47449", "gene": "YfcE", "product": "Probable metallophosphoesterase MG207", "ec_ids": [ "3.1.4.-" ], "uniref50_id": "UniRef50_P47449", "cog_id": "COG0622", "cog_category": "R", "kegg_orthology_id": "K07095", "go_ids": [ "GO:0016787", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47449", "EC:3.1.4.-", "UniRef:UniRef50_P47449", "COG:COG0622", "COG:R", "KEGG:K07095", "GO:0016787", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47449", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47449" ], "product": "Probable metallophosphoesterase MG207" } }, "id": "JPCNHPFPBM_482", "locus": "GCA000292505_00447" }, { "type": "cds", "sequence": "contig_1", "start": 247742, "stop": 248332, "strand": "-", "frame": 3, "gene": "tsaB", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB", "db_xrefs": [ "COG:COG1214", "COG:J", "EC:2.3.1.234", "GO:0002949", "RefSeq:WP_009885745.1", "SO:0001217", "UniParc:UPI0000139473", "UniRef:UniRef100_P47450", "UniRef:UniRef50_P47450", "UniRef:UniRef90_P47450" ], "nt": "ATGTTCTTTTTAAGTAAGTATAAGCTGTTTTTAGACTGTGCTTATAAAACCCTTAACATCATTATTTTAGAGATGAAAACCAATGCAGTGGTTGATGAGTTGTCCATTGGTGTTGAACAGAACTTAACTGAACTTGCTGTTTATTATCTGGAGACAATGTTAACAAAAAACAAGTTGAAAAAAAGCAGTATTAAACAGTTTTATGTCACAATTGGCCCTGGTAGTTTCACCGGTCAGAGGATAGCAACTATCATTGCTAAAAGCTGGTGTTTATTGTATCCCAGTTGTGAACTTTATGCTTTAAATTCACTCCGCTTTCAAATCCCATATGAACATGGGATTAGCAAGATTAGTTGTGGTAATGATCAGAACTATTGTGGACTATACAGCCAAACTACTAGTGAAATAAAGCTAATTAGTAAAGCTGATTTTGTGAAGTTATGTAAGGCAAACAATGAACTTCCTATGTATGAAAACTTTGAAAACATTGAGAGTTATAGCAAGCTGTTATTAAGCAACATCGATCACTTTGAAAGAATCGAAGATCCTCTAACCTTACAACCTATCTATCTAAAAGATCCTGTTAACTAA", "aa": "MFFLSKYKLFLDCAYKTLNIIILEMKTNAVVDELSIGVEQNLTELAVYYLETMLTKNKLKKSSIKQFYVTIGPGSFTGQRIATIIAKSWCLLYPSCELYALNSLRFQIPYEHGISKISCGNDQNYCGLYSQTTSEIKLISKADFVKLCKANNELPMYENFENIESYSKLLLSNIDHFERIEDPLTLQPIYLKDPVN", "aa_hexdigest": "99d7d0675e50dd4756b8ad803e1c0ca3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139473", "ncbi_nrp_id": "WP_009885745.1", "uniref100_id": "UniRef100_P47450", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139473", "RefSeq:WP_009885745.1", "UniRef:UniRef100_P47450" ] }, "ips": { "uniref100_id": "UniRef100_P47450", "uniref90_id": "UniRef90_P47450" }, "psc": { "uniref90_id": "UniRef90_P47450", "gene": "tsaB", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_P47450", "cog_id": "COG1214", "cog_category": "J", "go_ids": [ "GO:0002949" ] }, "pscc": { "uniref50_id": "UniRef50_P47450", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47450" ], "product": "Uncharacterized protein MG208" }, "genes": [ "tsaB" ], "id": "JPCNHPFPBM_483", "locus": "GCA000292505_00448" }, { "type": "cds", "sequence": "contig_1", "start": 248316, "stop": 249242, "strand": "-", "frame": 2, "gene": "rluA", "product": "putative RNA pseudouridine synthase MG209", "db_xrefs": [ "COG:COG0564", "COG:J", "EC:5.4.99.-", "GO:0000455", "GO:0003723", "GO:0009982", "GO:0120159", "KEGG:K06180", "RefSeq:WP_009885746.1", "SO:0001217", "UniParc:UPI0000139478", "UniRef:UniRef100_P47451", "UniRef:UniRef50_P47451", "UniRef:UniRef90_P47451" ], "nt": "ATGAAACAGTGTTTTGTTGTTACAACTACCAAACGCTTAGATAGTCTTTTAGCTAGCTTACTGAACCTTTCAAGAGTAAAGGTAGTGAAGCTGATCATGAATGGACAGATTAAAGTTAATGAAAAACTAACTTTTAAAAACAGTTTAATAGTTGCAAAAGATGATGTAATTAAAGTTGAGATTCATGATGAGACAACTAGTGATTTCATTACTAGTGTTGAACCTTATAACTTAAAGCTTGAGGTTCTTTTTGAAGACAAGGATTTGATGGTTATTAACAAACCATCAGGTTTGTTAACCCATCCCACCACTTTCAATGAAAAAGCCAGCTTGTTAGCTGCTTGTATCTTTCACAACAACAAAAACCCTGTTTACTTAGTGCACAGATTGGACCGTGATACTAGTGGGGCAATTGTTGTCTGTAAAAACCAAGCAAGCTTATTAAATTTGCAAAATCAACTGCAAAATCGCACCTTAAAACGTTATTATGTAGCACTAGTCCACTTCCCTTTTAATGCCTTAACTGGTTCAATTAATGCACCTTTAGCAAGGGTTAATAACAACAAGGTAATGTTTAAAATAGCCCAAACTGCTAAAGCAAAGCAAGCAATAACTAAGTTTAAAGTGATTAATCAGAATGAAAAAGCAGCACTAATTAGCTTGGAATTGTTAACAGGTAGAACCCACCAAATTAGAGTGCATCTGAAATTTATCCAACATCCAGTTTATAATGATCCACTGTATGGAATTAAAAGTGAAAAGAAAGATAGCTATGGTCAGTTTCTCCATGCAAACAGGATCTGTTTTATCCATCCCACTTTAAACAAACCTATGGACTTTCACGCCCCACTTGAACCTAAGTTTTCAACGAAACTTAAGAGTTTAAACTTATCTTTAACCGATCCACTCCATGTTCTTTTTAAGTAA", "aa": "MKQCFVVTTTKRLDSLLASLLNLSRVKVVKLIMNGQIKVNEKLTFKNSLIVAKDDVIKVEIHDETTSDFITSVEPYNLKLEVLFEDKDLMVINKPSGLLTHPTTFNEKASLLAACIFHNNKNPVYLVHRLDRDTSGAIVVCKNQASLLNLQNQLQNRTLKRYYVALVHFPFNALTGSINAPLARVNNNKVMFKIAQTAKAKQAITKFKVINQNEKAALISLELLTGRTHQIRVHLKFIQHPVYNDPLYGIKSEKKDSYGQFLHANRICFIHPTLNKPMDFHAPLEPKFSTKLKSLNLSLTDPLHVLFK", "aa_hexdigest": "07f74059485ad672f0972c9e1a18256b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139478", "ncbi_nrp_id": "WP_009885746.1", "uniref100_id": "UniRef100_P47451", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139478", "RefSeq:WP_009885746.1", "UniRef:UniRef100_P47451" ] }, "ips": { "uniref100_id": "UniRef100_P47451", "uniref90_id": "UniRef90_P47451" }, "psc": { "uniref90_id": "UniRef90_P47451", "gene": "RluA", "product": "Uncharacterized RNA pseudouridine synthase MG209", "ec_ids": [ "5.4.99.-" ], "uniref50_id": "UniRef50_P47451", "cog_id": "COG0564", "cog_category": "J", "kegg_orthology_id": "K06180", "go_ids": [ "GO:0009982", "GO:0003723", "GO:0120159", "GO:0000455" ] }, "pscc": { "uniref50_id": "UniRef50_P47451", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47451" ], "product": "Uncharacterized RNA pseudouridine synthase MG209" }, "genes": [ "rluA" ], "id": "JPCNHPFPBM_484", "locus": "GCA000292505_00449" }, { "type": "cds", "sequence": "contig_1", "start": 249449, "stop": 249787, "strand": "-", "frame": 3, "gene": "lspA", "product": "signal peptidase II", "db_xrefs": [ "COG:COG0597", "COG:MU", "EC:3.4.23.36", "GO:0004190", "GO:0005886", "GO:0006508", "GO:0009306", "KEGG:K03101", "SO:0001217", "UniRef:UniRef50_P75484", "UniRef:UniRef90_Q49401" ], "nt": "ATGAAATTAAGAAAAACCAAGTTTTTTTCACAACTTAAACACCAGGTTTTAACTGCAAACCAAAAACCATTTTTATTCTATAAACTGACAATGATTGGGTTTGTTGGCTTTATTATCTTACTGCAAGTTTTCATATTAAGAAATGCGTTAAATGGTGAGATGGATAACACCATGGTAGCAAATAGTGGTTTTATTAATATCTATGTGATTAGAAACAAAGGGGTAGGGTTTAGCTTATTACAAAACCAAACTGGCTTAGTTTACTTTCTCCAGGGATTATTATCAGTAATTGCGTTAGTTTTTCTTGTTTTTATGGTGAAATATAGTTACATCTTTTGA", "aa": "MKLRKTKFFSQLKHQVLTANQKPFLFYKLTMIGFVGFIILLQVFILRNALNGEMDNTMVANSGFINIYVIRNKGVGFSLLQNQTGLVYFLQGLLSVIALVFLVFMVKYSYIF", "aa_hexdigest": "d57fe133b1b7b575ef9c508d83493b3e", "start_type": "ATG", "rbs_motif": null, "genes": [ "lspA" ], "truncated": "5-prime", "pseudogene": { "start": 249242, "stop": 249787, "observations": { "mutation": [ 249451 ], "stop": [ 249451 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49401", "query_cov": 1.0, "subject_cov": 0.6187845303867403, "identity": 1.0, "score": 207.0, "evalue": 1.61e-66, "gene_start": 1, "gene_end": 112, "reference_start": 1, "reference_end": 112, "reference_sequence": "MKLRKTKFFSQLKHQVLTANQKPFLFYKLTMIGFVGFIILLQVFILRNALNGEMDNTMVANSGFINIYVIRNKGVGFSLLQNQTGLVYFLQGLLSVIALVFLVFMVKYSYIFWITTLAFGSLGNFFDRLTSANDSVLDYFIFQNGSSVFNFADCCITFGFIGLFFCFLIQMFKEFKHSKNQ" }, "paralog": false, "identity": 0.994475138121547, "score": 358.0, "evalue": 1.45e-128, "description": "Internal stop codon at 249451. Nonsense mutation around 249451.", "psc": { "uniref90_id": "UniRef90_Q49401", "gene": "lspA", "product": "signal peptidase II", "ec_ids": [ "3.4.23.36" ], "uniref50_id": "UniRef50_P75484", "cog_id": "COG0597", "cog_category": "MU", "kegg_orthology_id": "K03101", "go_ids": [ "GO:0004190", "GO:0005886", "GO:0006508", "GO:0009306" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49401", "EC:3.4.23.36", "UniRef:UniRef50_P75484", "COG:COG0597", "COG:MU", "KEGG:K03101", "GO:0004190", "GO:0005886", "GO:0006508", "GO:0009306" ] }, "pscc": { "uniref50_id": "UniRef50_P75484", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75484" ], "product": "Lipoprotein signal peptidase" } }, "id": "JPCNHPFPBM_485", "locus": "GCA000292505_00450" }, { "type": "cds", "sequence": "contig_1", "start": 250390, "stop": 250635, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCTTAGTTGGTTTACCCTTTGAAAAAGGTGCTTATCATAAGCAGTTATATGACCATTCACGTCAGGAGATCTTAGATCTTACTGAAGTTATTAAAAAGCTTTATCTAAAAACAGAGACCAACAACGATAAGCTTGAGTTTGCTGCAACTACTAGTAAAACAACAGCGCAGCTAACTAAAACTATGCCCTTAAACAGTAGTGATCTTGATAAGGATCTAATGGAGTTTAGTGGTGAGAAGTGA", "aa": "MILVGLPFEKGAYHKQLYDHSRQEILDLTEVIKKLYLKTETNNDKLEFAATTSKTTAQLTKTMPLNSSDLDKDLMEFSGEK", "aa_hexdigest": "9b80defb3d37c571d723cb3422c4fe48", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9266.492899999997, "isoelectric_point": 5.603665351867675 }, "id": "JPCNHPFPBM_486", "locus": "GCA000292505_00451" }, { "type": "cds", "sequence": "contig_1", "start": 250647, "stop": 250835, "strand": "+", "frame": 3, "gene": "rpsU", "product": "30S ribosomal protein S21", "db_xrefs": [ "COG:COG0828", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_009885749.1", "SO:0001217", "UniParc:UPI0000134E7C", "UniRef:UniRef100_P57085", "UniRef:UniRef50_Q8EUK4", "UniRef:UniRef90_P57079" ], "nt": "GTGCCTAAGATTGAAGTTAAAAATGATGATTTAGAGCTAGCTTTAAAAAAGTTTAAAAGGATATCACTGGAAGTACGCAGGTTAGCACAACGCCATGAATACCACTTGCGCAAAGGGATGCGGTTAAGAGAAAAACAAAAGATAGCACAGAAAAAGCGCAGGAAGTTTCGCAGTTTAGCTAGCCATTAA", "aa": "MPKIEVKNDDLELALKKFKRISLEVRRLAQRHEYHLRKGMRLREKQKIAQKKRRKFRSLASH", "aa_hexdigest": "9ff4ed1fff707dabf05dea47bbb375a5", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134E7C", "ncbi_nrp_id": "WP_009885749.1", "uniref100_id": "UniRef100_P57085", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134E7C", "RefSeq:WP_009885749.1", "UniRef:UniRef100_P57085" ] }, "ips": { "uniref100_id": "UniRef100_P57085", "uniref90_id": "UniRef90_P57079" }, "psc": { "uniref90_id": "UniRef90_P57079", "gene": "rpsU", "product": "30S ribosomal protein S21", "uniref50_id": "UniRef50_Q8EUK4", "cog_id": "COG0828", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q8EUK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8EUK4" ], "product": "Small ribosomal subunit protein bS21" }, "genes": [ "rpsU" ], "id": "JPCNHPFPBM_487", "locus": "GCA000292505_00452" }, { "type": "cds", "sequence": "contig_1", "start": 250837, "stop": 251235, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MG211", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47453", "UniRef:UniRef90_P47453" ], "nt": "ATGGATAATAAAAACCCCCAGAAACTTATTACTAGTGAATTGTTGGCAAACCACCGCTTTAATTTTGCTAAAGATGATAAAGGTGGGTATGATGCTAATGAAGTTGATGCATTCTTAGATCAACTAACCAAGACTTTAATCCACTATGAGGAGATGAAAAACAACGAACAAGAATTGAAAAATGCTTATGACAAGTTGTTTTCAGATCGTGATCAGATTTTAAGTCGTTGTGCTAAATTAGAAGCTGATTTAAACACCTTTTATGAAAATGGTTATGCAAACAAGGTGTTAATTAACCGGGTTCAGGAGTTGGAGGATAAACTTGAAAAACTACCTGATCGTTACACTGAAAAACTAGAAAGGATTGAAAAACTGTTAAAAAAGGTCATTAAACACTGA", "aa": "MDNKNPQKLITSELLANHRFNFAKDDKGGYDANEVDAFLDQLTKTLIHYEEMKNNEQELKNAYDKLFSDRDQILSRCAKLEADLNTFYENGYANKVLINRVQELEDKLEKLPDRYTEKLERIEKLLKKVIKH", "aa_hexdigest": "e87fa732e2b19cb9f0e9d399a42f753a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47453", "query_cov": 1.0, "subject_cov": 0.8979591836734694, "identity": 1.0, "score": 258.0, "evalue": 6.36e-87, "valid": true, "product": "Uncharacterized protein MG211", "uniref50_id": "UniRef50_P47453" }, "pscc": { "uniref50_id": "UniRef50_P47453", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47453" ], "product": "Uncharacterized protein MG211" }, "genes": [], "id": "JPCNHPFPBM_488", "locus": "GCA000292505_00453" }, { "type": "cds", "sequence": "contig_1", "start": 251283, "stop": 251399, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGTTGGGATAGGGATTGATGTTGTGCAATTAAAGCGCTTCTTAACTTTAGTTGAAACTAGTGATTGTTTTGCTAAACGATTGTTAACTAGCAATGAACTAAACAGTTATTGA", "aa": "MVVGIGIDVVQLKRFLTLVETSDCFAKRLLTSNELNSY", "aa_hexdigest": "a13dc5c3893cda2b5abaf11f801c8775", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4273.9681, "isoelectric_point": 5.968969535827637 }, "id": "JPCNHPFPBM_489", "locus": "GCA000292505_00454" }, { "type": "cds", "sequence": "contig_1", "start": 251620, "stop": 251814, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAACTATTTAAAACAAGTTTTAGATTCATAATAAGGTTTTTACAAATCCTGAGTTTACCAGTTGTTTTTCCTTACTTTTTATTAAGCTTTTTAGCTTGTTTAATTACTAGTAAAAACTATGAATCACTCCCTTATAACTATCCCCCTGAAATCCGGTTCAAAAAGGTGTATAGATTGGTATCAATGTGA", "aa": "MDKLFKTSFRFIIRFLQILSLPVVFPYFLLSFLACLITSKNYESLPYNYPPEIRFKKVYRLVSM", "aa_hexdigest": "0580a8defe65587357de48bb24e59c8b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7747.274199999999, "isoelectric_point": 9.841888618469238 }, "id": "JPCNHPFPBM_490", "locus": "GCA000292505_00455" }, { "type": "cds", "sequence": "contig_1", "start": 251839, "stop": 252426, "strand": "+", "frame": 1, "gene": "plsC", "product": "putative 1-acyl-sn-glycerol-3-phosphate acyltransferase", "db_xrefs": [ "COG:COG0204", "COG:I", "EC:2.3.1.51", "GO:0003841", "GO:0006654", "GO:0016020", "GO:0016024", "SO:0001217", "UniRef:UniRef50_Q49402", "UniRef:UniRef90_Q49402" ], "nt": "GTGACAGTAAATGACAAGATTATCCCTAAAAAACCAGTTTTAGTGGTAGCTAACCACAAATCTAACCTTGATCCTTTAGTATTAATTAAGGCCTTTGGCAGGTTGAAAAATAGTCCACCATTAACCTTTGTTGCTAAGATTGAACTGAAAGATACAGTCCTTTTTAAACTGATGAAATTAATTGATTGTGTTTTTATTGATCGAAAAAACATCAGACAAATTGCCAATGCATTGGAAACCCAACAACAACTAATTCGCCAGGGCACTGCTATTGCTGTTTTTGCTGAAGGGACTAGGATTTTAAGTAATGACATTGGGGAATTTAAACCAGGAGCACTAAAGGTTGCTTACAATGCTTTTGTACCTATCTTACCAGTTAGTATTGTGGGTAGCTTAGGAAAGATGGAATCAAACAAAAGGCTAAAAGAACATGGTGTTAAGAAAAGTTCAAACTATGAGGTTAAAGTAATCTTTAACAAGCTAATTAACCCAATTAGTTTTAACCAGATTGATTCTAATAACCTTGCTAATAACATTAGAAGCATTATTAGTGATGCATACACTAGTGAAAAACCAAGCAATGATTAG", "aa": "MTVNDKIIPKKPVLVVANHKSNLDPLVLIKAFGRLKNSPPLTFVAKIELKDTVLFKLMKLIDCVFIDRKNIRQIANALETQQQLIRQGTAIAVFAEGTRILSNDIGEFKPGALKVAYNAFVPILPVSIVGSLGKMESNKRLKEHGVKKSSNYEVKVIFNKLINPISFNQIDSNNLANNIRSIISDAYTSEKPSND", "aa_hexdigest": "622239409f0f6bb20e12654bc302dbb7", "start_type": "GTG", "rbs_motif": null, "genes": [ "plsC" ], "truncated": "5-prime", "pseudogene": { "start": 251620, "stop": 252426, "observations": { "mutation": [ 251812 ], "stop": [ 251812 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49402", "query_cov": 1.0, "subject_cov": 0.7276119402985075, "identity": 0.995, "score": 369.0, "evalue": 1.01e-127, "gene_start": 1, "gene_end": 195, "reference_start": 74, "reference_end": 268, "reference_sequence": "MDKLFKTSFRFIIRFLQILSLPVVFPYFLLSFLACLITSKNYESLPYNYPPEIRFKKVYRLVSMWLYIKGIKVVTVNDKIIPKKPVLVVANHKSNLDPLVLIKAFGRLKNSPPLTFVAKIELKDTVLFKLMKLIDCVFIDRKNIRQIANALETQQQLIRQGTAIAVFAEGTRILSNDIGEFKPGALKVAYNAFVPILPVSIVGSLGKMESNKRLKEHGVKKSSNYEVKVIFNKLINPISFNQIDSNNLANNIRSIISDAYTSEKPSND" }, "paralog": false, "identity": 0.996268656716418, "score": 518.0, "evalue": 2.67e-189, "description": "Internal stop codon at 251812. Nonsense mutation around 251812.", "psc": { "uniref90_id": "UniRef90_Q49402", "gene": "plsC", "product": "Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase", "ec_ids": [ "2.3.1.51" ], "uniref50_id": "UniRef50_Q49402", "cog_id": "COG0204", "cog_category": "I", "go_ids": [ "GO:0016020", "GO:0003841", "GO:0016024", "GO:0006654" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49402", "EC:2.3.1.51", "UniRef:UniRef50_Q49402", "COG:COG0204", "COG:I", "GO:0016020", "GO:0003841", "GO:0016024", "GO:0006654" ] }, "pscc": { "uniref50_id": "UniRef50_Q49402", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49402" ], "product": "Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase" } }, "id": "JPCNHPFPBM_491", "locus": "GCA000292505_00456" }, { "type": "cds", "sequence": "contig_1", "start": 252593, "stop": 252781, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAACCGAATTAACCCCAAATTCAAAACTCACAGTTTTTAACAACTACCAGCAACTTTTAGTTGATAATAATGACCTAATTATCTTTGCAACCCCCACTTTAGTTCAGCTTTTTGATAATGAGATTGACCAGTTAATAGTGATCAATCCCACTAGTAAATCTAAAGATCAGTTTAACTGTAATTGA", "aa": "MQTELTPNSKLTVFNNYQQLLVDNNDLIIFATPTLVQLFDNEIDQLIVINPTSKSKDQFNCN", "aa_hexdigest": "ada697e9a5f1dabb980c0eb2747ea4bf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7115.954700000001, "isoelectric_point": 4.089929389953612 }, "id": "JPCNHPFPBM_492", "locus": "GCA000292505_00457" }, { "type": "cds", "sequence": "contig_1", "start": 252950, "stop": 253834, "strand": "+", "frame": 2, "gene": null, "product": "segregation/condensation protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF5CFDF" ], "nt": "GTGGATATTGGCTTGATTTCCTTACAATATCTCAATATCATTGCAAATTACACCAATAAAAAAGCCATTGAAAAGATCACTGATTATCTGGTGATTACAAGCAAAATTCTCGCTAAAAAAGCAGATAATCTGCTCAATGATCATCAGGAAGAGAGTGTGTTGGAATATGATTTAGCTACTAATAACTTTCGTGATAAGATGATCGCTAACTTAGTGGAACACAAACGCTATTGTGATTCACTGGGTGAATTTGAAAAACTCAGGGTTAACCGCTTAGCTTACTTTTCCAAAGCTAATGAGATGGAGCAATTTATCAAAACAGCTAATGATCAACTTGTTACAGTTGAAGACCAACTCCCTAACTATATTAGTGTCTTAAAACTCTTTCATGCTATGAACAAGTTACTGGAGATGAGACTCAGCTCTCTTCTAACAAATAAAAACATCACCATTAAAGAGTTATCAGTTGAACAGGTTCAAAAGGAGTTGGTTTTAGCAATCAAACAGTTTAACTACCAAACAGTTTCTTTAAAACGGGTGTTATTAAAACTTAACCATCCTATCTCTTTAATGTATTTTGTTACTGCTTTTGTGGCACTTTTAGTGCTATTAAACAACCAGGTAATAGGTTTGGAACAAAAAGATTATCACAGTGAACTTTACATCTTTTTACTAGATGAAAACCAACTGAAAACCTTCCAAGAATCACCAGATGAAATGGTAAAAAGAATCCAAGCTCAACAACAGCAAAACGAACTGATAATTGCTAAAAACAAGCAACTAAGAGCTATCAAAAACAAACAAAAGCGAGCTGATTATCTAAAAAAGAAGTATGGTGAAAATTACTTAGATAAAACTAACTTAAAAGATGAAAACAACAATTAA", "aa": "MDIGLISLQYLNIIANYTNKKAIEKITDYLVITSKILAKKADNLLNDHQEESVLEYDLATNNFRDKMIANLVEHKRYCDSLGEFEKLRVNRLAYFSKANEMEQFIKTANDQLVTVEDQLPNYISVLKLFHAMNKLLEMRLSSLLTNKNITIKELSVEQVQKELVLAIKQFNYQTVSLKRVLLKLNHPISLMYFVTAFVALLVLLNNQVIGLEQKDYHSELYIFLLDENQLKTFQESPDEMVKRIQAQQQQNELIIAKNKQLRAIKNKQKRADYLKKKYGENYLDKTNLKDENNN", "aa_hexdigest": "15fb8a2ef922a4dc4c61d5687f1bb74c", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000AF5CFDF", "query_cov": 0.9727891156462585, "subject_cov": 0.8614457831325302, "identity": 0.552, "score": 279.0, "evalue": 6.7e-90, "valid": false, "gene": "scpA", "product": "segregation/condensation protein A", "uniref50_id": "UniRef50_UPI000AF5CFDF", "kegg_orthology_id": "K05896" }, "pscc": { "uniref50_id": "UniRef50_UPI000AF5CFDF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF5CFDF" ], "product": "segregation/condensation protein A" }, "genes": [], "id": "JPCNHPFPBM_493", "locus": "GCA000292505_00458" }, { "type": "cds", "sequence": "contig_1", "start": 253818, "stop": 254441, "strand": "+", "frame": 3, "gene": "scpB", "product": "SMC-Scp complex subunit ScpB", "db_xrefs": [ "COG:COG1386", "COG:K", "GO:0005515", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0051301", "GO:0051304", "KEGG:K06024", "RefSeq:WP_009885754.1", "SO:0001217", "UniParc:UPI0000139487", "UniRef:UniRef100_P47456", "UniRef:UniRef50_P75477", "UniRef:UniRef90_P47456" ], "nt": "ATGAAAACAACAATTAATATTGCCACCCCTACCCTAAAAAAACCTAGCAAAGAAGCTAACTTGGTTGCTAGTATCTATGGGTTGTTATTTGTTTGTGGCGCGAAAGGGATCACTTTAAGAGAACTAATTAGGATCTTTAAAAAAGCAGGGATTGAAAAGGTGAAATTAGCACTCTTAGCACTTGAAAGGAAGTTAGCAGATGATGAGCAATCAGGAGTGGAGTTGAAAAAATTTGGTAATAGTTTTTCTTTGGTAACAAAACCAATTATCAAAGACTATCTCCACTTATTATTGGCTCATAAAGTCAAAAATCCCCTTAATTCCAAAGCAATGGAAGTGTTGGCTATCATTGCTTACAACCAACCTTGCACCAGACCCAGAATTAATGAAATTAGGGGAGTTGATTCTTTTCAAATTGTTGATGATCTAATAGCAAAGGAGTTAATTGTGGAGTTAGGGAGAACTGATAAACCAGGTCGACCTTTTATTTATGAAGTGTCAGCTAAGTTCTATGATTTATTTGGCATTGATAGCTTAGATCAACTCCCTAAGATTGAGCATTTTGATCTTGATAAATTTAAGCAAGGTAGCTTTTTTGATTCCAACCGCTATGGTGATGAATAA", "aa": "MKTTINIATPTLKKPSKEANLVASIYGLLFVCGAKGITLRELIRIFKKAGIEKVKLALLALERKLADDEQSGVELKKFGNSFSLVTKPIIKDYLHLLLAHKVKNPLNSKAMEVLAIIAYNQPCTRPRINEIRGVDSFQIVDDLIAKELIVELGRTDKPGRPFIYEVSAKFYDLFGIDSLDQLPKIEHFDLDKFKQGSFFDSNRYGDE", "aa_hexdigest": "1dd1f4609d2ad8e124f79d2eb5f29b0d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139487", "ncbi_nrp_id": "WP_009885754.1", "uniref100_id": "UniRef100_P47456", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139487", "RefSeq:WP_009885754.1", "UniRef:UniRef100_P47456" ] }, "ips": { "uniref100_id": "UniRef100_P47456", "uniref90_id": "UniRef90_P47456" }, "psc": { "uniref90_id": "UniRef90_P47456", "gene": "scpB", "product": "SMC-Scp complex subunit ScpB", "uniref50_id": "UniRef50_P75477", "cog_id": "COG1386", "cog_category": "K", "kegg_orthology_id": "K06024", "go_ids": [ "GO:0005515", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0051301", "GO:0051304" ] }, "pscc": { "uniref50_id": "UniRef50_P75477", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75477" ], "product": "Segregation and condensation protein B" }, "genes": [ "scpB" ], "id": "JPCNHPFPBM_494", "locus": "GCA000292505_00459" }, { "type": "cds", "sequence": "contig_1", "start": 254460, "stop": 254879, "strand": "+", "frame": 3, "gene": "pfkA", "product": "putative ATP-dependent 6-phosphofructokinase", "db_xrefs": [ "COG:COG0205", "COG:G", "EC:2.7.1.11", "GO:0003872", "GO:0005524", "GO:0005737", "GO:0006002", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P75476", "UniRef:UniRef90_P47457" ], "nt": "ATGGATAAAATAGCTATTTTAACTTCGGGTGGTGATGCTAGTGGGATGAATGCCACCATCGCTTATCTAACCAAATATGCAATTGCAAAGCAATTGGAAGTTTTTTATGTAAAAAACGGTTATTATGGCTTGTATCACAACCATTTTATCACCAGTAAGGAACTTGATTTAACTGACTTTTTCTTTATGGGGGGAACAGTAATAGGATCAAGTCGTTTCAAACAGTTTCAAGATCCTAGCTTACGAAAACAAGCAGTTTTAAACCTCAAAAAACGTGGTATTAACAACCTTGTTGTTATTGGTGGGGATGGGAGTTATATGGGTGCTAAAGCACTCAGTGAATTAGGATTAAACTGCTTTTGTTTACCTGGTACGATCGACAATGATGTCAATTCCAGTGAATTTACCATTGGTTTTTGA", "aa": "MDKIAILTSGGDASGMNATIAYLTKYAIAKQLEVFYVKNGYYGLYHNHFITSKELDLTDFFFMGGTVIGSSRFKQFQDPSLRKQAVLNLKKRGINNLVVIGGDGSYMGAKALSELGLNCFCLPGTIDNDVNSSEFTIGF", "aa_hexdigest": "7d4c7a73cc710480e6da8e0a33dd1a92", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2X1PN14", "query_cov": 0.9928057553956835, "subject_cov": 0.8846153846153846, "identity": 0.514, "score": 135.0, "evalue": 4e-38, "valid": false, "product": "6-phosphofructokinase", "uniref50_id": "UniRef50_A0A2X1PN14" }, "genes": [ "pfkA" ], "truncated": "3-prime", "pseudogene": { "start": 254460, "stop": 255182, "observations": { "mutation": [ 254877 ], "stop": [ 254877 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47457", "query_cov": 1.0, "subject_cov": 0.43034055727554177, "identity": 1.0, "score": 279.0, "evalue": 1.27e-92, "gene_start": 1, "gene_end": 139, "reference_start": 1, "reference_end": 139, "reference_sequence": "MDKIAILTSGGDASGMNATIAYLTKYAIAKQLEVFYVKNGYYGLYHNHFITSKELDLTDFFFMGGTVIGSSRFKQFQDPSLRKQAVLNLKKRGINNLVVIGGDGSYMGAKALSELGLNCFCLPGTIDNDVNSSEFTIGFWTALEAIRVNVEAIYHTTKSHNRLAIIEVMGRDCSDLTIFGGLATNASFVVTSKNSLDLNGFEKAVRKVLQFQNYCVVLVSENIYGKNGLPSLEMVKEHFENNAIKCNLVSLGHTQRGFSPNSIELFQISLMAKHTIDLVVNNANSQVIGMKNNQAVNYDFNTAFNLPKADRTKLLNQVNTAII" }, "paralog": false, "identity": 0.9958333333333333, "score": 475.0, "evalue": 3.62e-172, "description": "Internal stop codon at 254877. Nonsense mutation around 254877.", "psc": { "uniref90_id": "UniRef90_P47457", "gene": "pfkA", "product": "Probable ATP-dependent 6-phosphofructokinase", "ec_ids": [ "2.7.1.11" ], "uniref50_id": "UniRef50_P75476", "cog_id": "COG0205", "cog_category": "G", "go_ids": [ "GO:0005737", "GO:0003872", "GO:0005524", "GO:0046872", "GO:0006002" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47457", "EC:2.7.1.11", "UniRef:UniRef50_P75476", "COG:COG0205", "COG:G", "GO:0005737", "GO:0003872", "GO:0005524", "GO:0046872", "GO:0006002" ] }, "pscc": { "uniref50_id": "UniRef50_P75476", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75476" ], "product": "Probable ATP-dependent 6-phosphofructokinase" } }, "id": "JPCNHPFPBM_495", "locus": "GCA000292505_00460" }, { "type": "cds", "sequence": "contig_1", "start": 254964, "stop": 255431, "strand": "+", "frame": 3, "gene": "pfkA", "product": "putative ATP-dependent 6-phosphofructokinase", "db_xrefs": [ "COG:COG0205", "COG:G", "EC:2.7.1.11", "GO:0003872", "GO:0005524", "GO:0005737", "GO:0006002", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P75476", "UniRef:UniRef90_P47457" ], "nt": "ATGGGGCGTGATTGTAGTGATCTGACCATCTTTGGGGGGTTAGCTACTAATGCTAGTTTTGTTGTTACTAGCAAAAATAGCTTGGATCTCAATGGCTTTGAAAAAGCAGTGAGAAAGGTGTTGCAATTCCAGAACTATTGTGTTGTTTTGGTTAGTGAAAACATCTATGGTAAGAACGGTTTACCTAGTTTAGAAATGGTTAAAGAGCACTTTGAAAACAACGCAATTAAGTGTAACCTAGTTTCACTAGGACACACCCAAAGGGGCTTTAGTCCTAATAGTATCGAACTCTTTCAGATTAGTTTAATGGCTAAACACACGATTGATCTGGTTGTAAATAATGCCAACAGTCAAGTAATAGGGATGAAAAACAACCAAGCAGTTAACTATGATTTTAACACTGCTTTTAATTTACCAAAAGCTGATAGAACCAAGTTACTTAACCAAGTTAACACTGCAATTATTTAA", "aa": "MGRDCSDLTIFGGLATNASFVVTSKNSLDLNGFEKAVRKVLQFQNYCVVLVSENIYGKNGLPSLEMVKEHFENNAIKCNLVSLGHTQRGFSPNSIELFQISLMAKHTIDLVVNNANSQVIGMKNNQAVNYDFNTAFNLPKADRTKLLNQVNTAII", "aa_hexdigest": "36160e26e6bbf3b864ec88eb0d2db9a1", "start_type": "ATG", "rbs_motif": null, "genes": [ "pfkA" ], "truncated": "5-prime", "pseudogene": { "start": 254664, "stop": 255431, "observations": { "mutation": [ 254877 ], "stop": [ 254877 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47457", "query_cov": 1.0, "subject_cov": 0.47987616099071206, "identity": 1.0, "score": 306.0, "evalue": 5.71e-103, "gene_start": 1, "gene_end": 155, "reference_start": 169, "reference_end": 323, "reference_sequence": "MDKIAILTSGGDASGMNATIAYLTKYAIAKQLEVFYVKNGYYGLYHNHFITSKELDLTDFFFMGGTVIGSSRFKQFQDPSLRKQAVLNLKKRGINNLVVIGGDGSYMGAKALSELGLNCFCLPGTIDNDVNSSEFTIGFWTALEAIRVNVEAIYHTTKSHNRLAIIEVMGRDCSDLTIFGGLATNASFVVTSKNSLDLNGFEKAVRKVLQFQNYCVVLVSENIYGKNGLPSLEMVKEHFENNAIKCNLVSLGHTQRGFSPNSIELFQISLMAKHTIDLVVNNANSQVIGMKNNQAVNYDFNTAFNLPKADRTKLLNQVNTAII" }, "paralog": false, "identity": 0.996078431372549, "score": 501.0, "evalue": 2.13e-182, "description": "Internal stop codon at 254877. Nonsense mutation around 254877.", "psc": { "uniref90_id": "UniRef90_P47457", "gene": "pfkA", "product": "Probable ATP-dependent 6-phosphofructokinase", "ec_ids": [ "2.7.1.11" ], "uniref50_id": "UniRef50_P75476", "cog_id": "COG0205", "cog_category": "G", "go_ids": [ "GO:0005737", "GO:0003872", "GO:0005524", "GO:0046872", "GO:0006002" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47457", "EC:2.7.1.11", "UniRef:UniRef50_P75476", "COG:COG0205", "COG:G", "GO:0005737", "GO:0003872", "GO:0005524", "GO:0046872", "GO:0006002" ] }, "pscc": { "uniref50_id": "UniRef50_P75476", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75476" ], "product": "Probable ATP-dependent 6-phosphofructokinase" } }, "id": "JPCNHPFPBM_496", "locus": "GCA000292505_00461" }, { "type": "cds", "sequence": "contig_1", "start": 255434, "stop": 256297, "strand": "+", "frame": 2, "gene": "pyk", "product": "pyruvate kinase", "db_xrefs": [ "COG:COG0469", "COG:G", "EC:2.7.1.40", "GO:0000287", "GO:0004743", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006096", "GO:0016301", "GO:0030955", "KEGG:K00873", "SO:0001217", "UniRef:UniRef50_P78031", "UniRef:UniRef90_P47458" ], "nt": "ATGATTGACCATTTAAAAAGAACAAAGATAATCGCTACCTGTGGCCCAGCTTTAACAAAAAGCTTGGTTAGCTTAAAGATGCTTGATGATAATGAGTATGCAGCTATTAAAAAGGTTGCTTATGCCAACATTGAAGCAATTATTAAAAGTGGGGTTAGTGTGATTAGGCTTAACTTCTCTCATGGTACCCATGAAGAACAACAAGTGAGGATCAAGATAGTAAGGGATGTAGCGAAAGCAATGAACATCCCTGTTTCTATTATGTTAGATACAAATGGTCCTGAGATCAGGATAGTAGAAACTAAAAAAGAGGGTTTGAAAATCACCAAAGATAGTGAAGTGATTATCAACACCATGAGTAAAATGATCGCTAGTGACAACCAGTTTGCTGTCAGTGATGCTAGTGGCAAATACAACATGGTTAATGATGTGAATATAGGTCAGAAAATCCTTGTTGATGATGGTAAGTTAACCCTGGTTGTCACAAGGGTTGACAAACAACATAACCAGGTTATCTGTGTTGCAAAAAACGACCACACAGTTTTCACTAAAAAAAGACTTAACCTACCCAACGCACAGTACTCTATCCCTTTTCTCAGTGAAAAGGATCTGAAGGATATTGACTTTGGTTTAAGCCAAGGTATTGACTATATTGCTGCCTCTTTTGTTAATACTGTTGCAGATATTAAACAACTGAGAGATTATCTGAAATTAAAGAATGCTAGTGGGGTGAAGATCATCGCTAAGATTGAATCTAATCATGCTTTAAATAACATTGATAAGATCATTAAAGCTAGCGATGGGATTATGGTTGCTAGGGGTGATTTGGGCCTTGAAATCCCTTATTACCAAGTCCCTTACTGA", "aa": "MIDHLKRTKIIATCGPALTKSLVSLKMLDDNEYAAIKKVAYANIEAIIKSGVSVIRLNFSHGTHEEQQVRIKIVRDVAKAMNIPVSIMLDTNGPEIRIVETKKEGLKITKDSEVIINTMSKMIASDNQFAVSDASGKYNMVNDVNIGQKILVDDGKLTLVVTRVDKQHNQVICVAKNDHTVFTKKRLNLPNAQYSIPFLSEKDLKDIDFGLSQGIDYIAASFVNTVADIKQLRDYLKLKNASGVKIIAKIESNHALNNIDKIIKASDGIMVARGDLGLEIPYYQVPY", "aa_hexdigest": "792210993a97586d312b7cc544bc540e", "start_type": "ATG", "rbs_motif": null, "genes": [ "pyk" ], "truncated": "3-prime", "pseudogene": { "start": 255434, "stop": 256600, "observations": { "mutation": [ 256295 ], "stop": [ 256295 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47458", "query_cov": 1.0, "subject_cov": 0.5649606299212598, "identity": 1.0, "score": 556.0, "evalue": 1.49e-196, "gene_start": 1, "gene_end": 287, "reference_start": 1, "reference_end": 287, "reference_sequence": "MIDHLKRTKIIATCGPALTKSLVSLKMLDDNEYAAIKKVAYANIEAIIKSGVSVIRLNFSHGTHEEQQVRIKIVRDVAKAMNIPVSIMLDTNGPEIRIVETKKEGLKITKDSEVIINTMSKMIASDNQFAVSDASGKYNMVNDVNIGQKILVDDGKLTLVVTRVDKQHNQVICVAKNDHTVFTKKRLNLPNAQYSIPFLSEKDLKDIDFGLSQGIDYIAASFVNTVADIKQLRDYLKLKNASGVKIIAKIESNHALNNIDKIIKASDGIMVARGDLGLEIPYYQVPYWQRYMIKACRFFNKRSITATQMLDSLEKNIQPTRAEVTDVYFAVDRGNDATMLSGETASGLYPLNAVAVMQKIDKQSETFFDYQYNVNYYLKNSTANKSRFWHNVVLPLTKKTVPKRKLVNSAFKYDFIVYPTNNINRIYALSNARLAAAVIILTNNKRVYTGHGVDYGIFCYLIDKNPNQLTKAELIELAWKAINHYQAYGDLEKLKQCLAVYNETIINL" }, "paralog": false, "identity": 0.9974226804123711, "score": 752.0, "evalue": 2.72e-276, "description": "Internal stop codon at 256295. Nonsense mutation around 256295.", "psc": { "uniref90_id": "UniRef90_P47458", "gene": "pyk", "product": "pyruvate kinase", "ec_ids": [ "2.7.1.40" ], "uniref50_id": "UniRef50_P78031", "cog_id": "COG0469", "cog_category": "G", "kegg_orthology_id": "K00873", "go_ids": [ "GO:0000287", "GO:0004743", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006096", "GO:0016301", "GO:0030955" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47458", "EC:2.7.1.40", "UniRef:UniRef50_P78031", "COG:COG0469", "COG:G", "KEGG:K00873", "GO:0000287", "GO:0004743", "GO:0005524", "GO:0005737", "GO:0005829", "GO:0006096", "GO:0016301", "GO:0030955" ] }, "pscc": { "uniref50_id": "UniRef50_P78031", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78031" ], "product": "Pyruvate kinase" } }, "id": "JPCNHPFPBM_497", "locus": "GCA000292505_00462" }, { "type": "cds", "sequence": "contig_1", "start": 256307, "stop": 256600, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00224.27" ], "nt": "ATGATTAAAGCTTGTCGCTTTTTTAACAAGCGTTCTATTACTGCAACCCAAATGCTTGATTCACTAGAAAAAAACATCCAACCAACCCGAGCTGAAGTGACTGATGTTTACTTTGCAGTTGATCGGGGTAATGATGCAACTATGTTAAGTGGGGAAACTGCTAGTGGGCTTTACCCTTTAAATGCAGTAGCGGTGATGCAAAAGATTGATAAACAATCAGAAACCTTCTTTGATTACCAGTATAACGTTAACTATTATTTGAAAAACTCCACGGCAAATAAAAGTAGGTTTTGA", "aa": "MIKACRFFNKRSITATQMLDSLEKNIQPTRAEVTDVYFAVDRGNDATMLSGETASGLYPLNAVAVMQKIDKQSETFFDYQYNVNYYLKNSTANKSRF", "aa_hexdigest": "d15edec308c290aec65533dd551eabf1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00224.27", "name": "PK", "length": 63, "aa_cov": 0.6494845360824743, "hmm_cov": 1.0, "evalue": 2.2263205575480146e-23, "score": 77.8996353149414, "start": 1, "stop": 63 } ], "seq_stats": { "molecular_weight": 11096.425699999994, "isoelectric_point": 8.732000923156736 }, "id": "JPCNHPFPBM_498", "locus": "GCA000292505_00463" }, { "type": "tRNA", "sequence": "contig_1", "start": 256997, "stop": 257073, "strand": "+", "gene": "trnC", "product": "tRNA-Cys(gca)", "amino_acid": "Cys", "anti_codon": "gca", "score": 60.0, "nt": "GGTGCCTTAGCCAAGTGGACTCAAGGCCTGGAGCTGCAACCTCCATATCGTCAGTTCGAATCTGACAGGCACCTCCA", "db_xrefs": [ "SO:0000258" ], "anti_codon_pos": [ 257032, 257034 ], "id": "JPCNHPFPBM_1", "locus": "GCA000292505_00464" }, { "type": "tRNA", "sequence": "contig_1", "start": 257108, "stop": 257184, "strand": "+", "gene": "trnP", "product": "tRNA-Pro(tgg)", "amino_acid": "Pro", "anti_codon": "tgg", "score": 83.1, "nt": "CGGGAAGTAGCTTAGTTTGGTAGAGCACTTGGTTTGGGACCAAGGGGTCGCAGGTTCAAATCCTGTCTTCCCGACCA", "db_xrefs": [ "SO:0000268" ], "anti_codon_pos": [ 257125, 257127 ], "id": "JPCNHPFPBM_2", "locus": "GCA000292505_00465" }, { "type": "tRNA", "sequence": "contig_1", "start": 257188, "stop": 257264, "strand": "+", "gene": "trnM", "product": "tRNA-Met(cat)", "amino_acid": "Met", "anti_codon": "cat", "score": 81.0, "nt": "GGCTGGATACCTCAGTTGGTTAGAGGGCCCGGTTCATACCCGGGTTGTCGTGAGTTCGAGTCTCACTCCAGCCACCA", "db_xrefs": [ "SO:0000266" ], "anti_codon_pos": [ 257222, 257224 ], "id": "JPCNHPFPBM_3", "locus": "GCA000292505_00466" }, { "type": "tRNA", "sequence": "contig_1", "start": 257284, "stop": 257360, "strand": "+", "gene": "trnI", "product": "tRNA-Ile2(cat)", "amino_acid": "Ile2", "anti_codon": "cat", "score": 74.3, "nt": "GGATCTATAGCTCAATTGGTTAGAGCCCCCGACTCATAATCGGTCTGTTACAGGTTCAAGTCCTGTTAGATCCACCA", "db_xrefs": [ "SO:0000263" ], "id": "JPCNHPFPBM_4", "locus": "GCA000292505_00467" }, { "type": "tRNA", "sequence": "contig_1", "start": 257398, "stop": 257489, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(tga)", "amino_acid": "Ser", "anti_codon": "tga", "score": 75.0, "nt": "GGAGACTTACCCAAGCGGCTGAAGGGTTCGGTCTTGAAAACCGAGAGGTGCTTTATAAGCACGCGAGGGTTCAAATCCCTCAGTCTCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 257417, 257419 ], "id": "JPCNHPFPBM_5", "locus": "GCA000292505_00468" }, { "type": "tRNA", "sequence": "contig_1", "start": 257503, "stop": 257579, "strand": "+", "gene": "trnfM", "product": "tRNA-fMet(cat)", "amino_acid": "fMet", "anti_codon": "cat", "score": 78.9, "nt": "CGCGGGATAGAGCAGTTGGTCAGCTCGTCAGGCTCATAATCTGAAGGTCGAGGGTTCAAATCCCTCTCCCGCAACCA", "db_xrefs": [ "SO:0000266" ], "id": "JPCNHPFPBM_6", "locus": "GCA000292505_00469" }, { "type": "tRNA", "sequence": "contig_1", "start": 257581, "stop": 257654, "strand": "+", "gene": "trnD", "product": "tRNA-Asp(gtc)", "amino_acid": "Asp", "anti_codon": "gtc", "score": 55.6, "nt": "GGTTCCATGGTGTAGTGATAACATATCTCCCTGTCACGGAGGGGTTGCGGGTTTGATTCCCGTTGGAACCGCCA", "db_xrefs": [ "SO:0000256" ], "anti_codon_pos": [ 257613, 257615 ], "id": "JPCNHPFPBM_7", "locus": "GCA000292505_00470" }, { "type": "tRNA", "sequence": "contig_1", "start": 257657, "stop": 257732, "strand": "+", "gene": "trnF", "product": "tRNA-Phe(gaa)", "amino_acid": "Phe", "anti_codon": "gaa", "score": 77.8, "nt": "GGTCTTGTAGCTCAGTCGGTAGAGCAACGGTCTGAAGAACCGTGTGTCGGCAGTTCGATTCTGCCCGAGACCACCA", "db_xrefs": [ "SO:0000267" ], "anti_codon_pos": [ 257690, 257692 ], "id": "JPCNHPFPBM_8", "locus": "GCA000292505_00471" }, { "type": "cds", "sequence": "contig_1", "start": 257880, "stop": 258998, "strand": "+", "frame": 3, "gene": null, "product": "cytadherence-associated protein P65", "db_xrefs": [ "BlastRules:WP_010869376", "BlastRules:WP_010874665", "RefSeq:WP_014894622.1", "SO:0001217", "UniParc:UPI00027B3896", "UniRef:UniRef100_UPI00027B3896", "UniRef:UniRef50_P47459", "UniRef:UniRef90_P47459" ], "nt": "ATGGAAAAAAATAGATCAGCTTTTCAACAAAACCAACAAGCATCAAACCAACCTTTTAACCAAGATCAAAACCAGTATTACCAAGATCCTAACCAACAACAATTTAACCAATCTGGTTTTGATCCAAATCAACAGCAATTTAATCAACCAGGATTTGATCCTAACCAACAGTATTACCAAGATCCCAATCAACAACAATTTAATCAATCAGGTTTTGATCCAAACCAACAATATTATCAAGATCCCAATCAACAACAATTTAATCAATCAGGTTTTGATCCAAACCAACAATATTATCAAGATCCCAATCAACAACAATTTAATCAATCAGGTTTTGATCAAAACCAGTATTATCAAGATCCCAATCAACAACAATTTAATCAATCAGGTTTTGATCAAAACCAGTACTATCAAGATCCTAATCAACAACAATTTAACCAGCCTAGTTTTGATCTAAATAACCAACAATTTAACCAACCTGGATTCAACCAATCCCCAGCATTTGAAATCACACCTCAAGAGCAAAAAGCTGAACAGGAGATGTTTGGTGAAGAACCACCTCAAGTAGTTAGAGAGATCCATGAACTACCATTTGAAAAGATCCGTTCTTTTTTACAAAGTGATTTTGACAGCTATAACTTTCGCTTAAACTCACTTAAAAGCAAACTGGATAATGCCTTGTATTCACTTGATAAAACAATCCAAAATACCAATGAAAACACTGCTAATCTAGAAGCAATTAGACATAACTTAGAACAAAAGATTCAAAACCAAAGCAAGCAATTAAGAACTAACTTTGATACCCAAAAGCTTGATGATAAGATCAATGAATTGGAGATCAGAATGCAAAAACTAACCAGGAATTTTGAATCTCTAAGTGAACTTTCAAAGCACAACTCTTATCCTAATTACTATGAAAAATTGTTACCAAATGGTGGTGATAGTATGACCAATGTCTTTGAAAAAGCACTAATGATGAATTTATTGAGAACTACATTACCCCCTCAACCCCAAGTTCAATACTACCCCCAACCCTATCCATACATAAGACCTTACTATGATGAACCTATTTACGCTGGGTTTAGAAGAAGGGGTTACCGTGATGACTTCTATGAATAA", "aa": "MEKNRSAFQQNQQASNQPFNQDQNQYYQDPNQQQFNQSGFDPNQQQFNQPGFDPNQQYYQDPNQQQFNQSGFDPNQQYYQDPNQQQFNQSGFDPNQQYYQDPNQQQFNQSGFDQNQYYQDPNQQQFNQSGFDQNQYYQDPNQQQFNQPSFDLNNQQFNQPGFNQSPAFEITPQEQKAEQEMFGEEPPQVVREIHELPFEKIRSFLQSDFDSYNFRLNSLKSKLDNALYSLDKTIQNTNENTANLEAIRHNLEQKIQNQSKQLRTNFDTQKLDDKINELEIRMQKLTRNFESLSELSKHNSYPNYYEKLLPNGGDSMTNVFEKALMMNLLRTTLPPQPQVQYYPQPYPYIRPYYDEPIYAGFRRRGYRDDFYE", "aa_hexdigest": "4a5774bc0576e590f6dba042146de2fd", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3896", "ncbi_nrp_id": "WP_014894622.1", "uniref100_id": "UniRef100_UPI00027B3896", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3896", "RefSeq:WP_014894622.1", "UniRef:UniRef100_UPI00027B3896" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3896", "uniref90_id": "UniRef90_P47459" }, "psc": { "uniref90_id": "UniRef90_P47459", "product": "Proline-rich P65 protein homolog", "uniref50_id": "UniRef50_P47459" }, "pscc": { "uniref50_id": "UniRef50_P47459", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47459" ], "product": "Proline-rich P65 protein homolog" }, "expert": [ { "type": "expert_proteins", "source": "BlastRules", "rank": 70, "id": "WP_010869376", "gene": null, "product": "cytadherence-associated protein P65", "query_cov": 1.0, "subject_cov": 1.0, "identity": 1.0, "score": 445.0, "evalue": 5.01e-157, "db_xrefs": [ "BlastRules:WP_010869376", "BlastRules:WP_010874665" ] } ], "genes": [], "id": "JPCNHPFPBM_499", "locus": "GCA000292505_00472" }, { "type": "cds", "sequence": "contig_1", "start": 258937, "stop": 259029, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGAACCTATTTACGCTGGGTTTAGAAGAAGGGGTTACCGTGATGACTTCTATGAATAAAAAGCGTGTTTTAACTAATGAAACCATTTGA", "aa": "MMNLFTLGLEEGVTVMTSMNKKRVLTNETI", "aa_hexdigest": "c7d305b1308506acc292e516438af4c2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3402.0336000000007, "isoelectric_point": 5.986078071594237 }, "id": "JPCNHPFPBM_500", "locus": "GCA000292505_00473" }, { "type": "cds", "sequence": "contig_1", "start": 259016, "stop": 262168, "strand": "+", "frame": 2, "gene": "hmw2", "product": "Cytadherence high molecular weight protein 2", "db_xrefs": [ "COG:COG1196", "COG:D", "GO:0020035", "SO:0001217", "UniRef:UniRef50_P75471", "UniRef:UniRef90_P47460" ], "nt": "ATGAAACCATTTGATAAAAAACCTTCGCTCCAACCAATTTATGACATTGGTTTTGATGATGGTTATCTCCAAAGTGAGTATGAAAAAAATCGTTCTAAAACCGATGTTGATAAGATCGAAAGCCAGCTTTTAAAAGAGATTAAAAGTCTGGAGGATGAACTTAAAAACCTCAAGGGCTTGAAGAATCAAGCAGAAGATAATCCTGAACTTGATAAAAAGATTAACCACTTGGAAGTTGATCTAAACCGTTTGGTTAATGAATATAAAAACTTCCAGTTCCAAAAGAACCACATGGTTGATAAGGTTAGTGAACTTGATAACCTAACCCGTTTTTATAAGAATGAACTAACCCGTTTGCAACAAGAAAACGCTGATTTTCTCAACTCCAAGTATGCTAATTTAGCTAACTTCCAAGCTAACTACCACAATAAACTAAATGATTTTCACCGCTTAATAGAAAATCAAAACCACACCATTAACCGCTTAAACCAAAAGATTAATGGTAACCAAAATCTGATTGATAATAACGTTGCTTTACTGCAAAACCCCAACATCACAGTTGAAAAAAAGAACTACTTACTAAATGTTATTGATCAACTTTACAATGAGCTTGATCAACTTGAGAATCAAAAAAGATTATTAAGTATTGAGTATGAAAATACCTATAGAGAGTTAGTTAGTGCAGATAATGAACTGCAAAATGTTTATGAAAACATCGATCAAAATCAGATCCAGTTTAAACACCAATACCAAACTTATAGAGATGAGTTAAGTCAACTTGAGCGCAAGATCCAGCTCACCAAACAAGAGTTAGTTGATAAAGAATCAGCACTAAGAGTAAAGATAGATGATGCTGATTTTTACATTAATGCCCGTTTAGCTGAACTTGATGATGTAGCTAAACAACTTAGCTTTCAAGATGGTATTACCAAGCAAAATGCTCAACATGTTGAGGATAAGTTAGTTGCTTTAAACAAAGAAAAAGACCGTTTAAATACCCAAAAAGAGGCCTTTTTTAACTTAAGACAATCTGCTTTAATTGATATCAATAAACTCCAGCAGGAAAATGAACTGTTTGCTAAGCACTTAGAACACCAGCAAAATGAGTTTGAACAAAAACAGTCTGATAGCCTCTTAAAGCTTGAAACTGAATATAAGGCTTTACAACACAAGATTAATGAATTTAAAAATGAAAGTGCCACTAAGAGCGAGGAACTTTTAAACCAAGAACGGGAACTATTTGAAAAACGCAGGGAAATTGACACGCTTTTAACCCAAGCATCTTTGGAATATGAACACCAACGTGAGTCAAGTCAACTACTCAAAGATAAGCAGAATGAAGTAAAACAACACTTCCAAAACTTAGAGTATGCTAAAAAGGAGCTAGACAAAGAAAGAAACCTCTTAGATCAACAAAAAAAAGTTGATAGTGAAGCAATCTTTCAACTCAAAGAAAAGGTTGCTCAAGAACGTAAGGAGCTTGAAGAACTATACCTTGTTAAAAAACAAAAACAAGATCAGAAGGAAAATGAACTGTTGTTTTTTGAAAAGCAGTTAAAACAACACCAAGCAGATTTTGAAAATGAATTGGAAGCTAAACAACAGGAGTTGTTTGAAGCTAAACATGCATTGGAACGTTCCTTTATCAAACTTGAAGATAAAGAAAAAGATCTTAACACTAAAGCACAACAGATTGCCAATGAGTTTTCCCAACTAAAAACTGATAAGTCAAAGAGTGCTGATTTTGAACTAATGTTGCAAAATGAGTATGAAAACTTGCAACAAGAAAAACAAAAGTTATTCCAAGAACGTACTTACTTTGAAAGGAATGCTGCGGTTTTATCAAACCGGTTACAACAAAAACGTGAGGAGTTATTACAACAAAAAGAAACGCTTGATCAGCTTACAAAAAGCTTTGAGCAAGAACGGTTAATCAACCAAAGGGAACACAAGGAGTTGGTTGCATCAGTTGAAAAACAAAAGGAGATATTGGGCAAAAAACTCCAAGATTTTTCCCAAACTTCACTGAACGCTTCTAAAAATTTAGCTGAACGGGAGATGGCAATCAAGTTTAAAGAAAAGGAGATAGAAGCAACTGAAAAGCAACTGTTAAATGATGTTAATAATGCTGAAGTTATCCAAGCAGACTTAGCACAACTCAACCAATCACTTAACCAAGAACGCAGTGAATTGCAAAACGCCAAACAAAGGATTGCTGATTTTCACAATGATTCACTAAAAAAACTCAATGAGTATGAACTTAGCTTACAAAAACGGTTGCAAGAATTACAAACCCTTGAGGTTAACCAAAAACAACATTCATATCAAAATCAAGCTTACTTTGAAGGTGAACTTGATAAACTTAACAGAGAAAAACAAGCTTTTTTGAACTTACGTAAGAAACAAACTATGGAGGTTGATGCTATTAAACAAAGGTTGAGTGATAAACATCAAGCTTTAAATATGCAACAAGCAGAGCTAGATAGAAAAACCCATGAGTTAAATAATGCTTTTTTAAACCATGATGCGGATCAAAAGAGTCTACAGGACCAACTAGCAACTGTTAAAGAGACCCAAAAACTAATTGATTTAGAACGTAGTGCACTGCTTGAAAAGCAACGTGAGTTTGCTGAAAATGTTGCTGGTTTTAAGCGCCATTGGTCTAATAAAACTAGTCAACTCCAAAAGATTTATGAACTGACCAAAAAACAGGAAAGTGAGCAAACCCAAAAGGAAACAGAACTAAAGATTGCTTTTAGTGATCTACAAAAAGACTATCAGGTTTTTGAACTCCAAAAGGACCAAGAATTTAGACAAATTGAAGCTAAGCAACGTGAACTTGACAAGTTAGCTGAAAAAAATAATCAGGTCAAACTAGAACTTGATAACAGGTTTCAAGCGCTGCAAAACCAAAAGCAAGACACAGTACAAGCTCAGCTAGAACTGGAACGTGAACAACACCAGTTAAACCTTGAGCAAACTGCTTTCAACCAAGCTAATGAATCGCTTTTAAAACAACGTGAACAACTCACCAAAAAGATCCAAGCTTTCCACTATGAGTTGAAAAAGCGTAACCAATTCTTAGCTTTAAAAGGGAAAAGGTTGTTTGCAAAAGAGCAAGATCAACAACGCAAAGATCAGGAGATCAACTGA", "aa": "MKPFDKKPSLQPIYDIGFDDGYLQSEYEKNRSKTDVDKIESQLLKEIKSLEDELKNLKGLKNQAEDNPELDKKINHLEVDLNRLVNEYKNFQFQKNHMVDKVSELDNLTRFYKNELTRLQQENADFLNSKYANLANFQANYHNKLNDFHRLIENQNHTINRLNQKINGNQNLIDNNVALLQNPNITVEKKNYLLNVIDQLYNELDQLENQKRLLSIEYENTYRELVSADNELQNVYENIDQNQIQFKHQYQTYRDELSQLERKIQLTKQELVDKESALRVKIDDADFYINARLAELDDVAKQLSFQDGITKQNAQHVEDKLVALNKEKDRLNTQKEAFFNLRQSALIDINKLQQENELFAKHLEHQQNEFEQKQSDSLLKLETEYKALQHKINEFKNESATKSEELLNQERELFEKRREIDTLLTQASLEYEHQRESSQLLKDKQNEVKQHFQNLEYAKKELDKERNLLDQQKKVDSEAIFQLKEKVAQERKELEELYLVKKQKQDQKENELLFFEKQLKQHQADFENELEAKQQELFEAKHALERSFIKLEDKEKDLNTKAQQIANEFSQLKTDKSKSADFELMLQNEYENLQQEKQKLFQERTYFERNAAVLSNRLQQKREELLQQKETLDQLTKSFEQERLINQREHKELVASVEKQKEILGKKLQDFSQTSLNASKNLAEREMAIKFKEKEIEATEKQLLNDVNNAEVIQADLAQLNQSLNQERSELQNAKQRIADFHNDSLKKLNEYELSLQKRLQELQTLEVNQKQHSYQNQAYFEGELDKLNREKQAFLNLRKKQTMEVDAIKQRLSDKHQALNMQQAELDRKTHELNNAFLNHDADQKSLQDQLATVKETQKLIDLERSALLEKQREFAENVAGFKRHWSNKTSQLQKIYELTKKQESEQTQKETELKIAFSDLQKDYQVFELQKDQEFRQIEAKQRELDKLAEKNNQVKLELDNRFQALQNQKQDTVQAQLELEREQHQLNLEQTAFNQANESLLKQREQLTKKIQAFHYELKKRNQFLALKGKRLFAKEQDQQRKDQEIN", "aa_hexdigest": "dce1241aa4efa52d6c58aa329f4d814c", "start_type": "ATG", "rbs_motif": null, "genes": [ "hmw2" ], "truncated": "3-prime", "pseudogene": { "start": 259016, "stop": 262471, "observations": { "mutation": [ 262166 ], "stop": [ 262166 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47460", "query_cov": 1.0, "subject_cov": 0.5817174515235457, "identity": 0.998, "score": 1572.0, "evalue": 0.0, "gene_start": 1, "gene_end": 1050, "reference_start": 1, "reference_end": 1050, "reference_sequence": "MKPFDKKPSLQPIYDIGFDDGYLQSEYEKNRSKTDVDKIENQLLKEIKSLEDELKNLKGLKNQAEDNPELDKKINHLEVDLNRLVNEYKNFQFQKNHMVDKVSELDNLTRFYKNELTRLQQENADFLNSKYANLANFQANYHNKLNDFHRLIENQNQTINRLNQKINGNQNLIDNNVALLQNPNITVEKKNYLLNVIDQLYNELDQLENQKRLLSIEYENTYRELVSADNELQNVYENIDQNQIQFKHQYQTYRDELSQLERKIQLTKQELVDKESALRVKIDDADFYINARLAELDDVAKQLSFQDGITKQNAQHVEDKLVALNKEKDRLNTQKEAFFNLRQSALIDINKLQQENELFAKHLEHQQNEFEQKQSDSLLKLETEYKALQHKINEFKNESATKSEELLNQERELFEKRREIDTLLTQASLEYEHQRESSQLLKDKQNEVKQHFQNLEYAKKELDKERNLLDQQKKVDSEAIFQLKEKVAQERKELEELYLVKKQKQDQKENELLFFEKQLKQHQADFENELEAKQQELFEAKHALERSFIKLEDKEKDLNTKAQQIANEFSQLKTDKSKSADFELMLQNEYENLQQEKQKLFQERTYFERNAAVLSNRLQQKREELLQQKETLDQLTKSFEQERLINQREHKELVASVEKQKEILGKKLQDFSQTSLNASKNLAEREMAIKFKEKEIEATEKQLLNDVNNAEVIQADLAQLNQSLNQERSELQNAKQRIADFHNDSLKKLNEYELSLQKRLQELQTLEANQKQHSYQNQAYFEGELDKLNREKQAFLNLRKKQTMEVDAIKQRLSDKHQALNMQQAELDRKTHELNNAFLNHDADQKSLQDQLATVKETQKLIDLERSALLEKQREFAENVAGFKRHWSNKTSQLQKIYELTKKQESEQTQKETELKIAFSDLQKDYQVFELQKDQEFRQIEAKQRELDKLAEKNNQVKLELDNRFQALQNQKQDTVQAQLELEREQHQLNLEQTAFNQANESLLKQREQLTKKIQAFHYELKKRNQFLALKGKRLFAKEQDQQRKDQEINWRFKQFEKEYTDFDEAKKRELEELEKIRRSLSQSNVELERKREKLATDFTNLNKVQHNTQINRDQLNSQIRQFLLERKNFQRFSNEANAKKAFLIKRLRSFASNLKLQKEALAIQKLEFDKRDEQQKKELQQATLQLEQFKFEKQNFDIEKQRQLVAIKTQCEKLSDEKKALNQKLVELKNLSQTYLANKNKAEYSQQQLQQKYTNLLDLKENLERTKDQLDKKHRSIFARLTKFANDLRFEKKQLLKAQRIVDDKNRLLKENERNLHFLSNETERKRAVLEDQISYFEKQRKQATDAILASHKEVKKKEGELQKLLVELETRKTKLNNDFAKFSRQREEFENQRLKLLELQKTLQTQTNSNNFKTKAIQEIENSYKRGMEELNFQKKEFDKNKSRLYEYFRKMRDEIERKESQVKLVLKETQRKANLLEAQANKLNIEKNTIDFKEKELKAFKDKVDQDIDSTNKQRKELNELLNENKLLQQSLIERERAINSKDSLLNKKIETIKRQLHDKEMRVLRLVDRMKLAEQKYQTEINRLRTQTFDSEKQDIKNFFPPLFKINGNDMAFPYLYPWLYPQQKQDDNTLQIRQLFEQQLQFMQQRYENELNELRRQRNLLEKKLDQIQLESQLNNKQSEFSKVESMMEKLLEKTESRLNDFDQKINYLTKKVNQHNTYQPSSYQPTPSYQDSDKQQLLFRIQELEKQNLFQQQFQPAPAVVQQPTSFAAPNITKQQQIAQLNAEINNIKRLIAQKAASK" }, "paralog": false, "identity": 0.9973935708079931, "score": 1903.0, "evalue": 0.0, "description": "Internal stop codon at 262166. Nonsense mutation around 262166.", "psc": { "uniref90_id": "UniRef90_P47460", "gene": "hmw2", "product": "Cytadherence high molecular weight protein 2", "uniref50_id": "UniRef50_P75471", "cog_id": "COG1196", "cog_category": "D", "go_ids": [ "GO:0020035" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47460", "UniRef:UniRef50_P75471", "COG:COG1196", "COG:D", "GO:0020035" ] }, "pscc": { "uniref50_id": "UniRef50_P75471", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75471" ], "product": "Cytadherence high molecular weight protein 2" } }, "id": "JPCNHPFPBM_501", "locus": "GCA000292505_00474" }, { "type": "cds", "sequence": "contig_1", "start": 262943, "stop": 263884, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGAAAATGAACGTAACCTCCATTTCCTTTCCAATGAAACAGAACGAAAACGAGCAGTTCTCGAAGATCAAATTTCTTACTTTGAAAAACAACGTAAACAAGCTACTGATGCGATCCTAGCATCACATAAAGAAGTTAAAAAGAAGGAAGGTGAACTGCAAAAGTTACTGGTTGAATTAGAAACAAGAAAAACCAAACTCAACAATGATTTTGCAAAATTCTCAAGACAACGTGAAGAGTTTGAAAACCAACGCTTAAAGCTCTTGGAACTGCAAAAAACCCTGCAAACCCAAACTAATTCCAACAACTTTAAAACCAAAGCAATCCAAGAGATTGAAAACAGTTATAAAAGGGGGATGGAAGAACTTAACTTCCAAAAGAAGGAGTTTGATAAGAATAAATCACGCTTATATGAATACTTTAGAAAGATGCGTGATGAGATTGAAAGAAAGGAAAGTCAGGTTAAGTTAGTTTTAAAAGAGACCCAAAGGAAAGCCAACCTCTTAGAAGCACAAGCCAACAAACTTAACATTGAAAAAAACACTATTGACTTTAAAGAAAAAGAGTTAAAAGCCTTTAAAGATAAGGTTGATCAAGACATTGATTCAACCAATAAACAACGCAAGGAGTTAAATGAGCTTTTAAATGAAAACAAGTTATTACAACAATCACTAATCGAAAGAGAAAGGGCTATTAATTCCAAAGATTCACTTCTAAATAAGAAGATAGAAACGATTAAACGCCAACTCCATGATAAGGAGATGAGGGTGTTGCGCTTGGTTGATAGAATGAAATTAGCAGAACAGAAATACCAAACAGAAATCAACCGCTTAAGAACCCAAACTTTCGATTCTGAAAAACAAGACATTAAAAACTTCTTCCCACCGTTATTTAAAATTAACGGTAATGATATGGCCTTTCCTTACTTATACCCCTGA", "aa": "MKENERNLHFLSNETERKRAVLEDQISYFEKQRKQATDAILASHKEVKKKEGELQKLLVELETRKTKLNNDFAKFSRQREEFENQRLKLLELQKTLQTQTNSNNFKTKAIQEIENSYKRGMEELNFQKKEFDKNKSRLYEYFRKMRDEIERKESQVKLVLKETQRKANLLEAQANKLNIEKNTIDFKEKELKAFKDKVDQDIDSTNKQRKELNELLNENKLLQQSLIERERAINSKDSLLNKKIETIKRQLHDKEMRVLRLVDRMKLAEQKYQTEINRLRTQTFDSEKQDIKNFFPPLFKINGNDMAFPYLYP", "aa_hexdigest": "b6ff1330878229225a030002b5dc376f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 37801.716900000036, "isoelectric_point": 9.508780479431152 }, "id": "JPCNHPFPBM_502", "locus": "GCA000292505_00475" }, { "type": "cds", "sequence": "contig_1", "start": 263957, "stop": 264433, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACAACGCTATGAAAATGAGTTAAATGAATTGCGTAGACAACGTAATTTACTTGAGAAAAAACTTGATCAAATCCAACTAGAATCCCAGCTTAACAATAAGCAAAGTGAGTTTAGTAAGGTGGAATCAATGATGGAAAAACTACTTGAAAAAACTGAGAGTAGGTTAAATGATTTTGATCAGAAAATTAACTATCTCACCAAGAAAGTTAACCAACACAACACCTATCAACCAAGTTCCTATCAACCAACGCCTTCTTATCAAGACAGTGATAAACAACAGCTGTTATTTAGAATCCAAGAACTGGAAAAACAAAACTTATTCCAACAACAATTTCAACCTGCACCAGCTGTTGTCCAACAACCTACCAGTTTTGCAGCCCCTAACATCACCAAACAACAGCAAATTGCCCAACTTAATGCTGAAATTAACAACATTAAAAGGTTGATTGCCCAAAAAGCAGCAAGTAAATAA", "aa": "MQQRYENELNELRRQRNLLEKKLDQIQLESQLNNKQSEFSKVESMMEKLLEKTESRLNDFDQKINYLTKKVNQHNTYQPSSYQPTPSYQDSDKQQLLFRIQELEKQNLFQQQFQPAPAVVQQPTSFAAPNITKQQQIAQLNAEINNIKRLIAQKAASK", "aa_hexdigest": "c866201a75b0dae332d05662976d194c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18683.82479999999, "isoelectric_point": 9.245685005187987 }, "id": "JPCNHPFPBM_503", "locus": "GCA000292505_00476" }, { "type": "cds", "sequence": "contig_1", "start": 264436, "stop": 264948, "strand": "+", "frame": 1, "gene": null, "product": "MG491 domain-containing protein", "db_xrefs": [ "RefSeq:WP_238532030.1", "SO:0001217", "UniParc:UPI0002D6119B", "UniRef:UniRef100_UPI0002D6119B", "UniRef:UniRef50_UPI0002D6119B", "UniRef:UniRef90_UPI0002D6119B" ], "nt": "ATGGTTAATAATGAATATCAACAACTAAACACTTTAGTTGAGAGTGATGATGAAGCGGATCTTGTGATTGCTAACCTAGTTAAACAACTCAATGAACTAAAGCAAATCCTTGTTTCACTAGACAATCAAGAAGCAAGTGCCACTGCAGTTACTGATAAAAAGGAAGAGGAATACAACCAAAACCAATCCAGTTTCCATAACTTCAGCAAAGAGACACTGCAAAAGCAAGCAAAGCGTGGTTTTCTTTTATTGGAACGCTGTTCGTTGGTTGGGTTACAACAACTAGAGTTGGAGTATGTTAATTTGTTGGGCAGAAGTTTTGATTCTTATCAACAAAAAACAGAACTTTTAAACAACTTAAAGGAGCTTGTTGATGAACATTTCAGTGATACTGAAAAAATTATCAATACCCTTGAAAAGATCTTTGATGTCATTGGCGGTAGTGAATATACCCCTGTCGTCAGCGCTTTAAAAAGTTATCACAAAAGATTGACAGTTCTCTCAAACAGATAG", "aa": "MVNNEYQQLNTLVESDDEADLVIANLVKQLNELKQILVSLDNQEASATAVTDKKEEEYNQNQSSFHNFSKETLQKQAKRGFLLLERCSLVGLQQLELEYVNLLGRSFDSYQQKTELLNNLKELVDEHFSDTEKIINTLEKIFDVIGGSEYTPVVSALKSYHKRLTVLSNR", "aa_hexdigest": "f4f4bc84c4b125659a5fb173a129c7ec", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002D6119B", "ncbi_nrp_id": "WP_238532030.1", "uniref100_id": "UniRef100_UPI0002D6119B", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002D6119B", "RefSeq:WP_238532030.1", "UniRef:UniRef100_UPI0002D6119B" ] }, "ips": { "uniref100_id": "UniRef100_UPI0002D6119B", "uniref90_id": "UniRef90_UPI0002D6119B" }, "psc": { "uniref90_id": "UniRef90_UPI0002D6119B", "product": "MG491 domain-containing protein", "uniref50_id": "UniRef50_UPI0002D6119B" }, "pscc": { "uniref50_id": "UniRef50_UPI0002D6119B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D6119B" ], "product": "MG491 domain-containing protein" }, "genes": [], "id": "JPCNHPFPBM_504", "locus": "GCA000292505_00477" }, { "type": "cds", "sequence": "contig_1", "start": 265080, "stop": 265388, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D5328C", "UniRef:UniRef90_UPI0002D5328C" ], "nt": "ATGGAAAATGAATTGGAAGCTGCTTTACTAGCAAAACACCAAGAACCACCGTTAATTGTTACCCTACCCAGCTTAATAAAACCAACTGTTAGTCAACCTGAAGTTGAAGTTGTTACACCTACTAACAACACTAATTTTCAACCCCAAGTTGATCTCAAACCTACTGATTTGAAAAAACAACACAAGAAAAAACCACTTAACTTTATTACCCGTCCTGTTTTCAAAAGTAATTTGCCACCAAAACTAAGTAAGGATGACATAGTTCATTATGCGCACCAGTTACTTGAAAAAAATACCCATAATGAATAG", "aa": "MENELEAALLAKHQEPPLIVTLPSLIKPTVSQPEVEVVTPTNNTNFQPQVDLKPTDLKKQHKKKPLNFITRPVFKSNLPPKLSKDDIVHYAHQLLEKNTHNE", "aa_hexdigest": "69699e8d651b30261ad6bebc86a64057", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 264996, "stop": 265388, "observations": { "mutation": [ 265044 ], "stop": [ 265044 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0002D5328C", "query_cov": 1.0, "subject_cov": 0.7846153846153846, "identity": 1.0, "score": 202.0, "evalue": 1.92e-65, "gene_start": 1, "gene_end": 102, "reference_start": 29, "reference_end": 130, "reference_sequence": "MFGPPDIANDHELNLNWPDSETDAILSSMENELEAALLAKHQEPPLIVTLPSLIKPTVSQPEVEVVTPTNNTNFQPQVDLKPTDLKKQHKKKPLNFITRPVFKSNLPPKLSKDDIVHYAHQLLEKNTHNE" }, "paralog": false, "identity": 0.9923076923076923, "score": 263.0, "evalue": 4.31e-92, "description": "Internal stop codon at 265044. Nonsense mutation around 265044.", "psc": { "uniref90_id": "UniRef90_UPI0002D5328C", "uniref50_id": "UniRef50_UPI0002D5328C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0002D5328C", "UniRef:UniRef50_UPI0002D5328C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0002D5328C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D5328C" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11632.27489999999, "isoelectric_point": 8.021108055114745 }, "id": "JPCNHPFPBM_505", "locus": "GCA000292505_00478" }, { "type": "cds", "sequence": "contig_1", "start": 265348, "stop": 265833, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MG219", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47461" ], "nt": "ATGCGCACCAGTTACTTGAAAAAAATACCCATAATGAATAGTGATAGTGATCTAAAACTCCAAAAGGTGTGGATCGAGCGGCATGTTGATCAAGATGAACTTAGTTTAACAACTACTGCAGTTGAACTTAAAAAGAGTGATGAACAAAAACCTGTTGCCATTAAAAGTAGTGACTTTATTGGCCATGAAGAATTAATCTCTGTTCCAGTTTTACTAATCCCAACCCCTGTTGTTAAAGAGATTGACCAACCAGTAGTTATTACTCCAGTTAAAGCAAAACCAAAAGCAACTAAAAAGAAAACTCCTGTTAAATCAAAACCAACTAATAAATCAACTAAACAAACAAAACCTAAACAACCCAAACCCAAATCAAAACAAGTTCAAAAAACCAAAGCTAAACCAAATCTTAATCTGGTTTTTTTAGTGTTAACAACAGTAGTTTTAACTAAAAACTGGAAAGGAATAGGAAGAACTACCTGGGGTTAA", "aa": "MRTSYLKKIPIMNSDSDLKLQKVWIERHVDQDELSLTTTAVELKKSDEQKPVAIKSSDFIGHEELISVPVLLIPTPVVKEIDQPVVITPVKAKPKATKKKTPVKSKPTNKSTKQTKPKQPKPKSKQVQKTKAKPNLNLVFLVLTTVVLTKNWKGIGRTTWG", "aa_hexdigest": "0a16649d577a3a6b054ab3a2d183e6ba", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00027B3BDF", "query_cov": 1.0062111801242235, "subject_cov": 1.0, "identity": 0.895, "score": 217.0, "evalue": 4.78e-70, "valid": false, "uniref50_id": "UniRef50_P47461" }, "pscc": { "uniref50_id": "UniRef50_P47461", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47461" ], "product": "Uncharacterized protein MG219" }, "genes": [], "id": "JPCNHPFPBM_506", "locus": "GCA000292505_00479" }, { "type": "cds", "sequence": "contig_1", "start": 265789, "stop": 265992, "strand": "-", "frame": 1, "gene": null, "product": "MPN313 family protein", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_Q49403", "UniRef:UniRef90_Q49403" ], "nt": "ATGATAGTTAATGCACTTACTGGGGTTGCACATGCAGTAAATGACATTTTTCAAAGTACCACAGCTAATGCCAATGGTTCTGATGATGATAATGAGAATAAAAATAATAGTTATCGCTCTAAAAGCAATTATTTTAACACCGCACGGTTTAAACTAGGATTAACCCCAGGTAGTTCTTCCTATTCCTTTCCAGTTTTTAGTTAA", "aa": "MIVNALTGVAHAVNDIFQSTTANANGSDDDNENKNNSYRSKSNYFNTARFKLGLTPGSSSYSFPVFS", "aa_hexdigest": "ef1995f1d2b9f9a0ae7f978eeed0a5e7", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 265789, "stop": 266070, "observations": { "mutation": [ 266016 ], "stop": [ 266016 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49403", "query_cov": 1.0, "subject_cov": 0.7204301075268817, "identity": 1.0, "score": 107.0, "evalue": 6.54e-29, "gene_start": 1, "gene_end": 67, "reference_start": 27, "reference_end": 93, "reference_sequence": "MYKLEKAQAKQVVGGLSFWTFSAGLIMIVNALTGVAHAVNDIFQSTTANANGSDDDNENKNNSYRSKSNYFNTARFKLGLTPGSSSYSFPVFS" }, "paralog": false, "identity": 0.989247311827957, "score": 154.0, "evalue": 2.08e-50, "description": "Internal stop codon at 266016. Nonsense mutation around 266016.", "psc": { "uniref90_id": "UniRef90_Q49403", "product": "MPN313 family protein", "uniref50_id": "UniRef50_Q49403", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49403", "UniRef:UniRef50_Q49403", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_Q49403", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49403" ], "product": "Uncharacterized protein MG220" } }, "id": "JPCNHPFPBM_507", "locus": "GCA000292505_00480" }, { "type": "tRNA", "sequence": "contig_1", "start": 266137, "stop": 266213, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(gcg)", "amino_acid": "Arg", "anti_codon": "gcg", "score": 65.2, "nt": "GTCATCATAGCTCAATAGGACAGAGTATCAGCTTGCGGAGCTGAGGGTTACAGGTTCGATTCCTGTTGGTGACGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 266171, 266173 ], "id": "JPCNHPFPBM_9", "locus": "GCA000292505_00481" }, { "type": "cds", "sequence": "contig_1", "start": 266341, "stop": 266805, "strand": "+", "frame": 1, "gene": "mraZ", "product": "division/cell wall cluster transcriptional repressor MraZ", "db_xrefs": [ "COG:COG2001", "COG:J", "GO:0000976", "GO:0003700", "GO:0005737", "GO:0006355", "GO:0009295", "GO:2000143", "SO:0001217", "UniRef:UniRef50_P47463", "UniRef:UniRef90_P47463" ], "nt": "GTGTTTAAAAGTGTCGCAAAGTGTGACAAAGTGGAAAAAATGCTGCTAGGTACCTTTAATCTTACCCTTGATAACAAGAACAGAATTAGCTTGCCAGCTAAGCTCCGTAGTTTCTTTGATAGCAGCATAGTTATTAACCGCGGCTTTGAAAACTGTTTGGAAATTAGAAAACCTGCAGACTTTGAGAGTTATTTTCAAACCTTTAATAACTTCCCTAACACCCAAAAAGACACAAGAACATTAAAACGCTTAATCTTTGCTAATGCTAATCTAGTTGAACTTGATAGTGCAAACAGAATCCTAATCCCTAATAACCTAATTAGTGATGCTAAGTTAGATAAAGAGATCGTGTTAATTGGTCAATTTGACCATCTTGAAGTTTGGGATAAAGTGCAATATGAACAATATCTAGCTAGTTCAGAATCACTAGAGACAGTAGCTGAAAGGATGAAAGATGCTAAATAA", "aa": "MFKSVAKCDKVEKMLLGTFNLTLDNKNRISLPAKLRSFFDSSIVINRGFENCLEIRKPADFESYFQTFNNFPNTQKDTRTLKRLIFANANLVELDSANRILIPNNLISDAKLDKEIVLIGQFDHLEVWDKVQYEQYLASSESLETVAERMKDAK", "aa_hexdigest": "e5c898d6258936bf40e8c63458aea833", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47463", "query_cov": 0.9155844155844156, "subject_cov": 1.0, "identity": 1.0, "score": 276.0, "evalue": 8.68e-94, "valid": true, "gene": "mraZ", "product": "division/cell wall cluster transcriptional repressor MraZ", "uniref50_id": "UniRef50_P47463", "cog_id": "COG2001", "cog_category": "J", "go_ids": [ "GO:0000976", "GO:0003700", "GO:0005737", "GO:0006355", "GO:0009295", "GO:2000143" ] }, "pscc": { "uniref50_id": "UniRef50_P47463", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47463" ], "product": "Transcriptional regulator MraZ" }, "genes": [ "mraZ" ], "id": "JPCNHPFPBM_508", "locus": "GCA000292505_00482" }, { "type": "cds", "sequence": "contig_1", "start": 266795, "stop": 267724, "strand": "+", "frame": 2, "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "db_xrefs": [ "COG:COG0275", "COG:J", "EC:2.1.1.199", "GO:0005737", "GO:0016434", "GO:0070475", "GO:0071424", "RefSeq:WP_014894625.1", "SO:0001217", "UniParc:UPI00027B3899", "UniRef:UniRef100_UPI00027B3899", "UniRef:UniRef50_P47464", "UniRef:UniRef90_P47464" ], "nt": "ATGCTAAATAACCAACAGATCCACCAGAGTGTACTGATCAATGAAGTGATCCATAACCTCAATATTAACCCTTGTGGTAACTATTTAGATCTAACTGCAGGGTTTGCAGGACACAGTCAAAAGATCTTAGAAAAACTAACAACAGGAACTTTAACAATTAATGATGTTGATAAAGAAAGTATTAATTTTTGCCAAAAGCTTTTTTTTAAAAACAACAACGTTGTTATTATTCACGATAACTTTGCTAACTTCCCAGTTCATCTTAAACAACTATCAATAACCAAGTTTGATGGGATCTTAATGGACCTTGGTGTATCAAGCCATCAACTCAACCAACCTAATCGCGGTTTTAGTTTTAAGAATGATGGACCGATTGACATGCGTATGGACCAATCCAATCAGAAAAATACCGCACTAACAGTTTTAAAAAACTTAACTGAACAAAAGTTAAGTCTAATCCTTAAAAAGTATGGTGATATTAAACACCCTAAACCAATTGCTATTGGATTGAAAAAAGCAGTTCAAACTGAAAAAAATCTTACCACAACTCAACTAGCAAAAGTGGTAAAAGAATGTGCTACTGGATTTGAAAAATACCAATCAAGAAACTATCTTGCCAAAGTTTTTCAAGCAATTAGGATCTATCTTAATGATGAGATTACTAATCTGAAAACTGCGTTAACTTTTATCCCTAATCTTTTAAAAAACAACAGCAGGTTTCTTGTGATTGTTTTTCACTCCATTGAAGAAAAAGTTGTAAGGAATTTCATTGCAAAACTAACCAGCTTTATCCAACCTGAAGCTCTACCCATTAAACTCACTCCTGCTTACCAGTTAATTACAAAAAAACCAATCCTACCTTCCCAAAAAGAACTTGAATTAAACCCGCGTTCGCGTAGTGCCAAACTCTTTGTTATCCAAAAAAACTAG", "aa": "MLNNQQIHQSVLINEVIHNLNINPCGNYLDLTAGFAGHSQKILEKLTTGTLTINDVDKESINFCQKLFFKNNNVVIIHDNFANFPVHLKQLSITKFDGILMDLGVSSHQLNQPNRGFSFKNDGPIDMRMDQSNQKNTALTVLKNLTEQKLSLILKKYGDIKHPKPIAIGLKKAVQTEKNLTTTQLAKVVKECATGFEKYQSRNYLAKVFQAIRIYLNDEITNLKTALTFIPNLLKNNSRFLVIVFHSIEEKVVRNFIAKLTSFIQPEALPIKLTPAYQLITKKPILPSQKELELNPRSRSAKLFVIQKN", "aa_hexdigest": "61d1a6666627ebee5049efe1c807e37b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3899", "ncbi_nrp_id": "WP_014894625.1", "uniref100_id": "UniRef100_UPI00027B3899", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3899", "RefSeq:WP_014894625.1", "UniRef:UniRef100_UPI00027B3899" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3899", "uniref90_id": "UniRef90_P47464" }, "psc": { "uniref90_id": "UniRef90_P47464", "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "ec_ids": [ "2.1.1.199" ], "uniref50_id": "UniRef50_P47464", "cog_id": "COG0275", "cog_category": "J", "go_ids": [ "GO:0005737", "GO:0016434", "GO:0070475", "GO:0071424" ] }, "pscc": { "uniref50_id": "UniRef50_P47464", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47464" ], "product": "Ribosomal RNA small subunit methyltransferase H" }, "genes": [ "rsmH" ], "id": "JPCNHPFPBM_509", "locus": "GCA000292505_00483" }, { "type": "cds", "sequence": "contig_1", "start": 267726, "stop": 268961, "strand": "+", "frame": 3, "gene": null, "product": "MPN316 family protein", "db_xrefs": [ "RefSeq:WP_010869381.1", "SO:0001217", "UniParc:UPI00001394A5", "UniRef:UniRef100_P47465", "UniRef:UniRef50_P47465", "UniRef:UniRef90_P47465" ], "nt": "ATGTACAAACCAAAAAATATTAACAGCGTATTAACCTTTTATAAGGATCAGATCCAACTGGTTGTTAGTGATGATCAAAACCAGTTCAACATCTTGTTTTACCAAACAATTGATAACGATGGCTTTTATTCAAAACAACAGTTGAAAAACAAACTAAGACTCAAGTTAGCATTAAACCAACTAGTTGATCAAGCTAACTATTTTCTTGGTTTTAAACTGGAAAAGGTAGTTGTTGTTCTCGCTGAACTGATTGATGATTTGAAGATCCATAATTTCAAGAGTGAGATCTTTTTTACTGGTTATGATTTTGATCATAAAGCGATGATTAAAAAAGAAAAACAACGCTTTTGTGAGCAAAATAACCAACTAACAGTTATGGATACAATGGTTTTAAACTACCATGATGTTATTAACAATAAGATCACCAAAAGCTTTGCATTTAACAAGAGCTATGTGGCTAATTTAGTGGCATATTCCTCTAAAAGTAACCTGATTGGTGAGTTGAAGTTCTTTTTAAAAAGAAACGTTAATCTTAAGGTTAAAAAAATTATTAGTCACCACTTGGCATTAGCCAACTCCTTAAGTAAGAAACAAAACAACATGTTTGTTTATTTAGGACAAAAAACTACTGAACTGATGCTATTTATGGACAATGCTTTAGTTGATGTTATTACCAACCAGTTTGGTAAAAACCACTTTATTGATATTCCAGCTAACCAGGAAAACAAACCACTGCTTGAGTTTTTAGTTGATAACACCACTAAGATTGGTGATTGTTATTCGCTTGGTATGACCTATACAGATGGTGATAGTTACAAAGAGATTAAGGCTTTGACTATTGGTGATTTAATGCAAACAGTTAGTGACAAGATCAAAACCTTAATTGATTTTATTAACAGTGGTTCTCTAACTTTTTTCAACAAGTTTAAAACCTTACCTAAGCTATTGTATTTTTATACAAGATCAAAACAAATTACCAACCTTTTTCAAGCTAATGTTGCGCTTATCAATCCCCAGTTTAAAACTGTTGATATTTATAAGAACAAGATCCAGTTTATTAGTGAAAACTACCTGTTAAGCTGTGAAGCGATTAGCCTGCAGATTACCAATAGAATCAAAAACCAAATTAGTTTTGATTTCACAAATGCTGATAATATTCAAAAACCTAAACCAAAAAAACACTTCATGATCTTATCAAAACACCTAACAAAGTTTGTCCAACGCTTGGTTAAATAA", "aa": "MYKPKNINSVLTFYKDQIQLVVSDDQNQFNILFYQTIDNDGFYSKQQLKNKLRLKLALNQLVDQANYFLGFKLEKVVVVLAELIDDLKIHNFKSEIFFTGYDFDHKAMIKKEKQRFCEQNNQLTVMDTMVLNYHDVINNKITKSFAFNKSYVANLVAYSSKSNLIGELKFFLKRNVNLKVKKIISHHLALANSLSKKQNNMFVYLGQKTTELMLFMDNALVDVITNQFGKNHFIDIPANQENKPLLEFLVDNTTKIGDCYSLGMTYTDGDSYKEIKALTIGDLMQTVSDKIKTLIDFINSGSLTFFNKFKTLPKLLYFYTRSKQITNLFQANVALINPQFKTVDIYKNKIQFISENYLLSCEAISLQITNRIKNQISFDFTNADNIQKPKPKKHFMILSKHLTKFVQRLVK", "aa_hexdigest": "9cd5851d12c857a80d991e52316963bc", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394A5", "ncbi_nrp_id": "WP_010869381.1", "uniref100_id": "UniRef100_P47465", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394A5", "RefSeq:WP_010869381.1", "UniRef:UniRef100_P47465" ] }, "ips": { "uniref100_id": "UniRef100_P47465", "uniref90_id": "UniRef90_P47465" }, "psc": { "uniref90_id": "UniRef90_P47465", "product": "MPN316 family protein", "uniref50_id": "UniRef50_P47465" }, "pscc": { "uniref50_id": "UniRef50_P47465", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47465" ], "product": "Uncharacterized protein MG223" }, "genes": [], "id": "JPCNHPFPBM_510", "locus": "GCA000292505_00484" }, { "type": "cds", "sequence": "contig_1", "start": 268964, "stop": 269854, "strand": "+", "frame": 2, "gene": "ftsZ", "product": "cell division protein FtsZ", "db_xrefs": [ "COG:COG0206", "COG:D", "GO:0000910", "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0032153", "GO:0043093", "GO:0051258", "GO:0051301", "KEGG:K03531", "SO:0001217", "UniRef:UniRef50_P47466", "UniRef:UniRef90_P47466" ], "nt": "ATGGATGAAAATGAAACTCAATTCAACAAGTTAAACCAAGTTAAAAACAAGCTGAAAATTGGTGTTTTTGGGATTGGAGGTGCTGGTAATAACATTGTTGATGCATCACTTTATCACTATCCTAATTTAGCAAGTGAAAACATCCACTTTTATGCTATAAATTCAGATTTACAGCACCTTGCATTTAAAACGAATGTTAAAAATAAACTCTTAATTCAAGACCATACTAACAAGGGCTTTGGAGCGGGGGGTGATCCAGCTAAAGGAGCTAGTTTAGCAATAAGCTTTCAAGAACAGTTTAATACACTTACAGATGGGTATGATTTTTGTATCTTAGTTGCTGGATTTGGTAAGGGTACTGGTACAGGTGCTACCCCAGTTTTTAGCAAGATCTTAAAAACTAAGAAGATCTTAAATGTTGCTATTGTTACCTATCCATCTTTAAACGAGGGATTAACAGTGAGAAACAAAGCAACTAAGGGACTTGAAATTCTCAACAAAGCAACTGATAGTTACATGCTATTTTGTAATGAAAAATGTACAAATGGTATCTACCAACTAGCAAACACAGAGATAGTCAATGCCATTAAAAACCTAATAGAACTAATTACTATTCCTTTGCAGCAAAACATTGATTTTGAAGATGTACGTGCCTTTTTTCAAACCAAAAAAACTAACCAAGATCAACAGCTTTTTACTGTTACTCACCCCTTTAGTTTTAGCTTTGATAGTAAAGATAGTATAGAACAGTTTGCCAAACAGTTTAAGAACTTTGAAAAAGTTAGTTATTTTGACCACTCTATAGTAGGAGCTAAAAAAGTGTTATTGAAAGCTAACATTAACCAAAAGATAGTCAAGCTTAATTTCAAGCAGATTCAAGATATTATCTGA", "aa": "MDENETQFNKLNQVKNKLKIGVFGIGGAGNNIVDASLYHYPNLASENIHFYAINSDLQHLAFKTNVKNKLLIQDHTNKGFGAGGDPAKGASLAISFQEQFNTLTDGYDFCILVAGFGKGTGTGATPVFSKILKTKKILNVAIVTYPSLNEGLTVRNKATKGLEILNKATDSYMLFCNEKCTNGIYQLANTEIVNAIKNLIELITIPLQQNIDFEDVRAFFQTKKTNQDQQLFTVTHPFSFSFDSKDSIEQFAKQFKNFEKVSYFDHSIVGAKKVLLKANINQKIVKLNFKQIQDII", "aa_hexdigest": "09741727f2cf9b0400dce48b33c9a132", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47466", "query_cov": 1.0, "subject_cov": 0.8021680216802168, "identity": 0.997, "score": 565.0, "evalue": 4.71e-202, "valid": true, "gene": "ftsZ", "product": "cell division protein FtsZ", "uniref50_id": "UniRef50_P47466", "cog_id": "COG0206", "cog_category": "D", "kegg_orthology_id": "K03531", "go_ids": [ "GO:0000910", "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0032153", "GO:0043093", "GO:0051258", "GO:0051301" ] }, "pscc": { "uniref50_id": "UniRef50_P47466", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47466" ], "product": "Cell division protein FtsZ" }, "genes": [ "ftsZ" ], "id": "JPCNHPFPBM_511", "locus": "GCA000292505_00485" }, { "type": "cds", "sequence": "contig_1", "start": 269903, "stop": 270073, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACAACCATCCCTAATATCCAAATTTTTATCCTCAGTGAACACAAAAATCCAGTTTCACTTCCCATTGATAATAAACCAACGGAAAACAACCAAAATAAGTTGAAACTTTTAGATGAACTGAAAGAACTTGGCATGAAATATGTTAAGCACCAAAACCAAATCTACTAA", "aa": "MTTIPNIQIFILSEHKNPVSLPIDNKPTENNQNKLKLLDELKELGMKYVKHQNQIY", "aa_hexdigest": "d4c1a75572535f6ce10bc62f5e837843", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6575.567200000001, "isoelectric_point": 8.050183296203613 }, "id": "JPCNHPFPBM_512", "locus": "GCA000292505_00486" }, { "type": "cds", "sequence": "contig_1", "start": 270119, "stop": 270406, "strand": "+", "frame": 2, "gene": null, "product": "amino acid permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027DFE8A8" ], "nt": "ATGGGTCAAATCAATCGGAAGTTTAGCGAAAAGCAGTTCTTACTTTTTGTTGTTAACTATATTGCTGGATTTGGCTTTATTGCTACTGCTATCTCACTGTTTCGCTTAGGACCTTTTTCTTGGTTAATCTTTCTGCTTGTTAGCTTAGTGAGTTTAATTGTTACCTTATCATTCGCACGGCTTTCATCAATAGATAGTCAAAACTATGGTGGGCCTTATCTTTGGGCTAAGAAAGCGGTTGATAAAGAGAAGATAGCAGGGAGAATGTTTAGCTTTTTTACTGGGTGA", "aa": "MGQINRKFSEKQFLLFVVNYIAGFGFIATAISLFRLGPFSWLIFLLVSLVSLIVTLSFARLSSIDSQNYGGPYLWAKKAVDKEKIAGRMFSFFTG", "aa_hexdigest": "9aa49a3a7eaf04b55bdcdf088691fde6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0027DFE8A8", "query_cov": 0.9263157894736842, "subject_cov": 1.0, "identity": 0.67, "score": 122.0, "evalue": 1.42e-34, "valid": false, "product": "amino acid permease", "uniref50_id": "UniRef50_UPI0027DFE8A8" }, "pscc": { "uniref50_id": "UniRef50_UPI0027DFE8A8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027DFE8A8" ], "product": "amino acid permease" }, "genes": [], "id": "JPCNHPFPBM_513", "locus": "GCA000292505_00487" }, { "type": "cds", "sequence": "contig_1", "start": 270334, "stop": 270507, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAACTAAGTTACCTCTAATCCCATCAATACCACTAAAGGAATTGAGGATAAAAAGTGGTGCAGTTGCTGCTGAAAGGGGACCAATGATAAAGTTATTTCACCCAGTAAAAAAGCTAAACATTCTCCCTGCTATCTTCTCTTTATCAACCGCTTTCTTAGCCCAAAGATAA", "aa": "MLTKLPLIPSIPLKELRIKSGAVAAERGPMIKLFHPVKKLNILPAIFSLSTAFLAQR", "aa_hexdigest": "dc4015c7b6b0a5f0d0550e2cd38aeccb", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6279.680499999999, "isoelectric_point": 11.165940284729004 }, "id": "JPCNHPFPBM_514", "locus": "GCA000292505_00488" }, { "type": "cds", "sequence": "contig_1", "start": 270611, "stop": 271588, "strand": "+", "frame": 2, "gene": null, "product": "APC family permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB1D5B2" ], "nt": "GTGAAGTGGATTGTGATCCTCTCAGCACTAATAGTAGCAATCTATGTTATTGCTAGAGATGGTAATGGTTATAGTAAAAACAATAACTTAGAAAGTGGTTTTTTTGGGAGAAGAGAGATTAGTTTTGCACAGATAGCAACGGTATTTATTACCTTCTTTTATTCTTATGCAGGGGTTGAAGATATCTCAGTGATGACTCCTGATGTTAAAACTAATAACTTTAGAAAGATATTAATTGTCTCTTTTATAGCAGTTTTCCTCTTTTATTTCATTGGGATTATTATTCTAAATGGTTTGCAAAACATTGCTCAAAGAGGTGGGGAAGCCAATTCAATTGGTAATGTAGCGGATATCTTTAAAAAAGCTGCTGGGCTTGGGACTTTAATCTTTTATGGAGTTGGAGCATTGTTTAACAATGTCTCAACCAGACTTTCAACTATTATTGCCAACTCCAGAAAGATTCTTCCGCTTGCTTATGATAACTATTTACCTAGTTTCTTTTACAAGCAAAACAAAAAAGGTGAGTTTCAGAATGCAATTTGGTTTACCTTTGGTACTACTTTAATTGCAATGACTTTGCTTGTCTTTATCCCTTTAGTTGCTTCTAACTTTGATTTTGATAATGCTACTGAGTATGCAGCATCTGTTGGCTCAGCTGCAACTTTGCTACAATATATTTTTGTTTTTTTTATAATCTTTAAGTTTATCTATAAAAAAGAACCACTCTACCAGAAAAAATGGGTTAAAACAACTGAAGAATTATTGTTTTGTTTAGGAACAATTGTCATTGTTTTAATGTTGTTGGTTTATCTGTTTCCTGTTATAGATGGATTTTCAAAATGGGAAACTAAACACACATTAACAATTGTGTTATATGGGGTTTTAAGCCTGATAGGATTGGTACTTTTTTTGCTCCAAGAATACAAACATAAAAACAAGCAAAATGCAAACAAACAAACAACCCAAACAACAGTTTAG", "aa": "MKWIVILSALIVAIYVIARDGNGYSKNNNLESGFFGRREISFAQIATVFITFFYSYAGVEDISVMTPDVKTNNFRKILIVSFIAVFLFYFIGIIILNGLQNIAQRGGEANSIGNVADIFKKAAGLGTLIFYGVGALFNNVSTRLSTIIANSRKILPLAYDNYLPSFFYKQNKKGEFQNAIWFTFGTTLIAMTLLVFIPLVASNFDFDNATEYAASVGSAATLLQYIFVFFIIFKFIYKKEPLYQKKWVKTTEELLFCLGTIVIVLMLLVYLFPVIDGFSKWETKHTLTIVLYGVLSLIGLVLFLLQEYKHKNKQNANKQTTQTTV", "aa_hexdigest": "441b225dc00698d06d4aed015e9697fc", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001FB1D5B2", "query_cov": 0.9723076923076923, "subject_cov": 0.8610354223433242, "identity": 0.69, "score": 428.0, "evalue": 1.43e-147, "valid": false, "product": "APC family permease", "uniref50_id": "UniRef50_UPI001FB1D5B2" }, "pscc": { "uniref50_id": "UniRef50_UPI001FB1D5B2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FB1D5B2" ], "product": "APC family permease" }, "genes": [], "id": "JPCNHPFPBM_515", "locus": "GCA000292505_00489" }, { "type": "cds", "sequence": "contig_1", "start": 271554, "stop": 272831, "strand": "+", "frame": 3, "gene": "potE", "product": "putative protein MG226", "db_xrefs": [ "COG:COG0531", "COG:E", "GO:0005886", "GO:0055085", "SO:0001217", "UniRef:UniRef50_P47468", "UniRef:UniRef90_P47468" ], "nt": "ATGCAAACAAACAAACAACCCAAACAACAGTTTAGTGAAAAGCAATTCATTGCTTTTGTCTTTAACTATATTGCTGGATTTGGCTTTATATCAGTGGTGATGACCATGTTTGATGTTGGGCCATTTTCCTATCTTGTTTTAGGGTTAACTTCGTTTGCTATTTTAGGAGTTGTGCTTTCTTTTTCTCGCCTTTCAGTTCTCTGTGGTAATAGTGCTTATGGAGGGAGTTATTTAATTGCTAAAAAAGCAGTTGGTACTAACAGTAAAACAAAAAGGTTTTTTGTTTTTTTAAGTGGGTGGAATGTATCGTTAACAGGATCTTTTAACGGTGTTGTTATTCCAGCAGTATTAATCTTTTCCTTTGCAGATATTCCAGTAGTTAAAGCGAATAATAACATCATTATTGGTCTTTTAGTAGGTGGATTTTTGTTGTTTGGCTTACTTACTTTTATCTCGTTATTTGGTTTAAAAATTAACAAGAAAGCAATCTTTTATTTTGCTGTTATTAAGTGGATAGTAGTAATAGGTGGGTTTATCTTAGGGATCTATTTAATTGGTACTACCAATGGTAAAGGGTTTGTTGAAAACAATTTAATTGGGACTAGGGAAAACATTGATTTTTTCAAGATTATCTTTATTAGTCTGGCTTTAACTATTGCTTTTGCAGGGACAGAGGATTTAGCTTCGATTACTCCTGATGTCAAGTCAAATAACTTAAGAAAGTGTTTTTTAATTGCCTTTGGGTGTGTTGTGTTACTTTACCTAGTTGGGTTTGTTATTATCAGTGGACTTGATGGGATTAGAGGTTATGGATTAGCATTAGGTAATAAAGATCCCAAGGCAATTAATAACTATGGATCTATCTACCGTTTGGTAGGAGGAGTTCCTTTACTTGTTATCTATGGACTTGGGTTACTTGTCAATTCCTTAGCATCACGCTTATCAATGACAATTACAACAGCTAGAAAATATGTAGCTTTAGCTCAAGATGGGTTTTTACCCTCTTTTTTAGCCAAAACTAATAAACATAATGAGTATCACCATGCAGTTTTAATTAGTAATCTAATGACTTTATTAGTGATGCTAATTATGGTAATAATCCCCTTTTTACCAGACCATAACAACAATAACAATAGTTTGTTTAATGCTATTGAACAGTTGGTTACAGTTACCATTGAAATGGCTGCAGCCATTTCTTTGATCCAATACTTTATTACCTTTATCTTCTTTTTTATGATCTTCGCTAAAAAGGAAAACCAGAAGTTAATTCCCTTGTGA", "aa": "MQTNKQPKQQFSEKQFIAFVFNYIAGFGFISVVMTMFDVGPFSYLVLGLTSFAILGVVLSFSRLSVLCGNSAYGGSYLIAKKAVGTNSKTKRFFVFLSGWNVSLTGSFNGVVIPAVLIFSFADIPVVKANNNIIIGLLVGGFLLFGLLTFISLFGLKINKKAIFYFAVIKWIVVIGGFILGIYLIGTTNGKGFVENNLIGTRENIDFFKIIFISLALTIAFAGTEDLASITPDVKSNNLRKCFLIAFGCVVLLYLVGFVIISGLDGIRGYGLALGNKDPKAINNYGSIYRLVGGVPLLVIYGLGLLVNSLASRLSMTITTARKYVALAQDGFLPSFLAKTNKHNEYHHAVLISNLMTLLVMLIMVIIPFLPDHNNNNNSLFNAIEQLVTVTIEMAAAISLIQYFITFIFFFMIFAKKENQKLIPL", "aa_hexdigest": "19bbd7d3277a58c4969b42ceb418756b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47468", "query_cov": 1.0, "subject_cov": 0.8638211382113821, "identity": 1.0, "score": 756.0, "evalue": 3.17e-273, "valid": true, "gene": "PotE", "product": "Uncharacterized protein MG226", "uniref50_id": "UniRef50_P47468", "cog_id": "COG0531", "cog_category": "E", "go_ids": [ "GO:0005886", "GO:0055085" ] }, "pscc": { "uniref50_id": "UniRef50_P47468", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47468" ], "product": "Uncharacterized protein MG226" }, "genes": [ "potE" ], "id": "JPCNHPFPBM_516", "locus": "GCA000292505_00490" }, { "type": "cds", "sequence": "contig_1", "start": 273012, "stop": 273176, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013052C28" ], "nt": "GTGATCATTGATTTAACCCCAACATTGATGTTAGAAAAAGTGGGGAGTGGGGTATTTGAACTAACAGAAAGGGTAGCAGTGTGGGAGTTGGAGAAGGGTTTGTTGTTGCCGATTCAAAAGCAATTTCGCTTAATGAACTGTAGATTAGCTATTACTATTCATTAA", "aa": "MIIDLTPTLMLEKVGSGVFELTERVAVWELEKGLLLPIQKQFRLMNCRLAITIH", "aa_hexdigest": "dab7f975beff291864d8d38981f67232", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0013052C28", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.815, "score": 82.0, "evalue": 1.09e-19, "valid": false, "uniref50_id": "UniRef50_UPI0013052C28" }, "pscc": { "uniref50_id": "UniRef50_UPI0013052C28", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013052C28" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6209.456399999997, "isoelectric_point": 6.538439369201661 }, "id": "JPCNHPFPBM_517", "locus": "GCA000292505_00491" }, { "type": "cds", "sequence": "contig_1", "start": 273177, "stop": 273410, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTAAATGGGTGTATGTAGTTTTTTTGGAGGAGCCACCAGCTGGGGTGACTTTGTTTTCCATGGAGGAGGGATTAAACGAAGGACTACTACTACTACTACTAGGCAACACACTTGTCAAGGAGATGCCGTTGGTGTTTGATGAACCCTTGCGATACCCAGCCCCAGTTCAAATGTCTGGTGAAGAGTTAGGGGTAAACACCCACCGGGTGTTTTCTTTGTTTAAGTGTTGA", "aa": "MGKWVYVVFLEEPPAGVTLFSMEEGLNEGLLLLLLGNTLVKEMPLVFDEPLRYPAPVQMSGEELGVNTHRVFSLFKC", "aa_hexdigest": "4073c41ecbdc513418a22a6753f8ec04", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8641.080599999996, "isoelectric_point": 4.597899436950684 }, "id": "JPCNHPFPBM_518", "locus": "GCA000292505_00492" }, { "type": "cds", "sequence": "contig_1", "start": 273508, "stop": 273690, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00303.24" ], "nt": "ATGAAACAGTATTTAGATTTAGCTAGTTATGTTTTAGCAAATGGTAAAAAAAGAAAAAACCGTACAGATACAGATACTTTAAGTGTCTTTGGTTACCAGATGAAATTTGACCTTACTAATAGTTTTCCTTTATTGACAACTAAAAAGGTTAATTGGAAGGCAATTGTCCATGAATTGTTGTGA", "aa": "MKQYLDLASYVLANGKKRKNRTDTDTLSVFGYQMKFDLTNSFPLLTTKKVNWKAIVHELL", "aa_hexdigest": "bb6b9ca0c6ac04d4b0d6b8e779825ef8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0037C09292", "query_cov": 1.0, "subject_cov": 0.9375, "identity": 0.6, "score": 75.1, "evalue": 1.01e-16, "valid": false, "product": "thymidylate synthase", "uniref50_id": "UniRef50_A0A967H814" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00303.24", "name": "Thymidylat_synt", "length": 60, "aa_cov": 0.9833333333333333, "hmm_cov": 1.0, "evalue": 7.298407044176557e-23, "score": 76.10872650146484, "start": 2, "stop": 60 } ], "seq_stats": { "molecular_weight": 6995.086499999999, "isoelectric_point": 9.755372047424316 }, "id": "JPCNHPFPBM_519", "locus": "GCA000292505_00493" }, { "type": "cds", "sequence": "contig_1", "start": 274000, "stop": 274371, "strand": "+", "frame": 1, "gene": "thyA", "product": "thymidylate synthase", "db_xrefs": [ "COG:COG0207", "COG:F", "EC:2.1.1.45", "GO:0004799", "GO:0005829", "GO:0006231", "GO:0006235", "GO:0032259", "KEGG:K00560", "SO:0001217", "UniRef:UniRef50_P47469", "UniRef:UniRef90_P47469" ], "nt": "ATGGCATTGGCTCCTTGTCATTCACTCTTTCAGTTCTATGTTGAAGAAGATAAACTAAGCTTACAGCTTTACCAGCGCAGCGGTGATATCTTTCTTGGTGTCCCATTTAACATTGCATCTTACGCCTTACTTGTGTATTTAGTTGCCCATGAAACTAAGTTAAAACCTGGTTATTTTATCCATACACTAGGAGATGCACATATCTATGAAAACCACATTGAACAAATTAAATTACAACTAACAAGAACAACCCTAGACCCCCCTCAAGTGGTTTTGAAAAGTGATAAATCAATCTTTGCTTATAGTTTTGATGATATTGAGTTAGTTGGTTATAATTACCATCCATTTATCTATGGGAGGGTTGCAGTTTAA", "aa": "MALAPCHSLFQFYVEEDKLSLQLYQRSGDIFLGVPFNIASYALLVYLVAHETKLKPGYFIHTLGDAHIYENHIEQIKLQLTRTTLDPPQVVLKSDKSIFAYSFDDIELVGYNYHPFIYGRVAV", "aa_hexdigest": "45fc626ecca00e240ef3748f49293db2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00260828A0", "query_cov": 1.008130081300813, "subject_cov": 0.8104575163398693, "identity": 0.629, "score": 156.0, "evalue": 1.25e-46, "valid": false, "gene": "thyA", "product": "thymidylate synthase", "ec_ids": [ "2.1.1.45" ], "uniref50_id": "UniRef50_A0A059X7H5", "kegg_orthology_id": "K00560", "go_ids": [ "GO:0004799", "GO:0006231" ] }, "genes": [ "thyA" ], "truncated": "5-prime", "pseudogene": { "start": 273700, "stop": 274371, "observations": { "mutation": [ 273745, 273754, 273886, 273976 ], "stop": [ 273745, 273754, 273886, 273976 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47469", "query_cov": 1.0, "subject_cov": 0.42857142857142855, "identity": 1.0, "score": 254.0, "evalue": 2.18e-83, "gene_start": 1, "gene_end": 123, "reference_start": 165, "reference_end": 287, "reference_sequence": "MKQYLDLASYVLANGKKRKNRTDTDTLSVFGYQMKFDLTNSFPLLTTKKVNWKAIVHELLWFIKGDTNIKYLVDNGVNIWNEWPYENFKKSPSFQNETLQEFILKVKTDNEFAKQFADLGPVYGKQWRNFNGVDQLKKVIQEIKENPNSRRLIVSSWNPSELEKMALAPCHSLFQFYVEEDKLSLQLYQRSGDIFLGVPFNIASYALLVYLVAHETKLKPGYFIHTLGDAHIYENHIEQIKLQLTRTTLDPPQVVLKSDKSIFAYSFDDIELVGYNYHPFIYGRVAV" }, "paralog": false, "identity": 0.9820627802690582, "score": 437.0, "evalue": 4.37e-158, "description": "Internal stop codon at 273745, 273754, 273886, 273976. Nonsense mutation around 273745, 273754, 273886, 273976.", "psc": { "uniref90_id": "UniRef90_P47469", "gene": "thyA", "product": "thymidylate synthase", "ec_ids": [ "2.1.1.45" ], "uniref50_id": "UniRef50_P47469", "cog_id": "COG0207", "cog_category": "F", "kegg_orthology_id": "K00560", "go_ids": [ "GO:0004799", "GO:0005829", "GO:0006231", "GO:0006235", "GO:0032259" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47469", "EC:2.1.1.45", "UniRef:UniRef50_P47469", "COG:COG0207", "COG:F", "KEGG:K00560", "GO:0004799", "GO:0005829", "GO:0006231", "GO:0006235", "GO:0032259" ] }, "pscc": { "uniref50_id": "UniRef50_P47469", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47469" ], "product": "Thymidylate synthase" } }, "id": "JPCNHPFPBM_520", "locus": "GCA000292505_00494" }, { "type": "cds", "sequence": "contig_1", "start": 274392, "stop": 274622, "strand": "+", "frame": 3, "gene": "folA", "product": "Dihydrofolate reductase", "db_xrefs": [ "COG:COG0262", "COG:H", "EC:1.5.1.3", "GO:0004146", "GO:0005829", "GO:0006730", "GO:0046452", "GO:0046654", "GO:0046655", "GO:0050661", "KEGG:K00287", "SO:0001217", "UniRef:UniRef50_P78028", "UniRef:UniRef90_P47470" ], "nt": "ATGACACAAGAAGGACTAATAGGTAATAACAACACTTTACCTTGGATGATTAAACAAGAGCTAGCTCACTTTAAAAAAACTACGTTATTTCAAGCTTTGTTAATGGGGAGAAAAACTTACGAATCACTCCCCAAGGTATTTGAAAAAAGAACAATATTTCTCCTTTCAAAAGATCAAAACTACCGTTTTGAAGAAAAGGGAAGTGAAGTGAAAGTTATTAATGATTTTTGA", "aa": "MTQEGLIGNNNTLPWMIKQELAHFKKTTLFQALLMGRKTYESLPKVFEKRTIFLLSKDQNYRFEEKGSEVKVINDF", "aa_hexdigest": "85ac8e532b79faa315a436b16ff85a8f", "start_type": "ATG", "rbs_motif": null, "genes": [ "folA" ], "truncated": "both", "pseudogene": { "start": 274371, "stop": 274853, "observations": { "mutation": [ 274386, 274620 ], "stop": [ 274386, 274620 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47470", "query_cov": 1.0, "subject_cov": 0.475, "identity": 1.0, "score": 154.0, "evalue": 1.32e-46, "gene_start": 1, "gene_end": 76, "reference_start": 8, "reference_end": 83, "reference_sequence": "MLIAIWAMTQEGLIGNNNTLPWMIKQELAHFKKTTLFQALLMGRKTYESLPKVFEKRTIFLLSKDQNYRFEEKGSEVKVINDFWPLIKSYQANKEKDLFICGGKSVYEQTINECDQLIVSIIKKKYKGDQFLKVDLSKFVLNEVVEFEEFNVNYYRKKQQ" }, "paralog": false, "identity": 0.9875, "score": 313.0, "evalue": 5.68e-112, "description": "Internal stop codon at 274386, 274620. Nonsense mutation around 274386, 274620.", "psc": { "uniref90_id": "UniRef90_P47470", "gene": "folA", "product": "Dihydrofolate reductase", "ec_ids": [ "1.5.1.3" ], "uniref50_id": "UniRef50_P78028", "cog_id": "COG0262", "cog_category": "H", "kegg_orthology_id": "K00287", "go_ids": [ "GO:0005829", "GO:0004146", "GO:0050661", "GO:0046452", "GO:0046655", "GO:0006730", "GO:0046654" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47470", "EC:1.5.1.3", "UniRef:UniRef50_P78028", "COG:COG0262", "COG:H", "KEGG:K00287", "GO:0005829", "GO:0004146", "GO:0050661", "GO:0046452", "GO:0046655", "GO:0006730", "GO:0046654" ] }, "pscc": { "uniref50_id": "UniRef50_P78028", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78028" ], "product": "Dihydrofolate reductase" } }, "id": "JPCNHPFPBM_521", "locus": "GCA000292505_00495" }, { "type": "cds", "sequence": "contig_1", "start": 274662, "stop": 274853, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00186.24" ], "nt": "TTGTTTATTTGTGGTGGAAAAAGTGTGTATGAACAGACCATTAATGAATGTGATCAGTTAATTGTTTCAATCATTAAAAAGAAGTATAAGGGTGATCAGTTTTTGAAGGTTGATCTCAGTAAATTTGTACTTAATGAAGTTGTAGAGTTTGAGGAATTTAATGTTAATTATTATAGAAAGAAACAACAATAA", "aa": "MFICGGKSVYEQTINECDQLIVSIIKKKYKGDQFLKVDLSKFVLNEVVEFEEFNVNYYRKKQQ", "aa_hexdigest": "158254ec2552fe2e19ebf56e2f50e11b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00186.24", "name": "DHFR_1", "length": 53, "aa_cov": 0.8253968253968254, "hmm_cov": 1.0, "evalue": 7.82856622462892e-10, "score": 33.77817916870117, "start": 1, "stop": 52 } ], "seq_stats": { "molecular_weight": 7516.623, "isoelectric_point": 7.714665412902832 }, "id": "JPCNHPFPBM_522", "locus": "GCA000292505_00496" }, { "type": "cds", "sequence": "contig_1", "start": 274859, "stop": 274990, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGCTAACAATAAAAAGTACTTTTTAGAATCATTTTCCCCACTTGGGTATGTAAAGAATAATTTTCAGGGCAACTTACGTTCTGTAAACTGGAATTTGGTCGATGATGAGAAGGATTTGGAAGTGTGA", "aa": "MAANNKKYFLESFSPLGYVKNNFQGNLRSVNWNLVDDEKDLEV", "aa_hexdigest": "a7567c754e2a481a928e9a97b07baeff", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5008.533000000001, "isoelectric_point": 4.992987251281736 }, "id": "JPCNHPFPBM_523", "locus": "GCA000292505_00497" }, { "type": "cds", "sequence": "contig_1", "start": 275210, "stop": 275881, "strand": "+", "frame": 2, "gene": null, "product": "Ribonucleoside-diphosphate reductase subunit beta", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2I1IMZ3" ], "nt": "ATGGTAGGGGTACATGCCCGTTCCTATGGAACGATCTTCTCAACTTTATGTACATCAGAACAGATTAACGCTGCTCATGAGTGGGTTGTAAACACTGAAAGTCTCCAGAAAAGAGCAAAGGCATTAATCCCTTACTATACGGGAAATGACCCGTTAAAATCAAAGGTAGCAGCAGCTTTAATGCCTGGGTTTTTACTGTATGGTGGGTTTTATTTGCCTTTTTACTTGTCATCAAGAAAACAACTACCAAATACATCTGATATTATCCGCTTAATCCTTCGTGATAAAGTGATCCATAACTATTACAGTGGTTATAAATACCAACGTAAACTAGAAAAACTCCCTTTAGCAAAACAAAAGGAGATGAAAGCATTTGTTTTTGAACTAATGTATCGGTTAATTGAACTTGAAAAGAACTATTTAAAAGAGCTTTATGAAGGGTTTGGAATTGTTGATGATGCCATTAAGTTCAGTGTTTACAATGCTGGTAAGTTTTTACAGAACTTAGGTTATGACTCCCCGTTTACTGCAGCAGAAACCAGGATTAAACCAGAGATTTTTGCCCAACTATCAGCACGTGCTGATGAAAACCATGACTTTTTCTCAGGAAACGGTTCGTCGTATGTGATGGGAGTTAGTGAAGAGACAAATGATGATGATTGGAACTTTTAA", "aa": "MVGVHARSYGTIFSTLCTSEQINAAHEWVVNTESLQKRAKALIPYYTGNDPLKSKVAAALMPGFLLYGGFYLPFYLSSRKQLPNTSDIIRLILRDKVIHNYYSGYKYQRKLEKLPLAKQKEMKAFVFELMYRLIELEKNYLKELYEGFGIVDDAIKFSVYNAGKFLQNLGYDSPFTAAETRIKPEIFAQLSARADENHDFFSGNGSSYVMGVSEETNDDDWNF", "aa_hexdigest": "46d555f0e608e4cec52f684e09619769", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2X3CV73", "query_cov": 1.0, "subject_cov": 0.8383458646616542, "identity": 0.507, "score": 230.0, "evalue": 1.02e-72, "valid": false, "product": "ribonucleoside-diphosphate reductase", "uniref50_id": "UniRef50_A0A2I1IMZ3" }, "pscc": { "uniref50_id": "UniRef50_A0A2I1IMZ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2I1IMZ3" ], "product": "Ribonucleoside-diphosphate reductase subunit beta" }, "genes": [], "id": "JPCNHPFPBM_524", "locus": "GCA000292505_00498" }, { "type": "cds", "sequence": "contig_1", "start": 275885, "stop": 276346, "strand": "+", "frame": 2, "gene": "nrdI", "product": "class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI", "db_xrefs": [ "COG:COG1780", "COG:F", "GO:0009263", "GO:0010181", "GO:0036211", "KEGG:K03647", "RefSeq:WP_014894231.1", "SO:0001217", "UniParc:UPI00027B389F", "UniRef:UniRef100_UPI00027B389F", "UniRef:UniRef50_P47472", "UniRef:UniRef90_P47472" ], "nt": "ATGCATAAAGATATCAAACTAGTTAAGGAAACTGAAATTAGAAAACCAATTGGTTCTCCTTTTATTGTCTATTTTTCATCTATCTCCAACAACACCCACCGTTTTATTGAAAAACTAGGTTTTCAACACAAAAGAATCCCAGTTGATATAACCCAAAGCATTACTATAAGTAATGAGTATGTTTTAATCTGTCCAACTTACAGTGGTGGGGGTAACCAGGTTGAAGGAGCGGTACCCAAGCAAGTTATCCAGTTTTTAAATAACAAGCATAACAGGGAGTTATGCAGAGGAGTTATTGCATCTGGTAACACTAATTTTGGAGATACTTTTTGTCTTGCAGGAACTGTTATTTCCAAAAAACTAAACGTCCCTTTGTTATATCAGTTTGAACTTTTGGGAACAAAAAATGATGTAGAACAAACTCAAAAAATAATTGCCAATTTCTTTCAAAACAGCAACTAG", "aa": "MHKDIKLVKETEIRKPIGSPFIVYFSSISNNTHRFIEKLGFQHKRIPVDITQSITISNEYVLICPTYSGGGNQVEGAVPKQVIQFLNNKHNRELCRGVIASGNTNFGDTFCLAGTVISKKLNVPLLYQFELLGTKNDVEQTQKIIANFFQNSN", "aa_hexdigest": "c3f79c7970add0d0004d7767c9a1bd86", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B389F", "ncbi_nrp_id": "WP_014894231.1", "uniref100_id": "UniRef100_UPI00027B389F", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B389F", "RefSeq:WP_014894231.1", "UniRef:UniRef100_UPI00027B389F" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B389F", "uniref90_id": "UniRef90_P47472" }, "psc": { "uniref90_id": "UniRef90_P47472", "gene": "nrdI", "product": "class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI", "uniref50_id": "UniRef50_P47472", "cog_id": "COG1780", "cog_category": "F", "kegg_orthology_id": "K03647", "go_ids": [ "GO:0009263", "GO:0010181", "GO:0036211" ] }, "pscc": { "uniref50_id": "UniRef50_P47472", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47472" ], "product": "Protein NrdI" }, "genes": [ "nrdI" ], "id": "JPCNHPFPBM_525", "locus": "GCA000292505_00499" }, { "type": "cds", "sequence": "contig_1", "start": 276365, "stop": 277993, "strand": "+", "frame": 2, "gene": null, "product": "class 1b ribonucleoside-diphosphate reductase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AFF10F" ], "nt": "ATGACATCCAAAGAAAAAATCCCTACTTTTAATACTGAAGAAGATGTTGAAAGTTACATTTCTTTTAATGCCCAAGCCAAAATCTATGATGATTTTGCAATCGATTTACAAGCAGTTGAAAGCTATATTCAAGAGCATGTAAAACCCAAAACCAAGGTCTTTCATTCCACCAAAGAACGCCTTGATTTTCTGATTAAGAACGATTATTATGATGAGAAGATCATCAACATGTACAGTTTTGAACAGTTTGAAGAGATCACCCATAAAGCATATTCATACCGCTTTCGTTATGCTAACTTCATGGGAGCATTTAAGTTCTATAATGCCTATGCTTTAAAGACATTTGATGGTAAGTACTACTTGGAAAACTATGAGGATAGGGTGGTGATGAATGTATTGATGTTAGCTAATGGCAACTTCAATAAGGCATTAAAACTCTTAAAACAGATTATCCTTAACCGTTTTCAACCAGCAACCCCTACCTTTCTTAATGCTGGTAGAAAGAAACGTGGTGAATTTGTTTCATGTTACCTGTTAAGGATTGAAGATAACATGGAATCAATAGGTAGAGCGATAACAACTACACTACAACTATCAAAACGTGATGGGGGAGTAGCACTTTTGCTTTCCAACTTACGTGAAGCGGGAGCGCCCATCAAAAAGATAGAAAACCAATCATCAGGGATTATCCCAATTATGAAATTGTTAGAGGACTCTTTTTCCTATTCCAACCAACTTGGACAAAGACAAGGAGCGGGAGCGGTGTATCTCCATTGTCACCATCCTGATGTTATGCAGTTTTTAGATACTAAAAGGGAAAATGCTGATGAGAAGATCAGAATTAAATCACTCTCCTTAGGACTTGTGATTCCAGATATCACCTTCCAATTAGCAAAAAATAACGAGATGATGGCACTTTTCAGTCCATATGATATCTATCAGGAGTATGGTAAGGCTTTATCTGATATCTCAGTAACTGAGATGTATTATGAATTGCTTGAAAACCAACGCATTAAAAAGACCTTTATTAGTGCTAGAAAGTTCTTTCAAACAATTGCTGAACTCCACTTTGAAAGTGGTTATCCCTACATCTTGTTTGATGATACAGTTAACAGGAGAAATGCCCACAAAAACAGGATAGTAATGTCTAACCTTTGCAGTGAAATTGTCCAACCATCTTTACCTTCTGAATTCTATTCAGACCTTACTTTTAAAAAGGTAGGTAGTGATATTAGCTGTAACTTGGGGAGTTTAAATATTGCTAGAGCAATGGAAAGTGGTAGTGAGTTAGCTGAATTGATTCAACTAGCAATTGAATCACTGGATTTAGTGTCAAGGATCAGTAGTTTAGAAACCGCTCCTTCCATTAAAAAAGGTAATTCAGAAAACCATGCGTTGGGATTAGGAGCGATGAACTTACATGGATTTTTAGCAACAAATGCTATCTATTATGATTCAAAGGAAGCGGTTGATTTTACTAACATCTTTTTTTATACAGTAGCATACCATGCGTTTAGTGCTTCCAATAAATTAGCATTGGAACTAGGTAAATTTAAAGACTTTGAAAATACTAAATTTGCTGATGGTAGTTACTTTGATAAGTACACTAAGGTAGCTAGTGACTTTTGA", "aa": "MTSKEKIPTFNTEEDVESYISFNAQAKIYDDFAIDLQAVESYIQEHVKPKTKVFHSTKERLDFLIKNDYYDEKIINMYSFEQFEEITHKAYSYRFRYANFMGAFKFYNAYALKTFDGKYYLENYEDRVVMNVLMLANGNFNKALKLLKQIILNRFQPATPTFLNAGRKKRGEFVSCYLLRIEDNMESIGRAITTTLQLSKRDGGVALLLSNLREAGAPIKKIENQSSGIIPIMKLLEDSFSYSNQLGQRQGAGAVYLHCHHPDVMQFLDTKRENADEKIRIKSLSLGLVIPDITFQLAKNNEMMALFSPYDIYQEYGKALSDISVTEMYYELLENQRIKKTFISARKFFQTIAELHFESGYPYILFDDTVNRRNAHKNRIVMSNLCSEIVQPSLPSEFYSDLTFKKVGSDISCNLGSLNIARAMESGSELAELIQLAIESLDLVSRISSLETAPSIKKGNSENHALGLGAMNLHGFLATNAIYYDSKEAVDFTNIFFYTVAYHAFSASNKLALELGKFKDFENTKFADGSYFDKYTKVASDF", "aa_hexdigest": "631bb5a82b2d3b009845328d30d3072b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001C049A88", "query_cov": 0.9022140221402214, "subject_cov": 0.9683168316831683, "identity": 0.6890000000000001, "score": 687.0, "evalue": 8.28e-244, "valid": false, "product": "ribonucleoside-diphosphate reductase subunit alpha", "ec_ids": [ "1.17.4.1" ], "uniref50_id": "UniRef50_UPI0021AFF10F", "go_ids": [ "GO:0004748", "GO:0005971", "GO:0009265" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0021AFF10F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021AFF10F" ], "product": "class 1b ribonucleoside-diphosphate reductase subunit alpha" }, "genes": [], "id": "JPCNHPFPBM_526", "locus": "GCA000292505_00500" }, { "type": "cds", "sequence": "contig_1", "start": 278072, "stop": 278530, "strand": "+", "frame": 2, "gene": null, "product": "Ribonucleotide-diphosphate reductase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A655JDN6" ], "nt": "TTGGTAGCAAGTATTCAAAAAGATGGACTTGCAAACTCCCATTTAATGGCTATTGCCCCAACTGGATCTATCTCATATCTCTCTTCATGTACCCCTTCACTTCAACCAGTAGTATCTCCTGTTGAAGTGAGAAAAGAAGGGAAGTTAGGACGGATTTATGTCCCTGCTTATAAGCTTGATAATGATAACTATCAGTACTTTAAAGATGGTGCTTATGAACTGGGCTTTGAACCTATTATTAACATAGTAGCAGCAGCCCAACAACATGTTGATCAAGCAATCTCTTTAACCTTGTTTATGACTGATAAAGCTACCACCAGAGATCTCAATAAAGCTTATATTTATGCTTTTAAAAAGGGTTGTAGTTCTATCTATTATGTCAGAGTAAGACAAGATGTTTTAAAAGATAGTGAAGATCACACTATTAAAATCAAGGATTGTGAGGTTTGTTCTATCTAA", "aa": "MVASIQKDGLANSHLMAIAPTGSISYLSSCTPSLQPVVSPVEVRKEGKLGRIYVPAYKLDNDNYQYFKDGAYELGFEPIINIVAAAQQHVDQAISLTLFMTDKATTRDLNKAYIYAFKKGCSSIYYVRVRQDVLKDSEDHTIKIKDCEVCSI", "aa_hexdigest": "ed87e14e2ff7b0324ee5d6ca5ad0f03d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A655JDN6", "query_cov": 0.9736842105263158, "subject_cov": 1.0422535211267605, "identity": 0.52, "score": 157.0, "evalue": 6.68e-47, "valid": false, "product": "Ribonucleotide-diphosphate reductase subunit alpha", "uniref50_id": "UniRef50_A0A655JDN6" }, "pscc": { "uniref50_id": "UniRef50_A0A655JDN6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A655JDN6" ], "product": "Ribonucleotide-diphosphate reductase subunit alpha" }, "genes": [], "id": "JPCNHPFPBM_527", "locus": "GCA000292505_00501" }, { "type": "cds", "sequence": "contig_1", "start": 278623, "stop": 278925, "strand": "+", "frame": 1, "gene": "rplU", "product": "50S ribosomal protein L21", "db_xrefs": [ "COG:COG0261", "COG:J", "GO:0003723", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02888", "RefSeq:WP_009885769.1", "SO:0001217", "UniParc:UPI0000133D46", "UniRef:UniRef100_P47474", "UniRef:UniRef50_P47474", "UniRef:UniRef90_P47474" ], "nt": "ATGCATGCTATTGTGGTTTGTGGTGCTAAGCAGTATTTAGTCCATGAAAACGAGTCTATTTTTGTTGAAAAATTAGCTGGTAAAGTTGGTCAGGAGATCCAACTTGATAAGGTATTGATGCTTGATGAAAAGATAGGCAAACCTTACCTTGAAAAAGCTAAGGTTGTTTGTGTGATTGAAAAACACGGTTTAAAATCGAAAATTAAACTAATTAAACACATCTCCCAAAAACACCACCTCAAGCGTTATGGCCACCGTCAACCCTACACCAAACTAAAAGTGGTACGCTTTATCCATGATTAA", "aa": "MHAIVVCGAKQYLVHENESIFVEKLAGKVGQEIQLDKVLMLDEKIGKPYLEKAKVVCVIEKHGLKSKIKLIKHISQKHHLKRYGHRQPYTKLKVVRFIHD", "aa_hexdigest": "3e7656c95e5048e12de9eb4e3e4b4a27", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133D46", "ncbi_nrp_id": "WP_009885769.1", "uniref100_id": "UniRef100_P47474", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133D46", "RefSeq:WP_009885769.1", "UniRef:UniRef100_P47474" ] }, "ips": { "uniref100_id": "UniRef100_P47474", "uniref90_id": "UniRef90_P47474" }, "psc": { "uniref90_id": "UniRef90_P47474", "gene": "rplU", "product": "50S ribosomal protein L21", "uniref50_id": "UniRef50_P47474", "cog_id": "COG0261", "cog_category": "J", "kegg_orthology_id": "K02888", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P47474", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47474" ], "product": "Large ribosomal subunit protein bL21" }, "genes": [ "rplU" ], "id": "JPCNHPFPBM_528", "locus": "GCA000292505_00502" }, { "type": "cds", "sequence": "contig_1", "start": 278918, "stop": 279217, "strand": "+", "frame": 2, "gene": "prp", "product": "Ribosomal processing cysteine protease Prp", "db_xrefs": [ "COG:COG2868", "COG:S", "EC:3.4.22.-", "GO:0006508", "GO:0008234", "GO:0042254", "KEGG:K07584", "RefSeq:WP_009885770.1", "SO:0001217", "UniParc:UPI00001394CA", "UniRef:UniRef100_P47475", "UniRef:UniRef50_P47475", "UniRef:UniRef90_P47475" ], "nt": "ATGATTAAGATTAATATCTCCCAAAACTTTCTAGTTGCAAAAGGTCATGCTTTGTTTGCTGAGAAGGGTAAGGACATAGTTTGTGCTGCAATTAGTGGAATTATCTTTGGGGGGGTGGCTTGGTTTGAACCTGATAAGATTGAATTTACTGAAAATAAATTAGTACCTAGTATAGCACTGAAACTCATTGACCCAACCCCTAATGTAGCAGTTGCTTTTAGTGTTATTACAGTACAATTAAAAGCAATAGCCAATTCCTATCCTAATCACATAGTTATCAATGAAGAGAGTTATGAGTAA", "aa": "MIKINISQNFLVAKGHALFAEKGKDIVCAAISGIIFGGVAWFEPDKIEFTENKLVPSIALKLIDPTPNVAVAFSVITVQLKAIANSYPNHIVINEESYE", "aa_hexdigest": "6ec548ab6a67574f06e1d557cff348cd", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394CA", "ncbi_nrp_id": "WP_009885770.1", "uniref100_id": "UniRef100_P47475", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394CA", "RefSeq:WP_009885770.1", "UniRef:UniRef100_P47475" ] }, "ips": { "uniref100_id": "UniRef100_P47475", "uniref90_id": "UniRef90_P47475" }, "psc": { "uniref90_id": "UniRef90_P47475", "gene": "prp", "product": "Ribosomal processing cysteine protease Prp", "ec_ids": [ "3.4.22.-" ], "uniref50_id": "UniRef50_P47475", "cog_id": "COG2868", "cog_category": "S", "kegg_orthology_id": "K07584", "go_ids": [ "GO:0008234", "GO:0006508", "GO:0042254" ] }, "pscc": { "uniref50_id": "UniRef50_P47475", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47475" ], "product": "Ribosomal processing cysteine protease Prp" }, "genes": [ "prp" ], "id": "JPCNHPFPBM_529", "locus": "GCA000292505_00503" }, { "type": "cds", "sequence": "contig_1", "start": 279210, "stop": 279524, "strand": "+", "frame": 3, "gene": "rpmA", "product": "50S ribosomal protein L27", "db_xrefs": [ "COG:COG0211", "COG:J", "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02899", "RefSeq:WP_009885771.1", "SO:0001217", "UniParc:UPI0000133E08", "UniRef:UniRef100_P47476", "UniRef:UniRef50_P05657", "UniRef:UniRef90_P47476" ], "nt": "ATGAGTAAAAACAGTTACTGCTACCAAATTAACTTACAGTTTTTCGCTTCCAAAAAAGGGGTTGGTTCCACTAAGAATGGACGTGATTCACATTCTAAGCGCTTGGGCGCTAAGAAGGCAGATGGTCAGATGATTAGAGTTGGTCAGATTATCTATAGACAAAGGGGAACTAAGATCTTTGCAGGACAAAATGTTGCAATGGGTAGTGATAACACCCTCTTTGCTTTGAGCGATGGCATTGTCCGCTTTCAAAAGTTTGGTAGTAAACAAAGCAAAACCCGGGTTAACATCATCAAACACCAACTAAATGCCTAA", "aa": "MSKNSYCYQINLQFFASKKGVGSTKNGRDSHSKRLGAKKADGQMIRVGQIIYRQRGTKIFAGQNVAMGSDNTLFALSDGIVRFQKFGSKQSKTRVNIIKHQLNA", "aa_hexdigest": "a68e3703ca3015bb7367831e969166d2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133E08", "ncbi_nrp_id": "WP_009885771.1", "uniref100_id": "UniRef100_P47476", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133E08", "RefSeq:WP_009885771.1", "UniRef:UniRef100_P47476" ] }, "ips": { "uniref100_id": "UniRef100_P47476", "uniref90_id": "UniRef90_P47476" }, "psc": { "uniref90_id": "UniRef90_P47476", "gene": "rpmA", "product": "50S ribosomal protein L27", "uniref50_id": "UniRef50_P05657", "cog_id": "COG0211", "cog_category": "J", "kegg_orthology_id": "K02899", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P05657", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P05657" ], "product": "Large ribosomal subunit protein bL27" }, "genes": [ "rpmA" ], "id": "JPCNHPFPBM_530", "locus": "GCA000292505_00504" }, { "type": "cds", "sequence": "contig_1", "start": 279517, "stop": 280362, "strand": "+", "frame": 1, "gene": "nfo", "product": "putative endonuclease 4", "db_xrefs": [ "COG:COG0648", "COG:L", "EC:3.1.21.2", "GO:0003677", "GO:0003906", "GO:0006284", "GO:0008081", "GO:0008270", "GO:0008833", "SO:0001217", "UniRef:UniRef50_P47477", "UniRef:UniRef90_P47477" ], "nt": "ATGCCTAAGCTACTGGGTAGCTTTATTAGCTTTAAAGCCCCCAATTACTTTGTTCAAAGTGCTCAGGATGCAATTGCTATTGATGCAACTGCTCTAATGGTATTTTTAGGTCCACCCCATTCTGCTTACCGTGTTCCTTTTAACAAGATGCAGTTTAGTTTGGGCTATGAGTTGTTAAAAACAAAGAATATTAATAGTAATGGTTTGGTTGTTCATGCTCCATATATCATTAACTGTGCATCAAAAGACCCACTAAAACAGCAGAATGCTATCAGTGTTTTAACCAATGAGATTCAGCTTTGTAACTTGGCTGGTGCTCACTATTTAGTTTTGCATCCAGGTTCTGCAGTAGCCCAAACAACCAACGAAGCATTAGATAACCTGGTTAAAGTACTCAATCAAGTTATCAATAAAACCAAAACAACAGTTATTTGTCTTGAAACAATGGCTGGTAAGGGTAATGAGATAGGCAGAGACTTAACTGAGTTGAAATATGTTATTGACAGGATCGTTGATAAAGATAGGATCGGGGTTTGTCTTGATACCTGTCACTTCCATGATAGTGGGATTGACTTTAGTGATCTAACTGGTGTTTTTAACACCATTACAACTAAACTTGGTTTTGAGTTTCTCAAAGTAATCCATTTAAATGAATCTAAAAATAATTGTGGTTCTAAAAAAGATAGGCATGCTAATATCAATGCTGGGATGATTGGTTTTGAGAACTTAATGAAGTTCATTAGTCACCCCCAAATTAAGGATTTACCTATTATCTTAGAAACCCCTTCAACTAGTTTAAACTACCCAACTATTTACCGTGAAGAGATTAGTCAAATCCGCAGCTGA", "aa": "MPKLLGSFISFKAPNYFVQSAQDAIAIDATALMVFLGPPHSAYRVPFNKMQFSLGYELLKTKNINSNGLVVHAPYIINCASKDPLKQQNAISVLTNEIQLCNLAGAHYLVLHPGSAVAQTTNEALDNLVKVLNQVINKTKTTVICLETMAGKGNEIGRDLTELKYVIDRIVDKDRIGVCLDTCHFHDSGIDFSDLTGVFNTITTKLGFEFLKVIHLNESKNNCGSKKDRHANINAGMIGFENLMKFISHPQIKDLPIILETPSTSLNYPTIYREEISQIRS", "aa_hexdigest": "1ff46dd14e955ac826d0954a7552843b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47477", "query_cov": 1.0, "subject_cov": 0.9656357388316151, "identity": 1.0, "score": 560.0, "evalue": 1.4e-201, "valid": true, "gene": "nfo", "product": "Probable endonuclease 4", "ec_ids": [ "3.1.21.2" ], "uniref50_id": "UniRef50_P47477", "cog_id": "COG0648", "cog_category": "L", "go_ids": [ "GO:0008833", "GO:0003677", "GO:0003906", "GO:0008081", "GO:0008270", "GO:0006284" ] }, "pscc": { "uniref50_id": "UniRef50_P47477", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47477" ], "product": "Probable endonuclease 4" }, "genes": [ "nfo" ], "id": "JPCNHPFPBM_531", "locus": "GCA000292505_00505" }, { "type": "cds", "sequence": "contig_1", "start": 280382, "stop": 280804, "strand": "+", "frame": 2, "gene": "fur", "product": "putative protein MG236", "db_xrefs": [ "COG:COG0735", "COG:P", "GO:0000976", "GO:0003700", "GO:0008270", "GO:0045892", "GO:1900376", "RefSeq:WP_009885773.1", "SO:0001217", "UniParc:UPI00001394D0", "UniRef:UniRef100_P47478", "UniRef:UniRef50_P75456", "UniRef:UniRef90_P47478" ], "nt": "ATGCTAACTAGTTATGTGAAGGTATTAGAACAAAACAATCTGCGCTTAACAAAACCGCGGATTGCTTTATTAAAGTGTTTAATTGAACACCAAGATTGGCATAATCTCTCCCAAATTAAAACCCACCTTGATTTAGCCAATCAACCCTCAACACTCGCTTCTATCTACAACAACTTAAGAATCCTAGCTAAACTTAAACTGATTAACATCTTTGTAGATCCAGAGCGGTTTGAAACTTACTATTGCCTGCGCCATGCAGAGCATAACCATATCTATCTTTTTGATGAAGTTAAACAGCAGTTTTTTACCTTACCTTTAACAGATGGCCAAATTAAAACATTGCTTGAAACCCAAAACCATACCAGTAAGGTAAAGCTTAATGATTTTTATATTGTTGCTAGGGGTGAGATAAACAATGATTAA", "aa": "MLTSYVKVLEQNNLRLTKPRIALLKCLIEHQDWHNLSQIKTHLDLANQPSTLASIYNNLRILAKLKLINIFVDPERFETYYCLRHAEHNHIYLFDEVKQQFFTLPLTDGQIKTLLETQNHTSKVKLNDFYIVARGEINND", "aa_hexdigest": "d36eeb029ffe79c931482dba3a03810b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394D0", "ncbi_nrp_id": "WP_009885773.1", "uniref100_id": "UniRef100_P47478", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394D0", "RefSeq:WP_009885773.1", "UniRef:UniRef100_P47478" ] }, "ips": { "uniref100_id": "UniRef100_P47478", "uniref90_id": "UniRef90_P47478" }, "psc": { "uniref90_id": "UniRef90_P47478", "gene": "Fur", "product": "Uncharacterized protein MG236", "uniref50_id": "UniRef50_P75456", "cog_id": "COG0735", "cog_category": "P", "go_ids": [ "GO:0003700", "GO:0000976", "GO:0008270", "GO:0045892", "GO:1900376" ] }, "pscc": { "uniref50_id": "UniRef50_P75456", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75456" ], "product": "Uncharacterized protein MG236 homolog" }, "genes": [ "fur" ], "id": "JPCNHPFPBM_532", "locus": "GCA000292505_00506" }, { "type": "cds", "sequence": "contig_1", "start": 280797, "stop": 281681, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MG237", "db_xrefs": [ "RefSeq:WP_009885774.1", "SO:0001217", "UniParc:UPI00001394D3", "UniRef:UniRef100_P47479", "UniRef:UniRef50_P75455", "UniRef:UniRef90_P47479" ], "nt": "ATGATTAACAAACCAAACCAATTTTTAAACCACCTTGATGGCTTAAAGCAACATTTTTCTGATTATGATTCACTACAAAAATCCTTTAAGAAATATCTATCAGAAAATCAAACTGAACTTAACAATTTCTTTTTTAACCAGTTTGAAAAGATCATTGTTCTGGTCAAAAAAAAGGAGTTTAAAACTGCTCAAGAGAGGTGTGAAGAGGAGTTAGCTACCCCTTATTTTTCAAAGCCATTGGTTGGTTTTTTCCAATCACTATTACAACTAATTAACCATGATCTCATCGAACAGAAAAACCAACAGTTAGCTAACATGAGTTGTGAAAAAATTGTGGAGATGGTGTTAAGTGATTACCCCAATAAACTTAACTTAATCCACTATTTGTTAGCAAAAGAAGCGAGTTTTGTAAACCCTAACCTTTTACAGCGGATGACTTTTGTGTTAACTGATCTTGAACTGTTAGAGTTAAAGCGTTTCTCTTTTTTTAAAGCCCTTAACCAGATACCTGCTTTTAAAAACCACAAAGTAACATACTTTAACAGCAAACTCAAACAGAAGTTTGTAATAACATTAGGTGAATTTGCTTTCCCCCAAACTGATAAAACCAAACAGTTTTTCCAACAACTAATTAAAAAAGTAAGTCAACTGTTTTTAAAAGAACCTGTTAGTTGTGAATTTGCTTATGAAATTATTGATGCATTACTCGTCAGTTTTTTTCCACTCCATCCTAATTTAGAAGTAAACCACTTAGCTAAAAAGATCCACCAGTATGTTAGTAAGATTGTCATTAATGAAGTTGTTGATCTGAAAGATCCAACCACTAAACTAATTGTTGATACACTTTATGAACAGTTAGATAGAGCAATTGGTGAGGAAAATTAA", "aa": "MINKPNQFLNHLDGLKQHFSDYDSLQKSFKKYLSENQTELNNFFFNQFEKIIVLVKKKEFKTAQERCEEELATPYFSKPLVGFFQSLLQLINHDLIEQKNQQLANMSCEKIVEMVLSDYPNKLNLIHYLLAKEASFVNPNLLQRMTFVLTDLELLELKRFSFFKALNQIPAFKNHKVTYFNSKLKQKFVITLGEFAFPQTDKTKQFFQQLIKKVSQLFLKEPVSCEFAYEIIDALLVSFFPLHPNLEVNHLAKKIHQYVSKIVINEVVDLKDPTTKLIVDTLYEQLDRAIGEEN", "aa_hexdigest": "3ddd0b764b47994e1c3f34780f7355db", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394D3", "ncbi_nrp_id": "WP_009885774.1", "uniref100_id": "UniRef100_P47479", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394D3", "RefSeq:WP_009885774.1", "UniRef:UniRef100_P47479" ] }, "ips": { "uniref100_id": "UniRef100_P47479", "uniref90_id": "UniRef90_P47479" }, "psc": { "uniref90_id": "UniRef90_P47479", "product": "Uncharacterized protein MG237", "uniref50_id": "UniRef50_P75455" }, "pscc": { "uniref50_id": "UniRef50_P75455", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75455" ], "product": "Uncharacterized protein MG237 homolog" }, "genes": [], "id": "JPCNHPFPBM_533", "locus": "GCA000292505_00507" }, { "type": "cds", "sequence": "contig_1", "start": 281822, "stop": 283045, "strand": "+", "frame": 2, "gene": "tig", "product": "trigger factor", "db_xrefs": [ "COG:COG0544", "COG:O", "EC:5.2.1.8", "GO:0003755", "GO:0005737", "GO:0005854", "GO:0015031", "GO:0043022", "GO:0043335", "GO:0044183", "GO:0051082", "GO:0051083", "GO:0051301", "GO:0061077", "GO:0140839", "GO:0140840", "SO:0001217", "UniRef:UniRef50_P47480", "UniRef:UniRef90_P47480" ], "nt": "ATGGCTAAATCGATAAAAATTAAGGGTTTTCGTCCCGGTAAAATCCCCCCTAATTTAGCCAGTCAGTCGATTAATAAAGCTGAATTAATGCAAAAAAGTGCCCAAAACGTCATGAACAGTATTTATGAATCAGTTCAACAAGAAGAGATCGTTGCTAGTAATGATAATGTCATTGATGATTATCCTACCATTGATTTCAAAACGATCACTGAACAAAACTGTGTACTTTTGTTTTACTTTGATCTGATCCCTAACTTTCAACTCCCTGATTACAAAAAGATAAAAGATTTAACACCACTTACCAAGTTAACTGAAGCTGAATTTAACAACGAAATTGAAAAGCTGGCAAAAACTAAAAGCACAATGGTAGATGTTAGTGATAAAAAACTAGCTAATGGTGATATTGCTATCATTGATTTCACTGGGATAGTTGATAACAAAAAACTAGCATCAGCTTCAGCACAAAACTATGAATTGACAATTGGTTCAAATAGCTTTATTAAGGGTTTTGAAACCGGGTTAATAGCAATGAAAGTTAACCAGAAAAAAACTTTAGCACTAACTTTTCCTAGTGATTATCATGTTAAGGAGTTGCAATCAAAACCAGTTACATTTGAAGTAGTTTTAAAAGCAATTAAAAAACTGGAATTCACCCCAATGGATGAAACTAATTTCAAATCCTTTCTCCCTGAACAATTCCAAAGCTTTACTTCTCTAAAGGCATTTAAGAGTTATTTTCATAAGCTAATGGAAAACAAAAAACAAGAGACAATTCTCCAGGAGAATAACCAAAAAATTCGTCAGTTCTTACTTACTAATACCAAACTTCCTTTTCTTCCAGAAGCGTTAATTAAACTAGAAGCTAACCGCTTGTTAAAGCTCCAGCAAAGCCAAGCTGAACAATATAAAATCCCCTTTGAAAAACTCTTAAGTGCTTCTAATATCACCCTAACAGAGTTACAAGATCGCAACATAAAAGAAGCTAAGGAAAATGTTACCTTTGCTTTGGTAATGAAAAAGATAGCTGACATTGAAAAGATTAAGGTTGATAATAACAAGATTAAAGCTGAAATTGAAAATGTTATTGCTGTTGAATATCCCTTTGCTAGTGATGAAATGAAAAAACAACTCTTTTTTAATATGGAACAACAAAAGGAGTTTGTGGAATCAATTATCATCAACAGATTAACAACAGCTAAAATCGTTAGCTATTCAACTCATTAG", "aa": "MAKSIKIKGFRPGKIPPNLASQSINKAELMQKSAQNVMNSIYESVQQEEIVASNDNVIDDYPTIDFKTITEQNCVLLFYFDLIPNFQLPDYKKIKDLTPLTKLTEAEFNNEIEKLAKTKSTMVDVSDKKLANGDIAIIDFTGIVDNKKLASASAQNYELTIGSNSFIKGFETGLIAMKVNQKKTLALTFPSDYHVKELQSKPVTFEVVLKAIKKLEFTPMDETNFKSFLPEQFQSFTSLKAFKSYFHKLMENKKQETILQENNQKIRQFLLTNTKLPFLPEALIKLEANRLLKLQQSQAEQYKIPFEKLLSASNITLTELQDRNIKEAKENVTFALVMKKIADIEKIKVDNNKIKAEIENVIAVEYPFASDEMKKQLFFNMEQQKEFVESIIINRLTTAKIVSYSTH", "aa_hexdigest": "98336f09690dd941cd625078f28adcfb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47480", "query_cov": 1.0, "subject_cov": 0.9166666666666666, "identity": 0.998, "score": 761.0, "evalue": 1.34e-276, "valid": true, "gene": "tig", "product": "trigger factor", "ec_ids": [ "5.2.1.8" ], "uniref50_id": "UniRef50_P47480", "cog_id": "COG0544", "cog_category": "O", "go_ids": [ "GO:0003755", "GO:0005737", "GO:0005854", "GO:0015031", "GO:0043022", "GO:0043335", "GO:0044183", "GO:0051082", "GO:0051083", "GO:0051301", "GO:0061077", "GO:0140839", "GO:0140840" ] }, "pscc": { "uniref50_id": "UniRef50_P47480", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47480" ], "product": "Trigger factor" }, "genes": [ "tig" ], "id": "JPCNHPFPBM_534", "locus": "GCA000292505_00508" }, { "type": "cds", "sequence": "contig_1", "start": 283114, "stop": 284091, "strand": "+", "frame": 1, "gene": null, "product": "LON peptidase substrate-binding domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F21836E" ], "nt": "ATGCCCGTTACGAAGAAAAGTCAGATCTTAGTAGTTAGAGGTCAAGTCATTTTTCCTTTTGTTCCCTTTAGTTTGGATGTTGGCAGGCCCCGTTCGCGTAAGATCATCAAAGCGCTTAAAACTCTGAAAACCAAACGTTTGGTTTTAGTAACCCAAAAGTTTACTGGTGAACAAAACCCTGAGTTTAATGACATCTATCATGTCGGTACACTCTGTGAGATTGATGAGATAGTTGATGTTCCAGGGGTTGATAGTAAAACAGTAGACTACCGTATTAAAGGCAGAGGTTTACAACGGGTTTTAATTGAAAAATTCTCAGATGCAGATATTAATGAAGTTAGTTACCAATTACTTAACTCCACAGTTAAAGATGAAGCTAATGTTGACAGGTTCTTACAGCGAATCTTTCCTGAAAAAGAAGAAATTGAACAGTTAATGGAAGGAGCTGAGAAGTTTTTAGAACTTGAAAACATCAGCAAAACAGTTAATGTTCCTAAGGGTTTAAAGCAACTTGATATTATCACCTTTAAACTGGCTAATCTTGTCCCTAACACTGAAAGTATTAAACAAGCTATCTTAGAGGAAAATGAGATAGCAAACCGATTGGAAAAGATTATCCAAGCAGGGATTGAAGATTTACAGAAGATCCAAGATTATGGTAGATCTAAAAACAAGGAAACTGAGTTTGATAAACTTGACAGTAAAATTACCCGCAAAATTAACGAACAACTCTCAAGACAACAACGTGATTTCTATCTTCGTGAAAAGCTAAGAATTATCCGTGAAGAGATAGGGATTAGTTCCAAAAAAGAGGATGAAGTTGCTAGTATTAGAAAGAAACTGGATGAAAACCCTTACCCTGAAGCCATTAAAAAACGGATTTTAAGTGAACTTGAACACTATGAAAACTCTTCCTCCTCTTCCCAAGAATCAACCTTAACCAAAACTTACATTGATACGCTTTTAAACCTGCCTTGA", "aa": "MPVTKKSQILVVRGQVIFPFVPFSLDVGRPRSRKIIKALKTLKTKRLVLVTQKFTGEQNPEFNDIYHVGTLCEIDEIVDVPGVDSKTVDYRIKGRGLQRVLIEKFSDADINEVSYQLLNSTVKDEANVDRFLQRIFPEKEEIEQLMEGAEKFLELENISKTVNVPKGLKQLDIITFKLANLVPNTESIKQAILEENEIANRLEKIIQAGIEDLQKIQDYGRSKNKETEFDKLDSKITRKINEQLSRQQRDFYLREKLRIIREEIGISSKKEDEVASIRKKLDENPYPEAIKKRILSELEHYENSSSSSQESTLTKTYIDTLLNLP", "aa_hexdigest": "055cd860e4b9ddb0b1d959506f0a4f0f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F21836E", "query_cov": 0.803076923076923, "subject_cov": 1.0, "identity": 0.594, "score": 303.0, "evalue": 1.14e-99, "valid": false, "product": "LON peptidase substrate-binding domain-containing protein", "uniref50_id": "UniRef50_UPI001F21836E" }, "pscc": { "uniref50_id": "UniRef50_UPI001F21836E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F21836E" ], "product": "LON peptidase substrate-binding domain-containing protein" }, "genes": [], "id": "JPCNHPFPBM_535", "locus": "GCA000292505_00509" }, { "type": "cds", "sequence": "contig_1", "start": 284236, "stop": 285501, "strand": "+", "frame": 1, "gene": "lon", "product": "endopeptidase La", "db_xrefs": [ "COG:COG0466", "COG:O", "EC:3.4.21.53", "GO:0004176", "GO:0004252", "GO:0005524", "GO:0005737", "GO:0006508", "GO:0006515", "GO:0016887", "GO:0034605", "GO:0043565", "SO:0001217", "UniRef:UniRef50_P47481", "UniRef:UniRef90_P47481" ], "nt": "ATGTGTTTAGTAGGTCCTCCTGGGGTTGGTAAATCAAGTCTAGCTAAGTCTATTGCAGAAGCATTAGATAAGAAGTTTGTCAAGATCTCATTAGGGGGAGTACATGATGAATCGGAAATCAGAGGTCACCGTAAAACTTACTTAGGTTCTATGCCAGGAAGGATTTTGAAAGGGATGACCCGTGCTAAGGTAATTAATCCCCTCTTTTTACTTGATGAAATTGATAAGATGACCTCCTCCAACCAAGGTTATCCTTCAGGTGCTTTACTTGAAGTATTAGATCCAGAGTTAAATAATAAGTTTAGTGATAACTATGTTGAAGAAGATTATGATCTTTCTAAAGTAATGTTTATCGCTACTGCAAACTACATAGAAGATATCCCTGAAGCTTTACTTGATAGGATGGAGATAATTGAACTCACTTCCTATACAGAACAAGAGAAGATTGAGATAGCAAAAAACCACTTAATTAAGCGTTGCCTTGAGGATGCTGATCTTAACAGTGAAGAATTGAAGTTCACTGATGAAGCAATCAGCTACATCATTAAGTTTTACACAAGAGAAGCGGGGGTTAGACAATTAGAACGATTAATCCAACAAGTTGTAAGAAAGTACATAGTAGCAATGCAAAAAGATGGCATCAAACAAGAAACGATTGATGTAAACGCTGTTAAAAAATACCTTAAGAAGGAGATCTTTGATCACACTATGCGTGATGAAGTGTCTCTACCTGGAATTGTCAACGGGATGGCATACACCCCAACTGGAGGGGACTTACTTCCCATAGAAGTTACCCATGTTGCTGGTAAAGGAGAGTTGATCTTAACTGGTAATTTAAAGCAAACAATGCGAGAAAGCGCTAATGTTGCTTTAGGCTATGTAAAAGCTAATGCAGAGCGTTTTAACATTAATCCTAGTTTGTTTAAAAAGATTGATATTAACATCCATGTTCCAGGTGGGGGAATTCCTAAGGATGGACCTAGTGCTGGTGCTGCTTTGGTAACTGCAATCATCTCATCATTAACTGGTAAGAAAGTAGATCCTACAGTGGCTATGACAGGAGAGATCACTTTAAGAGGCAAAGTGTTGGTTATTGGTGGGGTGAAAGAAAAAACTATCTCAGCTTACCGCGGTGGGGTTACAACTATCTTTATGCCTGAGAAAAACGAGCGCTATTTAGATGAAGTACCCAAAGAGATAGTAGATAAACTTAACATTATCTTTGTTAAGGAATACAGTGATATCTACAACAAGCTTTTCAGTTAG", "aa": "MCLVGPPGVGKSSLAKSIAEALDKKFVKISLGGVHDESEIRGHRKTYLGSMPGRILKGMTRAKVINPLFLLDEIDKMTSSNQGYPSGALLEVLDPELNNKFSDNYVEEDYDLSKVMFIATANYIEDIPEALLDRMEIIELTSYTEQEKIEIAKNHLIKRCLEDADLNSEELKFTDEAISYIIKFYTREAGVRQLERLIQQVVRKYIVAMQKDGIKQETIDVNAVKKYLKKEIFDHTMRDEVSLPGIVNGMAYTPTGGDLLPIEVTHVAGKGELILTGNLKQTMRESANVALGYVKANAERFNINPSLFKKIDINIHVPGGGIPKDGPSAGAALVTAIISSLTGKKVDPTVAMTGEITLRGKVLVIGGVKEKTISAYRGGVTTIFMPEKNERYLDEVPKEIVDKLNIIFVKEYSDIYNKLFS", "aa_hexdigest": "1a44bb6b2e598a29421683a386fb6ae8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0025901479", "query_cov": 0.9857482185273159, "subject_cov": 0.8169291338582677, "identity": 0.537, "score": 468.0, "evalue": 1.55e-159, "valid": false, "gene": "lon", "product": "endopeptidase La", "ec_ids": [ "3.4.21.53" ], "uniref50_id": "UniRef50_A0A7C6Y0Z4", "go_ids": [ "GO:0004252", "GO:0005524", "GO:0006508", "GO:0016887" ] }, "genes": [ "lon" ], "truncated": "5-prime", "pseudogene": { "start": 283936, "stop": 285501, "observations": { "mutation": [ 284089, 284092 ], "stop": [ 284089, 284092 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47481", "query_cov": 1.0, "subject_cov": 0.529559748427673, "identity": 1.0, "score": 819.0, "evalue": 1.17e-293, "gene_start": 1, "gene_end": 421, "reference_start": 375, "reference_end": 795, "reference_sequence": "MPVTKKSQILVVRGQVIFPFVPFSLDVGRPRSRKIIKALKTLKTKRLVLVTQKFTGEQNPEFNDIYHVGTLCEIDEIVDVPGVDSKTVDYRIKGRGLQRVLIEKFSDADINEVSYQLLNSTVKDEANVDRFLQRIFPEKEEIEQLMEGAEKFLELENISKTVNVPKGLKQLDIITFKLANLVPNTESIKQAILEENEIANRLEKIIQAGIEDLQKIQDYGRSKNKETEFDKLDSKITRKINEQLSRQQRDFYLREKLRIIREEIGISSKKEDEVASIRKKLDENPYPEAIKKRILSELEHYENSSSSSQESTLTKTYIDTLLNLPWWQKSKDNSDVKNLIKTLDKNHTGLDKVKERIVEYLAVQLRTQKNKGPIMCLVGPPGVGKSSLAKSIAEALDKKFVKISLGGVHDESEIRGHRKTYLGSMPGRILKGMTRAKVINPLFLLDEIDKMTSSNQGYPSGALLEVLDPELNNKFSDNYVEEDYDLSKVMFIATANYIEDIPEALLDRMEIIELTSYTEQEKIEIAKNHLIKRCLEDADLNSEELKFTDEAISYIIKFYTREAGVRQLERLIQQVVRKYIVAMQKDGIKQETIDVNAVKKYLKKEIFDHTMRDEVSLPGIVNGMAYTPTGGDLLPIEVTHVAGKGELILTGNLKQTMRESANVALGYVKANAERFNINPSLFKKIDINIHVPGGGIPKDGPSAGAALVTAIISSLTGKKVDPTVAMTGEITLRGKVLVIGGVKEKTISAYRGGVTTIFMPEKNERYLDEVPKEIVDKLNIIFVKEYSDIYNKLFS" }, "paralog": false, "identity": 0.9961612284069098, "score": 1009.0, "evalue": 0.0, "description": "Internal stop codon at 284089, 284092. Nonsense mutation around 284089, 284092.", "psc": { "uniref90_id": "UniRef90_P47481", "gene": "lon", "product": "endopeptidase La", "ec_ids": [ "3.4.21.53" ], "uniref50_id": "UniRef50_P47481", "cog_id": "COG0466", "cog_category": "O", "go_ids": [ "GO:0004176", "GO:0004252", "GO:0005524", "GO:0005737", "GO:0006508", "GO:0006515", "GO:0016887", "GO:0034605", "GO:0043565" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47481", "EC:3.4.21.53", "UniRef:UniRef50_P47481", "COG:COG0466", "COG:O", "GO:0004176", "GO:0004252", "GO:0005524", "GO:0005737", "GO:0006508", "GO:0006515", "GO:0016887", "GO:0034605", "GO:0043565" ] }, "pscc": { "uniref50_id": "UniRef50_P47481", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47481" ], "product": "Lon protease" } }, "id": "JPCNHPFPBM_536", "locus": "GCA000292505_00510" }, { "type": "cds", "sequence": "contig_1", "start": 285498, "stop": 285716, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATTAATGCCATCAAAAACCACACCATCCCACCAAAACAAGTTAGCAAGCTAGATATGATTGTTTATTTAGCAGATAAACTAGAACCTAACCGTAAACAGGAACAGTGGAGTGGGGGAATTGAGATTGATCAACTAGTAAAACTAGCTAAAAGCAACTTAAAACAGGCCTATTTAATTACCTTAAAATATGTCCAAAACCTAGTTAAAGACTAA", "aa": "MIINAIKNHTIPPKQVSKLDMIVYLADKLEPNRKQEQWSGGIEIDQLVKLAKSNLKQAYLITLKYVQNLVKD", "aa_hexdigest": "ecff59f73e4a94e84c6848c30caa51b5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8290.6983, "isoelectric_point": 9.477255439758302 }, "id": "JPCNHPFPBM_537", "locus": "GCA000292505_00511" }, { "type": "cds", "sequence": "contig_1", "start": 285780, "stop": 286181, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTGCACGTTTGTTTGTTATGAACGAAAACCTTATCCTTTTAATAAAAAAATAGCAAACCAATTTAATGTTAAATACTTGGCTAAATGCCCACTTGAAATCGCTTCAAGTAAACTTTTAAATCAACCTAGAAAAAAACTGATACCACTGGCAGTTTTAAATTACATCAATACCAATCACCTCTATCTAATTCCCACTTTAAAAGCAATGGTTGATGATAAGAGGTTTCAACACTGTTTAAGGGTTGGTAAACTAGCAAAACAACTAGCAATTGCAAACAAACTAGATGCTAAAAGAGCATTTGTTGCTGGGGCTTATCATGACTTGGCCAAACAACTACCAGTTGATCAGTTAGTTAACATTGCAACTAGTGAACTGAAGATAACTAACTACCCAAGTTGA", "aa": "MCTFVCYERKPYPFNKKIANQFNVKYLAKCPLEIASSKLLNQPRKKLIPLAVLNYINTNHLYLIPTLKAMVDDKRFQHCLRVGKLAKQLAIANKLDAKRAFVAGAYHDLAKQLPVDQLVNIATSELKITNYPS", "aa_hexdigest": "68f963d331e9133c1eeced9e4304f3cb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15161.832799999982, "isoelectric_point": 9.77219829559326 }, "id": "JPCNHPFPBM_538", "locus": "GCA000292505_00512" }, { "type": "cds", "sequence": "contig_1", "start": 286206, "stop": 286550, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01467.32" ], "nt": "TTGAAGCAAAAAATTATTATCTTTGGTGGTTCTTTTGACCCTATTCACAATGCCCATCTCTACATTGCTAAACATGCAATTAAAAAAATAAAAGCGCAAAAGCTTTTTTTTGTACCAACTTACAATGGTATTTTTAAAAATAATTTTCATGCTAGTAATAAGGATAGGATTGCTATGTTAAAGCTAGCAATTAAATCAGTCAACAATGCTTTAGTTTCCAATTTTGATATTAAAACTAAAAACGCTTTTTCAATTAATACTGTTAACCACTTTAAAAGTTGTTATCCAACTAGTGAAATCTATTTTTTAATAGGGAGTGATAAATTAAATGAACTTGAGAAGTGA", "aa": "MKQKIIIFGGSFDPIHNAHLYIAKHAIKKIKAQKLFFVPTYNGIFKNNFHASNKDRIAMLKLAIKSVNNALVSNFDIKTKNAFSINTVNHFKSCYPTSEIYFLIGSDKLNELEK", "aa_hexdigest": "b29232cddfd57f3f64c02283d46472cf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01467.32", "name": "CTP_transf_like", "length": 106, "aa_cov": 0.9298245614035088, "hmm_cov": 0.8867924528301887, "evalue": 1.2165901134990307e-16, "score": 56.22679138183594, "start": 7, "stop": 112 } ], "seq_stats": { "molecular_weight": 13020.078299999983, "isoelectric_point": 9.834990501403805 }, "id": "JPCNHPFPBM_539", "locus": "GCA000292505_00513" }, { "type": "cds", "sequence": "contig_1", "start": 286603, "stop": 287061, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCCAAAAGGAGATGGAGATCTATAACCTCTTTACTTTTCAAATTGATCTTGATAAAAAACTGTTGTTTGAAAAATCAAATGATCAGAAGAATTATTCCAAGATTAGAACCCATTATTTCAAGCATAAATTCAAAAATAAAAGTGCTGTTTTTCTCAATAAAAACCTGATTAAAAATAGCTTAAATAAGGTTCTTTTAAACTTTTCTGATTTTGTTAGTGGTGCTGGAATTGATACAGTTTTTAACCAAATCATTGATGAAGATCCTGAGGTTTTAAACTATTTAAAGCAAGTTAAAAAAGATCTTTCCAAAGAAAACAACGCTACATCCCAACTAACCTTTAATGTAACTATTAACCCTAAAAACACCCTCGCCAATTTTTTTGAAGGATTTAATATTTATCTCCATTTTAATGAAGAAAACAATACAGTGATAGGTAGTTTCTCACTACAATGA", "aa": "MIQKEMEIYNLFTFQIDLDKKLLFEKSNDQKNYSKIRTHYFKHKFKNKSAVFLNKNLIKNSLNKVLLNFSDFVSGAGIDTVFNQIIDEDPEVLNYLKQVKKDLSKENNATSQLTFNVTINPKNTLANFFEGFNIYLHFNEENNTVIGSFSLQ", "aa_hexdigest": "e5692d975c48de59b7a506c11193554a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17789.02409999999, "isoelectric_point": 8.669853401184085 }, "id": "JPCNHPFPBM_540", "locus": "GCA000292505_00514" }, { "type": "cds", "sequence": "contig_1", "start": 287143, "stop": 287247, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATGAAATTAGTTTTATGCAGATCATTAATTGTTTTTCCAAAACAAAAATTAATAAACATGGTGAAATTGTTTTAAAAAGTTGTGCTTTTAAACAAAAATGA", "aa": "MHEISFMQIINCFSKTKINKHGEIVLKSCAFKQK", "aa_hexdigest": "3159e2c441e4613814c0f5d0ff1c4a2d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3982.7601000000013, "isoelectric_point": 9.512648582458496 }, "id": "JPCNHPFPBM_541", "locus": "GCA000292505_00515" }, { "type": "cds", "sequence": "contig_1", "start": 287467, "stop": 287952, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGCTCTTTTTAAACGATTGGTACAACGAAAATCAAGCAATAACAACTGCTGACATTGAAAATGTTAATGAGGTTGAAGACATAGGAAAATTAGTTGAAAAATACAGCACTAATCAACCCCAAAAGTTATCGTTAAATTCAACAGTTTATGTATTACAAACAAAACAAAAACAGTTTTTTTTAAAAAATGATTTCTTTTTTAATAATAACGAAGCAAAGCTGTTTTTTCTAATAACTATGAAGCCCAATGTTTTTGGTCTTGATGATACAGCAATAGCAAATAACTTAAACCTTAAAAAAATTAGTGATTTTTTTAAAGAGATTGATTTTAATGATGAGGATATTCTAAATGACTTTAAACAAGAACAGGAAAAGTTATTGGTTAGAAGAACATTTAACCAACTGTTATTTATGAACAAAAATACTGAAATTCTCAGTGTTGTCAATGACAAGCAAAAGTCAGTAATCCATAACATTGTTTGA", "aa": "MLLFLNDWYNENQAITTADIENVNEVEDIGKLVEKYSTNQPQKLSLNSTVYVLQTKQKQFFLKNDFFFNNNEAKLFFLITMKPNVFGLDDTAIANNLNLKKISDFFKEIDFNDEDILNDFKQEQEKLLVRRTFNQLLFMNKNTEILSVVNDKQKSVIHNIV", "aa_hexdigest": "bd400d7f6bad3266f503ba3a15230cd8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18969.3497, "isoelectric_point": 4.9406953811645495 }, "id": "JPCNHPFPBM_542", "locus": "GCA000292505_00516" }, { "type": "cds", "sequence": "contig_1", "start": 287977, "stop": 288465, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAAAGCCTTTGATTATTCCAAAGCCTTTGAAAAAAACCGAACGAGTGATCCTTCCTTATTGCGATCTAATTTAACTGTTATCAACCGCTTAAGATACCTCAGTGAATACTTTCAAAATGCCTCACTTAAGTATGATCTACTCTACACTAAGGCCAAACAATATATGCAAATTGATAAATTTATCAACGACATGATTCGCAAGGTAAACCATGAGGATGAAATCTTTGGCAAATTTAAAGAAAGAATTTATTTAAGTTTGGGAATTATTAGTGCGGTAGTGTTTGGCATAGTTGAATTTTTTAACTGTGTATGGACTATCTTAACTGTTAGTCAAGAAGTGGTTGATAAAAGTGTTTTGGATCCAAGGAATATTATCTTTATTAGTATAGGTACTATTTTGGTTTTATTTCTTTTGGTTACTATCTTGGTTTTTATGACAAGAAGACTTTATCTGTTTGAAATTAATAAAAAGCATAAAAATTAG", "aa": "MLKAFDYSKAFEKNRTSDPSLLRSNLTVINRLRYLSEYFQNASLKYDLLYTKAKQYMQIDKFINDMIRKVNHEDEIFGKFKERIYLSLGIISAVVFGIVEFFNCVWTILTVSQEVVDKSVLDPRNIIFISIGTILVLFLLVTILVFMTRRLYLFEINKKHKN", "aa_hexdigest": "5d8f68a7283e5c2c9c91b68ce463d716", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19152.35400000002, "isoelectric_point": 9.492792320251464 }, "id": "JPCNHPFPBM_543", "locus": "GCA000292505_00517" }, { "type": "cds", "sequence": "contig_1", "start": 288471, "stop": 289151, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTCAATAAGTTAAACCTAAGTCATTGTATCTCTTTTTACATCTCAGAAGTTTCAGAAGTTTTCTTTGAAAGTATCAACCAACACCCTAGTCGTGATTTTGTTAATAACATTCTTCAAAAGATTAAAACTACTTTAAGTGAAGAAGAGCTTGAAAAGTTAAACAGTATTGAAGAAGTTACTAAGGATGAGAAGATTGTGATCATGCTCAACCATGTTTTGAAAAAGATTGTTTCTAAAACTGGTAGTTCTAAGTGTGACTTATTTAATGTAATCAAACAAGATAGGTTTAATTCTCCAGTGTATATCCAAAGTATTAATGCTTTTGAAAACAACCTTATTAACAATGAATTTGCTGAAAGAAGGTATGACTATTTGATTGAAGTTAATAAAAATTCATACCTTAAAAAATTTGTCAATTCGATCAGAATTTCCTTCTTCTTAGATTTAAGAGCACAAATTTTGTCAGGTTCATTTACCCTTAATTTAGTTAATAAGTCAATTGAAAAACAAAAAAAGACAGAAATTTTTAAAGATATTTTTGTTAATGCCTTAGTTAAGCATTTTATCTGTAATCAACTTTATCCTATCTCTTTAAATTCCTTTATTTTTGACAGTGAAAATCCTAGCAATAAACTCGCTCTAAAAGAACGGATTAAGCTTTTAAAAACAAACTGA", "aa": "MKFNKLNLSHCISFYISEVSEVFFESINQHPSRDFVNNILQKIKTTLSEEELEKLNSIEEVTKDEKIVIMLNHVLKKIVSKTGSSKCDLFNVIKQDRFNSPVYIQSINAFENNLINNEFAERRYDYLIEVNKNSYLKKFVNSIRISFFLDLRAQILSGSFTLNLVNKSIEKQKKTEIFKDIFVNALVKHFICNQLYPISLNSFIFDSENPSNKLALKERIKLLKTN", "aa_hexdigest": "6e0486ba57b45cb497b553a42890351b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26446.24570000004, "isoelectric_point": 9.084514045715334 }, "id": "JPCNHPFPBM_544", "locus": "GCA000292505_00518" }, { "type": "cds", "sequence": "contig_1", "start": 289230, "stop": 289448, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTATGCAGTGATTAACACTTATTTAATCATGTTAATCTCTGTTGAAGAGTTAAGGGTTTACTTTACAAGTAAAGAACCAGCACTGATTTTAAAGGTATTAGATAAAAAAAACACACTAAGAGAAGATCCTGATCAAAACCCTGAAACTGATTTGTATGAACTAATACAGTTTATAGAACAAAACTACTTAAAAAAAGATAAAAAAACCAGCTGA", "aa": "MFYAVINTYLIMLISVEELRVYFTSKEPALILKVLDKKNTLREDPDQNPETDLYELIQFIEQNYLKKDKKTS", "aa_hexdigest": "4ad88746209a892188341895700ea933", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8602.875899999995, "isoelectric_point": 5.017541694641112 }, "id": "JPCNHPFPBM_545", "locus": "GCA000292505_00519" }, { "type": "cds", "sequence": "contig_1", "start": 289554, "stop": 289670, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAATTGATAATGATAATTTTGTCTCTACAAAACAAGTATTTAGAAACCAATTAGAATTACAACTTTTGCATGGGATTGTAATTAATCCTGAGAAGTATGGAATTGGCATGTGA", "aa": "MEIDNDNFVSTKQVFRNQLELQLLHGIVINPEKYGIGM", "aa_hexdigest": "253ffa8719db5ada7c9320d8eb02a9e2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4405.0166, "isoelectric_point": 4.895849418640137 }, "id": "JPCNHPFPBM_546", "locus": "GCA000292505_00520" }, { "type": "cds", "sequence": "contig_1", "start": 289719, "stop": 289883, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCAATCCCAAAAGTGGTAATGATTTTTATCAGTTTGAAAAGGACATAGATGAAAGCATATGTCAAATTAACAAAAAATATCTAACTTTTATTAGCAGTGATAGTAATACCTTTTTAATAGTTAAAAATGATGATGTAAAAGTTATTTCTAACTATGTATGA", "aa": "MLNPKSGNDFYQFEKDIDESICQINKKYLTFISSDSNTFLIVKNDDVKVISNYV", "aa_hexdigest": "d29a41e46fa67643fa85e62367fc1e55", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6283.009700000002, "isoelectric_point": 4.663718986511231 }, "id": "JPCNHPFPBM_547", "locus": "GCA000292505_00521" }, { "type": "cds", "sequence": "contig_1", "start": 290052, "stop": 290345, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGAAAATAGATAGTAAAAGTAATCTTAAAACCTCGATTGAAGAGATGGTAAATAGGTTTAAACATGAGCAGCGAATTAATAAAAAGGACAATGAAAGGGTGTTGATGATTTTTGCTTATATTGCTGCTTTTGTAGTAGGATTTATCAATTTTTTCTCAATGGTCTTTACTATTCTCACTGTAAGTGATCTAAATGCTGGGCTTACTGTACCTAACATCATTGTAATCAGTATCGCTAGTGTTTTAGCTTTTATTTTGATTGTGATTGCTGTTTTATTTCGTTTTAAATGA", "aa": "MQKIDSKSNLKTSIEEMVNRFKHEQRINKKDNERVLMIFAYIAAFVVGFINFFSMVFTILTVSDLNAGLTVPNIIVISIASVLAFILIVIAVLFRFK", "aa_hexdigest": "80a06bf5565933fbf5a52316216e565c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11056.121499999985, "isoelectric_point": 9.773100852966305 }, "id": "JPCNHPFPBM_548", "locus": "GCA000292505_00522" }, { "type": "cds", "sequence": "contig_1", "start": 290675, "stop": 291046, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MG243", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47485", "UniRef:UniRef90_P47485" ], "nt": "ATGATCCATGAGTTGTTTTTATGGATTTTGCTTTCAGTTTTGTTTTTAGTGATTGCTAGTTATTTTTTTATTAACCAAAATAGCAGCGAAGTGCTTAATTTTTTCACTAATTTAGATAAGCCTAATGCAATAGCGATAACAATTAGAACCATAACAATTTTGATTAGTTTTTTACAACTGATTTTTATTGGTTATTTTTGTTTTAGTTCTGAAAAACAAGCTTTACAAGAGATACTGTCAAACACCTTTATGGTTCAAGAAAAACACACTTTAAAATCAAAACCAACTAGCTTAAAAACCAACAATCAACCTGATCCAGCTAATTTACCAGGCGTAATAGCTCTTGATGAAGTGGAAAAATTCATTAATTAA", "aa": "MIHELFLWILLSVLFLVIASYFFINQNSSEVLNFFTNLDKPNAIAITIRTITILISFLQLIFIGYFCFSSEKQALQEILSNTFMVQEKHTLKSKPTSLKTNNQPDPANLPGVIALDEVEKFIN", "aa_hexdigest": "386b9ec8a220134cf0b2eaf9183fe284", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 290375, "stop": 291046, "observations": { "mutation": [ 290447, 290567 ], "stop": [ 290447, 290567 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47485", "query_cov": 1.0, "subject_cov": 0.5442477876106194, "identity": 0.9840000000000001, "score": 225.0, "evalue": 5.4e-73, "gene_start": 1, "gene_end": 123, "reference_start": 104, "reference_end": 226, "reference_sequence": "MQIKVINETNKTVQIFQCAKVKHRALAWLCDVFLLAIVLVVIFLITQAFSDNRFLLFLVLSCSQTILWTVYFIFLPFFWDGKTLFRNLLKIKLFAFDKRFLRIMIHELFLWILLSVLFLVIASYFFINQNSSEALNFFTNLDKPNAIAITIRTITILISFLQLIFIGYFCFSSEKQALQEILSNTFMVQEKHTLKSKPTSLKTNNQPDPANLPGVIALDEVEKLIN" }, "paralog": false, "identity": 0.9820627802690582, "score": 426.0, "evalue": 1.79e-154, "description": "Internal stop codon at 290447, 290567. Nonsense mutation around 290447, 290567.", "psc": { "uniref90_id": "UniRef90_P47485", "product": "Uncharacterized protein MG243", "uniref50_id": "UniRef50_P47485", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47485", "UniRef:UniRef50_P47485", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47485", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47485" ], "product": "Uncharacterized protein MG243" } }, "id": "JPCNHPFPBM_549", "locus": "GCA000292505_00523" }, { "type": "cds", "sequence": "contig_1", "start": 291052, "stop": 291816, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12", "PFAM:PF13245.12", "PFAM:PF13604.12", "PFAM:PF00580.27" ], "nt": "ATGAATGAACAACAAAAACAAGCAATTAGTTGTGGAAAAGGGGTTAATGTTGTTTATTCTGGAGCAGGTACTGGTAAAACAACAATTATTACTAATCGCTTTGCATACTTGGTTAATAAAGAAAAAGTTGATCCTAGCAGAATTTTAGCAATCACCTTTACTAAGAAAGCTGCTAAGGAGATGCAGTTTAGAATCTTGAAACTAATAGATAGTTCTTTAGCTGAGAAAACAAATATCTATACATTTCACAGCTTTTGCAATAAGTTTTTAATTCAAACATTAAAAAAGCGCTTTATCATCGATGATGATATTAGCTATTTCCTAAAGGAATTTTTAGCTGATTCAAAACTCGATATCAACCTAGCGAAACAAATTATTGATAACTTTAAAAATACTTTTGCTGATTTTGAAATAAATAAGTTGGATCAAGATGAAAGGTTAATTAGTTTATGTGAGCATTCACTTCTAAATAAAGATGAAGAATATTCCACTTTAAAAACCCAACTGATTAATGCATTCATTAGCTATGAAAAGAATAAGATATTAAACAATAAACTTGATTTTCATGATCTTTTAATTAAAACTTGTAATTTATTGAGTAATGATAATGATTTACTTAATCAGTGGAGTGAACAGTTTCAGCATATTTTAGTTGATGAATTTCAAGATACCAACCAAATCCAATATGAACTGATCAAGATGTTAGTAACTAAAAATAAAAACTTGTTTTTGGTAGGTGATAATAACCAGATGATTTACCGCTGA", "aa": "MNEQQKQAISCGKGVNVVYSGAGTGKTTIITNRFAYLVNKEKVDPSRILAITFTKKAAKEMQFRILKLIDSSLAEKTNIYTFHSFCNKFLIQTLKKRFIIDDDISYFLKEFLADSKLDINLAKQIIDNFKNTFADFEINKLDQDERLISLCEHSLLNKDEEYSTLKTQLINAFISYEKNKILNNKLDFHDLLIKTCNLLSNDNDLLNQWSEQFQHILVDEFQDTNQIQYELIKMLVTKNKNLFLVGDNNQMIYR", "aa_hexdigest": "734c02e5eed2df0bf894bfc136c2e196", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 263, "aa_cov": 0.9803149606299213, "hmm_cov": 0.9391634980988594, "evalue": 3.3658907693717414e-08, "score": 28.653270721435547, "start": 2, "stop": 250 }, { "id": "PF13245.12", "name": "AAA_19", "length": 253, "aa_cov": 0.9803149606299213, "hmm_cov": 0.5177865612648221, "evalue": 5.104620353416507e-27, "score": 89.79872131347656, "start": 5, "stop": 253 }, { "id": "PF13604.12", "name": "AAA_30", "length": 65, "aa_cov": 0.24015748031496062, "hmm_cov": 0.9384615384615385, "evalue": 1.0044695219033605e-09, "score": 33.5244255065918, "start": 2, "stop": 62 }, { "id": "PF00580.27", "name": "UvrD-helicase", "length": 263, "aa_cov": 1.0, "hmm_cov": 0.9657794676806084, "evalue": 3.7586843095300954e-77, "score": 254.6881561279297, "start": 1, "stop": 254 } ], "seq_stats": { "molecular_weight": 29636.69570000004, "isoelectric_point": 6.902265739440918 }, "id": "JPCNHPFPBM_550", "locus": "GCA000292505_00524" }, { "type": "cds", "sequence": "contig_1", "start": 291955, "stop": 292314, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13361.12" ], "nt": "ATGGCCTATGAAAAACAAGTTAAAACTGAAAAAAATCTCTTGTTTTCAACTTTAAATTCTGATAAAAAACCTGTTTATTTTCAAGCTGAATCAGTTGAAAATCAAGCCAATTGGATCTTCAATAAAATCAAAGCACTAAACCAAACAGAAAAGATTAATTTTAAGGATATGGCCATCTTGTTTAGAAAGAACAGAGATATTACTACTATGGTTGAATTGATTGAAGCGGATGGAACAATTCCCTTACCTAAACAAAAGAGTTATTTTAACCAACTAGTAAAACTCCAGCGGGTTTTAATTGCGATTTCAACCAGAACAAATCTTGATATTAAAAGAGCTTTGCAAGCCCTAAAAATTTGA", "aa": "MAYEKQVKTEKNLLFSTLNSDKKPVYFQAESVENQANWIFNKIKALNQTEKINFKDMAILFRKNRDITTMVELIEADGTIPLPKQKSYFNQLVKLQRVLIAISTRTNLDIKRALQALKI", "aa_hexdigest": "427d93c4722c36a82a5ec96882383682", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13361.12", "name": "UvrD_C", "length": 72, "aa_cov": 0.6050420168067226, "hmm_cov": 0.9722222222222222, "evalue": 2.4313674864772233e-08, "score": 28.814903259277344, "start": 10, "stop": 81 } ], "seq_stats": { "molecular_weight": 13862.067299999977, "isoelectric_point": 9.800693321228025 }, "id": "JPCNHPFPBM_551", "locus": "GCA000292505_00525" }, { "type": "cds", "sequence": "contig_1", "start": 292606, "stop": 293163, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13361.12", "PFAM:PF13538.12" ], "nt": "TTGGAATTTGACTCAATTATTGAAAACAGCAGTGATACAATCAATTTGCTAACCGTTCATGCAGCAAAAGGACTTGAGTTTGAAGCTGTATTTATTTATGGCATGAATCAAGGGGATTTTCCCTTATTTTTAAGTCAAAATCAAAATGACGAACAACATTTAATTGATGAATTAAAACTGTTTTATGTTGCTATCACAAGAGCAAAACGTTTTTTGTTTATCACTGCGGTTTTACAAATAAATAACAATTCTATAAAACCATCTAGTTTTTTAAATTACATCAATAAAAGTGAGTATTTAGACATTGCTACTATTAACTATGTATTAGAGCAGGATGATGATTTTTTTGATTCAACTAAAAAAACAGACTATACAAAGAAACTAAGAAAAGAAAGTTTAGACATTATAGTGGGTGATTTAGTTACTAGTAGATACTTTGGAAAAGGAGTTGTAGTTGAAGTGAGAGACAAAGAGGTTTTAGTAGCTTTTAAAGACACACGCTATGGGATGAAATGGATCTTAAAAAACCATAAATCACTAACAAAAGCTTTATATTAA", "aa": "MEFDSIIENSSDTINLLTVHAAKGLEFEAVFIYGMNQGDFPLFLSQNQNDEQHLIDELKLFYVAITRAKRFLFITAVLQINNNSIKPSSFLNYINKSEYLDIATINYVLEQDDDFFDSTKKTDYTKKLRKESLDIIVGDLVTSRYFGKGVVVEVRDKEVLVAFKDTRYGMKWILKNHKSLTKALY", "aa_hexdigest": "08120c03d7ba61bab0a9b782373cf333", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13361.12", "name": "UvrD_C", "length": 68, "aa_cov": 0.3675675675675676, "hmm_cov": 0.9705882352941176, "evalue": 9.014603837604544e-25, "score": 82.85357666015625, "start": 10, "stop": 77 }, { "id": "PF13538.12", "name": "UvrD_C_2", "length": 59, "aa_cov": 0.31891891891891894, "hmm_cov": 0.8135593220338984, "evalue": 2.537386997558498e-11, "score": 38.41843032836914, "start": 16, "stop": 74 } ], "seq_stats": { "molecular_weight": 21431.244100000004, "isoelectric_point": 5.657378196716309 }, "id": "JPCNHPFPBM_552", "locus": "GCA000292505_00526" }, { "type": "cds", "sequence": "contig_1", "start": 293166, "stop": 293663, "strand": "+", "frame": 3, "gene": "fAU1", "product": "5-formyltetrahydrofolate cyclo-ligase", "db_xrefs": [ "COG:COG0212", "COG:H", "EC:6.3.3.2", "GO:0005524", "GO:0005737", "GO:0006730", "GO:0009396", "GO:0030272", "GO:0035999", "GO:0046872", "RefSeq:WP_009885782.1", "SO:0001217", "UniParc:UPI00001394EB", "UniRef:UniRef100_P47487", "UniRef:UniRef50_P75430", "UniRef:UniRef90_P47487" ], "nt": "ATGGTTGATAAAAACAGTTTAAGAAAATTAATGCTTCTAAAAAGAGCAGAACTAAATGATCTTGAAAAATCGCATTTAGATCAAAAGATTAACCAAAAATTAATGGCTTTTTTAATAACAAGACCAACAATTAAAAATTTAGCACTTTACATTCCCATTAAAAACGAAGTGGCTTTTTTAGATAACTTTCTAGATTTTCTTAAGTTAAATAAAATTACAAGCTGTTTTCCTAGTATTGTTGATCAATTTAACATGAAGTTTATTGATCAAAATAATAATGAAATTAACCCTAATGATATTGATTGTTTTTTTATCCCTTTATTAGCTTTTAATAAGGCAAACCACAGGATTGGTTTTGGTAAGGGTTATTATGACCGTTATTTATCATTAACTAGCAAAAAACAACTAAAAATAGGGATAGCATATGACTTTCAATATGCAGAATTCACTAATGATCCTTGGGATTATCAATTAGATTTAATTATTTGCAATGGATAA", "aa": "MVDKNSLRKLMLLKRAELNDLEKSHLDQKINQKLMAFLITRPTIKNLALYIPIKNEVAFLDNFLDFLKLNKITSCFPSIVDQFNMKFIDQNNNEINPNDIDCFFIPLLAFNKANHRIGFGKGYYDRYLSLTSKKQLKIGIAYDFQYAEFTNDPWDYQLDLIICNG", "aa_hexdigest": "a9380ce962d3874d46c708a41fac1d43", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394EB", "ncbi_nrp_id": "WP_009885782.1", "uniref100_id": "UniRef100_P47487", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394EB", "RefSeq:WP_009885782.1", "UniRef:UniRef100_P47487" ] }, "ips": { "uniref100_id": "UniRef100_P47487", "uniref90_id": "UniRef90_P47487" }, "psc": { "uniref90_id": "UniRef90_P47487", "gene": "FAU1", "product": "5-formyltetrahydrofolate cyclo-ligase", "ec_ids": [ "6.3.3.2" ], "uniref50_id": "UniRef50_P75430", "cog_id": "COG0212", "cog_category": "H", "go_ids": [ "GO:0005524", "GO:0005737", "GO:0006730", "GO:0009396", "GO:0030272", "GO:0035999", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P75430", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75430" ], "product": "5-formyltetrahydrofolate cyclo-ligase" }, "genes": [ "fAU1" ], "id": "JPCNHPFPBM_553", "locus": "GCA000292505_00527" }, { "type": "cds", "sequence": "contig_1", "start": 293656, "stop": 293811, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13277.11" ], "nt": "ATGGATAACATAAAGGTTCTTTTTTTAGGTGATGTTTATGGCAAAGCTGGTAGAAAGATTATTAGTGATCATCTTCCCATAATTAAAAAAAAGTATCAGTTAAATCTAATTATTGCAAATGCTGAAAACACTACTAATGGTAAGGGTTTAAGTTGA", "aa": "MDNIKVLFLGDVYGKAGRKIISDHLPIIKKKYQLNLIIANAENTTNGKGLS", "aa_hexdigest": "fe570e65d19f9feb85cecc0e6cdc0fc2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13277.11", "name": "YmdB", "length": 44, "aa_cov": 0.8627450980392157, "hmm_cov": 1.0, "evalue": 3.714494178200541e-15, "score": 50.94171142578125, "start": 7, "stop": 50 } ], "seq_stats": { "molecular_weight": 5627.5596, "isoelectric_point": 9.698381996154787 }, "id": "JPCNHPFPBM_554", "locus": "GCA000292505_00528" }, { "type": "cds", "sequence": "contig_1", "start": 293857, "stop": 294501, "strand": "+", "frame": 1, "gene": null, "product": "Putative metallophosphoesterase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PBN9" ], "nt": "ATGGGTAACCATACCTGGTTTCAAAAGCAAGATTTAGAACTTGTTTTAAACCAAGTTGATGTTATTCGCCCACTTAACTTAATGCAAGATTTTAACTATTTTCAGCTTGGCAAAGGGAGTTATTTATTTAGCTTAAATGGTTTGAAAATAAGGATTACTAACTTGTTAGGAACAAGTATTAACTTACCATTTGCAATAACAAACCCATTTGTGGAATTAAAAAAGTTAGTTTTAACTAAAGATTGTGATCTTCATATCGTTGATTTTCATGCTGAAACAACTAGCGAAAAAAATGCTTTTTGCATGGTTTTTGATGGTTATGTTACTGCTATCTTAGGAACCCACACCCATGTTCCTAGTAATGATTTAAGAATCACTCCTAAAGGAAGTGTTTACATTACTGATGTAGGGATGTGTGGTCCTGGATTTGGTAGTGTTATTGGTGCTAATCCCAAGCAATCAATTAAGTTATTTTGTACAGGTGAAAGACAGTTTTTTGAGGTTTCTAATTGTGGTGCACAACTCAATGGCGTCTTCTTTGAAGTTTGTTCAAAAACCAATCAAGTTGTGAAAATAGAACAGATTAGAATAGTTTTAGATGATGAAAAGTATTTAGCTAATGACTACTTTAATTTAGTTGAGTAA", "aa": "MGNHTWFQKQDLELVLNQVDVIRPLNLMQDFNYFQLGKGSYLFSLNGLKIRITNLLGTSINLPFAITNPFVELKKLVLTKDCDLHIVDFHAETTSEKNAFCMVFDGYVTAILGTHTHVPSNDLRITPKGSVYITDVGMCGPGFGSVIGANPKQSIKLFCTGERQFFEVSNCGAQLNGVFFEVCSKTNQVVKIEQIRIVLDDEKYLANDYFNLVE", "aa_hexdigest": "5ad3d6f535a230a8c00420df0697ca96", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PBN9", "query_cov": 0.8738317757009346, "subject_cov": 0.9540816326530612, "identity": 0.54, "score": 195.0, "evalue": 6.88e-60, "valid": false, "product": "Putative metallophosphoesterase", "ec_ids": [ "3.1.4.16" ], "uniref50_id": "UniRef50_A0A3B0PBN9", "kegg_orthology_id": "K09769" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PBN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PBN9" ], "product": "Putative metallophosphoesterase" }, "genes": [], "id": "JPCNHPFPBM_555", "locus": "GCA000292505_00529" }, { "type": "cds", "sequence": "contig_1", "start": 294684, "stop": 294869, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTACAGCTAACACAAAGAATGTAATAAGACTTGCTAAAGAAACATACTTAGTTATTAAAGTAATCATTATCCAAATTAAAAGACAGATTAAAAATCATCATAAAGATATTGCAAGTAAAGATCCACCAGTAGTAGCAATTGCCTTACCACCCTTAAACTTAAAATACAGCGGAAAGATATGA", "aa": "MITANTKNVIRLAKETYLVIKVIIIQIKRQIKNHHKDIASKDPPVVAIALPPLNLKYSGKI", "aa_hexdigest": "0db4a42fbae561feb4819429db8ec9a4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6872.260500000002, "isoelectric_point": 10.237402153015136 }, "id": "JPCNHPFPBM_556", "locus": "GCA000292505_00530" }, { "type": "cds", "sequence": "contig_1", "start": 294968, "stop": 295204, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCAAGCTAGTGCAATTGCCATTTTGGTCATTTTTAGCCTAGTTTCTGGTTATCTGTTAGGTTCAATTATTTTTGCTGATATTTTCAGCAAAATACTCAAGAAAAACGTCAGGGAATTTGGTTCAAAAAACCCAGGAGCTACTAACTCAATGCGTGTTTTTGGCTTAAAAATTGGTTTTTTGGTGGCTATTTTTGATGCATTTAAAGGTTTTTTTGCTTTTTTATTAACCTGA", "aa": "MNQASAIAILVIFSLVSGYLLGSIIFADIFSKILKKNVREFGSKNPGATNSMRVFGLKIGFLVAIFDAFKGFFAFLLT", "aa_hexdigest": "6d9ab0306fbff042aa1955b2303479c3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8497.064099999996, "isoelectric_point": 10.118586921691893 }, "id": "JPCNHPFPBM_557", "locus": "GCA000292505_00531" }, { "type": "cds", "sequence": "contig_1", "start": 295191, "stop": 295847, "strand": "-", "frame": 2, "gene": "trmK", "product": "Putative tRNA methyltransferase MG248", "db_xrefs": [ "COG:COG2384", "COG:J", "EC:2.1.1.-", "GO:0005737", "GO:0008033", "GO:0032259", "GO:0160105", "KEGG:K06967", "RefSeq:WP_009885786.1", "SO:0001217", "UniParc:UPI00001394FC", "UniRef:UniRef100_P47490", "UniRef:UniRef50_P47490", "UniRef:UniRef90_P47490" ], "nt": "ATGAAAAAAAGGATTAGCACAATTGCTAATTTAGTTCAATCATTTAATCCTAAGCTAGTGTATGACATCGGTTGTGATCATTCCTATCTAACATCCTATCTAATAAAAACTAATCAAAACCTAACCATTGTTAACAGTGACATTAGTAAAAATGCTTTATTAAGTAATTACCAAAAATTTAAAAATAACAATAATATCCACTTCTTTGTTAGTGATGGATTTAATAACCTTCCAGAACTAAATATTAATCCCAAAATAGGGGTAATTGCTGGACTTGGTGGATTGAAAATCATTAACATAATTAGCCAAAAAGAAAACTTTATTAATCGTTTTGTAATCCAACCCCAATCTAATTTAATTGAATTACGTAGTTTTTTAAGTTTAAACAGTTGGGATATTGTTAATGAAACACTAGTTCAGGATCGAGAGTTTATATATCCTATTTTGGTTATTGAAAAACTAAAAAAACCGTTTAAATTAACAAAGGAATTGGTTATTTTAGGACCAAAGCTAATTAACTTTAAAGATAAACACTGTTTAATGAAACACTACCAATGCCTATTGAGAGTTTATCAACCCAAACAAAAACCTAGCTTGATGGATCTTAAGATAATTGAAACATTAAATAAGATAATTACAAGCTATGAATCAAGCTAG", "aa": "MKKRISTIANLVQSFNPKLVYDIGCDHSYLTSYLIKTNQNLTIVNSDISKNALLSNYQKFKNNNNIHFFVSDGFNNLPELNINPKIGVIAGLGGLKIINIISQKENFINRFVIQPQSNLIELRSFLSLNSWDIVNETLVQDREFIYPILVIEKLKKPFKLTKELVILGPKLINFKDKHCLMKHYQCLLRVYQPKQKPSLMDLKIIETLNKIITSYESS", "aa_hexdigest": "3e7f95a754f8ca28e1394692b172531c", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001394FC", "ncbi_nrp_id": "WP_009885786.1", "uniref100_id": "UniRef100_P47490", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001394FC", "RefSeq:WP_009885786.1", "UniRef:UniRef100_P47490" ] }, "ips": { "uniref100_id": "UniRef100_P47490", "uniref90_id": "UniRef90_P47490" }, "psc": { "uniref90_id": "UniRef90_P47490", "gene": "TrmK", "product": "Putative tRNA methyltransferase MG248", "ec_ids": [ "2.1.1.-" ], "uniref50_id": "UniRef50_P47490", "cog_id": "COG2384", "cog_category": "J", "kegg_orthology_id": "K06967", "go_ids": [ "GO:0005737", "GO:0160105", "GO:0032259", "GO:0008033" ] }, "pscc": { "uniref50_id": "UniRef50_P47490", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47490" ], "product": "Putative tRNA methyltransferase MG248" }, "genes": [ "trmK" ], "id": "JPCNHPFPBM_558", "locus": "GCA000292505_00532" }, { "type": "cds", "sequence": "contig_1", "start": 295831, "stop": 296346, "strand": "-", "frame": 1, "gene": null, "product": "RNA polymerase sigma factor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EFD7AF2", "UniRef:UniRef90_UPI001EFD7AF2" ], "nt": "ATGGTAGAAACCATTAACCGCTTATCTAAAGCAGAACGGGCTTTGTATCAAGAGTTAGGGCGAGAACCTACTGATGAGGAGTTAGCTGAAAAGATGGGAGGACAAGCTGAAGGATTTAATGTTAAAAAGATTGCTGAAATTAAACGGTTAAGTTTAGATCCAGTTTCGCTTGATAAAACAGTTGGACATGATGAAGAGTCCCAGTTTGGTGATTTTGTTAAAGACACAGACGCTCAAACTCCTGACGAGTTTACCGAAAGCCGTTCAAATTCAGAAAAAATTGATGAATTGTTGAACAATAATCTTTCTGAACAAGAAGAGTTAATTGTTAGAATGCGGATTGGCATGCCCCCTTACAATGAACCTAAAACACTTGATGAAGTAGGTCAAAAGATTTTGATCCCTAGAGAGAAGATCAGACAAATTGAAAACAAAGCAATTAGAAAATTGAGACATGCAGTTAGAAACAATCCTATTAGTATGTCATTTCTAAGAATTAATGAAAAAAAGGATTAG", "aa": "MVETINRLSKAERALYQELGREPTDEELAEKMGGQAEGFNVKKIAEIKRLSLDPVSLDKTVGHDEESQFGDFVKDTDAQTPDEFTESRSNSEKIDELLNNNLSEQEELIVRMRIGMPPYNEPKTLDEVGQKILIPREKIRQIENKAIRKLRHAVRNNPISMSFLRINEKKD", "aa_hexdigest": "1631d541be8aa6fb9ebe83d89529a93e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 295831, "stop": 296646, "observations": { "mutation": [ 296415, 296451 ], "stop": [ 296415, 296451 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001EFD7AF2", "query_cov": 1.0, "subject_cov": 0.5245398773006135, "identity": 0.895, "score": 280.0, "evalue": 2.54e-92, "gene_start": 1, "gene_end": 171, "reference_start": 156, "reference_end": 326, "reference_sequence": "MEEHIHEFRANQDLSIIDEDIEELTTKNISNRDKVDDNVRFFLGSLDSSKMLDFESEQRIAKVLNSTDEESRKYAINQLVTSNLRLVVSIAKKHLERGLDFNDLIQEGNLGLLKAISKFNWSLGNKFSTYATWWIKQAITRAIADQARTVRIPVHMVETINRLAKAERALNQELGREPTAEELAEKMGGQAEGFTVKKIAEIKRLSLDPVSLDKTVGHDEESQFGDFVRDTDAQMPDEFTESRSNYEKIDELLNNCLSEQEELIVRMRIGMPPYNETKTLDEVSQKIKIPREKIRQIETKAIRKLRQAVRNNHMSLSFMRGNEKKD" }, "paralog": false, "identity": 0.9261992619926199, "score": 477.0, "evalue": 1.69e-172, "description": "Internal stop codon at 296415, 296451. Nonsense mutation around 296415, 296451.", "psc": { "uniref90_id": "UniRef90_UPI001EFD7AF2", "product": "RNA polymerase sigma factor", "uniref50_id": "UniRef50_UPI001EFD7AF2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001EFD7AF2", "UniRef:UniRef50_UPI001EFD7AF2" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001EFD7AF2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EFD7AF2" ], "product": "RNA polymerase sigma factor" } }, "id": "JPCNHPFPBM_559", "locus": "GCA000292505_00533" }, { "type": "cds", "sequence": "contig_1", "start": 296449, "stop": 296853, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04542.20" ], "nt": "TTGGATGAACTACGTGATAAGGGGATTCAACTCCAACACGATGTTGAAGAGCACATCCATGAATTTCGTGCTAACCAAGACCTTTCAATTATTGATGAAGATATTGAAGAGTTAACAAGTAAGAACATCTCTAACCGTGATAAGGTTGATGATAATGTTAGGTTCTTTTTAGGATCACTTGACTTTTCTAAAATGTTAGATTTTGAATCTGAACAGCGGATTGCCAAGGTTTTAAATAGTACTGATGAAGAGTCACGTAAGTATGCAATTAATCAGTTGGTTACTTCAAACTTAAGACTAGTTGTTTCTATTGCCAAAAAACACCTAGAAAGAGGGTTGGATTTTAATGATTTAATTCAAGAGGGTAATTTGGGGCTTTTAAAAGCTATTTCCAAATTTAACTGA", "aa": "MDELRDKGIQLQHDVEEHIHEFRANQDLSIIDEDIEELTSKNISNRDKVDDNVRFFLGSLDFSKMLDFESEQRIAKVLNSTDEESRKYAINQLVTSNLRLVVSIAKKHLERGLDFNDLIQEGNLGLLKAISKFN", "aa_hexdigest": "0980eb09e85c9b0dd63841bd4f602a37", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04542.20", "name": "Sigma70_r2", "length": 42, "aa_cov": 0.31343283582089554, "hmm_cov": 1.0, "evalue": 7.921035201510265e-13, "score": 43.18305969238281, "start": 93, "stop": 134 } ], "seq_stats": { "molecular_weight": 15517.207499999988, "isoelectric_point": 4.886641502380371 }, "id": "JPCNHPFPBM_560", "locus": "GCA000292505_00534" }, { "type": "cds", "sequence": "contig_1", "start": 296959, "stop": 297324, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCCACTGACAAAAAAACGCTAGGCGAAAAACCCAATTCAACCAAACCAGAACTATCTGAAGAATTAATTGCTGAACTTAAAAAACAGCGTATTCTTGAAAAGAATCGTCCTTACAAAAAGATGATTTATGTTGACAATAAAGTGCAACGCAAACACCGTCATGAAAACATCGCTTTTCTCAAAACCCTTCATGAAAATAAGGAGAGTGATGTTCCTAAAAAAAGAAGGGGTAGAAAACCTAAACACGCTCCTTTAAAAGAAAAAAATAATCTGAAGTTATTTGATATCTTAGAAGGATCGTTAAAAAGCCACATTGAAAATGATGACACCAACACAGTCATCAACCTTCTAACAGAAGCTTGA", "aa": "MSTDKKTLGEKPNSTKPELSEELIAELKKQRILEKNRPYKKMIYVDNKVQRKHRHENIAFLKTLHENKESDVPKKRRGRKPKHAPLKEKNNLKLFDILEGSLKSHIENDDTNTVINLLTEA", "aa_hexdigest": "c31079dcb7bfcc478a226fe210da7c25", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14179.188199999977, "isoelectric_point": 9.724169349670408 }, "id": "JPCNHPFPBM_561", "locus": "GCA000292505_00535" }, { "type": "cds", "sequence": "contig_1", "start": 297369, "stop": 297503, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAATTAAAGATATTTTTTTAGATGAATTAACTTGTTATCAAGCTAATGATTTTCAAAACTATCTAAAGACCTTTCAAACGTTATTAAAACAACAAAAGCAGCGCTTAAAAAATTTAAAGTTAACGCTATAA", "aa": "MEIKDIFLDELTCYQANDFQNYLKTFQTLLKQQKQRLKNLKLTL", "aa_hexdigest": "dccd2730aebfe8c31a4b258b9260da37", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5380.241800000001, "isoelectric_point": 8.889303779602052 }, "id": "JPCNHPFPBM_562", "locus": "GCA000292505_00536" }, { "type": "cds", "sequence": "contig_1", "start": 297561, "stop": 297686, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTGAATTAAAACAATCCCATCTAGATGAAGTTTTTAAGGAATGTAACTTTAATTTGTTTGATGTTGAACTTTTTATTGAAAAAGCAAGGATCTATTGGAGTGAAAATCAAACTGCTAACTGA", "aa": "MVELKQSHLDEVFKECNFNLFDVELFIEKARIYWSENQTAN", "aa_hexdigest": "9a52b9846b11b0a8189fffd7a06df2f6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4979.554900000001, "isoelectric_point": 4.591988182067872 }, "id": "JPCNHPFPBM_563", "locus": "GCA000292505_00537" }, { "type": "cds", "sequence": "contig_1", "start": 297729, "stop": 297929, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTAAACTACTTGAATATAGTGATGAAAAAACTCTGTATGAAACAGTTTTAAAACACAAAGAAAAACTTGTATCTAAGTTTGATAACAACCGTTTTTACATAAATACTTCAGGCCATGCTCAACCACCACAAGAATTGCAAAAAACCACTGCAGCACTAGTGCAAACAGCTTTTGAAGAAGCAGTTAATGAGTTGTGA", "aa": "MVKLLEYSDEKTLYETVLKHKEKLVSKFDNNRFYINTSGHAQPPQELQKTTAALVQTAFEEAVNEL", "aa_hexdigest": "6c3fd154940092a323ffeee1993b7cce", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7628.5366, "isoelectric_point": 5.585192680358887 }, "id": "JPCNHPFPBM_564", "locus": "GCA000292505_00538" }, { "type": "cds", "sequence": "contig_1", "start": 298152, "stop": 298781, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08275.17", "PFAM:PF13362.12", "PFAM:PF13662.12" ], "nt": "ATGAATTATTTAGTTGAAAAAAGAAAGCTTAATAAAACATTAATTGAACAGTTTCAGCTAGGACTTGCTTTTCACAATGAAGATAAGTATCTATGTGAAAGTATGGAAAGATACCCCTTCATTAATCCAAAGATAAAACCGAGTGAATTGTATCTTTTTTCAAAAACTAACCAGCAAGGTCTTGGCTTTTTTGACTTTAATACCAAAAAAGCTACCTTTCAAAACCAGATTATGATCCCTATCCATGACTTTAATGGTAACCCGGTTGGTTTTTCAGCAAGAAGTGTTGATAACATCAACAAACTGAAATATAAAAATAGTGCTGATCACGAATTTTTTAAAAAAGGGGAGCTGTTATTTAACTTTCACAGGTTAAATAAAAACCTCAATCAACTCTTTATTGTGGAAGGTTATTTTGATGTTTTTACACTAACAAACTCCAAGTTTGAAGCTGTTGCATTAATGGGATTAGCATTAAATGATGTGCAAATTAAAGCCATTAAAGCTCACTTTAAGGAGTTACAAACCTTAGTTTTAGCACTTGATAATGATGCTAGTGGCCAAAATGCTGTGTTTAGCTTAATTGAAAAACTTAATAACAACAATTTTATTGTGGAAATTGTTCAGTGA", "aa": "MNYLVEKRKLNKTLIEQFQLGLAFHNEDKYLCESMERYPFINPKIKPSELYLFSKTNQQGLGFFDFNTKKATFQNQIMIPIHDFNGNPVGFSARSVDNINKLKYKNSADHEFFKKGELLFNFHRLNKNLNQLFIVEGYFDVFTLTNSKFEAVALMGLALNDVQIKAIKAHFKELQTLVLALDNDASGQNAVFSLIEKLNNNNFIVEIVQ", "aa_hexdigest": "4a2f6bb18690df8547ab732aa2c055b9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08275.17", "name": "DNAG_N", "length": 123, "aa_cov": 0.5885167464114832, "hmm_cov": 0.9024390243902439, "evalue": 1.0365348413733742e-22, "score": 75.69379425048828, "start": 2, "stop": 124 }, { "id": "PF13362.12", "name": "Toprim_3", "length": 81, "aa_cov": 0.3684210526315789, "hmm_cov": 0.9382716049382716, "evalue": 3.2182349404231716e-08, "score": 29.07499885559082, "start": 132, "stop": 208 }, { "id": "PF13662.12", "name": "Toprim_4", "length": 81, "aa_cov": 0.36363636363636365, "hmm_cov": 1.0, "evalue": 4.0693109271610616e-13, "score": 44.54798126220703, "start": 131, "stop": 206 } ], "seq_stats": { "molecular_weight": 24185.49400000001, "isoelectric_point": 8.503782844543458 }, "id": "JPCNHPFPBM_565", "locus": "GCA000292505_00539" }, { "type": "cds", "sequence": "contig_1", "start": 298950, "stop": 299192, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01807.25" ], "nt": "ATGGTCAATAAAAGCAACAGTTTAGATGAACTTTTAAAGCAGATTAAAATTACTGAAATTATTCAACACTACGGGGTTAAAATCCAAACTAAGGGTAATAGTCTACTTGCTTTATGTCCTTTTCATGATGATAAAAATCCTTCTATGTCCATTTCCAGTTCTAAAAACATCTTTAAGTGTTGGGCTTGTAATGCAGCTGGCAACGGAATAGCGTTTATCCAAAAGCATGACCAGTTAGATTGA", "aa": "MVNKSNSLDELLKQIKITEIIQHYGVKIQTKGNSLLALCPFHDDKNPSMSISSSKNIFKCWACNAAGNGIAFIQKHDQLD", "aa_hexdigest": "7ae53ff976d465a91306833323e38d78", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01807.25", "name": "Zn_ribbon_DnaG", "length": 76, "aa_cov": 0.95, "hmm_cov": 0.9868421052631579, "evalue": 5.82752265688644e-27, "score": 88.54666137695312, "start": 5, "stop": 80 } ], "seq_stats": { "molecular_weight": 8904.173199999994, "isoelectric_point": 8.50629711151123 }, "id": "JPCNHPFPBM_566", "locus": "GCA000292505_00540" }, { "type": "cds", "sequence": "contig_1", "start": 299203, "stop": 299376, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25" ], "nt": "GTGTGCTTTGAGACAACTGGTAGTATTGGTAAAAGGTATCGAAAAGCAGATGCAATTGGAATAAAGTATGCAGTCACTTTTGACTTTGAAAGTTTAGAAGATAATGCAGTTACCATCAGAGATAGAGATACTTTAGTTCAACAGCGAATTGCTATCAAAGAATTACCACAATGA", "aa": "MCFETTGSIGKRYRKADAIGIKYAVTFDFESLEDNAVTIRDRDTLVQQRIAIKELPQ", "aa_hexdigest": "1f878184f893ddd4ec01356c38fbc426", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_F2R163", "query_cov": 0.9649122807017544, "subject_cov": 0.8333333333333334, "identity": 0.509, "score": 68.2, "evalue": 5.19e-14, "valid": false, "product": "Glycyl-tRNA synthetase", "uniref50_id": "UniRef50_A0A8T5UJC6" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 52, "aa_cov": 0.9122807017543859, "hmm_cov": 1.0, "evalue": 4.980410334777154e-16, "score": 53.81874465942383, "start": 5, "stop": 56 } ], "seq_stats": { "molecular_weight": 6523.367, "isoelectric_point": 6.138861274719238 }, "id": "JPCNHPFPBM_567", "locus": "GCA000292505_00541" }, { "type": "cds", "sequence": "contig_1", "start": 299380, "stop": 299688, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTAACAGGGGTGATTTTGATCTAAAACAACACCAAGAGTTTTCAAAAAAGAGCATGAGTTTTTTTGATAGCGAATTAAACCAACATTTCTTACCTTTCATAATCGAACCTGCGGTTGGCATTGAACGGTTATTTTATGCACTAATTGTCAGTAGTTATAGGAGAGAAATTATTAATGAGGAAGAACGGGAAGTATTGAGTTTACCATTTGACTTATGTCCTGAACAAATTATTGTTTTACCACTTGTAAATAAACTTAAAAAAGAAGCATTTTCTGTATTTGAAACGCTAGCAAAAACAAGGTGA", "aa": "MANRGDFDLKQHQEFSKKSMSFFDSELNQHFLPFIIEPAVGIERLFYALIVSSYRREIINEEEREVLSLPFDLCPEQIIVLPLVNKLKKEAFSVFETLAKTR", "aa_hexdigest": "563bb330efc747edeb11aa1c89f3d862", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11980.710599999986, "isoelectric_point": 5.387790870666504 }, "id": "JPCNHPFPBM_568", "locus": "GCA000292505_00542" }, { "type": "cds", "sequence": "contig_1", "start": 299692, "stop": 300129, "strand": "-", "frame": 1, "gene": null, "product": "aminoacyl--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0017CB9A86" ], "nt": "GTGAGGGATTTTAACTTACTTTTTCAAACTGAAATCGGGGTTGTAAACAACGATAAACGCCTTGTTTTTCTCCGTCCTGAGACTGCTCAAGGTAGCTTTATTAACTTTAAAAATATCTTGCAAGCTAAGAAGCGTAATTTACCTTTTGCTATTGCCCAGTTTGGTAAAAGCTTTCGTAATGAAATCACCCCAGGTAACTTCTTGTTTAGAACTAGAGAGTTTGAACAGTTTGAAATTGAGTGGTTTTGTAAACCTGATGATGCAAATTCGCTGTTTGAAAAACAATTAATAATGGTAGAACAGTTTCTACAAACAGTGTTAAAAATTAACCCAGAATTGTTAAAAAAACATGAATATGATCAATCAGAATTGGCTCATTATGCCAAAAAAACTACTGACTTTTTGTTTAATTTTCCCCACGGATTAAAGGAGTTATGA", "aa": "MRDFNLLFQTEIGVVNNDKRLVFLRPETAQGSFINFKNILQAKKRNLPFAIAQFGKSFRNEITPGNFLFRTREFEQFEIEWFCKPDDANSLFEKQLIMVEQFLQTVLKINPELLKKHEYDQSELAHYAKKTTDFLFNFPHGLKEL", "aa_hexdigest": "4c2afbe9d13e754f2886f84a1fdbfdf8", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0017CB9A86", "query_cov": 0.8137931034482758, "subject_cov": 0.8489208633093526, "identity": 0.534, "score": 137.0, "evalue": 5.23e-39, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "uniref50_id": "UniRef50_UPI0017CB9A86" }, "pscc": { "uniref50_id": "UniRef50_UPI0017CB9A86", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0017CB9A86" ], "product": "aminoacyl--tRNA ligase-related protein" }, "genes": [], "id": "JPCNHPFPBM_569", "locus": "GCA000292505_00543" }, { "type": "cds", "sequence": "contig_1", "start": 300295, "stop": 300522, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAAGTTTACAACCAAGAAGTTTATGTTCAGTTTCTCAAACAACATGGTTTTGTATTTCAGAGTAGTGAAATTTACAACGGTTTAAACAATAGTTGGGATTTTGGTCCATTAGGTGCAGTTTTAAAACAACAAATCAAACAAGCTTTATATAACTTTTTTATTAAAAATAAAGCTGATGTTCTTTTAGTTGAAACCCCTATTATTCTCAGCGAATTGGTTTGA", "aa": "MAKVYNQEVYVQFLKQHGFVFQSSEIYNGLNNSWDFGPLGAVLKQQIKQALYNFFIKNKADVLLVETPIILSELV", "aa_hexdigest": "99c1362b2bb61d9d81f7f6d847e552ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8638.8954, "isoelectric_point": 6.534346961975097 }, "id": "JPCNHPFPBM_570", "locus": "GCA000292505_00544" }, { "type": "cds", "sequence": "contig_1", "start": 300761, "stop": 301066, "strand": "-", "frame": 3, "gene": "rlmB", "product": "23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB", "db_xrefs": [ "COG:COG0566", "COG:J", "EC:2.1.1.-", "GO:0003723", "GO:0005737", "GO:0006396", "GO:0008173", "GO:0032259", "SO:0001217", "UniRef:UniRef50_P47494", "UniRef:UniRef90_P47494" ], "nt": "TTGGTAGCAGTTTTAGATACTAACCAACTCTTAATTCCACTTGATCAACTGGTTAAAGTAGTTGAAAATAAGAAGTGCAGTACACTTGTGATGTTAGATGAGATCCAAGACCCTTATAATTTTGGTGCTATACTAAGAACTTGTTTGGCTAGTGAGGTTGATGGAATTATCTTTAAAAAGAATAACCAAGTCCCTATCAACAATACAGTCATGAAAACTAGTATGGGTAGTGTTTTTTATCAAAACTTAGTGCAAGTAGCTAACTTAAGTTATACGATCACTAAGCTAAAAGAAATAGGATTTTGA", "aa": "MVAVLDTNQLLIPLDQLVKVVENKKCSTLVMLDEIQDPYNFGAILRTCLASEVDGIIFKKNNQVPINNTVMKTSMGSVFYQNLVQVANLSYTITKLKEIGF", "aa_hexdigest": "0ab4095d911ecfd522f47747da48ebf0", "start_type": "TTG", "rbs_motif": null, "genes": [ "rlmB" ], "truncated": "both", "pseudogene": { "start": 300542, "stop": 301270, "observations": { "mutation": [ 300553, 300733, 300763, 301105 ], "stop": [ 300553, 300733, 300763, 301105 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47494", "query_cov": 1.0, "subject_cov": 0.41735537190082644, "identity": 0.99, "score": 194.0, "evalue": 7.3e-61, "gene_start": 1, "gene_end": 101, "reference_start": 69, "reference_end": 169, "reference_sequence": "MKKPRQQSCLFGVKAFEEAINNQVHIKLVNISIRHKKLIPLIEAKKINFQIHSTNWFNNQYRDINHQELVAVLDTNQLLIPLDQLVKVVENKKCSTLVMLDEIQDPYNFGAILRTCLASEVDGIIFKKNNQVPINNTVMKTSMGSVFYQNLVQVANLSYTITKLKEIGFWTVVSTLDPIWKPIDYRKVDFAKKILIVGNEDRGVNQLITKNADCRIKIPMNNKINSLNVSVALGIILFAWKN" }, "paralog": false, "identity": 0.9834710743801653, "score": 462.0, "evalue": 6.57e-169, "description": "Internal stop codon at 300553, 300733, 300763, 301105. Nonsense mutation around 300553, 300733, 300763, 301105.", "psc": { "uniref90_id": "UniRef90_P47494", "gene": "rlmB", "product": "23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB", "ec_ids": [ "2.1.1.-" ], "uniref50_id": "UniRef50_P47494", "cog_id": "COG0566", "cog_category": "J", "go_ids": [ "GO:0003723", "GO:0005737", "GO:0006396", "GO:0008173", "GO:0032259" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47494", "EC:2.1.1.-", "UniRef:UniRef50_P47494", "COG:COG0566", "COG:J", "GO:0003723", "GO:0005737", "GO:0006396", "GO:0008173", "GO:0032259" ] }, "pscc": { "uniref50_id": "UniRef50_P47494", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47494" ], "product": "Uncharacterized tRNA/rRNA methyltransferase MG252" } }, "id": "JPCNHPFPBM_571", "locus": "GCA000292505_00545" }, { "type": "cds", "sequence": "contig_1", "start": 301103, "stop": 301270, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACCACGCCAACAAAGTTGCTTATTTGGTGTTAAAGCCTTTGAGGAAGCGATTAATAACCAAGTTCACATTAAATTGGTAAACATTTCCATTCGTCACAAAAAACTAATACCTTTAATTGAAGCTAAAAAAATTAATTTTCAAATCCATTCAACTAACTGA", "aa": "MKKPRQQSCLFGVKAFEEAINNQVHIKLVNISIRHKKLIPLIEAKKINFQIHSTN", "aa_hexdigest": "81f499d59e3fd886272d7cc121b78df0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6395.550499999998, "isoelectric_point": 10.227280616760254 }, "id": "JPCNHPFPBM_572", "locus": "GCA000292505_00546" }, { "type": "cds", "sequence": "contig_1", "start": 301612, "stop": 301872, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01406.26" ], "nt": "ATGGCTTTATATAACCAGCCCATTACCAAACATTGGATGCATATTGGTCATTTGATGATTGAAAACCAAAAGATGTCAAAGTCATTGCAGAACTTCTTGTTAGCAGTTGATTTTCTTAACTTTCATGATTTTCGTGTTTTGCGTTGGATCTTTTACCAAAAACACTATTTGCATCCTATTGATCTAAACCAATCATTGATTGAAAAAGCTAATAATGATATTCAAAGGATTGCAAAAACACTTAATGTTGCTAGAACCTGA", "aa": "MALYNQPITKHWMHIGHLMIENQKMSKSLQNFLLAVDFLNFHDFRVLRWIFYQKHYLHPIDLNQSLIEKANNDIQRIAKTLNVART", "aa_hexdigest": "9fbfdc96d5f93acce165d133e66bd98f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01406.26", "name": "tRNA-synt_1e", "length": 70, "aa_cov": 0.813953488372093, "hmm_cov": 1.0, "evalue": 5.745917128741201e-24, "score": 80.05188751220703, "start": 2, "stop": 71 } ], "seq_stats": { "molecular_weight": 10375.995899999987, "isoelectric_point": 9.695351982116701 }, "id": "JPCNHPFPBM_573", "locus": "GCA000292505_00547" }, { "type": "cds", "sequence": "contig_1", "start": 302044, "stop": 302583, "strand": "-", "frame": 1, "gene": "cysS", "product": "cysteine--tRNA ligase", "db_xrefs": [ "EC:6.1.1.16", "GO:0004817", "GO:0005524", "GO:0005737", "GO:0006423", "SO:0001217", "UniRef:UniRef50_UPI001F010120", "UniRef:UniRef90_UPI001F010120" ], "nt": "ATGAAGCAATTTGAACATAGGTTTATGATTGTTGACAGTGTTAGTCAAAAACCAACAACACTAGTTCAAAAAACCATTAACATTTATCTCTGTGGACCCACAGTTTATAACGATTTGCACTTAGGCAACACCAGACCATTAATTGTTTTTGATGTTTTAAATAGAGTTTTAAAAAAGGCTAAATATACCGTTAATTTTGTTCAAAACATCACTGATATTGACGATAAGATCATCAAGATTGCTCAACAACAAGAAGTAAGCGAATCAGTTGTTACAAAACAACAAATCACTGCTTACAAATCACTTTTAAAAAAACTAAATATTCTGCCTATTAAACATATTCAAATCACTGAAAAAATCGATAAAATCCCTGACTATATTGATCAATTAGTAAATCAAAACCATGCTTATGTTTCAACTCAAAACAACGTTTATTTTGCAGTTAATTCACTAAAGCAATATGGTTATCTAGCTAACCGAATGGTGCATTTAGAAGAAACTGATACTGATAAAAAGAACAAATTGGATTTTGTACTTTGA", "aa": "MKQFEHRFMIVDSVSQKPTTLVQKTINIYLCGPTVYNDLHLGNTRPLIVFDVLNRVLKKAKYTVNFVQNITDIDDKIIKIAQQQEVSESVVTKQQITAYKSLLKKLNILPIKHIQITEKIDKIPDYIDQLVNQNHAYVSTQNNVYFAVNSLKQYGYLANRMVHLEETDTDKKNKLDFVL", "aa_hexdigest": "ebf4ad70eb3ca52b5351bb899bda96ef", "start_type": "ATG", "rbs_motif": null, "genes": [ "cysS" ], "truncated": "5-prime", "pseudogene": { "start": 301754, "stop": 302583, "observations": { "deletion": [ 301803 ], "mutation": [ 301986, 302019, 302046 ], "stop": [ 301764, 301986, 302019, 302046 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F010120", "query_cov": 1.0, "subject_cov": 0.6485507246376812, "identity": 1.0, "score": 350.0, "evalue": 9.99e-121, "gene_start": 1, "gene_end": 179, "reference_start": 1, "reference_end": 179, "reference_sequence": "MKQFEHRFMIVDSVSQKPTTLVQKTINIYLCGPTVYNDLHLGNTRPLIVFDVLNRVLKKAKYTVNFVQNITDIDDKIIKIAQQQEVSESVVTKQQITAYKSLLKKLNILPIKHIQITEKIDKIPDYIDQLVNQNHAYVSTQNNVYFAVNSLKQYGYLANRMVHLEETDTDKKNKLDFVLWKITTAGIKWNSKWGLGRPGWHVECAFLIDYCFKNELTIHGGGVDLKFPHHENENALHMALYNQPITKHWMHIGHLMIENQKDVKVIAELLVSSWFS" }, "paralog": false, "identity": 0.9855072463768116, "score": 540.0, "evalue": 8.19e-198, "description": "Internal stop codon at 301764, 301986, 302019, 302046. Frameshift due to deletion around 301803. Nonsense mutation around 301986, 302019, 302046.", "psc": { "uniref90_id": "UniRef90_UPI001F010120", "gene": "cysS", "product": "cysteine--tRNA ligase", "ec_ids": [ "6.1.1.16" ], "uniref50_id": "UniRef50_UPI001F010120", "go_ids": [ "GO:0004817", "GO:0005524", "GO:0005737", "GO:0006423" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F010120", "EC:6.1.1.16", "UniRef:UniRef50_UPI001F010120", "GO:0004817", "GO:0005524", "GO:0005737", "GO:0006423" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F010120", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F010120" ], "product": "cysteine--tRNA ligase" } }, "id": "JPCNHPFPBM_574", "locus": "GCA000292505_00548" }, { "type": "cds", "sequence": "contig_1", "start": 302570, "stop": 303766, "strand": "-", "frame": 3, "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "db_xrefs": [ "COG:COG0272", "COG:L", "EC:6.5.1.2", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0006310", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47496", "UniRef:UniRef90_P47496" ], "nt": "TTGGAAGCGTTTGACAAAAAACGAGAACAGTTAACTTTTAATCTTGATGGTTTAGTTATTAAACTAAACAGCTTGCTTTTTTATCAACAATTAGGTGCTACAAGTAAATCACCACGTTGGGCAATAGCATTTAAATTTAGTCCTAAATTTGTTCAAACTAAATTAACAGCAGTTCTTATAACGATTGGTAGAACTGGTAGAGTGAACTATACTGCTAAATTAGAAAGTGTTAATTTAGATGGAACAAAAGTAACAGCTGCTACTTTACATAACTTTGATTACATTAAAACTAAAGACATTAGGATCAATGACACTGTTGTTATCTATAAAGCTGGGGAAATTATCCCTAAAGTACTAAAGGTAAACCTTGAAAAAAGAAAAAATGACACTATCATAATTCAAGAGCAAAAATATTGTCCTTCATGTAATTCAAAACTAGTCAAAATAGTTGATGAAGTTGATCAGTATTGTACCAATGAAACTTGTAAGGAGCGAAACATCCAGTTAATTAACTATTTTGTTTCTAAAACTGCTATGGACATTAACGGGTTGAATATTAATACTATTACCAAACTTTATGAACACAATTTGGTTAGATCTATAGTTGATCTTTATGATTTAAAAGACAAGAAAAACCAAGTTTTAAAATTAGATCTGAAGATTGGTGATAAACTTTTCAACAAGTTAGTTGATAACATTGAAAATTCAAAACAAAAAGGAATGGCTAGATTACTAACAGGACTTGGTATTAAGCATGTTGGTAATGTATTAGCTAAGAATTTAGCTAATCATTTTAAAAATATCAAAGCATTACAGCATGCTAGCTTAGAGAACTTAATTAGTTTAAATGATGTAGGAATAACAGTAGCTGAATCATTGTATAACTGGTTTCATGACCCTAACCATTTGCAGTTAATTGAACAACTTGAATTAAGACAAGTAAAAACAGATCAATTACCACTGAAAATTAACTTTGAAACTAACAGTATTTATTTTCAAAAACGCTTTCTTATTACCGGTAGCTTTAACATTAGTCGTGACCAAATTAAGGATTTATTATCAGCTAAGTTTGATTGCCAGTTTGCAAGTGAAGTCAAACCAACAGTTGACTTTGTTATTGCAGGAAACAAACCAACTTTAAGAAAAATCAATCACGCCAAAGAACTGAACATTCCTATCATTAATGAAGCAATTTGA", "aa": "MEAFDKKREQLTFNLDGLVIKLNSLLFYQQLGATSKSPRWAIAFKFSPKFVQTKLTAVLITIGRTGRVNYTAKLESVNLDGTKVTAATLHNFDYIKTKDIRINDTVVIYKAGEIIPKVLKVNLEKRKNDTIIIQEQKYCPSCNSKLVKIVDEVDQYCTNETCKERNIQLINYFVSKTAMDINGLNINTITKLYEHNLVRSIVDLYDLKDKKNQVLKLDLKIGDKLFNKLVDNIENSKQKGMARLLTGLGIKHVGNVLAKNLANHFKNIKALQHASLENLISLNDVGITVAESLYNWFHDPNHLQLIEQLELRQVKTDQLPLKINFETNSIYFQKRFLITGSFNISRDQIKDLLSAKFDCQFASEVKPTVDFVIAGNKPTLRKINHAKELNIPIINEAI", "aa_hexdigest": "3810e779aa5b76cdb2d5b6862c0b2d4b", "start_type": "TTG", "rbs_motif": null, "genes": [ "ligA" ], "truncated": "both", "pseudogene": { "start": 302564, "stop": 304066, "observations": { "mutation": [ 302572, 303829 ], "stop": [ 302572, 303829 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47496", "query_cov": 1.0, "subject_cov": 0.6039453717754173, "identity": 0.997, "score": 691.0, "evalue": 1.59e-245, "gene_start": 1, "gene_end": 398, "reference_start": 260, "reference_end": 657, "reference_sequence": "MMDVKLKIQQLVNLIKNYDYHYYVLSEPLIDDFEYDMLYKSLQQLEKDHPDLIQIDSPTQRVGGEAVKGFKKLNHNSPMLSLENAFSTKEIANFIDNINFQTNSKNEFVVEPKIDGVSISLTYKNGVLVHALTRGDGSVGEDVLNNVKTIKSIPLTIPFTKTIEIRGEIFVDKKTFLAINNQLEKPFANARNLAAGTIRNLNSEITAQRKLRALFYYIPNGLEESITTQTMVLEQLKQWKFPVSDTIRVFQNKFQLINYLEAFDKKREQLTFNLDGLVIKLNSLLFYQQLGATSKSPRWAIAFKFSPKFVQTKLTAVLITIGRTGRVNYTAKLESVNLDGTKVTAATLHNFDYIKTKDIRINDTVVIYKAGEIIPKVLKVNLEKRKNDTIIIQEQKYCPSCNSKLVKIVDEVDQYCTNETCKERNIQLINYFVSKTAMDINGLNINTITKLYEHNLVRSIVDLYDLKDKKNQVLKLDLKIGDKLFNKLVDNIENSKQKGMARLLTGLGIKHVGNVLAKNLANHFKNIKALQHASLENLISLNDVGITVAESLYNWFHDPNHLQLIEQLELRQVKTDQLPLKINFETNSIYFQKRFLITGSFNISRDQIKDLLSAKFDCQFASEVKPTVDFVIAGNKPTLRKINHAKELNIPIINEAIWT" }, "paralog": false, "identity": 0.996, "score": 886.0, "evalue": 0.0, "description": "Internal stop codon at 302572, 303829. Nonsense mutation around 302572, 303829.", "psc": { "uniref90_id": "UniRef90_P47496", "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "ec_ids": [ "6.5.1.2" ], "uniref50_id": "UniRef50_P47496", "cog_id": "COG0272", "cog_category": "L", "go_ids": [ "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0006310", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47496", "EC:6.5.1.2", "UniRef:UniRef50_P47496", "COG:COG0272", "COG:L", "GO:0003911", "GO:0005829", "GO:0006260", "GO:0006281", "GO:0006288", "GO:0006310", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47496", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47496" ], "product": "DNA ligase" } }, "id": "JPCNHPFPBM_575", "locus": "GCA000292505_00549" }, { "type": "cds", "sequence": "contig_1", "start": 303827, "stop": 304540, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01653.23" ], "nt": "ATGGATGTGAAATTGAAGATTCAACAGCTGGTTAACTTAATAAAAAACTATGACTATCACTACTATGTTTTAAGCGAACCTTTAATTGATGATTTTGAGTATGATATGTTGTATAAGTCACTCCAACAATTAGAAAAAGATCATCCTGATTTAATCCAAATTGATTCCCCTACCCAAAGGGTGGGAGGAGAAGCTGTGAAGGGTTTTAAAAAGTTAAACCATAACAGTCCAATGCTCTCTTTGGAAAATGCTTTTTCAACTAAAGAAATTGCTAATTTTATTGATAATATTAACTTTCAAACAAACTCAAAAAATGAATTTGTAGTTGAACCTAAAATTGATGGAGTTAGTATCTCTCTAACTTATAAAAATGGTGTTTTAGTTCATGCTTTAACCAGAGGAGATGGAAGTGTTGGGGAAGATGTTTTAAATAATGTTAAAACCATTAAATCTATCCCTTTAACAATCCCTTTCACAAAAACAATTGAGATTAGGGGTGAGATTTTTGTTGATAAAAAAACTTTTTTAGCAATTAACAATCAACTTGAAAAACCATTTGCTAATGCAAGGAATCTAGCAGCAGGTACAATACGTAATTTAAACAGTGAAATCACTGCACAGCGCAAATTAAGGGCATTATTTTATTACATCCCTAATGGTTTGGAAGAGTCAATCACTACTCAAACTATGGTTTTAGAACAGCTTAAGCAGTGA", "aa": "MDVKLKIQQLVNLIKNYDYHYYVLSEPLIDDFEYDMLYKSLQQLEKDHPDLIQIDSPTQRVGGEAVKGFKKLNHNSPMLSLENAFSTKEIANFIDNINFQTNSKNEFVVEPKIDGVSISLTYKNGVLVHALTRGDGSVGEDVLNNVKTIKSIPLTIPFTKTIEIRGEIFVDKKTFLAINNQLEKPFANARNLAAGTIRNLNSEITAQRKLRALFYYIPNGLEESITTQTMVLEQLKQ", "aa_hexdigest": "c38251d25be9a4c74c59183158d0b399", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01653.23", "name": "DNA_ligase_aden", "length": 181, "aa_cov": 0.7172995780590717, "hmm_cov": 1.0, "evalue": 1.7649044788906102e-65, "score": 216.0682830810547, "start": 68, "stop": 237 } ], "seq_stats": { "molecular_weight": 26954.529200000026, "isoelectric_point": 6.122093772888182 }, "id": "JPCNHPFPBM_576", "locus": "GCA000292505_00550" }, { "type": "tRNA", "sequence": "contig_1", "start": 304610, "stop": 304683, "strand": "-", "gene": "trnG", "product": "tRNA-Gly(gcc)", "amino_acid": "Gly", "anti_codon": "gcc", "score": 80.2, "nt": "GCAGATATAGTTCAATGGTAGAACATAACCTTGCCAAGGTTAAGATGCGGGTTCGATTCCCGTTATCTGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 304649, 304651 ], "id": "JPCNHPFPBM_32", "locus": "GCA000292505_00551" }, { "type": "cds", "sequence": "contig_1", "start": 304719, "stop": 305012, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATCTGAAAACCAAATTGCAATCCTGGATTATATTTTTAACCAAGTCAACCAGCCCAATCAACCAAAAATAGTTTGGTTCTCTGGGGAAGGGGAGGATGAGAAAATTAATTTTTTAATCCGCTTAAATGATTTTTTTAAACCAAAATTTGTTGAAAATACTAATGATAGTAGCTTTTTATTAAGTTTTAGAAACCATGTTGAAACTAAAAATTCAACGCCTTTAACCCAAGCTAACTTTGCTAATATTGCCAACAAATTACTAGCGGTTTTATTTGGTTCATTGCAATGA", "aa": "MESENQIAILDYIFNQVNQPNQPKIVWFSGEGEDEKINFLIRLNDFFKPKFVENTNDSSFLLSFRNHVETKNSTPLTQANFANIANKLLAVLFGSLQ", "aa_hexdigest": "8442183bc8b656aa3f9e060de47a4d1b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11153.428599999983, "isoelectric_point": 4.967807579040527 }, "id": "JPCNHPFPBM_577", "locus": "GCA000292505_00552" }, { "type": "cds", "sequence": "contig_1", "start": 305142, "stop": 305882, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MG255", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47497", "UniRef:UniRef90_P47497" ], "nt": "TTGAGTTTTATTAAACAACAATGGCCTATTCTAACAACATTGGTAACAGTAGGGACCACATTAGGTACTCCAGTATTTTCATTAACAATAGCCCAACAAGATGGTATTAAGCAAAATGCGGGGAATGATGTCTTTATCTTTTTGATTATCTTCTCTGTTTTTTCTATTAGTTTAGGGCTTGTTTCATCACTTATCTTTTTAGTCTCCAGCTTATTTTCAATTCATCAGAAAAAAACGTTGGATGCTTTGGATAAAGTGTTATCAAAATTTATTGATAAATACTTTTTTTTAGATGAAAAAGAGATCAAAAAACAACTTAAATATCAGTTTAAAAACAACGGTGTTTGCTTTTTTTATGGTTTTGATTTTGATCAGGCAGAGTTTCTTGAACAATCAATGAACTTAATGTTGTTGTTAAAGCAAACCAATTGTTTTATTTTAGTTGGTTGTAAAGAAAGTGAAATGACGCTCATTAAAAACAAGATAGAACCTAACATTAACTTAAAACAAAACAGTTTTTATCTGGATTTAAGCAATGAAATTTCACAAGTAGAACAGATCAGCAAATTTAACTTGTTGTTTAGGCAATTGAGGCTCAGTTCTGAACTATTTTACTTAGAAGATTTTTTTGATTATCTAACTACTGCAAAACAGATAGTTAACTTTCTTTTTAAAACAAAACCTAACCTTGATCAATTTCAAGAAAATAAAAAGAAATTATTTGATTTTTTAGCGTTATGA", "aa": "MSFIKQQWPILTTLVTVGTTLGTPVFSLTIAQQDGIKQNAGNDVFIFLIIFSVFSISLGLVSSLIFLVSSLFSIHQKKTLDALDKVLSKFIDKYFFLDEKEIKKQLKYQFKNNGVCFFYGFDFDQAEFLEQSMNLMLLLKQTNCFILVGCKESEMTLIKNKIEPNINLKQNSFYLDLSNEISQVEQISKFNLLFRQLRLSSELFYLEDFFDYLTTAKQIVNFLFKTKPNLDQFQENKKKLFDFLAL", "aa_hexdigest": "52bf2151dd320a783f0d5a9276fd5c5a", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 304842, "stop": 305816, "observations": { "mutation": [ 305010, 305040, 305088 ], "stop": [ 305010, 305040, 305088 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47497", "query_cov": 0.9105691056910569, "subject_cov": 0.6136986301369863, "identity": 0.991, "score": 419.0, "evalue": 3.81e-145, "gene_start": 1, "gene_end": 224, "reference_start": 142, "reference_end": 365, "reference_sequence": "MESENQIAILDYIFNQVNQPNQPKIVWFSGEGEDEKINFLIRLNDFFKPKFVENTNDSSFLLSFRNHVETKNSTPLTQANFANIANKLLAVLFGSLQWKQLNKPTGNWFLVILFLALLWLRQCWLKLQLTKISKFVNQKGILSFIKQQWPILTTLVTVGTTLGTPVFSLTIAQQDGIKQNAGNDVFIFLIIFSVFSISLGLVSSLIFLVSSLFSIRQKKTLDALDKVLSKFIDKYFFLDEKEIKKQLKYQFKNNGVCFFYGFDFDQAEFLEQSMNLMLLLKQTNCFILVGCKESEMTLIKNKIEPNINLKQNSFYLDLSNEISQVEQISKFNLLFRQLRLSSELFYLEDFFDYLTTAKQIVNFLF" }, "paralog": false, "identity": 0.9876543209876543, "score": 620.0, "evalue": 3.13e-227, "description": "Internal stop codon at 305010, 305040, 305088. Nonsense mutation around 305010, 305040, 305088.", "psc": { "uniref90_id": "UniRef90_P47497", "product": "Uncharacterized protein MG255", "uniref50_id": "UniRef50_P47497", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47497", "UniRef:UniRef50_P47497", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47497", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47497" ], "product": "Uncharacterized protein MG255" } }, "id": "JPCNHPFPBM_578", "locus": "GCA000292505_00553" }, { "type": "tRNA", "sequence": "contig_1", "start": 306334, "stop": 306408, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(cct)", "amino_acid": "Arg", "anti_codon": "cct", "score": 54.4, "nt": "CTTCCCTTGGTGCAATGGACAGCACAACTGAGTCCTAATCAGTAAATAGAGGTTCAACTCCTCTAGGGAAGGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 306338, 306340 ], "id": "JPCNHPFPBM_10", "locus": "GCA000292505_00554" }, { "type": "tRNA", "sequence": "contig_1", "start": 306457, "stop": 306530, "strand": "+", "gene": "trnW", "product": "tRNA-Trp(cca)", "amino_acid": "Trp", "anti_codon": "cca", "score": 75.5, "nt": "GGGGGTGTAGTTTAGTGGTAGAACAACAGTCTCCAAAACTGTCTGTGTGGGTTCGATTCCTTCCACCCCCGCCA", "db_xrefs": [ "SO:0000271" ], "anti_codon_pos": [ 306489, 306491 ], "id": "JPCNHPFPBM_11", "locus": "GCA000292505_00555" }, { "type": "cds", "sequence": "contig_1", "start": 306538, "stop": 306717, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCACTTCAATTCAAACTTTAAAGAACGCTTTAATAAGATTGCAAAAAAAGTTAATTCACTGGACAGTGAATACTATGAGTTTTCTTCTTTTATTGAAAGGATTAGAACTACCTTTGGACTTTTAATTGCTTTAACTGTTTTAAGTAACCTAATCATTATTAGCTTTGTTTTAATTTGA", "aa": "MHFNSNFKERFNKIAKKVNSLDSEYYEFSSFIERIRTTFGLLIALTVLSNLIIISFVLI", "aa_hexdigest": "6e8559266a6db41d3163c11aae0ea9c0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6933.033199999994, "isoelectric_point": 9.300805473327635 }, "id": "JPCNHPFPBM_579", "locus": "GCA000292505_00556" }, { "type": "cds", "sequence": "contig_1", "start": 306919, "stop": 307059, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGATTAAATAAAACTATTAAGAATTTCGCTAGTGATTATGGTTTTAAGATTGTTAATAAATATAACAGTTTAACAAGCGGAATTTTTGTGTTTGACTTTGCTGATTATGTTAGTTTTGAAGCCAACCTGACTAATTGA", "aa": "MRLNKTIKNFASDYGFKIVNKYNSLTSGIFVFDFADYVSFEANLTN", "aa_hexdigest": "9349d36f0229c30105ecd7763246f589", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5304.936200000001, "isoelectric_point": 8.063141441345213 }, "id": "JPCNHPFPBM_580", "locus": "GCA000292505_00557" }, { "type": "cds", "sequence": "contig_1", "start": 307354, "stop": 307647, "strand": "+", "frame": 1, "gene": "rpmE", "product": "50S ribosomal protein L31", "db_xrefs": [ "COG:COG0254", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "RefSeq:WP_014894636.1", "SO:0001217", "UniParc:UPI00027B39B1", "UniRef:UniRef100_UPI00027B39B1", "UniRef:UniRef50_P47499", "UniRef:UniRef90_P47499" ], "nt": "ATGAAAAAGGCAATCCACTTTCAGAGTCAACCAGTTGTTTTTAACTGTGCTTCATGCAATAGCAACTTTACCATTGACTCCACTGCCAAACAAAAGGATCTTGCCATTGACATTTGTGGAAAATGTCATCCTTTTTACATAGGGCAATTAACCAAACAAACCGTGCATGGACGGGCTGAAAAACTTTCTCAAAAGTTCAACGCTGGAAAGGCTTTTTTAGAAAAAAAAACTAAAAAGAGTAACCAAGCTAAAGTTGAAAAACAAACTAGGCACCGTTCTATTAACGAGCTTTAG", "aa": "MKKAIHFQSQPVVFNCASCNSNFTIDSTAKQKDLAIDICGKCHPFYIGQLTKQTVHGRAEKLSQKFNAGKAFLEKKTKKSNQAKVEKQTRHRSINEL", "aa_hexdigest": "ddff84a5b259b6e38d69a5bc62418b04", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B39B1", "ncbi_nrp_id": "WP_014894636.1", "uniref100_id": "UniRef100_UPI00027B39B1", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B39B1", "RefSeq:WP_014894636.1", "UniRef:UniRef100_UPI00027B39B1" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B39B1", "uniref90_id": "UniRef90_P47499" }, "psc": { "uniref90_id": "UniRef90_P47499", "gene": "rpmE", "product": "50S ribosomal protein L31", "uniref50_id": "UniRef50_P47499", "cog_id": "COG0254", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P47499", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47499" ], "product": "Large ribosomal subunit protein bL31" }, "genes": [ "rpmE" ], "id": "JPCNHPFPBM_581", "locus": "GCA000292505_00558" }, { "type": "cds", "sequence": "contig_1", "start": 307647, "stop": 308087, "strand": "+", "frame": 3, "gene": "prfA", "product": "peptide chain release factor 1", "db_xrefs": [ "COG:COG0216", "COG:J", "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149", "KEGG:K02835", "SO:0001217", "UniRef:UniRef50_P47500", "UniRef:UniRef90_P47500" ], "nt": "GTGGATTTTGACAAACAACTCTTTTTCAATGTTGAAAAGATTGTTGAACTTACTGAACAACTTGAAAAAGATCTCAATAAACCTAACCTTAGCTTTGAGCAGATTAAAGTTATTAACAAAGAGTTAAAACATAAACAACCTTTAATAGTTAAATTCAAGGAGTTGCAAAAGCTGGTTGAAAATGCTAATGAAGCTGAACAAATTCTTAACAATTCCAGCTTAAAAGAATTGCATGAAGAAGCTAAAAAAGAACTTGAAAAAATCAAAGCTAGTTTACCTAGTTTAGAAGAGGAGATTAAGTTCCTTTTACTACCGGTAGATGAAAATAACCAAAAGAATGTTATTGTAGAGATCCGTCCAGCTGCTGGTGGGGATGAATCTTGCATCTTCTTAAGTGATCTTTTTAATATGTACAAAAACTATTGTACTAGTAAAAATTGA", "aa": "MDFDKQLFFNVEKIVELTEQLEKDLNKPNLSFEQIKVINKELKHKQPLIVKFKELQKLVENANEAEQILNNSSLKELHEEAKKELEKIKASLPSLEEEIKFLLLPVDENNQKNVIVEIRPAAGGDESCIFLSDLFNMYKNYCTSKN", "aa_hexdigest": "79b475bf50048d446eca0dccdb6baad5", "start_type": "GTG", "rbs_motif": null, "genes": [ "prfA" ], "truncated": "3-prime", "pseudogene": { "start": 307647, "stop": 308390, "observations": { "mutation": [ 308085 ], "stop": [ 308085 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47500", "query_cov": 1.0, "subject_cov": 0.40668523676880225, "identity": 1.0, "score": 273.0, "evalue": 1.4e-89, "gene_start": 1, "gene_end": 146, "reference_start": 1, "reference_end": 146, "reference_sequence": "MDFDKQLFFNVEKIVELTEQLEKDLNKPNLSFEQIKVINKELKHKQPLIVKFKELQKLVENANEAEQILNNSSLKELHEEAKKELEKIKASLPSLEEEIKFLLLPVDENNQKNVIVEIRPAAGGDESCIFLSDLFNMYKNYCTSKNWTVELNEIIPASVGINFVSFAVNGTDVFAKLKFESGVHRVQRVPLTEAKGRVHTSTVTVAVLPQLEEVEITINPSDLRIDTYRASGAGGQHVNRTESAVRITHLPTGIVVACQEGKSQFSNRDKAMKMLRAKLWENAQNKQLSTQADLRKSQVGSGERAEKIRTYNYPQNRITDHRIKLTINKLNTVILGDLDEIIEALQADEKKQQLEKFIS" }, "paralog": false, "identity": 0.9919028340080972, "score": 475.0, "evalue": 1.21e-171, "description": "Internal stop codon at 308085. Nonsense mutation around 308085.", "psc": { "uniref90_id": "UniRef90_P47500", "gene": "prfA", "product": "peptide chain release factor 1", "uniref50_id": "UniRef50_P47500", "cog_id": "COG0216", "cog_category": "J", "kegg_orthology_id": "K02835", "go_ids": [ "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47500", "UniRef:UniRef50_P47500", "COG:COG0216", "COG:J", "KEGG:K02835", "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149" ] }, "pscc": { "uniref50_id": "UniRef50_P47500", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47500" ], "product": "Peptide chain release factor 1" } }, "id": "JPCNHPFPBM_582", "locus": "GCA000292505_00559" }, { "type": "cds", "sequence": "contig_1", "start": 308121, "stop": 308726, "strand": "+", "frame": 3, "gene": "prfA", "product": "peptide chain release factor 1", "db_xrefs": [ "COG:COG0216", "COG:J", "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149", "KEGG:K02835", "SO:0001217", "UniRef:UniRef50_P47500", "UniRef:UniRef90_P47500" ], "nt": "GTGGGGATTAACTTTGTTTCTTTTGCGGTAAATGGTACTGATGTTTTTGCTAAACTCAAGTTTGAATCAGGAGTACATAGGGTGCAACGTGTTCCTTTAACAGAAGCTAAAGGTAGAGTGCATACCTCAACAGTTACTGTTGCTGTTTTACCTCAATTAGAAGAGGTAGAGATCACCATTAATCCTAGTGATTTGAGAATCGATACTTATCGTGCTTCAGGAGCGGGTGGTCAACATGTTAACAGAACTGAAAGTGCAGTTAGAATTACCCATCTACCTACGGGAATTGTTGTGGCTTGCCAAGAAGGTAAATCCCAGTTTTCTAACCGTGATAAAGCAATGAAAATGCTACGTGCTAAGTTATGGGAAAATGCTCAAAATAAACAACTCTCAACCCAAGCGGATTTAAGAAAAAGTCAAGTTGGTAGTGGAGAGAGGGCTGAGAAAATTCGTACCTACAACTATCCCCAAAACAGAATTACAGACCACAGGATTAAATTAACTATTAATAAGCTTAATACTGTTATTTTGGGAGATCTTGATGAGATCATTGAAGCTTTACAAGCTGATGAAAAGAAACAACAGTTGGAAAAGTTTATTAGTTAG", "aa": "MGINFVSFAVNGTDVFAKLKFESGVHRVQRVPLTEAKGRVHTSTVTVAVLPQLEEVEITINPSDLRIDTYRASGAGGQHVNRTESAVRITHLPTGIVVACQEGKSQFSNRDKAMKMLRAKLWENAQNKQLSTQADLRKSQVGSGERAEKIRTYNYPQNRITDHRIKLTINKLNTVILGDLDEIIEALQADEKKQQLEKFIS", "aa_hexdigest": "c2cc6985177949825b26e2cd5375f320", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_K1ZX70", "query_cov": 0.9552238805970149, "subject_cov": 0.8421052631578947, "identity": 0.62, "score": 246.0, "evalue": 7.87e-80, "valid": false, "product": "Peptide chain release factor 1", "uniref50_id": "UniRef50_A0A2C9JR53", "kegg_orthology_id": "K02835" }, "genes": [ "prfA" ], "truncated": "5-prime", "pseudogene": { "start": 307821, "stop": 308726, "observations": { "mutation": [ 308085 ], "stop": [ 308085 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47500", "query_cov": 1.0, "subject_cov": 0.5598885793871866, "identity": 0.995, "score": 386.0, "evalue": 3e-133, "gene_start": 1, "gene_end": 201, "reference_start": 159, "reference_end": 359, "reference_sequence": "MDFDKQLFFNVEKIVELTEQLEKDLNKPNLSFEQIKVINKELKHKQPLIVKFKELQKLVENANEAEQILNNSSLKELHEEAKKELEKIKASLPSLEEEIKFLLLPVDENNQKNVIVEIRPAAGGDESCIFLSDLFNMYKNYCTSKNWTVELNEIIPASVGINFVSFAVNGTDVFAKLKFESGVHRVQRVPLTEAKGRVHTSTVTVAVLPQLEEVEITINPSDLRIDTYRASGAGGQHVNRTESAVRITHLPTGIVVACQEGKSQFSNRDKAMKMLRAKLWENAQNKQLSTQADLRKSQVGSGERAEKIRTYNYPQNRITDHRIKLTINKLNTVILGDLDEIIEALQADEKKQQLEKFIS" }, "paralog": false, "identity": 0.9966777408637874, "score": 583.0, "evalue": 3.63e-213, "description": "Internal stop codon at 308085. Nonsense mutation around 308085.", "psc": { "uniref90_id": "UniRef90_P47500", "gene": "prfA", "product": "peptide chain release factor 1", "uniref50_id": "UniRef50_P47500", "cog_id": "COG0216", "cog_category": "J", "kegg_orthology_id": "K02835", "go_ids": [ "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47500", "UniRef:UniRef50_P47500", "COG:COG0216", "COG:J", "KEGG:K02835", "GO:0003747", "GO:0005737", "GO:0006415", "GO:0016149" ] }, "pscc": { "uniref50_id": "UniRef50_P47500", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47500" ], "product": "Peptide chain release factor 1" } }, "id": "JPCNHPFPBM_583", "locus": "GCA000292505_00560" }, { "type": "cds", "sequence": "contig_1", "start": 308727, "stop": 308852, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCCTGTATGAGTTTTTTTTAAATCAAAAGTTAGTTTACCAATCCAGTCCCCATTTTAACGGGGTATTTTTAACAATATTGGAACACTATGGTTTTCAATTTAAAACAATTGATAAACTCTGA", "aa": "MTLYEFFLNQKLVYQSSPHFNGVFLTILEHYGFQFKTIDKL", "aa_hexdigest": "dbaef87e3742785d0b97ab90629f305c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4958.684300000001, "isoelectric_point": 6.697759056091309 }, "id": "JPCNHPFPBM_584", "locus": "GCA000292505_00561" }, { "type": "cds", "sequence": "contig_1", "start": 308934, "stop": 309209, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13649.12", "PFAM:PF05175.20" ], "nt": "TTGCCCTATTTGTTGGGAACAATTCAACTAAGGAAGCTTACTTTTAAAACTAAGAAAGGAGTTTTTATTCCTCGAATTGATAGCTTAGCACTAATTGCAAGTGTTAACTTAAAAAAAATAAAAACTGCACTTGACCTTTGTTGTGGTTCAGGTACTTTAGCCATTGCTTTAAAAAAGAAGTGTGATACACTTGATGTTTATGGTAGTGATATTGATATCCAAGCATTAAAACTAGCGCAACAAAATGCATTAATTAATAACGTTAGTATTAATTGA", "aa": "MPYLLGTIQLRKLTFKTKKGVFIPRIDSLALIASVNLKKIKTALDLCCGSGTLAIALKKKCDTLDVYGSDIDIQALKLAQQNALINNVSIN", "aa_hexdigest": "74af16969ad8a5c5c05b3ea180c1a804", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13649.12", "name": "Methyltransf_25", "length": 41, "aa_cov": 0.45054945054945056, "hmm_cov": 1.0, "evalue": 2.56428465349583e-08, "score": 29.63446044921875, "start": 44, "stop": 84 }, { "id": "PF05175.20", "name": "MTS", "length": 80, "aa_cov": 0.8461538461538461, "hmm_cov": 1.0, "evalue": 5.85326170926338e-13, "score": 43.91481399536133, "start": 13, "stop": 89 } ], "seq_stats": { "molecular_weight": 9890.738899999991, "isoelectric_point": 9.585433387756346 }, "id": "JPCNHPFPBM_585", "locus": "GCA000292505_00562" }, { "type": "cds", "sequence": "contig_1", "start": 309336, "stop": 309767, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTTTTCAAAATAAAAATAGTTATTTTGCATACAAGCAGTTGTTTAATCTATTACTAACAAAACGATCAATTAAACAGTTAATTTTTGAATGTTCTTTATTTCAAAAAGAAAGGCTATTAAATTTGTTTTCAATCTTTAAATCAAGGCCGATTTTTAACTTTCAAAAACAGTTTATTGGTATGAAAGTTGATAATCAAAAACTCCCAGTAGTTGATATTAAAAATACCAAAACTATTAAGCAACTTTTAAAAATGGGGCTAGCAGGAATTGTAAATACTGATACACAAATGGGATTAATTAGTTATTCAGAGTCTACTCTTGACAAAATTAAACAACGTGCACTTAACAAACATTATGTATCAATGTTTGGGTTAGAAGAATTAAAGAAGTTACCAAAAAAACTACAACAAATTGCTAGTTACTTTTGA", "aa": "MVFQNKNSYFAYKQLFNLLLTKRSIKQLIFECSLFQKERLLNLFSIFKSRPIFNFQKQFIGMKVDNQKLPVVDIKNTKTIKQLLKMGLAGIVNTDTQMGLISYSESTLDKIKQRALNKHYVSMFGLEELKKLPKKLQQIASYF", "aa_hexdigest": "0149733c06491adca5ee57a3bf28c3e5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16795.792999999983, "isoelectric_point": 10.03767910003662 }, "id": "JPCNHPFPBM_586", "locus": "GCA000292505_00563" }, { "type": "cds", "sequence": "contig_1", "start": 310228, "stop": 310863, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGCTCAAAGGTTTTTTAGCTGTTGGTGTTAGTGTTTTTGGTTTTTCTGGTTTACTGATGGCTTGTAGTGTTGTAAGTCAGTTTGATCAAGTGGATGATGGCAAAATTAAGCTAGCATCTTCATTAACTTCGAAACGCGCAGCGGAAGCTTTAGAGACAGTAGTTAAAAAATATAACGATACTAAAGATCCTGGTGATTATCCAATTGAAATAGTACAAATTGCTGGGGGTTATGATGGGGGTAAAAAGGATGTTCAAACCAAGGTTAGTACCAAAGATAAAAATAACTTTTATAACCTGATTTTAAACTATCCAGAAATAGTAAGTACCTTATCAAGGTCTAAAATGGCTTTGAATTTTGATGGGGTTAATGTTGATAAACTCCATCCTAACTTTTTAAGCTTTAACAGCAGAATAGGTGGAATTAGAGATGATGGAATCTATGCTATTCCAATATCTATGTCCACTGATCTGATGGTCATTAATGGCCCTGTTTTACACTATATTCTAAACAGTGCTAGAAAAGAAGGTACACCAACTAGCACTACTGTTCAAGCAACTGTCAGTTCAAGAAGTGCAGAAAAAAAAGGTACATTAGAAATTGCAAATGATAGTGAAACTACTAAACTTTGA", "aa": "MKLKGFLAVGVSVFGFSGLLMACSVVSQFDQVDDGKIKLASSLTSKRAAEALETVVKKYNDTKDPGDYPIEIVQIAGGYDGGKKDVQTKVSTKDKNNFYNLILNYPEIVSTLSRSKMALNFDGVNVDKLHPNFLSFNSRIGGIRDDGIYAIPISMSTDLMVINGPVLHYILNSARKEGTPTSTTVQATVSSRSAEKKGTLEIANDSETTKL", "aa_hexdigest": "dfe8ca7c1eda06638be0967a630571ae", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22836.78190000001, "isoelectric_point": 8.499463462829592 }, "id": "JPCNHPFPBM_587", "locus": "GCA000292505_00564" }, { "type": "cds", "sequence": "contig_1", "start": 311380, "stop": 312525, "strand": "+", "frame": 1, "gene": null, "product": "P68 family surface lipoprotein", "db_xrefs": [ "GO:0005886", "GO:0009897", "SO:0001217", "UniRef:UniRef50_P75280", "UniRef:UniRef90_P47502" ], "nt": "TTGTTTGTTAACCGTGGTGGTTCCTATTCATCCAACTTTGAAAAATTTCACCAATTGGCATTCTCAGTATCTTCTTCTGGAGGATACAGTTATTACTTTGCTAAAGATAATGCTAAGCGCTTAAAGTTTAGTAATTATGCTATTGAATATCCTAGTTTTACCCAAACAATTCAAGCTCCTAATTCTTCAGAAACAGAAAGTAATTTACTTGGTACTTTTAAATTAAGTGAAAAAGATATCAATCTATATAAAGGTTCAATTCCTAGTGGAAAACAACAAGGAGTTGATGCTATCTTAATTAGTAACCCAAACTTAATTAATATTCTTGAACAAGCAAAACAAAAAAACACTGCACAAGGAAGTGAATCAACCACTAACAAGATAATAGGTTATACCACCACTGCAAATGTTAATGTTGATAATCAAAACATCTTTTCTGTTAGCAAACTTAACAACGAACAGTTTCAAAGAAAAATCATTGTTAATGCCACTGAAGAAACACTTGATCAATCCCAAACCTTACAGAGCAATGAATCAATTGTTTTACCAATGCCTGGTAAATACAAATCAACTGATAAAAATAAAGTAATGATCACCCAAGGTCCTAACTTAATAGGCATCCATGCAAATGAAAAAGAAAATATTGAAACTAAAAAATTTGTTAATTGGTTTTTAAATCAAAGTATTACAGATTGGAATAGTAATAATCAGCAAAAAAATAGTGATCAAACAACAAAAACTGCTGCTGAATATTTCACTGATCAAGCTTCTTACATCCTTCCTTTAAAGGAAAAATTTAACAAAAGTTCAGATTTAGAATTGAAAGGCAGTAGTAGTTCTTCTAATTTAACAACCAGTAGTGCTAGCGCCTCTTTGTTAATAAGTAATAATAGTTCAACTGCAAGTTCTCCTGCTCCTAAAAAAACAAACAATAATTCTAATACCTTTACAGCTAAAGCACTAGAATTATTCCAACAAGCTGCTAACAATGAAATTATTCCCTTTAGTGATCCAAGTGACTTTAGGAACGGCACATTCCGTAATAATATAAGCAGTAGTTTTAATGCTGCGGTGAATTCTAAGGTTAGTTTTAATCAATTTGTCCAAAACTTTATTAATAGTTTAGGATCTGGATTTAGAAGATAA", "aa": "MFVNRGGSYSSNFEKFHQLAFSVSSSGGYSYYFAKDNAKRLKFSNYAIEYPSFTQTIQAPNSSETESNLLGTFKLSEKDINLYKGSIPSGKQQGVDAILISNPNLINILEQAKQKNTAQGSESTTNKIIGYTTTANVNVDNQNIFSVSKLNNEQFQRKIIVNATEETLDQSQTLQSNESIVLPMPGKYKSTDKNKVMITQGPNLIGIHANEKENIETKKFVNWFLNQSITDWNSNNQQKNSDQTTKTAAEYFTDQASYILPLKEKFNKSSDLELKGSSSSSNLTTSSASASLLISNNSSTASSPAPKKTNNNSNTFTAKALELFQQAANNEIIPFSDPSDFRNGTFRNNISSSFNAAVNSKVSFNQFVQNFINSLGSGFRR", "aa_hexdigest": "6b187ebffdb5d07938715c5c646df684", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 311080, "stop": 312525, "observations": { "mutation": [ 311083 ], "stop": [ 311083 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47502", "query_cov": 1.0, "subject_cov": 0.4980392156862745, "identity": 0.997, "score": 647.0, "evalue": 8.33e-227, "gene_start": 1, "gene_end": 381, "reference_start": 385, "reference_end": 765, "reference_sequence": "MKLKGFLAVGVSVFGFSGLLMACSVVSQFDQVDDGKIKLASSLTSKRAAEALETVVKKYNDTKDPGDYPIEIVQIAGGYDGGKKDVQTKVSTKDKNNFYNLILNYPEIVSTLSRSKMALNFDGVNVDKLHPNFLSFNSRIGGIRDDGIYAIPISMSTDLMVINGPVLHYILNSARKEGTPTSTTVQATVSSRSAEKKGTLEIANDSETTKLWQNIQTTAQNNSNETTKEQKQVKRSSSSSSTTSTTGETKDTTKSDNKIKEFWGEYQEVDGGLKNFTFKASIFENWNETLDFATRIANSFPEKVKNITNKTGLDLQGVLGVDSSSNALYAAVFAAGQANYDNFFFNIDKRTGYADYSNFLNKDSSYQNLESVYNDFYKLIQANGLFVNRGGSYSSNFEKFHQLAFSVSSSGGYSYYFAKDNAKRLKFSNYAIEYPSFTQTIQAPNSSETESNLLGTFKLSEKDINLYKGSIPSGKQQGVDAILISNPNLINILEQAKQKNTAQGSESTTNKIIGYTTTANVNVDNQNIFSVSKLNNEQFQRKIIVNATEETLDQSQTLQSNESIVLPMPGKYKSTDKNKVMITQGPNLIGIHANEKENIETKKFVNWFLNQSITDWNSNNQQKNSDQTTKTAAEYFTDQASYILPLKEKFNKSSDLELKGSSSSSNLTTSSASASLLISNNSSTASSPAPKKTNNNSNTFTAKALELFQQAANNEIIPFSDPSDFRNGTFRNNISSSFNAAVNSKVSFNQFVQNFINSLGSGFRR" }, "paralog": false, "identity": 0.997920997920998, "score": 867.0, "evalue": 4.11e-316, "description": "Internal stop codon at 311083. Nonsense mutation around 311083.", "psc": { "uniref90_id": "UniRef90_P47502", "product": "P68 family surface lipoprotein", "uniref50_id": "UniRef50_P75280", "go_ids": [ "GO:0005886", "GO:0009897" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47502", "UniRef:UniRef50_P75280", "GO:0005886", "GO:0009897" ] }, "pscc": { "uniref50_id": "UniRef50_P75280", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75280" ], "product": "Uncharacterized lipoprotein MPN_506" } }, "id": "JPCNHPFPBM_588", "locus": "GCA000292505_00565" }, { "type": "cds", "sequence": "contig_1", "start": 312554, "stop": 312910, "strand": "+", "frame": 2, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7M3VDH2", "UniRef:UniRef90_UPI001EE65182" ], "nt": "GTGTTACTAGTGCCTAAGGTGGTAGTGGAGTACCACAAGCTCAGTAAGAAGGTTGTCAAAGAGAGTTTGGAAGTGGAAGCAACTGATTCCTTTGATCCCACCCAAAGGTTGCAAAAAGATAGTCCAATGAAGGATTCAACAAAAACAGGGGAGAAACTCCAAGAGACCATGTCATCAATGAGTGGTATGGCTACATCCACTCGCAAGGCCCTCAAAGTGGAGGTGGAAAGGGGGAGTAAAGTCAATCAAGGCGAACTAGCAAAAAACGACTTTGCTAAAAAGCCACTGAAACATAAAGAAAATAGTGGGGAAGTGAAGTTAGATGCAGAGAAGGATTTTACTGAAGGAAAGGTTTGA", "aa": "MLLVPKVVVEYHKLSKKVVKESLEVEATDSFDPTQRLQKDSPMKDSTKTGEKLQETMSSMSGMATSTRKALKVEVERGSKVNQGELAKNDFAKKPLKHKENSGEVKLDAEKDFTEGKV", "aa_hexdigest": "d73dde571d88336447def87497572fbd", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030D24C90", "query_cov": 0.9322033898305084, "subject_cov": 0.8661417322834646, "identity": 0.7909999999999999, "score": 147.0, "evalue": 1.45e-43, "valid": false, "product": "adhesin", "uniref50_id": "UniRef50_A0A7M3VDG5" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 312563, "stop": 313103, "observations": { "deletion": [ 312956, 313046 ], "mutation": [ 312908 ], "stop": [ 312908, 312980, 313088 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001EE65182", "query_cov": 0.9745762711864406, "subject_cov": 0.6845238095238095, "identity": 0.878, "score": 180.0, "evalue": 6.09e-56, "gene_start": 5, "gene_end": 118, "reference_start": 2, "reference_end": 116, "reference_sequence": "MPKVVVEYHQLDKKVVKESLEVEATSTFNPTQRLKSDSPMKDSTKTGEKLQETMSSMSGMATSTRKALKVEVERGSNVNQGELAKNDFAKKPLKHKNSSGTDVKLDSQKDFTEGKVWKPVLTTDEIKKNRGMGAFANSPSPSTSASSLPHSPLFLTSMLGLNQWSLNT" }, "paralog": false, "identity": 0.8066298342541437, "score": 260.0, "evalue": 3.14e-90, "description": "Internal stop codon at 312908, 312980, 313088. Frameshift due to deletion around 312956, 313046. Nonsense mutation around 312908.", "psc": { "uniref90_id": "UniRef90_UPI001EE65182", "product": "adhesin", "uniref50_id": "UniRef50_A0A7M3VDH2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001EE65182", "UniRef:UniRef50_A0A7M3VDH2" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7M3VDH2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7M3VDH2" ], "product": "MgpB adhesin protein" } }, "id": "JPCNHPFPBM_589", "locus": "GCA000292505_00566" }, { "type": "cds", "sequence": "contig_1", "start": 313215, "stop": 313598, "strand": "+", "frame": 3, "gene": null, "product": "Random genomic clone esh10, partial cds", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49206" ], "nt": "GTGAAGCCTAGTAGTAGTAGTAACTCGTTTAATCCCTCCTCTGCTGAAAATCAAGTCACCCCAGCTGGTGGCTCCTCCAAACCAACCACCTACACCCATTTACCCAACAGTATCAGTCCCACCAGTGACTGGATCAACGCATTAACCTTCACCAATAAGAATAATCCGCAGCGCAATCAGTTGTTGTTAAGAGCACTGTTAGGAACTATCCCGGTCTTGATCAATAAGAGTGGAAAGGGAGAAGAATTTACCAAAGATAGTGAGCAGAAGTGGGATAAAACGAATAAAAAAGATGGGAATTTACCGGGGTTTGGGGAGGTGAATGGTGCTTCTTATAAGATTTTTACTTATTTAATAATTAAAAAAAGTGTTAGGTTTTTTTAG", "aa": "MKPSSSSNSFNPSSAENQVTPAGGSSKPTTYTHLPNSISPTSDWINALTFTNKNNPQRNQLLLRALLGTIPVLINKSGKGEEFTKDSEQKWDKTNKKDGNLPGFGEVNGASYKIFTYLIIKKSVRFF", "aa_hexdigest": "a307f21afbb5a20580127a0c2181cc49", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00027B3795", "query_cov": 0.905511811023622, "subject_cov": 0.9349593495934959, "identity": 0.861, "score": 186.0, "evalue": 1.08e-58, "valid": false, "product": "adhesin", "uniref50_id": "UniRef50_Q49206" }, "pscc": { "uniref50_id": "UniRef50_Q49206", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49206" ], "product": "Random genomic clone esh10, partial cds" }, "genes": [], "id": "JPCNHPFPBM_590", "locus": "GCA000292505_00567" }, { "type": "cds", "sequence": "contig_1", "start": 314064, "stop": 314240, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13", "UniRef:UniRef90_UPI002FCE54A6" ], "nt": "GTGGTGAAAAAATATCAGTTAGATGTACCAAATGTTACCAATGAGATGAACGATGTACTGAAAGAGTTGATCCTTGAACAACCTTTGACTAAGTATACCTTAAATTCGAGTTTGGCCAAAGAAAAGGGTAAGACACAAGCAGTGGTGCATCTTGGTTCAAATATGGCAAATCAGTGA", "aa": "MVKKYQLDVPNVTNEMNDVLKELILEQPLTKYTLNSSLAKEKGKTQAVVHLGSNMANQ", "aa_hexdigest": "73683537ae72a85bbd603704ced66d7c", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 313923, "stop": 314305, "observations": { "deletion": [ 314259 ], "mutation": [ 314058, 314238 ], "stop": [ 314058, 314238 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FCE54A6", "query_cov": 0.896551724137931, "subject_cov": 0.41935483870967744, "identity": 0.846, "score": 90.9, "evalue": 2.64e-22, "gene_start": 1, "gene_end": 52, "reference_start": 47, "reference_end": 98, "reference_sequence": "MLFKSTQVKLTYSSSGSNNISFENNANKPSYVVEFTNSTTVGIKWSVVKKYQLDVPNVTNEMNQVLQELILEQPLTKYTLNGSLAKQKGKSQVAVHLGGQASQWHSTRHSQSKQQSQPQGFNRI" }, "paralog": false, "identity": 0.7559055118110236, "score": 166.0, "evalue": 1.22e-54, "description": "Internal stop codon at 314058, 314238. Frameshift due to deletion around 314259. Nonsense mutation around 314058, 314238.", "psc": { "uniref90_id": "UniRef90_UPI002FCE54A6", "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FCE54A6", "UniRef:UniRef50_UPI00027B3D13" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6516.476900000003, "isoelectric_point": 8.030198097229004 }, "id": "JPCNHPFPBM_591", "locus": "GCA000292505_00568" }, { "type": "cds", "sequence": "contig_1", "start": 314356, "stop": 314568, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACCGATTGATGGGACCAAACAGGGCAAAGGGAAGGATAGTAGTGGGTGGAATTCAACTGAAGAAAATACGGCAGCAGGGGATGCGCCCCTTGTTTCAACAAGTGGAACATCAGATCAAAGTAATAAATTCACCAAGTACCTCAACACCAAGCAAGCATTGGCAAGCATCGGCATCTTGTTTGATGGAAATGGGCGAGGAATGTGGTTAG", "aa": "MNRLMGPNRAKGRIVVGGIQLKKIRQQGMRPLFQQVEHQIKVINSPSTSTPSKHWQASASCLMEMGEECG", "aa_hexdigest": "85c550dd8ec4504cbb0be7cf7ff9d9c7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7806.069200000003, "isoelectric_point": 10.192338752746583 }, "id": "JPCNHPFPBM_592", "locus": "GCA000292505_00569" }, { "type": "cds", "sequence": "contig_1", "start": 314561, "stop": 314839, "strand": "+", "frame": 2, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FCAC30C" ], "nt": "GTGGTTAGCCATCTCTACCAACCCAACAAGGTGAAAAGTGGTCAATATCAACAAAATAACACCTACAACAAGTTAATTGAACCGGAAAGTGCAACAAGTGCAGCAACTAACATGACCAACTTGTTAAAGCTGTTGTCTAGTAAAAACATCAAACAGAAGTTGGGGAAGACAGCCCAATCTCAGGAAAATAGTGGGGGGGGGTTAGTCAAACGATTAACACCATTACCACTACGGGAAATATTAGTGGCAATGGAACCATTAAAACGGCTTATCCGGTGA", "aa": "MVSHLYQPNKVKSGQYQQNNTYNKLIEPESATSAATNMTNLLKLLSSKNIKQKLGKTAQSQENSGGGLVKRLTPLPLREILVAMEPLKRLIR", "aa_hexdigest": "a251768e60bbbef62237bbe4257c2831", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00027B3797", "query_cov": 1.076086956521739, "subject_cov": 0.908256880733945, "identity": 0.6459999999999999, "score": 100.0, "evalue": 7.76e-26, "valid": false, "uniref50_id": "UniRef50_UPI001FCAC30C" }, "pscc": { "uniref50_id": "UniRef50_UPI001FCAC30C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001FCAC30C" ], "product": "adhesin" }, "genes": [], "id": "JPCNHPFPBM_593", "locus": "GCA000292505_00570" }, { "type": "tRNA", "sequence": "contig_1", "start": 315006, "stop": 315082, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(tcg)", "amino_acid": "Arg", "anti_codon": "tcg", "score": 73.0, "nt": "GCGCCCATAGCTCAATCGGATAGAGTGTCTGGCTTCGGACCAGAAGGTTATGGGTTCAAGTCCTATTGGGCGCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 315065, 315067 ], "id": "JPCNHPFPBM_31", "locus": "GCA000292505_00571" }, { "type": "cds", "sequence": "contig_1", "start": 315172, "stop": 315402, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MPN_377", "db_xrefs": [ "RefSeq:WP_009885892.1", "SO:0001217", "UniParc:UPI0000557E10", "UniRef:UniRef100_UPI0000557E10", "UniRef:UniRef50_Q9EXD3", "UniRef:UniRef90_UPI0000557E10" ], "nt": "ATGGCAAAAGATAAAAAAAATAAGGTTGATGGAACAGAGCAATCAGTTGATCTATTTGAACGTACAAAACTTGAAGATACACAAGTTTTAAATGAAGTTGAACTTGATGATATTAAAAAGATAACAGAGCTTAGAAAAGAACTTGAACATACTTTTGAACCACAAACAAGAATGCAAATTAAGCGTGAAATTAAAGAAATAGAACGCAAAATGAAACGTTCTAGTCGCTAA", "aa": "MAKDKKNKVDGTEQSVDLFERTKLEDTQVLNEVELDDIKKITELRKELEHTFEPQTRMQIKREIKEIERKMKRSSR", "aa_hexdigest": "216d36a3184e8aa2b7fdf2f737d21396", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000557E10", "ncbi_nrp_id": "WP_009885892.1", "uniref100_id": "UniRef100_UPI0000557E10", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000557E10", "RefSeq:WP_009885892.1", "UniRef:UniRef100_UPI0000557E10" ] }, "ips": { "uniref100_id": "UniRef100_UPI0000557E10", "uniref90_id": "UniRef90_UPI0000557E10" }, "psc": { "uniref90_id": "UniRef90_UPI0000557E10", "uniref50_id": "UniRef50_Q9EXD3" }, "pscc": { "uniref50_id": "UniRef50_Q9EXD3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9EXD3" ], "product": "Uncharacterized protein MPN_377" }, "genes": [], "id": "JPCNHPFPBM_594", "locus": "GCA000292505_00572" }, { "type": "cds", "sequence": "contig_1", "start": 315406, "stop": 315768, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGTTAATTTACATACAAATTCATACTATAACTTTCTCAATTCTGCCCTTTCTCCTAAAAAGCTAGTTAATCTAGCAATTAATGATCAGCAAAAAGCTGTTGCTATTACAGATCCTAATCTTTTTGGCGCTGTTGAATTTTTTATAACTTGTAAGCAAAATAATATTAAACCAATTATTGGTTTAAACTTAACTGTTGAATACCAAAAAAATGATGTTAAGTTATTACTAATTGCTAAATCAAATAAAGGCTTTCAAACGTTGAACAAAATAGCATTAATTCAACAAAAACTTGAAATTAATTCTTTAGTTGATCAACTAACAGATATTGCAGTAATTATCTGTTCTTTAACAACATGA", "aa": "MFVNLHTNSYYNFLNSALSPKKLVNLAINDQQKAVAITDPNLFGAVEFFITCKQNNIKPIIGLNLTVEYQKNDVKLLLIAKSNKGFQTLNKIALIQQKLEINSLVDQLTDIAVIICSLTT", "aa_hexdigest": "a11942cdc52a670fb4f4b451b90467ff", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13410.536799999982, "isoelectric_point": 8.928629493713384 }, "id": "JPCNHPFPBM_595", "locus": "GCA000292505_00573" }, { "type": "cds", "sequence": "contig_1", "start": 315901, "stop": 317013, "strand": "+", "frame": 1, "gene": "dnaE", "product": "DNA polymerase III subunit alpha", "db_xrefs": [ "COG:COG0587", "COG:L", "EC:2.7.7.7", "GO:0003887", "GO:0005737", "GO:0006260", "GO:0008408", "SO:0001217", "UniRef:UniRef50_Q49405", "UniRef:UniRef90_Q49405" ], "nt": "ATGAAACAAAACCAAACGGGAAAAATAACTACATTTGATTGGGATTTTAAACAAAAATTAAATCAAATTTCAATTAATGAAAATTTAAAAGTAAAGAGTGAAATTCAACCTTTTTTAGATCAAAAAACTGCACAACAATTATTCAGTGAAACAGAACTTAATAATCTGAATGATCTAGTTAATAGATGTGAATTAGATTTGGAGCACCTAAAAGCTGCTTCACTTTCTTTAACTGATAATGATGCAGCAGTTTTAGAAAGTTTGTGCCAAACCAATTTAAAACAGTTTTTAGATAAAAATCAAGATCTAAATAAAAAAGCCTATCAGCTACGTTTAGAGAAGGAATTAAATGTTATCAATAAACTTAATTTTGCTAGCTATTTTTTAGTTGTCAATGATCTTGTTAATTATGCTTTTAAAAAGGACATCTTAATTGGTTCTGGTAGAGGTTCTGCAGTAGGATCATTAGTGGCTTTTTTATTAAACATTACCAAGATAGACCCAGTCCAACACCAGCTTATTTTCGAACGTTTTATCTCAACCCACCGTCAAGATCTACCTGATATTGATATTGATATCATGGAGAATAAAAGAGCAGAAATGATAAATTATCTGTTTGAAAAATATGGCAAAGAAAACTGTGCACAAATTGTTACTTTTCAACGTTTTAAAACCCGTTCTGCTGTTAAAGAAGTTGCTAAATTATTTAATGATTATGGCATTAGTGACATGATCCTAGGAGTGTTACCTAAAGATCAAACTATAACATTCACTGAGCTTAAAGCTACTGAAGATAGTGCTTTACAACTTTGTTTACAACAGTTTGGTTTAATTGTTGAATTAGCACTAGCAATAGTTGATTTTCCAAGACAATCAAGTATCCATGCTTCAGGCATAGTTATCGCTTCAAATTCTTTGATTAAAACCATTCCCTTGTTACAGCTTGACAATAATCACTTTTTAACTCAAGTTTCAATGGAATGGTTAAGTTTTTTTAATCTCAATAAGTTTGATCTGCTTGGTTTAATTAACCTTACTATGATTAGCGATGTAATTACCCAAATTAAACCATCTAACCAGACCGTTAACCAGTTTTTAAATACCATTTCTTGA", "aa": "MKQNQTGKITTFDWDFKQKLNQISINENLKVKSEIQPFLDQKTAQQLFSETELNNLNDLVNRCELDLEHLKAASLSLTDNDAAVLESLCQTNLKQFLDKNQDLNKKAYQLRLEKELNVINKLNFASYFLVVNDLVNYAFKKDILIGSGRGSAVGSLVAFLLNITKIDPVQHQLIFERFISTHRQDLPDIDIDIMENKRAEMINYLFEKYGKENCAQIVTFQRFKTRSAVKEVAKLFNDYGISDMILGVLPKDQTITFTELKATEDSALQLCLQQFGLIVELALAIVDFPRQSSIHASGIVIASNSLIKTIPLLQLDNNHFLTQVSMEWLSFFNLNKFDLLGLINLTMISDVITQIKPSNQTVNQFLNTIS", "aa_hexdigest": "ba9cec84d89fb5e23f79e197a3137362", "start_type": "ATG", "rbs_motif": null, "genes": [ "dnaE" ], "truncated": "both", "pseudogene": { "start": 315601, "stop": 317316, "observations": { "mutation": [ 315766, 317011 ], "stop": [ 315766, 317011 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49405", "query_cov": 1.0, "subject_cov": 0.4233409610983982, "identity": 0.995, "score": 708.0, "evalue": 1.55e-249, "gene_start": 1, "gene_end": 370, "reference_start": 166, "reference_end": 535, "reference_sequence": "MFVNLHTNSYYNFLNSALSPKKLVNLAINDQQKAVAITDPNLFGAVEFFITCKQNNIKPIIGLNLTVEYQKNDVKLLLIAKSNKGFQTLNKIALIQQKLEINSLVDQLTDIAVIICSLTTWKSTYKDVYQAKGIEINQTPIAILANAVNCEKTNSDQVVLTVLKQMKQNQTGKITTFDWDLKQKLNQISINENLKVKSEIQPFLDQKTAQQLFSETELNNLNDLVNRCELDLEHLKAASLSLTDNDAAVLESLCQTNLKQFLDKNQDLNKKAYQLRLEKELNVINKLNFASYFLVVNDLVNYAFKKDILIGSGRGSAVGSLVAFLLNITKIDPVQHQLIFERFISTHRQDLPDIDIDIMENKRAEMINYLFEKYGKENCAQIVTFQRFKTRSAVKEVAKLFNDYGISDMILGVLPKDQTITFTDLKATEDSALQLCLQQFGLIVELALAIVDFPRQSSIHASGIVIASNSLIKTIPLLQLDNNHFLTQVSMEWLSFFNLNKFDLLGLINLTMISDVITQIKPSNQTVNQFLNTISWTDQNTFINLVNEDTLGIFQLESFGMKKLLVQIKPKTINQLAIVLALYRPGAQDNINLFINRLHNGYDQSDIDPRILPIVKNTYGVLIFQEQIINIVKVVANYSLEEADSFRRAISKKDVKLIQKNKRNFFERAVQNNFDLKTTTKIFSYIERFANYGFNLSHALGYALLSYWTAWLKTNYPVYFYLWLLNHFQSSKDKQKLIIRTLEKSGIEIYPPLLNKAQPNSVIENKKIYLGLNLIKGINDRYIQNLQKVQHLIQTQNNLQLTDVVSWCLDKTIGDIPLKDLLLLKTMGCFDFFEYTYDFNDAKDFWIKSDHLLFTRMPLEKKDSNFWIKQFFTN" }, "paralog": false, "identity": 0.9929947460595446, "score": 1094.0, "evalue": 0.0, "description": "Internal stop codon at 315766, 317011. Nonsense mutation around 315766, 317011.", "psc": { "uniref90_id": "UniRef90_Q49405", "gene": "dnaE", "product": "DNA polymerase III subunit alpha", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_Q49405", "cog_id": "COG0587", "cog_category": "L", "go_ids": [ "GO:0005737", "GO:0008408", "GO:0003887", "GO:0006260" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49405", "EC:2.7.7.7", "UniRef:UniRef50_Q49405", "COG:COG0587", "COG:L", "GO:0005737", "GO:0008408", "GO:0003887", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_Q49405", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49405" ], "product": "DNA polymerase III subunit alpha" } }, "id": "JPCNHPFPBM_596", "locus": "GCA000292505_00574" }, { "type": "cds", "sequence": "contig_1", "start": 317086, "stop": 317529, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17657.6" ], "nt": "ATGAAAAAATTACTGGTTCAGATTAAACCTAAAACCATTAATCAACTAGCAATTGTTCTAGCGCTTTACAGACCAGGTGCACAGGATAACATTAACCTTTTTATTAACCGTTTGCACAATGGTTATGATCAATCTGACATTGATCCTAGGATTTTACCCATTGTGAAAAATACCTATGGAGTTTTAATTTTTCAAGAGCAGATCATTAACATCGTTAAAGTTGTGGCTAACTACTCTTTAGAAGAAGCAGATAGCTTCCGTAGAGCCATTTCTAAAAAGGATGTTAAATTGATCCAAAAAAATAAGCGTAACTTCTTTGAAAGAGCAGTTCAAAATAACTTTGATTTAAAGACTACTACCAAAATTTTTAGCTACATAGAACGCTTTGCTAACTATGGGTTTAACCTTTCTCATGCGTTGGGTTATGCACTGCTTTCATACTGA", "aa": "MKKLLVQIKPKTINQLAIVLALYRPGAQDNINLFINRLHNGYDQSDIDPRILPIVKNTYGVLIFQEQIINIVKVVANYSLEEADSFRRAISKKDVKLIQKNKRNFFERAVQNNFDLKTTTKIFSYIERFANYGFNLSHALGYALLSY", "aa_hexdigest": "a1a9a9b13fbce59c5aa0dabbc9c84b39", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17657.6", "name": "DNA_pol3_finger", "length": 115, "aa_cov": 0.7687074829931972, "hmm_cov": 1.0, "evalue": 1.2080360115603936e-36, "score": 120.6449966430664, "start": 1, "stop": 113 } ], "seq_stats": { "molecular_weight": 17055.596099999988, "isoelectric_point": 9.785091972351072 }, "id": "JPCNHPFPBM_597", "locus": "GCA000292505_00575" }, { "type": "cds", "sequence": "contig_1", "start": 317770, "stop": 317943, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAACATTTAATTCAAACTCAAAATAACTTACAACTAACTGATGTAGTAAGTTGGTGTTTGGATAAAACCATTGGTGATATCCCTTTAAAAGATTTACTTTTATTAAAAACTATGGGCTGTTTTGATTTTTTTGAATACACTTATGACTTTAATGATGCAAAGGATTTTTGA", "aa": "MQHLIQTQNNLQLTDVVSWCLDKTIGDIPLKDLLLLKTMGCFDFFEYTYDFNDAKDF", "aa_hexdigest": "69c2fb9a3f3b43dc08c1018e39d14ff5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6720.630399999998, "isoelectric_point": 4.222591590881348 }, "id": "JPCNHPFPBM_598", "locus": "GCA000292505_00576" }, { "type": "cds", "sequence": "contig_1", "start": 318157, "stop": 318912, "strand": "+", "frame": 1, "gene": "polA", "product": "5'-3' exonuclease", "db_xrefs": [ "COG:COG0258", "COG:L", "EC:3.1.11.-", "GO:0003677", "GO:0008409", "GO:0017108", "GO:0033567", "SO:0001217", "UniRef:UniRef50_P75403", "UniRef:UniRef90_Q49406" ], "nt": "ATGTTACTAATGTGTTGGAATTTAATTAAAGCCAATGTTTATCAATATGGAATTGTAAGTTTTGACACTAAAGCGCCAACTTTCCGTGATCAAATCTATGAAGGATATAAACAAAAAAGGGTTAAAACTCCAGTTGAACTTTTAGTACAAATTCCTCTAATTAAACAAGCGCTTGTTTATTTAGGGTTTTTAGTTTGTGAAAAAGATGGCTTTGAAGCAGATGATTTGATTGGTAGTTATGCCAATTTATTTACAAAGCAAGAAATAACAGTTGATATTTACAGTTCAGATCGAGATATGTTGCAATTAGTAAACGCCTTTACTAATGTGTTTCTCTGTGTTAAAGGTACAAAGGAGATGGTTATGTACAACAATGAAAATTTTAAATCACTTTTTTATGGTTTGGCGCCCTATCAAGTTGTTGAATATAAGGGGTTAGTTGGTGATAACAGCGATAATTTAGCAGGGATTAAAGGGATAGGTCCCATCAAAGGGATAGAATTACTCCAACAATATGGAACCATTGATAACATCTACACTAACTTCAATAATCTCCCCAACCAACTTCAAAAACTTTTAAATAACCAAAAGGAAATAGCTAAAACCTTTAGTTTTCTAGCTAAAATTAAAACTGATATTGAACTTGATCAAAACATAGATCTTACTGGTTTAAAACCAATCCAAAAACAAGCGTTAATTCAACTTCTAAGTGAAAACAAAATTAATACTTTAGTTGAAAAATTTTCAAAAATATAA", "aa": "MLLMCWNLIKANVYQYGIVSFDTKAPTFRDQIYEGYKQKRVKTPVELLVQIPLIKQALVYLGFLVCEKDGFEADDLIGSYANLFTKQEITVDIYSSDRDMLQLVNAFTNVFLCVKGTKEMVMYNNENFKSLFYGLAPYQVVEYKGLVGDNSDNLAGIKGIGPIKGIELLQQYGTIDNIYTNFNNLPNQLQKLLNNQKEIAKTFSFLAKIKTDIELDQNIDLTGLKPIQKQALIQLLSENKINTLVEKFSKI", "aa_hexdigest": "e90c38a59260d249dc7c3810d187c4bb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q49406", "query_cov": 1.0, "subject_cov": 0.8625429553264605, "identity": 0.996, "score": 494.0, "evalue": 3.38e-176, "valid": true, "gene": "polA", "product": "5'-3' exonuclease", "ec_ids": [ "3.1.11.-" ], "uniref50_id": "UniRef50_P75403", "cog_id": "COG0258", "cog_category": "L", "go_ids": [ "GO:0008409", "GO:0017108", "GO:0003677", "GO:0033567" ] }, "pscc": { "uniref50_id": "UniRef50_P75403", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75403" ], "product": "5'-3' exonuclease" }, "genes": [ "polA" ], "id": "JPCNHPFPBM_599", "locus": "GCA000292505_00577" }, { "type": "cds", "sequence": "contig_1", "start": 318912, "stop": 319766, "strand": "+", "frame": 3, "gene": "mutM", "product": "DNA-formamidopyrimidine glycosylase", "db_xrefs": [ "COG:COG0266", "COG:L", "EC:3.2.2.23", "GO:0003684", "GO:0003906", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0008270", "GO:0008534", "GO:0034039", "GO:0046872", "GO:0140078", "KEGG:K10563", "RefSeq:WP_014894643.1", "SO:0001217", "UniParc:UPI00027B3AC2", "UniRef:UniRef100_UPI00027B3AC2", "UniRef:UniRef50_P55825", "UniRef:UniRef90_P55825" ], "nt": "ATGCCTGAACTTCCTGAAGTAACTACTGTTATTAATGAACTTAAAGAAACTGTTTTAAATAAACCTTTAGATCAAGTTCAAGTTAACCTAAGAAAGGTTTTGAAAAATATTGATCCTCAATTGCTGAATAAACAATTAAAAAATCAGTTTTTTACTGATATTAAGCGTAAGGGTAAATATATCATTTTTCTTTTAAGTAATGGTTTGTATTTAGTTTCGCATTTACGTATGGAAGGTAAATACTTTTTTGAAGAAAGAGGTAGTAAATTTAATCAAAAGCATGTTTTAGTAGAATTTCATTTTGATGATGGTAGTCAACTCAATTATCATGACACCAGACAATTTGGAACGTTCCATTTGTATGAAAAGTTAGAACAAGCAGCACAATTAAATAAACTTGCATTTGATCCTCTAGAAGCTGGTTTTGACTATAGGAAAATCTTCCAAAAAGCACAAAATTCAAAACGTAAAGTTAAAACTTTTATTTTAGACCAAACAGTGATTAGTGGAATTGGCAATATTTATGCAGATGAAATCTTATTTGCAAGCAAAATTAATCCTGAAACAATGGTTGATCAACTAACAATTAAAGAGATAGAGATTTTATGTAAAAATGCTACCAAAATTTTAGCTAAAGCAATAGTTATGAAAGGTACTACCACCAGCAGCTTTAGTTTTAAAAAAGATCATACTGGAGGCTATCAAAACTTTTTAAAAGTTCACACTAAAAAAGATCAACCTTGCTCAGTTTGTAACCAATTAATTGTTAAAAAGAAGATTAATGGAAGGGGGAGCTATTTTTGTTTAAACTGTCAAAAAATCACAACCAAAGTTTCTACAAAACTCAATCCATAA", "aa": "MPELPEVTTVINELKETVLNKPLDQVQVNLRKVLKNIDPQLLNKQLKNQFFTDIKRKGKYIIFLLSNGLYLVSHLRMEGKYFFEERGSKFNQKHVLVEFHFDDGSQLNYHDTRQFGTFHLYEKLEQAAQLNKLAFDPLEAGFDYRKIFQKAQNSKRKVKTFILDQTVISGIGNIYADEILFASKINPETMVDQLTIKEIEILCKNATKILAKAIVMKGTTTSSFSFKKDHTGGYQNFLKVHTKKDQPCSVCNQLIVKKKINGRGSYFCLNCQKITTKVSTKLNP", "aa_hexdigest": "c31acd6c30106555f2a2d01d6372d3e6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3AC2", "ncbi_nrp_id": "WP_014894643.1", "uniref100_id": "UniRef100_UPI00027B3AC2", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3AC2", "RefSeq:WP_014894643.1", "UniRef:UniRef100_UPI00027B3AC2" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3AC2", "uniref90_id": "UniRef90_P55825" }, "psc": { "uniref90_id": "UniRef90_P55825", "gene": "mutM", "product": "DNA-formamidopyrimidine glycosylase", "ec_ids": [ "3.2.2.23" ], "uniref50_id": "UniRef50_P55825", "cog_id": "COG0266", "cog_category": "L", "kegg_orthology_id": "K10563", "go_ids": [ "GO:0003684", "GO:0003906", "GO:0005737", "GO:0006281", "GO:0006284", "GO:0008270", "GO:0008534", "GO:0034039", "GO:0046872", "GO:0140078" ] }, "pscc": { "uniref50_id": "UniRef50_P55825", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P55825" ], "product": "Formamidopyrimidine-DNA glycosylase" }, "genes": [ "mutM" ], "id": "JPCNHPFPBM_600", "locus": "GCA000292505_00578" }, { "type": "cds", "sequence": "contig_1", "start": 320113, "stop": 320259, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTACCATTACTTTAGATGGGGCTATTATCTTTTATCAAAACGAAATCTTAAGTCAAACTTATTTAGATGATAGATTTATTGAAGGGATTAATAACATAGTAAAGCGCTTTCCTGAAGCTGCTTATAAACTTAACAGTGGTTGA", "aa": "MPTITLDGAIIFYQNEILSQTYLDDRFIEGINNIVKRFPEAAYKLNSG", "aa_hexdigest": "f20adc33eaa4cc4f8bdb9c3ebc48e9ec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5494.1902, "isoelectric_point": 4.593750190734863 }, "id": "JPCNHPFPBM_601", "locus": "GCA000292505_00579" }, { "type": "cds", "sequence": "contig_1", "start": 320437, "stop": 320634, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGCAAAAGGAAATAATTGAACTAATTAGTGTTAGTGATGTAAATGCCAAAATCTATGAGCACTCTATGTACATTATTAAAAATGGTGTTTCTAAAGCTAGTGCATTGCAAAGCCTTAACCAGTTTGCAATTCCAATAACAAAAGATAACACTATTGTTTGTGGGGATGGAGATAATGACATTGAAATGATGCAGTGA", "aa": "MQKEIIELISVSDVNAKIYEHSMYIIKNGVSKASALQSLNQFAIPITKDNTIVCGDGDNDIEMMQ", "aa_hexdigest": "d47c9668724ae7cabc2cd0138eac8d98", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 50, "aa_cov": 0.7692307692307693, "hmm_cov": 0.98, "evalue": 3.3020704364014056e-11, "score": 38.46260070800781, "start": 16, "stop": 65 } ], "seq_stats": { "molecular_weight": 7217.211100000002, "isoelectric_point": 4.5550428390502935 }, "id": "JPCNHPFPBM_602", "locus": "GCA000292505_00580" }, { "type": "cds", "sequence": "contig_1", "start": 320751, "stop": 321029, "strand": "+", "frame": 3, "gene": "coaE", "product": "dephospho-CoA kinase", "db_xrefs": [ "COG:COG0237", "COG:H", "EC:2.7.1.24", "GO:0004140", "GO:0005524", "GO:0005737", "GO:0015937", "KEGG:K00859", "SO:0001217", "UniRef:UniRef50_P47506", "UniRef:UniRef90_P47506" ], "nt": "ATGTTAATTGCAATCGTAGGTAAACCAGGTGTTGGTAAAACCAGTCTATTGCAATATCTCAAAGATAACTATCACTTTTCAGTTTTTTATGCAGATAGTTTTATCCATGAACAGTACCAAAAAAACAATCCAGGTTATCAATTAATCATGGATCATTTTGGCAAAGAGTTTGTCAATCAAACTGAAGTTGATCGTAAAAAACTAGCAAACTATGTTTTTAGTGATGATAAGTTAATCGAAAAACTTTCACTAGTAACAAAACCGCTGTTAATAGCGTGA", "aa": "MLIAIVGKPGVGKTSLLQYLKDNYHFSVFYADSFIHEQYQKNNPGYQLIMDHFGKEFVNQTEVDRKKLANYVFSDDKLIEKLSLVTKPLLIA", "aa_hexdigest": "451dec50d2b01e479ee19b450e831152", "start_type": "ATG", "rbs_motif": null, "genes": [ "coaE" ], "truncated": "3-prime", "pseudogene": { "start": 320751, "stop": 321332, "observations": { "mutation": [ 321027, 321099 ], "stop": [ 321027, 321099 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47506", "query_cov": 1.0, "subject_cov": 0.46464646464646464, "identity": 1.0, "score": 185.0, "evalue": 5.69e-58, "gene_start": 1, "gene_end": 92, "reference_start": 1, "reference_end": 92, "reference_sequence": "MLIAIVGKPGVGKTSLLQYLKDNYHFSVFYADSFIHEQYQKNNPGYQLIMDHFGKEFVNQTEVDRKKLANYVFSDDKLIEKLSLVTKPLLIAWIKSLKTQFQKKLALIEIAVMLNYWNEYRSLFDYVIKLERDDQLVNLALQQRNSHKKVKDLIKEPNCKIDTIFNNDSIATAALKLIKLLETFLERNKCRCDCCHIQ" }, "paralog": false, "identity": 0.9896373056994818, "score": 377.0, "evalue": 4.32e-136, "description": "Internal stop codon at 321027, 321099. Nonsense mutation around 321027, 321099.", "psc": { "uniref90_id": "UniRef90_P47506", "gene": "coaE", "product": "dephospho-CoA kinase", "ec_ids": [ "2.7.1.24" ], "uniref50_id": "UniRef50_P47506", "cog_id": "COG0237", "cog_category": "H", "kegg_orthology_id": "K00859", "go_ids": [ "GO:0004140", "GO:0005524", "GO:0005737", "GO:0015937" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47506", "EC:2.7.1.24", "UniRef:UniRef50_P47506", "COG:COG0237", "COG:H", "KEGG:K00859", "GO:0004140", "GO:0005524", "GO:0005737", "GO:0015937" ] }, "pscc": { "uniref50_id": "UniRef50_P47506", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47506" ], "product": "Dephospho-CoA kinase" } }, "id": "JPCNHPFPBM_603", "locus": "GCA000292505_00581" }, { "type": "cds", "sequence": "contig_1", "start": 321129, "stop": 321347, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTAAATTAGAAAGGGATGATCAGCTAGTTAACTTAGCTTTACAACAACGTAATAGTCATAAAAAAGTTAAGGATTTGATTAAAGAGCCTAATTGCAAAATAGATACAATTTTCAACAACGATTCGATTGCAACAGCTGCTTTAAAGCTAATTAAGTTGCTAGAAACTTTTTTAGAAAGAAATAAATGCCGTTGTGATTGTTGTCATATTCAGTAA", "aa": "MIKLERDDQLVNLALQQRNSHKKVKDLIKEPNCKIDTIFNNDSIATAALKLIKLLETFLERNKCRCDCCHIQ", "aa_hexdigest": "dff1722a4cb657f92f8c5d87688485c6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8396.792599999993, "isoelectric_point": 8.678105354309082 }, "id": "JPCNHPFPBM_604", "locus": "GCA000292505_00582" }, { "type": "cds", "sequence": "contig_1", "start": 321283, "stop": 322119, "strand": "-", "frame": 1, "gene": "cof", "product": "Putative phosphatase MG265", "db_xrefs": [ "COG:COG0561", "COG:HR", "EC:3.1.3.-", "GO:0000287", "GO:0005829", "GO:0016791", "KEGG:K21064", "RefSeq:WP_009885896.1", "SO:0001217", "UniParc:UPI0000139535", "UniRef:UniRef100_P47507", "UniRef:UniRef50_P75399", "UniRef:UniRef90_P47507" ], "nt": "ATGGAACTGAAAAACATTATTTTTGACCTTGATGGTACCTTGCTTTCAAGCAACCAAATTCCATTAGAACAAACAGTTGAGTTTTTAAAGGATTTACAGAAAAAAGGGATTAGAATCACTTTTGCTAGTGGTAGAAGCCATATTTTAATTAGAAACACAGCTACCTTTATTACACCAAATCTACCTGTAATTTCTTCCAATGGTGCACTTGTTTATGATTTTGCTAGTGAAAAACCAGTTCATATCAAACCTATTGATAATAAAGTAATACCTGCAATTATGCAAATGTTGTTGGAATTTCAAGAAACATTTTATTTCTATACAGATAAAAAGGTTTTTGCTTTTACACATGAGCTTGATTCAGCTAAAATTCTTTCAACTAGAAGTCAAATAGTAGGAATTGATCTCATTGAAAATAACTACATAGTTAACAAGTTTGAAAAAGCTTTGGATTTTGATTTTAAGCAACATACTATTACAAAGATCTTACTGGTAACTAAAAACAGAGAAAAAGTTCCTTTTCTAGCAAAACAACTAGATCAAATTCAAGATATTAACTATGTGAGTTCAATGACATTTGCTCTTGATATCATGCAAAAAGATGTTAATAAGGCTTATGGATTGAAAGTATTAGTTGATAATTATAATCTTGATCCTGAAAAGACTATGGTCTTTGGTGATGCTGATAATGATGTTGAAATATTTCAAAGTGTTAAATGGCCAGTTGCTTTGGTTAATGGCACTGATTTAGCTAAAAAAAATGCTAAGTTTATTACTGAATATGACAACAATCACAACGGCATTTATTTCTTTCTAAAAAAGTTTCTAGCAACTTAA", "aa": "MELKNIIFDLDGTLLSSNQIPLEQTVEFLKDLQKKGIRITFASGRSHILIRNTATFITPNLPVISSNGALVYDFASEKPVHIKPIDNKVIPAIMQMLLEFQETFYFYTDKKVFAFTHELDSAKILSTRSQIVGIDLIENNYIVNKFEKALDFDFKQHTITKILLVTKNREKVPFLAKQLDQIQDINYVSSMTFALDIMQKDVNKAYGLKVLVDNYNLDPEKTMVFGDADNDVEIFQSVKWPVALVNGTDLAKKNAKFITEYDNNHNGIYFFLKKFLAT", "aa_hexdigest": "a8175bd90a9b8fe365110442e827d3a1", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139535", "ncbi_nrp_id": "WP_009885896.1", "uniref100_id": "UniRef100_P47507", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139535", "RefSeq:WP_009885896.1", "UniRef:UniRef100_P47507" ] }, "ips": { "uniref100_id": "UniRef100_P47507", "uniref90_id": "UniRef90_P47507" }, "psc": { "uniref90_id": "UniRef90_P47507", "gene": "Cof", "product": "Putative phosphatase MG265", "ec_ids": [ "3.1.3.-" ], "uniref50_id": "UniRef50_P75399", "cog_id": "COG0561", "cog_category": "HR", "kegg_orthology_id": "K21064", "go_ids": [ "GO:0005829", "GO:0000287", "GO:0016791" ] }, "pscc": { "uniref50_id": "UniRef50_P75399", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75399" ], "product": "Putative phosphatase MPN_383" }, "genes": [ "cof" ], "id": "JPCNHPFPBM_605", "locus": "GCA000292505_00583" }, { "type": "cds", "sequence": "contig_1", "start": 322138, "stop": 322659, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08264.19" ], "nt": "TTGGATCGAGTTTATAACTTCTTTTTTAATCATGCTGTTGTTACTGATCAAGTTAGTCAAGAGACAACCTTTGCTTACAATTTGTTTTTAAAAAACAGTTATTGTCATCTTGACAAACATGAACTAAATCTGGTGATTAGTGAAATGATGATCTTTTTAAACTTTCTCTATAAAACCAAAAAAATTAGCTTAAATTATGCAAAGGGATTTTTAACAGTACTGTCGTTTTTTGCGCCCTTTCTTGCTGAAGAATTGAATGAAAAATGTGGACTTGAACCATTTGTTGTTAAACAAGCGATTTCTTTAGTTGATTATCAACTTTTTGAGACTGCTAAAACTAAGGTTATTCTTTCAATTAATGGCAAATTTAAAGCAGCTAAAGAATTTACTAAAGGTAGTTTAGAGATAGATGTTTTAGAATCATTTAAACAGGATAAAGAGATAAATGACATTCTCAACCAACCGATTGAGAGGGTAGTTTATGTTCAGGATCGAATTATTAATGTTCTTTTAAAAAAATAG", "aa": "MDRVYNFFFNHAVVTDQVSQETTFAYNLFLKNSYCHLDKHELNLVISEMMIFLNFLYKTKKISLNYAKGFLTVLSFFAPFLAEELNEKCGLEPFVVKQAISLVDYQLFETAKTKVILSINGKFKAAKEFTKGSLEIDVLESFKQDKEINDILNQPIERVVYVQDRIINVLLKK", "aa_hexdigest": "2c9758d3cb5b5ec32c70d3d00cd4f996", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08264.19", "name": "Anticodon_1", "length": 132, "aa_cov": 0.6011560693641619, "hmm_cov": 0.9924242424242424, "evalue": 5.483164632016121e-09, "score": 31.171886444091797, "start": 29, "stop": 132 } ], "seq_stats": { "molecular_weight": 20140.220100000002, "isoelectric_point": 6.833490562438965 }, "id": "JPCNHPFPBM_606", "locus": "GCA000292505_00584" }, { "type": "cds", "sequence": "contig_1", "start": 322693, "stop": 322896, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "TTGTTTGACAAGAAGCTAGTATCAACAAAAGAACCATTTCAAAAATTAATTAATCAGGGTATGGTGTTAGGTCCTGATGGTAAAAAGATGTCCAAATCCAAAGGTAATACCATTAACCCCACACCACTTGTTGATTCACATGGAGCAGATGCTTTAAGGTTGTACTTAATGTTTATGGGCCCAATTAGTGCTAGTTTAACTTGA", "aa": "MFDKKLVSTKEPFQKLINQGMVLGPDGKKMSKSKGNTINPTPLVDSHGADALRLYLMFMGPISASLT", "aa_hexdigest": "eb115c2118611b228a8c3c32605c2634", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 52, "aa_cov": 0.7761194029850746, "hmm_cov": 0.9807692307692307, "evalue": 1.949771903311517e-17, "score": 58.140174865722656, "start": 8, "stop": 59 } ], "seq_stats": { "molecular_weight": 7295.5466000000015, "isoelectric_point": 9.698381996154787 }, "id": "JPCNHPFPBM_607", "locus": "GCA000292505_00585" }, { "type": "cds", "sequence": "contig_1", "start": 323077, "stop": 323565, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28", "PFAM:PF13603.12" ], "nt": "TTGATCCCTGTCTATGTTGCTAACTATGTAATTGAAGGATATGGAACAGATGCTATTATGGGTGTTGGAGCACACAATGAAAATGATAACTTCTTCGCACGTAAACAAAAGTTGAAAATTATCAACGTCATTGATAAAAAAGAACGGCTGCAAAATTCATTTGCATATAACGGATTAACAACTAAAGAAGCACAAGTAGCTATTACTAATGAGTTAATTTCACAAAATAAAGCGAAATTAACAACTGTATATAAACTGCGTGATTGGATCTTCAGTAGACAGCGTTATTGGGGCGAACCTTTTCCAATTATTTTTGATGAAAATAACACTCCTCATTTGGTAGAACAACTCCCTGTTGAATTACCCTTACTTGAGAATTACAAACCAGATGGAAGTGGTAATTCTCCACTAATGAGAAATCAAGCTTGGGTAAACATAGTCAAAGATAACATCCATTACCAAAGGGAAACTAATACCATGCCCCAATGA", "aa": "MIPVYVANYVIEGYGTDAIMGVGAHNENDNFFARKQKLKIINVIDKKERLQNSFAYNGLTTKEAQVAITNELISQNKAKLTTVYKLRDWIFSRQRYWGEPFPIIFDENNTPHLVEQLPVELPLLENYKPDGSGNSPLMRNQAWVNIVKDNIHYQRETNTMPQ", "aa_hexdigest": "6d1f30fdb06f2ff605d0193935d50714", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 37, "aa_cov": 0.22839506172839505, "hmm_cov": 1.0, "evalue": 2.2761124900458792e-07, "score": 24.979543685913086, "start": 75, "stop": 111 }, { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 57, "aa_cov": 0.345679012345679, "hmm_cov": 0.9824561403508771, "evalue": 5.750437523110523e-18, "score": 60.13599395751953, "start": 2, "stop": 57 } ], "seq_stats": { "molecular_weight": 18758.094199999996, "isoelectric_point": 6.838776588439941 }, "id": "JPCNHPFPBM_608", "locus": "GCA000292505_00586" }, { "type": "cds", "sequence": "contig_1", "start": 324223, "stop": 324408, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGTTCCCCTATCCCTCAGCAGCAGGATTACATTTAGGACATGTTAGAGCTTATACTATCACTGATGTAATAAGTAGGTATTACAAAGCTAAAGGATTTAATGTGATCCATCCGATTGGTTTTGATGCTTTTGGTTTACCTGCTGAACAGTATGCTATTAACTCTAATCAAAACCCTGGCAGTTGA", "aa": "MFPYPSAAGLHLGHVRAYTITDVISRYYKAKGFNVIHPIGFDAFGLPAEQYAINSNQNPGS", "aa_hexdigest": "92924fc03ef03d109b9b91edf6dabae5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 47, "aa_cov": 0.7540983606557377, "hmm_cov": 1.0, "evalue": 8.982570098149723e-10, "score": 32.897457122802734, "start": 5, "stop": 50 } ], "seq_stats": { "molecular_weight": 6684.463900000002, "isoelectric_point": 8.122839164733886 }, "id": "JPCNHPFPBM_609", "locus": "GCA000292505_00587" }, { "type": "cds", "sequence": "contig_1", "start": 324517, "stop": 324639, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTCACTTTTTTATCCCTGTTGGCTTTGGGTTGTTATTTGGTTTGATTATTGCGATTATTGTTGACATACTCTATCGCTTAACTAAATATGCAATCAAGCGTTCTTATCAAAGCAATTAG", "aa": "MVHFFIPVGFGLLFGLIIAIIVDILYRLTKYAIKRSYQSN", "aa_hexdigest": "57dde39cf5d183e04b35dde3385cf6f5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4631.5682000000015, "isoelectric_point": 9.819775962829588 }, "id": "JPCNHPFPBM_610", "locus": "GCA000292505_00588" }, { "type": "cds", "sequence": "contig_1", "start": 324910, "stop": 325110, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTAACCGCAAAGTAGAGATTGATAACTTTACTAAAAATCAACTTTACTTTCAAAATTTACACAGGGTTTACACTGGATTTATGGAAGCGGTTTGTAATGATTTTGGGATTAATTACTGTATTATAGATGCAAAACTACCAATAGTAACTATTATTAAAATGATCCTTGAAAAATTAAAGTTACAAAAGTTAGATTGA", "aa": "MRNRKVEIDNFTKNQLYFQNLHRVYTGFMEAVCNDFGINYCIIDAKLPIVTIIKMILEKLKLQKLD", "aa_hexdigest": "91b11843a60bdb49a0903a8ab9ca84aa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7866.274499999997, "isoelectric_point": 9.031456565856931 }, "id": "JPCNHPFPBM_611", "locus": "GCA000292505_00589" }, { "type": "cds", "sequence": "contig_1", "start": 325207, "stop": 325584, "strand": "-", "frame": 1, "gene": "dck", "product": "putative protein MG268", "db_xrefs": [ "COG:COG1428", "COG:F", "GO:0005524", "GO:0005737", "GO:0019136", "SO:0001217", "UniRef:UniRef50_P75396", "UniRef:UniRef90_P47510" ], "nt": "ATGCAACTAAAAAAGCCCCATTTTCAACCAAATAAAATTGCTAATTGTATTGTGATCGGGGGAATGATTGCTTTAGGAAAAACCACCATTGCTAATACATTAGCTAACCACATTCAAGCTGCAAAAGTTGTTTGTGAATTGGAAACTAATGACCAGTTGGTTGAACTTTTACTAGCAAAGATGTATGAACGTAGTGATGAATTGCTCTATTCACCTTTGTTTCAGCTTTATTTTACGCTTAATCGCTTTGGTAAATACCAAACCAATTGCAACACTATCAATCCAACCATCTTTGATCGTTCTATCTTTGAAGACTGGTTGTTTGCTAAGCACAACATCATTCGTCCTGCAGTCTTTTCATACTATAACCAACTGTGA", "aa": "MQLKKPHFQPNKIANCIVIGGMIALGKTTIANTLANHIQAAKVVCELETNDQLVELLLAKMYERSDELLYSPLFQLYFTLNRFGKYQTNCNTINPTIFDRSIFEDWLFAKHNIIRPAVFSYYNQL", "aa_hexdigest": "8c876170f7e31d252912325ea40501d8", "start_type": "ATG", "rbs_motif": null, "genes": [ "dck" ], "truncated": "5-prime", "pseudogene": { "start": 324904, "stop": 325584, "observations": { "mutation": [ 324912, 325137, 325209 ], "stop": [ 324912, 325137, 325209 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47510", "query_cov": 1.0, "subject_cov": 0.5482456140350878, "identity": 0.992, "score": 256.0, "evalue": 5.99e-85, "gene_start": 1, "gene_end": 125, "reference_start": 1, "reference_end": 125, "reference_sequence": "MQLKKPHFQPNKIANCIVIGGMIALGKTTIANTLANHIQAAKVVCELETNDQLVELLLAKMYERSDELLYSPLFQLYFTLNRFGKYQNNCNTINPTIFDRSIFEDWLFAKHNIIRPAVFSYYNQLWNRLAKELVNKHGVPNLYVILDGDWKLFEKRLFMRNRKVEIDNFTKNQLYFQNLHRVYTGFMEAVCNDFGINYCIIDAKLPIVTIIKMILEKLKLQKLDWKFI" }, "paralog": false, "identity": 0.9823008849557522, "score": 447.0, "evalue": 8.78e-163, "description": "Internal stop codon at 324912, 325137, 325209. Nonsense mutation around 324912, 325137, 325209.", "psc": { "uniref90_id": "UniRef90_P47510", "gene": "Dck", "product": "Uncharacterized protein MG268", "uniref50_id": "UniRef50_P75396", "cog_id": "COG1428", "cog_category": "F", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0019136" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47510", "UniRef:UniRef50_P75396", "COG:COG1428", "COG:F", "GO:0005737", "GO:0005524", "GO:0019136" ] }, "pscc": { "uniref50_id": "UniRef50_P75396", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75396" ], "product": "Uncharacterized protein MG268 homolog" } }, "id": "JPCNHPFPBM_612", "locus": "GCA000292505_00590" }, { "type": "ncRNA", "class": null, "sequence": "contig_1", "start": 325620, "stop": 325715, "strand": "-", "gene": "Bacteria_small_SRP", "product": "Bacterial small signal recognition particle RNA", "score": 61.6, "evalue": 5.5e-08, "db_xrefs": [ "RFAM:RF00169", "SO:0000655" ], "nt": "ATTTAGAACCATCACATCATTAGAGTCGAATCATGTCAGGCCAGAAATGGAGCAGCATTAAGACTTGTCAGTGAGTGTGATGGTTTTTTATTAAAA", "id": "JPCNHPFPBM_42", "locus": "GCA000292505_00591" }, { "type": "cds", "sequence": "contig_1", "start": 325735, "stop": 326757, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MG269", "db_xrefs": [ "RefSeq:WP_014894012.1", "SO:0001217", "UniParc:UPI00027B3AC5", "UniRef:UniRef100_UPI00027B3AC5", "UniRef:UniRef50_Q49407", "UniRef:UniRef90_Q49407" ], "nt": "ATGAATAATTTTGATTACTATAGAGACTTTGATGACTTTCAAAGAAGAGAAACTATTAACTTCTTTTTAGCCAAGTTTCCCTTTGCTTCCAAAGCCCAGCTTGATGTTTTTTTAGAACAAGCAAAAACTGCTTATGTCAAACTACGAACAAGCAACCCAAAAAATGTTGATTGGAATCAGATGCTTGTTTTGTTATTAAAAAAGTTTGGTCCTGAAGCGAGAAGTGAAAAAGAACGTTTAAAAAGGGTCTTAAGTTTGCAAGAACAACTTAAGGTACGCTTGGAAGGAGAGATTAACCGTCAAGTTCAACAGAACAGCGAACTTTTCTCACAACTCAAACAAAGTGAAAGTGAAATCATCCAAATGCAGCAGCTGGTAGAAGCAAAAGAACACCAAATTGAAGCATTAAATAAACAGTTACATGCAATAAAAGAAGCCAATAAGAAACTAATTGAAGAACATGAAAGTATTAATGTAGAGGAATTGTTAAAGGAATATGAAGTGCAGTGTAATGAAGCAATTTATAAACGTGATCAACACATCCAAACCGTATTTGAAGACAAGTTAGCACTTAAAGATGGGGAAATTAGTGAAACCCAATCACTTTTAAAAACCGCTGAAAAAGAAAAGCAAGCATTAAAAAAAGCATATAAGTTAGTTGTAAATTCGCTTCAAAAACACCAAAAACTCACCAACGAAATAGAAATTGATTTCACCAAGCTTGATGAGATTATTGCAACCATTTTTGATGAAACTAAGAATCCAAAGACTGGCTTTACTAACTTCATTAAGCAGTTTGAAAAAACCAAAGCAAAACTAACAAAAAAGATAGCTGAAATTACTAAACTTGATCATTCAACGCCAACAAATTATCAACAAGAAACTCCAGCGTCACAACAGCAATTAGATCAAGAGAATGAACCGATCAAACCATCTAAAAAATCAAATTCAAGTTCATTGCCTAGAGGCACTACACAACCCAAATCCAATTCTATTAACAGGGTTAGTAAATTAATTGATTAA", "aa": "MNNFDYYRDFDDFQRRETINFFLAKFPFASKAQLDVFLEQAKTAYVKLRTSNPKNVDWNQMLVLLLKKFGPEARSEKERLKRVLSLQEQLKVRLEGEINRQVQQNSELFSQLKQSESEIIQMQQLVEAKEHQIEALNKQLHAIKEANKKLIEEHESINVEELLKEYEVQCNEAIYKRDQHIQTVFEDKLALKDGEISETQSLLKTAEKEKQALKKAYKLVVNSLQKHQKLTNEIEIDFTKLDEIIATIFDETKNPKTGFTNFIKQFEKTKAKLTKKIAEITKLDHSTPTNYQQETPASQQQLDQENEPIKPSKKSNSSSLPRGTTQPKSNSINRVSKLID", "aa_hexdigest": "78f48afeffa01f5df8485874462e015f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3AC5", "ncbi_nrp_id": "WP_014894012.1", "uniref100_id": "UniRef100_UPI00027B3AC5", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3AC5", "RefSeq:WP_014894012.1", "UniRef:UniRef100_UPI00027B3AC5" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3AC5", "uniref90_id": "UniRef90_Q49407" }, "psc": { "uniref90_id": "UniRef90_Q49407", "product": "Uncharacterized protein MG269", "uniref50_id": "UniRef50_Q49407" }, "pscc": { "uniref50_id": "UniRef50_Q49407", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49407" ], "product": "Uncharacterized protein MG269" }, "genes": [], "id": "JPCNHPFPBM_613", "locus": "GCA000292505_00592" }, { "type": "cds", "sequence": "contig_1", "start": 326750, "stop": 327136, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MG269-1", "db_xrefs": [ "RefSeq:WP_009885901.1", "SO:0001217", "UniParc:UPI0000557E21", "UniRef:UniRef100_Q49329", "UniRef:UniRef50_Q49329", "UniRef:UniRef90_Q49329" ], "nt": "ATGGATAGTTTTGTTCAAAAATACACAAATGGCTTCAAATCAATTTTAAACAAGGTTGAAAAAACTGATTTTGCAACTATTAAAAGTGAATTTCAATATAACCAAGCTAACTTGGAATGGGTTGAAAGTAAAGTTAGTGATCTTAACAACTATTTACTAGATCCTAACCAGTTTAGTGATGTAGTTAGTTTTAAAAAAATTGCTAATGAAAAGTTAGATTTGTTTGTTAAAAACCATGGCAATAAACTCCCGTTTTTTCTCTTTACTAGTTTTGTTTTAGCAATCTTTAGTTTTGTTAGTGTTTATGTTAGACACCATTATGATTTAGACTTTAATGATCCTGATGCTATCATTAGCTTCTTTAGAGAATTAGCTTTCCATGAATAA", "aa": "MDSFVQKYTNGFKSILNKVEKTDFATIKSEFQYNQANLEWVESKVSDLNNYLLDPNQFSDVVSFKKIANEKLDLFVKNHGNKLPFFLFTSFVLAIFSFVSVYVRHHYDLDFNDPDAIISFFRELAFHE", "aa_hexdigest": "1146b1a58ef06b100147b7ea7666ba20", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000557E21", "ncbi_nrp_id": "WP_009885901.1", "uniref100_id": "UniRef100_Q49329", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000557E21", "RefSeq:WP_009885901.1", "UniRef:UniRef100_Q49329" ] }, "ips": { "uniref100_id": "UniRef100_Q49329", "uniref90_id": "UniRef90_Q49329" }, "psc": { "uniref90_id": "UniRef90_Q49329", "product": "Uncharacterized protein MG269.1", "uniref50_id": "UniRef50_Q49329" }, "pscc": { "uniref50_id": "UniRef50_Q49329", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49329" ], "product": "Uncharacterized protein MG269.1" }, "genes": [], "id": "JPCNHPFPBM_614", "locus": "GCA000292505_00593" }, { "type": "cds", "sequence": "contig_1", "start": 327426, "stop": 327536, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAATTTTTTCACTGTTACTGAAAAAGCAGAAACAATTGTTTTAACTAAAGATGCACTAGCAAAGGTTGAAAAAAGAGCAAAAGAACACTTTCAATCATGGGAGTGA", "aa": "MINFFTVTEKAETIVLTKDALAKVEKRAKEHFQSWE", "aa_hexdigest": "cd5d4224ba381beb8927f39d7f8f2154", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4239.8462, "isoelectric_point": 6.556798362731934 }, "id": "JPCNHPFPBM_615", "locus": "GCA000292505_00594" }, { "type": "cds", "sequence": "contig_1", "start": 327561, "stop": 328082, "strand": "-", "frame": 2, "gene": null, "product": "biotin/lipoate A/B protein ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0037DCED81" ], "nt": "TTGCTAACTGAATTTAGAAAAAATGAGTTAATTAAGGTCATCTACTTTTGGCAGAACGCTAACACTATTGTGGTGGGAAGAAACCAAAATACTTATGCTGAGGTTAACTTAAAGGAGTTGGAAAGTGATAAGGTTAACTTGTTTAGACGTTTTTCAGGCGGGGGAGCGGTGTTTCATGACCTTGGTAACATCTGTTTTTCTATTATTTTGCCAAGAACAGGTAAAGTGATGGAAAATGCTTATGAACAAACTACAAGAAATGTGGTGAAGTTCTTAAATAGCTTAAATGTACCTGCTGTATTTCATGGTCGTAATGACCTTGAGATTGATAACAAGAAGTTTTCTGGGTTAGCTGAATATATCGCTAAAGACAGGTTATTAGTCCATGGAACATTATTGTTTGACACTGACTTTTCTAAGTTAGCAAAGTATTTAAATGTTGATAAGACCAAGATAGCAAGTAAGGGTGTTGACAGTGTTGCTAAGCGCGTTGTTAATGTAAAGGAGTATTTACCAAATTGA", "aa": "MLTEFRKNELIKVIYFWQNANTIVVGRNQNTYAEVNLKELESDKVNLFRRFSGGGAVFHDLGNICFSIILPRTGKVMENAYEQTTRNVVKFLNSLNVPAVFHGRNDLEIDNKKFSGLAEYIAKDRLLVHGTLLFDTDFSKLAKYLNVDKTKIASKGVDSVAKRVVNVKEYLPN", "aa_hexdigest": "71f04e521f93787e6e2c37597bf38afa", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0037DCED81", "query_cov": 0.8901734104046243, "subject_cov": 0.8461538461538461, "identity": 0.526, "score": 164.0, "evalue": 9.35e-49, "valid": false, "product": "biotin/lipoate A/B protein ligase family protein", "uniref50_id": "UniRef50_UPI0037DCED81" }, "pscc": { "uniref50_id": "UniRef50_UPI0037DCED81", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0037DCED81" ], "product": "biotin/lipoate A/B protein ligase family protein" }, "genes": [], "id": "JPCNHPFPBM_616", "locus": "GCA000292505_00595" }, { "type": "cds", "sequence": "contig_1", "start": 328159, "stop": 329172, "strand": "-", "frame": 1, "gene": "lpdA", "product": "dihydrolipoyl dehydrogenase", "db_xrefs": [ "COG:C", "COG:COG1249", "EC:1.8.1.4", "GO:0004148", "GO:0005737", "GO:0006090", "GO:0006103", "GO:0050660", "KEGG:K00382", "SO:0001217", "UniRef:UniRef50_P75393", "UniRef:UniRef90_P47513" ], "nt": "GTGCAAGTAAACAACACAACTTACACCACTAACAACATTATTGTTGCAACCGGATCAAGACCAAGATACTTAACTTTACCAGGGTTTGAAAAAGCACAACAAGCTGGGTTTATCATTGACTCAACCCAAGCTTTGGCTTTAGAGGGAGTACCTAAGAAGTTTGTTGTAGTTGGGGGAGGTGTGATTGGGGTTGAGTTTGCTTTTTTATTTGCTTCATTAGGGAGTGAAGTGACCATTATCCAAGGTGTTGATAGGATTTTGGAGGTTTGTGATAGTGATGTTTCTGAACTGATAAGTAAAACCTTAAAAAACAAAGGAGTTCAGATTATTACCAATGCTCATGTTGTTAGAGCTGAAAACAACCAACTGTTTTACACAGTTAATGGAGTTGAACAGTCTGTAATTGGTGATAAAATCTTAGTTTCTATAGGAAGAATTGCTAACACAGAGTGTTTAGATCAACTTGATTTAAAACGTGACCATAACAACAAAATTGTTTTAAATGAAAAACTACAAACATCAACTACAAACATCTATCTAATAGGTGATGTTAACACGCAAATGATGTTGGCACACTACGCTTACCAACAGGGCAGATATGCTGTTGATCAAATTTTGAACCAAAACCAGGTAAAGCCTGCTGAAAAAAACAAGTGTCCTGCTTGTATTTACACAAATCCTGAAGTTGCTTTTGTAGGTTATAGTGAGATGGAATTGCAAAAAGAAAAGATTGATTATGTCAAATCTTCTTTGCCATTTATTTATAGTGGTAAAGCAATTGCAGATCATGAAACCAATGGCTTTGTCAAGATGATGTTTAATCCTAAAACTGGTGCTATCTTAGGTGGATGTATTATTGCTAGCACTGCTAGTGATATTATCGCTGAGCTTGCTTTGGTGATGGAAAACAACCTCACTGTGTTTGATATTGCCAATTCTATCTCACCCCATCCTACCATGAATGAAATGGTAACTGATGTTTGTAAAAAAGCGATCTTTGATTACTTTAGTTAA", "aa": "MQVNNTTYTTNNIIVATGSRPRYLTLPGFEKAQQAGFIIDSTQALALEGVPKKFVVVGGGVIGVEFAFLFASLGSEVTIIQGVDRILEVCDSDVSELISKTLKNKGVQIITNAHVVRAENNQLFYTVNGVEQSVIGDKILVSIGRIANTECLDQLDLKRDHNNKIVLNEKLQTSTTNIYLIGDVNTQMMLAHYAYQQGRYAVDQILNQNQVKPAEKNKCPACIYTNPEVAFVGYSEMELQKEKIDYVKSSLPFIYSGKAIADHETNGFVKMMFNPKTGAILGGCIIASTASDIIAELALVMENNLTVFDIANSISPHPTMNEMVTDVCKKAIFDYFS", "aa_hexdigest": "80fec97fb6accac563db00b62595a552", "start_type": "GTG", "rbs_motif": null, "genes": [ "lpdA" ], "truncated": "3-prime", "pseudogene": { "start": 328159, "stop": 329472, "observations": { "mutation": [ 329298 ], "stop": [ 329298 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47513", "query_cov": 1.0, "subject_cov": 0.737417943107221, "identity": 0.997, "score": 655.0, "evalue": 2.01e-235, "gene_start": 1, "gene_end": 337, "reference_start": 121, "reference_end": 457, "reference_sequence": "MDYDLIILGAGPAGYIAAEYAGKHKLKTLVIEKQYFGGVCLNVGCIPTKTLLKRAKIIDYLVHAKDYGITINGQAKLDWKQLLKQKQEVVDKLVAGVKTIIKGAKVESIEGEATVIDKNKVQVNNTTYTTNNIIVATGSRPRYLTLPGFEKAQQAGFIIDSTQALALEGVPKKFVVVGGGVIGVEFAFLFASLGSEVTIIQGVDRILEVCDSDVSELISKTLKNKGVQIITNAHVVRAENNQLFYTVNGVEQSVIGDKILVSIGRIANTECLDQLDLKRDHNNKIVLNEKLQTSTTNIYLIGDVNTQMMLAHYAYQQGRYAVDQILNQNQVKPAEKNKCPACIYTNPEVAFVGYSEMELQKEKIDYVKSSLPFIYSGKAIADHETNGFVKMMFNPKTGAILGGCIIASTASDIIAELALVMENNLTVFDIANSISPHPTMNEMVTDVCKKAIFDYFS" }, "paralog": false, "identity": 0.9977116704805492, "score": 854.0, "evalue": 1.84e-316, "description": "Internal stop codon at 329298. Nonsense mutation around 329298.", "psc": { "uniref90_id": "UniRef90_P47513", "gene": "lpdA", "product": "dihydrolipoyl dehydrogenase", "ec_ids": [ "1.8.1.4" ], "uniref50_id": "UniRef50_P75393", "cog_id": "COG1249", "cog_category": "C", "kegg_orthology_id": "K00382", "go_ids": [ "GO:0004148", "GO:0005737", "GO:0006090", "GO:0006103", "GO:0050660" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47513", "EC:1.8.1.4", "UniRef:UniRef50_P75393", "COG:COG1249", "COG:C", "KEGG:K00382", "GO:0004148", "GO:0005737", "GO:0006090", "GO:0006103", "GO:0050660" ] }, "pscc": { "uniref50_id": "UniRef50_P75393", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75393" ], "product": "Dihydrolipoyl dehydrogenase" } }, "id": "JPCNHPFPBM_617", "locus": "GCA000292505_00596" }, { "type": "cds", "sequence": "contig_1", "start": 329296, "stop": 329532, "strand": "-", "frame": 1, "gene": null, "product": "FAD-dependent oxidoreductase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X1ZRV2" ], "nt": "ATGGATTATGATCTAATTATTTTGGGTGCTGGCCCTGCTGGTTATATTGCTGCGGAGTATGCTGGCAAACATAAACTTAAAACCCTAGTGATTGAAAAGCAATACTTTGGTGGGGTGTGTTTAAATGTTGGGTGTATCCCAACTAAAACGTTGTTAAAAAGAGCAAAGATTATTGATTATTTAGTTCATGCCAAAGATTATGGTATCACTATTAATGGTCAAGCTAAACTTGATTGA", "aa": "MDYDLIILGAGPAGYIAAEYAGKHKLKTLVIEKQYFGGVCLNVGCIPTKTLLKRAKIIDYLVHAKDYGITINGQAKLD", "aa_hexdigest": "8b23540e0848f0b7478a32e15e6e2edb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0010C3BE0E", "query_cov": 0.9358974358974359, "subject_cov": 0.9358974358974359, "identity": 0.562, "score": 96.3, "evalue": 1.25e-24, "valid": false, "product": "FAD-dependent oxidoreductase", "uniref50_id": "UniRef50_A0A7X1ZRV2" }, "pscc": { "uniref50_id": "UniRef50_A0A7X1ZRV2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X1ZRV2" ], "product": "FAD-dependent oxidoreductase" }, "genes": [], "id": "JPCNHPFPBM_618", "locus": "GCA000292505_00597" }, { "type": "cds", "sequence": "contig_1", "start": 329536, "stop": 330690, "strand": "-", "frame": 1, "gene": "pdhC", "product": "Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex", "db_xrefs": [ "COG:C", "COG:COG0508", "EC:2.3.1.12", "GO:0004742", "GO:0005737", "GO:0016407", "GO:0031405", "RefSeq:WP_014894645.1", "SO:0001217", "UniParc:UPI00027B3AC7", "UniRef:UniRef100_UPI00027B3AC7", "UniRef:UniRef50_P47514", "UniRef:UniRef90_P47514" ], "nt": "ATGGCAAATGAGTTTAAATTCACTGATGTTGGTGAGGGTTTACATGAAGGAAAAGTAACTGAAATCTTAAAACAAGTTGGTGATCAGATCAAGATAGATGAAGCTTTATTTGTTGTTGAAACTGATAAAGTTACAACCGAATTACCTTCTCCTTTTGCAGGTACAATTAGCGCTATTAATGTTAAAGTTGGTGATGTTGTTAGCATTGGTCAGGTGATGGCAGTTATTGGTGAAAAGACTAGTACACCAATTGTTGAACCAAAACCTCAACCAACTGAAGAAGTAACTAAGGTAAAAGAAGCGGGGGCTTCAGTAGTAGGGGAAATTAAGGTTTCTGATAACCTCTTTCCTATCTTTGGAGTAAAACCTCATGCAACTCCAGCTGTTAAAGACACTAAAGTTGCAAGTAGTACTAACATTACTGTAGAAACAACCCAAAAACCAGAAAGTAAAACTGAACAGAAAACCATTGCTATCTCAACAATGCGCAAAGCGATTGCAGAAGCAATGACAAAGTCGCACGCAATTATCCCAACCACTGTATTAACTTTTTATGTTAATGCAACCAAGTTAAAACAATATCGTGAAAGTGTTAATGGTTATGCTTTAAGTAAGTATTCCATGAAAATTTCTTACTTTGCTTTCTTTGTTAAAGCAATTGTTAATGCGCTTAAGAAGTTCCCTGTTTTTAACGCTAGTTATGATCCTGATCAAAACGAAATTGTTTTAAATGATGACATTAATGTAGGAATTGCTGTTGATACTGAAGAAGGTTTAATTGTCCCTAACATTAAGCAAGCCCAAACCAAATCTGTGGTTGAAATTGCCCAAGCAATTGTTGATTTAGCTAACAAAGCTAGAACAAAAAAGATTAAGTTGACTGATTTGAATAAAGGTACTATTTCAGTTACTAACTTCGGTTCATTAGGAGCAGCTGTAGGTACACCTATTATTAAGTACCCTGAGATGTGTATTGTTGCTACTGGTAATTTAGAAGAACGCATTGTTAAAGTGGAAAATGGAATTGCAGTTCATACCATCTTACCTTTAACAATAGCTGCAGACCACCGCTGGGTTGATGGGGCGGATGTTGGTAGGTTTGGTAAGGAGATTGCAAAACAAATTGAGGAATTAATTGATCTTACAGTAGCTTAA", "aa": "MANEFKFTDVGEGLHEGKVTEILKQVGDQIKIDEALFVVETDKVTTELPSPFAGTISAINVKVGDVVSIGQVMAVIGEKTSTPIVEPKPQPTEEVTKVKEAGASVVGEIKVSDNLFPIFGVKPHATPAVKDTKVASSTNITVETTQKPESKTEQKTIAISTMRKAIAEAMTKSHAIIPTTVLTFYVNATKLKQYRESVNGYALSKYSMKISYFAFFVKAIVNALKKFPVFNASYDPDQNEIVLNDDINVGIAVDTEEGLIVPNIKQAQTKSVVEIAQAIVDLANKARTKKIKLTDLNKGTISVTNFGSLGAAVGTPIIKYPEMCIVATGNLEERIVKVENGIAVHTILPLTIAADHRWVDGADVGRFGKEIAKQIEELIDLTVA", "aa_hexdigest": "2f92a205694629166ad68be10b330145", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3AC7", "ncbi_nrp_id": "WP_014894645.1", "uniref100_id": "UniRef100_UPI00027B3AC7", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3AC7", "RefSeq:WP_014894645.1", "UniRef:UniRef100_UPI00027B3AC7" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3AC7", "uniref90_id": "UniRef90_P47514" }, "psc": { "uniref90_id": "UniRef90_P47514", "gene": "pdhC", "product": "Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex", "ec_ids": [ "2.3.1.12" ], "uniref50_id": "UniRef50_P47514", "cog_id": "COG0508", "cog_category": "C", "go_ids": [ "GO:0005737", "GO:0016407", "GO:0004742", "GO:0031405" ] }, "pscc": { "uniref50_id": "UniRef50_P47514", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47514" ], "product": "Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex" }, "genes": [ "pdhC" ], "id": "JPCNHPFPBM_619", "locus": "GCA000292505_00598" }, { "type": "cds", "sequence": "contig_1", "start": 330941, "stop": 331165, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02780.25" ], "nt": "GTGAAGAAAACAGGAAGACTACTTGTAGTGACTGAAGCGGTGAAAAGTTTCACTACAAGTGCAGAGATTATCACTTCAGTAACTGAAGAACTATTCACTTATCTCAAAAAAGCCCCACAACGGGTAACTGGGTTTGATATTGTTGTGCCTTTAGCTAGAGGTGAAAAATACCAGTTTGAAATTAATGCACGGGTTATTGATGCAGTTAATCAACTTTTAAAATAA", "aa": "MKKTGRLLVVTEAVKSFTTSAEIITSVTEELFTYLKKAPQRVTGFDIVVPLARGEKYQFEINARVIDAVNQLLK", "aa_hexdigest": "f5a9a38b26064ae10e52b945f1686ad8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02780.25", "name": "Transketolase_C", "length": 57, "aa_cov": 0.7702702702702703, "hmm_cov": 1.0, "evalue": 5.432473104409802e-13, "score": 44.06863021850586, "start": 1, "stop": 57 } ], "seq_stats": { "molecular_weight": 8315.6384, "isoelectric_point": 9.453788948059085 }, "id": "JPCNHPFPBM_620", "locus": "GCA000292505_00599" }, { "type": "cds", "sequence": "contig_1", "start": 331190, "stop": 331921, "strand": "-", "frame": 3, "gene": "pdhB", "product": "Pyruvate dehydrogenase E1 component subunit beta", "db_xrefs": [ "COG:C", "COG:COG0022", "EC:1.2.4.1", "GO:0004739", "GO:0007584", "GO:0009083", "GO:0140032", "SO:0001217", "UniRef:UniRef50_P75391", "UniRef:UniRef90_P47515" ], "nt": "ATGTCAAAAATCCAAGTAAATAACATTGAAGCGTTAAACAACGCAATGGATCTTGCACTGGAAAGAGATCAAAACGTTGTACTCTATGGCCAGGACGCTGGTTTTGAAGGGGGTGTATTCCGTGCAACTAAAGGTTTACAACAAAAGTATGGAAGTGAAAGGGTATGGGATTGTCCTATAGCAGAAAACTCTATGGCCGGTATTGGGGTTGGGGCTGCTATAGGTGGTCTTAAACCTATTGTAGAGATCCAGTTTTCAGGCTTTTCATTCCCAGCTATGTTTCAAATCTTTGTCCATGCTGCTAGGATTAGAAACCGTTCTCGTGGTGTATATACCGCTCCACTAGTAGTGAGGATGCCAATGGGTGGGGGGATTAAAGCATTGGAACACCACAGTGAAACATTGGAAGCAATTTATGCACAGATTGCTGGGCTTAAAACAGTGATGCCATCAAATCCTTATGATACCAAAGGACTTTTTCTAGCTGCTATTGAATCACCTGATCCTGTTATCTTTTTTGAACCAAAGAAGCTTTATCGTGCTTTTCGTCAGGAGATTCCTAGTGATTATTACACTGTCCCTATTGGTGAAGCCAACTTGATTAGTGAAGGTAGTGAACTTACAATAGTTAGCTATGGTCCTACAATGTTTGATTTAATTAACTTAGTTTACAGCGGGGAATTGAAAGATAAGGGAATTGAGTTAATTGACTTGCGTACTATCTCCCCTTGA", "aa": "MSKIQVNNIEALNNAMDLALERDQNVVLYGQDAGFEGGVFRATKGLQQKYGSERVWDCPIAENSMAGIGVGAAIGGLKPIVEIQFSGFSFPAMFQIFVHAARIRNRSRGVYTAPLVVRMPMGGGIKALEHHSETLEAIYAQIAGLKTVMPSNPYDTKGLFLAAIESPDPVIFFEPKKLYRAFRQEIPSDYYTVPIGEANLISEGSELTIVSYGPTMFDLINLVYSGELKDKGIELIDLRTISP", "aa_hexdigest": "7647151f52bb756121fcb1af4f6e51f9", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FDA3C09", "query_cov": 0.934156378600823, "subject_cov": 0.9700854700854701, "identity": 0.568, "score": 270.0, "evalue": 2.56e-88, "valid": false, "product": "transketolase C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A537VMS1" }, "genes": [ "pdhB" ], "truncated": "5-prime", "pseudogene": { "start": 330941, "stop": 331921, "observations": { "mutation": [ 331192 ], "stop": [ 331192 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47515", "query_cov": 1.0, "subject_cov": 0.745398773006135, "identity": 1.0, "score": 480.0, "evalue": 5.35e-170, "gene_start": 1, "gene_end": 243, "reference_start": 1, "reference_end": 243, "reference_sequence": "MSKIQVNNIEALNNAMDLALERDQNVVLYGQDAGFEGGVFRATKGLQQKYGSERVWDCPIAENSMAGIGVGAAIGGLKPIVEIQFSGFSFPAMFQIFVHAARIRNRSRGVYTAPLVVRMPMGGGIKALEHHSETLEAIYAQIAGLKTVMPSNPYDTKGLFLAAIESPDPVIFFEPKKLYRAFRQEIPSDYYTVPIGEANLISEGSELTIVSYGPTMFDLINLVYSGELKDKGIELIDLRTISPWDKQTVFNSVKKTGRLLVVTEAVKSFTTSAEIITSVTEELFTYLKKAPQRVTGFDIVVPLARGEKYQFEINARVIDAVNQLLK" }, "paralog": false, "identity": 0.9969325153374233, "score": 635.0, "evalue": 1.07e-233, "description": "Internal stop codon at 331192. Nonsense mutation around 331192.", "psc": { "uniref90_id": "UniRef90_P47515", "gene": "pdhB", "product": "Pyruvate dehydrogenase E1 component subunit beta", "ec_ids": [ "1.2.4.1" ], "uniref50_id": "UniRef50_P75391", "cog_id": "COG0022", "cog_category": "C", "go_ids": [ "GO:0140032", "GO:0004739", "GO:0009083", "GO:0007584" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47515", "EC:1.2.4.1", "UniRef:UniRef50_P75391", "COG:COG0022", "COG:C", "GO:0140032", "GO:0004739", "GO:0009083", "GO:0007584" ] }, "pscc": { "uniref50_id": "UniRef50_P75391", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75391" ], "product": "Pyruvate dehydrogenase E1 component subunit beta" } }, "id": "JPCNHPFPBM_621", "locus": "GCA000292505_00600" }, { "type": "cds", "sequence": "contig_1", "start": 331939, "stop": 332310, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00676.26" ], "nt": "ATGCAAGATACTGCTAATTACGCTAGAGGTGGTAATGGACCAGTCTTAATTGAGTTCTTCAGCTACCGGCAAGGTCCTCACACCACTTCAGATGATCCTTCTATCTACAGAACCAAACAAGAAGAGGAGGAGGGAATGAAGAGTGATCCAGTGAAGCGATTGCGAAACTTCTTGTTTGATAGATCAATTCTTAACCAAGCTCAAGAAGAAGAGATGTTCAGCAAAATTGAACAGGAAATCCAAGCTGCTTATGAAAAGATGGTACTAGATACCCCTGTATCAGTAGATGAGGTGTTTGATTACAACTACCAAGAATTAACCCCTGAACTAGTTGAACAGAAACAGATTGCAAAAAAATACTTTAAAGACTAA", "aa": "MQDTANYARGGNGPVLIEFFSYRQGPHTTSDDPSIYRTKQEEEEGMKSDPVKRLRNFLFDRSILNQAQEEEMFSKIEQEIQAAYEKMVLDTPVSVDEVFDYNYQELTPELVEQKQIAKKYFKD", "aa_hexdigest": "fab84f61476ded95ef8246d2cf0b72f1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00676.26", "name": "E1_dh", "length": 83, "aa_cov": 0.6666666666666666, "hmm_cov": 1.0, "evalue": 2.480962030172872e-23, "score": 77.55926513671875, "start": 5, "stop": 86 } ], "seq_stats": { "molecular_weight": 14442.879499999994, "isoelectric_point": 4.650248146057129 }, "id": "JPCNHPFPBM_622", "locus": "GCA000292505_00601" }, { "type": "cds", "sequence": "contig_1", "start": 332464, "stop": 332574, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00676.26" ], "nt": "ATGTTGCACTATAAAAAGCAACCTAATGTTGCTGTTACTATGATTGGTGATGGAGGTACAGCTGAAGGGGAATTTTATGAAGCGATGAACATTGCAAGCATCCACAAGTGA", "aa": "MLHYKKQPNVAVTMIGDGGTAEGEFYEAMNIASIHK", "aa_hexdigest": "210f36023b8fa0de2f1e35de77ddb3a1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00676.26", "name": "E1_dh", "length": 35, "aa_cov": 0.9722222222222222, "hmm_cov": 1.0, "evalue": 2.467318603810583e-10, "score": 34.88288497924805, "start": 2, "stop": 36 } ], "seq_stats": { "molecular_weight": 3952.4923, "isoelectric_point": 5.9887495040893555 }, "id": "JPCNHPFPBM_623", "locus": "GCA000292505_00602" }, { "type": "cds", "sequence": "contig_1", "start": 332677, "stop": 332829, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00676.26" ], "nt": "ATGTTAAACTTCGCTCCTAATTTGGGAGAGGAAGCTTTACAGGTTGGAATGGGATTAGGTTTAAATGAAAATGATTGGGTTTGTCCTACGTTTCGTAGTGGGGCTTTAATGTTGTATCGTGGGGTAAAACCAGAACAACTTTTACTCTACTGA", "aa": "MLNFAPNLGEEALQVGMGLGLNENDWVCPTFRSGALMLYRGVKPEQLLLY", "aa_hexdigest": "ffc2ecc1afcb252a67b29e8a824f6f8d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00676.26", "name": "E1_dh", "length": 45, "aa_cov": 0.9, "hmm_cov": 1.0, "evalue": 1.2982833299953834e-08, "score": 29.231674194335938, "start": 5, "stop": 49 } ], "seq_stats": { "molecular_weight": 5570.4433, "isoelectric_point": 4.584314918518066 }, "id": "JPCNHPFPBM_624", "locus": "GCA000292505_00603" }, { "type": "cds", "sequence": "contig_1", "start": 332845, "stop": 333015, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAATCTTGATTAAAAATAAAGTTCCAACTACCCTTTATCAGGTTTATGATAATGAGGGTAAATTAATTGATCCTAACCACAAAATTACCCTAACTGATGAACAGTTAAAACACGCTTATTACTTAATGAACTTGAGTAGAATGATGGACAAAAAGATGTTAGTTTGA", "aa": "MAILIKNKVPTTLYQVYDNEGKLIDPNHKITLTDEQLKHAYYLMNLSRMMDKKMLV", "aa_hexdigest": "397073f2a778dd24fee48785947303db", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6625.819500000001, "isoelectric_point": 9.00508899688721 }, "id": "JPCNHPFPBM_625", "locus": "GCA000292505_00604" }, { "type": "cds", "sequence": "contig_1", "start": 333043, "stop": 334125, "strand": "-", "frame": 1, "gene": "nox", "product": "NADH oxidase", "db_xrefs": [ "COG:COG0446", "COG:I", "EC:1.6.3.4", "GO:0008137", "SO:0001217", "UniRef:UniRef50_Q49408", "UniRef:UniRef90_Q49408" ], "nt": "ATGAATGTTGAAAACAAGGTGACACACAAGCCTTTGGAGTTTAACTACACTGACAAATATTGTGGTAATGTTAAGAACTTAATTAGCTGTAAGTTATACCAACATGCACTTACCTTAATCGATAGTTTTCGTAAAGATAAAACCATTAAATCAGTTGCTATTGTTGGTTCTGGTTACATTGGCTTGGAACTTGCTGAAGCAGCTTGGTTATGCAAAAAGCAAGTAACAGTAATTGACTTACTTGATAAGCCTGCTGGTAATAACTTTGATCATGAGTTTACTGATGAACTTAAAAAAGTGATGCAAAAAGATGGGTTAAAACTAATGATGGGTTGCAGTGTTAAGGGCTTTGTTGTTGATAGTACAAACAACGTTGTCAAAGGAGTTGAAACTGATAAGGGAATAGTAAATGCAGACCTTGTGATCCAATCAATTGGGTTTAGACCTAGCACTAAGTTTGTTCCTAAAGATCAAAACTTTGAGTTTATTCACAACGGTTCAATTAAAGTTAATGAATTTCTCCAAGCACTAAATCATAAGGATGTTTATGTCATTGGGGGTTGTGCTGCTATTTACAATGCTGCTAGTGAACAGTATGAAAACATCGATCTTGCTACCAATGCAGTAAAGAGTGGATTAGTAGCTGCGATGCATATCATTGGTAGTAACCAAGTTAAACTCCAATCTATCGTTGGCACCAATGCACTCCATATCTTTGGTTTAAATTTAGCAGCATGTGGATTAACTGAACAGCGTGCTAAGAAGTTAGGTTTTGATGTTGGCATATCAGTTGTTGATGACAATGATCGTCCTGAGTTTATGGGCAGTTATGACAAGGTGCGTTTTAAACTTGTATATGACAAGAAAACCCTAAGAATTTTAGGAGCACAACTCCTTTCTTGGAATACCAATCACAGTGAGATTATTTTCTATATTGCACTTGCAATCCAAAAGCAGATGTTACTAACTGAACTGGGTTTAGTGGATGTTTATTTTCTTCCACATTACAACAAACCGTTTAACTTTGTGTTAGCAACTGTTTTACAAGCACTTGGTTTTAGCTATTACATTCCTAAAAAATAG", "aa": "MNVENKVTHKPLEFNYTDKYCGNVKNLISCKLYQHALTLIDSFRKDKTIKSVAIVGSGYIGLELAEAAWLCKKQVTVIDLLDKPAGNNFDHEFTDELKKVMQKDGLKLMMGCSVKGFVVDSTNNVVKGVETDKGIVNADLVIQSIGFRPSTKFVPKDQNFEFIHNGSIKVNEFLQALNHKDVYVIGGCAAIYNAASEQYENIDLATNAVKSGLVAAMHIIGSNQVKLQSIVGTNALHIFGLNLAACGLTEQRAKKLGFDVGISVVDDNDRPEFMGSYDKVRFKLVYDKKTLRILGAQLLSWNTNHSEIIFYIALAIQKQMLLTELGLVDVYFLPHYNKPFNFVLATVLQALGFSYYIPKK", "aa_hexdigest": "e74610d8cebdc3040e2c5c5724d63628", "start_type": "ATG", "rbs_motif": null, "genes": [ "nox" ], "truncated": "3-prime", "pseudogene": { "start": 333043, "stop": 334425, "observations": { "mutation": [ 334137 ], "stop": [ 334137 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49408", "query_cov": 1.0, "subject_cov": 0.7531380753138075, "identity": 0.997, "score": 714.0, "evalue": 3.88e-258, "gene_start": 1, "gene_end": 360, "reference_start": 119, "reference_end": 478, "reference_sequence": "MKKVIVIGINHAGTSFIRTLLSKSKDFKVNAYDRNTNISFLGCGIALAVSGVVKNTDDLFYSNPEELKQMGANIFMSHDVTNIDLIKKQVTVRDLTSNKEFTDQFDQLVIASGAWPICMNVENKVTHKPLEFNYTDKYCGNVKNLISCKLYQHALTLIDSFRKDKTIKSVAIVGSGYIGLELAEAAWLCKKQVTVIDLLDKPAGNNFDHEFTDELEKVMQKDGLKLMMGCSVKGFVVDSTNNVVKGVETDKGIVNADLVIQSIGFRPSTKFVPKDQNFEFIHNGSIKVNEFLQALNHKDVYVIGGCAAIYNAASEQYENIDLATNAVKSGLVAAMHIIGSNQVKLQSIVGTNALHIFGLNLAACGLTEQRAKKLGFDVGISVVDDNDRPEFMGSYDKVRFKLVYDKKTLRILGAQLLSWNTNHSEIIFYIALAIQKQMLLTELGLVDVYFLPHYNKPFNFVLATVLQALGFSYYIPKK" }, "paralog": false, "identity": 0.9934782608695653, "score": 909.0, "evalue": 0.0, "description": "Internal stop codon at 334137. Nonsense mutation around 334137.", "psc": { "uniref90_id": "UniRef90_Q49408", "gene": "nox", "product": "NADH oxidase", "ec_ids": [ "1.6.3.4" ], "uniref50_id": "UniRef50_Q49408", "cog_id": "COG0446", "cog_category": "I", "go_ids": [ "GO:0008137" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49408", "EC:1.6.3.4", "UniRef:UniRef50_Q49408", "COG:COG0446", "COG:I", "GO:0008137" ] }, "pscc": { "uniref50_id": "UniRef50_Q49408", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49408" ], "product": "NADH oxidase" } }, "id": "JPCNHPFPBM_626", "locus": "GCA000292505_00605" }, { "type": "cds", "sequence": "contig_1", "start": 334135, "stop": 334479, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07992.20" ], "nt": "ATGAAAAAAGTGATTGTGATTGGAATAAATCACGCTGGTACTAGTTTTATTAGAACTTTACTTTCAAAAAGTAAGGACTTTAAGGTTAACGCTTATGATAGAAACACAAACATCTCGTTTCTGGGGTGTGGAATTGCACTTGCTGTTAGTGGTGTTGTTAAAAACACTGATGATCTTTTCTATTCCAACCCTAAGGAGTTGAAACAGATGGGCGCTAACATCTTTATGAGTCATGATGTTACTAACATTGATCTAATCAAAAAACAGGTAACAGTTAGAGATTTAACATCAAATAAAGAGTTCACTGATCAGTTTGATCAACTAGTAATCGCTTCAGGAGCATGA", "aa": "MKKVIVIGINHAGTSFIRTLLSKSKDFKVNAYDRNTNISFLGCGIALAVSGVVKNTDDLFYSNPKELKQMGANIFMSHDVTNIDLIKKQVTVRDLTSNKEFTDQFDQLVIASGA", "aa_hexdigest": "a83e6359e8a23136933733979a36d6a2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07992.20", "name": "Pyr_redox_2", "length": 115, "aa_cov": 0.9912280701754386, "hmm_cov": 0.9478260869565217, "evalue": 1.1067371013548913e-17, "score": 59.45166778564453, "start": 2, "stop": 114 } ], "seq_stats": { "molecular_weight": 12585.34589999999, "isoelectric_point": 9.09856815338135 }, "id": "JPCNHPFPBM_627", "locus": "GCA000292505_00606" }, { "type": "cds", "sequence": "contig_1", "start": 334562, "stop": 335104, "strand": "-", "frame": 3, "gene": "apt", "product": "adenine phosphoribosyltransferase", "db_xrefs": [ "COG:COG0503", "COG:F", "EC:2.4.2.7", "GO:0002055", "GO:0003999", "GO:0005737", "GO:0006166", "GO:0006168", "GO:0016208", "GO:0044209", "KEGG:K00759", "RefSeq:WP_010869402.1", "SO:0001217", "UniParc:UPI0000125CDE", "UniRef:UniRef100_P47518", "UniRef:UniRef50_P75388", "UniRef:UniRef90_P47518" ], "nt": "ATGGATCAAAACTTTAAGTTGCTTGATCAAGCCATCAAGCGCTTTGAAAATTTTCCCAACCAAGGTACATTGTTTTATGACATTACCCCAGTATTTTCCAATCCCCAACTATTTAATTTTGTGCTAACCCAAATGGCACAGTTTATTAAAGCTATTAATGCAGAAGCGATAGTATGTCCTGAAGCGAGGGGTTTTATCTTTGGGGGAGCATTAGCTTCTAAAACCCAACTCCCGTTAGTATTGGTTAGAAAAGCCAATAAACTCCCAGGGCAATTAATTAGTGCTAGCTATGATTTGGAGTACAGAAAACATGCTGTATTGGAGATGTCAACCACTTCATTAATCCAAGCTAATAATGCTAAAAGGTGTGTTATTGTTGATGATGTACTTGCCACTGCTGGAACAGTTGCTGCTATTGACCAATTACTTAAACAGTTAAATGGTGAAACTGTGGGATATTGTTTTTTAATTGAGCTGAAAAAACTCAATGGTAAAGCTAAGTTACAACCAAATGTGGTTAGCAAGATTTTATTACATTACTAG", "aa": "MDQNFKLLDQAIKRFENFPNQGTLFYDITPVFSNPQLFNFVLTQMAQFIKAINAEAIVCPEARGFIFGGALASKTQLPLVLVRKANKLPGQLISASYDLEYRKHAVLEMSTTSLIQANNAKRCVIVDDVLATAGTVAAIDQLLKQLNGETVGYCFLIELKKLNGKAKLQPNVVSKILLHY", "aa_hexdigest": "2101c24b54a703e57741de4f79e240c8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000125CDE", "ncbi_nrp_id": "WP_010869402.1", "uniref100_id": "UniRef100_P47518", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000125CDE", "RefSeq:WP_010869402.1", "UniRef:UniRef100_P47518" ] }, "ips": { "uniref100_id": "UniRef100_P47518", "uniref90_id": "UniRef90_P47518" }, "psc": { "uniref90_id": "UniRef90_P47518", "gene": "apt", "product": "adenine phosphoribosyltransferase", "ec_ids": [ "2.4.2.7" ], "uniref50_id": "UniRef50_P75388", "cog_id": "COG0503", "cog_category": "F", "kegg_orthology_id": "K00759", "go_ids": [ "GO:0002055", "GO:0003999", "GO:0005737", "GO:0006166", "GO:0006168", "GO:0016208", "GO:0044209" ] }, "pscc": { "uniref50_id": "UniRef50_P75388", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75388" ], "product": "Adenine phosphoribosyltransferase" }, "genes": [ "apt" ], "id": "JPCNHPFPBM_628", "locus": "GCA000292505_00607" }, { "type": "cds", "sequence": "contig_1", "start": 335232, "stop": 335918, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACACAGACTTTTTTATAACCAAAAACCAACATGATTTCTTTTTGAAAAACAACTGGAAGTTTTTGTTTGTATGGCTAATCTTGTTAGCTATAGGAGTTGTAATGCTAGTTTTATATCTTGTTCAACCTAACCTGTTAGGAGAATTTTTAGCAGCAGATGTTGAATCAAGTAACGGGATTATTGCTGGGATAGGAATAGTTAGTTTACTGTACTTAGCTTATTGTTTGATTAGATACGGTGTTGCTTATTGTTTAAGTTATTTGGTTAGCTTTATCTTGTTATGTAGTGGTTTGTTTGCAGTGATGTATCTAACTAACTATCTGTTTAGTATAGACCAATCAACAATCCAGTTAATTACCTTTGTTTATCTGTTTTGGCTCTTTTTTGCAGCTAAGGTATCACAAACAACAACTTGGACTTACTTTTACTGGTTTAAAAGGAGCTTAAAGGACAAGGTATTTATCAAAAACTTATTTAACGATAACTTCAACTCGCAGTGGAAGATAGATCTGATTGAAAGTTCTAGCTTAGTTTTCATTTTTATTATCTACAGTGGTTTTAACTTTGGTGGGATTGATGGCAACTTTAACCTTGTGATCTTTTATCTAATTGCTATTGTTGGTTTATTTGATGTTGCAACTGCTTTTTTACCAATGTTTTGCTTTGGTTTAATCAATGGTTGA", "aa": "MNTDFFITKNQHDFFLKNNWKFLFVWLILLAIGVVMLVLYLVQPNLLGEFLAADVESSNGIIAGIGIVSLLYLAYCLIRYGVAYCLSYLVSFILLCSGLFAVMYLTNYLFSIDQSTIQLITFVYLFWLFFAAKVSQTTTWTYFYWFKRSLKDKVFIKNLFNDNFNSQWKIDLIESSSLVFIFIIYSGFNFGGIDGNFNLVIFYLIAIVGLFDVATAFLPMFCFGLING", "aa_hexdigest": "9a4891a76217b07c8c1a4a4160d0d815", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26266.718400000016, "isoelectric_point": 5.678522300720215 }, "id": "JPCNHPFPBM_629", "locus": "GCA000292505_00608" }, { "type": "cds", "sequence": "contig_1", "start": 336213, "stop": 336647, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTAGTGATGGTACATACACCCCACCAAACTTTGTAACAGCAGCTACTGTTAAAGAGCTTTTAACAAATCCATTTCCCACAGCAGCTACCATTGCAACTGCAAAAACAAGTCTGGTTAACGCTCCACTTGCAAATACCATCACAGATGTTTCTAATTTTGCTTCTAGTTTTATTGCGTTTGGTATCATCGTTCTTATTGCTGCTGTTCTATTAACACTACGATACAAGCTCTTAGGGTTATACAAAGCATTAGCTTTAGGGTTAAGTGTGGTGTCATCACTGGTTATCTTTAGTGCAGTTGGTGGAGTTGTTGATGTCTTTTCTTTTGTGGGCATCTTCTTTGTTATTGCCATCAACCTAATTAATCTCTTGAGTTTAGGAGAGTTATTCTTAAGAAATATTAGAAATAATGCTTCTATTATCGAATCGTGA", "aa": "MSSDGTYTPPNFVTAATVKELLTNPFPTAATIATAKTSLVNAPLANTITDVSNFASSFIAFGIIVLIAAVLLTLRYKLLGLYKALALGLSVVSSLVIFSAVGGVVDVFSFVGIFFVIAINLINLLSLGELFLRNIRNNASIIES", "aa_hexdigest": "7a361ed3cafedc7a842d3582fdc6ca41", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15142.639899999991, "isoelectric_point": 8.06391506195068 }, "id": "JPCNHPFPBM_630", "locus": "GCA000292505_00609" }, { "type": "cds", "sequence": "contig_1", "start": 337191, "stop": 337646, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTAATGGGGGATTACCTTCTGTTACTCTCACCTTTGAACAAAACCGTGAGCAAGCCAAAACCCGCCAAAGAAAAAGACAAGTTGATGCTCAGGTTAGAAGAAATGCAATTAGTTCAGTTGAACACAACTACCAACTTTCACTTGAAACAACTGATGGAGTGGTTTTATTTGATTCATTGGATAATAATTTTATTAATGCTTCCATTAGAGCAGTTGTTCCCCAAAACACTTCTGTAAACAGTGCTTTAACCTTTGAATACAAGTTAAATAAAAATGTAGTTACCAAAGAAAGTTTACACACCGATTTTCTTGATTTTATCAACTCTAAAAACTTATCAAGTTCTGATTATAATACTGGTAATGGGCAAGCTAGTGTTGAGGGCAATGGTAAATTTTTTAAGCAAAATGCGAATGGAACTTCAAGTAGTGGTAATAAAACTTTAGTATTGTGA", "aa": "MVNGGLPSVTLTFEQNREQAKTRQRKRQVDAQVRRNAISSVEHNYQLSLETTDGVVLFDSLDNNFINASIRAVVPQNTSVNSALTFEYKLNKNVVTKESLHTDFLDFINSKNLSSSDYNTGNGQASVEGNGKFFKQNANGTSSSGNKTLVL", "aa_hexdigest": "b97b2bf3ac8d845a7bbce7e6cb5459ff", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16649.161899999985, "isoelectric_point": 8.961830711364748 }, "id": "JPCNHPFPBM_631", "locus": "GCA000292505_00610" }, { "type": "cds", "sequence": "contig_1", "start": 337647, "stop": 337955, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGGTGTTATTTTTGGGAGTTACAAACTGTTAAATGACAGCAGGTTAGGTGCTGTTTTCAACGGTTCTACCACTACAACTGTTTACTTTTTAAACCATAAGTCAACTAACAATACCTCATTAGATCCTCAGCAAACAAAACCAACCAACGGGATTGAAAACATCACTAACATTGATAGCTTTTTAGATGGTGTTGAAAAAAGTTATGCTAACAGTTTATTCATTCAGGGCTTTTCTTCTGTAAACATTACTAAAAACACTAATGATAAAACTGCTAGTGAACTTGACAACATTGACAAGTCCTGA", "aa": "MFGVIFGSYKLLNDSRLGAVFNGSTTTTVYFLNHKSTNNTSLDPQQTKPTNGIENITNIDSFLDGVEKSYANSLFIQGFSSVNITKNTNDKTASELDNIDKS", "aa_hexdigest": "227633aa5314c59c3396f6de1da7e28e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11178.170399999988, "isoelectric_point": 5.107802009582519 }, "id": "JPCNHPFPBM_632", "locus": "GCA000292505_00611" }, { "type": "cds", "sequence": "contig_1", "start": 338073, "stop": 339461, "strand": "+", "frame": 3, "gene": "spoT", "product": "putative guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase", "db_xrefs": [ "COG:COG0317", "COG:TK", "EC:3.1.7.2", "GO:0008893", "GO:0015970", "SO:0001217", "UniRef:UniRef50_P75386", "UniRef:UniRef90_P47520" ], "nt": "ATGGCAACCATTCAGGAAATTGAGTGTGATTTTTTAGCTAAAATAGCACAAAAATTTACTAATGCAGAGATTGAATTAATTAACAAAGCATTCTATCACGCTAAAACTTGGCATGAAAACCAGAAACGGCTTAGCGGTGAACCTTTTTTTATCCATCCTTTAAGAACGGCATTATCACTAGTTGAATGGAACATGGATCCTATCACTATTTGTGCTGGTTTGTTACATGACATCATTGAAGATACAGACCAAACCGAAGCTAATATAGCAATGATTTTTAACAAAGAAATTGCTGAGCTTGTCACTAAGGTTACAAAGATTACCAATGAATCTAAAAAGCAACGTCATCTCAAAAATAAAAAGGAGAATCTTAACTTAAAAAGCTTTGTTAACATTGCAATCAATTCTCAACAAGAGATAAATGTAATGGTACTAAAACTAGCAGATCGACTTGATAACATCGCTTCCATTGAGTTTCTCCCCATTGAAAAGCAAAAGGTAATTGCAAAAGAAACTTTAGAACTTTATGCAAAGATTGCTGGGAGGATTGGGATGTATCCTGTTAAAACAAAATTAGCAGATCTTTCATTTAAGGTGTTGGATTTAAAAAACTATGATAACACCCTGTCAAAGATTAACAAGCAAAAGGTCTTTTATGACAATGAGTGGGATAACTTCAAACAACAATTAAAAAAAATCTTAGCGCAAAATCAGATAGAATACCAACTTGAAAGTCGGATTAAAGGCATTTACTCTACATATAAAAAACTAACTGTTCATGAACAGAACATCAGTAAGATCCATGATCTTTTTGCTATCCGCTTAATTACTAAATCAGAACTTGATTGTTATCACATCCTTGGTTTAATTCACCTTAATTTTTTAATTGACAGTAAATACTTCAAAGACTATATTGCCTCACCTAAACAAAACCTTTACCAATCAATTCATACCACTGTTCGTTTAAAAGGGTTAAATGTTGAGATCCAAATTAGAACCCAACAGATGGACAATGTTAGTAAGTTTGGCTTAGCTAGTCACTGGATCTACAAAGAACAGAAAGAGGGATTGTTAGCACCTGCTTTGCAACTTAATTACCTAGTGACAAAACAAAAACACTCACATGATTTTCTAAAAAGGATTTTTGGGACTGATATTATCAAGATTAATGTTAGTGCTAGTCATGAACCTAATGTAATTAAGCAAATTAATGTTGATAGCAACAATAAACTCCTTGATATTGCTTTTGAAAACTATCCCAAGCAATTTGCTAAATTAACCAAAATTGAAATTGATGGGGTTGAGATCAATTCTTTTGATACTAGTGTTGAAAATGAGATGCTGATTGAATTTTACTTTGGCAAGAATAACAATTTGAAATCAAAGTGA", "aa": "MATIQEIECDFLAKIAQKFTNAEIELINKAFYHAKTWHENQKRLSGEPFFIHPLRTALSLVEWNMDPITICAGLLHDIIEDTDQTEANIAMIFNKEIAELVTKVTKITNESKKQRHLKNKKENLNLKSFVNIAINSQQEINVMVLKLADRLDNIASIEFLPIEKQKVIAKETLELYAKIAGRIGMYPVKTKLADLSFKVLDLKNYDNTLSKINKQKVFYDNEWDNFKQQLKKILAQNQIEYQLESRIKGIYSTYKKLTVHEQNISKIHDLFAIRLITKSELDCYHILGLIHLNFLIDSKYFKDYIASPKQNLYQSIHTTVRLKGLNVEIQIRTQQMDNVSKFGLASHWIYKEQKEGLLAPALQLNYLVTKQKHSHDFLKRIFGTDIIKINVSASHEPNVIKQINVDSNNKLLDIAFENYPKQFAKLTKIEIDGVEINSFDTSVENEMLIEFYFGKNNNLKSK", "aa_hexdigest": "754fe49611551e69713014197dd74112", "start_type": "ATG", "rbs_motif": null, "genes": [ "spoT" ], "truncated": "3-prime", "pseudogene": { "start": 338073, "stop": 339764, "observations": { "mutation": [ 339459, 339726 ], "stop": [ 339459, 339726 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47520", "query_cov": 1.0, "subject_cov": 0.6416666666666667, "identity": 0.998, "score": 894.0, "evalue": 0.0, "gene_start": 1, "gene_end": 462, "reference_start": 1, "reference_end": 462, "reference_sequence": "MATIQEIECDFLAKIAQKFTNAEIELINKAFYHAKTWHENQKRLSGEPFFIHPLRTALSLVEWNMDPITICAGLLHDIIEDTDQTEANIAMIFSKEIAELVTKVTKITNESKKQRHLKNKKENLNLKSFVNIAINSQQEINVMVLKLADRLDNIASIEFLPIEKQKVIAKETLELYAKIAGRIGMYPVKTKLADLSFKVLDLKNYDNTLSKINKQKVFYDNEWDNFKQQLKKILAQNQIEYQLESRIKGIYSTYKKLTVHEQNISKIHDLFAIRLITKSELDCYHILGLIHLNFLIDSKYFKDYIASPKQNLYQSIHTTVRLKGLNVEIQIRTQQMDNVSKFGLASHWIYKEQKEGLLAPALQLNYLVTKQKHSHDFLKRIFGTDIIKINVSASHEPNVIKQINVDSNNKLLDIAFENYPKQFAKLTKIEIDGVEINSFDTSVENEMLIEFYFGKNNNLKSKWIRYMNNPIYREKVKKSLAKLAKSGRYSELAFYEKELGEKQLKLASETEIQKRLNTLRIKKMSDYLALIECTNFTNDEHLLFLAKNNDKWNKLTKPLKFAFSKVVFHNSYFEQIEGIFITKIVIEPCCSKIPDMPEQVTGILTKNILSVHRYGCKNLQNKKQLKIIPLYWNIQQLKLKPRKFRSYININGVWSEKTINKICQTIINGDGYIEKIIPKINKQKDEFDLNITLFVNNYQQLLTLMDQITTKNISFSWKYL" }, "paralog": false, "identity": 0.9928952042628775, "score": 1098.0, "evalue": 0.0, "description": "Internal stop codon at 339459, 339726. Nonsense mutation around 339459, 339726.", "psc": { "uniref90_id": "UniRef90_P47520", "gene": "spoT", "product": "Probable guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase", "ec_ids": [ "3.1.7.2" ], "uniref50_id": "UniRef50_P75386", "cog_id": "COG0317", "cog_category": "TK", "go_ids": [ "GO:0008893", "GO:0015970" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47520", "EC:3.1.7.2", "UniRef:UniRef50_P75386", "COG:COG0317", "COG:TK", "GO:0008893", "GO:0015970" ] }, "pscc": { "uniref50_id": "UniRef50_P75386", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75386" ], "product": "Probable guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase" } }, "id": "JPCNHPFPBM_633", "locus": "GCA000292505_00612" }, { "type": "cds", "sequence": "contig_1", "start": 339471, "stop": 339728, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAACCCTATATACCGTGAAAAGGTAAAAAAGAGCTTGGCTAAACTAGCTAAATCTGGTAGATACAGTGAGTTAGCTTCTTATGAAAAAGAACTGGGTGAAAAACAGTTAAAACTTGCTAGTGAAACTGAAATCCAAAAACGCTTAAACACCCTAAGAATTAAAAAAATGAGTGATTACTTAGCGTTAATTGAGTGTACTAACTTTACTAATGATGAACATTTGTTGTTTCTAGCTAAAAACAACGACAAGTGA", "aa": "MNNPIYREKVKKSLAKLAKSGRYSELASYEKELGEKQLKLASETEIQKRLNTLRIKKMSDYLALIECTNFTNDEHLLFLAKNNDK", "aa_hexdigest": "20724908d7aeebe3e00081759b8b6431", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9908.352199999987, "isoelectric_point": 9.380037117004395 }, "id": "JPCNHPFPBM_634", "locus": "GCA000292505_00613" }, { "type": "cds", "sequence": "contig_1", "start": 339825, "stop": 339968, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTGAACCATGTTGTAGTAAGATCCCTGATATGCCTGAACAAGTAACTGGTATCTTAACTAAAAACATTTTAAGTGTTCACCGTTATGGTTGTAAGAATTTACAAAATAAAAAGCAGTTAAAAATTATCCCGTTATATTGA", "aa": "MIEPCCSKIPDMPEQVTGILTKNILSVHRYGCKNLQNKKQLKIIPLY", "aa_hexdigest": "705a2ebcf5d94b3fa38f79fd7e531505", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5400.473400000001, "isoelectric_point": 9.21854381561279 }, "id": "JPCNHPFPBM_635", "locus": "GCA000292505_00614" }, { "type": "cds", "sequence": "contig_1", "start": 340029, "stop": 340223, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTGGAGTGAAAAAACCATTAATAAAATCTGTCAAACAATTATTAATGGTGATGGTTATATTGAAAAAATAATTCCCAAGATCAACAAACAAAAAGATGAATTTGATTTAAACATCACCCTTTTTGTTAATAACTACCAACAACTTCTCACCTTAATGGACCAAATTACCACTAAGAATATCAGCTTTAGTTGA", "aa": "MWSEKTINKICQTIINGDGYIEKIIPKINKQKDEFDLNITLFVNNYQQLLTLMDQITTKNISFS", "aa_hexdigest": "6eee32e0da4f977d666e149445b966b7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7510.615500000003, "isoelectric_point": 6.07798671722412 }, "id": "JPCNHPFPBM_636", "locus": "GCA000292505_00615" }, { "type": "cds", "sequence": "contig_1", "start": 340232, "stop": 340888, "strand": "-", "frame": 3, "gene": null, "product": "putative protein MG279", "db_xrefs": [ "GO:0005886", "RefSeq:WP_014894649.1", "SO:0001217", "UniParc:UPI00027B3ACC", "UniRef:UniRef100_UPI00027B3ACC", "UniRef:UniRef50_P47521", "UniRef:UniRef90_P47521" ], "nt": "ATGATTAGACTATTAAAAAAAACTTGCAGTTTTTTTATTATTTTGGTAGGAATTTTATTGTTAGGTGGAATTGCTACTGCTGGTTATTTTGCTTTTACTTACCGTGAACCTATTAACAACTACTATAAAGAGGGATATAACAAGATTAGTGAATATAACACTGAAATTAAAAAGATCTCCCAAAATATCTTCCAAAATAACCTAGTTAAAACACTTTCAGAAGTTGAAAAATCTTTAAACGAAGGGCGAAAATTAACGCAAAACAATTCATTTGCATCAGGGTTAGATAGTTCTTTAAATGCACTTGAAGGTTCATTGAAAAAAATTAATAACTTTGATAGTAATGCTGCTTTTACCCAAATTAAACACACATTAAATAACATAACTAGCTTTGTTGATCAAATGCTAGAAAAGTTCCCAAATCCAAACCAAAATGATGATTTTAAGCGGTATTTAACTGAAGTTTCCCAAATCCTTTTCTATACAGGAATCTCAATTATTGGGGCATTTTTTGTTTCTGGTTTTTTATTAATTTTATTTACAAAGAAAGTTTATGGAGTGAGAGTATCACGTTTCAATCCACAACGGCTACTAAAAAAGCACTTAGTTTTACTTTTAAGAGATGAAGAAGTGTATGATGCGGTGTTTGGTAACTAA", "aa": "MIRLLKKTCSFFIILVGILLLGGIATAGYFAFTYREPINNYYKEGYNKISEYNTEIKKISQNIFQNNLVKTLSEVEKSLNEGRKLTQNNSFASGLDSSLNALEGSLKKINNFDSNAAFTQIKHTLNNITSFVDQMLEKFPNPNQNDDFKRYLTEVSQILFYTGISIIGAFFVSGFLLILFTKKVYGVRVSRFNPQRLLKKHLVLLLRDEEVYDAVFGN", "aa_hexdigest": "4af76a00021851cff89382d92b50cc82", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3ACC", "ncbi_nrp_id": "WP_014894649.1", "uniref100_id": "UniRef100_UPI00027B3ACC", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3ACC", "RefSeq:WP_014894649.1", "UniRef:UniRef100_UPI00027B3ACC" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3ACC", "uniref90_id": "UniRef90_P47521" }, "psc": { "uniref90_id": "UniRef90_P47521", "product": "Uncharacterized protein MG279", "uniref50_id": "UniRef50_P47521", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47521", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47521" ], "product": "Uncharacterized protein MG279" }, "genes": [], "id": "JPCNHPFPBM_637", "locus": "GCA000292505_00616" }, { "type": "cds", "sequence": "contig_1", "start": 340881, "stop": 341678, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MG280", "db_xrefs": [ "GO:0005886", "RefSeq:WP_014894650.1", "SO:0001217", "UniParc:UPI00027B3ACD", "UniRef:UniRef100_UPI00027B3ACD", "UniRef:UniRef50_P47522", "UniRef:UniRef90_P47522" ], "nt": "ATGGTTATTTTTATTAATCGGTTTTCAAAAACCATCTTGCTTTTCTTTGGAATGTTGGTTTTTGTATTTCTGTTAGGGTTTGGTATTACTGCTTTGTATTTTCGTAGTACAGCAGCTAACCTTTATGTTCAAGCTCGTAATTCCATTGATAGTTCTTTCAATTCTGCTAAAGCCTTTGCTAATGCTTTAGCAAATAGTGCTAACCAATTTAGCAAAAGTTCCATTACTAACAATTTAGATCAAGTTAAAAAAGACTTAGAGCAATCTTTACAAAAAGTAGATGAATACAAAAAGAACTTAGAGAGTCAGAACAACCTAGGTAATATTAGTCAAGAAAAGATTAGAGAACTTGATGCAACCAAAAAAGACTTGGAAAACAGTAAAACACAATTAGAAAACTTTAAAAGTAATCTTGATAAAAATGGTACTGTTAGTTCATCTCCATCTGTAAAAAAAAAAGCAACCACTGATGGTGTAATTAGTGCAGTTAGTGAGTTTTCTACACAAGCACAAAGCATTGTTAGTAGCTATGAAAAGATCAAAAACAACATCCCTTCTTCTGAACAGTTCAATAATTATTATGATGTCACAATGATAACCATTGTTGCTGTATCAGGTGGGATGCTAGCAATTTTGATTACAACCATTGTCTTTTCCTTTTTAACTTCTAAAAAAAGGGGATTAATCAGGTTTTCAAGATTTACTAGTACTGAACAGTTAGCTGATCATGTTAATGATATCTTAGATCGTTATCCTGATTTGGAAGAGGAAGTGATAACAGCATTAGACCATGATTAG", "aa": "MVIFINRFSKTILLFFGMLVFVFLLGFGITALYFRSTAANLYVQARNSIDSSFNSAKAFANALANSANQFSKSSITNNLDQVKKDLEQSLQKVDEYKKNLESQNNLGNISQEKIRELDATKKDLENSKTQLENFKSNLDKNGTVSSSPSVKKKATTDGVISAVSEFSTQAQSIVSSYEKIKNNIPSSEQFNNYYDVTMITIVAVSGGMLAILITTIVFSFLTSKKRGLIRFSRFTSTEQLADHVNDILDRYPDLEEEVITALDHD", "aa_hexdigest": "076ae864c939f3881aaefc5746ebbfc7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3ACD", "ncbi_nrp_id": "WP_014894650.1", "uniref100_id": "UniRef100_UPI00027B3ACD", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3ACD", "RefSeq:WP_014894650.1", "UniRef:UniRef100_UPI00027B3ACD" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3ACD", "uniref90_id": "UniRef90_P47522" }, "psc": { "uniref90_id": "UniRef90_P47522", "product": "Uncharacterized protein MG280", "uniref50_id": "UniRef50_P47522", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47522", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47522" ], "product": "Uncharacterized protein MG280" }, "genes": [], "id": "JPCNHPFPBM_638", "locus": "GCA000292505_00617" }, { "type": "cds", "sequence": "contig_1", "start": 341739, "stop": 342569, "strand": "-", "frame": 2, "gene": null, "product": "IgG-blocking protein M", "db_xrefs": [ "GO:0005515", "GO:0016020", "SO:0001217", "UniRef:UniRef50_P47523", "UniRef:UniRef90_P47523" ], "nt": "TTGCAATTACCAGAATCAGTTAAAAAAGTTTCACTTTATGGTGATTACACTGGTGTTAATGTAGCTAAACAAATCTTTGCAAACGTAGTTGAGTTGGAATTTTATTCCACCAGTAAAGCAAATAGTTTTGGTTTTAACCCATTAGTATTGGGAAGTAAAACTAATGTTATCTATGATCTGTTTGCCTCAAAACCATTTACCCACATTGATTTAACCCAAGTTACACTCCAAAACAGTGATAATTCAGCCATTGATGCTAATAAGCTCAAACAAGCAGTTGGTGATATTTACAACTACAGAAGGTTTGAAAGACAGTTCCAAGGTTATTTTGCTGGGGGATACATTGACAAGTACTTAGTTAAGAATGTTAATACCAACAAGGATAGTGATGATGATTTGGTGTACCGTTCTTTAAAAGAACTTAACCTCCACTTAGAAGAAGCTTACAGAGAAGGAGATAACACCTATTACAGGGTTAATGAAAACTATTACCCTGGTGCTAGCATCTATGAAAATGAACGTGCAAGCAGAGACAGCGAGTTTCAAAATGAAATTTTAAAGCGTGCTGAACAAAACGGAGTAACATTTGATGAAAACATCAAAAGAATTACTGCTAGTGGTAAATACAGTGTACAGTTCCAAAAACTAGAAAATGATACAGATAGTAGTCTTGAAAGAATGACTAAAGCAGTTGAAGGGTTGGTTACTGTAATTGGTGAAGAAAAGTTTGAAACAGTTGATATTACTGGTGTTTCCAGTGATACAAATGAAGTTAAATCACTAGCAAAAGAACTTAAAACCAACGCATTGGGTGTTAAACTTAAACTCTAG", "aa": "MQLPESVKKVSLYGDYTGVNVAKQIFANVVELEFYSTSKANSFGFNPLVLGSKTNVIYDLFASKPFTHIDLTQVTLQNSDNSAIDANKLKQAVGDIYNYRRFERQFQGYFAGGYIDKYLVKNVNTNKDSDDDLVYRSLKELNLHLEEAYREGDNTYYRVNENYYPGASIYENERASRDSEFQNEILKRAEQNGVTFDENIKRITASGKYSVQFQKLENDTDSSLERMTKAVEGLVTVIGEEKFETVDITGVSSDTNEVKSLAKELKTNALGVKLKL", "aa_hexdigest": "2832ab3b78cfd7c2e74be53a2d48d8a9", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 341739, "stop": 342869, "observations": { "mutation": [ 342755 ], "stop": [ 342755 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47523", "query_cov": 1.0, "subject_cov": 0.49640287769784175, "identity": 0.996, "score": 535.0, "evalue": 1.6e-187, "gene_start": 1, "gene_end": 276, "reference_start": 281, "reference_end": 556, "reference_sequence": "MQFKKHKNSVKFKRKLFWTIGVLGAGALTTFSAVMITNLVNQSGYALVASGRSGNLGFKLFSTQSPSAEVKLKSLSLNDGSYQSEIDLSGGANFREKFRNFANELSEAITNSPKGLDRPVPKTEISGLIKTGDNFITPSFKAGYYDHVASDGSLLSYYQSTEYFNNRVLMPILQTTNGTLMANNRGYDDVFRQVPSFSGWSNTKATTVSTSNNLTYDKWTYFAAKGSPLYDSYPNHFFEDVKTLAIDAKDISALKTTIDSEKPTYLIIRGLSGNGSQLNELQLPESVKKVSLYGDYTGVNVAKQIFANVVELEFYSTSKANSFGFNPLVLGSKTNVIYDLFASKPFTHIDLTQVTLQNSDNSAIDANKLKQAVGDIYNYRRFERQFQGYFAGGYIDKYLVKNVNTNKDSDDDLVYRSLKELNLHLEEAYREGDNTYYRVNENYYPGASIYENERASRDSEFQNEILKRAEQNGVTFDENIKRITASGKYSVQFQKLENDTDSSLERMTKAVEGLVTVIGEEKFETVDITGVSSDTNEVKSLAKELKTNALGVKLKL" }, "paralog": false, "identity": 0.9973404255319149, "score": 741.0, "evalue": 1.87e-271, "description": "Internal stop codon at 342755. Nonsense mutation around 342755.", "psc": { "uniref90_id": "UniRef90_P47523", "product": "IgG-blocking protein M", "uniref50_id": "UniRef50_P47523", "go_ids": [ "GO:0005515", "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47523", "UniRef:UniRef50_P47523", "GO:0005515", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47523", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47523" ], "product": "Uncharacterized protein MG281" } }, "id": "JPCNHPFPBM_639", "locus": "GCA000292505_00618" }, { "type": "cds", "sequence": "contig_1", "start": 342753, "stop": 343409, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF20757.2" ], "nt": "ATGCAATTTAAAAAACATAAAAATAGTGTAAAATTTAAGCGTAAATTATTTTGGACTATTGGTGTTTTAGGTGCTGGAGCGTTAACCACATTCTCAGCAGTTATGATCACTAACCTAGTTAATCAAAGTGGTTATGCTTTGGTTGTACCAGGTAGGAGTGGTAACCTCGGTTTTAAGTTGTTCTCAACACAATCTCCTAGTGCAGAAGTAAAGTTGAAATCACTTTCTTTAAACGATGGTAGCTATCAGAGTGAGATTGATTTATCTGGTGGTGCTAACTTCCGTGAGAAATTTAGAAACTTTGCTAATGAGTTATCAGAAGCGATCACTAACAGTCCAAAGGGTTTAGACCGTCCTGTTCCTAAAACTGAAATTAGTGGCTTAATTAAAACAGGTGATAACTTTATTACTCCTAGTTTTAAAGCTGGTTATTATGATCATGTTGCTAGTGATGGTTCACTGCTTAGCTATTACCAATCAACTGAATATTTCAATAACCGCGTGTTGATGCCAATCTTGCAAACAACTAACGGTACTCTCATGGCAAACAACCGTGGTTATGATGATGTGTTTAGACAAGTTCCTAGTTTCAGCGGATGGAGTAACACAAAAGCAACAACTGTTTCAACTAGTAATAACCTTACTTATGATAAGTGA", "aa": "MQFKKHKNSVKFKRKLFWTIGVLGAGALTTFSAVMITNLVNQSGYALVVPGRSGNLGFKLFSTQSPSAEVKLKSLSLNDGSYQSEIDLSGGANFREKFRNFANELSEAITNSPKGLDRPVPKTEISGLIKTGDNFITPSFKAGYYDHVASDGSLLSYYQSTEYFNNRVLMPILQTTNGTLMANNRGYDDVFRQVPSFSGWSNTKATTVSTSNNLTYDK", "aa_hexdigest": "9ca2ffdcdb9139d3d66517dcac41af73", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF20757.2", "name": "M_large_dom", "length": 147, "aa_cov": 0.6422018348623854, "hmm_cov": 1.0, "evalue": 1.444787828667395e-85, "score": 282.54205322265625, "start": 79, "stop": 218 } ], "seq_stats": { "molecular_weight": 24019.7662, "isoelectric_point": 9.587431907653805 }, "id": "JPCNHPFPBM_640", "locus": "GCA000292505_00619" }, { "type": "tRNA", "sequence": "contig_1", "start": 343590, "stop": 343663, "strand": "-", "gene": "trnG", "product": "tRNA-Gly(tcc)", "amino_acid": "Gly", "anti_codon": "tcc", "score": 73.6, "nt": "GCGAGTATAGTTTAATGGTAGAACATCAGCCTTCCAAGCTGATCGTGTCGGTTCGATTCCGATTACTCGCTCCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 343629, 343631 ], "id": "JPCNHPFPBM_30", "locus": "GCA000292505_00620" }, { "type": "tRNA", "sequence": "contig_1", "start": 343671, "stop": 343756, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(taa)", "amino_acid": "Leu", "anti_codon": "taa", "score": 72.3, "nt": "GCCCAAGTGGCGGAATGGTAGACGCATGGGATTTAAGATCCCACGCCAGTAATGGTGTGCCGGTTCAAGTCCGGCTTTGGGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 343721, 343723 ], "id": "JPCNHPFPBM_29", "locus": "GCA000292505_00621" }, { "type": "tRNA", "sequence": "contig_1", "start": 343757, "stop": 343831, "strand": "-", "gene": "trnK", "product": "tRNA-Lys(ttt)", "amino_acid": "Lys", "anti_codon": "ttt", "score": 74.0, "nt": "GACTCACTAGCTCAGCGGTAGAGCATTTGACTTTTAATCAAAGGGTCCCGAGTTCGATCCTCGGGTGGGTCACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 343804, 343806 ], "id": "JPCNHPFPBM_28", "locus": "GCA000292505_00622" }, { "type": "tRNA", "sequence": "contig_1", "start": 343878, "stop": 343952, "strand": "-", "gene": "trnQ", "product": "tRNA-Gln(ttg)", "amino_acid": "Gln", "anti_codon": "ttg", "score": 70.6, "nt": "TGGGATGTAGCCAAGCGGTAAGGCAATAGACTTTGACTCTATCATGCGATGGTTCGATCCCATCCATCCCAGCCA", "db_xrefs": [ "SO:0000261" ], "anti_codon_pos": [ 343918, 343920 ], "id": "JPCNHPFPBM_27", "locus": "GCA000292505_00623" }, { "type": "tRNA", "sequence": "contig_1", "start": 343957, "stop": 344043, "strand": "-", "gene": "trnY", "product": "tRNA-Tyr(gta)", "amino_acid": "Tyr", "anti_codon": "gta", "score": 72.6, "nt": "GGACAGGTAGCGAAGTGGCTAAACGCTTCTGACTGTAGATCAGACACCTTTATGGTTTCGGGAGTTCGAATCTCTCCCTGTCCACCA", "db_xrefs": [ "SO:0000272" ], "anti_codon_pos": [ 344035, 344037 ], "id": "JPCNHPFPBM_26", "locus": "GCA000292505_00624" }, { "type": "cds", "sequence": "contig_1", "start": 344071, "stop": 344556, "strand": "-", "frame": 1, "gene": "greA", "product": "transcription elongation factor GreA", "db_xrefs": [ "COG:COG0782", "COG:K", "GO:0003677", "GO:0006354", "GO:0032784", "GO:0070063", "RefSeq:WP_009885993.1", "SO:0001217", "UniParc:UPI000012BAA4", "UniRef:UniRef100_P47524", "UniRef:UniRef50_P47524", "UniRef:UniRef90_P47524" ], "nt": "ATGGAACTAAATAAAAATTACCTAACTCAAGAAGGATTTAAGCAACTGGAAAAAGAACTTGAAAACCTAATCCAAGTTAAACGTCCTGAGATTATCAGACTCTTACAAGAAGCACGTGATCAGGGTGATTTAAGTGAAAATGCTGATTATGATGCAGCTAAAGCACAGCAAGGTGAGATTGAAACTAGGATTGCTGAAATTCAAGATATATTAGCCAACGCTAAGTTAATTAGTGACCACCAAGCAAAAACCAAAGTAACAAAAGTTAGCTTAGGGAGCACTGTTGAGATCTATGATTACAGTTCTAAATCCAACGAAAAATACACAATTGTAGGTACACTTGAAGCAAATCCTGAAGAACACAAAATTTCCAATGAATCACCCCTTGCCCTTGCAATCTATGGGCGTTTAATTGGTGATGAATGTGATGTTGTTGGTATTGAAGTTCCCTATCGTGTTAAAATCCTGAAGATCAGCAACAGATAA", "aa": "MELNKNYLTQEGFKQLEKELENLIQVKRPEIIRLLQEARDQGDLSENADYDAAKAQQGEIETRIAEIQDILANAKLISDHQAKTKVTKVSLGSTVEIYDYSSKSNEKYTIVGTLEANPEEHKISNESPLALAIYGRLIGDECDVVGIEVPYRVKILKISNR", "aa_hexdigest": "d2968165f7cac4163cfd0cda1e5c4990", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012BAA4", "ncbi_nrp_id": "WP_009885993.1", "uniref100_id": "UniRef100_P47524", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012BAA4", "RefSeq:WP_009885993.1", "UniRef:UniRef100_P47524" ] }, "ips": { "uniref100_id": "UniRef100_P47524", "uniref90_id": "UniRef90_P47524" }, "psc": { "uniref90_id": "UniRef90_P47524", "gene": "greA", "product": "transcription elongation factor GreA", "uniref50_id": "UniRef50_P47524", "cog_id": "COG0782", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0006354", "GO:0032784", "GO:0070063" ] }, "pscc": { "uniref50_id": "UniRef50_P47524", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47524" ], "product": "Transcription elongation factor GreA" }, "genes": [ "greA" ], "id": "JPCNHPFPBM_641", "locus": "GCA000292505_00625" }, { "type": "cds", "sequence": "contig_1", "start": 344737, "stop": 344871, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W775" ], "nt": "ATGAGAGAAAACAATAGTAATGCTCAAAACAAAAAGAAGATCCGCTTTTTAGCTTTGGGCATCATTATTCTAATCGTACTAATTGCTTTAATTACTATCCTTTTTATCACTGGAGTAATTAGTCGTAACCGTTAA", "aa": "MRENNSNAQNKKKIRFLALGIIILIVLIALITILFITGVISRNR", "aa_hexdigest": "4bf0e6366125a119729b772762aae7a0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AB38W775", "query_cov": 0.9090909090909091, "subject_cov": 0.8695652173913043, "identity": 0.675, "score": 49.7, "evalue": 3.98e-07, "valid": false, "uniref50_id": "UniRef50_A0AB38W775" }, "pscc": { "uniref50_id": "UniRef50_A0AB38W775", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AB38W775" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4993.057099999998, "isoelectric_point": 11.999967765808105 }, "id": "JPCNHPFPBM_642", "locus": "GCA000292505_00626" }, { "type": "cds", "sequence": "contig_1", "start": 345011, "stop": 345337, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAATCCAACAGCTTTACAATGATGCAATTGCAAATAAAAATCAATTGTCTGCAATTGCTCTAACTAAATTCCAACCAACTACTAGTTTTTGTTATCAAGTATTCCAAGTACAACTCCCTACCCTTTCTTTTTACAGTGAATATCAAAAGGAAAAAACCCATATCAAAGGTTTTAATCCTGAGCTTTTTTTAATTAATCAAGTTGGTCAAAAACAACTCAATGATCCTTTGGTTTTACGACCTACTAGTGAGATTGCTTTTTGCAACTTATGGAAAAAACAAGAGTTATCTTACCATGATCTACCTTTAATTTATAACCAGTGA", "aa": "MEIQQLYNDAIANKNQLSAIALTKFQPTTSFCYQVFQVQLPTLSFYSEYQKEKTHIKGFNPELFLINQVGQKQLNDPLVLRPTSEIAFCNLWKKQELSYHDLPLIYNQ", "aa_hexdigest": "82917a68bea6990deb1af9ee76d9273a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12659.35619999999, "isoelectric_point": 6.70810375213623 }, "id": "JPCNHPFPBM_643", "locus": "GCA000292505_00627" }, { "type": "cds", "sequence": "contig_1", "start": 345518, "stop": 346339, "strand": "+", "frame": 2, "gene": "proS", "product": "proline--tRNA ligase", "db_xrefs": [ "COG:COG0442", "COG:J", "EC:6.1.1.15", "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433", "GO:0017101", "KEGG:K01881", "SO:0001217", "UniRef:UniRef50_P47525", "UniRef:UniRef90_P47525" ], "nt": "TTGAAAAGTGAAAGTGAAAAATTTGCAGGTGCTAAAAACACATGGACAATAGAAGCAATTATGCCTGATGGACAAAGTTTGCAATGTGCCACTAGCCATGATTTAAGTGACACTTTTACAAAGAGTTTTACTATCAGCTATCAGAGTAAAACTAACCAAAAAATGACTCCAAGTAGTTTTAGTTGTGGGATGTCAACTAGGATCTTAGGAGCAATTTTTTTAACCCACAGCGATGATTATGGTTTGGTTTTACCTTGGTATCTAGCAAGTAAACAAGTCAAGTTATACCTGTTTGATAAAAACAATAACCCTAAAACAAGAGCTTTAGCTTTTTTAGTGAAGGATTTTTTAGAAAAACTCAAAATTCGCTTTAGTTTTATAGAAATTAACAATCAACTAGGTAAACAACTTTTAAAAGGAGAAATAGAAGGTATTCCACTACAGATGATTGTTGATAATGAAAAAACTATTAACATCTTCAACCGCTTAACACGTTTAAAAACCAGCTTAACATTTGCAAATCTTCAAACTGAATTTGTTAATTTAGTTGAAAACTACCATACAGAGATGTATAGAAAAGCAAATGATTTAGTTGAACAAAAACTAGCAAGAGTACAAACTTTAAAGGAAATTGAACAAGCATTCAAAAATAAAAAGGCTGTTTTATGTACCGTTAAGTTAACTGGTGAACTTGAACAACACTTAAAGACAAAATACCAAGTTAGTGTTAGGTGTTTTTTTAAAAAGTCAGATGTAACACAAAACTGTCCTTTTACAAATCAACCTTGTTTTGATTCAGTTTTAATTGCACGTGCTTACTAA", "aa": "MKSESEKFAGAKNTWTIEAIMPDGQSLQCATSHDLSDTFTKSFTISYQSKTNQKMTPSSFSCGMSTRILGAIFLTHSDDYGLVLPWYLASKQVKLYLFDKNNNPKTRALAFLVKDFLEKLKIRFSFIEINNQLGKQLLKGEIEGIPLQMIVDNEKTINIFNRLTRLKTSLTFANLQTEFVNLVENYHTEMYRKANDLVEQKLARVQTLKEIEQAFKNKKAVLCTVKLTGELEQHLKTKYQVSVRCFFKKSDVTQNCPFTNQPCFDSVLIARAY", "aa_hexdigest": "738ac7d3ecf02a540f4a590b2430f628", "start_type": "TTG", "rbs_motif": null, "genes": [ "proS" ], "truncated": "5-prime", "pseudogene": { "start": 345218, "stop": 346339, "observations": { "mutation": [ 345335, 345398, 345461 ], "stop": [ 345335, 345398, 345461 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47525", "query_cov": 1.0, "subject_cov": 0.5652173913043478, "identity": 0.985, "score": 530.0, "evalue": 1.09e-186, "gene_start": 1, "gene_end": 273, "reference_start": 211, "reference_end": 483, "reference_sequence": "MTKKTQDLTSWYDQLLVKAKLICHGEVKGTVCFLNNSWGLWMEIQQLYNDAIANKNQLSAIALTKFQPTTSFCYQVFQVQLPTLSFYSEYQKEKTHIKGFNPELFLINQVGQKQLNDPLVLRPTSEIAFCNLWKKQELSYHDLPLIYNQWTQVFRAEKNTRPFLRNSEFYWQETHGLFVDQSQSEQAAISFWNLYQDLIINKLCIPAFVGLKSESEKFAGAKNTWTIEAIMPDGQSLQCATSHDLGDTFTKSFTISYQSKTNQKMTPSSFSCGMSTRILGAIFLTHSDDYGLVLPWYLASKQVKLYLFDKNNNPKTRALAFLVKDFLEKLKIRFSFIEINNQLGKQLLKGEIEGIPLQMIVDNEKTINIFNRLTRLKTSLTFANLQTEFVNLVNNYHTEMYRKANDLVEQKLARVQTLKEIEQAFKNKKAVLCTVKLTGELEQHLKTKYQVSVRCVFKKSDVTQNCPFTNQPCFDSVLIARAY" }, "paralog": false, "identity": 0.9812332439678284, "score": 729.0, "evalue": 8.46e-268, "description": "Internal stop codon at 345335, 345398, 345461. Nonsense mutation around 345335, 345398, 345461.", "psc": { "uniref90_id": "UniRef90_P47525", "gene": "proS", "product": "proline--tRNA ligase", "ec_ids": [ "6.1.1.15" ], "uniref50_id": "UniRef50_P47525", "cog_id": "COG0442", "cog_category": "J", "kegg_orthology_id": "K01881", "go_ids": [ "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433", "GO:0017101" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47525", "EC:6.1.1.15", "UniRef:UniRef50_P47525", "COG:COG0442", "COG:J", "KEGG:K01881", "GO:0004827", "GO:0005524", "GO:0005737", "GO:0006433", "GO:0017101" ] }, "pscc": { "uniref50_id": "UniRef50_P47525", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47525" ], "product": "Proline--tRNA ligase" } }, "id": "JPCNHPFPBM_644", "locus": "GCA000292505_00628" }, { "type": "cds", "sequence": "contig_1", "start": 346746, "stop": 346886, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAAAAAGAAACTGTTGATTTTATCTATGACATCTATATTTATGGCAAAAAGCCGTGTGATATTGGTTATTCTAATTCCACCTATTACAAAAAACTAAACAAAGCAGCTAATAGTTTTTTTGACCATTTTGTTTGA", "aa": "MLKKETVDFIYDIYIYGKKPCDIGYSNSTYYKKLNKAANSFFDHFV", "aa_hexdigest": "f2deb3d58c90f77abbe22626051eb867", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5480.226300000001, "isoelectric_point": 8.734063911437985 }, "id": "JPCNHPFPBM_645", "locus": "GCA000292505_00629" }, { "type": "cds", "sequence": "contig_1", "start": 346921, "stop": 347154, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGCAATTCTCAACGTACAACAAATTCAAAATAACCAAGTTACTGAGTACACAATGAGTCCAGTGCGTAATTTTGCAAACACAAAAGATGTATATTTTGATGCGCAATTAACAAATATAGAAAGCAAAATTGATAGTAGTAGGGCTCAAATTCACCTTACTATTGCACTAAAATACAACACTAATCTCCCTGATAATATCTTTCAAGCCCACTTCAGTTTAGGCAATTGA", "aa": "MAAILNVQQIQNNQVTEYTMSPVRNFANTKDVYFDAQLTNIESKIDSSRAQIHLTIALKYNTNLPDNIFQAHFSLGN", "aa_hexdigest": "973c19d15852bbfc7b85d292edc6fd44", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8744.706699999995, "isoelectric_point": 5.992500877380371 }, "id": "JPCNHPFPBM_646", "locus": "GCA000292505_00630" }, { "type": "cds", "sequence": "contig_1", "start": 347365, "stop": 347502, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGATACTGTAGCTAGTGAATTGTATGCAACAGTAATTAAAGAAACAATCAACTTTGGCAACATGATTAAGATTAATGTACTTGATGGTAGTAAACAGCTAACTAGTAGCCAAGGAAGTTTTAAATACAGTTGA", "aa": "MFDTVASELYATVIKETINFGNMIKINVLDGSKQLTSSQGSFKYS", "aa_hexdigest": "9d2eb7ecbb8b2a362ba724978eebaee4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4977.618600000001, "isoelectric_point": 5.970674705505371 }, "id": "JPCNHPFPBM_647", "locus": "GCA000292505_00631" }, { "type": "cds", "sequence": "contig_1", "start": 347506, "stop": 347715, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGACTATAGGAATTTAGAACAACTTGATGAGGTGAGAAACTTGATCAATATCAGTTTTGACAAACCAGTTCAAATAGTAAATGTTGATGTCAAAATTCACTATGTTCCAACTAAAGGTAGGTTACAAGAGATAAAACAACAAGGTGAGTTTGAAAATAACCTTGATGTTAATGAAAAGCTTAAACTCAATTTAATAGGCAATTGA", "aa": "MYDYRNLEQLDEVRNLINISFDKPVQIVNVDVKIHYVPTKGRLQEIKQQGEFENNLDVNEKLKLNLIGN", "aa_hexdigest": "c574042e20a6344067741eb2cd5cc493", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8157.187999999998, "isoelectric_point": 5.236087608337402 }, "id": "JPCNHPFPBM_648", "locus": "GCA000292505_00632" }, { "type": "cds", "sequence": "contig_1", "start": 347806, "stop": 347964, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGGCGTTACAGTAGGTAATGATTTCTACACAATTATAGCCAACAACCAGTTTAAGTACGAAACACCTTTAGATGATCTTGAACAAAATGACTTTTTTGAGGTAAATTATTTACCTGTTTACAGTACATACAACTTTAGTGATTTAACCCAGTAA", "aa": "MIGVTVGNDFYTIIANNQFKYETPLDDLEQNDFFEVNYLPVYSTYNFSDLTQ", "aa_hexdigest": "ec53013ec041fff8439c5c6e9124735d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6108.613000000001, "isoelectric_point": 4.0500284194946286 }, "id": "JPCNHPFPBM_649", "locus": "GCA000292505_00633" }, { "type": "cds", "sequence": "contig_1", "start": 347967, "stop": 348479, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MG286", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_P47528", "UniRef:UniRef90_P47528" ], "nt": "ATGATTTTTTCAATTAGTAAACGAAAGTTAATTTGCGGATTTTTGCTAGTTATTCTAACAATAGGTGGGGTTTTGGGTGGTGTTTATTTAGTTACTAAAAACAACAAGGATAACTACCAAAATGAAAGTAATTTCAACAATCAAGAACAAATTAGTAAAATCCCTAATTTCAAAGCTATTGGTCCTGAAACACAACGTATCTTAAGAGAAAGAAACTATCCTTTAGATGATAGTGGTTATTATGTTTATAAGTATGGTGAAATTAATAGGTATCTAAGAAATGAAAGTGAACTAGATGAATTGATTAACTATAGAGTGATGGTACCTTCACTTAAATCACATCACAAAAGGGTTAATTTTGATAAGGCATTTCTTGAAAGTAAATTAAGAAAATGGATTATCAAAGCAATTAAGCAACATAACTATTTCCAACACTTTGAAAATGAACCAAATTTAAGAGTTCAATATAACATGAATATTCCAGCACAAAAAATTGATGTTAATGCTGTTTGA", "aa": "MIFSISKRKLICGFLLVILTIGGVLGGVYLVTKNNKDNYQNESNFNNQEQISKIPNFKAIGPETQRILRERNYPLDDSGYYVYKYGEINRYLRNESELDELINYRVMVPSLKSHHKRVNFDKAFLESKLRKWIIKAIKQHNYFQHFENEPNLRVQYNMNIPAQKIDVNAV", "aa_hexdigest": "f448b61c050120ae22a034f1def54deb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47528", "query_cov": 1.0, "subject_cov": 0.8673469387755102, "identity": 0.9940000000000001, "score": 338.0, "evalue": 2.23e-117, "valid": true, "product": "Uncharacterized protein MG286", "uniref50_id": "UniRef50_P47528", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47528", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47528" ], "product": "Uncharacterized protein MG286" }, "genes": [], "id": "JPCNHPFPBM_650", "locus": "GCA000292505_00634" }, { "type": "tRNA", "sequence": "contig_1", "start": 348833, "stop": 348907, "strand": "+", "gene": null, "product": "tRNA-Xxx", "score": 70.7, "nt": "AGGGGTATAGTTCAAAGGTAGAACATCTGTCTTCAAAATAGAGTGTTGTGGGTTCGAGTCCTGCTACCCCTGCCA", "db_xrefs": [], "id": "JPCNHPFPBM_12", "locus": "GCA000292505_00635" }, { "type": "cds", "sequence": "contig_1", "start": 348978, "stop": 349331, "strand": "+", "frame": 3, "gene": null, "product": "adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7M3VDH2", "UniRef:UniRef90_UPI001EE65182" ], "nt": "GTGTTACTAGTGCCTAAGGTGGTAGTGGAGTATCACCAACTTGATAAGAAGGTTGTCAAAGAGAGTTTGGAAGTGGAAGCAACTGATTCCTTCAACCCCACCCAAAGGTTGCAAAAAGATAGTCCAATGAAGGATTCAACAAAAACAGGGGAGAAACTCCAAGAGACCATGTCATCAATGAGTGGTGATGGGGCTACATCCACTCGCAAGGCCCTCAAAGTGGAGGTGGAAAGGGGGAGTAAAGTCAATCAAGGCGAACTTTTAAAAAACGACTTTGCTAAAAAGCCACTGAAACATAAAGAAAATAGTGGGGAAGTGAAGTTAGATGCGAGTGGGGAGTTTACTGAGGCCTGA", "aa": "MLLVPKVVVEYHQLDKKVVKESLEVEATDSFNPTQRLQKDSPMKDSTKTGEKLQETMSSMSGDGATSTRKALKVEVERGSKVNQGELLKNDFAKKPLKHKENSGEVKLDASGEFTEA", "aa_hexdigest": "53af021367fe615d9e090fb26a456f7b", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00030561CF", "query_cov": 0.9658119658119658, "subject_cov": 0.8625954198473282, "identity": 0.8140000000000001, "score": 163.0, "evalue": 6.36e-50, "valid": false, "product": "adhesin", "uniref50_id": "UniRef50_A0A7M3VDG5" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 348987, "stop": 349520, "observations": { "mutation": [ 349329, 349386, 349509 ], "stop": [ 349329, 349386, 349509 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001EE65182", "query_cov": 0.9658119658119658, "subject_cov": 0.6726190476190477, "identity": 0.858, "score": 169.0, "evalue": 1.51e-51, "gene_start": 5, "gene_end": 116, "reference_start": 2, "reference_end": 113, "reference_sequence": "MPKVVVEYHQLDKKVVKESLEVEATSTFNPTQRLKSDSPMKDSTKTGEKLQETMSSMSGMATSTRKALKVEVERGSNVNQGELAKNDFAKKPLKHKNSSGTDVKLDSQKDFTEGKVWKPVLTTDEIKKNRGMGAFANSPSPSTSASSLPHSPLFLTSMLGLNQWSLNT" }, "paralog": false, "identity": 0.7277777777777777, "score": 228.0, "evalue": 1.13e-77, "description": "Internal stop codon at 349329, 349386, 349509. Nonsense mutation around 349329, 349386, 349509.", "psc": { "uniref90_id": "UniRef90_UPI001EE65182", "product": "adhesin", "uniref50_id": "UniRef50_A0A7M3VDH2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001EE65182", "UniRef:UniRef50_A0A7M3VDH2" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7M3VDH2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7M3VDH2" ], "product": "MgpB adhesin protein" } }, "id": "JPCNHPFPBM_651", "locus": "GCA000292505_00636" }, { "type": "cds", "sequence": "contig_1", "start": 349345, "stop": 349515, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCATTGATTTAACCCCGACATTGATGTTAGAAAAAGTGGGGAGTGGGGTAGAAGAAGAAGCAGAAAGGGTAGCAGTGTGGGAGTTGGAGAAGGGTTTGTTGTTGCCGATTCAGGGGAGAAAGTCTAAGTCGCCCCCATCCCCCTATTATCTTTTATTTGTTCGGTAG", "aa": "MIIDLTPTLMLEKVGSGVEEEAERVAVWELEKGLLLPIQGRKSKSPPSPYYLLFVR", "aa_hexdigest": "d938eb9690fc95d3d836182b4d30e7ad", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6328.398499999999, "isoelectric_point": 5.259050559997559 }, "id": "JPCNHPFPBM_652", "locus": "GCA000292505_00637" }, { "type": "cds", "sequence": "contig_1", "start": 350308, "stop": 350484, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13", "UniRef:UniRef90_UPI002FCE54A6" ], "nt": "GTGGTGAAAAAATATCAGTTAGATGTACCGAATGTTACCAATGAGATGAACGATGTACTGAAAGAGTTGATCCTTGAACAACCTTTGACTAAGTATACCTTAAATTCGAGTTTGGCCAAAGAAAAGGGCAAAACGCAAGCAGTGGTGCATCTGGGTAGTGGTAAAGCAAATCAGTGA", "aa": "MVKKYQLDVPNVTNEMNDVLKELILEQPLTKYTLNSSLAKEKGKTQAVVHLGSGKANQ", "aa_hexdigest": "da5ea20f89e194c953143b0e2ca9ebf3", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 350164, "stop": 350549, "observations": { "deletion": [ 350500 ], "mutation": [ 350302, 350482 ], "stop": [ 350302, 350482 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FCE54A6", "query_cov": 1.0, "subject_cov": 0.46774193548387094, "identity": 0.81, "score": 91.7, "evalue": 1.31e-22, "gene_start": 1, "gene_end": 58, "reference_start": 47, "reference_end": 103, "reference_sequence": "MLFKSTQVKLTYSSSGSNNISFENNANKPSYVVEFTNSTTVGIKWSVVKKYQLDVPNVTNEMNQVLQELILEQPLTKYTLNGSLAKQKGKSQVAVHLGGQASQWHSTRHSQSKQQSQPQGFNRI" }, "paralog": false, "identity": 0.7421875, "score": 163.0, "evalue": 1.39e-53, "description": "Internal stop codon at 350302, 350482. Frameshift due to deletion around 350500. Nonsense mutation around 350302, 350482.", "psc": { "uniref90_id": "UniRef90_UPI002FCE54A6", "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FCE54A6", "UniRef:UniRef50_UPI00027B3D13" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3D13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3D13" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6456.401900000003, "isoelectric_point": 9.050474739074708 }, "id": "JPCNHPFPBM_653", "locus": "GCA000292505_00638" }, { "type": "cds", "sequence": "contig_1", "start": 350491, "stop": 350592, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTAATCAACACAACTTAGACAACAATCCCAGTCCCAATGCATCAACCGGATTTAAACTCACTACCGGCAATGCATATAGAAAATTAGATCAATCCTGA", "aa": "MRNQHNLDNNPSPNASTGFKLTTGNAYRKLDQS", "aa_hexdigest": "9f7100dd3d1242c098ec61834ea7e5b5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3690.967600000001, "isoelectric_point": 9.69399814605713 }, "id": "JPCNHPFPBM_654", "locus": "GCA000292505_00639" }, { "type": "cds", "sequence": "contig_1", "start": 350671, "stop": 350976, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAAAATGATGCGCCCAGTGTTACTGGAGGGGGATCAGATACTTCTTCAAAATTTAAAAGATACCTCAACACCAAACAAGCATTGGAAAGCATCGGCATCTTGTTTGATGGGGATGGAATGAGGAATGTTATCACCCAGCTCTATTATGCTTCTACCAGTAAGCTAGCAGTCACTAACAACCACATTGTCGTGATGGGTAACAGCTTTCTACCCAGCTTGTGGTACTGGGTGGTGGATCGGGGTGCAACAACTGATTCATCATCAAAACCCACCTGGTTTGCTAACACCAATTTAGATTGA", "aa": "MAKNDAPSVTGGGSDTSSKFKRYLNTKQALESIGILFDGDGMRNVITQLYYASTSKLAVTNNHIVVMGNSFLPSLWYWVVDRGATTDSSSKPTWFANTNLD", "aa_hexdigest": "67bad0ebf32b978dcaab739fc6ea5df8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11043.232299999992, "isoelectric_point": 8.00563564300537 }, "id": "JPCNHPFPBM_655", "locus": "GCA000292505_00640" }, { "type": "cds", "sequence": "contig_1", "start": 351254, "stop": 351523, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05220.18" ], "nt": "GTGACCAACGACACGGGTTTAAATGGTCCAATCAATGGGAGTTTTTCAATCCAAGACACCTTCAGCTTTGTTGTTCCTTATTCGGGGAATCATAACAATAATGGAACAACTGGACCCATTAAAACTGCTTATCCAGTGAAAAAAAGTGAAGCTTCAACAGTAATGATCAATTCCTTGATTAACGCTACGCCGTTGAATAGTTATGGGGATGAGGGGATTGGGGTTAGAAATATTTTTATGAGTTTAAAACAAGGTAATGCAGATAACTAG", "aa": "MTNDTGLNGPINGSFSIQDTFSFVVPYSGNHNNNGTTGPIKTAYPVKKSEASTVMINSLINATPLNSYGDEGIGVRNIFMSLKQGNADN", "aa_hexdigest": "ea893d0d573fbace1baeeb6ffccdd16a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05220.18", "name": "MgpC", "length": 75, "aa_cov": 0.8426966292134831, "hmm_cov": 1.0, "evalue": 1.5191755014608235e-22, "score": 75.13446807861328, "start": 3, "stop": 77 } ], "seq_stats": { "molecular_weight": 9427.318199999998, "isoelectric_point": 5.527614784240721 }, "id": "JPCNHPFPBM_656", "locus": "GCA000292505_00641" }, { "type": "cds", "sequence": "contig_1", "start": 351489, "stop": 351752, "strand": "-", "frame": 2, "gene": null, "product": "DUF237 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE37E3E", "UniRef:UniRef90_UPI002FE37E3E" ], "nt": "ATGGATGTTAAATTTATCGTTGATGGTGATTTGGATAGCTATTTAGTTCAAAATACTGATTATCCAGGTTCTTTATTTCAATTTAAAGACAATCAGCCACTTTTCACTTTACACGTAAAAGAGCAGATAAAAGTTCAAGATAAAAATTTTATGGATTTACTTAAACAGCAAAACTTACATAATTTAGATTTAAGAAATGGGGGTACAAAACCTCCAGTTGTTGATTTAGCTAGTTATCTGCATTACCTTGTTTTAAACTCATAA", "aa": "MDVKFIVDGDLDSYLVQNTDYPGSLFQFKDNQPLFTLHVKEQIKVQDKNFMDLLKQQNLHNLDLRNGGTKPPVVDLASYLHYLVLNS", "aa_hexdigest": "18fb2692669c5ce9a112a7083e67107a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 351489, "stop": 352004, "observations": { "mutation": [ 351764, 351785, 351806, 351902, 351995 ], "stop": [ 351764, 351785, 351806, 351902, 351995 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FE37E3E", "query_cov": 1.0, "subject_cov": 0.5087719298245614, "identity": 0.9890000000000001, "score": 174.0, "evalue": 2.53e-54, "gene_start": 1, "gene_end": 87, "reference_start": 85, "reference_end": 171, "reference_sequence": "MSDWLRDKLRSHTNISLNLNKVDVENKENNPTISWKEGGIDFRQEYPYKFKFEIDIKYQGGYELNFWSKFIGPWIGIPSSWKGEMDVKFIVDGDLDSYLVQNTDYPGSLFQFKDNQLLFTLHVKEQIKVQDKNFMDLLKQQNLHNLDLRNGGTKPPVVDLASYLHYLVLNS" }, "paralog": false, "identity": 0.9649122807017544, "score": 329.0, "evalue": 1.03e-117, "description": "Internal stop codon at 351764, 351785, 351806, 351902, 351995. Nonsense mutation around 351764, 351785, 351806, 351902, 351995.", "psc": { "uniref90_id": "UniRef90_UPI002FE37E3E", "product": "DUF237 domain-containing protein", "uniref50_id": "UniRef50_UPI002FE37E3E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FE37E3E", "UniRef:UniRef50_UPI002FE37E3E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FE37E3E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE37E3E" ], "product": "DUF237 domain-containing protein" } }, "id": "JPCNHPFPBM_657", "locus": "GCA000292505_00642" }, { "type": "cds", "sequence": "contig_1", "start": 352590, "stop": 353384, "strand": "-", "frame": 2, "gene": null, "product": "DUF237 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3AD5", "UniRef:UniRef50_UPI00027B3E39", "UniRef:UniRef90_UPI00027B3AD5", "UniRef:UniRef90_UPI00027B3E39" ], "nt": "TTGAAAGCTATCAAAAAATCTCGTTTTAAAATTAAAACTTTTCTCTTTGTCTCTTTTGCATCAACTTTAACTGCTGGTGTACTTATTGTCCCTTCTATCTATAATGGCTCAGCTGCAAATTTAAGTCAACCTAGTCTAAGAAAATCAAGTACAACAAATAGTAGAGAAAATAGTATTCTACTCCAACAGCAAACAGCTATAACAGAGCAAGAAGAAAACTATATTGCTAAAGAGAAAGATTATGATAAAAACTTTGACTTATTTCGAGAGAAATTTAAGAATGAAGTTTTAGATCCTAGGAACTTTAATGTTATAGATATTTTTCATCTTTTAAGTGGTTTTAAATCAGGTATTAAAAAAACAGTTGATTTATTAAATAGGCTGCAATCAAGCATTAATGAAACTAACAAAATTTTCCAATATGATGATGAAGATAAATTGCCTAAAGTTCCACAGAATTTTTTTAAGTTCCTTAATGGAAATTTCTTTCCACAATTGCATCCAAAAGGTTTAAACATTACTGAAAGTATTGTTGAAATATTTAATCAATACAACTTAAAGAAAATTCAATTAAAAGACTTTAATCTTGAATTGGTTAAAAAAAATGACATTGTTATTAAAGATAAGATTCGTTATAGCTTTGAAATCAAAATGGATTTTGAAGTTGATTATGAAGGTGGTAACAACGCTGTTAACTTGCAATTTGATTTAAAAGCCCAAACTTCTAATTTTTTGAATTTACAAGAGTTGCAAAATAGTTTTAATGGTAACGATATAAATACTCAATTGTTTTGA", "aa": "MKAIKKSRFKIKTFLFVSFASTLTAGVLIVPSIYNGSAANLSQPSLRKSSTTNSRENSILLQQQTAITEQEENYIAKEKDYDKNFDLFREKFKNEVLDPRNFNVIDIFHLLSGFKSGIKKTVDLLNRLQSSINETNKIFQYDDEDKLPKVPQNFFKFLNGNFFPQLHPKGLNITESIVEIFNQYNLKKIQLKDFNLELVKKNDIVIKDKIRYSFEIKMDFEVDYEGGNNAVNLQFDLKAQTSNFLNLQELQNSFNGNDINTQLF", "aa_hexdigest": "ed7b77f4e9a90ce46d2697662f5be9b6", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00027B3E39", "query_cov": 0.821969696969697, "subject_cov": 0.9194915254237288, "identity": 0.9259999999999999, "score": 379.0, "evalue": 3.94e-131, "valid": true, "uniref50_id": "UniRef50_UPI00027B3E39" }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3E39", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3E39" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 352353, "stop": 353384, "observations": { "mutation": [ 352592 ], "stop": [ 352592 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00027B3AD5", "query_cov": 1.0, "subject_cov": 0.4183835182250396, "identity": 1.0, "score": 506.0, "evalue": 2.82e-175, "gene_start": 1, "gene_end": 264, "reference_start": 1, "reference_end": 264, "reference_sequence": "MKAIKKSRFKIKTFLFVSFASTLTAGVLIVPSIYNGSAANLSQPSLRKSSTTNSRENSILLQQQTAITEQEENYIAKEKDYDKNFDLFREKFKNEVLDPRNFNVIDIFHLLSGFKSGIKKTVDLLNRLQSSINETNKIFQYDDEDKLPKVPQNFFKFLNGNFFPQLHPKGLNITESIVEIFNQYNLKKIQLKDFNLELVKKNDIVIKDKIRYSFEIKMDFEVDYEGGNNAVNLQFDLKAQTSNFLNLQELQNSFNGNDINTQLFWKPLINKLVDKNQNDLTSIAKTAVSDSLFLSNTNIFNSVLKIDEQLDLAKTKFEKEIIEPFKKEREKAKADYEEQQRILAEERRKQEEELKRKEEEAKRLKAEQEKFDKSLSTATEFKNYWSTKGKNVTDKTQLIDALKTSFAADKNKTFKFLIDSFTKSIKEYYKNNKKDENQNAKKAFGEKGIEFPKQGVEGLYMSDWLRDKLRSHTNISLNLNKVDVENKENNPTISWKEGGIDFRQEYPYKFKFEIDIKYQGGYELNFWSKFIGPWIGIPSSWKGEMDVKFIVDGDLDSYLVQNTDYPGSLFQFKDNQPLFTLHVKEQIKVQDKNFMDLLKQQNLHNLDLRNGGTKPPVVDLASYLHYLVLNS" }, "paralog": false, "identity": 0.9941690962099126, "score": 666.0, "evalue": 7.34e-241, "description": "Internal stop codon at 352592. Nonsense mutation around 352592.", "psc": { "uniref90_id": "UniRef90_UPI00027B3AD5", "product": "DUF237 domain-containing protein", "uniref50_id": "UniRef50_UPI00027B3AD5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00027B3AD5", "UniRef:UniRef50_UPI00027B3AD5" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00027B3AD5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00027B3AD5" ], "product": "DUF237 domain-containing protein" } }, "id": "JPCNHPFPBM_658", "locus": "GCA000292505_00643" }, { "type": "cds", "sequence": "contig_1", "start": 353726, "stop": 354178, "strand": "+", "frame": 2, "gene": "cypl", "product": "ABC transporter thiamine pyrophosphate-binding lipoprotein p37/Cypl", "db_xrefs": [ "GO:0005886", "GO:0009897", "GO:0030976", "SO:0001217", "UniRef:UniRef50_P75371", "UniRef:UniRef90_Q49410" ], "nt": "GTGCTTTTCAAAAAATTTACTTGGGTCATTCCAAGTTTGTTTTTAACAATTATTTCAACATCTTTACTTATTAGTTGTGCAACTAAAAGCGATAACACCTTAATATTCAATATTTCACTTGATCATAACGCTGATACATCAATAGAAAAATTCTTTACTGTTTTTTCAAAAAAACTTAGTGAAAAACTGAATAAAAAAATTAATGTTAACTTTAATATAGTTGATGATTCCTTTACAAAAATTAACAATATTCAAGCTAATAAGGCAGATTTTGCTTTTGTTAATTCGCAAGCTATTGCTTCAAATAATTGGTTTGGCTATACGCCATTAATACAAACTTTAACAACAGCTTTTAAAGAAGATTTGGAGCTTGATTATTATGAAGATGGTAATTTACAAAAAAAAGCTGAAAAAACGAATTTACTTTTTCTAAGTCCACCTTACAAAGAATGA", "aa": "MLFKKFTWVIPSLFLTIISTSLLISCATKSDNTLIFNISLDHNADTSIEKFFTVFSKKLSEKLNKKINVNFNIVDDSFTKINNIQANKADFAFVNSQAIASNNWFGYTPLIQTLTTAFKEDLELDYYEDGNLQKKAEKTNLLFLSPPYKE", "aa_hexdigest": "7be5d6fde783d297015932a993fbe979", "start_type": "GTG", "rbs_motif": null, "genes": [ "cypl" ], "truncated": "3-prime", "pseudogene": { "start": 353726, "stop": 354481, "observations": { "mutation": [ 354176, 354197, 354311, 354326 ], "stop": [ 354176, 354197, 354311, 354326 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49410", "query_cov": 1.0, "subject_cov": 0.4076086956521739, "identity": 0.94, "score": 271.0, "evalue": 1.76e-88, "gene_start": 1, "gene_end": 150, "reference_start": 1, "reference_end": 150, "reference_sequence": "MLFKKFTWVIPSLFLTIISTSLLISCATKSDNTLIFNISLDHNADTSIEKFFTVFSKKLSGKLNKKINVNFNIVDDSFTKINNIQANKADFAFVNSQAIASNNWFGYTPLIQTLTTAFKEDLELDYYEDGNLQKKAEKTNLLFLSPPYKEWDDIKQKWTGNRYDFLYEPSKLVSFYRSMILITGSASEITAIKKAWNEKNWNQFMKFGIGHGQTNSASRFELPDLLFRKHFAKNYPGLQNAINSDPDKFAVVRGREIGINKNIKIVFDDANSFSWTQNIKGSKRPFYTPIDPNDRLEILTYSDPLLYDIGIVSNNLSRIYQKAIGEIFIELAQSSEDLYGPSIGYNGYKMINDFEKEVVEIIEKTYGK" }, "paralog": false, "identity": 0.9442231075697212, "score": 466.0, "evalue": 8.66e-168, "description": "Internal stop codon at 354176, 354197, 354311, 354326. Nonsense mutation around 354176, 354197, 354311, 354326.", "psc": { "uniref90_id": "UniRef90_Q49410", "gene": "cypl", "product": "ABC transporter thiamine pyrophosphate-binding lipoprotein p37/Cypl", "uniref50_id": "UniRef50_P75371", "go_ids": [ "GO:0005886", "GO:0009897", "GO:0030976" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49410", "UniRef:UniRef50_P75371", "GO:0005886", "GO:0009897", "GO:0030976" ] }, "pscc": { "uniref50_id": "UniRef50_P75371", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75371" ], "product": "High affinity transport system protein p37" } }, "id": "JPCNHPFPBM_659", "locus": "GCA000292505_00644" }, { "type": "cds", "sequence": "contig_1", "start": 354338, "stop": 354550, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTTGGAATTGGTCATGGACAAACAAATTCAGCTTCACGTTTTGAGCTACCTGATCTTTTATTTAGAAAACATTTTGCTAAAAATTATCCCGGATTACAAAATGCAATTAATTCTGATCCCGATAAATTTGCCGTAGTTAGAGGAAGAGAGATAGGTATAAATAAAAACATCAAGATTGTTTTTGATGATGCTAATTCATTTTCTTGA", "aa": "MKFGIGHGQTNSASRFELPDLLFRKHFAKNYPGLQNAINSDPDKFAVVRGREIGINKNIKIVFDDANSFS", "aa_hexdigest": "cfa480c30db29582fa09225b1ac9e7b0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7853.800199999999, "isoelectric_point": 9.598198127746581 }, "id": "JPCNHPFPBM_660", "locus": "GCA000292505_00645" }, { "type": "cds", "sequence": "contig_1", "start": 354641, "stop": 354832, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATGACATTGGTATTGTTAGCAACAATTTATCAAGGATATATCAAAAAGCTATTGGTGAAATTTTTATTGAGTTAGCGCAATCAAATGAAGATCTATATGGGCCTTCAATTGGTTATAACGGCTATAAAATGATTAATGATTTTCAGAAAGAAGTTGTTGAAATAATTGAAAAAACCTATGGAAAATAA", "aa": "MYDIGIVSNNLSRIYQKAIGEIFIELAQSNEDLYGPSIGYNGYKMINDFQKEVVEIIEKTYGK", "aa_hexdigest": "12dc3e532ce2180790f2dbe48dd831ff", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7225.121799999999, "isoelectric_point": 4.648372459411622 }, "id": "JPCNHPFPBM_661", "locus": "GCA000292505_00646" }, { "type": "cds", "sequence": "contig_1", "start": 354822, "stop": 355154, "strand": "+", "frame": 3, "gene": "p29", "product": "putative ABC transporter ATP-binding protein p29", "db_xrefs": [ "COG:COG1120", "COG:PH", "GO:0005524", "GO:0016887", "GO:0042626", "GO:0043190", "SO:0001217", "UniRef:UniRef50_P47532", "UniRef:UniRef90_P47532" ], "nt": "ATGGAAAATAAACCAATTCTTTCTTTTGAAAAAGTATCAATAATATACAAAAAAGCTCCACTTTTGCAAAACATTAGTTTTAAAGTAATGGCAAAGGAAAATGTTTGCTTATTAGGTAAGTCTGGAGTTGGAAAATCAAGCCTTTTAAACAGTGTTACTAATACGAAAATAGTTAAAAGTGGGTTAGTTTACTTTGATGGTGTTGCTTCAAACAAAAAGGAATACAAAAAACTGAAAAAACAGTGCAGTTATCTGGATCAAATACCAAATTTAATTGACACTGATTATGTATATGAAGCAATTTTAAGATCTGCTAAACAAAAATTAACTTGA", "aa": "MENKPILSFEKVSIIYKKAPLLQNISFKVMAKENVCLLGKSGVGKSSLLNSVTNTKIVKSGLVYFDGVASNKKEYKKLKKQCSYLDQIPNLIDTDYVYEAILRSAKQKLT", "aa_hexdigest": "1d7b689a5bce4d2c65bbf50590f5ae49", "start_type": "ATG", "rbs_motif": null, "genes": [ "p29" ], "truncated": "3-prime", "pseudogene": { "start": 354822, "stop": 355457, "observations": { "mutation": [ 355152, 355185 ], "stop": [ 355152, 355185 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47532", "query_cov": 1.0, "subject_cov": 0.4489795918367347, "identity": 1.0, "score": 214.0, "evalue": 1.04e-68, "gene_start": 1, "gene_end": 110, "reference_start": 1, "reference_end": 110, "reference_sequence": "MENKPILSFEKVSIIYKKAPLLQNISFKVMAKENVCLLGKSGVGKSSLLNSVTNTKIVKSGLVYFDGVASNKKEYKKLKKQCSYLDQIPNLIDTDYVYEAILRSAKQKLTWLQKLICFEPKWIKDKILAILKEVNLNDYVSCIIKDLSAGQKQRVEIAKLFFKSPKLLLVDEPTTGLDPLTASKIMDLITDFVKREKITLVFVTHDIDLALKYSTRIIALKNHALVLDRLTEKLTKEQLYKIYDN" }, "paralog": false, "identity": 0.985781990521327, "score": 397.0, "evalue": 2.78e-143, "description": "Internal stop codon at 355152, 355185. Nonsense mutation around 355152, 355185.", "psc": { "uniref90_id": "UniRef90_P47532", "gene": "p29", "product": "Probable ABC transporter ATP-binding protein p29", "uniref50_id": "UniRef50_P47532", "cog_id": "COG1120", "cog_category": "PH", "go_ids": [ "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47532", "UniRef:UniRef50_P47532", "COG:COG1120", "COG:PH", "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_P47532", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47532" ], "product": "Probable ABC transporter ATP-binding protein p29" } }, "id": "JPCNHPFPBM_662", "locus": "GCA000292505_00647" }, { "type": "cds", "sequence": "contig_1", "start": 355377, "stop": 355559, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTAATTACTGATTTTGTGAAAAGAGAAAAAATAACTTTGGTTTTTGTAACACATGATATAGATTTAGCACTGAAATATAGCACCAGAATTATAGCACTTAAAAATCATGCTTTAGTGTTAGATAAATTAACAGAAAAACTAACAAAAGAACAACTTTATAAAATTTATGATAACTAA", "aa": "MDLITDFVKREKITLVFVTHDIDLALKYSTRIIALKNHALVLDKLTEKLTKEQLYKIYDN", "aa_hexdigest": "807d33cdfa63f82eaa9d723bf5f3a68f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7093.287699999999, "isoelectric_point": 8.004668617248537 }, "id": "JPCNHPFPBM_663", "locus": "GCA000292505_00648" }, { "type": "cds", "sequence": "contig_1", "start": 355866, "stop": 356105, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTCAAAAAGCTTTAAAACCACTTCCTTCTCTAATTTTTTGGTTAAAAAAATACTTTAAATTGCTGTTTTCAAAAAATGTAGTAATGTATTTTGTATTCCATAAAAAGCTAAACCAGCTAATCGTTAGTGTAGCTGCTAGTTGTTTATTAAATAAATTACTAAATAAAAAACCAAATAAAAGTACGGGAAATGCTCTTAAAACTATAGTTATTAACCTGATCGGTAGAGCAATGTAA", "aa": "MVQKALKPLPSLIFWLKKYFKLLFSKNVVMYFVFHKKLNQLIVSVAASCLLNKLLNKKPNKSTGNALKTIVINLIGRAM", "aa_hexdigest": "dc56122d310ee3d8bbad3e7935a9c231", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9020.056399999994, "isoelectric_point": 10.59965000152588 }, "id": "JPCNHPFPBM_664", "locus": "GCA000292505_00649" }, { "type": "cds", "sequence": "contig_1", "start": 356245, "stop": 356403, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAGTTTGATCTTGTATTAATTCCATTAGTTGTTTTAATAACCTTTCTAATTTTTATAGAAGTTGTTGTTTTTTTATTATCAAGTTTTATTTTTGAGAAAAATAGTGAAGATTTAAGACCAATTTTAAAAACAACAGTTATTGAAAAACGAAAGTGA", "aa": "MQFDLVLIPLVVLITFLIFIEVVVFLLSSFIFEKNSEDLRPILKTTVIEKRK", "aa_hexdigest": "e28d10b62760ea01af6a11f6add2d420", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6109.389899999998, "isoelectric_point": 6.09100284576416 }, "id": "JPCNHPFPBM_665", "locus": "GCA000292505_00650" }, { "type": "cds", "sequence": "contig_1", "start": 356422, "stop": 356916, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTATTGTAGTACTAATTAGTCTTAGTTTAGCCAATTTAGTGACTATTGATTACAGAATTAACGATGCTGAATTTTTACAAGATTTTTTTAACCACTTTTTTCAGTTAAAAAGTAATCTTTTTTCAAGTAATGATCCTAATATAAACCCAATTTTAATGTTAGTTAAACTTACAACTCAAGCTATTAGTTTAATTAGCTTGGTTGTAATCTTTTCTATTCTCTTTGGTTTTATATCATGTAATTTATTTAAAAAGAGATTTTCAATTAGTTTTAAGATCTTATTACTTTTTGTTAGAGTAGTTCCTAGTATTCTGTTGTTTAGGTTACTAGATCCTTTATTTCTAGAAGCAAAAACAACTATTATTTTAGTCTTATTAATTAATCATGGTTCAAGTTATGGCCAATTGATGGCAATTAACTTTAATAAGGCAAATCAAAATATCATCAATAACTATAAAAATCATGGTATGACAAAAGATTTTATTTTATGA", "aa": "MFIVVLISLSLANLVTIDYRINDAEFLQDFFNHFFQLKSNLFSSNDPNINPILMLVKLTTQAISLISLVVIFSILFGFISCNLFKKRFSISFKILLLFVRVVPSILLFRLLDPLFLEAKTTIILVLLINHGSSYGQLMAINFNKANQNIINNYKNHGMTKDFIL", "aa_hexdigest": "6172b086d5ffbd5f6d6750761689014a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18794.212999999992, "isoelectric_point": 9.561451148986816 }, "id": "JPCNHPFPBM_666", "locus": "GCA000292505_00651" }, { "type": "cds", "sequence": "contig_1", "start": 356923, "stop": 357180, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGTTGAAAATAAACCTAATTTAATAAACATTACCAGTGATGCTTATGATAGTGGAATTAGGGATTTAATTTTGTTTGGTAGTTTTGGCGGTTCAATTATTGGTAGTAGAATTACTAATTTTTTTGAAAGAGCTCAATTTGATAATCTAGGTTCTGTTACAATCCCATTAATGGTTTATCTAATTGCAATAGAAATAATTTTTCTTTCAGTTAGATTAACTAGAATTTCAGTTTTTAAGAACTACCTTTACTAA", "aa": "MLVENKPNLINITSDAYDSGIRDLILFGSFGGSIIGSRITNFFERAQFDNLGSVTIPLMVYLIAIEIIFLSVRLTRISVFKNYLY", "aa_hexdigest": "14c24271b3360f385bb04a95b41160dd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9610.111899999993, "isoelectric_point": 6.081283378601074 }, "id": "JPCNHPFPBM_667", "locus": "GCA000292505_00652" }, { "type": "cds", "sequence": "contig_1", "start": 357177, "stop": 357593, "strand": "-", "frame": 2, "gene": "ruvX", "product": "Holliday junction resolvase RuvX", "db_xrefs": [ "COG:COG0816", "COG:JLK", "EC:3.1.-.-", "EC:3.1.21.10", "GO:0000967", "GO:0004518", "GO:0005737", "GO:0006364", "RefSeq:WP_014894266.1", "SO:0001217", "UniParc:UPI00027B3AD9", "UniRef:UniRef100_UPI00027B3AD9", "UniRef:UniRef50_Q9ZB76", "UniRef:UniRef90_Q9ZB76" ], "nt": "GTGAAATATATATTAGCAATTGATTTTGGCTTGAAAAAAATAGGTACAGCAATTGCAAATACACTAGATAAATATCCTTCTGCATTTCATGTTTTTGAAGTAAAAAATAATTTCAAAACTGCTGTTAACAACTTATTTTTGCGAATTAAAAACGATGGGTATGAACTTGAAAAAATAGTGATAGGTTTTCCCAAATTTCACTATTATTCTGATATTCAAAAGGCAATTAAATCATTTAAACAACTTTTAGAACAACGTTTTAATTTGCCAATAATTTTGGTTGATGAAAGTAATACTACAAGTGCTGTTAAAGATAAGCTTATAACAATGGATTTAAAACATAAGGATTTTAAAAAGGCTAAAGATACATTAGCTGCAGTTTTAATATTAGAGCGCTTCTTTCAAAACTATCATTAG", "aa": "MKYILAIDFGLKKIGTAIANTLDKYPSAFHVFEVKNNFKTAVNNLFLRIKNDGYELEKIVIGFPKFHYYSDIQKAIKSFKQLLEQRFNLPIILVDESNTTSAVKDKLITMDLKHKDFKKAKDTLAAVLILERFFQNYH", "aa_hexdigest": "b6f0b927a65d008eb135df63a9afff66", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3AD9", "ncbi_nrp_id": "WP_014894266.1", "uniref100_id": "UniRef100_UPI00027B3AD9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3AD9", "RefSeq:WP_014894266.1", "UniRef:UniRef100_UPI00027B3AD9" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3AD9", "uniref90_id": "UniRef90_Q9ZB76" }, "psc": { "uniref90_id": "UniRef90_Q9ZB76", "gene": "ruvX", "product": "Holliday junction resolvase RuvX", "ec_ids": [ "3.1.-.-", "3.1.21.10" ], "uniref50_id": "UniRef50_Q9ZB76", "cog_id": "COG0816", "cog_category": "JLK", "go_ids": [ "GO:0000967", "GO:0004518", "GO:0005737", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZB76", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZB76" ], "product": "Putative pre-16S rRNA nuclease" }, "genes": [ "ruvX" ], "id": "JPCNHPFPBM_668", "locus": "GCA000292505_00653" }, { "type": "cds", "sequence": "contig_1", "start": 357597, "stop": 358097, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGATAGTTCAATTTTTAAAGTTGAGTTAAAAGAATTGCAACAAAGGCTAGATAAATTTATCTTACCTGAAAAAATTACCCAATTAAGAGATGCATCTGATACTTTATTAGCTTTAAAAAATGATATTAACCAGTTAAAAACAAAAAACTATAAAGTATCACAGCAAGCTTTAGCCTTATCAATTAAAAAGCAATTATTATCCTTAGTAGATAAAAATAAAAGTTATGTAATTGCTACTTTTAATGACGTAGAACCTAAACTATTGCTACAAACAATACATGATGTTTTCAATCAAAATCAAACTAAAAATTTCTTGATAATTAATCAATTCAATGGAAGTAATTCATTTATTGTTATAGGAAATAAAATTACCACCATTATTGAAAAATTAAGAAATAGTTTTAATTTAAAGGGCGGAGGCAATGATAAGTTATTTAGAGGTTCTTTTCAGGATAATGTTACCCCTCAAAAGCTTAATGAATTGTTTCAAAAATAA", "aa": "MIDSSIFKVELKELQQRLDKFILPEKITQLRDASDTLLALKNDINQLKTKNYKVSQQALALSIKKQLLSLVDKNKSYVIATFNDVEPKLLLQTIHDVFNQNQTKNFLIINQFNGSNSFIVIGNKITTIIEKLRNSFNLKGGGNDKLFRGSFQDNVTPQKLNELFQK", "aa_hexdigest": "4e9327ac6385fd105e403c193f2eac0d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19028.773699999998, "isoelectric_point": 9.667566108703614 }, "id": "JPCNHPFPBM_669", "locus": "GCA000292505_00654" }, { "type": "cds", "sequence": "contig_1", "start": 358200, "stop": 358487, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07973.19" ], "nt": "TTGAATCGTCATTTAACAAGAAATGAACTTGAGAAAGTAGAAAATAAAATTCGCTCTTTGATTAAACAAAAAATAAGCTCAGAAGAGATTTTTACTGATTTTGAAGGGAGTCAAAAACTAAATGCAATTGCTTATTTTGAAGAGGAATATTCTCAACATGAAATATTAAGAGTGATCCGCTTTGGTGATTATAGTGTTGAGTTGTGTGGTGGCACTCATGTAGCTAACACAGCTTCAATTGAAGATTGTTTTATTACTGATTTCTATTCTTTAGGAGCTGGAAGATGA", "aa": "MNRHLTRNELEKVENKIRSLIKQKISSEEIFTDFEGSQKLNAIAYFEEEYSQHEILRVIRFGDYSVELCGGTHVANTASIEDCFITDFYSLGAGR", "aa_hexdigest": "c3fd4e49eeefcf96237ada3ef9f3cf15", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07973.19", "name": "tRNA_SAD", "length": 31, "aa_cov": 0.3263157894736842, "hmm_cov": 1.0, "evalue": 1.3539994021677194e-12, "score": 42.600894927978516, "start": 56, "stop": 86 } ], "seq_stats": { "molecular_weight": 10952.125299999989, "isoelectric_point": 5.13400478363037 }, "id": "JPCNHPFPBM_670", "locus": "GCA000292505_00655" }, { "type": "cds", "sequence": "contig_1", "start": 358617, "stop": 359015, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCAAACATCGTTCTATCTCTAAGCAAAATAACCAAACTATAAATTTTGAAAAACAAAATATTAATTTAGTTAATTTCAAAACTAAAAGTACTTTTTTTTATCACAAAAATAAAATTAATGCTAAGGTAATTGGTCTTTTTGATGAAAATTATTTACCAGTTAAAGAACTTAATAATCAAAGTGGTTATGTAGTTTTTGACCAAACAGTTTTATATGCTACTTCTGGAGGACAGAGATATGATGAAGGAAGTTGCATTAATCATTCTAATAATAATGATCAAAAAATCAGTTTTCAAGGTGTATTTAAAGGACCTAATAAACAACACTTCCACTACTTTTTAGTAGGTAGTTTTAAACTCAATGATCAAGTAACTTTATCACATGATGAAACTTGA", "aa": "MAKHRSISKQNNQTINFEKQNINLVNFKTKSTFFYHKNKINAKVIGLFDENYLPVKELNNQSGYVVFDQTVLYATSGGQRYDEGSCINHSNNNDQKISFQGVFKGPNKQHFHYFLVGSFKLNDQVTLSHDET", "aa_hexdigest": "ef1292213d18431e6bc67b5fc9f8f670", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15240.800699999993, "isoelectric_point": 9.163229942321777 }, "id": "JPCNHPFPBM_671", "locus": "GCA000292505_00656" }, { "type": "cds", "sequence": "contig_1", "start": 359034, "stop": 359468, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAGAACAGAGTTATTTTCGGATTATTGCTGATCACTTTAAAGCAATCACTTTTACCATTTCAGAAGGAGTTTTACCTGGTCCTAATGAGAGAAATTATGTAGTAAGAAGACTTTTAAGACGTGCTTTAATAGCTTGTAAGAAATTGCAATTAAACTTAGCATTTATTGAAAAGATAATAGATGAAATCATCGCTTCATATGAGAATTATTATCAACATTTAAAAGCTAAAAATGAAACTGTTAAACAGGTAGTTTTAAAAGAGATTAATGCATTTAATAAAACGATTGATTTAGGTTTAGTGCTGTTTGAAAAAAGTGTTAAAAACAATACTCTAACTCCCCAATTAGCATTTCAATTGAACGAAACATACGGTTTTCCTGTTGAAATAATAAGAGAACTAGTTAATCAAAAAGGTTTAACTATTGATTGA", "aa": "MKEQSYFRIIADHFKAITFTISEGVLPGPNERNYVVRRLLRRALIACKKLQLNLAFIEKIIDEIIASYENYYQHLKAKNETVKQVVLKEINAFNKTIDLGLVLFEKSVKNNTLTPQLAFQLNETYGFPVEIIRELVNQKGLTID", "aa_hexdigest": "5c4cb1efa44df57df1e6ea4729139165", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16687.264799999986, "isoelectric_point": 9.161424827575683 }, "id": "JPCNHPFPBM_672", "locus": "GCA000292505_00657" }, { "type": "cds", "sequence": "contig_1", "start": 359886, "stop": 360068, "strand": "-", "frame": 2, "gene": null, "product": "alanine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920MQJ2" ], "nt": "GTGGGTTTTACTTCAAGACATCAAACTTTGTTTGAGATGCTTGGAAATTTTTCAATCGGTGATTATTTTAAAACAGAAGCAATTGATTTTGCTTTTGATCTTTTAGTTAATTATTATCAGCTAGATCCTAAGCGTTTTTATATCACTGTTTATGAAGATGATGAAACTACTTATAAAAGATGA", "aa": "MGFTSRHQTLFEMLGNFSIGDYFKTEAIDFAFDLLVNYYQLDPKRFYITVYEDDETTYKR", "aa_hexdigest": "7d6e2918c56b5049e14b9f42f329ec5e", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A920MQJ2", "query_cov": 0.9166666666666666, "subject_cov": 0.9649122807017544, "identity": 0.564, "score": 65.5, "evalue": 5.5e-13, "valid": false, "product": "alanine--tRNA ligase", "uniref50_id": "UniRef50_A0A920MQJ2" }, "pscc": { "uniref50_id": "UniRef50_A0A920MQJ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920MQJ2" ], "product": "alanine--tRNA ligase" }, "genes": [], "id": "JPCNHPFPBM_673", "locus": "GCA000292505_00658" }, { "type": "cds", "sequence": "contig_1", "start": 360514, "stop": 361089, "strand": "-", "frame": 1, "gene": null, "product": "putative MG293-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75367" ], "nt": "ATGCATAACAAGCAATTGCTTTTAGCACATAGGGGTTATTCATTCATTGCTCCAGAAAACACCAAACTAGCATTTGATTTAGCTTTTGAATATTGTTTTGATGGAATAGAGCTTGATGTTCATTTAACTAAAGATGAACAGTTAGTTATCATTCATGATGAGACAACATTGAGAACCGCATTAGTTAATAAGGAGGTTGAGTTTGAATCATTAGTTAGTTTAAAAAGAGATGATCATAGTGCTTTTTTTCACCTTAAAATTCAATTTCAATCGATCCTAACTTTAAAAGAGTTCTTAGATCTTTATTTAGATAAATTTAAGTTAATCAATATTGAGATTAAAACTGATCAAAAACCATATTTAGGAATTGAAAAGAAGCTTGTTGACCTAGTTAAAGGTTATGGTAAAAAAGCAATAGATAAGATCTTGTTTTCATCCTTTAACTTTGAATCTTTGCAAAAAGTTTATGATTTAGATAATAGTTACAAAAAAGGTTTTTTATTTTGGACTAAAAAACAGTTTGAAACAATTAGTACAGCTAGAATCCAAAAGATTTGTCAATTCCTCCACCCATGA", "aa": "MHNKQLLLAHRGYSFIAPENTKLAFDLAFEYCFDGIELDVHLTKDEQLVIIHDETTLRTALVNKEVEFESLVSLKRDDHSAFFHLKIQFQSILTLKEFLDLYLDKFKLINIEIKTDQKPYLGIEKKLVDLVKGYGKKAIDKILFSSFNFESLQKVYDLDNSYKKGFLFWTKKQFETISTARIQKICQFLHP", "aa_hexdigest": "052932c87959439b70799977b107973e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021153946", "query_cov": 1.0104712041884816, "subject_cov": 0.8041666666666667, "identity": 0.56, "score": 192.0, "evalue": 1.91e-58, "valid": false, "product": "glycerophosphodiester phosphodiesterase family protein", "uniref50_id": "UniRef50_P75367" }, "pscc": { "uniref50_id": "UniRef50_P75367", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75367" ], "product": "Uncharacterized protein MG293 homolog" }, "genes": [], "id": "JPCNHPFPBM_674", "locus": "GCA000292505_00659" }, { "type": "cds", "sequence": "contig_1", "start": 361103, "stop": 361285, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACTGCTGAAGTTGGTAAGAAATATACAGTTGCAGGACAAAGTAATACTAGTAACACTGGTTATCAAATTATCGCTGCTATTTGTTTATCCATTGCTTTGTTTGGTTTGATATGTGGATCAATTGTTTTTATCTCAAATAGTATGGAAATTAAACGATTAAATAAAGCTTCATACCGTTGA", "aa": "MTAEVGKKYTVAGQSNTSNTGYQIIAAICLSIALFGLICGSIVFISNSMEIKRLNKASYR", "aa_hexdigest": "cf0d1d4f4608e3693ae2dd3f8843f71b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6447.482499999996, "isoelectric_point": 9.445279121398926 }, "id": "JPCNHPFPBM_675", "locus": "GCA000292505_00660" }, { "type": "cds", "sequence": "contig_1", "start": 361295, "stop": 361726, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGATGTATGTGTTTCAAAGCACTTTTGCTTATTACCTTTTACAGATGTTGCAAAATGCATTTTTAGCACCTGTAGTATTAGTAACTGTAATTGGGGGAATTAGAACTTATGCACTGAGAAGTGCTGTTAGTGTTTATCTTGGTAGATTAGCAGATAAGTGTAAAAGTTATATCTTGTTTTTAATGATATGCACTGTTCTTGGTATTGTATTTGTTCTAGCATTTATTCTACTTGGTTTTGTACAAACAAATTCTGCTAACATCACTTTAATTACTTTCTCTTCCATTCTCTATATCTTTACTGGTATCTTATCATGGGGAATGGTTACAGTCAGATATAACCAAATAGGTGAAATTGATATTGGTAAAAATAACTATGCATCTAGTGTTGGTTTACTTAGTTTTATTGGTTTTTCTACTGATGGATGA", "aa": "MGMYVFQSTFAYYLLQMLQNAFLAPVVLVTVIGGIRTYALRSAVSVYLGRLADKCKSYILFLMICTVLGIVFVLAFILLGFVQTNSANITLITFSSILYIFTGILSWGMVTVRYNQIGEIDIGKNNYASSVGLLSFIGFSTDG", "aa_hexdigest": "363dafeb8c1311021ac0188a0209532b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15732.50129999998, "isoelectric_point": 8.975111198425292 }, "id": "JPCNHPFPBM_676", "locus": "GCA000292505_00661" }, { "type": "cds", "sequence": "contig_1", "start": 361754, "stop": 361972, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAATTACCAGCATTTTCTATCCATCAGGAGGTAGTGAGAATGCTGATTCAAAACCATTTGCTGCATACGCTTTTATCATTGCCATAATGCTAGTAATTACTGGATTTACAGTTTTGTTTTTTGTTAAAGAAAAGCCAATTAAAAAACAAAGTCAAACCACTTTAGTAAGTTTTAAACGCAATTTAAACCAGATTTTAGTGACCTTAAAAAACTGA", "aa": "MIITSIFYPSGGSENADSKPFAAYAFIIAIMLVITGFTVLFFVKEKPIKKQSQTTLVSFKRNLNQILVTLKN", "aa_hexdigest": "5ef9be8d3febea16d6d72ce3598de9a0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8044.470699999997, "isoelectric_point": 9.901779747009275 }, "id": "JPCNHPFPBM_677", "locus": "GCA000292505_00662" }, { "type": "cds", "sequence": "contig_1", "start": 362198, "stop": 362506, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATAGTGACAACAAAAAAAGGATTTAAAAAACGCTTAAAGGATTTTTCAAAACAACAAATTCTTGCCTTAATTATTTTGGGAGCAATTGATGTATTTGTAATTGCTGCACCCTATTATGTTAAGAATGTTGTTCCTAACCTCCACTTGTACTTAGGAATTACAGAGGATGAAGTTGCTACTGTTACTTCTATCATTGGTTATGTAACGCTTGCCACTCAACTACCAGGAGGGTTTTTAGCCAATCGCTTCAGTTCTAGAAAATTGTTATTTCTATCAGCAATTACTACTGGAGCAATTACTTCTTGA", "aa": "MIVTTKKGFKKRLKDFSKQQILALIILGAIDVFVIAAPYYVKNVVPNLHLYLGITEDEVATVTSIIGYVTLATQLPGGFLANRFSSRKLLFLSAITTGAITS", "aa_hexdigest": "486064f53d1b30e3074589ebb62e8669", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11118.077299999986, "isoelectric_point": 9.850978660583493 }, "id": "JPCNHPFPBM_678", "locus": "GCA000292505_00663" }, { "type": "cds", "sequence": "contig_1", "start": 362624, "stop": 362860, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF20258.4" ], "nt": "TTGAAAACAACAATTTTATTGGATCAATTTAACTGGTTGTATACACCAAAGCAACTTCCTAGTCAAGTTCTGGTAAGAATTAGACATGCTCAAAAACCAGAAATTGCAAAGTTGAAATTATTATCAGATAATAAACTGGAAATAACATTTAAAAATCCTGTTATAAGTGTTGCATCTGGACAGTTTGGTGTATTATATACACTTGATCAAATTTGTTTAGGAGCAGGATTAATTTAA", "aa": "MKTTILLDQFNWLYTPKQLPSQVLVRIRHAQKPEIAKLKLLSDNKLEITFKNPVISVASGQFGVLYTLDQICLGAGLI", "aa_hexdigest": "c1d2718396eccacb5089be333c7ea795", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF20258.4", "name": "tRNA_Me_trans_C", "length": 77, "aa_cov": 0.9743589743589743, "hmm_cov": 0.987012987012987, "evalue": 4.537314993488851e-20, "score": 66.9324722290039, "start": 3, "stop": 78 } ], "seq_stats": { "molecular_weight": 8781.335199999998, "isoelectric_point": 9.517612648010253 }, "id": "JPCNHPFPBM_679", "locus": "GCA000292505_00664" }, { "type": "cds", "sequence": "contig_1", "start": 363131, "stop": 363436, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03054.22" ], "nt": "ATGATTCAAAGTTTCAAAAAAGGGTTAACCCCAAATCCAGACATCTGGTGTAATCGTTTTATTAAGTTTGGTTTATTGCATGATTTTTGTAAGCAAATTAACCCTAATTCTCTTTTTGCAACTGGTCATTATGCCAAAATAAACATGATAGAAAATCAGCCTTTGCTTTCTATTCCTAAAGATACCAATAAAGATCAAACTTATTTTTTAGCAAATGTTAAAAAAGAACAATTTCAGAATGTTATTTTTCCTTTAGCAGATTTAAAAAAAATAACGGTGAGAAATATTGCTAGAGAAAATAATTGA", "aa": "MIQSFKKGLTPNPDIWCNRFIKFGLLHDFCKQINPNSLFATGHYAKINMIENQPLLSIPKDTNKDQTYFLANVKKEQFQNVIFPLADLKKITVRNIARENN", "aa_hexdigest": "5c07ac57deafb152956cd58897f2ad07", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03054.22", "name": "tRNA_Me_trans", "length": 102, "aa_cov": 0.9900990099009901, "hmm_cov": 0.9901960784313726, "evalue": 1.3650791118807828e-35, "score": 117.92342376708984, "start": 1, "stop": 100 } ], "seq_stats": { "molecular_weight": 11753.529599999983, "isoelectric_point": 9.554230690002441 }, "id": "JPCNHPFPBM_680", "locus": "GCA000292505_00665" }, { "type": "cds", "sequence": "contig_1", "start": 363469, "stop": 363726, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02540.23", "PFAM:PF03054.22" ], "nt": "ATGTCAATTATTGCAAAAACAGTTTTTATAGGTTTAAGTGGTGGTGTTGATTCTGCTGTTAGTGCTTTACTTTTAAAAAAGCAATACCAAGAAGTTATTGGTGTTTTTATGGAATGTTGGGATGAGACACTTAATAATGATTTTTATGGTCATAAGAAAATAAATAATAACAAATCAGGTTGTTCATCTTTTCAAGACTTCCAACAGGCTAAAAAAATCGCTAATTCTTTAGGAATTAAGTTAATAAAAAAAACTTAA", "aa": "MSIIAKTVFIGLSGGVDSAVSALLLKKQYQEVIGVFMECWDETLNNDFYGHKKINNNKSGCSSFQDFQQAKKIANSLGIKLIKKT", "aa_hexdigest": "0eab89fb9e4ee77a0df453c76d41615f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02540.23", "name": "NAD_synthase", "length": 36, "aa_cov": 0.4, "hmm_cov": 1.0, "evalue": 3.209634053869726e-08, "score": 28.064186096191406, "start": 5, "stop": 38 }, { "id": "PF03054.22", "name": "tRNA_Me_trans", "length": 77, "aa_cov": 0.9058823529411765, "hmm_cov": 0.8831168831168831, "evalue": 5.1285384732312894e-20, "score": 67.06993865966797, "start": 6, "stop": 82 } ], "seq_stats": { "molecular_weight": 9402.80389999999, "isoelectric_point": 9.200492668151856 }, "id": "JPCNHPFPBM_681", "locus": "GCA000292505_00666" }, { "type": "cds", "sequence": "contig_1", "start": 363729, "stop": 364118, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MG296", "db_xrefs": [ "RefSeq:WP_014894269.1", "SO:0001217", "UniParc:UPI00027B3BFF", "UniRef:UniRef100_UPI00027B3BFF", "UniRef:UniRef50_P75364", "UniRef:UniRef90_P47538" ], "nt": "ATGAAACCACAACTCATAGCTTTTAAAAAATTTTTACAAACTGAATTTCAAGCGGTTGATTTTGAGACTTTTCGCATTAACTTTAATTTGTGTTTAAAACGAGAACAAGACAATATTGTTATTTATGAAGATGATGATTATGATGACCAACCATTTTTTTTCAAACCAATGTTAAGTGATGGTTTTTTTATCCAAACTGAAGTAATTAAACAGCTAGATTATCTAGCAAAAGTTGTAGAAAATCCTAAAGATAGTGATCAGCAGTGCTGTCAAAATTTTTATGAAGCATTAATAGTGTTTATTTCTGCACTTGCTATTACAAAAGGCATTAATCCTAATAGATTTCACCAAAGATTAGTAAACAAGTTTGCTATTCACGCTGTTTATTAA", "aa": "MKPQLIAFKKFLQTEFQAVDFETFRINFNLCLKREQDNIVIYEDDDYDDQPFFFKPMLSDGFFIQTEVIKQLDYLAKVVENPKDSDQQCCQNFYEALIVFISALAITKGINPNRFHQRLVNKFAIHAVY", "aa_hexdigest": "3d5a4518cd1db8081b9d3c41233c775b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3BFF", "ncbi_nrp_id": "WP_014894269.1", "uniref100_id": "UniRef100_UPI00027B3BFF", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3BFF", "RefSeq:WP_014894269.1", "UniRef:UniRef100_UPI00027B3BFF" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3BFF", "uniref90_id": "UniRef90_P47538" }, "psc": { "uniref90_id": "UniRef90_P47538", "product": "Uncharacterized protein MG296", "uniref50_id": "UniRef50_P75364" }, "pscc": { "uniref50_id": "UniRef50_P75364", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75364" ], "product": "Uncharacterized protein MG296 homolog" }, "genes": [], "id": "JPCNHPFPBM_682", "locus": "GCA000292505_00667" }, { "type": "cds", "sequence": "contig_1", "start": 364238, "stop": 364570, "strand": "-", "frame": 3, "gene": null, "product": "Cell division protein FTSY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PYR8" ], "nt": "ATGAATGAATTGCAAAAAATTTATCAAATTATTCAAAAGGTAAGTGGAAGTGAACCTAGTGAAACACTTTTAGTTTTAGATGGTACAGTAGGTCAAACAGGTTTATCACAAGCAAAGGTATTTAATGAATTTTCCAAACTAACAGGGATTGTTTTAACTAAAATGGATGGTTCTGCTAAGGGTGGAATTATTTTAGCTATTAAAGATATGTTTAACCTGCCTGTTAAACTGATAGGTTTTGGTGAAAAAACTAGTGATTTAGCTATCTTTGATCTGGAAAAATATGTTTTAGGTTTACTTAATAACTTAAACTTAGATAATAAAGAAAATTAG", "aa": "MNELQKIYQIIQKVSGSEPSETLLVLDGTVGQTGLSQAKVFNEFSKLTGIVLTKMDGSAKGGIILAIKDMFNLPVKLIGFGEKTSDLAIFDLEKYVLGLLNNLNLDNKEN", "aa_hexdigest": "2c89b26b0ef6df32ef5a2a99fa59dd5e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PYR8", "query_cov": 0.9636363636363636, "subject_cov": 0.8346456692913385, "identity": 0.6509999999999999, "score": 139.0, "evalue": 2.35e-40, "valid": false, "product": "Cell division protein FTSY", "uniref50_id": "UniRef50_A0A3B0PYR8" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PYR8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PYR8" ], "product": "Cell division protein FTSY" }, "genes": [], "id": "JPCNHPFPBM_683", "locus": "GCA000292505_00668" }, { "type": "cds", "sequence": "contig_1", "start": 364721, "stop": 365278, "strand": "-", "frame": 3, "gene": "ftsY", "product": "signal recognition particle-docking protein FtsY", "db_xrefs": [ "COG:COG0552", "COG:U", "EC:3.6.5.4", "GO:0003924", "GO:0005047", "GO:0005525", "GO:0005737", "GO:0005886", "GO:0006605", "GO:0006614", "GO:0009306", "GO:0016887", "KEGG:K03110", "SO:0001217", "UniRef:UniRef50_P47539", "UniRef:UniRef90_P47539" ], "nt": "ATGGGCTTTTTAAGCAAGTTAATTGCAAAACTAAAACCAAAAAAATCAGTTGCTAAACAGCTTAAAGAAGAAGTTGAAAAACAAAGCCTTTTTCAAACCAATAATAAAACTTACTATCAGGGTTTGAAAAAATCTGCTACAACTTTCGCTAAAACTATTAATGAACTGTCAAAACGATATGTTAATGTTGACGAACAGTTTAAAGAAAATCTATTTGAAGGGTTAGTTTTGCTTGATGTTGGTTATCATGCTGCAAACAAAATTTGTGATGCTATTATTGAACAGATCAAGCTAAACAGAATTACAGATTTTCAACTCATTAAAGAGCTAATTATTGACCAAATCATTGTTTATTACATCCAAGATAAACTCTTTGATACTGATTTAATAGTTAAACCTAACTTTACAAATGTTTATCTCTTTGTTGGTGTTAATGGAGTTGGTAAAACAACTACTTTAGCTAAGATAGCGGATTTTTTCATAAAACAAAATAAACGTGTTCTACTTGTTGCAGGTGATACTTTTAGAGCAGGAGCCATTGAACAACTTAATCAGTGA", "aa": "MGFLSKLIAKLKPKKSVAKQLKEEVEKQSLFQTNNKTYYQGLKKSATTFAKTINELSKRYVNVDEQFKENLFEGLVLLDVGYHAANKICDAIIEQIKLNRITDFQLIKELIIDQIIVYYIQDKLFDTDLIVKPNFTNVYLFVGVNGVGKTTTLAKIADFFIKQNKRVLLVAGDTFRAGAIEQLNQ", "aa_hexdigest": "3ce5e31761c7177dd696a15c2d827d00", "start_type": "ATG", "rbs_motif": null, "genes": [ "ftsY" ], "truncated": "5-prime", "pseudogene": { "start": 364418, "stop": 365278, "observations": { "mutation": [ 364723 ], "stop": [ 364723 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47539", "query_cov": 1.0, "subject_cov": 0.5346820809248555, "identity": 1.0, "score": 350.0, "evalue": 2.8e-119, "gene_start": 1, "gene_end": 185, "reference_start": 1, "reference_end": 185, "reference_sequence": "MGFLSKLIAKLKPKKSVAKQLKEEVEKQSLFQTNNKTYYQGLKKSATTFAKTINELSKRYVNVDEQFKENLFEGLVLLDVGYHAANKICDAIIEQIKLNRITDFQLIKELIIDQIIVYYIQDKLFDTDLIVKPNFTNVYLFVGVNGVGKTTTLAKIADFFIKQNKRVLLVAGDTFRAGAIEQLNQWAKLLNCDIVLPNPKEQTPAVIFRGVKKGIDDKYDFVLCDTSGRLQNKLNLMNELQKIYQIIQKVSGSEPSETLLVLDGTVGQTGLSQAKVFNEFSKLTGIVLTKMDGSAKGGIILAIKDMFNLPVKLIGFGEKTSDLAIFDLEKYVLGLLNNLNLDNKEN" }, "paralog": false, "identity": 0.9895104895104895, "score": 551.0, "evalue": 3.66e-201, "description": "Internal stop codon at 364723. Nonsense mutation around 364723.", "psc": { "uniref90_id": "UniRef90_P47539", "gene": "ftsY", "product": "signal recognition particle-docking protein FtsY", "ec_ids": [ "3.6.5.4" ], "uniref50_id": "UniRef50_P47539", "cog_id": "COG0552", "cog_category": "U", "kegg_orthology_id": "K03110", "go_ids": [ "GO:0003924", "GO:0005047", "GO:0005525", "GO:0005737", "GO:0005886", "GO:0006605", "GO:0006614", "GO:0009306", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47539", "EC:3.6.5.4", "UniRef:UniRef50_P47539", "COG:COG0552", "COG:U", "KEGG:K03110", "GO:0003924", "GO:0005047", "GO:0005525", "GO:0005737", "GO:0005886", "GO:0006605", "GO:0006614", "GO:0009306", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47539", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47539" ], "product": "Signal recognition particle receptor FtsY" } }, "id": "JPCNHPFPBM_684", "locus": "GCA000292505_00669" }, { "type": "cds", "sequence": "contig_1", "start": 365285, "stop": 366121, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13304.12", "PFAM:PF02463.25" ], "nt": "TTGTTAGATGAACAACGTGCTAAATTAGTTTCAGCGCGGGAAGGAAAAATTAGGTTTGAAAATACAATCCAAAATATCACTGAAAAGATTAACAGTGTTTACAAAATGACAATGGAGTTTGCTATTGCTAATCACAACAAACCAGTTAAACTCTCTTCTATGCAAGCCCATAACAAGATTGCTAAACTACAAAATCAATTGAATGAAATGGGGGTAATTAACATGGAATCAATTGCTGAGATAAGTGAAAAGCAAAAGCGCTTTGATGATATTAACGCTGAATATGAATCAGCTCAACAAGCAGTTGAAAATTTGCAAAAAGCTATTACTGAAATTGATGAAATTGCTAGCAATGAGTTTGATCAACTGATCCAAAAATTAAATCAAGAGTTGCCAAAAACTTTCAAATATCTATTTGGTGGTGGTTCTTGCCAAATTCGTTACACTGATCCTAGTAATGTATTGGTTTCAGGGATAGATGTCTTTGCAAATCCTCCTGGTAAAAATATTGCCAACTTAATGTTGTTATCAGGCGGGGAAAAAACTTTAGTTGCACTTTCTGTTTTATTTAGTATTTTGAAAGTAAGTGCTTTTCCACTTGTTATTTTAGATGAAGCGGAAAGTGCACTTGATCCTGCTAATGTGGAGCGCTTTGCTAACATCATTAAAACTGCTAGCAAAAACACCCAATTTTTAATTATTACCCATCGTCAAGGTACAATGATGAAATGTGACATGTTATTGGGTGCTGCAATGCAAACTAAAGGTGTAACTAAAACCTTTGCAGTTGAACTTGAAAATGCTGAAAAATATGTATCTGAAAATGATTCTAATTAA", "aa": "MLDEQRAKLVSAREGKIRFENTIQNITEKINSVYKMTMEFAIANHNKPVKLSSMQAHNKIAKLQNQLNEMGVINMESIAEISEKQKRFDDINAEYESAQQAVENLQKAITEIDEIASNEFDQLIQKLNQELPKTFKYLFGGGSCQIRYTDPSNVLVSGIDVFANPPGKNIANLMLLSGGEKTLVALSVLFSILKVSAFPLVILDEAESALDPANVERFANIIKTASKNTQFLIITHRQGTMMKCDMLLGAAMQTKGVTKTFAVELENAEKYVSENDSN", "aa_hexdigest": "70422232939b4f45122da1d49838dcc0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13304.12", "name": "AAA_21", "length": 194, "aa_cov": 0.6942446043165468, "hmm_cov": 0.8247422680412371, "evalue": 2.662100025247147e-10, "score": 35.874908447265625, "start": 44, "stop": 236 }, { "id": "PF02463.25", "name": "SMC_N", "length": 198, "aa_cov": 0.7122302158273381, "hmm_cov": 0.5, "evalue": 1.7270547025598264e-29, "score": 97.88218688964844, "start": 61, "stop": 258 } ], "seq_stats": { "molecular_weight": 30970.167400000024, "isoelectric_point": 5.435876655578612 }, "id": "JPCNHPFPBM_685", "locus": "GCA000292505_00670" }, { "type": "cds", "sequence": "contig_1", "start": 366230, "stop": 366322, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATATGAACAGCTTACTAACACAACTTTTGATGGTAAAAAAGCTGTTGCTAGTGAAGCTGAATTAATCCATTCATTAAATAGTGCTTGA", "aa": "MEYEQLTNTTFDGKKAVASEAELIHSLNSA", "aa_hexdigest": "754aa796b3b07157b8b059fef832dfab", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3298.5878999999995, "isoelectric_point": 4.571128273010253 }, "id": "JPCNHPFPBM_686", "locus": "GCA000292505_00671" }, { "type": "cds", "sequence": "contig_1", "start": 366331, "stop": 367563, "strand": "-", "frame": 1, "gene": "smc", "product": "chromosome segregation protein SMC", "db_xrefs": [ "COG:COG1196", "COG:D", "GO:0003677", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0007062", "GO:0016887", "GO:0030261", "KEGG:K03529", "SO:0001217", "UniRef:UniRef50_P47540", "UniRef:UniRef90_P47540" ], "nt": "TTGAAAGAGTTGGAACTTGCTGTTTTGGTTGGGGAATATCTCCAAGCACAAACTGAACTTGATAAGTTTAATTTTCAGATTAACAGTAGTGAACATGACTTTAAAATACACGAACCCCAACTAGAGTTGTTAGAAGAACAGATTGTTATCTTTAATAGTCGCTTTCATAGTGCTGATATGCAATCTAATGAATTGCAAAAAGAGTTACAAGATATTTACCAAAAGATCAATGAATTGGAACAACGTAAAGTCATTATTGATGTGCAGTTAAGACAAGGTTTTTCACAAAAAGATGAAAAGCAAAAAGCTGCAGCTTTAAAAAAACTAATTTTAGTTGATCAAACCCAGCTTGATGGTTTTGAAAACCAACTATCAAATTCCAAAACAACCATCACTGATCTTGAAAAACTAATTAACGAACAAAAATCACTTGTTGATCAGATAAAGTTACAGATTGAAAAAAATACTGCTGATTTAATTTACCAACGTTCCTTAAAAACAATTATTGAACTCCAAACTAATGAGCTTAAAAAAACTAACAATGCCAATATCTTAGTTAAAAATGCTAATGCTTTAACAGGAATTTTAAATACACTAGGAACATTTCTTAAATTTGATAAGCAGTATGAAAAAGCTATCTTAAAAGCACTTGGAAAATCAATTGGTTACCTAGTTGTTAACAACAATAATGCTGCCATCCAAGCAATAGATTTTTTAGTCAAAAATGAGATAGGTAAAGTAACTTTTTTACCTTTAGATGATGTTGCTAGTGATACTAAAATCACAAACGAACACATGGAAATTTTAAAGCAGCTTGATGGTTTTCTTGGTGTTTGTAGTGATCATGTTAAGTGTGATCCATTGTTTCAACCAGTTGTAAATACTCTTTTAGCACAAGTTATTATTGCTAAAGATTTAAATAGTGCCATTAACCTTTCTAACTATACTTACAAGTTATATAGGATCGTTACTTTAGATGGTGAGACAGTTTATGCAGGGGGAATTATCAATGGTGGATTTGAAAAAACAAATCTTAGTGATGGGTATTTATCATCAGCTTCACTTGATAATGAACAGAACATTAACAAACTAGAAAACAACGAACGAGAGTTAAAAAAAGAGCTTACAGAATTAGAAGTAAAGCTAGATGAAATGAACAGAAAACTCAAGTATGAAGAGTTGTTACAAGCCAAGTTTATAGAGCGTATTGTTCAAATTAAAAAATTATTTTAG", "aa": "MKELELAVLVGEYLQAQTELDKFNFQINSSEHDFKIHEPQLELLEEQIVIFNSRFHSADMQSNELQKELQDIYQKINELEQRKVIIDVQLRQGFSQKDEKQKAAALKKLILVDQTQLDGFENQLSNSKTTITDLEKLINEQKSLVDQIKLQIEKNTADLIYQRSLKTIIELQTNELKKTNNANILVKNANALTGILNTLGTFLKFDKQYEKAILKALGKSIGYLVVNNNNAAIQAIDFLVKNEIGKVTFLPLDDVASDTKITNEHMEILKQLDGFLGVCSDHVKCDPLFQPVVNTLLAQVIIAKDLNSAINLSNYTYKLYRIVTLDGETVYAGGIINGGFEKTNLSDGYLSSASLDNEQNINKLENNERELKKELTELEVKLDEMNRKLKYEELLQAKFIERIVQIKKLF", "aa_hexdigest": "559e59710e0dbf379e83ad50cff660f4", "start_type": "TTG", "rbs_motif": null, "genes": [ "smc" ], "truncated": "both", "pseudogene": { "start": 366029, "stop": 367863, "observations": { "deletion": [ 366342 ], "mutation": [ 367785 ], "stop": [ 366231, 367785 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47540", "query_cov": 0.9975609756097561, "subject_cov": 0.4164969450101833, "identity": 0.995, "score": 699.0, "evalue": 5.16e-244, "gene_start": 1, "gene_end": 409, "reference_start": 224, "reference_end": 632, "reference_sequence": "MVFLKRFRAYGFKSYADEITIDFTHSMTGIVGPNGSGKSNVVDALKWVLGERSMKHLRSKSGDDMIFFGSKDKPASKLAEIELTFDNSNRLLHDSRKEISVMRRVYRGSGQSEYFINSNPATLKEISGIFADIGLEKGSLGIISQGSVSWFVEAKPEERRKIFEDASGIGRYTKRKEEVVNQLNRTLINLKQVSVVLNELKKDLKKLTLQAEKAQQFIRVKNELKELELAVLVGEYLQAQTELDKFNFQINSSEHDFKIHEPQLELLEEQIVIFNSRFHSADMQSNELQKELQDIYQKINELEQRKVIIDVQLRQGFSQKDEKQKAAALKKLILVDQTQLDGFENQLSNSKTTITDLEKLINEQKSLVDQIKLQIEKNTADLIYQRSLKTIIELQTNELKKTNNANILVKNANALTGILNTLGTFLKFDKQYEKAILKALGKSIGYLVVNNNNAAIQAIDFLVKNEIGKVTFLPLDDVASDTKITNEHMEILKQLDGFLGVCSDHVKCDPLFQPVVNTLLAQVIIAKDLNSAINLSNYTYKLYRIVTLDGETVYAGGIINGGFEKTNLSDGYLSSASLDNEQNINKLENNERELKKELTELEVKLDEMNRKLKYEELLQAKFIERIVQIKKIILELKMEYEQLTNTTFDGKKAVASEAELIHSLNSAWAKRDEINSKLKLNQELKLQLAKTIKQSEEKIVDLRALLDEQRAKLVSAREGKIRFENTIQNITEKINSVYKMTMEFAIANHNKPVKLSSMQAHNKIAKLQNQLNEMGVINMESIAEISEKQKRFDDINAEYESAQQAVENLQKAITEIDEIASNEFDQLIQKLNQELPKTFKYLFGGGSCQIRYTDPSNVLVSGIDVFANPPGKNIANLMLLSGGEKTLVALSVLFSILKVSAFPLVILDEAESALDPANVERFANIIKTASKNTQFLIITHRQGTMMKCDMLLGAAMQTKGVTKTFAVELENAEKYVSENDSN" }, "paralog": false, "identity": 0.9967266775777414, "score": 1083.0, "evalue": 0.0, "description": "Internal stop codon at 366231, 367785. Frameshift due to deletion around 366342. Nonsense mutation around 367785.", "psc": { "uniref90_id": "UniRef90_P47540", "gene": "smc", "product": "chromosome segregation protein SMC", "uniref50_id": "UniRef50_P47540", "cog_id": "COG1196", "cog_category": "D", "kegg_orthology_id": "K03529", "go_ids": [ "GO:0003677", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0007062", "GO:0016887", "GO:0030261" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47540", "UniRef:UniRef50_P47540", "COG:COG1196", "COG:D", "KEGG:K03529", "GO:0003677", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006260", "GO:0007059", "GO:0007062", "GO:0016887", "GO:0030261" ] }, "pscc": { "uniref50_id": "UniRef50_P47540", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47540" ], "product": "Chromosome partition protein Smc" } }, "id": "JPCNHPFPBM_687", "locus": "GCA000292505_00672" }, { "type": "cds", "sequence": "contig_1", "start": 367580, "stop": 367918, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTGTTGTGCTTTTTCAGCTTGTAATGTAAGCTTTTTTAAATCTTTTTTCAGTTCATTTAAAACAACGCTAACTTGTTTTAAGTTAATTAAGGTTCGATTTAACTGGTTAACAACTTCTTCTTTACGCTTGGTATATCTACCAATTCCCGACGCATCTTCAAAGATCTTTCTGCGTTCTTCTGGCTTAGCTTCTACAAATCAAGAGACACTCCCTTGTGAAATAATTCCAAGTGAACCTTTTTCAAGACCAATATCAGCAAAAATACCGCTAATTTCTTTTAAAGTTGCTGGATTGGAGTTAATAAAATATTCACTCTGGCCACTCCCTCTATAA", "aa": "MNCCAFSACNVSFFKSFFSSFKTTLTCFKLIKVRFNWLTTSSLRLVYLPIPDASSKIFLRSSGLASTNQETLPCEIIPSEPFSRPISAKIPLISFKVAGLELIKYSLWPLPL", "aa_hexdigest": "e6ec10abafac792283f12dc0d48fefe9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12563.762599999984, "isoelectric_point": 9.46655368804932 }, "id": "JPCNHPFPBM_688", "locus": "GCA000292505_00673" }, { "type": "cds", "sequence": "contig_1", "start": 367783, "stop": 368232, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13175.12", "PFAM:PF13304.12", "PFAM:PF13476.12", "PFAM:PF13555.12", "PFAM:PF02463.25" ], "nt": "ATGGTTTTTCTAAAACGTTTTCGCGCATATGGTTTTAAATCATATGCTGATGAAATCACAATTGATTTTACCCATTCAATGACTGGTATTGTTGGTCCTAATGGATCGGGCAAATCCAATGTTGTTGATGCATTAAAGTGGGTTTTAGGGGAGAGAAGTATGAAACATCTCCGTAGTAAATCAGGTGATGATATGATCTTTTTTGGCTCTAAAGACAAACCTGCTAGTAAGTTAGCTGAAATAGAGCTAACCTTTGATAATTCCAACCGTTTATTACATGATTCAAGAAAAGAGATAAGCGTGATGCGTAGGGTTTATAGAGGGAGTGGCCAGAGTGAATATTTTATTAACTCCAATCCAGCAACTTTAAAAGAAATTAGCGGTATTTTTGCTGATATTGGTCTTGAAAAAGGTTCACTTGGAATTATTTCACAAGGGAGTGTCTCTTGA", "aa": "MVFLKRFRAYGFKSYADEITIDFTHSMTGIVGPNGSGKSNVVDALKWVLGERSMKHLRSKSGDDMIFFGSKDKPASKLAEIELTFDNSNRLLHDSRKEISVMRRVYRGSGQSEYFINSNPATLKEISGIFADIGLEKGSLGIISQGSVS", "aa_hexdigest": "17ea2e276da776f1adc2da1b3e150dee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13175.12", "name": "AAA_15", "length": 83, "aa_cov": 0.5503355704697986, "hmm_cov": 0.9879518072289156, "evalue": 1.1149086989916224e-11, "score": 40.01136016845703, "start": 11, "stop": 92 }, { "id": "PF13304.12", "name": "AAA_21", "length": 28, "aa_cov": 0.18791946308724833, "hmm_cov": 0.9285714285714286, "evalue": 3.2960496374500346e-11, "score": 38.8541374206543, "start": 27, "stop": 54 }, { "id": "PF13476.12", "name": "AAA_23", "length": 120, "aa_cov": 0.7785234899328859, "hmm_cov": 0.9333333333333333, "evalue": 1.8746761264763956e-18, "score": 62.935909271240234, "start": 11, "stop": 126 }, { "id": "PF13555.12", "name": "AAA_29", "length": 37, "aa_cov": 0.24161073825503357, "hmm_cov": 0.972972972972973, "evalue": 2.1784597061029978e-08, "score": 28.913469314575195, "start": 14, "stop": 49 }, { "id": "PF02463.25", "name": "SMC_N", "length": 137, "aa_cov": 0.912751677852349, "hmm_cov": 0.9854014598540146, "evalue": 8.696539449566303e-42, "score": 138.0916290283203, "start": 3, "stop": 138 } ], "seq_stats": { "molecular_weight": 16489.59029999998, "isoelectric_point": 9.360503196716309 }, "id": "JPCNHPFPBM_689", "locus": "GCA000292505_00674" }, { "type": "cds", "sequence": "contig_1", "start": 368639, "stop": 369292, "strand": "-", "frame": 3, "gene": "pta", "product": "phosphate acetyltransferase", "db_xrefs": [ "COG:C", "COG:COG0280", "EC:2.3.1.8", "GO:0005737", "GO:0006085", "GO:0006086", "GO:0008959", "SO:0001217", "UniRef:UniRef50_P47541", "UniRef:UniRef90_P47541" ], "nt": "ATGCTTAATGAATCTAAGCTGATCCAACCTGCAGTTATCTTTCATAATCGTCAGGAAATCCCTGCAAATTTTGATAAAAAAATAACTCATTATGTGATTGATGAGATGGATTTAACTAGCTATGCTAACTTTGTCTATGAAAAACGTAAGCATAAGGGGATGGATTTAAAAGAAGCACAAAAGTTTGTACGTGATCCTAGTTCTTTAGCTGCTACCTTAGTTGCTCTAAAGGTTGTTGATGGTGAGGTTTGTGGTAAAGAATATGCTACAAAAGATACTTTAAGACCAGCTTTACAGTTACTAGCAACTGGTAATTTTGTTTCTAGTGTTTTCATCATGGAAAAAGGTGAAGAACGTTTGTACTTCACTGATTGTGCTTTTGCTGTTTATCCTAACCCCCAAGAGTTAGCAACAATTGCTGAAAACACCTTCAATTTTGCCAAAAGTTTAAATGAGGATGAGATAAAAATGGCTTTTTTAAGCTATTCAACGCTTGGCAGTGGTAAGGGTGAAATGGTGGATAAAGTTGTTTTAGCAACTAAACTATTTTTAGAAAAACACCCTGAATTGCATCAAAGTGTTTGTGGTGAGCTCCAGTTTGATGCTGCTTTTGTTGAAAAGGTTAGGTTACAAAAAGCACCTCAACTAACTTGA", "aa": "MLNESKLIQPAVIFHNRQEIPANFDKKITHYVIDEMDLTSYANFVYEKRKHKGMDLKEAQKFVRDPSSLAATLVALKVVDGEVCGKEYATKDTLRPALQLLATGNFVSSVFIMEKGEERLYFTDCAFAVYPNPQELATIAENTFNFAKSLNEDEIKMAFLSYSTLGSGKGEMVDKVVLATKLFLEKHPELHQSVCGELQFDAAFVEKVRLQKAPQLT", "aa_hexdigest": "a2508276b08614e1a06e8e22a53652c7", "start_type": "ATG", "rbs_motif": null, "genes": [ "pta" ], "truncated": "both", "pseudogene": { "start": 368435, "stop": 369397, "observations": { "mutation": [ 368641, 369322 ], "stop": [ 368641, 369322 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47541", "query_cov": 1.0, "subject_cov": 0.678125, "identity": 0.995, "score": 427.0, "evalue": 1.56e-149, "gene_start": 1, "gene_end": 217, "reference_start": 36, "reference_end": 252, "reference_sequence": "MSVIDIFKKRLQAVSKKPVIIFPEGWSASVLKAVEMLNESKLIQPAVIFHNRQEIPANFDKKITHYVIDEMDLTSYANFVYEKRKHKGMDLKEAQKFVRDPSSLAATLVALKVVDGEVCGKEYATKDTLRPALQLLATGNFVSSVFIMEKGEERLYFTDCAFAVYPNSQELATIAENTFNFAKSLNEDEIKMAFLSYSTLGSGKGEMVDKVVLATKLFLEKHPELHQSVCGELQFDAAFVEKVRLQKAPQLTWKNSANIYVFPNLDAGNIAYKIAQRLGGYDAIGPIVLGLSSPVNDLSRGASVSDIFNVGIITAAQAIK" }, "paralog": false, "identity": 0.990625, "score": 613.0, "evalue": 1.05e-225, "description": "Internal stop codon at 368641, 369322. Nonsense mutation around 368641, 369322.", "psc": { "uniref90_id": "UniRef90_P47541", "gene": "pta", "product": "phosphate acetyltransferase", "ec_ids": [ "2.3.1.8" ], "uniref50_id": "UniRef50_P47541", "cog_id": "COG0280", "cog_category": "C", "go_ids": [ "GO:0005737", "GO:0006085", "GO:0006086", "GO:0008959" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47541", "EC:2.3.1.8", "UniRef:UniRef50_P47541", "COG:COG0280", "COG:C", "GO:0005737", "GO:0006085", "GO:0006086", "GO:0008959" ] }, "pscc": { "uniref50_id": "UniRef50_P47541", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47541" ], "product": "Phosphate acetyltransferase" } }, "id": "JPCNHPFPBM_690", "locus": "GCA000292505_00675" }, { "type": "cds", "sequence": "contig_1", "start": 369415, "stop": 369525, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACTATCTGATCAGTTTAGTTTTATCTCCACTGGTGGTGGTGCTTCTTTAGCACTAATTGGTGGGGAAGAGTTAGTAGGTATTAGCGATATTCAAAAAAATTCTTAA", "aa": "MQLSDQFSFISTGGGASLALIGGEELVGISDIQKNS", "aa_hexdigest": "72e6128bc3450b00e01137ee446d32a7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3671.0473, "isoelectric_point": 4.0500284194946286 }, "id": "JPCNHPFPBM_691", "locus": "GCA000292505_00676" }, { "type": "cds", "sequence": "contig_1", "start": 369658, "stop": 370650, "strand": "-", "frame": 1, "gene": "pgk", "product": "Phosphoglycerate kinase", "db_xrefs": [ "COG:COG0126", "COG:G", "EC:2.7.2.3", "GO:0004618", "GO:0005524", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0043531", "KEGG:K00927", "SO:0001217", "UniRef:UniRef50_Q7WTU1", "UniRef:UniRef90_P47542" ], "nt": "ATGCTTAATTTCAAAACACTCCAAGCAATTGATTTTCAAAACAAAACCGTTGTTTTAAGAAGTGATTTTAATGTCCCAATGATCAATGGGGTTATTAGTGATAGTGAAAGAATTTTAGCTGGTTTGGATACTATTAAGTTCTTAGTTAAAAAGAACTGCAAGATAGTGCTACTATCACACCTTTCAAGGATTAAGAGTTTAGAAGATAAACTAAACAACAAAAAATCTTTAAAGCCAGTTGCTGAATTACTCCAACAACTCTTACCAACTGTAAAGGTTCAATTTTCTTGTAAAAACACTGGTGCTGAAGTTAAACAAAAAGTGCAAGCATTAGCATTCGGTGAAATCCTTCTCCTTGAAAACACTCGCTATTGTGATGTAAACGATAAAGGAGAAATTGTTAAATTAGAAAGTAAAAATGATCCTGAACTAGCGAAATTCTGGGCTAGTTTAGGGGAAATTTTTGTTAATGATGCATTTGGTACTGCCCATAGAAAACATGCTTCTAATGCAGGAATTGCAAAGTATGTTGCAAAATCCTGTATTGGGTTTTTAATGGAAAAAGAACTAAAGAACCTCTCTTACCTAATTCAAAGCCCACAAAAACCCTTTGTTGTTGTTTTGGGTGGTGCGAAAGTATCAGATAAACTAAAGGTAGTTGAAAACTTACTAAAACTTGCTGATAATATCTTAATTGGCGGAGGGATGGTAAATACCTTTCTTAAAGCAAAAGGCAAAGCTACTGCTAATTCCCTAGTTGAAAAAGAGTTAATTGATGTTGCTAAGCAAATCTTGGATAAAGATACTCATAATAAGATTGTGCTGGCAATTGATCAGGTAATGGGTTCTGAATTTAAAGATCAAGCTGGCATTACTTTAGATGTTAGTGACAAAATTCAAGAACAATATCAATCCTATATGTCTCTAGATGTTGGATCTAAAACAATTGCTTTATTTGAAAGTTATTTAAAAACAGCCAAAACTATCTTTTGA", "aa": "MLNFKTLQAIDFQNKTVVLRSDFNVPMINGVISDSERILAGLDTIKFLVKKNCKIVLLSHLSRIKSLEDKLNNKKSLKPVAELLQQLLPTVKVQFSCKNTGAEVKQKVQALAFGEILLLENTRYCDVNDKGEIVKLESKNDPELAKFWASLGEIFVNDAFGTAHRKHASNAGIAKYVAKSCIGFLMEKELKNLSYLIQSPQKPFVVVLGGAKVSDKLKVVENLLKLADNILIGGGMVNTFLKAKGKATANSLVEKELIDVAKQILDKDTHNKIVLAIDQVMGSEFKDQAGITLDVSDKIQEQYQSYMSLDVGSKTIALFESYLKTAKTIF", "aa_hexdigest": "bc68450ef6a465d6f7aba9b0158d1a58", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47542", "query_cov": 1.0, "subject_cov": 0.8029197080291971, "identity": 0.997, "score": 631.0, "evalue": 7.77e-227, "valid": true, "gene": "pgk", "product": "Phosphoglycerate kinase", "ec_ids": [ "2.7.2.3" ], "uniref50_id": "UniRef50_Q7WTU1", "cog_id": "COG0126", "cog_category": "G", "kegg_orthology_id": "K00927", "go_ids": [ "GO:0005829", "GO:0043531", "GO:0005524", "GO:0004618", "GO:0006094", "GO:0006096" ] }, "pscc": { "uniref50_id": "UniRef50_Q7WTU1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q7WTU1" ], "product": "Phosphoglycerate kinase" }, "genes": [ "pgk" ], "id": "JPCNHPFPBM_692", "locus": "GCA000292505_00677" }, { "type": "cds", "sequence": "contig_1", "start": 370652, "stop": 371665, "strand": "-", "frame": 3, "gene": "gap", "product": "type I glyceraldehyde-3-phosphate dehydrogenase", "db_xrefs": [ "COG:COG0057", "COG:G", "EC:1.2.1.12", "GO:0004365", "GO:0005737", "GO:0006006", "GO:0006096", "GO:0006735", "GO:0009986", "GO:0016620", "GO:0044650", "GO:0050661", "GO:0051287", "GO:0140032", "RefSeq:WP_009885879.1", "SO:0001217", "UniParc:UPI000012AEC4", "UniRef:UniRef100_P47543", "UniRef:UniRef50_P47543", "UniRef:UniRef90_P47543" ], "nt": "ATGGCAGCAAAGAATAGAACCATTAAGGTTGCAATCAATGGTTTTGGAAGAATTGGAAGACTTGTTTTTCGTTCTCTTCTCAGTAAGGCAAATGTTGAAGTTGTAGCAATTAATGATTTGACCCAACCTGAAGTTTTAGCGCACCTGTTGAAATATGATTCAGCTCATGGTGAATTGAAAAGAAAGATTACTGTTAAACAAAACATCTTGCAAATTGATAGAAAAAAGGTTTATGTTTTTAGTGAAAAAGATCCCCAAAATTTACCTTGGGATGAACATGATATTGATGTAGTAATTGAATCAACTGGTAGGTTTGTAAGTGAAGAGGGTGCTTCTCTCCATTTAAAAGCAGGTGCTAAAAGAGTAATTATTTCCGCACCCGCTAAAGAAAAAACTATCAGGACAGTTGTTTACAATGTTAATCACAAAACCATTAGTAGCGATGATAAGATCATCTCAGCAGCTAGCTGTACTACTAACTGTTTAGCACCATTAGTTCATGTACTTGAAAAGAACTTTGGGATTGTTTATGGAACGATGCTAACAGTTCATGCATATACTGCAGATCAACGCTTACAAGATGCTCCTCATAATGACTTACGTCGTGCTCGTGCTGCAGCTGTTAACATTGTGCCAACAACAACAGGAGCAGCTAAAGCAATTGGGCTTGTTGTTCCAGAAGCAAATGGCAAACTTAATGGGATGTCACTCCGTGTTCCAGTGTTAACTGGTTCTATTGTAGAGTTAAGTGTTGTACTTGAAAAAAGTCCATCTGTTGAACAAGTAAATCAAGCCATGAAGCGATTTGCTTCCGCTTCTTTTAAATATTGTGAAGATCCTATTGTATCTAGCGATGTGGTAAGTTCTGAATATGGTTCAATTTTCGATTCTAAACTAACCAATATTGTTGAAGTTGATGGCATGAAACTTTATAAGGTGTATGCATGGTATGATAATGAATCTTCCTATGTACACCAACTAGTGAGAGTAGTTAGCTATTGTGCTAAGCTCTAA", "aa": "MAAKNRTIKVAINGFGRIGRLVFRSLLSKANVEVVAINDLTQPEVLAHLLKYDSAHGELKRKITVKQNILQIDRKKVYVFSEKDPQNLPWDEHDIDVVIESTGRFVSEEGASLHLKAGAKRVIISAPAKEKTIRTVVYNVNHKTISSDDKIISAASCTTNCLAPLVHVLEKNFGIVYGTMLTVHAYTADQRLQDAPHNDLRRARAAAVNIVPTTTGAAKAIGLVVPEANGKLNGMSLRVPVLTGSIVELSVVLEKSPSVEQVNQAMKRFASASFKYCEDPIVSSDVVSSEYGSIFDSKLTNIVEVDGMKLYKVYAWYDNESSYVHQLVRVVSYCAKL", "aa_hexdigest": "01db5001e72ea8a645150eda93526624", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012AEC4", "ncbi_nrp_id": "WP_009885879.1", "uniref100_id": "UniRef100_P47543", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012AEC4", "RefSeq:WP_009885879.1", "UniRef:UniRef100_P47543" ] }, "ips": { "uniref100_id": "UniRef100_P47543", "uniref90_id": "UniRef90_P47543" }, "psc": { "uniref90_id": "UniRef90_P47543", "gene": "gap", "product": "type I glyceraldehyde-3-phosphate dehydrogenase", "ec_ids": [ "1.2.1.12" ], "uniref50_id": "UniRef50_P47543", "cog_id": "COG0057", "cog_category": "G", "go_ids": [ "GO:0004365", "GO:0005737", "GO:0006006", "GO:0006096", "GO:0006735", "GO:0009986", "GO:0016620", "GO:0044650", "GO:0050661", "GO:0051287", "GO:0140032" ] }, "pscc": { "uniref50_id": "UniRef50_P47543", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47543" ], "product": "Glyceraldehyde-3-phosphate dehydrogenase" }, "genes": [ "gap" ], "id": "JPCNHPFPBM_693", "locus": "GCA000292505_00678" }, { "type": "cds", "sequence": "contig_1", "start": 371810, "stop": 372142, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGCAGTTATATTTAAATTACTCCCAACTGTTAAAAGTGAAATTATAAGAATTAAACAAGCTCAAGCTACTAGAGGTTTTATCTATAACAAGTGTTCGTTTTTAAATCCTTTCAAGATTAAAACCCTTTTTATTCCCGTATTACTTTCAACAGTTAAAAAAACAGAAACAACAGCGTTTGCTCTACAAGCTAAAGGTTATGATTTAAATAACACTAACAGAACTCATTATCCTTTAAAATACAACTTATTAAACGGGGTATTTTTATTAGTGGGTTTACTTTTATTTAGTATTTTATTAATTGCTAATAACTGGAATTTAGTTTACTGA", "aa": "MLAVIFKLLPTVKSEIIRIKQAQATRGFIYNKCSFLNPFKIKTLFIPVLLSTVKKTETTAFALQAKGYDLNNTNRTHYPLKYNLLNGVFLLVGLLLFSILLIANNWNLVY", "aa_hexdigest": "49bee9ae139ee3bd1b583fedbdb8e75d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12565.911899999983, "isoelectric_point": 10.063337516784667 }, "id": "JPCNHPFPBM_694", "locus": "GCA000292505_00679" }, { "type": "cds", "sequence": "contig_1", "start": 372320, "stop": 372463, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTATTTTTCTTGTGGTTTAATGTAATTGTTAACGGTTTTATCTTTTTACCAAATACTGCTTTAAGCGTTGATCAGAACCATAATTTTTTAGGAAGTTTTATCTATTCAGGTGGTAATAATTTTGGTGGTGTTAGCTGGTGA", "aa": "MLFFLWFNVIVNGFIFLPNTALSVDQNHNFLGSFIYSGGNNFGGVSW", "aa_hexdigest": "a1e6d754f68a9901808a4d6829d752be", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5302.9676, "isoelectric_point": 5.060625648498534 }, "id": "JPCNHPFPBM_695", "locus": "GCA000292505_00680" }, { "type": "cds", "sequence": "contig_1", "start": 372628, "stop": 373701, "strand": "-", "frame": 1, "gene": "ecfA2", "product": "Putative ABC transporter ATP-binding protein MG303", "db_xrefs": [ "COG:COG1122", "COG:PR", "GO:0005524", "GO:0016887", "GO:0042626", "GO:0043190", "RefSeq:WP_010869419.1", "SO:0001217", "UniParc:UPI00001395D4", "UniRef:UniRef100_P47545", "UniRef:UniRef50_P75356", "UniRef:UniRef90_P47545" ], "nt": "ATGCAAACACCTCAAATAAAACTAGATTTAATTGAACAAAAATACCATCTTCAAGCTCAAAAAGCGAATCTTAAACTAGCAAAATTAGATTCAAAAAAACTGTTTTCTTTTTATAAAAAGGTTGATAAAATCATTAATCCTTTTTACTTTTTAAACAGTAAAAAAAGCATTCCATTTTTTAATAGCAAATTACTGAACATTGAACAAGATCCATTGTTTTTTAACAATATTAGTGTCTTTGTCAATAAGCATAATGATGGTCAGATCATTAAATGTTGTTCAGGGGTAATAGAACCTAATAAAATTACAGTTATTTTTGGTGAAAGTGGTAGTGGTAAAACTACACTAATAAAACAGTTAGGTTTATTTGAAAAACCTAGTTTTGGCTATATAAATTGTGCTAATTATTGTTATTTTGCAAACCAATACCAACAAAAGATAACAAAAAATTTTAAGCAAAAAATTGGTTATATTTTGCAAAAAGCAGAAGATCAGTTTTTTTGTGATTCTATTTTAGAAGAAGTTTTAACAGGAGCAGTGAACCTTAAACTTTGTCATAAAAAAGATGTTAATTATGCCAAAAAATACCTTGATCTATGTGGATTGGAAAACATCCCGTTAATAAAAAATCCCATTGAATTAAGCGATGGACAGAAGAAGCGTTTGGCATTAGCTAGTGTGTTAGCAATGCAAGTTAAGTTTTTAATCCTTGATGAACCTACTGTTGGGTTAGATCAAATGGCGATTAGCAACTTAAGCAAAATGCTAGTAGCAATGAAACAAACAACAAGGATTGTTATTGTTAGCCATGATGTTGATTTTATCTTTGAAACTGCTGATAAAATCATCCATCTCCACCAGGGAAAGATAATTCATCAAACCACTGTTAATGATTTTTTTTCAAACACCAGTTGGTTGATGCAATATGGAATCACCCCTCCAGTAATTATCCAAGCAGTTAAGATGTTTAATGAAAAGGGAATAGCTTTTAAAAACCAAGCAGAAATTAAAAACCTTGATGATCTTATTAGTGAACTAAAAAACTTATTTTCATGCAAAAAACCAGTTTTTTAA", "aa": "MQTPQIKLDLIEQKYHLQAQKANLKLAKLDSKKLFSFYKKVDKIINPFYFLNSKKSIPFFNSKLLNIEQDPLFFNNISVFVNKHNDGQIIKCCSGVIEPNKITVIFGESGSGKTTLIKQLGLFEKPSFGYINCANYCYFANQYQQKITKNFKQKIGYILQKAEDQFFCDSILEEVLTGAVNLKLCHKKDVNYAKKYLDLCGLENIPLIKNPIELSDGQKKRLALASVLAMQVKFLILDEPTVGLDQMAISNLSKMLVAMKQTTRIVIVSHDVDFIFETADKIIHLHQGKIIHQTTVNDFFSNTSWLMQYGITPPVIIQAVKMFNEKGIAFKNQAEIKNLDDLISELKNLFSCKKPVF", "aa_hexdigest": "c997a1eeb2e0f5acd2b08de7ef39d05a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001395D4", "ncbi_nrp_id": "WP_010869419.1", "uniref100_id": "UniRef100_P47545", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001395D4", "RefSeq:WP_010869419.1", "UniRef:UniRef100_P47545" ] }, "ips": { "uniref100_id": "UniRef100_P47545", "uniref90_id": "UniRef90_P47545" }, "psc": { "uniref90_id": "UniRef90_P47545", "gene": "EcfA2", "product": "Putative ABC transporter ATP-binding protein MG303", "uniref50_id": "UniRef50_P75356", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_P75356", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75356" ], "product": "Putative ABC transporter ATP-binding protein MG303 homolog" }, "genes": [ "ecfA2" ], "id": "JPCNHPFPBM_696", "locus": "GCA000292505_00681" }, { "type": "cds", "sequence": "contig_1", "start": 373683, "stop": 374495, "strand": "-", "frame": 2, "gene": "ecfA2", "product": "Putative ABC transporter ATP-binding protein MG304", "db_xrefs": [ "COG:COG1122", "COG:PR", "GO:0005524", "GO:0016887", "GO:0042626", "GO:0043190", "RefSeq:WP_009885882.1", "SO:0001217", "UniParc:UPI0000557DFF", "UniRef:UniRef100_P47546", "UniRef:UniRef50_P47546", "UniRef:UniRef90_P47546" ], "nt": "TTGCTACAAGTTAAAAACCTCAGTTTTAAATACCCAAAACACCAAAACAATGTGTTAAATCAGATTAGTTTTAATGTTCAAGATGGGTGTCATTTAGCAGTAATAGGTCATAACGGTTCAGGTAAATCAACCCTAGTTAAACTCCTTGGCGGATTTCTAAAAGCTAAAAAAGGAACTATCTTTTTCAATGATAAGGAGTTGACTTCTGTTGGTTTTAATAAGATTGGAATCTTATTACAAGACCCTGATGTGCAATTACTTGCTGATACACTCCACCAGGAATTGATTTTTACTTTGGAAAACCATGGAGTTTTAGCTAGTGAAATGGATAAGATTATTAATGAAGTTTTAACTGTTGTTGAATTGAAAGATAAACAATTTACACCACTTAAAAAACTTTCATTTGGTGAAAAACAACGTGCTGTTTTTGCTTGTTTATTGGCTGTCAAACCACAAATTTATCTGCTTGATGAGGCATTTAGTATGCTTGATAATAAGATCAGCAATAAACTTAAAAAATTTGTGTTTGATACCATCAAAAAACAAAACAAAATAGTTATCAATATTACCCATGATTTTAATGATCTGTTTCTAGCAGATGAGATTATCTTTTTAAGTAAAGGATCGCTAATTAAAAAGTTTGCTCCTGAAGCGATATATGAGCAGCTTGATTTGTTTCATAACCACCACTTTAATCTCCCTTTTCCACTTTTATTGGCCCATAAGGTGGCTAAACAAATAAACAAAAAAACACCAAGTTTAAGTTTTGAATTGAATGATGTAGTTAACTGGATATGCAAACACCTCAAATAA", "aa": "MLQVKNLSFKYPKHQNNVLNQISFNVQDGCHLAVIGHNGSGKSTLVKLLGGFLKAKKGTIFFNDKELTSVGFNKIGILLQDPDVQLLADTLHQELIFTLENHGVLASEMDKIINEVLTVVELKDKQFTPLKKLSFGEKQRAVFACLLAVKPQIYLLDEAFSMLDNKISNKLKKFVFDTIKKQNKIVINITHDFNDLFLADEIIFLSKGSLIKKFAPEAIYEQLDLFHNHHFNLPFPLLLAHKVAKQINKKTPSLSFELNDVVNWICKHLK", "aa_hexdigest": "4d033138b35b5fe289f313b2d8a63aef", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000557DFF", "ncbi_nrp_id": "WP_009885882.1", "uniref100_id": "UniRef100_P47546", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000557DFF", "RefSeq:WP_009885882.1", "UniRef:UniRef100_P47546" ] }, "ips": { "uniref100_id": "UniRef100_P47546", "uniref90_id": "UniRef90_P47546" }, "psc": { "uniref90_id": "UniRef90_P47546", "gene": "EcfA2", "product": "Putative ABC transporter ATP-binding protein MG304", "uniref50_id": "UniRef50_P47546", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0043190", "GO:0005524", "GO:0016887", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_P47546", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47546" ], "product": "Putative ABC transporter ATP-binding protein MG304" }, "genes": [ "ecfA2" ], "id": "JPCNHPFPBM_697", "locus": "GCA000292505_00682" }, { "type": "cds", "sequence": "contig_1", "start": 374681, "stop": 376408, "strand": "-", "frame": 3, "gene": "dnaK", "product": "molecular chaperone DnaK", "db_xrefs": [ "COG:COG0443", "COG:O", "GO:0005524", "GO:0006457", "GO:0051082", "GO:0140662", "SO:0001217", "UniRef:UniRef50_P9WMJ9", "UniRef:UniRef90_P47547" ], "nt": "ATGAGTGCAGACAATGGTTTAATTATTGGCATTGACCTTGGAACTACCAATTCTTGTGTTTCTGTAATGGAAGGTGGGAGACCTGTTGTATTAGAAAATCCTGAAGGTAAAAGAACAACACCTTCCATTGTTTCCTATAAAAACAATGAAATTATAGTAGGTGATGCTGCTAAAAGACAGATGGTTACAAACCCAAATACCATTGTCTCCATCAAGAGGTTGATGGGTACCTCAAATAAAGTAAAAGTCCAAAATGCTGATGGTACAACTAAGGAATTAAGTCCTGAACAAGTTTCAGCGCAAATCCTTAGTTATCTTAAGGACTTTGCTGAAAAAAAGATTGGTAAAAAGATTTCAAGAGCAGTTATTACTGTTCCTGCATACTTTAATGATGCGGAAAGAAACGCTACTAAAACCGCAGGTAAGATTGCTGGTTTAAATGTTGAAAGGATCATTAACGAACCAACTGCTGCTGCTTTAGCTTATGGGATTGATAAAGCATCAAGAGAGATGAAAGTCTTGGTTTATGACTTGGGTGGTGGAACTTTTGATGTATCTTTACTTGACATTGCAGAAGGTACTTTTGAAGTACTTGCAACTGCTGGGGACAACCGTTTGGGAGGTGATGATTGGGATAACAAGATCATTGAATATATCTCAGCCTACATTGCCAAAGAACACCAGGGTTTAAACTTATCAAAAGATAAGATGGCAATGCAACGGCTTAAAGAAGCAGCTGAACGTGCTAAGATTGAACTTTCCGCTCAACTTGAAACGATTATTTCTTTACCATTTTTAACTGTTACCCAAAAAGGTCCTGTTAACGTTGAGTTAAAACTAACCCGTGCTAAGTTTGAGGAGTTAACAAAACCACTACTTGAAAGAACAAGAAACCCTATTTCAGATGTTATCAAGGAAGCTAAGATTAAACCTGAAGAGATTAATGAAATTCTTTTAGTTGGTGGTTCTACAAGGATGCCTGCAGTTCAAAAGCTAGTTGAATCAATGGTACCAGGTAAAAAACCAAACCGTTCTATTAATCCTGATGAAGTTGTTGCTATTGGCGCTGCTATTCAAGGTGGGGTTTTACGTGGTGATGTTAAGGATGTTTTACTTTTAGATGTAACTCCATTAACCCTTTCTATTGAAACTTTAGGTGGTGTGGCTACTCCTTTAATTAAGAGAAATACAACTATCCCAGTAAGTAAAAGTCAAATCTTTTCAACTGCTCAAGATAACCAAGAATCAGTTGATGTGGTTGTATGTCAAGGGGAAAGACCAATGTCTAGAGATAATAAGTCATTAGGAAGATTTAACTTAGGTGGTATTCAACCAGCACCTAAAGGTAAACCCCAAATTGAGATTACCTTTAGTTTGGATGCCAATGGGATCTTAAATGTTAAAGCTAAGGATTTAACCACGCAAAAGGAAAACAGTATTACCATTAGTGACAACGGTAATCTTTCTGAGGAGGAGATCCAAAAGATGATCCGTGATGCTGAAGCTAACAAGGAACGGGATAACATCATCCGTGAACGTATTGAATTACGTAATGAAGGGGAAGGTATTGTTAATACCATCAAAGAGATATTAGCAAGTCCTGATGCTAAGAATCTCCCTAAAGAAGAAAAAGAGAAGTTAGAAAAGCTAACAGGTAACATTGATGCTGCTATTAAAGCTAATGACTATGCCAAACTCAAAGTGGAAATTGAAAACTTTAAGAAGTGA", "aa": "MSADNGLIIGIDLGTTNSCVSVMEGGRPVVLENPEGKRTTPSIVSYKNNEIIVGDAAKRQMVTNPNTIVSIKRLMGTSNKVKVQNADGTTKELSPEQVSAQILSYLKDFAEKKIGKKISRAVITVPAYFNDAERNATKTAGKIAGLNVERIINEPTAAALAYGIDKASREMKVLVYDLGGGTFDVSLLDIAEGTFEVLATAGDNRLGGDDWDNKIIEYISAYIAKEHQGLNLSKDKMAMQRLKEAAERAKIELSAQLETIISLPFLTVTQKGPVNVELKLTRAKFEELTKPLLERTRNPISDVIKEAKIKPEEINEILLVGGSTRMPAVQKLVESMVPGKKPNRSINPDEVVAIGAAIQGGVLRGDVKDVLLLDVTPLTLSIETLGGVATPLIKRNTTIPVSKSQIFSTAQDNQESVDVVVCQGERPMSRDNKSLGRFNLGGIQPAPKGKPQIEITFSLDANGILNVKAKDLTTQKENSITISDNGNLSEEEIQKMIRDAEANKERDNIIRERIELRNEGEGIVNTIKEILASPDAKNLPKEEKEKLEKLTGNIDAAIKANDYAKLKVEIENFKK", "aa_hexdigest": "5c65c74fa307ad5c2e829b547fa48601", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47547", "query_cov": 1.0, "subject_cov": 0.9663865546218487, "identity": 0.998, "score": 1080.0, "evalue": 0.0, "valid": true, "gene": "dnaK", "product": "molecular chaperone DnaK", "uniref50_id": "UniRef50_P9WMJ9", "cog_id": "COG0443", "cog_category": "O", "go_ids": [ "GO:0005524", "GO:0006457", "GO:0051082", "GO:0140662" ] }, "pscc": { "uniref50_id": "UniRef50_P9WMJ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P9WMJ9" ], "product": "Chaperone protein DnaK" }, "genes": [ "dnaK" ], "id": "JPCNHPFPBM_698", "locus": "GCA000292505_00683" }, { "type": "cds", "sequence": "contig_1", "start": 376645, "stop": 376908, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGTAGCAATTGTTTCCTACTTTAGTTTGTTTGGTGTTTTTATGATCACAAAAACTGTCAACACAACTAATAACGTTATTGATCAATACAGCTTAATTGATCTTTTTAACATTACTAATATTGCTGTTACCGCTTCTAACTTTATTCCAGTTGCTAGTGGGGAAGGTGCAACACAATTTGTGATGACTAGTTTTCTCAATGCATTCAAACCAACTGATCAATTCCTCCATGATCAGATTAAAGATGGTGTTTTTTTATGA", "aa": "MVVAIVSYFSLFGVFMITKTVNTTNNVIDQYSLIDLFNITNIAVTASNFIPVASGEGATQFVMTSFLNAFKPTDQFLHDQIKDGVFL", "aa_hexdigest": "0e818fcc072ad2cf1ab783cceb27ea3d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9603.948199999993, "isoelectric_point": 4.451254844665528 }, "id": "JPCNHPFPBM_699", "locus": "GCA000292505_00684" }, { "type": "cds", "sequence": "contig_1", "start": 377404, "stop": 377676, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAGTTAACCACCAATACCTTTTTTAACACTAAAAACATCGTTGCTTTTAGCTTTTTTTTGGTTTTTCTAATCGTTATTAGTGTTATTGTTACTATCTTTTTTTTAGGTGTTAGTGTTGATGATGTGAAAACTATTATCACTGCAATTAACTATCAGAATTGGGGTTGGATATTTGTTGTTATCTTAGGGTTTTTAGTTTCAGTTTTGTGGAATGTAATCATTAATTGGTGGGTATCAAGGAGATTTTGTTTTTATGCTAGTTGGTGA", "aa": "MAKLTTNTFFNTKNIVAFSFFLVFLIVISVIVTIFFLGVSVDDVKTIITAINYQNWGWIFVVILGFLVSVLWNVIINWWVSRRFCFYASW", "aa_hexdigest": "da1c4f127275045829134d49d3f9934c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10539.441099999998, "isoelectric_point": 9.514002418518068 }, "id": "JPCNHPFPBM_700", "locus": "GCA000292505_00685" }, { "type": "cds", "sequence": "contig_1", "start": 377676, "stop": 378728, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGGTAATCCTAATAATGGCAATATTGATGAATTGTTTAAAAAAGCTTTTTTAGAGAGTGTTGATGAATCAGGTTTTAATGTTAACTTTAAAGCAGTTATTGACCATTATCGCTTCATTTTTACTTTGCAATGGTTAATTGAAAACAACCTAAAAAACTTTAAAGATATTTTACAAGCTAACTTAAAGTATGGTGAAATTGCCTATATTGCTTATGATAAGAATATTATCAGTAACAACACCAATAATCCTCAGGGAATATTTGGCTCTGTTTTTAACTATGAAAATGATGAACATGCTACAACAGCTAATGCCAACCAAACTATTGATCCTAATAATTTCTTTTTTAAAACCAAAACAAGTCCTAACACTACACCTGCTACTACGATGTTAAGCACCAGACAAGCAGTATCAACAAGTAATAATGGTACTTATGGTTTTACTGGTTTGAATACAACTAACAGTTCCATGTTAGAAAACAATACTAACCAGGCACTTTTAAACCATATCGCTGCCAATTCCTTAAAACAATATGGTTCAAAGGATGATCTTAAAAAATTTATTAGTGAAACAAAAGACCAATTAGTTTTAGATAACATTGCCCGACAGCTAAGTAGACTAACTCCAAGTAGTAGTAGTAGTAATGGCAAAACACTTTCAGCTTATTTTCAGGTCGATGCAATTAACAATTCTGCTTTAGATTTCAAAGCTAAACAAGCGTTGTTATTAGCTGTTTTAGACCAATATTCCAGTTATTTTAATTCATCAAATAGTCCAAGTATTTCAAAAAGAAGTACCCAAACCAACCAAAAATTTAGTGAATTTAACTTTGGTGGTGATAGTTACAACTATCTTCAGTTCACTAAAAGTGACATTGATTCACTTAGTTTAACAAGTTCTACCAATATTGAAAGTGATATAGTAGCAGCTTTAGTTTTATTTCAAGCTAGTGATACAGGCACCCAACAACTTGCTTTAAGTGCAATTGAAAAACCCCAATTTAGGATTGGTGATAAAAGAATTCAATCAGGCTTAAACTTGCTTAAGTAA", "aa": "MYGNPNNGNIDELFKKAFLESVDESGFNVNFKAVIDHYRFIFTLQWLIENNLKNFKDILQANLKYGEIAYIAYDKNIISNNTNNPQGIFGSVFNYENDEHATTANANQTIDPNNFFFKTKTSPNTTPATTMLSTRQAVSTSNNGTYGFTGLNTTNSSMLENNTNQALLNHIAANSLKQYGSKDDLKKFISETKDQLVLDNIARQLSRLTPSSSSSNGKTLSAYFQVDAINNSALDFKAKQALLLAVLDQYSSYFNSSNSPSISKRSTQTNQKFSEFNFGGDSYNYLQFTKSDIDSLSLTSSTNIESDIVAALVLFQASDTGTQQLALSAIEKPQFRIGDKRIQSGLNLLK", "aa_hexdigest": "45e598f59d95c5711f9933ff31388b56", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 38871.57880000007, "isoelectric_point": 5.976699638366699 }, "id": "JPCNHPFPBM_701", "locus": "GCA000292505_00686" }, { "type": "cds", "sequence": "contig_1", "start": 378750, "stop": 379457, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAAGCATTCAATGATTACAAAGAACTAATGCAAAAATTTGCACCAGTTGCATTAGCAACAAATGTAATCAGAGATTTGTTTTTACAAATGGATGCATTAGATAACAAACTAACAAGTAGAACAACTGAACTTAATACCAATGTTAACCAAATTAATCCAACCCCTTGGTTAAATGGCTTATCTTCCCATTTGCCATATGTTCGCAAGTCTGGTCATTATGAAAAACTCAATAACTATTTCTTGTTTTTAATTACTAAAACACTTTGGAAAAAAGTAGGTAGTGAAAAAATAAATATTAGTGAAAACAACAATAAGTTAAAAACTACAAAAGCTGATGTTGATAAGATAAGAGATGAAATTCTGAGCGACATAAATAACAAAGTAACAGAATTTGTTAACCAATTGAAAGTTACAGAAAAATCAAAACCTAACTTTAGCAACATTATTCTAGTTGATATTAACAATGATCAATCACTTACTAACAGTGCCAATTGGAGTTTAAATGCGTTGTTAGATGTAACAACTGTAAACCCTCTTTCCTTTGCATTGTTAAAAAATGCTTTTACTAGCAATCAACAGTTTGAAAAAGCTAAAAAGCTATTTGAAGAGATTAAATCTAAAAATGGATCTTCATCAACTTCATCAAGTAGTGATGCAGATAGTTTAGCTAAAGTAATTAGCAATTACTATTACATGACATGA", "aa": "MQKAFNDYKELMQKFAPVALATNVIRDLFLQMDALDNKLTSRTTELNTNVNQINPTPWLNGLSSHLPYVRKSGHYEKLNNYFLFLITKTLWKKVGSEKINISENNNKLKTTKADVDKIRDEILSDINNKVTEFVNQLKVTEKSKPNFSNIILVDINNDQSLTNSANWSLNALLDVTTVNPLSFALLKNAFTSNQQFEKAKKLFEEIKSKNGSSSTSSSSDADSLAKVISNYYYMT", "aa_hexdigest": "675dc3db95f98a847ed45261eceb5c4f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26675.922800000026, "isoelectric_point": 9.155235862731931 }, "id": "JPCNHPFPBM_702", "locus": "GCA000292505_00687" }, { "type": "cds", "sequence": "contig_1", "start": 379623, "stop": 380054, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCTGTGAACACAATACAACAGACAGTTCACAAAACAATTTAAAGAGTCTTATTAAAGATGCTTTTATAAGTTCTAATGATAGTTCCAATTTAGGAAAACTAGCAAAGCAAATCCACCAAACAACTACAAGTGATATGATGGTTTCAACTAAAGCTAGTTCATCAACAACAAGCAGTTATCTTGTTTGGGATGCAGCTATTCCTAATAACAAAACAAATAATGGTGCTAGCACTGTTAGTGCAAACTGTGCAAATGCTACTGTTCAAAATACAAGCCATAACTCTAATAACCAATTGAAGCTAAGACTAGTTAGAAACGGAGAGGGAGTTGCTGTAATTGGAATTGATGGAGGTAGTTATTATCTGACAAAAAATAGTAGTAGCAAAACTGAAAGAGACATTGAAAAGCAAAAGCAGTTTTTAATGTGA", "aa": "MFCEHNTTDSSQNNLKSLIKDAFISSNDSSNLGKLAKQIHQTTTSDMMVSTKASSSTTSSYLVWDAAIPNNKTNNGASTVSANCANATVQNTSHNSNNQLKLRLVRNGEGVAVIGIDGGSYYLTKNSSSKTERDIEKQKQFLM", "aa_hexdigest": "13139f6c43c45cbaa3b7401ad532b5c7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15470.92739999998, "isoelectric_point": 8.928565025329593 }, "id": "JPCNHPFPBM_703", "locus": "GCA000292505_00688" }, { "type": "cds", "sequence": "contig_1", "start": 380376, "stop": 380600, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGCAAAGTTACAAGCACAAATCTTTAATCTCTGGGTTGAATATACCGATCCTACTTTAATCAGTCAAGCTACTTTTAAATATGCAGCACCACAACAAGGTTTGGGTCAAGTTTACAACACTGAAAAACTAAAAGATAAATTAACCCCTTCTTATGCGTTTCCTTTCTTTCCTAAAGATGGGGAAATTCCTCAGAACCAGAATGTTGGCAACAAACGCTGA", "aa": "MFAKLQAQIFNLWVEYTDPTLISQATFKYAAPQQGLGQVYNTEKLKDKLTPSYAFPFFPKDGEIPQNQNVGNKR", "aa_hexdigest": "e9a883727d7d1cffa9a817c3bf33fddb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8485.5487, "isoelectric_point": 9.004250907897951 }, "id": "JPCNHPFPBM_704", "locus": "GCA000292505_00689" }, { "type": "cds", "sequence": "contig_1", "start": 380790, "stop": 381209, "strand": "-", "frame": 2, "gene": null, "product": "putative lipoprotein MPN_442", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75336" ], "nt": "ATGAAAAAACTTTTAAAAAAATCAAAGTTCTGGTGGTTTTTACTTTGTGGTTTGTCAGTAAGCACAATACTTGTAGCTTGTTCAACTCCAACAAACAGTGCTTTGCAAACAGTTTTTTCACCAACCTCAACTAATTTTTTTCATGGTCAAAAGGGTAGTTTAAAAGAAGGACTTATTACTGCTTTAAAAACTCCTTCAGCAAACAAACATTTTGTTATAGGACCACTTTTAAAAGCACTTGAGGCTTGGTATGAAAACAATGAAGACAAAGCCATAAACAAGTTTTTAAAAGACACTAAAAAGAATGTTGATGATCAATATAAAAATACTGTTAATGGGTTAATATCACCACCCAGAAACAGAGCAGTATTTATTCAACAAGATCTATTAGATAAAAGTGGTGGCAGTGAGGCTAGTTGA", "aa": "MKKLLKKSKFWWFLLCGLSVSTILVACSTPTNSALQTVFSPTSTNFFHGQKGSLKEGLITALKTPSANKHFVIGPLLKALEAWYENNEDKAINKFLKDTKKNVDDQYKNTVNGLISPPRNRAVFIQQDLLDKSGGSEAS", "aa_hexdigest": "10e6f857f9c6bac6b02964ccfe5aeb4d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P75336", "query_cov": 0.9784172661870504, "subject_cov": 0.9006622516556292, "identity": 0.603, "score": 159.0, "evalue": 9.37e-48, "valid": false, "product": "Uncharacterized lipoprotein MPN_442", "uniref50_id": "UniRef50_P75336", "go_ids": [ "GO:0098552" ] }, "pscc": { "uniref50_id": "UniRef50_P75336", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75336" ], "product": "Uncharacterized lipoprotein MPN_442" }, "genes": [], "id": "JPCNHPFPBM_705", "locus": "GCA000292505_00690" }, { "type": "cds", "sequence": "contig_1", "start": 381206, "stop": 381382, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGTTTTTGACTGAAAATAACCAAAAAACTACCAAAAAACCGCTTTCAACTTTACAAATTAAGAAAATTAAAGCTATCAAAGCTAAATATAAAAAGGTTAAACCTAACTACAAAAAATACCAAAAACAACAGATTCAAAACCTTTTAATTAATAAGAAAAAACCAAGATCATGA", "aa": "MKFLTENNQKTTKKPLSTLQIKKIKAIKAKYKKVKPNYKKYQKQQIQNLLINKKKPRS", "aa_hexdigest": "db354a7ab134bfb140c51409a315f68c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6960.372600000004, "isoelectric_point": 10.623052024841307 }, "id": "JPCNHPFPBM_706", "locus": "GCA000292505_00691" }, { "type": "cds", "sequence": "contig_1", "start": 381554, "stop": 381970, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00271.37" ], "nt": "TTGTTTGCCTTTTTTAGTGCTACTTTATTCAATCAACAACTCCAAATAATTAAAAAACAAGTAATTAATACTAAAGTTATTAATCTCCATCCAAAGCAATGGATTCACCCCTTAGTTAAGCATTTTGTTGTTCATCTCAATACTGAAAACCGTTTTTCTGGTTTATTAGCCTTATTAAAACACCACCAAAATCAGCAAATTATTGTTTTCTGTTCTAATCAAAAATCTTTAAAACAATTAACACAATTGCTTTCAAATAACAATATTAGTTTTGGTTCAATTTATGGTAGTTTAACCTATCAAGAACGTAAAAATAATTTCACAAAAGCAACTAATAATAAATTGAAATTACTAGTTGTTTCTGATCTATTTTCACGTGGGATAGATTTAAATTATTTCAGTGTGGTGATTTCTTGA", "aa": "MFAFFSATLFNQQLQIIKKQVINTKVINLHPKQWIHPLVKHFVVHLNTENRFSGLLALLKHHQNQQIIVFCSNQKSLKQLTQLLSNNNISFGSIYGSLTYQERKNNFTKATNNKLKLLVVSDLFSRGIDLNYFSVVIS", "aa_hexdigest": "7e2a69c49f7eff7cbea2f7755ce40229", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00271.37", "name": "Helicase_C", "length": 85, "aa_cov": 0.6159420289855072, "hmm_cov": 1.0, "evalue": 1.2584054206875637e-17, "score": 59.229312896728516, "start": 53, "stop": 137 } ], "seq_stats": { "molecular_weight": 15959.388299999986, "isoelectric_point": 10.178349113464353 }, "id": "JPCNHPFPBM_707", "locus": "GCA000292505_00692" }, { "type": "cds", "sequence": "contig_1", "start": 382448, "stop": 383257, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAAAGTGCACAGCAAACCAATAACCAGCAAAGAAGGTTTGGTTTCCATGGTATTGTTACCAACACTTCATCCAACAACTTACCAGATGCAGTTAGAAATCGCCTCTTTACAAGTTTTGTTTCCCAATCTGAAAAATCAAGTTCTAATGGTGGTCAAGCACAATTACAATCTACTCAATCAAGTGGTAGTAATGAAACAATTTACAAAGGTGCTTTATTTAGTTTTGGTTCTCTAACAAAACTTATAGAAACAATTGATAACATCCCAACCCAAGCTGAGTTTGATGCACTTTATAACCATTTAACAAGTAACCTTAATATTAACGTTACTGGTGTTGATAGATCAAAATCCTTACAAGAACAAAAAACTAATTTGAAGAATTTTGCTAATTCAAACTTTAACAATACTCAAACTGTTCAATTAAAACAAGCTCAAAGTAAAACTAATAATAGTAACTTTAATGATGTGTTTAGTAGGTTTGAAGGGTATATAGGTACAAATAAAACCAGTAATTATAGCAGTTATAACTTTTTACAAGATAACCAGATCTATCATGCAGTTTATGCAAAACAGATTAACCTTGAAGATGTAAGTATGCTTGGTAGTGATAGTTTGAATAGTACAGATTCAAATAATTCAAAAAGACTTGATCTTTCACTTGAAGAATTTTTAAGTACAGTTGCGCTTGAAGCGCTTAATCCTAATAATCAAACCCAAGCTATTAATGCATTGATAGCAAATGCCAAAAATGGCTTAGTTAGAGTTGGTGATAACAGATTGTTTTCAGCAATCAGTTCACAGTGA", "aa": "MQKSAQQTNNQQRRFGFHGIVTNTSSNNLPDAVRNRLFTSFVSQSEKSSSNGGQAQLQSTQSSGSNETIYKGALFSFGSLTKLIETIDNIPTQAEFDALYNHLTSNLNINVTGVDRSKSLQEQKTNLKNFANSNFNNTQTVQLKQAQSKTNNSNFNDVFSRFEGYIGTNKTSNYSSYNFLQDNQIYHAVYAKQINLEDVSMLGSDSLNSTDSNNSKRLDLSLEEFLSTVALEALNPNNQTQAINALIANAKNGLVRVGDNRLFSAISSQ", "aa_hexdigest": "5210017a7e8bff392c49153ed5b0fd3c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29728.128400000034, "isoelectric_point": 7.891591072082519 }, "id": "JPCNHPFPBM_708", "locus": "GCA000292505_00693" }, { "type": "cds", "sequence": "contig_1", "start": 383357, "stop": 383488, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGTAAGTTCTTCTAAATTTGTATTAGTAAAAATGGCTAACAACGTTGCTTCTCAAACTAATCAAGACTTTACCAAGCAAGAACAACAAAATCCTAACTATGTATTTGGTTCGAGTGCTTATAATTGA", "aa": "MSVSSSKFVLVKMANNVASQTNQDFTKQEQQNPNYVFGSSAYN", "aa_hexdigest": "424b238f148e7ab6fbc914beafb908e9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4790.216700000001, "isoelectric_point": 8.1392786026001 }, "id": "JPCNHPFPBM_709", "locus": "GCA000292505_00694" }, { "type": "cds", "sequence": "contig_1", "start": 383849, "stop": 384217, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAAAGTTAGTTCAAATACAAGTAATAGTAATAGTAATAGTAAATAGTAGTAGTAGTAGTGATCCTTTTTCAAAAAAGATAATTGACAAGTTAAAAGAGAATAAAAAGAAAGTTGAAGCAGCAGTTAAAAAGCATGTTGATGAACTAAAAGTTTCGGTAATTCCCTCACCGCAATATTCACAAATTATCCTTGTTGATACCAAACTTTCATCTGATCCTAGAAACACTTCTCTAGCACTTAATTTAGCTTTAAATGCTGTTTTATCAAGTGATGAGTTACAAAATACCATTAGACGAGATTATTTTGTCAATGATGATCAGTTTAAACAAGCGATTGATTTAGATAAATTAACTTTTAAAAACTGA", "aa": "MIKLVQIQVIVIVIVNSSSSSDPFSKKIIDKLKENKKKVEAAVKKHVDELKVSVIPSPQYSQIILVDTKLSSDPRNTSLALNLALNAVLSSDELQNTIRRDYFVNDDQFKQAIDLDKLTFKN", "aa_hexdigest": "b3a4e7662e78d64bd96dd1f8b93f2dae", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13740.745399999978, "isoelectric_point": 9.126031684875489 }, "id": "JPCNHPFPBM_710", "locus": "GCA000292505_00695" }, { "type": "cds", "sequence": "contig_1", "start": 384168, "stop": 385169, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAAAGCTAGTGATATGTTTGATACCTTTGATCCTAGCTTTTCAGCAGCTTTTATCCAGGGCTATTTAGCTTTACAAAAAAAATCCAAAGGTGCAGAACAAACTGAATACACTAAATTAGAAAAAGATAAATCAATCATTGAAAATTTCTTTGTTGAAAATAATTCAGCTAAGGCTGCAATGAAAAGTGCAAGTAGTAGTTCACAAACAACTACTGTTCATAAAACAGATCTAGCTAAGATCTTTAAAGAAAATGAAACTACTAAATCAACAGATGTTTTTAAAGGTGAATATCAAAAAAAGTTCAGTAATGCTACTAGTACAAGTAACAGTGATAGTAGTAATAATTCTGCAATCGTTGATCTAAAAGAACTTAAAAAAGATAACAATAGTCAACCTGATTTAATCTTAGCACGTGGTAAAGATGGTATCCATCTAATGGGTGTTGATGGTGGTGGTTATTATCTCTCTGAATCAGGACGTGATGTAAATAAACAAAAACAATTCTTATTATTCCGTGCACTACAAACCAAATATGGCTTAATTGATACAAACACTACTTATGACTTTAAGTTGTTTGATGAAGTGAAAAAACACTTTGATAAGAACAGAGTTTTATTTTTATTTAATGCCCTTTTTAAATTAGTAGACAGCAAAGAAAGTAACTTTTTAAGTTTCCCTCAGTTTAAAAAGTTTAGTGATAGCATTATCACTGTTAAAAACGAGTTGAAGGATTTAGTTGAAAGTCAATATCAACAAATTGTTTTTAATGAAGTTGCTACTGCAGAAAACAAAGTTGCTTTAAAACTAGCAGAACGTAACCAACCTTTCATTGATAATGAGCGAAATAAACAAATATGGATGAACGGTTTAGCTGCTGTTTTACCTTATGAACAAGATAGTAAAACAGGACACTACAATGAACTTGGTATTTACTATAAAGACATCATTGATAAAGTTAGTTCAAATACAAGTAATAGTAATAGTAATAGTAAATAG", "aa": "MLKASDMFDTFDPSFSAAFIQGYLALQKKSKGAEQTEYTKLEKDKSIIENFFVENNSAKAAMKSASSSSQTTTVHKTDLAKIFKENETTKSTDVFKGEYQKKFSNATSTSNSDSSNNSAIVDLKELKKDNNSQPDLILARGKDGIHLMGVDGGGYYLSESGRDVNKQKQFLLFRALQTKYGLIDTNTTYDFKLFDEVKKHFDKNRVLFLFNALFKLVDSKESNFLSFPQFKKFSDSIITVKNELKDLVESQYQQIVFNEVATAENKVALKLAERNQPFIDNERNKQIWMNGLAAVLPYEQDSKTGHYNELGIYYKDIIDKVSSNTSNSNSNSK", "aa_hexdigest": "a446a1a23703cac708998295e4eb60a0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 37702.803400000004, "isoelectric_point": 8.320950508117676 }, "id": "JPCNHPFPBM_711", "locus": "GCA000292505_00696" }, { "type": "cds", "sequence": "contig_1", "start": 385692, "stop": 386054, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGAATTGTTGCTGTTGCCTGTGCACAACCAAACTCCAGAACCATTGAAAATCTCTTTAGACCCAGTAGTGCTTTTACTGATAAAAATGATGGTAGTATTAATGCCACACTTTATAAAGCACTTGAAAATAGAGAAGGACTAACGCAATATTTAACGATGCGCCTAGCGCCAGTATTGCGCAATTTTTATGAAGAAAATGTTGATGATGACATTAAGAGAAATTTAAGAACTTTTAATACTGATACAGACAATAGTTTTGTTAATCAAGAACAAAACTTAAGAAATCAGTATCGGGGTGATTATTTAGTTAGGTTACAAACTGATATTTTAGATAACACTGGTGGAAATCAAGCCAACTGA", "aa": "MGIVAVACAQPNSRTIENLFRPSSAFTDKNDGSINATLYKALENREGLTQYLTMRLAPVLRNFYEENVDDDIKRNLRTFNTDTDNSFVNQEQNLRNQYRGDYLVRLQTDILDNTGGNQAN", "aa_hexdigest": "f6b597b7100c4a3218f8b2035cabfd0a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13696.93039999998, "isoelectric_point": 4.871010780334472 }, "id": "JPCNHPFPBM_712", "locus": "GCA000292505_00697" }, { "type": "cds", "sequence": "contig_1", "start": 386168, "stop": 386383, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGACTTGAAATAGAAAACGGGCTTGAATTTGTCAATGATCCTGTGGTAAATGAACTTGGCAAGATCTGTTTTTTTCATCCTTTTACAGGTAATTTAACAAACAAACTTAGTTTCAGAAGTCATTTCAATAGATACAGTTTTTATGCCATTAACTACCCAGGGCATGGTAATAGTGTTATTAACAATCCAAAGCAACTTGAATTTAGTTACTGA", "aa": "MRLEIENGLEFVNDPVVNELGKICFFHPFTGNLTNKLSFRSHFNRYSFYAINYPGHGNSVINNPKQLEFSY", "aa_hexdigest": "0559d41656c46e83637a9a9b1e1bb444", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8287.231399999997, "isoelectric_point": 6.815188407897949 }, "id": "JPCNHPFPBM_713", "locus": "GCA000292505_00698" }, { "type": "cds", "sequence": "contig_1", "start": 386384, "stop": 386797, "strand": "+", "frame": 2, "gene": "menH", "product": "Putative esterase/lipase 1", "db_xrefs": [ "COG:COG0596", "COG:HR", "EC:3.1.-.-", "GO:0016020", "GO:0016298", "SO:0001217", "UniRef:UniRef50_P75333", "UniRef:UniRef90_Q49412" ], "nt": "TTGGAAATTACAAAACAGTTTTTTGATAAACATAACTTAAAGGATGTAATCTTATTTGGTCATTCTATTGGCGGTGGTTTAGCAGTTGCTTTAACTAACTATTTAAGCAGTGATCAATATAAAGCAGTCTTGTTAGAAGCACCATTAAATCCTGCTATTGTTGAAACTCCTTTAAATATTGTTCAAAATCTCATTCCTGATCCTGATAGTGATTTTGCTGTTATTCAAAAGTGTCTTGTTTACAATATTGAGAAAAAACTTGGGGCAAACTTTAAAGAATATTGTGAAAGAGAAAAGCAAAAATCAATTCACCAAAACCAACGCCTTAAAGTGATGTTAGAACCTTCTACACTCAAACAAAACATAGTTTTAATCAATGCAGCTTTTTTAAAGTTAAATTGTCCTGCTTTGTGA", "aa": "MEITKQFFDKHNLKDVILFGHSIGGGLAVALTNYLSSDQYKAVLLEAPLNPAIVETPLNIVQNLIPDPDSDFAVIQKCLVYNIEKKLGANFKEYCEREKQKSIHQNQRLKVMLEPSTLKQNIVLINAAFLKLNCPAL", "aa_hexdigest": "117a70ad617aa154477b429457924bb0", "start_type": "TTG", "rbs_motif": null, "genes": [ "menH" ], "truncated": "both", "pseudogene": { "start": 386168, "stop": 386974, "observations": { "mutation": [ 386381, 386795 ], "stop": [ 386381, 386795 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49412", "query_cov": 1.0, "subject_cov": 0.5111940298507462, "identity": 0.993, "score": 266.0, "evalue": 2.03e-88, "gene_start": 1, "gene_end": 137, "reference_start": 73, "reference_end": 209, "reference_sequence": "MRLEIENGLEFVNDPVVNELGKICFFHPFTGNLTNKLSFRSHFNRYSFYAINYPGHGNSVINNPKQLEFSYWLEITKQFFDKHNLKDVILFGHSIGGGLAVALTNYLSSDQYKAVLLEAPLNPAIVETPLNIVQNLIPDPDSDFAVIQKCLVYNIEKKLGANFKEYCEREKQKSIHQNQRLKVMLEPSTLKQNIVLINAAFLKLNCPALWIHGKQDGIIKYLPSKAYYESLNNKQIQFKAIEAAAHTPYFEQPQKFLSLVNDFFQLIS" }, "paralog": false, "identity": 0.9925373134328358, "score": 536.0, "evalue": 2.77e-197, "description": "Internal stop codon at 386381, 386795. Nonsense mutation around 386381, 386795.", "psc": { "uniref90_id": "UniRef90_Q49412", "gene": "MenH", "product": "Putative esterase/lipase 1", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75333", "cog_id": "COG0596", "cog_category": "HR", "go_ids": [ "GO:0016020", "GO:0016298" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49412", "EC:3.1.-.-", "UniRef:UniRef50_P75333", "COG:COG0596", "COG:HR", "GO:0016020", "GO:0016298" ] }, "pscc": { "uniref50_id": "UniRef50_P75333", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75333" ], "product": "Putative esterase/lipase 1" } }, "id": "JPCNHPFPBM_714", "locus": "GCA000292505_00699" }, { "type": "cds", "sequence": "contig_1", "start": 387002, "stop": 387598, "strand": "-", "frame": 3, "gene": "rpsD", "product": "30S ribosomal protein S4", "db_xrefs": [ "COG:COG0522", "COG:J", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0042274", "KEGG:K02986", "SO:0001217", "UniRef:UniRef50_P46775", "UniRef:UniRef90_P47553" ], "nt": "ATGAAATATACTGGTAGTATTTTCAAACGATCAAGACGTTTAGGTTTTTCTTTACTTGAAAACAACAAAGAGTTTTCCAAAGGAAAAAAACGTAAATCTATTCCCGGTCAACATGGAAATAGGTTTCGTTCCTCAACTTTATCAGGTTATGCCCAACAACTCCAAGAAAAACAAAGGATGCAATACATGTATGGTATTACTGATAAACAATTTCGTAGGTTATTTCGCTTTGTTTTAAAACAAAAAGGTAACTTAACAGTTAATTTATTTAGAGTTTTAGAATCACGTTTGGATAACATAGTTTACAGAATGGGTTTTGCACCAACAAGAAAGAGTGCAAGACAAATGGTAAACCACGGTCATGTGATTTTAAATGATCAAACTGTTGACACCCCTTCAATCATCATTAATCCCGGGGATAAAGTCCGTTTAAAAGCAAGAATAACTAAATCCCCATTAGTTAAAAATTTTATTGAAAACAGTGTTATCTCATCATTTGTGGAAACCAACAAAAAGGCATTTGAAGGTACTTATATAAGATTCCCAGAGCGTAGCGAACTACCTGCTGGAATAAATGAATCTTACGTTGTTGAGTGA", "aa": "MKYTGSIFKRSRRLGFSLLENNKEFSKGKKRKSIPGQHGNRFRSSTLSGYAQQLQEKQRMQYMYGITDKQFRRLFRFVLKQKGNLTVNLFRVLESRLDNIVYRMGFAPTRKSARQMVNHGHVILNDQTVDTPSIIINPGDKVRLKARITKSPLVKNFIENSVISSFVETNKKAFEGTYIRFPERSELPAGINESYVVE", "aa_hexdigest": "e0a00388f78d267ac1d8e235e27a2c68", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47553", "query_cov": 1.0, "subject_cov": 0.9658536585365853, "identity": 1.0, "score": 384.0, "evalue": 6.92e-135, "valid": true, "gene": "rpsD", "product": "30S ribosomal protein S4", "uniref50_id": "UniRef50_P46775", "cog_id": "COG0522", "cog_category": "J", "kegg_orthology_id": "K02986", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "GO:0042274" ] }, "pscc": { "uniref50_id": "UniRef50_P46775", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P46775" ], "product": "Small ribosomal subunit protein uS4" }, "genes": [ "rpsD" ], "id": "JPCNHPFPBM_715", "locus": "GCA000292505_00700" }, { "type": "cds", "sequence": "contig_1", "start": 387621, "stop": 389756, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCATTATGAAAGTAAATCTGAACCAATTCATGACTATAAATTTGGGAGTGATTTATCTCAATCAAACAGCAATAATTCGTTAGAGTCTGAACCTGTTAAGTTTAATAGTGAAACTGCACCTGATGCACACTTTGAAAGTCAATCTGAACCAGTTGATCAAGTTCAATACGACATTTATCAAAATGAAGAGCTAAAACCAACTCTTGACCAGCCTTCTTCAGATGATTATTTTGCAAAACAACCAACTGATGAAAACTATGGTTTTGATAACGACCTACCACCAGAAGTAAAACAACCTGAATCAGTTGTTGACCAACCTTCTTCAGATGATCATTTTGCAAAACAACCTGAATCAACAACTGATAGCTACAGCTTTGACAGTGATTTACCTCAACCAACCCTTGACCAACCTTCTTTAGATGATCATGTTCAGTACAACTTTGATCACCATGAAGAGCTCAAACCAGTTGCTGAAGAACAAAATAATTATCAAGTTGGATTTGATCAAGTTCAAGCTAATCTTGATAATAATGAGGAAATACAACCAACTGCTGAAAAAAAAGTAACTACTGATTTTGAAAGTAAACAAGCCCAAGTAGTTGATAGTTACCAATTACCAATTGATACAGATCAACAAGACCAAACAACTTTTTCTTCTTCATTTGAAACTCAACCAACAGTTGAGCAGTTTGATCAAGTAAATAGCGAATTTAACGATCAATTCAAATCTGAAATAACTAAGGAGCCAGTTTTAGAAAGTAGTTTCAATAAACAAGATGTAGTTGAAACTAGTAACTACACTAATAATTTACAGAAATTTGATATTCAGTCTGATAATAAGATAACTATCACTACCAAAAAAAGTTCACCCCAAATCCCTACAACTTTACCTATTAGTTTTGTATCCAACCGTATTGAATATAAACCAGTTGAAACTTTAGCTTTAGATAATAAAGAAAGTCAGCAAGAACAAATTACCATTAATTCCATAACTGAAGATTCCAAAACACTTGCAAAAACATTATCTGTCCAATTGCAACAAATTAATAGCTTAAATAATCAATCAATTGTTACTAGTGAATCAGTAAGACTCGATAAAAAAGATGATCAACTAACTATTAATACTGTTAATAGCGAAGACCAACAACCTAAAATTGAAGTTTTTGTCAAAGCAAAAGAACCTGTTGAAGAACACTCAATTACTCAAAATAAGCAATCGGTTGAAGATAAAAGTGAACTTGATAATTTCAATAAAAAATCAGATCTTTACAAGATTATTAGTGAATTGAAACGTGGTGAATTAAACCCTACAATTAACTTTGATGCAATCTTTCAAATGAATGATTATCAGATGTCTGTTAAACAATCATTTATCCATCTCAATGATTTTGTTACCAACTATAAGAACCAAATTAGTGAACGTTACTTAATAATTAAAAAGGAATTACAAAGTGAACTTTCCCGTTTAATTGATCAAAATGAGAACCTTAACGTTCAATTTAATAACGCTAAGAATCTAACAACACTTCAAAAAGAAGAAATGATCAGATCATTAGCTAGTGATTTTGCTATTGCTTACAAACCAAGTAATAGTTATGAACAACTGCAAAAATCTGGAGAAATTATGCGTCATGTTCAACGTGCTATCACTGAAAATGAAAAGAAGATAGAAAGCATTCAAGGTAGTTTAAAACAACTTAAAACTGTTTATAACTCTTGTTGTGAAACTATTATGAACAACATTAATAAGCTTGATAATACGCTCCGCTTTGCAAAGAAAGAAAAAGATCCTTTACTTTTAAGCAATTTTGATAGTGTAACAGATAATGGGTTAGTAGAACCTAATCAGTTAATGGATGATTTAATAGATTTTTCCAACACTTTTGATAACATCTCCAATGAACAGTTAGATGATTTTATCTATGAAAACATGGATCGTAACATCGATTTTGAATTTGAAGGTTTTAACAATGATTTTGTTGACATTGATGCCAAAGTAATGGATTCAATGAGTGCTTTTAGTGTTAATGATTTGGATATAGAAACACTAGTTCCTGATCGTACTTCCAATTTCAGTTCATTATTAGATGAAGACTTATTTGAAAGCAGTGGTGATTTTAGCCTTGATTATTAA", "aa": "MHYESKSEPIHDYKFGSDLSQSNSNNSLESEPVKFNSETAPDAHFESQSEPVDQVQYDIYQNEELKPTLDQPSSDDYFAKQPTDENYGFDNDLPPEVKQPESVVDQPSSDDHFAKQPESTTDSYSFDSDLPQPTLDQPSLDDHVQYNFDHHEELKPVAEEQNNYQVGFDQVQANLDNNEEIQPTAEKKVTTDFESKQAQVVDSYQLPIDTDQQDQTTFSSSFETQPTVEQFDQVNSEFNDQFKSEITKEPVLESSFNKQDVVETSNYTNNLQKFDIQSDNKITITTKKSSPQIPTTLPISFVSNRIEYKPVETLALDNKESQQEQITINSITEDSKTLAKTLSVQLQQINSLNNQSIVTSESVRLDKKDDQLTINTVNSEDQQPKIEVFVKAKEPVEEHSITQNKQSVEDKSELDNFNKKSDLYKIISELKRGELNPTINFDAIFQMNDYQMSVKQSFIHLNDFVTNYKNQISERYLIIKKELQSELSRLIDQNENLNVQFNNAKNLTTLQKEEMIRSLASDFAIAYKPSNSYEQLQKSGEIMRHVQRAITENEKKIESIQGSLKQLKTVYNSCCETIMNNINKLDNTLRFAKKEKDPLLLSNFDSVTDNGLVEPNQLMDDLIDFSNTFDNISNEQLDDFIYENMDRNIDFEFEGFNNDFVDIDAKVMDSMSAFSVNDLDIETLVPDRTSNFSSLLDEDLFESSGDFSLDY", "aa_hexdigest": "84e8cb86c6668e00fb0016bd18b1ed78", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 81681.13960000037, "isoelectric_point": 4.294720268249511 }, "id": "JPCNHPFPBM_716", "locus": "GCA000292505_00701" }, { "type": "cds", "sequence": "contig_1", "start": 390704, "stop": 391045, "strand": "-", "frame": 3, "gene": null, "product": "EAGR box-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B418BE", "UniRef:UniRef90_UPI0001B418BE" ], "nt": "ATGGCTAAAAACAAGCAATCGGTTTTTGAAGAAAAAAACTATACCCAAACCGAACCTGAAAACATTTTTGGTGATCTTTATGATGGTAAATCCACTGTTGAAGAAGACCCTAATATTAAGGTAGCATATGATGCTGATGGTAATGGCTATTATATAGCTTTTAACAAAGAAACAGGGGTTTACTATGATCCTTATGGAGATACTGAATATGATATCTCCCAACTCTTTGATGAAAATGGGAATCCGTTTGTTTTTGATGAAAAGCAAGAAGAGAATGATTATTTAAAGTATGTTGGTAACCCTGACTATGGTTCTTATGATGAAAATGGTGAGTGGGTTTGA", "aa": "MAKNKQSVFEEKNYTQTEPENIFGDLYDGKSTVEEDPNIKVAYDADGNGYYIAFNKETGVYYDPYGDTEYDISQLFDENGNPFVFDEKQEENDYLKYVGNPDYGSYDENGEWV", "aa_hexdigest": "a6cebfdf4836e9cf17baf04b409741cd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 390605, "stop": 391045, "observations": { "mutation": [ 390706 ], "stop": [ 390706 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001B418BE", "query_cov": 1.0, "subject_cov": 0.6420454545454546, "identity": 1.0, "score": 236.0, "evalue": 5.35e-78, "gene_start": 1, "gene_end": 113, "reference_start": 1, "reference_end": 113, "reference_sequence": "MAKNKQSVFEEKNYTQTEPENIFGDLYDGKSTVEEDPNIKVAYDADGNGYYIAFNKETGVYYDPYGDTEYDISQLFDENGNPFVFDEKQEENDYLKYVGNPDYGSYDENGEWVWSGYFENDQWISTKESQPTDENYGFDSDLPPEVKQPESVEDNYGFDNDLPPEVKQPESVVDNY" }, "paralog": false, "identity": 0.9931506849315068, "score": 312.0, "evalue": 1.11e-110, "description": "Internal stop codon at 390706. Nonsense mutation around 390706.", "psc": { "uniref90_id": "UniRef90_UPI0001B418BE", "product": "EAGR box-containing protein", "uniref50_id": "UniRef50_UPI0001B418BE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001B418BE", "UniRef:UniRef50_UPI0001B418BE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001B418BE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B418BE" ], "product": "EAGR box-containing protein" } }, "id": "JPCNHPFPBM_717", "locus": "GCA000292505_00702" }, { "type": "cds", "sequence": "contig_1", "start": 391105, "stop": 391818, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MG313", "db_xrefs": [ "GO:0022857", "SO:0001217", "UniRef:UniRef50_Q50364", "UniRef:UniRef90_Q49414" ], "nt": "TTGTTCGGATGGATCTTTGGTCCTATTTGGGGTTTTATTTTTGGATTTTTATCTGATACTATTGATTGGTTAACAAAGGGTTATGTGTGGTTTTGGATGTTTGCATTGCAAAAGCCACTCTTTGCTTTTCTTGCTGGCATAGTTAAAGGGATTTATCTAGTTAGAAAAAATGCCAGTAATTACAAGGTTGATTTTTGGGTGTTACAAACACTATTAGTAACTTTTTTTGTTGTTAGTGTAACACTGCTTTTACTTTATCTAACTAATAATGAGTTTCAGCAAATTGGAACAAAAAATTTTCAAACTGCTGATCTTAGTGTTAATGTTGTTGTTTTACAAATCATAACTTTAGTTAGCTTTGTTATTTTCTTTTTAGTAACAGAAGGATTTTTAGGGTTTGTTTATGTAAAAAAGAGAAATAAAGAACAAGCACTTTTAACTATTTATAGCTTGGTTCTAATGGTTTTAATGTCAGTAGTTGTTTCTATCCTTTTGGGGACAATTGCTGCCATTGAGTTTATTACTTTTATTAATAATGGCAGACCAAGTAATAACTTTGTTTTGTATGGGGTTTATTTCTTTCTTTTACCAAGAGTATTGGTGCAAGCGTTATTATTACCTATCTATGTAGCTTTGTTTTACCCTTTAATAGGGATTGTGGAAAATAATTTAAAAAATTATTTATTACTATTTACGCTTTCATGGAAGAGTTAG", "aa": "MFGWIFGPIWGFIFGFLSDTIDWLTKGYVWFWMFALQKPLFAFLAGIVKGIYLVRKNASNYKVDFWVLQTLLVTFFVVSVTLLLLYLTNNEFQQIGTKNFQTADLSVNVVVLQIITLVSFVIFFLVTEGFLGFVYVKKRNKEQALLTIYSLVLMVLMSVVVSILLGTIAAIEFITFINNGRPSNNFVLYGVYFFLLPRVLVQALLLPIYVALFYPLIGIVENNLKNYLLLFTLSWKS", "aa_hexdigest": "42a2eb2c5f01879fe6485caf3da6f1cb", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q49414", "query_cov": 1.0, "subject_cov": 0.8257839721254355, "identity": 0.97, "score": 423.0, "evalue": 2.55e-148, "valid": true, "product": "Uncharacterized protein MG313", "uniref50_id": "UniRef50_Q50364", "go_ids": [ "GO:0022857" ] }, "pscc": { "uniref50_id": "UniRef50_Q50364", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50364" ], "product": "Uncharacterized protein MG313 homolog" }, "genes": [], "id": "JPCNHPFPBM_718", "locus": "GCA000292505_00703" }, { "type": "cds", "sequence": "contig_1", "start": 391844, "stop": 391957, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAATACATTAGTAACACTAATTGAAAAGATAGAAAAGATAAAGGTTAGTGCTAATAAAACACTAGCTCAAACTAACAGTTGTAAACTCTTTAAAAGTTTCAACGGATAG", "aa": "MLNTLVTLIEKIEKIKVSANKTLAQTNSCKLFKSFNG", "aa_hexdigest": "e162a67b8f6d3380cc94e925f57afdf4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4126.8806, "isoelectric_point": 9.748022651672361 }, "id": "JPCNHPFPBM_719", "locus": "GCA000292505_00704" }, { "type": "cds", "sequence": "contig_1", "start": 392013, "stop": 392201, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGTTTTTAGCATTGTTCCTTTTAGCATCATTAGTGTAATTACTTTTTCATTAAGTCCTAAATTAAACTTAGAACAAACTAATGAGATATTTAATAAGTACTTTCAAGAGGAATTGGCAAAACCAATTACTAGTGATGAAAGTAAAACTACTTTAGATATCCCCCCACCTACTATTTTTGGTTAA", "aa": "MFVFSIVPFSIISVITFSLSPKLNLEQTNEIFNKYFQEELAKPITSDESKTTLDIPPPTIFG", "aa_hexdigest": "64eff7513ca96c95099a908256bd1aac", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7025.028, "isoelectric_point": 4.4943956375122065 }, "id": "JPCNHPFPBM_720", "locus": "GCA000292505_00705" }, { "type": "cds", "sequence": "contig_1", "start": 392292, "stop": 392726, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGCCATCTTTTTCATTTCGATAGCATACGTTGTGATGGTCAATGTCTTTTTTACCCACTTTGTTGATAAGAACAGTCCACTTTTCAATGTTAATAATGACCAAAATACTGTTCAGCAAAGTAATGCAGATACAACAAATCTAAACCGCTTTTTTGCTTTAAGTTCATCTAACTTGATTACCATGATCTTCCTGGCTTTTGGTGGTGCTTCTTTTTTCTTTGCTTTTCAGGGTAAACCCTATAGCTTTGCAACTAGAGGTCAAACCAATAGAGCAATGCGGTATTTACGTAGTGAATTTGGGGTCAAAGAGTTTCCCAAAATTAATGATAACTACCGTTATAAGGTAAGAATAAAATGGATCTTTTGGACTATCTTTATCTTTGTCTTTTTAAATTGTATCCCTGGTAATATAGTAAGAAGTGGCTTTTGA", "aa": "MIAIFFISIAYVVMVNVFFTHFVDKNSPLFNVNNDQNTVQQSNADTTNLNRFFALSSSNLITMIFLAFGGASFFFAFQGKPYSFATRGQTNRAMRYLRSEFGVKEFPKINDNYRYKVRIKWIFWTIFIFVFLNCIPGNIVRSGF", "aa_hexdigest": "7ff1363e8eec5e165fb015a5add2ab4e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16759.191099999993, "isoelectric_point": 10.076618003845212 }, "id": "JPCNHPFPBM_721", "locus": "GCA000292505_00706" }, { "type": "cds", "sequence": "contig_1", "start": 392751, "stop": 393344, "strand": "-", "frame": 2, "gene": null, "product": "MPN449 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50363", "UniRef:UniRef90_Q49415" ], "nt": "ATGACGTTTAGTGAAATTCTAACTAAGGTACAAAATAATCTTGACATTGTTTTTAATAACGAAACTTTAAGAACAAGGATAAAAACTGATAGTGATTTTGCCAAAACAATTTTGGCACAACTCAAATTACTTTATTTTTTAGAGGAAAAACAAAAAAGAGTTAAAACAAAAAAACCAGACCACTTTCTTTTTGGTAGTTTTCATGATAAGTTTATCCAATTAGGGCAAAACCAATTAAGTGAAAAAGAACTTAAAGCTGCTAAGTTTGATCTAACTGATGCGTTAGATTTAGCTAATTATCTTAATGTTGCTGTTAAAAACTTGTTTAATAAAGAGTTGAATAGCTTTACCAAACTTGCTGAAACTCAAGTTAAACCCGTTAGTGAGTTACAAGAAAACAATAAAACAGTTAAAGATAACCCAAGTTTTCAAACTATAAACAATTCTCAACAGCTAAACTCAGGACTTGAGAATAATATTTTGCAACAAACCCTCCAAGTTCGTGCCAGAGATCGTGCCTTTGGTAGGTTTACCTCTGAAAAACTAGTTGGTAAGATCTTTGAGTTTCAGTTTAAGAGTCAGTGGATTAAATGA", "aa": "MTFSEILTKVQNNLDIVFNNETLRTRIKTDSDFAKTILAQLKLLYFLEEKQKRVKTKKPDHFLFGSFHDKFIQLGQNQLSEKELKAAKFDLTDALDLANYLNVAVKNLFNKELNSFTKLAETQVKPVSELQENNKTVKDNPSFQTINNSQQLNSGLENNILQQTLQVRARDRAFGRFTSEKLVGKIFEFQFKSQWIK", "aa_hexdigest": "7975f1c4f06773e25fcd0ddce6176951", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 392448, "stop": 393344, "observations": { "mutation": [ 392753 ], "stop": [ 392753 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49415", "query_cov": 1.0, "subject_cov": 0.44469525959367945, "identity": 1.0, "score": 375.0, "evalue": 7.48e-128, "gene_start": 1, "gene_end": 197, "reference_start": 1, "reference_end": 197, "reference_sequence": "MTFSEILTKVQNNLDIVFNNETLRTRIKTDSDFAKTILAQLKLLYFLEEKQKRVKTKKPDHFLFGSFHDKFIQLGQNQLSEKELKAAKFDLTDALDLANYLNVAVKNLFNKELNSFTKLAETQVKPVSELQENNKTVKDNPSFQTINNSQQLNSGLENNILQQTLQVRARDRAFGRFTSEKLVGKIFEFQFKSQWIKWAQLAIFISMIAIFFISIAYVVMVNVFFTHFVDKNSPLFNVNNDQNTVQQSNADTTNLNRFFALSSSNLITMIFLAFGGASFFFAFQGKPYSFATRGQTNRAMRYLRSEFGVKEFPKINDNYRYKVRIKWIFWTIFIFVFLNCIPGNIVRSGFWNAALIIRLFSENQLISVSPLFASYLIGIAWLFVFSIVPFSIISVIAFSLSPKLNLEQTNEIFNKYFQEELAKPITSDESKTTLDIPPPTIFG" }, "paralog": false, "identity": 0.9966442953020134, "score": 579.0, "evalue": 1.54e-210, "description": "Internal stop codon at 392753. Nonsense mutation around 392753.", "psc": { "uniref90_id": "UniRef90_Q49415", "product": "MPN449 family protein", "uniref50_id": "UniRef50_Q50363", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49415", "UniRef:UniRef50_Q50363" ] }, "pscc": { "uniref50_id": "UniRef50_Q50363", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50363" ], "product": "Uncharacterized protein MG314 homolog" } }, "id": "JPCNHPFPBM_722", "locus": "GCA000292505_00707" }, { "type": "cds", "sequence": "contig_1", "start": 393368, "stop": 393463, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATTCAAAAAATTGGATATTAAATTAAAGCACAATGATGGCTTTGCCATTCCGTTGTTAAAAAACTTTGCTTTAAGCTTTTTTACTAACTAA", "aa": "MQFKKLDIKLKHNDGFAIPLLKNFALSFFTN", "aa_hexdigest": "202f0704be188f27f7b0039db70c62ba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3640.2996000000003, "isoelectric_point": 9.82448215484619 }, "id": "JPCNHPFPBM_723", "locus": "GCA000292505_00708" }, { "type": "cds", "sequence": "contig_1", "start": 393438, "stop": 393833, "strand": "-", "frame": 2, "gene": "holA", "product": "DNA polymerase III subunit delta", "db_xrefs": [ "COG:COG1466", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0006261", "GO:0009360", "SO:0001217", "UniRef:UniRef50_Q49416", "UniRef:UniRef90_Q49416" ], "nt": "ATGGGGGTAATTTACCAAGAAATTAACAAACTTAGCTTGTTAGGTAAAAATGAAATCAAAGATAACAAATTAGTTGAAACAGTTATTTGTGATTATCAACCTGTTCAAATTTACAAATTAACCAAAGCTCTAACAAATTCACAAATTGTAAAGGCATTTAAATATATTGATGAATTGGCAAGTACAAAACCAAATTTTGCCATCCAATTTTTAGAATTTTTTAGTGGGGAGTTGTTATTAGCATTGATGGTTAAAAGTTGTAATCCAAAGCAACTAACTAACATTAATCTAAATGTTAATCAGTTTCGCTTAATTGCTATTCAAAGTCAGTACCATAACTTTACTAGCAAAGTTTTAGTAAATATAATTAATGCAATTCAAAAAATTGGATATTAA", "aa": "MGVIYQEINKLSLLGKNEIKDNKLVETVICDYQPVQIYKLTKALTNSQIVKAFKYIDELASTKPNFAIQFLEFFSGELLLALMVKSCNPKQLTNINLNVNQFRLIAIQSQYHNFTSKVLVNIINAIQKIGY", "aa_hexdigest": "280b32a4e1edc4596fd90de15e18dd62", "start_type": "ATG", "rbs_motif": null, "genes": [ "holA" ], "truncated": "both", "pseudogene": { "start": 393368, "stop": 394133, "observations": { "insertion": [ 393452 ], "mutation": [ 393860, 393935 ], "stop": [ 393860, 393935 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49416", "query_cov": 0.9847328244274809, "subject_cov": 0.43434343434343436, "identity": 0.9840000000000001, "score": 249.0, "evalue": 2.76e-81, "gene_start": 1, "gene_end": 129, "reference_start": 144, "reference_end": 272, "reference_sequence": "MTIIYGQDIGLIHQKLSQIKSNSPYKTIWFKDLKQLYDLFSQPLFGSNNEKFIVNNCSFLEKTSLTRQENLCLEKLKTTDVVLTVYTDNPFSGIKTIKSITTVFCDKLDWKSMHKAIGEVCRELNLKLDLEIIDWLANALPLNMGVIYQEINKLSLLGKNEIKDNKLVETVICDYQPVQIYKLTKALTNSQIVKAFKYIDELASTKPNFATQFLEFFSGELLLALMVKSCNPKQLTNINLNVNQFRLIAIQSQYHNFTSKVLVNIINAIQKLDIKLKHNDGFAIPLLKNFALSFFTN" }, "paralog": false, "identity": 0.9881889763779528, "score": 485.0, "evalue": 1.14e-176, "description": "Internal stop codon at 393860, 393935. Frameshift due to insertion around 393452. Nonsense mutation around 393860, 393935.", "psc": { "uniref90_id": "UniRef90_Q49416", "gene": "holA", "product": "DNA polymerase III subunit delta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_Q49416", "cog_id": "COG1466", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0006260", "GO:0006261", "GO:0009360" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49416", "EC:2.7.7.7", "UniRef:UniRef50_Q49416", "COG:COG1466", "COG:L", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0006261", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_Q49416", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49416" ], "product": "Probable DNA polymerase III subunit delta" } }, "id": "JPCNHPFPBM_724", "locus": "GCA000292505_00709" }, { "type": "cds", "sequence": "contig_1", "start": 393933, "stop": 394262, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAATAATTTATGGTCAAGATATTGGGCTAATTCACCAAAAATTAAGTCAAATCAAAAGCAATTCTCCATATAAAACTATTTGGTTTAAAGATCTTAAGCAACTTTATGATCTCTTTTCACAACCATTATTTGGTTCTAATAATGAAAAGTTTATTGTCAATAATTGCAGTTTTTTGGAAAAAACAAGCTTAACTAGACAAGAAAACTTGTGTTTAGAAAAACTAAAAACAACTGATGTAGTTTTAACTGTTTATACTGACAATCCTTTTAGTGGGATTAAAACCATTAAATCAATCACAACTGTTTTTTGTGATAAATTGGATTGA", "aa": "MTIIYGQDIGLIHQKLSQIKSNSPYKTIWFKDLKQLYDLFSQPLFGSNNEKFIVNNCSFLEKTSLTRQENLCLEKLKTTDVVLTVYTDNPFSGIKTIKSITTVFCDKLD", "aa_hexdigest": "dd63d7d34df4e7b189e0a0c82e03bf02", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12562.38229999998, "isoelectric_point": 8.422810554504398 }, "id": "JPCNHPFPBM_725", "locus": "GCA000292505_00710" }, { "type": "cds", "sequence": "contig_1", "start": 394450, "stop": 394821, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTACCTGTTTTTAGTAGGATTTGCTTTTTCAGCACTAAGAGTTTTTATCTCAACACTGTTAAAACAGGTTTTTAAAAAACAGTTACCAGAAGATAACTTAAGTTTAACTGCACTTTTAATCATTCTTATTAGTAACCATGCTTTAAATAACTTTGGTTTTAATTTTTCTTTTCTAGCTTGTTTTGTATTGTTATTTGTCAACAAACTAAAGTTATTAAAAGCTTTAAAACCGCTTGTCAGTTCTAGTTTAATCTTGATTGTTATCAGCCCCTTAAGCTTGTATTTAAATTCAAGACTAAATGCTTTTAGTGTCTTGTTTAACCTTTTGTTTAGCCCAATTGCCTTATTTTATTTTTGTGTTAGTTGA", "aa": "MIYLFLVGFAFSALRVFISTLLKQVFKKQLPEDNLSLTALLIILISNHALNNFGFNFSFLACFVLLFVNKLKLLKALKPLVSSSLILIVISPLSLYLNSRLNAFSVLFNLLFSPIALFYFCVS", "aa_hexdigest": "9a7bc2cd2c7f55cedc503890d5f89855", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13871.699499999977, "isoelectric_point": 9.864645957946777 }, "id": "JPCNHPFPBM_726", "locus": "GCA000292505_00711" }, { "type": "cds", "sequence": "contig_1", "start": 395351, "stop": 395953, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAACTTCAGGTTTACAAATTAAAGTTGTACCAAGATCAGCAGCTTCACTTCAATCAAAATTAGACACTGGTTTTCAACCTAGACAAGTTGAACGCACAACTGATTCAGATATCACTGTTAATGTCTCTTCCCACGCTTCTCTTCTAGAGAAAATTAATGCTTTAAACCACCAAAGGATAATGAATGATATTGCTCTAAAGAGTGATAACACTATAAAAAGTAGCAATTTCAGCCGTTTTTATCCTGAAAATGAGTATGTTGCAACTAAATACAGCGATCCACTTTACAGTGATGCCAATCAAAGTTTAACTAGTGATCGTTTCTCTCTTGATTTTGACTACACACCAAAATCTAGAGTCAACAATTACACACCATTAAGACCAACTAATTTCCAAAATAACGCTATTTCTAACTATCGTTTTAGCAGAACACCAAGTAGTTATTATCCCTTAACAAGAAGACCATTAAGATTAACTAATATCAGTTCATACCGTTCATCATTCCATTCACCAACAAGACTATCAAGTTTTAGAAGAACTAGTTTACCATTTAGTTCTAGCTATGATGGACTTAGACGTTATCCATCAAGATCATATTGA", "aa": "MPTSGLQIKVVPRSAASLQSKLDTGFQPRQVERTTDSDITVNVSSHASLLEKINALNHQRIMNDIALKSDNTIKSSNFSRFYPENEYVATKYSDPLYSDANQSLTSDRFSLDFDYTPKSRVNNYTPLRPTNFQNNAISNYRFSRTPSSYYPLTRRPLRLTNISSYRSSFHSPTRLSSFRRTSLPFSSSYDGLRRYPSRSY", "aa_hexdigest": "54f4de2a69ae710f470efa80d46c8db4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22984.21050000001, "isoelectric_point": 10.06707668304443 }, "id": "JPCNHPFPBM_727", "locus": "GCA000292505_00712" }, { "type": "cds", "sequence": "contig_1", "start": 395993, "stop": 397135, "strand": "-", "frame": 3, "gene": "hmw3", "product": "Cytadherence high molecular weight protein 3", "db_xrefs": [ "GO:0005886", "GO:0020035", "GO:0033111", "GO:0042995", "SO:0001217", "UniRef:UniRef50_Q57081", "UniRef:UniRef90_Q57081" ], "nt": "ATGAACGATAAACAGAAAGCTAAAATAAACAAAGCCTATAACAAGCTTTTAAAAAAGATTAACAAAAGATATCCTGATGTTAGTGTTGTTTATGCCCGTGATCATAAAAATAAGGTTCATGCTCTATACCAAGATCCTGAATCAGGTAATATCTTCTCTTTAGAAAAAAGAAAGCAGTTAGCTAGTAACTATCCTTTGTTTGAATTAACTTCAGATAACCCTATTAGCTTTACAAATAACATTGTTAGCTTAAATGCTTATGATGATAAGAACAACTTGGTAACTGTTCAATATGATCAGGATAACAACACTTTCTATGACCAAAATGGTAATGTTTTGGATGTTTCTAGCTATACTGATGAGAAAAAGGTCCCTTTAATTAACTATTTAAGCAGTACTCAAACTTCTCAAGAACAACCAACCCAACAAGATTATCCTAGTATTGATGCAGGTCTTCCTAAGATAGAAGTTGATGACCAACCAAAAGCAGCTCAACACACTACTCTAGAAACTGAAAGTGAACCTGATGTCTTTGAATTAAATGACAGTTTAAATCAACCCCAACAACCTACAGAAAACTTGGGTGATGACCAGTTTGTTGAAAAAGAAGTGCCACCTACTCAACAGCTCCATCAGGATTTAGTCCACCAACAACCCGTTCAAGTTGATAGTGGATCTCAAAACCATAGTTTTAATAATTCCCCTTCTTTAAAACCACCACTAGTTAATAAACCAGCAAAGTTAGTTCAACCTGAAGTAAAACATATTCCTCAAGTTGAAGTTCAACCGAAACCTCAAATAGTTGAACCCAAAATTGAACCAAAACCAGAAGTGAAACATGTATCACATGTTGAAATTCAACCAAAACCAGAAGTGAAACCTGTTGTTGATTCAGTACCAGAAGTTAAACAACCTGAAGTAAAACATGTACCACATGTTGAAGTTCAACCAAAACCTGTTGTTGATTTAAAACCTCAAAGGATTGAACCAAGAATTGAATCAAAACCAGAAGTAATAAAACATATTCCTCAAGTTGAAGTTCAACTAAAACCTCAAATGGTTGAACCAAGAATTGAACCAAAACCTGAAACAAAATACATCCCTCAAGTAGAATCAACTCCTCAATTTGAAGTTCACCACTGA", "aa": "MNDKQKAKINKAYNKLLKKINKRYPDVSVVYARDHKNKVHALYQDPESGNIFSLEKRKQLASNYPLFELTSDNPISFTNNIVSLNAYDDKNNLVTVQYDQDNNTFYDQNGNVLDVSSYTDEKKVPLINYLSSTQTSQEQPTQQDYPSIDAGLPKIEVDDQPKAAQHTTLETESEPDVFELNDSLNQPQQPTENLGDDQFVEKEVPPTQQLHQDLVHQQPVQVDSGSQNHSFNNSPSLKPPLVNKPAKLVQPEVKHIPQVEVQPKPQIVEPKIEPKPEVKHVSHVEIQPKPEVKPVVDSVPEVKQPEVKHVPHVEVQPKPVVDLKPQRIEPRIESKPEVIKHIPQVEVQLKPQMVEPRIEPKPETKYIPQVESTPQFEVHH", "aa_hexdigest": "bda0324a23b6617909bc31607812c16f", "start_type": "ATG", "rbs_motif": null, "genes": [ "hmw3" ], "truncated": "5-prime", "pseudogene": { "start": 395690, "stop": 397135, "observations": { "mutation": [ 395995 ], "stop": [ 395995 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q57081", "query_cov": 1.0, "subject_cov": 0.6343906510851419, "identity": 0.95, "score": 596.0, "evalue": 2.23e-209, "gene_start": 1, "gene_end": 380, "reference_start": 1, "reference_end": 380, "reference_sequence": "MNDKQKAKINKAYNKLLKKINKRYPDVSVVYARDHKNKVHALYQDPESGNIFSLEKRKQLASNYPLFELTSDNPISFTNNIVSLNAYDDKNNLVTVQYDQDNNTFYDQNGNVLDVSSYTDEKKVPLINYLSSTQTSQEQPTQQDYPSIDAGLPKIEVDDQPKAAQHTTLETESEPDVFELNDSLNQPQQPTENLGDDQFVEKEVPPTQQLHQDLVHQQPVQVDSGSQNHSFNNSPSLKPPLVNKPAKLVQPEVKHIPQVEVQPKPQIVEPKIEPKPEVKHVSHVEIQPKPEVKPVVDSVPEVKQPEVKHVPHVEVQPKPVVDLKPQRIEPRIESKPEVIKHIPQVEVQPKAQMVEPRIEPKPETKYIPQVESTPQVEVHHWKPEVKTEYQPQQPLPTSGLQIKVVPRSAALLQSKLDTGFQPRQVERTTDSDITVSVSSHASLLEKINALNHQRIMSDIALKSDNTIKSSNFSRFYPENEYVATKYSDPLYSDTNQSLTSDRFSLDFDYTPKSRVNNYTPLRSTNFQNNAISNYRFSRTPSSYYPLTRRPWRLTNISSYRSSFHSPTRLSSFRRTSLPFSSSYDGLRRYPSRSYWSKDF" }, "paralog": false, "identity": 0.9521829521829522, "score": 816.0, "evalue": 1.13e-298, "description": "Internal stop codon at 395995. Nonsense mutation around 395995.", "psc": { "uniref90_id": "UniRef90_Q57081", "gene": "hmw3", "product": "Cytadherence high molecular weight protein 3", "uniref50_id": "UniRef50_Q57081", "go_ids": [ "GO:0033111", "GO:0042995", "GO:0005886", "GO:0020035" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q57081", "UniRef:UniRef50_Q57081", "GO:0033111", "GO:0042995", "GO:0005886", "GO:0020035" ] }, "pscc": { "uniref50_id": "UniRef50_Q57081", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q57081" ], "product": "Cytadherence high molecular weight protein 3" } }, "id": "JPCNHPFPBM_728", "locus": "GCA000292505_00713" }, { "type": "cds", "sequence": "contig_1", "start": 397150, "stop": 397494, "strand": "-", "frame": 1, "gene": null, "product": "P32 adhesin", "db_xrefs": [ "GO:0005886", "GO:0020035", "GO:0033111", "GO:0042995", "SO:0001217", "UniRef:UniRef50_Q49417", "UniRef:UniRef90_Q49417" ], "nt": "ATGCAACAACGCCCTGGTTTTAACCAACCAAACCAACAGTTCCAACCACACAATAATTTCAACCCAAGGATGAACCCAAACATGCAACGCCCTGGGTTTAATCCAAACATGCAACAACGCCCTGGTTTTAACCAACCAAACCAACAGTTCCAACCACACAATAATTTCAACCCAACGATGAACCCAAACATGCAACGCCCTGGTTTTAACCAACCCCATCCTAACCAATTTGCACAACCAAATAACTTTAATCCCCAAATGCAACAACGCCCTGGGTTTAATCCAAATATGCAACGACGTTCTAACCCATCACAATTGATGCCAAAAGGCGGTTTAAAACCCTAA", "aa": "MQQRPGFNQPNQQFQPHNNFNPRMNPNMQRPGFNPNMQQRPGFNQPNQQFQPHNNFNPTMNPNMQRPGFNQPHPNQFAQPNNFNPQMQQRPGFNPNMQRRSNPSQLMPKGGLKP", "aa_hexdigest": "8e00ddfd6ba8db6e4620b8410194e294", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 397150, "stop": 397794, "observations": { "mutation": [ 397791 ], "stop": [ 397791 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49417", "query_cov": 1.0, "subject_cov": 0.40714285714285714, "identity": 0.877, "score": 101.0, "evalue": 5.51e-24, "gene_start": 1, "gene_end": 114, "reference_start": 167, "reference_end": 280, "reference_sequence": "MELNGFLRYKKLFIVLALLFTTILIVSLSLLAFALVVKTNGSELGVVFHQTEDNTTVIQGRSIVEQPWFIPTVAGSFGFSALAIILGLAIGLPIVKRKEKRLLEEKERQEQIAEQLQRISDQQEQQTVEIDPQQSQAQPSQPQVQQPLQPQFQQRVPLLRPAFNPNMQQRPGFNQPNQQFQPHNNFNPRMNPNMQRPGFNPNMQQRPGFNQPNQQFQPHNNFNPRMNPNMQRPGFNQPHPNQFAQPNNFNPNMQQRPGFNPNMQQRPNPSQLMPKGGLKP" }, "paralog": false, "identity": 0.9626168224299065, "score": 226.0, "evalue": 4.35e-75, "description": "Internal stop codon at 397791. Nonsense mutation around 397791.", "psc": { "uniref90_id": "UniRef90_Q49417", "product": "P32 adhesin", "uniref50_id": "UniRef50_Q49417", "go_ids": [ "GO:0033111", "GO:0042995", "GO:0005886", "GO:0020035" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49417", "UniRef:UniRef50_Q49417", "GO:0033111", "GO:0042995", "GO:0005886", "GO:0020035" ] }, "pscc": { "uniref50_id": "UniRef50_Q49417", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49417" ], "product": "P32 adhesin" } }, "id": "JPCNHPFPBM_729", "locus": "GCA000292505_00714" }, { "type": "cds", "sequence": "contig_1", "start": 397506, "stop": 397742, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAGGTCTTAAAAGCGGAACACGCTGTTGAAACTGTGGTTGAAGGGGTTGTTGAACTTGGGGTTGGGAAGGCTGAGCTTGGGATTGTTGAGGATCAATTTCAACTGTTTGTTGTTCTTGTTGATCTGAGATTCTTTGTAATTGCTCAGCAATCTGTTCTTGGCGTTCCTTCTCTTCAAGTAAACGTTTTTCTTTGCGCTTTACAATTGGTAAACCTATCGCAAGTCCAAGGATAA", "aa": "MQVLKAEHAVETVVEGVVELGVGKAELGIVEDQFQLFVVLVDLRFFVIAQQSVLGVPSLQVNVFLCALQLVNLSQVQG", "aa_hexdigest": "65b61f3c5267d0fc1229655a52722c1b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8449.832299999998, "isoelectric_point": 4.375886344909667 }, "id": "JPCNHPFPBM_730", "locus": "GCA000292505_00715" }, { "type": "cds", "sequence": "contig_1", "start": 397789, "stop": 397992, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGTTAAATGGATTTTTGAGATACAAAAAACTTTTTATAGTTCTTGCTTTACTTTTTACAACAATCCTAATTGTTAGTTTATCGTTACTAGCATTTGCACTTGTTGTGAAAACTAATGGTAGTGAACTAGGGGTTGTTTTTCATCAAACAGAAGATAATACAACTGTAATTCAGGGCAGATCAATTGTTGAACAGCCCTGA", "aa": "MELNGFLRYKKLFIVLALLFTTILIVSLSLLAFALVVKTNGSELGVVFHQTEDNTTVIQGRSIVEQP", "aa_hexdigest": "9d7993b6226778def9d21e86b92e2024", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7450.733800000002, "isoelectric_point": 6.532982826232909 }, "id": "JPCNHPFPBM_731", "locus": "GCA000292505_00716" }, { "type": "cds", "sequence": "contig_1", "start": 398009, "stop": 398545, "strand": "-", "frame": 3, "gene": null, "product": "MG319/MPN454 family protein", "db_xrefs": [ "GO:0005886", "RefSeq:WP_050980908.1", "SO:0001217", "UniParc:UPI0002DBBC71", "UniRef:UniRef100_UPI0002DBBC71", "UniRef:UniRef50_P47561", "UniRef:UniRef90_P47561" ], "nt": "ATGCGCTTGTTTCGATTTCTCTTTAAGCTTTGCTTTCTTTTATTGGTATTGGTAGGCTTTGCTTATCTTTTCTTAGCTATCTTTTACTTTGGTTCTCTAAACCCATTTGAGTTAGCACAACCAATGGATGTTTTCAACAGGTTCTTTTCAAAAGAAGCGTTGGATAACATCTCTTCAAACAATGGTGCAACTGCAACTGCTCAAACAAGTAGCTTATTACAACTTTTAGAAGGTTCAAGTAATGGTTTGGATAACCGTTTTCCAACTGAAAAAAGTGCATTTTATGCTATCCCAGGATATGTAGATTTTTTGACAAATGCTAAGTTACCTGGATTTGTTGAACAGTTTACACCTTATCTAACTAAGTATGTAATTCCCCTAGGAATGGCATTTGTTAGTGGTTTAATAGGTACTTTAATTGTTAACTTCTTTCTCAATAAAATCACTCGTTCAATTAAAAGAAGAAAAAGGAACATGAAAAAGCAAGAACAAGAAGAGTATTATGATGATTCAAGATCAAGAAGAAAAAGGAACTAA", "aa": "MRLFRFLFKLCFLLLVLVGFAYLFLAIFYFGSLNPFELAQPMDVFNRFFSKEALDNISSNNGATATAQTSSLLQLLEGSSNGLDNRFPTEKSAFYAIPGYVDFLTNAKLPGFVEQFTPYLTKYVIPLGMAFVSGLIGTLIVNFFLNKITRSIKRRKRNMKKQEQEEYYDDSRSRRKRN", "aa_hexdigest": "6223d7a640c1733edf8c1cdc866ac682", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002DBBC71", "ncbi_nrp_id": "WP_050980908.1", "uniref100_id": "UniRef100_UPI0002DBBC71", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002DBBC71", "RefSeq:WP_050980908.1", "UniRef:UniRef100_UPI0002DBBC71" ] }, "ips": { "uniref100_id": "UniRef100_UPI0002DBBC71", "uniref90_id": "UniRef90_P47561" }, "psc": { "uniref90_id": "UniRef90_P47561", "product": "MG319/MPN454 family protein", "uniref50_id": "UniRef50_P47561", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47561", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47561" ], "product": "Uncharacterized protein MG319" }, "genes": [], "id": "JPCNHPFPBM_732", "locus": "GCA000292505_00717" }, { "type": "cds", "sequence": "contig_1", "start": 398754, "stop": 399005, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTCACATCTTATTATGGGCTTTTGTAATTAGTATTGTAGCGAGCTTATTGAGCTTTATTTTGAACTTTACAGTCTTTGCTTCTACAACAAACCTACTTGATAGAATTATCCCTGGCTTATCTTTAATAGTAGGGGGAATCTTTTCATTAATTTCAGTTTATTTTGTGAGTTTACAGATCCGTAATGAACAAGATTTGATTAAATACTATGAAAGTGAAGATTATGAAATGGCAAGAAGACAGAGCTGA", "aa": "MFHILLWAFVISIVASLLSFILNFTVFASTTNLLDRIIPGLSLIVGGIFSLISVYFVSLQIRNEQDLIKYYESEDYEMARRQS", "aa_hexdigest": "661d40e5410c0b23eadfa555bff4be3f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9552.070799999996, "isoelectric_point": 5.045847511291503 }, "id": "JPCNHPFPBM_733", "locus": "GCA000292505_00718" }, { "type": "cds", "sequence": "contig_1", "start": 399057, "stop": 399509, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MG320", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47562", "UniRef:UniRef90_P47562" ], "nt": "ATGATTAATTCAACAAAAGGCTATATTGATCAAAACGGTTTAACAGCAAAGCAGTTTGTACAAACAAAACAGCTTTCTGTAATCCGTTTAACTTTTATGGTAGCTGCTTTTGGGATCTTTTTTATCTTTCTTGTTGCATTAACAGTACAACAATTGCTTTCAAGAAGCACACTAATTGATTTAGCTAGTGATTTTAGAACACTTAGTACTATTGCAGTTATTACTAGTTTTGTCTCTTTAATTCTTTATTTTGTTACTGCTTTTAAATTAAGGAATCCCAACACTAGTTTGACATGGTTTTGGGCCTTAATCATTACTGATGTGATTAGTTATGGGATAACCCTTGGGATCTTATTAACTTTAGCAACAACATTTTCAAAACAAGTTAACTTTGAAGCTAATGACATTGTTTATGCTTTTTTAGGTGCTAGTTTGGTGTTTGGTTCAGTGTGA", "aa": "MINSTKGYIDQNGLTAKQFVQTKQLSVIRLTFMVAAFGIFFIFLVALTVQQLLSRSTLIDLASDFRTLSTIAVITSFVSLILYFVTAFKLRNPNTSLTWFWALIITDVISYGITLGILLTLATTFSKQVNFEANDIVYAFLGASLVFGSV", "aa_hexdigest": "803c49f298d7648966de02e408e33bad", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 398754, "stop": 399509, "observations": { "mutation": [ 399059 ], "stop": [ 399059 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47562", "query_cov": 1.0, "subject_cov": 0.5244755244755245, "identity": 0.993, "score": 272.0, "evalue": 4.62e-90, "gene_start": 1, "gene_end": 150, "reference_start": 1, "reference_end": 150, "reference_sequence": "MINSTKGYIDQNGLAAKQFVQTKQLSVIRLTFMVAAFGIFFIFLVALTVQQLLSRSTLIDLASDFRTLSTIAVITSFVSLILYFVTAFKLRNPNTSLTWFWALIITDVISYGITLGILLTLATTFSKQVNFEANDIVYAFLGASLVFGSVWGLSALPSQKRRYQQTQTLFHILLWAFVISIVASLLSFILNFTVFASTTNLLDRIIPGLSLIVGGIFSLISVYFVSLQIRNEQDLIKYYESEDYEMARRQSWRSALFFGAWLISSFMNLVYFILRIILITKNFSRV" }, "paralog": false, "identity": 0.9920318725099602, "score": 471.0, "evalue": 3.41e-171, "description": "Internal stop codon at 399059. Nonsense mutation around 399059.", "psc": { "uniref90_id": "UniRef90_P47562", "product": "Uncharacterized protein MG320", "uniref50_id": "UniRef50_P47562", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47562", "UniRef:UniRef50_P47562", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47562", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47562" ], "product": "Uncharacterized protein MG320" } }, "id": "JPCNHPFPBM_734", "locus": "GCA000292505_00719" }, { "type": "tRNA", "sequence": "contig_1", "start": 399579, "stop": 399669, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(gga)", "amino_acid": "Ser", "anti_codon": "gga", "score": 70.9, "nt": "GGATACTTACCCAAGTGGCTGAAGGGGTAGGCTTGGAAAGCTTATAGATGGGTAAAACCATGCGAGGGTTCGAATCCCTCAGTATCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 399579, 399581 ], "id": "JPCNHPFPBM_13", "locus": "GCA000292505_00720" }, { "type": "tRNA", "sequence": "contig_1", "start": 399671, "stop": 399759, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(cga)", "amino_acid": "Ser", "anti_codon": "cga", "score": 78.7, "nt": "GGAGATTTACCCAAGTGGCTGAAGGGGGCGCTCTCGAAAAGCGTTAGGTGGTTATCCACGCGTGGGTTCAAATCCCACAATCTCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 399705, 399707 ], "id": "JPCNHPFPBM_14", "locus": "GCA000292505_00721" }, { "type": "cds", "sequence": "contig_1", "start": 400015, "stop": 400185, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGCTGATAAAGTTAAAGAAGCAATTAAAGATCCTGATAAATATCTATCAAAAAGAAGCAAGATAGATGACAACAAACCAACAAATGTATCAGATATCCACATTGGTTATGAATTGAAAGATCTTTATGACAACGCTGCTCAACTTAATCGCTTTAACAGCATTTGA", "aa": "MKADKVKEAIKDPDKYLSKRSKIDDNKPTNVSDIHIGYELKDLYDNAAQLNRFNSI", "aa_hexdigest": "9ff8498a54a7baeebb4dcfe7a62b59e4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6468.222300000001, "isoelectric_point": 7.9645692825317385 }, "id": "JPCNHPFPBM_735", "locus": "GCA000292505_00722" }, { "type": "cds", "sequence": "contig_1", "start": 401608, "stop": 402630, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAAAAAAAAGATACTTGTTGCTCGGTTCTACACTGACAGTTAGTGCAGCACTAATCCTAAGTGCTTGTGCATCCTCACAGTTAAACAAAGGGGTGTTTTTCACCTCTTCTTCAGCAGATTTGTTAAAGAACAACTCTGTTCCATTGAGCATGTTTAACGTTTCCCCCACTTCATCTTTTTTTGGTAATGCTTATGCAGGGTTAACTAACTATGTTGCTACTGGTACAAACCGTGATGATGGGGTTAATGTTACTGACCAAATTAAAGAAAAATTAGTGTTAGAACTTGCTTTATCAGTTACTGGCTATAAAAAGACAACACACAGTGGTCTTAAAGGTAGAGCACAAAAAAATGGTTCAACAGGTTCTTCTAATGGATCTAGTAAGAATGATTACACTAAGTTCAATGAGTGGGATGCAATAGGCACAATTTATCGTGATAGTTCAAAGTCTATTACTGAAGATCCTAACTATCACAAGATAACCCAAGAAGCAACCAGATATGAATTTACCATTAACACTAGTTTGAACTGGGTTGATAATGCTGGTAGAGAAGTCAAACAAAACAACCAACCAGTTAAACTCTCCTCTAAAGACTTTGAAAGGGGTTTTGAGACCTATATTCTCTCTTCTAACTTAGGTTTTAACCGGAATGGTTATTTTATTGACCTAATGGGTCTTGATGTTGAAAAAACCGTTGGTATGGATAAAACCAACGGTTCTAGTGGTAACAATGGTAAAGGTATTGAGATCACTGATGAAAATTATGATGTTGAAAACTATAGAAGTGTAGATGATAACAAGTTTAATGTTTATCTAACTTCTCCTTTTCCTTTCTTTCTATCAATGATGTCAAAGGAATTCTTCTTTCCAATTCCTCACACCCATCCTAAGGTAAAAGCCCTTAAACTAGGTAAAGATTCACCGCTTAAATATAACCAAAATAACAACAGAAAGATTTTAGATCAAGCAAATACTAACTTTGATGGTATCTATGGAGGGGGTGTTAATGCTTGA", "aa": "MKLKKRYLLLGSTLTVSAALILSACASSQLNKGVFFTSSSADLLKNNSVPLSMFNVSPTSSFFGNAYAGLTNYVATGTNRDDGVNVTDQIKEKLVLELALSVTGYKKTTHSGLKGRAQKNGSTGSSNGSSKNDYTKFNEWDAIGTIYRDSSKSITEDPNYHKITQEATRYEFTINTSLNWVDNAGREVKQNNQPVKLSSKDFERGFETYILSSNLGFNRNGYFIDLMGLDVEKTVGMDKTNGSSGNNGKGIEITDENYDVENYRSVDDNKFNVYLTSPFPFFLSMMSKEFFFPIPHTHPKVKALKLGKDSPLKYNQNNNRKILDQANTNFDGIYGGGVNA", "aa_hexdigest": "38afe062f9c2d536712228a5244fa71e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 37611.541200000014, "isoelectric_point": 8.942103385925293 }, "id": "JPCNHPFPBM_736", "locus": "GCA000292505_00723" }, { "type": "tRNA", "sequence": "contig_1", "start": 402841, "stop": 402925, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(tag)", "amino_acid": "Leu", "anti_codon": "tag", "score": 64.0, "nt": "GCACTCGTGGCGGAATGGTAGACGCGCTAGACTTAGGATCTAGTTTCATCTAGAAGTGGGGGTTCAAGTCCCTTCGGGTGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 402905, 402907 ], "id": "JPCNHPFPBM_25", "locus": "GCA000292505_00724" }, { "type": "tRNA", "sequence": "contig_1", "start": 402931, "stop": 403006, "strand": "-", "gene": "trnK", "product": "tRNA-Lys(ctt)", "amino_acid": "Lys", "anti_codon": "ctt", "score": 81.4, "nt": "GCATCTTTAGCTCAGTTGGTAGAGCAAATGACTCTTAATCATTGGGTCGTGGGTTCGATCCCCTCAAGATGCACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 403000, 403002 ], "id": "JPCNHPFPBM_24", "locus": "GCA000292505_00725" }, { "type": "tRNA", "sequence": "contig_1", "start": 403013, "stop": 403088, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(tgt)", "amino_acid": "Thr", "anti_codon": "tgt", "score": 90.9, "nt": "GCCGACTTAGCTCAGTTGGTAGAGCAATTGACTTGTAATCAATAGGTCGTAGGTTCAAATCCTATAGTCGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 403053, 403055 ], "id": "JPCNHPFPBM_23", "locus": "GCA000292505_00726" }, { "type": "tRNA", "sequence": "contig_1", "start": 403090, "stop": 403165, "strand": "-", "gene": "trnV", "product": "tRNA-Val(tac)", "amino_acid": "Val", "anti_codon": "tac", "score": 81.7, "nt": "GGATTGTTAGCTCAGCTGGGAGAGCATATGCCTTACAAGCATAGGGTCGGGGGTTCGATCCCCTCACAATCCACCA", "db_xrefs": [ "SO:0000273" ], "anti_codon_pos": [ 403130, 403132 ], "id": "JPCNHPFPBM_22", "locus": "GCA000292505_00727" }, { "type": "tRNA", "sequence": "contig_1", "start": 403174, "stop": 403248, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(cgt)", "amino_acid": "Thr", "anti_codon": "cgt", "score": 80.2, "nt": "GCCGAATTAGCTCAAGGGCAGAGCGATGCACTCGTAATGCATAGGCTACAGGTTCAATTCCTGTATTCGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 403214, 403216 ], "id": "JPCNHPFPBM_21", "locus": "GCA000292505_00728" }, { "type": "tRNA", "sequence": "contig_1", "start": 403251, "stop": 403327, "strand": "-", "gene": "trnE", "product": "tRNA-Glu(ttc)", "amino_acid": "Glu", "anti_codon": "ttc", "score": 58.9, "nt": "GGCGCGTTGGTGAAGAGACTTAACACACACGCCTTTCACGCGTGCATTCACGGGTTTGAATCCCGTACGCGTCACCA", "db_xrefs": [ "SO:0000259" ], "anti_codon_pos": [ 403291, 403293 ], "id": "JPCNHPFPBM_20", "locus": "GCA000292505_00729" }, { "type": "tRNA", "sequence": "contig_1", "start": 403334, "stop": 403408, "strand": "-", "gene": "trnN", "product": "tRNA-Asn(gtt)", "amino_acid": "Asn", "anti_codon": "gtt", "score": 84.0, "nt": "GGCCACATAGCTCAGCGGTAGAGCAACCGGCTGTTAACCGGTTGGTCACAGGTTCGATCCCTGTTGTGGCCGCCA", "db_xrefs": [ "SO:0000257" ], "anti_codon_pos": [ 403374, 403376 ], "id": "JPCNHPFPBM_19", "locus": "GCA000292505_00730" }, { "type": "cds", "sequence": "contig_1", "start": 403430, "stop": 404740, "strand": "-", "frame": 3, "gene": "trkG", "product": "putative cation transporter MG322", "db_xrefs": [ "COG:COG0168", "COG:P", "GO:0005886", "GO:0008324", "GO:0030001", "SO:0001217", "UniRef:UniRef50_P75323", "UniRef:UniRef90_P47564" ], "nt": "TTGTTTAACTTTCACAAGAAGGAACAATACAGTTTTTATGAAAAGTTAATGTTGCAATCAGAACGAGGTGGTTCTAAGCTAGGTAATACTAGTGAGATGATCTTAGTATCTATCATCTTTCTGTTTATCGTTGAACTAATTTATGGATTTTTATATGGTATTTTGTTTTATTTCATCCCAGGCTTTGAACCTGCTAACTTGTTTGCAGATCATGCAAAAGTTTCAACTCAATTAAAAGCTTTAGTAGTTGATTCAAACCAAACAATAGCAGCTTTTAATGATATTAATAAGGCTTTTCAAGCAGGTTTTTTCCATTCCTTATCAGCAGTTAATAATGCTGGGATAGATCTGATAGGGGGTAGTTCTTTTGTTCCTTATAGAAATGGACTTGGTATTATTATCCAGTGGTTAACTATTAGCCAAATTATCTTTGGGGGAATTGGTTATCCTTGTTTGTTTGATGGCTTTGAAGCCATTAAAAAAAAGATTAAGTATGGTAGACACACAAAACACCAATTTAGTCTATTTACCAAGTTAACAGTAATTACTAATATCGTTGTAATCCTGCTTTTTTTCACCTTACTTTTAATGGTGGAATTTATTGCTAGTGATAGTTTAACTAACACTATTGTTAATTTTAGTGATGAAAAAAAGAGTTTAATAAATACCCAATTGCAATCACAATCTAACCAAGCAATCCATGCGTCAGTTTTTGGTAATAACCCTAATGCAAGTAGGGTAATGCAGCTCTTTTTTATGGTTATTTCATCCCGTTCAGCAGGTTTTAGTGTTTTCCCTGTTGCTAGTGAGATTCAAACTACAAAAATAATTATTGCATTGGCAATGTTTATTGGTGCTAGTCCCTCTTCTACTGCTGGGGGGATTAGAACAACTACGCTAGCAGTAATCTTTTTAGCTCTAGTTGCTAAGTTTAAAGGTCAAAAGGAAGTAAAAGCATTTAAGCGTTCAATCGATCAAACTACAGTAATAGATGCTTTTTTAGTACTAATTTTAAGTTTAATTGCAGTTTTACTAACAGCTGTTCTTCTACCTTTAAGTATGGAACAACCAGTTAGTTTCATTGATGCTTTATTTGAAACAACTAGTGCTTTTGGAACAGTTGGACTTTCAAGTGGAGCTACTGTTAACATTGCTTTAGATCCAAATAGAAATACCTTTAATTTCCTTGCTTTATGTCTATTAATGGTTATGGGACAGGTTGGTGTGTCCAGTTCTGTGCTAACTTTTGTTAGAAAACATCCCAAAGCAAATAGTTATTCATATCCTAAGGAAGCTGTTAAAATTGGCTAG", "aa": "MFNFHKKEQYSFYEKLMLQSERGGSKLGNTSEMILVSIIFLFIVELIYGFLYGILFYFIPGFEPANLFADHAKVSTQLKALVVDSNQTIAAFNDINKAFQAGFFHSLSAVNNAGIDLIGGSSFVPYRNGLGIIIQWLTISQIIFGGIGYPCLFDGFEAIKKKIKYGRHTKHQFSLFTKLTVITNIVVILLFFTLLLMVEFIASDSLTNTIVNFSDEKKSLINTQLQSQSNQAIHASVFGNNPNASRVMQLFFMVISSRSAGFSVFPVASEIQTTKIIIALAMFIGASPSSTAGGIRTTTLAVIFLALVAKFKGQKEVKAFKRSIDQTTVIDAFLVLILSLIAVLLTAVLLPLSMEQPVSFIDALFETTSAFGTVGLSSGATVNIALDPNRNTFNFLALCLLMVMGQVGVSSSVLTFVRKHPKANSYSYPKEAVKIG", "aa_hexdigest": "e90f5a6934fd9433f929ef83975f855a", "start_type": "TTG", "rbs_motif": null, "genes": [ "trkG" ], "truncated": "3-prime", "pseudogene": { "start": 403430, "stop": 405040, "observations": { "mutation": [ 404746, 404941 ], "stop": [ 404746, 404941 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47564", "query_cov": 1.0, "subject_cov": 0.7716814159292036, "identity": 0.995, "score": 808.0, "evalue": 2.04e-292, "gene_start": 1, "gene_end": 436, "reference_start": 130, "reference_end": 565, "reference_sequence": "MTRRQNQMVKLTTWLKKIGWGETITQRIFCFYIYCILFGSLLLFLPIALQDNYQKVVSYGIDWQGKRFEQKTDYNFLDALFLSTSAFSDTGLSTVVVSKTYSIFGQIVLAVLLQLGGIGFVVIAFLAWRLFNFHKKEQYSFYEKLMLQSERGGSKLGNTSEMILVSIIFLFIVELIYGFLYGILFYFIPGFEPANLFADHAKVSTQLKALVVDSNQTIAAFNDINKAFQAGFFHSLSAVNNAGIDLIGGSSFVPYRNGLGIIIQWLTISQIIFGGIGYPCLFDGFEAIKKKIKYGRHTKHQFSLFTKLTVITNIVVILLFFTLLLMVEFIASDSLTNTIVNFSDEKKSLINTQLQSQSNQAIHASVFGNNPNASRVMQLFFMVISSRSAGFSVFPVASEIQTTKIIIALAMFIGASPSSTAGGIRTTTLAVIFLALVAKFKGQKEVKAFKRSIDQTTVIDAFLVLIISLIAVLLTAVLLPLSMEQPVSFIDALFETTSAFGTVGLSSGATVNIALDPNRNTFNFLALCLLMVMGQVGVSSSVLTFVRKHPKANSYSYPKEAVKIG" }, "paralog": false, "identity": 0.9925373134328358, "score": 1020.0, "evalue": 0.0, "description": "Internal stop codon at 404746, 404941. Nonsense mutation around 404746, 404941.", "psc": { "uniref90_id": "UniRef90_P47564", "gene": "TrkG", "product": "Uncharacterized cation transporter MG322", "uniref50_id": "UniRef50_P75323", "cog_id": "COG0168", "cog_category": "P", "go_ids": [ "GO:0005886", "GO:0008324", "GO:0030001" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47564", "UniRef:UniRef50_P75323", "COG:COG0168", "COG:P", "GO:0005886", "GO:0008324", "GO:0030001" ] }, "pscc": { "uniref50_id": "UniRef50_P75323", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75323" ], "product": "Uncharacterized cation transporter MG322 homolog" } }, "id": "JPCNHPFPBM_737", "locus": "GCA000292505_00731" }, { "type": "cds", "sequence": "contig_1", "start": 405163, "stop": 405846, "strand": "+", "frame": 1, "gene": "trkA", "product": "putative protein MG323", "db_xrefs": [ "COG:COG0569", "COG:PT", "GO:0006813", "GO:0008324", "RefSeq:WP_010869432.1", "SO:0001217", "UniParc:UPI0000139619", "UniRef:UniRef100_P47565", "UniRef:UniRef50_P75322", "UniRef:UniRef90_P47565" ], "nt": "ATGAAACGTGCAGATTTTTGCATTATTGGTTTAGGTAGATTTGGGATGCAGGTTGCACAATCACTAAAAGAAAACAACTTTAACTTACTTTTAATTGATCTTGATGATAAAAAAACTGACACCGCCTCACAACAGTTTGATTATGTTATTTGTTGTGATGCTAGTAATCTAACTGCTTTAGAAGAGTTACAAATTGATGAGTTTGCTGGGGTTATTGTTGGGGTTACCAACATAGAAGCGAGCATCATGATATGTGCTAATTTAAGGGAATTAGGACAAAAAAACATTATTGCTAAAGCCAAAAATGAAGTGCATAAAAGGGTGTTAAGCACAATGGGAATTAGAGAAGCTTTGATCCCTGAAAAGATTGTTGGTAAAAATCTGGTTATCCGCTTAATCCATGGGCTTGAAAATGAAATTATCAACCTTGGTAATGAGATAATTTTCATCCGTTCAGCAGTTAACAACAAGGCTTTTTTTAACAAAAGGTTAGAAGAGATTAACTTTAGGCAAAACACCGATGCTAACATTATCTCCATCATGCGCAGTAATAAAACTGTTGTTTTTCCTTTAGGACCAAATACTGAGATCCAACCAGGGGATATTATCACTGCAGTTTGTCAACAAAAAAGTTTAAATAAGTACTTAAATTACATCAATCCTAAAACCAAAAATAAAAATTAA", "aa": "MKRADFCIIGLGRFGMQVAQSLKENNFNLLLIDLDDKKTDTASQQFDYVICCDASNLTALEELQIDEFAGVIVGVTNIEASIMICANLRELGQKNIIAKAKNEVHKRVLSTMGIREALIPEKIVGKNLVIRLIHGLENEIINLGNEIIFIRSAVNNKAFFNKRLEEINFRQNTDANIISIMRSNKTVVFPLGPNTEIQPGDIITAVCQQKSLNKYLNYINPKTKNKN", "aa_hexdigest": "bc98d52b4d6eeab8face0013d7c32f12", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139619", "ncbi_nrp_id": "WP_010869432.1", "uniref100_id": "UniRef100_P47565", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139619", "RefSeq:WP_010869432.1", "UniRef:UniRef100_P47565" ] }, "ips": { "uniref100_id": "UniRef100_P47565", "uniref90_id": "UniRef90_P47565" }, "psc": { "uniref90_id": "UniRef90_P47565", "gene": "TrkA", "product": "Uncharacterized protein MG323", "uniref50_id": "UniRef50_P75322", "cog_id": "COG0569", "cog_category": "PT", "go_ids": [ "GO:0008324", "GO:0006813" ] }, "pscc": { "uniref50_id": "UniRef50_P75322", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75322" ], "product": "Uncharacterized protein MG323 homolog" }, "genes": [ "trkA" ], "id": "JPCNHPFPBM_738", "locus": "GCA000292505_00732" }, { "type": "ncRNA", "class": [ "RNase_P_RNA", "SO:0000386" ], "sequence": "contig_1", "start": 405886, "stop": 406226, "strand": "-", "gene": "RNaseP_bact_b", "product": "Bacterial RNase P class B", "score": 251.3, "evalue": 2.2e-27, "db_xrefs": [ "RFAM:RF00011", "SO:0000386" ], "nt": "GCTGTCGGTTAGTTGCTGCTTTAAAGATATATTTAAAGTAGAGGAAAGTCCATGCTTGCACTTGCTGGAATGCAAGTAGTGTTTGTGTAAATCAAAGCGATAAGATTTAGGATGTGATAGCCATAACGACATAACTAGTCTAAGGTTAATACTATGATGATGTTTTGAAAGTGCCACAGAGACGAGCTTGGTGAAAACCAGGTATGAAACGCGGTAAACTCCACAAGCAAGAAACCTAAATTTTGGTAAGGGAATGTTGCTTTAAGAACAAATAATTAAAGTAATGACAGCAATTGCTGTAGATAAATAACTGACTTTAAAACAGAACATGGCTTATTGAC", "id": "JPCNHPFPBM_41", "locus": "GCA000292505_00733" }, { "type": "tmRNA", "sequence": "contig_1", "start": 406249, "stop": 406636, "strand": "+", "gene": "ssrA", "product": "transfer-messenger RNA, SsrA", "db_xrefs": [ "SO:0000584" ], "tag": { "start": 406332, "stop": 406412, "aa": "DKENNEVLVDPNLIINQQASVNFAFA", "nt": "GACAAAGAAAATAACGAAGTGTTGGTAGATCCAAATTTGATCATTAACCAACAAGCAAGTGTTAACTTTGCTTTTGCATAA" }, "nt": "GGGGATGTTTTGGGTTTGACATAATGCTGATAGACAAACAGTAGCATTGGGGTATGCCCCTTACAGCGCTAGGTTCAATAACCGACAAAGAAAATAACGAAGTGTTGGTAGATCCAAATTTGATCATTAACCAACAAGCAAGTGTTAACTTTGCTTTTGCATAAGTAGATACTAAAGCTACAGCTGGTGAATAGTCATAGTTTGCTAGCTGTCATAGTTTATGACTCGAGGTTAAATCGTTCAATTTAACCTTTAAAAATAGAACTTGTTGTTTCCATGATTGTTTTGTGATCAATTGGAAACAAGACAAAAATCCACAAAACTAAAATGTAGAAGCTGTTTGTTGTGTCCTTTATGGAAACGGGTTCGATTCCCGTCATCTCCACCA", "id": "JPCNHPFPBM_37", "locus": "GCA000292505_00734" }, { "type": "cds", "sequence": "contig_1", "start": 406787, "stop": 406945, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTATTTTTGGTGTTATTAGACCAACAGTTTTTTGAAAGAGATACACAAAAAATTCAATCAGCAACGCGGTTTAGCTTGTTTTTTAATGTTGAAAATAACAATGGTGTTTATCTCTCTAAATTAAATAGTTTTGGTGTATTTGCTACCGCTTGA", "aa": "MVLFLVLLDQQFFERDTQKIQSATRFSLFFNVENNNGVYLSKLNSFGVFATA", "aa_hexdigest": "e984ef0aaf7f2c60a8f119f77d0000b3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6009.7965, "isoelectric_point": 5.971413612365722 }, "id": "JPCNHPFPBM_739", "locus": "GCA000292505_00735" }, { "type": "cds", "sequence": "contig_1", "start": 407177, "stop": 407446, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTTTCTAAAAAGAAAAATAGTGGTGGTGGTTGGATTAATAAGATATCTGATAAACTCAGTGAAATAAATGTCGCTGAGTATCAAGTTGTTTCCAAACACAGTAACTATCTGTTTTACAGCAGGTTTGGCTTACTTGATACTTTTGTTTACTTTTTATTTTTTCTCTGCTTTTTTCTAAACAACGTTTTGTTTTTAGCTGGCGTTTTTCACACTAAACAATTCACTTTTAACGAAGTTAACGGCTTTAATCAGTTTTACCTTTTTTGA", "aa": "MLSKKKNSGGGWINKISDKLSEINVAEYQVVSKHSNYLFYSRFGLLDTFVYFLFFLCFFLNNVLFLAGVFHTKQFTFNEVNGFNQFYLF", "aa_hexdigest": "fd133266959697cd97b6eddd4a3a3c52", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10493.975099999987, "isoelectric_point": 9.131962776184086 }, "id": "JPCNHPFPBM_740", "locus": "GCA000292505_00736" }, { "type": "cds", "sequence": "contig_1", "start": 407466, "stop": 408113, "strand": "-", "frame": 2, "gene": "pepP", "product": "Putative Xaa-Pro aminopeptidase", "db_xrefs": [ "COG:COG0006", "COG:E", "EC:3.4.11.9", "GO:0004177", "GO:0005829", "GO:0006508", "GO:0008237", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47566", "UniRef:UniRef90_P47566" ], "nt": "TTGCCTAGTGAGATTCAAGCCATTGAAAAAGCAGTTGATATTACAAGAAAAGTAGCAGTTAAGTTAAAGCGTTTTATTAAACCAAAAATGACTGAGCTTTTCATTTCACAATGGATTACTAATGAGCTTGTGAAGCAGGGTGGGGCTAAAAACTCATTTGATCCTATAGTAGCTACTGGTAAAAACGGTGCTAACCCACATCACAAACCAACCAAAACAATAGTTAAGGAAGGTGATTTTATCACTTGTGATTTTGGCACCATTTACAACGGTTATTGCTCTGATATTACAAGAACCTTTTTAGTTGGTAAAAAACCTAAAAGTGCAAAGTTATTATCTGCATACAAAAAAGTTGAAGAGGCTAATTTGGCTGGTATAAATGCAGTAAACACTACTTTAACAGGTTCACAAGTTGATAAGGTGTGCCGCGATATTATTGAAAACTCTGAGTTTAAAGACTTTTTTGTGCATAGTACTGGTCATGGCGTTGGTATTGATATCCATGAAATGCCAAATGTTTCCCAATCTTACAACAAGTTATTGTGTGAAAATGGGGTTGTAACCATTGAACCTGGGATCTATATTCCTAACCTTGGTGGAATTAGGATAGAAGATATGGTACTTGTTAAAAAAGAAAAATCAGTTTGA", "aa": "MPSEIQAIEKAVDITRKVAVKLKRFIKPKMTELFISQWITNELVKQGGAKNSFDPIVATGKNGANPHHKPTKTIVKEGDFITCDFGTIYNGYCSDITRTFLVGKKPKSAKLLSAYKKVEEANLAGINAVNTTLTGSQVDKVCRDIIENSEFKDFFVHSTGHGVGIDIHEMPNVSQSYNKLLCENGVVTIEPGIYIPNLGGIRIEDMVLVKKEKSV", "aa_hexdigest": "c1439c6283541e84a60c4a4643c85ca5", "start_type": "TTG", "rbs_motif": null, "genes": [ "pepP" ], "truncated": "both", "pseudogene": { "start": 407436, "stop": 408413, "observations": { "mutation": [ 407468, 408176, 408401 ], "stop": [ 407468, 408176, 408401 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47566", "query_cov": 1.0, "subject_cov": 0.6073446327683616, "identity": 0.995, "score": 432.0, "evalue": 4.99e-151, "gene_start": 1, "gene_end": 215, "reference_start": 130, "reference_end": 344, "reference_sequence": "MISELQQKITVLKDLLKTNKADAILIGSDQNRFWLTNFPSSAGWLIITSNKAKLFIDGRYYEAARNFINPIVEVELFVSFKQVKAFCESNGINHLLIEGDYLTFNYQDWIQAICKQYTVINAQEIRRVKLPSEIQAIEKAVDITRKVAVKLKRFIKPKMTELFISQWITNELVKQGGAKNSFDPIVATGKNGANPHHKPTKTIVKEGDFITCDFGTIYNGYCSDITRTFLVGKKPKSAKLLSAYKKVEEANLAGINAVNTTLTGSQVDKVCRDIIENSEFKDFFVHSTGHGVGIDIHEMPNVSQSYNKLLCENGVVTIEPGIYIPNLGGIRIEDMVLVKKEKSVWLSKSIPRAF" }, "paralog": false, "identity": 0.9876923076923076, "score": 639.0, "evalue": 2.16e-235, "description": "Internal stop codon at 407468, 408176, 408401. Nonsense mutation around 407468, 408176, 408401.", "psc": { "uniref90_id": "UniRef90_P47566", "gene": "pepP", "product": "Putative Xaa-Pro aminopeptidase", "ec_ids": [ "3.4.11.9" ], "uniref50_id": "UniRef50_P47566", "cog_id": "COG0006", "cog_category": "E", "go_ids": [ "GO:0005829", "GO:0004177", "GO:0046872", "GO:0008237", "GO:0006508" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47566", "EC:3.4.11.9", "UniRef:UniRef50_P47566", "COG:COG0006", "COG:E", "GO:0005829", "GO:0004177", "GO:0046872", "GO:0008237", "GO:0006508" ] }, "pscc": { "uniref50_id": "UniRef50_P47566", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47566" ], "product": "Putative Xaa-Pro aminopeptidase" } }, "id": "JPCNHPFPBM_741", "locus": "GCA000292505_00737" }, { "type": "cds", "sequence": "contig_1", "start": 408174, "stop": 408341, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTATTGATGGTAGGTATTATGAAGCAGCAAGGAACTTTATTAATCCTATTGTTGAAGTTGAATTATTTGTTAGTTTTAAACAAGTTAAAGCTTTTTGTGAATCTAATGGTATCAATCACTTTTTAATTGAGGGTGACTATCTTACCTTTAACTATCAGGATTGA", "aa": "MFIDGRYYEAARNFINPIVEVELFVSFKQVKAFCESNGINHFLIEGDYLTFNYQD", "aa_hexdigest": "3c670bd85313417fd498eec0b990b74a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6517.268599999999, "isoelectric_point": 4.63461742401123 }, "id": "JPCNHPFPBM_742", "locus": "GCA000292505_00738" }, { "type": "cds", "sequence": "contig_1", "start": 408399, "stop": 408500, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAGTGAATTACAACAAAAAATTACAGTTCTTAAAGATCTTTTAAAAACCAATAAAGCTGATGCTATCCTAATTGGTTCTGATCAAAACCGCTTTTGA", "aa": "MISELQQKITVLKDLLKTNKADAILIGSDQNRF", "aa_hexdigest": "630404c05c9db6326146da9695555f66", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3745.3478999999998, "isoelectric_point": 8.189692878723147 }, "id": "JPCNHPFPBM_743", "locus": "GCA000292505_00739" }, { "type": "cds", "sequence": "contig_1", "start": 408500, "stop": 408661, "strand": "-", "frame": 3, "gene": "rpmG", "product": "50S ribosomal protein L33", "db_xrefs": [ "COG:COG0267", "COG:J", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_009885961.1", "SO:0001217", "UniParc:UPI0000132FB9", "UniRef:UniRef100_P47567", "UniRef:UniRef50_P47567", "UniRef:UniRef90_P47567" ], "nt": "ATGGCTGTTAAAAGAAGCACACGACTAGGTTGTAATGAATGTTCTGAGATTAATTATTTAACCTTTAAAAACGTCAAGAAAAATCCAGAAAAACTAGCACTTAATAAGTTTTGTTCACGTTGTCGTAAGGTAGTATTGCACAAAGAGGTAAAACGTAAATAA", "aa": "MAVKRSTRLGCNECSEINYLTFKNVKKNPEKLALNKFCSRCRKVVLHKEVKRK", "aa_hexdigest": "57bb09256735e72ca12fdf19d5cbdcfd", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000132FB9", "ncbi_nrp_id": "WP_009885961.1", "uniref100_id": "UniRef100_P47567", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000132FB9", "RefSeq:WP_009885961.1", "UniRef:UniRef100_P47567" ] }, "ips": { "uniref100_id": "UniRef100_P47567", "uniref90_id": "UniRef90_P47567" }, "psc": { "uniref90_id": "UniRef90_P47567", "gene": "rpmG", "product": "50S ribosomal protein L33", "uniref50_id": "UniRef50_P47567", "cog_id": "COG0267", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P47567", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47567" ], "product": "Large ribosomal subunit protein bL33A" }, "genes": [ "rpmG" ], "id": "JPCNHPFPBM_744", "locus": "GCA000292505_00740" }, { "type": "cds", "sequence": "contig_1", "start": 408678, "stop": 409172, "strand": "-", "frame": 2, "gene": "degV", "product": "DegV domain-containing protein MG326", "db_xrefs": [ "COG:COG1307", "COG:I", "GO:0008289", "SO:0001217", "UniRef:UniRef50_P75312", "UniRef:UniRef90_P47568" ], "nt": "TTGGTCGAAGATATAAAGGCATTGGTTGACAAGGGTTGTGATAATCAAACAATTAAAGCAAAGGTTGAATCACATAAACAAAATATCCTTTCTGCAGTTACTTTAAAAAACCTAGTGCAAATGCGTAAAGGTGGGAGAATCTCTGGTTTGAAAAAGTTCATTACTACTTTACTAAGAGTTAAACCTATTATCTTGTTTGACAAGGGAGTTAATACTCTAGGAGCAAAAGTTTTTAGTTTTAGTCAAGCGGTTGAGAAGATATTTGGTTTTGTTAAAACTAAGTTTGGTGATAACTACAAAATTAAACGGATCGGTTTTTGCTATTCTTTTTGTAAAAATTACGTTAATGAAATTAAAAAAATAATTACTGATTTCATTGAGCATAATAAGATTAACTTTCAAAATGAAATTGAAAATGCTTTCATTACAAGTGTGATTATTGTTCACACTGGCATTGATGCTTTTTCTATTAGTTTACTTATTGACAATAAATAA", "aa": "MVEDIKALVDKGCDNQTIKAKVESHKQNILSAVTLKNLVQMRKGGRISGLKKFITTLLRVKPIILFDKGVNTLGAKVFSFSQAVEKIFGFVKTKFGDNYKIKRIGFCYSFCKNYVNEIKKIITDFIEHNKINFQNEIENAFITSVIIVHTGIDAFSISLLIDNK", "aa_hexdigest": "20fae5cc935f97a6960a21ad900a20e1", "start_type": "TTG", "rbs_motif": null, "genes": [ "degV" ], "truncated": "3-prime", "pseudogene": { "start": 408678, "stop": 409472, "observations": { "mutation": [ 409175 ], "stop": [ 409175 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47568", "query_cov": 1.0, "subject_cov": 0.5559322033898305, "identity": 0.988, "score": 317.0, "evalue": 2.56e-107, "gene_start": 1, "gene_end": 164, "reference_start": 132, "reference_end": 295, "reference_sequence": "MKKTAIITDSTASIKPGEINGVYILPLQVIVDGEKSFRDGIEIDYDHVHKLLKENPHGLNISTSLPRQSDLLKIFEEIKTKYDRFIFLPLSKGLSGTYDMLVQLAKELSEQNKDKEFLVFETSDIAISLKWLVEDIKALVDKGCDNQTIKAKVESHKQNILSAVTLKNLVQMRKGGRISGLKKFITTLLRVKPIILFDKGVNTLGAKVFSFSQAVEKIFGFVKTKFGDNYKIKRIGFCYSFCKNYANEIKKIITDFIEHNKINFQNEIENAFITSVIIVHTGIDAFSISLLIDNK" }, "paralog": false, "identity": 0.9924242424242424, "score": 514.0, "evalue": 1.03e-187, "description": "Internal stop codon at 409175. Nonsense mutation around 409175.", "psc": { "uniref90_id": "UniRef90_P47568", "gene": "DegV", "product": "DegV domain-containing protein MG326", "uniref50_id": "UniRef50_P75312", "cog_id": "COG1307", "cog_category": "I", "go_ids": [ "GO:0008289" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47568", "UniRef:UniRef50_P75312", "COG:COG1307", "COG:I", "GO:0008289" ] }, "pscc": { "uniref50_id": "UniRef50_P75312", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75312" ], "product": "DegV domain-containing protein MG326 homolog" } }, "id": "JPCNHPFPBM_745", "locus": "GCA000292505_00741" }, { "type": "cds", "sequence": "contig_1", "start": 409173, "stop": 409565, "strand": "-", "frame": 2, "gene": "degV", "product": "DegV domain-containing protein MG326", "db_xrefs": [ "COG:COG1307", "COG:I", "GO:0008289", "SO:0001217", "UniRef:UniRef50_P75312", "UniRef:UniRef90_P47568" ], "nt": "ATGAAGAAAACAGCTATCATCACTGATTCAACTGCTTCTATTAAACCTGGGGAGATTAACGGCGTTTACATCTTGCCTTTACAAGTAATTGTTGATGGTGAAAAAAGCTTTCGTGATGGGATAGAAATTGATTATGATCATGTTCATAAACTTCTAAAAGAAAACCCACATGGACTTAACATCTCAACTTCCTTACCACGTCAAAGTGATCTTCTTAAGATCTTTGAAGAGATTAAAACTAAGTATGATCGCTTTATTTTTCTGCCTTTAAGTAAAGGTTTAAGTGGTACTTATGATATGTTGGTTCAATTAGCAAAAGAGCTTAGTGAACAAAATAAAGACAAAGAATTTTTAGTGTTTGAAACTAGTGATATTGCTATTTCTCTAAAATGA", "aa": "MKKTAIITDSTASIKPGEINGVYILPLQVIVDGEKSFRDGIEIDYDHVHKLLKENPHGLNISTSLPRQSDLLKIFEEIKTKYDRFIFLPLSKGLSGTYDMLVQLAKELSEQNKDKEFLVFETSDIAISLK", "aa_hexdigest": "3bb653f8ccc2d08412055983b432fbcb", "start_type": "ATG", "rbs_motif": null, "genes": [ "degV" ], "truncated": "5-prime", "pseudogene": { "start": 408870, "stop": 409565, "observations": { "mutation": [ 409175 ], "stop": [ 409175 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47568", "query_cov": 1.0, "subject_cov": 0.4406779661016949, "identity": 1.0, "score": 257.0, "evalue": 2.29e-84, "gene_start": 1, "gene_end": 130, "reference_start": 1, "reference_end": 130, "reference_sequence": "MKKTAIITDSTASIKPGEINGVYILPLQVIVDGEKSFRDGIEIDYDHVHKLLKENPHGLNISTSLPRQSDLLKIFEEIKTKYDRFIFLPLSKGLSGTYDMLVQLAKELSEQNKDKEFLVFETSDIAISLKWLVEDIKALVDKGCDNQTIKAKVESHKQNILSAVTLKNLVQMRKGGRISGLKKFITTLLRVKPIILFDKGVNTLGAKVFSFSQAVEKIFGFVKTKFGDNYKIKRIGFCYSFCKNYANEIKKIITDFIEHNKINFQNEIENAFITSVIIVHTGIDAFSISLLIDNK" }, "paralog": false, "identity": 0.9956709956709957, "score": 446.0, "evalue": 2.41e-161, "description": "Internal stop codon at 409175. Nonsense mutation around 409175.", "psc": { "uniref90_id": "UniRef90_P47568", "gene": "DegV", "product": "DegV domain-containing protein MG326", "uniref50_id": "UniRef50_P75312", "cog_id": "COG1307", "cog_category": "I", "go_ids": [ "GO:0008289" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47568", "UniRef:UniRef50_P75312", "COG:COG1307", "COG:I", "GO:0008289" ] }, "pscc": { "uniref50_id": "UniRef50_P75312", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75312" ], "product": "DegV domain-containing protein MG326 homolog" } }, "id": "JPCNHPFPBM_746", "locus": "GCA000292505_00742" }, { "type": "cds", "sequence": "contig_1", "start": 409578, "stop": 410384, "strand": "-", "frame": 2, "gene": "menH", "product": "Putative esterase/lipase 2", "db_xrefs": [ "COG:COG0596", "COG:HR", "EC:3.1.-.-", "GO:0052689", "RefSeq:WP_010869433.1", "SO:0001217", "UniParc:UPI000012A1B5", "UniRef:UniRef100_Q49418", "UniRef:UniRef50_P75311", "UniRef:UniRef90_Q49418" ], "nt": "ATGCTAACGAGTAATAAAAATACTCTTTTTAACTCAATATTTGCCTTTAAACCTAAAAAAAGAAAAAACGTTTTTATTTTTTTACATGGATTTGGAAGCGAATATGCCAGTTTTAGTCGTATTTTTAGCTTGTTTAAAAAGAAAAAGTGGCCTTTCTTTACTTTTAATTTTCCCGGTCATGGAGACAATGAATCAACAGATACAGATCAATTAAAACTAAACCACTTTGTTGATTTGGTTTGTGATTTTATTGTTCAAAAAAAACTAAATAACGTCATTTTAATAGGTCATAGTATGGGAGGTGCTGTGGCTGTTTTAGTTAACAAAGTTATACCACTAAAAATTAAGGCTTTAATATTAGTAGCTCCTATGAATCAAACTTCTTTCTCAGTTAATAAAAAGCGGATTTTAGATACTTTTTTTAAGCGTAATAACAGCAACCATAAGGATTTTGTTGAACATGAAGAAAAACGTAAATCACTTTTGAAAATAGCTATCAATGCATTTAAAAAAAGAACTACCTTTAAAACACTTTACAGTGATATGGTTCAAAACGCTAAATATGGTAATGACTCACTTGAAAGGGCTTATGAAATGATTGGTAATAAACCTACCTTAGTTATTTTAGGAGCTAATGATATTGTCACGCCAACCAAAGCTTCTGTAGACTATCTAGCTAATAAAAGTGACAAGATTATTTTTAAGGTTATTGATGGTGTTGGTCATTCACCACACGACTCAGCGCCTAAACTTTTTTTTGATTATGTGCTAGAGTTTCTAGATAATTTAAAGAAACAAAGGTACTAA", "aa": "MLTSNKNTLFNSIFAFKPKKRKNVFIFLHGFGSEYASFSRIFSLFKKKKWPFFTFNFPGHGDNESTDTDQLKLNHFVDLVCDFIVQKKLNNVILIGHSMGGAVAVLVNKVIPLKIKALILVAPMNQTSFSVNKKRILDTFFKRNNSNHKDFVEHEEKRKSLLKIAINAFKKRTTFKTLYSDMVQNAKYGNDSLERAYEMIGNKPTLVILGANDIVTPTKASVDYLANKSDKIIFKVIDGVGHSPHDSAPKLFFDYVLEFLDNLKKQRY", "aa_hexdigest": "3bffeff7e1f08834f23bc25cb54b35ef", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012A1B5", "ncbi_nrp_id": "WP_010869433.1", "uniref100_id": "UniRef100_Q49418", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012A1B5", "RefSeq:WP_010869433.1", "UniRef:UniRef100_Q49418" ] }, "ips": { "uniref100_id": "UniRef100_Q49418", "uniref90_id": "UniRef90_Q49418" }, "psc": { "uniref90_id": "UniRef90_Q49418", "gene": "MenH", "product": "Putative esterase/lipase 2", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P75311", "cog_id": "COG0596", "cog_category": "HR", "go_ids": [ "GO:0052689" ] }, "pscc": { "uniref50_id": "UniRef50_P75311", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75311" ], "product": "Putative esterase/lipase 2" }, "genes": [ "menH" ], "id": "JPCNHPFPBM_747", "locus": "GCA000292505_00743" }, { "type": "cds", "sequence": "contig_1", "start": 410371, "stop": 410841, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGATGCTAATGAACAATACCAAGATTTACTCTCTGCTTTTGAAGAAACAAACAGTGAATTGGAGAAAGCCAAGCAAAGTTTAAGTGCATCTGATAGTGAAAATAATCAGTTAAAACAACAAATAAATTCACTTGAAAATGCTAAAAAAGAACTTCAATTTACTCCAGTTACCAGTGATGAACATCTTGATGAACTAGAAACATTAAAAATTGAAAAAGAACAACTTTTTTTAGAAAACCAAGCACTGCAAAATCAGTTGCAATATTTCAATGACATTAGTGCTAATCAAACTGAAGAGATCAAAGAAGCTAGTGATGAAGATAAACCAGTTGAAATAAAAAAACCACGAATTAAAAAACGTGATTTTGTTATCCAAAATAAGGATGATAAACTCGCTAAACTTTCTAAGAAAGAACGCATCCAAGCATATGCAGAACGTTTAGCTAAAATCAATGCTAACGAGTAA", "aa": "MNDANEQYQDLLSAFEETNSELEKAKQSLSASDSENNQLKQQINSLENAKKELQFTPVTSDEHLDELETLKIEKEQLFLENQALQNQLQYFNDISANQTEEIKEASDEDKPVEIKKPRIKKRDFVIQNKDDKLAKLSKKERIQAYAERLAKINANE", "aa_hexdigest": "97b4ac2e7bea49e75e88f8111ed3bb99", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18115.818199999998, "isoelectric_point": 4.790356254577637 }, "id": "JPCNHPFPBM_748", "locus": "GCA000292505_00744" }, { "type": "cds", "sequence": "contig_1", "start": 411256, "stop": 412641, "strand": "-", "frame": 1, "gene": "yhgE", "product": "putative protein MG328", "db_xrefs": [ "COG:COG1511", "COG:S", "SO:0001217", "UniRef:UniRef50_Q49419", "UniRef:UniRef90_Q49419" ], "nt": "ATGGCAGTAGATAAGGAACTTGAAGTTAGTGATTTTGATAATGAGCTTGATGAAAAAACACTTTTAAAAGAATTAGTACAAAGAACTAATAATATCCTTTTTTCACCATCTAAAATTACTGCTATTCCCTTTGAAAGAAATTTATTGGAGAAAACATTTTTTGGTACTGTTGATGAAGCTGAGAAAGAAAAGTCAATAGTTTCTTTTTTTAATTGGATGATAGATTTAAAGGTTTTAGATAAAAAATGGGATAAGAATGTTTTAAATCATCATGCAAATCAACTTAAAACTAGAGAGGAAGAGCAACAGACAGTTGATCAAACTATGGCTTTTCAAGAAGTTGATGATCAATCAGTTTTAACCAAGGAAATTAAAACTGGTTTTCAGGAACTAAAACCATCAGTGATTACTGCTGAAGATGATAAGGATGAGATAAAACCAGAAGCTACTAAACAAGTATCTTTTGAAGAGTTGTTTAACCAACCTAGTGAAGAGATTAATGAAACAAAAAAACCAGAAGTTCAAATATTTTCAACTGATAAGGTTAAAGAACCTGAACAATTTGATGATTTCTATTCCATTGAAAATTTAACCAAAGCTATCAATCCAGTTCATAAAACGATCCAATATGATCAGAATGATGATCAACCTTTTGTTGTTAAAAGGATCTTAAAAGAGCAACATCCAACAAAAAAAGTTGATGAATTGGATGACTATAATAACAAAGAGCTTTTATTGGAAAATGCTGATTTAAAAAAACAAATCGATGATCTTAAAGAGAATAATAATGATCAAATATTTGATTTAGAACAGGAAATCGATGATCTTAAAAGGAGGTTATCTGAGGAAAAAAGTAAGCACTTACATACCAAGAAATTACAGGATGATCTTTTGCAAGAAAATAGGGATTTATATGAGCAACTGCAAAATAAACCAGTTGCTATTAATCCTTTAAGTGATGAAGTTAATGAAGAGTTGGAGAATTTAAAACAAGAAAAAGCTTTGCTTTCAGATCAATTAGATGCATTGAAAAATAAGAGCAGTAATGTTCAACAACAATTAGCTTTATTACCAGTTTTAAACAACCAAATAAACGAACTTCAAAACCAGTTACTAACAGCAAGAGAAGCAAACCAAAGATTGGATCTAGTTGAACAAGAAAATGATTTTTTGAAAAATGAACTGAAAAAACTCCATGACAACACATCTAATGATGAGAATGAAAAATATGATGATCTTTTAAATCAGTATGAACTACTTTTTGATGAGAATGAAACAAAATTTGATAAATTGCAAGTGAAACAACAAGCGCTAAACTTAGATTATCAAAAAACAATTTCAGCACTTAAACATGAAAATGATGTTTTATTAGATGAAATTGAGTGA", "aa": "MAVDKELEVSDFDNELDEKTLLKELVQRTNNILFSPSKITAIPFERNLLEKTFFGTVDEAEKEKSIVSFFNWMIDLKVLDKKWDKNVLNHHANQLKTREEEQQTVDQTMAFQEVDDQSVLTKEIKTGFQELKPSVITAEDDKDEIKPEATKQVSFEELFNQPSEEINETKKPEVQIFSTDKVKEPEQFDDFYSIENLTKAINPVHKTIQYDQNDDQPFVVKRILKEQHPTKKVDELDDYNNKELLLENADLKKQIDDLKENNNDQIFDLEQEIDDLKRRLSEEKSKHLHTKKLQDDLLQENRDLYEQLQNKPVAINPLSDEVNEELENLKQEKALLSDQLDALKNKSSNVQQQLALLPVLNNQINELQNQLLTAREANQRLDLVEQENDFLKNELKKLHDNTSNDENEKYDDLLNQYELLFDENETKFDKLQVKQQALNLDYQKTISALKHENDVLLDEIE", "aa_hexdigest": "0e2421413e313b62f8f70d5de25b2370", "start_type": "ATG", "rbs_motif": null, "genes": [ "yhgE" ], "truncated": "5-prime", "pseudogene": { "start": 410953, "stop": 412641, "observations": { "mutation": [ 411258 ], "stop": [ 411258 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49419", "query_cov": 1.0, "subject_cov": 0.6097883597883598, "identity": 0.993, "score": 811.0, "evalue": 2.31e-290, "gene_start": 1, "gene_end": 461, "reference_start": 1, "reference_end": 461, "reference_sequence": "MAVDKELEISDFDNELDEKTLLKELVQRTNNILFSPSKITAIPFERNLLEKTFFGTVDEAEKEKSIVSFFNWMIDLKVLDKKWDKNVLNHYANQLKTREEEQQTVDQTMAFQEVDDQSVLTKEIKTGFQELKPSVITAEDDKDEIKPEATKQVSFEELFNQPSEEINETKKPEVQIFSTDKVKEPEQFDDFYSIENLTKAINPVHKTIQYDQNDDQPFVVKRILKEQHPTKKVDELDDYNNKELLLENADLKKQIDDLKENNNDQIFDLEQEIDDLKRRLSEEKSKHLHTKKLQDDLLQENRDLYEQLQNKPVAINPLSDEVNEELENLKQEKALLSDQLDALKNKSSNVQQQLALLPVLNNQINELQNQLLTAREANQRLDLVEQENDFLKNELKKLHDNTSNDENEKYDDLLNQYELLFDENETKFDKLQVKQQALNLDYQKTISALKHENDVLLDEIEWTRSKDNDFNNTKNSFEEQKKALDEKLNGLTIQNQQLQDKIAELEEWNEEKSNLNTNQLVNLQQQLKDSQNLFNVAQDKLATLEEVNLALNEKINDLEDELSGSENSNNLLAKLQADHEILQESYGKLKTDFEKLKKNKLNDANEQYQDLLSAFEETNSELEKAKQSLSASDSENNQLKQQINSLENAKKELQFTPVTSDEHLDELETLKIEKEQLFLENQALQNQLQYFNDISANQTEEIKEASDEDKPVEIKKPRIKKRDFVIQNKDDKLAKLSKKERIQAYAERLAKINANE" }, "paralog": false, "identity": 0.994661921708185, "score": 1053.0, "evalue": 0.0, "description": "Internal stop codon at 411258. Nonsense mutation around 411258.", "psc": { "uniref90_id": "UniRef90_Q49419", "gene": "YhgE", "product": "Uncharacterized protein MG328", "uniref50_id": "UniRef50_Q49419", "cog_id": "COG1511", "cog_category": "S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49419", "UniRef:UniRef50_Q49419", "COG:COG1511", "COG:S" ] }, "pscc": { "uniref50_id": "UniRef50_Q49419", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49419" ], "product": "Uncharacterized protein MG328" } }, "id": "JPCNHPFPBM_749", "locus": "GCA000292505_00745" }, { "type": "cds", "sequence": "contig_1", "start": 412680, "stop": 414026, "strand": "-", "frame": 2, "gene": "der", "product": "ribosome biogenesis GTPase Der", "db_xrefs": [ "COG:COG1160", "COG:J", "EC:3.6.5.-", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022", "KEGG:K03977", "RefSeq:WP_014894669.1", "SO:0001217", "UniParc:UPI00027B3D31", "UniRef:UniRef100_UPI00027B3D31", "UniRef:UniRef50_P75309", "UniRef:UniRef90_P47571" ], "nt": "GTGTTTACTGTTGCAATAATAGGTCGTACAAACGTTGGTAAATCAACTTTATTTAATCGCTTAATTCAAAAGCCAATGGCTATTGTTTCAGATACTCCTAACACAACAAGAGATAGGATCTTTGGAATTGGTGAATGGTTAAAAAGAAAGATAGCTTTTATTGACACAGGTGGATTAATTGCAAAACAAACTCCATTACAACAACTAATTGCATTACAAGCACAAGCAGCACTTAGTCAAGCCAAAGCTATTATTTTTTTAGTGTCACTACAAGAACAACTAAATAGTGATGATTTTTATGTAGCTAAGGTTCTTAAAAAAAATAAGGATAAACCAGTAATACTAGTGGTAAATAAAGCTGAAAATTTTAACCCTAAAACTGCTGAAGAAACATTAAAAGATTATTACAGCTTAGGGTTTGGAAGACCAGTTGTTATTAGTGCTGCTCATGGAATTGGAATTGGTGATTTAATGGATCTCTTAGTTAAACAAAATCAATTACTCCCTAATGAAAATAATGATGATTTAGTAAAAATAAGGTTTTGTGTAATTGGCAAACCAAATGTTGGTAAAAGCTCACTTATTAATCAACTTGTAAAACAAAATCGTGTTTTAGTTTCAAATGAGAGTGGTACAACTAGAGATGCTATTGATGTTCCTTTAAAAGTTAATGGTGAAAAATTTCTTTTGATTGATACTGCTGGTATTAAAAGAAAAGGCAAAATTAACATGGGAATTGAAACCGCATCTTACATCAAAACAAAGCTAGCTATTGCCCGTAGTAACGTTATCCTTTTGATGGTAGATGGATCAAAACCTATTAGTGAACAAGATGAAGTGATTGGTGGATTGGCACAAGCTGCACTTATCCCTGTTATTATTCTTGTGAATAAATGGGATCTAGTTTTAAAAAACAATAACACCACAAATGCATATAAAAAAATGCTGAAATTGCATTTTAAACACCTCGATTTTGCGCCTGTTTTGTTTATTAGTGTTTTGAAAAATCAGCGCTTAAATACTATTTTTGAACAACTTAAAATTATTCAAAGCCAACTTGAAACTAAGGTTGCAACACCACTTTTAAATGATGTTATTCAGCAAGCACAACTTTACAATCAACCTCCACTTTTTAAAGGCAAGCGTTTGCAGATAACTTACGCAGTACAAACCAAAAGTCAAATTCCCCATTTTGTTTTATTTTGCAATGACCCTAAGTATCTTCATTTTTCTTATGCACGTTTTTTAGAGAATAAGATTAGAGAAAACTTTGGTTTTAATTCTGTACCTATTAGCCTTTACTTTAAATCAAAAAACGCACGGATAAGAACAAAGCCAGAAGTTTAA", "aa": "MFTVAIIGRTNVGKSTLFNRLIQKPMAIVSDTPNTTRDRIFGIGEWLKRKIAFIDTGGLIAKQTPLQQLIALQAQAALSQAKAIIFLVSLQEQLNSDDFYVAKVLKKNKDKPVILVVNKAENFNPKTAEETLKDYYSLGFGRPVVISAAHGIGIGDLMDLLVKQNQLLPNENNDDLVKIRFCVIGKPNVGKSSLINQLVKQNRVLVSNESGTTRDAIDVPLKVNGEKFLLIDTAGIKRKGKINMGIETASYIKTKLAIARSNVILLMVDGSKPISEQDEVIGGLAQAALIPVIILVNKWDLVLKNNNTTNAYKKMLKLHFKHLDFAPVLFISVLKNQRLNTIFEQLKIIQSQLETKVATPLLNDVIQQAQLYNQPPLFKGKRLQITYAVQTKSQIPHFVLFCNDPKYLHFSYARFLENKIRENFGFNSVPISLYFKSKNARIRTKPEV", "aa_hexdigest": "9816d020889f0b4765335aba4bb8a24b", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3D31", "ncbi_nrp_id": "WP_014894669.1", "uniref100_id": "UniRef100_UPI00027B3D31", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3D31", "RefSeq:WP_014894669.1", "UniRef:UniRef100_UPI00027B3D31" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3D31", "uniref90_id": "UniRef90_P47571" }, "psc": { "uniref90_id": "UniRef90_P47571", "gene": "der", "product": "ribosome biogenesis GTPase Der", "ec_ids": [ "3.6.5.-" ], "uniref50_id": "UniRef50_P75309", "cog_id": "COG1160", "cog_category": "J", "kegg_orthology_id": "K03977", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_P75309", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75309" ], "product": "GTPase Der" }, "genes": [ "der" ], "id": "JPCNHPFPBM_750", "locus": "GCA000292505_00746" }, { "type": "cds", "sequence": "contig_1", "start": 414030, "stop": 414476, "strand": "-", "frame": 2, "gene": "cmk", "product": "(d)CMP kinase", "db_xrefs": [ "COG:COG0283", "COG:F", "EC:2.7.4.25", "GO:0004127", "GO:0005524", "GO:0005829", "GO:0006220", "GO:0015949", "GO:0036430", "GO:0036431", "SO:0001217", "UniRef:UniRef50_P47572", "UniRef:UniRef90_P47572" ], "nt": "GTGTATTATAACAATGCTGATATTACAACAGTTATTACAACCCAATCAGTTGCTAACATTGCTAGTAAAATAGCTGTTGATCCTAACATTAGAAAAATTGCAGTTATTAAACAACAGAAACTAGCAGAAAATAAAAACATAGTGATGGATGGTAGAGACATAGGAACAGTAGTTTTAAAAAATGCTCAATTGAAGTTTTTTTTAGATGCTAAAGTTGAAATTAGAGCGCAGCGAAGATTACAAGATATGGGAATTTCTCTATCAAATGAAAAAAAACTAAAGGAACTAATTCAAGAATTAAAGCAACGTGATCAAATTGATAGTTCTAGAACTGCAGACCCATTAAAAAAAGCCCAGGACGCTATTTATCTTGACACTTCTGAACTAAGTTTTGATGCAGTAGTAAAACAAACCCTCAAAGAAGCAAAGAAGGTTTTTAAACTTTAA", "aa": "MYYNNADITTVITTQSVANIASKIAVDPNIRKIAVIKQQKLAENKNIVMDGRDIGTVVLKNAQLKFFLDAKVEIRAQRRLQDMGISLSNEKKLKELIQELKQRDQIDSSRTADPLKKAQDAIYLDTSELSFDAVVKQTLKEAKKVFKL", "aa_hexdigest": "d73d108ff95f14321377c73a3df6cde7", "start_type": "GTG", "rbs_motif": null, "genes": [ "cmk" ], "truncated": "3-prime", "pseudogene": { "start": 414030, "stop": 414683, "observations": { "mutation": [ 414497, 414677 ], "stop": [ 414497, 414677 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47572", "query_cov": 1.0, "subject_cov": 0.6820276497695853, "identity": 0.993, "score": 269.0, "evalue": 7.1e-90, "gene_start": 1, "gene_end": 148, "reference_start": 70, "reference_end": 217, "reference_sequence": "MNWQIAIDGPSSSGKSSVAKKIAEELDFFYFSSGKMYRAFAYVMQVNRLNIDLFLKIINQINWRFEKDAVYYNNADITTVITTQSVANIASKIAVDPNIRKIAVIKQQKLAENKNIVMDGRDIGTVVLKNAQLKFFLDAKVEIRAQRRLQDMGISLSNEKKLKELIQELKQRDQIDSSRTADPLKKAQDAIYLDTSELSFDAVVKQTLKEAKKVFKL" }, "paralog": false, "identity": 0.9907834101382489, "score": 405.0, "evalue": 3.89e-146, "description": "Internal stop codon at 414497, 414677. Nonsense mutation around 414497, 414677.", "psc": { "uniref90_id": "UniRef90_P47572", "gene": "cmk", "product": "(d)CMP kinase", "ec_ids": [ "2.7.4.25" ], "uniref50_id": "UniRef50_P47572", "cog_id": "COG0283", "cog_category": "F", "go_ids": [ "GO:0004127", "GO:0005524", "GO:0005829", "GO:0006220", "GO:0015949", "GO:0036430", "GO:0036431" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47572", "EC:2.7.4.25", "UniRef:UniRef50_P47572", "COG:COG0283", "COG:F", "GO:0004127", "GO:0005524", "GO:0005829", "GO:0006220", "GO:0015949", "GO:0036430", "GO:0036431" ] }, "pscc": { "uniref50_id": "UniRef50_P47572", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47572" ], "product": "Cytidylate kinase" } }, "id": "JPCNHPFPBM_751", "locus": "GCA000292505_00747" }, { "type": "cds", "sequence": "contig_1", "start": 414741, "stop": 414950, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557EBC", "UniRef:UniRef90_UPI0000557EBC" ], "nt": "ATGCTAAACAGCATGAAACTTATTAAAGTTATCACTAAGTTCTTAAAAATAGCCTTATTAGCATTAAACAAATTAGGTTCGATGTTATCAATGCTTTTTTTAAAAGCAAAACTTTCAGTTGAAAAAACTGTTAGTATCTTGTTGTATTCTTTTAAATCATTAGTTAAATTGCTTGTTTTTTTTAATCATTGTTTCTCATTAGCTTCATAA", "aa": "MLNSMKLIKVITKFLKIALLALNKLGSMLSMLFLKAKLSVEKTVSILLYSFKSLVKLLVFFNHCFSLAS", "aa_hexdigest": "d66a2511a7f38532eb179d83cb5663cc", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0000557EBC", "query_cov": 0.8260869565217391, "subject_cov": 1.0, "identity": 1.0, "score": 95.1, "evalue": 1.43e-24, "valid": true, "uniref50_id": "UniRef50_UPI0000557EBC" }, "pscc": { "uniref50_id": "UniRef50_UPI0000557EBC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0000557EBC" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7781.673299999997, "isoelectric_point": 10.252229881286617 }, "id": "JPCNHPFPBM_752", "locus": "GCA000292505_00748" }, { "type": "cds", "sequence": "contig_1", "start": 414998, "stop": 415195, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B4188E", "UniRef:UniRef90_UPI0001B4188E" ], "nt": "TTGATTAATTACCATCCTTTTAGTGAATTTAATAAAAATCCAGTTAAACATCACACTGAACCAAATAAACTGTTTAAAACACTTCAGGAATTAATAGTTGATTTAAAAAATACTGACTTTAAATTATTAGAAGAAAAAGTTGATAGGATGTGGTTAAACGCAGCATATAACCAAACTTCTAGCGGTTATGAAAGCTGA", "aa": "MINYHPFSEFNKNPVKHHTEPNKLFKTLQELIVDLKNTDFKLLEEKVDRMWLNAAYNQTSSGYES", "aa_hexdigest": "1918e9d197e5c33827961012463bbd57", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 414900, "stop": 415330, "observations": { "deletion": [ 414928 ], "mutation": [ 415000, 415231 ], "stop": [ 415000, 415231 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001B4188E", "query_cov": 1.0, "subject_cov": 0.45454545454545453, "identity": 0.985, "score": 135.0, "evalue": 2e-39, "gene_start": 1, "gene_end": 65, "reference_start": 46, "reference_end": 110, "reference_sequence": "MGRVEKFRFYRQSFDNNKIVKKALINAQKNTESWKKQLNKINQKILINYHPFSEFNKNPVKHHTEPNKLFKTLQELIVDLKNTDFKLLEEKVDRMWLNAAYNQTSSGYESWISDDKGIEKINHLSKFYEANEKQWLKKNKQFN" }, "paralog": false, "identity": 0.972027972027972, "score": 275.0, "evalue": 2.42e-97, "description": "Internal stop codon at 415000, 415231. Frameshift due to deletion around 414928. Nonsense mutation around 415000, 415231.", "psc": { "uniref90_id": "UniRef90_UPI0001B4188E", "uniref50_id": "UniRef50_UPI0001B4188E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001B4188E", "UniRef:UniRef50_UPI0001B4188E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0001B4188E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0001B4188E" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7743.653400000003, "isoelectric_point": 6.254642295837402 }, "id": "JPCNHPFPBM_753", "locus": "GCA000292505_00749" }, { "type": "cds", "sequence": "contig_1", "start": 415229, "stop": 415330, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAGAGTAGAAAAATTTCGCTTTTATAGACAAAGTTTTGATAACAATAAGATAGTTAAAAAAGCTTTAATTAACGCTCAGAAGAACACTGAAAGCTGA", "aa": "MGRVEKFRFYRQSFDNNKIVKKALINAQKNTES", "aa_hexdigest": "218d173b86910ef8438da546ab36ae66", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3961.5091, "isoelectric_point": 10.282916831970212 }, "id": "JPCNHPFPBM_754", "locus": "GCA000292505_00750" }, { "type": "cds", "sequence": "contig_1", "start": 415318, "stop": 416037, "strand": "-", "frame": 1, "gene": "tACO1", "product": "putative transcriptional regulatory protein MG332", "db_xrefs": [ "COG:COG0217", "COG:KJ", "GO:0003677", "GO:0005829", "GO:0006355", "RefSeq:WP_009885985.1", "SO:0001217", "UniParc:UPI000013963B", "UniRef:UniRef100_P47574", "UniRef:UniRef50_P47574", "UniRef:UniRef90_P47574" ], "nt": "ATGCCACGTAAGCATCTAATTGCTAATCAAACTAATAAAAAACAACAAACAAGTGCAAAACAACTTCAAAAACTAGCAAAAAGAATAGCTTCAGCTGTTAAAAAAGGTGGAACTAATATCCAGTCAAATCCACATCTAAAAGTTGCAGTTGATCTTGCTTTAGCTAAGGGTCTAAGCATGGATTCAATTAAAAGAAATATCCATGGTAGTGAAAAAGATACAACTAAAATTAGCGAGTTTTGTTATGAGATTTTTGGACCAAATGGTGTTGGAATTATTGTGTTTGGACTAACTGATAATCCTAACCGTTTACTCAGTAGTTTAAACGGTTATTTAGCTAAACTAAAAGGACAATTAGCCAAGCCAAATAGCGTCAAGATTAATTTTCAAGAAGAAGGGATTATCTTTGTTAATAAAAATAACTATTTGAAAGATGATCTAATTGAATTATTAATTTTGGACAATATTAACTTAATTGATGTTGATTATGATGAAGAGTGTTTTGAAATTAGCTTGCATTCAAATAGTTATTTTCATGCAAAGGAACTGTTGAAAAAAAACAATTTTTCAATAGTAGATAGTGAAATTAAATTGGTACCTCTTTTAACTGTTGATTTAGATAGAAATCAGCAAACTTTATTATCACGTTTTCTCAATGCTTGTGAGGAAGATGATGACATTCAGTTTGTTGTTCATAATGCCAACCCATGGGAAGAGTAG", "aa": "MPRKHLIANQTNKKQQTSAKQLQKLAKRIASAVKKGGTNIQSNPHLKVAVDLALAKGLSMDSIKRNIHGSEKDTTKISEFCYEIFGPNGVGIIVFGLTDNPNRLLSSLNGYLAKLKGQLAKPNSVKINFQEEGIIFVNKNNYLKDDLIELLILDNINLIDVDYDEECFEISLHSNSYFHAKELLKKNNFSIVDSEIKLVPLLTVDLDRNQQTLLSRFLNACEEDDDIQFVVHNANPWEE", "aa_hexdigest": "8eb09ed5c376415173eb966cf3d8aec2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013963B", "ncbi_nrp_id": "WP_009885985.1", "uniref100_id": "UniRef100_P47574", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013963B", "RefSeq:WP_009885985.1", "UniRef:UniRef100_P47574" ] }, "ips": { "uniref100_id": "UniRef100_P47574", "uniref90_id": "UniRef90_P47574" }, "psc": { "uniref90_id": "UniRef90_P47574", "gene": "TACO1", "product": "Probable transcriptional regulatory protein MG332", "uniref50_id": "UniRef50_P47574", "cog_id": "COG0217", "cog_category": "KJ", "go_ids": [ "GO:0005829", "GO:0003677", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_P47574", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47574" ], "product": "Probable transcriptional regulatory protein MG332" }, "genes": [ "tACO1" ], "id": "JPCNHPFPBM_755", "locus": "GCA000292505_00751" }, { "type": "cds", "sequence": "contig_1", "start": 416044, "stop": 416181, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGGTTTTGCAGGTGTTACACATTTTGAGAGTGTTTTAATTGATGACACTAAAATTCTTTACAAAGATAAAAACAAGCAAGAGGTAGTTGATCTGTTTGCACACAAAGTTGACCAAGTTGCTAATAATTTTTAA", "aa": "MFGFAGVTHFESVLIDDTKILYKDKNKQEVVDLFAHKVDQVANNF", "aa_hexdigest": "82e4ebb11438c9b9f04963f8376f769a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5172.820200000002, "isoelectric_point": 5.393304252624512 }, "id": "JPCNHPFPBM_756", "locus": "GCA000292505_00752" }, { "type": "cds", "sequence": "contig_1", "start": 416218, "stop": 416382, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02525.22" ], "nt": "ATGACCAACTTTAATTATCCTGCTAGTTTAAAAAACTTTATTGATCATGTCTTTGTAGCTAATAAAACCTTTCAAGACAAATATGTTACTAAAGGTGCTTCTAAGGGATTACTTACTAATCTTAAGGTAGTTGTTTTAGCGTCACAAGGTGCACCATTAGGATGA", "aa": "MTNFNYPASLKNFIDHVFVANKTFQDKYVTKGASKGLLTNLKVVVLASQGAPLG", "aa_hexdigest": "cd4115273f6ad6fa7b62f103ec3e3d2f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02525.22", "name": "Flavodoxin_2", "length": 52, "aa_cov": 0.9629629629629629, "hmm_cov": 0.9807692307692307, "evalue": 3.3604035364541894e-13, "score": 44.84715270996094, "start": 1, "stop": 52 } ], "seq_stats": { "molecular_weight": 5857.734000000002, "isoelectric_point": 9.778580665588379 }, "id": "JPCNHPFPBM_757", "locus": "GCA000292505_00753" }, { "type": "cds", "sequence": "contig_1", "start": 416515, "stop": 416637, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAGTTATTATTGTTGATGCTTCTGTAACCCCTTCTGGTTCTTACACCCACCTTTTATTAGATCGTTTCCTTCAAACTTATAAAAAACAAAATAGTAGTGTTGAATTTATTGATTGA", "aa": "MKKVIIVDASVTPSGSYTHLLLDRFLQTYKKQNSSVEFID", "aa_hexdigest": "8f3084ab4a5b37a563b88e61619cbfe8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4573.226500000001, "isoelectric_point": 8.12071170806885 }, "id": "JPCNHPFPBM_758", "locus": "GCA000292505_00754" }, { "type": "cds", "sequence": "contig_1", "start": 417502, "stop": 417735, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGTTATTTAACTCCTTTTTTGAAACTAAAAAACTGCCATTTAAAACTGTTTTAATCCACGGTTTAGTACGCGATGAACAAAATCGTAAGATGTCAAAATCACTGAATAATGGCATTGATCCTGTTGATCTTATTAGAAATTATGGAGCGGATGCAGTGCGCTTATTTTTGTGTTCAAATCACACTCCAGGAGATGATCTAATTTTCAGCGAACAAAAAATAAAAAGTGCATGA", "aa": "MLFNSFFETKKLPFKTVLIHGLVRDEQNRKMSKSLNNGIDPVDLIRNYGADAVRLFLCSNHTPGDDLIFSEQKIKSA", "aa_hexdigest": "4ca030f6146eb7eee758fc78ef2bb7eb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001D01CCB6", "query_cov": 0.974025974025974, "subject_cov": 0.8620689655172413, "identity": 0.547, "score": 90.1, "evalue": 4.21e-22, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A3B0PEY5" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 61, "aa_cov": 0.7922077922077922, "hmm_cov": 0.9836065573770492, "evalue": 3.3357487459360438e-21, "score": 70.54769897460938, "start": 7, "stop": 67 } ], "seq_stats": { "molecular_weight": 8784.024499999996, "isoelectric_point": 8.889432716369633 }, "id": "JPCNHPFPBM_759", "locus": "GCA000292505_00755" }, { "type": "cds", "sequence": "contig_1", "start": 418207, "stop": 418368, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATGTACTCCTGCACACGACTTTAATGACTATGAAATCAACACTAAATATAAATTTGATTTTCTAAGCTGCATTGACAGTAACGGTATTCTCAATCAAAATGCAAGTAAATTTCAAGGCCTTAGTGTTTTACAAGCAAGAAATAAAATTGTTAAATGA", "aa": "MKCTPAHDFNDYEINTKYKFDFLSCIDSNGILNQNASKFQGLSVLQARNKIVK", "aa_hexdigest": "c4bd564551a58b5abec6317514ccb895", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6069.855600000002, "isoelectric_point": 8.685003471374515 }, "id": "JPCNHPFPBM_760", "locus": "GCA000292505_00756" }, { "type": "cds", "sequence": "contig_1", "start": 418456, "stop": 418644, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATACTGCTATATCAAATATTGAAGTTATCAATAAACCTGTTAATCAACATCTTCATTATGTTGTTTATAAACTAGCGAATGATAGTAAACAAGAACTAATAGTTGCAACAACAAGACCAGAAACTATCTTTGCTGATGTTTGTCTATTGGTAAACCCAAAAGATAAGCGCTATACTAATTTCTGA", "aa": "MNTAISNIEVINKPVNQHLHYVVYKLANDSKQELIVATTRPETIFADVCLLVNPKDKRYTNF", "aa_hexdigest": "6d270352913e18d0c0d424f6414d3ca7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7132.138000000002, "isoelectric_point": 7.9005521774292005 }, "id": "JPCNHPFPBM_761", "locus": "GCA000292505_00757" }, { "type": "cds", "sequence": "contig_1", "start": 419177, "stop": 419752, "strand": "-", "frame": 3, "gene": "yihA", "product": "ribosome biogenesis GTP-binding protein YihA/YsxC", "db_xrefs": [ "COG:COG0218", "COG:D", "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0042254", "GO:0043022", "GO:0046872", "RefSeq:WP_014894297.1", "SO:0001217", "UniParc:UPI00027B3D34", "UniRef:UniRef100_UPI00027B3D34", "UniRef:UniRef50_P47577", "UniRef:UniRef90_P47577" ], "nt": "ATGGATGCACATTTTTTGAAGAGTGCAAGTGATTTAAAAGATTGTCCTCAAGATAATATCCCAGAGATTTGTTTCATGGGAAGAAGTAATGTTGGTAAATCAAGTTTGATTAATGCTTTTTTTAAAAAGAAACTGGCTAAAACTTCAGCAACACCAGGTCGAACACAATTACTAAATTACTTTGAATATAAAGACAAAAGATTTGTTGATTTACCAGGTTATGGCTTTGCTAAAATAAGCAAAAATAAGAAAGATTTTATTACTAATTTACTTACCCAATTTTTAAATTTTCGTAGTAATCTAGTTGGTGTTGTTTTGATAGTAGATAGTGGAGTTGTTACTGTTCAAGATCAGGAAGTGGTTAAAATTATTTTGCAAACAGGATTGAACTTTTTGATTGTTGCTAATAAATTTGACAAACTAAATCAAAGTGAAAGATATTTTTCGCTTAAGAATATTGCAAATTTTTTAAAAGTTAATTTTGATAAATGTGTTTTTGCAAGTACAAAAACACATCATAATCTTGCTTTAGTTCACAAAAAAATTTTCGAATTATTCGTTGAAGATGAAAGATAA", "aa": "MDAHFLKSASDLKDCPQDNIPEICFMGRSNVGKSSLINAFFKKKLAKTSATPGRTQLLNYFEYKDKRFVDLPGYGFAKISKNKKDFITNLLTQFLNFRSNLVGVVLIVDSGVVTVQDQEVVKIILQTGLNFLIVANKFDKLNQSERYFSLKNIANFLKVNFDKCVFASTKTHHNLALVHKKIFELFVEDER", "aa_hexdigest": "b038ecfc1be45f7b55bbe3ccc377b14d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3D34", "ncbi_nrp_id": "WP_014894297.1", "uniref100_id": "UniRef100_UPI00027B3D34", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3D34", "RefSeq:WP_014894297.1", "UniRef:UniRef100_UPI00027B3D34" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3D34", "uniref90_id": "UniRef90_P47577" }, "psc": { "uniref90_id": "UniRef90_P47577", "gene": "yihA", "product": "ribosome biogenesis GTP-binding protein YihA/YsxC", "uniref50_id": "UniRef50_P47577", "cog_id": "COG0218", "cog_category": "D", "go_ids": [ "GO:0000917", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0005829", "GO:0042254", "GO:0043022", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47577", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47577" ], "product": "Probable GTP-binding protein EngB" }, "genes": [ "yihA" ], "id": "JPCNHPFPBM_762", "locus": "GCA000292505_00758" }, { "type": "cds", "sequence": "contig_1", "start": 419760, "stop": 419981, "strand": "-", "frame": 2, "gene": "yneF", "product": "UPF0154 MG335-1-like protein", "db_xrefs": [ "COG:COG3763", "COG:S", "GO:0016020", "KEGG:K09976", "RefSeq:WP_009885966.1", "SO:0001217", "UniParc:UPI0000139652", "UniRef:UniRef100_Q49310", "UniRef:UniRef50_Q9EXD7", "UniRef:UniRef90_Q9EXD7" ], "nt": "ATGAATGATCTAGCACTTGCATTAGGTTTAGGTATCCCTCTTTCGCTTTTGGTGGGAATGATTCTTGGTTATTTTATTTCTATAAAAATCTTTAAAAAACAGATGCGTGACAATCCACCTATTACTGAAAATCAAATTAAAGCAATGTATGCAAAAATGGGTAGAAAATTATCTGAAACCCAGGTAAAAGAGATAATGCGCTCCATTAAAAACCAAAAATAA", "aa": "MNDLALALGLGIPLSLLVGMILGYFISIKIFKKQMRDNPPITENQIKAMYAKMGRKLSETQVKEIMRSIKNQK", "aa_hexdigest": "4ea5b5ae143c256ba0c296af9bf55d2d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139652", "ncbi_nrp_id": "WP_009885966.1", "uniref100_id": "UniRef100_Q49310", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139652", "RefSeq:WP_009885966.1", "UniRef:UniRef100_Q49310" ] }, "ips": { "uniref100_id": "UniRef100_Q49310", "uniref90_id": "UniRef90_Q9EXD7" }, "psc": { "uniref90_id": "UniRef90_Q9EXD7", "gene": "YneF", "product": "UPF0154 protein MG335.1 homolog", "uniref50_id": "UniRef50_Q9EXD7", "cog_id": "COG3763", "cog_category": "S", "kegg_orthology_id": "K09976", "go_ids": [ "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_Q9EXD7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9EXD7" ], "product": "UPF0154 protein MG335.1 homolog" }, "genes": [ "yneF" ], "id": "JPCNHPFPBM_763", "locus": "GCA000292505_00759" }, { "type": "cds", "sequence": "contig_1", "start": 420040, "stop": 420552, "strand": "+", "frame": 1, "gene": "wcaA", "product": "Processive diacylglycerol beta-glycosyltransferase", "db_xrefs": [ "COG:COG0463", "COG:M", "EC:2.4.1.-", "GO:0005886", "GO:0006071", "GO:0009246", "GO:0016758", "GO:0046467", "GO:0046509", "KEGG:K19004", "SO:0001217", "UniRef:UniRef50_Q9ZB73", "UniRef:UniRef90_Q9ZB73" ], "nt": "ATGGATAAACTTGTTAGTATATTAGTTCCTTGTTACAAATCAAAACCTTTTTTAAAACGTTTTTTTAATTCACTTTTAAAGCAAGATCTTAATCAAGCTAAAATTATTTTTTTCAATGACAATGTTGCTGATGAAACCTATGAAGTTTTGCAAAAATTCAAAAAAGAACACAATAACTTAGCAATTGAAGTCTATTGTGACAAACAGAATGAAGGTATTGGAAAAGTGCGTGATAAGCTAGTGAATCTAGTAACAACACCTTATTTTTATTTTATCGATCCTGATGATTGTTTTAACAACAAAAATGTCATAAAAGAGATTGTTGAATCAATTAAAAAAGAAGATTTTGATCTTGGTGTATTAAAAAGTATGGTCTATTTATGCTTCTTAAAACATGATTTCATTATTAAATTTTTGCCTTTAAAAGGTATTTTTCAAGGCAGAGTAAAATTAATTAATAATAACAATGTTAATAAATTAAATTACATCAAAAATAATGATCAATATATTTGA", "aa": "MDKLVSILVPCYKSKPFLKRFFNSLLKQDLNQAKIIFFNDNVADETYEVLQKFKKEHNNLAIEVYCDKQNEGIGKVRDKLVNLVTTPYFYFIDPDDCFNNKNVIKEIVESIKKEDFDLGVLKSMVYLCFLKHDFIIKFLPLKGIFQGRVKLINNNNVNKLNYIKNNDQYI", "aa_hexdigest": "b0c887849268e8f6998972891a0c9469", "start_type": "ATG", "rbs_motif": null, "genes": [ "wcaA" ], "truncated": "3-prime", "pseudogene": { "start": 420040, "stop": 420855, "observations": { "mutation": [ 420550, 420631, 420844 ], "stop": [ 420550, 420631, 420844 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9ZB73", "query_cov": 1.0, "subject_cov": 0.49853372434017595, "identity": 1.0, "score": 345.0, "evalue": 1.22e-117, "gene_start": 1, "gene_end": 170, "reference_start": 1, "reference_end": 170, "reference_sequence": "MDKLVSILVPCYKSKPFLKRFFNSLLKQDLNQAKIIFFNDNVADETYEVLQKFKKEHNNLAIEVYCDKQNEGIGKVRDKLVNLVTTPYFYFIDPDDCFNNKNVIKEIVESIKKEDFDLGVLKSMVYLCFLKHDFIIKFLPLKGIFQGRVKLINNNNVNKLNYIKNNDQYIWNIVINTDFFRKLNLTFESRLFEDIPIWYPMFFSSQKIVFIDVIGTNYFIRNDSLSTTISAPRYLNLIQCYEKLYVNLSQNGSLASFIDPNHKIEARFWRRQMFVWFALFSFEYFKKNFSESKKILEKLFVFLEKNGVYERVFQTKNQGIYYIWVQRLKYFKHVLESKSDN" }, "paralog": false, "identity": 0.988929889298893, "score": 538.0, "evalue": 2.55e-196, "description": "Internal stop codon at 420550, 420631, 420844. Nonsense mutation around 420550, 420631, 420844.", "psc": { "uniref90_id": "UniRef90_Q9ZB73", "gene": "WcaA", "product": "Processive diacylglycerol beta-glycosyltransferase", "ec_ids": [ "2.4.1.-" ], "uniref50_id": "UniRef50_Q9ZB73", "cog_id": "COG0463", "cog_category": "M", "kegg_orthology_id": "K19004", "go_ids": [ "GO:0005886", "GO:0046509", "GO:0016758", "GO:0009246", "GO:0006071", "GO:0046467" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9ZB73", "EC:2.4.1.-", "UniRef:UniRef50_Q9ZB73", "COG:COG0463", "COG:M", "KEGG:K19004", "GO:0005886", "GO:0046509", "GO:0016758", "GO:0009246", "GO:0006071", "GO:0046467" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZB73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZB73" ], "product": "Processive diacylglycerol beta-glycosyltransferase" } }, "id": "JPCNHPFPBM_764", "locus": "GCA000292505_00760" }, { "type": "cds", "sequence": "contig_1", "start": 420640, "stop": 420846, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTTTCATCACAAAAAATTGTTTTTATTGATGTGATAGGAACAAATTATTTTATTCGTAATGATAGTTTATCAACTACTATTAGTGCTCCACGCTACTTAAATTTAATCCAATGTTATGAAAAGCTATATGTAAATCTCAGCCAAAATGGTTCTCTTGCAAGTTTTATTGATCCAAATCATAAGATTGAAGCTAGGTTTTGA", "aa": "MFFSSQKIVFIDVIGTNYFIRNDSLSTTISAPRYLNLIQCYEKLYVNLSQNGSLASFIDPNHKIEARF", "aa_hexdigest": "2301ad5b52bd201c850b9dca25639653", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7853.892699999998, "isoelectric_point": 7.8923646926879885 }, "id": "JPCNHPFPBM_765", "locus": "GCA000292505_00761" }, { "type": "cds", "sequence": "contig_1", "start": 421174, "stop": 421557, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00266.25" ], "nt": "ATGTCTGCAATTAAATTTAATCCTAGTTCATTCAGAAAAAACTTTAAATGGTTTGAAAATAACAAAAATTGGATTAATTTTGATAATGCTGCTACTTCCATTGCACTTGATGTTGTGGCTGAAGCAAGCAAAGAATATTACCAGTATTTTTGTGTCAATCCTCATAACAAAAATCCTGAAATTAACCAAAAACTTATTGCTATTATTGAAGAAACAAGAGATTTATTAGCAAAATTTTTCAATGCTAAAAAAAATGAAATAATTTTTACAAGTTCTGCAACTGAATCGCTTAACTTATTCGCCTTTGGATTAAGCTCTTTAGTAAAAAGTAATGATGAAATCATTCTCAAAGAAGATGAACATGCTGCTAATGTTTTTCCCTGA", "aa": "MSAIKFNPSSFRKNFKWFENNKNWINFDNAATSIALDVVAEASKEYYQYFCVNPHNKNPEINQKLIAIIEETRDLLAKFFNAKKNEIIFTSSATESLNLFAFGLSSLVKSNDEIILKEDEHAANVFP", "aa_hexdigest": "d2254112d2d3c44a17244c1b4c4922ba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00266.25", "name": "Aminotran_5", "length": 104, "aa_cov": 0.8110236220472441, "hmm_cov": 1.0, "evalue": 3.476609800402396e-27, "score": 90.3916015625, "start": 25, "stop": 127 } ], "seq_stats": { "molecular_weight": 14573.285499999982, "isoelectric_point": 5.6869913101196286 }, "id": "JPCNHPFPBM_766", "locus": "GCA000292505_00762" }, { "type": "cds", "sequence": "contig_1", "start": 421945, "stop": 422400, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00266.25" ], "nt": "TTGGATAATAACTTTAAGATAATTTTTAAAGAAGGTCCTTCCAAATTTGAAGCTGGAACGCTAAATATTATGGCTATTTATGCTTTGAATAAACAGTTAAAATTCATGCAAAAAGAATTTAATTTCAGTGAAATGGTGTTTTACAGCAAACAATTAAAAAATTTAGCTTATCAACTGCTAAGTCAAAATCCTAATATCGTTTTAGCTAATCATGATCAAGATGTTCCTATCTTTGCTTTTAAGCATAAATATATTAATTCTGCAGATCTAGCAACTTTTTTAAACATTAAAAAAATAATTGTTAGACAAGGATCCATCTGTGTTGGTAAATTTAAAAATAAAGAGAGTTTTTTACGTGTTTCTCTACTCCATTACAACACAAAAGAGGAATTACTTTATTTAGAAAAATTATTAAAAACTAGTAAGAATTCCATTATTAATGAACTAATATATTAG", "aa": "MDNNFKIIFKEGPSKFEAGTLNIMAIYALNKQLKFMQKEFNFSEMVFYSKQLKNLAYQLLSQNPNIVLANHDQDVPIFAFKHKYINSADLATFLNIKKIIVRQGSICVGKFKNKESFLRVSLLHYNTKEELLYLEKLLKTSKNSIINELIY", "aa_hexdigest": "97b774aef007d732051569d90b9d2d75", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00266.25", "name": "Aminotran_5", "length": 133, "aa_cov": 0.8410596026490066, "hmm_cov": 0.9924812030075187, "evalue": 3.1535770161102735e-30, "score": 100.40299987792969, "start": 7, "stop": 133 } ], "seq_stats": { "molecular_weight": 17636.471600000004, "isoelectric_point": 9.49923915863037 }, "id": "JPCNHPFPBM_767", "locus": "GCA000292505_00763" }, { "type": "cds", "sequence": "contig_1", "start": 422404, "stop": 422820, "strand": "+", "frame": 1, "gene": "iscU", "product": "putative protein MG337", "db_xrefs": [ "COG:COG0822", "COG:O", "GO:0005506", "GO:0016226", "GO:0051536", "RefSeq:WP_009885968.1", "SO:0001217", "UniParc:UPI0000139647", "UniRef:UniRef100_P47579", "UniRef:UniRef50_P47579", "UniRef:UniRef90_P47579" ], "nt": "ATGGATATTAGAGCAAGAACAAAAATTATTGATATTTATTCAAATTTAAAATACAAAAAACCTCTCAAAAGTTTTCAAAAAATTTTAACAACAAGTGATAGTGATAATTGTGAAGACTTTTTTAATATAGGTTTAAATATTGATAAAAATAAAATTACTGCAATTGGTTTTGATGGTGATGGTTGTATTATCTCTACAATTGCAACTGAATTAAGTATTAAAGCAATTGAAAATAAAACTATTAACCAAGCAAAAAAAATATTATCAGATTTAATTGCAACTTATAAAGATAAAAATTCAGCAAACCAAGTTGTTGAAGAATTAAAGCTTTTAATTGAAATGAATGTAACTGAAAAAAGGTTACAGTGTTTGCTTTTAACACCAAGCAACTTATTGCAATGGTTTAAAAATTTTTAG", "aa": "MDIRARTKIIDIYSNLKYKKPLKSFQKILTTSDSDNCEDFFNIGLNIDKNKITAIGFDGDGCIISTIATELSIKAIENKTINQAKKILSDLIATYKDKNSANQVVEELKLLIEMNVTEKRLQCLLLTPSNLLQWFKNF", "aa_hexdigest": "0dc246878c31421d62ae0f5649a611e9", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139647", "ncbi_nrp_id": "WP_009885968.1", "uniref100_id": "UniRef100_P47579", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139647", "RefSeq:WP_009885968.1", "UniRef:UniRef100_P47579" ] }, "ips": { "uniref100_id": "UniRef100_P47579", "uniref90_id": "UniRef90_P47579" }, "psc": { "uniref90_id": "UniRef90_P47579", "gene": "IscU", "product": "Uncharacterized protein MG337", "uniref50_id": "UniRef50_P47579", "cog_id": "COG0822", "cog_category": "O", "go_ids": [ "GO:0005506", "GO:0051536", "GO:0016226" ] }, "pscc": { "uniref50_id": "UniRef50_P47579", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47579" ], "product": "Uncharacterized protein MG337" }, "genes": [ "iscU" ], "id": "JPCNHPFPBM_768", "locus": "GCA000292505_00764" }, { "type": "cds", "sequence": "contig_1", "start": 422807, "stop": 422983, "strand": "-", "frame": 3, "gene": null, "product": "DUF3713 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FDF155B", "UniRef:UniRef90_UPI002FDF155B" ], "nt": "ATGTCTTTACTTATTGAACAAGCTTTAGATGAAGGCACTCAAGAGTTGGCATTTTTAGATTTAATTCGTGAAAATCAAAAGCAACACCAAAGAGAACATCAACAGATTTTAGTTGGTGATAAACGCTTATATGATATCCTAGGACAAACGTTAGCTATTAATGCTAAAAATTTTTAA", "aa": "MSLLIEQALDEGTQELAFLDLIRENQKQHQREHQQILVGDKRLYDILGQTLAINAKNF", "aa_hexdigest": "94939ef73fd11265fe013b81829fdd69", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 422807, "stop": 423241, "observations": { "mutation": [ 423031 ], "stop": [ 423031 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FDF155B", "query_cov": 1.0, "subject_cov": 0.4027777777777778, "identity": 1.0, "score": 111.0, "evalue": 2.89e-30, "gene_start": 1, "gene_end": 58, "reference_start": 87, "reference_end": 144, "reference_sequence": "MLNVVDRLDDLYFHKFEGYVGLSKAANTDGSNTEGYKTVQTVPLTNDSSIQTLSYVIQITNDDVNKISSNWKNNKANSLGLKKEIFMSLLIEQALDEGTQELAFLDLIRENQKQHQREHQQILVGDKRLYDILGQTLAINAKNF" }, "paralog": false, "identity": 0.9861111111111112, "score": 279.0, "evalue": 5.82e-99, "description": "Internal stop codon at 423031. Nonsense mutation around 423031.", "psc": { "uniref90_id": "UniRef90_UPI002FDF155B", "product": "DUF3713 domain-containing protein", "uniref50_id": "UniRef50_UPI002FDF155B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FDF155B", "UniRef:UniRef50_UPI002FDF155B" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FDF155B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FDF155B" ], "product": "DUF3713 domain-containing protein" } }, "id": "JPCNHPFPBM_769", "locus": "GCA000292505_00765" }, { "type": "cds", "sequence": "contig_1", "start": 423029, "stop": 423385, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAATAAAACCAGTTCTGATGCTGAACTTAATGCTTTTGGTGAGTTATTACATCGCTCAGTTAATGTTGATACAAATAATTTAAGTCGCTTTAACTCTAGAGGTGAACCTTTAATAAGTTTTGATAATAAAAAAAAGTTTTTATTAAATGTAGTTGATAGACTAGATGATCTTTATTTTCATAAATTTGAAGGTTATGTTGGTTTATCTAAAGCTGCAAATACAGATGGTTCCAATACAGAAGGATATAAAACTGTGCAAACAGTTCCATTAACAAATGACTCTAGTATCCAGACTCTTAGTTATGTTATTCAGATAACAAATGATGATGTAAATAAGATTTCTAGTAATTGA", "aa": "MINKTSSDAELNAFGELLHRSVNVDTNNLSRFNSRGEPLISFDNKKKFLLNVVDRLDDLYFHKFEGYVGLSKAANTDGSNTEGYKTVQTVPLTNDSSIQTLSYVIQITNDDVNKISSN", "aa_hexdigest": "40d7784c5e3ec3fe7bbebb82ad286c73", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13179.420299999983, "isoelectric_point": 5.2397253036499025 }, "id": "JPCNHPFPBM_770", "locus": "GCA000292505_00766" }, { "type": "cds", "sequence": "contig_1", "start": 423389, "stop": 423646, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGAAAGTGAAAACACTTATAGATTTACTGATGAACCGTTTAATAATTCTGTAGCTTTATCTAATAAATCACAAGGATCAAGTGATAAAAAATATTTTTATGGATTTAATGGTTTAACAATTAATTCCAATCAAAGTATTTCAACAGCTAGTGCTGGTTTAACACAACAGCTTTTTAATAATTACGGACAGTTAATTACAGCAACTGATAAAGCAGGCGCTTTAAGTCAATATAAGGATAAGTTTACCTTGTGA", "aa": "MLESENTYRFTDEPFNNSVALSNKSQGSSDKKYFYGFNGLTINSNQSISTASAGLTQQLFNNYGQLITATDKAGALSQYKDKFTL", "aa_hexdigest": "ac5100230865ba6ce0437b357380d75e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9393.166299999992, "isoelectric_point": 6.079976081848144 }, "id": "JPCNHPFPBM_771", "locus": "GCA000292505_00767" }, { "type": "cds", "sequence": "contig_1", "start": 423899, "stop": 424057, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCAAATGCAAAATAAAGCTGCCCAATCTCATACTTCTAGTAGTGTTAATCAAACCCTATTAGATTTAGCAAAGAAAGCATTTGAAAAGGAATTGGAAGATCCTAATCAGGATGCTGAGTACAAATACATGCGCTTTTTGCAAGCGTTAATGTGA", "aa": "MLQMQNKAAQSHTSSSVNQTLLDLAKKAFEKELEDPNQDAEYKYMRFLQALM", "aa_hexdigest": "76498baee34a784911fae9d47657d9b2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6035.8148, "isoelectric_point": 5.6171361923217775 }, "id": "JPCNHPFPBM_772", "locus": "GCA000292505_00768" }, { "type": "cds", "sequence": "contig_1", "start": 424127, "stop": 424324, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATCTTGATCTTATATTATCACTTTTAACTGATTCAGGAATTAGAACAGGTACTGTTGCAAACACCTTTAAAAATTGGTACTTTAAAAACACTAATTCATTCACTAATAATTTTGATACTTCAAATAAAGAATTAAAAGGTGAATTTTCTGATTTTAATGATCTTGTTAAACAGGCACTTTATATCCGTTCTTGA", "aa": "MDLDLILSLLTDSGIRTGTVANTFKNWYFKNTNSFTNNFDTSNKELKGEFSDFNDLVKQALYIRS", "aa_hexdigest": "d1a61a0853a8d07dc47d810fe88755a3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7474.2420999999995, "isoelectric_point": 5.03322925567627 }, "id": "JPCNHPFPBM_773", "locus": "GCA000292505_00769" }, { "type": "cds", "sequence": "contig_1", "start": 424394, "stop": 424912, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGAAAATGGAACTTCTAATAATAACGGTCAAAAAGATGTATGTGATTTAGCTAAAAAACTTTTAAAAAACAATACCAATTTATCTGAAACTATTAAAAAACAACAAGATTTTAATAATTCACTTTCACAAATAAAATCAAGTTATGAGTCTTATATTAAAGCTGCTAATAAACTTAATAACTTTAGAACAGATTTAGCTAATATAGAAAAACTAGAACAAGCAATTGTTGATCGTGCTAACAACTACATTAAACTTCAAAAGGAAGCTAAAGAAAGTTCAATTGGTTGGGGTCAACCTCTTCCATATAAAAGAGCAAATGATGGTAGTTATCCTTCATTAGCAAAATTTTTTAATAACAACTCTGATCAAAGTAGTGCTCAAACTTTAACTTTGAAAACCACTGCTGCAGCTATTACTTCTGATAATGAACCAACTCGTGAAAAAAATAATCAAACTTTAAAGACTTTAACAACTGAAGTAGAAAATAAAGCCAAAGAATTAGTTGAAAAATGA", "aa": "MLENGTSNNNGQKDVCDLAKKLLKNNTNLSETIKKQQDFNNSLSQIKSSYESYIKAANKLNNFRTDLANIEKLEQAIVDRANNYIKLQKEAKESSIGWGQPLPYKRANDGSYPSLAKFFNNNSDQSSAQTLTLKTTAAAITSDNEPTREKNNQTLKTLTTEVENKAKELVEK", "aa_hexdigest": "8aeecd8dd1ab99f285420dd9af9bdee1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19280.205400000006, "isoelectric_point": 9.025138664245606 }, "id": "JPCNHPFPBM_774", "locus": "GCA000292505_00770" }, { "type": "cds", "sequence": "contig_1", "start": 424958, "stop": 425263, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATCAAATTCATCTCAAGCACAAGTAGTAACAAGTAATTTATTAGTTAGTCTAAGTTCAAAGACAACTCAAAAACAGCAACAGAAACCTGTTTATGTTAGAGGTGATGATGCCATATATGCATTTCATATTGATGGAGGTAATTATTTTTTAGAGAATTCATCACCTAACAAACGTAATTTTGAAAAACAAGCAGAAGTTTTATTAATGCGATTTTTACAAGGACAAACTAATAATTTCTCTAAAGATAATGTCAGTTTTTCAGTTGATTTATTTGGTTCTAACAGCGAATTTAGAAGTTGA", "aa": "MESNSSQAQVVTSNLLVSLSSKTTQKQQQKPVYVRGDDAIYAFHIDGGNYFLENSSPNKRNFEKQAEVLLMRFLQGQTNNFSKDNVSFSVDLFGSNSEFRS", "aa_hexdigest": "7dbc9403cfa7e941b6890b1c8db80b6b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11392.426599999992, "isoelectric_point": 6.553274345397949 }, "id": "JPCNHPFPBM_775", "locus": "GCA000292505_00771" }, { "type": "cds", "sequence": "contig_1", "start": 425324, "stop": 425464, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGAAGAGTGCTATGACAAAAAGTCAAGAAGTTACTTCAGACAATAATGGTTTTAATGTTAAAAGTGAATTTTTAAAAATTAATCCCTCGCTTTCTTCTTCTGGTAGTGATAATAGTAGTAACACGCAGTCTTTTTGA", "aa": "MMKSAMTKSQEVTSDNNGFNVKSEFLKINPSLSSSGSDNSSNTQSF", "aa_hexdigest": "6956f923ce8b266dcbd79b6fcabb3f5e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4963.360500000002, "isoelectric_point": 5.971186256408691 }, "id": "JPCNHPFPBM_776", "locus": "GCA000292505_00772" }, { "type": "cds", "sequence": "contig_1", "start": 425648, "stop": 425812, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTATTTTTACTAATTAAAACTTTGCTTGCACTATACTTGTTTTCAAAGTCAAGTAAATCATTTCTGGTATTAGTAGCAGCTGAACTTTGTGATGAACCACTAGAATTAGCAGTTTGTAATTGTTTATCAAACTCTTCAAATAATTTTGCAGAGTTTGTTTGA", "aa": "MLFLLIKTLLALYLFSKSSKSFLVLVAAELCDEPLELAVCNCLSNSSNNFAEFV", "aa_hexdigest": "29d48c29d2fb0bf6049ef0aff8176cfc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5974.032999999998, "isoelectric_point": 4.584314918518066 }, "id": "JPCNHPFPBM_777", "locus": "GCA000292505_00773" }, { "type": "cds", "sequence": "contig_1", "start": 425974, "stop": 426117, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATTCAAGTGATACAAGCGTTGCAACAACTAAAGAAAACATTGAAAAAACAGAAAATAAACTTAAGTTTATTAACAGCAATTTAAATGATGTAAATGGTGATTTTTTTACTAATCTTCAAGCTTATTTATTTTCACAGTGA", "aa": "MNSSDTSVATTKENIEKTENKLKFINSNLNDVNGDFFTNLQAYLFSQ", "aa_hexdigest": "19d2d05f462909308cc65baeeb6a2ea3", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5332.773000000001, "isoelectric_point": 4.593750190734863 }, "id": "JPCNHPFPBM_778", "locus": "GCA000292505_00774" }, { "type": "cds", "sequence": "contig_1", "start": 426370, "stop": 426573, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGTGCAACAGCAATAATTACAGCCTGTTCCACAATAAACACTGCTAATCTTTTTCCTACTCTCAATCGATCTCAAAAACTAATTGGTTTTAGTGATCAAAATATCATAAACCCAGATGTAGTTTTAAAGTCTGCTTTAAATGATGGTAGTGGTGTTGATTCTATCTTACGTGTTAGTTTTGGATCAGCATTACAAAAATGA", "aa": "MGATAIITACSTINTANLFPTLNRSQKLIGFSDQNIINPDVVLKSALNDGSGVDSILRVSFGSALQK", "aa_hexdigest": "8d840155f7d4cb0b7eb1aebb2784c033", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7008.939300000002, "isoelectric_point": 7.934011268615723 }, "id": "JPCNHPFPBM_779", "locus": "GCA000292505_00775" }, { "type": "cds", "sequence": "contig_1", "start": 426802, "stop": 427824, "strand": "-", "frame": 1, "gene": "recA", "product": "recombinase RecA", "db_xrefs": [ "COG:COG0468", "COG:L", "GO:0003677", "GO:0003684", "GO:0003697", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0008094", "GO:0009432", "GO:0016887", "GO:0140664", "KEGG:K03553", "RefSeq:WP_009885970.1", "SO:0001217", "UniParc:UPI00001334FA", "UniRef:UniRef100_P47581", "UniRef:UniRef50_P78014", "UniRef:UniRef90_P47581" ], "nt": "ATGGCTCAAAAAGAAATAATTAATAAGAAAAATACTCAAAAAAATAGTAGTTTTATTGAAAGTAATAATTTGACAAGTTTTGATTTTTTTGATGCAAAGAAAAACAGTGAAATTGAAACAATTTCAACTGGAAGTTTAAATTTAGATGAAGCATTAGGGTCTGGTGGTCTGCCTTTAGGTAGGATAGTAGAACTATATGGAAATGAATCATCTGGAAAAACAACTATTGCACTAAATGCAGTCGCTAGTTTTCAGAAAGCAGGTAAAACAGCCTGTTATATTGATGCTGAAGGTGCACTTGATTTAGCATATGCTAAATCAATTGGTATTGATCTAAATAAACTTTTGATTGCTCATCCTAGGCATGGTGAAAACGCTTTTGCTCTTATCGAATCATTAATTAAAACAAACAAGATATCTTTAATTGTTATTGACTCTGTAGCAGCGTTAATTCCTAAACAAGAGCTAGAAGGCACCATTGAAGAACAAACTATTGGCTTGCATGCAAGAATGATGTCAAAAGGTTTGCGAAGAATACAATCGATATTACCAGATTCTAAAACTTGTGTTTTATTCATTAATCAGTTACGCGAAAAACCAGGAGTGATGTTTGGAAATAACGAAGTTACAACAGGAGGAAAAGCTCTAAGATTTTATAGTTCATTAAGAATGGAAGCTAAGCGTGTTGAATTACTTAAGGATAAATTCAACAATTATGTTGGCATAAAAACAAAAGTAATGGTATCTAAAAATAAGATTGCTAAACCTTTTGGTGTTGCTATATTAGAAATCATGTTTAACCGTGGTTTTGTACATGAACATGAAGTTATTGACTTAGCACTTAAATTTAATGTTGTTGTAAGAGCTGGAAATTCTTATTCTTTTAACAATGAAAGCATTGCTGTTGGTAAAGAAAAATTATTAAATGTTTTATCAGAAAAGCCAGCATTATTTGAACAAATAAAAGAACTAACTGTTCAACAATTGGCTAATAAAAATTCATTTCAACAAACAGCTAGCTAA", "aa": "MAQKEIINKKNTQKNSSFIESNNLTSFDFFDAKKNSEIETISTGSLNLDEALGSGGLPLGRIVELYGNESSGKTTIALNAVASFQKAGKTACYIDAEGALDLAYAKSIGIDLNKLLIAHPRHGENAFALIESLIKTNKISLIVIDSVAALIPKQELEGTIEEQTIGLHARMMSKGLRRIQSILPDSKTCVLFINQLREKPGVMFGNNEVTTGGKALRFYSSLRMEAKRVELLKDKFNNYVGIKTKVMVSKNKIAKPFGVAILEIMFNRGFVHEHEVIDLALKFNVVVRAGNSYSFNNESIAVGKEKLLNVLSEKPALFEQIKELTVQQLANKNSFQQTAS", "aa_hexdigest": "de552d34d44dec5d67f423ae90a06d72", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001334FA", "ncbi_nrp_id": "WP_009885970.1", "uniref100_id": "UniRef100_P47581", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001334FA", "RefSeq:WP_009885970.1", "UniRef:UniRef100_P47581" ] }, "ips": { "uniref100_id": "UniRef100_P47581", "uniref90_id": "UniRef90_P47581" }, "psc": { "uniref90_id": "UniRef90_P47581", "gene": "recA", "product": "recombinase RecA", "uniref50_id": "UniRef50_P78014", "cog_id": "COG0468", "cog_category": "L", "kegg_orthology_id": "K03553", "go_ids": [ "GO:0003677", "GO:0003684", "GO:0003697", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0008094", "GO:0009432", "GO:0016887", "GO:0140664" ] }, "pscc": { "uniref50_id": "UniRef50_P78014", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78014" ], "product": "Protein RecA" }, "genes": [ "recA" ], "id": "JPCNHPFPBM_780", "locus": "GCA000292505_00776" }, { "type": "cds", "sequence": "contig_1", "start": 428160, "stop": 428336, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49391", "UniRef:UniRef90_UPI002FCE0920" ], "nt": "GTGGTGAAAAAGTATCAGTTAGATGTACCGAATGTTTCTAGCAACATGAACCAAGTGTTGCAAGAATTGATCCTTGAACAACCTTTGACTAAATATACCTTAAACAGTAGTTTGGCCAAAGAGAAGGGCAAAAGCCAAGTGGCGGTGCATCTGGGTAGTGGTCAAGCAAATCAGTGA", "aa": "MVKKYQLDVPNVSSNMNQVLQELILEQPLTKYTLNSSLAKEKGKSQVAVHLGSGQANQ", "aa_hexdigest": "97e3f1bf6884e78a444990e56b7bb478", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 428019, "stop": 428354, "observations": { "mutation": [ 428154, 428334 ], "stop": [ 428154, 428334 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FCE0920", "query_cov": 0.9827586206896551, "subject_cov": 0.4956521739130435, "identity": 0.93, "score": 99.8, "evalue": 6.57e-26, "gene_start": 1, "gene_end": 57, "reference_start": 50, "reference_end": 105, "reference_sequence": "MLFKSRDVKVKYESSGSNNISFENSNSQNNKPSYVVEFTNSTNVGIKWTMVKKYQLDVPNVSSDMNQVLQDLILEQPLTKYTLNGSLAKEKGKSQVAVHLGGQANNWQSMRHSMA" }, "paralog": false, "identity": 0.7982456140350878, "score": 162.0, "evalue": 2.98e-53, "description": "Internal stop codon at 428154, 428334. Nonsense mutation around 428154, 428334.", "psc": { "uniref90_id": "UniRef90_UPI002FCE0920", "uniref50_id": "UniRef50_Q49391", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FCE0920", "UniRef:UniRef50_Q49391" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6399.267600000003, "isoelectric_point": 9.158652687072756 }, "id": "JPCNHPFPBM_781", "locus": "GCA000292505_00777" }, { "type": "cds", "sequence": "contig_1", "start": 428343, "stop": 428444, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTAATCAACACAACTTAGACAACAATCCCAGTCCCAATGCATCAACCGGATTTAAATTAGACAAAGGCAACGCATATAGAAAATTAGATCAATCCTGA", "aa": "MRNQHNLDNNPSPNASTGFKLDKGNAYRKLDQS", "aa_hexdigest": "7224435ebd43099801c090085eb74984", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3732.0195000000012, "isoelectric_point": 9.520965003967284 }, "id": "JPCNHPFPBM_782", "locus": "GCA000292505_00778" }, { "type": "cds", "sequence": "contig_1", "start": 428464, "stop": 428619, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGACCAAGCAGGGCAAAGGGAAGGATAGTAGTGGGTGGAGTTCAACTGAAGAAACAACGGCAGAAAGGGATGCGCCCCTTGTTTCAACAAGTGGAACATCAGATCAAAGTAATAAATTCACCAAGTACCTCAACACCAAGCAAGCATTGGTAA", "aa": "MGPSRAKGRIVVGGVQLKKQRQKGMRPLFQQVEHQIKVINSPSTSTPSKHW", "aa_hexdigest": "e8949fb213f84d3364b45fffadca455a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5737.6705, "isoelectric_point": 11.999967765808105 }, "id": "JPCNHPFPBM_783", "locus": "GCA000292505_00779" }, { "type": "cds", "sequence": "contig_1", "start": 428613, "stop": 429065, "strand": "+", "frame": 3, "gene": "mgpC", "product": "MgpC family cytadherence protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030D3153C", "UniRef:UniRef90_UPI0030D3153C" ], "nt": "TTGGTAAGCATCGGTATCTTGTTTGATGAAGAGGAAATGGCGAGGAATGTTATCACGCAACTCTATTATGCTTCTACCAGTAAGCTAGCAGTCACTAACAACCACATTGTCGTGATGGGTAACAGCTTTCTACCCAGCTTGTGGTACTGGGTGGTGGATCGGGGTGCAACAACTGATTCATCATCAAAACCCACCTGGTTTGCTAACACCAATTTAGATTGGGGAGAAGACAAACAAAAACAATTTGTAGAGAACCAGTTGGGGTATAAGGAAACTACCAGTACCAATTCCCACAACTTCCATTCCAAATCTTTCACCCAACCAGCATATCTGATCAGTGGCATTGACAGTGTCAATGATCAGTTGATCTTCAGTGGTTTTAAAGCGGGGAGTGTGGGGTATGATAGTAGTAGTAGTAGTAGTACTCAAACCAAAGACCAAGCACTTGCTTGA", "aa": "MVSIGILFDEEEMARNVITQLYYASTSKLAVTNNHIVVMGNSFLPSLWYWVVDRGATTDSSSKPTWFANTNLDWGEDKQKQFVENQLGYKETTSTNSHNFHSKSFTQPAYLISGIDSVNDQLIFSGFKAGSVGYDSSSSSSTQTKDQALA", "aa_hexdigest": "12e7c22da29ff2f2b8e8dd12e28259b8", "start_type": "TTG", "rbs_motif": null, "genes": [ "mgpC" ], "truncated": "3-prime", "pseudogene": { "start": 428559, "stop": 429365, "observations": { "mutation": [ 429063, 429330, 429339 ], "stop": [ 429063, 429330, 429339 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0030D3153C", "query_cov": 0.9933333333333333, "subject_cov": 0.4901315789473684, "identity": 0.919, "score": 263.0, "evalue": 1.81e-86, "gene_start": 4, "gene_end": 150, "reference_start": 24, "reference_end": 171, "reference_sequence": "GGGGSDTASKFTKYLNTKQALERIGILFDGEKARNVITQLYYASTSKLAVTNNHIVVMGNSFLPSLWYWVVDRGATTDSSSKPTWFANTNLDWGEDKQKQFVENQLGYKETTSTNSHNFHSKSFTQPAYFISGIDSVNDQLIFSGFKAGSVGYDSSSSSSSSSSTKDQALAWSTTTSLDSKTGYTDLVTNDTGLNGPINGSFSIQDTFSFVVPYSGNHSNQTSSGPIKTAYPVKKSEASSEAINSLINATPLNSYGYRYIFLLWSHPKVEKRESSEGFKQRQWETQFNKVIHEWGYIHSQSPQG" }, "paralog": false, "identity": 0.8740740740740741, "score": 456.0, "evalue": 5.6e-165, "description": "Internal stop codon at 429063, 429330, 429339. Nonsense mutation around 429063, 429330, 429339.", "psc": { "uniref90_id": "UniRef90_UPI0030D3153C", "product": "MgpC family cytadherence protein", "uniref50_id": "UniRef50_UPI0030D3153C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0030D3153C", "UniRef:UniRef50_UPI0030D3153C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0030D3153C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0030D3153C" ], "product": "MgpC family cytadherence protein" } }, "id": "JPCNHPFPBM_784", "locus": "GCA000292505_00780" }, { "type": "cds", "sequence": "contig_1", "start": 429111, "stop": 429332, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05220.18" ], "nt": "GTGACCAACGACACGGGTTTAAATGGTCCAATCAATGGGAGTTTTTCAATCCAAGACACCTTCTCATTCGTTGTTCCTTATTCAGATAGTCATAGTAATCAAACTTCATCAGGACCCATTAAAACGGCTTATCCAGTGAAAAAAGATGAAGCTTCAAATGTAGCGATCAATTCTTTGATTAACGCTACGCCGTTGAATAGTTATGGAAGTGGAAGCAACTGA", "aa": "MTNDTGLNGPINGSFSIQDTFSFVVPYSDSHSNQTSSGPIKTAYPVKKDEASNVAINSLINATPLNSYGSGSN", "aa_hexdigest": "945498eb86fee2c656c0781561d8c9e0", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05220.18", "name": "MgpC", "length": 69, "aa_cov": 0.9452054794520548, "hmm_cov": 1.0, "evalue": 2.6491629586081833e-20, "score": 67.8019027709961, "start": 2, "stop": 70 } ], "seq_stats": { "molecular_weight": 7627.162400000001, "isoelectric_point": 4.74869327545166 }, "id": "JPCNHPFPBM_785", "locus": "GCA000292505_00781" }, { "type": "cds", "sequence": "contig_1", "start": 429313, "stop": 429609, "strand": "+", "frame": 1, "gene": null, "product": "MgPa", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49237" ], "nt": "ATGGAAGTGGAAGCAACTGATTCCTTTGATCCCACCCAAGGGTTGAAGAGTGATAGTCCAGTGAAGGATTCAGCAAGGGAAAGTCAAGAGAAACTCGAAACAACTGCTTCATCGATGAGTAGTGGTATGGCTACATCCACTCGCAAGGCCCTCAAAGTGGAGGTGGAAAAACAAAGTGAATCAACTGACGAACTTACAAACAACGACTTTGCTAAAAAGCCACTAAAGCATAAGAACAGTAGTGGGACAGATGTGAAGTTAGATTCACAGAAGGATTTTACTGAAGGAAAGGTTTGA", "aa": "MEVEATDSFDPTQGLKSDSPVKDSARESQEKLETTASSMSSGMATSTRKALKVEVEKQSESTDELTNNDFAKKPLKHKNSSGTDVKLDSQKDFTEGKV", "aa_hexdigest": "f94c33e3cc02c48f333e77418b812a44", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00027B3D10", "query_cov": 0.8571428571428571, "subject_cov": 0.8316831683168316, "identity": 0.619, "score": 78.6, "evalue": 4.99e-17, "valid": false, "uniref50_id": "UniRef50_Q49237" }, "pscc": { "uniref50_id": "UniRef50_Q49237", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49237" ], "product": "MgPa" }, "genes": [], "id": "JPCNHPFPBM_786", "locus": "GCA000292505_00782" }, { "type": "cds", "sequence": "contig_1", "start": 429616, "stop": 429849, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGACTACGGATGAGATAGAAAAAAATAGGGGGATGGGGATGCCAACTCCCCCTCCCCTTCAACTTCTGCTTCTTCTTCTTCTTCGAACTGGGGCTGGGTATCGCGTTCAAAGTGCTAATCAGAAAAACGGCATCCCTTTTGATAATGTGAAGCCTAGTAGTAGTAGTAGGTGGCTCCTCCAAACCAACCACCTACACCCATTTACCAAACTCCATCAGTCCCACCAGTGA", "aa": "MLTTDEIEKNRGMGMPTPPPLQLLLLLLLRTGAGYRVQSANQKNGIPFDNVKPSSSSRWLLQTNHLHPFTKLHQSHQ", "aa_hexdigest": "44fd632f7a175083d01a5c1b81db07c9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8700.9461, "isoelectric_point": 10.15610752105713 }, "id": "JPCNHPFPBM_787", "locus": "GCA000292505_00783" }, { "type": "cds", "sequence": "contig_1", "start": 429902, "stop": 430054, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTGTTAAGAGCACTGTTAGGAACTATTCCGGTATTGATCAATAAGAGCGGAGGAAGTGGGAATGAGTTTAACCATACGAATGATCAGAAGTGGGATAAAACGAATGAAAAAGATGGGAATTTACCTGGGTTTGGGGAGGTGAATGGGTGA", "aa": "MLLRALLGTIPVLINKSGGSGNEFNHTNDQKWDKTNEKDGNLPGFGEVNG", "aa_hexdigest": "d0db3fc5ae38df58538091d2ec99ff3e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5397.942400000002, "isoelectric_point": 5.564162254333495 }, "id": "JPCNHPFPBM_788", "locus": "GCA000292505_00784" }, { "type": "cds", "sequence": "contig_1", "start": 430078, "stop": 431601, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05000.22", "PFAM:PF04998.22" ], "nt": "GTGAAAGAAAAAGTATCTGATGAGATCCAAAGCTTAATTAAACAAGAACAGTACCGTGATAATTCCATTGTGGTAATGGCTGATTCAGGCGCTAGAGGTAACATTTCTAACTTTACCCAGTTATTTGGGATGCGCGGCTTAATGTCTAAAAGCTTTAACTATGAAAGAAATAACCAATCTAAGATCATTAAAGATACGATAGAAGTTCCTATTAAACACTCCTTTTTTGAAGGTTTGACCATTAATGAATACTTCAACTCTTCTTATGGAGCGAGAAAAGGGATGACAGATACTGCAATGAAAACAGCAAAGTCTGGTTATATGACAAGAAAGCTAGTAGATGCTACTCATGAATTAATTATTAACCATGATGATTGTGGAACAAGAAAAGGAATTGTTGTTGAAGCAATTGTTGAAACCAAAACCAAATCCTTGATTGAATCATTATTTGACAGGATTGTTAATCGCTACTCAATTACTCCTATAGTTGATCCTGAAACACAAAAAACTATTGTAGAAACTAACAGTCTTATTACAACGCAATTAGCTAAACAGATTTGTGCAACATCTATTAAGGAAGTTTTAGTTAGATCTGTTATCTATTGTGAAAGGGAAAATGGTATCTGTCAATACTGCTTTGGCATTGACTTGTCAACTGGTAAGTTGGTGGAATTGGGAACTGCTGTTGGGGTGATTGCTGCTCAATCAATTGGTGAACCTGGGACCCAATTGACAATGCGTACTTTCCATACTGGGGGAGTTTCAACTGAAAACAACTTAGCACAAGGCTTTGAACGTTTAAAACAGATCTTTGAAGTAGTTACTCCTAAAGATTTTGAAAAAGCAGTTATCTCTGAAGTGAAAGGAACAGTTAAATCAATTACTACTGTTCAAAACGCTCAGGAAGTAGTGATTAAATCAAACGTTGATGAGAGGATTTATACTATCCCTTTCAGTGCTCAAATACGTGTTCATGTTGGTGATCAAGTTTCACCAGGTTCTAAGATTACAGAAGGTTCTGTTGATATTAAACAACTTTTGCGAATTGCAGGTATCCAAAGGGTAAGACAATACATGATTGTAGAGATCCAAAAAGTGTATAGGATTCAAGGGATAGATATTGCTGATAAGTATGTAGAAATTATTATCAGACAACTAACTAATTTGTTGCAAGTAACAGATGCAGGTAACAGCAATTTATTTGTTGGTCAGTTAGTGCATAGCCATTATCTCAATGAACTAAATAAGAGCTTACTTTTAGCTGGAAAGATGCCTGTTATTGCAATTAATCAGGTGTTTGGAATTGATGAAGCGGCAAGTAAATCTAACTCCTTTTTAAGTGCTGCATCCTTCCAAGATACCAAAAAAATCCTAACTGATGCTGCTGTTAAGAACCAAGTAGACTATCTTTTAGGTTTAAAAGAGAATGTTATTATTGGTGGAAAAATTCCAGCAGGAACAGGATTTTTAACTGATGAAGAGTTAACTTTCTTAGGTAGCAAAACAGTTGCTGAAGAGTATTAA", "aa": "MKEKVSDEIQSLIKQEQYRDNSIVVMADSGARGNISNFTQLFGMRGLMSKSFNYERNNQSKIIKDTIEVPIKHSFFEGLTINEYFNSSYGARKGMTDTAMKTAKSGYMTRKLVDATHELIINHDDCGTRKGIVVEAIVETKTKSLIESLFDRIVNRYSITPIVDPETQKTIVETNSLITTQLAKQICATSIKEVLVRSVIYCERENGICQYCFGIDLSTGKLVELGTAVGVIAAQSIGEPGTQLTMRTFHTGGVSTENNLAQGFERLKQIFEVVTPKDFEKAVISEVKGTVKSITTVQNAQEVVIKSNVDERIYTIPFSAQIRVHVGDQVSPGSKITEGSVDIKQLLRIAGIQRVRQYMIVEIQKVYRIQGIDIADKYVEIIIRQLTNLLQVTDAGNSNLFVGQLVHSHYLNELNKSLLLAGKMPVIAINQVFGIDEAASKSNSFLSAASFQDTKKILTDAAVKNQVDYLLGLKENVIIGGKIPAGTGFLTDEELTFLGSKTVAEEY", "aa_hexdigest": "2bc37dad1763bb4745e7c390275d4190", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05000.22", "name": "RNA_pol_Rpb1_4", "length": 78, "aa_cov": 0.14595660749506903, "hmm_cov": 0.9871794871794872, "evalue": 9.977747000393254e-18, "score": 59.17518997192383, "start": 2, "stop": 75 }, { "id": "PF04998.22", "name": "RNA_pol_Rpb1_5", "length": 339, "aa_cov": 0.6331360946745562, "hmm_cov": 0.7581120943952803, "evalue": 2.0579555037077107e-58, "score": 192.97787475585938, "start": 78, "stop": 398 } ], "seq_stats": { "molecular_weight": 56108.682300000044, "isoelectric_point": 6.695030784606933 }, "id": "JPCNHPFPBM_789", "locus": "GCA000292505_00785" }, { "type": "cds", "sequence": "contig_1", "start": 431608, "stop": 433653, "strand": "-", "frame": 1, "gene": "rpoC", "product": "DNA-directed RNA polymerase subunit beta'", "db_xrefs": [ "COG:COG0086", "COG:K", "EC:2.7.7.6", "GO:0000287", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0008270", "KEGG:K03046", "SO:0001217", "UniRef:UniRef50_P47582", "UniRef:UniRef90_P47582" ], "nt": "ATGGGACATATTGCACTTGTGAGTCCTGTAGCTCACATTTGGATGTCAAAAGAATTACCATCTCCTTCCAAAATATCATTAGTTTTAAACATCTCTTACAAAGAGGTTGAACAGGTTTTGTACTTTGTTAACTACATAGTACTTGATACAGGTAAGATCAAAGATGATAAAATCATGCCTTTTAAGTTCAAAGAAGTTTTGGACTTAACTGGTAAGGGTTCACTTTCAACACGACAAAAAATGCGTCGTGTGATAGGTTATATCTTCAGAAATCTCATTAAAAGTAAGAGTAGTGAAGATTACCGTAAGGGAAAAATCTTTTATGAAAGTTTGAAAAACAGCTCTCTCCCCTTTTCTTTAAATGATGCTTTTAATTACATTAAGAAGTACACTGGTTTTAGGGTTGGAATAGGGGCTGAAGCAATTTTGGAATTGCTTAATAAAATCGATCTTAACTTGGAATTTAGCAGGTTAAATGATGCTTTAAGAAAAGCCAAGAAAGATAGTGTTGAAGATGCTAAAGTTAAGAAGATCTTAAGACAACTGGAAACTATTAGTTGGTTTAGAAATTCTAAGCTTCATCCTAAAAACATGATCTTACATACTGTTCCAGTTATCCCCCCTGATATCAGACCTATTATCCAACTTGATGGTGCTAAGTTTACCACCAGTGACATCAACAATTTTTACCGCAGGGTAATCATTAGAAATGACCGATTAAGAAGGATTTTGGAAGATGGTACTGTACCTTCTATTGTTGTTAACAATGAAAAAAGACTTTTACAAGAGTCTGTTGATGCTTTATTTGATAACTCTTCACGTCATAAACCATCACTTTCCAAAGACAAACGGTCATTGAAATCTTTAACAGATCGTTTAAAAGGAAAACAAGGTTTATTTAGACACAACTTACTTGGTAAAAGAGTTGATTATTCAGGTAGAAGTGTAATTGTGGTTGGCCCTGAATTGAAGATGTATGAAGTTGGGATCCCAGCACTAATGATCTTAAAGCTGTTTAAACCCTTTATTATCCATGGATTGATCAATAAGTTTGATGAAAATGGTAATGAGATTAGACCAATTGCCGCTTCCATCAGACAAGCTGAAGATATGATTAAAAACCAGGATGATCTTATCTGGGGAATAGTTTATGATGTTATCAAAGATCGTCCTGTTTTACTAAATCGTGCTCCAACCCTACATAGGTTAGGGATCCAAGCATTTGAACCAAGAATTGTTGATGGTAAAGCAATTAGATTACACCCATTAGTAACTACTGCATTTAATGCTGATTTTGATGGTGATCAGATGGCAGTTCATGTTCCTTTAAGTGATAATGCAGTTAATGAAGCAAGAGCTGTTCTGCTTGCATCAAAACATATCTTAGGTTTGAAAGATGGAAGACCCATTGTAACTCCTACTCAGGACATGGTTTTAGGTAACTATTATCTAACAACAGAGAGAAAAGAACAGTTGGGAGAGGGGATTATCTTCAGCACAGTTTATGAAGCACGTGCTGCTTATGAAAGTCAAAAGGTTCATTTACATGCTATTGTAGGGATAAGTACTAAAGCATTTCCCAATAAGAAGTTTGCATGCCAAGGAACTTTAATAACAACAGTTGGTAAGATTATCTTTAATGATGTTTTAGGCAATAATGTTCCTTATATTAATGACGGGGAATTTGATGAAAATGCATGTCCTGAAAAGTTCATTGTGAAACAGGGAGAAGATGTAAGACAAGCAATTTTAAAGCATCAAATTATCCCTGCATTTTCCAAAAAGGTTATTTCCAAGTTAATTGATCTACTCTATCTTTTATTGGAATTTAAAGATCTTCCTAAAACACTTGATAATATCAAAGCACTTGGCTTTAAGTACTCTACTTTTTCTTCAACTACTGTTTCAGTATTTGATATCCCTAAGTACACTAATAAACAAAATTACTTTGATAGTGCTGATCAACAGGTGCTGAAATACAAACAGTTTTATAACAAGGGTTTGTTAACCGATGATGAACGTTATAAACGCGTTGTGAAGTTATGA", "aa": "MGHIALVSPVAHIWMSKELPSPSKISLVLNISYKEVEQVLYFVNYIVLDTGKIKDDKIMPFKFKEVLDLTGKGSLSTRQKMRRVIGYIFRNLIKSKSSEDYRKGKIFYESLKNSSLPFSLNDAFNYIKKYTGFRVGIGAEAILELLNKIDLNLEFSRLNDALRKAKKDSVEDAKVKKILRQLETISWFRNSKLHPKNMILHTVPVIPPDIRPIIQLDGAKFTTSDINNFYRRVIIRNDRLRRILEDGTVPSIVVNNEKRLLQESVDALFDNSSRHKPSLSKDKRSLKSLTDRLKGKQGLFRHNLLGKRVDYSGRSVIVVGPELKMYEVGIPALMILKLFKPFIIHGLINKFDENGNEIRPIAASIRQAEDMIKNQDDLIWGIVYDVIKDRPVLLNRAPTLHRLGIQAFEPRIVDGKAIRLHPLVTTAFNADFDGDQMAVHVPLSDNAVNEARAVLLASKHILGLKDGRPIVTPTQDMVLGNYYLTTERKEQLGEGIIFSTVYEARAAYESQKVHLHAIVGISTKAFPNKKFACQGTLITTVGKIIFNDVLGNNVPYINDGEFDENACPEKFIVKQGEDVRQAILKHQIIPAFSKKVISKLIDLLYLLLEFKDLPKTLDNIKALGFKYSTFSSTTVSVFDIPKYTNKQNYFDSADQQVLKYKQFYNKGLLTDDERYKRVVKL", "aa_hexdigest": "ce3c7b2b380cf502b0810e2c0f874445", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpoC" ], "truncated": "both", "pseudogene": { "start": 431305, "stop": 433953, "observations": { "mutation": [ 431610, 433686, 433860 ], "stop": [ 431610, 433686, 433860 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47582", "query_cov": 1.0, "subject_cov": 0.5270897832817337, "identity": 0.996, "score": 1319.0, "evalue": 0.0, "gene_start": 1, "gene_end": 681, "reference_start": 102, "reference_end": 782, "reference_sequence": "MTTTRRNKRNNKLYKNIKAIKLSIASNDTILNWSEGEVTKAETINYKSLKPEPGGLFDEAIFGPVKDYECACGKFKKIKYRGVRCDRCGVWVTESIVRRERMGHIALVSPVAHIWMSKELPSPSKISLVLNISYKEVEQVLYFVNYIVLDTGKIKDDKIMPFKFKEVLDLTGKGSLSTRQKMRRVIGYIFRNLIKSKSSEDYRKGKIFYESLKNSSLPFSLNDAFNYIKKYTGFRVGIGAEAILELLNKIDLNLEFSRLNDALRKAKKDSVEDAKVKKILRQLETISWFRNSKLHPKNMILHTVPVIPPDIRPIIQLDGAKFTTSDINNFYRRVIIRNDRLRRILEDGTVPSIVVNNEKRLLQESVDALFDNSSRHKPSLSKDKRSLKSLTDRLKGKQGLFRHNLLGKRVDYSGRSVIVVGPELKMYEVGIPALMILKLFKPFIIHGLINKFDENGNEIRPIAASIRQAEDMIKNQDDLIWGIVYDVIKDRPVLLNRAPTLHRLGIQAFEPRIVDGKAIRLHPLVTTAFNADFDGDQMAVHVPLSENAVNEARAVLLASKHILGLKDGRPIVTPTQDMVLGNYYLTTERKGQLGEGIIFSTVYEARAAYESQKVHLHAIVGISTKAFPNKKFACQGTLITTVGKIIFNDVLGNNVPYINDGEFDENACPEKFIVKQGEDVRQSILKHQIIPAFSKKVISKLIDLLYLLLEFKDLPKTLDNIKALGFKYSTFSSTTVSVFDIPKYTNKQNYFDSADQQVLKYKQFYNKGLLTDDERYKRVVKLWNNVKEKVSDEIQNLIKQEQYRDNSIVVMADSGARCNISNFTQLFGMRGLMSKSFNYERNNQSKIIKDTIEVPIKHSFFEGLTINEYFNSSYGARKGMTDTAMKTAKSGYMTRKLVDATHELIINHDDCGTRKGIVVEAIVETKTKSLIESLFDRIVNRYSITPIVDPETQKTIVEANSLITTQLAKQICATSIKEVLVRSVIYCERENGICQYCFGIDLSTGKLVELGTAVGVIAAQSIGEPGTQLTMRTFHTGGVSTENNLAQGFERLKQIFEVVTPKDFEKAVISEVKGTVKSITTVQNAQEVVIKSNVDERIYTIPFSAQIRVHVGDQVSPGSKITEGSVDIKQLLRIAGIQRVRQYMIVEIQKVYRIQGIDIADKYVEIIIRQLTNLLQVTDAGNSNLFVGQLVHSHYLNELNKSLLLAGKMPVIAINQVFGIDEAASKSNSFLSAASFQDTKKILTDAAVKNQVDYLLGLKENVIIGGKIPAGTGFLTDEELTFLGSKTVAEEY" }, "paralog": false, "identity": 0.9909297052154195, "score": 1719.0, "evalue": 0.0, "description": "Internal stop codon at 431610, 433686, 433860. Nonsense mutation around 431610, 433686, 433860.", "psc": { "uniref90_id": "UniRef90_P47582", "gene": "rpoC", "product": "DNA-directed RNA polymerase subunit beta'", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P47582", "cog_id": "COG0086", "cog_category": "K", "kegg_orthology_id": "K03046", "go_ids": [ "GO:0000287", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0008270" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47582", "EC:2.7.7.6", "UniRef:UniRef50_P47582", "COG:COG0086", "COG:K", "KEGG:K03046", "GO:0000287", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0008270" ] }, "pscc": { "uniref50_id": "UniRef50_P47582", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47582" ], "product": "DNA-directed RNA polymerase subunit beta'" } }, "id": "JPCNHPFPBM_790", "locus": "GCA000292505_00786" }, { "type": "cds", "sequence": "contig_1", "start": 433858, "stop": 433956, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAACAACAAGACGTAATAAAAGAAATAACAAGCTTTATAAAAACATTAAAGCAATTAAACTTTCCATCGCTTCCAATGACACCATTTTGAACTGA", "aa": "MTTTRRNKRNNKLYKNIKAIKLSIASNDTILN", "aa_hexdigest": "05503160a2613d897970a8709258a958", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3734.3353000000006, "isoelectric_point": 11.165940284729004 }, "id": "JPCNHPFPBM_791", "locus": "GCA000292505_00787" }, { "type": "cds", "sequence": "contig_1", "start": 433966, "stop": 434235, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04560.25" ], "nt": "TTGCAAGAACTTTTAACCATTAAATCTGATGATGTACAAGGAAGAAATAGGGCTTATGCTGCTATTGTTAAAGGTGCAGCTTTCCCAGAGCCTGGTATCCCTGAATCATTTAAATTATTGACAAAAGAATTACAGGGCTTGGCTTTATCTGTTTCATTTATCTATGATGACAACACCCAACAAGACTCCAATAATGTTTCCATCTTGCAAAGTGATGGGGAACAAGATGAATTTTTCAATGATTTTGAATTTGACACTGAGGGTTATTAG", "aa": "MQELLTIKSDDVQGRNRAYAAIVKGAAFPEPGIPESFKLLTKELQGLALSVSFIYDDNTQQDSNNVSILQSDGEQDEFFNDFEFDTEGY", "aa_hexdigest": "7ad708e8f50a0819af35d6c2caab9c68", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04560.25", "name": "RNA_pol_Rpb2_7", "length": 64, "aa_cov": 0.6067415730337079, "hmm_cov": 1.0, "evalue": 3.5128374197727186e-13, "score": 44.94830322265625, "start": 1, "stop": 54 } ], "seq_stats": { "molecular_weight": 9971.774399999997, "isoelectric_point": 4.0500284194946286 }, "id": "JPCNHPFPBM_792", "locus": "GCA000292505_00788" }, { "type": "cds", "sequence": "contig_1", "start": 434266, "stop": 435342, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00562.33" ], "nt": "TTGTCTACTAAAAATGGTGATGAACAAATTACCCGTGATATCCCTAATGTTAGTGATGCAAACAAACGCTATCTTGATGAGAATGGCATCATTATGGTGGGTGCTGAAGTTAAAGAAGGGGATGTTTTGGTTGGTAAAGTTTCCCCTAAAGGTCAAGTCGAAGTCTCTCCTGAAGAAAAGCTATTTAAAGCCATCTTCCCTGAAAGTGTTCAAAACGTGAGAGACTCTTCACTTAAAGTTCCCCATGGTGGGGATGGTATTGTTTCAGCTGTAAAACGTTTTTCAATTGCTAATGGTGATGAACTTAATGATGGTGTGATTGAAATGATCAAGGTTTATGTGGTTCAAAAACGTAAGATTCAAATTGGTGATAAATTAGCTGGTAGACACGGAAATAAAGGAGTTATTTCTAAAGTGGTGCCTATTGAAGATATGCCCCATTTAGAAGATGGAACCCCAGTTGATATTCTACTCAACCCCCTTGGTGTTCCTAGTCGGATGAACATAGGACAAATTTTTGAAACCCACTTGGGTTATGCAGCACACAAGCTAGCAGTTCGTTCTTTAATTAGTAGTTGTTTTGATCAAAATAAAGCTAAGGAGTTTGCCATTGAAATCAATCAACCTCAAGCAAGGGTTGAAAGATTAATTAAAGGTTTAAAAAGCCAAATCAATGATCGCAATATTAAAAGTGAAAAAGAAGCACTTGAAAAACTCGATAACAGTGACATTAGTTTAGTTTTGAAAGAGATAGGGATGTCTTTTGATGATCTTATTTACAAAATTGCAACCCCTATTTTCCAAGGAGTGAACTTCTTAGATCTCCAAGATGTTATGCAAGAAGCAGGATTAGATCCCCAAAAAAATCAGGGTAAGTTTAAACTCATTGATGGTAGGAGTGGAATGCCATTTGAAAGACCTATTTCACTTGGAATTATGTACATGATGAAGCTGAATCACATGGTTGATGATAAGATCCATGCTCGTGCTGTTGGCCCTTATTCTAAGATCACCCAACAACCATTAGGTGGTAAATCGCAAAATGGTGGTCAGCGGTTTGGTGAGATGGAAGTGTGA", "aa": "MSTKNGDEQITRDIPNVSDANKRYLDENGIIMVGAEVKEGDVLVGKVSPKGQVEVSPEEKLFKAIFPESVQNVRDSSLKVPHGGDGIVSAVKRFSIANGDELNDGVIEMIKVYVVQKRKIQIGDKLAGRHGNKGVISKVVPIEDMPHLEDGTPVDILLNPLGVPSRMNIGQIFETHLGYAAHKLAVRSLISSCFDQNKAKEFAIEINQPQARVERLIKGLKSQINDRNIKSEKEALEKLDNSDISLVLKEIGMSFDDLIYKIATPIFQGVNFLDLQDVMQEAGLDPQKNQGKFKLIDGRSGMPFERPISLGIMYMMKLNHMVDDKIHARAVGPYSKITQQPLGGKSQNGGQRFGEMEV", "aa_hexdigest": "b0d0200ffa32cc819bd5fe1be07362ea", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00562.33", "name": "RNA_pol_Rpb2_6", "length": 351, "aa_cov": 0.9636871508379888, "hmm_cov": 0.7435897435897436, "evalue": 2.8790473942396325e-95, "score": 314.5927429199219, "start": 3, "stop": 347 } ], "seq_stats": { "molecular_weight": 39556.035700000044, "isoelectric_point": 6.421294212341309 }, "id": "JPCNHPFPBM_793", "locus": "GCA000292505_00789" }, { "type": "cds", "sequence": "contig_1", "start": 435442, "stop": 438138, "strand": "-", "frame": 1, "gene": "rpoB", "product": "DNA-directed RNA polymerase subunit beta", "db_xrefs": [ "COG:COG0085", "COG:K", "EC:2.7.7.6", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549", "KEGG:K03043", "SO:0001217", "UniRef:UniRef50_P47715", "UniRef:UniRef90_P47583" ], "nt": "ATGTCACAAAAATCTAATTTCTTTCAAAAACGTTATTCCCCTACAGCTACCAGAAGGTATTATGGCAAAATTGAGACCAATTTTATCCAACCAAATTTAGCTGATATTCAGATTAAAAGCTACCAAAAATTCTTAGATCATGATCTTGAAAAATTAATTGCCTCATATTTTCCAATCAAATCCCCTAATGATCGCTACACTATCAATTTTAGGGGATTACACAGAACTGAACCAGAACGTGATGAAGCACAATCACGTGCTCAATCTAAAACTTATGAAGTTGGTATTTATGCTGATCTTGAATTAGTTGATAATGATAAAGGAACAGTTAAAAAAGCACGGAAATCAAAGAAAAATATTGCTAGCAATACAAATGGTGTATTTTTAGCTAACATGCCTTTAATAACCCATGATGGGGTTTTTATTATCAATGGGATAGAAAAGTTTGTTATTTCCCAAATAACCCGTTCTCCAGGGATATACATGCTAACAAAATCCCAACTAAAACTATCCAACTCCCGTAAAAGAGTACAGGAAGGTTATGTTTGTGAGGTTTTACCTGCTAATGGTTCAGTGATGCTTATATACATCTCCAATAAAAAAAAGATTGAAGATGCTTTTGTACAAATTCTTTTAAGAGATGCGGTAAGAGAAGGTGCTAAGATTTTCCCTATTACAACACTTTTAAAAGCGTTTGGTTTAAATAATCGTGAGATCCTTAAAATCTTTAAAAACAATGAATTTATCAAACGTTCATTGGAAGCGGAAATTTACAATGCTAAAGATTTTTTAAGCAATGTTGATCCTGAAATCAAAAACCTTTTAAAAGAATTTAGAGATGGTAAAACTGATTTAAGAAGAAAAGGGATCGCTTCTGATCAGAAATTAAGATCACTTGTTAATGAATATGTAACGCTTGAGAAACAATATAATGCGCTAAAACAAACAAGTCCAAATGATTCTAGTTTAACTGCACTTGAACTGGAAATGGAAAACAAAATGGATAGTGTTATTACTGAAAGAGCTGCTAAACACATTGTCAATGAACTTTCTATTTCACTGCGTGATATTGAAAACACTGAAGAGTGTCATGAAGTGAGTTTCCATGCACTTTTATGTGCGCGTTTTTTTAGAAACAAGAGGTACAACCTCTCTAATGCAGGGAGATACAAAGTATCTAGAAAGTTACGTTTAACAGAACGTATTTATCAAAAAACTCTGGCATGTGATTTGTTCTTAAAAGATGGCAAGCTACTTTTAAAAAAAGGTACTTTACTTTTAAAAGAAGAGATTGACAAAATCAAACAAGCTGCTAAGAACAATGAAATTAGTTTTGTTAATAAAATGCAACTTACAACTGATGGTAAGGCTGTTGATTTAGCAAAAGAATCACTCTTTTATGAAACGATAGATGTATATATCACTAATGATAATCTTAGTGTTTCAGTACCAGTTATAGGGATCCATAACGAAAATGATCTGAACAAAGCAATGACTCTCAGTGATTTCATCGCTTCAATTAGTTATGTGATTAATTTACCTTATGGAATTGGTAAATATGATGATATTGATCACCTTGGTAATAAGCGGGTTAAGTTAATTAATGAATTAATTACTGCTAAATTAGAAAGCGGCTTCACTAGAATGGAGCGCTTTTTAAAAGAAAAGTTAACTATTGCTGATGGAGTTAACCGTGGCCAGCAAATTAATGAAGAGGGTCAGGTTATTGAACAAGGTGAAAAAAAGGAATTAACTATTAAATCTTTAATCAACTCAAAACCAATTCAAATTGTGATTAAAGACTTCTTCAATACCCACCAATTAACCCAATTTTTAGACCACCAAAACCCTTTATCAGAATTGAGTAATAAAAGAAGGATTTCAGCAATGGGACCTGGGGGAATATCAAGAGAGGACCCTAATTTAGATATCCGTGATGTGCATTATTCTCAGTACGGTAGAATTTGCCCTATTGAAACACCTGAAGGGATGAACATAGGGTTGATCATGTCTTTAGCTAGCTTTGCTAAGATTGATGAAAACGGATTTTTAATGGCACCTTATCGCAAAATCAAAGCTGGGGTAATTACTGATGAGGTGGAATATTTAACTGCGCTTAGAGAAGATGAACATATTATTGCTGAGATCTCTTCACTTGTCAATATTAGTAACGATAACAAGATCTTAGATAAGGAAATTATTGGTAGGTATCGATCTATGCAAGGACTTTATGATCCTTTAAAGATTGATTACATTGATGTAGCACCACACCAAGTTGTTTCCATTGGTTCTTCTTTAATCCCCTTTTTGGAAAATGATGATTCAGCTAGAGCATTAATGGGAACCAACATGCAACGTCAGGCCTATCCTTTAATAAAGCCATATGCTCCTGCAGTAGGTACTGGTCAAGAACACAAAATTGCTAGTGATTCAGGTTTAACAATGTCCTCTCCTTGCTCAGGTGTTGTTAGTTATGTTGATAACAGTAAGATTATTATTACAAGTGATAGTTCTAAAAAAGAGACAGTTAACCTAGTTAAATTTGAACGTTCCAACCAAAATACTTGTTATAACCACAAACCAATTGTTGAAATAGGCCAAAGGGTTAATAAGGATGAAATCATTGTTGATGGCCCTGCTGTTAATAAGAGTGAGTTGGCATTAGGACAGAATGTTTTAGTTGCTTTTACAACTTGA", "aa": "MSQKSNFFQKRYSPTATRRYYGKIETNFIQPNLADIQIKSYQKFLDHDLEKLIASYFPIKSPNDRYTINFRGLHRTEPERDEAQSRAQSKTYEVGIYADLELVDNDKGTVKKARKSKKNIASNTNGVFLANMPLITHDGVFIINGIEKFVISQITRSPGIYMLTKSQLKLSNSRKRVQEGYVCEVLPANGSVMLIYISNKKKIEDAFVQILLRDAVREGAKIFPITTLLKAFGLNNREILKIFKNNEFIKRSLEAEIYNAKDFLSNVDPEIKNLLKEFRDGKTDLRRKGIASDQKLRSLVNEYVTLEKQYNALKQTSPNDSSLTALELEMENKMDSVITERAAKHIVNELSISLRDIENTEECHEVSFHALLCARFFRNKRYNLSNAGRYKVSRKLRLTERIYQKTLACDLFLKDGKLLLKKGTLLLKEEIDKIKQAAKNNEISFVNKMQLTTDGKAVDLAKESLFYETIDVYITNDNLSVSVPVIGIHNENDLNKAMTLSDFIASISYVINLPYGIGKYDDIDHLGNKRVKLINELITAKLESGFTRMERFLKEKLTIADGVNRGQQINEEGQVIEQGEKKELTIKSLINSKPIQIVIKDFFNTHQLTQFLDHQNPLSELSNKRRISAMGPGGISREDPNLDIRDVHYSQYGRICPIETPEGMNIGLIMSLASFAKIDENGFLMAPYRKIKAGVITDEVEYLTALREDEHIIAEISSLVNISNDNKILDKEIIGRYRSMQGLYDPLKIDYIDVAPHQVVSIGSSLIPFLENDDSARALMGTNMQRQAYPLIKPYAPAVGTGQEHKIASDSGLTMSSPCSGVVSYVDNSKIIITSDSSKKETVNLVKFERSNQNTCYNHKPIVEIGQRVNKDEIIVDGPAVNKSELALGQNVLVAFTT", "aa_hexdigest": "cf4db95a6cd8473f08a6ad3ca80c4fc2", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpoB" ], "truncated": "5-prime", "pseudogene": { "start": 435139, "stop": 438138, "observations": { "mutation": [ 435444 ], "stop": [ 435444 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47583", "query_cov": 1.0, "subject_cov": 0.6460431654676259, "identity": 0.9990000000000001, "score": 1719.0, "evalue": 0.0, "gene_start": 1, "gene_end": 898, "reference_start": 1, "reference_end": 898, "reference_sequence": "MSQKSNFFQKRYSPTATRRYYGKIETNFIQPNLADIQIKSYQKFLDHDLEKLIASYFPIKSPNDRYTINFRGLHRTEPERDEAQSRAQSKTYEVGIYADLELVDNDKGTVKKARKSKKNIASNTNGVFLASMPLITHDGVFIINGIEKFVISQITRSPGIYMLTKSQLKLSNSRKRVQEGYVCEVLPANGSVMLIYISNKKKIEDAFVQILLRDAVREGAKIFPITTLLKAFGLNNREILKIFKNNEFIKRSLEAEIYNAKDFLSNVDPEIKNLLKEFRDGKTDLRRKGIASDQKLRSLVNEYVTLEKQYNALKQTSPNDSSLTALELEMENKMDSVITERAAKHIVNELSISLRDIENTEECHEVSFHALLCARFFRNKRYNLSNAGRYKVSRKLRLTERIYQKTLACDLFLKDGKLLLKKGTLLLKEEIDKIKQAAKNNEISFVNKMQLTTDGKAVDLAKESLFYETIDVYITNDNLSVSVPVIGIHNENDLNKAMTLSDFIASISYVINLPYGIGKYDDIDHLGNKRVKLINELITAKLESGFTRMERFLKEKLTIADGVNRGQQINEEGQVIEQGEKKELTIKSLINSKPIQIVIKDFFNTHQLTQFLDHQNPLSELSNKRRISAMGPGGISREDPNLDIRDVHYSQYGRICPIETPEGMNIGLIMSLASFAKIDENGFLMAPYRKIKAGVITDEVEYLTALREDEHIIAEISSLVNISNDNKILDKEIIGRYRSMQGLYDPLKIDYIDVAPHQVVSIGSSLIPFLENDDSARALMGTNMQRQAYPLIKPYAPAVGTGQEHKIASDSGLTMSSPCSGVVSYVDNSKIIITSDSSKKETVNLVKFERSNQNTCYNHKPIVEIGQRVNKDEIIVDGPAVNKSELALGQNVLVAFTTWNGYNYEDAIVISERLVKEDILTSLTINEYVAQCLSTKNGDEQITRDIPNVSDANKRYLDENGIIMVGAEVKEGDVLVGKVSPKGQVEVSPEEKLFKAIFPESVQNVRDSSLKVSHGGDGIVSAVKRFSIANGDELNDGVIEMIKVYVVQKRKIQIGDKLAGRHGNKGVISKVVPIEDMPHLEDGTPVDILLNPLGVPSRMNIGQIFETHLGYAAHKLAVRSLISSCFDQNKAKEFAIEINQPQARVERLIKGLKNQINDRNIKSEKEALEKLDNSDISLVLKEIGMSFDDLIYKIATPIFQGVNFLDLQDVMQEAGLDPQKNQGKFKLIDGRSGMPFERPISLGIMYMMKLNHMVDDKIHARAVGPYSKITQQPLGGKSQNGGQRFGEMEVWALEAYGAAYNLQELLTIKSDDVQGRNRAYAAIVKGAAFPEPGIPESFKLLTKELQGLALSVSFIYDDNTQQDSNNVSILQSDGEQDEFFNDFEFDTEGY" }, "paralog": false, "identity": 0.997997997997998, "score": 1930.0, "evalue": 0.0, "description": "Internal stop codon at 435444. Nonsense mutation around 435444.", "psc": { "uniref90_id": "UniRef90_P47583", "gene": "rpoB", "product": "DNA-directed RNA polymerase subunit beta", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P47715", "cog_id": "COG0085", "cog_category": "K", "kegg_orthology_id": "K03043", "go_ids": [ "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47583", "EC:2.7.7.6", "UniRef:UniRef50_P47715", "COG:COG0085", "COG:K", "KEGG:K03043", "GO:0000428", "GO:0001054", "GO:0001055", "GO:0001056", "GO:0001057", "GO:0001058", "GO:0001066", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0032549" ] }, "pscc": { "uniref50_id": "UniRef50_P47715", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47715" ], "product": "DNA-directed RNA polymerase subunit beta" } }, "id": "JPCNHPFPBM_794", "locus": "GCA000292505_00790" }, { "type": "cds", "sequence": "contig_1", "start": 438228, "stop": 438734, "strand": "-", "frame": 2, "gene": "ssuE", "product": "putative protein MG342", "db_xrefs": [ "COG:C", "COG:COG0431", "GO:0005524", "GO:0005829", "GO:0010181", "GO:0016491", "RefSeq:WP_014894675.1", "SO:0001217", "UniParc:UPI00027B3D3B", "UniRef:UniRef100_UPI00027B3D3B", "UniRef:UniRef50_P75270", "UniRef:UniRef90_P47584" ], "nt": "ATGTCAAAAGATCAAAAGTCATTAATTTTAATGCTCTCCAATTCGCAACATTCCATTAACAGGAAATTTGCTAATGAATTAAAACATTCTTTAAGTTGTGAATTGATTGAGTTAAAAGACTATCAAGTTGATTTTTATTCTGTTGATTTAGAAGCAACTAACTTTCCTGACAAAATTAAAACTTTAGTTAGAAAAATCAAAGAACATAGCAATTTAATTTTTGTTACCCCTGAACACAACGGTTTTATCCCTGCTTTTGCTAAAAACATCATTGACTGGATGACTCGTGATGAGCAGTATGGTAGAAATCAATTTTTAAAAGGTCTTAATGGAGTTATTTGTTGTGTAACTCCAGCAACTGCGGGTGGGGATAAAACTGTTCTAGAATTACTAGATAAATTTTTAAGTTTTAGCGGTCTTAATGTTAAAGGAACAGTTTTAGTCAACGGTTATCATGAGAACTTTGATTTTAAGCCTTTTATTGAACAAGTTAAAAAATTAATATAA", "aa": "MSKDQKSLILMLSNSQHSINRKFANELKHSLSCELIELKDYQVDFYSVDLEATNFPDKIKTLVRKIKEHSNLIFVTPEHNGFIPAFAKNIIDWMTRDEQYGRNQFLKGLNGVICCVTPATAGGDKTVLELLDKFLSFSGLNVKGTVLVNGYHENFDFKPFIEQVKKLI", "aa_hexdigest": "a8330af8a46cb528a664abe50acd5aa4", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3D3B", "ncbi_nrp_id": "WP_014894675.1", "uniref100_id": "UniRef100_UPI00027B3D3B", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3D3B", "RefSeq:WP_014894675.1", "UniRef:UniRef100_UPI00027B3D3B" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3D3B", "uniref90_id": "UniRef90_P47584" }, "psc": { "uniref90_id": "UniRef90_P47584", "gene": "SsuE", "product": "Uncharacterized protein MG342", "uniref50_id": "UniRef50_P75270", "cog_id": "COG0431", "cog_category": "C", "go_ids": [ "GO:0005829", "GO:0005524", "GO:0010181", "GO:0016491" ] }, "pscc": { "uniref50_id": "UniRef50_P75270", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75270" ], "product": "Uncharacterized protein MG342 homolog" }, "genes": [ "ssuE" ], "id": "JPCNHPFPBM_795", "locus": "GCA000292505_00791" }, { "type": "cds", "sequence": "contig_1", "start": 438813, "stop": 438995, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTTTGATTTGGAATTCCACTTAATTGTTGAAATAATGAAAGGTTTTAATTTTGATGAGAATTGTATCTGTTTACTTGAAGGAAATAAAGAACAAAACCCCAATTTACCAACCGTTAGTTTAGATACTAAGGTTGCTGATATTAAGACTTATCAAGGTTGCTATTTTCTCAATAATTAA", "aa": "MIFDLEFHLIVEIMKGFNFDENCICLLEGNKEQNPNLPTVSLDTKVADIKTYQGCYFLNN", "aa_hexdigest": "c9af8b3911cbb767f1463288a6edf938", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6944.911500000004, "isoelectric_point": 4.395041084289551 }, "id": "JPCNHPFPBM_796", "locus": "GCA000292505_00792" }, { "type": "cds", "sequence": "contig_1", "start": 439092, "stop": 439487, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAATAGTTTTTTTATTAATTAATTCACCAGAAGAAAAAACAACTATTAAGCTTTCTGATTCGCTAATTAAAGAAATTAAAAAACAAAAAATAAAACATGAAGTTTATTCTTTAAGATCATTTCAAAATAAGTTTTTTAAGTTAGTTAGAAAATTAGAAAAAAAGCACAAGTCTAAAAATAACGTAATGTTTGTTGAACTAAACGGTGTGTTTGGTTATGAAACTAACTTTGAAAAAAGTAACATTGATTTTAGTTTCACTAGTTTTCAAGACAGATTCACAATTGAAAAAAAGTTAAAAATCACCAGCCATTTTATCTTGCCTCATAAATACTTGTTAAAAAATTTAGAAAACATTAAGCATCCTAACTTTGAGCAAAAGAACTTAATTTGA", "aa": "MKIVFLLINSPEEKTTIKLSDSLIKEIKKQKIKHEVYSLRSFQNKFFKLVRKLEKKHKSKNNVMFVELNGVFGYETNFEKSNIDFSFTSFQDRFTIEKKLKITSHFILPHKYLLKNLENIKHPNFEQKNLI", "aa_hexdigest": "6cf12a82c5c16982bc8a65fc2ac0d402", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15738.292599999992, "isoelectric_point": 9.8639368057251 }, "id": "JPCNHPFPBM_797", "locus": "GCA000292505_00793" }, { "type": "cds", "sequence": "contig_1", "start": 439614, "stop": 439853, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTCATAAAATTAATGATCAAACCCTCGTTAATGAGCAACGCCTATTAGCTTATGATTTTTTTCAAAATAGCAATAAGGTTGGACTTTTATCAACATTACAATATCTAGATTTTGTTAATTTTTTAGTTAGATCTAAAAAAGTAAATTATCTGCTTGTTAACAAAGTAAGTCTTCCAATATATCAAAAGATTTATTTTGATAACTTCCCTTTTTTAGGTTTTGATAATAACTTGTGA", "aa": "MSHKINDQTLVNEQRLLAYDFFQNSNKVGLLSTLQYLDFVNFLVRSKKVNYLLVNKVSLPIYQKIYFDNFPFLGFDNNL", "aa_hexdigest": "cfd8b67c0df74949332d557a25b5fc2e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9368.678999999995, "isoelectric_point": 9.00418643951416 }, "id": "JPCNHPFPBM_798", "locus": "GCA000292505_00794" }, { "type": "cds", "sequence": "contig_1", "start": 439857, "stop": 440141, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAAAAGCTTTCTAATAATATTTTGAAAGAACTACCAGTTTTAAATAAGACTTATAAAAATTTAAAGTTACCAGTGTTTTTGGTTTTAGCTCAAAATGATTTATTTATGCCAACAAAATTAACATTAAGCTATTTCAATAAATACCTAATTAAGAACAATAATTTGCAATCATCTGTTATCTTAAACAGTGAACACCAAATGTTTAATAGTAAATATGAAAGCTTTTGTAAAGCAATGGATGATATCCTGAATCATAATAAACTAAGTAAAATTTATTAA", "aa": "MAKKLSNNILKELPVLNKTYKNLKLPVFLVLAQNDLFMPTKLTLSYFNKYLIKNNNLQSSVILNSEHQMFNSKYESFCKAMDDILNHNKLSKIY", "aa_hexdigest": "9eedfe8da8d8eb0270fad1306074968c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11015.864299999994, "isoelectric_point": 9.608835411071777 }, "id": "JPCNHPFPBM_799", "locus": "GCA000292505_00795" }, { "type": "cds", "sequence": "contig_1", "start": 440169, "stop": 440378, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGCTGCTGTTTGTAGTTATGCCATGAATTTATTAAATGCTAAACGTGTTGAAAAACTCATACTTTTAGCACCTCTTTCATACTGTAACTTATTGAGATATTTCAAAATCAAAAGTTCTTTTAAAAAAGATAAGGCAGAAAGAATGGCTAATTTTAAAGCAATGTTTCAAACTAAATTTAGTAATTTAACTGATGAAAACAGCTGA", "aa": "MGAAVCSYAMNLLNAKRVEKLILLAPLSYCNLLRYFKIKSSFKKDKAERMANFKAMFQTKFSNLTDENS", "aa_hexdigest": "53fff15810565e49c23b518d71d95a70", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7917.299999999997, "isoelectric_point": 9.745379447937012 }, "id": "JPCNHPFPBM_800", "locus": "GCA000292505_00796" }, { "type": "cds", "sequence": "contig_1", "start": 440845, "stop": 441060, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAACCTCTTTACAAAACCGACTGTTTTTAAAGTTGCTAACTCTAAGAATTTAGAAAATATCTATAAAACTTTTACTAGTATTAAAAATGCTGCTTTCAAAGAAATTGAAAAGCTAAGAAAAGAAGGGTTGATTTCTAAAAATAATCAAATTGAATTAACCGTTGGAATTAATAAAAAAATACCCAAAAAATTAAAGGATAATCTCGCACTTTGA", "aa": "MNLFTKPTVFKVANSKNLENIYKTFTSIKNAAFKEIEKLRKEGLISKNNQIELTVGINKKIPKKLKDNLAL", "aa_hexdigest": "68d5682c9058b674823a24a2206c3b2e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8121.540599999993, "isoelectric_point": 10.056761741638184 }, "id": "JPCNHPFPBM_801", "locus": "GCA000292505_00797" }, { "type": "cds", "sequence": "contig_1", "start": 441253, "stop": 441390, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGATTATAAGTTTTCACTAGAAGACAAAATAGTTATCCATAAAACTAATTCACTAGTAGAACAAATTGAGAAATTTTTAGAGAAATATAATTTTTTAGGTTGCCTAAAAGTGATTAATAAGTTTGTTTTATGA", "aa": "MDDYKFSLEDKIVIHKTNSLVEQIEKFLEKYNFLGCLKVINKFVL", "aa_hexdigest": "6c768a02f337ced0c35d4b18980563ae", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5394.306199999999, "isoelectric_point": 6.534574317932129 }, "id": "JPCNHPFPBM_802", "locus": "GCA000292505_00798" }, { "type": "cds", "sequence": "contig_1", "start": 441529, "stop": 441774, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "TTGGAAATAAATAAATATGGTTCAATAGCTGATCTTTATATTGAAGGTTCTGATCAATATCGGGGTTGGTTCAACTCTTCTTCAAATTGCGGAATTATTCAAAATGATTTAATCCCTTTTAAATCACTTGTTTCACATGGTTTTACACTTGATGAAAATGGCAATAAAATGTCAAAGTCATTAGGAAACATAGTTGATCCTTTAAAAATTTGTGATCAATATGGAGCGGATATATTAAGGTTGTGA", "aa": "MEINKYGSIADLYIEGSDQYRGWFNSSSNCGIIQNDLIPFKSLVSHGFTLDENGNKMSKSLGNIVDPLKICDQYGADILRL", "aa_hexdigest": "4bb8445ed36ea9a405cc1e4836f8a11b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 72, "aa_cov": 0.8888888888888888, "hmm_cov": 1.0, "evalue": 1.1544175247913003e-26, "score": 88.53526306152344, "start": 10, "stop": 81 } ], "seq_stats": { "molecular_weight": 9033.086399999993, "isoelectric_point": 4.791606712341309 }, "id": "JPCNHPFPBM_803", "locus": "GCA000292505_00799" }, { "type": "cds", "sequence": "contig_1", "start": 442180, "stop": 442452, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAATACATGTTGTTGATAATGAGGGAACAGGTATTGTTCACAGCTCCCCTGCATTTGGAATTGATGATTTTTATCTTTGTCAAAAAAACAAGATTAAAGAAGTTTTGATTTCTATTGATGAGAAAGGTGTATTTAATAACTTACTTAATGATAAAGAACTTGAGAATTGTTTTTATCTTAAAGCAAATGATCTAATTATTAATCGTTTAAAACAAAACAATAGCTTTATTTTTTCTGAAGTTATTTCCCACCGCGAACCACATGATTGA", "aa": "MGIHVVDNEGTGIVHSSPAFGIDDFYLCQKNKIKEVLISIDEKGVFNNLLNDKELENCFYLKANDLIINRLKQNNSFIFSEVISHREPHD", "aa_hexdigest": "6224d419f170cbc0dc5f76aa7e67cbae", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10306.548399999989, "isoelectric_point": 5.225913429260252 }, "id": "JPCNHPFPBM_804", "locus": "GCA000292505_00800" }, { "type": "cds", "sequence": "contig_1", "start": 442819, "stop": 443184, "strand": "-", "frame": 1, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0029621904", "UniRef:UniRef90_UPI0029621904" ], "nt": "GTGGGACATGCTCTTAACAAGATTTTAAAAGACTTCATTTTACGTAGTTGGTTATATGAAGGATATGATGTTGTTTTTATTCCTGGTTGGGATTGTCATGGACTACCAATAGAACATGCAGTTAGTAAGAAAAACCCTAGTAGTTATAGCAATCTTTCAACTGTTGAAAAAAGAAAATTATGTCATCAGTTTGCACTTTCACAAATTGCAGTTCAAAAAGAACAATTTCAAAGACTGGGACTTTTAAATGATTTTCAAAACTGTTATTACACAATAGATGAGAGTTTTCAATTTAAGGAACTTGAACTATTTTTACAAGCAATTAAAAAAGGGCTCATTTTTCAAGATTTAAAACCAACTTATTGA", "aa": "MGHALNKILKDFILRSWLYEGYDVVFIPGWDCHGLPIEHAVSKKNPSSYSNLSTVEKRKLCHQFALSQIAVQKEQFQRLGLLNDFQNCYYTIDESFQFKELELFLQAIKKGLIFQDLKPTY", "aa_hexdigest": "7e4deceb4c8eb1dcf961f4576070d4c5", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 442778, "stop": 443232, "observations": { "deletion": [ 442857 ], "stop": [ 442791 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0029621904", "query_cov": 0.9752066115702479, "subject_cov": 0.7712418300653595, "identity": 0.941, "score": 230.0, "evalue": 6.68e-76, "gene_start": 1, "gene_end": 118, "reference_start": 17, "reference_end": 134, "reference_sequence": "MKILHDGPPYANGSIHVGHALNKILKDFILRSWLYEGYDVVFIPGWDCHGLPIEHAVSKKNPSSYSNLSTVEKRKLCHQFALSQIAVQKEQFQRLGLLNDFQNCYYTIDESFQFKELELFLQAIKKRAHFSRFKTNLLITNFKNFTCWSGNWI" }, "paralog": false, "identity": 0.9867549668874173, "score": 306.0, "evalue": 2.56e-108, "description": "Internal stop codon at 442791. Frameshift due to deletion around 442857.", "psc": { "uniref90_id": "UniRef90_UPI0029621904", "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_UPI0029621904", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0029621904", "UniRef:UniRef50_UPI0029621904" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0029621904", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0029621904" ], "product": "class I tRNA ligase family protein" } }, "id": "JPCNHPFPBM_805", "locus": "GCA000292505_00801" }, { "type": "cds", "sequence": "contig_1", "start": 443420, "stop": 443587, "strand": "+", "frame": 2, "gene": "spoU", "product": "tRNA/rRNA methyltransferase SpoU type domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A917CEE2" ], "nt": "ATGTATAAATCAGTAATAAACATAGTTTTATTTTGTCCAGAAATTCCTAATAACACTGGCAACATCGTACGTAGTTGCACTGCTTTTAAAGCTAATCTACACTTAATTAAACCTTATGGCTTTTTCTTAAATGAAAAAAGGATGGTTAGAGCTGGTTTAAATTGTTGA", "aa": "MYKSVINIVLFCPEIPNNTGNIVRSCTAFKANLHLIKPYGFFLNEKRMVRAGLNC", "aa_hexdigest": "dc61a0a8e9915c28b46f329b176c9fbe", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0023B9359B", "query_cov": 0.9090909090909091, "subject_cov": 0.819672131147541, "identity": 0.6, "score": 64.3, "evalue": 1.41e-12, "valid": false, "product": "tRNA (cytidine(34)-2'-O)-methyltransferase", "ec_ids": [ "2.1.1.207" ], "uniref50_id": "UniRef50_A0A917CEE2", "kegg_orthology_id": "K03216" }, "pscc": { "uniref50_id": "UniRef50_A0A917CEE2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A917CEE2" ], "product": "tRNA/rRNA methyltransferase SpoU type domain-containing protein" }, "genes": [ "spoU" ], "id": "JPCNHPFPBM_806", "locus": "GCA000292505_00802" }, { "type": "cds", "sequence": "contig_1", "start": 443705, "stop": 443818, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAATAAATTACCAAACGAACTTTACTTTGTTTTTGGTCAGGAAACAAAGGGATTACCTAAAACAATCATGGATAACTTTAAACAAAACCAAATTAGAATTCCCATTTGA", "aa": "MTNKLPNELYFVFGQETKGLPKTIMDNFKQNQIRIPI", "aa_hexdigest": "b71224ab4b6b21218cc93ad966e91af1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4368.0843, "isoelectric_point": 9.398732948303223 }, "id": "JPCNHPFPBM_807", "locus": "GCA000292505_00803" }, { "type": "cds", "sequence": "contig_1", "start": 443911, "stop": 444252, "strand": "+", "frame": 1, "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "db_xrefs": [ "COG:COG0220", "COG:J", "EC:2.1.1.33", "GO:0006400", "GO:0008176", "GO:0030488", "GO:0036265", "GO:0043527", "SO:0001217", "UniRef:UniRef50_P47589", "UniRef:UniRef90_P47589" ], "nt": "GTGCGCTTAAGAAAAGTTAAAAACGCTCTTTTAAAAATTAATCAAAGTCCTTATTTTTATTCAAAAGATAAGTTTGCTAAGTTTACTAAAAAACAATTAGTGCTGGAATTGGGTTGTGGTAAGGGTACTTTTTTAATCAAAGAAGCACAAAAAAATAACAATTTTCTTTTTATAGGAATTGAACGTGAACCTACAATTGTTTTAAAAGCAATTAACAAAATTAACAAGTTGGATTTTAATTTGGAAAATATCTTATTGTTGTGTACAGATGCAAAACAACTTGATGATTATTTTCAAGCTGAATCTGTTCAAAAAATCTTTATTAATTTCCCTGATCCTTGA", "aa": "MRLRKVKNALLKINQSPYFYSKDKFAKFTKKQLVLELGCGKGTFLIKEAQKNNNFLFIGIEREPTIVLKAINKINKLDFNLENILLLCTDAKQLDDYFQAESVQKIFINFPDP", "aa_hexdigest": "ebf3cc8848cdb8fad7990322f4f92b93", "start_type": "GTG", "rbs_motif": null, "genes": [ "trmB" ], "truncated": "3-prime", "pseudogene": { "start": 443911, "stop": 444543, "observations": { "mutation": [ 444250, 444310 ], "stop": [ 444250, 444310 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47589", "query_cov": 1.0, "subject_cov": 0.5380952380952381, "identity": 1.0, "score": 224.0, "evalue": 4.29e-73, "gene_start": 1, "gene_end": 113, "reference_start": 1, "reference_end": 113, "reference_sequence": "MRLRKVKNALLKINQSPYFYSKDKFAKFTKKQLVLELGCGKGTFLIKEAQKNNNFLFIGIEREPTIVLKAINKINKLDFNLENILLLCTDAKQLDDYFQAESVQKIFINFPDPWPKKRHIQRRLTSPDFLKLFWNLLVKNGLIEFKTDNDKLFEYTLTTLQENSQIFEIIHQITDLNNSEFSFQNSITEYEQRFMELEIPIKKLVIKKII" }, "paralog": false, "identity": 0.9857142857142858, "score": 407.0, "evalue": 9.82e-148, "description": "Internal stop codon at 444250, 444310. Nonsense mutation around 444250, 444310.", "psc": { "uniref90_id": "UniRef90_P47589", "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "ec_ids": [ "2.1.1.33" ], "uniref50_id": "UniRef50_P47589", "cog_id": "COG0220", "cog_category": "J", "go_ids": [ "GO:0006400", "GO:0008176", "GO:0030488", "GO:0036265", "GO:0043527" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47589", "EC:2.1.1.33", "UniRef:UniRef50_P47589", "COG:COG0220", "COG:J", "GO:0006400", "GO:0008176", "GO:0030488", "GO:0036265", "GO:0043527" ] }, "pscc": { "uniref50_id": "UniRef50_P47589", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47589" ], "product": "tRNA (guanine-N(7)-)-methyltransferase" } }, "id": "JPCNHPFPBM_808", "locus": "GCA000292505_00804" }, { "type": "cds", "sequence": "contig_1", "start": 444388, "stop": 444543, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGAAAATAGTCAAATTTTTGAAATTATCCATCAAATAACTGATCTTAACAATTCTGAATTCAGTTTTCAAAATAGTATTACTGAATATGAACAGCGCTTTATGGAATTAGAAATTCCAATTAAAAAACTAGTGATTAAGAAAATAATTTAA", "aa": "MQENSQIFEIIHQITDLNNSEFSFQNSITEYEQRFMELEIPIKKLVIKKII", "aa_hexdigest": "7a662a05397a943d6bd9c36add1aa56d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6172.043699999997, "isoelectric_point": 4.876922035217285 }, "id": "JPCNHPFPBM_809", "locus": "GCA000292505_00805" }, { "type": "tRNA", "sequence": "contig_1", "start": 444576, "stop": 444651, "strand": "+", "gene": "trnH", "product": "tRNA-His(gtg)", "amino_acid": "His", "anti_codon": "gtg", "score": 71.9, "nt": "GCGATTGTGGCGAAGTGGTTAACGCACCTGATTGTGGATCAGGCATTCGTGGGTTCAATTCCCATCAGTCGCCCCA", "db_xrefs": [ "SO:0000262" ], "anti_codon_pos": [ 444582, 444584 ], "id": "JPCNHPFPBM_15", "locus": "GCA000292505_00806" }, { "type": "cds", "sequence": "contig_1", "start": 444694, "stop": 445215, "strand": "-", "frame": 1, "gene": null, "product": "putative lipoprotein MG348", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47590", "UniRef:UniRef90_P47590" ], "nt": "GTGGATAGAAAAAACGAACTTGCAAGTCAAGAAGATAAAGACAATTCCAATAAGTTAAGAAGATCAGATGCTTCTGCAGTTCGCTATAGAGAAATAGTTGATTTTATTCAACGTACTTATGGTGGTAATGTAGCTAACTTAATCAATCAATCAAATGTGCATGCTCAAACAGTTGGTAATGATGTAACTAAAGCAATTGTGATTGCTTTTAGAAAAGATAACCTTAATAAGATAAGAGCTAATTTTTATGGTTTGGATAACTCTACAAATCCTAATGCACCTGGATCAGGTCAGGGTGATTCAACCCCACCAGCTTCAAGTGGTGAAGGGGGCGGTAGTGATGGCAGTTCAGGTGGTGACTCAAGTAGTGGTAATGGACAAAATACTACTCCTACTTCACCACAATCTTCTCAACCAGCAGTTCAAAGATCACAAAAATCTTATGGAATTAAACAACACGCAGTAAGAGTTAGTGTTGATTTTTTAAATTTCTTGGATAGTGTTTATACTCCACTAAACTAG", "aa": "MDRKNELASQEDKDNSNKLRRSDASAVRYREIVDFIQRTYGGNVANLINQSNVHAQTVGNDVTKAIVIAFRKDNLNKIRANFYGLDNSTNPNAPGSGQGDSTPPASSGEGGGSDGSSGGDSSSGNGQNTTPTSPQSSQPAVQRSQKSYGIKQHAVRVSVDFLNFLDSVYTPLN", "aa_hexdigest": "9738ae84d53004541df9d7f7c059d0ac", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 444694, "stop": 445515, "observations": { "mutation": [ 445242, 445353 ], "stop": [ 445242, 445353 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47590", "query_cov": 1.0, "subject_cov": 0.5372670807453416, "identity": 0.9940000000000001, "score": 295.0, "evalue": 4.14e-98, "gene_start": 1, "gene_end": 173, "reference_start": 150, "reference_end": 322, "reference_sequence": "MKRLFWNLKHKKAWLVLLLGTGMILSSCSNIDQSFFRELSVESVEKNTAYNQLPVNSTTFRNLVFGTRSYNDGNYVVVIATETDSSQINFLNGSTNQGTSSLSWGGTLGTTIRQVQNRYSTYPNGVKFLIWNDIAPGSVKWNPYARFPVVDRKNELASQEDKDNSNKLRRSDASAVRYREIVDFIQRTYGGNVANLINQSNVHAQTVGNDVTKAIVIAFRKDNLNKIRANFYGLDNSTNPNAPGSGQGDSTPPASSGEGGGSDGSSGGDSSSGNGQNTTPTSPQSSQPAVQRSQKSYGIKQHAVRVSVDFLNFLDSVYTPLN" }, "paralog": false, "identity": 0.9926739926739927, "score": 510.0, "evalue": 1.85e-185, "description": "Internal stop codon at 445242, 445353. Nonsense mutation around 445242, 445353.", "psc": { "uniref90_id": "UniRef90_P47590", "product": "Uncharacterized lipoprotein MG348", "uniref50_id": "UniRef50_P47590", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47590", "UniRef:UniRef50_P47590", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47590", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47590" ], "product": "Uncharacterized lipoprotein MG348" } }, "id": "JPCNHPFPBM_810", "locus": "GCA000292505_00807" }, { "type": "cds", "sequence": "contig_1", "start": 445351, "stop": 445596, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTAAGTTCTTGTAGTAACATCGATCAGTCTTTTTTTCGTGAACTTTCTGTTGAAAGTGTGGAAAAAAATACAGCTTACAACCAATTGCCTGTTAACAGTACAACATTTAGAAACTTAGTTTTTGGAACCAGATCATATAACGATGGTAATTATGTGGTTGTTATTGCAACCGAAACAGATAGTTCCCAAATTAATTTTCTAAACGGTTCTACTAATCAAGGTACAAGTTCTCTTAGTTGA", "aa": "MILSSCSNIDQSFFRELSVESVEKNTAYNQLPVNSTTFRNLVFGTRSYNDGNYVVVIATETDSSQINFLNGSTNQGTSSLS", "aa_hexdigest": "c86275855398790d5a21928bf08b0e14", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8910.615799999998, "isoelectric_point": 4.440682792663575 }, "id": "JPCNHPFPBM_811", "locus": "GCA000292505_00808" }, { "type": "tRNA", "sequence": "contig_1", "start": 445675, "stop": 445762, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(gag)", "amino_acid": "Leu", "anti_codon": "gag", "score": 63.4, "nt": "GTCGGAGTGGTGGAATGGTAGACACGCAAGCTTGAGGTGCTTGTGGTCGTTAAAGACTGTGCCAGTTCAAGTCTGGTCTCCGACACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 445756, 445758 ], "id": "JPCNHPFPBM_18", "locus": "GCA000292505_00809" }, { "type": "cds", "sequence": "contig_1", "start": 446114, "stop": 446230, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACCCAACTATTACCGTGTGATCAAAAAAGCAACATCATTTTCTGGATTAGAAATGATCTCTCATGCTTATGGTCAAATTGCGGGAGTGGAAGTTGTTGGGTTTTATTTATGA", "aa": "MQPNYYRVIKKATSFSGLEMISHAYGQIAGVEVVGFYL", "aa_hexdigest": "0915135e70d0a1e36d478ffd60a4f290", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4268.9082, "isoelectric_point": 8.119873619079591 }, "id": "JPCNHPFPBM_812", "locus": "GCA000292505_00810" }, { "type": "cds", "sequence": "contig_1", "start": 446384, "stop": 446626, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGAACTTTTTTTTCACCAAAACGCTCTGAAATAACTTTTTGTATTATTGATCCTTTAGATTGGAAAGAATTTAAGCAAAACAAACAATTAAAAGAAAAACTAGTTGAGGCAATGGGAAAAGTTGAATATGACCGAAACTGCTTAGCTTTTGATCAAATCGACAATCTTCAATTTGATAATGCGCTTGAAATCTCTGCTAACTTTGAAGTTAATTTCACTGCAAACCAAAGCGATGTTTGA", "aa": "MRTFFSPKRSEITFCIIDPLDWKEFKQNKQLKEKLVEAMGKVEYDRNCLAFDQIDNLQFDNALEISANFEVNFTANQSDV", "aa_hexdigest": "ce70d05047fff2972ce81ab9881046c1", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9427.553499999996, "isoelectric_point": 4.678099250793457 }, "id": "JPCNHPFPBM_813", "locus": "GCA000292505_00811" }, { "type": "cds", "sequence": "contig_1", "start": 446744, "stop": 447082, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAACAAATTACTGATTGCATCATCAACAGTACTCAAGAGAATGAGCTTGATTTTCAAAAGTTAGAAATGATGTTTTTTCAAATAGTAAAAAATGATACAGCTCCCATTTTAGAAACAGTTTCAAACAACAAAGACTTTTTTTATAAGAATGAAATTTTAGATGAATCAACAAAAAAAGCAATAACAGACTGTCATGTAAACTTCAATTCTGAAAAATACCTTTTTCTTCTATATGGAAAGATAGATGAATCGCAATTGCAATTAGTTAGACAATTGAGAAGTGATTATCAACTATTAGATAAAGTAATTAATTTAGTATTAGACTTTTCCTTTTGA", "aa": "MQQITDCIINSTQENELDFQKLEMMFFQIVKNDTAPILETVSNNKDFFYKNEILDESTKKAITDCHVNFNSEKYLFLLYGKIDESQLQLVRQLRSDYQLLDKVINLVLDFSF", "aa_hexdigest": "6b6e6d89afb77e2fdca0f6031c37bd01", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13271.977299999988, "isoelectric_point": 4.549472618103027 }, "id": "JPCNHPFPBM_814", "locus": "GCA000292505_00812" }, { "type": "cds", "sequence": "contig_1", "start": 447283, "stop": 447498, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTTCACCAGATCAAAAAGATAAAAAGTCATTATTTAAAAAACATTTTGCTTATTTGAAATTAACTGAAAAAGAACTTAATGATCCTAAGATACAAACACTTTTAGAAGTTGCGTGGAATCATTTTGATCAGTGTAGAAAAATTAAAGAAAGAAGATGTAGTAATAAAGGTGATATCCATCTTCAAGCTGTTAGAGAGTGGTTACCTTGA", "aa": "MKISPDQKDKKSLFKKHFAYLKLTEKELNDPKIQTLLEVAWNHFDQCRKIKERRCSNKGDIHLQAVREWLP", "aa_hexdigest": "55370079a5ce544a8a31a663ee684bed", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8563.8949, "isoelectric_point": 9.553714942932128 }, "id": "JPCNHPFPBM_815", "locus": "GCA000292505_00813" }, { "type": "cds", "sequence": "contig_1", "start": 447682, "stop": 447942, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTTGATTCCAATGATCCAAAATTTACCAGTAACTCCAAGTGATAAAAATAGTTTTGGTTATGCATACAAAAAGATATCTAATCTTTTTGAAACTGAAAAGAAAACAAAGAAAAGGATATTTTTTTTCAATTTACAAAAGAATAACATTAACAATATGGCAGCTTGTATGTTAACTTGTGAAATAGCTAAGAAAAATATAAAAGTTGCTTTAATTTATTGTGAAGAATTTGTTAGCAGATATGACAAAAGTTATTGA", "aa": "MDLIPMIQNLPVTPSDKNSFGYAYKKISNLFETEKKTKKRIFFFNLQKNNINNMAACMLTCEIAKKNIKVALIYCEEFVSRYDKSY", "aa_hexdigest": "f509a4fcb46e1b13e096fd9f505fc189", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10100.777699999995, "isoelectric_point": 9.324723243713382 }, "id": "JPCNHPFPBM_816", "locus": "GCA000292505_00814" }, { "type": "tRNA", "sequence": "contig_1", "start": 448276, "stop": 448357, "strand": "+", "gene": "trnL", "product": "tRNA-Leu(caa)", "amino_acid": "Leu", "anti_codon": "caa", "score": 59.5, "nt": "CCTGGAGTGGCGGAATGGTAGACGCGGTGGACTCAAAACCCACTAGGAAACTGTAGGAGTTCAAGTCTCCTCTCCAGGACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 448309, 448311 ], "id": "JPCNHPFPBM_16", "locus": "GCA000292505_00815" }, { "type": "cds", "sequence": "contig_1", "start": 448901, "stop": 449110, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGTAATAAACTGGTTTTTTTGCCAAAAAAATCATACCATCAGTTATTTAATATTACTTTTAGTGCAATGATGCTTGCTTTGGCCATCATTACATCATTAATTAGTGAATTTATTAGTATTCCTTTTTTCTCACAACTAAAACTAAATTTTGATATTTCCGTTGTTTTTTTAGTTGCATGTGCTTTTTTTGTGTCACTAGGTTGA", "aa": "MNGNKLVFLPKKSYHQLFNITFSAMMLALAIITSLISEFISIPFFSQLKLNFDISVVFLVACAFFVSLG", "aa_hexdigest": "6e29e4dd51e8106ada634a7a1228fca8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7738.238999999994, "isoelectric_point": 9.035582542419434 }, "id": "JPCNHPFPBM_817", "locus": "GCA000292505_00816" }, { "type": "cds", "sequence": "contig_1", "start": 449158, "stop": 449319, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00719.24" ], "nt": "ATGGACAAATTTTTAATTGATGTTATTGTAGAAATCCCTAAAAACAGCAAAATAAAGTATGAGTATGATCGTCAAACTGGTCAAATTCGCGTTGATAGAATCCTATTTGGAAGTGAATCATATCCACAAAACTACGGTTTTATTAAAAATACATTAGATTGA", "aa": "MDKFLIDVIVEIPKNSKIKYEYDRQTGQIRVDRILFGSESYPQNYGFIKNTLD", "aa_hexdigest": "1e81362bdd10d07a3c9581d7b9a7943b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00719.24", "name": "Pyrophosphatase", "length": 45, "aa_cov": 0.8490566037735849, "hmm_cov": 1.0, "evalue": 8.94418658106683e-18, "score": 59.66098403930664, "start": 8, "stop": 52 } ], "seq_stats": { "molecular_weight": 6300.1115, "isoelectric_point": 6.145397758483886 }, "id": "JPCNHPFPBM_818", "locus": "GCA000292505_00817" }, { "type": "cds", "sequence": "contig_1", "start": 449380, "stop": 449598, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00719.24" ], "nt": "GTGCCTACAAGAATTGTAGGAGCACTTGAGATGATTGATGATGGGGAAATTGATACTAAGTTATTAGGAGTTATTGATTGTGACCCTAGATATAAAGAAATTAATCAAATTAGTGATTTACCTAAACATAGAATAGAAGAAATTCTTATCTTTTTAAAAACTTATAAATTACTTCAAAAAAAGACTGTAATTATTAAGGGTTTAAAAGATGTTTGTTGA", "aa": "MPTRIVGALEMIDDGEIDTKLLGVIDCDPRYKEINQISDLPKHRIEEILIFLKTYKLLQKKTVIIKGLKDVC", "aa_hexdigest": "acb30d4e67b259dd1258e2fa1f954240", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00719.24", "name": "Pyrophosphatase", "length": 69, "aa_cov": 0.9583333333333334, "hmm_cov": 1.0, "evalue": 3.884632196426609e-19, "score": 64.08428192138672, "start": 2, "stop": 70 } ], "seq_stats": { "molecular_weight": 8282.800799999994, "isoelectric_point": 6.551682853698731 }, "id": "JPCNHPFPBM_819", "locus": "GCA000292505_00818" }, { "type": "cds", "sequence": "contig_1", "start": 449907, "stop": 450215, "strand": "+", "frame": 3, "gene": "recU", "product": "Holliday junction resolvase RecU", "db_xrefs": [ "COG:COG3331", "COG:R", "EC:3.1.21.10", "GO:0000287", "GO:0003676", "GO:0004519", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0007059", "KEGG:K03700", "SO:0001217", "UniRef:UniRef50_Q49422", "UniRef:UniRef90_Q49422" ], "nt": "ATGTATTTTGATTTTGAAGCAAAACAAACAAATAAAAGTAATTTTCCAATTGCTCAAATAGCAGAACACCAATTAAATCATTTGAAAAGAATTGACCAAATTGGAGGAGTTAGCTTCTTGCTAATTTACTTTCAAACTAAAGATCAAATTTTTGCATTTCACACTAAGGATCTTCTAGAAACAATAAAAAATCAAGAAAGCAAAACAATTAAAAGGGAATTGATAGAACAAAAATCTCAGAAAGTTCCACTATTGTATCCTGGTATTATTGATTTAATTTCAATAATTCAAAGCTTTAAAAATTATTAG", "aa": "MYFDFEAKQTNKSNFPIAQIAEHQLNHLKRIDQIGGVSFLLIYFQTKDQIFAFHTKDLLETIKNQESKTIKRELIEQKSQKVPLLYPGIIDLISIIQSFKNY", "aa_hexdigest": "765350889a981d7f6d3b937009619494", "start_type": "ATG", "rbs_motif": null, "genes": [ "recU" ], "truncated": "5-prime", "pseudogene": { "start": 449715, "stop": 450215, "observations": { "mutation": [ 449796 ], "stop": [ 449796 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49422", "query_cov": 1.0, "subject_cov": 0.6144578313253012, "identity": 1.0, "score": 202.0, "evalue": 3.09e-65, "gene_start": 1, "gene_end": 102, "reference_start": 65, "reference_end": 166, "reference_sequence": "MINANRGMLLETIVNQTIARLKDHPDIWLEKRFLPIKPIAFRHAHVSGNVSQKSKTDYYGIYKGMYFDFEAKQTNKSNFPIAQIAEHQLNHLKRIDQIGGVSFLLIYFQTKDQIFAFHTKDLLETIKNQESKTIKRELIEQKSQKVPLLYPGIIDLISIIQSFKNY" }, "paralog": false, "identity": 0.9939759036144579, "score": 328.0, "evalue": 3.05e-117, "description": "Internal stop codon at 449796. Nonsense mutation around 449796.", "psc": { "uniref90_id": "UniRef90_Q49422", "gene": "recU", "product": "Holliday junction resolvase RecU", "ec_ids": [ "3.1.21.10" ], "uniref50_id": "UniRef50_Q49422", "cog_id": "COG3331", "cog_category": "R", "kegg_orthology_id": "K03700", "go_ids": [ "GO:0000287", "GO:0003676", "GO:0004519", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0007059" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49422", "EC:3.1.21.10", "UniRef:UniRef50_Q49422", "COG:COG3331", "COG:R", "KEGG:K03700", "GO:0000287", "GO:0003676", "GO:0004519", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0007059" ] }, "pscc": { "uniref50_id": "UniRef50_Q49422", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49422" ], "product": "Holliday junction resolvase RecU" } }, "id": "JPCNHPFPBM_820", "locus": "GCA000292505_00819" }, { "type": "cds", "sequence": "contig_1", "start": 450227, "stop": 450541, "strand": "-", "frame": 3, "gene": "hupA", "product": "putative MG353-like protein", "db_xrefs": [ "COG:B", "COG:COG0776", "GO:0003677", "GO:0030527", "SO:0001217", "UniRef:UniRef50_P47595", "UniRef:UniRef90_P47595" ], "nt": "ATGGAAAAAACATCAAATACAAGTAAGCCACTTTCTCGTAGTGAAATCAATAAAATAATTGCAGTTGCTACTGGTATAAAAGAAAAAAAAATTAAGGAAATCTTTAAATACCTTAACACATTGTTACTAAATGAATTGGTAAGTAGAAGTGTTTGTATATTACCTGAAAATTTAGGTAAATTAAGGATTACTATTAGAAATGCACGTTATCAGAAGGATATGCAAACAGGTGAGATTAGACATATCCCACCAAAACCATTAGTGCGCTACAGTCCAAGCAAAACGATCAAAGAAACTGCAGCTAAAGTGCGTTGA", "aa": "MEKTSNTSKPLSRSEINKIIAVATGIKEKKIKEIFKYLNTLLLNELVSRSVCILPENLGKLRITIRNARYQKDMQTGEIRHIPPKPLVRYSPSKTIKETAAKVR", "aa_hexdigest": "2f0e07af14c4a3b6afd04bd9a12ba70a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47595", "query_cov": 1.0, "subject_cov": 0.9541284403669725, "identity": 1.0, "score": 193.0, "evalue": 2.41e-62, "valid": true, "gene": "HupA", "product": "Uncharacterized protein MG353 homolog", "uniref50_id": "UniRef50_P47595", "cog_id": "COG0776", "cog_category": "B", "go_ids": [ "GO:0003677", "GO:0030527" ] }, "pscc": { "uniref50_id": "UniRef50_P47595", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47595" ], "product": "Uncharacterized protein MG353" }, "genes": [ "hupA" ], "id": "JPCNHPFPBM_821", "locus": "GCA000292505_00820" }, { "type": "cds", "sequence": "contig_1", "start": 450690, "stop": 451103, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MG354", "db_xrefs": [ "RefSeq:WP_009885812.1", "SO:0001217", "UniParc:UPI000013968F", "UniRef:UniRef100_P47596", "UniRef:UniRef50_P47596", "UniRef:UniRef90_P47596" ], "nt": "ATGGAACAAAACAATATTAAAGAACAACTTATTTCTTTCTTTAATCAAGCATGTTCTACCCACCAAGAAAGACTTGATTTTATCTGTTCTACAAGAGAAAGTGATACTTTTTCTAGTGTTGATGTACCACTTGAACCCATTAAAAATATTATTGAAATAACTAAAGATGAAAATCAACAAATTGAAATTACAAAAATAGCTGTTAATAACATTAAAACATTATCTTCTGTTGGTGCAACCGGTCAGTATATGGCATCTTTCTTTTCAACAAATAGTGAGCCAGCTATAATATTTTGCGTCATTTATTTTTTATATCACTTTGGTTTTTTAAAAGATAACAATAAAAAACAAATAATAAAAAAGGCTTATGAAACTATTGCTGATAACATTGCTGACTATTTAAATGAAAATTAG", "aa": "MEQNNIKEQLISFFNQACSTHQERLDFICSTRESDTFSSVDVPLEPIKNIIEITKDENQQIEITKIAVNNIKTLSSVGATGQYMASFFSTNSEPAIIFCVIYFLYHFGFLKDNNKKQIIKKAYETIADNIADYLNEN", "aa_hexdigest": "7aae0a269f2241d48b8b839d81d6664a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013968F", "ncbi_nrp_id": "WP_009885812.1", "uniref100_id": "UniRef100_P47596", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013968F", "RefSeq:WP_009885812.1", "UniRef:UniRef100_P47596" ] }, "ips": { "uniref100_id": "UniRef100_P47596", "uniref90_id": "UniRef90_P47596" }, "psc": { "uniref90_id": "UniRef90_P47596", "product": "Uncharacterized protein MG354", "uniref50_id": "UniRef50_P47596" }, "pscc": { "uniref50_id": "UniRef50_P47596", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47596" ], "product": "Uncharacterized protein MG354" }, "genes": [], "id": "JPCNHPFPBM_822", "locus": "GCA000292505_00821" }, { "type": "cds", "sequence": "contig_1", "start": 451106, "stop": 451990, "strand": "-", "frame": 3, "gene": "clpB", "product": "Chaperone protein ClpB", "db_xrefs": [ "COG:COG0542", "COG:O", "GO:0005524", "GO:0005737", "GO:0016887", "GO:0034605", "SO:0001217", "UniRef:UniRef50_P47597", "UniRef:UniRef90_P47597" ], "nt": "TTGCACTTAGGTGATGAAATCAAAAAAAGAGTTAAAGGACAAGATGAAGCCATCGATGCTGTTGTTAACACTGTAATTAGAGGTAGAGTAAATATAAATGACCCAAACAAACCTATTGGTTCTTTCATCTTTTTAGGTTCTACTGGTGTTGGTAAAACTGAACTTGCCAAATCATTAGCAGAAGTTCTTTTTGACAATGAAAAAGCTCTGATTCGTTTTGATATGAGTGAATATATGGAAAAACATTCAGTAGCTAAATTAATTGGTGCACCCCCAGGGTACATAGGTTATGAACAATCAGGTTTGCTAACTGAAGCGGTTAGAAGAAAACCTTATAGCGTCTTGTTATTTGATGAAATTGAAAAGGCACATCCTGATGTAACTAATGTTTTATTACAAGTTTTAGATGATGGTACTTTAAAAGATTCACAAGGAAGGGTTGTTAATTTCAAAAATACTTTGATAATTATGACTTCTAACCTAGGTTCAAATTTTCTTTTAGAAGGAAAAAAAGATTTGGCCATTCAAAGTCTAAAGAAACATTTCCGTCCTGAATTTATAAATCGTATTGATGAGATAGTATTTTTCAATGTTCTTGAGAAAGATACAGTTTTATCGATAATCAACAGCTTGTTGGCACAACTTTCAAAACGCCTGAATAAACAAAATTTATTTTTTAATTTTGATTCAAATCTAACAGAGTTTATCTATAAAAGTAGTTTTGATCAACAGTTTGGTGCAAGACCAATTAAGCGCTTTATTGATCATAGTGTTGCAACTTTAATAGCTAAATATATCCTTCAGGGAAAGATAAAAAAAGGTGTTGGATACAACATTGCAGTTGTTAAAGACAATATTACCATTACACAAAATAATAAGTCTTAA", "aa": "MHLGDEIKKRVKGQDEAIDAVVNTVIRGRVNINDPNKPIGSFIFLGSTGVGKTELAKSLAEVLFDNEKALIRFDMSEYMEKHSVAKLIGAPPGYIGYEQSGLLTEAVRRKPYSVLLFDEIEKAHPDVTNVLLQVLDDGTLKDSQGRVVNFKNTLIIMTSNLGSNFLLEGKKDLAIQSLKKHFRPEFINRIDEIVFFNVLEKDTVLSIINSLLAQLSKRLNKQNLFFNFDSNLTEFIYKSSFDQQFGARPIKRFIDHSVATLIAKYILQGKIKKGVGYNIAVVKDNITITQNNKS", "aa_hexdigest": "2769379323d5850f6787d64dc1c2fdfb", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00265DA75C", "query_cov": 0.9659863945578231, "subject_cov": 0.8279883381924198, "identity": 0.5770000000000001, "score": 323.0, "evalue": 3.73e-107, "valid": false, "product": "AAA family ATPase", "uniref50_id": "UniRef50_A0A661I2M2" }, "genes": [ "clpB" ], "truncated": "3-prime", "pseudogene": { "start": 451106, "stop": 452290, "observations": { "mutation": [ 452287 ], "stop": [ 452287 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47597", "query_cov": 1.0, "subject_cov": 0.4117647058823529, "identity": 0.997, "score": 569.0, "evalue": 1.96e-198, "gene_start": 1, "gene_end": 294, "reference_start": 421, "reference_end": 714, "reference_sequence": "MNINFTPAGENRNFLQEIGRNINDEVLKNKVDPIIGRDNEIRRLIEILSRKSKNNPVLIGEPGVGKTAIVEGFVRRVVSNDVPLNLRDVEIYELSLSGLIAGTKFQGEFEKRINTILKQVKESNGRIILFIDEIHQIVGLGRNSSSGAMDIANILKPMLARGEIKVIGATTLKEYREYIEKDGALERRFQKILINEPSSQEALTIMRGLKTRWELFHNITIFDSALVAAVEMSTRYINERNLPDKAIDLIDEAAAKIKTEMSSEPVAIDSLKREIINLETEYAALKQDKENDNKQSKKEYLEKLKKQLDALKQKRDSLINEWKKEKADFENINKLKKEIEEFQTKLETYQSEGNYESASKILYSDIPRLKKELESAQQKYATSKHDLFKTEVSENEIAEVISQTTGIPLKKLLESEKDKLLHLGDEIKKRVKGQDEAIDAVVNTVIRGRVNINDPNKPIGSFIFLGSTGVGKTELAKSLAEVLFDNEKALIRFDMSEYMEKHSVAKLIGAPPGYIGYEQSGLLTEAVRRKPYSVLLFDEIEKAHPDVTNVLLQVLDDGTLKDSQGRVVNFKNTLIIMTSNLGSNFLLEGKKDLAIQSLKKHFRPEFINRIDEIVFFNVLEKDTVLSIINSLLAQLSKRLNKQNLFFNFDSNLTEFIYKSSFDQQFGARPIKRFIDHSVATLIAKYILQGKIKKGVGYNIAVVKDNITITQNNKS" }, "paralog": false, "identity": 0.9974619289340102, "score": 738.0, "evalue": 1.73e-267, "description": "Internal stop codon at 452287. Nonsense mutation around 452287.", "psc": { "uniref90_id": "UniRef90_P47597", "gene": "clpB", "product": "Chaperone protein ClpB", "uniref50_id": "UniRef50_P47597", "cog_id": "COG0542", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0016887", "GO:0034605" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47597", "UniRef:UniRef50_P47597", "COG:COG0542", "COG:O", "GO:0005737", "GO:0005524", "GO:0016887", "GO:0034605" ] }, "pscc": { "uniref50_id": "UniRef50_P47597", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47597" ], "product": "Chaperone protein ClpB" } }, "id": "JPCNHPFPBM_823", "locus": "GCA000292505_00822" }, { "type": "cds", "sequence": "contig_1", "start": 452285, "stop": 452557, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17871.7" ], "nt": "ATGTCAACTCGTTATATTAATGAACGTAATTTACCTGATAAAGCCATTGATCTTATTGATGAGGCTGCTGCTAAGATCAAAACAGAAATGTCATCTGAACCAGTTGCAATAGATAGTCTTAAACGTGAAATAATCAATCTTGAGACAGAGTATGCAGCTCTTAAACAAGATAAGGAAAATGATAACAAACAATCAAAGAAAGAATATTTAGAGAAACTAAAAAAACAATTAGATGCTCTTAAACAAAAGCGTGATTCACTTATAAATGAATGA", "aa": "MSTRYINERNLPDKAIDLIDEAAAKIKTEMSSEPVAIDSLKREIINLETEYAALKQDKENDNKQSKKEYLEKLKKQLDALKQKRDSLINE", "aa_hexdigest": "c2923b5f1575f1c68caf0e08cd475f95", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17871.7", "name": "AAA_lid_9", "length": 67, "aa_cov": 0.7444444444444445, "hmm_cov": 1.0, "evalue": 3.774608836172684e-20, "score": 66.9158706665039, "start": 1, "stop": 67 } ], "seq_stats": { "molecular_weight": 10451.764399999991, "isoelectric_point": 5.613271141052246 }, "id": "JPCNHPFPBM_824", "locus": "GCA000292505_00823" }, { "type": "cds", "sequence": "contig_1", "start": 452612, "stop": 453250, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00004.35", "PFAM:PF13191.12", "PFAM:PF01637.24", "PFAM:PF05621.17" ], "nt": "ATGAATATTAATTTCACACCTGCTGGTGAAAATCGTAATTTTTTGCAAGAAATTGGTCGTAATATTAACGATGAAGTATTAAAAAATAAGGTCGATCCTATTATTGGAAGAGATAACGAAATTCGTCGTTTAATTGAGATATTAAGTCGTAAAAGCAAAAACAATCCTGTTTTAATTGGTGAACCTGGAGTTGGTAAAACCGCAATAGTAGAAGGTTTTGTTAGAAGAGTTGTTAGTAATGATGTACCTTTAAATTTAAGGGATGTAGAAATTTATGAACTATCTCTTTCTGGATTAATTGCTGGCACTAAATTCCAAGGTGAATTTGAAAAAAGAATTAATACCATTCTTAAGCAAGTAAAAGAATCAAATGACAAGATTATTCTTTTTATTGATGAAATTCACCAAATAGTTGGATTAGGACGTAATTCTAGCAGTGGTGCAATGGATATTGCCAATATATTAAAGCCGATGCTAGCTCGAGGAGAAATAAAAGTAATTGGCGCTACTACTCTAAAAGAATACCGGGAATACATTGAAAAAGATGGCGCTTTAGAACGTAGATTTCAAAAAATTCTTATTAACGAGCCTAGTAGTCAAGAGGCACTAACAATTATGCGTGGTTTAAAAACACGTTGA", "aa": "MNINFTPAGENRNFLQEIGRNINDEVLKNKVDPIIGRDNEIRRLIEILSRKSKNNPVLIGEPGVGKTAIVEGFVRRVVSNDVPLNLRDVEIYELSLSGLIAGTKFQGEFEKRINTILKQVKESNDKIILFIDEIHQIVGLGRNSSSGAMDIANILKPMLARGEIKVIGATTLKEYREYIEKDGALERRFQKILINEPSSQEALTIMRGLKTR", "aa_hexdigest": "fb6564c408ce3887d25494e3fde1010b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001C4E8114", "query_cov": 0.8584905660377359, "subject_cov": 0.9479166666666666, "identity": 0.615, "score": 235.0, "evalue": 6.52e-76, "valid": false, "product": "AAA family ATPase", "uniref50_id": "UniRef50_J9G8B9" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00004.35", "name": "AAA", "length": 142, "aa_cov": 0.6367924528301887, "hmm_cov": 0.8732394366197183, "evalue": 2.4311605675210715e-18, "score": 61.95420455932617, "start": 57, "stop": 191 }, { "id": "PF13191.12", "name": "AAA_16", "length": 51, "aa_cov": 0.22641509433962265, "hmm_cov": 1.0, "evalue": 1.9214059480175674e-10, "score": 36.5750617980957, "start": 34, "stop": 81 }, { "id": "PF01637.24", "name": "ATPase_2", "length": 190, "aa_cov": 0.8066037735849056, "hmm_cov": 0.9947368421052631, "evalue": 5.278061719753526e-11, "score": 37.83116149902344, "start": 37, "stop": 207 }, { "id": "PF05621.17", "name": "TniB", "length": 168, "aa_cov": 0.7311320754716981, "hmm_cov": 0.9702380952380952, "evalue": 1.3092635347096525e-10, "score": 36.1097297668457, "start": 40, "stop": 194 } ], "seq_stats": { "molecular_weight": 23895.345800000014, "isoelectric_point": 9.268635749816895 }, "id": "JPCNHPFPBM_825", "locus": "GCA000292505_00824" }, { "type": "cds", "sequence": "contig_1", "start": 453887, "stop": 454246, "strand": "-", "frame": 3, "gene": "cotS", "product": "putative protein MG356", "db_xrefs": [ "COG:COG0510", "COG:H", "GO:0004305", "GO:0005737", "GO:0006646", "SO:0001217", "UniRef:UniRef50_Q49423", "UniRef:UniRef90_Q49423" ], "nt": "ATGGATTTAAAAAAAGCGGTTGCTTTTCTTTGTGAAAAACTTCAAGTTGTAGAAACAGATATTTTCAAGATAGAGCAAATTCATTCTGGTTTTACTAATTTTTCTTTTTTTTGTGAATTGAAAGATCATAGTAAATTTCAAATAAGACTTTCAAGAAAAGATGTTATTTTAGACAGAAGAAATGAACAAATTATTTTGCAACTAGTAGAACCTTTTTTTTCAAACCCATTTGTTTATTTTGATGTAACAACAGGTAATGCTATAAAAAGGTGGATAGAAGGCACTCAACCTAAAAGAGCAACTGATCTATTTTTATGCAGATTGGTTGAATCTTTAAAAAAAGTTCATTCAGTTGACTGA", "aa": "MDLKKAVAFLCEKLQVVETDIFKIEQIHSGFTNFSFFCELKDHSKFQIRLSRKDVILDRRNEQIILQLVEPFFSNPFVYFDVTTGNAIKRWIEGTQPKRATDLFLCRLVESLKKVHSVD", "aa_hexdigest": "94cdca7201e50f6974daa0173640bf07", "start_type": "ATG", "rbs_motif": null, "genes": [ "cotS" ], "truncated": "5-prime", "pseudogene": { "start": 453584, "stop": 454246, "observations": { "mutation": [ 453685, 453724, 453889 ], "stop": [ 453685, 453724, 453889 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49423", "query_cov": 1.0, "subject_cov": 0.425, "identity": 1.0, "score": 242.0, "evalue": 7.53e-79, "gene_start": 1, "gene_end": 119, "reference_start": 1, "reference_end": 119, "reference_sequence": "MDLKKAVAFLCEKLQVVETDIFKIEQIHSGFTNFSFFCELKDHSKFQIRLSRKDVILDRRNEQIILQLVEPFFSNPFVYFDVTTGNAIKRWIEGTQPKRATDLFLCRLVESLKKVHSVDWKPFANELLIFDPLVYFEKTKLSAFYKRLYLNLTKKHIDIPKTLCHHDATFDNLVWTPKKQVILIDFEWSCVDNPYYEIANIIREELTIETANKLIDIYGGLKRKLVFETVIYVLLFAFQWTEIMPQTEAILNYRKWVEKRLEYFLKALFPSIWKQAQKNS" }, "paralog": false, "identity": 0.9863636363636363, "score": 435.0, "evalue": 1.68e-157, "description": "Internal stop codon at 453685, 453724, 453889. Nonsense mutation around 453685, 453724, 453889.", "psc": { "uniref90_id": "UniRef90_Q49423", "gene": "CotS", "product": "Uncharacterized protein MG356", "uniref50_id": "UniRef50_Q49423", "cog_id": "COG0510", "cog_category": "H", "go_ids": [ "GO:0005737", "GO:0004305", "GO:0006646" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49423", "UniRef:UniRef50_Q49423", "COG:COG0510", "COG:H", "GO:0005737", "GO:0004305", "GO:0006646" ] }, "pscc": { "uniref50_id": "UniRef50_Q49423", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49423" ], "product": "Uncharacterized protein MG356" } }, "id": "JPCNHPFPBM_826", "locus": "GCA000292505_00825" }, { "type": "cds", "sequence": "contig_1", "start": 454259, "stop": 454771, "strand": "-", "frame": 3, "gene": "ackA", "product": "Acetate kinase", "db_xrefs": [ "COG:C", "COG:COG0282", "EC:2.7.2.1", "GO:0000287", "GO:0005524", "GO:0005737", "GO:0006083", "GO:0006085", "GO:0008776", "KEGG:K00925", "SO:0001217", "UniRef:UniRef50_P75245", "UniRef:UniRef90_P47599" ], "nt": "ATGGGATTCACTCCCCTTGAAGGATTAATAATGGGAACACGTAGTGGTGATATTGATCCTGCCATTGTTAGTTACATTGCTGAACAGCAAAAGCTTTCATGTAATGATGTTGTAAATGAATTAAATAAAAAGAGTGGAATGTTTGCTATAACAGGTAGTTCTGACATGCGTGATATTTTTGATAAACCAGAAATTAATGATATTGCTATAAAAATGTATGTTAATCGTGTTGCTGACTATATTGCTAAATACCTAAATCAACTTTCAGGTGAAATTGATAGCTTGGTATTTACTGGGGGAGTTGGTGAAAATGCTAGTTATTGTGTGCAATTAATAATTGAAAAAGTTGCTTCACTTGGTTTTAAAACTAACAGTAATTTATTTGGAAATTATCAAGATAGTTCTCTAATTTCAACAAATGAAAGCAAGTATCAAATTTTTAGAGTTCGTACAAATGAGGAATTGATGATTGTAGAAGATGCTTTGAGAGTAAGTACAAACATTAAAAAATAA", "aa": "MGFTPLEGLIMGTRSGDIDPAIVSYIAEQQKLSCNDVVNELNKKSGMFAITGSSDMRDIFDKPEINDIAIKMYVNRVADYIAKYLNQLSGEIDSLVFTGGVGENASYCVQLIIEKVASLGFKTNSNLFGNYQDSSLISTNESKYQIFRVRTNEELMIVEDALRVSTNIKK", "aa_hexdigest": "7ec6ac10e44b468ab21afd7c5c0886d4", "start_type": "ATG", "rbs_motif": null, "genes": [ "ackA" ], "truncated": "3-prime", "pseudogene": { "start": 454259, "stop": 455071, "observations": { "mutation": [ 454945 ], "stop": [ 454945 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47599", "query_cov": 1.0, "subject_cov": 0.43256997455470736, "identity": 1.0, "score": 332.0, "evalue": 6.89e-112, "gene_start": 1, "gene_end": 170, "reference_start": 224, "reference_end": 393, "reference_sequence": "MQSHKILVVNAGSSSIKFQLFNDKKQVLAKGLCERIFIDGFFKLEFNQKKIEEKVQFNDHNLAVKHFLNALKKNKIITELSEIGLIGHRVVQGANYFTDAVLVDTHSLAKIKEFIKLAPLHNKPEADVIEIFLKEIKTAKNVAVFDTTFHTTIPRENYLYAVPENWEKNNLVRRYGFHGTSYKYINEFLEKKFNKKPLNLIVCHLGNGASVCAIKQGKSLNTSMGFTPLEGLIMGTRSGDIDPAIVSYIAEQQKLSCNDVVNELNKKSGMFAITGSSDMRDIFDKPEINDIAIKMYVNRVADYIAKYLNQLSGEIDSLVFTGGVGENASYCVQLIIEKVASLGFKTNSNLFGNYQDSSLISTNESKYQIFRVRTNEELMIVEDALRVSTNIKK" }, "paralog": false, "identity": 0.9962962962962963, "score": 532.0, "evalue": 3.21e-193, "description": "Internal stop codon at 454945. Nonsense mutation around 454945.", "psc": { "uniref90_id": "UniRef90_P47599", "gene": "ackA", "product": "Acetate kinase", "ec_ids": [ "2.7.2.1" ], "uniref50_id": "UniRef50_P75245", "cog_id": "COG0282", "cog_category": "C", "kegg_orthology_id": "K00925", "go_ids": [ "GO:0005737", "GO:0008776", "GO:0005524", "GO:0000287", "GO:0006083", "GO:0006085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47599", "EC:2.7.2.1", "UniRef:UniRef50_P75245", "COG:COG0282", "COG:C", "KEGG:K00925", "GO:0005737", "GO:0008776", "GO:0005524", "GO:0000287", "GO:0006083", "GO:0006085" ] }, "pscc": { "uniref50_id": "UniRef50_P75245", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75245" ], "product": "Acetate kinase" } }, "id": "JPCNHPFPBM_827", "locus": "GCA000292505_00826" }, { "type": "cds", "sequence": "contig_1", "start": 454943, "stop": 455440, "strand": "-", "frame": 3, "gene": "ackA", "product": "Acetate kinase", "db_xrefs": [ "COG:C", "COG:COG0282", "EC:2.7.2.1", "GO:0000287", "GO:0005524", "GO:0005737", "GO:0006083", "GO:0006085", "GO:0008776", "KEGG:K00925", "SO:0001217", "UniRef:UniRef50_P75245", "UniRef:UniRef90_P47599" ], "nt": "ATGCAAAGTCACAAAATCTTGGTTGTTAATGCAGGTAGCAGTTCAATTAAATTTCAACTTTTTAATGATAAAAAACAAGTACTAGCTAAAGGACTTTGTGAACGTATTTTCATTGATGGTTTTTTTAAGCTTGAATTTAATCAAAAAAAGATAGAAGAAAAGGTTCAATTTAATGATCATAATCTTGCTGTTAAGCATTTTTTAAATGCGCTTAAAAAAAACAAAATTATTACTGAACTTTCAGAAATTGGGCTAATAGGGCATAGAGTAGTACAAGGAGCAAATTATTTTACAGATGCAGTTCTTGTTGATACACATTCACTAGCAAAAATAAAAGAATTCATTAAGTTAGCACCGCTTCATAATAAACCAGAAGCAGATGTTATTGAAATTTTTCTAAAAGAGATAAAAACTGCTAAGAATGTTGCTGTATTTGATACCACTTTTCACACTACTATTCCAAGGGAAAATTATCTTTATGCAGTTCCTGAAAATTGA", "aa": "MQSHKILVVNAGSSSIKFQLFNDKKQVLAKGLCERIFIDGFFKLEFNQKKIEEKVQFNDHNLAVKHFLNALKKNKIITELSEIGLIGHRVVQGANYFTDAVLVDTHSLAKIKEFIKLAPLHNKPEADVIEIFLKEIKTAKNVAVFDTTFHTTIPRENYLYAVPEN", "aa_hexdigest": "74bbfad2789e566929303c95e3086fd4", "start_type": "ATG", "rbs_motif": null, "genes": [ "ackA" ], "truncated": "5-prime", "pseudogene": { "start": 454640, "stop": 455440, "observations": { "mutation": [ 454945 ], "stop": [ 454945 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47599", "query_cov": 1.0, "subject_cov": 0.4198473282442748, "identity": 1.0, "score": 328.0, "evalue": 2.62e-110, "gene_start": 1, "gene_end": 165, "reference_start": 1, "reference_end": 165, "reference_sequence": "MQSHKILVVNAGSSSIKFQLFNDKKQVLAKGLCERIFIDGFFKLEFNQKKIEEKVQFNDHNLAVKHFLNALKKNKIITELSEIGLIGHRVVQGANYFTDAVLVDTHSLAKIKEFIKLAPLHNKPEADVIEIFLKEIKTAKNVAVFDTTFHTTIPRENYLYAVPENWEKNNLVRRYGFHGTSYKYINEFLEKKFNKKPLNLIVCHLGNGASVCAIKQGKSLNTSMGFTPLEGLIMGTRSGDIDPAIVSYIAEQQKLSCNDVVNELNKKSGMFAITGSSDMRDIFDKPEINDIAIKMYVNRVADYIAKYLNQLSGEIDSLVFTGGVGENASYCVQLIIEKVASLGFKTNSNLFGNYQDSSLISTNESKYQIFRVRTNEELMIVEDALRVSTNIKK" }, "paralog": false, "identity": 0.9962406015037594, "score": 528.0, "evalue": 1.25e-191, "description": "Internal stop codon at 454945. Nonsense mutation around 454945.", "psc": { "uniref90_id": "UniRef90_P47599", "gene": "ackA", "product": "Acetate kinase", "ec_ids": [ "2.7.2.1" ], "uniref50_id": "UniRef50_P75245", "cog_id": "COG0282", "cog_category": "C", "kegg_orthology_id": "K00925", "go_ids": [ "GO:0005737", "GO:0008776", "GO:0005524", "GO:0000287", "GO:0006083", "GO:0006085" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47599", "EC:2.7.2.1", "UniRef:UniRef50_P75245", "COG:COG0282", "COG:C", "KEGG:K00925", "GO:0005737", "GO:0008776", "GO:0005524", "GO:0000287", "GO:0006083", "GO:0006085" ] }, "pscc": { "uniref50_id": "UniRef50_P75245", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75245" ], "product": "Acetate kinase" } }, "id": "JPCNHPFPBM_828", "locus": "GCA000292505_00827" }, { "type": "cds", "sequence": "contig_1", "start": 455547, "stop": 455756, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_014894048.1", "SO:0001217", "UniParc:UPI00027B3165", "UniRef:UniRef100_UPI00027B3165", "UniRef:UniRef50_A0AAN4XFQ7", "UniRef:UniRef90_UPI00027B3165" ], "nt": "ATGAAAATTAAAAAATGCTATATTATCGCTGTTATTTCAGCTTTTATTGGTTTTCTTTTTTACTTTTTATCTTCACTTATAGGGATAACAGATGTTAATAATGCAAAAACAGTAGCAACTGTATTTTTTGTATTAGCAACATTAATAGTTGTTAATGCTATTGTCTTCTGGATTCTTTATCTATTCAAAAAAAAGAACGTAGTTAAGTAA", "aa": "MKIKKCYIIAVISAFIGFLFYFLSSLIGITDVNNAKTVATVFFVLATLIVVNAIVFWILYLFKKKNVVK", "aa_hexdigest": "2aec87b6506e412f4fc76993107521a6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3165", "ncbi_nrp_id": "WP_014894048.1", "uniref100_id": "UniRef100_UPI00027B3165", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3165", "RefSeq:WP_014894048.1", "UniRef:UniRef100_UPI00027B3165" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3165", "uniref90_id": "UniRef90_UPI00027B3165" }, "psc": { "uniref90_id": "UniRef90_UPI00027B3165", "uniref50_id": "UniRef50_A0AAN4XFQ7" }, "pscc": { "uniref50_id": "UniRef50_A0AAN4XFQ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAN4XFQ7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7823.556999999997, "isoelectric_point": 9.937430763244631 }, "id": "JPCNHPFPBM_829", "locus": "GCA000292505_00828" }, { "type": "cds", "sequence": "contig_1", "start": 455819, "stop": 456046, "strand": "+", "frame": 2, "gene": null, "product": "MPN534 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75244", "UniRef:UniRef90_UPI0001B417BC" ], "nt": "ATGGAAACTATCAGAGAAACACTTTCTTCTATACAAAGAAGCAATTTTTTATTAAATGAAAGCGCACAAATTCTCAATACGTGTGTAAACAAGAACACATTGCTTGACGATAAGCTGCGCAATTCGATTAATTTTGCACTTAGCATCATTAAGCTAGCAGACTTTTGTCTAAAGGATGATACTGAAAAAATTAATCAATTCTTAGGTAAAACATATGATTTACCATGA", "aa": "METIRETLSSIQRSNFLLNESAQILNTCVNKNTLLDDKLRNSINFALSIIKLADFCLKDDTEKINQFLGKTYDLP", "aa_hexdigest": "db9c1096daeeb392e83726590a16739b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 455819, "stop": 456244, "observations": { "mutation": [ 456044 ], "stop": [ 456044 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0001B417BC", "query_cov": 1.0, "subject_cov": 0.5319148936170213, "identity": 1.0, "score": 148.0, "evalue": 1.92e-44, "gene_start": 1, "gene_end": 75, "reference_start": 1, "reference_end": 75, "reference_sequence": "METIRETLSSIQRSNFLLNESAQILNTCVNKNTLLDDKLRNSINFALSIIKLADFCLKDDTEKINQFLGKTYDLPWEFQFNQKDKHIECTVKDNFGREKLINLIFQIGDAIETYHTTLIRFKIPKHCLNARDQIKKIMEGK" }, "paralog": false, "identity": 0.9929078014184397, "score": 281.0, "evalue": 1.71e-99, "description": "Internal stop codon at 456044. Nonsense mutation around 456044.", "psc": { "uniref90_id": "UniRef90_UPI0001B417BC", "product": "MPN534 family protein", "uniref50_id": "UniRef50_P75244", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0001B417BC", "UniRef:UniRef50_P75244" ] }, "pscc": { "uniref50_id": "UniRef50_P75244", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75244" ], "product": "Uncharacterized protein MPN_534" } }, "id": "JPCNHPFPBM_830", "locus": "GCA000292505_00829" }, { "type": "cds", "sequence": "contig_1", "start": 456364, "stop": 456864, "strand": "+", "frame": 1, "gene": "ruvA", "product": "Holliday junction branch migration protein RuvA", "db_xrefs": [ "COG:COG0632", "COG:L", "EC:3.6.4.12", "GO:0000400", "GO:0003678", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379", "GO:0036121", "GO:0048476", "GO:0061749", "GO:1990518", "SO:0001217", "UniRef:UniRef50_Q49424", "UniRef:UniRef90_Q49424" ], "nt": "TTGGAAAAAAATAAGCAAATTTTTTGTCATATTATTAAAAAAATTGTCGCTAACCAAATTATAGAAGAGGCTTTTGCCTTTAATACTCTAGAAGAAAAAGAGTGGTTCTGTAGATTAATAGAACTCAATGGTATTGGTAGTAAAACTGCACTTAATTTGCTCAATAATGACCTTGAGGAAATTAAACAATACATTCTGGAAAATAACTACAGTGCATTATGTGGTATTAACGGTGTAAATAACAAAATAGCTCGTGCACTTTTATCACTTGAAATATTTGAAAAATCTGAAAATAATAAAAATATTAAAGGAGTTCAAGTTGCTGATGGTTATGATGAATTGTTTGAAACACTAAAGTCACTTGGTTACAAACAACAAGAAATTCAGGATGCACTAAAAATGATAGAAGTAAAACCTGATTTTGATATAAGTCAGTTAGTTGCAGAAGTAATTAAATTAATGTCTTTTAAGAATAATGAAATTACAAATAAAACCGCCTAA", "aa": "MEKNKQIFCHIIKKIVANQIIEEAFAFNTLEEKEWFCRLIELNGIGSKTALNLLNNDLEEIKQYILENNYSALCGINGVNNKIARALLSLEIFEKSENNKNIKGVQVADGYDELFETLKSLGYKQQEIQDALKMIEVKPDFDISQLVAEVIKLMSFKNNEITNKTA", "aa_hexdigest": "914cde5cfa7e1f2a78d3a2c8ce195e3d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q49424", "query_cov": 1.0, "subject_cov": 0.8097560975609757, "identity": 0.9940000000000001, "score": 323.0, "evalue": 4.61e-111, "valid": true, "gene": "ruvA", "product": "Holliday junction branch migration protein RuvA", "ec_ids": [ "3.6.4.12" ], "uniref50_id": "UniRef50_Q49424", "cog_id": "COG0632", "cog_category": "L", "go_ids": [ "GO:0000400", "GO:0003678", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379", "GO:0036121", "GO:0048476", "GO:0061749", "GO:1990518" ] }, "pscc": { "uniref50_id": "UniRef50_Q49424", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49424" ], "product": "Holliday junction branch migration complex subunit RuvA" }, "genes": [ "ruvA" ], "id": "JPCNHPFPBM_831", "locus": "GCA000292505_00830" }, { "type": "cds", "sequence": "contig_1", "start": 456839, "stop": 457762, "strand": "+", "frame": 2, "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "db_xrefs": [ "COG:COG2255", "COG:L", "EC:3.6.4.-", "EC:3.6.4.12", "GO:0000400", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0016887", "GO:0036121", "GO:0048476", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990518", "RefSeq:WP_014894679.1", "SO:0001217", "UniParc:UPI00027B3E4A", "UniRef:UniRef100_UPI00027B3E4A", "UniRef:UniRef50_Q49425", "UniRef:UniRef90_Q49425" ], "nt": "ATGAAATTACAAATAAAACCGCCTAATACCTTTGATGAATTTGTAGGAAAACAAGAAATAATTAGTCAAATTCAATTAAGTATTAAAGCATCTAAATTAAATAAAACACAACTAGATCATATCTTGTTATATGGCCCACCTGGTGTGGGTAAAACTACTTTAGCCAGATTAATAGCAAATGAATTGAAAACAAAGTTGCAAATTATTCAAGGTGGACATTTACAAAAACCAAGCGATTTCTTAAACGCAATTTCACTCATTAAAAAAGGTGATGTTCTTTTTATAGATGAGATCCATGCCGTAGCACCTAATGTCATGGAACTAATGTATCCAGTTATGGATGTGTTCAAAATACAAGTATTAATTGGCAAGGATTTTAATTCCAAGATAGTTGAAATGAAGGTAAATCCTTTTACTCTAATTGGTGCAACTACACAACTTGGTAAAATCATCAATCCTTTAGAAGATAGATTTGGCGTTATCTTAAACATTAACTATTATTCAAATGCTGAAATTGAAAAGATGGTAAGTATCTATGGAAAGCAAATGAAGTTAGAGCTAAATTCAAATGAAATTTCAGCTATCACTGAACATAGTAAACAAACACCAAGAATTGCAATTAGAATAGTTAGAAGAATATTTGAACAAAAAATTGTTAATAAAAAAATAGACCTTGAGAGTTTGTTTAAGAATTTAATGATTTATAAAAATGGTCTGCAAAGTATTGATGTCCAATATCTTGAGGTTTTAAATCGCCAAAATGAACCACAAGGAATTAAGTCAATTAGTTCCATGTTAGGTATAGACAGACACACTATAGAAAATAAAATTGAACCTTTTTTGTTGCGTGAAAATATGATTCAAAAAACCAAAAAAGGCAGGATTATTACAAATAGCGGAAGAGAATATTTAGTTAACTTTTAA", "aa": "MKLQIKPPNTFDEFVGKQEIISQIQLSIKASKLNKTQLDHILLYGPPGVGKTTLARLIANELKTKLQIIQGGHLQKPSDFLNAISLIKKGDVLFIDEIHAVAPNVMELMYPVMDVFKIQVLIGKDFNSKIVEMKVNPFTLIGATTQLGKIINPLEDRFGVILNINYYSNAEIEKMVSIYGKQMKLELNSNEISAITEHSKQTPRIAIRIVRRIFEQKIVNKKIDLESLFKNLMIYKNGLQSIDVQYLEVLNRQNEPQGIKSISSMLGIDRHTIENKIEPFLLRENMIQKTKKGRIITNSGREYLVNF", "aa_hexdigest": "406ed9a7b3ff0e72a00195e035b626b9", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3E4A", "ncbi_nrp_id": "WP_014894679.1", "uniref100_id": "UniRef100_UPI00027B3E4A", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3E4A", "RefSeq:WP_014894679.1", "UniRef:UniRef100_UPI00027B3E4A" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3E4A", "uniref90_id": "UniRef90_Q49425" }, "psc": { "uniref90_id": "UniRef90_Q49425", "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "ec_ids": [ "3.6.4.-", "3.6.4.12" ], "uniref50_id": "UniRef50_Q49425", "cog_id": "COG2255", "cog_category": "L", "go_ids": [ "GO:0000400", "GO:0003677", "GO:0003689", "GO:0005524", "GO:0005737", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0016887", "GO:0036121", "GO:0048476", "GO:0061749", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "GO:1990518" ] }, "pscc": { "uniref50_id": "UniRef50_Q49425", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49425" ], "product": "Holliday junction branch migration complex subunit RuvB" }, "genes": [ "ruvB" ], "id": "JPCNHPFPBM_832", "locus": "GCA000292505_00831" }, { "type": "cds", "sequence": "contig_1", "start": 457752, "stop": 458039, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATATTCAAACGATTATAGAAAACTACTGTCAATAACAAAACGATTATTCAACAGACTATTAATTAATACTGAAAAGAACGTTCGATTAATAGGTATTAGTTTTTTTGATTTAAAAAAGATAGATACAGATGAAGGACAGAAAAAATCTTTATTTTATCAATTCATACCAAAATCAATTAGCAAATTAAGCGAAGAATCTTCACTTGATAAATTAATTTTTGATATTAATGAAAGTTTTGGTTTTGAAATTATTAAAAGAGCTAATAAGCTTAAAAGTTAA", "aa": "MKKYSNDYRKLLSITKRLFNRLLINTEKNVRLIGISFFDLKKIDTDEGQKKSLFYQFIPKSISKLSEESSLDKLIFDINESFGFEIIKRANKLKS", "aa_hexdigest": "187c5e3628d36d6c5a42578e9c0f0fd3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11227.981499999989, "isoelectric_point": 9.800693321228025 }, "id": "JPCNHPFPBM_833", "locus": "GCA000292505_00832" }, { "type": "cds", "sequence": "contig_1", "start": 458424, "stop": 458987, "strand": "-", "frame": 2, "gene": "dinP", "product": "putative protein MG360", "db_xrefs": [ "COG:COG0389", "COG:L", "GO:0003684", "GO:0003887", "GO:0009432", "GO:0042276", "SO:0001217", "UniRef:UniRef50_P75241", "UniRef:UniRef90_Q49426" ], "nt": "ATGATTAACACCTTTACATATTTTGAACCAGAATATTTAATTGATAAAAATTTAATTTTTCTTTATTTTGATTTTGATGCTTTTTTTGCATCTGTTGAAGAATTAGAAAATCCAGAATTAGTTAATCAACCCTTAATAGTTGGTAATCGCTTTTCAAGAAGTGTAGTTTCAACTTGTAACTATGTTGCTCGTAGTTATGGAATTAGATCTGGAATGTCTATTTTAAAAGCATTAGAACTATGTCCAAATGCTATTTTTGCACATTCTAATTTTCGTAATTACAGAAAACATTCTAAACGTATTTTTTCAGTTATTGAAAGCACTTTTTCATTAAAAATTGATGTTCTTTCTGTTGATGAAGGGGTTGCTTGTTTTCAAAACATTTCATTTAAAAAGGCATTTTTAATAGCGAAAAAAATAAAAAATTTTGTTTTTCAAAACTTGAGAATCAAAATTTCAATTGGCATCTCAGATCATTTTTTAATTGCTAAGATTTTTTCAAATCAAGCAAAACCATTTGGAATTAAAAGTTGTTCAGTTAAGGATATTAAAAAAAAACTTTGA", "aa": "MINTFTYFEPEYLIDKNLIFLYFDFDAFFASVEELENPELVNQPLIVGNRFSRSVVSTCNYVARSYGIRSGMSILKALELCPNAIFAHSNFRNYRKHSKRIFSVIESTFSLKIDVLSVDEGVACFQNISFKKAFLIAKKIKNFVFQNLRIKISIGISDHFLIAKIFSNQAKPFGIKSCSVKDIKKKL", "aa_hexdigest": "d6153e2b814a3478ebb588cb1d487661", "start_type": "ATG", "rbs_motif": null, "genes": [ "dinP" ], "truncated": "5-prime", "pseudogene": { "start": 458121, "stop": 458987, "observations": { "mutation": [ 458255, 458288, 458426 ], "stop": [ 458255, 458288, 458426 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49426", "query_cov": 1.0, "subject_cov": 0.45498783454987834, "identity": 1.0, "score": 367.0, "evalue": 5.43e-125, "gene_start": 1, "gene_end": 187, "reference_start": 1, "reference_end": 187, "reference_sequence": "MINTFTYFEPEYLIDKNLIFLYFDFDAFFASVEELENPELVNQPLIVGNRFSRSVVSTCNYVARSYGIRSGMSILKALELCPNAIFAHSNFRNYRKHSKRIFSVIESTFSLKIDVLSVDEGVACFQNISFKKAFLIAKKIKNFVFQNLRIKISIGISDHFLIAKIFSNQAKPFGIKSCSVKDIKKKLWPLPITEIPGIGEKHIDLVFKNNFYKINDLAVCEDASLLKKVFGNFWESLKAVSLGKWYTDNNNQVKSRSFAVSETLEDLNYSNNQLNKKLTQIFDQLFIRLQLSSQVCKGIVVQLKSNDFIVNSHSNKMKKYSNDYRKLLSITKRLFNRLLINTEKNVRLIGISFFDLKKIDTDEGQKKSLFYQFIPKSISKLSEESSLDKLIFDINESFGFEIIKRANKLKS" }, "paralog": false, "identity": 0.9895833333333334, "score": 558.0, "evalue": 5.39e-203, "description": "Internal stop codon at 458255, 458288, 458426. Nonsense mutation around 458255, 458288, 458426.", "psc": { "uniref90_id": "UniRef90_Q49426", "gene": "DinP", "product": "Uncharacterized protein MG360", "uniref50_id": "UniRef50_P75241", "cog_id": "COG0389", "cog_category": "L", "go_ids": [ "GO:0003684", "GO:0003887", "GO:0042276", "GO:0009432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49426", "UniRef:UniRef50_P75241", "COG:COG0389", "COG:L", "GO:0003684", "GO:0003887", "GO:0042276", "GO:0009432" ] }, "pscc": { "uniref50_id": "UniRef50_P75241", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75241" ], "product": "Uncharacterized protein MG360 homolog" } }, "id": "JPCNHPFPBM_834", "locus": "GCA000292505_00833" }, { "type": "cds", "sequence": "contig_1", "start": 459107, "stop": 459595, "strand": "+", "frame": 2, "gene": "rplJ", "product": "50S ribosomal protein L10", "db_xrefs": [ "COG:COG0244", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0022625", "GO:0070180", "KEGG:K02864", "RefSeq:WP_009885818.1", "SO:0001217", "UniParc:UPI0000133B39", "UniRef:UniRef100_P36263", "UniRef:UniRef50_P36263", "UniRef:UniRef90_P36263" ], "nt": "ATGGTAGATAGTAAGAAAAATAAAAAACAGCAGGTTACGGATTTTTCTAATTTACTCTCTCAAAGTAAAGGATTTGTTATTTTTGACTATTCAGGAATGTCTGCTGTTGATGCAACTTTAATGAGAAAAAAGTTGTTTAATAAGGGTAGTAAGATAAAAATTGTTAAAAACAATATCTTAAGACGTGCTTTAAAAACTAGTAATTTTGAAGGTGTTGATGAATCGGTCATCAAAGGAAAAATTGCAGTTGCTGTTGGTATTAACGAGATCTTAGAAACCTTAAAAGTTGTTGATAGTGTAGTTAAAGAAAAAGAGTTAATGAAATTTGTTTGTGGTCATTTTGATAATCGTATTTTTAATAGTGATGACTTACAAAAAATAGCAAAACTCCCTGGTAGAAATGAACTTTATGGAATGTTTCTTTCAGTTCTACAAGCACCATTACGAAAATTTCTCTATGCTCTTCAGGCAGTAAGGAATGCTAAGTAA", "aa": "MVDSKKNKKQQVTDFSNLLSQSKGFVIFDYSGMSAVDATLMRKKLFNKGSKIKIVKNNILRRALKTSNFEGVDESVIKGKIAVAVGINEILETLKVVDSVVKEKELMKFVCGHFDNRIFNSDDLQKIAKLPGRNELYGMFLSVLQAPLRKFLYALQAVRNAK", "aa_hexdigest": "4d14f11ca59c4d52f196969e98ce2e5e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133B39", "ncbi_nrp_id": "WP_009885818.1", "uniref100_id": "UniRef100_P36263", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133B39", "RefSeq:WP_009885818.1", "UniRef:UniRef100_P36263" ] }, "ips": { "uniref100_id": "UniRef100_P36263", "uniref90_id": "UniRef90_P36263" }, "psc": { "uniref90_id": "UniRef90_P36263", "gene": "rplJ", "product": "50S ribosomal protein L10", "uniref50_id": "UniRef50_P36263", "cog_id": "COG0244", "cog_category": "J", "kegg_orthology_id": "K02864", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0022625", "GO:0070180" ] }, "pscc": { "uniref50_id": "UniRef50_P36263", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P36263" ], "product": "Large ribosomal subunit protein uL10" }, "genes": [ "rplJ" ], "id": "JPCNHPFPBM_835", "locus": "GCA000292505_00834" }, { "type": "cds", "sequence": "contig_1", "start": 459618, "stop": 459986, "strand": "+", "frame": 3, "gene": "rplL", "product": "50S ribosomal protein L7/L12", "db_xrefs": [ "COG:COG0222", "COG:J", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "RefSeq:WP_009885819.1", "SO:0001217", "UniParc:UPI00001341B3", "UniRef:UniRef100_P36255", "UniRef:UniRef50_P36255", "UniRef:UniRef90_P36255" ], "nt": "ATGGGAAAACTAGATAAAAAACAATTAATTGAATCTCTAAAAGAGATGACTATAGTTGAAATTGATGAAATAATCAAGGCTGTTGAAGAAGCATTTGGTGTAACTGCAACTCCAATAGTAGCTGCTGGCGCAGCTGGTGCTACACAAGAAGCTGCTAGCGAAGTTAGTGTAAAGGTAACAGGATATGCTGATAATGCTAAGTTAGCTGTTTTAAAACTTTATCGTGAAATTACTGGAGTTGGTTTAATGGAAGCTAAAACTGCAGTTGAAAAATTACCTTGCGTTGTAAAACAAGATATTAAACCAGAAGAAGCAGAAGAACTTAAAAAGCGTTTTGTTGAAGTTGGTGCAACTGTTGAAGTTAAATAA", "aa": "MGKLDKKQLIESLKEMTIVEIDEIIKAVEEAFGVTATPIVAAGAAGATQEAASEVSVKVTGYADNAKLAVLKLYREITGVGLMEAKTAVEKLPCVVKQDIKPEEAEELKKRFVEVGATVEVK", "aa_hexdigest": "2a89c681ac8f1bc6764b695478e7c7bb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001341B3", "ncbi_nrp_id": "WP_009885819.1", "uniref100_id": "UniRef100_P36255", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001341B3", "RefSeq:WP_009885819.1", "UniRef:UniRef100_P36255" ] }, "ips": { "uniref100_id": "UniRef100_P36255", "uniref90_id": "UniRef90_P36255" }, "psc": { "uniref90_id": "UniRef90_P36255", "gene": "rplL", "product": "50S ribosomal protein L7/L12", "uniref50_id": "UniRef50_P36255", "cog_id": "COG0222", "cog_category": "J", "go_ids": [ "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P36255", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P36255" ], "product": "Large ribosomal subunit protein bL12" }, "genes": [ "rplL" ], "id": "JPCNHPFPBM_836", "locus": "GCA000292505_00835" }, { "type": "cds", "sequence": "contig_1", "start": 459989, "stop": 460162, "strand": "+", "frame": 2, "gene": "rpmF", "product": "50S ribosomal protein L32", "db_xrefs": [ "COG:COG0333", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "RefSeq:WP_009885820.1", "SO:0001217", "UniParc:UPI0000165F83", "UniRef:UniRef100_P47603", "UniRef:UniRef50_P47603", "UniRef:UniRef90_P47603" ], "nt": "ATGGCAGTACAACAACGGCGTTCTAGTAAACACCGTCGTGATAAAAGACGTTCTCACGATGCACTTACTCTACAAACTTTAAGTGTTTGTAAGAAATGTGGTAAGAAGAAGTTATCACATCGTGTGTGCTCTTGTGGTATGTACGGTGAACTAAGAGTTAAAAAAGCTCACTAA", "aa": "MAVQQRRSSKHRRDKRRSHDALTLQTLSVCKKCGKKKLSHRVCSCGMYGELRVKKAH", "aa_hexdigest": "cf34ba30d94ac5e4214a0fce93736145", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000165F83", "ncbi_nrp_id": "WP_009885820.1", "uniref100_id": "UniRef100_P47603", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000165F83", "RefSeq:WP_009885820.1", "UniRef:UniRef100_P47603" ] }, "ips": { "uniref100_id": "UniRef100_P47603", "uniref90_id": "UniRef90_P47603" }, "psc": { "uniref90_id": "UniRef90_P47603", "gene": "rpmF", "product": "50S ribosomal protein L32", "uniref50_id": "UniRef50_P47603", "cog_id": "COG0333", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_P47603", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47603" ], "product": "Large ribosomal subunit protein bL32" }, "genes": [ "rpmF" ], "id": "JPCNHPFPBM_837", "locus": "GCA000292505_00836" }, { "type": "cds", "sequence": "contig_1", "start": 460177, "stop": 460443, "strand": "-", "frame": 1, "gene": "rpsT", "product": "30S ribosomal protein S20", "db_xrefs": [ "COG:COG0268", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904", "KEGG:K02968", "RefSeq:WP_009885821.1", "SO:0001217", "UniParc:UPI0000134E49", "UniRef:UniRef100_P55750", "UniRef:UniRef50_P55750", "UniRef:UniRef90_P55750" ], "nt": "ATGGCTAATATTAAATCTAACGAAAAACGATTACGTCAAGACATTAAGAGAAATTTAAATAATAAAGGACAAAAAACTAAACTAAAAACTAATGTTAAAAAATTTAATAAAGAGATTAATTTAGATAATCTCAGTTCTGTTTATTCTCAAGCAGATCGTTTAGCCAGAAAAGGGATTATTTCTTTAAACAGAGCTAAGCGTTTAAAATCAAAAAATGCTGTTATTTTGCATAAAAGTAATACAAATTCAACTGCAAAAAAACAATAA", "aa": "MANIKSNEKRLRQDIKRNLNNKGQKTKLKTNVKKFNKEINLDNLSSVYSQADRLARKGIISLNRAKRLKSKNAVILHKSNTNSTAKKQ", "aa_hexdigest": "5720b3c8f1e572f2bd77a0be8e9a894d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134E49", "ncbi_nrp_id": "WP_009885821.1", "uniref100_id": "UniRef100_P55750", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134E49", "RefSeq:WP_009885821.1", "UniRef:UniRef100_P55750" ] }, "ips": { "uniref100_id": "UniRef100_P55750", "uniref90_id": "UniRef90_P55750" }, "psc": { "uniref90_id": "UniRef90_P55750", "gene": "rpsT", "product": "30S ribosomal protein S20", "uniref50_id": "UniRef50_P55750", "cog_id": "COG0268", "cog_category": "J", "kegg_orthology_id": "K02968", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P55750", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P55750" ], "product": "Small ribosomal subunit protein bS20" }, "genes": [ "rpsT" ], "id": "JPCNHPFPBM_838", "locus": "GCA000292505_00837" }, { "type": "cds", "sequence": "contig_1", "start": 460507, "stop": 460662, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGGTGGACAACAAGGGAGTTTTTTTGGGCTTTTAGTAATTGTTATTCCAATAATTTTGTTAATTGTTTTTTTTTCCAAGAAAAAAGGGGCACAAAAAAATGATTTTAGTGGTGAAGGAGGTAATCGATCATCAAGAAAAGATGAAGTGTGA", "aa": "MDGGQQGSFFGLLVIVIPIILLIVFFSKKKGAQKNDFSGEGGNRSSRKDEV", "aa_hexdigest": "c9c382566edc608eb2106c3ee9515271", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5529.328600000002, "isoelectric_point": 9.397443580627442 }, "id": "JPCNHPFPBM_839", "locus": "GCA000292505_00838" }, { "type": "cds", "sequence": "contig_1", "start": 460681, "stop": 461037, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MG364", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47604", "UniRef:UniRef90_P47604" ], "nt": "TTGCAAGAAAAGAATGAACGTGGTAAAGAAATTATTAAAACTTTTGTAGCTAAAAAACCAAACCCTTTGCATTCAAAAAAAGACCGCAAGCTTTTCAATCAAGAGATACAGGCATATATTACTAGTAATAACTTAGGAAAAAGCGAAGCAAAACGTTATAAAAATGAGCAAACTCGCTTAATGCAAAGAGAACTTTATTGTATTTATTTTGTTACAAAAGATGCTAAATCAACTGAAGTTGATGATGCTAGGATTATAGAAGCTGAGGTTTATCAAAAACCTACAAAAACCAAAAGTACTCCAGAGCGGCTAATTCGTATACTTGGTTTAAAAAATTTTGAAACTGAAATGCAATGA", "aa": "MQEKNERGKEIIKTFVAKKPNPLHSKKDRKLFNQEIQAYITSNNLGKSEAKRYKNEQTRLMQRELYCIYFVTKDAKSTEVDDARIIEAEVYQKPTKTKSTPERLIRILGLKNFETEMQ", "aa_hexdigest": "1f6d90f48589cd81fa683cff549a11aa", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 460507, "stop": 461181, "observations": { "mutation": [ 460660, 461035 ], "stop": [ 460660, 461035 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47604", "query_cov": 1.0, "subject_cov": 0.5267857142857143, "identity": 0.992, "score": 228.0, "evalue": 5.04e-74, "gene_start": 1, "gene_end": 118, "reference_start": 59, "reference_end": 176, "reference_sequence": "MDGGQQGSFFGLLVIVIPIILLIVFFSKKKGAQKNDFSGEGGNRSSRKDEVWKTIKQFLQEKNERGKEIIKTFVAKKPNPLHSKKDRKLFNQEIQAYITSNNLGKSEAKRYKNEQTRLMQRELYCIYFVTKDAKSTEVDDARIIEAEVYQKPTKTKSTPERLIRILGLKNFETEMQWIQPLMVREEKRKEKEEQKKLKLAARELKKKKKKKIKKPKEIRNQKNV" }, "paralog": false, "identity": 0.9866071428571429, "score": 341.0, "evalue": 2.85e-121, "description": "Internal stop codon at 460660, 461035. Nonsense mutation around 460660, 461035.", "psc": { "uniref90_id": "UniRef90_P47604", "product": "Uncharacterized protein MG364", "uniref50_id": "UniRef50_P47604", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47604", "UniRef:UniRef50_P47604" ] }, "pscc": { "uniref50_id": "UniRef50_P47604", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47604" ], "product": "Uncharacterized protein MG364" } }, "id": "JPCNHPFPBM_840", "locus": "GCA000292505_00839" }, { "type": "cds", "sequence": "contig_1", "start": 461050, "stop": 461181, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTCGTGAAGAAAAGAGAAAAGAAAAAGAAGAACAGAAAAAACTTAAATTAGCTGCAAGAGAACTAAAAAAGAAGAAAAAGAAGAAAATAAAAAAACCAAAAGAAATCAGAAATCAGAAAAATGTTTAA", "aa": "MVREEKRKEKEEQKKLKLAARELKKKKKKKIKKPKEIRNQKNV", "aa_hexdigest": "fb7dc0fd268b06683a3ce1714668d93a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5344.465800000003, "isoelectric_point": 10.49488887786865 }, "id": "JPCNHPFPBM_841", "locus": "GCA000292505_00840" }, { "type": "cds", "sequence": "contig_1", "start": 461174, "stop": 461539, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00551.24" ], "nt": "ATGTTTAAAATTGTTTTCTTTGGTACTTCAACGCTTTCAAAAAAATGTTTAGAACAACTTTTTTACGATAATGATTTTGAAATTTGTGCTGTTGTAACTCAGCCAGACAAAATTAATCATCGTAACAATAAAATAGTACCTTCTGATGTTAAGTCTTTTTGTTTGGAAAAAAACATAACTTTTTTTCAACCAAAACAAAGCATAAGCATAAAAGCTGATCTAGAAAAATTAAAAGCTGATATTGGTATTTGCGTTTCATTTGGCCAGTATCTTCATCAAGATATTATTGATCTTTTTCCAAATAAAGTAATTAACTTACATCCTTCTAAGTTACCACTACTTCGTGGTGGTGCACCATTACATTGA", "aa": "MFKIVFFGTSTLSKKCLEQLFYDNDFEICAVVTQPDKINHRNNKIVPSDVKSFCLEKNITFFQPKQSISIKADLEKLKADIGICVSFGQYLHQDIIDLFPNKVINLHPSKLPLLRGGAPLH", "aa_hexdigest": "905fc30a3e5ee055ff22e215af100c8d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00551.24", "name": "Formyl_trans_N", "length": 123, "aa_cov": 0.9752066115702479, "hmm_cov": 0.975609756097561, "evalue": 1.3215691172726287e-17, "score": 59.202640533447266, "start": 3, "stop": 120 } ], "seq_stats": { "molecular_weight": 13764.949899999981, "isoelectric_point": 8.693255424499512 }, "id": "JPCNHPFPBM_842", "locus": "GCA000292505_00841" }, { "type": "cds", "sequence": "contig_1", "start": 462111, "stop": 463052, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00031D7E73", "UniRef:UniRef90_UPI00031D7E73" ], "nt": "ATGATTACAAAGAGTTTTTTCCTTAAAAATTTTGATCGCTCTAAAGAATTAATTCCTCTTTCATATAACGATGTTTTTAGCGCTGTTAATCAGTTTCTGAAAAGCTATACTGACGTTAATGATATTGATATTATTGAAGAAAATTTGATTGAAGATCCAGTTTTTGAACTTGATGTTTTAGAACTTTTAAACGAGGATCCAATGCTATTATTGGACAGTAAAAATCCCCGTGTTCAAGAAGCTAAAATTATTGCTAATAAAGCAAAAAAAAATATTAAAGATTATTTTAATCTTCCCATTTTTTTTGATACAGATAGTCTAGATAAAAATGTTTCTGTTTATCAAAAAGCTGAGCTTACTGAAAAGAAAATTCAAAAAATAATAACATCAAAAAAATCAGCTATTATTTTTAAACCTATTTTTGAAATAGAAGATTGCTTAATTCAACCAGATGCTATTATTGTTCATGAAAAAGGACTTTGTGAATTTGTTGTTATAAAAGCTACGACCAATACAAAAAGAAAATATTTTTTGGAAATAATTTATGACTTTGTGTTATTTAAAAAAATAGGTAAGTATAAACTTTTAAACTATTATTTTTGTACTGTTAAATACGAATTACAAAATAAAAATAATGTTTCTTTCTTTTTAAATACTGAAATAAAAACATCAAAAAACAGTTTTAGTTTAAGTTCAAAAGAAAAAGATTATTTTAAGAATAAACCTTTTAATCACCCTGAAAAAATTGCTTACATACACAAAAAAAAGAGCAATGGAGTTAATGGTTTTTTGATTGTAAAACTCATTGATAACCTTATTAAAAACAATATAGTTGATTTAAACAAAATAAGTGATTTTGTTACTAAAGAAATTGACAGTAAAAGTGTTCGTAATATTCAACCACTTATTAAAAATGCGGCTAAAATACAAATTAATTTTTGA", "aa": "MITKSFFLKNFDRSKELIPLSYNDVFSAVNQFLKSYTDVNDIDIIEENLIEDPVFELDVLELLNEDPMLLLDSKNPRVQEAKIIANKAKKNIKDYFNLPIFFDTDSLDKNVSVYQKAELTEKKIQKIITSKKSAIIFKPIFEIEDCLIQPDAIIVHEKGLCEFVVIKATTNTKRKYFLEIIYDFVLFKKIGKYKLLNYYFCTVKYELQNKNNVSFFLNTEIKTSKNSFSLSSKEKDYFKNKPFNHPEKIAYIHKKKSNGVNGFLIVKLIDNLIKNNIVDLNKISDFVTKEIDSKSVRNIQPLIKNAAKIQINF", "aa_hexdigest": "c08f27544519afa5ee5c12b7af59abeb", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 462111, "stop": 463355, "observations": { "mutation": [ 463050, 463227 ], "stop": [ 463050, 463227 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00031D7E73", "query_cov": 1.0, "subject_cov": 0.6879120879120879, "identity": 1.0, "score": 598.0, "evalue": 1.7e-213, "gene_start": 1, "gene_end": 313, "reference_start": 1, "reference_end": 313, "reference_sequence": "MITKSFFLKNFDRSKELIPLSYNDVFSAVNQFLKSYTDVNDIDIIEENLIEDPVFELDVLELLNEDPMLLLDSKNPRVQEAKIIANKAKKNIKDYFNLPIFFDTDSLDKNVSVYQKAELTEKKIQKIITSKKSAIIFKPIFEIEDCLIQPDAIIVHEKGLCEFVVIKATTNTKRKYFLEIIYDFVLFKKIGKYKLLNYYFCTVKYELQNKNNVSFFLNTEIKTSKNSFSLSSKEKDYFKNKPFNHPEKIAYIHKKKSNGVNGFLIVKLIDNLIKNNIVDLNKISDFVTKEIDSKSVRNIQPLIKNAAKIQINFWDQIQDIKKYQELKINQIVFNYSENFDSFWSNYLLRNLIKLVFAHKYNEIFKLSGKLANWSQLTYAYKENKSITINQLLHELNQKKSKANFNNSTNKISFFLEAWNSEKGFAIGNKFKNTWNKLKKRKFILILKQLVHQSES" }, "paralog": false, "identity": 0.9951690821256038, "score": 811.0, "evalue": 7.57e-300, "description": "Internal stop codon at 463050, 463227. Nonsense mutation around 463050, 463227.", "psc": { "uniref90_id": "UniRef90_UPI00031D7E73", "uniref50_id": "UniRef50_UPI00031D7E73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00031D7E73", "UniRef:UniRef50_UPI00031D7E73" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00031D7E73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00031D7E73" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 36505.021700000005, "isoelectric_point": 9.025461006164551 }, "id": "JPCNHPFPBM_843", "locus": "GCA000292505_00842" }, { "type": "cds", "sequence": "contig_1", "start": 463706, "stop": 463987, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTACATTGATTAATGAACAAATATATAAAGAAAAAGTAAAAGCTATTGTTGACAATCTTTTTGACTTAGCTGATATTTTTACTATTGAAAATAACTGTTTGGCTTTCAAACAGCTAAACGGTTTTTCCTCTATCAAAAAGGTTTTAACTATTATAGATGAAAGCTTTTTAAAGGCAAGTAAATCAATTGGTTATCAAAATTTAAAAATCCAAAAAGGTGATGTAGCACAAGAAGTTGCTTTATCGCGTTTTCTAAATTGCTTAAATAAAAATGAATGA", "aa": "MATLINEQIYKEKVKAIVDNLFDLADIFTIENNCLAFKQLNGFSSIKKVLTIIDESFLKASKSIGYQNLKIQKGDVAQEVALSRFLNCLNKNE", "aa_hexdigest": "02ef597de60abf94d47f042022153718", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10515.09699999999, "isoelectric_point": 7.716768455505371 }, "id": "JPCNHPFPBM_844", "locus": "GCA000292505_00843" }, { "type": "cds", "sequence": "contig_1", "start": 464137, "stop": 464259, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTCTTTTACAAGTTTTTTAAAAAGTAGTAAAGGAAGCCATTTTTTTAGTGATTTAAAAGAGAAGATAGAAAATCAAAAGATGTGTAAGAAACTAGCTATTAAACCTAAAAAAAATTAG", "aa": "MASFTSFLKSSKGSHFFSDLKEKIENQKMCKKLAIKPKKN", "aa_hexdigest": "57e6d5bc23b1c41667eb7dd61ca46cd1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4606.4355000000005, "isoelectric_point": 10.015501976013184 }, "id": "JPCNHPFPBM_845", "locus": "GCA000292505_00844" }, { "type": "cds", "sequence": "contig_1", "start": 464320, "stop": 464616, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGCTAAAATTAGGTGATTTTGTTAAGTTAAGCAATGGTGCTGAACTAACTGAAAACACTGTTGGTGATGTACTTGAAGCTTTGGTTGGCGCCATTTATGAAGATATGGGGATGAAAAAAGCAACTGAATTTGTTGAAAAATATATCTTTGAAAGAACTTTTTCTGAGATTTTAAAATACGATTTCTTTTCACTTTTTCAAGAGCAAAAATTACCTGAACCAAGAGTTAGGGTAAGCTTAACTTCAAATAATTTGGTACTTAGTATAATTGAACTTGATGGTGATATTATTTGA", "aa": "MELKLGDFVKLSNGAELTENTVGDVLEALVGAIYEDMGMKKATEFVEKYIFERTFSEILKYDFFSLFQEQKLPEPRVRVSLTSNNLVLSIIELDGDII", "aa_hexdigest": "df634c168a522092a11f0653ccece72c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11154.69699999999, "isoelectric_point": 4.43170223236084 }, "id": "JPCNHPFPBM_846", "locus": "GCA000292505_00845" }, { "type": "cds", "sequence": "contig_1", "start": 464915, "stop": 465901, "strand": "-", "frame": 3, "gene": "plsX", "product": "phosphate acyltransferase PlsX", "db_xrefs": [ "COG:COG0416", "COG:I", "EC:2.3.1.274", "GO:0005737", "GO:0006633", "GO:0008610", "GO:0008654", "GO:0043811", "KEGG:K03621", "RefSeq:WP_009885824.1", "SO:0001217", "UniParc:UPI0000131C65", "UniRef:UniRef100_Q49427", "UniRef:UniRef50_Q49427", "UniRef:UniRef90_Q49427" ], "nt": "ATGGCTTTTAGATTTGCAGTTGATTGTTTAGGTTTTGAAAACAAACCTAGTGAAGCAATTGAAGCAGTATTGAAATATTGGAGTTTTCATCAGGACTTAAATTTCATTCTGATCGGTGATGAAAAGGCTTTTGATGGTCTTGATATACTTCCAAAAAATATTACAAAAAAACTTGCTAATTCTTTCATTGAAATGACCGACACTCCACTAAGTGCAAGAAGAAAAGTTAACAGTTCAATGCAAATAGCCATAAACTTAGTTCGTGAAGGTAATGCTGATGTTGTAATTTCAGCAGGCTCTTCAGCAGTTTATGCTTCTTTAACAAATGATGCTTTTGGCAAAATTAATAAAAATACTAAGAGTGCTTTTATGTCTTATGTACCGACAGATAACAATAATTGGTTTTATTTTTTGGATGTTGGAGCTAATAAATATTTCACAGGAAAAGAACTTTATTTTTTAGGTTTAATGGCTGATATTTTTGTTAAAAAAACAACTACAAAAAAAACTCCTAAAATTGGACTATTGAATATAGGAACAGAAGAAAATAAAGGTTTTGATTATCATCAAGAAGCATTTAAGCTTTTAAAAGCTGATAAAAATCTTAACTTTTTAGGATTTGTAGAATCAAGGTTTTTACTGGATGGAATATGTGATATTTTGATAGCTGATGGATATAGCGGCAATTTAGTTTTAAAAGCAATGGAAGGTACTTTTAAAACGATAGCACGGATTCTTAAGAGGGGTTATAAAAGAAATCCTCTTGCTGGCTTATTTAGCTTAGGTATTATCAAAAGTGTAGCCAAAAAATTTGATTACAAAAATAACGCTGGAGCTGTTGTAATGGGTTTAAATAAATTAGCTTTAAAAACTCACGGTTCTGCTGATAAACAACAATTTTTAAGCACGATTAGATTAGCACATCTAAGCTTAAAATCTGATCTTATTAATGCAATTAAAACTTCTTTGAATAACTATGAAGAATAA", "aa": "MAFRFAVDCLGFENKPSEAIEAVLKYWSFHQDLNFILIGDEKAFDGLDILPKNITKKLANSFIEMTDTPLSARRKVNSSMQIAINLVREGNADVVISAGSSAVYASLTNDAFGKINKNTKSAFMSYVPTDNNNWFYFLDVGANKYFTGKELYFLGLMADIFVKKTTTKKTPKIGLLNIGTEENKGFDYHQEAFKLLKADKNLNFLGFVESRFLLDGICDILIADGYSGNLVLKAMEGTFKTIARILKRGYKRNPLAGLFSLGIIKSVAKKFDYKNNAGAVVMGLNKLALKTHGSADKQQFLSTIRLAHLSLKSDLINAIKTSLNNYEE", "aa_hexdigest": "7153b7d2f88026ae33926ff1dfa6f4e8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000131C65", "ncbi_nrp_id": "WP_009885824.1", "uniref100_id": "UniRef100_Q49427", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000131C65", "RefSeq:WP_009885824.1", "UniRef:UniRef100_Q49427" ] }, "ips": { "uniref100_id": "UniRef100_Q49427", "uniref90_id": "UniRef90_Q49427" }, "psc": { "uniref90_id": "UniRef90_Q49427", "gene": "plsX", "product": "phosphate acyltransferase PlsX", "ec_ids": [ "2.3.1.274" ], "uniref50_id": "UniRef50_Q49427", "cog_id": "COG0416", "cog_category": "I", "kegg_orthology_id": "K03621", "go_ids": [ "GO:0005737", "GO:0006633", "GO:0008610", "GO:0008654", "GO:0043811" ] }, "pscc": { "uniref50_id": "UniRef50_Q49427", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49427" ], "product": "Phosphate acyltransferase" }, "genes": [ "plsX" ], "id": "JPCNHPFPBM_847", "locus": "GCA000292505_00846" }, { "type": "cds", "sequence": "contig_1", "start": 465901, "stop": 467055, "strand": "-", "frame": 1, "gene": "fakA", "product": "putative protein MG369", "db_xrefs": [ "COG:COG1461", "COG:I", "GO:0004371", "GO:0006071", "SO:0001217", "UniRef:UniRef50_P75231", "UniRef:UniRef90_P47609" ], "nt": "GTGAAAAAAACTCCCCAAATGTTACCTGTATTAAAAGCATCAGGTGTGGTTGATTCAGGTGCTTATGGTTTTGCTTGTTTTCTTGAAGGAATGTTGTCATATTATGGGGGCGAATCTAATTTAGATGACAATACTTTATCAACTATAGAAATTAAATTTAACAAGTTTAAAGAACAACATGCTAATGAAGATGAATTTGGTTATTGTACAGAATATGTTTTAAGACTAGGTTTAAAAATAAATCAGACAGTTGAAAAGCAAAAATTTCATCAAAAAAAGTTTGAAAGTAAAGTAAATAGAATTGCCAATTCTGTAGTAATTGCTAGTGATAAAGACAATGGTTTTGTTAAAGTTCATGCTCATACTTTAAAACCACATCTTTTATTAGAAATGGGTCTTAATTATGGTGAATTTGAATTTGTCAAAATTGATAACATGAATTTACAGGTTAATAACAAGAATAACTCACCAACAAAAAGAGTTTTAAAACCTGCTATAGTAGCTACTGTACCTACAGAAGCATTTGCTGAAAGAATTCGTGAAGATCATGATATTAACGCCATATTATGTACTGATGATACTGGGGCTCCTTCTGTTTTTTCTTTACTTGAGGCTGTTAAGTTAACCAATTCTAGTAATGTAATCTTTTTGTTACATGATAAAAACTATTTTCTATCTGCCAATGAAACAATTAAACAGTTAAAACACCAAAAGATTAATGCAGATTATGTTATTACAGCAAATCCTGTGGAGTCAATTGCTGCTTTAACTGTATTTAACAGTGATCTTTCTATTCACACTAATGTGAAAGCAATGAAGCGCTTTATTAAAGAGTTTGCTTCCGCTACGATAACACAAGCTTCAAAGTCATATAAAGAAAACAAAGTAATGGTGAATAAAAATGATTTTATTGCTGTTACAAATAAAAGTATTATTGCTAGTGAATCTCAACTTACAGACTGTTTTTTTAAAACAATTGATATCCTTTCAAAAAAAGTGAAAAAACCTGAGTTCCTTTTGGCTTATTATGGCAAAGACATCACTGAACAAAATGCGAAAAAAATGCAAGCTTTAGTTGAAAAAAAATATAAATTGTTTTGTGAATTTTCACCTGGAGAACAAAAAGTGTTCTCTTACATAATAGGCATACAGTAA", "aa": "MKKTPQMLPVLKASGVVDSGAYGFACFLEGMLSYYGGESNLDDNTLSTIEIKFNKFKEQHANEDEFGYCTEYVLRLGLKINQTVEKQKFHQKKFESKVNRIANSVVIASDKDNGFVKVHAHTLKPHLLLEMGLNYGEFEFVKIDNMNLQVNNKNNSPTKRVLKPAIVATVPTEAFAERIREDHDINAILCTDDTGAPSVFSLLEAVKLTNSSNVIFLLHDKNYFLSANETIKQLKHQKINADYVITANPVESIAALTVFNSDLSIHTNVKAMKRFIKEFASATITQASKSYKENKVMVNKNDFIAVTNKSIIASESQLTDCFFKTIDILSKKVKKPEFLLAYYGKDITEQNAKKMQALVEKKYKLFCEFSPGEQKVFSYIIGIQ", "aa_hexdigest": "a804c49b1d63b58a75cd49d16fc28cbe", "start_type": "GTG", "rbs_motif": null, "genes": [ "fakA" ], "truncated": "3-prime", "pseudogene": { "start": 465901, "stop": 467355, "observations": { "mutation": [ 467064 ], "stop": [ 467064 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47609", "query_cov": 1.0, "subject_cov": 0.6894075403949731, "identity": 0.995, "score": 749.0, "evalue": 1.82e-270, "gene_start": 1, "gene_end": 384, "reference_start": 174, "reference_end": 557, "reference_sequence": "MSSVNLSSFIDMLRLGCNNIAKNYEYINQLNVFPVPDGDTGTNMKVTITEAIKKLENEKSHIKSFSELGKNFTRDLLLFSRGNSGVIFSQIMKGFFSNIIVNKTSNNSELSIEDVANAFIVAQEVAYKNVSKPVEGTMLTVARLISNEFKSQKNRPKTLEKLFEQAVKVAWQAVKKTPQMLPVLKASGVVDSGAYGFACFLEGMLSYYGGESNLDDNTLSTIEIKFNKFKEQHANEDEFGYCTEYVLRLGLKINQTVEKQKFHQKKFESKVNRIANSVVIASDKDNGFVKVHAHTLKPHLLLEMGLNYGEFEFVKIDNMNLQVNNKNNSPTKRVLKPAIVATVPTEAFAERIREDHDINAILCTDDTGAPSVFSLLEAVKLTNSSNVIFLLHDKNYFLSANETIKQLKHQKINADYVITANPVESIAALTVFNSDLSIHTNVKAMKRFIKEFASATITQASKSYKENKVMVNKNDFIAVTNKSIIASESQLTDCFFKTIDILSKKVKKPEFLLAYYGKDITEQDAKKMQALVEKKYKLFCEFSPGEQKVFSYIIGIQ" }, "paralog": false, "identity": 0.9958677685950413, "score": 939.0, "evalue": 0.0, "description": "Internal stop codon at 467064. Nonsense mutation around 467064.", "psc": { "uniref90_id": "UniRef90_P47609", "gene": "FakA", "product": "Uncharacterized protein MG369", "uniref50_id": "UniRef50_P75231", "cog_id": "COG1461", "cog_category": "I", "go_ids": [ "GO:0004371", "GO:0006071" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47609", "UniRef:UniRef50_P75231", "COG:COG1461", "COG:I", "GO:0004371", "GO:0006071" ] }, "pscc": { "uniref50_id": "UniRef50_P75231", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75231" ], "product": "Uncharacterized protein MG369 homolog" } }, "id": "JPCNHPFPBM_848", "locus": "GCA000292505_00847" }, { "type": "cds", "sequence": "contig_1", "start": 467062, "stop": 467574, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCATCAGTAAATTTATCTTCTTTTATCGATATGTTACGCCTTGGCTGTAATAACATCGCTAAAAATTATGAATACATAAACCAGTTGAATGTTTTTCCTGTTCCTGATGGTGACACTGGAACAAACATGAAAGTAACAATAACAGAAGCCATCAAGAAACTAGAAAATGAAAAAAGTCATATAAAAAGCTTTAGTGAACTTGGTAAAAACTTTACTAGAGATTTACTTTTGTTTTCACGTGGCAATTCAGGGGTTATCTTTTCACAAATAATGAAAGGATTTTTCAGTAACATAATCGTAAATAAAACAAGCAATAATTCAGAGTTGAGTATAGAAGATGTTGCAAATGCATTCATTGTTGCTCAAGAAGTGGCTTATAAAAACGTATCAAAACCAGTAGAAGGAACTATGCTAACAGTTGCTCGTTTAATAAGCAATGAGTTTAAGAGTCAAAAAAATCGTCCTAAAACACTTGAAAAACTTTTTGAACAAGCTGTTAAAGTAGCTTGA", "aa": "MSSVNLSSFIDMLRLGCNNIAKNYEYINQLNVFPVPDGDTGTNMKVTITEAIKKLENEKSHIKSFSELGKNFTRDLLLFSRGNSGVIFSQIMKGFFSNIIVNKTSNNSELSIEDVANAFIVAQEVAYKNVSKPVEGTMLTVARLISNEFKSQKNRPKTLEKLFEQAVKVA", "aa_hexdigest": "3d8ff73212fe5eef2bbff17ed045ab71", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18991.6027, "isoelectric_point": 9.16187610626221 }, "id": "JPCNHPFPBM_849", "locus": "GCA000292505_00848" }, { "type": "cds", "sequence": "contig_1", "start": 467643, "stop": 467912, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTTTAATAACAATTAGATTAATTAGTGGTAGGACTCACCAAATTCGTGCGTGTTTAAATTTATTTTCAAATCAACTAGTTGGTGATAAAAAATACAGCTTGATTCAATTTAAAAATCGTAATAATAAATATAAGCATCAAGCACTTCACGCTTATGAATTAGTTTTTCCTAAACTAGAAACGAGCAAGTTTCCCATTTTAACAAATTATTCATTAATGCAATTCAAGAGTAAAATAGTACCTTGGTTTGAATATTTAATTCAATAG", "aa": "MSLITIRLISGRTHQIRACLNLFSNQLVGDKKYSLIQFKNRNNKYKHQALHAYELVFPKLETSKFPILTNYSLMQFKSKIVPWFEYLIQ", "aa_hexdigest": "e85fdb441f3f920480a09b477d5db21e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10607.384099999994, "isoelectric_point": 10.00628299713135 }, "id": "JPCNHPFPBM_850", "locus": "GCA000292505_00849" }, { "type": "cds", "sequence": "contig_1", "start": 468021, "stop": 468614, "strand": "-", "frame": 2, "gene": "rluA", "product": "putative RNA pseudouridine synthase MG370", "db_xrefs": [ "COG:COG0564", "COG:J", "EC:5.4.99.-", "GO:0000455", "GO:0003723", "GO:0009982", "GO:0140098", "SO:0001217", "UniRef:UniRef50_P47610", "UniRef:UniRef90_P47610" ], "nt": "ATGAGAATAGCTAATAAATTTTTAGTTCCTAAAGAAAGTGAAAACCAACGAATTGACCAATTTTGTTTAAAAATCCTTCCCTTAATTAAAAGAAGTGACTTTTTTAAGTATTTACGCTTAGGAAAGGTTTTGTTAAATAAAACAAAACCACAGGTCAACACAAGATTAAAAACTAAAGATGAGATAGCTTTTCTTTTTGATATAAATCCCTATTTACAAACTAATAATGACTATTTATCTCTAGATTTGGTAAAAGATAAGCTAAAAATTATATTTGAGGATGAAAATATTATTGTAGTTGACAAACCAACTGGAATAGTTTGCCAACCTGATAAAAAACATAGCATTATTAATTTATCAAATATGCTTTTAAAACACTGTGGATATAGACAATTTGACAGTAATAAACTTAACTTTTATCCCCAGTTTGCACATCGTATTGATCGTAATACTTCTGGAATTGTTATTGGTGCTAAAACAAACAAAGCTTTAAAAGAATTGAACAAAGTTTTTAAAAATAACCATTTAACCAAAAGATATAAAGGCTTAGTTTTTGGTCAATTTAACCATCTTGGCTTACAAACTGCTTATTGA", "aa": "MRIANKFLVPKESENQRIDQFCLKILPLIKRSDFFKYLRLGKVLLNKTKPQVNTRLKTKDEIAFLFDINPYLQTNNDYLSLDLVKDKLKIIFEDENIIVVDKPTGIVCQPDKKHSIINLSNMLLKHCGYRQFDSNKLNFYPQFAHRIDRNTSGIVIGAKTNKALKELNKVFKNNHLTKRYKGLVFGQFNHLGLQTAY", "aa_hexdigest": "cf3bab7d7220f2de23be0691d9409eea", "start_type": "ATG", "rbs_motif": null, "genes": [ "rluA" ], "truncated": "5-prime", "pseudogene": { "start": 467718, "stop": 468614, "observations": { "mutation": [ 467984, 468023 ], "stop": [ 467984, 468023 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47610", "query_cov": 1.0, "subject_cov": 0.6099071207430341, "identity": 1.0, "score": 396.0, "evalue": 8.17e-138, "gene_start": 1, "gene_end": 197, "reference_start": 1, "reference_end": 197, "reference_sequence": "MRIANKFLVPKESENQRIDQFCLKILPLIKRSDFFKYLRLGKVLLNKTKPQVNTRLKTKDEIAFLFDINPYLQTNNDYLSLDLVKDKLKIIFEDENIIVVDKPTGIVCQPDKKHSIINLSNMLLKHCGYRQFDSNKLNFYPQFAHRIDRNTSGIVIGAKTNKALKELNKVFKNNHLTKRYKGLVFGQFNHLGLQTAYWKKDNNNGIVTVKWKPFPEAKKISTFFENSSYIAQKDMSLITIRLISGRTHQIRACLNLFSNQLVGDKKYSLIQFKNRNNKYKHQALHAYELVFPKLETSKFPILTNYSLMQFKSKIVPWFEYLIQ" }, "paralog": false, "identity": 0.9932885906040269, "score": 593.0, "evalue": 9.05e-218, "description": "Internal stop codon at 467984, 468023. Nonsense mutation around 467984, 468023.", "psc": { "uniref90_id": "UniRef90_P47610", "gene": "RluA", "product": "Uncharacterized RNA pseudouridine synthase MG370", "ec_ids": [ "5.4.99.-" ], "uniref50_id": "UniRef50_P47610", "cog_id": "COG0564", "cog_category": "J", "go_ids": [ "GO:0140098", "GO:0009982", "GO:0003723", "GO:0000455" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47610", "EC:5.4.99.-", "UniRef:UniRef50_P47610", "COG:COG0564", "COG:J", "GO:0140098", "GO:0009982", "GO:0003723", "GO:0000455" ] }, "pscc": { "uniref50_id": "UniRef50_P47610", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47610" ], "product": "Uncharacterized RNA pseudouridine synthase MG370" } }, "id": "JPCNHPFPBM_851", "locus": "GCA000292505_00850" }, { "type": "cds", "sequence": "contig_1", "start": 468601, "stop": 468720, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCAATGGTGGTGGACATAAATATGCAGCTGCTTTTGTTTTGGATGAAAAAAATCAATTTATGAAATTAGTACAGATAATGGATGATTTTCTTGCCAAACAAAATGAGAATAGCTAA", "aa": "MFNGGGHKYAAAFVLDEKNQFMKLVQIMDDFLAKQNENS", "aa_hexdigest": "6349d3033f3deba959e08d9f64c10586", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4451.0236, "isoelectric_point": 5.498172187805174 }, "id": "JPCNHPFPBM_852", "locus": "GCA000292505_00851" }, { "type": "cds", "sequence": "contig_1", "start": 468769, "stop": 469023, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAACTGGTTTTAACCGCAATAAGGTTCATGATGCAGTTTATTTAAAGCCATTACTAGAACATAAGTACTTTAGCTATGTACTTAGCAAAGCCAAAATAACTAAAAATGGGTTAGCTTATGCATTGATTAAAAAAGGAGCATATAAACATTTTGGTGTTGTCTCTCCTTTACCAATGGTTCATGCTTTGAATAACATAAAAGGAGTAAAAATATGGACTACTGTTTACTTTAACGAGTCCATTAAAAAATGA", "aa": "METGFNRNKVHDAVYLKPLLEHKYFSYVLSKAKITKNGLAYALIKKGAYKHFGVVSPLPMVHALNNIKGVKIWTTVYFNESIKK", "aa_hexdigest": "3973dcb71ca265a51be8444ae6032056", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9612.270499999997, "isoelectric_point": 9.953547859191893 }, "id": "JPCNHPFPBM_853", "locus": "GCA000292505_00852" }, { "type": "cds", "sequence": "contig_1", "start": 469069, "stop": 469167, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGGTTATTTAATTTTGCAGATGGGTTACAAATTAAATGATGAGATTGCATCATATCTTTATGCAGGAATAATTACTGATACACAGCGATTCTGA", "aa": "MIGYLILQMGYKLNDEIASYLYAGIITDTQRF", "aa_hexdigest": "59cfb9f83911f8505c9aa190a1ea6976", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3715.294899999999, "isoelectric_point": 4.556577491760254 }, "id": "JPCNHPFPBM_854", "locus": "GCA000292505_00853" }, { "type": "cds", "sequence": "contig_1", "start": 469198, "stop": 469575, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01368.27" ], "nt": "ATGATTAGTATCGATCCACAATTCATTAAGAATTTTTCAAAAAAGGTTAAACAGTTTGATAAGTTCAGTTTATTTGTACATGTAAACCCAGACTTTGATGCATTTGGATCTGCTTTTGCTTTCAAAACTTTCCTAAATACTTTTTTCAGTGAAAAAAAAGCTTATGTTATGGGTTCATACAATATTAATGCAGACGGTAGAGAACTTTTTCCTTTTGAGCAAACTGACATAAATGATGATTTTGTAAAAGAATCTTTAGCTATTATTTTTGATACTTCCAACCAAGAAAGAGTTTTAACTCAAAAACATAAGTTGGCAAAAGAGACAGTTAGGATAGATCACCATCCTAGAACTGAAAAATTTGCTGATATGGAATGA", "aa": "MISIDPQFIKNFSKKVKQFDKFSLFVHVNPDFDAFGSAFAFKTFLNTFFSEKKAYVMGSYNINADGRELFPFEQTDINDDFVKESLAIIFDTSNQERVLTQKHKLAKETVRIDHHPRTEKFADME", "aa_hexdigest": "4cfe512bd2c9cd15a27e88cd27053a61", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01368.27", "name": "DHH", "length": 100, "aa_cov": 0.784, "hmm_cov": 0.75, "evalue": 4.5143251948672437e-11, "score": 38.24043655395508, "start": 24, "stop": 121 } ], "seq_stats": { "molecular_weight": 14628.388799999979, "isoelectric_point": 6.077304649353027 }, "id": "JPCNHPFPBM_855", "locus": "GCA000292505_00854" }, { "type": "cds", "sequence": "contig_1", "start": 469568, "stop": 470725, "strand": "-", "frame": 3, "gene": "thiI", "product": "putative tRNA sulfurtransferase", "db_xrefs": [ "COG:COG0301", "COG:HJ", "EC:2.8.1.4", "GO:0000049", "GO:0002937", "GO:0004810", "GO:0005524", "GO:0005829", "GO:0009228", "GO:0009229", "GO:0052837", "GO:0140741", "KEGG:K03151", "RefSeq:WP_009885937.1", "SO:0001217", "UniParc:UPI0000136E19", "UniRef:UniRef100_P47612", "UniRef:UniRef50_P47612", "UniRef:UniRef90_P47612" ], "nt": "ATGGAATTGAATAGTGAAGATGTATTAGTTGCGCGTTATGGTGAATTGGTTTTAAAAGGAAAAAACCGTTCTTATTTCACAAAACAATTAAAAATTAACATCAAAAAAGCTTTTAAAAAACTGGAAATTAACAATAGTATTGTTTATGAATTTGATAGAATTGTTGTCTTTGATATTAAAAAAGAACAAAGAGCGATTCTCCAAGAATTATTCAGTTTTTTGCCTGGAATTAGTTTGTTCTTTTTTGCATCACAGATAGTAAGGGAAGAAAACAAACTATTAGATCTTCTTTTTAATTTATTTAAAGATTTTAATTCCTTTAAATTAGAAGTTAAGAGACGTGATAAAAATTTTGCTGAAAATTCAAGTAATTTTAAGAAGTATTTAGCAGTTAAACTATTTGAAAAATACCAATTAAAAGGAGTAATTAATAACCCTGAAATAATAGCTAATATAGAGATTTTGAAGGAGCATTTTCTTGTTTTTACAGAACGTTTTAAAGGTAAAGGTGGATTACCTGTTTATAGTAGTGGTAAAGCTTTAGTCTTACTTTCAGGAGGAATTGATAGCCCAGTTGCAGCTTCACTTGTAATGCAACGTGGTTTTAACATTGATTTTATCACCTTTATTAATGAACCAAATAAGAATCAAAAAACAATTGAAAAAATCACAAGGTTAGCAAATTTAATTTCTTTTAATAAAACGATTTGTAGTGGTAAATTATTAGTTTTTGATTTCACTGCAATTCAAAAAGAGTTAATTCATATTAGTAATGAATCTTATCGTATCGTTTTAATGCGTCGTGTTTTTTATAAAGCTGCTAGTATGTTTAAGTATGATTGTCTAGTTACAGGTGAGGTTTTGGGACAAGTTGCATCCCAAACCATTGAAAATTTAAAAGTTATTCAATCAGCAACACCAGATACATTTATAGTTCGCCCCTTAATTGGCTTTAGTAAAGATAAAATTATTGAATTAGCTAAATTTTTTAATACTTTTGATATTTCTATTGAACAACATTTAGATACTTGTAGTGAGTTTTCTCCCAAAAATCCTACAACGAAAGCTAAATTGATAAATGTTGAAAAGCTTGAAAGTGAGCTTATTTTTTTAAATGATTTAATTGAAAAGGGAGTAAGTGAATTAAGCAATGATTAG", "aa": "MELNSEDVLVARYGELVLKGKNRSYFTKQLKINIKKAFKKLEINNSIVYEFDRIVVFDIKKEQRAILQELFSFLPGISLFFFASQIVREENKLLDLLFNLFKDFNSFKLEVKRRDKNFAENSSNFKKYLAVKLFEKYQLKGVINNPEIIANIEILKEHFLVFTERFKGKGGLPVYSSGKALVLLSGGIDSPVAASLVMQRGFNIDFITFINEPNKNQKTIEKITRLANLISFNKTICSGKLLVFDFTAIQKELIHISNESYRIVLMRRVFYKAASMFKYDCLVTGEVLGQVASQTIENLKVIQSATPDTFIVRPLIGFSKDKIIELAKFFNTFDISIEQHLDTCSEFSPKNPTTKAKLINVEKLESELIFLNDLIEKGVSELSND", "aa_hexdigest": "da46482a56b14043bb349542cd756c8a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000136E19", "ncbi_nrp_id": "WP_009885937.1", "uniref100_id": "UniRef100_P47612", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000136E19", "RefSeq:WP_009885937.1", "UniRef:UniRef100_P47612" ] }, "ips": { "uniref100_id": "UniRef100_P47612", "uniref90_id": "UniRef90_P47612" }, "psc": { "uniref90_id": "UniRef90_P47612", "gene": "thiI", "product": "Probable tRNA sulfurtransferase", "ec_ids": [ "2.8.1.4" ], "uniref50_id": "UniRef50_P47612", "cog_id": "COG0301", "cog_category": "HJ", "kegg_orthology_id": "K03151", "go_ids": [ "GO:0005829", "GO:0005524", "GO:0004810", "GO:0000049", "GO:0140741", "GO:0009228", "GO:0009229", "GO:0052837", "GO:0002937" ] }, "pscc": { "uniref50_id": "UniRef50_P47612", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47612" ], "product": "Probable tRNA sulfurtransferase" }, "genes": [ "thiI" ], "id": "JPCNHPFPBM_856", "locus": "GCA000292505_00855" }, { "type": "cds", "sequence": "contig_1", "start": 470796, "stop": 470957, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGCAATGTAAATAAAGGACTTTTTGCAATTGCCTTTTTGAAACCAGAAGATTATGTCTTACCAGAACAATTTAATGCTAGCAATAGAGAAATTAGGTTAGTTCCTATCAAGATAGATCATAAGGGATTTAGTGTTTTAGTTGATAAGGCGAGAATTTGA", "aa": "MRNVNKGLFAIAFLKPEDYVLPEQFNASNREIRLVPIKIDHKGFSVLVDKARI", "aa_hexdigest": "774c4502fc051e890c4788580664c866", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6112.1125, "isoelectric_point": 9.693288993835449 }, "id": "JPCNHPFPBM_857", "locus": "GCA000292505_00856" }, { "type": "cds", "sequence": "contig_1", "start": 471024, "stop": 471383, "strand": "-", "frame": 2, "gene": null, "product": "MPN551 family DNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75227", "UniRef:UniRef90_P47613" ], "nt": "ATGGTCAAAATTTATGAAGATGAATTTGATGAAACACTGGCAAAAGCTGGAAACATAATAGAACCTAAAATACGTGATTATGTCAATTTAAAAACTGGTTTTAATTTTCATTCTTATGATCCTAAAAAAGTTCAATTTGATTTATTTAAGGATGATAGTGTTTTTGGTGGGATACCTGATGGTGAACCTCTTGATGAAAATGGTGAATTAGCTTATCAAAATGATCTTCCTATGTTAGAGATTAAAACAACTTCTTGCGATAGTCTTATTTATAAAAAAATCAATGGAAATCTCAAGATGATTCTTGATGAAAATGGTATGCCTATTGTTAAAAAGAAAGATGGCAAAAAAGATAGTTGA", "aa": "MVKIYEDEFDETLAKAGNIIEPKIRDYVNLKTGFNFHSYDPKKVQFDLFKDDSVFGGIPDGEPLDENGELAYQNDLPMLEIKTTSCDSLIYKKINGNLKMILDENGMPIVKKKDGKKDS", "aa_hexdigest": "c7c7b9ad22adf906217200395552bd5b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 470721, "stop": 471557, "observations": { "mutation": [ 470738, 470798, 471026, 471392 ], "stop": [ 470738, 470798, 471026, 471392 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47613", "query_cov": 1.0, "subject_cov": 0.4234875444839858, "identity": 1.0, "score": 242.0, "evalue": 5.48e-79, "gene_start": 1, "gene_end": 119, "reference_start": 59, "reference_end": 177, "reference_sequence": "MGFIKQYKTDFDIVDNQIVLSEQYFLRNKALFKKITGTRFGKVLGLSEYESSFKTWANMVKIYEDEFDETLAKAGNIIEPKIRDYVNLKTGFNFHSYDPKKVQFDLFKDDSVFGGIPDGEPLDENGELAYQNDLPMLEIKTTSCDSLIYKKINGNLKMILDENGMPIVKKKDGKKDSWFDSNGKIIISTLYYCQIGLYLYLRNVNKGLFAIAFLKPEDYVLPEQFNASNREIRLIPIKIDHKGFSVLVDKARIWYENYILTGKSPKLTESDIQWLKENGIE" }, "paralog": false, "identity": 0.9820143884892086, "score": 545.0, "evalue": 9.75e-201, "description": "Internal stop codon at 470738, 470798, 471026, 471392. Nonsense mutation around 470738, 470798, 471026, 471392.", "psc": { "uniref90_id": "UniRef90_P47613", "product": "MPN551 family DNA-binding protein", "uniref50_id": "UniRef50_P75227", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47613", "UniRef:UniRef50_P75227" ] }, "pscc": { "uniref50_id": "UniRef50_P75227", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75227" ], "product": "Uncharacterized protein MG373 homolog" } }, "id": "JPCNHPFPBM_858", "locus": "GCA000292505_00857" }, { "type": "cds", "sequence": "contig_1", "start": 471390, "stop": 471557, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGATTTATAAAGCAATATAAAACTGATTTTGATATAGTTGATAATCAAATTGTTCTATCAGAACAGTATTTTTTAAGAAATAAAGCTCTATTTAAAAAAATAACCGGTACTCGCTTTGGCAAGGTTTTAGGTTTATCTGAATATGAGTCATCATTTAAAACTTGA", "aa": "MGFIKQYKTDFDIVDNQIVLSEQYFLRNKALFKKITGTRFGKVLGLSEYESSFKT", "aa_hexdigest": "7517bb11e32be49a605a3309da56ba93", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6444.3674999999985, "isoelectric_point": 9.397701454162597 }, "id": "JPCNHPFPBM_859", "locus": "GCA000292505_00858" }, { "type": "cds", "sequence": "contig_1", "start": 471558, "stop": 471914, "strand": "-", "frame": 2, "gene": null, "product": "MPN552 family protein", "db_xrefs": [ "GO:0005524", "SO:0001217", "UniRef:UniRef50_P75226", "UniRef:UniRef90_P47614" ], "nt": "ATGGATGTTAGTTCTATTTATGTATTTAGTCCTCAGTATCATTTAGACTTTCAACTTATGGAACATATTTCTAGTGGTCAGCCTAAGTTTCATTACAGCAATTTTTATAACGAAAATTTTTTATTTACTAGCACAAAAAATTCCAAGTTTAGTGCTTTAGAAACATATGTTAAAAGCAAGAATTGCTCTTTAAAAGATGTTCATTATTTAAATGTGACAGAAGTTCCAATTAATAACATTAATCAATTAGCAAGTGTTGTATTTTTAAGCAAAAAACAACCAGATAATGATGAGGAATTTAATAATCCTGAAATATCAATTGTTTTAAAAGGAATTTGTGAACAGTTGATGAAATAA", "aa": "MDVSSIYVFSPQYHLDFQLMEHISSGQPKFHYSNFYNENFLFTSTKNSKFSALETYVKSKNCSLKDVHYLNVTEVPINNINQLASVVFLSKKQPDNDEEFNNPEISIVLKGICEQLMK", "aa_hexdigest": "bc1bb6186708604d079a0f72d40bf52e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 471558, "stop": 472214, "observations": { "mutation": [ 471974, 472166 ], "stop": [ 471974, 472166 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47614", "query_cov": 1.0, "subject_cov": 0.4306569343065693, "identity": 1.0, "score": 236.0, "evalue": 7.99e-77, "gene_start": 1, "gene_end": 118, "reference_start": 157, "reference_end": 274, "reference_sequence": "MDRKIVVLDINTANFFNTTKDLEVWKNFFNFIQANNHQLVFMSSCWQQAVLYLLDLLSLDDVDIIAESGAIIWISKTNEFIYQSFLDSVSIDTIVHHAIITNSGVFAIGKSKISEEPNTTINYFISLEKYKQFKLIWLTEFDQTLKYQNFLKNLSEMDVSSIYVFSPQYHLDFQLMEHISSGQPKFHYSNFYNENFLFTSTKNSKFSALETYVKSKNCSLKDVHYLNVTEVPINNINQLASVVFLSKKQPDNDEEFNNPEISIVLKGICEQLMK" }, "paralog": false, "identity": 0.9908256880733946, "score": 426.0, "evalue": 5.85e-154, "description": "Internal stop codon at 471974, 472166. Nonsense mutation around 471974, 472166.", "psc": { "uniref90_id": "UniRef90_P47614", "product": "MPN552 family protein", "uniref50_id": "UniRef50_P75226", "go_ids": [ "GO:0005524" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47614", "UniRef:UniRef50_P75226", "GO:0005524" ] }, "pscc": { "uniref50_id": "UniRef50_P75226", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75226" ], "product": "Uncharacterized protein MG374 homolog" } }, "id": "JPCNHPFPBM_860", "locus": "GCA000292505_00859" }, { "type": "cds", "sequence": "contig_1", "start": 472308, "stop": 472436, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAAAATCACCAGAATTAGCTTTAATAATTTTGTTAAAAGAGTTAGAAGAGATGGATAGAAAAATAGTTGTTTTAGATATTAATACAGCAAATTTTTTTAACACTACAAAGGATTTAGAAGTATGA", "aa": "MKKSPELALIILLKELEEMDRKIVVLDINTANFFNTTKDLEV", "aa_hexdigest": "f87a55e59762405bae8c3c7f2ba4f7fe", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4876.728700000001, "isoelectric_point": 4.8126939773559565 }, "id": "JPCNHPFPBM_861", "locus": "GCA000292505_00860" }, { "type": "cds", "sequence": "contig_1", "start": 472375, "stop": 472959, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25", "PFAM:PF00587.30" ], "nt": "ATGGAGAATGTTTTAAAAGATTTAAATATCCAATATCAAAAAGAGATAGGAGCTGCTGCTTTTTATGGACCAAAAATTGATTTTCAGTTCAAAACAATCTTTAAAAAAATGATTACTATTGCCACCATTCAACTAGATTTTTTACTACCAGAAAAATTTGATCTAACTTATATAGATAAAAAAAATACACTAAAAAAACCAGTTATTATCCATGTTGGAATTATTGGAACTTATGAAAGGTTTATTGCTGCTTTACTTGAAAAAACAAGTGGTAATTTTCCTTTATGGTTAGCACCTGTTCAAGCCGTAATTATTCCTGTTAATATCCAAAAGCATTTAAAGGCAGCAAAAAAACTTTATAACAAATTGCTAAAAGAAAACATCCGTGTAAATTTAGATGATAATCAAGATCGCTTAGCTAAAAAAGTTAGACAAGCAATCATTGAAAAAATTCCTTTACAACTTATTGTTGGAGATAAAGAAATAGAGAATTTAGAGAAGTTGACATGCCGTGGTTTTAAAGGTGAAAAAATCACCAGAATTAGCTTTAATAATTTTGTTAAAAGAGTTAGAAGAGATGGATAG", "aa": "MENVLKDLNIQYQKEIGAAAFYGPKIDFQFKTIFKKMITIATIQLDFLLPEKFDLTYIDKKNTLKKPVIIHVGIIGTYERFIAALLEKTSGNFPLWLAPVQAVIIPVNIQKHLKAAKKLYNKLLKENIRVNLDDNQDRLAKKVRQAIIEKIPLQLIVGDKEIENLEKLTCRGFKGEKITRISFNNFVKRVRRDG", "aa_hexdigest": "2267b94af11ba60b986562197adba930", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 93, "aa_cov": 0.4690721649484536, "hmm_cov": 0.989247311827957, "evalue": 6.895786776410438e-22, "score": 72.59778594970703, "start": 101, "stop": 191 }, { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 92, "aa_cov": 0.44329896907216493, "hmm_cov": 0.9782608695652174, "evalue": 9.200194935497309e-16, "score": 53.49976348876953, "start": 3, "stop": 88 } ], "seq_stats": { "molecular_weight": 22423.315200000012, "isoelectric_point": 9.805141639709472 }, "id": "JPCNHPFPBM_862", "locus": "GCA000292505_00861" }, { "type": "cds", "sequence": "contig_1", "start": 472978, "stop": 473454, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "GTGCATCACCAGCAACTATTGTTTGGTTTTAATACTGTTTGTTCTCCTGTATTAGCTAACATAGAGCTTTTTAAAATTAGCGGCCACTATCAGCACTATAAGGAAGATATGTTTCCTGCTATTAAACTTGATAGTCAAGCAATGATGCTTCGTCCTATGACATGTCCTCATCACTGTTTGATTTTCAAACAAAAACGATATTCATATAAAAAAATGCCACAGCGCTTTTCAGAAGATTCTATTTTGCATCGTTTTGAAGCCTCTGGAGGATTAATAGGATTAGAAAGAGTGAGGTGCATGACTTTACTTGATAATCACATTTTTTGTCGTGCAGATCAAATTAAAAGTGAGATTAAAAACGCATTTAATTTAATTCAAAAAGTTAATAAAAAATTTGGATTTATATTTGATAGGATAGATCTTTCTCTACATGATCCTAAAAATCAATCAAAATTTATTGATAATCCTGGTTTATGA", "aa": "MHHQQLLFGFNTVCSPVLANIELFKISGHYQHYKEDMFPAIKLDSQAMMLRPMTCPHHCLIFKQKRYSYKKMPQRFSEDSILHRFEASGGLIGLERVRCMTLLDNHIFCRADQIKSEIKNAFNLIQKVNKKFGFIFDRIDLSLHDPKNQSKFIDNPGL", "aa_hexdigest": "01c2181845851cad57c667f04accbeb6", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 101, "aa_cov": 0.6329113924050633, "hmm_cov": 0.9801980198019802, "evalue": 4.9794789539025446e-18, "score": 60.883846282958984, "start": 46, "stop": 145 } ], "seq_stats": { "molecular_weight": 18530.4717, "isoelectric_point": 9.259739112854003 }, "id": "JPCNHPFPBM_863", "locus": "GCA000292505_00862" }, { "type": "cds", "sequence": "contig_1", "start": 473497, "stop": 473679, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGAGATCCTTCACAATTACAACTTCAGAGGATTAATGGCATTTTTGCTCAATCAAAAAAAGAATTAGAACAATTAATAAAAGAAAATGAAGAACGCTTGAAGAAGGATCACAGATCTTTAGGTAAACAATTAGAGTTATTTAGCTTTGACCCACTGATCGGTGCAGGTCTTCCTATTTGA", "aa": "MGDPSQLQLQRINGIFAQSKKELEQLIKENEERLKKDHRSLGKQLELFSFDPLIGAGLPI", "aa_hexdigest": "2d1ceafb525218cb246457be55844758", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6846.820599999997, "isoelectric_point": 6.556002616882325 }, "id": "JPCNHPFPBM_864", "locus": "GCA000292505_00863" }, { "type": "cds", "sequence": "contig_1", "start": 473752, "stop": 474069, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGCAGGAGTTCTTTTATTACAAATATGGTTAAAATGCAAATATGCTAATGTTCAGTTTGGTGAACATGGTTTTAATGGAGATGAATTTTATTTAGATTTTTATATAAATGAGAATTTTTCTACAAAACAGTTTGCAAAAATAGAATCTGATTTAAACTCTCTTTCTAGTAAACTAGAAGGGATTTCTCAAAAATTTGTTTCTTTAGATGAAGCATTAAGTTTTTTTGAAAATGATCAGTTTACAAAAAACTTATTAAAAAAAAGCAACTTAAACAAATTTAAAATCACCTTTTTTGAAAATAAACATTTTTGA", "aa": "MLAGVLLLQIWLKCKYANVQFGEHGFNGDEFYLDFYINENFSTKQFAKIESDLNSLSSKLEGISQKFVSLDEALSFFENDQFTKNLLKKSNLNKFKITFFENKHF", "aa_hexdigest": "b4b0e43b237b64ae68489a9309012765", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12294.846999999982, "isoelectric_point": 6.08395481109619 }, "id": "JPCNHPFPBM_865", "locus": "GCA000292505_00864" }, { "type": "cds", "sequence": "contig_1", "start": 474141, "stop": 474290, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MG376", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75224", "UniRef:UniRef90_P47616" ], "nt": "TTGTTTGGTGAACGAAGTTTTACTGATTTTTTTGTTTTTTATGCAAATGGTCAATTAGCATTTGAACTTGAAGCATATACTCAAAAATTTAAAACAATTAGCATCGAAGGTATTCTGCGCACTTACCTAGAAAAACGCAGTGGAATTTGA", "aa": "MFGERSFTDFFVFYANGQLAFELEAYTQKFKTISIEGILRTYLEKRSGI", "aa_hexdigest": "447289fe1f00fc36f0ecc097a52e7c81", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 474069, "stop": 474383, "observations": { "mutation": [ 474143, 474338 ], "stop": [ 474143, 474338 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47616", "query_cov": 1.0, "subject_cov": 0.47115384615384615, "identity": 0.98, "score": 96.7, "evalue": 5.41e-25, "gene_start": 1, "gene_end": 49, "reference_start": 32, "reference_end": 80, "reference_sequence": "MLNCVFLEGEIESTKWSHKKTGFLVTIKQKRLFGERSFTDFFVFYANGQLAFELEAYTQKFKTISIEGILRTYLEKRSGIWKTTIEVVKIMKPNSKVMIDYQES" }, "paralog": false, "identity": 0.9807692307692307, "score": 199.0, "evalue": 4.55e-68, "description": "Internal stop codon at 474143, 474338. Nonsense mutation around 474143, 474338.", "psc": { "uniref90_id": "UniRef90_P47616", "product": "Uncharacterized protein MG376", "uniref50_id": "UniRef50_P75224", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47616", "UniRef:UniRef50_P75224" ] }, "pscc": { "uniref50_id": "UniRef50_P75224", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75224" ], "product": "Uncharacterized protein MG376 homolog" } }, "id": "JPCNHPFPBM_866", "locus": "GCA000292505_00865" }, { "type": "cds", "sequence": "contig_1", "start": 474389, "stop": 474547, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTATGAAAAAACTAATCAGAATGTTAGAGAATATCTTGATGATAAACAAAAGTTTGAAGGTATTAGAACTGCATTAATAGAAGAAAGAATGGTTTTAGAAACAATTAACCACTTTAAATTTCATTTCAACTTAACAGGTCAATTACCTAGCTAA", "aa": "MYYEKTNQNVREYLDDKQKFEGIRTALIEERMVLETINHFKFHFNLTGQLPS", "aa_hexdigest": "79e6bb9022c550ad92e75000777a3f5d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6337.1151999999975, "isoelectric_point": 6.043030738830566 }, "id": "JPCNHPFPBM_867", "locus": "GCA000292505_00866" }, { "type": "cds", "sequence": "contig_1", "start": 474590, "stop": 474970, "strand": "-", "frame": 3, "gene": null, "product": "MPN555 family protein chaperone", "db_xrefs": [ "GO:0006457", "GO:0015031", "SO:0001217", "UniRef:UniRef50_P75223", "UniRef:UniRef90_P47617" ], "nt": "ATGGCTACTAATCTTAAATCTATTGCAAAGTTGCAAAAATCTATTCAGTATGATAAGGTAATTGAAGTTGATCGTATTTTTGCAGATCCTGCTTTTATTGAGCAACATCGGCAAAAAATTTTAGCTTCTTTTAAAGATGCTAAGGAAAGTGCTCTTTACCACGAACTAACTCATATTGTTATTAAAGATAATTTGTTTTCAGCAGCAATGAATGAAATTGTTAGTTATTTTGAATTTCAAATTAACCCTGAAGAGTTAAAAAATGTTGTTGAAGGCTTGAAAAGAGATGTTGTTAAAGATGCTGATGAAAAAACAATTCAATCAATAGCTGAAAAAATCATTAAAAAAGCTTTAGTATTTAATTTCTTACAAAAGGAATGA", "aa": "MATNLKSIAKLQKSIQYDKVIEVDRIFADPAFIEQHRQKILASFKDAKESALYHELTHIVIKDNLFSAAMNEIVSYFEFQINPEELKNVVEGLKRDVVKDADEKTIQSIAEKIIKKALVFNFLQKE", "aa_hexdigest": "dfb32580606d2cb162025014b8726dbc", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 474389, "stop": 474970, "observations": { "mutation": [ 474592 ], "stop": [ 474592 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47617", "query_cov": 1.0, "subject_cov": 0.6528497409326425, "identity": 0.9840000000000001, "score": 236.0, "evalue": 7.99e-78, "gene_start": 1, "gene_end": 126, "reference_start": 1, "reference_end": 126, "reference_sequence": "MATNLKSIAKLQKPIQYDKVIEVDRIFADPAFIEQHRQRILASFKDAKESALYHELTHIVIKDNLFSAAMNEIVSYFEFQINPEELKNVVEGLKRDVVKDADEKTIQSIAEKIIKKALVFNFLQKEWKVEVSDDIVKRVISLYYEKTNQNVREYLDDKQKFEGIRTALIEERMVLETINHFKFHFNLTGQLPS" }, "paralog": false, "identity": 0.9844559585492227, "score": 368.0, "evalue": 2.92e-132, "description": "Internal stop codon at 474592. Nonsense mutation around 474592.", "psc": { "uniref90_id": "UniRef90_P47617", "product": "MPN555 family protein chaperone", "uniref50_id": "UniRef50_P75223", "go_ids": [ "GO:0006457", "GO:0015031" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47617", "UniRef:UniRef50_P75223", "GO:0006457", "GO:0015031" ] }, "pscc": { "uniref50_id": "UniRef50_P75223", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75223" ], "product": "Uncharacterized protein MG377 homolog" } }, "id": "JPCNHPFPBM_868", "locus": "GCA000292505_00867" }, { "type": "cds", "sequence": "contig_1", "start": 475017, "stop": 475457, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05746.21" ], "nt": "ATGTTATCTCAAAATAATGGCACAATTATCAAAATTGATTTAGATATAGCTAATTTGCAAAACTCTGCTAATCCAGTTTATTATGTTCAATATGCGTTTGCAAGAATGAATAGTATTCTTAGAATTGCAAATTCTGATCAATTAAAAGAAATTACTGATTGCAGTCTTTTGATTAATGAAAAAGAGATATCACTTTTAAATCAACTTGTCTATTATCCTTTTATGTTGCAAAAAGCTATGGAAACAGGCGAATTGCACTTATTAACTAACTTTTTATATGAAACTGCTAGTTTATTTCATTCCTGGTATAAAGTTTGCAAAATTAATGATGATAAAAATTCACTTTTATCAGCACAAAGACTTGCTTTATTGAGATCATTACAATTTATAGTTAAACAAATCCTTGATGTTTTGAAGATTTCAACACCACAACAAATGTAA", "aa": "MLSQNNGTIIKIDLDIANLQNSANPVYYVQYAFARMNSILRIANSDQLKEITDCSLLINEKEISLLNQLVYYPFMLQKAMETGELHLLTNFLYETASLFHSWYKVCKINDDKNSLLSAQRLALLRSLQFIVKQILDVLKISTPQQM", "aa_hexdigest": "87c6248af5377605c2de863a68a2612b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05746.21", "name": "DALR_1", "length": 118, "aa_cov": 0.8082191780821918, "hmm_cov": 0.9915254237288136, "evalue": 1.4467122916435131e-32, "score": 107.3184814453125, "start": 29, "stop": 146 } ], "seq_stats": { "molecular_weight": 16834.410199999988, "isoelectric_point": 6.714128684997559 }, "id": "JPCNHPFPBM_869", "locus": "GCA000292505_00868" }, { "type": "cds", "sequence": "contig_1", "start": 475461, "stop": 475637, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATTGTGCATTTGATGCTTTAAAAAATACCACTAAAACTTCTTTAAAAATTTTTGCATTACAACTGGTTACTCTCTATAAAAATAAAGAGCTAGTACGTTTGTCAAAACGTGCTGGAAATGTAATCACAATTGAAACAATGCTTTCAATGATTAGTGAAGATGCTGCTAGATGA", "aa": "MYCAFDALKNTTKTSLKIFALQLVTLYKNKELVRLSKRAGNVITIETMLSMISEDAAR", "aa_hexdigest": "5cf8facb3ea03ab024ad0e3e0b54929d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6554.7204, "isoelectric_point": 9.57898654937744 }, "id": "JPCNHPFPBM_870", "locus": "GCA000292505_00869" }, { "type": "cds", "sequence": "contig_1", "start": 475902, "stop": 476354, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAAAAACACCTTGCTTTACTAAGAAAAATATAAAGATTTTAATTGAATCTGTATCAGCAAATCCTACCGGAAGGATCCATTTAGGTCATGTGAGAATAGCTTTTTTTGGTGATGTTTTAAACAATTTAGCCAAGCTGTTGGGTTATACAACAGTCTGTGAATATTGGGTAAATGATTATGGACAACAAGCACGAGTTTTTAGCTTTAGTGTTTATCAAAGTTTGCAGTTAAAAAAAAATATTGCTATCCAGCAACATCCTGATGGATATAGTGGAATAGTAATAGATAAAATTGCTAGTGAAATTGAAAATTTTCCAGTTGATAATTTAAATTTTGAAGAGTTTTGTAAAACATCATTCTTAGATCATTTTTTAGTTAATTGCACCCAAAAAGTTTTGTCTTTAATTAAAAGTGATTTGAATAAAATCCATGTTTTTATTGATAGTTGA", "aa": "MIKTPCFTKKNIKILIESVSANPTGRIHLGHVRIAFFGDVLNNLAKLLGYTTVCEYWVNDYGQQARVFSFSVYQSLQLKKNIAIQQHPDGYSGIVIDKIASEIENFPVDNLNFEEFCKTSFLDHFLVNCTQKVLSLIKSDLNKIHVFIDS", "aa_hexdigest": "7816ca1076a1f28b8e3604d69c9ac6ab", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17073.515300000006, "isoelectric_point": 7.690679359436037 }, "id": "JPCNHPFPBM_871", "locus": "GCA000292505_00870" }, { "type": "cds", "sequence": "contig_1", "start": 476451, "stop": 476630, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTTATCATCAATGATTTAAAAGAATGCATTAGCGCTTTAAAGCTTAAATTTGATGACCAAAAGGAACTTGTTAAACTAGTTAAAAATAATAGTTTTAATGGTTTTTCTTCAACTATTATTTTCCAACTAAAAAGTGAAAATCATAAAAAAATTGCAGATAGTATTGTTGAGTGA", "aa": "MFFIINDLKECISALKLKFDDQKELVKLVKNNSFNGFSSTIIFQLKSENHKKIADSIVE", "aa_hexdigest": "ac98275191574655d733bb35ae25a931", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6820.880599999999, "isoelectric_point": 7.868640327453613 }, "id": "JPCNHPFPBM_872", "locus": "GCA000292505_00871" }, { "type": "cds", "sequence": "contig_1", "start": 476659, "stop": 477963, "strand": "+", "frame": 1, "gene": "mnmG", "product": "tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A654IKD5" ], "nt": "ATGTCTTTTATCATAACAGTAATAGGTGCTGGGCATGCTGGATTGGAAGCCGCTTTCATTGTAAGCAAATTCAACATCAAAGTAAACCTTTTAGTTCTTGATATAAATCATTTAGGTTCTTGTCCATGTAATCCTTCAATTGGTGGACCTGCTAAGGGAATTGTTACTAGGGAAATTGATGTTTTAGGAGGTATGCAAGCAATTGCTGCTGATAACAATGCCTTACAATATAAATTACTAAATAGTTCAAAAGGACCTGCTGTGCAAGCTATCAGAGCACAAATTGACAAAATAGGTTATAAAAACTGGTTTCAAAGTCAAGTTAAATTAAATAAAAACATTAATCTAATTCAATCTGAAGCAATCAATTTAATTGTTAGAAATGAAAAAATAAAAGGCGTTATTTTAAAAGACGGAAGTGAACTTTTAAGTGATGCGGTTATTATCACTACCGGAACGTACCTAAGATCAAAAACATACTGTGGTAATACAGTTAAAAATCAAGGACCTGATCAATCTAAAAATAGTGAAAAATTAAGCACAAACTTAATTAACAGAGGTTTTAAAACAATTCGTTTAAAAACAGGAACTCCGCCAAGAATTTTAAAAACTTCACTTGACTATAATCAAATGGAATTAGAAATTAATAATAATCAAAACCTTGCTTTTAGTACTACAAATAAAAATTTCTTACCACTTGAAAAACAAATACCTTGTTACTTAGTTCATACCAATCAAAAAATTCACGATCTAATCCTTAAAAACTTAAAAAAATCTGCAATGTTTAATGGTAGTATTTCAGCACAAGGACCACTTTATTGTCCAAGCATTGAAGACAAAGTTTTTAAGTTCTCTCAAAAACCTCGTCACCAAATTTTTGTAGAACCTGAATCATTGAGTCTAGATACTATTTATTTAGCAGGATTATCAACTTCTTTTACACCAGAAATTCAAAAAGAAATCATCCAGCTTTTACCTGGTTTTCAAAATGCAGAAATTAAAAAGTTTGGTTACGCTATTGAATATGATGCTTTTCTATCTAATCAACTAAAACCAACACTTGAAACGAAGTTAATAGAAAACTTGTATTTTGCTGGACAAATTAATGGCACTAGCGGTTATGAAGAAGCTGCTGGTCAAGGTTTGATGGCAGGAATTAATGCTGCTTTAAAATTATTAAAAAAACCACCATTTATTTTGCAACGTAATGAGGCTTATATTGGGGTTATGATTAATGATTTAGTTACTAAAACAATCAGTGATCCATACCGTTTGTTAACATCCAGAGCAGAATATAGACTATGA", "aa": "MSFIITVIGAGHAGLEAAFIVSKFNIKVNLLVLDINHLGSCPCNPSIGGPAKGIVTREIDVLGGMQAIAADNNALQYKLLNSSKGPAVQAIRAQIDKIGYKNWFQSQVKLNKNINLIQSEAINLIVRNEKIKGVILKDGSELLSDAVIITTGTYLRSKTYCGNTVKNQGPDQSKNSEKLSTNLINRGFKTIRLKTGTPPRILKTSLDYNQMELEINNNQNLAFSTTNKNFLPLEKQIPCYLVHTNQKIHDLILKNLKKSAMFNGSISAQGPLYCPSIEDKVFKFSQKPRHQIFVEPESLSLDTIYLAGLSTSFTPEIQKEIIQLLPGFQNAEIKKFGYAIEYDAFLSNQLKPTLETKLIENLYFAGQINGTSGYEEAAGQGLMAGINAALKLLKKPPFILQRNEAYIGVMINDLVTKTISDPYRLLTSRAEYRL", "aa_hexdigest": "c2091ad575b32d3d0cf2e229f8e9ec2d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030D362B2", "query_cov": 0.9423963133640553, "subject_cov": 0.9975609756097561, "identity": 0.531, "score": 435.0, "evalue": 9.68e-148, "valid": false, "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "uniref50_id": "UniRef50_A0A654IKD5", "kegg_orthology_id": "K03495", "go_ids": [ "GO:0002098", "GO:0008033" ] }, "pscc": { "uniref50_id": "UniRef50_A0A654IKD5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A654IKD5" ], "product": "tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG" }, "genes": [ "mnmG" ], "id": "JPCNHPFPBM_873", "locus": "GCA000292505_00872" }, { "type": "cds", "sequence": "contig_1", "start": 477964, "stop": 478497, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF21680.2", "PFAM:PF13932.14" ], "nt": "TTGAGAAATGACAATGTTCAAGAACGGCTCATTAAAAAAAGCTTTGAACTTGGTTTAACAGATAAAAAAACATATGAATTGTTCCTTAAAAAGGAAAAGAAAAAACAGGAATTAATTTCATTTTTAAAAAACACTCAAGTAGGCAAGGTTAAAGCATTGAAATTCACTAATAAAAATACCGCTCAATCACTTTATGACTTCAACAAACGAAGTGAAATAAATTTAGATAAATTGATCAAAGATCTTCCTGAAAAATACCAATTAGATTCAGAAACACTTAAACAAATTGAAATTGAAATTAAATATGAGGGTTACATAAAGAAAAATGAAAAGTATTTTAAGGGTTTAGATAAATTAAGCAAAATTAAAATTCCTCATACTTTTGATTACCATAAGGTTAAGAATTTAGCTAGTGAAGCTATTTTTAAACTATCTAACTTTAAGCCTAGTAATTTAGCAATTGCAAGTCAAATAGCTGGAGTGAACTTTAATGACATTATAGCCATAAAACATTTTTTAAAAACTTATGAATAA", "aa": "MRNDNVQERLIKKSFELGLTDKKTYELFLKKEKKKQELISFLKNTQVGKVKALKFTNKNTAQSLYDFNKRSEINLDKLIKDLPEKYQLDSETLKQIEIEIKYEGYIKKNEKYFKGLDKLSKIKIPHTFDYHKVKNLASEAIFKLSNFKPSNLAIASQIAGVNFNDIIAIKHFLKTYE", "aa_hexdigest": "9d59526094681e52fd896cf9b3f67b0f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF21680.2", "name": "GIDA_C_1st", "length": 94, "aa_cov": 0.4915254237288136, "hmm_cov": 1.0, "evalue": 6.152190433313451e-19, "score": 63.734519958496094, "start": 23, "stop": 109 }, { "id": "PF13932.14", "name": "SAM_GIDA_C", "length": 53, "aa_cov": 0.2994350282485876, "hmm_cov": 1.0, "evalue": 2.6190540608961974e-16, "score": 54.344112396240234, "start": 117, "stop": 169 } ], "seq_stats": { "molecular_weight": 20800.948500000013, "isoelectric_point": 9.61631374359131 }, "id": "JPCNHPFPBM_874", "locus": "GCA000292505_00873" }, { "type": "cds", "sequence": "contig_1", "start": 478490, "stop": 479068, "strand": "+", "frame": 2, "gene": "rsmG", "product": "16S rRNA (guanine(527)-N(7))-methyltransferase RsmG", "db_xrefs": [ "COG:COG0357", "COG:J", "EC:2.1.1.-", "EC:2.1.1.170", "GO:0005829", "GO:0008168", "GO:0031167", "GO:0046118", "GO:0070043", "KEGG:K03501", "RefSeq:WP_009885942.1", "SO:0001217", "UniParc:UPI000012B473", "UniRef:UniRef100_P47620", "UniRef:UniRef50_P75220", "UniRef:UniRef90_P47620" ], "nt": "ATGAATAATGCTAATTTTGAAAAATATGTTGATTTAGTTTTTGAAGCAAACAAAAATTTCAACTTAACAGGATTTAAAACAAAAGAAGCTATTTATCAGAATTTAGTTATAGAAATATTGACATTATTTAAAGGATATGAAAAATTTTTTATTGACAAAACTGTAGCAGACTTGGGAAGTGGAAATGGTTCGCCTGGGATAATATTAAAACTGTTATTTCAAAAAATAAAAAAGTTAGTTTTAATTGATAGTAAACACAAAAAAATTAGCTTTTTAAATAAATTAACTAAGCAACTAAATCTGGAGAAAACTGTTGCAATTTGTGAACGAATTGAAGTACATAAAAATCACTATGATGTTATCTGTTCTCGTGGTCTAAGTACGATTATTAAAGTTAATGATTTAGCATTTTCCTTGCTTAACTCAAAAGGTATTATTTTTCATATAAAACAAAGCTTAGACCAATACATTGAATTTGAAAAATCAAATCAGAAGAATCAATTTAACTTGTTATTTATAAAGCACTTTACTAGTCAGAATAAAAAACTAATTTTGATAGCTTTACAAAAAAATGATTAA", "aa": "MNNANFEKYVDLVFEANKNFNLTGFKTKEAIYQNLVIEILTLFKGYEKFFIDKTVADLGSGNGSPGIILKLLFQKIKKLVLIDSKHKKISFLNKLTKQLNLEKTVAICERIEVHKNHYDVICSRGLSTIIKVNDLAFSLLNSKGIIFHIKQSLDQYIEFEKSNQKNQFNLLFIKHFTSQNKKLILIALQKND", "aa_hexdigest": "a4edbded6dab27c0c17cc18c7eaea59b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012B473", "ncbi_nrp_id": "WP_009885942.1", "uniref100_id": "UniRef100_P47620", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012B473", "RefSeq:WP_009885942.1", "UniRef:UniRef100_P47620" ] }, "ips": { "uniref100_id": "UniRef100_P47620", "uniref90_id": "UniRef90_P47620" }, "psc": { "uniref90_id": "UniRef90_P47620", "gene": "rsmG", "product": "16S rRNA (guanine(527)-N(7))-methyltransferase RsmG", "ec_ids": [ "2.1.1.-", "2.1.1.170" ], "uniref50_id": "UniRef50_P75220", "cog_id": "COG0357", "cog_category": "J", "kegg_orthology_id": "K03501", "go_ids": [ "GO:0005829", "GO:0008168", "GO:0031167", "GO:0046118", "GO:0070043" ] }, "pscc": { "uniref50_id": "UniRef50_P75220", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75220" ], "product": "Ribosomal RNA small subunit methyltransferase G" }, "genes": [ "rsmG" ], "id": "JPCNHPFPBM_875", "locus": "GCA000292505_00874" }, { "type": "cds", "sequence": "contig_1", "start": 479373, "stop": 479717, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MG381", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75219", "UniRef:UniRef90_P47621" ], "nt": "ATGTTGATTGATGTTCAACATCATAAAAAGTTACAAAAATGTTTATTAAGAGAAATTCATCTTTTAGTTTGTTTAATTAATAGCAATCAATTTAGTGATGAATTAATGCAAATTATTGAGTGGTATCAGTATTTAAAAAAACACTCTAGTAAACTTTTTGTTATTACTGCAAGTAGTGATAAAAAACCAGTAATAGAGCCAACTATAAATGAATATAAAGCTATTTTTGGAGAGTATTTATCTTCATTTCACCTAGATCTAAAAAATAATCAAAGTAATGATTTATTCCAGAAATTACTTGATCAGATCAAAATAAAAGCTACCTCAAAAACTAGTTTGAGATAG", "aa": "MLIDVQHHKKLQKCLLREIHLLVCLINSNQFSDELMQIIEWYQYLKKHSSKLFVITASSDKKPVIEPTINEYKAIFGEYLSSFHLDLKNNQSNDLFQKLLDQIKIKATSKTSLR", "aa_hexdigest": "deb57aafa407fa60704d744034546606", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 479073, "stop": 479717, "observations": { "mutation": [ 479076 ], "stop": [ 479076 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47621", "query_cov": 1.0, "subject_cov": 0.5229357798165137, "identity": 1.0, "score": 224.0, "evalue": 8.23e-73, "gene_start": 1, "gene_end": 114, "reference_start": 105, "reference_end": 218, "reference_sequence": "MIKLGWASFSNTEFAQDIFIKFANQFYKQDDAGTILFELKKTLGVNQLDEIEKNKKVIIVNQFQNSLGKFLLFNKENTKRINSLANEHISSFLKKIFRLSGFKDMLIDVQHHKKLQKCLLREIHLLVCLINSNQFSDELMQIIEWYQYLKKHSSKLFVITASSDKKPVIEPTINEYKAIFGEYLSSFHLDLKNNQSNDLFQKLLDQIKIKATSKTSLR" }, "paralog": false, "identity": 0.9953271028037384, "score": 419.0, "evalue": 3.68e-152, "description": "Internal stop codon at 479076. Nonsense mutation around 479076.", "psc": { "uniref90_id": "UniRef90_P47621", "product": "Uncharacterized protein MG381", "uniref50_id": "UniRef50_P75219", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47621", "UniRef:UniRef50_P75219" ] }, "pscc": { "uniref50_id": "UniRef50_P75219", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75219" ], "product": "Uncharacterized protein MG381 homolog" } }, "id": "JPCNHPFPBM_876", "locus": "GCA000292505_00875" }, { "type": "tRNA", "sequence": "contig_1", "start": 479731, "stop": 479806, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(tct)", "amino_acid": "Arg", "anti_codon": "tct", "score": 73.5, "nt": "GCGTCGGTAGCTCAGTGGATAGAGTACACGCCTTCTAAGCGTGTTGTCGTGGGTTCAAATCCCTCCCGATGCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 479771, 479773 ], "id": "JPCNHPFPBM_17", "locus": "GCA000292505_00876" }, { "type": "cds", "sequence": "contig_1", "start": 480182, "stop": 480385, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00485.24" ], "nt": "ATGGATGAAAAAGGGATTTTAGTTGCAATTAGTGGTGGTAGTTGCTCAGGAAAAACTACTGTTGCTGAAATGATTTATCAACTTTTAAGTAAAAAATTAAAAGTTGCGATCATCTGTCAAGATAACTATTACAAGTCCTATAAAAATAAGCCATTATTAAAAAGAAAAACAATAAACTTTGATCATCCTGATGCTTTTGATTGA", "aa": "MDEKGILVAISGGSCSGKTTVAEMIYQLLSKKLKVAIICQDNYYKSYKNKPLLKRKTINFDHPDAFD", "aa_hexdigest": "a0000b9afdf58a61839e54b3f3972c66", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00485.24", "name": "PRK", "length": 69, "aa_cov": 0.8955223880597015, "hmm_cov": 1.0, "evalue": 8.966480974195929e-07, "score": 23.982669830322266, "start": 8, "stop": 67 } ], "seq_stats": { "molecular_weight": 7541.7423, "isoelectric_point": 9.171868705749514 }, "id": "JPCNHPFPBM_877", "locus": "GCA000292505_00877" }, { "type": "cds", "sequence": "contig_1", "start": 480820, "stop": 480975, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCATTACTTATAAAGAACTTGATCAATATTTAAAAGGTGATTTAATACTTAGTTCAGAAAAGCAAAAAATTGTTTTAGATTTGAAAGCAAAAGCAGAGCATAAACATAATTCACCTTTGAAATTTAAACATCTCTATAATTTCCAGAACTAA", "aa": "MGITYKELDQYLKGDLILSSEKQKIVLDLKAKAEHKHNSPLKFKHLYNFQN", "aa_hexdigest": "9fda724ba694131d3d3ba14dab2e308e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6016.9207, "isoelectric_point": 9.302675056457517 }, "id": "JPCNHPFPBM_878", "locus": "GCA000292505_00878" }, { "type": "cds", "sequence": "contig_1", "start": 481123, "stop": 481566, "strand": "-", "frame": 1, "gene": "nadE", "product": "NH(3)-dependent NAD(+) synthetase", "db_xrefs": [ "COG:COG0171", "COG:H", "EC:6.3.1.5", "GO:0003952", "GO:0004359", "GO:0005524", "GO:0005737", "GO:0008795", "GO:0009435", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47623", "UniRef:UniRef90_P47623" ], "nt": "ATGACTAATTTAATTAAATATTTAAAAGAACTCCAAAACTGGCTGTTTGATTATGTAAAAAAATCTAAAGCTAAAGGTGTTATTTTTGGCTTATCTGGAGGAATTGATTCAGCAGTTGTTGCTGCTATTGCTAAAGAAACTTTTGGTTTTGAAAACCATTTAGCTTTAATAATGCATATTAATAATTCAAAACTTGATTTTCAAGCAACTAGTGAACTTGTTAAAAAAATGCAATTTAATAGTATTAACATTGAACTGGAAGAGAGTTTCAATCTGTTAGTAAAAACCCTTGGAATAGATCCAAAAAAAGATTTTTTAACAGCTGGTAACATTAAAGCACGTTTACGGATGATAACTTTATATGCTTATGCTCAAAAACACAACTTCTTAGTTTTAGGTACTGGTAATTTTGTAGAGTATACACTTGGTTATTTCACAAAATGA", "aa": "MTNLIKYLKELQNWLFDYVKKSKAKGVIFGLSGGIDSAVVAAIAKETFGFENHLALIMHINNSKLDFQATSELVKKMQFNSINIELEESFNLLVKTLGIDPKKDFLTAGNIKARLRMITLYAYAQKHNFLVLGTGNFVEYTLGYFTK", "aa_hexdigest": "b32fde3d78a1939af3058617b114fb9f", "start_type": "ATG", "rbs_motif": null, "genes": [ "nadE" ], "truncated": "5-prime", "pseudogene": { "start": 480820, "stop": 481566, "observations": { "mutation": [ 481125 ], "stop": [ 481125 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47623", "query_cov": 1.0, "subject_cov": 0.592741935483871, "identity": 1.0, "score": 290.0, "evalue": 8.23e-98, "gene_start": 1, "gene_end": 147, "reference_start": 1, "reference_end": 147, "reference_sequence": "MTNLIKYLKELQNWLFDYVKKSKAKGVIFGLSGGIDSAVVAAIAKETFGFENHLALIMHINNSKLDFQATSELVKKMQFNSINIELEESFNLLVKTLGIDPKKDFLTAGNIKARLRMITLYAYAQKHNFLVLGTGNFVEYTLGYFTKWGDGACDIAPLAWLLKEDVYKLAKHFNIPEIVITRAPTASLFEGQTDETEMGITYKELDQYLKGDLILSSEKQKIVLDLKAKAEHKHNSPLKFKHLYNFQN" }, "paralog": false, "identity": 0.9959677419354839, "score": 491.0, "evalue": 1.31e-179, "description": "Internal stop codon at 481125. Nonsense mutation around 481125.", "psc": { "uniref90_id": "UniRef90_P47623", "gene": "nadE", "product": "NH(3)-dependent NAD(+) synthetase", "ec_ids": [ "6.3.1.5" ], "uniref50_id": "UniRef50_P47623", "cog_id": "COG0171", "cog_category": "H", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0004359", "GO:0046872", "GO:0003952", "GO:0008795", "GO:0009435" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47623", "EC:6.3.1.5", "UniRef:UniRef50_P47623", "COG:COG0171", "COG:H", "GO:0005737", "GO:0005524", "GO:0004359", "GO:0046872", "GO:0003952", "GO:0008795", "GO:0009435" ] }, "pscc": { "uniref50_id": "UniRef50_P47623", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47623" ], "product": "NH(3)-dependent NAD(+) synthetase" } }, "id": "JPCNHPFPBM_879", "locus": "GCA000292505_00879" }, { "type": "cds", "sequence": "contig_1", "start": 481742, "stop": 482722, "strand": "-", "frame": 3, "gene": null, "product": "GTPase Obg", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q2S3C0" ], "nt": "TTGCAAGCTGATCATAACTGCGATTCACTTTTTTTCTTGAAGAATAAAAAACACCTGTTTGCAGAAGATGGACAAAATGGTAAACCAGATTTAGCACATGGTAAAAATGGTTCAGATCTTTTAATTAAAGTGCCAATAGGAACAACAGTTAAAAACTTAGAAAATAATAGTGTATTGGTGGATTTTGTTCATGATAAACAGAGTTTTATTTTGTGTTTTGGCGGAAAAGGCGGAAAAGGAAATGCTGCTTTTAAAAGCCCAATTATGCGTGCTCCTAATCTATATGAAAATGGTGATAAAGGAGAGATTTTAAACGTTAGTTTGGAAGTTAAATATTTAGCTAATGTAGGAATTGTTGGTTTTCCAAATTCAGGTAAATCAACACTAATCTCCAAACTTTCAAATGCCAAACCTAAAATTGCAAACTATCGCTTTACTACTTTAATACCCGTACTTGGTGTAGTTAAATATCAAAATAATAGTTTAGTTTTTGCTGATATTCCTGGTTTGATTGAAAATGCTAGTGAAGGAAGTGGGTTAGGACATGATTTTTTACGGCATATAGAGAGGTGTGAAATATTAATTCATTTAATTTCACTTGATCCTGTAGATAATGATGATCCTTGCAAAGCATATTTACAAATAATGGATGAACTTTCAAAATATTCTCCCCTTCTAGTTAAAAAAAAGATGCTTGTTGTAGCTAATAAAATTGATGTTAATGAAGGTGAGAAACGGTTTAAAAAATTAGAAAAATTTCTTCAAAAAAAATCTATTTCTGTATTAAAAATTTCTGCTTTAAAGAAAGAATTAGGCAATTTACTTGATAGAGTTTTTGAACTTTACAATAAAACAATTTCCCAATTTGGAGCTAATAAGTTTAGTTTGCCAATGGAACTTGAGAAGCATTATGTTTTTCAAAATACCAATGAAAATAATAACGACCCTTTAAATATTGAAAAAGATAGTTTAAACAGATGA", "aa": "MQADHNCDSLFFLKNKKHLFAEDGQNGKPDLAHGKNGSDLLIKVPIGTTVKNLENNSVLVDFVHDKQSFILCFGGKGGKGNAAFKSPIMRAPNLYENGDKGEILNVSLEVKYLANVGIVGFPNSGKSTLISKLSNAKPKIANYRFTTLIPVLGVVKYQNNSLVFADIPGLIENASEGSGLGHDFLRHIERCEILIHLISLDPVDNDDPCKAYLQIMDELSKYSPLLVKKKMLVVANKIDVNEGEKRFKKLEKFLQKKSISVLKISALKKELGNLLDRVFELYNKTISQFGANKFSLPMELEKHYVFQNTNENNNDPLNIEKDSLNR", "aa_hexdigest": "7b8985391b5fb4e4d156ae57c3e0421d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A432RYP9", "query_cov": 0.8773006134969326, "subject_cov": 0.8461538461538461, "identity": 0.5, "score": 274.0, "evalue": 3.27e-87, "valid": false, "gene": "obgE", "product": "GTPase ObgE", "uniref50_id": "UniRef50_Q2S3C0", "go_ids": [ "GO:0003924" ] }, "pscc": { "uniref50_id": "UniRef50_Q2S3C0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q2S3C0" ], "product": "GTPase Obg" }, "genes": [], "id": "JPCNHPFPBM_880", "locus": "GCA000292505_00880" }, { "type": "cds", "sequence": "contig_1", "start": 482860, "stop": 483036, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTAACTTTTTTTTAATCAGATCAAAATATGCAATAATAATTGCTGAAAATCTCTTAAAGACAGGTAAATCTAAAACTAAAAATCGACAAAAGTTGCAACAAATTCAAAGTGATCTAGAAAGAAAAAAATCTGCAGAAATCGTTTTTAAAATAAAGAAAAAGAAACCAAAATAA", "aa": "MTNFFLIRSKYAIIIAENLLKTGKSKTKNRQKLQQIQSDLERKKSAEIVFKIKKKKPK", "aa_hexdigest": "407bc0392a647678fedd91e7da762425", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6850.172500000002, "isoelectric_point": 10.582372474670407 }, "id": "JPCNHPFPBM_881", "locus": "GCA000292505_00881" }, { "type": "cds", "sequence": "contig_1", "start": 483561, "stop": 484007, "strand": "-", "frame": 2, "gene": "ugpQ", "product": "putative protein MG385", "db_xrefs": [ "COG:COG0584", "COG:I", "GO:0006629", "GO:0008081", "SO:0001217", "UniRef:UniRef50_P47625", "UniRef:UniRef90_P47625" ], "nt": "TTGAATTTAAACCAAATTAATTTAGTTGATCTTAAAAAGGTTAACCTTGCAAGTGAATTTAAGCTTAAGGTAACTTCACAACAAATTCAAACTTTAAAGGAAGTTTTAACGCAATTTATTCAGCCCTTTAAATATTTGCTGATTCATATTAAAGATGATAAAGAAAATAACAATTCATTATTAGAACAGTTGAATTTGCTTTGCAAAGATTTTGTCTTAGCTAAAGAAAAAATGATTTTGTTAAGTAGTAATTTTCATATCATTAAGTTAATAAATGAGACTTTCAAAGGCTTTAAAACCGGTTTTGTAATTGCAAACAAAAAAGCTTTATTAGTAGCTTCTAGAGACGAATTAATGAGATATTGCCGTTTTTTAGTGCCAAACGAGAGTTTTTATCATAAGAACTGCAAAGAAATTCAAAACCTGGGATTACCAGTTATTCTTTGA", "aa": "MNLNQINLVDLKKVNLASEFKLKVTSQQIQTLKEVLTQFIQPFKYLLIHIKDDKENNNSLLEQLNLLCKDFVLAKEKMILLSSNFHIIKLINETFKGFKTGFVIANKKALLVASRDELMRYCRFLVPNESFYHKNCKEIQNLGLPVIL", "aa_hexdigest": "5e07803e801b973a2b11c61d93e0224b", "start_type": "TTG", "rbs_motif": null, "genes": [ "ugpQ" ], "truncated": "both", "pseudogene": { "start": 483480, "stop": 484190, "observations": { "mutation": [ 483563, 484085 ], "stop": [ 483563, 484085 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47625", "query_cov": 1.0, "subject_cov": 0.6271186440677966, "identity": 0.993, "score": 287.0, "evalue": 9.43e-97, "gene_start": 1, "gene_end": 148, "reference_start": 62, "reference_end": 209, "reference_sequence": "MRKQFLIAYRGYSSIAPENTKLAFQAAQVFDFDGIWIDIQLTKDKQIVVTHKENFKVSNKNLNLNQINLVDLKKVNLASEFKLKVTSQQIQTLKEVLTQFIQPFKYLLIHIKDDKENNNSLLEQLNLLCKDFVLAKEKMILLSSNFHIIKLINETFKGFKTGFVIANKKALLVASRDELMRYCRFLVPNESFYHKNCKEIQNLGLPVILWVIKGLLRYQFYENDRFVKFQITAQIY" }, "paralog": false, "identity": 0.9915254237288136, "score": 457.0, "evalue": 3.13e-166, "description": "Internal stop codon at 483563, 484085. Nonsense mutation around 483563, 484085.", "psc": { "uniref90_id": "UniRef90_P47625", "gene": "UgpQ", "product": "Uncharacterized protein MG385", "uniref50_id": "UniRef50_P47625", "cog_id": "COG0584", "cog_category": "I", "go_ids": [ "GO:0008081", "GO:0006629" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47625", "UniRef:UniRef50_P47625", "COG:COG0584", "COG:I", "GO:0008081", "GO:0006629" ] }, "pscc": { "uniref50_id": "UniRef50_P47625", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47625" ], "product": "Uncharacterized protein MG385" } }, "id": "JPCNHPFPBM_882", "locus": "GCA000292505_00882" }, { "type": "cds", "sequence": "contig_1", "start": 484083, "stop": 484190, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGAAAACAGTTTTTAATAGCTTATCGCGGTTATAGTTCTATTGCACCAGAAAACACCAAATTAGCATTTCAAGCAGCCCAAGTTTTTGATTTTGATGGTATTTGA", "aa": "MRKQFLIAYRGYSSIAPENTKLAFQAAQVFDFDGI", "aa_hexdigest": "096cf6b28b10c2b2b4fc0ba311cdc327", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3997.532699999999, "isoelectric_point": 8.188854789733888 }, "id": "JPCNHPFPBM_883", "locus": "GCA000292505_00883" }, { "type": "cds", "sequence": "contig_1", "start": 484438, "stop": 484707, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTCATTATGAACCTGATGGAGAATGAGTTTGAGCAGGATTCTTTGGTGATGATCAAAAATGAAATAAGGATGCAACTGTTAAATGAGCTAGAGAAAGAGATTATTTACCACTTATTGGTGATGAAGTTTATGGTAGGTACAACAATAAAGGTGAGTGGATTTGATATGGTTTCTATGATGAATCTGGAGACTGAGTTCTTGTTGATGAACAATGAAAGAATCGTCAACCACGAATTAACGAGGCACCTAAGTTTTGAGAGAAATTAA", "aa": "MVIMNLMENEFEQDSLVMIKNEIRMQLLNELEKEIIYHLLVMKFMVGTTIKVSGFDMVSMMNLETEFLLMNNERIVNHELTRHLSFERN", "aa_hexdigest": "8707d5869708f37541a4b5a16d0e55d5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10682.52679999999, "isoelectric_point": 4.9306348800659165 }, "id": "JPCNHPFPBM_884", "locus": "GCA000292505_00884" }, { "type": "cds", "sequence": "contig_1", "start": 484733, "stop": 487138, "strand": "-", "frame": 3, "gene": null, "product": "Protein P200", "db_xrefs": [ "GO:0020035", "SO:0001217", "UniRef:UniRef50_Q49429", "UniRef:UniRef90_Q49429" ], "nt": "TTGGAATATAAAGTAGATTTCCTTGAAACTGAACCAAAATCATTATTTGATGAAAAAACAACAATAGTTGTAGAAAGTGAACCACCTTTCATTCAACCTGACCTATCACTTGAATTAGATTCTGTAAATGATGTTGATAAATCATTAGAAACTAAAACTACATCTGTTGAATTAAATCATGAAGAAATTGGTAATGAATTTATTAATTTAGATGTTTCTGAAAAAGAAGTTCAAGAACAACCAACAACACAACTAGAAACTGATAGTGAGTTCGTATTACCAACATATCAAATAGTTGAAGACAGTTTCACTGAAAGTGCTGAAACTCCTAATGAATTTTCATCTGAACAAAAAGATACGCTTGAATTCATTAGTCAAACTCAAGAGGTTGAGACTTCTGAATCAAATGTACCCACAGTTGAACAAGAAACAAAATTATTTGAACATCAAGATGAAAATAACTTATTCACTCCTCTACCGCTTGATTTAACTGAAATCATTGAATCAAATGCTTTATTTGATTCAAAACCAGACGAAAAAGAGAGTTCTGAATCAGAATTCCAACCTACTTTCAAAGAAATTAAATTAGATTCAACTGTTGAAGTTCCTCAAGAATCATCACAAGTTGAAGCTACTTTTGATACTGTTCAACCAGAAGCAGTTTTTGATGAAATTAAAACTCAAGAACTACAGCCTGAAGCTACTACTGAAGTTGTTTTTGATGATCATTTTCAACCAGATGTTCAACCTGAACAAACTCCTCAAGAAGCTAAATTTGATTCACCAGTCGAAATTCCCCAAGAATCATCGCAAGCTGAATTTCATGCTGAACAAATCTCTGATGAAATTAAATTAGAAGAAAAAACTGAAGCAGTTTTTGATCATCAACAATTAGAAAATCAGTCTGAAGAAACAGTTGTTACACCTACTGAAGTTACTGCTTTTGAACCAGAAACAATTGAAACCCAATTAGAACCTAGCTCTGAAGATCAACCATCTGAACCTGCATTAGATCAAAATCATCCAGAAATTGTTACTGCTGAAGTTGAACAAATCTTTGATGGAACTAAGTTAGAAGATTTAAAACTAGAGGAAGCTAGCTTTGATAATGTTGAAAATAATGAAGTTCAACCTAAAGAAACAGAAGCTGAAATTACTTTTGATGAAACTAAAGAACTTCAACAAGAAACCTCCTCTGAACCATTATCAACAGAAGAATTAAAATCTGAAGCTACTTTTGATAATGTATCTGAAGCTGCATCAGAATCAGTTTTTGAAAAACCTCAATTAGAAACACAGACTGAAAAAATATTAGAAGAGGAACCTAAATCTGAACCAGTTGATCAATTAACAACTGAAGCTAGCTTTGATACTGTTAAACATGAAGCAGTTTTTGATAAAAATCAAACTCAAACTGAGGGATTGGAAGAACCTCAAGTTTCTAGTGAAGCTGAAGTAGTTGATCAAACAACTACTGATACTGTTGGAGAACCTGAAGCAGTTTTTGATGTTCAACCTGAAAAAACTACTGAAGTTAAGTTTGATGATGTTGAAAATCAACAAAAAGTTATTTCTGAACCTCAAGTAGAACAACAACCTGGAGAAGCAGTTTTTGAACCATCTGCAGAAGCTAAATTTGATTCACCAGTTGAATCGGTTCAAGATTCACAACCTGAACCTGTCCTTGAAGAAGTTCAAACTCAACCAGAAATTCAACCTGTAGAATCTCAACCAGAAGCTACCTTTGATACTGTTCAACCTGAACAAACTCCTCAAGAAGCTAAATTTGATTCACCAGTTGAAACTGTTGAACAACCTGAATTTAGTAGTGAACCAACTCAACAACATGTTGAATCAGAAGCTAGTTTTGATGAACCTAATTATGACTTTGATGAACCTAACTATGATTTTGATCAACCTAGTTATGATTCAGATCTACAACCATCAGAACCTCAATATGATGTAGATGAACCTAATTATGACTTTGATGAACCAAATTATGAAATTGAATCAAAACCATCAGAGCCACAATTTGAACCTCAAGTAGAACAACAACCTGGAGAAGCAGTTTTTGAACCATCTGCAGAAGCTAAATTTGATTCACCAGTTGAATCGGTTCAAGATTCACAACCTGAACCTCTCCTTGAAGAAGTTCAAACTCAACCAGAAATTCAACCTGTAGAATCTCAACCAGAAGCTACCTTTGATACTGTTCAACCTGAACAAACTCCTCAAGAAGCTAAATTTGATTCACCAGTTGAAACTATTCAAGAACCTCAAGTTTCTAGTGAACCTGAAGTAGTTGTTCAACCTAACTTTGAAGAACGAAAACCTGAAACAGTATTGGAAGAACCTCAAGCTGATGAAATTCAACCAGAAGCTAGTGAAGAAGAATCACTTGATTGA", "aa": "MEYKVDFLETEPKSLFDEKTTIVVESEPPFIQPDLSLELDSVNDVDKSLETKTTSVELNHEEIGNEFINLDVSEKEVQEQPTTQLETDSEFVLPTYQIVEDSFTESAETPNEFSSEQKDTLEFISQTQEVETSESNVPTVEQETKLFEHQDENNLFTPLPLDLTEIIESNALFDSKPDEKESSESEFQPTFKEIKLDSTVEVPQESSQVEATFDTVQPEAVFDEIKTQELQPEATTEVVFDDHFQPDVQPEQTPQEAKFDSPVEIPQESSQAEFHAEQISDEIKLEEKTEAVFDHQQLENQSEETVVTPTEVTAFEPETIETQLEPSSEDQPSEPALDQNHPEIVTAEVEQIFDGTKLEDLKLEEASFDNVENNEVQPKETEAEITFDETKELQQETSSEPLSTEELKSEATFDNVSEAASESVFEKPQLETQTEKILEEEPKSEPVDQLTTEASFDTVKHEAVFDKNQTQTEGLEEPQVSSEAEVVDQTTTDTVGEPEAVFDVQPEKTTEVKFDDVENQQKVISEPQVEQQPGEAVFEPSAEAKFDSPVESVQDSQPEPVLEEVQTQPEIQPVESQPEATFDTVQPEQTPQEAKFDSPVETVEQPEFSSEPTQQHVESEASFDEPNYDFDEPNYDFDQPSYDSDLQPSEPQYDVDEPNYDFDEPNYEIESKPSEPQFEPQVEQQPGEAVFEPSAEAKFDSPVESVQDSQPEPLLEEVQTQPEIQPVESQPEATFDTVQPEQTPQEAKFDSPVETIQEPQVSSEPEVVVQPNFEERKPETVLEEPQADEIQPEASEEESLD", "aa_hexdigest": "40bf9fa31844b79e2f6ac758ec994188", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 484430, "stop": 487438, "observations": { "mutation": [ 484450, 484492, 484513, 484621, 484645, 484675, 484681, 484735 ], "stop": [ 484450, 484492, 484513, 484621, 484645, 484675, 484681, 484735 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49429", "query_cov": 1.0, "subject_cov": 0.49566831683168316, "identity": 0.986, "score": 1037.0, "evalue": 0.0, "gene_start": 1, "gene_end": 801, "reference_start": 636, "reference_end": 1436, "reference_sequence": "MPKTTKNKNKNTTPKSKTKKYLESANKKSVTKPKKEQDKVENLFDQPFLGEIKKNILKKTKSFNSKKKETVKSKSKSPIDFFDETKRGVFIVPPETDILSRRELNQKTVVNTVPNQTSSYPTINENKLVELNNQPETKVLETKKDSFTTTIREKKLNPEDSQAFWYIFVGDRKYGFWKNHTWVWLGYFDQLQRWNYFKVIETVEVPQEHAAFIKQRPADIDFWRPLVGNPNYGFVQNNTWIWKGFFDKKLNWIPDPVRFTEEALGHTDSLVDEIEKKTISEQPYWEQENDIVVTVFNTKSLASSLENELLLENSSEEQPVIEEVKPRRNEVIFRNPVTKLHFEKEKFEFLNPVKETNETIPLIEIVKEEVKVESEVEAPVEIEPEAACEPETTIPEVETVFVYEDDLKGLDSNQTQAGNVPEVETVFVYEDDLKGLDSIIKDDQQHDEIAKHVEHLSQDYSKEIKDSAKADLSNISDDIDSVWKEFGSFTDETQKSVEEKSQVDEIILDANNDFINESLFRDEVVNNIDSQINETVSEQQFEPTYSVNEFQQEFSEPVVSDEKIKETNSDESVNTDLTALFSEKLVNEVLLTNEYVDVNAPFSTETEVKVSSELPKSELVDEITFINNDPKPQEGLEYKVDFLETEPKSLFDEKTTIVVESEPPFIQPDLSLELDSVNDVDKSLETKTTSVELNHEEIGNEFINLDVSEKEVQEQPTTQLETDSEFVLPTYQIVEDSFTESAETPNEFSSEQKDTLEFISQTQEVETSESNVPTVEQETKLFEHQDENNLFTPLPLDLTEIIESNALFDSKPDEKESSDSELQPTFKEIKLDSTVEVPQESSQVEATFDTVQPEAVFDEIKTQELQPEATTEVVFDDHFQPDVQPEQTPQEAKFDSPVEIPQESSQAEFHAEQISDEIKLEEKTEAVFDHQQLENQSEETVVTPTEVTAFEPETIETQLEPSSEDQPSEPALDQNHPEIVTAEVEQIFDGTKLEDLKLEEANFDNVENNEVQPKETEAEITFDETKELQQETSSEPLSTEELKSEATFDNVSEAESEAVFEKPQLETQTEKILEEEPKSEPVDQLITEASFDTVKHEAVFDKNQTQTEGLEEPQVSSEAEVVDQTTTDTVGEPEAVFDVQPEKTTEVKFDDVENQQKVISEPQVEQQPGEAVFEPSAEAKFDSPVESVQDSQPEPVLEEVQTQPEIQPVESQPEATFDTVQPEQTPQEAKFDSPVETVEQPEFSSEPTQQHVESEASFDEPNYDFDEPNYDFDQPSYDSDLQPSEPQYDVDEPNYDFDEPNYEIESKPSEPQFEPQVEQQPGEAVFEPSAEAKFDSPVESVQDSQPEPLLEEVQTQPEIQPVESQPEATFDTVQPEQTPQEAKFDSPVETIQEPQVSSEPEVVVQPNFEERKPETVLEEPQADEIQPEASEEESLDWELLVGNNSYGHYEPDGEWVWAGFFGDDQKWNKDATVKWARERDYLPLIGDEVYGRYNNKGEWIWYGFYDESGDWVLVDEQWKNRQPRINEAPKFWEKLIGNEEYGYYEDNEWNWYDGEFDSEGNWLVFQSEETENLNEDITKDIPALEGYDIDSIDADEWLSQFSADDAKDVFGSNDKK" }, "paralog": false, "identity": 0.9790419161676647, "score": 1442.0, "evalue": 0.0, "description": "Internal stop codon at 484450, 484492, 484513, 484621, 484645, 484675, 484681, 484735. Nonsense mutation around 484450, 484492, 484513, 484621, 484645, 484675, 484681, 484735.", "psc": { "uniref90_id": "UniRef90_Q49429", "product": "Protein P200", "uniref50_id": "UniRef50_Q49429", "go_ids": [ "GO:0020035" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49429", "UniRef:UniRef50_Q49429", "GO:0020035" ] }, "pscc": { "uniref50_id": "UniRef50_Q49429", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49429" ], "product": "Protein P200" } }, "id": "JPCNHPFPBM_885", "locus": "GCA000292505_00885" }, { "type": "cds", "sequence": "contig_1", "start": 487197, "stop": 487394, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGAAGTTCACTTGAAACCTTAACTTCTGTTTCTGTTGAAAAAGGAGCATTAACATCAACATATTCATTTGTTAAGAGTACCTCATTAACAAGTTTTTCACTAAATAAAGCAGTTAAATCAGTATTAACACTTTCATCACTGTTTGTTTCTTTTATCTTTTCATCTGAAACAACAGGTTCGCTAAATTCTTGTTGA", "aa": "MGSSLETLTSVSVEKGALTSTYSFVKSTSLTSFSLNKAVKSVLTLSSLFVSFIFSSETTGSLNSC", "aa_hexdigest": "0e8fc2df2ac5f2383f8cab50b3e41f9f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6796.6309999999985, "isoelectric_point": 7.899843025207519 }, "id": "JPCNHPFPBM_886", "locus": "GCA000292505_00886" }, { "type": "cds", "sequence": "contig_1", "start": 487500, "stop": 487667, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATCATTATTAGCATCTAAAATAATTTCATCTACTTGTGATTTTTCTTCTACACTTTTTTGTGTTTCATCAGTAAAAGAACCGAATTCTTTTCAAACTGAATCAATATCATCAGAAATATTAGATAAATCTGCTTTAGCACTATCTTTTATTTCTTTAGAATAA", "aa": "MKSLLASKIISSTCDFSSTLFCVSSVKEPNSFQTESISSEILDKSALALSFISLE", "aa_hexdigest": "092228fd981c62e73b5e6ecaad045e11", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5921.717399999998, "isoelectric_point": 4.8005872726440435 }, "id": "JPCNHPFPBM_887", "locus": "GCA000292505_00887" }, { "type": "cds", "sequence": "contig_1", "start": 487965, "stop": 488249, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTCATTAGTTTCTTTAACTGGATTTAAGAATTCAAATTTTTCTTTTTCAAAATGTAATTTAGTTACTGGATTACGGAAGATAACTTCATTTCTTCTTGGTTTAACTTCTTCAATTACCGGCTGCTCTTCACTTGAATTTTCAAGTAATAGTTCGTTTTCTAGAGAAGAAGCTAAGCTCTTGGTATTAAATACTGTAACAACAATGTCATTTTCCTGTTCTCAGTATGGTTGTTCAGAAATAGTCTTTTTCTCTATTTCATCAACTAAGCTATCAGTATGA", "aa": "MVSLVSLTGFKNSNFSFSKCNLVTGLRKITSFLLGLTSSITGCSSLEFSSNSSFSREEAKLLVLNTVTTMSFSCSQYGCSEIVFFSISSTKLSV", "aa_hexdigest": "4efbb505a0c6d021d68f16798ce2e917", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10122.533399999993, "isoelectric_point": 8.836504173278808 }, "id": "JPCNHPFPBM_888", "locus": "GCA000292505_00888" }, { "type": "cds", "sequence": "contig_1", "start": 488549, "stop": 489043, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAAAACAACAAAGAATAAAAACAAAAACACCACCCCTAAATCAAAAACCAAAAAGTATTTAGAATCAGCTAACAAGAAGAGTGTTACTAAACCAAAAAAAGAACAAGATAAAGTTGAAAATCTTTTTGATCAACCTTTTTTAGGTGAAATCAAAAAAAACATTCTCAAGAAAACAAAGTCTTTTAATAGTAAGAAAAAGGAAACAGTTAAATCAAAGAGCAAATCACCTATAGATTTTTTTGATGAAACCAAGCGCGGTGTTTTTATTGTTCCACCTGAAACAGATATCTTAAGTAGAAGAGAACTTAATCAAAAAACTGTTGTTAATACAGTTCCAAATCAAACAAGCAGTTATCCAACCATTAATGAAAATAAGTTGGTTGAATTAAACAATCAACCAGAAACCAAAGTTCTAGAAACAAAAAAAGATAGTTTTACAACAACTATTAGAGAAAAAAAATTAAATCCAGAAGATTCACAAGCGTTCTGA", "aa": "MPKTTKNKNKNTTPKSKTKKYLESANKKSVTKPKKEQDKVENLFDQPFLGEIKKNILKKTKSFNSKKKETVKSKSKSPIDFFDETKRGVFIVPPETDILSRRELNQKTVVNTVPNQTSSYPTINENKLVELNNQPETKVLETKKDSFTTTIREKKLNPEDSQAF", "aa_hexdigest": "4710bbf8d9536695c6d5ba638c063d09", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18893.3926, "isoelectric_point": 9.87360706329346 }, "id": "JPCNHPFPBM_889", "locus": "GCA000292505_00889" }, { "type": "cds", "sequence": "contig_1", "start": 489043, "stop": 489132, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGCTAACTTTTGTTATCTTGATATTAAAAATTACAATTTAGTGAGTCAGATAACTTTAGTCACTAGTGCTAAGTATTTTTATTAA", "aa": "MYANFCYLDIKNYNLVSQITLVTSAKYFY", "aa_hexdigest": "9b8a01e0305593bdadca5e8a9361e12d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3487.005, "isoelectric_point": 7.867093086242677 }, "id": "JPCNHPFPBM_890", "locus": "GCA000292505_00890" }, { "type": "cds", "sequence": "contig_1", "start": 489328, "stop": 489531, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07650.23" ], "nt": "TTGTTAAAAATTCACCTAGTAATTAGTGTTCCAAAATTAAGCCAAAAAAAGATTATCATTGGAAAAAATGCAGAAATGATCAAAGCAATTGGGATAGCAACTCGCAAAAAACTATTAAATCATTTTGACTGTGATATTTTTATTGATATTTTTGTAAAAACAGAAAAACAAAAATTGCCTGTTTATAGTTTTCTTTCTAAATAA", "aa": "MLKIHLVISVPKLSQKKIIIGKNAEMIKAIGIATRKKLLNHFDCDIFIDIFVKTEKQKLPVYSFLSK", "aa_hexdigest": "fe21dd93fe1b44978f05834f70f587a7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07650.23", "name": "KH_2", "length": 58, "aa_cov": 0.8059701492537313, "hmm_cov": 0.9827586206896551, "evalue": 5.436945079275702e-11, "score": 37.331119537353516, "start": 4, "stop": 57 } ], "seq_stats": { "molecular_weight": 7697.348799999998, "isoelectric_point": 9.945747184753419 }, "id": "JPCNHPFPBM_891", "locus": "GCA000292505_00891" }, { "type": "cds", "sequence": "contig_1", "start": 489721, "stop": 490200, "strand": "-", "frame": 1, "gene": "era", "product": "GTPase Era", "db_xrefs": [ "COG:COG1159", "COG:J", "EC:3.6.5.-", "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0019843", "GO:0043024", "GO:0070181", "SO:0001217", "UniRef:UniRef50_P47627", "UniRef:UniRef90_P47627" ], "nt": "ATGAAAGTTTTAAAAGTTGGTGTTTTAGGACCTACTAATGCAGGAAAATCAACGCTAATTAATTTTTTACATAACGATGATAGTCTGATGGTATCATCCATGAATAACACAACGCTTCTTTCTATTTCCACTGAGGTAATTAACCAAGCTAATAAAAATATTGTTTTTATTGATGTCCCTGGTTTTACTGAGAAAAAACATTCAAATTATGAATTAATAACTAAAGAAATTCGCAAAGCATTAAGTGGAATTGATGTATTGCTTTTAGTTGTTAGAAGTGATCAGAATAACAAGATAGAATTTTTAAAAACTCAATTACAACAATTAAAGCGCTATCAAAATTTAACAAGAATTTTTTTAATTAATAAATTTCATCAAAAAAGTTTAAGTGAAGTTAATAAAGCAATTATTTTAGAAGAGTTTAAACCACAAAAAACAATTGAAATAAACTTACTTAAGTTTGATAAAAATCTCTTTTGA", "aa": "MKVLKVGVLGPTNAGKSTLINFLHNDDSLMVSSMNNTTLLSISTEVINQANKNIVFIDVPGFTEKKHSNYELITKEIRKALSGIDVLLLVVRSDQNNKIEFLKTQLQQLKRYQNLTRIFLINKFHQKSLSEVNKAIILEEFKPQKTIEINLLKFDKNLF", "aa_hexdigest": "fe69406db23df748a8201ca25f056780", "start_type": "ATG", "rbs_motif": null, "genes": [ "era" ], "truncated": "5-prime", "pseudogene": { "start": 489418, "stop": 490200, "observations": { "mutation": [ 489723 ], "stop": [ 489723 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47627", "query_cov": 1.0, "subject_cov": 0.5482758620689655, "identity": 1.0, "score": 300.0, "evalue": 8.66e-101, "gene_start": 1, "gene_end": 159, "reference_start": 1, "reference_end": 159, "reference_sequence": "MKVLKVGVLGPTNAGKSTLINFLHNDDSLMVSSMNNTTLLSISTEVINQANKNIVFIDVPGFTEKKHSNYELITKEIRKALSGIDVLLLVVRSDQNNKIEFLKTQLQQLKRYQNLTRIFLINKFHQKSLSEVNKAIILEEFKPQKTIEINLLKFDKNLFWSIFKQVELRYNIFRKDINFIDANNDDFKILEGLREQIIFYCKNEIPHIARIEIIEKSFNKEKNLLKIHLVISVPKLSQKKIIIGKNAEMIKAIGIATRKKLLNHFDCDIFIDIFVKTEKQKLPVYSFLSK" }, "paralog": false, "identity": 0.9961538461538462, "score": 498.0, "evalue": 1.23e-181, "description": "Internal stop codon at 489723. Nonsense mutation around 489723.", "psc": { "uniref90_id": "UniRef90_P47627", "gene": "era", "product": "GTPase Era", "ec_ids": [ "3.6.5.-" ], "uniref50_id": "UniRef50_P47627", "cog_id": "COG1159", "cog_category": "J", "go_ids": [ "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0019843", "GO:0043024", "GO:0070181" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47627", "EC:3.6.5.-", "UniRef:UniRef50_P47627", "COG:COG1159", "COG:J", "GO:0000028", "GO:0003723", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0005886", "GO:0019843", "GO:0043024", "GO:0070181" ] }, "pscc": { "uniref50_id": "UniRef50_P47627", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47627" ], "product": "GTPase Era" } }, "id": "JPCNHPFPBM_892", "locus": "GCA000292505_00892" }, { "type": "cds", "sequence": "contig_1", "start": 490329, "stop": 490652, "strand": "-", "frame": 2, "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "db_xrefs": [ "COG:COG0319", "COG:J", "EC:3.1.-.-", "GO:0004222", "GO:0004521", "GO:0004540", "GO:0005737", "GO:0006364", "GO:0008270", "GO:0042254", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47628", "UniRef:UniRef90_P47628" ], "nt": "TTGAAGTCATCTTTTAGCATTAATTCAAGCCGCCTTTTTAAGCGCTTTTTTGGTAAAAATTACCAAGCAGGAGTCGAACTTTGTTTCCAAATTATTAAAAGCAGTTTAAACCTATCGTTTGACCCAGAATTTGCGCTTTTAATTGTTTCCTGTTCAAAAATGAAAAAACTGAACAAACAGTTTTTAAAACGTAGAGGTTGTACTGATGTAATTAGTTTGTGCTATAACGAAACTGAAACCGGTTTTTCACTAGCAATCGGCGAAATTTTTTTGTGTCCTAAATACATCTTTAAAAAAGCTAAAGAGATTGGTTGTAACAATTGA", "aa": "MKSSFSINSSRLFKRFFGKNYQAGVELCFQIIKSSLNLSFDPEFALLIVSCSKMKKLNKQFLKRRGCTDVISLCYNETETGFSLAIGEIFLCPKYIFKKAKEIGCNN", "aa_hexdigest": "09db2ba6b472e48feac7092f8ce790c2", "start_type": "TTG", "rbs_motif": null, "genes": [ "ybeY" ], "truncated": "5-prime", "pseudogene": { "start": 490188, "stop": 490652, "observations": { "mutation": [ 490199, 490331 ], "stop": [ 490199, 490331 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47628", "query_cov": 1.0, "subject_cov": 0.6948051948051948, "identity": 1.0, "score": 218.0, "evalue": 1.45e-71, "gene_start": 1, "gene_end": 107, "reference_start": 1, "reference_end": 107, "reference_sequence": "MKSSFSINSSRLFKRFFGKNYQAGVELCFQIIKSSLNLSFDPEFALLIVSCSKMKKLNKQFLKRRGCTDVISLCYNETETGFSLAIGEIFLCPKYIFKKAKEIGCNNWFLLTRCLIHGILHLFEFNHEESEAFESVTMFFQDAIHEEILSVWKF" }, "paralog": false, "identity": 0.9805194805194806, "score": 304.0, "evalue": 6.48e-108, "description": "Internal stop codon at 490199, 490331. Nonsense mutation around 490199, 490331.", "psc": { "uniref90_id": "UniRef90_P47628", "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "ec_ids": [ "3.1.-.-" ], "uniref50_id": "UniRef50_P47628", "cog_id": "COG0319", "cog_category": "J", "go_ids": [ "GO:0004222", "GO:0004521", "GO:0004540", "GO:0005737", "GO:0006364", "GO:0008270", "GO:0042254", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47628", "EC:3.1.-.-", "UniRef:UniRef50_P47628", "COG:COG0319", "COG:J", "GO:0004222", "GO:0004521", "GO:0004540", "GO:0005737", "GO:0006364", "GO:0008270", "GO:0042254", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47628", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47628" ], "product": "Endoribonuclease YbeY" } }, "id": "JPCNHPFPBM_893", "locus": "GCA000292505_00893" }, { "type": "cds", "sequence": "contig_1", "start": 490690, "stop": 490884, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MG389", "db_xrefs": [ "GO:0016020", "SO:0001217", "UniRef:UniRef50_P47629", "UniRef:UniRef90_P47629" ], "nt": "ATGTTTAAAAATGAAAAAGATGAAAATCTTTTTAACAACGTTTCATGGACTAATTTTCAAGCAGAAACAACAGAAAAATCAGCAAAAAAAGCTTTTAGACTTTACAAAAAGAAAATTTCTACATCAGAAATTTCATCAGAATTGAACAAAAATCTAGTAAAGGTAGATTTAAGTGTTACTCTAGAACAGGGCTGA", "aa": "MFKNEKDENLFNNVSWTNFQAETTEKSAKKAFRLYKKKISTSEISSELNKNLVKVDLSVTLEQG", "aa_hexdigest": "3811cb96904f9a648d7c9e2d2717cc78", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 490636, "stop": 491019, "observations": { "mutation": [ 490692, 490911 ], "stop": [ 490692, 490911 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47629", "query_cov": 1.0, "subject_cov": 0.5039370078740157, "identity": 1.0, "score": 119.0, "evalue": 1.46e-33, "gene_start": 1, "gene_end": 64, "reference_start": 46, "reference_end": 109, "reference_sequence": "MKQKILGITIAFIILLLTTVAILFSVKVKQYLNVNLWTTQQQNFLMFKNEKDENLFNNVSWTNFQAETTEKSAKKAFRLYKKKISTSEISSELNKNLVKVDLSVTLEQGWYNITIMLPSKDLFEVIF" }, "paralog": false, "identity": 0.984251968503937, "score": 238.0, "evalue": 1.07e-82, "description": "Internal stop codon at 490692, 490911. Nonsense mutation around 490692, 490911.", "psc": { "uniref90_id": "UniRef90_P47629", "product": "Uncharacterized protein MG389", "uniref50_id": "UniRef50_P47629", "go_ids": [ "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47629", "UniRef:UniRef50_P47629", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P47629", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47629" ], "product": "Uncharacterized protein MG389" } }, "id": "JPCNHPFPBM_894", "locus": "GCA000292505_00894" }, { "type": "cds", "sequence": "contig_1", "start": 490909, "stop": 491019, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGCAGAAAATTTTAGGAATAACAATTGCTTTTATTATTTTGCTTTTAACAACAGTTGCTATCTTGTTTTCAGTAAAGGTAAAACAATATCTTAATGTAAATCTTTGA", "aa": "MKQKILGITIAFIILLLTTVAILFSVKVKQYLNVNL", "aa_hexdigest": "46036968c72f159cecceacf64166a32e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4061.0538000000006, "isoelectric_point": 10.176350593566895 }, "id": "JPCNHPFPBM_895", "locus": "GCA000292505_00895" }, { "type": "cds", "sequence": "contig_1", "start": 491023, "stop": 491370, "strand": "-", "frame": 1, "gene": null, "product": "ABC-type enterochelin transport system, ATPase component", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAV5N885" ], "nt": "ATGCAAACAGAAGCAAATGATTTTGGCTTTAGTGAGGCTTTATTTAATAATCAGAATCCACATATCTATCAGTTACTTTTTGATGCAGGTGTTCAAAACCAAACAAAGTTAAGTTCAGGACAAAAACAAATTTTGCAACTATTCTTACTTAGCAACATTAAGAATAAGGTGATCTTACTTGATGAATGCATGAATGCAATAGCACCTGAAATTAAAAACCGTGTTTATCAGCTATTAGTAAAACCACTTACTTTAAATAATTTTGTAGTCTTAGTTGAGCATGATCTGAGTTTTGCAAGTGAAGCTCAAAACAAGATTAACCTAACTAATTACTTAAGAAATAGTTAA", "aa": "MQTEANDFGFSEALFNNQNPHIYQLLFDAGVQNQTKLSSGQKQILQLFLLSNIKNKVILLDECMNAIAPEIKNRVYQLLVKPLTLNNFVVLVEHDLSFASEAQNKINLTNYLRNS", "aa_hexdigest": "d9e4a607d87bdad315209b09af887035", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAV5N885", "query_cov": 0.8695652173913043, "subject_cov": 0.9803921568627451, "identity": 0.67, "score": 132.0, "evalue": 3.65e-38, "valid": false, "product": "ABC-type enterochelin transport system, ATPase component", "uniref50_id": "UniRef50_A0AAV5N885" }, "pscc": { "uniref50_id": "UniRef50_A0AAV5N885", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAV5N885" ], "product": "ABC-type enterochelin transport system, ATPase component" }, "genes": [], "id": "JPCNHPFPBM_896", "locus": "GCA000292505_00896" }, { "type": "cds", "sequence": "contig_1", "start": 491632, "stop": 492312, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAGCAAAAAAGCTTTGTTTACTTTAGTAATAAATCACCAAATCAATTAATAGAGCGACAATTTTATTTAAAAGAAGTTCTTTCCTTTTTTAACTTTTATATTCCCAATTTAATTATTAGTTGTGTTGTTGCACTGATTATCGGTGTTTTAATAGGAATTAATCAATTAGAATTTTTATTGATTGCCATTGCACAAATAGTAGTCAATGCTGGCTTATTTTGTTATGACTTCTTTTTTACTAAAAAAATTACAAAGAAGGAAATTCCTTATGTTGAATTGCAAAACAAAATTAGTTTACAGCTAGATGAAAATTTGCGTGAAGAACAAAATAAAAAGCGCTTTAATTTCTTAATGTTGAATTTTCGTAAAGCACTTTTACAAAATCAAAACATTAACAACCAGAAGGAAATTAATCGTCTAACTATAGAAAACATTAAAAGCTTCTTCCAACAAGGTTTTGATTTTGCTATATTAGGTTTGGGGGTGATTGGTATTATTGAACAGCGTTATCAATTGAGCTTTTTATTCTATGTTTTTGGTATCCAATCACTTTTTTCAACTTATGCTACAAGAATTGTTCAATTTGGTGCTGCAATAAACATTTATCACTATTGTCGTGAGAAATTAGTTAATTTGTTTATAGAAACCAAAAAGGATGAGGGTATTAAAGTAAATTGA", "aa": "MQQKSFVYFSNKSPNQLIERQFYLKEVLSFFNFYIPNLIISCVVALIIGVLIGINQLEFLLIAIAQIVVNAGLFCYDFFFTKKITKKEIPYVELQNKISLQLDENLREEQNKKRFNFLMLNFRKALLQNQNINNQKEINRLTIENIKSFFQQGFDFAILGLGVIGIIEQRYQLSFLFYVFGIQSLFSTYATRIVQFGAAINIYHYCREKLVNLFIETKKDEGIKVN", "aa_hexdigest": "2ba9b9a482bff619f5503df6163b3eb5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26553.785900000013, "isoelectric_point": 9.24955310821533 }, "id": "JPCNHPFPBM_897", "locus": "GCA000292505_00897" }, { "type": "cds", "sequence": "contig_1", "start": 492712, "stop": 493005, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATCATTTATCAAGAACAACAGAATGAGTGTGGAATTTGCGTAATTGGTATGCTTGCCAATGCAATCCATGATGAAAAGTATGTTCATGATGAATTACTAGAACAAATTAACTTACCACCAAATGGCTTAAGCTTATTTGAAATGGAAAGTTATGGCAAAAAGTTTGGTCTTGAAATCAACAGTTATCAATTAACATTTCAAGAACTAAAAGAACTTGATAGCAAATTCATAATAGTTCATTTTAAAGATCACTTTGTCATAGTTAAAAACAAACATGAAAATAGTTGA", "aa": "MKIIYQEQQNECGICVIGMLANAIHDEKYVHDELLEQINLPPNGLSLFEMESYGKKFGLEINSYQLTFQELKELDSKFIIVHFKDHFVIVKNKHENS", "aa_hexdigest": "2f88fd873904c40c1cdda9a37a3c97f8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11357.908999999987, "isoelectric_point": 5.241998863220213 }, "id": "JPCNHPFPBM_898", "locus": "GCA000292505_00898" }, { "type": "cds", "sequence": "contig_1", "start": 493113, "stop": 493346, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00883.26" ], "nt": "ATGCACCCTGATTATTTAAAACCTTTACAGCTAACAAAACTTGCTGATTTGCAAAATTCTACTAGTGCAAGAGGTGCTGGATCTTCAAGAGCAGCTTGTTTCCTTGCAGAATTTAGAGAAGGTGTATCTTTGATCCATTGTGATATTGCATCAACTGCTTCCATTGAGAACCTTGGACAAGGTGTTTTAGTGAGAACCTTGTACGAACGTGCTAGTCAGCTTGCAAATAAATAA", "aa": "MHPDYLKPLQLTKLADLQNSTSARGAGSSRAACFLAEFREGVSLIHCDIASTASIENLGQGVLVRTLYERASQLANK", "aa_hexdigest": "35eaeb6e96a5625f289f9cfdcb7639f7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00883.26", "name": "Peptidase_M17", "length": 74, "aa_cov": 0.8571428571428571, "hmm_cov": 0.972972972972973, "evalue": 1.421909399183151e-10, "score": 35.99742126464844, "start": 2, "stop": 67 } ], "seq_stats": { "molecular_weight": 8305.357199999993, "isoelectric_point": 7.827573966979982 }, "id": "JPCNHPFPBM_899", "locus": "GCA000292505_00899" }, { "type": "cds", "sequence": "contig_1", "start": 493401, "stop": 494456, "strand": "-", "frame": 2, "gene": "pepA", "product": "leucyl aminopeptidase", "db_xrefs": [ "COG:COG0260", "COG:E", "EC:3.4.11.1", "GO:0005737", "GO:0006508", "GO:0008233", "GO:0030145", "GO:0070006", "SO:0001217", "UniRef:UniRef50_P47631", "UniRef:UniRef90_P47631" ], "nt": "ATGAGAATAAATAAACCTTTTAGTGATGATAGCAACACAGTTGTTTTTGTGAGTTCAAAAACATATGGTGTAAAAGAAGAAGCTGCACATAATCCTAATGTTGAATTTGGTGTTGTTTTACCAACTGATTTTCCTGCTTTCAACCGTGCTTTAGTTCAATTTCTTAAAAGAAAGAAAACCAAATTAAACATTAATCTTGACAGTCTTATAGAACTTTATAAGAAAAATGAAAATAGTGGTTGTTTTCATACTGCGATAAAAACTGTTATTACAAGTGTTACTTTTTGTGAAACTACTCCTTTCACAATGAAAACTAAACCTGAAAAAAATGTTGAAGTTGCTGTTCAATGTGCTGTTGAATATCACAACTTAGTTAAAGAATATGAAACAGTAGGCGAATATGTTAACCTAGCAAGAGAATTACAAGACACTCCTTCAGATCTACTTTATCCAGAAGTATTTGTTAAACATTTTGAAAAGGCTGCAAGTAAATTGCCTGTAAAAATAAAAGTTCTTAAACAATCAGATCTAATTAAGAAAAAAATGGGTTTACTTTTAGGGGTTAATCAAGGCTCTGAAAGAGAAGCACGTTTACTTGTTATTAGTTATCAAGCTAATAAAAATTCCAAAGAAAAACTTGCTTTTGTAGGGAAAGGAATTACTTATGATTCAGGCGGAATGAACATTAAAACAGGTGATTATATGCGTGGCATGAAATATGACATGAGTGGTGCGGCTATAGTATGTTCTACTGTTTTGGCATTAGCTAAAAACAAGGTTAAAACCAATGTTGTTGCAGTAGCAGCTCTTACTGAAAATCTTCCTGGTGCTAAGGCGCAACGTCCTGATGACATTAAGATAGCATACAATGGTAAAAGTGTTGAAATAGATAACACCGATGCTGAAGGAAGATTGGTTTTAGCTGATGCTATTACATATGCTGCTAAGGATTTAGCTGCTACACATATTATTGATGTAGCAACCCTTACTGGTTTAATGTCATACATATTGAGTACTACCTATACAGGTATTTTCAGTACTTGTGATCACCAGTGA", "aa": "MRINKPFSDDSNTVVFVSSKTYGVKEEAAHNPNVEFGVVLPTDFPAFNRALVQFLKRKKTKLNINLDSLIELYKKNENSGCFHTAIKTVITSVTFCETTPFTMKTKPEKNVEVAVQCAVEYHNLVKEYETVGEYVNLARELQDTPSDLLYPEVFVKHFEKAASKLPVKIKVLKQSDLIKKKMGLLLGVNQGSEREARLLVISYQANKNSKEKLAFVGKGITYDSGGMNIKTGDYMRGMKYDMSGAAIVCSTVLALAKNKVKTNVVAVAALTENLPGAKAQRPDDIKIAYNGKSVEIDNTDAEGRLVLADAITYAAKDLAATHIIDVATLTGLMSYILSTTYTGIFSTCDHQ", "aa_hexdigest": "1fe2edb75aa2c4cca60684d99bdb9b00", "start_type": "ATG", "rbs_motif": null, "genes": [ "pepA" ], "truncated": "5-prime", "pseudogene": { "start": 493113, "stop": 494456, "observations": { "mutation": [ 493358, 493403 ], "stop": [ 493358, 493403 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47631", "query_cov": 1.0, "subject_cov": 0.785234899328859, "identity": 0.997, "score": 671.0, "evalue": 1.4e-241, "gene_start": 1, "gene_end": 351, "reference_start": 1, "reference_end": 351, "reference_sequence": "MRINKPFSDDSNTVVFVSSKTYGVKEEAAHNPNVEFGVVLPTDFPAFNRALVQFLKRKKTKLNINLDSLIELYKKNENSGCFHTAIKTVITSVTFCETTPFTMKTKPEKNVEVAVQCAVEYHNLVKEYETVGEYVNLARELQDTPSDLLYSEVFVKHFEKAASKLPVKIKVLKQSDLIKKKMGLLLGVNQGSEREARLLVISYQANKNSKEKLAFVGKGITYDSGGMNIKTGDYMRGMKYDMSGAAIVCSTVLALAKNKVKTNVVAVAALTENLPGAKAQRPDDIKIAYNGKSVEIDNTDAEGRLVLADAITYAAKDLAATHIIDVATLTGLMSYILSTTYTGIFSTCDHQWESFKKAACSAGEPVWRLPMHPDYLKPLQLTKLADLQNSTSARGAGSSRAACFLAEFREGVSLIHCDIASTASIENLGQGVLVRTLYERASQLANK" }, "paralog": false, "identity": 0.9932885906040269, "score": 866.0, "evalue": 3.94e-321, "description": "Internal stop codon at 493358, 493403. Nonsense mutation around 493358, 493403.", "psc": { "uniref90_id": "UniRef90_P47631", "gene": "pepA", "product": "leucyl aminopeptidase", "ec_ids": [ "3.4.11.1" ], "uniref50_id": "UniRef50_P47631", "cog_id": "COG0260", "cog_category": "E", "go_ids": [ "GO:0005737", "GO:0030145", "GO:0070006", "GO:0008233", "GO:0006508" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47631", "EC:3.4.11.1", "UniRef:UniRef50_P47631", "COG:COG0260", "COG:E", "GO:0005737", "GO:0030145", "GO:0070006", "GO:0008233", "GO:0006508" ] }, "pscc": { "uniref50_id": "UniRef50_P47631", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47631" ], "product": "Probable cytosol aminopeptidase" } }, "id": "JPCNHPFPBM_900", "locus": "GCA000292505_00900" }, { "type": "cds", "sequence": "contig_1", "start": 494473, "stop": 496104, "strand": "-", "frame": 1, "gene": "groL", "product": "chaperonin GroEL", "db_xrefs": [ "COG:COG0459", "COG:O", "EC:5.6.1.7", "GO:0005524", "GO:0009408", "GO:0016853", "GO:0042026", "GO:0051082", "GO:0051085", "GO:0140662", "GO:1990220", "RefSeq:WP_009885631.1", "SO:0001217", "UniParc:UPI0000557C61", "UniRef:UniRef100_P47632", "UniRef:UniRef50_P47632", "UniRef:UniRef90_P47632" ], "nt": "ATGGCAAAGGAATTAATCTTTGGTAAAGATGCGAGAACCCGCTTGTTGCAGGGTATTAATAAGATAGCAAATGCTGTTAAAGTAACAGTAGGTCCTAAAGGCCAAAATGTTATTTTAGAGAGAAAATTTGCAAACCCATTAATTACTAACGATGGGGTTACAATCGCAAAAGAAATAGAACTTAGTGATCCAGTTGAAAATATTGGTGCTAAGGTTATTTCAGTTGCTGCAGTGTCAACTAATGACATTGCTGGGGATGGTACAACAACAGCTACCATATTAGCACAAGAAATGACAAACCGTGGTATTGAAATTATCAATAAAGGTGCTAATCCTGTTAACATCCGCAGGGGTATTGAAGATGCAAGCTTACTTATTATTAAAGAACTTGAAAAGTACTCTAAAAAAATTAATACTAACGAAGAGATAGAACAAGTTGCAGCTATCTCTTCAGGTTCTAAAGAAATTGGTAAACTGATCGCTCAAGCAATGGCTTTAGTTGGTAAAAATGGCGTGATAACAACTGATGATGCAAAAACCATTAATACAACATTAGAAACCACTGAAGGAATTGAATTTAAAGGAACATATGCATCACCTTATATGGTTAGTGATCAAGAAAAAATGGAAGTTGTTTTAGAACAACCTAAAATCTTAGTAAGCTCTTTAAAAATTAACACAATTAAAGAAATTCTTCCGCTTTTAGAAGGTAGTGTTGAAAATGGTAATCCATTATTAATTGTTGCACCTGACTTTGCAGAAGAAGTTGTTACTACTTTAGCAGTTAATAAACTCAGGGGCACCATTAATGTTGTTGCTGTTAAATGTAATGAATATGGTGAACGTCAAAAAGCAGCTTTAGAAGATTTAGCAATTAGTAGTGGAACCTTAGCATATAATAACGAAATTAATAGTGGTTTTAAAGATGTTACTGTTGATAATTTAGGTGATGCTAGAAAGGTTCAAATAGCTAAAGAAAAAACTACTGTTATTGGTGGTAAAGGCAATAAGGATAAAATCAAAAAGCATGTTGAACTTCTAAACGGAAGATTAAAACAAACCACTGACAAGTATGATTCTGATTTAATTAAAGAAAGAATTGCTTATTTAAGTCAAGGTGTTGCTGTTATCCGTGTTGGTGGTGCAACTGAACTCGCACAAAAAGAATTAAAACTCAGAATTGAAGACGCTTTAAATTCCACCAAAGCTGCAGTTGAAGAAGGGATTATCGCTGGAGGTGGTGTTGGTTTATTAAATGCTTCTTGTGTTTTAACTAACAGTAAACTAAAAGAACGATATGAAAATGAAACTAGTGTTGAAAACATTAAAGAAATCCTACTTGGTTTTGAAATTGTGCAAAAGTCTCTAGAAGCACCAGCGCGTCAAATTATTCAAAACTCAGGAGTTGACCCAGTTAAAATTCTCAGTGAATTGAAAAATGAAAAAACTGGTGTTGGCTTTGATGCTGAGACTAAAAAGAAGGTTGATATGATTGCAAATGGAATCATTGATCCCACCAAAGTAACTAAAACTGCACTTGAAAAAGCTGCTTCTGTAGCTAGTTCATTAATTACTACTAATGTTGCTGTGTATGATGTTAAAGAGAGAAAAGATAACTCCTTTTCAGAATAA", "aa": "MAKELIFGKDARTRLLQGINKIANAVKVTVGPKGQNVILERKFANPLITNDGVTIAKEIELSDPVENIGAKVISVAAVSTNDIAGDGTTTATILAQEMTNRGIEIINKGANPVNIRRGIEDASLLIIKELEKYSKKINTNEEIEQVAAISSGSKEIGKLIAQAMALVGKNGVITTDDAKTINTTLETTEGIEFKGTYASPYMVSDQEKMEVVLEQPKILVSSLKINTIKEILPLLEGSVENGNPLLIVAPDFAEEVVTTLAVNKLRGTINVVAVKCNEYGERQKAALEDLAISSGTLAYNNEINSGFKDVTVDNLGDARKVQIAKEKTTVIGGKGNKDKIKKHVELLNGRLKQTTDKYDSDLIKERIAYLSQGVAVIRVGGATELAQKELKLRIEDALNSTKAAVEEGIIAGGGVGLLNASCVLTNSKLKERYENETSVENIKEILLGFEIVQKSLEAPARQIIQNSGVDPVKILSELKNEKTGVGFDAETKKKVDMIANGIIDPTKVTKTALEKAASVASSLITTNVAVYDVKERKDNSFSE", "aa_hexdigest": "5502f1565b9d7b37257e1363472a1713", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000557C61", "ncbi_nrp_id": "WP_009885631.1", "uniref100_id": "UniRef100_P47632", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000557C61", "RefSeq:WP_009885631.1", "UniRef:UniRef100_P47632" ] }, "ips": { "uniref100_id": "UniRef100_P47632", "uniref90_id": "UniRef90_P47632" }, "psc": { "uniref90_id": "UniRef90_P47632", "gene": "groL", "product": "chaperonin GroEL", "ec_ids": [ "5.6.1.7" ], "uniref50_id": "UniRef50_P47632", "cog_id": "COG0459", "cog_category": "O", "go_ids": [ "GO:0005524", "GO:0009408", "GO:0016853", "GO:0042026", "GO:0051082", "GO:0051085", "GO:0140662", "GO:1990220" ] }, "pscc": { "uniref50_id": "UniRef50_P47632", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47632" ], "product": "Chaperonin GroEL" }, "genes": [ "groL" ], "id": "JPCNHPFPBM_901", "locus": "GCA000292505_00901" }, { "type": "cds", "sequence": "contig_1", "start": 496117, "stop": 496449, "strand": "-", "frame": 1, "gene": "groES", "product": "co-chaperone GroES", "db_xrefs": [ "COG:COG0234", "COG:O", "GO:0005524", "GO:0005737", "GO:0044183", "GO:0046872", "GO:0051082", "GO:0051085", "GO:0051087", "RefSeq:WP_010869464.1", "SO:0001217", "UniParc:UPI0000127634", "UniRef:UniRef100_P47633", "UniRef:UniRef50_P47633", "UniRef:UniRef90_P47633" ], "nt": "ATGAACATAACGCCAATTCATGACAACGTCTTGGTTTCACTTGTGGAATCAAACAAAGAAGAAGTCTCAAAAAAAGGGATTATTACCTCATTGGCAAGTAATGATAAAAGCGATGCTAATGCTAATAAAGGGATTGTAATTGCTCTTGGTGCTGGTCCTGCATATGGCAAAACAGAAAAACCAAAATATGCTTTTGGTGTTGGTGATATTATTTACTTTAAGGAGTATAGTGGTATCTCTTTTGAGAATGAGGGAAACAAGTACAAAATTATTGGATTTGAGGATGTACTTGCCTTTGAAAAACCAGAAAGTGGTAAGCAAAGAAAAAGATAA", "aa": "MNITPIHDNVLVSLVESNKEEVSKKGIITSLASNDKSDANANKGIVIALGAGPAYGKTEKPKYAFGVGDIIYFKEYSGISFENEGNKYKIIGFEDVLAFEKPESGKQRKR", "aa_hexdigest": "00e270f14d81d7b2ac3f76a93d713102", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000127634", "ncbi_nrp_id": "WP_010869464.1", "uniref100_id": "UniRef100_P47633", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000127634", "RefSeq:WP_010869464.1", "UniRef:UniRef100_P47633" ] }, "ips": { "uniref100_id": "UniRef100_P47633", "uniref90_id": "UniRef90_P47633" }, "psc": { "uniref90_id": "UniRef90_P47633", "gene": "groES", "product": "co-chaperone GroES", "uniref50_id": "UniRef50_P47633", "cog_id": "COG0234", "cog_category": "O", "go_ids": [ "GO:0005737", "GO:0005524", "GO:0046872", "GO:0044183", "GO:0051087", "GO:0051082", "GO:0051085" ] }, "pscc": { "uniref50_id": "UniRef50_P47633", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47633" ], "product": "Co-chaperonin GroES" }, "genes": [ "groES" ], "id": "JPCNHPFPBM_902", "locus": "GCA000292505_00902" }, { "type": "cds", "sequence": "contig_1", "start": 496575, "stop": 496901, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00464.25" ], "nt": "GTGTCAAAAGGTACTGAAACCCACTTATTTTCATTAGTGGTTGGTAATGGTAAAGATGTTGCGTTGTGGTTACAAAAAGCTAACATTGTTTTGAATATGAATACAATCCCTTTTGAAACAAAATCTGCTTTTAGTCCTTCAGGTATTAGACTTGGAACTCCTGCAATGACAACCAGAGGTTTTAAAACTAATGACTTTATTTTTGTTGCCAGTTTGATTGATAAGGTTATTAAAAGTAATGGTAATCAAAAGGTAATTAGTCAAACAAAAACAGCTGTTTTAAATCTCTTAAAACGCTTTCCGCTCTATAAGGGTTTAGCTTATTAA", "aa": "MSKGTETHLFSLVVGNGKDVALWLQKANIVLNMNTIPFETKSAFSPSGIRLGTPAMTTRGFKTNDFIFVASLIDKVIKSNGNQKVISQTKTAVLNLLKRFPLYKGLAY", "aa_hexdigest": "316b9f1848202e548b155262d8a54a9e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00464.25", "name": "SHMT", "length": 76, "aa_cov": 0.6666666666666666, "hmm_cov": 0.9868421052631579, "evalue": 1.1690356808966035e-17, "score": 58.72493362426758, "start": 2, "stop": 73 } ], "seq_stats": { "molecular_weight": 11863.782799999984, "isoelectric_point": 10.197689628601072 }, "id": "JPCNHPFPBM_903", "locus": "GCA000292505_00903" }, { "type": "cds", "sequence": "contig_1", "start": 496926, "stop": 497795, "strand": "-", "frame": 2, "gene": null, "product": "Serine hydroxymethyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2E8WS34" ], "nt": "ATGTTTTCAAAGGTAAGACTTTTACTTAATAAAGAGTTACAACGTCAAAGAGAAAACATTTGTTTAATTGCTTCAGAAAATTACGTTAGCCAAGACATATTAGCTGTAACTGGTTCAGTATTAACAAATAAATATGCAGAAGGCTATCCCAGTAAACGTTTTTATCAAGGCTGTGAAGTTGTTGATGAATCTGAAAACTTAGCCATTGAAAGTTGCAAAACTTTATTTGGAGCACAATGGGCTAATGTTCAACCTCATTCTGGATCATCTGCTAACTATGCAGTTTACTTAGCATTATTAAAACCAGGAGATACTATCTTAGGATTAGATCTTAATTGTGGTGGTCATTTAACCCATGGTAGCCCCGTTAATTTTTCAGGTAAGCAATATCAAGCAGTAACTTATTCGTTAGATTTTGAAACAGAAACTCTTGATTATGATGCAATTCTTCAAATTGCTATTGAACACAAACCAAAGCTAATTATTTGTGGTTTTTCTAACTATTCTAGGACTGTTGACTTTAAAAAATTTAGTGCAATTGCAAAACAAGTTAATGCGTATCTTTTAGCTGATATTGCCCATATTGCTGGTTTCATCGCTGCAGGTTTGCACCAAAACCCTTTGCCTTTTGTGGATGTTGTCACTTCAACAACTCATAAAACTTTGCGTGGTCCTAGGGGGGGTATCATTATGTCTAACAACCAAGCAATTATCAAAAAACTTGATAGTGGAGTATTTCCTGGATGTCAGGGTGGACCTTTACAACATGTGATAGCAGCTAAATATGTTTGTTTTAAAGAAGCTTTGAATCCAAAGTTTAAGCAGTATATGCAACAAGTTAAAGATAATGCTTTAGCAATGGCAAATTGA", "aa": "MFSKVRLLLNKELQRQRENICLIASENYVSQDILAVTGSVLTNKYAEGYPSKRFYQGCEVVDESENLAIESCKTLFGAQWANVQPHSGSSANYAVYLALLKPGDTILGLDLNCGGHLTHGSPVNFSGKQYQAVTYSLDFETETLDYDAILQIAIEHKPKLIICGFSNYSRTVDFKKFSAIAKQVNAYLLADIAHIAGFIAAGLHQNPLPFVDVVTSTTHKTLRGPRGGIIMSNNQAIIKKLDSGVFPGCQGGPLQHVIAAKYVCFKEALNPKFKQYMQQVKDNALAMAN", "aa_hexdigest": "9516b19e5e8c419d4cd1d46a575f28ce", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0025FA9D98", "query_cov": 0.972318339100346, "subject_cov": 0.8593272171253823, "identity": 0.609, "score": 361.0, "evalue": 2.45e-122, "valid": false, "product": "serine hydroxymethyltransferase", "ec_ids": [ "2.1.2.1" ], "uniref50_id": "UniRef50_A0A2E8WS34", "kegg_orthology_id": "K00600" }, "pscc": { "uniref50_id": "UniRef50_A0A2E8WS34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2E8WS34" ], "product": "Serine hydroxymethyltransferase" }, "genes": [], "id": "JPCNHPFPBM_904", "locus": "GCA000292505_00904" }, { "type": "cds", "sequence": "contig_1", "start": 497805, "stop": 498509, "strand": "-", "frame": 2, "gene": null, "product": "putative lipoprotein MG395", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47635", "UniRef:UniRef90_P47635" ], "nt": "TTGGGTTATCCTGTACTTGAAAGAGGTTATGATCAAAACCGCTTATTAGAACAAAAAGAAGAGTTTACTAGAACTCATAACTTTAATCAAAAAAGTCAGTTATGGAGTAAAAACACATATTTAGTTTCAACTGCTAAAGAGATCCCTGTAATTACAAAAAATGCCAGAAAAGATGGCCAAATAGGTAGTGAAATATTTTCCAAAAAACCCCAAGACAGTCATGTTGATAAAGTTATTAAACACGAAAAAGGGATTGTTACATTTCAAAACTTCAAAAACTTTAAACTTAAATATCATGACAAAGAGTACCAACAGTATGGATATGGTTTAATGCTTGATGATACCAATCTCCCTGGTGGTTCTTCTGGAAGTGCTATATTTAACAACAACCAAAAAATTAACAGTATTTATTTTGGTGTTTTAGAGGTATATAAGAATTCCAAAACACATAAAGATAACATTGGTATGTCTCAACTTTTAAGAACTCCTAAACGTGAAAGTGAAAAAAATAAAACCAGAACTAATAATAGGGATAAATTTCAACACTATGATTTAATTTTTGGTGACAGTAACACAAAATCTTTTTATGCACAATTTGCTAAAAAGCACAATACCCACTTGTACGACCAAATTAAAAGTAGTGAAAAGGAAGAATTTAAATATGTTGATAAGAACAGTCAAAAAACACCGTTTTTATTACGTTAA", "aa": "MGYPVLERGYDQNRLLEQKEEFTRTHNFNQKSQLWSKNTYLVSTAKEIPVITKNARKDGQIGSEIFSKKPQDSHVDKVIKHEKGIVTFQNFKNFKLKYHDKEYQQYGYGLMLDDTNLPGGSSGSAIFNNNQKINSIYFGVLEVYKNSKTHKDNIGMSQLLRTPKRESEKNKTRTNNRDKFQHYDLIFGDSNTKSFYAQFAKKHNTHLYDQIKSSEKEEFKYVDKNSQKTPFLLR", "aa_hexdigest": "6bfe31d1a2a1f8e33522b05298a2b7be", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 497805, "stop": 498809, "observations": { "mutation": [ 498530, 498629 ], "stop": [ 498530, 498629 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47635", "query_cov": 1.0042735042735043, "subject_cov": 0.4484732824427481, "identity": 0.9740000000000001, "score": 447.0, "evalue": 2.02e-154, "gene_start": 1, "gene_end": 234, "reference_start": 290, "reference_end": 524, "reference_sequence": "MLKIDMWFKLKSLGFSLISLQALLASCSAVSPVPVPIEEKNDSTTDNNATPFKDEQSDQGTEVNQQPKVEQKVYNRNFKFNTSFIPEESDIYRKGYDLTFTLNFTSFSNDSYGTGWLIDWKGDENTTQKQGSFFAYIATNLHVADGLRNIGDHWPYSKTDDQREFNEYESTVYFSIGKYTNKTDITKLYQEEKLEQRKVNDSLLSIQTSNIPKTAYTATNFLKGVNSIKPVYADFAVIELELNLENLRDWQIFNEFIKPAINTYKSLGDSTNIFETKDLEQHWNHSHYLLGYPVLERGYDQNRLLEQKEEFARTHNFNQKSQLWSKNTYLVSTAKEIPVITKNARKDGQIGSEIFSKKTQDSHVDKVIKHEKGIVTFQNFKNFKLKYHDKEYQQYGYGLMLDDTNLPGGSSGSAIFNNNQKINSIYFGVLEVYKNSKTHKDNIGMSQLLRTSKRESEKNKTRTTNNRDKFQHYDLIFGDSNTKSFYAQFAKKHNTHLYDQIKSSEKEEFKYVDKNNQKTPFLLR" }, "paralog": false, "identity": 0.9791044776119403, "score": 656.0, "evalue": 4.62e-239, "description": "Internal stop codon at 498530, 498629. Nonsense mutation around 498530, 498629.", "psc": { "uniref90_id": "UniRef90_P47635", "product": "Uncharacterized lipoprotein MG395", "uniref50_id": "UniRef50_P47635", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47635", "UniRef:UniRef50_P47635", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47635", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47635" ], "product": "Uncharacterized lipoprotein MG395" } }, "id": "JPCNHPFPBM_905", "locus": "GCA000292505_00905" }, { "type": "cds", "sequence": "contig_1", "start": 499029, "stop": 499376, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAAGATTGATATGTGGTTTAAACTAAAAAGTTTAGGTTTTTCACTAATTTCATTACAAGCACTTTTAGCTTCTTGTTCTGCTGTTTCACCTGTTCCTGTTCCTATAGAAGAAAAAAACGACAGCACAACAGATAACAACGCTACTCCTTTTAAAGATGAACAATCTGGTCAAGGAACAGAAGTAAATCAGCAACCTAAGGTAGAACAGAAGGTTTACAACAGAAACTTTAAATTTAATACAAGTTTTATCCCAGAAGAAAGTGATATATACAGAAAAGGATATGATTTAACTTTTACTTTAAATTTCACTAGCTTTAGTAATGACTCTTATGGTACTGGTTGA", "aa": "MLKIDMWFKLKSLGFSLISLQALLASCSAVSPVPVPIEEKNDSTTDNNATPFKDEQSGQGTEVNQQPKVEQKVYNRNFKFNTSFIPEESDIYRKGYDLTFTLNFTSFSNDSYGTG", "aa_hexdigest": "79434b69ad0c92fcba6b418d3b58bdce", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12948.290599999986, "isoelectric_point": 4.848388862609862 }, "id": "JPCNHPFPBM_906", "locus": "GCA000292505_00906" }, { "type": "cds", "sequence": "contig_1", "start": 499537, "stop": 499668, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACTAATGAAAGCAAATGCAACCATCAAAAAAGTTAAAAATGAAAATAGAACTACTACTGACTCAGGTAAACTAACTAAATTAAAACCAAAAAAATTAATCCCGTTAGAATTTGCATTGGCTTGA", "aa": "MKKLMKANATIKKVKNENRTTTDSGKLTKLKPKKLIPLEFALA", "aa_hexdigest": "3d99db78dbb2cdeac1708e02e28e014a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4841.8672, "isoelectric_point": 10.420943641662596 }, "id": "JPCNHPFPBM_907", "locus": "GCA000292505_00907" }, { "type": "cds", "sequence": "contig_1", "start": 499747, "stop": 500205, "strand": "-", "frame": 1, "gene": "rpiB", "product": "ribose 5-phosphate isomerase B", "db_xrefs": [ "COG:COG0698", "COG:G", "EC:5.3.1.6", "GO:0004751", "GO:0009052", "GO:0019316", "RefSeq:WP_009885626.1", "SO:0001217", "UniParc:UPI000013973C", "UniRef:UniRef100_P47636", "UniRef:UniRef50_P47636", "UniRef:UniRef90_P47636" ], "nt": "ATGAGTTTTAATATCTTTATTGCTTCTGATCATACCGGTTTAACTCTTAAAAAAATTATTAGTGAACACCTAAAGACTAAGCAGTTTAATGTAGTTGATCTTGGCCCTAATTATTTTGATGCTAATGATGATTATCCTGACTTTGCTTTTTTGGTAGCTGATAAGGTCAAGAAAAATTCTGATAAAGATCTTGGGATACTAATTTGCGGTACTGGTGTTGGGGTTTGTATGGCAGCAAATAAAGTAAAAGGAGTATTAGCGGCTTTGGTTGTTAGTGAGAAAACAGCAGCTTTAGCTAGACAACATGATAATGCCAATGTTTTATGTCTTTCCAGTCGTTTTGTAACTGACAGTGAAAACATTAAAATAGTGGATGATTTTCTCAAAGCTAATTTTGAAGGTGGTAGACACCAAAGACGGATAGATAAGATTATTCGATATGAAAAAGAAACTGAATAG", "aa": "MSFNIFIASDHTGLTLKKIISEHLKTKQFNVVDLGPNYFDANDDYPDFAFLVADKVKKNSDKDLGILICGTGVGVCMAANKVKGVLAALVVSEKTAALARQHDNANVLCLSSRFVTDSENIKIVDDFLKANFEGGRHQRRIDKIIRYEKETE", "aa_hexdigest": "36c336623729202195316291c6d57640", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013973C", "ncbi_nrp_id": "WP_009885626.1", "uniref100_id": "UniRef100_P47636", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013973C", "RefSeq:WP_009885626.1", "UniRef:UniRef100_P47636" ] }, "ips": { "uniref100_id": "UniRef100_P47636", "uniref90_id": "UniRef90_P47636" }, "psc": { "uniref90_id": "UniRef90_P47636", "gene": "rpiB", "product": "ribose 5-phosphate isomerase B", "ec_ids": [ "5.3.1.6" ], "uniref50_id": "UniRef50_P47636", "cog_id": "COG0698", "cog_category": "G", "go_ids": [ "GO:0004751", "GO:0009052", "GO:0019316" ] }, "pscc": { "uniref50_id": "UniRef50_P47636", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47636" ], "product": "Probable ribose-5-phosphate isomerase B" }, "genes": [ "rpiB" ], "id": "JPCNHPFPBM_908", "locus": "GCA000292505_00908" }, { "type": "cds", "sequence": "contig_1", "start": 500206, "stop": 500973, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MG397", "db_xrefs": [ "GO:0000921", "GO:0005940", "GO:0016020", "SO:0001217", "UniRef:UniRef50_P47637", "UniRef:UniRef90_P47637" ], "nt": "GTGGTTGAAAATATTGATGATTTTCGTAATCAAATTCAACAATATGAAAAGAAAAATGAGCTTTTAAAAACAACAATTAAAGCCATTGAATTAGTGTTCCAAAATGAAACAGATGTGCATGATTTAATTATTGAGCTTAATGAGAACTGCGATCTAATTACTAAAAATATTAATAACTTAAACCATCAAAGTTTAGCTCAGAATCAAATCAATTATGAAAAACTGTTTTACTTGATGAGTCAAACAAATCAAGCTTTAGAAAAACTGAAAAATTCACTAACTGATTTACTTAATTTAGCAGTTAATAAGTTTGACGACTATCGCTTTTTTGTCTATACTTTAGATGATTTCCGATTTAAATTTTTTCAGATTGAAAATTTAATTAGCGATCAAGATTTAAAGGTTCCTGCTAAAACATTGCAAGTAATTAATAAATCGAAATTAGAACTTGATAACATCTTCACTGAAGCTAATAATAACTATTTAGGTACTTTTAATTTGTTTAGTGAAAAGATAGATTTATTGCAAATTCAATTGACTGAAGTTATTTGTGATGTTGTTAGTTTAGTTAACTTAAGGCTAATGTGCAAACAAGCTTTTCTTTTTGCAAATAAATATCGTCAAAAGAGTAGTCATATCGATAGATCATTAGAACAGTTAACAAAACTATACAATCAACAAAACTATCCTGATACATTAGAAGGATTAATTAATCTTTTAACTAAAATTAAAACCAATACAAAACAAGACTATCTGGAACTGAATTAA", "aa": "MVENIDDFRNQIQQYEKKNELLKTTIKAIELVFQNETDVHDLIIELNENCDLITKNINNLNHQSLAQNQINYEKLFYLMSQTNQALEKLKNSLTDLLNLAVNKFDDYRFFVYTLDDFRFKFFQIENLISDQDLKVPAKTLQVINKSKLELDNIFTEANNNYLGTFNLFSEKIDLLQIQLTEVICDVVSLVNLRLMCKQAFLFANKYRQKSSHIDRSLEQLTKLYNQQNYPDTLEGLINLLTKIKTNTKQDYLELN", "aa_hexdigest": "e3916977cbe7eb7316d982ef58945201", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 500206, "stop": 501273, "observations": { "mutation": [ 501018 ], "stop": [ 501018 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47637", "query_cov": 1.0, "subject_cov": 0.450530035335689, "identity": 0.992, "score": 486.0, "evalue": 8.98e-169, "gene_start": 1, "gene_end": 255, "reference_start": 312, "reference_end": 566, "reference_sequence": "MLVFLILLPFLLLVIFGAYLLVCLFVRQNNLLLKKTKQLYNNLKLSNFNCVISPFQLLRNEKQALEQSIKVLKQFQIKFDNEINSAFEQLTVYSKPNNIYRFIWFNRNIKLLNKNLQHLYTKQQEYINLTKNAIVYFNNSYDCLVFYRQAFCFLNQFINNFLIEKYDSLFYLNVLNRISLLFKEIENCIQAKDVEKQIAFLNKLHNILSQTIITANRQYMFDIKLSYLTHHFKVLIQKVKQMELMKNKVINSQDLLKLHELINNITIKLKDCTNFLNNLNLMLCERNMDEVQEAISWLFQAVSSKEKSINLVVENIDDFRNQIQQYEKKNELLKTTIKAIELVFQNETDVHDLIIELNENCDLITKNINNLNHQSLAQNQINYEKLFYLMSQTNQALEKLKNSLTDLLNLAVNKFDDYRFFVYTLDDFRFKFFQIENLISDQDLKVPAKTLQVINKSKLELDNIFTEANNNYLGTFNLFSEKIDLLQIQLTEVICDVVSLVNLRLMCKQAFLFANKYRQKSSHIDRSLEQLTKLYNRQNYPDTLEGLINLLTKIKTNTKQDYLELN" }, "paralog": false, "identity": 0.9915492957746479, "score": 689.0, "evalue": 3.99e-251, "description": "Internal stop codon at 501018. Nonsense mutation around 501018.", "psc": { "uniref90_id": "UniRef90_P47637", "product": "Uncharacterized protein MG397", "uniref50_id": "UniRef50_P47637", "go_ids": [ "GO:0016020", "GO:0005940", "GO:0000921" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47637", "UniRef:UniRef50_P47637", "GO:0016020", "GO:0005940", "GO:0000921" ] }, "pscc": { "uniref50_id": "UniRef50_P47637", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47637" ], "product": "Uncharacterized protein MG397" } }, "id": "JPCNHPFPBM_909", "locus": "GCA000292505_00909" }, { "type": "cds", "sequence": "contig_1", "start": 501016, "stop": 501366, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAAAGAGATAGAAAATTGTATTCAAGCTAAAGATGTAGAAAAGCAAATTGCTTTTTTAAACAAATTACATAACATTCTCAGTCAAACAATTATTACTGCTAACAGGCAATATATGTTTGATATTAAGTTAAGTTATTTAACCCATCACTTTAAGGTTTTAATTCAAAAGGTAAAACAAATGGAATTAATGAAAAATAAAGTAATTAACAGTCAAGATCTCTTAAAACTTCATGAATTGATTAATAACATTACCATTAAATTGAAGGATTGTGCCAATTTTCTTAATAACTTAAATTTAATGCTATGCGAAAGGAATATGGATGAAGTTCAAGAGGCGATTAGTTGA", "aa": "MFKEIENCIQAKDVEKQIAFLNKLHNILSQTIITANRQYMFDIKLSYLTHHFKVLIQKVKQMELMKNKVINSQDLLKLHELINNITIKLKDCANFLNNLNLMLCERNMDEVQEAIS", "aa_hexdigest": "efc1a90f92b00c6fdd141249062c94f1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13706.059999999985, "isoelectric_point": 8.439765739440919 }, "id": "JPCNHPFPBM_910", "locus": "GCA000292505_00910" }, { "type": "cds", "sequence": "contig_1", "start": 501595, "stop": 501906, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGGTTTTTCTAATCTTGCTCCCTTTCTTATTACTAGTAATTTTTGGTGCTTATCTATTAGTTTGTCTTTTTGTTAGACAAAATAACTTACTTTTAAAAAAAACCAAACAACTCTATAATAACTTGAAATTAAGCAATTTCAATTGTGTGATTAGTCCATTTCAATTACTAAGAAATGAAAAACAAGCGTTAGAACAATCCATTAAAGTTTTAAAACAGTTTCAAATCAAATTTGATAATGAAATCAATTCAGCTTTTGAGCAACTAACAGTTTACAGTAAACCAAATAATATTTATCGTTTCATCTGA", "aa": "MLVFLILLPFLLLVIFGAYLLVCLFVRQNNLLLKKTKQLYNNLKLSNFNCVISPFQLLRNEKQALEQSIKVLKQFQIKFDNEINSAFEQLTVYSKPNNIYRFI", "aa_hexdigest": "8ed337e99d3e8149333b4e559996cda4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12188.453599999986, "isoelectric_point": 9.697221565246583 }, "id": "JPCNHPFPBM_911", "locus": "GCA000292505_00911" }, { "type": "cds", "sequence": "contig_1", "start": 501908, "stop": 502309, "strand": "-", "frame": 3, "gene": "atpC", "product": "F0F1 ATP synthase subunit epsilon", "db_xrefs": [ "COG:C", "COG:COG0355", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0045261", "GO:0046933", "RefSeq:WP_010869467.1", "SO:0001217", "UniParc:UPI00001264F9", "UniRef:UniRef100_P47638", "UniRef:UniRef50_P47638", "UniRef:UniRef90_P47638" ], "nt": "ATGAAGTTATTGCGCTTTTTGGTACTTAGTCCTAGTGGCATAAAACTAGATAAAACCATTATTAGTGCGCAAGTTAAAACTACTGAAGGTTACATAGGATTAAATTTTAATCGCGCTCCTTTGATTGCTGCTATTCAATCCCATCTGTGCAAAATTATTTTTGCTGATCAAACAAAAAGAGAAGCAATTATTGGTGCTGGTTTAATGCTTATTAAAAAAACAGAAGCTAAGATTTTCACAGAAAATTTTGTTTTTGCTGATGAAGTTGATATTAATGAAACCTTAAAAAGAAAAACAGAACTTGAAAGAAAAATTCACCATATCAAGGATGCTAAGCTAAACGTTAAAATTGAACAAAATTTAATGTTTGAACTATTAAAACTTTCAAGTAAGAAAAAATAA", "aa": "MKLLRFLVLSPSGIKLDKTIISAQVKTTEGYIGLNFNRAPLIAAIQSHLCKIIFADQTKREAIIGAGLMLIKKTEAKIFTENFVFADEVDINETLKRKTELERKIHHIKDAKLNVKIEQNLMFELLKLSSKKK", "aa_hexdigest": "9e3ba339b581709bd5a4fab56172c3fa", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001264F9", "ncbi_nrp_id": "WP_010869467.1", "uniref100_id": "UniRef100_P47638", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001264F9", "RefSeq:WP_010869467.1", "UniRef:UniRef100_P47638" ] }, "ips": { "uniref100_id": "UniRef100_P47638", "uniref90_id": "UniRef90_P47638" }, "psc": { "uniref90_id": "UniRef90_P47638", "gene": "atpC", "product": "F0F1 ATP synthase subunit epsilon", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47638", "cog_id": "COG0355", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0045261", "GO:0005524", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P47638", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47638" ], "product": "ATP synthase epsilon chain" }, "genes": [ "atpC" ], "id": "JPCNHPFPBM_912", "locus": "GCA000292505_00912" }, { "type": "cds", "sequence": "contig_1", "start": 502314, "stop": 503744, "strand": "-", "frame": 2, "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "db_xrefs": [ "COG:C", "COG:COG0055", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0016887", "GO:0045261", "GO:0046933", "GO:0046961", "SO:0001217", "UniRef:UniRef50_P50002", "UniRef:UniRef90_Q50331" ], "nt": "TTGATAAAAAAAGAAAACCTAACATATGGTAAAGTTCACCAAGTCATTGGTCCTGTAGTTGATGTTATCTTTTCAGAAAGTAAACAATTACCTAGAGTTTATGATTGTTTGAGTGTACAACTAGAAAAAAGTGAGCTTTTTTTAGAAGCAACCCAATTAATAGGTGATGACATTGTTCGTTGCATTGCATTAGGTCCTACAGAAGGATTAGCACGTAATGTTAAAGTTACTAACTATAACCATCCAATAGAGGTACCTGTTGGCAAAAATGTATTGGGAAGGATGTTCAATGTTTTAGGTGAACCCATTGATGGAAAAGAACCATTACCAAAAAAACCAAAGCTATCAATCCATCGTAACCCACCTACTTTTGATGAACAACCAAATACTGTTGATATTTTTGAAACAGGAATAAAAGTAATTGATCTTTTAACTCCTTACGTTAGGGGGGGTAAAATTGGTTTATTTGGAGGAGCTGGTGTTGGGAAAACTGTTTTGGTGCAAGAATTAATTCATAACATTGCCAAAGAACATTCTGGTTTAAGTGTATTTGCTGGAGTTGGTGAAAGAACAAGAGAAGGTAATGATCTTTACTATGAAATGATTCAAGGTGGGGTGATTGATAAAACAGCTTTAGTTTTTGGCCAAATGAATGAACCACCAGGAGCTAGAATGAGAGTTGCTTTAACTGCTTTAACAATGGCAGAATATTTTCGTGATCATGATAATCAGAATGTGCTGTTATTCATTGACAATATTTTTCGTTTTACTCAAGCAGGTAGTGAGGTTTCAGCATTACTTGGTAGAATGCCATCTGCTGTTGGCTATCAACCAACTTTAGCTATTGAAATGGGTAAGTTACAAGAAAGAATTGCTTCTACCAAAACAGGTTCTATTACATCTGTTCAAGCTATCTATGTTCCAGCAGATGATCTAACAGACCCAGCACCTGCAACAACATTTACCCATCTTGATGCTAAAACAGTGTTGGATCGTAATATTGCAGCACTAGGTATTTTTCCAGCAATTAATCCTTTAGAATCAACAAGTCGTTTATTAGATCCTAGTGTTGTTGGTATCAACCATTATAAAGTCGCTTTAGGAGTGCAAAATATCTTGCAGCGTTTTGCAGAATTACAAGATATCATTGCTATACTAGGGATTGATGAATTGTCTGATGAAGATAAGATTATTGTTGAAAGAGCAAGAAGGATACGTAACTTTTTATCCCAACCTTTTTTTGTTGCTGAAAAGTTTTCAGGTATTGCAGGTAAATATGTATCTTTAAATGATACTGTTCAATCTTTTAAAGAAATTTTGGAAGGTAAGCATGATCATTTGCCTGAACAAGCATTCTTTTATGTTGGAACCATTCAAGAGGCTGTTGAAAAAGCAAAAAGATTAAATCAAGAGTTTGATAAAACTAAATAG", "aa": "MIKKENLTYGKVHQVIGPVVDVIFSESKQLPRVYDCLSVQLEKSELFLEATQLIGDDIVRCIALGPTEGLARNVKVTNYNHPIEVPVGKNVLGRMFNVLGEPIDGKEPLPKKPKLSIHRNPPTFDEQPNTVDIFETGIKVIDLLTPYVRGGKIGLFGGAGVGKTVLVQELIHNIAKEHSGLSVFAGVGERTREGNDLYYEMIQGGVIDKTALVFGQMNEPPGARMRVALTALTMAEYFRDHDNQNVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLAIEMGKLQERIASTKTGSITSVQAIYVPADDLTDPAPATTFTHLDAKTVLDRNIAALGIFPAINPLESTSRLLDPSVVGINHYKVALGVQNILQRFAELQDIIAILGIDELSDEDKIIVERARRIRNFLSQPFFVAEKFSGIAGKYVSLNDTVQSFKEILEGKHDHLPEQAFFYVGTIQEAVEKAKRLNQEFDKTK", "aa_hexdigest": "bc4d402d08eb2cd2d23a42359156f9e6", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q50331", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.9940000000000001, "score": 928.0, "evalue": 0.0, "valid": true, "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P50002", "cog_id": "COG0055", "cog_category": "C", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0016887", "GO:0045261", "GO:0046933", "GO:0046961" ] }, "pscc": { "uniref50_id": "UniRef50_P50002", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P50002" ], "product": "ATP synthase subunit beta, sodium ion specific" }, "genes": [ "atpD" ], "id": "JPCNHPFPBM_913", "locus": "GCA000292505_00913" }, { "type": "cds", "sequence": "contig_1", "start": 503746, "stop": 504141, "strand": "-", "frame": 1, "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "db_xrefs": [ "COG:C", "COG:COG0224", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933", "SO:0001217", "UniRef:UniRef50_P47640", "UniRef:UniRef90_P47640" ], "nt": "ATGGATGCATTTAAAGAGTTTAAACTTGATCGAATTTGTATGGTTTACACTAAATTTAAAAACTCATTAATCCAACAATCTCAGCTCTTTCAAGTTTTTCCTTTCGATGTTGAAACTTTTAAAACTTTAAATCCGGTTGTAACTGATCAACAACTTGATTTTGAGCCAGATCAAGCCACGATAATTAATTTAATTACTCCACAGTTTTTTGATGTGGCTCTGTATGGTGGCCTTGTTGAAACTAAGTTATGTGAATCAGCTTCTAGACAAAATGCAATGGAAGCTGCTACAAAGAATGCTAAAGATTTACTTGATAAATACACTTTACAATTTAACAAGCTAAGACAAAACTCTATTACAGAAGAGATTATTGAAGTTATAGGAGGTATGAATTAA", "aa": "MDAFKEFKLDRICMVYTKFKNSLIQQSQLFQVFPFDVETFKTLNPVVTDQQLDFEPDQATIINLITPQFFDVALYGGLVETKLCESASRQNAMEAATKNAKDLLDKYTLQFNKLRQNSITEEIIEVIGGMN", "aa_hexdigest": "b53748a57bc025a0916f30df669a8818", "start_type": "ATG", "rbs_motif": null, "genes": [ "atpG" ], "truncated": "3-prime", "pseudogene": { "start": 503746, "stop": 504441, "observations": { "mutation": [ 504240 ], "stop": [ 504240 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47640", "query_cov": 1.0, "subject_cov": 0.46953405017921146, "identity": 1.0, "score": 259.0, "evalue": 1.23e-85, "gene_start": 1, "gene_end": 131, "reference_start": 149, "reference_end": 279, "reference_sequence": "MAFIQEIKRRMNTVKSTIKITNAMKMVSRAKFIKFKKQFQEISLFFNEFYKAVGQVVVSLKEPKKKPDNQKTLWIMMSSSLGLCGQHNSNMNKLLKANFKADDKIFFLGRKNQSFWNKNSQYNPAVGFIDIQDRDINFDYCQTIFDQIMDAFKEFKLDRICMVYTKFKNSLIQQSQLFQVFPFDVETFKTLNPVVTDQQLDFEPDQATIINLITPQFFDVALYGGLVETKLCESASRQNAMEAATKNAKDLLDKYTLQFNKLRQNSITEEIIEVIGGMN" }, "paralog": false, "identity": 0.9956709956709957, "score": 461.0, "evalue": 1.15e-167, "description": "Internal stop codon at 504240. Nonsense mutation around 504240.", "psc": { "uniref90_id": "UniRef90_P47640", "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47640", "cog_id": "COG0224", "cog_category": "C", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47640", "EC:7.1.2.2", "UniRef:UniRef50_P47640", "COG:COG0224", "COG:C", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P47640", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47640" ], "product": "ATP synthase gamma chain" } }, "id": "JPCNHPFPBM_914", "locus": "GCA000292505_00914" }, { "type": "cds", "sequence": "contig_1", "start": 504238, "stop": 504585, "strand": "-", "frame": 1, "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "db_xrefs": [ "COG:C", "COG:COG0224", "EC:7.1.2.2", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933", "SO:0001217", "UniRef:UniRef50_P47640", "UniRef:UniRef90_P47640" ], "nt": "ATGGCTTTTATACAAGAAATTAAGCGCAGAATGAATACAGTAAAATCCACCATTAAGATAACTAATGCAATGAAAATGGTGTCACGCGCTAAGTTTATTAAGTTCAAAAAACAGTTTCAAGAAATTAGTTTGTTTTTTAATGAATTTTATAAAGCTGTTGGCCAAGTAGTTGTTTCTTTAAAAGAACCAAAAAAGAAACCAGATAACCAAAAAACTTTATGGATAATGATGAGTTCTTCTTTAGGACTTTGTGGACAGCATAATTCGAACATGAATAAGTTATTAAAAGCTAATTTTAAAGCTGATGATAAAATCTTTTTTTTAGGTAGAAAAAACCAATCATTTTGA", "aa": "MAFIQEIKRRMNTVKSTIKITNAMKMVSRAKFIKFKKQFQEISLFFNEFYKAVGQVVVSLKEPKKKPDNQKTLWIMMSSSLGLCGQHNSNMNKLLKANFKADDKIFFLGRKNQSF", "aa_hexdigest": "8bfd9797ada4917b2a62320d75c157af", "start_type": "ATG", "rbs_motif": null, "genes": [ "atpG" ], "truncated": "5-prime", "pseudogene": { "start": 503935, "stop": 504585, "observations": { "mutation": [ 504240 ], "stop": [ 504240 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47640", "query_cov": 1.0, "subject_cov": 0.4121863799283154, "identity": 1.0, "score": 224.0, "evalue": 8.42e-72, "gene_start": 1, "gene_end": 115, "reference_start": 1, "reference_end": 115, "reference_sequence": "MAFIQEIKRRMNTVKSTIKITNAMKMVSRAKFIKFKKQFQEISLFFNEFYKAVGQVVVSLKEPKKKPDNQKTLWIMMSSSLGLCGQHNSNMNKLLKANFKADDKIFFLGRKNQSFWNKNSQYNPAVGFIDIQDRDINFDYCQTIFDQIMDAFKEFKLDRICMVYTKFKNSLIQQSQLFQVFPFDVETFKTLNPVVTDQQLDFEPDQATIINLITPQFFDVALYGGLVETKLCESASRQNAMEAATKNAKDLLDKYTLQFNKLRQNSITEEIIEVIGGMN" }, "paralog": false, "identity": 0.9953703703703703, "score": 430.0, "evalue": 1.32e-155, "description": "Internal stop codon at 504240. Nonsense mutation around 504240.", "psc": { "uniref90_id": "UniRef90_P47640", "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47640", "cog_id": "COG0224", "cog_category": "C", "go_ids": [ "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47640", "EC:7.1.2.2", "UniRef:UniRef50_P47640", "COG:COG0224", "COG:C", "GO:0005524", "GO:0005886", "GO:0015986", "GO:0042777", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P47640", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47640" ], "product": "ATP synthase gamma chain" } }, "id": "JPCNHPFPBM_915", "locus": "GCA000292505_00915" }, { "type": "cds", "sequence": "contig_1", "start": 504585, "stop": 505373, "strand": "-", "frame": 2, "gene": null, "product": "ATP synthase alpha chain", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q1RKD9" ], "nt": "TTGATTGTATTTGATGACCTTTCTAAGCATGCTGTTGCTTACAGAACTCTTTCACTCTTGTTAAAAAGACCACCTGGTAGAGAAGCTTTTCCAGGAGATGTTTTTTATTTACATTCAAGACTTTTGGAACGTGCATGCAAGTTAAATGATGAAAATGGTGGTGGCTCAATTACAGCTTTACCAATTATAGAAACTCAAGCTGGTGATATCTCTGCATATATTCCTACAAATGTTATTTCAATTACTGATGGCCAACTGTTTATGGTTAGTAGTCTATTTAACGCTGGACAACGCCCTGCAATTCAAATTGGTTTATCAGTTTCAAGGGTTGGTAGTGCAGCACAAACAAAAGCGATTAAACAGCAAACTGGCAGTTTAAAACTAGAACTTGCTCAGTATAGTGAACTTGATAGTTTTAGTCAATTTGGTAGTGATCTTGATGAAAATACAAAAAAGGTTTTAGAGCATGGTAAAAGAGTAATGGAAATGATTAAACAACCAAATGGTAAACCTTACTCTCAAGTCCATGAAGCATTATTTTTATTTGCTATTAACAAAGCTTTCATTAAGTTTATTCCAGTTGATGAAATTGCTAAATTTAAACAAAGGATAACAGAAGAATTTAATGGTTCCCATCCTCTGTTTAAAGAGTTATCTAACAAAAAAGAATTTACTGAGGATTTAGAAAGTAAAACTAAAACCGCTTTTAAAATGCTTGTGAAACGTTTTATCAGTACATTAACAGATTATGATATTACCAAATTTGGTAGTATTGAGGAACTTAATTAA", "aa": "MIVFDDLSKHAVAYRTLSLLLKRPPGREAFPGDVFYLHSRLLERACKLNDENGGGSITALPIIETQAGDISAYIPTNVISITDGQLFMVSSLFNAGQRPAIQIGLSVSRVGSAAQTKAIKQQTGSLKLELAQYSELDSFSQFGSDLDENTKKVLEHGKRVMEMIKQPNGKPYSQVHEALFLFAINKAFIKFIPVDEIAKFKQRITEEFNGSHPLFKELSNKKEFTEDLESKTKTAFKMLVKRFISTLTDYDITKFGSIEELN", "aa_hexdigest": "8f4965ef61aad525a43c35b53ea2ecc4", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q1RKD9", "query_cov": 0.9351145038167938, "subject_cov": 0.8419243986254296, "identity": 0.58, "score": 279.0, "evalue": 7.85e-91, "valid": false, "gene": "atpA", "product": "F0F1 ATP synthase subunit alpha", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q1RKD9", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_Q1RKD9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q1RKD9" ], "product": "ATP synthase alpha chain" }, "genes": [], "id": "JPCNHPFPBM_916", "locus": "GCA000292505_00916" }, { "type": "cds", "sequence": "contig_1", "start": 505395, "stop": 506141, "strand": "-", "frame": 2, "gene": null, "product": "ATP synthase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9E1" ], "nt": "ATGGCAGATAAACTAAATGAATACGTAGCATTAATCAAAACTGAAATTAAAAAGTATTCCAAAAAAATATTTAACAGTGAAATTGGTCAAGTCATTAGTGTTGCTGATGGAATTGCCAAGGTTAGTGGACTTGAAAATGCTTTATTAAATGAGTTAATTCAATTTGAAAATAATATTCAAGGAATAGTATTAAACCTTGAACAAAATACAGTCGGAATAGCACTTTTTGGTGACTATTCTTCGTTACGAGAAGGCAGTACCGCTAAAAGAACCCACAGTGTAATGAAAACTCCTGTTGGTGATGTTATGCTTGGTAGAATCGTCAATGCACTTGGTGAAGCAATTGATGGTAGAGGTGATATTAAAGCTACTGAATATGATCAAATAGAAAAAATTGCTCCAGGTGTAATGAAAAGGAAAAGTGTTAACCAACCACTTGAAACTGGAATCTTAACAATTGATGCTTTATTTCCTATAGGTAAAGGACAACGTGAATTAATTGTTGGTGATAGACAAACAGGTAAAACTGCTATTGCGATTGACACTATCATTAATCAAAAAGATAAAGATGTTTATTGTGTTTATGTAGCAATTGGTCAAAAAAATTCATCAGTAGCACAAATTGTACACCAACTTGAAGTTAATGATTCAATGAAATACACTACAGTGGTTTGTGCTACAGCTAGTGACTCTGATTCCATGGTTTATTTAAGTCCTTTTACAGGAATAACTATTGCTGAATATTGA", "aa": "MADKLNEYVALIKTEIKKYSKKIFNSEIGQVISVADGIAKVSGLENALLNELIQFENNIQGIVLNLEQNTVGIALFGDYSSLREGSTAKRTHSVMKTPVGDVMLGRIVNALGEAIDGRGDIKATEYDQIEKIAPGVMKRKSVNQPLETGILTIDALFPIGKGQRELIVGDRQTGKTAIAIDTIINQKDKDVYCVYVAIGQKNSSVAQIVHQLEVNDSMKYTTVVCATASDSDSMVYLSPFTGITIAEY", "aa_hexdigest": "f82f3fb9500a29f04daa791e2578ff9f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A223M9E1", "query_cov": 0.9637096774193549, "subject_cov": 0.9372549019607843, "identity": 0.565, "score": 264.0, "evalue": 1.17e-85, "valid": false, "product": "ATP synthase subunit alpha", "uniref50_id": "UniRef50_A0A223M9E1" }, "pscc": { "uniref50_id": "UniRef50_A0A223M9E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9E1" ], "product": "ATP synthase subunit alpha" }, "genes": [], "id": "JPCNHPFPBM_917", "locus": "GCA000292505_00917" }, { "type": "cds", "sequence": "contig_1", "start": 506157, "stop": 506687, "strand": "-", "frame": 2, "gene": "atpH", "product": "ATP synthase F1 subunit delta", "db_xrefs": [ "COG:C", "COG:COG0712", "EC:7.1.2.2", "GO:0005886", "GO:0015986", "GO:0045261", "GO:0046933", "RefSeq:WP_014894691.1", "SO:0001217", "UniParc:UPI00027B3F6C", "UniRef:UniRef100_UPI00027B3F6C", "UniRef:UniRef50_P47642", "UniRef:UniRef90_P47642" ], "nt": "ATGATTAATGCACAAGCATTTGGAACTGCACTTTTTCAATTAAGTGAAGAGCAAAAACAAGTAAAGAAAATTTATGAAGAGTGCCATTTTTTTCTGAAATTAATGCGTAATTTTAAAGATGGTTCATTATCGTTCTTACTTAATTCTTATACACTAACAAAACCAGATAAAATAAGACTTGTTGATAAGTTGTTTAAAAATCATTTTTGTCAAATTTTTGTTGATTTTTTAAAAGTAATTATTTTAAAGGGTTACTTTACTTTAGTTGAACAGGCAATTAAGTATTTTTTTGATAATGTTGAAAGTCAAAAACACATTCAATTTATCAAAATAATTACTGCTTTTGAATTAAGCTCAAAACAACTTAACAAAATTATTGCAATAATGGAAAAACGTTTTAAAACAAAGGTTGTTTATAAAACTGAGATTGATCGCAGTTTAATTTCAGGAATTAGGATAGAATCAAGTTCCCATTTATTTGAAAAAAATGTGCGTGATGAATTAAAACGCATAATGGCCCATTTTATTTAA", "aa": "MINAQAFGTALFQLSEEQKQVKKIYEECHFFLKLMRNFKDGSLSFLLNSYTLTKPDKIRLVDKLFKNHFCQIFVDFLKVIILKGYFTLVEQAIKYFFDNVESQKHIQFIKIITAFELSSKQLNKIIAIMEKRFKTKVVYKTEIDRSLISGIRIESSSHLFEKNVRDELKRIMAHFI", "aa_hexdigest": "23dae4d72ddb8e511dd04a21f54241dc", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B3F6C", "ncbi_nrp_id": "WP_014894691.1", "uniref100_id": "UniRef100_UPI00027B3F6C", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B3F6C", "RefSeq:WP_014894691.1", "UniRef:UniRef100_UPI00027B3F6C" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B3F6C", "uniref90_id": "UniRef90_P47642" }, "psc": { "uniref90_id": "UniRef90_P47642", "gene": "atpH", "product": "ATP synthase F1 subunit delta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47642", "cog_id": "COG0712", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_P47642", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47642" ], "product": "ATP synthase subunit delta" }, "genes": [ "atpH" ], "id": "JPCNHPFPBM_918", "locus": "GCA000292505_00918" }, { "type": "cds", "sequence": "contig_1", "start": 506680, "stop": 506925, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAATTAAAAAGTGAAATAGAAAAAACAGCAAATCGCCAAGCTAACTTAATGATTTTTCAAGCTCGTCAGGAAATTGAAAAAGAAAGACGTTCTCTTAAAGAACAATCTATTAAAGAGAGTGTGGAATTGGCTATGTTGGCTGCACAAGAACTAATTCTCAAGAAAATAGATCAAAAATCAGATAGAGAATTTATTGATAAGTTTATTAGAGATTTAGAAGCTAACGAAACAGAAGATGATTAA", "aa": "MQLKSEIEKTANRQANLMIFQARQEIEKERRSLKEQSIKESVELAMLAAQELILKKIDQKSDREFIDKFIRDLEANETEDD", "aa_hexdigest": "a8c108098cbf6bdcdc66eb92a6854592", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9596.800399999991, "isoelectric_point": 4.959622764587402 }, "id": "JPCNHPFPBM_919", "locus": "GCA000292505_00919" }, { "type": "cds", "sequence": "contig_1", "start": 507309, "stop": 507617, "strand": "-", "frame": 2, "gene": "atpE", "product": "F0F1 ATP synthase subunit C", "db_xrefs": [ "COG:C", "COG:COG0636", "EC:7.1.2.2", "GO:0005886", "GO:0008289", "GO:0015986", "GO:0045263", "GO:0046933", "RefSeq:WP_009885619.1", "SO:0001217", "UniParc:UPI00001265D0", "UniRef:UniRef100_P47644", "UniRef:UniRef50_P47644", "UniRef:UniRef90_P47644" ], "nt": "ATGGAACATGTTAATGAAATTTTAGCTACAGTTGGTGTTATATTACAACAAACTCAAACTACCCAGGATGTTAACGCTAGTGCTAAGCTAGGTGCTTATATAGGTGCTGGTGTTACTATGATTGCAGGTTCAACTGTAGGGATTGGACAAGGTTATATTTTTGGTAAAGCTGTTGAGGCAATAGCAAGAAATCCTGAAGTTGAAAAACAGGTTTTTAAACTAATTTTCATTGGTTCTGCTGTTTCTGAATCTACAGCAATTTATGGACTTTTAATTTCCTTTATCTTAATTTTTGTAGCAGGAGCTTAA", "aa": "MEHVNEILATVGVILQQTQTTQDVNASAKLGAYIGAGVTMIAGSTVGIGQGYIFGKAVEAIARNPEVEKQVFKLIFIGSAVSESTAIYGLLISFILIFVAGA", "aa_hexdigest": "a5fc1b6dc3117211faa233e9c535be74", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001265D0", "ncbi_nrp_id": "WP_009885619.1", "uniref100_id": "UniRef100_P47644", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001265D0", "RefSeq:WP_009885619.1", "UniRef:UniRef100_P47644" ] }, "ips": { "uniref100_id": "UniRef100_P47644", "uniref90_id": "UniRef90_P47644" }, "psc": { "uniref90_id": "UniRef90_P47644", "gene": "atpE", "product": "F0F1 ATP synthase subunit C", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_P47644", "cog_id": "COG0636", "cog_category": "C", "go_ids": [ "GO:0005886", "GO:0045263", "GO:0008289", "GO:0046933", "GO:0015986" ] }, "pscc": { "uniref50_id": "UniRef50_P47644", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47644" ], "product": "ATP synthase subunit c" }, "genes": [ "atpE" ], "id": "JPCNHPFPBM_920", "locus": "GCA000292505_00920" }, { "type": "cds", "sequence": "contig_1", "start": 507620, "stop": 507727, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGACTTATAATTATTGGGCTAAAATGCGCAATCAAGGTTTGGAAAATAATAATGCAAGTGAATTACACTTTAAAGGCATAAAGGTAATTCAAGAAAATATTTAG", "aa": "MLTYNYWAKMRNQGLENNNASELHFKGIKVIQENI", "aa_hexdigest": "e21ecaf31b276c6f44f31206fb2bcdd5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4168.7115, "isoelectric_point": 8.161520195007324 }, "id": "JPCNHPFPBM_921", "locus": "GCA000292505_00921" }, { "type": "cds", "sequence": "contig_1", "start": 507774, "stop": 507875, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGTAATACTGGGGTTATTAAACCTGCAAAAACTGTTCCTAAGCTAAGTGCTAATGGTTGGTTATTAATACTTGAAAAAATAAAAATTCAAAAGTTATAA", "aa": "MCNTGVIKPAKTVPKLSANGWLLILEKIKIQKL", "aa_hexdigest": "59765f25d69fbbb03ccc30b587ed89f7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3649.5006000000003, "isoelectric_point": 10.025236701965333 }, "id": "JPCNHPFPBM_922", "locus": "GCA000292505_00922" }, { "type": "cds", "sequence": "contig_1", "start": 507906, "stop": 508196, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTCCCCATAACCTTAATGAAATAGAAAAAAGCGGTGCAAATCCACTCAATATACTAAAAGGATTTGGAATGAAAGTAGAATACTTTTTTCCTTTAACAGTAATTCCAAAGGCAAACTCTTTAAAAAAATTTCATCTTTGGTATCTAATCCCCATAACAACAATCCCAATAAAAGTTGCAAGTCCTAAAGAAAAAGTAAATGTTAAAGATGATGTTGGTGGTGAAATACCACCAAGCAAGCTAACTAAGTTGCTTGATACTATGTACAGAAGCAACATTAAAAAGTAG", "aa": "MFPHNLNEIEKSGANPLNILKGFGMKVEYFFPLTVIPKANSLKKFHLWYLIPITTIPIKVASPKEKVNVKDDVGGEIPPSKLTKLLDTMYRSNIKK", "aa_hexdigest": "52e48c4e32d4aa890d5b2d0a5b8d4c0d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10895.842099999989, "isoelectric_point": 9.73641834259033 }, "id": "JPCNHPFPBM_923", "locus": "GCA000292505_00923" }, { "type": "cds", "sequence": "contig_1", "start": 508489, "stop": 508668, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCGACATGTTTTCTTTTCGATTTAGATATTAAATTGATAAAAAAATATTCTAAAGGTAACCAAACAAATGATAACACTAAATTTAGTATTGGAAAAAACAATGTTGAAATTATTGTTGCAATAGTGTTAAAAATTGACGGAGTGACAACAAAAGAAATAATCACAGGAATTAAATAA", "aa": "MATCFLFDLDIKLIKKYSKGNQTNDNTKFSIGKNNVEIIVAIVLKIDGVTTKEIITGIK", "aa_hexdigest": "10eba45f6b21e8374251f8065f309968", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6559.688700000001, "isoelectric_point": 9.20764865875244 }, "id": "JPCNHPFPBM_924", "locus": "GCA000292505_00924" }, { "type": "cds", "sequence": "contig_1", "start": 508684, "stop": 508956, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAAAAAATGCAAAGAATGAAACTGCTTTTTGCTTTTTGACATTTTTCAAACTTGCAAAGAAAGATGCTAATCTTAAAACTGATAGTGAATTTAAAACAGCAAAAGGTAATGGCAACATTCAACCAAATAAAATGTCCAAATTATTGCCTTTTGTTAAAACAACAACTGCTATTAAAATTAGTACAAATAATCAAGCTACAAAAATAATTAAGGTAATAATTTTTGCAACCTTTCTATTGTTTCAATTGAACAAAACTTTTAGATGTTAA", "aa": "MKKNAKNETAFCFLTFFKLAKKDANLKTDSEFKTAKGNGNIQPNKMSKLLPFVKTTTAIKISTNNQATKIIKVIIFATFLLFQLNKTFRC", "aa_hexdigest": "ade6e1ebbd4216ea000fa20191d633df", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10211.04439999999, "isoelectric_point": 10.232824897766111 }, "id": "JPCNHPFPBM_925", "locus": "GCA000292505_00925" }, { "type": "cds", "sequence": "contig_1", "start": 508988, "stop": 509362, "strand": "-", "frame": 3, "gene": null, "product": "Enolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A925XKF8" ], "nt": "ATGAACCAATTAACTAAAACCATAGGTAGCCATATTCAAATTGTTGGTGATGACACTTACTGTACTAATGCAGAACTTGCTAAAAAAGGTGTTGCACAAAATACAACAAACTCGATATTGATTAAATTAAATCAAATTGGTTCTATTAGTGAAACGATTCAAACAATTGAAGTTGCAAAAAAAGCTAACTGGAGTCAAGTAATTTCACATCGCAGTGGTGAAACAGAAGATACAACTATTGCTGATTTGGCAGTTGCTGCCCAAACTGGTCAAATTAAAACCGGTTCAATGTCACGCTCAGAAAGAATAGCTAAATACAATCGTTTGTTGTACATAGAAATTGAACTTGGTGATAAAGGAAAATACTTAGGTTGA", "aa": "MNQLTKTIGSHIQIVGDDTYCTNAELAKKGVAQNTTNSILIKLNQIGSISETIQTIEVAKKANWSQVISHRSGETEDTTIADLAVAAQTGQIKTGSMSRSERIAKYNRLLYIEIELGDKGKYLG", "aa_hexdigest": "2117d4f4c162a431da0d04778d0fe3dc", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A1F9NV54", "query_cov": 0.9435483870967742, "subject_cov": 0.9435483870967742, "identity": 0.615, "score": 149.0, "evalue": 4.1e-44, "valid": false, "product": "Enolase", "uniref50_id": "UniRef50_A0A925XKF8" }, "pscc": { "uniref50_id": "UniRef50_A0A925XKF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A925XKF8" ], "product": "Enolase" }, "genes": [], "id": "JPCNHPFPBM_926", "locus": "GCA000292505_00926" }, { "type": "cds", "sequence": "contig_1", "start": 509579, "stop": 510322, "strand": "-", "frame": 3, "gene": null, "product": "Enolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E2U821" ], "nt": "ATGGGAAGTTCAAATCTAAACATCAATTCAAAAATAACCGATATTTTTGCTTATCAAGTTTTTGATTCTCGGGGTGTTCCAACAGTAGCTTGTGTTGTTAAATTGGCATCTGGTCATGTAGGTGAAGCGATGGTTCCATCAGGTGCTTCTACAGGTGAGAAAGAAGCAATTGAATTACGTGATAATGATCCAAAAAATTATTTTGGTAAAGGCGTTAACAAAGCCGTTGATAACGTTAATAAAGTTATTGCCCCTAAGCTTATTGGCTTAAATGCATTTGATCAATTAACAGTGGATCAAGCAATGATTAAACTAGACAATACTCCCAACAAAGCAAAATTAGGAGCAAATGCTATATTATCTGTTTCACTTGCAGTATCAAAAGCAGCAGCAAAAGCACAAAACAGCTCATTATTTCAATACATTTCAAATAAATTAATTGGATTAAATACAACAAATTTTGTTTTACCTGTGCCAATGTTAAATGTAATTAATGGTGGTGCTCATGCTGATAACTATATTGATTTTCAAGAGTTCATGATCATGCCTTTAGGTGCTAAAAAGATGCATGAAGCTTTAAAAATGGCTAGTGAAACTTTTCATGCTTTACAAAATCTTTTAAAAAAGCGTGGATTAAACACAAATAAAGGAGATGAAGGTGGATTTGCGCCTAACTTAAAACTTGCAGAAGATGCACTTGACATCATGGTTGAAGCCATTAAATTAGCTGGATATAAGCCTTGA", "aa": "MGSSNLNINSKITDIFAYQVFDSRGVPTVACVVKLASGHVGEAMVPSGASTGEKEAIELRDNDPKNYFGKGVNKAVDNVNKVIAPKLIGLNAFDQLTVDQAMIKLDNTPNKAKLGANAILSVSLAVSKAAAKAQNSSLFQYISNKLIGLNTTNFVLPVPMLNVINGGAHADNYIDFQEFMIMPLGAKKMHEALKMASETFHALQNLLKKRGLNTNKGDEGGFAPNLKLAEDALDIMVEAIKLAGYKP", "aa_hexdigest": "fd3aef4d3e8750fb02ce38b7343c7fb2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00125F4A82", "query_cov": 0.9595141700404858, "subject_cov": 0.9753086419753086, "identity": 0.586, "score": 257.0, "evalue": 2.89e-83, "valid": false, "product": "phosphopyruvate hydratase", "ec_ids": [ "4.2.1.11" ], "uniref50_id": "UniRef50_A0A9E2U821", "kegg_orthology_id": "K01689" }, "pscc": { "uniref50_id": "UniRef50_A0A9E2U821", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9E2U821" ], "product": "Enolase" }, "genes": [], "id": "JPCNHPFPBM_927", "locus": "GCA000292505_00927" }, { "type": "cds", "sequence": "contig_1", "start": 510859, "stop": 511536, "strand": "-", "frame": 1, "gene": "phoU", "product": "phosphate signaling complex protein PhoU", "db_xrefs": [ "COG:COG0704", "COG:P", "GO:0006817", "GO:0030643", "GO:0045936", "RefSeq:WP_009885614.1", "SO:0001217", "UniParc:UPI0000139775", "UniRef:UniRef100_P47649", "UniRef:UniRef50_P47649", "UniRef:UniRef90_P47649" ], "nt": "ATGGAAAACATTAATTACCAAATTTTAAAGCGTTCAGAAAAGAAATTATTAAAGCTTTTTTTTGAGTATTTTAAGCACGTAATCAATGCACATGAAACATTAAATCAATTACTTTGTGAAGATAATTTAGAAAAACGCAAAGAACTGATTAAAACTATTTATGAAATGGAGGACCAATCTAATAGGTCTGAATTTAAATTAATTAATGAATCAATTTGGACAATCTCTAAAAATTCTCCATTAGCTAGCCATTTACGCCTAACAATCACAATAATTATGTCAAGTCGTGACTTGGAACGCATTTGTGATTATGCTAATAATTTAACTAAGTTTATAGAACATTATTTGCATCTTGATATAAAGATTTTTAATAATCTAGTTACTTTGCATCAAAGTGCGCTCAATAACCTTAAGAAAACCTTTGAAAGTTTACAAGATAAAGAAAAGCCACTTACATTACAATTTGAAAATGCAACCAAAACTATTATTGAATTTGAGCACCAATATCGCCTTGTATTAACAAAATACTATGAAACCATTAATAAAAATGAGGTTACTGAAAGGATTTTCTTAATTGATCTTGTAGCTTCTGTTAAACATATTGAACGGATTAATGATTATTGCTATAACATCATTAAATCCTTTTTATTTATTAAAAATCCTGAAATATTTAATTAG", "aa": "MENINYQILKRSEKKLLKLFFEYFKHVINAHETLNQLLCEDNLEKRKELIKTIYEMEDQSNRSEFKLINESIWTISKNSPLASHLRLTITIIMSSRDLERICDYANNLTKFIEHYLHLDIKIFNNLVTLHQSALNNLKKTFESLQDKEKPLTLQFENATKTIIEFEHQYRLVLTKYYETINKNEVTERIFLIDLVASVKHIERINDYCYNIIKSFLFIKNPEIFN", "aa_hexdigest": "7d23fa54e12868ad1047f677b660b29f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000139775", "ncbi_nrp_id": "WP_009885614.1", "uniref100_id": "UniRef100_P47649", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000139775", "RefSeq:WP_009885614.1", "UniRef:UniRef100_P47649" ] }, "ips": { "uniref100_id": "UniRef100_P47649", "uniref90_id": "UniRef90_P47649" }, "psc": { "uniref90_id": "UniRef90_P47649", "gene": "phoU", "product": "phosphate signaling complex protein PhoU", "uniref50_id": "UniRef50_P47649", "cog_id": "COG0704", "cog_category": "P", "go_ids": [ "GO:0006817", "GO:0030643", "GO:0045936" ] }, "pscc": { "uniref50_id": "UniRef50_P47649", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47649" ], "product": "Uncharacterized protein MG409" }, "genes": [ "phoU" ], "id": "JPCNHPFPBM_928", "locus": "GCA000292505_00928" }, { "type": "cds", "sequence": "contig_1", "start": 511539, "stop": 511883, "strand": "-", "frame": 2, "gene": "pstB", "product": "Phosphate import ATP-binding protein PstB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2H5ZIP6" ], "nt": "GTGAAAGATAATTTACATAGGAATGCAAACACCCTTTCTGGTGGACAACAACAACGTTTGTGTATTGCGCGTGCTATTGCTTTACAACCAGATGTTCTTTTGATGGATGAACCTACCAGTGCTTTAGACTCAATTGCCACAAACTCTATTGAACTTCTAATTCAACAACTAAAAGAAAAATTCACAATTGTTATTGTTACTCACTCTATGGCTCAAACAATTAGAATAACTGATGAAACGATTTTTTTTGCTGATGGAAGAGTAATTGAACAAGGCACTACAAAACAGATATTTACAAAGCCTAAGCAAAAAGCAACAAATAGTTATATAAGTGGGAAAAATTAG", "aa": "MKDNLHRNANTLSGGQQQRLCIARAIALQPDVLLMDEPTSALDSIATNSIELLIQQLKEKFTIVIVTHSMAQTIRITDETIFFADGRVIEQGTTKQIFTKPKQKATNSYISGKN", "aa_hexdigest": "27bc3ce0688b10abfcd82c5b5794130f", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2H5ZIP6", "query_cov": 0.9912280701754386, "subject_cov": 0.9826086956521739, "identity": 0.628, "score": 135.0, "evalue": 6.34e-39, "valid": false, "product": "Phosphate import ATP-binding protein PstB", "uniref50_id": "UniRef50_A0A2H5ZIP6" }, "pscc": { "uniref50_id": "UniRef50_A0A2H5ZIP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2H5ZIP6" ], "product": "Phosphate import ATP-binding protein PstB" }, "genes": [ "pstB" ], "id": "JPCNHPFPBM_929", "locus": "GCA000292505_00929" }, { "type": "cds", "sequence": "contig_1", "start": 511890, "stop": 512027, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTTCAAAAATTAACTCCTTTTAATTTTTCTATTTTTGAAAACATTGCTTATGGCATAAGAGCACATGGTATTCACAATAAAAATGCTATCAATGAAATAGTAAGACAGGCATTGATATCAGCAGCATTGTGA", "aa": "MVFQKLTPFNFSIFENIAYGIRAHGIHNKNAINEIVRQALISAAL", "aa_hexdigest": "3b5a85b6c2cb7493ef7a3d66acb5cbbd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5071.8553999999995, "isoelectric_point": 9.694127082824707 }, "id": "JPCNHPFPBM_930", "locus": "GCA000292505_00930" }, { "type": "cds", "sequence": "contig_1", "start": 512038, "stop": 512454, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGCAATGTTAAATCATTAATAATTCCTGAATTGATATTCTTACCAAGAAAATATATGTCACCTGTTCAACGTGTGTTTTCATTTAAATCATTTAATTTATTTAAGCACCTAATAAAGGTGGATTTACCGCATCCTGATTTACCTATTAAAGCAGTTATTTTATTGCGTTTAATGTCAAGATTGATATCAAATAATACTTGTTTGTTTTTGTTATATCAGAAGTTGAAATTTCGAATTTCAAATACATTTTTTTCATCAAAATCCTTTTTAATGACATTATCTTTGGTGTTTTTAATCTTTATTAGACGGTTTGAAATTTCAATTTCATTCTTAATACGGGAGATTTCATTTGCATCTGTTTCATTATTTAAACCAGTAATTTTCTTTTTATATTCTTTGATTTGTTGTTCATAA", "aa": "MRNVKSLIIPELIFLPRKYMSPVQRVFSFKSFNLFKHLIKVDLPHPDLPIKAVILLRLMSRLISNNTCLFLLYQKLKFRISNTFFSSKSFLMTLSLVFLIFIRRFEISISFLIREISFASVSLFKPVIFFLYSLICCS", "aa_hexdigest": "7a406b4e37c3f148b83d888d25df9584", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16295.696799999985, "isoelectric_point": 10.490440559387206 }, "id": "JPCNHPFPBM_931", "locus": "GCA000292505_00931" }, { "type": "cds", "sequence": "contig_1", "start": 512513, "stop": 513142, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTAAAAATGTTTTTAACTTTGTAATTGATTCACTAAGTTCAATGCCATCTATTATTTATGGATTATTTGGACTTTCTTTCTTTTTAAGAGTCCTGCAGTTAAGTGCTGGAGGAGCTAATGGTACTAGTTTAATAGCAGGCATTCTAACTATTAGTGTTGTTATATTACCCTTCCTTATAAGAACTTGTCAACAAGCACTAAATAATGTCAGTTGGGATTTAAGAATTAGTGCTTTTGCTTTAGGTATAAGTAAACGTGAAGTTATTTTCAAAATAGTTTTACCTAGTGCTTTGAAAGGATTAATAGTTGCATTAATTTTGTCAATCAACAGAATTATTGCTGAAACTGCACCCTTCTTTATCACTTCAGGGTTATCATCTAGTAATTTATTTCATTTGTCATTGCCAGGTCAAACACTAACAACAAGGATATATGGACAGTTATTTTCTATTAATAGCAATGCAATAAGTGTTATGTTAGAAACATCATTGGTCTCTGTTGTTTTCTTAATTCTTTTAATCTTTTTCAGTTCTTATTTAATCCCGAGTTTATTTTTGTTAAATAAACAAAAATGGCTAGTAATTAAAAGTAAATTTCAGTCCTTTAAATTATGGAAAAGAACATAA", "aa": "MVKNVFNFVIDSLSSMPSIIYGLFGLSFFLRVLQLSAGGANGTSLIAGILTISVVILPFLIRTCQQALNNVSWDLRISAFALGISKREVIFKIVLPSALKGLIVALILSINRIIAETAPFFITSGLSSSNLFHLSLPGQTLTTRIYGQLFSINSNAISVMLETSLVSVVFLILLIFFSSYLIPSLFLLNKQKWLVIKSKFQSFKLWKRT", "aa_hexdigest": "cd4135b6a7b96c29c5415c540ae08a62", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23097.37010000001, "isoelectric_point": 10.48998928070068 }, "id": "JPCNHPFPBM_932", "locus": "GCA000292505_00932" }, { "type": "cds", "sequence": "contig_1", "start": 513167, "stop": 513343, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAGCAATTTTGCTGTTTGTATTTATTAATGGAAGTGTTGCTATTAATAATAATGGTTCTACTGTTTTTTCATTTGAAGCTGATTCAACTGGCAGAGCACTAGTAAATACTCTAGTAATTATTTTGATTACTATCACCATTACTTTTCCACTAGCACTTTTAATTGCAATTTGA", "aa": "MLAILLFVFINGSVAINNNGSTVFSFEADSTGRALVNTLVIILITITITFPLALLIAI", "aa_hexdigest": "4b00b38fded486895dad3aca19e75e35", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6151.298899999996, "isoelectric_point": 4.369065666198731 }, "id": "JPCNHPFPBM_933", "locus": "GCA000292505_00933" }, { "type": "cds", "sequence": "contig_1", "start": 513617, "stop": 514267, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAAGTTTTATTGTATCAATAGGAGCTTTAATTATTGCTAGTTATATAGGGGTTAGAACTTCATTTTTCCTTGTTTATCGATGCAAACCAAAAATAAGAAAAAAACTTTCACTTATTATTGATATCCTTTCAGGAATACCATCTGTAATTTTTGGATTATTTGCATCACAAATATTAAGCATTTTCTTTCGGGATATCTTGAAATTACCGCCGCTTTCACTTTTAAATGTGATAGCTATGCTTTCTTTTATGATCATTCCTATTGTTATTTCATTAACAACAAATACATTAACTTATGTAAATAACGATCTAATTAGTGTTGTTGTTTCCTTAGGGGAAAATAAAACAAGTGCGATCTACAAAATTATTAAAAAAGAAATTAAACCACAATTAACAGTTATTTTGACCTTAGCCTTTGCGAGAGCAATTAGTGAAACAATGGCTGTTAACTTTGTTTTGCAGAGTGTTAACTATCAAGAGGTAATTAACAACAATCGTTTTTTTACTTCTGATTTAAAAACACTGGGATCAGTTATTTCCACTTTTATTTTTTCAGAAAATGGAGATGAACAGATTAATGGTGTTTTATATATCTTTGGAATCATAATTTTGATATTAGTTTCATTGTTAAATTTCTTTGCCATTTGA", "aa": "MVSFIVSIGALIIASYIGVRTSFFLVYRCKPKIRKKLSLIIDILSGIPSVIFGLFASQILSIFFRDILKLPPLSLLNVIAMLSFMIIPIVISLTTNTLTYVNNDLISVVVSLGENKTSAIYKIIKKEIKPQLTVILTLAFARAISETMAVNFVLQSVNYQEVINNNRFFTSDLKTLGSVISTFIFSENGDEQINGVLYIFGIIILILVSLLNFFAI", "aa_hexdigest": "fc853dcdcdd492bec880a4c58a73b54a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24009.50100000001, "isoelectric_point": 9.518257331848144 }, "id": "JPCNHPFPBM_934", "locus": "GCA000292505_00934" }, { "type": "cds", "sequence": "contig_1", "start": 514277, "stop": 514477, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAAAGAAGATTAAAAAACGTTTAAAAAAAGAGAATCTTTTAAGAATCTTTTCAAAGACTTTAGCATTCTTGTTTTTAGTTTTATTTATAAGTTTTTTTGTTTTTCTTTTAACAGAAGCAACAAAAATTGGACCTGATTTTGCAAAGTCCTTGTTTAATCTTGAATTTAATTTAGGTAATAAACAGGCAGGAATTTGA", "aa": "MQKKIKKRLKKENLLRIFSKTLAFLFLVLFISFFVFLLTEATKIGPDFAKSLFNLEFNLGNKQAGI", "aa_hexdigest": "7bb897ee20a5264b8e15e2030168972f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7669.207399999995, "isoelectric_point": 10.358860588073728 }, "id": "JPCNHPFPBM_935", "locus": "GCA000292505_00935" }, { "type": "cds", "sequence": "contig_1", "start": 514735, "stop": 515121, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAATGAAAAAGATTACCCTTTAACAAGTTTTCCAAGAACTGGTGGGTCATTTGCTTCAGGAACAGCAGAAGCTTTTTTAAATTTTTCCGCCTTAAAATCAGATAAAACTTTAGATTCTCAAACTAGAGATATTTTGAAAGGAATTATTAATTATGGACCTCTTGCTAAACCTACTAGTGAAACAAATATTGAAGCTTTTAATACTTTTGTAACTAACTTACAAGATCCTAATCTTTTCGGGATGATTTACCTTAGTTTAGGATTTATTCAAAACAACCTTCAAGCTATTAAAAATAATGGTTTTGAGATACTACCAATTAAATATGAGAATAAGGAAGTTTTGCCTTCTAATCAAACTGTTTCCCAAAATGAGTATAAATGA", "aa": "MSNEKDYPLTSFPRTGGSFASGTAEAFLNFSALKSDKTLDSQTRDILKGIINYGPLAKPTSETNIEAFNTFVTNLQDPNLFGMIYLSLGFIQNNLQAIKNNGFEILPIKYENKEVLPSNQTVSQNEYK", "aa_hexdigest": "de5df9895df02f71adb9041ae27c8b70", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14250.831799999985, "isoelectric_point": 5.070288276672362 }, "id": "JPCNHPFPBM_936", "locus": "GCA000292505_00936" }, { "type": "cds", "sequence": "contig_1", "start": 515305, "stop": 515610, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTAAAGTTTAGAAATTTCTTTAAATTGACGCTACTAACATTAGCATCTGCTTTTTTTCTTAGTGGTTGTGCTAACATAAACCTGATTAGTGCTGTTGGTTCTTCTTCAGTTCAACCTTTACTAAATAAATTAAGTTCTTATTATGTTCTAAATGAAAATAATGACAATGATAAGCTTGTTGAAATTAGTGTGCAAGCAGGCGGATCAAATGCTGGAATCAGAGCAATCATTAACGGCTTTGCTGATATCGGAAATGTCTCTAAAAACCCTAAGGAATATGCAAAAGAGAATGAAAAAAAATGA", "aa": "MLKFRNFFKLTLLTLASAFFLSGCANINLISAVGSSSVQPLLNKLSSYYVLNENNDNDKLVEISVQAGGSNAGIRAIINGFADIGNVSKNPKEYAKENEKK", "aa_hexdigest": "7a1afa657b5932eb86f633012480eda9", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10959.39219999999, "isoelectric_point": 9.131189155578614 }, "id": "JPCNHPFPBM_937", "locus": "GCA000292505_00937" }, { "type": "cds", "sequence": "contig_1", "start": 516013, "stop": 516756, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTTCATTATCAAAAATAGTTTTAAATCTAAGGATAAGAATATTGATTTAGATATCAACAGTAACTCTTTAACATTTGTGGATAACCCTAAAAAATATTTCAAACATCTAGAGCCTACTAATGAAAAGAAAGGTATATTTTATGTTTTAGCAGAAATAAATAATAGCAATGAACTATTCAAATATGGTTCTGAAAGTGATTCTGAAATTATTAAAGACAAAATGTATTTTCTATCACAAAATCAAAAAAACCCTAAATTAAGAACATATTTATTTAAATTTCATACTAACAAACTTTTTGTTGATAAAAATGACCTGGGATTTAAGTTAGAAAAAGACAAGGTATTTTTATCAGTTGATAACTTAAAAATTGCTGAATTAGATTTAAAGTCATCTTCATTTAAATTTCTAGAGGATTATCAACTTGATTTTCATGAACCTTTTAGTTTAAATGACGAACAATTACTAGTTGATAAACTCAATATTACTCTAAGTGAAAAAAGATTGCAAACAACTAAAAACGTCCGCTTTAACCTAAAGAATAAATTTATTAACATCCATTTAGTAGAAAATAAAAATCAATTTAATTTAGTTTTTGATGTCGATGTTAGATCAAAAAAATTATTTATTAAAGGTGTTAATAATGATAACCAAGTATTTAGTATTAGCTATGATTTAAAAATAACAAATAATCAAACACTTTTAATTGTAGATGCCAATGGTTTTGATAATTCTATTTGA", "aa": "MAFIIKNSFKSKDKNIDLDINSNSLTFVDNPKKYFKHLEPTNEKKGIFYVLAEINNSNELFKYGSESDSEIIKDKMYFLSQNQKNPKLRTYLFKFHTNKLFVDKNDLGFKLEKDKVFLSVDNLKIAELDLKSSSFKFLEDYQLDFHEPFSLNDEQLLVDKLNITLSEKRLQTTKNVRFNLKNKFINIHLVENKNQFNLVFDVDVRSKKLFIKGVNNDNQVFSISYDLKITNNQTLLIVDANGFDNSI", "aa_hexdigest": "d93d3864bc9fca0b3ae220163c7b82e4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 28982.705900000044, "isoelectric_point": 8.726843452453611 }, "id": "JPCNHPFPBM_938", "locus": "GCA000292505_00938" }, { "type": "cds", "sequence": "contig_1", "start": 516862, "stop": 517500, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATTTGATATTAATTCGCTTGTTAGTCAAACTGAAAAAACTCTAAATAAAGAAACAATAGGTACAAAAATTAAACCAACTATTAAATTTAAAGATAAATTCATAAATGCAATTAAAGAAGTTAGTTTAATTAATCAAAGAATTGATGAATCAATTGATAAAAATGAAGCGTTAAAAAGCTTTAATATTTCAACCAATAATCAAAGTAATTTTTATCCAAATTTAGATTATTTATACAACCTATTGCAAATGTCACCAAACAATAAAGAAGAACTATTCTTTATTAGAAATTTACCAAGAATGGTCAAAACCATTTTTGATAGATCAACATTAACTGTTAAAGTAAAAATTGGTAATTCTGTAAATGAAATAACATTGTTAAGAAATAACAAGAATTATTTTGATTTAAGTTCAATTGAGGAGTTTTTAACAGAAAAACAGAAAGCAGATAATGAAATGAATATTGAATTTTTAGCACTTAATTTTTTTGTAGATGGATATGAATCAGAAAATATTAGTAACTATAGTGTTGAACCACTGTTTGACAGTATTAAAAAACTTTCAACTATCAAAAGAACTAAGGATGGTTTTGAATACAAATTTAAATATAGAAAAGATTTTAATGAACAGCGTTGA", "aa": "MEFDINSLVSQTEKTLNKETIGTKIKPTIKFKDKFINAIKEVSLINQRIDESIDKNEALKSFNISTNNQSNFYPNLDYLYNLLQMSPNNKEELFFIRNLPRMVKTIFDRSTLTVKVKIGNSVNEITLLRNNKNYFDLSSIEEFLTEKQKADNEMNIEFLALNFFVDGYESENISNYSVEPLFDSIKKLSTIKRTKDGFEYKFKYRKDFNEQR", "aa_hexdigest": "9df99f3453272cb1153c648f073701fe", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25017.10970000002, "isoelectric_point": 7.727454185485839 }, "id": "JPCNHPFPBM_939", "locus": "GCA000292505_00939" }, { "type": "cds", "sequence": "contig_1", "start": 517510, "stop": 517803, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTATAAATGAGTTTAAAGAGAAAATGAAAAAGGATAGAAAAATCATTGAAGCATTTACTAATAATCGTGAAATAACTCAAACGATATTAAGACTAGGTAATGGATTAGTAGTTAGACCAGAAACTGAAGATAGTAGACGTGAAGCATTCAACAGTAAATGGGATATTTTTAAACATACACCTATATTACCTGAACAAGAAGTTGTTCATCATCAAATAAGTTTTTATCTAAGGCAAAATAATCCTGATGAAGTTTTACCAATTAGTTTCAGTAATCTTTCAAAGCTTTGA", "aa": "MSINEFKEKMKKDRKIIEAFTNNREITQTILRLGNGLVVRPETEDSRREAFNSKWDIFKHTPILPEQEVVHHQISFYLRQNNPDEVLPISFSNLSKL", "aa_hexdigest": "877648ccd427e491af86a706198662e1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11495.98579999999, "isoelectric_point": 8.070232963562013 }, "id": "JPCNHPFPBM_940", "locus": "GCA000292505_00940" }, { "type": "cds", "sequence": "contig_1", "start": 518590, "stop": 518841, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTTCATATCTTTTTATTCCTTTATTAGTTTCTTTTCTTTTTCCCGTTGCTTTAGCTAATGCTTCACTTGAAAAAAACTTGGTAGATACTAATCTTTATCTCTCTCCAAACACAATTCAAAACAGAAGCTATACAGCTGGATTTAATTTAAAAAACGATTATTTTCGAAAAAGCTTTAATTTTGATAATGAAGTAATTCAAAGACCTAATCAAAATAATTATTTTGCAAAAACTTATTCGAATGAATGA", "aa": "MRSYLFIPLLVSFLFPVALANASLEKNLVDTNLYLSPNTIQNRSYTAGFNLKNDYFRKSFNFDNEVIQRPNQNNYFAKTYSNE", "aa_hexdigest": "e0c9180b16a3e8b9dd28cf214476f950", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9725.829599999994, "isoelectric_point": 9.047960472106933 }, "id": "JPCNHPFPBM_941", "locus": "GCA000292505_00941" }, { "type": "cds", "sequence": "contig_1", "start": 518954, "stop": 519448, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002EE4522", "UniRef:UniRef90_UPI0002EE4522" ], "nt": "ATGAATCTTTTAATAGATAAAAAGAAATCTAGCTTAGATCTTTTTCCTAAAGAGATCAAATTAACTACTCTAAAGATAAATAGTTCGCTTCATTTTTATTTAAATGTTGATGCAATTAAAAATCAGCTCAATATTGAAGTTAATATAAGTAAAAATAGATTAACTAGCCTTGTTTATGATATTGCTATCAAAAATGAAAATGAATTGCAAATTAGAACAACAAATAACTACTTAAATAAATATATTTGGTTTGATCTTGATAAGAAAAATAATCAGAAACTGAAAAATGAGCTTAAGTTATTTCTCTCATTAAAAAAATTTCAATTTAAAAAAGAACCTAATTTTAGCTTAAAGAAAAACAGCTATAGTTTTCAAATAGACAAAATTATTCAATCTAATAGTGAAGATAAAAAAACAGATATTATAGTATATTTGATTATTGGTTTTAGTGTTTTAGTTTTGTTTATCACTGTTTTTATTTACTTTCATAAGTGA", "aa": "MNLLIDKKKSSLDLFPKEIKLTTLKINSSLHFYLNVDAIKNQLNIEVNISKNRLTSLVYDIAIKNENELQIRTTNNYLNKYIWFDLDKKNNQKLKNELKLFLSLKKFQFKKEPNFSLKKNSYSFQIDKIIQSNSEDKKTDIIVYLIIGFSVLVLFITVFIYFHK", "aa_hexdigest": "2f74aa835235b30e1b57e012b9b89a87", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0002EE4522", "query_cov": 1.0, "subject_cov": 0.9162011173184358, "identity": 1.0, "score": 303.0, "evalue": 7.19e-104, "valid": true, "uniref50_id": "UniRef50_UPI0002EE4522" }, "pscc": { "uniref50_id": "UniRef50_UPI0002EE4522", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002EE4522" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19476.589199999995, "isoelectric_point": 9.657186698913574 }, "id": "JPCNHPFPBM_942", "locus": "GCA000292505_00942" }, { "type": "cds", "sequence": "contig_1", "start": 519626, "stop": 520126, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTTCTATTCAGAAAACTAAAAATGGTTTTCAATATAGTTTAAAGTTTAGATCTGAATACAATGAACATCATATTTTAAAAGATCATTTTTTAATTCCTATTGTTACAAATCAGAAAAATTTCAGTGTTAATGACATTGAAAAAAACGGATTAAATAGTTATCAAAGAGAACAAATTACATATGCAATTAAAAATAGTTTTACTTCTCAAAAAGAAAACAATTTAAATATTTCATCCGCTACAATAAAATACATAAAAGATCCTGAGAAATTAATTAAAAAATCTTTAATTAAGCCCAGTGTTAAAAATGGTATATTCTATGTTTCAGCACAAATAATTAACAGTAATGATTTAACTAAATGGGGTTCTAAAAATGATAGCGAAATCATTAAAGATAAGATGTATTTCTTAGAACAAAATAAAAACTTTCCTGCAATAAGAACTTATCTTTTTCAAATGAGAACCAAAAAGTTAGTTTTAAATGTTAATGATATTTGA", "aa": "MPSIQKTKNGFQYSLKFRSEYNEHHILKDHFLIPIVTNQKNFSVNDIEKNGLNSYQREQITYAIKNSFTSQKENNLNISSATIKYIKDPEKLIKKSLIKPSVKNGIFYVSAQIINSNDLTKWGSKNDSEIIKDKMYFLEQNKNFPAIRTYLFQMRTKKLVLNVNDI", "aa_hexdigest": "1789ac8c6b497b4f70dc97ab62b91294", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19459.105399999997, "isoelectric_point": 9.697866249084473 }, "id": "JPCNHPFPBM_943", "locus": "GCA000292505_00943" }, { "type": "cds", "sequence": "contig_1", "start": 520175, "stop": 520819, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002F2F2D0", "UniRef:UniRef90_UPI0002F2F2D0" ], "nt": "TTGATTGATACTAATCTTTATCTAGTTAAAGATGATTTTCAATCACAAAATCAATTAACTATAGCAACAAATCAACTTGCTAAGATCATTGTTAACCAAATTGAATTTGATTCAAATTCTTTAATAGCTAATCCAACTACTGTTTTAAACAAAGAACTAATAGGATCAAAAATAACACCAAAGCTGAAATTTAGTGATCAATTTTCTAATGCAATTGAAATGGTAAGTAAGCTAAATCAAGAGTTTGATCAATTAGCAAATAAAGATAAAACTTTTTTTCAATTTGCTTTAGATTTATTAGAAAAACAGGAAGAATCTAAATTTGATTTTGAACCAAAAGATGAAAGAATTGATGCAATATTTTTCTTAAGTAATTTAATTAACATTAATCCAAAACAAGAGAAAACATTAAATTTTATTCGGATATTACCCAACCTAATTAAAAGTATTTTTAAAGATACTACCATCACGATAAATATAAAGATAGGTGGCAAAAATAAAGTAATTACTTTTATAGAAAATGGCAGTAACGTTTTTTTATTAAGTGATGTTGAAAATTTCTTAAATGCTGATCAAACTGGTATTAATTTTTATGAAATTGAGTTTTTAACTTTTGATTTTATTGTTGTGAATAAAACAGGATGA", "aa": "MIDTNLYLVKDDFQSQNQLTIATNQLAKIIVNQIEFDSNSLIANPTTVLNKELIGSKITPKLKFSDQFSNAIEMVSKLNQEFDQLANKDKTFFQFALDLLEKQEESKFDFEPKDERIDAIFFLSNLININPKQEKTLNFIRILPNLIKSIFKDTTITINIKIGGKNKVITFIENGSNVFLLSDVENFLNADQTGINFYEIEFLTFDFIVVNKTG", "aa_hexdigest": "da89400994f088c781a1f598fc9493ea", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 519872, "stop": 520900, "observations": { "mutation": [ 520177, 520876, 520894 ], "stop": [ 520177, 520876, 520894 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0002F2F2D0", "query_cov": 1.0, "subject_cov": 0.4562899786780384, "identity": 0.995, "score": 407.0, "evalue": 8.9e-140, "gene_start": 1, "gene_end": 214, "reference_start": 28, "reference_end": 241, "reference_sequence": "MSWKRYLKWVSFAIIPLLFANTSIKSKLIDTNLYLVKDDFQSQNQLTIATNQLAKIIVNQIEFDSNSLIANPTTVLNKELIGSKITPKLKFSDQFSNAIEMVSKLNQEFDQLANKDKTFFQFALDLLEKQEESKFDFEPKDERIDAIFFLSNLININPKQEKTLNFIRILPNLIKSIFKDTTITINIKIGGKNKVITFIENGSNVFLLSDVENFLNADQTGINFYEIEFLTFDFIVVNKTGWTLKNQPVDSFFKSVKNLPSIQKTKNGFQYSLKFRSEYNEHHILKDHFLIPIVTNQKNFSVNDIEKNGLNSYQREQITYAIKNSFTSQKENNLNISSATIKYIKDPEKLIKKSLIKPSVKNGIFYVSAQIINSNDLTKWGSKNDSEIIKDKMYFLEQNKNFPAIRTYLFQMRTKKLVLNVNDIWFKSSGDKLRVIVNNVEIDEFNPKENNTSIFWKLWSSYKWLFFFS" }, "paralog": false, "identity": 0.9912280701754386, "score": 656.0, "evalue": 2.89e-240, "description": "Internal stop codon at 520177, 520876, 520894. Nonsense mutation around 520177, 520876, 520894.", "psc": { "uniref90_id": "UniRef90_UPI0002F2F2D0", "uniref50_id": "UniRef50_UPI0002F2F2D0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0002F2F2D0", "UniRef:UniRef50_UPI0002F2F2D0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0002F2F2D0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002F2F2D0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 24550.77500000003, "isoelectric_point": 4.768586921691894 }, "id": "JPCNHPFPBM_944", "locus": "GCA000292505_00944" }, { "type": "cds", "sequence": "contig_1", "start": 520956, "stop": 521354, "strand": "-", "frame": 2, "gene": "rpsI", "product": "30S ribosomal protein S9", "db_xrefs": [ "COG:COG0103", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022627", "KEGG:K02996", "RefSeq:WP_010869475.1", "SO:0001217", "UniParc:UPI00001351B7", "UniRef:UniRef100_P47656", "UniRef:UniRef50_P31782", "UniRef:UniRef90_P47656" ], "nt": "ATGGATAAAAAATCTTTTTATGGACTTGGTCGTCGTAAATCTTCTACTGCTAAAGTTTATTTATATCAAAGCAAAGATAAGGGTAAAATAACCATTAATCATCGTAATCCTAGCGATTATTTTCCAAATAAATTGGTGATTCAAGATATGGAACAACCCTTAGAGTTAACCAAACTTAAGGATAACTTTGATATCAATGTTGTTGTTAAAGGTGGAGGATTTACTGGGCAGGCAGGAGCCATTAGATTAGGTATTGTTCGTGCTTTAATAAAATTTAATCCAGATCTTAAAAAGTTATTAAAAACCAAAAAATTAACAACACGTGATAAGCGTGCTAAGGAACGCAAAAAATTTGGTTTATATGGTGCTAGACGTGCACCACAATTTACTAAGCGTTAG", "aa": "MDKKSFYGLGRRKSSTAKVYLYQSKDKGKITINHRNPSDYFPNKLVIQDMEQPLELTKLKDNFDINVVVKGGGFTGQAGAIRLGIVRALIKFNPDLKKLLKTKKLTTRDKRAKERKKFGLYGARRAPQFTKR", "aa_hexdigest": "094428f8d3565fd2dfcbc16c293a5f25", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00001351B7", "ncbi_nrp_id": "WP_010869475.1", "uniref100_id": "UniRef100_P47656", "db_xrefs": [ "SO:0001217", "UniParc:UPI00001351B7", "RefSeq:WP_010869475.1", "UniRef:UniRef100_P47656" ] }, "ips": { "uniref100_id": "UniRef100_P47656", "uniref90_id": "UniRef90_P47656" }, "psc": { "uniref90_id": "UniRef90_P47656", "gene": "rpsI", "product": "30S ribosomal protein S9", "uniref50_id": "UniRef50_P31782", "cog_id": "COG0103", "cog_category": "J", "kegg_orthology_id": "K02996", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P31782", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P31782" ], "product": "Small ribosomal subunit protein uS9" }, "genes": [ "rpsI" ], "id": "JPCNHPFPBM_945", "locus": "GCA000292505_00945" }, { "type": "cds", "sequence": "contig_1", "start": 521366, "stop": 521551, "strand": "-", "frame": 3, "gene": "rplM", "product": "50S ribosomal protein L13", "db_xrefs": [ "COG:COG0102", "COG:J", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0017148", "GO:0022625", "KEGG:K02871", "SO:0001217", "UniRef:UniRef50_P47657", "UniRef:UniRef90_P47657" ], "nt": "ATGGGTGGAATTAAAAAAACTAGTGGAAGGGATATGATAAACAAAAATTCAGATAAACTTGTTTTCAATGCTGTTAAGGGAATGTTACCTGATAATCGTTTAAGCAGAAGATTAATAACTAAAGTACATGTTTTTAAGAATGATAAGCACAACATGGAAGCACAAAAACCAACATTACTTAATTGA", "aa": "MGGIKKTSGRDMINKNSDKLVFNAVKGMLPDNRLSRRLITKVHVFKNDKHNMEAQKPTLLN", "aa_hexdigest": "d8458e5909285375f3e86844599ca9a5", "start_type": "ATG", "rbs_motif": null, "genes": [ "rplM" ], "truncated": "3-prime", "pseudogene": { "start": 521366, "stop": 521800, "observations": { "mutation": [ 521572 ], "stop": [ 521572 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47657", "query_cov": 1.0, "subject_cov": 0.4178082191780822, "identity": 1.0, "score": 122.0, "evalue": 1.36e-34, "gene_start": 1, "gene_end": 61, "reference_start": 84, "reference_end": 144, "reference_sequence": "MQKTSMLTKEEAIKNRKWYLVDASGLVLGKLAVKAANLIRGKNKANFTPNQDCGDHLIIINSDQVVLTGNKKDNEFWYHHSQYMGGIKKTSGRDMINKNSDKLVFNAVKGMLPDNRLSRRLITKVHVFKNDKHNMEAQKPTLLNWS" }, "paralog": false, "identity": 0.9930555555555556, "score": 289.0, "evalue": 1.09e-102, "description": "Internal stop codon at 521572. Nonsense mutation around 521572.", "psc": { "uniref90_id": "UniRef90_P47657", "gene": "rplM", "product": "50S ribosomal protein L13", "uniref50_id": "UniRef50_P47657", "cog_id": "COG0102", "cog_category": "J", "kegg_orthology_id": "K02871", "go_ids": [ "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0017148", "GO:0022625" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47657", "UniRef:UniRef50_P47657", "COG:COG0102", "COG:J", "KEGG:K02871", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0017148", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47657", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47657" ], "product": "Large ribosomal subunit protein uL13" } }, "id": "JPCNHPFPBM_946", "locus": "GCA000292505_00946" }, { "type": "cds", "sequence": "contig_1", "start": 521570, "stop": 521800, "strand": "-", "frame": 3, "gene": null, "product": "50S ribosomal protein L13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0025A24A7C" ], "nt": "ATGCAAAAAACATCGATGCTTACAAAGGAAGAAGCCATTAAAAACAGGAAGTGGTATCTTGTTGACGCTAGTGGTTTGGTTTTAGGCAAATTAGCAGTTAAAGCTGCAAATTTAATTAGAGGAAAAAATAAAGCTAATTTTACTCCTAATCAAGATTGTGGAGATCATCTAATAATTATTAACAGTGATCAAGTGGTTTTAACTGGAAATAAAAAAGACAATGAGTTTTGA", "aa": "MQKTSMLTKEEAIKNRKWYLVDASGLVLGKLAVKAANLIRGKNKANFTPNQDCGDHLIIINSDQVVLTGNKKDNEF", "aa_hexdigest": "cca961f7519060653a5ca6c9aeb7432b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0025A24A7C", "query_cov": 0.9342105263157895, "subject_cov": 0.8658536585365854, "identity": 0.535, "score": 84.7, "evalue": 4.78e-20, "valid": false, "gene": "rplM", "product": "50S ribosomal protein L13", "uniref50_id": "UniRef50_UPI0025A24A7C", "kegg_orthology_id": "K02871", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0025A24A7C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0025A24A7C" ], "product": "50S ribosomal protein L13" }, "genes": [], "id": "JPCNHPFPBM_947", "locus": "GCA000292505_00947" }, { "type": "cds", "sequence": "contig_1", "start": 521838, "stop": 521954, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGAACTTAACCCAATTGGAAAACTTAGCTAAGCCTTTTTTAAAAGAAAAGAAACGCTCTTTATCACAAAAAATGGTGGATAAATACTTTAAAGGACTTTTTGAAGAAAAGTAA", "aa": "MLNLTQLENLAKPFLKEKKRSLSQKMVDKYFKGLFEEK", "aa_hexdigest": "c693fa80aa9868ed8366ba16f4a65cf7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4574.410099999999, "isoelectric_point": 9.698317527770996 }, "id": "JPCNHPFPBM_948", "locus": "GCA000292505_00948" }, { "type": "cds", "sequence": "contig_1", "start": 522297, "stop": 523403, "strand": "-", "frame": 2, "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "db_xrefs": [ "COG:COG2812", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0006261", "GO:0009360", "GO:0016887", "GO:0046872", "KEGG:K02343", "SO:0001217", "UniRef:UniRef50_P47658", "UniRef:UniRef90_P47658" ], "nt": "GTGTGTAAAAGTATTAACACTAATAGTGCCATTGATATAGTTGAGATAGATGCAGCTTCTAAAAATGGTATTAATGATATTAGAGAGTTGGTAGAAAATGTTTTCAATCATCCCTTCACATTTAAAAAAAAGGTTTATATTTTAGATGAAGCACACATGTTAACCACCCAATCATGGGGTGGCTTGTTAAAAACTTTAGAAGAATCACCACCTTATGTTCTTTTTATTTTTACAACTACTGAATTTAACAAGATTCCATTAACAATTTTGTCCAGATGTCAAAGCTTCTTTTTTAAAAAAATAACTAGTGATTTAATCCTTGAAAGATTAAATGATATAGCAAAAAAAGAAAAGATTAAGATAGAGAAAGATGCATTAATAAAAATTGCTGATTTATCCCAAGGTTCATTGCGTGATGGGCTTAGCTTACTAGATCAAATTAGCAATTTCTCTGATAGTGAAAAAATTAGTATTACTGATGTTGAAAAAACATTTAATATCGTTGATAGAAATGCAAAATTTACTTTTATTAAAGCAGTTTTATCAGGAGATATAAAAGAAGCATTTAACTTGCTTGATGATTTTGAAAGTAATGGTCTAAATTTCACTTACTTCTTAAGAGAGTTGTTTGCGCTAACAGTTAATTTATATGCGTATGCAAAACTAAAAAATATTAATGTTTTAGATTCAACTGAAAAAACAATGATAGAAACACTTAATTTTGAAAAGCAGCATTATGCGTTTTTAATTAAGGCCATTGAAGAAAATACTAATTTTGGTTTAAGTCAATTAACTTTAATTGATAGGCTAAAAGCTATTGTTATTAGCTATAATGAATTTTTTAATCAAAAACCTTTAACAATAAGCTCACCAAGTAATGAAAAATCTCTACACTTAGAAACTGAATATTTGGAAAAGAAAAAAATAAAAAAATCAAATCACAAACAAGACCAAAAGCACTTTTCTTTATTTGAAAAATCTTTTATAGATAAATCAGAAAAGACACCTAAAAATGATGAAGTTACTAATAACAAATTCTTAGATACAAGCAAACTAAATCTTGCCAATATTGCTCTTGCAATTAATGCATTTAATGACAATAAATGA", "aa": "MCKSINTNSAIDIVEIDAASKNGINDIRELVENVFNHPFTFKKKVYILDEAHMLTTQSWGGLLKTLEESPPYVLFIFTTTEFNKIPLTILSRCQSFFFKKITSDLILERLNDIAKKEKIKIEKDALIKIADLSQGSLRDGLSLLDQISNFSDSEKISITDVEKTFNIVDRNAKFTFIKAVLSGDIKEAFNLLDDFESNGLNFTYFLRELFALTVNLYAYAKLKNINVLDSTEKTMIETLNFEKQHYAFLIKAIEENTNFGLSQLTLIDRLKAIVISYNEFFNQKPLTISSPSNEKSLHLETEYLEKKKIKKSNHKQDQKHFSLFEKSFIDKSEKTPKNDEVTNNKFLDTSKLNLANIALAINAFNDNK", "aa_hexdigest": "ea6d560d97375f74a2b53a47aee34959", "start_type": "GTG", "rbs_motif": null, "genes": [ "dnaX" ], "truncated": "both", "pseudogene": { "start": 521994, "stop": 523631, "observations": { "mutation": [ 522098, 522299, 523436 ], "stop": [ 522098, 522299, 523436 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47658", "query_cov": 1.0, "subject_cov": 0.6164154103852596, "identity": 0.997, "score": 697.0, "evalue": 1.4e-249, "gene_start": 1, "gene_end": 368, "reference_start": 77, "reference_end": 444, "reference_sequence": "MHQVFYQKYRPINFKQTLGQESIRKILVNAINRDKLPNGYIFSGERGTGKTTFAKIIAKAINCLNWDQIDVCNSCDVCKSINTNSAIDIVEIDAASKNGINDIRELVENVFNHPFTFKKKVYILDEAHMLTTQSWGGLLKTLEESPPYVLFIFTTTEFNKIPLTILSRCQSFFFKKITSDLILERLNDIAKKEKIKIEKDALIKIADLSQGSLRDGLSLLDQISNFSDSEKISITDVEKTFNIVDRNAKFTFIKAVLSGDIKEAFNLLDDFESNGLNFTYFLRELFALTVNLYAYAKLKNINVLDSTEKTMIETLNFEKQHYAFLIKAIEENTNFGLSQLTLIDRLKAIVISYNEFFNQKPLTISSPSNEKSLHLETEYLEKKKIKKSNHKQDQKHFSLFEKSFIDKSEKTPKNDEVTNNKFLDTSKLNLANIALAINAFNDNKWINHFQNLLSVFQTKFNDKDKQNNLSYFNNFIDKYSARDIVKATKIVKASSFGIVILFEDQKIAMRLWKEAIEEGNVQATIFQIFNQNLFLASFSEHQYKTTITEETKNQKYQTEVLNLTQLENLAKPFLKEKKRSLSQKMVDKYFKGLFEEK" }, "paralog": false, "identity": 0.9926605504587156, "score": 1056.0, "evalue": 0.0, "description": "Internal stop codon at 522098, 522299, 523436. Nonsense mutation around 522098, 522299, 523436.", "psc": { "uniref90_id": "UniRef90_P47658", "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_P47658", "cog_id": "COG2812", "cog_category": "L", "kegg_orthology_id": "K02343", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0006261", "GO:0009360", "GO:0016887", "GO:0046872" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47658", "EC:2.7.7.7", "UniRef:UniRef50_P47658", "COG:COG2812", "COG:L", "KEGG:K02343", "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0006261", "GO:0009360", "GO:0016887", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_P47658", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47658" ], "product": "DNA polymerase III subunit gamma/tau" } }, "id": "JPCNHPFPBM_949", "locus": "GCA000292505_00949" }, { "type": "cds", "sequence": "contig_1", "start": 523434, "stop": 523631, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00004.35", "PFAM:PF13177.12" ], "nt": "ATGCACCAAGTTTTTTATCAAAAATATCGGCCAATCAATTTCAAACAAACCCTAGGACAAGAATCGATAAGAAAAATCTTGGTGAATGCTATTAACAGGGATAAACTACCTAATGGTTATATCTTTTCAGGTGAAAGAGGAACAGGTAAAACTACTTTTGCAAAGATAATAGCAAAAGCGATAAACTGCTTAAATTGA", "aa": "MHQVFYQKYRPINFKQTLGQESIRKILVNAINRDKLPNGYIFSGERGTGKTTFAKIIAKAINCLN", "aa_hexdigest": "d3470f6a56b9b116dda894a5ae7cc9fc", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028A06DEE", "query_cov": 0.9538461538461539, "subject_cov": 0.96875, "identity": 0.5, "score": 69.3, "evalue": 2.39e-14, "valid": false, "product": "AAA family ATPase", "uniref50_id": "UniRef50_A0A7V9ZZE4" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00004.35", "name": "AAA", "length": 22, "aa_cov": 0.3384615384615385, "hmm_cov": 1.0, "evalue": 7.400641672177023e-06, "score": 21.570632934570312, "start": 41, "stop": 62 }, { "id": "PF13177.12", "name": "DNA_pol3_delta2", "length": 46, "aa_cov": 0.7076923076923077, "hmm_cov": 1.0, "evalue": 1.785940081679805e-09, "score": 32.901039123535156, "start": 19, "stop": 64 } ], "seq_stats": { "molecular_weight": 7458.626899999999, "isoelectric_point": 10.176801872253417 }, "id": "JPCNHPFPBM_950", "locus": "GCA000292505_00950" }, { "type": "cds", "sequence": "contig_1", "start": 523637, "stop": 525271, "strand": "-", "frame": 3, "gene": null, "product": "UvrABC system protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PGE6" ], "nt": "ATGTCTGAAATAACATGTAAAAAGTGTCATGGAAAAAAATTAATAAAAGACGCTTTAAGTGTTAAGTTAGGAGGAATTGACATTATTAGCTTTACTGAACTTTCCATTGATAAAAGTATTGATTTTCTATTAAAACTAGAGTTAAATGATGAGCAAAAGAAGATCGGTGAATTAGCTTTAAAAGAGATTATTAATCGTCTTTCTTTTCTTAAAAATGTTGGTTTAGATTATCTTAATCTTGCAAGAAGAGCTTCTACGCTGTCAGGTGGAGAAGCACAAAGAATTAGATTAGCTACCCAAATTGGTTCTCAACTTACTGGTGTTTTATATGTAATGGATGAACCTTCTATTGGATTGCATCAAAAAGACAATATGCGTTTAATTAAAACAATGATGGTAATGCGTGATTTAGGTAACACCTTATTAGTAGTTGAGCATGATAGTGAAACAATGTTAGCGGCAGATTATTTAATTGATATTGGTCCTAAAGCAGGTAATGAAGGTGGTGAATTAGTTGCTTGCGGTACACCTTTACAAGTAATGGAAAACTCAAACTCAATTACTGGACAATATCTTAGTGGTAAAAAACAAATCTCCATTCCAAAAAATAGACATAGTGGTAATGGTAAAACAATTATTATCAAGGGTGCTAAAGTTAATAATTTGAAAAATATTAATGTTACGATTCCTTTAAATAAATTGGTTTTGATAACAGGAGTTTCAGGTTCTGGAAAATCCTCTTTAATTAATCAAACATTAGTTCCAGCTTTAGAAAGAATTCTTTATCGTAAAGGTGTTAAAAAAGATACATATAAGGAAATAATTGGTGCTAACAACATTGATAAGATAATTGTTGTCTCTCAAGACCCAATTGGTAGAACACCACGTTCTAATCCTGCAACCTATATTAGTGTTTTTGATGATATTCGTGATTTATTTGCCAACACAAAAGAAGCTAAAGCAAGAGGATATACAAATTCACGATTTTCTTTTAATGTTCCAGGTGGTAGGTGTGATAAGTGTTTTGGTGATGGTGTGATTCGCATTGAAATGCATTTTTTACCTGATGTTTATGTCAAATGTGAAGTATGTAATGGCAAGAAGTACAATTCACAAACACTGGAAATTAAATATTTGGGAAAATCAATTTTTGATGTTTTACAAATGTCTTGTAAAGAAGCTTATGAATTTTTTAAAGCTATCCCAAATATATCACGTAAACTAAGGTTGTTATGTGATGTTGGTTTAGAATATTTGCAATTAGGTATTAATGTCACTTTTCTTTCAGGTGGGGAAGCACAGAGAATTAAGTTATCTAAGTTTTTACAAAAAAAATCTACTGGTAAAACTTTGTTTGTTTTAGATGAACCCTCTACTGGCTTACATTTAGAAGATATAAACAAACTATTAACAATAATTCAAAGAATCATTAAGAATGGTGATACAGTAGTTGTTATAGAACATAACTTAGATATTATTAAGGTTGCTGACTATATCATTGATTTAGGTCCTGAAGGTGGTGACAATGGTGGTCAAATTGTTGCTCAAGGAACACCTGAACAACTTATAAACCAAGTTAATAAATCTTATACTGCCCAATATTTGTCCAAAATTTTAAAACCAGATTCAATTTAA", "aa": "MSEITCKKCHGKKLIKDALSVKLGGIDIISFTELSIDKSIDFLLKLELNDEQKKIGELALKEIINRLSFLKNVGLDYLNLARRASTLSGGEAQRIRLATQIGSQLTGVLYVMDEPSIGLHQKDNMRLIKTMMVMRDLGNTLLVVEHDSETMLAADYLIDIGPKAGNEGGELVACGTPLQVMENSNSITGQYLSGKKQISIPKNRHSGNGKTIIIKGAKVNNLKNINVTIPLNKLVLITGVSGSGKSSLINQTLVPALERILYRKGVKKDTYKEIIGANNIDKIIVVSQDPIGRTPRSNPATYISVFDDIRDLFANTKEAKARGYTNSRFSFNVPGGRCDKCFGDGVIRIEMHFLPDVYVKCEVCNGKKYNSQTLEIKYLGKSIFDVLQMSCKEAYEFFKAIPNISRKLRLLCDVGLEYLQLGINVTFLSGGEAQRIKLSKFLQKKSTGKTLFVLDEPSTGLHLEDINKLLTIIQRIIKNGDTVVVIEHNLDIIKVADYIIDLGPEGGDNGGQIVAQGTPEQLINQVNKSYTAQYLSKILKPDSI", "aa_hexdigest": "7fc3ae9816ba064af80282cdda0ced0c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PGE6", "query_cov": 0.9301470588235294, "subject_cov": 0.9980276134122288, "identity": 0.6859999999999999, "score": 709.0, "evalue": 1.47e-252, "valid": false, "gene": "uvrA", "product": "excinuclease ABC subunit UvrA", "uniref50_id": "UniRef50_A0A3B0PGE6", "kegg_orthology_id": "K03701", "go_ids": [ "GO:0003677", "GO:0006289", "GO:0009380", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PGE6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PGE6" ], "product": "UvrABC system protein A" }, "genes": [], "id": "JPCNHPFPBM_951", "locus": "GCA000292505_00951" }, { "type": "cds", "sequence": "contig_1", "start": 525515, "stop": 526033, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17760.6" ], "nt": "GTGCAATTATTAGCACCTACTGTTAAAAATCAGCGCGGCATTTTTACAAATGAATTTATTAAATACAAGCAATTAGGTTTTCTTAGAGTCTTAGTTGATGGCCAGATTTACACCTTAGATGATGAAATTAAACTTGATAAAAATACTAAACACAACATTAGTGTAGTGATCGATAGAATTATCATCAATAAAGATAATCAAACTTATTCAAGGATAGTTGATAGCATTGAAACCATTGATAGGTTAACTAATGGCAAGATAGAAGTTCTTAAGGAAGATGGAACAATATTAAATTTCAGCAAAAATCATGGTTGTGATAAATGTGGTTTTTCTATTAGTGAATTGGAACCAAGATTATTTTCCTTTAACTCCCCTTTAGGTTCATGTTCATATTGCAAAGGACTTGGTTTTAGTTATGAACCTGATGTAGACAAGATAATTGCTGATTCTAAACTTTCTATTAACCAAGGAGCCATTGATATTTTTAAAAATATTGTGCATGGAACTTCTTTGGATTGA", "aa": "MQLLAPTVKNQRGIFTNEFIKYKQLGFLRVLVDGQIYTLDDEIKLDKNTKHNISVVIDRIIINKDNQTYSRIVDSIETIDRLTNGKIEVLKEDGTILNFSKNHGCDKCGFSISELEPRLFSFNSPLGSCSYCKGLGFSYEPDVDKIIADSKLSINQGAIDIFKNIVHGTSLD", "aa_hexdigest": "b8f3495ab2700b05fcf00e9b121af830", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17760.6", "name": "UvrA_inter", "length": 92, "aa_cov": 0.5348837209302325, "hmm_cov": 0.9782608695652174, "evalue": 6.726668585819907e-29, "score": 95.11275482177734, "start": 1, "stop": 92 } ], "seq_stats": { "molecular_weight": 19364.896699999994, "isoelectric_point": 5.9281023025512685 }, "id": "JPCNHPFPBM_952", "locus": "GCA000292505_00952" }, { "type": "cds", "sequence": "contig_1", "start": 526473, "stop": 526736, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTATTTAGTGATTTAAACAAAATTAACAAAAATGAAAGTCTAAAGCTTTACTTGCTATTTAAACTTTTGTTAAATCCTAAGTTACTAATGATAAACTGCTGCAATGATTTCACTAACCACGCTTATAATTTCATTCGTGGACTTTTGATCTCATATCAAAATAAACAAGGTATTGCGCTTATTTTCAATGATCCTAATAATAAGTTAGTTAAAAATTTTTCAATTAAAAACATTGACTTTGAAACCGGAATGAAAAAATAA", "aa": "MLFSDLNKINKNESLKLYLLFKLLLNPKLLMINCCNDFTNHAYNFIRGLLISYQNKQGIALIFNDPNNKLVKNFSIKNIDFETGMKK", "aa_hexdigest": "429b3469fa69df6b62b9c4719dd5368c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10172.93089999999, "isoelectric_point": 9.517290306091308 }, "id": "JPCNHPFPBM_953", "locus": "GCA000292505_00953" }, { "type": "cds", "sequence": "contig_1", "start": 526737, "stop": 527006, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGATATTGTTTTTTAAACAAAACATTACACTTAAGACTTAAAAAAACAAACAAGAAAATTAAGAGAACTTTTTTTATTGATTCAGAAGAGTGCAATATCAATCGTTTAGAAGCTTCAAATAAGCTTTATCTTAATGTTTTGAATTCACAATTAAAAACAATTAATTTCTTTTTAAGATCACAAAAAAATCTTAAAAAACTTAACTTTATTAACAACATTAATTTATTGATAAATCAAACCAAAAAGAATGGTATTAGCAGCTGA", "aa": "MKRYCFLNKTLHLRLKKTNKKIKRTFFIDSEECNINRLEASNKLYLNVLNSQLKTINFFLRSQKNLKKLNFINNINLLINQTKKNGISS", "aa_hexdigest": "c6435208cd0eac410aca869cb3e02f24", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10620.432999999986, "isoelectric_point": 10.466264915466308 }, "id": "JPCNHPFPBM_954", "locus": "GCA000292505_00954" }, { "type": "cds", "sequence": "contig_1", "start": 527457, "stop": 527810, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTGCACGTTTCATTCTAACGAAAAAAGAGGTTCATGAATTTTTGATCAAAGCACGTTTATCTTTTATTAAAAATTTTGTTAAACAATCAAACAATTTATATGTTCATGAAAAATTAGTTAATGAACTAAAAGAAAACATCTTATTAAATCAAAATCATTATAGAGGTAAGCATAATAACAGTTTTTCAGTTCTAATCCAAAAAACAGTTTTCAAACAGTTTATTGATACAGTGCAATATGCAATTGGTTTAATCAGCATGAGAAAGAGTTTGGAGACTTTTAACTTTGCATTTCAAATAAAGCAATATTTTTATGAAAGCTTATTGGCTAATTACCAAAAAATTCACTGA", "aa": "MSARFILTKKEVHEFLIKARLSFIKNFVKQSNNLYVHEKLVNELKENILLNQNHYRGKHNNSFSVLIQKTVFKQFIDTVQYAIGLISMRKSLETFNFAFQIKQYFYESLLANYQKIH", "aa_hexdigest": "84e364a5f454017233827f7d926ac2da", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14021.152999999988, "isoelectric_point": 9.859166145324707 }, "id": "JPCNHPFPBM_955", "locus": "GCA000292505_00955" }, { "type": "cds", "sequence": "contig_1", "start": 527844, "stop": 527996, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGAAATTAAGAAGATGGATTTACTTTCTCCAAAATTACTTGTAGAAACTATTGATGAAAGTATTAATTTAGTCAATAAAATCTTTGCTAATATTCAAAATCTTTATCAAGAAATTATCAGCTTAAAATCAGGTCCTAATTTGAACTGA", "aa": "MKEIKKMDLLSPKLLVETIDESINLVNKIFANIQNLYQEIISLKSGPNLN", "aa_hexdigest": "285690f0b19ed777462c4dd447e6441b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5714.691599999999, "isoelectric_point": 6.090263938903808 }, "id": "JPCNHPFPBM_956", "locus": "GCA000292505_00956" }, { "type": "cds", "sequence": "contig_1", "start": 528090, "stop": 528527, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAATTGAAAATGTTAATCAAAAAACTAGTAAAGTCATTAATCAGTTTTTTACATCAAAATTTATTAATTCAATCTACTTTGAACAAGTTGATTTTGAATACAATAATTTTTTAACTTTAATTGAAAAAAACGAAAGAGAACTTGTTGATTTTAATTGCATGTTTTTCAATCAATTATTTACTTTTAATAACAAAGTAATAAAAAGTTTTTTTGAGCGTTACTATGCATTGAACGAACAAAAAAATAACTTAAAAAACAAACTGAAAATAGCTGCTTATGAAGCTAAATCACGAGATAACAGTTATTTTGAAAAATTGCAAATTGAAAGAGCTAAACAAAGACTTTTTGATGCTAAGAAAACATTCTCAAAACAACATAAAAATGCTTTGCAATTAATTAATTCATTTACCTTTTCTTTAATTTTTAGTGGTTGA", "aa": "MKIENVNQKTSKVINQFFTSKFINSIYFEQVDFEYNNFLTLIEKNERELVDFNCMFFNQLFTFNNKVIKSFFERYYALNEQKNNLKNKLKIAAYEAKSRDNSYFEKLQIERAKQRLFDAKKTFSKQHKNALQLINSFTFSLIFSG", "aa_hexdigest": "c9c279d5abd121adf29633adf5310fc5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17488.78919999999, "isoelectric_point": 9.542755317687988 }, "id": "JPCNHPFPBM_957", "locus": "GCA000292505_00957" }, { "type": "cds", "sequence": "contig_1", "start": 528573, "stop": 528764, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGTTAATAACCATCAATATTTACCGCTTCAAAAAATTAAGTTTAATTTATGTGAAGAGATAGATTTAAGAAATAAAAATAAGTGGTTATTTCCTAATTCATTTAGCATTTTTCTAAAACAAGATTTAAGTTATAAAAAAGATCAAAATTACTTTAATTTAGTGAATATACCTAAACAGTTAAAGTGA", "aa": "MLVNNHQYLPLQKIKFNLCEEIDLRNKNKWLFPNSFSIFLKQDLSYKKDQNYFNLVNIPKQLK", "aa_hexdigest": "47b12d9341e17987ac73c366ebaa765d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7716.933899999998, "isoelectric_point": 9.56325626373291 }, "id": "JPCNHPFPBM_958", "locus": "GCA000292505_00958" }, { "type": "cds", "sequence": "contig_1", "start": 528816, "stop": 528980, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAAGAAAATATCTCTTTTTGATAATCTAGAACAGTGGGTTGATTTGTCATTATTAATGATTCGTACCTCCTTTTTAGATGCTAACTATTTTGGGGTTGCAAAGCTATCATTATTTAAGTATGGTAACTATCTCATTGATAATTTAAATCAAGCACAATGA", "aa": "MLKKISLFDNLEQWVDLSLLMIRTSFLDANYFGVAKLSLFKYGNYLIDNLNQAQ", "aa_hexdigest": "bb841f550bd964534f6c153e74b74ce0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6317.288900000001, "isoelectric_point": 5.955214500427247 }, "id": "JPCNHPFPBM_959", "locus": "GCA000292505_00959" }, { "type": "cds", "sequence": "contig_1", "start": 529018, "stop": 530463, "strand": "-", "frame": 1, "gene": "rnjA", "product": "putative protein MG423", "db_xrefs": [ "COG:COG0595", "COG:J", "GO:0003723", "GO:0005886", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47662", "UniRef:UniRef90_P47662" ], "nt": "TTGTTCCATACAGTAGGATTTTTTCCTATTTACACAAGTTCAATAGGCGCTTCTATAATTAAAAGCAAAATTAATGAAAACAAACTTAATATTGCACGTGATAAATTGGAAATTCATGAATTAAAACCACTTGAAACAATCGAAATTTCTAATCATTCTATTACCCCTTTTAAAGTATCAAGTTCTTTACCATCATCATTTGGATTTGCTTTAAATACTGATAATGGTTACATCGTTTTTATTGATGATTTCATTGTTTTAAATGATAAGAACATTGCTTTTGAAAATCAACTAAATCAAATTATCCCTAAACTTTCTGACAATACATTGTTATTAATAACAGGAGTTGGTTTAGTTGGTAGAAACAGTGGTTTTACAACACCTAAACACAAGTCATTAGAACAGTTAAACAGGATCATAACTCCAGCCAAAGGCAGAATATTTGTAGCTTGTTATGATTCAAATGCTTACAGTGTTATGACTCTTGCCCAGATTGCAAGGATGCAAAATCGTCCTTTTATTATCTATTCACAATCATTTGTGCATCTCTTTAACACAATTGTGCGTCAAAAATTGTTTAATAACACGCATTTAAATACCATTTCTATTGAGGAGATTAACAATTCAACCAACAGTATTGTTGTTCTCACCTCACCACCTGATAAGTTGTATGCTAAATTATTCAAAATTGGAATGAACGAAGATGAAAGAATTCGTTATCGTAAGAGTGATACTTTTATCTTTATGACTCCTAAAGTTGCTGGCTATGAAGAGATAGAAGCACAAATCCTTGATGATATTGCACGTAATGAAGTTAGTTATTACAACTTAGGTAGAGAAATCCTTTCTATCCAAGCAAGTGATGAAGATATGAAGTTTTTAGTTAGTTCTTTAAAACCAAAATATATTATTCCAACTGGTGGACTTTATAGGGATTTTATAAACTTCACAATGGTTTTAAAACAAGCTGGCGCTGAGCAAAATCAAATCTTAATTCCTTTTAACGGTGAAGTTTTAACTATTGAAAACAAAAAACTAGATAGCAAAAAAAATGAATTGAAATTAAATCCTAAGTGTGTTGATTCTGCTGGATTACAAGAAATTGGAGCTAGCATTATGTTTGAACGTGATCAAATGTCAGAATCTGGAGTTGTGATCATAATCATTTATTTTGATCAAAAAAAGAGTGAATTCTTAAATGAGATAACTTATTCTTTTTTAGGAGTATCTTTAGATGTACCAGAAAAAGATAAACTAAAAACAAAAATGGAAGAATTAATTAAAAAACAAATTAATGATATTAAGGATTTCACCACGATTAAAAAACGAATAGGTAAAGAAATTTCAAAAGAATTGAAGGTTTCTATTAAACGAGCAGTAATGAATTTATTCACCAAGATGACATCAAAAGCACCCTTAATTCTTTCAACTATTATTTCTATTTAA", "aa": "MFHTVGFFPIYTSSIGASIIKSKINENKLNIARDKLEIHELKPLETIEISNHSITPFKVSSSLPSSFGFALNTDNGYIVFIDDFIVLNDKNIAFENQLNQIIPKLSDNTLLLITGVGLVGRNSGFTTPKHKSLEQLNRIITPAKGRIFVACYDSNAYSVMTLAQIARMQNRPFIIYSQSFVHLFNTIVRQKLFNNTHLNTISIEEINNSTNSIVVLTSPPDKLYAKLFKIGMNEDERIRYRKSDTFIFMTPKVAGYEEIEAQILDDIARNEVSYYNLGREILSIQASDEDMKFLVSSLKPKYIIPTGGLYRDFINFTMVLKQAGAEQNQILIPFNGEVLTIENKKLDSKKNELKLNPKCVDSAGLQEIGASIMFERDQMSESGVVIIIIYFDQKKSEFLNEITYSFLGVSLDVPEKDKLKTKMEELIKKQINDIKDFTTIKKRIGKEISKELKVSIKRAVMNLFTKMTSKAPLILSTIISI", "aa_hexdigest": "82c0da418d06d27d1112b8e0b90ba65e", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_P47662", "query_cov": 1.0, "subject_cov": 0.857397504456328, "identity": 0.996, "score": 915.0, "evalue": 0.0, "valid": true, "gene": "RnjA", "product": "Uncharacterized protein MG423", "uniref50_id": "UniRef50_P47662", "cog_id": "COG0595", "cog_category": "J", "go_ids": [ "GO:0005886", "GO:0046872", "GO:0003723" ] }, "pscc": { "uniref50_id": "UniRef50_P47662", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47662" ], "product": "Uncharacterized protein MG423" }, "genes": [ "rnjA" ], "id": "JPCNHPFPBM_960", "locus": "GCA000292505_00960" }, { "type": "cds", "sequence": "contig_1", "start": 530542, "stop": 530703, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002F5EBD4", "UniRef:UniRef90_UPI0002F5EBD4" ], "nt": "ATGGCTAAGATTAAATTCTTTGCACTCGGCGGTCAAGATGAACGTGGTAAAAATTGCTATGTGCTTGAAATTGACAATGATGTGTTTATCTTTAATGTTGGTAGCTTAACACCAACAACAGCAGTTTTAGGTGTTAAAAAAATTATTCCTGATTTTAGTTGA", "aa": "MAKIKFFALGGQDERGKNCYVLEIDNDVFIFNVGSLTPTTAVLGVKKIIPDFS", "aa_hexdigest": "c9b5373594c7ede41c36b5c1622af55e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0002F5EBD4", "query_cov": 1.0, "subject_cov": 0.803030303030303, "identity": 1.0, "score": 110.0, "evalue": 7.18e-31, "valid": true, "uniref50_id": "UniRef50_UPI0002F5EBD4" }, "pscc": { "uniref50_id": "UniRef50_UPI0002F5EBD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002F5EBD4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5821.718500000001, "isoelectric_point": 6.024103355407715 }, "id": "JPCNHPFPBM_961", "locus": "GCA000292505_00961" }, { "type": "cds", "sequence": "contig_1", "start": 530706, "stop": 530966, "strand": "-", "frame": 2, "gene": "rpsO", "product": "30S ribosomal protein S15", "db_xrefs": [ "COG:COG0184", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627", "RefSeq:WP_009885608.1", "SO:0001217", "UniParc:UPI0000134D0F", "UniRef:UniRef100_P47663", "UniRef:UniRef50_P47663", "UniRef:UniRef90_P47663" ], "nt": "ATGAAAATTGATAAAGAACAAATCATTAAGGCTCATCAACTTCACAAAAACGATGTTGGCAGTGTGCAAGTACAAATCTCTATATTAACAGATCAGATTAAAAAATTAACAGACCACCTGTTAGCAAACAAAAAGGATTTTATTTCTAAGCGTGGTTTATATACAAAGGTATCAAAAAGAAAACGGCTACTTAAATATCTAAAAGAACGTAATATTGAAACCTACCGTGATTTGATTAAAAATTTAAACCTCCGTGGTTAA", "aa": "MKIDKEQIIKAHQLHKNDVGSVQVQISILTDQIKKLTDHLLANKKDFISKRGLYTKVSKRKRLLKYLKERNIETYRDLIKNLNLRG", "aa_hexdigest": "6aeee8d790f9c955a6872e2df8a5c4fe", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000134D0F", "ncbi_nrp_id": "WP_009885608.1", "uniref100_id": "UniRef100_P47663", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000134D0F", "RefSeq:WP_009885608.1", "UniRef:UniRef100_P47663" ] }, "ips": { "uniref100_id": "UniRef100_P47663", "uniref90_id": "UniRef90_P47663" }, "psc": { "uniref90_id": "UniRef90_P47663", "gene": "rpsO", "product": "30S ribosomal protein S15", "uniref50_id": "UniRef50_P47663", "cog_id": "COG0184", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P47663", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47663" ], "product": "Small ribosomal subunit protein uS15" }, "genes": [ "rpsO" ], "id": "JPCNHPFPBM_962", "locus": "GCA000292505_00962" }, { "type": "cds", "sequence": "contig_1", "start": 531155, "stop": 531493, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00270.35" ], "nt": "ATGGACTCAACCTTTCATGAGCTTGGGATCTCTCAAACTTTAATTGAAACGCTTAATGCGCTTCATATTAATAAGCCAACAAAAATTCAACAAATCTCTATCCCTCAGTTTTTATCAGAAAAAAACTTAATAGTTCACTCGCCAACAGGAACTGGTAAAACTGCTGCTTTTGCAATTCCCATAATTGAGAAGCTATTAAAAGAAGATCAAACAGCAAAACCAACTTTAGTAATTGCTCCAACAAGAGAATTAGTAGAACAGATTAAAACCACATTTTCAAATATTGCTAAAAATAAAAAACTAAGAATTATTAGTTTAATTGGTGGTGTACCTGCTTGA", "aa": "MDSTFHELGISQTLIETLNALHINKPTKIQQISIPQFLSEKNLIVHSPTGTGKTAAFAIPIIEKLLKEDQTAKPTLVIAPTRELVEQIKTTFSNIAKNKKLRIISLIGGVPA", "aa_hexdigest": "50c2997dd1d88c2d2a506f2a77ce5c13", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00270.35", "name": "DEAD", "length": 84, "aa_cov": 0.75, "hmm_cov": 0.9880952380952381, "evalue": 2.3979875894157364e-25, "score": 84.29902648925781, "start": 27, "stop": 110 } ], "seq_stats": { "molecular_weight": 12266.26289999998, "isoelectric_point": 9.573248863220215 }, "id": "JPCNHPFPBM_963", "locus": "GCA000292505_00963" }, { "type": "cds", "sequence": "contig_1", "start": 531542, "stop": 532489, "strand": "+", "frame": 2, "gene": "srmB", "product": "putative ATP-dependent RNA helicase MG425", "db_xrefs": [ "COG:COG0513", "COG:L", "EC:3.6.4.13", "GO:0003689", "GO:0003724", "GO:0005524", "GO:0005829", "GO:0009409", "GO:0016887", "GO:0033592", "GO:0061775", "GO:0140584", "GO:0140665", "GO:0140849", "SO:0001217", "UniRef:UniRef50_P47664", "UniRef:UniRef90_P47664" ], "nt": "ATGGGTAGAATTATGGATCTTTTAGAGCGTAAAGCAATTCATTTTAACGATTTAGAACACCTAATTATTGATGAAGTTGATCTAATGTTAGACCGTGGTTTTAAAAAACAAATTTTTAATTTACTAGAACAAATCAATTCCTTTAAACAAATTGCTGTTTATTCAGCTAGTTACAACCAAGAAGCTATTAACATTGCCAAGCAAATTACTAATAATGGGATCTTTATTGGATCACCTGAATTTAATAAAGACGTAAATACCAATAATGATAAACTAATCAAACAATTTGTTTGTTATCTATTTTCAGATCAAAAAAAGCAAGCTTTATACAGCCTTATAAAAACAGCACAAGTTAAGTCAATCATTGTTTTTTGTGACACTAAAAAACTAGTTGATGATCTTCATGTATTTTTAAGAAAAAATGAATTAAGAACTTTTGCACTTCATGGTGATAAAAAACAATTTATTAGAGAGAGAAATCTTAAAATCTTTGCCAATACAAAACAACCCACTATTCTAGTAACTACTGATCTTATTGGTCGTGGTATCCATGTTGAAGCAATCGATATGGTTATCAATTATTCAGCTTGTTTAAATCTAGAAGCTTATATAAATAGAATGGGAAGGACTGGCAGAAACAATCATAAAGGGACATGTGTAACTTTCTGCACCTCACAAGAAAAGAAAGTCTTTCTGAAAATGGTTGAGAAAATCACTGATAATCGAATAGCTGAATGTAAACAAATGGAAATAAAGTTAATTCCTTTAAAAAATAAAGCTAAAACTAAAAAAGGTGGTATTTCACTTGATTGTGTTCAGAAAATATATGCCAATGCAAAACCATATGACCGTAATAAACGTGTCCCTTTAGCAAGTGATCTTTTCAAAAGTCGTATGCGCCAGCCTGAAAAAGCTATGCAAAAGCAAAAAATTCATGACAATGACTGA", "aa": "MGRIMDLLERKAIHFNDLEHLIIDEVDLMLDRGFKKQIFNLLEQINSFKQIAVYSASYNQEAINIAKQITNNGIFIGSPEFNKDVNTNNDKLIKQFVCYLFSDQKKQALYSLIKTAQVKSIIVFCDTKKLVDDLHVFLRKNELRTFALHGDKKQFIRERNLKIFANTKQPTILVTTDLIGRGIHVEAIDMVINYSACLNLEAYINRMGRTGRNNHKGTCVTFCTSQEKKVFLKMVEKITDNRIAECKQMEIKLIPLKNKAKTKKGGISLDCVQKIYANAKPYDRNKRVPLASDLFKSRMRQPEKAMQKQKIHDND", "aa_hexdigest": "ee10e221ff6cd36592e7e874b19ad071", "start_type": "ATG", "rbs_motif": null, "genes": [ "srmB" ], "truncated": "both", "pseudogene": { "start": 531242, "stop": 532504, "observations": { "mutation": [ 531491, 532487 ], "stop": [ 531491, 532487 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47664", "query_cov": 1.0, "subject_cov": 0.7015590200445434, "identity": 0.9940000000000001, "score": 615.0, "evalue": 4.22e-220, "gene_start": 1, "gene_end": 315, "reference_start": 130, "reference_end": 444, "reference_sequence": "MDSTFHELGISQTLIETLNALHINKPTKIQQISIPQFLSEKNLIVHSPTGTGKTAAFAIPIIEKLLKEDQTAKPTLVIAPTRELVEQIKTTFSNIAKNKKLRIISLIGGVPAWKQIKKIKTNPQIIVGTMGRIMDLLERKAIHFSDLEHLIIDEVDLMLDRGFKKQIFNLLEQINSFKQIAVYSASYNQEAINIAKQITNNGIFIGSPEFNKDANTNNDKLIKQFVCYLFSDQKKQALYSLIKTAQVKSIIVFCDTKKLVDDLHVFLRKNELRTFALHGDKKQFIRERNLKIFANTKQPTILVTTDLIGRGIHVEAIDMVINYSACLNLEAYINRMGRTGRNNHKGTCVTFCTSQEKKVFLKMVEKITDNRIAECKQMEIKLIPLKNKAKTKKGGISLDCVQKIYANAKPYDRNKRVPLASDLFKSRMRQPEKAMQKQKIHDNDWQSNM" }, "paralog": false, "identity": 0.9904761904761905, "score": 815.0, "evalue": 9.71e-302, "description": "Internal stop codon at 531491, 532487. Nonsense mutation around 531491, 532487.", "psc": { "uniref90_id": "UniRef90_P47664", "gene": "SrmB", "product": "Probable ATP-dependent RNA helicase MG425", "ec_ids": [ "3.6.4.13" ], "uniref50_id": "UniRef50_P47664", "cog_id": "COG0513", "cog_category": "L", "go_ids": [ "GO:0005829", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003689", "GO:0003724", "GO:0033592", "GO:0009409" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47664", "EC:3.6.4.13", "UniRef:UniRef50_P47664", "COG:COG0513", "COG:L", "GO:0005829", "GO:0005524", "GO:0016887", "GO:0140849", "GO:0140665", "GO:0140584", "GO:0061775", "GO:0003689", "GO:0003724", "GO:0033592", "GO:0009409" ] }, "pscc": { "uniref50_id": "UniRef50_P47664", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47664" ], "product": "Probable ATP-dependent RNA helicase MG425" } }, "id": "JPCNHPFPBM_964", "locus": "GCA000292505_00964" }, { "type": "cds", "sequence": "contig_1", "start": 532527, "stop": 532721, "strand": "+", "frame": 3, "gene": "rpmB", "product": "50S ribosomal protein L28", "db_xrefs": [ "COG:COG0227", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_009885606.1", "SO:0001217", "UniParc:UPI0000133E3F", "UniRef:UniRef100_P47665", "UniRef:UniRef50_Q6MTC5", "UniRef:UniRef90_P47665" ], "nt": "ATGGCAAAAAAAGACCAACTTACCTTAAGAGGGCCTTTGTATGGCAATAATCGTTCTCATTCCAAAACTATTACAAGAAGAAAATGGAATGTAAACCTTCAGTCATGCAAAATTAAAGATACTAATGGTAAAGTAACACGGATTCTAGTTTCAACTAAAACAATTCGTACCCTTAAAAAACAAAATCGTTTCTAA", "aa": "MAKKDQLTLRGPLYGNNRSHSKTITRRKWNVNLQSCKIKDTNGKVTRILVSTKTIRTLKKQNRF", "aa_hexdigest": "e60ec7143344c99d1a5fc9f7fa1f4c04", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133E3F", "ncbi_nrp_id": "WP_009885606.1", "uniref100_id": "UniRef100_P47665", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133E3F", "RefSeq:WP_009885606.1", "UniRef:UniRef100_P47665" ] }, "ips": { "uniref100_id": "UniRef100_P47665", "uniref90_id": "UniRef90_P47665" }, "psc": { "uniref90_id": "UniRef90_P47665", "gene": "rpmB", "product": "50S ribosomal protein L28", "uniref50_id": "UniRef50_Q6MTC5", "cog_id": "COG0227", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q6MTC5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MTC5" ], "product": "Large ribosomal subunit protein bL28" }, "genes": [ "rpmB" ], "id": "JPCNHPFPBM_965", "locus": "GCA000292505_00965" }, { "type": "cds", "sequence": "contig_1", "start": 532757, "stop": 533047, "strand": "+", "frame": 2, "gene": "yhfA", "product": "Hydroperoxide reductase", "db_xrefs": [ "COG:COG1765", "COG:R", "EC:1.11.1.-", "GO:0004601", "GO:0005737", "SO:0001217", "UniRef:UniRef50_P47666", "UniRef:UniRef90_P47666" ], "nt": "ATGGATAAAAAATACGATATCACAGCTGTTTTGAACGATGATAGTTCTATTAATGCAGTAAGCGATAACTTCCAAATAACACTGGATGCACGACCAAAAGAAAAATCTAAAGGCATTAATCCTCTGTCAGCTTTTTTGGCTGGTTTAGCTGCATGTGAACTTGCAACTGCTAATGCGATGGCAGCTGCTAAGATGATAACTTTGAACAAGGCACTGATTAACATCAAAGGTTATCGTTTAACAAATCCTAGTGATGGTTATTTTGGCCTACGTGAACTTAACATTCACTGA", "aa": "MDKKYDITAVLNDDSSINAVSDNFQITLDARPKEKSKGINPLSAFLAGLAACELATANAMAAAKMITLNKALINIKGYRLTNPSDGYFGLRELNIH", "aa_hexdigest": "e50dd3359b8c28aa987740bc630a4faf", "start_type": "ATG", "rbs_motif": null, "genes": [ "yhfA" ], "truncated": "3-prime", "pseudogene": { "start": 532757, "stop": 533182, "observations": { "mutation": [ 533045 ], "stop": [ 533045 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47666", "query_cov": 1.0, "subject_cov": 0.6808510638297872, "identity": 1.0, "score": 185.0, "evalue": 1.09e-58, "gene_start": 1, "gene_end": 96, "reference_start": 1, "reference_end": 96, "reference_sequence": "MDKKYDITAVLNDDSSINAVSDNFQITLDARPKEKSKGINPLSAFLAGLAACELATANAMAAAKMITLNKALINIKGYRLTNPSDGYFGLRELNIHWEIHSPNEEEEIKEFIDFVSKRCPAHNTLHGTSNFKINISVTLVH" }, "paralog": false, "identity": 0.9929078014184397, "score": 278.0, "evalue": 4.24e-98, "description": "Internal stop codon at 533045. Nonsense mutation around 533045.", "psc": { "uniref90_id": "UniRef90_P47666", "gene": "YhfA", "product": "Hydroperoxide reductase", "ec_ids": [ "1.11.1.-" ], "uniref50_id": "UniRef50_P47666", "cog_id": "COG1765", "cog_category": "R", "go_ids": [ "GO:0005737", "GO:0004601" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47666", "EC:1.11.1.-", "UniRef:UniRef50_P47666", "COG:COG1765", "COG:R", "GO:0005737", "GO:0004601" ] }, "pscc": { "uniref50_id": "UniRef50_P47666", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47666" ], "product": "Hydroperoxide reductase" } }, "id": "JPCNHPFPBM_966", "locus": "GCA000292505_00966" }, { "type": "cds", "sequence": "contig_1", "start": 533512, "stop": 533808, "strand": "+", "frame": 1, "gene": "gerE", "product": "putative protein MG428", "db_xrefs": [ "COG:COG5905", "COG:DK", "GO:0003700", "GO:0006352", "SO:0001217", "UniRef:UniRef50_P47667", "UniRef:UniRef90_P47667" ], "nt": "ATGGTGAATAAAGTTGAATTGCTTGATTCTAAAAGTAAATACTCATTAGAAAAGCAAAACACAGTTCTAAATACACCTGAAAACTATTTACGGAGTTTAGAATTTAAAGAAATTATTACTAAAGCTTTTAATAAAGCTAAAAACGATCAAGAGAGAAAAGTTTTTTCTTTATATGTAAAGGGCTATAAAAACTTTGAGATAGCAAAAAAGCTCAATATTAGTCCTAGAAGAGTGAGATATTTATTAGATCTTTTTAAAAGCTACATCAAATTGCTAACAGAAAGATATGGATATTAA", "aa": "MVNKVELLDSKSKYSLEKQNTVLNTPENYLRSLEFKEIITKAFNKAKNDQERKVFSLYVKGYKNFEIAKKLNISPRRVRYLLDLFKSYIKLLTERYGY", "aa_hexdigest": "a60561533f3a89e19fe4e179790c3501", "start_type": "ATG", "rbs_motif": null, "genes": [ "gerE" ], "truncated": "5-prime", "pseudogene": { "start": 533293, "stop": 533808, "observations": { "mutation": [ 533347, 533356 ], "stop": [ 533347, 533356 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47667", "query_cov": 1.0, "subject_cov": 0.5730994152046783, "identity": 1.0, "score": 188.0, "evalue": 1.88e-59, "gene_start": 1, "gene_end": 98, "reference_start": 74, "reference_end": 171, "reference_sequence": "MKNNISDVKLGLLAAKIYWKSWRFLELTEDDIISIALHAEQDSKKRFNPEFGLSFDNYLKLNGANFIRSSFRSMVNKVELLDSKSKYSLEKQNTVLNTPENYLRSLEFKEIITKAFNKAKNDQERKVFSLYVKGYKNFEIAKKLNISPRRVRYLLDLFKSYIKLLTERYGY" }, "paralog": false, "identity": 0.9883040935672515, "score": 328.0, "evalue": 3.13e-117, "description": "Internal stop codon at 533347, 533356. Nonsense mutation around 533347, 533356.", "psc": { "uniref90_id": "UniRef90_P47667", "gene": "GerE", "product": "Uncharacterized protein MG428", "uniref50_id": "UniRef50_P47667", "cog_id": "COG5905", "cog_category": "DK", "go_ids": [ "GO:0003700", "GO:0006352" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47667", "UniRef:UniRef50_P47667", "COG:COG5905", "COG:DK", "GO:0003700", "GO:0006352" ] }, "pscc": { "uniref50_id": "UniRef50_P47667", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47667" ], "product": "Uncharacterized protein MG428" } }, "id": "JPCNHPFPBM_967", "locus": "GCA000292505_00967" }, { "type": "cds", "sequence": "contig_1", "start": 533805, "stop": 534035, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02896.24" ], "nt": "ATGTGTGGTGAAATGGCCAGTGATCAATATGCAATACCGTTGCTTTTAGGTTTAGGGTTAACAGAGTTATCAATGAGTGCAAGTTCAATGTTTAAAGCAAGAATGGTTATTGCTAAAATAACAATTAATGAATGTAAATCATTAGTTGAAAAAGCATTGAAATTAACTAGTGATAGTGCTGTTAGAAAACTAGTAGAAAATTTCTTTAAAAAGAAAAATATCTTTATTTAA", "aa": "MCGEMASDQYAIPLLLGLGLTELSMSASSMFKARMVIAKITINECKSLVEKALKLTSDSAVRKLVENFFKKKNIFI", "aa_hexdigest": "7507b1111836ac45260a9c1b52b55ee4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02896.24", "name": "PEP-utilizers_C", "length": 40, "aa_cov": 0.5263157894736842, "hmm_cov": 1.0, "evalue": 3.754883640228163e-11, "score": 37.77103805541992, "start": 1, "stop": 40 } ], "seq_stats": { "molecular_weight": 8417.043499999992, "isoelectric_point": 9.301256752014158 }, "id": "JPCNHPFPBM_968", "locus": "GCA000292505_00968" }, { "type": "cds", "sequence": "contig_1", "start": 534042, "stop": 534539, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02896.24" ], "nt": "TTGGTAATAATTAGAACATTAGACATTGGTGGAGATAAAAAACTAAATTATTTTCAATTTCCTCATGAAGATAACCCTTTCTTAGGTTATCGTGCTATCCGTTTAACATTAGATAAACAAGCTGTTTTTAAGACCCAACTTCGTGCTTTATTAAGAGCTTCTGATTATGGAAATTTAGGAATTATGTTTCCAATGGTTGCAACTCTTGATGAATTAGTTCAAGTTAAGCAACTTTTAACAAAAGTTCAACAAGAATTTAATGAAACTAAGAAATTTAAATTAGGAATAATGATTGAAATTCCATCAGCAGCATTAGCAGCTGATTGCTTAGGAAAACATGTTGATTTTTTCTCAATAGGTAGCAATGATTTAATCCAATATAGTTTTGCTGCTGATAGGATGAATAAAAACGTTAGTTATCTATACCAACCATTAAATCCAGCATTACTACGCTTAATTAAACTAGTTGTAGAAGGTGGTAAATTAAATAATGTTTGA", "aa": "MVIIRTLDIGGDKKLNYFQFPHEDNPFLGYRAIRLTLDKQAVFKTQLRALLRASDYGNLGIMFPMVATLDELVQVKQLLTKVQQEFNETKKFKLGIMIEIPSAALAADCLGKHVDFFSIGSNDLIQYSFAADRMNKNVSYLYQPLNPALLRLIKLVVEGGKLNNV", "aa_hexdigest": "5720b2644b7a7a4a680dc830d9719160", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02896.24", "name": "PEP-utilizers_C", "length": 164, "aa_cov": 0.9636363636363636, "hmm_cov": 1.0, "evalue": 4.91308511659323e-70, "score": 231.05059814453125, "start": 2, "stop": 160 } ], "seq_stats": { "molecular_weight": 18667.6638, "isoelectric_point": 9.161424827575683 }, "id": "JPCNHPFPBM_969", "locus": "GCA000292505_00969" }, { "type": "cds", "sequence": "contig_1", "start": 534600, "stop": 534788, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02896.24" ], "nt": "TTGGAATCCGACTTTCAAAATGAATTAAAACAATATACCAATAAATTAGTAAAAACCTTAGATGGATATGAAGTTATTGTAGCTTCTAATATTGGTAATGTAAAAGATATGGATTTAGCAGTTGAGTATAACACGAATGGAATTGGTCTTTTTAGAACTGAATTTCTCTATATGAGTAGTCAAGATTGA", "aa": "MESDFQNELKQYTNKLVKTLDGYEVIVASNIGNVKDMDLAVEYNTNGIGLFRTEFLYMSSQD", "aa_hexdigest": "501957243ce3987a11dce3817690f6e0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02896.24", "name": "PEP-utilizers_C", "length": 54, "aa_cov": 0.8709677419354839, "hmm_cov": 1.0, "evalue": 1.1063652226515994e-11, "score": 39.51301193237305, "start": 8, "stop": 61 } ], "seq_stats": { "molecular_weight": 7101.8618, "isoelectric_point": 4.361221885681153 }, "id": "JPCNHPFPBM_970", "locus": "GCA000292505_00970" }, { "type": "cds", "sequence": "contig_1", "start": 534804, "stop": 535523, "strand": "-", "frame": 2, "gene": "ptsI", "product": "Phosphoenolpyruvate-protein phosphotransferase", "db_xrefs": [ "COG:COG1080", "COG:G", "EC:2.7.3.9", "GO:0005737", "GO:0008965", "GO:0009401", "GO:0016301", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P47668", "UniRef:UniRef90_P47668" ], "nt": "ATGAAAAAAATAATTGGGATTGGTGTTTCAGATGGCATTGCTGTTGCAAAAGCTTTCATTATTCAAACACCTCAATTTGACGTTAAAAAATACACAAATGTCAAAATGACACCCACCCAAGCAAAAAAGCTTTTAAGCAGTGCTTTTCAAAAAGCAAAAAAAGACCTTGAAGAGATTAAGACAATTACTGTTAAGAATATTAATCAAGAAGCAGGAATGATTTTTGATGCACACATCCAAATTCTTAATGATCCTACTATCACTGAGCAATTAGAACAACAACTAAATAAAAACATCCATCCTGTTATTGCTGTTGATAATGTTTTTCAACAAACAACTTTAATGTTTAGTGAAATGGATGATAAGTATTTTAAAGAACGTGCCAGTGATATATTAGATTTACACCAAAGGCTCTTAAGTTATTTAACTGGAGTAAAACTTAATGATTTAATCAGGATTAAAAGTGATGTCATTATAGTAGCTAATGATTTAACACCAAGTCAAACAGCAACTCTTAATAAAAAGTATGTCAAGGGTTTTTTAACTGAGAGTGGTGGTAAAACAAGTCATGCTGCCATTATGGCTCGTTCTATGGAAATTCCTGCCATAGTTGGTTTAAAAAATATTACCAGTAAAGTTGAAGATGGCAAAACAGTTGGTATCAATGGTAGAAAAGGTATTGTGGGTTTTGACTTTTCAAGTAAAGACATAACTCAATGA", "aa": "MKKIIGIGVSDGIAVAKAFIIQTPQFDVKKYTNVKMTPTQAKKLLSSAFQKAKKDLEEIKTITVKNINQEAGMIFDAHIQILNDPTITEQLEQQLNKNIHPVIAVDNVFQQTTLMFSEMDDKYFKERASDILDLHQRLLSYLTGVKLNDLIRIKSDVIIVANDLTPSQTATLNKKYVKGFLTESGGKTSHAAIMARSMEIPAIVGLKNITSKVEDGKTVGINGRKGIVGFDFSSKDITQ", "aa_hexdigest": "6ab6d2b536cdea77b1752ff6e1fe0fca", "start_type": "ATG", "rbs_motif": null, "genes": [ "ptsI" ], "truncated": "5-prime", "pseudogene": { "start": 534501, "stop": 535523, "observations": { "mutation": [ 534602, 534806 ], "stop": [ 534602, 534806 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47668", "query_cov": 1.0, "subject_cov": 0.4178321678321678, "identity": 0.996, "score": 456.0, "evalue": 7.67e-157, "gene_start": 1, "gene_end": 239, "reference_start": 1, "reference_end": 239, "reference_sequence": "MKKIIGIGVSDGIAVAKAFIIQTPQFDVKKYTNVKMTPTQAKKLLSSAFQKAKKDLEEIKTITVKNINQEAGMIFDAHIQILNDPTITEQLEQQLNKNIHPVIAVDNVFQQTALMFSEMDDKYFKERASDILDLHQRLLSYLTGVKLNDLIRIKSDVIIVANDLTPSQTATLNKKYVKGFLTESGGKTSHAAIMARSMEIPAIVGLKNITSKVEDGKTVGINGRKGIVGFDFSSKDITQWKQEKELESNFQNELKQYTNKLVKTLDGYEVIVASNIGNVKDMDLAVEYNTNGIGLFRTEFLYMSSQDWPDESVQFEAYKTVLQKAKNDLVIIRTLDIGGDKKLNYFQFPHEDNPFLGYRAIRLTLDKQAVFKTQLRALLRASDYGNLGIMFPMVATLDELVQVKQLLTKVQQEFNETKKFKLGIMIEIPSAALAADCLGKHVDFFSIGSNDLIQYSFAADRMNKNVSYLYQPLNPALLRLIKLVVEGGKLNNVWTGMCGEMASDQYAIPLLLGLGLTELSMSASSMFKARMVIAKITINECKSLVEKALKLTSDSAVRKLVENFFKKKNIFI" }, "paralog": false, "identity": 0.9882352941176471, "score": 647.0, "evalue": 9.71e-235, "description": "Internal stop codon at 534602, 534806. Nonsense mutation around 534602, 534806.", "psc": { "uniref90_id": "UniRef90_P47668", "gene": "ptsI", "product": "Phosphoenolpyruvate-protein phosphotransferase", "ec_ids": [ "2.7.3.9" ], "uniref50_id": "UniRef50_P47668", "cog_id": "COG1080", "cog_category": "G", "go_ids": [ "GO:0005737", "GO:0016301", "GO:0046872", "GO:0008965", "GO:0009401" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47668", "EC:2.7.3.9", "UniRef:UniRef50_P47668", "COG:COG1080", "COG:G", "GO:0005737", "GO:0016301", "GO:0046872", "GO:0008965", "GO:0009401" ] }, "pscc": { "uniref50_id": "UniRef50_P47668", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47668" ], "product": "Phosphoenolpyruvate-protein phosphotransferase" } }, "id": "JPCNHPFPBM_971", "locus": "GCA000292505_00971" }, { "type": "cds", "sequence": "contig_1", "start": 535527, "stop": 537050, "strand": "-", "frame": 2, "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "db_xrefs": [ "COG:COG0696", "COG:G", "EC:5.4.2.12", "GO:0005829", "GO:0005975", "GO:0006007", "GO:0006096", "GO:0030145", "GO:0046537", "KEGG:K15633", "RefSeq:WP_009885603.1", "SO:0001217", "UniParc:UPI0000557C28", "UniRef:UniRef100_P47669", "UniRef:UniRef50_P75167", "UniRef:UniRef90_P47669" ], "nt": "ATGCATAAAAAAGTTTTATTAGCAATCCTTGATGGTTATGGGATCTCAAATGCTATTTATGGTAATGCAGTACAAAATGCAAATACCCCAATGCTAGATGAATTAATCAATTCATATCCTTGTGTACTTTTAGATGCATCTGGGGAAGCAGTTGGATTGCCTATGGGTCAAATAGGTAACTCTGAGGTAGGTCATCTAAATATTGGGGCAGGTCGAGTTGTTTATACTGGACTTTCTTTGATTAATCAACATATTAAGGATCGTAGTTTTTTTGCAAATAAAGCTTTTTTAAAAACCATAGAACATGTAGAAAAAAACCATTCAAAAATCCATTTAATTGGGTTATTTTCCAATGGAGGAGTGCATAGTCATAATGAACATCTATTAGCACTCATTGAATTGTTTTCAAAACATGCAAAGGTAGTATTACATTTATTTGGTGATGGTAGAGATGTAGCACCTTGTAGCTTAAAACAAGATCTTGAGAAATTAATGATATTTCTAAAAAACTATCCTAATGTTGTTATTGGAACTATTGGGGGAAGATACTATGGAATGGATCGTGATCAACGCTGGGATCGTGAAATGATTGCTTATAAAGCTTTATTAGGAGTTTCAAAAAATAAATTCAATGACCCAATTGGTTATATTGAAACGCAATATCAGAACCAAATTACTGATGAATTTATTTATCCTGCAATTAATGCCAATTTAAATTCTGATCAGTTTGCATTAAACAATAATGATGGAGTTATTTTCTTTAATTTTAGACCTGATAGAGCAAGACAAATGTCCCATTTGATCTTTAACAGCAATTATTACAACTATCAACCTGAATTGAAACGAAAAGAAAATTTATTTTTTGTAACAATGATGAATTATGAGGGAATTGTACCTAGCGAATTTGCTTTTCCACCTCAAACCATTAAAAATAGTCTTGGTGAAGTAATTGCTAATAATAATTTGAAGCAATTGAGGATTGCAGAAACTGAAAAGTATGCTCACGTTACTTTCTTTTTTGATGGTGGTTTTGAAGTTAATCTCAGCAATGAAACAAAGACATTAATTCCTTCTTTAAAAGTTGCTACATATGATTTAGCTCCCGAAATGTCATGTAAAGCTATTACTGATGCACTACTAGAAAAGCTTAATAACTTTGATTTTACTGTTTTAAATTTTGCTAATCCTGATATGGTAGGTCATACTGGTAACTATCAAGCTTGCATTAAAGCTCTTGAAGCACTCGATGTTCAAATTAAACGAATAGTTGATTTTTGTAAAGCTAATCAAATAACTATGTTTTTAACTGCAGATCATGGGAATGCAGAAGTGATGATTGATAATAATAACAATCCAGTTACTAAACACACTATTAATCCTGTACCATTTGTATGTACTGACAAAAATGTTAACTTTAATCAAACTGGAATTTTAGCTAATATTGCTCCTACTATCTTGGAATATCTTAACCTTAGCAAACCAAAAGAGATGACTGCAAAATCCTTATTAAAAAATAACAATTAA", "aa": "MHKKVLLAILDGYGISNAIYGNAVQNANTPMLDELINSYPCVLLDASGEAVGLPMGQIGNSEVGHLNIGAGRVVYTGLSLINQHIKDRSFFANKAFLKTIEHVEKNHSKIHLIGLFSNGGVHSHNEHLLALIELFSKHAKVVLHLFGDGRDVAPCSLKQDLEKLMIFLKNYPNVVIGTIGGRYYGMDRDQRWDREMIAYKALLGVSKNKFNDPIGYIETQYQNQITDEFIYPAINANLNSDQFALNNNDGVIFFNFRPDRARQMSHLIFNSNYYNYQPELKRKENLFFVTMMNYEGIVPSEFAFPPQTIKNSLGEVIANNNLKQLRIAETEKYAHVTFFFDGGFEVNLSNETKTLIPSLKVATYDLAPEMSCKAITDALLEKLNNFDFTVLNFANPDMVGHTGNYQACIKALEALDVQIKRIVDFCKANQITMFLTADHGNAEVMIDNNNNPVTKHTINPVPFVCTDKNVNFNQTGILANIAPTILEYLNLSKPKEMTAKSLLKNNN", "aa_hexdigest": "097c042439f6da10ae27224b1b6e3ca6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000557C28", "ncbi_nrp_id": "WP_009885603.1", "uniref100_id": "UniRef100_P47669", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000557C28", "RefSeq:WP_009885603.1", "UniRef:UniRef100_P47669" ] }, "ips": { "uniref100_id": "UniRef100_P47669", "uniref90_id": "UniRef90_P47669" }, "psc": { "uniref90_id": "UniRef90_P47669", "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "ec_ids": [ "5.4.2.12" ], "uniref50_id": "UniRef50_P75167", "cog_id": "COG0696", "cog_category": "G", "kegg_orthology_id": "K15633", "go_ids": [ "GO:0005829", "GO:0005975", "GO:0006007", "GO:0006096", "GO:0030145", "GO:0046537" ] }, "pscc": { "uniref50_id": "UniRef50_P75167", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75167" ], "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase" }, "genes": [ "gpmI" ], "id": "JPCNHPFPBM_972", "locus": "GCA000292505_00972" }, { "type": "cds", "sequence": "contig_1", "start": 537289, "stop": 537777, "strand": "-", "frame": 1, "gene": "tpiA", "product": "triose-phosphate isomerase", "db_xrefs": [ "COG:COG0149", "COG:G", "EC:5.3.1.1", "GO:0004807", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0019563", "GO:0046166", "KEGG:K01803", "SO:0001217", "UniRef:UniRef50_P78010", "UniRef:UniRef90_P47670" ], "nt": "ATGCGCACAAGGTATTTAATTGGCAATTGGAAAACAAATAAAAATTTAAAAGACGCAGTTAGTTTTGTTGAACAATTTCAACAAAATAAACTTAATTACAATGCCAAAATTGGGATAGCACCTGTTTATGTTCATCTCACTGAAATAAAAAAAATAATTAGTGATAGTCTCCTTTTATTTGCCCAAGACGCTAACTTTATTGAAAGTGGTTCATATACTGGAACTGTAAGCTTTACTCAACTTCAAGACATTGGTGTTAACAACAGTATTATTGGTCATTCTGAAAGAAGAAAATACTATAACGAAACCAGTGCAGTTATTAATCAAAAGCTCTTTGCTTGTCTAAAAGCATCCATGCAAGTAGTTTTATGTATTGGTGAGACTTTAGGACAAGAGATTAGCTTTCTTAAAACTGATCTTACTAATTGCTTAAATACGATTGACAAAAGCTTAATTAAAAATTTAGTTATTGCTTATGAACCTTTGTGA", "aa": "MRTRYLIGNWKTNKNLKDAVSFVEQFQQNKLNYNAKIGIAPVYVHLTEIKKIISDSLLLFAQDANFIESGSYTGTVSFTQLQDIGVNNSIIGHSERRKYYNETSAVINQKLFACLKASMQVVLCIGETLGQEISFLKTDLTNCLNTIDKSLIKNLVIAYEPL", "aa_hexdigest": "319511be7aaf42f2cf8922e4ea9ebf6f", "start_type": "ATG", "rbs_motif": null, "genes": [ "tpiA" ], "truncated": "5-prime", "pseudogene": { "start": 537043, "stop": 537777, "observations": { "mutation": [ 537291 ], "stop": [ 537291 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47670", "query_cov": 1.0, "subject_cov": 0.6639344262295082, "identity": 0.988, "score": 317.0, "evalue": 4.15e-108, "gene_start": 1, "gene_end": 162, "reference_start": 1, "reference_end": 162, "reference_sequence": "MRTRYLIGNWKTNKNLKDAVSFVEQFQQNKLNYNAKIGIAPVYVHLTEIKKIISDSLLLFAQDANFIESGSYTGTVSFTQLQDIGVNNSIIGHSERRKYYNETSAVINQKLFACLKASMQVVLCIGEALGQEISFLKTDLTNCLDTIDKSLIKNLVIAYEPLWAIGTGKTATPEVANQTIKTIREYINDLYDENVANNISILYGGSVDHNNIQKLAIMEQIDGFLVGKASLEIKNFLEMARVYA" }, "paralog": false, "identity": 0.9877049180327869, "score": 472.0, "evalue": 8.26e-172, "description": "Internal stop codon at 537291. Nonsense mutation around 537291.", "psc": { "uniref90_id": "UniRef90_P47670", "gene": "tpiA", "product": "triose-phosphate isomerase", "ec_ids": [ "5.3.1.1" ], "uniref50_id": "UniRef50_P78010", "cog_id": "COG0149", "cog_category": "G", "kegg_orthology_id": "K01803", "go_ids": [ "GO:0004807", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0019563", "GO:0046166" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47670", "EC:5.3.1.1", "UniRef:UniRef50_P78010", "COG:COG0149", "COG:G", "KEGG:K01803", "GO:0004807", "GO:0005829", "GO:0006094", "GO:0006096", "GO:0019563", "GO:0046166" ] }, "pscc": { "uniref50_id": "UniRef50_P78010", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78010" ], "product": "Triosephosphate isomerase" } }, "id": "JPCNHPFPBM_973", "locus": "GCA000292505_00973" }, { "type": "cds", "sequence": "contig_1", "start": 537837, "stop": 538049, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10035.14" ], "nt": "ATGTTTGAAGCTGAAGGAGGATATTCACGCCAAAAAACCCAAGTGCTTGTAACTAATTGTTTGCTTATTAAAGCTGCTAAGCTTTTAGAAGATGTGAGAAAGTTTGATCGTGATGCTTTGTTTTCCATTACTTTTATTAAAAAGCTTGATGGCTATATCTATGATAGACGTGCCAATAAGCAAACAAAACATGGAACTGAAAACAAGTCATAA", "aa": "MFEAEGGYSRQKTQVLVTNCLLIKAAKLLEDVRKFDRDALFSITFIKKLDGYIYDRRANKQTKHGTENKS", "aa_hexdigest": "68f12019e67831fc9cb12e3f9ddd37d1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10035.14", "name": "DUF2179", "length": 49, "aa_cov": 0.7, "hmm_cov": 1.0, "evalue": 2.734845316933948e-09, "score": 31.878143310546875, "start": 1, "stop": 49 } ], "seq_stats": { "molecular_weight": 8113.248599999996, "isoelectric_point": 9.560741996765138 }, "id": "JPCNHPFPBM_974", "locus": "GCA000292505_00974" }, { "type": "cds", "sequence": "contig_1", "start": 538062, "stop": 538337, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAATTAATACAGTTAGCTTTATTATTGGCTATGTAATTGGTACTTATTTAACTGGTAGTTTATCAGTGCAATCTTATGTTGGTGATGATAAACACCAGCCATTTGGAGTTGCTTTCTTTTTATCTCCAAACCTTGTTTTTACTCTATTGATGAACATTGTGTTGGGATTATTTACTTCTTTTTATTTCCCTAAGTATCAATTTGTAAAAGTAGAAGTTTATGGTAAGCACATAGAAAAAATTCGTAACTATCTACTTGATAATCAACAGTGA", "aa": "MLINTVSFIIGYVIGTYLTGSLSVQSYVGDDKHQPFGVAFFLSPNLVFTLLMNIVLGLFTSFYFPKYQFVKVEVYGKHIEKIRNYLLDNQQ", "aa_hexdigest": "a3d3a56c52259c9c7a9bcb3893067ea4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10451.071599999992, "isoelectric_point": 7.983909797668457 }, "id": "JPCNHPFPBM_975", "locus": "GCA000292505_00975" }, { "type": "cds", "sequence": "contig_1", "start": 538509, "stop": 538841, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTCCATTAGCCAAGGATTAGCTCGTCTTGTTAATTTTTTTATTACTTCACAAAATATTAATGTTGATTCAGCAACTATATTTAATGCTATTTTTTGGTTAACACAAATTCTTTTTAATGTTCCTTTCTTTATTTTTGGATGGTTTAAAATCTCCAAAAAATTTACCCTATTAACCCTTTATTTTGTAGCTGTTTCAAATCTTTTTGGTTTTTTCTTTTCATACATCCCTGGAATAGATAACTTCTTTTTGTTTGCAAATTTAACTACAGCAAAAGATGGTGGATTTGAAAATTTAATTAATGAAAAGGGAGTACAACTTATATTTTGA", "aa": "MASISQGLARLVNFFITSQNINVDSATIFNAIFWLTQILFNVPFFIFGWFKISKKFTLLTLYFVAVSNLFGFFFSYIPGIDNFFLFANLTTAKDGGFENLINEKGVQLIF", "aa_hexdigest": "d07475790e5d554120dc696c3924d2e9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12564.50529999998, "isoelectric_point": 8.063850593566894 }, "id": "JPCNHPFPBM_976", "locus": "GCA000292505_00976" }, { "type": "cds", "sequence": "contig_1", "start": 539119, "stop": 539235, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAACGAAAATAGAGCTAATAAAAGAATTGCGTAAATCAACACAAGCAAGTGTTATGGATTGTAAACAAGCTTTGGAAAAAAATAATGATGATTTTGAGAAAGCTGTTAAGTGA", "aa": "MATKIELIKELRKSTQASVMDCKQALEKNNDDFEKAVK", "aa_hexdigest": "a22b1c8f6cd0bba9263dc05eb1fb1408", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4355.0216, "isoelectric_point": 7.870574378967285 }, "id": "JPCNHPFPBM_977", "locus": "GCA000292505_00977" }, { "type": "cds", "sequence": "contig_1", "start": 539332, "stop": 539724, "strand": "+", "frame": 1, "gene": "tsf", "product": "translation elongation factor Ts", "db_xrefs": [ "COG:COG0264", "COG:J", "GO:0003746", "GO:0005737", "GO:0006414", "SO:0001217", "UniRef:UniRef50_P47246", "UniRef:UniRef90_P47246" ], "nt": "ATGGTTGAGATAAACTCACAAACTGATTTTGTAGCCAAAAATCAAGAGTTAAAAGAATTTTCAGATTTAATGCTTGAAAAAATATTTGAAAAAGTAAATCCAAAAACAGAATTAGTTGAAATTGAAAAAATTCAAATTAATAATGATGAAAAAGTTAGTGAAAAACTAGCATTAATTGCTTCTAAAACTGATGAGAAAATAGTACTTAGAAGAGTAGTTGTATTTGAAACTAAAACTAATCAAATTTTCACCTATTTACATGCCAATAAAAGAATTGGGGTAATTATTGAGATTCAAGGAAAACTCAACGAAGATGATGGTAAGCATTTAGCAATGCATATTGCTGCTAATTCACCACAATTTATTGATCAAAGTGATGTTAATCAAACATGA", "aa": "MVEINSQTDFVAKNQELKEFSDLMLEKIFEKVNPKTELVEIEKIQINNDEKVSEKLALIASKTDEKIVLRRVVVFETKTNQIFTYLHANKRIGVIIEIQGKLNEDDGKHLAMHIAANSPQFIDQSDVNQT", "aa_hexdigest": "c5609d87f049217d1d811b231ae7842f", "start_type": "ATG", "rbs_motif": null, "genes": [ "tsf" ], "truncated": "both", "pseudogene": { "start": 539119, "stop": 540015, "observations": { "mutation": [ 539233, 539722 ], "stop": [ 539233, 539722 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47246", "query_cov": 1.0, "subject_cov": 0.436241610738255, "identity": 1.0, "score": 244.0, "evalue": 1.81e-79, "gene_start": 1, "gene_end": 130, "reference_start": 72, "reference_end": 201, "reference_sequence": "MATKIELIKELRKSTQASVMDCKQALEKNNDDFEKAVKWLRENGIVKSTKKLNKVASEGIIVLKSNLHKAIMVEINSQTDFVAKNQELKEFSDLMLEKIFEKVNPKTELVEIEKIQINNDEKVSEKLALIASKTDEKIVLRRVVVFETKTNQIFTYLHANKRIGVIIEIQGKLNEDDGKHLAMHIAANSPQFIDQSDVNQTWLQNERNIIRSQAELEVKENPKKAIFLEKTIEGRVNKLLIDTCLINQKYLIDETKTIGQFLKEKQAKVLKFIRYEVGEGIIKETVDFVSEVNAQIKQ" }, "paralog": false, "identity": 0.9932885906040269, "score": 562.0, "evalue": 3.93e-207, "description": "Internal stop codon at 539233, 539722. Nonsense mutation around 539233, 539722.", "psc": { "uniref90_id": "UniRef90_P47246", "gene": "tsf", "product": "translation elongation factor Ts", "uniref50_id": "UniRef50_P47246", "cog_id": "COG0264", "cog_category": "J", "go_ids": [ "GO:0003746", "GO:0005737", "GO:0006414" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47246", "UniRef:UniRef50_P47246", "COG:COG0264", "COG:J", "GO:0003746", "GO:0005737", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_P47246", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47246" ], "product": "Elongation factor Ts" } }, "id": "JPCNHPFPBM_978", "locus": "GCA000292505_00978" }, { "type": "cds", "sequence": "contig_1", "start": 539996, "stop": 540727, "strand": "+", "frame": 2, "gene": "pyrH", "product": "UMP kinase", "db_xrefs": [ "COG:COG0528", "COG:F", "EC:2.7.4.22", "GO:0005524", "GO:0005737", "GO:0006221", "GO:0006225", "GO:0015949", "GO:0033862", "GO:0044210", "KEGG:K09903", "RefSeq:WP_014894698.1", "SO:0001217", "UniParc:UPI00027B408D", "UniRef:UniRef100_UPI00027B408D", "UniRef:UniRef50_P47672", "UniRef:UniRef90_P47672" ], "nt": "ATGCACAAATCAAACAATAAAATCCGCCAAAGAATAATCATTAAACTTAGTGGTGCTGGGCTAACCAAAGAAAATTTTCAACCCTTTTCTAATGATTTTTTTGAAACTATTATTAATCAATTAAAAGTTTTAAAAGAAAGCTATCAAGTAGGAATTGTTATTGGTGGGGGTAACATTATCAGAGGTAATAATTGCCAAGAATTTAACATTGCTGAATACCATGGTCATCAACTTGGTATTATAGCAACAGTAGTTAATGGCTATTTTTTAAAAGCAAAGTTAGATGCACATAATTTGAAAAGTGCTTTACTAAGTGCAATTAGTTGTCCTAGTTTAGCAGTGCAAATTCTTTCACAGCAAACTATTGATAAAGCTTTTGAAGAGAATGACTTTGTCATTTTTTCAGGTGGCACTGGTAATCCTTATTTTTCCACTGACACTGCATTAGCTTTAAGAGCAGTGCAAACAAAAGCAGTTGCTATTCTGATTGGAAAAAATGGTGTTGATGGTGTTTATACAGCTGATCCTAAAAAAGATAAAAATGCAACCTTTTTACCAACACTCAACTATGACCATGCCATTAAAAATGATTTGAAAATTATGGATATTACTGCTTTTACTATGTGTAAGGAAAATAATCTGAAAATAATTATTTTTAACATTAATGCTGAGAATGCATTATTAGATGCATTAAACAAAAAAGGTCGCTTTACTATAATTGAAAATAACTAA", "aa": "MHKSNNKIRQRIIIKLSGAGLTKENFQPFSNDFFETIINQLKVLKESYQVGIVIGGGNIIRGNNCQEFNIAEYHGHQLGIIATVVNGYFLKAKLDAHNLKSALLSAISCPSLAVQILSQQTIDKAFEENDFVIFSGGTGNPYFSTDTALALRAVQTKAVAILIGKNGVDGVYTADPKKDKNATFLPTLNYDHAIKNDLKIMDITAFTMCKENNLKIIIFNINAENALLDALNKKGRFTIIENN", "aa_hexdigest": "3f34813cf7a3f6f8a5d04ca87632770f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B408D", "ncbi_nrp_id": "WP_014894698.1", "uniref100_id": "UniRef100_UPI00027B408D", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B408D", "RefSeq:WP_014894698.1", "UniRef:UniRef100_UPI00027B408D" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B408D", "uniref90_id": "UniRef90_P47672" }, "psc": { "uniref90_id": "UniRef90_P47672", "gene": "pyrH", "product": "UMP kinase", "ec_ids": [ "2.7.4.22" ], "uniref50_id": "UniRef50_P47672", "cog_id": "COG0528", "cog_category": "F", "kegg_orthology_id": "K09903", "go_ids": [ "GO:0005524", "GO:0005737", "GO:0006221", "GO:0006225", "GO:0015949", "GO:0033862", "GO:0044210" ] }, "pscc": { "uniref50_id": "UniRef50_P47672", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47672" ], "product": "Uridylate kinase" }, "genes": [ "pyrH" ], "id": "JPCNHPFPBM_979", "locus": "GCA000292505_00979" }, { "type": "cds", "sequence": "contig_1", "start": 540850, "stop": 541278, "strand": "+", "frame": 1, "gene": "frr", "product": "ribosome recycling factor", "db_xrefs": [ "COG:COG0233", "COG:J", "GO:0005737", "GO:0006412", "GO:0006415", "GO:0008135", "GO:0043023", "SO:0001217", "UniRef:UniRef50_P47673", "UniRef:UniRef90_P47673" ], "nt": "TTGATTGAAAGTTATGGACAAAAAATGCCTTTAATATCTTTAGCTCAAGTGACTATTAATCCGCCAAGAGAAATAATCATAAAACCATTTGATCCTAAGAGTAATACTAATGCTATTTACAGTGAAATTCAGCGGGCAAACATTGGTGTTCAACCAGTTATTGATGGTGAAAAAATTCGTGTTAATTTTCCCCAAATTACTCAAGAAACTCGCTTAGAAAATATTAAGCACGTTAAAAAAATAATAGAGCAAATTTATCAAGAACTGAGGGTTGTAAGAAGAGATGCATTACAAATGATTAAAAAAGATAATCACAATGAGGATTTAGAAAACTCTTTAAAAGCTGAAATAGAAAAAATTAACAAAAATTATTCTAATCAATTAGAAGAGATTCAAAAAGACAAAGAAAAAGAATTGCTAACAATTTAA", "aa": "MIESYGQKMPLISLAQVTINPPREIIIKPFDPKSNTNAIYSEIQRANIGVQPVIDGEKIRVNFPQITQETRLENIKHVKKIIEQIYQELRVVRRDALQMIKKDNHNEDLENSLKAEIEKINKNYSNQLEEIQKDKEKELLTI", "aa_hexdigest": "1026a425a53f60a7b5e25902a53eb132", "start_type": "TTG", "rbs_motif": null, "genes": [ "frr" ], "truncated": "5-prime", "pseudogene": { "start": 540727, "stop": 541278, "observations": { "mutation": [ 540784 ], "stop": [ 540784 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47673", "query_cov": 1.0, "subject_cov": 0.7759562841530054, "identity": 0.993, "score": 264.0, "evalue": 1.68e-88, "gene_start": 1, "gene_end": 142, "reference_start": 42, "reference_end": 183, "reference_sequence": "MTKAHYIDFFKQAADKKIQWLKEELTKIRTGRPNPKIFDNLLIESYGQKMPLISLAQVTINPPREIIIKPFDPKSNTNAIYSEIQRANIGVQPVIDGEKIRVNFPQITQETRLENIKHVKKIIEQIYQELRVVRRDALQMIKKDNHNEDLENSLKAEIEKINKNYSNQLEEIQKDKEKELLTI" }, "paralog": false, "identity": 0.994535519125683, "score": 357.0, "evalue": 1.35e-127, "description": "Internal stop codon at 540784. Nonsense mutation around 540784.", "psc": { "uniref90_id": "UniRef90_P47673", "gene": "frr", "product": "ribosome recycling factor", "uniref50_id": "UniRef50_P47673", "cog_id": "COG0233", "cog_category": "J", "go_ids": [ "GO:0005737", "GO:0006412", "GO:0006415", "GO:0008135", "GO:0043023" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47673", "UniRef:UniRef50_P47673", "COG:COG0233", "COG:J", "GO:0005737", "GO:0006412", "GO:0006415", "GO:0008135", "GO:0043023" ] }, "pscc": { "uniref50_id": "UniRef50_P47673", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47673" ], "product": "Ribosome-recycling factor" } }, "id": "JPCNHPFPBM_980", "locus": "GCA000292505_00980" }, { "type": "cds", "sequence": "contig_1", "start": 541275, "stop": 541580, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGTGAACAACTCTCAAATCATCAGAGAACCAATTACCCCAACAATTAAAATAATGATTATGCTAAAGCTGAAACGAACAGCTTTAAAGTTTTCATTAATCTCAGTACTAATAACACTAGTAACACCACTTGCTTGTTCTACTTTTAATGTTCTTGAATTGAAATCTGCTGATCACGGTGATCAAAAGTTAAAACCATCAGCAAATGCGCTAATTAAAAGAAAAAAGCAAAATACAACTAATAAAGCAATGAATACTGAAGTTCGCTTTTTGATGAATTGTTTTGCTTTTTCATTCATTTAA", "aa": "MFVNNSQIIREPITPTIKIMIMLKLKRTALKFSLISVLITLVTPLACSTFNVLELKSADHGDQKLKPSANALIKRKKQNTTNKAMNTEVRFLMNCFAFSFI", "aa_hexdigest": "1f5a7a4795e76c8d92c36f3edb1f81c2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11444.656099999982, "isoelectric_point": 10.24945774078369 }, "id": "JPCNHPFPBM_981", "locus": "GCA000292505_00981" }, { "type": "cds", "sequence": "contig_1", "start": 541752, "stop": 541841, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAGCAATAAAGCAAGCAATAGCTCCTAAGCTATATGAAAGTTGTAAGCTAAACTTAATTCTTGTTTTACTCATGCAAGCAATGTAA", "aa": "MLAIKQAIAPKLYESCKLNLILVLLMQAM", "aa_hexdigest": "fa9b394f8ba216dce46217d0ad3627f1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3261.1224, "isoelectric_point": 9.102242851257323 }, "id": "JPCNHPFPBM_982", "locus": "GCA000292505_00982" }, { "type": "cds", "sequence": "contig_1", "start": 541851, "stop": 542021, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGCTAATGATGCCAAAAAAAGCTCCTTCTCATGTTTTTGATGGTGAAATTTTAATTAAAGGATTTTTACCAAAGCGCTTTCCAAAAAGAAAAGAAAATGTATCAACACTGACTGCTAAACTCATCAATAAAAAGATTGTAGTTCAACCCCTGATAACAGAAAAGTAA", "aa": "MVLMMPKKAPSHVFDGEILIKGFLPKRFPKRKENVSTLTAKLINKKIVVQPLITEK", "aa_hexdigest": "8633a74e7d51186d921461d7934546ed", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6402.811500000002, "isoelectric_point": 10.358860588073728 }, "id": "JPCNHPFPBM_983", "locus": "GCA000292505_00983" }, { "type": "cds", "sequence": "contig_1", "start": 542552, "stop": 543067, "strand": "+", "frame": 2, "gene": "hsdS", "product": "Putative type I specificity subunit S-MgeORF438P", "db_xrefs": [ "COG:COG0732", "COG:V", "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_Q49434", "UniRef:UniRef90_Q49434" ], "nt": "TTGTTTGATTTAAAAAAAGGTGAAATGCTTGAAAAAGAGTTAATTACACCTGAGGGAAAATATGAATATTTTAACGGTGGTGTAAAAAATTCAGGAAGAACTGACAAGTTCAATACTTTTAAGAACACTATTAGTGTAATTGTTGGTGGATCTTGTGGTTATGTAAGGCTAGCTGATAAAAATTTCTTTTGTGGTCAAAGTAATTGCACATTAAATTTGTTAGATCCGCTTGAACTTGATCTTAAATTTGCTTACTATGCTTTGAAATCACAACAAGAAAGAATAGAGGCTTTAGCTTTTGGGACCACGATTCAAAACATTAGAATTTCAGATTTAAAAGAATTGGAAATTCCTTTCACATCAAATAAGAATGAACAGCATGCTATTGCAAATACTTTAAGTGTTTTTGATGAGAGACTAGAAAACTTAGCTTCTTTAATTGAGATTAACAGGAAACTAAGAGATGAATATGCTCACAAACTCTTTAGCTTAGATGAAGCTTTTCTAAGTCATTGA", "aa": "MFDLKKGEMLEKELITPEGKYEYFNGGVKNSGRTDKFNTFKNTISVIVGGSCGYVRLADKNFFCGQSNCTLNLLDPLELDLKFAYYALKSQQERIEALAFGTTIQNIRISDLKELEIPFTSNKNEQHAIANTLSVFDERLENLASLIEINRKLRDEYAHKLFSLDEAFLSH", "aa_hexdigest": "e5fdeeacefa01ea5afc6057b9fd7a38f", "start_type": "TTG", "rbs_motif": null, "genes": [ "hsdS" ], "truncated": "both", "pseudogene": { "start": 542492, "stop": 543370, "observations": { "mutation": [ 542528, 543065 ], "stop": [ 542528, 543065 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49434", "query_cov": 1.0, "subject_cov": 0.4211822660098522, "identity": 0.9940000000000001, "score": 340.0, "evalue": 7.1e-115, "gene_start": 1, "gene_end": 171, "reference_start": 44, "reference_end": 214, "reference_sequence": "MGHHHHHHHHHHSSGHIDDDDKHMTPKLKLNNNINWTKRTIDSLFDLKKGEMLEKELITPEGKYEYFNGGVKNSGRTDKFNTFKNTISVIVGGSCGYVRLADKNFFCGQSNCTLNLLDPLELDLKFAYYALKSQQERIEALAFGTTIQNIRISDLKELEIPFTSNKNEQHAIANTLSVFDERLENLASLIEINRKLRDEYAHKLFSLDEAFLSHWKLEALQSQMHEITLGEIFNFKSGKYLKSEERLEEGKFPYYGAGIDNTGFVAEPNTEKDTISIISNGYSLGNIRYHEIPWFNGTGSIALEPMNNEIYVPFFYCALKYLQKDIKERMKSDDSPFLSLKLAGEIKVPYVKSFQLQRKAGKIVFLLDQKLDQYKKELSSLTVIRDTLLKKLFPDMTERTKSIKDY" }, "paralog": false, "identity": 0.9931506849315068, "score": 581.0, "evalue": 2.54e-212, "description": "Internal stop codon at 542528, 543065. Nonsense mutation around 542528, 543065.", "psc": { "uniref90_id": "UniRef90_Q49434", "gene": "HsdS", "product": "Putative type I specificity subunit S.MgeORF438P", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_Q49434", "cog_id": "COG0732", "cog_category": "V", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49434", "EC:3.1.21.3", "UniRef:UniRef50_Q49434", "COG:COG0732", "COG:V", "KEGG:K01154", "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_Q49434", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49434" ], "product": "Putative type I specificity subunit S.MgeORF438P" } }, "id": "JPCNHPFPBM_984", "locus": "GCA000292505_00984" }, { "type": "cds", "sequence": "contig_1", "start": 543092, "stop": 543643, "strand": "+", "frame": 2, "gene": "hsdS", "product": "Putative type I specificity subunit S-MgeORF438P", "db_xrefs": [ "COG:COG0732", "COG:V", "EC:3.1.21.3", "GO:0003677", "GO:0009307", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_Q49434", "UniRef:UniRef90_Q49434" ], "nt": "ATGCATGAAATTACTTTAGGGGAAATATTTAATTTCAAAAGTGGTAAATATCTAAAGAGCGAGGAGAGATTAGAAGAAGGGAAATTTCCTTATTATGGAGCGGGAATTGATAACACTGGTTTTGTAGCTGAACCTAATACTGAAAAAGACACAATTTCTATTATTTCTAATGGTTATTCACTAGGTAACATCAGGTATCATGAAATTCCTTGGTTTAATGGCACAGGTAGCATTGCATTAGAACCCATGAATAATGAAATCTATGTACCATTCTTTTACTGTGCTTTAAAGTATTTGCAAAAAGATATTAAAGAAAGAATGAAAAGTGATGATTCACCTTTTTTATCCTTAAAACTAGCAGGTGAAATTAAAGTGCCTTATGTTAAGTCATTTCAACTGCAAAGAAAGGCAGGAAAAATCGTCTTTTTGTTAGATCAAAAATTAGACCAATATAAAAAAGAACTAAGTTCTTTAACAGTGATTCGTGACACTTTGTTAAAAAAATTATTCCCTGATATGACTGAAAGAACTAAATCTATTAAGGATTATTAA", "aa": "MHEITLGEIFNFKSGKYLKSEERLEEGKFPYYGAGIDNTGFVAEPNTEKDTISIISNGYSLGNIRYHEIPWFNGTGSIALEPMNNEIYVPFFYCALKYLQKDIKERMKSDDSPFLSLKLAGEIKVPYVKSFQLQRKAGKIVFLLDQKLDQYKKELSSLTVIRDTLLKKLFPDMTERTKSIKDY", "aa_hexdigest": "7d31abd2f905c0cba3c8bd336bca395d", "start_type": "ATG", "rbs_motif": null, "genes": [ "hsdS" ], "truncated": "5-prime", "pseudogene": { "start": 542792, "stop": 543643, "observations": { "mutation": [ 543065 ], "stop": [ 543065 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49434", "query_cov": 1.0, "subject_cov": 0.45073891625615764, "identity": 1.0, "score": 368.0, "evalue": 9.67e-126, "gene_start": 1, "gene_end": 183, "reference_start": 224, "reference_end": 406, "reference_sequence": "MGHHHHHHHHHHSSGHIDDDDKHMTPKLKLNNNINWTKRTIDSLFDLKKGEMLEKELITPEGKYEYFNGGVKNSGRTDKFNTFKNTISVIVGGSCGYVRLADKNFFCGQSNCTLNLLDPLELDLKFAYYALKSQQERIEALAFGTTIQNIRISDLKELEIPFTSNKNEQHAIANTLSVFDERLENLASLIEINRKLRDEYAHKLFSLDEAFLSHWKLEALQSQMHEITLGEIFNFKSGKYLKSEERLEEGKFPYYGAGIDNTGFVAEPNTEKDTISIISNGYSLGNIRYHEIPWFNGTGSIALEPMNNEIYVPFFYCALKYLQKDIKERMKSDDSPFLSLKLAGEIKVPYVKSFQLQRKAGKIVFLLDQKLDQYKKELSSLTVIRDTLLKKLFPDMTERTKSIKDY" }, "paralog": false, "identity": 0.9964664310954063, "score": 556.0, "evalue": 2.22e-202, "description": "Internal stop codon at 543065. Nonsense mutation around 543065.", "psc": { "uniref90_id": "UniRef90_Q49434", "gene": "HsdS", "product": "Putative type I specificity subunit S.MgeORF438P", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_Q49434", "cog_id": "COG0732", "cog_category": "V", "kegg_orthology_id": "K01154", "go_ids": [ "GO:0003677", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49434", "EC:3.1.21.3", "UniRef:UniRef50_Q49434", "COG:COG0732", "COG:V", "KEGG:K01154", "GO:0003677", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_Q49434", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49434" ], "product": "Putative type I specificity subunit S.MgeORF438P" } }, "id": "JPCNHPFPBM_985", "locus": "GCA000292505_00985" }, { "type": "cds", "sequence": "contig_1", "start": 544048, "stop": 544401, "strand": "-", "frame": 1, "gene": null, "product": "putative lipoprotein MG439", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75150", "UniRef:UniRef90_P47677" ], "nt": "ATGACTGGTATAGTTGGTAGTGACAATACTAGTACTTCATTAATCAAATCCTTTTTAGCTAGTACAAGTGGTGTAAGTGAAAGTGATTTTACTGGTGATAAAGCTAATTTTGATGGTGATGTTATCTTTACTTATACTCCTCCAACAGATAATAGGAGGGTAAGTGAAAGTACTTTTAGTAGTATACCTGCTTCAATTAACTTTCCTTTTGATATCAAAATTGACATGAGTACTTCTCATGAAAAGTTGAATTTATTACTTTCAACAAATGAACAAGTGAAGAAAATTAGAACAAGAACTTTTAAGGGAAAGTCAATCGATCTTTTACCGTTTTTTTATTACACATTGCTTTAA", "aa": "MTGIVGSDNTSTSLIKSFLASTSGVSESDFTGDKANFDGDVIFTYTPPTDNRRVSESTFSSIPASINFPFDIKIDMSTSHEKLNLLLSTNEQVKKIRTRTFKGKSIDLLPFFYYTLL", "aa_hexdigest": "a03fffb2e085b6b3d2d4fdce297da6b4", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 544048, "stop": 544701, "observations": { "mutation": [ 544443, 544659 ], "stop": [ 544443, 544659 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47677", "query_cov": 1.0, "subject_cov": 0.43014705882352944, "identity": 1.0, "score": 226.0, "evalue": 6.37e-73, "gene_start": 1, "gene_end": 117, "reference_start": 156, "reference_end": 272, "reference_sequence": "MKLRKIFLLPLISLSTLSVACSSTDPGLEKAQAYFQRNNIELSKNNAVTFLKKGYSSDKEEVINTVFASWKTTLLDYQLLEKPLDYSRFAKAFGVNKSKEDVTPNISAKGLYFDETYPGISGQIALVLGVKSQKVVNFQYSWKNNLDFKVQIHLKMTGIVGSDNTSTSLIKSFLASTSGVSESDFTGDKANFDGDVIFTYTPPTDNRRVSESTFSSIPASINFPFDIKIDMSTSHEKLNLLLSTNEQVKKIRTRTFKGKSIDLLPFFYYTLL" }, "paralog": false, "identity": 0.9907834101382489, "score": 419.0, "evalue": 2.87e-151, "description": "Internal stop codon at 544443, 544659. Nonsense mutation around 544443, 544659.", "psc": { "uniref90_id": "UniRef90_P47677", "product": "Uncharacterized lipoprotein MG439", "uniref50_id": "UniRef50_P75150", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47677", "UniRef:UniRef50_P75150", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75150", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75150" ], "product": "Uncharacterized lipoprotein MG439 homolog 1" } }, "id": "JPCNHPFPBM_986", "locus": "GCA000292505_00986" }, { "type": "cds", "sequence": "contig_1", "start": 544657, "stop": 544866, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGAAACTCAGAAAGATCTTTTTATTACCATTAATTTCACTTAGTACTTTATCTGTAGCCTGTTCATCAACTGATCCTGGTTTAGAAAAAGCACAAGCTTATTTTCAGAGAAACAATATAGAACTTTCAAAAAATAATGCTGTTACTTTTTTAAAAAAGGGTTATAGTTCTGATAAGGAAGAGGTAATTAATACTGTATTTGCCAGTTGA", "aa": "MKLRKIFLLPLISLSTLSVACSSTDPGLEKAQAYFQRNNIELSKNNAVTFLKKGYSSDKEEVINTVFAS", "aa_hexdigest": "a6b45fd7970a198ce4bbfbb90c70e952", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 39, "aa_cov": 0.5507246376811594, "hmm_cov": 1.0, "evalue": 5.4091695269067064e-09, "score": 30.970706939697266, "start": 32, "stop": 69 } ], "seq_stats": { "molecular_weight": 7669.7820999999985, "isoelectric_point": 9.25103588104248 }, "id": "JPCNHPFPBM_987", "locus": "GCA000292505_00987" }, { "type": "cds", "sequence": "contig_1", "start": 544866, "stop": 545438, "strand": "-", "frame": 2, "gene": null, "product": "putative protein MG440", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47678", "UniRef:UniRef90_P47678" ], "nt": "TTGCAAGACGAAAAGATTCTTGAAAAACAAGACCCAAGTAGATTTGTAAAAGCATTTGGCAGTGGTAAGAGTAAGGAAGATGTTGAACCAAGCACTGGTGTTAAAGGCTTACGTTTAGTTGAAAGATACACGCAAAATGTTGCAAGTATTATTAATAATGTAATTAAATTGAGTAGTCAAACAGTTACAGATTTTAGTTTTTACTATAACAGTTCTCGTGATTTCAAAGTCCAAATAAGGTTACAAGCTACTGGAACTTTTGACAGTGCTCAAGCAAAATCATATTTGAGTCAAATAGGATTAAGTGATAGTGATATAAAAGATAAAAATTCTATTTCAGCTGATTTAATTTTTACTTATACTCCACCTTCCAGTAATTTATTTTCCAAATCTAGTTTTGATACTCTATTGAGAAAAATTAATTTTAATACCAATTTAAGGTTACAAATTATTGGTAAAGATGAATTGATGCAAAAGTTATTACAAAGTAATTTTGTAGGTCAATTAGCTGATCAAAACTTCCAAGATCAAACTATCGATCTTTTACCATATGTGCTTTATTCCATTTTGTAA", "aa": "MQDEKILEKQDPSRFVKAFGSGKSKEDVEPSTGVKGLRLVERYTQNVASIINNVIKLSSQTVTDFSFYYNSSRDFKVQIRLQATGTFDSAQAKSYLSQIGLSDSDIKDKNSISADLIFTYTPPSSNLFSKSSFDTLLRKINFNTNLRLQIIGKDELMQKLLQSNFVGQLADQNFQDQTIDLLPYVLYSIL", "aa_hexdigest": "aa48a3e9d5860d5cfb0b2536a55cf1cc", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 544866, "stop": 545690, "observations": { "mutation": [ 545450, 545672 ], "stop": [ 545450, 545672 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47678", "query_cov": 1.0, "subject_cov": 0.6934306569343066, "identity": 0.995, "score": 359.0, "evalue": 4.59e-124, "gene_start": 1, "gene_end": 190, "reference_start": 85, "reference_end": 274, "reference_sequence": "MLKKQFWTKAGIISSLMVLGSLITACSNLSSGTNFFSLNQLRNSFSKNSELNQDKKELVTSLRDSFEVDSKSATNVLLDAWRFSLQDEKILEKQDPSRFVKAFGSGKSKEDVEPSTGVKGLRLVERYTQNVASIINNVIKLSSQTVTDFSFYYNSSRDFKVQIRLQATGTFDSAQAKSYLSQIGLSDSDIKDKNSISADLIFTYTPPSSNLFSKSSFDTLLRKINFNTNLRLQIIGKDELMQKLLQSNFVGQLADQNFQDQTIDLLPYVLYSIL" }, "paralog": false, "identity": 0.9890510948905109, "score": 514.0, "evalue": 1.85e-187, "description": "Internal stop codon at 545450, 545672. Nonsense mutation around 545450, 545672.", "psc": { "uniref90_id": "UniRef90_P47678", "product": "Uncharacterized protein MG440", "uniref50_id": "UniRef50_P47678", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47678", "UniRef:UniRef50_P47678" ] }, "pscc": { "uniref50_id": "UniRef50_P47678", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47678" ], "product": "Uncharacterized protein MG440" } }, "id": "JPCNHPFPBM_988", "locus": "GCA000292505_00988" }, { "type": "cds", "sequence": "contig_1", "start": 545448, "stop": 545642, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00938.23" ], "nt": "ATGGTATTAGGTTCTTTAATTACAGCATGCTCTAACTTAAGCAGTGGAACAAATTTTTCCAGTTTAAACCAACTTAGAAATAGTTTCAGTAAAAATAGTGAATTAAATCAAGACAAGAAAGAGTTAGTTACTTCACTAAGAGATAGTTTTGAAGTGGATTCAAAAAGTGCTACAAACGTTCTATTAGATGCTTGA", "aa": "MVLGSLITACSNLSSGTNFSSLNQLRNSFSKNSELNQDKKELVTSLRDSFEVDSKSATNVLLDA", "aa_hexdigest": "11ffeae36c3bc70ac7bf65898d4e2301", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00938.23", "name": "Lipoprotein_3", "length": 39, "aa_cov": 0.609375, "hmm_cov": 1.0, "evalue": 9.89526984136832e-11, "score": 36.658443450927734, "start": 26, "stop": 64 } ], "seq_stats": { "molecular_weight": 6926.618800000002, "isoelectric_point": 5.042550849914551 }, "id": "JPCNHPFPBM_989", "locus": "GCA000292505_00989" }, { "type": "cds", "sequence": "contig_1", "start": 546365, "stop": 547177, "strand": "-", "frame": 3, "gene": "ylqF", "product": "ribosome biogenesis GTPase YlqF", "db_xrefs": [ "COG:COG1161", "COG:J", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0042254", "SO:0001217", "UniRef:UniRef50_P75135", "UniRef:UniRef90_Q49435" ], "nt": "ATGGACACATATACCTCCGCCAAAATTAATTGGTTCCCGGGTCACATGAAAAAGGCCCATGATCAATTAAAAAAGTTATCTAGTCAAATTGATGGCATCATTGAGATAGTAGATGCTAGAGCACCAACTTTAACTCATAATTCAGAAATAATTAGTTATTTTTTAAATAAACCAAAATTAATACTGGCATTAAAGACAGATTTAGCACAATACAAACCAAATAAAAAGATCTTATTTGGTTCATTAAAAGAACCTTTTAAACTCAAAAAAAAAGTTCTAAAGACATTAACAACTTTATTTGCAAATAAAAGACAACAATTAAAAGCAAAAGGCTTATTAATCAAACAATTTAGATTAGCAGTTATTGGCATGCCCAATGTAGGTAAATCTAGTTTAATTAACCTTTTGATAAATAAAAATCACTTAAAAGTAGCTAACCGCGCTGGTATTACTAAATCATTAAATTGGATTCAAATTAGCCCTGAATTATTATTAAGTGATACCCCAGGAATTTTTTTGAAAAGAATTGATGAAATTCAAATTGGATATAAACTAGTTTTGACTAATGTTATTAGAAGAGAAGTAGTTAATATAGAAGAAGTGGGAATGTTTGCTTTTAATTATCTTAAGAAACATTACAAACAGCTTTTACCATTTGAAGCGGATAGCTTTATTAACTTTTTGGAAAAATTTGCTAAAGTGAGAGGCTTAATTAAGAAAGCTAATGAACTAAACACTAACTTAGCATGTGAAATTTTTATTAATGAACTTATTAATGGGAAATATGGCAAATTAAGTTATGAACTTAATTAG", "aa": "MDTYTSAKINWFPGHMKKAHDQLKKLSSQIDGIIEIVDARAPTLTHNSEIISYFLNKPKLILALKTDLAQYKPNKKILFGSLKEPFKLKKKVLKTLTTLFANKRQQLKAKGLLIKQFRLAVIGMPNVGKSSLINLLINKNHLKVANRAGITKSLNWIQISPELLLSDTPGIFLKRIDEIQIGYKLVLTNVIRREVVNIEEVGMFAFNYLKKHYKQLLPFEADSFINFLEKFAKVRGLIKKANELNTNLACEIFINELINGKYGKLSYELN", "aa_hexdigest": "0479ebf250f7f800243cbcafd18ba8c6", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q49435", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.993, "score": 520.0, "evalue": 2.95e-186, "valid": true, "gene": "ylqF", "product": "ribosome biogenesis GTPase YlqF", "uniref50_id": "UniRef50_P75135", "cog_id": "COG1161", "cog_category": "J", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006412", "GO:0042254" ] }, "pscc": { "uniref50_id": "UniRef50_P75135", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75135" ], "product": "Probable ribosome biogenesis GTPase A" }, "genes": [ "ylqF" ], "id": "JPCNHPFPBM_990", "locus": "GCA000292505_00990" }, { "type": "cds", "sequence": "contig_1", "start": 547149, "stop": 547544, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF10035.14" ], "nt": "TTGTTGCAAGATGTCAATAATTATCGTGATTCTGCTTGGGAAGTTAGTTTATTTTTCTCACCTAATTTAATTGCAACTTTTTTTTCAATTTTGTTAACAGGAACAGTAGTTAGTTATCTTTTCCCTCGTTATAATTTTGCTGAAATTAAAGTATTTACTGATAAGCTTGAAGAAGTTAGAAAAGCATTGTTAAGTGATAATGCTAATCACAGTTTATCTATTCAAGAAACGCTTGGTGGTTATTCTTTACTCAAAAAGAAGATGATAGTTTCTGTTTCCATGTATGTAGAAATTCCCCATTTAATTAAGATTATTAGACAAATTGACAAGGATTGTTTGGTATCTATTACAAGAATCAGGGGAATAGATGGACACATATACCTCCGCCAAAATTAA", "aa": "MLQDVNNYRDSAWEVSLFFSPNLIATFFSILLTGTVVSYLFPRYNFAEIKVFTDKLEEVRKALLSDNANHSLSIQETLGGYSLLKKKMIVSVSMYVEIPHLIKIIRQIDKDCLVSITRIRGIDGHIYLRQN", "aa_hexdigest": "f452ed3ff956d850cb1ace51fa8b00ee", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10035.14", "name": "DUF2179", "length": 47, "aa_cov": 0.35877862595419846, "hmm_cov": 1.0, "evalue": 5.272452643417674e-10, "score": 34.167503356933594, "start": 78, "stop": 124 } ], "seq_stats": { "molecular_weight": 15101.419499999987, "isoelectric_point": 8.7098237991333 }, "id": "JPCNHPFPBM_991", "locus": "GCA000292505_00991" }, { "type": "cds", "sequence": "contig_1", "start": 547839, "stop": 548336, "strand": "-", "frame": 2, "gene": "yitT", "product": "putative protein MG443", "db_xrefs": [ "COG:COG1284", "COG:S", "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47681", "UniRef:UniRef90_P47681" ], "nt": "ATGAAATTTTTTAACAACTTATTTAAAAAAGAATCAAAGATAACAGTCACATCTGGTTCTAAACGCGTAAGGATTTCTAACTCTTTTTTGATGTTTAGCAATCTTTATGAGGCTAAAAAACCATTAAAGTATGCATTAGTTTACTTACTATCAATTATTAATGCTTTTCTTTTACTTATTTTTATTCAAAAAACAGGTTTATATAGCTTTGGAATTAGTTCTTTAACTCAAGGTTTTGCTAGATTAGTGTTTGTTTTGTTAAAAAGTTTTGATGAAACTCAAAGATTATTAATTTTTAACATCCTTTACTGGCTTTTATATGTCTTTATCAACATCCCTTTAATTATTTTTTCTTATAAAAAAATAGGTAAAAACTTTACTATTCTTTCAACCCATTTTGTTGTTGCATCTAACGTATTTGGCTTTTTAATCAGTATTATTCCAGGTTCTGATAATTTACCACCAATGTTAGCTTCTATAACAGATACTAACTTTTGA", "aa": "MKFFNNLFKKESKITVTSGSKRVRISNSFLMFSNLYEAKKPLKYALVYLLSIINAFLLLIFIQKTGLYSFGISSLTQGFARLVFVLLKSFDETQRLLIFNILYWLLYVFINIPLIIFSYKKIGKNFTILSTHFVVASNVFGFLISIIPGSDNLPPMLASITDTNF", "aa_hexdigest": "e1965d5955b3cb738e260aeeb5638285", "start_type": "ATG", "rbs_motif": null, "genes": [ "yitT" ], "truncated": "5-prime", "pseudogene": { "start": 547536, "stop": 548336, "observations": { "mutation": [ 547790, 547841 ], "stop": [ 547790, 547841 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47681", "query_cov": 1.0, "subject_cov": 0.4177215189873418, "identity": 0.988, "score": 306.0, "evalue": 1.22e-101, "gene_start": 1, "gene_end": 165, "reference_start": 1, "reference_end": 165, "reference_sequence": "MKFFNNLFKKESKITVASGSKRVRISNSFLMFSNLYEAKKPLKYVLVYLLSIINAFLLLIFIQKTGLYSFGISSLTQGFARLVFVLLKSFDETQRLLIFNILYWLLYVFINIPLIIFSYKKIGKNFTILSTHFVVASNVFGFLISIIPGSDNLPPMLASITDTNFWKAAKDLNQSAGFVPFLWSDTSQGNVIISTFIYAAIYGFYNGISVSLLYILGGSAGGADFLTQYYARKKNRSVGSILFYVNSFILIIAILIGSFVAGSLLLQDVNNYRDSAWEVSLFFSPNLIATFFSILLTGTVVSYLFPRYNFAEIKVFTDKLEEVRKALLSDNANHSLSIQETLGGYSLLKKKMIVSVSMYVEIPHLIKIIRQIDKDCLVSITRIRGIDGHIYLRQN" }, "paralog": false, "identity": 0.9849624060150376, "score": 501.0, "evalue": 4.15e-181, "description": "Internal stop codon at 547790, 547841. Nonsense mutation around 547790, 547841.", "psc": { "uniref90_id": "UniRef90_P47681", "gene": "YitT", "product": "Uncharacterized protein MG443", "uniref50_id": "UniRef50_P47681", "cog_id": "COG1284", "cog_category": "S", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47681", "UniRef:UniRef50_P47681", "COG:COG1284", "COG:S", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47681", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47681" ], "product": "Uncharacterized protein MG443" } }, "id": "JPCNHPFPBM_992", "locus": "GCA000292505_00992" }, { "type": "cds", "sequence": "contig_1", "start": 548352, "stop": 548711, "strand": "-", "frame": 2, "gene": "rplS", "product": "50S ribosomal protein L19", "db_xrefs": [ "COG:COG0335", "COG:J", "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02884", "RefSeq:WP_010869484.1", "SO:0001217", "UniParc:UPI0000133CB2", "UniRef:UniRef100_P47682", "UniRef:UniRef50_P47682", "UniRef:UniRef90_P47682" ], "nt": "ATGAAAAAAATAAATAAACAAGCATTAATTGATGCAGTAGAACAAAAACAGTTAAAGGAATATGTTCCTGAATTTGGAGCAGGAGATGAAGTTAATGTTGCTATTAAGTTACGTGAAAAAGAAAAAGTTCGAGTTCAAAACTTCACTGGAACTGTTTTAAGAAGAAGGGGAAGAGGGATTAGCGAAACTTTCATGGTAAGAAAAACCACTGATGGAATTCCTATTGAAAAAAACTTTCAAATCCACAACCCTAATATAGACATAGAAGTAAAACGCAGGGGTAAAGTAAGAAGAGCATACATCTCTTATATGCGTGAAAGATCTGGTAAGTCAGCAAAAATTAAAGAAAAAAAGTCTTAA", "aa": "MKKINKQALIDAVEQKQLKEYVPEFGAGDEVNVAIKLREKEKVRVQNFTGTVLRRRGRGISETFMVRKTTDGIPIEKNFQIHNPNIDIEVKRRGKVRRAYISYMRERSGKSAKIKEKKS", "aa_hexdigest": "69ed898e384468424d405a0c31417fa3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133CB2", "ncbi_nrp_id": "WP_010869484.1", "uniref100_id": "UniRef100_P47682", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133CB2", "RefSeq:WP_010869484.1", "UniRef:UniRef100_P47682" ] }, "ips": { "uniref100_id": "UniRef100_P47682", "uniref90_id": "UniRef90_P47682" }, "psc": { "uniref90_id": "UniRef90_P47682", "gene": "rplS", "product": "50S ribosomal protein L19", "uniref50_id": "UniRef50_P47682", "cog_id": "COG0335", "cog_category": "J", "kegg_orthology_id": "K02884", "go_ids": [ "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_P47682", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47682" ], "product": "Large ribosomal subunit protein bL19" }, "genes": [ "rplS" ], "id": "JPCNHPFPBM_993", "locus": "GCA000292505_00993" }, { "type": "cds", "sequence": "contig_1", "start": 548695, "stop": 549237, "strand": "-", "frame": 1, "gene": null, "product": "tRNA (guanine-N(1)-)-methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B1GZ20" ], "nt": "ATGGCTTATGGTGGAGGAAGTGGCATGGTTTTAAAAGCTGAACCTATTATTAATTGTTTAAATTTTTATAAAGCCCCAAATTCTCATGTAGTTTTACTCTCCCCAGAAGGTGAACAATTTTCTCAGAATTGTGCTAAAAAACTTACAAAATACGAACACTTAATTTTGTTATCTGGTCACTATGAAGGTTTTGATCAAAGAATTTATAAATATATTAATCAAATTGTTTCTTTAGGTGATTTTGTTTTAAGTGGTGGGGAACTTGTAGCACTAAGTGTTATTGATGCTACTGTTAGATTAATTAAAGGAGTTATTAATGATCAGAGTCTTATTTGTGAATCATTCAATGATAATCTATTAGATTTTCCTGTTTATACAAGGCCATACGATTTAAAAGGCGATAAAGTTCCTGAAGTTTTACTTTCAGGAGATCACCAAAAGATTGAATCATTTCGTAAAGAACAGCAAATCTTAAAAACTGCAAAATACAGACCTGATTTATACAAAAAATACTTAGAAAATAAAAATGAAAAAAATAAATAA", "aa": "MAYGGGSGMVLKAEPIINCLNFYKAPNSHVVLLSPEGEQFSQNCAKKLTKYEHLILLSGHYEGFDQRIYKYINQIVSLGDFVLSGGELVALSVIDATVRLIKGVINDQSLICESFNDNLLDFPVYTRPYDLKGDKVPEVLLSGDHQKIESFRKEQQILKTAKYRPDLYKKYLENKNEKNK", "aa_hexdigest": "388a0d535101325537d8b8d092f8f76d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A534NV99", "query_cov": 0.9444444444444444, "subject_cov": 0.821256038647343, "identity": 0.506, "score": 164.0, "evalue": 5.18e-48, "valid": false, "gene": "trmD", "product": "tRNA (guanosine(37)-N1)-methyltransferase TrmD", "ec_ids": [ "2.1.1.228" ], "uniref50_id": "UniRef50_B1GZ20", "kegg_orthology_id": "K00554", "go_ids": [ "GO:0008033", "GO:0052906" ] }, "pscc": { "uniref50_id": "UniRef50_B1GZ20", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B1GZ20" ], "product": "tRNA (guanine-N(1)-)-methyltransferase" }, "genes": [], "id": "JPCNHPFPBM_994", "locus": "GCA000292505_00994" }, { "type": "cds", "sequence": "contig_1", "start": 549277, "stop": 549390, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAATCACTGTTTTAACACTTTTTGAAAACACTATTTGGCCTTACTTAAATAGTTCTATTATGTTACAAGCTCAAAAAGCAAATTTAGTTCAATTTGAAGTAGTAAATTGA", "aa": "MKITVLTLFENTIWPYLNSSIMLQAQKANLVQFEVVN", "aa_hexdigest": "1cc73688319b6ba21c945286dd1b2383", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4298.030700000001, "isoelectric_point": 5.897352409362793 }, "id": "JPCNHPFPBM_995", "locus": "GCA000292505_00995" }, { "type": "cds", "sequence": "contig_1", "start": 549504, "stop": 549659, "strand": "-", "frame": 2, "gene": "rpsP", "product": "30S ribosomal protein S16", "db_xrefs": [ "COG:COG0228", "COG:J", "GO:0000314", "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0022627", "SO:0001217", "UniRef:UniRef50_P47684", "UniRef:UniRef90_P47684" ], "nt": "ATGCGGATGGGAAGAGTACACTATCCGCTTTATAGAATAGTAGCGGTTGATTCGCGAGTAAAGCGTAATGGAAAGTATATCGCTTTAATTGGACATCTAAATCCAGCTTTAAAGGAAAATAAGTGTAAATTAGATGAAACTGTTGCCTTAGATTGA", "aa": "MRMGRVHYPLYRIVAVDSRVKRNGKYIALIGHLNPALKENKCKLDETVALD", "aa_hexdigest": "b576cd2be2db58e53cd9ebf649b721c7", "start_type": "ATG", "rbs_motif": null, "genes": [ "rpsP" ], "truncated": "5-prime", "pseudogene": { "start": 549390, "stop": 549677, "observations": { "mutation": [ 549506 ], "stop": [ 549506 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47684", "query_cov": 1.0, "subject_cov": 0.5368421052631579, "identity": 1.0, "score": 106.0, "evalue": 7.08e-29, "gene_start": 1, "gene_end": 51, "reference_start": 7, "reference_end": 57, "reference_sequence": "MVKIRLMRMGRVHYPLYRIVAVDSRVKRNGKYIALIGHLNPALKENKCKLDETVALDWLNKGAIPTDTVRSLFSESGLWKKFIESKNKKETSPKK" }, "paralog": false, "identity": 0.9789473684210527, "score": 187.0, "evalue": 1.85e-63, "description": "Internal stop codon at 549506. Nonsense mutation around 549506.", "psc": { "uniref90_id": "UniRef90_P47684", "gene": "rpsP", "product": "30S ribosomal protein S16", "uniref50_id": "UniRef50_P47684", "cog_id": "COG0228", "cog_category": "J", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0022627" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47684", "UniRef:UniRef50_P47684", "COG:COG0228", "COG:J", "GO:0000314", "GO:0003735", "GO:0005737", "GO:0006412", "GO:0015935", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_P47684", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47684" ], "product": "Small ribosomal subunit protein bS16" } }, "id": "JPCNHPFPBM_996", "locus": "GCA000292505_00996" }, { "type": "cds", "sequence": "contig_1", "start": 550287, "stop": 550424, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAACTGCAAATGCTGCCTTAGCAGGTTTAATTAATATTGCTTTTGGTCAAATTCACACTAATCTGTTTATGGATAAATATTTCGCTAATCCACCACAAAATAAGTTGGTCAAATTTATTGAAAAGTACTCATAA", "aa": "MLTANAALAGLINIAFGQIHTNLFMDKYFANPPQNKLVKFIEKYS", "aa_hexdigest": "9c40812b28e08467067f1c353f298c22", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5057.8851, "isoelectric_point": 9.30383548736572 }, "id": "JPCNHPFPBM_997", "locus": "GCA000292505_00997" }, { "type": "cds", "sequence": "contig_1", "start": 550524, "stop": 550703, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002F172CE", "UniRef:UniRef90_UPI0002F172CE" ], "nt": "TTGTTAACAGCAGTTGCTTTTGGAAAACAGATCTTATCTAGTTTTTTTGATGTTTCAGATCAAAATTTGGATTTAGCAAATTACTTTTCATTAATAGTGCAAGCACAAGTTTTCTTTGTAGCCACAGGCGCTAATAGTCAACAATATTTTCAAAATACTAACAGAGTGCTTTATTCTTGA", "aa": "MLTAVAFGKQILSSFFDVSDQNLDLANYFSLIVQAQVFFVATGANSQQYFQNTNRVLYS", "aa_hexdigest": "576de6bc10b5fdc83b8205bb29af8187", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 550287, "stop": 550703, "observations": { "mutation": [ 550427, 550526 ], "stop": [ 550427, 550526 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0002F172CE", "query_cov": 1.0, "subject_cov": 0.427536231884058, "identity": 1.0, "score": 116.0, "evalue": 3.81e-32, "gene_start": 1, "gene_end": 59, "reference_start": 1, "reference_end": 59, "reference_sequence": "MLTAVAFGKQILSSFFDVSDQNLDLANYFSLIVQAQVFFVATGANSQQYFQNTNRVLYSWIVSLTHGLFVFIPLLFIFQAITLQTNNIEVFIWLLTANAALAGLINIAFGQIHTNLFMDKYFANPPQNKLVKFIEKYS" }, "paralog": false, "identity": 0.9782608695652174, "score": 261.0, "evalue": 4.88e-92, "description": "Internal stop codon at 550427, 550526. Nonsense mutation around 550427, 550526.", "psc": { "uniref90_id": "UniRef90_UPI0002F172CE", "uniref50_id": "UniRef50_UPI0002F172CE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0002F172CE", "UniRef:UniRef50_UPI0002F172CE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0002F172CE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002F172CE" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6632.377400000003, "isoelectric_point": 4.427894020080567 }, "id": "JPCNHPFPBM_998", "locus": "GCA000292505_00998" }, { "type": "cds", "sequence": "contig_1", "start": 550815, "stop": 551453, "strand": "-", "frame": 2, "gene": null, "product": "MATE family efflux transporter", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FCE1E4D", "UniRef:UniRef90_UPI002FCE1E4D" ], "nt": "TTGGTTTTATCTTTTGCAAAAGAATGGTTGCATTATAATCTTGATCAATCAAGTTTTGAACAAAATTTTCAAGCTAATAGCTTCCAACAGTTTTTTAACAAAAAGGCTATTGATGTAGCATCTGAATATGTTTATATTTTGATTGGTCTTAACATCATTCCTATGCTTTCAAGGTTGTTTTTTTATTTAGCACAATCAGAGGGAAGACAACTTTTTATTGCAATTGTTCCGCCTATTGCTAATTTGATTAACATATTAATAGTTTTTTTATTAGTTAGATATTCTTCTTTAGGAGTAATAGGATCAGCTGTGGCTGGAATACTAGGATACTTAATTAATTTTTTAGCTTATATTATTTATCTAATTCATTTAAACAAAAGAAATCTTACTTATTTAACTTTTAAAACTATTAAATTAAACAAAATTGACTTTAATCTACTAGTTGTAGTTTCATTAATAGGAATAGCATCCTTTTTTAGAAATGGATCACTTTCTATTGTTACTACATTTTATGAGAGTTTTTTGGTTAATTTGACAAAAGCAACTACTGATAAAAATGATGTTTTTTATCTGACACTTTTAACTGGACCTATTGCTATTAGTAATTTAGCTTCAGCAGCTATTTTTGGTTTATTATAA", "aa": "MVLSFAKEWLHYNLDQSSFEQNFQANSFQQFFNKKAIDVASEYVYILIGLNIIPMLSRLFFYLAQSEGRQLFIAIVPPIANLINILIVFLLVRYSSLGVIGSAVAGILGYLINFLAYIIYLIHLNKRNLTYLTFKTIKLNKIDFNLLVVVSLIGIASFFRNGSLSIVTTFYESFLVNLTKATTDKNDVFYLTLLTGPIAISNLASAAIFGLL", "aa_hexdigest": "5f85522426bb22d5195e309af490c042", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 550709, "stop": 551753, "observations": { "insertion": [ 550778 ], "mutation": [ 550817, 551504 ], "stop": [ 550817, 551504 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FCE1E4D", "query_cov": 1.0, "subject_cov": 0.5221674876847291, "identity": 0.991, "score": 316.0, "evalue": 9.23e-105, "gene_start": 1, "gene_end": 212, "reference_start": 160, "reference_end": 371, "reference_sequence": "MNSKRDRFEKQLLIKNVFESKQLFLTILRFTVPTFFFALFSAAYVFIDQIMVIKFVPRSELNPDSIFTDQSLIDEFKNSAFYKSDSFLSSGLNIKQFIKTVLNISQPLIILLNAINIFIPLGTGVIFSKAIGRNDQNKIQEAWNTGLISTTVFGLITQFLVLSFAKEWLHYNLDQSSFEQNFQANSFQQFFNKKAIDVASEYVYILIGLNIIPMLSRLFFYLAQSEGRQLFIAIVPPIANLINILIVFLLVRYSSLGVIGSAVAGILGYLINFLAYIIYLIYLNKRNLTYLTFKTIKLNKIDFNLLVVVSLIGMASFFRNGSLSIVTTFYESFLVNLTKATTDKNDVFYLTLLTGPIAISNLASAAIFGLLQGVRTVSSYKFGQKNMMKLKKLTFIQLLSVYRSVV" }, "paralog": false, "identity": 0.9884726224783862, "score": 573.0, "evalue": 1.97e-208, "description": "Internal stop codon at 550817, 551504. Frameshift due to insertion around 550778. Nonsense mutation around 550817, 551504.", "psc": { "uniref90_id": "UniRef90_UPI002FCE1E4D", "product": "MATE family efflux transporter", "uniref50_id": "UniRef50_UPI002FCE1E4D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FCE1E4D", "UniRef:UniRef50_UPI002FCE1E4D" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FCE1E4D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FCE1E4D" ], "product": "MATE family efflux transporter" } }, "id": "JPCNHPFPBM_999", "locus": "GCA000292505_00999" }, { "type": "cds", "sequence": "contig_1", "start": 551502, "stop": 551930, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAACAGTAAAAGGGATCGTTTTGAAAAACAACTCCTAATTAAAAATGTTTTTGAATCAAAACAGCTTTTTCTAACTATTCTTAGATTTACTGTTCCAACTTTTTTCTTTGCACTTTTTAGTGCTGCTTATATATTTATAGATCAAATCATGGTGATTAAGTTTGTTCCTAGAAGTGAACTTAATCCTGATTCTATTTTTACTGATCAGAGTTTAATTGATGAATTTAAAAATTCTGCTTTTTATAAAAGTGATAGTTTTTTAAGCTCTGGACTTAATATTAAACAATTTATTAAAACTGTTTTAAATATTAGTCAACCTTTGATTGTTTTGTTAAACGCCATTAATATTTTTATTCCTCTAGGAACTGGAGTTATTTTTTCTAAAGCAATTGGTAGAAATGATCAAAATAAAATTCAGGAAGCTTGA", "aa": "MNSKRDRFEKQLLIKNVFESKQLFLTILRFTVPTFFFALFSAAYIFIDQIMVIKFVPRSELNPDSIFTDQSLIDEFKNSAFYKSDSFLSSGLNIKQFIKTVLNISQPLIVLLNAINIFIPLGTGVIFSKAIGRNDQNKIQEA", "aa_hexdigest": "a76f31537c9fecd0e1b1aa5e553fe29c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16294.84609999998, "isoelectric_point": 9.474934577941895 }, "id": "JPCNHPFPBM_1000", "locus": "GCA000292505_01000" }, { "type": "cds", "sequence": "contig_1", "start": 551931, "stop": 552116, "strand": "-", "frame": 2, "gene": null, "product": "peptide-methionine (R)-S-oxide reductase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAU5ZP34" ], "nt": "ATGATAAGAACTGAAGTTCGTGCTAAAAACAGTGATAGCCACTTGGGACATGTTTTTAATGATGGACCGAGTGAATTAGGTGGATTGCGCTATTGTATTAATTCTGCAGCTTTAAAATTTATTCCTTTTGAAGAGATGGAAAAATTAGGATACAAGGATTACATCCATCTTTTTAAAAATAAATAA", "aa": "MIRTEVRAKNSDSHLGHVFNDGPSELGGLRYCINSAALKFIPFEEMEKLGYKDYIHLFKNK", "aa_hexdigest": "cb3735859ffda3bb46315f0f2a15d0ca", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AAU5ZP34", "query_cov": 0.9508196721311475, "subject_cov": 0.8656716417910447, "identity": 0.638, "score": 86.3, "evalue": 4.27e-21, "valid": false, "product": "peptide-methionine (R)-S-oxide reductase", "uniref50_id": "UniRef50_A0AAU5ZP34" }, "pscc": { "uniref50_id": "UniRef50_A0AAU5ZP34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAU5ZP34" ], "product": "peptide-methionine (R)-S-oxide reductase" }, "genes": [], "id": "JPCNHPFPBM_1001", "locus": "GCA000292505_01001" }, { "type": "cds", "sequence": "contig_1", "start": 552180, "stop": 552383, "strand": "-", "frame": 2, "gene": "msrB", "product": "Peptide methionine sulfoxide reductase MsrB", "db_xrefs": [ "COG:COG0229", "COG:O", "EC:1.8.4.12", "GO:0006979", "GO:0030091", "GO:0033743", "SO:0001217", "UniRef:UniRef50_Q8E026", "UniRef:UniRef90_P47686" ], "nt": "ATGAGTAAATACCAAAAAAAGAGTGAAGCTGAGCTAAAACGTACTTTAACCAAACTCCAGTTTGATGTAACGCAAAATGCTCACACTGAGCCACCTTACATTAATGAATATAACCGCAATTTTGAAAAGGGAATTTATGTTGATATAACATCTGGTGAACCACTTTTTATTTCAACTGATAAATTTAAATCTGGTTGTGGTTGA", "aa": "MSKYQKKSEAELKRTLTKLQFDVTQNAHTEPPYINEYNRNFEKGIYVDITSGEPLFISTDKFKSGCG", "aa_hexdigest": "0f07e3d21a7b38b6e7204dc83e7933ea", "start_type": "ATG", "rbs_motif": null, "genes": [ "msrB" ], "truncated": "5-prime", "pseudogene": { "start": 551931, "stop": 552383, "observations": { "mutation": [ 552182 ], "stop": [ 552182 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47686", "query_cov": 1.0, "subject_cov": 0.44666666666666666, "identity": 1.0, "score": 139.0, "evalue": 4.02e-41, "gene_start": 1, "gene_end": 67, "reference_start": 1, "reference_end": 67, "reference_sequence": "MSKYQKKSEAELKRTLTKLQFDVTQNAHTEPPYINEYNRNFEKGIYVDITSGEPLFISTDKFKSGCGWPAFSKPIDKNLIANYRDESHGMIRTEVRAKNSDSHLGHVFNDGPSELGGLRYCINSAALKFIPFEEMEKLGYKDYIHLFKNK" }, "paralog": false, "identity": 0.9933333333333333, "score": 308.0, "evalue": 5.5e-110, "description": "Internal stop codon at 552182. Nonsense mutation around 552182.", "psc": { "uniref90_id": "UniRef90_P47686", "gene": "msrB", "product": "Peptide methionine sulfoxide reductase MsrB", "ec_ids": [ "1.8.4.12" ], "uniref50_id": "UniRef50_Q8E026", "cog_id": "COG0229", "cog_category": "O", "go_ids": [ "GO:0033743", "GO:0030091", "GO:0006979" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47686", "EC:1.8.4.12", "UniRef:UniRef50_Q8E026", "COG:COG0229", "COG:O", "GO:0033743", "GO:0030091", "GO:0006979" ] }, "pscc": { "uniref50_id": "UniRef50_Q8E026", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8E026" ], "product": "Peptide methionine sulfoxide reductase MsrB" } }, "id": "JPCNHPFPBM_1002", "locus": "GCA000292505_01002" }, { "type": "cds", "sequence": "contig_1", "start": 552380, "stop": 552781, "strand": "-", "frame": 3, "gene": "ytpR", "product": "YtpR family tRNA-binding protein", "db_xrefs": [ "GO:0000049", "SO:0001217", "UniRef:UniRef50_P47687", "UniRef:UniRef90_P47687" ], "nt": "ATGAAAAGAATAAGTGAGCTTTTAGGTTATGATCTTATTTCATTAGCAAATAAAGTTCCTTTTGTTGTTTGTGAAGTTGTTTCAGTTATTCCTATTGCAAACACCCATCTAAAAAGGTGTAAAGTTAACACTGGTTTGACAAAAAGCTTGGATATTGTTTGTGGAGCTAATAATGTTAGAGTTGGTATGAAAACAGTTTTAGTACAAGTAGGTGGAGTGTTACCTAGCGGTACAGTTATCAAAAAAACTAAAATAGCTGGTTTTGATTCAGTTGGCATGTTATGTTCTGAAAAAGAACTTAATTTAAAACAGAAAAATGAGGGTATAATTGAGATTAATCCTAAGATCAAAGTAGGAAAACCATTTATTGATGTATATTTAAATAAACAACATTCCAAATGA", "aa": "MKRISELLGYDLISLANKVPFVVCEVVSVIPIANTHLKRCKVNTGLTKSLDIVCGANNVRVGMKTVLVQVGGVLPSGTVIKKTKIAGFDSVGMLCSEKELNLKQKNEGIIEINPKIKVGKPFIDVYLNKQHSK", "aa_hexdigest": "d4902a162519a9d81a112745d4fbe60f", "start_type": "ATG", "rbs_motif": null, "genes": [ "ytpR" ], "truncated": "both", "pseudogene": { "start": 552347, "stop": 553033, "observations": { "mutation": [ 552382, 552910 ], "stop": [ 552382, 552910 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47687", "query_cov": 1.0, "subject_cov": 0.5833333333333334, "identity": 1.0, "score": 258.0, "evalue": 1.44e-85, "gene_start": 1, "gene_end": 133, "reference_start": 85, "reference_end": 217, "reference_sequence": "MFDISKDFVSIFYRKETLKNCMFGIIGSRSETTLRQQNDKDWSFFVNDANRITGFNFFNIKKSFKKFFLSHRFNEGLNYPSLKIMKRISELLGYDLISLANKVPFVVCEVVSVIPIANTHLKRCKVNTGLTKSLDIVCGANNVRVGMKTVLVQVGGVLPSGTVIKKTKIAGFDSVGMLCSEKELNLKQKNEGIIEINPKIKVGKPFIDVYLNKQHSKWVNTKKRVKLS" }, "paralog": false, "identity": 0.9912280701754386, "score": 446.0, "evalue": 2.41e-162, "description": "Internal stop codon at 552382, 552910. Nonsense mutation around 552382, 552910.", "psc": { "uniref90_id": "UniRef90_P47687", "gene": "ytpR", "product": "YtpR family tRNA-binding protein", "uniref50_id": "UniRef50_P47687", "go_ids": [ "GO:0000049" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47687", "UniRef:UniRef50_P47687", "GO:0000049" ] }, "pscc": { "uniref50_id": "UniRef50_P47687", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47687" ], "product": "Uncharacterized protein MG449" } }, "id": "JPCNHPFPBM_1003", "locus": "GCA000292505_01003" }, { "type": "cds", "sequence": "contig_1", "start": 552908, "stop": 553033, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGATATATCTAAAGATTTTGTTTCTATCTTTTATCGTAAAGAAACATTAAAAAACTGTATGTTTGGCATCATAGGAAGTAGAAGTGAAACAACTTTACGCCAACAAAATGACAAAGACTGA", "aa": "MFDISKDFVSIFYRKETLKNCMFGIIGSRSETTLRQQNDKD", "aa_hexdigest": "0beeec6f9cc5fde636670048409328d5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4863.506200000002, "isoelectric_point": 7.900616645812989 }, "id": "JPCNHPFPBM_1004", "locus": "GCA000292505_01004" }, { "type": "cds", "sequence": "contig_1", "start": 553043, "stop": 553312, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAGTTTTTTAACAAAGCACAATCTGCTAGCAGTGCTCATAAGTTAGCAGTTCAATTTTTAGAAAAAAAGCTATTAAAAAAAGAAGTTAATTTCAGAAGAGCGGCTTTGTTGACAACAATGTTTGAAACTGATAAAAATCAACTCATAAAGCAAGAATTTGTTACGCTTCTCAACAATGCAGTTGATGTAAGTGAGCACTTGCTTTCACCTGTAATTTGTACACACACAGGTATCAATAGTTATGCCTTTTTAATACAAACAGAATAA", "aa": "MQFFNKAQSASSAHKLAVQFLEKKLLKKEVNFRRAALLTTMFETDKNQLIKQEFVTLLNNAVDVSEHLLSPVICTHTGINSYAFLIQTE", "aa_hexdigest": "30e4fe475650da5e6f639ddfeeb58777", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10142.645699999992, "isoelectric_point": 8.853910636901855 }, "id": "JPCNHPFPBM_1005", "locus": "GCA000292505_01005" }, { "type": "cds", "sequence": "contig_1", "start": 553607, "stop": 553915, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCGGATCGCTTTTTTAGTTGATTCTGTCAGTAATCTAAAGGAAGATAAAAATAGTCATCTCTATGTTTTATCGCTTTATATTATTGAAACAATTGCTGAACACCAAGAAACATTCAAATCAGGTTTTAATATTGATCTTAAAACCTTAACTGATAAGATGATTAACGCCCCTAAAGGTGTTAAGTTTTCAACATCGCAAACTTCTGAAGAAGAGGTTCGTGACAAAGTTAAGAGCATAATTAATGATTATGATCTTATCATAGGAATCCCTATTGATAAAGAGATCTCAACCTCCTATTTAAATTGA", "aa": "MRIAFLVDSVSNLKEDKNSHLYVLSLYIIETIAEHQETFKSGFNIDLKTLTDKMINAPKGVKFSTSQTSEEEVRDKVKSIINDYDLIIGIPIDKEISTSYLN", "aa_hexdigest": "8abaa85f83670ec0e02791a4b8d68771", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11613.10229999999, "isoelectric_point": 5.078984642028807 }, "id": "JPCNHPFPBM_1006", "locus": "GCA000292505_01006" }, { "type": "cds", "sequence": "contig_1", "start": 553918, "stop": 555102, "strand": "-", "frame": 1, "gene": "tuf", "product": "elongation factor Tu", "db_xrefs": [ "COG:COG0050", "COG:J", "GO:0001968", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006414", "GO:0009897", "GO:0016020", "RefSeq:WP_009885583.1", "SO:0001217", "UniParc:UPI0000129DB5", "UniRef:UniRef100_P13927", "UniRef:UniRef50_P23568", "UniRef:UniRef90_P23568" ], "nt": "ATGGCAAGAGAGAAATTTGACCGTTCCAAACCACATGTCAATGTTGGTACCATTGGTCACATTGACCATGGTAAAACCACTTTAACAGCTGCTATCTGTACAGTTTTAGCAAAGGAAGGAAAATCAGCTGCAACGCGTTATGATGAAATTGATAAAGCCCCTGAAGAAAAAGCAAGGGGAATTACAATTAACTCTGCACACGTAGAATATTCTTCTGACAAACGTCACTATGCCCATGTTGACTGTCCTGGACATGCTGACTACATTAAAAACATGATCACAGGTGCTGCACAAATGGATGGAGCTATTCTAGTTGTTTCAGCAACTGATAGTGTGATGCCCCAAACCCGCGAGCACATCTTACTTGCCCGCCAAGTAGGGGTTCCTAAAATGGTAGTTTTTCTAAACAAGTGTGATATTGCTAGTGATGAAGAGGTACAAGAACTTGTTGCTGAAGAAGTACGTGATCTGTTAACTTCCTATGGTTTTGATGGTAAGAACACTCCTATTATTTATGGCTCAGCTTTAAAAGCATTGGAAGGTGATCCAAAGTGGGAGGCTAAGATCCATGATTTGATTAAAGCAGTTGATGAATGGATTCCAACTCCTACACGTGAAGTAGATAAACCTTTCTTATTAGCAATTGAAGATACGATGACCATTACTGGTAGAGGTACAGTTGTTACAGGAAGAGTTGAAAGAGGTGAACTCAAAGTAGGTCAAGAAGTTGAAATTGTTGGTTTAAAACCAATTAGAAAAGCAGTTGTTACTGGAATTGAAATGTTCAAAAAGGAACTTGATTCAGCAATGGCTGGTGACAATGCTGGGGTATTATTACGTGGTGTTGAACGTAAAGAAGTTGAAAGAGGTCAAGTTTTAGCAAAACCAGGCTCTATTAAACCGCACAAGAAATTTAAAGCTGAGATCTATGCTTTAAAGAAAGAAGAAGGTGGCAGACACACTGGTTTTTTAAACGGTTACCGTCCTCAATTCTATTTCCGTACCACTGATGTAACTGGTTCTATTGCTTTAGCTGAAAATACTGAAATGGTTCTACCTGGTGATAATGCTTCTATTACTGTTGAGTTAATTGCTCCTATTGCTTGTGAAAAAGGTAGTAAGTTCTCAATTCGTGAAGGTGGCAGAACTGTAGGGGCAGGCACTGTAACAGAAGTTCTAGAATAA", "aa": "MAREKFDRSKPHVNVGTIGHIDHGKTTLTAAICTVLAKEGKSAATRYDEIDKAPEEKARGITINSAHVEYSSDKRHYAHVDCPGHADYIKNMITGAAQMDGAILVVSATDSVMPQTREHILLARQVGVPKMVVFLNKCDIASDEEVQELVAEEVRDLLTSYGFDGKNTPIIYGSALKALEGDPKWEAKIHDLIKAVDEWIPTPTREVDKPFLLAIEDTMTITGRGTVVTGRVERGELKVGQEVEIVGLKPIRKAVVTGIEMFKKELDSAMAGDNAGVLLRGVERKEVERGQVLAKPGSIKPHKKFKAEIYALKKEEGGRHTGFLNGYRPQFYFRTTDVTGSIALAENTEMVLPGDNASITVELIAPIACEKGSKFSIREGGRTVGAGTVTEVLE", "aa_hexdigest": "61ef27a09c235dba715d0dea52d18464", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000129DB5", "ncbi_nrp_id": "WP_009885583.1", "uniref100_id": "UniRef100_P13927", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000129DB5", "RefSeq:WP_009885583.1", "UniRef:UniRef100_P13927" ] }, "ips": { "uniref100_id": "UniRef100_P13927", "uniref90_id": "UniRef90_P23568" }, "psc": { "uniref90_id": "UniRef90_P23568", "gene": "tuf", "product": "elongation factor Tu", "uniref50_id": "UniRef50_P23568", "cog_id": "COG0050", "cog_category": "J", "go_ids": [ "GO:0001968", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006414", "GO:0009897", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_P23568", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P23568" ], "product": "Elongation factor Tu" }, "genes": [ "tuf" ], "id": "JPCNHPFPBM_1007", "locus": "GCA000292505_01007" }, { "type": "cds", "sequence": "contig_1", "start": 555272, "stop": 555421, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTCACTAGTGCTTTTGTTTTAACTGGAATTGTTGAAAGCCTTTTAACATTTGGAACTATTATTGAACAAATTGATAAATTCACTGATCAGACTAATGTGATGTTATTAATTTATGCAGTTATCTACACTTTTAATCCAAAAAGTTGA", "aa": "MVTSAFVLTGIVESLLTFGTIIEQIDKFTDQTNVMLLIYAVIYTFNPKS", "aa_hexdigest": "78fe6aba90e9a10c824b534ff9edf733", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5487.3813, "isoelectric_point": 4.316091728210449 }, "id": "JPCNHPFPBM_1008", "locus": "GCA000292505_01008" }, { "type": "cds", "sequence": "contig_1", "start": 555422, "stop": 555799, "strand": "+", "frame": 2, "gene": null, "product": "putative protein MG452", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P75125", "UniRef:UniRef90_P47690" ], "nt": "TTGTTAAAAAACCAACAATTCTTTTTAAGTGCATTAGCTTATATATTATTTACTTTTATTGGCTATAACCTAATTTTGTCAATAGCTGGTATAGCTTATAAATCAACAAATCCATATAAGTTAACAAGTAGTATTTTTCTCCATGTAATTGCACCAATAGCATTCTTCATAGCAAGTTTTATCAAAATAAAACATGAGAAAGATGTCAATATTAACATGTTCTTTAAAAGCCTATTATTATTCATGATCTATCCTTTAATATATGGGCTTTATTTAGTAACTATTCCATATGTAAGGCATTATCTTTTTAATGGTAGGCCATCTACTTATACAATTTATGGCAGCATTACAAATACTAAAGATAATCCTTTTGCTTGA", "aa": "MLKNQQFFLSALAYILFTFIGYNLILSIAGIAYKSTNPYKLTSSIFLHVIAPIAFFIASFIKIKHEKDVNINMFFKSLLLFMIYPLIYGLYLVTIPYVRHYLFNGRPSTYTIYGSITNTKDNPFA", "aa_hexdigest": "ae7f3cd4bacbeb2d438900d641a82cd6", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 555155, "stop": 555940, "observations": { "mutation": [ 555215, 555218, 555419, 555797, 555926 ], "stop": [ 555215, 555218, 555419, 555797, 555926 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47690", "query_cov": 1.0, "subject_cov": 0.4789272030651341, "identity": 0.9840000000000001, "score": 238.0, "evalue": 1.74e-77, "gene_start": 1, "gene_end": 125, "reference_start": 90, "reference_end": 214, "reference_sequence": "MLIIFKDLNLVKTNNCQIIGWWKQLSLPQKTFLSFIPLFLVTSAFVLTGIVESLLTFGTIIEQIDKFTDQTNVMLLIYAVIYTFNPKSWLLKNQQFFLSALAYILFTFIGYNLILSIAGIAYKSTNPYKLTSSIFLHVIAPIAFFIASFIKIKHEKDVNINMFFKSLLLFMIYPLIYGLYLVTIPYVRHYLFNGRPSTYTIYGSITNTKNNPFAWLVVFAVLFIYFPLSYLAIYLLQLKLIKKAIQPQFNLPFTLNKWKQK" }, "paralog": false, "identity": 0.9731800766283525, "score": 490.0, "evalue": 1.8e-179, "description": "Internal stop codon at 555215, 555218, 555419, 555797, 555926. Nonsense mutation around 555215, 555218, 555419, 555797, 555926.", "psc": { "uniref90_id": "UniRef90_P47690", "product": "Uncharacterized protein MG452", "uniref50_id": "UniRef50_P75125", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47690", "UniRef:UniRef50_P75125", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P75125", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75125" ], "product": "Uncharacterized protein MG452 homolog" } }, "id": "JPCNHPFPBM_1009", "locus": "GCA000292505_01009" }, { "type": "cds", "sequence": "contig_1", "start": 555925, "stop": 556803, "strand": "+", "frame": 1, "gene": "galU", "product": "UTP--glucose-1-phosphate uridylyltransferase GalU", "db_xrefs": [ "COG:COG1210", "COG:M", "EC:2.7.7.9", "GO:0003983", "GO:0006011", "GO:0009058", "RefSeq:WP_009885581.1", "SO:0001217", "UniParc:UPI0000053FBB", "UniRef:UniRef100_P47691", "UniRef:UniRef50_P47691", "UniRef:UniRef90_P47691" ], "nt": "ATGAAAACAAAAATAAGAAAAGCAGTTATTCCTGCTGCTGGGTTGGGTGTTAGGTTACTACCAGCAACAAAAGCAATTCCCAAAGAGATGTTACCATTGGTAAATAAACCTACTATCCAATACATAGTAGAGGAAGCAGTTAAAAGTGGCATTGAACAGATTCTTGTCATTGTTTCATCCAAAAAAACAGCTATATTAGATCATTTTGATTATGATCTGATCTTAGAAAATGCCTTAATTCAAAAAAATAAATTGCAGGAGCATAAAGAGATTGAAGATATTGCTAATTTAGCACATATCTTTTTTGTTAGACAAAAAAATCAAGATGGTTTGGGAGATGCAATCTTGTTTGCTGAATCTTTTGTTGGTAATGAAGACTTTGCAGTATTGTTAGGTGATGATGTTGTTTTTAGTAAAGAACCTGCTTTAAAACAATGCTTGGAAGCTTATTATGAAACTAATTGTCAAACAATCGGTGTACAAGAAGTAGATCCTTGTCATGTTGATAAGTATGGAATTATCACCCCTGAAGGTGATTACAAAAATAAAGATCTTATTAAGGTTTTAGCAATGACTGAAAAACCTAAACCAAAAGATGCTAAAAGTAATTTAGCAATCTTAGGGCGATATGTACTCAAACCATCTATTTTCAAAGCACTTAGAAGTGTACCTTATGGAGTTGGTGGTGAGTTGCAGCTAACTGATGGTTTAAATTTTTGTTTGAAAAATGAAAACTTTTATGCAAGAAAGTTTACTGGTACTAGGTTTGATGTTGGCACAAAGAGTGGTTTTATTAAAGCAAATTTATTTACTGCTTTAAACAATAAAGATATTAGTAAAAAAGAAGTTTTAGAACTTTTAAATTTAGTTAAAGCTTAA", "aa": "MKTKIRKAVIPAAGLGVRLLPATKAIPKEMLPLVNKPTIQYIVEEAVKSGIEQILVIVSSKKTAILDHFDYDLILENALIQKNKLQEHKEIEDIANLAHIFFVRQKNQDGLGDAILFAESFVGNEDFAVLLGDDVVFSKEPALKQCLEAYYETNCQTIGVQEVDPCHVDKYGIITPEGDYKNKDLIKVLAMTEKPKPKDAKSNLAILGRYVLKPSIFKALRSVPYGVGGELQLTDGLNFCLKNENFYARKFTGTRFDVGTKSGFIKANLFTALNNKDISKKEVLELLNLVKA", "aa_hexdigest": "1107b311c79eb86ee11611e83b4f1807", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000053FBB", "ncbi_nrp_id": "WP_009885581.1", "uniref100_id": "UniRef100_P47691", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000053FBB", "RefSeq:WP_009885581.1", "UniRef:UniRef100_P47691" ] }, "ips": { "uniref100_id": "UniRef100_P47691", "uniref90_id": "UniRef90_P47691" }, "psc": { "uniref90_id": "UniRef90_P47691", "gene": "galU", "product": "UTP--glucose-1-phosphate uridylyltransferase GalU", "ec_ids": [ "2.7.7.9" ], "uniref50_id": "UniRef50_P47691", "cog_id": "COG1210", "cog_category": "M", "go_ids": [ "GO:0003983", "GO:0006011", "GO:0009058" ] }, "pscc": { "uniref50_id": "UniRef50_P47691", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47691" ], "product": "UTP--glucose-1-phosphate uridylyltransferase" }, "genes": [ "galU" ], "id": "JPCNHPFPBM_1010", "locus": "GCA000292505_01010" }, { "type": "cds", "sequence": "contig_1", "start": 556793, "stop": 557215, "strand": "-", "frame": 3, "gene": "osmC", "product": "Organic hydroperoxide resistance protein-like", "db_xrefs": [ "COG:COG1764", "COG:V", "EC:1.11.1.28", "GO:0006979", "KEGG:K04063", "RefSeq:WP_014894703.1", "SO:0001217", "UniParc:UPI00027B4091", "UniRef:UniRef100_UPI00027B4091", "UniRef:UniRef50_P47692", "UniRef:UniRef90_P47692" ], "nt": "ATGGCATTGATTTATAAAACTGTTGCACAAACTGAAACTGGGAGAGAAGGTAGTGTTAAAACTTTAGATGGTTTTCAAACAAAACTTAGTTTTCCCAAACCTGATTTATCAGTTCAAACAGAAAATAATCCTGAGCAGTTATTTGCATCTGCTTATGCAAGTTGTTTTTCTCAAGCAGTAATTGTTGTTATGCAACAACATCAATTTAGTTTTTCAAAAAAACCAGTTGTAAGTGTTAAAGTAGAACTCCATCAAGAAAATGGACTATTTCACCTTAAAGCTGGTGTTGAATTAACTACTAATAGTAATGACCAAGAAGTTGGTAAAAAACTAATCAAAAAAGCCCATGAAATGTGTCCTTTTAGTCGATTGATTAGAAGTGAAAACTTCCTTGGTTTAACTTTAAATGGTATTAAGCTTTAA", "aa": "MALIYKTVAQTETGREGSVKTLDGFQTKLSFPKPDLSVQTENNPEQLFASAYASCFSQAVIVVMQQHQFSFSKKPVVSVKVELHQENGLFHLKAGVELTTNSNDQEVGKKLIKKAHEMCPFSRLIRSENFLGLTLNGIKL", "aa_hexdigest": "7a084df08efd3d42d8893563bcd87426", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B4091", "ncbi_nrp_id": "WP_014894703.1", "uniref100_id": "UniRef100_UPI00027B4091", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B4091", "RefSeq:WP_014894703.1", "UniRef:UniRef100_UPI00027B4091" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B4091", "uniref90_id": "UniRef90_P47692" }, "psc": { "uniref90_id": "UniRef90_P47692", "gene": "OsmC", "product": "Organic hydroperoxide resistance protein-like", "ec_ids": [ "1.11.1.28" ], "uniref50_id": "UniRef50_P47692", "cog_id": "COG1764", "cog_category": "V", "kegg_orthology_id": "K04063", "go_ids": [ "GO:0006979" ] }, "pscc": { "uniref50_id": "UniRef50_P47692", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47692" ], "product": "Organic hydroperoxide resistance protein-like" }, "genes": [ "osmC" ], "id": "JPCNHPFPBM_1011", "locus": "GCA000292505_01011" }, { "type": "cds", "sequence": "contig_1", "start": 557248, "stop": 557613, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAATAACATATTGCAATTTCTCAAAGAAAGAGAACTTTATTCACAAGCTAATTTTGAAACAGAACTAGATAACCATTTAAAAGAGAAAAAAATTAACTTTTATGTTGGTTTTGATCCAACTGCTAATTCTTTACATATTGGCAATTATGTTTTAATTCACATTGCAAAATTATTAAAAGACATGGGGCATACTCCGCACATAGTTCTGGGGAGTGCAACTGCTTTAATTGGTGATCCTACTGGCAGAATTGAATTAAGGAAAATTTTAGAAGAAAAAGAAATTGTAAAAAACACCAAAACAATTAAACAACAAATCAAACAGTTTTTAGGTGATGTAATTATTCATGAAAACAAAGTTTGA", "aa": "MLNNILQFLKERELYSQANFETELDNHLKEKKINFYVGFDPTANSLHIGNYVLIHIAKLLKDMGHTPHIVLGSATALIGDPTGRIELRKILEEKEIVKNTKTIKQQIKQFLGDVIIHENKV", "aa_hexdigest": "7506d3fd3d80ad3a4ca0b1711229815c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13888.984899999983, "isoelectric_point": 7.988938331604004 }, "id": "JPCNHPFPBM_1012", "locus": "GCA000292505_01012" }, { "type": "cds", "sequence": "contig_1", "start": 557728, "stop": 557952, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATTAAACTATATGATCTTACAAGCATATGACTTTTATTATCTACATAAAAACCATAATGTCACTTTACAAATAGGTGGAAGTGATCAGTGGGCTAATATTTTGGCTGGTGCTAACTTAATTAAAAGAAAAAATAATGCTAATGTTTTTGGATTAACTGCTAATTTATTAGTTAAAGCTAACGGAGAAAAAATGGGTAAAACTAGTAGCGGAGCATTATGA", "aa": "MELNYMILQAYDFYYLHKNHNVTLQIGGSDQWANILAGANLIKRKNNANVFGLTANLLVKANGEKMGKTSSGAL", "aa_hexdigest": "ce7de688b2ad878ca4e0a0ef06979c51", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8172.2934, "isoelectric_point": 9.398539543151855 }, "id": "JPCNHPFPBM_1013", "locus": "GCA000292505_01013" }, { "type": "cds", "sequence": "contig_1", "start": 558037, "stop": 558369, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01479.30", "PFAM:PF22421.1" ], "nt": "ATGCTAACAATGCTTGATAAAAAAGTAATAGATGAATTGTGTAATTTAAAAGGCCCAAAAATTAAACAAACCAAGCAAATGCTAGCCTTTTTAATTACTGAATTAGTCCATGGCACTAAAAAAGCAAAAGAAGCACAACAACGATCTGAACTAATATTTAGTAATCAACCAGATCTTGATATTAAGTTAGTAAAAACAAGCACTAATCTGATTGATTATTTAGTTGAAACTAAATTTATTAAAAGTAAATCAGAAGCAAGAAGATTAATTAGTCAAAAAGGTTTGACAATTAACAATAAACACGTTTTGGACTTAAACCAAATAATTGAATGA", "aa": "MLTMLDKKVIDELCNLKGPKIKQTKQMLAFLITELVHGTKKAKEAQQRSELIFSNQPDLDIKLVKTSTNLIDYLVETKFIKSKSEARRLISQKGLTINNKHVLDLNQIIE", "aa_hexdigest": "92a5cebc32e184035a6875b868cb11e9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01479.30", "name": "S4", "length": 39, "aa_cov": 0.35454545454545455, "hmm_cov": 1.0, "evalue": 1.347491975961269e-09, "score": 32.761024475097656, "start": 71, "stop": 109 }, { "id": "PF22421.1", "name": "SYY_C-terminal", "length": 57, "aa_cov": 0.5181818181818182, "hmm_cov": 0.9649122807017544, "evalue": 6.19235781241365e-11, "score": 37.22304153442383, "start": 53, "stop": 109 } ], "seq_stats": { "molecular_weight": 12620.776799999983, "isoelectric_point": 9.519611167907716 }, "id": "JPCNHPFPBM_1014", "locus": "GCA000292505_01014" }, { "type": "cds", "sequence": "contig_1", "start": 558427, "stop": 559143, "strand": "-", "frame": 1, "gene": null, "product": "putative protein MG456", "db_xrefs": [ "GO:0005886", "SO:0001217", "UniRef:UniRef50_P47694", "UniRef:UniRef90_P47694" ], "nt": "ATGGGTAATTTCAAGGCAATAGAATCATTTGAATTTTTTGAACAAGGTCCTATAAGCAGCGATATATTACCAAGTTTTTATCCAACTGCTCTTTACAATTTTGAACCACTTCCAAGACAATTTAAAGTTACTTATAAAGATGGTAGTGAATTTGTTTTTGGTCATATTTATGCAATTAAAAGAAGCAGTAATAAAACAGAAAAAGTAGCTTGCTTGATTGCTATTACACCAGCAATTAATAGTGAAAATCATTTCTTTTTAACTGATGCTAACTATTCTTTAAATAAGAATGTGGCTCAGTTTGAAGCACTTACTGAAAACAAAAAGCAAGAAAACATCTCACTTTTTGTTGAAAAAGATTCAAATTTTTCTTTTCAGAATTTAGATACAGAGGTATTAAATAAAGTGCTTTTTAATCCTTTGAATGTATATGCCGAATTTAATGTCTATAATGACACAGTTCATACTTACTTGTTTATGAGTGTGCCTACAACCTTTATGGATACTAAACTAAAAGTGAATGAAGCATTTGATGATCTAGTTACAAACATTAAACTCCAAGCAAGCTATGATTTCAAAACATTAAATTCAATGCAGAAAGTTGTTGATTTACTTCATAACAAATTAATCAAAAAACCAGAAAGTAAAAGCTCTAGTCAAAAAAGTGTTGAAACTGAAATAGAAAAAGAAGTTAAAGATAAATTAGCTAAGAATTAA", "aa": "MGNFKAIESFEFFEQGPISSDILPSFYPTALYNFEPLPRQFKVTYKDGSEFVFGHIYAIKRSSNKTEKVACLIAITPAINSENHFFLTDANYSLNKNVAQFEALTENKKQENISLFVEKDSNFSFQNLDTEVLNKVLFNPLNVYAEFNVYNDTVHTYLFMSVPTTFMDTKLKVNEAFDDLVTNIKLQASYDFKTLNSMQKVVDLLHNKLIKKPESKSSSQKSVETEIEKEVKDKLAKN", "aa_hexdigest": "39bf944ed9f9c3a84d3fb61fee5b0bc8", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 558427, "stop": 559431, "observations": { "mutation": [ 559155 ], "stop": [ 559155 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47694", "query_cov": 1.0, "subject_cov": 0.7125748502994012, "identity": 0.996, "score": 458.0, "evalue": 2.79e-161, "gene_start": 1, "gene_end": 238, "reference_start": 97, "reference_end": 334, "reference_sequence": "MSDLTKFNKFFLTPNKLNAFLRVIGLCGLFSVIAISFGIYSYTRNEIPNIASLFLIVLGSVVLFLAFVIHFAALFKRNKLLKKVNKENRTNLWQKEMGNFKAIESFEFFEQGPISSDILPSFYPTALYNFEPLPRQFKVTYKDGSEFVFGHIYAIKRSSNKTEKVACLIAITPAINSENHFFLTDANYSLNKNVAQFEALTENKKQENISLFVEKDSNFSFQNLDTEVLNKVLFNPLNVYAKFNVYNDTVHTYLFMSVPTTFMDTKLKVNEAFDDLVTNIKLQASYDFKTLNSMQKVVDLLHNKLIKKPESKSSSQKSVETEIEKEVKDKLAKN" }, "paralog": false, "identity": 0.9940119760479041, "score": 652.0, "evalue": 1.67e-240, "description": "Internal stop codon at 559155. Nonsense mutation around 559155.", "psc": { "uniref90_id": "UniRef90_P47694", "product": "Uncharacterized protein MG456", "uniref50_id": "UniRef50_P47694", "go_ids": [ "GO:0005886" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47694", "UniRef:UniRef50_P47694", "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P47694", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47694" ], "product": "Uncharacterized protein MG456" } }, "id": "JPCNHPFPBM_1015", "locus": "GCA000292505_01015" }, { "type": "cds", "sequence": "contig_1", "start": 559153, "stop": 559431, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGATCTAACTAAATTTAACAAGTTCTTTTTGACACCAAATAAACTCAATGCTTTTCTAAGAGTAATTGGACTTTGTGGTCTTTTTAGTGTTATTGCTATCTCATTTGGTATTTATAGTTATACACGTAATGAAATACCAAATATAGCATCACTTTTTTTAATTGTTTTAGGTAGTGTTGTTTTGTTTTTAGCATTTGTTATCCATTTTGCTGCTCTTTTTAAAAGAAACAAGCTATTAAAAAAAGTTAACAAAGAAAACAGAACTAATTTATGA", "aa": "MSDLTKFNKFFLTPNKLNAFLRVIGLCGLFSVIAISFGIYSYTRNEIPNIASLFLIVLGSVVLFLAFVIHFAALFKRNKLLKKVNKENRTNL", "aa_hexdigest": "85b08d3165963612172bb14e8b2f55bd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10457.435899999984, "isoelectric_point": 10.358344841003415 }, "id": "JPCNHPFPBM_1016", "locus": "GCA000292505_01016" }, { "type": "cds", "sequence": "contig_1", "start": 559779, "stop": 560000, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAACAAACAGTTAGTGTTGTTGATTTTATTGATAACTTCTTGGTTAACTTACTATCATTTAAGCAAGAATTAAATGCAATAGAAGTTGGTTATGGTAATGAAGCTTGGAATTGTTTTAAAGTACTAAATGATCAAAGTAAAACAATTACTGATTTTTTTACAGTAATTAAAAATAATCCCCAATTGTTTGACAGTGAATATCACTACATTTTGATTTGA", "aa": "MKQTVSVVDFIDNFLVNLLSFKQELNAIEVGYGNEAWNCFKVLNDQSKTITDFFTVIKNNPQLFDSEYHYILI", "aa_hexdigest": "d49fdaf039ab9cdc6a2134aa7bc293af", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8524.595899999998, "isoelectric_point": 4.5550428390502935 }, "id": "JPCNHPFPBM_1017", "locus": "GCA000292505_01017" }, { "type": "cds", "sequence": "contig_1", "start": 559984, "stop": 561411, "strand": "-", "frame": 1, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V6LLW0" ], "nt": "GTGAAATTTACCAATATTGCTGGACTTCAAGAGGAAAAGCATGAGTTGCTTGAGATAGTTGATTATTTAAAAAATCCATTGAAATATGCCCAGATGGGAGCAAGATCCCCACGTGGGGTAATTTTATATGGTCCACCTGGGACAGGTAAAACATTATTAGCTAAAGCAGTAGCTGGTGAAGCTGGTGTTCCTTTCTTTCAATCAACGGGTTCTGGATTTGAAGATATGCTTGTTGGTGTTGGTGCTAAACGGGTTAGAGATCTTTTCAATAAAGCTAAAAAGGCTGCTCCTTGTATTATTTTTATTGATGAAATTGATTCAGTTGGTTCTAAACGGGGTAGAGTTGAACTCTCTTCTTATTCTGTTGTTGAGCAAACCTTAAACCAATTGTTAGCTGAAATGGATGGATTTACAAGCAGAACAGGTGTTGTTGTAATGGCAGCTACAAATAGGTTAGATGTATTAGATGATGCATTATTAAGACCTGGAAGATTTGATAGACATATTCAAATCAATCTCCCTGATATTAAAGAAAGGGAAGGGATTTTAAAAGTTCATGCTGAAAATAAAAATCTCTCTTCTAAGATAAGTCTTTTAGATGTTGCTAAGAGAACTCCTGGGTTTTCAGGTGCTCAATTAGAAAATGTTATCAATGAAGCTACATTGTTAGCAGTTAGAGACAACCGTACCACAATTAACATTAATGACATTGATGAAGCAATTGATAGAGTAATAGCTGGTCCTGCTAAAAAGTCACGTGTAATTAGTGATGAAGATAGAAAACTAGTTGCTTATCATGAGGCTGGTCATGCCTTGGTTGGTTTACATGTCCACAGTAATGATGAAGTACAAAAGATTACCATTATTCCTCGTGGTCAAGCAGGGGGTTACACACTTTCAACACCTAAGAGTGGTGATCTTAACCTAAAAAGAAAATCTGATTTACTTGCAATGATAGCAACTGCTATGGGCGGTAGAGCTGCTGAAGAGGAAATCTATGGTAATTTAGAAATTACTACTGGCGCTTCTAGCGATTTTTATAAAGCAACTAATATTGCAAGAGCAATGGTAACCCAGCTTGGGATGTCTAAATTAGGTCAAGTGCAATATGTACCAAGTCAAGGGACACTCCCTTCTAATGTAAAACTTTATTCAGAACAAACTGCTAAAGATATTGACAATGAGATTAATTTCATTATTGAAGAACAGTATAAGAAAGCAAAAACAATCATTAAGAGTAACCGTAAGGAACTAGAATTGCTTGTAGAAGCACTTTTAATTGCTGAAACTATTTTGAAAAGTGATATTGACTTCATCCATAAAAACACTAAACTACCACCAGAAATCTTATTGCAAAAGCAAGAACAACAAGCAAAGCAAAAACTAAATAAATCTGAAGTAAAACCAGAAAGTGAAACAAACAGTTAG", "aa": "MKFTNIAGLQEEKHELLEIVDYLKNPLKYAQMGARSPRGVILYGPPGTGKTLLAKAVAGEAGVPFFQSTGSGFEDMLVGVGAKRVRDLFNKAKKAAPCIIFIDEIDSVGSKRGRVELSSYSVVEQTLNQLLAEMDGFTSRTGVVVMAATNRLDVLDDALLRPGRFDRHIQINLPDIKEREGILKVHAENKNLSSKISLLDVAKRTPGFSGAQLENVINEATLLAVRDNRTTININDIDEAIDRVIAGPAKKSRVISDEDRKLVAYHEAGHALVGLHVHSNDEVQKITIIPRGQAGGYTLSTPKSGDLNLKRKSDLLAMIATAMGGRAAEEEIYGNLEITTGASSDFYKATNIARAMVTQLGMSKLGQVQYVPSQGTLPSNVKLYSEQTAKDIDNEINFIIEEQYKKAKTIIKSNRKELELLVEALLIAETILKSDIDFIHKNTKLPPEILLQKQEQQAKQKLNKSEVKPESETNS", "aa_hexdigest": "a4de579dd6ef6f0a905f228af8bba544", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A929X996", "query_cov": 0.9852631578947368, "subject_cov": 0.8283185840707965, "identity": 0.551, "score": 470.0, "evalue": 9.03e-159, "valid": false, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "uniref50_id": "UniRef50_A0A7V6LLW0", "go_ids": [ "GO:0004222", "GO:0006508", "GO:0016020", "GO:0016887", "GO:0051301" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7V6LLW0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V6LLW0" ], "product": "ATP-dependent zinc metalloprotease FtsH" }, "genes": [ "ftsH" ], "id": "JPCNHPFPBM_1018", "locus": "GCA000292505_01018" }, { "type": "cds", "sequence": "contig_1", "start": 561502, "stop": 561660, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGAATGGTGGTTCTAGTAATAACACAAAGAGTATTGCAGGTTTTGAAACCCTTGGTACTTTCATTGCTCCTGATACTAGAGCTAGAGATGTATTAAATGGTTTGTTTGGCTTGCTACCAATTATTATCTTTGTAGCTTTCTTTTTACTCTTTTGA", "aa": "MMNGGSSNNTKSIAGFETLGTFIAPDTRARDVLNGLFGLLPIIIFVAFFLLF", "aa_hexdigest": "961a9bd2ce46309d40e9d0b999b1ff17", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5609.517299999995, "isoelectric_point": 5.87689037322998 }, "id": "JPCNHPFPBM_1019", "locus": "GCA000292505_01019" }, { "type": "cds", "sequence": "contig_1", "start": 562087, "stop": 562245, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTTGGTTCAAGGCTTTTATTGCACTTTTAAGTAAACATAAAAAGATAATTTTCGTCACTTTATTAACACTTATTAGTTTCATTACCATTGGCAGTATAATTACAGTTAATGTAGTTCAAACCAAAAAAACCCAAATCGGTCAAAAACTAACATAG", "aa": "MRWFKAFIALLSKHKKIIFVTLLTLISFITIGSIITVNVVQTKKTQIGQKLT", "aa_hexdigest": "4d3a29634f9a271594a38a2b92c049f1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5932.243300000001, "isoelectric_point": 11.471713829040528 }, "id": "JPCNHPFPBM_1020", "locus": "GCA000292505_01020" }, { "type": "cds", "sequence": "contig_1", "start": 562264, "stop": 562791, "strand": "-", "frame": 1, "gene": "hpt", "product": "hypoxanthine phosphoribosyltransferase", "db_xrefs": [ "COG:COG0634", "COG:F", "EC:2.4.2.8", "GO:0000166", "GO:0000287", "GO:0004422", "GO:0005829", "GO:0006166", "GO:0006178", "GO:0032263", "GO:0032264", "GO:0046100", "GO:0052657", "KEGG:K00760", "RefSeq:WP_009885573.1", "SO:0001217", "UniParc:UPI000012CB64", "UniRef:UniRef100_P47696", "UniRef:UniRef50_P47696", "UniRef:UniRef90_P47696" ], "nt": "ATGGGTATTAAATCTATTGTTATTAATGAACAACAGATAGAAGAAGGCTGTCAAAAAGCAGTTAATTGGTGCAATGCTAAATTTAATAATAAAAAGGTAATTGTTCTTGGCATTCTAAAAGGTTGCATCCCTTTCCTTGGCAAAGTGATAAGTAAATTTAGTTTTGACCTCCAACTAGATTTTATGGCAGTTGCTTCTTATCATGGTTCACATGTACAAAAACAACCACCTAAGATTGTGCTTGATATGTCCCATGACCCTAAAGATAAAGACATCCTTTTAATAGAAGATATTGTTGATAGTGGTAGATCTATTAAATTAGTTATTGATCTTCTAAAAACAAGGCATGCTAAATCAATAACTTTAATTAGCTTAATTGAAAAGATTAAACCCAAAGCCTTTGATATTAATATTGATTTTTCTTGTTTTAAAGTAAAAGATAATTTTTTGGTTGGCTTTGGTCTTGACTATGATGGTTTTTATCGTAACCTACCTTATGTTGGTGTGTTTGAACCAGACAATCCCTAA", "aa": "MGIKSIVINEQQIEEGCQKAVNWCNAKFNNKKVIVLGILKGCIPFLGKVISKFSFDLQLDFMAVASYHGSHVQKQPPKIVLDMSHDPKDKDILLIEDIVDSGRSIKLVIDLLKTRHAKSITLISLIEKIKPKAFDINIDFSCFKVKDNFLVGFGLDYDGFYRNLPYVGVFEPDNP", "aa_hexdigest": "4242e5c1e66cb0cecb4953ec06aac97f", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012CB64", "ncbi_nrp_id": "WP_009885573.1", "uniref100_id": "UniRef100_P47696", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012CB64", "RefSeq:WP_009885573.1", "UniRef:UniRef100_P47696" ] }, "ips": { "uniref100_id": "UniRef100_P47696", "uniref90_id": "UniRef90_P47696" }, "psc": { "uniref90_id": "UniRef90_P47696", "gene": "hpt", "product": "hypoxanthine phosphoribosyltransferase", "ec_ids": [ "2.4.2.8" ], "uniref50_id": "UniRef50_P47696", "cog_id": "COG0634", "cog_category": "F", "kegg_orthology_id": "K00760", "go_ids": [ "GO:0000166", "GO:0000287", "GO:0004422", "GO:0005829", "GO:0006166", "GO:0006178", "GO:0032263", "GO:0032264", "GO:0046100", "GO:0052657" ] }, "pscc": { "uniref50_id": "UniRef50_P47696", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47696" ], "product": "Hypoxanthine-guanine phosphoribosyltransferase" }, "genes": [ "hpt" ], "id": "JPCNHPFPBM_1021", "locus": "GCA000292505_01021" }, { "type": "cds", "sequence": "contig_1", "start": 562994, "stop": 563302, "strand": "-", "frame": 3, "gene": "ispF", "product": "putative protein MG459", "db_xrefs": [ "COG:COG0245", "COG:I", "GO:0008685", "GO:0016114", "SO:0001217", "UniRef:UniRef50_Q49436", "UniRef:UniRef90_Q49436" ], "nt": "ATGCAGTTAAGAGTTGGCCTAGGAAGCAAAAAATACCACATAAAATTAAGGAAAGAAAGTAAAAATAAATTTTGGTTAGGCGGTGTTGATTTTGAGAATTCTAAATATATTTATGATCTTGAAAACAGTGATGAAGTTAGTGATGTTATCCAATTAGCAGTTGCTGATGCTTTATTTGGTGCAACTGCATTAGGTGATGGTCATATTGTTTTCAATAAAGAAAAATCAACAGTCCAATTTAAAGGTACTGCAAAAAAAGAAGCACCAAGAGTCTTAGCAAGAACTTACAATTTCATCAGAAAAAACTGA", "aa": "MQLRVGLGSKKYHIKLRKESKNKFWLGGVDFENSKYIYDLENSDEVSDVIQLAVADALFGATALGDGHIVFNKEKSTVQFKGTAKKEAPRVLARTYNFIRKN", "aa_hexdigest": "56d3e83cfeccc1fa6a186aecde3bae45", "start_type": "ATG", "rbs_motif": null, "genes": [ "ispF" ], "truncated": "5-prime", "pseudogene": { "start": 562793, "stop": 563302, "observations": { "mutation": [ 562996 ], "stop": [ 562996 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q49436", "query_cov": 1.0, "subject_cov": 0.6035502958579881, "identity": 0.99, "score": 199.0, "evalue": 1.14e-63, "gene_start": 1, "gene_end": 102, "reference_start": 1, "reference_end": 102, "reference_sequence": "MQLRVGLGSKKYHIKLRKESKNKFWLGGVDFENSKYIYDLENSDEVSDVIQLAVADALFGATALGDGHIVFNKGKSTVQFKGTAKKEAPRVLARTYNFIRKNWIINNIDISLEIPQEQKVDDYKHAIFAFICSALRISELTINLKVRKPLDSNEINCLAVVLVERQKTK" }, "paralog": false, "identity": 0.9881656804733728, "score": 327.0, "evalue": 6.83e-117, "description": "Internal stop codon at 562996. Nonsense mutation around 562996.", "psc": { "uniref90_id": "UniRef90_Q49436", "gene": "IspF", "product": "Uncharacterized protein MG459", "uniref50_id": "UniRef50_Q49436", "cog_id": "COG0245", "cog_category": "I", "go_ids": [ "GO:0008685", "GO:0016114" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q49436", "UniRef:UniRef50_Q49436", "COG:COG0245", "COG:I", "GO:0008685", "GO:0016114" ] }, "pscc": { "uniref50_id": "UniRef50_Q49436", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49436" ], "product": "Uncharacterized protein MG459" } }, "id": "JPCNHPFPBM_1022", "locus": "GCA000292505_01022" }, { "type": "cds", "sequence": "contig_1", "start": 563475, "stop": 564413, "strand": "+", "frame": 3, "gene": "ldh", "product": "L-lactate dehydrogenase", "db_xrefs": [ "COG:C", "COG:COG0039", "EC:1.1.1.27", "GO:0004459", "GO:0005737", "GO:0006089", "GO:0006090", "GO:0006096", "KEGG:K00016", "RefSeq:WP_009885571.1", "SO:0001217", "UniParc:UPI000012E33D", "UniRef:UniRef100_P47698", "UniRef:UniRef50_P78007", "UniRef:UniRef90_P47698" ], "nt": "ATGAAAGGACCTAAAATTGCAATAGTAGGATCAGGTGCTGTTGGTACTAGTTTTCTATATGCTGCAATGACTCGTGCACTTGGTAGTGAATACATGATTATAGATATCAATGAAAAAGCTAAAGTTGGTAATGTATTTGATCTTCAAGATGCTTCCTCATCATGCCCAAATTTTGGCAAAGTAGTTGCTGGAGAATACAGTCAATTAAAAGATTATGATTTTATTTTCATTAGTGCAGGAAGACCTCAAAAACAAGGTGGGGAAACTAGGTTGCAGCTACTTGAAGGCAATGTAGAAATTATGAAAAGCATTGCCAAAGAAATCAAAAAATCTGGTTTTAATGGAGTTACATTAATCGCTTCAAACCCAGTTGATATCATGTCATATACATATCTTAAAGTTACTGGATTTGAACCTAATAAAGTAATTGGCTCTGGTACTTTACTTGATAGTGCAAGATTAAGATATGCAATTGCAACTAAGTACCAAATGAGTTCAAAGGATGTACAAGCATATGTTATTGGTGAACATGGTGATAGTTCAGTAAGTATTATTTCATCAGCTAAAATTGCAGGACTATCACTAAAACATTTTTCTAAAGCTAGTGATATTGAAAAAGAATTTGGTGAAATTGACCAATTTATCAGACGCAGAGCTTATGAAATTATTGAACGCAAAGGTGCTACTTTCTATGGAATTGGTGAAGCTAGTGCTGATGTAGCTGAACAAATTTTGAAAGATACTAAAGAAGTTAGAGTAGTGGCTCCTTTACTTACTGGTCAGTATGGAGCGAAGGATATGATGTTTGGAACTCCTTGTGTACTTTCAAGAAAAGGTATTGAAAAGATCTTGGAAATAGAACTTTCAAATACTGAAAAAGTTGCGCTTGAAAATTCAATTAAAGTTTTGAAAGACAACATTAAACTAGCAAAGCTTTAG", "aa": "MKGPKIAIVGSGAVGTSFLYAAMTRALGSEYMIIDINEKAKVGNVFDLQDASSSCPNFGKVVAGEYSQLKDYDFIFISAGRPQKQGGETRLQLLEGNVEIMKSIAKEIKKSGFNGVTLIASNPVDIMSYTYLKVTGFEPNKVIGSGTLLDSARLRYAIATKYQMSSKDVQAYVIGEHGDSSVSIISSAKIAGLSLKHFSKASDIEKEFGEIDQFIRRRAYEIIERKGATFYGIGEASADVAEQILKDTKEVRVVAPLLTGQYGAKDMMFGTPCVLSRKGIEKILEIELSNTEKVALENSIKVLKDNIKLAKL", "aa_hexdigest": "99940a250ddf1c25b4687a3ef06d9c6e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012E33D", "ncbi_nrp_id": "WP_009885571.1", "uniref100_id": "UniRef100_P47698", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012E33D", "RefSeq:WP_009885571.1", "UniRef:UniRef100_P47698" ] }, "ips": { "uniref100_id": "UniRef100_P47698", "uniref90_id": "UniRef90_P47698" }, "psc": { "uniref90_id": "UniRef90_P47698", "gene": "ldh", "product": "L-lactate dehydrogenase", "ec_ids": [ "1.1.1.27" ], "uniref50_id": "UniRef50_P78007", "cog_id": "COG0039", "cog_category": "C", "kegg_orthology_id": "K00016", "go_ids": [ "GO:0004459", "GO:0005737", "GO:0006089", "GO:0006090", "GO:0006096" ] }, "pscc": { "uniref50_id": "UniRef50_P78007", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78007" ], "product": "L-lactate dehydrogenase" }, "genes": [ "ldh" ], "id": "JPCNHPFPBM_1023", "locus": "GCA000292505_01023" }, { "type": "cds", "sequence": "contig_1", "start": 564557, "stop": 564826, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAAAAAAAGTTTATTAACTTGTTTTGTGAAGTTAGATGATTATGTTTTTCACTCATTTTTAGTTAATACATTTGAAACAACTAAAGATGCAATATTAAAAACCTTACTAGATAGTTATCTTAACACTCTGAAATTTCAAGTAAAGTTTTATGAAAGTTGTGAAAAACGTGATCTTGATTTTGAATTAAAAGTGAAAGAATATCAAACTCCTAGTTATTTCATTACTAAATTCAATAACCAATTTAAGGGTTTTTATGAAGGATGA", "aa": "MFKKSLLTCFVKLDDYVFHSFLVNTFETTKDAILKTLLDSYLNTLKFQVKFYESCEKRDLDFELKVKEYQTPSYFITKFNNQFKGFYEG", "aa_hexdigest": "bc75fa5df9c9026d37728f81e0de5c91", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10731.238099999993, "isoelectric_point": 7.705798530578614 }, "id": "JPCNHPFPBM_1024", "locus": "GCA000292505_01024" }, { "type": "cds", "sequence": "contig_1", "start": 564929, "stop": 565687, "strand": "-", "frame": 3, "gene": "ydhJ", "product": "putative protein MG461", "db_xrefs": [ "COG:COG1078", "COG:R", "GO:0006203", "GO:0008832", "SO:0001217", "UniRef:UniRef50_P47699", "UniRef:UniRef90_P47699" ], "nt": "ATGCAACAAACCTTTTTTAAAGATCCTATTTTAGGAGAAATTATTTTTGATGAAAACACAAAGTGGATGTATGAATTGGTCAATACAAAAGCTTTTCAAAGGTTAAGAAACATCAAACAATTAGGAATTAACTTCCATTTTTATCCTAGTGGAGTTCACACTAGGTACGCCCATTCTTTAGGAGTTTATGAACTGATAAGAAGGATCTTAAATAGTTCTGCATTTTTAAATATTGATCAAATTAAAAAACAAACTGTTTTAGTAGCTGGTTTGTTGCATGATCTTGGTCATGGACCTCATTCTCATGCTTTTGAAATCTATTTTGCTAAAAATCCTGATTTTAAAAAACAACTTTTTATTCATGAAAAGGTTACTTCAATGTTAGTTAATAGTGAACCTATTGTCTCTATCTTAAAAGCTAATAAGATAGATCCAAATCTGATTGGCGCTTTAATTGACGAAAATCAAAATATTCAACCTATTAACTGGTGGATGAGACAGCTAATATCTTCAGATCTTGACACAGACAGAATGGATTATTTATTACGTGATGCTTATTTTACAGGTACCAGTCATTCATTAGTTGATTATCAATCAATTATCAATGGAATGGAATGTGTAGATAATCAGGGCACTTATGAAATTGTTTTTCAAGAAAAATGCTTACCTTTCATTGAAAACTTCTTGATAACAAGACACCACATGTATCAATCAATCTACAGTGATGGTAGAAGTATTGCTACAGAACTTAACTTATGA", "aa": "MQQTFFKDPILGEIIFDENTKWMYELVNTKAFQRLRNIKQLGINFHFYPSGVHTRYAHSLGVYELIRRILNSSAFLNIDQIKKQTVLVAGLLHDLGHGPHSHAFEIYFAKNPDFKKQLFIHEKVTSMLVNSEPIVSILKANKIDPNLIGALIDENQNIQPINWWMRQLISSDLDTDRMDYLLRDAYFTGTSHSLVDYQSIINGMECVDNQGTYEIVFQEKCLPFIENFLITRHHMYQSIYSDGRSIATELNL", "aa_hexdigest": "41720e1b1bc383fcce95409d307405d8", "start_type": "ATG", "rbs_motif": null, "genes": [ "ydhJ" ], "truncated": "5-prime", "pseudogene": { "start": 564626, "stop": 565687, "observations": { "mutation": [ 564931 ], "stop": [ 564931 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47699", "query_cov": 1.0, "subject_cov": 0.5929411764705882, "identity": 1.0, "score": 515.0, "evalue": 2.59e-182, "gene_start": 1, "gene_end": 252, "reference_start": 1, "reference_end": 252, "reference_sequence": "MQQTFFKDPILGEIIFDENTKWMYELVNTKAFQRLRNIKQLGINFHFYPSGVHTRYAHSLGVYELIRRILNSSAFLNIDQIKKQTVLVAGLLHDLGHGPHSHAFEIYFAKNPDFKKQLFIHEKVTSMLVNSEPIVSILKANKIDPNLIGALIDENQNIQPINWWMRQLISSDLDTDRMDYLLRDAYFTGTSHSLVDYQSIINGMECVDNQGTYEIVFQEKCLPFIENFLITRHHMYQSIYSDGRSIATELNLWFVFQRIKALIEEDNFNFHNFKNVESVIKPLLKNQLFKKSLLTCFVKLDDYVFHSFLVNTFETTKDAILKTLLDSYLNTLKFQVKFYESCEKRDLDFELKVKEYQTPSYFITKFNNQFKGFYEGWNKHKNELKIKTSQNKIKNLSEISMLVKRSNELFFENSFYRWANVFYQN" }, "paralog": false, "identity": 0.9971671388101983, "score": 712.0, "evalue": 2.15e-262, "description": "Internal stop codon at 564931. Nonsense mutation around 564931.", "psc": { "uniref90_id": "UniRef90_P47699", "gene": "YdhJ", "product": "Uncharacterized protein MG461", "uniref50_id": "UniRef50_P47699", "cog_id": "COG1078", "cog_category": "R", "go_ids": [ "GO:0008832", "GO:0006203" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47699", "UniRef:UniRef50_P47699", "COG:COG1078", "COG:R", "GO:0008832", "GO:0006203" ] }, "pscc": { "uniref50_id": "UniRef50_P47699", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47699" ], "product": "Uncharacterized protein MG461" } }, "id": "JPCNHPFPBM_1025", "locus": "GCA000292505_01025" }, { "type": "cds", "sequence": "contig_1", "start": 565668, "stop": 565826, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF19269.4" ], "nt": "GTGTTTAACTTAAAAGGTAAACAACTTTTTATGCCAATAAGGTTAATTTTTACAAATAAGGAGCATGGACCTGATTTAGCACATATTATTGAAATTTTTGATAAAGAAAGTGCAATAAACTTAATTAAGCAATTTATTAATGCAACAAACCTTTTTTAA", "aa": "MFNLKGKQLFMPIRLIFTNKEHGPDLAHIIEIFDKESAINLIKQFINATNLF", "aa_hexdigest": "df98cf08eec54401ee998c3ec3e4ea52", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF19269.4", "name": "Anticodon_2", "length": 43, "aa_cov": 0.8269230769230769, "hmm_cov": 1.0, "evalue": 2.6629295289537697e-08, "score": 29.451568603515625, "start": 3, "stop": 45 } ], "seq_stats": { "molecular_weight": 6074.122299999998, "isoelectric_point": 8.181634330749514 }, "id": "JPCNHPFPBM_1026", "locus": "GCA000292505_01026" }, { "type": "cds", "sequence": "contig_1", "start": 565869, "stop": 566090, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATGTTAAGGTTGATTATTTAAAAGATAAAAACAGGGAAATAAGTTTACTTTTTAAAAATCAAATAACCCATGGTGTTCAAATAAACGAATTGATAAGAGAATCTTTTGCCACTAAAATAGGTGTTGAAAACTTAGCTAAGAAAAGTCATATTTTGTTTAAAAACATCAAACTTTTTTTAGAACAGCTTGCCAAATCTTTACAAGGGTTGGAAGAATGA", "aa": "MDVKVDYLKDKNREISLLFKNQITHGVQINELIRESFATKIGVENLAKKSHILFKNIKLFLEQLAKSLQGLEE", "aa_hexdigest": "4d338d3cba2ef8fdbfe49036ece3fb04", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8469.808099999997, "isoelectric_point": 9.004702186584474 }, "id": "JPCNHPFPBM_1027", "locus": "GCA000292505_01027" }, { "type": "cds", "sequence": "contig_1", "start": 566265, "stop": 566507, "strand": "-", "frame": 2, "gene": null, "product": "glutamate--tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003594285F" ], "nt": "ATGGAAATTACTGATGTTTTAAGGGGAGCTGAGCACATCTCTAACACTGCATACCAACTTGCTATATATCAAGCATTAGGTTTTAAAAGAATTCCCCGCTTTGGTCATCTTTCAGTTATTGTTGATGAAAGTGGCAAAAAACTTTCTAAACGTGATGAGAAAACTACTCAGTTTATTGAGCAGTTTAAACAACAAGGCTATCTACCTGAAGCATTATTAAATTTCTTAGCACTCTTAGGTTGA", "aa": "MEITDVLRGAEHISNTAYQLAIYQALGFKRIPRFGHLSVIVDESGKKLSKRDEKTTQFIEQFKQQGYLPEALLNFLALLG", "aa_hexdigest": "648d89fc9c6321a5d78c0a33260bc56c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003594285F", "query_cov": 0.875, "subject_cov": 0.8235294117647058, "identity": 0.6, "score": 85.9, "evalue": 2.12e-20, "valid": false, "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_UPI003594285F" }, "pscc": { "uniref50_id": "UniRef50_UPI003594285F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003594285F" ], "product": "glutamate--tRNA ligase family protein" }, "genes": [], "id": "JPCNHPFPBM_1028", "locus": "GCA000292505_01028" }, { "type": "cds", "sequence": "contig_1", "start": 566625, "stop": 567122, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00749.27" ], "nt": "ATGGAAAAAATTAGAACACGTTATGCACCATCCCCAACAGGATATCTGCATGTTGGTGGTACAAGAACAGCAATCTTTAACTTTTTACTAGCCAAGCACTTTAATGGTGAGTTTATTATCAGGATAGAAGATACTGATACTGAAAGAAACATAAAAGAAGGAATTAATTCACAATTTGATAACTTGCGTTGGCTTGGAGTCATTGCAGATGAATCGGTTTATAACCCTGGCAATTATGGTCCATATCTGCAATCACAAAAACTAGCAGTTTATAAAAAACTAGCATTTGATTTAATTGAAAAAAATCTGGCATATCGTTGCTTCTGTAGCAAAGAAAAATTAGAGTCAGATAGAAAACAAGCCATTAATAACCACAAAACCCCTAAATACTTAGGTCATTGTCGTAATTTACATTCCAAGAAAATTACTAATCACTTAGAAAAAAATGATCCTTTTACTATCCGCTTAAAAATAAACAATGAAGCTGAATATAGTTGA", "aa": "MEKIRTRYAPSPTGYLHVGGTRTAIFNFLLAKHFNGEFIIRIEDTDTERNIKEGINSQFDNLRWLGVIADESVYNPGNYGPYLQSQKLAVYKKLAFDLIEKNLAYRCFCSKEKLESDRKQAINNHKTPKYLGHCRNLHSKKITNHLEKNDPFTIRLKINNEAEYS", "aa_hexdigest": "a1bd8d8224c3b4a54fc6b7dc7310f2bc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00749.27", "name": "tRNA-synt_1c", "length": 163, "aa_cov": 0.9757575757575757, "hmm_cov": 0.950920245398773, "evalue": 1.2612422676375798e-54, "score": 180.3483428955078, "start": 3, "stop": 163 } ], "seq_stats": { "molecular_weight": 19212.628600000015, "isoelectric_point": 9.282238578796388 }, "id": "JPCNHPFPBM_1029", "locus": "GCA000292505_01029" }, { "type": "cds", "sequence": "contig_1", "start": 567109, "stop": 567888, "strand": "-", "frame": 1, "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "db_xrefs": [ "COG:COG0030", "COG:J", "EC:2.1.1.182", "GO:0000179", "GO:0003723", "GO:0005829", "GO:0006364", "GO:0031167", "GO:0052908", "RefSeq:WP_010869490.1", "SO:0001217", "UniParc:UPI000012E0AD", "UniRef:UniRef100_P47701", "UniRef:UniRef50_P47701", "UniRef:UniRef90_P47701" ], "nt": "GTGAATAGTTTTTTTCCTTCACGTAAATTAGGTCAAAATTTTACGGTTAATTTAAGCGTCATTAAAAGAATTTTTGCTTTTGTTAAAAATTTAAATCCACAAGCAATTGTTGAAATAGGTGTTGGTAAAGGAGCGTTAACAAATTATTTGTTAAAACTCAAAATACCTTACAAGGGGATAGAAATTGATAAACGCTTAATTGAATATCTTCTAGTTGAAAAGATATTAACTGAAGACCAACTAGTTAAAGGCGATATTCTCAAAAAGGACTTTAATAGTTTTTTTGAAAATTTAAGTCCATTATTGTGTGGTAATATCCCATATAGCATTACATCTCCAATAATCAATAAGTTTTTAGAATCAAAGCTTCGAAGCTTTGTTTTAATGACACAAAAAGAGTTTGCTAATCGGCTTCTGGCAAAGGTTAATTCTAGTGATTATAGTGCCTTTGGTGCTTTTTGTCAATACTATTTGACTATTACAACAGTTTTTAAAATTGATAGGCATGCTTTTAAACCTAAACCTAAGGTGGATTCAACCTTAATATTATTAGAAAAAAATAAATCGGTTAGTTATGACTTTAAGTTTGGTCTGTTTTTAAAGCAATGTTTCAATCAAAGAAGGAAGATGTTAATTAATAACTTAAAGCATTTTTTTGCAGTTGATTATTTACTTAACATTATTCAAAAGCAAAATTTAAAGACTAGCATTAGAGCGCAAGAGTTGAGTCCTTGTCAACTCTTTAATCTTTACCAAAACATTTGTAATGGAAAAAATTAG", "aa": "MNSFFPSRKLGQNFTVNLSVIKRIFAFVKNLNPQAIVEIGVGKGALTNYLLKLKIPYKGIEIDKRLIEYLLVEKILTEDQLVKGDILKKDFNSFFENLSPLLCGNIPYSITSPIINKFLESKLRSFVLMTQKEFANRLLAKVNSSDYSAFGAFCQYYLTITTVFKIDRHAFKPKPKVDSTLILLEKNKSVSYDFKFGLFLKQCFNQRRKMLINNLKHFFAVDYLLNIIQKQNLKTSIRAQELSPCQLFNLYQNICNGKN", "aa_hexdigest": "54b49b755d41550070f0724426524017", "start_type": "GTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000012E0AD", "ncbi_nrp_id": "WP_010869490.1", "uniref100_id": "UniRef100_P47701", "db_xrefs": [ "SO:0001217", "UniParc:UPI000012E0AD", "RefSeq:WP_010869490.1", "UniRef:UniRef100_P47701" ] }, "ips": { "uniref100_id": "UniRef100_P47701", "uniref90_id": "UniRef90_P47701" }, "psc": { "uniref90_id": "UniRef90_P47701", "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "ec_ids": [ "2.1.1.182" ], "uniref50_id": "UniRef50_P47701", "cog_id": "COG0030", "cog_category": "J", "go_ids": [ "GO:0000179", "GO:0003723", "GO:0005829", "GO:0006364", "GO:0031167", "GO:0052908" ] }, "pscc": { "uniref50_id": "UniRef50_P47701", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47701" ], "product": "Ribosomal RNA small subunit methyltransferase A" }, "genes": [ "rsmA" ], "id": "JPCNHPFPBM_1030", "locus": "GCA000292505_01030" }, { "type": "cds", "sequence": "contig_1", "start": 567881, "stop": 568195, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATCGTTTTACCAGTCCAATTTCTCTCTCAAAAACTTCCTCAAGTGTGGGCAAGTAAGCGTAATGAGAATGCGAAAGCCCATTCTCAAAAAAGTATTGAACAACTCAACAAAACTAAAAAGATGCAATTAATTTTTTACTTTGTGTTTGCTGCTATTACTGCATTTAGTGCTGCTGGAGTTGGTGTTTATTGGTTTTTAAATGCTTTGTTTACTTTATTACAATCTTATTTAACCCATGTTTTCATTGTCAAAAGAAGGGAAAAAAGAAAGCAAAATTATTCCAAATTAGATTTGATTTTAGAGCGTGAATAG", "aa": "MIVLPVQFLSQKLPQVWASKRNENAKAHSQKSIEQLNKTKKMQLIFYFVFAAITAFSAAGVGVYWFLNALFTLLQSYLTHVFIVKRREKRKQNYSKLDLILERE", "aa_hexdigest": "3b9395dc01ddeff6ab975b8eff9e27a7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12209.249899999986, "isoelectric_point": 10.167453956604003 }, "id": "JPCNHPFPBM_1031", "locus": "GCA000292505_01031" }, { "type": "cds", "sequence": "contig_1", "start": 568214, "stop": 568642, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGCAACCAGAGTTCCACTAGGTAGCGGGGTGGAACTTGGGTTTAACATGATTCTTTCATTAATAGTACTTTTACTATTAGTTCGCTTGATAACTATTGTGATTACCTTAAACTCTACTTTAGCACTTGAAAAGATGAATGAAGTGCAAGGTAAATTAGCAGAAATTAACGCTAAATATAAAGGGGCAATAGATTTACAAAGTAAAAGAAACAGACAGTTAGAGATAATGTCACTTTATAAAAAGCACAACATTAAATCATCTGCTGCTTTTGTTCAAGTTTTTGTAACTTTACCTATCTTTTTAATTATTTATAGGATTGTTACTACTTTAAGACCAATTAAAGCAATCATCTTATTTAACTTTTGGGATCTTTCAAAAGTACCTTTAACAGAAATTTTCTCTAATTTCACTACAACTGGTTGA", "aa": "MYATRVPLGSGVELGFNMILSLIVLLLLVRLITIVITLNSTLALEKMNEVQGKLAEINAKYKGAIDLQSKRNRQLEIMSLYKKHNIKSSAAFVQVFVTLPIFLIIYRIVTTLRPIKAIILFNFWDLSKVPLTEIFSNFTTTG", "aa_hexdigest": "4e3e1113c7868ad5bacbfa31eb30f007", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16044.083099999989, "isoelectric_point": 9.992873573303225 }, "id": "JPCNHPFPBM_1032", "locus": "GCA000292505_01032" }, { "type": "cds", "sequence": "contig_1", "start": 569365, "stop": 569511, "strand": "-", "frame": 1, "gene": "rpmH", "product": "50S ribosomal protein L34", "db_xrefs": [ "COG:COG0230", "COG:J", "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:1990904", "KEGG:K02914", "RefSeq:WP_009885567.1", "SO:0001217", "UniParc:UPI0000133FB9", "UniRef:UniRef100_P47704", "UniRef:UniRef50_P0A7P5", "UniRef:UniRef90_P47704" ], "nt": "ATGAAACGAACATACCAACCAAGCAAATTAAAGCGTGCTAAAACCCATGGTTTTATGGCTAGGATGGCAACTGCACAAGGACGTAAAGTTTTAAGGCAAAGACGTTTTAAAAATCGTGCTCAACTCACGGTTTCCAGTGAGCGTTAA", "aa": "MKRTYQPSKLKRAKTHGFMARMATAQGRKVLRQRRFKNRAQLTVSSER", "aa_hexdigest": "b7a51c6f33bc053523970850e910c399", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0000133FB9", "ncbi_nrp_id": "WP_009885567.1", "uniref100_id": "UniRef100_P47704", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000133FB9", "RefSeq:WP_009885567.1", "UniRef:UniRef100_P47704" ] }, "ips": { "uniref100_id": "UniRef100_P47704", "uniref90_id": "UniRef90_P47704" }, "psc": { "uniref90_id": "UniRef90_P47704", "gene": "rpmH", "product": "50S ribosomal protein L34", "uniref50_id": "UniRef50_P0A7P5", "cog_id": "COG0230", "cog_category": "J", "kegg_orthology_id": "K02914", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P0A7P5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0A7P5" ], "product": "Large ribosomal subunit protein bL34" }, "genes": [ "rpmH" ], "id": "JPCNHPFPBM_1033", "locus": "GCA000292505_01033" }, { "type": "cds", "sequence": "contig_1", "start": 569537, "stop": 569932, "strand": "-", "frame": 3, "gene": "lolD", "product": "Putative ABC transporter ATP-binding protein MG467", "db_xrefs": [ "COG:COG1136", "COG:M", "GO:0005524", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P75110", "UniRef:UniRef90_P47705" ], "nt": "TTGTTAAGTGCTTATGATAATATTGCCATTTCACAAAACTTAATTCCCAAATATCAAAGACGACTTGATATAGAAGAGTTGGCTGAAAAATTAGATATTAAAGAAATCTTGTATAAATTTCCTTATGAGATGTCAGGTGGGCAAAAACAAAGAGTGGCCATCGCCCGTGCTATTATTAAAGAACCTAAACTATTACTTTGTGATGAACCTACTGGTGCACTTGATAGTAATTCTGCGGAAAATATCATTAATTTATTACAAACAATTAACAAAACATATAAGCAAACCATCCTAATGGTTACCCATGATGAAAGCCTAACAAGGATAGCTAATAGAATCATTAAAATTTCAGATGGTAAAATAGTATCTAACCAATTAGTCAGACCCTTAGTTTAG", "aa": "MLSAYDNIAISQNLIPKYQRRLDIEELAEKLDIKEILYKFPYEMSGGQKQRVAIARAIIKEPKLLLCDEPTGALDSNSAENIINLLQTINKTYKQTILMVTHDESLTRIANRIIKISDGKIVSNQLVRPLV", "aa_hexdigest": "b8f44a73be943d5c3953f018733a964b", "start_type": "TTG", "rbs_motif": null, "genes": [ "lolD" ], "truncated": "3-prime", "pseudogene": { "start": 569537, "stop": 570232, "observations": { "mutation": [ 570205 ], "stop": [ 570205 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P47705", "query_cov": 1.0, "subject_cov": 0.4212218649517685, "identity": 0.985, "score": 248.0, "evalue": 8.6e-81, "gene_start": 1, "gene_end": 131, "reference_start": 181, "reference_end": 311, "reference_sequence": "MKKGNKTVWNECIDILDNVKSPSFSANFDDYFKKSKSKPPKKNKKVLNNIKKAELKLKKKANKKQKANTLYIPPFAQQAKGIVITINKMWKNVHNDDSKQEISILSDVSLQIAYGEIVIILGSSGSGKTTLLNLIGGYDSISLGSCIVANCPLEKCTSEQLLTYRKNNLGYVYQRYNLIELLSAYDNIAISQNLIPKYQRRLDIEELAEKLDIKEILYKFPYEMSGGQKQRVAIARAIIKEPKLLLCDEPTGALDSNSAENIINLLQTINKTYKQTILMVTHDVSLTRIANRIIKISDGKIVSNQLVRPLV" }, "paralog": false, "identity": 0.987012987012987, "score": 440.0, "evalue": 8.5e-159, "description": "Internal stop codon at 570205. Nonsense mutation around 570205.", "psc": { "uniref90_id": "UniRef90_P47705", "gene": "LolD", "product": "Putative ABC transporter ATP-binding protein MG467", "uniref50_id": "UniRef50_P75110", "cog_id": "COG1136", "cog_category": "M", "go_ids": [ "GO:0005524", "GO:0016887" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P47705", "UniRef:UniRef50_P75110", "COG:COG1136", "COG:M", "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P75110", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75110" ], "product": "Putative ABC transporter ATP-binding protein MG467 homolog" } }, "id": "JPCNHPFPBM_1034", "locus": "GCA000292505_01034" }, { "type": "cds", "sequence": "contig_1", "start": 570515, "stop": 570868, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATCAAGTAGATGCGTTTCTTTTAGGAACTATTATTCCTTTCATTTTCATTACTTGTGTTGTACTTGGTATCTCAATGTTAGAAGAGATGAAAAGAATCTTTATTTCACTTAAGGCTATTGGCTATAGAGATGTACAAAATTTAATTAGTTTACTTACCTTCTTTATCCCTGCATTTGTTTTAGCATTACTAATTTCTATTGGAGTTTTAGCTGGTGTTTTAATTGGTATCCAAGCAGTTGTTTTCAATGTTGCACAAGTCTTTTTAACTAATGTATTTGAGTTTTTGCCATACATGGTAGGGATAGTATTATTTGGTGTTACTATCTTTGTAATTGGTTCTTACTTTTGA", "aa": "MNQVDAFLLGTIIPFIFITCVVLGISMLEEMKRIFISLKAIGYRDVQNLISLLTFFIPAFVLALLISIGVLAGVLIGIQAVVFNVAQVFLTNVFEFLPYMVGIVLFGVTIFVIGSYF", "aa_hexdigest": "88d4140422110cf46f82bfa01e97becf", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12914.61039999999, "isoelectric_point": 4.935693550109861 }, "id": "JPCNHPFPBM_1035", "locus": "GCA000292505_01035" }, { "type": "cds", "sequence": "contig_1", "start": 571004, "stop": 572725, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAGTAAACCTGTTAATGTCTTAATTGATCAAGGCAATGAGACCAAGTTTTTAGTTCTTCTTCATCTTTTAAAACATAAAAGTCATACAATGTCACCCTTGACCAAGTTTAGGGTTTCATTAATCGTTTCACGTTTTTCACGTTTATTTACCTATGTAGGATTGAGTTCAGTTGCACTTTTATTAATTGGGATTGCAGGTACTATTCCCCAAAAATTTAGTGCAGCACAAACAAGCACTTCCTTAAATAGAAACTTTAACTATAAGTTGAATTTACAAACACCAACAGAGCAATCTGGATGGTATGCAATTCAACCCTATTCACACTTTGGAGTTACTGATAATAATAATGGTATTAAAACCTTGTATAACGAAAGTGTTCAAGCAAATTCTCAAAATGAGCACCCTTATAAACCAAGTAATTTAAAACTTAAAAACCGCCAGGATCAACCAATAAAAGCAGCAGATGGAACTGAGCTTGAATTGGGCAACTTACTTTTGCCTAGTTATGGTGGTGCTCAACAGTTAAACACTGATGAGAATTTCTTTAGGCATGCCTCTCTATCAAAATGGATTATTGACTTTCCTATTAGGGTTGGCGGTTCTAACATTAACCCTTGGGAAATTGTTGAAAAATCAATTCCTAAACAGATTACTCAGTTACTTTCAGCATCTAGTGATCAGTTTTTAATTTCAGTATTAACTGATGACTTTTTCAATAACTTAAATGCTAATGGGTTTTTAATTAGAAACCCTAGAACTAATCAAATTCAGTTAGATGCTAGTAGGGTACTAACTACAATTGATGTATTTAATCCTGGTGGGGTTAAATTTAATGATAGCTTTCTTAGTTTTATGTTGAAAGTTTATGGGGATTTTGAACTTGCAAAACAAGATTCTAAGTTAAATTTTGGGATTGTTCCTGTTGACCCTGCCATAGAAGAAACCTACACTTATGTTGAAGGCCCATTTGGTTTTCAAGAAGATAATCTTGATGAGAATTCACCATACACTTTAACAGGGATTAACCCTGAATCAAGTTTTGTTAATCTGATAGATGGTAGTGGTAACTCTTTAAGAAATTTAATTAGTTCTGATCAGGAAATGAATGTAATTGTTAATGCTGGTTTTCAATATGCTAACAACATTAATATTGGTGATTATGTTTATATAAAACCGAAAAATACTGCCACAAGATACTCTGAAAAATTTCTAAAAGCACCCTTAAATAATTCAACAGTAGCATTTAAAGTTGTTGGTGTTTCTACTGATGCTTTTGGACAAGAACTTTATATTAACCAACACATTGCTAATAACTTATTAAAACTAAGTGGTAATCAGGGCAGAGGTATTATTAGAGATGTTATTAAAAAGACAAATGGCCAATCTCAAAGTAGTGATGAATATGAAATTGATTATGTAAAGCCAAACGGTTATGTTCCTTTTAATGGTGTATTTTCCAAAGAATTAAAACCTTCTTTATTAAATAAGGCATTAGTATTAAACTCGATAATTGGTGTTTGGGGTAATTTCACTGACTTTGGTAATAATTTCCAAAATTTAGTAAGGAATAAACTTGATAAAGTTATTACATCCATACTACCTACTGATCCTGAAATTATTAATAAGTTAGCACAAGAAAAACAGATTATTAATACTACTTCAATGAATTATGAATCGCTTCGTAAAGAACTAGTTAATAAATACAAAACAGAATGA", "aa": "MFSKPVNVLIDQGNETKFLVLLHLLKHKSHTMSPLTKFRVSLIVSRFSRLFTYVGLSSVALLLIGIAGTIPQKFSAAQTSTSLNRNFNYKLNLQTPTEQSGWYAIQPYSHFGVTDNNNGIKTLYNESVQANSQNEHPYKPSNLKLKNRQDQPIKAADGTELELGNLLLPSYGGAQQLNTDENFFRHASLSKWIIDFPIRVGGSNINPWEIVEKSIPKQITQLLSASSDQFLISVLTDDFFNNLNANGFLIRNPRTNQIQLDASRVLTTIDVFNPGGVKFNDSFLSFMLKVYGDFELAKQDSKLNFGIVPVDPAIEETYTYVEGPFGFQEDNLDENSPYTLTGINPESSFVNLIDGSGNSLRNLISSDQEMNVIVNAGFQYANNINIGDYVYIKPKNTATRYSEKFLKAPLNNSTVAFKVVGVSTDAFGQELYINQHIANNLLKLSGNQGRGIIRDVIKKTNGQSQSSDEYEIDYVKPNGYVPFNGVFSKELKPSLLNKALVLNSIIGVWGNFTDFGNNFQNLVRNKLDKVITSILPTDPEIINKLAQEKQIINTTSMNYESLRKELVNKYKTE", "aa_hexdigest": "baaf41829995a5517beafec7b22b7356", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 64185.76100000007, "isoelectric_point": 7.075681495666504 }, "id": "JPCNHPFPBM_1036", "locus": "GCA000292505_01036" }, { "type": "cds", "sequence": "contig_1", "start": 572741, "stop": 573514, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02687.27" ], "nt": "TTGCAAACACCAAACAATTTCCCTAACTGGTTAAATGATCTACCTGATCGTTATAAATTTAGTATTAACGGACTAACATTTGCAATTTTAGGTATAGGTGAATCAGTAGAAACTGGTTACCCAGTGTTATCTTTACAATCTCCGTTGCCTAATACCCAAGATGAAGCGTTAATCTTTGTTAATGATCAAGCTTACAGAAGTATTCTGTTTGCTGTACCAGCAGCTAACCAAGAAAATTATTATGCTTTTAAGTCAACAGATTTAAAACAGCACACTGACCAAGACCCAGTGCAGTTTATTGCTAATAGATTAGAAGGATATCTAGATGTTCCTAGAAGTGATTTAGCATTTAATGTTAAAGATATTTCTAAGTTTAATTATCTGACAACAGCAAGAAACTATTTTCCTGATTTAGTGCAAAGCTATTTGGCAATAGTAAGTACTGTTATTGCTATATTTTTGATTATTCTTGCTCTTTATTTAATTATTCTTTTAATTAAGTCATTTATTAAAAAGAACCAAACTGAGTTTTCTATTATCAGAGCAGGGGGATTTTCAACAACTAAATTTATTGTTGGAATGAGTGTGTTTGCTGGTATTGTTGCAATTGTTTCTAGTTTTTTAGGAGTTTTATTTGCCTTTCTTCTAGAAGGACAAGTAAAGGGAATCATAAATAGGTATTGGTTTATTGCTTTACCTGAAAATAGCTTTAACTGGTTATCGTTTTTTGGTTCTTTCTTTATAACCTTTTTTGTTTTTGAATTTATTAGTTGA", "aa": "MQTPNNFPNWLNDLPDRYKFSINGLTFAILGIGESVETGYPVLSLQSPLPNTQDEALIFVNDQAYRSILFAVPAANQENYYAFKSTDLKQHTDQDPVQFIANRLEGYLDVPRSDLAFNVKDISKFNYLTTARNYFPDLVQSYLAIVSTVIAIFLIILALYLIILLIKSFIKKNQTEFSIIRAGGFSTTKFIVGMSVFAGIVAIVSSFLGVLFAFLLEGQVKGIINRYWFIALPENSFNWLSFFGSFFITFFVFEFIS", "aa_hexdigest": "3a8d471eaf58702ca7def26266c0fc43", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02687.27", "name": "FtsX", "length": 108, "aa_cov": 0.42023346303501946, "hmm_cov": 0.9814814814814815, "evalue": 5.089580298019996e-11, "score": 37.818782806396484, "start": 149, "stop": 256 } ], "seq_stats": { "molecular_weight": 29132.26780000003, "isoelectric_point": 5.189138603210448 }, "id": "JPCNHPFPBM_1037", "locus": "GCA000292505_01037" }, { "type": "cds", "sequence": "contig_1", "start": 573524, "stop": 573694, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGAGATCCCTATTACTGTTAAAAATGGGGTCAATCCATTAATATTACCTACTGATCAACAAAATTTAGTTGTGCAAACCCCTGAAGCACACGGAGCGGTTGTTTCTCAACAATGGTTGTTTAGACACAATAAAGAAATTCTTCCCCAGGAAGGGGAATATGCTTGA", "aa": "MLEIPITVKNGVNPLILPTDQQNLVVQTPEAHGAVVSQQWLFRHNKEILPQEGEYA", "aa_hexdigest": "de0f0ed0d6112401acaecc8ac71561fe", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6305.134699999999, "isoelectric_point": 5.013165092468261 }, "id": "JPCNHPFPBM_1038", "locus": "GCA000292505_01038" }, { "type": "cds", "sequence": "contig_1", "start": 573833, "stop": 573982, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGCTAGTTTAAAAAATACCCAAGCTTTAAGTCCAAAATCTACTAAATTTCCCATTTATCCAAAGTTAGCAAATATTATTGCTCAAGCCCAATTACCAGAAGCTACTAATATCCCAACTACTGCTTTAGATGCTTTAAAACAATGA", "aa": "MTASLKNTQALSPKSTKFPIYPKLANIIAQAQLPEATNIPTTALDALKQ", "aa_hexdigest": "bcf5b4d866410aac7ef0c2d45bb43c5e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5253.076300000001, "isoelectric_point": 9.699606895446777 }, "id": "JPCNHPFPBM_1039", "locus": "GCA000292505_01039" }, { "type": "cds", "sequence": "contig_1", "start": 574337, "stop": 574540, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGGTAAGAAATAATAATCTTTCTTTTCAACCTTCAAGCAACAATCTCCAATTAGTTCAAAATCAAAACAGTCAAGCAAGTAATGGAATAGCTGATCCTAACTTTTCAAATGTTCAAACTGCATACAATAAAATTCACCAGCCCAATAACACTCCTGAAAAAACCCTTGATGCAGTTTATGCAGCAGTACTTGATCAATGA", "aa": "MMVRNNNLSFQPSSNNLQLVQNQNSQASNGIADPNFSNVQTAYNKIHQPNNTPEKTLDAVYAAVLDQ", "aa_hexdigest": "d66d5819d222054a4e5f497609e4c792", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7419.027700000002, "isoelectric_point": 5.4106969833374015 }, "id": "JPCNHPFPBM_1040", "locus": "GCA000292505_01040" }, { "type": "cds", "sequence": "contig_1", "start": 574563, "stop": 574823, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCTTTATCACTATCACTAAAACCTGGTAAGGAATTGAGAATTGTATCTTTCTCAGATTCAACTTTCTTTTTAAGTCAATCTTTATTAATTTTTAGTTCAAATTTCTTAAGTTGCTTAAGTTTATTTTCAAGATCTCAATCTCCAAAAGAGGAATTATTGGCTATAGTTTTAGCATTAGTTTCTAACCACTTTAGCTGTTCATTATCTAGCTTATTTAATTTAGAAATATCTAAATTAAAAGTTAATGTAAAACTTTGA", "aa": "MSLSLSLKPGKELRIVSFSDSTFFLSQSLLIFSSNFLSCLSLFSRSQSPKEELLAIVLALVSNHFSCSLSSLFNLEISKLKVNVKL", "aa_hexdigest": "335e368951636c6eb6485f6b20769a03", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9517.088699999991, "isoelectric_point": 9.184117698669432 }, "id": "JPCNHPFPBM_1041", "locus": "GCA000292505_01041" }, { "type": "cds", "sequence": "contig_1", "start": 574952, "stop": 575827, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTCTTTTTTTAAACAGATATTTAAGTCGCTTAAAAAGTTCTTCTTTTTACTATTTGGGATTATCTTTGTTTTATTTAGCATTATTTTCCTTGAAACATCTATTGTTCAGTTAAGTAATAACTTAGTTAGTACCTACACTACATTAGTATCTAAAACTAACTCTTCTGATATAGTAGCTCCTGCTATTTTAAAGGAAGCTAATCCTGTTTATACAGCAAGTTTAACTAATGATAGTGGTTATTTTTCCAAAATAAAGATAGATGATAAAAAAATTAACTATCTTTTTCCTTATCAAGAAAATGATTTTGGAAGTGATTCAGGACAAAGTAATGGTAGTGAGGATAACCAAAACAAAACCATTCCCCGCAAAGGCGATGTTAATGAAAAAGATAAACTCTTTTTAGCAAGAAAAAGAGGTATTTTAAAAGCATATGGAGAAGCCAATATTGCTGAAAAAAGAATTTATAAAGGGTTAGCAGTTTCTTTTAATAATACTGATTCTTTTAATGGTAATGATATTAGTGATTCCATTACAAATAGACACATAATATCAGACCCTCAAAACCTTATTTATGATGCTAGTGGTAACCTTCTAGGTTATTTTGCTTATGGTTTAATTAAAGAAACTATCTCACTGCGTGCTGGCATTGCGCGTTTTCCTGGTGATAAAGGAAAAAGCACAGGAACACAAATAAAAATTACACAAAAGCAACAAACAAACACTGATCCACAAAAAGACAATACTGTTAATTCACTTTATAAGACCAATAACAAAGATAAAGTTTGGTTTAAGAGTGATGAGACCAAAGCAGATAATACTGATATATCTGCTAACTATCTATTTACAGGTGGTAATGAAGCTGCTAACTGA", "aa": "MFSFFKQIFKSLKKFFFLLFGIIFVLFSIIFLETSIVQLSNNLVSTYTTLVSKTNSSDIVAPAILKEANPVYTASLTNDSGYFSKIKIDDKKINYLFPYQENDFGSDSGQSNGSEDNQNKTIPRKGDVNEKDKLFLARKRGILKAYGEANIAEKRIYKGLAVSFNNTDSFNGNDISDSITNRHIISDPQNLIYDASGNLLGYFAYGLIKETISLRAGIARFPGDKGKSTGTQIKITQKQQTNTDPQKDNTVNSLYKTNNKDKVWFKSDETKADNTDISANYLFTGGNEAAN", "aa_hexdigest": "fbb3b66d59cb64c3c0b747cb279ea35c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 32513.103900000016, "isoelectric_point": 9.034228706359862 }, "id": "JPCNHPFPBM_1042", "locus": "GCA000292505_01042" }, { "type": "cds", "sequence": "contig_1", "start": 575839, "stop": 576687, "strand": "-", "frame": 1, "gene": "lolD", "product": "Putative ABC transporter ATP-binding protein MG468-1", "db_xrefs": [ "COG:COG1136", "COG:M", "GO:0005524", "GO:0016887", "SO:0001217", "UniRef:UniRef50_Q50316", "UniRef:UniRef90_Q9ZB70" ], "nt": "ATGGTTTTAAAAACAAAAGAGAACAAAAAGTTTGATATTTACTTAAAATCATCAGATTTTGCTGTTAGTAAAAAAGCATCTAAATTAATTAAAAAGTTAAATAAAAAGCACCCCAAAAGAAAATCACTTAATTCATTTGAAGCTAAAAAGTATGATATTTACTTCAAAGAAGTTTGTAAAGCTGTTACAAATGGCATTAACAACCAACTTATTTGTAACCACATTAATTTAAAAATTCTTCCAGGAGAATTTGTTGTTATTCTTGGTAAATCTGGTAGTGGTAAAACCAGCTTACTTTCTTTAATTTCCGCATTAGATCGACCAACTAGCGGCGATAGTTTTGTTTGTGGAACTAACACCATATGTTGTAGTGATGCAAAACTAACCGCGTTAAGAAATAAAAACGTTGGTTATATTTTTCAACAATATGGTTTATTGCGTGATCTTGACGTTGATGATAATATTAAGCTTGCTCTACCATTAAAAAAAAGGTTTAATAATAATCTTGAAGAATTATTAGAACGCTTGGAATTAAAAGAACATAGACATAAGAAAGTTCATAAACTCTCTGGTGGACAACAACAACGTGTTGCTATTGCTCGGGCTTTAATTAAAGAACCTAAAATTTTGTTTGGTGATGAACCTACTGGAGCAGTTAATATTGATATTTCCAAAAAAATATTGCAATTTTTTGTGGAATATAATCGTGACAAAGGTACTACTATAGTAATAGTTACCCACAACGAAAAGATAGTGGAATTAGCTAAACGGGTAATTAAAATCCATGATGGCAAGATAATCGTTGATTATTTAAATCAGAATCCAAAAACCATCGAACAAATTAACTGA", "aa": "MVLKTKENKKFDIYLKSSDFAVSKKASKLIKKLNKKHPKRKSLNSFEAKKYDIYFKEVCKAVTNGINNQLICNHINLKILPGEFVVILGKSGSGKTSLLSLISALDRPTSGDSFVCGTNTICCSDAKLTALRNKNVGYIFQQYGLLRDLDVDDNIKLALPLKKRFNNNLEELLERLELKEHRHKKVHKLSGGQQQRVAIARALIKEPKILFGDEPTGAVNIDISKKILQFFVEYNRDKGTTIVIVTHNEKIVELAKRVIKIHDGKIIVDYLNQNPKTIEQIN", "aa_hexdigest": "ef08384f9e865f7a80c59c3d5d757ed0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9ZB70", "query_cov": 1.0, "subject_cov": 0.9929577464788732, "identity": 1.0, "score": 542.0, "evalue": 1.64e-194, "valid": true, "gene": "LolD", "product": "Putative ABC transporter ATP-binding protein MG468.1", "uniref50_id": "UniRef50_Q50316", "cog_id": "COG1136", "cog_category": "M", "go_ids": [ "GO:0005524", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_Q50316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q50316" ], "product": "Putative ABC transporter ATP-binding protein MG468.1 homolog" }, "genes": [ "lolD" ], "id": "JPCNHPFPBM_1043", "locus": "GCA000292505_01043" }, { "type": "cds", "sequence": "contig_1", "start": 576750, "stop": 578063, "strand": "-", "frame": 2, "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "db_xrefs": [ "COG:COG0593", "COG:L", "GO:0003688", "GO:0005524", "GO:0005737", "GO:0005886", "GO:0006260", "GO:0006270", "GO:0006275", "GO:0016887", "KEGG:K02313", "RefSeq:WP_014894707.1", "SO:0001217", "UniParc:UPI00027B41B3", "UniRef:UniRef100_UPI00027B41B3", "UniRef:UniRef50_P35888", "UniRef:UniRef90_P35888" ], "nt": "ATGGAACAATTTAATGCCTTTAAATCTCTATTAAAAAAGCATTATGAAAAAACAATAGGTTTTCATGATAAATACATTAAAGACATTAATCGTTTCGTATTTAAAAATAATGTTCTTTTAATTCTTTTAGAAAATGAATTTGCTCGTAATTCCTTAAATGATAATTCTGAAATTATTCATTTAGCTGAAAGTTTGTATGAAGGAATTAAAAGTGTTAATTTTGTTAATGAGCAAGATTTCTTTTTTAACTTAGCAAAATTAGAAGAAAATAGTCGTGATACTCTTTATCAAAATTCTGGATTGAGTAAAAACTATACTTTTCAAAACTTTGTAATTAGTGAAGGAAATAAAAGAGCTTATGAAGCAGGCGTTAGATTAGCTGAAACTCAAGATAACGAATTTTCACCGCTTTTTATTTACGGAGAAACCGGTCTTGGTAAAACTCACCTACTACAAGCAATAGGAAATGAAAAATTTCGTAATTTTCCAAATGCCAGAGTAAAGTATGTTGTTTCAAGTGATTTTGCCCAAGAAGTTGTTGATGCTTTTTATCAAAGGGATAAAGGTATAGAAAAACTAAAAAAAAATTATGAAAATTTAGATTTAGTTTTAATAGATGACACTCAAATATTTGGCAGAAAAGAAAAAACCTTAGAAATTCTTTTCAATATTTTTAATAACCTAGTTCTAAATAAAAAACAAATTGTTTTAGTTTCTGATAAGGCTCCTGATGAACTAATTGATATTGATGCAAGAATGATTTCTCGCTTTAAATCAGGATTATTACTAAAGATAGAAAAGCATAATTTGTCTTCACTTTGTGAAATACTTACTGTTAAATTAAAAGAAAAAGATCCTAACATCCAAATAACTAATGAGGCAAGACATGATGCAGCACAAATTTCAGGTAACGATGTGCGTGCTTTAAATGGAATTGCAACAAAGTTATTATTTTTTGCTAAAACTTCAAAACAAAATTTAATAAATACTGAAAATTTAAAAGAAATTCTTTTTGAAGAATTTGAGAAGTTTCATAAAAAGAGCTTTGATCCTTATTTATTAATAGAGAACGTTTGCCGTAGATTTAATGTTCCTATGGACAGTGTACTTTCAGAAAATCGTAAAGCAGAACTTGTCCGTGTTCGTGATGTGTGTAATTACCTTTTGCGTCAAAAGTACAACATGCAATTTCAACAAATTGGCAAAATATTTAAGAGAAGTCATTCAAGTGTATTAATGGCAGTTAAAAGAGTTGCTAAAATGATTGAAAATGACAGTTCATTACGGAATGTAATTACATCATTAGTAATTTAG", "aa": "MEQFNAFKSLLKKHYEKTIGFHDKYIKDINRFVFKNNVLLILLENEFARNSLNDNSEIIHLAESLYEGIKSVNFVNEQDFFFNLAKLEENSRDTLYQNSGLSKNYTFQNFVISEGNKRAYEAGVRLAETQDNEFSPLFIYGETGLGKTHLLQAIGNEKFRNFPNARVKYVVSSDFAQEVVDAFYQRDKGIEKLKKNYENLDLVLIDDTQIFGRKEKTLEILFNIFNNLVLNKKQIVLVSDKAPDELIDIDARMISRFKSGLLLKIEKHNLSSLCEILTVKLKEKDPNIQITNEARHDAAQISGNDVRALNGIATKLLFFAKTSKQNLINTENLKEILFEEFEKFHKKSFDPYLLIENVCRRFNVPMDSVLSENRKAELVRVRDVCNYLLRQKYNMQFQQIGKIFKRSHSSVLMAVKRVAKMIENDSSLRNVITSLVI", "aa_hexdigest": "2ad5eeff0f673144650257057fd8583d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00027B41B3", "ncbi_nrp_id": "WP_014894707.1", "uniref100_id": "UniRef100_UPI00027B41B3", "db_xrefs": [ "SO:0001217", "UniParc:UPI00027B41B3", "RefSeq:WP_014894707.1", "UniRef:UniRef100_UPI00027B41B3" ] }, "ips": { "uniref100_id": "UniRef100_UPI00027B41B3", "uniref90_id": "UniRef90_P35888" }, "psc": { "uniref90_id": "UniRef90_P35888", "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "uniref50_id": "UniRef50_P35888", "cog_id": "COG0593", "cog_category": "L", "kegg_orthology_id": "K02313", "go_ids": [ "GO:0005737", "GO:0005886", "GO:0005524", "GO:0016887", "GO:0003688", "GO:0006260", "GO:0006270", "GO:0006275" ] }, "pscc": { "uniref50_id": "UniRef50_P35888", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P35888" ], "product": "Chromosomal replication initiator protein DnaA" }, "genes": [ "dnaA" ], "id": "JPCNHPFPBM_1044", "locus": "GCA000292505_01044" }, { "type": "oriC", "sequence": "contig_1", "start": 578064, "stop": 578705, "strand": "?", "product": "origin of replication", "nt": "TATTCTTCTATAACATTGTCAAGAATGATAGTTAAAATTCTCGAAATTGGGATATTAACTGCTTTGGAGTAATTTCTAACTTTTTGTCATACTCTTTGACTTGTATAGAAGTGTACACCTGTATCTAGTTTTTCTTGGCGTTCAACAGGAACTATTCCTGGTATTTTTGTTTTAGGTTGGGGAGGAATAGGCTGTGGTTGTGTGAATTGTTGTTGAAAATTTTGATTTTTTTGCTGTAAGAAACCATTATTATGATATTGAAAATTTTGTTCCTCTTTAAAATATCTCTCTTTTTTTGGTTTTCCAGAAAAATTTGATGAAAAAGATTTTTCTTCATTTCAATTTTCAAGATTATTTTCATTTTGTTGATTTATTTGCTCAGGCTGTTGAAATGAATTATTTTTTGATCAAAAAGATTTTGGAAAGGTTTTTTCAAAAGCAGATAAAGGTCCAAAATCAAATGAAGATGAATCTTTGTCAAAAGATGTTTCTTCTCTTTTTGACAAATTTTGTTTTTGATTAAACTTATTTTTATTTTGGGGTGTTACTTTTTCTTTTATGGAAAACAAATCTTCTTCTAAAAGACTTTGTTCTGGGTCATCATCTTGTGCTAAATCAAAGAAAAAACGTTTCTTTTTGTTA", "id": "JPCNHPFPBM_1047" }, { "type": "cds", "sequence": "contig_1", "start": 578471, "stop": 578716, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCCATTAATAACAAAAAGAAACGTTTTTTCTTTGATTTAGCACAAGATGATGACCCAGAACAAAGTCTTTTAGAAGAAGATTTGTTTTCCATAAAAGAAAAAGTAACACCCCAAAATAAAAATAAGTTTAATCAAAAACAAAATTTGTCAAAAAGAGAAGAAACATCTTTTGACAAAGATTCATCTTCATTTGATTTTGGACCTTTATCTGCTTTTGAAAAAACCTTTCCAAAATCTTTTTGA", "aa": "MSINNKKKRFFFDLAQDDDPEQSLLEEDLFSIKEKVTPQNKNKFNQKQNLSKREETSFDKDSSSFDFGPLSAFEKTFPKSF", "aa_hexdigest": "a134ba9314829478f9d26a2092170a99", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9534.465299999993, "isoelectric_point": 5.4164945602416985 }, "id": "JPCNHPFPBM_1045", "locus": "GCA000292505_01045" }, { "type": "cds", "sequence": "contig_1", "start": 578706, "stop": 579515, "strand": "-", "frame": 2, "gene": "parA", "product": "ParA family protein MG470", "db_xrefs": [ "COG:COG1192", "COG:DN", "KEGG:K03496", "RefSeq:WP_009885563.1", "SO:0001217", "UniParc:UPI000013131F", "UniRef:UniRef100_P47706", "UniRef:UniRef50_P47706", "UniRef:UniRef90_P47706" ], "nt": "ATGATCATTTCTTTTGTAAATAATAAAGGCGGCGTATTAAAAACTACAATGGCAACTAATGTTGCAGGATCATTGGTTAAACTTTGTCCAGAAAGGCGAAAAGTCATTCTTGATCTTGACGGGCAGGGGAATGTTTCTGCATCATTTGGACAAAACCCTGAAAGATTGAATAACACGTTAATTGACATTTTGTTGAAGGTTCCAAAATTCAGTGGTTCAAATAATTTCATTGAAATAGATGATTGCTTGCTATCTGTATATGAGGGACTTGATATTCTTCCTTGTAATTTTGAACTTAATTTTGCTGATATAGACATATCACGTAAAAAATATAAGGCCTCAGATATAGCAGAAATAGTTAAGCAACTAGCAAAAAGATATGAATTTGTTTTATTAGATACACCACCAAATATGGCAACTTTAGTTTCTACTGCAATGAGTTTATCAGATGTCATAGTTATTCCCTTTGAACCAGATCAATATTCGATGCTAGGTTTGATGAGAATAGTTGAAACAATAGATACATTTAAGGAAAAAAATACAAATCTTAAAACTATTTTAGTTCCTACAAAAGTTAACGTTCGTACTCGTCTTCATAATGAAGTAATAGATTTAGCTAAAACAAAAGCTAAAAAAAATAATGTAGCTTTTTCTAAAAATTTTGTTTCTCTGACATCAAAATCATCAGCAGCTGTAGGTTATGAAAAATTGCCAATATCATTAGTATCATCACCAAGTAAAAAATATCTAAATGAGTATTTAGAAATAACAAAAGAAATCCTAAATTTAGCAAATTACAATGTCCATTAA", "aa": "MIISFVNNKGGVLKTTMATNVAGSLVKLCPERRKVILDLDGQGNVSASFGQNPERLNNTLIDILLKVPKFSGSNNFIEIDDCLLSVYEGLDILPCNFELNFADIDISRKKYKASDIAEIVKQLAKRYEFVLLDTPPNMATLVSTAMSLSDVIVIPFEPDQYSMLGLMRIVETIDTFKEKNTNLKTILVPTKVNVRTRLHNEVIDLAKTKAKKNNVAFSKNFVSLTSKSSAAVGYEKLPISLVSSPSKKYLNEYLEITKEILNLANYNVH", "aa_hexdigest": "e8f293204ff28c39358c46de4a80ed83", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000013131F", "ncbi_nrp_id": "WP_009885563.1", "uniref100_id": "UniRef100_P47706", "db_xrefs": [ "SO:0001217", "UniParc:UPI000013131F", "RefSeq:WP_009885563.1", "UniRef:UniRef100_P47706" ] }, "ips": { "uniref100_id": "UniRef100_P47706", "uniref90_id": "UniRef90_P47706" }, "psc": { "uniref90_id": "UniRef90_P47706", "gene": "ParA", "product": "ParA family protein MG470", "uniref50_id": "UniRef50_P47706", "cog_id": "COG1192", "cog_category": "DN", "kegg_orthology_id": "K03496" }, "pscc": { "uniref50_id": "UniRef50_P47706", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47706" ], "product": "ParA family protein MG470" }, "genes": [ "parA" ], "id": "JPCNHPFPBM_1046", "locus": "GCA000292505_01046" } ], "sequences": [ { "id": "contig_1", "description": "[organism=Mycoplasmoides genitalium] [strain=M2288] [gcode=11] [completeness=complete] [topology=circular]", "nt": "TAAGTTATTATTTAGTTAATACTTTTAACAATATTATTAAGGTATTTAAAAAATACTATTATAGTATTTAACATAGTTAAATACCTTCCTTAATACTGTTAAATTATATTCAATCAATACATATATAATATTATTAAAATACTTGATAAGTATTATTTAGATATTAGACAAATACTAATTTTATATTGCTTTAATACTTAATAAATACTACTTATGTATTAAGTAAATATTACTGTAATACTAATAACAATATTATTACAATATGCTAGAATAATATTGCTAGTATCAATAATTACTAATATAGTATTAGGAAAATACCATAATAATATTTCTACATAATACTAAGTTAATACTATGTGTAGAATAATAAATAATCAGATTAAAAAAATTTTATTTATCTGAAACATATTTAATCAATTGAACTGATTATTTTCAGCAGTAATAATTACATATGTACATAGTACATATGTAAAATATCATTAATTTCTGTTATATATAATAGTATCTATTTTAGAGAGTATTAATTATTACTATAATTAAGCATTTATGCTTAATTATAAGCTTTTTATGAACAAAATTATAGACATTTTAGTTCTTATAATAAATAATAGATATTAAAGAAAATAAAAAAATAGAAATAAATATCATAACCCTTGATAACCCAGAAATTAATACTTAATCAAAAATGAAAATATTAATTAATAAAAGTGAATTGAATAAAATTTTGAAAAAAATGAATAACGTTATTATTTCCAATAACAAAATAAAACCACATCATTCATATTTTTTAATAGAGGCAAAAGAAAAAGAAATAAACTTTTATGCTAACAATGAATACTTTTCTGTCAAATGTAATTTAAATAAAAATATTGATATTCTTGAACAAGGCTCCTTAATTGTTAAAGGAAAAATTTTTAACGATCTTATTAATGGCATAAAAGAAGAGATTATTACTATTCAAGAAAAAGATCAAACACTTTTGGTTAAAACAAAAAAAACAAGTATTAATTTAAACACAATTAATGTGAATGAATTTCCAAGAATAAGGTTTAATGAAAAAAACGATTTAAGTGAATTTAATCAATTCAAAATAAATTATTCACTTTTAGTAAAAGGCATTAAAAAAATTTTTCACTCAGTTTCAAATAATCGTGAAATATCTTCTAAATTTAATGGAGTAAATTTCAATGGATCCAATGGAAAAGAAATATTTTTAGAAGCTTCTGACACTTATAAACTATCTGTTTTTGAGATAAAGCAAGAAACAGAACCATTTGATTTCATTTTGGAGAGTAATTTACTTAGTTTCATTAATTCTTTTAATCCTGAAGAAGATAAATCTATTGTTTTTTATTACAGAAAAGATAATAAAGATAGCTTTAGTACAGAAATGTTGATTTCAATGGATAACTTTATGATTAGTTACACATCGGTTAATGAAAAATTTCCAGAGGTAAACTACTTTTTTGAATTTGAACCTGAAACTAAAATAGTTGTTCAAAAAAATGAATTAAAAGATGCACTTCAAAGAATTCAAACTTTGGCTCAAAATGAAAGAACTTTTTTATGCGATATGCAAATTAACAGTTCTGAATTAAAAATAAGAGCTACTGTTAATAATATCGGAAATTCTCTTGAGGAAATTTCTTGTCTTAAATTTGAAGGTTATAAACTTAATATTTCTTTTAACCCAAGTTCTCTATTAGATCACATAGAGTCTTTTGAATCAAATGAAATAAATTTTGATTTCCAAGGAAATAGTAAGTATTTTTTGATAACCTCTAAAAGTGAACCTGAACTTAAGCAAATATTGGTTCCTTCAAGATAATGAATCTTTACGATCTTTTAGAACTACCAACTACAGCATCAATAAAAGAAATAAAAATTGCTTATAAAAGATTAGCAAAGCGTTATCACCCTGATGTAAATAAATTAGGTTCGCAAACTTTTGTTGAAATTAATAATGCTTATTCAATATTAAGTGATCCTAACCAAAAGGAAAAATATGATTCAATGCTGAAAGTTAATGATTTTCAAAATCGCATCAAAAATTTAGATATTAGTGTTAGATGACATGAAAATTTCATGGAAGAACTCGAACTTCGTAAGAACTGAGAATTTGATTTTTTTTCATCTGATGAAGATTTCTTTTATTCTCCATTTACAAAAAACAAATATGCTTCCTTTTTAGATAAAGATGTTTCTTTAGCTTTTTTTCAGCTTTACAGCAAGGGCAAAATAGATCATCAATTGGAAAAATCTTTATTGAAAAGAAGAGATGTAAAAGAAGCTTGTCAACAGAATAAAAATTTTATTGAAGTTATAAAAGAGCAATATAACTATTTTGGTTGAATTGAAGCTAAGCGTTATTTCAATATTAATGTTGAACTTGAGCTCACACAGAGAGAGATAAGAGATAGAGATGTTGTTAACCTACCTTTAAAAATTAAAGTTATTAATAATGATTTTCCAAATCAACTCTGATATGAAATTTATAAAAACTATTCATTTCGCTTATCTTGAGATATAAAAAATGGTGAAATTGCTGAATTTTTCAATAAAGGTAATAGAGCTTTAGGATGAAAAGGTGACTTAATTGTCAGAATGAAAGTAGTTAATAAAGTAAACAAAAGACTGCGTATTTTTTCAAGCTTTTTTGAGAACGATAAATCTAAATTATGGTTCCTTGTTCCAAACGATAAACAAAGTAATCCTAATAAGGGCGTTTTTAACTATAAAACTCAGCACTTTATTGATTAAAAAACCTTTCATTTTTAATGTGTTATAATTATTTGTTATGCCATAAATTTAGTTTGTGGCAAAAGCTTCTGTACTGTTTATTTAATGGAAGAAAATAACAAAGCAAATATCTATGACTCTAGTAGCATTAAGGTCCTTGAAGGACTTGAGGCTGTTAGAAAACGCCCTGGAATGTACATTGGTTCTACTGGCGAAGAAGGTTTGCATCACATGATCTGAGAGATAGTAGACAACTCAATTGATGAAGCAATGGGAGGTTTTGCCAGTTTTGTTAAGCTTACCCTTGAAGATAATTTTGTTACCCGTGTAGAGGATGATGGAAGAGGGATACCTGTTGATATCCATCCTAAGACTAATCGTTCTACAGTTGAAACAGTTTTTACAGTTCTACACGCTGGCGGTAAATTTGATAACGATAGCTATAAAGTGTCAGGTGGTTTACACGGTGTTGGTGCATCAGTTGTTAATGCGCTTAGTTCTTCTTTTAAAGTTTGAGTTTTTCGTCAAAATAAAAAGTATTTTCTCAGCTTTAGCGATGGAGGAAAGGTAATTGGAGATTTGGTCCAAGAAGGTAACTCTGAAAAAGAGCATGGAACAATTGTTGAGTTTGTTCCTGATTTCTCTGTAATGGAAAAGAGTGATTACAAACAAACTGTAATTGTAAGCAGACTCCAGCAATTAGCTTTTTTAAACAAGGGAATAAGAATTGACTTTGTTGATAATCGTAAACAAAACCCACAGTCTTTTTCTTGAAAATATGATGGGGGATTGGTTGAATATATCCACCACCTAAACAACGAAAAAGAACCACTTTTTAATGAAGTTATTGCTGATGAAAAAACTGAAACTGTAAAAGCTGTTAATCGTGATGAAAACTACACAGTAAAGGTTGAAGTTGCTTTTCAATATAACAAAACATACAACCAATCAATTTTCAGTTTTTGTAACAACATTAATACTACAGAAGGTGGAACCCATGTGGAAGGTTTTCGTAATGCACTTGTTAAGATCATTAATCGCTTTGCTGTTGAAAATAAATTCCTAAAAGATAGTGATGAAAAGATTAACCGTGATGATGTTTGTGAAGGATTAACTGCTATTATTTCCATTAAACACCCAAACCCACAATATGAAGGACAAACTAAAAAGAAGTTAGGTAATACTGAGGTAAGACCTTTAGTTAATAGTGTTGTTAGTGAAATCTTTGAACGCTTCATGTTAGAAAACCCACAAGAAGCAAACGCTATCATCAGAAAAACACTTTTAGCTCAAGAAGCGAGAAGAAGAAGTCAAGAGGCTAGGGAGTTAACTCGTCGTAAATCACCTTTTGATAGTGGTTCATTACCAGGTAAATTAGCTGATTGTACAACCAGAGATCCTTCGATTAGTGAACTTTACATTGTTGAGGGTGATAGTGCTGGTGGCACTGCTAAAACAGGAAGAGATCGTTATTTTCAAGCTATCTTACCCTTAAGAGGAAAGATTTTAAACGTTGAAAAATCTAACTTTGAACAAATCTTTAATAATGCAGAAATTTCTGCATTAGTGATGGCAATAGGCTGTGGGATTAAACCTGATTTTGAACTTGAAAAACTTAGATATAGCAAGATTGTGATCATGACAGATGCTGATGTTGATGGTGCACACATAAGAACACTTCTCTTAACTTTCTTTTTTCGCTTTATGTATCCTTTGGTTGAACAAGGCAATATTTTTATTGCTCAACCCCCACTTTATAAAGTGTCATATTCCCATAAGGATTTATACATGCACACTGATGTTCAACTTGAACAGTGAAAAAGTCAAAACCCTAACGTAAAGTTTGGGTTACAAAGATATAAAGGACTTGGAGAAATGGATGCATTGCAGCTGTGAGAAACAACAATGGATCCTAAGGTTAGAACATTGTTAAAAGTTACTGTTGAAGATGCTTCTATTGCTGATAAAGCTTTTTCACTGTTGATGGGTGATGAAGTTCCCCCAAGAAGAGAATTTATTGAAAAAAATGCTCGTAGTGTTAAAAACATTGATATTTAATTTGGTTAGTATAAATGGCAAAGCAACAAGATCAAGTAGATAAGATTCGTGAAAACTTAGACAATTCAACTGTCAAAAGTATTTCATTAGCAAATGAACTTGAGCGTTCATTCATGGAATATGCTATGTCAGTTATTGTTGCTCGTGCTTTACCTGATGCTAGAGATGGACTTAAACCAGTTCATCGTCGTGTTCTTTATGGTGCTTATATTGGTGGCATGCACCATGATCGTCCTTTTAAAAAGTCTGCGAGGATTGTTGGTGATGTAATGAGTAAATTCCACCCTCATGGTGATATGGCAATATATGACACCATGTCAAGAATGGCTCAAGACTTTTCATTAAGATACCTTTTAATTGATGGTCATGGTAATTTTGGTTCTATAGATGGTGATAGACCTGCTGCACAACGTTATACAGAAGCAAGATTATCTAAACTTGCAGCAGAACTTTTAAAAGATATTGATAAAGATACAGTTGACTTTATTGCTAATTATGATGGTGAGGAAAAAGAACCAACTGTTCTACCAGCAGCTTTCCCTAACTTACTTGCAAATGGTTCTAGTGGGATTGCAGTTGGAATGTCAACATCTATTCCTTCCCATAATCTCTCTGAATTAATTGCGGGTTTAATCATGTTAATTGATAATCCTCAATGCACTTTTCAAGAATTATTAACTGTAATTAAAGGACCTGATTTTCCAACAGGAGCTAACATTATCTACACAAAAGGAATTGAAAGCTACTTTGAAACAGGTAAAGGCAATGTAGTAATTCGTTCTAAAGTTGAGATAGAACAATTGCAAACAAGAAGTGCATTAGTTGTAACTGAAATTCCTTACATGGTTAACAAAACTACCTTAATTGAAAAGATTGTAGAACTTGTTAAAGCTGAAGAGATTTCAGGAATTGCTGATATCCGTGATGAATCCTCTCGAGAAGGAATAAGGTTAGTGATTGAAGTAAAACGCGACACTGTACCTGAAGTTTTATTAAATCAACTTTTTAAATCAACAAGATTACAAGTACGCTTCCCTGTTAATATACTTGCTTTAGTTAAAGGAGCTCCTGTACTTCTCAACATGAAACAAGCTTTGGAAGTATATCTTGATCATCAAATTGATGTTCTTGTTAGAAAAACAAAGTTTGTGCTTAATAAACAACAAGAACGTTATCACATTTTAAGCGGACTTTTAATTGCTGCTTTAAATATTGATGAGGTTGTTGCAATTATTAAAAAATCAGCAAATAACCAGGAAGCAATTAATACATTAAATACAAAGTTTAAGCTTGATGAAATTCAAGCTAAAGCAGTTCTTGACATGCGTTTAAGGAGCTTAAGCGTACTTGAAGTTAACAAACTTCAAACTGAACAAAAAGAGTTAAAAGATTCAATTGAATTTTGTAAGAAAGTGTTAGCTGATCAAAAATTACAGCTAAAAATAATCAAAGAGGAATTGCAAAAAATCAATGATCAGTTTGGTGATGAAAGAAGAAGTGAAATTCTCTATGATATCTCTGAGGAAATTGATGATGAATCATTGATAAAAGTTGAGAATGTAGTGATAACTATGTCTACAAATGGTTATCTAAAAAGGATTGGAGTTGATGCTTATAATCTTCAACATCGTGGTGGAGTTGGGGTTAAAGGGCTAACTACTTATGTTGATGATAGTATTAGTCAATTATTGGTCTGTTCAACTCACTCTGACTTATTATTTTTTACTGATAAGGGTAAGGTTTATAGAATTAGAGCTCATCAAATTCCCTATGGTTTTAGAACAAATAAAGGTATTCCCGCTGTTAACTTAATCAAAATTGAAAAGGATGAAAGAATTTGTTCATTGTTATCTGTTAATAACTATGATGATGGTTATTTCTTTTTCTGTACTAAAAATGGAATTGTTAAAAGAACGAGCTTGAATGAATTCATCAACATCTTAAGTAATGGTAAGCGGGCTATATCTTTTGATGATAATGACACTTTGTATTCAGTAATTAAAACCCACGGAAATGATGAGATTTTTATTGGTTCTACCAATGGATTTGTTGTTCGATTCCATGAAAATCAACTCAGAGTTCTTTCAAGAACAGCAAGAGGTGTATTTGGTATCAGTTTAAATAAAGGAGAATTTGTTAATGGACTATCAACTTCAAGCAACGGTAGCTTACTTTTATCAGTCGGTCAAAATGGAATAGGTAAATTAACGAGCATAGATAAATATAGGCTCACAAAACGTAATGCTAAGGGAGTTAAAACTCTAAGGGTTACTGATAGAACAGGCCCTGTTGTTACAACAACCACTGTTTTTGGTAATGAGGATCTTTTAATGATTTCCTCTGCTGGTAAAATTGTGCGTACCAGTTTACAAGAACTTTCAGAACAAGGTAAAAACACTTCTGGTGTTAAGTTAATTAGATTAAAAGATAATGAACGTTTAGAAAGAGTAACTATCTTTAAAGAAGAGTTAGAAGACAAAGAAATGCAACTAGAAGATGTTGGATCCAAACAAATTACGCAATAACTATGATTTCTTTAAAAAGAAACTGTTAGAAAGAAATGTAAATGAGCAATTATTAAATCAGTTTATTCAAACTGATAAACTAATGCGCAAAAACTTGCAACAACTTGAACTTGCTAACCAAAAACAAAGCTTGTTGGCAAAACAAGTTGCTAAGCAAAAAGATAATAAAAAGCTATTAGCTGAATCAAAAGAACTTAAGCAGAAGATTGAAAACTTAAATAGTGCTTATAAAGATTCACAAAACATTAGTCAGGATTTACTTCTAAATTTTCCTAATATTGCTCATGAATCAGTTCCTGTTGGTAAAAATGAATCAGCAAACTTAGAACTCCTTAAAGAAGGGAGAAAACCAGTTTTTGATTTCAAACCTTTACCACATTGGGAGTTATGTGAAAAGTTAAATTTAGTTGCTTTTGATAAAGCTACTAAGATTAGTGGAACTAGGTTTGTTGCATATACAGATAAAGCAGCTAAACTGCTTAGAGCGATAACTAATCTAATGATTGACCTTAATAAAAGCAAGTATCAAGAATGAAACCTGCCAGTTGTTATTAATGAATTAAGTTTAAGATCAACCGGACAACTACCTAAGTTTAAAGATGATGTTTTTAAACTAGAAAACACCCGTTATTATCTTTCTCCAACTTTAGAGGTACAACTTATCAATTTACATGCTAATGAAATTTTTAATGAAGAAGATTTACCTAAATACTACACTGCAACAGGTATTAACTTTCGTCAAGAAGCGGGTAGTGCTGGTAAACAAACCAAAGGAACTATTAGATTGCATCAGTTTCAAAAAACTGAGTTAGTTAAGTTTTGTAAACCTGAAAATGCTATCAATGAATTGGAAGCAATGGTTAGAGATGCTGAACAAATCTTAAAGGCACTTAAGTTACCTTTTAGAAGGTTATTGTTATGTACTGGTGATATGGGCTTTAGTGCTGAAAAAACATATGATCTTGAAGTTTGAATGGCAGCTAGCAATGAATATCGTGAAGTTTCTTCTTGTTCATCTTGTGGTGATTTTCAAGCAAGAAGAGCTATGATTCGTTACAAAGATATTAACAACGGTAAAAACAGTTATGTTGCTACTTTAAATGGAACAGCATTATCTATTGATAGAATTTTTGCTGCAATTCTAGAAAATTTTCAAACAAAAGATGGCAAAATTCTTATCCCACAAGCATTAAAAAAATACCTTGATTTTGACACAATCAAGTAATTTATGAATAAAGGTGTTTTTGTTGTTATTGAAGGAGTTGATGGAGCGGGCAAAACTGCTTTAATTGAAGGTTTTAAAAAACTTTATCCAACTAAGTTTTTGAACTATCAACTTACTTATACTAGAGAACCTGGTGGTACTTTGTTAGCTGAAAAAATTCGTCAACTTCTTTTAAATGAAACAATGGAACCTCTAACTGAAGCTTATTTGTTTGCCGCAGCTAGAACTGAACATATCAGTAAGCTAATTAAACCAGCAATTGAAAAAGAACAACTAGTTATTTCAGATAGATTTGTTTTCTCTAGTTTTGCATACCAAGGATTAAGCAAAAAAATAGGCATTGATACAGTAAAACAGATTAATCATCATGCGTTAAGAAATATGATGCCAAACTTTACCTTTATTTTGGATTGCAATTTTAAAGAAGTATTACAAAGGATGCAAAAGCGTGGTAATGATAATCTTCTTGATGAATTTATTAAAGGAAAGAATGATTTTGATACAGTTCGTTCTTATTATTTAAGCTTAGTTGATAAAAAAAACTGTTTCTTGATTAATGGTGATAATAAACAAGAACACCTAGAGAAATTTATTGAATTGTTAACAAGATGCTTACAACAACCCACGCATTACTAATCATTCAAAGAAAAGGTAGTTTCTTAAAACCTTTTCTTGATAATTATCTTACTAGTATTGTTTGTGAAAACAAAAATGGTTGCAAAAAGTGTATAAACTGTTTGGAAATTCTCAATAATAAATACAACAGCTTATATTGATTTGATCAAATTAATCCTTTCAAAAGAGAAAATGCCCTTCAGTTAGCAAGAATTTTTAACCGTGAAAGAACAAGTGTAAACAATAAAAATATTTATCTAATTGAAGAAATTGAAAAATTAAGCAGTAATTCTATAAATAGTTTATTGAGACTAGTTGAAGATAGTCCGATAAATAGTTATGGTATTTTTACAACTAAAAATGAAAGTTTAATTCTTTCCACTTTTTTAAGTAGAGTACAGAAAGTAGTTTTAAAAAAAGCTAGTAAAGTTCCTTTTAAAGTAAGCAAAAATGATCAAGAAATTATTACAAGTTTTTTTACTGTAGATGAACAAATTGAAGCAATTGAAAATGGAAGTTTTAACCGTTTCAAAATTATCTTAGATGCATGTTTAAACAAAAAAACAGGTACAGAACAAATTTATCATGCTTGACAAATTTTTAGAGATTTTTCTAATAGTGAAATTGCTCAGTTAATTACTCTAATAATTAATAAAACTGAAAATATAGATAAAAAATCAATTTTGTTTAATTGTTTAAAAGTTTTGCCATATAATCCTCCAAAATCCACTTTGTTTGCTAATTTAGTTAGTTGATAGTTATGAAAAGCGAAATTAATATTTTTGCACTAGCAACTGCACCTTTTAATAGTGCATTACATATTATTAGGTTTTCTGGTCCTGATGTTTATGAGATTTTAAACAAGATAACTAATAAAAAAATAACAAGAAAAGGGATGCAAATTCAACGCACATGGATAGTTGATGAAAACAATAAGCGAATTGATGATGTGCTATTATTTAAATTTGTCTCTCCAAATTCTTATACAGGAGAAGATTTAATTGAAATTTCTTGTCATGGTAACATGTTGATCGTTAATGAAATTTGCGCACTTCTTTTAAAAAAAGGAGGTGTTTATGCCAAACCTGGTGAATTTACCCAAAGGAGTTTTTTAAATGGAAAAATGAGTTTACAACAAGCTAGTGCTGTAAATAAATTGATTTTATCTCCTAACTTATTAGTTAAAGATATAGTCTTAAATAATTTAGCGGGTGAAATGGATCAACAATTAGAACAAATAGCTCAACAAGTTAATCAATTAGTAATGCAAATGGAAGTAAACATTGATTATCCAGAATATCTTGATGAACAAGTAGAACTATCAACTTTAAATAATAAAGTTAAATTGATTATTGAAAAGCTTAAAAGAATTATTGAAAATAGTAAACAACTCAAAAAACTTCACGATCCTTTTAAAATTGCCATTATAGGCGAAACTAATGTAGGTAAATCTTCTTTACTCAACGCTTTATTAAATCAAGATAAAGCGATAGTTTCAAATATTAAAGGTAGTACACGCGATGTTGTTGAAGGGGATTTCAATTTAAATGGTTATTTAATCAAGATCTTAGATACTGCAGGTATCCGTAAACATAAAAGTGGGCTTGAAAAAGCAGGAATTAAAAAAAGCTTTGAATCTATAAAGCAAGCTAATTTGGTTATTTATCTTTTAGATGCAACACATCCAAAGAAAGATCCTGAATTAATTAGTTTTTTTAAGAAAAATAAAAAGGATTTTTTTGTTTTCTATAACAAAAAAGATTTAATTACAAATAAGTTTGAAAATAGTATTTCTGCAAAGCAAAAAGATATTAAAGAATTAGTTGATTTATTAACTAAATATATTAACGAGTTTTATAAAAAAATAGATCAAAAAATCTATCTGATTGAAAATTGACAGCAAATTTTAATTGAAAAAATTAAAGAACAATTAGAACAGTTTTTAAAGCAACAAAAAAAATATTTATTTTTCGATGTTTTAGTTACCCATCTAAGAGAAGCTCAACAAGATATCCTTAAATTATTAGGTAAGGATGTAGGTTTTGATTTAGTTAATGAAATTTTTAATAATTTTTGTTTAGGAAAATAATGGAATACTTTGATGCACATTGTCATTTAAATTGCGAACCTTTACTGAGTGAAATTGAAAAAAGCATCGCTAATTTCAAATTAATTAATTTAAAAGCAAATGTTGTAGGTACAGATTTGGATAACTCTAAAATTGCTGTTGAATTAGCTAAAAAATATCCTGATCTTTTAAAAGCAACCATAGGTATCCATCCAAATGATGTTCATTTAGTTGATTTTAAAAAGACAAAAAAACAACTTAATGAACTATTAATAAATAACAGAAATTTCATAAGTTGTATTGGTGAATATGGTTTTGATTATCACTACACAACAGAATTTATTGAATTGCAAAACAAATTCTTTGAGATGCAATTTGAAATAGCTGAAACTAATAAATTGGTTCACATGCTTCATATTCGTGATGCTCATGAAAAAATTTATGAAATATTAACAAGATTAAAGCCAACTCAACCTGTGATTTTTCATTGTTTCAGTCAAGATATAAATATTGCTAAAAAGCTTCTATCATTAAAAGATTTAAATATTGACATCTTCTTTTCTATCCCAGGGATAGTTACTTTTAAGAATGCTCAAGCATTACACGAAGCTTTAAAGATTATTCCTAGTGAATTACTTTTAAGTGAAACTGATTCACCGTGATTAACCCCTTCTCCTTTTCGAGGCAAAGTTAACTGACCTGAATATGTAGTTCATACTGTTAGCACTGTTGCTGAAATAAAAAAAATAGAAATTGCTGAAATGAAGCGAATTATTGTTAAAAATGCAAAAAAATTATTTTGGCATTAAAAGTTAAATAAAGCAATTTATTTAACAAATGGATGTTAGAACTGAAAGACTAAACGAATTGTTTTTTGTTTATCATAAAAACTTAAAAAATCAATCTAAATCTAAATATAGCAGAGCAGTTAATTACTTAAAAAGGCGTGGATTTAATTTACAAGATTTTTTAAAAGTTGGGGGTGGTGTTGGTTATTTACAAAATAAAGAATGATTAAATTTACCTTTATACAGTTTTGATGGTAATTTAATTGGTTTTCTAAACAGAAAAGTTAGTTATAAAAAGGAATTTTTATATACACCTTTTAATAAACCTCCTTCAAAGAGCGAAGCTTTTGTAGGACTCAGAGAATTAGTTATTAAAGACAATAGCATATATCTTGTTGAAGGTGATTTTGACTGGTTAGCTTTTCGCAAAGCAGGTATATTAAATTCTCTTCCTCTTTGTGGTTTGAGTATTTCAAATCAACAAGTTCAATGATTAAAACAAAAAAAGATTAAAAAGATTTTTATCTGTTTTGATAATGATTTAGCTGGAAAGAATGGAGCAAAAAATTTAAAAGAATATCTAACTAAGCAAGGATTTATAACAAAAGTTATAGAAATTAAAGCTGCCGCAAAAGACTGAAATGATTTGTTTTTATTAAACAACTCAAATTGATCAGCGGTTTTAACTAATCAACTTCTTTTTTAAGAATTTTTTAAATTTACTAATAATTGTTCTGATAATTATTTTAGTGATATTTAAATCTGGACAAAGCTGAACTAAAGCTCTCGCACCAGCAGCATCTTCAATTTCATTAACAATAACCCTATTATATCTATTTAAAAAGAAGTCAATAGCATAATAACCTTCCCTTAGGCGTTTAGCTATTTTCTTTATTTTTCTTTTAGTAAATCACTTTAATTTAAACAAGGAAACTTCAGCACCTTGTGAAAAGTTAGCTTTAAATTGATTAGCATTAGAAATTTTTTTAATAACTTTAATTATTTTTCCAAACAAAATATAAGCACGATATTCAACTGTGCCAATTGATAAAAAAGGTTGAACAATTCATTCTGTTGCATTTTCAATGTTTAAATGTTTGATCTCGTCAGCACTATTAACTAAATGTACATCTTTTCCACCGTGTGAATTACGTTTCTTAACGATGGCAGGAAATGATTTGATTGTTTCTTTACTAAGAAGAGAAGAATTGACAGTTAGAAATCTATTTTGTTTTAATCATTTATATGTTTCGTATTTATCGTTTGCTATAAAAACAACATTAAAAGGATTAACTAAAAAAGTATTTATTTGATTATTGGTTTTTAAAAAATCTACTTTTCTTGAACGATTTAAAATCAATTCAGCTTCATTAATTTTAGCTTCGAAATTAATGTCTTTATTTTCAAGTAATAAGACTTCAGCATTTAGTTTTTTCTTTTGTAATTCCTTGATTAGACTTAAAGCAAATGTTTGATTTTTTTCAAAATCATCCTTGTTGTCATAAACAACTAATGCTTTTCTGTTTTTTAATTTAATTTTTCCCATTAATCTAAATTGCTTTTAAAAGCTCAATTGCAAGATTAGTATTTAAATACATTGAGCTTCTTGTTAATTGCACATTAGGATTTACTTCACAAAAGATCAATGATCTGTCTTGATCAAACAAAAAATCAATACCGCAATAAAAAAGTTGCATTACTTTACTAATTTTAACTGCTAAATTTTCTTGTTCCTTATTCAAAAAAAAGCGTTCTGCCTTTGCCCCTTTATTGAGATTAGAACGAAAATCACTATTATTAGTTGTATGTAAAGCACCTATAACTTTATTGTTCACAACAATAACTCTTACCGATTCACCTTTAGAGCAAGTAATGTATTTTTGCACAATTGCTTGTTGATTAAAAGACATTAAAGTTTTTCTTAATTTTTGTTCATCTAAACACAAAAAAACATAATCACCAAAAGAACCAAAAACACTTTTAACAATAACAGGAAACTCCATACTTTGTTTTATTTGATTAATAAAAACATCGAGCATTACTGGATTTCACTCCCTGTCAAAATTTTGAGGTCCTAAAAGTGTTTTAATCTGCTTTATGAATGGATATTGTGCAATGATAGCGTGACTAAGTCCTTTATTATCTGCATTGTTAATTCCAATTGAACTGTTAATTACTTCAAAACCATTGCTTTCTAGTCATCTAGCTAAAGCAATATTTTTATCCATAAAAAGAATTTTGTTGGATATGAAGGGTAAATTTGCTTTTGCTTTAGTTGTATTAATATCAAAACTAGTAAAGAATTTTAGTTCTATATCTAACTCTTCACTAGCATTTTTTAAAAGTTGAGTTTGATTTAATTTAGAGCTAATTTGATTAAATGCTGGATTGTAAACAACGTTTATTTTTTTCATGCAATATTCAAAGATTTTTTATTATTGCTAAAACTGTAAAAGGGCCAACACCGCCAGGCGTTTTAGTTCATCATCTAGCTTTTTTTTCAATGCCTTCAGGATTTATATCACCACAAAGTTTATTTTGCTTGTTTCTTGAAACACCTATATCAAATAAAATAACTCCTTTTTTAAAGTTTTTAGCTTGAAAAAAATGAGATTTTCCAATAGCAGTAAAAACTATATCAGCACTTTTAATCAATTCAAATGTATTTGGAGTATCTTTATCGCATGCTTGAACTTTATAACCACTAGTTTTTAAAAATTCATAAATAGGTTTGCCACCAGTTATTCCCAAACCTACCACAACAATTTTTTGATTATTAAATTCAAGGTTAAATAGTTCTTTTAGTGTTATTATAGCTTCAACTATACAAGGTTTAATTTCAGCCAAATGATTAGTTGTTAAACCATCAACATCTTTTAAAGGATTAATTGCTTCTAGTAGTGAGTTTTTATCTAAGTGTGGATAAACTGGTAATTGCAAAATGATACCAGTAACATTTACATCATGATTTAAACTAATTATTTTTTCTATTAACTCTGCTTGAGTTATTTGATTAGATAGTTTAATTAATTCACTTTTTGCTCCTACAGTGTTACAAGCAATAAGTTTTTGTTTAATAAAACTGTCTGATGAATCATCATCATTAGCTTGAATAATCACTAACTTACATTTTGATCAATCATGCTTCTTGTAAGTTTCTAAGATTGATTGCGCTTTTAGTTTTCCATCAAATGACATCTTATTTTGCCTAATATAAAACAAAACGTCTAACTTCTTATTTATGCAAAGGTAAGTTAGACGTTGGAATTCTTTAATGGTGGAGATGAAGGGTCTCAAACCCTCAACCTCCTGAGTGCAAATCAGGTGCTCTATCAGTTGAGCTACATCCCCATTATTGGTGGAAGTAAATGGACTTGAACCATCGACCTCACCCTTATCAGGGGTGTGCTCTAACCAACTGAGCTATACTTCCAAGCATAATCCTAAGGGTATTTAACTAATTATTATAACAATTTTAATTTAACCAAAATACCCCTCGAATTTTAACAGTTTTTATAATCAAAACAGCTAATTTTATGGGGCTTGTTTTAAAAGAATTTAACAATAAAATAAGAACAGCATTAATTCTCGCTCCTTTTTTCACTTTCGCTCAGATAGTGATTGATTTAATCATTCCTTCTTTTTTAGCAAGTGCTATTTCAGTTGTGTTTTCAATTGATAAATTAAAACAGGATGAGTCAGGCGGAAAGACAATTTCAGTTGATTTTATTGGTGGGGCTAATATTAATTTTGCTAATGTAAGAGAAGCGCAAATAGTTTTAGCAACCACAGTTATTTTACTTGCATTATGTGGACTTTTTTTTGGTCTAATATCTATTTATTGTGCAAGTTATGTTTCAGCAAACACTTCTTATCTATTAAGAAAAAAGATCTTTGCAAAATTAATGCGAATCACAACCCCATCACATGACCATTATGGTTCTTCTACTTTATTAGTTAGATTAACAAATGATGTTTATTTGATGGAGGTAATTGCTTTTGATTTTTTAAGGCTAATTATTCGTGCTCCGCTTTTGTTTATAGGTGGATTAGTGTTTGCAGTAACTACAAATCAAGATATGTCAATATCTTTACTAATTACTTTTCCTTTGATTCTTTTGGTAATTGGTATTCTAAACCGTAAATCTATTCCTTTATTTAAAGAAAATCAAAAATCAGTTGATAAGATAAATGAAAGAGTAGAAGAAGATGTTTCAGGTTATAAAGTAATTCAATCTTTTAATCTTCATTCCTTTACAAATAACAAATTTAAGATTGCAAATGAAGGATGAAAAAAGAATAGTACAAGTTCTTTATTTATAAACTCACTTAACATTCCATTTACCTTTTTTTTAAGCAGTTTAACTATTATTATTGCTCTACTATTAGTTTTTCAATTAGATAGTAGTGTTTCAGTTGACCCATTACCGCAGGATGCAGCTATTAGACCTAATATCTTTGCTTTTTTCCAATATAACTTTTACATTGTTTTAGGGCTTATCTTAACCTCTTTAACAATGGTGAACTTTAATCGTTCTAGAGTTGCACTAGGCAGAATTAAAGATATTTTATCTCAGCCTGAAATAAAAACTATTACAAATAAAGATCAGAAAGAATTATTACCTACCTTGGAGTTTAGAAATATCTCTTTTGGTCTTGGCAATAAGAACAATAACAATTTTTTACAAAATCTTAGTTTTAAATTTGAAGCATATAAAACATATGGTATTGTTGGGCCTACAGGCTCTGGAAAATCTTTAATTGCTAACATTATCAGTGGTTTATATGAGCCTAATGAGGGTGAAATTCTCATTGGTGGGGAGAAAATTCAATCTATTGACAGTTTATATCTATCAGAAATGATAGGAATTGTTTTCCAACAAAACATCCTTTTTAAAGGAACAATTTCTTCTAATATAAAAATTGGTATTGAAACTAGAAGTGATTGAAAGAATCAATCTGATTTACAAAAGAATGAAGCGATGAAAAACGCAGCTAAAATAGCTTGCGCTGACACCTTTATTGAAAAGTTTTCTGATAGTTATGATCACAATGTTGAACAGTTAGGTAAAAACTTATCTGGTGGACAAAAACAAAGAGTTGCTATTGCAAGAACTTTAATTACAAAACCAAGAATTTTAGTTTTTGATGATAGTATGAGTGCTCTTGATGCTCTAACTGAAAAAAAAGTAAGAGAAAATATTGAAAATGATTTAAAGCTAACTACCAAAATCATTATTAGTCAAAACATTAATTCAATTAAACACGCAGATAAAATTTTGGTAATTGATAATGGCAGAATTGTTGGTTTTGATAGTGATCAAAAACTAATGAAAAATTGTTCACTTTATCAAAAGATGAAAGAATCTCAAAAAGATTTGGGAGGTGATTTTGATGCAGTTAACTAGTGTAAAACCAAGTTCTTGAAAGATTTTAAAGAGATTAATTACTTCAATGGAAGGAAGTTGAAGTTATAAATTACTTTATGTTTTTTTGTGTATAGTTCTTGGTATTTTATATGGAATTGCTAACCCTATCTTATTAGCACAAGGTCTTGGTTTTATTTTTCCTATTACTAATAGTAATGGTCGTGCTGTTGACTCAATATATTCATTAATTTACCCAACAAATTTAAATGTATTCATTAGGCTCACAATTGTGAGCGTAACTGTTTTTATAGCTTATGCATTAATCTTTGTATTTAATGTAGCGCAAAACTATGTAGGGATTAAACTTTACCAGCAAACATGTGCTACTTTGCGTTGAAAGGCATATTTAAAAATGCAGAGTATGTCAACCAGCTTTTTTGATACGCAAAATAATGGTGATCTTATGAGTAGGTTAACTAATGATATGTATAACATTGATAACCTATTCACTCAAGCTGGTGGACAAGCTATTCAAAGTTTGTTTAATATTTTAACAACCTCAGTATTAATATTTTTATTAAGCCCAGTTATTGCACTTATTTCACTTTCAATTTTAGCTACATTAATTACTTTTTCTTTTGCCTTTCTAAAGAAATCAAAAACTTCATATAGTCAAGTACAAAATAATTTGGGTGATATGTCTGGTTATATTGAAGAGGTTTTAACTAATCATAAGGTTGTTCATGTCTTGAAGTTGCAAGAGATAATGATTAAGGATTTTGATCAATACAACAAATCAATGATCAAACCAACTGTAAGAGGGAATACATATTCGATCTTTCTTTTTTCTTGGTTTGGTTTTATATCAAATATTACTTATCTGGTTTCTATATCAATTGCTACTGCTTTTAGTGTTAATTCTATTCCTTCATTTGGAATTAGTGTTATTAACTATTCATTCATGTTGTCTTACATTGCTTCTTTAAGACAAATAACTTTAGCATTAGATCAAATCTTTACCCTTTGAAACTTAGTTCAATTAGGGGTTGTTAGTGCAGAAAGAGTATTTAAGGTATTAGATCTTAATGTAGAGAAAGATACTGCTACTATTGACAAATTACCTGATATTAAAGGTAATATAAGGTTTGAAAATGTAGCATTTGGTTACAATAAAGATAAACCTACTTTAACAGGAATTAACTTTAGTGTTAAACATGGAGATGTAGTTGCAATAGTAGGTCCTACAGGAGCTGGTAAATCAACTATTATTAATCTATTGATGAAATTCTATAAACCTTTTGAAGGAAAGATTTATATGGATAACTTTGAAATTAGTGATGTAACTAAAAAAGCATGAAGAGAAAAGATTTCTATAGTATTACAAGATTCATTCTTATTTAGCGGCACAATTAAAGAAAATATTCGTTTAGGCAGACAGGATGCTACTGATGATGAGATTATCGCTGCATGTAAAACTGCTAATGCTCATGATTTCATCATGCGTTTACCAAAAGGATATGACACTTATATTTCCAATAAAGCAGATTATCTTTCTGTTGGTGAAAGGCAATTATTAACAATTGCCAGAGCAGTAATCCGTAATGCTCCAGTTTTGCTCTTAGATGAAGCAACTAGTTCAGTTGATGTCCATTCAGAAAAATTAATTCAAGAATCAATAGGAAGGTTAATGAAAAATAAAACTTCTTTTATAATTTCTCATCGTCTTTCAATTATTCGTGATGCAACATTAATAATGGTTATTAATGATGGTAAAGTACTTGAAATGGGTAATCAGAATCAGCTGATGAAACAAAATGGATTTTATGCACGTTTAAAACAATCTTCGGTTCGTTAATAAATTCTAATGACTGTTGCTGAAATTAAAAAACTTGCATTAAATAATCAGGTATTTAATGAAGCAAAAGCGCTTTTAGAAAAAGGTAATGTTATTTTTCCAAAAAAATTCTTAAAGCGAAAGAAGATCATTATTGAAGTATTAGATGGTAAGGTTTTTAAGGTTCAAATTAATTTAAAAACCGCTGCTGCACACTTGGATTGTAGTTGCTCTAATGATAAGCAAAATTGCGTTCATATAATTGCAGCGCTTTTGAAATATAATGATCTAAAAAATCAAGATAACAAAGAATTTGACCTTAATAAAGCCGATAAATTAGAGTGCAAAGAAGTTGAAATTCTAATTGAAAATGTTAGCTTAGCAATTGTTAATGGCAGCTGAAAATTAAAGATAGGTTTTGTAATTAATATTGATAAAGTTCAAACTAATACAACTGCTTTACGTTTTTATTGTTGTGATAATAAAGATGTTTATTTTCTACATACTGAAGATGAACAACTTTTCAGGATTGCTTTAGATAAATTTAATAGTGTTGAAAGACAAACATTATTAATTTTTGATCAGCTAAATAAAACAAAGCAAATGCAATATGAAAATAACAGTTTGTTTTTTAATTTAGATCAATTCCTAAGCCTAGTTAAGGAAGTTAAAAAACCTTCATTATTCTTATTAAATGAGGATAAAACTGACAATATTCTTTTCTTAAGAAGTCAACATAAAATCAATGGATTAAGCCACGTTTGTGGTTTTTTAAATAATAAGGTTTTTGATTTTGTATCCTACAATGAAAAAACTAAGCAAATTGTTTTACGCTTAGCTTATCTTAATAAGTTCACTGATTTTAAATTTCCATACAACATTAACATCTATAAACTTGCTTTTGGAGAAACATTATTTTTCCATTTTTTAATCCATTTAAAAATGAATGGTTTTAAAAACATCTTTTTTCAAAGTGATGTTGCTATTGTTAAGGAAAGTGAATATCTACCTAAGATGTTTTTGACTATTGAATTTAATACACAAAAAAACAAGTTTATAACTGATGCTTTTTTTAAATATAAGAATAAAAATAGCAATACTTTAACAACTGTTTACCCCCATCGCTATTACTTAGCTCAAAAAACTAATACATCAAACTTCAACCGTTTACTTTTTTATGAACAAGCACTACAAAGGTTTTATGAAGAATTATTTCAAATAGATTATTTAAGAAGATTTGAAAACATTCCCATTAAAGATAAGAATCAAATTGCGCTTTTTAAAACTGTTTTTGATGATTACAAAACCATTGATTTAGCAGAATTGAAACTTACAAGTAATCTCTTAAATTACAAACAGTTACATTTTTCAATAAGTGATATTAAAGCTTTAAAAATTGAAGATAGACAACTAAAAATTGAATTTAAAGCTGGCGGGATAGATCTAAAGTTAATTAAAAGTGTTTTAAGTAACTACTATAAGGGTAATGCTATTTGTATTGGTGAAGATGGTTGATATGATTTGAACGATGAAAATGCTAAAGCACTAATTTCATTTTGGAGTCAAATTGACTTAAGAAATGCTACTTGTGATGCTAATAACAATTTGCTTCTTGCTAAATACCACTTGTTTGAAGTTGTAGATACTATTAGTAAATACACTGATGTAACTAATTTATTAGATGAAAAAACAGCCTTACAATTAAAAATAGCTAGTGAAAATCAATTTCATCTTTCGTTAGATAATAACCAAATTAATAACTTACGTAAATATCAAAAAGAAGGAGTTAAATGGATAAGGGCATTAGAAGATAATCAGTTTGGTGGAATTCTAGCAGATGAAATGGGGTTAGGTAAAACTGCTCAAGTGATCTTTGCAATGCTGGATAGTTATCAATCAACAAAATCACTTTTACCTAGTTTAATTATCGTTCCAGCATCCTTACTCTTAAATTGAAAAAGTGAGTTCCAAAAGTTTGCGCCTCATGTGAAAATAGTTACTGCTAATGGCAATTTTAAAGAACGCTCACAGATATATGAATCATTAAAAAATCAGATTTTGTTAATGAGTTTTAATGTCTTGAGAAGTGATATTAAATGAATATCTCAAAAAAAGTTCCATTATGTAGTAATTGATGAAGCACAAGGAATAAAAAATGAAAATTCTACTGTAACAAAAGCCGCTAAAAAAATTAAAGGTAATTTTTGCTTAGCACTTACCGGTACTCCTATTGAAAATCGTTTGCTTGATCTTTGATCTTGTTTTGATTTTGTTTTACCAAATTTTCTTGGTAATAAAAAACAGTTTTCAGATCAATTTGAAAAAGAAAAAAATGATGAAAGCTTTCAAAAATTAATGAAAAAAACAAGTCCTTTTATTTTAAGAAGGACTAAAAATAAAGTTTTAAAAGAACTACCTAAAAAAATTATTACTGATATCTATGTTGAACTTAGTGAAGAACATCAAAAACTGTATGATAAACAAAAAACAGATGGTTTGAAGGAGATTAAAGAAAGTGATGCTAAAAATGCTTTAAATATCCTTAGTTTAATCTTGAAATTAAGGCATATTTGTAGCTTAGTTAAAGATAATGATGTTAATGATTTTGAAGATAATTCCAAAGCTAATACAGCTTTAAACATTATTTATGAAGCACTTGAAAATAAACGCAAAGTTATTTTGTTTACTCAATTTTTAGATGTAATTGATTGTTTTAAGCAAACTTTAAAAAATCAAAAGATTGATCACCTGGTATTTGATGGTAGAAAAACTGTGAAGAATAGAAACACTATTATCCAGAAGTTCAATAGTGCTAAAGAACCTTGTGTGATGTTAGCTTCTTTAAAAGCTGGTGGAGTTGGTATTAACTTAACTGCTGCTGAAGTTGTTATCCATTTTGATGTGTGATGAAACAGTGCTGTTGAAAATCAAGCAACTGATAGAGCACATCGTATTGGTCAAAGTAAAACTGTACAAGTTTATAGAATTATTGCTAAAAATACTATTGAAGAGCGAGTTTGTCAAGTCCAGAATCAGAAACAAGAACTTGTTAAAAAAACCTTGGTTGAGGATGTAAATTTCTTTAAATCTCTTTCACATGAAGAACTCTTAAAGCTTTTTGAATAAAGCAAGAATTATAATTAACACTCTAAGGATGCAAGTGATAAATGGCTGCTGGTAAAAGGGATTATTATGAAGTTCTAGGGATATCTAAAAACGCTAGTTCTCAAGACATAAAAAGAGCTTTTAGAAAGCTTGCAATGCAATATCACCCCGATCGTCATAAAGCAGAAAATGAAACTACTCAAAAACAAAATGAGGAAAAGTTTAAAGAGGTTAATGAAGCATACGAAGTTCTAAGTGATGAAGAAAAACGTAAGCTTTATGACCAGTTTGGTCATGAAGGGTTAAATGCTTCTGGTTTTCATGAAGCAGGGTTTAATCCTTTTGACATCTTTAATAGTGTTTTTGGTGAGGGATTTTCCTTTGGAATGGATGGTGATTCACCATTTGATTTCATTTTTAATCGTTCTAAAAAACGTCAACAACAAATTGTTGTTCCCTATAACCTTGATATTGCCTTAGTAATTGAAATTAACTTTTTTGAAATGACTAACGGTTGCAACAAAACCATCGAATATGAAAGAAAAGTTTCATGTCATAGTTGTAATGGTTTTGGCGCTGAAGGTGGGGAAAGCGGATTGGATCTTTGTAAGGATTGTAATGGCAATGGTTTTGTTATTAAAAACCAACGTTCTATCTTTGGAACCATTCAATCCCAAGTGTTGTGTTCAACTTGCAATGGACAAGGAAAACAAATTAAAGTTAAGTGCAAAACTTGTCGTTCTAACAAATACACTGTCACCAATCAAATTAAAGAGATTAATATTCCAGCAGGAATGTATAGTGGTGAAGCTTTAGTTGATGAAAGTGGTGGTAATGAATTTAAAGGTCACTATGGAAAATTAATCATTCAAGTGAATGTATTGGCAAGTAAGATTTTCAAACGTAGTGATAATAATGTTATTGCCAATGTTTTAGTAGATCCAATGGTTGCTATAGTTGGTGGGGTAATTGAACTACCTACTCTTGAAGGGATTAAAGAATTTAATATTAGACCAGGCACTAAGAGTGGCGAACAAATTGTTATTCCTAACGGTGGGATTAAATTCTCAAAGAGTTTTAAAAGAAAAGCTGGGGACTTAATCATTATTATTAGTTATGCACGTCCTTGTGAATACACTAACTTAGAATTGAAAAAATTACGTGAGTTTATCAAACCTAATCAAGAGGTTAGACAATACTTAAATACTTTAAAAAATGAATACAAAACTTAATGTAAAAGGTTATCTAAATGTTGGTGATAACCATCAACTTTATTACTGAACACAAGGAAATCCTAATGGTAAACCGGTTTTGTATATCCATGGCGGACCTGGTTCTGGTACTGATGAAGGATGTTTAAAGTATTTTGATCTTGAAACAACTTGGATTATTTTATTAGATCAAAGAGGTTGTGGTAAGAGCAAGACTAATGATATCTTTTATGAAAATAACACTGATAAATTAGTTAGTGATTTTGAAATTTTACGTCAAAAATTAAACATTAAAAGCTGAACACTCTTTGGTGGTAGTTGGGGTTCTGCACTTGCTTTAGTTTATGCAATTAAACACCCACAAGTAGTTGATAAGATCTTTTTAAGAGCACTTTTTTTAGCTAGAGAAAAAGACTGATCTGAAGCTTTAATGGGATTAGGAAAAATGTTTTATCCTTATGAACACCAACGCTTTATGGATAGTATCCCTAAAGCTTATCAGAACAGTTATGAACAAATTGTTAACTACTGTTATGATCAATTTCAAAATGGTGATGAATCAACCAAAGAAAAACTTGCTAAAGCTTGGGTGGATTGAGAATCAACATTACTTTCACCTATTAACAAAATTCATTCAACAGCAACAGACTTTAAATTAGTTGAAAAACTAGCTTTATTGGAATGTCACTATGCAGTTAATAAAAGTTTTTTGGATGAAAACTTCATTCTAGATAACATTAGTGTTCTTAAAAATAAAAGTATTTATTTGGCTCATGGTAGATTTGATTTGATCTGTCCTTTATATCAACCATTAGCATTAAAACAAGCATTCCCTGAATTACAACTTTATGTAACCAATAATGCTGGTCATAGTGGTAGTGATGCTAATAATTTAGCAACTATAAAACACCTTTTAAAAACTTACCTTTAATGAAGCGTTGTTATATTACAACCCCTATCTACTACGCATCAGGTAAGCCCCATATAGGTCATGCTTTTACCACTATTTTGGCGGATGTAATTAAGCGTTTTAAAATCCAAAACGGATATGAGGCTTTTTTGCTTGTTGGCAGTGATGAACATGGCAATAAAATAGAAAGTAAAGCTAAAAGCTTAAATTTAGATCCTAAAACATTTGTTGATATTAACGCTCAAGCTTTTAAGTTAATGTGAAAGACCCTTAATATTAGTTTTGATCACTTTATTAGAACAACTGATGAAATCCATAAACAACAAGTTCAAAAAACATTTCAAGATTTATATGACAAAAAACTAATTTATCAAAGTGAATGAAAAGGGGCATATTGTGTTGAGTGTGAACAAAATTACTTTACTTTTAACAAACAAACAATGTTATGTGAAATAGGTCATAATCTCAGTCTTGTTAAAGAACCTTGTTGATTTATTTCTTTTTCTTCTACTAAAAATTGAATTGAAACAATGATAGGCAAAAATCAACTTAACATTGTTCCTAAATCACGTGCTTCTGAATTAAAAAATAACTTTATAAACAATGGTTTAAACGATTTAGCATTAACAAGAAAAAATGTTACTTGAGGAATAAAAGTTCCTTTTGATCCAAATCAAACAATCTATGTTTGGTTTGATGCATTGTTTTCTTATATCACCAATTTAGGATTTAGAAATGGTGATCCTAATTTTATAAAGTGGTGAAATAATGACAATAAAGAAAGAGAAGTTATCCATCTTATATCACGTGAAATCACCAGATTTCACTGCATCTATTGACCGATTTTTCTACACTTACTTGATATTAAGTTACCAACCCAATTTTTATCACATGGCTGGATAGTTGATGGTGAAGGGAGAAAGATGTCAAAATCTTTAAACAACGTTATCTCTCCAGAACAATTAATTGATCAATTTGGTGTTGATGGTACAAGATATTGTTTATTAAAAGAGATGCGTTTAGATAAAGATAATCGTTGTAGTATTAGCATCTTAAAAGAGATTTATAATGCTGATCTTGCCAATAGTTTTGGAAACCATGTTTCACGTACTTTTGGCATGATTAAAAAGTATCTAAACGGCAAATTAGAATACCAAATTATTACTGATAATGCACTTCAAAAAATAATGATTTTAATAGATGAATCAATCGTTAAATTTGATCATTACTTTAACAGTTATGAATTTTATAGAGCGATTAATCTACTTTTAAAAATTGTTTTTGAATTAAGTAAATTAATTGATGATTTCAAACCATGAGAATTGTTTAAAAATCAGGAATTCTCACTTTTAAAACAACTACTTTTTACTTGTGTTAGGTGTGTGCAGGTATGCTATGTGTTGTTAACACCTATCTTAGTAAATACTGCTTCAAAAGTTTTTCATTTATTTAATTTCGCTGATGATGCCTGTAGAAAAGACCAATTAAGAGATGCAACTTTATTAAAAAAAATTATTATCTCTAATTCAATGGAAGTTTTATTTAAAAGAGTAGATTAAGAAAAGTAGTATTAGAATTTTATTGATTTATGCAATTAGAGTACCTCAATCTTATTTCTCAAGCTAAAGTTATTGCAGAAAAACAATTTAAAGCTAACCCTTTTTCTTTTGAAACAATTTGAAAAGAAGTAGTTAAACATTTCAAGATTTCAAAACAAGATGAACCAAGCTTAATTGGTCGTTTTTATCAAGATTTTCTTGAGGATCCTAACTTTGTCTATTTAGGTGATAGAAAATGAAAACTTCGTGATTTTATGAAGTTTGATGAATGGAACAAGATATCACAATCTATGTTTGTTACAAAGGAGATTTTTGAAGAAGGTTATGAAGATCTTTCCAATAAAAAAGTAGAACCTGAGGAAGGAGTTGGTGATTTCATTATGGGAAATGATGGTGATGACAATGAAACTGGCAGTGAAATAGTACAAGGTTTAATTAATGATTCATTCAGTGAGGAAAATCAATAGTAGATATGCTTGTTAACTTTAAATTGATGCTTCAAAAAGCAAAGCTAGGTAAATATGCAATCCCTCACATTAACATCAATAACTATGAATGGGCCAAAGCTGTTTTAACAGCAGCAAATCAAGCTAATAGCCCAATTATTGTTTCAGTATCTGAAGGTGCTTTAAAGTACATGTCTGGTTATAGTGTTGTTATCCCGCTTGTTAAGGGTTTAATGGAATCACTAAGTGTTAAAGTACCAGTGACATTACATTTAGATCATGGTAGTTATGATGCATGTGTCCAAGCATTACAGGCTGGATTTAGTTCAGTAATGTTTGATGGTTCACATTTACCATTTGAAGAAAATTTCAATAAATCTAAAAAGTTAATAGAGATAGCACAAAAAACAAATGCTTCTGTTGAACTTGAAGTTGGTACTATTGGTGGAGAAGAAGATGGTGTTATAGGACAAGGTGAGTTAGCTAATGTTGATGAATGTAAACAAATCGCTAGTTTAAAACCAGATGCTTTAGCAGCAGGAATTGGTAATATCCATGGTATCTATCCTAAGAATTGAAAAGGATTAAACTTTCCTTTGATTGAAACAATATCAAAAATTACTAACTTGCCCTTAGTTTTACATGGTGGCTCTGGAATCTTAGAAAATGATGTTAAAAAAGCAATTAGTTTAGGGATTTGCAAACTAAATATTAATACTGAGTGTCAATTAGCATTTGCACATGAAATTAGAAAATACATTGAATCAAATAAAGACTTGGATCTTAACAAAAAAGGTTATGATCCTAGAAAACTTTTAAAAGAACCTACTCAAGCAATTGTTGATACTTGCTTGGAAAAGATTGATTTGTGTGGTTCTAGAAATAAAGCATAGATGTTAAGTGCAGGGATAGTTGGTTTACCTAATGTTGGTAAGTCAACTTTATTTAGTGCTATTACTAATTTGCAAGTTGAAATAGCAAACTATCCTTTTGCAACTATAGAACCTAATACTGGCATTGTTAATGTTAGTGATGAGAGATTAGATAAATTAGCTAGCTTAATTAATCCTGAAAAGATAGTTTATACAACCTTTCGTTTTGTTGATATAGCAGGTCTTGTTAAAGGCGCCAGTCAAGGTCAAGGATTGGGTAATCAATTCTTAGCAAACATCCGTGAAGTGGATTTAATTTGTCATGTTGTAAGATGTTTTCAAGATAAAAAAATTGTTCATGTTAACAATACAATAGATCCTGTTTTTGATTTTGAAATTATTGTTAATGAACTAATCCAAGCTGATTTTGAATTAATAACAAACAGAATCAGCAAACTTAAAAGAAAAGCTGAATCAGGTGATAAAATCGCTAAAGAAGAGTTTGTATTACTTGAAATTGTTTTAAATGGATTAAAACAAGGTCAAATGCCCATTCAGACTCTAAGTGAAAGTGAATTGAAAACAATTAAATCACTTAATCTATTAACAGCTAAACCTATTCTAATAGTAGCCAATGTATCTGAGAATGACTTATTAAACCTTGATAATAATGAAGCTTTAAAAAAATTGAATGCTTTTCTTGATCAAAAAAAGATTCCCAAGGCAATCACAGTTTGTTCTTTAATTGAAAAAGAATTAAGCGGTTTGAAATTAGAACAACGTCAATACTTTTTGGATGAACTTGGCTTAAAAAATTATTCAGGTTTAAACCAAGTAATTCAAGCTGCATATCAAACTTTAAACCTTTGGTCTTTTTTTACTTTTGGTAAAAAAGAAGTTAGAGCATGAACATTTAAAAAGGGTTGAAATGCTCCTCAGTGTGCTGGGCAAATTCATTCTGATTTTGAAAAAGGATTTATTAAAGTTGAAGTAATTAGTTGAGATCAATTGTTTGCAATGAAATCTTTACAAGAAGCTAAAAAACAAGGTTTGATAAGATTGGAAGGCAAAAATTACTTAATAAAAGATGGTGATGTTTGTAACTTTAAATTTAACGTCACTTAAAAATTAATCTATTTTCAAAATGAGTTTTTTCAACATTTATTAATTAAAAATTAATGCTTTTTAAAGTTGAATCTTAATTCATTTTTCAATACTTTTTTAGGCTTCCTATTCTTTAATTAAATTAATACGCTTTTCTAAAAGTAAAAGTTAAGAAAAGTACTTTTATTCAAACAAAAACGTTTTTATAATTTTAGAGATAGTTGTGCCAAGTAAGTATTTATTCACCGTTATAATTCCAACATACAATTGTTGTCAATACATAAAAAAGGCATTAGATTCATTGCTTTTACAGAATGAATATTTTTTAAAAACACAAGTACTAATAGTTAACGATGGTTCACTAGACAATACTAAGGAAGTTGTTAGTGACTATTTAATAAAGTACTCCAATATTAGTTATTTTGAAAAAACTAATGGTAATTGGGGCAGTGTTATTAACTATGTTAAAAAAAATAAATTAGCCTTAGGTCAATATATTACTGTTTTAGATAGCGATGATTATTTTTTAAAAGATAGTTTTAAAAAAGTGGCTCGTTTCTTTGGCCATGACATGATCATTGGCGCTTTTTATTGTTATATTAATGAAAATAAAACTCGTTTTTTAAAACCTTATTTCGGTAAGACTGGTGTTATTAAAGAACATACCAAATTAAGAACCCCCCATTCCCAACCTATTGCTAAATTTTACAGTAATAAACTTTTCTATGAACTACATGATCTTAAGGAAAAGTTATTCTTCCAGGATTGTTTAATGTATCACGATGCTATTAACAGAGTTGAAAATGTTTTTTATTTGCGAGAGCCTTTAGCAGTTTGGTTTTCTACAAGACCTGGCAATTCTACAACAACTTCTTGAGAAAATCCAAATAAATTTAATGCTTGGTGTGAAATTCTCCAAAAAATGAATTTGTATGGAGCTGGGATAGTAATCTACATCTATACTATGCTACCTGGATTTCTAAAACAACTAAAGAAAAAAGAACTAATACTGAATTTGAACCATAAACCAGCTTACACTTGATTACCTAAACCTTTAGCGTTTATTTTTGGTGGTTTAATGGCATTTAAAACCAGAAAATACATTAAATATCCTAAGTAATTTATGGCAGAAATGATAGAAGCAAAAAATCTTCGTAATGGGCAAACCATCTTCGGTCCTAACAAAGAGATTTTATTAGTACTGGAAAATACATTTAACAAAACCGCAATGCGCCAGGGAATTGTTAAAACTAAAGTTAAAAACTTAAGAACTGGGGCTATTGTTTGGCTTGAATTTACTGGTGACAAATTAGAACAAGTAATTATTGATAAGAAAAAAATGAATTTCTTATACAAAGATGGTAATAACTTTGTTTTTATGGATCAAAAAGACTACAGTCAGATTGAGATTAATGAAAAAAAATTAGAGTGGGAAAAAAATTTCATTACTGAAGAAATTGAAGTTACTGTTATTACTTATCAAGATGAAATTCTAGGAGTTAATTTACCTGATTTAGTTCCTATTGAAGTTGAGTTTGCTGAAGATGCTATTCAGGGCAATACTGCTAACATGGCAAGAAAAAAAGCACGCCTTGTAACTGGTTATGAACTTGATGTACCCCAATTTATTAATACTGGTGATAAGATTGTAATTGCCACTATTGATGGCAATTACCGTGAAAGGTTTAACAAATAAATTAACTAGAACCCAAAGACGAATTGCTGTAGTGGAATTTATCTTTTCTCTCCTCTTTTTTTTACCTAAAGAAGCTGAAGTTATTCAAGCAGATTTTTTAGAATATGATACTAAAGAACGACAACTAAATGAATGACAAAAATTAATTGTTAAAGCATTTAGTGAAAATATCTTCTCTTTTCAAAAGAAAATTGAAGAACAACAATTGAAAAATCAATTAGAAATTCAAACTAAATACAATAAAATATCAGGAAAAAAGATTGATCTTTTAACTACTGCAGTTGTTTTATGTGCACTTAGTGAACAAAAGGCACATAATACTGATAAACCACTTTTAATTAGTGAGGCATTGTTGATTATGGATCGTTATTCACAAGGTGCTGAAAAAAAACAAACTCATGCTTTATTAGATAAGCTCTTGTAATGAAACGTAACTGAAGACAACACTATAATGTTTTTTTAGCTAATCTAGTTTTAGTATTTGGCTTTGCTTTAAATATTTTGGTTGCAAAACAATCTTTAAATAACACAACGCCCCAGTTCAGGTTTTTGTTTGTAACTCCTTTTCTTGGCGTTGTCATAGGTGCTGTTCTCTATTTTTTTGATGTTAAGTGGTTTTTAATTGACTATCCATACAAGAAATTTCACTTTCAAAAAAAATGAGCAATTGTTTATTTATCAGGGGTTATTGTATTTTTTTTAAATGTTTTAATTGGAGTAGTTTTACTTGTTGTAATGGTTAATTACATTACTAACCAAATTCTTGAAAGGGAATATGAAAGATTATTTACAAATTCATTACCTTATTTATGATCAACAACAGGAACTAGTATCGTTTTAAGTCTTATTAGTATAGGAATGAGTAAAACTGCACATTTTTTTATTGATATAGAGATTTTGAAAGCCAAAAAAGGAGAACCCACTGATCCTAATAAAACTGATAATAGAGCAGTTGTTATTAATCTTGATGAGAATAAAAAGAATGAAAAAGAACAGTCCCCTCCTTCTGCAGAAATGACTAGTCTTTAATTTCACTACCAAATAACCTTTTATGAACAAGTTCATTTAAAGTGTTATCTTTTAAAAAAGTGCACACAATAACTGTTGCAAATTCCAAAGCACAACCAGCACTTCTTGCTGTTATTAATTTATTTGCTACTGTTACTTTTGCTGTTGATCTGTTTTCAGACATAACTAAATTTGGGTTTGGAAAAGAAGAATAGATCTCATCTTTAGTGATAATGCCATGTTTAAATAGAACATTCGGTGTGTCACAAATAGCAAAAAGATAAAGGTTATTTACTTTGAAATAATTAATTGTTTTAAGCAGTTTTTCATCTTTATCTAAGTGTTTTGTAGCTCCTATTCCACCGGGAATATAAACTGCATCAAATTCTGATAAGTTAATGGTGTTGACAATATTGTTAACTTCAACAACACCATTACTAGCAGTTATCTTTTTTAATGAATGGTGATAACATGTTGTTTGTATTGTTTTAATGAAACTAAATACAACCATTACATTGGTATATTCAACATCATTCATCTCTGGATAGACAATAACAAGTATCTTTTTCATTTTATTTAGTACCAAATAAACGGTCCCCAGCATCACCAAGACCAGGGATTATGTATCTATTGTCATTTAACTTTTCATCAATTGCTGCAAGAAATATATCAACATCAGGATGCATTTTTTCTACTTTATTAATTCCTTCAGGTGCTGCTACTATAGCAATAACACTAATTTTGATAGGTTTATCTTCTTTAATAGATTTAATAGCAGTTAACAATGTAGTTCCAGTAGCAAGCATAGGATCAAGAATAATAACATGTGAATCAGAGATGTTTTCAGGCATCTTTTTATAGTATGAAATTACACTGGTTGTTTGGGTTTGACGATAGATTCCTAAATGACCAACTCTGATTTTATCTGAATAGCGAACAATAGCATCAATCATTCCAAGTCCAGCACGCATAATAGGTACAAGAACAATGTCATTTTTTAATTTGTAGCCCTTTGTTTTGGCAAAGGGAGTTTCAACTTCAACTATTGCTAGTGGTAGCTGTTTAGTTGCTTCAAAAAAGAGTAATGAAGTGATTTGATTCAAGGCCATGCGAAACTGGGAGGTTGTTGTATTCTTATCACGCAGTTTTGTCAATTCATTCAAGATTAAAGCATGTTGAACTTTTTTTATCACGTTAGTTATCTTTAAATAAGTTTAAACTAAAAATTAGTTGAAAAGTTTATGATCATCTTGGTGTTCAAATTCATCAAACAGTTGTAATTGTTCCATCGTTTCAACGTGTTTTTTAGTAATTTTTGTTCTTTTTTTGAAATCATTTAAGGAAGCAAATGGTTTTTCATTTCTTGCTTCTACAATTGAAGAAGCTACTGCTTCTCCCATTCCTGGAATAGTAATGAAAGGCGCAATTAAAACACCATTATGTTCAACAAACATCCTTGCATTAGAGTTTTGAATAGAGATTTGTTGCAACTTTATATTGCGTGCCATCATTTCCAAATAAACTTCATAACTTGTTAAAAGCTCCGCTTCTTTTGGTTTAATCTTTTTTATTCTGTAAAGCTTATTAAGTTCATCTAAACGGTTTTTAATAAATTCATACCCTTTTTCAAAACCATTAATATCATGTTCCTTTAGTTTGAAACTTAATAAACAAGCATAGTAACTCAAAGGGTGATAAAGCTTAAATCAAGCAATTCTTCAAGCCATTAAAACATAAGCTGCAGCATGTGCCTTAGGGAATAGATAATTAATTTTTAAACAAGCATTAATCCAATATTGTTCAACACCACAGTTTTGCATCAGGCTAACTTCTTTAGCATTTACTTTAATACCTTTTCTTACTTTTTCCATGATTTCAAAAGCATCTTTTGCTTGCATCCCTTTATTAATTAGATAAAGCATGATGTCATCTCTACAAGCAATTACATCTCTTAAAGTTAACCTATTGCTTTTAATTAGTTTTTGTGCATTATCTGCTCACACGTTTTTACCATGGCTTAATCCTGAAACTCTAATTAAGTCAGCAAAGTCTTTAGGTTTTGTCTGTTCTAAAATTTTTCTAACAAATTTAGTCCCAAATTCAGGGATACCAACCGCTCCTGTAACTTCATCAACAATGCCTGGTTTTAGGTTTAATGGTTTGTTTGAAGAAAACATCGAAATTAAGTTCTTATCAAAATGAGGGATATTTTGGGGATTTATCTTAGTTAGATCAGCTAAGTGCTTTAACATTGTTGGATCATCTTGACCTAAGATGTCAAGTTTCAATATGGCATCACCTAATGCATCATATTCAAAATGGGTGGTCTTTCATTCACTTTCAACATCATCTGCTGGAAAACCACAGGGAGTAAATTCATAAACAGAATGATCACTAGGAAAGATCATAATTCCCCCTGGATGTTGCCCTGTTGTTCTCTTAATTCCTATAAGCTTTTGTTTAAATCTTTCAATTTCAGCAGTAGTTGCAAGATCAACACGTTTAATGATTTCAAAATAGTTTCTAGCATAACCATAAGCAGTTTTTTCAGCAACTGTTGCAATTGTTCCTGCCCTGAAGGTGTTATTAACTCCAAATAATTTTCTGACATAATCATGGGCTTTAGCTTGATATTCGCTAGAAAAATTAAGATCGATATCAGGAATTTTATCACCAGAAAAACCCATAAAAGTAGCAAAAGGAATGTTATGACCATCACCTTTGAAACTTGCTTTTTCATGGCATTTAGGACAATCACGAATCATCAAATCATATCCATCATCAACACTGTCACTAACTTCAAAGTAATGACATTGTTCACAAAGATAATGAGCAGCTAATGGATTGATTTCTGATATACCAATTAAATTAGCAATTAAAGAAGAGCCTATAGAACCACGTGGACCTACAAAATAACCATCTTTAACAGATTGTTCTACTAATAGATGGGAAATTCAAAAGACAATTCCAAAACCATTACTAATGATTGCATTTAGTTCTTTTTCAATCCTTTCCTTAATAAGTTTAGGTAAATTTTTACCATACCTTTTTTCAGCTTGTTTTCAAGTTTTATCAATTAATTTTTGGTTAGAATCTTGCATCACTGGTGGATAAAGCTTGTTTTTAGTTGGTACTAATTCATTTAAGTCAAGTAGCTTCACTATCTTATTAGTGTTTTCAACGACTAATTTATAGGCAATCTCTTCTCCTAAAAAACTCATTCTTTTTAGCATCTCATCAGTGGTGTGAAGAAATACTTCAGGAACGGTTTGTTCTTTTTCTTTATTGTTAAAGTGTCTATGTCATTTCCCACCTAAACCCTTAGCACAAACTATTGCCTTATAATACTCATTTTCCCAAGGATGGATAAAGTAAGCATCAGATGCAACAGCAACTAATTTCTTAAGTTTTGTTGCTGTTTTGATAACTAGTTTGATTGCATCATTAATAAGTTCTTTTTTCAGTCCTTCACGTAGTGTATAACCAAGGTAAGCATTGGGTTGTGAAATAAGCACAAAGTCAACTTTTTCAATTGCTTTTTCAAGTTCATTAATTGGTTTTGTTAATGCAGCTTTAAAGATGTCACCCTGAACAGGGTTTTCTGTTAATAAAAAAGATTTTCTAAATTTAGCTAAGCTGCTAGCTAAAACTAATGGTCTGTTAGCATTATGATCTGTTAATGCAATTGATAACATCTCATATAGGTTTTGAAAGCCCCTTTGGTTTTTTACATAAACGATTGCTGTGTTTGTAAATACCCTTTTCATCAGATCAATTTGACACTTTTTATTTAGGTTTTGATCTATCTCTGTTAAGGTATTAATACCCATTTCTTTTAACTGCTTTTTAAAGTAAAAGAAAACTTTTTTTAAAGCTTCTGTATCATATTCAGCACGGTGTAATCTTTCATCATCAAATTCAAGTTTTAGTTTAGAACAAATATTGCTCAAAGTATGGGATGAAAACAAGGGATTCAATGCTCATGATAAACATAAGGTATCAATTAACGGGTTTGTTAATGGTTTGATGTTGTATTTTTCAAATTGAGTTTGCAAAAAGGGTAAATCAAAATTAATACCATTATGAGATACCATAACACAACCATCTAGATAATTTCTTATCTTTTCTAAACCTTGCTGTTGATCAATACCGCCTTCAAGCATCTCATCAGTTATTTTGGTGATTTCAGTGATTGTTTTTGGGATAGGTTTGTCAATTTTTAAAAAGAATTGTTGATGATCTATCTCGCTGTTATTCTTAATTTTGCGTGCTGAAAACTCAATAACATCATCATACCTACCATGTAATCCAGTGGTTTCAATATCAAAAATAACAAAGGTGGCATCACTTAACTTAGTGTTATCTGGATTGTGAACAATTTTGATGTGATCATCAGTTAAGTTGAATTCCAAACCATAGATAGCTTTTAAATCATATTTTTTAGCCACTTCATAAAACTTGGGATAAATATGGATATTATCTTTATCTGTAACAGTTATTGCTTTTCAACCTCTTTCTTTAGCAAACTGTGCATATTCTTCAATGTCATTGATGCCATCAAAAGCAGTCATTTTAGTATGAAAAACTAACTCTACTCGCTTTTGTTTTGATAAATCTAACCTTTTATAGTTATTAGGTATTTCAACAGGATTTATCTCTCTAACAATTCCATATAAGATTTGGGTTTTAGGGTCACGTTCTACTTGGATATGGGCTTTTATCCAATTGCCAATAGTAATTCCTTCAATCTTTTTTTCATCAGTATAGGATCATTTCAAAATTAACGATCCACCTAATTGAAAATCAGTTACATAGATATTAAGTGTTTTTTTACCAGTTAAACTTTCATGGGTTTTTAATTCAAAGATTTGCCCAATAATCTTGACATCATCGATCTGTTGGTTAATCTTGTGGATAGGGATAAATTCTGTTGCAAATACCTTTGTTTCAAACTGCTTATCATATTGAGAAACAGTTACAGCTTTTACTTTTAATTTAGATTCATTTTTATCAATAGGATAAAGTGCAATGAAGTTAAAGTTTTTAAAACCAGCATTATTCATCCAAAAAATGAAAGATTTACTTTTTTGAACTAATCATTCAGTTAATTCTTTGGTTTGACATTGTGCTTTTAATTCGCCATTTTCATAACTAAGAAAATTGGGATTACTTAACTCTTGTTCAATGAGAATTTTGTATTTCTTATCTTTTGAAAAGAAACTGTGAAAATAGTCTTTAATAATAGCTATCGTTACAGAAGAAAAACTATTTTTTGATTTAAAGAATGGCTCATTCTCCTTAAAGGTAATGGTAAGTTTAAAAGATTCATTATCAGCTTTAAATCCCTCATTTAGTTCATTAAATCCTTCATATAAAGCATTTCAAATATCAATAGTTAAAGGGCGACTAATAGTTATTGCAAATAAAAAAACCTTCTCTTCAATATATTTATTAAAGTAAATTGATTCAATCCTTTCAATTAAGCTGTTAAGCTCATTGTGGTCAAGGATATTGTTATCTATCAAAAAATTGGAGAGTGCAAGAACCTTTTTATCTTTTTCATTCTCAAGATTAAATACCATAAGCGTTTATTAAAAAAATCAAAATAAAAGATCGAAATTTATCAATAATTATTTTTAGTTTTAGCCACTTTTTCGTAAAAAAAAAAAAAAAAAGCAGTTTTTGCTATTTTAGACTAAAAGTGGTTATATACCGAAATTTAAACCTTCTTTTGGTTAGTTCTTACTAACAGTTTTAAATGTTATACAAGTTTTTCAAAGTGTGAATTTAAAAAACTTTAATATAGTTGTTTATTCTGTATCTAATTAACCTTTAATCTTTCAATAATGAAAACAATTCATAAACTATTTTTAGGTCTTTGTTTACCCGCAACATTAGGTCCTTTACTTGGAATTGTTGTTACAAATACTGACCAAAGTATTAAGTTTACAAGCAAATCGAATTCGATTAATAAGAATAACCAAAATAAAGAGTTGGCTCTACTTAGAGATAATTTGATGAACGAAGCGAAAGTTGATGAACCACTTTTCTTTGAAAAACGGTTTGAAAACTTTAAAAATAAGTATAGTGATATACATAGCTTAAATAACAGTGTTTTTTCACTTCATGACGTATGACTTATTAGGTGGATTTAAACAATCATTGACAACATTTTTTGATGAAGTGATTGCTCAACAACAAAAGATCAAGGATGCAGATAAGATCTTTCCAAGTACTAAAGATAATCCACCTAAAGAAGAAAATCCTAATGTTTTAGATACACTAGCTAACTACCAAGGAGCAGGATTTTTCCCTAGTTTAGGTAAAAATGGTTTTAATTTACCTGAAGCAGTGTTCCAAAATTTCACTGATTTTAGGATTAATGACTACAAGATTAAAAACTTTAATGTTGATCTTGTTAGTGAAAATGACATTATTAAACATGATAAAGTTCGTTATGCTTTTGAAGTTAAGTTCAATATTGCTTTAGTTTTATCTATTAATAAGTCAAATGTTGATTTTGACTTTGATTTCATTTTAAAGACTGATAATTTCTCAGACATTGAAAACTTTAATGAAATTTTCAACAGAAAACCTGCTTTACAATTTCGTTTTTATACCAAGATCAATGTACATAAGTTAAGTTTCAATGGTAGTGATTCCACTTACATTGCCAATATCTTGTTACAAGATCAGTTCAACCTATTAGAAATTGATTTAAATAAATCTATTTATGCATTAGATCTTGAAAATGCTAAAGAACGCTTTGATAATGAATTTGTTCAACCTCTTTATCAAAAACGACGTGAAGCTAAGCTTGCTTGAGAAGAAGAACAGAGACGCATTGCTGAAGAACAACGTAGACAAGAAGAGGAGAGAGCTAGAATCTTAAAAGAGTTAAAAGAAAAAGCTGAGAAAGATAAAAGAGTTAAAGAAGCACAAAACAACCTTCAAAAAGCACTTGGTAACTTAGATACTTTCTTTAACTTCTTTAGCAGTGGTCAAGATAGAGTTTTACTTGGTTTTGATCCAAATAAATACAATGTGCAAACCCGTGAAGGTTTGTTTAAAGCATTACAAATTTCCTATTCTAACTTCAAAACTTGAACATTCTATATCTCCTTGTTGGGGTGAAAAGAAGGTAGTGTTAAACTGTTGAAAAAACCTATCTGAAATGCCTTGAGAGATGATAAAGCATTTCAATATGCTTTTGGTTTAGGTCCAAATACTTCTGAACAACAACTTGGTAGAGTAACCCTACCTGGTTATGGTTATGAAGGAATTAGAATGAGTGATTGGTTGAGATGAGCATTGGGTTACTATACTAGTTTCACTTTAAGTCCACCTAAAAATGTTGAAGCTAATCTTATAGGTGATGCTAATGATGAAAAACACATTTGAATCTCACCTCATACTTTCAAATTAAACAGAGAGTATGGTGATGGTGAAAGATTCAAAGGTAAAGCATATCGTTTTAAACTATCAATAAGAGTTTTGAACTAGAAGGTCATTTAACTGCCCACTGATGAACAATTGGCTTTAGAGGTAGTATTCCTGGAAGCTGAAGTGGTAAGTTAAGAGTTACCCATGAGTTTGATGGTGATGTGCCTTACTATAGATTACATACAACTCCACCACAATATCGTTTAACTGATGATATGAAATTATTGTTTGTTCCGCACAGTATCCAAAGGGTAACTGCAGTTGGTAATGAAAGCATTAATGGTCTTCTCAGATCACAAAACCTTCATAACTTGGAACGTCAATCATATGAAGCGACTGCTCCTATTGATTTAATATCATATATGCTTTATGCAATTAGTGATAAAAAACCACCGCAAAAATAAGTTTTATATCACGTTTAAATAAATATTATTGCTAAGATTACTTTAAACTTGTTAGCAAACTTTAATCATTTTTTAGCTAAATTAAACAACCAGTTAATTAGTAAACAGTTCCAGAATTAAAATCAAATATTAGTTAGTTTTTATTTTGTATTTTCAAAATTCTACTCAATTAGGATGATGGTTTCTTGCTGAATTGATAGGAACATTTATCCTAATTATCTTTGGTAATGGTGCAGTTGCCCAAGTTAATTTAAAGAAGATGGCTACAAGTGAAACAAAAGCCAAGTTTTTAACAGTTGCACTTACTTGAGGAATAGGTGTTTTATTTGGTGTTTTAACTGCTAATGCTATCTTTAAGGGTAGTGGTCATTTAAACCCTGCTATATCATTATTTTATGCAATTAATGGCAGTATCAAATCACCTACTGCATTAATATGACCTGGTTTTGTAATTGGGATTTTAGCTCAATTCTTAGGTGCAATGATAGCTCAAACAACACTTAACTTTTTATTTTGAAAACAACTATCATCAACCGATCCACAAACAGTTCTAGCAATGCATTGTACAAGTCCTAGTGTATTTAACATTCCTAGGAATTTTCTAACTGAATTTATTGCAACTTTAATATTGATAGGTGGAGTTGTTGCTGCTAGTCACTTTCTTCATAACAACCCAAACTCTGTTCCTCCTGGATTTATGGCGCTTTGATTAGTTGCTGGAATTATTATTGCTTTTGGTGGCGCTACAGGCTCTGCAATTAATCCTGCAAGGGATTTGGGAACTAGAATTGTGTTTCAATTAACTCCAATTAAAAATAAGGATGCGAATTGAAAGTACAGCTGAATTCCAGTAATTGCTCCTTTATCTGCAGGATTAGTTTTATCAATAATTATTGGGTTTTCCCCTGCACCTGTTCTTTAAAGTTTTTGGTTTTAACAGTAACGTGCTTTTTAGTTAAAAATGAGTGGTGCAGTTTGCATCTAGCTTCATAAGCTTCTGCATCACCAATTAAGACAAGGTTATCATTAGTATTTTTATTTACTAGTCTTTGGGTTCGTTGTGCTAAACTACCACAAACATTGCAAATAGCATCAAGTTTATTAACTACATCAGCTATTGCTAAAAGTTGGGGAATACAACCAAAAGGCTCAGCTCTAAAATCAGTATCAAGCCCAGAAATAATTACATTTTTTCCTATCTCATTGAGAGTTGTAACAACTTCTATAATCTCATTTGAAAAGAATTGTGCTTCATCAATGGCAACAATTTGATAGTTTTTATCAACTAAGTGATCATAGATCTCAAAAGGAGAATTAATAGTTATTGCTTGATCATATTCACCGTTGCGTGATTTGACAATATTAGTTTGTCTAGTATCAATTATTGGTTTGAAGATAACAACTGAAATTTTGGCTATCTTTCACCGTTTTATTTTGTGAAGTAATTTCTCTGTTTTTCCAGAAAACATTGGGCCACAAATAACTTCAGTTCAGCCCTTTTTGGTTTGAAAAGATGGTTGATATTTACCCATATAATTTCGAAATTATAATTAATGACACATGAACTTCTTGCAAAAACCAAGGGGAGTTAAAGATTGGTTTGGTGATGAATTAGTTTATTTTAATTGGATTGTTAAAAAAATAAGATCTTTAGCATTTAATTGGGGTTTTAGTGAAGTTAAAACTCCGTTGTTTGAAAATGCACAACTTTTTCAAAGATCTAATGCTAATGCTGATATTGTTCAAAAAGAACTATACCAGTTTTTTGATAAATCTCAAAGAGAATTAGCTTTAAGACCTGAAGCTACTACACCAATAGTAAGACTTGCTTGTGAAAACAAATTAATGCAAGAAGCAAATTTTCCCTTAAAGTTATTTTGCATTGGTTCAATGTATCGTTATGAACGTCCACAAAACAATAGGTTTCGTGAACATTGGCAATTTAGTTGCGAAGTATTTGGTTTTTCCAACCTGTTTATCTTTTTAGATACACTTTTGTTTGCTAACTCTTTGCTTGAAGCACTTGGAATTACTGGATATGTGCTTAAAATTAATAATCTTGCTAACTTTGAAACACTTAGTAAGTGAAATAAAGCCCTAAAAGATTATTTAACTCCATATAAATTAGAACTAACTGAGCTTTCTCAAAAAAGATTAGAAAAAAATCCTTTGAGAATTTTAGATGACAAGATAGATCAAAAAAAATCATTTTTTAAAAATGCTCCTAAAATTACTGATTTTTTAGATGCAAGTGCAAAACAAGATTCAGAATTGTTAAAAACACAACTAAAAAAACACAATATTAGTTTTGAATGAACAGACAATCTAGTTAGAGGATTGGATTACTATACTGGATTTGTGTTTGAATATGTAAAAAATCAAGACACAATTCTAGCAGGTGGAGTTTATGATAACTTAGTTGAAGAATTAAGTAGTAATCCAACTCCCGCATTAGGTTTTGCTTGTGGGATTGAACGGTTAATTAACTGTTTAGAAACTGATAAAAAAGCATTCATTTTGAATACTAAACCAAAGCAGATGTTAGTAATTTGCTTATTTGAAGAAGCACTTGAAGAATTGATTTGACTAGCTAAATTATGAAGGGAATATAACCAAGTAACTGTTTATCCCAAAGTTATTAAAGTTGATAATGGGATTAGATTAGCAAATCGCTTGGGTTATACTTTCATTGGCATTGTTGGAAAAACTGATTTTGACAAAAAAGCTATTACAATCAAAAACTTAGTATCTAAACAACAAACCATTCACACCTGAAATGAACTTGGAGAACGAAAAGTGTTTTAACCAACGAATTTTAATTGGCTCAATTTCAACTGAACAACTCAATAAAACAATAGTTATTATTGGGTGAATTAAACGGATTAAAAAGTTAGGTGAAATTAACTTTATTATCGTTGGTGATAAATCAGGAACTATCCAAGTAACTTGCAAAGATAAAGAACAGATTCAACAACTTACAAGAGAAGACATAGTTATTGTTAAAGCCAAATTACAACGCTTAGATAGTGTTAGATTTGAACTGATAAATCCAACTATTAAACTTTTTTCAAAGTCAAAAACTCCTCCTCTAATTATTGAAGATGAAACTGATGCTTTAGAAGAAGTTAGGTTAAAATATCGTTACCTTGATCTGAGAAGACGTGTGATGCAAAAACGATTGTTATTGCGTCATCAATTTATATTAGCAATTCGTAACTGATTCAACCAGCAGGGTTTTATTGAGATAGAAACACCTACCTTATCCAAATCAACTCCTGAGGGAGCACAAGACTTTTTAGTTCCTGCAAGAATTAGAAAAGATTGTTTTTATGCTTTAGTTCAAAGTCCACAAATCTATAAGCAGCTGTTAATGATTGCAGGAGTTGAAAAGTATTTTCAAATTGCTAGGGTCTATCGTGATGAAGATAGCAGAAAAGATCGTCAACCAGAACACACACAAATTGATTTTGAGATCTCTTTTTGTAACCAAAAAATGATTATGAATCTAGTTGAAAAACTCTTTTTTAGTGTTTTCTTAGATGTTTTTCAAATCAAAATAAAAAAGACTTTTCCTGTTTTTAAATTTTCAGAACTTTTTGAAAGATTTGGTAGCGATAAACCAGATTTACGTTATGGTTTTGAAATAAAAGATTTCACCTCACTTTTTCAAGATCATCAAAATCAGTTCACTAAATTAATTGAAGCAAAAGGCATTATTGGTGGTATTGAACTTATTAATATTGAGTTAAGTACAGACAAAATTAAAGCATTAAGAAAAATTGCTAAGGACCATGATGTGAGTTTAGAAGTTCATAATAAAAATAATTCAACATTAAAAACTTCAATTAAATGTGATGAAAAAAACACCCTTCTGTTAGTAGCAAATAAATCTAAAAAGAAGGCATGAACTGCTTTAGGAGCAATTAGAAATGAGTTGAAATACCACTTGGATATCGTCAAGCCTAACCAATACAGCTTTTGTTGAGTTGTTGATTTCCCTCTCTATGATTTTGATGAGAAAACAAATCAGTGAATATCAAATCACAACATATTTTCAAAACCTAAACAAGAATGAATTGATAATTTTGAATCAAATAAAAACGAAGCATTAAGCGAACAGTTTGATCTTGTTTTAAACGGTTTTGAAATTGGTAGTGGTTCAATAAGAATTAATGATACAATTGTTCAAAAAAGACTAATGAATTCTTTGAACATTGACCCAAATAAGTTTGCTTTTCTTCTAGAAGCTTATGAATATGGTGCTCCTGTTCATGGTGGAATGGGACTAGGTATTGATCGTTTAATGATGATTCTTAATCAAACTGATAACATCAGAGAAGTAATCGCTTTTCCTAAGAATAATCATGGTATTGAAGTCCATACAAATGCTCCTGATAAAATTGAAAAAGAGGAGGTTAAATGATGGATAAAAGAACTAGTGAAATAGAGTTTCACTTAAAGAATCTTTTGGCTTGTGATGCATATAAACTTTCACACCGTTTAATGTATCCACAAGATACACAAAACCTTTATAGTATGTTAACTGCAAGAGGTGTTTATGGTGATTTTAAGGAGTTTGTTTGAAACCATGATTTTGCTAAAGAGATCCTTTTGAATGTATTTAATGGTTTTGTAAACAGTGTAATTGAAGTTAAAAAAACAAATTGCTAGCTGCAGCATTGACAGATAAATTAGTTAGTGTTTTTAATGATCATGAATTGGCTAATGAATTCACACAACACATCTGTCATTTAGCTAGTTTCTTAGAGAAAAATAAAAAAATGCCGTTAGTTGCAAAGATCCATGAAAGTGATCAATCATTACCATTTAGAACTCCTTTAATAACTATAGAAGGAGTTGAAAATATTCCAAACAACTTTGTATGGTTAGTTAATTACTTTGAAACTGTACTTCTAGAAAACATTTGGTTGTTTCAAACTGCTTCTACAGTTGCTAAAAGAATTAAATCTTTACTTGAAAAATATGCTAAAGAAACCGCAGATGAAACAAGTTTTATTAATTTTCAATGCCACGACTTTAGTATGCGGGGCATGAGTAGTTTGCAAAGTGCTTTGTATGTTGCTAGAGCACACTTGCAATACTTTACTGGAAGTGACACGATCTTAGGTGGGGATAATTCTCGTTCAATTTTAGCTTCTGAACATTCAGTGATGTGCGCAGATGGTAGTAAACATGAATTGAAAACTTTTCAACGTTTATTGGAAAAGTTTAAAGATAAAAAACTTTCTTTAGTGATTGATTCTTATGACATGTGAAATGTCCTTGATAACATTATTCCAAGGTTAAAAAACTTAATCTTAATGCGTGGTGCTACGCTTTATTTGCGTGCTGATTCTGGTAATTATCAAACTCTTATTTGCAATCCTAATTACAAAAAGCAAGATAAAAGTACATGAGCAATGATCGATTACTTAGATCATCATTTTAGTTCAACTATAAATAAAAAAGGTTATAAGGTTTTAAACAAGAAAATTGGCATTATTTATGGTGATGGAATCACCTATCAAAAGATAGAATGGATCTTAAATTGTTTAAAAAACCATGGTTATTGTTCTTCAAACATTATTTTTGGAGTTGGTAGTAGCACTTATCAAAATTTAAACCGTGATACTTTAGGTTTTGTATACAAATTAACTGCTATTAAAAGAAATAATAGATGGATAGACGTTAAAAAAACTCCCATAACTGATCTATCTAAAAGTTCAAAAGGCGGTAGATATAAAACAAAGCGATTAATTACAGTTTATTAATCAATTTCTTTCCATTTTAAAGCACGTTCAACTGCTTTATGTCAACTGTTAATTTTGGTTTTTCTTATGTTTGGGTCCATAGTGCTTTTGAACTTTTTATCAAGAGTAGTGAGTTTTTCAAGTTGATGAATGTCTTTTCAAAATCCACAAGCAAGTCCAGCTAAAAAACAAACACCAACTGCAGTGGTTTCTTTATTTTTAGGGATAGAAACAATTACATCTGCAATATCAGCTTGAAACTGCATTAAATAGTTTGATTTAACAATCCCCCCATCAGCTTTAATGCTAGTAATCTTATAACCTATATCACTTGCCATTGCATTTAATAAATCATTAGTTTGAAAAGCAATTGACTCTAAGCTAGCTTTTACTATGTGTTCTCTTTTAGTGCTTGCTTCAATTCCTAAGATAATACCCCTAGCACTAGCATCTCATCAAGGAGCTCCAAGTCCACTGAAAGCTGGTACAAAAACTAGGTTTTGTTCATTTTCTTTTGCAAGTTCTGCATAAAAATCACTTTCCTTTTCTGAATAGATAATTTTTAATGCATCCCTTAACCATTTTATAGCCGCACCCGCTACAAACACACTACCTTCCAATGCATATACAGGTGGATGATTTTCTAGTTGTCATGCTACTGTTGTGAGCAGATTGTGCTTTGAGAGTGTTGGTTTATCACCAATGTTCATGAGTGCAAAACATCCAGTACCATAGGTATTTTTTACCATTCCAGGTTCAGTACAGAGCTGACCAAACAAAGCTGCTTGCTGGTCTCCTAAAACTGCTCTAATTGGTACAATACCTTTAGCATTACTAGATCAGTGATTAGTTTCAATATCACCAAAGTAAGCATTGGAACTCAGAACTTTAGGTAAGATTGAAACTGGTACTTCAAATAAATCACATAACTCTTTGGATCACTCCATTTTGACAATGTCAAATAAAAGAGTTCTTGAAGCATTTGAAACATCTGTAACATGCATTTTTCCATTAGTTAGTTTTCAGATTAATCAGCTATCAATGGTGCCAAATAACAACTTTTTTTGCTCCATTAGTTTCTTTGCTAAAGGAACATTTTTTAAGATTCAAGCTATCTTAGTAGCACTAAAATAGGGGTTAATAGGTAATCCAGTTTTTTGTTTTACTTTGGTTTGGATTAACTTATCCTCATTGAATTTTTGACATAGTGCTGCAGTTCTTTGATCCTGTCAAACAATGGCATTATAAACAGGCAAACCATTCTCTTTATTTCATAAAACTATTGTTTCTCTTTGATTGGTAATACCAACTGCAATCACTTCATGAGATTTGATTTGTGCTTTATTTTTAGCACTTTGCATGGTAGCTAGTTGGGCTGATCAAATTTCTAGTGGATCTTGTTCAACTCAACCACTATTAGGAAAAAAAGTGTTAAATTCGTTTTGTGCTATTGCTATTTGGTTAAGGTTGTGATCAAAAACAATTGATCGACAAGAACTAGTACCTTCATCTAAGGCAATGATGTATTGTTTTTTTAGATCCATGATAAGTTGTAATTAGGATTTCAATTTATCTTCTTTTTTAACTTTAAATTAAGGAGTTCAACTGCTCTCTTAGCAATAGCAGGACTTGATGTTAAGCCAGGGGATTTCATGCCACCAAGGATGATAAAGTTAGGATTGCTTTTTGCTGTTCTAATAACAAAGTCATTTGTTTCAATATCAATAGCTCTTGAACCTGCAAAACTATAAATACTATTTTCAAATTGCAATGAAGGAACCATTTTTTTACCAATAGTTTTAATTTGGTTAATTGAATCTAAATCTATGGAACGAGTTTTGTTTTTTTCAATTCCCTCAACTGCATTAGGTCCCACAAGAATATTACCATCTAACATCTCAGCAACAACAACTCCCTTACCATGGATAGTAGGTACCATAAAAATAATTGTGTTAATCTTAAGGTTGTTTTGATTTTTTAAAACTAGATATTGTCCTTTTCTTGTTGTTTGTTTAAAATTATCAACTTGTGTAGTTTCTGCTAGTCAATCTGCATAATGACCAGCTGCATCAATTAACTTTTTGGTTTTAAATTGTGGTGTTGTTTCATTGTTAATAAAAACTAAAAAATCATCATCAGAATCTATTTCAATTTTTGTAACTTTTTTATTTGAGTAGATGGCAACATTGTTTTGTAAACTAGCAAGGGCTAAACACTTGGTTGCAATTAAAGGATCAATTAGTCAACTACCTTCAACTTTTAAACTAGCTACTACATTTGGATTGATAAACGGTTCTTGCAATAAAGTTTGTTGTTGGTCTAATATTTGAATGTTTTCAACAGGAATTGAGTTTTTAATTCCCCTTTCTTTTAACAAATTTAATTGAAGTTTTTCTTCATTGTTGAAAGCAACAATTAAAGTAGCAATTTTTTTCCTTGGGAAGATTAGTTTTTTAAATCAATCCTCAATTCAGATTTTTCTCCCTAAGATGTTGTATTTGGCAGTTAGCTTATTAGGATTAGGATCAATTCCTGAATGGATTACACCACTGTTAGCCTGCGAAGTTTCACAACCCAAAAAAGCATTTTTTTCTAAAAGTGCAACTTTTAATTTATATTGGCTGAGTTCATATGCACAACTAGTACCGATGACACCCCCACCCACAATTAAAACGTCAATTGTTTGCATTAATTAATCTTCTAGTATTTAGTTAGTTATTTTGCAAAAAAGGATTAGTTTTATAGTTGTGATTTACTTTTTGGTATCTCTATAAAAAGTAGTATCAAACATTTTTTTATCTTTTGATTTCTTTTTACTAAAAATAATTAGCTAAAATACCGTCATATATGGGAAAGCTATTATTTGGTAAGTTAGTTTTTAAAAAGAGCTTGTTTTTACTGAGTGGTATGAGCAGTTTAGCAGTTTTTTTAACTGCATGTGGGGCTACCAAAATATTTGATTCCTCTGTACAGCTATTAGTGTCAGATAACTTTTCCACACTTGCTGATAAATCATTTTCACAAATGTCCTATGAAGGAATCAGGAGCTTTTTTAAAAAAAGTAAGGGTTTTGATTTACCTGAAGCAGATAGTTCACAATTACAAGAAGGCAATGGCTTGTGAAAACGTCCTGGTTTTACATTAAGTGATAGAATTGCTACTTTTAATAACATCAAAAATGATGGCTCTGATGTTATTGTTGCAACAGGTTTTAACCAACAAGAATCACTTCAAGCAATTACTTCTGATGACATTAGGTTTCAAAGTGATAAAGAAAGCCTAGCTAAAACAGGTTTTATTTTTGTTGATGGAGCTATTGAAAAGGAGTTTAATAAAAGAAATGGTGTTCCTCAATTTAAGTCAACTCCTACCAATATCTCATCAGTTGCTTTTAGAAGTGATGATGGTTCTTTTTTAACTGGAGTTGCTACTGCAGTTTACTTAAATCTTAACCAAGAATATTTCCTTGATAAAAGTGGTTGGTCAACTAATAGTAGTAATAATAACGAACTAACTGTAAGTGGTTTTGTAGGTATTACTCTACCTTCAACGCTTTCTTTTCTAAATGGTTTTCGACTAGGTATTGCTTACTTTAATGAAGTGATTTATAAACATTTAAGCGATGCACAAGATTCATCTGCACAAGTGACCACTTCTAAACAAACTGTTTTAAAACAACTTCAAGTTGCAAATGGTGAAAAAAAGATTAAAAAGATTAAATGGATTTCACCAAAACAAGGAAGTGATGGAGAAACTATAAACATTCAAGATCACCAATCAGGTTCTTTTTCAGATACTGAACCTAGAGCAATAACAATAGCTAATAATTTAATTGATAAAGGAGTTAATGCTATCATTCCTATTGCTGGACCACAAACGAATTTAGTGGTTACTCAAATTGCTAGAAGACAAGCCCATACTGCAGTTATTGGCGTTGATAGTGCACAGGAATTGCTAGATATTAATATTGATGCTCCAAATAAAGATAAGTTAAAAATGGGGAATAAAAAGATTATTCCCTTCTCTTCTATTAAGGCTTTGGATGTTGCTGTTGAAAGTATCTTATCAACATTAGAAAAAGGTTCCAGTCAAAATGGTTATCAGGGCTTTGGATATAACAACATAGGTACAGTAAAAAACAACTCTGTTGGGGTTAGTGAAGCAGGTTATGAATTTTTAATAGATCCTGTTTTTTGAAAAAATACTAGCTCAATGCAAGCTATGTCTTTATCAGCAAGTCTAAAAGCTAATGCAGCATCTTCATCAGATAATAAGAAAAAATTATCAGAAGTTGCTACTAAGAAAAATGAAAACGGTTCGACAAAAAATGGTAGTAATGGCATCATTGACAAATATGCCAAACTCTTAACAAAATCTAGTTCTTCAACTAGCATGAGAAACGGTAGTTCAGATAGCAATCAACAGAATTTTAAAACAACAGATAATGATGGTGATTGAACTATTGTTGGTGATGAATTAGGTAAATATAAGTCTAGTGAACTGCCTATTTTTACAGGTATTTCTTCATACCCAACTTTTCAAACTGAAGCACAAAATGTTTTAGATGGTGGAGCGAATGTTGCTTCAACACAAGGCTTTAAATGAAGCTTTAAACAAATTTAGAATTTTGATTACTTAAAGATATGAAAAAATTTCAAGCAGTTATTAAAGACCCAGTGGGAATTCACGCACGTCCTGCTTCTATCCTTGCAAGTGAGGCTAGTAAGTTTAAATCTGAACTTAAACTGGTAGCTCCAAGTGGTGTTGAAGGTAATATTAAATCAATCATTAACTTAATGTCTTTAGGAATTAGACATAATGACAACATTACTATCAAAGCAGATGGAGCTGATGAAGAAGAAGCTTTAGCAGCTATTAAAGCTTGTCTTGAAAAAAATAAAGTTATCTAACTTAGCATATTTTAATCAATTAAATCTGTTTATTTTTTAATAGTAAAAACACAATTTAACCTTGTTTCTATTTAACAAAATTGGTTTGAAAAAGGCTATCAATTTTGCTGTAAAAAATTAAGATATTTTTTATCAAAAATTAGCATAAAAAATTGTTATACTAATTAACGTTTTTTATTGAAAATTAAGTATTTAAATTGAACGAACATTCTTTAATTGAAATTGAAGGTTTGAACAAGACCTTTGATGATGGTTATGTTTCTATAAGAGACATTAGCCTAAATATTAAAAAAGGCGAATTTATTACTATTTTAGGCCCTTCTGGTTGTGGTAAAACTACCCTGTTGAGGTTATTAGCTGGATTTGAAGATCCTACTTATGGCAAGATCAAAGTTAATGGTATTGACATTAAAGACATGGCAATCCATAAGCGTCCTTTTGCGACAGTTTTTCAAGACTATGCTTTATTTTCCCATCTAACTGTTTATAAAAACATTGCTTATGGTCTGAAGGTAATGTGAACAAAGTTAGATGAAATTCCAAAACTTGTAAGTGATTATCAAAAGCAACTTGCTCTTAAGCATTTAAAGCTAGAAAGAAAAATAGAGCAGTTACAAAAAAACAATTCTAATGCTCAAAGAATAAAGAAATTAAAGGAAAAATTACAAAAACTTTTAGAAATTAACAAACAAAAAGTTATTGAGTTTGAAAATAAAGAAAAACTACGTAGAGAAGATATTTACAAGAATTTAGAGCAATTAACAAAAGAATGGGATCTACTTTCTCAAAAGAAACTAAAAGAAGTTGAACAACAAAAACAAGCAATTGATAAAAGTTTTGAAAAAGTAGAGAATAAATACAAAAAAGATCCTTGGTTTTTTCAACACAGTGAAATACGTTTAAAACAATATCAGAAGAAAAAAACTGAGTTGAAAGCTGATATTAAAGCAACAAAGAACAAAGAACAAATCCAAAAATTAACTAAAGAACTTCAAACCTTAAAACAAAAATACGCTAATAAAAAAGCAATTGACAAAGAGTATGACAAATTAGTTGTAGCTTACAATAAGAAAGACTATTGAACTTCTTATTGAGAAACATACACACTTCAACAAAAAGAAGCTTTTGAAAAACGCTATCTTTCAAGAAAACTAACTAAAGCTGAACAAAATAAAAAAGTTAGTGATGTTATTGAAATGGTTGGTTTAAAAGGTAAAGAAGATCGTTTGCCTGATGAATTATCAGGGGGAATGAAACAAAGAGTTGCTTTAGCACGTTCTTTAGTAGTAGAACCTGAAATTCTTTTATTAGATGAACCATTATCTGCACTTGATGCAAAGGTTAGAAAGAATTTACAAAAAGAATTACAACAGATTCATAAAAAAAGTGGATTGACTTTTATCTTAGTAACTCATGATCAAGAAGAGGCTTTAGTTTTATCAGATCGGATAGTGGTTATGAATGAGGGAAACATCTTACAAGTTGGTAATCCTGTTGATATTTATGACTCTCCTAAGACTGAATGAATTGCTAATTTCATTGGTCAAGCTAACATCTTTAAAGGTACTTATTTAGGAGAAAAAAAGATTCAGTTACAGAGTGGTGAAATCATTCAAACTGATGTTGATAATAACTATGTTGTAGGTAAGCAATATAAGATCTTAATTCGTCCTGAAGACTTTGATCTTGTTCCTGAAAATAAAGGTTTTTTTAATGTTCGTGTTATTGATAAAAACTACAAAGGATTGCTTTGAAAGATAACCACACAATTAAAAGATAACACTATTGTTGATTTGGAGAGTGTTAATGAAGTTGATGTAAATAAGACCTTTGGTGTTTTATTTGATCCTATAGATGTTCATTTAATGGAAGTTTAACAAGATGCACATTAAGAAAAAATACTGACTTCTGCTCCCCTTCTTTTTATTAATGACAATCTTCTTTATTATTCCAATGGCATGGATTATTGTTAGTGGATTACAAAGTGAAGATGGGGCTAGTATTAGTCAAAAATATGAACCACTTGTTAGTGGCTTAGGTTTTTTTAACAGTTTCTGAACCAGTTTGTGGATCTCAATAGTGACTGTAATTGTTGCATTGTTGTTTTCTTTTCCTTTTTGTTACTTTCTCTCCCAATCAAAAAACAAAATTTTTAAAGCGTTTGTTATTTCAATTGCAACAGTTCCTATTTGAAGTAGTTTTCTTATTAAGTTAATTGGATTGAAAACCCTACTTGATTTATTAATTGGACTTTCTTTAAACAGAGTTGGTGATAACAACTTAACTTTTGGTTCAGGATATACCTTACTTGGAACAATTTATCTGTTTACTCCTTTTATGTTTTTACCACTTTATAACCACTTCTGTGTTTTACCTAAAAACTTGTTGTTAGCTAGTCAAGATTTGGGTTATAACTGGATTTACAGCTTTGTGAAAGTAGTAATTCCTTTTTCTAAAACCGCAATGTTATCAGGAATTGCTTTAACTTTTTTCCCTGCTTTAACTTCAGTTGCAATTGCTCAGTTTTTAGATAACTCTAACCAAGCTGAAACCCTTGGTAACTACATATTTACCTTGGGTAATAATGGTTATGATAGTGCAATTGAAAGAGGCAGAGCTGCTGGAGCAATTATTATTGCTGCTTTAATTACTTTTGCAATTTACTTTACTGTTGTTTTTTTGCCTAAAATTGTCCGTATTGTTCATAACAAATGAAAACAACATGAAAAAGCATTTTAAGAATTTAATTAAAAACAGTTATTTCTTTCTGTTAATAACTTTAATCTATTTACCACTTTTAATAGTTGTACTTGTTAGCTTAAACGGTTCTTCTTCAAGAGGAAATATAGTGCTTGATTTTGGTAATGTTTTAAATCCTAATCCTGATTCTAAATCTGCTTATTTAAGATTAGGTGAAACTGATTTTGCAACACCACTAATAAATTCAATCATTATAGGTGTGATCACTGTTTTAGTGTCTGTTCCTATTGCTGTTATCAGTGCGTTTGCGCTTTTAAGAACAAGGAATGCTTTAAAAAAGACAATCTTTGGAATTACTAATTTTTCTTTAGCAACTCCTGATATTATTACTGCTATCTCTTTAGTGTTGTTATTTGCTAACACTTGATTAAGTTTTAACCAGCAGTTAGGTTTTTTTACCATTATTACTTCCCATATCTCTTTTTCAGTGCCTTATGCATTGATTTTGATTTACCCTAAAATTCAAAAATTGAATCCTAATTTAATTCTTGCTTCTCAAGATTTAGGCTATTCGCCTTTAAAAACTTTTTTCCATATTACTCTACCTTATCTAATGCCAAGTATTTTTTCAGCAGTACTAGTAGTATTTGCAACTAGTTTTGATGATTATGTAATTACCTCTTTAGTACAAGGATCAGTAAAAACTATAGCAACTGAACTCTATTCATTTAGAAAAGGAATTAAAGCATGGGCAATCGCCTTTGGGTCTATTCTCATATTGATTAGTGTCTTAGGAGTCTGTTTAATAACCCTGCAAAAGTATTTAAGGGAAAAAAGAAAGGAAATAATCAAAATAAGACAATGAAAAAACAGTTAAAATATTGCTTTTTCTCACTTTTTGTTAGTCTCTCATCAATATTGAGTAGTTGTGGTTCAACAACATTTGTACTAGCTAACTTTGAATCTTATATTTCGCCCTTATTGCTAGAAAGAGTACAAGAAAAACATCCCTTAACTTTCTTGACTTATCCTAGTAATGAAAAACTAATTAATGGTTTTGCTAACAACACTTATTCAGTAGCAGTAGCATCTACTTATGCAGTTAGTGAATTGATAGAAAGGGATCTATTATCACCAATAGATTGAAGTCAGTTTAATCTGAAAAAAAGTAGTAGTTCAAGTGATAAAGTAAATAATGCCAGTGATGCAAAGGATTTGTTTATTGATTCAATTAAAGAGATCAGTCAACAAACCAAAGATAGTAAAAACAATGAATTACTGCATTGAGCAGTTCCTTATTTTCTTCAAAACTTAGTGTTTGTTTATCGTGGTGAAAAAATTAGTGAACTTGAACAGGAAAATGTTTCATGAACTGATGTAATTAAAGCAATTGTGAAACACAAAGATCGCTTTAATGATAATAGGTTAGTTTTCATTGATGATGCTAGAACGATCTTTTCACTTGCTAACATCGTTAATACTAACAACAATTCAACTGATGTTAATCCAAAGGAAAATGGAATTGGTTATTTCACTAATGTCTATGAAAGCTTTCAAAGACTTGGATTAACAAAATCTAATTTAGATAGTATCTTTGTTAATTCTGATTCCAATATTGTGATCAATGAATTGGCAAGTGGTAAAAGACAAGGAGGAATTGTTTACAATGGTGATGCAGTGTATGCTGCATTGGGCGGTGATTTACGTGATGAATTGAGTGAAGAACAGATTCCTGATGGGAACAACTTTCACATTGTGCAACCCAAAATTTCCCCAGTTGCTTTAGATCTTTTGGTTATCAATAAACAACAATCTAATTTTCAAAAAGAAGCACATGAGATCATTTTTGATCTTGCTTTGGATGGTGCTGATCAAACTAAAGAACAGTTAATTAAAACTGATGAAGAATTGGGTACTGATGATGAAGACTTTTACTTAAAAGGAGCGATGCAAAACTTTAGTTATGTGAACTATGTTTCACCATTAAAAGTAATATCTGATCCAAGTACTGGAATAGTCAATTCCAAAAAGAATAATGCTGAAATGAAAAGTAAACAAATGTCAACTGATCAAATGACTAGTGAAAAAGAATTTGATTATTACACTGAAACACTTAAAGCATTATTAGAGAAAAAAGATAGTGAAGAATTAAATGAAAATGAAAAAAAACTAGTTGAAACCATTAAGAAAGCTTACACTATTGAAAAAGATAGTTCAATTCAGTGAAACCAATTGGTTGAGAAACCAATTTCTCCCTTACAACGTAGTAATTTATCGCTATCTTGATTAGACTTTAAATTACACTGGTGATAATATGGAACAGCCGTTGTGTGTTTTAGGGATTGAAACAACCTGTGATGATACAGGTCTTAGTATTGTCATTGATCAAAAAATCAAGAGTAACATTGTTATCTCTTCTGCTAACTTACATGTAAAAACAGGAGGAGTTGTACCTGAAATTGCAGCACGATGCCACGAACAAAATCTCTTTAAAGCAATAAGAGATTTAAATTTTGAGATAAGAGATTTATCTCACATTGCTTATGCATGTAATCCTGGGTTAGCAGGATGTTTACATGTGGGAGCCACTTTTGCTAGAAGCTTAAGTTTCTTATTAGACAAACCATTGTTACCCATCAACCATCTTTATGCGCATATCTTTTCTTGTTTAATTGATCAAGATTTAAATAAGCTGCAATTACCAGCATTAGGCCTTGTAATTTCAGGTGGTCATACTGCTATTTATCTAGTTAAATCATTTTATGAACTTGAACTAATTGCTGAAACTAGTGATGATGCAATTGGTGAAGTTTATGACAAGATAGGCAGAGCAATGGGCTTTGATTATCCTGCTGGTAGTAAAATTGATAGTCTTTTTAATAAAGAATTAGTTAAACCTCACTATTTCTTTAAACCTTCTACTAAGTGAACTAAGTTTTCCTATTCTGGTTTAAAATCTCAGTGTTTAAACAAGATTAAACAAATAAGTGCTAATAAAACCCGAATTGATTGGAGTGAATTAGCATCCAATTTTCAAGCTACTATTATTGATCATTACATTGATCATGTTAAAAATGCAATTAAAAAATTTGCCCCTAAAATGTTGTTAGTAGGAGGTGGAGTTAGTGCCAATTCTTATCTATCTAACAGAATTAGTACATTAAATTTACCCTTTTTAATTGCTGATAGCAAATACACCAGTGATAATGGAGCAATGATTGGTTTTTATGCATCACTTTTAATTAATGGCGATAAGAATTAAAAGTACAAGAGTTGGTAGATTTGTTTCTGAATCAGTGGGATTAGGTCATCCTGATAAAATTTGTGATCAGATTGCAGATAGTATCTTAGACCAATGTTTACTACAGAGTAAAACTAGTCATGTAGCATGTGAAGTCTTTGCTTCTAAAAACCTTATTTTAATAGGTGGTGAGATTTCAACAAGTGGCTATGTTGATGTTGTTCAAACTGCTTGAAGAATTTTAAGAAATTTAGGTTACAACGAGACTGATTTCAGTTTTTTAAGCTGTATCAACAACCAATCACTAGAAATTAATCAAGCAGTTTTAAAAAATAATGAGATTAATGCAGGAGATCAAGGCATTACTGTTGGTTATGCAGTGAATGAAACAAAGCAACTAATGCCTTTAGGAGTTTTACTAGCACACTCGTTTTTAAAACAAGCAGAAAAACTAACAAAACAATTTGATTTTTTAAAAAATGATATGAAAAGTCAAGTGGTTTTAAACTACAGTTTAAACCAAGTTGAATGTGAAGAAGTTTTACTATCAATTCAACACACTAATGCTATTAGTTTAACAGAATTGAGAAAAGTGATTGAAAATAATGTAATTCTACCTGTTTTAAACCAATATGGTTTTCAAGATAAAAAGCCAACTTGTTTAGTGAATCCTGGTGGTTCTTTTGTTTTAGGTGGACCTATGGCAGATACTGGACTAACTGGTAGAAAAATCATTGTTGACACCTATGGTCCATATGCTCACCATGGTGGTGGTAGCTTTAGTGGCAAAGATCCTAGTAAGGTGGATAGAACAGGTGCTTATTTTGCACGTTTTATCGCAAAACATATTGTAAGTTTAGGCTGGGCCAGTGAGTGTGAAGTCAGTATTAGCTGAGTCTTTTCAAAACCCAATCCACAATCTATTACTGTTAAGTGTTTTAACACTAACATACAGTATGATGAAGTGTTAATTAATAGAGTTGTAAATAACTATTTCAACTGATCGATTACTAAAATTATTGACAAGCTAAAATTACTTGATTTTGTTAAGTATTCTGATTATGCAGTTTATGGACATTTTGGTAATGATCTTTCACCATGAGAACAGCCCACTGAATTGGATAAATTAGAATGCTTAATCAAAAATTTCCATTAGATCCTAATAAAAAAAAGGAACAACAAGGCGTAGTAAAACCAAATCTACCGGTTGTTAAAGAAAAGAAAAAACAGCCATTTAAAAAACCAAATTGGAGTGAATTTAAGCTGTTCAACTTTTTTAAAAAGCACACTTATTTTTGCTTGGTTGTATTAGCTTTTCTTATCTTGATTATTTTACTTAGCAGCTTATTTGCTATTCCACTTAGCCAAATACCTTCAAGTACTGTTTAAAAGAATTGTCCAAATCCACCAAACGGGTTACTACCTGTTTTTAATAGTTTACCCATTTCTGTTGCTTTTAGTTGCATCTTATTTCATTCCTTCATCAGTTTGTTTAACTCATCCATTTTTCTCCCTGATCCTTTTATGATGCGCTGTTTTCTATTAGGATCACGATTAATTAATTTGGGATGTCTCCTTTCTTCTCTAGTCATAGAGTTAATTAAAACCTTTCATAGTTCAATTTTGTTTTCAATTAATTCAGCATTTTCTTCTGATACAGAAAAGTTAGCAGGCAACATTTTTATCAGTGAACTGACACTTCCCATTTTGTGCATTTGTTGCATGTAGATCAAAAGATCTTCTAAATCCATTTTTCCCAAAAACATCTTGCTGATGGTTTTAGTTAAATCTTTTTTATCAAAAACTTGTTCAGCTTTTTCAACTAAACTCATTACATCACCCAAACCTAAGATCCGATTGGCTATCCTTTCAGGATGAAATTGTTCCAATCCATCTAATTTTTCAGAAACACCAATTAATTTAATGGGTACTTGTAAAAGTGAAGCTAATGAAAGTGCAGCTCCTGCTCTAGCATCACTGTCTAATTTACTGATAATAAATCCAGTTAGTTTTAAACGTTTGTGGAACGTTTGAGCAACATTGATAATTTCCTGACCGCTTAATCCATCTACTACCATGATAATTTCATCAGGATTTAATTCATTTTTAACACTTACCAATTCATCCATTAATGTTTCATTTGTTTGTAATCTACCAGCGGTATCACAAATGATTGCTTGACATTTTGCAGTTTTAAAAGCACTGAGTGCTGCTTTTGTTGTTTTAGCAACTGGTTGAGTGCCTTGTGCAAAAAATACGCTGTTAGTTTGTTGTGAAAGCGTTTCAAGTTGTTCAATGGCAGCGGGTCTGTAGATGTCCAAGCCTACTAACATTGTTTTTTGCTTGTATTTCTTTTCAAGTCAATAAGCTAGTTTGCCACAAGTTGTTGTTTTACCTGATCCTTGTAAACCAACCATCATTATTTTTAAAGGTCTTTTTTCATTTAGTTCTTGGTTGGGTTGGCTTAAGATATTAATTAGTTCTGTTTTGATTGTTTTTAATAGAGACTTTTGCAAATCTTGACCAGGTTCAATGGTTTGTCCTACTGTTTTATCTCTAATTGCTTTGATGAAATTTTTAACAACAAGCAGGTTAACATCAGCATCAAGCAATGCAATTCTAATCTCTTTTAGAACTAACTCTACATCTTTCTCAGTGATCGTTTGAGCGTTAATTTTTTTTTGCATCGTGCGCATAACGATGCTTGATAACATTGCTTTGAACATGATTTTTAATTATTTATTATTAAATAATGTTTTAATAAAACAATATTGCAATATGACCCCACATATAAGTGCTAAGAAAGATGACATTAGCAAAGTTGTTTTAATGCCAGGTGATCCATTGAGAGCTAAATGGATAGCTGAGCAATTCTTAGATCAAGCTAAATTAGTCAATGAAGTGAGGGGAATGTTTGCTTATACTGGGCAGTATAAATCTAAAACAGTTACAGTAATGGGCCATGGAATGGGGATCCCTTCTATTGGAATTTATTCATATGAGTTGATGAATTTTTATGAGGTTGAAACTATCATTAGAATCGGAAGTTGTGGTGCTTTAGCACCGCAATTAAAATTAAAAGATCTTGTTATTGCTTCAAAAGCATGAAGTGAGTCTATTTATGCTAAAGACATGGGTGTTGAAATTCCAGAAGATAAGATCTTATTTGCAACAAGTTCTTTAGTGGAATTAGCAAAAGAAACTGCGATTAAGAACAAGCTTGATTTTCATGAAGGATTAGTATTTTGTGAGGATGCTTTTTATCAAACTAGAAAAGATGTAATTAGTCTTGCTAAAGAAAAAAATAGTTTAGCAGTTGAAATGGAAGCACATGCACTTTATGCTAATGCAATCCTGTTGAAGAAAAAAGCACTTACACTCTTAACAGTATCTGATTCTCTAGTAACTCATGAAGCACTTAGTTCTGAATTAAGACAAAAGTCATTTAAGCAAATGGCTTTATTAGCACTTGAAATGACTCAAAAACTAATCTAATCTATGAAATTAGAATACAACCGGATTATTGATAGCACCTTAGTCAAAGCTGATACGCTTCCCCATGAAATAGATACTTTATGTGCTGATGCTCATAAATACCAGTTTTTTGCAGTGTGTGTTAATCCTAGTTATGTTAGTTATGCTAAAAACATCTTGAAAAATACTGCAGTTCAACTCTGTTGTGTTGTTGGTTCCCCCTTAGGACAAACAACCCAAAAACAGAAGGTATATGAAGCTAAGATTGCTATTAAAGAGGGAGCGGATGAAATTGATATGGTAATGAATATTGCTGAGTTTAAAAAACGTTGTGCTTGTGTTATTACTGAAATTAGAGCTGTTAAAAAAGTGTGTGGCAAGCGTAAATTAAAAGTAATTATTGAAACTGCACTTTTAACAAATGATGAAATCAAAGATGCAGTTAATGTTTGCATTGATGGCAATGCAGATTATGTTAAAACTTCCACTGGTTTTTCTTTCCGTGGTGCATCTTTAGAAGATGTTCAGATTATGAATAATGCTGCAGCAAATTTAATTAAAATCAAAGCTTCAGGTGGGATTAAAACAGCAAAGCAATTTATAGATTTATTTCAAGCTGGAGCTAGTAGAATTGGAACTTCAAATGCGGTCCAAATAATGCAAGAATTAAACAAAATGAACCATGAATATCATTAATTTAATTAATAAAAAACAACGCGGAAAAGCTTTAAATTTAGCTGAAATCAATTGGTTTGTTAATGCTGTTTTAAACAAAACCATTGCTGATTATCAAATTACTGCATTTTTGATGGCTATTTGGTTTAAAGGGATGAACCCAAATGAACTTTTTTTATTAACAAAAGCAATGGTGGATACTGGTGAAATTATTAAGTTTAATCACCATGGCAAGATTAGTGTTGATAAACATTCAACTGGTGGTATTGGTGATAAGGTTTCTTTAGCATTGGTTCCTATCTTAACTAGTTTAGGATTTAGTGTTGCTAAATTATCAGGAAGAGGCCTTGGTTATACTGGTGGAACAATTGATAAATTAGAAGCAGTTGGAGTTAAAACAGAATTAACTGACCAACAAGCACAAGCATGTTTAGATAAAAATGATTGTTTTATCATCGGACAAAGTAAGGACATCGCACCAGTTGATAAAGTACTTTATGGTTTAAGAGATATTACTGGAACAGTTGATAGTTTGCCTTTAATTGCATCTAGTATTATGTCTAAAAAGCTAGCAGTTATGAACGAGTATATTTTCATTGATCTTAAATATGGAAAAGGTGCCTTTTGTAAAACTAAGAAAATTGCTAACGAACTTGCAAAACTGATGCAAAGTATTGCTAAAAGTTTTAAAAGAAAGCTGTCTGTTAAATTAAGTGATATGAATCAAGTACTTGGTAAAGCTGTTGGCAATGTAATTGAAGTTAATGAAGCTGTTAACTTTCTAAAACAAGATTTAGATCAAGTAGGACAAGATTTTATTGATTTAATGCAAACAATTGTTATTAACATTCTACTTGAAACAAAACAAGCAAAAACCAAACAAAAGGCTATTGAACTTTATCAGGATGTTTTAACTAGTAAAAAAGCATGAAATCGCTTTTTATCTTTTATTGAATCTCAAGGAGGAAATGTTGAATTATTTACTCAAAAAGAAGGTTTTTTTAAACCTAAGTATAAGGCATCTATAAAAGCTGAAAAAAGTGGTATACTACATTTTACTGATCCAATTGATTTAGCTAAAATTGGGATTAATCTAGGGGCAGGTAGGATGAAGAAAACAGATCAAATTGATCCAATGGCAGGGTTATTTTTAATGAAAAAAGATAATGAGTCTGTGGCAGTTGGAGACACTGTATTAAACCTGTATAGTTCTAGTCCTATTAGCAATGAAGATATCTCTGCTGCTCAAAAAACAATAATTATTAATAAATAAAAATTCCTATGAAGGTGAATTTAGAGTGGATAATTAAACAGTTACAAATGATAGTTAAAAGAGCATATACTCCCTTTTCTAACTTTAAAGTTGCATGTATGATTATTGCTAACAACCAAACTTTTTTTGGAGTTAACATTGAAAATTCTTCCTTTCCAGTAACTTTGTGTGCTGAAAGAAGCGCCATTGCTAGCATGGTTACAAGTGGTCATAGGAAAATTGATTATGTTTTTGTTTACTTCAATACTAAAAATAAGAGTAACTCACCCTGTGGAATGTGCAGACAAAACTTACTGGAATTTTCCCATCAAAAAACAAAGCTTTTTTGTATTGATAATGATAGTAGTTATAAACAATTTTCCATTGATGAATTATTAATGAATGGTTTTAAAAAGAGCTAATGGATAAACTTAGATTAGAAGTTGAAAGATGGTTAAATCATCCTAATGTTAATTGGGAGTTAAAACAACAAATTAAGGAGTTGAATGAATCAGAAATTCAAGAACTTTTTAGTTTGGAAAAACCTTTATTTGGCACTGCAGGTGTAAGAAACAAAATGGCACCAGGTTATCATGGTATGAATGTTTTTTCTTATGCCTATTTGACCCAAGGTTATGTTAAGTACATTGAATCAATCAATGAACCAAAGCTTCAACTACGGTTTTTAGTAGCACGTGATACAAGAAAAAATGGTGGTTTATTTTTAGAAACGGTTTGTGATGTAATTACATCTATGGGTCATTTGGCTTATGTGTTTGATGATAACCAGCCAGTTTCAACACCTCTAGTTTCCCATGTCATTTTTAAATATGGTTTTAGTGGAGGTATTAATATCACAGCTAGCCATAACCCTAAAGATGATAATGGTTTTAAGGTTTATGATCATACTGGTGCACAGCTTTTAGACACACAAACAAACCAATTGTTAAGTGATTTACCTTGTGTTACATCTATGCTAGATTTGGAATTACAACCAAATCCAAAGCTTGTCCATACTCTTGACAATGAAAAGGTTTATAAAAACTATTTCAGAGAGTTGAAAAAGGTGTTGGTTATTAACAACAACAATTTCAAAGACATTAAGGTAGTTTTTAGTGGGCTTAATGGGACTTCAGTTTGCTTAATGCAACGCTTTTTAAAGTACCTTGGTTATAGCAATATTATCAGTGTTGAGGAACAAAATTGGTTTGATGAGAATTTTGAAAATGCTCCTAACTTAAATCCAGAGTATAAAGATACATGGATATTAGCACAAAAATATGCTAAGAAAAATAATGCTAAGTTAATTATTATGGCAGACCCTGATGCTGATAGATTTGCAATTGCAGAGTTAAATAATAATCAATGACATTATTTTTCAGGTAATGAAACAGGAGCAATTACTGCTTACTATAAACTTAATCATAAGGTTTTTAAATCACCTTACATTGTCTCAACTTTTGTCTCAACTTATTTGGTAAATAAGATTGCTAAAAGATATGGCGCTTTTGTGCATAGAACCAATGTTGGTTTTAAGTACATTGGTCAAGCAATTAATGAGTTATCACAAACAAACGAATTAGTTGTTGGTTTTGAAGAGGCAATTGGTTTAATAACTAGTGATAAATTAAACCGCGAGAAAGATGCTTATCAAGCTGCTGCATTATTGCTTGAGATTGCTAGACATTGCAAAGAACAAAACATCACGCTTTTAGATTTTTATAAAAGAATTCTTTCTGAGTTTGGTGAATATTTCAATTTAACAATATCTCATCCCTTTAAAGCTACTGCTACTGATTGAAAAGAAGAGATTAAAGCTTTATTTAATCAACTTATAAATGCTAATTTAACTGAAGTGGCTGGTTTTAAAGTAGTTAAAGTCCATCTTGATAAACAAACAAATATCTTAGAGTTTGGTTTTGAAAATGGCTGGGTTAAATTCCGCTTTTCAGGTACTGAACCTAAATTGAAATTTTACTTTGACCTAACTAATGGCACTAGAGAGGCTCTAGAAAAGCAAGCTAAGAAAATTTATAAATTCTTTGTAAATTTACTCAAACTCAACAAAGCTTAAATTTTCTAAAGGTAAGTTAATTAATTGGGTTTTAGATGTTAATTCTATCTCTACAACTGCTTTGTTAACTGTACCTTGATAAAGTGCTTTTATCTGTCCTTGAACATGAATAGTATCAGTTAAATGTACCTCTACTAAATTACCAACACTAAATTCAAAGAGTTGTTCAACTTTATTGTCAGTATCAAGAATTTCATATTTGCCCAAAAAAGGAACTAATACTGATGGATCAAGTTTGGTTTGCTCACTTTGAATAATCGTTGCTTTTTCTTTCCATTCATCTTGTTCTTGACTATTTTGTAGTTTTTTAAAAGCTTCTTTTAGTTTTTCAGCATCAACAATTTCTGAATCTTCATCCTCTTTTGGTTTTTGAATGGCTTGTTTTTGTTTTAAGATATATTGAAATTTTTCATCAAATTTATCACTGTCCATTTCAATAATGGCAGTATTTGTTAGCATAATTCGTTTGCTAGGATTGATTGATATCCCTTTAAGCATTTGTTCTAAATTATTAGCATCTTTTTCATCTAAAGGGATAGGTAAAGCACCCCTGCCAGAAGAACCTACTATGCCAGTGATCCCAGGGAAGTTACGAACAATTCTTCAAGCATCTTCACTATAAATCATCTTTATGTAGATGTATCTACCAAGGAGATTTTTTTCACTAATTTTTACCCTAAGATAACGGTAATCATCAAGAACAAACCATTTGTTAAAAGTAGTGTTCTTTAAGGAACGAGGAAGTTTTCCTGATTTTAATGAATAAACTTCTTCATGAACTTCCCTTTCTTTTAGAACTTTAACATCAACAATCTCATGATTAAATCCTAAAGCTTGAATTTTAGCTTTTAGATTTTTTACAACAGCTTCATCTTTAATACTAACAGGAGCTACATACCACTTTGGTGTTAATTCACTAGCTTGCATAAATTAATTTTTAAATATTCCCAACGTAATTAACAATTGATTGATACCAAAAAAGATTCCTACCATCAGTCCACTAACTAGCAAAATTAAAAGAAAAATTATGATAAGTTGTCTACCTTTTACTCAAACAATTTTGTTGATCTCTTTATCAACTCCAAATCATAAATTAACAATTCGTTTTTTAAAAGGAAGCTTGGGTTTCTTTTCCTTTGCTAAACTTTCATGCTTTTCTTTTTCTTTAGTTCTTTCCTTATCTTTACTTTTCTTGGCTTCTGTTCTAAGTTTGAGCTGTTTATGTAACTCATGAATTGAAGCATCATCATAAGCAGTTAGCTTTTCCTTCTTTTTAAAGCTAAAAGGCAGTTTTTTTTCCATGGCTTTTTAAAAAGAATCTAGATGCTTAGTTTTTTGATTGCACTGCTTGCAGTACTTGTTGATGATAAGTCGCTGCAAAGGTTGTTTTGTTCACCGTTTTACATAGTTACGACTTAAACAGTCTTGACAGACAAAAATAATCTTTTTGCGCAACTTCAGTAGCAAATTATAAATTATCTGCTTGTTTTACTAATTTAATCTTTTGATAGCAATAGAGCTGTAAAAGGCCAAAGGGTAAAAAACAAGATCAGTGTAGAATTTTTTTGAATTTAGGTTCAATATTTTCACATGCAGTATTAATTGTTTTTCAAATCATTATGCTGATAACTAACTGGATGATCATAAAACAGATAGCAAAAACTCCTAAAACAATTAAAACTGCTCGTAAATTAAAAAAAAATAGGATCACTTCTAATTGCACTTAAACTAGCTTTTCTAATTAAATCAAAGATGATACCAATCCCTACTAAAGAGATAATACAAACTAAAAAAAAGATACTAATTAACAAAAGAATCTCATTAAGGTTATGGAGTTTTTTAAATACTTTCATGGAAAAGTGTATAGAGCATACTTGATTTTAAATGCATTTTTTTAGCTAAATAATTGCATGCATCTTTTAACTTAACACCAATATCCATTAACTTCTTTATTTCATATACTAAGTATTGATTGGAAGATAATGTTTGGTGATTAATATTTTGATTATCAATAACAATTACAAATTCTCCTTTTAAAGTGATGTCAGGTAAAGTGTTTTCACTGGTGTTAAATCAATAATGTGATTCATGTAACTTAGTTAATTCTCTTCCTATAAAAACGTCATTGTTTTTAAAAACATTTTTCACAGTTTCTAAAGTATTTTCTAACCTATGCACTGCTTCAAAAAAAACGATAGTGCTTTTCTGATTTTGATAGGTACTTAAATAATTTTTGAGCTGATTTTGTTTGTGACTTAAAAAACCTAAAAACAAAAGTGGTGTTGTTTTAAAACCACTAGTGATCAATCCACACATTAATGCACTAGGACCATTAATAACTTCGATTTTTATCTCCTTATTTTTTGATATGATCCAATTAATCATTTCATTACCAGGATCAGATAAACTAGGATAACCTGCATCACTAACAAGACAACACTTAAAGTTAGTTATAAATTCCTCAGCAAAAGTTAAATTCTGTTTTTCTTTAAAACTGTTGTTAATAACAAACTTTTTTTGCTTGCAATCAATGTTGAGTAAATCCAGCATTTTTCTTGTTACTCTACTATCTTCACAGAATAACACTTCACAATCTTGTAACGCTTTTTTAGCTCTTTCACTTATCTCTTGAATATTACCAATTGGTGTAGCAACTACTTTAAGTGTTTTCATACATCTTGAAAAGTTAGATTTAATAAGTTAAGTTTTTTAAATAGTTGCTTGTGGTTAAAATAAGGTAAATTAAGATTATTGCATAAAAGTAATCGCTTTTTCTTATCATTTAACTTTAAGCTTAAATACTGATCTCATAATATGCTTTGATTAGCATTTTTTGTGATTGCAACACGATTATTTAAAGCAGCAATTAAAGCACTAGTTTCCATTTCAGCAATACCAGCTTTTTTAGCGTTTTTTAACCTTGCATTCATATCAACATAACAGTTGTAATAGTTTGTTAGATGTTGTTCTAATTTACTTCTAATCTTTTTGCCTTGTTGATCTGGATCTAATAACAAGATAACAGTTTGCTTTTCACTGATTTTTTTAATTAGGTTAATAGTTTCTTTTTTTAAAGCTGAACCATTTGTTGTAATTACATCTACATCAAAAATTTGCTGTAATTTAGCTTGGTCAGTTTTACCCTCACAAACAATTACCCCATCAATTTTTATACGTGCTTTTTGATCCATTCAATGTGACGTTCATATATTGCAGAAACTGATAAGCTGTTAGCGTAACACAGAACAACCTCTTCATATAAAGATGCTAGATCAACTACTTCAAACTGCTTTACAGCTTTAAACTTATATTGAGGAATAGAGTTTGATACAAACAAGAAATCAATTAGTTTTTGATCAAATGCTTCCATAAACTTTTGTTCTGCATCATTGTTAAACAAACCATGAGTTGCCATTACACACACTTTTTTAGCTTGTTCTTTTTGTAATAGCTTGGCTGCTGCAATTACTGTACCACCAGTATCTATCATGTCATCAACTATTAAACAGTTTTTATTTTTCACTTCACCTAAAACATTAATTGATTCAGCAACATTATGAGATGGTCTTCTTTTATCAATAATGGCTAATGGTAGTTCTAGTGTATTTGCAATTAACCTTGCTCTTTTAACCCCACCATAATCAGGGGAAACAACCACCAAGTCTTTTTTACCAAGTAGTTCTATAACTCTAAAAAGAAAGATGTGATAAGTTCTTAAAGAATCAACGGGAATATCAAAAAAACCTTGGGTTTGATCACTATGAATGTCAGTTAGAACAACCCTGTTAGCACCTGCTTTTGTTAACATATCAGCAATCAATTTACTGGTAATTGGTTCTCTTCCTTTTGTTTTTCTATCTTGTCTTGCATATCCATAATAGGGTAGAATGGCAGTAATACTTTTAGCACTACCTCTTTTCAATGCATCAATAGCAATTAAAAGTTCCATTAAGCTATCGTTAACATTAGGACAGGTTGATTGAAAAATATAGATATCTTTGTTACGAACTGATTCATCAAAACGGATATAAGTTTCACCATCAGCAAAGTGTTCGCAAACCATTTTGCCCTCTGAAATATTTAGTTTTTTGCAGATATTTTCAACCAGTGTTTTGCTTTTAGATAAACTAAAAATAACGTGCTTTTTATTATCGATACTCAGCTTCAAGCTCGCGTCGGATTGTTTTCTTTTTAATAGTTTCACGTTTGTCAAATTTCTTTTTAGGTTTTGCCAATCAAATTTCCACTTTAATTTTGCCATTTCTAAAGAATACTTTACTTGGGATTACAGATAAAGATTGTTGTTGTTTTTTATTGATAATTTGTTTAATTTCATGTTTATTTAATAAAAGTTTCTTAATTCTATCTGAAGCGTGATTTAATGGACCTGCAAAACTATAAGGTGGAATAGTGAACTGTTCTAAAAAAAGCTCATTGTTTTTAACAAAAACATAAGCTTCTTTTAAACTACCTTGACCTAAACTTAAAGCTTTAACTTCACTTCCTTTTAAAACTATTCCAGCACAATAAGATTCCATTAAATGATAGTCATATTTAGCCTTAGGATTGTTAACAAGAATTAACATTTATCAATGAAATTATCTGTAATTATACCTACTTACAATTGTGCATCATTTATTGAAAAAACAATTAATTCAATTGTTAAAAATAGACCTAATGATTTGGAAATAGAAGTTTTAATTATTGATGATGGATCAATTGACAATACTAACAAAGTTATTAAGAAAATTCAAGACCAAATTAATAATTTAACTTTGCAGTATTTTTACAAAAGTAATGGTAACTGGGGTAGTGTTATTAATTATGTTAGAAACAATAAACTAGCAAAAGGGGAATGAGTAACAGTATTGGATAGTGATGACATTTTTTCAAAAAAAACAATTTCTATTTTTCAAAAATATGCCCAAAAACAAAGATATGATGCGATTATTTTTGACTACTATAAATGCTGAAAAAAGTTTTTGTGAAAAATTCCTACCTATGCAAGGTTTAGAAAAGAAATTAAAGGTGAATTGAAAAAACAAACACCTTTTTGTATTCCCTTAGCTAAGTTTTTTAAAAATGAGGTTTTCTATCAACTTCCTAAACTAAGAGAAAATGTTGGTTTTCAAGACGCTATTTATACGATGCATGCATTACAAATTGCAAATAATGTTTTCCATGTTTCTAAAGCTGGAGGATATTACTTTTTTAAAAGGGTAGGTAACTCTATGAGTATCCCTTGACACAGTTCTAGGTTTGATATTGAAGTACAAATCTGCAAGGATCTGATTGAAAATAATGCGCAAGAGATCGCTTTAGTGCATTTACTTCGTTTAAAATTTCGTAATTTAGTTGATGATAAAAAGATTAAATTTACAGTTAAAAGAGACTTTTGTTTTAGTGGTTTTAGTTGGTATAGTAGGTTAATTTTATCTCTGATGTATAACTTCTGATTGAAACGTTATTTCAACAGTTCTGAATAACGATTTTTTTGCTTATTTGCAATTACAAACTGTCATTCATTGAGTTTTCATTTTCTTGTCATTTCCTGAACCAATTGATCACCATCTTTAATCCAACAAGCAGGAAAGAACTTAACAATTAGGTTTGCTAGTGCAATAAGTCCTAAGAAGAAAACAATACTTACAATTACCCCTGGTAAAATACTTGTACCTTTTCCTTGAATTAAAACAGGCGCTTGGAGAAATACTGATTGGGTAATGTCAAATAAGGTGTAAGCAATATAACCAAAGCCCCAACAAAATCCAAAAAGAATAGGAAGTTCATTCTTCTTGTAATCTTTAAATTCATATGGCAGGATTAAATTTGAACTTGATAAACTCCAAGCAAATGCACCACCGATAAATGAAAAGATACTAATTAAAGCAAACCCAGCTGCACTACCAATACCAAGTGTTGCTGCAAACATGATGATTATAAGCAATACCACTATGTTAGCTGTTAATAAGAAGTGCATTCACCTCTTCTTGTCATAAATGGTTTTATTAAATGGTGAAAAAACAACAAAGCCCAAAGCATAACCAATAACTCAAAAAATAGCAAGTGTTGCTAAACCTGTTGTGTACACACCAGTACTTATCAAACCATTTGATCCTGATGGGGAAACAGTTTGCAAGATGCTAAATCAAGCTGGAGTTAACGGATTGACTATTAGTATTAAAACTATGCCATAAACACCAATTAATTTTCATGTTGTTTTATTCTTTAAGATATCTATGGATTTTGGTTGAGTAGTTAAACTCATATTTTCCTTAGTTTGCTTTTGAGGAAAGATGTGATCTATCTTGCTTTCAAACCATAAAAATAAACATAAGTTAGCAAACACAACCAAAATCATCACAGTCATAATGTAAACCCAGTATTGAGATGCTACTTGTTGAACTTGTTCAAATAAAAATGGTGTGAAAACTACTGCAATCCCAATGTTAAAACCCCAAAGGTTAGCATTAGATAGGATTGATTTTTTTCTGTTTGAAGAAAGATTAGCAATTGCTGGTTGAGTATAAACTACTAAGGTAGTTCCACCAATAGCAATTGTGCTTCTAAAGATAATAAACAATGCATAACCAGTAATTGATGCAGCTGTACCAATACTATTACCATCCAAACCCTTAATAAGTTCAACACTTGTTCCATCTGCAAGCATCGTTTTACCATTGATCATCACCGGTCCAAGTAATTGACCCACTTGTAAATTGCTACTTAGTGAACTTAATTGAGTTTTTACTGAATCTGATAAAGGTCTTAATAAAGTTAATTGATTATGACCATTCAAAGGGTCTCCTATAATAAGAAAGGGAAAACAAACACACATGATTCCCATCATGATTAAAACCGCATAACGATAACCAAATTTCAAAACAACAACTCCACAAAGAATAGAACCTACTGCTCTCAATAAAGTGATAGTTCAGTTGGTGGAAGATGTGGCTATTTGCCCAGCATTTTCAGTAAAGAAAGAACTTAACCAACCACTGTACTGTGGTAAAGTGGTGGTTGATTGAGTAAGTATACCAGTTGGTTTACCACTAATTCGATCAATTACAAACCATTCAACTACAAACAATAGATACCCAAAAATTACTATGATCCACAGGGTAATAAGTTTCAGATCACTTACTTTTTTTTGACTTTTATTTTCCACAAATTAAGACAGAATTTACATTAGACTAATTTTAAAAGCTGTAGCTAATTGTTTTTTAGAACAAACTGAATCAAAGAATTTGAAATTGTCTTTCATTGTTAACAGCATTTAACAACAAAATTGAATAAAAATAAAAAACAGACCCTGATGGTCTGTTGGATATTTAATGGCGGAAGCGGTGGGATTCGAACCCACGCACCGTAGAACGATCTAACACCTTAGCAGGGTGTCCTCTTAACCACTTGAGTACGCTCCCAGTTTGTTGTTAAATTTATTATAAATATGCAACTAATTAGCGCTTTAGATGTTTAAAAATTTATTAAGACCATCCCTATTTTTTAACTGGAGCCAAAAAACATTTAAAAATAAATTTTCGTTTTTAAAACAAGCAGCAAATGCATTACAAAAACAGGCTGTTATCAATGATAACAATGTTGCATTTGAAGCTTTAAAAAAGCGTGAAGAAGAGATTACAACTGGAATTATTACTAGTTTAGCTTTACCTCACTTACAAAGTCAAAGTGTTATAGAACCTTTTGTTGCTGTATTTAAGGTTAAAAACTTAGATTGACAATCATTAGATCAAAAACCAGTTAAATTGATATTTTTAATTGGTGTTCCTGTTGACAAAACCAATTTGCATCTTGATTTCATTAGTAACTTTTCCAAGTTAATGTTGAATGAAACATTTGCAAGTAAGGTTTTAAATGTCACTAGCTATAACGGCTTGATTAAACTAATTGATCTTTTTAACCAACAAAAAGTGCAAGACCAACCAGCTGTTGAAACAAAAAAAGAGTATGACTTTGTTGCTGTAACAGCATGCCCAACTGGCATTGCCCATACTTTCATGGCTAAAGAAGCGTTGGAAGCATTTGCAAAAAAGCATAACTTATATGTAAAAGTTGAAACTCAAGGTACAGATGGGATACAAAATCAACTTACAAGTGACGATATTAATAACGCTAAAGGTGTTATTCTTGCTTGTGATCGTTTAATTGATTTCTCTAGGTTTTATGCAAATAAGAATGTGATTGAAGTGTCAACTACTAAAGCCATTAAAAAACCTGATGAGGTGTATGAATTAATTAAAAACCAAAAGGGTAAACAGCTTGCAAATAGTGCTAAACCAACTAATCAAACCCAATTAGCTGAAAGTGAAGGGGAATTCAACTTTAATAATTTTCACAAGCGGATTTATCGTGCCATTTTAAGTGGTGTTTCTTACATGCTACCATTCGTTGTTTTTGGTGGGATATTAATTGCACTCTCTTTTCTAATTGATATAAATAACGCTAATAATGCTGGTGGTAACTTTGGCACAATTAATCCTGTTGCTAACTGGTTAAATAAGCTAGGAGGAATTTCCTTTAGTTTAATTGTTCCTATCTTATCTGCATACATTGCATATGCATTGGTATCAAGACAGGGATTATTACCTGGTTTTGTTGTTGGTTTAATCTCTTCAGGACAATTTTTATTAAATATTGTTCTGACCAATGGAACAATTGAATGGTTAGCACCATCACAAGTATCAAGTGGCTTTTTTGGTGCTATCTTTGGAGGACTTTTGAGTGCTTGCTTAATAATTGTTCAACAAAACTACATCTATAAAAAATTACCTCAATCTTTGCAGGGAATTAAAAACATCTTGTTTATTCCCTTGTTTGGTACTTTATTCACTGCTGGTTTATTTTGGGTTATTAACATTCCTTTAATTTATCTAAACTATGGATTAAGTCTGTTTTTAAATATTATGAACAGCCCTATCCTAGCACCTTTACTTGGTTTTGTAATTGGGTTGATGATGTGCTTTGATTTAGGGGGGCCAATTAATAAAGCAGCTTATGTTTTTGGTGTTGTTTCTTTACAAAATCAAAATGCAGGAACAATTGCGATGGCTGCAGCTATGCTATCAGGGATGGTACCTCCTTTATCAATTGCTTTGGCAGCTTCCATTCGAAAGAGCTGCTTTGATAAACAGGAATTACCTGCAGCTTATGCTTGTTATCTGATGGGATTGAGTTTTATTAGTGAAGGTGCTATCCCATTTGTTGTTAAAAAACCTAAGGTGATGTTAACTGCTAACTTAATTGCTGGAGCAATTTGTGGAGCATTAACAGGAGCATTTGCCTTATCAATTCGTGCTCCTCATGGCGGTGTTTTTGTGTTTGCACTTTTAAAAACTACTTTACAAGGGATTGAAGGAGCTACATTACAAACTGGAGTTGGCATTGGTTTGGCATTGGTTTGTTTAATAATTAGTATGATAGTTGGTAGTAGTATTATCATTGGCTATGACTTGATTGCAAAACATAACCAAAGAAAGCAAAATCTGAATAGTTAATTACGCTTGCGCAATTGATTATTATGTTGATTTAAACAAGCAAAAAAATAGTGTTTTAATACCTGGTGGTAAGGGGATTAATGTTGCTATTGTAATGAAATCACTTGGTTTTGATCCAACTGTCATTACTTTTTTGGGACAACCCACTAAAAACTTATTTTTAGAGTTGGTAAAACCTTATGATCTAAATATAGTTAGCTTCATTTCTGAAACTAAAACAAGAATTAACCTTAAGTTATTAAAAGATGAAAAAACTACTGAAATTAATGATTTAAGTCCTTTAATAACAGATGCTAATCTAACTGAATTGTTAACTTTTTTAAAAGCTAATGTTAAGAATAATGATTTGGTTATCATCAACGGAAGATTTAAATTTGAAGCTTTAGAAAAAGTTCTAAACTTGGTCTTTACATTAACAGAAAATGTGGTTATAGATGTTGATGAAAGCAAAATGTTAACGCTTTTAAATCAGTCTAAACCACTAGTTATGAAACCTAACATTGATGAGTTTCAAACTATGATTAATACTTTTTTTCACGATCAACAAAGCTTAATAGCAGCAATTAAAAAATTTCATTACTGTAAGCTCTTATTATTATCTGATGGTGACAAAGGAGCTTATCTTTTTGATCAGAATAAGTTATTGTTTGTAAGTTCTATCACTCCTAAACAAGTAGTTAGCACCACAGGAGCAGGTGATACTTTGTTGGCAGTTTTTTTAGCAAATTTAATTCTAAAGGTAGATTTAAAAACTGCTTTGATTAAAGCAACTAACTATGCAAGTGCAACAATTAGTAAGTTAGGTGTTGTTGATAGTAAAGACAAAATTAGTGTTATAACCCCAAAAAGTTACTATTTATAATTAATATTTGATGGTAAAAAGAAAGCGAAAGCCTAAGCTTAATTCGCGTAATATTTTAACTATCCAGATTGTTTTAGCAATCTTTAGTATGATCTTTTTTCTTACTTTGCTATCTTTAATCTTGTTTTTGAGTTTGCAAAGTAATTTAGCTACAGCTTTAGTTGAAAACAGAAATAAAGCTGTGGAACTTGTAGATAACATTGTCTTTTTTTAAAAGAACTGATTTTTAAATTTGTCTCTGACTAATTTATTAGTGTGTTAAACCTAATTAAAAATGTTATTCGTTCTTTAAAGAGTGCTAAGATTGCTTTAATAGCGTTAACTTTTTTAATTTTTGTTGCTGTTGGTGGTTTTGTGTTGTTAAATAACACAGTTAATAATTTTAACGCCGCTTTTAACTATGTTACCCACACTGGTAAATTAAGTAATGCCATCATTAATGAACGTTATGACTTTGGTAAATTAGAGTTTCAAGAACAGACCAATAATTCTCAGAATAGTAGCGACAGTTTTACTTTAACTTTAACTAACGATTCAAGAACAAGTTTTATTAATAATGCTTTGAGAACTAACCCTTCTTTGTATGAAGGATTAGTAACCCAAACTTTTAGTTATCAAAACAAAACTGAAATGACTGAAAAAACCAATATAGTTAATCAGTCTAAAATTATTGCTGCTAACAATCTTAACAATGCATTAAGTAAAGATAAACAGCTCTTAGTTTCAGGTCAACTTGAAAAACTAAATGCTGTTTTTCGAGAATATAAAGCTATTAATATTACTGACAAAAGTGTTTTTAAAAAATTGATAGTATCAGAACCTAATGATTTGGTAAATAGCCTAGTTATTTTTGATGGTCAAAATTTATCTCTCTCCAAACAAAGTTATTTCAATAATTTTTTAAATCAATTTAACGAAATTAAATCAAAGGGTAAAGATAATTTAAGTACTACTTTAAAAACTGGGCAGTATCAGGCATTTTTACAAACTCTTTTTGATTATGTTCAAGCGAGTGAAACAACATTAAAAGATCAGCTTCAAAAGTTAATTTCAAATCCAGATTCAAGTAAAACAAATCAGGTTAAAAATCTCTTTGATACTCCAAGTACGCTTACTAATATTGGGGGTCAATTAACCTTACAATGAACAGAAAATAGCCTAACAAAACAGATAGTTATCTTTGATCCTAGTAGTTATGAAACAATAGTCGCTCCTGGTAACTGAACTTATCAACAACAATTAGGTAAAGAAGTTTATCCTGATATTAACAACTGAGAAAGTATTAAAAAACTACCACTTGAACAATTTGAAAGTGAATTTTTAAAAATTGATCAAAAGTATAAGATCAGTATTGATAATATCGATTATTTAGTTATTGGGGTTGGAATTAGTCCAGATTTTGTTTATCCTGTTTTTAGTGCATCTTTAATTGTTCCTAACATTGAAAATGAACAACTTTACTATGTTAACCAAACTGGATATGAAAGAACTTTTTCCTCTTTTTTAACAAATCCAGTTGAAACAGCAATAGTAGCAAGATTGATTAATCTTGAAAGTGATCTTAATACTATCAACCAGTGAGCTGTTGAAAACATGTCATGACCAACAAACATTAAAGCTGCATACAGTAGTTCTGATACCACTAATATTTTGAATTTACTAGCAGCAAGAACAGTTTTTATCCCTAATCTAATTAACACAATTAATTTAGTGGCTTTGTTTTTAACTATTGCTATCCTAACTGTTGCTATAATTGTCAGCATTTTAATCCTGATTAGTTATTTAAAGAAAAATACTGAGCAAATTGGCATTTTAAAAGCAAATGGGGTAAGTGGTAAAAAGATTAACCTTAGCTTGTTAATCTTTGGGTTAATTCCTGCTATAGTAGGTGCTATTTCTGGATATAGCTTTGGAATTGGATTTCAAGACGTAGCTATTCATCTATTTAGTAACTATTGATTTATACCAACAGCAACATCAAGTTTTTCAGTAGTAGGATTGTTGTTTTTTTCACTGTTTGTTATCTTAATTATGAGTAGTATATCGCTTTTAGTGGGATCAATTATCTTAAAGAAGGATGTTGTAAAGATTTTAAAGCATGACAGTGAATTTAAAGTTTCAAGATTAGGACTTAGTTCTAAGAAATTGTTTGCTAGGTTTGGTATTATGACCAGGTTTAGAGTAGCATTAGCATTTAACGCTCCTTGAAAATTAGTTTTTCTAACCTTGATGAGTTCATTTACAATGATGATTTTAAACCTTAGTTTTGCAACTAAAGATAGCTTTGAAAATGCTCAATCAAAAACTAATTTAACTAATCAGAACCACCAATATGAATTTGAACTCGCTTCAGCAACAACACAAAGTGGTTTATTGAAGTGACAGTTATTTGCAGAACTAGGTACAACTGATAAAAGAAGTGAAAGTAGTGTAAAGCTTGCAAATAAAAGGATGGATATTAGTAATGTTGATGCATCTAAAGATTGAAAGAACCAACAAGTAATTAATTTTTTAAGCGATGCTAGTGGCTTTAGTAATGATTTAAATTACCTTGAAAACATTGTTCAATCCAAGATAGGTTTGGATTATTCATTGGGATTTAACAATATTGTTTCAAATCCCTGAAGGTTAAGTGAAACATTAATGCCAACTAACCAAGCATCTGCTTCCAACACTGCTTTTCAAAATTTTTTAAAAGCAATCATTACTATAAATCCAAGCCAAGGATCGCAATTCATTAAACAAACCCAAGATCCATTAACAAAAAGATTTATCTATGCAATTGACAGTGATAAGGCATTAAAAAATAATAATGAACAAAACGGTTCCCAAAACCACTTAACTTTAAATGATGATTTTGCTAAATTTCTCTACAGTCAATTTGAATTAATTAAAAAGAGCGGGAATGCAAGTAATGAAGATTTAAATGCAATTGATTTTGAAAACCCCCAAACAATCAGAGATTTTTACAACAAGTACAATGCTTTACCACCATTAGATTACAAACTTAGTTTTAATGTAATAGGTTTACCCAAAGAGACAATTGCTGGACAAATTGACACCCCTAAGTATGGATTTTTAACCCTTTATGGTGAATATCAAAATACTCCTATCAAGATTAAAGGTATTAAAGATTGAAAAGATAAAGTGGATAATTTAGGTCCAGTTTTGAGTGATCAAAACAACCACATTATTAATCAAGAATTGTTTAAAAATTATTCTTTTAATCCTTTGATAGTTAACAATTCTGCTGCAAAAAAATACCAACTTGCAATAGATAGTGAGATTAATATTGCAGTTAACAACAGCTTCAAACGGATTGACAATAAGATCATTAATCAAGATCCTTTAGTGAATGCTACCTTTAGAGTTGTAGGGATTAACAATTCCGCTCATGATCCTGAATTTTTCACTAGTTATAGTACTGCTTTTAAAGTATTGGAATATCCCAATGAATGGTTCGTAAAAAAACTTCCATTTAATAGCTTCTATGCTAATTCGCTTTTAAGTTTTGTTCAATCTACTTCGCTATTTTCTGAATCTGGTATTTTTCCTGCTACTAGTAGTTTTTCAACTAATAACACTGTACTTGTTGAGTTAATTAAAAAAACCATTAATTACAAGAATGGTCAAATGAATCAAACTTCAAGTAATGACTCTTCTAAGAAAGAAAATTACCAAAAATTGCAAAAAGCATTAGGAATATCAACTGATTTGGAGATTAGTAAAGTTAATGAATATGTTGCTATCTTAGCAAGGGTTTATAATGGTTTACCTTACAACTCTACTATTAGCTTTATTAGCAATGTTGCTGCTAACAACGCTTTATTTGGAAATATTGCTAACACCACCAAGCAGATTCAAGCTGTTGTAATTGCAGTGATTATTCCTATAATCATGTTGATTATTCTTTTGGTTTCAACTACCTTAATTCAAGAGTTGAAAAAAATTGCTATTAGATTAAAAGCATTGGGATATTCCAATTTAAAAATTCTCGCTTCATTTTTATCAATATACATCCCTTTATTTGCCTTTGGTTTGTTGATTTCTATCCCCTTTTCTATCTATCTAATTGCACTACATAATGAGGTAATTTTTGCAAGCTCATCGATCTTTTTAGATGCTTTTTTAAGTTTTGAAAGTGCAATTGGTTCAATGTTAGTTTTACTAGGGGTTTTATCAATTACCTTTATGTTGAATTGATTAGAGTTGAACAAAATTAAGATTGACAAGGAAATCAAAAACTCCTAATGGATTTTTTCTCTTTAAACAAAATCATAAAACCCAACCAGAAATTCACTAGTAATGAAGCTGAATTTCTACAGATAGCTACTGATTATTTGGAGGAAAGTCAAAACTATCTTCAAAAGGGTTTAAAGCAATTAAAAAAAGAATATAAAAGATCCATTATTTATAACCCTAACCTTGAATATAAACGCTTTGTTAAATGAAAAGAAAATTTCACTGAAACATTTGAAAGTTATTATGACAGGTTTTTTATTACCAAATACAACCATTATTCACTAAGCTTACTTTTTAGCTTTATTAATGAACAGATTGAAACAGTTATTGCTAGTTACAACTCATTTCTAAATGAGCATAATAAGTTAGCTTTTAATAAAGTTAGTTTTAGTTTTGAAAAGAAACTTTTTGAAGCTACACAACAGTTTAATAACTTAGAAAAAAACACTGCTATTAGTGATGATTTACCGCTCCAGTTTAAAGTTAGAACAACTCAACTAAAAGCCCAAAGAGAAAGGGAATTGAAGAACTTGTTGAATAAAATCAAGCTTAAAAATTTAAGTGAAAAAAAACAAGAAATTTTGTTAAATAACTGGTTTAATAGCAACGAACGTTTGTTTTTAAAAAATGAAGTTAAAAAGGTTAATTGACTAAACTCGCCAAGACAAAAACAACAAGCAGCTCAAATTGATGATCAAAACATTATTGAATTGAAAAATGTGTATAAATACATCACTAATGGCATTACTACAAATGCAGTTCTTAAAGGAGTTGATCTTGCCATTAAAAGTCATGATTTTATTGTGATTTTAGGCCCTTCAGGATCTGGTAAAACCACATTACTAAACATTATTTCAGGGATGGATAGAGCTTCTAGTGGTAGTGTTATTGTCAATGGTTATAACATGATCTGTTTAAATGATAGAAAGCTCACTAAATTCCGTCAAAAGTATGTTGGTTACATCTTTCAACAATACGGTTTATTACCTAATTTAACAGTTAGAGAAAACATTGAGATAGGAGCAAATCTTCAACCAGATCCTAGTAAAAGGATCAGCATTGATGCACTTTTAGAAGCGGTTGGGATGGATAGTTTGCAAAAGAAGCTTCCTAATGAATTGAGTGGTGGGCAACAGCAACGTGTTTCCATTGCAAGAGCTTTTGCTAAAAACCCCTTATTAATTTTTGGTGATGAACCTACTGGGGCACTTGATCTTGAGATGACCCAAATTGTTTTAAAACAGTTTTTAGCAATTAAAAAGCGTTATCAAACGACAATGATTATTGTTACCCACAACAATTTAATTGCTAACTTAGCTGATTTAGTTATCTATGTAGCAGATGGAAAAATAAAATCACTACACAGGAACTTAAATCCTAAACAGGTTGAAGAGATCCATTGAATTTAGATTATGAAATACTTATATGCCACTCAACACCTTACTTTAAATGCTATTAAGCATGCTAAGGGAGGACATGTTGGCATGGCCATTGGTGCAAGTCCTATCTTATTTAGTTTATTTACTAAACACTTTCACTTTGATCCTGACCAACCAAAGTGGATCAACAGAGATCGCTTTGTTTTAAGTGCTGGCCATGGTAGCATGGCATTATATTCAATTTTCCATTTTGCCGGACTTATTTCTAAACAAGAGATCTTACAGCATAAACATGGTCAAATTAACACTTCTTCCCATCCTGAATATGCTCCAAATAACTTCATAGATGCATCAACAGGCCCTTTAGGTCAAGGCTTTGGCATGGCAGTTGGCATGGCGTTAGCACAAAAGTTATTAGCTAATGAATTTAAAGAGCTAAGTGATAAATTGTTTGACCATTACACCTATGTGGTTGTTGGGGATGGAGATCTACAGGAGGGGGTTAGTTATGAAGTTAGTCAAATTGCTGGGTTATATAAATTAAATAAACTAATTGTGCTTCATGATTCAAATAGAGTGCAAATGGATAGTGAAGTAAAAAAAGTTGCTAATGAAAATCTAAAGGTTAGGTTTGAAAACGTTGGTTGGAATTACATCCATACTGATGATCAACTAGAAAATATTGATCAAGCTATTATTAAAGCCAAACAATCAGATAAGCCAACTTTTATTGAAGTGAGAACAACTATTGCTAAAAACACCCACCTTGAAGATCAGTATGGAGGACATTGGTTTATTCCCAATGAAGTGGACTTTCAACTTTTTGAGAAAAGAACAAATACTAACTTTAACTTTTTTAATTATCCAGATAGTATTTACCAATGATTCAAACAAACTGTTATTGAAAGACAAAAACAAATTAAAGAAGATTATAACAATTTGCTAATTTCTCTTAAAGACAAACTACTTTTTAAAAAATTTACTAATTGGATTGACAGTGATTTTCAAGCCCTTTATCTTAACCAACTAGATGAAAAGAAAGTAGCAAAAAAAGATAGTGCTACTAGAAACTATTTAAAAGATTTTTTAAACCAAATTAATAATCCTAATTCCAACTTGTATTGCTTAAATGCTGATGTATCACGTTCTTGTTTTATCAAGATAGGTGATGATAATCTCCATGAAAATCCTTGTTCTAGAAATATCCAAATAGGAATTAGGGAGTTTGCAATGGCAACAATAATGAATGGTATGGCACTTCATGGTGGTATTAAAGTGATGGGTGGTACTTTTTTAGCATTTGCTGATTATTCAAAGCCAGCAATTCGCTTAGGTGCATTAATGAACTTACCAGTATTTTATGTTTATACCCATGACTCTTATCAAGTAGGGGGTGATGGTCCTACTCATCAACCCTATGATCAACTACCAATGTTAAGAGCAATTGAAAATGTTTGTGTATTTCGTCCTTGTGATGAAAAGGAAACTTGTGCTGGATTTAACTATGGTCTTTTAAGTCAAGATCAGACAACTGTTTTGGTTTTAACACGTCAACCCTTAAAATCCATTGATAACACTGATAGTTTAAAAACACTGAAGGGTGGTTATATCCTTTTGGATAGAAAACAACCTGATTTAATTATTGCTGCTAGTGGTAGTGAAGTGCAACTTGCAATAGAGTTTGAAAAAGTTTTAACTAAACAAAATGTAAAGGTAAGAATTCTGTCAGTTCCCAATATAACTTTACTTTTAAAACAAGATGAAAAATATCTAAAGAGTTTATTTGATGCTAACAGTTCACTTATCACCATAGAAGCTAGTAGTAGCTATGAGTGGTTTTGCTTTAAGAAGTATGTTAAAAACCATGCTCATTTAGGAGCTTTTAGTTTTGGTGAATCTGATGATGAAGATAAAGTTTATCAGCAAAAAGGGTTTAATCTGGAAAGGTTAATGAAAATATTTACTTCCCTAAGAAATTAAAATTATCTTAATGTTGTATAGGTTTTGAAAAACAGGATTGGCAATTTTTATGCCTGGTTGCATCTTACTTTCATCCTGTTCTTTTAGAAGTTATATCCCAACTCCTAGTTTAAGAAATACTGTTGGTAACCACAACAGTTATGTTAATAATACTGTCCCTAAAAACAATTTTTATGAAAAGTTTTATGATCTAACTTTTGCTTTAAATTTCACTAATCAGAAAACTCAAGAGTTTGGTACTGGTTGGTTAATTGACTGAAAAGGAGATGAAACTAAAGATCTTAATACATTAACTATTGCTAGTTCTTCTATTATTTCCTCTGTAAGTAATCATTCTTTAAAAGAAAAACAAGATGACAAGCTTTTTATTGCTTATATTGCCACCAATTTACATCTGATAGATGGTTTAAAGAATGATCATGATTATCAACCATACAATAAAGATGGAAATGGTCTTAGTTTTCCATTTGATCAAAAAACCCAATCATTCTTATTGGGTAGGTTTGCCAATCCTAAGATAAATTCCAAACCAGAAGAGATGAACTACCAAGTTCAAACTCGTTTAAAACAAGATGCAATGGTGTTTATCCAAACCAGTACTTTACCTAAAACTGCTTATGCAGGAATTGATCCTATTAACTTTGATTACCATGAAACTAGTGATGAGAGTGGATTTTGAACTAAAAAACAAAGCACTGCAAACTTCCCTAGAACAAGAACATTAAAAAGTTATGCTGATTTTGCAGTTTTAGAGGTACCCTTATTCTTAGATAATGCTAATGATGCCAAGATTTATCAAGAGTGAATTAGACCAGCAGTTCAGGCTTATAAGGAGCTAGGGGATGTTGAAAATATTTTTGCTAAAACCCCATACGCTGAATATATTAATAACACCTACTACTTATTGGGTTATCCTGTTACTAATAACAATAAGTATCAGTTTATCTTAGGTCAAGATGAAAAGTGAAAGTTTTCTCAACAAACTTCTGTATTAAAGCACTATCAAAAACAACCTTTTCAACAAAGAACAGTTTATGTTGAACGTGATGATGGTCTTCCTACATTAACTTTTAATGAAGATAAACTCACTCATGTTCAAGGTACTGATCTAATTAATGTTGATCAGATTACCGATACTAATTTAGGAAATGGCTTAATAAATTATGCTGGTTTATCACGCTTTACTTTAAGCTATCACAATGTTGAATATAAGTTATTTGGTTATGGCACTATTTTAAATAATACTAATTTTCCAGGTGGATCTTCTGGTAGTGCTGTTTTCAACAAGGAAAAACAACTAACAAGTATTTACTTTGGTTCACTGATTAATGTAACAACTGGAAATAACAGGAATGTTAATTTAGGTTTGGGTCAAATTCTTCGTACTTACAACACTAATAATAGTAAGCACAGTGCACCATCATCATATGATTTAATTTTTGGTGATAAAAACACCATCAAGTTTTATGCACAGTTTGCAAAAGAAAAGCAAACTCATCTTTGAAATATAATTCAAACATCTGTTAACTATTCAATCAGCTTTTACAAAGACAAAAAATAATAATTAACTAACAATTTGTATTTGTGTTAAGACGTTATCTAACTCTGAGTTTTTCTAGCTTGCTTTTATTAGCACTGCTGTTTTTAACAGGCTGTTCTTTTGTTAGACCACAATTTCGCAGGGGCTTTAGAACCCAGTTTAAAATTAACTCTATTCCAACTGTTAGTGATCCTTATCACATTAACTACGACTTAACTTTTTCATTAAACTTTGCTTCCAACAAAAGAAATACTTATGGTACTGGTTGGTTAATTGATTGGAAAGGAGATGAAAATAACCCTGAGAAAAATGATCCTTTTAAAGTTTATTTAGCTACTAACCTCCATGTGATAGATGCTTTAAGAAATAATAATGACTATGAACCATATAACAAGGATAGTAATAACCAAGCTTTTAATGGCGAAGAGATCACTAGGTTCTTTTCTATAGGTAAGTACACATATCCTAGTATTTTTAGTGAATTAAATTTCATTAGCAATGCTAGAGAAGCGTTTGTTTCGATTCAAACCTCTACTATTCCTAAAACTGCTTATGCTGCTGTTAACTTTGTTGAAACACAAGGAGAGGATGAAAGTTATACAGATTCATTATCAACTGATAATAAAAGAGATATTTATGCGGATTTTGCTGTGATAGAAATCCCCTTATTCTTAACTAACCATCGCGATTATCAAGTATTTAATGAATTTATTAAACCAGCAATAGAAACATACAAACAACTAGGAAACTCTTCCTTTGAAAAAAAACAACTAGATCAACATAAAAACGACAACTTTTACATGTTAGGTTATCCTTTGGTTGAGAGTAGCATAGATGCTCTAATTTTGAACCAAAGAAGACAGTACAATAACAGTTATACTGAAAAATATACTCCTCAAACTTTAACCAAAGATCAACGTACCATTGACTTAAGCAGAGAAGTTCCTACTTTAATTCAGAACAAAACAGAAAACTCTACAGGAAGTCAATTATTAGTAAATCAATCTTTAAGTAGTACATCTGAGGGGATTATTGAATTTATTAAGTTACCTGAATTTAAACTCAATTATCATAATAAAAGTTATCGTCAATATGGTAGAGGTTTTGCTCTACAAAACACTAACTTTAGACCAGGTTCTTCAGGAACTTTAATGTTAAATAACCAAAAACAGATAGCAGGTATTTATTTTGGTGTTTTAGATTTTGGAGAAGATGTAAGTTTAATGAGTAACATTGGTGTTGGACAAATTCTTCGTGTTCCTCAAAAGAGCAATACAAAAAATAGATCAATTGCTACTAATAAAAGCAACTATGATTTGATTTTTGGTGATGGCAATACAACTAACTTTTATGCAAAATTTGCTAGACAAAATAACACCCATCTTTATCAGATGATTTCAAATAGTAAAGATACAAAATTAAAGTATGTGAATACTGTTGAAAAAACAGTAAAAGCTAGTATTAAATAAAACAAAATAGTGACTTTCTTTTTTTATACTCATAAAAATGGAAAATCAAAACAGTTGTAGTTCTGTAAAAGAAAAGAGTTATATAATCCAAAAAAGCCTTTTCTTTTTGCGGTAATTTCTTTCTTTGTTTTCATAATACTTTTTGCTTTAGCAGGTTTATTTACCAAATTTGCATTAGAAGAAAGCAGAGGTTATAGTTTTCTTGTTATTGTCTGTTATCTTCTAGGAACAGTTTGTTTTTTTGTTTTTATCTATTTTTGGTTTTGATTCTTATCTGAATTTTTTGCAAATAGAGTTCTAGTTAAGTTATGAGATTTACCTACTAAAAAGAGAAGAAAAAGACATGCAAAAAAACAAAGATAATTAGTTAAGTTTTAATAACTCAGTTGGCAAATAAATTAGGGAGTTATTAGAACGCTATTTTTTATTAATTTAAGTGTTTGTTTAAAATTTATTAAGTACTTTTTTAACTTGTTAAAAAAGTAGTTATTTTATTAGTTGGAAAGTGCAAAAAGAATTAAATATTTCAAATGATAAAAAGACTTGTTTAAATGAAATAGACACAAGTAAAGTTAAATTTCTAACAACAACTTTAGTTTTATTGTGATCAATATTAATAACACTCTTAGTGGTTATTAGTGTATTAGCATTTAATTTCATTTTATTAGGATCTACTAAACAAGGATTACCAAATACAAATGAAATAGAGACATTAAAAAGTTCATTAGCTTTGCAATTATCACAAAATGGTGTGATTTTATCAATAGCACTACTTGTTTTTTTTAGTTGAATGGCAATTGTTGGAATTCACAGCTTTTTAGTTGGAATTTTAGTTAATCATCAAACACTTAAAATAAGTAAAAAGTATGTTATTTTAGGTTCGATTTTTCCAATTATGGCATTAACAAATACTCTTGTAATTAGAAAAAAATTAAAAGCTTTATTAGGAGAGGGTAAGGTTCAAAAAGGACTCAAAGTTTTAACTATTAGTTTTATTTGTGTTTGAAGCTTGCAATTGATAATAGGCTTTTTTGTATGACTTTTTCCTTATGCTGGTGAGGCAGGAATTAATATAGGAATTAATTTATCTATTTTTAATTTGGCTCAGTTAGTAGGCTCAGATATAAATGTAATAACTTACTTTACAACTTTTTTAACGCTGCTTTTTGCATTTCTTTCATGAGCCGTATTGCATGTTTTAGCTTGCTTTATTTTGGTACATTTGACTATTTATAAACAACAAGAATGAATAAAAATTAAAGCTATTTACATTTTGGCTTTAACCTTAATTGATTTAATGGGTTTAATTTTGGCTTTTGGTATTATTGTTGTTAACAATACAAATTCTTCAAGTTTTGATGCAAAATCAAATTCCTTAGCATTAATTTTTAAGTTCATTTATTTTGATAGTTCTATAGCAGCCTTTTTAACAGTTTTAAGCTTTGCTAGTTCAGTTGCTTTACTTGTCAATTTAATAAAAAAAGATAAGGGATTAACCTTAAAAAATTAGTTAACCTCCCCCAACCTCTATTTTGTGTTACTAGTGCCTAAGGTGGTAGTGGAGTACCACAAGCTCAGTAAGAAGGTTGTCAAAGAGAGTTTGGAAGTGGAAGCATCGTTCCACTTCAACCCCACCCAAAGGTTGAAGAAAGATAGTCCAGTGAAGGATTCAACAAAAACAGGGGAGAAACTCGAAACAACTGCTTCATCCATGAGTAGTGGTGGGGCTACATCCACTCGCAAGGCCCTCAAGGTTGAGGTGGAGAAACAAAGTGGATCAACTGATTCACTTTTAAAAAACGACTTTGCCAAAAAGCCACTGAAACATAAAGAAAATAGTGGGGAAGTGAAGTTAGATGCGAGTGGGGAGTTTGCGAAGGACAAGGCCTGAAAACCGGTGTTGACTACAGATGAGATAAAAAAAAATAGGGGGATGGGGGCGACGGTGGTTAGTTTCTCCCCTGAATCGGCAATGGTAAAGCTTTTTCCAACTCCCCCTCCCCTTCAACTTCTTCTTCTTCTACTTCTGCTTCTTCTTCTACCCCCCTCCCCACTTTTTCTAACATCAATGTTGGGGTTAAATCAATGATCACTCAACACTTAAACAAAGAAAACACCCGGTGGGTGTTTACCCCTAACTCTTCACCAGACATTTGAACGGGAGCAGGGTATCGCAAGGGTTCATCAAACACCAACGGCATCCCTTTTGAACAGGTGAAGCCTAGCAATAGTAGTAGTAGCAACACGTTTAATCCCAATGATGATGAAAATCAAGTCACTCCAGGTGGCTCCTCCAAACCAACCACCTACACCCATTTACCCAACAGTATCAGTCCCACCAGTGACTGGATCAATGCGTTGACTTTCACCAATAAGAATAATCCACAACGCAATCAGTTGTTGCTGAGAGCACTGTTAGGAACTATCCCGGTATTGATCAATAAGAGTGGAGGAAGTGGGAATGAGTTTAACCATACGAATGATCAGAAGTGGGATAAAACAGATAAAAAAGATGGCAACCTCCCGGGGTTTGGGGAGGTGAATGGTGGTTTTTACCCAGTTTTACTTATTTAATAGTTAAAAAAGCTTTAGATTTTTATCAAATTTTTATCTATTTAATATTTAAGAAAGTCGCAAATTTTTATCAGTTTATTGGTCAAAGAAGTCGCAAATTTTTCTTAGTTTTTTATTTGCTTAATGGTTAAAAAAGCGTTAGTTTTTACCTTTATTTAATTAATAAACTTCGCCACCCCCATCACCGATTCTAAGGCTGATCTGGTGAGTTTAGCACAGTTAGACAGTTCCTATCAAATCTCCGACCAAACCATCCATAACACCAACTTGTTTGTGTTGTTCAAGTCTACTCAGGTAAAAGTTAAGTATGACAGTTCAAGTGGCTCAAATAACCAGATTAGTTTTGATTCAACTAACAACAAACCATCCTACATCGTTGAGTTTACTAACTCTACCAAGATAGGCATCAAGTGAAGCGTGGTGAAAAAGTATCAGTTAGATGTGCCAAGTGTTTCAACAACCATGAACCAAGTTTTGCAAGAATTGATCCTTGAACAACCTTTGACTAAATATACCTTAAATAGTAGTTTGGCCAAACAAAAGGGCAAAACACAAGCAGTGGTGCATCTTGGTTCAAATATGGCAAATCAGTGACAATCGATGCGTAATCAACACAACTTAGACAACAATCCCAGTCCCAATGCATCAACCGGATTTAAACTCACTACCGGCAATGCATATAGAAAACTAAGTGAGTCCTGACCAATTTATCAACCAATTGATGGGACCAAGCAGGGCAAAGGGAAGGATAGTAGTGGGTGGAGTTCAACTGAAGCAACAATGGCAAAAAATGATGCGCCCAGTGTTACTAGAGGGAGAACATCAGATCAAAGTAATAAATTCACCAAGTACCTCAACACCAAACAAGCGTTAGAAAGCATCGGCATTTTGTTTGATGGGGATGGAATGAGGAATGTTGTTAGCCTCCTTCCTCTACTTTCAACCCAACAAGGTGAAAAGTGGTCAATACCAAACCACTAACACCTACAACAGGTTAATTGAGCCTGACAAGTGAAACCAAAGCAGTGATTTGAACAATATGACCAACTTGTTAAAACTACTGACAACTAAAAATGTGAAAGCAAAGTTGGGGAAGACAGCCCAATCTCAGGAAAATAGTGGGGGGGGTGAAATGATAAAAATGTTTTTAAATAACTACATTAATTCTCTTAAACTTACTAAATTATTTTTCCATTTCGAACTATTATTTGAAAAAAGTTATTAATTGCAAGTTGATAAAGAAAATGAAGCAAAATAACAAGAAAGAATTATCTAAATTTAAATAGACTAGAATTAAATTAAGACCAAAATGTTGCATGCAAACAACATATGCTCTTTTAGTTGTTTTTGGAATTTTATTAGTTCTAATTGGACTAGGATTTTTATTTTGAGCTTTAAAAATTTCACTTAAAGAACAAAAGAAAGGTCAAATTACTAATGATTTGACTTCCAATAACTCACTTAAAAATGAAGTTCCTAGAGGGAGTTGATGAAAAAGAAACAACAAATTAGTTTTATTTTTGCTTGCAATAGTTTTTTTAATGCTAGGAATAGGTGGACTTGTTTCCCTTCCTAAGCTGTTTGGTTAATAAAATTTAGTAGTTTTAAAATGCAGATTAGTTTAGTTAAAATCCGCAATAAGTTTAAACAAAGAAACCGTGGTTCTTTTCGTCAGTGAGTTGGTAAGCTTTCCAACGGTTTGATGATCCCTATTGCAGTTTTGCCTTTAGCAGGTATTTTTTTAGGAATCGGTGATGCCATTTCTTCCAATTCATCTGGCATTGTTGGTGTGAAATTTTTTGGTGAATTTATTAAACAAGGTGGTAATGTAGTTTTTGCTAACTTACCTATTTTGTTTGCAGTTGCAATTGCGATCACCTTTTCTCAAGATGCAGGGGTTGCTGGATTTTCTGCTTTTGTTTTTTGGGCCACAATGAACGCGTTTATGAGTTCATTAATTATTCCTGTTGATGCAAATAATACTGCTTCAGGTTATAACATCCTTTATTGAAAAGCAGTACCTCAGTCAGCAATTGCTTCTACTTTAGGATTAAATTCACTTTCAACTTCAGTTTTTGGTGGGATTATAGTAGGGGCTTTAACTGCATATTTATATAACAAGTTTTATGCAATTAGATTGCCTGATGTAATTGGGTTTTTTAGTGGTACTAGGTTTGTTCCTATTATTTGTATGACTATTGCTATTCCAGTAGCATTACTTTTATTGATGGTTTGACCTGGTGTTTCTATCTTATTAAATTTAATAGGAACTGGGCTTGGAATCTTAGGTGGAAGAGGATATGGTGCTAACAGTTTAATCTTTGGATATATAGAAAGAGCACTAATTCCTTTTGGAGTACATCATGCCTTTTATGCACCATTATGATATACAAGTGCAGGCGGTAGTTTGCAAGAAATTGCAAATCAACAAGTTTGGATTAGAGCTCCTGGTAGTGATTATGTAACCAGAGTGATAGGTTGAGAAGATTTTAATACTCCAGGAAAATGAGTTATTCCTGCTGCTTTAGCTAATGGAACAAGTGGAATGATGAATGGAGCTACTACAACAGGACAAGATAGTACATCTGCACTTTCAAAATACATGAGTAAAGAATCAACAAACTTTCTAAGTTGAAAAGAACTTGTTGATGGTCTTACACGTAAAGGTAACTTTGATGAATTGGCTAAAAACGGTTTATTAGATGGTTCTAACAAGATTTGAATTGGTTTAAACCAGTCAGGGATCTTAGGTAAAAAAGTACTGTTAAGTGATGGTAAGGACTACACTATTACCTTTAAAACTTTTGCTAACACCACACCAACATTCTGAAGCCATGGTGCTCATGCACTTTTACCAATTAGTGGAACTCCAAGTGCAATAACTAATGGAGTTACTGTTAATGGTACTGCTAATTCTAAAACCTATAATGTCAGTCAGTTCACTGTTGCAGTTCCTTCTTTAAACCCAGCACAATATTCCCAAGGTAAATTCCCATTCATGCTAATTGGAATTCCAGCAGCTGGACTTGCAATGATCTTAGCTGCTCCTAAGGGTAGAAGAAAAGAAGCTAGTTCTATTATTGGTAGTGCTGCATTCACTAGTTTTCTAACAGGGATCACCGAACCTTTTGAATTTACCTTTCTTTTCTTAGCACCATGGTTATTCTATGGTATCCACGCTGTATTAGCTGCAGTAAGCTTTTGATTAATGAACTTATTGAGTGCTAACGTTGGACAAACCTTCTCAGGTTCTTTCATTGACTTTATCTTGTATGGGGCTTTACCTGATGGTAGGGGTTGATTAGCAAACTCTTACTTAGTACCTATTATTGGTATCTTTTTAGTATTGATTTATTTCCCTACCTTCTATTTCTTGACAATTCGCTTTAACTTAGCAACTCCTGGTAGAGGTGGTAAGTTAATTACTAAAAAAGAATATTTAGCAGCAAAAGCAGCTCAAAAAACTGATCAAACTACTAACACTAACTTTAATCAAACCCAAATTGAAGCTGGTATGTTACTAAGAGCTTATGGTGGAAGTGAAAACATTGCTGAATTAGGGGCTTGCATTACTAAATTAAGAGTAACAGTTAAAAACCCTGAACTTGTTAATGAAACTATTATTAAAGACTTGGGAGCAGCTGGGGTAATGCGTACCACTCCAACATTCTTTGTAGCAGTGTTTGGTACTCGAGCTGCTGTTTATAAATCAGCAATGCAAGATATTATCCAAGGCAAAGTAAATTGAACAGAGTTGCAAAAAGTCTTAGATAAAAATGATAGTACTGTTGAAAAACCAGAAATAAAACCAACCCCAGTTTTAAAAGTTCAAGATGAAATTGTGATCCTCTCACCAGTTAATGGCACCTTAAAACCGCTCACCCAAGTTCCTGATGATACCTTCAAAAATCGTTTGGTAGGAGATGGAATTGCTATCTTACCTAGCGATGGGCACTTCAAAGCACCAGGTGATGTAGGTGTGAAAACTGAGCTTGCTTTCCCTACTGGTCATGCCTTTATCTTTGATGTTGATGGTGTGAAAGTAATGCTTCACATTGGGATTGATACAGTAAAAATTAATGCTGATAAAAAACCAGGGGAACAACTTGAAGTGTTTGATGTAAAAACAAAACAAGGAGAATACACTAAATTAAAGAGTGAAAGTGTTGTTGAAGTTGATTTAAAGAAACTTAAACGAAAGTATGATCCAATCACTCCTTTCATTGTGATGCAAGAATCACTTGATAACTTCAAGTTGGTACCAATTCGCCAACGTGGTGAAATTAAAGTCGGTCAACCTTTATTTAAATTAATTTATAAAGATAAGAAGAGTTAATACAATAAAACTCGATGATAACTAAAAGCCATAAAACCTTGGTTTTGTGGTTTTAGCTTGTTTACAACAATATTATTGCAGTTTTACTGCATAATGTAAAATTACACAGCATGTCAGATACAAATACTGAAAAACCTGAGTTAGTTTCCATTAATAAGTTAAGTGAGATGCGCACTAACATCGGGATGGTTAAACGTTATTGAAACCCAAAGATGGGATTCTTTATCGAACCTGAACGTAAGCATAATAACGATTTATTGAAGCTTGATCTACAGTACCAAGCGTTAAAAACTGCTTATAACTTCATTAAGGATGTTGTTAAAAATCACGGACAAATCCTTTTTGTTGGAACAAAGAATGATTATGTTAAAAAACTGGTAATTGATATTGCTAAAAGAGTTAATGTTGCATATATTACCCAGCGCTGATTAGGTGGTACTTTAACTAACTTTAAAACTCTTTCTATCTCGATTAATAAACTCAATAAATTAGTTGAACAGCAAAAACAAAATGCAAATGATCTAACCAAGAAAGAAAACCTGTTACTTTCAAGAGAGATTGAAAGACTTGAAAAGTTCTTTGGTGGGGTCAAAAATTTAAAAAGACTTCCTAATCTAATAGTTATAGATGATCCTGTTTATGAAAAAAATGCAGTTTTAGAAGCAAACAGCTTAAAAATCCCTGTTGTGGCACTATGTAACACCAACACCAATCCAGAGCTAGTTGACTTTATTATTCCAGCTAATAACCACCAACCCCAAAGTACTTGTTTATTGATGAATTTACTAGCAGATGCGATAGCAGAAGCGAAGGGCTTTGAAACCTTGTATGCTTACAAACCAGATGAACAGATCCAAATTGAAATTCCTCCCAAACAAGAACGCCAAGTTATTAACCGTTCCAATACCAGAAACATCACTAACCAGCGCTTAAACATTAACCGCCAACAACAAGAAACTTTATAGAGCAGTGAACAGTTGAACAGGACTTAGTGAACAAGCGGTAATTAAAAGTCGTCAAGAACATGGTGCCAATTTTCTTCCTGAGAAAAAAGCTACCCCTTTTTGGTTGTTATTTCTTCAACAATTTAAGAGTTTAGTTGTTATTCTTTTACTGCTAGCTAGCTTGTTATCGTTTGTAGTTGCTATTGTCAGTGGTTTGAGAAGTAACTGAAACTTTAACCATGATCTGATTATTGAATGGGTTCAACCTTTTATTATCTTATTAACTGTTTTTGCCAATTCACTAATTGGTTCTATCCAGGAATTTAAAGCCCAGAAATCTGCTAGTGCTTTAAAGTCCTTGACAAAGTCTTTCACAAGGGTTTTTAGGAATGGTGAATTAATTAGCATTAATGTTAGTGAAGTTGTTGTAGGAGATATTATTTTTGTTGATGCAGGAGATATTATCCCTGCTGATGGCAAATTACTACAGGTTAATAACTTACGTTGTTTGGAAAGCTTTTTAACTGGTGAATCAACTCCAGTTGATAAGACTATTGATAGCAATGAAAAAGCTACTATTCTTGAACAGACAAACTTAGTTTTTTCAGGGGCACAAGTAGTTTATGGTAGTGGCGTTTTTCAAGTGGAAGCAGTTGGGATTAAAACCCAAGTTGGAAAAATTGCTAAAACTGTTGATGATAGTGTAACTAAACTCTCACCCTTACAACAAAAACTAGAGAAGATAGGAAAGTGATTTAGTTGGTTTGGGCTTGGTCTTTTTGCTGTAGTTTTTCTTGTTCAAACTGCTTTATTAGGATTTGATAATTTCACTAATAACTGATCAATAGCTTTAATTGGTGCTATTGCGCTTGTTGTTGCAATTATCCCTGAAGGGCTTGTTACTTTTATTAATGTGATCTTTGCATTAAGTGTACAGAAACTAACTAAGCAAAAAGCTATTATTAAGTATTTATCAGTAATTGAAACACTTGGATCAGTACAGATAATCTGTACTGATAAAACTGGTACTTTAACCCAAAACCAGATGAAAGTTGTCGATCACTTTTGTTTTAATTCAACAACCCAAACTGATCTAGCAAGAGCATTGTGTTTGTGTAACAACGCTTCTATTTCCAAAGATGAAAATAAAACAGGTGATCCTACTGAAATTGCTCTCTTGGAATGAAAAGATCGCAGTCAATTAGATTTAAAAACCTATTACAGGGTTTATGAAAAGGCCTTTGATTCAATCAGAAAACTAATGACAGTTGTCGTTCAAAAAGACAACCGCTTCATTGTGATTGTTAAAGGTGCTCCTGATGTGTTATTACCATTATGTAATAACGTTCAAAATGAAGTAAAAAACATTGAAAACTTACTTGATCAAAGTGCTGGTCAAGGCTTGCGTACCTTAGCAGTTGCTTTAAAGGTTTTATATAAGTTTGATCAAAACGATCAGAAGCAAATTGATGAACTTGAAAACAACCTTGAATTCCTTGGGTTTGTTAGTTTACAAGACCCACCAAGAAAAGAAAGTAAGGAAGCGATTTTAGCATGCAAAAAAGCTAATATAACCCCAATAATGATTACAGGGGATCATCTTAAAACTGCAACTGCAATTGCTAAAGAGTTAGGCATTTTAATTTTAGATAATCAAGCAGTTTTAGGTAGTGAACTAGATGAAAAGAAGATCTTGGATTACAGGGTATTTGCTAGAGTAACTCCCCAACAAAAATTAGCCATTGTTAGTGCTTGAAAAGAAGCGGGATTTACAGTTAGTGTTACTGGTGATGGGGTGAATGACGCACCTGCATTAATCAAGAGTGATGTAGGGTGTTGTATGGGGATTACCGGGGTTGATATTGCAAAAGATGCTAGTGATCTGATTATTAGTGATGATAATTTCGCTACTATAGTAAATGGTATTGAACAGGGTAGAAAAACCTTTTTAACTTGTAAACGAGTTTTATTAAACCTGTTTTTAACTTCAATTGCAGGAACAGTTGTAGTTTTATTAGGACTATTCATCTTAGGACAAGTTTTTAAAACTAATTTATTACAACAAGGTCATGACTTTCAGGTGTTTAGTCCTACCCAACTGCTAATTATTAACTTGTTTGTTCATGGTTTTCCTGCTGTTGCATTAGCAGTACAACCTGTTAAAGAAAAATTGATGGTAGGTAGTTTTTCTACTAAAAATCTGTTTTACAACCGCCAGGGATTTGATTTAATCTGACAATCACTATTCTTAAGCTTTTTAACTTTATTGTTCTATAGCTTAGGAATTATATATGCAATTAATAACCGTGATTTACAAACTAGCGGGGATCTAATTAATCGTGCTGGATCAACGTGCGGTTTTTTTATTTTGGGTGCTAGTGCTGCTTTAAACTCATTAAACCTAATGGTAGATAAACCATTGCTTATGACAAACCCTTGGTTTTTTAAGTTAGTTTGAATAGGTTCACTTGCTTCTATACTGGTATTTTTATTGATCATCTTTATCAACCCTTTAGGGTTAGTGTTTAATGTCTTGCAAGATTTAACTAATCACCCAGTTTTAATAGGCTATAGTTTTGGGGGAGTTATTTTGTATATGGGGATGAATGAAGTTGTTAAACTTATTAGATTAGGTTATGGCAATATTTAACTTCCTTAAGTTAATTTCACCCAAAAACAGAATTCTCAGTAAGGCAAATAGGATTGCCAGTGAGGTTGAGAGTTATAAAAACTACTACCGTAACTTAACTGATCAACAGTTATTTGAAGAGTCAAATAAACTAGTTGATCTTGTCACTAAGCAAAATTACACCATTCTAGATGTTTGTGTTGCTGCACTTGCTTTAATTAGAGAAGTGGTTTACCGTGAGACTGGTGAATTTGCATATAGGGTGCAGATCATAGGAGCTTTTATTGTTTTAAGTGGTGATTTTGCTGAGATGATGACTGGTGAAGGTAAGACCTTAACCATTGTTTTAGCAGCATACGTTTCTGCACTTGAAAAGCGTGGTGTGCATGTTGTTACTGTCAATGAATATCTAGCTCAAAGGGATGCTAATAATGCAATGAAGATCTTAAAACGGGTTGGGATGAGTGTTGGTTGTAACTTTGCTAACCTCTCCCCTCAGCTAAAACAAGCTGCATTTAATTGCGATGTTACCTACACCACTAACAGTGAACTGGGGTTTGATTATCTTAGAGATAACATGGTCCACAGTTATCAAGATAAGAAGATCAGAGAGTTGCACTTTGCAATAGTTGATGAAGGTGATTCAGTTTTAATTGATGAGGCGCGAACGCCTTTAATTATTTCAGGTCCTAGTAAAAATGAGTTTGGGTTATATGTTGCAGTTGATCGATTTGTTAAATCATTAACTGAACAGGAGTTTAAGATTGACCCTGAATCACGTGCTGCTTCTTTAACTGAACTTGGGATTAAAAAAGCAGAGCAAACATTTAAAAAAGAAAACCTTTTTGCTTTGGAAAACAGTGATCTTTTTCACAAGATCATGAATGGTTTGACTGCTGTGAAAGTTTTTGAACAGGGCAAAGAGTACATTGTTCGTGATGGCAAGGTTTTAATTGTTGATCACTTTACAGGTAGGATATTGGAAGGGAGAAGTTACAGTAATGGCTTACAACAAGCTGTACAAGCCAAAGAATATGTTGAGATAGAACCTGAAAATGTGATAGTAGCTACCATTACCTACCAATCCTTCTTTAGGCTATACAACCGCTTAGCAGCAGTATCAGGTACTGCTTTAACTGAATCAGAGGAGTTTCTCAAGATTTATAACATGGTTGTAGTACCAGTACCAACTAACCGTCCTAACATCAGAAAAGACCGTTCTGATAGTGTATTTGGTACCCCACAAATTAAGTGAATGGCAGTTGTTAAAGAGATAAAAAAGATCCATGAAACTTCTCGACCTATTCTGATTGGAACTGCTAATATAGATGATTCTGAACTCTTACATAATCTGTTACTAGAAGCTAATATTCCCCATGAGGTTTTAAATGCTAAAAACCATTCAAGAGAAGCGGAGATAGTAGCTAAAGCAGGACAGAAGAATGCAGTGACTATTTCAACTAACATGGCTGGAAGGGGAACTGATATCCGTTTAGGTGAAGGGGTTGCTGAAATGGGTGGTCTTTATGTATTGGGAACTGAAAGAAATGAGTCAAGAAGGATTGATAACCAACTAAGAGGGAGAGCTGCTAGACAAGGTGATAAAGGGGAAACTAAGTTCTTTATCTCACTAGGTGATTCATTGTTTAAACGTTTTGCTCATGACAAGATTGAAAGAGCGATTAGCAAATTAGGTAATGAAACATTTGACAGTGCCTTCTTTTCCAAAATGTTAAGTAGAACCCAAAAACGGGTGGAAGCAATTAACTTTGACACTAGAAAAAACCTGATTGATTATGACCATGTTCTTGCAAGTCAAAGGGAATTGATTTACAAACAACGTGATAAGTTTTTATTAGCAAACGATTTAAGTGAAATGATCGACAAAATGCTAGAAAAGTTTGTACAACAGTTTTGTGATCAATATAGAAACCAAAAGAACCAAAACTTAATTAATCACATTGCACTAGCAGAAGCTTTAAATCTTGAGATGAACATGCAAAACACCATTAATCCAAAGGTGTTTGAAAACATGACTTTTGATGTTGCTGTTGATAAAACCCGTAACTTAGTAGCTAAAAAGATTAGTGATAAAGTTAATGTTTTGACCAAACCAATTGCTTTAAACAGGTTTCGTGACATTATCATAACTTCGATGGATAAACATTGAACTGAACACTTAGATAGTGTTTTTAAGTTAAGAGAAGGGGTTGTACTTCGTTCTATGGAACATACAAGTCCTTTAAATGTTTACATTAAAGAAACAGATATCCTTTTTAAAACAATGTTGCAAAAGATTGCTCAAGATGTCATTGTGCAAATTGCTAACCTCACAACTCCAGATGAATTTGATCATAGCTTAATGCAAGCTAATGCTTTAAAGAAACTAGCAGCAATTAAAGCAGATGAAAAATCAAACCAAGAGCAATAGTTTATTTCAACTTACCACTAACTATATACCTACTGGTGATCAACCTGAAGCAATTAAGAAACTATCAGAATTTAAAACTAAGCAGCAGGTTTTATTGGGGGCTACAGGCACAGGTAAAACCTTTACAATTGCTAATGTAATTCAAAACAGCCAACTCCCAACAGTTGTTATTGCTCATAACAAAACCCTAGCAGGTCAACTCTTCAATGAATTAAAACAACTGTTTCCTAAAAATGCAGTTGAATATTTTATCTCTTACTTTGATTTTTATCAACCTGAAGCTTACTTACCCAGTAAAGGGATCTACATTGAAAAAAGTGCTACAGTCAATGAAGCAATTAAACGCTTAAGAGTCTCAACACTGCATTCACTTTCAACAAGAAAAGATGTTATTGTAGTGGGTTCAGTTGCTAGTATTTATCCCACCTCATCTCCCAGTGATTTTGTTAAGTATTGCTTGTGGTTTGTGGTTGGCAAAGATTATGATTTGAAAACCATTAAAGATAGGTTAGTTAGTCTTAACTATGTTGTTAATAAACAACAATTAACCCCAGGAAAATTTCGCTTCCAGGGTGATGTTTTGGAAGTATTTCCTGGTTACAGTGATGCTTTTGTGATCAGAATCTCTTTTTTTGATACTAAAGTAGAACAAATTTGTCAAATTGACCCACTAACAAATAAGATTTTAAACCAACTCTTTGAGATTAAGATAGGTCCTGCTGATGAATATGTTGTAAACCAATCTGATCTTGATATAGCAATTAAAAATATTAAACAAGAACTTCAGGAACGAGTTAATTATTTCAATAAGCAAAATCTTGTTGAAAGAGCACAACGTTTAGCCACCATTACTAACCATGATCTCAATGATTTGAAGGCTTGGGGATTTTGTAGTGGAGTTGAAAACTATGCTAGACACTTAGAGTTGAGGATGGCTAACTCAACCCCTTACAGTATCTTTGATTATTTTAAGGGGGATTGGTTATTGGTTATTGATGAATCACACCAAACTTTACCGCAACTTAATGGGATGTATAACACTGATCTTTCAAGAAAACAAAGCTTAATTGATTATGGTTTTCGACTCCCCTCTGCACTTGATAACAGACCGCTCTCATTTGCTGAATTACAACAAAAAATGCAAAAAGTTATTTATGTTTCAGCAACTCCAAGAGATAAAGAGATTAGTTTAAGTCAGAATAATGTCATTGAACAGTTAGTTAGACCAACCTACTTGGTTGATCCTATTATCGTTGTTAAACCAAAAGATAACCAGGTGGAGGATCTCATTGAAGAGATTATCAACCAACGCCAAAACAACACAAGAACATTTGTTACTGTTTTAACTATTAAGATGGCTGAAAACCTCACTGAATACTTAAAGGAACGCAAAATTAAAGTTGCCTATATCCATAAGGACATTAAAGCATTGGAACGTTTATTGTTAATTAATGACCTGAGAAGAGGTGAATATGAGTGTTTAGTTGGGATTAACCTTTTAAGAGAAGGGTTAGATGTCCCTGAAGTTGCTTTAGTTTGTATCTTTGATGCAGATATCCCAGGACTACCTAGGGATGAGAGAAGTTTAATCCAGATTATTGGACGTGCTGCTAGAAATGAACATGGTCGAGTTGTTATGTATGCTAACCATGTTACTGAACAGATGCAAAAAGCCATTGATGAAACCAAAAGAAGAAGAACTGTTCAAATGGAATATAACAAGCTACATAATAAGATACCAAAAACAGTTGTTAAACCCCTTAGCTTTGTTCAACCAATCAAATTAAAAGCTAAGAGTAATGCAGAAAAAAATGCTGCATTAATCAAACAATTAACCAAAGAAATGAAGAAAGCAGCAGCTAATCAAAATTATGAACTTGCCATTGAGATTAGAGATTCCATATTTGAATTGGAAAAAGAAATTGATAGTAAAATTAAAGTATAGCAATGCGACAGTTTATTAAGCTTAGTCTCTTAGTCTTTGTCTTGCTTTTCTTAAGTGAACTTATCTGTAGGTTCTCTTTACGCTTAGTAAATTCTATTAAAGCGAGATATAAATCTTCAGTATTTTCCTACACTGCTTGTTTGCTATTTTTAAAGAGTTTTCAAAACTTTTCTAATGCTTTTCAGAAGTTAGCTAACTGGGTATTTTGATTTGAAAATGATGTTAATGAACTGTTATCTATCTTTTACTTTAACTTTGATCAGAAGAGTGAAAAAGTAGATTACAACTTCTTTAATGGTTATAAAGTTACTGCTCAAAAAGTAGTTGAAAAAGAACAGTTATTAACTTGTAAGTTAAGTGATTACTACCGTTTATTTAGAGATAAAACCTTTTGATTTGAACTTATCAACAACTAATAAGATCCTTATTGGCTGTTTAAGTTTGCTCTCTTTATAAATTCATAACTTTTCTCTTAACATATGAAATTAAGTACAATAACTACTATCTGCTTGTCAATATCTGGTGCTTTTGGTACTACTGCAATTGCTTTACCAACTACAGTAGCATTGTTAAAAAACCACCAACAGCAAAACACTGAAAAACAACAAAACCCGATTAAAGATATCCGCTTTGGTTTAAACAATGTACAGGTTCCAAATACCATTCCGTTACACCAAACTGTGGTTGAAGTGATCAACAACAAAGAAATTGTTGATTACAAAGATGCACCCCAAAAACTCTTTTTAGCTAAAAGTGCGTTAAATAATAAACTCCAAGTTGAGTTTGATAAGTTCTTGCTAAGAACAGGAGTAATTAATGCTTTAAATGCAGATTTGAAAGAATGGATAGACCAAACATTATTTATCCCCAATCAAAGTTTTTTTGATCTTAGTGCTAATAAACTCAATTTAACTTTGTCAAATCAAGGCGAAGTGAGTTTAGATCTTGATTTTACTTTTACTAACTTTAGTGATAAAAATCAACCATTAAAACTCCCCTTTGACGGTAATGTGGTGGTTAATGCTAACGAATCATACACCTATTCAGTTAAAGCAACACTACAGAAACTAAAAGTATTGACTTTTTCAAGAGCAGATCATTATGTAGGGATTAGTTATGCAATACCAAAAGTGAGTTTAAATGGTAAAACTCAAAATGACTTTAGTTTTAACCCTTTTAAAAGTAATATTAACTTTACTTTTAAAAATGTCTACAATGCTTTAGATCCGTTTGAAGCACAAAAATACCTGGTTGGCCAAGGCAAGTTTTTAAACCAAAAAGTTAATGCTGATGATGTTAAAAATGACATTAATAACCACATTGAAACCCAGTTTAATGTTGCTAAAATCACAGCAACTCTTTTAGGAAAAGCTTTTAAGCAGTTTGGTGAACATAAAAATGGTCAACCACTTTCACTTTTAAAAGTACTAAGTGGATTAAACAATGAGTTTAAACAACTCTTTAATTATGTCAGACCTGGTTTAGGTGATTTTGTTAGTGATTTAATCCAAAGCTCCAGTCAATCAAGTAATAAAAAAACTGTTTACCAGCTATTATTTGAAAACAAGACAACAATTATCCACTTGTTGCAAGATCTTAATATTAGTGAACTGAACTCTGTTTTACCAGTTGTTGATATTTTGTTTGAAGGGATTAACAGTGCAGAATCACTATACCAAAGGATCCAATCATTTAAGGATTTAATTGTTCCTGCTTTAAAAGCTGATAAGCAACTTAAGAGCTTAGAAGCGATTATTTTAGCAGTTTTAGACAATCCTAATACCTATGTTTTTGATCTTGTTTACCAAAACAAATCGATTCTGTTTAATTTGTTAAGTGATTTTCTTAAAAACACTGCAAACACTTTACCATTTCTTCAAGAACAGTTTGATATTGTTAACCACTTATTTGCCAATGAAGCGATCTTTGATCTGTTTTCCAATGCTGACTTTGTTGATAAGATTGCTGATCTTTTTTTAGCAAAACAAAAGGTACAAGAAGTTAATAATGATGGAACTAAATCAACAAAAATTGTTGATTCGATTTTAGTTGCTACCCTTAAGGGTTTAGTTGGTAATCAGCTCAGTTCAATCACTGAACTGTTAAACATCTATATCTTTGAAAATGAATTTTTAAACAGAAATGATAGTAATAGTTCAGTTAAAAAACAACAAACTGATAGTCTAAAAAATCTGTTTAGCGTAATTGGTGATATTTTAAGTGAGACCAATGTTAACAAGATCACTTTACACGCAGTTAAAAATAATGAACTTCTTTCGTTAGTTGAAACAGCTTCAACACTAAAAATTAAACATCTTAATGTTCAATACAAAGTTTTAGTTGATAAGTTTGAATTGAAAAACAGTTTCATTAAGGCGCTATTAAACTTTTTCCCAGATACTAAAGATATCACCCCAACTATTAAAAAGGTGCTGTTTGAAAGTGAAAACTATAAAACACTCCGTAGGAAGTATGAAAATGAAGGGTTCCCAGGTTATCACTGGGCTAAGTTCATTGTCCCAGGTACATTCAATTCTGCTGAAAACACTTTCTATTCTGCTATTGATAAAACTAAATCAATTCGTGATCTGTTTGCTGACATGTTATTTGGTAAGAGTTTAGAGAGTGTTAATGACAATGATAGCTTTATCAAAATTAATGGTTCATTTACATTAAAGTACCACGGTGATAACTTAAATTTACTCCCTAACTACCACTCATTAATCACTAAGAATGTTGGTTATCAAATAGTTAATGTTAATTTTCATATTGATGCAAGATTGCTAACTGCAGAACTACAAAACACGGTATTTTCAAATCCAAAACCAGTTATTAAATCACCTGTTGAACTCTCCAAATCCTTATTTGAAGTTTGAAAGACTATCTTTGAAAACTCAGTTAACCAAATCTTAAAAAAAGAATATACTTCCAAAGATAATCTAAAGTTCTTTCCTTTTAAAGCTGATGGCTCCTCACGCTTAGAATTTGATCTATCAAAACCTGATCAACGTGTTATTCCTTTTGCTTTTGTTGATGGTTATCAATTCCAACTAAAAAAAGAGTTAATTCCTAACAAAGAAACAAAAAAAGAAGCTAATTCTTCACCAGTTTTAAAGCTTTATGATGCAGTAAGAAACGATAGACAATATCGTGCTAACCACCACCATGATGATCTGAGAAACTATCCTAGTCTTAAATCCCAACTAGAACTTATATTAAACCTTGGTGATAAGCTTAAAGCTAATAATGATTTTATAGATGACACTGTTGTTAATGCTTTGCAATATAAAACTAGTTTTAAATCTACTCTAAAAGTTAACAGTTTAGGAATTCCTATTGACCTTTTCTTTTTCACATTGTGACTTAAATTTAACTTAGAAATTCCAATTGACGGTTCATTAACATTAACATCTGTTAATGTTGTTTTCCCATACAGTTTGTATGATACTAGTTCAAATGAATTTACAAGGATAGTCGATCGCTTAAATTTCACTGATACCAACTTCTATCTAAAAGATGCATTTCCTAATTTCTGGTTTGTTGGTTTCTAGAGTAATGAAACTAAACCACTAACTAAACTACCAACTAGATAAACAATCAAGTTCATCCACCAAAACCCACTAGTTTTTTGTCTGTAAGTTGCACTTTGATCTTGATATTGATTAAAACTAACTAATGGTTTTGCATAAACACTACCTAACTTAAGATATCTGTTAAACTTACTAATTTCATACCGCATTCCATCACAAGTTTTTAAACGGAATATCCCCTGTAAACAAACAAGTAATAAGTTAACAAAAAAGCTAGTTAGTACTGAATTTTTAACAACCACTGTAAAATTGGTTGACTTGTGAAAATAAATAGTAATGTTTAAGATCAAAAAGAAAAGGATTAGATAAAATACGCTAACAACTATCCCACCATACTCAGCTTTATTACTGTTTTTGTTTTGATTTAAAACCATCTAGTAATCGTTATTAGTATCATTTTTTTATAAAATAATCAAGAACATCAGCACATGATCTTTATCACTAAATTTATGTTTGGTTTCAAATGAAGTTGGATTATAAATCTTGCTTTATTGTAATCATGCTTTAGATTTAGAAGCTGGTTACTTTTGTTTCAGGTAACCAGTACCACTTCCAAAAAACTGAGAACCTGAAAAAACAGGTTCTTTTTTATTTCTATTTAGTAAATGTTCAAGTATATTCTTAAACGATTAGGACTAGCAGTAGTTGCGATGTTTATCGTAATGTCTATAGTCTTCTTTTTAGTGAACGCTACTGGTAATGTTCCCTTGTCAGCCACTTCTGCAAGAGATATTGCTGCAGTGCAAGCACAACTACAAGAGTTTGGGTTTAATGACCCTATTATAATTAGGTATTTTCGCTATTGAGCTAAGCTATTTTCCTTTCAAGCTGATGCTTTAGGAATTTATTATGCAAACCCTAACCAAACAATTGGTGAGATTGTGTTTGCAAGAGTACCAAATACCTTATATGTGGTTTTAATCTCTTTTTTAATTGGTTCATTGCTAGGGATCTTTTTAGGGATGGTTTCAGGATTGAATAGAGGGAAGTTTTTAGATGCAGCAATTAATGTGTTGGTAGTTTTATTTGTATCTATTCCTTCATTTGTAGTGGGATTAGGGTTACTTAAACTAGCAGGATTTTTAAATCTACCACCACGGTTTATTAACTTTGATGATGCTTTTTTTAGCTTTGATCGTTTCTTGCTTGCATCAATTATCCCGATCCTTTCATTGGTCTTTTATTCATCAGCTGCTTTTACATACAGGATTAGAAATGAGGTGGTGGAAGTGATGAATCAAGACTATATTAAAACTGCAAAAAGTAAGGGACTTGGGATGTTTGCTGTAGCTAGGTATCATATCTTTAGAAACTCGATTATTCCTTCTATTCCCTTGTTTGTATTTGGAATCTCAGGTGCTTTTTCAGGTGGATTTATTATTGAGTCTTTGTTTGGAGTACAAGGGGTATCTAGGATCTTAATTGATTCAGTGCAAGTTAATGAAACTAACATGGTAATGTTTAATATCTTGTTTATCCAAGGGATTCCCTTATTAGCAAGTGTCTTTATTGAATTTATCTATGTTTTAGTTGATCCTAGAATTAGGATTGCAAATAGTTCTAATGTTAGCTTATTAACTAAGTTAAAGTTCTTAAGTTCAAGACACCAATGGTTAATGAAGTGAAACAAGATTAACAGTGATAATGCCCAAAATATTGTGTTTAACTCGCCACTGCACCACCAGTTATTAGAACTCAATGCAATTGATTACAAAACAAAAACAGTCCAACTAACAACTGAACAAAAAACTGCTCTCAATATCAGTGCAACTGCTAACTTTATCTTACTTGGTAACAAGTGTTTAAAACTCAAAACAATCCATGGATAGAAATAAAAGTTTTGACCCTAACTTATTTAAAAGGGTTGATATCAACTTAATAAAGCGAAATGATCAGCTTATTGGTAAACCAACTACCAATTCAATAGAAATTATCAAGCGCTTGTTTCAAAACAAGTGGGCCATCTTATTTTTTCTTTTAATAGTTGTTATTGTGCTATTAGCAATTATTGTGCCTTTAACTTCCCCTTTTTCAGCAGTAACTCCTGTTTCAACCAATGCCTTAGCACAAAATCTACCACCACGGTACTTATGGCATAAACCAGGTGACATTTTAGTTCATAAGATTACAGCAAGATCAATTGCTGAAATCTCTCAAGCTAGTGGAGTTTTAGTAGGAACATTACCTAGTGCAAATAGTAATCCCTTAGCAACTAATGTCCAGTATGATATTGCTCCTTTTCAACTCCAAGAATTACGTAATTATTTCCCTTTATTGGGGACTAATGGACTTGGGATTGATATTTGAACCTTGTTGTGAGCTTCTGTTGCCAAGTCATTGTGAATTGCAGTAGTAGTAGCAATTATAGCAATGGTATTTGGAACCATTTATGGAGCGGTTGCTGGAAGCTTTGTTGGACATATGGCTGATAACATTATGAGTAGGATCATTGAGATTATTGATATAGTCCCTTCTATTCTTTGAATTATTGTTTTAGGAGCTACATTCCGCTTTGGTGGGGTTAAACAATTTGATGATAGTGTTGTAATCTTTACTTTGATCTTTGTGTTTTGAACATGACCTGCTACTACAACCAGAATTTACATTTTGAAAAACAAAGATACAGAGTACATCCAAGCAGCTAAGACCTTAGGGGCACACCAAATCAGAATTATCTTTGTTCATATGTTACCTGTTGTATTTGGGAGATTAGCTGTTGTGTTTGTTAGTTTAATCCCAGCAGTTATTGGTTATGAAGCTTCCTTAGTTTTCCTTGGGTTAAAACCAGCTACTGATATTGGCTTAGGGGCACTTTTAAACCAAGTAACTTCAAGTGATAATGTAGCTTTAATCTTAAGTTCGATTGTTAGCTTTGCAGTTTTAACAGTAGCAGCTAGAACATTTGCTAATGCTTTAAATGATGCAATTGACCCTAGGGTTGTAAAACGATAAAATGGCACTTAAAAGAAGTAATTTCTTTGTTGATAAAGACCAACAACTAAAGGATAATTTGATCTTAGACATCACTGATTTACATGTTAACTTTAAGGTTAAAGATGGGATCTTACATGCTGTTAGAGGGATTGATCTTAAGGTAGAGAGGGGTAGTATTGTAGGGATTGTAGGTGAATCAGGCAGCGGTAAATCAGTGAGTGTTAAATCAATTATTGGTTTTAATGACAATGCACAAACTAAAGCCAAACTGATGAACTTTAAAAACGTTGATATTACCAAACTAAAGAAACACCAGTGGAAGTATTATAGAGGGACATATGTCTCTTATATTTCCCAAGACCCATTGTTTTCTCTAAACCCAACAATGACGATAGGAAAACAAGTAAAAGAAGCGATTTATGTGGCTTCAAAAAGAAGGTATTTCCAAGCTAAATCAGACTTAAAATTTGCTTTATCAAATAAGGAGATTGACAAAAAAACTTATAAAAGTAAACTAAAAGAGATCAAACAAACCTACCAACAAAAAATAAAACCTATCAATGTAGAGAAAAAAACCTTAGAGATCCTGCAGTTCATTGGTATTAATGATGCCAAGAAACGTTTAAAGGCATTCCCAAGTGAGTTTTCAGGAGGGATGAGACAGAGAATTGTGATTGCTATTGCAGTAGCAACTGAACCTGATTTAATTATTGCTGATGAACCTACTACTGCACTTGATGTAACTATTCAAGCTAAGGTATTAACTTTAATTAAACAACTCCGTGATTTACTTAATATCACTATTATCTTTATTAGTCACAATATCTCTTTAATTGCTAATTTCTGTGACTTTGTTTATGTTATGTATGCAGGGAAAATTGTAGAACAGGGTCTTGTTGAAGAGATCTTTACAAATCCACTCCATCCCTATACATGGGCATTGATTTCTTCAATTCCTGAACAGAAAGATAAAAACAAACCACTAACTTCTATCCCTGGAGTTATTCCTAACATGTTAACCCCACCAAAGGGTGATGCTTTCGCTAGTAGAAACCAATATGCTCTAGCAATTGACTTTGAATACCATCCACCCTTTTTTGAAGTTACTAAAACCCATAAAGCAGCAACTTGATTGCTGCATCCCCAAGCCCCTAAAGTTGAACCACCTCAAGCGGTTATTGATAACATTACCTTAACCAAAAAAGCACTGCAATTTAAAGATCAATAATGGAAAACCAAAACACAAAAAAACCACTTGTTAATGTTAAGGCTTTGAGCATGATGTTCAAGGTCAGAGGAACTCTTTTTAAAGCCCTTGATGAAATTGGTTTTACTGTTAATGAAGGGGACTTCTTTGGAGTTATTGGTGAGAGTGGTAGTGGTAAATCAACCACGGGAAAATGTTTGATTAGATTAAACATTCCTAGTGGTGGAAAGATTGAGATTGCCAACCACTTACTCTCAGGAAAAAAACTTACTAAAGAGAATAACCAGTGGTTAAAACAAAACATCCAAATGGTGTTTCAAGACCCTTATTCATCTATTAACCCTACTAAAAATGTGCTAACTGTGATTTCAGAACCGCTGGTAATTAGTAAAACTGTTTTTGGGGAAACAAAACAATACTTAAAGAGTTTGCAAAAGCTCTCTTTTAAAGTAAAGAAAACATTGTTAAGGAATGATATTGAACTTGAAACCAAGTTTCACAATAACTTTTTTAAAACCGTTATTAAGCAAATTAATGAATCATTGTTTAACTTTGAAGATCTTGATTACAAGGATTTAAAACCATCACATTTAAGGCAAAGAATCATAAATGAAACAGATAAATTCATTGAAAAAATTAGAAGTGAGTTTGCCCTTTTTTATGATTTTTATGCTAACCAATCAGTACCCTTGCAAAAGGCATTAGATGATGCGAATTCCTCTTTAACACCATCTAGTGTTATTGAGTTAAAAAACCAGTTAAAAGCATTACAAAAACAAGCAAAGATTTCAAAGGCAGCATGGGATATTTTACAAGCCCTAAAGCAAAACCAAAAGGAGTTGAAAGATTATGAAAACTATGTCCATTTTGAACTCCAAAAAAAGCCACGAATCTATCTTAATACCTGGCTTTTAACAACCAAAAGCTACATTAAAGATTCCAAGCAAAACATGCAGCTTACTGATGATATCTTTGCTTTTTCATACAACAGTATGGTTGACAAGAAAAGAAACTTGGTTTTAATTCTTTCTAAATACTATAAGCTGTTACCTTATTTCTATGACCAATCAGTATTTGATAATGCTGATCAATTTGATGAAATTGCTAACCTTATCTTTTTTGATTTAGTTGAAACATTGCTTGGTGTAACTAGTTTATTTAATGATGCATTAGCAGCTGATAAAGTCCCACTAATTAAGTTTGCTAAGTTCTTAAATAAGTTATGTGACTTGCGCTTTTTAACCTTAAAAAAGAGCTTTAAAAAAACAAGAGTAAGTTGTAGCTTTAGTTTTAACAGTGAACCTGAAATCTTGTTTGCCAACAGCTGCTATGATTTGCAACAAATGCCTCAAATCATTAAACCCTTTTGAGAGAAGCTTTTTAATGAACAGAACTACCAAAAGATTATTGATTCAGTTTCAAGACTGAATGTAATGATTGCAAATGACATTACCAAAGCTTTTGAAATTAAAAAAACTATTGATGAAAAACTAAGGGAGTTTAAACAACAAAATTTAGCTTTAAAAAAAGCTTATTCAGCTAACAAGAAAAGTGAGGCAAACAAAGCTTCCATTAATGAGTTAAAAGTCAATTTAAAAACACTTAAAAAACAGCTTAAACAAGAGAAAAATACTACTAAAAAACAATCAAAAAAGGAATTAAAACCACTTTTAAAAGAACACCATACTGCTTTAAAACTCCATGATGAGTTTAACCATGATTTACGCAAGTGGTTCAAAAAACTTAACTTTATGGTTAAGAAATACAACCGACTGGAAAACAGCCAGAAAAAGTTTTGTTTAGTTAAAAAGTTAAAAGCGCTTTTCAAAAAACAGGATGAAACACTACAAAGTGAATTAAGACCAAAACTAAAAACATTTGGTGTAATTAACTTTGAGTACAAACGTGCAGTCAAAGAGTCCAATGTCTTTCGATTGATGCATTTTGCTAAAAATATCTTTAAACCATTCTTGTTTTTTAACCTCACCAAGATTTTTATGAGAAATAAGGTCTATGAAGCACTCGATAGTGTTGGTTTAAAAAGAGAACATGCTTACAGATACCCCCATGAATTTTCAGGCGGACAAAGACAAAGAATTGCTATTGCCCGTGCTTTAATCACTAAACCCAAACTGATTATTGCAGATGAATTGATTAGTGCACTTGATGTTTCTATCCAAGCCCAAGTTATTAACATCTTGAAAGACTTGGCTAAAAAACACAACTTAACTGTGCTTTTCATTGCCCATGATTTATCAATGGTGCAAACTGTTTGTAACCGTTTGATCATTATGCATAGGGGCAAGATTGTTGAACGGGGCAGTGTGGATGAGATCTTTTCAAATCCAGTTCATCCCTACACCCGTTCCCTAATAAAAGCATCTCCTAAGTTAAGCAAAATCAATGTTGATCTCGCTTCTTTTGATGAAAACTTCACTTATGATAGTGATTATTCACTAACCAATATGCCCTTTTATATTAAAGTTCCTAACAGTGAAGAACATGAACTTTACTGTACTCAAAAGCAATTTGATAGTTGAATCAAAGAGGCTACGCCGATAAATTAATTTAAAATTTTCCAAAAATGTGGGAGCTAATTAAGCGTTTGTACCACAGCAAAATATGGCTAAAAAAACAGTTACAAGAATCGCTAAGATTAACCTAATTGGCGGACAAGCAAAACCTGGCCCTGCGCTTGCTTCTGTAGGGATTAATATGGGTGAGTTTACCAAACAATTTAATGAAAAAACCAAGGATAGACAAGGTGAAACGATCCCTTGTATAATCACTGCTTTTAACGATAAATCATTTACTTTTGTCTTAAAAACTACCCCTGTTAGTAACTTAATTAAACAAGCTGCTAAACTAGAAAAAGGTGCTAAAAATGCAAAAACTATTGTTGGAAAAATCTCCTTACAACAAGCTAAGGAGATTGCGCAATACAAGTTAGTTGATCTTAATGCTAACACAGTTGAAGCAGCATTAAAAATGGTGTTAGGTACAGCTAAACAGATGGGAATAGAGGTAACTGATTAATGAAAAAACTATCAAAAAGGATGCAAGCTGTTACCAAGCTCATTGATAAAAACAAACTTTATCCTATCCAAGAAGCATTTGAATTAATTAAAAAAACAGCAATTACTAAGTTTGTCAGTTCAGTTGATATTGCTGTTAGTTTAAACCTTGATACTACTAAAGCTGAACAACAGTTAAGAGGTGCAATTGCTTTTCCTTTTAGTATTGGTAAATCTATCAGAATTTTAGCTATCACTGATGATGAGAAAAAAGCTAGTGAAGCAGGTGCTGATTTTGTTGGTGGGCTTGATAAGATAGAAGCGATAAAAAATGGCTGATTAGATTTTGATCTAATTATCACTTCTCCCAAGTTCATGGGAGCATTAGGTAAACTAGGAAAACTATTAGGAACCAGGGGATTGATGCCAAACCCAAAAACTGAAACAGTTACTGATGATGTAGTTAGTGCTATTAAAGCTTATAAAAAGGGTAAGAAAGAATATCGAACTGATTCATTTGGCAACATCCACCTCTCTTTAGGTAAAACAGATACCAAAACTGAGCACTTGGTTGCTAATGCCATGGCTTTAATAGATTTAATTAAATCTAAACGTCCTAGCACAGTCAAAGGTACTTACATTAAAAATATTGCTTTGACAACAACAATGGGACCAAGTTTAAAAGTAAAGCTACCTGATTAATGCCCACCTATAAACTAATTGTTGGTTTAGGTAACTTAGGTAAAAAGTATGAGAAAACTCGCCATAATGCTGGTTTTATGGTGTTAGATAGACTAGCTAGTTTATTCCACTTAAACTTTGATAAAACCAACAAGTTAGGTGATTATCTTTTTATTAAAGAAAAAGCAGCAATCTTAGCAAAACCTGCTACCTTTATGAATAATAGCGGTCTTTTTGTGAAATGGTTACAAGATCACTTTCAAATTCCGCTTGCAAACATAATGATAGTCCATGATGAAATAGCGTTTGATTTGGGAGTAATTAGGCTTAAAATGCAAGGGAGTGCTAACAATCATAATGGCATAAAATCAGTAATTAGACATTTAGATACTGAACAGTTCAATCGTTTACGCTTTGGGATTAAATCACAAAATACGAGTAACATATTGCATGAACAGGTAATGAGTGAATTCCAGAATAGTGAACTGACTAAACTGGAAGTTGCGATTACAAAGTCTGTTGAACTGTTGAAGCGTTATATTGAAGGAGAAGAGTTACAAAGGTTAATGGAATATTATCATCATGGCTAGTGAAAAACAATATATAGCAGGGGTTTCAGGTGGATCTGATTCAATGCTAATGCTTAAACTTTACCAAAAGAAGATTGCTTGTGTTGTTCATGTCAATTACAACACCAGATCAACCTCATTACGTGATCAAAAGTTAGTAGAACAATACTGTCAAAAATTAAATATTCCACTGGTTGTTCACACTGTTGATCCTGATCTAGTTTGAAAGAAGAACTTTCAAAATCAGGCACGGAAAATCCGCTTTGATCAGTTTAAAAAGACTGCAAAGCTATACCAGACCAACAAGTTATTATTAGCCCATCACCGTGATGATTTCATTGAGCAGGCCAAAATGCAGCTGGATGCAAAAAAACGTGCTGTTTACTATGGGATTAAAACCAGGTGTGAATTGTATGGTTTGAAAATCTACCGTCCCCTAATGAAATACTGAAAAGATGAAATCATTGCCCTCTGTAGACAAGACCATATCCCTTATGAGATTGATGAAACTAACAAGTTACCTATTTATAAGCGCAATGAAGTGAGGTTAGAGATAGAAAAATGGTCTAAAATCGAAAAAGAACAGTTTTATATTGCTATCTGTGCAATGAACAAAACAATTGCTCAAAAACTGTTTGTATTAATGAAAAAAGCTAAAAAATGGTTATTACAACCTGATGTTAGGGAATTGAAACGGTTTTCAATTATTGATCAAAAACAGTTAATTTATAGCTATCTTATCTATCACAAGATTAATGTTAATGGTGAGAAAATTGATGCTATCCTTGATTTTATCCAACCTAGTCAACAAAAACAATACCGCTTGCAAAACGATATCTTTTTAATGGTGAAAAACCAGTGTTTAGCACTTTTATACAAAAGCTAAATGAAACATTTAACTGTCAAAGCATTAGTTTTGCAATTTAATGATTGTATCCAACTCATTGATGGTAAAAACAACATAGATAATGTGATTACTATCCCTGGGTTAAAAAGGAGTGTTTTTGAACTATTAGGACTATTTTGTAAGCCAATTGGTTCAGTTGCTATTTTAGGTAAACGTGAATTTATTTTTTTAAATCAAAAGCCAGTTGAGCAACAGAAAAAGATTATTGCAAACCTCTTGAAACTCAAACCACCTGCAGTTATTCTAACCAAGTCATTTGTTGATTGTGGTGTTTTGTTAGCTGTTAATCAAACTTATCAAGTTCCTATTTTAAAAACTAACTTGTTTTCAACTGAACTCTCTTTTACTGTTGAAACCTATATTAACGAGCAATTTGCTACTGTTCAAAAGTTACATGGAGTTTTACTTGAAATCTTTGGTGTTGGGGTATTTTTAGAAGGAAAGAGTGGGATTGGTAAATCTGAAAGTGCTTTAGATTTAATTAATAAAAACCACCTTCTAATAGGTGATGATGCTATTGAGATCTACAGATTAGGCAATAGGTTATTTGGTAGAGCACAAGCACTGGCAAAAGGCTTTATGGAGATTAGAGGTCTTGGCATCATTAATATTGAACGTGCTTATGGGTTACAGATTACAAAAGAACAAACTGAAATCCAACTAGCAATTAGTTTGTTGAGTTTAGAGGAGAAAAACAACGCTAGTTTTGAGCGCTTAGGCAGTGATTTAAAACTAAAAAATCTGCTTGGGGTTAAGATTAGTTACTATCAGATCCCTATCTCTTCAGGTAGAAAAACAAGCAAAATTATTGAAAGTGCAGTAATCGATTTTAAACTTAAGAAAAGTGGTTACAATTCAGCAAACGAGTTTATCTTAAAGCAACGTGCCATGTTAGAAGAACAAACTGATGAATAGACCAAGTTGATCAACTGCATTTAATATTGGTGGTGGATTTCCCATCCAGTGGTATGGGATCATTGTCTCAATTGGCATTATTTTTGCCATTTTAATGTTTGTCTTTAAACTGATTTATTGTTACAAATTACAAGACAACAGTTTTTATTTTTTTATCTTTATTGCTGTTTTAACGATGGTTTTAGGCGCTCGCCTCTGGTCATTTGTAATTGGTGATTCCAATTTTGCTAACAACAATTTCTTTGATTTTCGTAACGGTGGATTGGCCATTCAGGGTGGGATTTTGTTAACCAGTATTGTCGGAGTAATCTATTTCAACTTCTTTTTAAATAGTAAGACCAATAAAACCAAAACGATTGCTGAATTACTGAATAATAAGAATGAAATAAAAGCTGTTTATGTTGAAAGAAATATCTCTGTTCTAGTGATGTTAGATCTGATTGCTCCTTGTGTTTTAATTGGTCAAGCAATTGGCAGATGGGGTAATTTTTTCAACCAAGAAGTTTATGGGTTTGCTTTAGCTGGAACAATGAATGATCCCCAAGCATTGGCTAATACCCAGTGGGGATTTTTAAAGATCTTAATGCCTAAGGTTTGGGATGGGATGTGGATTGATGGTCAGTTTCGCATTCCGCTCTTTTTAATTGAGTCATTTTTTAACACTATTTTCTTTGTGTTAATTTACTTTGTAATGGATTTTATTAGGGGAGTTAAAAGTGGCACAATTGGTTTTAGTTATTTTCTTGCTACTGGAATCATTCGTTTAATCTTGGAAAACTTTAGAGACCAAACCTTTTATTTTCAAACTTCAATAACCACTAGTATTTTGTTTATTGTCGTTGGTATTTTAGGAATTTTTTATTGCCAGTTTATCCATGTCAAATTAAGAAATTACTTCTGAACTTATTTCTTTCTTTATGCCTTTTATAAAGTAGCTGCTTTTTTCACTACACTTTTTTTGAATAACAGAAAGCAAATGGCACAACAGAAGTTTGCTTTTTATGAAAAATCACTTCCCAATAAGAAGCGTTCTTTTTTTGAAATGAAGTATTACAATGATGTAACAACACCCAAAATTTATCGTTTAACTGATCAGGAAATGAAGTTATTTGATAAATTAGAGGCAGTTACAACCAGCTAGATTTTGTTAGAATACTTCAGTTGTCTATATGGCTACAATAGCGCAATTAATTAGAAAACCACGCCAAAAAAAGAAGGTTAAATCAAAGTCACCTGCACTCCATTATAACCTCAACCTTTTAAACAAAAAAACTACCAATGTTTACTCACCACTAAAGCGTGGTGTTTGCACCAGGGTTGGCACCATGACCCCCAGAAAACCTAATTCTGCACTAAGAAAGTATGCTAAGGTTAGACTTACAAATGGCTTTGAAGTACTTGCTTATATCCCAGGAGAAGGTCATAACCTACAAGAACACAGTGTTACTTTATTAAGGGGGGGTAGAGTAAAAGATCTCCCTGGAGTTAGATACCATATTGTTCGTGGTACTTTAGATACAGTTGGTGTTGACAAAAGAAGACAACAACGTTCTGCATATGGCGCTAAAAAACCAAAACCAAAATCTTAACTTGATCAGTTAAATAATGAGAAAAAATCGTGCTTTAAAAAGAACTGTTTTACCCGATCCTGTTTTTAACAACACACTGGTTACAAGGATTATTAATGTCATCATGAAAGATGGCAAGAAGGGTTTAGCACAACGCATCTTGTATGGTGCTTTTGAGATCATTGAAAAACGCACCAACCAACAACCTTTAACTGTCTTTGAAAAAGCAGTTGATAATGTTATGCCCCGCTTAGAGTTAAAAGTGAGAAGAATTGCTGGTTCTAACTACCAAGTACCAACTGAAGTTCCCCCTGACAGAAGGATTGCTTTAGCACTAAGATGGATTGTGATCTTTGCTAACAAAAGAAATGAAAAAACAATGCTTGAACGTGTTGCTAATGAAATTATTGATGCTTTTAATAACACGGGTGCTAGTGTTAAAAAGAAGGATGATACTCACAAGATGGCAGAAGCTAACAAAGCCTTTGCCCACATGCGTTGGTAGTTATTTATTATGTCAAGAACAGTTGATTTAAAAAACTTCCGTAACTTTGGCATTATGGCCCATATTGATGCTGGGAAAACCACCACATCAGAACGTATTTTGTTCCATTCAGGTAGAATTCACAAGATTGGTGAAACCCATGATGGTGAATCAGTGATGGACTGGATGGAACAAGAAAAAGAAAGGGGTATTACTATCACCTCTGCAGCCACTTCAGTGAGCTGAAAAAACTGCAGCTTAAACTTGATTGACACTCCTGGCCATGTTGACTTTACAGTTGAAGTGGAGCGTAGCTTAAGGGTTTTGGATGGAGCAATTGCGGTATTGGATGCTCAAATGGGAGTAGAACCACAAACTGAAACAGTATGAAGACAAGCTTCACGCTATGAAGTACCACGGGTAATCTTTGTTAATAAGATGGATAAAACCGGTGCTAACTTTGAGCGCTCTGTTTTATCAATTCAACAACGCTTGGGAGTGAAAGCTGTTCCTATTCAATTTCCCATAGGTGCTGAAAATGATTTCAATGGCATCATTGATATCATCACTAAAAAAGCTTATTTTTTTGATGGTAATAAAGAGGAAAATGCTATTGAAAAACCAATTCCTGAACAGTATGTTGATCAAGTTGAAAAACTTTACAACAACTTAGTTGAAGAAGTTGCTAGTTTAGATGATCAACTCATGGCTGATTATCTAGATGGTAAACCAATTGAAATTGATGCAATTAAAAATGCAATTAGAAACGGGGTAATTCACTGTAAGTTTTTCCCGGTATTGTGTGGTTCAGCATTTAAAAACAAGGGAATTAAACTCTTACTTGATGCAGTGGTTGATTTTCTCCCTTCACCTGTTGATGTCCCACCTGCTAAAGCAATTGATGCAAACAACAAAGAGATATCTATTAAAGCTAGTGATGATGCTAACTTTATTGGCTTAGCATTTAAAGTTGCTACTGATCCTTTTGTTGGTAGATTAACTTTTATTAGGGTTTATGCAGGGGTTTTAAAATCTGGTTCTTATGTTAAGAATGTTAGAAAAAACAAAAAGGAAAGGGTATCACGTTTAGTGAAAATGCACGCACAAAATCGCAATGAAATTGATGAAATTAGAGCAGGGGATATCTGTGCAGTAATTGGCTTGAAAGATACTACTACTGGAGAAACTTTAACTGATGATAAGCTTGATGTGCAACTAGAAGCAATGCAATTTGCTGAACCAGTGATCTCTTTAGCAGTAGAACCTAAAACTAAAGCAGATCAGGAAAAGATGTCAATTGCTTTATCAAAACTAGCAGAAGAAGATCCTACTTTTAAAACCTTTAGTGATCCTGAAACAGGGCAAACTATTATTGCTGGAATGGGTGAGTTACACCTTGATATCTTAGTTGATAGGATGAAACGTGAATTTAAGGTAGAAGTTAACATTGGTGCACCTCAAGTTAGCTTTCGTGAAACCTTTAAATCAACTAGTGAAGTTGAGGGTAAATACATCAAACAATCAGGTGGTAGAGGTCAATATGGACATGTTAAAATCCGTTTTGAACCTAATAAAGATAAGGGCTTTGAATTTGTTGATAAGATTGTGGGCGGAAGGATTCCAAGGGAATATATTAAACCAGTTCAAGCTGGTCTTGAAAATGCAATGAATTCAGGTCCTTTAGCAGGTTACCCAATGATTGATATTAAAGCTACCTTATTTGATGGTTCTTTCCATGAAGTTGACTCAAGTGAAATGGCTTTTAAAATTGCTGCATCCTTAGCTTTAAAAGAAGCAGGTAAACAATGTAACCCAGTTTTACTTGAACCTATTATGGCAATAGAAGTTACTGTACCTGAACAGTACTTTGGGGATACAATGGGTGATATCAGTTCAAGAAGAGGGATCATTGAAGGTACTGAACAACGTGATAATGTTCAACTAATAAAAGCAAAAGTACCTTTAAAAGAGATGTTTGGTTATGCCACTGATTTACGCTCTTTTTCCCAAGGTAGGGGTAATTATGTAATGCAATTTAGCCATTATGCTGAAACTCCTAAAAGCGTTGTTAATGAGATAATTGCTAATAAAAAATAGATACTTAATAAAAATATAACTTTACTCAATGAGTTTTTCCAAAAAGTTTTTATGCACTACAATATCATTCTTTTAGTTGATGGTACGCTTAGTTTAGAACAAGCTAACCAAGTTGAACAAAAACACCAAAAATTGCTTGAAAAGGCAACTGAATTTAAAAGTGAATACTTAGGTTTAAAAGAGTTGGCTTACCCCATTAAAAAGCAACTTTCTGCTCACTATTACAGATGGAGTTTTCATGGTGAAAGCAATTGTACTAAGGAGTTTAAAAGAGCTGCTAACATCAATAAGCAGATAATAAGAGAGTTAATTATTAACAGAGAAAAAGACTATGGTTATTTAGGTTCAGTTAACCCTAAAAAACAACAACTGTCTTTGCAGAAGCTAACCAAGTATAATGAGATTATTGCTAGTGAAAATAATCCTGATAACCCAGATGCGCCTGTCACTTCTGGTCTAGCTTCTGTTAAACCACGGCTATCAAGAGTTGAAAAACAAAAGGAACGTGAACTTGAAAAGTGAACGGTTGTTCACCAATCAGGTAACTTTGATACTGTACAGATCAATCCTTATCGTCCTAGGATAAAACGCTTTTTACAAAACAACCAACAAACCTCCCAAGCTAATAATAACCAACCTCGTTTTCAAAATCAATTTAAAAAAGGAGCAAAACCTTAGATGAACCGGGTCTTCTTGTTTGGTAAACTCAGTTTTACTCCCAACCGTTTACAGACAAAAAATGGTACGTTAGGAGCTACTTTTTCCATGGAATGTCTTGATTCCAGTGGTTTTAATAATGCCAAATCATTCATTAGAGTAACTGCTTGAGGTAAAGTTGCTAGTTTTATTGTTGCTCAAAATCCTGGGGTGATGCTTTTTGTAGAAGGAAGATTAACTACATATAAAATTACTAACAGTGAAAATAAAAACACCTATGCTTTACAAGTAACTGCTGATAAGATCTTTCATCCTGATGAAAAAACTACCAATGAAGAACCTATTAAATCAACTGTAGTTGATTCACCCTTTATGAATCCCAAAGCAAGTGTTACAGAAGCTGAGTTTGAACAAGCATTCCCCCATCAAGATGAAACTGATTTTAACAACATTACCCCTATATTTGAAAATGATGTCCAACTAGAGGAGGAAAGTGATGATTAATAAAGAACAGGATTTAAACCAATTAGAAACCAACCAAGAACAGAGTGTTGAACAAAACCAAACTGATGAAAAGCGCAAGCCAAAACCAAACTTTAAAAGAGCAAAAAAATATTGTCGATTTTGCGCCATAGGTCAACTAAGGATTGATTTTATTGATGATTTGGAAGCAATCAAACGCTTTCTCAGTCCCTATGCAAAGATTAATCCTAGAAGAATTACAGGTAATTGCAACATGCACCAACGTCATGTAGCTAATGCTCTAAAACGAGCACGTTACCTAGCTTTAGTGCCATTTATTAAAGATTAAATATGAAGATAATTTTGAAGCAAGATGTTGCTAAATTAGGCAAGCGGTTTGATGTTGTTGAAGTTAAAGATGGGTTTGCTATCCATTTTTTATTTCCCAAAAAACTAGCTGCACCTTTAACAAAGAAAGCAATTGCTAACCGTGATTTGTTTTTAAAACAACAACAAGAACAATACCAAAAAAATCGTGCCTTAGCTGAAAAATTGAAACTAGTAATTGAACAAACACCATTAACTTTTCAACTCAAACAACACGATGGCAAGCCATATGGTTCAATCATCACCAAACAAATAATTAATTTAGCAAAACAACAAAGACTTGATTTACAGCGCTTTATGTTTAAAGATAATGTGCGCTTACAGTTTGGTGAACACAAACTAATTTTGCACCTTTTTGAGGAGATAACTGCAACTTTAACTGTTATAGTGAACCCTGAAAATGGGACAACAAACTAGTTTTAAATATGCAAATGATAGCAACATTGAACGTGCTGAAAGACGTTTGATGCAAGCAGTTGCTCAAAACAGTGAGGGCATTGATCTAATTTTCAATAAACTTGAACCAATTGATTTTTTTGCAACCCCTTTCAAACTCATTTTTCAAACTGCAAAAGAAAACTACCAATTAAATAACCCTATTATTGGTTCTGGTTTACTAGAAGCGGTTAAGTTTAAACTTGATGCTAATGATCAATCCACTAAAAGTGAACTTGAACTTTTATTCACAAAGATCTTATTAATCCGTTTACCACCTAACCAAACAGAGATTAAAACACTGGTTGATGTTGTTAAAAAAGCTTCTATTTTTCGCAGGTTACAACAGTTTGCTAAGCGTGTTTACAACGAGGAATTTAAGTTAAAAGAAGATCGTTTTGAAGGCTATTTACAAGCTATTCAAGATGATTTTGTCAAGATTATCCACAGTGCTTTTAGTAACATCTTTGCTTTTAGCTATGATGAGATTGCCAATCAAGAGGAAGCATTAATTAAAAAGGTTCACCGTGGGGAATTGATCATCAGTGGACTTTCAAGTGGATTTTTAAAATTAGATCAACTTACATCAGGTTGAAAACCAGGAGAGTTAATAGTAATAGCAGCTCGCCCAGGTAGAGGTAAAACTGCCCTTTTGATTAATTTTATGGCTAGTGCAGCTAAACAAATTGATCCTAAAACTGATGTGGTCCTCTTCTTTAGTTTAGAGATGCGTAACCGGGAAATTTACCAAAGGCACTTAATGCATGAAAGTCAAACTAGTTACACACTAACCAACCGACAAAGGATTAATAATGTCTTTGAAGAGTTAATGGAAGCATCTTCAAGGATCAAAAACTTACCTATTAAACTCTTTGATTACAGTAGTTTAACACTCCAAGAGATCAGAAACCAAATTACTGAAGTGAGTAAAACCAGTAATGTTAGGTTAGTAATTATTGACTATTTACAACTTGTTAATGCTTTAAAAAATAACTATGGTTTGACAAGACAACAAGAAGTGACAATGATCTCTCAATCACTTAAAGCATTTGCTAAGGAGTTTAATACCCCTATTATTGCTGCAGCTCAACTTTCTAGAAGGATTGAAGAAAGGAAAGATTCCAGACCAATTCTTTCTGATTTAAGAGAATCAGGTTCAATTGAACAGGATGCGGATATGGTTTTATTTATCCATAGAACTAATGATGATAAAAAAGAACAGGAAGAGGAGAACACAAACTTGTTTGAAGTGGAGCTTATCTTAGAAAAGAACAGAAATGGTCCCAATGGCAAAGTTAAACTAAACTTTCGCAGTGACACTTCTTCTTTTATTAGTCAATATTCCCCTAGTTTTGATGACCAATACAGTTAATTTATGAAACTGAAGTTTTACAAACTACCTTTAATTACTACAGCATTTAGTTTTGTATTTTTAACTGCTTGTTCAACACCCCAATCTTCCTTTTTTCTCCCTGCACAAACAACTATTAGTACTTTAAAAATTAATGGTATGGAAAACAAAACAGGTTATTTTCTTGAAACGCAGCGTTCTCGTGGTAGTTACAATCCCACCGCTTCACTTACTATGATTAAGTTAGGAGATGAAAAAGAGTTTAAAAACGTAGATACAACCAAGCAAGATGAAGTTTTGTTTGCCAACATTTATGGTGGAATCTCTTCATTGCTTAACTTCAGAATTATCCAACCGATGTTAACCTACTGAAACTTAGTTAACAACTCATTAAGTCAGATTGGTAGTACTAATGACTTAATTACATTCAAAGATAGTGGATATAAAGATCAACTTGCAAAAGCGCTTGCTAACAATCTCATAGTTGCTGATGAAGGTAATAATAACTTTTGGTTTGGTTTAAAAGCCCTAAAGTTTGATACGGTTAAACTCCAAGCTAATAATACTGCTACTAGTTCAACAAGAGCTTCAACTACTCAAAATACTAATAATAAGATTGATGCACGTGAAAAAATCACCATTAATGGGAATGGTGGAACAAATAATGATCAAAATGCTACTGTTCAAAAGTTGATAGGTATTGAAAATATTGAAGTTGAGTTTAGCTTTGTTAAAACTGGTTTTAATGGAAATGAGATTAAGTTTGAAGATTATGTAACAGATTCTTCCCCCACAACATCTTTATTAAAGCAAGTTTGAAAAAGTAAGTGAAATACCGAGTTGACCCACACCAGCTTTAAATTCAAACTCAATTCTTTCAATGTTTTGTTAACTTATCAATTAGAAGCAAACCAAAAAAGCCAATATCTTCCCAATGGCTTTAGCTTTCTTTTTCCATCTAATTTAGAAGGAAAGATAGATAGTTCAAAAAGCTATTGAAACAATCTTGTTGATTTTTCAAAAAGAACCACAAATGAAGAGAACACAATGCTACTAACTGATTTGCAAAAGAAACAAGATCAAGTGAATCGGTTTGTTGGTTTTATTGGTCAAAACCACTTTACTTTAAGTGCTAACAGCATTAATGAAAAGCAGTTTAACGATGCTAGTACTGCTGATTTTTTTAAAGCAATCTTTCAAAAAGTGAGTATCAATGAATAACAATTTTAGCCATTCAGTTTTGAAAAGAAAAGTTTAAATTAATGGTAAAATTATTCATTAAACTTTCCTTGTTAGTTGTAGTTTTCAATACTGTTTGTATTTCGAAGTTCATTTTAAAAAATTCGTAGTTCTAGATCTTTAAAATATGTTTATTTAATTGTCTTTTTTAATTTCATAAATTGTTTGAAAACACAAAAAAATATCTTTAAGGTCAAGGCCTTACTACTTTCACTTTTTTCTGCTGCTGTTACCATCACTATTTTTGCTTTACCTATCTTTGCAAACAATGGTTCTAAAACTGATTTAGGTTTGCTTTCTAAAAATAGTGCTGATTTTTTAGGTAGTTCAAAAAGAAGTCTTGCTGGCTTTGATACTCCTTTTAGTCCAGATAACCTCCAGTATTTAGAAAAAGAAACTGACTATGATCAGAACTTTAAAAATTTTACTGAAAAGTTTAAAGATGAAAAAATAACTAACAATCAACTTGATATTGTTGATATCTATAACCTATTCAGTGGTTTTCACAAGAGTGTCAAAAACACAGTTGACTTAATGAGCCAACTGCAAAAGCAAGTTGAAGCTGCCAATGCTATCTTCCCAGTTGATGATGCTTTTGTTAAATTACCTAAAGTACCTACTGAATTATTTAAGTTAGTTGATGATAATGTCTTTCCTAAGTTAAATCCTAAGGGCTTAAATATCTCTGATAATATTGCTGCACTTTTTGAAAGATATAACCTTAAATCGATTGAACTTAAGAACTTTGACTTAGCTTTCTTGAGAAAAGCTGATGTAATTATCAAAGACAAGGTTCGATATAACTTTGAGATGCAAATGCAGTTTCAAACTGTTTATGTTGGTGGGGGTAATACCGTTATTAACTTAGACTTTACTTTAAAAGCCCAAACCGCTAACTTTGCTAACCTCCAGGATTTACAAAACAGTTTTGTTAAAGTTGGTAATGATCTTTCCACCCAACTCTTTTGGATCCCAACTGTTAATAAATTAACTGATAATGCAGGTAATGATCTTACCCATATTGCTAAAACTGTGATTGGTGAATCGTTTTTCCAAACCAATGTTAACTTAGCTAAATCAGTTATTGAATATGATAAGGTTCAACCATTGGTTAAACAAGCTTTTGAAGAGCGAGTTTTAACTCCTTTCAAAAAGGAAAGAGAAGCTGCTAAAAAAGCTTATGAAGAAGAACAACGTCGCTTGGAAGAGGAACGTAAGCGTCAACTAGAAGAGCTAAGGAGAAGAGAAGCTGAGGAGAAAAGAAAAGCTGAAGAAGCAAAACGAAATCAAGAAAAAGCACGCAGAGAAAGAGAAGCTTATGAAAAATCATTTAACTCCTTTAAAGACTTTAAATTTTACTGGTTAACTAAAGGTAAAGATGTTACTAAAAAAGCTGATTTAATTGATGCACTTAAAACCGCTATTGCTACACCAGCATACAGAAATAGAACATTCTCTTTATTAATCAAAGGTTTTGCTAGTGGAGTTGAACGTTATTTCAACGCTAACAAGAATGATAAAGAGTTGAAAAAACTTGCATTTGGTGAAAAAGGGATCCAATTCCCTAGAGCAGATGCTGGGATAGATGGTCTTTACATGAGTAATTTTTTGAGGCATGAATTGACAAGCAAAGCAAAATTTTCACTTAACCTAAAAGATATAAAAGTTGAAAATACGGTTGAAGATACTCAATTGTATTGGAAGGATAATGGCATTCATTTAAAGCAAGCCAATCCTTATAAATTCAATCTAAATATCAAGATCAAGTACAACGGCTGGTATAATGTCCACTGGTGAAACTGGCTCCCTGCTAAAATTTTAGGGATTCCTACAGACTGAAGTGGTGAGATGAATTTAACATTTGTTGTCAACGGTGACCTATCAGAAATTGTTGATAAACATGATTACCCTGGTACTTTCTTTCAATTTACCGATAAAAATGAATTGCTATTTACCTTAGCAGTTAGAGAACAAATTAAGGTTGATAATAATCATTTTATGGGTCTGTTAAAAAGCCAAAACCTTCATGATCTTCAGCTTGCTTCTGGTGCTACAAAACCTCCTGTTGTTGATTTAGCTAGTTATTTCCACTTTGTATTATTGACTGAAAAATCTTAATCTATTAAGAAATTTTTGATCAGTTACTTTTACCTAGATAAATTTAAAGTTTATTTGTGGTAATGGATGATCTATTCCAAAGAATGGTTAGCTGTGTTCTACCGTCATGAAGAGCTTTTATTGATGAGGAAGTTAAAAAACCTTATTTTCAAGCTTTATTAGAAAAATTAAAGGCTTTAAAAGCAACAATAATTCCAAAACCAGAACTTATTTTCCGTGTTTTTAGCTTCTTTAAGCCAATTGATACAAAAGTAATTATCTTTGGTCAAGATCCCTATCCTAATCCTAATGATGCATGTGGACTTGCTTTTGCATCCAATAATTCCAAAACCCCTGCCAGCTTAAAAAGAATAATTTTACGTTTAGAAAAAGAATATCCTTCGCTTAAACAAGAAAGTAGTTGACAACAAAACTTCCTATTGAATTGAGCAGAACAGGGCGTTTTATTACTAAATGGAATTTTAACAACTACTGTATTTATACGCAACGCCCATAAAAATTGGGGTTGGGAGGAGTTTAACTGTAATTTGCTAACTTTTCTAAAAAATCAAAACATTAAACCGCTGTTGGTATTTCTGGGTGTTCAAACTAAAAACTTTGTTGTTAAGAGTATTGGTAATGTTGATGGATTTGAGCATTTATCATATCCCCATCCCTCACCACTAAGTGGTAATTTGTTTCTAACAAACCCTAACGATCTGTTTAAAACAATTAACAATTGGTTGAAACAACATAACCAAAAAATAATTAACTGAGCAGTTGTTAAAAATGCTAGTTTTGACCAATTAAGTTAATTAAAAACAAAGCTGTAGTTGACACTTGTTTATTGATTAGAAAAACAACAACCTTGTAAAATTATTTAACTAAGTGATTATTACTTAATTGCACTTTAGATGGGAGAGATTAAAAATACTGCACCAACAAGCGATATTTCCACTTCAGGATTTATCTATTTTGCAGTTGTTTTTCTAATCATTATAGTCTATCTTTTTTTTAAAAACATTCTCTTTTTGTTCTTCTTTAAAAGATATCCTAAAAACACACCTAAAATTGGGGTTAGCAATATTACTACTATTGCTATGATTATTGCTGTTGCTGTATCTGTTGTTTTGGTCTTAATGGCTTTAGCAGGGGGGTTAACAGCAGCGCTGTTCCGTGGTTATCCTGGGTTCCGTGTTACCTTAGAGTTAATTCTAGTGAAGATTTCAGGACTTTTATTTGGTCCTATAATTGGTATTTTTTCAGCAGCTACCATTGACTTTTTAACTGTTATTTTTTCAGGTGGGGTGTTTAATATTGGTTATGTTTTAGGAGCAATCCTAACGGGAATGATTGCTGGAATTTTACGTGAAGTTTTAATTTCAACTTCTTTTTTAAATAACAAAACTTTAAGTGATTTTGCCTATCTTGTTTTATCAGTGGGGATGGTATTTGCCAGTTTTTTAGTAACCCAGTTTTTTGTTATCTCAGTTACCCAAAACTTATCAGCATTTCAAAGTAATGATCAAATTGTTTTACGTTTTAACGCCTCACCTTTAAATTTCAGTATCTCATTGCAAAGATATGTTCAAATTATTTTCTATTTTGCGATGGTTGTAATCATCACAATGGTAGTACTTTACTTTGTTTGAATAATCAAGCAAAAGCACTTTAACTATGCTTATTCCAAGTTTTTCTTTCGTAGATATAAACATGCTAATCACCAATTTACCTTATTTGTGTTAACAAAAGAAAACTGGTTTTATCTAATTCTGAATGTAATTACATTAGCAACCACCAGTTTGCTAATGATTAACATTGCATTTATTCCTATCTTTGATACCCAAACAACTGGACAAACTTATGATTTTTGATTATTAGTTAGATTATTGTTTGCTCCTTTGATCTTCTTACTTGATATTATTGTTATCTATCCAATCTTGTTATTGTTAACCCCAATCATGTTAAAAGGGTTTAAAACAGTAGCATCAGAAACCCAAACAAAAGGAATCAAAAAGAGTTTTTCAGATATGCAAAGCTTAATTATGCCCAATGTTATTAGTCACAAAAAACAGCAGTTAATTAGAAAAGAGATGCAACAGTTAGCAAAAACAATCAGAATTGATTTATCAGACAAAGAAGTGGATGCACTTGTTGAAGAGTTTAAAGAGATCACAAAGAGTTTTAATAAGGTAACTAAAATTGATACCACTAATGTTCAACCGATGTACGCTCCATTTGAATTTAGTCCAACCCCACTAAGAAAAGATAAACCAGTAGTTGATAAACACGCTAAGCAACTACTTAATAACTGTTGTGAAGTTAAAACAGGTTTTGTAAAGGTATAGTTGTGCGATCAAATATTTTAAGTCTCAGGGCGATACTTGATAAAAAACCTAGTGCCATTAACGATGTTTTAACATCAATTAATGCAAAGATAGAACTGAATAAATCAAGTAATTTTTTATTGAAAAATACTGTTGAAATTTATTCAAAAAAAATTAATAAAAGTGATGAAAAGATTCTGCTAAATAACATCCCTTATGTTTTGAAAGATAACATCGCTACTAAAGATATTGTCACCACTGGTGGTTCTTTGTTTTTAAAAAACTATCTTCCCCCTTTTTCAGCAACTGTGTTTGAACTGTTAGAAATGAATGGCGCGTTGCTTGTTGGTAAAGCTAATATGGATGAATTTGGCTTAGGTGGAACAGGTAGTTATTCTGCTTTTGGTGTTGTTCATCACCCTGAAAATTCCAGTTTAATTGCAGGTGGTTCTTCCTCAGGTTCAGCTTACGCAGTTGCTAAAGACATTGTTCCTTTTTCCATTGCAACTGACACTGGTGATTCGATTAGAAGACCTGCTAGTATCTGTAATGTTGTTGGCTTTAAACCAACTTATGGTTTGATATCACGTAATGGGGTATTTCCATATGCCCCAAGTATGGACCATGTGGGGATATTTGCTAAGTTTGTTAGTGATATTGCCATTGTTAGTGATGTTGTTATTAAACATGATAAAACTGATTTTTCTTCCCAAAAATCACCTGATGAAAACCAGTTTTTCAATGAGTTGGCCATTCCCTTTACAAGATCAATTCGCTTTGGTTATTTAAAACCACTAGAAAAACTGTTTAACAAACACCTCCAAAAAAAATGAAATAATCTCAAAAAAACCTTAGAACAAAAAAACTACCAGTTGATTCCACTTGATTTTGATGTGGAACTTCTCAAAGTAATTGATTCTATTTACAAAATAATTAGTTATAGCGAAGCAGTTAGTTGTTATAGTAATTTAACTGGCATTGTCTTTGGTCAAAAGGTGTTTGAACCTAATTCACCAAGTAATTTTGATCAAACTATTACCAGAAACAGAGATCAGTTTTTAGGTAAACAACTAAAAAGAAGATTTGTAATAGGGGCATTTGCAACTGATGAGAAGAATTTTGAAAAGTACTTTGAAAAAGCTCAGAAAATAAGAAGAATCTTAGTGGATAACTTTCTGAATCTCTTTAGTGATGTTGATTTTGTATTATCACCAAGCGCTTCTTGTTTTGCTAGTACCATTGAAGATATTCAAGCTAATAAGCCATATACAAACATTATTGATGACTTTTTACAATTAGCTAATTTTGCTGGTAGCCCTTCTATAACTATTCCTTGGTTAGTTCAAACAAAAGACCAAACAATTGGTTTAAGTATTAGTGCTAACTGTTTTGAAGATAAAAAACTCTTACAAATTGCTTATTGATTTGAACAACTTTTTGATTTAAACCATGATTAATTTTGAAGCGATTATTGGAATTGAAGTCCATGTAGTTTTAAATACTGCTAGCAAGATGTTTTCAAAAGCACCTAACCGCGTTGATAATCAAAAAATCAATCATTTTATTGACCCAATAGATTTAGGTTTACCAGGCACTTTGCCTCAAGTTAATGAGTTAGCAGTTTACAAGGCATTATTATTAGCTGATGCATTAAAAATGAAGACAGTAACAAATAAACTTGTTTTTGATCGAAAGCACTATTTTTATCAGGACTTACCTAAGGGTTTTCAAATCACCCAACAAAACTATCCTTTTGCTAAAAATGGTGTAGTTACCATTAATGTTGATGCTATTGAAAAACCAATTTATATTGACCGGTTTCATTTGGAGGAAGATACTGCCAAACAACACTTTAACCATGACCAAATTCTGCTTGATTTTAATAGGTGTGGTGCACCTTTAATTGAAGTTGTTACTCTTCCTGTTATCAACACTGCAAAAGAAGCGAAAGCCTACCTACAGAAGTTGAGACAAATTCTGATTGTTAACAATATCTCCAATGCCAAATTGGAAGATGGTTCAATGCGGAGCGATTGCAATGTTTCAGTACGTTTAAAAGGTCAAAGGCAACTAGGAACTAAAATTGAAATTAAAAACATCAACTCACTTAATAATGTTGAAAAAGCGATTGATCTTGAGATTAACCGCCAAGTTAAAGCACTTATTAATGGTGAAACCTTGAGTCAAGCAACTTTAAGCTTTGATGATAAAACCAACAACAATGTTTTTATGAGAAAAAAAGACAATACGATTGACTATAGGTACTTTATTGAACCTAACATCATGACTAGTAATATTGATGATTTATTATTAGAAAAACCTGTTGCTTTTCAGTTAGAACAGTTTCAAAAAGAACTAATTGATAGTAATGTCAATCCTCAATTAGTCCAATTAGTAGTTGATGATGCAACTATCTTTAGTGCTTTTCAAACTATTAATAGTGTGATTAAAAACCCCCAAGAAACCATCAGGTGGTTATGTATTGAACTAATAGGTCAACTCAATAAAACCAACAGTTCATTAACAGCTAATTTAATTCAAGATCTAATTACCCTAATTGAAATGCTAAAAGCAGCAAAGGTTAACCAAAAACAAGCTAAGCAGTTAATTACTTTAATGATTGAAACTAAAAAAGATCCAAAATCGCTAGCTAAGCTCCATAATTTAGAGCAAATTACTGATCCAAAAGAGTTACAAAAGATCATTAAAAAAATCTTTCAGGAAAATGAAAAAGAGATCCTGAAAAACATTGATAGAATTGAACGGATTCAAAAGTTAATTATTGGTCAAGTTATGCATAAGACCAATAATCGAGCAAACCCCCAACAAGTTTTTATAATTGTTGAAAATATGCTTCATGAAGTTCGGGAAAGAGATAGCTAAAAAAAATCAAATTATTTATCGCTATATAATCCTTAAAATTCAAAGCTTTGAATGACCTGCTAACACCCGTATCTTCTCAGAACGCCAACTAGAAATCCGTTTCAACTCCTCACGGAGTCAAATTCGCTCTGTTCTCGCTACGCTTTTAAACAAAAATATAATCCGCTACACTAAAAATACCCCTGGTTATTTTGTGTGTAAAGATGTAGGCTTTAGTTTTTTTCATAAAACCCAGGATAATCTGAAGGTGAAATATGCTAAACTTTCAACTTTGATTAAAAAGCTGTTATCACAGGATGATGCTAGTGTTTTTGCCAATATTGACAGTACTGTGCACCTTGATAAATTTAAGGGAATTGAAGCTAAGTTTTTTGATGAGAATAAAAAACACTTTTTAAACGTTTGTTTTTTTGCTAAAGATGATATTTTAAACATCCTTGATGAAAACAACCTCCAGCAACAGTTCTTCAGGGAGTTTGCTTACAATGGGATTGCTATTGAAAAACGTCATTGTGTTACCTCTGTTGACAAGGAAAGTGGGTGCTTGGTGATGTATGACATGTATTATGATGACAATGATAATTTTGTAGTTGCTAGCAAAAGTAACTTCCTTAACCCTGAATTGAAAGTAATCAATGCTTAAAGTGAATGCTGATTTTTTAACTAAAGATCAAGTTATCTATGATTTAGTGATAGTAGGTGCTGGCCCTGCTGGGATTGCTAGTGCCATTTATGGTAAACGTGCTAACTTAAATTTAGCAATTATTGAAGGAAACACTCCAGGAGGGAAGATAGTAAAAACTAACATTGTGGAAAACTATCCTGGTTTTAAAACCATAACTGGTCCTGAATTAGGTCTTGAGATGTACAACCACTTGTTAGCATTTGAACCAGTTGTTTTTTATAACAACTTAATCAAAATTGATCATCTTAACGATACATTCATCTTGTATTTAGATAACAAAACGACAGTTTTTAGCAAAACTGTTATCTATGCAACAGGGATGGAAGAGAGAAAACTTGGCATTGAAAAGGAAGATTATTTTTATGGTAAAGGGATTAGTTATTGTGCTATTTGTGATGCGGCTCTTTACAAAGGTAAAACAGTTGGTGTTGTAGGAGGAGGTAATTCTGCAATACAGGAAGCAATTTATCTTTCAAGTATTGCTAAAACAGTTCACCTTATTCACAGACGTGAAGTGTTTAGAAGTGATGCATTACTAGTTGAAAAATTAAAAAAAATTAGTAATGTAGTTTTTCATTTAAATGCTACTGTAAAACAGTTAATAGGTCAAGAAAAGCTCCAAACTGTTAAATTGGCAAGCACAGTTGATAAATCAGAAAGTGAAATTGCAATTGATTGTCTCTTTCCTTACATAGGCTTTGAAAGTAATAACAAGCCAGTTTTAGATCTTAAGCTTAATTTAGATCAAAATGGTTTTATTTTAGGAGATGAAAATATGCAAACTAACATTAAGGGTTTTTATGTTGCTGGGGATTGTAGAAGTAAATCATTCCGGCAAATTGCCACTGCAATTAGTGATGGGGTAACAGCTGTTTTAAAGGTTAGGGATGACATTTAGTACACAGATTAAAGCTGAACTGGTCCAAAACAAATTAATAGATAAACACTGAAATGTCTTTTTAGCAGGTTTTTTTCAAAACAACTTAAAGCTACTTTACAACCGTAACTGAAGTTTTAAAGTGCAATCTGAAGCATTAAAGGAACAATTTGTTCAAAATCTTAAGTTTGACTTTAAGACAAAAGCTAGTAAGAAATACTTTCTTTTTGAGTTTAATGCAGATATTAACGTAATTAACACTCTTTTAAAACTTGATGTGACAACTAGTGAATTGGTAGTTAAACAAGTTTATCTCATTGCTGCTTTTTTAAGTGGAGGTAGTGTTAGTGATTTAATAAACTCCAATAACTTTCACTTGCAAATCAGTTCCAACAATGAGTTTCAAATTCAACAACTTTTAAAATTGTTTAGTTTTTTTAAAAAAACAGTTAAACAAAACCAGTTAGTTGTTTATCTTAAAAGTTATGAAAAGATCTGTAATTTTTTAAAACTGATTCAAGCCTTTGATGGTTATCTTGCTTTTGAAAATAAGCAACTAGAGAAAAGTTTTACTTTAAACCAGTTAAGAAAAAGTAATTTGGAAGTTGCTAACTTAATGAAGACAATCAGATCTAATAATCAAACTAATCAACTCCAACTAAAATCATTTATTAAAAGTAGTAGTTTTGCAAAAAGACCGCTTAATTTTCAGCGTTATTGCTTAATTAAAAGTGATCATCCTGATTGATCTTTAGAACAGATAGCAAACTTTTTTTTCACAAAATATAACATAAAAATTAGCCGCAGTGGAATCCAACATTTTAGTGTTAATCTAAAAAAACTATGCCAGTAGTTTAAAATTAGTTCACAAGCAATGCATCCAATCCAAATAGTAATGTTCATTATGGCTGTTATCTGTTTAATTATTGGACTTTTGCTTTCTAACCATGGTTCTACTGGAGGATTAGCTTCTCTATCAGGTCAGGACTTGGAGATCTTTCGTAAAACCAAAGATAGGGGTTTTGTAAAGATCTTACAGATTATCATGTTTATCTTAGTAGTTTTATTTTTAATTCTTGGGTTGATATTTAGTTTTGCACCAAGATAACAATGAAGGTTTTAACTGAACTTCAAAAGCAGATATTTACCATTGTCAAAAAGGAAAATGGTAAACCTATTCCCCCTGGAATAGTGGTAAGAATGATGGAAAATAGTCCTAATTTTCCAGGTAAACACCTCATCTATCGGGCCATTGATGATCTGCTTGATTGAGCCATCTTAAGGAAAGCTGGTGGGGTTACAAATCAGCTATTAGTTAACTATGAACCTGCTGAGCCTTTACTTGATAAAAAACTACAAGGGATTTTAACCTTAGGAAATAAGAATAGTGGTTTTATCCGCTCTTTGGATGATGATAAAACTGTGTATTATGTCCATTACTCTAATTTAACTGGAGCTTTAGATGGGGATCTTGTGGAGTTTTGTAAATTAGATAAGCCACAATTTGGTGATAAGTTTGATGCTGCAGTTATTACTATTCTAAAAAGAGCAAGAATCTTGTATGCAGGTAATTTTTTAGTAGATCAAAATGAGTTTGCCTTGGAATACAAAATTGTTGCTGATAACCCTAGATTTTATTTAACTACGATTGTAAATCCTGATTCTATCCCAAATAACTTAGCATCTAACACCAAGATAGCTTTTCAAATTGATGAGTATGATCCTGATAACAACTTATGTAAGGTTTCTGTACAACAAGTTTTGGGTAACAATGATGATCCGCTAATTAATATAAAAGCAATCATGTTGGACAATTCCATTGTCTTTGAAACTAACGATGTAGTTGAACAGCATGCTAACAAGTTAAGTTTTGATACTGAAGAACAACATAAAGCTTACCGTCAGGATTTAACTGATTTAGCTTTTGTGACTGTTGATCCTACAACATCAAAAGACCTTGATGATGCTATTTATGTCAAAACAATACCAACAGGTTTTGTGCTTTATGTAGCTATTGCTGATGTTGCACACTATGTTAATAGAAATAGTGAAATAGACATTGAAGCAAAACACAAAACAAGCTCAATCTATCTACCTGGTCATTATGTTGTGCCCATGCTACCTGAGCAATTGTCAAATCAGCTCTGTTCTTTAAATCCAGCACAAAAACGTTATGTTGTTGTTTGTGAGATTAGTTTTGATAATCAGGGAAGGATTAAAACAAACAAGCTTTACCCAGCAACAATTATCTCCAAAAATCGCTTTAGCTATGATCAGGTTAACCAGTGGTTAAATAATAAATCAGAATTAAACTGTGATGAAACAGTTATCAACAGCTTAAAAGCAGCATTTACACTAAGTGATCTAATTCAAAAGCAACGTCAAAAACGCGGTACAATTGATCTTTCACACAAAGAAACTGAGATAGTTGTTGATGAACATTATTTTCCCATTAAGATAAATTTTTTGGTTCACGATAAAGCTGAAACCATGATTGAAAATCTCATGGTAGTGGCCAATGAGACAGTTGCTTGGGTGTTAACTAACAACAAAATTGCTTTACCATACAGAGTTCACCCAAGACCAAGCAAAAAGAAGTTACAAAGTTTGATTGAAACAGTTGGTGAGTTGAACATAACTAAACCCCAATTTAACTTAGATACTGTCACTTCAAGCCAAATAGCTAGCTGATTAAATGAAAACAAAGATAATCCTAGTTATGAGATCTTTGTAATCCTCTTATTAAGAACACTAGGCAAAGCTTTTTATAGTGTTAATCCCCTGATGCACTTCAGCATTGGTTCTAACCACTATACCCACTTTACTTCACCGATTAGAAGGTATATAGATCTAACCATTCACAGGTTGTTGTGAATGCATCTTTTTACTCCCGATCAATTCACTGATAATGAAAGAGATCAACTCAAACAAGAGTTGGAAAAAATTGCTGATACAGTTAATGATACAGAGATTAAAATTATCAATTGTGAAAGAAATGCCAATGATTATCTAACAACGCTGTTATTATCAAAACAAATTGGCAAAACCTTCAGCGGATTTATTTCAGCAATTACTAGCTTTGGAATTTTTATGAGAATGGATGAAAATAACTTTGATGGGTTAATCAAAATTACAACTATCCCTGATGATTTCTTTATTTTTGAAAAGGAAAAAATGGTATTGAAAGGAAGAAAAACTAATAAGGTTTATAAAATTGGCGATCGTTTGGAAGCTAAACTAAGTGAGATTGATTTCATCCAAAAACGTGCTATTTTAACACTCATATAATTAGCCAATGGTTGTTAATATCCTGCTTTTTATTACCCTTATTTTTTTACTTTTACTGTTTGTTTTTTTAATTGCTTTTGCATTTCTAAACAAACGGGTTAGAAATTATGTTGTACGTACTTGAACTAGTGTTTTCAGTAAGTCCAAACAAAACCTTGATAAAAAGAACTTTTTTGACAATTTAACTTCAACTTTATTAAGACTTTCAGTTGATAAAATTGGGGCTATTATTGCCATTGAAAAACGTGATTCACTTGATCCTTATATCAACATTGGTTATCGGGTTAGTTCTGATTTTTCCCCTGAACTGTTAGTTACTATTTTTTACAACAAATCATCTCCTTTACATGATGGAGCGGTGATTGTTAGAGATTACAAAATTATTAGTGTCTCTTCTTACTTTCCAATGACAAGACAATTAATTGATGTTTCATATGGTTCAAGACACAGAAGTGCCTTGGGATTAAGTGAAAAGTCTGATGCAGTTGTTTTTATTGTTAGTGAAACAACTGGTAAAATATCAGTTGCTTTAAAAGGGGTTATTAAAACCTTGTCTTCCAACTCAGATAGGTTGCAAGATGAAATTATCCATTACTTGTCTTCAAAGTAGGATTAATCACTTTTCATTCTGGTTTTTTAAACCATGGATTCAAAGGGTTAATTCTTTGGTAAAAAAAGCGTCCCTGTAAGTGATCAAATTCATGCTGTAAACACATTCCAAATAGTCCAGTTGCTTTAATGGTAATCTCTTTTTTTTCAAACCAATCATAACCTTTAATAGTGATCCATTCACTACGAATTACATAACCTTTGTGCTGCTTTTTAACACTTAAACACCCCTCACCACTTTCTAAAAATGCTTTATTTTCACTTTGATCAATAATTTTAGGATTGATCAGTAGGCACTTTTTTTCTTTGTTTAAATCATTGAGGTGGATGTAAAACAGTTGTTTTCAATAGCCAATCTGGTTAGCAGCTATCCCAATTCCTGGAATAATGTCATATTCTTGTGCTTTACCATCATATGAAGCATCAACATATGCAATCATCTTTTTAATACAGGTTTCAATTTGCTCATCTATTGGAAAATTAACAGCTTCAGTTGGCTTGTTAATCAAAGCATTATCATCAAAAACAAGCCAAGTTTTAGTTGGTTGAAAAGTCATTGATAAAAACAGTAAAGTTAAAATTATTCTAACAATTGAAGTGAACAATCAGGGGAGAATTTTTGTCATTACTGGTCCTAGCGGTGTTGGCAAAAGCACCCTTGTTAAAGCCTTATTAGATCATTTCAAAGAACAACTGTTCTACAGTATCTCTGCAACTACAAGAAAAAAGCGCATTAGTGAAAAAGAGGGAATTGATTATTTTTTTAAAGATAAAGATGAGTTTGAAAACTTAATAAAACAAGATGCTTTCATTGAATGGGCTTGCTATAATAACCATTATTATGGAACGCTCAAGTCTCAAGCTGAACAAGCAATTAAAAGCGGAATTAATTTAATGCTTGAAATTGAGTATCAAGGTGCTTTACAGGTTAAAAGTAAATATCCTCATAACGTTGTTTTAATTTTCATTAAACCACCTTCAATGCAAGAGTTGTTAAAACGTTTAAAAAAGCGTAATGATGAAGATGAAACCACAATTAAAAAACGTTTAGAACAAGCTAAGATAGAGTTTCAACAGATTGATAATTTTAAGTATGTTGTCACTAACAAAGAGTTTGATAAAACCCTTAATGAGTTGAAATCAATCTTACTATCTGAGTTTATTTAATGCAAGCTGCTAATGATCATTTCTTTACCGGTTTATCAAAAAAAGGTCCTGTAAGAAAGGAAAATCAGGACTTTTATGGATTTAGTTTTAATCAAAATAACCTACTAATAGTTGTTTGTGATGGTCTTGGTGGTTATAAGGGTGGCAAGATTGCTAGTAACTTAGTAGGTAAACTCTTTCTTAGTTTATTTGAAGGTTTTGAATTTAACCAGTGAGATGAAACTACAGTTAAAAAGTGGTTTGAAAATACTTTAATACAAGCACGCTTTCAACTAGAAAATTGTTTTCAAACAGTTTATGAAGCACAAATCCAGTTTGCTAGGATGGCTAGCACCTTGGTCTTGGGAATTTTAACTAAAAGTGATATTTATATCTTTTGAATTGGTGATTCCAGAGCTTACTTATTATTTGAAAACCAAGCTAAGTTAGTTACTAAGGATCACAATCTTTATAACCAGTTAGTTGCTATGAATGCTGATGAGAAATTACTTTTAAGTTATTCTAATCAACTTTTAGCATTAACAAATACTATTTCCAAAGAAACAAAAAGACCATTAGTTTATGGTTTTTATAACACAAAAATTGAACAACAAGAATTTTTATTATTGTGTTCTGATGGACTTTATAACTTTGTTGAAAAAGAGCTTTTTTTTGAAATTATCACTAATTCTAAAAACCTAAAACAAGCAGTGTTTAATCTTTATAGAAAAAGTATTGAAAATGCAAGTAATGATAATATTACTGCTGCCCTTGTTAATCTGCAAAAATGGAAGCAATCTTAAAGATAGGTGATATTGTTGAAAATAAGTATCAAATTGAAAAGCTTTTAAATAGGGGTGGGATGGATTCCTATCTTTTTTTAGCAAAAAATTTGAACTTAAAAAACTATGGACCAGTTCAAAAAAAACAGTATGGACACTTAGTTTTAAAGGTGGTTCAAAAAAATCCTAAGATTAATGAAAATAATTGGAAAAAGTTCCTTGATGAAATGGTAACCACAACTAGAGTGCACCATTCTAACCTAGTGAAAAGTTTTGATGTGGTGAACCCTTTTTTGAAAATAGTTCGTGGTAATAAAACAATTGCTCTCAATCAGATAGTGATGATTGCAATGGAGTATGTTGATGGCCCTTCTTTAAGACAATTGCTTAACAGAAAAGGCTACTTTAGTGTTAGCGAAGTAGTTTATTACTTTACAAAGATAGTAAAAGCCATTGATTATCTCCACAGTTTCAAGCACCAAATTATCCACCGTGATCTTAAACCTGAAAACATCTTATTTACTAGTGATTTAACTGATATTAAACTATTAGATTTTGGCATTGCTTCAACAGTAGTTAAAGTTGCTGAAAAAACCGAAGTTTTAACTGATGAAAATTCACTGTTTGGCACAGTTAGTTATATGATTCCTGATGTTTTGGAAAGCACAGTTAATAAAGCTGGTAAAAAGGTTAGAAAACCACCCAATGCCCAGTATGATATCTACTCGTTAGGAATTATTCTATTTGAGATGTTAGTTGGTAGAGTTCCATTTAACAAGTCAATTAACCCCAACAAGGAAAGAGAAACAATCCAAAAAGCACGCAATTTTGACTTACCTTTAATGCAAGCAACTAGAAGTGATATCCCAAATAGCTTAGAAAATATTGCTTTTCGTTGTACTGCTGTGAAAAGAGAAAACAATAAGTGACTTTACAGTTCCACAAAAGAACTTTTAGAAGATTTGGCGAATTGAGAAAATGAACAAGCGATGATTAAACCTGCTAATGAAAGGGTTTTAGAAGGGCAGGTGGAAATTAGAGAAATGATGCTTGAAAAGCCCTTAGCTTGGTATTTCAAAACTTGAGCCTTATCAATCTTTACTATCGTTTTTATAGGGTTAATTATTGCTGCAATAGTTCTTTTATTAATTTTTAATGCCAGATTCTAACTTTGGGATAGTGCTTCAGTCATTAGCAAAACAATGCAAAGTATTTTGGAATAACCAGATAATAACTGCTTTTCCTCAAAAGAAGTTACAGTGAAAAAATGATTTTAAGTTGATGGTTGGTGATAGGGTTCAACTTGAAGATGGTGCGATCACTAAAGTTTTAGCTAGAAAAAATGAACTTACAAGACCAAGGGTTGCTAATGTTGATCAGATTGTTTTAATCCAATCACTAGTTCAACCTAAGATTAACTGAATTCAACTGTTGAAATTATTGGTATATTTCAATGCTAAATTAATTGATGAAATACCTATTTTAATAACAAAAACAGACCTTGATTTTGATCCAATGGAAAAACAAAAGTTAATCGATTTAAAACAGTTTAACCATCAGTTATTTTTTGTTTCTAAAAATGAACCACTACCTTCAGAATTAATCGATATTTTTAGTAAAAAACTAAGTGTTTTTACAGGTCAATCTGGTGTTGGTAAATCTAGCTTAATTAATCGTTTAGATCCTTCTTTAAAACAAAAGATTCAAGCCTTATCAGTTAATAAATTTGGTAAGAATACCACTACTAAAACAACACTTTTTTCATTTAGAGGCGGTTTTATTTGTGACACCCCTGGTTTTAATGTAATTTCTATTAAAAACCTCAAAATTTTAGCAGCCCAACACTTTGTTGGTTTTCAGAAAATGATTAGTAAGTGTCATTTTTCTAACTGTTATCATCAGTATGAAAAAGATTGCTTTGTAACCACAAGTGTTATGAAAAACAGATATCCTTCGTGATTGTATGAGAAGTATAGAAAAATGATTAATTAAAATCAACTGCAAAAGTGTTTATGAGTGATAAATTATTAACAATTGACTTAAGTCATGTTTATGGATTTGATAAAGAAATTATTTTTAAGAAATACCAAAAAAAAGTAGATCAAATTCACCAAGATTTTCTAGCTCATAAACTTGCTGATGGTCACATGACTGGGTGGTATGACCAACCTGATCAAAACCACCAATTCCTTTTAAAAACCATTAATCAAATTGACAAAAAGTTTAAAAGTTTAAAAGTAACTGACATTGTTTATGTTGGTATTGGTGGTTCTTTTACTGGTATTAAAACAGTTTTAGATTTCTTAAAACCAAAACAAAGAACAGGATTAAAAATCCACTTTGTCCCTGACCTTTCTGCTTTTCAAGCTGCAAGTGTTATTAAGGAAATTAAAAATAAATCATGGGCTCTAATTACCACTTCTAAGTCTGGTAGAACCCTAGAACCAGCACTGAATTTCCGCATTTTTAGAAACTTATTAAACAAGCGTTATGGCAACAAACACTACCAAAGAGTAGTTGTTATTACTGATGAAAAAAAGGGATTACTAACCAAAATGGCATCAAATCATGGTTACCAAAAGTTAGTTATTGATTCAAATATCGGTGGGCGTTTTTCAACTCTATCTCCTGCTGGTTTGTTACTAGCCAAACTTTTTGGTCATGATCCTAAGGCCATCTTAAAAGGAACATTACAAGCCAAAAAGGATTTGCAAACAACTTCACTTGAAAACAATTCTGCATACCTTTATGCAGTAGTTAGACATTGACTATACACAACAAAAAAATTCAAAATTGAAGTTTGCATTGCTTATCACAGTTTGTATGAATATTTGTTATTACAGCATCGACAACTTTTTGGTGAATCAGAAGGTAAGAACGATAAATCTTTATTTCCTACTTTTTCGATTTTTACTGTTGACTTACACTCAATGGGACAACTCTATCAAGAAGGGGAAAAAGTGTTTTTTGAAACAGTAATTGATGTTAAAAATCCACTTGTTAATATTAATTTACCTCCATCTGATTTTGACAATGATGATGAACTTGATTTCTTGTTAGATAAAAGCTTAAATGAGATTTCAGATGTTGCAATTGATTCAGTTATTAAAGCGCACTACCAAGCAAATGTAAGCATTATTAAATTAACTTTAAAAGAACAATCTGCATTTATGTTTGGTTATTTTTACTTTTGACTCTCTGTTGCTACAGTGATGAGTGGATCATTATTAGGGCATAATGTCTTTAATCAACCTGGCGTTGAAGTTTATAAAAAGTTAATGTTTGAAAAACTAAGAAGTGGCCACTAAGGTTGTTTTTTCACTCTTACCACTTTTAAATAGGTTTGACAAGTCACTTTTAGAAAGTTACTTTCAAGATGGATTGAGGTTAATCCATTATGATGTGATGGACCAATTTGTTCATAATACTGCTTTTAAAGGTGAATATTTGGATGAATTGAAAACAATAGGTTTTGATGTTAATGTCCATTTAATGGTGGAACAGATCATCCCTCAAATAAATTTTTATCTTTCACAACCTAATGTGAAAAGGATTTCGTTTCATGTTGAACCATTTAGTTTTGCAAAGATTAAAGAACTAATCCAACTAGTTAAAGAAAATGGTAAAGAAGTTGGTCTTGCTTTTAAATTTACAACCAATTTACAACTATACCAACCATTTTTTACAACCATCGACTTTATCACTTTAATGAGTGTTCCTCCTGGTAAAGGTGGTCAAGCTTTTAACGAAGCTGTTTTTACAAATTTAAAGATTGCTAACCATTACAACTTGAAAATTGAGATTGATGGTGGGATTAAAGTTAATAACATTGATCAAATTAAAGCCTTTGTTGATTTCATTGTAATGGGAAGTGGCTTTATAAAATTAGAGCAGTGGCAACGTCAAAAATTGTTGCAAACAATCTAATTAAACTTTATTGATGAAATCAGTTACAGTCAAGCAGTTACTACAAACCCCACGAAAATTTAATAACAAGCAGATTAAACTATCAGGTTGGGTTAAAAATAAACGTGCTAGTGCTAACATCATCTTTCTAGCAATTAGTGATGGCTCTAGTATTAATACCCTACAAGCAGTAGTAAAACAAGAAGATAACCCCCAGGTTTTCTCACTGTTACAAACTGTTAATTTAGCAAGTGCTGTTATGGTTTGAGGGGAAATTATCTTAACCCCAAAAGCTAAACAACCACTGGAGTTGAAATTAAAGCAGGTGAGTTTATTAGCACAAGCAGAGTCTGATTATCCACTGCAAAAAAAAGAACATAGTCAAGAGTTTTTTAGAAGTAATGCGCATCTAAGAGTAAGAGCAAAAACTTACTTTGCAGTGATGAAAATAAGGAGTGTTTTGTCACACGCAATCTTTGAATACTTCTTTAAAAATGATTTTATCTTAGTGCAAAGCCCTATTTTAACTAGTAATGATTGTGAGGGAGCGGGGGAAACATTTGTAATTAAAGATAGTGAAACTTTTTTTAATAAAACGACTTTTTTAACAGTAAGTGGCCAGTTTGGAGCAGAAGCTTTTGCGCAAGCATTTAAAAAGGTTTTCACCTTTGGTCCTACTTTCAGAGCTGAAAAATCCCATACTAATCGTCATCTTAGTGAGTTTTGGATGATCGAACCTGAAATTGCATTTGCTAACTTAAAAGATTTAATGCAGTTAATACAAAACCTAATTAAATTCTTAATTAAAAAAGTGATGAAAAATGCTAGTGATGAACTAAATGTTTTAGCAAAGCAATTTAGCAATGACATTATTAGCAACTTAAAGACAATCATTAGTACTAAAAAATTTCCAATCATTGAATACAGCAAAGCATTAGCGATTCTAAAGGAATCTAGTGATACAAAAAAAACTAATTTTGAACTAAACGACTTTAGTTTTGGTATTGACTTAAAAACAGAACATGAACGCTTTTTGTGCGAACAATATTTTCAAAATCAACCGCTTTTTGTTATTAACTATCCAAAGGAGTTAAAGGCATTTTACATGAAAACAAATACTGACAATAAAACTGTTGCTGCAGTTGATCTTTTATTACCAAAGATTGGTGAGATTTGTGGGGGAAGTGAAAGGGAAAGTGATTTAAACCAACTTAAGAATAGGTGTCAATCTTTAAACATTGACACAAAAAGTTTGAACTGATATCTTGATATGAGGAAATGGGGTTATTTTGCTAGTGCAGGTTTTGGTTTGGGCTTTGATAGATTATTAGCTTATATATGTGGATTGGAAAACATCAGAGATGCTATTCCCTTTCCCCGTGTACATGGCACCATTAACTTCTAAATTCGCTGCTTATAAAAAAAAGATTGCAAACTGGTTAACAGTTTACAGAATTTTTATTGCTTTACCTACTATTATTTTTATTGCTTTAGATAATCAACTAGGAGTTTTAGCTAACTTTTCTGTTGGTGCAATTAGCATTAGTTTACAGATCAGTTTATTGATTGGAGGATTTTTGTTTTTAACTGCAGTTATATCAGATTATTTAGATGGATATTTAGCAAGAAAATGGCTAGCAGTTTCTAACTTTGGTAAATTATGAGACCCCATTGCTGATAAAGTGATTATCAATGGTGTTCTTATTGCACTAGCGATTAATGGATATTTTCACTTTAGCTTATTAATTGTTTTTATAGTCCGTGATCTTGTGTTGGATGGAATGCGGATTTATGCTTATGAGAAAAAGGTGGTTATTGCTGCTAACTGACTTGGAAAATGAAAAACTATCATGCAGATGGTTGGTATTGTTTTTAGTTGTTTTGTTTGGAGTTTTAAACAAAGTGAAATAGCTTCTTTGAATAGTGGACTGTTCTTTTGATTACTAACTCAACTGCCATATTATTTAGCAGCAGTTTTTTCAATTTGGTCTTTCATTGTTTATAACATCCAAATATATCAGCAACTAAAGGCTTATAACTCCAAGTTATAATCCTTATTAGGCATTGGAATAACTTTGTTTGCCAATTTAATAGCAGAAAAACTCCAAAAGTTACAACTTAGTGTTGCAACAGCTGAATCAGTTACTGGTGGCTTATTAGCTCATTGTTTAACTTCCATTGATGGTGCTTCTAACTATTTTAATGGCGGTGTTATTGCTTACAATAACCAAGTTAAGATTAACTTACTGAATGTTCAATCCTCCACAATTGCAAACCATGGGGCAGTTTCCAGTTTCTGTGCTAGGGAGATGGCAGTTGGGGTTAAACAAAAGTTTCAAGCTGATGTGGGTATTGCTTGCAGTGGGATAGCAGGTAGTAAAGCAGTTGAAAATAAAGCAATAGGATTACTTTTTTTCTGTATTATTATAGGAAATAAGGCTTATGATTTTGAGTTTGAAATGAACCAAAATAATCGTAAGGATAACATTGAATTATTTACCAATAAGATCTTGGAATCTTTCCACTATTTGTTAACAAAGCTAGCTTAATCTAAGTTATGAAACGTACTTTAAATATAGGTATTGTTTTGTGTGAAAATTTTCTTTCAGACCAACAAAATGCTGTTGATAGTTACACCCAAGTTTATGAAGATGTTAGGATGTTTGAATTTGGGTTAAAACTCTTTCAATCATTACCTTTTAACATCCAAGAAACCCTTATATTTTGTAATGCTGAACAACATAAAATAGTTGATAAAGCTGCTAAAAAATACAAAAACACTACTGTGTTTTTTTCACGTGATACTGATGTAGCTAATGTTTATGAAGCTAAGGTATTTATCCAAGAAAAATACAAATTAACCCAAGATTACAAAAAAAGAGGTGTTAGCTCTTATTATGACAGCTGTTGCTTTATCTTAATTGAAGCGAATCGTCCATTAACTGCAATAAAGACAGTTAAAAGTGTTTATGAAAAAGCACTGATAGAAAAAGCAGCTATTGCAGTATTACCATATAATGGTACACTAATGAATGGTAACAATGATGTTGTCTTTAGCCACTTGCAAGATAAAAATAGGTTTAACTATTGAAAACAAAGCAAGGCTTATGAAGTACAATACCCCCAAGTATATACTTTAAATAAACTTAATCAGTTTTCAAAACAGCAATTTCTAAGGGCTAGAAGTATGTTGGACTTAATGAAGATTTCTAATAAATCACCCTTAAGTATTGTTGATGGTAGTGCTTATGCTTTTAGGGTTGTTACCAACCTTGATTTTGAAATTTTATTAGGTATTTTAAAAAATGGATAAAAATATTTTTCAATTAGTACAAAGTTTTGCTAAAACGCAAAATGTGCGAGCTAATTACACCTCACAAGACAAAAATATTAGTTTAGATTTTGTTAGTTTTGAAACAGTTAGTCAATCACTAACAGGTTTTCTAATTTTTAATAATTTCAATAAATTGTTACAACTAATTGAACTGATAAAACAAAAGCAAACATGATTATATGTAGATCAGCTCTGAATTGTTGATCTTTCCAATAACAAAGCCTTAACTGATGCTACTGTTTGGATTATTAAACAAGAAAAATTGCCTGTTTCAGTTGCGGCTTTTAGCAACCAACAACTCAACCAAGTTTATCGCAATTCATCCACAAGTCCTTTGTATCTTAGTTATGTCAAACCAATCGAAGTTCAACAATTTTTTACACTTTCTCCATCCATTAACAATAATCCTTTTTTAAATCAAAATCCTTTAACTGAATCACCATTTGATAACAATAATCAGCTGTTTCAAGCAACAAAGAGCGTTGAACCTTCAATGGAAACAATGGAATTTTCCCGGTTTATGGATGAATTTGATCAGATTACAAAGAACTTTTCTGATATAGAGCTTGAACCTATGCAATTCACCCAAAGTTTTGATGATTGGAGCAAAGACTAGGGTTGCAATAGTTGGCGGGATTGGTTACATAGGTAGTTGTTTTGCTAGTTTTATCAAAGAACAAAATGATAAGCTAATTGTTACTGTTATTGATAACAACAAAAATAACCATGTAATTAAACTCTTAAAAAAGATTGGAATTGAATTCTATTTTGCTGATTTACTAGATAGACATAAGCTAACTGAAGTAATTGCAGCAATTCAACCTGATGTGGTATTTCACTTTGCTGCTAAAACAAGTGTAAGTGAATCAGTACATAATCCATTGAAGTACTTTGATTGCAATGTAATTGGTACTTTAAACCTAATTAGTGCAATTAGTAACTTACAGAAGCCAATTAAATTATTTTTCGCTTCTAGTGCTGCAGTGTATGGTCAAACAACTAATAGTTACATTAGTGAAGAGATTGTAATAACTGAAACACAAGCAACCAATCCTTATGGATTGAGTAAGTTTTTAGATGAATTAATCTTAAATGCAGTTGCCAAAAATAGTCAACTACAAGTTGTTTGCTTACGCTTTTTTAATGTGGCAGGTGCAATTCTGCCATTTGGTAATTTTAATGGTAATACCACGCTTTTAATTCCTAACTTAGTAAAAGCCTTTTTAAAACAAACTCCCTTTTTTTTATATGGCAATGATTATGCAACTAAGGATGGTAGTTGCATAAGAGATTACATCCATGTTTATGATATATGTAATGCTCATTTCTTATTATGAAAGTGGTTAAATGATCATCGCCAAATTAAATTTGAAACCTTTAACTTGGGGAGTGGGATAGGAACTTCTAATTTAGAAGTTATTGATATTGCTAAAAAAGTGTTTTATCCTAGTAGATTAAATTTAGAAATTAGACCAAAAAGAAGCAGAGATCCAGCAATTTTAGTAGCAAATGTTGCTAAAGCAAAACAAACCTTTCAATTCAAAATAACGCGTAATTTGAAAGATATGATAAGTGATGAGCGTAATTTTTATGAGAATTTTTATAATGACGCTTATTAAGTGGTGTTTAATTAATGGAAAAAGTTGCCTTCAAAATGGAGCATATCTCCAAAAGTTTTGACAATGGCAAAATTAAGGCTAATGTTGATGTTAGCTTAGTTGTTTATGAAAATACTGTCCACACCATTTTGGGGGAGAATGGTGCAGGAAAATCAACCCTGACTTCGATTTTATTTGGTTTATATAAACCTGATAGTGGCAAGATCTTTATTGGTGAAAAGCAAGTAAATTTTAAATCTTCTAAAGATGCAGTAAAACATAAAATCGGAATGGTGCACCAGCACTTTAAGTTAATAGAAAACTACACGGTTTTAGATAACATCATTCTAGGGAATGAAAGTAGGTTTGGGTTTTTACCTTTAATTAATCGTAAAGTAAGTGAAGCAAAGATTAAAACCATCATGGAAAAATATGGAATCTTTGTTGATCTTAAACAAAAAGTTAGTAACTTAACAGTAGGTCAGCAACAACGGGTTGAGATCCTAAAGGTTTTATTTCGTGATAGTAATATCCTCATCTTTGATGAACCCACTGCAGTTTTAAGTGATCTTGAAATTCAAAACTTTCTCAAGATTATTGCTAACTTTAAAAAGCTAGGAAAAACAATTGTTTTAATCTCTCATAAATTAAATGAAATTAAACAAGTTGCTGATACAGCTACTGTCTTAAGACTTGGCAAGGTAGTTGGTAGATTTGATGTTAAAACAACACCAGTTGATAAGATTGCGCTTTTAATGATGGGCAAAGAGTTAAAACAAACTAAAAACACCACAGATTTTGTTGCTAAAGATGAACCTGTTTTAAAAGTTCAAAACCTGAATTTGTTTCTCAATAAATCTTTAGCATACAAGTTCTTAGTGAGATGCAATAACATCCATAAAGCCCAACAAATTAAGAAAAATAAACCATTAAAAGACTTATGGATAATTAGTTTTTTAAATAAACTAACCACCAGTAACAAAACCCCTAAATTAGTAAAAGGCTTGATTAATAAGTTAGGACTTTCCTATCAAGAAAATACAGATGAAACCATTAGTTTTGCTATCCATAAGGGAGAAATTTTTGCTATTGCTGGGGTTGAGGGTAATGGTCAAAGTCAGCTTGTTAATTTAATTTGTGGAATTGAAAAAGCTGCTAGTAATAAGTTAATTTTTAACAATATTGATATCTCAAGATGATCAATTAGAAAACGGATTAATGCTGGGATTAGTTTTGTTTTGGAAGATAGACATAAATATGGCTTGATCTTAGATCAAACCGTGAGGTTTAATACGGTTAATAACCAGATTAATAACCGTCCTTTTAGTAGTTGAAACTTTTTAAAACCAATGGAGATTGCTCTTTATAGCAACACTATTATTAAAAAGTTTGATGTTAGGGGCAGTGCTGAGGGTAGTGCTGTTGTAAGAAGACTTTCAGGTGGTAATCAACAGAAACTAATTATTGGTCGAGAAATGACCAAACAAAATGACCTTTTGGTGTTAGCACAAGTAACCAGAGGCCTTGATATTGGTGCTATTGCTTTTATCCATGAAAACATCTTATTAGCTAAAGCTAATAATAAAGCTATCTTATTGGTTTCATATGAACTTGATGAGATCTTAGCACTTGCTGATACAGTGGCTGTTATCAATAAGGGGAGAATAGTTGGTATGGGAAAAAGAGATTTAATGGATCGCCAATCGATAGGTAGATTAATAATGCAATAAAAGACTATGACAATGTGGCAATTTAAAAGTTACTTTAAACACCACCTGGTGTTTTGAAAAGACCGATTTTTATATAGCTCTGAGAAACAAATGCAAAGAAGAAGTATCCTCTCTTCAGTGGTTTTGATAATCCTCTCTTTTCTTATATCGTTTTTACTGATTATTTCAATTCCTGGAGGTAGAGGTGCGAGCTTCTTTGCACTGTTTACTAAGTTATTTTTAGATAACACTAATACTGAAAATTTCTTAAGACAGATTGCTATTTATATCCTAGCTGGATTAGCATTTAGTTTCTGTATGAGTGTTGGTATTTTCAACATTGGTATCTCAGGGCAGATGATGGCTGGAGCCATCTTTGGGTTTTTAATGATTCTCAAGGTGTTTCCAAGTTCATTTCGACCTGGTTTTGGAGGTCAGATTATTACTGTATTATTGATGGTAATAGGTAGTGTTAGTGTGGCAGTTGTTGTTGCAACTTTAAAGATTTTCTTCAAGGTTAATGAAGTTGTAAGTGCAATTATGTTGAACTGAATTGTAGTGCTTATTAGTGCTTATTTAGTAGAGACTTACATTAAAAATAATAGTGGGGGTACAGCCCAATTCTTTTCCTTACCACTCCCTGATGAATTTGCTTTATATAACTTCTCTCCTTTAACAAAAAAGTTTGGTTGATTAGCTTCACTTATTATTGCTTTCATTAGTGTTATTATTGTGGCAGTAGTATTAAAATACACAGTTTTTGGACACAAATTAAAGTCAATTGGCAGTAGTGTATTTGGTTCTCAGGCAATGGGTTTTAATGTTAAAAAATACCAGTTCTTATCGTTTATTATCTCAGGAATTTTATCAGGACTATTAGCAACGGTTGTTTACACTGCATCAACTGAAAAAGTATTGACATTTAACAATGTTGGGGATAGTGCTATTTCAGCAGTACCAGCTACTGGTTTTGATGGGATTGCGATTGGTTTAATTGCTTTAAATAACCCCTTTAGGATTGTTATTGTTTCTGTTCTTATTGCTTTTGTTAACATTGGGGCAAGACCTGCTAATTTAAACCCTAGTACTGCTAGTTTAGTTTTAGGAATCATGATGTATTTTGCTGCACTTTATAACCTAATGGTTTACTTTAAACCATGAAGATACCTAGTGAAGCTGAACATTGGAAAGATAAATCTCACCACATATGAAACATATGAAAACAAACTAGCTGCTAACCTAGAGTGACTAAGTTTCCAACGCTTCTTGTCAAAACAGAAAAAAAAGAATGACAAAACTAAATTTAATTGGTTTGATACTAGTTTATTTGAACAATATGCAAAAAACAAACAAGAAATTGTTCAAGAATACCATCACAATTGTGCAACTAATTTAATTGCTTGGTGATTGAATGCAATCAAAAGTGGCAATATTAAACCTTCAACTACTTTTAAGTTGGAATTTGTTAATTTTAAACACCAACAGAAGTTTGTATTAAATTGGTTTAAAAATGAAAGTGAATCACTGCGTGATTTCCAATCACAGTTTGAGAGAATCAATAAGTTAGTGGAAAGGGAGTTTGTTAAGTAATAATGTTAAGTTTAGCACAATTAGAAAGTTGGTTTTTTATCGCTCCAGCACTGCTTTTAGCAGTATTGAGTGGTTATCTTGCTGAACGCGTTGGGATCATTAATATTGCTATTAATGGTGGAATGGTGTTTGGTGGGTTATTTATGGCACTCTTATCGTATGGATTTACAAATAACTTAAACCAATCCGCTCCCTCCTGATCACTATTTATCACCATTCCCTTATCAGTTTTATTTAGTAGTGTTATAGGTTGTTTATTTGCACTAGCAGCAGTTAAGTTAAGAGCAGATCATGTTATTGTGGGAACTGGGATTAACTTGTTGGCTAGCGGAATTACCCTTTTTATTAGTCAGAATGCTGCTAGTTTGTTTTCCGATACTACCTTAAGAGTAAGGTACTTATTTCCCATCCAAACTACTGTTAGTATAGAAGCAATTGGTGTGTTTGTTTTTAGTTTACTTCTGATTGGGTTTGTATGGTACTTGATGAGTTTTACTAAAACTGGGTTGAGATACCGTGCGGTAGGTGAGAATCCTAATGTCATTGATACCCAAGGGATTAGTGTTTACAAATACCAATGGATAGGCGCAATTTGTTCAATGATGGTAGCTGGATTGAGTGGTAGTTTGTTTGTTTTAAGTGTTTCTAACTTTCCCTTTAACAGCGGAGATGTAAATGGCTTGGGTTTTATTGCTATTGCCATTATGATTATCTCAATGTGAAGAATTATCCCTAGCATCTTTATTGGGTTAATCTTTGCATATGCCTATGTTTTTACCAATAGTCAAATAGGATCTAATAGTAATTCCTACTTGTTAAGAACGATCCCTTTCATCATCTCATTACTAGTAATGTTGTTATTTGGTTTTCTTAATGTTGCCCCAAAAAATATAGGTAAACATTTTGACAAGGGTTTAAGATAAACAAAAACCTTATTTATAGTTAAGTAAGTAGTTTTATTAATGATTAAAAACCTGGTGGTGATTGAATCACCCAATAAAGTTAAAACATTAAAACAATATCTTCCTAGTGATGAATTTGAGATAGTCTCAACCGTTGGTCACATCAGAGAAATGGTGTATAAAAACTTTGGTTTTGATGAAAATACCTATACCCCTATCTGAGAAGATTGAACTAAAAATAAACAGAAAAATCCCAAACAGAAACACCTGCTCAGTAAGTTTGAGATCATCAAATCAATCAAAGCTAAAGCTAGTGATGCACAAAACATTTTTTTAGCTTCTGACCCTGATAGAGAAGGGGAAGCCATCTCTTGGCATGTCTATGATTTATTGGATCAAAAAGATAAAGCTAAGTGCAAACGAATCACTTTCAATGAGATCACTAAAAAAGCAGTAGTAGATGCATTAAAACAACCGCGTAACATCGATCTTAACTGGGTTGAAAGTCAGTTTGCCCGCCAAATCCTTGACAGGATGATTGGTTTTAGATTATCAAGATTATTAAATAGTTATCTGCAAGCAAAGTCTGCAGGTAGAGTTCAATCAGTGGCTTTGCGCTTTCTTGAGGAAAGAGAAAAGGAGATAGCTAAGTTTGTTCCGCGTTTTTGGTGGACAGTTGATGTTTTATTAAACAAAGAAAATAACCAAAAAGTAGTTTGTGCAAACAAGTCTATTTCTTTGGTTTTAAGAGAAATTAACCCTGAATTAAGTGCTAGTTTAAAACTGGGTTTTGAAGCTGCTGAAAACGTATCAGGAATTGACTTTTTAAATGAAGCTTCAGCAACCAGATTTGCCAACCAACTGACTGGCGAATATGAAGTTTATTTTATTGATGAACCTAAGATTTACTATTCATCTCCAAACCCAGTTTATACCACCGCTTCACTTCAAAAGGATGCAATTAATAAGTTAGGATGGTCTTCCAAAAAAGTAACAATGGTGGCCCAAAGACTGTATGAAGGGATTAGTGTTAATGGGAAACAAACTGCATTAATTAGTTATCCAAGAACTGATTCAATTAGGATTTCAAACCAATTTCAATCAGAGTGTGAAAAGTACATTGAAAAGGAGTTTGGAAGTCATTATTTAGCTGATAAAAATAAGTTAAAAAGACATAAAAAGGATGAGAAAATCATCCAAGATGCCCATGAAGGGATCCATCCTACTTACATTACTATTACCCCCAATGATCTGAAAAACGGGGTGAAACGCGATGAGTTTCTCCTTTATCGTTTAATATGGATTAGAACAGTTGCCAGTTTAATGGCAGATGCTAAAACATCAAGAACTATTGTTCGTTTTATAAACCAAAAAAACAAGTTTTATACCTCTTCAAAATCACTTTTATTTGATGGTTATCAAAGGTTATATGAAGAGATTAAACCTAATACTAAAGATGAACTTTACATTGATCTTAGTAAGCTTAAAATTGGTGATAAATTTAGTTTTGAAAAGATCAGCGTTAATGAGCATAAAACCAACCCACCACCACGTTACACCCAAGCTAGTTTAATTGAAGAGCTTGAAAAATCTAACATCGGTCGTCCCTCTACTTATAACACTATGGCCAGTGTTAATCTAGAAAGGGGCTATGCTAACTTAGTGAACCGATTTTTTTATATCACTGAGCTTGGTGAAAAAGTTAATAATGAACTTTCCAAGCATTTTGGGAATGTAATTAATAAAGAATTTACCAAGAAGATGGAAAAATCTTTGGATGAAATTGCTGAAAACAAAGTAAACTATCAAGAATTTCTTAAGCAGTTTTGAACAAATTTTAAATCTGATGTTAAACTAGCTGAAAATTCAATTCAAAAAGTGAAAAAGGAAAAAGAATTGGTTGAAAGAGATTGTCCTAAATGTAATCAACCGTTGGTATATCGTTACACCAAAAGAGGTAATGAGAAGTTTGTTGGTTGTAGTGATTTTCCTAAGTGTAAATACAGTGAGTTTAGTAATCCTAAACCAAAACTAACCTTGGAAACACTTGATGAATTGTGTCCTGAGTGTAACAATAAACTGGTTAAGAGGAGAACTAAATTTAACGCTAAAAAGACCTTTATAGGTTGCAGTAATTTCCCTAACTGCCGTTTTATCAAAAAGGATAATGCTGCTGAATTTAAACAATAACAGCGTTCTTATTGTTGCGTTTGTAATTGTTTCTTTATTCTTTCTAATAATTGTTGGGTTTGCTTTAAATTTAGCAATTGCTTTTTCACTCCATTTAAAGCAGAATAAAAATAACAAAAAATACATCTTAAATGACCAACAGATCCAGTTAAGATTAACTGAAAAACAAGCCCAATTAACAACTTTACTTAACTTTTATCAACAAAAAATTGAAAGTGTAAACAGAGAAAAAAGTTGGTTAGAAAGTCAGTTACAGGTAATTGATAAAAAGGATTTAAAGCAAGCGCAAAAGTTAACTTTACATTTAAAAAAAGACCAAATATTAGCTCAACTTAATGAAAAGCTGATTCAAAAAAAAGTTGATCAGCCTTTAGTTAATGAACTACAGAAAACCAAACTTTCCTATCTTGAAAGGTTAGTTGATCAAAAGATTAAACTCAGTGAAAATAATTTCAAAAGTGCTTTTCTTAAAACGAAGGTAAAAGAGACAGCATTTAATATCTTTGCAGCTAAAAACAAGGTTAACTGGGAGTATTTTAAACAGGTGTGTGATGCTGATTGCACTTTAAAAAACTTAGAAGATGAAGTGGAAATTACTTTTTCTAATTGGAGTTATTTGAGAAGGATGCAAGCTCTATTAGCGTTTGAAAAACTAATTAGCAAAATCAAAACAGTCAAAATTAATGAATTGGTTATTAATGAAACTTTAGATGAAGTGAAAAACGAAATTAGCCAAACTGCTTTTCAAGCGGGTGAAAAAATAGTTAAGGAATTTCAGATAACTAATTTAAACGAGCAAATTACCAGACTAATTGGCTTGCAAAAATACTATTTTGGCACTGATCAGCTCAATCTCTTAGAACTCGCGGTATTAACAACCAAATTAGTAATTTTACTGAACAAAAAGTTCAAACTAGATTTAGATTTAGAACTTCTTAAAGCAGCTAGCTTGTTTAACTATTTAAAGTGAGTAGATAATAACCAGTTCTTTCAAACATTAAACACAAAACTTAACCAGCTTTTAATTAGTGATCAAGTTATTGCAATCATCCAACAACAAGAACTTAGTTTTTATCCTGATCAGTATGGGATGTTAATTAATGGAGTGAAAACAATGATTAGAGAGCATAACACCATTGATTTTGAAAAACTGGTTTTTCTCAACTCAACAAAACTTATTGATAATTTTTATCTCTATGATTTAAATATGATCCATGCAGTTGAATATAACAACTGCTTTTATTACTTTGTTAGTGTTAAACCCTTTGAGATTAAGTCATTAGCAGAACTTGATCTTTTTGTTGTTTTATTAAAAACTTTTTTAGCTAAAAAGCAAAAGCAAAATCCAAAAGCGGTTAAACTGTTTATCACTACCAAAATTTTAGCGATCTAATTACATTAGATTTTATTAGAATTGTTTCAACTAATAAATTGGCCTTATGGTAACAGAAATTAGAAGTCTTAAACAACTTGAAGAGATCTTTTCAGCTAAGAAAAATGTTATTGTTGACTTTTGAGCAGCATGATGTGGTCCTTGTAAACTAACCAGCCCTGAGTTTCAAAAAGCAGCAGATGAATTTAGTGATGCTCAGTTTGTTAAGGTTAATGTTGATGATCATACTGATATAGCAGCAGCTTATAACATTACCTCTTTACCAACTATTGTTGTTTTTGAAAACGGGGTTGAAAAAAAGAGAGCTATTGGCTTTATGCCAAAAACCAAAATTATTGATCTTTTCAATAACTAAAGTCCATGATTGATCTGCTTGGTTTGGATCTGGATGGAACGTTATTATCTAAAACTAAAAAAATTAACAATCCATCAAAATTAGCATTAACTAATTTAATTGCTAAAAAACCAAGTTTAAAGGTGATGATTTTAACTGGTAGATCAGTTTTTTCTACTCTAAAACACGTTGAAAAGCTGAACAGTTTGTTTAAAAAACCAATTGTTGATTATTTTTGTTGTTATGGGGGTGCTAAACTTTATCAAATTGAAGCAAATAAGCCACAAGAAAGATACAAGTTTTGCTTGGAAAACAGTGTTGTTGAAACTACCTTTAGTATTATCAAAAAACACCGCGGATTATGTTTAGCTTACTTAGATAGTTATGTCTCTCCTTACCTTTGTTTAACTGGTAACAAGCTCCTTGGGTGGTTCACTAAATACTTTTGGTATAGAAAAAGGTGTGTGTTTTTTAACCAGAACCATTTAAAACAAGGTATTCTAAAGATTAGTGTTTACTTTTTAAGTGCAAAAAGGTGTAAAAAAGTTTATGAAATCTTAAAAAATACCTTTCAAGAAAAGGTTAATGTTTTAAGTTTTTCTAATAATTTAATTGAGATAACTCATCATGATGCTAATAAGGGTTATGCAATTGAATATATGGCCAAAAGAGAACAACTTTCACTTAATAGAATAGCAGTTATTGGTGATTCTTGAAATGATTATGCAATGTTCAAAAAAGCTAAATATTCCTTTGCAATGTCAAAATCCCCTTCCCAGTTAAAATTAATTGCTACCAATACCAGTAACAAAACCAACCGTTACCGCTTTAGTACCTTACTTAATTTAATTAGTGAAACAATCATTAATCAAAAAGCTGATTAGATGTTAAACCAAATGCTTTATAAAACATTTTTAATGTTTCATCAGCAACTTTTTTAGCTTGGTTTTTACCATCATTTAGGACTTTTAATACCATTTCATCAGTAATTTGTGCTTTTTTAAATTTAAGTGATTCTATGACATTAATAACAGCACTACTGAGGTCATTCTTTAAATCTAAATAACTTTTATTCTGATAATATTTAACAAGATCAGAGCCGATTTTTTTACTTAAATTATGGTTAACTTCTTCTTTTAAAAGTGCAGTTAAAATAACAAGTAAATTAGTAACACCAGGTTGGGTTTTTTTAGCAAAACGAATCTTATTAAAACTATCAGTTGTGGCTTTGCGCACTTTTTTGATGATTGTTTCTTTACTATCATCCAGATAGATAACACCATTTTGATCAGGATTTGATTTGGACATCTTCTTTAAAGGATTTGATAGATCCATGATCCTGTTGGTATCTTTGTTTTCTATAAATACAGGTAATTTCAGTTTTAACTTAAATTTTTTTGCTATACGTTTAGCTAAATCATTGGTTAATTCCAAGTGCTGCTTCTGATCATTACCAACTGGAACAATATCAGGTTGATAAAGCAAGATATCAGCAGCCATTAACACTGGGTAAGTTAACAAACCAGTTGGGATAGTAATGGTGTTATTACTATTTCTTTTTTGCGCTAATTTCTTTGTTTTAAATTGGGTCATTCTTTGTAATTCACCTAGATTACTTTGTGTCAGCATTAGATAACCTAACATGGTATGTTCCATCAGATCACTTTGTAAAAATAAGTTCACTTTTCCATAATCAAGTCCTAGTGCTAATAAAGTTTTAACAAGTTGCAAGTTGTTATCTTTGAGCATTGTTGGTTCAAAATCAACAGTAATAGCATGAAGATCAGCAACAAATAAAAACAGTTGGTATTGACTTTGGAGTTGTTTTAAACCTTGCATTACACCAAGAAAGTTACCTAGGTGTTGTCTTCCAGAAGCTTGAATCCCTGTAATTGCGCGCTTTATCATTATTAGAATTGATAGGGTAATTAAAGGTTATTTTAATGAAAAAAGCGGGTAAAAAAAATAGTGATGCAGGTAAGACTTTTATCTTAATCTCATCATCCTGTTCATCCTGTCAGAAAGCCATTGAGTTTTTTGATCAAAACAAAATTAGCTATGTTGTTGAGAATTTTTATAAAAAACCAATTAGTGATAAGCGTTTTAAAGATATTTTAAGTCTTAGTGAGGATGGTACTGAAAGTTTGTTTTCCAAACGTGCTGATCAGATTAAAGCAACTAACAGTGTTAGTGTTGAAGAGTTGAGTATCAGTGAATTAATTAAGCTAGTTAGAGAACGTCCTTCTTTACTAAGAAGGCCTATTATCATCCAATATAATTCTTCAGGAATTCCTAAAAGGATGCGAATTGGTTATAACTCATCTGAAATTAAGGTCTTTGAGCGTAAACTAATAGAACCAAAGCCTATAATACAACAATAACATCTAAATGAAATACAAAATTTTTGCTTCCACAACGCCCCAAACTGAACCTGTTCTCAATAAGTTAAGAACAGTTTTAAAAACTTGGCAAGCGGTTGAGAATGGTTATGAATATGTGTTTGTTTTAGGTGGGGATGGTTTTTTTGTTTCCACACTTGCTAACTATAACTGTGATAGTTGTAAGGTAGTTGGTATCAATACTGGCCACCTTGGTTTTTACACTTCTTTTAATGGAGATGATCTTGATGAAAATTTCATTTCAAAACTAACTAGCTTTGAGTTTAGAAAGATTAATTTACTGGAAGTGAAAACTGAAAACCACAGTTTCTTAGTTTTAAATGAACTTGCAGTTTACACCAACACTGCATATCCAATTAACATCTTTATTGATGATAACCCCTGGGAATCATACCGCGGTTCAGGGTTGCTAATTGGCCCAAGAACAGGCTCAACTGCCCTAGCAAAATCCGCTAAGGGAGCGGTTATTTTTCCAAATGTTGATGTTGTTCAAATTATTGAACTAAACCCCTTATTACATCCCAACCAAATCACAATTCAATCTCCGATTATTCTGCCAATGCAAACCAAGGTTGAATTTAGGATCAAAAAGGCTTTTAAAGCAGAGCAATTTCCCAATTTTTATGCAGATGGAATCAAGCTTGATTTAAAAAATGAAGATACCAGTATTAGCTTTCAGTTGGTTTTATCAAGATCAATGTTTCACGCTTCTTTAAAAACTAAGGATTTTATTGATAAACTAAAGTCAACTTTTATTAAGCAAAGCTAATGAAATGGTTGTTGTGATTGGGTTATATTTTTAGTTTTGGTTTACTCTATCTTTGGATAGTAAAAAAATCAAAACAAATTGCTCAACAACCCAACACAAAACTGGTTGAATCAACTTCTATTCCTTTTAAAGTTAAGGACTTTGTTAGTGCTTGTGGTGGTAAGAAAAACTTTGTTAATATAAAAACAACACCAACTCAATTAATAGTTACTTTTAAAGATGTTAACTCAGTGAGCTTAACAAAGCTTAATGCGCTTAATATCAAAGGAATTAACAAAAACCAAAACCAATTTCGTTTTGTACTTGGTAACTTTGTAAATGAATTGAAAAAAAAGATAGAAGATGAACAATAACATTACTAACAGTATTGTCCAATTGTTTTTCAACACTTCTCTCTTTGCTTTTCTGTTTTTAATCATTATTGCGTTTAATTTATGCTTGTTTGCCTATCTTTATTTTCAGTACCGAATTTATAAGAAAAACCCTAAAAAAGCTAACAACTTTAAAGCGAATGAATATGAAAAAATTAAGCTATTAAAAAACCAAAATTTCACTGAAAGTAATAAATTAATTGCAACAACTAATGAGTTAAATGAACTTACTAGTCAACTAGATAATATCTTGGTTAGGATTATCAACAAACCACTAGCAAAGTTAGTTAATGATTTTTTAGATGAACAGATTAAACAGATAGTTAAGCTAGATAAAAACAGTTCTGATTTTCACTCAGAAAGTGATAATCTCCCTTTTTATACCAAACTCTTTAATGATTTTCACTTTGGTGTTGATAAATTAATAAACATTAACATAAAAAACCCTCTTTATAACTGGGTTTATAGCCCCAGTTTTTTAATTAGTGAAAGTGATTTTCGCAAGCTTAATGGTATTAGTGGTATCAATAAAAAGCTTTTTGTTGAAAAACTTAGAATTGAAGACATTGTGTTTACAGATCTCAACAAAAAGTATGAAGTTAATGTCTTGACAGAAAGTCCTATTAAAGCACAAAAAACAGTGTTAACTGTGCGTAATATCCTGATGAACGATTATGTTGATAATGAAAGGATTGAATCATATGTCCAACAAGCTAACTTCTTTTTTACTGAGCACTGTAAAAAGATCGGTAAAGAGATCTTAGAATCACTTAATATTTTTATCTCAAGTAGTTCACTACACCGTCATTTTGGCTTTTTAGCATTTCGCTATTCATTTGGACAAAATGTCTTATCCCATAGCCTTGAAACTGCATTTTTAACTGCCCACTTAGCAGCTTTAATAGAACTTGATAGTGAACTGAGTTTAAAGTGTGGATTGCTCCATGATATTGGTAAATCTAATGATGATAATGGTAAAGAGAGCCATACGATTACAGGCGCTAAATTCGCTGAGCAATTTCAACTACCTGATGACATTAAATACACAATTGCTAACCACCACAATAAACATATTGACAATACTTATTGTCGTTTAACACAAATTGCTGATAAACTATCTGCTGCTAGAATTGGTGCTAGAAGTGATAGTTCGCTTCTTTTTAAACAACTAAAAGATGAGTTGAAAAAGATTGTTGATAAAACTATTAATAATTTTCATACAACGATCTTACTAGGTCAAAGTGGTAGAAGGTTAATGATTTGACTTGAAACTAAAAATCAAAATCAACTGTTAAGTAATGAGCAAATTATTGAAATGGTTGAAAAGATTAAAGCTGAAATTGCTAAGAATTCAATTACAAATCACTTCCCTATTAAAGTTGTAATTAGATATAACTTTGAACATAGTTTCAACACCAAAAGCTAAGGATGCAATACAGCGCTTTAATACCACTTTTTATATTACTAATTAGCTTAGTATTATTTTGTTTCAGCTTTAGAAAAAATCAAAGTGAAAATCAGATAGTAAAGATCTTATTTTTTGCTTATTGCATCGATTTTTTAGCTTTAATTCTTGCTGTAATGTTGCTCACTTTTTTAAGTCATGGGTTGTTAAGTTTAGCGATTTTAATCCCTGTCTTAGTTTTTTCAATAATAATGTTTTTTGTTATGGTTATTAGTCATTATCCCCTTATGAAAAGACTATTTGGAAATTAAAATTCTAAGTACCATGGAGTTGAAAACCCCTAATTTTAAGCTAATTGATGAAAAGATTGCTGAATTTAATAAGAGCAATGAAAACCTGATTGTAAAACTACTTCAAAAAGAAAAGGAATTTGCCACAAACCAAGTTACTATTCAGTTTGATACTCAGTCAAAAAAGTCAGAAGAAGTGAAAAAACCTAGTAAAAAAATACTGAAAAATTATCACTTTCTAACAGTAGTTATGGCAGTTAATTTGCTAATAACTGTTGGTTTGATAGTTGGTTTAATTTTTGCTTTCAGATATTAGTTGGTAGTTTTCTTCTAAGGAACGTTTGTTGGTTTTATTGACATTATAGCCAAACCCTTTACCAGTTTCGTATTTGGGTACTATGTGTAAGTGAAAATGAAAAACCACTTGACCTGCAATTGCCCCTTCATTAGAAATATAGTTTAAACCAGATGGTTTTAGTGTCATCTTTAGTTTTAAAGCGATTTGTTTTGCTAATAAACTAACTGCTTGTAACTCCTTTTGATCAGTTGAAGAAAAATTAGCTGCATGTTTTTTAGGGATCACTAAAGTATGACCATCAGCTACAGGAAAAGCATCTAAAAAAGCAATGGCATGCTCATTTTCTCCAATTTTATAGGATGTAATTGAACCTTGAACAATATCACAAAAGATACAACTACTTGTGGTGTTTTTTTCCATAGTAATGGTTATTTATAACAAAAAGCATTCATAAGATTAAAAACATTGATGCAAAAATAGGGATTGATCAAATCCAAACATAACTGTTATATTTAGTTTGCGTAAAAAATCCATACACTGAATAGGAGTTTTCACCATTATCCCAAACATTTAAAAATGGAATAGTTTGGAGATAAAAAGCATAGATACTGGGATAAATGCCTGCTTTTAAAAGAAAGGATTGCAAATTCCTTGGCGGTTTGATAAAATTTAAAAAGTAATCCAACATAAATAAAGTAAAACAACGGATGGATAACATGGAGTAATAGCTCTGAAAAAGCATTCCAGTCATTAATAAAAGAACCATTAAAACTATATGGTAATTGCGTTGTTATAGTTGAAAACCGACCCTGTACATCAGGTTCTATATTAACAATATCTGTGAAGCGTGATAAAGCATAAAAGTTAAAGAAAATAACTGTAAAAACTAGATAACCTGTAACACTTAAAACCAGTGAACTGTTTTCAAATCATCTGCTTTTATGGTTAAGAAAGTAGTATCATACTAAAAAGATTGCAAGGCTATTACTTTGGTAAGTAAAAGTATCAAAATTAGTAAACCAAATCAGGCTCATTTGACCACTGTTAGCAACACTAATCAGTGCAGTAATCACCATAAAGATTTGGGTGAATAGTGAAAAAGGTGCTAGAAAAATTAAATAATAATCGCATTTATTGTTAAGATAAAAGCAACGGTAAAAAATTCCAATTGATTTTTTAAGCACAATTTTTATTTAATGATATATGAGTTTTAAAAAGATTGCTGAAATGATGCGTCAAGCAGAACGAGAAACTAAGAAAAAAACATTAGCATTTGAACAACAAGCCTTTGAATACAACTATAAAAATGGTGCGATTAAGATCACTATTTTAGGTGATCTTACACTTAAATCAATTAACATCGATCCTGTTTTGATTGATGCAAGCGACAAAGTTATTCTAGAGGAGATGATTATAGAAGCTACTAATGAAGCGGTTAGTGATGTGAAAACTAAGTATGATAACTTAGTTGAGAAAACTATGCCAAAAGTTCCAGGTCTTTTCTAATTAGATTTTCCTTTTTAATAAAGATCTTATCTTTTTTCATTTCCATAAATGTAGGGATATTAGCAAAGAGTGTCATTAGTAAAATCGCAACATAACTTAAACTAAAGCCCACAATAAACACAAGTTGTGGATCACTATTGCTTAAGTTAAGAAAGGTCGCCACATTTTTATATTCACCATTAGTTCAATCACTTGCTAAGATACCAGTTGTATTACCTGTGATCTGGTCCTTAAACACAGACACCATCAAAGCTACATAACTAAAGTAAATTAGAGCAAACACATGGATATAGAAAAATGAGATTAAAGAAAAACAGTTATCCCACAAGATAATTGCTAAGCTTACAAGTAAACTGAGAAAATGCATCATGAAATAAATCTGGTTATTTCCTGTACCAACAAAGATGAAATTGACAATATCTTGTTCATTAACAGGTGTAAAAATTAGTTCACCAAAAAGAGTAACCAAAGCTCCAAATAACCCAAAAACAGCTAACACCCCACTAATCTTTTTTTGCTTTAAAAAGACAAACACTGGCGCAATAACAGCAAAAAATGGACAAAGATCTAATAAAAATATCCGACTTAGTCGTAGTGAATTTAAGGTTTGTGAAGATTGGGAATTTTGTCCATAAACATCAGGGAATAAATAAATAATCTGCCTACCAAAAAAAACATAGACAAACAGGACAATGGCTAGTGCTTGAATTAGATAGAATCTAATCTTTAGTTTTCGAACTAAAGGAGTAAAAGCTAAAACAGAAATTACACCAGCAATAATAAGGATGATGACAAGGTAGTTAAGTGTCTGCATTACTTATTGCTTAAAATTAAGATAAATAATTTTCCAATTTTGTTTTCAATGAGTGATCGTTTAAATGATCAAGCCCAACATCGCTTGCAGAAACTTTTAAGGTTAAAACAAACTAATAATGACCCTTATTTAGTAACAAAAACTAGTCTAACCCATTCTTCAAAAAGCTTTCAAGTTGAATTTGAAAAATGTGCAGAAGAAGAGTTGAAGAAAAAAACAACTGTCTCACTAGCTGGAAGGATCATTGCTATTAGACAAACCTTTTTAATTATTCAAGATTTTGATGGTCAAGTCCAACTTTACATCAATAAAAAAATCCATCCTAAGTTATTTGATTACTTTAATGAATTTGTTGATATTGGTGATCAAATTGTTGTTAGTGGTAAGCCAATGTTAACTAAAACAAAGGTATTAACTTTAGCTGTTGAAGAGATGAAAATCATTGCTAAGTGTTTATTGGTTCCACCTGAAAAGTGACATGGACTTACTGATATTGAAACCCGTGCTCGCAAGCGCTTTCTTGATCTTACCTATAACTTAGCAATGCGTGATGTTTTTCTGAAACGCACTAAGATTATTAAATCAATCCGTAGCTTTCTTGATCAAAATGGTTTTATTGAGGTTGAAACCCCCACTTTACAAGCTGTTTTAGGAGGAGCTAATGCTAAACCCTTTAAAACCCATTACAATGCTTTAAAAGCGGATTTTTATCTCAGAATTGCTAATGAAATAGCATTAAAAAAACTCATTATTGGTGGATTTAATAAGGTTTATGAAATGGGTAAAATGTTCCGCAATGAAGGGGTTGATACTACCCACAATCCTGAGTTTACCAGTATTGAAATATATCAAGCTTATGCAGATTTTGAAGTCATGCTTGTGCTTGTTGAAAAGATGATTCAATCACTTTGTGAAAGCTTAAACCAATTTAGCTTTAACTGAAATAACAAAACGATTAATCTAAAAACACCATTTCATAAGATAACAATGGTTGAACTTATTAAGAAAGTTACAGGGATCGATTTTAATTCAGTAAAAGATGATCAATCTGCCATTTTATTAGCAGAAAAACACCATGTTAAACTAGCAAAACACCAACAAAATAAGCAACACATCATTAATTTGTTTTTTGAACAATTTTGTGAACAAACATTAATTGAACCTACCTTTGTAACCCATTATCCAAAAGCAGTTTCTCCTCTAGCAAAACAAGATCCTTCAAATCCTGAATTCACCCAACGATTTGAACTTTTTATTAATGGTAAAGAGATTGCTAATGCTTACAGCGAGCTAAACGATCCTTTAGAACAAAGAAAAAGGTTTGAACAACAACTTGAAGAAAAACAGCTTGGTAATGATGAGACAAGTGAACTTGATGAATCGTTTTTAGAAGCATTAAGTTTTGGGATGGTAAACACTGCTGGGCTTGGGATAGGTATTGATCGTTTGGTAATGTTGTTATGTGAATGTAATTCTATCCGTGATGTTGTTTTCTTCCCCCAGTTACGTGAACATAAATAGTTTTGATATTTTAATTGTTGGTGCTGGTATTAGTGGAATAGTACTAGCTAACATCTTAGCTAACCACAATAAAAGGGTTTTAATTGTTGAAAAAAGAGATCATATTGGTGGTAACTGTTATGATAAAGTTGATAGTAAAACTCAACTCTTGTTTCACCAGTATGGACCCCATATTTTCCATACTAACAACCAAACTGTTATTAACTTTATCTCACCCTTCTTTGAACTAAATAACTACCACCATCGGGTTGGTTTAAAATTGAAAAATAACCTAGATTTAACCTTACCCTTTGATTTTCAACAGATCTATAAACTAATGGGAAAAGATGGTAGAAAACTCGTTAGTTTTTTTAAAGAAAATTTCAGTTTAAATAGTCATCTATCATTAGCAGAATTACAACTAATTGATAATCCTTTAGCACAAAAACTCTATCAGTTTTTAATTAGTAATGTTTATAAACCATACAGTGTCAAAATGTGGGGTTTACCATTTGCAATGATTAATGAGAATGTTATTAACAGGGTCAAGATAGTTTTAAGTGAACAAAGCAGTTATTTTCCTGATGCAATTATCCAGGGATTACCTAAATCAGGTTATACAAACAGTTTTCTTAAGATGTTAGCCAATCCCTTAATTGATGTGCAGTTAAACTGCAAAGATAACCTTTTAGTTTATCAAGATGAAAAACTGTTTTTTAACAATACTTTAATAGAAAAACCAGTTGTTTACTGTGGTTTAATTGACAAGTTATTTAACTTTTGCTTTGGTCATTTGCAATACCGTTCCCTTGCCTTTAGTTGAAAAAGATTTAACCAAAAAAAATACCAAACTTATCCTGTTGTCAATATGCCTTTAGCTAAATCAATCACAAGGAGTGTGGAATACAAACAACTAACAAACCAAGGTTCTTTCAAACCGCAAACCATCGTTAGTTTTGAAACCCCTGGCAGCTATGCAATTAACGATCCTAGGTTTAATGAACCTTATTACCCAATTAACAATACACTAAATGATACTCTTTTTAAAAAGTACTGAAAAAAAGCAAGTAAGTTAAAGAACCTACACCTTTTGGGAAGATTAGCAACCTACCAATACATTGATATGGATAAAGCAATCCTACTTAGTATTAAAAAAGCCCAACAACTGTTAAGTTAATGGAACAAAAAAACATTAGAAATTTTTCTATTATTGCCCATATTGATCATGGTAAATCTACCTTATCAGACCGCTTGTTAGAACATAGTTTAGGCTTTGAAAAAAGACTATTACAAGCGCAAATGCTTGATACTATGGAGATTGAAAGAGAAAGGGGTATTACCATTAAATTAAATGCTGTTGAGTTGAAAATTAATGTTGATAACAATAACTATCTTTTTCATTTAATTGACACCCCTGGGCATGTTGATTTTACTTATGAAGTGTCTCGTTCTTTAGCAGCTTGTGAGGGAGTTTTATTGTTAGTAGATGCAACCCAAGGAATTCAAGCACAAACGATTTCCAATGCCTATCTTGCGTTGGAAAATAACCTGGAAATTATCCCAGTCATTAACAAGATAGATATGGATAATGCTGATATTGAAACAACAAAAGATTCACTCCATAACCTATTAGGAGTTGAAAAAAACAGTATCTGTTTAGTATCTGCAAAAGCTAACTTAGGGATTGATCAGTTAATTCAAACAATTATAGCTAAGATCCCTCCACCAAAAGGAGAAATTAATAAACCTTTAAAAGCATTACTCTTTGATAGTTACTATGACCCTTACAAGGGGGTTGTTTGTTTTATTAGAGTATTTGATGGTTGTTTGAAGGTTAATGATAAGGTTCGTTTTATTAAAAGTAATTCTGTTTACCAAATTGTGGAACTAGGGGTTAAAACCCCATTTTTTGAAAAAAGAGATCAATTGCAAGCAGGGGATGTTGGTTGGTTTTCAGCAGGGATAAAAAAACTCCGTGATGTTGGGGTTGGTGATACTATTGTGAGTTTTGACGATCAATTTACAAAACTCTTAGCAGGTTATAAAAAGATCTTACCCATGATCTATTGTGGTTTATATCCAGTTGATAACAGTGATTATCAAAACCTCAAGTTAGCGATGGAAAAGATCATAATCAGTGATGCAGCATTGGAATATGAATATGAAACATCCCAAGCGTTAGGGTTTGGGGTTAGGTGTGGTTTTCTAGGTCTTTTACATATGGATGTTATTAAAGAAAGATTGGAAAGAGAATACAACCTAAAACTCATCTCAGCTCCCCCTTCAGTTGTATATAAGGTATTGTTAACCAATGGTAAAGAGATTAGTATTGATAATCCCTCTTTGTTACCAGAACGCTCCAAGATTAAAGCAATCAGTGAACCATTTGTAAAAGTTTTTATTGATTCACCTGATCAATATTTGGGCAGTGTTATTGATTTGTGCCAAAACTTCAGGGGTCAATATGAAAGTTTAAATGAGATTGATATCAACAGAAAAAGGATCTGTTATCTGATGCCTTTAGGGGAAATTATCTACAGTTTTTTTGATAAGTTAAAGTCGATTAGTAAGGGTTATGCATCGTTAAACTATGAGTTTTATAACTACCAACATAGTCAACTGGAAAAAGTTGAGATCATGTTAAACAAACAAAAGATTGATGCATTATCTTTTATCAGTCATAAAGACTTTGCTTTTAAGCGGGCAAAAAAGTTTTGCACTAAGCTCAAAGAATTGATTCCAAAGCATCTGTTTGAGATCCCTATCCAAGCAACAATAGGGAGTAAAGTAATAGCAAGAGAGACAATCAAAGCAGTTAGAAAGGATGTAATAGCTAAACTTTATGGAGGGGATGTTAGTAGAAAAAAGAAGTTATTAGAGAAGCAAAAAGAGGGTAAAAAACGCTTGAAAGCAGTTGGGAGTGTTCAATTACCTCAAGAGCTATTTAGTCATTTGCTGAAAGATGAAGATTAACATTTTTATAATTTGTTATTCTTTTGTATTGGTTGATACTTTAACAAAGTATTCACAATAAAATTTCAACACTAATATAAACAATGATAAAGGATTTTAATCCTGGTGATTTTATTGGTAAAAAACCAACTAAAATCTATGCTTTTGGTGGTATCCAAGAAGTTGGTAAAAACATGTATGGGATTGAATATGATGATGAAATCATCATTATTGACTGTGGCATTAAATTTGCTAGTGATGATCTACTTGGTATCAATGGGATTATCCCTAGTTTTGAACACTTAATTGAAAACCAAAGTAAGGTTAAAGCATTGTTTATTACCCATGGTCATGAAGACCATATTGGGGGTGTACCATACCTTTTAAAGCAGGTTGATATTCCTGTTATCTACGCACCAAGGATCGCAGCATCATTAATCTTGAAAAAGGTTAATGAGCACAAGGATGCTAAGCTCAATAAGATAGTTACTTTTGATGATTTTAGTGAGTTTCAAACCAAACACTTCAAAATTGATTTTTACCGGGTAAACCACTCGATTCCCGATGCTTTTGGAATCTGTGTGCAAACCCCTAATGGCAACATTGTTCAAAGTGGTGACTACCGGTTTGATTTTGCTGCTGGGAGTGAGATGTTAGATGTTCATAAAGTAGTGAAAATTGCTGAGCGTAATGTCCATGTTTTTATGAGTGAATCTACTAATGCTGAAGTACCAGGTTTTTCCCAAAGTGAAAAGTTAATTTACAGAAACATCCAAAAGATCTTAAAAGAAGCAAGAGGTAGGGTTATTTTAACTACCTTTGCATCCAACATCACACGGATTAATGAAATTATTGAGATTGCTTTAAACAACAAGCGTAAGATCTGTTTATTGGGCAAATCAATGGATGTTAATGTCAATATTTCGCGCAAAATTGGATTGATGGCAATTGATAGTAATGATATTGTGGAAGTCCGTGATATCAAAAACTATCCTGATCATAATATCTTAATCTTGTGTACTGGTTCACAAGGCGAGGAGGCTGCTGCTTTAAACACGATGGCACGTGGTAAGCATAATTGGGTAAGCTTAAAATCAACTGACACCATCATTATGTCTTCAAATCCAATTCCAGGTAATTATGCTGCAGTTGAAAACTTGCTTAATGAACTATCTAAGTTTGGTGTTGCTATTTATGAAAATTCATCCCAACTAAAACTACATGCCTCAGGTCATGCCACCCAACAAGAGTTACAGTTGATGCTAAATTTAATGTTTCCTAAATACTTAATTCCTATCCATGGTGAATTTAAGATGATGCGAACCATAAAAAACATTGCTAATGAATGTGGTATTAAAAGCGAGGATGTGGCACTTTTAAGTAATGGCCAAGTAATGTATTTAATTGATGAAGAACTTTATTATTCCAATGAAATTATTAATGCTGATCCTATTTATATAGAGAGTCATAACTCTTCTCCTGATCTTGCAAGAATAATTAAGCAAAGACAAATCCTTAGTCGTGATGGGATGTTTGCTGTTATTGTTGTTTTTGATAAAAATAACAACATCATTGGGATTCCAACCTTAATAACAAGAGGTTGTTTCTTTGCACTTGATTCCAATCCTTTAATGACAAAGATAGCCCATTCTGTTAAAAGAACTTTAGAAAGTGTTATCCAAAGTAAGAAGTTTAATAGTCATGAACAACTAACAAAGGAATTGAAACGAGTTTGTAAGGAAACTGTTTCTTACTTTATCTGAAAAAATAAAAACCGTAATCCCTTAATTTCAACTGTGCTTTCCTGGATCTAATCCCCCAACCTCTATTTTGTGTTACTAGTGCCTAAGGTGGTAGTGGAGTATCACAAGCTCAGTAAGGATGTAGTCAAAGAGAGTTTGGAAGTGGAAGCAACTTCCACCTTCAACCCCACCCAAAGGTTGCAAAAAGATAGTCCAATGAAGGATTCAGCAAGGGAAAGTCAAGAGAAACTCCAAGAGACCATGTCATCAATGAGTGGTATGGCTACATCCACTCGAGCCAAGGCCCTTAAGATAGAGGTGGAAAGGGGGAGTAATGTCAATCAAGGCGAACTACAATCCAACGACTTTGCTAAAAAGCCGTTTAAAGATGAGAGCAATAAGAAGTTGGATGCGAGTGGGGAGTTTGCAGGAGGCAAGGCCTGAAAACCGGTGTTGACTACCGAACAAATAAAAAAAAATAGGGGGATGGGGGCGACTTAGACTTTCTCCCCTGAATCGGCAACAACAAACCCTTCTCCAACTCCCCCTCCCCTTCAACTTCTGCTTCTTCTACCCCCCTCCCCACTTTTTCTAACATCAATGTTGGGGTTAAATCAATGATCACTCAACACTTAAACAAAGAAAACACCCGGTGGGTGTTTACCCCTAACTCTTCACCAGACATTTGAACGGGAGCAGGGTATCGCAAGGGTTCATCAAACACCAACGGCATCCCTTTTGAACAGGTGAAGCCTAGCAATAGTAGTACCACCCCAGCTGGTGGCTCCTACCAAAAAAACAACCACGTATGATGCTTTACCCAACTCCATCAGTCCCACCAGTGACTGGATCAATGCATTGACTTTCACCAATAAGAATAATCCACAACGAAATCAGTTGTTGTTAAGAGCACTGTTAGGAACGATCCCGGTATTGATCAATAAGAGCGGAGGAAGTGGGAATGAGTTTAACCATACGAATGATCAGAAGTGGGATAAAACGAATGAAAAAGATGGGAATTTACCTGGGTTTGGGGAGGTGAATGGTGGTTTTTACCCAGTTTTACTTATTTAATAGTTAAAAAAGTTTTAAATTTTTCTTAGTTTTTTATCTATTTAATATTTAAGAAAGTCTCAAATTTTTATCAGTTTATTGGTCAAAAAAGTCGCAAATTTTTCTTAGTTTTTTATTTGTTTAATAATTAAAAAAGCGTTAGGTTTTTATTTATCTAATTTCCAATTAATAAACTTCGCCACCCCTATCACCGATTCAAAGGCTGATCTGGTTAGTTTGGCACAACTTGATTCTTCCTATCTCATCTCCGACCAAACCATCCATAACACCAACTTGTTTGTGTTGTTCAAGTCTAGGGATGTGAAGCTTACATACAGTTCAAGTGGCTCAAATAACCAGATTAGTTTTGATTCAACTAACAACAAACCCTCCTACATCGTCGAGTTTACTAACTCCACTACGGTAGGCATCAAGTGAAGCGTGGTGAAAAAGTATCAGTTAGATGTGCCAAGTGTTTCAACAACCATGAACCAAGTTTTGCAAGAATTGATCCTTGAACAACCTTTGACTAAATATACCTTAAATTCGAGTTTGGCCAAACAGAAGGGTAAGACACAAGCAGTGGTGCATCTTGGTTCAAATATGGCAAATCAGTGAACCACTCAACGTAACCAACATGACTTAGACAACAATCCCTCTCCCAATGCATCAACTGGATTTAAACTCACTACCGGCAACGCATATAGAAAATTAGATCAATCCTGACCAATTTACCAACCAATTGATGGGACCAAGCAGGGCAAAGGGAAGGATAGTAGTGGGTGGAATAGTGAAGAAAACAATGGCAGCAGGGGATGCGCCCAGTGTTACTGCAGGGGGAAGTGCTTCTGGATCATTTAATAAATACCTCAACACCAAACAAGCGTTAGAAAGCATCGGCATCTTGTTTGATGGGGATGGAATGAGGAATGTTATCACCCAACTCTATTATGCTTCTACCAGTAAGCTAGCAGTCACTAACAACCACATTGTCGTGATGGGTAACAGCTTTCTACCCAGCTTGTGGTACTGGGTGGTGGATCGGGGTGCAACAACTGATTCATCATCAAAAACCCACCTGGTTTGCTAACACCAATTCTAAACTGAGGAGAAGACAAACAAAAACAATTTGTAGAGAACCAGTTGGGGTATAAGGAAACTACCAGTACCAATTCCCACAACTAGTAGTAGTAGTAGTAGTAGTACTCAAACCAAAGACCAAGCACTTGCTTGATCAACAACAACTAGCTTAGATAGTAAAACGGGGTATAAGGATTTGGTGACCAACGACACGGGTTTAAATGGTCCAATCAATGGGAGTTTTTCAATCCAAGACACCTTTTCCTTCGTTGTTCCTTATTCAGATAGTCATAGTAATCAAACTTCATCAGGAACCATTAAAACGGCTTATCCGGTGAAAAGTGATCAAAAATCATCAGTAGCGATCAATTCTTTGATTAACGCTACGCCGTTGAATAGTTATGGGGATTTAAACATTAAATAAAGCGAATTTACCCAAATTATTTACTTATTTATTAACTATTGTTACCCAATTTTTCTCTTTTTATTTGTTGTTTTTTTACTAATTAAATAAGCAGTCTTTCTTACAAAAAAGAAAAATTCATATATAATCTTTGCGCTGTTAACACCTTTGTTAACGCCAAAAATGTTCTTTCAAAACTGGATGCAATCTGTCAATTTTTTCTGAGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCATGCCTAATACATGCAAGTCGATCGGAAGTAGCAATACTTTAGAGGCGAACGGGTGAGTAACACGTATCCAATCTACCTTATAATGGGGGATAACTAGTTGAAAAACTAGCTAATACCGCATAAGAACTTTAGTTCGCATGAATTAAAGTTGAAAGGACCTGCAAGGGTTCGTTATTTGATGAGGGTGCGCCATATCAGCTAGTTGGTAGGGTAATGGCCTACCAAGGCAATGACGTGTAGCTATGCTGAGAAGTAGAATAGCCACAATGGGACTGAGACACGGCCCATACTCCTACGGGAGGCAGCAGTAGGGAATTTTTCACAATGAGCGAAAGCTTGATGGAGCAATGCCGCGTGAACGATGAAGGTCTTTTTGATTGTAAAGTTCTTTTATTTGGGAAGAATGACTCTAGCAGGCAATGGCTGGAGTTTGACTGTACCACTTTGAATAAGTGACGACTAACTATGTGCCAGCAGTCGCGGTAATACATAGGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCAAGCGCAGGCGGATTGAAAAGTCTGGTGTTAAAGGCAGCTGCTTAACAGTTGTATGCATTGGAAACTATCAGTCTAGAGTGTGGTAGGGAGTTTTGGAATTTCATGTGGAGCGGTGAAATGCGTAGATATATGAAGGAACACCAGTGGCGAAGGCGAAAACTTAGGCCATTACTGACGCTTAGGCTTGAAAGTGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACACCGTAAACGATAGATACTAGCTGTCGGAGCGATCCCTTCGGTAGTGAAGTTAACACATTAAGTATCTCGCCTGGGTAGTACATTCGCAAGAATGAAACTCAAACGGAATTGACGGGGACCCGCACAAGTGGTGGAGCATGTTGCTTAATTCGACGGTACACGAAAAACCTTACCTAGACTTGACATCCTTGGCAAAGTTATGGAAACATAATGGAGGTTAACCGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCGTTAGTTACATTGTTTAACGAGACTGCTAATGTAAATTGGAGGAAGGAAGGGATGACGTCAAATCATCATGCCCCTTATGTCTAGGGCTGCAAACGTGCTACAATGGCCAATACAAACAGTAGCCAACTTGTAAAAGTGAGCAAATCTGAAAAGTTGGTCTCAGTTCGGATTGAGGGCTGCAATTCGTCCTCATGAAGCTGGAATCACTAGTAATCGCGAATCAGCTATGTCGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACTATGAAAGCTGGTAATATTTAAAAACGTGTTGCTAACCTTTATTGGAAGTGCATGTCAAGGATAGCACCGGTGATTGGAGTTAAGTCGTAACAAGGTACCCCTACGAGAACGTGGGGGTGGATCACCTCCTTTCAAATGGAGTTTTTATTTTTTTATTTATCTTAAACACCCATTAATTTTTTCGGTGTTAAAACCCAAATCAATGTTTGGTCTCACAACTAACACATTTGGTCAGTTTGTATCCAGTTCTGAAAGAATGTTTTTGAACAGTTCTTTCAAAACTGAAAACGACAATCTTTCTAGTTCCAAAAATAAATACCAAAGGATCAATACAATAAGTTACTAAGGGCTTATGGTGGATGCCTTGGCACTAAAAGGCGATGAAGGACGTGTTAACCTGCGATAAGCTTCGGGGAGGTGGTAAAAACCTGAGATCCGGAGGTGTCCGAATGGAGCAATCTGGTAGCTTGCAAAAGTTACCATTAATTAATGAATTCATAGTTAATTAAAGCGATACGTGGTGAAGTGAAACATCTCAGTAACCACAGGAAAAGAAAACGAATGTGATTCCGTGTGTAGTGGCGAGCGAAAGCGGAACAGGCCAAACCTATCTGAGGATAGGGGTTGTAGGGCTTGCATTATGGAAGTTAAAAGGATAGAAGAAGCTGTTGGAAAGCAGCGCCAAAGAGGGTGATAGCCCCGTATTTGAAATCTTTTTAATACCTAGCAAGAAACCTGAGTAGCTCGAAAAACGTTATTTTGAGTGAATCTGCCCAGACCATTGGGTAAGCCTAAATACTAATTAGTGACCGATAGCGAAACAGTACCGTGAGGGAAAGGTGAAAAGAACCCAGAGATGGGAGTGAAATAGATTCTGAAACCATATGCCTACAACGTGTCAGAGCACATTAATGTGTGATGGCGTGCGTTTTGAAGTATGAGCCGGCGAGTTATGATAGCAAGCGTTAGTTAACCAGGAGATGGGGAGCTGTAGCGAAAGCGAGTTTTAAGAGAGCGTTTGTTTGTTATCATAGACCCGAAACGGGTTGAGCTAGTCATGAGCAGGTTGAAGGTTGAGTAACATTAACTGGAGGACCGAACCGACTCTCGTTGAAACGATAGCGGATGACTTGTGATTAGGGGTGAAATTCCAATCGAAATCCGTGATAGCTGGTTCTCGTCGAAATAGCTTTAAGGCTAGCGTAAGATCACAAATAAGTGGAGGTAAAGCTACTGAATGTATGATGGCGCCACCTAGGCGTACTGAATACAATTAAACTCTGAATGCCATTTATTTTATTCTTGCAGTCAGACAGTGGGGGATAAGCTTCATTGTCAAGAGGGGAAGAGCCCAGATCATTAAATAAGGTCCCCAAAATATACTAAGTGGAAAAGGATGTGAAAGTGCTAAAACAGCAAGGATGTTGGCTTAGAAGCAGCCATCGTTTAAAGAGTGCGTAACAGCTCACTTGTCGAGTGTTTTTGCGCCGAAGATGTAACGGGGCTAAGTATATTACCGAATTTATGGATAAGATATTTTTATCTTGTGGTAGACGAGCGTTGTATTGGAGTTGAAGTCAAAGCGTGAGCATTGGTGGATCCAATACAAGTGAGAATGCCGGCGTGAGTAACGCTTGGGAGTGAGAATCTCCCAAACCGATTGACTAAGGTTTCCTGGACCAGGGTCGTCCTTCCAGGGTTAGTCTGGACCTAAGCTGAGGCTGAAGAGCGTAGGCGATGGACAACAGGTTAATATTCCTGTACTTACAGTTAGACTGATGGAGTGACAAAGAAGGTTATCCACCCCCATTATTGGATTTGGGGATAAACTATAAGGTGGTACAATAGGCAAATCCGTTGTGCATAACACTGAGTGGTGATGTCGAGTGAACGAGTGATCAAGTAGCGAAGGTGGCAATTAATCATGCTTTCAAGAAAAGCTGCTAGGGCTAATTTAACTGTAACCAGTACCGAGAACGAACACACGTAGTCAAGGAGAGGATCCTAAGGTTAGCGAGTGAACTATAGCCAAGGAACTCTGCAAATTAACCCCGTAAGTTAGCGAGAAGGGGTGCTTATCTAAAAGTAAGCCGCAGTGAAGAACGAGGGGGGACTGTTTAACTAAAACACAACTCTATGCCAAACCGTAAGGTGATGTATATGGGGTGACACCTGCCCAGTGCTGGAAGGTTAAAGAAGGAGGTTAGCAATTTATTGCAAAGCTTTTAACTGAAGCCCCAGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTAGTCGGGTAAATTCCGTCCCGCTTGAATGGTGTAACCATCTCTTGACTGTCTCGGCTATAGACTCGGTGAAATCCAGGTACGGGTGAAGACACCCGTTAGGCGCAACGGGACGGAAAGACCCCGTGAAGCTTTACTGTAGCTTAATATTGATCAAAACACCACCATGTAGAGAATAGGTAGGAGCAATTGATGCAAGTTCGCAAGGATTTGTTGATGTGAAATGTGGAATACTACCCTTGGTTATGTTTTGTTCTAATTGGTAACTGTGATCCAGTTTCAAGACAGTGTTAGGTGGGCAGTTTGACTGGGGCGGTCGCCTCCTAAAAGGTAACGGAGGCGCACAAAGGTACCTTCAGTACGGTTGGAAATCGTATTTAGAGTGTAATGGTATAAGGGTGCTTGACTGTGAGACTTACAGGTCGAACAGGTGAGAAATCAGGTCATAGTGATCCGGTGGTTCAGTATGGAATGGCCATCGCTCAACGGATAAAAGCTACTCCGGGGATAACAGGCTGATACTGCCCAAGAGTTCATATCGACGGCAGTGTTTGGCACCTCGATGTCGACTCATCTCATCCTCGAGCTGAAGCAGGTTCGAAGGGTTCGGCTGTTCGCCGATTAAAGAGATACGTGAGTTGGGTTCAAACCGTCGTGAGACAGGTTGGTCCCTATCTATTGTGCCCACAGGAAGATTGAAGAGCTTTGCTTCTAGTACGAGAGGACCGGAGCGAGGACACCGCTTATGCTCCAGTTGTAGCGCCAGCTGCACCGCTGGGTAGTAACGTGTCTATTAGATAAACGCTGAAAGCATCTAAGTGTGAAACTATCTCAAAGATTAATCTTCCCATTTCTGTTAAAGGAAAGTAAGAGCCGTTATAGACCATGACGTTGATAGGTTACAGGTGTAAGCATAGTGATATGTTGAGCTGAGTAATACTAATTGCTCGAGGACTTATTGGTTGATAAAAGGTATTTATCAACTAAAAGATTGTCGTTTTTGTTTGGTGCTAATATCGCTGTGGAAACACCTGGAACCATCCCGAACCCAGCAGTTAAGCACAGTGGAGCTAAATGTAGGTAGTAATACTGAGAATAGGTAAGCACCAAGCAAAAATTAAGGACTATAGGTTTAAAAACCTATGGTCTTTTTTTATTTAATGAATAGTTTTTATAACTAATTTATCTAAAAAAATCGAACAACCTTTGACTAAGTATACGCTCAATGGCAGTTTGGCCAAACAAAAGGGCAAAAGTCAAGTGGCGGTGCATCTGGGTAGTGGGCAAGCAAATCAGTGAAACACCATGCGCAACTCCATCGGTCTGAATGACAATCCCAGTCCCAATGCATCAACCGGATTTAAACTCGACAAAGGCAATGCATATAGAAAACTAAGTGAGTCCTGACCAATTTACCAACCAATTGATGGGACCAAGGATGGCAAAGGGAAGGATAGTAGTGGGTGGAATAGTGAAGAAAATACGGCAGCAGGGGATGCGCCCAGTGTTACTGCAGGGAGGTAGTGCTTCTGGATCATTTAATAAATACCTCAACACCAAACAAGCGTTGGCAAGCATCGGCATCTTGTTTGAATGGAACAATGGCGAGAAATGTTGTTAGCCCCCTCCTTCCATCTCTACCAACCCAACAAGGTGAAACAATACAATGCATCTAACACCTACAACAAGTTAATTGAGCCTGACAAGTGAGAATCATCAAGTGATTTGAACAATATGACCAACTTGTTAAAGCTGTTGTCTAGTAAAAACATCAAACAAAAGTTGGGGAAGGACACCCAATCAATGGGAAATAATGGAGGGGCAAGACGCCTTTTCGTTTGTTGTTCCTTATTCAATGAATCACACAAATAATGGTTCATCAGGACCCATTAAAACTGCTTATCCAGTGAAAAACACAGAGAAAGCAACTGTCAAGATCAATTCTTTGATCAACGCTACGCCGTTGAATAGTTAAAAACCTGATTTATTTTTATAGAAACAGTTACTTTGAAGATTCACTACTAATAATTTAAATTAAGCACTCATACTAATATATTAATCCCTTTAGATTGCTTAACTCTTTAAAACTTCCCTTTATAATCAAATATCGCCATGAACGACTGACAGTGACTGAAAAACAGACTTGTTAATTCCAAAACCAAATCTGTTAGTTTCTGATTACCACAAACCTCAAGTAATATCATTGATATTGCTGAATTAATTAAGTGCTGTAGTGAGTTAAAAAACACTTCTATTAATGGTTTAATTGACTTTTTGAACCAACAGGATAAACTTGAATTTAACTTAACAAGATTAAAGGAGATAGATGTTGAAGATGGTAAGCAACTATTTGGGATAGAAACTAGTGTTTATAAACACTTTCAAAATGAAATTGCTCGTTTTTATAAACAAGTAAACAAACACTTTCGTGAAACAGGTAGTGAAAGTTTGTTTTTAGCTTTACCAGTTATTGAAGGGATTAATGAGTTTAACGATATCTTTCGAGCTCCATTACTTTATGTTGGAGTTAAACTCAAAGTTTCCCCACGCTTTGAACGTTTCTGATTAGAAATTAACAAAGAAGAGATCTTTTTAAACCCTACTATTATTGGAGTTGAAACCAACAAACGTAATAGTTTGTTTAAAAATAACTATGATACTACTAAGATAGATGTTAATGATGCTTTAAAGGTATTTAGTGAACTTGAATATGAGTTTAGAATGCCTTTAACTTCTGAATTGAAGAGTTTTAGTAAAAAAGCAAAGAGTGATTTTAATACTGAAAAACGAACTAACTATCTTATTAACAACGTTCTTTTGGGGATCTTTGATGTTAAGGGTGATCAGCTGTTCCAAAACTTTAATGAGATTCTAAACACTGATCCTGATGTATTAGATGAACTTCTAAAAGATAGAAGAGATCTGTTGTTAGAAAACCGGGAATTCCGTGAACAATTTGATTTAAAAGATACCTATCTCTTCAGTCACCTTGATATCTACCAACAGTATGCAGTTAAGCAAGCTTTACTTGGTGATTTAATTATTGAAGGCCCACCTGGCACAGGGAAATCTGAAACAATTGTTAATATCTTAGTTAACCTTGTTTTAAACAACAAAAAAGTATTGTTTGTTTCTGAAAAAGTAACTGCACTTGATGTTGTTTACAACCGTCTTGGTAGTTTTAAACACATCACACTTTTCAACGCTAGTGTTGCTGCTGAAAAGAAACGCTTTTATAACCAGTTTGCTGAGTTTGAAACTTATTTCACTACTTACTTTTCCAAGAAAGATTTGGATGCTACTTTACCAACATTTGAAGGTAAATGGGTGGATGATATTTTAGGGGCATTTCAAGCATTACAAGCTCTTTATGACACCAAGATAAATTCTGGTGAAAATCTGTTTAGTTTCAAAGAGATTGTCAGTAGCTTTCAGATGTTGGATGCTAGTTACATCAAGATTAAAGAATATGAACGTTTTGATGAGTGAGTGCGCGTCTTTTCAAATCCATTGTGATTAGAAAAACACTTAAGTTACCAAGAGTTGAAAAAAGAACTTAGTCAGCGCTGGAATGGTATTGATAATTTCTATCAGTTGCAATCGCTTTTAAACCAAACCAAAAAACGAAAGGTTTTAAACTATGTGTTGGAACACTTTGAACAGTTTAATACAGTTATCAGTCCTAAGCATGTTTTGTTCTACAAGCCTAGCAATAAATCACAATTGCTCTTAAAACAACTGAAACAGGATGTTGAACAATACACTAGTTTACAACGTTTCCAATCTCCTACTAAGTTTGAAACAATCAAGTTGAATTTCATCAACCAAGTTAATGAAAACCCAACCCCATGGTTCTTTTCTTGATTTATCCAATTTCATGCCAAGCCACTGTTGGAAAAACTCGTTAGTTTTGAGTCAAACATTATTAAAACAAAACAAGCTTATCTTAATGGGATTGAAAGCTATGTAGCAAGTTGTAAGAAACTGCTTAAAACAACTATTTTAAACAATTTTTTTCAGCTTTATCAAACTAATAAAGATGAACTATTGGAGATCTGCAGACAAGCAAAAAACCCAGTTTTAAAAGAGATTACTTGGTGGTTTAAAAAACATTTTGAACTCTTAAAAAAACTCTTTCCAGTGCACATTATGACCCTTGAGTCTGCAGCAACTCTAACCCCTAACCAACGTGGTTTGTATGACTATGTGGTTATTGATGAAGCTAGTCAAGTATTTCTAGAAAGAGCAATTCCTATCTTATTTAGAGCTGATAAGTACATTATTGCAGGGGACACTAAACAGTTAAAACCAGCTAACTTCTTCCAATCACGTGCTGAGTATGATGTTGATGAGGAGTTTGAAGATGGCAATATAGAAGCTGCTGTTCACTCCAGTTCTCTACTTCATTTTTTAAAGAACCGTTCAAGAATCTTAACTTTACTTAAGTTCCACTACCGCAGTGATTCAGCTGATCTGATTGCTTTTACTAATAACAGGATCTATGACAATGAATTAATCTTTATGAATAAAGCTAATGCTGATCAAAGGGTTTTTATTGTCCATGATGTAATAGATGGTATCTGAAAAAACAACCGGAATCTCCAAGAAGCACGTGATGTTGTGCAACGCTTAGAACAACTAACAACAACCAATGATTACAAAAAGTCACTTGGTGTAATTTGTTTCAATAAAAACCAAGCAGATCTAATTGAATACTTAATTGATAAACAAAACAATCCGCTACTTAACGAGTGAAGAGAAAGACAAAATGATGTTGGTGAATATGAAGGATTATTTGTGAAAAACATTGAAAATGTGCAGGGTGATGAAAGGGATATCATCATCTTCTCTTTAGGTTATGATCGTTCAGTAAATAGTTATGGTCCTATTAGTAAGCAAGGTGGGGAAAACAGACTAAATGTAGCGATAACTAGAGCTAAACAAAGAATAGAACTGTTTAAGACAAATAGAGGGGAAGACTACAATGGTTTAAGCTCCAGTTCTTTGGGTAGTAAACTGTTAGTTGAATATCTACTTTATTGTGAAGCAATGGCTAAAAATCAGGGTGAGAAAATCACTTTTCAAGCGGTAAAAAGAAAAGAAACAAAAGCAAAGTATGAACTGGCAGTGGAAAATGATTTCTTCAACCAACTGCAGGCAATTTTTGGTGGAGAGTTTGAGATTAAACGTAACGTTAATGAAGGGGCTTACTTTTTCTCATTTGTCTTTTACTTTAATAATATCCCTTATCTTGCCATTGACTTTAATATCCCCATTCCCACTTCAAGAAAACAAGTTATGGAAGGGATTTTATACCGCGAACAGTTTCTCAAAAAACGCCAATGGAACCTAATTAACATCTGGATTGATGAGTGAAAATTAAACCCAATTGGGGTGATTTCTAAAATCAGATCAAGTTTAGCAGTGCATCAAAACCAGCATGAAGAAATATAATTAGATGAAGATAACTTTCATTTCTGGACAAGAAGTGTCGTTAGGCACTTCTTTTTTATTGTTTTCAAAAAATAGTTATGAATGAATTAAACCAACCCTTACTTGCTATTATTAAAAATGTCGCTAAAACCAAAAACCTTTCTATAGAAGAGGTGGTTTTTTGTTTGAAAACAGCTTTAGAACAAGCCTATAAAAAACACCTTAACTTTGTTAATGTTGAAGTTAACATTGACTTTGATAAGGGGATTATTAATGTTGAACAACTCTTTAATGTTGTCAGTGATGAAAATGAAGATTATGATGACTTTCTTGAAATCCCTTTACAAGCAGCTAACAAAATAAGCAGTTCATTGCAATTAGGTGATGTGTTGCGTAAACCAATCCCCTTAAAGAACATCAGTAGTGATCTTATAAATAAGATGATTGCTATCTTTAACCAAAAGATTAGTGAAACAAACTTTAAAGCAGTAATGAGCGAGTTTAGTAGTGAGGTTGGGGAAGTGATTGAAGCGAAAGTTGAAGATATTGATACTAACAAAGAAGGTGGTTTAAAGGGTTATATTATTAACCTTGAAACTACAAAGGGTTATATCTCCAAGCGGGAATTGTCAAAAGGGGAGCGCTTAGAGATAGGTAAAAAATACCTCTTTGTTATCAAAGAAATCCAACGGCAAGCATCGTTATGACCAATTACTTTATCAAGAAGTGATACCCGCTTACTACAGTTTTTGTTAACTTCAAATACTCCAGAAATTGAAAATGGCACGATTGTAATCAAAAAGATTGAACGTTCCCCAGGAGTGAAATCAAAGATAGCAGTTATCTCCAATGATCCTGCAGTTGACCCAGTTGCTGCTATCTTAGGACCTAAGGGTGAGAAGATTAGGGGGATTAGTGAGGAATTTAATGGTGAGATTATTGACATTGTTTTTTGAAATGAAGACAAGCTAAAGTTCTTAATTAATGCCATTTTACCTGCAGAAGTCATTGGTTATAACATCTTGCAGGATGATGAACGTGATACTAGTATTGAAGTTGTTGTACCTGCAAACCAAATTGCTAATGTTTTTGGTTTTAAGGGTGTAAACATTAGGTTAATTAGTAATTTAACAGGTTGAAATAGCGTTGATGTTTACAGTGAAAAAGATGCAAGTGAAGCCAACATTAAATTCACGAGGTTAAGCTTTGAACCTGAAGGGTTGTTTGGCATTAAAAAAAGAAGGGAAAAGATCATTAGTAATGATGCTACTGATAAAGTCTTTTACACTTCTAAAGACAATGTGATAGATGATGAGATTATTGTTGATTTAGCTAAAGATCTAATGGTTGATAATAAACAAAAACAACCTGAGCAAGTTGCAAAACAAGTTGTTGAAAAACCACAATTAGAAAAACAAGTTACTCCAAAAGAAAAAGAGAAAGTTCAACCAAAAGCTAAGGTTCATTCTAATAGCCATTCCAAAAAACCAGTTAAAACTAATCAGGTTTTTTCTATCACTGTTGATGCTAGTGATAAGAATCTTAAAAAAGATCAAGTTGATAATAACCAAACAAACCCCCAAACAAAACAAACATTTGATAGCTTTGATGATCTTTAATGCAACTAATAACAAGACTTTGTTTATTAACAAGAAAACATTTTGTTAAAAGAGAACTTTTACGTCTTGTAAAATTAGACAACCAACTTGAAATTGATCTTAATCAAAATCTCAAGGGCAGGGGTTATTATTTGAGTGTTTTTGGTTTAAAGATAGATAAAAAACACCTCAAAGCTGTAGTTGAAAAACACCTTAAGGTTAGTTGTAATGATGCAAAGCTTACTGCAATGATTACCGCCTTACAACAATTAGCACAAGATGAAAAAAAATAGAGCTTTCAACCAGGTTAAAAAAATAAAGTTTGATGGTAGGATTAAAACCAGTGCCAAACACCAGTTACGTAATGTTAAAACCGGGGTTAAAGATGGTGTTTTTATCTATAAAGGTCCTTTAACTGTTAGTGAGTTTGCAAGTAAAACTAATATCGCTGTTGCTAATATTATCAAGCACTTTTTTTTAAATGGTTTGGCACTAACAGTTAATTCAGTTTTAACAAATGAACAGTTAGCAGATGCATGTGTTAACTTTGGGTTTGACTTTAAGATGGAAACTGAAGTTACCCATGAAAATATTGTAGCTAACATCCAGTTTGAAGATAGTGATGATTTATTGCAACCAAGACCACCTATTGTTACTATCATGGGTCATGTTGATCATGGTAAAACTTCGCTTTTAGACACAATTAGAAAAACTAATGTAACTGCTAAGGAGTTTGGCGGAATTACCCAAAAAATTGGTGCTTATCAGGTGAAAAATCACCAAAATAAAACGATTACTTTTATTGATACTCCTGGGCATGAAGCATTTACTTTAATGCGTGCAAGGGGTGCGAAAGTAACTGATATTGTGGTGTTGGTTGTGGCAGCGGATGATGGGATTAAAAAGCAAACAGAGGAAGCAATTAGCCATGCTAAGAGTGCTAACACTCCTATCATTGTTTTTATTAACAAGATGGATAAACCAACTGCTAACCCTGATCTGGTGATCCAACAACTCAATAAGTTTGATTTAGTTCCTGAGGCTTGGGGTGGGAAAACTATCTTTGTAATGGGTAGTGCTTTAACTGGTCAAGGGATTAATGAGTTGCTTGATAATATCTTGTTGCTAGGGGAAGTGGAGGGTTATCAAGCTAACTATAATGCCCATTCATCTGGTTATGCAATTGAAGTACAAACTTCAAAGGGACTTGGCCCTATTGCCAATGTCATTGTAAAAAGGGGTACTTTAAAGTTAGGTGACATTGTGGTGTTAGGGCCTGCATATGGAAGAGTTAGAACGATGCATGATGAAAATGGTAATAGCTTAAAACAAGCAACCCCTTCAAAACCTGTGCAGATCTCAGGGTTTGACATTATGCCTGTTGCTGGGGAAAAGTTCATTGTTTTTGATGATGAGAAGGATGCAAAGTTAATTGCTAACAAGTTTAAAGAACAACAAAAACAAAAAGCTAACAACTTAACAGTTAATCAAACCTTAAAAGAACAGATTAAAAACAAGGAAATTAAGATATTAAATTTGATCTTTAAAGCAGATAGTGATGGTTCATTGCAAGCTATTAAACAAGCAGTTGAAAACATTAATGTTGCTAAGATCTCACTTAGTATCATCCATGCTGCAGTGGGGCAGATATCAGAGAGTGATATTATGCTAGCAAAAGCATCAGGGGCTTTATTGTTTAGTTTAAACTTAGGTTTGAGTCAAACTGTAAAAAACATTGCTAGTTTACAAGGGGTAAAATTAGAAGTTCACTACCATATCCCTAAACTAGCAGAGGAGATTGAAAACATCTTAAAAGGTCAATTAGATCCTGTTTATGAAGAGATTGAAATAGGTAAAGCGGAAGTTTTACAACTCTGGTTCCACTCTAAAATCGGTAATATTGCAGGAACCATTGTTAAATCAGGAAAGATAAAAAGAGGGAATTTATGTAAGTTATTCAGAGATAAAGAGATTATCTTTGAAGGCAGAATTGACTCTTTAAAAAATGAAAAAACGCCTGTTAATTTAATAGAAACAGGGAAGAATTGTGGGATAGTTATTAATGGTTGCAATGATATTAAGATTGGTGATATCATTGTTGCTTATGAAAAGCAGATAGTTAAAGATGGCAAGCTATAGAAAACAACGGATTGAAAATGATATCATCCGCTTAATTAATCGCACGATTATTAATGAGATCTATGATCCTGTTGTTAAGTTAGGTCATGTTAGCCATGTGAAGTTATCAGCTGATTTTTTTCATGCAGTAGTTTATCTTGATTGTTATGATCGTAGTCAGATTCAAACTGTAGTTAATGCTTTTAAAAAGGCTCAGGGAGTTTTTAGTCAAATGTTAGCACAAAATTTGTACCTAGCTAAAAGTGTAAAACTCCACTTTGTGAAGGATGATGCAATTGACAATGCTTTGAAAATAGAACAGATAATTAACTCTTTGAAAAACTAAAAGCTTGAAGTAAAATTAATCCCAATGGACCCACAAAACAAGTCCCCAAAACCACCAGTTAAATCAACTAGATTGGTTGTCAAAAAACAACCAGCAGGTGTTGTTTTTCCCAAATTGAGTATTCCTGTTAATGATTTTGAAAAAACAGTTACTTTAACAAGAGCACAAAAAAAGGAAGCTAAACTTCTCAAAAAAGCCCAAAGGAAAGCTAATAAGCTTAATAATAAACAAGATAGTATCTTTTTCAATTCTGCTTCAGTAGGTGACACTAATAACACCATTCTTCCTCCTGGTGTTAAAAATCAAGCTGATAATAAAACTAACCGTTTTAGTAAATTTATTAGTTTTTTCACTTCTTCAAAAAACAAACAACCAGATGAAATAACAGAAAGGTTAGTTGATGATCCTACTGTTAAAAACCGTTTTAGTGCTTTTAATAAGAAGCTTATTTGAGTTCTAAAGGATAAAAAACTAAGAGCAAGAGCGTGGAAGATTGTTGGTTATACCAATTTAGTTATTGTGGCATTTTTTGCTGGACTTTTAGCAGTGATGAATAAGTTCATCACCCTTCCCTCAGTTGAATATCCTGCTATTGCTTTACAACTCCCTATTAACAATGCATTATGAGGGATTTCTATCTTTGTTATTAGCATTGTTACTTTACCGTTTTGAACAATGTTTATCTTGTTTTTAATGGGAGTAAAGGATGTGAGAACTTCCCGTTCTATCCATTATTTTATCTGGATAGTGTTAATTATTAATGTAGTTTTATTACTAGTTAGTTGCTTGTTGATGATTGCTGCTTATGCCCATCTTGATGGTTATAACATCTGAAGAAACTTAGAATCACTTAACCCTAATAACTAATGAAATCACTCTTTATTGGTTATTTTGATGGGTTACATCAAGGTCATCTATTTTTAAAGCAGAACAGTAAGTTTGAACCAATGGTGTTATTAATTGATAACCCACCTTTAAAACAAACCAACTGGCTTTATGATTTACAACAACGGGTTGCACAAATAAAAACTTACTTGAAAGCAACTGTAGAAGTATTTGATGTTGCCAAACATAACATGAATGCACTTAGTTTTTTTGAACAACAGATTAAAAGATTGAATTGTGATGAAATTATTGTTGGTACAGATTGGCATTTTGGTAATGATCATAAGGATGGGATCTGGTTAAAGAAACTGTTTAAAAATACTGTTATTGTTAATAAAACAAACCTATCAAGTAGTGTTATCCGTAACTATCTAACTAATAATGAACTTGAAAAAGCTAACCAACTTTTAGTGGAACCTTATTATAGAGTGGGCACAGTAGTACATGGTTTAAAAAAGGCAAGGTTACTTGGTTTTCCAACTGCTAACATTGTTATGGATAACCACTTATTGACTTTAAATAAGGGGAGCTATATAGTAAGAGTTTTATTAAATAACCAAACCTTTTATGGGATTGGTTTTATTAGCCAAAAGGATCAGGATTTGGTGTGTGAAACCCATATCTTTAACTTTAATAATGAGATTTATGGTTCACTGGTCAAATTTACACTGTTAAAGTTCATTAGAACAATTAGTAAGTTTTCCAGTCAAGCAGCTTTGCAAAAAGCAATTCAAAGTGATGCTAACTTTGCTTTAAAGTGGTTGGAAAACCAAAATTTAGATAAAATTTAAAATCATCCAAATATGGACAGTGCCCCCAGTGGTTTAACTTTAACTGTTATTATCCTTAGCATCATTCTGCTTGCTTTTATCAGCACAGTTGTATCAGCTTATGAAACAGCAATCACTTCTTTAACCCCTTACAGGTGAAAGAACTATATCAAGACTAACAACAAGCAAGATAAACTATCAACTAAGATAATAAACCACTTTCAAAACCACTATTCAAGTTGTTTAATTACTATCCTAATTACTAACAACATAGTGGCCATTATGGTTTCTAACATCCTTTTTTTAGCGCTAGAACAAACAATTAAAAATGAGCTTTTATCAAGTGTTTTAAATTTGGTAGTTAGTGGGGTTTTAATCGTCTCTTTTTGTGAAATTCTACCCAAAACTTTGGGCAGAATTAATGTGATTAGAACCCTGGTTCTATTTGCTTATTTGGTTTATTTTTTTTATCTGATCTTTTGACCAATTACTAAGCTAACCAGTTTAATTCTCAAAAAGTATGAAAACCCCTTACCTGTTTCAAGGAAAGATGTTTATTATTTTATTGATGAAATTGAACAAAACGGTTTATTTTCCAAAGAAGATAGTTTACTGATTAAAAAAACCTTAATCTTTGACCAAGTACTAGTTAAAAAGGTAATGATCAAGTGAAAAAAAGTGGCTTATTGTTATCTTAATGACAGTATTAACTTGATTGCCAAGCAGTTTTTACAAGGGCAGTTTTCCAGAATGCCAGTAGTAGATAAAACTACTAATAAGATAGTTGGTTTTATCCATTTAAAGGATTTTTTTACAGCTAAAGAAGCAAACCCTAAGTCACTTGATTTAAACCAGTTGCTTTATCCAGTTGTTTTAGTTCAAGATTCCACCCCCATCAAACAAGCACTAAGACAGATGCGTTTAAACAGAGCACATTTAGCAGTTGTTAATGATAAACATGAAAAAACAATAGGGATTGTTTCTATGGAGGATATTATTGAAGAGTTGGTGGGTGAAATCTATGATGAACATGATGATATCCAACCGATCCAAGTATTGGATGAAAATGTTTGACTTGTTTTACCTAATGTAAAAGCAGCCTACTTTTTTAATAAGTGAATTAAGCCAGATTTGGTAAAATCAAAAAATATTACTATCCAGCATTATCTCGCTTCACTAGATAATGATAGTTTTGCTTGCCAAAATAAACTTGACACCCCCTTATTTAGTGTTGAAGTGATAGCTGATAGCGAAGATAAAACCAAAATTCTTTACGAAATTAGAAAGAAGAGTGATGTTATTGCTTAGAGCATTATTTTTAGAGTTGAAAACCAACAAAAATTGTAAAGCTTTACTTTTGTTATTAATCCCCTTACTAGTTGGTTTAACCTTGATAATCTATGGGATTGTCTTATTTTCCACTGAAGGGGTAATTGATCATGGTGATCATAACCACTTAAGAGCAAGGTTTCAACTCACTTTAGAGGAGATTATTGTTTTTGTTGTTGGTAGTATTATCTTGTTTTTTACTTTAGCTAGCTTTTGTGTGAGTTGCTTTATGTTAATGAGAAGTCCTAAGCAAAAACAGCTAGAGGTTGATCATGCTAATAAAACTAATTTAAAACCAAAAGCAATAGTTAATTGTGATCTTTTTCAGTTGGGTGATTACTGTGTGTTTACATTTAAAAAACTTAGCTTTAAACAACGGTTTAAGCAAGATTTTTTTGCTAGAAGTAAGTTTTCGTTTCGCAGTGAACTGTATCGTCTTTGTTTGGTGGGAGTGTTAATTGCTCTCAATTTAGCGTTAAGTTTGATTGAAATTCCTGGGATAGTTTTACCTTGGGGTAGTTCGATCCAATTCCGTTTTTTTAATACGGCTATCTTGTTTATTGCTGTTAGGTTGGTGGGATTATTATCTACTTCTTTAGTGGCTTTAATCACCCCCTGATTACACCTGTTAATCCATCCTATTCACACCCCCATTAGTAGTTTGTTTTACATGGTTAATGACTTTTTGGTGCTGTGGATCTTTTATTTCTTTTTTTTCCATCTCTTTAAAGCAGAGGTGATCCAAACTACTACTGTAGTTGACAACAAACCTTTTAGTCAGTTAGTTAATACTAAAAAGACGAAGTGAACAAAGTTTTTTTCCTTACTTGTAATTAGCTTTTTGTGTGGCTTTATTGAGGGGTTAGGTTTTTACTTTGGTTATTTTTTAATCCTTGGTAATGTTTCTAGTTTAGGATTGAAGATCTATTATGATGGGTTACAACAACGTGATCTGATTAACAGTAGTAATGTTTTGTTCTTTTTAATGACAACAACTGCTATCTTCAGTATTAAATACATCTTTGAGATGTTATTTTTCTTTAGTGTTGAAAAGAATGTAGTTAATATTGCTAACCACTTTGGTTTGTATTAATCTAAACTTTTTGCAACTAATTAATTAGTTAACTAATAATGAAGTATGTAAAAGTACAGATCATCAATAAATCAACTATTGAACTGTTAGAGGATGCTAAAAAAGGGGAAAAAATTAATTTAGATTTAATTAACCAAGTTGATCAGACCAACATCCTCAATACGATCACTACTAACCAAAAATTAGCATGAGAAAAGGAGTTAAGTGCTCAATTTATCAATCAGCAGAATGAGTTAATTAAAAACTTTGAAATTGAGATCATTAAGTTAAAAACTATGCTTAATGACAAAGAGCAAGCATTGTTATTAAAAACCAAATTAGAATTACAAAACCAGTTTCAAAAGCAGATAGAGAACTATCTCAATGAGATTAACAAGCTCAAGTTAATCAATAAAGAGCTTGAAATTACTAACCAAAAACAACTAGAAAGCTCACTTAAACTACAGCGAAACGAATTTGAAGAGAAAATCAACCAGCAAAACTTAACGATTGAAAAACTGAAAATTCAGCAAGCAAGAAGTAGTATCTGAGCAGTTGCTAAAAAAGGGAATGAACTTGAAAAGTGGTGTGAGAACCAGTATGAGTCTTATGCAGATAGCTTTGAAAACTGTCAGTTTACTAGGTATAAAACTGAAACCAACTTATTAGATGAAAATGATTTTCCTAATGAAAAAGCAGATTACATCTTTAGTTTCTTTGGTAAAAAAAACAATAAAATTCCGTTTTTATCAATTTGCTGTGAGATGAAAAGTGAGTTTAATGATAGTAAGCATAAATCAAAAAACAAGGACCATATTAGCAAACTGGTCAGAGATGCTAAACGTGCTAACTGCAAGTATGCTTTTTTAATTAGTGAACTTGAATTAGAGACTGAAAATGACATCCAAGTACGCTTAATGCCAACATTGGAAAGTGGTGTTGAAGTCTATCTAGTTAGACCAATGTTTTTTATCTTAATGCTTAAACTTTTCTATAAGTTAGCTAAGAAGTTGTTTGCCCTTAACCGTTTTCAATCAGTTGAACTAATTGATAAAAATAAGTTAAATGAACAGTTTAAACAGTTGAAAGATAATTTTTTAACCAAAACCTTTTTAGAGATTGAAAAAGTGTGTAAAAGTAACTTAGTTGATATTGAAACACTTGAAAAAGCAGTGGTGAAACTAAGGGTTAGAAACGAACGTGTTTTAGATCAGTTACTTAATAAATGAACTAAGAAAATTGATAGCTTTGATTTACAGTTAACTAAGAAAATTACTAATAACTACTAGGGTTTAATTTGTTAGCTTATTTAGAAAAATTCAAAATAAGCAAATTATAATTAGGTGTCTTTCCTTACTAAAAATATGAAATTATACCGATCTTTAAAAGCAGCCCTGTTACCAGGGATATGCACTAGCATTTTACTTGCTAGTTGTGCTTCAACCAATACTTATCAAGACCAAAGGAATGCCTTGATTAGTTTGGCTTCTAACCGTGATACTTTAATTGAAAATGCTAAAAAATCTGAAGAGGAAATAAAAAAAGATGTAATGAAAACTAATGATACTTCATCAATGATGACAGCTACCCAAAGTGTTGCAATTACAACACATCAAACTACTGCTCAAAACAGTTCCAAATATGATTTAGACAAGCTTTTTAAGGATTACATCCTTTATGTTGTTGATAATTTTTCAGGACTTGTTTTTAAAAGAACTGGTGGTCATAGGATCCAGTTAATCGATAAGGATAAAGAGATTTTGGATGGTGGTAATCTAACTAAACATACCCACCATGATCATAACCATATGCATGAACATGAACATGAAGAACACCATGATGAAGAAGAGACAGAAGTAGTTGGCAGAGCACTCTCTTTTACTAATGGCATCTTTCTAGTGATTGACTATAAAAAAGACTCTGAAAGAAAAAATATGAGTGGTTCAACTACTATGATGCAACACCACCATGAAGCTGGTGAACATAAAGAAGAGGAAGAACGTAAGCTCTCTTTAAACTTAAAAGCATACAAATTTAATACTCCTTTTAACATTAGTGAGTTTATTAGTGCTTGACATCATAAAGAATCTCATAATAGTAACACAGAGTTCAATAACCTTCACAATAAGTATGGCAAGGAAGAATTGGATATTATTGACTATAACTTTGAAGAAAAAGCTGTTGATGAAACAATTGCTTAACTAAAAAAGATAGTTATTAACTTAATTTAGAATTAACTAATGACAGTGTACACTTACACTGTTTTTTTATTTAATTGAGAAAATATTGGGTTGATGGAACAACAAAACCCTGATCGTTTAAAAAAAGATAAGGAACTTATTTATGCAATTGTTACAGCTAAAGGTATCATTAGCCGTTTCTTTTGATCGATTCTTAGTTTTTTAATTACTAACCTTATCTCCTTTTTTGCAGCTTTTGTAGTGCTCTTAATTTATCTGTTAGCAAGTGTTGATAACCAGTTTGCATTTGCTTTTATTGCTGCAATTATCTTCATTATTTTTTACAACATCTTCTTTTTAAGTTACCTGTTGTTTATCTATTTTAAGGGCCAGAAAGCAATTGAAAACAACTGTAAGTACCTGTTAACAATCCTTGATATTAAGAGTGATGAACTGTTACCTTTTTCGCTTTTAGGTAGTTTAAGAAAAGGTTATATGCTAGATGAAATGCTCTTAGAACAGTAAATATTTGCTACAATCATAACGCTTTAGTTTTTAGTTGATACACCAAAATCCGTAGTCAATTTATTAACTAACTAGTGAACTAGATTTTGATGAATAGCGCTGTAAAATATCCTGAGCTGAAGATCAAACTTGAGTCTTATGATAGCACGCTTTTAGATCTCACTATTAAAAAGATAGTTGAGGTTGTAAAGGGTGTGAACATTAAGATTAAAGGTCCTTTACCTTTACCTACTAAAAAGGAAGTGATCACCATTATCCGCTCTCCCCATGTTGATAAAGCATCCAGAGAGCAGTTTGAAAAAAATACCCACAAGCGTTTAATGATCCTTGTTGATGTTAATCAAGGAGGGATTGATAGTTTAAAAAAGATTAAGATCCCAGTTGGGGTTACACTGCGTTTTTCAAAATAGGTTATGGATGTAAGGGGAATATTTGGTGTTAAAGTAGGGATGAGTCAGATCTTTACTGAACAAAATGAGTGCTTACCTATCACCATTGTTTATTGTGAAGCTAATCAGGTGGCTGGGATTAAAACGATTGCTAAAGATAATTACAACGCCACTCTATTAAGCTTTCAAACTGTTGATGAAAAACAACTTAACAAACCTAAACAAGGGTTCTTTTCCAAACTTAAACTAGCACCTCATAAATATCTGAGGGAAATCAGAAAGATGCAAGGGTTTGAGTTAGGTAAGAAGATCACCCCCCAGGAGTTGTTTAAGATAGGTGAATATGTTGATGTCACTTCACTCACCAAAGGTAGGGGTTTTACAGGAGCGATTAAAAGGTGAAACTTTAAGATAGGTCCTTTGGGCCATGGGGCGGGTTATCCCCACCGCTTTCAGGGTTCAGTGCAAGCAGGTAGAGGTGGTAGTAGTGCGCAGCGTGTTTTTAAGGGTAAGAAGATGTCTGGGCATTATGGTCATGAACAAGTTACGATCCAAAATCTCTTTATTGTTGGCTTTGATGAAATCAATAAGTTAGTGTTAGTTTCAGGCGCAATTGCTGGTCCTGAGGGTGGGATTGTTTTAATTAAAACTGCAAGAAAGAAAACTGGCAAGATAAAAGATATAAAGTTAGCAGTACAAACTGTTAAAGCCCCACAACTAAAAGCACCAAAAAAGCAGAAAACTAAGGTTGAAACCAACCAGGTTAACCCAAAAATTGAAGAAGAGAAAACTAAGTAATGGCTAAGCTTAAAGTAATCCAGTTTGATGGTAGTTTTAAAGGTGAGATCCAACCTGCTAACCACCTCCTTTTAAAAAAAGCAGTGATCCAACCAGTGTTTGATGCTATCTTATTAGAACAAGCAGCATGTAGACAAGGCACTCACTCCACTTTAACTAAGGGTGAAGTTAGTGGTGGGGGTAAAAAACCATATAAACAAAAGCACACTGGTAAAGCTAGACAAGGTTCAATAAGAAACCCCCATTATGTGGGGGGAGGTGTTGTTTTTGGTCCTAAACCCAACCGTAACTACAAACTAAAACTAAACAAAAAGGCTTATCAACTTGCTTTAACTAGTGTATTTGTACAAAAGCTTAATAACAACCAAGTGATAGTTGCTGAAGCCAAGTTGTTTGAACAAACCAATGCCAAAACTAAAAAGATGCTAACGTTTCTTAAGAATGCCAAATTAACTGAGCAAAAACTCTTGTTTGTGATTGATACTATCTCAAAACCACTGTTGTTAAGTACTAGTAACCTAAAGCAGATAGTAGTCAAACAGTTTAATAAAGTATCAGTAAGAGATCTACTTTTAGCTAAAACTATCATCATTGAAAAAGCTGCTTTTACAAAACTGGAGGAACGACTTAAATAGGCTATGGATGTAACCAACATACTCTTAAAACCAGTCTTAACTGAAAAGAGTTATCTCAACCAGATGGGGGAATTGAAAAAATATGTCTTTGCAATTAACCCTAAAGCTACTAAAACTAAAGTAAAACTAGCGTTTGAAATTATCTATGGGGTTAAACCTTTAAAGATTAACACGCTAATTAGAAAGCCAGTGACCATTAGAAATGGCACTAAATACCCTGGGTTTAGTAAGCTAGCAAAACTAGCAGTAATCACCTTACCTAAGGGAATGGATATTGCCATTACTGGTGAGAAAACAACTAAGAAAGAAACAAAGGATCAATAATGGCAATTAAAAAGATTATTAGTCGTTCTAACAGTGGGATTCACAACACCACTGTCATTGACTTTAAAAAACTCCTTACTAATTCCAAACCTGAAAAGTCGCTTTTAGTTACTTTAAAAAAACATGCAGGAAGAAACAACCAAGGCAAGATCACTGTTCGCCACCACGGTGGGAGACATAAACGTAAGTACCGTTTAATTGATTTTAAGCGTTACCACTATGACAATTTAAAAGCAACTGTTAAATCGATTGAATATGATCCTAACCGCAGTTGTTTTATCTCCCTTTTACACTATCAGAATGGGGTTAAAACTTACATCATTAGTCCTGATGGGATTAAGGTTGGTGATCAAGTTTATTCATCTGATCATGCCATTGATATCAAACTGGGTTATTGTATGCCCCTTGCTTTTATCCCTGAAGGAACGCAAGTTCATAACATTGAACTTAACCCTAAGGGTGGGGGTAAGATAGCAAGAAGTGCTGGAAGTTATGCGAGGATCTTGGGTCAAGATGAGACTGGTAAATACATCATTCTCCAGTTAATCTCAGGGGAAACTAGGAAGTTTTTAAAGGAGTGTAGAGCTACAGTTGGTGTTGTCTCTAACTTAGATCATAACCTTGTTGTAATTGGTAAAGCAGGGAGAAGTCGTCATAAGGGAATCAGACCAACGGTTAGAGGTTCAGCAATGAACCCTAATGACCACCCGCATGGGGGTGGGGAAGGGAGAAGCCCAGTTGGCAGAGATGCACCAAGAACCCCTTGGGGCAAACGCCATATGGGTGTGAAAACACGTAACATGAAAAAACATTCAACTAACCTGATTATTAGAAACAGAAAAGGAGAACAATACTAATGTCAAGAAGTAGTAAAAAGGGCGCATTTGTTGATGCTCACCTCTTAAAAAAAGTGATTGAAATGAACAAACAAGCCAAGAAAAAACCAATTAAGACTTGGTCAAGAAGAAGTACTATCTTCCCTGAGTTTGTGGGTAACACCTTCAGTGTGCATAACGGTAAAACCTTTATTAATGTTTATGTTACTGATGATATGGTAGGTCATAAGTTGGGTGAGTTTTCCCCAACTAGAAACTTTAAACAACACACTGCTAACCGTTAGTTATGATTGCTTTTGCTAAACAATACAGAGTTCACATCTCCCCCCAAAAAGCACGGTTAGTGTGCCAGTTAATTGTGGGTAAGAAGATTAATGATGCGCAAAACATCCTTTTAAATACGCCAAAGAAAGCTGCTTACTTTTTAACTAAGTTACTAAATAGTGCGATTAGTAATGCCACTAATAACCACGGGATGAGCGGGGATCTTTTGTATGTATTTGAATGTGTTGCTAACCAAGGACCTAGCATGAAAAGAACAATCGCTAGAGCCAAAGGTTCAGGGAGTGTTTTAACCAAGCGTTCTTCAAACCTAGTTATTAAGTTATCTGATAATCCCAATGAAAGAAAATTACTTTTAACCCAACAAAAGGAACTGGTGAAAAAAAGAACAATGGGTCATAAAAAAGAGAAAGCAAAGCAAAAGCAAAAACAACAATAACTATGGGACAAAAAGTAAATTCAAACGGCTTAAGGTTTGGCATTAATAAGAACTGGATCTCACGGTGAACTGCCAATTCCAACCAACAAACAGCAACCTGATTAGTACAAGATGAGAAGATCCGTAACCTCTTTTTTATCAACTATCGCAACGCTCAAGTGTCTAATGTTGAGATAGAAAGAACCCAAACTACTGTTGATGTTTATGTCTATGCAGCTCAACCTGCTTTATTGATAGGCAGTGAAAATAAAAACATCCAAAAGATTACCAAAATGATCCAAATCATTGTGGGCAGAAAGATTAAACTTGATCTTACTATCAATGAGATCGGCTCTCCGATGCTATCAAGTAGGATCATTGCCCGTGATATTGCTAATGCAATTGAAAACAGAGTACCACTCCGTTCAGCAATGCGCCAAGCTCTAACCAAGGTTTTAAAAGCAGGTGCTAATGGGATTAAGGTATTGGTATCAGGCAGATTAAATGGGGCGGAAATTGCCCGTGACAAGATGTATATTGAGGGCAATATGCCTCTTTCAACTTTAAGAGCAGATATTGACTATGCCTTTGAAAAAGCAAAAACCACCTATGGCATTATTGGGGTGAAAGTATGGATTAACAGGGGGATGATCTATGCAAAGGGTTTAAACAGAACCCCAGCACACATCCTCCATCCCCAAAAGAAACAGCTAAAAACCCCAACTATCAAAAAAACCAATTCAGTAATAGCAAAACAAAAACTCACTGGTAGTGATATTGAAACTGCTAGTTTAAAAGCACTTACTGATAATAATCAAAACCACGAATAGTTAAGATGTTACAACCAAAAAGAACCAAATACAGAAAACCACATAACGTCAGTTATGAAGGACACACTAAGGGCAATGGTTATGTTGCTTTTGGTGAGTATGGAATTGTTGCTACTAAGGGTAATTGGATCGATGCGAGAGCAATTGAATCAGCGCGGGTTGCTATCTCAAAGTGCTTGGGTAAAACTGGAAAGATGTGAATCAGGATCTTCCCCCACATGTCAAAAACCAAAAAACCCTTAGAAGTGAGGATGGGTTCAGGGAAAGGTAACCCTGAATTTTGGGTTGCTGTTGTTAAGAAGGGGACAGTGATGTTTGAAGTTGCTAACATCCCTGAACAACAGATGATCAAAGCCTTAACAAGAGCAGGCCATAAACTCCCTGTTACCTGAAAACTAATGAAAAGAGAGGAGAACAGTTAATGACAATCGCTAAGGAGCTGAAGCAAAAGAGCAACGAAGAGTTAGTGAAACTAGTAATTAAGCTTAAGGGTGAACTCTTAGAATACCGCTTTAAACTTGCCCATGGTGAACTTGACAAACCCCATCTGATTGCCAAGGTAAGAAAGTTATTAGCAGTTGTACTTACTATTCTCACTGAACGCAAAATCAACTGACAAGTTGAAAAAGATAAGTACAAGTTACTTTCAAGAAAAACCAATGAACTTATTGTTAACAGTTGAAAGCAAAAACTATCAACTAAACCTGAATCCAAACAAGAAACTAAAAAGGCTGAAGTTAAACCTAAAGTTGAACCAAAACCTTTAAAACAAGAAACTAAAAAGGTTGAAGAAAACAAGAAACTAAAAAGGCTGAAGTTAAACCTAAAGTTGAACCAAAACCTTTAAAACAAGAAACTAAAAAGGTTGAAGCTAGGATTGAAACTAAGACTAAAGTTGAATCAAAACCTTTAAAACAAGAAGTTAAAAAGGTTGAAGCTAAAAAATCTGTTTCAAAACCCCAAAAACCAGTTAAAGCCAAAGTGATTAAAACAAAGGAGAAAAAACAATAATGAAGCGCAACCAACGTAAGCAGTTAATTGGCACAGTTGTTAGCACCAAAAATGCTAAAACAGCAACTGTAAAAGTAACATCACGCTTTAAACATCCTTTGTATCACAAATCAGTTATTCGTCATAAAAAGTACCATGTCCATAACTTTGGTGAACTTGTTGCTAATGATGGTGATAGGGTACAAATTATTGAAACAAGACCCCTTTCCGCTTTAAAGCGGTGAAGGATTGTCAAAATCATTGAAAGAGCAAAATAGTTTATGGTTAGTTTTATGACAAGATTAAATGTAGCTGATAATACAGGCGCTAAGCAAGTAGGTATTATCAAAGTTTTAGGTGCTACATACAAACGTTATGCATTCCTTGGTGATGTTGTTGTTGTATCAGTTAAAGATGCAATCCCTAATGGCATGGTTAAAAAGGGTCAAGTGTTAAGAGCAGTCATTGTTAGAACCAAAAAGGGACAACAACGCCAAGATGGTACCCACCTAAAGTTCCATGACAATGCTTGTGTGCTTATCAAAGAAGATAAATCCCCAAGGGGAACAAGAATCTTTGGACCAGTTGCTAGAGAGTTGAGAGAAAAAGGTTACAACAAGATTTTAAGCTTGGCGGTGGAGGTTGTTTAATGCAAAGGATTAGAAAAGGTGATAAGGTAGTTGTGATCACTGGTAAAAACAAGGGTGGTAGTGGGATAGTGCTTAAGGTATTAACCAAGCAAAACAAAGCGATTGTTGAGGGGATCAATAAGGTTACTGTTCACAAAAAAGAACAAGTCAACAAGCGCAGCAAACAAACAAACCCAACTACTAAAGAAGCCCCTTTACCATTAAATAAACTTGCTTTATTTGATCAGAAGGCCAAACAGCAAACAATTGGCAAGATCAAATACCAAATTGATCCTAAAACCAAACAAAAAACAAGAGTCTTTAAGAAGACTAATAATGCCATTTAACTGTTATGAATAACCTTGAAAAAACCTATAAAACTGAGTTAGTTAATCAACTCCAACAACAGTTGGGCTTTTCTTCCATTATGCAAGTCCCTAAGTTAACAAAAATCGTTGTTAACATGGGAGTTGGGGATGCAATTAGAGACAACAAGTTCCTTGAATCAGCACTAAATGAACTGCACCTGATTACTGGTCAAAAACCCGTTGCTACTAAAGCTAAGAATGCTATCTCAACTTACAAGTTACGTGCTGGCCAATTAATTGGTTGTAAAGTTACTCTAAGAAATAAAAAGATGTGATCTTTTCTGGAAAAATTAATCTATATTGCTCTGCCCAGAGTAAGGGACTTTCGCGGTTTATCACTGCGCTCTTTTGATGGGAAAGGTAACTATACGATTGGCATTAAAGAACAGATTATCTTCCCTGAAATTGTCTATGATGATATCAAAAGAATTAGGGGTTTTGACATCACTATTGTCACTTCCACCAACAAAGATAGTGAAGCACTTGCTTTACTGAGAGCACTAAAGATGCCGTTTGTAAAAGAATAGATATGGCTAAAAAATCATTAAAAGTAAAACAATCCCGTCCCAATAAGTTTAGTGTACGCGACTACACCAGGTGTTTAAGGTGTGGGCGTGCTAGAGCAGTGTTAAGCCACTTTGGTGTGTGTAGGTTGTGTTTCCGTGAACTTGCTTATGCAGGAGCAATCCCAGGAGTTAAAAAAGCATCATGATAATCAATAAAGTTCCCAAAGCCCATTTTGATCCAGTTTCTGATCTTTTCACTAAGATCAACAATGCTAGAAAAGCTAAGCTTTTAACTGTTACCACCATCGCTTCTAAGTTAAAGATAGCTATCTTAGAGATTTTGATTAAAGAGGGCTATTTAGCTAACTATCAGGTGTTGGAAAATAAAACTAAAACCAAAAAACTAGTTAGTTTCACATTAAAATACACCCAAAGAAGGATATGTTCTATTAATGGGGTGAAACAGATCTCAAAACCAGGATTAAGAATCTATCGTTCCTTTGAAAAACTTCCCCTTGTTTTAAATGGTCTTGGTATTGCAATTATCTCCACTAGTGATGGAGTGATGACTGATAAAGTAGCAAGGTTAAAGAAGATTGGTGGGGAGATTTTAGCTTACGTTTGGTAAAAAATTATGTCAAAAATAGGAAATAGACCAATCAAAATTGATCCTAGTAAAGTGAGTTTAATGCAAACAACAACACTGCTTACTATTAAAGGACCATTAGGGGAAAACACCATTAAACTACCCAAAAACTTACCCTTAAAGTTTGTTGTTGAAAATGACACTATTAAAGTAACTAATAACAACAACTTAAAACAAACTAAGATCTTACACGGTACTTTCAATGCGTTAGTTAACAACGCAGTTATTGGGGTTACCAAGGGTTTTGAAAAGAAACTCATCCTAGTTGGGGTTGGTTATCGTGCTAATGTGGAAGGGCAATTTCTCAACTTACAGTTGGGCTATTCCCATCCTATTAAGGAGTTGATCCCAAACCAACTTACTGTTAAAGTAGAGAAGAACACTGAAATCACCATTAGTGGAATAAAAAAAGAGTTAGTAGGTCAGTTTGCCACTGAAATCAGAAAGTGAAGAAAACCTGAGCCTTATAAGGGTAAAGGGGTACTTTACTTTAACGAAGTAATTGTTAGAAAACAAGGTAAAACTGCAGAGGGCAAGAAATAAGATGACAAGAAACGATAAAAGAAGGATTAGACACAAACGGATTGTCAAAAAGATTAGGTTAACTAACCTTAACAACAGGGTTGTACTAATTGTTATCAAGAGTTTAAAAAACATCTCGGTTCAAGCTTGGGACTTTAGTAAGAACGTTGTTTTAACATCAAGTTCCTCACTTCAACTAAAATTAAAAAATGGCAACAAGGAGAATGCTAAACTAGTGGGAATGGATATTGCAACCAAACTCATCAAACTAAACCAAAAGGATGTGGTTTTTGATACTGGGGGTAGTAAGTACCATGGTAGGATTGCTGCTTTAGCAGAAGGAGCGCGAGCTAAGGGTTTAAATTTTTAAAGCTATGAATGATCAAAAAACTACTAACACTGGCTTGTTAACTTCCACTCTTAAAACCAAGCCCAAACACAACCTTAAACCTTCCAGTGAAGCCATTAAAAAAGCAGTGTCCAAAAAGGAAGGTCATTACAAAAACAAGCGCTTTCAAAAACATAACTTTAATAACAAAAGTGAGTTTGAAGAGAGGATTGTCAAACTCAAACGGATCTCCAAAACCACAAAAGGTGGGAGAAACATGCGCTTTAGTGTCCTTGTTGTTGTTGGTAACAAAAAGGGCAAGGTTGGTTATGGGATTGCTAAGGCATTGGAAGTACCACTTGCCATTAAAAAAGCGATTAAAAAAGCCCATAACTCCATTCATACAGTAGAGATCCATAAGGGTTCAATCTACCACGAAGTGATTGGTAGAAAAGGTGCATCTAAGGTGTTGTTAAAACCTGCACCTTTAGGAACTGGGATCATTGCTGGGGGAGCGATCCGTGCAATTGTAGAGTTAGCTGGTTTTAGTGATATCTATACCAAGAACTTGGGAAGAAACACCCCCATTAACATGATCCATGCCACTATGGATGGGATCTTAAAGCAACTCTCACCCAAAAAAGTGGCATTATTAAGAAATAAACCAATTAGTGATCTATAAAAACAATGGAACTACACCAATTAAAAAGTGTCTCTAAAAGCCGTAACCACAAGTCCAAAGTGGTAGGTAGGGGCCATGGCTCGGGATTAGGTAAAACATCATCACGTGGTCAAAAGGGACAAAAAGCAAGAAAATCAGGTTTAACTAGGTTAGGTTTTGAAGGGGGACAAACACCCCTTTACCGCCGGTTGCCTAAGTATGGGGTTGCTAACAAAGGGATCTTAAAAAAAAGGTGGGTTGTTTTAAATTTGAACAAAGTTGCTAAACTCAATCTCAAAACAGTTACTAGAGCAACTTTGATTGAAAAAAAGGTAATTAGTAAAAAAAATAACCTCCCTTTGAAGTTAATTGGGAACACAAAACTCACTACTCCCATCCACTTTGAAGTGCAAAAAATCTCCAAAAATGCTTTAAATGCAGTGCAAACTAGCAAAGGTAGTGTGAAAATTATCACCTAATGCAAACTGTTTCTTCACCCAAACAAAAACTTAACTTTGGTCAAAGGTTACTAACTCTATTACAGAACCGTGACTTTATGGTGTCACTGGTTTTAACAGTGGTACTTTTAATCTTGTTTAGGGTGTTAGCAATTATCCCCTTACCAGGGATTAGGATTAATGAGAGTGTCTTGGATAGAAATTCCAATGACTTTTTTTCACTTTTTAACTTACTTGGGGGTGGGGGATTAAACCAGCTATCGTTGTTTGCAGTTGGGATCAGTCCTTATATCTCAGCCCAAATCATCATGCAACTGCTTTCAACTGATCTAATTCCTCCACTTTCAAAGCTAGTTAACAGTGGGGAAGTGGGGCGAAGAAAGATTGAGATGATCACAAGAATTATCACCTTACCCTTTGCTTTAGTGCAAGCATTTGCTGTGATCCAAATTGCTACTAATGCAGGCACTGGTTCAAGTCCGATTAGTTTAGCTAATAGTGGCAGTGAGTTTATTGCTTTTTATATTATTGCTATGACTGCAGGGACTTATATGGCAGTGTTTTTGGGTGATACTATCTCCAAAAAAGGGGTTGGTAATGGGATTACTTTGTTAATTCTCTCAGGGATTTTATCCCAACTCCCCCAGGGCTTTATTGCTGCTTACAATGTTTTGAGTGGGATAGTAATTACTCTAACCCCACAGTTAACTGCAGCAATTAGCTTCTTTATCTATTTCTTAGCATTCTTAGTTTTACTGTTTGCCACTACCTTTATCACCCAAGCGACCAGAAAGATTCCCATCCAACAATCAGGACAAGGGTTGGTTAGTGAAGTCAAAACCTTACCTTATTTGCCTATTAAGGTGAATGCTGCTGGGGTGATCCCTGTCATCTTTGCATCCAGTATTATGTCTATCCCTGTGACCATTGCCCAGTTTCAACCCCAAACTGAGTCACGGTGGTTTGTGGAGGATTACCTATCACTTTCAACACCCGTAGGGATCTTTTTATATGCAGTTTTGGTTATCCTTTTTTCCTTTTTTTACAGTTACATCCAGATTAACCCAGAACGGTTAGCTAAGAACTTTGAAAAATCTGGCAGATTTATCCCAGGGATTCGACCGGGCAATGATACAGAGAAACACATTGCGCGGGTGTTAATAAGGATTAACTTTATAGGTGCTCCTTTTTTAACTGTTATTGCTATTATCCCTTACATTGTTTCTTATTTCATTAGGTTACCTAACTCCTTGAGTTTAGGGGGGACGGGGATTATTATTATTGTTACTGCTGTAGTTGAATTTATCAGTGCACTGCGTTCAGCTGCTACTGCTACTAACTACCAACAACTAAGGAGAAACTTAGCAATTGAAGTGCAACAAACAGCTAAACAAGATAGTCTAGAGCAGCTTCAAAAAGAAGCACCAGGGATTGGTAACCTATGGTAGCACAGTTTAATAAGTTCATTATCTTAGGACCCCCAGGGGCAGGAAAAGGTACAGTTTGTAAACTGCTTAGCAAAACAACTAAGTTAGTCCATATTGCTAGTGGTGATCTGTTTAGAGAAGCCATTAAAAACCAGAGTGTTATTGGTAGAAAGATTGCAGCAATTATCAGTCAGGGTGGTTATGTTGATGATACCACTACTAACCAGCTTGTTTATGAATATATCACTACCAATCCATTACCAAATGGTTTTATCTTAGATGGTTATCCAAGAACAGAGAACCAGCTTGATTTTCTAAATATTAAACTAACCATTGACATGGTCTTTGAACTAGTTGTTAGTGATCTGAATAAACTGATTACACGGATTGATAACAGGGTTATTTGTAACAACTGTAACAGTGTTTATAACTTGCTTTTTCAAAAACCACTAGTTGAAAATAGGTGTGATCAGTGTTCAGCTAAACTAGTGAAAAGGAGTGATGATAACAAAGCAGTGGTCAAAGCAAGGATGAAGTTATATCAACAAACAATTCAACCAATCCACACTTACTTTTTCAACAAACAACTTTTAGTACAAATTGATTGCTTTTTACCACTAGAAGAACAACTCAAGACAATCAAACAATTTATTAGATAAAGATGATCTATCTCAAATCTGCAAATGAAGTTGCAGGGATTAAAAAAGCATGTGCAATCTTCAAAGCAGTTAAGGCATATTTTACAATTGAAAAGTTACTTGGCAAAAAGTTGGTTACCATTGATCGTTTAATCAAACAATTCATTGAACAAAAACAAGCTAAATGTGCGTTTCATGGTTATCTAGGTTTCCCTGGTTTTAACTGTCTATCGTTAAACCAAACGGTTATCCATGGGGTTGCAGATCAAACTGTTTTTAAAGATAGTGATAAACTAACGCTTGACATTGGGATAGACTATCATGGTTATCTTTGTGATGCAGCTTTCACTTTACTTGGTAATAAAGCTGATCCAAAGGCAGTAAAACTGTTAAATGATGTTGAACAAGCATTTAGTAAGGTAATTGAACCTGAGCTATTTGTTAACAATCCGATTGGTAATTTATCCAATGCGATCCAAACTTACTTTGAAAACAAGGGCTATTTTCTTGTCAAAGAGTTTGGGGGTCATGGTTGTGGGATTAAGATCCATGAAGATCCTTTAATCTTAAACTGGGGAGAGAAAAACCAGGGCGTTAGGTTACAAGAGGGGATGGTAATCTGTATTGAACCGATGGTTATGACTGATAGTAGTGAGATAACAATGGCAGCTAACAACTGGAATGTACTAACTTTAAAGAGTAAGTTTAACTGTCATGTGGAACAGATGTATCACATCACAAACAACGGCTTTGAATGTTTAACTAACTAATGAAAAACGATAAACTCTTTCTAACAGGTAAGATACTGGAAATTATCCATGGTGATAAGTACCGGGTGATGCTTGAAAACAATGTTGAGGTTGATGCACATCTAGCAGGTAAAATGAAGATGAAAAGAACCAAGATTCTCCCTGGGGATGTTGTTGAGGTGGAATTTTCTCCCTATGATTTGAAACTAGGTAGGATAACCCAAAGAAAATAATTAAAATATTATGAAGGTTAGAGCAAGCGTAAAACCAATTTGTAAAGATTGTAAGATCATCAAACGTCACCGCATCTTAAGGGTGATCTGCAAAACCAAAAAACACAAGCAAAGGCAAGGATAATGGCACGAATCTTAGGGATTGATATCCCCAACCAAAAACGGATCGAGATAGCTTTAACATACATCTTTGGGATTGGTTTGTCAAGTGCAAAAACAATCTTAAAAAAAGCAAAGATTAACCCTGATAAACGCGTTAAAGATCTGAGTGAAGAGGAACTTGTTGCGATTAGAAACGCAGCAAGCGGTTACAAGATTGAGGGTGATTTGAGAAGAGAGATTGCTTTAAACATCAAACACCTAACAGAGATCGGTTCTTGAAAAGGGATTAGACACAGAAAAAACCTGCCAGTAAGAGGACAACGCACTAGAACCAACGCAAGAACCAGAAAAGGCCCTAGAAAAACAGTGGCTAACAAGAAAATTGAAAGTAAGTAATGGCTAAGAAAAAAAAGATTAATGTTCCCAGTGGTTTGATCCATGTCTCCTGTTCACCTAACAATACCATAGTATCAGCCACTGATCCCAGTGGTAATGTCTTGTGCTGAGCGAGCAGTGGTACAGTAGGATTCAAAGGTTTTAGAAAGAAAACCCCTTACTCAGCAGGGGTAGCAGCTGATAAGGTGGCTAAAACTGTGAAAGAGATGGGAATGGGGAGTGTTAAGATGTATCTGAAGGGAACAGGTAGAGGAAAAGACACCACGATTAGAAGCTTTGCTAATGCTGGGATTACGATCACAGAAATCAATGAAAAAACCCCTATTCCCCACAATGGCTGCAAGCCTCCTAAGCGTCCGCGCTAATCAAAACAACAACTTATGGAAAAATTTTTAAAGTACGAAATTAAGGTTAACAACAACCAACCAACCAACACTAACCCTAACTATGGGATCTTTGAAGTAGCACCGTTAGAATCAGGATTTGGGATTACCATTGGTAATGCGATGCGCCGAGTGTTACTTAGTTGTATCCCAGGCGCTAGTGTGTTTGCCATTGCCATTAGTGGGGTAAAACAAGAGTTTAGTAATGTGGAGGGTGTGTTGGAAGATGTGACTGAAATGGTGTTAAACTTCAAGCAACTAGTGGTGAGAATCTCTGATCTTTTGTTTGAAGATGGGGAGATGATCGAACCACCCTTAGAAAGGTGACCAGTTTTAAAAGTTACTGCTGAAAAAAAGGGTGCAGTATATGCAAAGGATCTTGAGTGTCCAGCTGGTTTTGAAGTGATTAATAAGGACCTTTATCTCTTCTCTTTACAAAAGGACATGAAACTAACAGTCAGTGTTTATGTTAAACAGGGTAGGGGCTTTACTAGCTTTCTTGAAAACAGAGAATTGATCAATTCGCTTGGCATTATTGCTACAGATGCTAACTTTTCCCCGGTTTTACACTGTGGTTATGAAGTTCAAGAGGTGAAAACTTCCAAACAAAAGTTAACTGACCATCTCACCTTTAAGATTGCTACTAACGGTGCAATTAAAGCAGTGGATGCGTTTGCTATGGCAGCAAAGATCCTAATTGAACACTTAAACCCAATTGTAAGTGTCAATGAGTCAATTAAGAATTTAACAATTATCCAAGAGAAAGCAGAGGAAAGAAAGGTGAAATCATTTGCCAAGCAAATTGAAGAACTTGACTTTACTGTTAGAACCTTTAACTGTTTGAAAAGAAGTGGGATCCACACACTCCAAGAGTTACTATCAAAGTCATTAACTGACATTAGAGAGATTAGAAACCTAGGTAAGAAATCAGAACGGGAGATTATCAAAAAGGTGCAAGAGTTAGGTTTAAAACTCCGTTCTTAATAAAATAGAGCTATGTCATACATTAATAAAGAGGGGAAAACCACAGCTTGAAGAGTGATGACAGTGCGTCAGCAAGTGAGTGCAGTGTTAAGTTATGGGAAGATTCAAACCACTTTAAAAAAAGCTAAGAACACCCAAAAAAGGTTAGAGAAGATTATTACCATTGCTAAAGTTGATAACTTTAACAACCGTAGGGCTGTTAAAAAGTGGTTATTAAATACCAATTCATTGGATGTAGATCAACTCACTAACCACCTTTTTAAAAAAGTAGCACCACGTTTTTTAAAGCGTAATGGTGGTTATAGTAGAGTGTTAAAGTTGGGAGTTAGAAGGGGTGATAGTACTGAAATGGTGATCTTACAGCTGATAGATGCTACCAACTAAACAAGCTGCTTGTAGTTTTATTAATGTTGCTTTTTCCTATAATGAACTGCCATTAATTAGGGAACTATCTTTTAGTGTTTATGAGGGGGAATATGTTTGTATTGTTGGTCATAATGGCAGTGGTAAATCAACCATTTCCAAACTGTTAACCGGGTTATTAAAACCCCAGTCAGGTGAGATTAAGATCTTTGGTAAAACAGTTGATTTTGATAATGTTAGTTACTTGAGAAATAACATTGGGATCATCTTTCAAAACCCTGATAACCAGTTTATTGGGATCACTGTTGAAGATGACATTGCCTTTGGGCTTGAAAACAAGTGTTTTTCAAGACAGAAGATAAAAGCCATTATTGATGAAGTGACATTACAAACCCAAACTGATGGGTTTATTAAACAAGAACCCCATAACCTATCAGGCGGACAAAAACAACGGGTAGCAATTGCATCTGTTTTAGCACTAAATCCTGCTATTATCATCTTTGATGAATCAACTGCGATGTTAGATCCTAAAGCTAAAAAAACGATTAAGCAGTTTATGGTTAAACTATCTAAACAGGGCAAGTGTGTGATCTCAATTACCCATGATATGGAAGAAGTTACTAAAGCTGATAAGGTGTTAGTAATGAATGAGGGCAAACTGATCAAACAAGGCAAACCTGTTGAAGTTTTCACTAGTGAACAAGAGTTACAAAAAATCCGTTTAGACATCCCTTTTTCACTCAGTCTTTCAACCAAGATAAAAGGGATCACTAGTACAATTGATTACCAAACCCTGATTAAATCAATTGCCAAGCTGTGAAAAAAAAGATAGTCCCAATTAACCCTTTAAAAGCAGATGAGATTTTAGCAGTTAGTCACTTATCATGTGTTTTTAACAGTAAAACTAACAATCCCATTAAGGTGATTGATGATTTTTCCTATACCTTTCAAAAGAACCAAATTTACTGTATTATTGGTGATAGTGGCAGTGGTAAATCAACCCTTGTTAACCACTTCAATGGGTTGATAAAACCCAACCAAGGTGATATTTGGGTTAAAGATATCTATATTGGTGCTAAACAACGCAAGATTAAGAACTTTAAAAAACTGCGAAAAACTATCTCAATTGTTTTCCAGTTTCCTGAGTACCAATTGTTTAAAGATACCGTGGAAAAAGACATTATGTTTGGTCCAGTAGCATTAGGTCAATCCAAGTATGATGCGCGCCAAAAAGCGGCTTATTATCTGGAGATGATGGGGTTAAAATACCCCTTTTTAGAACGTAATCCCTTTGAATTGAGTGGGGGGCAGAAAAGAAGGGTAGCGATTGCTGGTATACTTGCAATTGAACCAGAAATTCTAATCTTTGATGAACCAACTGCTGGGCTTGATCCTGAAGGGGAAAGGGAGATGATGCAGTTAATTAAAACTGCCAAACAACAACAAAGAACGGTATTTATGATCACCCACCAGATGGAAAATGTCCTTGAGGTGGCTGATGTGGTTTTGGTTTTAGCTAAGGGTAAACTAGTAAAAGCTGCTAGTCCATATGAAGTGTTTATGGACCAAACTTTCCTTGAAAAAACAACGATTGTTCTCCCCCCTGTGATCCAAGTGATCAAAGATCTAATTGCGATTAATGCTCACTTTAATAAGTTAATTGAGTTGCAACCAAAGAACCTAGAACAGCTTGCATCAGCAATTAACAAGACTATAGCAAACCATGGATAACTTTATTAATGGCTATATCCCAAGAAACAGCTTTGTTCACAAGCTGCATCCAACTACTAAACTAGTAATCTTTTTACTGTTAGTTATCTTGGTATTTGTACCAATTGGCTTTGTTTTTCAAAGTGTTATCTTTCTTTTTGTTACCTTTGTTTTTTTTATTGCTAAACTCCCGGGGCGGTTTTACAGTTCAGCAATTAAGTCAATTACGCTGTTATTTCTCTTGTTGTTATTTGTAAACTGGTTTACCTTTCGTGATCCAGGGTTTTATCTTACTAGTGATCAACTTAACAGTTTACCAGCCATTGATAACAGCAAGTTTAGCTTTTGAAACATTAGTTTGTTTAACTATCAAGATAATGTTTTTTCCCAGGTTTTTGCTTTTAACAGGGGTAATTTAACCAACTTAAATCAACTTGATTTTTTCTATAAAGCTAACAATGCTGATAGTTACACCAAAGTAAAGGGCATTGATAGTTTAGCAAGTATGCTAGCAAACAATGGCAATGGTTTAAGCAAAGACAAAATTCTGAGTGCTTTTTTAGATCACAATTTAAACCTTTATTTAGCGAGAAGTTGGGGGGCAAATTTTGCTGGGTTTGTTGTTGATTTTAACCCAACAACCCAACTCTTTAAACTCACTCCTTTTCTAGCAAATGCTAGTTATGTTTTAACGTTAAGAGCAGTTATCTTAGCATTCTATGTCACCCAAAAGATCCTAATTATGATCTTATTTGCAACTGTACTCACTTCCACTTCAAGTTCAGTTGAACTAGCATATGGGATTGAAAGGTTATTATGACCTTTAAAACTCATTAAAATACCTGTTAATGTCTTTGCAATGACCATTGCCATTGCCATTAGGTTTGTCCCTTCCTTGTTACTAGAATCACAACGGATCTTAAATGCCCAAGCATCCAGGGGTTTAGACTTTCGAAATGGGGGATTTTTAGTGAAGATGCGTTCACTCTCTTCGTTAGTAGTACCAATGGTCTCCATTGCATTTAGGAATGCCTCTGAACTTGCTAGTGCAATGGAAGCAAGGGGTTATCACCCTGCAAAGAAGCGCAGTAGTTATAGACAATACAAAATCACTTGGATTGATATTTTAGCGTTATTTTTGGTTTTTGCTTGGTTTGTTGTGATTATCTTTTTAACTATTAGAGGAGCGGTCTTTTTGGATCTAGGTACACCAGAATGGTTACTAACAGGAAAGATTAATGAACAGGTAATCAGGGATCTGAAGGTAAGTGGCTAGGTACTTGGGGATTGTTAGTTATGATGGCAGTTACTTTAAAGGGTGAGCGATTCAACCAAACCTAGCTACTATCCAAGATTTATTGGAACAAAGTTTTTCATTAATCATTGGCAGAAAGATAAAGGTGATTGGTTCAGGTAGAACTGATAAAGGGGTACATGCCATCAACCAAACCTTTCATGTTGATATTAATGGTGAAATTAATCTCAATTTGTTAATTAGAAAAATTAACCAGTTGATTAAGCCCCACTGTATAGTTAAAACCTTGGTATTGGTTAACGATAGCTTTCATGCGCGGTTTCAAGTTAAAACCAAGGTGTATGAATATCTGATTAACTGTGGGAATTTAAACCCGTTGCAATTTAACTATGTTTGGCAGTTAAACCAGCAATTGGATCTTGAAAAACTCAAAGCTGATGCCACTTTATTTTTAGGTAAGAAAAACTTTCTTAGCTTCAGTAGTTCGATTCACACTGATTCAATTCGCACAATTAGTAAAATTACCATACAAAAAGAAACTAACCAACTAGTTAGACTAACTTTTTTTGGCAGTGGTTTTCTCAGGAGTCAAGTGAGGATGATAGTTGCTTGTTTAGTGAATTTAAACACTAATAAAATGGCACTTGAAACAGTTGCAAAATTGTTTGAACACCCCAAGAAAGGGAGTTGTGTTGTTAAAGCCCCTAGTTGTGGTTTGTATCTGAAAACAGTGGTATATGAAAAATAGTTACAAGTGGGATCTATCAGTTTTATTAAACAACCAAAGCTTACAAGCAAATTTTTTAAAAATTCAAACAGTTAGTGAAGCGTTAATTAAAGCTTATAACAACGGGTTGTGTTTTACAAATAAAACTAGCTTTGAACAGTTTTTAGCAATCGATGATAAGTTCACTGAACTTGAAAATCGTTACACTAACTACCTTTATAACAAGCAGAATGAAAATAACTTGGATAAGGAGGTTAATGATGCAATTTTTGCATACCAGAGTTTTAAAAATAACCATAACCTTGCTTTCAGTACACTGCAACAGGAGTTATATAACCATGAAAAACTCATTAAGGATTATTTAACTGATCCAAAGCTAGCGGTTTACAAGCGCAACTTAATGTTAGTTTTTCGCGATAAACCCCACCAACTATCTAGTCAAACCCAGAGTTTATTGAGTCAAATTAACCCTTGTTTTAACCAAGCAGAACGGATTTTTAACATCCTTTCAACTGCTGATTTAAACTTGCAACCTGTTGTTTATCAAAACAAAAAATATCCGATTAACAGTGTGAGTGATTATCAGTCCTTACTTGAAAACACTAACAGGGGGATTAGAAAAGCTTGTTATGAGAAGTGGATTGAAATTTATTGAACTAATAGAAACAGCTTAAGTTTAAGTTTGGTGGAAAATTACATCCAACTAGAGAACTTCGCTAAACTAAAGAACCATCCTAGTTACATTGCCCAAACTGCTTTTAATGATGAGATTGAAGTTGGTTTTATTGATTTTGTTTACCAACAAGTAGCTCAGTTTGCCAAAACCTTTCAAGCATTTATTCGCTTAAAAAAACAGATTTATAAACATGTTTTAAAAGTCAATAAAGTTGAACCATATGATCTTACCCTAACACTTTTTAAAACTAAAAAACCATACACGATTGAACAAGCTAAACAAGATGCACTAAAAGTTTTAGATCTACTTGGTGACAACTACATCAAGATAGTTAAAAAGGCTTTTAATGAAAACTGGATTGATTGACTAGCAGATAAAAACAAGTATACAGGGGCATATTCAATCTCCAATGTTAAGGGCTTAGAGCACTTTTTTATCTTAATGAACTTTGATAAAACCAAATCATCACTAAATACGTTGGTACATGAACTTGGTCATTCAGTTCACTCTTGGTATGCATCACAACACCAATCCCAAAACATCGATCCTACTATCTTTTATGCTGAGATTGCTTCTATTGCCAATGAACTGTTGTTGTGTTATTATGAACTGCAACTTTATAAAAATAACCACAAGCAGTTAATTGCTAGTTTATTGAGTCAAATTAACCATTTTTTTGGCGCTACTACAAGACAAATAATGTTTTCACAATTTGAAAAAGATACGCTTGATTTAATCAGAGTTAACCAGAAACCTGACTTTAAAACTTTGATTAAAATTTATGCAAATACTGCGGTTAAATACCAAGGTTTTAAACCTGAAGTAGTTGCTAATAAACTAAAAAAGACCCAGTATCAAAAATCATTGTCACACATCATTGCTATCCCCCATTTTTATGCAGGTAACTTCTATGTTTACAAGTATGCCATTGGCCAAGTTGCAGGTATTTTAGTAGCTAAAAAAATTAATAGTGGTGATAAAAAGATGAAAGATAATTACTTTAAATTCCTCAGTTCAGGTTCTAGTTTAGCGCCACTTGAAACCATTAAACTCTTAGGGATTGACCTTACTTCACCCCAAGCCTGACAAGAAGCACATAACGAAGTAAAGCGTTGGCTTAAAATTGTGAAACAAAGCTTTAAAAAACTCCAAAAATAAGTGCACCATTTTAACCGCGCTAAAAAGGCCAAGAATAACGAGTTTTTTACTTTAATTGATGAGATTGAAAACGAAGTAATTAACTACCAAAAGCAGTTTGCAAATAAAACCATTTTTTGTAACTGTAATGATGGTAAAAATTCCCATTTTTTTCAGTTTTTTCAAACTAACTTTAACCAGTTACAACTAAAAAAACTCATTGGGTTTAGTTTTAATAATCTCTCACAAGCTGACAAGTTCACTTTTGATGGAAATAAAGTAACTAAAACCAAATTAAAGGGTAATGGTGATTTTAGTTCTGATGAATCGATTGAAGTGTTAAAACAAGCAGATATAGTTGTAACAAATCCACCCTTTAGTTTGTTTCAAAGTTTCATTGATCTGTTAATACAACACAACAAGCAGTTTCTGGTTTTAGGGTTAAATGCAGCAGTTAGCTATAACCATATTTTTACCTACTTTAAAACTAACAAGTTGTGGTTTGGCTATACCGTTAATAAAACAATGAGTTTTTCAGTTAACAGTGACTATCAACTTTATAACCCCAAAACTAGTAACTTTTTTACAAAAAATGGCAAGTGTTTCCAAAAGATAGCAGGTATCTCTTGGTTTACTAATTTAGGAAAACCACATTATAACCCCTTTTTAAATACCAACTGTTTTTATAAAAACAACGAAAAAAACTATCCCAAGTTTGATTGGTATGATGCTATTTATGTCAACAAGATTAAAAACATCCCTATGGATTGAAATGGATTGATGGGAGTTCCTTTAACCTTTTTAAACTGTTACAACCCCAAGCAGTTTGAATTAGTTGATTGTCTTGCTAACCCTTATGCTACCTTAGATACATTAAAAACAAATGCCTTTGTGAAATTAAATCAGGGTGATGTGAGAAATGTTAATGGTAAAAGAAGGTATGTAAGGGTAATAATTAAAAAACAACAAATTTAGTTTTTTCAACATTTAACAACTCTGTTTTTACAAAAGTTAGCTTAATTTTGCTAAATTGTTTTTTGATAGCACACTGCTGTCAACGAACCATGTTGATAAATGAAATTCAAACTATTTTTTTTATCAAGCAGTGTGTTGGGTCCAATTGCCCTATTTACAACTGCTTGTAGTGCTGTTTATAGGTTTGATCAAGTTGATGATGGCAAGATTAAACTAGCAACTGTAACTTCAGCTTCCGCTAGTGGCTCGCTTACTACTATCATCAGTAAATATAATTCACAAAAAGATCCTAATGATTATCCAGTGGAACTGGTTTCACTTGATAGTAGGGGCAGTTATTCTAATGGCAAAAAGGATCTGCAAGCTAAACTGCTAGCTAAGGATAAAAATAACTTTTATAACCTTACTTTTAACTATAGTGATGTAGTTTCAATCCTCTCAAGAAGCCAGATGGAGTTGAGCTTTGATACGGTTGATACTAGTAATTTTGATCCTAGTTTTCTTAGTTTTAACAATAACATTTCCAATGTTAATCCAAATAGCATCTATGCTTTACCTGCTACTGTATCAGGTGAAGTTTTAGTTTTAAATGGACCGGTGTTACATTACATTTTAAGTAGTGCTAAAAAAGATAGTAACACCACCCTTTCAACCCATTCAGCTAGCAATAATAGCAATAAGGGAACAATGGTTGTTGCTAGTGATAGTGAAACATCAAGTTTATGAACTAAATTAGAAGCTGCAGCAAAAATGAATGCTCAAACTAATGAAACCCAAGTTTTAAAAAGTAATTCATCAGAATCTAACCAAACCCAAGCTAGTGATACAGAGATTAAAAAGATTTGGGGTGATTATCAAGAAGTTGATGGAGGGTTGAAAAATTACACCTTTAAAGCTAGTGTTTTTAACAATTGAAAAGACCTAAATGACTTTGCCACCAGGATTGCAAAATCCTTTACAAAACTGCAAACTACCACTAAAAAAGGGGAGGAAGTACAAGCTGTATTTGGGATTGGTAGTTTGGAAAATGCCTTATATACTGCTTTATTTGCTTCTGGAAAAGCTGATTACAATAACTTTCTTTTTAACATCAAAAACCAGCGAATTAATTTCAGTAACTTTTTTAATAAATCCTCAACTGCATTTCAAAACCTTAAAACTATCTTTAACAGCTTCAAATCCTTAATTGATCAAAACGGTTTAATCTCAAATGCACACTTTAACACCCCAGTTAATGACTATGCTAAGTTTAACCAATTAGCTTTTTACACCTCTTCAACTGCACGCTTTCCCTATTCATTTGCAAGTGATAGTGTAAAGCGTTTAATAGTTAATGACAAGACAATTGAAAACAAAAACAATAAGAGTGTTTTTGAGGTTAATTTAAGTAGTGATAGTGATAACAACAGTAACCTAATTGGTACTGTATCACTGGAAAATAGTAAACAAGTTTCACTCTATGAAAAGCAAGTGGATAGTAATAAACAAATTGGTGTTGATGCTTTGTTAATCAAAGATGAAACTTTAATCAACCATCTTAAGAGTTTAAAATCGCAAGTTAGTGCAAAAAGTGCTAGTGAAACTTCCCAAACAAAACAAAACAAAACCTTTTTAGCATTTACAACTGTGAATGCAGATCAAAAAGCAATCTTTGATGTTGGTAAACTTAATGGCAAAACTGCCAAAATCATTATTAATGCTACTGAAACAACTAATGCTAAGATTAGCACCTTACAAGAAAAAGAGGCAATAGTTCTAAAAGCACCCCAACGCTTTGAGAGCACTGATCCATTTCCTATTGCTTTAGTGCAAGGTCCTAGTTTAATAGGGATCCATGCAAATGAAAGAGAGGATATTGAAACCAAAAAGTTTGTGAATTGGTATCTGAATACTAAAGTCCAATGGAAAGAAAATTCTATTAAAACTCCTGCAGAATATGTAGCTGATAAAGCTTCTTATCTTTTACCTTTTAAAAATAGGCTAAATAATACTAACAGTTACAATGAGTTTGTTAAAACTGCAGTTAGTCAGTTTGCTGATAAAAATGTAACTAAATTTGCAGAACCTGCTGATTTTTTATCCAACAAAGTACGTGATGGTGTTAAGAGTAATTTAAATGCTGCAATTAACAACCACAGCATTGATTTTGATAGTTTTATCAATGATTTAACAGACTATCTTGGTAGTGATGTTAAAAACATCTAATAATTTATTCCCCCAACCTCTATTTTGTGTTACTAGTGCCTAAGGTGGTAGTGGAGTACCACCAACTTGATAAGAAGGTTGTCAAAGAGAGTTTGGAAGTGGAAGCAACTTCCACCTTCAACCCCACCCAAAGGTTGAAGAGTGATAGTCCAATGAAGGATTCAACAAAAACAGGGGAGAAACTCCAAGAGACCATGTCATCAATGAGTGGTATGGCTACATCCACTCGCAAGGCCCTCAAAGTGGAGGTGGAAAGGGGGAGTAATGTCAATCAAGGCGAACTAGCAAAAAACGACTTTGCTAAAAAGCCACTAAAGCATAAGAACAGTAGTGGGACAGATGTGAAGTTAGATTCACAGAAGGATTTTACTGAAGGAAAGGTTTGAAAACCGGTGTTGACTACGGATGAGATAAAAAAAAATAGGGGGATGGGGGCTTTTGCCAACTCCCCCTCCCCTTCAACTTCTGCTTCTTCTCTTCCCCACTCCCCACTTTTTCTAACATCAATGTTGGGGTTAAATCAATGATCACTCAACACTTAAACAAAGAAAACACCCGGTGGGTGTTTACCCCTAACTCTTCACCAGACATTTGAACGGGAGCAGGGTATCGCAAGGGTTCATCAAACACCAACGGCATCCCTTTTGAACAGGTGAAGCCTAGCAATAGTAGTAGTAGCAACACGTTTAATCCCAATGATGATGAAAATCAAGTCACTCAAGGTGGCTCCTCCAAAAAAACAACGTATGACAATTTACCAAACTCCATCAGTCCCACCAGTGACTGGATCAACGCATTAACCTTCACCAATAAGAACAATCCACAACGCAATCAGTTGTTGCTCAGAAGCTTACTAGGAACGATCCCGGTATTGATCAATAAGAGTGGGGAGGGTGGGAATGAGTTTAACCATACGAATGATCAGAAGTGGGATAAAACGAATGAAAAAGATGGGAATTTACCTGGGTTTGGGGAGGTGAATGGTGGTTTTATTAAGTTTTTATCAAATTTTTATTAGTTTATTAGTTAAAAAAGTCGCAATTTTTATTAGGTTTTTATTTATTTTTTAGTTAAGAAAATGTTAGGTTTTTATAAGTTTATTGGACAAAAAATTTCAAATTTTCTTAATTTATTTAATAGTTAAAAAAGCGTTAGGTTTTTCTTAGTTTTTTATTTGCTTAATGGTTAATAAAAGCGTTAAGTTTTATCCTATTTTTAATTAATAAAACCTCGACCCCTATTGCCAAACCATCCATAACACCAACTTGTTTGTGTTGTTCAAGTCTACTCAGGTGAAAGTTAAGTATGAGTTCAAGTGGCAGTAACAACACAATTAGTTTTGAAAACCAAGGTGAAAAACCATCCTACATCGTTGAGTTTACTAATTCTACCAACATAGGGATCAAGTGAAGCGTGGTGAAAAAATACCAGTTGGATGTACCGAATGTTACCAATGAGATGAACGATGTACTGAAAGAGTTGATCCTTGAACAACCTTTGACTAAATATACCTTAAATTCGAGTTTGGCTAAACAGAAGGGTAAGACACAAGCAGTGGTGCATCTCTCAAATTCAAATCAGTGACACTCGATGCGTAATCAACATGACCTAAACAACAATCCCTCTCCAAATGCTTCAACCGGATTTAAATTAGACAAAGGCAACGCATATAGAAAACTAAGTGAATCCTGACCAATCTACCAACCAATTGATGGGACCAAGCAGGGCAAAGGGAAGGATAGTAGTGGGTGGAGTTCAACTGAAGAAACAACGGCAGCAGGGGATGCGCCCAGTGTTACTGGAGGGGGAACATCAGACACCGCTTCAAAATTTAAAAGTTACCTCAACACCAAACAAGCGTTAGAAAGGATCGGTATCTTGTTTGATGGAGCAATGGCGAGGAATGTGGTTACCCTCCTTCCTCTACTTTCAACCCAACAAGGTGAAAAGTGGTCAATATCAACAAAATAACACCTACAACAGGTTAATTGAACCGGAAAGTGCAACAAGTGCAGCAACTAACATGACCAACTTGTTAAAGCTGTTGTCTAGTAAAAACATCAAACAAAAGTTGGGGAAGGGGGACACCCAATCAATGGGAAATAATGGAGTGTCCAAGACACCTTCTCATTTGTTGTTCCTTATTCGGGGAATCATGACAATAATGGAACAACTGGACCCATTAAAACTGCTTATCCAGTGAAAAACACAGAGAAAGCAACTGTCAAGATCAATTCCTTGATCAACGCTACGCCGTTGAATAGTTATGGGGATTTAAATAATCACACCAAGTTAATTCACCCAAATTATTTACTTATTTATTAACCATTGTTACCCAATTTTTCAACCACTTCCCCACTCTATAAATTGTTATTTTTTAACAATTTGAACAATTAAATTGCAAAAAATAACTATATTAATGTAAATTTAGCTAACAAGCTTAACTGGTTGTTTTTGAGATGAAAGGGTTTTTAAAACCAAATTTCTCGCTCGGTGCTTTGTTTTTAACTTTAAGCCCCATAGCCACTGCATGCATTGCTGAAAAACCAGTTAACAACCGCTTTAACTTTAATAGCGAGCAATTAGCTAGGCTAAGAAAAGCAAGGGTTAACCACTGAAGAGATGGGGATACTTTGGAAGTTAGCTTTGCAAATAACCACCAAAAACCGATCCGTATCTATGCCATTGATACCCCTGAAAAAGCAGTTTTATCTATAAAACGCAAATCAGAGATAGAACTTAAAGAAGCTAATAAAGCAACTGAGTTTGCCAAAAGCTTAATTCCCATTGGTAGTGAGGTGTGGATCTGACCACTAAATAGCTATAGCTATGATCGTGAAGTAGCTGCAGTGTTTTTCAAAACCAATCCATTGCAACTGCACTTTGAATCGTTTGCAGTTGAAATGGTAGCAAATGGTCATGCTTTACCTATTGCTGGTAATGACTTTGATTTTGTTTTTAGTGATTTGGACCCATTTAATCCCCTAAAAATAGTAGGGATTGAACTAGCTAATGGTTTAAACAATGCTTTTAACAACAGAAAAAACATCTTTAGTTATTTAGAAAACAGTTTTCAATCAATAACAATGGTCTATCAACAACGCGGTGTTGACCAAAGTTGAACAAGGTATTTAGCTCCTAGTAATGATTTTTCCTCTACTAAACTGGGGTTGGGATTAACCATCTATGAATTGAAACTAAACAATGGCTAACAATAAGAGTGCAATTGAGTTGAAAAACATCGTTGTTGATTTTGGTGAATCAGTTGCGATTGACAACATTAACCTTAGTGTTGAAAAACACCAACTAGTTAGCTTACTTGGTCCTAGTGGTTGTGGTAAAACCACTACACTTGCAGTTATTGCAGGACTTATTAAACCAACTAGTGGTCAGGTGTTATTTAATGGTTATGATGTCACCAAAAAACCACCCCAAGAACGTAAACTAGGGCTAGTTTTTCAAAACTATGCACTTTATCCGCACATGAATGTGTTTGAAAACATTGTTTTCCCTCTCTACAGTGATAACTCGTGAAAACAAGCAGTTTTGGAAAAAAACAGTGTTGCAAACCATGAGATTAACTGTTTGTTACTTACTAGCAACGGTGCATCAGTTCAAGAGATTGATCAGCTCAATAAGTTATTTCATGATAGTATTGAAAAACCCAAACAGATCCAATACCAAATTAATGACCTTAATGTTAGTGTTTTTAAAAACTTAAATGAACTAACTGCAAACCTTAAGTTAATACCAAGTAAGCACCAGTTTGCTATTACCAATCTCAACAAACAAACTCTAAAACAGATTAATGAACTGGAAGCTGAGTTTAAAACAAAGTGAAAGTTACAAAAACAAACCCCAATTAAGAGTGGGGTTGAACACAATGCCAAACTCCAAGCAATTAAACAACACTTTAGTTATGAAAAACAACGGTTAAAAAAACACTATTTCAAAACTAAAGTGGAACTAAAACAAACCCTTGTTGAAAACCTTAAGTTAGTTAAAAAAGCGATTAGTGAACAAACTAAGTTAATTAAACAGAGTAGTGATTACACTAAGTTAAAGCAATTAAAACGGTTAATTAAAGTTGAACCTAACCAACTCAAAAAACAATATAAGGTTTTTCTCAATCAGTTAATTAAAAACTATTCACTTAAAACTGATAAGTTAACTGATACTCAACTTAATGAAATTGAACAGATTAAAACCAGAATTGTTTCAATAAAACAGTTTATCAACAAAACTGCACTTGAAGTTGCTAACAAACTAGCGATTACCAAGATTTTAACCAAACGCCCTGATAAGATTTCTGGTGGACAACAACAACGCGTAGCAATTGCTAGAGCAATTGTCAGAAGACCTAAACTATTGTTAATGGATGAACCACTCTCTAACTTAGATGCAAAGCTAAGGGTACAGACAAGACAGTGGATCAGACAGTTTCAACAGGAGTTACAAATTACCACTGTTTTTGTCACCCATGACCAGGAAGAAGCGATGAGTATTAGTGATGTCATTGTTTGTATGTCAACTGGAAAAGTGCAGCAAATCGGCACACCCAGTGAACTTTATTTAAAACCTGCTAATGAGTTTGTTGCGCGCTTTTTAGGCACCCCTGAGATGAACATCATTGAATGTAGTGTCAAAAACAACCAGTTGTTTTGAAACAACCATCTGTTAGTTACTGAGAGTTTTAAGCTTAATGTAGAGAAACTCTTAGTTGGGTTTAGGTATGAACAACTAGTGGTCACTACTAACAAAAGTAGTTTGCAAGCTAAACTAATTAACATTGAAAACTTAGGTAAACACTTAGTTGCTACCATTAGTTTGTTTGATACCACCTTATCAATGCGCTTAGAATTGAATAGCCACTTAAAAGTAGGTGATAGTTTAAATTTCATTATTAAAGCTAACAACCTCCATTTTTTTGATATTGATACAAAACAACGGATTGAGATTTAACAATGTTTAAGTGGTTATTAAAACATCATAATCAACCTCATAGCCTCCAGTTAGGATTACTAGACCAACCATTACCGTTCTGAAAGCCCTTTTTGTTGTTCCTCCCTGCGCTTTTAACAACAATTTTGTTTACCATTATCCCCTTCTTTTTAAGCTTACAGAAGGGTTTTAGTGCTAACAGTGATCTGTATGATCTCTCCTCCCAATTCTTTAGTTTACGAACCTTTCAGGATCTGTTTAGTGAATCTAACTTTGTGTTGGGCTTACGTAACAGTTTTCTCTATTCACTCATCTCTTTACCCTTTAGCATTATTATTGCTATTGTTATTGCCAGTGCCATAGTATTTGTGTACAAAAAATTGTTAAGAGGGTTTTGACAGACCGTGTTTTTTTTACCCTATGTAACTTCAGGGGTGGCAATCTCGATTGCCTTTGTATATATCTTTGATTCTGCCTCTGGTATTTTAAACACGGTGTTTAATGTCAACACCAAGTGGCTTGATTCAGGTTCACGTGATACATTTAATGCCTTGTGGGCTATCTTAATCTTTGGGGTGTGGAAAAACTTGGCATTTAATGTGTTGATTATCTCAACAGCAATGTTAAGTGTTAATCCCCAACTTTACAAGGTAGCAAGTTTAGATAGTGCCAATCCTGTAAGACAGTTCTTTAAGATCACTCTTCCCTCCATCCGTCCTACTTTAATCTTTCTTACTACCCTTTTAATCTTAGGGGGGATGCAAGTCTTTCCGCTGGCTTTGTTTGAAAACAAACCTGAAGAAGCGGTTGCTAATGGGGGGAATAGTATCTTGCTTTACATCTTTCAACAGATCCAAAGTGGCAATACTAACTTAGCAGGTGCTGCTACTTTAGTGTTGTTTGTGTTGGGAGTTTGTTATGGGTTAGTGTTACGTAATGGCTTTTATCTGATTGAATGGTTGCAGTGAAAAATTAAACAACTTTATGTTCAAAAACAACTTACGCTTTACTAGTTGAATTAATCAACACAAGTTTTACCAACTTGATCTGAGCTTAAAAACCCGCTCAATCAAACAAATTGTTTTAACGTTGGTTTTCAAAACATTGGTATTGGGGTTTTTTGGGTTAATTGTCATCTTTCCCTTTTATCTGATGGTTGTGGTTAGTTTTGCTAGTGATGAAAGAGCATTAGACACAAGAACCCCAATCCTTTGACCTGATAGTTGGAACTTTGATAACTTTAGTAGGGTGTTAAGTGATGGGAAATATCTCAATGCAATAGTTGTCAATACTTTAGTAACGGTACTTTCAGTGTTACTAACATTGTTTTTTACCATTTGCATGGGTTATAGTTTTTCACTACGGAAGTGAAAATACAAAAAACTGGTGTGGTTTTTCTTTCTTAGTGTGTTAATACTGCCTGAGTCTGCGCTTTTAATTGGTCAGTATCGGATTGTAATAGTTGCTAACTGAAATAACCCCAACAGTCCCTTGATTGTTCTGGGACTCATTATGCCCTTTGTTAGCAGTGTTTTTAGTGGGTTTATGTACCGTACTAGCTTTGAAGCCATTCCATCTCAATTAAAAGAGTCAGCACTCATTGATGGGTGTAATGGCTTTAACTACTTTTTGAAGATTGCTTTACCAATGGTGAAATCTACCAGTTGAACAGTGGGGATTTTAACTGCATTTAGTGCTTGAAATTCCTATTTATGACCATTACTGTTGTTGGGCAACAGGGTGGATTTAAACATTAACTTGTGGGTGTTACAACAAGGGATCTTGGATGCTAACAGTAGTGATGAACAGATCAGAACGCTGTTAAATCTCAAGATGAGTGCAGCGATTCTAGCCATCCTTCCGATGTTTATTATCTACTTTTTGTTCCATAAAAGGATTATGAATGCCATTAAAAACAGAGCCAACACCATTAAGGGTTAATATGCAAAAGTTTAAACAACTGGTTGGTGCAATGCACAGATGGGTCAAACTGGCACTATTAGTAATCATTGTGTTATTAGGGATTATCTTTTGTCTGTTTGCCATCTATGGCATTGCGCAAGTGATCATTACCATTAATGAAGGGGCACTTTTATAATCTTTGTTATGAAAAAAGGATCAATAACTGAAGCAATTAATGCCATTAAACAATTTGATAAGATCGTTATCTTTCACCATGTGCGCCCTGACGGGGATTGTTTAGGAGCACAACAAGGTTTGTTTCACCTCATTAAAGCTAACTTTAAAGATAAGGAGGTGAAGTGTGTTGGTAATAACAACAACCTGTTTAGCTTTATCAACATGACATTTACCAACCAAATTGATGAGAGCTTTTTAAAAGAAGCACTTGCGATTGTGGTCGATGCTAATTACAAAAACAGGATTGAATTGAGAGAACTGTTAGATAAAAACCTGTTTAAAGCAGTGTTAAGGATTGATCACCATCCCAATGAAGATGATCTAAACACTAGCTTTAACTTTGTTGAAGAAAGCTATGTAGCTTGTTGTGAGCAGATAGTGGAGATGGCCACAGTGGCGAAGTGGACCATACCACCAGTGGCTGCTACTTTACTATATATAGGTATCTATACGGATAGTAATAGATTTCTATATAGTAATACATCATATAGAACACTATACTTAGCAGCAATACTATATAAAGCTAAAGCTGATATAAGGATAGTACATGATCATTTAAACCATACTAGTTTAGCAGATCTTAAGTTTAAAAAGTATGTTTATAACCACTTTAAAACCCAAGGACAAGTGATCTATTTTATCTGTACTAAAAAGATCCAAAAGAGACTAAGAATGACTGCAGATCAATGTGCTAGAGTTAACTTGTTAAGTAACATAGCAGATTACAAGATCTGACTTTTCTTTATTGAACAAGCTAATAATGAGATCAGGATAGAACTGAGGAGTAATGGGATTAATGTCAGAGATATAGCCATTAAGTATGGTGGGGGAGGACATAATAATGCAAGTGGAGCGATCATTACTAACAAAAAACAAATTAGTGATGTTGTTAGTGATTGTGTGAAAAAAATTGTTTATAATTAAGTTTGTATGCACCAACCAAAGAAAAGACTGGCTAAGAAGTCTTGAGCCTTTCTAACCGCTGCACTTACCCTTGGGGTTATAACAGGTGTAGGTGGTTATTTTCTCTTTAACCAAAATAAGCAACGTAGTAGCGTGAGCAACTTTGCTTACCAACCCAAGCAGTTAAGTGTTAAACACCAACAAGCAGTTGATGAAACCTTAACCCCTTGGACTTGAAACAATAACAACTTCTCTTCACTAAAGATTACTGGAGAGAACCCAGGATCATTTGGATTAGTAAGAAGCCAAAATGACAACTTAAATATTTCAAGTGTTACAAAGAATCCTAATGATGATAATCTCGAGTATCTCAATGCTGTTGAGAAATACCTTGATGGTCAGCAAAACTTTGCAATCAGAAGGTATGATAACAACGGTAGAGCTTTATATGATATTAACTTAGCAAAAATGGAAAACCCCTCAACGGTGCAAAGGGGTTTAAATGGCGAGCCTATCTTTGATCCTTTTAAAGGCTTTGGTCTAACTGGTAATGCCCCTACTGATTGGAATGAGATCAAAGGTAAAGTTCCAGTAGAAGTAGTTCAATCCCCCCATTCCCCCAACCTCTATTTTGTGTTACTAGTGCCTAAGGTGGTAGTGGAGTACCACAAGCTCAGTAAGAAGGTTGTCAAAGAGAGTTTGGAAGTGGAAGCAACTGATTCCTTTGATCCCACCCAAAGGTTGAAAAAAGATAGTCCAGTGAAGGATTCAACAAAAACAGGGGAGAAACTCGAAACAACTGCTTCATCGATGAGTAGTGGTATGGCTACATCCACTCGCAAGGCCCTCAAAGTGGAGGTGGAAAGGGGGAGTAAAGTCAATCAAGGCGAACTACAATCCAACGACTTTGCTAAAAAGCCACTAAAGCATAAGAACAGTAGTGGGACAGATGTGAAGTTAGATTCACAGAAGGATTTTACTGAAGGAAAGGTTTGAAAACCGGTGTTGACTACGGATGAGATAGAAAAAAATAGGGGGATGGGGGCGACGGTGGTTAGTTTCTATGATGCACCCTACAGTGAAAACCATACTGCCTTTGGACTTGTTGATCACATCGATCCTAAAAAGATGGTTGAAAACTACCCACCAAGTTGAAAGACCCCGAAGTGAAACCACCATGGGATCTGGGATTACAACGCAAGAAACCTCTTGTTACAAACAACAGGGTTCTTTAACCCAAGAAGACACCCAGAGTGGTTTGATGAAGGACAAGCTAAGGCAGATAACACTAGCCCTGGCTTTAAGGTAGGGGAAAGTGATCACAAAAAAGACGGCTTTAAAGACTCTTCTTCTTCTTCTTCTTCTCCAATAGCTTTACCATTTGAAGCATACTTTGCTAATATTGGTAACATGGTTGCTATTGGCAACTCGGTATTTATCTTTGGTGGTAATGGTCATGCTACTAAGATGTTTACCACCAATCCCTTAAGTATTGGGGTATTTAGGATTAAATACACTGATAACTTTAGTAAGTCATCAGTAACTGGTTGACCATATGCAGTGTTATTTGGAGGATTAATTAATCCCCAAACCAATGGCTTGAAGGATCTTCCCCTTGGTACCAACAGGTGGTTTGAATATGTACCAAGAATGGCAGTTAGTGGGGTAAAATGGGTTGGTAATCAACTAGTGTTAGCAGGAACACTAACAATGGGTGATACAGCTACTGTACCTAGGTTAAAGTATGATCAACTAGAAAAACACTTAAACCTAGTTGCTCAAGGCCAGGGACTATTGAGAGAAGACTTGCAGATCTTCACTCCCTATGGGTGAGCTAATCGTCCTGATATTCCTGCAGGAGCATGACTCCAAGATGAAATGGGCAGTAAATTTGGTCCCCATTACTTTTTGAATAACCCTGATATCCAGGACAATGTTAATAATGATACGGTTGAAGCATTAATCAATACCTATACAAACACTGATAAGTTAAAACACGTTTATCCTTATAGATACAGTGGTTTGTATGCTTGACAGTTATTTAACTGGTCTAACAAACTAACCAATACTTCCCTTTCCGCTAACTTTGTTAATGAAAACAGTTATGCACCAAACAGTTTGTTTGCTGCTATCTTAAATGAAGATCTGTTAACAGGGCTAAGTGATAAGATTGGCTATGGTAAGGAGAATGAGTTTGCTGATAATGAAGCAGATAGGTTTAACCAACTTTTAAGTTTAAATCCTAGTGATAACACCAACTGAGCTAGGTATTTAAACGTAGTACAACGTTTTACTACCGGACCTAACCTTGATGGTTCTACCTTCGATCAGTTCTTAGACTTTCTCCCCTGAATCGGCAACAACAAACCCTTCTCCAACTCCCACACTGCTACCCTTTCTTCTTCTTCTTCCCCCCTCCCCACTTTTTCTAACATCAATGTCGGGGTTAAATCAATGATCACTCAACACTTAAACAAAGAAAACACCCGGTGGGTGTTTACCCCTAACTCTTCACCAGACATTTGAACTGGGGCTGGGTATCGCAAGGATGCATCAAACACCAGCGGCATCTCCTTGACAAGTGTGTTGCCTAGTAGTAGTAGTAGTAGTAGTAGTCCTTCGTTTAATCCCTCCTCTGCTGAAAATCAAGTCACCCCAGCTGGTGGCTCCTCCAAAAAACCAACCACCTACACCCATTTACCCAACAGTATCAGTCCCACCAGTGACTGGATCAACGCATTAACCTTCACCAATAAGAATAATCCGCAGCGCAATCAGTTGTTGTTAAGAGCACTGTTAGGAACGATCCCGGTCTTGATCAATAAGAGTGGAGGAAGTGGGAATGAGTTTAACCATACGAATGATCAGAAGTGGGATAAAACTGAGACAAATGAGGGCAACCTCCCGGGGTTTGGGGAGGTGAATGGGTTGTATAATGCCGCATTACTTTATACCTATGGTTTTTTTGGCACCAATACCAATAACTCAGATCCCAAAATAGGCTTTAAAGCTGATAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTAGTACACTAGTAGGTAGTGGGTTAAACTGAACTAGTCAGGATGTAGGTAATCTTGTTGTAATCAATGACACTAGCTTTGGGTTTCAACTTGGTGGTTGATTTATTACCTTCACTGACTTTATCAGACCAAGAACTGGTTATCTAGGGATTACCTTAAGTAGCTTACAAGATCAAAACATTATCTGAGCAGATCAACCTTGAACTAGTTTCAAAGGGAGTTATCTAGACAGTGATGGTACCCCTAAATCACTGTGAGATCCAACTGCTTTAAAACAACTACCAACTAGTTCAACTATCTCTGATACCAATCCTACCCTCCCCCCCCCCTCCTTCCTCTACTTTCAACCCAACAAGGTGAAAAGTGGTCAATACCAAACCACCAACACCTACAACAAGTTAATTGAACCTGACAAGTGACAATCAAGTAGTGATTTGAGTGGGATGAATAACTTGTTAAAACTACTGACAACTAAAAATGTGAAAGCAAAGTTGGGGAAGGGAGCAGCTTCTTCGCAGGGAAGTGGGGGGGGTGTTAGTCAAACGATTAATACCATCGCTACTACGGGGAATATTAGTGAAGGTCTAAAAGAAGAAACTAGTATTCAAGCAGAAACACTTAAAAAGTTCTTTGATAGTAAACAAAACAATAAGAGTGAAATAGGGATAGGTGATAGTACATTTACCAAGATGGATGGTAAACTAACTGGCGTAGTATCTACTCCTCTTGTTAACCTTATCAATGGTCAGGGAGCAACTAGTGATAGTGATACTGAAAAAATTAGCTTTAAACCTGGTAACCAGATTGACTTTAATAGGTTATTTACCTTACCAGTAACTGAACTATTTGATCCTAACACGATGCTTGTCCATGACCAGTATGTACCACTATTGGTTAACTTACCTAGTGGCTTTGATCAAGCTTCAATCCGCTTAAAGGTAATTAGTTACTCAGTAGAAAACCAAACCTTAGGAGTAAGATTAGAGTTCAAAGATCCTGATACTAACCAGTTTATTCCGGTACTAAATGCATCAAGTACTGGTCCCCAAACTGTCTTTCAACCCTTTAATCAGTGGGCAGACTATGTCTTACCTTTGATTGTAACTGTTCCTATAGTAGTGATTATCCTTAGTGTTACTTTGGGATTAACGATTGGAATTCCAATGCACAGAAACAAAAAGGCATTACAAGCAGGGTTTGATCTTTCTAACAAAAAGGTTGATGTTTTGACCAAAGCAGTTGGTAGTGTCTTTAAAGAGATCATTAACAGAACAGGGATCTCTAACGCTCCTAAGAAGTTAAAACAAGCTACACCAACCAAACCAACTCCTAAAACCCCACCAAAACCTCCAGTAAAACAATAAGATGAAAACAATAAGAAAACAGATTTATAAAAAAGCATACTGGTTACTATTACCCTTTCTACCATTAGCACTAGCCAATACCTTCCTTGTCAAAGAGGATAGTAAGAATGTTACTGCTTACACTCCCTTCGCCACCCCCATCACCGATTCTAAGGCTGATCTGGTTAGTTTGGCACAACTTGATTCTTCCTATCAAATCGCTGACCAAACCATCCATAACACCAACTTGTTTGTGTTGTTCAAGTCTAGGGATGTGAAGCTTACATACAGTTCAAGTGGCTCAAATAACCAGATTAGTTTTGATTCAACTAACAACAAACCATCCTACATCGTCGAGTTTACTAACTCCACTACGGTAGGCATCAAGTGAAGCGTGGTGAAAAAGTATCAGTTAGATGTGCCAAGTGTTTCAACAACCATGAACCAAGTTTTGCAAGAATTGATCCTAGAACAACCTTTGACTAAGTATACGCTTAATAGTAGTTTGGCCAAACAAAAGGGCAAAACGCAAAGGGAGGTGCATCTTGGTTCAAATATGGCAAATCAGTGACAATCGATGCGTAATCAACACAACTTAGACAACAATCCCTCTCCCAATGCATCAACTGGATTTAAACTCACTACCGGCAACGCATATAGAAAATTGGATCAATCCTGACCAATTTACCAACCAATTGATGGGACCAAGCAGGGCAAAGGGAAGGATAGTAGTGGGTGGAGTTCAACTGAAGCAACAATGGCAGCAGGGGATGCGCCCAGTGTTACTGCAGGGGGAAGTGCTTCTGGATCATTTAATAAATACCTCAACACCAAACAAGCGTTAGAAAGGATCGGCATCTTGTTTGATGGGGATGGAATGAGGAATGTTATCACCCAGCTCTATTATGCTTCTACCAGTAAGCTAGCAGTCACTAACAACCACATTGTCGTGATGGGTAACAGCTTTCTACCCAGCTTGTGGTACTGGGTGGTGGATCGGGGTGCAACAACTGATTCATCATCAAAACCCACCTGGTTTGCTAACACCAATTTAGATTGAGGGGAAAACAAACAAAAACAATTTGTAGAGAACCAGTTGGGGTATAAGGAAACTACCAGTACCAATTCCCACAACTTCCATTCCAAATCTTTCACCCAACCTGCATATCTGATCAGTGGCATTGACAGTGTCAATGATCAGTTGATCTTCAGTGGTTTTAAAGCAGGGAGTGTGGGGTATGATAGTAGTAGTAGTAGTACTCAAACCAAAGACCAAGCACTTGCTTGATCAACAACAACTAGCTTAGATAGTAAAACGGGGTATAGGGATCTAGTGACCAACGACACGGGTTTAAATGGTCCAATCAATGGGAGTTTTTCAATCCAAGACACCTTCAGCTTTGTTGTTCCTTATTCGGGGAATCATAACAATAATGGAAATATCAGTGGCAATGGAACCATTCAAACGGCTTATCCGGTGAAAAAAGATCAAAAATCAACAGTAATGATCAATTCTTTGATCAACGCTACGCCGTTGAATAGTTATGGGGATGAGGGGGTTGGGGTGTTTGATGCGTTAGGTTTAAACTATAACTTTAAATCTAACCAAGAACGTTTACCTTCCAGAACTGATCAGATCTTTGTTTATGGGATTGTCTCCCCTAATGAATTGCGAAGTGCTAAAAGTTCTGCTGATTCAACTGGTAGTGATACAAAGGTAAACTGATCAAACACCCAATCACGTTACCTCCCTGTTCCCTATAACTATTCAGAAGGGATCATTGATGCAGATGGATTTAAGCGTCCTGAAAACAGGGGTGCTAGTGTAACTACCTTCTCAGGGATTAAATCAATTGCCCCTGATGGTTTTGCTAACTCAATAGCTAACTTCTCAGTTGGGTTAAAAGCAGGAATTGATCCTAACCCAGTGATGAGTGGTAAGAAAGCTAACTATGGAGCGGTTGTGTTAACACGGGGGGGTGTTGTTAGATTAAACTTTAACCCTGGTAATGATTCATTGCTTTCAACAACTGATAACAATATAGCACCTATCTCCTTCTCATTTACTCCGTTCACAGCTGCTGAGAGTGCAGTGGATCTCACTACCTTCAAAGAAGTTACCTATAACCAAGAATCAGGATTGTGGAGTTATGTCTTTGACAACTCCTTAAAACCAACCCATGATGGTAAACAAATTGCTGTCACTGATAACAAGGGCTTTAGTGTTATCACTGTCTCAAGAACTGGCATTGAACTAAACCAAGACCAAGCTACTACAACTCTTGATGTAGCACCTAGTGCACTAGCAGTGCAATCAGGGATCCAATCTACCACCCAAACCCTAACTGGAGTACTCCCACTTAGTGAGGAATTCAGTGCAGTTATTGCTAAAGATAGTGGTCAAAATAAGATTGATATCTATAAAAACAACAACGGGTTGTTTGAAATTGATACCCAACTAAGTAATAGTGTTGCCACCAACAACGGTGGGTTAGCACCTAGTTACACAGAAAACACGGTTGATGCATGGGGTAAAGTTGAGTTTGCTGATAACAGTGTATTGCAAGCAAGAAACCTAGTTGATAAAACTGTTGATGAGATCATCAATACCCCTGAAATCTTAAATTCCTTCTTTAGATTCACCCCTGCTTTTGAAGATCAAAAAGCTACCCTTGTTGCTACTAAGCAAAGTGATACGTCACTTAGTGTCTCACCAAGAATCCAGTTCTTAGATGGTAATTTCTATGATCTTAACTCCACCATCGCTGGGGTACCTTTAAACATTGGTTTCCCTTCAAGAGTGTTTGCTGGGTTTGCAGCACTCCCTGCATGGGTGATCCCTGTATCAGTAGGTTCTTCAGTTGGGATCTTGTTTATCTTGTTAGTCTTAGGACTTGGGATTGGGATCCCAATGTACAGGGTAAGAAAACTCCAAGATGCATCGTTTGTTAATGTCTTTAAAAAGGTTGATACACTCACAACTGCTGTCGGTAGTGTGTACAAAAAGATTATTACCCAAACTGGTGTGGTGAAAAAAGCACCTAGTGCATTGAAAGCTGCTAATCCTAGTGTTAAAAAACCAGCAGCCAAACCACCTGTTCAACCACCAAGTAAACCTGAAGGGGAACAAAAAGCTGTTGAAGTTAAGTCAGAGGAAACCAAAAGTTAGTTTTTAACCTTTCAATAACCTAAAACACAATCTTTAAAACAAGGTTGTGTTTTTTTATTTTTTGTCACTTTTCACTCAACTTGCAATTTAGAGAGTAGATATGAAAAGGACAGTTAAAAAAATAAAACCTGACCACTTGTTCAATAAAAAGCAGTGGCACTTACTGAGTGAAGAGATCAGTGATAACCCAATGATTAAGCGCTATTGACTCAAACAACAAAAGCGTCAACTTGCCAAACTAAAAAAACGCCGTTATTTTAAAAATTAACTATGTATTCTACTTTAAGACAATATAAACCATTGAAAAGAAAGAAATGGTTAAACAAGATCTCAAAACGGAAGTTACAACAACTTTACAACAAAGCTTATTAGCTTTGTTTTTTATTTTTACTAGTGCCTAAGGTGGTAGTGGAGTACCACCAACTTGATAAGAAGGTTGTCAAAGAGAGTTTGGGGGTGGATACATCAGGTTCAACTTTGATCCCACCCAAAGGTTGAAGAAAGATAGTCCGGTGAAGGATTCAACAAAAACAGGGGAGAAACTCGAAACAACTGCTTCATCCATGAGTAGTGGTGGGGCTACCTCTCCTCGCAAGGCCCTCAAGGTTGAGGTGGAGAAAGGGGAGTAATGTCAATCAAGGCGAACTACAATCCAACGATTTTGCTAAAAAGCCACTGAAACATAAAGAAAATAGTGGGGAAGTGAAGTTAGAGGCAGAGAAGGATTTTCCTGAAGGAAAGGTTTGAAAGCCGGTGTTGACTACGGACCAACTTTCAAAAAATAGGGGGATGGGGGCGACTTAGACTTTCTCCCCTGAATCGGCAACAACAAACCCTTCTCCAACTCCCACACTGCTACCCTTTCTGTTAGTTCAAATACCCCACTCCCCACTTTTTCTAACATCAATGTTGGGGTTAAATCAATGATCACTCAACACTTAAACAAAGAAAACACCCGGTGGGTGTTTACCCCTAACTCTTCACCAGACATTTGAACTGGGGCTGGGTATCGCAAGGTTAAGGTAACAAAAACGGCATCCCTTTTGATAATGTGAAGCCTAGTAGTAGTAGTACCCTCTTTGATCCCTCCTCCATGGAAAACAAAGTCACTTCAGGTGGCTCCTCCAAACCAACCACCTACACCCATTTACCCAACAGTATCAGTCCCACCAGTGACTGAAGCAATGCGTTGACTTTCACTAATAAGAACAACCCGCAGAGAAATCAACTGTTGTTAAGAGCGTTATTAGGAACTATCCCGGTCTTGATCAATAAGAGTGGGGAGGGTGGGGAAGAGTTTACCAAGGATAGTGAGCAGAAGTGGGATAAGACAGAAACCAAAGATGGCAACCTCCCGGGGTTTGGGGAGGTGAATGGTGGTTTTTATCAACTAAATAAAAACTTATTAGCTTATTTTTATTAGGTTTTTACTTATTTAATAGTTAAAAAAGTTTTGAATTTTTCTTAGTTTTTTATTTGTTTAATAGTTAAAAAACACTAGGCTTTACCTTTATTTAATTAATAAAACCTTTACCCCTATTACCAAACCATCCATAACACCAACCTGTTTGTGTTGTTCAAGTCCAAGGATGTGAAGCTTACATATAGTTCAAGTGGCAGTAACAACCAGATTAGTTTTGATTCAACTAACAACAAACCATCCTACATCGTCGAGTTTACTAACTCCACTACGGTAGGCATCAAGTGAAGCGTGGTGAAAAAGTATCAGTTAGATGTGCCAAGTGTTTCTAGCAACATGAACCAAGTTTTGCAAGAATTGATCCTTGAACAACCTTTGACTAAATATACCTTAAACAGTAGTTTGGCCAAACAAAAGGGCAAAACGCAAAGGGAGGTGCATCTTGGTTCAAATATGGCAAATCAGTGACAATCGATGCGTAATCAACACAACTTAGACAACAATCCCAGTCCCAATGCATCAACCGGATTTAAACTCGACAAAGGCAATGCATATAGAAAACTAAGTGAGTCCTGACCAATTTATGAACCGATTGATGGGACCAAGAAGGGCAAAGGGAAGGATAGTAGTGGGTGGAAGTTCAACTGAAGAAAACGAAAGCTAAAAAGTGATGCGCCTCAAGTACAGGTGGGGGTGCTTCTTCTGGAACATTTAAAAAATACCTCAACACCAAACAAGCGTTAGAAAGGATCGGTATTTTGTTTGATGGGGATGGAACGAGGAATGTTGTTAGCCTCCTTCCTCTACTTTCAACCCAACAAGGTGAAAAGTGGTCAATATCAACAAAATAACACCTACAACAGGTTAATTGAACCGGAAAGTGCAACAAGTGCAGCAACTAACATGACCAACTTGTTAAAACTACTGACAACTAAAAACATCAAAGAAAAGTTGGGGAAGGGAGCAGCTTCTTCGCAGGGAAGCAAGACACCTTTTCCTTCGTTGTTCCTTATTCAGATAGTCATAGTAATCAAACTTCATCAGGAACCATTAAAACGGCTTATCCTGTGAAAAGTGATCAAAAATCATCAGTAGCGATCAATTCCTTGATCAACGCTACGCCGTTGAATAGTTATGGGACAATAAAAAAACTACCTGTCAACAAAAAACAGGTAGTTAATTATTTATCCAATTAGTAAGTTGCCTATGGGTTAACAAAGAGATGCACTTAATATACGACCAATCTAATTTATTTGAAAGCGTATCTTTTATTAATGAATGTGGAACTAAGCAACTATTATATTAAACACAGCAAAAGCGTGTAATTTTTTATTAAAAGCGATTTAGTGGGAACAGAAATAAAAGATTAACATGACTTTATACAACTTCTGTTCCCATCTACTAAATTGCACAAAAAAAGCTTTTTGACAAAAAAATAGTTATTTTTTGTAATTGCTTAATTTAATAACAAACTAAACTATTATTGTTACTTAATGATTTTTTGATCTATATAGCACAATTAATATAACTTCATGATTGATCAAAACAAGTTAATTACTAAGTGAAAAAAAGCATTTGCAAAAGCTAAGAATTTAACTACTCTAGTTAATCTTAAGAACACTTTACACAACAGTGATTTAAAGCCATTACTCCAAAAGATTAAAACCGCTACAAAACTAAGTGAAAAAAGTAGTTTAGGTAAGCTTTATCAATCACTTGATATTCAACTAACTGATCTGTTAACTAGTTACAAAAAAACCTTTGAAATAAATAACCAAGTTAGTCAAAAACCTTCACTTGATGTGATGCTACCAGCAACAGAGTTTACCAATGGTTCTAATAACGCACTATATCAGGTTATTGATAATTTAGTTGAATACTTTAAAAGCTTTTTATTCACAATTAATTTTGATAGTGAACTGACCAGTATTAGTGACTGTTTTGATCTTTTAAATATCCCTAAAGATCATTCCAGTAGGAATGAATCTGATTCTTTTTATATCGATAAAACCAGTTTATTGAGAACCCATTGTACTGCTACCACGCTAAAAGCAGTCAGAACTTCTAAAAAAACTAATAATCCTGATATCAGGGTTGTCTCTTTAGGAGCGGTTTTTCGTAATGATAGTGATGATGCCACCCACTCCCATCAGTTTACCCAACTTGATTTTATGTGGATTAAAAAAGGGCTTTCATTAGCTAATTTAAAGTGGTTTATTAACAATATGATCACCCATTTCTTTGGGGAAAATACTTTTACTAGGTTTAGACTATCCCACTTCCCATTCACTGAACCCTCGTTTGAAATTGACATTAGGTGTTGGTTATGTCAAAATGGTTGTTCTATTTGTAAGCAAACCAAGTGAATTGAGATCTTAGGGGCGGGGATCATCCATCCCCAGGTGATGAATAACATGGGAATTGGGGATACTGAAAATATTACTGGGATAGCAGCAGGAATTGGGATTGAACGCTTAGCAATGTTAAAGTATGGGATTGATGATATCCGTGATTTTTATGATAACAACTTTAAGTTTTTAACCCAGTTTACTGACTAAAATATGTTGATATCAAAAAAAACACTTGGCGTTTTAATCCCTGATATCTTTAGTTTTTCTAATGATCAAATTGCCCAAAAGTTAGAACAAATGGGGATTGAAGTGGAATCAATTAAGCAGTTTAACAGCCCTGATTACCTCCAACTTGCAAAGGTTGTATCAATCCAACCCCATCCCCATGACAACAAGCTTTTTATCTGTGAATTACAAATTGATAAAAACAAGTTTATTAATGTTGTTTCCAATGCTAATAACATTAACAATCCTGATAATATCAACAAGTTTGTCATTGTTGCAAAAAAAGGAACTGAGTTACTCAACGGGTTAATTGTTAAAACCCAAAATATTAAAGGGATCATTTCAGAAGGGATTTTATGTAGCTATATTGACATTAACCCCTTCAGTAGACAGATCATTGAAAAAACAGAAGTTGCTGATGCGATTATCATTGATCATGTTAGCAATGATCATGACTGAAACCAATACCTCTCGTTTTTAAGTTTGGATGATGTGATCTTTGATGTTAAAACCCCAACTAACAGAGCAGATCTTCATAGCTTAATCTTTTTAGCAAAAGAACTTGGGGTACTTTTGAAAACCAAAACCTTTTTAAAACAAAAAAGTAGTGTTGTTAACCATGACTTTTTTAAGTTTCCCCTAAATTTAAAAAACAAGTTAAAAGCGAATTATTTTGGCGGTTTGTTCTTAAGACAAATTAACCAACATAGTTCACCTTGAACAGTTAAAGGACTGTTAATTAACCAAATGATCAAACCAGTTAACTATTATGTTGATAAAGCTAACTTAGTAACAGTGTTCACCGCTCAGCCAATCCATTGTCATGATGCAGATAGAATTGTTGGTAACATTGAACTTAAACAAGCAACCCATAATGAAACTTTTGTTGGACTTGATGACAAGCAATATGAGATTGAACCAGGGGATATTGTTGTTTGTGATGAGAAGGGCATTATTGCACTGGTAGGGATCATTGGTTCAAAGCGCACAATGGTCCAACCTACAACAACTAACATCTTTTTTGAAGTTGTTAACTGTAACAGTGAAACCATTAAACAAACTGCCAAGCGCTTTTTGATCAATAACTTTGCCAGTAAGTTTATGGTTAAACAGATTAGCTTATTAGCTACTGATAACTGTTTAAACTACTTACAAAACAGTTTACTAACCACTGATAACATTGGCAAAATTAGCCACTTTTCAAGTTCGCTTAAAGTTGAACCATTTAGTAAAAAGCTCACAGTGAATTTCCATAAGATACGCCAACTAATTGGCATTGAAAAAAAGGAACTAACTGATCAAACCATTAAAAAAAGCCTCAGTCAACTAGGGTTTAAAGTTGACAACCAACTTCTCAAAATCCCCAGTTACAGACAAGACATTAATACTTGACAAGACATTAGTGAAGAGATTGTGAAGTTAATTGATATCAATAAGTTAAAACCAATTGGGATCACTAGTAGTTTTAACTTTGAAAAGTCTAGTTACTTTAACACTTTTAATGCTTTAACAAAACTAAGAAAAAAACTGCAAACACTTGGTTTTCACAACGTTATTACCTACCAGTTAACTGATCAAAAAAGTGCAAAAACTTTTAATTTGTTTAACTTAGAAAATTTCATCACCATTAAAAACCCAGTGTCCCAAAACCATTCTGTAATGCGTGTTAGCTTAATTGATTCACTGTTAAAAGTGCTACAAACCAATAACAACTATAAGAATGAACTGGTGAACATCTTTGAGTTTTCCTTTATTAAAACCCAAAACAATAGTGAACTGCACCTGGCAGTATTATGAGTTGAAAAACTGTTTACTTCTAGTTTCAATCCTATGCAAAGGATAAGCAATGATGTTTTTACTATGAAGGGATTAGCAAAACTCATTGTTGCTAACTTAGGGTTTAGTTGTGACTTTGAACCACTTGATGATAGTGACTATTTTGTTAATAATCAAAGTTTAAAAATAGTAGTTTTTAACGAACAGATCGGTTTTATTGGGCTAATTAAAGAATCATTGTTAAATAACTATGATCTGAACAATAAACCCATTTATTGTCTTGAAATCAACTTAGATAGGATGCTCTCTTCTCTAAACAGGATTGAAAAAAACTACCTTGGTTACAGTAAACTACAACCTGTTTGCAAGGATCTTACCTTTAGTTTTACCAACCCTGCTAGTCACTTTGATCAGTTTGCTAACATGATCAAAAGGATAACTGGCATTGAAAGTTGAAAGTTAATTAGTGTCTTTGAAACTATGCAAAACAACCAACTGATCACTAAGTACACCGTTCGTTATTTTCTGAAAAATGATGCTAACAAACCACTAACTAACCAAACAATTGAACTTATCACTAATAACTTAAAACTCCAGTGTGAAAAACTAAAAATTAAATTAGATATTTAGAATTACTTATTACTATCAAGATAGTTACCAACTATTTTGTTAGTAAAACTTAATACAATTGGCACAAAACGCAAAACACCCTTCTAAAAAAGAACAAAAACCACTCGTTAATGAACAGATTGCTTTCAATCAGTTCACTTTAATTGATGAAAACAGTACTAATTTAGGGATAGTTAAGATGGAAAACGCTTTAAAGTTAGCACAAGAAAAACAGTTAGATCTAGTTCTAATTGCTCCAAACCCAACCAAACCGATCGTTAAGTTGTTGGACTTTGGCAGATATACCTATGATTTAAAGCGTAAGAAAAGACAAGCCAAGAAAAACCAAACAATCATCCAAACCAAAGAAGTTGTTGTCAAACCAACGATTGCTAAACATGATTTAGAATTTAGAGCAAAACAGAGTAAGAATTGGATAGAAAAAGGTCATCATGTCAAGTTTATAGTCCGTGCCTTTGGCAGGATTAGCACCAGGATAGAGTTAATTGAAAAGGTGTTTGATGACTTTTACCAGTTAGTTAAAGATGTAGTTGAGATCCAAAAACCTTTAACCGCTTCTTCCAAAACGATGTACGCTGCTCTATTAGTACCTTTAAAAAGATAGTTATGAAAACCAAAAGTGCTGCAGTAAAACGCTTTAAACTCACCAAATCAGGACAAATTAAGCGCAAACACGCTTATACTTCCCACCTCGCGCCCCACAAATCAACCAAACAAAAGCGCCATTTGCGCAAGCAAGCTACTGTGAGCAACAGTGAATTGAAAAGAATTGGTATTTTAATTTAGTTATGCGTGTTAAGGGAACAAATACAACCAGGATTAGAAGAAAAAAATGGTTAAAACAAGCTAGTGGTAGCTTTGGGACAAGAAAAGCTTCTTTTAAGGCAGCTAAACAAACTGTTATCCAAGCAAGCAAGTATGCTTACCGTGATAGGAGACAGAAAAAACGTGAGTTTCGTTCGTTGTGGATCTTAAGGTTAAATGCTGCACTGCGTGCACAAGGGATGACTTATTCAGTGTTTATCAATGAATTGAAAAAAGCCAAGATAGTCATTAACAGAAAGGTACTTTCTGAACTAGCAATTAAAGAACCTAATAAGTTAAATCTGATTATCAATACCATCAAAAAACCAACTAATAAACCAACTGTTGCAAAAACTTAGTAACGTTTTTAAAGTTAAGTTTTGCCACCTGTTTAAACTGTTCAATTTGACTAAAACCGCGTTGTTGGTTAAGAAATAACAACGCTTGTTCAATGCCATGACTACTGCCTTTGGGTAAATCATAGTTTACCTTTTGGTTGAGTAATTGCAGCTGATTAATAAAGCTATCTCTTGCTAAGATAGATGCAGCTGCAATCACCAGTGACTTAGTTTCACCATTAATTAGAAACTGATCAGGTAATAACACTGTTTTATCAGTGAAATTAGTTAGCTTATTTAGATAGTTAACAAATAACTCTTGATTGGCAAACTGGTCAATGCTAATTGTTACTGTTTGTCTTAATAACTGATTTTTTTCCAGTAACTTTTGGTAAAGCTGACAGTGTAAATTGGTCAAAAGTAAATTGGTATTTTTCAGTGATTTTGTCAAATCATTATACTGCTTGGGATCTAATGTAATTGTATGGTGATCCATTACTGTTGTTTGGATCGATTTAGCTAACAGTTTGACAGTGTGATCACTGAGTTTTTTTGAATCAGTTACTTGTAAATTTTCAAGGTTAATAAGCTGTTTTTTTTCAATTAAAACAGCACTAACACAAATCCCACCAAAACTATCACCCTTGCCAGATTCATCACTGCCAATTAAATAAAAATCAGCTGGTTTGTAATGTTGCAACTATCTAGTTAATTAATAAAATTATACTTACTCTGATTGATTCTTAATGGCTGAACAGAAACGTGATTATTATGAAGTGTTAGGAATAACCCCTGATGCTGATCAATCAGAGATTAAAAAAGCCTTTCGTAAGCTGGCTAAAAAGTACCATCCTGATCGTAACAACGCGCCTGATGCTGCTAAGATTTTTGCTGAAATTAATGAAGCAAATGATGTTTTATCAAACCCCAAAAAAAGAGCTAACTATGATAAGTATGGTTTTGATGGGGTTGATGGTGAACCTGCTTTTAACTTCCAAGCAGATGTTTTTCAATCCTTTTTTGAAGAGATAGCAAAATCAGGGGTTTTTAACAACCAAACCAATCCTGAACAAAAAGAAAAAAGAAACGTTACCACTGGTTCTCCAAAAAACCTAAGCAAGAACAACCTGAAATTAACTTAGATCACGTTGTTGAGCAAACCATTAAAAAGGTGCAACAAAACCAAAACCAAAACAAAGACCCAGATGAATTACGTTCTAAGGTCCCTGGAGAGGTTACTGCTAGTGATTGGGAAGCATTGGTTGGTGATACTAGGTATGGGTATTTTGATGAAACAGGGGATTGGAGTTGAAAGGGTTACTTTGATGAACAGGGCAAATGGGTTTGAAACGAACCAGTTGATTCTGAAACCAGTGAGGTATCAGTTGAACCTGAACCAACCCCAGTTGCCCCTGAAGCTAGTTTTGAAGAAGCTCAACCTGAAATTAATGCTGAACCAGAAGCTAGTTTTGAATCAACTCCAACTCCAGAACCAGTTGCCCCAGAAGCTAGTTTTGAAGAAGCTCAACCTGAACCAACTCCAATTCCTGAACCAATCCCAACCCCAGTTCAAGTTCAACCCCTGTTGTTAGATCTCAACCTCTTTACTATCCCAACTAAAGCTACTAAGGATGATCTTTTGTTTGACAACATTAACCTCACTACCTATGAACAAGTTGTTGATTATCTCAACAGTCAAGCAACCCCTAATTTAGCTAAAACCGATGGTGAATTGCAAACGATTGATGGTACCAACCCATTGTTATTAGAACAGTGCAAAAAGATCAAAAAACAAGCAGAACAACTCTTTAAAAAACTCTTTTTAAAAAAACAACTCCCCTTCATCACCCAACCTGAAGTTGTTGAGGAAAGTAAAACCAGTTTTGATGAGAACAACGTTAACCTTGTTTACTTTGAAAAGGTCCCTGAAATCCTTTTCATTAACCAACAACCTAAGGAGGTAAAATACACCCGTCAAGTCTTTGATGGGTTGACAAACAAAACAACTAGTGAAACGATTACACTAGAGATCCAACTACTCCAAACCCCAAAAGAGACTGTTAGTGCCATTTTTAAAGGCTTTGGTAATGACCATGGCAAGGGCTGTGGGGATTTAAAGATTGTTTTTGAAAAGATTAAAAGCCCCTTTTTTCAAGTCAATGAGGATGGCTTGCACTCTGCTTGCATCATTGACCCTTTAGTTGCTTACAACGGCGGGATTATCGATGTGTTTGGGCCCTACACTAACTTCCAAGTTAAGGTAGATGGGGAGATAGACATCAATGCCATTATGAAGTTTGAAAAACTAGGCATTGCTAAAACCAAACGCAAGGGCGATCTTTTTGTCCATCTCTATTACAGTAGTGTCCCTAAAAAGAAACTCACCACTAACCCCCAAGTTCAACAGTTCTTAGAACTTTTACAAGCTGAATATGAACTGTTGCAAGACAACATCAAGAGCTTAAAGTACTTTAAAAATAACCTAGTTATCCCCAAAAAGCCACTTGATCAACAAAGCTATCAATACCTCTCCCAAGAACCCATTAGTTAGAATTTGTTAATATGTGTGAAAAATCACAAACAATTAAAGAGCTTTTAAACGCCATTAGAACCTTAGTTGTCAAGAACAATAAAGCTAAGGTTAGTATGATTGAAAAGGAACTGTTAGCTTTTGTTAGTGAACTTGACAAAAAGTTCAAACAACAACTCAACAACTTCAATGAACTACAACAAAAGATCCCACTACTCCAAAAAGCTAACGAAGAGTTTGCTTTAAAGTTTGAAAGGATGCAACGCGAAGCACAAAACCAGATCCAAGCCAAACTAGATGAGTTGAATCTTAAAAATAAAAAGGAGTTAGAACAAGCCAAGAAATATGCGATTGCCAAAACCCTTGACCAACCCTTAAACATCATCGATCAGTTTGAAATCGCGCTTTCATATGCCCAAAAAGACCCTCAAGTAAAAAACTATACCACTGGTTTTACCATGGTACTTGATGCTTTTTCAAGGTGATTGGAAGCAAATGGGGTTACCAAGATTAAGATTGAACCAGGGATGGAATTTGATGAAAAGATTATGTCTGCATTGGAACTAGTTGATTCTAACCTTGCTAAAAACAAGGTAGTAAGAGTCTCAAAATCTGGCTATAAACTCTATGACAAAGTGATCCGCTTTGCATCAGTATTTGTCAGCAAAGGTAATAAAAAATCATAATAACAACTTATGAGTGAACAAAAAAGAAGAACAATCCAAATTGCGATAAGTGAAGACCACTATGAAGAGTTACAAAAGGCATTGGAACTACTTAAAGGGACCCAATTACCCTTTTCAACCACTGTTGAACAGTTTGTGGAGTTAATCTTATCTAACTATGTAGCTACTTCCAATAAGATTAGTAGTTTAGCTAAGAGTGGTTTTGATGTAGCTTCATTGCAGCAAGAACTTGAGAAGATAGGTAACCTTAGTGGGGTTGATGATAACCTCAAGGGTTTTCTCTCAGAACTGTTGAAAACCTCAAGGAATGGGTTTAGTAACCCCAATAAAGATGGCAAAAAAAATGATGACGATAATAACTCGTCATCAAAATCATAGTTATATAAAACTTTAAGTGGTGCCGAATAACAGAGTCGAACTGTTCTCTGATCCTTACCATGGATCTGTTTTGCCCCTAAACCAATTCGGCAGCAAAGCTAATTTCAATTTTAAATTCAAACTTTAGATGAAAAGTAACTACAGTGCAACTAACATCAAGATCTTAAAGGGTTTGGATGCAGTTAAAAAGCGTCCGGGGATGTACATTGGTTCTACTGATAGTAAGGGTCTGCACCACATGCTATGGGAAATTCTTGCTAACAGTGTTGATGAAGTTTTAGCTGGTTATGCAACCAATATTACTGTTACTTTAGATCTCAACAACACCATTACTGTTAGTGATGATGGCAGGGGTATTCCCTATGAGATCCACCAAGACAGTAACATCTCTACGATCGATACAGTTTTCACCTTTCTCCATGCAGGGGGGAAGTTTGATGATCAGTCATACAAACTAGCAGGGGGATTACATGGAGTTGGTGCATCAGTGGTCAATGCCTTAAGTGATCATTTAGAAGTAACAGTGAAAAGAAATGGTCAGATCTACCAATCAGTTTATCAAGCTGGGGGTAAGATCATCCAAAAAGCCAAAAAGATTGGTGATACAACTAGCCATGGTACCACTGTTAGTTTCCATGCTGACCCTAAGGTCTTTAAAAAGGCTCAATTTGATAGCAACATTATTAAAAGCAGGTTAAAAGAGCTAAGCTTTCTGTTTGCTAAACTAAAGCTCACTTTTACTGATCAAAAAACTAATAAAACCACTGTTTTTTTTAGTACCTCAGGACTAGTTCAGTTCCTTGATGAAATTAATAATACTGTAGAAACACTTGGCCAAAAAACACTGATTAAAGGTGAGAAGGATGGGATTGAAGTGGAAGTGGTTTTCCAGTTTAACCAATCAGATCAAGAGACAATCTTATCATTTGCTAACTCGATTAAAACCTTTGAAGGAGGGAGTCATGAAAATGGGTTTTGTCTTGCCATTAGTGATGTGATCAACAGCTATTGCAGAAAGTACAACTTACTAAAAGAAAAAGATAAAAACTTTCAACTTAGTGAGATCAGACAAGGGTTGAATGCTATTATCAAAGTTAACTTACCTGAAAAAAACATCGCTTTTGAAGGACAAACTAAGAGTAAGTTGTTTTCAAAGGAAGTGAAAAACGTTGTTTATGAATTGGTCCAACAACACTATTTCCAGTTTCTGGAAAGAAACAACAATGATGCTAAATTGATCATTGATAAACTACTCAATGCTAGAAAGATTAAAGAGCAAATCAAACAACAACGTGAGTTGAAAAAAAGTTTATCAAGTCCCCAAAAAGAGAAGATCTTATTTGGGAAGTTAGCACCTTGTCAAACCAAAAAAACCAGTGAAAAAGAGTTGTTTATTGTTGAAGGTGATAGTGCTGGTGGCACTGCTAAAATGGGCCGTGATAGAATTTTTCAAGCTATCTTACCTTTGCGCGGCAAGGTGTTAAATGTTGAAAAAATTAACAATAAGAAGGAAGCGATCACTAACGAAGAGATCCTCACTTTAATCTTTTGTATTGGTACAGGGATTTTAACTAACTTCAACATCAAGGACTTAAAGTACGGAAAGATCATCATTATGACTGATGCAGATAATGATGGCGCACACATCCAAATCCTCTTACTTACCTTCTTTTATAGGTACATGCAACCCCTAATTGAACTGGGCCATGTCTATCTAGCTCTTCCTCCTTTATATAAGCTAGAAACCAAAGATAGAAAAACAGTTAAATACCTCTGGAGTGATTTGGAGTTGGAATCAGTCAAACTAAAGCTTAATAACTTCACTTTACAACGATACAAAGGACTTGGGGAGATGAATGCTGATCAGTTGTGAGATACTACTATGAATCCAACTACCAGAAAGCTAGTGCAAGTAAAGCTTGATGATCTAATTAACGCTGAAAAGCAAATCAACATCTTTATGGGTGAAAAGAGTGATTTGCGCAAACACTGGATTGAAGCCAACATTAACTTTAGTGTGGAAAACTAATGGATCAAAAAAACAACAACCTCTTTCAAAAGGCAATTGAAGAAGTCTTTGCAGTTAGCTTTAGTAAGTATGCTAAATACATCATCCAAGATAGAGCTTTACCTGATCTAAGAGATGGGTTAAAACCAGTACAAAGACGGATCTTATATGGGATGTTTCAAATGGGCTTAAAACCCACCACTCCCTATAAAAAATCAGCCCGTGCTGTTGGGGAGATCATGGGGAAATACCACCCCCATGGTGATAGTTCCATTTATGATGCAATTATCAGAATGTCCCAAAGCTGAAAGAACAACTGAACAACTGTTTCTATCCATGGTAACAATGGTTCAGTGGATGGGGATAATGCTGCAGCAATGCGTTACACAGAAACCCGCTTAAGCTTGTATGGATTTGAACTATTAAAAGACATTGATAAAAAGTTAGTTAGTTTTATCAATAACTTTGATGATAGTGAAAAAGAACCAACGGTTTTACCAACCTTACTGCCTAACCTCTTTATCAATGGTGCGAGTGGGATAGCTGCTGGATATGCAACTAATATTGCTCCCCATAACACTAATGAACTATTAGATAGTCTTTGCTTGCGAATAGACCAACCTGATTGTGAACTTAAACAAATTTTAAAAATTGTTAAAGGTCCTGATTTTCCAACAGGGGGTAATGTTTATTTTGAAAAGAGTTTAAGTGATATTTATCAAGCAGGCAAAGGTAAATTTATTATCCAAGCTAAGTATGAAGTTAACAAGAACTTAAACCAGATTGAAATTACCCAAATCCCTTATGAAACACTGAAAGCTAACATTGTCAAACAAATTGAAGAGATTATCTTTGACAATAAACTATCTGCTATTGAAAGTGTCATTGATAGTTCAGATCGCAACGGCATTAGGATCATTATTAAACACAAGGACTTTTTGCCTGCTGAGAAGATCATGGCCTTTTTGTTTAAACACACCCAACTCCAAGTGAACTTTAACCTTAATAACACCGTGATTGCTAACCGCTTTCCCATCCAAATTGGTTTACTAAGTTACCTCGATCATTTTTTAAAGTTTTGTCATGAACTAATTATTAATAAAGCTAAGTATGAACTTGAGCTTGCAAGCAAGCGCTTGGAAATTATTTTAGGACTAATTAAAGCGATTAGTATCATTGATAAAATCATCAAATTAATTAGATCAGCAGTTGACAAAAGTGATGCAAGAGAAAAGTTAATTGATAACTTTAAATTTACTTTTAACCAAGCAGAGGCAATTGTTAGTTTGCGACTTTACCAACTAACTAACACTGATATTTTTGAACTTAACCAAGAACAAAATGAACTTGAAAAAACTGTGATTAGTTCAAAGCAACTAATTGCTAGTGAAAAAGCAAGAAACAAACTCCTAAAAAAACAGTTTGAAGGTTATAAAAAGCAGTTTCACCAGCAACGAAGGTCACAAATATGTGGCTTTATTAACCAAAAAAAGGTGGAGGAAAGTGAGCTAATTGAAAACAAAACTTATGGGGTTTTAATCACTAAAGCTGGTAACTACCATAAGTTTGAATCTAACCAACTATTAAAAAGCACCACTGATTTTAAAAGTGAGAGTGACACAATTATCTTTGCACAAACTATTGCTAATACCGACCAAATTTTTATTGTCACTTCACTAGGTAACATTATTAATATCCCTGTTTATAAATTAGCTTTCAATTCCAAAAATAAACTAGCAAGTTTAGTTAGTAAAAAACCAATCCTTTTGGAGTATGAAACGATTGTTTTTGTTGGAACAATGAACAGTGTAAACCAACCAATCCTTGTTTTAACTTCCAAACTAGGAATGGTTAAACGGATTGATTTAACCAAACTTAACAATAAGCCACTTAAAGCTACTTTGTGTATCTCACTCCGTGATAAAGACCAGTTAGTAAGTGCATTTTTACAACAAGATGATAAACTGATCTGTTTAGTGTCTGATCACAACTATTACACAGTTTTTCACACCAATGAGATCCCACTAATTAGTAGTAAGGGGATGGGAGTGAAGGGGATGAAGTTAAAACTAGAGGATCAAATTAAGTTTGTTGTTGCTTTTGAAGCTAATGAACCGTTAGTGATGATATGTAGTGATGGTAGTGTCATTAACTTAAAACAAACTGAACTAGTTGTAGTTAGCAGGATGGCAACTACAAAAAAACTGCCTGTTAAGAAAGCAATTAACTATTGTTTTAGTGATGCAACTAACACCCAGTTAATTAATTTTCAGGGTAAGAACGGTAGTAAATTAATTACAACTAGTGAACTGAACCAGATGAGTAAAACTGCAATTAGTCAAACCAGGTTTAACAAACTTAATTAGGTTTGGTTGTGGATCTTTTTATCATATTTTTCTATCTCTTCTTTTAGAGTAGTTAATAAGGCTTTAATCTTTTGGTAGTCCATTCTGGTTGGTCCTACTATAGCAAGTTGGTGCTTGGCTTCACTGGTGGTATTGATTAAAGTAGAAGCAACTGATATCTCTTTAAAACCCAATTGATCACCAAAGACAATGTTGGTCTTTTGGTTGGTTTGGTTTATGAATGCCATCTGTTGTCAAACACTAGTATCTTCAAGCAGATTTAAGATTTTAGTGAGTTTCTCTTGGTTAGCAAACTCGGGTTGTTTAGCAAGGTATTGGATCCCATAGATCTTTTTATTTGCCTCAATTTGATCTAAATCAAATAACTTAAATAAGATCTCATCAATTACATACTGATACTCATGAACCTTGGTTCTAATGATCTCTTTTAAAACTTCTAGTTGGTTGTTAATCTCTGAAAAGCGGGTATCAATGATGCGATCATTAAAGATCCTTACACAAATAACAATGTCTTCAAACTGCCTTTGGTTAGCATAGCTAATGGTTTTTTTAAAGACTTTACCACTAGCGGAGACCAATAAAAATAAGGCAAATGACTTATCAAGGATGATCAGATCAATCTTTTTTAAAACTTCATCACTACTAAAGTTAGTTAACACTACTGGTAGGTTAATGATCTCATTGATAAACTTAACCCCAAGTTCAATCACCTCATCAATCGTTTTGTGTTGCTGGAGAATAACACTTCTTAAGCGGGTTTTCAGATCGTTAGTGGTGTTACTAACATTTAACACTTTGACATAATACTGATAACCAATTTGGGAAGGAACACGGCCTGAAGAGATGTGGTTTTTCTTTAAGAACCCTTTTTTCTCTAAAGCTGCCATCTCATTTCGTAATGTTCCCCCAGAGAGGTTTTTAAAATATTTTTTGGTTAGTAATTTACTGCCAACAGGGATAGCATAAGCAATGTATTCATTGATAATTGCTTTGAGAATTTGGGCTTGCCGTGGCGTTAAATTCTTCATTAGTTATCTAATTTTATCTTTTAACAATAACCAGCTTGACCACTAATCTAAAACAAAAGTTAAAAACTGCACCTAAAAAACCGGGGTGTTATTTGTGAAAAGATAGTAACGGTAAAGTTTTATATGTTGGTAAAGCTAGTAATATTTTCAACAGGGTCCACCAGTATTTTCAAAAGAATAATCCTTATAAAACCCAGCTATTATCAAGCCAAATTAGTGATGTTGATTTTTTCATTCTCAAAGATGAAAATGATGCTTTAAATCTGGAAGCAAAGCTCATTAATCAATATCAACCCCGCTTTAACTTAGTTTTAAAACAAAACAATGGTTATCTTTATTTTTATATCACTAAAGCCAAAAAACCTACCTTGGAATTAGCCAGAAAATACCAGATTAAAACCACTAAGTGCTTTGGACCGTTTGCTTCAAGTAAGTTTAAGTTACGTGAGATCCATGACCTACTTTTAAAACTCTTTCCTTTAAGAAAGTGTGCACCTCACCAAAAAAACCACCCGTGCTTTTATTTTCAGATGGGTTTATGTATGGGGCAATGTATGCAAACTGATACTAAGGAAAAATACCAACAGGTAATTAGTAACATTGAACAGTTTTTTAATGACCCTAGTGTGGTAATTAACTATTTAAAAGCTGCAGAAAAAAAGGCAAGTGATAATCAGGAATTTGAAAAGGCCCAGCAGTTTCTAACACTGCAAAAAGCAGTTTTAGAGTTAACAAAAACCCACCATACCACTATCATTAAACAAAAATCAAGCCATGATTTTATTGGGTATGTCTTTCAAAATAACGTTTTGGCCATTACCATTTTTTGTTATGAAAAAGGGGAGTTAACTGATAAAGAACAAGCAGTGTTTACCCTAGAGCAAACTGACATTGTGGAAGTTGAAAGTGCTATTATCACCTTTATCTACCACCACTATAAAACTACCCCACTTCCAAGTAAGATTACTGTTTCACTTGATGAAACTAACCTAAAACTTATTAGTGATAGCTTAAAAATTGGTGTTTTTAAGCCCAAGGATGGTAATGAAAAACTGATCTTACAAACTGTTATTGATAATGCCAAACATGCACTTGCAACCAAGTGGTTGAAGTTTACTAGTAACTATGATAAAACCCAGCTCCACAAGGATTTAGCACAACTTCTAAATACTGATTATATCCATAGTCTTGAGATTATTGATGTGTCATTCTATGATCAAAACCATGTTGTTGGTTGCATGTTAAGGTTTGAAGATGGTAAAAAGATCAAACACTTATCAAGAAGATACAACATTAACAGTTTAAAAAAAGGTGATACTAACCACATTGCTTTACTTGTTTACAGAAGGATCTTAAGTGCGATGCAAACCAAAGCTAACCTCCCTTTTAGTGATCTTTTAATTATTGATGGTGGTAAAGCACAAATTAAAAGTGTTAAGCAAGTTTTTAGTCTCTTCAGTAATGTTAAACCACCCATTATCATTGGACTAGTTAAAAACAAAAACCACCAAACTGATCACATTATGTTATCTGATTTCCAAGTTAAAAAGATAGCAATTAACTCCCCACTCTTTCACTATTTAGCAACAATCCAAACTGAAGTTGATGGTTTTGCTAAAAGAAGTGCTTTTAATAAGTTAAGTAACCACCAACTGCAAAACCCGTTGCTACAAATCCCAGGAGTTGGCAAGATAACTGCCCAAATTCTCTTTGATAACTTTCAAACGCTCAATAACATAAAATTAGCTTCAGTTAATGAGTTAAGCCAGTTTATTAAAAAACCATTAGCACAAAAGATTAAAACTTACTTTGCAAAACAAACTGATTAAGGTTTTGGTAATTGCTGATACCCATGGTCAAAACCAGAGGTGGATTGAACTGAAAAACTACCATAACCCTGATGTGATTATCCATGCAGGAGATCACATGACCGACCACTAAACAATTCATGGATCAAAATGCTACTTTTTGAGTGGCAGGTAACAACGATTCAATTGGCAATGAAATTGAAATTTTCCAGTTAGGGCAAATTAACTTTGTGTTAATGCATGGTCACCAAGCACCAAGAGATAACCTGAAAAAGTGGTACCAATTATTAGTTTTAAAAGCACAACAATACCCTTGTGATGTTTTAATCTTTGGTCATAGTCACATTGAATATACCAACAAAATTAATAAGATCCAGTTAATCAACCCTGGTTCTCTACAACTACCAAGAAACCAAACCAACACCCCTTCATACTGTACCTTTATTGTCAATAAAGACGAGCTAACTGATCTAACTATCCACTATTACCAAGCTTCAAAAGTTAGTTAACAGGATCTTTTAGATAGATAGGTTGTAAGGTTAGAGGATCTTCGATTCTTTCAAAGTGATCGATGTTGCTTAATAACAGCTTGCTATAACTCTCAATGTTTTCAAAGTTTTCATACATAGGAAGTTCATTGTTTGCCTTACATAACTTCACAAAATCAGCTTTACTAATTAGCTTTATTTCACTAGTAGTTTGGCTGTATAGTCCACAATAGTTCTGATCATTACCACAACTAATCTTGCTAATCCCATGTTCATATGGGATTTGAAAGCGGAGTGAATTTAAAGCATAAAGTTCACAACTGGGATACAATAAACACCAGCTTTTAGCAATGATAGTTGCTATCCTCTGACCGGTGAAACTACCAGGGCCAATTGTGACATAAAACTGTTTAATACTGCTTTTTTTCAACTTGTTTTTTGTTAACATTGTCTCCAGATAATAAACAGCAAGTTCAGTTAAGTTCTGTTCAACACCAATGGACAACTCATCAACCACTGCATTGGTTTTCATCTCTAAAATAATGATGTTAAGGGTTTTATAAGCACAGTCTAAAAACAGCTTATACTTACTTAAAAAGAACATGGAGTGGATCGGTTAAAGATAAGTTTAAACTCTTAAGTTTCGTTGAAAACTTAGGTTCAAGTGGGGCGTGAAAGTCCATAGGTTTGTTTAAAGTGGGATGGATAAAACAGATCCTGTTTGCATGGAGAAACTGACCATAGCTATCTTTCTTTTCACTTTTAATTCCATACAGTGGATCATTATAAACTGGATGTTGGATAAATTTCAGATGCACTCTAATTTGGTGGGTTCTACCTGTTAACAATTCCAAGCTAATTAGTGCTGCTTTTTCATTCTGATTAATCACTTTAAACTTAGTTATTGCTTGCTTTGCTTTAGCAGTTTGGGCTATTTTAAACATTACCTTGTTGTTATTAACCCTTGCTAAAGGTGCATTAATTGAACCAGTTAAGGCATTAAAAGGGAAGTGGACTAGTGCTACATAATAACGTTTTAAGGTGCGATTTTGCAGTTGATTTTGCAAATTTAATAAGCTTGCTTGGTTTTTACAGACAACAATTGCCCCACTAGTATCACGGTCCAATCTGTGCACTAAGTAAACAGGGTTTTTGTTGTTGTGAAAGATACAAGCAGCTAACAAGCTGGCTTTTTCATTGAAAGTGGTGGGATGGGTTAACAAACCTGATGGTTTGTTAATAACCATCAAATCCTTGTCTTCAAAAAGAACCTCAAGCTTTAAGTTATAAGGTTCAACACTAGTAATGAAATCACTAGTTGTCTCATCATGAATCTCAACTTTAATTACATCATCTTTTGCAACTATTAAACTGTTTTTAAAAGTTAGTTTTTCATTAACTTTAATCTGTCCATTCATGATCAGCTTCACTACCTTTACTCTTGAAAGGTTCAGTAAGCTAGCTAAAAGACTATCTAAGCGTTTGGTAGTTGTAACAACAAAACACTGTTTCATTACTGGTTTTTGGAATGTTTAAACTCTTTGAACATCTGGATTAAAAAACAAAAGAATAAACCTATAAAACCAAAGGTAATACAACAATCAGCAAAGTTAAATACTGAACTACCATTCTGAAAGATAAAGTAATCTAACACTGAATCATTAGCTGAAGTTAAACGATCAAAGAAGTTTCCAAGTGAACCAAATGCTAAAGTTGTAATTCAAAAGATGTAACTATATTTCACCATAAAAACAAGAAAAACTAACGCAATTACTGATAATAATCCCTGGAGAAAGTAAACTAAGCCAGTTTGGTTTTGTAATAAGCTAAACCCTACCCCTTTGTTTCTAATCACATAGATATTAATAAAACCACTATTTGCTACCATGGTGTTATCCATCTCACCATTTAACGCATTTCTTAATATGAAAACTTGCAGTAAGATAATAAAGCCAACAAACCCAATCATTGTCAGTTTATAGAATAAAAATGGTTTTTGGTTTGCAGTTAAAACCTGGTGTTTAAGTTGTGAAAAAAACTTGGTTTTTCTTAATTTCATCTACTTGTATATTCAGTTAAAATTCACTAATTAACTAACAAAAATTATTAAGGAGTATTAACACTTTTTTACTAGTTTTTAAACTAACTTTTTTAAGTGTAATCCTAGTTTAATTTCTGGTAGTTTTCTTTACTAATAGCTACTTGTAAAAAATTAAAAGTTAGAATTAATGGCACTATTTAGTATATAGATATCAGGCTTTGGGGAAAACAAAAAACAAATCAGACTGACAGATCTTTCTAGAAGATTACCGTTTTTATTTTGAAACAGATTTTGATTGGGTTACATACCTGAACAACTGTTTAAACAGCTATCCTGATTTTGACATCATCAAGTTCATTAAGAAGTATGGCCCTGAGTGTGAAAAGAGCTTTCTAAGCTGACAGAGCAAAGCTAAGAGTGATGTTTACAGTGAACTGACTAACAAGATTAAAAAACAACAGTTCTCAGAACAGTTAATTTACCAGCTAGTCCAACTTGATGCTTTACGAACTAACTATTTAATTGGTTCGTTGTTTTCAGATAACAAAACCCAGCGCAAACTCCTGAAGCGTTCTTGAAAAAATGCTAAGAAAGAAGGTTATACAAAACAAGAATGGTTGATGATCTTAGTTGGTTTACCCTTTGAAAAAGGTGCTTATCATAAGCAGTTATATGACCATTCACGTCAGGAGATCTTAGATCTTACTGAAGTTATTAAAAAGCTTTATCTAAAAACAGAGACCAACAACGATAAGCTTGAGTTTGCTGCAACTACTAGTAAAACAACAGCGCAGCTAACTAAAACTATGCCCTTAAACAGTAGTGATCTTGATAAGGATCTAATGGAGTTTAGTGGTGAGAAGTGAGGTGATAATTAGTGCCTAAGATTGAAGTTAAAAATGATGATTTAGAGCTAGCTTTAAAAAAGTTTAAAAGGATATCACTGGAAGTACGCAGGTTAGCACAACGCCATGAATACCACTTGCGCAAAGGGATGCGGTTAAGAGAAAAACAAAAGATAGCACAGAAAAAGCGCAGGAAGTTTCGCAGTTTAGCTAGCCATTAAGATGGATAATAAAAACCCCCAGAAACTTATTACTAGTGAATTGTTGGCAAACCACCGCTTTAATTTTGCTAAAGATGATAAAGGTGGGTATGATGCTAATGAAGTTGATGCATTCTTAGATCAACTAACCAAGACTTTAATCCACTATGAGGAGATGAAAAACAACGAACAAGAATTGAAAAATGCTTATGACAAGTTGTTTTCAGATCGTGATCAGATTTTAAGTCGTTGTGCTAAATTAGAAGCTGATTTAAACACCTTTTATGAAAATGGTTATGCAAACAAGGTGTTAATTAACCGGGTTCAGGAGTTGGAGGATAAACTTGAAAAACTACCTGATCGTTACACTGAAAAACTAGAAAGGATTGAAAAACTGTTAAAAAAGGTCATTAAACACTGAACTGATGGGGAGGACATTAGTAACTTTGAAGATGAGTTCTTTTAAAGATGGTTGTTGGGATAGGGATTGATGTTGTGCAATTAAAGCGCTTCTTAACTTTAGTTGAAACTAGTGATTGTTTTGCTAAACGATTGTTAACTAGCAATGAACTAAACAGTTATTGAAAGCTAAACAATAACCAAAGAGCTAATTTTCTAGCAGTGCATTGAACTTTAAAAGAAGCGATTTATAAAGCTACCAGTCACATCAAACCACTTTTCACTAAACTTGAAATTTATAAACTTAACAACCAGTACCGGTGTGAATTTATCCAAAACATCAACCTGTTGTTATCAGTTAGTTACACTAATTGCCATGTTAGTGCTATCTGTTTAGCACAACAAAATGGATAAACTATTTAAAACAAGTTTTAGATTCATAATAAGGTTTTTACAAATCCTGAGTTTACCAGTTGTTTTTCCTTACTTTTTATTAAGCTTTTTAGCTTGTTTAATTACTAGTAAAAACTATGAATCACTCCCTTATAACTATCCCCCTGAAATCCGGTTCAAAAAGGTGTATAGATTGGTATCAATGTGACTTTACATTAAGGGAATTAAAGTAGTGACAGTAAATGACAAGATTATCCCTAAAAAACCAGTTTTAGTGGTAGCTAACCACAAATCTAACCTTGATCCTTTAGTATTAATTAAGGCCTTTGGCAGGTTGAAAAATAGTCCACCATTAACCTTTGTTGCTAAGATTGAACTGAAAGATACAGTCCTTTTTAAACTGATGAAATTAATTGATTGTGTTTTTATTGATCGAAAAAACATCAGACAAATTGCCAATGCATTGGAAACCCAACAACAACTAATTCGCCAGGGCACTGCTATTGCTGTTTTTGCTGAAGGGACTAGGATTTTAAGTAATGACATTGGGGAATTTAAACCAGGAGCACTAAAGGTTGCTTACAATGCTTTTGTACCTATCTTACCAGTTAGTATTGTGGGTAGCTTAGGAAAGATGGAATCAAACAAAAGGCTAAAAGAACATGGTGTTAAGAAAAGTTCAAACTATGAGGTTAAAGTAATCTTTAACAAGCTAATTAACCCAATTAGTTTTAACCAGATTGATTCTAATAACCTTGCTAATAACATTAGAAGCATTATTAGTGATGCATACACTAGTGAAAAACCAAGCAATGATTAGTTTAATTTTTGATAAAAGCAACAGTTTTAACCCCCAACTTTGTGTTGAACTTCTCAACCACTTTAAAGTGGTTATGCTAACTAAAACCATCGTTGTTGACTATCCCAGCTTTCAAGCATGAAAAGCACAACTCAAACCGTTTAAATTAGCAGTTTTTAGTGATAATTTGCAAACCGAATTAACCCCAAATTCAAAACTCACAGTTTTTAACAACTACCAGCAACTTTTAGTTGATAATAATGACCTAATTATCTTTGCAACCCCCACTTTAGTTCAGCTTTTTGATAATGAGATTGACCAGTTAATAGTGATCAATCCCACTAGTAAATCTAAAGATCAGTTTAACTGTAATTGAAACGACTTTGTTTTAATTAAACAAACTAATTTCAAGAACCATCAAGTTGGTTATTTTGATAAAAAAAGTAGTTATGAACCACCTTTCACTGTTGTTACAGACCACTTCTTTGGGAACCTAACTGATTTATTTAGTCTTTTAGTTGATAAAAAGGTAAGTGTAAATGATGTGGATATTGGCTTGATTTCCTTACAATATCTCAATATCATTGCAAATTACACCAATAAAAAAGCCATTGAAAAGATCACTGATTATCTGGTGATTACAAGCAAAATTCTCGCTAAAAAAGCAGATAATCTGCTCAATGATCATCAGGAAGAGAGTGTGTTGGAATATGATTTAGCTACTAATAACTTTCGTGATAAGATGATCGCTAACTTAGTGGAACACAAACGCTATTGTGATTCACTGGGTGAATTTGAAAAACTCAGGGTTAACCGCTTAGCTTACTTTTCCAAAGCTAATGAGATGGAGCAATTTATCAAAACAGCTAATGATCAACTTGTTACAGTTGAAGACCAACTCCCTAACTATATTAGTGTCTTAAAACTCTTTCATGCTATGAACAAGTTACTGGAGATGAGACTCAGCTCTCTTCTAACAAATAAAAACATCACCATTAAAGAGTTATCAGTTGAACAGGTTCAAAAGGAGTTGGTTTTAGCAATCAAACAGTTTAACTACCAAACAGTTTCTTTAAAACGGGTGTTATTAAAACTTAACCATCCTATCTCTTTAATGTATTTTGTTACTGCTTTTGTGGCACTTTTAGTGCTATTAAACAACCAGGTAATAGGTTTGGAACAAAAAGATTATCACAGTGAACTTTACATCTTTTTACTAGATGAAAACCAACTGAAAACCTTCCAAGAATCACCAGATGAAATGGTAAAAAGAATCCAAGCTCAACAACAGCAAAACGAACTGATAATTGCTAAAAACAAGCAACTAAGAGCTATCAAAAACAAACAAAAGCGAGCTGATTATCTAAAAAAGAAGTATGGTGAAAATTACTTAGATAAAACTAACTTAAAAGATGAAAACAACAATTAATATTGCCACCCCTACCCTAAAAAAACCTAGCAAAGAAGCTAACTTGGTTGCTAGTATCTATGGGTTGTTATTTGTTTGTGGCGCGAAAGGGATCACTTTAAGAGAACTAATTAGGATCTTTAAAAAAGCAGGGATTGAAAAGGTGAAATTAGCACTCTTAGCACTTGAAAGGAAGTTAGCAGATGATGAGCAATCAGGAGTGGAGTTGAAAAAATTTGGTAATAGTTTTTCTTTGGTAACAAAACCAATTATCAAAGACTATCTCCACTTATTATTGGCTCATAAAGTCAAAAATCCCCTTAATTCCAAAGCAATGGAAGTGTTGGCTATCATTGCTTACAACCAACCTTGCACCAGACCCAGAATTAATGAAATTAGGGGAGTTGATTCTTTTCAAATTGTTGATGATCTAATAGCAAAGGAGTTAATTGTGGAGTTAGGGAGAACTGATAAACCAGGTCGACCTTTTATTTATGAAGTGTCAGCTAAGTTCTATGATTTATTTGGCATTGATAGCTTAGATCAACTCCCTAAGATTGAGCATTTTGATCTTGATAAATTTAAGCAAGGTAGCTTTTTTGATTCCAACCGCTATGGTGATGAATAACCTTATATAATTTACAACATGGATAAAATAGCTATTTTAACTTCGGGTGGTGATGCTAGTGGGATGAATGCCACCATCGCTTATCTAACCAAATATGCAATTGCAAAGCAATTGGAAGTTTTTTATGTAAAAAACGGTTATTATGGCTTGTATCACAACCATTTTATCACCAGTAAGGAACTTGATTTAACTGACTTTTTCTTTATGGGGGGAACAGTAATAGGATCAAGTCGTTTCAAACAGTTTCAAGATCCTAGCTTACGAAAACAAGCAGTTTTAAACCTCAAAAAACGTGGTATTAACAACCTTGTTGTTATTGGTGGGGATGGGAGTTATATGGGTGCTAAAGCACTCAGTGAATTAGGATTAAACTGCTTTTGTTTACCTGGTACGATCGACAATGATGTCAATTCCAGTGAATTTACCATTGGTTTTTGAACTGCTTTAGAAGCAATTCGGGTTAATGTTGAAGCAATTTATCACACCACCAAATCCCATAACCGCTTAGCAATCATAGAAGTGATGGGGCGTGATTGTAGTGATCTGACCATCTTTGGGGGGTTAGCTACTAATGCTAGTTTTGTTGTTACTAGCAAAAATAGCTTGGATCTCAATGGCTTTGAAAAAGCAGTGAGAAAGGTGTTGCAATTCCAGAACTATTGTGTTGTTTTGGTTAGTGAAAACATCTATGGTAAGAACGGTTTACCTAGTTTAGAAATGGTTAAAGAGCACTTTGAAAACAACGCAATTAAGTGTAACCTAGTTTCACTAGGACACACCCAAAGGGGCTTTAGTCCTAATAGTATCGAACTCTTTCAGATTAGTTTAATGGCTAAACACACGATTGATCTGGTTGTAAATAATGCCAACAGTCAAGTAATAGGGATGAAAAACAACCAAGCAGTTAACTATGATTTTAACACTGCTTTTAATTTACCAAAAGCTGATAGAACCAAGTTACTTAACCAAGTTAACACTGCAATTATTTAACGATGATTGACCATTTAAAAAGAACAAAGATAATCGCTACCTGTGGCCCAGCTTTAACAAAAAGCTTGGTTAGCTTAAAGATGCTTGATGATAATGAGTATGCAGCTATTAAAAAGGTTGCTTATGCCAACATTGAAGCAATTATTAAAAGTGGGGTTAGTGTGATTAGGCTTAACTTCTCTCATGGTACCCATGAAGAACAACAAGTGAGGATCAAGATAGTAAGGGATGTAGCGAAAGCAATGAACATCCCTGTTTCTATTATGTTAGATACAAATGGTCCTGAGATCAGGATAGTAGAAACTAAAAAAGAGGGTTTGAAAATCACCAAAGATAGTGAAGTGATTATCAACACCATGAGTAAAATGATCGCTAGTGACAACCAGTTTGCTGTCAGTGATGCTAGTGGCAAATACAACATGGTTAATGATGTGAATATAGGTCAGAAAATCCTTGTTGATGATGGTAAGTTAACCCTGGTTGTCACAAGGGTTGACAAACAACATAACCAGGTTATCTGTGTTGCAAAAAACGACCACACAGTTTTCACTAAAAAAAGACTTAACCTACCCAACGCACAGTACTCTATCCCTTTTCTCAGTGAAAAGGATCTGAAGGATATTGACTTTGGTTTAAGCCAAGGTATTGACTATATTGCTGCCTCTTTTGTTAATACTGTTGCAGATATTAAACAACTGAGAGATTATCTGAAATTAAAGAATGCTAGTGGGGTGAAGATCATCGCTAAGATTGAATCTAATCATGCTTTAAATAACATTGATAAGATCATTAAAGCTAGCGATGGGATTATGGTTGCTAGGGGTGATTTGGGCCTTGAAATCCCTTATTACCAAGTCCCTTACTGACAAAGGTACATGATTAAAGCTTGTCGCTTTTTTAACAAGCGTTCTATTACTGCAACCCAAATGCTTGATTCACTAGAAAAAAACATCCAACCAACCCGAGCTGAAGTGACTGATGTTTACTTTGCAGTTGATCGGGGTAATGATGCAACTATGTTAAGTGGGGAAACTGCTAGTGGGCTTTACCCTTTAAATGCAGTAGCGGTGATGCAAAAGATTGATAAACAATCAGAAACCTTCTTTGATTACCAGTATAACGTTAACTATTATTTGAAAAACTCCACGGCAAATAAAAGTAGGTTTTGACACAACGTTGTTTTACCTTTAACAAAAAAGACTGTTCCTAAAAGAAAACTTGTTAACAGTGCCTTTAAGTATGACTTTATTGTCTATCCTACTAATAACATTAACAGGATCTATGCATTATCAAACGCACGCTTAGCAGCAGCAGTTATTATTTTAACCAACAACAAACGGGTTTACACTGGCCATGGTGTTGATTATGGGATCTTCTGTTATTTAATTGATAAAAACCCCAACCAGCTAACCAAAGCTGAACTGATTGAACTTGCTTGGAAAGCAATTAACCACTATCAAGCTTATGGTGATTTAGAAAAACTCAAACAGTGTTTAGCTGTCTATAATGAAACAATTATCAATCTTTAGATAAAAATTGGTGAGAGTTTGTAAAATATTGTACACGGTGCCTTAGCCAAGTGGACTCAAGGCCTGGAGCTGCAACCTCCATATCGTCAGTTCGAATCTGACAGGCACCTCCATGTACATGAAAGTTAACTACTCTCAATGTGCAAACGGGAAGTAGCTTAGTTTGGTAGAGCACTTGGTTTGGGACCAAGGGGTCGCAGGTTCAAATCCTGTCTTCCCGACCAAAAGGCTGGATACCTCAGTTGGTTAGAGGGCCCGGTTCATACCCGGGTTGTCGTGAGTTCGAGTCTCACTCCAGCCACCAAAGTTACTTAAATATTAAAGGATCTATAGCTCAATTGGTTAGAGCCCCCGACTCATAATCGGTCTGTTACAGGTTCAAGTCCTGTTAGATCCACCACTTTGCTGTGTTAGAATCATATTTGCCGCAATTTTGTGGAGACTTACCCAAGCGGCTGAAGGGTTCGGTCTTGAAAACCGAGAGGTGCTTTATAAGCACGCGAGGGTTCAAATCCCTCAGTCTCCGCCAAATAATATTTAATCGCGGGATAGAGCAGTTGGTCAGCTCGTCAGGCTCATAATCTGAAGGTCGAGGGTTCAAATCCCTCTCCCGCAACCATGGTTCCATGGTGTAGTGATAACATATCTCCCTGTCACGGAGGGGTTGCGGGTTTGATTCCCGTTGGAACCGCCATTGGTCTTGTAGCTCAGTCGGTAGAGCAACGGTCTGAAGAACCGTGTGTCGGCAGTTCGATTCTGCCCGAGACCACCATTAAAAGTTTTTAAAAAAGCCCTAAAAAGGGGCTTTTTTAGTGTTAACACCAATTAAAATATTTTTATAGCAAGCAGTTGCATATTTTTAATATAAGTTAGAATTATTGGTATAGTGTCTTCAGCTGTTTAATTCATATTAAAGCGCATGGAAAAAAATAGATCAGCTTTTCAACAAAACCAACAAGCATCAAACCAACCTTTTAACCAAGATCAAAACCAGTATTACCAAGATCCTAACCAACAACAATTTAACCAATCTGGTTTTGATCCAAATCAACAGCAATTTAATCAACCAGGATTTGATCCTAACCAACAGTATTACCAAGATCCCAATCAACAACAATTTAATCAATCAGGTTTTGATCCAAACCAACAATATTATCAAGATCCCAATCAACAACAATTTAATCAATCAGGTTTTGATCCAAACCAACAATATTATCAAGATCCCAATCAACAACAATTTAATCAATCAGGTTTTGATCAAAACCAGTATTATCAAGATCCCAATCAACAACAATTTAATCAATCAGGTTTTGATCAAAACCAGTACTATCAAGATCCTAATCAACAACAATTTAACCAGCCTAGTTTTGATCTAAATAACCAACAATTTAACCAACCTGGATTCAACCAATCCCCAGCATTTGAAATCACACCTCAAGAGCAAAAAGCTGAACAGGAGATGTTTGGTGAAGAACCACCTCAAGTAGTTAGAGAGATCCATGAACTACCATTTGAAAAGATCCGTTCTTTTTTACAAAGTGATTTTGACAGCTATAACTTTCGCTTAAACTCACTTAAAAGCAAACTGGATAATGCCTTGTATTCACTTGATAAAACAATCCAAAATACCAATGAAAACACTGCTAATCTAGAAGCAATTAGACATAACTTAGAACAAAAGATTCAAAACCAAAGCAAGCAATTAAGAACTAACTTTGATACCCAAAAGCTTGATGATAAGATCAATGAATTGGAGATCAGAATGCAAAAACTAACCAGGAATTTTGAATCTCTAAGTGAACTTTCAAAGCACAACTCTTATCCTAATTACTATGAAAAATTGTTACCAAATGGTGGTGATAGTATGACCAATGTCTTTGAAAAAGCACTAATGATGAATTTATTGAGAACTACATTACCCCCTCAACCCCAAGTTCAATACTACCCCCAACCCTATCCATACATAAGACCTTACTATGATGAACCTATTTACGCTGGGTTTAGAAGAAGGGGTTACCGTGATGACTTCTATGAATAAAAAGCGTGTTTTAACTAATGAAACCATTTGATAAAAAACCTTCGCTCCAACCAATTTATGACATTGGTTTTGATGATGGTTATCTCCAAAGTGAGTATGAAAAAAATCGTTCTAAAACCGATGTTGATAAGATCGAAAGCCAGCTTTTAAAAGAGATTAAAAGTCTGGAGGATGAACTTAAAAACCTCAAGGGCTTGAAGAATCAAGCAGAAGATAATCCTGAACTTGATAAAAAGATTAACCACTTGGAAGTTGATCTAAACCGTTTGGTTAATGAATATAAAAACTTCCAGTTCCAAAAGAACCACATGGTTGATAAGGTTAGTGAACTTGATAACCTAACCCGTTTTTATAAGAATGAACTAACCCGTTTGCAACAAGAAAACGCTGATTTTCTCAACTCCAAGTATGCTAATTTAGCTAACTTCCAAGCTAACTACCACAATAAACTAAATGATTTTCACCGCTTAATAGAAAATCAAAACCACACCATTAACCGCTTAAACCAAAAGATTAATGGTAACCAAAATCTGATTGATAATAACGTTGCTTTACTGCAAAACCCCAACATCACAGTTGAAAAAAAGAACTACTTACTAAATGTTATTGATCAACTTTACAATGAGCTTGATCAACTTGAGAATCAAAAAAGATTATTAAGTATTGAGTATGAAAATACCTATAGAGAGTTAGTTAGTGCAGATAATGAACTGCAAAATGTTTATGAAAACATCGATCAAAATCAGATCCAGTTTAAACACCAATACCAAACTTATAGAGATGAGTTAAGTCAACTTGAGCGCAAGATCCAGCTCACCAAACAAGAGTTAGTTGATAAAGAATCAGCACTAAGAGTAAAGATAGATGATGCTGATTTTTACATTAATGCCCGTTTAGCTGAACTTGATGATGTAGCTAAACAACTTAGCTTTCAAGATGGTATTACCAAGCAAAATGCTCAACATGTTGAGGATAAGTTAGTTGCTTTAAACAAAGAAAAAGACCGTTTAAATACCCAAAAAGAGGCCTTTTTTAACTTAAGACAATCTGCTTTAATTGATATCAATAAACTCCAGCAGGAAAATGAACTGTTTGCTAAGCACTTAGAACACCAGCAAAATGAGTTTGAACAAAAACAGTCTGATAGCCTCTTAAAGCTTGAAACTGAATATAAGGCTTTACAACACAAGATTAATGAATTTAAAAATGAAAGTGCCACTAAGAGCGAGGAACTTTTAAACCAAGAACGGGAACTATTTGAAAAACGCAGGGAAATTGACACGCTTTTAACCCAAGCATCTTTGGAATATGAACACCAACGTGAGTCAAGTCAACTACTCAAAGATAAGCAGAATGAAGTAAAACAACACTTCCAAAACTTAGAGTATGCTAAAAAGGAGCTAGACAAAGAAAGAAACCTCTTAGATCAACAAAAAAAAGTTGATAGTGAAGCAATCTTTCAACTCAAAGAAAAGGTTGCTCAAGAACGTAAGGAGCTTGAAGAACTATACCTTGTTAAAAAACAAAAACAAGATCAGAAGGAAAATGAACTGTTGTTTTTTGAAAAGCAGTTAAAACAACACCAAGCAGATTTTGAAAATGAATTGGAAGCTAAACAACAGGAGTTGTTTGAAGCTAAACATGCATTGGAACGTTCCTTTATCAAACTTGAAGATAAAGAAAAAGATCTTAACACTAAAGCACAACAGATTGCCAATGAGTTTTCCCAACTAAAAACTGATAAGTCAAAGAGTGCTGATTTTGAACTAATGTTGCAAAATGAGTATGAAAACTTGCAACAAGAAAAACAAAAGTTATTCCAAGAACGTACTTACTTTGAAAGGAATGCTGCGGTTTTATCAAACCGGTTACAACAAAAACGTGAGGAGTTATTACAACAAAAAGAAACGCTTGATCAGCTTACAAAAAGCTTTGAGCAAGAACGGTTAATCAACCAAAGGGAACACAAGGAGTTGGTTGCATCAGTTGAAAAACAAAAGGAGATATTGGGCAAAAAACTCCAAGATTTTTCCCAAACTTCACTGAACGCTTCTAAAAATTTAGCTGAACGGGAGATGGCAATCAAGTTTAAAGAAAAGGAGATAGAAGCAACTGAAAAGCAACTGTTAAATGATGTTAATAATGCTGAAGTTATCCAAGCAGACTTAGCACAACTCAACCAATCACTTAACCAAGAACGCAGTGAATTGCAAAACGCCAAACAAAGGATTGCTGATTTTCACAATGATTCACTAAAAAAACTCAATGAGTATGAACTTAGCTTACAAAAACGGTTGCAAGAATTACAAACCCTTGAGGTTAACCAAAAACAACATTCATATCAAAATCAAGCTTACTTTGAAGGTGAACTTGATAAACTTAACAGAGAAAAACAAGCTTTTTTGAACTTACGTAAGAAACAAACTATGGAGGTTGATGCTATTAAACAAAGGTTGAGTGATAAACATCAAGCTTTAAATATGCAACAAGCAGAGCTAGATAGAAAAACCCATGAGTTAAATAATGCTTTTTTAAACCATGATGCGGATCAAAAGAGTCTACAGGACCAACTAGCAACTGTTAAAGAGACCCAAAAACTAATTGATTTAGAACGTAGTGCACTGCTTGAAAAGCAACGTGAGTTTGCTGAAAATGTTGCTGGTTTTAAGCGCCATTGGTCTAATAAAACTAGTCAACTCCAAAAGATTTATGAACTGACCAAAAAACAGGAAAGTGAGCAAACCCAAAAGGAAACAGAACTAAAGATTGCTTTTAGTGATCTACAAAAAGACTATCAGGTTTTTGAACTCCAAAAGGACCAAGAATTTAGACAAATTGAAGCTAAGCAACGTGAACTTGACAAGTTAGCTGAAAAAAATAATCAGGTCAAACTAGAACTTGATAACAGGTTTCAAGCGCTGCAAAACCAAAAGCAAGACACAGTACAAGCTCAGCTAGAACTGGAACGTGAACAACACCAGTTAAACCTTGAGCAAACTGCTTTCAACCAAGCTAATGAATCGCTTTTAAAACAACGTGAACAACTCACCAAAAAGATCCAAGCTTTCCACTATGAGTTGAAAAAGCGTAACCAATTCTTAGCTTTAAAAGGGAAAAGGTTGTTTGCAAAAGAGCAAGATCAACAACGCAAAGATCAGGAGATCAACTGACGCTTTAAACAGTTTGAAAAGGAATATACTGATTTTGATGAAGCTAAGAAAAGGGAACTTGAAGAGCTTGAAAAGATCAGAAGAAGTTTAAGTCAAAGCAACGTTGAATTAGAGAGAAAAAGAGAAAAACTGGCTACTGATTTCACTAATTTAAATAAGGTTCAACACAACACCCAAATTAACCGTGATCAACTTAACAGTCAGATCAGACAGTTCTTATTAGAACGCAAAAACTTCCAACGCTTTAGTAATGAAGCTAATGCTAAAAAAGCCTTTTTAATTAAGCGCTTAAGAAGCTTTGCATCCAATCTAAAACTCCAAAAAGAAGCGTTAGCAATCCAAAAACTAGAGTTTGATAAGCGTGATGAACAACAGAAAAAAGAGTTACAGCAAGCTACTTTACAACTAGAACAGTTCAAGTTTGAAAAGCAAAACTTTGACATTGAAAAACAACGCCAACTAGTTGCTATTAAAACTCAGTGTGAAAAACTTAGTGATGAAAAAAAGGCACTAAACCAAAAGCTAGTTGAACTAAAAAACTTATCCCAAACCTATCTTGCTAATAAGAATAAGGCTGAATACTCCCAGCAACAACTCCAACAGAAATACACCAATTTACTTGATCTGAAGGAAAACTTAGAGAGAACCAAAGATCAATTAGATAAAAAACATCGTTCTATCTTCGCTAGATTAACTAAGTTTGCAAATGACTTACGTTTTGAAAAAAAGCAACTGTTAAAAGCACAGCGCATAGTTGATGATAAAAACCGTCTTTTGAAAGAAAATGAACGTAACCTCCATTTCCTTTCCAATGAAACAGAACGAAAACGAGCAGTTCTCGAAGATCAAATTTCTTACTTTGAAAAACAACGTAAACAAGCTACTGATGCGATCCTAGCATCACATAAAGAAGTTAAAAAGAAGGAAGGTGAACTGCAAAAGTTACTGGTTGAATTAGAAACAAGAAAAACCAAACTCAACAATGATTTTGCAAAATTCTCAAGACAACGTGAAGAGTTTGAAAACCAACGCTTAAAGCTCTTGGAACTGCAAAAAACCCTGCAAACCCAAACTAATTCCAACAACTTTAAAACCAAAGCAATCCAAGAGATTGAAAACAGTTATAAAAGGGGGATGGAAGAACTTAACTTCCAAAAGAAGGAGTTTGATAAGAATAAATCACGCTTATATGAATACTTTAGAAAGATGCGTGATGAGATTGAAAGAAAGGAAAGTCAGGTTAAGTTAGTTTTAAAAGAGACCCAAAGGAAAGCCAACCTCTTAGAAGCACAAGCCAACAAACTTAACATTGAAAAAAACACTATTGACTTTAAAGAAAAAGAGTTAAAAGCCTTTAAAGATAAGGTTGATCAAGACATTGATTCAACCAATAAACAACGCAAGGAGTTAAATGAGCTTTTAAATGAAAACAAGTTATTACAACAATCACTAATCGAAAGAGAAAGGGCTATTAATTCCAAAGATTCACTTCTAAATAAGAAGATAGAAACGATTAAACGCCAACTCCATGATAAGGAGATGAGGGTGTTGCGCTTGGTTGATAGAATGAAATTAGCAGAACAGAAATACCAAACAGAAATCAACCGCTTAAGAACCCAAACTTTCGATTCTGAAAAACAAGACATTAAAAACTTCTTCCCACCGTTATTTAAAATTAACGGTAATGATATGGCCTTTCCTTACTTATACCCCTGACTATATCCTCAACAAAAGCAAGATGATAACACTCTGCAAATTCGTCAGCTTTTTGAACAGCAGCTGCAGTTCATGCAACAACGCTATGAAAATGAGTTAAATGAATTGCGTAGACAACGTAATTTACTTGAGAAAAAACTTGATCAAATCCAACTAGAATCCCAGCTTAACAATAAGCAAAGTGAGTTTAGTAAGGTGGAATCAATGATGGAAAAACTACTTGAAAAAACTGAGAGTAGGTTAAATGATTTTGATCAGAAAATTAACTATCTCACCAAGAAAGTTAACCAACACAACACCTATCAACCAAGTTCCTATCAACCAACGCCTTCTTATCAAGACAGTGATAAACAACAGCTGTTATTTAGAATCCAAGAACTGGAAAAACAAAACTTATTCCAACAACAATTTCAACCTGCACCAGCTGTTGTCCAACAACCTACCAGTTTTGCAGCCCCTAACATCACCAAACAACAGCAAATTGCCCAACTTAATGCTGAAATTAACAACATTAAAAGGTTGATTGCCCAAAAAGCAGCAAGTAAATAAAGATGGTTAATAATGAATATCAACAACTAAACACTTTAGTTGAGAGTGATGATGAAGCGGATCTTGTGATTGCTAACCTAGTTAAACAACTCAATGAACTAAAGCAAATCCTTGTTTCACTAGACAATCAAGAAGCAAGTGCCACTGCAGTTACTGATAAAAAGGAAGAGGAATACAACCAAAACCAATCCAGTTTCCATAACTTCAGCAAAGAGACACTGCAAAAGCAAGCAAAGCGTGGTTTTCTTTTATTGGAACGCTGTTCGTTGGTTGGGTTACAACAACTAGAGTTGGAGTATGTTAATTTGTTGGGCAGAAGTTTTGATTCTTATCAACAAAAAACAGAACTTTTAAACAACTTAAAGGAGCTTGTTGATGAACATTTCAGTGATACTGAAAAAATTATCAATACCCTTGAAAAGATCTTTGATGTCATTGGCGGTAGTGAATATACCCCTGTCGTCAGCGCTTTAAAAAGTTATCACAAAAGATTGACAGTTCTCTCAAACAGATAGAAACAGAGGCTAAAATAGCCACTAAACAGGTTCAAAATAGTGAAGTGATGTTCGGCCCCCCTGATATTGCTAATGATCATGAGTTAAACCTGAACTGACCTGATAGTGAAACAGATGCTATCTTAAGTTCAATGGAAAATGAATTGGAAGCTGCTTTACTAGCAAAACACCAAGAACCACCGTTAATTGTTACCCTACCCAGCTTAATAAAACCAACTGTTAGTCAACCTGAAGTTGAAGTTGTTACACCTACTAACAACACTAATTTTCAACCCCAAGTTGATCTCAAACCTACTGATTTGAAAAAACAACACAAGAAAAAACCACTTAACTTTATTACCCGTCCTGTTTTCAAAAGTAATTTGCCACCAAAACTAAGTAAGGATGACATAGTTCATTATGCGCACCAGTTACTTGAAAAAAATACCCATAATGAATAGTGATAGTGATCTAAAACTCCAAAAGGTGTGGATCGAGCGGCATGTTGATCAAGATGAACTTAGTTTAACAACTACTGCAGTTGAACTTAAAAAGAGTGATGAACAAAAACCTGTTGCCATTAAAAGTAGTGACTTTATTGGCCATGAAGAATTAATCTCTGTTCCAGTTTTACTAATCCCAACCCCTGTTGTTAAAGAGATTGACCAACCAGTAGTTATTACTCCAGTTAAAGCAAAACCAAAAGCAACTAAAAAGAAAACTCCTGTTAAATCAAAACCAACTAATAAATCAACTAAACAAACAAAACCTAAACAACCCAAACCCAAATCAAAACAAGTTCAAAAAACCAAAGCTAAACCAAATCTTAATCTGGTTTTTTTAGTGTTAACAACAGTAGTTTTAACTAAAAACTGGAAAGGAATAGGAAGAACTACCTGGGGTTAATCCTAGTTTAAACCGTGCGGTGTTAAAATAATTGCTTTTAGAGCGATAACTATTATTTTTATTCTCATTATCATCATCAGAACCATTGGCATTAGCTGTGGTACTTTGAAAAATGTCATTTACTGCATGTGCAACCCCAGTAAGTGCATTAACTATCATAATAAGTCCTGCACTAAAAGTTCAGAAAGATAATCCACCAACAACTTGCTTAGCTTGTGCTTTTTCTAATTTATACATATAACTCCTTTTGTCTATTAATCACATCTAAATCTAAAAGTGCCAAAGTTTTATAATTGATCAACTGTCATCATAGCTCAATAGGACAGAGTATCAGCTTGCGGAGCTGAGGGTTACAGGTTCGATTCCTGTTGGTGACGCCATTAACTTTATTTGCCTATCAGTTAAATAACTGGTAGGCTTTTTTATTGTTTTGTAGTTTATCAAGGGTTAATTTAAGTTGTAGTCATTTCATTTTGGACAAAAAGAAATTTTTATGCTAAGATAAAAGTGTTTAAAAGTGTCGCAAAGTGTGACAAAGTGGAAAAAATGCTGCTAGGTACCTTTAATCTTACCCTTGATAACAAGAACAGAATTAGCTTGCCAGCTAAGCTCCGTAGTTTCTTTGATAGCAGCATAGTTATTAACCGCGGCTTTGAAAACTGTTTGGAAATTAGAAAACCTGCAGACTTTGAGAGTTATTTTCAAACCTTTAATAACTTCCCTAACACCCAAAAAGACACAAGAACATTAAAACGCTTAATCTTTGCTAATGCTAATCTAGTTGAACTTGATAGTGCAAACAGAATCCTAATCCCTAATAACCTAATTAGTGATGCTAAGTTAGATAAAGAGATCGTGTTAATTGGTCAATTTGACCATCTTGAAGTTTGGGATAAAGTGCAATATGAACAATATCTAGCTAGTTCAGAATCACTAGAGACAGTAGCTGAAAGGATGAAAGATGCTAAATAACCAACAGATCCACCAGAGTGTACTGATCAATGAAGTGATCCATAACCTCAATATTAACCCTTGTGGTAACTATTTAGATCTAACTGCAGGGTTTGCAGGACACAGTCAAAAGATCTTAGAAAAACTAACAACAGGAACTTTAACAATTAATGATGTTGATAAAGAAAGTATTAATTTTTGCCAAAAGCTTTTTTTTAAAAACAACAACGTTGTTATTATTCACGATAACTTTGCTAACTTCCCAGTTCATCTTAAACAACTATCAATAACCAAGTTTGATGGGATCTTAATGGACCTTGGTGTATCAAGCCATCAACTCAACCAACCTAATCGCGGTTTTAGTTTTAAGAATGATGGACCGATTGACATGCGTATGGACCAATCCAATCAGAAAAATACCGCACTAACAGTTTTAAAAAACTTAACTGAACAAAAGTTAAGTCTAATCCTTAAAAAGTATGGTGATATTAAACACCCTAAACCAATTGCTATTGGATTGAAAAAAGCAGTTCAAACTGAAAAAAATCTTACCACAACTCAACTAGCAAAAGTGGTAAAAGAATGTGCTACTGGATTTGAAAAATACCAATCAAGAAACTATCTTGCCAAAGTTTTTCAAGCAATTAGGATCTATCTTAATGATGAGATTACTAATCTGAAAACTGCGTTAACTTTTATCCCTAATCTTTTAAAAAACAACAGCAGGTTTCTTGTGATTGTTTTTCACTCCATTGAAGAAAAAGTTGTAAGGAATTTCATTGCAAAACTAACCAGCTTTATCCAACCTGAAGCTCTACCCATTAAACTCACTCCTGCTTACCAGTTAATTACAAAAAAACCAATCCTACCTTCCCAAAAAGAACTTGAATTAAACCCGCGTTCGCGTAGTGCCAAACTCTTTGTTATCCAAAAAAACTAGTATGTACAAACCAAAAAATATTAACAGCGTATTAACCTTTTATAAGGATCAGATCCAACTGGTTGTTAGTGATGATCAAAACCAGTTCAACATCTTGTTTTACCAAACAATTGATAACGATGGCTTTTATTCAAAACAACAGTTGAAAAACAAACTAAGACTCAAGTTAGCATTAAACCAACTAGTTGATCAAGCTAACTATTTTCTTGGTTTTAAACTGGAAAAGGTAGTTGTTGTTCTCGCTGAACTGATTGATGATTTGAAGATCCATAATTTCAAGAGTGAGATCTTTTTTACTGGTTATGATTTTGATCATAAAGCGATGATTAAAAAAGAAAAACAACGCTTTTGTGAGCAAAATAACCAACTAACAGTTATGGATACAATGGTTTTAAACTACCATGATGTTATTAACAATAAGATCACCAAAAGCTTTGCATTTAACAAGAGCTATGTGGCTAATTTAGTGGCATATTCCTCTAAAAGTAACCTGATTGGTGAGTTGAAGTTCTTTTTAAAAAGAAACGTTAATCTTAAGGTTAAAAAAATTATTAGTCACCACTTGGCATTAGCCAACTCCTTAAGTAAGAAACAAAACAACATGTTTGTTTATTTAGGACAAAAAACTACTGAACTGATGCTATTTATGGACAATGCTTTAGTTGATGTTATTACCAACCAGTTTGGTAAAAACCACTTTATTGATATTCCAGCTAACCAGGAAAACAAACCACTGCTTGAGTTTTTAGTTGATAACACCACTAAGATTGGTGATTGTTATTCGCTTGGTATGACCTATACAGATGGTGATAGTTACAAAGAGATTAAGGCTTTGACTATTGGTGATTTAATGCAAACAGTTAGTGACAAGATCAAAACCTTAATTGATTTTATTAACAGTGGTTCTCTAACTTTTTTCAACAAGTTTAAAACCTTACCTAAGCTATTGTATTTTTATACAAGATCAAAACAAATTACCAACCTTTTTCAAGCTAATGTTGCGCTTATCAATCCCCAGTTTAAAACTGTTGATATTTATAAGAACAAGATCCAGTTTATTAGTGAAAACTACCTGTTAAGCTGTGAAGCGATTAGCCTGCAGATTACCAATAGAATCAAAAACCAAATTAGTTTTGATTTCACAAATGCTGATAATATTCAAAAACCTAAACCAAAAAAACACTTCATGATCTTATCAAAACACCTAACAAAGTTTGTCCAACGCTTGGTTAAATAACTATGGATGAAAATGAAACTCAATTCAACAAGTTAAACCAAGTTAAAAACAAGCTGAAAATTGGTGTTTTTGGGATTGGAGGTGCTGGTAATAACATTGTTGATGCATCACTTTATCACTATCCTAATTTAGCAAGTGAAAACATCCACTTTTATGCTATAAATTCAGATTTACAGCACCTTGCATTTAAAACGAATGTTAAAAATAAACTCTTAATTCAAGACCATACTAACAAGGGCTTTGGAGCGGGGGGTGATCCAGCTAAAGGAGCTAGTTTAGCAATAAGCTTTCAAGAACAGTTTAATACACTTACAGATGGGTATGATTTTTGTATCTTAGTTGCTGGATTTGGTAAGGGTACTGGTACAGGTGCTACCCCAGTTTTTAGCAAGATCTTAAAAACTAAGAAGATCTTAAATGTTGCTATTGTTACCTATCCATCTTTAAACGAGGGATTAACAGTGAGAAACAAAGCAACTAAGGGACTTGAAATTCTCAACAAAGCAACTGATAGTTACATGCTATTTTGTAATGAAAAATGTACAAATGGTATCTACCAACTAGCAAACACAGAGATAGTCAATGCCATTAAAAACCTAATAGAACTAATTACTATTCCTTTGCAGCAAAACATTGATTTTGAAGATGTACGTGCCTTTTTTCAAACCAAAAAAACTAACCAAGATCAACAGCTTTTTACTGTTACTCACCCCTTTAGTTTTAGCTTTGATAGTAAAGATAGTATAGAACAGTTTGCCAAACAGTTTAAGAACTTTGAAAAAGTTAGTTATTTTGACCACTCTATAGTAGGAGCTAAAAAAGTGTTATTGAAAGCTAACATTAACCAAAAGATAGTCAAGCTTAATTTCAAGCAGATTCAAGATATTATCTGAACTAAAATTGACAACTACCAACTTGAGATTAGGTTAGGGGTTGATTTTGTGACAACCATCCCTAATATCCAAATTTTTATCCTCAGTGAACACAAAAATCCAGTTTCACTTCCCATTGATAATAAACCAACGGAAAACAACCAAAATAAGTTGAAACTTTTAGATGAACTGAAAGAACTTGGCATGAAATATGTTAAGCACCAAAACCAAATCTACTAATTAATTTAATTTATCGTTTAGAATTGCTATCTTAAGCAATAGTTTATGGGTCAAATCAATCGGAAGTTTAGCGAAAAGCAGTTCTTACTTTTTGTTGTTAACTATATTGCTGGATTTGGCTTTATTGCTACTGCTATCTCACTGTTTCGCTTAGGACCTTTTTCTTGGTTAATCTTTCTGCTTGTTAGCTTAGTGAGTTTAATTGTTACCTTATCATTCGCACGGCTTTCATCAATAGATAGTCAAAACTATGGTGGGCCTTATCTTTGGGCTAAGAAAGCGGTTGATAAAGAGAAGATAGCAGGGAGAATGTTTAGCTTTTTTACTGGGTGAAATAACTTTATCATTGGTCCCCTTTCAGCAGCAACTGCACCACTTTTTATCCTCAATTCCTTTAGTGGTATTGATGGGATTAGAGGTAACTTAGTTAACACTTGAATCCTAATTGCAATAGGTTTTTCTTTTTATGTATTACTAGCATTTATCTCAACCAAAGGAACCTCACTAAACAAGAAACTAATAGCACTATTTGCTTCAGTGAAGTGGATTGTGATCCTCTCAGCACTAATAGTAGCAATCTATGTTATTGCTAGAGATGGTAATGGTTATAGTAAAAACAATAACTTAGAAAGTGGTTTTTTTGGGAGAAGAGAGATTAGTTTTGCACAGATAGCAACGGTATTTATTACCTTCTTTTATTCTTATGCAGGGGTTGAAGATATCTCAGTGATGACTCCTGATGTTAAAACTAATAACTTTAGAAAGATATTAATTGTCTCTTTTATAGCAGTTTTCCTCTTTTATTTCATTGGGATTATTATTCTAAATGGTTTGCAAAACATTGCTCAAAGAGGTGGGGAAGCCAATTCAATTGGTAATGTAGCGGATATCTTTAAAAAAGCTGCTGGGCTTGGGACTTTAATCTTTTATGGAGTTGGAGCATTGTTTAACAATGTCTCAACCAGACTTTCAACTATTATTGCCAACTCCAGAAAGATTCTTCCGCTTGCTTATGATAACTATTTACCTAGTTTCTTTTACAAGCAAAACAAAAAAGGTGAGTTTCAGAATGCAATTTGGTTTACCTTTGGTACTACTTTAATTGCAATGACTTTGCTTGTCTTTATCCCTTTAGTTGCTTCTAACTTTGATTTTGATAATGCTACTGAGTATGCAGCATCTGTTGGCTCAGCTGCAACTTTGCTACAATATATTTTTGTTTTTTTTATAATCTTTAAGTTTATCTATAAAAAAGAACCACTCTACCAGAAAAAATGGGTTAAAACAACTGAAGAATTATTGTTTTGTTTAGGAACAATTGTCATTGTTTTAATGTTGTTGGTTTATCTGTTTCCTGTTATAGATGGATTTTCAAAATGGGAAACTAAACACACATTAACAATTGTGTTATATGGGGTTTTAAGCCTGATAGGATTGGTACTTTTTTTGCTCCAAGAATACAAACATAAAAACAAGCAAAATGCAAACAAACAAACAACCCAAACAACAGTTTAGTGAAAAGCAATTCATTGCTTTTGTCTTTAACTATATTGCTGGATTTGGCTTTATATCAGTGGTGATGACCATGTTTGATGTTGGGCCATTTTCCTATCTTGTTTTAGGGTTAACTTCGTTTGCTATTTTAGGAGTTGTGCTTTCTTTTTCTCGCCTTTCAGTTCTCTGTGGTAATAGTGCTTATGGAGGGAGTTATTTAATTGCTAAAAAAGCAGTTGGTACTAACAGTAAAACAAAAAGGTTTTTTGTTTTTTTAAGTGGGTGGAATGTATCGTTAACAGGATCTTTTAACGGTGTTGTTATTCCAGCAGTATTAATCTTTTCCTTTGCAGATATTCCAGTAGTTAAAGCGAATAATAACATCATTATTGGTCTTTTAGTAGGTGGATTTTTGTTGTTTGGCTTACTTACTTTTATCTCGTTATTTGGTTTAAAAATTAACAAGAAAGCAATCTTTTATTTTGCTGTTATTAAGTGGATAGTAGTAATAGGTGGGTTTATCTTAGGGATCTATTTAATTGGTACTACCAATGGTAAAGGGTTTGTTGAAAACAATTTAATTGGGACTAGGGAAAACATTGATTTTTTCAAGATTATCTTTATTAGTCTGGCTTTAACTATTGCTTTTGCAGGGACAGAGGATTTAGCTTCGATTACTCCTGATGTCAAGTCAAATAACTTAAGAAAGTGTTTTTTAATTGCCTTTGGGTGTGTTGTGTTACTTTACCTAGTTGGGTTTGTTATTATCAGTGGACTTGATGGGATTAGAGGTTATGGATTAGCATTAGGTAATAAAGATCCCAAGGCAATTAATAACTATGGATCTATCTACCGTTTGGTAGGAGGAGTTCCTTTACTTGTTATCTATGGACTTGGGTTACTTGTCAATTCCTTAGCATCACGCTTATCAATGACAATTACAACAGCTAGAAAATATGTAGCTTTAGCTCAAGATGGGTTTTTACCCTCTTTTTTAGCCAAAACTAATAAACATAATGAGTATCACCATGCAGTTTTAATTAGTAATCTAATGACTTTATTAGTGATGCTAATTATGGTAATAATCCCCTTTTTACCAGACCATAACAACAATAACAATAGTTTGTTTAATGCTATTGAACAGTTGGTTACAGTTACCATTGAAATGGCTGCAGCCATTTCTTTGATCCAATACTTTATTACCTTTATCTTCTTTTTTATGATCTTCGCTAAAAAGGAAAACCAGAAGTTAATTCCCTTGTGAGAAAAGGTTAGTTATGTAATCAGCTTTGCTTTGGTAAGTGTGTTGTTGTTTGTACCACTTTTCCCTTTTAATCAGTGAACAGTGTTTAACACCTTTAAGATAGTTGTTCTAATTTGTTTTTATCTACTTGGTGTTGGTTTTTTTGGTTATGCTGAATGGAAAAATAAAAACAAATACCAATTAATGAATAGTAATAGCTAATCTACAGTTCATTAAGCGAAATTGCTTTTGAATCGGCAACAACAAACCCTTCTCCAACTCCCACACTGCTACCCTTTCTGTTAGTTCAAATACCCCACTCCCCACTTTTTCTAACATCAATGTTGGGGTTAAATCAATGATCACTCAACACTTAAACAAAGAAAACACCCGGTGGGTGTTTACCCCTAACTCTTCACCAGACATTTGAACTGGGGCTGGGTATCGCAAGGGTTCATCAAACACCAACGGCATCTCCTTGACAAGTGTGTTGCCTAGTAGTAGTAGTAGTAGTCCTTCGTTTAATCCCTCCTCCATGGAAAACAAAGTCACCCCAGCTGGTGGCTCCTCCAAAAAAACTACATACACCCATTTACCCAACAGTATCAGTCCCACCAGTGACTGTTAAAATGTTTATTAAAAAAACAAAATAAAAAGCGGTTTTATACAATGTATAACCTGTCTAAAAGACAATTTCATGAAACAGTATTTAGATTTAGCTAGTTATGTTTTAGCAAATGGTAAAAAAAGAAAAAACCGTACAGATACAGATACTTTAAGTGTCTTTGGTTACCAGATGAAATTTGACCTTACTAATAGTTTTCCTTTATTGACAACTAAAAAGGTTAATTGGAAGGCAATTGTCCATGAATTGTTGTGATTTATTAAGGGTGATACCAACATTAAGTACTTAGTTGATAATGGGGTGAACATCTGAAATGAATGACCATATGAAAACTTTAAAAAATCACCAAGTTTTCAAAACGAAACACTCCAAGAATTTATCTTAAAGGTTAAAACTGATAATGAGTTTGCTAAACAATTTGCTGATTTGGGTCCTGTTTATGGCAAGCAATGACGTAATTTTAATGGTGTTGATCAACTCAAAAAAGTCATCCAAGAGATTAAAGAAAATCCCAACTCAAGAAGGCTAATTGTCTCAAGCTGAAACCCTAGTGAATTGGAAAAAATGGCATTGGCTCCTTGTCATTCACTCTTTCAGTTCTATGTTGAAGAAGATAAACTAAGCTTACAGCTTTACCAGCGCAGCGGTGATATCTTTCTTGGTGTCCCATTTAACATTGCATCTTACGCCTTACTTGTGTATTTAGTTGCCCATGAAACTAAGTTAAAACCTGGTTATTTTATCCATACACTAGGAGATGCACATATCTATGAAAACCACATTGAACAAATTAAATTACAACTAACAAGAACAACCCTAGACCCCCCTCAAGTGGTTTTGAAAAGTGATAAATCAATCTTTGCTTATAGTTTTGATGATATTGAGTTAGTTGGTTATAATTACCATCCATTTATCTATGGGAGGGTTGCAGTTTAATGCTAATTGCTATCTGAGCGATGACACAAGAAGGACTAATAGGTAATAACAACACTTTACCTTGGATGATTAAACAAGAGCTAGCTCACTTTAAAAAAACTACGTTATTTCAAGCTTTGTTAATGGGGAGAAAAACTTACGAATCACTCCCCAAGGTATTTGAAAAAAGAACAATATTTCTCCTTTCAAAAGATCAAAACTACCGTTTTGAAGAAAAGGGAAGTGAAGTGAAAGTTATTAATGATTTTTGACCACTAATTAAAAGTTACCAAGCAAATAAAGAAAAGGATTTGTTTATTTGTGGTGGAAAAAGTGTGTATGAACAGACCATTAATGAATGTGATCAGTTAATTGTTTCAATCATTAAAAAGAAGTATAAGGGTGATCAGTTTTTGAAGGTTGATCTCAGTAAATTTGTACTTAATGAAGTTGTAGAGTTTGAGGAATTTAATGTTAATTATTATAGAAAGAAACAACAATAAGAGATATGGCAGCTAACAATAAAAAGTACTTTTTAGAATCATTTTCCCCACTTGGGTATGTAAAGAATAATTTTCAGGGCAACTTACGTTCTGTAAACTGGAATTTGGTCGATGATGAGAAGGATTTGGAAGTGTGAAACAGGATTGTTCAGAACTTTTGGTTACCTGAAAAGATCCCTGTATCCAATGACATCCCCTCATGAAAGAAACTCTCAAAGGATTGACAGGATCTGATCACTAAGACCTTTACTGGTTTAACACTACTTGATACTATCCAAGCTACCATTGGTGACATCTGTCAAATTGATCATGCTCTAACTGATCATGAGCAGGTTATTTATGCAAACTTTGCTTTTATGGTAGGGGTACATGCCCGTTCCTATGGAACGATCTTCTCAACTTTATGTACATCAGAACAGATTAACGCTGCTCATGAGTGGGTTGTAAACACTGAAAGTCTCCAGAAAAGAGCAAAGGCATTAATCCCTTACTATACGGGAAATGACCCGTTAAAATCAAAGGTAGCAGCAGCTTTAATGCCTGGGTTTTTACTGTATGGTGGGTTTTATTTGCCTTTTTACTTGTCATCAAGAAAACAACTACCAAATACATCTGATATTATCCGCTTAATCCTTCGTGATAAAGTGATCCATAACTATTACAGTGGTTATAAATACCAACGTAAACTAGAAAAACTCCCTTTAGCAAAACAAAAGGAGATGAAAGCATTTGTTTTTGAACTAATGTATCGGTTAATTGAACTTGAAAAGAACTATTTAAAAGAGCTTTATGAAGGGTTTGGAATTGTTGATGATGCCATTAAGTTCAGTGTTTACAATGCTGGTAAGTTTTTACAGAACTTAGGTTATGACTCCCCGTTTACTGCAGCAGAAACCAGGATTAAACCAGAGATTTTTGCCCAACTATCAGCACGTGCTGATGAAAACCATGACTTTTTCTCAGGAAACGGTTCGTCGTATGTGATGGGAGTTAGTGAAGAGACAAATGATGATGATTGGAACTTTTAAGTTATGCATAAAGATATCAAACTAGTTAAGGAAACTGAAATTAGAAAACCAATTGGTTCTCCTTTTATTGTCTATTTTTCATCTATCTCCAACAACACCCACCGTTTTATTGAAAAACTAGGTTTTCAACACAAAAGAATCCCAGTTGATATAACCCAAAGCATTACTATAAGTAATGAGTATGTTTTAATCTGTCCAACTTACAGTGGTGGGGGTAACCAGGTTGAAGGAGCGGTACCCAAGCAAGTTATCCAGTTTTTAAATAACAAGCATAACAGGGAGTTATGCAGAGGAGTTATTGCATCTGGTAACACTAATTTTGGAGATACTTTTTGTCTTGCAGGAACTGTTATTTCCAAAAAACTAAACGTCCCTTTGTTATATCAGTTTGAACTTTTGGGAACAAAAAATGATGTAGAACAAACTCAAAAAATAATTGCCAATTTCTTTCAAAACAGCAACTAGTATTTATAGTTATCCACTATGACATCCAAAGAAAAAATCCCTACTTTTAATACTGAAGAAGATGTTGAAAGTTACATTTCTTTTAATGCCCAAGCCAAAATCTATGATGATTTTGCAATCGATTTACAAGCAGTTGAAAGCTATATTCAAGAGCATGTAAAACCCAAAACCAAGGTCTTTCATTCCACCAAAGAACGCCTTGATTTTCTGATTAAGAACGATTATTATGATGAGAAGATCATCAACATGTACAGTTTTGAACAGTTTGAAGAGATCACCCATAAAGCATATTCATACCGCTTTCGTTATGCTAACTTCATGGGAGCATTTAAGTTCTATAATGCCTATGCTTTAAAGACATTTGATGGTAAGTACTACTTGGAAAACTATGAGGATAGGGTGGTGATGAATGTATTGATGTTAGCTAATGGCAACTTCAATAAGGCATTAAAACTCTTAAAACAGATTATCCTTAACCGTTTTCAACCAGCAACCCCTACCTTTCTTAATGCTGGTAGAAAGAAACGTGGTGAATTTGTTTCATGTTACCTGTTAAGGATTGAAGATAACATGGAATCAATAGGTAGAGCGATAACAACTACACTACAACTATCAAAACGTGATGGGGGAGTAGCACTTTTGCTTTCCAACTTACGTGAAGCGGGAGCGCCCATCAAAAAGATAGAAAACCAATCATCAGGGATTATCCCAATTATGAAATTGTTAGAGGACTCTTTTTCCTATTCCAACCAACTTGGACAAAGACAAGGAGCGGGAGCGGTGTATCTCCATTGTCACCATCCTGATGTTATGCAGTTTTTAGATACTAAAAGGGAAAATGCTGATGAGAAGATCAGAATTAAATCACTCTCCTTAGGACTTGTGATTCCAGATATCACCTTCCAATTAGCAAAAAATAACGAGATGATGGCACTTTTCAGTCCATATGATATCTATCAGGAGTATGGTAAGGCTTTATCTGATATCTCAGTAACTGAGATGTATTATGAATTGCTTGAAAACCAACGCATTAAAAAGACCTTTATTAGTGCTAGAAAGTTCTTTCAAACAATTGCTGAACTCCACTTTGAAAGTGGTTATCCCTACATCTTGTTTGATGATACAGTTAACAGGAGAAATGCCCACAAAAACAGGATAGTAATGTCTAACCTTTGCAGTGAAATTGTCCAACCATCTTTACCTTCTGAATTCTATTCAGACCTTACTTTTAAAAAGGTAGGTAGTGATATTAGCTGTAACTTGGGGAGTTTAAATATTGCTAGAGCAATGGAAAGTGGTAGTGAGTTAGCTGAATTGATTCAACTAGCAATTGAATCACTGGATTTAGTGTCAAGGATCAGTAGTTTAGAAACCGCTCCTTCCATTAAAAAAGGTAATTCAGAAAACCATGCGTTGGGATTAGGAGCGATGAACTTACATGGATTTTTAGCAACAAATGCTATCTATTATGATTCAAAGGAAGCGGTTGATTTTACTAACATCTTTTTTTATACAGTAGCATACCATGCGTTTAGTGCTTCCAATAAATTAGCATTGGAACTAGGTAAATTTAAAGACTTTGAAAATACTAAATTTGCTGATGGTAGTTACTTTGATAAGTACACTAAGGTAGCTAGTGACTTTTGAACATGTAAAACAGAAAAAGTTCAAGCCCTTTTTGATAAATACCAAGTAAAAATTCCAACTCAGGAAAATTGGAAGCAATTGGTAGCAAGTATTCAAAAAGATGGACTTGCAAACTCCCATTTAATGGCTATTGCCCCAACTGGATCTATCTCATATCTCTCTTCATGTACCCCTTCACTTCAACCAGTAGTATCTCCTGTTGAAGTGAGAAAAGAAGGGAAGTTAGGACGGATTTATGTCCCTGCTTATAAGCTTGATAATGATAACTATCAGTACTTTAAAGATGGTGCTTATGAACTGGGCTTTGAACCTATTATTAACATAGTAGCAGCAGCCCAACAACATGTTGATCAAGCAATCTCTTTAACCTTGTTTATGACTGATAAAGCTACCACCAGAGATCTCAATAAAGCTTATATTTATGCTTTTAAAAAGGGTTGTAGTTCTATCTATTATGTCAGAGTAAGACAAGATGTTTTAAAAGATAGTGAAGATCACACTATTAAAATCAAGGATTGTGAGGTTTGTTCTATCTAACTATTAAAGCAGTTAGAATTTGTTAGAATTACTTGTTTTAAAACTATCTTAATCCCTAATATATAAATTAGAAAGGCAACGGTTTTGAGAAGATGCATGCTATTGTGGTTTGTGGTGCTAAGCAGTATTTAGTCCATGAAAACGAGTCTATTTTTGTTGAAAAATTAGCTGGTAAAGTTGGTCAGGAGATCCAACTTGATAAGGTATTGATGCTTGATGAAAAGATAGGCAAACCTTACCTTGAAAAAGCTAAGGTTGTTTGTGTGATTGAAAAACACGGTTTAAAATCGAAAATTAAACTAATTAAACACATCTCCCAAAAACACCACCTCAAGCGTTATGGCCACCGTCAACCCTACACCAAACTAAAAGTGGTACGCTTTATCCATGATTAAGATTAATATCTCCCAAAACTTTCTAGTTGCAAAAGGTCATGCTTTGTTTGCTGAGAAGGGTAAGGACATAGTTTGTGCTGCAATTAGTGGAATTATCTTTGGGGGGGTGGCTTGGTTTGAACCTGATAAGATTGAATTTACTGAAAATAAATTAGTACCTAGTATAGCACTGAAACTCATTGACCCAACCCCTAATGTAGCAGTTGCTTTTAGTGTTATTACAGTACAATTAAAAGCAATAGCCAATTCCTATCCTAATCACATAGTTATCAATGAAGAGAGTTATGAGTAAAAACAGTTACTGCTACCAAATTAACTTACAGTTTTTCGCTTCCAAAAAAGGGGTTGGTTCCACTAAGAATGGACGTGATTCACATTCTAAGCGCTTGGGCGCTAAGAAGGCAGATGGTCAGATGATTAGAGTTGGTCAGATTATCTATAGACAAAGGGGAACTAAGATCTTTGCAGGACAAAATGTTGCAATGGGTAGTGATAACACCCTCTTTGCTTTGAGCGATGGCATTGTCCGCTTTCAAAAGTTTGGTAGTAAACAAAGCAAAACCCGGGTTAACATCATCAAACACCAACTAAATGCCTAAGCTACTGGGTAGCTTTATTAGCTTTAAAGCCCCCAATTACTTTGTTCAAAGTGCTCAGGATGCAATTGCTATTGATGCAACTGCTCTAATGGTATTTTTAGGTCCACCCCATTCTGCTTACCGTGTTCCTTTTAACAAGATGCAGTTTAGTTTGGGCTATGAGTTGTTAAAAACAAAGAATATTAATAGTAATGGTTTGGTTGTTCATGCTCCATATATCATTAACTGTGCATCAAAAGACCCACTAAAACAGCAGAATGCTATCAGTGTTTTAACCAATGAGATTCAGCTTTGTAACTTGGCTGGTGCTCACTATTTAGTTTTGCATCCAGGTTCTGCAGTAGCCCAAACAACCAACGAAGCATTAGATAACCTGGTTAAAGTACTCAATCAAGTTATCAATAAAACCAAAACAACAGTTATTTGTCTTGAAACAATGGCTGGTAAGGGTAATGAGATAGGCAGAGACTTAACTGAGTTGAAATATGTTATTGACAGGATCGTTGATAAAGATAGGATCGGGGTTTGTCTTGATACCTGTCACTTCCATGATAGTGGGATTGACTTTAGTGATCTAACTGGTGTTTTTAACACCATTACAACTAAACTTGGTTTTGAGTTTCTCAAAGTAATCCATTTAAATGAATCTAAAAATAATTGTGGTTCTAAAAAAGATAGGCATGCTAATATCAATGCTGGGATGATTGGTTTTGAGAACTTAATGAAGTTCATTAGTCACCCCCAAATTAAGGATTTACCTATTATCTTAGAAACCCCTTCAACTAGTTTAAACTACCCAACTATTTACCGTGAAGAGATTAGTCAAATCCGCAGCTGATTTAAAACTTACCAACCAGATGCTAACTAGTTATGTGAAGGTATTAGAACAAAACAATCTGCGCTTAACAAAACCGCGGATTGCTTTATTAAAGTGTTTAATTGAACACCAAGATTGGCATAATCTCTCCCAAATTAAAACCCACCTTGATTTAGCCAATCAACCCTCAACACTCGCTTCTATCTACAACAACTTAAGAATCCTAGCTAAACTTAAACTGATTAACATCTTTGTAGATCCAGAGCGGTTTGAAACTTACTATTGCCTGCGCCATGCAGAGCATAACCATATCTATCTTTTTGATGAAGTTAAACAGCAGTTTTTTACCTTACCTTTAACAGATGGCCAAATTAAAACATTGCTTGAAACCCAAAACCATACCAGTAAGGTAAAGCTTAATGATTTTTATATTGTTGCTAGGGGTGAGATAAACAATGATTAACAAACCAAACCAATTTTTAAACCACCTTGATGGCTTAAAGCAACATTTTTCTGATTATGATTCACTACAAAAATCCTTTAAGAAATATCTATCAGAAAATCAAACTGAACTTAACAATTTCTTTTTTAACCAGTTTGAAAAGATCATTGTTCTGGTCAAAAAAAAGGAGTTTAAAACTGCTCAAGAGAGGTGTGAAGAGGAGTTAGCTACCCCTTATTTTTCAAAGCCATTGGTTGGTTTTTTCCAATCACTATTACAACTAATTAACCATGATCTCATCGAACAGAAAAACCAACAGTTAGCTAACATGAGTTGTGAAAAAATTGTGGAGATGGTGTTAAGTGATTACCCCAATAAACTTAACTTAATCCACTATTTGTTAGCAAAAGAAGCGAGTTTTGTAAACCCTAACCTTTTACAGCGGATGACTTTTGTGTTAACTGATCTTGAACTGTTAGAGTTAAAGCGTTTCTCTTTTTTTAAAGCCCTTAACCAGATACCTGCTTTTAAAAACCACAAAGTAACATACTTTAACAGCAAACTCAAACAGAAGTTTGTAATAACATTAGGTGAATTTGCTTTCCCCCAAACTGATAAAACCAAACAGTTTTTCCAACAACTAATTAAAAAAGTAAGTCAACTGTTTTTAAAAGAACCTGTTAGTTGTGAATTTGCTTATGAAATTATTGATGCATTACTCGTCAGTTTTTTTCCACTCCATCCTAATTTAGAAGTAAACCACTTAGCTAAAAAGATCCACCAGTATGTTAGTAAGATTGTCATTAATGAAGTTGTTGATCTGAAAGATCCAACCACTAAACTAATTGTTGATACACTTTATGAACAGTTAGATAGAGCAATTGGTGAGGAAAATTAAAATTAAGTTAGCACTAGTAGATACAAAAGATGAAGTTATACAAAGTTCTTAACAGTAAAACAACTGATAAAAGTCTTTGTTTGGAAGTTGAGATTGATCCAAATTACTGACAAGCTACCCAAAAAAAACTAGTAGGTGAAATGGCTAAATCGATAAAAATTAAGGGTTTTCGTCCCGGTAAAATCCCCCCTAATTTAGCCAGTCAGTCGATTAATAAAGCTGAATTAATGCAAAAAAGTGCCCAAAACGTCATGAACAGTATTTATGAATCAGTTCAACAAGAAGAGATCGTTGCTAGTAATGATAATGTCATTGATGATTATCCTACCATTGATTTCAAAACGATCACTGAACAAAACTGTGTACTTTTGTTTTACTTTGATCTGATCCCTAACTTTCAACTCCCTGATTACAAAAAGATAAAAGATTTAACACCACTTACCAAGTTAACTGAAGCTGAATTTAACAACGAAATTGAAAAGCTGGCAAAAACTAAAAGCACAATGGTAGATGTTAGTGATAAAAAACTAGCTAATGGTGATATTGCTATCATTGATTTCACTGGGATAGTTGATAACAAAAAACTAGCATCAGCTTCAGCACAAAACTATGAATTGACAATTGGTTCAAATAGCTTTATTAAGGGTTTTGAAACCGGGTTAATAGCAATGAAAGTTAACCAGAAAAAAACTTTAGCACTAACTTTTCCTAGTGATTATCATGTTAAGGAGTTGCAATCAAAACCAGTTACATTTGAAGTAGTTTTAAAAGCAATTAAAAAACTGGAATTCACCCCAATGGATGAAACTAATTTCAAATCCTTTCTCCCTGAACAATTCCAAAGCTTTACTTCTCTAAAGGCATTTAAGAGTTATTTTCATAAGCTAATGGAAAACAAAAAACAAGAGACAATTCTCCAGGAGAATAACCAAAAAATTCGTCAGTTCTTACTTACTAATACCAAACTTCCTTTTCTTCCAGAAGCGTTAATTAAACTAGAAGCTAACCGCTTGTTAAAGCTCCAGCAAAGCCAAGCTGAACAATATAAAATCCCCTTTGAAAAACTCTTAAGTGCTTCTAATATCACCCTAACAGAGTTACAAGATCGCAACATAAAAGAAGCTAAGGAAAATGTTACCTTTGCTTTGGTAATGAAAAAGATAGCTGACATTGAAAAGATTAAGGTTGATAATAACAAGATTAAAGCTGAAATTGAAAATGTTATTGCTGTTGAATATCCCTTTGCTAGTGATGAAATGAAAAAACAACTCTTTTTTAATATGGAACAACAAAAGGAGTTTGTGGAATCAATTATCATCAACAGATTAACAACAGCTAAAATCGTTAGCTATTCAACTCATTAGCACTCAAAGCTTGTGAGTGCTAAGAAATGTGTTAAAATTTATTGAAATTCCCTAATTAACTTTTAAATATGCCCGTTACGAAGAAAAGTCAGATCTTAGTAGTTAGAGGTCAAGTCATTTTTCCTTTTGTTCCCTTTAGTTTGGATGTTGGCAGGCCCCGTTCGCGTAAGATCATCAAAGCGCTTAAAACTCTGAAAACCAAACGTTTGGTTTTAGTAACCCAAAAGTTTACTGGTGAACAAAACCCTGAGTTTAATGACATCTATCATGTCGGTACACTCTGTGAGATTGATGAGATAGTTGATGTTCCAGGGGTTGATAGTAAAACAGTAGACTACCGTATTAAAGGCAGAGGTTTACAACGGGTTTTAATTGAAAAATTCTCAGATGCAGATATTAATGAAGTTAGTTACCAATTACTTAACTCCACAGTTAAAGATGAAGCTAATGTTGACAGGTTCTTACAGCGAATCTTTCCTGAAAAAGAAGAAATTGAACAGTTAATGGAAGGAGCTGAGAAGTTTTTAGAACTTGAAAACATCAGCAAAACAGTTAATGTTCCTAAGGGTTTAAAGCAACTTGATATTATCACCTTTAAACTGGCTAATCTTGTCCCTAACACTGAAAGTATTAAACAAGCTATCTTAGAGGAAAATGAGATAGCAAACCGATTGGAAAAGATTATCCAAGCAGGGATTGAAGATTTACAGAAGATCCAAGATTATGGTAGATCTAAAAACAAGGAAACTGAGTTTGATAAACTTGACAGTAAAATTACCCGCAAAATTAACGAACAACTCTCAAGACAACAACGTGATTTCTATCTTCGTGAAAAGCTAAGAATTATCCGTGAAGAGATAGGGATTAGTTCCAAAAAAGAGGATGAAGTTGCTAGTATTAGAAAGAAACTGGATGAAAACCCTTACCCTGAAGCCATTAAAAAACGGATTTTAAGTGAACTTGAACACTATGAAAACTCTTCCTCCTCTTCCCAAGAATCAACCTTAACCAAAACTTACATTGATACGCTTTTAAACCTGCCTTGATGACAAAAGAGCAAAGATAACAGTGATGTTAAAAACTTAATTAAGACGTTAGATAAAAACCACACTGGTTTAGATAAGGTTAAAGAAAGGATTGTTGAGTATTTAGCAGTACAACTAAGAACCCAAAAAAACAAAGGTCCTATTATGTGTTTAGTAGGTCCTCCTGGGGTTGGTAAATCAAGTCTAGCTAAGTCTATTGCAGAAGCATTAGATAAGAAGTTTGTCAAGATCTCATTAGGGGGAGTACATGATGAATCGGAAATCAGAGGTCACCGTAAAACTTACTTAGGTTCTATGCCAGGAAGGATTTTGAAAGGGATGACCCGTGCTAAGGTAATTAATCCCCTCTTTTTACTTGATGAAATTGATAAGATGACCTCCTCCAACCAAGGTTATCCTTCAGGTGCTTTACTTGAAGTATTAGATCCAGAGTTAAATAATAAGTTTAGTGATAACTATGTTGAAGAAGATTATGATCTTTCTAAAGTAATGTTTATCGCTACTGCAAACTACATAGAAGATATCCCTGAAGCTTTACTTGATAGGATGGAGATAATTGAACTCACTTCCTATACAGAACAAGAGAAGATTGAGATAGCAAAAAACCACTTAATTAAGCGTTGCCTTGAGGATGCTGATCTTAACAGTGAAGAATTGAAGTTCACTGATGAAGCAATCAGCTACATCATTAAGTTTTACACAAGAGAAGCGGGGGTTAGACAATTAGAACGATTAATCCAACAAGTTGTAAGAAAGTACATAGTAGCAATGCAAAAAGATGGCATCAAACAAGAAACGATTGATGTAAACGCTGTTAAAAAATACCTTAAGAAGGAGATCTTTGATCACACTATGCGTGATGAAGTGTCTCTACCTGGAATTGTCAACGGGATGGCATACACCCCAACTGGAGGGGACTTACTTCCCATAGAAGTTACCCATGTTGCTGGTAAAGGAGAGTTGATCTTAACTGGTAATTTAAAGCAAACAATGCGAGAAAGCGCTAATGTTGCTTTAGGCTATGTAAAAGCTAATGCAGAGCGTTTTAACATTAATCCTAGTTTGTTTAAAAAGATTGATATTAACATCCATGTTCCAGGTGGGGGAATTCCTAAGGATGGACCTAGTGCTGGTGCTGCTTTGGTAACTGCAATCATCTCATCATTAACTGGTAAGAAAGTAGATCCTACAGTGGCTATGACAGGAGAGATCACTTTAAGAGGCAAAGTGTTGGTTATTGGTGGGGTGAAAGAAAAAACTATCTCAGCTTACCGCGGTGGGGTTACAACTATCTTTATGCCTGAGAAAAACGAGCGCTATTTAGATGAAGTACCCAAAGAGATAGTAGATAAACTTAACATTATCTTTGTTAAGGAATACAGTGATATCTACAACAAGCTTTTCAGTTAGTCTTTAACTAGGTTTTGGACATATTTTAAGGTAATTAAATAGGCCTGTTTTAAGTTGCTTTTAGCTAGTTTTACTAGTTGATCAATCTCAATTCCCCCACTCCACTGTTCCTGTTTACGGTTAGGTTCTAGTTTATCTGCTAAATAAACAATCATATCTAGCTTGCTAACTTGTTTTGGTGGGATGGTGTGGTTTTTGATGGCATTAATAATCATTTTATCTTTAACACCAAATCAGTTTTTTAAGATATAAGCCCCCACATAGCTATGTAATACTTTTCAACTTGGGTAGTTAGTTATCTTCAGTTCACTAGTTGCAATGTTAACTAACTGATCAACTGGTAGTTGTTTGGCCAAGTCATGATAAGCCCCAGCAACAAATGCTCTTTTAGCATCTAGTTTGTTTGCAATTGCTAGTTGTTTTGCTAGTTTACCAACCCTTAAACAGTGTTGAAACCTCTTATCATCAACCATTGCTTTTAAAGTGGGAATTAGATAGAGGTGATTGGTATTGATGTAATTTAAAACTGCCAGTGGTATCAGTTTTTTTCTAGGTTGATTTAAAAGTTTACTTGAAGCGATTTCAAGTGGGCATTTAGCCAAGTATTTAACATTAAATTGGTTTGCTATTTTTTTATTAAAAGGATAAGGTTTTCGTTCATAACAAACAAACGTGCACAAATCTTTTAACTGTTGGATGTGATCTCACTTCTCAAGTTCATTTAATTTATCACTCCCTATTAAAAAATAGATTTCACTAGTTGGATAACAACTTTTAAAGTGGTTAACAGTATTAATTGAAAAAGCGTTTTTAGTTTTAATATCAAAATTGGAAACTAAAGCATTGTTGACTGATTTAATTGCTAGCTTTAACATAGCAATCCTATCCTTATTACTAGCATGAAAATTATTTTTAAAAATACCATTGTAAGTTGGTACAAAAAAAAGCTTTTGCGCTTTTATTTTTTTAATTGCATGTTTAGCAATGTAGAGATGGGCATTGTGAATAGGGTCAAAAGAACCACCAAAGATAATAATTTTTTGCTTCAAAAAAGATCTTAAAACATTTAATTCTAACTAAAATAATTGTGACACAAGAGCAATGATCCAAAAGGAGATGGAGATCTATAACCTCTTTACTTTTCAAATTGATCTTGATAAAAAACTGTTGTTTGAAAAATCAAATGATCAGAAGAATTATTCCAAGATTAGAACCCATTATTTCAAGCATAAATTCAAAAATAAAAGTGCTGTTTTTCTCAATAAAAACCTGATTAAAAATAGCTTAAATAAGGTTCTTTTAAACTTTTCTGATTTTGTTAGTGGTGCTGGAATTGATACAGTTTTTAACCAAATCATTGATGAAGATCCTGAGGTTTTAAACTATTTAAAGCAAGTTAAAAAAGATCTTTCCAAAGAAAACAACGCTACATCCCAACTAACCTTTAATGTAACTATTAACCCTAAAAACACCCTCGCCAATTTTTTTGAAGGATTTAATATTTATCTCCATTTTAATGAAGAAAACAATACAGTGATAGGTAGTTTCTCACTACAATGACACATCAAAAAAACTGATCTGTTTTCTGAAACAAAAAACATTGCCATTAACAATTTAATTCACACTTTTTGTAAGAACAACATGCATGAAATTAGTTTTATGCAGATCATTAATTGTTTTTCCAAAACAAAAATTAATAAACATGGTGAAATTGTTTTAAAAAGTTGTGCTTTTAAACAAAAATGACAAAATGTTGTTGCTGAAAAATACCCATTCTCAACTGCATCTAAAGATTTGGAGAAAATTAATGATTTTTTTGATGCTCTTTTTGTAATGCTATTATTGGTATGTCACCTCAATAAAAACCTGTTATGATTATGTGAAAAAACTGATTTTTTTGAATGAAAACCAAGTCAGAAAACTGCACTTTTTAAAGCTAATGATAGTGGAGCATACTTAGCTAGAATGCTGCTCTTTTTAAACGATTGGTACAACGAAAATCAAGCAATAACAACTGCTGACATTGAAAATGTTAATGAGGTTGAAGACATAGGAAAATTAGTTGAAAAATACAGCACTAATCAACCCCAAAAGTTATCGTTAAATTCAACAGTTTATGTATTACAAACAAAACAAAAACAGTTTTTTTTAAAAAATGATTTCTTTTTTAATAATAACGAAGCAAAGCTGTTTTTTCTAATAACTATGAAGCCCAATGTTTTTGGTCTTGATGATACAGCAATAGCAAATAACTTAAACCTTAAAAAAATTAGTGATTTTTTTAAAGAGATTGATTTTAATGATGAGGATATTCTAAATGACTTTAAACAAGAACAGGAAAAGTTATTGGTTAGAAGAACATTTAACCAACTGTTATTTATGAACAAAAATACTGAAATTCTCAGTGTTGTCAATGACAAGCAAAAGTCAGTAATCCATAACATTGTTTGAACCATTACTTATAGTAAAGCGATCATGTTAAAAGCCTTTGATTATTCCAAAGCCTTTGAAAAAAACCGAACGAGTGATCCTTCCTTATTGCGATCTAATTTAACTGTTATCAACCGCTTAAGATACCTCAGTGAATACTTTCAAAATGCCTCACTTAAGTATGATCTACTCTACACTAAGGCCAAACAATATATGCAAATTGATAAATTTATCAACGACATGATTCGCAAGGTAAACCATGAGGATGAAATCTTTGGCAAATTTAAAGAAAGAATTTATTTAAGTTTGGGAATTATTAGTGCGGTAGTGTTTGGCATAGTTGAATTTTTTAACTGTGTATGGACTATCTTAACTGTTAGTCAAGAAGTGGTTGATAAAAGTGTTTTGGATCCAAGGAATATTATCTTTATTAGTATAGGTACTATTTTGGTTTTATTTCTTTTGGTTACTATCTTGGTTTTTATGACAAGAAGACTTTATCTGTTTGAAATTAATAAAAAGCATAAAAATTAGGAATCATGAAATTCAATAAGTTAAACCTAAGTCATTGTATCTCTTTTTACATCTCAGAAGTTTCAGAAGTTTTCTTTGAAAGTATCAACCAACACCCTAGTCGTGATTTTGTTAATAACATTCTTCAAAAGATTAAAACTACTTTAAGTGAAGAAGAGCTTGAAAAGTTAAACAGTATTGAAGAAGTTACTAAGGATGAGAAGATTGTGATCATGCTCAACCATGTTTTGAAAAAGATTGTTTCTAAAACTGGTAGTTCTAAGTGTGACTTATTTAATGTAATCAAACAAGATAGGTTTAATTCTCCAGTGTATATCCAAAGTATTAATGCTTTTGAAAACAACCTTATTAACAATGAATTTGCTGAAAGAAGGTATGACTATTTGATTGAAGTTAATAAAAATTCATACCTTAAAAAATTTGTCAATTCGATCAGAATTTCCTTCTTCTTAGATTTAAGAGCACAAATTTTGTCAGGTTCATTTACCCTTAATTTAGTTAATAAGTCAATTGAAAAACAAAAAAAGACAGAAATTTTTAAAGATATTTTTGTTAATGCCTTAGTTAAGCATTTTATCTGTAATCAACTTTATCCTATCTCTTTAAATTCCTTTATTTTTGACAGTGAAAATCCTAGCAATAAACTCGCTCTAAAAGAACGGATTAAGCTTTTAAAAACAAACTGAAATTCCCTCTTTTTTGATAAGTTTTACAACTGTTTAAACAATAAAAATAAGCAGCAACTTCAAGAAACTAGTGATGAGATGTTTTATGCAGTGATTAACACTTATTTAATCATGTTAATCTCTGTTGAAGAGTTAAGGGTTTACTTTACAAGTAAAGAACCAGCACTGATTTTAAAGGTATTAGATAAAAAAAACACACTAAGAGAAGATCCTGATCAAAACCCTGAAACTGATTTGTATGAACTAATACAGTTTATAGAACAAAACTACTTAAAAAAAGATAAAAAAACCAGCTGAAATAAAAAAAAGGTACAGGATCTTGAACAGTTACTTGAAGAGATTAACAAGATTAATTTAGAAACAAAAAATGAATCTTTAGCTTACCCTGATGAGATAACAGAATTGGAAATTGATAATGATAATTTTGTCTCTACAAAACAAGTATTTAGAAACCAATTAGAATTACAACTTTTGCATGGGATTGTAATTAATCCTGAGAAGTATGGAATTGGCATGTGAAGTAGTTATTTTGCTGATTGAAGTGAGTACAAAAATTTAATAGAACAGATGCTCAATCCCAAAAGTGGTAATGATTTTTATCAGTTTGAAAAGGACATAGATGAAAGCATATGTCAAATTAACAAAAAATATCTAACTTTTATTAGCAGTGATAGTAATACCTTTTTAATAGTTAAAAATGATGATGTAAAAGTTATTTCTAACTATGTATGAGCACAACTTTTCTTTGAAACAAGAAGGTGAATTATCAATGACATTGAATTTGACTTGTATGAAAAAGGATTTGATAAAAGCCACTTTTCACGTAACATTGCTTTACTTGAAAGTCTCAGTTTTAGTTGATTAGATCCTTTTTATGGTTTGACATCAATTAAAGAGATCATGCAGAAAATAGATAGTAAAAGTAATCTTAAAACCTCGATTGAAGAGATGGTAAATAGGTTTAAACATGAGCAGCGAATTAATAAAAAGGACAATGAAAGGGTGTTGATGATTTTTGCTTATATTGCTGCTTTTGTAGTAGGATTTATCAATTTTTTCTCAATGGTCTTTACTATTCTCACTGTAAGTGATCTAAATGCTGGGCTTACTGTACCTAACATCATTGTAATCAGTATCGCTAGTGTTTTAGCTTTTATTTTGATTGTGATTGCTGTTTTATTTCGTTTTAAATGAAAACACATTAAGCACTAATAATGCAAATTAAAGTTATTAATGAAACAAATAAAACTGTTCAAATTTTTCAATGTGCTAAAGTAAAACATAGGGCATTAGCTTGATTGTGCGATGTATTTTTATTAGCTATTGTACTTGTGGTTATCTTTTTAATTACTCAAGCCTTTAGTGATAACCGTTTTCTTTTATTTTTAGTTTTAAGTTGTTCACAAACAATCCTGTGAACGGTTTATTTCATTTTTTTACCATTTTTTTGGGATGGTAAAACTTTGTTTCGCAACCTTTTGAAGATTAAGCTATTTGCTTTTGACAAAAGGTTTTTGAGAATAATGATCCATGAGTTGTTTTTATGGATTTTGCTTTCAGTTTTGTTTTTAGTGATTGCTAGTTATTTTTTTATTAACCAAAATAGCAGCGAAGTGCTTAATTTTTTCACTAATTTAGATAAGCCTAATGCAATAGCGATAACAATTAGAACCATAACAATTTTGATTAGTTTTTTACAACTGATTTTTATTGGTTATTTTTGTTTTAGTTCTGAAAAACAAGCTTTACAAGAGATACTGTCAAACACCTTTATGGTTCAAGAAAAACACACTTTAAAATCAAAACCAACTAGCTTAAAAACCAACAATCAACCTGATCCAGCTAATTTACCAGGCGTAATAGCTCTTGATGAAGTGGAAAAATTCATTAATTAATAACAATGAATGAACAACAAAAACAAGCAATTAGTTGTGGAAAAGGGGTTAATGTTGTTTATTCTGGAGCAGGTACTGGTAAAACAACAATTATTACTAATCGCTTTGCATACTTGGTTAATAAAGAAAAAGTTGATCCTAGCAGAATTTTAGCAATCACCTTTACTAAGAAAGCTGCTAAGGAGATGCAGTTTAGAATCTTGAAACTAATAGATAGTTCTTTAGCTGAGAAAACAAATATCTATACATTTCACAGCTTTTGCAATAAGTTTTTAATTCAAACATTAAAAAAGCGCTTTATCATCGATGATGATATTAGCTATTTCCTAAAGGAATTTTTAGCTGATTCAAAACTCGATATCAACCTAGCGAAACAAATTATTGATAACTTTAAAAATACTTTTGCTGATTTTGAAATAAATAAGTTGGATCAAGATGAAAGGTTAATTAGTTTATGTGAGCATTCACTTCTAAATAAAGATGAAGAATATTCCACTTTAAAAACCCAACTGATTAATGCATTCATTAGCTATGAAAAGAATAAGATATTAAACAATAAACTTGATTTTCATGATCTTTTAATTAAAACTTGTAATTTATTGAGTAATGATAATGATTTACTTAATCAGTGGAGTGAACAGTTTCAGCATATTTTAGTTGATGAATTTCAAGATACCAACCAAATCCAATATGAACTGATCAAGATGTTAGTAACTAAAAATAAAAACTTGTTTTTGGTAGGTGATAATAACCAGATGATTTACCGCTGAAGAGGGGCGGTAAACGGGATCATAACTGCTTTAAAGCATGACTTTAATGTTCCGAAAAGCAATGAATTCTTTATTAATCAAAATTACCGTTGCGATCAGAATATTTTAGCAGTTGCTAACCAAATTCTTTTAAAAATTATGGCCTATGAAAAACAAGTTAAAACTGAAAAAAATCTCTTGTTTTCAACTTTAAATTCTGATAAAAAACCTGTTTATTTTCAAGCTGAATCAGTTGAAAATCAAGCCAATTGGATCTTCAATAAAATCAAAGCACTAAACCAAACAGAAAAGATTAATTTTAAGGATATGGCCATCTTGTTTAGAAAGAACAGAGATATTACTACTATGGTTGAATTGATTGAAGCGGATGGAACAATTCCCTTACCTAAACAAAAGAGTTATTTTAACCAACTAGTAAAACTCCAGCGGGTTTTAATTGCGATTTCAACCAGAACAAATCTTGATATTAAAAGAGCTTTGCAAGCCCTAAAAATTTGATCAAATGATTTAAAGGAATTGTGAAAACAGAGTGATAAAACAAACCTATTTGATTTTCTTAAATGATCAGAATTAAATCAAAAAAACCATAGTTCAAAACTTAAAGCTACTGGTTATTTTAATCTGCTGATTAAGTTAGCAGAGGATCAGCAAATTAACCTTTTGTTTACTGAACTGTTTAAAAAACTCAAAGTGGATCAAACTATTGAAAATCTGCTTTGAAAAAAACTAACTGAATTTCAAAAAGATAAAACTGAATTTAGCTTATCAGAGTTTATTACTAGCTTAGCATTGGAATTTGACTCAATTATTGAAAACAGCAGTGATACAATCAATTTGCTAACCGTTCATGCAGCAAAAGGACTTGAGTTTGAAGCTGTATTTATTTATGGCATGAATCAAGGGGATTTTCCCTTATTTTTAAGTCAAAATCAAAATGACGAACAACATTTAATTGATGAATTAAAACTGTTTTATGTTGCTATCACAAGAGCAAAACGTTTTTTGTTTATCACTGCGGTTTTACAAATAAATAACAATTCTATAAAACCATCTAGTTTTTTAAATTACATCAATAAAAGTGAGTATTTAGACATTGCTACTATTAACTATGTATTAGAGCAGGATGATGATTTTTTTGATTCAACTAAAAAAACAGACTATACAAAGAAACTAAGAAAAGAAAGTTTAGACATTATAGTGGGTGATTTAGTTACTAGTAGATACTTTGGAAAAGGAGTTGTAGTTGAAGTGAGAGACAAAGAGGTTTTAGTAGCTTTTAAAGACACACGCTATGGGATGAAATGGATCTTAAAAAACCATAAATCACTAACAAAAGCTTTATATTAACAATGGTTGATAAAAACAGTTTAAGAAAATTAATGCTTCTAAAAAGAGCAGAACTAAATGATCTTGAAAAATCGCATTTAGATCAAAAGATTAACCAAAAATTAATGGCTTTTTTAATAACAAGACCAACAATTAAAAATTTAGCACTTTACATTCCCATTAAAAACGAAGTGGCTTTTTTAGATAACTTTCTAGATTTTCTTAAGTTAAATAAAATTACAAGCTGTTTTCCTAGTATTGTTGATCAATTTAACATGAAGTTTATTGATCAAAATAATAATGAAATTAACCCTAATGATATTGATTGTTTTTTTATCCCTTTATTAGCTTTTAATAAGGCAAACCACAGGATTGGTTTTGGTAAGGGTTATTATGACCGTTATTTATCATTAACTAGCAAAAAACAACTAAAAATAGGGATAGCATATGACTTTCAATATGCAGAATTCACTAATGATCCTTGGGATTATCAATTAGATTTAATTATTTGCAATGGATAACATAAAGGTTCTTTTTTTAGGTGATGTTTATGGCAAAGCTGGTAGAAAGATTATTAGTGATCATCTTCCCATAATTAAAAAAAAGTATCAGTTAAATCTAATTATTGCAAATGCTGAAAACACTACTAATGGTAAGGGTTTAAGTTGAAACCACTACCAAATACTAAAACAAGCAGGAATTGATTACATCACTATGGGTAACCATACCTGGTTTCAAAAGCAAGATTTAGAACTTGTTTTAAACCAAGTTGATGTTATTCGCCCACTTAACTTAATGCAAGATTTTAACTATTTTCAGCTTGGCAAAGGGAGTTATTTATTTAGCTTAAATGGTTTGAAAATAAGGATTACTAACTTGTTAGGAACAAGTATTAACTTACCATTTGCAATAACAAACCCATTTGTGGAATTAAAAAAGTTAGTTTTAACTAAAGATTGTGATCTTCATATCGTTGATTTTCATGCTGAAACAACTAGCGAAAAAAATGCTTTTTGCATGGTTTTTGATGGTTATGTTACTGCTATCTTAGGAACCCACACCCATGTTCCTAGTAATGATTTAAGAATCACTCCTAAAGGAAGTGTTTACATTACTGATGTAGGGATGTGTGGTCCTGGATTTGGTAGTGTTATTGGTGCTAATCCCAAGCAATCAATTAAGTTATTTTGTACAGGTGAAAGACAGTTTTTTGAGGTTTCTAATTGTGGTGCACAACTCAATGGCGTCTTCTTTGAAGTTTGTTCAAAAACCAATCAAGTTGTGAAAATAGAACAGATTAGAATAGTTTTAGATGATGAAAAGTATTTAGCTAATGACTACTTTAATTTAGTTGAGTAATCTTGCTTTCCTTACCATGTAAAATTCTGATTATGTTTGCACGGTGTAATCAGAAAACAACCACAGTTAAAGGTCAATATCACAAGCAAAAAAATAAAATGATATACCATTCATTTTGATAAGTAATCGACTTTAGAGGATCACTGTTAAAGAAGTATAAATAATCAAGTCAGGGTATTAAGATGATTACAGCTAACACAAAGAATGTAATAAGACTTGCTAAAGAAACATACTTAGTTATTAAAGTAATCATTATCCAAATTAAAAGACAGATTAAAAATCATCATAAAGATATTGCAAGTAAAGATCCACCAGTAGTAGCAATTGCCTTACCACCCTTAAACTTAAAATACAGCGGAAAGATATGACCTATTGTAGCTGCAAAACAACTTAAATAACTTAAAAAATAGGTGCTTTGATACACTTTTTCTGTTAAATAACCTTGTAAACCAAAACGGAATAAAATTCAGGTTAATAAAAAAGCAAAAAAACCTTTAAATGCATCAAAAATAGCCACCAAAAAACCAATTTTTAAGCCAAAAACACGCATTGAGTTAGTAGCTCCTGGGTTTTTTGAACCAAATTCCCTGACGTTTTTCTTGAGTATTTTGCTGAAAATATCAGCAAAAATAATTGAACCTAACAGATAACCAGAAACTAGGCTAAAAATGACCAAAATGGCAATTGCACTAGCTTGATTCATAGCTTGTAATTATCTTATTTAATGTTTCAATTATCTTAAGATCCATCAAGCTAGGTTTTTGTTTGGGTTGATAAACTCTCAATAGGCATTGGTAGTGTTTCATTAAACAGTGTTTATCTTTAAAGTTAATTAGCTTTGGTCCTAAAATAACCAATTCCTTTGTTAATTTAAACGGTTTTTTTAGTTTTTCAATAACCAAAATAGGATATATAAACTCTCGATCCTGAACTAGTGTTTCATTAACAATATCCCAACTGTTTAAACTTAAAAAACTACGTAATTCAATTAAATTAGATTGGGGTTGGATTACAAAACGATTAATAAAGTTTTCTTTTTGGCTAATTATGTTAATGATTTTCAATCCACCAAGTCCAGCAATTACCCCTATTTTGGGATTAATATTTAGTTCTGGAAGGTTATTAAATCCATCACTAACAAAGAAGTGGATATTATTGTTATTTTTAAATTTTTGGTAATTACTTAATAAAGCATTTTTACTAATGTCACTGTTAACAATGGTTAGGTTTTGATTAGTTTTTATTAGATAGGATGTTAGATAGGAATGATCACAACCGATGTCATACACTAGCTTAGGATTAAATGATTGAACTAAATTAGCAATTGTGCTAATCCTTTTTTTCATTAATTCTTAGAAATGACATACTAATAGGATTGTTTCTAACTGCATGTCTCAATTTTCTAATTGCTTTGTTTTCAATTTGTCTGATCTTCTCTCTAGGGATCAAAATCTTTTGACCTACTTCATCAAGTGTTTTAGGTTCATTGTAAGGGGGCATGCCAATCCGCATTCTAACAATTAACTCTTCTTGTTCAGAAAGATTATTGTTCAACAATTCATCAATTTTTTCTGAATTTGAACGGCTTTCGGTAAACTCGTCAGGAGTTTGAGCGTCTGTGTCTTTAACAAAATCACCAAACTGGGACTCTTCATCATGTCCAACTGTTTTATCAAGCGAAACTGGATCTAAACTTAACCGTTTAATTTCAGCAATCTTTTTAACATTAAATCCTTCAGCTTGTCCTCCCATCTTTTCAGCTAACTCCTCATCAGTAGGTTCTCGCCCTAACTCTTGATACAAAGCCCGTTCTGCTTTAGATAAGCGGTTAATGGTTTCTACCATATGAACAGGGATCCTTACTGTTCTTGCTTGATCAGCTATTGCTCTTGTAATTGCTTGTTTAATCCATCAAGTAGCATAAGTTGAAAACTTATTCCCTAAAGATCAGTTAAATTTGGAAATAGCTTTTAAAAGCCCCAAATTACCCTCTTGAATTAAATCATTAAAATCCAACCCTCTTTCTAGGTGTTTTTTGGCAATAGAAACAACTAGTCTTAAGTTTGAAGTAACCAACTGATTAATTGCATACTTACGTGACTCTTCATCAGTACTATTTAAAACCTTGGCAATCCGCTGTTCAGATTCAAAATCTAACATTTTAGAAAAGTCAAGTGATCCTAAAAAGAACCTAACATTATCATCAACCTTATCACGGTTAGAGATGTTCTTACTTGTTAACTCTTCAATATCTTCATCAATAATTGAAAGGTCTTGGTTAGCACGAAATTCATGGATGTGCTCTTCAACATCGTGTTGGAGTTGAATCCCCTTATCACGTAGTTCATCCAAAACATAGATAATTTCATCTTCAGGTAGTTCAAACTTAGCGAGAACACTAATAATTTCCTTATTTGAAAGCGTGATGTTTTTTTGTTTCTTTTTGCTTTTCTTTTCTCAAGCTTCTGTTAGAAGGTTGATGACTGTGTTGGTGTCATCATTTTCAATGTGGCTTTTTAACGATCCTTCTAAGATATCAAATAACTTCAGATTATTTTTTTCTTTTAAAGGAGCGTGTTTAGGTTTTCTACCCCTTCTTTTTTTAGGAACATCACTCTCCTTATTTTCATGAAGGGTTTTGAGAAAAGCGATGTTTTCATGACGGTGTTTGCGTTGCACTTTATTGTCAACATAAATCATCTTTTTGTAAGGACGATTCTTTTCAAGAATACGCTGTTTTTTAAGTTCAGCAATTAATTCTTCAGATAGTTCTGGTTTGGTTGAATTGGGTTTTTCGCCTAGCGTTTTTTTGTCAGTGGACATTTAGCTCAATGCGACTAGCATTATAATTTTAGCTTATATAACTTTTATAGCGTTAACTTTAAATTTTTTAAGCGCTGCTTTTGTTGTTTTAATAACGTTTGAAAGGTCTTTAGATAGTTTTGAAAATCATTAGCTTGATAACAAGTTAATTCATCTAAAAAAATATCTTTAATTTCCAATAACCTTGCTTTATTGTTTAAAAGGTAATTTTGATCTAAAACACTTTCAAAACCAACTCAGTTAGCAGTTTGATTTTCACTCCAATAGATCCTTGCTTTTTCAATAAAAAGTTCAACATCAAACAAATTAAAGTTACATTCCTTAAAAACTTCATCTAGATGGGATTGTTTTAATTCAACCAAAAAGCGTTTATCAATTAAAGCAAACGCAAAGATTTCAGGTTTTCACAACTCATTAACTGCTTCTTCAAAAGCTGTTTGCACTAGTGCTGCAGTGGTTTTTTGCAATTCTTGTGGTGGTTGAGCATGGCCTGAAGTATTTATGTAAAAACGGTTGTTATCAAACTTAGATACAAGTTTTTCTTTGTGTTTTAAAACTGTTTCATACAGAGTTTTTTCATCACTATATTCAAGTAGTTTAACCAAATTTTTAATGAGAAAAATTAAAAAACTATGGTCGTTTAAAATTGTTTGGTTTTTTGTTAAAAAAGCAATGATTTTATTAGTAATAACCCTTTGATCAAGTTGTTGCTTTTTAAAAAAACTAACAAGATATTCAATTAGATTTTGTCTTTTGTTTGCTTGTAATATAACTTGCTCACTACCCTTGTTTAAATACAGTTCATCCCAATCTTTATAGTTGTGTTCTCACTGAACAATTTCCACAATAAAATTGTTGTTATTAAGTTTTTCAATTAAGCTAAACACAGCATTTTGGCCACTAGCATCATTATCAAGTGCTAAAACTAAGGTTTGTAACTCCTTAAAGTGAGCTTTAATGGCTTTAATTTGCACATCATTTAATGCTAATCCCATTAATGCAACAGCTTCAAACTTGGAGTTTGTTAGTGTAAAAACATCAAAATAACCTTCCACAATAAAGAGTTGATTGAGGTTTTTATTTAACCTGTGAAAGTTAAATAACAGCTCCCCTTTTTTAAAAAATTCGTGATCAGCACTATTTTTATATTTCAGTTTGTTGATGTTATCAACACTTCTTGCTGAAAAACCAACCGGGTTACCATTAAAGTCATGGATAGGGATCATAATCTGGTTTTGAAAGGTAGCTTTTTTGGTATTAAAGTCAAAAAAGCCAAGACCTTGCTGGTTAGTTTTTGAAAAAAGATACAATTCACTCGGTTTTATCTTTGGATTAATGAAGGGGTATCTTTCCATACTTTCACATAGATACTTATCTTCATTGTGAAAAGCAAGTCCTAGCTGAAACTGTTCAATTAATGTTTTATTAAGCTTTCTTTTTTCAACTAAATAATTCATCCCATTTGGGTTTGTTTCTCTTTTTAATCTGGTTTGATAATAAGTAATTAAAGCATTGTTTATCTCCCAATATCGTTTTTGTTTTGGATCAACTTTTGTTAGTAAATTACTGTTCCAATTTTCTAACTTAATTCCACAAATTTCAATTGCTTTTTTAAGTGCAGTTTTTCAATCTAACTGGTCATGCTTTTGGATAAACGCTATTCCGTTGCCAGCTGCATTACAAGCCCAACACTTAAAGATGTTTTTAGAACTGGAAATGGACATAGAAGGATTTTTATCATCATGAAAAGGACATAAAGCAAGTAGACTATTACCCTTAGTTTGGATTTTAACCCCGTAGTGTTGAATAATTTCAGTAATTTTAATCTGCTTTAAAAGTTCATCTAAACTGTTGCTTTTATTGACCATTTTTCATGAATCATTGTGGTAATTCTTTGATAGCAATTCGCTGTTGAACTAAAGTATCTCTATCTCTGATGGTAACTGCATTATCTTCTAAACTTTCAAAGTCAAAAGTGACTGCATACTTTATTCCAATTGCATCTGCTTTTCGATACCTTTTACCAATACTACCAGTTGTCTCAAAGCACACTCTTCACCTTGTTTTTGCTAGCGTTTCAAATACAGAAAATGCTTCTTTTTTAAGTTTATTTACAAGTGGTAAAACAATAATTTGTTCAGGACATAAGTCAAATGGTAAACTCAATACTTCCCGTTCTTCCTCATTAATAATTTCTCTCCTATAACTACTGACAATTAGTGCATAAAATAACCGTTCAATGCCAACCGCAGGTTCGATTATGAAAGGTAAGAAATGTTGGTTTAATTCGCTATCAAAAAAACTCATGCTCTTTTTTGAAAACTCTTGGTGTTGTTTTAGATCAAAATCACCCCTGTTAGCCAAGCCTCATAACTCCTTTAATCCGTGGGGAAAATTAAACAAAAAGTCAGTAGTTTTTTTGGCATAATGAGCCAATTCTGATTGATCATATTCATGTTTTTTTAACAATTCTGGGTTAATTTTTAACACTGTTTGTAGAAACTGTTCTACCATTATTAATTGTTTTTCAAACAGCGAATTTGCATCATCAGGTTTACAAAACCACTCAATTTCAAACTGTTCAAACTCTCTAGTTCTAAACAAGAAGTTACCTGGGGTGATTTCATTACGAAAGCTTTTACCAAACTGGGCAATAGCAAAAGGTAAATTACGCTTCTTAGCTTGCAAGATATTTTTAAAGTTAATAAAGCTACCTTGAGCAGTCTCAGGACGGAGAAAAACAAGGCGTTTATCGTTGTTTACAACCCCGATTTCAGTTTGAAAAAGTAAGTTAAAATCCCTCACTTCTGATCAATTTTGGTTATGACAATTAGGACAGTTAACTTTAAAACTATTCCAATCCTTTTTAGCTTTTATTTCAGCATTAATTTGATCAACACGAAAGCGGTATTTACAACTCTTACAATCAACTAAAGTATCAACAAAGTTAGCTAAATGTCCTGATGCTTTTCAAACCAATTCGCTGAGAATAATAGGGGTTTCAACTAAAAGAACATCAGCTTTATTTTTAATAAAAAAGTTATATAAAGCTTGTTTGATTTGTTGTTTTAAAACTGCACCTAATGGACCAAAATCCCAACTATTGTTTAAACCGTTGTAAATTTCACTACTCTGAAATACAAAACCATGTTGTTTGAGAAACTGAACATAAACTTCTTGGTTGTAAACTTTAGCCATTGTTAATTAATCTTAAAAATTAGTTTTTTCAAGCAAACAAGATAATCCCTAAAGCAACACTTACATTTAAAGAATTGATCTTATTGTTCATAGGAATTTTGATTCTACAATCTGCATTCTTTGTTATTAGTTGATTCACACCCCTATCCTCATTGCCAACAATTAAGATTTTTTTAGCAAAATCAACTTTGCGATAATCAATTGGTTTTCAAATAGGATCCAGAGTTGAAACAACAGTTCAAAATCCTATTTCTTTTAGCTTAGTGATCGTATAACTTAAGTTAGCTACTTGCACTAAGTTTTGATAAAAAACACTACCCATACTAGTTTTCATGACTGTATTGTTGATAGGGACTTGGTTATTCTTTTTAAAGATAATTCCATCAACCTCACTAGCCAAACAAGTTCTTAGTATAGCACCAAAATTATAAGGGTCTTGGATCTCATCTAACATCACAAGTGTACTGCACTTCTTATTTTCAACTACTTTAACCAGTTGATCAAGTGGAATTAAGAGTTGGTTAGTATCTAAAACTGCTACCAATTCCTGGTGGTTAATATCTCTATACTGATTGTTAAATCAGTTAGTTGAATGGATTTGAAAATTAATTTTTTTAGCTTCAATTAAAGGTATTAGTTTTTTGTGACGAATGGAAATGTTTACCAATTTAATGTGAACTTGGTTATTAATCGCTTCCTCAAAGGCTTTAACACCAAATAAGCAACTTTGTTGGCGTGGTTTTTTCATTATTACAGCAACATTTTTTTAATTAGTTTTTTTCTAATTTGATCAGCTTTATCCATTGCTTTTTGTTTGTGTAATATTGATCACTCTTTAATTAAACGCACATTAAGTTTAGTATGGATAGATTTAAAGCTAATTCCTAATAAATCAATTGATCATTCCAAGAAACTAAGTTGTTCTCTCAGCACACTAAAGTCCTTAGTTTTAATACTTGTATTAATTTTTTTTATTAGTTTTCAGATAGCAGCAACTGCATTGGCAAAGTTGAGATTATCAAGTAAAGCTGAAAAAACTGGATCATATTGCTTGGGACTAATCAACTCAGATTGTTCTGAATAAACTAATCAGGTTCTAGCAACATTAAGTGTTTTTGCAATCCTTTGAATATCATTATTAGCTTTTTCAATCAATGATTGGTTTAGATCAATAGGATGCAAATAGTGTTTTTGGTAAAAGATCCAACGCAAAACACGAAAATCATGAAAGTTAAGAAAATCAACTGCTAACAAGAAGTTCTGCAATGACTTTGACATCTTTTGGTTTTCAATCATCAAATGACCAATATGCATCCAATGTTTGGTAATGGGCTGGTTATATAAAGCCATGTGTAAGGCATTTTCATTTTCATGGTGGGGGAACTTTAAATCAACTCCTCCTCCGTGGATCGTGAGTTCATTTTTGAAACAATAATCAATTAAGAAGGCACATTCAACATGTCAACCTGGTCTGCCAAGTCCCCACTTACTATTTCATTTAATCCCTGCAGTAGTAATCTTTCAAAGTACAAAATCCAATTTGTTCTTTTTATCAGTATCAGTTTCTTCTAAATGCACCATTCGGTTAGCTAGATAACCATATTGCTTTAGTGAATTAACTGCAAAATAAACGTTGTTTTGAGTTGAAACATAAGCATGGTTTTGATTTACTAATTGATCAATATAGTCAGGGATTTTATCGATTTTTTCAGTGATTTGAATATGTTTAATAGGCAGAATATTTAGTTTTTTTAAAAGTGATTTGTAAGCAGTGATTTGTTGTTTTGTAACAACTGATTCGCTTACTTCTTGTTGTTGAGCAATCTTGATGATCTTATCGTCAATATCAGTGATGTTTTGAACAAAATTAACGGTATATTTAGCCTTTTTTAAAACTCTATTTAAAACATCAAAAACAATTAATGGTCTGGTGTTGCCTAAGTGCAAATCGTTATAAACTGTGGGTCCACAGAGATAAATGTTAATGGTTTTTTGAACTAGTGTTGTTGGTTTTTGACTAACACTGTCAACAATCATAAACCTATGTTCAAATTGCTTCATTAATGATAGGAATGTTCAGTTCTTTGGCGTGATTGATTTTTCTTAAAGTTGGTTTGTTTCCTGCAATAACAAAGTCAACTGTTGGTTTGACTTCACTTGCAAACTGGCAATCAAACTTAGCTGATAATAAATCCTTAATTTGGTCACGACTAATGTTAAAGCTACCGGTAATAAGAAAGCGTTTTTGAAAATAAATACTGTTAGTTTCAAAGTTAATTTTCAGTGGTAATTGATCTGTTTTTACTTGTCTTAATTCAAGTTGTTCAATTAACTGCAAATGGTTAGGGTCATGAAACCAGTTATACAATGATTCAGCTACTGTTATTCCTACATCATTTAAACTAATTAAGTTCTCTAAGCTAGCATGCTGTAATGCTTTGATATTTTTAAAATGATTAGCTAAATTCTTAGCTAATACATTACCAACATGCTTAATACCAAGTCCTGTTAGTAATCTAGCCATTCCTTTTTGTTTTGAATTTTCAATGTTATCAACTAACTTGTTGAAAAGTTTATCACCAATCTTCAGATCTAATTTTAAAACTTGGTTTTTCTTGTCTTTTAAATCATAAAGATCAACTATAGATCTAACCAAATTGTGTTCATAAAGTTTGGTAATAGTATTAATATTCAACCCGTTAATGTCCATAGCAGTTTTAGAAACAAAATAGTTAATTAACTGGATGTTTCGCTCCTTACAAGTTTCATTGGTACAATACTGATCAACTTCATCAACTATTTTGACTAGTTTTGAATTACATGAAGGACAATATTTTTGCTCTTGAATTATGATAGTGTCATTTTTTCTTTTTTCAAGGTTTACCTTTAGTACTTTAGGGATAATTTCCCCAGCTTTATAGATAACAACAGTGTCATTGATCCTAATGTCTTTAGTTTTAATGTAATCAAAGTTATGTAAAGTAGCAGCTGTTACTTTTGTTCCATCTAAATTAACACTTTCTAATTTAGCAGTATAGTTCACTCTACCAGTTCTACCAATCGTTATAAGAACTGCTGTTAATTTAGTTTGAACAAATTTAGGACTAAATTTAAATGCTATTGCCCAACGTGGTGATTTACTTGTAGCACCTAATTGTTGATAAAAAAGCAAGCTGTTTAGTTTAATAACTAAACCATCAAGATTAAAAGTTAACTGTTCTCGTTTTTTGTCAAACGCTTCCAAGTAATTAATTAATTGAAATTTATTTTGAAAAACCCTGATGGTATCACTAACTGGGAATTTTCACTGCTTAAGCTGTTCTAAAACCATAGTTTGAGTAGTGATTGACTCTTCCAAACCATTAGGGATGTAATAAAATAATGCCCTTAATTTGCGCTGTGCAGTGATTTCACTGTTTAAATTACGTATTGTACCTGCTGCTAGATTCCTTGCATTAGCAAATGGTTTTTCAAGTTGATTGTTAATTGCTAAAAAAGTTTTTTTATCAACAAAAATCTCACCCCTAATCTCAATTGTTTTTGTGAAAGGGATTGTTAAAGGGATAGATTTAATGGTTTTAACATTATTTAAAACATCTTCCCCAACACTTCCATCTCCTCTGGTTAAAGCATGAACTAAAACACCATTTTTATAAGTTAGAGAGATACTAACTCCATCAATTTTAGGTTCAACTACAAATTCATTTTTTGAGTTTGTTTGAAAGTTAATATTATCAATAAAATTAGCAATTTCTTTAGTTGAAAAAGCATTTTCCAAAGAGAGCATTGGACTGTTATGGTTTAACTTTTTAAAACCCTTCACAGCTTCTCCTCCCACCCTTTGGGTAGGGGAATCAATTTGGATTAAATCAGGATGATCTTTTTCTAATTGTTGGAGTGACTTATACAACATATCATACTCAAAATCATCAATTAAAGGTTCGCTTAAAACATAGTAGTGATAGTCATAGTTTTTTATTAAGTTAACCAGCTGTTGAATCTTCAATTTCACATCCATCACTACTATAAATAAGAAAAATCCTGACTTAGTTGATACCAATCAGGATTTTACAAAGAAGTTTTAATTTGGAGCAGATAACGGGAATCGAACCCGCATCTTAACCTTGGCAAGGTTATGTTCTACCATTGAACTATATCTGCGTTGTTACTTTAATATTTAGTATATTTTAACTTGCATGGAATCTGAAAACCAAATTGCAATCCTGGATTATATTTTTAACCAAGTCAACCAGCCCAATCAACCAAAAATAGTTTGGTTCTCTGGGGAAGGGGAGGATGAGAAAATTAATTTTTTAATCCGCTTAAATGATTTTTTTAAACCAAAATTTGTTGAAAATACTAATGATAGTAGCTTTTTATTAAGTTTTAGAAACCATGTTGAAACTAAAAATTCAACGCCTTTAACCCAAGCTAACTTTGCTAATATTGCCAACAAATTACTAGCGGTTTTATTTGGTTCATTGCAATGAAAACAGTTAAATAAACCAACTGGAAACTGATTTCTTGTAATTTTGTTTTTAGCTTTATTATGGTTAAGACAATGCTGACTAAAACTTCAGTTAACTAAGATAAGTAAATTTGTAAACCAAAAGGGCATTTTGAGTTTTATTAAACAACAATGGCCTATTCTAACAACATTGGTAACAGTAGGGACCACATTAGGTACTCCAGTATTTTCATTAACAATAGCCCAACAAGATGGTATTAAGCAAAATGCGGGGAATGATGTCTTTATCTTTTTGATTATCTTCTCTGTTTTTTCTATTAGTTTAGGGCTTGTTTCATCACTTATCTTTTTAGTCTCCAGCTTATTTTCAATTCATCAGAAAAAAACGTTGGATGCTTTGGATAAAGTGTTATCAAAATTTATTGATAAATACTTTTTTTTAGATGAAAAAGAGATCAAAAAACAACTTAAATATCAGTTTAAAAACAACGGTGTTTGCTTTTTTTATGGTTTTGATTTTGATCAGGCAGAGTTTCTTGAACAATCAATGAACTTAATGTTGTTGTTAAAGCAAACCAATTGTTTTATTTTAGTTGGTTGTAAAGAAAGTGAAATGACGCTCATTAAAAACAAGATAGAACCTAACATTAACTTAAAACAAAACAGTTTTTATCTGGATTTAAGCAATGAAATTTCACAAGTAGAACAGATCAGCAAATTTAACTTGTTGTTTAGGCAATTGAGGCTCAGTTCTGAACTATTTTACTTAGAAGATTTTTTTGATTATCTAACTACTGCAAAACAGATAGTTAACTTTCTTTTTAAAACAAAACCTAACCTTGATCAATTTCAAGAAAATAAAAAGAAATTATTTGATTTTTTAGCGTTATGAGCATTAGTAATTGGTACTGATTTTGAGTTTAATAACGTGTTGTGATCATTTAATAACCACTTTGTAATTGATAACAGTTTTAAACAGGAATATGACAAACCCAACATTACTGCTTTCTTTAATCGTTCTTTGCAATTTTTTCAAGAAAATAGCTTGGTTTTAAAACCTGAATTGTTTAGTTTGCAAAAATACACAAAGGATGTTTATGGTTTAAATGTTATCAACCAACTAAACTTAAATAAGCACCCTATGCTAACTCCATTAACATGGGATAAAAAGCAAAAGTTTATTAGCTTTATTGAAAGCTGTGTTCAAAAATACAGTCAAGTTAAAAAAGATAACCAGGTATTTAGTTTAACAGTTGGTAAGCGCGTCTTTTTTCTATTATTAATAAATAAGCAGTTTAAACAAATTAAACTTGAAACAGCACTAAAGTACCTTGGCTTTAAAACTTCCCTTGGTGCAATGGACAGCACAACTGAGTCCTAATCAGTAAATAGAGGTTCAACTCCTCTAGGGAAGGCCAATTTATGATAAAAACTTAGTTAAATTGCTAGGTTTTTATCTAGTTTTAGGGGGTGTAGTTTAGTGGTAGAACAACAGTCTCCAAAACTGTCTGTGTGGGTTCGATTCCTTCCACCCCCGCCATTTACAGATGCACTTCAATTCAAACTTTAAAGAACGCTTTAATAAGATTGCAAAAAAAGTTAATTCACTGGACAGTGAATACTATGAGTTTTCTTCTTTTATTGAAAGGATTAGAACTACCTTTGGACTTTTAATTGCTTTAACTGTTTTAAGTAACCTAATCATTATTAGCTTTGTTTTAATTTGATTTTTTACTGATGGCTTTGGTCAGTTAAGGCTGTTATTCTTTACGCTTTTTATCCCCTTTTTTATCTCTTTATTAGTAGCAATCTTTCTAATCTTTCTTAACAATTCATTCCGTAATTTTTTTCAGATTAATGAAAAGAACTGGCTGTTCTTATGGACCTGTGTTTTTTCCTCATTACCAATCTTTAACTTGTGGTTAATTGTGAGATTAAATAAAACTATTAAGAATTTCGCTAGTGATTATGGTTTTAAGATTGTTAATAAATATAACAGTTTAACAAGCGGAATTTTTGTGTTTGACTTTGCTGATTATGTTAGTTTTGAAGCCAACCTGACTAATTGAAAAAACACAAACGATAAAAATCGTAATTTTGTTAACTTCTTTGAAACAATAAGCAAAGAAAAAACTGGTGTTGTTCAAAAACCAGTTCTTAACTTTCAAAGATTATATGTTAATCGTTTGTACTATCAAAGTAAGCTAAGTGTTGGTAGCAACCAACAGACTCCCCAAACTGCTTTTGATAACCTAAGAAACTATGTTGAAAACAAACAGCGTGAAACAGTAAGGGTTAAGCAGTATATCCTCACTTAATTAAGTGAATATATTAATATTTATTGGTCTTTGTCAAAAAATAAGATGAAAAAGGCAATCCACTTTCAGAGTCAACCAGTTGTTTTTAACTGTGCTTCATGCAATAGCAACTTTACCATTGACTCCACTGCCAAACAAAAGGATCTTGCCATTGACATTTGTGGAAAATGTCATCCTTTTTACATAGGGCAATTAACCAAACAAACCGTGCATGGACGGGCTGAAAAACTTTCTCAAAAGTTCAACGCTGGAAAGGCTTTTTTAGAAAAAAAAACTAAAAAGAGTAACCAAGCTAAAGTTGAAAAACAAACTAGGCACCGTTCTATTAACGAGCTTTAGTGGATTTTGACAAACAACTCTTTTTCAATGTTGAAAAGATTGTTGAACTTACTGAACAACTTGAAAAAGATCTCAATAAACCTAACCTTAGCTTTGAGCAGATTAAAGTTATTAACAAAGAGTTAAAACATAAACAACCTTTAATAGTTAAATTCAAGGAGTTGCAAAAGCTGGTTGAAAATGCTAATGAAGCTGAACAAATTCTTAACAATTCCAGCTTAAAAGAATTGCATGAAGAAGCTAAAAAAGAACTTGAAAAAATCAAAGCTAGTTTACCTAGTTTAGAAGAGGAGATTAAGTTCCTTTTACTACCGGTAGATGAAAATAACCAAAAGAATGTTATTGTAGAGATCCGTCCAGCTGCTGGTGGGGATGAATCTTGCATCTTCTTAAGTGATCTTTTTAATATGTACAAAAACTATTGTACTAGTAAAAATTGAACGGTTGAACTTAATGAAATTATCCCTGCAAGTGTGGGGATTAACTTTGTTTCTTTTGCGGTAAATGGTACTGATGTTTTTGCTAAACTCAAGTTTGAATCAGGAGTACATAGGGTGCAACGTGTTCCTTTAACAGAAGCTAAAGGTAGAGTGCATACCTCAACAGTTACTGTTGCTGTTTTACCTCAATTAGAAGAGGTAGAGATCACCATTAATCCTAGTGATTTGAGAATCGATACTTATCGTGCTTCAGGAGCGGGTGGTCAACATGTTAACAGAACTGAAAGTGCAGTTAGAATTACCCATCTACCTACGGGAATTGTTGTGGCTTGCCAAGAAGGTAAATCCCAGTTTTCTAACCGTGATAAAGCAATGAAAATGCTACGTGCTAAGTTATGGGAAAATGCTCAAAATAAACAACTCTCAACCCAAGCGGATTTAAGAAAAAGTCAAGTTGGTAGTGGAGAGAGGGCTGAGAAAATTCGTACCTACAACTATCCCCAAAACAGAATTACAGACCACAGGATTAAATTAACTATTAATAAGCTTAATACTGTTATTTTGGGAGATCTTGATGAGATCATTGAAGCTTTACAAGCTGATGAAAAGAAACAACAGTTGGAAAAGTTTATTAGTTAGATGACCCTGTATGAGTTTTTTTTAAATCAAAAGTTAGTTTACCAATCCAGTCCCCATTTTAACGGGGTATTTTTAACAATATTGGAACACTATGGTTTTCAATTTAAAACAATTGATAAACTCTGAAAAAGTAAGCTTCTAATTACTAGTGAGTTAACTGATAAAATCAAACAACAATTAAAGTGTTATTTTATTGAAAAGATCCCTTTGCCCTATTTGTTGGGAACAATTCAACTAAGGAAGCTTACTTTTAAAACTAAGAAAGGAGTTTTTATTCCTCGAATTGATAGCTTAGCACTAATTGCAAGTGTTAACTTAAAAAAAATAAAAACTGCACTTGACCTTTGTTGTGGTTCAGGTACTTTAGCCATTGCTTTAAAAAAGAAGTGTGATACACTTGATGTTTATGGTAGTGATATTGATATCCAAGCATTAAAACTAGCGCAACAAAATGCATTAATTAATAACGTTAGTATTAATTGAATTGAAGCAGATTGATTTGATTGTTTTAACAAGATAAAAACTCCGATTGATTTAATTGTTACAAACCCACCTTATCTGAAAAAAACACAACTAAATAAAACATTAAATTATGAGCCTAAGCACAGCTTGGTTTTTCAAAATAAAAATAGTTATTTTGCATACAAGCAGTTGTTTAATCTATTACTAACAAAACGATCAATTAAACAGTTAATTTTTGAATGTTCTTTATTTCAAAAAGAAAGGCTATTAAATTTGTTTTCAATCTTTAAATCAAGGCCGATTTTTAACTTTCAAAAACAGTTTATTGGTATGAAAGTTGATAATCAAAAACTCCCAGTAGTTGATATTAAAAATACCAAAACTATTAAGCAACTTTTAAAAATGGGGCTAGCAGGAATTGTAAATACTGATACACAAATGGGATTAATTAGTTATTCAGAGTCTACTCTTGACAAAATTAAACAACGTGCACTTAACAAACATTATGTATCAATGTTTGGGTTAGAAGAATTAAAGAAGTTACCAAAAAAACTACAACAAATTGCTAGTTACTTTTGACCAGGTAGTTATACCTTTATTAAAAATAACAAGAGCTACAGGGTTCCTAAAAACTTGGGCTTATTAAACCTTTTTAATGCAATTGGTAGGGTTTTTTGTACTAGTGCTAATATCAGTAATCAAAAACCATACACCAAATTAAGTGATTATCAAAACGATAGTTACTGAATAAAGCAACCTTGTTTTATTATTAGAAGCACTTCTAAAGTGCAATCAAATAACACACCTTCACTTGTCTATAATTTAGATACAAAACAGTTGGTTCGCACCACAGCTAAACAAACAAAACAGTTTCATAAATTAATAACTAAACACCAGTTAGCTATCTAATACACAAACCAAAATGAAACCAAAGATAATAACAAGCTTTTCAATATAAAAATGCTTAATTCAGTTTTATTTTTGAAATAAAACTATTTTTTTTAATTAGTATATAGCATCCACTACTAAAACATAAGGGATGAAGCTCAAAGGTTTTTTAGCTGTTGGTGTTAGTGTTTTTGGTTTTTCTGGTTTACTGATGGCTTGTAGTGTTGTAAGTCAGTTTGATCAAGTGGATGATGGCAAAATTAAGCTAGCATCTTCATTAACTTCGAAACGCGCAGCGGAAGCTTTAGAGACAGTAGTTAAAAAATATAACGATACTAAAGATCCTGGTGATTATCCAATTGAAATAGTACAAATTGCTGGGGGTTATGATGGGGGTAAAAAGGATGTTCAAACCAAGGTTAGTACCAAAGATAAAAATAACTTTTATAACCTGATTTTAAACTATCCAGAAATAGTAAGTACCTTATCAAGGTCTAAAATGGCTTTGAATTTTGATGGGGTTAATGTTGATAAACTCCATCCTAACTTTTTAAGCTTTAACAGCAGAATAGGTGGAATTAGAGATGATGGAATCTATGCTATTCCAATATCTATGTCCACTGATCTGATGGTCATTAATGGCCCTGTTTTACACTATATTCTAAACAGTGCTAGAAAAGAAGGTACACCAACTAGCACTACTGTTCAAGCAACTGTCAGTTCAAGAAGTGCAGAAAAAAAAGGTACATTAGAAATTGCAAATGATAGTGAAACTACTAAACTTTGACAGAACATCCAAACCACTGCTCAAAACAACAGTAATGAAACAACTAAGGAGCAAAAACAAGTAAAAAGATCTAGTAGTTCTTCATCTACAACATCTACTACTGGTGAAACTAAAGATACTACAAAATCAGATAACAAGATTAAAGAGTTTTGGGGTGAATATCAAGAAGTGGATGGAGGGTTAAAGAATTTTACCTTTAAAGCAAGCATCTTTGAAAACTGAAATGAAACGTTAGATTTTGCTACTAGAATAGCAAACTCTTTTCCTGAAAAGGTTAAAAATATAACAAATAAAACTGGGCTTGATTTACAAGGTGTTTTAGGAGTTGATAGTAGTTCTAATGCACTTTATGCAGCAGTTTTTGCAGCTGGTCAAGCTAACTATGATAACTTCTTTTTTAACATCGATAAAAGAACTGGTTATGCAGATTACTCTAACTTTTTAAATAAAGATAGTTCATACCAAAATTTAGAGAGTGTTTACAATGACTTTTATAAATTAATCCAAGCTAATGGTTTGTTTGTTAACCGTGGTGGTTCCTATTCATCCAACTTTGAAAAATTTCACCAATTGGCATTCTCAGTATCTTCTTCTGGAGGATACAGTTATTACTTTGCTAAAGATAATGCTAAGCGCTTAAAGTTTAGTAATTATGCTATTGAATATCCTAGTTTTACCCAAACAATTCAAGCTCCTAATTCTTCAGAAACAGAAAGTAATTTACTTGGTACTTTTAAATTAAGTGAAAAAGATATCAATCTATATAAAGGTTCAATTCCTAGTGGAAAACAACAAGGAGTTGATGCTATCTTAATTAGTAACCCAAACTTAATTAATATTCTTGAACAAGCAAAACAAAAAAACACTGCACAAGGAAGTGAATCAACCACTAACAAGATAATAGGTTATACCACCACTGCAAATGTTAATGTTGATAATCAAAACATCTTTTCTGTTAGCAAACTTAACAACGAACAGTTTCAAAGAAAAATCATTGTTAATGCCACTGAAGAAACACTTGATCAATCCCAAACCTTACAGAGCAATGAATCAATTGTTTTACCAATGCCTGGTAAATACAAATCAACTGATAAAAATAAAGTAATGATCACCCAAGGTCCTAACTTAATAGGCATCCATGCAAATGAAAAAGAAAATATTGAAACTAAAAAATTTGTTAATTGGTTTTTAAATCAAAGTATTACAGATTGGAATAGTAATAATCAGCAAAAAAATAGTGATCAAACAACAAAAACTGCTGCTGAATATTTCACTGATCAAGCTTCTTACATCCTTCCTTTAAAGGAAAAATTTAACAAAAGTTCAGATTTAGAATTGAAAGGCAGTAGTAGTTCTTCTAATTTAACAACCAGTAGTGCTAGCGCCTCTTTGTTAATAAGTAATAATAGTTCAACTGCAAGTTCTCCTGCTCCTAAAAAAACAAACAATAATTCTAATACCTTTACAGCTAAAGCACTAGAATTATTCCAACAAGCTGCTAACAATGAAATTATTCCCTTTAGTGATCCAAGTGACTTTAGGAACGGCACATTCCGTAATAATATAAGCAGTAGTTTTAATGCTGCGGTGAATTCTAAGGTTAGTTTTAATCAATTTGTCCAAAACTTTATTAATAGTTTAGGATCTGGATTTAGAAGATAATTAGTTAACCTCCCCCAACCTCTATTTTGTGTTACTAGTGCCTAAGGTGGTAGTGGAGTACCACAAGCTCAGTAAGAAGGTTGTCAAAGAGAGTTTGGAAGTGGAAGCAACTGATTCCTTTGATCCCACCCAAAGGTTGCAAAAAGATAGTCCAATGAAGGATTCAACAAAAACAGGGGAGAAACTCCAAGAGACCATGTCATCAATGAGTGGTATGGCTACATCCACTCGCAAGGCCCTCAAAGTGGAGGTGGAAAGGGGGAGTAAAGTCAATCAAGGCGAACTAGCAAAAAACGACTTTGCTAAAAAGCCACTGAAACATAAAGAAAATAGTGGGGAAGTGAAGTTAGATGCAGAGAAGGATTTTACTGAAGGAAAGGTTTGAAAACCGGTGTTGACTACAGATGAGATAGAAAAAAATAGGGGGATGGGGGCGACTTAGACTTTCTCCCCTGAATCGGCAATGGTAAAGCTTTTTCCAACTCCCCCTCCCCTTCAACTTCTGCTTCTTCTACTCCCCCTCCCCACTTTTTCTAACATCAATGTCGGGGTTAAATCAATGATCACTCAACACTTAAACAAAGAAAACACCCGGTGGGTGTTTACCCCTAACTCTTCACCAGACATTTGAACGGGAGCAGGGTATCGCAAAGGTTCATCACAACAACAACGGCATCCCTTTTGATAATGTGAAGCCTAGTAGTAGTAGTAACTCGTTTAATCCCTCCTCTGCTGAAAATCAAGTCACCCCAGCTGGTGGCTCCTCCAAACCAACCACCTACACCCATTTACCCAACAGTATCAGTCCCACCAGTGACTGGATCAACGCATTAACCTTCACCAATAAGAATAATCCGCAGCGCAATCAGTTGTTGTTAAGAGCACTGTTAGGAACTATCCCGGTCTTGATCAATAAGAGTGGAAAGGGAGAAGAATTTACCAAAGATAGTGAGCAGAAGTGGGATAAAACGAATAAAAAAGATGGGAATTTACCGGGGTTTGGGGAGGTGAATGGTGCTTCTTATAAGATTTTTACTTATTTAATAATTAAAAAAAGTGTTAGGTTTTTTTAGTTTTTTATCTATTTAATATTTAAGAAATTCTCAAATTTTTCTTAGTTTTTTATTTGTTTAATAGTTAAAAAAAGCGTTATGTTTTATCTATTTTATTAGTTAAAAAAGTTTTGAATTTTTATCTATTTTTAGTTAATAAAAGTCTTATGTTTTTATCAAATTTTTATCTGTTTTTTGGTTAAAAAAGTTTTAGATTTTCTTTCTTAAATTTATTTATTAAATAGTTAATAAAAGTGTTAAGTTTTATCTATTTTTAATTAATAAAACCTCGACCCCTCTTCCTATCAAATCGCCGACCAAACCATCCATAACACCAACTTGTTTGTGTTGTTCAAGTCTAGGGATGTGAAGCTTACATACAGTTCAAGTGGCTCAAATAACCAGATTAGTTTTGATTCAACTAACAACAAACCCTCCTACATCGTCGAGTTTACTAACTCCACAACAGTAGGGATCAAGTGAAGCGTGGTGAAAAAATATCAGTTAGATGTACCAAATGTTACCAATGAGATGAACGATGTACTGAAAGAGTTGATCCTTGAACAACCTTTGACTAAGTATACCTTAAATTCGAGTTTGGCCAAAGAAAAGGGTAAGACACAAGCAGTGGTGCATCTTGGTTCAAATATGGCAAATCAGTGACAATCGATGCGTAACTCAACACATCTGAACAACAATCCCAGTCCCAATGCTTCAACTGGATTTAAATTAGACAAAGGCAACGCATATAGAAAACTAAGTGAATCCTGACCAATTTATGAACCGATTGATGGGACCAAACAGGGCAAAGGGAAGGATAGTAGTGGGTGGAATTCAACTGAAGAAAATACGGCAGCAGGGGATGCGCCCCTTGTTTCAACAAGTGGAACATCAGATCAAAGTAATAAATTCACCAAGTACCTCAACACCAAGCAAGCATTGGCAAGCATCGGCATCTTGTTTGATGGAAATGGGCGAGGAATGTGGTTAGCCATCTCTACCAACCCAACAAGGTGAAAAGTGGTCAATATCAACAAAATAACACCTACAACAAGTTAATTGAACCGGAAAGTGCAACAAGTGCAGCAACTAACATGACCAACTTGTTAAAGCTGTTGTCTAGTAAAAACATCAAACAGAAGTTGGGGAAGACAGCCCAATCTCAGGAAAATAGTGGGGGGGGGTTAGTCAAACGATTAACACCATTACCACTACGGGAAATATTAGTGGCAATGGAACCATTAAAACGGCTTATCCGGTGAAAAGTGATCAAAAATCATCAGTAGCGATCAATTCCTTGATTAACGCTACGCCCTTGAATAGTTATGGGGATTTAAATAATGCTAGTTTTTCTAAATAATTAAATTGTTAATAACAAAAAAATCTCTATTAAAAAAACCAACTTTAAAGTTGGTTTGAAATTCTAAATGGCGCGCCCAATAGGACTTGAACCCATAACCTTCTGGTCCGAAGCCAGACACTCTATCCGATTGAGCTATGGGCGCATATATTAATAAATTTTATTAATATAAGCAAGAACAATCTAATTCTTTATTAGAATTTAAACGATTTCATCTAATAGTTCAAATCACATATGGCAAAAGATAAAAAAAATAAGGTTGATGGAACAGAGCAATCAGTTGATCTATTTGAACGTACAAAACTTGAAGATACACAAGTTTTAAATGAAGTTGAACTTGATGATATTAAAAAGATAACAGAGCTTAGAAAAGAACTTGAACATACTTTTGAACCACAAACAAGAATGCAAATTAAGCGTGAAATTAAAGAAATAGAACGCAAAATGAAACGTTCTAGTCGCTAATTGATGTTTGTTAATTTACATACAAATTCATACTATAACTTTCTCAATTCTGCCCTTTCTCCTAAAAAGCTAGTTAATCTAGCAATTAATGATCAGCAAAAAGCTGTTGCTATTACAGATCCTAATCTTTTTGGCGCTGTTGAATTTTTTATAACTTGTAAGCAAAATAATATTAAACCAATTATTGGTTTAAACTTAACTGTTGAATACCAAAAAAATGATGTTAAGTTATTACTAATTGCTAAATCAAATAAAGGCTTTCAAACGTTGAACAAAATAGCATTAATTCAACAAAAACTTGAAATTAATTCTTTAGTTGATCAACTAACAGATATTGCAGTAATTATCTGTTCTTTAACAACATGAAAATCTACTTATAAGGATGTTTATCAAGCAAAAGGAATTGAAATAAATCAAACCCCGATTGCCATTCTTGCAAATGCTGTTAACTGTGAAAAAACTAATAGCGATCAAGTAGTTTTAACAGTTTTGAAACAAATGAAACAAAACCAAACGGGAAAAATAACTACATTTGATTGGGATTTTAAACAAAAATTAAATCAAATTTCAATTAATGAAAATTTAAAAGTAAAGAGTGAAATTCAACCTTTTTTAGATCAAAAAACTGCACAACAATTATTCAGTGAAACAGAACTTAATAATCTGAATGATCTAGTTAATAGATGTGAATTAGATTTGGAGCACCTAAAAGCTGCTTCACTTTCTTTAACTGATAATGATGCAGCAGTTTTAGAAAGTTTGTGCCAAACCAATTTAAAACAGTTTTTAGATAAAAATCAAGATCTAAATAAAAAAGCCTATCAGCTACGTTTAGAGAAGGAATTAAATGTTATCAATAAACTTAATTTTGCTAGCTATTTTTTAGTTGTCAATGATCTTGTTAATTATGCTTTTAAAAAGGACATCTTAATTGGTTCTGGTAGAGGTTCTGCAGTAGGATCATTAGTGGCTTTTTTATTAAACATTACCAAGATAGACCCAGTCCAACACCAGCTTATTTTCGAACGTTTTATCTCAACCCACCGTCAAGATCTACCTGATATTGATATTGATATCATGGAGAATAAAAGAGCAGAAATGATAAATTATCTGTTTGAAAAATATGGCAAAGAAAACTGTGCACAAATTGTTACTTTTCAACGTTTTAAAACCCGTTCTGCTGTTAAAGAAGTTGCTAAATTATTTAATGATTATGGCATTAGTGACATGATCCTAGGAGTGTTACCTAAAGATCAAACTATAACATTCACTGAGCTTAAAGCTACTGAAGATAGTGCTTTACAACTTTGTTTACAACAGTTTGGTTTAATTGTTGAATTAGCACTAGCAATAGTTGATTTTCCAAGACAATCAAGTATCCATGCTTCAGGCATAGTTATCGCTTCAAATTCTTTGATTAAAACCATTCCCTTGTTACAGCTTGACAATAATCACTTTTTAACTCAAGTTTCAATGGAATGGTTAAGTTTTTTTAATCTCAATAAGTTTGATCTGCTTGGTTTAATTAACCTTACTATGATTAGCGATGTAATTACCCAAATTAAACCATCTAACCAGACCGTTAACCAGTTTTTAAATACCATTTCTTGAACTGATCAAAACACCTTTATAAACTTAGTAAATGAAGATACACTAGGAATCTTTCAACTTGAATCGTTTGGCATGAAAAAATTACTGGTTCAGATTAAACCTAAAACCATTAATCAACTAGCAATTGTTCTAGCGCTTTACAGACCAGGTGCACAGGATAACATTAACCTTTTTATTAACCGTTTGCACAATGGTTATGATCAATCTGACATTGATCCTAGGATTTTACCCATTGTGAAAAATACCTATGGAGTTTTAATTTTTCAAGAGCAGATCATTAACATCGTTAAAGTTGTGGCTAACTACTCTTTAGAAGAAGCAGATAGCTTCCGTAGAGCCATTTCTAAAAAGGATGTTAAATTGATCCAAAAAAATAAGCGTAACTTCTTTGAAAGAGCAGTTCAAAATAACTTTGATTTAAAGACTACTACCAAAATTTTTAGCTACATAGAACGCTTTGCTAACTATGGGTTTAACCTTTCTCATGCGTTGGGTTATGCACTGCTTTCATACTGAACAGCTTGACTTAAAACTAACTATCCTGTTTATTTTTATTTATGGTTATTAAACCATTTTCAATCTAGTAAAGACAAACAAAAACTAATTATTAGAACTTTAGAAAAAAGTGGTATTGAAATTTATCCACCTCTTTTAAATAAAGCTCAACCAAATAGTGTTATAGAAAATAAAAAAATTTATTTAGGTCTAAACCTAATTAAGGGAATTAATGACAGGTACATCCAAAACTTACAAAAAGTGCAACATTTAATTCAAACTCAAAATAACTTACAACTAACTGATGTAGTAAGTTGGTGTTTGGATAAAACCATTGGTGATATCCCTTTAAAAGATTTACTTTTATTAAAAACTATGGGCTGTTTTGATTTTTTTGAATACACTTATGACTTTAATGATGCAAAGGATTTTTGAATTAAAAGCGATCACCTATTGTTTACCAGAATGCCTTTAGAAAAAAAGGATAGTAATTTTTGAATTAAACAATTTTTTACCAATTAGTTGAACATGAAAAAAGCAATCCTGATTGATGGCAATTCCTTAGCATACCGTGCTTATTTTGCAACGTGAAAACAAGTTGAATATGCTAAGCAAAATAATTTACCTTTCAATAATGCAATAAGAACAATGTTACTAATGTGTTGGAATTTAATTAAAGCCAATGTTTATCAATATGGAATTGTAAGTTTTGACACTAAAGCGCCAACTTTCCGTGATCAAATCTATGAAGGATATAAACAAAAAAGGGTTAAAACTCCAGTTGAACTTTTAGTACAAATTCCTCTAATTAAACAAGCGCTTGTTTATTTAGGGTTTTTAGTTTGTGAAAAAGATGGCTTTGAAGCAGATGATTTGATTGGTAGTTATGCCAATTTATTTACAAAGCAAGAAATAACAGTTGATATTTACAGTTCAGATCGAGATATGTTGCAATTAGTAAACGCCTTTACTAATGTGTTTCTCTGTGTTAAAGGTACAAAGGAGATGGTTATGTACAACAATGAAAATTTTAAATCACTTTTTTATGGTTTGGCGCCCTATCAAGTTGTTGAATATAAGGGGTTAGTTGGTGATAACAGCGATAATTTAGCAGGGATTAAAGGGATAGGTCCCATCAAAGGGATAGAATTACTCCAACAATATGGAACCATTGATAACATCTACACTAACTTCAATAATCTCCCCAACCAACTTCAAAAACTTTTAAATAACCAAAAGGAAATAGCTAAAACCTTTAGTTTTCTAGCTAAAATTAAAACTGATATTGAACTTGATCAAAACATAGATCTTACTGGTTTAAAACCAATCCAAAAACAAGCGTTAATTCAACTTCTAAGTGAAAACAAAATTAATACTTTAGTTGAAAAATTTTCAAAAATATAATGCCTGAACTTCCTGAAGTAACTACTGTTATTAATGAACTTAAAGAAACTGTTTTAAATAAACCTTTAGATCAAGTTCAAGTTAACCTAAGAAAGGTTTTGAAAAATATTGATCCTCAATTGCTGAATAAACAATTAAAAAATCAGTTTTTTACTGATATTAAGCGTAAGGGTAAATATATCATTTTTCTTTTAAGTAATGGTTTGTATTTAGTTTCGCATTTACGTATGGAAGGTAAATACTTTTTTGAAGAAAGAGGTAGTAAATTTAATCAAAAGCATGTTTTAGTAGAATTTCATTTTGATGATGGTAGTCAACTCAATTATCATGACACCAGACAATTTGGAACGTTCCATTTGTATGAAAAGTTAGAACAAGCAGCACAATTAAATAAACTTGCATTTGATCCTCTAGAAGCTGGTTTTGACTATAGGAAAATCTTCCAAAAAGCACAAAATTCAAAACGTAAAGTTAAAACTTTTATTTTAGACCAAACAGTGATTAGTGGAATTGGCAATATTTATGCAGATGAAATCTTATTTGCAAGCAAAATTAATCCTGAAACAATGGTTGATCAACTAACAATTAAAGAGATAGAGATTTTATGTAAAAATGCTACCAAAATTTTAGCTAAAGCAATAGTTATGAAAGGTACTACCACCAGCAGCTTTAGTTTTAAAAAAGATCATACTGGAGGCTATCAAAACTTTTTAAAAGTTCACACTAAAAAAGATCAACCTTGCTCAGTTTGTAACCAATTAATTGTTAAAAAGAAGATTAATGGAAGGGGGAGCTATTTTTGTTTAAACTGTCAAAAAATCACAACCAAAGTTTCTACAAAACTCAATCCATAAATTTTTTAAAACTTTAAATATCTTAAAATTAACTTTTAACAAACTAATTGAAAACTTTATTTAACAAAGTAATGCTGTAATTAATCAATTAGTTAATGAACATTAAATAACATTTATGAAAAACGAAATTAAATATCTTTATTCTGACTTGGATGGTACTATTGTTAGCTGAAATCCTAAAACAGAATTTGTTTATCAAAATAAAAGTTATAAAAATTTCCATGAAGTTAGTGATGCTACTATTAGTGCTTTTTACCGATTGCAACAAAAGGGAATTAAGGTTGGTATTGTTACTGGTAGGGATTATTGTCGGGTGTTATGACTTGAAAAACAACTTAGAACAGGATTGCCTACCATTACTTTAGATGGGGCTATTATCTTTTATCAAAACGAAATCTTAAGTCAAACTTATTTAGATGATAGATTTATTGAAGGGATTAATAACATAGTAAAGCGCTTTCCTGAAGCTGCTTATAAACTTAACAGTGGTTGAATTAGTTACTTTACTAAAAACCCTTCTGTTATCTTTGAAATTGATTATGCTTTTCTTGGCTATTTCAACCCTAACACCAAACTACAAAAAAAGTTTATAGACAGTACTGAAAATTGAGATCTTAATAAACTAAAAGTTAATCAGGTTTACTTTGATATTGATACTTGCCCCTTAGCAATGCAAAAGGAAATAATTGAACTAATTAGTGTTAGTGATGTAAATGCCAAAATCTATGAGCACTCTATGTACATTATTAAAAATGGTGTTTCTAAAGCTAGTGCATTGCAAAGCCTTAACCAGTTTGCAATTCCAATAACAAAAGATAACACTATTGTTTGTGGGGATGGAGATAATGACATTGAAATGATGCAGTGAGCTAAACACAGTGTCTCACTAATCGGTAGTAATCCCAAATGCTTTGCTCTAGCAAAATACCACACTGATAGTGTTGACAATGATGGTATTGCTAACTGGATTGAAAAAAACTTGTTATGTTAATTGCAATCGTAGGTAAACCAGGTGTTGGTAAAACCAGTCTATTGCAATATCTCAAAGATAACTATCACTTTTCAGTTTTTTATGCAGATAGTTTTATCCATGAACAGTACCAAAAAAACAATCCAGGTTATCAATTAATCATGGATCATTTTGGCAAAGAGTTTGTCAATCAAACTGAAGTTGATCGTAAAAAACTAGCAAACTATGTTTTTAGTGATGATAAGTTAATCGAAAAACTTTCACTAGTAACAAAACCGCTGTTAATAGCGTGAATCAAATCCTTAAAAACCCAGTTTCAAAAAAAGCTAGCACTAATAGAGATTGCTGTGATGCTTAACTATTGAAATGAATATAGATCGTTGTTTGATTATGTGATTAAATTAGAAAGGGATGATCAGCTAGTTAACTTAGCTTTACAACAACGTAATAGTCATAAAAAAGTTAAGGATTTGATTAAAGAGCCTAATTGCAAAATAGATACAATTTTCAACAACGATTCGATTGCAACAGCTGCTTTAAAGCTAATTAAGTTGCTAGAAACTTTTTTAGAAAGAAATAAATGCCGTTGTGATTGTTGTCATATTCAGTAATAAACTTAGCATTTTTTTTAGCTAAATCAGTGCCATTAACCAAAGCAACTGGCCATTTAACACTTTGAAATATTTCAACATCATTATCAGCATCACCAAAGACCATAGTCTTTTCAGGATCAAGATTATAATTATCAACTAATACTTTCAATCCATAAGCCTTATTAACATCTTTTTGCATGATATCAAGAGCAAATGTCATTGAACTCACATAGTTAATATCTTGAATTTGATCTAGTTGTTTTGCTAGAAAAGGAACTTTTTCTCTGTTTTTAGTTACCAGTAAGATCTTTGTAATAGTATGTTGCTTAAAATCAAAATCCAAAGCTTTTTCAAACTTGTTAACTATGTAGTTATTTTCAATGAGATCAATTCCTACTATTTGACTTCTAGTTGAAAGAATTTTAGCTGAATCAAGCTCATGTGTAAAAGCAAAAACCTTTTTATCTGTATAGAAATAAAATGTTTCTTGAAATTCCAACAACATTTGCATAATTGCAGGTATTACTTTATTATCAATAGGTTTGATATGAACTGGTTTTTCACTAGCAAAATCATAAACAAGTGCACCATTGGAAGAAATTACAGGTAGATTTGGTGTAATAAAGGTAGCTGTGTTTCTAATTAAAATATGGCTTCTACCACTAGCAAAAGTGATTCTAATCCCTTTTTTCTGTAAATCCTTTAAAAACTCAACTGTTTGTTCTAATGGAATTTGGTTGCTTGAAAGCAAGGTACCATCAAGGTCAAAAATAATGTTTTTCAGTTCCATTTAGCTATATACTTTTATCTATTTTTTTAAAAGAACATTAATAATTCGATCCTGAACATAAACTACCCTCTCAATCGGTTGGTTGAGAATGTCATTTATCTCTTTATCCTGTTTAAATGATTCTAAAACATCTATCTCTAAACTACCTTTAGTAAATTCTTTAGCTGCTTTAAATTTGCCATTAATTGAAAGAATAACCTTAGTTTTAGCAGTCTCAAAAAGTTGATAATCAACTAAAGAAATCGCTTGTTTAACAACAAATGGTTCAAGTCCACATTTTTCATTCAATTCTTCAGCAAGAAAGGGCGCAAAAAACGACAGTACTGTTAAAAATCCCTTTGCATAATTTAAGCTAATTTTTTTGGTTTTATAGAGAAAGTTTAAAAAGATCATCATTTCACTAATCACCAGATTTAGTTCATGTTTGTCAAGATGACAATAACTGTTTTTTAAAAACAAATTGTAAGCAAAGGTTGTCTCTTGACTAACTTGATCAGTAACAACAGCATGATTAAAAAAGAAGTTATAAACTCGATCCAATCACCTTCTCATCCCGTTTAACCCTTCATCATTTCAAGTTAAACTAGCACTAATTGGGCCCATAAACATTAAGTACAACCTTAAAGCATCTGCTCCATGTGAATCAACAAGTGGTGTGGGGTTAATGGTATTACCTTTGGATTTGGACATCTTTTTACCATCAGGACCTAACACCATACCCTGATTAATTAATTTTTGAAATGGTTCTTTTGTTGATACTAGCTTCTTGTCAAACAAAAATTTGTGTCAAAAACGGGCATACAAAAGGTGTAAAACTGCATGTTCCGCACCCCCAACATAAAGATCAACTGGAAGGTATTGATCAAATAATTTCTTCGCTTCTTTTGAATCAATTGGTCAAAAATTAGGGTTTTTAATCAACATTAAATAACCCAGATAATACCAACAAGAACCAGCTCATTGGGGCATGGTATTAGTTTCCCTTTGGTAATGGATGTTATCTTTGACTATGTTTACCCAAGCTTGATTTCTCATTAGTGGAGAATTACCACTTCCATCTGGTTTGTAATTCTCAAGTAAGGGTAATTCAACAGGGAGTTGTTCTACCAAATGAGGAGTGTTATTTTCATCAAAAATAATTGGAAAAGGTTCGCCCCAATAACGCTGTCTACTGAAGATCCAATCACGCAGTTTATATACAGTTGTTAATTTCGCTTTATTTTGTGAAATTAACTCATTAGTAATAGCTACTTGTGCTTCTTTAGTTGTTAATCCGTTATATGCAAATGAATTTTGCAGCCGTTCTTTTTTATCAATGACGTTGATAATTTTCAACTTTTGTTTACGTGCGAAGAAGTTATCATTTTCATTGTGTGCTCCAACACCCATAATAGCATCTGTTCCATATCCTTCAATTACATAGTTAGCAACATAGACAGGGATCAATTCATTTGTAAGAGGGTGAATAGCATTTGTTAATAAATCTATCCCATCATATAAAGTTGCTTTTTGCTTTAAAGTTGTGGTTTTCTGGAGTTGTTTTTTTAAAAAACTAGCTACTTTTTTATTTGTTTCTGCTATCTTTTTTGCTAACCAATGGTTGGTTGAAACTGCAAGAAAACTAACCCCAAAAATTGTTTGTGGTTTAGTTGTAAAAATTGCAATAGCTTCCTTATGATCTTTTAGTTGAAAGTTAATAGTAACACCTTTACTTTTACCAATTCAGTTCCGTTGCATCTCTTTAATTGGTTCAGGTCAATCAAGTGTATCTAAGCCTTCAAGAAGTGCATCAGCAAAAGTAGTGATTTTCAAAACTCACTGTTTCATCTTGCGTTTTTCAACTGGAAATGAACCCCTTTCACTAACTGCGTTGCCATTACTATCAATTAAAACTTCTTCATTAGCCAATACAGTACCTAGCTGTTCACACCAATTAACATCAATATCAACTAATTCCGCTAGGTTTGCTTTAAACAGCTCACTGAAGATCCATTGTGTGTATTTGTAATAACGTGGATCAGTTGTTTTGAGACTTAAATGATAGTCATAATCAAAACCAAAACTAGTTAATTGATTAATAAAGTTATTAATGTTTTGATCTGTTCAACTGCCAGGGTTTTGATTAGAGTTAATAGCATACTGTTCAGCAGGTAAACCAAAAGCATCAAAACCAATCGGATGGATCACATTAAATCCTTTAGCTTTGTAATACCTACTTATTACATCAGTGATAGTATAAGCTCTAACATGTCCTAAATGTAATCCTGCTGCTGAGGGATAGGGGAACATGTCAAGGACATAATATTTCTTTTTGTTACTATCGCTTTCAAAGCGGTTAACATCTTTGTTTTTTCATTTTTTTAACCACTTTTCTTCAATTAAATTGTGATTGTACATCTAATTGCTTTGATAAGAACGCTTGATTGCATATTTAGTTAAGCGATAGAGTATGTCAACAATAATCGCAATAATCAAACCAAATAACAACCCAAAGCCAACAGGGATAAAAAAGTGAACCATTGTTTTAAATAAGACTGGTTGGTTTGCAACTGTGGCATTAAACCAACTGTTAATGTTGGGAAAAATCGTTAAAATAATCCCACTAGCATCACTGGAAGCGAGTAAATCAGCCCACTGAGCTGTTTGGTATGTATTAAGTCAAAAACTACCAATAAAAATAAAAAACCCAATTACCATCAAAAAAACTAGGATTGCAATTTTCACTAGTTTAAACAAAAGTGTCATTGTAGTTTTCAGTAAGTTATTTCAATTATAAAGTTAGATAAATTTTCAATCTAACTTTTGTAACTTTAATTTTTCAAGGATCATTTTAATAATAGTTACTATTGGTAGTTTTGCATCTATAATACAGTAATTAATCCCAAAATCATTACAAACCGCTTCCATAAATCCAGTGTAAACCCTGTGTAAATTTTGAAAGTAAAGTTGATTTTTAGTAAAGTTATCAATCTCTACTTTGCGGTTACGCATAAATAGTCTTTTTTCAAATAATTTTCAATCCCCATCCAAAATGACATATAAATTAGGAACCCCATGCTTATTAACTAGTTCTTTTGCTAATCTATTTCACAGTTGGTTATAGTATGAAAAGACTGCAGGACGAATGATGTTGTGCTTAGCAAACAACCAGTCTTCAAAGATAGAACGATCAAAGATGGTTGGATTGATAGTGTTGCAATTGGTTTGGTATTTACCAAAGCGATTAAGCGTAAAATAAAGCTGAAACAAAGGTGAATAGAGCAATTCATCACTACGTTCATACATCTTTGCTAGTAAAAGTTCAACCAACTGGTCATTAGTTTCCAATTCACAAACAACTTTTGCAGCTTGAATGTGGTTAGCTAATGTATTAGCAATGGTGGTTTTTCCTAAAGCAATCATTCCCCCGATCACAATACAATTAGCAATTTTATTTGGTTGAAAATGGGGCTTTTTTAGTTGCATATAAACACTTTTTAATATGTTGGGTTAAGGTTTATTTTTAATAAAAAACCATCACACTCACTGACAAGTCTTAATGCTGCTCCATTTCTGGCCTGACATGATTCGACTCTAATGATGTGATGGTTCTAAATTCTAACAATTATTTAAATCTTAATCAATTAATTTACTAACCCTGTTAATAGAATTGGATTTGGGTTGTGTAGTGCCTCTAGGCAATGAACTTGAATTTGATTTTTTAGATGGTTTGATCGGTTCATTCTCTTGATCTAATTGCTGTTGTGACGCTGGAGTTTCTTGTTGATAATTTGTTGGCGTTGAATGATCAAGTTTAGTAATTTCAGCTATCTTTTTTGTTAGTTTTGCTTTGGTTTTTTCAAACTGCTTAATGAAGTTAGTAAAGCCAGTCTTTGGATTCTTAGTTTCATCAAAAATGGTTGCAATAATCTCATCAAGCTTGGTGAAATCAATTTCTATTTCGTTGGTGAGTTTTTGGTGTTTTTGAAGCGAATTTACAACTAACTTATATGCTTTTTTTAATGCTTGCTTTTCTTTTTCAGCGGTTTTTAAAAGTGATTGGGTTTCACTAATTTCCCCATCTTTAAGTGCTAACTTGTCTTCAAATACGGTTTGGATGTGTTGATCACGTTTATAAATTGCTTCATTACACTGCACTTCATATTCCTTTAACAATTCCTCTACATTAATACTTTCATGTTCTTCAATTAGTTTCTTATTGGCTTCTTTTATTGCATGTAACTGTTTATTTAATGCTTCAATTTGGTGTTCTTTTGCTTCTACCAGCTGCTGCATTTGGATGATTTCACTTTCACTTTGTTTGAGTTGTGAGAAAAGTTCGCTGTTCTGTTGAACTTGACGGTTAATCTCTCCTTCCAAGCGTACCTTAAGTTGTTCTTGCAAACTTAAGACCCTTTTTAAACGTTCTTTTTCACTTCTCGCTTCAGGACCAAACTTTTTTAATAACAAAACAAGCATCTGATTCCAATCAACATTTTTTGGGTTGCTTGTTCGTAGTTTGACATAAGCAGTTTTTGCTTGTTCTAAAAAAACATCAAGCTGGGCTTTGGAAGCAAAGGGAAACTTGGCTAAAAAGAAGTTAATAGTTTCTCTTCTTTGAAAGTCATCAAAGTCTCTATAGTAATCAAAATTATTCATGGAAAGCTAATTCTCTAAAGAAGCTAATGATAGCATCAGGATCATTAAAGTCTAAATCATAATGGTGTCTAACATAAACACTAACAAAACTAAAGATTGCTAAAACAAAACTAGTAAAGAGAAAAAACGGGAGTTTATTGCCATGGTTTTTAACAAACAAATCTAACTTTTCATTAGCAATTTTTTTAAAACTAACTACATCACTAAACTGGTTAGGATCTAGTAAATAGTTGTTAAGATCACTAACTTTACTTTCAACCCATTCCAAGTTAGCTTGGTTATATTGAAATTCACTTTTAATAGTTGCAAAATCAGTTTTTTCAACCTTGTTTAAAATTGATTTGAAGCCATTTGTGTATTTTTGAACAAAACTATCCATTTTACTTGTTATCAAATATTACTTCTAAGAGTTGCTCAGGTTTTAAACTGCCAAAGTAATCACTAAAATGATCAAGTTCATTGAAGAGTTTTTCAAAGGTTTTATAATCGTACTTCTGACCAATTAGTTTTTTTGTTACTGGGGTGATATCAACAACACTTAAAAAGTCCCCATAAAACTTAATATCAACAACTGTTCCTTTCTCTACTTGAACATTGCACTCAAATAAACCAGCATTATTAAAATAACGCTTGTTTTTAAAGTTGTATTCATAAGTTTTACCAAAGTTTCACTCCCATGATTGAAAGTGTTCTTTTGCTCTTTTTTCAACCTTTGCTAGTGCATCTTTAGTTAAAACAATTGTTTCTGCTTTTTCAGTAACAGTGAAAAAATTAATCATCTCTTCTAAAAATTTTGCTGTTGTTCAATTTGGTAAATACTCCTTTACATTAACAACGCGCTTAGCAACACTGTCAACACCCTTACTTGCTATCTTGGTCTTATCAACATTTAAATACTTTGCTAACTTAGAAAAGTCAGTGTCAAACAATAATGTTCCATGGACTAATAACCTGTCTTTAGCGATATATTCAGCTAACCCAGAAAACTTCTTGTTATCAATCTCAAGGTCATTACGACCATGAAATACAGCAGGTACATTTAAGCTATTTAAGAACTTCACCACATTTCTTGTAGTTTGTTCATAAGCATTTTCCATCACTTTACCTGTTCTTGGCAAAATAATAGAAAAACAGATGTTACCAAGGTCATGAAACACCGCTCCCCCGCCTGAAAAACGTCTAAACAAGTTAACCTTATCACTTTCCAACTCCTTTAAGTTAACCTCAGCATAAGTATTTTGGTTTCTTCCCACCACAATAGTGTTAGCGTTCTGCCAAAAGTAGATGACCTTAATTAACTCATTTTTTCTAAATTCAGTTAGCAATCACTCCTCTAAAGCTGCATTAAAATACGGATTGAAAACAGGGGAAGTAATAATGAAAGTTTGCATCAACTACCAATTAACTAAAGTAATCAAAGATCGCTTTTTTACAAACATCAGTTACCATTTCATTCATGGTAGGATGGGGTGAGATAGAATTGGCAATATCAAACACAGTGAGGTTGTTTTCCATCACCAAAGCAAGCTCAGCGATAATATCACTAGCAGTGCTAGCAATAATACATCCACCTAAGATAGCACCAGTTTTAGGATTAAACATCATCTTGACAAAGCCATTGGTTTCATGATCTGCAATTGCTTTACCACTATAAATAAATGGCAAAGAAGATTTGACATAATCAATCTTTTCTTTTTGCAATTCCATCTCACTATAACCTACAAAAGCAACTTCAGGATTTGTGTAAATACAAGCAGGACACTTGTTTTTTTCAGCAGGCTTTACCTGGTTTTGGTTCAAAATTTGATCAACAGCATATCTGCCCTGTTGGTAAGCGTAGTGTGCCAACATCATTTGCGTGTTAACATCACCTATTAGATAGATGTTTGTAGTTGATGTTTGTAGTTTTTCATTTAAAACAATTTTGTTGTTATGGTCACGTTTTAAATCAAGTTGATCTAAACACTCTGTGTTAGCAATTCTTCCTATAGAAACTAAGATTTTATCACCAATTACAGACTGTTCAACTCCATTAACTGTGTAAAACAGTTGGTTGTTTTCAGCTCTAACAACATGAGCATTGGTAATAATCTGAACTCCTTTGTTTTTTAAGGTTTTACTTATCAGTTCAGAAACATCACTATCACAAACCTCCAAAATCCTATCAACACCTTGGATAATGGTCACTTCACTCCCTAATGAAGCAAATAAAAAAGCAAACTCAACCCCAATCACACCTCCCCCAACTACAACAAACTTCTTAGGTACTCCCTCTAAAGCCAAAGCTTGGGTTGAGTCAATGATAAACCCAGCTTGTTGTGCTTTTTCAAACCCTGGTAAAGTTAAGTATCTTGGTCTTGATCCGGTTGCAACAATAATGTTGTTAGTGGTGTAAGTTGTGTTGTTTACTTGCACCTTGTTTTTATCTATAACAGTAGCTTCACCTTCAATACTTTCTACCTTAGCACCCTTAATAATTGTTTTTACCCCTGCAACTAATTTATCAACTACTTCCTGTTTTTGTTTTAACAGTTGTTTTCAATCAAGTTTAGCTTGACCATTAATAGTGATACCATAATCTTTGGCATGAACTAAATAATCAATAATCTTTGCTCTTTTTAACAACGTTTTAGTTGGGATACACCCAACATTTAAACACACCCCACCAAAGTATTGCTTTTCAATCACTAGGGTTTTAAGTTTATGTTTGCCAGCATACTCCGCAGCAATATAACCAGCAGGGCCAGCACCCAAAATAATTAGATCATAATCCATAAATTAAGCTACTGTAAGATCAATTAATTCCTCAATTTGTTTTGCAATCTCCTTACCAAACCTACCAACATCCGCCCCATCAACCCAGCGGTGGTCTGCAGCTATTGTTAAAGGTAAGATGGTATGAACTGCAATTCCATTTTCCACTTTAACAATGCGTTCTTCTAAATTACCAGTAGCAACAATACACATCTCAGGGTACTTAATAATAGGTGTACCTACAGCTGCTCCTAATGAACCGAAGTTAGTAACTGAAATAGTACCTTTATTCAAATCAGTCAACTTAATCTTTTTTGTTCTAGCTTTGTTAGCTAAATCAACAATTGCTTGGGCAATTTCAACCACAGATTTGGTTTGGGCTTGCTTAATGTTAGGGACAATTAAACCTTCTTCAGTATCAACAGCAATTCCTACATTAATGTCATCATTTAAAACAATTTCGTTTTGATCAGGATCATAACTAGCGTTAAAAACAGGGAACTTCTTAAGCGCATTAACAATTGCTTTAACAAAGAAAGCAAAGTAAGAAATTTTCATGGAATACTTACTTAAAGCATAACCATTAACACTTTCACGATATTGTTTTAACTTGGTTGCATTAACATAAAAAGTTAATACAGTGGTTGGGATAATTGCGTGCGACTTTGTCATTGCTTCTGCAATCGCTTTGCGCATTGTTGAGATAGCAATGGTTTTCTGTTCAGTTTTACTTTCTGGTTTTTGGGTTGTTTCTACAGTAATGTTAGTACTACTTGCAACTTTAGTGTCTTTAACAGCTGGAGTTGCATGAGGTTTTACTCCAAAGATAGGAAAGAGGTTATCAGAAACCTTAATTTCCCCTACTACTGAAGCCCCCGCTTCTTTTACCTTAGTTACTTCTTCAGTTGGTTGAGGTTTTGGTTCAACAATTGGTGTACTAGTCTTTTCACCAATAACTGCCATCACCTGACCAATGCTAACAACATCACCAACTTTAACATTAATAGCGCTAATTGTACCTGCAAAAGGAGAAGGTAATTCGGTTGTAACTTTATCAGTTTCAACAACAAATAAAGCTTCATCTATCTTGATCTGATCACCAACTTGTTTTAAGATTTCAGTTACTTTTCCTTCATGTAAACCCTCACCAACATCAGTGAATTTAAACTCATTTGCCATATCGTATTGATAGTACTTAAAGTTTAAATTTAATAGTAGTGATCTATAACAAATAAAAAAAGCCAGTTTAGTTACTGGCTTTTTAGTTGTTAGACAAAAATTTTGTCTTACTTTTTACAGCAGCTGTTGCACTTAACGCATTCGCACTTGCATTGGCAGCATTTCTTGTTGTCTTCCATCACTTTAATTTATGAAATGAGGTACCTAGCTCTAAAAAAATAAAGCTAGGTAACTATATTTTAAAAAAGTGTTATTTTAAAAGTTGATTAACTGCATCAATAACCCGTGCATTAATTTCAAACTGGTATTTTTCACCTCTAGCTAAAGGCACAACAATATCAAACCCAGTTACCCGTTGTGGGGCTTTTTTGAGATAAGTGAATAGTTCTTCAGTTACTGAAGTGATAATCTCTGCACTTGTAGTGAAACTTTTCACCGCTTCAGTCACTACAAGTAGTCTTCCTGTTTTCTTCACTGAGTTAAATACTGTTTGTTTATCTCAAGGGGAGATAGTACGCAAGTCAATTAACTCAATTCCCTTATCTTTCAATTCCCCGCTGTAAACTAAGTTAATTAAATCAAACATTGTAGGACCATAGCTAACTATTGTAAGTTCACTACCTTCACTAATCAAGTTGGCTTCACCAATAGGGACAGTGTAATAATCACTAGGAATCTCCTGACGAAAAGCACGATAAAGCTTCTTTGGTTCAAAAAAGATAACAGGATCAGGTGATTCAATAGCAGCTAGAAAAAGTCCTTTGGTATCATAAGGATTTGATGGCATCACTGTTTTAAGCCCAGCAATCTGTGCATAAATTGCTTCCAATGTTTCACTGTGGTGTTCCAATGCTTTAATCCCCCCACCCATTGGCATCCTCACTACTAGTGGAGCGGTATATACACCACGAGAACGGTTTCTAATCCTAGCAGCATGGACAAAGATTTGAAACATAGCTGGGAATGAAAAGCCTGAAAACTGGATCTCTACAATAGGTTTAAGACCACCTATAGCAGCCCCAACCCCAATACCGGCCATAGAGTTTTCTGCTATAGGACAATCCCATACCCTTTCACTTCCATACTTTTGTTGTAAACCTTTAGTTGCACGGAATACACCCCCTTCAAAACCAGCGTCCTGGCCATAGAGTACAACGTTTTGATCTCTTTCCAGTGCAAGATCCATTGCGTTGTTTAACGCTTCAATGTTATTTACTTGGATTTTTGACATAGTTATCTTTTTTAATATTAGTCTTTAAAGTATTTTTTTGCAATCTGTTTCTGTTCAACTAGTTCAGGGGTTAATTCTTGGTAGTTGTAATCAAACACCTCATCTACTGATACAGGGGTATCTAGTACCATCTTTTCATAAGCAGCTTGGATTTCCTGTTCAATTTTGCTGAACATCTCTTCTTCTTGAGCTTGGTTAAGAATTGATCTATCAAACAAGAAGTTTCGCAATCGCTTCACTGGATCACTCTTCATTCCCTCCTCCTCTTCTTGTTTGGTTCTGTAGATAGAAGGATCATCTGAAGTGGTGTGAGGACCTTGCCGGTAGCTGAAGAACTCAATTAAGACTGGTCCATTACCACCTCTAGCGTAATTAGCAGTATCTTGCATCGCTTCATAACTAGCAATTAGATCATTACCATCAACCCTTACCCTTGGGATCCCACATGCTATTGCTTTAACACTTAGATCACTAACAGCAGATTCAAGTTTAGTTCTTGTTGAGATAGCAAACTGATTGTTGTTAATACAAAAAACAGTGTTTCACTTGTGGATGCTTGCAATGTTCATCGCTTCATAAAATTCCCCTTCAGCTGTACCTCCATCACCAATCATAGTAACAGCAACATTAGGTTGCTTTTTATAGTGCAACATGTAACCTAATCCAGCAGCATGGGAATACTGAGCACCAATGGTGATGTTAATAGGTAAAGTTTTGTATTTAGCATCTATCTGACTACCTTTTTCATTACCATTTCAGTAGAGTAAAAGTTGTTCTGGTTTTACCCCACGATACAACATTAAAGCCCCACTACGAAACGTAGGACAAACCCAATCATTTTCATTTAAACCTAATCCCATTCCAACCTGTAAAGCTTCCTCTCCCAAATTAGGAGCGAAGTTTAACATCTTACCAGCACGCTGTCAAACTAACATCTTTTTGTCCATCATTCTACTCAAGTTCATTAAGTAATAAGCGTGTTTTAACTGTTCATCAGTTAGGGTAATTTTGTGGTTAGGATCAATTAATTTACCCTCATTATCATAAACCTGATAAAGGGTAGTTGGAACTTTATTTTTAATCAAGATTGCCATATATTTCTATGCTAATTTAAAACTAAGCTATTTTTTAGGAATGTAATAGCTAAAACCAAGTGCTTGTAAAACAGTTGCTAACACAAAGTTAAACGGTTTGTTGTAATGTGGAAGAAAATAAACATCCACTAAACCCAGTTCAGTTAGTAACATCTGCTTTTGGATTGCAAGTGCAATATAGAAAATAATCTCACTGTGATTGGTATTCCAAGAAAGGAGTTGTGCTCCTAAAATTCTTAGGGTTTTCTTGTCATATACAAGTTTAAAACGCACCTTGTCATAACTGCCCATAAACTCAGGACGATCATTGTCATCAACAACTGATATGCCAACATCAAAACCTAACTTCTTAGCACGCTGTTCAGTTAATCCACATGCTGCTAAATTTAAACCAAAGATATGGAGTGCATTGGTGCCAACGATAGATTGGAGTTTAACTTGGTTACTACCAATGATATGCATCGCAGCTACTAATCCACTCTTTACTGCATTGGTAGCAAGATCGATGTTTTCATACTGTTCACTAGCAGCATTGTAAATAGCAGCACAACCCCCAATGACATAAACATCCTTATGATTTAGTGCTTGGAGAAATTCATTAACTTTAATTGAACCGTTGTGAATAAACTCAAAGTTTTGATCTTTAGGAACAAACTTAGTGCTAGGTCTAAACCCAATTGATTGGATCACAAGGTCTGCATTTACTATTCCCTTATCAGTTTCAACTCCTTTGACAACGTTGTTTGTACTATCAACAACAAAGCCCTTAACACTGCAACCCATCATTAGTTTTAACCCATCTTTTTGCATCACTTTTTTAAGTTCATCAGTAAACTCATGATCAAAGTTATTACCAGCAGGCTTATCAAGTAAGTCAATTACTGTTACTTGCTTTTTGCATAACCAAGCTGCTTCAGCAAGTTCCAAGCCAATGTAACCAGAACCAACAATAGCAACTGATTTAATGGTTTTATCTTTACGAAAACTATCGATTAAGGTAAGTGCATGTTGGTATAACTTACAGCTAATTAAGTTCTTAACATTACCACAATATTTGTCAGTGTAGTTAAACTCCAAAGGCTTGTGTGTCACCTTGTTTTCAACATTCATACATATAGGTCATGCTCCTGAAGCGATTACTAGTTGATCAAACTGATCAGTGAACTCTTTATTTGATGTTAAATCTCTAACTGTTACCTGTTTTTTGATTAGATCAATGTTAGTAACATCATGACTCATAAAGATGTTAGCGCCCATCTGTTTCAACTCCTTAGGGTTGGAATAGAAAAGATCATCAGTGTTTTTAACAACACCACTAACAGCAAGTGCAATTCCACACCCCAGAAACGAGATGTTTGTGTTTCTATCATAAGCGTTAACCTTAAAGTCCTTACTTTTTGAAAGTAAAGTTCTAATAAAACTAGTACCAGCGTGATTTATTCCAATCACAATCACTTTTTTCATATATCTTAGCTGCAGTAATTTTTTAAGCTATTTAAGGATAATTTGTGCAGTAGTGATTCGATCTTTTAAAATTAAGCTTTTGCTAGTAATGTAATAAAATCTTGCTAACCACATTTGGTTGTAACTTAGCTTTACCATTGAGTTTTTTCAGCTCAATTAAAAAACAATATCCCACAGTTTCACCATTTAACTGTTTAAGTAATTGGTCAATAGCAGCAACTGTTCCAGCAGTGGCAAGTACATCATCAACAATAACACACCTTTTAGCATTATTAGCTTGGATTAATGAAGTGGTTGACATCTCCAATACAGCATGTTTTCTGTACTCCAAATCATAGCTAGCACTAATTAATTGCCCTGGGAGTTTATTGGCTTTTCTAACCAATACTAACGGGAGTTGGGTTTTAGAAGCTAATGCTCCCCCAAAGATAAAACCCCTCGCTTCAGGACATACTATCGCTTCTGCATTAATAGCTTTAATAAACTGTGCCATTTGGGTTAGCACAAAATTAAATAGTTGGGGATTGGAAAATACTGGGGTAATGTCATAAAACAATGTACCTTGGTTGGGAAAATTTTCAAAGCGCTTGATGGCTTGATCAAGCAACTTAAAGTTTTGATCCATAAATATCTTTTTTTTAAAAACTGTTAATTCCTGCAATTAACTGCTCTTCAATCTGATCAATCTCTTTAAAAGGATGTTTTTTGTGTTTTAAGCTCAAACGGGAATGAACATAGTTGAAAGGACTGAGTCAACCATTGATTAAACCAAAGCAAAACATTGGTAAAAAAGCAGTTGCAACATCAAATAAACCAACAATAGCAATTAGATAAAAGATCACAAGGTTAAAGTTGCCATCAATCCCACCAAAGTTAAAACCACTGTAGATAATAAAAATGAAAACTAAGCTAGAACTTTCAATCAGATCTATCTTCCACTGCGAGTTGAAGTTATCGTTAAATAAGTTTTTGATAAATACCTTGTCCTTTAAGCTCCTTTTAAACCAGTAAAAGTAAGTCCAAGTTGTTGTTTGTGATACCTTAGCTGCAAAAAAGAGCCAAAACAGATAAACAAAGGTAATTAACTGGATTGTTGATTGGTCTATACTAAACAGATAGTTAGTTAGATACATCACTGCAAACAAACCACTACATAACAAGATAAAGCTAACCAAATAACTTAAACAATAAGCAACACCGTATCTAATCAAACAATAAGCTAAGTACAGTAAACTAACTATTCCTATCCCAGCAATAATCCCGTTACTTGATTCAACATCTGCTGCTAAAAATTCTCCTAACAGGTTAGGTTGAACAAGATATAAAACTAGCATTACAACTCCTATAGCTAACAAGATTAGCCATACAAACAAAAACTTCCAGTTGTTTTTCAAAAAGAAATCATGTTGGTTTTTGGTTATAAAAAAGTCTGTGTTCAAGTCACTAGTTGAACTTAAAAGTGAATAGTTACTAGAAGTTTTAGTTAGTGCTTTTGCATCCTTCTGATAGAGAAAAAGCTTTCAGTTTGCACCTGATTCTGAACTGACAAACAGTGAGATTAAAACGATGCTGATTCCATAACTAAAAAAGTAAGAAGTAATGGCTGATATTGCCATCAAATTAGCAAGTTGTTGGACCTGATAATTAGAAAGATAGATAACAACTAAAGCACTAATTAACCAGGTAATGTGAAACTCAAGATTAGCAAAAAAGCTTCTTTTTAAACACAGTTTTCACGATTCGATAATAGAAGCATTATTTCTAATATTTCTTAAGAATAACTCTCCTAAACTCAAGAGATTAATTAGGTTGATGGCAATAACAAAGAAGATGCCCACAAAAGAAAAGACATCAACAACTCCACCAACTGCACTAAAGATAACCAGTGATGACACCACACTTAACCCTAAAGCTAATGCTTTGTATAACCCTAAGAGCTTGTATCGTAGTGTTAATAGAACAGCAGCAATAAGAACGATGATACCAAACGCAATAAAACTAGAAGCAAAATTAGAAACATCTGTGATGGTATTTGCAAGTGGAGCGTTAACCAGACTTGTTTTTGCAGTTGCAATGGTAGCTGCTGTGGGAAATGGATTTGTTAAAAGCTCTTTAACAGTAGCTGCTGTTACAAAGTTTGGTGGGGTGTATGTACCATCACTACTCAAGAAATTACCAACTTGAAACTTAATATTACCACCATATTCTATCCGTTGGTTGTTAGTGTCTAAAAAGCTTTGTAGAGTAGGATTAATCTTAACAGGATCACCGGTTTTGCTAACCGGATCTTCAGTTCTAATTTCACTGATAATATAGTTATGGAATAACCCTGAAAAACCACTGCTATCTGCACTCCTTAACTTTCTTGTATCAGCACTATTTGAAACTGTAATTACATCTGAACTATTGTTGTTATTGTTATTATTCTGGTTATTATTGTCATTACTGTTGCTAGCAACCGCTGAAAAGTGGTTAGGAGCAGCGTAGATATAATAAAGGTTTTTGAGAGTAATGTCACTTGCTTTTTTAATAGGAGATGCCCCATTAGCATCAGTGTTTAAAAACAGATTATCACCACTAGCAAAGCTACCCTTTGCATGTAAAAATTCCCAGAGACTCTTCTCTCTTTCATTAAAGGTGAGATAATCACTACTACCCTCAACTACATTAAAGATATTACGGACATAGTTTAATGCGCCTTGTTTATCTTTTCACAATACTAAAGTTTTATTACCACTACTTGAAGTTCCATTCGCATTTTGCTTAAAAAATTTACCATTGCCCTCAACACTAGCTTGCCCATTACCAGTATTATAATCAGAACTTGATAAGTTTTTAGAGTTGATAAAATCAAGAAAATCGGTGTGTAAACTTTCTTTGGTAACTACATTTTTATTTAACTTGTATTCAAAGGTTAAAGCACTGTTTACAGAAGTGTTTTGGGGAACAACTGCTCTAATGGAAGCATTAATAAAATTATTATCCAATGAATCAAATAAAACCACTCCATCAGTTGTTTCAAGTGAAAGTTGGTAGTTGTGTTCAACTGAACTAATTGCATTTCTTCTAACCTGAGCATCAACTTGTCTTTTTCTTTGGCGGGTTTTGGCTTGCTCACGGTTTTGTTCAAAGGTGAGAGTAACAGAAGGTAATCCCCCATTAACCAATCAGGACTTGTCAATGTTGTCAAGTTCACTAGCAGTTTTATCATTAGTGTTTTTAGTAATGTTTACAGAAGAAAAGCCCTGAATGAATAAACTGTTAGCATAACTTTTTTCAACACCATCTAAAAAGCTATCAATGTTAGTGATGTTTTCAATCCCGTTGGTTGGTTTTGTTTGCTGAGGATCTAATGAGGTATTGTTAGTTGACTTATGGTTTAAAAAGTAAACAGTTGTAGTGGTAGAACCGTTGAAAACAGCACCTAACCTGCTGTCATTTAACAGTTTGTAACTCCCAAAAATAACACCAAACAAACCCAGACAAACAAGTCCAAGAATAGTTCCTATTTTTAGGATTCAGTCCAAAGAAAAGCGCTTTTTGAACCTCACTAACTATTTTTAATAACTTAAATTTTATATAAATATTAAGTAATGGCAACCATTCAGGAAATTGAGTGTGATTTTTTAGCTAAAATAGCACAAAAATTTACTAATGCAGAGATTGAATTAATTAACAAAGCATTCTATCACGCTAAAACTTGGCATGAAAACCAGAAACGGCTTAGCGGTGAACCTTTTTTTATCCATCCTTTAAGAACGGCATTATCACTAGTTGAATGGAACATGGATCCTATCACTATTTGTGCTGGTTTGTTACATGACATCATTGAAGATACAGACCAAACCGAAGCTAATATAGCAATGATTTTTAACAAAGAAATTGCTGAGCTTGTCACTAAGGTTACAAAGATTACCAATGAATCTAAAAAGCAACGTCATCTCAAAAATAAAAAGGAGAATCTTAACTTAAAAAGCTTTGTTAACATTGCAATCAATTCTCAACAAGAGATAAATGTAATGGTACTAAAACTAGCAGATCGACTTGATAACATCGCTTCCATTGAGTTTCTCCCCATTGAAAAGCAAAAGGTAATTGCAAAAGAAACTTTAGAACTTTATGCAAAGATTGCTGGGAGGATTGGGATGTATCCTGTTAAAACAAAATTAGCAGATCTTTCATTTAAGGTGTTGGATTTAAAAAACTATGATAACACCCTGTCAAAGATTAACAAGCAAAAGGTCTTTTATGACAATGAGTGGGATAACTTCAAACAACAATTAAAAAAAATCTTAGCGCAAAATCAGATAGAATACCAACTTGAAAGTCGGATTAAAGGCATTTACTCTACATATAAAAAACTAACTGTTCATGAACAGAACATCAGTAAGATCCATGATCTTTTTGCTATCCGCTTAATTACTAAATCAGAACTTGATTGTTATCACATCCTTGGTTTAATTCACCTTAATTTTTTAATTGACAGTAAATACTTCAAAGACTATATTGCCTCACCTAAACAAAACCTTTACCAATCAATTCATACCACTGTTCGTTTAAAAGGGTTAAATGTTGAGATCCAAATTAGAACCCAACAGATGGACAATGTTAGTAAGTTTGGCTTAGCTAGTCACTGGATCTACAAAGAACAGAAAGAGGGATTGTTAGCACCTGCTTTGCAACTTAATTACCTAGTGACAAAACAAAAACACTCACATGATTTTCTAAAAAGGATTTTTGGGACTGATATTATCAAGATTAATGTTAGTGCTAGTCATGAACCTAATGTAATTAAGCAAATTAATGTTGATAGCAACAATAAACTCCTTGATATTGCTTTTGAAAACTATCCCAAGCAATTTGCTAAATTAACCAAAATTGAAATTGATGGGGTTGAGATCAATTCTTTTGATACTAGTGTTGAAAATGAGATGCTGATTGAATTTTACTTTGGCAAGAATAACAATTTGAAATCAAAGTGAATTAGGTATATGAATAACCCTATATACCGTGAAAAGGTAAAAAAGAGCTTGGCTAAACTAGCTAAATCTGGTAGATACAGTGAGTTAGCTTCTTATGAAAAAGAACTGGGTGAAAAACAGTTAAAACTTGCTAGTGAAACTGAAATCCAAAAACGCTTAAACACCCTAAGAATTAAAAAAATGAGTGATTACTTAGCGTTAATTGAGTGTACTAACTTTACTAATGATGAACATTTGTTGTTTCTAGCTAAAAACAACGACAAGTGAAATAAACTAACAAAACCACTTAAGTTTGCTTTTTCAAAAGTAGTTTTTCACAACTCTTACTTTGAACAAATTGAAGGTATTTTTATCACCAAAATAGTGATTGAACCATGTTGTAGTAAGATCCCTGATATGCCTGAACAAGTAACTGGTATCTTAACTAAAAACATTTTAAGTGTTCACCGTTATGGTTGTAAGAATTTACAAAATAAAAAGCAGTTAAAAATTATCCCGTTATATTGAAATATCCAGCAGTTAAAACTAAAACCACGTAAGTTTCGCAGTTACATTAACATTAACGGAGTGTGGAGTGAAAAAACCATTAATAAAATCTGTCAAACAATTATTAATGGTGATGGTTATATTGAAAAAATAATTCCCAAGATCAACAAACAAAAAGATGAATTTGATTTAAACATCACCCTTTTTGTTAATAACTACCAACAACTTCTCACCTTAATGGACCAAATTACCACTAAGAATATCAGCTTTAGTTGAAAATACCTTTAGTTACCAAACACCGCATCATACACTTCTTCATCTCTTAAAAGTAAAACTAAGTGCTTTTTTAGTAGCCGTTGTGGATTGAAACGTGATACTCTCACTCCATAAACTTTCTTTGTAAATAAAATTAATAAAAAACCAGAAACAAAAAATGCCCCAATAATTGAGATTCCTGTATAGAAAAGGATTTGGGAAACTTCAGTTAAATACCGCTTAAAATCATCATTTTGGTTTGGATTTGGGAACTTTTCTAGCATTTGATCAACAAAGCTAGTTATGTTATTTAATGTGTGTTTAATTTGGGTAAAAGCAGCATTACTATCAAAGTTATTAATTTTTTTCAATGAACCTTCAAGTGCATTTAAAGAACTATCTAACCCTGATGCAAATGAATTGTTTTGCGTTAATTTTCGCCCTTCGTTTAAAGATTTTTCAACTTCTGAAAGTGTTTTAACTAGGTTATTTTGGAAGATATTTTGGGAGATCTTTTTAATTTCAGTGTTATATTCACTAATCTTGTTATATCCCTCTTTATAGTAGTTGTTAATAGGTTCACGGTAAGTAAAAGCAAAATAACCAGCAGTAGCAATTCCACCTAACAATAAAATTCCTACCAAAATAATAAAAAAACTGCAAGTTTTTTTTAATAGTCTAATCATGGTCTAATGCTGTTATCACTTCCTCTTCCAAATCAGGATAACGATCTAAGATATCATTAACATGATCAGCTAACTGTTCAGTACTAGTAAATCTTGAAAACCTGATTAATCCCCTTTTTTTAGAAGTTAAAAAGGAAAAGACAATGGTTGTAATCAAAATTGCTAGCATCCCACCTGATACAGCAACAATGGTTATCATTGTGACATCATAATAATTATTGAACTGTTCAGAAGAAGGGATGTTGTTTTTGATCTTTTCATAGCTACTAACAATGCTTTGTGCTTGTGTAGAAAACTCACTAACTGCACTAATTACACCATCAGTGGTTGCTTTTTTTTTTACAGATGGAGATGAACTAACAGTACCATTTTTATCAAGATTACTTTTAAAGTTTTCTAATTGTGTTTTACTGTTTTCCAAGTCTTTTTTGGTTGCATCAAGTTCTCTAATCTTTTCTTGACTAATATTACCTAGGTTGTTCTGACTCTCTAAGTTCTTTTTGTATTCATCTACTTTTTGTAAAGATTGCTCTAAGTCTTTTTTAACTTGATCTAAATTGTTAGTAATGGAACTTTTGCTAAATTGGTTAGCACTATTTGCTAAAGCATTAGCAAAGGCTTTAGCAGAATTGAAAGAACTATCAATGGAATTACGAGCTTGAACATAAAGGTTAGCTGCTGTACTACGAAAATACAAAGCAGTAATACCAAACCCTAACAGAAATACAAAAACCAACATTCCAAAGAAAAGCAAGATGGTTTTTGAAAACCGATTAATAAAAATAACCATTTAAATACTTGTACTTTTAACCAAACTAATGGTTATAAAAGTGTTTTGAAAACAAAACTACTAGAGTTTAAGTTTAACACCCAATGCGTTGGTTTTAAGTTCTTTTGCTAGTGATTTAACTTCATTTGTATCACTGGAAACACCAGTAATATCAACTGTTTCAAACTTTTCTTCACCAATTACAGTAACCAACCCTTCAACTGCTTTAGTCATTCTTTCAAGACTACTATCTGTATCATTTTCTAGTTTTTGGAACTGTACACTGTATTTACCACTAGCAGTAATTCTTTTGATGTTTTCATCAAATGTTACTCCGTTTTGTTCAGCACGCTTTAAAATTTCATTTTGAAACTCGCTGTCTCTGCTTGCACGTTCATTTTCATAGATGCTAGCACCAGGGTAATAGTTTTCATTAACCCTGTAATAGGTGTTATCTCCTTCTCTGTAAGCTTCTTCTAAGTGGAGGTTAAGTTCTTTTAAAGAACGGTACACCAAATCATCATCACTATCCTTGTTGGTATTAACATTCTTAACTAAGTACTTGTCAATGTATCCCCCAGCAAAATAACCTTGGAACTGTCTTTCAAACCTTCTGTAGTTGTAAATATCACCAACTGCTTGTTTGAGCTTATTAGCATCAATGGCTGAATTATCACTGTTTTGGAGTGTAACTTGGGTTAAATCAATGTGGGTAAATGGTTTTGAGGCAAACAGATCATAGATAACATTAGTTTTACTTCCCAATACTAATGGGTTAAAACCAAAACTATTTGCTTTACTGGTGGAATAAAATTCCAACTCAACTACGTTTGCAAAGATTTGTTTAGCTACATTAACACCAGTGTAATCACCATAAAGTGAAACTTTTTTAACTGATTCTGGTAATTGCAATTCATTAAGTTGTGAACCATTACCACTTAAACCTCTAATAATTAGGTAAGTAGGTTTTTCTGAATCTATTGTTGTTTTTAAAGCACTGATATCTTTAGCATCAATTGCGAGTGTTTTAACATCTTCAAAGAAGTGGTTTGGGTATGAGTCATAAAGTGGTGAACCTTTAGCAGCAAAATAAGTTCACTTATCATAAGTAAGGTTATTACTAGTTGAAACAGTTGTTGCTTTTGTGTTACTCCATCCGCTGAAACTAGGAACTTGTCTAAACACATCATCATAACCACGGTTGTTTGCCATGAGAGTACCGTTAGTTGTTTGCAAGATTGGCATCAACACGCGGTTATTGAAATATTCAGTTGATTGGTAATAGCTAAGCAGTGAACCATCACTAGCAACATGATCATAATAACCAGCTTTAAAACTAGGAGTAATAAAGTTATCACCTGTTTTAATTAAGCCACTAATTTCAGTTTTAGGAACAGGACGGTCTAAACCCTTTGGACTGTTAGTGATCGCTTCTGATAACTCATTAGCAAAGTTTCTAAATTTCTCACGGAAGTTAGCACCACCAGATAAATCAATCTCACTCTGATAGCTACCATCGTTTAAAGAAAGTGATTTCAACTTTACTTCTGCACTAGGAGATTGTGTTGAGAACAACTTAAAACCGAGGTTACCACTCCTACCTGGTACAACCAAAGCATAACCACTTTGATTAACTAGGTTAGTGATCATAACTGCTGAGAATGTGGTTAACGCTCCAGCACCTAAAACACCAATAGTCCAAAATAATTTACGCTTAAATTTTACACTATTTTTATGTTTTTTAAATTGCATTATTTTTTGTATTATTAGAAACTTTTAATTTGTAAACAGTAGTCTACTTATCAATTTTAAATTAAATCCACTAGTAAACTTCAATGACCCTTTCTCTGAAAGAGCTTGGTAATTTATTAAACAAATAAAAAACACCCTTAAATTGCTTTAAGGATGTAATGAGTTTAAATCTTTAATTTTTGGAGCGAGTAATCGGAATCGAACCGACACGATCAGCTTGGAAGGCTGATGTTCTACCATTAAACTATACTCGCACAAATTTGGTGCCCAAAGCCGGACTTGAACCGGCACACCATTACTGGCGTGGGATCTTAAATCCCATGCGTCTACCATTCCGCCACTTGGGCTGGTGACCCACCCGAGGATCGAACTCGGGACCCTTTGATTAAAAGTCAAATGCTCTACCGCTGAGCTAGTGAGTCTTTTTGATAGGAATTTAACCCTATCTTTAGAAATTTAAGCTTTAACTGGCTGGGATGGATGGGATCGAACCATCGCATGATAGAGTCAAAGTCTATTGCCTTACCGCTTGGCTACATCCCAAAAATGGTGGACAGGGAGAGATTCGAACTCCCGAAACCATAAAGGTGTCTGATCTACAGTCAGAAGCGTTTAGCCACTTCGCTACCTGTCCATTACTACCAGCAAGATATTTTACAAATTATCTGTTGCTGATCTTCAGGATTTTAACACGATAGGGAACTTCAATACCAACAACATCACATTCATCACCAATTAAACGCCCATAGATTGCAAGGGCAAGGGGTGATTCATTGGAAATTTTGTGTTCTTCAGGATTTGCTTCAAGTGTACCTACAATTGTGTATTTTTCGTTGGATTTAGAACTGTAATCATAGATCTCAACAGTGCTCCCTAAGCTAACTTTTGTTACTTTGGTTTTTGCTTGGTGGTCACTAATTAACTTAGCGTTGGCTAATATATCTTGAATTTCAGCAATCCTAGTTTCAATCTCACCTTGCTGTGCTTTAGCTGCATCATAATCAGCATTTTCACTTAAATCACCCTGATCACGTGCTTCTTGTAAGAGTCTGATAATCTCAGGACGTTTAACTTGGATTAGGTTTTCAAGTTCTTTTTCCAGTTGCTTAAATCCTTCTTGAGTTAGGTAATTTTTATTTAGTTCCATGACAAAATTAAAAGCAGCAAAAACACTTTTATTATTTAATTATTTCCCCTTTTTTTGCCAATTTTTGCTAAAAAAATAGAAAATCAGTATTGCAGTAATATAGATATTAAATAATAAATTTCTATCTAACTTCGAAATAATAAACTCAAGATCAACAGAAGATTGTGAGTTTTAATACAGATGAGAGAAAACAATAGTAATGCTCAAAACAAAAAGAAGATCCGCTTTTTAGCTTTGGGCATCATTATTCTAATCGTACTAATTGCTTTAATTACTATCCTTTTTATCACTGGAGTAATTAGTCGTAACCGTTAAGATTACTTTTTGCTAGATGACAAAAAAAACACAAGATCTCACTAGTTGGTATGACCAACTGCTAGTTAAAGCAAAGTTAATTTGTCATGGTGAAGTTAAAGGTACAGTTTGTTTTTTAAATAACAGTTGAGGCTTATGGATGGAAATCCAACAGCTTTACAATGATGCAATTGCAAATAAAAATCAATTGTCTGCAATTGCTCTAACTAAATTCCAACCAACTACTAGTTTTTGTTATCAAGTATTCCAAGTACAACTCCCTACCCTTTCTTTTTACAGTGAATATCAAAAGGAAAAAACCCATATCAAAGGTTTTAATCCTGAGCTTTTTTTAATTAATCAAGTTGGTCAAAAACAACTCAATGATCCTTTGGTTTTACGACCTACTAGTGAGATTGCTTTTTGCAACTTATGGAAAAAACAAGAGTTATCTTACCATGATCTACCTTTAATTTATAACCAGTGAACTCAGGTTTTTCGTGCAGAAAAAAACACCAGACCTTTTTTGAGAAACAGTGAGTTTTACTGACAAGAAACTCATGGGCTTTTTGTGAATCAGAGCCAATCTGAACAAGCTGCTATTAGCTTTTGAAATTTATATCAGGATTTAATTATTAACAAACTTTGTATCCCTGCTTTTGTTGGTTTGAAAAGTGAAAGTGAAAAATTTGCAGGTGCTAAAAACACATGGACAATAGAAGCAATTATGCCTGATGGACAAAGTTTGCAATGTGCCACTAGCCATGATTTAAGTGACACTTTTACAAAGAGTTTTACTATCAGCTATCAGAGTAAAACTAACCAAAAAATGACTCCAAGTAGTTTTAGTTGTGGGATGTCAACTAGGATCTTAGGAGCAATTTTTTTAACCCACAGCGATGATTATGGTTTGGTTTTACCTTGGTATCTAGCAAGTAAACAAGTCAAGTTATACCTGTTTGATAAAAACAATAACCCTAAAACAAGAGCTTTAGCTTTTTTAGTGAAGGATTTTTTAGAAAAACTCAAAATTCGCTTTAGTTTTATAGAAATTAACAATCAACTAGGTAAACAACTTTTAAAAGGAGAAATAGAAGGTATTCCACTACAGATGATTGTTGATAATGAAAAAACTATTAACATCTTCAACCGCTTAACACGTTTAAAAACCAGCTTAACATTTGCAAATCTTCAAACTGAATTTGTTAATTTAGTTGAAAACTACCATACAGAGATGTATAGAAAAGCAAATGATTTAGTTGAACAAAAACTAGCAAGAGTACAAACTTTAAAGGAAATTGAACAAGCATTCAAAAATAAAAAGGCTGTTTTATGTACCGTTAAGTTAACTGGTGAACTTGAACAACACTTAAAGACAAAATACCAAGTTAGTGTTAGGTGTTTTTTTAAAAAGTCAGATGTAACACAAAACTGTCCTTTTACAAATCAACCTTGTTTTGATTCAGTTTTAATTGCACGTGCTTACTAACAAAGTACTGCCAATCACTACCCAATAAAACCCTTGATTATTTGGTTTTATTTAATTTATTTCTAAATATTTTTTACCAGTAATTTTTGTTTAAAAACAAACTCGTTTTGTAAAAGAAAAAAGTTAGAATTGTTGTATTTCAATAACGATTATTTAGCCTCTAAAATCGATTAGACGATCTTCTGATCGTTTAAGATTTATTCAAATTCAACATGACTTTATGACTTTTACAAACAAAGAAAAAAATTGCAAACAAAGCAAATCATTAGCAGCGTTGATGACAAAATTTAAGCGCTCGCAATTAATCTTGAAACACCAAGCTAATAACATAGCACTGGAGTTGTGAAATGAAAATGATATTAACCTATCAAAACAGCTTATTGAGTTAATAGAAGATACTTTTTCAATGCTTAAAAAAGAAACTGTTGATTTTATCTATGACATCTATATTTATGGCAAAAAGCCGTGTGATATTGGTTATTCTAATTCCACCTATTACAAAAAACTAAACAAAGCAGCTAATAGTTTTTTTGACCATTTTGTTTGAGATTCGTCAATATTAGACAAAAGGATCATAACTAATGGCAGCAATTCTCAACGTACAACAAATTCAAAATAACCAAGTTACTGAGTACACAATGAGTCCAGTGCGTAATTTTGCAAACACAAAAGATGTATATTTTGATGCGCAATTAACAAATATAGAAAGCAAAATTGATAGTAGTAGGGCTCAAATTCACCTTACTATTGCACTAAAATACAACACTAATCTCCCTGATAATATCTTTCAAGCCCACTTCAGTTTAGGCAATTGACAAAGTGATAAGATCCAACTTCAAAAAGCTCCTGATAAAAAACACGATAGTTTAAACAACATTAAGTATTTTTATGCATTTCTAGATGTACCCCGTTCAGCACTGGCAAAAAAAGAAATTAATAGGTTTAGTGATGTGGTTGCTAGAGTATTGAGAATAAGTTTTCGCTTGCAAGATCAATCTGAAAAAGGAAATTGAAGTGACTATCATTTGTTTGATACTGTAGCTAGTGAATTGTATGCAACAGTAATTAAAGAAACAATCAACTTTGGCAACATGATTAAGATTAATGTACTTGATGGTAGTAAACAGCTAACTAGTAGCCAAGGAAGTTTTAAATACAGTTGAACAATGTATGACTATAGGAATTTAGAACAACTTGATGAGGTGAGAAACTTGATCAATATCAGTTTTGACAAACCAGTTCAAATAGTAAATGTTGATGTCAAAATTCACTATGTTCCAACTAAAGGTAGGTTACAAGAGATAAAACAACAAGGTGAGTTTGAAAATAACCTTGATGTTAATGAAAAGCTTAAACTCAATTTAATAGGCAATTGAAATTTTGACAAAAATAACAAGAAGCTAATCAGTGATATTTCAGGTACTGAAATCTTTTTACCTCAGGGTGGTTATGAGAGTTATGAAATAATGATTGGCGTTACAGTAGGTAATGATTTCTACACAATTATAGCCAACAACCAGTTTAAGTACGAAACACCTTTAGATGATCTTGAACAAAATGACTTTTTTGAGGTAAATTATTTACCTGTTTACAGTACATACAACTTTAGTGATTTAACCCAGTAAGTATGATTTTTTCAATTAGTAAACGAAAGTTAATTTGCGGATTTTTGCTAGTTATTCTAACAATAGGTGGGGTTTTGGGTGGTGTTTATTTAGTTACTAAAAACAACAAGGATAACTACCAAAATGAAAGTAATTTCAACAATCAAGAACAAATTAGTAAAATCCCTAATTTCAAAGCTATTGGTCCTGAAACACAACGTATCTTAAGAGAAAGAAACTATCCTTTAGATGATAGTGGTTATTATGTTTATAAGTATGGTGAAATTAATAGGTATCTAAGAAATGAAAGTGAACTAGATGAATTGATTAACTATAGAGTGATGGTACCTTCACTTAAATCACATCACAAAAGGGTTAATTTTGATAAGGCATTTCTTGAAAGTAAATTAAGAAAATGGATTATCAAAGCAATTAAGCAACATAACTATTTCCAACACTTTGAAAATGAACCAAATTTAAGAGTTCAATATAACATGAATATTCCAGCACAAAAAATTGATGTTAATGCTGTTTGATCTTATAAAAAAGATAATGATGCTGCTACTGGTAAGCCAATCCGATATTGAGATCAATTTGAGCTTAAACTTAAATAATAAATCTTTATAATTTTTACTAGATTTACTAATGCAAACGCATGAAATTCTTTTAAAAATTAAAGAAATTGCTAAATCAAAAAACTTTAATCTTAATTTAGATGAAAAGACAATAAATCAACCACTTCGTGAGTTGAAAATCGATTCACTTGATATGTTTAGTATTGTTGTTAGTCTAGAAAATGAATTTGGGATTAGTTTTGATGATGAAAAGTTAATGAATCTAAAAAATCTTGCTGACTTGGTTTTAGAAGTTAAAAACCTTTTAGCAAAAAAGGGGTATAGTTCAAAGGTAGAACATCTGTCTTCAAAATAGAGTGTTGTGGGTTCGAGTCCTGCTACCCCTGCCATAAAATATAAAAGCTTAGTAAGTCTCTACTAAGCTTTTTTCAATCCCCCCATTCCCCCAACCTCTATTTTGTGTTACTAGTGCCTAAGGTGGTAGTGGAGTATCACCAACTTGATAAGAAGGTTGTCAAAGAGAGTTTGGAAGTGGAAGCAACTGATTCCTTCAACCCCACCCAAAGGTTGCAAAAAGATAGTCCAATGAAGGATTCAACAAAAACAGGGGAGAAACTCCAAGAGACCATGTCATCAATGAGTGGTGATGGGGCTACATCCACTCGCAAGGCCCTCAAAGTGGAGGTGGAAAGGGGGAGTAAAGTCAATCAAGGCGAACTTTTAAAAAACGACTTTGCTAAAAAGCCACTGAAACATAAAGAAAATAGTGGGGAAGTGAAGTTAGATGCGAGTGGGGAGTTTACTGAGGCCTGAAAACCGGTGTTGACTACCGAACAAATAAAAGATAATAGGGGGATGGGGGCGACTTAGACTTTCTCCCCTGAATCGGCAACAACAAACCCTTCTCCAACTCCCACACTGCTACCCTTTCTGCTTCTTCTTCTACCCCACTCCCCACTTTTTCTAACATCAATGTCGGGGTTAAATCAATGATCACTCATCTAAACAAAGAAAACACCCGGTGGGTGTTTACCCCTAACTCTTCACCAGACATTTGAACGGGAGCAGGGTATCGCAAGGATCATCAAACACCAACGGCATCTCCTTGACAAGTGTGTTGCCTAGTAGTAGTAGTAGTACTTCCGTTTAATCCCTCCTCTGCTGAAAATCAAGTCACCCCAGCTGGTGGCTCCTCTCCAAAAAACCAACCACCTACACCCATTTACCCAACAGTATCAGTCCCACCAGTGACTGGATCAATGCGTTGACTTTCACCAATAAGAATAATCCGCAGCGCAATCAGTTGTTGCTGAGAGCACTGTTAGGAACTATCCCGGTATTGATCAATAAGAGTGGGGAGGGTGGGGAAGAGTTTACCCATACGAGTGAGCAGCAGTGAAACGAAACAGAAAAACCTAATGGCAACCTCCCAGGGTTTGGGGAGGTGAATGGTGGTTTTTATCAACTAAATAAAAACTTATTAGCTTATTTTTATTAGGTTTTTACTTATTTAATAGTTAAAAAAGTTTTGAATTTTTCTTAGTTTTTTATTTGTTTAATAGTTAAAAAACACTAGGCTTTACCTTTATTTAATTAATAAAACCTTTACCCCTATTACCAAACCATCCATAACACCAACTTGTTTGTGTTGTTCAAGTCTACTCAGGTGAAAGTTAAGTATGACAGTTCAAGTGGCAGTAACAACCAGATTAGTTTTGATTCAACTAACAACAAACCATCCTACATCGTTGAGTTTACTAACTCTACCAACATTGGCATCAAGTGAAGCGTGGTGAAAAAATATCAGTTAGATGTACCGAATGTTACCAATGAGATGAACGATGTACTGAAAGAGTTGATCCTTGAACAACCTTTGACTAAGTATACCTTAAATTCGAGTTTGGCCAAAGAAAAGGGCAAAACGCAAGCAGTGGTGCATCTGGGTAGTGGTAAAGCAAATCAGTGACAATCGATGCGTAATCAACACAACTTAGACAACAATCCCAGTCCCAATGCATCAACCGGATTTAAACTCACTACCGGCAATGCATATAGAAAATTAGATCAATCCTGACCAATTTATCAACCAATTGATGGGACCAAGCAGGGCAAAGGGAAGGATAGTAGTGGGTGGAGTTCAACAGAAGCAACAATGGCAAAAAATGATGCGCCCAGTGTTACTGGAGGGGGATCAGATACTTCTTCAAAATTTAAAAGATACCTCAACACCAAACAAGCATTGGAAAGCATCGGCATCTTGTTTGATGGGGATGGAATGAGGAATGTTATCACCCAGCTCTATTATGCTTCTACCAGTAAGCTAGCAGTCACTAACAACCACATTGTCGTGATGGGTAACAGCTTTCTACCCAGCTTGTGGTACTGGGTGGTGGATCGGGGTGCAACAACTGATTCATCATCAAAACCCACCTGGTTTGCTAACACCAATTTAGATTGAGGAGAAGACAAACAAAAACAATTTGTAGAGAACCAGTTGGGGTATAAGGAAACTACCAGTACCAATTCCCACAACTTCCATTCCAAATCTTTCACCCAACCTGCATATCTGATCAGTGGCATTGACAGTGTCAATGATCAGTTGATCTTCAGTGGTTTTAAAGCAGGGAGTGTGGGGTATGATAGTAGTAGTAGTAGTAGTACTACAAACCAAAGACCAAGCACTTGCTTGATCAACAACAACTAGCTTAGATAGTAAAACGGGGTATAAGGATTTGGTGACCAACGACACGGGTTTAAATGGTCCAATCAATGGGAGTTTTTCAATCCAAGACACCTTCAGCTTTGTTGTTCCTTATTCGGGGAATCATAACAATAATGGAACAACTGGACCCATTAAAACTGCTTATCCAGTGAAAAAAAGTGAAGCTTCAACAGTAATGATCAATTCCTTGATTAACGCTACGCCGTTGAATAGTTATGGGGATGAGGGGATTGGGGTTAGAAATATTTTTATGAGTTTAAAACAAGGTAATGCAGATAACTAGCTAAATCAACAACTGGAGGTTTTGTACCCCCATTTCTTAAATCTAAATTATGTAAGTTTTGCTGTTTAAGTAAATCCATAAAATTTTTATCTTGAACTTTTATCTGCTCTTTTACGTGTAAAGTGAAAAGTGGCTGATTGTCTTTAAATTGAAATAAAGAACCTGGATAATCAGTATTTTGAACTAAATAGCTATCCAAATCACCATCAACGATAAATTTAACATCCATTTCACCTTTTCATGAAGATGGAATACCTATTCATGGACCAATAAATTTAGATCAAAAGTTTAATTCATAACCACCCTGGTATTTTATGTCAATTTCAAATTTAAATTTGTATGGATATTCTTGTCTAAAATCAATTCCACCTTCTTTTCATGAAATTGTTGGATTATTTTCCTTGTTTTCAACATCAACCTTATTTAAATTCAAGCTTATGTTAGTATGTGACCTCAATTTATCTCTTAATCAGTCCGACATATAAAGACCTTCAACTCCTTGTTTAGGAAACTCTATCCCCTTTTCACCAAAAGCTTTTTTAGCGTTTTGATTTTCATCTTTTTTGTTGTTTTTGTAATACTCCTTTATTGATTTAGTAAAGCTGTCAATTAAAAATTTGAAGGTCTTATTTTTATCAGCAGCAAAAGAAGTTTTTAGAGCATCTATTAGTTGAGTCTTATCAGTAACATTTTTGCCTTTTGTAGATCAATAATTTTTAAATTCAGTTGCAGTTGAAAGCGACTTATCAAATTTTTCTTGTTCAGCTTTTAAGCGCTTAGCTTCCTCTTCTTTTCGTTTAAGTTCTTCTTCTTGTTTTCTTCTTTCCTCAGCTAAAATTCGTTGTTGTTCTTCATAATCAGCTTTAGCTTTTTCACGTTCTTTTTTAAATGGTTCAATTATTTCTTTTTCAAACTTTGTTTTAGCTAAATCTAGTTGTTCATCAATTTTTAAAACTGAATTAAAGATATTTGTATTGGAAAGAAAAAGTGAATCACTTACAGCAGTTTTAGCGATTGAAGTTAGATCATTTTGATTTTTATCAACAAGTTTATTAATTAGAGGTTTTCAAAACAATTGAGTATTTATATCGTTACCATTAAAACTATTTTGCAACTCTTGTAAATTCAAAAAATTAGAAGTTTGGGCTTTTAAATCAAATTGCAAGTTAACAGCGTTGTTACCACCTTCATAATCAACTTCAAAATCCATTTTGATTTCAAAGCTATAACGAATCTTATCTTTAATAACAATGTCATTTTTTTTAACCAATTCAAGATTAAAGTCTTTTAATTGAATTTTCTTTAAGTTGTATTGATTAAATATTTCAACAATACTTTCAGTAATGTTTAAACCTTTTGGATGCAATTGTGGAAAGAAATTTCCATTAAGGAACTTAAAAAAATTCTGTGGAACTTTAGGCAATTTATCTTCATCATCATATTGGAAAATTTTGTTAGTTTCATTAATGCTTGATTGCAGCCTATTTAATAAATCAACTGTTTTTTTAATACCTGATTTAAAACCACTTAAAAGATGAAAAATATCTATAACATTAAAGTTCCTAGGATCTAAAACTTCATTCTTAAATTTCTCTCGAAATAAGTCAAAGTTTTTATCATAATCTTTCTCTTTAGCAATATAGTTTTCTTCTTGCTCTGTTATAGCTGTTTGCTGTTGGAGTAGAATACTATTTTCTCTACTATTTGTTGTACTTGATTTTCTTAGACTAGGTTGACTTAAATTTGCAGCTGAGCCATTATAGATAGAAGGGACAATAAGTACACCAGCAGTTAAAGTTGATGCAAAAGAGACAAAGAGAAAAGTTTTAATTTTAAAACGAGATTTTTTGATAGCTTTCAAAGTTAATAATTAATATTGAAAATAATTAGCTAAAACTAAAAAAGTTACAAACGAAATTGAATTTGTTTAAAACTAAAACAGACAGGGAAAATTTAACATTATATTCTAACTCTGAATAGCAAAAAATATAAGAATAGCTTTAATTCAAAAAAGTAATTCGGATCTTAAAAAATATTTTTTAATAAAGATTAATTGCCAGCAACTTGAATCTAAGTAATAAAGTGAATATTTATTTTTATTAAAAACTTATATTCAAATTTGTAGTCTATTTTTAACTAATATAAAAAAGTTGAATATTTAAAAATAATTAATTTTGATAATTAATAATCCAGGATTAATAAGTGCTTTTCAAAAAATTTACTTGGGTCATTCCAAGTTTGTTTTTAACAATTATTTCAACATCTTTACTTATTAGTTGTGCAACTAAAAGCGATAACACCTTAATATTCAATATTTCACTTGATCATAACGCTGATACATCAATAGAAAAATTCTTTACTGTTTTTTCAAAAAAACTTAGTGAAAAACTGAATAAAAAAATTAATGTTAACTTTAATATAGTTGATGATTCCTTTACAAAAATTAACAATATTCAAGCTAATAAGGCAGATTTTGCTTTTGTTAATTCGCAAGCTATTGCTTCAAATAATTGGTTTGGCTATACGCCATTAATACAAACTTTAACAACAGCTTTTAAAGAAGATTTGGAGCTTGATTATTATGAAGATGGTAATTTACAAAAAAAAGCTGAAAAAACGAATTTACTTTTTCTAAGTCCACCTTACAAAGAATGAGATGATATCAAACAAAAATGAACTGGAAATCGTTATGATTTTCTTTATGAACCTTCGAAGTTAGTTTCTTTTTACAGATCAATGATTTTAATAACTGGTTCAGCTAGTGAAATTACAGCTATTAAAAAAGCATGAAATGAAAAAAACTGAAATCAGTTTATGAAATTTGGAATTGGTCATGGACAAACAAATTCAGCTTCACGTTTTGAGCTACCTGATCTTTTATTTAGAAAACATTTTGCTAAAAATTATCCCGGATTACAAAATGCAATTAATTCTGATCCCGATAAATTTGCCGTAGTTAGAGGAAGAGAGATAGGTATAAATAAAAACATCAAGATTGTTTTTGATGATGCTAATTCATTTTCTTGAACACAAAATATTAAAGGTTCAAAAAGACCTTTTTACACTCCAATTGATCCTAACGATAGATTAGAAATTCTCACTTATAGTGATCCGCTTTTGTATGACATTGGTATTGTTAGCAACAATTTATCAAGGATATATCAAAAAGCTATTGGTGAAATTTTTATTGAGTTAGCGCAATCAAATGAAGATCTATATGGGCCTTCAATTGGTTATAACGGCTATAAAATGATTAATGATTTTCAGAAAGAAGTTGTTGAAATAATTGAAAAAACCTATGGAAAATAAACCAATTCTTTCTTTTGAAAAAGTATCAATAATATACAAAAAAGCTCCACTTTTGCAAAACATTAGTTTTAAAGTAATGGCAAAGGAAAATGTTTGCTTATTAGGTAAGTCTGGAGTTGGAAAATCAAGCCTTTTAAACAGTGTTACTAATACGAAAATAGTTAAAAGTGGGTTAGTTTACTTTGATGGTGTTGCTTCAAACAAAAAGGAATACAAAAAACTGAAAAAACAGTGCAGTTATCTGGATCAAATACCAAATTTAATTGACACTGATTATGTATATGAAGCAATTTTAAGATCTGCTAAACAAAAATTAACTTGATTACAAAAATTAATTTGTTTTGAACCTAAATGAATTAAAGATAAGATCTTAGCAATACTAAAAGAAGTTAATCTTAATGATTATGTTAGTTACATTATTAAAGATCTTTCTGCTGGACAAAAACAAAGAGTTGAAATTGCTAAGCTTTTTTTTAAATCACCAAAGCTACTTTTAGTTGATGAACCAACCACAGGATTAGATCCTTTAACCGCTTCTAAAATAATGGATTTAATTACTGATTTTGTGAAAAGAGAAAAAATAACTTTGGTTTTTGTAACACATGATATAGATTTAGCACTGAAATATAGCACCAGAATTATAGCACTTAAAAATCATGCTTTAGTGTTAGATAAATTAACAGAAAAACTAACAAAAGAACAACTTTATAAAATTTATGATAACTAAGTTGTTTTTTCACCAAGTTGGCGATAATAAAAAACGCTTAATTTGGTATTGAAAACTATTAATGATAATTGCTGTTTTGGCTATTGTCATTTACAGTTGAATAGATAACTTTTCTAGCTTTAATCAGTTTGGTTTAAATGTTTTTATTAATAACATTACAAGCTTATTTACCCCCAATCTTAGCCACGAATATACACTAGTAAGATTCTTAGCACAAACTGCTTTTTTTGTTACGGGAGGTAGCTTTTTAGGATTTATTTTTGCGGTCCTATTTTCCTATTGAACTGCATTTAAAATTCAACCTTTTTACATTGCTCTACCGATCAGGTTAATAACTATAGTTTTAAGAGCATTTCCCGTACTTTTATTTGGTTTTTTATTTAGTAATTTATTTAATAAACAACTAGCAGCTACACTAACGATTAGCTGGTTTAGCTTTTTATGGAATACAAAATACATTACTACATTTTTTGAAAACAGCAATTTAAAGTATTTTTTTAACCAAAAAATTAGAGAAGGAAGTGGTTTTAAAGCTTTTTGAACCACTATTTTTCTTAGTGAAAATGAGCGACTATGGTTGTTTTTTCTTTATAGCTTAGAAGCAAATTTTCGTTGAACTACGCTTCTAAGTATATTTGGCATTGGTGGCATAGGACAACTAATTGTTAATCCTTTAAGTATAAGGGTGCAGTTTGATCTTGTATTAATTCCATTAGTTGTTTTAATAACCTTTCTAATTTTTATAGAAGTTGTTGTTTTTTTATTATCAAGTTTTATTTTTGAGAAAAATAGTGAAGATTTAAGACCAATTTTAAAAACAACAGTTATTGAAAAACGAAAGTGAAAAAGAATTATATTTATTTTGTTTATTGTAGTACTAATTAGTCTTAGTTTAGCCAATTTAGTGACTATTGATTACAGAATTAACGATGCTGAATTTTTACAAGATTTTTTTAACCACTTTTTTCAGTTAAAAAGTAATCTTTTTTCAAGTAATGATCCTAATATAAACCCAATTTTAATGTTAGTTAAACTTACAACTCAAGCTATTAGTTTAATTAGCTTGGTTGTAATCTTTTCTATTCTCTTTGGTTTTATATCATGTAATTTATTTAAAAAGAGATTTTCAATTAGTTTTAAGATCTTATTACTTTTTGTTAGAGTAGTTCCTAGTATTCTGTTGTTTAGGTTACTAGATCCTTTATTTCTAGAAGCAAAAACAACTATTATTTTAGTCTTATTAATTAATCATGGTTCAAGTTATGGCCAATTGATGGCAATTAACTTTAATAAGGCAAATCAAAATATCATCAATAACTATAAAAATCATGGTATGACAAAAGATTTTATTTTATGAAACTATTTGTTAGTTGAAAATAAACCTAATTTAATAAACATTACCAGTGATGCTTATGATAGTGGAATTAGGGATTTAATTTTGTTTGGTAGTTTTGGCGGTTCAATTATTGGTAGTAGAATTACTAATTTTTTTGAAAGAGCTCAATTTGATAATCTAGGTTCTGTTACAATCCCATTAATGGTTTATCTAATTGCAATAGAAATAATTTTTCTTTCAGTTAGATTAACTAGAATTTCAGTTTTTAAGAACTACCTTTACTAATGATAGTTTTGAAAGAAGCGCTCTAATATTAAAACTGCAGCTAATGTATCTTTAGCCTTTTTAAAATCCTTATGTTTTAAATCCATTGTTATAAGCTTATCTTTAACAGCACTTGTAGTATTACTTTCATCAACCAAAATTATTGGCAAATTAAAACGTTGTTCTAAAAGTTGTTTAAATGATTTAATTGCCTTTTGAATATCAGAATAATAGTGAAATTTGGGAAAACCTATCACTATTTTTTCAAGTTCATACCCATCGTTTTTAATTCGCAAAAATAAGTTGTTAACAGCAGTTTTGAAATTATTTTTTACTTCAAAAACATGAAATGCAGAAGGATATTTATCTAGTGTATTTGCAATTGCTGTACCTATTTTTTTCAAGCCAAAATCAATTGCTAATATATATTTCACTACTTATTTTTGAAACAATTCATTAAGCTTTTGAGGGGTAACATTATCCTGAAAAGAACCTCTAAATAACTTATCATTGCCTCCGCCCTTTAAATTAAAACTATTTCTTAATTTTTCAATAATGGTGGTAATTTTATTTCCTATAACAATAAATGAATTACTTCCATTGAATTGATTAATTATCAAGAAATTTTTAGTTTGATTTTGATTGAAAACATCATGTATTGTTTGTAGCAATAGTTTAGGTTCTACGTCATTAAAAGTAGCAATTACATAACTTTTATTTTTATCTACTAAGGATAATAATTGCTTTTTAATTGATAAGGCTAAAGCTTGCTGTGATACTTTATAGTTTTTTGTTTTTAACTGGTTAATATCATTTTTTAAAGCTAATAAAGTATCAGATGCATCTCTTAATTGGGTAATTTTTTCAGGTAAGATAAATTTATCTAGCCTTTGTTGCAATTCTTTTAACTCAACTTTAAAAATTGAACTATCAATCAAAGATAGAACTTTTTCAAGTTCTGATTTTAATTGGATTAATTTTTGATTTTCTGCTTTTAAATAGTTGTTAATAGTTTCATTACTGCTAATGATTTCAATCCTTCATCTTCCAGCTCCTAAAGAATAGAAATCAGTAATAAAACAATCTTCAATTGAAGCTGTGTTAGCTACATGAGTGCCACCACACAACTCAACACTATAATCACCAAAGCGGATCACTCTTAATATTTCATGTTGAGAATATTCCTCTTCAAAATAAGCAATTGCATTTAGTTTTTGACTCCCTTCAAAATCAGTAAAAATCTCTTCTGAGCTTATTTTTTGTTTAATCAAAGAGCGAATTTTATTTTCTACTTTCTCAAGTTCATTTCTTGTTAAATGACGATTCAAATTAAAGTCAATAGTTGCTTTTTGCGCAGATTTAAAAGCACCACTTTGTTTAATAAGTGGATCAATTTCTTTTTGTAAAGCTGCATGTAAAAGGTGTTCTAAACTATGGTTGTTAGCAGCAAGTTTTCTTCAAGTTTCATCATGTGATAAAGTTACTTGATCATTGAGTTTAAAACTACCTACTAAAAAGTAGTGGAAGTGTTGTTTATTAGGTCCTTTAAATACACCTTGAAAACTGATTTTTTGATCATTATTATTAGAATGATTAATGCAACTTCCTTCATCATATCTCTGTCCTCCAGAAGTAGCATATAAAACTGTTTGGTCAAAAACTACATAACCACTTTGATTATTAAGTTCTTTAACTGGTAAATAATTTTCATCAAAAAGACCAATTACCTTAGCATTAATTTTATTTTTGTGATAAAAAAAAGTACTTTTAGTTTTGAAATTAACTAAATTAATATTTTGTTTTTCAAAATTTATAGTTTGGTTATTTTGCTTAGAGATAGAACGATGTTTGGCCATTAACTGATCAAATACTGTTCAATCAATAGTTAAACCTTTTTGATTAACTAGTTCTCTTATTATTTCAACAGGAAAACCGTATGTTTCGTTCAATTGAAATGCTAATTGGGGAGTTAGAGTATTGTTTTTAACACTTTTTTCAAACAGCACTAAACCTAAATCAATCGTTTTATTAAATGCATTAATCTCTTTTAAAACTACCTGTTTAACAGTTTCATTTTTAGCTTTTAAATGTTGATAATAATTCTCATATGAAGCGATGATTTCATCTATTATCTTTTCAATAAATGCTAAGTTTAATTGCAATTTCTTACAAGCTATTAAAGCACGTCTTAAAAGTCTTCTTACTACATAATTTCTCTCATTAGGACCAGGTAAAACTCCTTCTGAAATGGTAAAAGTGATTGCTTTAAAGTGATCAGCAATAATCCGAAAATAACTCTGTTCTTTCACTTTTTGAGGATCGAATGTAAAGTAAGAGTTGGGATCATATTTAAATGGACAAAAAGCTTCAATTATTTTGATTAGCTTTAAAAAGATGTCAGTATCAAAATTGGTTGGACTATTTTGTAATACTGAAACAAGTCTTTCTATTCCAGCACCTGTATCAATATTTTTTTGAGCAAGTTCTGTATAGTTGCCATTACCATCATTATTAAATTGACTAAAAACAATGTTTCATATCTCAACATAACGATCATTTTCAATGTCCTCAAAGAAAAGTTTTTCACCAATTTTTTTAGGATCAAATTTCTCACCACGATCATAATAGATTTCAGTGCAAGGTCCACAAGGTCCTAAACCTAAGTCTCAAAAGTTACGACTTTTGTCACACTTAATAATGTGATTTTTATCAATTTTATGCTTAATTCATCTTTTATAAGTAGTTTCATCATCTTCATAAACAGTGATATAAAAACGCTTAGGATCTAGCTGATAATAATTAACTAAAAGATCAAAAGCAAAATCAATTGCTTCTGTTTTAAAATAATCACCGATTGAAAAATTTCCAAGCATCTCAAACAAAGTTTGATGTCTTGAAGTAAAACCCACATTTTCAATATCATTTACCCTTAAACATATCTGTGCATTAACAAGGCGTTTAGATGGTGGTGTTTTTCTAGCACTGAAATAATCTTTTAAAGTAGCAACTCCTGAATTGATTCATAATAATGATGGGTCGTTGATCGGAATTAGTGATTTTGAAGCTAAAACCAGATGGTCTTTCTTTGCAAAATAATCTAATCAGGTTTGCCTTACTTTATCAGTTGTTCAATTCATTGCTTAATTTTAAATGCAATTTACCAGTGATAAAAATAACTATAGTTCTTTTATTTGCTAATTTATTTTTATTTCAAACTTTTTGTTAGCAATTTGTGCATAAACATGATTATCAGCTAAGAACTGCTGAAACTTATTTTTGACTGTTTACTGTTCATAAATTTAAAGGTAAGTTAAGTTTTTTGATCATTTGGGGATACTTTTCATATATTTTGGTTCATGGGTGGAGGAATTGACAAATCTTTTGGATTCTAGCTGTACTAATTGTTTCAAACTGTTTTTTAGTCCAAAATAAAAAACCTTTTTTGTAACTATTATCTAAATCATAAACTTTTTGCAAAGATTCAAAGTTAAAGGATGAAAACAAGATCTTATCTATTGCTTTTTTACCATAACCTTTAACTAGGTCAACAAGCTTCTTTTCAATTCCTAAATATGGTTTTTGATCAGTTTTAATCTCAATATTGATTAACTTAAATTTATCTAAATAAAGATCTAAGAACTCTTTTAAAGTTAGGATCGATTGAAATTGAATTTTAAGGTGAAAAAAAGCACTATGATCATCTCTTTTTAAACTAACTAATGATTCAAACTCAACCTCCTTATTAACTAATGCGGTTCTCAATGTTGTCTCATCATGAATGATAACTAACTGTTCATCTTTAGTTAAATGAACATCAAGCTCTATTCCATCAAAACAATATTCAAAAGCTAAATCAAATGCTAGTTTGGTGTTTTCTGGAGCAATGAATGAATAACCCCTATGTGCTAAAAGCAATTGCTTGTTATGCATTATCAAGATCTCTTCAACGGTATGAAGCTTTATTTAATCGTTTAATTTCCATACTATTTGAGATAAAAACAATTGATCCACATATCAAACCAAACAAAGCAATGGATAAACAAATAGCAGCGATAATTTGATAACCAGTGTTACTAGTATTACTTTGTCCTGCAACTGTATATTTCTTACCAACTTCAGCAGTCACAGTGTAAAGTCATCCATCAGTAGAAAAACCAATAAAACTAAGTAAACCAACACTAGATGCATAGTTATTTTTACCAATATCAATTTCACCTATTTGGTTATATCTGACTGTAACCATTCCCCATGATAAGATACCAGTAAAGATATAGAGAATGGAAGAGAAAGTAATTAAAGTGATGTTAGCAGAATTTGTTTGTACAAAACCAAGTAGAATAAATGCTAGAACAAATACAATACCAAGAACAGTGCATATCATTAAAAACAAGATATAACTTTTACACTTATCTGCTAATCTACCAAGATAAACACTAACAGCACTTCTCAGTGCATAAGTTCTAATTCCCCCAATTACAGTTACTAATACTACAGGTGCTAAAAATGCATTTTGCAACATCTGTAAAAGGTAATAAGCAAAAGTGCTTTGAAACACATACATCCCCATTAAAAAGAAGGATAGTAACCATAACTTTCAGTTTTTTAAGGTCACTAAAATCTGGTTTAAATTGCGTTTAAAACTTACTAAAGTGGTTTGACTTTGTTTTTTAATTGGCTTTTCTTTAACAAAAAACAAAACTGTAAATCCAGTAATTACTAGCATTATGGCAATGATAAAAGCGTATGCAGCAAATGGTTTTGAATCAGCATTCTCACTACCTCCTGATGGATAGAAAATGCTGGTAATTATCAATGCAATTAGAAAGATAAATATTAAGCCTCATATTCCATTAGCAGCTCCCTGAATTCCAAAACCAAGTGCTTGATTTTCTTTTGTTGCTTGTTGACTAGCTAGTTTTCATAATGGTGTTCAAAAGATTAAAGTACTTGTTATCCCTCATAACCCTCATATTACACAATACTGAATAAATAAAGCATCATGGCTTTGTTGGTTTTTAGTTAAGATGTTGGCTGCTAATCAAGAAGTAATTGCTCCAGTAGTAATTGCTGATAGAAATAACAATTTTCTAGAACTGAAGCGATTGGCTAAAAACCCTCCTGGTAGTTGAGTGGCAAGCGTTACATAACCAATGATAGAAGTAACAGTAGCAACTTCATCCTCTGTAATTCCTAAGTACAAGTGGAGGTTAGGAACAACATTCTTAACATAATAGGGTGCAGCAATTACAAATACATCAATTGCTCCCAAAATAATTAAGGCAAGAATTTGTTGTTTTGAAAAATCCTTTAAGCGTTTTTTAAATCCTTTTTTTGTTGTCACTATCACTAATGATAAAGTGGGATAAATTAAATATACATCTAATAATAAAAGTGGATGGAAGTGAAACGTTCTTCACTTACTTTTAAAAAGAAAAATAATGTTTACTAGAAAAACTATTTTTTCTTAAATTAATCCTGCTCCTAAACAAATTTGATCAAGTGTATATAATACACCAAACTGTCCAGATGCAACACTTATAACAGGATTTTTAAATGTTATTTCCAGTTTATTATCTGATAATAATTTCAACTTTGCAATTTCTGGTTTTTGAGCATGTCTAATTCTTACCAGAACTTGACTAGGAAGTTGCTTTGGTGTATACAACCAGTTAAATTGATCCAATAAAATTGTTGTTTTCAATAATTCTTCTTTGTCACAAGAAACAAATAATTCATTAGTTTCAATATCCTTAGCAACAACAAAATGACGTTGTTTTAACCCCCCTAAATTTAATCCACTGCGTTGACCAATCGTATAAAACCAAACACCATCATGTTCACTAATAGTTTGTTTGGTTTTTCAATCCTTAATTAATCCTTTTTTTACAGGTAAATAGTTTTTTAAAAAATCACTGAAATGTCTTTCACCAATAAAACAAATTCCAGTTGAATCTTTTTTATCTGCAACTTCTCAATTATTTTCTCTAGCAATATTTCTCACCGTTATTTTTTTTAAATCTGCTAAAGGAAAAATAACATTCTGAAATTGTTCTTTTTTAACATTTGCTAAAAAATAAGTTTGATCTTTATTGGTATCTTTAGGAATAGAAAGCAAAGGCTGATTTTCTATCATGTTTATTTTGGCATAATGACCAGTTGCAAAAAGAGAATTAGGGTTAATTTGCTTACAAAAATCATGCAATAAACCAAACTTAATAAAACGATTACACCAGATGTCTGGATTTGGGGTTAACCCTTTTTTGAAACTTTGAATCATAGGTAAAAAAACTTTGTTTCAATAAGCTTCAATTAAGTTTTTTTTATTAACTTAATTCCTAAAGAATTAGCGATTTTTTTAGCCTGTTGGAAGTCTTGAAAAGATGAACAACCTGATTTGTTATTATTTATTTTCTTATGACCATAAAAATCATTATTAAGTGTCTCATCCCAACATTCCATAAAAACACCAATAACTTCTTGGTATTGCTTTTTTAAAAGTAAAGCACTAACAGCAGAATCAACACCACCACTTAAACCTATAAAAACTGTTTTTGCAATAATTGACATTTTTAATAAACAGCGTGAATAGCAAACTTGTTTACTAATCTTTGGTGAAATCTATTAGGATTAATGCCTTTTGTAATAGCAAGTGCAGAAATAAACACTATTAATGCTTCATAAAAATTTTGACAGCACTGCTGATCACTATCTTTAGGATTTTCTACAACTTTTGCTAGATAATCTAGCTGTTTAATTACTTCAGTTTGGATAAAAAAACCATCACTTAACATTGGTTTGAAAAAAAATGGTTGGTCATCATAATCATCATCTTCATAAATAACAATATTGTCTTGTTCTCGTTTTAAACACAAATTAAAGTTAATGCGAAAAGTCTCAAAATCAACCGCTTGAAATTCAGTTTGTAAAAATTTTTTAAAAGCTATGAGTTGTGGTTTCATTACACCAGTCTAAAAAGTTTTAACACTTCTTGTTCAAGATAGCAATCCTTAAACAGTTTATTTCAGTATCACCTAAATAAACTACTAACCAAAATACTGTAAAAGGTTTAATTTAAAAACTAATTTTCTTTATTATCTAAGTTTAAGTTATTAAGTAAACCTAAAACATATTTTTCCAGATCAAAGATAGCTAAATCACTAGTTTTTTCACCAAAACCTATCAGTTTAACAGGCAGGTTAAACATATCTTTAATAGCTAAAATAATTCCACCCTTAGCAGAACCATCCATTTTAGTTAAAACAATCCCTGTTAGTTTGGAAAATTCATTAAATACCTTTGCTTGTGATAAACCTGTTTGACCTACTGTACCATCTAAAACTAAAAGTGTTTCACTAGGTTCACTTCCACTTACCTTTTGAATAATTTGATAAATTTTTTGCAATTCATTCATTAAGTTAAGCTTGTTTTGCAATCTTCCTGATGTATCACATAAAACAAAGTCATATTTATCATCAATCCCTTTCTTTACGCCACGAAAGATTACAGCTGGAGTTTGTTCTTTAGGGTTTGGAAGTATAATGTCACAGTTTAACCGCTTTGCTCACTGATTAAGTTGTTCAATGGCTCCTGCTCTAAAAGTATCACCTGCAACAAGTAGAACACGTTTATTTTGTTTTATGAAAAAATCCGCTATCTTAGCTAAAGTAGTTGTTTTACCAACTCCATTAACACCAACAAAGAGATAAACATTTGTAAAGTTAGGTTTAACTATTAAATCAGTATCAAAGAGTTTATCTTGGATGTAATAAACAATGATTTGGTCAATAATTAGCTCTTTAATGAGTTGAAAATCTGTAATTCTGTTTAGCTTGATCTGTTCAATAATAGCATCACAAATTTTGTTTGCAGCATGATAACCAACATCAAGCAAAACTAACCCTTCAAATAGATTTTCTTTAAACTGTTCGTCAACATTAACATATCGTTTTGACAGTTCATTAATAGTTTTAGCGAAAGTTGTAGCAGATTTTTTCAAACCCTGATAGTAAGTTTTATTATTGGTTTGAAAAAGGCTTTGTTTTTCAACTTCTTCTTTAAGCTGTTTAGCAACTGATTTTTTTGGTTTTAGTTTTGCAATTAACTTGCTTAAAAAGCCCATTATTAGTTAATTAGAATCATTTTCAGATACATATTTTTCAGCATTTTCAAGTTCAACTGCAAAGGTTTTAGTTACACCTTTAGTTTGCATTGCAGCACCCAATAACATGTCACATTTCATCATTGTACCTTGACGATGGGTAATAATTAAAAATTGGGTGTTTTTGCTAGCAGTTTTAATGATGTTAGCAAAGCGCTCCACATTAGCAGGATCAAGTGCACTTTCCGCTTCATCTAAAATAACAAGTGGAAAAGCACTTACTTTCAAAATACTAAATAAAACAGAAAGTGCAACTAAAGTTTTTTCCCCGCCTGATAACAACATTAAGTTGGCAATATTTTTACCAGGAGGATTTGCAAAGACATCTATCCCTGAAACCAATACATTACTAGGATCAGTGTAACGAATTTGGCAAGAACCACCACCAAATAGATATTTGAAAGTTTTTGGCAACTCTTGATTTAATTTTTGGATCAGTTGATCAAACTCATTGCTAGCAATTTCATCAATTTCAGTAATAGCTTTTTGCAAATTTTCAACTGCTTGTTGAGCTGATTCATATTCAGCGTTAATATCATCAAAGCGCTTTTGCTTTTCACTTATCTCAGCAATTGATTCCATGTTAATTACCCCCATTTCATTCAATTGATTTTGTAGTTTAGCAATCTTGTTATGGGCTTGCATAGAAGAGAGTTTAACTGGTTTGTTGTGATTAGCAATAGCAAACTCCATTGTCATTTTGTAAACACTGTTAATCTTTTCAGTGATATTTTGGATTGTATTTTCAAACCTAATTTTTCCTTCCCGCGCTGAAACTAATTTAGCACGTTGTTCATCTAACAATGCCCTAAGATCAACTATCTTCTCTTCACTTTGTTTAATTGTTTTTGCTAGTTGTAACTTAAGTTCTTGATTAAGCTTTAACTTAGAGTTAATTTCATCACGTTTAGCTCAAGCACTATTTAATGAATGGATTAATTCAGCTTCACTAGCAACAGCTTTTTTACCATCAAAAGTTGTGTTAGTAAGCTGTTCATATTCCATTTTGAGCTCTAAAATAATTTTTTAATTTGAACAATACGCTCTATAAACTTGGCTTGTAACAACTCTTCATACTTGAGTTTTCTGTTCATTTCATCTAGCTTTACTTCTAATTCTGTAAGCTCTTTTTTTAACTCTCGTTCGTTGTTTTCTAGTTTGTTAATGTTCTGTTCATTATCAAGTGAAGCTGATGATAAATACCCATCACTAAGATTTGTTTTTTCAAATCCACCATTGATAATTCCCCCTGCATAAACTGTCTCACCATCTAAAGTAACGATCCTATATAACTTGTAAGTATAGTTAGAAAGGTTAATGGCACTATTTAAATCTTTAGCAATAATAACTTGTGCTAAAAGAGTATTTACAACTGGTTGAAACAATGGATCACACTTAACATGATCACTACAAACACCAAGAAAACCATCAAGCTGCTTTAAAATTTCCATGTGTTCGTTTGTGATTTTAGTATCACTAGCAACATCATCTAAAGGTAAAAAAGTTACTTTACCTATCTCATTTTTGACTAAAAAATCTATTGCTTGGATGGCAGCATTATTGTTGTTAACAACTAGGTAACCAATTGATTTTCCAAGTGCTTTTAAGATAGCTTTTTCATACTGCTTATCAAATTTAAGAAATGTTCCTAGTGTATTTAAAATTCCTGTTAAAGCATTAGCATTTTTAACTAAGATATTGGCATTGTTAGTTTTTTTAAGCTCATTAGTTTGGAGTTCAATAATTGTTTTTAAGGAACGTTGGTAAATTAAATCAGCAGTATTTTTTTCAATCTGTAACTTTATCTGATCAACAAGTGATTTTTGTTCGTTAATTAGTTTTTCAAGATCAGTGATGGTTGTTTTGGAATTTGATAGTTGGTTTTCAAAACCATCAAGCTGGGTTTGATCAACTAAAATTAGTTTTTTTAAAGCTGCAGCTTTTTGCTTTTCATCTTTTTGTGAAAAACCTTGTCTTAACTGCACATCAATAATGACTTTACGTTGTTCCAATTCATTGATCTTTTGGTAAATATCTTGTAACTCTTTTTGCAATTCATTAGATTGCATATCAGCACTATGAAAGCGACTATTAAAGATAACAATCTGTTCTTCTAACAACTCTAGTTGGGGTTCGTGTATTTTAAAGTCATGTTCACTACTGTTAATCTGAAAATTAAACTTATCAAGTTCAGTTTGTGCTTGGAGATATTCCCCAACCAAAACAGCAAGTTCCAACTCTTTCAATTCGTTTTTAACCCTAATGAATTGTTGTGCTTTTTCAGCTTGTAATGTAAGCTTTTTTAAATCTTTTTTCAGTTCATTTAAAACAACGCTAACTTGTTTTAAGTTAATTAAGGTTCGATTTAACTGGTTAACAACTTCTTCTTTACGCTTGGTATATCTACCAATTCCCGACGCATCTTCAAAGATCTTTCTGCGTTCTTCTGGCTTAGCTTCTACAAATCAAGAGACACTCCCTTGTGAAATAATTCCAAGTGAACCTTTTTCAAGACCAATATCAGCAAAAATACCGCTAATTTCTTTTAAAGTTGCTGGATTGGAGTTAATAAAATATTCACTCTGGCCACTCCCTCTATAAACCCTACGCATCACGCTTATCTCTTTTCTTGAATCATGTAATAAACGGTTGGAATTATCAAAGGTTAGCTCTATTTCAGCTAACTTACTAGCAGGTTTGTCTTTAGAGCCAAAAAAGATCATATCATCACCTGATTTACTACGGAGATGTTTCATACTTCTCTCCCCTAAAACCCACTTTAATGCATCAACAACATTGGATTTGCCCGATCCATTAGGACCAACAATACCAGTCATTGAATGGGTAAAATCAATTGTGATTTCATCAGCATATGATTTAAAACCATATGCGCGAAAACGTTTTAGAAAAACCATTTGTTCTAAAAAGCTTTAATTTGGATGTTGTTATTAGATTATACTAGTTGAAGTAAACTAAACTAGGCTAATTATTAGGTTACTAAAAAATAAGCTAATAACGCTTCATTTAAAAAAGCTTCTATGTTTTAATAGTAACTAGTTTATTTAATTTAAAAATTACATTAATTGCTTTTAGAAAAGCAATATCATTTAAAAGTTTTTATTTAATTGCTTGAGCGGCAGTGATAATTCCAACATTAAAAATGTCACTGACACTAGCTCCCCTTGAAAGATCATTCACTGGACTTGAAAGTCCAAGAACAATAGGACCAATTGCATCATACCCTCCAAGTCTTTGGGCGATTTTATAAGCAATGTTACCAGCATCTAAATTAGGAAAAACATAGATATTAGCACTATTTTTTCAAGTTAGTTGAGGTGCTTTTTGTAACCTAACCTTTTCAACAAAAGCAGCATCAAACTGGAGCTCACCACAAACACTTTGATGCAATTCAGGGTGTTTTTCTAAAAATAGTTTAGTTGCTAAAACAACTTTATCCACCATTTCACCCTTACCACTGCCAAGCGTTGAATAGCTTAAAAAAGCCATTTTTATCTCATCCTCATTTAAACTTTTGGCAAAATTGAAGGTGTTTTCAGCAATTGTTGCTAACTCTTGGGGGTTAGGATAAACAGCAAAAGCACAATCAGTGAAGTACAAACGTTCTTCACCTTTTTCCATGATGAAAACACTAGAAACAAAATTACCAGTTGCTAGTAACTGTAAAGCTGGTCTTAAAGTATCTTTTGTAGCATATTCTTTACCACAAACCTCACCATCAACAACCTTTAGAGCAACTAAGGTAGCAGCTAAAGAACTAGGATCACGTACAAACTTTTGTGCTTCTTTTAAATCCATCCCCTTATGCTTACGTTTTTCATAGACAAAGTTAGCATAGCTAGTTAAATCCATCTCATCAATCACATAATGAGTTATTTTTTTATCAAAATTTGCAGGGATTTCCTGACGATTATGAAAGATAACTGCAGGTTGGATCAGCTTAGATTCATTAAGCATTTCAACTGCTTTTAAAACACTTGCTGATCAACCTTCTGGAAAGATAATTACAGGTTTTTTACTAACAGCTTGTAATCGTTTTTTAAAAATATCAATAACACTCATTGTTTTATTAATTTGGTTTAAGAATTTTTTTGAATATCGCTAATACCTACTAACTCTTCCCCACCAATTAGTGCTAAAGAAGCACCACCACCAGTGGAGATAAAACTAAACTGATCAGATAGTTGCATTTGCTTAACTGCTGCAGTTGAATCCCCACCACCAATAACGCTAAAAGCAGTTTTATTTTTAGCAATAATCTCACCGATTTTTGAAGTTCCTTTAGCAAAGTTAGTAAATTCAAAAACTCCAAGGGGACCGTTTCAAAAGATAGTTTTGGCTGTTTTTAAATAACTTTCAAATAAAGCAATTGTTTTAGATCCAACATCTAGAGACATATAGGATTGATATTGTTCTTGAATTTTGTCACTAACATCTAAAGTAATGCCAGCTTGATCTTTAAATTCAGAACCCATTACCTGATCAATTGCCAGCACAATCTTATTATGAGTATCTTTATCCAAGATTTGCTTAGCAACATCAATTAACTCTTTTTCAACTAGGGAATTAGCAGTAGCTTTGCCTTTTGCTTTAAGAAAGGTATTTACCATCCCTCCGCCAATTAAGATATTATCAGCAAGTTTTAGTAAGTTTTCAACTACCTTTAGTTTATCTGATACTTTCGCACCACCCAAAACAACAACAAAGGGTTTTTGTGGGCTTTGAATTAGGTAAGAGAGGTTCTTTAGTTCTTTTTCCATTAAAAACCCAATACAGGATTTTGCAACATACTTTGCAATTCCTGCATTAGAAGCATGTTTTCTATGGGCAGTACCAAATGCATCATTAACAAAAATTTCCCCTAAACTAGCCCAGAATTTCGCTAGTTCAGGATCATTTTTACTTTCTAATTTAACAATTTCTCCTTTATCGTTTACATCACAATAGCGAGTGTTTTCAAGGAGAAGGATTTCACCGAATGCTAATGCTTGCACTTTTTGTTTAACTTCAGCACCAGTGTTTTTACAAGAAAATTGAACCTTTACAGTTGGTAAGAGTTGTTGGAGTAATTCAGCAACTGGCTTTAAAGATTTTTTGTTGTTTAGTTTATCTTCTAAACTCTTAATCCTTGAAAGGTGTGATAGTAGCACTATCTTGCAGTTCTTTTTAACTAAGAACTTAATAGTATCCAAACCAGCTAAAATTCTTTCACTATCACTAATAACCCCATTGATCATTGGGACATTAAAATCACTTCTTAAAACAACGGTTTTGTTTTGAAAATCAATTGCTTGGAGTGTTTTGAAATTAAGCATATTAGAGCTTAGCACAATAGCTAACTACTCTCACTAGTTGGTGTACATAGGAAGATTCATTATCATACCATGCATACACCTTATAAAGTTTCATGCCATCAACTTCAACAATATTGGTTAGTTTAGAATCGAAAATTGAACCATATTCAGAACTTACCACATCGCTAGATACAATAGGATCTTCACAATATTTAAAAGAAGCGGAAGCAAATCGCTTCATGGCTTGATTTACTTGTTCAACAGATGGACTTTTTTCAAGTACAACACTTAACTCTACAATAGAACCAGTTAACACTGGAACACGGAGTGACATCCCATTAAGTTTGCCATTTGCTTCTGGAACAACAAGCCCAATTGCTTTAGCTGCTCCTGTTGTTGTTGGCACAATGTTAACAGCTGCAGCACGAGCACGACGTAAGTCATTATGAGGAGCATCTTGTAAGCGTTGATCTGCAGTATATGCATGAACTGTTAGCATCGTTCCATAAACAATCCCAAAGTTCTTTTCAAGTACATGAACTAATGGTGCTAAACAGTTAGTAGTACAGCTAGCTGCTGAGATGATCTTATCATCGCTACTAATGGTTTTGTGATTAACATTGTAAACAACTGTCCTGATAGTTTTTTCTTTAGCGGGTGCGGAAATAATTACTCTTTTAGCACCTGCTTTTAAATGGAGAGAAGCACCCTCTTCACTTACAAACCTACCAGTTGATTCAATTACTACATCAATATCATGTTCATCCCAAGGTAAATTTTGGGGATCTTTTTCACTAAAAACATAAACCTTTTTTCTATCAATTTGCAAGATGTTTTGTTTAACAGTAATCTTTCTTTTCAATTCACCATGAGCTGAATCATATTTCAACAGGTGCGCTAAAACTTCAGGTTGGGTCAAATCATTAATTGCTACAACTTCAACATTTGCCTTACTGAGAAGAGAACGAAAAACAAGTCTTCCAATTCTTCCAAAACCATTGATTGCAACCTTAATGGTTCTATTCTTTGCTGCCATACTTTAATTTAATAATTAGATTTTAATACTTTAGCCTATTGCAATTAATTCTATCTGTCAAAAATACAGCAAATTATTGCTATTAATTGCTCTTAAAAAAATAAAGTTTTGCTTATCAAAATTGAAACTATAATTAGGATTTTCTCAGTAAACTAAATTCCAGTTATTAGCAATTAATAAAATACTAAATAAAAGTAAACCCACTAATAAAAATACCCCGTTTAATAAGTTGTATTTTAAAGGATAATGAGTTCTGTTAGTGTTATTTAAATCATAACCTTTAGCTTGTAGAGCAAACGCTGTTGTTTCTGTTTTTTTAACTGTTGAAAGTAATACGGGAATAAAAAGGGTTTTAATCTTGAAAGGATTTAAAAACGAACACTTGTTATAGATAAAACCTCTAGTAGCTTGAGCTTGTTTAATTCTTATAATTTCACTTTTAACAGTTGGGAGTAATTTAAATATAACTGCAAGCAAGATACTAATTGGTTGAACCTTAATATGAAATAACTTAAGAAACTTAAAAAACCTCTCAACTGCTCAAGCCAATTCATAGATAGAACTTGAAGTTGTCAGTAAAAAAGAAGCACTGAAAAGCATACTAATCCTTAATGCAATTACAAAGGATCGTAAAAATGAACGTAGATTAAAACTTCACCAGCTAACACCACCAAAATTATTACCACCTGAATAGATAAAACTTCCTAAAAAATTATGGTTCTGATCAACGCTTAAAGCAGTATTTGGTAAAAAGATAAAACCGTTAACAATTACATTAAACCACAAGAAAAATAACATCCAACTCAAAATAATTAATGCACTTTTAACCCTTTTCTCACTAATTACAACTAGGGTTAAAAAAACTAAGTTAATAATTACAAGACCATATAATCCTAGCGGTAGAAAAGCAACAACTAAACTAATTAGTCAAAACCACAATTTTAGAAGTGGGTCAATTTTGTTTAAAAAACTGGTTTTTTGCATGAAAATAAGTTTTTTAGTTCACTAATAAGATCATCAAGGTTTTTAATTTCTGCTTGGTTTTTAAAAGCTATTCCCTTTTCATTAAACATCTTAACTGCTTGGATAATTACTGGAGGGGTGATTCCATATTGCATCAACCAACTGGTGTTTGAAAAAAAATCATTAACAGTGGTTTGATGAATTATCTTTCCCTGGTGGAGATGGATGATTTTATCAGCAGTTTCAAAGATAAAATCAACATCATGGCTAACAATAACAATCCTTGTTGTTTGTTTCATTGCTACTAGCATTTTGCTTAAGTTGCTAATCGCCATTTGATCTAACCCAACAGTAGGTTCATCAAGGATTAAAAACTTAACTTGCATTGCTAACACACTAGCTAATGCCAAACGCTTCTTCTGTCCATCGCTTAATTCAATGGGATTTTTTATTAACGGGATGTTTTCCAATCCACATAGATCAAGGTATTTTTTGGCATAATTAACATCTTTTTTATGACAAAGTTTAAGGTTCACTGCTCCTGTTAAAACTTCTTCTAAAATAGAATCACAAAAAAACTGATCTTCTGCTTTTTGCAAAATATAACCAATTTTTTGCTTAAAATTTTTTGTTATCTTTTGTTGGTATTGGTTTGCAAAATAACAATAATTAGCACAATTTATATAGCCAAAACTAGGTTTTTCAAATAAACCTAACTGTTTTATTAGTGTAGTTTTACCACTACCACTTTCACCAAAAATAACTGTAATTTTATTAGGTTCTATTACCCCTGAACAACATTTAATGATCTGACCATCATTATGCTTATTGACAAAGACACTAATATTGTTAAAAAACAATGGATCTTGTTCAATGTTCAGTAATTTGCTATTAAAAAATGGAATGCTTTTTTTACTGTTTAAAAAGTAAAAAGGATTAATGATTTTATCAACCTTTTTATAAAAAGAAAACAGTTTTTTTGAATCTAATTTTGCTAGTTTAAGATTCGCTTTTTGAGCTTGAAGATGGTATTTTTGTTCAATTAAATCTAGTTTTATTTGAGGTGTTTGCATATCCAGTTAACTACATCATTCAATTCAAAACTTAAACTTGGTGTTTTTTTGTTTATTTGTTTAGCCACCTTATGGGCCAATAAAAGTGGAAAAGGGAGATTAAAGTGGTGGTTATGAAACAAATCAAGCTGCTCATATATCGCTTCAGGAGCAAACTTTTTAATTAGCGATCCTTTACTTAAAAAGATAATCTCATCTGCTAGAAACAGATCATTAAAATCATGGGTAATATTGATAACTATTTTGTTTTGTTTTTTGATGGTATCAAACACAAATTTTTTAAGTTTATTGCTGATCTTATTATCAAGCATACTAAATGCCTCATCAAGCAGATAAATTTGTGGTTTGACAGCCAATAAACAAGCAAAAACAGCACGTTGTTTTTCACCAAATGAAAGTTTTTTAAGTGGTGTAAATTGTTTATCTTTCAATTCAACAACAGTTAAAACTTCATTAATAATCTTATCCATTTCACTAGCTAAAACTCCATGGTTTTCCAAAGTAAAAATCAATTCCTGGTGGAGTGTATCAGCAAGTAATTGCACATCAGGGTCTTGTAATAAGATTCCAATCTTATTAAAACCAACAGAAGTCAACTCCTTATCATTGAAAAAGATAGTTCCTTTTTTAGCTTTTAGAAATCCGCCAAGGAGTTTAACTAGGGTTGATTTACCTGAACCGTTATGACCTATTACTGCTAAATGACACCCATCTTGAACATTAAAACTAATCTGATTTAACACATTGTTTTGGTGTTTTGGGTATTTAAAACTGAGGTTTTTAACTTGTAGCAAACTAAGCTTATTTATAAATTTTAAAAATAAGTCAGAGCTAAAAATCTGTTTTTTGGTAGATAAAAAACAAAAAAGCAGCTGTTGTGCTGCTCTTTTTTATTTGGAATATGTTCTATATGAACAGGTTATTTTGCTTGTGGACCTTGTTCACCAGTTGGGTTATATTTTTTTGCCATCTCTTCTCTTCACTTCTTAAAGTTTTCAATTTCCACTTTGAGTTTGGCATAGTCATTAGCTTTAATAGCAGCATCAATGTTACCTGTTAGCTTTTCTAACTTCTCTTTTTCTTCTTTAGGGAGATTCTTAGCATCAGGACTTGCTAATATCTCTTTGATGGTATTAACAATACCTTCCCCTTCATTACGTAATTCAATACGTTCACGGATGATGTTATCCCGTTCCTTGTTAGCTTCAGCATCACGGATCATCTTTTGGATCTCCTCCTCAGAAAGATTACCGTTGTCACTAATGGTAATACTGTTTTCCTTTTGCGTGGTTAAATCCTTAGCTTTAACATTTAAGATCCCATTGGCATCCAAACTAAAGGTAATCTCAATTTGGGGTTTACCTTTAGGTGCTGGTTGAATACCACCTAAGTTAAATCTTCCTAATGACTTATTATCTCTAGACATTGGTCTTTCCCCTTGACATACAACCACATCAACTGATTCTTGGTTATCTTGAGCAGTTGAAAAGATTTGACTTTTACTTACTGGGATAGTTGTATTTCTCTTAATTAAAGGAGTAGCCACACCACCTAAAGTTTCAATAGAAAGGGTTAATGGAGTTACATCTAAAAGTAAAACATCCTTAACATCACCACGTAAAACCCCACCTTGAATAGCAGCGCCAATAGCAACAACTTCATCAGGATTAATAGAACGGTTTGGTTTTTTACCTGGTACCATTGATTCAACTAGCTTTTGAACTGCAGGCATCCTTGTAGAACCACCAACTAAAAGAATTTCATTAATCTCTTCAGGTTTAATCTTAGCTTCCTTGATAACATCTGAAATAGGGTTTCTTGTTCTTTCAAGTAGTGGTTTTGTTAACTCCTCAAACTTAGCACGGGTTAGTTTTAACTCAACGTTAACAGGACCTTTTTGGGTAACAGTTAAAAATGGTAAAGAAATAATCGTTTCAAGTTGAGCGGAAAGTTCAATCTTAGCACGTTCAGCTGCTTCTTTAAGCCGTTGCATTGCCATCTTATCTTTTGATAAGTTTAAACCCTGGTGTTCTTTGGCAATGTAGGCTGAGATATATTCAATGATCTTGTTATCCCAATCATCACCTCCCAAACGGTTGTCCCCAGCAGTTGCAAGTACTTCAAAAGTACCTTCTGCAATGTCAAGTAAAGATACATCAAAAGTTCCACCACCCAAGTCATAAACCAAGACTTTCATCTCTCTTGATGCTTTATCAATCCCATAAGCTAAAGCAGCAGCAGTTGGTTCGTTAATGATCCTTTCAACATTTAAACCAGCAATCTTACCTGCGGTTTTAGTAGCGTTTCTTTCCGCATCATTAAAGTATGCAGGAACAGTAATAACTGCTCTTGAAATCTTTTTACCAATCTTTTTTTCAGCAAAGTCCTTAAGATAACTAAGGATTTGCGCTGAAACTTGTTCAGGACTTAATTCCTTAGTTGTACCATCAGCATTTTGGACTTTTACTTTATTTGAGGTACCCATCAACCTCTTGATGGAGACAATGGTATTTGGGTTTGTAACCATCTGTCTTTTAGCAGCATCACCTACTATAATTTCATTGTTTTTATAGGAAACAATGGAAGGTGTTGTTCTTTTACCTTCAGGATTTTCTAATACAACAGGTCTCCCACCTTCCATTACAGAAACACAAGAATTGGTAGTTCCAAGGTCAATGCCAATAATTAAACCATTGTCTGCACTCATACTTTTTTTTAGATCAATAACAATTACTCAGTAATTATAAACAAAAATTAGCACTCAAACACTAAAAGTGCTAATTAAAGTTTGTTTTATTTATCTTTTTTAGTTTCTAAGCTAACAGTGTTAACTGTTTTCAACGGTACATTAACGGTCTTTTTAAATTCCCATATAACTTGAACAATTCACACAACAAAACAAATCCCAGTGAAAATAGCAGGTAGATAAACAGATAAAAGTCTTCATAAAAAAACACCATCTTTAATCTGATCATGGAGGAATTGATCAGTTGGTTTGAATGCATTGAGAAAACTAGTCATCACAAATTGTGTTGCACCTTCCCCACTAGCAACTGGAATAAAGTTAGAAGCGGTAACAGCAATATTAGTAATGTTAAAAAGATCAATTAAGCTGTATTGATCAATAACGTTATTAGTTGTGTTGACAGTTTTTGTGATCATAAAAACACCAAACAAACTAAAGTAGGAAACAATTGCTACAACCATGTTAGCTAGTAATTTAAAGATTGTTAACCCTCACCTTCGCATCTCAATTCCATACAACTTATTAAATTCAGCTTTTTCTATAAAACGTTGGTAGATCTGCTCTTTAGTTAAATAAGGACGTTTAAGTCACTTTCGAAACTGGTTTACAAGTGAATAGATCAAAACATGCATCTTTTTATTGAAAGCAATAACAATAAATAAGATAGCAACAACAACATCAAAGATCATCCCAGTAAGACTTAGTCAGTAAGAGACAAAGCTATTATGGTTATTTGCTAACAATTGATAGTTTTTAGAAAGAACAAAAAAAGAAGGTCAAGTTATTAACGCTTGCGATAGATTTCAAAATGCTCCTGTAGAAGTGACAATTAAAACTGCAGTTTGTTTTTTCATCCCCTTTTTAATGAATCAATACAAGCGAAATGGATCTTGTCCTAGTGATAAGGGGGTTACAATTTGAAAAAACTGTACTACAAAACCAAATAATAACCACTCTCACCAACTAGCATAAAAACAAAATCTCCTTGATACCCACCAATTAATGATTACATTCCACAAAACTGAAACTAAAAACCCTAAGATAACAACAAATATCCAACCCCAATTCTGATAGTTAATTGCAGTGATAATAGTTTTCACATCATCAACACTAACACCTAAAAAAAAGATAGTAACAATAACACTAATAACGATTAGAAAAACCAAAAAAAAGCTAAAAGCAACGATGTTTTTAGTGTTAAAAAAGGTATTGGTGGTTAACTTTGCCATTACTTAAGCAAGTTTAAGCCTGATTGAATTCTTTTATCACCAATCCTAAATTGGGGTTTTTCAATTGCACTTAAAGCAAGTTGTTGGGTGCCTGTATCACTAGCTTGAAATAAAACTAAAGCTGCTACTATATCACTTTCAATATTGGTAGAACTTGTTAAACTAAGTGAATCAATGTCACTTTTAGTGAACTGAAGATAGTTGTAACTATCACCACCAAAGTTAAATTCACTAAATTTTTGGTTGGTTTGGGTACTTCTTTTTGAAATACTTGGACTATTTGATGAATTAAAATAACTGGAATATTGGTCTAAAACAGCTAATAACAACGCTTGTTTAGCTTTGAAATCTAAAGCAGAATTGTTAATTGCATCGACCTGAAAATAAGCTGAAAGTGTTTTGCCATTACTACTACTACTACTTGGAGTTAGTCTACTTAGCTGTCGGGCAATGTTATCTAAAACTAATTGGTCTTTTGTTTCACTAATAAATTTTTTAAGATCATCCTTTGAACCATATTGTTTTAAGGAATTGGCAGCGATATGGTTTAAAAGTGCCTGGTTAGTATTGTTTTCTAACATGGAACTGTTAGTTGTATTCAAACCAGTAAAACCATAAGTACCATTATTACTTGTTGATACTGCTTGTCTGGTGCTTAACATCGTAGTAGCAGGTGTAGTGTTAGGACTTGTTTTGGTTTTAAAAAAGAAATTATTAGGATCAATAGTTTGGTTGGCATTAGCTGTTGTAGCATGTTCATCATTTTCATAGTTAAAAACAGAGCCAAATATTCCCTGAGGATTATTGGTGTTGTTACTGATAATATTCTTATCATAAGCAATATAGGCAATTTCACCATACTTTAAGTTAGCTTGTAAAATATCTTTAAAGTTTTTTAGGTTGTTTTCAATTAACCATTGCAAAGTAAAAATGAAGCGATAATGGTCAATAACTGCTTTAAAGTTAACATTAAAACCTGATTCATCAACACTCTCTAAAAAAGCTTTTTTAAACAATTCATCAATATTGCCATTATTAGGATTACCATACATGGCTTTATTGGTTAATTTGCTTCATGTCATGTAATAGTAATTGCTAATTACTTTAGCTAAACTATCTGCATCACTACTTGATGAAGTTGATGAAGATCCATTTTTAGATTTAATCTCTTCAAATAGCTTTTTAGCTTTTTCAAACTGTTGATTGCTAGTAAAAGCATTTTTTAACAATGCAAAGGAAAGAGGGTTTACAGTTGTTACATCTAACAACGCATTTAAACTCCAATTGGCACTGTTAGTAAGTGATTGATCATTGTTAATATCAACTAGAATAATGTTGCTAAAGTTAGGTTTTGATTTTTCTGTAACTTTCAATTGGTTAACAAATTCTGTTACTTTGTTATTTATGTCGCTCAGAATTTCATCTCTTATCTTATCAACATCAGCTTTTGTAGTTTTTAACTTATTGTTGTTTTCACTAATATTTATTTTTTCACTACCTACTTTTTTCCAAAGTGTTTTAGTAATTAAAAACAAGAAATAGTTATTGAGTTTTTCATAATGACCAGACTTGCGAACATATGGCAAATGGGAAGATAAGCCATTTAACCAAGGGGTTGGATTAATTTGGTTAACATTGGTATTAAGTTCAGTTGTTCTACTTGTTAGTTTGTTATCTAATGCATCCATTTGTAAAAACAAATCTCTGATTACATTTGTTGCTAATGCAACTGGTGCAAATTTTTGCATTAGTTCTTTGTAATCATTGAATGCTTTTTGCATCGCATCACTACCTTTATCTTTTTTTTGAAAATCAGTATTTATTAGTGCGTTTACTAATATTAATTCCTGATTTTTTTCAAACCAAGTTTTCAGTGTTTCATTTAATGGAAAACTAAAGGAATATAAACTGTTTTTAAAGGTGTTTGTTTTGGCTTGAAAAGCTCTTCACATTAAAAACTGCTTTTGCTTTTCAATGTCTCTTTCAGTTTTGCTACTACTATTTTTTGTCAGATAATAACTACCTCCATCAATTCCAATTACAGCAACTCCCTCTCCGTTTCTAACTAGTCTTAGCTTCAATTGGTTATTAGAGTTATGGCTTGTATTTTGAACAGTAGCATTTGCACAGTTTGCACTAACAGTGCTAGCACCATTATTTGTTTTGTTATTAGGAATAGCTGCATCCCAAACAAGATAACTGCTTGTTGTTGATGAACTAGCTTTAGTTGAAACCATCATATCACTTGTAGTTGTTTGGTGGATTTGCTTTGCTAGTTTTCCTAAATTGGAACTATCATTAGAACTTATAAAAGCATCTTTAATAAGACTCTTTAAATTGTTTTGTGAACTGTCTGTTGTATTGTGTTCACAGAACAATGTATTACTCTTAGTATGGTTTTCAATTTTAGTTGATTCAACAACAATGGTATTGTTAAGTTCTTTATTAAGCTCTTTAATTGTGGGAAGTTGATCTTTATCTTGATTTAAAAGATCTAGTTTTAAATTTAAACTAGCACCTAAATTAGCTGCTTCTAGTTGATCAACAATGTTTAGACTATCTACTATTTGCTTGGTTTGAGTGTTATCAGAAAGAGTTGTTGGAAAATCAATCAGGTTGCCTTGATTTTGATCTAAGATGTATTTTTCTAAACCTGTTTTTGCTGTATTTAATCCGTCTTTTCCCTCAATTAACTGCTCTCAGCGTTTGTTGCCAACATTCTGGTTCTGAGGAATTTCCCCATCTTTAGGAAAGAAAGGAAACGCATAAGAAGGGGTTAATTTATCTTTTAGTTTTTCAGTGTTGTAAACTTGACCCAAACCTTGTTGTGGTGCTGCATATTTAAAAGTAGCTTGACTGATTAAAGTAGGATCGGTATATTCAACCCAGAGATTAAAGATTTGTGCTTGTAACTTTGCAAACAATGCATCATTAGTTTCACTAAAACGTGGTTCTTGAAAACCAAAGTCCTTTCATTTTTCAGGTTGGGATAGTTCATCATAGATATAAGGACCAGTTGAGAGTTGTCCATTAGGTTTATAAACTAAGAAATCCTTAGCGAATAATTTAGCGGTAAAATCACTAATAAGTTGATTGAAAAGTTGTTGGGATTTTCAACTAGCCTCACTGCCACCACTTTTATCTAATAGATCTTGTTGAATAAATACTGCTCTGTTTCTGGGTGGTGATATTAACCCATTAACAGTATTTTTATATTGATCATCAACATTCTTTTTAGTGTCTTTTAAAAACTTGTTTATGGCTTTGTCTTCATTGTTTTCATACCAAGCCTCAAGTGCTTTTAAAAGTGGTCCTATAACAAAATGTTTGTTTGCTGAAGGAGTTTTTAAAGCAGTAATAAGTCCTTCTTTTAAACTACCCTTTTGACCATGAAAAAAATTAGTTGAGGTTGGTGAAAAAACTGTTTGCAAAGCACTGTTTGTTGGAGTTGAACAAGCTACAAGTATTGTGCTTACTGACAAACCACAAAGTAAAAACCACCAGAACTTTGATTTTTTTAAAAGTTTTTTCATGATCTTGGTTTTTTCTTATTAATTAAAAGGTTTTGAATCTGTTGTTTTTGGTATTTTTTGTAGTTAGGTTTAACCTTTTTATATTTAGCTTTGATAGCTTTAATTTTCTTAATTTGTAAAGTTGAAAGCGGTTTTTTGGTAGTTTTTTGGTTATTTTCAGTCAAAAACTTCAAATCACCATTACTAGTTAAACTAACGTTTTGAAATTTAATTCCTTTTGCTATTAGTTTATTTAATTTACTTTGATTTTGGTCATTTCAAAAAAGATATGATCTGCCCCAACTGTTAAGTCGTGCTACACGACCAGAACGGTGGATGTAAAAACTATCAATTTTAGGTAGATCTCAAGAAATCACCACACTGAAATAATTTAAATCTATCCCACGTGAAAATAGATCAGAAACAACTAGTAATTTCAATTTATTATTAGTTGCTTTTGTGAAATTATTTTTACGTTCTTGATAGGTTAAACTACCATAAATTGAACCAAAACTAATATTGTTATTTGAAAGCAATTGTGTTAATTGTTTTAAAGATTTTTGATTAGAACAGAAAACAATAATTTGCTGATTTTGGTGGTGTTTTAATAAGGCTAATAAACCAGAAAAACGGTTTTCAGTATTGAGATGAACAACAAAATGCTTAACTAAGGGGTGAATCCATTGCTTTGGATGGAGATTAATAACTTTAGTATTAATTACTTGTTTTTTAATTATTTGGAGTTGTTGATTGAATAAAGTAGCACTAAAAAAGGCAAACAAGGGCTTTGCTTTTTGAAAAAGATGAACACATTCAAGTCACTGCTGGATTGAACTTTGCTCTAAAAACATGTCAATTTCATCAAAAACAACGTATCTAACTTTGCTAGTAAAAAGGTATTTTTCGTTTAGTAAACTAACAATTAGTTGTGCTTTTGAACTAAATGAAGTGGCAAAAGTAAAGGTTTTAAAGTACTTTTTGATTTCAGTTAAGATGTTGATAATTTGTCATTGTAACTCTTTGGTTGGTACAAAAATAACTGCTTGAGGTTGATCTAAACTGGTGTTAATCTTATCTAATAGAGGTAAAAGATAAGCAAAGGTTTTGCCACTACCAGTTTCAGCAATACCAATGATATTTTGGAATGGTCATAATTTAAAAACTGCTTGCTGAATTTTGGTAAATTCAACAATGCGTTTCTTATCTAAAAACTGCCTAATAGAAGAGGAAAATTGCATCTTAAACTAGAATTTACGCACTCACTGTGAACTGATTGCTGAAAACAATCTGTTATCACCAACTCTAACTAAGCCATTTTTGGCATTTGCTATCAATGCATTAATAGCTTGGGTTTGATTATTAGGATTAAGCGCTTCAAGCGCAACTGTACTTAAAAATTCTTCAAGTGAAAGATCAAGTCTTTTTGAATTATTTGAATCTGTACTATTCAAACTATCACTACCAAGCATACTTACATCTTCAAGGTTAATCTGTTTTGCATAAACTGCATGATAGATCTGGTTATCTTGTAAAAAGTTATAACTGCTATAATTACTGGTTTTATTTGTACCTATATACCCTTCAAACCTACTAAACACATCATTAAAGTTACTATTATTAGTTTTACTTTGAGCTTGTTTTAATTGAACAGTTTGAGTATTGTTAAAGTTTGAATTAGCAAAATTCTTCAAATTAGTTTTTTGTTCTTGTAAGGATTTTGATCTATCAACACCAGTAACGTTAATATTAAGGTTACTTGTTAAATGGTTATAAAGTGCATCAAACTCAGCTTGGGTTGGGATGTTATCAATTGTTTCTATAAGTTTTGTTAGAGAACCAAAACTAAATAAAGCACCTTTGTAAATTGTTTCATTACTACCACTTGATTGAGTAGATTGTAATTGTGCTTGACCACCATTAGAACTTGATTTTTCAGATTGGGAAACAAAACTTGTAAAGAGGCGATTTCTAACTGCATCTGGTAAGTTGTTGGATGAAGTGTTGGTAACAATACCATGGAAACCAAACCTTCTTTGCTGGTTATTGGTTTGCTGTGCACTTTTTTGCATTAAAGTTAATGATGTACTGCTACTACCATTGTTCTTTGTATAGTAAAGACTTGAAATATCATTTGCAGCACTGTTAACTGTTGGGGTTTTGTTGTTGGTTCAATTATAAGCACTCGAACCAAATACATAGTTAGGATTTTGTTGTTCTTGCTTGGTAAAGTCTTGATTAGTTTGAGAAGCAACGTTGTTAGCCATTTTTACTAATACAAATTTAGAAGAACTTACACTCATTGCTTGACTAGCAGTATTATTTCTGTCTTTATCACTAGCTAAACTTCAAACCAAGAAACTATTTGTTGTTCTTGATAGTTTAGCTTGCAAGTTCTGTTTTAGGTTTTTAAGGTTATCTTTTAATAGCCATTGAACTGTATAAAGATAAGAATAGTAATTGATTAAGTCATTGCTATCCAAGCGTTCTTCTAAGTTTAAGCTGTTGTATAAACTATCTAAAACCCCATTAATTTTGGGTTTATTATCAGTTGAACTTTCATTAACACCATTACCAAAAATAGAAGGATTAGCTTGTTTTTGAAAAAGATCAAATAGGTAGGTATATTTAAAGATATTTCAGTTTTCATTATTGAGACTGTTTCAGTTTTTAAAAGTTAATTTATCTAAATCAATCGCTTGTTTAAACTGATCATCATTGACAAAATAATCTCGTCTAATGGTATTTTGTAACTCATCACTTGATAAAACAGCATTTAAAGCTAAATTAAGTGCTAGAGAAGTGTTTCTAGGATCAGATGAAAGTTTGGTATCAACAAGGATAATTTGTGAATATTGCGGTGAGGGAATTACCGAAACTTTTAGTTCATCAACATGCTTTTTAACTGCTGCTTCAACTTTCTTTTTATTCTCTTTTAACTTGTCAATTATCTTTTTTGAAAAAGGATCACTACTACTACTACTATTTACTATTACTATTACTATTACTTGTATTTGAACTAACTTTATCAATGATGTCTTTATAGTAAATACCAAGTTCATTGTAGTGTCCTGTTTTACTATCTTGTTCATAAGGTAAAACAGCAGCTAAACCGTTCATCCATATTTGTTTATTTCGCTCATTATCAATGAAAGGTTGGTTACGTTCTGCTAGTTTTAAAGCAACTTTGTTTTCTGCAGTAGCAACTTCATTAAAAACAATTTGTTGATATTGACTTTCAACTAAATCCTTCAACTCGTTTTTAACAGTGATAATGCTATCACTAAACTTTTTAAACTGAGGGAAACTTAAAAAGTTACTTTCTTTGCTGTCTACTAATTTAAAAAGGGCATTAAATAAAAATAAAACTCTGTTCTTATCAAAGTGTTTTTTCACTTCATCAAACAACTTAAAGTCATAAGTAGTGTTTGTATCAATTAAGCCATATTTGGTTTGTAGTGCACGGAATAATAAGAATTGTTTTTGTTTATTTACATCACGTCCTGATTCAGAGAGATAATAACCACCACCATCAACACCCATTAGATGGATACCATCTTTACCACGTGCTAAGATTAAATCAGGTTGACTATTGTTATCTTTTTTAAGTTCTTTTAGATCAACGATTGCAGAATTATTACTACTATCACTGTTACTTGTACTAGTAGCATTACTGAACTTTTTTTGATATTCACCTTTAAAAACATCTGTTGATTTAGTAGTTTCATTTTCTTTAAAGATCTTAGCTAGATCTGTTTTATGAACAGTAGTTGTTTGTGAACTACTACTTGCACTTTTCATTGCAGCCTTAGCTGAATTATTTTCAACAAAGAAATTTTCAATGATTGATTTATCTTTTTCTAATTTAGTGTATTCAGTTTGTTCTGCACCTTTGGATTTTTTTTGTAAAGCTAAATAGCCCTGGATAAAAGCTGCTGAAAAGCTAGGATCAAAGGTATCAAACATATCACTAGCTTTTAACAACAATTTACCACCACTATCACTAGAAAACTTATTAGGGATGTCAATGGTTTTATTGTTAGTATTTACATAGCTTTGTAAATTACTAGCTAGGATATGAAAACCATTTGCTCCTTTTATGGAGTTATCTTGTTGGTTATATTTGTTAAATACTTGAAATTGATAGGAAGGGGTTGGAGATTTAATTAGATCAGGATTAAAATAGTTATCAAACCCATCATTAGGAGTTTCATTAGTAAATACTACTCTTGAAACTAAGTTAGGGTTTTCATTAGTAACGAACTGATCTAATAATTTATCTTGAATGGCAGCATAAACTGCATCATTATTGATCCTAAGTCTTTTATTTTTATCAACAAACTTAGCTTGTATTTTGATATTATTCCAATTACTTTGGTTTTCAATTAAACCTTTTAATGGAGTTGATAATACGCCAACACTTTTATCAACATATTCAACAAAGTTCTTTGTAAAAAGCTTATTGATAAAATCATCAACAATTTTGTTGTTAACATCACGGAGCTTTCAGTTGGCTTGATTTCCACCAGTGTTATCTAAAATATCAGTTTGTAACCTAACTAAATAATCACCCCGATACTGATTTCTTAAGTTTTGTTCTTGATTAACAAAACTATTGTCTGTATCAGTATTAAAAGTTCTTAAATTTCTCTTAATGTCATCATCAACATTTTCTTCATAAAAATTGCGCAATACTGGCGCTAGGCGCATCGTTAAATATTGCGTTAGTCCTTCTCTATTTTCAAGTGCTTTATAAAGTGTGGCATTAATACTACCATCATTTTTATCAGTAAAAGCACTACTGGGTCTAAAGAGATTTTCAATGGTTCTGGAGTTTGGTTGTGCACAGGCAACAGCAACAATTCCCAAACTTACAGCTATGCTTGAAAGCAGCAATGGTCATTTTACTCGGTGTAAAAAACGTTTCATTGCACAGTCAAAAATTTCAATAATTTTAAAATTTATATTTTTATTATTACCACATGCGACTTGAAATAGAAAACGGGCTTGAATTTGTCAATGATCCTGTGGTAAATGAACTTGGCAAGATCTGTTTTTTTCATCCTTTTACAGGTAATTTAACAAACAAACTTAGTTTCAGAAGTCATTTCAATAGATACAGTTTTTATGCCATTAACTACCCAGGGCATGGTAATAGTGTTATTAACAATCCAAAGCAACTTGAATTTAGTTACTGATTGGAAATTACAAAACAGTTTTTTGATAAACATAACTTAAAGGATGTAATCTTATTTGGTCATTCTATTGGCGGTGGTTTAGCAGTTGCTTTAACTAACTATTTAAGCAGTGATCAATATAAAGCAGTCTTGTTAGAAGCACCATTAAATCCTGCTATTGTTGAAACTCCTTTAAATATTGTTCAAAATCTCATTCCTGATCCTGATAGTGATTTTGCTGTTATTCAAAAGTGTCTTGTTTACAATATTGAGAAAAAACTTGGGGCAAACTTTAAAGAATATTGTGAAAGAGAAAAGCAAAAATCAATTCACCAAAACCAACGCCTTAAAGTGATGTTAGAACCTTCTACACTCAAACAAAACATAGTTTTAATCAATGCAGCTTTTTTAAAGTTAAATTGTCCTGCTTTGTGAATCCATGGTAAGCAGGATGGAATTATTAAGTACCTTCCATCCAAGGCTTATTATGAATCATTAAACAATAAGCAAATTCAATTTAAAGCCATAGAAGCAGCGGCTCATACCCCTTACTTTGAACAACCACAAAAGTTTTTAAGTTTAGTTAATGATTTCTTTCAATTAATAAGTTAAAGCAGTTTATTTAACTAAACGCTTGTATCACTCAACAACGTAAGATTCATTTATTCCAGCAGGTAGTTCGCTACGCTCTGGGAATCTTATATAAGTACCTTCAAATGCCTTTTTGTTGGTTTCCACAAATGATGAGATAACACTGTTTTCAATAAAATTTTTAACTAATGGGGATTTAGTTATTCTTGCTTTTAAACGGACTTTATCCCCGGGATTAATGATGATTGAAGGGGTGTCAACAGTTTGATCATTTAAAATCACATGACCGTGGTTTACCATTTGTCTTGCACTCTTTCTTGTTGGTGCAAAACCCATTCTGTAAACTATGTTATCCAAACGTGATTCTAAAACTCTAAATAAATTAACTGTTAAGTTACCTTTTTGTTTTAAAACAAAGCGAAATAACCTACGAAATTGTTTATCAGTAATACCATACATGTATTGCATCCTTTGTTTTTCTTGGAGTTGTTGGGCATAACCTGATAAAGTTGAGGAACGAAACCTATTTCCATGTTGACCGGGAATAGATTTACGTTTTTTTCCTTTGGAAAACTCTTTGTTGTTTTCAAGTAAAGAAAAACCTAAACGTCTTGATCGTTTGAAAATACTACCAGTATATTTCATGTGTTTTCGATTTTAAATTATCTTAATAATCAAGGCTAAAATCACCACTGCTTTCAAATAAGTCTTCATCTAATAATGAACTGAAATTGGAAGTACGATCAGGAACTAGTGTTTCTATATCCAAATCATTAACACTAAAAGCACTCATTGAATCCATTACTTTGGCATCAATGTCAACAAAATCATTGTTAAAACCTTCAAATTCAAAATCGATGTTACGATCCATGTTTTCATAGATAAAATCATCTAACTGTTCATTGGAGATGTTATCAAAAGTGTTGGAAAAATCTATTAAATCATCCATTAACTGATTAGGTTCTACTAACCCATTATCTGTTACACTATCAAAATTGCTTAAAAGTAAAGGATCTTTTTCTTTCTTTGCAAAGCGGAGCGTATTATCAAGCTTATTAATGTTGTTCATAATAGTTTCACAACAAGAGTTATAAACAGTTTTAAGTTGTTTTAAACTACCTTGAATGCTTTCTATCTTCTTTTCATTTTCAGTGATAGCACGTTGAACATGACGCATAATTTCTCCAGATTTTTGCAGTTGTTCATAACTATTACTTGGTTTGTAAGCAATAGCAAAATCACTAGCTAATGATCTGATCATTTCTTCTTTTTGAAGTGTTGTTAGATTCTTAGCGTTATTAAATTGAACGTTAAGGTTCTCATTTTGATCAATTAAACGGGAAAGTTCACTTTGTAATTCCTTTTTAATTATTAAGTAACGTTCACTAATTTGGTTCTTATAGTTGGTAACAAAATCATTGAGATGGATAAATGATTGTTTAACAGACATCTGATAATCATTCATTTGAAAGATTGCATCAAAGTTAATTGTAGGGTTTAATTCACCACGTTTCAATTCACTAATAATCTTGTAAAGATCTGATTTTTTATTGAAATTATCAAGTTCACTTTTATCTTCAACCGATTGCTTATTTTGAGTAATTGAGTGTTCTTCAACAGGTTCTTTTGCTTTGACAAAAACTTCAATTTTAGGTTGTTGGTCTTCGCTATTAACAGTATTAATAGTTAGTTGATCATCTTTTTTATCGAGTCTTACTGATTCACTAGTAACAATTGATTGATTATTTAAGCTATTAATTTGTTGCAATTGGACAGATAATGTTTTTGCAAGTGTTTTGGAATCTTCAGTTATGGAATTAATGGTAATTTGTTCTTGCTGACTTTCTTTATTATCTAAAGCTAAAGTTTCAACTGGTTTATATTCAATACGGTTGGATACAAAACTAATAGGTAAAGTTGTAGGGATTTGGGGTGAACTTTTTTTGGTAGTGATAGTTATCTTATTATCAGACTGAATATCAAATTTCTGTAAATTATTAGTGTAGTTACTAGTTTCAACTACATCTTGTTTATTGAAACTACTTTCTAAAACTGGCTCCTTAGTTATTTCAGATTTGAATTGATCGTTAAATTCGCTATTTACTTGATCAAACTGCTCAACTGTTGGTTGAGTTTCAAATGAAGAAGAAAAAGTTGTTTGGTCTTGTTGATCTGTATCAATTGGTAATTGGTAACTATCAACTACTTGGGCTTGTTTACTTTCAAAATCAGTAGTTACTTTTTTTTCAGCAGTTGGTTGTATTTCCTCATTATTATCAAGATTAGCTTGAACTTGATCAAATCCAACTTGATAATTATTTTGTTCTTCAGCAACTGGTTTGAGCTCTTCATGGTGATCAAAGTTGTACTGAACATGATCATCTAAAGAAGGTTGGTCAAGGGTTGGTTGAGGTAAATCACTGTCAAAGCTGTAGCTATCAGTTGTTGATTCAGGTTGTTTTGCAAAATGATCATCTGAAGAAGGTTGGTCAACAACTGATTCAGGTTGTTTTACTTCTGGTGGTAGGTCGTTATCAAAACCATAGTTTTCATCAGTTGGTTGTTTTGCAAAATAATCATCTGAAGAAGGCTGGTCAAGAGTTGGTTTTAGCTCTTCATTTTGATAAATGTCGTATTGAACTTGATCAACTGGTTCAGATTGACTTTCAAAGTGTGCATCAGGTGCAGTTTCACTATTAAACTTAACAGGTTCAGACTCTAACGAATTATTGCTGTTTGATTGAGATAAATCACTCCCAAATTTATAGTCATGAATTGGTTCAGATTTACTTTCATAATGCACATCATCAGATGCAGTTTCACTATTACTATTAAGTTGAATGAATTCAGAATTTAATGAATCATTGTTAGTTGCATCTTTGTTGTTTTCAGAGTAAAGATTAGATTCAGAATTTAAATCACTAGTTTCAACTACATCTTGTTTATTGAAACTACTTTCTAAAACTGGCTCCTTAGTTATTTCAGGTTTGAATTGATCGTTAACTTCGCTATTTACTTGATCAAACTGCTCAACTGTTGGTTGAGTTTCAAATGAAGAAGAAAAAGTTGTTTGGTCTTGTTGATCTGTATCAATTGGTAATTGGTAACTATCAACTACTTGGGCTTGTTTACTTTCAAAATCAGTAGTTACTTTTTTTTCAGCAGTTGGTTGTATTTCCTCATTATTATCAAGATTAGCTTGAACTTGATCAAATCCAACTTGATAATTATTTTGTTCTTCAGCAACTGGTTTGAGCTCTTCATGGTGATCAAAGTTGTACTGAACATGATCATCTAAAGAAGGTTGGTCAAGGGTTGGTTGAGGTAAATCACTGTCAAAGCTGTAGCTATCAGTTGTTGATTCAGGTTGTTTTGCAAAATGATCATCTGAAGAAGGTTGGTCAACAACTGATTCAGGTTGTTTTACTTCTGGTGGTAAATCGTTATCAAAACCATAGTTATCTTCAACTGATGTTGGTTGTTTTGCAAAATAATCATCTGAAGAAGGCTGGTCAACAACTGATTCAGGTTGTTTTACTTCTGGTGGTAAATCGTTATCAAACCATAGTTATCTTCAACTGATTCAGGTTGTTTTACTTCTGGTGGTAAATCGTTATCAAAACCATAGTTATCTTCAACTGATTCAGGTTGTTTTACTTCTGGTGGTAAATCACTGTCAAAACCATAGTTTTCATCAGTTGGTTGAGATTCTTTAGTGGAGATCCACTGGTCATTTTCAAAGTAACCAGATCAAACCCACTCACCATTTTCATCATAAGAACCATAGTCAGGGTTACCAACATACTTTAAATAATCATTCTCTTCTTGCTTTTCATCAAAAACAAACGGATTCCCATTTTCATCAAAGAGTTGGGAGATATCATATTCAGTATCTCCATAAGGATCATAGTAAACCCCTGTTTCTTTGTTAAAAGCTATATAATAGCCATTACCATCAGCATCATATGCTACCTTAATATTAGGGTCTTCTTCAACAGTGGATTTACCATCATAAAGATCACCAAAAATGTTTTCAGGTTCGGTTTGGGTATAGTTTTTTTCTTCAAAAACCGATTGCTTGTTTTTAGCCATCAAGAAAAGTTATTTATGCCAGTTTAGTCAAGCTTGATTATAAAACTTATCGCTTCGCTCTAACTCTTCCATGAAAGCGTAAATAGTAATAAATAATTTTTTAAATTATTTTCCACAATCCCTATTAAAGGGTAAAACAAAGCTACATAGATAGGTAATAATAACGCTTGCACCAATACTCTTGGTAAAAGAAAGAAATAAACCCCATACAAAACAAAGTTATTACTTGGTCTGCCATTATTAATAAAAGTAATAAACTCAATGGCAGCAATTGTCCCCAAAAGGATAGAAACAACTACTGACATTAAAACCATTAGAACCAAGCTATAAATAGTTAAAAGTGCTTGTTCTTTATTTCTCTTTTTTACATAAACAAACCCTAAAAATCCTTCTGTTACTAAAAAGAAAATAACAAAGCTAACTAAAGTTATGATTTGTAAAACAACAACATTAACACTAAGATCAGCAGTTTGAAAATTTTTTGTTCCAATTTGCTGAAACTCATTATTAGTTAGATAAAGTAAAAGCAGTGTTACACTAACAACAAAAAAAGTTACTAATAGTGTTTGTAACACCCAAAAATCAACCTTGTAATTACTGGCATTTTTTCTAACTAGATAAATCCCTTTAACTATGCCAGCAAGAAAAGCAAAGAGTGGCTTTTGCAATGCAAACATCCAAAACCACACATAACCCTTTGTTAACCAATCAATAGTATCAGATAAAAATCCAAAAATAAAACCCCAAATAGGACCAAAGATCCATCCGAACAATGCAAAGGGAATCCTTAGAAAACTAATGCTTAATACATTAGTAACACTAATTGAAAAGATAGAAAAGATAAAGGTTAGTGCTAATAAAACACTAGCTCAAACTAACAGTTGTAAACTCTTTAAAAGTTTCAACGGATAGTAAGGAAACAAAAAGTTTCAAAACAGTTTTTTAGATTGAAGCTTGAATGTGAATTTTAACCAAAAATAGTAGGTGGGGGGATATCTAAAGTAGTTTTACTTTCATCACTAGTAATTGGTTTTGCCAATTCCTCTTGAAAGTACTTATTAAATATCTCATTAGTTTGTTCTAAGTTTAATTTAGGACTTAATGAAAAAGTAATTACACTAATGATGCTAAAAGGAACAATGCTAAAAACAAACAATCAGGCGATTCCTATCAGATAACTTGCAAATAATGGTGAAACACTAATTAGTTGGTTTTCACTAAATAAGCGGATGATTAAAGCTGCATTTCAAAAGCCACTTCTTACTATATTACCAGGGATACAATTTAAAAAGACAAAGATAAAGATAGTCCAAAAGATCCATTTTATTCTTACCTTATAACGGTAGTTATCATTAATTTTGGGAAACTCTTTGACCCCAAATTCACTACGTAAATACCGCATTGCTCTATTGGTTTGACCTCTAGTTGCAAAGCTATAGGGTTTACCCTGAAAAGCAAAGAAAAAAGAAGCACCACCAAAAGCCAGGAAGATCATGGTAATCAAGTTAGATGAACTTAAAGCAAAAAAGCGGTTTAGATTTGTTGTATCTGCATTACTTTGCTGAACAGTATTTTGGTCATTATTAACATTGAAAAGTGGACTGTTCTTATCAACAAAGTGGGTAAAAAAGACATTGACCATCACAACGTATGCTATCGAAATGAAAAAGATGGCAATCATAGAGATGAAAATCGCTAGTTGTGCTCATTTAATCCACTGACTCTTAAACTGAAACTCAAAGATCTTACCAACTAGTTTTTCAGAGGTAAACCTACCAAAGGCACGATCTCTGGCACGAACTTGGAGGGTTTGTTGCAAAATATTATTCTCAAGTCCTGAGTTTAGCTGTTGAGAATTGTTTATAGTTTGAAAACTTGGGTTATCTTTAACTGTTTTATTGTTTTCTTGTAACTCACTAACGGGTTTAACTTGAGTTTCAGCAAGTTTGGTAAAGCTATTCAACTCTTTATTAAACAAGTTTTTAACAGCAACATTAAGATAATTAGCTAAATCTAACGCATCAGTTAGATCAAACTTAGCAGCTTTAAGTTCTTTTTCACTTAATTGGTTTTGCCCTAATTGGATAAACTTATCATGAAAACTACCAAAAAGAAAGTGGTCTGGTTTTTTTGTTTTAACTCTTTTTTGTTTTTCCTCTAAAAAATAAAGTAATTTGAGTTGTGCCAAAATTGTTTTGGCAAAATCACTATCAGTTTTTATCCTTGTTCTTAAAGTTTCGTTATTAAAAACAATGTCAAGATTATTTTGTACCTTAGTTAGAATTTCACTAAACGTCATTTTGATAAGTAATTTTAGAGAGGTTAGTTAGTAAAAAAGCTTAAAGCAAAGTTTTTTAACAACGGAATGGCAAAGCCATCATTGTGCTTTAATTTAATATCCAATTTTTTGAATTGCATTAATTATATTTACTAAAACTTTGCTAGTAAAGTTATGGTACTGACTTTGAATAGCAATTAAGCGAAACTGATTAACATTTAGATTAATGTTAGTTAGTTGCTTTGGATTACAACTTTTAACCATCAATGCTAATAACAACTCCCCACTAAAAAATTCTAAAAATTGGATGGCAAAATTTGGTTTTGTACTTGCCAATTCATCAATATATTTAAATGCCTTTACAATTTGTGAATTTGTTAGAGCTTTGGTTAATTTGTAAATTTGAACAGGTTGATAATCACAAATAACTGTTTCAACTAATTTGTTATCTTTGATTTCATTTTTACCTAACAAGCTAAGTTTGTTAATTTCTTGGTAAATTACCCCCATATTTAAAGGTAAAGCATTCGCTAATCAATCAATTATTTCCAAATCAAGTTTTAAGTTAAGTTCTCTACAAACTTCACCAATTGCTTTATGCATTGATTTTCAATCCAATTTATCACAAAAAACAGTTGTGATTGATTTAATGGTTTTAATCCCACTAAAAGGATTGTCAGTATAAACAGTTAAAACTACATCAGTTGTTTTTAGTTTTTCTAAACACAAGTTTTCTTGTCTAGTTAAGCTTGTTTTTTCCAAAAAACTGCAATTATTGACAATAAACTTTTCATTATTAGAACCAAATAATGGTTGTGAAAAGAGATCATAAAGTTGCTTAAGATCTTTAAACCAAATAGTTTTATATGGAGAATTGCTTTTGATTTGACTTAATTTTTGGTGAATTAGCCCAATATCTTGACCATAAATTATTGTCATAATAAGCTACTGTAAAAATAGTATATAAAAGTCCCAATAAGCCATAATAAACAAAAAAGAAAATCAGATTAGGTTTAGGTATTTGTAGAAAAACTGTTACTTTTAATGATCATTCACTTAGCATTTTAAGTGGGAGATAAATACCAAAACTAGCTTGACCAAAAACACCAATAAAGGGTAAAATAATTCAACTAACACAAAAATAAAATAAGGCAATTGGGCTAAACAAAAGGTTAAACAAGACACTAAAAGCATTTAGTCTTGAATTTAAATACAAGCTTAAGGGGCTGATAACAATCAAGATTAAACTAGAACTGACAAGCGGTTTTAAAGCTTTTAATAACTTTAGTTTGTTGACAAATAACAATACAAAACAAGCTAGAAAAGAAAAATTAAAACCAAAGTTATTTAAAGCATGGTTACTAATAAGAATGATTAAAAGTGCAGTTAAACTTAAGTTATCTTCTGGTAACTGTTTTTTAAAAACCTGTTTTAACAGTGTTGAGATAAAAACTCTTAGTGCTGAAAAAGCAAATCCTACTAAAAACAGGTAAATCAATAAAACAGCAAAACCACTAAGTTTGTTTAAGTACCATCTTTTTCATAAAAACCGTTCCATTAAATTAAACAAGAAACTTAAGTGAAAACCACTGATAACAAACAAATGGACAATGTTTAGTTTTAAAGCATTTTGGTACAAATTGTTTTTAGTGGTTTCATTAATTAAAAATAGTTTTAAATACTGATTTAACTCACCTTTAGTGGTCTGATCAATTCAATTAATGAAAGGGGTACGAATAAAGTTATTTTTAGCAATTTGATTTATCTTGACTGCTGGAACAAAGTAACAAAAAGCAATAAAAAAGCCAACAATTGTTAAGTTCAATAAACAAAACTTTCAGTCATACCAAAGACTAAAAAGCGTTATCAAAATGATCCAAATTGACAGTGCAACAAACTGTTCTTGTTGTACTAAAAAGAAGCCTGGGATAAAACTGAGTAAAACAAAAAAATAAAACAGCTTTGTTTGCATGCTGTTTTATTAATGAATTTATTTTGAACTTTTTAACAAATTAAAAGTCCTTAGATCAATATGATCTTGATGGATAACGTCTAAGTCCATCATAGCTAGAACTAAATGGTAAACTAGTTCTTCTAAAACTTGATAGTCTTGTTGGTGAATGGAATGATGAACGGTATGAACTGATATTAGTTAATCTTAATGGTCTTCTTGTTAAGGGATAATAACTACTTGGTGTTCTGCTAAAACGATAGTTAGAAATAGCGTTATTTTGGAAATTAGTTGGTCTTAATGGTGTGTAATTGTTGACTCTAGATTTTGGTGTGTAGTCAAAATCAAGAGAGAAACGATCACTAGTTAAACTTTGATTGGCATCACTGTAAAGTGGATCGCTGTATTTAGTTGCAACATACTCATTTTCAGGATAAAAACGGCTGAAATTGCTACTTTTTATAGTGTTATCACTCTTTAGAGCAATATCATTCATTATCCTTTGGTGGTTTAAAGCATTAATTTTCTCTAGAAGAGAAGCGTGGGAAGAGACATTAACAGTGATATCTGAATCAGTTGTGCGTTCAACTTGTCTAGGTTGAAAACCAGTGTCTAATTTTGATTGAAGTGAAGCTGCTGATCTTGGTACAACTTTAATTTGTAAACCTGAAGTTGGCAATGGTTGTTGGGGTTGATATTCAGTTTTTACTTCTGGTTTTCAGTGGTGAACTTCAAATTGAGGAGTTGATTCTACTTGAGGGATGTATTTTGTTTCAGGTTTTGGTTCAATTCTTGGTTCAACCATTTGAGGTTTTAGTTGAACTTCAACTTGAGGAATATGTTTTATTACTTCTGGTTTTGATTCAATTCTTGGTTCAATCCTTTGAGGTTTTAAATCAACAACAGGTTTTGGTTGAACTTCAACATGTGGTACATGTTTTACTTCAGGTTGTTTAACTTCTGGTACTGAATCAACAACAGGTTTCACTTCTGGTTTTGGTTGAATTTCAACATGTGATACATGTTTCACTTCTGGTTTTGGTTCAATTTTGGGTTCAACTATTTGAGGTTTCGGTTGAACTTCAACTTGAGGAATATGTTTTACTTCAGGTTGAACTAACTTTGCTGGTTTATTAACTAGTGGTGGTTTTAAAGAAGGGGAATTATTAAAACTATGGTTTTGAGATCCACTATCAACTTGAACGGGTTGTTGGTGGACTAAATCCTGATGGAGCTGTTGAGTAGGTGGCACTTCTTTTTCAACAAACTGGTCATCACCCAAGTTTTCTGTAGGTTGTTGGGGTTGATTTAAACTGTCATTTAATTCAAAGACATCAGGTTCACTTTCAGTTTCTAGAGTAGTGTGTTGAGCTGCTTTTGGTTGGTCATCAACTTCTATCTTAGGAAGACCTGCATCAATACTAGGATAATCTTGTTGGGTTGGTTGTTCTTGAGAAGTTTGAGTACTGCTTAAATAGTTAATTAAAGGGACCTTTTTCTCATCAGTATAGCTAGAAACATCCAAAACATTACCATTTTGGTCATAGAAAGTGTTGTTATCCTGATCATATTGAACAGTTACCAAGTTGTTCTTATCATCATAAGCATTTAAGCTAACAATGTTATTTGTAAAGCTAATAGGGTTATCTGAAGTTAATTCAAACAAAGGATAGTTACTAGCTAACTGCTTTCTTTTTTCTAAAGAGAAGATATTACCTGATTCAGGATCTTGGTATAGAGCATGAACCTTATTTTTATGATCACGGGCATAAACAACACTAACATCAGGATATCTTTTGTTAATCTTTTTTAAAAGCTTGTTATAGGCTTTGTTTATTTTAGCTTTCTGTTTATCGTTCATAAAAAATTACTAAATTAGGGTTTTAAACCGCCTTTTGGCATCAATTGTGATGGGTTAGAACGTCGTTGCATATTTGGATTAAACCCAGGGCGTTGTTGCATTTGGGGATTAAAGTTATTTGGTTGTGCAAATTGGTTAGGATGGGGTTGGTTAAAACCAGGGCGTTGCATGTTTGGGTTCATCGTTGGGTTGAAATTATTGTGTGGTTGGAACTGTTGGTTTGGTTGGTTAAAACCAGGGCGTTGTTGCATGTTTGGATTAAACCCAGGGCGTTGCATGTTTGGGTTCATCCTTGGGTTGAAATTATTGTGTGGTTGGAACTGTTGGTTTGGTTGGTTAAAACCAGGGCGTTGTTGCATGTTTGGATTAAATGCAGGTCTTAAAAGCGGAACACGCTGTTGAAACTGTGGTTGAAGGGGTTGTTGAACTTGGGGTTGGGAAGGCTGAGCTTGGGATTGTTGAGGATCAATTTCAACTGTTTGTTGTTCTTGTTGATCTGAGATTCTTTGTAATTGCTCAGCAATCTGTTCTTGGCGTTCCTTCTCTTCAAGTAAACGTTTTTCTTTGCGCTTTACAATTGGTAAACCTATCGCAAGTCCAAGGATAATAGCTAAAGCACTAAAACCAAATGAACCTGCTACTGTTGGGATAAATCAGGGCTGTTCAACAATTGATCTGCCCTGAATTACAGTTGTATTATCTTCTGTTTGATGAAAAACAACCCCTAGTTCACTACCATTAGTTTTCACAACAAGTGCAAATGCTAGTAACGATAAACTAACAATTAGGATTGTTGTAAAAAGTAAAGCAAGAACTATAAAAAGTTTTTTGTATCTCAAAAATCCATTTAACTCCATCTTTATCAAAACTTAATTAGTTCCTTTTTCTTCTTGATCTTGAATCATCATAATACTCTTCTTGTTCTTGCTTTTTCATGTTCCTTTTTCTTCTTTTAATTGAACGAGTGATTTTATTGAGAAAGAAGTTAACAATTAAAGTACCTATTAAACCACTAACAAATGCCATTCCTAGGGGAATTACATACTTAGTTAGATAAGGTGTAAACTGTTCAACAAATCCAGGTAACTTAGCATTTGTCAAAAAATCTACATATCCTGGGATAGCATAAAATGCACTTTTTTCAGTTGGAAAACGGTTATCCAAACCATTACTTGAACCTTCTAAAAGTTGTAATAAGCTACTTGTTTGAGCAGTTGCAGTTGCACCATTGTTTGAAGAGATGTTATCCAACGCTTCTTTTGAAAAGAACCTGTTGAAAACATCCATTGGTTGTGCTAACTCAAATGGGTTTAGAGAACCAAAGTAAAAGATAGCTAAGAAAAGATAAGCAAAGCCTACCAATACCAATAAAAGAAAGCAAAGCTTAAAGAGAAATCGAAACAAGCGCATTTGTATTAGTCAAAAATAAAACTACTCTGTGAATTTTAACTTTATTTAGTTAAGCAAATGGTTTATGTAATTAATTAAATTAACTATAGAGCTTTAGAAAGCTCTAAACTCTAGAAAAATTTTTAGTAATTAGGATGATTCTTAGGATGAAATAGACCAAATTCATGAAGCTGCTAATTAATCAAGCCCCAAAGAATAAAGCAGATCTTCAGCTCTGTCTTCTTGCCATTTCATAATCTTCACTTTCATAGTATTTAATCAAATCTTGTTCATTACGGATCTGTAAACTCACAAAATAAACTGAAATTAATGAAAAGATTCCCCCTACTATTAAAGATAAGCCAGGGATAATTCTATCAAGTAGGTTTGTTGTAGAAGCAAAGACTGTAAAGTTCAAAATAAAGCTCAATAAGCTCGCTACAATACTAATTACAAAAGCCCATAATAAGATGTGAAACAAAGTTTGGGTTTGTTGATATCGCCTTTTTTGACTTGGTAGCGCACTTAATCCTCACACTGAACCAAACACCAAACTAGCACCTAAAAAAGCATAAACAATGTCATTAGCTTCAAAGTTAACTTGTTTTGAAAATGTTGTTGCTAAAGTTAATAAGATCCCAAGGGTTATCCCATAACTAATCACATCAGTAATGATTAAGGCCCAAAACCATGTCAAACTAGTGTTGGGATTCCTTAATTTAAAAGCAGTAACAAAATAAAGAATTAAAGAGACAAAACTAGTAATAACTGCAATAGTACTAAGTGTTCTAAAATCACTAGCTAAATCAATTAGTGTGCTTCTTGAAAGCAATTGTTGTACTGTTAATGCAACAAGAAAGATAAAAAAGATCCCAAAAGCAGCTACCATAAAAGTTAAACGGATTACAGAAAGCTGTTTTGTTTGTACAAACTGCTTTGCTGTTAAACCGTTTTGATCAATATAGCCTTTTGTTGAATTAATCATGACAATTTGACTTGTTAAATTTTAATTTGTCAATATAAAAAAACTAATAATTGCAAAATATATAGATAAGGATACTTACCCAAGTGGCTGAAGGGGTAGGCTTGGAAAGCTTATAGATGGGTAAAACCATGCGAGGGTTCGAATCCCTCAGTATCCGCCAGGGAGATTTACCCAAGTGGCTGAAGGGGGCGCTCTCGAAAAGCGTTAGGTGGTTATCCACGCGTGGGTTCAAATCCCACAATCTCCGCCAAATTCTTAACTGTATAAACAAAAAACACACCATTTTGGTGTGTTTTTTATGTTCATAAGTTAGTTGAATTTACTCATCAATGTAACCTGAATCACGCATTGTAGCAATCCCTGAAGGAGCATTACGGAGTGAAAATCATGGTTTTAAAAGCGAAGGAATAGTTTCATTTAACCCAGGAGCGGAGACTGGTAGAACAGGAAAGTAACTATCTACTACAGTAATTAAGAACTTAGCATTATCAGTGTCCTGTTCAGCTCAAATGCTGTTAAAGCGATTAAGTTGAGCAGCGTTGTCATAAAGATCTTTCAATTCATAACCAATGTGGATATCTGATACATTTGTTGGTTTGTTGTCATCTATCTTGCTTCTTTTTGATAGATATTTATCAGGATCTTTAATTGCTTCTTTAACTTTATCAGCTTTCAATACCTTATTATCAACTAAAAATTTCAGTAAGCTTAATGCTTTTTTAGTGAATGCTAATGAAGCGGATGTACCGTTATTACCATTAGTACCAAAGTTCTTAATGACATAGGGATCATCTTTAAAAAGATCAGCACTTGTTATTCAACTACACCAGTCTTTTCCGTTAGTTGTTGTGTTATTTTGAAGTTTATTTTCAATTTGATTAAAGACATTGTTTTGGGATGCAGCTAACAATAAAGTTCTGACAATATGACTACCTGAATTGTAAGTAATTACTTCACCAATACCCTCACTGTTAAGCTGTGTAGCAGCATCAAGTCAGGTACCAACCCCATCATAATCAGGACTCCAATAAGAAAAACCAAGTACACTTGATAAACCTATGTATCATTCGTTTCTTGTTGGACCTTGTGCATCTCCTAAGATAATCTCAGGGATAATTAAATTTTCACCATTATCATTACCAAGTTCAGTTAAAGCTTGTTTTAAAGCGTTATAGTAATCAACTTGGTTAGAGGAGAGTGATGCATTTCCTAGTTTAATGTTGAAGTGTAATGGATTGTTACCAGTAAACCCAACTTTTTTTAATGCACTGATAAAACTTTGCTTGGTAACACTTACTTGTTTTGAAGAAACTTGACTAGTTTGAGTATTGTTACTAGTAGAAGCTGTTCTTACTTGAGCACCATTAGTTTTTGTAGAGCTATAGTAACTTAACTTAACAGTTGCAGTATCAAGAACATTCTTATCCTTTTGAAAAGTTCCAGTTTGTTCAGGGATTAAACCAAGTCTTTGGTTGTTGTTAATCTTGTAATAATAATCATTCTCAATTCCATCTGTATTTGTACCACTACTAGTTCCATTTGCTGGTTTTTCTTTAAAGATCCCAAAAGGAACAGTAGAATAGTTAACATCTCCTGAGTTGGGTAGATCAATGATTGCTAAGTTCTTTCAGTTGATCAAACCATTAATTCCAGCACGGATAGTCAACGCTTCTTCAGATTCACTGTTATATAAGAACTTAGCTTCTGTCTCAGTGATGTTTGCATTTAGTTGAACAGTACTGTCATTAGCAACATAAGGTTTACCTGAATAAGTTACTTGAGCACTTTGCGCAGTTTTTTGGATTTTTAATCACCTCAGATCCCCTGTACCATTAAACCTTGATCTTGCATCTTGTTGTTGGGAATAGGGAACAGCTGAAGCTGATAAGCCCCCAGCGATATAACTAGAATAGAACACTTCAGGAGTAGCTCCTGGTTGGAAATAACTAACTATAGCAGGGATAGGTTTTTCGTTTTCTTGTCTTGTTTTAGGAAGATTAGGGGTGATAATTGCATCATAAATCTGGTTGCGTTTAAAGACAATTTGTGCTTGGTTAAAACTCTCAACATAGTAAGGGCCAACTGATCAAGTGTCTTTTCAAGCATTAACACCCCCTCCATAGATACCATCAAAGTTAGTATTTGCTTGATCTAAAATCTTTCTGTTGTTATTTTGGTTATATTTAAGCGGTGAATCTTTACCTAGTTTAAGGGCTTTTACCTTAGGATGGGTGTGAGGAATTGGAAAGAAGAATTCCTTTGACATCATTGATAGAAAGAAAGGAAAAGGAGAAGTTAGATAAACATTAAACTTGTTATCATCTACACTTCTATAGTTTTCAACATCATAATTTTCATCAGTGATCTCAATACCTTTACCATTGTTACCACTAGAACCGTTGGTTTTATCCATACCAACGGTTTTTTCAACATCAAGACCCATTAGGTCAATAAAATAACCATTCCGGTTAAAACCTAAGTTAGAAGAGAGAATATAGGTCTCAAAACCCCTTTCAAAGTCTTTAGAGGAGAGTTTAACTGGTTGGTTGTTTTGTTTGACTTCTCTACCAGCATTATCAACCCAGTTCAAACTAGTGTTAATGGTAAATTCATATCTGGTTGCTTCTTGGGTTATCTTGTGATAGTTAGGATCTTCAGTAATAGACTTTGAACTATCACGATAAATTGTGCCTATTGCATCCCACTCATTGAACTTAGTGTAATCATTCTTACTAGATCCATTAGAAGAACCTGTTGAACCATTTTTTTGTGCTCTACCTTTAAGACCACTGTGTGTTGTCTTTTTATAGCCAGTAACTGATAAAGCAAGTTCTAACACTAATTTTTCTTTAATTTGGTCAGTAACATTAACCCCATCATCACGGTTTGTACCAGTAGCAACATAGTTAGTTAACCCTGCATAAGCATTACCAAAAAAAGATGAAGTGGGGGAAACGTTAAACATGCTCAATGGAACAGAGTTGTTCTTTAACAAATCTGCTGAAGAAGAGGTGAAAAACACCCCTTTGTTTAACTGTGAGGATGCACAAGCACTTAGGATTAGTGCTGCACTAACTGTCAGTGTAGAACCGAGCAACAAGTATCTTTTTTTTAATTTCATTGTGTTCTTTCATAAATAAATCGGTTAATTGATTGCTGATTAGTAAAAAAGAGTTAAAAAACAGCAATTTACCCTTAGTTAAAAACTAAAAGTAGTTTTATTCTAGTTTGCAATCACATTAGATTGCAAAATCAAAAACTAATGTTTTTATCAATTGCTCACTTTTTAAACAAGAAAATGCTTAATACAAAGTTAGCAAAACTAAAAACATGGTGCACCCGAAGGGACTTGAACCCCCACTTCTAGATGAAACTAGATCCTAAGTCTAGCGCGTCTACCATTCCGCCACGAGTGCATTATTGGTGCATCTTGAGGGGATCGAACCCACGACCCAATGATTAAGAGTCATTTGCTCTACCAACTGAGCTAAAGATGCAGATAGTGGTGCCGACTATAGGATTTGAACCTACGACCTATTGATTACAAGTCAATTGCTCTACCAACTGAGCTAAGTCGGCATGGTGGATTGTGAGGGGATCGAACCCCCGACCCTATGCTTGTAAGGCATATGCTCTCCCAGCTGAGCTAACAATCCATCACTTATGGTGCCGAATACAGGAATTGAACCTGTAGCCTATGCATTACGAGTGCATCGCTCTGCCCTTGAGCTAATTCGGCATTGGTGACGCGTACGGGATTCAAACCCGTGAATGCACGCGTGAAAGGCGTGTGTGTTAAGTCTCTTCACCAACGCGCCAAATAATGGCGGCCACAACAGGGATCGAACCTGTGACCAACCGGTTAACAGCCGGTTGCTCTACCGCTGAGCTATGTGGCCTGAGAATAAATTCTAGAATTTCTAGCCAATTTTAACAGCTTCCTTAGGATATGAATAACTATTTGCTTTGGGATGTTTTCTAACAAAAGTTAGCACAGAACTGGACACACCAACCTGTCCCATAACCATTAATAGACATAAAGCAAGGAAATTAAAGGTATTTCTATTTGGATCTAAAGCAATGTTAACAGTAGCTCCACTTGAAAGTCCAACTGTTCCAAAAGCACTAGTTGTTTCAAATAAAGCATCAATGAAACTAACTGGTTGTTCCATACTTAAAGGTAGAAGAACAGCTGTTAGTAAAACTGCAATTAAACTTAAAATTAGTACTAAAAAAGCATCTATTACTGTAGTTTGATCGATTGAACGCTTAAATGCTTTTACTTCCTTTTGACCTTTAAACTTAGCAACTAGAGCTAAAAAGATTACTGCTAGCGTAGTTGTTCTAATCCCCCCAGCAGTAGAAGAGGGACTAGCACCAATAAACATTGCCAATGCAATAATTATTTTTGTAGTTTGAATCTCACTAGCAACAGGGAAAACACTAAAACCTGCTGAACGGGATGAAATAACCATAAAAAAGAGCTGCATTACCCTACTTGCATTAGGGTTATTACCAAAAACTGACGCATGGATTGCTTGGTTAGATTGTGATTGCAATTGGGTATTTATTAAACTCTTTTTTTCATCACTAAAATTAACAATAGTGTTAGTTAAACTATCACTAGCAATAAATTCCACCATTAAAAGTAAGGTGAAAAAAAGCAGGATTACAACGATATTAGTAATTACTGTTAACTTGGTAAATAGACTAAATTGGTGTTTTGTGTGTCTACCATACTTAATCTTTTTTTTAATGGCTTCAAAGCCATCAAACAAACAAGGATAACCAATTCCCCCAAAGATAATTTGGCTAATAGTTAACCACTGGATAATAATACCAAGTCCATTTCTATAAGGAACAAAAGAACTACCCCCTATCAGATCTATCCCAGCATTATTAACTGCTGATAAGGAATGGAAAAAACCTGCTTGAAAAGCCTTATTAATATCATTAAAAGCTGCTATTGTTTGGTTTGAATCAACTACTAAAGCTTTTAATTGAGTTGAAACTTTTGCATGATCTGCAAACAAGTTAGCAGGTTCAAAGCCTGGGATGAAATAAAACAAAATACCATATAAAAATCCATAAATTAGTTCAACGATAAACAGAAAGATGATAGATACTAAGATCATCTCACTAGTATTACCTAGCTTAGAACCACCTCGTTCTGATTGCAACATTAACTTTTCATAAAAACTGTATTGTTCCTTCTTGTGAAAGTTAAACAATCGTCATGTTAAAAAAGCAATAACAACAAACCCAATCCCTCCTAACTGGAGTAATACTGCTAAAACTATCTGGCCAAAGATACTGTATGTTTTTGATACAACAACAGTAGAAAGTCCTGTATCACTAAAAGCACTGGTTGATAAAAATAATGCATCTAAAAAGTTGTAATCGGTTTTCTGTTCAAATCTTTTTCCCTGTCAATCAATCCCATAACTAACCACTTTTTGGTAGTTATCTTGGAGTGCAATTGGCAAAAATAACAGCAAACTTCCAAACAAGATGCAATAGATATAAAAACAAAAAATTCGTTGAGTAATAGTCTCACCTCAGCCAATTTTTTTTAACCAAGTGGTGAGTTTTGCCATCTGGTTTTGTCTTCTTTTCATTAAAAGAACGCTATCAAATATAATTTTACCAAGGTATGAAACGTGCAGATTTTTGCATTATTGGTTTAGGTAGATTTGGGATGCAGGTTGCACAATCACTAAAAGAAAACAACTTTAACTTACTTTTAATTGATCTTGATGATAAAAAAACTGACACCGCCTCACAACAGTTTGATTATGTTATTTGTTGTGATGCTAGTAATCTAACTGCTTTAGAAGAGTTACAAATTGATGAGTTTGCTGGGGTTATTGTTGGGGTTACCAACATAGAAGCGAGCATCATGATATGTGCTAATTTAAGGGAATTAGGACAAAAAAACATTATTGCTAAAGCCAAAAATGAAGTGCATAAAAGGGTGTTAAGCACAATGGGAATTAGAGAAGCTTTGATCCCTGAAAAGATTGTTGGTAAAAATCTGGTTATCCGCTTAATCCATGGGCTTGAAAATGAAATTATCAACCTTGGTAATGAGATAATTTTCATCCGTTCAGCAGTTAACAACAAGGCTTTTTTTAACAAAAGGTTAGAAGAGATTAACTTTAGGCAAAACACCGATGCTAACATTATCTCCATCATGCGCAGTAATAAAACTGTTGTTTTTCCTTTAGGACCAAATACTGAGATCCAACCAGGGGATATTATCACTGCAGTTTGTCAACAAAAAAGTTTAAATAAGTACTTAAATTACATCAATCCTAAAACCAAAAATAAAAATTAAAAAAAGGATCATCATGATCCTTTTTTTAGATAGCATGCTGTCAATAAGCCATGTTCTGTTTTAAAGTCAGTTATTTATCTACAGCAATTGCTGTCATTACTTTAATTATTTGTTCTTAAAGCAACATTCCCTTACCAAAATTTAGGTTTCTTGCTTGTGGAGTTTACCGCGTTTCATACCTGGTTTTCACCAAGCTCGTCTCTGTGGCACTTTCAAAACATCATCATAGTATTAACCTTAGACTAGTTATGTCGTTATGGCTATCACATCCTAAATCTTATCGCTTTGATTTACACAAACACTACTTGCATTCCAGCAAGTGCAAGCATGGACTTTCCTCTACTTTAAATATATCTTTAAAGCAGCAACTAACCGACAGCGTCAAAATTATAATTTGGTGTTGGGGATGTTTTGGGTTTGACATAATGCTGATAGACAAACAGTAGCATTGGGGTATGCCCCTTACAGCGCTAGGTTCAATAACCGACAAAGAAAATAACGAAGTGTTGGTAGATCCAAATTTGATCATTAACCAACAAGCAAGTGTTAACTTTGCTTTTGCATAAGTAGATACTAAAGCTACAGCTGGTGAATAGTCATAGTTTGCTAGCTGTCATAGTTTATGACTCGAGGTTAAATCGTTCAATTTAACCTTTAAAAATAGAACTTGTTGTTTCCATGATTGTTTTGTGATCAATTGGAAACAAGACAAAAATCCACAAAACTAAAATGTAGAAGCTGTTTGTTGTGTCCTTTATGGAAACGGGTTCGATTCCCGTCATCTCCACCATTTATCAACTTTAAGTTGGCCTTACACTCCCCTAGTTGGGAGTTTTTATTTTGCCTTACTCTTTTTAAAGAGTTGTTTTAATTTGGTTTTGTTGTAATTAAAAGCAAAGATAGCTATAAAAAAGAAAGAATAAAAAACTAAGCTAATCGCTCAAGCGGTAGCAAATACACCAAAACTATTTAATTTAGAGAGATAAACACCATTGTTATTTTCAACATTAAAAAACAAGCTAAACCGCGTTGCTGATTGAATTTTTTGTGTATCTCTTTCAAAAAACTGTTGGTCTAATAACACCAAAAATAAAACCATTAGCAAGATCCATAAGAGATAAACAACAATACTGATAAAAAATCCAGTAGTATAGCCAATGCGCTCTCACTTAGTAGTCTGACTTTTAAAAAATAATGAAACAGTTCATCTAAAACTTAGAAAAACTAATAATGCTAAACTAATTAACCCAAAAACTAAGTTAGCAATTCCATATTGACTAATCTGATAAGTAATACTAGCTTGAATAATATCAGTTGGTTTTTCAACAGTTCAAAAAAGGTAAAACTGATTAAAGCCGTTAACTTCGTTAAAAGTGAATTGTTTAGTGTGAAAAACGCCAGCTAAAAACAAAACGTTGTTTAGAAAAAAGCAGAGAAAAAATAAAAAGTAAACAAAAGTATCAAGTAAGCCAAACCTGCTGTAAAACAGATAGTTACTGTGTTTGGAAACAACTTGATACTCAGCGACATTTATTTCACTGAGTTTATCAGATATCTTATTAATCCAACCACCACCACTATTTTTCTTTTTAGAAAGCACGTGGGATAGATTTAGATAATCAAACTGATTTTTCTTTTTTAACAAGTACCATATCTTCTATCCTAATTCCACCAAGGTTAGGAATATAGATCCCAGGTTCAATGGTTACAACCCCATTTTCACACAATAACTTGTTGTAAGATTGGGAAACATTTGGCATTTCATGGATATCAATACCAACGCCATGACCAGTACTATGCACAAAAAAGTCTTTAAACTCAGAGTTTTCAATAATATCGCGGCACACCTTATCAACTTGTGAACCTGTTAAAGTAGTGTTTACTGCATTTATACCAGCCAAATTAGCCTCTTCAACTTTTTTGTATGCAGATAATAACTTTGCACTTTTAGGTTTTTTACCAACTAAAAAGGTTCTTGTAATATCAGAGCAATAACCGTTGTAAATGGTGCCAAAATCACAAGTGATAAAATCACCTTCCTTAACTATTGTTTTGGTTGGTTTGTGATGTGGGTTAGCACCGTTTTTACCAGTAGCTACTATAGGATCAAATGAGTTTTTAGCCCCACCCTGCTTCACAAGCTCATTAGTAATCCATTGTGAAATGAAAAGCTCAGTCATTTTTGGTTTAATAAAACGCTTTAACTTAACTGCTACTTTTCTTGTAATATCAACTGCTTTTTCAATGGCTTGAATCTCACTAGGCAACTTTACTCTTCTAATCTCTTGGGCATTAATTACTGTGTATTGTTTACAAATAGCTTGAATTCAATCCTGATAGTTAAAGGTAAGATAGTCACCCTCAATTAAAAAGTGATTGATACCATTAGATTCACAAAAAGCTTTAACTTGTTTAAAACTAACAAATAATTCAACTTCAACAATAGGATTAATAAAGTTCCTTGCTGCTTCATAATACCTACCATCAATAAACAATTTTGCTTTATTGCTGGTGATGATTAACCACCCTGCACTACTTGGAAAATTAGTTAATCAAAAGCGGTTTTGATCAGAACCAATTAGGATAGCATCAGCTTTATTGGTTTTTAAAAGATCTTTAAGAACTGTAATTTTTTGTTGTAATTCACTAATCATTATTTACGTTTTACCTCTTTGTGCAATACTACCTTACGACAACGTGAACAAAACTTATTAAGTGCTAGTTTTTCTGGATTTTTCTTGACGTTTTTAAAGGTTAAATAATTAATCTCAGAACATTCATTACAACCTAGTCGTGTGCTTCTTTTAACAGCCATAATTAAATTATCTTAATTATTTATTGTCAATAAGTAAACTAATAGAAAAAGCATCAATGCCAGTGTGAACAATAATCACACTTGTAATGAAAGCATTTTCAATTTCATTTTGAAAGTTAATCTTATTATGCTCAATGAAATCAGTAATTATTTTTTTAATTTCATTAACGTAATTTTTACAAAAAGAATAGCAAAAACCGATCCGTTTAATTTTGTAGTTATCACCAAACTTAGTTTTAACAAAACCAAATATCTTCTCAACCGCTTGACTAAAACTAAAAACTTTTGCTCCTAGAGTATTAACTCCCTTGTCAAACAAGATAATAGGTTTAACTCTTAGTAAAGTAGTAATGAACTTTTTCAAACCAGAGATTCTCCCACCTTTACGCATTTGCACTAGGTTTTTTAAAGTAACTGCAGAAAGGATATTTTGTTTATGTGATTCAACCTTTGCTTTAATTGTTTGATTATCACAACCCTTGTCAACCAATGCCTTTATATCTTCGACCAATCATTTTAGAGAAATAGCAATATCACTAGTTTCAAACACTAAAAATTCTTTGTCTTTATTTTGTTCACTAAGCTCTTTTGCTAATTGAACCAACATATCATAAGTACCACTTAAACCTTTACTTAAAGGCAGAAAAATAAAGCGATCATACTTAGTTTTAATCTCTTCAAAGATCTTAAGAAGATCACTTTGACGTGGTAAGGAAGTTGAGATGTTAAGTCCATGTGGGTTTTCTTTTAGAAGTTTATGAACATGATCATAATCAATTTCTATCCCATCACGAAAGCTTTTTTCACCATCAACAATTACTTGTAAAGGCAAGATGTAAACGCCGTTAATCTCCCCAGGTTTAATAGAAGCAGTTGAATCAGTGATGATAGCTGTTTTCTTCATGAATTAATCTCCTTAGTACCTTTGTTTCTTTAAATTATCTAGAAACTCTAGCACATAATCAAAAAAAAGTTTAGGCGCTGAGTCGTGTGGTGAATGACCAACACCATCAATAACCTTAAAAATAATCTTGTCACTTTTATTAGCTAGATAGTCTACAGAAGCTTTGGTTGGCGTGACAATATCATTAGCTCCTAAAATAACTAAGGTAGGTTTATTACCAATCATTTCATAAGCCCTTTCAAGTGAGTCATTACCATATTTAGCGTTTTGAACCATATCACTGTAAAGTGTTTTAAAGGTAGTTCTTTTTTTAAATGCATTGATAGCTATTTTCAAAAGTGATTTACGTTTTTCTTCATGTTCAACAAAATCCTTATGGTTGCTGTTATTACGCTTAAAAAAAGTATCTAAAATCCGCTTTTTATTAACTGAGAAAGAAGTTTGATTCATAGGAGCTACTAATATTAAAGCCTTAATTTTTAGTGGTATAACTTTGTTAACTAAAACAGCCACAGCACCTCCCATACTATGACCTATTAAAATGACGTTATTTAGTTTTTTTTGAACAATAAAATCACAAACCAAATCAACAAAGTGGTTTAGTTTTAATTGATCTGTATCTGTTGATTCATTGTCTCCATGACCGGGAAAATTAAAAGTAAAGAAAGGCCACTTTTTCTTTTTAAACAAGCTAAAAATACGACTAAAACTGGCATATTCGCTTCCAAATCCATGTAAAAAAATAAAAACGTTTTTTCTTTTTTTAGGTTTAAAGGCAAATATTGAGTTAAAAAGAGTATTTTTATTACTCGTTAGCATTGATTTTAGCTAAACGTTCTGCATATGCTTGGATGCGTTCTTTCTTAGAAAGTTTAGCGAGTTTATCATCCTTATTTTGGATAACAAAATCACGTTTTTTAATTCGTGGTTTTTTTATTTCAACTGGTTTATCTTCATCACTAGCTTCTTTGATCTCTTCAGTTTGATTAGCACTAATGTCATTGAAATATTGCAACTGATTTTGCAGTGCTTGGTTTTCTAAAAAAAGTTGTTCTTTTTCAATTTTTAATGTTTCTAGTTCATCAAGATGTTCATCACTGGTAACTGGAGTAAATTGAAGTTCTTTTTTAGCATTTTCAAGTGAATTTATTTGTTGTTTTAACTGATTATTTTCACTATCAGATGCACTTAAACTTTGCTTGGCTTTCTCCAATTCACTGTTTGTTTCTTCAAAAGCAGAGAGTAAATCTTGGTATTGTTCATTAGCATCATTCAATTTATTCTTTTTAAGCTTTTCAAAATCAGTTTTTAATTTGCCATAGCTTTCTTGTAAGATTTCATGATCAGCTTGGAGCTTTGCTAATAAGTTATTGCTATTCTCAGAGCCTGATAATTCATCTTCTAAATCATTAATTTTTTCATTAAGTGCTAAATTAACTTCTTCAAGCGTGGCTAATTTATCTTGAGCAACATTAAAAAGATTTTGAGAGTCTTTGAGTTGCTGTTGGAGATTTACTAACTGATTGGTATTTAAATTACTCTTCTCTTCATTCCATTCTTCTAACTCTGCAATTTTGTCTTGCAGTTGTTGATTTTGAATTGTTAAACCATTAAGTTTTTCATCGAGTGCTTTTTTCTGTTCTTCAAATGAATTTTTAGTGTTATTGAAGTCATTATCTTTAGACCTTGTTCACTCAATTTCATCTAATAAAACATCATTTTCATGTTTAAGTGCTGAAATTGTTTTTTGATAATCTAAGTTTAGCGCTTGTTGTTTCACTTGCAATTTATCAAATTTTGTTTCATTCTCATCAAAAAGTAGTTCATACTGATTTAAAAGATCATCATATTTTTCATTCTCATCATTAGATGTGTTGTCATGGAGTTTTTTCAGTTCATTTTTCAAAAAATCATTTTCTTGTTCAACTAGATCCAATCTTTGGTTTGCTTCTCTTGCTGTTAGTAACTGGTTTTGAAGTTCGTTTATTTGGTTGTTTAAAACTGGTAATAAAGCTAATTGTTGTTGAACATTACTGCTCTTATTTTTCAATGCATCTAATTGATCTGAAAGCAAAGCTTTTTCTTGTTTTAAATTCTCCAACTCTTCATTAACTTCATCACTTAAAGGATTAATAGCAACTGGTTTATTTTGCAGTTGCTCATATAAATCCCTATTTTCTTGCAAAAGATCATCCTGTAATTTCTTGGTATGTAAGTGCTTACTTTTTTCCTCAGATAACCTCCTTTTAAGATCATCGATTTCCTGTTCTAAATCAAATATTTGATCATTATTATTCTCTTTAAGATCATCGATTTGTTTTTTTAAATCAGCATTTTCCAATAAAAGCTCTTTGTTATTATAGTCATCCAATTCATCAACTTTTTTTGTTGGATGTTGCTCTTTTAAGATCCTTTTAACAACAAAAGGTTGATCATCATTCTGATCATATTGGATCGTTTTATGAACTGGATTGATAGCTTTGGTTAAATTTTCAATGGAATAGAAATCATCAAATTGTTCAGGTTCTTTAACCTTATCAGTTGAAAATATTTGAACTTCTGGTTTTTTTGTTTCATTAATCTCTTCACTAGGTTGGTTAAACAACTCTTCAAAAGATACTTGTTTAGTAGCTTCTGGTTTTATCTCATCCTTATCATCTTCAGCAGTAATCACTGATGGTTTTAGTTCCTGAAAACCAGTTTTAATTTCCTTGGTTAAAACTGATTGATCATCAACTTCTTGAAAAGCCATAGTTTGATCAACTGTCTGTTGCTCTTCCTCTCTAGTTTTAAGTTGATTTGCATGATGATTTAAAACATTCTTATCCCATTTTTTATCTAAAACCTTTAAATCTATCATCCAATTAAAAAAAGAAACTATTGACTTTTCTTTCTCAGCTTCATCAACAGTACCAAAAAATGTTTTCTCCAATAAATTTCTTTCAAAGGGAATAGCAGTAATTTTAGATGGTGAAAAAAGGATATTATTAGTTCTTTGTACTAATTCTTTTAAAAGTGTTTTTTCATCAAGCTCATTATCAAAATCACTAACTTCAAGTTCCTTATCTACTGCCATGGATTATGTTGGAATATTAGATAAATAAGTATATTATCTTAAACTTCTGGCTTTGTTCTTATCCGTGCGTTTTTTGATTTAAAGTAAAGGCTAATAGGTACAGAATTAAAACCAAAGTTTTCTCTAATCTTATTCTCTAAAAAACGTGCATAAGAAAAATGAAGATACTTAGGGTCATTGCAAAATAAAACAAAATGGGGAATTTGACTTTTGGTTTGTACTGCGTAAGTTATCTGCAAACGCTTGCCTTTAAAAAGTGGAGGTTGATTGTAAAGTTGTGCTTGCTGAATAACATCATTTAAAAGTGGTGTTGCAACCTTAGTTTCAAGTTGGCTTTGAATAATTTTAAGTTGTTCAAAAATAGTATTTAAGCGCTGATTTTTCAAAACACTAATAAACAAAACAGGCGCAAAATCGAGGTGTTTAAAATGCAATTTCAGCATTTTTTTATATGCATTTGTGGTGTTATTGTTTTTTAAAACTAGATCCCATTTATTCACAAGAATAATAACAGGGATAAGTGCAGCTTGTGCCAATCCACCAATCACTTCATCTTGTTCACTAATAGGTTTTGATCCATCTACCATCAAAAGGATAACGTTACTACGGGCAATAGCTAGCTTTGTTTTGATGTAAGATGCGGTTTCAATTCCCATGTTAATTTTGCCTTTTCTTTTAATACCAGCAGTATCAATCAAAAGAAATTTTTCACCATTAACTTTTAAAGGAACATCAATAGCATCTCTAGTTGTACCACTCTCATTTGAAACTAAAACACGATTTTGTTTTACAAGTTGATTAATAAGTGAGCTTTTACCAACATTTGGTTTGCCAATTACACAAAACCTTATTTTTACTAAATCATCATTATTTTCATTAGGGAGTAATTGATTTTGTTTAACTAAGAGATCCATTAAATCACCAATTCCAATTCCATGAGCAGCACTAATAACAACTGGTCTTCCAAACCCTAAGCTGTAATAATCTTTTAATGTTTCTTCAGCAGTTTTAGGGTTAAAATTTTCAGCTTTATTTACCACTAGTATTACTGGTTTATCCTTATTTTTTTTAAGAACCTTAGCTACATAAAAATCATCACTATTTAGTTGTTCTTGTAGTGACACTAAAAAAATAATAGCTTTGGCTTGACTAAGTGCTGCTTGTGCTTGTAATGCAATTAGTTGTTGTAATGGAGTTTGTTTTGCAATTAATCCACCTGTGTCAATAAAAGCTATCTTTCTTTTTAACCATTCACCAATTCCAAAGATCCTATCTCTTGTTGTGTTAGGAGTATCTGAAACAATAGCCATTGGCTTTTGAATTAAGCGATTAAATAAAGTTGATTTACCAACGTTTGTACGACCTATTATTGCAACAGTAAACACAAATTAAAGTTTAAAAACCTTCTTTGCTTCTTTGAGGGTTTGTTTTACTACTGCATCAAAACTTAGTTCAGAAGTGTCAAGATAAATAGCGTCCTGGGCTTTTTTTAATGGGTCTGCAGTTCTAGAACTATCAATTTGATCACGTTGCTTTAATTCTTGAATTAGTTCCTTTAGTTTTTTTTCATTTGATAGAGAAATTCCCATATCTTGTAATCTTCGCTGCGCTCTAATTTCAACTTTAGCATCTAAAAAAAACTTCAATTGAGCATTTTTTAAAACTACTGTTCCTATGTCTCTACCATCCATCACTATGTTTTTATTTTCTGCTAGTTTCTGTTGTTTAATAACTGCAATTTTTCTAATGTTAGGATCAACAGCTATTTTACTAGCAATGTTAGCAACTGATTGGGTTGTAATAACTGTTGTAATATCAGCATTGTTATAATACACAGCATCTTTCTCAAAGCGTCAGTTAATTTGATTAATGATTTTTAAAAAAAGATCAATATTTAATCTATTCACTTGCATTACATAGGCAAAGGCACGATACATTTTTCCACTAGAAAAATAAAAAAAATCAAGTTCTTCAGCTATTTTTTTAGCAACACTGGACTTTCCAGAACTACTTGGACCATCAATTGCTATTTGTCAATTCATAACTAAATTTAAACAAATGAAACAAAACTAACAACCAAAAAAATAAGAAAAAACAAAATGCTAAACAGCATGAAACTTATTAAAGTTATCACTAAGTTCTTAAAAATAGCCTTATTAGCATTAAACAAATTAGGTTCGATGTTATCAATGCTTTTTTTAAAAGCAAAACTTTCAGTTGAAAAAACTGTTAGTATCTTGTTGTATTCTTTTAAATCATTAGTTAAATTGCTTGTTTTTTTTAATCATTGTTTCTCATTAGCTTCATAAAATTTACTTAAGTGGTTAATCTTTTCTATTCCCTTATCATCACTAATTCAGCTTTCATAACCGCTAGAAGTTTGGTTATATGCTGCGTTTAACCACATCCTATCAACTTTTTCTTCTAATAATTTAAAGTCAGTATTTTTTAAATCAACTATTAATTCCTGAAGTGTTTTAAACAGTTTATTTGGTTCAGTGTGATGTTTAACTGGATTTTTATTAAATTCACTAAAAGGATGGTAATTAATCAAGATTTTCTGATTAATTTTATTAAGCTGTTTTTTTCAGCTTTCAGTGTTCTTCTGAGCGTTAATTAAAGCTTTTTTAACTATCTTATTGTTATCAAAACTTTGTCTATAAAAGCGAAATTTTTCTACTCTTCCCATGGGTTGGCATTATGAACAACAAACTGAATGTCATCATCTTCCTCACAAGCATTGAGAAAACGTGATAATAAAGTTTGCTGATTTCTATCTAAATCAACAGTTAAAAGAGGTACCAATTTAATTTCACTATCTACTATTGAAAAATTGTTTTTTTTCAACAGTTCCTTTGCATGAAAATAACTATTTGAATGCAAGCTAATTTCAAAACACTCTTCATCATAATCAACATCAATTAAGTTAATATTGTCCAAAATTAATAATTCAATTAGATCATCTTTCAAATAGTTATTTTTATTAACAAAGATAATCCCTTCTTCTTGAAAATTAATCTTGACGCTATTTGGCTTGGCTAATTGTCCTTTTAGTTTAGCTAAATAACCGTTTAAACTACTGAGTAAACGGTTAGGATTATCAGTTAGTCCAAACACAATAATTCCAACACCATTTGGTCCAAAAATCTCATAACAAAACTCGCTAATTTTAGTTGTATCTTTTTCACTACCATGGATATTTCTTTTAATTGAATCCATGCTTAGACCCTTAGCTAAAGCAAGATCAACTGCAACTTTTAGATGTGGATTTGACTGGATATTAGTTCCACCTTTTTTAACAGCTGAAGCTATTCTTTTTGCTAGTTTTTGAAGTTGTTTTGCACTTGTTTGTTGTTTTTTATTAGTTTGATTAGCAATTAGATGCTTACGTGGCATGTAAGATTAAAAATTATTAGCAACTTGGTCAACTTTGTGTGCAAACAGATCAACTACCTCTTGCTTGTTTTTATCTTTGTAAAGAATTTTAGTGTCATCAATTAAAACACTCTCAAAATGTGTAACACCTGCAAAACCAAACAAGCCCTTTAAATTACTAACATGATCAGCTCAAGGGTATCATCCTAATGGTGCACCTTGTGACGCTAAAACAACTACCTTAAGATTAGTAAGTAATCCCTTAGAAGCACCTTTAGTAACATATTTGTCTTGAAAGGTTTTATTAGCTACAAAGACATGATCAATAAAGTTTTTTAAACTAGCAGGATAATTAAAGTTGGTCATTGGTGCAAGGATAACAATTCCATAAGCTGTTTTTAGAGCATCAATATAATAATCACTATTTTCAAAACTAAAAAAATTACTAGCGTTTTGGGTATTATAAGATATCTTACCTACTGGTAATTCATTTAAATTTCAATCAATAAATTCAACACTACTATTTTGTTTTTTATAAGTTTGAAGGAAACGATCTAATAAAAGGTGGGTGTAAGAACCAGAAGGGGTTACAGAAGCATCAACAATAATAACTTTCTTCATAGCTAATAATTAAAAGTCTAATTTTTATTGTTAATTCTAATGAGCTTTAGTTAATTTAGCAATTAATTGGTTGGTTTCAGTAAGTTTTTTTTGATATTCTTCCAATTTTAAAAATTCACTTTTTACCTTTTCTTTGGGTGCTTTTTCTAGAAAGCTTTTATTTTTAACAATAGCTTGGCTACGTTTTACTTCACTTTCAAAAAAAGCTTGTTGCTTCTGTAGAGATTTAAGTCTTTGGGTTGTATCATCAACAATTTGGTATTTAAAACTAACTTTTTTATTAGTTTCAATTTTCAGTTCAATTCAACTAAAGTTAAAGTATTGTTTAACATCAACAGCATTTTTACCAGATAAGATAACAACTAGTTTTTGTTGTGAATTAAGCATGTACTGTTTTCTGTAATTGCGTAAGTCATTAATAGCAGCTAAAACAAGATCAAAAAGTTTGGGAATTTTAATTTTAGTTGCAAGAGGTCATGTTGCTTGCATAACACTTTTATTGTTAAATTGCTGATAAATACGCTCAGATAAAAAAGGAACAGTGATACTAAGCAAAATAGCAATATTAGATAAAACTGATTTAGCAGTATAAAAAAGCTGTGGTTTTAGTTGATTTGGTTCTTTTTTAATTGCTTCAATGAAAGTATTGCAAAAATCATCCCAAACAAATTTAACAAGAATTTGGTTTGCTAATGCTAACTGGAATTTATCTAGTAGCTTAGTTATTTTTTGAATTACTTTATCTAATTTAGCTAAGATTCAAGTTTCACTTAATGAAAGTTTGTCCAAGTCATAACTAATTTCTTGATCATTTTCTAGTTGGATAACAAACTTAGTAACATTCCACAATTTATTTAAAAAATTTCATGCACTTTTTATTTTTTGTTCGCTGAAAATTAGATCATCTCCTGGAGTGTGATTTGAACACAAAAATAAGCGCACTGCATCCGCTCCATAATTTCTAATAAGATCAACAGGATCAATGCCATTATTCAGTGATTTTGACATCTTACGATTTTGTTCATCGCGTACTAAACCGTGGATTAAAACAGTTTTAAATGGCAGTTTTTTAGTTTCAAAAAAGGAGTTAAATAACATTCTTAAAACTCAGAAAAATAGAATATCATAACCTGTAACTAAAAGCTCAGTTTCATGAAAAGAGTCATCCTGTTCTCAATTCAAACAAATTAAAGGTCAAAGCGAAGAAGAAAATCAAGTATCAAGTACATCTTTTGATCTAGTGTAGTTTTGTAAATTTTTTGAAGGTTTTTCACCAACAACTATTTCACCTGTATTGTTTTCAAATCAAACAGGAATTTTATGACCCCAAATTAACTGTCTTGAAATACACCATGGTTTGAGTTTATTCAACCAATTTGACACTTGCTTATTAAAGCGTTTGGGAATAAAATCAGGATATTTTTTTAAATATAAGTGATCTTTTAACTTTGGTAAATCAACAAATCACTGTTTTGAAAGCATGGGTTCTACAACAGTGCCACTGCGTTCAGAAAAACCAACATTACTAGTTAATGGTATTGATTTAACAAGTAATTTATTTTTTTCTAATCATTTAACAATTTTATTTCTTGCTTGTAAAACACTAAGGCCTTGAAATTTACTTGCATTTTGATTGAGAATACCGTTACTGTCAATGCAGCTTAGAAAATCAAATTTATATTTAGTGTTGATTTCATAGTCATTAAAGTCGTGTGCAGGAGTACATTTCAATATTCCTGTACCAAATTTAATGTCAACATAGCTATCTGTTACAACAAGAATTTGTTTTCCTGTTAAAGGGTTAACTACTAATTTATTTCAGAAATTAGTATAGCGCTTATCTTTTGGGTTTACCAATAGACAAACATCAGCAAAGATAGTTTCTGGTCTTGTTGTTGCAACTATTAGTTCTTGTTTACTATCATTCGCTAGTTTATAAACAACATAATGAAGATGTTGATTAACAGGTTTATTGATAACTTCAATATTTGATATAGCAGTATTCAATTTTGTATCTCAATTAACAAGCGTGTATGCTTGATAAATAAAACCATTTTCGTAAAGGTTTTTAAAACAATTGTTAACAATTTTATTAGCTTGTTCTGAAAGTGTGAATTTAGTTTCAGATCAATTTAAGCAAACTCCTAAACTCTTTAGTTGATTTTTAATTATTTCGCTTTGATTTAATGCCCAATTCATGATCATTTCAGATTTTTTATCATCATCTGCATCAAAATATTTTTGATTTTCTTTTAATGCTATTTTTTCATATTTCGTTTGAGTAGCAATGCCAGCATGATCAAAGCCAGGAATTCAGTTAATACTAAATCCCTGCATCTTTTTAAAACGCATGATTTGATCAGTAATACTAACCTCAAAAGCATGACCAATATGAAGAGTACCTGTTAAATTTGGAGGGGGAAGAATTGCTGTAAAAGAATTGTTTTTATCTTTAGGTTTAAAAAAACCAGCATTATTTCAAATTTCATAAAGCCCATCACTAACTAAGTTGAAATCATAGTTTTTTTGAAAACTAAATTTATCTTTCATCTTCAACGAATAATTCGAAAATTTTTTTGTGAACTAAAGCAAGATTATGATGTGTTTTTGTACTTGCAAAAACACATTTATCAAAATTAACTTTTAAAAAATTTGCAATATTCTTAAGCGAAAAATATCTTTCACTTTGATTTAGTTTGTCAAATTTATTAGCAACAATCAAAAAGTTCAATCCTGTTTGCAAAATAATTTTAACCACTTCCTGATCTTGAACAGTAACAACTCCACTATCTACTATCAAAACAACACCAACTAGATTACTACGAAAATTTAAAAATTGGGTAAGTAAATTAGTAATAAAATCTTTCTTATTTTTGCTTATTTTAGCAAAGCCATAACCTGGTAAATCAACAAATCTTTTGTCTTTATATTCAAAGTAATTTAGTAATTGTGTTCGACCTGGTGTTGCTGAAGTTTTAGCCAGTTTCTTTTTAAAAAAAGCATTAATCAAACTTGATTTACCAACATTACTTCTTCCCATGAAACAAATCTCTGGGATATTATCTTGAGGACAATCTTTTAAATCACTTGCACTCTTCAAAAAATGTGCATCCATGTGTATTTTATTTTTGGTTTTTAATGGAGCGCATTATCTCTTTTACCTGGGTTTCAGATAATTTTCTACCCATTTTTGCATACATTGCTTTAATTTGATTTTCAGTAATAGGTGGATTGTCACGCATCTGTTTTTTAAAGATTTTTATAGAAATAAAATAACCAAGAATCATTCCCACCAAAAGCGAAAGAGGGATACCTAAACCTAATGCAAGTGCTAGATCATTCATAGCTATATATTAATAATTAAACATTTTAAAAGTCTTATAAAATTAAATAATCGAATTAATGGATAAACTTGTTAGTATATTAGTTCCTTGTTACAAATCAAAACCTTTTTTAAAACGTTTTTTTAATTCACTTTTAAAGCAAGATCTTAATCAAGCTAAAATTATTTTTTTCAATGACAATGTTGCTGATGAAACCTATGAAGTTTTGCAAAAATTCAAAAAAGAACACAATAACTTAGCAATTGAAGTCTATTGTGACAAACAGAATGAAGGTATTGGAAAAGTGCGTGATAAGCTAGTGAATCTAGTAACAACACCTTATTTTTATTTTATCGATCCTGATGATTGTTTTAACAACAAAAATGTCATAAAAGAGATTGTTGAATCAATTAAAAAAGAAGATTTTGATCTTGGTGTATTAAAAAGTATGGTCTATTTATGCTTCTTAAAACATGATTTCATTATTAAATTTTTGCCTTTAAAAGGTATTTTTCAAGGCAGAGTAAAATTAATTAATAATAACAATGTTAATAAATTAAATTACATCAAAAATAATGATCAATATATTTGAAATATTGTTATAAACACAGATTTTTTTAGGAAGCTAAATTTAACTTTTGAATCAAGGTTATTTGAAGATATACCAATCTGATATCCGATGTTTTTTTCATCACAAAAAATTGTTTTTATTGATGTGATAGGAACAAATTATTTTATTCGTAATGATAGTTTATCAACTACTATTAGTGCTCCACGCTACTTAAATTTAATCCAATGTTATGAAAAGCTATATGTAAATCTCAGCCAAAATGGTTCTCTTGCAAGTTTTATTGATCCAAATCATAAGATTGAAGCTAGGTTTTGAAGAAGGCAAATGTTTGTTTGATTTGCACTTTTCAGCTTTGAATACTTTAAGAAAAATTTTTCTGAATCAAAAAAAATTCTTGAAAAACTATTTGTTTTTTTGGAAAAAAATGGAGTTTATGAACGTGTTTTTCAAACAAAAAATCAAGGTATTTACCATATTTGGGTACAGCGACTAAAATATTTTAAACATGTTTTGGAATCTAAATCAGATAACTAATTAAGTTCTCTTTAAAAAATCAATGAGATTAAAGTTATTTAAAGCTTATTGTTTAATTTACTTAAATTAAGAATCTTTATCTTGTTAAATTAATACTTAACTGGTTTAATGTCTGCAATTAAATTTAATCCTAGTTCATTCAGAAAAAACTTTAAATGGTTTGAAAATAACAAAAATTGGATTAATTTTGATAATGCTGCTACTTCCATTGCACTTGATGTTGTGGCTGAAGCAAGCAAAGAATATTACCAGTATTTTTGTGTCAATCCTCATAACAAAAATCCTGAAATTAACCAAAAACTTATTGCTATTATTGAAGAAACAAGAGATTTATTAGCAAAATTTTTCAATGCTAAAAAAAATGAAATAATTTTTACAAGTTCTGCAACTGAATCGCTTAACTTATTCGCCTTTGGATTAAGCTCTTTAGTAAAAAGTAATGATGAAATCATTCTCAAAGAAGATGAACATGCTGCTAATGTTTTTCCCTGAGTAAATCTAGCAAAAGAAAATAAAGCCAAACTAAAAATAATTAAAAAAACACCAAATAAATCTTGAACTGATGCTTTTTTAAAAGCTTGTACACCATCAACAAAACTATTAGTTATAACTGCAACATCTAATCTTTTTGGAAATAGTATTGACTATGAAAAAATTTCTAAACACTTAAAAAAAATATCACCAAATAGCTTTATTGTTGTAGATGCAGTACAAGCTGTACCACACCATAAAATCGATATTACAAGTGCTAATATTGATTTTTTAACTTTTTCTACACATAAATTTTATGGACCTACTGGTCTTGGCATTGCCTTTATCAAAAGCGAATTACAATCACGACTAAAACCCTTTAAATTAGGTGGTGATATTTTTAAATCATTGGATAATAACTTTAAGATAATTTTTAAAGAAGGTCCTTCCAAATTTGAAGCTGGAACGCTAAATATTATGGCTATTTATGCTTTGAATAAACAGTTAAAATTCATGCAAAAAGAATTTAATTTCAGTGAAATGGTGTTTTACAGCAAACAATTAAAAAATTTAGCTTATCAACTGCTAAGTCAAAATCCTAATATCGTTTTAGCTAATCATGATCAAGATGTTCCTATCTTTGCTTTTAAGCATAAATATATTAATTCTGCAGATCTAGCAACTTTTTTAAACATTAAAAAAATAATTGTTAGACAAGGATCCATCTGTGTTGGTAAATTTAAAAATAAAGAGAGTTTTTTACGTGTTTCTCTACTCCATTACAACACAAAAGAGGAATTACTTTATTTAGAAAAATTATTAAAAACTAGTAAGAATTCCATTATTAATGAACTAATATATTAGCTAATGGATATTAGAGCAAGAACAAAAATTATTGATATTTATTCAAATTTAAAATACAAAAAACCTCTCAAAAGTTTTCAAAAAATTTTAACAACAAGTGATAGTGATAATTGTGAAGACTTTTTTAATATAGGTTTAAATATTGATAAAAATAAAATTACTGCAATTGGTTTTGATGGTGATGGTTGTATTATCTCTACAATTGCAACTGAATTAAGTATTAAAGCAATTGAAAATAAAACTATTAACCAAGCAAAAAAAATATTATCAGATTTAATTGCAACTTATAAAGATAAAAATTCAGCAAACCAAGTTGTTGAAGAATTAAAGCTTTTAATTGAAATGAATGTAACTGAAAAAAGGTTACAGTGTTTGCTTTTAACACCAAGCAACTTATTGCAATGGTTTAAAAATTTTTAGCATTAATAGCTAACGTTTGTCCTAGGATATCATATAAGCGTTTATCACCAACTAAAATCTGTTGATGTTCTCTTTGGTGTTGCTTTTGATTTTCACGAATTAAATCTAAAAATGCCAACTCTTGAGTGCCTTCATCTAAAGCTTGTTCAATAAGTAAAGACATAAAGATCTCTTTCTTTAAACCTAATGAATTAGCTTTATTATTTTTTCAATTACTAGAAATCTTATTTACATCATCATTTGTTATCTGAATAACATAACTAAGAGTCTGGATACTAGAGTCATTTGTTAATGGAACTGTTTGCACAGTTTTATATCCTTCTGTATTGGAACCATCTGTATTTGCAGCTTTAGATAAACCAACATAACCTTCAAATTTATGAAAATAAAGATCATCTAGTCTATCAACTACATTTAATAAAAACTTTTTTTTATTATCAAAACTTATTAAAGGTTCACCTCTAGAGTTAAAGCGACTTAAATTATTTGTATCAACATTAACTGAGCGATGTAATAACTCACCAAAAGCATTAAGTTCAGCATCAGAACTGGTTTTATTAATCAAGCTTCACAAGGTAAACTTATCCTTATATTGACTTAAAGCGCCTGCTTTATCAGTTGCTGTAATTAACTGTCCGTAATTATTAAAAAGCTGTTGTGTTAAACCAGCACTAGCTGTTGAAATACTTTGATTGGAATTAATTGTTAAACCATTAAATCCATAAAAATATTTTTTATCACTTGATCCTTGTGATTTATTAGATAAAGCTACAGAATTATTAAACGGTTCATCAGTAAATCTATAAGTGTTTTCACTTTCAAGCAATGAATCGGGTTGAATTGGTGTTTGTTTATCATTGAATCAGTTTATTTCAGAACCTTGAGTATAGTTAGGATTTTGTAAAAACCTATTAAAAGGGTTTTCATTAGCTGATGAAGTACTGGATTTTGTTTTTTGACTAACAGTAGCGCTAGGGTTTTTATCATATCCTACTGTTCATGAAACAAATGCTCTAGTGCCAATTGGAATTGCTTGTTGTAAAAGATTCTTATAGTTTTGAGCACCATTTTTAACAAGTCACATTAACGCTTGCAAAAAGCGCATGTATTTGTACTCAGCATCCTGATTAGGATCTTCCAATTCCTTTTCAAATGCTTTCTTTGCTAAATCTAATAGGGTTTGATTAACACTACTAGAAGTATGAGATTGGGCAGCTTTATTTTGCATTTGCAACATAGTTGAAGTTGAATTTACACTACCCAAATCCTTATAGTAACCAAAGCGTTCTTTACTAGTTAAATTTTGTCAAGAACGGATATAAAGTGCCTGTTTAACAAGATCATTAAAATCAGAAAATTCACCTTTTAATTCTTTATTTGAAGTATCAAAATTATTAGTGAATGAATTAGTGTTTTTAAAGTACCAATTTTTAAAGGTGTTTGCAACAGTACCTGTTCTAATTCCTGAATCAGTTAAAAGTGATAATATAAGATCAAGATCCAAGTCATTTAATTGTGATGATTTTAGGGTAATAATCTCTGAATATTGACTAGAAGAATAAGTTGTAGCTTTTCATTTTTCAACTAATTCTTTGGCTTTATTTTCTACTTCAGTTGTTAAAGTCTTTAAAGTTTGATTATTTTTTTCACGAGTTGGTTCATTATCAGAAGTAATAGCTGCAGCAGTGGTTTTCAAAGTTAAAGTTTGAGCACTACTTTGATCAGAGTTGTTATTAAAAAATTTTGCTAATGAAGGATAACTACCATCATTTGCTCTTTTATATGGAAGAGGTTGACCCCAACCAATTGAACTTTCTTTAGCTTCCTTTTGAAGTTTAATGTAGTTGTTAGCACGATCAACAATTGCTTGTTCTAGTTTTTCTATATTAGCTAAATCTGTTCTAAAGTTATTAAGTTTATTAGCAGCTTTAATATAAGACTCATAACTTGATTTTATTTGTGAAAGTGAATTATTAAAATCTTGTTGTTTTTTAATAGTTTCAGATAAATTGGTATTGTTTTTTAAAAGTTTTTTAGCTAAATCACATACATCTTTTTGACCGTTATTATTAGAAGTTCCATTTTCTAACATAGTTGTTAAAGCTGTGTAAAGTTTTAGTGTTGTATTTCTATTTGCTCAACTTCTAAATTCGCTGTTAGAACCAAATAAATCAACTGAAAAACTGACATTATCTTTAGAGAAATTATTAGTTTGTCCTTGTAAAAATCGCATTAATAAAACTTCTGCTTGTTTTTCAAAATTACGTTTGTTAGGTGATGAATTCTCTAAAAAATAATTACCTCCATCAATATGAAATGCATATATGGCATCATCACCTCTAACATAAACAGGTTTCTGTTGCTGTTTTTGAGTTGTCTTTGAACTTAGACTAACTAATAAATTACTTGTTACTACTTGTGCTTGAGATGAATTTGATTCCATTCTAACTGCATCAAAAATAGTTGCAGTTTGACTACTATTATTTAAAGCTTGTACTTGTTTTCAAAAAGACTGCGTGTTACTACTATTATCACTACCAGAAGAAGAAAGCGAGGGATTAATTTTTAAAAATTCACTTTTAACATTAAAACCATTATTGTCTGAAGTAACTTCTTGACTTTTTGTCATAGCACTCTTCATCATAACTGTTGTTGCACTTGAACTTTTAATAAATTTATCAAGAGGATTTAAATTGCTACTAGTTCCACCGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTAATTCTGTTTTATTATTCAGTAAGTAATGGTATTGATCAATAACTGCAGCAGATAAGTTAACAGTTTTTAAAACAGAGAGAATGTTATTTTTACTAATTAAAACTTTGCTTGCACTATACTTGTTTTCAAAGTCAAGTAAATCATTTCTGGTATTAGTAGCAGCTGAACTTTGTGATGAACCACTAGAATTAGCAGTTTGTAATTGTTTATCAAACTCTTCAAATAATTTTGCAGAGTTTGTTTGACCACCACTTTCACTAGGATCTTGAAAAGCAGGAAAGTTATAAGAAAGATTTAGATTGGAAGCTCCAATTGCATTTTCATCATAAATACTGCCAAGTCCAGCAATTGGAGATTGGTAATTGAAAGAAGCTTGATTTAATAAAAATGGGTTTTCTTCTATTAATCACTGTGAAAATAAATAAGCTTGAAGATTAGTAAAAAAATCACCATTTACATCATTTAAATTGCTGTTAATAAACTTAAGTTTATTTTCTGTTTTTTCAATGTTTTCTTTAGTTGTTGCAACGCTTGTATCACTTGAATTCACTAAACTAACATTTGCACTAATGTTATTGAATATTAAAGATTGAAAATCAGTAATTAATCTATCATAGAGTTTAAGTTTTTTTCAACTTTCTTGATAACCACCGAACTGATCATATTCTTTTTGTACTTCAATAGGTCACTTTACTCTATCAGCACGTTTCTTTTCATCTATCAAGTTATCATGTTCATCTTCAACATTTTCTTCAAATATCTTTAATCGATTAGAGATGTTTCTATCTGCATTTGCTTGGTATCATTTTTGTAATGCTGATCCAAAACTAACACGTAAGATAGAATCAACACCACTACCATCATTTAAAGCAGACTTTAAAACTACATCTGGGTTTATGATATTTTGATCACTAAAACCAATTAGTTTTTGAGATCGATTGAGAGTAGGAAAAAGATTAGCAGTGTTTATTGTGGAACAGGCTGTAATTATTGCTGTTGCACCCACTCCACTAAATGCTAAAGCAATTAATGATCAGCGCTTAAAACGATGAGCCATCTTATACTAGCAGTTTGATAATTATCACATTTAAAAATCTAGCTAATATAATTGCAAATCTAGATTATTAAAAAATGTATATTAATTATAAATAAGTTAATTTTTCTAATTTAAGCATTAAAAAAGCTTAAATCTCTCTAATTAAAATGCTTTACCAGAAAATCAAAAAATTAGTTTTTAGCTAGCTGTTTGTTGAAATGAATTTTTATTAGCCAATTGTTGAACAGTTAGTTCTTTTATTTGTTCAAATAATGCTGGCTTTTCTGATAAAACATTTAATAATTTTTCTTTACCAACAGCAATGCTTTCATTGTTAAAAGAATAAGAATTTCCAGCTCTTACAACAACATTAAATTTAAGTGCTAAGTCAATAACTTCATGTTCATGTACAAAACCACGGTTAAACATGATTTCTAATATAGCAACACCAAAAGGTTTAGCAATCTTATTTTTAGATACCATTACTTTTGTTTTTATGCCAACATAATTGTTGAATTTATCCTTAAGTAATTCAACACGCTTAGCTTCCATTCTTAATGAACTATAAAATCTTAGAGCTTTTCCTCCTGTTGTAACTTCGTTATTTCCAAACATCACTCCTGGTTTTTCGCGTAACTGATTAATGAATAAAACACAAGTTTTAGAATCTGGTAATATCGATTGTATTCTTCGCAAACCTTTTGACATCATTCTTGCATGCAAGCCAATAGTTTGTTCTTCAATGGTGCCTTCTAGCTCTTGTTTAGGAATTAACGCTGCTACAGAGTCAATAACAATTAAAGATATCTTGTTTGTTTTAATTAATGATTCGATAAGAGCAAAAGCGTTTTCACCATGCCTAGGATGAGCAATCAAAAGTTTATTTAGATCAATACCAATTGATTTAGCATATGCTAAATCAAGTGCACCTTCAGCATCAATATAACAGGCTGTTTTACCTGCTTTCTGAAAACTAGCGACTGCATTTAGTGCAATAGTTGTTTTTCCAGATGATTCATTTCCATATAGTTCTACTATCCTACCTAAAGGCAGACCACCAGACCCTAATGCTTCATCTAAATTTAAACTTCCAGTTGAAATTGTTTCAATTTCACTGTTTTTCTTTGCATCAAAAAAATCAAAACTTGTCAAATTATTACTTTCAATAAAACTACTATTTTTTTGAGTATTTTTCTTATTAATTATTTCTTTTTGAGCCATCTTTCATTACACCTTATAAATAAGTGCAAAAAAATAAAAATGACATTTTTTTATTAGAAATTTTGTTTATTTTTTAATTTTTCTTAAAAAAATCTCTTTTTAGGAGTTTATTTTTTTATATTAGTTAACTTGTTTGGTTTGTTGACATAAACACTCCTAAAACTCCTATATATTATTAGTAAATATATAGATTTTTGTTCAAGTCTATTCAGGTAAAAGTTAAGTATGACAGTTCAAGTGGCAGTAACAACCAGATTAGTTTTGATTCAACTAACAACAAACCCGCCTACATCGTTGAGTTTACTAACTCTACCAACATTGGCATCAAGTGAAGCGTGGTGAAAAAGTATCAGTTAGATGTACCGAATGTTTCTAGCAACATGAACCAAGTGTTGCAAGAATTGATCCTTGAACAACCTTTGACTAAATATACCTTAAACAGTAGTTTGGCCAAAGAGAAGGGCAAAAGCCAAGTGGCGGTGCATCTGGGTAGTGGTCAAGCAAATCAGTGACAATCGATGCGTAATCAACACAACTTAGACAACAATCCCAGTCCCAATGCATCAACCGGATTTAAATTAGACAAAGGCAACGCATATAGAAAATTAGATCAATCCTGACCAATTTACCAACCGATTGATGGGACCAAGCAGGGCAAAGGGAAGGATAGTAGTGGGTGGAGTTCAACTGAAGAAACAACGGCAGAAAGGGATGCGCCCCTTGTTTCAACAAGTGGAACATCAGATCAAAGTAATAAATTCACCAAGTACCTCAACACCAAGCAAGCATTGGTAAGCATCGGTATCTTGTTTGATGAAGAGGAAATGGCGAGGAATGTTATCACGCAACTCTATTATGCTTCTACCAGTAAGCTAGCAGTCACTAACAACCACATTGTCGTGATGGGTAACAGCTTTCTACCCAGCTTGTGGTACTGGGTGGTGGATCGGGGTGCAACAACTGATTCATCATCAAAACCCACCTGGTTTGCTAACACCAATTTAGATTGGGGAGAAGACAAACAAAAACAATTTGTAGAGAACCAGTTGGGGTATAAGGAAACTACCAGTACCAATTCCCACAACTTCCATTCCAAATCTTTCACCCAACCAGCATATCTGATCAGTGGCATTGACAGTGTCAATGATCAGTTGATCTTCAGTGGTTTTAAAGCGGGGAGTGTGGGGTATGATAGTAGTAGTAGTAGTAGTACTCAAACCAAAGACCAAGCACTTGCTTGATCAACAACAACTAGCTTAGATAGTAAAACGGGGTATAAGGATTTGGTGACCAACGACACGGGTTTAAATGGTCCAATCAATGGGAGTTTTTCAATCCAAGACACCTTCTCATTCGTTGTTCCTTATTCAGATAGTCATAGTAATCAAACTTCATCAGGACCCATTAAAACGGCTTATCCAGTGAAAAAAGATGAAGCTTCAAATGTAGCGATCAATTCTTTGATTAACGCTACGCCGTTGAATAGTTATGGAAGTGGAAGCAACTGATTCCTTTGATCCCACCCAAGGGTTGAAGAGTGATAGTCCAGTGAAGGATTCAGCAAGGGAAAGTCAAGAGAAACTCGAAACAACTGCTTCATCGATGAGTAGTGGTATGGCTACATCCACTCGCAAGGCCCTCAAAGTGGAGGTGGAAAAACAAAGTGAATCAACTGACGAACTTACAAACAACGACTTTGCTAAAAAGCCACTAAAGCATAAGAACAGTAGTGGGACAGATGTGAAGTTAGATTCACAGAAGGATTTTACTGAAGGAAAGGTTTGAAAACCGGTGTTGACTACGGATGAGATAGAAAAAAATAGGGGGATGGGGATGCCAACTCCCCCTCCCCTTCAACTTCTGCTTCTTCTTCTTCTTCGAACTGGGGCTGGGTATCGCGTTCAAAGTGCTAATCAGAAAAACGGCATCCCTTTTGATAATGTGAAGCCTAGTAGTAGTAGTAGGTGGCTCCTCCAAACCAACCACCTACACCCATTTACCAAACTCCATCAGTCCCACCAGTGACTGGATCAACGCATTAACCTTCACCAATAAGAACAATCCACAACGCAATCAGTTGTTGTTAAGAGCACTGTTAGGAACTATTCCGGTATTGATCAATAAGAGCGGAGGAAGTGGGAATGAGTTTAACCATACGAATGATCAGAAGTGGGATAAAACGAATGAAAAAGATGGGAATTTACCTGGGTTTGGGGAGGTGAATGGGTGAAAAATAGTAACTATTTTTTTAAATTAATACTCTTCAGCAACTGTTTTGCTACCTAAGAAAGTTAACTCTTCATCAGTTAAAAATCCTGTTCCTGCTGGAATTTTTCCACCAATAATAACATTCTCTTTTAAACCTAAAAGATAGTCTACTTGGTTCTTAACAGCAGCATCAGTTAGGATTTTTTTGGTATCTTGGAAGGATGCAGCACTTAAAAAGGAGTTAGATTTACTTGCCGCTTCATCAATTCCAAACACCTGATTAATTGCAATAACAGGCATCTTTCCAGCTAAAAGTAAGCTCTTATTTAGTTCATTGAGATAATGGCTATGCACTAACTGACCAACAAATAAATTGCTGTTACCTGCATCTGTTACTTGCAACAAATTAGTTAGTTGTCTGATAATAATTTCTACATACTTATCAGCAATATCTATCCCTTGAATCCTATACACTTTTTGGATCTCTACAATCATGTATTGTCTTACCCTTTGGATACCTGCAATTCGCAAAAGTTGTTTAATATCAACAGAACCTTCTGTAATCTTAGAACCTGGTGAAACTTGATCACCAACATGAACACGTATTTGAGCACTGAAAGGGATAGTATAAATCCTCTCATCAACGTTTGATTTAATCACTACTTCCTGAGCGTTTTGAACAGTAGTAATTGATTTAACTGTTCCTTTCACTTCAGAGATAACTGCTTTTTCAAAATCTTTAGGAGTAACTACTTCAAAGATCTGTTTTAAACGTTCAAAGCCTTGTGCTAAGTTGTTTTCAGTTGAAACTCCCCCAGTATGGAAAGTACGCATTGTCAATTGGGTCCCAGGTTCACCAATTGATTGAGCAGCAATCACCCCAACAGCAGTTCCCAATTCCACCAACTTACCAGTTGACAAGTCAATGCCAAAGCAGTATTGACAGATACCATTTTCCCTTTCACAATAGATAACAGATCTAACTAAAACTTCCTTAATAGATGTTGCACAAATCTGTTTAGCTAATTGCGTTGTAATAAGACTGTTAGTTTCTACAATAGTTTTTTGTGTTTCAGGATCAACTATAGGAGTAATTGAGTAGCGATTAACAATCCTGTCAAATAATGATTCAATCAAGGATTTGGTTTTGGTTTCAACAATTGCTTCAACAACAATTCCTTTTCTTGTTCCACAATCATCATGGTTAATAATTAATTCATGAGTAGCATCTACTAGCTTTCTTGTCATATAACCAGACTTTGCTGTTTTCATTGCAGTATCTGTCATCCCTTTTCTCGCTCCATAAGAAGAGTTGAAGTATTCATTAATGGTCAAACCTTCAAAAAAGGAGTGTTTAATAGGAACTTCTATCGTATCTTTAATGATCTTAGATTGGTTATTTCTTTCATAGTTAAAGCTTTTAGACATTAAGCCGCGCATCCCAAATAACTGGGTAAAGTTAGAAATGTTACCTCTAGCGCCTGAATCAGCCATTACCACAATGGAATTATCACGGTACTGTTCTTGTTTAATTAAGCTTTGGATCTCATCAGATACTTTTTCTTTCACATTGTTTCATAACTTCACAACGCGTTTATAACGTTCATCATCGGTTAACAAACCCTTGTTATAAAACTGTTTGTATTTCAGCACCTGTTGATCAGCACTATCAAAGTAATTTTGTTTATTAGTGTACTTAGGGATATCAAATACTGAAACAGTAGTTGAAGAAAAAGTAGAGTACTTAAAGCCAAGTGCTTTGATATTATCAAGTGTTTTAGGAAGATCTTTAAATTCCAATAAAAGATAGAGTAGATCAATTAACTTGGAAATAACCTTTTTGGAAAATGCAGGGATAATTTGATGCTTTAAAATTGCTTGTCTTACATCTTCTCCCTGTTTCACAATGAACTTTTCAGGACATGCATTTTCATCAAATTCCCCGTCATTAATATAAGGAACATTATTGCCTAAAACATCATTAAAGATAATCTTACCAACTGTTGTTATTAAAGTTCCTTGGCATGCAAACTTCTTATTGGGAAATGCTTTAGTACTTATCCCTACAATAGCATGTAAATGAACCTTTTGACTTTCATAAGCAGCACGTGCTTCATAAACTGTGCTGAAGATAATCCCCTCTCCCAACTGTTCTTTTCTCTCTGTTGTTAGATAATAGTTACCTAAAACCATGTCCTGAGTAGGAGTTACAATGGGTCTTCCATCTTTCAAACCTAAGATATGTTTTGATGCAAGCAGAACAGCTCTTGCTTCATTAACTGCATTATCACTTAAAGGAACATGAACTGCCATCTGATCACCATCAAAATCAGCATTAAATGCAGTAGTTACTAATGGGTGTAATCTAATTGCTTTACCATCAACAATTCTTGGTTCAAATGCTTGGATCCCTAACCTATGTAGGGTTGGAGCACGATTTAGTAAAACAGGACGATCTTTGATAACATCATAAACTATTCCCCAGATAAGATCATCCTGGTTTTTAATCATATCTTCAGCTTGTCTGATGGAAGCGGCAATTGGTCTAATCTCATTACCATTTTCATCAAACTTATTGATCAATCCATGGATAATAAAGGGTTTAAACAGCTTTAAGATCATTAGTGCTGGGATCCCAACTTCATACATCTTCAATTCAGGGCCAACCACAATTACACTTCTACCTGAATAATCAACTCTTTTACCAAGTAAGTTGTGTCTAAATAAACCTTGTTTTCCTTTTAAACGATCTGTTAAAGATTTCAATGACCGTTTGTCTTTGGAAAGTGATGGTTTATGACGTGAAGAGTTATCAAATAAAGCATCAACAGACTCTTGTAAAAGTCTTTTTTCATTGTTAACAACAATAGAAGGTACAGTACCATCTTCCAAAATCCTTCTTAATCGGTCATTTCTAATGATTACCCTGCGGTAAAAATTGTTGATGTCACTGGTGGTAAACTTAGCACCATCAAGTTGGATAATAGGTCTGATATCAGGGGGGATAACTGGAACAGTATGTAAGATCATGTTTTTAGGATGAAGCTTAGAATTTCTAAACCAACTAATAGTTTCCAGTTGTCTTAAGATCTTCTTAACTTTAGCATCTTCAACACTATCTTTCTTGGCTTTTCTTAAAGCATCATTTAACCTGCTAAATTCCAAGTTAAGATCGATTTTATTAAGCAATTCCAAAATTGCTTCAGCCCCTATTCCAACCCTAAAACCAGTGTACTTCTTAATGTAATTAAAAGCATCATTTAAAGAAAAGGGGAGAGAGCTGTTTTTCAAACTTTCATAAAAGATTTTTCCCTTACGGTAATCTTCACTACTCTTACTTTTAATGAGATTTCTGAAGATATAACCTATCACACGACGCATTTTTTGTCGTGTTGAAAGTGAACCCTTACCAGTTAAGTCCAAAACTTCTTTGAACTTAAAAGGCATGATTTTATCATCTTTGATCTTACCTGTATCAAGTACTATGTAGTTAACAAAGTACAAAACCTGTTCAACCTCTTTGTAAGAGATGTTTAAAACTAATGATATTTTGGAAGGAGATGGTAATTCTTTTGACATCCAAATGTGAGCTACAGGACTCACAAGTGCAATATGTCCCATCCTTTCTCTACGTACAATAGATTCAGTAACTCACACCCCACAGCGATCACACCTCACACCACGATATTTAATCTTTTTGAACTTGCCACAAGCACATTCATAGTCCTTAACAGGTCCAAAGATTGCTTCATCAAACAAGCCTCCAGGTTCTGGTTTTAATGATTTATAGTTAATGGTTTCAGCTTTTGTAACTTCCCCTTCAGATCAGTTCAAAATGGTGTCATTGGAAGCGATGGAAAGTTTAATTGCTTTAATGTTTTTATAAAGCTTGTTATTTCTTTTATTACGTCTTGTTGTTGTCATTGTTAATTTCTAATAACCCTCAGTGTCAAATTCAAAATCATTGAAAAATTCATCTTGTTCCCCATCACTTTGCAAGATGGAAACATTATTGGAGTCTTGTTGGGTGTTGTCATCATAGATAAATGAAACAGATAAAGCCAAGCCCTGTAATTCTTTTGTCAATAATTTAAATGATTCAGGGATACCAGGCTCTGGGAAAGCTGCACCTTTAACAATAGCAGCATAAGCCCTATTTCTTCCTTGTACATCATCAGATTTAATGGTTAAAAGTTCTTGCAAGTTGTAAGCAGCTCCATAAGCTTCTAATGCTCACACTTCCATCTCACCAAACCGCTGACCACCATTTTGCGATTTACCACCTAATGGTTGTTGGGTGATCTTAGAATAAGGGCCAACAGCACGAGCATGGATCTTATCATCAACCATGTGATTCAGCTTCATCATGTACATAATTCCAAGTGAAATAGGTCTTTCAAATGGCATTCCACTCCTACCATCAATGAGTTTAAACTTACCCTGATTTTTTTGGGGATCTAATCCTGCTTCTTGCATAACATCTTGGAGATCTAAGAAGTTCACTCCTTGGAAAATAGGGGTTGCAATTTTGTAAATAAGATCATCAAAAGACATCCCTATCTCTTTCAAAACTAAACTAATGTCACTGTTATCGAGTTTTTCAAGTGCTTCTTTTTCACTTTTAATATTGCGATCATTGATTTGGCTTTTTAAACCTTTAATTAATCTTTCAACCCTTGCTTGAGGTTGATTGATTTCAATGGCAAACTCCTTAGCTTTATTTTGATCAAAACAACTACTAATTAAAGAACGAACTGCTAGCTTGTGTGCTGCATAACCCAAGTGGGTTTCAAAAATTTGTCCTATGTTCATCCGACTAGGAACACCAAGGGGGTTGAGTAGAATATCAACTGGGGTTCCATCTTCTAAATGGGGCATATCTTCAATAGGCACCACTTTAGAAATAACTCCTTTATTTCCGTGTCTACCAGCTAATTTATCACCAATTTGAATCTTACGTTTTTGAACCACATAAACCTTGATCATTTCAATCACACCATCATTAAGTTCATCACCATTAGCAATTGAAAAACGTTTTACAGCTGAAACAATACCATCCCCACCATGGGGAACTTTAAGTGAAGAGTCTCTCACGTTTTGAACACTTTCAGGGAAGATGGCTTTAAATAGCTTTTCTTCAGGAGAGACTTCGACTTGACCTTTAGGGGAAACTTTACCAACCAAAACATCCCCTTCTTTAACTTCAGCACCCACCATAATGATGCCATTCTCATCAAGATAGCGTTTGTTTGCATCACTAACATTAGGGATATCACGGGTAATTTGTTCATCACCATTTTTAGTAGACAAACATTGAGCAACATACTCATTAATGGTTAATGAAGTGAGAATATCTTCCTTAACTAATCGTTCTGAAATGACAATTGCATCTTCATAGTTATAACCATTTCAAGTTGTAAAAGCAACTAAAACATTCTGTCCTAATGCCAACTCACTCTTATTAACAGCAGGGCCATCAACAATGATTTCATCCTTATTAACCCTTTGGCCTATTTCAACAATTGGTTTGTGGTTATAACAAGTATTTTGGTTGGAACGTTCAAATTTAACTAGGTTAACTGTCTCTTTTTTAGAACTATCACTTGTAATAATAATCTTACTGTTATCAACATAACTAACAACACCTGAGCAAGGAGAGGACATTGTTAAACCTGAATCACTAGCAATTTTGTGTTCTTGACCAGTACCTACTGCAGGAGCATATGGCTTTATTAAAGGATAGGCCTGACGTTGCATGTTGGTTCCCATTAATGCTCTAGCTGAATCATCATTTTCCAAAAAGGGGATTAAAGAAGAACCAATGGAAACAACTTGGTGTGGTGCTACATCAATGTAATCAATCTTTAAAGGATCATAAAGTCCTTGCATAGATCGATACCTACCAATAATTTCCTTATCTAAGATCTTGTTATCGTTACTAATATTGACAAGTGAAGAGATCTCAGCAATAATATGTTCATCTTCTCTAAGCGCAGTTAAATATTCCACCTCATCAGTAATTACCCCAGCTTTGATTTTGCGATAAGGTGCCATTAAAAATCCGTTTTCATCAATCTTAGCAAAGCTAGCTAAAGACATGATCAACCCTATGTTCATCCCTTCAGGTGTTTCAATAGGGCAAATTCTACCGTACTGAGAATAATGCACATCACGGATATCTAAATTAGGGTCCTCTCTTGATATTCCCCCAGGTCCCATTGCTGAAATCCTTCTTTTATTACTCAATTCTGATAAAGGGTTTTGGTGGTCTAAAAATTGGGTTAATTGGTGGGTATTGAAGAAGTCTTTAATCACAATTTGAATTGGTTTTGAGTTGATTAAAGATTTAATAGTTAATTCCTTTTTTTCACCTTGTTCAATAACCTGACCCTCTTCATTAATTTGCTGGCCACGGTTAACTCCATCAGCAATAGTTAACTTTTCTTTTAAAAAGCGCTCCATTCTAGTGAAGCCGCTTTCTAATTTAGCAGTAATTAATTCATTAATTAACTTAACCCGCTTATTACCAAGGTGATCAATATCATCATATTTACCAATTCCATAAGGTAAATTAATCACATAACTAATTGAAGCGATGAAATCACTGAGAGTCATTGCTTTGTTCAGATCATTTTCGTTATGGATCCCTATAACTGGTACTGAAACACTAAGATTATCATTAGTGATATATACATCTATCGTTTCATAAAAGAGTGATTCTTTTGCTAAATCAACAGCCTTACCATCAGTTGTAAGTTGCATTTTATTAACAAAACTAATTTCATTGTTCTTAGCAGCTTGTTTGATTTTGTCAATCTCTTCTTTTAAAAGTAAAGTACCTTTTTTTAAAAGTAGCTTGCCATCTTTTAAGAACAAATCACATGCCAGAGTTTTTTGATAAATACGTTCTGTTAAACGTAACTTTCTAGATACTTTGTATCTCCCTGCATTAGAGAGGTTGTACCTCTTGTTTCTAAAAAAACGCGCACATAAAAGTGCATGGAAACTCACTTCATGACACTCTTCAGTGTTTTCAATATCACGCAGTGAAATAGAAAGTTCATTGACAATGTGTTTAGCAGCTCTTTCAGTAATAACACTATCCATTTTGTTTTCCATTTCCAGTTCAAGTGCAGTTAAACTAGAATCATTTGGACTTGTTTGTTTTAGCGCATTATATTGTTTCTCAAGCGTTACATATTCATTAACAAGTGATCTTAATTTCTGATCAGAAGCGATCCCTTTTCTTCTTAAATCAGTTTTACCATCTCTAAATTCTTTTAAAAGGTTTTTGATTTCAGGATCAACATTGCTTAAAAAATCTTTAGCATTGTAAATTTCCGCTTCCAATGAACGTTTGATAAATTCATTGTTTTTAAAGATTTTAAGGATCTCACGATTATTTAAACCAAACGCTTTTAAAAGTGTTGTAATAGGGAAAATCTTAGCACCTTCTCTTACCGCATCTCTTAAAAGAATTTGTACAAAAGCATCTTCAATCTTTTTTTTATTGGAGATGTATATAAGCATCACTGAACCATTAGCAGGTAAAACCTCACAAACATAACCTTCCTGTACTCTTTTACGGGAGTTGGATAGTTTTAGTTGGGATTTTGTTAGCATGTATATCCCTGGAGAACGGGTTATTTGGGAAATAACAAACTTTTCTATCCCATTGATAATAAAAACCCCATCATGGGTTATTAAAGGCATGTTAGCTAAAAATACACCATTTGTATTGCTAGCAATATTTTTCTTTGATTTCCGTGCTTTTTTAACTGTTCCTTTATCATTATCAACTAATTCAAGATCAGCATAAATACCAACTTCATAAGTTTTAGATTGAGCACGTGATTGTGCTTCATCACGTTCTGGTTCAGTTCTGTGTAATCCCCTAAAATTGATAGTGTAGCGATCATTAGGGGATTTGATTGGAAAATATGAGGCAATTAATTTTTCAAGATCATGATCTAAGAATTTTTGGTAGCTTTTAATCTGAATATCAGCTAAATTTGGTTGGATAAAATTGGTCTCAATTTTGCCATAATACCTTCTGGTAGCTGTAGGGGAATAACGTTTTTGAAAGAAATTAGATTTTTGTGACATTTGTGGGGTGAAAAGCGGATCAAAACATTAAAAAGCCACTAACAGCGTTATAAAACGCATGCTAATGGACTTAATCACAGTTCGCTAAATTATATTAATTTTTTAACTTGTTCAATAAAAGGCTTAAAATCAAAGTTCTCATGATAACCGTTGACTAAAACTGTTCCTTTAACATTAAGACCGCTAAAACTTAAAAATTTATCTAGTAATTCTAGAACAGTTTTATCCCCACCCGCAGTTGCTGGAGTTACACAACAAATAACTCCATTAAGACCTTTTAAAAATTGATTTCTACCATACTGCTCATCACGAGTCATCCAGTCAATGATGTTTTTAGCAAAAGCAGGGATAAAACCGTTGTGTTCAGGGGTAACAAAAATTAAATTGCTATGTTCTTTGATTTTTCTAACTAAAGTTTTAATTTTGTCAGGAAAGTTAGTTGCTTCTAAATCAACAGAATAAAAATCAACTTGATAGTCTTTTAACTCAATCAATTCACAACTTAAAGAATGTTTTAATTCATTAGCAAATTTCCTGTTAATGGAATGTTGCGAATTGGAGAGCATTAAAATTAATGACTTTTGATCTTTTGACATAAATTAACACTTAAATTTTAAATAAAAAGTATTTAAAAACAATTTAATAGAAGCGTATAGCTATTTTTTTCAATGTTGTTAATTATTGAGAAAATAGCAACCTTGATAAGTCTTAATATCAGCAACCTTAGTATCTAAACTAACGGTTGGTAAATTGGGGTTTTGTTCTTTATTTCCTTCAAGTAAACAGATACAATTCTCATCAAAATTAAAACCTTTCATTATTTCAACAATTAAGTGGAATTCCAAATCAAAAATCAAAGCATCAACTTTTAATTTAGTAGGAGAATTTGAAAATACTTGTAATTTGGAATAATGCAGTAAAAACTGCTGATTAAAATCTTTAACAGTTTGTTGTCAAATTAAGTTCTTTTGCTCAAAGTTAGGATGCTTAATGTTTTCTAAATTTTTTAACAAGTATTTATGAGGCAAGATAAAATGGCTGGTGATTTTTAACTTTTTTTCAATTGTGAATCTGTCTTGAAAACTAGTGAAACTAAAATCAATGTTACTTTTTTCAAAGTTAGTTTCATAACCAAACACACCGTTTAGTTCAACAAACATTACGTTATTTTTAGACTTGTGCTTTTTTTCTAATTTTCTAACTAACTTAAAAAACTTATTTTGAAATGATCTTAAAGAATAAACTTCATGTTTTATTTTTTGTTTTTTAATTTCTTTAATTAGCGAATCAGAAAGCTTAATAGTTGTTTTTTCTTCTGGTGAATTAATTAATAAAAAAACTATTTTCAAACCCTTAAAAGTTTTCAGTATTTTTAAAAACTTATTAATTTCTGTATCTGTATTTTTGGTTTTTTCAACAAAAGCAAATACAGTATCGTTTTGACTTTTATTTCTTAATGCTAAACCAAATGCACTTCACAAGTTATTATCAAAACCTAAAAAAGGGAAGTTATCAAAATAAATCTTTTGATATATTGGAAGACTTACTTTGTTAACAAGCAGATAATTTACTTTTTTAGATCTAACTAAAAAATTAACAAAATCTAGATATTGTAATGTTGATAAAAGTCCAACCTTATTGCTATTTTGAAAAAAATCATAAGCTAATAGGCGTTGCTCATTAACGAGGGTTTGATCATTAATTTTATGAGACATTGTTTAATAAATTTTACTTAGTTTATTATGATTCAGGATATCATCCATTGCTTTACAAAAGCTTTCATATTTACTATTAAACATTTGGTGTTCACTGTTTAAGATAACAGATGATTGCAAATTATTGTTCTTAATTAGGTATTTATTGAAATAGCTTAATGTTAATTTTGTTGGCATAAATAAATCATTTTGAGCTAAAACCAAAAACACTGGTAACTTTAAATTTTTATAAGTCTTATTTAAAACTGGTAGTTCTTTCAAAATATTATTAGAAAGCTTTTTAGCCATTTTACTGTGTTTTGACAGCTCATTTTCTCAGCTGTTTTCATCAGTTAAATTACTAAATTTAGTTTGAAACATTGCTTTAAAATTAGCCATTCTTTCTGCCTTATCTTTTTTAAAAGAACTTTTGATTTTGAAATATCTCAATAAGTTACAGTATGAAAGAGGTGCTAAAAGTATGAGTTTTTCAACACGTTTAGCATTTAATAAATTCATGGCATAACTACAAACAGCAGCACCCATACTGTGTCCTATTAGAACGATGTTTTCCAGATCCTTATTTTCTATAAAAGCAGCTAATAGTTCTCCATAATGGATTGGAGATAATTCCTTATAATCAAAACCATTTACATTATGACCAGGTCAAAGTGGAGCGTAATAGTCATAATCAGTAAGGGTTTTATAAAAACTTTGAAAGTAGCTATGAAAAACAGAAAATCCATGTGCAAAAACAACCGTTTGTTTTTTTGTTTTTTTTGCTGGCTGAAAGAAAACTTCAAGCTTAGAAATATCAATCTGATTAAGATCTGAAAACCTTTTCACAATTATTTTTTTAACACACTTTAAAACAACGTGAGCACAAATTGTGATCTAATTTTTGCTTAATGATTGTTTGAAAATTTCAGCATCTTTCACACATTGTTTTTTTAGTTTTTTTAACTTTAATTTCATTTTCATTATTTGTTAAATTAACACTGTTTACATTAAGTCAAAGTGCGAGATTATCCTTTAATTTTTTGGGTATTTTTTTATTAATTCCAACGGTTAATTCAATTTGATTATTTTTAGAAATCAACCCTTCTTTTCTTAGCTTTTCAATTTCTTTGAAAGCAGCATTTTTAATACTAGTAAAAGTTTTATAGATATTTTCTAAATTCTTAGAGTTAGCAACTTTAAAAACAGTCGGTTTTGTAAAGAGGTTCACACTTATTGGTTTTTTATTGAATGAATAGTTTTTTCAAGCATCTTCTGCAGTGTGGGGAATAAAGATATTTAAAAAACTGATTAGTTGTGTAAAAATATAGTTTAAAACAGCTTGTTTAGCTAAACGATTAGGATTATTTTTAGCATCACAATATAAGGTGTCTTTAATTATTTCAAAGTATCAGCTTGATAGTCATAAAACAAACTTATTAATCACTTTTAGGCAACCTAAAAAATTATATTTCTCTAAAAATTTCTCAATTTGTTCTACTAGTGAATTAGTTTTATGGATAACTATTTTGTCTTCTAGTGAAAACTTATAATCATCCATTGATGTAAAGTTAAATCCATTAATATTACCCAAAATAAAACGTAGTAAACTATTTCTAATTCTGCGGTATTGTTCAGCAACTTGTTTAAGAATATTAACACCTATTTTGTTATCAATTTGTCAATCAGTATTAGCAACTCACAACCTTAATATATCCGCTCCATATTGATCACAAATTTTTAAAGGATCAACTATGTTTCCTAATGACTTTGACATTTTATTGCCATTTTCATCAAGTGTAAAACCATGTGAAACAAGTGATTTAAAAGGGATTAAATCATTTTGAATAATTCCGCAATTTGAAGAAGAGTTGAACCAACCCCGATATTGATCAGAACCTTCAATATAAAGATCAGCTATTGAACCATATTTATTTATTTCCAAAACATTATAGGAAGAACCTGAGTCAAATCAAACATCTAATGTATCAATCTCCTTGTGATACTTAACTCATTTTTTGGTTTTATCAGGTTTTAAAAAACAAGTTACATCTTTTTCAAATCAACTATCAATACCATGCTTTTTCAATTGTTTAATTGTGTATTGAATTGTTGAAAAATCTAACAATGGTTTGTTATTTGCATAAACAATTGGTATAGGCAAGCCCCATACTCTTTGACGTGAGATACATCATTCATCACGTTGTAAAAGCATCTCTTTTAATCTCAATTGATTTTTTGAATTTAAAAAATTAACTTGATTAATTTGTTTTTTTAACTGCTTTTTTATTGATTTAGTTTTAATGAATAATTGTTTGGAAGCACGGTATATAACTGGAGTTTTTGAGCGTCAATCATGTGGTTCGCGGTGGGAAATAACTTCAGAAAAAATAAAGCTATTGTTTTGTTTTAAACGATTAATAATTAGATCATTTGCTTTAAGATAAAAACAATTCTCAAGTTCTTTATCATTAAGTAAGTTATTAAATACACCTTTCTCATCAATAGAAATCAAAACTTCTTTAATCTTGTTTTTTTGACAAAGATAAAAATCATCAATTCCAAATGCAGGGGAGCTGTGAACAATACCTGTTCCCTCATTATCAACAACATGTATTCCCATTAGAACTGGTAAAACCTTGTTATAAAAACAATGAGAATAGCTTGAATTTTTTAAATTTGAACCCTTGAATTTTTTTAGTTTAATTGCATTTGTTCAATTTAACTTATTTGTAAAAACTTCAAATAATTTTTCCAAGATAACAAATTTTTGTTGGTTATATTCAAAAAGAAGATAATCAAAATCAGGATGAATGGCAATTGCTTGATTAGTTGGTAGTGTTCAAGGAGTTGTTGTTCAAACTAATAAATTAGCATTTTCATCTAAAAAATCACTTTTAGAAACTTTAAAAGTTAAATAAAGTGCAATTGAATTAACTTCTTTATATTCAATTTCCGCTTCAGCAAGTGAAGTTCTTGAAATTGGTGATCAATAAGTTGGTTTTAAATCTTGAAAAATGAGCCCTTTTTTAATTGCTTGTAAAAATAGTTCAAGTTCCTTAAATTGAAAACTCTCATCTATTGTGTAATAACAGTTTTGAAAATCATTTAAAAGTCCCAGTCTTTGAAATTGTTCTTTTTGAACTGCAATTTGTGAAAGTGCAAACTGATGACATAATTTTCTTTTTTCAACAGTTGAAAGATTGCTATAACTACTAGGGTTTTTCTTACTAACTGCATGTTCTATTGGTAGTCCATGACAATCCCAACCAGGAATAAAAACAACATCATATCCTTCATATAACCAACTACGTAAAATGAAGTCTTTTAAAATCTTGTTAAGAGCATGTCCCACATGAATACTACCATTTGCATAAGGTGGTCCATCATGCAGTATTTTTATCTGTTTTCCTTTATTCTGTTTTTTTAATTTTTGAAAGACTTTTTTATCTTTTCAAAAATCATGAAAATTCTTTTCACTAGTAGATAAATTTGCCTGCATCGCAAAGGATGTTTTAGGCATTAACAATGTCTTTTTTAAGTCCATTAAATCAGAGTAATTTTATTAATATTTATCTAACTTACATCTGATGTATAAATCAGTAATAAACATAGTTTTATTTTGTCCAGAAATTCCTAATAACACTGGCAACATCGTACGTAGTTGCACTGCTTTTAAAGCTAATCTACACTTAATTAAACCTTATGGCTTTTTCTTAAATGAAAAAAGGATGGTTAGAGCTGGTTTAAATTGTTGAGATAAAATTCAATTATTTGAACACAAATCATGAGAACATTTCTTACAAGCAACCACTGAAAATAAAACTATTTGGCTTTTAACTAAAAGTGGTGATAAAACTCCTGATCAAATTTGCATGACAAATAAATTACCAAACGAACTTTACTTTGTTTTTGGTCAGGAAACAAAGGGATTACCTAAAACAATCATGGATAACTTTAAACAAAACCAAATTAGAATTCCCATTTGAAATAGTGTTAGAAGTATTAATCTTGCTAATGCAGTTGTCTGTATTTTGTATGAATATTCAAAGCAAAATCAATACTCTAATTTAGATAAACAGTGCGCTTAAGAAAAGTTAAAAACGCTCTTTTAAAAATTAATCAAAGTCCTTATTTTTATTCAAAAGATAAGTTTGCTAAGTTTACTAAAAAACAATTAGTGCTGGAATTGGGTTGTGGTAAGGGTACTTTTTTAATCAAAGAAGCACAAAAAAATAACAATTTTCTTTTTATAGGAATTGAACGTGAACCTACAATTGTTTTAAAAGCAATTAACAAAATTAACAAGTTGGATTTTAATTTGGAAAATATCTTATTGTTGTGTACAGATGCAAAACAACTTGATGATTATTTTCAAGCTGAATCTGTTCAAAAAATCTTTATTAATTTCCCTGATCCTTGACCTAAAAAGCGTCATATACAAAGACGTCTAACAAGTCCAGATTTTTTGAAACTTTTTTGAAATTTACTAGTAAAAAATGGCTTAATTGAGTTTAAGACTGATAATGATAAGTTATTTGAATATACTTTAACAACATTGCAAGAAAATAGTCAAATTTTTGAAATTATCCATCAAATAACTGATCTTAACAATTCTGAATTCAGTTTTCAAAATAGTATTACTGAATATGAACAGCGCTTTATGGAATTAGAAATTCCAATTAAAAAACTAGTGATTAAGAAAATAATTTAATTAAAGTTATTAGAATAATTATTAGCTTGATGGCGATTGTGGCGAAGTGGTTAACGCACCTGATTGTGGATCAGGCATTCGTGGGTTCAATTCCCATCAGTCGCCCCATTAAAGATTCAAAGAGCAGCAGTTAGCTGCTTTTTTTATTAGCTAGTTTAGTGGAGTATAAACACTATCCAAGAAATTTAAAAAATCAACACTAACTCTTACTGCGTGTTGTTTAATTCCATAAGATTTTTGTGATCTTTGAACTGCTGGTTGAGAAGATTGTGGTGAAGTAGGAGTAGTATTTTGTCCATTACCACTACTTGAGTCACCACCTGAACTGCCATCACTACCGCCCCCTTCACCACTTGAAGCTGGTGGGGTTGAATCACCCTGACCTGATCCAGGTGCATTAGGATTTGTAGAGTTATCCAAACCATAAAAATTAGCTCTTATCTTATTAAGGTTATCTTTTCTAAAAGCAATCACAATTGCTTTAGTTACATCATTACCAACTGTTTGAGCATGCACATTTGATTGATTGATTAAGTTAGCTACATTACCACCATAAGTACGTTGAATAAAATCAACTATTTCTCTATAGCGAACTGCAGAAGCATCTGATCTTCTTAACTTATTGGAATTGTCTTTATCTTCTTGACTTGCAAGTTCGTTTTTTCTATCCACAACAGGAAAACGTGCATATGGGTTTCACTTTACTGAACCAGGAGCAATATCATTCCAAATTAAAAATTTAACTCCGTTTGGATAAGTAGAATAACGGTTTTGCACCTGTCTGATGGTGGTACCCAAGGTACCTCCTCAACTAAGAGAACTTGTACCTTGATTAGTAGAACCGTTTAGAAAATTAATTTGGGAACTATCTGTTTCGGTTGCAATAACAACCACATAATTACCATCGTTATATGATCTGGTTCCAAAAACTAAGTTTCTAAATGTTGTACTGTTAACAGGCAATTGGTTGTAAGCTGTATTTTTTTCCACACTTTCAACAGAAAGTTCACGAAAAAAAGACTGATCGATGTTACTACAAGAACTTAAAATCATTCCTGTTCCTAGTAATAAAACTAACCATGCTTTTTTATGTTTTAGATTTCAAAAAAGACGCTTCATTTCTAAATTGACTGGTGTCGGAGACCAGACTTGAACTGGCACAGTCTTTAACGACCACAAGCACCTCAAGCTTGCGTGTCTACCATTCCACCACTCCGACTATTTGCTTATAGTTAATATTGTTTATATTAATAAAATTTTAATACAAGAGTTAACAAGGCTATTAGCTTGTACTAGCTTAAACTAGGAATATAAAGGTGTTATTTCAAGCTAAATTCCCGCTTTTTTCTCCTTAAATTCAACTTTTTTTATCCGATTATTGGTAAATAAATCAGTTTAAAAAGTTATTAACAAAATGTTTATAAGTCTCAAAAAAGGGTTGTTTTAGACTGTTTTTAATGTCAATAAGTTGTAAATAAATGGCAGAAAAATCTTTTTTTAGCCTTTTAATGCAAAGTAATTTCAATAAATGGAGGATAGCGTCTTATACTCTTTTTACTTTTGAACGTATATGCAACCCAACTATTACCGTGTGATCAAAAAAGCAACATCATTTTCTGGATTAGAAATGATCTCTCATGCTTATGGTCAAATTGCGGGAGTGGAAGTTGTTGGGTTTTATTTATGACTAATAACAGAAGCTAATATTCAGGCGTTCAACAGTGAAATTAGAACCCCCATTTCACGATTACAAAATGCCTTTAATCCTTTTGAAATTAAAAAAAACCAAGTCCCTGAATGGATTTATAAAACTATTAGCAAATTAGAGAGTCTTGGTTTAGTGAGAACTTTTTTTTCACCAAAACGCTCTGAAATAACTTTTTGTATTATTGATCCTTTAGATTGGAAAGAATTTAAGCAAAACAAACAATTAAAAGAAAAACTAGTTGAGGCAATGGGAAAAGTTGAATATGACCGAAACTGCTTAGCTTTTGATCAAATCGACAATCTTCAATTTGATAATGCGCTTGAAATCTCTGCTAACTTTGAAGTTAATTTCACTGCAAACCAAAGCGATGTTTGATTTAGCTTTAACTTCGAGGAACTACATAAAGAACTTGTAAAAAACAAACTTTTAATTTCTTTAGATGAAAAAGCTAAGACTTTAATTAATGGTTATTTTGAAAAATACAAGCTTTCATTGCAACAAATTACTGATTGCATCATCAACAGTACTCAAGAGAATGAGCTTGATTTTCAAAAGTTAGAAATGATGTTTTTTCAAATAGTAAAAAATGATACAGCTCCCATTTTAGAAACAGTTTCAAACAACAAAGACTTTTTTTATAAGAATGAAATTTTAGATGAATCAACAAAAAAAGCAATAACAGACTGTCATGTAAACTTCAATTCTGAAAAATACCTTTTTCTTCTATATGGAAAGATAGATGAATCGCAATTGCAATTAGTTAGACAATTGAGAAGTGATTATCAACTATTAGATAAAGTAATTAATTTAGTATTAGACTTTTCCTTTTGAAAAAATAACGGAATGTGAAGAGAAAAATACATTCTAAAAATTGCCCAATCCATCAAAATTAATAACAGTCAAAACAGTTATGAAAAAACCCTTAATAATTTTATTAGAGCACTAACTTTAAATAAAAAGCATTCTTTAAATAATATAAAACCAGTTGAAAAAACAATTTCCTTTACTGAGTATTTTGAATTTATTAAATAATGAAAATTTCACCAGATCAAAAAGATAAAAAGTCATTATTTAAAAAACATTTTGCTTATTTGAAATTAACTGAAAAAGAACTTAATGATCCTAAGATACAAACACTTTTAGAAGTTGCGTGGAATCATTTTGATCAGTGTAGAAAAATTAAAGAAAGAAGATGTAGTAATAAAGGTGATATCCATCTTCAAGCTGTTAGAGAGTGGTTACCTTGAGAATTTAACGCTAATCAACTTAAAAAAAATAGTACAAAAGAAACAACGCAAGTTAGTGAAAATGAATTTATGGGTAATGTTATGCTCATGCAAACTATTTGTCCTAAATTAGTTAATAAAGCAAACTGATTTGATTTAACTTATGAGCGTTTTATTGTTACAAAACCAAACTGATTTAAATACATGGATTTGATTCCAATGATCCAAAATTTACCAGTAACTCCAAGTGATAAAAATAGTTTTGGTTATGCATACAAAAAGATATCTAATCTTTTTGAAACTGAAAAGAAAACAAAGAAAAGGATATTTTTTTTCAATTTACAAAAGAATAACATTAACAATATGGCAGCTTGTATGTTAACTTGTGAAATAGCTAAGAAAAATATAAAAGTTGCTTTAATTTATTGTGAAGAATTTGTTAGCAGATATGACAAAAGTTATTGAAAGGTAGATGATGATTTAAATTTACTTGATGAGGCAAAAGTAATCATATTCATTGGTTTAGGTCAGGAAAGTTTTCATAACAAAAACTATATCTTGTTTATGACAAGGCTTTTTATAAATTGTTTTTTGAAAAGAAAAGATGTTTTCTTTTTTAGCACTACTTATAGTGATGGTAATGGATTAATTCAAACATTTAAAAACCAAATTATTAGTAGTGCAAATTGAGTAAAACACTTTTTTGAACAACTTAATGATTTATTAATGATTAATATTTAAAAATTACATTCTTTCACCAATAAATAGCTAAGTTTATGTAAAATTTATCTTGTATTACCTGGAGTGGCGGAATGGTAGACGCGGTGGACTCAAAACCCACTAGGAAACTGTAGGAGTTCAAGTCTCCTCTCCAGGACCATAATTAAATACTTAAATTTACACTACGAAATTACAAGCTCAGTTTAAAGCTGAGCTTGTTTTATTTTTATAGTGATTTCTATTTAGCTAATGCTCTTTTTCAATAAAACTTTTGAAAGGAACGCATAATTGGCACTCCAAAACAACTAACAAGTCCAAATTTCACCAAATTAAAAAACGAATATAAACAGAAAATTCCCAGCCAATAGTTATTAATTCCAAAGAAAAAGAAATGAAAATCAGTGTTCTTGTTATATTGTTCAGCAACTATTAAAAAGTTAGGTGTTTGAACATAACCAAAGTAGTATCAAAACAAAGGTGTTATCAAAATACAGTTAAGCGTTGTTAGTAATAAAGCATTGGATAAGGTTGTAAATAAAAACACAAAGATTCAACAAAATCTTCTTTTTGAAAATAAACAAAAATAAAGCCTTGTAAATAAGATAGTCGATAAGTTAGCTAAAGTTACAGTTAGAACACCAATAACATTATTAGTATTTCAAAAAAAACTAGTTAAAGCAGAAGCTAATGTAATTGTTAAGCTTCAACCTAGTGACACAAAAAAAGCACATGCAACTAAAAAAACAACGGAAATATCAAAATTTAGTTTTAGTTGTGAGAAAAAAGGAATACTAATAAATTCACTAATTAATGATGTAATGATGGCCAAAGCAAGCATCATTGCACTAAAAGTAATATTAAATAACTGATGGTATGATTTTTTTGGCAAAAAAACCAGTTTATTACCATTCATTATTTAAAAGACTCTTGAATTATTAGTTAATAATAATATTTATTGATATGGACAAATTTTTAATTGATGTTATTGTAGAAATCCCTAAAAACAGCAAAATAAAGTATGAGTATGATCGTCAAACTGGTCAAATTCGCGTTGATAGAATCCTATTTGGAAGTGAATCATATCCACAAAACTACGGTTTTATTAAAAATACATTAGATTGAGATGGGGATGAACTTGATTGTTTTATCTTTGCAGATCAACCATTTTTGCCTGCAACAGTTGTGCCTACAAGAATTGTAGGAGCACTTGAGATGATTGATGATGGGGAAATTGATACTAAGTTATTAGGAGTTATTGATTGTGACCCTAGATATAAAGAAATTAATCAAATTAGTGATTTACCTAAACATAGAATAGAAGAAATTCTTATCTTTTTAAAAACTTATAAATTACTTCAAAAAAAGACTGTAATTATTAAGGGTTTAAAAGATGTTTGTTGAGCTAAAAAAGAATATGAAATTTGTTTGCAATTAATGAAAGATTATGGTCATTTATCAAAAGATCAATTTATCCAAAAAATGCAAATTCTTCATCCAGAACATTACCAAAAGTAAATATGATAAATGCCAATCGCGGAATGCTTTTAGAGACAATTGTTAATCAAACAATTGCTAGGTTAAAGGATCATCCAGATATTTGACTGGAAAAGCGGTTCTTACCTATTAAGCCTATAGCTTTTCGTCATGCTCATGTATCTGGCAACGTATCACAAAAATCAAAAACTGATTATTATGGAATTTACAAGGGAATGTATTTTGATTTTGAAGCAAAACAAACAAATAAAAGTAATTTTCCAATTGCTCAAATAGCAGAACACCAATTAAATCATTTGAAAAGAATTGACCAAATTGGAGGAGTTAGCTTCTTGCTAATTTACTTTCAAACTAAAGATCAAATTTTTGCATTTCACACTAAGGATCTTCTAGAAACAATAAAAAATCAAGAAAGCAAAACAATTAAAAGGGAATTGATAGAACAAAAATCTCAGAAAGTTCCACTATTGTATCCTGGTATTATTGATTTAATTTCAATAATTCAAAGCTTTAAAAATTATTAGTCTGCGTACTTTCAACGCACTTTAGCTGCAGTTTCTTTGATCGTTTTGCTTGGACTGTAGCGCACTAATGGTTTTGGTGGGATATGTCTAATCTCACCTGTTTGCATATCCTTCTGATAACGTGCATTTCTAATAGTAATCCTTAATTTACCTAAATTTTCAGGTAATATACAAACACTTCTACTTACCAATTCATTTAGTAACAATGTGTTAAGGTATTTAAAGATTTCCTTAATTTTTTTTTCTTTTATACCAGTAGCAACTGCAATTATTTTATTGATTTCACTACGAGAAAGTGGCTTACTTGTATTTGATGTTTTTTCCATATAATTATTTTTTAAAACTTTATTAGCTCTGTTATTGCTAAAAACAAAAGAGAAACAATTTAACGCATTCTATTTTATTATTGATTCAACAATTAAATTAAAACACTTATAAAGTAAATTTTTAGACAATTATTTCTATTCTTAAGACATGGAACAAAACAATATTAAAGAACAACTTATTTCTTTCTTTAATCAAGCATGTTCTACCCACCAAGAAAGACTTGATTTTATCTGTTCTACAAGAGAAAGTGATACTTTTTCTAGTGTTGATGTACCACTTGAACCCATTAAAAATATTATTGAAATAACTAAAGATGAAAATCAACAAATTGAAATTACAAAAATAGCTGTTAATAACATTAAAACATTATCTTCTGTTGGTGCAACCGGTCAGTATATGGCATCTTTCTTTTCAACAAATAGTGAGCCAGCTATAATATTTTGCGTCATTTATTTTTTATATCACTTTGGTTTTTTAAAAGATAACAATAAAAAACAAATAATAAAAAAGGCTTATGAAACTATTGCTGATAACATTGCTGACTATTTAAATGAAAATTAGGTTTAAGACTTATTATTTTGTGTAATGGTAATATTGTCTTTAACAACTGCAATGTTGTATCCAACACCTTTTTTTATCTTTCCCTGAAGGATATATTTAGCTATTAAAGTTGCAACACTATGATCAATAAAGCGCTTAATTGGTCTTGCACCAAACTGTTGATCAAAACTACTTTTATAGATAAACTCTGTTAGATTTGAATCAAAATTAAAAAATAAATTTTGTTTATTCAGGCGTTTTGAAAGTTGTGCCAACAAGCTGTTGATTATCGATAAAACTGTATCTTTCTCAAGAACATTGAAAAATACTATCTCATCAATACGATTTATAAATTCAGGACGGAAATGTTTCTTTAGACTTTGAATGGCCAAATCTTTTTTTCCTTCTAAAAGAAAATTTGAACCTAGGTTAGAAGTCATAATTATCAAAGTATTTTTGAAATTAACAACCCTTCCTTGTGAATCTTTTAAAGTACCATCATCTAAAACTTGTAATAAAACATTAGTTACATCAGGATGTGCCTTTTCAATTTCATCAAATAACAAGACGCTATAAGGTTTTCTTCTAACCGCTTCAGTTAGCAAACCTGATTGTTCATAACCTATGTACCCTGGGGGTGCACCAATTAATTTAGCTACTGAATGTTTTTCCATATATTCACTCATATCAAAACGAATCAGAGCTTTTTCATTGTCAAAAAGAACTTCTGCTAATGATTTGGCAAGTTCAGTTTTACCAACACCAGTAGAACCTAAAAAGATGAAAGAACCAATAGGTTTGTTTGGGTCATTTATATTTACTCTACCTCTAATTACAGTGTTAACAACAGCATCGATGGCTTCATCTTGTCCTTTAACTCTTTTTTTGATTTCATCACCTAAGTGCAAAAGTTTATCCTTTTCACTTTCTAATAGTTTTTTAAGTGGAATTCCTGTTGTTTGTGAAATAACTTCAGCAATTTCATTTTCAGAAACTTCAGTTTTAAATAAATCGTGCTTAGAAGTTGCATATTTTTGTTGTGCACTTTCCAGTTCTTTTTTAAGTCTTGGGATATCAGAGTACAGAATTTTAGATGCACTTTCATAATTTCCTTCACTTTGGTATGTCTCTAGTTTGGTTTGAAATTCTTCAATCTCTTTTTTGAGCTTATTAATGTTTTCAAAATCAGCCTTTTCCTTTTTTCATTCATTTATAAGTGAATCACGCTTTTGTTTAAGAGCATCTAATTGTTTTTTTAGTTTCTCTAAATATTCTTTCTTTGATTGTTTGTTATCATTTTCCTTATCTTGTTTAAGAGCTGCATACTCTGTCTCAAGATTGATTATTTCACGTTTAAGACTATCTATTGCAACTGGTTCAGATGACATTTCTGTTTTGATCTTAGCAGCAGCCTCATCAATAAGATCAATGGCTTTATCAGGTAAATTACGTTCATTAATATAACGAGTTGACATTTCAACAGCAGCTACTAAAGCACTATCAAAAATAGTGATGTTATGAAAGAGTTCTCAACGTGTTTTTAAACCACGCATAATTGTTAGTGCCTCTTGACTACTAGGCTCGTTAATAAGAATTTTTTGAAATCTACGTTCTAAAGCGCCATCTTTTTCAATGTATTCCCGGTATTCTTTTAGAGTAGTAGCGCCAATTACTTTTATTTCTCCTCGAGCTAGCATCGGCTTTAATATATTGGCAATATCCATTGCACCACTGCTAGAATTACGTCCTAATCCAACTATTTGGTGAATTTCATCAATAAAAAGAATAATCTTGTCATTTGATTCTTTTACTTGCTTAAGAATGGTATTAATTCTTTTTTCAAATTCACCTTGGAATTTAGTGCCAGCAATTAATCCAGAAAGAGATAGTTCATAAATTTCTACATCCCTTAAATTTAAAGGTACATCATTACTAACAACTCTTCTAACAAAACCTTCTACTATTGCGGTTTTACCAACTCCAGGTTCACCAATTAAAACAGGATTGTTTTTGCTTTTACGACTTAATATCTCAATTAAACGACGAATTTCGTTATCTCTTCCAATAATAGGATCGACCTTATTTTTTAATACTTCATCGTTAATATTACGACCAATTTCTTGCAAAAAATTACGATTTTCACCAGCAGGTGTGAAATTAATATTCATTTTAATTGCTCACTCCTTTTAATGCAAAAATTATATAAAATTAGCACTCAAGGCATTCGAGTGCTAATTAAAAACTAGGTTTTTTGTTTATCTCTAATTTATCTAATCAGAGTTGATATTTTTGTCAAAATAATTAGAAACCTAAAATAACTATTAACTATTTTTTTGTGCTTGTTTTCAAATTGAGGGAAATAATGCTTTTAAAAAGTATTCTAATCGTTTTTCAACTCATTTTCGATAGTTTAAAATTGCTTCTGTTTGGGGCATAATCTCAGTTCATTGAAAAGCAAATAATAAAACATAAATAACTGTTTCAAAAACAAGCTTACGCTTAAGTCCACCATAAATATCAATAAGTTTATTTGCTGTTTCTATGGTTAGTTCTTCACGAATAATATTAGCTATTTCATAATAAGGATTATCAACACAAGATCATTCAAAATCAATAAGGATTACTTGCTTTTTAGGTGTTCAAACCAAATTATCAAAAGTAGCATCATGATGACAGAGAGTTTTTGGTATGTCAATATGCTTCTTAGTTAAATTAAGATATAACCGTTTATAAAAAGCGGAAAGCTTAGTTTTTTCAAAATAAACCAAAGGATCAAATATTAAAAGTTCATTAGCAAATGGTTTTCAGTCAACTGAATGAACTTTTTTTAAAGATTCAACCAATCTGCATAAAAATAGATCAGTTGCTCTTTTAGGTTGAGTGCCTTCTATCCACCTTTTTATAGCATTACCTGTTGTTACATCAAAATAAACAAATGGGTTTGAAAAAAAAGGTTCTACTAGTTGCAAAATAATTTGTTCATTTCTTCTGTCTAAAATAACATCTTTTCTTGAAAGTCTTATTTGAAATTTACTATGATCTTTCAATTCACAAAAAAAAGAAAAATTAGTAAAACCAGAATGAATTTGCTCTATCTTGAAAATATCTGTTTCTACAACTTGAAGTTTTTCACAAAGAAAAGCAACCGCTTTTTTTAAATCCATTAATTTTGATGATTATTTTTTAATGTTTGTACTTACTCTCAAAGCATCTTCTACAATCATCAATTCCTCATTTGTACGAACTCTAAAAATTTGATACTTGCTTTCATTTGTTGAAATTAGAGAACTATCTTGATAATTTCCAAATAAATTACTGTTAGTTTTAAAACCAAGTGAAGCAACTTTTTCAATTATTAATTGCACACAATAACTAGCATTTTCACCAACTCCCCCAGTAAATACCAAGCTATCAATTTCACCTGAAAGTTGATTTAGGTATTTAGCAATATAGTCAGCAACACGATTAACATACATTTTTATAGCAATATCATTAATTTCTGGTTTATCAAAAATATCACGCATGTCAGAACTACCTGTTATAGCAAACATTCCACTCTTTTTATTTAATTCATTTACAACATCATTACATGAAAGCTTTTGCTGTTCAGCAATGTAACTAACAATGGCAGGATCAATATCACCACTACGTGTTCCCATTATTAATCCTTCAAGGGGAGTGAATCCCATCGATGTGTTTAGTGATTTGCCTTGTTTAATCGCACAAACACTTGCACCATTACCAAGATGACAAACAATTAAATTAAGTGGTTTTTTATTAAACTTTTTTTCTAAAAACTCGTTAATGTATTTATAAGAAGTTCCATGAAAACCATATCTTCTTACTAAGTTATTTTTCTCTCAATTTTCAGGAACTGCATAAAGATAATTTTCCCTTGGAATAGTAGTGTGAAAAGTGGTATCAAATACAGCAACATTCTTAGCAGTTTTTATCTCTTTTAGAAAAATTTCAATAACATCTGCTTCTGGTTTATTATGAAGCGGTGCTAACTTAATGAATTCTTTTATTTTTGCTAGTGAATGTGTATCAACAAGAACTGCATCTGTAAAATAATTTGCTCCTTGTACTACTCTATGCCCTATTAGCCCAATTTCTGAAAGTTCAGTAATAATTTTGTTTTTTTTAAGCGCATTTAAAAAATGCTTAACAGCAAGATTATGATCATTAAATTGAACCTTTTCTTCTATCTTTTTTTGATTAAATTCAAGCTTAAAAAAACCATCAATGAAAATACGTTCACAAAGTCCTTTAGCTAGTACTTGTTTTTTATCATTAAAAAGTTGAAATTTAATTGAACTGCTACCTGCATTAACAACCAAGATTTTGTGACTTTGCATTTAACCGAAGAATATTTACCTTATTTATTAAAAATAATACTAAATTAATATAGTGTGGAATAATTAAATGTTTTAACTAAAAAGAAATATTATTAAATCTTAAACAATGAAAATTAAAAAATGCTATATTATCGCTGTTATTTCAGCTTTTATTGGTTTTCTTTTTTACTTTTTATCTTCACTTATAGGGATAACAGATGTTAATAATGCAAAAACAGTAGCAACTGTATTTTTTGTATTAGCAACATTAATAGTTGTTAATGCTATTGTCTTCTGGATTCTTTATCTATTCAAAAAAAAGAACGTAGTTAAGTAAATTTATTTGTCACTAATAGTTTTTTAGCACATTAATTTGATAGAAAACAAATTGAATGTAATATGGAAACTATCAGAGAAACACTTTCTTCTATACAAAGAAGCAATTTTTTATTAAATGAAAGCGCACAAATTCTCAATACGTGTGTAAACAAGAACACATTGCTTGACGATAAGCTGCGCAATTCGATTAATTTTGCACTTAGCATCATTAAGCTAGCAGACTTTTGTCTAAAGGATGATACTGAAAAAATTAATCAATTCTTAGGTAAAACATATGATTTACCATGAGAGTTTCAATTTAACCAAAAAGACAAGCATATAGAATGCACTGTCAAAGACAATTTTGGTCGTGAGAAACTAATAAATTTGATTTTTCAAATTGGTGATGCTATTGAAACTTATCATACTACTTTAATAAGATTCAAAATTCCCAAGCATTGTTTGAATGCAAGGGATCAAATCAAAAAAATAATGGAGGGCAAATAAAAATGATTACATCTATCTTTGGAAAAGTTACTTTTGTAGGCAAAAGAAAAATAATTGTTGAGCACAACTGGATTTCATATTGATTTAATACAAAAGAAAACCATAAATTTGAAAAAAATTTGGAAAAAAATAAGCAAATTTTTTGTCATATTATTAAAAAAATTGTCGCTAACCAAATTATAGAAGAGGCTTTTGCCTTTAATACTCTAGAAGAAAAAGAGTGGTTCTGTAGATTAATAGAACTCAATGGTATTGGTAGTAAAACTGCACTTAATTTGCTCAATAATGACCTTGAGGAAATTAAACAATACATTCTGGAAAATAACTACAGTGCATTATGTGGTATTAACGGTGTAAATAACAAAATAGCTCGTGCACTTTTATCACTTGAAATATTTGAAAAATCTGAAAATAATAAAAATATTAAAGGAGTTCAAGTTGCTGATGGTTATGATGAATTGTTTGAAACACTAAAGTCACTTGGTTACAAACAACAAGAAATTCAGGATGCACTAAAAATGATAGAAGTAAAACCTGATTTTGATATAAGTCAGTTAGTTGCAGAAGTAATTAAATTAATGTCTTTTAAGAATAATGAAATTACAAATAAAACCGCCTAATACCTTTGATGAATTTGTAGGAAAACAAGAAATAATTAGTCAAATTCAATTAAGTATTAAAGCATCTAAATTAAATAAAACACAACTAGATCATATCTTGTTATATGGCCCACCTGGTGTGGGTAAAACTACTTTAGCCAGATTAATAGCAAATGAATTGAAAACAAAGTTGCAAATTATTCAAGGTGGACATTTACAAAAACCAAGCGATTTCTTAAACGCAATTTCACTCATTAAAAAAGGTGATGTTCTTTTTATAGATGAGATCCATGCCGTAGCACCTAATGTCATGGAACTAATGTATCCAGTTATGGATGTGTTCAAAATACAAGTATTAATTGGCAAGGATTTTAATTCCAAGATAGTTGAAATGAAGGTAAATCCTTTTACTCTAATTGGTGCAACTACACAACTTGGTAAAATCATCAATCCTTTAGAAGATAGATTTGGCGTTATCTTAAACATTAACTATTATTCAAATGCTGAAATTGAAAAGATGGTAAGTATCTATGGAAAGCAAATGAAGTTAGAGCTAAATTCAAATGAAATTTCAGCTATCACTGAACATAGTAAACAAACACCAAGAATTGCAATTAGAATAGTTAGAAGAATATTTGAACAAAAAATTGTTAATAAAAAAATAGACCTTGAGAGTTTGTTTAAGAATTTAATGATTTATAAAAATGGTCTGCAAAGTATTGATGTCCAATATCTTGAGGTTTTAAATCGCCAAAATGAACCACAAGGAATTAAGTCAATTAGTTCCATGTTAGGTATAGACAGACACACTATAGAAAATAAAATTGAACCTTTTTTGTTGCGTGAAAATATGATTCAAAAAACCAAAAAAGGCAGGATTATTACAAATAGCGGAAGAGAATATTTAGTTAACTTTTAAGCTTATTAGCTCTTTTAATAATTTCAAAACCAAAACTTTCATTAATATCAAAAATTAATTTATCAAGTGAAGATTCTTCGCTTAATTTGCTAATTGATTTTGGTATGAATTGATAAAATAAAGATTTTTTCTGTCCTTCATCTGTATCTATCTTTTTTAAATCAAAAAAACTAATACCTATTAATCGAACGTTCTTTTCAGTATTAATTAATAGTCTGTTGAATAATCGTTTTGTTATTGACAGTAGTTTTCTATAATCGTTTGAATATTTTTTCATTTTATTTGAATGAGAATTAACAATAAAATCATTGCTTTTCAATTGCACAACTATCCCTTTACACACTTGTGAAGAGAGTTGTAATCTTATAAAGAGCTGATCAAATATTTGCGTTAACTTTTTATTTAACTGATTGTTTGAATAATTTAAATCTTCTAACGTTTCACTGACTGCAAAAGAGCGAGACTTAACCTGATTGTTATTATCTGTATATCATTTGCCTAGTGAAACTGCTTTTAAAGATTCTCAAAAATTACCAAATACTTTTTTTAAAAGCGAAGCATCTTCACAAACTGCTAGATCATTTATTTTGTAAAAATTATTTTTAAAAACTAAATCAATATGTTTTTCTCCTATCCCTGGAATTTCAGTAATTGGTAGTGGTCAAAGTTTTTTTTTAATATCCTTAACTGAACAACTTTTAATTCCAAATGGTTTTGCTTGATTTGAAAAAATCTTAGCAATTAAAAAATGATCTGAGATGCCAATTGAAATTTTGATTCTCAAGTTTTGAAAAACAAAATTTTTTATTTTTTTCGCTATTAAAAATGCCTTTTTAAATGAAATGTTTTGAAAACAAGCAACCCCTTCATCAACAGAAAGAACATCAATTTTTAATGAAAAAGTGCTTTCAATAACTGAAAAAATACGTTTAGAATGTTTTCTGTAATTACGAAAATTAGAATGTGCAAAAATAGCATTTGGACATAGTTCTAATGCTTTTAAAATAGACATTCCAGATCTAATTCCATAACTACGAGCAACATAGTTACAAGTTGAAACTACACTTCTTGAAAAGCGATTACCAACTATTAAGGGTTGATTAACTAATTCTGGATTTTCTAATTCTTCAACAGATGCAAAAAAAGCATCAAAATCAAAATAAAGAAAAATTAAATTTTTATCAATTAAATATTCTGGTTCAAAATATGTAAAGGTGTTAATCATATTTATCAAGTTATGTTATTATTTACTTGCAATAAAACCAAAGAAAATAACGAGTTTTTAACTTTAATAAAGTTATCGAGGATTTATCATCGTTTGTGCTATTGCGAATATTGTAGTTAATGGTAGATAGTAAGAAAAATAAAAAACAGCAGGTTACGGATTTTTCTAATTTACTCTCTCAAAGTAAAGGATTTGTTATTTTTGACTATTCAGGAATGTCTGCTGTTGATGCAACTTTAATGAGAAAAAAGTTGTTTAATAAGGGTAGTAAGATAAAAATTGTTAAAAACAATATCTTAAGACGTGCTTTAAAAACTAGTAATTTTGAAGGTGTTGATGAATCGGTCATCAAAGGAAAAATTGCAGTTGCTGTTGGTATTAACGAGATCTTAGAAACCTTAAAAGTTGTTGATAGTGTAGTTAAAGAAAAAGAGTTAATGAAATTTGTTTGTGGTCATTTTGATAATCGTATTTTTAATAGTGATGACTTACAAAAAATAGCAAAACTCCCTGGTAGAAATGAACTTTATGGAATGTTTCTTTCAGTTCTACAAGCACCATTACGAAAATTTCTCTATGCTCTTCAGGCAGTAAGGAATGCTAAGTAAATTAAATAAATAGAAAAATATTATGGGAAAACTAGATAAAAAACAATTAATTGAATCTCTAAAAGAGATGACTATAGTTGAAATTGATGAAATAATCAAGGCTGTTGAAGAAGCATTTGGTGTAACTGCAACTCCAATAGTAGCTGCTGGCGCAGCTGGTGCTACACAAGAAGCTGCTAGCGAAGTTAGTGTAAAGGTAACAGGATATGCTGATAATGCTAAGTTAGCTGTTTTAAAACTTTATCGTGAAATTACTGGAGTTGGTTTAATGGAAGCTAAAACTGCAGTTGAAAAATTACCTTGCGTTGTAAAACAAGATATTAAACCAGAAGAAGCAGAAGAACTTAAAAAGCGTTTTGTTGAAGTTGGTGCAACTGTTGAAGTTAAATAAAGATGGCAGTACAACAACGGCGTTCTAGTAAACACCGTCGTGATAAAAGACGTTCTCACGATGCACTTACTCTACAAACTTTAAGTGTTTGTAAGAAATGTGGTAAGAAGAAGTTATCACATCGTGTGTGCTCTTGTGGTATGTACGGTGAACTAAGAGTTAAAAAAGCTCACTAATTCACAGATAAATATTATTGTTTTTTTGCAGTTGAATTTGTATTACTTTTATGCAAAATAACAGCATTTTTTGATTTTAAACGCTTAGCTCTGTTTAAAGAAATAATCCCTTTTCTGGCTAAACGATCTGCTTGAGAATAAACAGAACTGAGATTATCTAAATTAATCTCTTTATTAAATTTTTTAACATTAGTTTTTAGTTTAGTTTTTTGTCCTTTATTATTTAAATTTCTCTTAATGTCTTGACGTAATCGTTTTTCGTTAGATTTAATATTAGCCATTTAGGTTATTAATTAGTTTTAGAGTATATTATTATCTTGATTATATTGTAACTAGAACCATATATGGATGGTGGACAACAAGGGAGTTTTTTTGGGCTTTTAGTAATTGTTATTCCAATAATTTTGTTAATTGTTTTTTTTTCCAAGAAAAAAGGGGCACAAAAAAATGATTTTAGTGGTGAAGGAGGTAATCGATCATCAAGAAAAGATGAAGTGTGAAAAACAATTAAACAGTTTTTGCAAGAAAAGAATGAACGTGGTAAAGAAATTATTAAAACTTTTGTAGCTAAAAAACCAAACCCTTTGCATTCAAAAAAAGACCGCAAGCTTTTCAATCAAGAGATACAGGCATATATTACTAGTAATAACTTAGGAAAAAGCGAAGCAAAACGTTATAAAAATGAGCAAACTCGCTTAATGCAAAGAGAACTTTATTGTATTTATTTTGTTACAAAAGATGCTAAATCAACTGAAGTTGATGATGCTAGGATTATAGAAGCTGAGGTTTATCAAAAACCTACAAAAACCAAAAGTACTCCAGAGCGGCTAATTCGTATACTTGGTTTAAAAAATTTTGAAACTGAAATGCAATGAATTCAACCATTAATGGTTCGTGAAGAAAAGAGAAAAGAAAAAGAAGAACAGAAAAAACTTAAATTAGCTGCAAGAGAACTAAAAAAGAAGAAAAAGAAGAAAATAAAAAAACCAAAAGAAATCAGAAATCAGAAAAATGTTTAAAATTGTTTTCTTTGGTACTTCAACGCTTTCAAAAAAATGTTTAGAACAACTTTTTTACGATAATGATTTTGAAATTTGTGCTGTTGTAACTCAGCCAGACAAAATTAATCATCGTAACAATAAAATAGTACCTTCTGATGTTAAGTCTTTTTGTTTGGAAAAAAACATAACTTTTTTTCAACCAAAACAAAGCATAAGCATAAAAGCTGATCTAGAAAAATTAAAAGCTGATATTGGTATTTGCGTTTCATTTGGCCAGTATCTTCATCAAGATATTATTGATCTTTTTCCAAATAAAGTAATTAACTTACATCCTTCTAAGTTACCACTACTTCGTGGTGGTGCACCATTACATTGAACCATTATTAATGGTTTTAAAAAATCTGCATTAAGTGTAATTCAATTGGTTAAAAAAATGGATGCAGGTCCGATTTGAAAACAACAAGATTTTTTAGTTAATAATGACTGAAATACTGGTGATTTATCCATATATGTAGAAGAACATTCACCCTCTTTTTTAATTGAATGTACTAAAGAAATTCTCAATAAAAAAGGGAAATGATTTGAACAAATAGGTGAACCTACTTTTGGATTAAACATAAGAAAAGAACAAGAACATCTTGATCTTAATCAGATTTACAAGAGTTTTTTAAACTGAGTAAAAGGTTTAGCTCCCAAACCTGGTGGTTGGTTAAGCTTTGAAGGAAAAAACATCAAAATTTTCAAAGCTAAATATGTTAGTAAAAGTAATTACAAACATCAATTAGGAGAGATAGTTAATATATCTCGAAAAGGAATTAATATTGCTTTAAAAAGCAATGAAATTATTTCAATTGAAAAAATTCAAATACCTGGAAAAAGGGTGATAGAAGTAAGTGAAATAATAAACGGAAAACATCCTTTTGTTGTTGGTAAATGTTTCAAATAGAATGATTACAAAGAGTTTTTTCCTTAAAAATTTTGATCGCTCTAAAGAATTAATTCCTCTTTCATATAACGATGTTTTTAGCGCTGTTAATCAGTTTCTGAAAAGCTATACTGACGTTAATGATATTGATATTATTGAAGAAAATTTGATTGAAGATCCAGTTTTTGAACTTGATGTTTTAGAACTTTTAAACGAGGATCCAATGCTATTATTGGACAGTAAAAATCCCCGTGTTCAAGAAGCTAAAATTATTGCTAATAAAGCAAAAAAAAATATTAAAGATTATTTTAATCTTCCCATTTTTTTTGATACAGATAGTCTAGATAAAAATGTTTCTGTTTATCAAAAAGCTGAGCTTACTGAAAAGAAAATTCAAAAAATAATAACATCAAAAAAATCAGCTATTATTTTTAAACCTATTTTTGAAATAGAAGATTGCTTAATTCAACCAGATGCTATTATTGTTCATGAAAAAGGACTTTGTGAATTTGTTGTTATAAAAGCTACGACCAATACAAAAAGAAAATATTTTTTGGAAATAATTTATGACTTTGTGTTATTTAAAAAAATAGGTAAGTATAAACTTTTAAACTATTATTTTTGTACTGTTAAATACGAATTACAAAATAAAAATAATGTTTCTTTCTTTTTAAATACTGAAATAAAAACATCAAAAAACAGTTTTAGTTTAAGTTCAAAAGAAAAAGATTATTTTAAGAATAAACCTTTTAATCACCCTGAAAAAATTGCTTACATACACAAAAAAAAGAGCAATGGAGTTAATGGTTTTTTGATTGTAAAACTCATTGATAACCTTATTAAAAACAATATAGTTGATTTAAACAAAATAAGTGATTTTGTTACTAAAGAAATTGACAGTAAAAGTGTTCGTAATATTCAACCACTTATTAAAAATGCGGCTAAAATACAAATTAATTTTTGAGATCAAATACAAGATATAAAAAAATATCAAGAACTCAAAATAAATCAAATTGTTTTTAATTACAGTGAAAATTTTGATTCTTTTTGGAGTAACTATTTATTAAGAAATTTAATTAAATTAGTTTTTGCTCATAAATACAATGAAATTTTTAAATTATCTGGTAAATTAGCTAATTGAAGTCAATTAACATATGCATATAAAGAAAATAAATCAATAACTATAAATCAACTGCTTCACGAATTAAATCAGAAAAAAAGCAAGGCTAATTTTAATAACTCAACTAATAAAATAAGTTTTTTTCTTGAAGCGTGAAATAGTGAAAAAGGTTTTGCAATTGGCAATAAATTTAAAAATACTTGAAATAAACTTAAAAAAAGAAAGTTTATTTTGATTTTGAAACAATTAGTTCATCAATCAGAATCATAAATAATTCATTACCATTTAGTCAAATTGTTACCCAATGCTCATTAATAGTTGATAAAAATGAAATAGATGATAAAAGAAAACTTAATTGTGAAAATCTAATTTTTGATCCCTTATTTATTAGCGTTAATGACTTTAAAAAAGTAATTGATTCACTCTATCAAAACAATTGCAGTGATTATAGTTTTGTTGTTTTTAATAAATCTTTTGAAAAAAACAGATTATTGGAAATGGCTACATTGATTAATGAACAAATATATAAAGAAAAAGTAAAAGCTATTGTTGACAATCTTTTTGACTTAGCTGATATTTTTACTATTGAAAATAACTGTTTGGCTTTCAAACAGCTAAACGGTTTTTCCTCTATCAAAAAGGTTTTAACTATTATAGATGAAAGCTTTTTAAAGGCAAGTAAATCAATTGGTTATCAAAATTTAAAAATCCAAAAAGGTGATGTAGCACAAGAAGTTGCTTTATCGCGTTTTCTAAATTGCTTAAATAAAAATGAATGAAATCAAGTAGCTTTTGAATTAAAAAAATATTGTGAAAATGATGTCAGAGCGATGATTTCAATTGTTTTATTTATCCAAGATTTAATCAAAAAAAATGATCTATTTACTTTTTATTCAGAAAATTAGAACCTGCTTTTCTACTATTTCACCTAATTTTTTTTAGGTTTAATAGCTAGTTTCTTACACATCTTTTGATTTTCTATCTTCTCTTTTAAATCACTAAAAAAATGGCTTCCTTTACTACTTTTTAAAAAACTTGTAAAAGAAGCCATTGCGTTGTGCTCTAAAACACTTTTATCATCGTAATTTTTGTTATTTGGGATAGCTTGTGATCAAATAATATCACCATCAAGTTCAATTATACTAAGTACCAAATTATTTGAAGTTAAGCTTACCCTAACTCTTGGTTCAGGTAATTTTTGCTCTTGAAAAAGTGAAAAGAAATCGTATTTTAAAATCTCAGAAAAAGTTCTTTCAAAGATATATTTTTCAACAAATTCAGTTGCTTTTTTCATCCCCATATCTTCATAAATGGCGCCAACCAAAGCTTCAAGTACATCACCAACAGTGTTTTCAGTTAGTTCAGCACCATTGCTTAACTTAACAAAATCACCTAATTTTAGCTCCATACCAATACGATTAAGATTTTCACCCTTAACAATTTCAATCTTAGTTCTTGTTAAAAGACCTTCGTTATATTTAGGATAAAGTTCAAATAGTTTTTTAGCAACAACAAAGTCAATTAAAGCATCACCTAAAAACTCTAAGCGATCGTAACTTTCACTAACATCTTCATGTTCATTGATGTAAGAAGCGTGAATAAAAGCTTTTTCAAAAAATTCTCAATTATTAGGAAAAATATCTAAATTCTTTAAAAAAGTTGCTAGTTTTTTATCAAAAATCTTATTATTTTTTAGTTTCAAAACTTTATTCTTCATAGTTATTCAAAGAAGTTTTAATTGCATTAATAAGATCAGATTTTAAGCTTAGATGTGCTAATCTAATCGTGCTTAAAAATTGTTGTTTATCAGCAGAACCGTGAGTTTTTAAAGCTAATTTATTTAAACCCATTACAACAGCTCCAGCGTTATTTTTGTAATCAAATTTTTTGGCTACACTTTTGATAATACCTAAGCTAAATAAGCCAGCAAGAGGATTTCTTTTATAACCCCTCTTAAGAATCCGTGCTATCGTTTTAAAAGTACCTTCCATTGCTTTTAAAACTAAATTGCCGCTATATCCATCAGCTATCAAAATATCACATATTCCATCCAGTAAAAACCTTGATTCTACAAATCCTAAAAAGTTAAGATTTTTATCAGCTTTTAAAAGCTTAAATGCTTCTTGATGATAATCAAAACCTTTATTTTCTTCTGTTCCTATATTCAATAGTCCAATTTTAGGAGTTTTTTTTGTAGTTGTTTTTTTAACAAAAATATCAGCCATTAAACCTAAAAAATAAAGTTCTTTTCCTGTGAAATATTTATTAGCTCCAACATCCAAAAAATAAAACCAATTATTGTTATCTGTCGGTACATAAGACATAAAAGCACTCTTAGTATTTTTATTAATTTTGCCAAAAGCATCATTTGTTAAAGAAGCATAAACTGCTGAAGAGCCTGCTGAAATTACAACATCAGCATTACCTTCACGAACTAAGTTTATGGCTATTTGCATTGAACTGTTAACTTTTCTTCTTGCACTTAGTGGAGTGTCGGTCATTTCAATGAAAGAATTAGCAAGTTTTTTTGTAATATTTTTTGGAAGTATATCAAGACCATCAAAAGCCTTTTCATCACCGATCAGAATGAAATTTAAGTCCTGATGAAAACTCCAATATTTCAATACTGCTTCAATTGCTTCACTAGGTTTGTTTTCAAAACCTAAACAATCAACTGCAAATCTAAAAGCCATTACTGTATGCCTATTATGTAAGAGAACACTTTTTGTTCTCCAGGTGAAAATTCACAAAACAATTTATATTTTTTTTCAACTAAAGCTTGCATTTTTTTCGCATTTTGTTCAGTGATGTCTTTGCCATAATAAGCCAAAAGGAACTCAGGTTTTTTCACTTTTTTTGAAAGGATATCAATTGTTTTAAAAAAACAGTCTGTAAGTTGAGATTCACTAGCAATAATACTTTTATTTGTAACAGCAATAAAATCATTTTTATTCACCATTACTTTGTTTTCTTTATATGACTTTGAAGCTTGTGTTATCGTAGCGGAAGCAAACTCTTTAATAAAGCGCTTCATTGCTTTCACATTAGTGTGAATAGAAAGATCACTGTTAAATACAGTTAAAGCAGCAATTGACTCCACAGGATTTGCTGTAATAACATAATCTGCATTAATCTTTTGGTGTTTTAACTGTTTAATTGTTTCATTGGCAGATAGAAAATAGTTTTTATCATGTAACAAAAAGATTACATTACTAGAATTGGTTAACTTAACAGCCTCAAGTAAAGAAAAAACAGAAGGAGCCCCAGTATCATCAGTACATAATATGGCGTTAATATCATGATCTTCACGAATTCTTTCAGCAAATGCTTCTGTAGGTACAGTAGCTACTATAGCAGGTTTTAAAACTCTTTTTGTTGGTGAGTTATTCTTGTTATTAACCTGTAAATTCATGTTATCAATTTTGACAAATTCAAATTCACCATAATTAAGACCCATTTCTAATAAAAGATGTGGTTTTAAAGTATGAGCATGAACTTTAACAAAACCATTGTCTTTATCACTAGCAATTACTACAGAATTGGCAATTCTATTTACTTTACTTTCAAACTTTTTTTGATGAAATTTTTGCTTTTCAACTGTCTGATTTATTTTTAAACCTAGTCTTAAAACATATTCTGTACAATAACCAAATTCATCTTCATTAGCATGTTGTTCTTTAAACTTGTTAAATTTAATTTCTATAGTTGATAAAGTATTGTCATCTAAATTAGATTCGCCCCCATAATATGACAACATTCCTTCAAGAAAACAAGCAAAACCATAAGCACCTGAATCAACCACACCTGATGCTTTTAATACAGGTAACATTTGGGGAGTTTTTTTCACTGCTTGTCAAGCTACTTTAACAGCTTGTTCAAAAAGTTTTTCAAGTGTTTTAGGACGATTTTTTTGACTCTTAAACTCATTGCTTATTAAACGAGCAACTGTTAGCATAGTTCCTTCTACTGGTTTTGATACGTTTTTATAAGCCACTTCTTGAGCAACAATGAATGCATTTGCAACATCTTCTATACTCAACTCTGAATTATTGCTTGTTTTATTTACGATTATGTTACTGAAAAATCCTTTCATTATTTGTGAAAAGATAACCCCTGAATTGCCACGTGAAAACAAAAGTAAATCTCTAGTAAAGTTTTTACCAAGTTCACTAAAGCTTTTTATATGACTTTTTTCATTTTCTAGTTTCTTGATGGCTTCTGTTATTGTTACTTTCATGTTTGTTCCAGTGTCACCATCAGGAACAGGAAAAACATTCAACTGGTTTATGTATTCATAATTTTTAGCGATGTTATTACAGCCAAGGCGTAACATATCGATAAAAGAAGATAAATTTACTGATGACATCTAAAGTAAAACCCTAGCTTATAAGGGATAAAAAAGAAGTTTATAACCACAATAATTTTAAATTTTAACTATTGAATTAAATATTCAAACCAAGGTACTATTTTACTCTTGAATTGCATTAATGAATAATTTGTTAAAATGGGAAACTTGCTCGTTTCTAGTTTAGGAAAAACTAATTCATAAGCGTGAAGTGCTTGATGCTTATATTTATTATTACGATTTTTAAATTGAATCAAGCTGTATTTTTTATCACCAACTAGTTGATTTGAAAATAAATTTAAACACGCACGAATTTGGTGAGTCCTACCACTAATTAATCTAATTGTTATTAAAGACATATCTTTTTGAGCTATGTATGAAGAATTTTCAAAAAAAGTGCTTATCTTTTTTGCTTCTGGAAAAGGTTTTCATTTTACTGTTACAATGCCATTATTGTTATCTTTTTTTCAATAAGCAGTTTGTAAGCCAAGATGGTTAAATTGACCAAAAACTAAGCCTTTATATCTTTTGGTTAAATGGTTATTTTTAAAAACTTTGTTCAATTCTTTTAAAGCTTTGTTTGTTTTAGCACCAATAACAATTCCAGAAGTATTACGATCAATACGATGTGCAAACTGGGGATAAAAGTTAAGTTTATTACTGTCAAATTGTCTATATCCACAGTGTTTTAAAAGCATATTTGATAAATTAATAATGCTATGTTTTTTATCAGGTTGGCAAACTATTCCAGTTGGTTTGTCAACTACAATAATATTTTCATCCTCAAATATAATTTTTAGCTTATCTTTTACCAAATCTAGAGATAAATAGTCATTATTAGTTTGTAAATAGGGATTTATATCAAAAAGAAAAGCTATCTCATCTTTAGTTTTTAATCTTGTGTTGACCTGTGGTTTTGTTTTATTTAACAAAACCTTTCCTAAGCGTAAATACTTAAAAAAGTCACTTCTTTTAATTAAGGGAAGGATTTTTAAACAAAATTGGTCAATTCGTTGGTTTTCACTTTCTTTAGGAACTAAAAATTTATTAGCTATTCTCATTTTGTTTGGCAAGAAAATCATCCATTATCTGTACTAATTTCATAAATTGATTTTTTTCATCCAAAACAAAAGCAGCTGCATATTTATGTCCACCACCATTGAACATTTGTGCAAAGTTATTAATTGGAATATTACGTGAGCGAATGGAACCAATTCATTTTTTAATGGACTCGTTAAAGTAAACAGTAGTCCATATTTTTACTCCTTTTATGTTATTCAAAGCATGAACCATTGGTAAAGGAGAGACAACACCAAAATGTTTATATGCTCCTTTTTTAATCAATGCATAAGCTAACCCATTTTTAGTTATTTTGGCTTTGCTAAGTACATAGCTAAAGTACTTATGTTCTAGTAATGGCTTTAAATAAACTGCATCATGAACCTTATTGCGGTTAAAACCAGTTTCCATCAATTTTGCTGTTAGGGCAAATGTTTGGGGTGTAGTAGTTGGACCTCAGAATCGCTGTGTATCAGTAATTATTCCTGCATAAAGATATGATGCAATCTCATCATTTAATTTGTAACCCATCTGCAAAATTAAATAACCAATCATCTCAGCAGTTGCTGAAAAAGAAGAATCAATTCATTCCATATCAGCAAATTTTTCAGTTCTAGGATGGTGATCTATCCTAACTGTCTCTTTTGCCAACTTATGTTTTTGAGTTAAAACTCTTTCTTGGTTGGAAGTATCAAAAATAATAGCTAAAGATTCTTTTACAAAATCATCATTTATGTCAGTTTGCTCAAAAGGAAAAAGTTCTCTACCGTCTGCATTAATATTGTATGAACCCATAACATAAGCTTTTTTTTCACTGAAAAAAGTATTTAGGAAAGTTTTGAAAGCAAAAGCAGATCCAAATGCATCAAAGTCTGGGTTTACATGTACAAATAAACTGAACTTATCAAACTGTTTAACCTTTTTTGAAAAATTCTTAATGAATTGTGGATCGATACTAATCATTGCTTAATTCACTTACTCCCTTTTCAATTAAATCATTTAAAAAAATAAGCTCACTTTCAAGCTTTTCAACATTTATCAATTTAGCTTTCGTTGTAGGATTTTTGGGAGAAAACTCACTACAAGTATCTAAATGTTGTTCAATAGAAATATCAAAAGTATTAAAAAATTTAGCTAATTCAATAATTTTATCTTTACTAAAGCCAATTAAGGGGCGAACTATAAATGTATCTGGTGTTGCTGATTGAATAACTTTTAAATTTTCAATGGTTTGGGATGCAACTTGTCCCAAAACCTCACCTGTAACTAGACAATCATACTTAAACATACTAGCAGCTTTATAAAAAACACGACGCATTAAAACGATACGATAAGATTCATTACTAATATGAATTAACTCTTTTTGAATTGCAGTGAAATCAAAAACTAATAATTTACCACTACAAATCGTTTTATTAAAAGAAATTAAATTTGCTAACCTTGTGATTTTTTCAATTGTTTTTTGATTCTTATTTGGTTCATTAATAAAGGTGATAAAATCAATGTTAAAACCACGTTGCATTACAAGTGAAGCTGCAACTGGGCTATCAATTCCTCCTGAAAGTAAGACTAAAGCTTTACCACTACTATAAACAGGTAATCCACCTTTACCTTTAAAACGTTCTGTAAAAACAAGAAAATGCTCCTTCAAAATCTCTATATTAGCTATTATTTCAGGGTTATTAATTACTCCTTTTAATTGGTATTTTTCAAATAGTTTAACTGCTAAATACTTCTTAAAATTACTTGAATTTTCAGCAAAATTTTTATCACGTCTCTTAACTTCTAATTTAAAGGAATTAAAATCTTTAAATAAATTAAAAAGAAGATCTAATAGTTTGTTTTCTTCCCTTACTATCTGTGATGCAAAAAAGAACAAACTAATTCCAGGCAAAAAACTGAATAATTCTTGGAGAATCGCTCTTTGTTCTTTTTTAATATCAAAGACAACAATTCTATCAAATTCATAAACAATACTATTGTTAATTTCCAGTTTTTTAAAAGCTTTTTTGATGTTAATTTTTAATTGTTTTGTGAAATAAGAACGGTTTTTTCCTTTTAAAACCAATTCACCATAACGCGCAACTAATACATCTTCACTATTCAATTCCATTTTCCTTTAATCATTGAATATCACTTTCTGTTAATTTAGGACTTTTACCAGTTAATATGTAATTTTCATATCAAATTCTCGCCTTATCAACTAAAACACTAAATCCCTTATGATCTATCTTGATAGGAACTAACCTAATTTCTCTATTGCTAGCATTAAATTGTTCTGGTAAGACATAATCTTCTGGTTTCAAAAAGGCAATTGCAAAAAGTCCTTTATTTACATTGCGCAAATATAGATAAAGACCAATTTGACAATAATATAAAGTACTGATAATAATTTTTCCATTACTGTCAAATCAACTATCTTTTTTGCCATCTTTCTTTTTAACAATAGGCATACCATTTTCATCAAGAATCATCTTGAGATTTCCATTGATTTTTTTATAAATAAGACTATCGCAAGAAGTTGTTTTAATCTCTAACATAGGAAGATCATTTTGATAAGCTAATTCACCATTTTCATCAAGAGGTTCACCATCAGGTATCCCACCAAAAACACTATCATCCTTAAATAAATCAAATTGAACTTTTTTAGGATCATAAGAATGAAAATTAAAACCAGTTTTTAAATTGACATAATCACGTATTTTAGGTTCTATTATGTTTCCAGCTTTTGCCAGTGTTTCATCAAATTCATCTTCATAAATTTTGACCATATTAGCTCAAGTTTTAAATGATGACTCATATTCAGATAAACCTAAAACCTTGCCAAAGCGAGTACCGGTTATTTTTTTAAATAGAGCTTTATTTCTTAAAAAATACTGTTCTGATAGAACAATTTGATTATCAACTATATCAAAATCAGTTTTATATTGCTTTATAAATCCCATTTATTTCATCAACTGTTCACAAATTCCTTTTAAAACAATTGATATTTCAGGATTATTAAATTCCTCATCATTATCTGGTTGTTTTTTGCTTAAAAATACAACACTTGCTAATTGATTAATGTTATTAATTGGAACTTCTGTCACATTTAAATAATGAACATCTTTTAAAGAGCAATTCTTGCTTTTAACATATGTTTCTAAAGCACTAAACTTGGAATTTTTTGTGCTAGTAAATAAAAAATTTTCGTTATAAAAATTGCTGTAATGAAACTTAGGCTGACCACTAGAAATATGTTCCATAAGTTGAAAGTCTAAATGATACTGAGGACTAAATACATAAATAGAACTAACATCCATTTCACTGAGATTTTTTAAGAAATTTTGGTACTTTAATGTTTGGTCAAATTCCGTTAATCAAATTAATTTGAATTGCTTATATTTTTCTAATGAAATAAAGTAATTAATTGTTGTATTAGGTTCCTCACTAATCTTGCTTTTTCCAATTGCAAAAACACCAGAATTAGTAATAATAGCATGGTGTACAATAGTGTCTATACTAACAGAATCTAAAAAAGATTGATAGATAAATTCATTGGTTTTGCTAATTCATATAATCGCGCCTGATTCTGCAATTATATCAACATCATCTAACGAGAGTAAATCAAGTAAATAAAGCACTGCTTGTTGTCAACAACTTGACATAAAAACAAGTTGATAGTTATTCGCTTGAATGAAATTAAAAAAGTTTTTTCATACTTCTAAATCCTTTGTAGTGTTAAAAAAATTTGCTGTATTAATATCTAAAACAACTATTTTTCTATCCATCTCTTCTAACTCTTTTAACAAAATTATTAAAGCTAATTCTGGTGATTTTTTCACCTTTAAAACCACGGCATGTCAACTTCTCTAAATTCTCTATTTCTTTATCTCCAACAATAAGTTGTAAAGGAATTTTTTCAATGATTGCTTGTCTAACTTTTTTAGCTAAGCGATCTTGATTATCATCTAAATTTACACGGATGTTTTCTTTTAGCAATTTGTTATAAAGTTTTTTTGCTGCCTTTAAATGCTTTTGGATATTAACAGGAATAATTACGGCTTGAACAGGTGCTAACCATAAAGGAAAATTACCACTTGTTTTTTCAAGTAAAGCAGCAATAAACCTTTCATAAGTTCCAATAATTCCAACATGGATAATAACTGGTTTTTTTAGTGTATTTTTTTTATCTATATAAGTTAGATCAAATTTTTCTGGTAGTAAAAAATCTAGTTGAATGGTGGCAATAGTAATCATTTTTTTAAAGATTGTTTTGAACTGAAAATCAATTTTTGGTCCATAAAAAGCAGCAGCTCCTATCTCTTTTTGATATTGGATATTTAAATCTTTTAAAACATTCTCCATTTGGCTTTCAGATTCTCTTCATAAACCAGGATTATCAATAAATTTTGATTGATTTTTAGGATCATGTAGAGAAAGATCTATCCTATCAAATATAAATCCAAATTTTTTATTAACTTTTTGAATTAAATTAAATGCGTTTTTAATCTCACTTTTAATTTGATCTGCACGACAAAAAATGTGATTATCAAGTAAAGTCATGCACCTCACTCTTTCTAATCCTATTAATCCTCCAGAGGCTTCAAAACGATGCAAAATAGAATCTTCTGAAAAGCGCTGTGGCATTTTTTTATATGAATATCGTTTTTGTTTGAAAATCAAACAGTGATGAGGACATGTCATAGGACGAAGCATCATTGCTTGACTATCAAGTTTAATAGCAGGAAACATATCTTCCTTATAGTGCTGATAGTGGCCGCTAATTTTAAAAAGCTCTATGTTAGCTAATACAGGAGAACAAACAGTATTAAAACCAAACAATAGTTGCTGGTGATGCACAAAATTACCGATTATATTCCTTAGTGTTGTACCCTTTGCTAATCAAATAGGAAGACCTGCACCGATCAGTGGGTCAAAGCTAAATAACTCTAATTGTTTACCTAAAGATCTGTGATCCTTCTTCAAGCGTTCTTCATTTTCTTTTATTAATTGTTCTAATTCTTTTTTTGATTGAGCAAAAATGCCATTAATCCTCTGAAGTTGTAATTGTGAAGGATCTCCCAAAAAATAATTTACGCTTACATTTAATAGCTTAATAAAACTTTTTTTAATAAAAGTTAAAGTTAAATCTTCTATTCAAAAATGTTTATTTTCAAAAAAGGTGATTTTAAATTTGTTTAAGTTGCTTTTTTTTAATAAGTTTTTTGTAAACTGATCATTTTCAAAAAAACTTAATGCTTCATCTAAAGAAACAAATTTTTGAGAAATCCCTTCTAGTTTACTAGAAAGAGAGTTTAAATCAGATTCTATTTTTGCAAACTGTTTTGTAGAAAAATTCTCATTTATATAAAAATCTAAATAAAATTCATCTCCATTAAAACCATGTTCACCAAACTGAACATTAGCATATTTGCATTTTAACCATATTTGTAATAAAAGAACTCCTGCAAGCATTAAGATTCTTGATAATCAATCATTACTTTTGAATTTGGTTTCATAATTTTGACAACTTCGATTGTTGTTTTTCAAATTCCACTGCGTTTTTCTAGGTAAGTGCGCAGAATACCTTCGATGCTAATTGTTTTAAATTTTTGAGTATATGCTTCAAGTTCAAATGCTAATTGACCATTTGCATAAAAAACAAAAAAATCAGTAAAACTTCGTTCACCAAACAAACGTTTCTGCTTAATAGTTACTAAAAATCCTGTCTTTTTGTGGCTTCACTTTGTGCTTTCTATTTCACCTTCCAAAAAAACGCAATTTAACATGCTTTTTAGCTAGGTAATTGACCTGTTAAGTTGAAATGAAATTTAAAGTGGTTAATTGTTTCTAAAACCATTCTTTCTTCTATTAATGCAGTTCTAATACCTTCAAACTTTTGTTTATCATCAAGATATTCTCTAACATTCTGATTAGTTTTTTCATAATACAAGCTAATAACTCTTTTAACAATATCATCACTAACTTCAACCTTTCATTCCTTTTGTAAGAAATTAAATACTAAAGCTTTTTTAATGATTTTTTCAGCTATTGATTGAATTGTTTTTTCATCAGCATCTTTAACAACATCTCTTTTCAAGCCTTCAACAACATTTTTTAACTCTTCAGGGTTAATTTGAAATTCAAAATAACTAACAATTTCATTCATTGCTGCTGAAAACAAATTATCTTTAATAACAATATGAGTTAGTTCGTGGTAAAGAGCACTTTCCTTAGCATCTTTAAAAGAAGCTAAAATTTTTTGCCGATGTTGCTCAATAAAAGCAGGATCTGCAAAAATACGATCAACTTCAATTACCTTATCATACTGAATAGATTTTTGCAACTTTGCAATAGATTTAAGATTAGTAGCCATATTTTTAGAAACTTATTAAATTTTAATTCCTATATGCGTGAAGAATTTACATTTGTTGTGGTGTTGAAATCTTCAAAACATCAAGGATTTGTTTAACTATAAATTGTAATGATCTCAATAAAGCAAGTCTTTGTGCTGATAAAAGTGAATTTTTATCATCATTAATTTTGCAAACTTTATACCAGGAATGAAATAAACTAGCAGTTTCATATAAAAAGTTAGTTAATAAGTGCAATTCGCCTGTTTCCATAGCTTTTTGCAACATAAAAGGATAATAGACAAGTTGATTTAAAAGTGATATCTCTTTTTCATTAATCAAAAGACTGCAATCAGTAATTTCTTTTAATTGATCAGAATTTGCAATTCTAAGAATACTATTCATTCTTGCAAACGCATATTGAACATAATAAACTGGATTAGCAGAGTTTTGCAAATTAGCTATATCTAAATCAATTTTGATAATTGTGCCATTATTTTGAGATAACATAAATCATCTAGCAGCATCTTCACTAATCATTGAAAGCATTGTTTCAATTGTGATTACATTTCCAGCACGTTTTGACAAACGTACTAGCTCTTTATTTTTATAGAGAGTAACCAGTTGTAATGCAAAAATTTTTAAAGAAGTTTTAGTGGTATTTTTTAAAGCATCAAATGCACAATACATCCTTTTAATATGTCCTTCATGATCAGTGCCTCAAACATTAAATAGAATGTCAAAGCCACGTTGTAATTTTTCTAAGTGATAAGCAACATCAGTTCCAAAATAAGAAGCTCTTTTATCACTTCTAATTAAAACTCTATCCTTATCATCTCCATAAAGCGTAGTTTTTAGTCAGAGTGCATTATCTTGATAAAAATAACTATTTGGTTTAAGTTGTTCTAAAAGATCATTAAAATTTGTTTTTTTAACAATTTCGCTTTCAAATTTTCAACTATCAATAAAAACATGGATTTTATTCAAATCACTTTTAATTAAAGACAAAACTTTTTGGGTGCAATTAACTAAAAAATGATCTAAGAATGATGTTTTACAAAACTCTTCAAAATTTAAATTATCAACTGGAAAATTTTCAATTTCACTAGCAATTTTATCTATTACTATTCCACTATATCCATCAGGATGTTGCTGGATAGCAATATTTTTTTTTAACTGCAAACTTTGATAAACACTAAAGCTAAAAACTCGTGCTTGTTGTCCATAATCATTTACCCAATATTCACAGACTGTTGTATAACCCAACAGCTTGGCTAAATTGTTTAAAACATCACCAAAAAAAGCTATTCTCACATGACCTAAATGGATCCTTCCGGTAGGATTTGCTGATACAGATTCAATTAAAATCTTTATATTTTTCTTAGTAAAGCAAGGTGTTTTTATCAAGTATTCTAAGTACTTTTGATAGCTAATTTGAAAGTTTATAAAATTATTGTTAGCAATAAAAACATTTTGGTAGTTATCCTTTTTATTTTTTAAAAATCACTCAACAATACTATCTGCAATTTTTTTATGATTTTCACTTTTTAGTTGGAAAATAATAGTTGAAGAAAAACCATTAAAACTATTATTTTTAACTAGTTTAACAAGTTCCTTTTGGTCATCAAATTTAAGCTTTAAAGCGCTAATGCATTCTTTTAAATCATTGATGATAAAAAACATTACTTTAAATTATATTTAATGATGCAAAATGTCTTTTATCATAACAGTAATAGGTGCTGGGCATGCTGGATTGGAAGCCGCTTTCATTGTAAGCAAATTCAACATCAAAGTAAACCTTTTAGTTCTTGATATAAATCATTTAGGTTCTTGTCCATGTAATCCTTCAATTGGTGGACCTGCTAAGGGAATTGTTACTAGGGAAATTGATGTTTTAGGAGGTATGCAAGCAATTGCTGCTGATAACAATGCCTTACAATATAAATTACTAAATAGTTCAAAAGGACCTGCTGTGCAAGCTATCAGAGCACAAATTGACAAAATAGGTTATAAAAACTGGTTTCAAAGTCAAGTTAAATTAAATAAAAACATTAATCTAATTCAATCTGAAGCAATCAATTTAATTGTTAGAAATGAAAAAATAAAAGGCGTTATTTTAAAAGACGGAAGTGAACTTTTAAGTGATGCGGTTATTATCACTACCGGAACGTACCTAAGATCAAAAACATACTGTGGTAATACAGTTAAAAATCAAGGACCTGATCAATCTAAAAATAGTGAAAAATTAAGCACAAACTTAATTAACAGAGGTTTTAAAACAATTCGTTTAAAAACAGGAACTCCGCCAAGAATTTTAAAAACTTCACTTGACTATAATCAAATGGAATTAGAAATTAATAATAATCAAAACCTTGCTTTTAGTACTACAAATAAAAATTTCTTACCACTTGAAAAACAAATACCTTGTTACTTAGTTCATACCAATCAAAAAATTCACGATCTAATCCTTAAAAACTTAAAAAAATCTGCAATGTTTAATGGTAGTATTTCAGCACAAGGACCACTTTATTGTCCAAGCATTGAAGACAAAGTTTTTAAGTTCTCTCAAAAACCTCGTCACCAAATTTTTGTAGAACCTGAATCATTGAGTCTAGATACTATTTATTTAGCAGGATTATCAACTTCTTTTACACCAGAAATTCAAAAAGAAATCATCCAGCTTTTACCTGGTTTTCAAAATGCAGAAATTAAAAAGTTTGGTTACGCTATTGAATATGATGCTTTTCTATCTAATCAACTAAAACCAACACTTGAAACGAAGTTAATAGAAAACTTGTATTTTGCTGGACAAATTAATGGCACTAGCGGTTATGAAGAAGCTGCTGGTCAAGGTTTGATGGCAGGAATTAATGCTGCTTTAAAATTATTAAAAAAACCACCATTTATTTTGCAACGTAATGAGGCTTATATTGGGGTTATGATTAATGATTTAGTTACTAAAACAATCAGTGATCCATACCGTTTGTTAACATCCAGAGCAGAATATAGACTATGATTGAGAAATGACAATGTTCAAGAACGGCTCATTAAAAAAAGCTTTGAACTTGGTTTAACAGATAAAAAAACATATGAATTGTTCCTTAAAAAGGAAAAGAAAAAACAGGAATTAATTTCATTTTTAAAAAACACTCAAGTAGGCAAGGTTAAAGCATTGAAATTCACTAATAAAAATACCGCTCAATCACTTTATGACTTCAACAAACGAAGTGAAATAAATTTAGATAAATTGATCAAAGATCTTCCTGAAAAATACCAATTAGATTCAGAAACACTTAAACAAATTGAAATTGAAATTAAATATGAGGGTTACATAAAGAAAAATGAAAAGTATTTTAAGGGTTTAGATAAATTAAGCAAAATTAAAATTCCTCATACTTTTGATTACCATAAGGTTAAGAATTTAGCTAGTGAAGCTATTTTTAAACTATCTAACTTTAAGCCTAGTAATTTAGCAATTGCAAGTCAAATAGCTGGAGTGAACTTTAATGACATTATAGCCATAAAACATTTTTTAAAAACTTATGAATAATGCTAATTTTGAAAAATATGTTGATTTAGTTTTTGAAGCAAACAAAAATTTCAACTTAACAGGATTTAAAACAAAAGAAGCTATTTATCAGAATTTAGTTATAGAAATATTGACATTATTTAAAGGATATGAAAAATTTTTTATTGACAAAACTGTAGCAGACTTGGGAAGTGGAAATGGTTCGCCTGGGATAATATTAAAACTGTTATTTCAAAAAATAAAAAAGTTAGTTTTAATTGATAGTAAACACAAAAAAATTAGCTTTTTAAATAAATTAACTAAGCAACTAAATCTGGAGAAAACTGTTGCAATTTGTGAACGAATTGAAGTACATAAAAATCACTATGATGTTATCTGTTCTCGTGGTCTAAGTACGATTATTAAAGTTAATGATTTAGCATTTTCCTTGCTTAACTCAAAAGGTATTATTTTTCATATAAAACAAAGCTTAGACCAATACATTGAATTTGAAAAATCAAATCAGAAGAATCAATTTAACTTGTTATTTATAAAGCACTTTACTAGTCAGAATAAAAAACTAATTTTGATAGCTTTACAAAAAAATGATTAAGTTAGGTTGAGCGTCTTTTAGTAATACAGAATTTGCTCAAGATATCTTTATAAAGTTTGCCAATCAATTCTATAAACAAGATGATGCTGGAACAATTTTATTTGAACTAAAAAAAACATTAGGAGTTAATCAACTAGATGAAATTGAAAAAAATAAAAAAGTTATTATTGTTAATCAGTTTCAAAATAGCTTAGGCAAATTTTTACTTTTTAATAAAGAAAATACTAAAAGAATAAACAGTTTAGCTAATGAACATATAAGTTCTTTTTTAAAAAAAATTTTCAGATTATCTGGATTTAAAGATATGTTGATTGATGTTCAACATCATAAAAAGTTACAAAAATGTTTATTAAGAGAAATTCATCTTTTAGTTTGTTTAATTAATAGCAATCAATTTAGTGATGAATTAATGCAAATTATTGAGTGGTATCAGTATTTAAAAAAACACTCTAGTAAACTTTTTGTTATTACTGCAAGTAGTGATAAAAAACCAGTAATAGAGCCAACTATAAATGAATATAAAGCTATTTTTGGAGAGTATTTATCTTCATTTCACCTAGATCTAAAAAATAATCAAAGTAATGATTTATTCCAGAAATTACTTGATCAGATCAAAATAAAAGCTACCTCAAAAACTAGTTTGAGATAGCTAAAAATATAAATGGCGCATCGGGAGGGATTTGAACCCACGACAACACGCTTAGAAGGCGTGTACTCTATCCACTGAGCTACCGACGCATTTCTTTACTAGAAACTATCATATGAATCTTCAATAAGACTCTAATAAAAGTTTAGATGATTTTTAACTTTTTTATTGTATTCATGCAATAAAAATTAATTTAAATAAAAGAAATTTATTAAAAAATCCTTAAAGCACAAATATCAATAATTTCCTTTTCTATTGATTCTTAACTATTTTTAAAAAAATAGTCAATTTATTTAGTCACAAAAACCTTAGTTAAAAATAAATTTAAGTTGCAATTGGATTGAACATATTATTTAAACTTTCTTTTAAATTAAGTGTTTTAAAAGTTGATTTTTAATAAATTAATTAACAAAATAAGATAAATAGTTAAAAACTCAATAATAAACGCTTTAAAATATTTCACTTTGATGGATGAAAAAGGGATTTTAGTTGCAATTAGTGGTGGTAGTTGCTCAGGAAAAACTACTGTTGCTGAAATGATTTATCAACTTTTAAGTAAAAAATTAAAAGTTGCGATCATCTGTCAAGATAACTATTACAAGTCCTATAAAAATAAGCCATTATTAAAAAGAAAAACAATAAACTTTGATCATCCTGATGCTTTTGATTGAAAACTTTTAAGATCACACATTGAAGATCTTCTAAACGGTAGTATAGTTAATGTTCCTTTATATGACTACATTAACTATACCAGAGCTAAAAAAACAGCAAAAATTGGTCCAATTGATGTTGTTATTCTAGAGGGTTTAATGCCATGATTTGATGAAAAATTATCAAGACTTTCTAAGCTAAAAATATTTATAGAAACAAATGGGGAAGAACGTTTAATTAGAAGAATAGAAAGAGACTGACAAAGGGGAAGAAATATTGATTCTATTATTAAACAGTGACGCGAAATAGTAGCACCAATGTATGAAATATTTGTAGAAAAAATGAAGCGAAATGCTGATTTAATTCTGCCTTGAAGTCAACGCAGAGAAGTAAGTACAAGTGTATTGGATGTCGCAATTGAACACTTATTTCACAAAACTGTTGAAAAAAATAATTAGTTCTGGAAATTATAGAGATGTTTAAATTTCAAAGGTGAATTATGTTTATGCTCTGCTTTTGCTTTCAAATCTAAAACAATTTTTTGCTTTTCTGAACTAAGTATTAAATCACCTTTTAAATATTGATCAAGTTCTTTATAAGTAATGCCCATCTCTGTCTCATCAGTTTGCCCTTCAAAAAGACTAGCAGTTGGCGCTCTTGTGATTACAATTTCAGGAATATTAAAATGCTTAGCTAATTTGTAAACGTCCTCTTTTAAAAGCCATGCTAAAGGAGCAATATCACAAGCTCCATCTCCTCATTTTGTGAAATAACCAAGTGTATACTCTACAAAATTACCAGTACCTAAAACTAAGAAGTTGTGTTTTTGAGCATAAGCATATAAAGTTATCATCCGTAAACGTGCTTTAATGTTACCAGCTGTTAAAAAATCTTTTTTTGGATCTATTCCAAGGGTTTTTACTAACAGATTGAAACTCTCTTCCAGTTCAATGTTAATACTATTAAATTGCATTTTTTTAACAAGTTCACTAGTTGCTTGAAAATCAAGTTTTGAATTATTAATATGCATTATTAAAGCTAAATGGTTTTCAAAACCAAAAGTTTCTTTAGCAATAGCAGCAACAACTGCTGAATCAATTCCTCCAGATAAGCCAAAAATAACACCTTTAGCTTTAGATTTTTTTACATAATCAAACAGCCAGTTTTGGAGTTCTTTTAAATATTTAATTAAATTAGTCATTAATTACAAATTCACAACCATCAAAGAAAATAATATCACCTTTTTTAGCACCAACGGATTTTAATTGATCTTCAATACCGATTTCTTTAATTTTATTTCCTAAGCGACGAATGTTATCAAGCGTAGTTTGTGGAATTTTATCAAATCAATATCTTAGACGTTTACAATTAACAATTCATCTGTTTAAACTATCTTTTTCAATATTTAAAGGGTCGTTATTATTTTCATTGGTATTTTGAAAAACATAATGCTTCTCAAGTTCCATTGGCAAACTAAACTTATTAGCTCCAAATTGGGAAATTGTTTTATTGTAAAGTTCAAAAACTCTATCAAGTAAATTGCCTAATTCTTTCTTTAAAGCAGAAATTTTTAATACAGAAATAGATTTTTTTTGAAGAAATTTTTCTAATTTTTTAAACCGTTTCTCACCTTCATTAACATCAATTTTATTAGCTACAACAAGCATCTTTTTTTTAACTAGAAGGGGAGAATATTTTGAAAGTTCATCCATTATTTGTAAATATGCTTTGCAAGGATCATCATTATCTACAGGATCAAGTGAAATTAAATGAATTAATATTTCACACCTCTCTATATGCCGTAAAAAATCATGTCCTAACCCACTTCCTTCACTAGCATTTTCAATCAAACCAGGAATATCAGCAAAAACTAAACTATTATTTTGATATTTAACTACACCAAGTACGGGTATTAAAGTAGTAAAGCGATAGTTTGCAATTTTAGGTTTGGCATTTGAAAGTTTGGAGATTAGTGTTGATTTACCTGAATTTGGAAAACCAACAATTCCTACATTAGCTAAATATTTAACTTCCAAACTAACGTTTAAAATCTCTCCTTTATCACCATTTTCATATAGATTAGGAGCACGCATAATTGGGCTTTTAAAAGCAGCATTTCCTTTTCCGCCTTTTCCGCCAAAACACAAAATAAAACTCTGTTTATCATGAACAAAATCCACCAATACACTATTATTTTCTAAGTTTTTAACTGTTGTTCCTATTGGCACTTTAATTAAAAGATCTGAACCATTTTTACCATGTGCTAAATCTGGTTTACCATTTTGTCCATCTTCTGCAAACAGGTGTTTTTTATTCTTCAAGAAAAAAAGTGAATCGCAGTTATGATCAGCTTGCAAAATAACATTACCACCATTACCACCATTACCACCACCAGGACCACCTTTATCATAATGTGCTTCTCTTTTTCATGCTATGATTCCATTACCACCATTTCCAGCAGTAAAGCGACACTCACAATAATCTGTAATAGCCATTATTTTGGTTTCTTTTTCTTTATTTTAAAAACGATTTCTGCAGATTTTTTTCTTTCTAGATCACTTTGAATTTGTTGCAACTTTTGTCGATTTTTAGTTTTAGATTTACCTGTCTTTAAGAGATTTTCAGCAATTATTATTGCATATTTTGATCTGATTAAAAAAAAGTTAGTCATTTTATTCAAAAGAAAAAAACTTAGCATTAATGTTATTAGAACTAAAAAAAATCGAAGTAAGATAATTCAACTATTTCAAGCCATTGGTATTATTCCTGATAAAAAAAGAACTATTAAAGTAAAAGCAGTAGAATAGCAAAAAGGAATTAATTCCCAAATAATGTATTTAGTAAAAGCAAATAAAAAAAAGGCTAAAAAAAGATAAATTAGTAAAGGAACAAATAAAAATCACAACAAAACTAAGTCAAAAATGGGCTCTATTTCCTTACCCATAAATGTTCTGTTTTGTGCTGCAATTAAAAAATTAAAGCCTAGTAAATTGATTTCACCAAAAAGAATTCAAATCAAAAGATAATAAATAAAAACTAAAAAGAAACTAAAACTTCCTATTAGTCAATTAGGGGTTTTAGTTTTTTTATTTGACTTGAAAAAATAATTCAAAACTAATAAATTTGTGCAGTAATTTGAAATTTCACAAAGCGATCGTTTTCATAAAATTGATAACGAAGAAGACCCTTGATAACTCAAAGAATAACTGGTAATCCCAGGTTTTGAATTTCTTTGCAGTTCTTATGATAAAAACTCTCGTTTGGCACTAAAAAACGGCAATATCTCATTAATTCGTCTCTAGAAGCTACTAATAAAGCTTTTTTGTTTGCAATTACAAAACCGGTTTTAAAGCCTTTGAAAGTCTCATTTATTAACTTAATGATATGAAAATTACTACTTAACAAAATCATTTTTTCTTTAGCTAAGACAAAATCTTTGCAAAGCAAATTCAACTGTTCTAATAATGAATTGTTATTTTCTTTATCATCTTTAATATGAATCAGCAAATATTTAAAGGGCTGAATAAATTGCGTTAAAACTTCCTTTAAAGTTTGAATTTGTTGTGAAGTTACCTTAAGCTTAAATTCACTTGCAAGGTTAACCTTTTTAAGATCAACTAAATTAATTTGGTTTAAATTCAAGTTTTTATTAGAAACTTTAAAGTTTTCTTTATGAGTTACTACTATTTGTTTATCTTTGGTTAATTGAATATCAATTCAAATACCATCAAAATCAAAAACTTGGGCTGCTTGAAATGCTAATTTGGTGTTTTCTGGTGCAATAGAACTATAACCGCGATAAGCTATTAAAAACTGTTTTCGCATTACTATTTTTTATCATTACTGCCAAAAACATCCTTAGCATCATCTGCTGAAAATTGACTAAGTCATTCATCTGCGTCAATAGAGTCAATATCATAACCTTCAAGAGCAGGAATATCCTTAGTTATATCTTCATTTAAATTCTCAGTTTCCTCAGACTGGAATACTAATCAGTTACCTTCACTATCAAATTCCCCATCATATCAGTTTCATTCATTGTCTTCATAGTAACCATATTCTTCATTTCCGATTAATTTCTCTCAAAACTTAGGTGCCTCGTTAATTCGTGGTTGACGATTCTTTCATTGTTCATCAACAAGAACTCAGTCTCCAGATTCATCATAGAAACCATATCAAATCCACTCACCTTTATTGTTGTACCTACCATAAACTTCATCACCAATAAGTGGTAAATAATCTCTTTCTCTAGCTCATTTAACAGTTGCATCCTTATTTCATTTTTGATCATCACCAAAGAATCCTGCTCAAACTCATTCTCCATCAGGTTCATAATGACCATAACTATTGTTACCAACTAATAATTCTCAATCAAGTGATTCTTCTTCACTAGCTTCTGGTTGAATTTCATCAGCTTGAGGTTCTTCCAATACTGTTTCAGGTTTTCGTTCTTCAAAGTTAGGTTGAACAACTACTTCAGGTTCACTAGAAACTTGAGGTTCTTGAATAGTTTCAACTGGTGAATCAAATTTAGCTTCTTGAGGAGTTTGTTCAGGTTGAACAGTATCAAAGGTAGCTTCTGGTTGAGATTCTACAGGTTGAATTTCTGGTTGAGTTTGAACTTCTTCAAGGAGAGGTTCAGGTTGTGAATCTTGAACCGATTCAACTGGTGAATCAAATTTAGCTTCTGCAGATGGTTCAAAAACTGCTTCTCCAGGTTGTTGTTCTACTTGAGGTTCAAATTGTGGCTCTGATGGTTTTGATTCAATTTCATAATTTGGTTCATCAAAGTCATAATTAGGTTCATCTACATCATATTGAGGTTCTGATGGTTGTAGATCTGAATCATAACTAGGTTGATCAAAATCATAGTTAGGTTCATCAAAGTCATAATTAGGTTCATCAAAACTAGCTTCTGATTCAACATGTTGTTGAGTTGGTTCACTACTAAATTCAGGTTGTTCAACAGTTTCAACTGGTGAATCAAATTTAGCTTCTTGAGGAGTTTGTTCAGGTTGAACAGTATCAAAGGTAGCTTCTGGTTGAGATTCTACAGGTTGAATTTCTGGTTGAGTTTGAACTTCTTCAAGGACAGGTTCAGGTTGTGAATCTTGAACCGATTCAACTGGTGAATCAAATTTAGCTTCTGCAGATGGTTCAAAAACTGCTTCTCCAGGTTGTTGTTCTACTTGAGGTTCAGAAATAACTTTTTGTTGATTTTCAACATCATCAAACTTAACTTCAGTAGTTTTTTCAGGTTGAACATCAAAAACTGCTTCAGGTTCTCCAACAGTATCAGTAGTTGTTTGATCAACTACTTCAGCTTCACTAGAAACTTGAGGTTCTTCCAATCCCTCAGTTTGAGTTTGATTTTTATCAAAAACTGCTTCATGTTTAACAGTATCAAAGCTAGCTTCAGTTGTTAATTGATCAACTGGTTCAGATTTAGGTTCCTCTTCTAATATTTTTTCAGTCTGTGTTTCTAATTGAGGTTTTTCAAAAACTGATTCTGATGCAGCTTCAGATACATTATCAAAAGTAGCTTCAGATTTTAATTCTTCTGTTGATAATGGTTCAGAGGAGGTTTCTTGTTGAAGTTCTTTAGTTTCATCAAAAGTAATTTCAGCTTCTGTTTCTTTAGGTTGAACTTCATTATTTTCAACATTATCAAAGCTAGCTTCCTCTAGTTTTAAATCTTCTAACTTAGTTCCATCAAAGATTTGTTCAACTTCAGCAGTAACAATTTCTGGATGATTTTGATCTAATGCAGGTTCAGATGGTTGATCTTCAGAGCTAGGTTCTAATTGGGTTTCAATTGTTTCTGGTTCAAAAGCAGTAACTTCAGTAGGTGTAACAACTGTTTCTTCAGACTGATTTTCTAATTGTTGATGATCAAAAACTGCTTCAGTTTTTTCTTCTAATTTAATTTCATCAGAGATTTGTTCAGCATGAAATTCAGCTTGCGATGATTCTTGGGGAATTTCGACTGGTGAATCAAATTTAGCTTCTTGAGGAGTTTGTTCAGGTTGAACATCTGGTTGAAAATGATCATCAAAAACAACTTCAGTAGTAGCTTCAGGCTGTAGTTCTTGAGTTTTAATTTCATCAAAAACTGCTTCTGGTTGAACAGTATCAAAAGTAGCTTCAACTTGTGATGATTCTTGAGGAACTTCAACAGTTGAATCTAATTTAATTTCTTTGAAAGTAGGTTGGAATTCTGATTCAGAACTCTCTTTTTCGTCTGGTTTTGAATCAAATAAAGCATTTGATTCAATGATTTCAGTTAAATCAAGCGGTAGAGGAGTGAATAAGTTATTTTCATCTTGATGTTCAAATAATTTTGTTTCTTGTTCAACTGTGGGTACATTTGATTCAGAAGTCTCAACCTCTTGAGTTTGACTAATGAATTCAAGCGTATCTTTTTGTTCAGATGAAAATTCATTAGGAGTTTCAGCACTTTCAGTGAAACTGTCTTCAACTATTTGATATGTTGGTAATACGAACTCACTATCAGTTTCTAGTTGTGTTGTTGGTTGTTCTTGAACTTCTTTTTCAGAAACATCTAAATTAATAAATTCATTACCAATTTCTTCATGATTTAATTCAACAGATGTAGTTTTAGTTTCTAATGATTTATCAACATCATTTACAGAATCTAATTCAAGTGATAGGTCAGGTTGAATGAAAGGTGGTTCACTTTCTACAACTATTGTTGTTTTTTCATCAAATAATGATTTTGGTTCAGTTTCAAGGAAATCTACTTTATATTCCAATCCTTCTTGAGGTTTTGGATCATTATTAATAAAAGTTATTTCATCAACTAGTTCACTTTTGGGAAGTTCACTTGAAACCTTAACTTCTGTTTCTGTTGAAAAAGGAGCATTAACATCAACATATTCATTTGTTAAGAGTACCTCATTAACAAGTTTTTCACTAAATAAAGCAGTTAAATCAGTATTAACACTTTCATCACTGTTTGTTTCTTTTATCTTTTCATCTGAAACAACAGGTTCGCTAAATTCTTGTTGAAATTCATTAACAGAATAAGTAGGTTCAAATTGTTGTTCTGAAACTGTTTCATTTATTTGAGAATCAATATTATTAACAACTTCATCTCTAAAAAGGGATTCATTAATGAAATCATTATTAGCATCTAAAATAATTTCATCTACTTGTGATTTTTCTTCTACACTTTTTTGTGTTTCATCAGTAAAAGAACCGAATTCTTTTCAAACTGAATCAATATCATCAGAAATATTAGATAAATCTGCTTTAGCACTATCTTTTATTTCTTTAGAATAATCTTGACTTAAATGTTCAACATGTTTAGCAATTTCATCATGTTGTTGGTCGTCTTTAATAATAGAATCTAAGCCTTTAAGATCATCTTCGTAAACAAAAACGGTCTCAACTTCTGGAACATTTCCTGCTTGAGTTTGATTGGAATCTAAGCCCTTAAGATCATCTTCGTAAACAAAAACAGTCTCAACTTCTGGAATAGTAGTTTCAGGTTCACAAGCTGCTTCAGGTTCAATTTCAACAGGAGCCTCAACTTCAGATTCAACCTTAACTTCCTCTTTAACGATTTCAATTAAAGGGATGGTTTCATTAGTTTCTTTAACTGGATTTAAGAATTCAAATTTTTCTTTTTCAAAATGTAATTTAGTTACTGGATTACGGAAGATAACTTCATTTCTTCTTGGTTTAACTTCTTCAATTACCGGCTGCTCTTCACTTGAATTTTCAAGTAATAGTTCGTTTTCTAGAGAAGAAGCTAAGCTCTTGGTATTAAATACTGTAACAACAATGTCATTTTCCTGTTCTCAGTATGGTTGTTCAGAAATAGTCTTTTTCTCTATTTCATCAACTAAGCTATCAGTATGACCAAGTGCTTCTTCAGTAAAGCGAACAGGATCAGGGATCCAATTTAGTTTCTTGTCAAAAAATCCTTTTCAGATCCATGTATTATTTTGAACAAAACCATAGTTAGGGTTGCCTACAAGTGGTCTTCAAAAATCAATATCAGCTGGTCTTTGTTTAATGAAAGCTGCATGTTCTTGAGGAACTTCAACTGTTTCAATTACCTTAAAGTAGTTTCATCTTTGTAATTGGTCAAAATAACCTAGTCAAACTCAAGTATGATTTTTTCAAAAACCATATTTTCTATCACCAACAAAGATATATCAGAACGCTTGTGAATCTTCTGGATTTAATTTTTTTTCTCTAATAGTTGTTGTAAAACTATCTTTTTTTGTTTCTAGAACTTTGGTTTCTGGTTGATTGTTTAATTCAACCAACTTATTTTCATTAATGGTTGGATAACTGCTTGTTTGATTTGGAACTGTATTAACAACAGTTTTTTGATTAAGTTCTCTTCTACTTAAGATATCTGTTTCAGGTGGAACAATAAAAACACCGCGCTTGGTTTCATCAAAAAAATCTATAGGTGATTTGCTCTTTGATTTAACTGTTTCCTTTTTCTTACTATTAAAAGACTTTGTTTTCTTGAGAATGTTTTTTTTGATTTCACCTAAAAAAGGTTGATCAAAAAGATTTTCAACTTTATCTTGTTCTTTTTTTGGTTTAGTAACACTCTTCTTGTTAGCTGATTCTAAATACTTTTTGGTTTTTGATTTAGGGGTGGTGTTTTTGTTTTTATTCTTTGTTGTTTTTGGCATTAATAAAAATACTTAGCACTAGTGACTAAAGTTATCTGACTCACTAAATTGTAATTTTTAATATCAAGATAACAAAAGTTAGCATACATGCTAAAAAATAAATAGATTTTAATTACTATTTATTTAATTTATTTAATGTAGATTTAATAGTGTTTCAGCTTCTTTTTGCAAAAGAAAAATAGTTATAAAATCAAAAATTAATCACTTTAATTTCAAAAAAATTTGACAGCTAAAGCTTTACATTTTATTAACAACAGAACAAATAATTTTTAGCTTTTAAAAATTTATTTAGAAAGAAAACTATAAACAGGCAATTTTTGTTTTTCTGTTTTTACAAAAATATCAATAAAAATATCACAGTCAAAATGATTTAATAGTTTTTTGCGAGTTGCTATCCCAATTGCTTTGATCATTTCTGCATTTTTTCCAATGATAATCTTTTTTTGGCTTAATTTTGGAACACTAATTACTAGGTGAATTTTTAACAAGTTTTTTTCTTTATTGAAACTCTTTTCAATAATTTCAATTCTTGCTATATGGGGAATTTCGTTTTTGCAATAAAAAATTATTTGTTCACGTAATCCTTCAAGAATCTTAAAGTCATCGTTGTTTGCATCAATAAAATTGATATCTTTACGAAAGATATTATACCTAAGTTCAACTTGTTTAAAAATTGATCAAAAGAGATTTTTATCAAACTTAAGTAAGTTTATTTCAATTGTTTTTTGTGGTTTAAACTCTTCTAAAATAATTGCTTTATTAACTTCACTTAAACTTTTTTGATGAAATTTATTAATTAAAAAAATTCTTGTTAAATTTTGATAGCGCTTTAATTGTTGTAATTGAGTTTTTAAAAATTCTATCTTGTTATTCTGATCACTTCTAACAACTAAAAGCAATACATCAATTCCACTTAATGCTTTGCGAATTTCTTTAGTTATTAATTCATAATTTGAATGTTTTTTCTCAGTAAAACCAGGGACATCAATAAAAACAATATTTTTATTAGCTTGGTTAATTACCTCAGTGGAAATAGAAAGAAGCGTTGTGTTATTCATGGATGATACCATCAGACTATCATCGTTATGTAAAAAATTAATTAGCGTTGATTTTCCTGCATTAGTAGGTCCTAAAACACCAACTTTTAAAACTTTCATACTGATAAGATCTCTTCATGAATTGCATCTTGAAAAAACATAGTAACGCTTTCAAATGCTTCACTTTCTTCATGATTAAATTCAAAGAGGTGTAAAATGCCGTGTATTAAGCATCTAGTTAAAAGAAATCAATTGTTACAACCAATCTCTTTAGCTTTTTTAAAGATGTATTTAGGACACAAAAAAATTTCGCCGATTGCTAGTGAAAAACCGGTTTCAGTTTCGTTATAGCACAAACTAATTACATCAGTACAACCTCTACGTTTTAAAAACTGTTTGTTCAGTTTTTTCATTTTTGAACAGGAAACAATTAAAAGCGCAAATTCTGGGTCAAACGATAGGTTTAAACTGCTTTTAATAATTTGGAAACAAAGTTCGACTCCTGCTTGGTAATTTTTACCAAAAAAGCGCTTAAAAAGGCGGCTTGAATTAATGCTAAAAGATGACTTCAAACAAATCTTTTGATGGTAACATGATAGTAATATTATATCAGCCCTGTTCTAGAGTAACACTTAAATCTACCTTTACTAGATTTTTGTTCAATTCTGATGAAATTTCTGATGTAGAAATTTTCTTTTTGTAAAGTCTAAAAGCTTTTTTTGCTGATTTTTCTGTTGTTTCTGCTTGAAAATTAGTCCATGAAACGTTGTTAAAAAGATTTTCATCTTTTTCATTTTTAAACATTAAAAAATTTTGTTGTTGCGTAGTTCAAAGATTTACATTAAGATATTGTTTTACCTTTACTGAAAACAAGATAGCAACTGTTGTTAAAAGCAAAATAATAAAAGCAATTGTTATTCCTAAAATTTTCTGCTTCATAATTTAACTATTTCTTAAGTAATTAGTTAGGTTAATCTTGTTTTGAGCTTCACTTGCAAAACTCAGATCATGCTCAACTAAGACTACAAAATTATTTAAAGTAAGTGGTTTTACTAATAGCTGATAAACACGGTTTTTAATTTCAGGTGCTATTGCATTCATGCATTCATCAAGTAAGATCACCTTATTCTTAATGTTGCTAAGTAAGAATAGTTGCAAAATTTGTTTTTGTCCTGAACTTAACTTTGTTTGGTTTTGAACACCTGCATCAAAAAGTAACTGATAGATATGTGGATTCTGATTATTAAATAAAGCCTCACTAAAGCCAAAATCATTTGCTTCTGTTTGCATTAAATTTTGACCCTTTAGATAATAAACGTTTTCAAAAAGCTGTTCTTTTGAATATCTTTTTAAATCGACGCTATTAAATTTAATATTGCCCGTATATTCAAAACCTCTACCAGTTAATATTTTTAAAAAAGTAGATTTACCACTACCATTTTGTCCAAAAATAACTGTTTCATTTTTGATTTTTAAACTAAGGTTAGCTAAATCAACGTGTTGATTAAGTGTAACTGATAAATTTTCTAGAATAATTTCATCAGGACACTTTCAATTTACTTTAATACCCTCATCCTTTTTGGTTTCTATAAACAAATTAACTAATTTCTCACGACAATAGTGATAAATGTTTATTGCAGCACCAAATTGAACAATTCTTGTAGCATAAGTTGAAAAAAGTGATTGGATACCAAAAACATAGAATAAAAAGCTCAATTGATAACGCTGTTCAATAATACCAATCACCCCCAAACCTAATATAGCAAAATCAAAACCTTGTTGGAAGAAGCTTTTAATGTTTTCTATAGTTAGACGATTAATTTCCTTCTGGTTGTTAATGTTTTGATTTTGTAAAAGTGCTTTACGAAAATTCAACATTAAGAAATTAAAGCGCTTTTTATTTTGTTCTTCACGCAAATTTTCATCTAGCTGTAAACTAATTTTGTTTTGCAATTCAACATAAGGAATTTCCTTCTTTGTAATTTTTTTAGTAAAAAAGAAGTCATAACAAAATAAGCCAGCATTGACTACTATTTGTGCAATGGCAATCAATAAAAATTCTAATTGATTAATTCCTATTAAAACACCGATAATCAGTGCAACAACACAACTAATAATTAAATTGGGAATATAAAAGTTAAAAAAGGAAAGAACTTCTTTTAAATAAAATTGTCGCTCTATTAATTGATTTGGTGATTTATTACTAAAGTAAACAAAGCTTTTTTGCTGCAAACTTTGAATAATTCAACCCAAATATCCACGGTATTGTTTTCAAAATAAAAAATTCCTAATTAGTTGCAAAATAACTTGCAAAAGCAAGTTTAATCCCTTTAGACATGAAAAATAAACTACCAAAACAACTAAATTAACTGCAGTTCCAAAATCAACAGTATTAGTTATGATGGTACGTGATGCTGTTGCTAATAATGTAGAGATACCAATGATAATCAGTTCAATAAAAACATAAAAAGTGACTAAATTAAAATCAAAAAAGTTACTGAAAAAATTACTTTTATTTACTGGTGGAATCTCTTTAAATGCTTTTGCAACAGTTGCTGCATAACCCGTTCAGAGTTTTTCTAGCTTTTCATCAGTTAAAAGGTATTTTCCTTTTGCAGGATCATATACCTCTCAACTATTTTCATGTTTGTTTTTAACTATGACAAAGTGATCTTTAAAATGAACTATTATGAATTTGCTATCAAGTTCTTTTAGTTCTTGAAATGTTAATTGATAACTGTTGATTTCAAGACCAAACTTTTTGCCATAACTTTCCATTTCAAATAAGCTTAAGCCATTTGGTGGTAAGTTAATTTGTTCTAGTAATTCATCATGAACATACTTTTCATCATGGATTGCATTGGCAAGCATACCAATTACGCAAATTCCACACTCATTCTGTTGTTCTTGATAAATGATTTTCATCGCCTTTGTTTTGTCTTATTAATAGCAATGAAAAGTGAAAAATGACAAATTTGTAAGCTGTTACAAACACTACAAACTGTTTTATTAGTTAAAAATAAAGTTACTTATTATTTATTTGCAAGCTGACTAGCACGTTCGTACAAGGTTCTCACTAAAACACCTTGTCCAAGGTTCTCAATGGAAGCAGTTGATGCAATATCACAATGGATCAAAGATACACCTTCTCTAAATTCTGCAAGGAAACAAGCTGCTCTTGAAGATCCAGCACCTCTTGCACTAGTAGAATTTTGCAAATCAGCAAGTTTTGTTAGCTGTAAAGGTTTTAAATAATCAGGGTGCATAGGTAATCTTCATACAGGTTCACCTGCACTACATGCTGCTTTTTTAAAAGATTCTCACTGGTGATCACAAGTACTGAAAATACCTGTATAGGTAGTACTCAATATGTATGACATTAAACCAGTAAGGGTTGCTACATCAATAATATGTGTAGCAGCTAAATCCTTAGCAGCATATGTAATAGCATCAGCTAAAACCAATCTTCCTTCAGCATCGGTGTTATCTATTTCAACACTTTTACCATTGTATGCTATCTTAATGTCATCAGGACGTTGCGCCTTAGCACCAGGAAGATTTTCAGTAAGAGCTGCTACTGCAACAACATTGGTTTTAACCTTGTTTTTAGCTAATGCCAAAACAGTAGAACATACTATAGCCGCACCACTCATGTCATATTTCATGCCACGCATATAATCACCTGTTTTAATGTTCATTCCGCCTGAATCATAAGTAATTCCTTTCCCTACAAAAGCAAGTTTTTCTTTGGAATTTTTATTAGCTTGATAACTAATAACAAGTAAACGTGCTTCTCTTTCAGAGCCTTGATTAACCCCTAAAAGTAAACCCATTTTTTTCTTAATTAGATCTGATTGTTTAAGAACTTTTATTTTTACAGGCAATTTACTTGCAGCCTTTTCAAAATGTTTAACAAATACTTCTGGATAAAGTAGATCTGAAGGAGTGTCTTGTAATTCTCTTGCTAGGTTAACATATTCGCCTACTGTTTCATATTCTTTAACTAAGTTGTGATATTCAACAGCACATTGAACAGCAACTTCAACATTTTTTTCAGGTTTAGTTTTCATTGTGAAAGGAGTAGTTTCACAAAAAGTAACACTTGTAATAACAGTTTTTATCGCAGTATGAAAACAACCACTATTTTCATTTTTCTTATAAAGTTCTATAAGACTGTCAAGATTAATGTTTAATTTGGTTTTCTTTCTTTTAAGAAATTGAACTAAAGCACGGTTGAAAGCAGGAAAATCAGTTGGTAAAACAACACCAAATTCAACATTAGGATTATGTGCAGCTTCTTCTTTTACACCATATGTTTTTGAACTCACAAAAACAACTGTGTTGCTATCATCACTAAAAGGTTTATTTATTCTCATTCTATATCTCTATTTATTATTCTGAAAAGGAGTTATCTTTTCTCTCTTTAACATCATACACAGCAACATTAGTAGTAATTAATGAACTAGCTACAGAAGCAGCTTTTTCAAGTGCAGTTTTAGTTACTTTGGTGGGATCAATGATTCCATTTGCAATCATATCAACCTTCTTTTTAGTCTCAGCATCAAAGCCAACACCAGTTTTTTCATTTTTCAATTCACTGAGAATTTTAACTGGGTCAACTCCTGAGTTTTGAATAATTTGACGCGCTGGTGCTTCTAGAGACTTTTGCACAATTTCAAAACCAAGTAGGATTTCTTTAATGTTTTCAACACTAGTTTCATTTTCATATCGTTCTTTTAGTTTACTGTTAGTTAAAACACAAGAAGCATTTAATAAACCAACACCACCTCCAGCGATAATCCCTTCTTCAACTGCAGCTTTGGTGGAATTTAAAGCGTCTTCAATTCTGAGTTTTAATTCTTTTTGTGCGAGTTCAGTTGCACCACCAACACGGATAACAGCAACACCTTGACTTAAATAAGCAATTCTTTCTTTAATTAAATCAGAATCATACTTGTCAGTGGTTTGTTTTAATCTTCCGTTTAGAAGTTCAACATGCTTTTTGATTTTATCCTTATTGCCTTTACCACCAATAACAGTAGTTTTTTCTTTAGCTATTTGAACCTTTCTAGCATCACCTAAATTATCAACAGTAACATCTTTAAAACCACTATTAATTTCGTTATTATATGCTAAGGTTCCACTACTAATTGCTAAATCTTCTAAAGCTGCTTTTTGACGTTCACCATATTCATTACATTTAACAGCAACAACATTAATGGTGCCCCTGAGTTTATTAACTGCTAAAGTAGTAACAACTTCTTCTGCAAAGTCAGGTGCAACAATTAATAATGGATTACCATTTTCAACACTACCTTCTAAAAGCGGAAGAATTTCTTTAATTGTGTTAATTTTTAAAGAGCTTACTAAGATTTTAGGTTGTTCTAAAACAACTTCCATTTTTTCTTGATCACTAACCATATAAGGTGATGCATATGTTCCTTTAAATTCAATTCCTTCAGTGGTTTCTAATGTTGTATTAATGGTTTTTGCATCATCAGTTGTTATCACGCCATTTTTACCAACTAAAGCCATTGCTTGAGCGATCAGTTTACCAATTTCTTTAGAACCTGAAGAGATAGCTGCAACTTGTTCTATCTCTTCGTTAGTATTAATTTTTTTAGAGTACTTTTCAAGTTCTTTAATAATAAGTAAGCTTGCATCTTCAATACCCCTGCGGATGTTAACAGGATTAGCACCTTTATTGATAATTTCAATACCACGGTTTGTCATTTCTTGTGCTAATATGGTAGCTGTTGTTGTACCATCCCCAGCAATGTCATTAGTTGACACTGCAGCAACTGAAATAACCTTAGCACCAATATTTTCAACTGGATCACTAAGTTCTATTTCTTTTGCGATTGTAACCCCATCGTTAGTAATTAATGGGTTTGCAAATTTTCTCTCTAAAATAACATTTTGGCCTTTAGGACCTACTGTTACTTTAACAGCATTTGCTATCTTATTAATACCCTGCAACAAGCGGGTTCTCGCATCTTTACCAAAGATTAATTCCTTTGCCATAATTGTTTAATTTTATCTTTTTCTTTGCTTACCACTTTCTGGTTTTTCAAAGGCAAGTACATCCTCAAATCCAATAATTTTGTACTTGTTTCCCTCATTCTCAAAAGAGATACCACTATACTCCTTAAAGTAAATAATATCACCAACACCAAAAGCATATTTTGGTTTTTCTGTTTTGCCATATGCAGGACCAGCACCAAGAGCAATTACAATCCCTTTATTAGCATTAGCATCGCTTTTATCATTACTTGCCAATGAGGTAATAATCCCTTTTTTTGAGACTTCTTCTTTGTTTGATTCCACAAGTGAAACCAAGACGTTGTCATGAATTGGCGTTATGTTCATAATAAAAATATTTTAATAATTAAAAACTAATTTAAAAGAAAGTAAAAGTTATCTTAAATTTATCTGATATTAGCGCTAAACATTAATTAATATTTAGTGAGATATTGTTAATTTTTAAATACAAATTAATAAGCTAAACCCTTATAGAGCGGAAAGCGTTTTAAGAGATTTAAAACAGCTGTTTTTGTTTGACTAATTACCTTTTGATTACCATTACTTTTAATAACCTTATCAATCAAACTGGCAACAAAAATAAAGTCATTAGTTTTAAAACCTCTGGTTGTCATTGCAGGAGTTCCAAGTCTAATACCTGAAGGACTAAAAGCAGATTTTGTTTCAAAAGGGATTGTATTCATATTCAAAACAATGTTAGCTTTTTGTAACCACAACGCAACATCTTTACCATTACCAACCACTAATGAAAATAAGTGGGTTTCAGTACCTTTTGACACAACACGATAACCCTGCTTTAAAAATCAATTTGCCATTGCTAAAGCATTATCTTTAACTTGTTGCATATACTGCTTAAACTTTGGATTCAAAGCTTCTTTAAAACAAACATATTTAGCTGCTATCACATGTTGTAAAGGTCCACCCTGACATCCAGGAAATACTCCACTATCAAGTTTTTTGATAATTGCTTGGTTGTTAGACATAATGATACCCCCCCTAGGACCACGCAAAGTTTTATGAGTTGTTGAAGTGACAACATCCACAAAAGGCAAAGGGTTTTGGTGCAAACCTGCAGCGATGAAACCAGCAATATGGGCAATATCAGCTAAAAGATACGCATTAACTTGTTTTGCAATTGCACTAAATTTTTTAAAGTCAACAGTCCTAGAATAGTTAGAAAAACCACAAATAATTAGCTTTGGTTTGTGTTCAATAGCAATTTGAAGAATTGCATCATAATCAAGAGTTTCTGTTTCAAAATCTAACGAATAAGTTACTGCTTGATATTGCTTACCTGAAAAATTAACGGGGCTACCATGGGTTAAATGACCACCACAATTAAGATCTAATCCTAAGATAGTATCTCCTGGTTTTAATAATGCTAAGTAAACTGCATAGTTAGCAGATGATCCAGAATGAGGTTGAACATTAGCCCATTGTGCTCCAAATAAAGTTTTGCAACTTTCAATGGCTAAGTTTTCAGATTCATCAACAACTTCACAGCCTTGATAAAAACGTTTACTGGGATAGCCTTCTGCATATTTATTTGTTAATACTGAACCAGTTACAGCTAATATGTCTTGGCTAACGTAATTTTCTGAAGCAATTAAACAAATGTTTTCTCTTTGACGTTGTAACTCTTTATTAAGTAAAAGTCTTACCTTTGAAAACATTCAACTTAATTAACGTAATAAAAACGGTGTTTTTTGACTGTTCTTATCAACATATTTAAATTCTTCCTTTTCACTACTTTTAATTTGGTCGTACAAGTGGGTATTGTGCTTTTTAGCAAATTGTGCATAAAAAGATTTTGTGTTACTGTCACCAAAAATTAAATCATAGTGTTGAAATTTATCCCTATTATTAGTTCTGGTTTTATTTTTTTCACTTTCACGTTTAGGAGTTCTTAAAAGTTGAGACATACCAATGTTATCTTTATGTGTTTTGGAATTCTTATATACCTCTAAAACACCAAAATAAATACTGTTAATTTTTTGGTTGTTGTTAAATATAGCACTTCCAGAAGAACCACCAGGGAGATTGGTATCATCAAGCATTAAACCATATCCATACTGTTGGTACTCTTTGTCATGATATTTAAGTTTAAAGTTTTTGAAGTTTTGAAATGTAACAATCCCTTTTTCGTGTTTAATAACTTTATCAACATGACTGTCTTGGGGTTTTTTGGAAAATATTTCACTACCTATTTGGCCATCTTTTCTGGCATTTTTTGTAATTACAGGGATCTCTTTAGCAGTTGAAACTAAATATGTGTTTTTACTCCATAACTGACTTTTTTGATTAAAGTTATGAGTTCTAGTAAACTCTTCTTTTTGTTCTAATAAGCGGTTTTGATCATAACCTCTTTCAAGTACAGGATAACCCAAAAGATAGTGGCTATGGTTTCAATGTTGTTCTAAATCTTTGGTTTCAAATATATTAGTGCTATCACCCAAACTTTTATAAGTATTAATTGCTGGCTTAATGAATTCATTGAAGATTTGTCAATCACGAAGATTTTCTAAATTAAGTTCCAGTTCTATAACAGCAAAGTCTGCATAAACAGGCTTAATACTGTTAACACCTTTCAAGAAATTAGTTGCGGTATATGCAGTTTTAGGAATATTTGATGTTTGAATAGAAAGCAGTGAATCATTTACCTTTCTTTGTTCTAATTTTTCTTCTTGATAGAGTTTTGTAATATCTGTTTTGTTAGTGTATTTACCAATTGAAAAATAAACAGTTGATTCATATTCATTAAACTCCCTTTGATCATCTGTTTTGCTGTAAGGTCAATGATCACCGATATTTCTCAACCCATCAGCAACATGCAAATTGGTTGCAATGTAAGCAAAGAAAGAACCTTGCTTTTGGGTTGTGTTTTCATCACCTTTTCAGTCAATTAATCAACCAGTACCATAAGAGTCATTACTAAAGCTAGTGAAATTTAAAGTAAAAGTTAAATCATATCCTTTTCTGTATATATCACTTTCTTCTGGGATAAAACTTGTATTAAATTTAAAGTTTCTGTTGTAAACCTTCTGTTCTACCTTAGGTTGCTGATTTACTTCTGTTCCTTGACCAGATTGTTCATCTTTAAAAGGAGTAGCGTTGTTATCTGTTGTGCTGTCGTTTTTTTCTTCTATAGGAACAGGAACAGGTGAAACAGCAGAACAAGAAGCTAAAAGTGCTTGTAATGAAATTAGTGAAAAACCTAAACTTTTTAGTTTAAACCACATATCAATCTTTAACATTTTAATACAAAAATATTTCAAAACCTTTAATTAATGAAGTTAAGAAGCACTTGTTAAACTTAGGCTTTAGCTTCAAAAATAATTGAGTTCATCTTTTCACTGATAAATTTAATTATTTTTATTCTGCTTAGTTTTAGATGCAATTAAAAAGAAAAAGCTAATGAAAAAACTAATGAAAGCAAATGCAACCATCAAAAAAGTTAAAAATGAAAATAGAACTACTACTGACTCAGGTAAACTAACTAAATTAAAACCAAAAAAATTAATCCCGTTAGAATTTGCATTGGCTTGATAACTACTATCTAAAGTTAGAAAGCCAACTACAAAGCTACCAATTAGAAGAATTAAACCAAAAATAAATCAAAAAACACTATTCAGTTTCTTTTTCATATCGAATAATCTTATCTATCCGTCTTTGGTGTCTACCACCTTCAAAATTAGCTTTGAGAAAATCATCCACTATTTTAATGTTTTCACTGTCAGTTACAAAACGACTGGAAAGACATAAAACATTGGCATTATCATGTTGTCTAGCTAAAGCTGCTGTTTTCTCACTAACAACCAAAGCCGCTAATACTCCTTTTACTTTATTTGCTGCCATACAAACCCCAACACCAGTACCGCAAATTAGTATCCCAAGATCTTTATCAGAATTTTTCTTGACCTTATCAGCTACCAAAAAAGCAAAGTCAGGATAATCATCATTAGCATCAAAATAATTAGGGCCAAGATCAACTACATTAAACTGCTTAGTCTTTAGGTGTTCACTAATAATTTTTTTAAGAGTTAAACCGGTATGATCAGAAGCAATAAAGATATTAAAACTCATTTAATTCAGTTCCAGATAGTCTTGTTTTGTATTGGTTTTAATTTTAGTTAAAAGATTAATTAATCCTTCTAATGTATCAGGATAGTTTTGTTGATTGTATAGTTTTGTTAACTGTTCTAATGATCTATCGATATGACTACTCTTTTGACGATATTTATTTGCAAAAAGAAAAGCTTGTTTGCACATTAGCCTTAAGTTAACTAAACTAACAACATCACAAATAACTTCAGTCAATTGAATTTGCAATAAATCTATCTTTTCACTAAACAAATTAAAAGTACCTAAATAGTTATTATTAGCTTCAGTGAAGATGTTATCAAGTTCTAATTTCGATTTATTAATTACTTGCAATGTTTTAGCAGGAACCTTTAAATCTTGATCGCTAATTAAATTTTCAATCTGAAAAAATTTAAATCGGAAATCATCTAAAGTATAGACAAAAAAGCGATAGTCGTCAAACTTATTAACTGCTAAATTAAGTAAATCAGTTAGTGAATTTTTCAGTTTTTCTAAAGCTTGATTTGTTTGACTCATCAAGTAAAACAGTTTTTCATAATTGATTTGATTCTGAGCTAAACTTTGATGGTTTAAGTTATTAATATTTTTAGTAATTAGATCGCAGTTCTCATTAAGCTCAATAATTAAATCATGCACATCTGTTTCATTTTGGAACACTAATTCAATGGCTTTAATTGTTGTTTTTAAAAGCTCATTTTTCTTTTCATATTGTTGAATTTGATTACGAAAATCATCAATATTTTCAACCACTAAATTAATGCTTTTTTCTTTGGAACTAACTGCTTGAAAAAGTCAACTAATCGCCTCTTGAACTTCATCCATATTCCTTTCGCATAGCATTAAATTTAAGTTATTAAGAAAATTGGCACAATCCTTCAATTTAATGGTAATGTTATTAATCAATTCATGAAGTTTTAAGAGATCTTGACTGTTAATTACTTTATTTTTCATTAATTCCATTTGTTTTACCTTTTGAATTAAAACCTTAAAGTGATGGGTTAAATAACTTAACTTAATATCAAACATATATTGCCTGTTAGCAGTAATAATTGTTTGACTGAGAATGTTATGTAATTTGTTTAAAAAAGCAATTTGCTTTTCTACATCTTTAGCTTGAATACAATTTTCTATCTCTTTAAACAAGAGCGAAATCCTGTTAAGAACATTTAAATAGAAAAGCGAATCATATTTTTCAATTAAAAAATTATTAATAAATTGGTTTAAAAAACAGAAAGCCTGACGATAAAAAACTAAACAATCATAGCTATTGTTGAAATAAACAATTGCGTTCTTAGTTAGATTAATGTATTCTTGTTGTTTTGTATATAGATGTTGAAGATTTTTATTTAAGAGCTTAATATTTCTATTAAATCAGATGAAACGATAAATATTATTTGGTTTACTGTAAACTGTTAGTTGCTCAAAAGCTGAATTGATTTCATTATCAAATTTGATTTGAAACTGTTTTAAAACTTTAATGGATTGTTCTAACGCTTGTTTTTCATTTCTTAGTAATTGAAATGGACTAATCACACAATTGAAATTGCTTAATTTCAAGTTATTATAGAGTTGTTTGGTTTTTTTTAAAAGTAAGTTATTTTGTCTAACAAAAAGACAAACTAATAGATAAGCACCAAAAATTACTAGTAATAAGAAAGGGAGCAAGATTAGAAAAACCAGCATTTTATTTTTTCTTACTTGAAAGTTTTAATAGTTCAAACATTAAATTTTGTTCAATTTTAACGTTTAGCTTAGCATCCTTGATATGGTGAATTTTTCTTTCAAGTTCTGTTTTTCTTTTTAAGGTTTCATTAATATCAACTTCATCAGCAAAAACAAAATTTTCTGTGAAAATCTTAGCTTCTGTTTTTTTAATAAGCATTAAACCAGCACCAATAATTGCTTCTCTTTTTGTTTGATCAGCAAAAATAATTTTGCACAGATGGGATTGAATAGCAGCAATCAAAGGAGCGCGATTAAAATTTAATCCTATGTAACCTTCAGTAGTTTTAACTTGCGCACTAATAATGGTTTTATCTAGTTTTATGCCACTAGGACTAAGTACCAAAAAGCGCAATAACTTCATAAAACTATTTAGTTTTATCAAACTCTTGATTTAATCTTTTTGCTTTTTCAACAGCCTCTTGAATGGTTCCAACATAAAAGAATGCTTGTTCAGGCAAATGATCATGCTTACCTTCCAAAATTTCTTTAAAAGATTGAACAGTATCATTTAAAGATACATATTTACCTGCAATACCTGAAAACTTTTCAGCAACAAAAAAAGGTTGGGATAAAAAGTTACGTATCCTTCTTGCTCTTTCAACAATAATCTTATCTTCATCAGACAATTCATCAATCCCTAGTATAGCAATGATATCTTGTAATTCTGCAAAACGCTGCAAGATATTTTGCACTCCTAAAGCGACTTTATAATGGTTGATACCAACAACACTAGGATCTAATAAACGACTTGTTGATTCTAAAGGATTAATTGCTGGAAAAATACCTAGTGCTGCAATATTACGATCCAACACTGTTTTAGCATCAAGATGGGTAAATGTTGTTGCAGGTGCTGGGTCTGTTAGATCATCTGCTGGAACATAGATAGCTTGAACAGATGTAATAGAACCTGTTTTGGTAGAAGCAATTCTTTCTTGTAACTTACCCATTTCAATAGCTAAAGTTGGTTGATAGCCAACAGCAGATGGCATTCTACCAAGTAATGCTGAAACCTCACTACCTGCTTGAGTAAAACGAAAAATATTGTCAATGAATAACAGCACATTCTGATTATCATGATCACGAAAATATTCTGCCATTGTTAAAGCAGTTAAAGCAACTCTCATTCTAGCTCCTGGTGGTTCATTCATTTGGCCAAAAACTAAAGCTGTTTTATCAATCACCCCACCTTGAATCATTTCATAGTAAAGATCATTACCTTCTCTTGTTCTTTCACCAACTCCAGCAAATACACTTAAACCAGAATGTTCTTTGGCAATGTTATGAATTAATTCTTGCACCAAAACAGTTTTCCCAACACCAGCTCCTCCAAATAAACCAATTTTACCCCCCCTAACGTAAGGAGTTAAAAGATCAATTACTTTTATTCCTGTTTCAAAAATATCAACAGTATTTGGTTGTTCATCAAAAGTAGGTGGGTTACGATGGATTGATAGCTTTGGTTTTTTTGGTAATGGTTCTTTTCCATCAATGGGTTCACCTAAAACATTGAACATCCTTCCCAATACATTTTTGCCAACAGGTACCTCTATTGGATGGTTATAGTTAGTAACTTTAACATTACGTGCTAATCCTTCTGTAGGACCTAATGCAATGCAACGAACAATGTCATCACCTATTAATTGGGTTGCTTCTAAAAAAAGCTCACTTTTTTCTAGTTGTACACTCAAACAATCATAAACTCTAGGTAATTGTTTACTTTCTGAAAAGATAACATCAACTACAGGACCAATGACTTGGTGAACTTTACCATATGTTAGGTTTTCTTTTTTTATCAATTTAATTCATACCTCCTATAACTTCAATAATCTCTTCTGTAATAGAGTTTTGTCTTAGCTTGTTAAATTGTAAAGTGTATTTATCAAGTAAATCTTTAGCATTCTTTGTAGCAGCTTCCATTGCATTTTGTCTAGAAGCTGATTCACATAACTTAGTTTCAACAAGGCCACCATACAGAGCCACATCAAAAAACTGTGGAGTAATTAAATTAATTATCGTGGCTTGATCTGGCTCAAAATCAAGTTGTTGATCAGTTACAACCGGATTTAAAGTTTTAAAAGTTTCAACATCGAAAGGAAAAACTTGAAAGAGCTGAGATTGTTGGATTAATGAGTTTTTAAATTTAGTGTAAACCATACAAATTCGATCAAGTTTAAACTCTTTAAATGCATCCATAATCTGATCAAATATCGTTTGACAATAATCAAAATTAATATCACGATCTTGGATATCAATAAATCCAACAGCAGGATTATATTGACTATTTTTATTTCAAAATGATTGGTTTTTTCTACCTAAAAAAAAGATTTTATCATCAGCTTTAAAATTAGCTTTTAATAACTTATTCATGTTCGAATTATGCTGTCCACAAAGTCCTAAAGAAGAACTCATCATTATCCATAAAGTTTTTTGGTTATCTGGTTTCTTTTTTGGTTCTTTTAAAGAAACAACTACTTGGCCAACAGCTTTATAAAATTCATTAAAAAACAAACTAATTTCTTGAAACTGTTTTTTGAACTTAATAAACTTAGCGCGTGACACCATTTTCATTGCATTAGTTATCTTAATGGTGGATTTTACTGTATTCATTCTGCGCTTAATTTCTTGTATAAAAGCCATTAATTAAGTTCCTCAATACTACCAAATTTGGTAATATCATAATCTGTTAATGTACTGATAAAACGTTTCACAAGCATTTTAAAAGCGGTTTTAGTTTTACTTTCTAAATCCTCAGTAAATTCTTTTTTGTTAGATAACTCTTTAAACAGAGGATGGGAACCATTAAATTCTTCTGTTATCCTTTGTTTAAATTTAGCAATTTCATCAACTGGAATAAACTTAATGAAAGCTTTGTTAATAGCAAATAAAAATAATGCTTCATGGACTTGAGAGTAAGGTTTACCATTTGGTTGTTTAATCATTTCCATTACTCTTTTACCATGCTCTAAAACCTTTTTTGTATTTTCATCAAGATCACTACCAAATTGACTAAAACTATCAAGTTCACTATACTGAGCAAGTTCTAGTTTTAAACTGCCAGTTTGCTGTTTAATCGCTTTTGTTTGTGCTGCACTACCAACCCTTGAAACTGATAAACCAATTTGAATTGCAGGGCGTTGTCCAGCGTTAAATAGACTACTAACCATAAACAGTTGGCCATCAGTAATTGAAATAACATTTGTAGGAATATATGCAGAGATATCACCAGCTTGAGTTTCTATAATTGGTAAAGCTGTAATTGAGCCACCACCATTTTCATCATTTAACTTGCATGCACGTTCCAAAAGTCTTGAATGTAAATAAAAAACATCTCCTGGAAAAGCTTCTCTACCAGGTGGTCTTTTTAACAAGAGTGAAAGAGTTCTGTAAGCAACAGCATGCTTAGAAAGGTCATCAAATACAATCAAAACATCCTTTCCTTTTTTAAGTCAATATTCAGCAATAGTTATTCCTGTAAAAGGACTTAAATAAACCATGGAATCAGAGTCACTAGCTGTAGCACAAACCACTGTAGTGTATTTCATTGAATCATTAACTTCAAGTTGGTGTACAATTTGTGCTACTGATGAATTTTTTTGACCAATTGCTACATAAACACAATAAACATCTTTATCTTTTTGATTAATGATAGTGTCAATCGCAATAGCAGTTTTACCTGTTTGTCTATCACCAACAATTAATTCACGTTGTCCTTTACCTATAGGAAATAAAGCATCAATTGTTAAGATTCCAGTTTCAAGTGGTTGGTTAACACTTTTCCTTTTCATTACACCTGGAGCAATTTTTTCTATTTGATCATATTCAGTAGCTTTAATATCACCTCTACCATCAATTGCTTCACCAAGTGCATTGACGATTCTACCAAGCATAACATCACCAACAGGAGTTTTCATTACACTGTGGGTTCTTTTAGCGGTACTGCCTTCTCGTAACGAAGAATAGTCACCAAAAAGTGCTATTCCGACTGTATTTTGTTCAAGGTTTAATACTATTCCTTGAATATTATTTTCAAATTGAATTAACTCATTTAATAAAGCATTTTCAAGTCCACTAACCTTGGCAATTCCATCAGCAACACTAATGACTTGACCAATTTCACTGTTAAATATTTTTTTGGAATACTTTTTAATTTCAGTTTTGATTAATGCTACGTATTCATTTAGTTTATCTGCCATAACTTCTCAATTAACTTAAATAAAATGGGCCATTATGCGTTTTAATTCATCACGCACATTTTTTTCAAATAAATGGGAACTTGATTCTATCCTAATTCCTGAAATTAAACTGCGATCAATCTCAGTTTTATAAACAACCTTTGTTTTAAAACGTTTTTCCATTATTGCAATAATTTTGTTAAGTTGTTTTGAGCTTAATTCAAAAGCAGTAATTATTTTGATAAATTGAATGTGTTTTTGACTTTCAACATTATCAAAAAAATACTTAATTGCCTGTTCAACTAAAGTAAAGTAACCCTTTAAAATAATTACTTTTAAAAAATCAACAAAAATTTGACAAAAATGATTTTTAAACAACTTATCAACAAGTCTTATTTTATCTGGTTTTGTTAGTGTATAAGAATTAAGTAAGAACGATAATGAACCATCTTTAAAATTACGCATTAATTTCAGAAAAAAATGGCACTCTTCATAAATTTTCTTTACTTGTTTTTGCTCTTCACTTAATTGAAAAAGTGCAGTTCCAAATGCTTGTGCATTAATCATCTTCTGTTTCGTTAGCTTCTAAATCTCTAATAAACTTATCAATAAATTCTCTATCTGATTTTTGATCTATTTTCTTGAGAATTAGTTCTTGTGCAGCCAACATAGCCAATTCCACACTCTCTTTAATAGATTGTTCTTTAAGAGAACGTCTTTCTTTTTCAATTTCCTGACGAGCTTGAAAAATCATTAAGTTAGCTTGGCGATTTGCTGTTTTTTCTATTTCACTTTTTAATTGCAAAGCTTCATAGTTAGCTTGATCAACAATTTCTTTAGAAACTATTAGTGCTTTTTCATGCCTTTGATTAGATTCTTCAAGTAAATTTCTTGCTTGTTTTTCTAATTCATTAGCTTGTTTGATTTGTGCTTCTAGTAAATTTTTACGGTTATTTAAAAACCTTTGAGTTGGTTTTCAAAACAAGAAAATCATCAGTGTTAGTAAGATGAAAAATGCTAGTAAATGAGCAATAAATACCCAAAAGTTAGGGAAAAGTTCATTTATTACTGAACTACTCTTAATTTCTCTAACATTCTCAGTACAAGAAACCAAAAATAAGCTGAGTGTAAAAAAGCTAAAAACTAATAAGCTTCATTTAAAGACAAGTTTTTTTGCCTTTACCATCCTTAAGCTCCTGCTACAAAAATTAAGATAAAGGAAATTAAAAGTCCATAAATTGCTGTAGATTCAGAAACAGCAGAACCAATGAAAATTAGTTTAAAAACCTGTTTTTCAACTTCAGGATTTCTTGCTATTGCCTCAACAGCTTTACCAAAAATATAACCTTGTCCAATCCCTACAGTTGAACCTGCAATCATAGTAACACCAGCACCTATATAAGCACCTAGCTTAGCACTAGCGTTAACATCCTGGGTAGTTTGAGTTTGTTGTAATATAACACCAACTGTAGCTAAAATTTCATTAACATGTTCCATAACTAAATATTTTCTTGAATTACCTTTATGCCTTTAAAGTGTAATTCACTTGCATTATTATTTTCCAAACCTTGATTGCGCATTTTAGCCCAATAATTATAAGTCAACATTACAAAAACATAACCCTGCAATACACCTGCAATTACATCAAAATAGATGTGTAATACTGGGGTTATTAAACCTGCAAAAACTGTTCCTAAGCTAAGTGCTAATGGTTGGTTATTAATACTTGAAAAAATAAAAATTCAAAAGTTATAAAAAAGCGCCAAAATAACTGTGCCCGCTAATATGTTTCCCCATAACCTTAATGAAATAGAAAAAAGCGGTGCAAATCCACTCAATATACTAAAAGGATTTGGAATGAAAGTAGAATACTTTTTTCCTTTAACAGTAATTCCAAAGGCAAACTCTTTAAAAAAATTTCATCTTTGGTATCTAATCCCCATAACAACAATCCCAATAAAAGTTGCAAGTCCTAAAGAAAAAGTAAATGTTAAAGATGATGTTGGTGGTGAAATACCACCAAGCAAGCTAACTAAGTTGCTTGATACTATGTACAGAAGCAACATTAAAAAGTAGGGAGCAAATTTCTTATTTTCCTCTCCTAAAAGATCTGCTGTTGTATCTTGTACCCAAACAAACAACATTTGAAATAAAAGCAAAAAATATGAATTATTTTTTAAAGAATCTGCTTTTTTTAACTTAAGCTTATAAAAAATAAAAAAAGTTAGAAGCAGTACAAAAACAATAAAAATACCAAGAATTTGATCAGTAGGGGCAAATGGTTTTCAACCGCTAATTGGTGAGATATCAAAAATACTTTGATTGGAAATGAAATCTATTTGATTAGTTTCTTTTAAAACTATCTCCCGTGGCGACATGTTTTCTTTTCGATTTAGATATTAAATTGATAAAAAAATATTCTAAAGGTAACCAAACAAATGATAACACTAAATTTAGTATTGGAAAAAACAATGTTGAAATTATTGTTGCAATAGTGTTAAAAATTGACGGAGTGACAACAAAAGAAATAATCACAGGAATTAAATAAATTAAATATCTAGCTGTGAAAAAAAATGCAAAGAATGAAACTGCTTTTTGCTTTTTGACATTTTTCAAACTTGCAAAGAAAGATGCTAATCTTAAAACTGATAGTGAATTTAAAACAGCAAAAGGTAATGGCAACATTCAACCAAATAAAATGTCCAAATTATTGCCTTTTGTTAAAACAACAACTGCTATTAAAATTAGTACAAATAATCAAGCTACAAAAATAATTAAGGTAATAATTTTTGCAACCTTTCTATTGTTTCAATTGAACAAAACTTTTAGATGTTAAAGTTTTTAGGTTTAATGTTTGTAAAGGTATTTCAACCTAAGTATTTTCCTTTATCACCAAGTTCAATTTCTATGTACAACAAACGATTGTATTTAGCTATTCTTTCTGAGCGTGACATTGAACCGGTTTTAATTTGACCAGTTTGGGCAGCAACTGCCAAATCAGCAATAGTTGTATCTTCTGTTTCACCACTGCGATGTGAAATTACTTGACTCCAGTTAGCTTTTTTTGCAACTTCAATTGTTTGAATCGTTTCACTAATAGAACCAATTTGATTTAATTTAATCAATATCGAGTTTGTTGTATTTTGTGCAACACCTTTTTTAGCAAGTTCTGCATTAGTACAGTAAGTGTCATCACCAACAATTTGAATATGGCTACCTATGGTTTTAGTTAATTGGTTCATCCCTTCTCAATCATTTTCACTCAAACCATCTTCTATTGAAATAATTGGATATTTTTTTGTTAATTTTTCTAAGTAAGCAATCATTTCTTTGCTTGTTAAACTCCAATCCTTTGCATTAAAGATATTAGCTTTTATTCCTTTCTTGAAAACATAAAGTTTTTTATCTTCATCATAAAACTCACTAGCAGCAACATCAATGGCAATAGCAATATCATCTCAAGGCTTATATCCAGCTAATTTAATGGCTTCAACCATGATGTCAAGTGCATCTTCTGCAAGTTTTAAGTTAGGCGCAAATCCACCTTCATCTCCTTTATTTGTGTTTAATCCACGCTTTTTTAAAAGATTTTGTAAAGCATGAAAAGTTTCACTAGCCATTTTTAAAGCTTCATGCATCTTTTTAGCACCTAAAGGCATGATCATGAACTCTTGAAAATCAATATAGTTATCAGCATGAGCACCACCATTAATTACATTTAACATTGGCACAGGTAAAACAAAATTTGTTGTATTTAATCCAATTAATTTATTTGAAATGTATTGAAATAATGAGCTGTTTTGTGCTTTTGCTGCTGCTTTTGATACTGCAAGTGAAACAGATAATATAGCATTTGCTCCTAATTTTGCTTTGTTGGGAGTATTGTCTAGTTTAATCATTGCTTGATCCACTGTTAATTGATCAAATGCATTTAAGCCAATAAGCTTAGGGGCAATAACTTTATTAACGTTATCAACGGCTTTGTTAACGCCTTTACCAAAATAATTTTTTGGATCATTATCACGTAATTCAATTGCTTCTTTCTCACCTGTAGAAGCACCTGATGGAACCATCGCTTCACCTACATGACCAGATGCCAATTTAACAACACAAGCTACTGTTGGAACACCCCGAGAATCAAAAACTTGATAAGCAAAAATATCGGTTATTTTTGAATTGATGTTTAGATTTGAACTTCCCATATTAAATAGAAAAATATTGTTAATAAAATTATTATATGTTTTAAGATTTCTATTAATTCAAGTAATATGAAAGAAATTTATTTTGGTGGTGGTTGTTTTTGAGGAATAGAAAAATATTTTCAACTTATTAAGGGTGTTAAAAAAACATCTGTTGGTTATCTCAACTCTAGGATTAAAAATCCTAGTTATGAGCAGGTTTGTTCTGGTTATACTAATGCTGTTGAAGCTGTAAAAGTTGAATACGAAGAAAAAGAAATTTCTCTTTCAGAATTAATTGAAGCACTTTTTGAAGTTATTGATCCAACTATAAGAAATAGACAAGGTAATGATATTGGAACACAATATCGTACTGGTATTTATTGAACTGATAGCAGTGATGAAAAAATAATTAATGATAAGTTCTTAAAACTTCAAAAAAACTACAGTAAACCAATTGTTACAGAAAATAAAAAAGTAGAAAATTATTATCTTGCTGAAGAATACCATCAGGATTATTTAAAAAAGAATTCAAACGGTTATTGCCACATCAAATTTGACTAATTAAATATTTCAGGATTTTTAATAAATAAAAAGGATTTAATGATGTTATAGCAATAATCATTAATCCGTTCAATATGTTTAACAGAAGCTACAAGATCAATTAAGAAAATCCTTTCAGTAACCTCATTTTTATTAATGGTTTCATAGTATTTTGTTAATACAAGGCGATATTGGTGCTCAAATTCAATAATAGTTTTGGTTGCATTTTCAAATTGTAATGTAAGTGGCTTTTCTTTATCTTGTAAACTTTCAAAGGTTTTCTTAAGGTTATTGAGCGCACTTTGATGCAAAGTAACTAGATTATTAAAAATCTTTATATCAAGATGCAAATAATGTTCTATAAACTTAGTTAAATTATTAGCATAATCACAAATGCGTTCCAAGTCACGACTTGACATAATTATTGTGATTGTTAGGCGTAAATGGCTAGCTAATGGAGAATTTTTAGAGATTGTCCAAATTGATTCATTAATTAATTTAAATTCAGACCTATTAGATTGGTCCTCCATTTCATAAATAGTTTTAATCAGTTCTTTGCGTTTTTCTAAATTATCTTCACAAAGTAATTGATTTAATGTTTCATGTGCATTGATTACGTGCTTAAAATACTCAAAAAAAAGCTTTAATAATTTCTTTTCTGAACGCTTTAAAATTTGGTAATTAATGTTTTCCATAGCTAATTTTTCCCACTTATATAACTATTTGTTGCTTTTTGCTTAGGCTTTGTAAATATCTGTTTTGTAGTGCCTTGTTCAATTACTCTTCCATCAGCAAAAAAAATCGTTTCATCAGTTATTCTAATTGTTTGAGCCATAGAGTGAGTAACAATAACAATTGTGAATTTTTCTTTTAGTTGTTGAATTAGAAGTTCAATAGAGTTTGTGGCAATTGAGTCTAAAGCACTGGTAGGTTCATCCATCAAAAGAACATCTGGTTGTAAAGCAATAGCACGCGCAATACACAAACGTTGTTGTTGTCCACCAGAAAGGGTGTTTGCATTCCTATGTAAATTATCTTTCACTTCATCTCACAATGCTGCTGATATCAATGCCTGTCTTACTATTTCATTGATAGCATTTTTATTGTGAATACCATGTGCTCTTATGCCATAAGCAATGTTTTCAAAAATAGAAAAATTAAAAGGAGTTAATTTTTGAAAAACCATGCCAACACTAGTGCGCAATGTTAAATCATTAATAATTCCTGAATTGATATTCTTACCAAGAAAATATATGTCACCTGTTCAACGTGTGTTTTCATTTAAATCATTTAATTTATTTAAGCACCTAATAAAGGTGGATTTACCGCATCCTGATTTACCTATTAAAGCAGTTATTTTATTGCGTTTAATGTCAAGATTGATATCAAATAATACTTGTTTGTTTTTGTTATATCAGAAGTTGAAATTTCGAATTTCAAATACATTTTTTTCATCAAAATCCTTTTTAATGACATTATCTTTGGTGTTTTTAATCTTTATTAGACGGTTTGAAATTTCAATTTCATTCTTAATACGGGAGATTTCATTTGCATCTGTTTCATTATTTAAACCAGTAATTTTCTTTTTATATTCTTTGATTTGTTGTTCATAAAGCTTTCGGTAATGCTTAATTTTTTCAAGCTTTAATTGAAAATTTTTTCAAAGTGCTTTTATGTTCTTTTCCATAATTTAAAGGACTGAAATTTACTTTTAATTACTAGCCATTTTTGTTTATTTAACAAAAATAAACTCGGGATTAAATAAGAACTGAAAAAGATTAAAAGAATTAAGAAAACAACAGAGACCAATGATGTTTCTAACATAACACTTATTGCATTGCTATTAATAGAAAATAACTGTCCATATATCCTTGTTGTTAGTGTTTGACCTGGCAATGACAAATGAAATAAATTACTAGATGATAACCCTGAAGTGATAAAGAAGGGTGCAGTTTCAGCAATAATTCTGTTGATTGACAAAATTAATGCAACTATTAATCCTTTCAAAGCACTAGGTAAAACTATTTTGAAAATAACTTCACGTTTACTTATACCTAAAGCAAAAGCACTAATTCTTAAATCCCAACTGACATTATTTAGTGCTTGTTGACAAGTTCTTATAAGGAAGGGTAATATAACAACACTAATAGTTAGAATGCCTGCTATTAAACTAGTACCATTAGCTCCTCCAGCACTTAACTGCAGGACTCTTAAAAAGAAAGAAAGTCCAAATAATCCATAAATAATAGATGGCATTGAACTTAGTGAATCAATTACAAAGTTAAAAACATTTTTAACCACTTTTGAATTATTGTACTCGTTAAGTCAAATTGCAATTAAAAGTGCTAGTGGAAAAGTAATGGTGATAGTAATCAAAATAATTACTAGAGTATTTACTAGTGCTCTGCCAGTTGAATCAGCTTCAAATGAAAAAACAGTAGAACCATTATTATTAATAGCAACACTTCCATTAATAAATACAAACAGCAAAATTGCTAACACAAATCCAAAAGCTAAAAAAGTACAAATTAATTCCTGGAATATTTTTAAAAAATAATTAAGTTTTATTCAAACAACACTTTGAAGCCTTTCTTTAAAAAAAAGTGAACGTTCATTGATGTTGTTTACTTTTATTTTTTTAACACTTTGTCTTGTTGATGTTAAATCAACAAAAAGTGCACTAATATTATTGGGAATGAATCACACAACTTGATAAATAAAATTACTAATCTTTTTTAAAAAGGGATAGCGTTCCAGTGTTTTTGGATTAGCTGATCAAATGGCAAAGAAATTTAACAATGAAACTAATATCAAAATTATGATTCCAAAGATATATAAAACACCATTAATCTGTTCATCTCCATTTTCTGAAAAAATAAAAGTGGAAATAACTGATCCCAGTGTTTTTAAATCAGAAGTAAAAAAACGATTGTTGTTAATTACCTCTTGATAGTTAACACTCTGCAAAACAAAGTTAACAGCCATTGTTTCACTAATTGCTCTCGCAAAGGCTAAGGTCAAAATAACTGTTAATTGTGGTTTAATTTCTTTTTTAATAATTTTGTAGATCGCACTTGTTTTATTTTCCCCTAAGGAAACAACAACACTAATTAGATCGTTATTTACATAAGTTAATGTATTTGTTGTTAATGAAATAACAATAGGAATGATCATAAAAGAAAGCATAGCTATCACATTTAAAAGTGAAAGCGGCGGTAATTTCAAGATATCCCGAAAGAAAATGCTTAATATTTGTGATGCAAATAATCCAAAAATTACAGATGGTATTCCTGAAAGGATATCAATAATAAGTGAAAGTTTTTTTCTTATTTTTGGTTTGCATCGATAAACAAGGAAAAATGAAGTTCTAACCCCTATATAACTAGCAATAATTAAAGCTCCTATTGATACAATAAAACTTACCAATAAGGGGAATCAAATTCCTGCCTGTTTATTACCTAAATTAAATTCAAGATTAAACAAGGACTTTGCAAAATCAGGTCCAATTTTTGTTGCTTCTGTTAAAAGAAAAACAAAAAAACTTATAAATAAAACTAAAAACAAGAATGCTAAAGTCTTTGAAAAGATTCTTAAAAGATTCTCTTTTTTTAAACGTTTTTTAATCTTCTTTTGCACTAAAAAGCTCCAAAGATAGGATTACTAAAAGCAAAGTCATCAACTCAAAAATTTTCTAAATTCATTGGTGATGAGTCATTTGTTTTAAACATCTTCTTTTTCTCATCTTCAGTTAATTGCAAAATACCAAATTCATCATAAATATTTTTAATTACATCTTTTTTTCCATTAAAAAATAATCAATTAAAAAAAGTTTTAATTTCATCAATATTCTTATTCTCTTCAGAAAGTGAAACTATTGAATTTAAAGGACGTACTCATTTATACTCATTTTGGGAAACAGTTTGATTAGAAGGCAAAACTTCCTTATTCTCATATTTAATTGGTAGTATCTCAAAACCATTATTTTTAATAGCTTGAAGGTTGTTTTGAATAAATCCTAAACTAAGGTAAATCATCCCGAAAAGATTAGGATCTTGTAAGTTAGTTACAAAAGTATTAAAAGCTTCAATATTTGTTTCACTAGTAGGTTTAGCAAGAGGTCCATAATTAATAATTCCTTTCAAAATATCTCTAGTTTGAGAATCTAAAGTTTTATCTGATTTTAAGGCGGAAAAATTTAAAAAAGCTTCTGCTGTTCCTGAAGCAAATGACCCACCAGTTCTTGGAAAACTTGTTAAAGGGTAATCTTTTTCATTTGACATATTTTTTGTATCCTTTTTTACAAACTTATCAATGTTAATTGTTTTAACACCAGCAAATAAGTCGTAGAGATCATTTATATTATCTTTTGTTAAAAGTAACTTACCTTTTAACTCCTGTGGTGCCTTATAAATTACTGCAATAGCATCTTTGCCAATAGTTAAAGTTTTCAGCTTTTTATCTCTTCATTTTTTTTCATTCTCTTTTGCATATTCCTTAGGGTTTTTAGAGACATTTCCGATATCAGCAAAGCCGTTAATGATTGCTCTGATTCCAGCATTTGATCCGCCTGCTTGCACACTAATTTCAACAAGCTTATCATTGTCATTATTTTCATTTAGAACATAATAAGAACTTAATTTATTTAGTAAAGGTTGAACTGAAGAAGAACCAACAGCACTAATCAGGTTTATGTTAGCACAACCACTAAGAAAAAAAGCAGATGCTAATGTTAGTAGCGTCAATTTAAAGAAATTTCTAAACTTTAGCACTTTTTAAACAGATTAAATCTTTTCTAAAGAGAATTCGAATTGACACTCAGGTTATTATTTAGTCTAGTTTTTTCACAAGCCAATTAATTTAAATGAAATGCATTTTTATTAATTGCAAATTTAATTTCTTTTCTTAACAATTTTCTTTTTTCTTCATTTTAAAAACAAAACAGCAGAACTTATCATTAAGCTAATGATAATTCCAAAAATGACTCATAATATTAACTCAACATTTGTATCTTGTTTTTTAATTTCATTTTTTTCTAACTTATCAACTTCATAGGATTTATCTTGAGATTTAAGATTAAAATCTGGAACTCTCTTGAATTGCAGGTTATTTTGTTTTAGATAAAATGATAATGCTTTGAAAAGTTGTGTTTGATTTTCACTAGTAATATCAAATCAAATAGAATTATCAAAACCATTGGCATCTACAATTAAAAGTGTTTGATTATTTGTTATTTTTAAATCATAGCTAATACTAAATACTTGGTTATCATTATTAACACCTTTAATAAATAATTTTTTTGATCTAACATCGACATCAAAAACTAAATTAAATTGATTTTTATTTTCTACTAAATGGATGTTAATAAATTTATTCTTTAGGTTAAAGCGGACGTTTTTAGTTGTTTGCAATCTTTTTTCACTTAGAGTAATATTGAGTTTATCAACTAGTAATTGTTCGTCATTTAAACTAAAAGGTTCATGAAAATCAAGTTGATAATCCTCTAGAAATTTAAATGAAGATGACTTTAAATCTAATTCAGCAATTTTTAAGTTATCAACTGATAAAAATACCTTGTCTTTTTCTAACTTAAATCCCAGGTCATTTTTATCAACAAAAAGTTTGTTAGTATGAAATTTAAATAAATATGTTCTTAATTTAGGGTTTTTTTGATTTTGTGATAGAAAATACATTTTGTCTTTAATAATTTCAGAATCACTTTCAGAACCATATTTGAATAGTTCATTGCTATTATTTATTTCTGCTAAAACATAAAATATACCTTTCTTTTCATTAGTAGGCTCTAGATGTTTGAAATATTTTTTAGGGTTATCCACAAATGTTAAAGAGTTACTGTTGATATCTAAATCAATATTCTTATCCTTAGATTTAAAACTATTTTTGATAATGAAAGCCATTTGTCTTTTTTCATAATCATTAAACTTTTCATTACGTTTTTTAAGTTCAGCAACATTAAAGTTTTGAACATTTTTATTTAGTGGAATACGAAAATCTTTAGCAATTCAACGCTGTTCATTAAAATCTTTTCTATATTTAAATTTGTATTCAAAACCATCCTTAGTTCTTTTGATAGTTGAAAGTTTTTTAATACTGTCAAACAGTGGTTCAACACTATAGTTACTAATATTTTCTGATTCATATCCATCTACAAAAAAATTAAGTGCTAAAAATTCAATATTCATTTCATTATCTGCTTTCTGTTTTTCTGTTAAAAACTCCTCAATTGAACTTAAATCAAAATAATTCTTGTTATTTCTTAACAATGTTATTTCATTTACAGAATTACCAATTTTTACTTTAACAGTTAATGTTGATCTATCAAAAATGGTTTTGACCATTCTTGGTAAATTTCTAATAAAGAATAGTTCTTCTTTATTGTTTGGTGACATTTGCAATAGGTTGTATAAATAATCTAAATTTGGATAAAAATTACTTTGATTATTGGTTGAAATATTAAAGCTTTTTAACGCTTCATTTTTATCAATTGATTCATCAATTCTTTGATTAATTAAACTAACTTCTTTAATTGCATTTATGAATTTATCTTTAAATTTAATAGTTGGTTTAATTTTTGTACCTATTGTTTCTTTATTTAGAGTTTTTTCAGTTTGACTAACAAGCGAATTAATATCAAATTCCAACTGTGAAATTCAAAGCTTTGAAAGATTACTGAAACTAATTGGTAAAACTTCATCAGGATTATTTTGCCTTAGATAAAAACTTATTTGATGATGAACAACTTCTTGTTCAGGTAATATAGGTGTATGTTTAAAAATATCCCATTTACTGTTGAATGCTTCACGTCTACTATCTTCAGTTTCTGGTCTAACTACTAATCCATTACCTAGTCTTAATATCGTTTGAGTTATTTCACGATTATTAGTAAATGCTTCAATGATTTTTCTATCCTTTTTCATTTTCTCTTTAAACTCATTTATACTCATCCAATGAGCATCAACTTGTTTGTTGCTGTTAATAGAAAAAAATCAACTGCTTCTACCAGGAAAAGGTTCGTTTCACTGAGCGTTATTCTGTTCAAAAACTGGAGGAACTTTCACTAGTTCTAAATCTCATCAGAAACGATAAGCTCTAATTGGTGTCATTGCTAAACCTCATCAGAAATCTGGAGCTACAAAAATATTATTTTTTCAGTCAGCAAAAAATCCTCTTGATTCTTGGCCAAGATAATATCACATAGGTGCATTAAAATCAATTACATAATTACCATTATTACCTTTTCTTAATCATTTAATATTTCTAATAACATCTTCTGGGGTTGGTTTAATCATTCTTAAAGTCGATCCTCACATGTGAACAAACTTAGCTTCAATAAAATATGCTTTTTTATGACTGGGCTTTGTAAGGTCTCAAAAATAAGGATTATCAGGTTTATATCAACTTTTGTTTCAAGAACCATCTGGATTTGTACCAGTTATTTCTATATTCCTAGAAGGAACTTCGGTTCATTTTTCATTAGTATGACCATCTCTTGGAAAAAAATTAGAAATTGCTTGACTATGAAAATAAAATCTCTGCTCACTATGGATATCAGATGACCATGAGTTTCTAATTCCAGGGTATCTAAATGTAGATGAAAATGCATGTGCAATATAACTACTCTCAATTTTTAACTTTATGTTCTTTAGATAATAAATATTGTTGTATCAGTGAAAGTTATTATCTCTAATAATTTGTTTGTAATTATCACTATATTTATTAACTAATTCACTTCATTCATTCGAATAAGTTTTTGCAAAATAATTATTTTGATTAGGTCTTTGAATTACTTCATTATCAAAATTAAAGCTTTTTCGAAAATAATCGTTTTTTAAATTAAATCCAGCTGTATAGCTTCTGTTTTGAATTGTGTTTGGAGAGAGATAAAGATTAGTATCTACCAAGTTTTTTTCAAGTGAAGCATTAGCTAAAGCAACGGGAAAAAGAAAAGAAACTAATAAAGGAATAAAAAGATATGAACGCATAATTTATCTCATTAAATAAATGTGTATTTAATGAAATATTGACAATTTTTATTGCTAGTTCTTGTTAAAAATTATCTCTTGTTTTATTTTTTATCATCTTTTGTTTTTTATTTCACTTATGAAAGTAAATAAAAACAGTGATAAACAAAACTAAAACACTAAAACCAATAATCAAATATACTATAATATCTGTTTTTTTATCTTCACTATTAGATTGAATAATTTTGTCTATTTGAAAACTATAGCTGTTTTTCTTTAAGCTAAAATTAGGTTCTTTTTTAAATTGAAATTTTTTTAATGAGAGAAATAACTTAAGCTCATTTTTCAGTTTCTGATTATTTTTCTTATCAAGATCAAACCAAATATATTTATTTAAGTAGTTATTTGTTGTTCTAATTTGCAATTCATTTTCATTTTTGATAGCAATATCATAAACAAGGCTAGTTAATCTATTTTTACTTATATTAACTTCAATATTGAGCTGATTTTTAATTGCATCAACATTTAAATAAAAATGAAGCGAACTATTTATCTTTAGAGTAGTTAATTTGATCTCTTTAGGAAAAAGATCTAAGCTAGATTTCTTTTTATCTATTAAAAGATTCATTTCACTTAAAGCCAAATTTAGTTTTTTAATTAATAGTTCTTTATTAGCTAAAGAAAAATAATCATTTATATGAACTTCATAACTTTCAAAAAATGAGGTATTATTTTCTTTTGGATTAAATTCATCTATTTCAACATTATTAACAATTACCCTTAATTTATCACCGCTTGATTTAAATCAAATATCATTAACATTTAAAACTAACTTTTTGGTTCTCATTTGAAAAAGATAAGTTCTTATTGCAGGAAAGTTTTTATTTTGTTCTAAGAAATACATCTTATCTTTAATGATTTCGCTATCATTTTTAGAACCCCATTTAGTTAAATCATTACTGTTAATTATTTGTGCTGAAACATAGAATATACCATTTTTAACACTGGGCTTAATTAAAGATTTTTTAATTAATTTCTCAGGATCTTTTATGTATTTTATTGTAGCGGATGAAATATTTAAATTGTTTTCTTTTTGAGAAGTAAAACTATTTTTAATTGCATATGTAATTTGTTCTCTTTGATAACTATTTAATCCGTTTTTTTCAATGTCATTAACACTGAAATTTTTCTGATTTGTAACAATAGGAATTAAAAAATGATCTTTTAAAATATGATGTTCATTGTATTCAGATCTAAACTTTAAACTATATTGAAAACCATTTTTAGTTTTCTGAATAGAAGGCAAATTTTTAACTGACTTAAAAAAAGAATCAACTGGTTGATTTTTTAATGTTCATCCTGTTTTATTCACAACAATAAAATCAAAAGTTAAAAACTCAATTTCATAAAAATTAATACCAGTTTGATCAGCATTTAAGAAATTTTCAACATCACTTAATAAAAAAACGTTACTGCCATTTTCTATAAAAGTAATTACTTTATTTTTGCCACCTATCTTTATATTTATCGTGATGGTAGTATCTTTAAAAATACTTTTAATTAGGTTGGGTAATATCCGAATAAAATTTAATGTTTTCTCTTGTTTTGGATTAATGTTAATTAAATTACTTAAGAAAAATATTGCATCAATTCTTTCATCTTTTGGTTCAAAATCAAATTTAGATTCTTCCTGTTTTTCTAATAAATCTAAAGCAAATTGAAAAAAAGTTTTATCTTTATTTGCTAATTGATCAAACTCTTGATTTAGCTTACTTACCATTTCAATTGCATTAGAAAATTGATCACTAAATTTCAGCTTTGGTGTTATTTTTGATCCTATTAGTTCTTTGTTTAAAACAGTAGTTGGATTAGCTATTAAAGAATTTGAATCAAATTCAATTTGGTTAACAATGATCTTAGCAAGTTGATTTGTTGCTATAGTTAATTGATTTTGTGATTGAAAATCATCTTTAACTAGATAAAGATTAGTATCAATCAACTTACTTTTTATGGAAGTGTTAGCAAATAATAAGGGAATTATCGCAAAGGAAACTCACTTTAAATAGCGTTTTCATGACATAATAAGCTTTATATTCCATAAATTAAATAAAACTTATTAAGTGACAGTTTTTTTACTAACGCTTAGTAAATTGTGGTGCACGTCTAGCACCATATAAACCAAATTTTTTGCGTTCCTTAGCACGCTTATCACGTGTTGTTAATTTTTTGGTTTTTAATAACTTTTTAAGATCTGGATTAAATTTTATTAAAGCACGAACAATACCTAATCTAATGGCTCCTGCCTGCCCAGTAAATCCTCCACCTTTAACAACAACATTGATATCAAAGTTATCCTTAAGTTTGGTTAACTCTAAGGGTTGTTCCATATCTTGAATCACCAATTTATTTGGAAAATAATCGCTAGGATTACGATGATTAATGGTTATTTTACCCTTATCTTTGCTTTGATATAAATAAACTTTAGCAGTAGAAGATTTACGACGACCAAGTCCATAAAAAGATTTTTTATCCATATCTTTTAACTTCAATTAAGTAATGTTGGTTTTTGTGCTTCCATGTTGTGCTTATCATTCTTAAAAACATGTACTTTAGTTATTAATCTTCTGCTTAAACGATTATCAGGTAACATTCCCTTAACAGCATTGAAAACAAGTTTATCTGAATTTTTGTTTATCATATCCCTTCCACTAGTTTTTTTAATTCCACCCATGTATTGAGAGTGATGATATCAAAACTCATTGTCTTTTTTATTTCCAGTTAAAACCACTTGATCACTGTTAATAATTATTAGATGATCTCCACAATCTTGATTAGGAGTAAAATTAGCTTTATTTTTTCCTCTAATTAAATTTGCAGCTTTAACTGCTAATTTGCCTAAAACCAAACCACTAGCGTCAACAAGATACCACTTCCTGTTTTTAATGGCTTCTTCCTTTGTAAGCATCGATGTTTTTTGCATATATTTAGATACAAAACCGCATTCTATTTTAATATTATTACTTTTCTTCAAAAAGTCCTTTAAAGTATTTATCCACCATTTTTTGTGATAAAGAGCGTTTCTTTTCTTTTAAAAAAGGCTTAGCTAAGTTTTCCAATTGGGTTAAGTTCAACACTTCAGTTTGATACTTTTGATTTTTTGTTTCTTCAGTAATTGTTGTTTTATATTGATGCTCACTAAAAGATGCTAAGAATAAATTTTGGTTAAAAATCTGAAATATAGTTGCTTGTACATTACCTTCTTCTATTGCCTCTTTTCAAAGTCTCATAGCAATTTTTTGATCTTCAAATAAGATAACTATCCCAAATGAAGAAGCTTTTACTATTTTGGTTGCTTTCACTATATCACGAGCACTATATTTGTCTATAAAGTTATTGAAATAACTTAAATTATTCTGTTTATCTTTATCATTAAATTTAGTTTGAAAAACACTTAAAAGATTTTGAAAATGATCAATTCATTTATTGTCATTAAATGCATTAATTGCAAGAGCAATATTGGCAAGATTTAGTTTGCTTGTATCTAAGAATTTGTTATTAGTAACTTCATCATTTTTAGGTGTCTTTTCTGATTTATCTATAAAAGATTTTTCAAATAAAGAAAAGTGCTTTTGGTCTTGTTTGTGATTTGATTTTTTTATTTTTTTCTTTTCCAAATATTCAGTTTCTAAGTGTAGAGATTTTTCATTACTTGGTGAGCTTATTGTTAAAGGTTTTTGATTAAAAAATTCATTATAGCTAATAACAATAGCTTTTAGCCTATCAATTAAAGTTAATTGACTTAAACCAAAATTAGTATTTTCTTCAATGGCCTTAATTAAAAACGCATAATGCTGCTTTTCAAAATTAAGTGTTTCTATCATTGTTTTTTCAGTTGAATCTAAAACATTAATATTTTTTAGTTTTGCATACGCATATAAATTAACTGTTAGCGCAAACAACTCTCTTAAGAAGTAAGTGAAATTTAGACCATTACTTTCAAAATCATCAAGCAAGTTAAATGCTTCTTTTATATCTCCTGATAAAACTGCTTTAATAAAAGTAAATTTTGCATTTCTATCAACGATATTAAATGTTTTTTCAACATCAGTAATACTAATTTTTTCACTATCAGAGAAATTGCTAATTTGATCTAGTAAGCTAAGCCCATCACGCAATGAACCTTGGGATAAATCAGCAATTTTTATTAATGCATCTTTCTCTATCTTAATCTTTTCTTTTTTTGCTATATCATTTAATCTTTCAAGGATTAAATCACTAGTTATTTTTTTAAAAAAGAAGCTTTGACATCTGGACAAAATTGTTAATGGAATCTTGTTAAATTCAGTAGTTGTAAAAATAAAAAGAACATAAGGTGGTGATTCTTCTAAAGTTTTTAACAAGCCACCCCATGATTGGGTGGTTAACATGTGTGCTTCATCTAAAATATAAACCTTTTTTTTAAATGTGAAGGGATGATTGAAAACATTTTCTACCAACTCTCTAATATCATTAATACCATTTTTAGAAGCTGCATCTATCTCAACTATATCAATGGCACTATTAGTGTTAATACTTTTACACACATCACAACTATTACAAACATCAATTTGATCTCAATTTAAGCAGTTTATCGCTTTTGCTATTATCTTTGCAAAAGTAGTTTTACCTGTTCCTCTTTCACCTGAAAAGATATAACCATTAGGTAGTTTATCCCTGTTAATAGCATTCACCAAGATTTTTCTTATCGATTCTTGTCCTAGGGTTTGTTTGAAATTGATTGGCCGATATTTTTGATAAAAAACTTGGTGCATAATTTTTAAATTGAATCTGGTTTTAAAATTTTGGACAAATATTGGGCAGTATAAGATTTATTAACTTGGTTTATAAGTTGTTCAGGTGTTCCTTGAGCAACAATTTGACCACCATTGTCACCACCTTCAGGACCTAAATCAATGATATAGTCAGCAACCTTAATAATATCTAAGTTATGTTCTATAACAACTACTGTATCACCATTCTTAATGATTCTTTGAATTATTGTTAATAGTTTGTTTATATCTTCTAAATGTAAGCCAGTAGAGGGTTCATCTAAAACAAACAAAGTTTTACCAGTAGATTTTTTTTGTAAAAACTTAGATAACTTAATTCTCTGTGCTTCCCCACCTGAAAGAAAAGTGACATTAATACCTAATTGCAAATATTCTAAACCAACATCACATAACAACCTTAGTTTACGTGATATATTTGGGATAGCTTTAAAAAATTCATAAGCTTCTTTACAAGACATTTGTAAAACATCAAAAATTGATTTTCCCAAATATTTAATTTCCAGTGTTTGTGAATTGTACTTCTTGCCATTACATACTTCACATTTGACATAAACATCAGGTAAAAAATGCATTTCAATGCGAATCACACCATCACCAAAACACTTATCACACCTACCACCTGGAACATTAAAAGAAAATCGTGAATTTGTATATCCTCTTGCTTTAGCTTCTTTTGTGTTGGCAAATAAATCACGAATATCATCAAAAACACTAATATAGGTTGCAGGATTAGAACGTGGTGTTCTACCAATTGGGTCTTGAGAGACAACAATTATCTTATCAATGTTGTTAGCACCAATTATTTCCTTATATGTATCTTTTTTAACACCTTTACGATAAAGAATTCTTTCTAAAGCTGGAACTAATGTTTGATTAATTAAAGAGGATTTTCCAGAACCTGAAACTCCTGTTATCAAAACCAATTTATTTAAAGGAATCGTAACATTAATATTTTTCAAATTATTAACTTTAGCACCCTTGATAATAATTGTTTTACCATTACCACTATGTCTATTTTTTGGAATGGAGATTTGTTTTTTACCACTAAGATATTGTCCAGTAATTGAGTTTGAGTTTTCCATTACTTGTAAAGGTGTACCGCAAGCAACTAATTCACCACCTTCATTACCTGCTTTAGGACCAATATCAATTAAATAATCTGCCGCTAACATTGTTTCACTATCATGCTCAACTACTAATAAGGTGTTACCTAAATCACGCATTACCATCATTGTTTTAATTAAACGCATATTGTCTTTTTGATGCAATCCAATAGAAGGTTCATCCATTACATATAAAACACCAGTAAGTTGAGAACCAATTTGGGTAGCTAATCTAATTCTTTGTGCTTCTCCACCTGACAGCGTAGAAGCTCTTCTTGCAAGATTAAGATAATCTAAACCAACATTTTTAAGAAAAGAAAGACGATTAATAATCTCTTTTAAAGCTAATTCACCGATCTTCTTTTGCTCATCATTTAACTCTAGTTTTAATAGAAAATCAATACTTTTATCAATGGAAAGTTCAGTAAAGCTAATAATGTCAATTCCTCCTAACTTAACACTTAAAGCGTCTTTTATTAATTTTTTTCCATGACACTTTTTACATGTTATTTCAGACATGTATGCAGAATATCATTCTCTACTTACTTGGCTGTTTGTTTCTAGGTGTCTTCTTTTAATTAAATTAGCTATCCCTTCATAATGCTCAAAGCGGATATTCTTAGCACCTGAATTGGAAATTGTTTTTATTTCAATAGGTTCATCACTTCCTTCTAAAATTAAATTAAGTTGTGACTTATCTAACTGTTCAATTGGTTTATCTAATGGAATTTTATAGTGATTAACTAAAGATAAAAAGCGCTGTCAATCCAAAGAAGTTCCATGCACAATATTTTTAAAAATATCAATGGCTCCTTGGTTAATAGAAAGTTTAGAATCAGCAATTATCTTGTCTACATCAGGTTCATAACTAAAACCAAGTCCTTTGCAATATGAACATGAACCTAAAGGGGAGTTAAAGGAAAATAATCTTGGTTCCAATTCACTAATAGAAAAACCACATTTATCACAACCATGATTTTTGCTGAAATTTAATATTGTTCCATCTTCCTTAAGAACTTCTATCTTGCCATTAGTTAACCTATCAATGGTTTCAATGCTATCAACTATCCTTGAATAAGTTTGATTATCTTTATTGATGATAATTCTATCGATCACTACACTAATGTTGTGTTTAGTATTTTTATCAAGTTTAATTTCATCATCTAAGGTGTAAATCTGGCCATCAACTAAGACTCTAAGAAAACCTAATTGCTTGTATTTAATAAATTCATTTGTAAAAATGCCGCGCTGATTTTTAACAGTAGGTGCTAATAATTGCACCTTTGATTTATTAGGTAAATCAAAAATCTGATTAGCAATTTGGTTAATTGTTTGCGTTTGAATAGAACCATGACCATTAGGACAATAAGGGGTCCCAATTCTAGCTCATAAAAGTCTTAGATAATCATAGATCTCAGTTACTGTACCCACAGTTGAACGTGGGTTATGTGAAGTGGTTTTTTGATCAATGGAAATTGCTGGTGATAATCCTTCTATAAGATCAACATCAGGTTTATCACTGTTACCTAAAAATTGGCGTGCATAAGAAGATAGAGACTCTAAATATCTTCTTCTCCCCTCAGCATAAATTGTGTTAAATGCTAAGGAAGATTTACCTGATCCTGATAGACCAGTAATAACAACAAATTGATTTTTAGGGATATCAATGTTAATGTTTTTAAGGTTATTTTCTCTAGCACCTTTAACCCTTATAAAATCATTATTTTTTCATTCCGGTTTCAAAGTCAATGTTTTTAATTGAAAAATTTTTAACTAACTTATTATTAGGATCATTGAAAATAAGCGCAATACCTTGTTTATTTTGATATGAGATCAAAAGTCCACGAATGAAATTATAAGCGTGGTTAGTGAAATCATTGCAGCAGTTTATCATTAGTAACTTAGGATTTAACAAAAGTTTAAATAGCAAGTAAAGCTTTAGACTTTCATTTTTGTTAATTTTGTTTAAATCACTAAATAACATTCAGCTGCTAATACCATTCTTTTTGGTTTGATTTATCAATAAATTAATGTTGTTAATAAAGTTAAGTTTTTTAAGATTTTTTTGTGATCTTAAAAAGAAATTAATTGTTTTTAATTGTGAATTCAAAACATTAAGATAAAGCTTATTTGAAGCTTCTAAACGATTGATATTGCACTCTTCTGAATCAATAAAAAAAGTTCTCTTAATTTTCTTGTTTGTTTTTTTAAGTCTTAAGTGTAATGTTTTGTTTAAAAAACAATATCTTTTCATGATGAATTTAATCTTTTGTAACTTTTGATTTGTTTCATCGCGAAAAATTCACTTAACTAAATTGATGTCTTTTAAAAGTAATTTGTATTTTTGTTTGCATTTTTTTAGCTTTTGTTTTAGATCATTCTTGGTTTCTTTATTTGTTGTATCTGTTGTTGAAGAATAACCGCGTGATGCTAAAAGGAAACTAACAGGGGTTTCAAAATTTAGATCTAAATATTTATTAATAACTTGGTCAACTAGAAAATTTCACTTTGGGGAGTTTAAATCATTTTTTAACTTATCTTTTAGCAAACTAAAATGTGGTTCACTACGTCCTTCATTTCAAATAAAATCATTAGAGCCACAACTTAAAAACTCATATTTATTAAAGAATTGTTGAAATTTTTTACTTAGGCTATTTCAAAGTGAAATAAGAACAAAATACAGGTTATTTAACTCATATTTTTGTCAGTGAATTTTTTGGTAATTAGCCAATAAGCTTTCATAAAAATATTGCTTTATTTGAAATGCAAAGTTAAAAGTCTCCAAACTCTTTCTCATGCTGATTAAACCAATTGCATATTGCACTGTATCAATAAACTGTTTGAAAACTGTTTTTTGGATTAGAACTGAAAAACTGTTATTATGCTTACCTCTATAATGATTTTGATTTAATAAGATGTTTTCTTTTAGTTCATTAACTAATTTTTCATGAACATATAAATTGTTTGATTGTTTAACAAAATTTTTAATAAAAGATAAACGTGCTTTGATCAAAAATTCATGAACCTCTTTTTTCGTTAGAATGAAACGTGCAGACATTAGTTTAAGTTCTTGATTAATTGTCTGGTACTTTCAGTTCAAATTAGGACCTGATTTTAAGCTGATAATTTCTTGATAAAGATTTTGAATATTAGCAAAGATTTTATTGACTAAATTAATACTTTCATCAATAGTTTCTACAAGTAATTTTGGAGAAAGTAAATCCATCTTCTTAATTTCTTTCAAAAGCAAAATTCTTGTTTTAATCCGCTTAGATACAATCTTTTTTTCTAAAATTCTTTTTCTATAAAAATTAAAAAAACTTCTTCACTTAATCTTTCAACCACTAAAAATTAAAGAAAAGGTAAATGAATTAATTAATTGCAAAGCATTTTTATGTTGTTTTGAGAATGTTTTCTTAGCATCAAAAAGTCTTTGTTTAGCTCTTTCAATTTGCAATTTTTCAAAATAACTGTTATCTCGTGATTTAGCTTCATAAGCAGCTATTTTCAGTTTGTTTTTTAAGTTATTTTTTTGTTCGTTCAATGCATAGTAACGCTCAAAAAAACTTTTTATTACTTTGTTATTAAAAGTAAATAATTGATTGAAAAACATGCAATTAAAATCAACAAGTTCTCTTTCGTTTTTTTCAATTAAAGTTAAAAAATTATTGTATTCAAAATCAACTTGTTCAAAGTAGATTGAATTAATAAATTTTGATGTAAAAAACTGATTAATGACTTTACTAGTTTTTTGATTAACATTTTCAATTTTCAAAATTGAAAACCTAAATAAATCATTAAGCGCATTAAAACCTAAAAATCACTTTAACTGTTTAGGTATATTCACTAAATTAAAGTAATTTTGATCTTTTTTATAACTTAAATCTTGTTTTAGAAAAATGCTAAATGAATTAGGAAATAACCACTTATTTTTATTTCTTAAATCTATCTCTTCACATAAATTAAACTTAATTTTTTGAAGCGGTAAATATTGATGGTTATTAACAAGCAAAAACCCAGAATAGGGTTGTTTATTTAACTGTAAAACTTGTGAAAGGGTTTTTCATTGTGCTTGATTTAAATTATCAATGAGATAGTTACCATACTTAAATAATGATAGCTTTGCAACCCCAAAATAGTTAGCATCTAAAAAGGAGGTACGAATCATTAATAATGACAAATCAACCCACTGTTCTAGATTATCAAAAAGAGATATTTTCTTTAGCATGTACCTTTCAAATTTTAATTGCTAATCTTTAAGGTACTTAAATAGAAATAATAGTTGAAAGAATTAAGGGTGCTTTTGATGTCATCTTGGTGAATAAATTCATTACTGCTCGTTTAATAGAAACCTTCAATTCTTTTGAAATTTCTTTACCTATTCGTTTTTTAATCGTGGTGAAATCCTTAATATCATTAATTTGTTTTTTAATTAATTCTTCCATTTTTGTTTTTAGTTTATCTTTTTCTGGTACATCTAAAGATACTCCTAAAAAAGAATAAGTTATCTCATTTAAGAATTCACTCTTTTTTTGATCAAAATAAATGATTATGATCACAACTCCAGATTCTGACATTTGATCACGTTCAAACATAATGCTAGCTCCAATTTCTTGTAATCCAGCAGAATCAACACACTTAGGATTTAATTTCAATTCATTTTTTTTGCTATCTAGTTTTTTGTTTTCAATAGTTAAAACTTCACCGTTAAAAGGAATTAAGATTTGATTTTGCTCAGCGCCAGCTTGTTTTAAAACCATTGTGAAGTTTATAAAATCCCTATAAAGTCCACCAGTTGGAATAATATATTTTGGTTTTAAAGAACTAACTAAAAACTTCATATCTTCATCACTTGCTTGGATAGAAAGGATTTCTCTACCTAAGTTGTAATAACTAACTTCATTACGTGCAATATCATCAAGGATTTGTGCTTCTATCTCTTCATAGCCAGCAACTTTAGGAGTCATAAAGATAAAAGTATCACTCTTACGATAACGAATTCTTTCATCTTCGTTCATTCCAATTTTGAATAATTTAGCATACAACTTATCAGGTGGTGAGGTGAGAACAACAATACTGTTGGTTGAATTGTTAATCTCCTCAATAGAAATGGTATTTAAATGCGTGTTATTAAACAATTTTTGACGCACAATTGTGTTAAAGAGATGCACAAATGATTGTGAATAGATAATAAAAGGACGATTTTGCATCCTTGCAATCTGGGCAAGAGTCATAACACTGTAAGCATTTGAATCATAACAAGCTACAAATATTCTGCCTTTGGCTGGAGTTATGATCCTGTTTAACTGTTCTAATGACTTGTGTTTAGGTGTTGTAAAACCACTGTTTCTACCAACTAAACCAACTCCTGTTATTAATAACAATGTATTGTCAGAAAGTTTAGGGATAATTTGATTTAGTTGATTTTCAAAAGCAATGTTCTTATCATTTAAAACAATGAAATCATCAATAAAAACGATGTAACCATTATCAGTATTTAAAGCAAATCCAAATGATGATGGTAAAGAACTTGATACTTTAAAAGGGGTAATAGAATGATTAGAAATTTCGATTGTTTCAAGTGGTTTTAATTCATGAATTTCCAATTTATCACGTGCAATATTAAGTTTGTTTTCATTAATTTTGCTTTTAATTATAGAAGCGCCTATTGAACTTGTGTAAATAGGAAAAAATCCTACTGTATGGAACAAAAATTCTAATGATCCTAAGTTTTCAGTAATGGCATTACCTATAAAAATACCTTTAACTCTTGCTTGATTTTCTTGAATTCAACTAAAATCAGGAATAATTTTTTTAACACCTAAAACTGCTGTTGTTGGTGTTAAGCTACCAACATTAAAGATAAACACATCATTGTCAATTTCAAGCACATAGCAATTTTTACCACGTTCATCTTGACCGCCGAGTGCAAAGAATTTAATCTTAGCCATTTTTAACCACGGAGGTTTAAATTTTTAATCAAATCACGGTAGGTTTCAATATTACGTTCTTTTAGATATTTAAGTAGCCGTTTTCTTTTTGATACCTTTGTATATAAACCACGCTTAGAAATAAAATCCTTTTTGTTTGCTAACAGGTGGTCTGTTAATTTTTTAATCTGATCTGTTAATATAGAGATTTGTACTTGCACACTGCCAACATCGTTTTTGTGAAGTTGATGAGCCTTAATGATTTGTTCTTTATCAATTTTCATTAATTAATAAACACGTTGAATTATAACAAAATTAGAAAACGGCTCATTGCCAAGGTTAGAAGTAATAAATTCTCCTACTTACTTATTACTTATAATAAATATAGTTTTTATTTTTATTAATAAATTCTCTCGATATTTTCGATATTTAAATCTATACATCTAAACAAATTAACAAAGCCATTTAACTTATGGACTCAACCTTTCATGAGCTTGGGATCTCTCAAACTTTAATTGAAACGCTTAATGCGCTTCATATTAATAAGCCAACAAAAATTCAACAAATCTCTATCCCTCAGTTTTTATCAGAAAAAAACTTAATAGTTCACTCGCCAACAGGAACTGGTAAAACTGCTGCTTTTGCAATTCCCATAATTGAGAAGCTATTAAAAGAAGATCAAACAGCAAAACCAACTTTAGTAATTGCTCCAACAAGAGAATTAGTAGAACAGATTAAAACCACATTTTCAAATATTGCTAAAAATAAAAAACTAAGAATTATTAGTTTAATTGGTGGTGTACCTGCTTGAAAACAAATCAAAAAAATCAAAACAAATCCCCAGATAATAGTTGGTACTATGGGTAGAATTATGGATCTTTTAGAGCGTAAAGCAATTCATTTTAACGATTTAGAACACCTAATTATTGATGAAGTTGATCTAATGTTAGACCGTGGTTTTAAAAAACAAATTTTTAATTTACTAGAACAAATCAATTCCTTTAAACAAATTGCTGTTTATTCAGCTAGTTACAACCAAGAAGCTATTAACATTGCCAAGCAAATTACTAATAATGGGATCTTTATTGGATCACCTGAATTTAATAAAGACGTAAATACCAATAATGATAAACTAATCAAACAATTTGTTTGTTATCTATTTTCAGATCAAAAAAAGCAAGCTTTATACAGCCTTATAAAAACAGCACAAGTTAAGTCAATCATTGTTTTTTGTGACACTAAAAAACTAGTTGATGATCTTCATGTATTTTTAAGAAAAAATGAATTAAGAACTTTTGCACTTCATGGTGATAAAAAACAATTTATTAGAGAGAGAAATCTTAAAATCTTTGCCAATACAAAACAACCCACTATTCTAGTAACTACTGATCTTATTGGTCGTGGTATCCATGTTGAAGCAATCGATATGGTTATCAATTATTCAGCTTGTTTAAATCTAGAAGCTTATATAAATAGAATGGGAAGGACTGGCAGAAACAATCATAAAGGGACATGTGTAACTTTCTGCACCTCACAAGAAAAGAAAGTCTTTCTGAAAATGGTTGAGAAAATCACTGATAATCGAATAGCTGAATGTAAACAAATGGAAATAAAGTTAATTCCTTTAAAAAATAAAGCTAAAACTAAAAAAGGTGGTATTTCACTTGATTGTGTTCAGAAAATATATGCCAATGCAAAACCATATGACCGTAATAAACGTGTCCCTTTAGCAAGTGATCTTTTCAAAAGTCGTATGCGCCAGCCTGAAAAAGCTATGCAAAAGCAAAAAATTCATGACAATGACTGACAAAGTAATATGTAATAACAATTTATAAAATACTTGTATGGCAAAAAAAGACCAACTTACCTTAAGAGGGCCTTTGTATGGCAATAATCGTTCTCATTCCAAAACTATTACAAGAAGAAAATGGAATGTAAACCTTCAGTCATGCAAAATTAAAGATACTAATGGTAAAGTAACACGGATTCTAGTTTCAACTAAAACAATTCGTACCCTTAAAAAACAAAATCGTTTCTAATTTAAATTTAAGTTAAACTTAATCCATAAGCATATATGGATAAAAAATACGATATCACAGCTGTTTTGAACGATGATAGTTCTATTAATGCAGTAAGCGATAACTTCCAAATAACACTGGATGCACGACCAAAAGAAAAATCTAAAGGCATTAATCCTCTGTCAGCTTTTTTGGCTGGTTTAGCTGCATGTGAACTTGCAACTGCTAATGCGATGGCAGCTGCTAAGATGATAACTTTGAACAAGGCACTGATTAACATCAAAGGTTATCGTTTAACAAATCCTAGTGATGGTTATTTTGGCCTACGTGAACTTAACATTCACTGAGAAATTCACTCTCCTAATGAGGAAGAAGAGATTAAAGAATTCATTGATTTTGTAAGTAAACGTTGTCCTGCTCATAACACTTTGCATGGAACTAGCAATTTTAAGATAAATATTAGCGTTACTTTAGTCCACTAAAACTTATTAAAAAAGCGAAAAATACCTGTAATTTTGCAGGTATTTTTTTATTTAAAAAAAGGATAAATTTAAGAAATTAAATTTCGCTTTAGCAGTCGATAATTGATCATATGAAAAATAATATTAGTGATGTAAAGTTGGGACTGTTAGCAGCAAAAATTTATTGAAAATCTTGACGCTTTTTAGAGTTAACAGAAGATGACATTATCTCTATTGCACTTCATGCAGAGCAAGATTCTAAGAAGCGTTTTAATCCTGAATTTGGCTTAAGTTTTGACAACTATCTCAAATTAAATGGAGCAAATTTCATTAGATCAAGTTTTAGAAGTATGGTGAATAAAGTTGAATTGCTTGATTCTAAAAGTAAATACTCATTAGAAAAGCAAAACACAGTTCTAAATACACCTGAAAACTATTTACGGAGTTTAGAATTTAAAGAAATTATTACTAAAGCTTTTAATAAAGCTAAAAACGATCAAGAGAGAAAAGTTTTTTCTTTATATGTAAAGGGCTATAAAAACTTTGAGATAGCAAAAAAGCTCAATATTAGTCCTAGAAGAGTGAGATATTTATTAGATCTTTTTAAAAGCTACATCAAATTGCTAACAGAAAGATATGGATATTAAATAAAGATATTTTTCTTTTTAAAGAAATTTTCTACTAGTTTTCTAACAGCACTATCACTAGTTAATTTCAATGCTTTTTCAACTAATGATTTACATTCATTAATTGTTATTTTAGCAATAACCATTCTTGCTTTAAACATTGAACTTGCACTCATTGATAACTCTGTTAACCCTAAACCTAAAAGCAACGGTATTGCATATTGATCACTGGCCATTTCACCACACATTCCAGTTCAAACATTATTTAATTTACCACCTTCTACAACTAGTTTAATTAAGCGTAGTAATGCTGGATTTAATGGTTGGTATAGATAACTAACGTTTTTATTCATCCTATCAGCAGCAAAACTATATTGGATTAAATCATTGCTACCTATTGAGAAAAAATCAACATGTTTTCCTAAGCAATCAGCTGCTAATGCTGCTGATGGAATTTCAATCATTATTCCTAATTTAAATTTCTTAGTTTCATTAAATTCTTGTTGAACTTTTGTTAAAAGTTGCTTAACTTGAACTAATTCATCAAGAGTTGCAACCATTGGAAACATAATTCCTAAATTTCCATAATCAGAAGCTCTTAATAAAGCACGAAGTTGGGTCTTAAAAACAGCTTGTTTATCTAATGTTAAACGGATAGCACGATAACCTAAGAAAGGGTTATCTTCATGAGGAAATTGAAAATAATTTAGTTTTTTATCTCCACCAATGTCTAATGTTCTAATTATTACCAAATCATTTTTAGCTTTTTGTAAAACAGTTTTATAAGCTTCAAATTGAACTGATTCATCAGGTCAATCTTGACTACTCATATAGAGAAATTCAGTTCTAAAAAGACCAATTCCATTCGTGTTATACTCAACTGCTAAATCCATATCTTTTACATTACCAATATTAGAAGCTACAATAACTTCATATCCATCTAAGGTTTTTACTAATTTATTGGTATATTGTTTTAATTCATTTTGAAAGTCGGATTCCAACTCTTTTTCTTGTTTTCATTGAGTTATGTCTTTACTTGAAAAGTCAAAACCCACAATACCTTTTCTACCATTGATACCAACTGTTTTGCCATCTTCAACTTTACTGGTAATATTTTTTAAACCAACTATGGCAGGAATTTCCATAGAACGAGCCATAATGGCAGCATGACTTGTTTTACCACCACTCTCAGTTAAAAAACCCTTGACATACTTTTTATTAAGAGTTGCTGTTTGACTTGGTGTTAAATCATTAGCTACTATAATGACATCACTTTTAATCCTGATTAAATCATTAAGTTTTACTCCAGTTAAATAACTTAAGAGCCTTTGGTGTAAATCTAATATATCACTGGCACGTTCTTTAAAATACTTATCATCCATTTCACTAAACATTAAAGTTGTTTGTTGAAAAACATTATCAACAGCAATAACAGGATGGATGTTTTTATTTAGTTGTTGTTCTAATTGCTCAGTGATAGTAGGATCATTAAGAATTTGGATGTGTGCATCAAAAATCATTCCTGCTTCTTGATTAATATTCTTAACAGTAATTGTCTTAATCTCTTCAAGGTCTTTTTTTGCTTTTTGAAAAGCACTGCTTAAAAGCTTTTTTGCTTGGGTGGGTGTCATTTTGACATTTGTGTATTTTTTAACGTCAAATTGAGGTGTTTGAATAATGAAAGCTTTTGCAACAGCAATGCCATCTGAAACACCAATCCCAATTATTTTTTTCATAGGTTAATTGTTATTTTTTAATAAGGATTTTGCAGTCATCTCTTTTGGTTTGCTAAGGTTAAGATATTCCAAGATAGTAGGAGCAATATTAGCTAAAATTCCAGTTTGATTAAAGTTAACATTTTTGTCAGTACATACAAATGGTACAGGATTAATAGTGTGTTTAGTAACTGGATTGTTATTATTATCAATCATCACTTCTGCATTCCCATGATCTGCAGTTAAAAACATAGTTATTTGATTAGCTTTACAAAAATCAACTATTCGTTTAATTTGAACATCGAGTGCTTCAAGAGCTTTAATGCAAGCTTGATAGTTACCAGTATGACCTACCATATCAGGATTAGCAAAATTTAAAACAGTAAAATCAAAGTTATTAAGCTTTTCTAGTAGTGCATCAGTAATAGCTTTACATGACATTTCGGGAGCTAAATCATATGTAGCAACTTTTAAAGAAGGAATTAATGTCTTTGTTTCATTGCTGAGATTAACTTCAAAACCACCATCAAAAAAGAAAGTAACGTGAGCATACTTTTCAGTTTCTGCAATCCTCAATTGCTTCAAATTATTATTAGCAATTACTTCACCAAGACTATTTTTAATGGTTTGAGGTGGAAAAGCAAATTCGCTAGGTACAATTCCCTCATAATTCATCATTGTTACAAAAAATAAATTTTCTTTTCGTTTCAATTCAGGTTGATAGTTGTAATAATTGCTGTTAAAGATCAAATGGGACATTTGTCTTGCTCTATCAGGTCTAAAATTAAAGAAAATAACTCCATCATTATTGTTTAATGCAAACTGATCAGAATTTAAATTGGCATTAATTGCAGGATAAATAAATTCATCAGTAATTTGGTTCTGATATTGCGTTTCAATATAACCAATTGGGTCATTGAATTTATTTTTTGAAACTCCTAATAAAGCTTTATAAGCAATCATTTCACGATCCCAGCGTTGATCACGATCCATTCCATAGTATCTTCCCCCAATAGTTCCAATAACAACATTAGGATAGTTTTTTAGAAATATCATTAATTTCTCAAGATCTTGTTTTAAGCTACAAGGTGCTACATCTCTACCATCACCAAATAAATGTAATACTACCTTTGCATGTTTTGAAAACAATTCAATGAGTGCTAATAGATGTTCATTATGACTATGCACTCCTCCATTGGAAAATAACCCAATTAAATGGATTTTTGAATGGTTTTTTTCTACATGTTCTATGGTTTTTAAAAAAGCTTTATTTGCAAAAAAACTACGATCCTTAATATGTTGATTAATCAAAGAAAGTCCAGTATAAACAACTCGACCTGCCCCAATATTTAGATGACCTACCTCAGAGTTACCTATTTGACCCATAGGCAATCCAACTGCTTCCCCAGATGCATCTAAAAGTACACAAGGATATGAATTGATTAATTCATCTAGCATTGGGGTATTTGCATTTTGTACTGCATTACCATAAATAGCATTTGAGATCCCATAACCATCAAGGATTGCTAATAAAACTTTTTTATGCATATACCCTAGCCATTTCTAAAAAGTTTTTAATTTCTAAAGATGCTTTACCAACTAAAAATCCATCAATTTGTTCCATTATTGCTAGTTTTTGGATATTATTATGATCAACTGATCCGCCATATAGAATTGAGATATTGTTAGCAACATTTTCATCATATAAGTCATTAATATATTCCCTAATGGTTTTAATGGTTTGATTTGCAACTTCAGGAGTTGCTGTTTTACCTGTCCCAATTGCTCACAAAGGTTCATAAGCAATAACTAAATTTTTAATTAAGCTTTTGTCAATCGTATTTAAGCAATTAGTAAGATCAGTTTTAAGAAAGCTAATCTCTTGTCCTAAAGTCTCACCAATACATAAAACTACTTGCATGGATGCTTTTAGACAAGCAAAGAGCTTTTGATTAATAACTGCACTGGTTTCGTTATAGTATTTTCTTCTTTCAGAATGACCAATAATACTGTTGTTAACACCAATGTCTTGAAGTTGAGTAAAGCTTACAGTTCCAGTATATGAACCACTTTCAATAAAGTTAGCGTCTTGGGCAAATAAAAGGAGACTATCACTAATTATTTTTTTTATTTCAGTGAGATGAACATAAACAGGTGCTATCCCAATTTTGGCATTGTAATTAAGTTTATTTTGTTGAAATTGTTCAACAAAACTAACTGCGTCTTTTAAATTTTTATTTGTTTTCCAATTGCCAATTAAATACCTTGTGCGCATATGAAAGATTTTATAAGTTTTCAAAAACCATTTTCTTTTCCAACTAATTGATCATTAAGTTATGACTTGTTTTCAGTTCCATGTTTTGTTTGCTTATTGGCACGTCTATCATAGATATAGCCATCAAGCTTTTTAATAAAAGTAATGGAAAACAAAGCATCACGATCAAACTTTCTCACATCTTCTAAAAGCTTAGCAGCTTTAATAAGCAAACAATTAGTTACAAGCACTTGGGTTTTTTGGCGTGAATATCCTCCTTCAGCTTCAAACATTGTGATTGAAAATCACTGTTGATTATCAAGTAGATAGTTACGAATTTTTTCTATGTGCTTACCATAAACTTCTACTTTTACAAATTGATACTTAGGGAAATAAAAAGAAGTAAATAATCCCAACACAATGTTCATCAATAGAGTAAAAACAAGGTTTGGAGATAAAAAGAAAGCAACTCCAAATGGCTGGTGTTTATCATCACCAACATAAGATTGCACTGATAAACTACCAGTTAAATAAGTACCAATTACATAGCCAATAATAAAGCTAACTGTATTAATTAGCATCAAAATTCCACCAATGTCTTTGTATTTTTTTTCAGAATATCAGAATGCTAAAAAGTCAAGACCGCCTGTAGAAGCGTCAATAATTAAAATAACTGAATAAAAAACTGCTTGTAAAAACCCCCAAATAAGTCCATAAAAAAGTAAAGATACTTGTTTTTCAGCAGATTTTTCTCAAAATATAAGTTGTACTCCCTTTTCATTAATTAAATTTTCAAATCCACCATCTTTTGCTGTAGTTAAATTTGCAAACAAAAAGAAGTTATCTATTCCAGGGATGTATGAAAAGAAAAAACCAAAAAGATTTGAAACAGCTACAAAATAAAGGGTTAATAGGGTAAATTTTTTGGAGATTTTAAACCATCCAAAAATAAAGAAAGGAACATTAAAAAGAATTTGTGTTAACCAAAAAATAGCATTAAATATAGTTGCTGAATCAACATTAATATTTTGTGAAGTAATAAAAAAATTAACAAGACGAGCTAATCCTTGGCTAATGGAAGCCATACCAATGTCATAAAGTCCTGAAAACTGAACAAAAATAACACCAAGTAATCCTCAAAAAAAAGCAACTATTGTCAAAATTACAAGTTGTAAATAAAGCTTTTTAAGGTTGTATATAGATTGAAACTTAAGAAATGATCCAGAAAGATGAATCCTTACAAAATTACCTGAAATTTTGATGTTTTTATCTTTCATAGTTAAGAACAATTATTTTAATAATTTTGGTAATTAATAATTTCTTATTTACTATCTTATTAGTAATATTAAGTTTAGTGCAATAATGGCAACGAAAATAGAGCTAATAAAAGAATTGCGTAAATCAACACAAGCAAGTGTTATGGATTGTAAACAAGCTTTGGAAAAAAATAATGATGATTTTGAGAAAGCTGTTAAGTGATTAAGAGAAAATGGCATTGTTAAATCAACCAAAAAATTAAATAAGGTTGCAAGTGAAGGAATTATTGTTTTAAAAAGCAATTTACACAAGGCAATTATGGTTGAGATAAACTCACAAACTGATTTTGTAGCCAAAAATCAAGAGTTAAAAGAATTTTCAGATTTAATGCTTGAAAAAATATTTGAAAAAGTAAATCCAAAAACAGAATTAGTTGAAATTGAAAAAATTCAAATTAATAATGATGAAAAAGTTAGTGAAAAACTAGCATTAATTGCTTCTAAAACTGATGAGAAAATAGTACTTAGAAGAGTAGTTGTATTTGAAACTAAAACTAATCAAATTTTCACCTATTTACATGCCAATAAAAGAATTGGGGTAATTATTGAGATTCAAGGAAAACTCAACGAAGATGATGGTAAGCATTTAGCAATGCATATTGCTGCTAATTCACCACAATTTATTGATCAAAGTGATGTTAATCAAACATGACTTCAAAATGAAAGAAATATTATCCGTTCCCAAGCAGAATTAGAGGTTAAAGAAAATCCTAAAAAAGCAATTTTTTTAGAAAAAACTATTGAAGGTAGAGTTAACAAATTACTAATTGATACCTGCTTAATTAACCAAAAATACTTAATTGATGAAACTAAAACAATTGGTCAATTTTTAAAAGAAAAACAAGCTAAGGTTCTTAAATTTATTAGGTATGAAGTGGGAGAGGGGATTATAAAGGAAACTGTTGATTTTGTTAGTGAAGTAAATGCACAAATCAAACAATAAAATCCGCCAAAGAATAATCATTAAACTTAGTGGTGCTGGGCTAACCAAAGAAAATTTTCAACCCTTTTCTAATGATTTTTTTGAAACTATTATTAATCAATTAAAAGTTTTAAAAGAAAGCTATCAAGTAGGAATTGTTATTGGTGGGGGTAACATTATCAGAGGTAATAATTGCCAAGAATTTAACATTGCTGAATACCATGGTCATCAACTTGGTATTATAGCAACAGTAGTTAATGGCTATTTTTTAAAAGCAAAGTTAGATGCACATAATTTGAAAAGTGCTTTACTAAGTGCAATTAGTTGTCCTAGTTTAGCAGTGCAAATTCTTTCACAGCAAACTATTGATAAAGCTTTTGAAGAGAATGACTTTGTCATTTTTTCAGGTGGCACTGGTAATCCTTATTTTTCCACTGACACTGCATTAGCTTTAAGAGCAGTGCAAACAAAAGCAGTTGCTATTCTGATTGGAAAAAATGGTGTTGATGGTGTTTATACAGCTGATCCTAAAAAAGATAAAAATGCAACCTTTTTACCAACACTCAACTATGACCATGCCATTAAAAATGATTTGAAAATTATGGATATTACTGCTTTTACTATGTGTAAGGAAAATAATCTGAAAATAATTATTTTTAACATTAATGCTGAGAATGCATTATTAGATGCATTAAACAAAAAAGGTCGCTTTACTATAATTGAAAATAACTAATGACAAAAGCACATTACATTGATTTTTTTAAACAAGCAGCTGATAAAAAAATTCAATGATTAAAAGAAGAGTTAACAAAGATTAGAACAGGTAGGCCAAATCCTAAAATCTTTGATAATCTTTTGATTGAAAGTTATGGACAAAAAATGCCTTTAATATCTTTAGCTCAAGTGACTATTAATCCGCCAAGAGAAATAATCATAAAACCATTTGATCCTAAGAGTAATACTAATGCTATTTACAGTGAAATTCAGCGGGCAAACATTGGTGTTCAACCAGTTATTGATGGTGAAAAAATTCGTGTTAATTTTCCCCAAATTACTCAAGAAACTCGCTTAGAAAATATTAAGCACGTTAAAAAAATAATAGAGCAAATTTATCAAGAACTGAGGGTTGTAAGAAGAGATGCATTACAAATGATTAAAAAAGATAATCACAATGAGGATTTAGAAAACTCTTTAAAAGCTGAAATAGAAAAAATTAACAAAAATTATTCTAATCAATTAGAAGAGATTCAAAAAGACAAAGAAAAAGAATTGCTAACAATTTAAATGAATGAAAAAGCAAAACAATTCATCAAAAAGCGAACTTCAGTATTCATTGCTTTATTAGTTGTATTTTGCTTTTTTCTTTTAATTAGCGCATTTGCTGATGGTTTTAACTTTTGATCACCGTGATCAGCAGATTTCAATTCAAGAACATTAAAAGTAGAACAAGCAAGTGGTGTTACTAGTGTTATTAGTACTGAGATTAATGAAAACTTTAAAGCTGTTCGTTTCAGCTTTAGCATAATCATTATTTTAATTGTTGGGGTAATTGGTTCTCTGATGATTTGAGAGTTGTTCACAAACATACTAAAAAATAAACCAAAACTAAGCTTAAGTTTAACGTTGTTAAATGCTGGAATAATTATTTTTGGGATGATTGGTACTTTTGTTGTTGTTTATTTTTACAAATGAAATGCAACTGTTAATGGTATTTGAACATTAAGTTTTACTCTTTCTGTGGTTTTACTTTGAATAATTTACATTGCTTGCATGAGTAAAACAAGAATTAAGTTTAGCTTACAACTTTCATATAGCTTAGGAGCTATTGCTTGCTTTATTGCTAGCATAGGTACTATTTACTTTTCTGTTATCAGGGGTTGAACTACAATCTTTTTATTGATGAGTTTAGCAGTCAGTGTTGATACATTTTCTTTTCTTTTTGGAAAGCGCTTTGGTAAAAATCCTTTAATTAAAATTTCACCATCAAAAACATGAGAAGGAGCTTTTTTTGGCATCATTAGCACCATTGTTGTTGTTGCTTTACTTTGTGTTTTATATTCAATTCCTTTCTTTGTAGCAAAGCCTACTTTTAATCAAACAAATGGAATAGCGCTCAATACACCCCAAAATTATGATAGCCATAATCTTATTACCAATATTTTTTTAATTGCCTTTATCTCTGGAGGAAGTAGTTTTTATATCTACTGGTGGGTAAGCACTTTAGCTTTAATTTTTACAGGATCTGTTTTTGCAATAGGCGGTGATCTTTTTTTTAGTTATATTAAACGCTTAATTAGTATCAAAGATTTTTCTAAGGTTTTAGGTAAACATGGGGGAGTTTTAGATCGATTTGATTCAAGTTCTTTTTTAATTAGTTTCTTCTTTGTTTATCATTTAATAGCAGGAACCATTTCCAACCAAAGGTTGTTGATGGAACCTAATACTTATTTCAGTGCAATCACTAGTATTCAAAGCTAGTATTTAGAATTAATAAAGTATGACTCCAAAACTAAAGCTAAATAACAACATAAACTGAACCAAAAGAACAATTGATTCTTTGTTTGATTTAAAAAAAGGTGAAATGCTTGAAAAAGAGTTAATTACACCTGAGGGAAAATATGAATATTTTAACGGTGGTGTAAAAAATTCAGGAAGAACTGACAAGTTCAATACTTTTAAGAACACTATTAGTGTAATTGTTGGTGGATCTTGTGGTTATGTAAGGCTAGCTGATAAAAATTTCTTTTGTGGTCAAAGTAATTGCACATTAAATTTGTTAGATCCGCTTGAACTTGATCTTAAATTTGCTTACTATGCTTTGAAATCACAACAAGAAAGAATAGAGGCTTTAGCTTTTGGGACCACGATTCAAAACATTAGAATTTCAGATTTAAAAGAATTGGAAATTCCTTTCACATCAAATAAGAATGAACAGCATGCTATTGCAAATACTTTAAGTGTTTTTGATGAGAGACTAGAAAACTTAGCTTCTTTAATTGAGATTAACAGGAAACTAAGAGATGAATATGCTCACAAACTCTTTAGCTTAGATGAAGCTTTTCTAAGTCATTGAAAACTAGAAGCATTACAAAGCCAAATGCATGAAATTACTTTAGGGGAAATATTTAATTTCAAAAGTGGTAAATATCTAAAGAGCGAGGAGAGATTAGAAGAAGGGAAATTTCCTTATTATGGAGCGGGAATTGATAACACTGGTTTTGTAGCTGAACCTAATACTGAAAAAGACACAATTTCTATTATTTCTAATGGTTATTCACTAGGTAACATCAGGTATCATGAAATTCCTTGGTTTAATGGCACAGGTAGCATTGCATTAGAACCCATGAATAATGAAATCTATGTACCATTCTTTTACTGTGCTTTAAAGTATTTGCAAAAAGATATTAAAGAAAGAATGAAAAGTGATGATTCACCTTTTTTATCCTTAAAACTAGCAGGTGAAATTAAAGTGCCTTATGTTAAGTCATTTCAACTGCAAAGAAAGGCAGGAAAAATCGTCTTTTTGTTAGATCAAAAATTAGACCAATATAAAAAAGAACTAAGTTCTTTAACAGTGATTCGTGACACTTTGTTAAAAAAATTATTCCCTGATATGACTGAAAGAACTAAATCTATTAAGGATTATTAATCAAAAACTTAATTCTTTCTTTCTAAAATTACTTTAATATCTCTTTTCTTGCAAAATTAGATAATCTTTTCACACCAATTGAATAACCTGATTCAAAATATCCACCATTATTTTGATAGATAAAATTAACAAATACAAAAATTTACTTTCTTTATTTTTAATTTATCGATAAACAGTTCTTACAGCGCCAATATTAGTAACACTTTTTTTGGTTTAGCATTTACTTAATTTTTAGTTTCTTTAACAAATTGAATTGTTATAAAAATGCGATTATTTTCTTGGATTTTAACTGCTTAACTTTTTTTATTTTGATATAAAGTTAACTAATAGAAAAGTATTTATCTTCTTTAGTTTTAAGATCAAAATATATCAGGTTTTATTTTTAAAAAAATTAAAAAATCTTATTAAAGCAATGTGTAATAAAAAAACGGTAAAAGATCGATTGACTTTCCCTTAAAAGTTCTTGTTCTAATTTTCTTCACTTGTTCATTTGTTGAAAGTAATAAATTCAACTTTTCATGAGAAGTACTCATGTCAATTTTGATATCAAAAGGAAAGTTAATTGAAGCAGGTATACTACTAAAAGTACTTTCACTTACCCTCCTATTATCTGTTGGAGGAGTATAAGTAAAGATAACATCACCATCAAAATTAGCTTTATCACCAGTAAAATCACTTTCACTTACACCACTTGTACTAGCTAAAAAGGATTTGATTAATGAAGTACTAGTATTGTCACTACCAACTATACCAGTCATTTTTAAGTGAATTTGAACTTTAAAATCTAAGTTATTTTTTCAACTGTATTGAAAATTAACTACCTTTTGACTTTTAACTCCTAAAACTAAGGCGATTTGACCACTTATACCTGGATAAGTTTCATCAAAATATAAACCTTTTGCACTGATGTTAGGAGTAACATCTTCCTTTGATTTATTAACTCCAAATGCTTTAGCAAATCTGCTATAATCAAGTGGTTTTTCTAATAGTTGATAATCTAATAAAGTTGTTTTTCAACTGGCAAATACAGTATTAATTACCTCTTCCTTATCAGAACTATAACCCTTTTTTAAAAAAGTAACAGCATTATTTTTTGAAAGTTCTATATTGTTTCTCTGAAAATAAGCTTGTGCTTTTTCTAAACCAGGATCAGTTGATGAACAGGCTACAGATAAAGTACTAAGTGAAATTAATGGTAATAAAAAGATCTTTCTGAGTTTCATTACAAAATGGAATAAAGCACATATGGTAAAAGATCGATAGTTTGATCTTGGAAGTTTTGATCAGCTAATTGACCTACAAAATTACTTTGTAATAACTTTTGCATCAATTCATCTTTACCAATAATTTGTAACCTTAAATTGGTATTAAAATTAATTTTTCTCAATAGAGTATCAAAACTAGATTTGGAAAATAAATTACTGGAAGGTGGAGTATAAGTAAAAATTAAATCAGCTGAAATAGAATTTTTATCTTTTATATCACTATCACTTAATCCTATTTGACTCAAATATGATTTTGCTTGAGCACTGTCAAAAGTTCCAGTAGCTTGTAACCTTATTTGGACTTTGAAATCACGAGAACTGTTATAGTAAAAACTAAAATCTGTAACTGTTTGACTACTCAATTTAATTACATTATTAATAATACTTGCAACATTTTGCGTGTATCTTTCAACTAAACGTAAGCCTTTAACACCAGTGCTTGGTTCAACATCTTCCTTACTCTTACCACTGCCAAATGCTTTTACAAATCTACTTGGGTCTTGTTTTTCAAGAATCTTTTCGTCTTGCAAACTAAATCTTCAAGCATCTAATAGAACGTTTGTAGCACTTTTTGAATCCACTTCAAAACTATCTCTTAGTGAAGTAACTAACTCTTTCTTGTCTTGATTTAATTCACTATTTTTACTGAAACTATTTCTAAGTTGGTTTAAACTGGAAAAATTTGTTCCACTGCTTAAGTTAGAGCATGCTGTAATTAAAGAACCTAATACCATTAAAGAAGAAATGATGCCAGCTTTTGTTCAAAATTGTTTTTTTAACATTGTTTAAGTTAACAGATTTTCTTATTTGTAATTTTTTAATCATTAATAGAGGTAAACTTCAACAACAATCATTTAGAAGTTTTGTTTCTATTTTTATCAATTAACAAATAGAAGGTTTCTATACTTAAAAAAACAAAAATAATCACTTAATTTTGAATTAATAACTTCTTGTTCTTGCAAGCCTAGATTATTGACATGAAACAGTTTTACTGCTTGAAAAGTTTCATTGGAAATCGATTCTCTACTTAATCTAATTCCAAGATTTTGAATTTGTGTTTTTATAAAAAAATGAATATTAGCAATTAAGTTTCAAACTATTTCTGTTTTTTGAAAAAGTCTAACTAAAAGATATAAAGAAGTCTGATTAAACAAAAAATGCTTAAATCTTTTTCCAAAGTTTCTAAATAAAACTAATATTATTTTTAAACAAAAAAGAAACAGTACAAAAAATGCAAATACTGATGCTTTTAAACTAAATTTAGATCTAAGAAAACTAACACTCATTGCGTTTTAAGCAAACTAAAAACAACAATTAAGAATGATTTTTCAAAAGTTGCAACTATAATTTTAAAACTTTTATTGATTTCTTTTGTATTAAAAACATTTGCACAACTTACTCTAAATTAATATCTTTTTACTAATAATAAGAGATTTTTTTAAAACTAATGGAATTACTAATTAAGTTCATAACTTAATTTGCCATATTTCCCATTAATAAGTTCATTAATAAAAATTTCACATGCTAAGTTAGTGTTTAGTTCATTAGCTTTCTTAATTAAGCCTCTCACTTTAGCAAATTTTTCCAAAAAGTTAATAAAGCTATCCGCTTCAAATGGTAAAAGCTGTTTGTAATGTTTCTTAAGATAATTAAAAGCAAACATTCCCACTTCTTCTATATTAACTACTTCTCTTCTAATAACATTAGTCAAAACTAGTTTATATCCAATTTGAATTTCATCAATTCTTTTCAAAAAAATTCCTGGGGTATCACTTAATAATAATTCAGGGCTAATTTGAATCCAATTTAATGATTTAGTAATACCAGCGCGGTTAGCTACTTTTAAGTGATTTTTATTTATCAAAAGGTTAATTAAACTAGATTTACCTACATTGGGCATGCCAATAACTGCTAATCTAAATTGTTTGATTAATAAGCCTTTTGCTTTTAATTGTTGTCTTTTATTTGCAAATAAAGTTGTTAATGTCTTTAGAACTTTTTTTTTGAGTTTAAAAGGTTCTTTTAATGAACCAAATAAGATCTTTTTATTTGGTTTGTATTGTGCTAAATCTGTCTTTAATGCCAGTATTAATTTTGGTTTATTTAAAAAATAACTAATTATTTCTGAATTATGAGTTAAAGTTGGTGCTCTAGCATCTACTATCTCAATGATGCCATCAATTTGACTAGATAACTTTTTTAATTGATCATGGGCCTTTTTCATGTGACCCGGGAACCAATTAATTTTGGCGGAGGTATATGTGTCCATCTATTCCCCTGATTCTTGTAATAGATACCAAACAATCCTTGTCAATTTGTCTAATAATCTTAATTAAATGGGGAATTTCTACATACATGGAAACAGAAACTATCATCTTCTTTTTGAGTAAAGAATAACCACCAAGCGTTTCTTGAATAGATAAACTGTGATTAGCATTATCACTTAACAATGCTTTTCTAACTTCTTCAAGCTTATCAGTAAATACTTTAATTTCAGCAAAATTATAACGAGGGAAAAGATAACTAACTACTGTTCCTGTTAACAAAATTGAAAAAAAAGTTGCAATTAAATTAGGTGAGAAAAATAAACTAACTTCCCAAGCAGAATCACGATAATTATTGACATCTTGCAACAATAAACTACCAGCAACAAAAGAACCAATTAGAATAGCAATAATCAGAATAAAACTATTTACATAAAAAAGAATTGATCCTACTGATCTATTTTTCTTACGAGCATAATATTGGGTTAAGAAGTCCGCTCCCCCAGCAGAACCACCTAGTATGTAAAGTAATGAAACTGATATGCCATTATAAAAACCATAAATAGCTGCATAAATAAATGTGGAAATAATTACATTACCTTGGGAAGTATCACTTCATAAAAAAGGGACAAAACCGGCACTTTGATTTAAATCTTTCGCTGCTTTTCAAAAGTTAGTATCTGTTATAGAAGCTAACATTGGTGGTAAATTATCAGAACCTGGAATAATACTGATTAAAAAGCCAAATACGTTAGATGCAACAACAAAATGGGTTGAAAGAATAGTAAAGTTTTTACCTATTTTTTTATAAGAAAAAATAATTAAAGGGATGTTGATAAAGACATATAAAAGCCAGTAAAGGATGTTAAAAATTAATAATCTTTGAGTTTCATCAAAACTTTTTAACAAAACAAACACTAATCTAGCAAAACCTTGAGTTAAAGAACTAATTCCAAAGCTATATAAACCTGTTTTTTGAATAAAAATAAGTAAAAGAAAAGCATTAATAATTGATAGTAAGTAAACTAATGCATACTTTAATGGTTTTTTAGCCTCATAAAGATTGCTAAACATCAAAAAAGAGTTAGAAATCCTTACGCGTTTAGAACCAGATGTGACTGTTATCTTTGATTCTTTTTTAAATAAGTTGTTAAAAAATTTCATTTGTAACAAACTTTTTTAAGACTTTTTTTCTTTAATTTTTGCTGACTTACCAGATCTTTCACGCATATAAGAGATGTATGCTCTTCTTACTTTACCCCTGCGTTTTACTTCTATGTCTATATTAGGGTTGTGGATTTGAAAGTTTTTTTCAATAGGAATTCCATCAGTGGTTTTTCTTACCATGAAAGTTTCGCTAATCCCTCTTCCCCTTCTTCTTAAAACAGTTCCAGTGAAGTTTTGAACTCGAACTTTTTCTTTTTCACGTAACTTAATAGCAACATTAACTTCATCTCCTGCTCCAAATTCAGGAACATATTCCTTTAACTGTTTTTGTTCTACTGCATCAATTAATGCTTGTTTATTTATTTTTTTCATTTTTATTTTCTAAGTATTTTTTGTATAAATCAGGTCTGTATTTTGCAGTTTTTAAGATTTGCTGTTCTTTACGAAATGATTCAATCTTTTGGTGATCTCCTGAAAGTAAAACTTCAGGAACTTTATCGCCTTTTAAATCGTATGGCCTTGTATAAACAGGAAAATCTAATAGATTATCATTGAATGATTCACAAATAAGACTCTGATCATTAATAACTCCTTTAATTAATCTAACAGTAGCATCAATAACACTTAGTGCTACAAGTTCCCCACCACTTAAAACAAAATCACCTAAAGAAACAATTTGATTAATATATTTATAAATTCTTTGATCAAAACCTTCATAGTGACCAGATAACAAAATTAAGTGTTCGTATTTTGTAAGTTTTTTAGCACAATTCTGAGAAAATTGTTCACCTTCTGGGGAGAGTAAAACTACATGAGAATTTGGGGCTTTATAAAAATTTAAACAATTAATAATAGGTTCAGCTTTTAAAACCATGCCACTTCCTCCACCATAAGCCATATCATCCACAGTTTTATGTTTATCATTGCAAAAATTTCTTCAATTTACTACTTCAAATTGAACTAAATTTGCTTTTTGAGCTTGTAACATAATAGAACTATTTAAGTAAGGCCAAATAGTGTTTTCAAAAAGTGTTAAAACAGTGATTTTCACTACTTTTTAGGACTTGTTTCTTTCTTATTTTTACTTTCAATAAATTTCTTCCACAAACCAGATTCACTAAATAAAGAACGGACTGTATCAGTTGGAATTGCCCCTTTATTAAGTCAATCTAAGGCAACAGTTTCATCTAATTTACACTTATTTTCCTTTAAAGCTGGATTTAGATGTCCAATTAAAGCGATATACTTTCCATTACGCTTTACTCGCGAATCAACCGCTACTATTCTATAAAGCGGATAGTGTACTCTTCCCATCCGCATCAATCTTATTTTGACCAATGATTTTTTCTTAATAAAGCGGCTAATTATAACTTTTTAAAGTTCTATTTTATCTATGCTTAAGAATAACTTAATCAGCTATTTTTTATTCGTTTAGTTAATAAATTTAATCATCCAATAAGGCCCGATTTTCATTTTGCATGTCATCTGCAAACTCATTATTTTTTTAGATAAATAAGTTCTTATCTTTATATCTAACAAAGTCTTAATTATTTTCTGTTGTTTAGCATATTATTTTAAGGCGTTAAACTTGTTTTCAGAATTAATGATGAAATAGTTGTTTTGCTATTCACAATAGCAAAACTTAAATTTGAATTATGTTTTTGTTTATTAATCTGTTCAATATGTTCTTCTCAAATTAACCTATATTTTTATCGGTTAATTCTTTTTTAATTTCATATAAGTAGATTAATGTAGTTCCACTATTAACAAATTTTTGTTTATTTTTAAGGAATTCCAATAGTTTTTGTCACTTTCATTTGGGAGGAGAGATTTACCATTTTTAATTGAATTAATTACAAAATTTTTTTTAACAAATAATTAGTTGTTTAATAATCGAAATTATTAATTAGTTTTTTTAGCATTCATACTGTAATTGGATTTTTCTAATTATGAGTACTTTTCAATAAATTTGACCAACTTATTTTGTGGTGGATTAGCGAAATATTTATCCATAAACAGATTAGTGTGAATTTGACCAAAAGCAATATTAATTAAACCTGCTAAGGCAGCATTTGCAGTTAACAATCAAATAAAAACTTCAATGTTATTTGTTTGAAGTGTTATTGCTTGAAAAATAAAAAGTAAAGGAATAAATACAAACAATCCGTGTGTTAAAGAGACAATTCAAGAATAAAGCACTCTGTTAGTATTTTGAAAATATTGTTGACTATTAGCGCCTGTGGCTACAAAGAAAACTTGTGCTTGCACTATTAATGAAAAGTAATTTGCTAAATCCAAATTTTGATCTGAAACATCAAAAAAACTAGATAAGATCTGTTTTCCAAAAGCAACTGCTGTTAACAAATAGATTAAACTACCGAACGATATACAGATAATAACTGTATAAATGTTAATTTTTTTAATTTCATCATATTTTTTTTGTCCAAACTTATATGATGAAACTGTTCTAACTCCTTATAATAAACCAAAAATAGCTGCTGAAGCTAAATTACTAATAGCAATAGGTCCAGTTAAAAGTGTCAGATAAAAAACATCATTTTTATCAGTAGTTGCTTTTGTCAAATTAACCAAAAAACTCTCATAAAATGTAGTAACAATAGAAAGTGATCCATTTCTAAAAAAGGATGCTATTCCTATTAATGAAACTACAACTAGTAGATTAAAGTCAATTTTGTTTAATTTAATAGTTTTAAAAGTTAAATAAGTAAGATTTCTTTTGTTTAAATGAATTAGATAAATAATATAAGCTAAAAAATTAATTAAGTATCCTAGTATTCCAGCCACAGCTGATCCTATTACTCCTAAAGAAGAATATCTAACTAATAAAAAAACTATTAATATGTTAATCAAATTAGCAATAGGCGGAACAATTGCAATAAAAAGTTGTCTTCCCTCTGATTGTGCTAAATAAAAAAACAACCTTGAAAGCATAGGAATGATGTTAAGACCAATCAAAATATAAACATATTCAGATGCTACATCAATAGCCTTTTTGTTAAAAAACTGTTGGAAGCTATTAGCTTGAAAATTTTGTTCAAAACTTGATTGATCAAGATTATAATGCAACCATTCTTTTGCAAAAGATAAAACCAAAAATTGGGTAATTAGTCCAAATACAGTTGTTGATATTAATCCGGTATTTCAAGCTTCCTGAATTTTATTTTGATCATTTCTACCAATTGCTTTAGAAAAAATAACTCCAGTTCCTAGAGGAATAAAAATATTAATGGCGTTTAACAAAACAATCAAAGGTTGACTAATATTTAAAACAGTTTTAATAAATTGTTTAATATTAAGTCCAGAGCTTAAAAAACTATCACTTTTATAAAAAGCAGAATTTTTAAATTCATCAATTAAACTCTGATCAGTAAAAATAGAATCAGGATTAAGTTCACTTCTAGGAACAAACTTAATCACCATGATTTGATCTATAAATATATAAGCAGCACTAAAAAGTGCAAAGAAAAAAGTTGGAACAGTAAATCTAAGAATAGTTAGAAAAAGCTGTTTTGATTCAAAAACATTTTTAATTAGGAGTTGTTTTTCAAAACGATCCCTTTTACTGTTCACTTATTTATTTTTAAAAAGATGGATGTAATCCTTGTATCCTAATTTTTCCATCTCTTCAAAAGGAATAAATTTTAAAGCTGCAGAATTAATACAATAGCGCAATCCACCTAATTCACTCGGTCCATCATTAAAAACATGTCCCAAGTGGCTATCACTGTTTTTAGCACGAACTTCAGTTCTTATCATTCCGTGAGATTCATCACGGTAGTTAGCAATTAAATTTTTATCAATGGGTTTTGAAAAAGCAGGTCAACCACAACCAGATTTAAATTTATCAGTTGAAATAAAAAGTGGTTCACCAGATGTTATATCAACATAAATTCCCTTTTCAAAATTGCGGTTATATTCATTAATGTAAGGTGGCTCAGTGTGAGCATTTTGCGTTACATCAAACTGGAGTTTGGTTAAAGTACGTTTTAGCTCAGCTTCACTCTTTTTTTGGTATTTACTCATTTGGAATGTTGTTTATTTAAATATACATCAATAAATGGTTTTCCTACTTTGATCTTAGGATTAATCTCAATTATACCCTCATTTTTCTGTTTTAAATTAAGTTCTTTTTCAGAACATAACATGCCAACTGAATCAAAACCAGCTATTTTAGTTTTTTTGATAACTGTACCGCTAGGTAACACTCCACCTACTTGTACTAAAACTGTTTTCATACCAACTCTAACATTATTAGCTCCACAAACAATATCCAAGCTTTTTGTCAAACCAGTGTTAACTTTACACCTTTTTAGATGGGTGTTTGCAATAGGAATAACTGAAACAACTTCACAAACAACAAAAGGAACTTTATTTGCTAATGAAATAAGATCATAACCTAAAAGCTCACTTATTCTTTTCATTATTTTTAATGAAGGATAGTTTAGACCTTCATTAAAGCGGTGTGATAAAAAAAACTTCTTAAAACTTTTTTTGATATTAAAAAAGTTAAAACCAGTAATTCTATTTGCATCATTAACAAAAAAACTTCAGTCTTTGTCATTTTGTTGGCGTAAAGTTGTTTCACTTCTACTTCCTATGATGCCAAACATACAGTTTTTTAATGTTTCTTTACGATAAAAGATAGAAACAAAATCTTTAGATATATCAAACATAAAAGTTAGTTATTCTGTTTGTATTAAAAAGGCATAACTATTGATACCTGTGTGTGTACAAATTACAGGTGAAAGCAAGTGCTCACTTACATCAACTGCATTGTTGAGAAGCGTAACAAATTCTTGCTTTATGAGTTGATTTTTATCAGTTTCAAACATTGTTGTCAACAAAGCCGCTCTTCTGAAATTAACTTCTTTTTTTAATAGCTTTTTTTCTAAAAATTGAACTGCTAACTTATGAGCACTGCTAGCAGATTGTGCTTTGTTAAAAAACTGCAATTTGCCTGTTTCACCAAGGAAACTAATTAAGAGATGAAATTTAAAGCTTTTGATAATAAATGACTTTAAATTAGATAATCTTCCTCCAGCTACTAACGGTTTTGTATCAGTTACAAATAAAATTGCACCGCACTTATTACGGAAGTTATAAACAAATTCATCTAAGCCTGCTCTTGAATACTGGTTGTTTTTAAGTCAAACCTTAATATCACTAATCAATCAAGCTATTAAAACTTCAACTATTCTGCTATCTAAAACATGAAACTTGTCTTCAAATTCCTTTTCAACAATCTTTCAATTTAAATAGGAGGTTGAGATCTCTTTATCAATAGGGATTCCTATGATAAGATCATAATCATTAATTATGCTCTTAACTTTGTCACGAACCTCTTCTTCAGAAGTTTGCGATGTTGAAAACTTAACACCTTTAGGGGCGTTAATCATCTTATCAGTTAAGGTTTTAAGATCAATATTAAAACCTGATTTGAATGTTTCTTGGTGTTCAGCAATTGTTTCAATAATATAAAGCGATAAAACATAGAGATGACTATTTTTATCTTCCTTTAGATTACTGACAGAATCAACTAAAAAAGCGATCCGCACTATTATTCTAGAACTTCTGTTACAGTGCCTGCCCCTACAGTTCTGCCACCTTCACGAATTGAGAACTTACTACCTTTTTCACAAGCAATAGGAGCAATTAACTCAACAGTAATAGAAGCATTATCACCAGGTAGAACCATTTCAGTATTTTCAGCTAAAGCAATAGAACCAGTTACATCAGTGGTACGGAAATAGAATTGAGGACGGTAACCGTTTAAAAAACCAGTGTGTCTGCCACCTTCTTCTTTCTTTAAAGCATAGATCTCAGCTTTAAATTTCTTGTGCGGTTTAATAGAGCCTGGTTTTGCTAAAACTTGACCTCTTTCAACTTCTTTACGTTCAACACCACGTAATAATACCCCAGCATTGTCACCAGCCATTGCTGAATCAAGTTCCTTTTTGAACATTTCAATTCCAGTAACAACTGCTTTTCTAATTGGTTTTAAACCAACAATTTCAACTTCTTGACCTACTTTGAGTTCACCTCTTTCAACTCTTCCTGTAACAACTGTACCTCTACCAGTAATGGTCATCGTATCTTCAATTGCTAATAAGAAAGGTTTATCTACTTCACGTGTAGGAGTTGGAATCCATTCATCAACTGCTTTAATCAAATCATGGATCTTAGCCTCCCACTTTGGATCACCTTCCAATGCTTTTAAAGCTGAGCCATAAATAATAGGAGTGTTCTTACCATCAAAACCATAGGAAGTTAACAGATCACGTACTTCTTCAGCAACAAGTTCTTGTACCTCTTCATCACTAGCAATATCACACTTGTTTAGAAAAACTACCATTTTAGGAACCCCTACTTGGCGGGCAAGTAAGATGTGCTCGCGGGTTTGGGGCATCACACTATCAGTTGCTGAAACAACTAGAATAGCTCCATCCATTTGTGCAGCACCTGTGATCATGTTTTTAATGTAGTCAGCATGTCCAGGACAGTCAACATGGGCATAGTGACGTTTGTCAGAAGAATATTCTACGTGTGCAGAGTTAATTGTAATTCCCCTTGCTTTTTCTTCAGGGGCTTTATCAATTTCATCATAACGCGTTGCAGCTGATTTTCCTTCCTTTGCTAAAACTGTACAGATAGCAGCTGTTAAAGTGGTTTTACCATGGTCAATGTGACCAATGGTACCAACATTGACATGTGGTTTGGAACGGTCAAATTTCTCTCTTGCCATTTGTTTAAATTATGTTGTTAATTTTAAATAAGTTTAAGATAAGCTGGAGATAATGCTTATAATCTTCAAGGACTTAAATTTAGTGAAAACAAACAATTGCCAAATTATTGGCTGATGAAAACAACTTTCCTTACCACAAAAGACATTCTTAAGTTTCATTCCCTTATTTTTGGTCACTAGTGCTTTTGTTTTAACTGGAATTGTTGAAAGCCTTTTAACATTTGGAACTATTATTGAACAAATTGATAAATTCACTGATCAGACTAATGTGATGTTATTAATTTATGCAGTTATCTACACTTTTAATCCAAAAAGTTGATTGTTAAAAAACCAACAATTCTTTTTAAGTGCATTAGCTTATATATTATTTACTTTTATTGGCTATAACCTAATTTTGTCAATAGCTGGTATAGCTTATAAATCAACAAATCCATATAAGTTAACAAGTAGTATTTTTCTCCATGTAATTGCACCAATAGCATTCTTCATAGCAAGTTTTATCAAAATAAAACATGAGAAAGATGTCAATATTAACATGTTCTTTAAAAGCCTATTATTATTCATGATCTATCCTTTAATATATGGGCTTTATTTAGTAACTATTCCATATGTAAGGCATTATCTTTTTAATGGTAGGCCATCTACTTATACAATTTATGGCAGCATTACAAATACTAAAGATAATCCTTTTGCTTGATTAGTTGTATTTGCAGTTTTATTTATCTATTTCCCCTTGAGTTACTTAGCTATATATCTATTACAACTTAAGTTAATAAAAAAAGCCATAAAACCGCAATTTAATTTGCCTTTTACATTAAATAAATGAAAACAAAAATAAGAAAAGCAGTTATTCCTGCTGCTGGGTTGGGTGTTAGGTTACTACCAGCAACAAAAGCAATTCCCAAAGAGATGTTACCATTGGTAAATAAACCTACTATCCAATACATAGTAGAGGAAGCAGTTAAAAGTGGCATTGAACAGATTCTTGTCATTGTTTCATCCAAAAAAACAGCTATATTAGATCATTTTGATTATGATCTGATCTTAGAAAATGCCTTAATTCAAAAAAATAAATTGCAGGAGCATAAAGAGATTGAAGATATTGCTAATTTAGCACATATCTTTTTTGTTAGACAAAAAAATCAAGATGGTTTGGGAGATGCAATCTTGTTTGCTGAATCTTTTGTTGGTAATGAAGACTTTGCAGTATTGTTAGGTGATGATGTTGTTTTTAGTAAAGAACCTGCTTTAAAACAATGCTTGGAAGCTTATTATGAAACTAATTGTCAAACAATCGGTGTACAAGAAGTAGATCCTTGTCATGTTGATAAGTATGGAATTATCACCCCTGAAGGTGATTACAAAAATAAAGATCTTATTAAGGTTTTAGCAATGACTGAAAAACCTAAACCAAAAGATGCTAAAAGTAATTTAGCAATCTTAGGGCGATATGTACTCAAACCATCTATTTTCAAAGCACTTAGAAGTGTACCTTATGGAGTTGGTGGTGAGTTGCAGCTAACTGATGGTTTAAATTTTTGTTTGAAAAATGAAAACTTTTATGCAAGAAAGTTTACTGGTACTAGGTTTGATGTTGGCACAAAGAGTGGTTTTATTAAAGCAAATTTATTTACTGCTTTAAACAATAAAGATATTAGTAAAAAAGAAGTTTTAGAACTTTTAAATTTAGTTAAAGCTTAATACCATTTAAAGTTAAACCAAGGAAGTTTTCACTTCTAATCAATCGACTAAAAGGACACATTTCATGGGCTTTTTTGATTAGTTTTTTACCAACTTCTTGGTCATTACTATTAGTAGTTAATTCAACACCAGCTTTAAGGTGAAATAGTCCATTTTCTTGATGGAGTTCTACTTTAACACTTACAACTGGTTTTTTTGAAAAACTAAATTGATGTTGTTGCATAACAACAATTACTGCTTGAGAAAAACAACTTGCATAAGCAGATGCAAATAACTGCTCAGGATTATTTTCTGTTTGAACTGATAAATCAGGTTTGGGAAAACTAAGTTTTGTTTGAAAACCATCTAAAGTTTTAACACTACCTTCTCTCCCAGTTTCAGTTTGTGCAACAGTTTTATAAATCAATGCCATGTTAATTAATAATGATAATATTTTTAGTAAAAATGTTAAATAACATATTGCAATTTCTCAAAGAAAGAGAACTTTATTCACAAGCTAATTTTGAAACAGAACTAGATAACCATTTAAAAGAGAAAAAAATTAACTTTTATGTTGGTTTTGATCCAACTGCTAATTCTTTACATATTGGCAATTATGTTTTAATTCACATTGCAAAATTATTAAAAGACATGGGGCATACTCCGCACATAGTTCTGGGGAGTGCAACTGCTTTAATTGGTGATCCTACTGGCAGAATTGAATTAAGGAAAATTTTAGAAGAAAAAGAAATTGTAAAAAACACCAAAACAATTAAACAACAAATCAAACAGTTTTTAGGTGATGTAATTATTCATGAAAACAAAGTTTGATTAGAAAAACTTAATTACATTGAAGTTATCCGTGAATTGGGTGCTTTTTTTTCAGTTAACAAGATGTTAAGCACAGATGCATTTAGTGTTAGGTGAGAAAAAGGGCTAACTCTAATGGAATTAAACTATATGATCTTACAAGCATATGACTTTTATTATCTACATAAAAACCATAATGTCACTTTACAAATAGGTGGAAGTGATCAGTGGGCTAATATTTTGGCTGGTGCTAACTTAATTAAAAGAAAAAATAATGCTAATGTTTTTGGATTAACTGCTAATTTATTAGTTAAAGCTAACGGAGAAAAAATGGGTAAAACTAGTAGCGGAGCATTATGACTTGATGAAAATAAAACTAGTGTTTTTGATTTTTATCAATACTGGATTAACCTTGATGATCAAAGCTTAAAAAAGACTTTTTTAATGCTAACAATGCTTGATAAAAAAGTAATAGATGAATTGTGTAATTTAAAAGGCCCAAAAATTAAACAAACCAAGCAAATGCTAGCCTTTTTAATTACTGAATTAGTCCATGGCACTAAAAAAGCAAAAGAAGCACAACAACGATCTGAACTAATATTTAGTAATCAACCAGATCTTGATATTAAGTTAGTAAAAACAAGCACTAATCTGATTGATTATTTAGTTGAAACTAAATTTATTAAAAGTAAATCAGAAGCAAGAAGATTAATTAGTCAAAAAGGTTTGACAATTAACAATAAACACGTTTTGGACTTAAACCAAATAATTGAATGAAAAGAAGAGTTACAAATTATTAGAAAAGGTAAAAAAGTTTTTTAACAATTAAAGCTGTTAATTCTTAGCTAATTTATCTTTAACTTCTTTTTCTATTTCAGTTTCAACACTTTTTTGACTAGAGCTTTTACTTTCTGGTTTTTTGATTAATTTGTTATGAAGTAAATCAACAACTTTCTGCATTGAATTTAATGTTTTGAAATCATAGCTTGCTTGGAGTTTAATGTTTGTAACTAGATCATCAAATGCTTCATTCACTTTTAGTTTAGTATCCATAAAGGTTGTAGGCACACTCATAAACAAGTAAGTATGAACTGTGTCATTATAGACATTAAATTCGGCATATACATTCAAAGGATTAAAAAGCACTTTATTTAATACCTCTGTATCTAAATTCTGAAAAGAAAAATTTGAATCTTTTTCAACAAAAAGTGAGATGTTTTCTTGCTTTTTGTTTTCAGTAAGTGCTTCAAACTGAGCCACATTCTTATTTAAAGAATAGTTAGCATCAGTTAAAAAGAAATGATTTTCACTATTAATTGCTGGTGTAATAGCAATCAAGCAAGCTACTTTTTCTGTTTTATTACTGCTTCTTTTAATTGCATAAATATGACCAAAAACAAATTCACTACCATCTTTATAAGTAACTTTAAATTGTCTTGGAAGTGGTTCAAAATTGTAAAGAGCAGTTGGATAAAAACTTGGTAATATATCGCTGCTTATAGGACCTTGTTCAAAAAATTCAAATGATTCTATTGCCTTGAAATTACCCATCTCTTTTTGTCATAAATTAGTTCTGTTTTCTTTGTTAACTTTTTTTAATAGCTTGTTTCTTTTAAAAAGAGCAGCAAAATGGATAACAAATGCTAAAAACAAAACAACACTACCTAAAACAATTAAAAAAAGTGATGCTATATTTGGTATTTCATTACGTGTATAACTATAAATACCAAATGAGATAGCAATAACACTAAAAAGACCACAAAGTCCAATTACTCTTAGAAAAGCATTGAGTTTATTTGGTGTCAAAAAGAACTTGTTAAATTTAGTTAGATCTGACATATTTGTATAAAAGATTGTATGAAAATATAAACCAAAGCAAGCTCAACAGTTGTTTTATTACAAGATCTTTTTCCTAAAAACTGCAAGTTGCCAAACTTAGCACGCATCTTTATTAAACGAAGATAGAGCTCATCAAATTTTATATCAAGTATTTTGTATCTTTTGTTTGTTTTGGAAATAAAAGTGCAAAAGTACTTTAGAAAATAAAAAATCAGATCCCAAATTCGTTTTTGTCCTAAACTAATAGTTTTGTTTTTTAGTAATACTTCAGTTTGCTTTAAAACATTTTTAAAAGTAATAACAAGATCATCATTACTGTCATTTAAATAAGTTTGTATGTTTTCAATTCAAATCAAAATGTAGTGATATTCACTGTCAAACAATTGGGGATTATTTTTAATTACTGTAAAAAAATCAGTAATTGTTTTACTTTGATCATTTAGTACTTTAAAACAATTCCAAGCTTCATTACCATAACCAACTTCTATTGCATTTAATTCTTGCTTAAATGATAGTAAGTTAACCAAGAAGTTATCAATAAAATCAACAACACTAACTGTTTGTTTCACTTTCTGGTTTTACTTCAGATTTATTTAGTTTTTGCTTTGCTTGTTGTTCTTGCTTTTGCAATAAGATTTCTGGTGGTAGTTTAGTGTTTTTATGGATGAAGTCAATATCACTTTTCAAAATAGTTTCAGCAATTAAAAGTGCTTCTACAAGCAATTCTAGTTCCTTACGGTTACTCTTAATGATTGTTTTTGCTTTCTTATACTGTTCTTCAATAATGAAATTAATCTCATTGTCAATATCTTTAGCAGTTTGTTCTGAATAAAGTTTTACATTAGAAGGGAGTGTCCCTTGACTTGGTACATATTGCACTTGACCTAATTTAGACATCCCAAGCTGGGTTACCATTGCTCTTGCAATATTAGTTGCTTTATAAAAATCGCTAGAAGCGCCAGTAGTAATTTCTAAATTACCATAGATTTCCTCTTCAGCAGCTCTACCGCCCATAGCAGTTGCTATCATTGCAAGTAAATCAGATTTTCTTTTTAGGTTAAGATCACCACTCTTAGGTGTTGAAAGTGTGTAACCCCCTGCTTGACCACGAGGAATAATGGTAATCTTTTGTACTTCATCATTACTGTGGACATGTAAACCAACCAAGGCATGACCAGCCTCATGATAAGCAACTAGTTTTCTATCTTCATCACTAATTACACGTGACTTTTTAGCAGGACCAGCTATTACTCTATCAATTGCTTCATCAATGTCATTAATGTTAATTGTGGTACGGTTGTCTCTAACTGCTAACAATGTAGCTTCATTGATAACATTTTCTAATTGAGCACCTGAAAACCCAGGAGTTCTCTTAGCAACATCTAAAAGACTTATCTTAGAAGAGAGATTTTTATTTTCAGCATGAACTTTTAAAATCCCTTCCCTTTCTTTAATATCAGGGAGATTGATTTGAATATGTCTATCAAATCTTCCAGGTCTTAATAATGCATCATCTAATACATCTAACCTATTTGTAGCTGCCATTACAACAACACCTGTTCTGCTTGTAAATCCATCCATTTCAGCTAACAATTGGTTTAAGGTTTGCTCAACAACAGAATAAGAAGAGAGTTCAACTCTACCCCGTTTAGAACCAACTGAATCAATTTCATCAATAAAAATAATACAAGGAGCAGCCTTTTTAGCTTTATTGAAAAGATCTCTAACCCGTTTAGCACCAACACCAACAAGCATATCTTCAAATCCAGAACCCGTTGATTGAAAGAAAGGAACACCAGCTTCACCAGCTACTGCTTTAGCTAATAATGTTTTACCTGTCCCAGGTGGACCATATAAAATTACCCCACGTGGGGATCTTGCTCCCATCTGGGCATATTTCAATGGATTTTTTAAATAATCAACTATCTCAAGCAACTCATGCTTTTCCTCTTGAAGTCCAGCAATATTGGTAAATTTCACAGTTGACTTAGCCAACTTAGCTTGGGTTTTGCCAATAGAAAAAATATTATCTTCTTCTCTGCCCCCTGCAGATATACCCCTAGCACTTCTTCAAAAGAGTAAAAAGAAAGCTACAAAGATAATAATTGGTAGCAAGCCAAACAAACCATTTAATACATCTCTAGCTCTAGTATCAGGAGCAATGAAAGTACCAAGGGTTTCAAAACCTGCAATACTCTTTGTGTTATTACTAGAACCACCATTCATCATGGTTCCATTACTATTAACGGTAATCTGACCATTACTTTGGTTAATTGATAAGTTAGCAATATTAAAGATAACATTGCCATTACTATTAACAGTTTTGTGAGCAGTAACAGTTAATGGACTATTTAAACCATCAAAGGTAATGGAAACTTGGAGAATGGTATCAGTAACATAAGTTGAACCATTTATTTGTTTAAATGTCAGTTCATTACTAAAACCGCTTACTTTTGCTGTTAAACTACTACCATTGTTTAATTTTCAGCTTTCAACTACTGCCGTTGCAGCTCTTGGACTGAAAATATAAGCTAAAACACCAATAACAACAGCTAAAATGATGACTCATCAAAAGACTTTTCAAGCAGTTTTACGTGAAAAATTATTTTTTTCAGTAGTTGTTTGTTCTACTAAACCCTTATTTCTTTTTTTCATCTATGTTAGTTTTTGACCGATTTGGGTTTTTTTGGTTTGAACTACATTAACTGTAATTATACTGCCAATGGTAATGAAACTAATAAGTGTTAATAAAGTGACGAAAATTATCTTTTTATGTTTACTTAAAAGTGCAATAAAAGCCTTGAACCAACGCATAAAATTTTAATATCTGTTTTAGGGATTGTCTGGTTCAAACACACCAACATAAGGTAGGTTACGATAAAAACCATCATAGTCAAGACCAAAGCCAACCAAAAAATTATCTTTTACTTTAAAACAAGAAAAATCAATATTAATATCAAAGGCTTTGGGTTTAATCTTTTCAATTAAGCTAATTAAAGTTATTGATTTAGCATGCCTTGTTTTTAGAAGATCAATAACTAATTTAATAGATCTACCACTATCAACAATATCTTCTATTAAAAGGATGTCTTTATCTTTAGGGTCATGGGACATATCAAGCACAATCTTAGGTGGTTGTTTTTGTACATGTGAACCATGATAAGAAGCAACTGCCATAAAATCTAGTTGGAGGTCAAAACTAAATTTACTTATCACTTTGCCAAGGAAAGGGATGCAACCTTTTAGAATGCCAAGAACAATTACCTTTTTATTATTAAATTTAGCATTGCACCAATTAACTGCTTTTTGACAGCCTTCTTCTATCTGTTGTTCATTAATAACAATAGATTTAATACCCATCTTATTTAGTTTTTTGACGTTCTACTAAAACAACAGCTAAGCAATTAATTTCATTACTGTCTAAAGGTTTTCTAACTTTGAGATTAATTGTTAATTCTGAAATTCTTAAAGCACTACAAATAAAAGCAAAGATAGCGTGTTTATAATCATCAACCTTTTGCTCTTGAGGAATCTCTAAGGAGATATCAATATTATTGATAATTCAGTTTTTTCTGATGAAATTGTAAGTTCTTGCTAAGACTCTTGGTGCTTCTTTTTTTGCAGTACCTTTAAATTGGACTGTTGATTTTTCTTTATTGAAAACAATATGACCATCACCTAATGCAGTTGCACCAAATAAAGCATCAGCAACTGCTAATTGGATAACATCACTAACTTCATCACTGTTTTCAAGATCATAAATATATTTAGAATTCTCAAAATCAACACCGCCTAACCAAAATTTATTTTTACTTTCTTTCCTTAATTTTATGTGGTATTTTTTGCTTCCTAGGCCAACTCTTAACTGCATTGTTACAAATAATTATAATATCGCTCATCTTTAAATAACAGTGTTGTTTTGTAATCCAAAAAAAGTAAATTCAGGCAACAGTTTTTTGACTTCAAATATCCTTATTTAGACAAAGCAAAATTTATTAAAGTGAACCAAAATAAAATTGAAGTATATCTCTAGATAAATAAATATGAAAGGACCTAAAATTGCAATAGTAGGATCAGGTGCTGTTGGTACTAGTTTTCTATATGCTGCAATGACTCGTGCACTTGGTAGTGAATACATGATTATAGATATCAATGAAAAAGCTAAAGTTGGTAATGTATTTGATCTTCAAGATGCTTCCTCATCATGCCCAAATTTTGGCAAAGTAGTTGCTGGAGAATACAGTCAATTAAAAGATTATGATTTTATTTTCATTAGTGCAGGAAGACCTCAAAAACAAGGTGGGGAAACTAGGTTGCAGCTACTTGAAGGCAATGTAGAAATTATGAAAAGCATTGCCAAAGAAATCAAAAAATCTGGTTTTAATGGAGTTACATTAATCGCTTCAAACCCAGTTGATATCATGTCATATACATATCTTAAAGTTACTGGATTTGAACCTAATAAAGTAATTGGCTCTGGTACTTTACTTGATAGTGCAAGATTAAGATATGCAATTGCAACTAAGTACCAAATGAGTTCAAAGGATGTACAAGCATATGTTATTGGTGAACATGGTGATAGTTCAGTAAGTATTATTTCATCAGCTAAAATTGCAGGACTATCACTAAAACATTTTTCTAAAGCTAGTGATATTGAAAAAGAATTTGGTGAAATTGACCAATTTATCAGACGCAGAGCTTATGAAATTATTGAACGCAAAGGTGCTACTTTCTATGGAATTGGTGAAGCTAGTGCTGATGTAGCTGAACAAATTTTGAAAGATACTAAAGAAGTTAGAGTAGTGGCTCCTTTACTTACTGGTCAGTATGGAGCGAAGGATATGATGTTTGGAACTCCTTGTGTACTTTCAAGAAAAGGTATTGAAAAGATCTTGGAAATAGAACTTTCAAATACTGAAAAAGTTGCGCTTGAAAATTCAATTAAAGTTTTGAAAGACAACATTAAACTAGCAAAGCTTTAGTTTTGATAGAAAACATTAGCTCATCTATAAAAAGAGTTTTCAAAAAATAACTCGTTACTTCTCTTAACAAGCATACTAATTTCACTGAGATTTTTAATCTTGTTTTGAGAAGTTTTTATTTTTAATTCATTTTTGTGCTTATTTCATCCTTCATAAAAACCCTTAAATTGGTTATTGAATTTAGTAATGAAATAACTAGGAGTTTGATATTCTTTCACTTTTAATTCAAAATCAAGATCACGTTTTTCACAACTTTCATAAAACTTTACTTGAAATTTCAGAGTGTTAAGATAACTATCTAGTAAGGTTTTTAATATTGCATCTTTAGTTGTTTCAAATGTATTAACTAAAAATGAGTGAAAAACATAATCATCTAACTTCACAAAACAAGTTAATAAACTTTTTTTAAACAACTGGTTTTTTAAAAGCGGTTTAATTACACTTTCAACATTCTTAAAGTTATGAAAATTAAAGTTGTCTTCTTCAATTAAAGCTTTAATCCGTTGAAAAACAAATCATAAGTTAAGTTCTGTAGCAATACTTCTACCATCACTGTAGATTGATTGATACATGTGGTGTCTTGTTATCAAGAAGTTTTCAATGAAAGGTAAGCATTTTTCTTGAAAAACAATTTCATAAGTGCCCTGATTATCTACACATTCCATTCCATTGATAATTGATTGATAATCAACTAATGAATGACTGGTACCTGTAAAATAAGCATCACGTAATAAATAATCCATTCTGTCTGTGTCAAGATCTGAAGATATTAGCTGTCTCATCCACCAGTTAATAGGTTGAATATTTTGATTTTCGTCAATTAAAGCGCCAATCAGATTTGGATCTATCTTATTAGCTTTTAAGATAGAGACAATAGGTTCACTATTAACTAACATTGAAGTAACCTTTTCATGAATAAAAAGTTGTTTTTTAAAATCAGGATTTTTAGCAAAATAGATTTCAAAAGCATGAGAATGAGGTCCATGACCAAGATCATGCAACAAACCAGCTACTAAAACAGTTTGTTTTTTAATTTGATCAATATTTAAAAATGCAGAACTATTTAAGATCCTTCTTATCAGTTCATAAACTCCTAAAGAATGGGCGTACCTAGTGTGAACTCCACTAGGATAAAAATGGAAGTTAATTCCTAATTGTTTGATGTTTCTTAACCTTTGAAAAGCTTTTGTATTGACCAATTCATACATCCACTTTGTGTTTTCATCAAAAATAATTTCTCCTAAAATAGGATCTTTAAAAAAGGTTTGTTGCATTAATAAATTGCTTAATTAAGTTTATTGCACTTTCTTTATCAAAAATTTCAATAATATGTGCTAAATCAGGTCCATGCTCCTTATTTGTAAAAATTAACCTTATTGGCATAAAAAGTTGTTTACCTTTTAAGTTAAACACTGCTCCTACTTTGTTAATAGTTGTTTTAATTTGCTCAGCTTTTCATTCTTCCAACCCTTGTAAAGATTTGGCAAGCTGTTCTAAAAAAAGTTTGATGTTTTTAAACAAAATATGACTTTTCTTAGCTAAGTTTTCAACACCTATTTTAGTGGCAAAAGATTCTCTTATCAATTCGTTTATTTGAACACCATGGGTTATTTGATTTTTAAAAAGTAAACTTATTTCCCTGTTTTTATCTTTTAAATAATCAACCTTAACATCCAAGTAATTATCAATGAAATTGAAATAAGCATTATCAGTTAATTGTTTAATGTAATTAGCATTGATTCATTGCAGCTTTTTAATATCAAAAAAAGCAGGAGCACTAACAACTCTACTTAAACTAAAGTTTTCAATTAACTGTTTCAAATTAAAAAACTCCTGGTTGTACTGTGGATGTCAACCTAAGAGTGCTAAGAAATTTAATAATGCTTCAGGTAGATAGCCTTGTTGTTTAAACTGCTCAATAAACTGAGTAGTTTTCTCATCACGTTTAGAAAGTTTTTTGCCACTTTCATCAACAATAACTGAAAGATGACCAAAGCGGGGAATTCTTTTAAAACCTAATGCTTGATATATAGCAAGTTGGTATGCAGTGTTAGAGATGTGCTCAGCTCCCCTTAAAACATCAGTAATTTCCATATCATAATCATCAATAACAACTGCAAAGTTATAAGTAGCAACACCATTAGCTTTAAGAATAACTATATCTGTTAACGCACTGCCGGGAATAGTAATTTGTCCCCTAACCAGATCATTTCAACTATATTCAGCTTCATTGTTTATTTTTAAGCGGATAGTAAAAGGATCATTTTTTTCTAAGTGATTAGTAATTTTCTTGGAATGTAAATTACGACAATGACCTAAGTATTTAGGGGTTTTGTGGTTATTAATGGCTTGTTTTCTATCTGACTCTAATTTTTCTTTGCTACAGAAGCAACGATATGCCAGATTTTTTTCAATTAAATCAAATGCTAGTTTTTTATAAACTGCTAGTTTTTGTGATTGCAGATATGGACCATAATTGCCAGGGTTATAAACCGATTCATCTGCAATGACTCCAAGCCAACGCAAGTTATCAAATTGTGAATTAATTCCTTCTTTTATGTTTCTTTCAGTATCAGTATCTTCTATCCTGATAATAAACTCACCATTAAAGTGCTTGGCTAGTAAAAAGTTAAAGATTGCTGTTCTTGTACCACCAACATGCAGATATCCTGTTGGGGATGGTGCATAACGTGTTCTAATTTTTTCCATTACAAATGTTTTGGTAAAGATTAAAGAGTTGACAAGGACTCAACTCTTGCGCTCTAATGCTAGTCTTTAAATTTTGCTTTTGAATAATGTTAAGTAAATAATCAACTGCAAAAAAATGCTTTAAGTTATTAATTAACATCTTCCTTCTTTGATTGAAACATTGCTTTAAAAACAGACCAAACTTAAAGTCATAACTAACCGATTTATTTTTTTCTAATAATATTAAGGTTGAATCCACCTTAGGTTTAGGTTTAAAAGCATGCCTATCAATTTTAAAAACTGTTGTAATAGTCAAATAGTATTGACAAAAAGCACCAAAGGCACTATAATCACTAGAATTAACCTTTGCCAGAAGCCGATTAGCAAACTCTTTTTGTGTCATTAAAACAAAGCTTCGAAGCTTTGATTCTAAAAACTTATTGATTATTGGAGATGTAATGCTATATGGGATATTACCACACAATAATGGACTTAAATTTTCAAAAAAACTATTAAAGTCCTTTTTGAGAATATCGCCTTTAACTAGTTGGTCTTCAGTTAATATCTTTTCAACTAGAAGATATTCAATTAAGCGTTTATCAATTTCTATCCCCTTGTAAGGTATTTTGAGTTTTAACAAATAATTTGTTAACGCTCCTTTACCAACACCTATTTCAACAATTGCTTGTGGATTTAAATTTTTAACAAAAGCAAAAATTCTTTTAATGACGCTTAAATTAACCGTAAAATTTTGACCTAATTTACGTGAAGGAAAAAAACTATTCACGCTCTAAAATCAAATCTAATTTGGAATAATTTTGCTTTCTTTTTTCCCTTCTTTTGACAATGAAAACATGGGTTAAATAAGATTGTAATAAAGTAAACAAAGCATTTAAAAACCAATAAACACCAACTCCAGCAGCACTAAATGCAGTAATAGCAGCAAACACAAAGTAAAAAATTAATTGCATCTTTTTAGTTTTGTTGAGTTGTTCAATACTTTTTTGAGAATGGGCTTTCGCATTCTCATTACGCTTACTTGCCCACACTTGAGGAAGTTTTTGAGAGAGAAATTGGACTGGTAAAACGATCACTAAAAAGATGATGAAAGGTCAACCAGTTGTAGTGAAATTAGAGAAAATTTCTGTTAAAGGTACTTTTGAAAGATCCCAAAAGTTAAATAAGATGATTGCTTTAATTGGTCTTAAAGTAGTAACAATCCTATAAATAATTAAAAAGATAGGTAAAGTTACAAAAACTTGAACAAAAGCAGCAGATGATTTAATGTTGTGCTTTTTATAAAGTGACATTATCTCTAACTGTCTGTTTCTTTTACTTTGTAAATCTATTGCCCCTTTATATTTAGCGTTAATTTCTGCTAATTTACCTTGCACTTCATTCATCTTTTCAAGTGCTAAAGTAGAGTTTAAGGTAATCACAATAGTTATCAAGCGAACTAATAGTAAAAGTACTATTAATGAAAGAATCATGTTAAACCCAAGTTCCACCCCGCTACCTAGTGGAACTCTGGTTGCATACATAATTGGTAAAACTATTTGCGCTGCAGGCCAGACAAATCATCCATAAAAAGGGCCATATGCTAAGGTGTAATCACTAAAGGTGAAATAAGGGCCAAAGTTATTTGAAATCAAATCATACCTATAATCACCAGTAGTACCAAATTTATAACCTATCTCAAGTCCTGAACCTAAGACTTGATTAGTACCTGTTCAGGGTTGTGCTAAGGTTTGAGTACAGCCCCAAAGCCCAACAATAGTTAAAAAAATAAAGATAAAAACCTTAATAACCTTTATGAAAATAGCTGAAAACTTTTTGAAGTTTTTTCAGTTGTTTTTTTCATTAACAACTGCTGCTGATCAAAAAGGATTAAAGGTGGTTTTTATCTCTTTATTTGTTTGCGCTAGTTTGATAGGCATCTACTTCTTTTATCCGCTTTAATAGTTGCAAAAAAAGTTTTTGTTTTTCTTTAAATGTTAATTCAATAAAGCCTTTGTTGACAATAACTAAAATATCTCAAGGTTCTAAATTATTACTAATTTGTTGAAAGATACTACGGATCTGACGCTTAATTAGGTTACGTTGTACTGCTAGCTTATATTTAGTTTTTGCAATTGATATTGCAACCCTTCAAGTAGAATGATTATTCTTAATAAAATAAGCGTTAATAAAGGTACCAAAGAACCTTGTTTTACTTTGAAGAATGGTTGTAAAAACCTTGCGTTCTCTTAAGCTGTGACTCTTTTTAACGCTCACTGGAAACCGTGAGTTGAGCACGATTTTTAAAACGTCTTTGCCTTAAAACTTTACGTCCTTGTGCAGTTGCCATCCTAGCCATAAAACCATGGGTTTTAGCACGCTTTAATTTGCTTGGTTGGTATGTTCGTTTCATGAAACAATTAATCAGGTGATTATAACTAAACTAAGGGTCTGACTAATTGGTTAGATACTATTTTACCATCTGAAATTTTAATGATTCTATTAGCTATCCTTGTTAGGCTTTCATCATGGGTAACCATTAGGATGGTTTGCTTATATGTTTTGTTAATTGTTTGTAATAAATTAATGATATTTTCCGCAGAATTACTATCAAGTGCACCAGTAGGTTCATCACAAAGTAATAGTTTAGGTTCTTTAATAATAGCACGGGCGATGGCCACTCTTTGTTTTTGCCCACCTGACATCTCATAAGGAAATTTATACAAGATTTCTTTAATATCTAATTTTTCAGCCAACTCTTCTATATCAAGTCGTCTTTGATATTTGGGAATTAAGTTTTGTGAAATGGCAATATTATCATAAGCACTTAACAATTCAATCAGATTATAGCGCTGATAGACATAACCAAGGTTATTCTTTCGATAAGTTAAAAGTTGTTCACTAGTACATTTTTCTAATGGACAGTTTGCTACGATACAACTCCCTAAAGAGATAGAATCATAACCACCTATCAGGTTTAAAAGGGTTGTTTTACCTGAACCTGAAGAACCTAAGATGATTACTATTTCACCATAAGCTATCTGTAAAGAAACATCTGAAAGGATAGCAATCTCTTGCTTTGAATCATCATTATGAACATTTTTTCACATCTTATTGATGGTAATAACAATTCCCTTAGCTTGTTGAGCAAAAGGAGGGATATATAAGGTATTGGCTTTTTGCTTTTTATTAGCTTTCTTTTTGAGCTTTAGCTCAGCTTTTTTAATGTTATTTAAAACTTTTTTATTCTTCTTAGGAGGTTTAGATTTACTCTTTTTGAAATAATCATCGAAATTGGCACTAAAAGAAGGACTTTTAACGTTATCTAAAATATCAATACATTCATTTCAAACAGTTTTATTGCCTTTTTTCATTAACTAAAACCCTTCCTTCAGTTCAGCTGAGCGTAACTTAATTCAAAAGTAAGAACCAATTACAAAGATAGTAACACCAAATAATACTATCCCTACCATGTATGGCAAAAACTCAAATACATTAGTTAAAAAGACTTGTGCAACATTGAAAACAACTGCTTGGATACCAATTAAAACACCAGCTAAAACTCCAATAGAAATTAGTAATGCTAAAACAAATGCAGGGATAAAGAAGGTAAGTAAACTAATTAAATTTTGTACATCTCTATAGCCAATAGCCTTAAGTGAAATAAAGATTCTTTTCATCTCTTCTAACATTGAGATACCAAGTACAACACAAGTAATGAAAATGAAAGGAATAATAGTTCCTAAAAGAAACGCATCTACTTGATTCACAGTGTTAAACAAAACTTCAGCATTATTTCTAATGATCTGAGCACTAGTTCCAGCTGCATCAATATTGAGTACAGGAGCAATAATGTTATTACCAAAAATACTACTTGCATTTTGGGAAAGTAAATTAACACTATTTCATTCTGTTTTGTATTTATTAACTAGTTCTTTACGAAGCGATTCATAATTCATTGAAGTAGTATTAATAATCTGTTTTTCTTGTGCTAACTTATTAATAATTTCAGGATCAGTAGGTAGTATGGATGTAATAACTTTATCAAGTTTATTCCTTACTAAATTTTGGAAATTATTACCAAAGTCAGTGAAATTACCCCAAACACCAATTATCGAGTTTAATACTAATGCCTTATTTAATAAAGAAGGTTTTAATTCTTTGGAAAATACACCATTAAAAGGAACATAACCGTTTGGCTTTACATAATCAATTTCATATTCATCACTACTTTGAGATTGGCCATTTGTCTTTTTAATAACATCTCTAATAATACCTCTGCCCTGATTACCACTTAGTTTTAATAAGTTATTAGCAATGTGTTGGTTAATATAAAGTTCTTGTCCAAAAGCATCAGTAGAAACACCAACAACTTTAAATGCTACTGTTGAATTATTTAAGGGTGCTTTTAGAAATTTTTCAGAGTATCTTGTGGCAGTATTTTTCGGTTTTATATAAACATAATCACCAATATTAATGTTGTTAGCATATTGAAAACCAGCATTAACAATTACATTCATTTCCTGATCAGAACTAATTAAATTTCTTAAAGAGTTACCACTACCATCTATCAGATTAACAAAACTTGATTCAGGGTTAATCCCTGTTAAAGTGTATGGTGAATTCTCATCAAGATTATCTTCTTGAAAACCAAATGGGCCTTCAACATAAGTGTAGGTTTCTTCTATGGCAGGGTCAACAGGAACAATCCCAAAATTTAACTTAGAATCTTGTTTTGCAAGTTCAAAATCCCCATAAACTTTCAACATAAAACTAAGAAAGCTATCATTAAATTTAACCCCACCAGGATTAAATACATCAATTGTAGTTAGTACCCTACTAGCATCTAACTGAATTTGATTAGTTCTAGGGTTTCTAATTAAAAACCCATTAGCATTTAAGTTATTGAAAAAGTCATCAGTTAATACTGAAATTAAAAACTGATCACTAGATGCTGAAAGTAACTGAGTAATCTGTTTAGGAATTGATTTTTCAACAATTTCCCAAGGGTTAATGTTAGAACCGCCAACCCTAATAGGAAAGTCAATAATCCATTTTGATAGAGAGGCATGCCTAAAGAAATTCTCATCAGTGTTTAACTGTTGAGCACCACCATAACTAGGCAAAAGTAAGTTGCCCAATTCAAGCTCAGTTCCATCTGCTGCTTTTATTGGTTGATCCTGGCGGTTTTTAAGTTTTAAATTACTTGGTTTATAAGGGTGCTCATTTTGAGAATTTGCTTGAACACTTTCGTTATACAAGGTTTTAATACCATTATTATTATCAGTAACTCCAAAGTGTGAATAGGGTTGAATTGCATACCATCCAGATTGCTCTGTTGGTGTTTGTAAATTCAACTTATAGTTAAAGTTTCTATTTAAGGAAGTGCTTGTTTGTGCTGCACTAAATTTTTGGGGAATAGTACCTGCAATCCCAATTAATAAAAGTGCAACTGAACTCAATCCTACATAGGTAAATAAACGTGAAAAACGTGAAACGATTAATGAAACCCTAAACTTGGTCAAGGGTGACATTGTATGACTTTTATGTTTTAAAAGATGAAGAAGAACTAAAAACTTGGTCTCATTGCCTTGATCAATTAAGACATTAACAGGTTTACTAAACAACTGTTTAAAGGCAATTCAACTAATAAATTCAAAAACAAAAAAGGTTATAAAGAAAGAACCAAAAAACGATAACCAGTTAAAGCTATTTTCAGGTAAAGCAATAAACCAATACCTATTTATGATTCCCTTTACTTGTCCTTCTAGAAGAAAGGCAAATAAAACTCCTAAAAAACTAGAAACAATTGCAACAATACCAGCAAACACACTCATTCCAACAATAAATTTAGTTGTTGAAAATCCCCCTGCTCTGATAATAGAAAACTCAGTTTGGTTCTTTTTAATAAATGACTTAATTAAAAGAATAATTAAATAAAGAGCAAGAATAATCAAAAATATAGCAATAACAGTACTTACTATTGCCAAATAGCTTTGCACTAAATCAGGAAAATAGTTTCTTGCTGTTGTCAGATAATTAAACTTAGAAATATCTTTAACATTAAATGCTAAATCACTTCTAGGAACATCTAGATATCCTTCTAATCTATTAGCAATAAACTGCACTGGGTCTTGGTCAGTGTGCTGTTTTAAATCTGTTGACTTAAAAGCATAATAATTTTCTTGGTTAGCTGCTGGTACAGCAAACAGAATACTTCTGTAAGCTTGATCATTAACAAAGATTAACGCTTCATCTTGGGTATTAGGCAACGGAGATTGTAAAGATAACACTGGGTAACCAGTTTCTACTGATTCACCTATACCTAAAATTGCAAATGTTAGTCCGTTAATACTAAATTTATAACGATCAGGTAGATCATTTAACCAGTTAGGGAAATTGTTTGGTGTTTGCAATGCAGTTTTTCAAGCATATTCCCCTTCCTGGGGAAGAATTTCTTTATTGTGTCTAAACAACCATTGTTGAGAAACAACCGCTCCGTGTGCTTCAGGGGTTTGCACAACTAAATTTTGTTGATCAGTAGGTAATATTAATGGATTGACCCCATTTTTAACAGTAATAGGGATCTCAAGCATTTGGCGATTTGTCTCTATTAATTCATTAGGATCTTGAAAAGCAGGAGTAAAATAACTTAAAAGTGCAAGATAATTATTAGCTGCTTTTCGTTTATCTTCTTCCTTAAGATTATTAAATCCATTGGCATCTAAATTAGTTCATTGTTTTAAAGCATCTAAAGCAGTAGTTGGGATATTAGTAGCTTCTGGTAATTGGGCTTGAGCAATAATATTTGCTAACTTTGGATAAATGGGAAATTTAGTAGATTTTGGACTTAAAGCTTGGGTATTTTTTAAACTAGCAGTCATTCCACCACTACTGGTGCTTGGTTTAAATTCATAATCAATTTTTTGTAAAAATCCATTATTAGCATCTTGACCACCATAAATTGTGTCTAAAGGGTTTGATTCATTTTGCTCAGGTTTAAATAACCAAGATTTAAGATCAACGCCATCAATTAAAGAAAGATCGTTGTATCTCTGTTCATCTTTGTTGGAAACTAAAAAACTTGCTGACTCTGCTGAACTAACATTGTTAGCTATTGCGATATTTTGCTTTGAATAATCAATGTTATTGAAAGCAGTGGCTTTTAAAAAATGATCACGGTACTTTTCTAAAAGATCAACTGTTTTTTTAACAAGATCCTCTTGAAAGATACTACGTCATTGATCAAGTACTGCTGCATAAACTGCATCAAGGGTTTTTTCAGGAGTGTTATTGGGCTGGTGAATTTTATTGTATGCAGTTTGAACATTTGAAAAGTTAGGATCAGCTATTCCATTACTTGCTTGACTGTTTTGATTTTGAACTAATTGGAGATTGTTGCTTGAAGGTTGAAAAGAAAGATTATTATTTCTTACCATCATTCCATTCTGAGTTTTAAAGATTGTGTCTTTATCACTATCACTAAAACCTGGTAAGGAATTGAGAATTGTATCTTTCTCAGATTCAACTTTCTTTTTAAGTCAATCTTTATTAATTTTTAGTTCAAATTTCTTAAGTTGCTTAAGTTTATTTTCAAGATCTCAATCTCCAAAAGAGGAATTATTGGCTATAGTTTTAGCATTAGTTTCTAACCACTTTAGCTGTTCATTATCTAGCTTATTTAATTTAGAAATATCTAAATTAAAAGTTAATGTAAAACTTTGATTATCTTTACTTTCTTTTTGGGTTTGAAATTCTTCAACAATTTCTTTAAAAGGATTGACATCTTTCCAAAATTGAGAACCAGGATCGATTTCTAAATCAATTGGGATGTTTGCATAAAGATTGGGAAATCAGTTAGCAGCTTCATTACCACCTGTAAATAGATAGTTAGCAGATATATCAGTATTATCTGCTTTGGTCTCATCACTCTTAAACCAAACTTTATCTTTGTTATTGGTCTTATAAAGTGAATTAACAGTATTGTCTTTTTGTGGATCAGTGTTTGTTTGTTGCTTTTGTGTAATTTTTATTTGTGTTCCTGTGCTTTTTCCTTTATCACCAGGAAAACGCGCAATGCCAGCACGCAGTGAGATAGTTTCTTTAATTAAACCATAAGCAAAATAACCTAGAAGGTTACCACTAGCATCATAAATAAGGTTTTGAGGGTCTGATATTATGTGTCTATTTGTAATGGAATCACTAATATCATTACCATTAAAAGAATCAGTATTATTAAAAGAAACTGCTAACCCTTTATAAATTCTTTTTTCAGCAATATTGGCTTCTCCATATGCTTTTAAAATACCTCTTTTTCTTGCTAAAAAGAGTTTATCTTTTTCATTAACATCGCCTTTGCGGGGAATGGTTTTGTTTTGGTTATCCTCACTACCATTACTTTGTCCTGAATCACTTCCAAAATCATTTTCTTGATAAGGAAAAAGATAGTTAATTTTTTTATCATCTATCTTTATTTTGGAAAAATAACCACTATCATTAGTTAAACTTGCTGTATAAACAGGATTAGCTTCCTTTAAAATAGCAGGAGCTACTATATCAGAAGAGTTAGTTTTAGATACTAATGTAGTGTAGGTACTAACTAAGTTATTACTTAACTGAACAATAGATGTTTCAAGGAAAATAATGCTAAATAAAACAAAGATAATCCCAAATAGTAAAAAGAAGAACTTTTTAAGCGACTTAAATATCTGTTTAAAAAAAGAAAACATCTCAATTAAACTCAGTTAATTTGTTCGATGGTTTTTGGATTCTGATTTAAATAATCAACGATTATCTTGCCATCATGGATTTTAATTACCCGTTTAGCTAATTCCACTATCTTTTCGTTGTGGGTAACTATTACTATAGTAGTACCTTTGTCACGATTATATTCCACAAAAAATTGCAATATTTTTTTGGAAATATCAATATTAACTGCTCCAGTAGGTTCATCACCAAACAAAATTTTAGGTTCTTTAATTAAAGCCCGAGCAATAGCAACACGTTGTTGTTGTCCACCAGAGAGTTTATGAACTTTCTTATGTCTATGTTCTTTTAATTCCAAGCGTTCTAATAATTCTTCAAGATTATTATTAAACCTTTTTTTTAATGGTAGAGCAAGCTTAATATTATCATCAACGTCAAGATCACGCAATAAACCATATTGTTGAAAAATATAACCAACGTTTTTATTTCTTAACGCGGTTAGTTTTGCATCACTACAACATATGGTGTTAGTTCCACAAACAAAACTATCGCCGCTAGTTGGTCGATCTAATGCGGAAATTAAAGAAAGTAAGCTGGTTTTACCACTACCAGATTTACCAAGAATAACAACAAATTCTCCTGGAAGAATTTTTAAATTAATGTGGTTACAAATAAGTTGGTTGTTAATGCCATTTGTAACAGCTTTACAAACTTCTTTGAAGTAAATATCATACTTTTTAGCTTCAAATGAATTAAGTGATTTTCTTTTGGGGTGCTTTTTATTTAACTTTTTAATTAATTTAGATGCTTTTTTACTAACAGCAAAATCTGATGATTTTAAGTAAATATCAAACTTTTTGTTCTCTTTTGTTTTTAAAACCATAAACAATCATTAAGTCATCTAAAAAAATACCCTGAGAACACTATTAGAAAATTTTAATTTTGCTAAATTACTAATGATGTAATTACATTCCGTAATGAACTGTCATTTTCAATCATTTTAGCAACTCTTTTAACTGCCATTAATACACTTGAATGACTTCTCTTAAATATTTTGCCAATTTGTTGAAATTGCATGTTGTACTTTTGACGCAAAAGGTAATTACACACATCACGAACACGGACAAGTTCTGCTTTACGATTTTCTGAAAGTACACTGTCCATAGGAACATTAAATCTACGGCAAACGTTCTCTATTAATAAATAAGGATCAAAGCTCTTTTTATGAAACTTCTCAAATTCTTCAAAAAGAATTTCTTTTAAATTTTCAGTATTTATTAAATTTTGTTTTGAAGTTTTAGCAAAAAATAATAACTTTGTTGCAATTCCATTTAAAGCACGCACATCGTTACCTGAAATTTGTGCTGCATCATGTCTTGCCTCATTAGTTATTTGGATGTTAGGATCTTTTTCTTTTAATTTAACAGTAAGTATTTCACAAAGTGAAGACAAATTATGCTTTTCTATCTTTAGTAATAATCCTGATTTAAAGCGAGAAATCATTCTTGCATCAATATCAATTAGTTCATCAGGAGCCTTATCAGAAACTAAAACAATTTGTTTTTTATTTAGAACTAGGTTATTAAAAATATTGAAAAGAATTTCTAAGGTTTTTTCTTTTCTGCCAAATATTTGAGTGTCATCTATTAAAACTAAATCTAAATTTTCATAATTTTTTTTTAGTTTTTCTATACCTTTATCCCTTTGATAAAAAGCATCAACAACTTCTTGGGCAAAATCACTTGAAACAACATACTTTACTCTGGCATTTGGAAAATTACGAAATTTTTCATTTCCTATTGCTTGTAGTAGGTGAGTTTTACCAAGACCGGTTTCTCCGTAAATAAAAAGCGGTGAAAATTCGTTATCTTGAGTTTCAGCTAATCTAACGCCTGCTTCATAAGCTCTTTTATTTCCTTCACTAATTACAAAGTTTTGAAAAGTATAGTTTTTACTCAATCCAGAATTTTGATAAAGAGTATCACGACTATTTTCTTCTAATTTTGCTAAGTTAAAAAAGAAATCTTGCTCATTAACAAAATTAACACTTTTAATTCCTTCATACAAACTTTCAGCTAAATGAATAATTTCAGAATTATCATTTAAGGAATTACGAGCAAATTCATTTTCTAAAAGAATTAAAAGAACATTATTTTTAAATACGAAACGATTAATGTCTTTAATGTATTTATCATGAAAACCTATTGTTTTTTCATAATGCTTTTTTAATAGAGATTTAAAGGCATTAAATTGTTCCATTATTCTTCTATAACATTGTCAAGAATGATAGTTAAAATTCTCGAAATTGGGATATTAACTGCTTTGGAGTAATTTCTAACTTTTTGTCATACTCTTTGACTTGTATAGAAGTGTACACCTGTATCTAGTTTTTCTTGGCGTTCAACAGGAACTATTCCTGGTATTTTTGTTTTAGGTTGGGGAGGAATAGGCTGTGGTTGTGTGAATTGTTGTTGAAAATTTTGATTTTTTTGCTGTAAGAAACCATTATTATGATATTGAAAATTTTGTTCCTCTTTAAAATATCTCTCTTTTTTTGGTTTTCCAGAAAAATTTGATGAAAAAGATTTTTCTTCATTTCAATTTTCAAGATTATTTTCATTTTGTTGATTTATTTGCTCAGGCTGTTGAAATGAATTATTTTTTGATCAAAAAGATTTTGGAAAGGTTTTTTCAAAAGCAGATAAAGGTCCAAAATCAAATGAAGATGAATCTTTGTCAAAAGATGTTTCTTCTCTTTTTGACAAATTTTGTTTTTGATTAAACTTATTTTTATTTTGGGGTGTTACTTTTTCTTTTATGGAAAACAAATCTTCTTCTAAAAGACTTTGTTCTGGGTCATCATCTTGTGCTAAATCAAAGAAAAAACGTTTCTTTTTGTTATTAATGGACATTGTAATTTGCTAAATTTAGGATTTCTTTTGTTATTTCTAAATACTCATTTAGATATTTTTTACTTGGTGATGATACTAATGATATTGGCAATTTTTCATAACCTACAGCTGCTGATGATTTTGATGTCAGAGAAACAAAATTTTTAGAAAAAGCTACATTATTTTTTTTAGCTTTTGTTTTAGCTAAATCTATTACTTCATTATGAAGACGAGTACGAACGTTAACTTTTGTAGGAACTAAAATAGTTTTAAGATTTGTATTTTTTTCCTTAAATGTATCTATTGTTTCAACTATTCTCATCAAACCTAGCATCGAATATTGATCTGGTTCAAAGGGAATAACTATGACATCTGATAAACTCATTGCAGTAGAAACTAAAGTTGCCATATTTGGTGGTGTATCTAATAAAACAAATTCATATCTTTTTGCTAGTTGCTTAACTATTTCTGCTATATCTGAGGCCTTATATTTTTTACGTGATATGTCTATATCAGCAAAATTAAGTTCAAAATTACAAGGAAGAATATCAAGTCCCTCATATACAGATAGCAAGCAATCATCTATTTCAATGAAATTATTTGAACCACTGAATTTTGGAACCTTCAACAAAATGTCAATTAACGTGTTATTCAATCTTTCAGGGTTTTGTCCAAATGATGCAGAAACATTCCCCTGCCCGTCAAGATCAAGAATGACTTTTCGCCTTTCTGGACAAAGTTTAACCAATGATCCTGCAACATTAGTTGCCATTGTAGTTTTTAATACGCCGCCTTTATTATTTACAAAAGAAATGATCATATATTTAAATGATTATAATATTTCTTTAATACTAAAAAAATAC", "length": 579558, "complete": true, "type": "contig", "topology": "circular", "simple_id": "contig_1", "orig_id": "GCA_000292505.1|CP003773.1", "orig_description": "HMT-616 Mycoplasmoides genitalium M2288" } ], "run": { "start": "2026-01-29 10:57:50", "end": "2026-01-29 11:06:11", "duration": "8.34 min" }, "version": { "bakta": "1.11.4", "db": { "version": "6.0", "type": "full" } } }